LOCUS       DAJTMC010000001        89996 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.001, whole genome shotgun sequence.
ACCESSION   DAJTMC010000001 DAJTMC010000000
VERSION     DAJTMC010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 89996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 89996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..89996
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.001"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            <1..791
                     /locus_tag="OO130_000001"
     CDS             <1..791
                     /locus_tag="OO130_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5363563.1"
                     /translation="DSLDIKTTLQANDIPGLTTDQVVSVNTRFTQIKSSTVYSAATQT
                     GVCQGDTSRYGPVNIGANTTFTLYVTKPFLGSMTIPKTDIAVIKGVWVDGMGSPSTGD
                     FHDLVKLSIQGNLTAPQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITY
                     DCGDTSKIKNSLQMRIDGTTGVVDQYNLVARRRSSDNVPDVGIRIENLGGGVANIPFQ
                     NGILPVDPSGHGTVNMRAWPVNLVGGELETGKFQGTATITVMVR"
     gene            complement(834..1694)
                     /gene="rsmI"
                     /locus_tag="OO130_000002"
     CDS             complement(834..1694)
                     /gene="rsmI"
                     /locus_tag="OO130_000002"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HCR5363564.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALVAEIHGVKKNA
                     LYKYALEQQG"
     gene            1758..3785
                     /gene="lpoA"
                     /locus_tag="OO130_000003"
     CDS             1758..3785
                     /gene="lpoA"
                     /locus_tag="OO130_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HCR5363565.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAVEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKKQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAADVAEQPQPQTADGVASPAQASVSDLTGDQPAAQPVP
                     VSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNVEEL
                     LKSNTPLNVLVLNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIPRSS
                     LGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGTPITPRATTDSG
                     MTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGAMLYAS
                     SRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMGVDA
                     WSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            3743..4138
                     /locus_tag="OO130_000004"
     CDS             3743..4138
                     /locus_tag="OO130_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HCR5363566.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEVQARRWLEGKGLRFVAANVN
                     ERGGEIDLIMREGWTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            4158..4748
                     /gene="diaA"
                     /locus_tag="OO130_000005"
     CDS             4158..4748
                     /gene="diaA"
                     /locus_tag="OO130_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HCR5363567.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            4758..5333
                     /gene="yraP"
                     /locus_tag="OO130_000006"
     CDS             4758..5333
                     /gene="yraP"
                     /locus_tag="OO130_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HCR5363568.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(5453..6004)
                     /locus_tag="OO130_000007"
                     /pseudo
     CDS             complement(5453..6004)
                     /locus_tag="OO130_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312059.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
     gene            complement(6077..6712)
                     /locus_tag="OO130_000008"
     CDS             complement(6077..6712)
                     /locus_tag="OO130_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HCR5363569.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            6840..7358
                     /gene="yhbO"
                     /locus_tag="OO130_000009"
     CDS             6840..7358
                     /gene="yhbO"
                     /locus_tag="OO130_000009"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HCR5363570.1"
                     /translation="MSKKIAVLISDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(7338..7781)
                     /locus_tag="OO130_000010"
     CDS             complement(7338..7781)
                     /locus_tag="OO130_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HCR5363571.1"
                     /translation="METLITISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     HRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            7832..8134
                     /gene="yhbQ"
                     /locus_tag="OO130_000011"
     CDS             7832..8134
                     /gene="yhbQ"
                     /locus_tag="OO130_000011"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HCR5363572.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(8121..8624)
                     /locus_tag="OO130_000012"
     CDS             complement(8121..8624)
                     /locus_tag="OO130_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR5363573.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(8618..9142)
                     /locus_tag="OO130_000013"
     CDS             complement(8618..9142)
                     /locus_tag="OO130_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HCR5363574.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADVSDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLHVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            9351..10331
                     /locus_tag="OO130_000014"
     CDS             9351..10331
                     /locus_tag="OO130_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCR5363575.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPNIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            10340..11218
                     /locus_tag="OO130_000015"
     CDS             10340..11218
                     /locus_tag="OO130_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCR5363576.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAATSSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELTSMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLLLTANS
                     DCNGYWRRLAGLELQA"
     gene            11424..12431
                     /locus_tag="OO130_000016"
     CDS             11424..12431
                     /locus_tag="OO130_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase-like monooxygenase"
                     /protein_id="HCR5363577.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(12549..13793)
                     /gene="mtr"
                     /locus_tag="OO130_000017"
     CDS             complement(12549..13793)
                     /gene="mtr"
                     /locus_tag="OO130_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HCR5363578.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAMGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMITLIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(13947..15836)
                     /gene="deaD"
                     /locus_tag="OO130_000018"
     CDS             complement(13947..15836)
                     /gene="deaD"
                     /locus_tag="OO130_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HCR5363579.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(15829..15909)
                     /gene="yrbN"
                     /locus_tag="OO130_000019"
     CDS             complement(15829..15909)
                     /gene="yrbN"
                     /locus_tag="OO130_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HCR5363580.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(16016..16900)
                     /gene="nlpI"
                     /locus_tag="OO130_000020"
     CDS             complement(16016..16900)
                     /gene="nlpI"
                     /locus_tag="OO130_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HCR5363581.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDSDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(17009..19144)
                     /gene="pnp"
                     /locus_tag="OO130_000021"
     CDS             complement(17009..19144)
                     /gene="pnp"
                     /locus_tag="OO130_000021"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HCR5363582.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRSVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSREGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(19392..19661)
                     /gene="rpsO"
                     /locus_tag="OO130_000022"
     CDS             complement(19392..19661)
                     /gene="rpsO"
                     /locus_tag="OO130_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HCR5363583.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(19810..20754)
                     /gene="truB"
                     /locus_tag="OO130_000023"
     CDS             complement(19810..20754)
                     /gene="truB"
                     /locus_tag="OO130_000023"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HCR5363584.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICFGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITIYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(20754..21155)
                     /gene="rbfA"
                     /locus_tag="OO130_000024"
     CDS             complement(20754..21155)
                     /gene="rbfA"
                     /locus_tag="OO130_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HCR5363585.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(21319..24015)
                     /gene="infB"
                     /locus_tag="OO130_000025"
     CDS             complement(21319..24015)
                     /gene="infB"
                     /locus_tag="OO130_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HCR5363586.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREA
                     AEKDKVSNQQDDMTKNAQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMA
                     EENKWTDNAEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKH
                     AESKADREEARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMA
                     VKGSQVIKAMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTG
                     AAAEPRAPVVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITF
                     LDTPGHAAFTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKI
                     DKPEADPDRVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELK
                     AVRKGMASGAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQE
                     VLEAGPSIPVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKL
                     ENMFANMTEGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETD
                     ATLAAASNAILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPEL
                     KQQIIGLAEVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRF
                     KDDVNEVRNGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(24040..25527)
                     /gene="nusA"
                     /locus_tag="OO130_000026"
     CDS             complement(24040..25527)
                     /gene="nusA"
                     /locus_tag="OO130_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HCR5363587.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLELGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGIDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGVTDEKAGALIMAARNICWFGDEA"
     gene            complement(25555..26007)
                     /gene="rimP"
                     /locus_tag="OO130_000027"
     CDS             complement(25555..26007)
                     /gene="rimP"
                     /locus_tag="OO130_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HCR5363588.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGHTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(26214..26290)
                     /locus_tag="OO130_000028"
     tRNA            complement(26214..26290)
                     /locus_tag="OO130_000028"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(26254..26256),aa:Met,seq:cat)
     gene            26638..27981
                     /gene="argG"
                     /locus_tag="OO130_000029"
     CDS             26638..27981
                     /gene="argG"
                     /locus_tag="OO130_000029"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HCR5363589.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSNDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGVPQVENMENKGQ"
     gene            complement(27989..29614)
                     /locus_tag="OO130_000030"
     CDS             complement(27989..29614)
                     /locus_tag="OO130_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HCR5363590.1"
                     /translation="MTVFNKFARSFKSHWLLYLCVIVFGITNLVASSGAHMVQRLLFF
                     VLTILVVKRISSLPLRLLVAAPFVLLTAADMSISLYSWCTFGTTFNDGFAISVLQSDP
                     DEVVKMLGMYIPYLCAFAFLSLLFWAVIIKYDVSLPTKKVTGILLLIVISGSLFSACQ
                     FAYKDAKNKKTFSPYILASRFATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVR
                     DTGIDTYVLIVGESVRVDNMSLYGYTRSTTPQVEAQRKQIKLFNQAISGAPYTALSVP
                     LSLTADSVLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETV
                     YVRGFDELLLPHLSQALQQNTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDAC
                     YDNSIHYTDSLLGQVFELLKDRRASVMYFADHGLERDPTKKNVYFHGGREASQQAYHV
                     PMFIWYSPVLGDGVDRTTENNIFSTAYNNYLINAWMGVTKPEQPQTLEEVIAHYKGDS
                     RVVDANHDVFDYVMFRKEFTEDKQGNPPPEGQG"
     gene            complement(30073..30159)
                     /locus_tag="OO130_000031"
     tRNA            complement(30073..30159)
                     /locus_tag="OO130_000031"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(30123..30125),aa:Leu,seq:gag)
     gene            complement(30174..30506)
                     /gene="secG"
                     /locus_tag="OO130_000032"
     CDS             complement(30174..30506)
                     /gene="secG"
                     /locus_tag="OO130_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HCR5363591.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(30734..32071)
                     /gene="glmM"
                     /locus_tag="OO130_000033"
     CDS             complement(30734..32071)
                     /gene="glmM"
                     /locus_tag="OO130_000033"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HCR5363592.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(32064..32912)
                     /gene="folP"
                     /locus_tag="OO130_000034"
     CDS             complement(32064..32912)
                     /gene="folP"
                     /locus_tag="OO130_000034"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HCR5363593.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWLSVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(33002..34936)
                     /gene="ftsH"
                     /locus_tag="OO130_000035"
     CDS             complement(33002..34936)
                     /gene="ftsH"
                     /locus_tag="OO130_000035"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HCR5363594.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMIEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(35036..35665)
                     /gene="rlmE"
                     /locus_tag="OO130_000036"
     CDS             complement(35036..35665)
                     /gene="rlmE"
                     /locus_tag="OO130_000036"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HCR5363595.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            35791..36084
                     /gene="yhbY"
                     /locus_tag="OO130_000037"
     CDS             35791..36084
                     /gene="yhbY"
                     /locus_tag="OO130_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HCR5363596.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(36241..36717)
                     /gene="greA"
                     /locus_tag="OO130_000038"
     CDS             complement(36241..36717)
                     /gene="greA"
                     /locus_tag="OO130_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HCR5363597.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            36965..38398
                     /gene="dacB"
                     /locus_tag="OO130_000039"
     CDS             36965..38398
                     /gene="dacB"
                     /locus_tag="OO130_000039"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HCR5363598.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMIATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVIPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(38438..39610)
                     /gene="cgtA"
                     /locus_tag="OO130_000040"
     CDS             complement(38438..39610)
                     /gene="cgtA"
                     /locus_tag="OO130_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HCR5363599.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(39626..40591)
                     /locus_tag="OO130_000041"
     CDS             complement(39626..40591)
                     /locus_tag="OO130_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR5363600.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(40718..40975)
                     /gene="rpmA"
                     /locus_tag="OO130_000042"
     CDS             complement(40718..40975)
                     /gene="rpmA"
                     /locus_tag="OO130_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HCR5363601.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(40996..41307)
                     /gene="rplU"
                     /locus_tag="OO130_000043"
     CDS             complement(40996..41307)
                     /gene="rplU"
                     /locus_tag="OO130_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HCR5363602.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            41566..42537
                     /gene="ispB"
                     /locus_tag="OO130_000044"
     CDS             41566..42537
                     /gene="ispB"
                     /locus_tag="OO130_000044"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HCR5363603.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            42765..43043
                     /gene="sfsB"
                     /locus_tag="OO130_000045"
     CDS             42765..43043
                     /gene="sfsB"
                     /locus_tag="OO130_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HCR5363604.1"
                     /translation="MESNFIDWHPPDIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(43091..44350)
                     /gene="murA"
                     /locus_tag="OO130_000046"
     CDS             complement(43091..44350)
                     /gene="murA"
                     /locus_tag="OO130_000046"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCR5363605.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIKRVKGE"
     gene            complement(44405..44659)
                     /gene="ibaG"
                     /locus_tag="OO130_000047"
     CDS             complement(44405..44659)
                     /gene="ibaG"
                     /locus_tag="OO130_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HCR5363606.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(44819..45112)
                     /gene="mlaB"
                     /locus_tag="OO130_000048"
     CDS             complement(44819..45112)
                     /gene="mlaB"
                     /locus_tag="OO130_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HCR5363607.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(45112..45747)
                     /gene="mlaC"
                     /locus_tag="OO130_000049"
     CDS             complement(45112..45747)
                     /gene="mlaC"
                     /locus_tag="OO130_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HCR5363608.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(45766..46317)
                     /gene="mlaD"
                     /locus_tag="OO130_000050"
     CDS             complement(45766..46317)
                     /gene="mlaD"
                     /locus_tag="OO130_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HCR5363609.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(46322..47104)
                     /gene="mlaE"
                     /locus_tag="OO130_000051"
     CDS             complement(46322..47104)
                     /gene="mlaE"
                     /locus_tag="OO130_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HCR5363610.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(47112..47921)
                     /gene="mlaF"
                     /locus_tag="OO130_000052"
     CDS             complement(47112..47921)
                     /gene="mlaF"
                     /locus_tag="OO130_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HCR5363611.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGCAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            48131..49108
                     /locus_tag="OO130_000053"
     CDS             48131..49108
                     /locus_tag="OO130_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HCR5363612.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            49122..50108
                     /gene="kdsD"
                     /locus_tag="OO130_000054"
     CDS             49122..50108
                     /gene="kdsD"
                     /locus_tag="OO130_000054"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HCR5363613.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            50129..50695
                     /gene="kdsC"
                     /locus_tag="OO130_000055"
     CDS             50129..50695
                     /gene="kdsC"
                     /locus_tag="OO130_000055"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HCR5363614.1"
                     /translation="MSKAGASLATCYGPVSADVIAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSELLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            50692..51267
                     /gene="lptC"
                     /locus_tag="OO130_000056"
     CDS             50692..51267
                     /gene="lptC"
                     /locus_tag="OO130_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HCR5363615.1"
                     /translation="MSKARRWVTIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            51236..51793
                     /gene="lptA"
                     /locus_tag="OO130_000057"
     CDS             51236..51793
                     /gene="lptA"
                     /locus_tag="OO130_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HCR5363616.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            51800..52525
                     /gene="lptB"
                     /locus_tag="OO130_000058"
     CDS             51800..52525
                     /gene="lptB"
                     /locus_tag="OO130_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HCR5363617.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            52573..54006
                     /gene="rpoN"
                     /locus_tag="OO130_000059"
     CDS             52573..54006
                     /gene="rpoN"
                     /locus_tag="OO130_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HCR5363618.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTSETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            54029..54316
                     /gene="hpf"
                     /locus_tag="OO130_000060"
     CDS             54029..54316
                     /gene="hpf"
                     /locus_tag="OO130_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HCR5363619.1"
                     /translation="MQLNITGNNVEITEALREFVRAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            54434..54925
                     /gene="ptsN"
                     /locus_tag="OO130_000061"
     CDS             54434..54925
                     /gene="ptsN"
                     /locus_tag="OO130_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HCR5363620.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            54971..55825
                     /gene="rapZ"
                     /locus_tag="OO130_000062"
     CDS             54971..55825
                     /gene="rapZ"
                     /locus_tag="OO130_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HCR5363621.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            55822..56094
                     /gene="npr"
                     /locus_tag="OO130_000063"
     CDS             55822..56094
                     /gene="npr"
                     /locus_tag="OO130_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HCR5363622.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            56308..56940
                     /gene="yrbL"
                     /locus_tag="OO130_000064"
     CDS             56308..56940
                     /gene="yrbL"
                     /locus_tag="OO130_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HCR5363623.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAYLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHHISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(56937..57665)
                     /gene="mtgA"
                     /locus_tag="OO130_000065"
     CDS             complement(56937..57665)
                     /gene="mtgA"
                     /locus_tag="OO130_000065"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HCR5363624.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPEHRGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSSPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(57662..58315)
                     /gene="elbB"
                     /locus_tag="OO130_000066"
     CDS             complement(57662..58315)
                     /gene="elbB"
                     /locus_tag="OO130_000066"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HCR5363625.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFATLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(58545..60881)
                     /gene="arcB"
                     /locus_tag="OO130_000067"
     CDS             complement(58545..60881)
                     /gene="arcB"
                     /locus_tag="OO130_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HCR5363626.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(60977..61906)
                     /locus_tag="OO130_000068"
     CDS             complement(60977..61906)
                     /locus_tag="OO130_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HCR5363627.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            62581..67041
                     /gene="gltB"
                     /locus_tag="OO130_000069"
     CDS             62581..67041
                     /gene="gltB"
                     /locus_tag="OO130_000069"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HCR5363628.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKALYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLNVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            67054..68472
                     /gene="gltD"
                     /locus_tag="OO130_000070"
     CDS             67054..68472
                     /gene="gltD"
                     /locus_tag="OO130_000070"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HCR5363629.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEQFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENKDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            68656..69135
                     /locus_tag="OO130_000071"
     CDS             68656..69135
                     /locus_tag="OO130_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005085993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HCR5363630.1"
                     /translation="MESLSERTSTGYQQIHDGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIKRLSKDLSLRYKRGFSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIPQTPSAEFPSAKIWQTLSAKLRGCLIFPQLLYCISYFRFVYSCK"
     gene            69152..69457
                     /locus_tag="OO130_000072"
     CDS             69152..69457
                     /locus_tag="OO130_000072"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5363631.1"
                     /translation="MIYIEQLELIHKSGDVLYPVKITRKSSGKTAFHLVPFGLNKTHD
                     LLEVEDASEAIRLVIDERHSIRCSTLTATITNKKGKRIKRTGIYSIKGVNIKEYNVR"
     gene            complement(69533..69997)
                     /locus_tag="OO130_000073"
     CDS             complement(69533..69997)
                     /locus_tag="OO130_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HCR5363632.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSTIDNEQAIILKPGMFAVFMPSEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(69994..70869)
                     /gene="nanK"
                     /locus_tag="OO130_000074"
     CDS             complement(69994..70869)
                     /gene="nanK"
                     /locus_tag="OO130_000074"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HCR5363633.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPQALRDA
                     LAALVAPLQAHAQQVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLSNLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELMGADARTIFTRAGQGDEQAQQLIHRSA
                     HVLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(70866..71555)
                     /locus_tag="OO130_000075"
     CDS             complement(70866..71555)
                     /locus_tag="OO130_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HCR5363634.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSDAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(71603..73093)
                     /gene="nanT"
                     /locus_tag="OO130_000076"
     CDS             complement(71603..73093)
                     /gene="nanT"
                     /locus_tag="OO130_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HCR5363635.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVSGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(73202..74095)
                     /gene="nanA"
                     /locus_tag="OO130_000077"
     CDS             complement(73202..74095)
                     /gene="nanA"
                     /locus_tag="OO130_000077"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HCR5363636.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(74217..75008)
                     /gene="nanR"
                     /locus_tag="OO130_000078"
     CDS             complement(74217..75008)
                     /gene="nanR"
                     /locus_tag="OO130_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HCR5363637.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
     gene            75388..76754
                     /gene="dcuC"
                     /locus_tag="OO130_000079"
                     /pseudo
     CDS             75388..76754
                     /gene="dcuC"
                     /locus_tag="OO130_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417694.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
     gene            complement(76797..77294)
                     /gene="sspB"
                     /locus_tag="OO130_000080"
     CDS             complement(76797..77294)
                     /gene="sspB"
                     /locus_tag="OO130_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HCR5363638.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(77300..77938)
                     /gene="sspA"
                     /locus_tag="OO130_000081"
     CDS             complement(77300..77938)
                     /gene="sspA"
                     /locus_tag="OO130_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HCR5363639.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(78333..78725)
                     /gene="rpsI"
                     /locus_tag="OO130_000082"
     CDS             complement(78333..78725)
                     /gene="rpsI"
                     /locus_tag="OO130_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HCR5363640.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(78741..79169)
                     /gene="rplM"
                     /locus_tag="OO130_000083"
     CDS             complement(78741..79169)
                     /gene="rplM"
                     /locus_tag="OO130_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HCR5363641.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKCTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(79387..80514)
                     /gene="zapE"
                     /locus_tag="OO130_000084"
     CDS             complement(79387..80514)
                     /gene="zapE"
                     /locus_tag="OO130_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HCR5363642.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLHDETRAQMDKLWLALAGAKRENSPTLEINHRPLATMGAENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGDRLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            80708..81106
                     /locus_tag="OO130_000085"
     CDS             80708..81106
                     /locus_tag="OO130_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HCR5363643.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            81260..82627
                     /gene="degQ"
                     /locus_tag="OO130_000086"
     CDS             81260..82627
                     /gene="degQ"
                     /locus_tag="OO130_000086"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HCR5363644.1"
                     /translation="MKKQTQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDCGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIVRGNESI
                     YLLMR"
     gene            82717..83784
                     /gene="degS"
                     /locus_tag="OO130_000087"
     CDS             82717..83784
                     /gene="degS"
                     /locus_tag="OO130_000087"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HCR5363645.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGVNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(83847..84785)
                     /gene="mdh"
                     /locus_tag="OO130_000088"
     CDS             complement(83847..84785)
                     /gene="mdh"
                     /locus_tag="OO130_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCR5363646.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPSEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
     gene            85220..85690
                     /gene="argR"
                     /locus_tag="OO130_000089"
     CDS             85220..85690
                     /gene="argR"
                     /locus_tag="OO130_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HCR5363647.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            86055..86318
                     /gene="yhcN"
                     /locus_tag="OO130_000090"
     CDS             86055..86318
                     /gene="yhcN"
                     /locus_tag="OO130_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HCR5363648.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(86373..86645)
                     /locus_tag="OO130_000091"
     CDS             complement(86373..86645)
                     /locus_tag="OO130_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HCR5363649.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(86737..88704)
                     /gene="aaeB"
                     /locus_tag="OO130_000092"
     CDS             complement(86737..88704)
                     /gene="aaeB"
                     /locus_tag="OO130_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HCR5363650.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IIADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQQRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTERVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            complement(88710..89642)
                     /gene="aaeA"
                     /locus_tag="OO130_000093"
                     /pseudo
     CDS             complement(88710..89642)
                     /gene="aaeA"
                     /locus_tag="OO130_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417708.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
     gene            complement(89650..89853)
                     /locus_tag="OO130_000094"
     CDS             complement(89650..89853)
                     /locus_tag="OO130_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AaeX family protein"
                     /protein_id="HCR5363651.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
BASE COUNT        22132 a        24162 c        22362 g        21340 t
ORIGIN      
        1 atgacagcct ggatattaaa accacattac aggcaaacga cattccagga ctcacaaccg
       61 accaggttgt ctctgttaac acccgattca cacagataaa aagcagcacc gtatattctg
      121 ctgcaaccca aacgggtgtt tgccagggtg acacgtctcg ttatggaccc gttaatattg
      181 gtgcaaatac cacctttacc ctgtatgtca ccaagccatt tctcggctcg atgaccattc
      241 cgaaaacgga tattgccgtc attaaaggcg tgtgggtcga tggaatggga agcccgtcta
      301 caggtgactt ccatgattta gtcaagttat cgattcaggg aaatctcacc gccccacagt
      361 cgtgcaaaat taatcagggc gatgttatta aggttaattt tggattcatc aatggtcaga
      421 agtttaccac ccgcaatgcc atgccagacg gttttactcc agtagacttt gatatcactt
      481 atgactgtgg tgatacttca aagattaaaa actcgttgca aatgcgcatc gacggtacaa
      541 ctggggtagt agaccagtac aacctggtcg ccaggcgaag aagttcagac aatgtgcccg
      601 atgtcggtat tcgtattgaa aatctcggcg gcggagttgc aaatattcct tttcagaacg
      661 gtatccttcc cgttgatcct tccgggcatg gcaccgtcaa tatgcgcgcc tggccagtta
      721 atctggtcgg tggtgagctg gaaacaggaa aatttcaggg cacagccacc attaccgtca
      781 tggtgcggta aaagaagacg ttgcgttttg attaacgcaa cgcttcggca ctgttacccc
      841 tgctgctcca gcgcatactt atacagcgca tttttcttca cgccatgaat ttctgcgacc
      901 agcgccgccg cttttttcag cggcagttct gcctgtagca gcgccagcgt gcgcagggca
      961 tcggcgggta agtcttcttc ctgtgcttta tgaccttcga caatcagcac catttcgcct
     1021 ttgcgacggt tttcatcttc ctttacccac gccagcagtt cgccaacggg cgcgccgtga
     1081 atggtttccc aggttttggt cagctcacgc gccagaacca cgtagcggga ttcgcctaat
     1141 accgcaacga tatcttccag gctatctaac agacggtggg tggattcata aaaaatcagc
     1201 gtgcgcggct ccgcttcaat ggcttttagc gcatcacggc ggccttttga tttggcaggt
     1261 aaaaagcctt cgtaacagaa acggtcagag ggtaaacccg cggcgcttaa cgcagtgata
     1321 gcagcacacg gcccgggtag cggcaccacg cgaatccccg cttcacggca ggtacgcacc
     1381 agatggtagc caggatcgtt aattagcggc gttccggcat cggaaaccag cgcaatgttt
     1441 tgcccctctt gcagcttcgc cagcagcgtt tcggcttttt gttgttcgtt atggtcgtgc
     1501 agcgcaaaca accgggcatt aatcccaaaa tgttgcagca ataaaccggt gtgacgagta
     1561 tcctcggcgg caatcagatc aacggcctgt aatacctcta acgcacgctg ggtgatatcc
     1621 gccagattgc cgattggcgt cggtacaatg taaagctggc cctgagaatt atccgccgat
     1681 tggtgttgtt tcattgtgtc gtccgtattg ccgatttaat attgagcatt gcgtaaaaaa
     1741 atatcactgg atacattatg gtaccctcaa cattttctcg tttgaaagcc gcgcgttgtc
     1801 tgcctgttgt tctggcagcc ctgattttcg ccggttgtgg cacccatact cccgatcagt
     1861 ccactgctta tatgcagggc acagcgcagg ctgattctgc cttttatctt cagcagatgc
     1921 agcaaagctc tgatgatacc aggatcaact ggcaattact cgccattcgt gcactggtga
     1981 aagaaggtaa aaccgggcag gcggttgagt tgtttaacca actaccgcaa gaactgaacg
     2041 attctcagcg tcgcgagaaa acactgctgg cggtagagat taaactggcg cagaaagatt
     2101 ttgctggcgc gcaaaacttg ctggcgaaaa tcacacctgc cgatttagaa caaaaccagc
     2161 aagcgcgtta ctggcaggca aaaatcgatg ccagccaggg gcgtccttcc attgatttac
     2221 tgcgcgcgtt aattgctcag gaaccgctgc tcggcgcgaa agaaaaaaag cagaatattg
     2281 atgccacctg gcaggcgctc tcctccatga ctcaggaaca ggcgaatacg ctggtgatca
     2341 acgccgacga aaatattctg caaggctggc tggatctgca gcgcgtctgg tttgataacc
     2401 gtaacgatcc cgatatgatg aaagccggga tcgccgactg gcagaaacgt tatccgaaca
     2461 atccgggcgc gaaaatgctg ccaacgcagt tggttaacgt aaaagcgttt aaaccagcct
     2521 cgaccaacaa aatcgccctg ctgttgccgc tgaatggcca ggcagcggta tttggtcgca
     2581 ctattcagca aggctttgaa gcggcgaaaa atatcggcac tcagccagtg gcggctcagg
     2641 tagctgccgc agacgtagca gaacaacctc agccgcaaac tgcggatggc gttgccagcc
     2701 cggcacaagc ctcggttagc gatctgaccg gtgatcagcc tgcggcccag ccggtgcctg
     2761 taagcgcccc ggcgacaagc accgcagcgg taagcgcacc cgcaaatcca tccgcagagc
     2821 tgaaaatcta cgatacctca tcacaaccac ttagccagat cttaagccag gttcagcagg
     2881 atggcgcgag tattgtggtt ggtccgttgc tgaaaaataa cgttgaagag ttgcttaaga
     2941 gcaacacccc gctgaacgtg ctggtactga accagccgga gaatatcgaa aaccgcgtca
     3001 atatttgtta cttcgcgctt tcaccggaag acgaagcgcg cgatgcagcg cgtcatattc
     3061 gtgaccaggg taaacaagcg ccgctggtgc tgatcccacg cagttcattg ggcgatcgcg
     3121 tagccaatgc gtttgcgcag gagtggcaga aactgggcgg cggcaccgtt ctgcaacaaa
     3181 aatttggttc caccagcgaa ttacgcgcgg gtgttaacgg cggttctggt atcgctttaa
     3241 cgggtacccc gattactccc agagcgacaa ccgactccgg catgacgacc aacaatccaa
     3301 cgctgcaaac cacgccaacc gatgaccagt tcaccaataa tggcggtcgt gtcgatgcgg
     3361 tgtacattgt ggcaacgccg ggtgaaatcg cttttatcaa accgatgatc gccatgcgta
     3421 acggtagcca gagcggtgca atgctgtacg ccagctcccg cagcgcacaa ggcaccgcag
     3481 gcccggattt ccgtctggag atggaaggtt tgcagtacag cgaaatcccg atgctggcgg
     3541 gcggtaatct gccgttaatg cagcaggcac tcagcgcggt gaataacgat tattcactgg
     3601 ctcgcatgta tgcgatgggc gtcgatgcct ggtcgctggc aaatcatttc tcacaaatgc
     3661 gccaggttca gggttttgaa atcaacggta ataccggaag cctgacggca aacccggatt
     3721 gcgtgattaa caggaagtta tcatggctac agtaccaaca aggtcaggta gtccccgcca
     3781 gttaaccacc aaacagaccg gcgatgcgtg ggaagtacaa gcgcgtcgct ggctggaagg
     3841 caaaggactg cgatttgtcg ccgctaacgt gaacgagcgt ggcggtgaga tcgatctgat
     3901 aatgcgtgaa ggctggacca ccgtttttgt cgaggtgcgt taccgccgct ctgcgcttta
     3961 tggcggcgcg gcagccagtg tgacccgcag caaacaacac aaattattac agactgcccg
     4021 cttgtggctc gcgcgtcata atgggagttt tgatactgtg gattgccggt tcgatgtggt
     4081 agccttcacc gggaatgagg ttgagtggat taaggatgcc tttaatgacc actcataatt
     4141 aaggtttaag gattagcgtg caagaaagaa ttaaagcttg cttcactgaa agcattcaaa
     4201 ctcaaattgc ggcggcagag gcgcttccgg atgccatctc ccgtgcagcc atgacgctgg
     4261 ttcagtctct gctcaatggc aacaaaatcc tctgttgtgg taatggaact tccgctgcca
     4321 atgcacagca ttttgctgcc agcatgatca accgtttcga aacggagcgg cccagcttac
     4381 ctgccattgc actaaatact gataatgttg tcttaacggc gattgccaac gatcgcttac
     4441 atgatgaagt gtatgcaaaa caggtgcggg cgctgggtca tgcgggagat gtattgttag
     4501 ccatttccac ccgtggcaac agccgcgata ttgttaaagc agttgaagcc gccgttacgc
     4561 gtgatatgac cattgtggca ttgaccggct atgacggcgg cgaacttgca ggtttgttag
     4621 ggccacagga tgtggagatc cgcattcctt cgcatcgtag tgctcgcatt caggaaatgc
     4681 atatgctgac ggtaaattgc ctgtgcgatc tgatcgataa cacgcttttc cctcaccagg
     4741 atgattaagg agaatacatg aaggcattat cgccaatcgc agtccttatt tccgcgctgc
     4801 tgttgcaagg ttgtgttgcc gctgccgtag tgggtaccgc tgctgtgggt accaaagccg
     4861 caactgaccc acgcagtgtc ggtacccagg tggacgatgg taccctggaa gtgcgcgtga
     4921 acagcgcatt gtcgaaagac gaacagatta agaaagaaac ccgtattaat gtcacggcgt
     4981 atcagggcaa agtgctgctg gttgggcagt caccaaatgc tgaactttcg gctcgcgcca
     5041 aacagattgc tatgggcgta gacggtgcca acgaagtgta taacgagatt cgtcagggcc
     5101 agccgattgg tctgggcgaa gcatctaacg atacgtggat caccaccaaa gtgcgttcgc
     5161 agctcttaac cagcgacctg gtgaaatcgt ccaacgtgaa agtgaccacc gaaaacggtg
     5221 aagtgttcct gatggggctg gtgactgaac gtgaagcgaa agcggcggca gatattgcca
     5281 gccgggtgag cggcgtaaag cgggtaacca cagcgtttac gtttattaaa tagcagcccc
     5341 ttgtaatgcc tgatgcgacg cttgccgcgt cttatcaggc ctacaacgat gcaaaccgta
     5401 ggtcggataa ggcgtttacg ccgcatccga catgttttcc ctcaaatcag aacaacagcg
     5461 ccagcccggc caccagacaa atcgccgcaa aaccccagcc gaggacaaac cccataaaca
     5521 ccagcgtcgc cgggtttaac accggattgc ccatccagaa tgccagcgca ccgcccatcg
     5581 atacctgttg gcgacgcatc cccgccgcga ccggagccgc acagcaggta cacatcatgc
     5641 ccggcagcga aaacagcgtt cccagcagcg tgccgcgaaa gcgcgattgc ccaagcgtac
     5701 gcaacaacca gtcacgcggg atcagcacct gaatcaacga accgaggatc acccccagca
     5761 ccgccgcttt ccatactgcg aggaaataga tcatcgcgta atccaacgcc gcttgccacg
     5821 gattagcatc cgcctgcgca aggatagatt taccgatact gtgggtttcg gctgcagtaa
     5881 acgctttgcc gtagtaatgt tgccatttca cataccagag accggcaatg acaacgagaa
     5941 agaaaagcgc gggtttccac cactgaatgg gtgttgccgc ctgagatgaa gactgaccag
     6001 tcatagcatt ccctaggaat aagttatgat aattgagcgc gtgaatatta cgctcactat
     6061 caattcttgg gaataattat tcagctcttt tgcgtaattc tgaagagctt aaaatcgtca
     6121 cgccttcatg ctcaggccgc atcgcctctg ccagcatgac ccgcgccacg tctctcgcat
     6181 caatggattt ccagttacct ggtaacaaac ggaacagcgg cgcaaaaagc gtttcgttca
     6241 tccgctgttt gctacgatcg cccagtaaca tcgacgggcg agcaatggtc aatttcggcc
     6301 agttctgggc aattaatgct tcttccatct cccctttgac gcggttatag aaaaacggcg
     6361 agtgggcatt ggcccccata gcactgacca ccagcatatg ctgcgcgccc agccgccgcc
     6421 cggttaatgc ggtatccact accagcgtgt aatcggcatg aataaacgcc tctttgctcc
     6481 ccgcttctcg ccgcgtggtg ccgagacaac aaaacacaat gtcgatgggt tcggtgacct
     6541 gcgccaacgc gtcactcagt tgcggatcat ggggattaaa caccccgggc atatcgccca
     6601 acggacgtcg cgtcggcgca gcaatggcgt taactttcgg ttcattaatc aacatccgca
     6661 gcaggtgacc gcccaccagc cccgttgcgc ctgtaatcag tacctgactc atcttcgctc
     6721 ctttacagaa ttgtctgcct tgcgctccac ggctcatgca ccagtcttaa tagaccatga
     6781 ggtaattatc cccgattgtg ggaaattcgc ctcatccaat gcaacaacgc ggaggaagca
     6841 tgagtaagaa aattgccgtt ttaatcagtg atgaatttga ggattcagaa tttacttcac
     6901 ccgcagacga gttccgtaaa gccggacacg aagtaattac cattgaaaag caagcgggta
     6961 aaacggtgaa aggcaaaaaa ggagaagcca gcgtgaccat cgataaatcc atcgatgaag
     7021 tgacgccagc ggagtttgat gccctgctgc taccgggcgg ccattcaccg gattatctgc
     7081 gtggggacaa ccgttttgtc acctttaccc gtgattttgt gaatagcggc aaacctgttt
     7141 tcgccatctg tcacggtccg cagctgctga tcagcgccga tgtgattcgg gggcgcaaac
     7201 tgaccgcagt taaaccgatc attattgatg ttaaaaatgc gggcgcggaa ttttacgatc
     7261 aggaagtcgt ggttgataaa gatcagctgg ttaccagccg gacaccggac gatctgccag
     7321 cgtttaaccg cgaggcgtta cgcctgctcg gtgcctgagt cgcgtagcca aatcattttt
     7381 ttaccaaagc ccagcgtgtt gtcggtgaat ttgatttcat cgagccggat ttcccacacc
     7441 ggggccgaca gcattctggc aaccggaaag cgatgattgt acgccttgcg cgcgaggtcg
     7501 ctttcctcac cttccagcct gcggatctca cctttaaact gcacaccgcg aattaacgct
     7561 accgttttcg gctgaccgtt taccgttccg gcaaccgcag cctgcggccc actcatctgc
     7621 gcgtggcgcg ttttttcttc cgtcaaaatg tagaaggcga ctttctgcgc atcaaacaga
     7681 taaaaagcat tagcgcacca cagttcccct tcctgctgca cacaccaggt gacaacatgt
     7741 tgttttgcca accaacggct gatggtgatg agtgtttcca ttgctgttct cttttatact
     7801 gtgggcctga ccttaacatg cgacgcagac gatgacaccc tggtttcttt acttgatccg
     7861 taccgccgac aataagcttt ataccgggat caccacggat gtcgaacgcc gctatcagca
     7921 gcaccaaagc ggcaaagggg cgaaagcact gcgcgggaaa ggagaactga cgctggcgtt
     7981 ttctgcgcca gtgggcgacc gttcgctggc actacgggcg gaatatcgcg ttaagcaact
     8041 gaccaaacgg caaaaagagc gtctggtagc ggaaggcgca gggtttgcag aactgttgag
     8101 cagcctgcaa accccggaga ttaaaagcga ttgaaatgct cgtgatactc aaccagaccg
     8161 gttacgccat tcagcgcgtc atccgctaaa cgatgtacct ggaaggcgct ttcagtgccc
     8221 ggccagcggc aacgcagatc gtgatgcgcc gccagttcaa agccgaaacg actgtacagc
     8281 gccggatcgc ccagcgtcac cactgcggca tagccgaact cattaagcga atcgagtcct
     8341 tcatagacca gttggcgtgc cagcccctgt ccacggtatt tttcatcaac cgccaacggt
     8401 gccatgccga cccattgcag gtcttcgccc tgcacatcaa ccggactaaa tgccacatag
     8461 ccaatgacct gaccttcgtc atctgtcgcc accagcccca gcgtcagaaa gccatcttca
     8521 cgcagatcgt gaaccagctt cgcttccgca tcactttcga atgagcgacg cagcagggca
     8581 tcaatacccg gcgcatcaat gggaatttct actcgaatta gcatggttca cctaccgatg
     8641 tctgtttggt ttcaggcgcg gttttcattc ccgcctcaac aaaatccgcc agttgcagca
     8701 gcatcatgcg taaggctttc ggcatttgct ccagttcaat ggcgtccatc aggtttttca
     8761 catgcagccc cagctccgta tcgccttcaa tcaccagccg acgctggaag aagagcgtat
     8821 ctggatcttg tttacgcgcc gcaatcatca gcagatcgct gacgtcggca ctaaaactca
     8881 catcagcttg cgcgttctgg ctgaccacca gtttgccatt caccaccgag gtaaaccatt
     8941 gcaggtcaat atcacgcaca tgaatactta accagcggcc ttcaagaaac tccagctcgc
     9001 catcatccag cgcctgacgg aattgccagc ttaagacctg ctcaagaacc tggcgtttta
     9061 gcgcaaatgg cgtcagtttt accggtacac tcaacagaga tggccccaaa tgcacaatac
     9121 gggaacgaag tttatccaac acgagtttta ctccctgttt caacaatcat cctattttgc
     9181 catatcagaa aaataacata gcggtataaa tcaacaattc catatgaaat tgctgctacc
     9241 accaatacaa ctttaactgc cttaaatcaa aaattgtcgc agcaaggtta actaaaatcc
     9301 cagttcgtta acatttttgc gttttgatag cgcaaccttc aggaaaaatt atggagctgc
     9361 tctgccctgc cggaaatctc ccggcgctta aggcggccat cgaaaacggc gcagatgctg
     9421 tttatatcgg gctaaaagat gataccaatg cccgtcactt cgccggcctt aactttaccg
     9481 agaaaaaatt gcaggaagcg gtgagttttg tccatcaaca tcgccgcaaa cttcacatcg
     9541 cgattaacac ttttgcgcat ccggacggtt acgcccgttg gcagcgcgcc gtggatatgg
     9601 cggcgcagct gggtgccgac gcgctgatcc tcgccgacct cgccatgctg gaatacgccg
     9661 ccgagcgtta tccgaatatt gagcgccacg tatcggtgca ggcttcggcg accaatgaag
     9721 aggcgattaa cttttatcat cgccattttg acgttgcccg cgtggtgctg ccgcgcgtgt
     9781 tgtcgattca tcaggtgaaa cagctggcac gggtcacacc tgtaccactg gaagtgtttg
     9841 ctttcggcag cctgtgcatt atgtcggaag gccgttgcta tctgtcgtcg tatctgacgg
     9901 gtgagtcgcc taacaccgtg ggcgcgtgtt ctccggcccg tttcgtgcgc tggcaacaaa
     9961 cgccgcaggg gctggaatcc cgcctgaacg aagtgctgat cgaccgttat caggacggcg
    10021 aaaacgcagg ttatccgacg ctgtgtaaag ggcgttatct ggtggacggc gagcgctatc
    10081 acgcgctgga agaaccaacc agtctcaata ccttggaact gctgccggag ttaatggcgg
    10141 cgaatattgc ttcggtgaaa attgaaggcc gccagcgtag cccggcgtat gtcagccagg
    10201 tggcgaaagt ctggcgtcag gctatcgacc gttgtaaggc cgatccgcaa agcgcgtgga
    10261 tggagacgct cgggtcgatg tccgaaggca cgcagaccac ccttggcgcg tatcaccgta
    10321 aatggcagtg agaagagcaa tgaaatattc cttagggcca gtgctgtggt actggccaaa
    10381 agagacgctg gaagattttt atcagcaggc cgccaccagc agcgccgacg tgatttatct
    10441 tggagaagcg gtatgcagca agcgccgggc aaccaaagtt ggcgactggc tggagatggc
    10501 aaaatcgctc gccggaagtg gcaagcagat tgtgctctcc acgctggcgc tggtgcaggc
    10561 atcctctgaa ctgggcgaac tgaaacgcta tgttgagaac ggtgagtttc tgattgaagc
    10621 cagcgatctc ggcgtggtga atatgtgcgc tgaacgcaaa ctgccgttcg tcgccgggca
    10681 cgcgctgaac tgctacaacg cggtgacgct gaaaatattg ctcaaacagg gcatgatgcg
    10741 ctggtgtatg ccggtggagc tttcccgcga ctggctggtg aatctgctta atcagtgcga
    10801 tgagctgggc attcgcaacc agtttgaagt ggaagtcctg agctacggtc atctaccgct
    10861 ggcctactcc gctcgctgct ttaccgcgcg ttcggaagac cgcccgaaag atgagtgtga
    10921 aacctgctgc attaagtatc cgaacgggcg caacgtgctg tcgcaggaaa accaacaagt
    10981 gtttgtactc aatggcattc agaccatgag cggctacgtt tacaacctcg gtaacgagct
    11041 gacatccatg cagggattgg ttgatgtggt ccgcctgtca ccgcagggta ctgacacttt
    11101 cgcgatgctc gacgccttcc gcgctaatga aaatggcgct gcgccactat tgttgacggc
    11161 aaacagcgac tgtaacggct actggcggcg gctggcagga ctggagctgc aagcctaagt
    11221 aaatatgctc gtcatacttc aagttgcatg tgctgcatct gcattcgttc accccagtca
    11281 cttacttatg taagctcctg gggattcact ctcttgtcgc cttcctgcaa ctcgaattat
    11341 ttagagcaca gctcactttg ttaacaactt taactactct ttaatgcagt attaaagatt
    11401 aatcggtaac aaagtgagct gttatgactg ataaaaccat tgcgttttcg ttactcgatc
    11461 tggcccccat tcccgaaggt tcttcagcgc gagaagcatt ctcccactct ctcgatctcg
    11521 cccgtctggc tgaaaagcgc ggctatcatc gctactggct ggcagaacac cacaatatga
    11581 ctggcattgc cagtgctgcc acgtcggtat tgattggcta tctggcggcg aataccacca
    11641 cgctgcatct ggggtctggc ggcgtgatgt tgcctaacca ctcaccgttg gtcattgccg
    11701 aacagttcgg cacgctcaat acactctatc cggggcgaat cgatttgggg ctgggtcgtg
    11761 cgccaggtag tgaccaacgg acaatgatgg cgctgcgtcg ccatatgagc ggcgatattg
    11821 ataatttccc ccgcgatgtc gcggagctgg tggactggtt tgacgcccgc gatcccaatc
    11881 cgcatgtgcg cccggtacca ggctatggcg agaaaatccc cgtgtggttg ttaggctcca
    11941 gcctttacag cgcgcaactg gcggcgcagc ttggtctgcc gttcgcgttt gcctcacact
    12001 tcgcgccgga tatgttgttc caggcgctgc atctttatcg cagcaacttc aaaccgtcgg
    12061 cacgactgga aaaaccatac gcgatggtgt gcatcaatat tatcgccgcc gacagcaacc
    12121 gcgatgccga attcctgttt acctcaatgc agcaagcctt tgtgaagctg cgccgcggcg
    12181 aaaccgggca actgccgcca ccgattcaaa atatggatca gttctggtcg ccgtccgaac
    12241 agtatggtgt gcagcaggcg ctgagtatgt cgttggtagg tgataaagcg aaagtgcgtc
    12301 atggcttgca gtcgatcctg cgcgaaaccg acgccgatga gattatggtc aacgggcaga
    12361 ttttcgacca ccaggcgcgg ctgcattcgt ttgagctggc gatggatgtt aaggaagagt
    12421 tgttgggata gtgtgtctta acgcgggaag ccttatccga gctggcaacg ctgtcctacg
    12481 tagacctgat aagcgaagcg catcaggcat tgtgtaggca gcagaaatgt cggataaggc
    12541 accgctgatt actgatacac cggcagtaaa ttaaagctcg ataaaatatg taccaatgcg
    12601 ttgccgacgc caaacaccag aatcagcgta atcatcggct tgccgcccca gacgcggaat
    12661 ttcgggctgc caaagcgttt acgcgatgcg cgggccagca gtgccggaac aattgccgcc
    12721 cagatggtag ccgctaaacc agcataacca atggcgtaca ggaatccatt cgggaacagc
    12781 agaccaccca caacaggcgg tgcaaaggtc aacaatgctg ttttcaagcg gcccatagcc
    12841 gagtcatcga aaccaaacag atctgccaga tagtcaaaca aacccagcgt tacgccaagg
    12901 aacgaactcg ctaccgcaaa gtttgagaac acgaccagca gaaggtccag gctacggctg
    12961 ttcagcacgc cgcttaatgc ctgtaccagc acatcaatat taccgccctt ctctgcaata
    13021 ccgataaact ccggacgcgg gatgttaccc atcgtcgcca gcaaccagat ggtatacagc
    13081 gccagtgcca tcagcgtacc gtacaccagg catttcacga tggttttcgg atctttgccg
    13141 taatacttca tcaggcttgg cacgttaccg tgataaccaa acgacgccag gcagaacggc
    13201 aatgtcatca gcagatacgg cgcataagat gcattgcttt ctgcaacgtt gaacaatgtc
    13261 gcaggctgca catgccccaa caggctgcca aaggtgagga agaaggtaat gactttcgcc
    13321 cccagcacaa tcgccgtcat gcggctgacg gccttagtac tcaaccacac cacaaacgct
    13381 accagcagtg caaaaccaaa acccgccgcc cgtgccggga cgtttagcga catctctgcg
    13441 aaggtgtgat gcagaattga accactggca gaaatatagg cataggtcag gatatagagc
    13501 acaaaggcaa tggaaatacc gttgaccacg ttccagcctt tgcccagcaa atctttggtg
    13561 atggtgtcaa aactcgaacc gattcggtaa ttcaggttag cttccagaat catcaagccg
    13621 gaatgcagca tacagaacca ggtaaagatc agcgccgcca ttgaccagaa aaaccacgcc
    13681 ccggacatga ccactggcag agaaaacatc cctgcgccaa taatggtgcc gccgataatc
    13741 accacgccgc caagcagcga cggtgacgtt tgggtggtgg ttagtgttgc catgagggct
    13801 tctctccagt gaaaaatagt gcgactgcgt tgttatgcat tgcactgtac cagtacgcga
    13861 gtacaaaaga cagaaaaaaa gccccgatgg taaaaatcgg ggctgtatat attattttac
    13921 agattgtgtt cgctgttcag cgatgattac gcatcaccac cgaaacgacg acgaccggta
    13981 gagtcatcac gacgcggagc gcggccttca cgacgttcgc cgctaaaacg acgaccatca
    14041 ccacggccac cttcacggcg ttcaccgctg aagttacgac cgccttcacg acgttcgcca
    14101 ccgaaaccac gaccaccgcc acgacgctca ccgccagtat gcggctgtgc atcgcccagt
    14161 aactgcatgt tcatcggctt gttgagaatg cgagtgcgcg taaagtgttg cagcacttca
    14221 cccggcatac ctttcggcag ttcgatggtg gagtgagaag caaacagctt gatgttacca
    14281 atgtaacggc tgctgatgtc gccttcgtta gcaatcgcac caacgatatg acgaacttca
    14341 acaccatcat cacggcccac ttcaatgcgg tacagctgca tatcgccaac atcacgacgt
    14401 tcacgacgcg gacgatcttc acggtcacca cgcgggccac ggtcgttacg atcgcgcgga
    14461 ccacggtcat cacggtcacg gaattcacgt ttcggacgca tcggcgcatc tggcggtacg
    14521 atcagagtac gttcaccctg tgccattttc agcagtgccg cagccagagt ttcgagatcc
    14581 agctcttcac cttctgcagt cggctgaatt ttgctcagca gtgcgcggta ttgatccaga
    14641 tcgctgcttt ccagctgctg ctgtacttta gcggcgaatt tttccagacg gcgtttgccc
    14701 agcagttctg cgttcggcag ttctacttcc ggaatagtca gcttcatagt acgttcaatg
    14761 ttgcgcagca gacgacgctc gcggttctca acgaacagca gcgcgcggcc agcacgaccc
    14821 gcacgaccgg tacgaccgat acggtgaacg taagactcag aatccatcgg gatatcgtag
    14881 ttaactacca ggctgatacg ctcaacgtcc aggccacggg ctgcaacgtc ggtcgcaatc
    14941 aggatgtcca gacgaccatc tttcaggcgt tccagtgtct gttcacgcag cgcctggttc
    15001 atgtcaccgt tcagcgcggc gctgttgtag ccgttacgct caagagcttc agccacttcc
    15061 agagtcgcgt ttttggtacg aacgaagata atcgccgcat caaaatcttc cgcttccagg
    15121 aaacgtacca gtgcttcgtt tttgcgcata ccccagacag tccagtagct ctggctgatg
    15181 tcaggacggg tagtcacgct ggactgaatg cgcacttcct gcggctcttt cataaagcgg
    15241 cgggtaatgc gacgaatcgc ttccggcatg gttgcagaga acagagcggt ctgatgacct
    15301 tccgggatct gcgccataat ggtttcaacg tcttcgatga agcccatgcg cagcatttcg
    15361 tcagcttcat ccagaaccag accgctcagt ttagagaggt ccagagtgcc acgtttcagg
    15421 tggtccagca gacggcccgg agtaccgaca acgatctgcg gcccctgacg cagggcgcgt
    15481 aattgcacgt cataacgctg gccgccgtac agagcaacca catttacgcc gcgcatgtgt
    15541 ttagagaaat ccgtcattgc ttcagcaacc tgtaccgcca gttcgcgggt cggtgccagc
    15601 accagaatct gtggtgcttt cagctcagga tcaagattct gcaacagagg taaagagaat
    15661 gctgcagttt ttccgctccc cgtctgggcc atacccagaa cgtcgcggcc attcagcaga
    15721 tgtggaatac actctgcctg aattggagat ggtttttcgt aacccagatc gttaagggct
    15781 tcaaggatag gagccttcag gcccagatct gcaaaagtgg tttcgaattc agccatgtag
    15841 tacgtgtgcc tcaaaattaa tggcggccag tctacataac tcatcatgaa attgatcagc
    15901 aattttcatt gaaaagtgtg aaccggctca aagtaggtgt attaacgaac aacaacgccc
    15961 tcacccctta aggtgatggc aatcaaaaaa gattacgggc tgatgtgtac gtcagctatt
    16021 gctggtccga ttctgccagg tcatcttggt cctggcccag gagcgataat tccaacaatg
    16081 cgtatcggtg ctcaacaaag ttatgaacgt tgttggcaac cgccagtttg aacagtgccg
    16141 tggcgctgtc cgaatccccc agacttaggt agtacttacc taaatagaag ttggtttcac
    16201 tgagatgctc agcgagcgag gtgttatccg ttgcgtccgc cttgagcctt tccattaacg
    16261 tttgttcgct aatgttgccc aggtagaact cgacaatgtt ccatccccac tgttccttat
    16321 ccgatttttc gaagtgctgt ttcaacactt ctttagcctg cttctcatcg agcttctgct
    16381 cggcgagata aagccacaga ctacggaaag gatcattggg atcgtcttga taaaacgcca
    16441 gcagatcatc ttgcgctaac ttgtcacgac cgccgtaata taatgcgatc ccgcgattca
    16501 agtgcgcgta gttgtaagtt ggatcaagct caagtacaga atcaaacgct tcataggcag
    16561 catcaaaatt gcctgcctgc gttaaatata tgcctaagta attgaacact tcaggcatat
    16621 ccggtcggat tgccagcgct tgcgaaaaat cgttacgcgc taatgccctc agaccgagac
    16681 tatcatacaa cactccgcgc tcatataaaa gctgtgcgcg ttcgtcatcg gttaaagccc
    16741 gactggcaag gatttgttcc atacgtgcca gaatcacttc ctgctgtaaa gtcggttgca
    16801 atggtaccgc gaggacttca cttttacgcc aggaagtatt actgcatcct gcaagcgtaa
    16861 gtgctgtcgc aacgaaacac cagcgcaaaa aaggcttcat ttcccactcc cgaagaccac
    16921 ggttgaatga acgtcctgtt cccggttgct aacaaggcgt cctgcccggt taaaagcccc
    16981 ccgccgcagc ggagggcaaa tggcaacctt actcgccctg ttcagcagcc ggagcttccg
    17041 gtgctgcagc aggttgagac tgctcagtcg cttctttaat gctcagacgg atacggccct
    17101 ggcgatcaac ttccagaact ttcaccggta cttcctgacc catctgcagg taatcggtca
    17161 ctttctcaac gcgtttgtca gcgatttgag agatgtggac cagaccttct ttaccgccgc
    17221 cgatggcaac aaatgcgcca aagtcaacga tacgggtcac tttaccagtg tagacgcggc
    17281 ccacttcgat ttctgcagtg atctcttcga tacgacgaat agcatgtttc gctttctcgc
    17341 cgtcggtcgc tgcgatcttc acagtaccgt catcttcgat ttcgatggta gtgccagttt
    17401 cttcggtcag ggcacggata acagagccgc ctttaccgat aacgtctttg atcttgtccg
    17461 ggttgatctt gatggtatgg atacgcggtg cgaactcaga gatatcgcca cgcggcgcgt
    17521 tgatcgcctg ttccattacg cccaggatat gcagacgcgc acctttagcc tggttcagcg
    17581 caacctgcat gatctctttg gtgatacctt caattttgat atccatctgc aatgcagaga
    17641 taccctcgcg ggaacccgca actttgaagt ccatatcgcc caggtgatct tcgtcgccca
    17701 aaatgtcaga cagtacaacg tagttgtcgc cttctttcac cagacccatt gcgatacccg
    17761 caacggcagc tttgatcggc acacctgcgt ccatcagcgc cagagacgcg ccgcacacgg
    17821 aagccataga agaggaaccg ttggattcag tgatttcaga cacaacacgt acggtgtacg
    17881 ggaatttgtc catatccggc atgactgcca gcacgctgcg cttcgccaga cgaccgtgac
    17941 caatttcacg acgcttcgga gaaccgacca tgccggtttc gcctacggag tacggaggga
    18001 agttgtagtg gaacaggaag gtatcggtac gttcgcccat cagttcatca agaacctgcg
    18061 cgtcacgagc agtacccagc gttgcggtaa ccagcgcctg cgtttcacca cgggtgaaca
    18121 gcgcagaacc gtgagtacgc ggcagcacgc cagtacgcac atccagacca cggatcatat
    18181 ctttttcacg accgtcgata cgcggttcgc ctgccagtac gcggctacga acaacgtttt
    18241 tctcgatagc gtgcagaatt tcacccagtt cgttttcgtc cagggtttcg tcttcagcaa
    18301 gcagcgtcgc gatggtttca gatttgatga catcaacctg cgcataacgc tcttgtttgt
    18361 cggtgatgcg gtaagcatcg ctcaggcgag cttcagccag tgcagcaacg cgcgcgttca
    18421 gcgcttcgtt taccggctcc ggctgccagt cccaacgtgg tttaccggct tctttcacca
    18481 gttcattgat gttctgaata acaacctgct gttgttcatg accgaacact actgcgccca
    18541 gcatctggtc ttcgctcagc agttcagctt cagattcaac catcagaaca gcggcttcag
    18601 taccggcaac aaccagatcc agtttgctct ctttcagctc gtcctgagtc gggttcagta
    18661 cgtactggtc attgatgtaa cctacgcgag cagcaccaat cggaccattg aacggaatac
    18721 cagacagaga cagtgctgcg gaagcaccaa tcatcgcgac gatatccggg ttaacttgcg
    18781 ggttaacaga aaccacggtg gcgataacct gaacttcgtt gacgaagcct tccgggaaca
    18841 gcgggcgaat cgggcggtca atcagacgcg cgatcagggt ttcgccttcg cttggacgac
    18901 cttcacgacg gaagaagcta cccgggatac gaccagcagc gtaggtacgc tcctgatagt
    18961 taacggtcag tgggaagaag tcctgacctg gtttggcttt tttctggcca acaacggtaa
    19021 caaataccgc ggtgtcatcc atgctaacca taacagcggc agtagcctga cgagccatca
    19081 tgccggtttc cagagtcacg gtgtgttggc cgtactggaa tttacgaacg atcggattaa
    19141 gcaatgtaat atcctttctc tttcttagac agtaccttac ggcactggtg ttaatacccg
    19201 atcttctgcg catcctcgcg actaatgaca accctaaccc agctctatgt gggtaaagcc
    19261 tctcattagc cgcgcgaacc tctgcaacgg aagatcattc atagcaacaa tacattagtt
    19321 tccagtgaat tgctgccgtc agcttgaaaa aaggggccac tcaggccccc ttttctgaaa
    19381 ctcggcagga attagcgacg cagacccagg cgctcgatga gctgggtgta acgtgctacg
    19441 tctttacgtt tcaggtagtc gagcagttta cgacgctgag aaaccatgcg cagcagacca
    19501 cgacggctgt ggtgatcttt tttgtgctct gcaaagtggc cctgcaggtg gttgatctgt
    19561 gcagtcagca gtgctacctg aacttcggta gaaccggtgt cgtttgcgtc acgaccaaac
    19621 tcagaaacga ttttagctgt tgcttcagta cttagagaca ttttaaaact ccaaagtata
    19681 tagaatgaaa ggacgccgat ctctaattca gcgatcccag tgtacgttac gcaaagtgtt
    19741 aaacaattta cgcgacgtta agcggcagta ttctactcgt agcgacttgt tatcgcaaga
    19801 cggttaacat tacgccgggt attcaaccac caggcgacga ggcgcgacgc ggccttcatc
    19861 gtcaatttcg cccataccga taaacttgcc gttctcacct tctgtaacgc gaaccagtcc
    19921 ttccagcggc gcaccggatg tacgaactgg gttaccattt ttgaagtaaa cagaagacgt
    19981 taacggaaga ttcaccaccg ggtagtccga agctggactg tccattggca tcagtaatgg
    20041 atcaagtaac tccgcagccg gaatatcctg ctgttcggct tgttcgacca gttcacgcag
    20101 atgttccagg gtcaccatcc gttcaaccgg atatttactt accgccagac gacgcaggta
    20161 aataacatgc gcgccacaac cgagtttttc acccaggtca tcaatgatgg tgcggatata
    20221 agtgcctttt gagcagtgaa tttccagctc cagctcattg ccttcgtggc gaataaacag
    20281 caattcataa atggtaatcg ggcgcgcttc acgcggtact tcaatgccct gacgcgcata
    20341 ttcgtacagt tttttaccct gatatttcag tgctgaatac atcgaaggga tctgttcgat
    20401 atcgccacgg aaagtatcca gcgccgccgc cagttgctct gcgctaaagg ttaccggacg
    20461 ttcttcaacg atttgtccgt cggcatcaga agtatcggta cgctgtccaa gacgcgcaat
    20521 gacccgatag cgtttgtcgg agtccagcag atactgggaa aacttcgtcg cttccccgaa
    20581 gcaaattggc aacatgccag ttgccagcgg gtccagcgca ccggtatgcc cggcacggtt
    20641 ggcgttataa atccgtttca ctttttgcag cgcatcattg ctggacatac cctgcggttt
    20701 atccagcaac aaaacgccgt taatgtcgcg accgcgacga cgaggacgac tcattagtcc
    20761 tccttgctgt cgtccgggtt aacacgacgt tcttcgtcat gtttgaccac gctggtcacc
    20821 aggtttgaca tgcgcatccc ttcaaccaga gagttgtcgt agaagaaggt cagttccggc
    20881 acgatacgca ggcgcatcgc tttccccagc aggctgcgga tgaaaccaga agcttcttgc
    20941 aacgctttga tgcccgcttt aaccgcgtct tcatctttgt cgttgaggaa cgtcacatat
    21001 actttggcat acgccaggtc gcgagacatt tcgacaccgg aaacggtggt catcatgccc
    21061 aggcgaggat ctttaatttc acgctgcagg atgagagcga tctctttttg catttcctgc
    21121 gctacgcgct gcgggcgacc aaattctttc gccataataa attctcctga caaaaaaggg
    21181 actgttagcc cctttttaaa attaatttca ggtggaaggg ctgttcacgt tggcctgata
    21241 agacgcgcca gcgtcacatc aggcaatcca tgccggatgc ggcgtaaacg ccttatcccg
    21301 catggaaccc taaaaacctt aagcgatggt acgttggatc tcgatgattt cgaatacttc
    21361 gatcacatcg ccagtgcgga cgtcgttgta gttcttaacg ccgataccac attccatacc
    21421 gttacggact tcgttaacgt catctttgaa gcggcgcagg gactccagct cgccttcgta
    21481 gataaccacg ttgtcacgca gaacgcggat cgggttgtga cgtttaacca caccttcggt
    21541 aaccatacag cctgcgatgg caccaaattt cggcgatttg aacacgtcac gaacttccgc
    21601 cagaccgata atctgctgtt tcagttccgg agacagcata ccgctcatcg ccgctttcac
    21661 ttcgtcaatc aggttataga tgacggagta gtaacgcaga tctaggcttt ccgcttcaat
    21721 cactttacgt gcagaggcat cagcacgtac gttgaagcca accaggatgg cgttggacgc
    21781 cgcagccagg gtggcgtcgg tttcggtgat accacctacg ccagaaccga tgatcttcac
    21841 tttaacttcg tcagtagaca gtttcagcaa ggagtcggag atcgcttcca cagaaccctg
    21901 tacgtctgcc ttcaggacga tgttcacttc gtgaacttcg ccttcggtca tgttggcgaa
    21961 catgttctcg agtttagatt tctgctgacg cgccagttta acttcgcgga atttaccctg
    22021 acgatacagt gcaacttcac gcgctttctt ctcgtcacgt acaacggtaa cttcatcacc
    22081 cgcagccggt acgccggaca ggccgaggat ttccaccgga atggacggac ccgcttccag
    22141 cacttcctga cccagttcgt tacgcatcgc acgaacacga ccgtattcga agccacacag
    22201 aacgatatcg cccttgtgca gagtaccttc acgtaccaga acggtagcaa ccggaccacg
    22261 acctttatcg aggaaggatt cgataaccgc accgctcgcc atacctttac gtaccgcttt
    22321 cagctccaga acttccgcct gcagcaggat agcgtccagc agttcatcga tgccggtacc
    22381 cgctttcgca gatacgtgta cgaactggct ttcaccgccc cactcttctg gcaggatgcc
    22441 atactgggag agttcgttct taacacgatc cggatcggct tctggtttat caatcttgtt
    22501 cactgcaacc accaccggta cctgcgccgc tttcgcgtgc tggattgctt cgatggtctg
    22561 cggcatcacg ccgtcgtcgg cagcaacaac caggactacg atgtccgttg cctgcgcacc
    22621 acgagcacgc attgaagtaa acgcggcgtg ccctggggtg tccaggaagg tgatcatgcc
    22681 gttttcagtt tcaacgtggt atgcaccaat gtgctgggta atgccgcccg cttcgccaga
    22741 ggccactttc gttgaacgaa tgtagtccag cagagaggtt ttaccgtggt caacgtgacc
    22801 catgatggtc acaaccggcg cgcgcggttc agccgcagcc cccgtgtcac ggtcgctcat
    22861 taccgcctct tccagctcgt tttcacgacg caggataact ttgtggccca tctcttcagc
    22921 aaccagctgt gcggtttcct gatcgataac ctggttgatg gttgccattg cgcccagttt
    22981 catcatcgct ttgatgacct gagagccttt aaccgccatc ttgttcgcca gttcgccaac
    23041 ggtgatggtt tcgccgatca caacgtcacg gttaacggcc tgagctggct tctggaagcc
    23101 ttgctgcagg gaagaacctt tacgttttcc gcctttacct ccacgtactg ctgcgcgagc
    23161 ttcttcacga tcagcttttg attcagcgtg tttgttgcct ttcttcggac gcgctgcttt
    23221 cgcgttacga ccacggccac ggccgccttc gacttcacga tcgctttcgt cttctgcctg
    23281 gcgagcatgt tgagaagtag tgacgtgata atcgctggaa tcttcagtcg gttccgcgtt
    23341 atcagtccat ttgttttctt ccgccatacg acgtgcttct tcagcaacgc gacgtgcttc
    23401 ttcttcgagt ttacgacgcg cttcttcttc agctttacgc ttgagctctg cagcttcctg
    23461 ctcacggcgg gctttttcag cctgggcgtt tttagtcata tcgtcttgtt gattgctcac
    23521 tttgtctttt tccgcagctt cacgtttcgc ttgttcagca gcttcacgct tagcttgttc
    23581 tgcggcctca cgttcagctt tttgttgcgc ctcgcgttta gccgattctt ctgcctcacg
    23641 acgggcttgc tcttccgctt cacgctgcgc ttgctcttcc gcttcacgct gcgcttgctc
    23701 ttccgctgca aggcgttcag cctcttgcgg atcgcgtttc acaaaggtgc gtttcttgcg
    23761 gacttcgatt tgtaccgatt tgctttttcc accggtacca ggaatgttaa gggtgctgcg
    23821 tgttttacgt tgcagcgtca atttgtccgg gcctgaattt ttctgattca ggtggtcaat
    23881 caaagtctgt ttctcttgtg cggacacaga gtcgtcagca gacttccgga tacctgcatc
    23941 agcaaattgc tgtaccaggc gttccacgga ggtctgtcgc tctgcggcca gcgttttaat
    24001 cgttacatct gtcatgctgt tccttcctgc tacagtttat tacgcttcgt caccgaacca
    24061 gcaaatatta cgggcagcca taatcagtgc tccggctttt tcgtcggtca ccccttcgat
    24121 atcagccaga tcatcaatgc cctgttcggc gagatcttcc agcgtacaaa cgccacgggc
    24181 ggccagtttg aatgccaaat cacgatctat cccttcaagg ttcagcagat cgtcagccgg
    24241 tttgttatca ccgaggcttt cttcctgggc ctgtgcaatg gtggccagtg catttttagc
    24301 acgctcgcgc agtgcttcaa cggtcggctc atcaaggcct tcgatttcca acagctcttt
    24361 catcggcaca taggccagtt cttccagcgt cgagaagcct tcttctacca gaacagtcgc
    24421 gaagtcttcg tcgatgtcga gatatttggt gaaggtgtcg atcgctgcgt gcgcttccgc
    24481 ctgatgctta gcctgcaggt cgtcaacggt catcacgttg agttcccaac cgctcagctg
    24541 cgaagccaga cgcacgttct gaccgttacg gccaatcgcc tgcgccaggt taccggcttc
    24601 aacggcgata tccatggtgt gtttatcttc atccaccacg atagaagcaa cgtctgccgg
    24661 tgccattgcg ttaatcacaa actgcgccgg gttatcatcc cacaggacga tatcgatacg
    24721 ctcgccgccc agttcagtag aaaccgcctg aacacgcgcg ccacgcatac ctacacaggc
    24781 acctaccgga tcgatacgtt tatcgttggt tttcaccgcg attttcgcac gagaacccgg
    24841 atcgcgagcc gctgctttaa tttcaatcac ttcttcgccg atttctggca cttcaatacg
    24901 gaacagttcg atcagcattt ccggcttgga acgagtgacg aacagttgcg cgccacgcgc
    24961 ttccgggcga acggaataga gcacgccacg aacgcggtcg ccagggcgga agttttcacg
    25021 cggcagcata tcttcgcgca ggatcacggc ttcagcattg ttgcccagct ccagagagat
    25081 gttgtcgcgg tttacttttt tcaccacgcc ggtgatgatt tcaccttcgt gttcacggaa
    25141 ctgatcaacc accatcgcac gttcggcttc acgcactttc tgcacgataa cctgttttgc
    25201 cgtctgggta gtgatacggt caaaggtaac agactcaatc tgatcttcaa cgtaatcgcc
    25261 caggttcagg ctttcatctt cataacgtgc ggcttcaagg gtgatttcct tggtcggctg
    25321 ggtgacttca tcaacaacta accagcgacg gaaggtgtca aaatcaccgc ttttgcgatc
    25381 gatctgtacg cggacgtcga tctcttgttc atattttttc tttgttgctg tcgccagcgc
    25441 gctttccaat gcttcgaaaa tcttctcgcg aggtagcgcc ttttcattgg atacggcttc
    25501 aactacagcc aaaatttctt tgttcatcgc gggcttttca cctcatccag actattaaaa
    25561 gtggggaacc aggttcgcct tctggatatt actcagcgcg aacacttcat ctttaccttc
    25621 gacggtaact gtgatcattt caccgtctac cgctttgata acgccctgcc atttacgacg
    25681 gttttgtacc gccatacgga gaaccagagt cacctcttct ccgacaaaac gggcgtagtg
    25741 ttcagccgtg aacagtgggc gatcgagacc cggtgaggag acttccaggt tataagcaac
    25801 ggtgatggga tcttcaacat ccagcacagc acttacctgg tggctcacat cagcacaatc
    25861 atcaacattg atgccatctt cactatcaat atagatgcgc agtgtggatg tgtgaccgcg
    25921 aataaattcg atgccaacca gttcaaaacc cagggcctca actggcgcag taatcatctc
    25981 tgttaatttt tgctctaatg tggacaagcc cacccccaag acataaaaaa agggcctaaa
    26041 gcccagttat tctgtagtca gataacaaaa aaccccgata aatcggggct ttatataact
    26101 gaaccctata accgcaactg cggtctggag cactttccag aaggattttt tcaaatccca
    26161 ctacgaaggc cgaagtcttc acagtatatt tgaaaaagga ctctaaggga aagtggttgc
    26221 gggggccgga tttgaaccga cgaccttcgg gttatgagcc cgacgagcta ccaggctgct
    26281 ccaccccgcg cctgaaactc ggcaaattct actcgttttg ggtaaaagat gcaaatactg
    26341 ctgggatttg gtgtaccgag acgggacgta aaatctgcag gcattatagt gatccatgcc
    26401 acattttgtc aacgtttatt gctaatcacg tgaatgaata tccagttcac tttcatttgt
    26461 tgaatacttt tgccttctcc tgctctccct taagcgcatt attttacaaa aaacacacta
    26521 aactcttcct gtctccgata aaagatgatt aaatgaaaac tcatttattt tgcataaaaa
    26581 ttcagtgaga gcggaaatcc aggctcatca tcagttaatt aagcagggtg ttattttatg
    26641 acgacgattc tcaagcatct cccggtaggt caacgtattg gtatcgcttt ttctggcggt
    26701 ctggacacca gtgccgcact gctgtggatg cgacaaaagg gagcggttcc ttatgcatat
    26761 actgcaaacc taggccagcc agacgaagag gattatgatg cgatccctcg tcgtgccatg
    26821 gaatacggcg cggagaacgc acgtctgatc gactgccgca aacaactggt ggccgaaggt
    26881 attgccgcta ttcagtgtgg cgcatttcat aacaccaccg gcggcctgac ctatttcaac
    26941 acgacgccgc tgggccgcgc cgtgactggt accatgctgg ttgctgcgat gaaagaagat
    27001 ggcgtgaata tctggggtga cggtagcacc tataaaggaa acgatatcga acgtttctat
    27061 cgttatggtc tgctgaccaa tgctgaactg cagatttaca aaccgtggct tgatactgac
    27121 tttattgatg aactgggcgg ccgtcatgag atgtctgaat ttatgattgc ctgcggtttc
    27181 gactacaaaa tgtctgtcga aaaagcctac tccacagact ccaacatgct tggtgcaacg
    27241 catgaagcga aggatctgga atacctcaac tccagcgtca aaatcgtcaa cccgattatg
    27301 ggcgtgaaat tctgggatga gagcgtgaag atcccggcag aagaagtcac agtacgcttt
    27361 gagcaaggtc atccggtggc gctgaacggt aaaaccttta gcaacgacgt agaaatgatg
    27421 ctggaagcta accgcatcgg cggtcgtcac ggcctgggca tgagcgacca gattgagaac
    27481 cgtatcatcg aagcgaaaag ccgtggtatt tacgaagctc cggggatggc actgctgcac
    27541 attgcgtatg aacgtctgtt gaccggtatt cacaacgaag acaccattga gcagtatcac
    27601 gcgcatggtc gtcagttggg ccgtctactg taccaggggc gttggtttga ctcccaggcg
    27661 ctgatgctgc gtgactctct gcaacgctgg gttgccagcc agatcactgg tgaagttacc
    27721 ctggagctgc gccgtgggaa cgattattca atcctgaata ccgtctcaga gaacctgacc
    27781 tacaagccag agcgtctgac gatggaaaaa ggcgactcgg tgttctcgcc agatgatcgt
    27841 attggtcaat tgaccatgcg taatctggat atcactgata cccgagagaa actttttggt
    27901 tatgccaaaa ctggcctgct ctcctcctct gccacttcag gcgtgccgca ggtggagaat
    27961 atggaaaaca aaggccagta attcgacatc aaccctgccc ttcagggggg gggttacctt
    28021 gcttatcctc tgtaaactcc tttctgaaca tcacataatc gaaaacatca tggtttgcat
    28081 ccacaacccg tgagtctcct ttatagtgtg caatcacttc ctcaagcgtt tgcggctgtt
    28141 ccggctttgt tacccccatc cacgcattaa taaggtaatt attgtaagct gtcgaaaaga
    28201 tgttgttttc cgttgtgcga tccacgccat ccccaagaac ggggctatac cagataaaca
    28261 tcgggacatg atatgcctgc tggctagcct ccctgcctcc atgaaaatag acgttcttct
    28321 tcgtagggtc acgttccagg ccgtggtcgg caaaatacat gaccgaggcg cggcgatctt
    28381 ttaataattc aaaaacctga cctagcaaac tatctgtgta atgaatggag ttgtcatagc
    28441 aggcatcctg atcgtcctgc ggttgaaaca cggcgctgga ttgcggatag gcgctacaag
    28501 ccggttcatg gcttccattt aaatgaagaa caatcagttt cttttgctgc gtattttgct
    28561 gtaatgcttg cgataaatgc ggcaacaaca attcatcaaa tcctctgaca taaactgttt
    28621 ccatggcgcg catggcgata ctggtaactg cagtaccatt ctgccgaaaa gcggattgcg
    28681 agcttagcca gaaagtctga aatcctgctt gattagccat attaataatg ttgtcggggt
    28741 aattatgaat gtcatgactc aaaacagaat cagcagttaa agaaagggga accgacagcg
    28801 cagtgtaagg tgcgccgctt attgcttgat taaacagttt gatctgtttt ctttgtgctt
    28861 caacttgcgg tgtcgtagag cgtgtatatc catacaaaga catattgtcg acacgtacag
    28921 actcccccac aatcaacacg taggtatcaa tacctgtatc cctgactgat aattgaaaat
    28981 acggcaccgt gtttgcaatt gagagtaatc tttgatgctc tttcgctgct aaagcaaaat
    29041 agttgagatt gaaaaacggc gtataggtag caaatcgcga cgctagtata tatggactga
    29101 acgttttttt attttttgca tctttataag caaattgaca agcggaaaat aaactgcccg
    29161 aaatgacaat cagcaataat attcctgtca ctttttttgt cggcaaggaa acatcatatt
    29221 ttattattac tgcccaaaaa agaagggata aaaaagcaaa ggcacataga taagggatat
    29281 acatccccag cattttgaca acttcatccg gatcactctg gagcacacta atcgcaaatc
    29341 catcattgaa agttgtacca aaggtacacc atgaatagag gctaatactc atgtctgccg
    29401 cagtcagtaa cacaaatggt gcggcaacaa gcagacgaag cggaagggat gatatacgtt
    29461 ttacaaccag gatggtcagg acgaagaaca gcaatcgctg aaccatatgc gcgccggaag
    29521 aggcgactaa gttcgtaata ccaaaaacaa taacacaaag atacaacaac caatgagatt
    29581 taaaacttct agcaaatttg ttgaatactg tcatccagaa atccaagttc acaaccaata
    29641 taaacaatta tcaacaatgt tcaattttat atcagacatg tttttcaact ctgatccata
    29701 tcagaccgaa acactgttag ttttttcttt ttaaatgatg cggccattgc gttctcagta
    29761 attaaataac attatcaatg cgtctctaaa cattacgcag aacgtagcca tcagtagtta
    29821 tactgacagg agcaggatgg tttatcgaga agtaaacata tccgtattcg taccggaact
    29881 ggagttgaca caataaagtg ccaattatgt cagcagaagg gaaaagttta taactaaggc
    29941 gtaccggcac catcgtttca aggtaccagc tacgagtaaa gcaactggac gagatacaga
    30001 tacctgacaa ccattcctca gaccaggacc aaaacgaaaa aagacgcttt tcagcgtctc
    30061 ttttctggag tttggtaccg aggacgggac ttgaacccgt aagccctatt gggcactacc
    30121 acctcaaggt agcgtgtcta ccaattccac cacctcggca cggatactac tttttagttc
    30181 gggatatcgc tggtcggctt agccggagca gctggttgag tttgttcggt tttcgccggt
    30241 gcactcagat tttcccattc gctaccttta ttggttttgt tgctgttgat gttacccagc
    30301 accagactga tgatgaagaa taacgttgcc agcagcgccg tcatgcgggt catgaagtta
    30361 ccagaaccac ttgaaccaaa cagcgtagcg gaagcgcctg ctccgaagga ggctcccata
    30421 tcagcgcctt taccttgctg cagcatgatc agaccaacaa ggccaattgc cacaataagg
    30481 aaaactacca aaagagcttc gtacataatc aacctgttcc ttgcggagtt gccgcgtacc
    30541 aatgcttcaa ccaataaagc gggagttttt aatctttccc actgaagcgg gtgtgaatac
    30601 taaccaaagc gaatgacctt cgcaagggca attttatcgc attgtatcaa ctgcggaaaa
    30661 aaacagcaaa accgatgtat tcgctgagaa aaaaggcgac aatcgccgcc tttttagcca
    30721 gtcatttatc gttttaaacg gcttttactg catcggcgat gcggtgtgca aattcagtca
    30781 cctgcgcttc gtcttcgcct tccaccatca cgcgaattaa cggttcggtg ccggatttac
    30841 gcagcaacac gcgtccacgg ttgcccagcg cagcttcaac ctctgcggtc acggctttaa
    30901 ctgactcatg ctcaagcgga tcgccgctac ctgcggtgta acgcacgtta accagaatct
    30961 gcgggaacat tttcatgccg ctgcaaaggt cgtgcaggct catatggtta cgtgccatcg
    31021 ccgccagcac ctgcaagcca gcaacgatgc cgtcaccggt agtggtttta tccagcagga
    31081 tcacatgacc ggaattctct gcaccgatac gccagccttt ctcctgcatt ttttccagta
    31141 cgtagcggtc acccactttc gcgcgcgcaa atggaatacc taactgtttc agcgccagct
    31201 caagccccat gttactcatc aatgtaccca cagcaccacc acgcagctgg ccctgacgaa
    31261 gaccttcacg cgcgatgata tacatgatct gatcaccatc gactttattg ccttcatggt
    31321 caaccataat cacgcgatcg ccgtcgccgt cgaaggcaat accgagatcc gctttttcag
    31381 ccagcacacg agcctggagc gcgcgaacgt cggtagcccc cacttcggca ttgatgttta
    31441 caccgttcgg ctcacaaccg atagcgataa cgttcgcccc cagttcacgc agcacgttcg
    31501 gcgcgatgtg ataagtcgca ccgtttgcgc aatccaccac aatcttcagt tcgctgaggc
    31561 taagttcgtt cgggaacgtg gctttgcaaa actcgatata gcgacccgca gcatcaacga
    31621 tacggctggc tttacccagt tctgccgaat caacacagct gatctctttt tccatttccg
    31681 cttcgatggc ctcttctacc gcatccggta gtttggtgcc gtcgatagag aaaaatttaa
    31741 tgccattatc gtagaacggg ttatgcgatg cagatatcac aattccggcc tctgcgcgga
    31801 aggtacgcgt cagataagcc acggccggtg ttggcatcgg gccagtgaag agtgcggaaa
    31861 ggcccgctgc cgccagaccc gcttccagtg ctgactccag catatagcca gaaatacgcg
    31921 tgtctttacc aataatgatc ttacgggagc cgtggcgcgc cagcacttta cccgcggccc
    31981 aacccagctt aagcacaaaa tcaggtgtga tcggcgcatc ccctacacga ccacgaatcc
    32041 catcggtacc gaaatattta cgattactca tagcgtttgt tttcctttgc agacagagtg
    32101 gcttccacca cccgcatcgc ttctacggtt tctttgacgt catgaacacg aataatgtgc
    32161 gcgccttgca ttgcggcaat gaccgcacag gccagactac cgctcaggcg ctcggacggc
    32221 cccacgttca gcagctgccc aatcatcgat tttcgtgaca tacctaccaa cagcggcagg
    32281 ttgaaatggt gaaattcagc caggcgcgcc agtaatgaat agttatggga gagattttta
    32341 ccgaaaccga atccggggtc gagcaacaat ttctcttttg cgatacccgc ctgctcgcaa
    32401 cgtgctattt gctcaataaa gtagcgattc acttctgcaa agacatcgtc atacttcgga
    32461 gcttcctgca tggtttttgg atttccctgc atatgcatca gacaaaccgg taaaccggtt
    32521 tctgccgccg cctccagagc gccaggttcg gaaagggagc ggatatcgtt aataatgtga
    32581 gcgccaactt tcgctgactc acggatgact tctggtttgg atgtatctac cgagagccag
    32641 acttcgaagc gttgagcaat tgcctcaacc acaggaataa cacgttgcaa ctcttcttca
    32701 acgctaactt ccgccgcccc tgggcgcgtg gactcgccac caacgtcaat gatcgtcgcg
    32761 ccagcgttga tcatcagatt cgcatgtttc accgcatcta tcagcgagtt atgcgtgcca
    32821 ccatccgaaa aggaatcagg cgtgacgttg aggatcccca ttacgtgagg atggctaagg
    32881 tccagtgaag tgccctgggc aaagagtttc atggtgttat ccctggtatg aattgataag
    32941 aaaaaacccc ggagcacgcc ccggggtttt cggtacaaat acagtcatct gatgcgggaa
    33001 cttacttgtc gcctaactgc tctgacatgg tgttacccgg gttcggcgta cgcggttcat
    33061 caaccggacg aggagccttt ggactaccat tgtcgccaga attgttagaa gcgcctggtt
    33121 cttcccagcc cgctggcgga cgtacatcgc gacgtgccat caggtcatca atctgcggtg
    33181 cgtcgatagt ctcatatttc atgagagcat ctttcatcgc atgcagaata tccatattgt
    33241 cggtcagaag ctgacgcgca cgattatagt tacgctcaat cagtgctttc acttcctggt
    33301 cgatgatacg tgcagtttca tcggacatat gtttcgcttt cgctacgcta cggccgagga
    33361 acacttcacc ttcttcttcc gcgtacagca gtggacccaa tttctcagag aagccccact
    33421 gagtcaccat attacgcgcc aggttggtcg caactttaat atcgttggac gcaccggtag
    33481 aaacatgttc cggcccgtag atgatctctt ctgccagacg accaccgtac agcgtagaaa
    33541 tctggctttc cagtttctga cggctggcgc tgattgcgtc gccctcaggc aagaagaaag
    33601 tcacacccag cgcacgaccg cgtgggataa tcgtcacttt gtgcaccgga tcgtgttccg
    33661 gcaccaagcg accgataatc gcatgacccg cttcgtggta agccgtcgat tctttctgcg
    33721 cttccgtcat caccatggag cgacgttccg cacccatcat gattttatct ttcgcttttt
    33781 cgaactcaat catcgacaca acgcgtttgt tgccacgagc agcgaacagt gccgcttcgt
    33841 ttaccaggtt cgccaggtca gcaccggaga aaccaggagt accacgggca atgattgccg
    33901 cgtcgatatc gggtgccaat ggtacgcgac gcatgtgaac tttcaggatc tgctcacgac
    33961 cgcgaacatc tggcaagcca accacaacct gacggtcgaa acggccagga cgcagcaggg
    34021 ctgggtcaag aacgtccgga cggttagtcg cggcgataac gatgatacct tcgttacctt
    34081 cgaagccatc catctcaacc agcatctggt tcagagtctg ttcacgttca tcgtgaccac
    34141 cgcccagacc agcgccacgc tggcggccta cggcgtcgat ttcatcgata aagatgatgc
    34201 acggtgccgc tttcttcgcc tgttcgaaca tgtcacgaac acgggatgca cccacaccga
    34261 cgaacatttc tacgaagtca gaaccggaga tagtaaagaa cggaactttc gcttcgcctg
    34321 caatcgcttt cgccagcaac gttttaccgg tacccggagg accgaccatc aggacgcctt
    34381 tcgggatctt accgccgagt ttctggaagc ggctcggctc gcgcagatac tctaccagtt
    34441 cagcaacttc ttcttttgct tcgtcgcagc ccgcaacgtc agcaaaggtc gttttgatct
    34501 gatcttccgt cagcatgcgc gctttgctct taccaaacga catggcacct ttgccaccgc
    34561 cgccctgcat ttgacgcatg aagaagatcc agacaccaat cagcaacagc atcgggaacc
    34621 aagagatgaa gatagaagcc agcaggcttg gttcttcagg cggttcaccg acaaccttga
    34681 cgttcttggt caacaggtta tccagtaatt tcggatcctg aaccggaatg taagtggtat
    34741 aacggttact atctttcttg gtaacgttga tttcacgtcc gttgatacgc gcttcacgaa
    34801 cctggtcgtt attgacctct tgtaggaagg tagagtaatc caccttacgg ccattagact
    34861 cgctgggccc aaagctctgg aatactgaca tcagcacaac ggcaatgacc agccagagta
    34921 ttaggttttt cgccatgtca ctcaagggat taacctctta ttacaactgt gttaaaaaca
    34981 gcgtcaggat actctatatc cagcatcttt caaactttcg tctgaaatct cccggttagg
    35041 gtttacgccc ggtcgctaca atatacactt cccgcgaacg tgcacgagaa gagtccggct
    35101 tacgaacttt gaccttcgta aacagggagc gaatttccct tagatactca tcgaaacctt
    35161 cgccctggaa caccttcact acaaaactgc cacctggcgc taatacatca cgacacattt
    35221 ctaacgccag ttccaccaga tacatggcac gggggatatc caccgccggt gttccgctca
    35281 tgtttggtgc catatcggac atgacaacct ggactttgct gtcgccaacg cgctccagca
    35341 gtgctttcat caccagttca tcacgaaaat cgccctgaag aaagtccaca ccaacgatag
    35401 gatccatagg taaaagatcg caagcgatga tgcggccttt gccgccaatt tgggtgacca
    35461 catattgtga ccaaccaccc ggagcagcac caaggtcgac aaccgtcatt cccggtttaa
    35521 agagtttgtc actttgctgt atttcatcaa gtttaaacca ggcacgggaa cgtaacccct
    35581 ttttctgtgc ctgttgaaca tatttatcgc taaagtgttc ctgaagccag cggctggagc
    35641 tggcagaacg cttcttacct gtcatttaac tttcccatgg ggataactca tcgtaaccaa
    35701 ttgcgtaaat ttttacgcgc ctatttggtg atatatggga gatggcggta gaatgacccg
    35761 ttttcaatcc caacgtaagc aaaaaatacg atgaatctga gtactaaaca aaaacagcac
    35821 ctgaaaggtc tggcacatcc gctcaagcca gttgttctgc ttggcagtaa tggtttgacc
    35881 gaaggggtgc tggccgagat tgaacaagcg ttagagcacc atgaactcat caaggtgaaa
    35941 atcgccaccg aagatcgcga aacaaaaacc ttgatcgtgg aagctatcgt gcgcgaaacc
    36001 ggcgcctgta atgtacaggt catcggtaaa acgctggtgc tttatcgccc aactaaagaa
    36061 cgtaaaatct cgctgccacg ctaagattat cctaaagtta cacacatttg ctgtgtaaaa
    36121 cgaggggttt tccgcaggca ggagagcaaa atgccacgct ctgttcgttg ataaaaggcc
    36181 gcatagcggc ctttttcctt tctttacaat acatcaacat cttgatgtat tgggtaattc
    36241 ttacaggtat tccaccttaa ttacttcaaa ttctacttcg ccgcccggcg ttttgatgac
    36301 cacaacatca tcttcttctt tgccgatcag gccacgagca ataggcgagt ttacagaaat
    36361 caggttttgt ttaaagtcag cttcgtcatc gccaacgatg cgataagtct gttcttcgtc
    36421 agaatccaga ttcagcaccg ttacggtagc accaaaaata acgcgcccat tgttgggcat
    36481 tttggtgaca tcaatcacct gcgcgttcga cagcttggct tcgatgtctt taatacggcc
    36541 ttcgcagaaa ccctgctgtt cacgagctgc gtggtattcg gcgttttctt tcaggtcgcc
    36601 atgctcacgc gcttccgcga tagcagcaat gatttcagga cggcgcacag atttcagaaa
    36661 atccagctct tcgcgtaatt tttcagcgcc gcgtaaggtc atcggaatag cttgcatttg
    36721 ttatacctct tgaatattcc tgatggggca agtcttcacc caccccggct gttcggcctg
    36781 cccggcataa ctcctcaccg ggaccagaag caaaaaaata ccgacccggg tacaagtccc
    36841 aggtcagcta caattcacat tttgatagtc attttaccct gaagttcccg aagggtcatc
    36901 gtttacttta tagggcgttg cgccgtagta tgacggctcg attccaggtt gttagcgcga
    36961 gattatgcga ttttccagat ttatcatcgg attgaccagc tgtatagcgt tcagtgttca
    37021 ggccgcgaat gttgatgagt acgttactca actccccgct ggtgccaacc ttgccctgat
    37081 ggtgcaaaaa gtcggcgcgt cggcccccgc tattgattac cacagtcagc agatggcgct
    37141 gcccgccagt acgcagaaag tgattactgc gctggcggcg ttgattcaac tcggccccga
    37201 ttttcgtttc accacgacgc ttgaaaccaa aggcaatgtg gaaaacggcg tacttaaggg
    37261 tgacttagtg gcgcgatttg gtgccgatcc gacgttaaaa cgtcaggata ttcgcaatat
    37321 gatcgcgact ttgaaaaaat ctggcgtcaa ccaaatcgat ggcaatgtgt tgatagatac
    37381 ctccattttc gccagccacg ataaagcccc cggctggcca tggaatgaca tgacacaatg
    37441 ctttagcgct ccgcctgccg ctgccatagt tgaccgcaac tgtttctccg tctcgctcta
    37501 cagtgcccca aaacctggtg atatggcttt tatacgcgtg gcatcttatt accccgttac
    37561 gatgttcagc caggtacgca ccctcccccg aggttctgcc gaagcgcaat actgcgaact
    37621 ggatgtcata ccaggcgacc tgaaccgctt tacgctgacg ggatgcctgc cacaacgttc
    37681 tgagccgctc ccgttggctt ttgccgtgca ggatggagcc agctatgccg gggcaattct
    37741 gaaagatgag ttaaaacagg caggtatcac ctggagcgga acactgctgc gccagactca
    37801 ggttaacgaa cctggaacgg tagttgccag taaacagtcg gccccgttgc acgatctgct
    37861 taagattatg ctgaaaaagt cggacaacat gatcgccgat acggttttcc gcatgatagg
    37921 ccatgcgcgc ttcaatgtgc ctggaacatg gcgggccggg tcggacgccg tgcgtcagat
    37981 cctgcgccag caagccggtg tcgatattgg aaacaccatt attgccgatg gttcagggct
    38041 ttcgcggcat aacctgattg cccccgccac catgatgcag gtgttgcaat acattgccca
    38101 acacgacaat gaacttaact ttatctccat gctgccgctg gcgggctatg atggctcttt
    38161 gcagtaccgt gcaggtctgc atcaggcggg cgtggatgga aaagtctcag cgaaaaccgg
    38221 ttcgttgcag ggggtatata acctggcggg attcattacc acagcgagcg ggcaacgaat
    38281 ggcgtttgta caatatcttt ctggctatgc agtagaaccg gcggatcagc gtaatcgccg
    38341 tattccgtta gtgcgttttg aaagccgttt gtataaagat atttatcaga acaattagtc
    38401 aaaaagaaac cccggcacat ggccggggtt ttgctgatta acgcttgtaa atgaactcaa
    38461 cgccttcttc gtcgtcttcg tcccagtcat catcccagtc ctcatcatcc tcttcagcaa
    38521 tctcttcaag ctgctggcga tgataatcat cccacatgaa ttcgactttc tctggctgtt
    38581 tcgcttcttc agcctgcacg accgggtttt caatgataaa ggtcatcaca tcccagcaga
    38641 gatctttcac gcccagtcca ctcgccgcag agatcagata atatttatct tcccagccca
    38701 gcgcttcagc aatcgctttc gctttctctt cagcttctgc cttatccagc agatcgatct
    38761 tattgaacac taaccaacgc ggtttcgccg ccagatcctg gctgtatttt tccagctcgc
    38821 tgataataat acgcgcgttt tcaaccggat cggtgccgtc aatcggatcg atatcgatga
    38881 ggtgcaacag gacgcggcaa cgttccaggt gcttcaggaa gcggataccc agacctgcgc
    38941 cttccgcagc gccttcaatc agtcctggaa tatcggcaac aacgaagctc ttttcgttgt
    39001 ccattcgtac cacacccaga cttggcacca gagtggtaaa cggataatcc gccactttcg
    39061 gtttagccgc cgataccgcg cgaataaagg tcgatttacc cgcgtttggc atccccaaca
    39121 taccgacgtc agccagcagc atcagctcca gcagcagctc acgcttatca cccggcgtac
    39181 cgttggtttt ctgccgtggt gtacggttaa cggacgattt gaaacgggta ttgcccagac
    39241 cgtgccagcc gcccttagca accagcagac gctgaccgtg tttagtcata tcgcccatgg
    39301 tttcaccagt accctggtcg attacacgcg tacctaccgg cactttaatc gtcacgtctt
    39361 taccgcgctt accggtacaa tcgcggcttg cgccgttctg accgcgctct gcacggaaag
    39421 atttttcaaa acgataatcg ataagcgtgt tcaggttttc gtcggcttcc atccatacgt
    39481 caccaccatc accaccgtcg ccgccatccg ggccgccttt cggaatatac ttttcgcggc
    39541 ggaagctcac gcaaccatta ccgccatcac ctgcaacgac cagaatcgat gcttcatcaa
    39601 caaacttcat tttattctcc gtaaatcatt cgcctgcgcg gggttgcgat accaccgttg
    39661 tatgcttacg taatccgccc caaatacgat gaccaatggc ggaatacatc gcgcccgcaa
    39721 ccacgacaaa cgcaccgaga taacctaaaa ggtttaacat cggtctggcg aagaaatcgg
    39781 gccaggccag tgataaaaga tctgaaaaaa acagcgtaaa cagtggggtg agcgtgatga
    39841 tcgcgctcac ctgcgctgcc tgccagcgag ccatcgcttc cgccagggcg ccatatccta
    39901 ccaatgtatt cagtccgcaa aaaattaaac atgcgagctg ccagtggcta agctgcgcta
    39961 tcactcctgg ctttgccaga gggaagagcg caattgtaca taaagtgtac agcaaaaaca
    40021 ggatctgcgg tgaggccagc cgacgcaata aaaccttttg cgccacgcca taactcaccc
    40081 aaaccgtcgc cgcaccgacc ccaaagatga ctccccaggt gtaatcagtg agctttgtaa
    40141 atatctcgac cagactggtg ttaaaaaaca tcaccaggcc gctcaggagc atcaatgccc
    40201 ctacaacctg agtgctgcgc attttctctt tcaggataaa tacgctggca accatcatgc
    40261 caactggcga gagttgccca atcacctgcg aagcggtcgg actcaggtat tgcaaggatg
    40321 agctgaacag gatgaagttc ccaaacagcc cggcggtcgc cactgccaac aaaatcaacc
    40381 agcgtggctt acgaaacacg cgtaatggcg gcaacctctt cttcaccgca agaatggcac
    40441 ccaggccaat actcgccatc aagaaacggt aaaacacgat tgtcggaggt tccatcacct
    40501 ccagcacctg cttcattgcg attggcaacg ccccccagca aattgctgtg gtgagcgcca
    40561 aaagaatgcc aatgcctgcc tgctgcttca taccgtattc cctgcaaagc gcatttgcgg
    40621 attaacgtcg caccatgctc atttttaccg gttatcgaat gtaaaaagcc ccgcaacgtg
    40681 ttgcggggct ttcatccgtt accgggacgc gaaaaactta ttcagcttcg atgctgataa
    40741 atttacggtt tttcgggcct ttaacttcga atttcacttt accgtctgct ttagcaaaca
    40801 gagtgtggtc acgaccgcaa cctacgttag cgccagcgtg gaatttggta ccacgttgac
    40861 gaacgatgat gctacccgcc agaacggatt cgccaccgaa acgcttaacg cccaggcgtt
    40921 tagcttctga atcgcgaccg ttacgtgtgg agccgccagc ctttttatgt gccatttgaa
    40981 atctctcctc aggtcttagg cgctgatgcc agtaattttc acatcagtga accactgacg
    41041 atggccctgc tgcttacgat agtgtttacg acgacgaaac ttaacgattt taactttctc
    41101 gccacgaccg tgagcaacaa cttcagcttt gattacgccg ccatcaacga aaggaacgcc
    41161 gattttgact tcttcaccgt ttgcgatcat cagcacttca gcgaactcaa cagtttcgcc
    41221 agttgcgatg tccagctttt ccaggcgaac ggtctgacct tcgcttactc ggtgttgttt
    41281 accaccactt tggaaaaccg cgtacatata aaactccgct tccgcgcaca ccttttcaat
    41341 gattcagagt gcgctataaa tattcacaat agggcgcgaa tattacgcaa aacgcacgcc
    41401 tttgacaagt gctacagtca atacacgaag aaaaaaaaca caacttgtac ggtaacgttt
    41461 atctgtgcca ttttttcagt acaatcaccc tatattccta accataaacc ctaagttgcc
    41521 tttgttcaca gtaaggtaat cggggcgaaa agcccggctt ttgcgatgaa tttagaaaaa
    41581 atcaatgagt taaccgcgca agatatggcg ggtgttaatg cggcaatcct tgagcagctt
    41641 aattccgacg tccaactgat caatcagtta ggctattaca tcgtcagcgg cggcggtaaa
    41701 cgtattcgtc cgatgattgc tgtactggct gcacgagctg ttggctatga gggaaatgcg
    41761 catgtcacca ttgctgccct gatcgagttt atccacacgg cgactctgct acacgacgac
    41821 gttgtggatg aatcagatat gcgcaggggt aaagctaccg ccaacgctgc atttggcaat
    41881 gccgccagcg tgctggtagg cgattttatt tatacccgcg cgttccagat gatgaccagc
    41941 ctcggttcac tcaaagtgct ggaagtcatg tcagaagctg taaacgtcat cgcagaaggt
    42001 gaagttctgc aactgatgaa cgttaacgat ccggacatca ctgaagaaaa ctacatgcgc
    42061 gttatctata gcaaaaccgc gcgtctgttt gaggctgccg cgcagtgttc cgggattctg
    42121 gctggctgta cgccggagga ggagaaaggc ctgcaggatt atgggcgcta tctcggcact
    42181 gctttccagt tgatcgacga tttactcgat tacaatgccg atggcgaaca gttaggtaaa
    42241 aacgtcggcg acgatctgaa cgaaggtaaa ccgacgctgc cgctgctgca tgcgatgcat
    42301 catggcacac cagaacaggc acagatgatc cgtaccgcca tcgaacaggg taacggtcgc
    42361 catcttctgg aaccggttct ggaagcaatg aacgcttgtg gatctcttga atggacgcgt
    42421 cagcgcgccg aggaagaagc agacaaagcc atcgcagcgt tacaggtgct cccggacacc
    42481 ccttggcgag aagcactcat cggcctcgcg cacatcgctg ttcaacgcga tcgttaatcc
    42541 cctcccttca tcccgcgcag tgcgcgggat gattccagta aattccataa aaacactaat
    42601 tcagcgctat aacacctgaa aaaaagggcg atgaatcttt atatattctg aatattcaca
    42661 ctctttacag gaacttttta gagcaatagg ccatcaggag tatagtgatg cccgacagaa
    42721 gaagtgttct gaatgaaagc gactaactta aggagtgagg aaagatggaa agtaatttca
    42781 ttgactggca tccccctgac atcattgcag gtttgcgcaa gaaaggaacg tcaatggcgg
    42841 cggaatctcg cagaaatggt ttgagttcct caacgctggc aaacgcatta tcgcgcccat
    42901 ggccgaaagg agagatgatt attgcgaaag ccctgggaac tgacccctgg gttatctggc
    42961 catcacgcta ccatgatccg cagacccatg agtttatcga cagaacgcag ttgatgcgta
    43021 gctacactaa accgaaaaaa tgagtggtct ggcggtagcc ccgcgaacgg ggctgccagc
    43081 tctcagacga ttactcgcct ttcacacgct taatatttgc acctaaagcg cgcagtttgt
    43141 cttcaatgcg ttcgtagcca cgatcgatgt gataaatacg atcaaccacc gtcgtccctt
    43201 ccgcaataca gccagccagc accaggcttg ctgatgcacg cagatcggtt gccataacct
    43261 gtgcaccaga aagtttttca acaccatgac aaataacggt attgctttcg atttcggcat
    43321 gcgcgcccat acggctaagc tccggcacat gcataaagcg gttttcaaag accgtttcgg
    43381 tgatgaatcc ggtcccttct gccaccaggt tcaacagcgt gaactgggcc tgcatatcgg
    43441 tcgggaatgc cggatgcggc gcggtacgta cgttaacagc cttcggacgt ttgccatgca
    43501 tatccaggct aatccagtct tcgccgactt cgatgtccgc tccagcgtca cgcagtttcg
    43561 ccagcacggc gtcgagagta tctggctgcg cgttacggca gataattttg ccgcgagaaa
    43621 tcgccgccgc caccaggaaa gtaccggttt cgatacgatc cggtagcacg cgatagacac
    43681 cgccgcctaa acgttccaca ccttcgatga cgatacgatc ggtgccctga ccgctaattt
    43741 tcgcacccag cgtaatcagg aagttcgcgg tatcgacgat ttccggttca cgcgctgcgt
    43801 tttcaataat cgtggtacct tccgccaggg ttgcagcaca catgatggtc accgttgcgc
    43861 caacgctgac tttatccatc acgatatgtg cgcctttcag acgaccatcg acggaagctt
    43921 taacgtaacc ttcttccagt ttgatggtcg cgcctaattg ttcgaggcca gaaatgtgta
    43981 gatcaaccgg acgcgcaccg atcgtacaac cgccaggcag tgaaacttgc ccctgaccaa
    44041 agcgcgctac cagcggcccc agtgcccaga tagaagcacg catggtttta accagatcgt
    44101 aaggtgcgca gaatacatta acgtcgcggg catcaatatg cacagaacca ttacgttcta
    44161 ctttcgcacc cagctgactt agcagcttca ttgatgtatc gacgtctttc agtttcggga
    44221 cgttctggat ctctaccggt tcttccgcca gtagagcggc aaaaaggata ggcagagcag
    44281 catttttagc gccggaaatt gtgacttcgc cctggagctt cgttggcccc tgaacacgaa
    44341 atttatccat ttagtttgtt ctcagttaac aattcatatc cgctaccggc gaatcgccca
    44401 tagctcaaaa gccgttcagt ttgcgatcgc gcgcccactc cgcaggggta tacgctttga
    44461 tcgacacagc atgaatgcgg ttatccgcaa tatattccat cagcgggcca tagaccgtct
    44521 gctgtttttt aacccgactc atgccgtcaa acaactcacc cacggcaata acctgaaagt
    44581 ggctgccatc gccggaaacg tggacttcct ggagggagag agcgttcatc aacacgctct
    44641 gaatttcatt attttccatg ggatcttcaa tcatcagtta ataaaccagc gaaacatctt
    44701 agagcaaagt tgcgctggca taaataagca aaaagcctcg ctgataaatc agacaaggct
    44761 cgacttgcag gcaggtttgc cggacaggcg gttaacgcca tatccggcct gaaaaaattt
    44821 aacgaggcag aacatcagca ggcaaattat acaatttcgc cagggtatac actttgtcgt
    44881 ttaccccctg aagcgtcaca ttgttgccct gctttttcgc cagatcgata agatggagca
    44941 gcagtgccag tccccccgta tccacgcggg acacacggct aagatcgatg caggtaatcc
    45001 ccttcaccgc ttcctcacgc atttcccaaa gcggtagcaa aacgtcctga tccagctctc
    45061 cggataacgc cagcgtgtca cccgtctgca tccagctcag tgactcgctc attatttttt
    45121 ctcttccaga gtgattttct gttgagaaat cgatttcagt tgcgcagtca ggccgtcgat
    45181 acctttggta cgcagcagcg ttccccactc gttttgtttg gtggtgatca tactgacgcc
    45241 ttcagcaatc atgtcgtaag cctgccagtt gcccgtctgg gagtttttac gccactggaa
    45301 gtccagacgc accggcgggc ggccattcgg atcaataatg gtaacgcgaa tcggcacaat
    45361 ggttttatcg cccagcggct gttctggcgc aatctgatag gtttgaccat gatacatcgc
    45421 cagcgcctga ccgtaagcct gcttcaggta ctcacggaaa gcggcaaagt aggcttcacg
    45481 ttgagcaggg gtcgcactct tgtaatactg gcccagcacc agcgcaccgg cgtatttcac
    45541 ctgtacgtat ggcagcagtt cctgatcaac aatggtacgc agataatccg ggttggcccg
    45601 aatttgcggt tgctcattct tcaggcgatc gaacgttttc tgcgccgcct cgtccatcag
    45661 cttataagga ttggtctggt ctgccgcggt tgccgcactc agcggtgcaa tcaccagcaa
    45721 agcgaccatc attaaacgtt taaacatgcg tcggttctcc tgaaattatt tcgttgtacc
    45781 cacaggttca gtggtttcat tattacctgg cgcagcagct ggcgcatcgc cactattctt
    45841 attgtcatcg cctttactac cgtaaaggaa ctgaccaatg agatcttcca gcaccatcgc
    45901 agacttagtg tcctgaattg tatcgccatc cttcaggata gcagtcccca gttccgggtc
    45961 ttcaaaaccg acgtttaatg ccagatattg ttcccccagt aggccggaag tacgaatgct
    46021 cagcgaactg gtatctggaa tgtggttata acgttgttca atttccagcg ttacgcgtgg
    46081 cagataggtt ttcgggtcca gcgtaatatc cgccacccgg cccacaacaa cgccaccaat
    46141 actgaccgga gagcgcgctt tcaggccgcc aatgttatcg aacgtcgcat aaagcgtgta
    46201 ggtcggttca gtacgtatgg acgtcacgtt cgccgccttc aggcaaacaa acagagccgc
    46261 cagcagtgct gctaataaaa agatacccac ccaaatttca ttttttttcg tttgcatgta
    46321 ctcaattccc aaacatcaat gcggtcagca caaaatccag ccccagaaca gccagagacg
    46381 agtggacaac ggtgcgagtg gttgcccggc taatcccggc agacgtcggg atggcgtcgt
    46441 agccgttaaa caacgaaatc cacgtcaccg tgatggcgaa caccacgctc ttaatcagac
    46501 agttgaccag atccatacgc cagtcgacgg cattttgcat tgccgaccag aagaacccgc
    46561 tatcaatgcc tttccaactg acgccgacca gagatccgcc ccagatcccc acggcgacga
    46621 aaataaccgt caacagtggt aatgaaataa ccccagccca gaaacgggga gaaataaccc
    46681 gacgcagcgg atccaccgcc atcatctcca tactggagag ttgctctgta gcgcgcatca
    46741 ggccgatttc tgcggttagc gccgaaccag cacgcccggc aaacaacaac gcggcaacca
    46801 ccggccccag ttcacgcagt agcgataacg ccaccagcat acccagactg gtttccgcac
    46861 tataagtggt cagaaccaga taaccttgca gccccaacac cattccgatg aacacgccag
    46921 aaaccacaat aatcagcatc gacaggacgc cgacattata gagctggcgc accagcagcg
    46981 gcgcatgttt gcgaaattcc ggtttgccga ccagcgcatt gaataacatt aacccggccc
    47041 gcccgaacgt tctcagggtt ttaatccctt tatgtccgag cgacgccagc gcatttaaca
    47101 gcatgagtgg cttaactccc tggtaaaaga tcagcgtgat aatcgccggc aggatagcgg
    47161 aacggaacag gcccgtcagc tatcccgtcc agaaactgac gtacgcgcgg atcaggattc
    47221 gcctgcaacg cctgggcaca gccatgagcg acaatttttt tgtccgccag gatccaggcg
    47281 tgatccgcaa tacttaacac ttccggcaca tcgtgagaaa ccaccacaca agtcacgccc
    47341 agcgcgctgt tcagctcaga aatcagcttc accagtacgc ccatggtgat gggatcttgc
    47401 ccaacaaaag gttcatcaaa catgatgaga tccggctcca gcgcaatcgc acgcgccagc
    47461 gctgcacgcc gcgccatccc accggaaagt tcagaaggca ttagtttagc cgctccacgc
    47521 agccccacgg cctccagctt catcatcacc gtactatgca acaatggcgc gggaagttgg
    47581 gtatgttcgc gcagtggata ggcgacgttg tcaaatacgt tcatatcagt gaacaacgcc
    47641 ccggactgaa ataacatgct catccgtttg cgcactgtat acaggcgcga acgagacatc
    47701 gccggaatat tctcaccatc gaaaaggatc tcaccatgat ctggtgcgat ttgcccgcca
    47761 atcagacgga gtagcgtcgt tttaccgatg cccgatggcc ccatgatcgc cgtgatcttc
    47821 cctcgcggca cggtcaggga aatattatcg aagatgcagc gattgccacg cgtaaaactg
    47881 acatcgcgca tatcgactaa attcgccaca gactgctcca taattcaccc ttcgtcttgc
    47941 gttgattttc taagcatggc gctcaattta accttgaacc caacatattt acagaatatt
    48001 acccgctgtg gttagcgaaa gctggcattt gttttacttt ttagccgcat aaagtcaaaa
    48061 ttaagaatcc gttacggctt tctgaaaatc ttcagcggac cggcgagtat acctgaagaa
    48121 aggacgttag atgcttttag ctacggcact gttaattgtt ggtttacttt tggtcgttta
    48181 cagtgccgac cgcctggttt ttgccgcgtc tattctttgc cgaacctttg gcataccgcc
    48241 gctgatcatc ggcatgacgg tggtcagtat tggtacatcg ttaccagaaa tcatcgtctc
    48301 gcttgccgcg tctctgcacg aacaacgcga tttagccgtt ggtacagccc tcggctcaaa
    48361 cattatcaat attttgctga tcctcggtct ggctgcgctg gttcgtcctt ttaccgtcca
    48421 ttccgatgtt ctgcgtcgtg aattaccctt aatgttgttg gtcagcgtag tggccggttc
    48481 cgtactctat gacggacaac ttagtcgcag cgatggtatc tttctcctct ttctggctgt
    48541 gctatggctg ctgttcattg ttaaacttgc acgtcaggct gaacgtcagg ggactgacag
    48601 cctgaccaga gagcagcttg cagagctgcc gcgtgacggc ggattgcccg tcgcgttttt
    48661 atggctcggc attgcgctta tcatcatgcc agtggccacg cggatggtgg ttgataacgc
    48721 cacggtgctg gcgaattact ttgccatcag cgagttgacg atgggtctga cggcaattgc
    48781 tatcggaacc agcctgccgg aactggcaac cgcaatagcg ggggttcgca aaggtgaaaa
    48841 cgacattgct gtcggaaata tcattggcgc aaacattttt aatattgtca tcgtgttggg
    48901 tttacccgcg ctgataacac caggagagat tgatccattg gcgtacagtc gtgactacag
    48961 cgtgatgttg ctggtgagca ttatttttgc gttgctgtgc tggcggcgct ccccgcaacc
    49021 gggccgtggt gtaggggtat tattaactgg cggatttatc gtatggctgg cgatgttgta
    49081 ctggttatcg ccaatactcg ttgaataact ggaaacgcat tatgtcgcac gtagagttac
    49141 aaccgggttt tgactttcag caagcaggta aagaagtcct ggcgattgaa cgtgaatgcc
    49201 tggcggagct tgatcaatac atcaatcaga atttcacgct tgcctgtgaa aagatgttct
    49261 ggtgtaaagg gaaagttgtc gtcatgggga tggggaaatc ggggcacatc gggcgcaaaa
    49321 tggccgcaac gtttgccagc accggtacac cttcattttt cgtccatcct ggtgaagccg
    49381 cgcatggtga tttaggcatg gtcaccccac aggatgtggt gattgctatc tctaactcag
    49441 gtgaatccag cgaaatcacg gccttaattc cagtgcttaa gcgtcttcac gtaccgttaa
    49501 tctgcatcac cggtcgcccg gagagcagca tggcgcgcgc cgcagatgtg catctgtgtg
    49561 ttaaagtagc gaaagaagcc tgtccgttag ggctggcacc gaccagcagc accaccgcca
    49621 cgctggttat gggcgatgcc ctcgctgtcg cgctgttaaa agcacgcggc tttactgctg
    49681 aagattttgc gctctcacac ccaggcggcg cactgggtcg taaacttctg ctgcgcgtaa
    49741 acgatattat gcatacgggc gatgagatcc cgcatgttaa gaaaacggcc agtctgcgtg
    49801 acgcattgct ggaagttacc cgcaaaaatc ttggtatgac tgtcatttgc gatgacaata
    49861 tgatgattga aggcatcttt accgacggtg atttacgccg tgtcttcgat atgggagtgg
    49921 atgttcgtca gttaagtatt gccgatgtga tgacgccggg gggaatacgc gtgcgccctg
    49981 gcattctggc cgttgaggca ctgaacttaa tgcagtcccg ccatatcacc tccgtgatgg
    50041 ttgccgatgg cgaccattta ctcggtgtgt tacatatgca tgatttactg cgtgcaggcg
    50101 tagtgtaaag attcaaggat aaacaacaat gagcaaagca ggtgcgtcgc ttgcgacctg
    50161 ttacggccct gtcagcgccg acgttatagc aaaagcagag aacattcgtc tgctgatcct
    50221 cgacgttgat ggcgtactgt cagatggcct gatttatatg ggcaataacg gcgaagagct
    50281 gaaagcgttc aatgttcgtg acggttatgg cattcgttgt gcgctcacct ctgatattga
    50341 agtcgctatc attaccgggc gaaaggctaa actggtagaa gatcgttgtg ccacattggg
    50401 gatcactcac ttgtatcagg ggcagtcaaa caaactgatc gcctttagcg aactgctgga
    50461 aaaactggcg attgccccgg aaaatgtggc ttatgtcggc gatgatctca tcgactggcc
    50521 ggtaatggaa aaagtgggtt taagcgtcgc cgtggccgat gctcatccac tgttgatccc
    50581 gcgcgccgat tatgtgacgc gcattgctgg cggtcgtggc gcagtgcgag aagtttgcga
    50641 cttattactc ctggcgcagg gcaaactgga tgaagccaaa gggcaatcga tatgagtaaa
    50701 gccagacgtt gggttaccat tgtgctatca ctggcggttc tggtgatgat cggcattaat
    50761 atggccgaaa aagacgatac cgcccaggtg gtcgtcaaca acaatgatcc cacctataaa
    50821 agcgagcata cggacacgct cgtctataac ccagaagggg cactaagcta tcgattgatt
    50881 gctcaacacg ttgaatatta ttccgatcag gccgtttcgt ggtttacgca gccggtactt
    50941 accacgtttg ataaggataa aatcccgaca tggtccgtaa aagcagataa agccaagctg
    51001 accaatgacc ggatgctcta tttatatgga cacgttgaag tcaacgcact tgtgccggac
    51061 tctcaacttc gcagaatcac gacggataac gcgcagatca atctggtgac gcaggatgtt
    51121 acctctgaag acctcgtcac gttatacgga acaacattta actccagcgg tctgaaaatg
    51181 cgcggcaact tacgcagcaa gaacgccgag ctgattgaaa aggttagaac atcctatgaa
    51241 attcaaaaca aacaaactca gccttaatct tgtgcttgcc agctcacttc tggccgccag
    51301 cattccggca tttgccgtaa ccggagacac tgatcagccg atccacattg aatcggacca
    51361 gcaatctctt gatatgcaag gcaacgtggt tacctttacc ggtaatgtca tcgtcaccca
    51421 gggcaccatc aaaattaatg ccgacaaagt ggtcgttacc cgtccgggcg gcgaacaagg
    51481 taaagaagtg attgacggct acggtaaacc ggcaacgttc taccagatgc aggacaacgg
    51541 taaacccgtt gaaggtcatg cttcccagat gcactacgaa ctggcaaaag attttgtcgt
    51601 tctgacgggt aatgcttatc tgcagcaggt cgatagcaac attaagggcg ataagatcac
    51661 ttacctggtg aaagagcaga aaatgcaggc tttcagcgac aaaggcaagc gcgtaacaac
    51721 cgttctggtg ccgtcgcagc tgcaggacaa aaacaacaaa ggccagaccc cggcacagaa
    51781 gaagggtaat taattcgtta tggcaacatt aactgcaaag aaccttgcaa aagcctataa
    51841 aggccgtcgc gtggtagaag acgtcagcct gaccgtcaac tccggggaaa ttgtcggtct
    51901 gctggggcca aacggtgccg gtaagaccac cactttctat atggttgttg gcattgtgcc
    51961 gcgcgatgcg ggcaacatca ttattgatga tgacgatatc agtctgctgc ctctgcatgc
    52021 acgcgcgcgc cgcggtatcg gctatctgcc acaggaagcc tccattttcc gtcgcctcag
    52081 cgtttacgat aacctgatgg cggtactgca aattcgtgac gacttgtctg ctgaacaacg
    52141 tgaagaccgc gcgaacgagc tgatggaaga gtttcacatt gagcacctgc gtgacagcat
    52201 ggggcagtca ctctccgggg gtgaacgtcg ccgtgtagaa attgcccgcg cactggctgc
    52261 gaatccgaaa tttattctac tcgacgaacc gtttgccggg gttgacccga tctcggttat
    52321 cgacattaaa cgcatcattg aacacctgcg cgacagcggc ctgggcgtac tgatcactga
    52381 ccacaacgtg cgtgaaacac tggcggtttg tgaacgcgct tatatcgtca gtcaggggca
    52441 tttgattgcc cacggcacgc ctacagaaat cttacaagac gaacacgtta agcgtgtata
    52501 ccttggggaa gacttcagac tctgataggg tagaagtttg cgacgtttta gcaggagagt
    52561 acgattctga acatgaagca aggtttgcaa ctcaggctta gccaacaact ggcgatgacg
    52621 ccacagcttc aacaggcgat tcgtctgttg cagttgtcga cgctggaact tcagcaggag
    52681 ctacagcagg cgctggagag taatccgctg cttgagcaaa tcgacactca tgaagaaatc
    52741 gacaccagcg aaacgcaaga cagtgaaacg ctggacaccg ccgacgcgct cgaacaaaaa
    52801 gagatgccgg aagagctgcc gctcgatgcc agttgggaca ccatttacac cgctggtaca
    52861 ccatccggca ccagcggtga ctacattgac gatgagctgc cggtctatca gggcgaaacg
    52921 acgcagacct tgcaggatta cctgatgtgg caggtcgagc tgacaccgtt ttccgacact
    52981 gaccgcgcta ttgctacctc tatcgtcgat gccgttgatg acaccggtta tctgactgtc
    53041 ccgctggaag atattctcga aagtatgggc gatgaagaga tcgacatcga cgaggttgaa
    53101 gccgtcctta agcggatcca acggtttgat ccggtcggtg tagcggcaaa agatctgcgt
    53161 gactgcctgc taatccaact ctcccaattc gataaaacca cgccgtggct ggaagaggcc
    53221 agactgatca ttagcgatca tctcgatctg ttagccaatc acgacttccg cactttaatg
    53281 cgcgtcacgc gtctgaaaga agatgtgctg aaagaagccg tcaatctgat ccagtcgctc
    53341 gatccgcgcc ccgggcagtc gatccagact ggcgaacctg agtatgtcat tccagatgtg
    53401 ctggtgcgta agcataacgg tcactggacg gtagaactca acagtgacag cattccgcgt
    53461 ctgcaaatca accagcacta cgcctcgatg tgcaataacg cgcgcaacga tggtgacagc
    53521 cagtttatcc gcagcaatct gcaggatgcc aaatggttga tcaagagtct ggaaagccgt
    53581 aacgatacgc tactgcgcgt gagtcgctgt atcgttgaac agcagcaagc cttctttgag
    53641 caaggtgaag aatatatgaa accgatggta ctggccgata tcgcccaggc tgtcgaaatg
    53701 catgaatcga cgatatctcg cgtgaccacg caaaaatacc tgcatagtcc acgaggcatt
    53761 tttgaactga agtatttctt ttccagtcac gtcaataccg agggcggcgg cgaagcttcc
    53821 tccacggcga ttcgtgcgct ggtgaagaaa ttaatcgcgg cggaaaaccc agcgaaaccg
    53881 ttgagcgaca gcaagttaac ctctttgctg tcggaacaag gtatcatggt ggcacgccgc
    53941 actgttgcga agtaccgaga gtctttatcc attccgccgt caaaccagcg taaacagctc
    54001 gtttgaccca accgataagg aagacactat gcagctcaac attaccggaa ataacgtcga
    54061 gatcaccgag gcactgcgcg aatttgttag agccaaattt gccaaacttg agcaatattt
    54121 tgaccgaatc aaccaggtct atgttgttct gaaagtggag aaagtcaccc acacctcaga
    54181 tgcaacactg catgtaaacg gtggtgaaat tcatgccagc gcggaaggtc aggacatgta
    54241 cgctgccatt gatggcttaa ttgataagct ggcacgccag ttgaccaaac ataaagataa
    54301 actgaaacaa cactaattgt ccgggcaatt agcatgtgca tggcggcctg ttgtgcggca
    54361 caacgggcca tttgtacggt taatgctccg agcctgttcc actgtttgag tgggcaggtt
    54421 cttaggtgaa attatgacaa ataatgatac aactctacag cttagcagtg ttcttaacag
    54481 ggaatgtacg cgaagccgcg ttcactgtca gagcaaaaaa cgcgccctgg aaatcatcag
    54541 cgagctggcg gcgaaacaac ttagcctgcc gcctcaggtg gtttttgaag ctatcctgac
    54601 gcgggaaaaa atgggcagta ccggtatcgg caatggtatt gccattccgc atggcaaact
    54661 ggaagaagat actctgcgcg ccgttggcgt tttcgttcag ctcgaaacgc ctatagcttt
    54721 cgatgccatc gacaaccaac cggtggactt actttttgcc ctgctggtgc cggcagacca
    54781 aactaaaacg cacctgcata ctctgtcgct ggtggcgaaa cgtctggcgg acaaaaccat
    54841 ctgccgccgt ttacgcgcag cccagagcga tgaagagctg tatcaaatca ttacggatac
    54901 cgaaggtact ccggatgaag cgtagttatt cggtaatgtc tcttttagac gttgtgagga
    54961 gaaacagtac atggtactga tgatcgtcag cggacgttca ggttcaggta aatctgtcgc
    55021 cctgcgtgcg ctggaagata tgggttttta ctgcgtggat aaccttcccg tagtgttgtt
    55081 acccgatctg gctcgaaccc tggccgatcg cgagatttct gccgccgtca gcattgatgt
    55141 tcgtaatatg ccggagtcac cagaaatatt cgaacaggcg atgagtaacc tgcctgacgc
    55201 tttctcaccg caactcctgt tcctggatgc cgaccgtaat accttaattc gtcgttacag
    55261 tgacacgcgc cgactgcatc cgctttccag caaaaacctg tcgctggaaa gtgctatcga
    55321 caaagaaagc gatttgctgg agcctctgcg ttcgcgagcg gatctgattg tcgatacctc
    55381 agaaatgtcc gttcacgagc tggctgaaat gctgcgtacc cgtctgctgg gtaaacgtga
    55441 acgcgaactg accatggtct ttgagtcttt cggcttcaaa cacggtatcc ctatcgatgc
    55501 agattacgtc tttgacgtgc gcttcttgcc gaacccgcac tgggatccga aactgcgtcc
    55561 aatgacaggt cttgataaac ctgtcgccgc gttcctcgac cgccacacag aagtacacaa
    55621 ttttatctac cagacgcgaa gctatcttga gctatggtta cctatgctgg aaaccaacaa
    55681 ccgtagctac ttgacggtcg ccattggttg taccggcggg aagcaccgtt cggtgtatat
    55741 tgcagagcaa ctggcagact acttccgctc gcgcggtaaa aacgtccagt cacgccatcg
    55801 tacgctggaa aaacgtaaac catgaccgtc aagcaaactg ttgaaatcac aaacaagctg
    55861 ggcatgcatg cccggcctgc aatgaagctg tttgaattaa tgcagggttt tgacgctgaa
    55921 gtgctgttac gtaatgacga aggcaccgag gccgaagcca acagcgttat tgcgctgctg
    55981 atgttggatt ctgccaaagg acggcagatt gaagttgaag cgaccggccc acaggaagag
    56041 gaagcactgg ccgccgttat cgccctcttt aactctggtt ttgatgaaga ttaatcttca
    56101 tcactttgac atacaactcc cttcaaactc cccccgacaa taagaaaatc acgtactgaa
    56161 atcgttctca atcaacgtca tttgtacatt ttgtgcgctt ttcactttca gaagaacctt
    56221 aagaaaacct taagaggcat tgtttaggtt ttgtttaagt taatcgacca tactggagat
    56281 cgtcagaaaa tatttccagg agatggcatg attcgcttat ctgaacaaag tcccctgggc
    56341 accggaagac atcggaaatg ttatgcgcat ccggaagatg cccaacgctg tatcaagatt
    56401 gtctaccatc gtggcgatgg tggcgataaa gagatccgcc gtgagttaaa gtactacgcg
    56461 tatcttggtc gccggttaaa agactggagt ggaataccgc gctatcacgg taccgttgag
    56521 acggattgcg gaaccggtta tgtctacgat gtgattgcag attttgacgg caaaccctcc
    56581 attacgctga ccgaatttgc tgaacaatgc cgttacgaag aagacattgc acaactgcgc
    56641 cagctactga aacagctgaa gcgttatttg caggataacc gtatcgtgac gatgtcgctg
    56701 aagccgcaga atatcctctg tcaccacatc agtgagtcag aggttatccc ggtcgtctgc
    56761 gacaacattg gtgaaagcac attgattcca ctggcgacct ggtcgaagtg gtgttgtctg
    56821 cgtaagcaag agcggttatg gaaacgattt atcgcccaac cggcgctggc tattgccctg
    56881 cagaaagatc tgcaacctcg tgagagcaaa acgctggccc tcacttcgcg cgaagcttaa
    56941 tccagctggt gttgttgcat aaacggctca ccgcctaact gatacatctg ccgtaaaatc
    57001 cacgcctgac ggctacgcac gtagcccgat ggcgaggaga ctttgaaacg aagtggatta
    57061 ggtaatacag ctgccagtaa tgcagcttcc gaccgggtaa gtttgctcgc gggtttgtgg
    57121 aaataacgtt gtgccgcagc ttcgacgcca aacacaccgt cgccaaattc ggcgatattc
    57181 aggtaaaccg tcaggatacg ctttttgctc cagactgttt ctatccccag cgttaatcca
    57241 gcttccagcc cttttcgtac ccagctacgc ccatcccata aaaagagatt tttggccgtc
    57301 tgctgagaaa tcgttgaagc accgcgaata cggttttcat tgcgctcgtt gtgcgccagg
    57361 gctttctcaa tggaagcgac atcaaagccc cggtgctcag gaaatttctg atcttctgcg
    57421 gcaatcaccg ccagtcccat ccacggcgag atttgatcca tactgaccca gtcagaatgt
    57481 gccacgtaac gaaaattgcc atgcagccag gcgctgacct gtcgctcgac cattaccgct
    57541 gagaagggaa caggcgcaac actaaacaac gcgatgcccc cgccccagaa aacggcgagg
    57601 acaaccatta accgcaaaag aaaacggcga acgaaactaa acaccgttaa gcggctttta
    57661 ctcattcagc cagaaccagc acgcgggaaa ccagcttatc aatgccgctc gccgcttctg
    57721 caatgttctg cgccagcata tatgctgggg tggtgacaat cttattgtct tcatcaacca
    57781 cgatatcatc gacaggacag ggcacatgct ccgcgcccat ctcttccagc acttctgcgg
    57841 tatcgatatc agtaccgatg gtcaaacgca gcgggaaatc gaaaattttc ggcagcatcg
    57901 ccggggcaat acacataaaa ccaagcggtt ttccggcctg atgcatcgct tgtgccagcg
    57961 cctttaattc acggtcaacg gtgcattcgc taccaagagt ggcaaaattg cttaaattct
    58021 tcgccgcgcc aaaccccccc ggcacaatca acgcatccag ttcagcggca tcggcctggg
    58081 ccagaggacg gatttcaccg cgcgttattc gtgccgcttc aatcagcacg ttgcgcgttt
    58141 ccgtcatcgc ttcgccagtt aaatggttga taacatcaac ctgctgctta tccggtgcaa
    58201 agcagaccgc ctgcgcaccg ctgcgtgaaa tagctaacaa cgtcaacacc gcttcatgaa
    58261 tttcagaacc gtcatagacg ccgcatccgc tcagaattac gccaattttc ttcattgtga
    58321 tcatcctttc gcaattgact gaaacacatt aattttttta ataaaaatgg tacgcatcac
    58381 acatttaact gattcatgta acaaatcatt taagttttgc tatcttaact gcgtgcggcc
    58441 tgaaaaacag tgctgtgccc ttgtaactca tcataataat ttacggcgca gccaagattt
    58501 ccctggtgtt ggcgcagtat tcgcgcaccc cggtctagcc ggggtcattt tttagtggct
    58561 tttgccaccc acgctttcag cacttctacg tcgtgacgcc actcttcttt catctcttca
    58621 atccattcac cgacgttatc ttcccaggca ggaaggtcag gagactgaat ttgctgaccc
    58681 agctgttgca gatggcgtaa ccccactgac cccgccgcac ctttaatttt atgtccttcc
    58741 tcaacaatgc cttttttatc ctgcgccgtc agattcgact ccagcacgct gacatagccc
    58801 ggcatcatct tctcaaacac cgctaacccg tcggtgatca gcttcggtcc tacaagttcg
    58861 agatactgtt ccagcatggg aatatcgagc aatgcttctg atttactgtt ctcttctgtc
    58921 gtcaccgtac tctcctcatc atcctgggta tcccagaatt tcttgatcat cgcggttagc
    58981 gccggaaccg aaagcggctt actcagcaca tcatccattc cagcattgag gtactcttgt
    59041 ttgtctttca gcacgttagc ggttaaggcc accagcggcg gtaaatcctc gcgcggataa
    59101 cgcttcgtca gttcacgaga gatatccagc ccggtcatat ctggcaactg aatatccagc
    59161 aacaccaggt cgtattcgcc cggtttaaac atctccagcg ccgccttgcc ggtcatggcg
    59221 acatcaacgc tgttacctaa tttttccagc acagaacgcg caacaatcac gttcagttca
    59281 atgtcttcca ccagcagcac attcagcgcc ggtaaaggca tatcgtcttc atcaaacgca
    59341 tcatcgacct cttctgctac cgacggtgcg tggatcgtca acgtaaaggt tgaacctttg
    59401 ccctgttcgc tggtaaccgt aatatcgccg cccatatttt tcgccagacg acgagaaacg
    59461 gccagaccaa taccggtgcc ggtggcaggt ttaccgccat gactgtcttt cacctggtaa
    59521 tacatggcga aaattttatc cagctcatcc tgcggaatgc cgataccaga gtcttccact
    59581 tcaaaatgca gcatatcgcc ttcatcgtag cgcacgcgca cggtaacctg gccttgctgg
    59641 gtgaatttga cggcgttact gatgaggttc cacaggatct gccgtaaacg cgtcccgtcg
    59701 gtaatgacct gatgcggtaa tggcagcgtc ggctccaggt taaagcgcaa tcctttttgt
    59761 tgcgcctgca atgcggagag attttccaga tcggcaagga agctggtgaa atcaaccggt
    59821 tgattatcaa gctggacctt gcgccgttcc atcttatcca tgtcgataat atcgttaaag
    59881 atattcccca gcgtgacggc cgaaacatgg atggtcttga gatatttttc ctgctcggcg
    59941 gtgagttcgg tatccagcag aatgcggctc agaccgacga taccgttcag cggtgtacgc
    60001 aattcgtgac tgatggtgga gataaacgtc gttttgtcgc ggctggcccg ttcaagcgca
    60061 tcctgatacc gcttacgctc ggtaatgtcg cgaccaaagc ccatcaaacc gtgacgttta
    60121 cccacgcggt cgtagtacgg cactttacgg atttcaaagc aggctttgcg cccgtccggg
    60181 taatccagcc actgttcata ggtcagtgac acattatgac ggaacacttt ttcatcggtt
    60241 tcaatgactt ttgcggcggc ttccggtgag taaacatcag caggtttcag gtgaaccagt
    60301 tgtttttcgc tttttccggt cagcagctcc atcgcgcggt tacagccgga aaactcttta
    60361 tcttcgttac gataaaaaac caggtcgggt gaagcatcaa ggaaggaacg taagaatgag
    60421 gattgctgct cgagctgaat ttgtgtctct tcgcgctctt tgatttcaat tttcagttgg
    60481 ccgaaggttt cctgcagttc tgcttccgct ttttcacgaa cggcaatttc ctgatttagc
    60541 tgggcaatat tatcttttaa ctgaacgttg aggctcaaat cgcgctcgcg catctcctcc
    60601 agtttttgca ccagccgtga cagacgttgt cgtgactcct ccagttgctc gacgaccacc
    60661 gatagaaagt agaccgccca cggcgtaatc agcaaaccaa agaagataga acgaataaca
    60721 tcaatgcttt cgacctgacc atgcagcacc atggttaccg ccatttgtac cacaatggca
    60781 agaacgacga gggccagcgc cagcaacatt gagaagcgca ccagacctaa cttcatcatc
    60841 aggtcaacat aatactgcgc cagcagacga atttgcttca ttagggaatt ccttcacgac
    60901 aacctgcccc aataataccc aattctgaca gttacgttat aaattgtgtg agaaatgcgg
    60961 aattcccctc aacgacctac tccgttggag gtagccacgg acgtcccagc gccgatccct
    61021 gtacgccatg ttcattcaga tagcgatcca gctcgaccat tcccgtccat cgattttcgc
    61081 accacagcgg agcaagcagc gtcgggcgac gggcgctggc ggaaatgcgg tggtagatca
    61141 cttccggtgg cgtatggcga atcatttctc cggcagtgag cgtgtaatcc tccagttcaa
    61201 taccgttcaa acgccccgct tcccaggctt tcgccataat gctgcctttc acaatatgca
    61261 gcggatgcag ctttatgccg tccacgccgg tttcaaccac tcgctccagc gtttgcaaac
    61321 attcagcctg cccttcacca ggcaggccga caattaagtg ggaacacacc ttcagcccgc
    61381 gctgacgtgc cagctgggtt gtacgctgat aacaggcaaa atcatggccg cggttgatgc
    61441 gatgcagtgt tttgtcgtgg gcggtttgta gccccagctc cagccacact tcgtaaccct
    61501 ggtccttata ttcgcaaagc agatccagca ccgcatccgg cacgcagtcc gggcgggtac
    61561 caacacacaa accgacaata ttggcctggc tcaccgcctg ctgatacata gaacgcagca
    61621 cctgaacttc cgcaaaggtg ctagtatacg cctgaaagta ggccagatag cgtttagcgc
    61681 ggttaactaa attcgcctgg tgcgccagtt gctcggcaat ggaacgatgc tgctgcgctt
    61741 catcggcaaa cgaggcaaca ttacagaatg tgcagccacc acgcccgatg gtaccgtcac
    61801 ggttagggca gctaaaaccg ccgtgcagcg tcagcttatg caccttttgc ccataacgac
    61861 gggtgagatc accaccaaac atattgacta atttctgtaa ctgcataatc tgatagaccg
    61921 cgccttgaaa agaggccaaa gcctgccatt tttagctcaa ttcggcgatg acctggatca
    61981 atcgtcccgg cctgctttta tcaactgcat aatcaatcaa aattaccgaa atttcatgca
    62041 taatcacata aatcactttt gcttatcttg tgtcagattt ttttatctcc ttatggattt
    62101 taggcaaaaa cagtagcatg aaacgtcatt accaattaag gcagtataaa atgctggttt
    62161 tctcgtcagt tcaaggtagg ataagggtta acacgccttt atgacagtca ggaattgact
    62221 gtttctctaa cgacttccct tttagcctta aagataaaat tcattttaat ttcagttgct
    62281 taataaagaa ttttgcgcta aagcacattt ctgtaccaat aagcttgcca tttgacctgt
    62341 atcagctttc ccgataagtt ggaaatccgc tggaagcttt ctggatgagc agcctgctca
    62401 tcatatttat gcagtaattg agatcccctc ttcaccgtat taaccgatgc gaaaaggaca
    62461 acaagggggc gaatacgagg cgcgcgtatg acacgcaacc cccgtcgcca cgctctttct
    62521 gtgcccgtgc gcagcggttc ggaagtgggg ttcccgcaga gcctggggga ggttcacgat
    62581 atgttgtacg ataaatccct tgagagggat aactgtggtt tcggcctgat cgcccacata
    62641 gaaggcgaac ctagccacaa ggtagtgcgt actgcaatac acgcactggc ccgcatgcag
    62701 caccgtggcg cgattctcgc cgatggtaaa accggtgacg gttgcggctt gctgttacaa
    62761 aaaccggatc gcttttttcg catcgttgcg caggagcgcg gctggcgttt agcaaaaaac
    62821 tacgctgtcg ggatgctctt cctgaataaa gatcctgaac tcgcggctgc cgcacgccgc
    62881 atcgttgaag aagagctgca acgcgaaacc ttgtcgattg tgggctggcg tgatgtcccc
    62941 actaacgaag gcgtgctggg tgaaatcgcc ctctcctctc tgccccgcat tgagcaaatt
    63001 ttcgttaacg ctccggcagg ctggcgtccg cgggatatgg agcgccgtct gtttatcgcc
    63061 cgccgccgca ttgaaaagcg tctcgaagcc gacaaagact tctacgtctg tagcctgtcg
    63121 aatctggtga acatctataa aggtctgtgt atgccggcgg atctgccgcg cttttatctg
    63181 gatcttgcgg acctgcgtct ggaatcggcc atctgcctgt tccaccagcg cttctccact
    63241 aacaccgtac cgcgctggcc gctggcgcag ccgttccgct acctggcgca taacggtgaa
    63301 atcaacacca tcaccggtaa ccgccaatgg gcgcgtgcgc gtacctataa attccagacg
    63361 ccgcttatcc ctgatctgca cgacgccgca ccgttcgtca acgaaaccgg ctctgactcc
    63421 agttcgatgg ataacatgct ggaactgctg ctggcaggcg ggatggatat catccgcgcc
    63481 atgcgtctgt tagtgccacc cgcctggcag aacaacccgg atatggaccc ggaactgcgt
    63541 gccttctttg actttaactc catgcatatg gagccgtggg atggcccggc gggcatcgtg
    63601 atgtccgacg gtcgttttgc cgcctgtaac ctcgaccgta acggtctgcg tccggcgcgc
    63661 tacgtcatca ccaaagataa gctcatcacc tgcgcctctg aagtcggtat ctgggattac
    63721 cagcctgacg aagtggtcga aaaaggccgc gtcgggccag gcgaactgat ggttatcgac
    63781 acccgcagtg ggcgtattct gcactcggca gaaaccgatg acgatctgaa aagccgccat
    63841 ccatataaag agtggatgga gaaaaacgtc cgcagactgg taccgtttga agatctgccc
    63901 gatgaagaag tgggtagccg cgaattggac gacgacacgc ttgccagcta ccagaaacag
    63961 tttaactaca gtgcggaaga gctggactcc gtaattcgcg tactgggcga aaacggtcag
    64021 gaagcggtcg gttcgatggg cgatgatacc ccattcgccg tgctctccag tcagccgcgc
    64081 attatttacg actacttccg ccagcagttt gcccaggtga ctaacccgcc aatcgacccg
    64141 ctgcgtgaag cgcatgttat gtcgctcgcc accagtatcg gtcgtgaaat gaacgtcttt
    64201 tgcgaagcag agggccaggc gcaccgttta agctttaaat cgccgattct gctctactcc
    64261 gatttcaaac agctcacgac gatgaaagag gagcactacc gcgcagatac gctggatatc
    64321 acctttgacg tcactaaaac cacgctcgaa gcgacagtca aagagctgtg cgacaaagcc
    64381 gaaaaaatgg tacgtagcgg caccgtgctg ctggtgctct ccgaccggaa tatcgctaaa
    64441 gatcgcctgc cggttccagc cccgatggcg gttggcgcga tccagacccg tctggtcgat
    64501 caaagcctgc gttgcgatgc caacatcatc gtcgaaaccg ccagcgcccg cgatccgcac
    64561 cacttcgccg tgttgctggg cttcggcgcg acggctattt atccatacct tgcctatgaa
    64621 acgctgggcc gcctggtaga cacccatgcg attgccaaag attatcgtac cgtgatgctc
    64681 aactaccgta acggcatcaa caaaggcttg tacaaaatca tgtccaaaat gggcatctcc
    64741 accatcgcct cttaccgctg ctcgaaactg tttgaagcgg tcggtctgca cgatgatgta
    64801 gtgggcctgt gcttccaggg ggcggtcagc cgcattggtg gggcgagctt tgaagacttc
    64861 cagcaggatc tgctgaacct gtcgaaacgt gcctggctgg cgcgtaagcc catcagccag
    64921 ggcggcctgc tgaaatacgt ccacggcggc gaataccacg cttacaaccc ggacgtggtg
    64981 cgcacgctgc aacaggcggt acaaagcggc gagtacagcg actatcagga atacgcgaag
    65041 ctggttaatg agcgtccggc aaccacgctg cgcgatctgc tggcaattac gccgggtgaa
    65101 aacgcggtca acattgctga tgttgaaccg gcaagcgaac tgtttaaacg ctttgatacc
    65161 gccgcgatgt ctatcggcgc gttaagcccg gaagcccacg aggcgctggc ggaagcgatg
    65221 aatagcatcg gcggtaactc gaactccggt gaaggcggcg aagacccggc gcgctatggc
    65281 actaacaaag tgtcgcgcat caagcaggtg gcttctggtc gcttcggcgt tactccggcg
    65341 tatctggtca atgccgacgt cattcagatt aaagtcgccc agggcgcgaa gccgggcgaa
    65401 ggcggtcagt tgccgggcga caaagtcact ccttacatcg ccaaactgcg ctattcggtg
    65461 cccggagtga cgctgatctc cccgccgccg caccacgata tctactctat cgaggactta
    65521 gcgcagctca ttttcgacct caagcaggtt aacccgaaag cgatgatctc cgtgaagctg
    65581 gtttccgaac cgggcgtagg caccatcgcg actggcgtgg caaaagctta tgccgattta
    65641 atcaccatcg caggctatga cggtggcacc ggcgcaagcc cgctgtcgtc agtgaaatac
    65701 gcaggttgcc cgtgggagct ggggcttgtt gaaacccagc aggcgctggt tgccaatggt
    65761 ctgcgtcata agatccgttt gcaggtcgat ggcggcctga aaaccggtgt tgatatcatc
    65821 aaagcggcga ttctcggcgc agaaagcttc ggcttcggca ctggcccaat ggtcgcgctc
    65881 ggctgtaaat atctgcgtat ttgccacctg aacaactgcg caacgggtgt ggcaactcag
    65941 gatgacaaac tgcgtaagaa tcactatcac ggcctgccgt tcaaggtgac gaattacttt
    66001 gagtttatcg cccgtgaaac ccgcgagctg atggcacagc tgggggtaac gcggctggtg
    66061 gatctgattg gtcgcaccga cctgctgaaa gagctggacg gtttcaccgc caaacagcag
    66121 aaactggcgc tgtcgaagct gctggagacc gccgaaccgc atccaggtaa ggcactctac
    66181 tgcaccgaaa acaacccgcc gtttgataac ggcctgctga acgcgcagtt gctgcaacag
    66241 gcgaaaccgt ttgtcgatga acgccagagc aaaaccttct ggttcgatat ccgcaatacc
    66301 gatcgctccg tgggcgcgtc gctttcaggc tatatcgccc agacgcacgg cgatcagggg
    66361 ctggcagccg atcctatcaa agcgtacttc aacggcaccg caggccagag tttcggtgta
    66421 tggaacgcgg gcggcgtgga actgtacctg accggggatg ccaacgacta tgtcggtaaa
    66481 ggcatggcgg gcggcttaat cgccattcgt cctccggttg gttccgcctt ccgcagccat
    66541 gaagcaagta ttatcggcaa cacctgcctg tatggcgcga ccggtggtcg tctgtatgcc
    66601 gcaggccgcg cgggtgaacg tttcggcgtg cggaactccg gtgctatcac cgtggtagaa
    66661 ggcattggcg acaacggctg tgaatatatg acgggcggta ttgtctgtat tctgggtaaa
    66721 accggcgtta acttcggtgc gggcatgacc ggcggcttcg cttacgttct cgatgaaagc
    66781 ggtgatttcc gcaaacgcgt taacccggaa ctggtcgagg tcttaaacgt tgacgatctg
    66841 gcgatccatg aagagcatct gcgcggtctt atcaccgagc atgttcagca tactggctct
    66901 cagcgcggtg aagagattct ggcgaactgg tcaaccttcg ccactaaatt tgcgctggtt
    66961 aaaccgaagt ccagtgatgt aaaagcactg ctgggtcacc gtagtcgtag cgcagcagag
    67021 ttgcgcgtgc aggcgcagta aggggtagca acaatgagtc agaatgttta tcaatttatc
    67081 gacctgcagc gcgttgatcc gcctaagaaa ccgctgaaga tccgcaaaat tgagtttgtt
    67141 gaaatttacg agcagttttc cgaaggccag gccaaagcgc aggctgaccg ctgcctgtcg
    67201 tgcggcaacc catactgcga gtggaaatgt ccggtacaca actacatccc gaactggctg
    67261 aagctcgcca acgaggggcg tatttttgaa gcggcggaac tgtcgcacca gaccaacacc
    67321 ctgccggaag tttgcggacg agtctgcccg caagaccgtc tgtgcgaagg ttcctgcact
    67381 ctgaacgatg agtttggcgc ggtgaccatc ggcaacattg agcgttatat caatgataaa
    67441 gcgttcgaaa tgggctggcg tccggatatg tccggcgtga agcaaaccgg taaaaaagtg
    67501 gcgattatcg gcgcaggccc ggcaggtctg gcgtgtgcgg atgtcctgac gcgtaacggc
    67561 gtaaaagccg ttgtcttcga ccgtcatcca gaaattggcg ggctgctgac cttcggtatt
    67621 ccggccttca agctggaaaa agaggtaatg acgcgtcgcc gtgaaatctt caccggcatg
    67681 ggtattgaat tcaaactcaa taccgaagtg ggccgcgacg tgcagctgga cgatctgctg
    67741 agtgattacg atgccgtctt ccttggcgtc gggacttatc agtcaatgcg cggcgggctg
    67801 gaaaacaaag acgccgatgg cgtgtacgca gcgctgccgt tccttatcgc caacaccaaa
    67861 cagttaatgg gctttggtga aacccgcgac gaaccgttcg tcagcatgga aggcaaacgc
    67921 gtggtggtcc ttggcggtgg cgacactgcg atggactgcg tgcgtacgtc cgtgcgccag
    67981 ggagcgaagc acgttacctg tgcctatcgt cgtgatgaag agaacatgcc gggttcccgc
    68041 cgcgaagtga aaaacgcgcg ggaagaaggc gtagagttca aattcaacgt ccagccactg
    68101 gggattgaag tgaacggtaa cggcaaagtc agcggcgtaa aaatggtacg taccgaaatg
    68161 ggcgaaccgg atgccaaagg ccgtcgccgc gcggagatcg ttgcaggttc cgaacatatc
    68221 gttccggcag atgcagtgat catggcgttt ggtttccgtc cacacaacat ggaatggctg
    68281 gcaaaacaca gcgtcgagct ggattcacaa ggccgcatca tcgccccgga aggcagcgac
    68341 aacgccttcc agaccagcaa cccgaaaatc tttgctggcg gcgatatcgt ccgtggttcc
    68401 gatctggtgg taaccgctat tgccgaaggt cgtaaggcgg cagacggtat tatgaactgg
    68461 ctggaagttt aagctctgtc ccagcgccgc ctcctcagaa aagagtcggc gctgaaattc
    68521 tcctttcttt ctggaaagcg cctcgcataa tctgaagaaa accaatgtaa tacgctcatc
    68581 tggattgtta ccggtagatg cccggcgtat agctgattcc attattcaac gcagcgccat
    68641 ataaagggac agataatgga atcgctctca gagagaacct caacaggcta ccagcaaatc
    68701 cacgacggta ttattcacct ggtcgatagc gcccggacgg aaacggtacg tagcgttaac
    68761 gcgctgatga ccgcgacata ctgggaaatt ggccgacgaa ttgtcgaatt tgaacaaggt
    68821 ggcgaggcaa gggctgcgta tggtgcgcag ctaatcaagc gactatcaaa ggatttaagt
    68881 ctaaggtata agcgtgggtt ctctgcaaaa aacttacgcc aaatgaggct tttttacctc
    68941 ttttttcaac atgttgaaat tcgccagaca gtgtctggcg aattaacacc attgggaatt
    69001 ccgcagacac catctgcaga attcccatcg gcaaagattt ggcagacgct gtctgccaaa
    69061 ttgagaggct gtttgatatt tccacagctt ttgtattgca tctcctattt tcggtttgta
    69121 tattcatgca aataaacgaa ggaaagttgc catgatttac attgaacagc ttgaattaat
    69181 ccataaaagt ggtgatgtgt tgtatccagt aaaaataacc agaaaatcat caggaaaaac
    69241 tgccttccat cttgttccat tcggcttaaa taagactcac gaccttctag aggtggaaga
    69301 tgcttcagaa gcaattcggc tggtgattga tgaacgtcat tccattcgct gcagtacact
    69361 tacagcaact attacgaata aaaagggcaa gcgaattaag cggacaggta tatacagtat
    69421 caagggtgtt aacataaaag aatacaacgt caggtaatgg cacagagcgg tccgtcagtt
    69481 caggtttggc tctgtgccaa agatgggtca ggtcacacct gatctaataa atttaagcca
    69541 ttaaatcagc cttaaccttc accaccacct ttttaatctc acccggctcg ccgacaacgc
    69601 atcctggttt atgcggttca cttggcataa acacggcgaa cattcccggt tttaagatga
    69661 tggcttgctc gttatcaatg gtgctgcaaa gctgataatc atcctcatgg tggaactctt
    69721 cacactggcg cgcagtgcct gccatgccaa acagaatccg ttcctcaccg tttaataaca
    69781 gctggatatc aatgtattgc tcgtgcaatt ccgctttttt ctcgacgggc gattgagtgt
    69841 taaacgtcat gacattcata aagatattgt cgccctgtaa ttcgtaacga ccaggcgctt
    69901 tttcttgcgg tctggcagct aatgccagcg ttaacgcgtc ctgtaacgca ggatgtaacc
    69961 cagcagacgg taatgactgt acttcaccca tcatcataat ttttctccct gggccaacag
    70021 cgcagcccca agtaaacctg catcgtggcg gtagtgcgcc gccagtaaat caacatgaaa
    70081 tgccgctggc tcctgcgcca gatacgtttc caccaacgcc agataccctt ctgccagacc
    70141 aacgctgcca ccgaccacca cgcactggca atcagttgtg gctttaatat cagcgatcag
    70201 ccttgccagc acatgtgcgg agcggtgaat cagttgctgc gcctgctcgt cgccctgccc
    70261 ggcgcgtgtg aaaatagttc tcgcatccgc gcccatgagt tcaccttgcg ctgccgctgc
    70321 aataccgcga ccagaagcaa tcgcttccac gcaacctgtg cgtccacagc cgcagactgg
    70381 gccgtgtgga tcggcaagcg tatgcccgat gtgccccgcc agaccgccag ggccggtaag
    70441 cagtttaccg ccgctcacta caccgccgcc gacgccagtg gaaacggtga taaacaccat
    70501 atcggttata tcgccttcca gcgcctgata ctctgcccat gctgcggcct gcgcgtcgtt
    70561 aatggcaatg gtcggcaaat tggaaagttg ttccagcgtt ttgactaacg gaaagtgtag
    70621 caatccacca agattatgcg gattcagcgc cagcaagctg ccgtcgcgaa taattccggt
    70681 tgaagcgatg gcaacctgct gcgcatgagc ttgcaacggg gcgactaacg cggctaaggc
    70741 atcacgcaac gcctgcggag tctggctggc tggtgtcgga agttctcggc gatcgcggat
    70801 ctgcccgtca gcgccaatca gcgcggcggc aagtttagta ccgccgatat caatcgccaa
    70861 cgtggtcata gcaccgcctt tttcatcgct gtgttgtacc actgacaaat gtgttcaaga
    70921 cgcgtaatgg cagaaccgac cgtcaccgcc cacgcgccgt ggcgcatcgc atctgccgcc
    70981 tgagcaggcg tgttgtaacg cccttcggca atcacccgac atccggcgtc gctcaacgtt
    71041 ttcaccagcg ccagatccgg ctcttctggc gtttcaggcg tggtatagcc agaaagcgta
    71101 gtgccaataa tttcggctcc cagcttttgg catgccaggc cgtcttccgg cgttgagcag
    71161 tcggtcatcg ccagtaaacc gtgatggtga atacgtgcca gcagcgtttc aacaggcacc
    71221 ggacgcgggc ggtcggtgcc gtcaatggcg ataatgtccg cgcccgcctg cgccagcgca
    71281 tcaacatctt caatataggc cgtgatgcgt accggagaat cctccagatc gcgtttcaca
    71341 attccaataa tcggcacgct caccaccgca cgcgtggctt gcagatttgc cacaccttca
    71401 atgcgaatgg caaccgcgcc cgcctgttct gccgctaatg ccatggcagc gacgatttcg
    71461 ggtttatcga gcgggctgtc cggaaccggc tggcaggaga caatcaggcc accgttagca
    71521 gcgatttttt gatccagttg tgcaagtaac gacatacatc ttcccttagc gaaaggcccg
    71581 gtacagagac cgggcaacag gattaacttt tggttttgac taaatcgttt ttggcgctgc
    71641 caaatggcac ggcaccgcta aatggtttgc cgtcgatagc gtcatgagta cgcaatgctt
    71701 ccgggcgcag ccaacgctga acgcgggaag gcatatccag accaatcagc aggatcacca
    71761 caaatgtcag actgaacgat agcgatgcca gcgcagtacc cagatccaga cgttgagcga
    71821 tcaacgcgcc gatgattggg gccagtgcgc cgcccaatgc gccaacgttg taggtaaagc
    71881 ccaggcccgc tgcacgctgg tcggtatcga aataaccgcc aatcagtttc ggtaagatcc
    71941 cggcgatccc ttgtccaagc atttgctgga agaacagtaa cagaccgagc acccagacgt
    72001 ttgcgccgcc aatcgcaaat accggaataa tcaacagctg cgaggccaac aggctacaaa
    72061 cgtaggcttt gcgggttccc agccagtcac cgaggaagcc gctgacacag caccccaccg
    72121 cagccccaaa gccgctaaag aacagcacat tggcgacagt atgcgggtta taagccagat
    72181 cggttttcag atacgttggc agcagcgcct gaatcggcca tgagtagagg aaagcaaaca
    72241 acacgaccac catcagcatt acgcccgttg gccagcgttt acccgtactt tgcaccataa
    72301 agctgataaa gattgcggcg cataacagcc caagaacagc gacgatcgcg gcattttgca
    72361 ggttaccggc gaagcaaaac cacagcgcag tagccgccgc cagtgtcatt acgatattgg
    72421 caatgcgatg ttcaccacgg tagagaatat ctaccatcgt gcgtactggt gctttacctg
    72481 catgcttctc tttccagtct tccgcttccg ggatgttttt acgcagccag agagcaaaga
    72541 tgataggcaa aatgccgata aagaacagcg cacgccagcc ccagaccgga accaccaggc
    72601 tatagacctg agcggcaacg acggccccca cagagaagcc tgaaatcaga aaaccactgg
    72661 ctttgttacg cagatgtttt ggccagcttt caatgacata ggtggcgctg gaaccgtatt
    72721 cacccgccat ccccatgccg atgaccagac gcgcgataaa catggtgatg tagcctggcg
    72781 caaagccgca ggccagcgtc ccggccgaga agagaacgat gctggtgacc attgccagac
    72841 gacgcccgta gcggtcaccc atagcgccga gcatcaggcc gccgaaccag cgagagataa
    72901 aggctgcgga gatcagactt gccgcctgca ccgtcgtcag cccgaattca ccctgtactt
    72961 cggtgagtac cagggcgatt aaaacgaaat caaaaccgtc aagcagatat cccaaccagg
    73021 cagcggaaaa tgcgcgccat tgtgcacggt tgagatggcg ataccacggg atattctggg
    73081 ttgtagtact cattgtgagt ctcccgcggt gggcgatgcc cacacgcttt gctataaaaa
    73141 ttgtagggta cagatgcgtt tatttcccct cacccggtag gggcgagcga ggggaaacaa
    73201 ctcacccgcg ctcttgcatc aactgctggg ccagcgcctt cagttctggc agatattttt
    73261 catctaccgg tccaaacggt ttgcggcaca gcggcacaga aacgacatcc atataatgga
    73321 ggacagtttt caggccgcgg aatacgcccg ttttgatcag taaatcaatg actttattgc
    73381 attcagtttg cagtttctgc gcggtctgga tatcgccttc tttcagcgcc ttaacgatcc
    73441 cctgatagcg ccagcccatg atgttgtagg tactaccgat accaccatca gcgcccgcca
    73501 gcagaccaga ggcgaagatt tcgtcgtaac cgttatagag cacaagatca ggatgttcac
    73561 gacggatctg ctccatctga tagagatcgc cagaggtctg tttcagcgca cctacgccag
    73621 gcaatgtaac aagtgtgttg atctgatcca gggtcagttt taccccactc agggctggaa
    73681 tgttgtacac caccatcggc aaaccatccg ccgaatcaat aattgcccga tagtgatcgc
    73741 agtgttcttc aaagctgaaa ggatagtaga acggcgtgac ggcggagacg gcatcgaagc
    73801 cataacgttt agccgatgcc gcaagttgtt ggctttcggc ggtgctgacg caaccgacgt
    73861 gggcgatgag tttaatctta cctttcgcct cttcggcgac gatttccagt acctgttcac
    73921 gctcggaaag gctttgtaca aaggcctcgc cggtcgaacc acccacgtat aaaccgtcga
    73981 tgccctgctg aatattgaac tgaaccaggc gacgcagact cgctttatcc agtgcttgtt
    74041 gttggtcaaa aggagtcagg agtgcagcca ttacgccacg taaattcgtt gccataaata
    74101 cctctgaagt gatgcttgtc tgataaacga tataccttta tacctgttat accagatcga
    74161 ttaagcaaca ccccatacag aaagcttata atgcgatctg cttcactaaa gtggcattat
    74221 ttctttttgt tggtggtctg accgaaagcg tgccaggtag cagagacgct gttgagatgc
    74281 gattgcaacg cacgatcggc ttcgtcagga tcatgacggc ggatcgcatc aacgatcgca
    74341 atatgctgtt gataactaac gttgttatgt tcgtgcagtg cctgatcggt aaccgttggg
    74401 cgtgcggcaa taagccagtc gagcagggca acgtggatcg ccatgaagat tgggttaccg
    74461 gggatctccg ccagcacgcg gtggaaatca acgtctgaac gaatgaatgc cgcgttgtta
    74521 tccagcgact gactgttgat ttccagtgct tttgccagca aatcgatttg ctcatcggtg
    74581 gcatgttcag ccgcatagcg caccagactg gattcaaaga acagacgtaa ttgttcgaaa
    74641 tgggcaatcc caccgggatg agaaaggaaa tctttcgcca tgccggaaag ctcaccgatg
    74701 atagtgtccg cagaaggacg cgagacgcga gcgcgttcgc cgttgtttat ttgcaccaga
    74761 cctttgcgtt ttaacgctgc cagcgcttca cgcaccgaag gacgcccgac gttaaagaac
    74821 gccatcagtt cgcgttcaga cggtaattgt tcaccttcgc caaattcacg acggcggatc
    74881 atctgttcca gctcttcttc caccatttcg gagagttttt tacgcgccag cgggcggcta
    74941 cgcaagttgc gaccaattgc aggtgaagaa tcttcggttt gcgaatcaaa tgcgttcata
    75001 aggcccattc tgtaaggtca gtgtgattaa catcatcagt gacatcctat cacaggattg
    75061 agagtagggg aaaatggcag gtttttctct ttgtgcctca tcattaccat aattaacgga
    75121 ataattaact attgcgaaaa attaatgtaa cgcagataaa aacatcccgt ttgaattatt
    75181 tataagacta ttcacgagca ttatgaatat tatgaatgcg ttcttgcaaa ataatcataa
    75241 gcgcatattt tttaatgaaa aattatctca cctacaatta aaaacacgac atccgcacca
    75301 taaatagact tgcaaaaaat ataacatcat tgttttcaat ctgccgttta tgggattgac
    75361 cgttttcttt tgacacggag ttcaacaatg ttcggcataa ttatatctgt catcgtatta
    75421 attacgatgg gctatttgat cctgaaaaac tacaaacctc aggtggtgct ggctgccgca
    75481 ggtatcttcc tgatgatgtg cggtgtctgg ttagggttcg gtggtgtact cgagcccgcc
    75541 aaaagcagcg gctacttgat cgtcgatatt tataatgaaa tcctgcgcat gctgtccaac
    75601 cgcattgccg gattggggct atcgattatg gcggtgggcg gtatgcccgc tacatggagc
    75661 gcataggggc cagtcgcgcg atggtgagct tgttaagccg cccgttaaaa ctcattcgct
    75721 caccgtatat tattctgtcg gcaacttacg tcatcggtca aatcatggcg cagtttatta
    75781 ccagcgcctc cggtctgggt atgttgctga tggtcacctt atttccgaca ctggtgagtc
    75841 tgggagtaag tcgtctctct gcggtggcgg ttatcgcaac cacaatgtcc attgagtggg
    75901 gaattctgga aacgaactcc atttttgcag cccaggtcgc gggaatgaaa attgccactt
    75961 acttcttcca ctaccagctt ccggtcgcct cttgcgtcat tatctcggtg gcgatctccc
    76021 actttttcgt gcaacgcgct tttgacaaaa aagataaaaa tatcaatcac gaacaagcag
    76081 agcaaaaagc tctcgataat gtcccgccgc tctattacgc cattttacct gtgatgccgt
    76141 taatcctgat gctcggctcg ctgttcctcg cccacgtcgg gctgatgcag tcagaactgc
    76201 atctggtggt ggtgatgtta ctgagtttga cggtgacgat gtttgttgag ttcttccgca
    76261 agcataactt gcgcgaaaca atggacgatg tgcaggcgtt ttttgacggc atgggtacgc
    76321 agtttgccaa cgtggtaacg ctggtggtcg cgggtgaaat atttgcgaaa ggcttaacga
    76381 cgattggcac tgtcgatgcg gttatcaggg gtgcggagca ttctggtctg ggcggtattg
    76441 gcgtgatgat tattatggcg ctggtcattg ccatttgtgc cattgtgatg ggctctggca
    76501 atgcgccgtt tatgtcattt gccagtctta ttccgaatat cgcagccgga ctacatgtac
    76561 cagcggttgt aatgattatg ccgatgcatt ttgccacgac gctagcgcgc gcggtttcgc
    76621 cgattactgc ggtggtggtc gttacgtcag gaattgcagg cgtttcgcct tttgcggtgg
    76681 tgaagcgaac agcgatcccc atggcagtcg gtttcgtggt gaatatgatt gccacaatca
    76741 cgctatttta ttaagccatt aaaagacaaa acaggccacc agggcctgtt tttgtattac
    76801 ttcacaacgc gtaatgccgg tcgaccaccg cgtggtggct gcggaggttc atcgtcagga
    76861 tgagtgtcat catcgtgatc tggcttgtcg ccatcaataa ccgacataac ggtttcgttg
    76921 tctgccgatg cctcttcatc attcatgatg ctggtatctt catcgtaggc agcttcaggc
    76981 tcaaacattg tgcctgcgcc attttcacgg gcgtagatag ccagcacggc agccagcggc
    77041 acagaaacct gacgcggaat gccgccaaag cgcgcgttaa agcgcacctc atcattcgcc
    77101 agttccagat tgccgacagc acgcggcgca atgttgagta cgatttgccc gtcacgcgca
    77161 tattccatag gaacctgcac gccagggagc gtcacatcca ccactaggtg cggcgtgagc
    77221 tggttatcca gcaaccactc atagaatgca cgcagcagat agggacgacg tggtgttagc
    77281 tgtgacaaat ccatacagat taactccggc ccagacgcat ttcacgttct gcttcagtta
    77341 aagaagcaag gaaagagtca cgctcaaaga cgcgggtcat atagcctttc agctctttcg
    77401 cacccgggcc gctgaactcg atgcccagtt gcggcagacg ccacagcagc ggagcaagat
    77461 agcaatcgac caggctgaac tcatcgctca ggaagtacgg cttctgaccg aagaccggcg
    77521 caatcgccag cagttcttcg cgcagttgct tacgtgcggc atctgcttca gaagctgaac
    77581 cgttgatgat ggtgttcatc agcgtgtacc agtctttttc gatgcgatgc atgtacagac
    77641 ggctttcacc gcgagctacc gggtaaacag gcatcagtgg cggatgcggg aaacgctcat
    77701 ccagatattc cataatgatg cgagattccc acagggtcag ctcacgatcc accagggtcg
    77761 gaacgctctg attcgggttg aggtcaatca gatcctgagg cggattgtcc ttttccacgt
    77821 gttcgatctc gaaacttaca cctttctcag ccagcacaat gcggacctga tggctataga
    77881 tgtcagtagg accggaaaac agcgtcatta ccgaacgttt gttggcagcg acagccatga
    77941 aaacctccag gtatagtcag aatttttact gctaccagcc accaggtggc cagtcagaag
    78001 ttgtgttacc cagtaaggaa cgactctctt tgttcgaaaa tcaaacaaaa aatgagcaat
    78061 acccgacatt tgggcagaaa attggatgat agtttaccag attttgcgac ctttgtggtg
    78121 agtcgatgcc ggaaatgggg aaaaagagat gcgctttagt ctgaaatagt tgacttagtc
    78181 ccttattggc gatgtggttt ttgttttacc tgtctgtcag gtggcagcaa aaagcaactt
    78241 tccagttttt acgctgattc agattttagc tataaaaaaa cccgccgaag cgggtttttt
    78301 cgaaaattgt tttctgccgg agcagaagcc aattaacgtt tggagaactg cggacgacga
    78361 cgtgctttac gcagaccgac tttcttacgt tcaacctgac gagcgtcacg agtaacgaag
    78421 ccagctttac gcagttcaga acgcagggac tcgtcgtatt ccatcagagc gcgggtgata
    78481 ccgtgacgga tcgcaccagc ctgaccagag ataccaccac ctttaacggt gatgtacagg
    78541 tccagtttct caaccatgtc gaccagttcc agcggctgac gaactaccat gcgggcagtt
    78601 tcacgaccga agtactgttc cagagaacgt tggttgatta cgattttacc gttgcccggt
    78661 ttgatgaaaa cgcgagctgc ggaacttttg cggcgaccag tgccgtagta ttgattttca
    78721 gccattgcct ataatcccga ttagatgtca agaacttgcg gttgctgtgc cgcgtggttg
    78781 tgctcgttac ccgcgtaaac tttcagttta cggaacatag cacgacccag cgggcctttt
    78841 ggcaacatgc ctttaaccgc gatttcaatc acacgctcag gacggcgagc aatcatctct
    78901 tcaaaggtcg cttgtttgat accaccgatg tggccggtgt ggtgatagta cactttgtca
    78961 gtacacttgt tgccggttac agcaactttg tcagcgttca gaacgatgat gtaatcaccg
    79021 gtatctacgt gcggagtgta ttccgctttg tgcttaccgc gcaggcgacg agccagttca
    79081 gtagccagac ggcccagagt tttaccggtc gcgtcaacaa cataccagtc gcgttttacg
    79141 gtttctggtt tagctgtaaa agttttcatt aaaagcttac ccaataaata gttacacgtt
    79201 ggtgaacacc caaacgtctt caattgttga ggttcacacg acaaagtccg gcaaacctac
    79261 cccttcgaat agcctatgcc agcacacaaa aagttttggg aaaaaacttt cttgtaacgt
    79321 ggggtcgcag gattatagag aagtcggggt caaagatcga cccctttttg tgatttgtga
    79381 caggttttaa cccgccaaat gctcgcgctt cagatactct tcgctttgca tctcttgcag
    79441 acgtgacagg caacgctgga actcaaactt cagccgatcg ccctgataaa tttcatacag
    79501 cggcacttct gcactcacca ctaatttgac atggcgctcg taaaactcat ccaccagcgc
    79561 aataaagcgc cgcgcttcgc tctccatcaa ccgcgtcata actggtacat caaacaacat
    79621 gaccgtatga aagaggcgtg agagcgcaat atagtcatgc tggctgcggg catcgacaca
    79681 caaggtggta aaagagacag caagcgtctg attctcagcg cccattgtcg ccagcggtcg
    79741 atggttgatt tctaacgtcg gtgaattttc gcgtttcgct cccgccagcg ccaaccatag
    79801 tttatccatt tgtgcccggg tttcatcgtg gagtggcgaa agccacagat gcgcctgagt
    79861 gagtgtacgc agtcgataat caacaccagc gtccacgttc attacatcac aatgctgttt
    79921 aatggcatcg attgcaggca gaaaacgcgc acgttgcaag ccatttcgat aaagttcgtc
    79981 cggcggaata tttgacgttg ctaccagggt aataccgcgg gcgaacaggg ctttcatcag
    80041 accgccaagt agcatggcgt cggtaatatc agaaacaaaa aattcgtcaa aacagagcac
    80101 gtcagtttcg gctttaaagc gatcggcaat aatttccagc ggatcggtct gcccctgtaa
    80161 ggcagttagc tcttcgtgca cccgcagcat aaaacggtga aagtgcaggc gctgtttccg
    80221 ctctcccggc aggctttgat agaaaaggtc catcagccag gttttcccgc gtcctacacc
    80281 gccccacata tataagccac gcactggcgt atgctttgtg tcttcgcgtt taccccacag
    80341 cttaccgacc cgcgccatta gcccactcgt cctgggggct ggtggcctgc tattgatgag
    80401 ttcttgataa ataatttcca ggcggctgac ggcctctttt tgaacgtcgt cgggttgatg
    80461 gctgccttca ttgagcgcct tcaggtattg cgatgttggg gtaacgcttt gcatgatctt
    80521 attattattc cttgaataat cggtgcgccg ttgttcacgg ttgacgaaaa aaaggccgtt
    80581 ctacactacg cgatatgcag tcgggattcc acttctgtgg aattaacggt tatagtggca
    80641 taatcagccg taggcatgga gcctgaagcc aacaccctac ggaaacaaaa gacaacggga
    80701 gatgttcatg acctgggaat atgcgctaat tgggttagtc gtcggcatca ttattggtgc
    80761 tgtggccatg cgttttggta atcgtaaact acgccagcaa caggcgttgc agtacgaact
    80821 ggaaaagaat aaagctgaac tggacgagta tcgcgaagag ctggttagcc actttgcccg
    80881 cagcgcggaa ttactggata ccatggcgca cgactatcgc cagctgtatc agcacatggc
    80941 gaaaagctcc agcagcctgc tgccggaact gtctgctgaa gcaaacccgt tccgtaatcg
    81001 tctggcagag tctgaagcca gcaacgatca ggcaccggtg cagatgcctc gcgactattc
    81061 tgaaggtgca tccggcctgc tgcgtactgg cgcgaagcgc gactaattta tttttcgggc
    81121 gcagccattg cgccctcctc ttctctccct ccccgactat catttaatct ggtgtctcat
    81181 tgttagccgt ctgaaaattc aataacatca aactgttttg aatctctttt cttatcattc
    81241 aggtacgaga gcaggaataa tgaaaaaaca aacccagctg ttgagtgcat tagcgttaag
    81301 tgtcgggtta actctctcgg cgtcatttca ggccgtcgcg tcgattccag gccaggttgc
    81361 cgatcaggcc cctctcccca gtctggctcc aatgctggaa aaagtgcttc cggcagtggt
    81421 gagcgtacgg gtggaaggaa cggccagtca gggacagaaa atcccggaag aattcaaaaa
    81481 gttttttggt gatgatttac cggatcaacc tgcacaaccc ttcgaaggtt taggttccgg
    81541 tgtcatcatc aacgccagta aaggctatgt gctgaccaac aaccatgtga ttaatcaggc
    81601 acagaaaatc agtattcagc tcaatgatgg gcgcgagttt gatgcaaaac tgattggtag
    81661 cgatgaccag agcgatatcg ccctgttaca aattcaaaac ccgagcaaat taacgcaaat
    81721 cgctattgcc gactccgata aattgcgcgt cggtgatttt gccgtagcgg tcggtaaccc
    81781 atttggcctt gggcaaaccg ccacctctgg cattgtttcc gcattaggcc gcagcgggtt
    81841 gaatcttgaa ggtctggaaa actttatcca gacagatgct tccattaacc gcggtaactc
    81901 cggcggtgca ctattaaacc ttaacggtga gttaattggc atcaacactg caatccttgc
    81961 gcctggcggc gggagcgtcg ggattggatt tgccatcccc agtaatatgg cgcgaacact
    82021 ggcgcagcag cttatcgact ttggtgaaat caaacgcggt ttgttaggca tcaaaggcac
    82081 cgagatgagt gccgatatcg ccaaagcctt caaccttgac gtgcagcgtg gcgcgtttgt
    82141 cagcgaagtg ttgccaggtt ctggctcggc aaaagcgggc gtcaaagcgg gcgatattat
    82201 taccagcctc aacggcaaac cgctgaatag ctttgctgag ttgcgctctc gtatcgcgac
    82261 tacagagccg ggcacgaaag tgaagcttgg cctgctgcgt aacggcaaac cactggaagt
    82321 agaagtgacg cttgatacca gcacctcttc gtcggccagc gctgaaatga tcacgccagc
    82381 gctggaaggt gcaacgttga gcgatggtca gctaaaagat tgcggcaaag gtattaagat
    82441 cgatgaagtt gtcaaaggaa gcccagctgc tcaggcaggc ttgcaaaaag acgatgtgat
    82501 cattggcgtc aaccgcgatc gggtgaactc gattgctgaa atgcgtaaag tgctggcggc
    82561 aaaaccggcc atcatcgccc tgcaaattgt acgcggcaat gaaagcatct atctgctgat
    82621 gcgttaatgt cgtaaaccgg gcatcaggct tacgtgtgat gtccggttaa ctcgtggtat
    82681 gctgctgccg ttcccttttt taatgacgcc tccatcatgt ttgtgaagct cttacgttcc
    82741 gttgcgattg gattaattgt cggcgctatt ctgctggttg ccatgccttc gctgcgcagc
    82801 cttaacccgc tttccactcc gcaatttgac agtaccgatg agacgcctgc cagctataat
    82861 ctggcggttc gccgcgccgc gccagcggtg gttaacgttt acaaccgtgg tgtgaacacc
    82921 aactctcaca accagcttga gatccgcacc ctgggatccg gtgtaatcat ggatcaacgc
    82981 ggttatatca tcaccaataa acacgtcatc aacgacgccg atcagatcat cgtcgcctta
    83041 caggatggac gtgtatttga agcattgctg gtgggatctg actctctaac cgatctggcg
    83101 gtacttaaaa ttaatgccac tggcggttta cctaccattc caattaatgc acgtcgcgta
    83161 ccgcacattg gcgacgtagt actggcgatc ggtaacccgt acaacctcgg gcagaccatt
    83221 acccaaggga ttattagtgc cacggggcga atcggtctga accctaccgg gcggcaaaac
    83281 ttcctacaaa ccgatgcttc cattaaccac ggtaactctg gcggcgcgct ggtgaactcg
    83341 ctgggagaac tgatgggcat taacacgctg tcgtttgata agagtaacga tggcgaaacg
    83401 ccggaaggta tcggctttgc gattcctttc cagttagcaa ccaaaattat ggataagctg
    83461 atccgcgatg ggcgcgtgat ccgcggctac attggcattg gtggacgtga gatcgcacca
    83521 ctgcacgcgc agagcggtgg tatagatcaa ctgcaaggga tcgtggttaa tgaagtgtca
    83581 cctgacggcc cggcggcgaa tgcgggtatt caggtcaacg atctgattat ttcggtggat
    83641 aacaaaccgg ccatctctgc tctggagacg atggatcagg tggcagaaat tcgccctggt
    83701 tcggtgatcc cggttgtagt gatgcgtgat gataagcagt taacgctgca ggtcaccatt
    83761 caggaatatc cggcaaccaa ttaagtcgag tgctcaaaac aaaaaaccgg agtctgtgct
    83821 ccggtttttt attattcgct aatcaattac ttattaacga actcttcgcc cagggcgata
    83881 tctttcttga gcgtatccag cataccttcc agcgcgttct gttcaaatgc gctcagggta
    83941 ccgatagatt tacgctcttc cacgccgttt ttacccagca gcagcggttg agagaagaaa
    84001 cgggcgtact gaccgtcgcc ttcaacgtag gcacattcga caacgccttg ttcgccctgc
    84061 agtgcacgaa ccagagacag accaaaacgt gcagctgcct ggcccataga cagggttgca
    84121 gacccgccac cggctttcgc ttcaaccacc tcagtacccg cgttctggat acgtttggtc
    84181 agatcagcca cttcctgctc ggtaaaacta acgccaggaa cctgtgacag cagcggcaga
    84241 atggtaacac cagagtgacc gccaataacc ggcacttcaa cttcgcttgg ctgtttgcct
    84301 ttcagttccg caacaaaggt gttggaacga atgatatcca gcgtggtaac gccgaacagt
    84361 ttgtttttgt cataaacacc ggcttttttc agcacttcag cagcaattgc aactgtggtg
    84421 ttaaccgggt tagtgataat accaatgcac gctttcgggc aggttttcgc aacttgctgt
    84481 accaggtttt tcacgatgcc ggcgttaacg ttaaacaggt cggaacgatc catacccggt
    84541 ttacgcgcta cacctgcaga gataagaacg acatctgcgc cttccagcgc cggagtcgca
    84601 tcttcaccag aaaaaccttt gattttcaca gcagtaggga tatggctcag atcgacagcc
    84661 acaccgggag tcactggagc gatatcatac agagagagtt ctgaacctga aggcagttgg
    84721 gtttttaaca gtagtgcaag cgcctggcca ataccgccag cagcgccgag gactgcgact
    84781 ttcatcctaa actccttatt atattgataa actaagatat gttgctccgc tgccgcgacc
    84841 ttaatccaca aaattgccgt ttacaatgac cacatatcaa gaatgtgtag tcacgcaagt
    84901 ttagcgttta tgcatttaat tgccgtaatc aggaacctaa cttacgtaat taacagtcgc
    84961 ttatgcatta gcgcaacatt ccaacaggtg gtgacaatat accctaccgt tcagccaaaa
    85021 caacatcaat ttgataacaa ttaatttact tttaagcaga atttgcatgc cgtgacgcag
    85081 gcatgtttct caataacgaa atttgataaa atcccgctct ttcataacat tatttcagcc
    85141 ttcttcaggg ttgactgttt gcataaaaat tcatctgtat gcacaataat gttgtatcaa
    85201 ccaccatatc gggtgactta tgcgaagctc ggctaagcaa gaagaactag ttaaagcatt
    85261 taaagcatta cttaaagaag agaaatttag ctcccagggc gaaatcgtcg ccgcgttgca
    85321 ggagcaaggc tttgacaata ttaatcagtc taaagtctcg cggatgttga ccaagtttgg
    85381 tgctgtacgt acacgcaatg ccaaaatgga aatggtttac tgcctgccag ctgaactggg
    85441 tgtaccaacc acctccagtc cattgaagaa tctggtgctg gatatcgact acaacgatgc
    85501 agttgtcgtg attcatacca gccctggcgc ggcgcagtta attgctcgcc tgctggactc
    85561 actgggcaaa gcagaaggta ttctgggcac catcgctggc gatgacacca tctttaccac
    85621 ccctgctaac ggtttcaccg tcaaagacct gtacgaagcg attttagagc tgttcgacca
    85681 ggagctttaa tctctgcccc gtcgcttctg gcggcgggga aaatgttgct tatccctctc
    85741 aaccccctgc tttcccctgc gattaattta acgaatagtg cgttttactg cgacatgtca
    85801 ttcacataat gaatacatac ggtaaaaaaa gcacattatg caaaattcat tatctaattg
    85861 aaaatactag aattaaaaat aaatgaccgt gtttttaatt cttttttgtt attaaaattc
    85921 acatttttaa cacttagtat caactgaaac agttagcgtg gtattaatta gttcaataat
    85981 tagtgtatgc ttgattttgt gatatgggtc acaaaacaaa ggcccagcta aaagattatg
    86041 tcgaggtaaa aatcatgaaa atcaaaacca ctgtagctgc attaagcgta ctttctgttc
    86101 tctctttcgg tgcattcgct gccgactcca ttgatgctgc acaagcacaa aatcgtgaag
    86161 caatcgggac cgtatccgta agtggtgtgg cgtcttcgcc aatggatatg cgtgaaatgc
    86221 tgaacaaaaa agcggaagag aaaggcgcaa cggcctacca gattactgaa gctcgtagcg
    86281 gtgacacctg gcacgctacg gctgaactgt acaaataaac cctcatcgtc ttgtccgacg
    86341 atattgcccc cggttcgggg gctttttttg cgctaatgac gaacattaaa acgcaaatgc
    86401 ccttccagct cttcctctgc ctcatcaaac agcaatatta acgcgccaaa acggcgacgc
    86461 gttttctctc ccagatgaac aaattcaatc tcaagtggta gcggcaggac atcgttcatt
    86521 aacacatccc atagtgagtc gagatcgcgt accttatctt tcgccagacc aaaggtttgg
    86581 ctaaagtcac gataaaaatc ctcctgactc tcaatctcat caaaatcaaa ggtataaata
    86641 ttcatctatt gccaccgtca cgtttcgcgg cgagttctgc cgtgcatcgc taagtatagc
    86701 catgaaaaaa ccgacacttt tggcgtcggt tttgacctaa ctatcggtca acgcatgttg
    86761 atacttatgg agcatacccg ccagccgatg aaccggttcc gtcacctgcg gtggcgcttg
    86821 ccagatgcgt agcttttcct ggtagatttc aagttcttcc agcaactggc caaagtaccg
    86881 acgacgttta tcatcgctac gggcagatat cacatggtct gctgtgcggc gcatttgtcg
    86941 gtgaaacgcc gataaatcct cgttaaccgg gatcggcgca tcacgcaggc gctggtgcgc
    87001 gataatcatc gtcagcgcca ggcgaaattt cggcaaatcc cctgggaact tattcatcag
    87061 caaaaacagc tgctgataaa gtgccgggag gtggttctct ttacgacgtg ccacattggt
    87121 agtcatcgcg gaaacagcgg cagaaacaaa ctgattaagc agtacacgtt cggtcctgtc
    87181 gcgcgattta tcccgcacaa gcaaaataac ggtgaacgcg agcacacagc cgacgatttg
    87241 ccctaatgcg ctgtcgagaa actgactgaa atggaaagtc atcgggttat ccagcacgat
    87301 aatatttatg gtgctggcca gcgcccccat cgagcccagt cgccgttgct gtacttctat
    87361 accgaggaaa aatcccagca ctgccaggct aatacacagc agcaacatgc tctgttgggt
    87421 attagggata atcaccaaaa agtagagcag ccctaacggc agcgcggcca gcgtcccgta
    87481 gataaagtcg atcgccacca tgcgtggatt cggcaaacgc attgccagtg acgtcactac
    87541 cgcaatcatc accattgcac cactgccgga agtccagccc gtccacagcc agaaaagcgt
    87601 gcccagaatg caggaaagtg tggttcgcca gaagttaacc atcgcatgat gacgttcggc
    87661 tgactctact ttgacttccg gttcgccttg caggatctct tcttcggtgg cgttgatttt
    87721 tgtgttactg ataacgccgc gcttgagaag ctgataacgc gttgccgccg cgacccagct
    87781 ataaatggtg acaggcgttt cccgttcccc ggtccaggcg ataactctcc gcaggcgttt
    87841 gagctgcttg tggacgtcct gcgcggtttc taccggcgtg tcaaaaaatt cgcggaaagt
    87901 atcagtgatc aattccgggc gcgtattctg aataagataa gtttcgcagg attgggtaat
    87961 cagcgtcagc gatagcgtat tgatcgcttt taaacgtcga ttggcccgcg cccagcggga
    88021 agattccata ttcaggttgc tgcgcatgcc ttgtagcgcc gtggtgcgtc gcaccaggtc
    88081 gccccaggct ttatcgacaa cttcaccatc gccatgcttg atacagagtt gcattaattg
    88141 atattgcgcg accagcaaac tttccagctc tcgatccact tcttgtttga tcgatcgcgg
    88201 agaaaagagc aaatccgcta taatcgcaca cacaataccg ataacgatct cgctacaacg
    88261 ttcgacggca aactgcggcg taagcaatgg ttccggctga atggtgatca caatgatcag
    88321 cgcggtataa ccggccagcc cccacgcgta cgagttttct attcgtacca gcgaggatat
    88381 ccaggtacaa aaaccggccc agatacagca caccagaatc atcaataatg gtgcgcggat
    88441 catcgcaatg atgatcacca gtccggcaat acagccaata aatgtgccga tgatgcgcaa
    88501 aaagccacga taacgaatag cgccagaata cggttcacct cccgcagcaa aggccggacc
    88561 ggcggcaaca atcgccgctg tcagtaccgc ccagcgtggc gtttccagct ggaagtgaaa
    88621 gccaacaaac agcgccagta caatggcggt cgccagtttt accgcaaagc gaatatgttg
    88681 gttagcaatg gagaaaatac ccatcgtgat taaccaaact cacgcaggcg atgggccatt
    88741 ttacggaaga acgaatcctg gctttcgtcg cgatcttgtt tgccagtgac caccactgta
    88801 gcagtggtgc ccgcaggcca gatgttctct tgctggttgt cgagacgaat acgaaccgga
    88861 acacgttgcg caagacgcac ccattcaagg ttagagtcta tagtcgccat ccctttgtcg
    88921 tcacgcgtgc tgctggcgtt ggtgacccct gcggcaacac tatcaacagt ccctttcagc
    88981 actttgttac tgccaagcgg cgtgatctct gcacgatacc ccggacgcac cccttccagc
    89041 ttagtttctt ccatataggc cagtacatag aaggagttct gtttcaccag cgcaaccgcc
    89101 gttgatcctc gagtaataaa ctcaccggta tagacgttga ggttggtcac ccagccatct
    89161 gctggcgcgc ggatcaccgt gcgttcaaga tccagttttg ccagatcgcg ggtcgcctgc
    89221 gctttcgcta actgatgcag aaccgtttgt agtacgttgt tagcctagtc gatctcttcg
    89281 cgagacatcg cctgcacacc gagacggtta cgacgtccgg cctcctggcg tttctcctgt
    89341 gccagtacct gataataagc aacatcggct tgcgcttcct caagcgcctt ttgatagcgc
    89401 ggctggtcga tggtgaacag tatctgtcct tttttcacca gctggttatc atgaacattc
    89461 acctgggtaa tgagtccaga aacgtccggc gcgatcgcaa tgacgtcagc gctaaagcgc
    89521 gcgtcacgcg tccaggggga ttcggtgtaa tagacccagg cattaaaaat tgcgatgaag
    89581 gccagaatga ctaatacgac cgtgatggcc gtacgggaga attttcttat tagtgttttc
    89641 acttcaacct caaacgaaca gtcgcgatat caaataaaac aagcagcaat agagcgcggt
    89701 gttgaacaac gccggatgcc agacaaagtc gtagatacct gttggcacaa gtacccggcg
    89761 caccagccag aaaatcgcca gtgataaaag caattcaaaa aatatcggtg ggaaggacag
    89821 cccaaacacc acgataacgg gaaacagact catgttgacc ttggttgtat agagagagca
    89881 ggcgttatta ttttcagcat ctgtcgccgc agagaagggc atggaaagcc gggcgagagc
    89941 aacattgctg tagattgata tttaatatat tagcgtaact gttatgctgt tatcta
//
LOCUS       DAJTMC010000002        80833 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.002, whole genome shotgun sequence.
ACCESSION   DAJTMC010000002 DAJTMC010000000
VERSION     DAJTMC010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 80833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 80833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..80833
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.002"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            414..1616
                     /gene="proV"
                     /locus_tag="OO130_000095"
     CDS             414..1616
                     /gene="proV"
                     /locus_tag="OO130_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HCR5363652.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            1609..2673
                     /gene="proW"
                     /locus_tag="OO130_000096"
     CDS             1609..2673
                     /gene="proW"
                     /locus_tag="OO130_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HCR5363653.1"
                     /translation="MADQNNPWDTTPAADSAVQSADAWGTPATAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQVLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPSIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            2731..3723
                     /gene="proX"
                     /locus_tag="OO130_000097"
     CDS             2731..3723
                     /gene="proX"
                     /locus_tag="OO130_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HCR5363654.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTLLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKEPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWTEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAALK"
     gene            4015..5199
                     /locus_tag="OO130_000098"
     CDS             4015..5199
                     /locus_tag="OO130_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5363655.1"
                     /translation="MTKPNHELSPALIVLMSIATGLAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISDKILRPRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            5323..6060
                     /gene="ygaZ"
                     /locus_tag="OO130_000099"
     CDS             5323..6060
                     /gene="ygaZ"
                     /locus_tag="OO130_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HCR5363656.1"
                     /translation="MESPTPQPALGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            6050..6385
                     /gene="ygaH"
                     /locus_tag="OO130_000100"
     CDS             6050..6385
                     /gene="ygaH"
                     /locus_tag="OO130_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HCR5363657.1"
                     /translation="MSYEVLLLGLLVGVANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            6476..7006
                     /gene="emrR"
                     /locus_tag="OO130_000101"
     CDS             6476..7006
                     /gene="emrR"
                     /locus_tag="OO130_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HCR5363658.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            7133..8305
                     /gene="emrA"
                     /locus_tag="OO130_000102"
     CDS             7133..8305
                     /gene="emrA"
                     /locus_tag="OO130_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HCR5363659.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTVREISLAPVNKLIDDIVKANAG"
     gene            8322..9860
                     /gene="emrB"
                     /locus_tag="OO130_000103"
     CDS             8322..9860
                     /gene="emrB"
                     /locus_tag="OO130_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HCR5363660.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWECGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(9924..10439)
                     /gene="luxS"
                     /locus_tag="OO130_000104"
     CDS             complement(9924..10439)
                     /gene="luxS"
                     /locus_tag="OO130_000104"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HCR5363661.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGSHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(10589..12145)
                     /gene="gshA"
                     /locus_tag="OO130_000105"
     CDS             complement(10589..12145)
                     /gene="gshA"
                     /locus_tag="OO130_000105"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HCR5363662.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTQATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGTDAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLPQVGKDLFRDLKRVAQTLDSINGGKAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHA"
     gene            complement(12218..12646)
                     /locus_tag="OO130_000106"
     CDS             complement(12218..12646)
                     /locus_tag="OO130_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5363663.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISYPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(12643..13209)
                     /gene="yqaB"
                     /locus_tag="OO130_000107"
     CDS             complement(12643..13209)
                     /gene="yqaB"
                     /locus_tag="OO130_000107"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HCR5363664.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDVQAMI
                     ALNGSPTWRIAQAIIELNQADLAPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            complement(13357..13433)
                     /locus_tag="OO130_000108"
     tRNA            complement(13357..13433)
                     /locus_tag="OO130_000108"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13397..13399),aa:Arg,seq:acg)
     gene            complement(13632..13708)
                     /locus_tag="OO130_000109"
     tRNA            complement(13632..13708)
                     /locus_tag="OO130_000109"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13672..13674),aa:Arg,seq:acg)
     gene            complement(13906..13982)
                     /locus_tag="OO130_000110"
     tRNA            complement(13906..13982)
                     /locus_tag="OO130_000110"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13946..13948),aa:Arg,seq:acg)
     gene            complement(13986..14078)
                     /locus_tag="OO130_000111"
     tRNA            complement(13986..14078)
                     /locus_tag="OO130_000111"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14042..14044),aa:Ser,seq:gct)
     gene            complement(14394..14579)
                     /gene="csrA"
                     /locus_tag="OO130_000112"
     CDS             complement(14394..14579)
                     /gene="csrA"
                     /locus_tag="OO130_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HCR5363665.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(14814..17444)
                     /gene="alaS"
                     /locus_tag="OO130_000113"
     CDS             complement(14814..17444)
                     /gene="alaS"
                     /locus_tag="OO130_000113"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HCR5363666.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKDANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEVAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(17572..18072)
                     /gene="recX"
                     /locus_tag="OO130_000114"
     CDS             complement(17572..18072)
                     /gene="recX"
                     /locus_tag="OO130_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HCR5363667.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYDEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(18315..19376)
                     /gene="recA"
                     /locus_tag="OO130_000115"
     CDS             complement(18315..19376)
                     /gene="recA"
                     /locus_tag="OO130_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HCR5363668.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(19456..19953)
                     /gene="pncC"
                     /locus_tag="OO130_000116"
     CDS             complement(19456..19953)
                     /gene="pncC"
                     /locus_tag="OO130_000116"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HCR5363669.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMVIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(20098..21183)
                     /gene="mltB"
                     /locus_tag="OO130_000117"
     CDS             complement(20098..21183)
                     /gene="mltB"
                     /locus_tag="OO130_000117"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HCR5363670.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTRYSISQLATAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            21439..22002
                     /gene="srlA"
                     /locus_tag="OO130_000118"
                     /gene_synonym="gutA"
     CDS             21439..22002
                     /gene="srlA"
                     /locus_tag="OO130_000118"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HCR5363671.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSQGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLALSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            21999..22958
                     /gene="srlE"
                     /locus_tag="OO130_000119"
                     /gene_synonym="gutE"
     CDS             21999..22958
                     /gene="srlE"
                     /locus_tag="OO130_000119"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HCR5363672.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            22969..23340
                     /gene="srlB"
                     /locus_tag="OO130_000120"
                     /gene_synonym="gutB"
     CDS             22969..23340
                     /gene="srlB"
                     /locus_tag="OO130_000120"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HCR5363673.1"
                     /translation="MTVIYKTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFTLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            23344..24123
                     /gene="srlD"
                     /locus_tag="OO130_000121"
     CDS             23344..24123
                     /gene="srlD"
                     /locus_tag="OO130_000121"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HCR5363674.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLVAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGESMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            24228..24586
                     /gene="gutM"
                     /locus_tag="OO130_000122"
                     /pseudo
     CDS             24228..24586
                     /gene="gutM"
                     /locus_tag="OO130_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
     gene            24653..25426
                     /gene="srlR"
                     /locus_tag="OO130_000123"
                     /gene_synonym="gutR"
     CDS             24653..25426
                     /gene="srlR"
                     /locus_tag="OO130_000123"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HCR5363675.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFSFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            25419..26384
                     /gene="gutQ"
                     /locus_tag="OO130_000124"
     CDS             25419..26384
                     /gene="gutQ"
                     /locus_tag="OO130_000124"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HCR5363676.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(26381..27895)
                     /gene="norR"
                     /locus_tag="OO130_000125"
     CDS             complement(26381..27895)
                     /gene="norR"
                     /locus_tag="OO130_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HCR5363677.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKGVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMMPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSAGAR
                     NLLQHYRFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGMKD"
     gene            28082..29521
                     /gene="norV"
                     /locus_tag="OO130_000126"
     CDS             28082..29521
                     /gene="norV"
                     /locus_tag="OO130_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HCR5363678.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNKVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFEELASEAK"
     gene            29518..30651
                     /gene="norW"
                     /locus_tag="OO130_000127"
                     /pseudo
     CDS             29518..30651
                     /gene="norW"
                     /locus_tag="OO130_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417191.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
     gene            complement(30779..33031)
                     /gene="hypF"
                     /locus_tag="OO130_000128"
     CDS             complement(30779..33031)
                     /gene="hypF"
                     /locus_tag="OO130_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HCR5363679.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLREDPETFLVQLHQHCPPLARIDSVEREPYIWSQLPTEFTIRQSAGGAMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPDLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPKYVV
                     HDAHPGYVSSQWAREMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCHGVTHPVTMPLVDNQLDLAAFWQQWLNWQAQVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(33184..33711)
                     /gene="hydN"
                     /locus_tag="OO130_000129"
     CDS             complement(33184..33711)
                     /gene="hydN"
                     /locus_tag="OO130_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HCR5363680.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(33860..34870)
                     /gene="ascG"
                     /locus_tag="OO130_000130"
     CDS             complement(33860..34870)
                     /gene="ascG"
                     /locus_tag="OO130_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HCR5363681.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALAQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAMKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIHRDSLIAL
                     SR"
     gene            35130..36587
                     /gene="ascF"
                     /locus_tag="OO130_000131"
     CDS             35130..36587
                     /gene="ascF"
                     /locus_tag="OO130_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HCR5363682.1"
                     /translation="MAKNYAALARSEIAALGGVDNISAVTHCMTRLRFVIKDDQLIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTPRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYSIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEAAAQARKHQSVQPTVAKEVSLN"
     gene            36596..38020
                     /gene="ascB"
                     /locus_tag="OO130_000132"
     CDS             36596..38020
                     /gene="ascB"
                     /locus_tag="OO130_000132"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HCR5363683.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGD
                     EITPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LAAKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPA
                     YSARVFREKGVTIDKALGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(38179..38649)
                     /gene="hycI"
                     /locus_tag="OO130_000133"
     CDS             complement(38179..38649)
                     /gene="hycI"
                     /locus_tag="OO130_000133"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HCR5363684.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            complement(38642..39052)
                     /gene="hycH"
                     /locus_tag="OO130_000134"
     CDS             complement(38642..39052)
                     /gene="hycH"
                     /locus_tag="OO130_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HCR5363685.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQQEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            complement(39049..39816)
                     /gene="hycG"
                     /locus_tag="OO130_000135"
     CDS             complement(39049..39816)
                     /gene="hycG"
                     /locus_tag="OO130_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HCR5363686.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGELDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDFLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEVRIR"
     gene            complement(39816..40358)
                     /gene="hycF"
                     /locus_tag="OO130_000136"
     CDS             complement(39816..40358)
                     /gene="hycF"
                     /locus_tag="OO130_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HCR5363687.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            complement(40368..42077)
                     /gene="hycE"
                     /locus_tag="OO130_000137"
     CDS             complement(40368..42077)
                     /gene="hycE"
                     /locus_tag="OO130_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HCR5363688.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKEQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(42095..43018)
                     /gene="hycD"
                     /locus_tag="OO130_000138"
     CDS             complement(42095..43018)
                     /gene="hycD"
                     /locus_tag="OO130_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HCR5363689.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTAGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITSRITWAGFGFAFLAFVSLLAA"
     gene            complement(43021..44847)
                     /gene="hycC"
                     /locus_tag="OO130_000139"
     CDS             complement(43021..44847)
                     /gene="hycC"
                     /locus_tag="OO130_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HCR5363690.1"
                     /translation="MSAISLINSGVVWFVAAAVLAFLFSFQKTLSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLAIACWLLWQRYGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGILILSLLGGNAPLWWGIVLLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(44844..45455)
                     /gene="hycB"
                     /locus_tag="OO130_000140"
     CDS             complement(44844..45455)
                     /gene="hycB"
                     /locus_tag="OO130_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HCR5363691.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNVITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARISTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(45580..46041)
                     /gene="hycA"
                     /locus_tag="OO130_000141"
     CDS             complement(45580..46041)
                     /gene="hycA"
                     /locus_tag="OO130_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HCR5363692.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKEGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            46253..46603
                     /gene="hypA"
                     /locus_tag="OO130_000142"
     CDS             46253..46603
                     /gene="hypA"
                     /locus_tag="OO130_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCR5363693.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEVECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            46607..47479
                     /gene="hypB"
                     /locus_tag="OO130_000143"
     CDS             46607..47479
                     /gene="hypB"
                     /locus_tag="OO130_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HCR5363694.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            47470..47742
                     /gene="hypC"
                     /locus_tag="OO130_000144"
     CDS             47470..47742
                     /gene="hypC"
                     /locus_tag="OO130_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HCR5363695.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVITEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            47742..48863
                     /gene="hypD"
                     /locus_tag="OO130_000145"
     CDS             47742..48863
                     /gene="hypD"
                     /locus_tag="OO130_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HCR5363696.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            48860..49870
                     /gene="hypE"
                     /locus_tag="OO130_000146"
     CDS             48860..49870
                     /gene="hypE"
                     /locus_tag="OO130_000146"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HCR5363697.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPCYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISESALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            49944..52022
                     /gene="flhA"
                     /locus_tag="OO130_000147"
                     /pseudo
     CDS             49944..52022
                     /gene="flhA"
                     /locus_tag="OO130_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
     gene            complement(52059..52412)
                     /locus_tag="OO130_000148"
     CDS             complement(52059..52412)
                     /locus_tag="OO130_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HCR5363698.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
     gene            complement(52489..52623)
                     /locus_tag="OO130_000149"
     CDS             complement(52489..52623)
                     /locus_tag="OO130_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5363699.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIASVE
                     "
     gene            52699..55260
                     /gene="mutS"
                     /locus_tag="OO130_000150"
     CDS             52699..55260
                     /gene="mutS"
                     /locus_tag="OO130_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HCR5363700.1"
                     /translation="MSAIENFDAHTPMMQQYLRLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAGLQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     DVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            55366..56022
                     /gene="pphB"
                     /locus_tag="OO130_000151"
     CDS             55366..56022
                     /gene="pphB"
                     /locus_tag="OO130_000151"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HCR5363701.1"
                     /translation="MPSTRYQKINAHHYRHIWVVGDIHGEYQLLQSRLHQLSFCPETD
                     LLISVGDNIDRGPESLDVLRLLNQPWFISVKGNHEAMELDAFATGDGNMWLASGGDWF
                     FDLNDSEQQEAIDLLLKFHHLPHIIEITNDIIKYVIAHADYPGDEYLFGKEIAESELL
                     WPVYRVQKSLNGELQQINGADYFIFGHMMFDNIQTFANQIYIDTGSPKSGRLSFYKIR
                     "
     gene            complement(56073..56840)
                     /locus_tag="OO130_000152"
     CDS             complement(56073..56840)
                     /locus_tag="OO130_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR5363702.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRMNVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            57036..57944
                     /locus_tag="OO130_000153"
     CDS             57036..57944
                     /locus_tag="OO130_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCR5363703.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGLSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGDAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            57941..59203
                     /locus_tag="OO130_000154"
     CDS             57941..59203
                     /locus_tag="OO130_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HCR5363704.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQQGCKQIYFKYCSTFDSTAKGNIGPVTDAL
                     MDALDTPFTVFSPALSVNGRTVYQGYLFVMNQLLAESGMRHHPVNPMTDSYLPRLVEA
                     QSTGRCGVVSAHVFEQGVDAVRQELARLQQEGYRYAVLDALTEHHLEIQGEALRDAPL
                     VTGGSGLAIGLARQWAQENGNQAREAGHPLAGRGVVLSGSCSQMTNRQVAHYRQIAPA
                     REVDVARCLSTETLAAYAHELAEWVLGQESVLAPLVFATASTDALAAIQQQYGAQKAS
                     QAVETLFSQLAARLAAEGVTRFIVAGGETSGVVTQSLGIKGFHIGPTISPGVPWVNAL
                     DKPVSLALKSGNFGDEAFFSRAQREFLS"
     gene            59200..59838
                     /locus_tag="OO130_000155"
     CDS             59200..59838
                     /locus_tag="OO130_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HCR5363705.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVAADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTVLSCLQGLDSSNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAGEIAELRS"
     gene            59843..60618
                     /locus_tag="OO130_000156"
                     /pseudo
     CDS             59843..60618
                     /locus_tag="OO130_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
     gene            60707..62071
                     /locus_tag="OO130_000157"
     CDS             60707..62071
                     /locus_tag="OO130_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HCR5363706.1"
                     /translation="MSTITLLCIALAGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSGGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVSFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVATAGLLHADIGWLTIIGIAISIPVGIVGYFAAKIINKRQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMTGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTSDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALRASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCV
                     WAVI"
     gene            complement(62165..63157)
                     /gene="rpoS"
                     /locus_tag="OO130_000158"
     CDS             complement(62165..63157)
                     /gene="rpoS"
                     /locus_tag="OO130_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HCR5363707.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(63220..64311)
                     /gene="nlpD"
                     /locus_tag="OO130_000159"
     CDS             complement(63220..64311)
                     /gene="nlpD"
                     /locus_tag="OO130_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HCR5363708.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKMGTTSTAQQPQIQPVQPVAQQPVQMENGRIVYNRQYGNIPKGSY
                     SGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVGQTLQVGNASGTPITGG
                     NAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSANKMLPNNKPAATTVTA
                     PVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASEGGNKGIDIAGSKGQAI
                     IATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLVREQQEVKAGQKIATMG
                     STGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(64451..65077)
                     /gene="pcm"
                     /locus_tag="OO130_000160"
     CDS             complement(64451..65077)
                     /gene="pcm"
                     /locus_tag="OO130_000160"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HCR5363709.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(65071..65832)
                     /gene="surE"
                     /locus_tag="OO130_000161"
     CDS             complement(65071..65832)
                     /gene="surE"
                     /locus_tag="OO130_000161"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HCR5363710.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(65813..66862)
                     /gene="truD"
                     /locus_tag="OO130_000162"
     CDS             complement(65813..66862)
                     /gene="truD"
                     /locus_tag="OO130_000162"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HCR5363711.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQHRVNDKELMITAALPGSGEWGTQRKALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(66859..67338)
                     /gene="ispF"
                     /locus_tag="OO130_000163"
     CDS             complement(66859..67338)
                     /gene="ispF"
                     /locus_tag="OO130_000163"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HCR5363712.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(67338..68048)
                     /gene="ispD"
                     /locus_tag="OO130_000164"
     CDS             complement(67338..68048)
                     /gene="ispD"
                     /locus_tag="OO130_000164"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HCR5363713.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(68067..68378)
                     /gene="ftsB"
                     /locus_tag="OO130_000165"
     CDS             complement(68067..68378)
                     /gene="ftsB"
                     /locus_tag="OO130_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HCR5363714.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(68572..68895)
                     /locus_tag="OO130_000166"
     CDS             complement(68572..68895)
                     /locus_tag="OO130_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HCR5363715.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            complement(68945..69550)
                     /gene="cysC"
                     /locus_tag="OO130_000167"
     CDS             complement(68945..69550)
                     /gene="cysC"
                     /locus_tag="OO130_000167"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HCR5363716.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(69550..70977)
                     /gene="cysN"
                     /locus_tag="OO130_000168"
     CDS             complement(69550..70977)
                     /gene="cysN"
                     /locus_tag="OO130_000168"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HCR5363717.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTHIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMSWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKALPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     PSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYPVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPKNEAAVASSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(70979..71887)
                     /gene="cysD"
                     /locus_tag="OO130_000169"
     CDS             complement(70979..71887)
                     /gene="cysD"
                     /locus_tag="OO130_000169"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HCR5363718.1"
                     /translation="MAQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            72139..73176
                     /gene="iap"
                     /locus_tag="OO130_000170"
     CDS             72139..73176
                     /gene="iap"
                     /locus_tag="OO130_000170"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HCR5363719.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKSWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTTEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTAAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            complement(73383..73676)
                     /gene="cas2"
                     /locus_tag="OO130_000171"
     CDS             complement(73383..73676)
                     /gene="cas2"
                     /locus_tag="OO130_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2"
                     /protein_id="HCR5363720.1"
                     /translation="MSMVVVVTENVPPRLRGRLAIWLLEVRAGVYVGDTSKRIREMIW
                     QQITQLAGCGNVVMAWATNTESGFEFQTWGENRRIPVDLDGLRLVSFLPVDNQ"
     gene            complement(73676..74596)
                     /gene="cas1e"
                     /locus_tag="OO130_000172"
     CDS             complement(73676..74596)
                     /gene="cas1e"
                     /locus_tag="OO130_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1"
                     /protein_id="HCR5363721.1"
                     /translation="MTFVPLSPIPLKDRTSMIFLQYGQIDVLDGAFVLIDKTGIRTHI
                     PVGSVACIMLEPGTRVSHAAVHLAATVGTLQVWVGEAGVRVYSSGQPGGARADKLLYQ
                     AKLALTEDLRLKVVRKMYELRFREPPPARRSVEQLRGIEGSRVRQTYALLAKQYGVKW
                     NGRKYDPKDWEKGDVVNRCISAATSCLYGISEAAVLAAGYAPAIGFIHSGKPLSFVYD
                     IADIIKFDSVVPKAFEIAARQPAEPDKEVRLACRDIFRSTKLTGKLIPLIEEVLAAGE
                     IEPPQPAPDMLPPAIPEPETLGDSGHRGRG"
     gene            complement(74593..75243)
                     /gene="cas6e"
                     /locus_tag="OO130_000173"
     CDS             complement(74593..75243)
                     /gene="cas6e"
                     /locus_tag="OO130_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HCR5363722.1"
                     /translation="MYLSRITLHTGQLSPAQLLHLVDRGEYVMHQWLWDLFPSGKERQ
                     FLYRREELQGAFRFFVLSQERPAESDTFTIECRSFAPELCTGQQLCFNLRANPTICKA
                     GKRHDLLMEAKRQVRGQAEGSDVWLHQQQAALDWLAAQGERSGFTLLDISVDAYRQQQ
                     LRRENSRQLIQFSSVDYTGMLTVTDPGLFLQRLSQGYGKSRAFGCGLMLIKPGAEA"
     gene            complement(75225..75971)
                     /gene="cas5e"
                     /locus_tag="OO130_000174"
     CDS             complement(75225..75971)
                     /gene="cas5e"
                     /locus_tag="OO130_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HCR5363723.1"
                     /translation="MSQYLIFQLHGPMASWGVDAPGEVRHTHELPSLSALLGLLAAGV
                     GIRRDDTERLNAFNRHYSLVVCASRNPRWARDYHTVQMPKEVRKARYFSRREELSDPE
                     LLSAIISRRDYYTDAWWMVAVATTADAPYSLEQLQDGLRHPVFPLYLGRKSHPLALPL
                     APLLLEGNACDALCNAYQQYQDHFHKLKVSLPKLQDECWWEGKHDGLVASKILRRRDV
                     PLNRQQWLFGERTVNQGPWLSKEEPCTSQE"
     gene            complement(75982..77060)
                     /gene="cas7e"
                     /locus_tag="OO130_000175"
                     /pseudo
     CDS             complement(75982..77060)
                     /gene="cas7e"
                     /locus_tag="OO130_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311639.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
     gene            complement(77075..77622)
                     /gene="casB"
                     /locus_tag="OO130_000176"
                     /pseudo
     CDS             complement(77075..77622)
                     /gene="casB"
                     /locus_tag="OO130_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311640.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
     gene            complement(77619..79181)
                     /gene="casA"
                     /locus_tag="OO130_000177"
     CDS             complement(77619..79181)
                     /gene="casA"
                     /locus_tag="OO130_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
                     /protein_id="HCR5363724.1"
                     /translation="MNSFSLLTTPWLPVRFKDGTTGKLAPVDLADENVVDIAAPRADL
                     QGAAWQFLLGLLQTSFAPKDQRSWDDIWEDGLEAEKLREALLSLEHAFQFGPDSPSFM
                     QDFDELKVKATFIASLLPDAPGKQTKERNTDHFIKRDTTQHLCLHCVPLALFSIQLNA
                     PIGGRGYYPGLRGGGPLTTLIELLEYQGNQQTPLWRKLWLNVMPQDEADLPLPKTFDD
                     LVFPWLAPTRTSELDGAVVTDEQVNKLQAYWGMPRRIRIDFKTTSIGNCDICGRQSDA
                     LLGLMSLKNYGVQYVMWRHPLTPYRLPLKEGGDFYSVKPQPGGLIWRDWLGLIEVGNS
                     KNNTELPAQVVKLLNASNLKQTRVGLWGFGFDFEDMKVRCWYEHHFPLLLNKKEDLIP
                     KLRLAAQAASHILSLLHRALKEAWFSEKKTTKLDFGFVDIDFWNKTQHRFLRLVRKIE
                     EGQDPDELLSKWQKEMWLFARQDFDDRVFTNPYEPVDLERVMTARKKYFTTSAEKQNA
                     NAAREKKQEAAE"
     gene            complement(79279..80833)
                     /gene="cas3"
                     /locus_tag="OO130_000178"
                     /pseudo
     CDS             complement(79279..80833)
                     /gene="cas3"
                     /locus_tag="OO130_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000142534.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
BASE COUNT        19061 a        21958 c        20657 g        19157 t
ORIGIN      
        1 gctcctctta tgtgatgggg aaagcggttg aaacagaaga tgaagactgg aatttctgag
       61 ggtgttattt tcaaaaatat cactacccgc agcagggaaa taattcccgc caaatagctt
      121 tttatcacgc aaatattctg tagtgatcta cactgagact ctgttgcatt attcgcctga
      181 aactacaata ttcaggcgtt ttttcgctat ctatgacaaa aaatatcaac ttttctcgat
      241 ttgctctcag cccttatatc acgggaaatt ccggcgattt gctcgcatca atattcatgc
      301 cacatttgcc ctcaggggtt gcctcagatt ctcagtatgt tagggtagaa aaaagtgact
      361 atttccattg ggtaatatat cgacataaac aaataaagga atctttctat tgcatggcaa
      421 ttaaattaga aattaaaaat ctttataaaa tatttggcga gcatccacag cgagcgttca
      481 aatatatcga acaaggactt tcgaaagaac aaattctgga aaaaactggg ctatcgcttg
      541 gcgtaaaaga cgccagtctg gccattgaag aaggcgagat atttgtcatc atgggattat
      601 ccggctcggg taaatccaca atggtacgcc ttctcaatcg cctgattgaa cccacccgcg
      661 ggcaagtgct gattgatggt gtggatattg ccaaaatatc cgatgccgaa ctccgtgagg
      721 tgcgcagaaa aaagattgcg atggtcttcc agtcctttgc cttaatgccg catatgaccg
      781 tgctggacaa tactgcgttc ggtatggaat tggccggaat taatgccgaa gaacgccggg
      841 aaaaagccct tgatgcactg cgtcaggtcg ggctggaaaa ttatgcccac agctacccgg
      901 atgaactctc tggcgggatg cgtcaacgtg tgggattagc ccgcgcgtta gcgattaatc
      961 cggatatatt attaatggac gaagccttct cggcgctcga tccattaatt cgcaccgaga
     1021 tgcaggatga gctggtaaaa ttacaggcga aacatcagcg caccattgtc tttatttccc
     1081 acgatctcga tgaagctatg cgtattggcg accgaattgc cattatgcaa aatggtgaag
     1141 tggtacaggt cggcacaccg gatgaaattc tcaataatcc ggcgaatgat tatgtccgta
     1201 ccttcttccg tggcgttgat attagtcagg tattcagtgc gaaagatatt gcccgccgga
     1261 caccgaatgg cttaattcgt aaaacccctg gcttcggccc acgttcggca ctgaaattat
     1321 tgcaggatga agatcgcgaa tatggctacg ttatcgaacg cggtaataag tttgtcggcg
     1381 cagtttccat cgattcgctt aaaaccgcgt taacgcagca gcaaggtctt gatgcggcgc
     1441 tgattgatgc gccgttagca gtcgatgcac aaacgcctct tagcgagttg ctctctcatg
     1501 tcggacaggc accctgtgcg gtacccgtgg tcgacgagga ccaacagtat gtcggcatca
     1561 tttcgaaagg aatgctgctg cgcgctttag atcgtgaggg ggtaaataat ggctgatcaa
     1621 aataatccgt gggataccac gccagcggcg gacagtgccg tgcaatccgc agacgcctgg
     1681 ggtacaccgg cgactgcacc gactgacggc ggtggtgctg actggctgac cagtacgcct
     1741 gcgccaaacg tcgagcattt taatattctc gatccgttcc ataaaacgct gatcccgctc
     1801 gacagttggg tcactgaagg gatcgactgg gttgttaccc atttccgtcc cgtcttccag
     1861 ggcgtgcgcg ttccggttga ttatatcctc aacggtttcc agcaagtgct gctgggtatg
     1921 cccgcaccgg tggcgattat cgttttcgct ctcatcgcct ggcagatttc cggggtcgga
     1981 atgggcgtgg cgacgctggt ttcgctgatt gccatcggcg caatcggtgc ctggtcgcag
     2041 gccatggtta ccctggcgct ggtgttaacc gccctgctgt tctgtatcgt cattggtttg
     2101 ccgctgggga tctggctggc gagaagtccg cgagcggcga aaattattcg tccactgctt
     2161 gatgccatgc agaccacgcc agcgtttgtt tatctggtgc caatcgtcat gctatttggt
     2221 atcggtaacg tgccgggcgt ggtggtgacg atcatctttg ctctgccgcc gattatccgt
     2281 ctgaccattc tggggattaa ccaggttccg gcggatctga ttgaagcctc gcgctcattc
     2341 ggtgccagcc cgcgccagat gctgttcaaa gttcagttac cgctggcgat gccgagcatt
     2401 atggcgggcg ttaaccagac gctgatgctg gccctttcta tggtggtcat tgcctcgatg
     2461 attgccgtcg gcgggctggg tcagatggta cttcgcggta tcggtcgtct ggatatgggg
     2521 cttgccaccg ttggcggcgt cgggattgtg atcctcgcca ttatcctcga tcgcctgacg
     2581 caggccgttg ggcgcgactc acgcagtcgc ggcaaccgtc gctggtacac cactggccct
     2641 gttggtctgc tgacccgccc attcattaag taactctgca cttgcccggt gacgccgggc
     2701 attatcaccc tgccaaaaaa aggaataaca atgcgacata gcgtactttt tgcgacagcg
     2761 tttgccacgc ttatctctac acaaactttt gctgccgatc tgccgggcaa aggtattact
     2821 gttaatccag ttcaaagcac catcaccgaa gaaaccttcc agacgctgct ggtcagccgt
     2881 gcgctggaga aattaggcta taccgtcaac aaacccagcg aagtagatta caacgttggc
     2941 tacaccttgc ttgcttccgg cgatgcaacc ttcaccgccg tgaactggac gccactgcat
     3001 gacaacatgt acgaagctgc cggtggcgat aagaaatttt atcgtgaagg ggtatttgtt
     3061 aacggcgcgg cacagggtta cctgatcgat aagaaaaccg ccgaccagta caaaatcacc
     3121 aacatcgcac aactgaaaga gccgaagatc gccaaactgt tcgataccaa cggcgacggt
     3181 aaagcggatt taaccggttg taatcctggc tggggctgcg aaggtgcgat caaccaccag
     3241 cttgccgcgt atgaactgac caacaccgtg acgcataatc aggggaacta cgcagcgatg
     3301 atggccgaca ccatcagtcg ctacaaagag ggcaaaccgg tgttttatta cacctggacg
     3361 ccgtactggg tgagtaatga gctgaagcca gggaaagatg tggtctggtt gcaggtgccg
     3421 ttctccgcac tgccgggcga taaaaatgcc gataccaaac tgccgaatgg cgcgaattac
     3481 ggcttcccgg tcagcaccat gcatatcgtt gccaacaaag cctggaccga gaaaaatccg
     3541 gcggcagcca aactgtttgc cattatgcag ttgccagtgg ccgatattaa cgcccagaac
     3601 gccattatgc atgacggcaa agcctcagaa ggcgatattc agggccacgt tgatggctgg
     3661 atcaaagccc accagcagca gttcgatggc tgggtgaatg aggcgctggc agcgctgaag
     3721 taatttttat tcaggcggat aaggcgtttt tgccttatcc gccgctctgt acacaatgcc
     3781 tgatgcgacg ctgacgcgtc ttatcatgcc tacaaaatac actcattccg tatggcggat
     3841 aaggcgtttt cgccgcatcc gccgttctgt gcacaatgcc tgatgcgacg ctggcgcgtc
     3901 ttatcatgcc tacaaaccac atcgcacatt tcaacaagct atctttcatc ccatattcat
     3961 caacatccgc tattattgat ttccagctta atcatcacct gatgaacaaa aataatgact
     4021 aaacctaatc atgagcttag cccggcgctg atcgtgctga tgtctatcgc caccggtctg
     4081 gcggtagcca gtaactatta cgcccagcca ttgctcgaca ccatcgcgcg taacttttcc
     4141 ctttccgcca gttcggcagg ctttattgtt accgccgcgc aattgggcta tgcagcaggt
     4201 ctgctgtttc ttgttcccct cggtgatatg tttgaacgcc gccgcctgat tgtctcgatg
     4261 accttactgg cggcaggcgg catgttaatc actgccagca gtcagtcgct ggcgatgatg
     4321 atcctcggta cggcattaac cggtttattc tctgtcgtgg cacaaattct ggttccgctg
     4381 gcagcgacgc tggcttcacc ggacaaacgc ggcaaagtgg ttggcactat tatgagcggt
     4441 ctgctgttgg ggatcttact cgcacggacc gttgccggat tgctggcgaa tctcggcggc
     4501 tggcgcaccg tcttttgggt tgcctcggtg ttaatggcac tgatggcgct ggcattatgg
     4561 cgtggtctgc cacaaatgaa atcagaaacc cacctcaact acccacagtt gttaggttcc
     4621 gttttcagca tgtttatcag cgataagatc ctgcgccccc gcgcattgct gggctgcctg
     4681 acctttgcca acttcagcat tctctggacc tcaatggcct ttttgcttgc cgctccacct
     4741 tttaactaca gcgatggtgt cattggtctg tttggacttg cgggagctgc cggagcgttg
     4801 ggcgctcgtc cggcgggcgg ttttgccgat aagggtaaat cgcaccacac cacaactttc
     4861 ggtctgctgc tgctattact ttcatggctg gcgatctggt ttggtcacac ttccgtactg
     4921 gcgctgatta tcggcatcct ggtgctggat ctcaccgtac agggcgtgca tatcactaac
     4981 cagacggtaa tttatcgaat acaccctgat gcgcgtaatc gcctgaccgc aggttacatg
     5041 accagctact ttattggcgg tgccgccggt tcgctaattt cagcctcagc ctggcaacat
     5101 ggcggttggg ctggcgtttg tctggctggc gcgacgattg ccctggttaa cttactggtc
     5161 tggtggcgag gttttcatcg tcaggaagcc gcaaattaag caatcagcaa ccgttttcgt
     5221 ggctttacac ttataagggt gttaagaagc ccatcagtct gataaggtta agatattcat
     5281 tcagtctatt tataatatta acaatcgtta agcgtacact ctatggaaag ccctactcca
     5341 cagcctgctc ttggttcggc gaccttcatg gaaggatgca aagacagttt accgattgtt
     5401 attagttata ttccggtggc ctttgcgttc ggtctgaatg cgacccgtct gggattctct
     5461 cctctcgaaa gcgttttttt ctcctgcatc atttatgcag gcgcgagcca gttcgtcatt
     5521 accgcgatgc tggcagccgg gagtagtttg tgggttgctg cactgaccgt catggcaatg
     5581 gatgttcgcc atgtgttgta tggcccgtca ctgcgtagcc gtattattca gcgtctgcaa
     5641 aaatcgaaaa ccgccctgtg ggcatttggc ctgacggatg aggtttttgc cgccgcaacc
     5701 gcaaaactgg tacgcaataa tcgccgctgg agcgagaact ggatgatcgg cattgccttc
     5761 agttcatggt catcgtgggt atttggtacg gtaatagggg cattctccgg cagcggcttg
     5821 ctgcaaggtt atcccgccgt tgaagctgca ttaggtttta tgcttccggc actctttatg
     5881 agtttcctgc tcgcctcttt ccagcgcaaa caatctcttt gcgttaccgc agcgttagtt
     5941 ggtgcccttg caggcgtaac gctattttct attcccgtcg ccattctggc aggcattgtc
     6001 tgtggctgcc tcactgcgtt aatccaggca ttctggcaag gagcgcccga tgagctatga
     6061 ggttctgctg cttgggttac tggttggcgt ggcgaattat tgcttccgct atttgccgct
     6121 gcgcctgcgt gtgggtaatg cccgcccaac caaacgtggc gcggtaggta ttttgctcga
     6181 caccattggc atcgcctcga tatgcgctct gctggttgtc tctaccgcac cagaagtgat
     6241 gcacgataca cgccgtttcg tgcccacgct ggtcggcttc gcggtactgg gtgccagttt
     6301 ctataaaaca cgcagcatta tcatcccaac actgcttagt gcgctggcct atgggctcgc
     6361 ctggaaagtg atggcgatta tataacccac aagaatcatt tttctaaaac aatacattta
     6421 ctttatttgt tactgtcgtt actatatcgg ctgaaattaa tgaggtcata cccaaatgga
     6481 tagttcgttt acgcccattg aacaaatgct aaaatttcgc gccagccgcc acgaagattt
     6541 tccttatcag gagatccttc tgactcgtct ttgcatgcac atgcaaagca agctgctgga
     6601 gaaccgcaat aaaatgctga aggctcaggg gattaacgag acgttgttta tggcgttgat
     6661 tacgctggag tctcaggaaa accacagtat tcagccttct gaattaagtt gtgctcttgg
     6721 atcatcccgt accaacgcga cgcgtattgc cgatgaactg gaaaaacgcg gttggatcga
     6781 acgtcgtgaa agcgataacg atcgccgctg cctgcatctg caattaacgg aaaaaggtca
     6841 cgagtttttg cgcgaggttt taccaccgca gcataactgc ctgcatcaac tctggtccgc
     6901 gctcagcaca acagaaaaag atcagctcga gcaaatcacc cgcaaattgc tctcccgtct
     6961 cgaccagatg gaacaagacg gtgtggttct cgaagcgatg agctaacgcg tcatctcgct
     7021 caaaaatcca gatttataaa agaaaaaatg accggccagc atcgcaacat gctggccttt
     7081 ttggcaagca ggtcggctca gccgatgagt taagaagatc gtggagaaca atatgagcgc
     7141 aaatgcggag actcaaaccc cgcagcaacc ggtaaagaag agcggcaaac gtaagcgtct
     7201 gctcctcctt ctcaccttgc tctttataat tattgccgta gcgataggga tttattggtt
     7261 tttggtactg cgtcatttcg aagaaaccga tgacgcatac gtggcaggga atcagattca
     7321 aattatgtct caggtgtctg gcagcgtgac gaaagtctgg gccgataaca ccgattttgt
     7381 aaaagaaggc gatgttctgg tcactctcga cccgacagat gctcgccagg cgtttgaaaa
     7441 agccaaaact gcactggctt ccagcgttcg ccaaacccac cagctgatga ttaacagcaa
     7501 acagttacag gcgaatattg aggtgcaaaa aatcgccctc gcgaaagcac aaagcgacta
     7561 caaccgccgt gtgccgctgg gcaatgccaa cctgattggt cgcgaagagc tgcaacacgc
     7621 ccgcgacgcc gtcaccagtg cccaggcgca actggacgtc gctattcaac aatacaatgc
     7681 caatcaggcg atgattctgg ggactaaact ggaagatcag cctgccgtgc aacaggctgc
     7741 caccgaagta cgtaacgcct ggctggcgct ggagcgtacc cgtattgtca gtccgatgac
     7801 cggttatgtc tcccgccgcg cggtacagcc tggggcgcaa attagcccaa cgacgccgct
     7861 gatggcggtc gttccagcca ccaatatgtg ggtggatgcc aactttaaag agacgcagat
     7921 tgccaatatg cgtatcggtc agccggtcac tatcaccacg gatatttacg gcgatgatgt
     7981 gaaatacacc ggtaaagtgg ttggtctgga tatgggcaca ggtagcgcat tctcactgct
     8041 tccggcgcaa aatgcgaccg gtaactggat caaagtcgtt cagcgtctac ctgtgcgtat
     8101 cgaactggac cagaaacagc tggagcaata cccgctgcgc attggtttgt ccacgctggt
     8161 gagcgtcaat accactaacc gtgacggtca ggtactggca aataaagtac gttcaacacc
     8221 ggtcgcggta agtaccgtgc gtgaaatcag cctggcacct gtcaataaac tgatcgacga
     8281 tatcgtaaaa gctaacgctg gctaatccag aggtgcgtgt gatgcaacag caaaaaccgc
     8341 tggaaggcgc gcaactggtc attatgacga ttgcgctgtc actggcgaca ttcatgcagg
     8401 tgctggactc caccattgct aacgtggcga tccccactat cgccgggaat ctgggctcat
     8461 cgctcagcca gggaacgtgg gtaatcactt ctttcggggt ggcgaatgcc atctcgatcc
     8521 cgcttaccgg ctggctggca aagcgcgtcg gggaagtgaa actgttcctt tggtccacca
     8581 tcgcctttgc tattgcgtcg tgggagtgtg gtgtctccag cagcctgaat atgctgatct
     8641 tcttccgtgt gattcagggg attgtcgctg ggccgttgat cccactttcg caaagtcttt
     8701 tgctgaataa ttacccgcca gccaaacgct caatcgcgct ggcgttgtgg tcgatgacgg
     8761 tgattgtcgc gccaatttgc ggcccaatcc tcggcggtta tatcagcgat aattaccact
     8821 ggggctggat attcttcatc aacgtgccaa ttggcgtggc ggtggtgttg atgacactgc
     8881 aaaccctgcg cggacgcgaa acccgcaccg aacggcggcg gattgatgcc gtggggctgg
     8941 cactgctggt tattggtatc ggtagcctgc agattatgct cgaccgcggt aaagagctgg
     9001 actggttttc atcacaggaa attatcattc ttaccgtggt ggcggtggtg gctatctgct
     9061 tcctgattgt ctgggagctg accgacgata acccgatagt cgatctgtcg ttgtttaagt
     9121 cgcgcaactt caccatcggc tgcttgtgta tcagcctcgc gtatatgctc tacttcggcg
     9181 cgattgttct gctgccgcag ttgttgcagg aggtctacgg ttacacggcg acctgggcag
     9241 gtttggcctc tgcgccggta gggattattc cggtgatcct gtcgccgatt atcggccgct
     9301 tcgcgcataa actggatatg cggcggctgg taaccttcag ctttattatg tatgccgtct
     9361 gcttctactg gcgtgcctat acctttgaac caggtatgga ttttggcgcg tcggcctggc
     9421 cgcagtttat ccaggggttt gcggtggcct gcttctttat gccgctgacc accattacgc
     9481 tgtctggttt gccaccggaa cgactggcgg cggcatcgag cctctctaac tttacgcgaa
     9541 cgctggcggg gtctatcggc acgtcgataa ccacgaccat gtggaccaac cgcgagtcga
     9601 tgcaccatgc gcagttgact gagtcggtaa acccgttcaa cccgaatgcc caggcgatgt
     9661 acagtcaact ggaagggctt gggatgacgc aacagcaggc atcaggctgg attgcccagc
     9721 agatcactaa tcaggggctg attatttccg ccaatgagat cttctggatg tcagccggga
     9781 tattcctcgt cctgctgggg ctggtgtggt ttgctaaacc gccctttggc gcaggtggcg
     9841 gcggaggtgg tgcgcactaa gtacaactaa gccagttcat ttgaactggc ttttttcaat
     9901 taattgtgaa gatagtttac tgactaaatg tgcagttcct gcaacttctc tttcggcagt
     9961 gccagttctt cgttgctgtt gatgcgtacg tcacgttcca gaatgctacg cgcaatatcc
    10021 tgcgcttcct gcaacgagtg catctggtaa gtgccacact ggtagacgtt cagctccggg
    10081 atctgattct gatcctgcac tttcagcacg tcttccattg ccgctttcca ggcatcagca
    10141 acacgctgct catctggcgt accaatcaga ctcatataaa aaccggtgcg gcagcccatt
    10201 ggcgagatat cgataatctc tacaccatta ccgttaagat ggttacgcat aaaaccagca
    10261 aacaggtgct ccagagtatg gctccctctt tctggcatca cttctttgtt cggcacgcag
    10321 aagcgcagat cgaacacggt gattgcgtcg ccatgcgggg tgttcattgt tttcgccacc
    10381 cgaactgcag gcgcttccat ccgggtatga tcgactgtga agctatctaa caacggcatt
    10441 tagccacctc cggtaatttt tttaaaaatt ttctgaactc tttcttccca ggcgagtctg
    10501 agtatatgaa agacgcgcat ttgttatcat catccctgaa ttcagagatg aaattttggc
    10561 cactcacgag tggccttttt cttttctgtc aggcgtgttt ttccagccac accgcaaacg
    10621 gttcggtatc agcggtttcc atttcctgct gacggcgttc agatgcctcg cgctcggcta
    10681 caaaatcctc ttcgcgcaga atttccaacg gctcttcacg cagcagatta cggtaggctt
    10741 ctgcaaacgc tttgcctgtt ccgccaatac cagtatcaat catagacctt aagatacggg
    10801 cagagaaagt cagatcggga ttatcgaagc aggcgaccag ttcatcacac actttctgat
    10861 acgctttgcc gccgttaatg ctatccaacg tttgcgcgac gcgtttcaga tcgcggaaca
    10921 gatctttacc cacctgcggt aacgggaact gcgcggtttc gcagccgata cccagcgtca
    10981 ggcccggttt gcgaccttcg aggatcaccc ggttccagtt aacgcgtgta caggcaagtt
    11041 cgctactgct catttccggt gcatcagcca gcgcacacca gaccataaac aggtcgagga
    11101 atcgcacctg ctgttcatct acaccaatcg gcgagaacgg gttgatgtcc agcgaacgca
    11161 cttcaatata ttcaatgccg ccacgtaaca gcgcatcaga aggtgactcg ccgctgcggg
    11221 taacgcgttt tggacgaatc ggcgcataca gttcgttttc aatctgcagc acgttgctat
    11281 tgatttgcag cctcttaccg tctttctcaa taccaatctt cgcgtactct tccgatggcg
    11341 ttttgattgc ctgtttaagg cccgctacgt actcgtaaag atcgttgaag gtaataccaa
    11401 gattgctttg cgatttattg gtatagccga gatcgctcaa acgaagagag gtcgcatacg
    11461 gcaggtaata cataccgcac tcggttttct caaacggcag cgacgttggt tttccttgca
    11521 ggaaagaaga acaaatcgcc ggagatgcac caaacagata aggaatgacc caaccgaaac
    11581 gatagtaatt gcggataacg cggaaatagc ccgcagaaat tttctctttg gcatcagtgc
    11641 ccgagatatc accgcacttc gcttgccaga atgccattgg caaagagaaa ttgtagtgca
    11701 cgccggaaat ggtttgcatc agcgcgccgt agcgattttt cagcccttca cgatacagcg
    11761 ttttaaagcg tccggtgtta gaagtgccgt actgtgccag ttcgatgtcc tgaccttctg
    11821 cgatgtagca tggcatactt aacggccaca tccgctcatc gcccatattg cgcgccgtat
    11881 aacgatgcag atcgcgcata aaggtcaaca tatgttcaat atcaccatcc actggtgtaa
    11941 tgaattccag caatgcttcc gcaaaatcgg tagtaatcca tttgtgcgtc agtgcggaac
    12001 ctaatgcttc aggatgacct gttgttgcct gtgtgccatc agcattaaca cgcaaagttt
    12061 cgcgctccag cccacgctgt atccccttta acgcctgagg atgtttttcc agccaggcca
    12121 gcgcctgtga tacgtccggg atcaaattga cctcccgcct gtcaaaatcg ttttaattag
    12181 cataactgta atggtgacca tatgtgcagg cctacaatta gtgccaccac atcatgccct
    12241 gaacggtagc tgctgcaact gcaacatagc gtaaagcttt accaaggcac aaaaaaaaga
    12301 ttaccggtcc ccacgagatg cgcatccatc ccgctaacag acacagtaaa tcgccaacca
    12361 cgggcatcca gcttaataat agcgtgactg caccatagcg tttcagccag ccagtagctt
    12421 tctcttgcca gcgcgatgtt ttacgcaatg gaaagaaacg cccaaggata acgttagtta
    12481 accctccaag gctattaccc attgttgctg ttaaaactaa aacccaggga taactgatcc
    12541 cggaaagcaa cattgccacc agcacgactt cggagttgcc gggtaatagt gtagcgctga
    12601 gaaaactact ggcaaacaac gagaaaagcg ataacgcttc actcacagca agcgaacatc
    12661 cacggcgtcc atgcctgctg cacgggccgc ctgaataccg aaatcggcat cttcaaagac
    12721 cacacactgc gtcggttgca cgcccatacg ctgcgcgcac aacaaaaatg tgtctggcgc
    12781 gggtttatgg tgtttgacgt gatcagcagc gacgacggcg tcaaaataac ggcgtaatcc
    12841 caggtgcgcc agcaatgcct cagcgatggc gctttcactc cccgttccta cagccattgg
    12901 gcgacgacca tgccaacttt tcaccacttc aacaagagga agcggctcga cactatccag
    12961 cagcatactt cttactgctt ctgttttttc acgcgctaac gcatgcgggg cgagatcggc
    13021 ctgattcagc tcaataattg cctgagcaat acgccaggtg ggcgatccat taagcgcaat
    13081 cattgcctga acatcgtact gaagaccgta gtgccctaat acttcgcgcc acgctttacg
    13141 gtgcgtaggc tccgtatcca ggattgtgcc atccatatca aaaattaaac ctgcataacg
    13201 ctcgtacatg gtcttctcgc aagtcgaaaa atatgacgct actttagcgt aattgcttga
    13261 ttttgtcgct gatggcgaag gagaagaaaa ttcaggaaat ggataaagta gttatttaga
    13321 aatgagatat ttttgaagga aatttttggg agaagatggt gcatccggga ggattcgaac
    13381 ctccgaccgc tcggttcgta gccgagtact ctatccagct gagctacgga tgcatcggga
    13441 aaatttattt tactgctgat attgatatcg ctactaaagc catatcaagt aagagatggt
    13501 gcatccggga ggattactcg ccttgcggct cgcccttcgg gccgttgcta aagcaacgtt
    13561 atcatcccta gtgcctgcgg ttaattccgc tacctcttcg ataccttcat tgctgaagat
    13621 ttcggagaat atggtgcatc cgggaggatt cgaacctccg accgctcggt tcgtagccga
    13681 gtactctatc cagctgagct acggatgcat cgggaaactt actttactgc agattttttg
    13741 ataccgctac taaagccgta tcaagtaaga gatggtgcat tcgggaggat tactcgcctt
    13801 gcggctcgcc cttctggccg ttgctaaagc aacgttatcc tccctggtgc ctgcggttat
    13861 accgccacct cttccatacc ttcatcgctg aaagtacgta gaatatggtg catccgggag
    13921 gattcgaacc tccgaccgct cggttcgtag ccgagtactc tatccagctg agctacggat
    13981 gcaaatggcg gtgaggcggg gattcgaacc ccggatgcag cttttgaccg catactccct
    14041 tagcagggga gcgccttcag cctctcggcc acctcaccac acgcctctta cgagtgcttc
    14101 gaagaacttg tttatcgctc atcgtcgctg cgtggcgcac atattacttt ctgagactta
    14161 taagtcaaac aatttttccc acacttttat cgtttgcaca cttcacgctc aattagtctg
    14221 taaaaacgtc aaaaagagtg ttttatctac agaaaaacgg aggtctggca gatagcagta
    14281 atgcaaaaaa atggagactt aagttgaatg aacgggagta aagcgaaaag actatagagt
    14341 gaaggagaaa ttttgagggt gcgtctcacc gataaagatg agacgcggaa agattagtaa
    14401 ctggactgct gggatttttc agcctggata cgctggtaga tctcttcacg gtgaacagaa
    14461 acttccttcg gggcatttac gccaatacgt acctggttgc ccttcacccc taaaactgtc
    14521 acggtgacct catccccaat catgagggtc tcaccaactc gacgagtcag aatcagcatt
    14581 ctttgctcct tgaaagatta aaagagtcgg gtctctctgt atcccggcat tatccatcat
    14641 ataacgccaa aaagtaagca atgacaaaca cattacatgt aagcagtcat ggcattacat
    14701 tctgttaaac ctaagtttag ccgatataca caacttcaac ctgactttat cgttgtcgat
    14761 agcgttaatg cgaatgccgt gaagcgagtc cacggcattg cctgacgctt atattattgc
    14821 aatttcgcgc tgacccagcc tttcacactg gctaacgctg caggtaaggc cgcagcatcc
    14881 gtaccaccgg cttgcgccat gtcaggacgt ccaccaccct tgccgcccac ctgctgagcg
    14941 accataccaa tcagttcccc tgctttcaca cgatctgtga cgtccttaga tacgcctgca
    15001 atcagagaaa ccttaccttc ggctaccgtt gccagcacga taattgtcga ccccagctga
    15061 ttttttaaat cgtcaaccat ggtacgcaac attttcggct caacaccgct aagctcgcta
    15121 accaacagct taacaccatt aacatcaatt gccttactgg aaagatttgc gctctcctgt
    15181 gcggcagctt gttctttaag ctgttgtagt tctttttcta actgacgcgt acgttccagt
    15241 actgagcgca ctttatccgc cagattattg ctatcgcctt tcagcagatg cgcgacttcg
    15301 cttaagcgat cgctgtctgc atgaacggtg gtgatagcgc cttctccggt taccgcttcg
    15361 atacgacgaa cgcctgcagc agtacccgat tcagagatga tgcggaacag accaatatca
    15421 ccagtgcggc tggcgtgagt accgccacac agctcggtgg agaaatcgcc catgctcagc
    15481 acgcgtacac gctcatcata cttctcgccg aacagcgcca tcgcaccttt cgctttcgcc
    15541 acttcgagat ccatgatgtt ggtttcgatc ggcaaattgc gacgaatctg tgcgttcacc
    15601 aggtcttcga ccgcacgaat ctcttccggt ttcatcgctt cgttgtgtga gaagtcgaag
    15661 cgcagcacct tgtcgttaac cagtgaacct ttctgcgata catgagtccc cagaacctgg
    15721 cgcagcgcag cgtgcatcag gtgcgttgcg gagtgattca gacgaatacg ggcgcgacga
    15781 gcctcatcaa catcagcctg cacagcgtcg cccactttca gagaacccgc agcaagttta
    15841 ccgatgtgac caatcgcctg gccgtatttc tgcgtatctt ccaccgcgaa ggagaagtta
    15901 gcgtctttca gttcgccttt atcaccaacc tgaccgccgg attccgcata gaatggtgtt
    15961 tgatctagca cgaccacagc ttcctggcct gcattgatgg catcaaccgc tttaccatca
    16021 acaaacagcg cggtcacttt gccgttcagt tccagatggt catagccttt aaattcagat
    16081 gcactatcaa cacggatcat tgcgttgtaa tcggcaccaa agccgctggc ttcgcgcgcg
    16141 cgacgacgct gctcttccat tgctgcttca aaaccagctt cgtcaacttt gatgttgcgc
    16201 tcacgacaaa catcagccgt caggtcaacc gggaagccat aggtgtcgta cagacggaaa
    16261 gccgtttcac catccagcgt atcaccagaa agttttgcca gctcttcatc cagcaacgcc
    16321 agaccgcgct ccagagtacg ggcaaactgc tcttcttcag tcttcagcac ctgctcaacc
    16381 tgcgcctgct ggcgtttcag gtcttcaccc gcagagccca taacgtcgat cagcggacca
    16441 accagtttgt agaagaaggt ttctttcgcg ccgagcatat tgccgtgacg cactgcgcga
    16501 cgaatgatac gacgcagtac ataaccacgg ttttcattgg acggcattac gccatccgcg
    16561 atcaggaacg cacaagaacg aatgtggtca gcgattacgc gcagcgattt attgctcaga
    16621 tcggtcgcgc cagtgacttt cgctaccgcc tggatcaacg tgcggaacag gtcgatgtca
    16681 tagttagagt taacgtgttg cagcaccgca gcaatacgct ccagacccat accggtatct
    16741 acagacggct tcggcagcgg ttccatcgtg ccatctgcct ggcggttgaa ctgcatgaag
    16801 acgatgttcc agatctcaat gtagcggtcg ccgtcttctt ccggacttcc cggagggcca
    16861 ccccaaatgt ggtcgccgtg atcgtagaag atttcggtgc acgggccgca cggaccagtg
    16921 tcacccatct gccagaagtt gtcagatgcg tatggcgcac ctttgttatc gccgatgcga
    16981 ataatacgtt cgcgcgggat cccgacttct ttttcccaga tttcgtaggc ttcgtcgtcg
    17041 ctttcataga cggtaaccca cagacgctct ttcggcaggg caaaccattt ttcgctggtc
    17101 agcagttccc atgcaaactg aatggcatcg tgtttgaaat agtcgccgaa gctgaagttg
    17161 cccagcattt cgaagaaggt atggtgacgc gcggtgtaac cgacgttttc caggtcgttg
    17221 tgtttaccac ccgcacgcac gcagcgttgg gaagtggtag cgcgggaata attacgcttg
    17281 tcgagcccaa ggaacacatc cttgaactga ttcatcccgg cgttggtaaa caacaaagtt
    17341 gggtcgttat ggggtaccag ggagctgctg gcaactacct gatgtccctt actatggaaa
    17401 aagtcgagaa acgcctgacg gatctcagcg gtgctcttgc tcataattat cctgaaatca
    17461 agctaacgaa atatcgccac cagctccagc gtgtcttaac cgccgggctg gtaactgaaa
    17521 agtgggaata agataagttt tcttgactgg gaagtaaaat accgtatgcg ttcagtcggc
    17581 aaaatttcgc caaatatcct ggatatcttc catcagatag ccacgataga gcagaaaacg
    17641 ctggatctta actttttctg aaaagacagt tggcaaaggt tcatcatatt ttcgcgtcgc
    17701 ctgatcgcgc gccagtgcgc accagtcgat gtcacattca cgcatcgctt tttctgtcgc
    17761 ttcgcgggaa atacctttct gattcagttc ctgacgaata cgtgcaggtc cataaccttt
    17821 gcggctacgg ctggcgataa agcgcgcaac aaatcggctg tcatcgagat agccatgttc
    17881 atggcaccat gcaataacgc gctcgtaatc ttctgccgta gcatcaatct cttctgggcc
    17941 atttttgccc ataatcggtg ccgcgagttt acgtcgcagt tcttgctcac tgtgatcgcg
    18001 caccgccaga atgcgtaccg cacgatccaa caggcgagca tatgccgggc gacgggacgt
    18061 tgattctgtc atggcatatc cttacaactt aaaaaagcaa aagggccgca gatgcgaccc
    18121 aggttttgct gataaacaga tttaatagtc aatgagtgtg cagaatgttc ccgcaggccc
    18181 aattcgttag aggatcgagg tgagagtaat ttttcacgcc agtgctggcc tgttcccctc
    18241 accctaaccc tctccccaaa ggggcgaggg gaccgtccgt gcacacatta tctattatca
    18301 tcaataacga acgattaaaa atcttcgtta gtttctgcta cgccttcgct gtcatctaca
    18361 gagaaatccg gcgttgagtt cgggttgctc agcagcaact cacgtacttt cttctcgatc
    18421 tctttcgcgg tttccgggtt atctttcagc caggcagttg cattcgcttt accctgaccg
    18481 atcttctcac ctttgtagct gtaccacgcg cctgctttct cgatcagctt ctcttttacg
    18541 cccaggtcaa ccagttcgcc gtagaagttg ataccttcgc cgtagaggat ctgaaattca
    18601 gcctgtttaa acggtgcagc gattttgttc ttcaccactt tcacgcgggt ttcgctaccc
    18661 accacgtttt cgccctcttt caccgcgccg atacgacgga tgtcgagacg aacagaggcg
    18721 tagaatttca gcgcgttacc accggtagtg gtttccgggt taccgaacat cacaccaatt
    18781 ttcatacgga tctggttgat gaagatcagc agcgtgttgg actgcttcag gttacccgcc
    18841 agcttacgca tcgcctggct catcatacgt gccgcaaggc ccatgtgaga gtcgccgatt
    18901 tcgccttcga tttccgcttt cggcgtcagt gccgccacgg agtcaacgac gataacgtct
    18961 actgcaccag aacgcgccag ggcgtcacag atttccagtg cctgctcgcc ggtgtccggc
    19021 tgggagcaca gcaggttgtc gatatcgacg cccagtttac gtgcgtagat tgggtccagc
    19081 gcgtgttcag catcgataaa cgcacaggtt ttaccttcac gctgcgctgc ggcgatcacc
    19141 tgcagcgtca gcgtggtttt accggaagat tccggtccgt agatttcgac gatacggccc
    19201 atcggcagac cacctgcccc aagcgcgata tccagtgaaa gcgaaccggt agagatggtt
    19261 tccacatcca tggaacggtc ttcacccagg cgcatgatgg agcctttacc aaattgtttc
    19321 tcaatctggc ccagtgctgc cgccaacgct ttctgtttgt tttcgtcgat agccattttt
    19381 actcctgtca tgccgggtaa taccggatag tcaatatgtt ctgttgaagc aattatactg
    19441 tatgctcata cagtatcaag tgttttgtag aaattgttgc cacaaggtct gcaatgcata
    19501 cgcagtagcc tgacgacgca ccgcatcacg gtcgccgctg aagcattccc gccgggtaat
    19561 gccttcaccg cgggcagtgg caaaagcaaa ccagacggtg ccgacaggct tctcttcact
    19621 gccgccatcc ggcccggcga taccactaat agacacggca taatcagcac gagccgcttt
    19681 cagtgcgcct atcaccattt ccaccacgac gggttcactc accgcgccat gctgcgccag
    19741 cgtctcttcg cgtacgccga tcatctgcgc tttggcttcg ttactgtagg tgacaaatcc
    19801 gcgttcaaac caggcggagc taccggcaat atcggtaatc actttcgcta cccaaccacc
    19861 ggtacaagac tcggcagttg ttacggttgc gccacgggct ttcagcgcct gcccaacctg
    19921 ttcacttaac tgcatcagtt cactgtcagt catcacaacc tctgtcagtc aaaaatttat
    19981 gccggacaag atagcacttt cgtcaggaag atggtgttgg cgttgcaatt ttacgaggag
    20041 gattcagaaa aaagctgatt agccagaggg aagctcacgc ccccctcttg caaatagtta
    20101 ctgcacgcgc gccagcgcca cggcttgtcc taactgccag accgccattg cgtaatgggt
    20161 gctgtggtta taacgggtaa tggtgtagaa gttcggcaga ccgtaccagt actggtaacc
    20221 ggtaccgaca tccagacgca gcaggctggc ttgttgatgg ttgcccagcg gctgctgtgg
    20281 cgttaaacct gccgtggcaa gctgcgaaat gctgtacctg gttttgaagc catttggcaa
    20341 gcctggagcc tgaccgtttg ccattaccgc gacctgatcg cctttcaccc agccgtgcgc
    20401 tttgaaatag ttcgccacgc taccgatcgc atcaaccgga tcccacaggt tgatatgccc
    20461 gtcgccgctg aaatctaccg catattgttt gtaagacgac ggcataaact gtccgtagcc
    20521 catcgccccg gcaaaggaac ctttcagatt gagcggatcg tcctgctcgt cgcgcgccat
    20581 cagcaggaag gtttccagct cgccagagaa atactccgcg cggcgtgggt agttaaatga
    20641 cagcgttgcc agcgcatcga ggatgcgagt tttccccatc acgcgccccc agcgggtttc
    20701 aacgccgata atcccgacga taatttccgg cggtacgcca tacacctgcc acgcgcgatt
    20761 caacgcatct tcatactgat tccagaaaac cacaccgttc tgcacgttgt ccggcgtaat
    20821 aaattttttg cgataacgca gccatgcgcc gttcggacct gatggcggtt tcaccgatgt
    20881 ggttggtgcc tggttatcca tcagccgcag taccgaatcc agacgcttcg cctgggagag
    20941 aatttcctgc aactgctgac gatcgaaacc gtgtttgttc accattttgt cgatgaactg
    21001 ctgggcattc gggttattag cgaaatcgcc gcccatctgc atcacattgt gctgcggctc
    21061 aagcaggaag ccgccagacg gcgttccggt ggtcgtttca gtctcagtag gttttggctt
    21121 gctgctacag gcggcaagca acacaaaaag gggaagcaat gttacataac gacgcttgaa
    21181 catgaggggt ccatttaaca gattcaacca ggggcaagta tggtaaagca tcacgccccg
    21241 cacaaggaag cggtagtcac tgcccgatac ggactttaca taactcaact cattcccctc
    21301 gctatccttt tactcaaact tccaaattaa aatatttatc tttcattttt agattaaaat
    21361 aacactttta aatctttcaa tctgattaga ttaggttgcc gtttggtaat aaaacaataa
    21421 atcctgaagg agagaacaat gatagaaacc attactcatg gtgcagagtg gtttatcggg
    21481 ctgttccaaa agggcggaga ggtgtttacc gggatggtga ccggcattct tccgctgttg
    21541 attagcctgc tggttatcat gaacgcactg attaatttta tcggtcagca tcgtattgaa
    21601 cgttttgctc aacgttgcgc cggtaaccct gtttcccgtt acctgctgct accgtgcatt
    21661 ggcacgtttg tcttttgcaa tccgatgacc ttaagccagg gtcgctttat gccggaaaag
    21721 tacaaaccca gctactacgc ggcggcctct tatagctgcc actcaatgaa tggcctcttt
    21781 ccccatatca accctggcga actgtttgtt tatcttggca ttgccagcgg tctgacaacg
    21841 ctgaacctgc cacttggccc actggcgttg agctatctgc tggttggtct ggtcaccaac
    21901 ttctttcgcg gctgggtgac cgatctgacc accgccattt ttgagaaaaa gatgggcatt
    21961 caacttgaac aaaaagttca cctggcagga gcaacatcat gacgcgtatt cggatcgaaa
    22021 aaggaacggg tggctggggc ggcccgcttg agctggaagc cacgccgggc aaaaaaatcg
    22081 tctatatcac cgccggtacc cggcctgcga ttgttgacaa actggcacaa cttactggct
    22141 ggcaggctat tgacggattt aaagaaggtg aacccgcgga ggcggaaatt ggtgtcgcgg
    22201 taatcgactg tggcggcaca ttacgctgcg gcatctatcc gaaacgacgt attcccacca
    22261 ttaatatcca ctcgacgggc aagtccggtc cgctggcgca gtacattgtg gaagatattt
    22321 atgtctctgg cgtaaaagaa gaaaacatca ctgtagtggg tgatgcgaca ccacaaccct
    22381 cttccgtggg ccgtgactat gacaccagta agaaaatcac cgaacaaagc gatggtttac
    22441 tggcgaaggt gggaatgggc atggggtccg ccgttgcggt gctgtttcaa tctggtcgtg
    22501 acaccatcga cactgtatta aaaaccattc tgccgtttat ggcattcgtc tcggcgctca
    22561 ttggcatcat tatggcttct ggccttggtg actggattgc ccacggtctt gctccgctgg
    22621 cgagccatcc actgggtctg gtcatgctgg cgctcatctg ctccttccca ctgctttcac
    22681 ctttcctcgg cccaggcgca gttatcgcac aggttatcgg cgtattgatt ggcgtgcaga
    22741 ttggtctcgg caatattccg ccgcatctgg ctttaccggc actgtttgcc atcaacgcgc
    22801 aggcggcctg cgacttcatc ccggtcggtt tgtcgctggc ggaagcccgt caggacacgg
    22861 ttcgcgtcgg tgtcccttct gtactggtga gccgcttttt aaccggcgca ccgactgtac
    22921 tgatcgcctg gtttgtctcc ggttttatct atcaatagag gctgaaacat gaccgttatt
    22981 tataagacca ccatcacccg tatcggcgcg agtgccactg acgccctcag cgaccagatg
    23041 ctcatcacct ttcgtgaagg cgcgccagcg gacctcgaag agtattgctt cattcattgc
    23101 cacggcgagt tgaaaggtgc actccatccc ggtttgcaat ttacgctcgg gcagcatcgc
    23161 tatccggtga ccgctgttgg cagcgtggcg gaagacaacc ttcgcgaact gggtcatgtc
    23221 accctgcgct tcgatggttt aaacgaagcg gaatttccgg gcactgtcca tgtggcaggc
    23281 cctgtacccg acgatatcgc gccgggatcg gttttgaagt ttgaatctgt taaggagtaa
    23341 aaaatgaatc aggttgccgt tgtcatcggt ggtgggcaaa ccttaggcgc gttcctgtgc
    23401 cacggtctgg ttgccgaggg gtatcgcgtc gcggttgtcg atattcagag cgacaaagcc
    23461 gcaaatgtgg cacaagaaat taacgccgaa tatggtgaaa gtatggcgta cggttttggt
    23521 gctgacgcca ctagcgagca aagcgttctg gcgctctctc gtggggtaga tgaaatcttt
    23581 ggtcgcgtgg atttgctggt ctatagcgcc ggaatagcaa aagcagcctt tatcagcgac
    23641 ttccagctcg gcgattttga ccgttcgcta caagtgaatc tggtgggtta tttcctgtgt
    23701 gcccgtgaat tttcgcgttt gatgatccgc gacgggattc agggtcgcat tattcagatc
    23761 aactcgaaat ccggcaaagt gggcagcaaa cacaactctg gctacagcgc agcgaaattt
    23821 ggtggcgtcg ggctgactca atcactggcg ctggatctgg cggagtacgg cattacggtg
    23881 cattcactga tgctcggtaa cctgctgaaa tcgccgatgt tccagtcact gttgccacaa
    23941 tacgcgacca agctgggtat caaaccggat caagtcgagc agtattacat tgacaaagta
    24001 ccgctcaaac gcggctgcga ttatcaagat gtgctgaata tgctgctgtt ctacgccagt
    24061 cctaaggcgt cgtactgcac cggacagtcg atcaatgtca ccggcggtca ggtgatgttc
    24121 tgatcaacag cggagatcca ttaaggatct ccgtgagact atagaatgcc tgatgcgcta
    24181 cgctcatcag gcatacagga cttccgccac tacattaagg aaaagttatg gtatccgcac
    24241 tcatcaccgt cgccgttatc gcctggtgtg cgcaactggc cttaggcggc tggcaaattt
    24301 ctcgttttaa ccgtgccttc gacacactat gccagcaagg gcgggttggc gtgggtcgtt
    24361 ccagcgggcg ctttaaaccg cgggtcgtgg tcgccatcgg ctggacgatc agcagcgcat
    24421 cgtcgacacc ttgtttatga aaggactgac cgtcttcgcc cgaccgcaaa aaattcccgc
    24481 aattaccggt atgcatgcgg gtgatttaca gcccgatgtg atctttcccc atgatccact
    24541 atcacagaat gctctatcat tggcgcttaa actgaaacgt ggataatttc gttgtgaatg
    24601 ttacttgctt gcgaagttat cattttgaaa cctaaatcag gtaatcacgc ccatgaaacc
    24661 tcgtcagcgt caggccgcca ttctggagta tctgcaaaag cagggtaaat gctcggttga
    24721 agaattggcg caatactttg acaccacagg cacaaccatt cgcaaagatc tggtcattct
    24781 ggaacatgcc ggaaccgtca ttcgtactta tggcggagtg gtgttgaata aagaggaatc
    24841 cgatccgcct atcgatcata aaacactcat caacacccac aagaaagagc tgattgcaga
    24901 agctgccgtt agttttatcc atgatggcga ttcgatcatt cttgatgctg gcagtaccgt
    24961 tttgcagatg gttcccctgc tctcgcgctt taataacatc acggtgatga ccaacagcct
    25021 gcatatcgtc aatgcgctat ccgaactgga taacgaacaa actatcctga tgccaggcgg
    25081 aacgtttcgt aaaaaatcgg cctcatttca cggacagctg gcagagaatg ccttcgaaca
    25141 tttcagtttc gataaattgt ttatgggcac cgacggcatc gatctcaatg cgggcgtaac
    25201 caccttcaac gaggtttata ccgtcagtaa ggcaatgtgc aatgccgcgc gcgaagtgat
    25261 tttgatggcg gactcatcaa agtttggccg taaaagcccc aacgtagttt gcagtcttga
    25321 aagcgtcgat aagctgatta ccgacgcagg tatcgatccg gcgtttcgtc aggcgctgga
    25381 agagaaaggg atcgatgtga tcataaccgg agagagcaat gagtgaagca ctactgaacg
    25441 cgggacgtca gacgttaatg ctggagttgc aggaagcaag ccgtttaccg gaacgtctgg
    25501 gcgatgattt tgttcgcgcc gccaatatca tcctgcactg cgaaggcaaa gtggtggttt
    25561 cgggaattgg caaatcgggc cacattggta agaaaatcgc cgcaacactt gccagtaccg
    25621 gcacaccggc tttttttgtc catccggcag aagcactgca cggcgatctg gggatgattg
    25681 aaagccgcga tgtgatgctg tttatctctt actccggtgg cgcgaaggaa ctggatctga
    25741 ttattccgcg tctggaagat aaatctatcg cgctgctggc gatgaccggc aaaccgacgt
    25801 caccgctggg cctggcggca aaagcggtgc tggatatctc cgtagaacgc gaagcctgcc
    25861 cgatgcacct tgcgccgacc tccagcaccg tcaataccct gatgatgggt gacgcgctgg
    25921 cgatggcggt catgcaggcg cgcggattta atgaagaaga ttttgcccgc tcccacccag
    25981 ccggggcact gggcgctcgc ttgctgaata aagtgcatca tctgatgcgc cgtgacgatg
    26041 ccatcccaca ggtggcgtta accgccagcg tgatggatgc gatgctggaa ctcagccgca
    26101 ccggtctggg gctggtggcg gtatgtgacg ctcaacaaca ggtacaaggc gtctttaccg
    26161 acggcgattt acgtcgctgg ctggtaggcg gcggcgcact caccacgcca gttaatgaag
    26221 cgatgacgac aggcggcacc acgttacagg cgcaaagtcg cgccatcgac gccaaagaga
    26281 tcctgatgaa gcgcaaaatc actgccgcac cggtggtgga tgaaaacggc aaactcaccg
    26341 gcgcaattaa cctgcaggat ttctatcagg ccgggattat ttaatccttc attcccagac
    26401 gtttcgccag ccgatgcagg ttggcgacgt cggtttccag catccgcgcg caggcagccc
    26461 agttatgatg attttgtgcc agtgcctggc gaatagtttc acgctggaac gcttctgtcg
    26521 cttcacgcag gttttgctta acaacgggca ccgccgccgc ttctggcggc ggcaacgtca
    26581 cctcaggaaa agcaaaatgt tgcgcctcaa gaatcacttc atcgccgttg cgggtggctc
    26641 tcgccagcac taccgcccga tgaatagcat gttccagttc gcgcacgttc cccggaaaac
    26701 gatagtgttg cagtaaattt cgcgctccgg cacttaatac cacgcgggag agccccagcc
    26761 gcaaacgaca ctgttcgcag aaataccccg ccagcagaat gacatcatcg ccccgctcac
    26821 gcagcggcgg caccgaaagt ggaaacacgc tcaggcgatg aaacaagtca gcgcgaaatc
    26881 gccctgccag cacctcttcg cgtaagtcgc ggttagtcgc cgccagcacg cgcacatcga
    26941 cccgcaaact acggtcatcg ccaacgcgct gaatatcgcc atactgcaac accctcagca
    27001 gcttggcctg caatgccaac gacaactcgc cgatctcatc cagaaacaga gtgccgttat
    27061 ccgccatttc aaacttcccg ctgcggttac tgatagcgcc agtaaacgct cctttcacat
    27121 gcccgaacaa ctcactttcc gccacacttt ccggcagtgc agcacagttg agatagacca
    27181 gcggattcac cgcccgtggc gaggcttcat gaatcgcttt cgccaccagc tccttaccgg
    27241 ttcccgtctc accgctgatc aggacgttga gatcggacgc cgccacaatc tcaatctctt
    27301 ttttcagttg cgtcatgcca ggggacaagc caatcatctg cgtctgtttc accgcttcaa
    27361 acggcgtggc atcgcctggc atcatattct ggctttccag ttgttcaatc agcaacgcat
    27421 tgcttaacgc tcccgccgcc agcgcagcaa tcagccgtag ctcttcgtcg ctgaaaacat
    27481 cgaactgatc gggctgcatc ccgtcgagcg tcagtgcgcc gatcaggttt tgcccggcaa
    27541 acaatggcag accaacgcag gcgtgaacct tcagactctc ctgcccagga atcaaaccgt
    27601 catagggatc gggcaattcg ctgtctgcgg gaaagcgcac cacatccccg gcgcgggcaa
    27661 tcgcttccag ccgtggatgc ccttccagcg caaagcgtct accgagtaca ccctttgcca
    27721 gaccgtcgat ggcaagcgga ataaactgcc gcgaatcgta acgtagcaac gcagacgcat
    27781 cgcactccag cacctgacgt agcgtggtga tcaggcgctg aaaacgatcc tgatgaccaa
    27841 tcccacgctg caattcgatg gcgatattcg ccagcacatc aacggaaaaa ctcatctttg
    27901 cctcactgtc aatttgacta tagatattgt catatcgacc atttgattga tagtcatttt
    27961 gactactcat taatgagcat aattttattt atagagtaaa aacaatcaga taaaaaactg
    28021 gcacgtaatc tgcaattagc aagacatctt tttagaacac gctgaataaa ttgaggttgc
    28081 tatgtctatt gtggtgaaaa ataacattca ttgggttggt caacgtgact gggaagtgcg
    28141 tgattttcac ggtacggaat ataaaacgct gcgcggcagc agttacaaca gctacctcat
    28201 ccgcgaagaa aaaaacgtgc tgatcgacac cgtcgaccat aaattcagcc gcgaatttgt
    28261 gcagaacctg cgtaatgaaa tcgatctggc ggatatcgat tacatcgtga ttaaccatgc
    28321 agaagaggac cacgctgggg cgctgaccga actgatggcg caaattcccg atacgccgat
    28381 ttactgtact gccaacgcta tcgactcgat aaatggtcat caccatcatc cggagtggaa
    28441 ttttaatgtg gtgaaaactg gtgacacgct ggatatcggc aacggcaaac agctcatttt
    28501 tgtcgaaacg ccaatgctgc actggccgga cagcatgatg acttacctga caggcgacgc
    28561 ggtgctgttc agtaacgatg ctttcggtca gcactactgc gacgagcatc tgttcaacga
    28621 tgaagtggat cagacggagc ttttcgagca gtgccagcgt tactacgcca atatcctgac
    28681 gccgttcagc cgcctggtaa caccgaaaat taccgagatc ctgggcttta acttaccagt
    28741 cgatatgata gcgacctctc acggcgtggt atggcgcgat aacccgacgc aaattgtcga
    28801 gctgtacctg aaatgggcgg ctgattatca ggaagacaga atcaccattt tctacgacac
    28861 catgtcgaat aacacccgca tgatggctga cgctatcgcc caggggattg cggaaaccga
    28921 cccacgcgtg gcggtgaaaa ttttcaacgt cgcccgaagc gataaaaacg aaatcctgac
    28981 caatgtcttc cgctcaaaag gcgtgctggt cggcacttcg acgatgaata acgtgatgat
    29041 gccgaaaatc gccgggctgg tggaggagat gactggttta cgcttccgta acaaacgcgc
    29101 cagtgctttc ggctctcacg gctggagcgg cggtgcggtg gatcgtcttt ccacgcgcct
    29161 gcaggatgcg ggtttcgaaa tgtcgcttag cctgaaagcg aaatggcgac cagaccagga
    29221 cgctctggag ttatgccgtg aacacggtcg cgaaatcgcc cgtcagtggg cgctcgcgcc
    29281 gctgccgcag agcacggtga ataaggtagt caaagaagaa acctctgcca ccacgacggc
    29341 tgacctcggc ccacggatgc agtgcagcgt ctgccagtgg atttacgatc cggcaaaagg
    29401 cgagccaatg caggacgttg cgccaggaac gccgtggagt gaagtcccgg ataacttcct
    29461 ctgcccggaa tgctccctcg gcaaagacgt ctttgaagaa ctggcatcgg aggcaaaatg
    29521 agtaacggca ttgtgatcat cggttcgggc ttcgccgccc gccaactggt gaaaaatatt
    29581 cgcaaacagg acgccactat tccattaacc ctgattgccg ccgacagcat ggatgagtac
    29641 aacaaacctg acctcagcca tgttatcagt caggggcaac gtgccgatga ccttacccgc
    29701 cagacggcgg gtgaatttgc cgagcagttt aatctgcacc tgtttccaca aacctgggtg
    29761 acggatatcg atgccgaagc ccgtgtggtg aaaagccaga ataatcagtg gcaatacgac
    29821 aagctagtac tggcaaccgg tgccagtgcc tttgtcccgc ctgagcctgg gcgtgagtta
    29881 atgctgacgt taaatagtca gcaagagtat cgcgcctgtg aaacgcaact gcgggatgcc
    29941 cgacgcgtgt tgattgttgg cggtggtttg attggtagcg aactggcgat ggatttttgt
    30001 cgtgcaggca aagcggtcac gctaatcgac aacgctgcca gtattctggc gtcgttaatg
    30061 ccaccggaag taagcagccg cttgcagcat cggttgacgg agatgggcgt tcatctgctg
    30121 ttgaaatctc agttacaggg gctggaaaaa acggattctg gcattctggc aacgctggac
    30181 caccagcgta gtatcgaagt ggatgcggta attgccgcca ccggactgcg cccggaaaca
    30241 gccctggcac gacgcgccgg gctgacgatt aatcgcggcg tttgcgtcga tagttatctg
    30301 caaaccagta atgacgatat ttatgcgctg ggcgattacg cggaaatcaa cggtcaggta
    30361 ctgccattcc tgcagccgat tcaacttagc gcgatggtgc tggcaaaaaa tcttctcggc
    30421 aataacacgc cgctgaaact accgacgatg ctggtgaaaa tcaaaacgcc ggaattaccg
    30481 ctgcatctgg caggcgaaac ccagcgtcag gatttacgct ggcaaattaa taccgaacgc
    30541 tagggaatgg tggctcgcgg tgttgacgat gctgaccagc ttcgcgcctt tgtggtcagt
    30601 gaggatcgga tgaaagaggc atttggattg ttgaaaacat tgccgatgta ggtgggctac
    30661 tgtgcctaaa atgtcggatg cgacgctagc gcgtcttatc cgacctacgg ggacgcatgt
    30721 gtaggccgga taaggcgttt acgccgcatc cggcaatggt gtccaaatgc aacacgtttt
    30781 atccgttctg gacttcaccc gctaaccaac gcgccgcagc aataaccccc tgccccagag
    30841 acaaaccgcc atcacccgcc ggtaaactct gtggaaagag caatgtgaaa tcagcgagat
    30901 aatgcgccag acgtgcacgc agcaaacggt tatgaataac cccgccgcta aataccagcg
    30961 tagtgatacc acgcatcgtg gcctgctcac gcatcaacgc ggcaaaaccc tgcgccagcg
    31021 catcatgaaa cgcccacgcg cgttgattaa cctgtgcctg ccagttcagc cactgctgcc
    31081 agaaagcggc gagatccagt tgattgtcca ccagcggcat cgtcaccgga tgcgtcactc
    31141 cgtggcatga ggctgcgagc gcctccagag cacaagccgc ttcaccttca taacttaacg
    31201 tggctggcgc acagcccagt gccgccgcca ctgcatcgaa caaccgccca cacgatgacg
    31261 ccagcggcgc gttaattcca cgctcaatgg cccgcgccag cacgctccag ttttgctgtt
    31321 gcacacttgc tgtttcaggg taattctgcc actccggcac aaagcgcagg cactgcgcca
    31381 gcaggtttcg ccacggctgc ttcgctgcca aatcgccacc cggaagcgcc actgcaggca
    31441 agccgcccag gtgctcacat tcgcgatagt tcacccgcag gcactcgccg ccccacaaag
    31501 cgccgttctc ccccataccg ataccgtcga gcgtcaaagc aatgacatca ccgccatcca
    31561 gcggccactg atgctctgcc agacacgccg ctgcatgggc atgatgatgc agtaccgttt
    31621 gcgtcggcag attcatttca cgcgcccact ggctggagac atagcccgga tgcgcgtcat
    31681 gcacaacgta tttcggggta aaatcgtaga tgttttgcat caggcgtaac gcttcgcgcc
    31741 actgcatctg gatgccatcg tcacttaaat cgcccagatg ctgactcaac accgcttgtt
    31801 caccgcgcac caggcagaag gtatttttca gatccgctcc gagacacagc acaggcggaa
    31861 catttttaaa gcccggaggc aaagccagcg catccggcac atacccccgc gaacggcgca
    31921 gcatttcgcc gctttcgcgc accaccgaat catccatccg ctgcacgatg tcgcggttat
    31981 gtatcaagaa tccgtcggca atgccctgca aatccgccag cgcctgttcg ttgctgatag
    32041 ctggtggttt accgctcagg ttgccggagg tcatcaccag cgggcattgc agttcctgta
    32101 acagcaaatg ctggagcggg ttcgcaggca acattacccc gacttcgtta aggtcagggg
    32161 cgatatcatc acaaagctca ggaacgtatt ttttatccac cagcacaatc ggcgcggcgg
    32221 gcgtggtaag caactggcgc gcagcgtctg gtaaaccgtc agccactggc aacatgaccg
    32281 ccagcggttt cgccgggcga tgtttgcgcg cccgaagtgt cgccaccgcg ttactgttac
    32341 gtgcatcgca ggcaagatga aatccgccaa tccctttgat ggcgacaatg ttgcccattt
    32401 ttaactgtgc gatagccgcc tgtaatgccg cctcttgttc tgcatgttca ccatgactta
    32461 cccattcaag atgcgggcca cactccgggc aggccaccgg ctgggcgtgg aagcgacgat
    32521 cgagcgggtc gcggtactct ttgtcacaag ccggacacaa cggaaacgcc gccatcacgg
    32581 taaacgggcg gtcgtaaggc atggcgcgaa taatggtgaa acgcgggccg cagtgggtac
    32641 agttgataaa cggataacga taacgccgtt cgcctggggt attcatttcg gcaaggcaag
    32701 cagggcaagt agcggcatcc gggacaattt gcgtattcat ggcacctccc gcgctctggc
    32761 ggatggtgaa ctcagtgggc agttgtgacc agatgtacgg ctcacgctcg acgctatcaa
    32821 tacgcgccag cggcgggcag tgctgatgca attgaacaag aaacgtttcc gggtcttccc
    32881 gcagccggac ttctacgcca tcgccgtcat tacagacatc gccgtgaaga tttaattgct
    32941 gtgccagctg ccagacaaac ggacgaaaac cgacgccctg cactttgcca cgaatacgca
    33001 gttggacacc gcaagatgtg ttttttgcca ttgagttatt cccgccatca tgaattgcgt
    33061 aacccgccca gccggacacg acagcgtcgc atccggcagt cacaggtcgg cgataccgcc
    33121 cgctccgtat tctacgaata tttccgggaa ttcctttgat gccagaacag ttctgtaaga
    33181 tttttagaac atcagcgccg tacggcggcg tttttctgca ctcagttgtt caagtttatt
    33241 acgatcgaca caaatcagcg catgagtcgg gcaagccgcc atacacgccg ggccgtcttc
    33301 acgatggttg cacaggtcac atttattggc ttcggctttg tcagcccgta cattcagccc
    33361 cgcgccgctg ttgcgaatca ccggacgtac caccacttcc atcgcgccat acgggcaagc
    33421 cacaacgcag gttttgcaac caatgcaacg ttcctgcatc acatgaacaa accctttatc
    33481 acggctgata gcaccattcg ggcagacgtt agcgcacggt gcatcttcac actgacggca
    33541 aactgtcgcc gtggaaatgt tcacaccttt aatgacatgg atacgcggta aaaatgtttc
    33601 cggggtcagc gatgcacagt cctgattttc ctgatgagaa accacgcacg ccacttcaca
    33661 ggtacggcaa ccaatacatt tactcgcgtc agcaatgatg aaacggttca tcaaattctc
    33721 cagcaatgac agttaatgcg ccgatacatt cacaaatcat gccagttttt aattcactgt
    33781 tatttaagga aattaatttc tgtaatgcag gaaaaaagat gtcatcgaca ctagtgacga
    33841 tgacatgtga tgacaatgtt tatcgcgaaa gagcaatgag tgagtcgcgg tggatcagtt
    33901 ttccgctgaa ggttttcggc ggtgagaaat ccccgccatc gagcataaaa atcagccgtc
    33961 caataatttc ctgaatcatc tcagtcaccg gaatttttac gctggagagc gccggaacga
    34021 tatagggggc aattgcgata tcatcgaatc cgataactga cacctgctct ggcaccgcta
    34081 cgccgcgctc gtgcaacgct ttcatcgcac ctatcgccat atcgtcgtta ctggcaacta
    34141 acgcgctaaa tttagcccca cgttcgagca acgtttctac cccttcggcc ccgctggcgg
    34201 gcgtccattt accgttagcg ataagttttt cattgagcgc aataccatgc tgcgccagcg
    34261 cgtctttata cccagcaaga cgttcaatgc tggtggggga atccatcgag ccggtaagga
    34321 aagcaatctc ctgatgtccg gcgtttatca actctgccac ggcattaaag ctggtctgtt
    34381 tatgatcgca ccagacgcta tggctgctgt ttttgcgcag gcggcgatta agcaccatta
    34441 tcggctgact gtgcgcgtca atgatgtcat cgatctcatc cacgcttaaa aaacgcgggt
    34501 aaatcataat ggcgtcgcag cgcaaatcca gcagatactg aatcgcctgg cgctcttctt
    34561 cggcgctgtg tttaccatct gccaatagca actgccgccc tttctcttcc gccattcgcg
    34621 cggcatgaaa gagtaattca ctaaaataaa tgccgtggta aagcgtgttg gtcactacca
    34681 gccccagcgt ctgagtactc ttcgccgaca gattgcgcgc cagcaagttt gggcggtaac
    34741 cgctctcttc taccgcctga aacacgcgat ctttagtctc ctggctgacg tagccattac
    34801 ctgaaagcac gcgggaaacg gtcgcttttg aaaccccggc gcgcttcgcc acttccagca
    34861 tcgtcgtcat cattttcatc cctttacacg caatcaacgc agtgtactgc accgtttgcc
    34921 gattgtcctt gcgcaatcgg cgggaaaaat attcaggtga ccggtttcac aaatataaaa
    34981 aatgaataat taactctctt gcttatttag tgacaactat tcatgatttt gtgaaaccgg
    35041 tttcctaatt ccgtttcagc atcggcattt ttccgttacg tcgactgata acaactacat
    35101 ctaccctact gataacagga taaaatccga tggccaaaaa ttatgcggcg ctggcacgct
    35161 cggagatagc ggcactgggc ggcgttgata acatctcggc ggtcacgcac tgtatgacgc
    35221 gtttgcgctt tgttatcaaa gatgaccagc ttatcgacag cccgacgtta aaaaccatcc
    35281 ccggcgtgct cggcgtggta cgtagtgaca accagtgtca ggtgattatc ggcaataccg
    35341 tttcacaagc ctttcaggaa gtcgtcagcc tgctgccggg agatatgcag cccgcacagc
    35401 ccgtgggtaa acccaaactc acgccgcgtc gcattggtgc ggggatcctc gatgcgctga
    35461 tcggcaccat gtcaccgctg atcccggcga ttatcggcgg atcgatggtc aaactgctgg
    35521 cgatgatcct cgagatgagc ggcgtgctga caaaaggatc gccgacctta accattctga
    35581 atgtgattgg tgacggtgct ttcttcttcc tgccgctgat ggtcgcggca tctgccgcca
    35641 tcaaatttaa aaccaatatg tcgctggcga ttgccatcgc gggtgtgctg gtacatccga
    35701 gttttattga actgatggcg aaagcggccc agggtgaaca tgtcgaattt gccctgattc
    35761 cggtcaccgc ggtgaaatac acctacacag tgatcccggc gctggtgatg acctggtgcc
    35821 tgtcatatat cgaacgctgg gtggatcgca ttacaccggc ggtgaccaaa aacttcctca
    35881 agccaatgct gattgtgttg attgccgcac cgctggcaat cctgctgatt ggcccgattg
    35941 gtatctggat cggtagcgcc atttcggcgc tggtttacag cattcatggt tatctgggct
    36001 ggctttcagt cgccattatg ggcgcgctgt ggcctctgct ggtaatgacc gggatgcacc
    36061 gcgtctttac gccaaccatc attcagacca ttgccgaaac cggcaaagaa gggatggtca
    36121 tgccgtcaga gatcggcgct aacctgtcgc tgggcggttc atcactggcg gtggcgtgga
    36181 aaacgaaaaa cccggaactg cgccagacgg cgctggctgc ggcggcatca gccattatgg
    36241 cggggatttc cgaaccggcg ttatacggcg tggcgatccg cctgaaacgt ccgcttatcg
    36301 ccagtcttat cagcggtttt atttgcggcg cggttgccgg tatggcgggg cttgccagcc
    36361 actcaatggc agcgccgggg ctatttacca gcgtgcaatt cttcgatccg gcgaatccaa
    36421 tgagcatcgt ctgggtgttc gcggtcatgg cgctggcggt ggtgctgtcg tttatcctca
    36481 cactgttgct cggctttgag gatattcctg ttgaggaagc ggctgcccag gcgcgaaagc
    36541 atcagagcgt acaaccgacc gtcgccaaag aagtaagtct taattgagga tgaaaatgtc
    36601 agtatttcca gaaggttttt tatggggcgg cgcgcttgcc gccaaccagt ctgaaggtgc
    36661 gttccgtgaa ggcggaaaag ggctgaccac cgtcgatatg atcccacacg gcgagcatcg
    36721 aatggcggtg aaactggggc tggaaaaacg ttttcagttg cgcgatgacg agttttatcc
    36781 cagccatgag gcgacggatt tttatcatcg ttataaagaa gatatcgccc tgatggcaga
    36841 gatgggattc aaggttttcc gtacctcaat tgcctggagc cgtctctttc cgcagggcga
    36901 tgaaatcacg cccaatcagc agggcattgc tttttatcgt tctgtctttg aagagtgtaa
    36961 aaagtacggt atcgaaccgc tggtcacgtt gtgccacttc gatgtgccga tgcatctggt
    37021 caccgaatat ggctcctggc gtaaccgcaa gctggtggag tttttcagcc gctacgccag
    37081 aacctgcttt gaagcatttg atggtctggt gaaatactgg ctaaccttca atgaaatcaa
    37141 cattatgttg catagcccgt tctccggcgc gggtctggtg tttgaagaag gtgaaaatca
    37201 ggatcaggtg aaatatcagg ccgcgcatca ccagctggtt gccagtgcgc tagccgccaa
    37261 aatcgcccat gaggttaacc cgcaaaatca ggtgggctgt atgctggcgg gcggcaactt
    37321 ctacccttac agctgcaaac cggaagatgt ctgggcggcg ctggagaaag atcgggaaaa
    37381 cctgtttttt atcgatgtgc aggcgcgcgg cgcgtatccg gcttactctg cccgcgtatt
    37441 tcgcgaaaaa ggggtaacca tagataaagc gctgggcgat gatgagatcc tgaaaaacac
    37501 cgtcgatttt gtctctttca gctattacgc ctcgcgctgc gcctcggcgg agatgaacgc
    37561 caacaacagc agtgcggcga acgtggtgaa atcgctgcgt aacccgtatc tgcaagtgag
    37621 cgactggggc tggggaattg atccactcgg tctgcgtatc accatgaata tgatgtacga
    37681 tcgttatcag aagccgctgt ttctggtgga aaacggcctc ggcgcaaaag atgaacttgc
    37741 tgccaatggc gagattaacg atgactatcg catcagctat ttacgcgaac atatccgcgc
    37801 aatgggcgaa gcgattgcag atggcattcc gctgatgggc tacaccacat ggggctgtat
    37861 tgatttagtt tccgcctcta cgggtgaaat gagcaaacgc tacggttttg tctacgtaga
    37921 ccgtgacgac gcaggcaacg gcacgctgac gcgcacacgt aagaaatcgt tctggtggta
    37981 taaaaaagtg attgccagta atggggaaga tttagagtaa gtaacagtgc cggatgcggc
    38041 gtgaacgcct tatccggcct acatgtcgca cgaaccagga aggcccgata agcgcagcgc
    38101 atcgggcaat ttagcatttg gcatcagagc tggttcaccc catcaagaac atccctgtcc
    38161 tgattcctta atgaaaaact actcttcttc caccgctaac tgcgcgaagc cgccattccc
    38221 ttcccagcct tccagtcgtt gataaacggt ttctaccgca tctttaatcg gctgggtcat
    38281 cgggtagtaa aagccgacga tatccggctg tatgccgagg aaaatcactt cgccaatatc
    38341 ttctttcaac tggtcgataa gatagttcag aggcatgtta tgggtagtca tcataaacat
    38401 ctcggcgata tcatccgggt cgatgatgcg gatctcgccg gggtttagcc ccatatccgt
    38461 ggcgtcgaca atcagtaacc gcgtagggcg cagttcacgg atggcgacga tgtcgttttc
    38521 cggtgcacta ccgccgtcaa tcaccaccca gttacctttc ggcgcggcgg cgcacttttc
    38581 cgccagcagc ggacctgcgc catcatcgcc catcatgcta ttgccaacac agagtaaaac
    38641 gtcagtcacg caatctcctc accatcaaat agatggcgtt ttcctgatga atatcatgca
    38701 gcatacttaa cagcattgta ctccattcct gctgctgcga agtttgcgct gcccgggctt
    38761 tatcgaatgc gttggccagc atcggcacat ggttaatgtc gatgacgatc tcaccgtact
    38821 tcggtacgcc ttccattttg cggcgggctt cactgcccgc ctccagagtg gcaatccatg
    38881 ccagatattc atcccacggg caggtgagcg ccgcttccag gcaatcgata accccaaggt
    38941 ggtgaccaat cgccaggctg taatagacca cctgctgcgc ctcggcgggc gtggcatcgt
    39001 tctcatcaat aaatttacgg ctcagttgac tgaacaccac cttttcactc atcggatacg
    39061 cacctcttct acaacatgat tcagatggct gacaatctcg ttcagacgcg gatcgttttc
    39121 cgcttccagc cagcgtgcaa cctgttcttc gccctgccct aactgtgtca ggaagtcatc
    39181 ggcaatctga cgaccgtaac gataacccgc caggcggcgt gcttcgcgat ccactttcac
    39241 gcgcagcggc tgcaccatat cgccatgcag gatctccgcc ggttgttcat ccagttcacc
    39301 cggcccacgg gcgtgaattt tctgctccag caggccgagc gccattgcaa agccgtacag
    39361 cgtggcggca ggcgttggcg ggcagccagg gatataaaca tccacaggga caattttatc
    39421 cgtaccgccc cacacgcagt agagatcgtg gaagatcccg ccgctgttac cgcaggcacc
    39481 gtaggagata caaattttcg ggtccggcgc ggactgccac gcacgcagcg caggggatcg
    39541 cattgcacgg gtgaccgcgc cggtaaacag taaaatatcc gcatgacgcg gtgaaggaac
    39601 gactttaatg ccgaagcgtt ctgcatcaaa cagcggcgat aaagtggcga aaatttcgat
    39661 ttcgcagccg ttgcagccgc cgcagtccac gcggtaaaca taggcagaac gtttgatttt
    39721 tttcagtaac gacgccttca tgctggcgat ggattcatcc accgtcatcg ggaccggaat
    39781 gccgttggcg tcacgggggc ctaataaatt gctcatcaga tggcctcttt catatggcga
    39841 gtcagttcaa tacggtcgga cggcaccagg catttctggc gcttacattc cgggcaagtc
    39901 tcaaagcttt cgcggtggtt ttccgcgcgg ctgtcgccgt tgtgcttaag cagcgcaatg
    39961 gcgtagtcga tctctttctg gacggcgaaa ggacgattgc agacgcgaca gttgcacagc
    40021 gcgaagcgag actgttgcag gaagtcttct ttcttccaca ccgccagttc gtactcttgc
    40081 gacagcttaa tcgccgccgt cgggcaaact tcttcgcagc gtccacagaa gatgcagcgc
    40141 ccgagattaa actcccaggc cagctctcct gtggcgaggt cagtttcaac cgttaaggcg
    40201 tttgacgggc aggcattgac gcaggccgcg cagccgatgc actgctgcgg gttctgctct
    40261 ggcttaccac ggaagttttt atcaaccgca atcggctcca gcggataaga cgaggtcgcc
    40321 gtaccggttt tgataacttt tttgataaag gtaaacatgg cgattcctta tttcagcggc
    40381 gagtttttac gctcaatgct gtagcgctcg agttctttgt acggcaccac tttgctcttc
    40441 ttcttacgta catcgaccac agtcatgcgg tcggtacagg agtagcaagg gtccaggcta
    40501 ccgataatca gcggcgcatc ggaaacagtg ttgccacgca gcatgtagcg cagggtcggc
    40561 cagttcgcgt aggtcgcggc acggcagcgc cagcggtaca gcttctggtt gtcgccagtc
    40621 atgctccagt ggatatcatc accgcgcggc gcttcggcaa agcccagcgc gaaacggtgc
    40681 ggaatgtagg taaagccttc caccatcagc gggccacccg gcaggttatc cagaccgtag
    40741 tcgatcatgt tcagcgcggt atagacttcg ttgatacgca ctttcagacg cgaaataacg
    40801 tcgcagccct gctcgctgtg gacttccatt ggcagcaggc cataaccaac aaacgggtga
    40861 tcagcgcggg tatcgcgagc gtgtccgctg gcgcggacca tcgggccaac gttactgaaa
    40921 tcgcgggcaa tttccgggtc cagacgacca atgccgacgg tgcgctgttc catgttcggt
    40981 gtgctcagca gcacatccac cagctcctgc acttcacgac gcatctgttg cgccagctgg
    41041 cgggtctgga tcatgtcatc tttcaacaga tcgcgacgaa tcccgccgat caggttcagg
    41101 ccgtaggttt tacgcgcacc ggtgaggatc tcggccattt tcatggaggt ttcacgcacg
    41161 cggaagaatt gcataaagcc ggagtcgaag ccggtaaagt ggcaggcgag gccgaggttg
    41221 agcagatgcg agtgcaggcg ttccacctcc agcagaatgg cgcggatcat ctgcgcacgt
    41281 tctggcacct gaatacccat cgcgttttcc accgacgtgg tgtaggcggt gctgtgggcg
    41341 aagccgcaga tcccgcacac acggtcagag aggaaggtca cttcgttata acccatgcgg
    41401 gtttccgcca gtttttccat accgcgatgg acatagaaca agcggtagtc tgcgtcgata
    41461 atgttttcgc catcgacgaa cagacggaag tggcccggtt cgtcagaagt gacgtgcagc
    41521 ggaccaatcg gcacgacgtt gtttttcttg tcgcccagtt cgttgatgaa ctcgtaggtt
    41581 tcagcatcgg tggtcggtgc cggacgctga cgataatcca tgctgtcttt acgcagcgga
    41641 taaagttcat ccggccagtc atccggcagc accagacggc gttcatccgg cagaccaacc
    41701 ggaatcaaac cgtacatatc gcgcacttcg cgctcgcccc acaccgccgc cggaacgcgc
    41761 ggcgtcacgg acggatactc cggtttgttg gcatcaactt cgacgcgaac cgttacccag
    41821 cacttagtcc ccttctccat cgacagcacg taataaacgg cgtaatgacc attcagtttg
    41881 cgttcgtcgt taccaaaaag caccgacagc cagcccccct gcttgtagta gagaaactcc
    41941 accacttccg gcagatagtt cacctttacg gtgacagtca gctgctcttt ggtctgccag
    42001 gcgtggtcca gcacgacgcc cggaaatgcc tcattcagcg cggcgagata atgttgacct
    42061 aatttttctt cagacatgct caaactctct ttaatcacgc cgccagcaag gagacgaacg
    42121 ctaaaaatgc aaagccaaac ccagcccagg taatgcgcga agtaatatca agacgcagac
    42181 gggccatgct gttttcgaac agcgcgataa ccaggacgcc gaccaccagt tttacgatcg
    42241 caatcaccag cgccagcagc agtccaccgg cggtgaaggt ttccatttgt ccccacggaa
    42301 taaacacccc gacgaacatc tgcaacacca ccagctgttt caggctgata ccccatttca
    42361 tgacgccaaa gccgctgccg ctatactcag agagcggacc ttcctgcaac tcctgctcgg
    42421 cttccgccag gtcgaacggc agtttgccca tttcgataaa agtggcgaac gcacaggcgc
    42481 agagcgccag caccagcggg atgctttggc tcagcggcca gtgataaacg gtgtcggtaa
    42541 tgttgctgat gttggtggaa ccggcaacct gtgcggcaac ccacagacca agcagcaaca
    42601 tcggttcgac cagcacgcca agcatcgctt cacggctcgc accgatagcg gtaaacgggc
    42661 taccggtatc cagaccggaa atggcaaaga agaaacgcgc gatggcgaag agatacagta
    42721 aggtgatcaa atcgcccagt tgcggcagcg gagaaccgac ggtcaccacc ggcagcgcag
    42781 tagcgatagt cagcatgacg cccaccatca catacggcgt caggcggaac acccagccag
    42841 aggcatccgg gccaacgctc tggcggccca gcagtttgat aatgtcgcga tactcctgca
    42901 acacgcccgg cccgcgacgg ttatgcagac gggcgcgcgc cacgcgggta ataccggaga
    42961 gcagcggcgc aacggcaaat aacaccagcg cctgaattaa cggatataaa acactcattc
    43021 tcaggctcct cgtgaaacaa taatcaccac cagtaccgcc agttcaacca gcgccatccg
    43081 gcggaacagc aacgcactac cttcgcactg ccagcccggc accagcgaca ccggattcag
    43141 ccatttgcgc agtttcagca ctggcgcaaa cgcctgttta accggcatgg caaaaccgtg
    43201 agcggtaatc accattgatt tttcatggtc gtagccgcac acccaggccg caccgcggga
    43261 acgtgacggc aggcggtcgc ctttgcaaat cgccataatg atgaatggca gcagcgggca
    43321 ggcaatcagc agcaatgtga tcattggttg agaaacggtg gtgttagcag gctccagagg
    43381 cagaggtacg gcagcagaga gcatcggcag cagccacggc gcagcaacac cgccaattac
    43441 gcagcaaatc gccagtgcca ctacgcttac gctcatcagg agcggcgcac aggtggcgtt
    43501 ttcggcttct ttggtacgcg gcgcgccgag gaacgtgacg ccatagactt tcgccataca
    43561 catcaccgcc agcgcaccgg taattgccag acccacagcg agcagcggcc ccagcagacg
    43621 ggcaacaaac gcgccactat tgctcagttt gaaaaaggat tgatagataa cccattcccc
    43681 ggcaaacccg ttcagcggcg gcagcgcagc cattgccatc aacccgacta acatggcgat
    43741 ggagataacc ggcattttct tgccaatacc accgagtttt tcgatatcgc gatgaccggt
    43801 acggaaccag acgctccccg ccccgaggaa cagtacgctt ttgaacaggc tatggttaag
    43861 cagatggtac agaccaccga ccaggccaag agcaatcagc gccggttgtt cgagcgcgat
    43921 acccgttacg ccagcgccca gccccagcag gataatgccg atattttcca gggtgtggta
    43981 agccagcagg cgctggatgt tgtgctccat cagcgcatac agaccaccga caaacgcggt
    44041 gatcatgccg agcaccagca gcacgatccc ccaccacagc ggtgcattac cgcccagcag
    44101 tgacaggatt aaaatgccca gcaggccaat tttcatgact accgtagaaa acaacgcggc
    44161 agctggcgca gaggcgttag catgtgcctg cggcacccag ccgtgcagcg gaataatccc
    44221 ggcgagcagg ccaaagccaa tcacaccgag cagccagata tcggaaccga gcggcagctg
    44281 ttgcatacgc atatccagca ggcgcagatc cagcgtgcca taacgctgcc acagcagcca
    44341 gcaagcaatc gccagcagca gagtgccaag acgccccagc gcaaaccaca gtttgccctc
    44401 tttgctgttg ctggtgagga acaccgcaca caaggccatg acttcggcca ttaccacgaa
    44461 catgccgagg ttgctggcga tgacggcgca gacggcagca gccatcaaca tattgatctg
    44521 caagccgttg cacttcacct gcgcgtggcg atgccagtca atgttgtaga ggctgacaaa
    44581 cagaccgcac agaccgagcg taatcagcca aatcgcgtta agcggggaga tttgcacatc
    44641 gtagcttacc agcgacagcg caccgctcac gccaaccgcg ccagtcagca cagtgaagcc
    44701 cgcggctgcc gtatacaggc taccaaccgc gccgccaatt ccggctatcc agccacttag
    44761 cgtcttttga aaagaaaaga gaaatgccag aacagcggcg gcgacaaacc acaccacgcc
    44821 gctattgatc agggaaattg cgctcattta gcctctccac tttgagcctg ctgaaacaag
    44881 gtgagatcgc caaagtccgt attaaaagtc agctcacgct tacgtttgct gacgcgggcg
    44941 atatcggtgt tatccaccag atgcagggct ttggtcgggc acatccgcac gcaggccgga
    45001 ccttgttcat caaagctaca aaggtcacat ttgacggcga tcgcgcgaat acccggcacc
    45061 cagtcaagca gcgtactgat acgcgccgga gcaggcggtg ccggtggcgc tttcggggta
    45121 ttggcatttg caggaatatc cagcggacgg ctgccggaaa attcaattgc gccaaacggg
    45181 caggcgatgc cgcacagctt gcagcttacg cacaggcttt cattcaactg cacggcccca
    45241 tcaacgcggg tgatgacgtt aaccgggcag accaccgcgc agggtgcatc ttcacagtgg
    45301 tgacagagct gcggcgcaga ttctttttca ttcagcatca ctctcaggcg cggcattgat
    45361 tgcaggccgt gctggcgatg cgtctctgaa caggctgcct cacaagtgtg gcagccgata
    45421 cagagcgtgg agtcagcaat tacaaaacga ttcaccaggc attcctcagg tgattgtcat
    45481 ttttgacgaa aacatgccga tgaaatgtca ttttcgacac tcatcgacac gcccatcccc
    45541 aaacaggcgt aacgcctgca aaacgggcaa agcctcagct catgctgccg ggctttgtcc
    45601 ctttaccagt tggcttaaat tcaccggcac attgttttca atggcggtgt ataagctgtc
    45661 gtaaacgcca gcgatttttt cgagatagtg ttccagaacc tgttcgcgat cgcggatgtt
    45721 gaccttgcca tcaatcatgc cgtcaatgct cagttgcgcc tgcgcaatcc gctgtttgtc
    45781 ttcgccctct tttgtttcga cgtaatactc gatggtgtgg ctgttaaagc catccgccag
    45841 ggtgacgtaa atacgaaaat gttcaaaaag gacgaaacag agttctttgt ccggcgcgca
    45901 gctcatggcg tgatgcaggc gcgctttcga taacgtgatc ccctgaacca gcgaattgca
    45961 gtagatgtgc cactggtcct gtaggcgacg atgccgctgt gcgatgtaat cggctttctc
    46021 gcttatttcc caaatagtca ttgtcaggtt acccgtttaa cagagatgcc agctttaagc
    46081 attttctgtg ccaattttta atttattgtt attaaaacga tttttaagct aaagatgaat
    46141 ttcgtcgccg tgtcgacgtg tcatttcgac atcatcgaca ttattcaccg cagggataat
    46201 caacactggc acaattattg cttgtagctg gcaatagtta atgggaggcg atatgcacga
    46261 aataaccctc tgccaacggg cactggaatt gatcgaacag caggccgcaa aacacggcgc
    46321 aaaacgcgta actggggtct ggctcaaaat tggcgcattt tcttgtgtcg aaaccagctc
    46381 tcttgccttt tgttttgatc tggtttgccg cggcagcgtg gcggaaggtt gtaaactgca
    46441 cctcgaagaa caagaggtcg aatgctggtg tgaaacatgc caacagtatg tgacgctatt
    46501 gacccagcgc gtccgccgct gtccacagtg tcatggtgac atgctacaga ttgtggcaga
    46561 cgacggttta cagattcggc ggatagaaat agaccaggag tgagcgatgt gtacaacatg
    46621 cggttgcggt gaaggcaacc tgtatatcga gggtgatgaa cataaccctc attccgcgtt
    46681 tcgtagcgcg ccatttgccc cggcggcacg cccgaagatg aaaatcaccg gcattaaagc
    46741 gcctgaattt acccccagcc agactgaaga aggcgacctg cattacggtc atggcgaagc
    46801 gggcactcac gcaccgggca tgagccagcg tcggatgctg gaagtcgaaa ttgacgtgct
    46861 ggacaaaaac aaccgtctgg ctgaacgcaa ccgcgcgcgc tttgctgccc gcaagcaact
    46921 ggtgctcaac ctggtttcca gcccaggttc cggtaaaacc accctgctga cggaaaccct
    46981 aatgcgcctg aaagacagcg ttccgtgcgc ggttattgaa ggtgaccagc aaaccgtgaa
    47041 cgatgccgca cgcattcgcg ctaccggcac accagcgatt caggtgaaca caggtaaagg
    47101 ctgccatctt gacgcacaga tgattgccga cgccgcaccg cgtctgccac tggacgataa
    47161 cggtattctg tttatcgaaa acgttggcaa cctcgtatgc ccggccagct tcgatctcgg
    47221 tgaaaaacac aaagtggcgg tgctttccgt taccgaaggt gaagacaaac cgctgaaata
    47281 tccgcatatg tttgccgccg cctcgctgat gctgctcaac aaagttgacc tgttgccgta
    47341 tctcaacttt gacgttgaga agtgcatcgc ctgcgcccgc gaagtcaatc cagaaattga
    47401 aatcatcctt atttccgcca ccagcggtga agggatggac cagtggctga actggctgga
    47461 gacacagcga tgtgcatagg cgttcccggc cagatccgca ccattgacgg caaccaggca
    47521 aaagtcgacg tctgcggcat tcagcgcgat gtcgatttaa cgttagtcgg cagctgcgat
    47581 gaaaacggtc agccgcgcgt gggccagtgg gtactggtac acgttggctt tgccatgagc
    47641 gtaattactg aagccgaagc acgcgacact ctcgacgcct tacaaaacat gtttgacgtt
    47701 gagccggatg tcggcgcgct gttgtatggc gaggaaaaat aatgcgtttt gttgatgaat
    47761 atcgcgcgcc ggaacaggtg atgcagttaa ttgagcatct gcgcgaacgt gcttcacatc
    47821 tctcttacac cgccgaacgc cctctgcgga ttatggaagt gtgcggtggt catacccacg
    47881 ctatttttaa attcggcctc gaccagttac tgcctgaaaa cgttgagttt atccacggtc
    47941 cgggttgccc ggtgtgcgta ctgccgatgg gtagaatcga cacctgcgtg gagattgcca
    48001 gccatccgga agtcatcttc tgtacctttg gcgatgccat gcgcgtacca gggaaacagg
    48061 gatcgctgtt gcaggcaaaa gcacgcggtg ccgatgtgcg catcgtttac tcgccgatgg
    48121 atgcgttgaa actggcgcag gagaatccaa cccgcaaagt ggtgttcttc ggcttaggtt
    48181 ttgaaaccac catgccgacc accgccatca ctctgcaaca ggcgaaagcg cgtgatgtgc
    48241 agaattttta cttcttctgc cagcacatta cgcttatccc gacgctgcgc agtttgctgg
    48301 aacagccgga taacggtatc gacgcgttcc tcgcgccggg ccacgttagt atggttatcg
    48361 gcactgatgc ctataatttt atcgccagcg attttcatcg tccgctggtg gtcgctggtt
    48421 tcgaacccct tgatctactg caaggcgtgg tgatgctggt ggagcagaaa atagcggccc
    48481 acagcaaggt agagaatcag tatcgtcggg tggtaccgga tgccggtaac ctgctggcgc
    48541 aacaggcgat tgccgatgtg ttctgtgtca acggcgacag cgaatggcgc ggcttaggcg
    48601 tgattgaatc ttctggcgtg cacctgacgc cggattatca acgattcgat gccgaagcac
    48661 atttccgccc ggcaccgcag caggtctgcg atgacccgcg cgcgcgttgt ggtgaggtat
    48721 taacgggcaa atgtaagccg catcaatgcc cgctgtttgg taacacctgt aatcctcaaa
    48781 ccgcatttgg tgcactgatg gtctcctccg aaggagcgtg cgccgcgtgg tatcagtatc
    48841 gtcagcagga gagtgaagcg tgaataatat ccaactcgcc cacggcagcg gtggccaggc
    48901 gatgcagcaa ttaatcaaca gcctgtttat ggaagccttt gccaacccgt ggctggcaga
    48961 gcaggaagac caggcacgtc ttgatctggc gcagctggta gcagaaggcg atcgtctggc
    49021 gttctccacc gacagttacg tcattgaccc gctgttcttt cctggcggta atatcggcaa
    49081 gctggcgatt tgcggcaccg cgaatgacgt tgcggtcagt ggcgctattc cgtgctatct
    49141 ctcctgtggc tttatcctcg aagaaggatt gccgatggag acactgaaag ccgtagtgac
    49201 cagcatggca gaaaccgccc gcacggcagg cattgccatc gttactggcg atactaaagt
    49261 ggtgcagcgc ggcgcggcag ataaactgtt tatcaacacc gctggcatgg gcgcaattcc
    49321 ggcgaatatt cactggggcg cacagacgct aaccgcaggc gatgtattgc tggttagcgg
    49381 tacactcggc gaccacgggg cgactatcct taacctgcgt gaacaactgg ggctggatgg
    49441 tgaactggtc agcgactgcg cggtgctgac gccgcttatt cagacgctgc gtgacattcc
    49501 cggcgtgaaa gcgctgcgtg atgccacccg tggtggtgta aacgcggtgg ttcatgagtt
    49561 cgcggcagcc tgcggttgtg gtattgaaat ttctgaatca gcactgccgg ttaaacctgc
    49621 tgtgcgcggc gtttgcgaac tgctgggact ggacgccctt aactttgcca acgaaggcaa
    49681 actagtaata gctgttgaac gcaacgcagc agagcaagtg ctggcagcgt tacattccca
    49741 tccactgggg aaagacgcgg cgctaattgg tgaagtggtg gaacgtaaag gtgttcgtct
    49801 tgccggtctg tatggtgtga aacgaaccct cgatttacca cacgccgaac cgcttccgcg
    49861 tatatgctaa taaaattcta aatctcctat agttagtcaa tgaccttttg caccgctttg
    49921 cggtgctttc ctggaagaac aaaatgtcat atacaccgat gagtgatctc ggacagcaag
    49981 ggttgttcga catcactcgg acactattgc agcagcccga tctggcctcg ctgtgtgagg
    50041 ctctttcgca actggtaaag cgttctgcgc tcgccgacaa cgcggctatt gtgttgtggc
    50101 aagcgcagac tcaacgtgcg tcttattacg catcgcgtga aaaagacacc cccattaaat
    50161 atgaagacga aactgttctg gcacacggtc cggtacgccg cattttgtcg cgccctgata
    50221 cgctgcattg cagttacgaa gaattttgtg aaacctggcc gcagctggtc gctggtgggc
    50281 tataccccta atttggtcac tattgcctga tgccactggc ggcggaaggg catatttttg
    50341 gtggctgtga atttattcgt tatgacgatc gtccctggag cgaaaaagag ttcaatcgtc
    50401 tgcaaacatt tacgcagatc gtttctgtcg tcaccgaaca aatccagagc cgcgtcgtca
    50461 acaatgtcga ctatgagttg ttatgccggg agcgcgataa cttccgcatc ctggttgcca
    50521 ttaccaacgc ggtgctttcc cgcctggata tggacgaact ggtcagcgaa gtcgccaaag
    50581 aaatccatta ctatttcgac attgacgata tcagtatcgt cttacgcagc caccgtaaaa
    50641 acaaactcaa catctactcc actcactatc ttgataaaca acatcccgcc cacgaacaga
    50701 gcgaagtcga tgaagccgga accctcaccg aacgcgtgtt caaaagtaaa gagatgctgc
    50761 tgatcaatct ccacgagcgg gacgatttag ccccctatga acgcatgttg ttcgacacct
    50821 ggggcaacca gattcaaacc ttgtgcctgt taccgctgat gtctggcgac accatgctgg
    50881 gcgtgctgaa actggcgcaa tgcgaagaga aagtgtttac cactaccaat ctgaatttgc
    50941 tgcgccagat tgccgaacgt gtggcaatcg ctgtcgataa cgccctcgcc tatcaggaaa
    51001 tccatcgtct gaaagaacgg ctggttgatg aaaacctcgc cctgaccgag cagctcaaca
    51061 atgttgatag tgaatttggc gagattattg gccgcagcga agccatgtac agcgtgctta
    51121 aacaagttga aatggtggcg caaagtgaca gtaccgtgct gatcctcggt gaaactggca
    51181 cgggtaaaga gctgattgcc cgcgcgatcc ataatctcag tgggcgtaat aatcgccgca
    51241 tggtcaaaat gaactgcgcg gcgatgcctg ccggattgct ggaaagcgat ctgtttggtc
    51301 atgagcgtgg ggcttttacc ggtgccagcg cccagcgcat tggtcgtttt gaactggcgg
    51361 ataaaagctc cctgttcctc gacgaagtgg gcgatatgcc actggagtta cagccgaagt
    51421 tgctgcgtgt attgcaggaa caggagtttg aacgtctcgg cagcaacaaa atcattcaga
    51481 cggacgtacg tttaattgcc gcgactaacc gcgatctgaa aaaaatggtc gccgaccgag
    51541 agttccgtag cgatctctat taccgcctga acgtattccc gattcacctg ccgccactac
    51601 gcgagcgtcc ggaagatatt ccgctgctgg cgaaagcctt taccttcaaa attgcccgtc
    51661 gtctggggcg caatatcgac agcattccag ccgagacgct gcgcaccttg agcaacatgg
    51721 agtggccggg taacgtacgc gaactggaaa acgtcattga gcgcgcagta ttgctaacac
    51781 gcggcaacgt gctgcagctg tcattgccag atattgcttt accggagcct gaaacgccgc
    51841 ctgccgcaac ggttgtcgct caggagggcg aagatgaata tcagttgatt gttcgcgtgc
    51901 tgaaagaaac caacggcgtg gttgccgggc ctaaaggcgc tgcgcaacgt ctggggctga
    51961 aacgcacgac cctgctgtca cggatgaagc ggctgggaat tgataaatcg gcattgattt
    52021 aactgcaaat tgccggacag atctgcctgt ccggcatact attcacgagg ttttttcgga
    52081 cgatattttt ccggcagttc tggcaccgga cgcttgtcat cgatgagatg acgcacggtt
    52141 aagatcggat gacgccacag cattctcggc ccggcccaac gcataatctg tttcatctct
    52201 tcacgctttg caggctggta acagtgcacc ggacactgct tacaggctgg tttctcttcg
    52261 ccgaacacac atttatccag ccgcttttgc gcgtaaacaa acaacgcctc gtaatgctcc
    52321 ggctccgctg acgcctgcgg gcatttcgct tgataaagat cgatcatttt tttaatcgtc
    52381 agtttttcac gagagatacg cttgccggac atgctgcctc catactctta aggtgtattt
    52441 atattacaac ttaatttgaa aggggactat ggctctgaag gaggaggttt attcaaccga
    52501 cgcaattttg cgtacttgct tcataagcat cacgcaaaag ctgcaaaaca gcatctttcc
    52561 cggaaccagc atcaagaact cgccgttcgc ttcttcccct gaaatgatta actccggtat
    52621 catgtgcgcc ttatgtgatt acaacgaaaa taaaaaccat cacaccccat ttaatatcag
    52681 ggaaccggac ataaccccat gagtgcaata gaaaatttcg acgcccatac gcccatgatg
    52741 cagcagtatc tcaggctgaa agcccagcat cccgagatcc tgctgtttta ccggatgggt
    52801 gatttttatg aactgtttta tgacgacgca aaacgcgcgt cgcaactgct ggatatttca
    52861 ctgaccaaac gcggtgcttc ggcgggagag ccgatcccga tggcggggat tccctaccat
    52921 gcggtggaaa actatctcgc caaactggtg aatcagggag agtccgttgc catctgcgaa
    52981 caaattggcg atccggcgac cagcaaaggt ccggttgagc gcaaagttgt gcgtatcgtt
    53041 acgccaggca ccatcagcga tgaagccctg ttacaggagc gtcaggacaa cctgctggcg
    53101 gctatctggc aggacagcaa aggtttcggc tacgcgacgc tggatatcag ctccgggcgt
    53161 tttcgcctga gcgaaccggc cgaccgcgaa acgatggcgg cagagctgca acgcactaat
    53221 ccggcggaac tgctgtatgc agaagatttc gccgagatgt cgctgattga aggccgtcgc
    53281 ggcctgcgcc gtcgcccgct gtgggagttt gaaatcgaca ccgcgcgcca gcagttgaat
    53341 ctgcaatttg gcacccgcga tctggtcggt tttggcgtcg agaacgcgcc gcgcggactt
    53401 tgtgctgccg gttgtctgtt gcagtatgcg aaagataccc aacgcacgac cctgccgcat
    53461 attcgttcta tcaccatgga acgtgagcag gacagcatca ttatggatgc cgcgacacgt
    53521 cgtaacctgg aaattactca gaacctggcg ggcggtgcgg aaaatacgct ggcttctgtg
    53581 ctcgactgca ccgtcacgcc gatgggtagt cgtatgctga aacgctggct gcatatgcca
    53641 gtgcgcgata cccgcgtgtt gcttgagcgc cagcaaacta ttggcgcatt gcaggatttc
    53701 accgccgggc tacagccggt actgcgtcag gtcggcgacc ttgaacgtat tctggcacgt
    53761 ctggctttac gaactgctcg cccacgcgat ctggcccgta tgcgccacgc tttccagcaa
    53821 ctgccggagc tgcgtgcaca gttagaaact gtcgatagtg caccggtaca ggcgctacgt
    53881 gagaagatgg gcgagtttgc cgagctgcgc gatctgctgg agcgagcaat catcgacaca
    53941 ccgccggtgc tggtacgcga cggtggtgtt atcgcatcgg gctataacga agagctggat
    54001 gagtggcgcg cgctggctga cggcgcgacc gattatctgg agcgtctgga agtccgcgag
    54061 cgtgaacgta ccggcctgga cacgctgaaa gttggcttta atgcggtgca cggctactac
    54121 attcaaatca gccgtgggca aagccatctg gcacccatca actacatgcg tcgccagacg
    54181 ctgaaaaacg ccgagcgcta catcattcca gagctaaaag agtacgaaga taaagttctc
    54241 acctcaaaag gcaaagcact ggcactggaa aaacagcttt atgaagagct gttcgacctg
    54301 ctgttgccgc atctggaagc gttgcaacag agcgcgagcg cgctggcgga actcgacgtg
    54361 ctggttaacc tggcggaacg ggcctatacc ctgaactaca cctgcccgac cttcattgat
    54421 aaaccgggca ttcgcattac cgaaggtcgc catccggtag ttgaacaagt actgaatgag
    54481 ccatttatcg ctaacccgct gaatctgtcg ccgcagcgcc gtatgttgat tattaccggc
    54541 ccgaacatgg gcggtaaaag tacctatatg cgccagaccg cactgattgc gctgatggcc
    54601 tacattggca gctatgtacc ggcacaaaaa gtcgagattg gacctatcga tcgcatcttt
    54661 acccgcgtag gcgcggcaga tgacctggct tccgggcgct caacctttat ggtggagatg
    54721 actgaaaccg ccaatatttt acataacgcc accgaataca gtctggtgtt aatggatgag
    54781 atcgggcgtg gaacgtccac ctacgatggt ctgtcgctgg cgtgggcgtg cgcagaaaat
    54841 ctggcgaata agattaaggc attgacgctg tttgccaccc actatttcga gctgacccag
    54901 ctaccggaga aaatggaaga cgtcgccaac gtgcatctcg atgcgctgga gcacggagac
    54961 accattgcct ttatgcatag cgtgcaggat ggcgcggcga gcaaaagcta cggcctggcg
    55021 gttgcagctc tggcaggcgt gccaaaagag gtgattaagc gcgcacggca aaaactgcgt
    55081 gagctggaaa gcatttcgcc gaacgccgcc gctacgcaag tggatggtac gcaaatgtct
    55141 ttgctgtcag taccggaaga aacctcgcca gcggtcgaag ctctggaaaa cctggacccg
    55201 gattcactga ctccgcgtca ggcgctggaa tggatttatc gcttgaagag cctggtgtaa
    55261 taacaattcc cgatagtctt ttgctatcgg gaatattaac gacaactgac gaataaaata
    55321 aaaaccccct gtataatagg aaagcttatt ttacagggta aaaccatgcc atctacacgc
    55381 tatcaaaaaa ttaatgccca tcactatcgc catatatggg tcgttggtga tattcatggt
    55441 gaatatcaat tattacaatc ccgcttacat caactctctt tttgccctga aaccgactta
    55501 cttatttctg tcggcgataa tattgatcgt gggccggaga gtcttgacgt cctgcgcctg
    55561 ctaaaccagc cctggtttat ctccgttaaa ggcaaccacg aagcaatgga gctggatgcg
    55621 ttcgcgaccg gcgatggcaa tatgtggctt gccagcggtg gtgactggtt tttcgattta
    55681 aatgattcag agcaacaaga ggcaatagat ctgttgctga aattccatca ccttccacat
    55741 attattgaaa tcactaacga cataataaaa tatgtcatcg cacatgcaga ttatccgggt
    55801 gatgaatatc tctttggcaa agaaatagcg gagagcgaat tactctggcc tgtttatcgt
    55861 gtgcagaaat cgcttaatgg cgagttacaa caaataaacg gcgctgatta ttttatattt
    55921 ggacatatga tgtttgataa cattcagacg ttcgctaacc agatttatat tgataccgga
    55981 tcgccgaaaa gtgggcggct gtcattttat aaaataaggt agtctcatgc ttcttctgtg
    56041 aagcatgagt aaccagatgt tattgcaggc cattattcat ttttcgctac cagcaaagag
    56101 agatcctgct tcgccagcgc gcgactggca ctctccggca aaccgtcgtc tgtaataatc
    56161 tgatcaaact cgcttaatgg taacgccagc catgtcgcca cctgaccata tttcgtcgca
    56221 tcacagacca aaactcgctg gcggctggca ctagcaatcg cccgtttcac cgtgacttta
    56281 tcttccgctg gcgtagaaat cccccgcaca ctccatgacg atgcagaaat aaaagcctga
    56341 tcaatcatca ggctgcgcag catggtcgca gcggcttccc cgacacagga acggttttcc
    56401 cgacacactg caccgccagt gtgaataatt gtgcaattac tgttgtcgag caagtagttc
    56461 gcaataacga aatcgtttgt gaccacagtc agtgactcca tgtgaatcag atgctgtgct
    56521 atcgctaacg tggtcgttcc cgcatccaga tagatacaac ttcccggctg aacaagactt
    56581 gccgccagct tgccaatagc cgctttttgc gtcattgcta gcgcagtttt tacctgatga
    56641 gaaggttcat gcgccacgcg tcccggagac tggacgcctc cggacaccag cacaacggct
    56701 ccctgctgct ccagtttttg taaatcccga cgaatggtca tatgtgatac attcattctg
    56761 tccgttagtt cagcaatact gacaatgcct ttttcagcta ccatctcaag gatgatttgg
    56821 cgacgctcta cgggtatcaa cttttgctcc ttcctttgtc ctgctgacat tctacgctat
    56881 ttgcctgcga aacgtgcgcg gcgcaactaa cgcttagttc acataaaata acacacaatg
    56941 ttaatttatg tgaataggct caccatatcg ttatcttcca gcgcttatat tcacaatatc
    57001 aaacaaaata tcacttaaat taacaaggag agcagatgaa aacgggatct gagtttcatg
    57061 tcggtatcgt tggcttaggg tcaatgggaa tgggagcagc actgtcatgt gtccgcgcag
    57121 gtctttctac ctggggcgca gacctgaaca gcaatgcctg cgctacgttg aaagaggcag
    57181 gtgcttgcgg ggtttctgat aacgccgcga cgtttgccga aaaactggac gctctgctgg
    57241 tgctggtggt caatgcggcc caggttaaac aggtgctgtt tggtgaaaca ggcgttgcac
    57301 aacatctgaa acccggtacg gcagtaatgg tttcttccac tatcgctagt gctgatgcgc
    57361 aagaaattgc taccgctctg gctggattcg atctggaaat gctggatgcg ccagtttctg
    57421 gtgatgcagt aaaagccgct aacggtgaaa tgactgtcat ggcctccggt agcgatattg
    57481 cctttgaacg actggcaccc gtgctggaag ccgttgccgg aaaagtttat cgcataggtg
    57541 cagaaccggg actaggttcg accgtaaaaa ttattcacca gttgttagcg ggcgtacata
    57601 ttgctgccgg agccgaagcg atggcacttg cagcccgtgc ggggatcccg ctggatgtga
    57661 tgtatgacgt cgtgaccaat gccgccggaa attcctggat gttcgaaaac cggatgcgtc
    57721 atgtggtgga tggcgattac accccgcatt cagccgtcga tatttttgtt aaggatcttg
    57781 gtctggttgc cgatacagcc aaagccctgc acttcccgct gccattggcc tcaacagcat
    57841 tgaatatgtt caccagcgcc agtaacgcag gttacgggaa agaagacgat agcgcagtta
    57901 tcaagatttt ctctggcatc actctaccgg gagcgaaatc atgatcaaga ttggcgttat
    57961 cgccgatgat tttaccggcg cgacggatat cgccagtttt ctggtggaaa acggtctacc
    58021 aacggtacaa attaacggtg ttccaacagg taaaatgccg gaagcaatcg acgcactggt
    58081 gatcagcctg aaaacgcgct cctgtccggt ggttgaagcc acacagcaat cgctggcggc
    58141 tctgagctgg ttgcaacagc aaggttgcaa acagatctat ttcaaatact gctctacttt
    58201 cgacagtacg gcgaaaggta atatcggccc ggttaccgat gcattaatgg atgctctcga
    58261 cacgccgttt acggtcttct ctccggccct gtcggtcaac ggacgtacgg tttatcaggg
    58321 gtatttgttc gtaatgaatc aactgctggc cgaatccggg atgcgccatc acccggtaaa
    58381 tcccatgacc gacagctatc ttccccgtct ggttgaagcg caatccacag ggcgctgcgg
    58441 cgtcgtttcg gcacatgttt tcgaacaagg tgtggatgcc gttcgtcaag agctggctcg
    58501 cttacagcaa gagggctacc gctacgcggt gcttgatgcg ctgaccgaac accatctgga
    58561 aattcaggga gaagccttgc gcgatgcccc actggtaacg ggcggttctg gtctggcgat
    58621 tggcctggcc cggcagtggg cgcaagaaaa cggtaaccag gctcgcgaag cagggcatcc
    58681 gctcgctggg cgcggcgtag tgctctccgg ttcatgctct caaatgacca accgccaggt
    58741 ggcacattac cgtcaaattg caccagcccg tgaagttgat gtggcacgct gcctctcaac
    58801 cgaaactctg gccgcttatg cacacgaact ggcagagtgg gttctgggcc aggaaagtgt
    58861 acttgctcca ctggtttttg ccaccgccag cactgacgca ttggcagcaa ttcaacagca
    58921 atacggtgca caaaaagcca gtcaggcagt agaaactctg ttttctcaac tagcggcgcg
    58981 gttagcagcg gaaggcgtga cacgctttat tgtcgcaggc ggtgagacct ccggcgtagt
    59041 cacacagagc ctgggcataa aagggtttca tattggccca accatttccc ccggcgtgcc
    59101 gtgggtaaac gcactggata agcctgtctc actcgccctt aaatctggca acttcggtga
    59161 tgaagccttt ttttcacgag cccaaagaga gtttttatca tgagcgattt cgcaaaagta
    59221 gagcagtctt tgcgagagga gatgacgcgg attgccagtt cattctttca gcgcggctat
    59281 gcaaccggtt cggctggcaa tctgtcgctg cttttacctg acgggaattt actggcgaca
    59341 ccgacaggtt catgcctggg caatctcgat ccgcagcggc tttccaaagt cgccgcggat
    59401 ggcgaatggt taagtggtga caaaccctcg aaagaggtgc tctttcatct ggcgctgtat
    59461 cgcaacaatc cgcgctgtaa agcggtggtg catttgcaca gcacatggtc gacggtgctt
    59521 tcctgcctgc aagggctgga cagcagcaac gttattcgtc cgttcacacc atacgtggtg
    59581 atgcggatgg gaaatgtccc gctggtgccc tattaccgac cgggcgataa acgcatcgca
    59641 caggatctgg cggaactggc agcagacaat caggcttttt tactggcaaa tcatggccca
    59701 gtggtttgcg gtgaaagcct gcaagaagcc gccaacaata tggaagagct ggaggaaacg
    59761 gcaaagctga tttttattct cggtgaccgc ccgatccgtt atctgaccgc aggtgaaatt
    59821 gcggaattaa ggagttaatg caatgcctcg ttttgcagct aatttatcca tgatgttcac
    59881 cgaagtgcct tttattgaac gcttcgccgc agcgcgaaaa gccgggttcg atgctgtgga
    59941 atttctgttt ccctatgact actccaccct gcaaatccaa aagcaactgg agcaaaacca
    60001 tctgacactg gcgctgttca ataccgctcc cggagatatt aatgcggggg aatggggatt
    60061 atccgccctt cccggacgtg agcacgaagc acacgcagac atcgacctgg cactggaata
    60121 tgcattggcg ctcaattgtg aacaagtcca tgtgatggca ggcgtcgtgc ccgctggcga
    60181 agatgctgaa cggtaccggg cagtatttat cgataatctc cgctacgctg ccgaccgctt
    60241 tgcgccacac ggcaagcgaa tattagttga agcactcagc cccgacgtta agcctcatta
    60301 tctcttctcc agccagtatc aggcactggc tattgttgaa gaggttgcgc gagataacgt
    60361 gtttattcaa ctagatactt ttcatgcgca aaaagtagat ggcaacctga cgcatttaat
    60421 ccgcgactac gctgggaaat atgcgcacgt acaaattgcc ggactacctg atcgacatga
    60481 ccggacgatg gagaaatcaa ctatccgtgg ctgttccgcc tgttcgatga ggtgggatat
    60541 cagggctgga tcggttgtga atataaacct cgtggcctca ccgaagaagg gcttggctgg
    60601 tttgacgcct ggcgctaatt cgttaaatcc ctaattacaa cgtacccata catcccccca
    60661 tctacggatg gggtaggtta actataactt tcagacaggg tttcccatgt ccacaattac
    60721 attgttatgc attgcgttag ctggcgtaat catgctgttg ctgctggtca tcaaggcaaa
    60781 ggtacaacca ttcgttgctc tgctcctcgt cagcctgtta gtcgcacttg cggcgggtat
    60841 accggcgggc gaagtgggta aagtgatgat cgccgggatg ggcggtgtgc ttggttccgt
    60901 cactattatt attggtctgg gcgctatgct ggggcgtatg atcgaacact ctggtggtgc
    60961 agagtcactg gctaattatt tcagtcgcaa gttaggtgac aaacgaacta tcgctgcgct
    61021 gactctggca gcgttcttcc tcggtattcc cgtcttcttt gatgtcagct ttattattct
    61081 tgcgccaatc atttacggtt ttgccaaggt tgccaaaata tcgccactca aatttggcct
    61141 gcctgtcgct ggaatcatgc taactgttca tgtggcagta ccgccgcatc caggccctgt
    61201 cgccacagcg gggttactcc acgcagacat cggctggcta accatcatcg gtattgcgat
    61261 ttctattccc gtagggattg ttggctactt tgcagcgaaa ataatcaata agcgccaata
    61321 tgcgatgtca gtagaagtac tggaacagat gcaactggct ccggccagtg aggaaggcgc
    61381 gacaaaatta agcgataaaa taaatccacc gggcgtcgcg ctggtcacct cgctaattgt
    61441 tattcctatc gcgattatca tgacgggtac ggtttccgca acactgatgc cgccttcgca
    61501 tcccctgctt ggtacgctac agctgatcgg ctcaccaatg gtagctctaa tgattgcgct
    61561 ggtgctggca ttctggttat tggctttacg tcgcggctgg agcttacaac acaccagcga
    61621 cattatgggc tcagcccttc ccactgcggc ggtagtgatt ttggttactg gtgctggagg
    61681 ggtatttggc aaagtgctgg tggaatcggg cgtcggcaaa gcccttgcca atatgctaca
    61741 aatgattgac ctgcctctgt taccagccgc atttattatt tcactggctc tgcgtgcatc
    61801 gcaggggtca gcaaccgtag caatcctgac aaccggcggg ttactctcag aagcggtgat
    61861 gggattgaat ccgattcagt gcgtattggt gacgctggca gcctgctttg gtgggcttgg
    61921 cgcgtcacat attaatgact cagggttctg gattgtgacc aaatatctgg ggttgtcggt
    61981 agcagacggt ctgaaaacct ggacagtgtt aacgaccatt ctcggtttta ccggattctt
    62041 aattacctgg tgcgtatggg cggtaatttg accttaataa aaaggtccga tgggcatcgg
    62101 accttttatt gtgcacagaa aaggccagcc tcgcttgaga ctggcctttc tgacagatgc
    62161 ttacttactc gcggaacagc gcttcgatat tcagcccctg cgtttgcagg atttcacgca
    62221 aacggcgcag gccttcaacc tgaatctggc gaacacgttc acgggtgagg ccaatttcac
    62281 gacctacatc ttccagtgtt gccgcttcgt accccagcaa accgaatcga cgtgccagca
    62341 cttcacgctg tttggcgttc agctcgaaca gccatttgac gatgctctgc ttcatatcgt
    62401 catcttgcgt ggtatcttcc ggaccgttct ctttttcatc ggccaggatg tccagcaacg
    62461 ctttttcgga atcaccaccc agcggggtgt ctaccgaggt aatgcgctcg ttaagacgaa
    62521 gcatacggct gacgtcatca actggcttat ccagttgctc tgcgatctct tccgcacttg
    62581 gttcatggtc cagcttatgg gacaactcac gtgcggttcg caggtaaacg ttcagctcct
    62641 ttacgatgtg aatcggcaaa cgaatagtac gggtttggtt cataatcgcc cgttcaatcg
    62701 tctggcgaat ccaccaggtt gcgtatgttg agaagcggaa accacgttcc gggtcaaact
    62761 tctctaccgc gcggatcagc cccaggttgc cctcttcgat aaggtccagc aacgccagac
    62821 cacgattgcc ataacggcgg gcaattttta ccaccagacg caagttactc tcgatcatcc
    62881 ggcggcgaga ggcgacatct ccacgcagtg cgcgacgcgc aaaataaact tcttcttcgg
    62941 ccgttaacag tggtgaataa ccaatctcac caaggtaaag ctgagtcgcg tccaacactc
    63001 gctgtgtggc tccctgcgat aacagttcct cttcggccaa atcgttatca ctgggttcct
    63061 cttctactaa ggccttttcg tcaaaaacct caactccgtt ctcatcaaat tccgcatctt
    63121 catttaaatc atgaactttc agcgtattct gactcataag gtggctccta cccgtgatcc
    63181 cttgacggaa cattcaagca aaagcctggt tccgccgatt tatcgctgcg gcaaataacg
    63241 cagcgggttt acggatttcc ccttgtaacg aatttcaaaa tgcaagcgtg ttgaactggt
    63301 tccggtgcta cccatggttg ctattttttg ccccgccttc acttcttgtt gttcccggac
    63361 cagcattgtg tcgttatggg cgtaggcact caggtaatca tcattatgtt tgatgataat
    63421 cagattaccg tagccgcgca gcgcgttacc ggcataaaca acgcggccat ctgcggtcgc
    63481 gataattgcc tgtcctttgc tgcctgcgat atcaatcccc ttgttgcccc cctcagaagc
    63541 gccaaaggtt tcgatcactt tgccctcagt cggccagcgc caggtggaga taggcgtact
    63601 ggttgatgta ctgctgacag ttggctcggt tgtgcttgct gttggtaccg ttacaggcgc
    63661 tgtgaccgtg gtcgcagctg gcttgttgtt cggcaacatt ttgttagcac tctgttcacc
    63721 cgaagattca gaatacgtaa ttgtcggttg cgacgcaaca gcaacggtgg aattttgtgc
    63781 aggcttgatc acaactcctt gctctgctgc gtcggcctgg gtaatggcat ttccgccagt
    63841 gattggcgta ccggaagcat tacccacctg caaggtctga ccaacgttca gcgcgtatgg
    63901 tgcctgaata ttgttgcgct gagcaaggtc acggaaatcg ttgccagtaa tccaggcgat
    63961 atagaaaagt gtgtcgcctt ttttcacggt ataggtactg ccgctataac tgcctttcgg
    64021 aatgttccca tactgacggt tatagacgat gcgtccgttt tccatctgta ccggctgctg
    64081 agctactggc tgcactggct ggatttgcgg ttgctgggct gtagacgtcg tccccatttt
    64141 cggcggcggc gtaatcaaca taccagaatt ggtatttgca ggcgcattgc cattaacgga
    64201 gctgaccggt gccggtggat ttgaagtgtc agaacagcct gccagccata gcgaaaccag
    64261 tgacaaagcc gcaatgcggc gaacggtgaa ttttgggctt cccgcgctca tttatccccc
    64321 aggaaaaatt ggttaataac cagtgacata attaccgtgc aaggcaccct actgaacact
    64381 ggaaaagatg ttcacgatac gctgacctgc ggcaaaataa ccaggaaaaa tccaggtatt
    64441 tcctcacgtt ttaagccagc tcccccttca ctaaagggac aaagcgcacg gcctccacgg
    64501 tatcgataat aaattcgcct ccccgacgac gcacccgttt caaatactgg tgctcctccc
    64561 ctacgggtaa gacgagaatc ccgccttcgt ccagctgcgt cattagcgca gttggaattt
    64621 ccggcggtgc cgccgtaaca atgatagcgt caaacggcgc gcgtgcctgc caaccttgcc
    64681 atccatcgcc atgacgggtt gaaacattat gtaaatcaag atttttcagg cggcgacgcg
    64741 cctgccactg caaaccttta atccgttcga ccgagcaaac atgctggaca agatgcgcca
    64801 ggattgccgt ttgatatccc gaaccggtgc caatttccag cacccgcgac tgcggcgtca
    64861 gctcgagtaa ttcggtcatt cgcgccacca tatacggctg cgaaattgtc tgcccctgac
    64921 ctatcggcaa ggcgatattg tcccaggctt tttgttcaaa cgcttcatca acgaattttt
    64981 cacgcggcac ggcagcaagt gcattcagca cctgctcatc ctgaatacct tgcgcacgta
    65041 attgatccag aagtgcttgt acgcgtctgc ttaccattgc gtgccaactc ccacgctgtt
    65101 taaccagtct gaaaccacat cttgcgcgct atgcgcagtt aaatccacat gcagcggcgt
    65161 gatggagaca tagccctcat ctaccgcagc aaaatcggtc cccggaccgg catcacattt
    65221 accgcccggc gggccaatcc agtacagcgt attgccgcgc ggatcttgct gcgggatcac
    65281 ctgatctgcc ggatgtcgtg taccgcagcg cgtcacgcga atacctttga tttgatccaa
    65341 gggtaaatcc ggaacgttaa tattaagaat acgtccggtg cgcagcggct ctttacacag
    65401 tgcgcgcaaa attgaacagg ttaccgccgc ggcagtgtcg taatgtttat gcccgtcaag
    65461 cgagacggcc agcgccggaa aacctaaatg acggccttcc atcgcggcgg ctaccgtacc
    65521 ggaataaata acgtcatccc ccagattcgg cccggcgtta atgccggaca caacaatgtc
    65581 cgggcgcgga cgcatcagag cattcacgcc aagatagacg caatcggtcg gggttcccat
    65641 ttgcacagca atatcaccat tttcaaaggt aaacgtgcgc agggaggatt ccagtgtcag
    65701 agaatttgaa gcgccgctgc ggttacgatc gggggcgacc acctgaacgt cagcaaactc
    65761 acgcaaggct ttcgccagcg tttgtatacc gggtgcatgt accccgtcat cattactcag
    65821 caatatgcgc ataatcacct gttgtgttga taagttccct gacaacgctg gttgcaaaac
    65881 tacccgccgg aagccagaaa cggatctcta cggtgacgtc atcccaccaa ttccagctta
    65941 attgttgcgg atacagcagc atcgctctgc gcgcggcttc aactttttcg cgcaccagta
    66001 aagcttgtaa ttcagtttct gcggcgacag ctgcttgttc gaatgccagc gctttacgct
    66061 gagttcccca ttcgccactg cccggcaatg cggcggttat catcagctct ttatcgttga
    66121 cgcgatgctg taattccgcc agttcttcgg tggttgctac aaaccagcta ccacgtccgg
    66181 ctaattgtag cgcatcgccg tcaacaactt gattaacgtc tgcttttttg aggcgctcag
    66241 caacaatctg attaaacaac gcactgcggg ctgccgacaa ccaaaaactc cgtttattgc
    66301 gatcgcgcac cggagtattg gtttgcgccc agcgcagcgc gccctgcaag ttgctaccgc
    66361 caatcccaaa acgttgggca ccgaagtagt tcggtacacc ttttacgcaa atatcgatca
    66421 gacgttgttc aacgtcatcg cgattgctca cttcgcgtag aaccagggta aaggcgttac
    66481 ctttcagcgc gcctaaacgc agcttgcgct tgtgccgcgc atactccagc acctggcagc
    66541 cttccagttg aaaggcgctc agatcgggca tttccttgcc tggcacgcga gcgcataacc
    66601 actgttccgt aacagcatgt ttgtcttttt gcccagcgaa gctgacttca cgggcatgaa
    66661 ttttcaggaa tttcgccagt gcatccgcca caaaacgggt attgcagccg tttttgagga
    66721 ttctaaccag aatatgctca ccttcaccat caggctcaaa gcccaaatct tccaccacca
    66781 caaagtcttc cggattggct ttcagcagcc cggtgccttg cggtttaccg tggaggtaag
    66841 tgagattatc aaactcaatc attttgttgc cttaatgagt agcgccaccg cttcacaggc
    66901 aatcccttcc ccacgtccgg taaatccaag tttttccgta gtagtggctt tcacgttaac
    66961 atcatccata tggcagccga gatcttcggc aataaacacg cgcatttgtg gaatgtgcgg
    67021 caacatcttc ggtgcctgag cgatgatagt gacatcgacg ttgcccaggg tataaccctt
    67081 cgcctgaata cgacgccagg cttcgcgtag cagctcgcgg ctgtcggcac ctttaaatgc
    67141 cggatcggta tccgggaaca gcttgccgat atcccccagc gccgccgcgc caagcaatgc
    67201 atcggtcaac gcatggagcg ccacgtcgcc atcagaatgc gccagcaatc ctttttcgta
    67261 aggaatgcgt acgccaccaa tgataattgg gccttcaccg ccaaaggcat gtacgtcaaa
    67321 accgtgtcca attcgcatta tgtattctcc tgatggatgg ttcgggtgag gtaaaactcg
    67381 gccagtgcca aatcttccgg gcgcgtgact ttaatgttat ccgcacggcc ttcgaccaac
    67441 tgaggatgga atccgcaata ttccagcgcc gaggcttcgt cggtaatagt cgcgccttca
    67501 tttagagcgc gcgtcagaca gtcatgtaac agctcacgag ggaaaaattg cggcgtcagc
    67561 gcgtgccata agccgttgcg atcaacggta tgagcaatgg catttttgcc cggttcggca
    67621 cgtttcatcg tatcgcgcac tggtgcggct aggatccctc ccgtgcggct ggtttcgctc
    67681 aacgccaaca atcgcgcgag gtcatcctga tgcagacaag gacgagcggc gtcatgcacc
    67741 aatacccact gcgcgtcgcc agcggctttc aaacctgcca gcacggaatc ggcacgctca
    67801 tcaccgccat ctacaacggt gatttgcgga tgattcgcca gaggaagttg tgcaaaacgg
    67861 ctatcgccag gacttatggc aatgacgaca cgtttcaccc ggggatgcgc cagcagcgca
    67921 tgcaccgagt gttcaagaat ggtttgatta ccgattgaga gatattgctt aggacattcc
    67981 gtttgcattc gacggccaaa tccggccgcc ggaaccacgg cgcaaacatc caaatgagtg
    68041 gttgccatgt taattcccgg gctgatttat cgattgtttt gccccgcaga ctgtgcgcgc
    68101 ttcgacgcgt caggcaccag acgataaaaa gtttcgcccg gcctggtcat gctgagttca
    68161 ttacgcgcac gctcttcgag cgcctcctgg ccgccattga gatcgtcaat ttcggcaaaa
    68221 agttgatcgt ttcgcgcttt aagtttcgcg tttgtagctt gctgtgccgc cacatcatca
    68281 ttgacgcggg tatagtcatg tataccgttc ttaccgaacc acagcgaata ctgtagccag
    68341 accagaatag ccagcaacag cagcgttagt ttacccatcc tgccccctga aaaacggcat
    68401 catcatccca tgcatccgaa gacgactcta catcctctgt tggggatacc gcgacaacgc
    68461 gggcaaatgt accacatttg tccattgtta cgtataccta gggcgtgcag aacataatct
    68521 cattattagt tacggtttga attatgaaca gaggagacaa gaaagtacaa attagcccag
    68581 tagccacata aacagtgcgc caaacataat gcctactgtc accagggtga aaacaatact
    68641 gtagcgtagc tttccgtcca tcaatgaatg cagcgcaatc cccaccacta ccgcgacagg
    68701 catcagtgcc agaaagaaag gccaggtgta gataaagaag aacagcgtgt tagagccata
    68761 aatcaacatc ggcatcgcca gcgcaaataa ccaggagata aaaccgacca cggcaccagg
    68821 cagtgaccat gtggtttctt catcctcagt aaggctgtcg ttatttgtta gtgtaatgtt
    68881 atggctatta cgcatatttg atcctgttac tttgacgaac cgggcatgga aacccggtgg
    68941 tgtctcagga tctgataata tcgttctgtc tcaacagatc taataattgc tgtaccaaat
    69001 ttgttactaa ttgttcacca ttgagatgaa tttctgccga ttcaggcgct tcgtaaacgg
    69061 aatctattcc cgtaaagttg cgcagttcac cggcacgcgc tttcttatat aagcctttgg
    69121 gatcgcgggc ttcgcaaatc gccagcggcg tatcgacaaa cacttcgata aagcgccctt
    69181 ctcctacgcg ttcgcgaacc atctggcgtt cggcgcggtg tggcgagata aatgcggtca
    69241 gcaccaccag tccggcttca accatcaaat tcgccacttc accgacgcga cggatattct
    69301 ctttacgatc ggcatcgcta aaaccgagat cgctgcataa tccgtggcga acattgtcgc
    69361 catccagcag atacgtactg acgccgagtt tatgtaacgc ctcctccagc gccccggcga
    69421 ccgttgattt accggaccca gagaggccgg taaaccacag cactacacca cgatgaccgt
    69481 ggtgtagctc gcgttgttgc acagtgaccg gatggctatg ccagacgacg ttttcgtcat
    69541 gcagcgccat tatttatccc ccagcaaatc gcgcgcgccc cagtgcggaa agtggcgacg
    69601 aaccagggca ttcaattcca gttcgaatgc actgaattca gatgacgcaa cggcggcctc
    69661 gtttttcggc tcatgcacca taccggcacc aacagtcacg ttgctcaggc gatcgataaa
    69721 aatcagccca cccgtcaccg gattttgttg ataacgatct aacaccagcg gctcgtcaaa
    69781 agtgagatcc acgaggccga tcccattcag tggcaggttt tcaacttcac gctgggtaag
    69841 gttattaata tcaaccggat agcgaatgcc atcaacacgc gcgcgcgtct tcttaccggc
    69901 aattttgatg tcgtaactct ggcccgggga aagcggctgt tccgccatcc ataccacatc
    69961 caccgacgcg ctcggcaccg ccggtaacgc ttcgtctgcc gccagcagca gatcgccacg
    70021 gctgatgtcg atctcatccg tcagcaccag ggtgatcgct tctccggcaa aggcttcttc
    70081 gcgatcacca tcaaaagtca cgatccgcgc gacgtttgat tccacaccag agggcagcgc
    70141 tttgacacgt tgcccgactt ccacgcgacc ggatgccagc gttccggcgt agccacgaaa
    70201 atcgagattc gggcgattaa cgtactgcac cgggaagcgc attggctggg catccaccac
    70261 tcgctggatc tccacggttt ccagcacttc gagcagtgtc ggaccgctgt accacgacat
    70321 actttcactt tgcgatgcca cgttgtcgcc ttccagtgca gagagcggca caaagcggat
    70381 atccagatta cccggcagct gcccggcaaa ggtcaaataa tcttcacgaa tatgggtgaa
    70441 cgtctcttca ctgtaatcca ccagatccat tttgttgatc gccacgacca gatgtttgat
    70501 ccccaacagt gtggagataa aactgtgacg acgggtttga tcgagcacgc ctttacgggc
    70561 atcgatcagt aagatcgcca gttcacatgt cgatgcgcca gttgccatat tgcgagtgta
    70621 ctgctcgtgc cctggggtgt cggcgataat aaatttacgc ttctcggtag agaaataacg
    70681 gtaagccacg tcaatggtga tgccctgttc gcgctcagct tgcaggccgt ccaccagcag
    70741 tgccagatcc agcttttcgc cctgggtgcc gtgacgctta ctgtcgttat gcagcgatga
    70801 gagctgatct tcgtagattt ggcgggtatc gtgcagcagg cgaccaatca gggtactttt
    70861 gccgtcatcg acgctaccac aggtcagaaa acgcagcagg cttttatgtt gttgcgcaat
    70921 catccaggct tcgacgccgc cttcattggc gatttgttgt gcaagtgcgg tgttcatctt
    70981 aaaaataccc ctgacgtttt ttcagctcca tagaccccgc ctggtcgcgg tcaatcacgc
    71041 ggccctgacg ttcactggtg gtagaaacca gcatctcttc gatgatctcc ggcagtgttt
    71101 gtgcatttga ctccaccgca ccggtcagcg gccagcagcc cagcgtacgg aaacgcacca
    71161 tccgtttttt aatcacttcg cccggttgca ggtcgatacg gttgtcatca atcatcatca
    71221 acataccgtc gcgttccaga accggacgtt ccgcagcgag atacagtggc acaatgtcga
    71281 tattttccag ccagatgtat tgccagatat cctgctcggt ccagttagag agcgggaaga
    71341 cgcggatgct ttcacctttg ttaatttgcc cgttgtagtt gtgccacagc tccgggcgct
    71401 gatttttcgg atcccagcga tggaagcggt cacggaaaga gtagattcgc tctttagcgc
    71461 gggatttctc ttcatcacgg cgcgcaccac cgaaggcggc atcaaaaccg tatttgttca
    71521 gcgcctgttt caggccttca gttttcataa tatcggtatg tttcgcgctg ccgtgtacga
    71581 atggattaat ccccatcgcc acgccttccg ggtttttatg caccagcagc tcgcagccgt
    71641 aggctttagc agtacgatcg cggaactcat acatctcgcg gaatttccag ccggtatcga
    71701 catgcagcaa cgggaaaggc agcgtacctg gataaaacgc cttgcgcgcc agatgcagca
    71761 tgacgctgga atctttaccg atagagtaga gcatcaccgg atttgagaat tctgccgcca
    71821 cctcgcgaat aatgtggatg ctttccgcct ccagttgccg caggtgagta agtcgttttt
    71881 gagccataac cgttcctttg caataccgct attttcttgc catcagatgt ttcgactata
    71941 gggagcgtaa gagaacgaat gaaattacca attagaatga gtagttcctt aacggaataa
    72001 cgatttggca aagctaatat caaaaagtgc ttaaggcacc ggatttcggg cgtttaggaa
    72061 gatttgaaat tgttttagcg cagcggcagt ttcatactat ggcggtaaaa aaatttgcat
    72121 ggtatttaag gactcactat gttttccgca ttgcgccacc gtaccgctgc cctggcgctc
    72181 ggcgtatgct ttattctccc cgtacacgcc tcgtcaccta aacctggcga ttttgctaat
    72241 actcaggcac gacatattgc tactttcttt ccgggacgca tgaccggaac tcctgcagaa
    72301 atgttatctg ccgattatat tcgccaacag tttcagcaaa tgggttatcg cagtgatatt
    72361 cggacattta atagtcggta tatttatacc gcccgcgata atcgtaagag ctggcataac
    72421 gtgacgggaa gtacggtgat tgccgctcat gaaggaaaag cgccgcagca gatcatcatt
    72481 atggcgcatc tggatactta cgccccgctg agcgatgctg acgccgatgc caatctcggc
    72541 gggctgacgt tacaaggaat ggatgataac gccgcaggtt taggtgtcat gctggaattg
    72601 gcagaacgcc tgaaaaatac gcctaccgag tatggtattc gatttgtggc gaccagcggc
    72661 gaagaggaag ggaaattagg cgctgagaat ttactcaagc ggatgagtga caccgaaaag
    72721 aaaaatacgc tgctggtgat taatctcgat aacttaattg ttggcgataa attgtatttc
    72781 aacagcggtg taaaaaccac tgaagcagta aggaaattaa cgcgcgacag ggcgctggca
    72841 attgcgcgca gtcacggaat agccgcaacg accaatccgg ggttgaataa aaattatccg
    72901 aaaggcactg ggtgttgtaa tgacgcagaa atattcgaca aagcgggcat tgctgtactt
    72961 tcggtggaag cgactaactg gaatcttggg aataaggatg gttatcagca acgcgcaaaa
    73021 acagccgcat tccctgcggg aaatagctgg catgacgtaa gactggataa tcaacagcat
    73081 attgataagg cacttcctgg aagaatagaa cgtcgctgcc gtgacgttat gcggataatg
    73141 ctaccgttgg tgaaggagct ggcgaaggcg tcttgatggg tttgaaaatg ggagctggga
    73201 gttctaccgc aggggcgggg aattctaagt gatatccatt atcgcatcca gtgcgcccgg
    73261 tttatccccg ctggcgcggg gaacacacta agcatacata actgttttta aacaaattta
    73321 ttccgcatca acaatctacc aactaaatta aaacatttcc ttatttttaa agaacggata
    73381 acttattgat tatcaacagg aagaaaagaa accaaacgta acccatccaa atccaccgga
    73441 atacgtctgt tttctcccca ggtctgaaat tcaaaacccg actcggtatt ggtcgcccag
    73501 gccatcacca catttccgca accagccagt tgggtaattt gctgccagat catctcccga
    73561 atacgttttg atgtatcacc aacatacaca ccggcacgca cttccagtag ccagattgcg
    73621 agccgtccac gtaagcgtgg cgggacattt tctgtaacaa ccacgaccat gctcatcagc
    73681 cgcgtcctcg atgaccgcta tcgcccaacg tttcaggttc ggggatggct ggcggtaaca
    73741 tatccggcgc aggttgtggc ggttcaattt cacctgcggc aaggacttcc tcaattaacg
    73801 gtattaattt gcccgttaac ttagtgctac ggaaaatatc acgacaggct aatctgactt
    73861 ctttatcagg ttctgcgggt tgcctcgccg ctatttcaaa tgcctttggt acaaccgaat
    73921 caaatttaat gatatcggct atgtcataaa caaatgaaag cggtttgcca ctatggataa
    73981 acccaatagc gggcgcatat cccgcggcta atactgccgc ttcagatata ccgtacagac
    74041 atgatgtagc agcactgatg cagcgattca caacatcgcc tttttcccag tctttaggat
    74101 cgtatttgcg accattccat ttcacaccat attgtttcgc cagtaatgca tacgtctggc
    74161 gaacgcgaga tccctcaatt ccacgtagct gctccactga acggcgagct ggcggtggct
    74221 cacgaaaacg taattcatac attttgcgca ccaccttcag acgtagatct tccgttaaag
    74281 ccagctttgc ctggtagagt aacttatcag cacgcgcccc accgggttgt ccggaagagt
    74341 agacgcgaac acccgcttca ccgacccaga cctgtagcgt ccccaccgtg gcggccagat
    74401 gcaccgccgc gtgggaaact ctcgttcccg gttcgagcat aatgcaggcg accgatccca
    74461 ccggaatgtg cgtgcggatc ccggttttat cgatcagcac gaaagcgccg tccagtacgt
    74521 cgatttgccc gtactggagg aagatcatag aggtgcgatc ttttaacggg atcgggctca
    74581 gcggtacaaa cgtcacgcct ctgctccggg tttgatcagc atcagaccac agccgaacgc
    74641 gcggcttttg ccatacccct gactgagacg ttgcagaaat aacccgggat cggtgaccgt
    74701 aagcatcccc gtataatcca cgctactgaa ctggatcagt tgccgggagt tttcccgccg
    74761 cagttgctgt tgtcggtagg catcaaccga gatatccagc agtgtaaaac cacttctctc
    74821 cccctgcgct gccagccagt ccagcgccgc ctgttgttga tgcaaccaga catcacttcc
    74881 ttccgcctgc cccctcacct gccgtttcgc ctccatcagc agatcgtggc gcttgcccgc
    74941 tttacagatt gttggattcg cccgcaggtt gaaacaaagt tgttgtccgg tacacagctc
    75001 gggcgcaaat gaccggcatt ctatggtgaa cgtatcactt tccgccgggc gttcctgcga
    75061 cagtacaaaa aagcgaaacg cgccctggag ttcttcccga cgataaagaa attgcctttc
    75121 tttgccgcta gggaagagat cccacagcca ctgatgcatc acatattcac cgcgatccac
    75181 cagatgcagc aactgcgcag gcgaaagctg gccggtatgt aaggttattc ttgagaggta
    75241 catggttcct ccttgctgag ccacggcccc tgattgacgg tgcgttcccc aaacagccac
    75301 tgctgacgat ttaaaggaac atcccggcgg cgtaatattt tgctcgcgac caggccgtcg
    75361 tgtttccctt cccaccagca ttcatcctga agtttcggga gtgaaacttt cagtttatgg
    75421 aaatgatcct gatactgttg gtatgcatta cataaggcat cacacgcgtt gccttcgagc
    75481 agtaacggcg caagaggtaa tgccagcgga tgactttttc gccccagata aagcggaaaa
    75541 actggatggc gtaaaccgtc ctgcaactgt tcgaggctgt aaggcgcatc ggctgttgtt
    75601 gccaccgcca ccatccacca ggcatcggtg tagtagtcgc gccgggagat aatcgcgctc
    75661 aaaagctcag ggtcgctcaa ctcttcgcga cggctgaaat aacgtgcttt acgcacctct
    75721 tttggcatct ggaccgtgtg ataatcccgt gcccagcgcg ggttacggct ggcgcaaacc
    75781 accagtgaat agtggcgatt aaacgcgttt aagcgttcgg tatcatcacg ccggatccct
    75841 accccggcag ccagcagccc cagcaatgct gagagcgaag gcagttcatg ggtatgacgc
    75901 acttcgccgg gggcatcgac gccccaggat gccatcggcc catgaagctg aaaaatcaga
    75961 tattggctca taagccgccc cttacgcgca gataaagtcc agcacgtcct tcatgcttcc
    76021 ctgtttgttc atcacgtcaa aacttgcgta atcggtcttc tgttcgtaga ccgtattcat
    76081 attttcgcga agagttgtaa tacgctgcac cgccacctct aactgacggg tgccattaat
    76141 gggttcatag aaagctgccg ccagagaacg cggttgatcg gtgccttttt ctgccagcgc
    76201 ccaggaggca taagcccggc tggcaaagct gttttgttta ccggttgggg agactttaag
    76261 cgcggcttcc gtaaaggcgc gtatggtctg attagctaac gcttcgtcac cgccgaggtt
    76321 ttctaccagc agatctttat caatgcagat aagatatagg tgtagaacag cgcagaaccg
    76381 aatccggttt catctacatg accggcaccg gcatcttcag aagcctggcg taaatcatct
    76441 acggcggtga aaaaatcatt ttcgacaatc gtttcactga caccaaacgc atgcgcgacc
    76501 tggcaggcgg cttctacatt aaacccgggt tttttcgcca gcatacgacc aaacatagcg
    76561 atatctaccg ccatacggtc tttacgtaac aaagcgagat ctttctcttt tggcgcgcgc
    76621 ttttcttctg ccagttgatg ggccagcgct tttacggcgt caaattctgc cgggctgata
    76681 tggacgagct gttcagtttc ggcgctagtg agcggatctt ttggtttttt gtcgtttttt
    76741 tgttttttgt cttttttggc tttcccaaga taatcggcta tctctactgc ccattcgatg
    76801 gcttttttat cttcgattcc tttctcaatc aggatagttg ccgcctcacg cgcaatacgc
    76861 ccgctacgaa caccaatatg gcccgccagc gcctgttcaa aaagttcaga agtgcgccaa
    76921 gcacgcttca gactttgcga ggaaacgcgc agacgcgttg ctccacccag aatcacagtt
    76981 ttcggagagc cggaatcatc acggttaaga ttggcagcag ggtaagcggt taacaaatga
    77041 agctgaataa atgttgtcat gagatagtcc tttattaatt ttgttcgtta tcggcgtcgc
    77101 cagcctggta atattccagc gcccagcgcc cagcgcccag cggatacgga taaattctgt
    77161 tggtcgctgc tggcggcggt gatgattgag caggtcatcg ctctcctggc accagcggaa
    77221 gaccccctct tccagagagt caagattgac ggaaccgttt aataatttga ccgcacggcg
    77281 taactgccgc aataaatcat ccggcgtttt tactgccaac aaacgagtaa accgcccctt
    77341 tttcattacc gccgccagtt gcgcggcaaa aggctgccgt tcgtcaatag ctttaacatt
    77401 ggcgctaact gctgctatca gcgctagtgc ggtgatacgc cactcgggtt catcctgcca
    77461 ctttatttgt ctgttcttaa gaaacaggcg aaatccatcc gtcagacaaa catcattaac
    77521 cgtcgtacta cgccgcagac tggcacgctc gccgcgttta tcctgcaact cttcatgcca
    77581 tttacgcagc gcaactttat gctcatcttt tacaatactc attcagcagc ctcctgcttt
    77641 ttctccctgg cggcgttagc attttgcttc tccgctgatg ttgtaaaata tttcttgcgt
    77701 gcggtcatga cgcgttccaa atcaacgggc tcatatggat tggtaaagac acgatcgtca
    77761 aaatcctgac gcgcgaataa ccacatttcc ttttgccatt tactgagtaa ttcgtccgga
    77821 tcctgacctt cttcaatttt gcgcactaat ctcaggaatc gatgctgagt tttgttccag
    77881 aagtcaatat ccacaaaacc gaaatcaagc tttgtggttt tcttctcaga gaaccatgct
    77941 tctttcaacg ctcggtgtaa caagcttaga atgtgtgatg ccgcttgcgc tgccagacgc
    78001 aacttaggta ttaggtcttc ttttttgttt aacaacagcg ggaaatggtg ttcataccaa
    78061 caacgaacct tcatatcttc aaaatcaaaa ccaaatcccc ataagcccac ccttgtctgc
    78121 tttagattgc tggcattcaa aagtttcacg acctgtgcgg ggagttcagt attgtttttt
    78181 gagttaccta cctctatgag gcctaaccag tcgcgccaga ttaaaccacc aggttgtggt
    78241 ttaactgagt aaaaatctcc accctcttta agcggcagac ggtaaggtgt tagaggatgg
    78301 cgccacatga cgtattgaac accatagttt ttgagtgaca tcaagcccaa aagagcatcg
    78361 ctctgcctac cgcaaatgtc gcagttacct attgaggttg tcttaaaatc aatacgtata
    78421 cgtcgtggca ttccccagta ggcctgaagt ttattgactt gttcatccgt taccacagca
    78481 ccgtccagtt cgctggtacg cgtcggtgca agccaaggaa agaccaggtc atcaaaggtt
    78541 tttggtaaag gtaaatcggc ttcatcttgc ggcattacgt ttagccacag ctttcgccac
    78601 aaaggggttt gttgattgcc ctgatactcc agcaattcta taagtgtcgt cagaggccct
    78661 ccgccacgta gcccaggata gtaaccgcgt ccacctatag gtgcattcaa ctggattgaa
    78721 aaaagcgcta aaggcacaca atgcaggcac agatgctgag ttgtgtctcg cttaataaaa
    78781 tggtctgtat ttcgttcttt tgtttgtttt cctggtgcgt caggaagaag cgaagcaata
    78841 aaagttgcct taacttttaa ttcatcaaaa tcctgcataa atgaagggga atctggccca
    78901 aactgaaaag catgttctaa agacagcaat gcttccctta gcttttcagc ttccagtccg
    78961 tcttcccaga tatcatccca actacgttga tcttttggcg cgaagctggt ttgtagtagc
    79021 cccagcaaaa actgccaggc cgccccctgg agatctgccc gcggcgcagc gatatcgaca
    79081 acattttcat cagccagatc gactggtgcc agtttgcctg tcgttccgtc tttaaaacga
    79141 acgggcaacc acggggttgt cagaagtgaa aacgagttca tacgcatcct ctcgtaaaaa
    79201 agaccatccc aggtcggttg tccatccttc catccttttc tgtatatcaa ctattcagag
    79261 agtcttgtcg cagacatctc aaccaacctt gccaatcaat ccctccctgg ccgaataccc
    79321 gcaactttcg tcatcggtga ctaaaatcac gtttgccatt tccggatctt gccgctgttc
    79381 aacgcaccac tgcctgaacg catccccttc cagtaaagaa aactcatccc gatgtttttt
    79441 ccaccagctt cggcgcactc tgacaacgct catttcccat gcgtgagcac cggtggcata
    79501 aggcttcacc acaccgtcaa tacaggtagc cagccacagg gaaacagact cttcagccag
    79561 acgtgtcgac agtttttccg gaaggtaatc gttgatattg gcggcatagc cgagcctgaa
    79621 gttcagcaca tactttttag cccttgcgcg atcacagtaa tatttgccca cttgctcctg
    79681 ctcgctgcgg gcaaatcctt ccggcattac cacgtcctca ccgtaaactg attcaataag
    79741 aagccgggct gcgtgcggca tctgaatagc gccttgctca cgcagcacac gctgcgtcag
    79801 ccagattcgc ccatgatcgg gatagacata tgcactgtta cgcatggcac tgccgaacca
    79861 ttcgtcacca ggagcgtcat cccagacggg ggccagaatc agcaattcag ggggggaacg
    79921 ctcgtctttt ccgtcacgct ttaactgacc attaatatcg cggatatgcc gctgtaatcg
    79981 ccccgctcgc tggattagca aatcaatggg ggccaggtcg gagatcattt cgtccaggtc
    80041 aatatccacg ctttgttcaa ttacttgtgt acaaacaatg acttgagacg ctctctgtaa
    80101 actacaatat ttaccaaagc gtgccagcgt ttccgtttca attcgctggc gatcgctaaa
    80161 agcaaagcgg ctatgaaaaa gtgaaaggct ggaagcagga atgacgccgc gggcaagcag
    80221 ctgacgatac acctgaatag cgtcatcaac agaattccga atccaggcga tgcatttccc
    80281 ctgacttacc gccgattcga tacgcgcaat acactcttgt tcactatgga gccaacccac
    80341 gctcacgcta cgttcaactt ctttgcgcgt cgctacccgg tgcgaatgta catcggtttt
    80401 cgtgacatgc gtcagccagg ggtaatcatc ttttcccagg agtggggctt cttgctggcc
    80461 ctctgcacca cgcgcaaagg cggcgacgag tttgtcgcgc tgctgttgag ataacgtagc
    80521 agaaagcaaa ataacgctgt ttccgccacg cgcctgccgc tcgatcagcc cttcaagaat
    80581 gcacgacata taagcgtcac aggcgtggat ctcatcagcc agcaggattt tgttactcag
    80641 ccccagaagc cgcagattat tatgtttaaa cggcatcact gccatcatcg cctgatccag
    80701 ggtgccgacg ccaatttcag ccagcaacgc cttcttgtta ttgtcggcaa actaggccgc
    80761 acaaccctgg ctgaaagctt gttcatccgg ttcttctgac ccgattaaat caccggacca
    80821 gagtgattca tta
//
LOCUS       DAJTMC010000003        66354 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.003, whole genome shotgun sequence.
ACCESSION   DAJTMC010000003 DAJTMC010000000
VERSION     DAJTMC010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 66354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66354
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.003"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(128..607)
                     /gene="folK"
                     /locus_tag="OO130_000179"
     CDS             complement(128..607)
                     /gene="folK"
                     /locus_tag="OO130_000179"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HCR5363725.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESHILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSLAPEELLNHTQRIELQQDRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDELSKW"
     gene            complement(604..2022)
                     /gene="pcnB"
                     /locus_tag="OO130_000180"
     CDS             complement(604..2022)
                     /gene="pcnB"
                     /locus_tag="OO130_000180"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HCR5363726.1"
                     /translation="MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLL
                     ETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQG
                     KRAWKLLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDE
                     EPSPRRRTRRPRKRAPRREGTA"
     gene            complement(2061..2987)
                     /gene="gluQRS"
                     /locus_tag="OO130_000181"
     CDS             complement(2061..2987)
                     /gene="gluQRS"
                     /locus_tag="OO130_000181"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HCR5363727.1"
                     /translation="MLPPYFLFKEMTDTQYIGRFAPSPSGELHFGSLIAALGSYLQAR
                     ARQGRWLVRIEDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWL
                     HEQGLSYYCTCTRARIQSIVGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDQLRGII
                     HADEKLAREDFIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQISLYQLF
                     GWKVPDYIHLPLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQVEAHWQDFS
                     VEQILQSAVKNWTLTAVPESAIVNSTFSNASC"
     gene            complement(3024..3479)
                     /gene="dksA"
                     /locus_tag="OO130_000182"
     CDS             complement(3024..3479)
                     /gene="dksA"
                     /locus_tag="OO130_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HCR5363728.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3657..4361)
                     /gene="sfsA"
                     /locus_tag="OO130_000183"
     CDS             complement(3657..4361)
                     /gene="sfsA"
                     /locus_tag="OO130_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HCR5363729.1"
                     /translation="MEFSPLLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATRGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMNVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4376..4906)
                     /gene="thpR"
                     /locus_tag="OO130_000184"
     CDS             complement(4376..4906)
                     /gene="thpR"
                     /locus_tag="OO130_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HCR5363730.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALMQ"
     gene            4980..7409
                     /gene="hrpB"
                     /locus_tag="OO130_000185"
     CDS             4980..7409
                     /gene="hrpB"
                     /locus_tag="OO130_000185"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HCR5363731.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLEELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDDAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVSAKNDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIARRRGQDG
                     RYQLANGMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGLLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLELLSPAQSPLQITRDLSAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7611..10133
                     /gene="mrcB"
                     /locus_tag="OO130_000186"
     CDS             7611..10133
                     /gene="mrcB"
                     /locus_tag="OO130_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HCR5363732.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRAHLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGLVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTDTWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWAGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10353..12542
                     /gene="fhuA"
                     /locus_tag="OO130_000187"
     CDS             10353..12542
                     /gene="fhuA"
                     /locus_tag="OO130_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HCR5363733.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAAKHSATATKTDTPIEKTPQSISVVTNEEMQMHQFQSVKEA
                     LGYTPGVTVSSRGASNTYDFVIIRGFSSVGLNQNNYLDGLKLQGNFYNDAVIDPYMLE
                     RVELMRGPTSVLYGKSNPGGIISMVSKRPTTEPLKEIQFKMGTDNLFQTGFDFSDALD
                     DNGEFSYRLTGLARSTNEQQKNSESQRYTIAPSFSWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGASNNTYSRNQKMVGYSFEHGFNDTFTVRQNLR
                     FSEMKTSQKSVYGTGIAGDGHTLNRGTVVDNERLQNFSVDTQLESKFATGDVEHTLLT
                     GVDFMRMRNDINASFGSAPSIDLYNKYHPEYFAFGSAEPYQMNESKQTGIYVQDQAEW
                     NKWVFTLGGRYDWSKQATTVRENSYTPTEGYIERNDHQFTWRGGVNYLFDNGISPYFS
                     YSQSFEPSAFDLWSNPRISYKPSKGEQYEAGVKYVPNDMPVVVTGAVYQLTKTNNLTA
                     DPTNPLAQVPAGEIRARGVELEAKAALNANINLTASYTYTDAEYTKDTNLKGKTPEQV
                     PEHMASLWGDYTFNEGPLSGLTLGTGGRFIGSSYGDPANTFKVGSAAVMDAVVKYDLA
                     RFGMAGSSLAVNVNNLLDREYVASCFQTYGCFWGAERQVVATATFRF"
     gene            12593..13390
                     /gene="fhuC"
                     /locus_tag="OO130_000188"
     CDS             12593..13390
                     /gene="fhuC"
                     /locus_tag="OO130_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HCR5363734.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSSKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRGGEMIAQGTPAEIMRGDTLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13390..14280
                     /gene="fhuD"
                     /locus_tag="OO130_000189"
     CDS             13390..14280
                     /gene="fhuD"
                     /locus_tag="OO130_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HCR5363735.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSAEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14277..16259
                     /gene="fhuB"
                     /locus_tag="OO130_000190"
     CDS             14277..16259
                     /gene="fhuB"
                     /locus_tag="OO130_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HCR5363736.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVAAERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWMWASGALLDDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSIGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLYRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATSTMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16294..17574)
                     /gene="hemL"
                     /locus_tag="OO130_000191"
     CDS             complement(16294..17574)
                     /gene="hemL"
                     /locus_tag="OO130_000191"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HCR5363737.1"
                     /translation="MSKSENLYSAARELLPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPQPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTTRLAEG
                     LLEAAEEAGTPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17799..19220
                     /gene="clcA"
                     /locus_tag="OO130_000192"
     CDS             17799..19220
                     /gene="clcA"
                     /locus_tag="OO130_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HCR5363738.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVVVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSEILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            19244..19309
                     /locus_tag="OO130_000193"
     CDS             19244..19309
                     /locus_tag="OO130_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5363739.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            19302..19646
                     /gene="erpA"
                     /locus_tag="OO130_000194"
     CDS             19302..19646
                     /gene="erpA"
                     /locus_tag="OO130_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HCR5363740.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19693..20316)
                     /locus_tag="OO130_000195"
     CDS             complement(19693..20316)
                     /locus_tag="OO130_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HCR5363741.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20354..21153)
                     /gene="btuF"
                     /locus_tag="OO130_000196"
                     /pseudo
     CDS             complement(20354..21153)
                     /gene="btuF"
                     /locus_tag="OO130_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
     gene            complement(21146..21844)
                     /gene="mtnN"
                     /locus_tag="OO130_000197"
     CDS             complement(21146..21844)
                     /gene="mtnN"
                     /locus_tag="OO130_000197"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HCR5363742.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFEADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21928..23445
                     /gene="dgt"
                     /locus_tag="OO130_000198"
     CDS             21928..23445
                     /gene="dgt"
                     /locus_tag="OO130_000198"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HCR5363743.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRMKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23575..24999
                     /gene="degP"
                     /locus_tag="OO130_000199"
     CDS             23575..24999
                     /gene="degP"
                     /locus_tag="OO130_000199"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HCR5363744.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            25154..26311
                     /gene="cdaR"
                     /locus_tag="OO130_000200"
     CDS             25154..26311
                     /gene="cdaR"
                     /locus_tag="OO130_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HCR5363745.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDAEDHRKRVEQLITRMKEYGRLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIYRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEER"
     gene            complement(26400..26786)
                     /locus_tag="OO130_000201"
     CDS             complement(26400..26786)
                     /locus_tag="OO130_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HCR5363746.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(26948..27760)
                     /gene="yaeI"
                     /locus_tag="OO130_000202"
     CDS             complement(26948..27760)
                     /gene="yaeI"
                     /locus_tag="OO130_000202"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase YaeI"
                     /protein_id="HCR5363747.1"
                     /translation="MISRRRLLQATAATIATSSGFGYMHYWEPGWFELIRHRLAFFKD
                     NAAPFKILFLADLHYSRFVPLSLISDAIALGIEQKPDLILLGGDYVLFDMPLNFSAFS
                     DVLSPLAECAPTFACFGNHDRPVGTEKNHLIGETLKSVGITVLFNQATVIATPNRQFE
                     LVGTGDLWAGQCKPPPASEANLPRLMLAHNPDSKEVMRDEPWDLMLCGHTHGGQLRVP
                     LVGEPFAPVEDKRYVAGLNAFGERHIYTTRGVGSLYGLRLNCRPEVTMLELV"
     gene            complement(27815..28639)
                     /gene="dapD"
                     /locus_tag="OO130_000203"
     CDS             complement(27815..28639)
                     /gene="dapD"
                     /locus_tag="OO130_000203"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HCR5363748.1"
                     /translation="MQQLQNIIETAFERRAEITPTNADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGHVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(28670..31342)
                     /gene="glnD"
                     /locus_tag="OO130_000204"
     CDS             complement(28670..31342)
                     /gene="glnD"
                     /locus_tag="OO130_000204"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HCR5363749.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCVWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(31404..32198)
                     /gene="map"
                     /locus_tag="OO130_000205"
     CDS             complement(31404..32198)
                     /gene="map"
                     /locus_tag="OO130_000205"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HCR5363750.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            32566..33291
                     /gene="rpsB"
                     /locus_tag="OO130_000206"
     CDS             32566..33291
                     /gene="rpsB"
                     /locus_tag="OO130_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HCR5363751.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFEKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            33549..34400
                     /gene="tsf"
                     /locus_tag="OO130_000207"
     CDS             33549..34400
                     /gene="tsf"
                     /locus_tag="OO130_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HCR5363752.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            34547..35272
                     /gene="pyrH"
                     /locus_tag="OO130_000208"
     CDS             34547..35272
                     /gene="pyrH"
                     /locus_tag="OO130_000208"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HCR5363753.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            35564..36121
                     /gene="frr"
                     /locus_tag="OO130_000209"
     CDS             35564..36121
                     /gene="frr"
                     /locus_tag="OO130_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HCR5363754.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            36213..37409
                     /gene="ispC"
                     /locus_tag="OO130_000210"
     CDS             36213..37409
                     /gene="ispC"
                     /locus_tag="OO130_000210"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HCR5363755.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            37597..38355
                     /gene="ispU"
                     /locus_tag="OO130_000211"
                     /gene_synonym="uppS"
     CDS             37597..38355
                     /gene="ispU"
                     /locus_tag="OO130_000211"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HCR5363756.1"
                     /translation="MLSATQLLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQECIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFIDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            38368..39225
                     /gene="cdsA"
                     /locus_tag="OO130_000212"
     CDS             38368..39225
                     /gene="cdsA"
                     /locus_tag="OO130_000212"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCR5363757.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVSLLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            39237..40589
                     /gene="rseP"
                     /locus_tag="OO130_000213"
     CDS             39237..40589
                     /gene="rseP"
                     /locus_tag="OO130_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HCR5363758.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GLQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGAHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            40619..43051
                     /gene="bamA"
                     /locus_tag="OO130_000214"
     CDS             40619..43051
                     /gene="bamA"
                     /locus_tag="OO130_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HCR5363759.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            43173..43658
                     /gene="skp"
                     /locus_tag="OO130_000215"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             43173..43658
                     /gene="skp"
                     /locus_tag="OO130_000215"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HCR5363760.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            43662..44687
                     /gene="lpxD"
                     /locus_tag="OO130_000216"
     CDS             43662..44687
                     /gene="lpxD"
                     /locus_tag="OO130_000216"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HCR5363761.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            44792..45247
                     /gene="fabZ"
                     /locus_tag="OO130_000217"
     CDS             44792..45247
                     /gene="fabZ"
                     /locus_tag="OO130_000217"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HCR5363762.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            45251..46039
                     /gene="lpxA"
                     /locus_tag="OO130_000218"
     CDS             45251..46039
                     /gene="lpxA"
                     /locus_tag="OO130_000218"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HCR5363763.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVGIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFSRSTRGLIR"
     gene            46039..47187
                     /gene="lpxB"
                     /locus_tag="OO130_000219"
     CDS             46039..47187
                     /gene="lpxB"
                     /locus_tag="OO130_000219"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HCR5363764.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNAARDVLGIPHDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVARDFSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKISHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            47184..47780
                     /gene="rnhB"
                     /locus_tag="OO130_000220"
     CDS             47184..47780
                     /gene="rnhB"
                     /locus_tag="OO130_000220"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HCR5363765.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALCEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEHGATEHHRRSFGPVKRALGLAS"
     gene            47817..51299
                     /gene="dnaE"
                     /locus_tag="OO130_000221"
     CDS             47817..51299
                     /gene="dnaE"
                     /locus_tag="OO130_000221"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HCR5363766.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERVKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGVNAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYASGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            51312..52271
                     /gene="accA"
                     /locus_tag="OO130_000222"
     CDS             51312..52271
                     /gene="accA"
                     /locus_tag="OO130_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HCR5363767.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            52370..54479
                     /gene="ldcC"
                     /locus_tag="OO130_000223"
                     /pseudo
     CDS             52370..54479
                     /gene="ldcC"
                     /locus_tag="OO130_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
     gene            54536..54925
                     /locus_tag="OO130_000224"
     CDS             54536..54925
                     /locus_tag="OO130_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR5363768.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            54990..56288
                     /gene="tilS"
                     /locus_tag="OO130_000225"
     CDS             54990..56288
                     /gene="tilS"
                     /locus_tag="OO130_000225"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HCR5363769.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELAQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVPWQTWLQPLELPA
                     GLGSVQLTAGGDIRPPRADEAVSVRFKSAGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(56337..56597)
                     /gene="rof"
                     /locus_tag="OO130_000226"
     CDS             complement(56337..56597)
                     /gene="rof"
                     /locus_tag="OO130_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HCR5363770.1"
                     /translation="MLMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(56584..56784)
                     /locus_tag="OO130_000227"
     CDS             complement(56584..56784)
                     /locus_tag="OO130_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HCR5363771.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            56950..57495
                     /locus_tag="OO130_000228"
     CDS             56950..57495
                     /locus_tag="OO130_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HCR5363772.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            57492..57914
                     /gene="arfB"
                     /locus_tag="OO130_000229"
     CDS             57492..57914
                     /gene="arfB"
                     /locus_tag="OO130_000229"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCR5363773.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTTEQ
                     KARRPTRPTRASKERRLASKAQKSSAKAMRGKVRSGRE"
     gene            57928..58638
                     /gene="nlpE"
                     /locus_tag="OO130_000230"
                     /gene_synonym="cutF"
     CDS             57928..58638
                     /gene="nlpE"
                     /locus_tag="OO130_000230"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308221.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HCR5363774.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERGYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(58838..59658)
                     /locus_tag="OO130_000231"
                     /pseudo
     CDS             complement(58838..59658)
                     /locus_tag="OO130_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414735.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
     gene            complement(59711..61429)
                     /gene="proS"
                     /locus_tag="OO130_000232"
     CDS             complement(59711..61429)
                     /gene="proS"
                     /locus_tag="OO130_000232"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HCR5363775.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(61540..62247)
                     /gene="tsaA"
                     /locus_tag="OO130_000233"
     CDS             complement(61540..62247)
                     /gene="tsaA"
                     /locus_tag="OO130_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HCR5363776.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYHKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(62244..62648)
                     /gene="rcsF"
                     /locus_tag="OO130_000234"
     CDS             complement(62244..62648)
                     /gene="rcsF"
                     /locus_tag="OO130_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HCR5363777.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(62766..63581)
                     /gene="metQ"
                     /locus_tag="OO130_000235"
     CDS             complement(62766..63581)
                     /gene="metQ"
                     /locus_tag="OO130_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HCR5363778.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(63621..64274)
                     /gene="metI"
                     /locus_tag="OO130_000236"
     CDS             complement(63621..64274)
                     /gene="metI"
                     /locus_tag="OO130_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HCR5363779.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(64267..65298)
                     /gene="metN"
                     /locus_tag="OO130_000237"
     CDS             complement(64267..65298)
                     /gene="metN"
                     /locus_tag="OO130_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HCR5363780.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            65486..66058
                     /gene="gmhB"
                     /locus_tag="OO130_000238"
     CDS             65486..66058
                     /gene="gmhB"
                     /locus_tag="OO130_000238"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HCR5363781.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFVLVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAAAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPA"
BASE COUNT        15763 a        16439 c        18206 g        15946 t
ORIGIN      
        1 tgggattgat atttatctgc gggaatttat attatgtttc tgcaaaaatg tttaaactaa
       61 aatttttatt aaggaaagag aggatgtatg attttcagaa aatatttata ttcgtaatat
      121 aaataaatta ccatttgctt aattcgtcaa atgctcttgt atgtaagact tcacgcaacg
      181 tctccccatc aggaaacgcc agctccggtg caatctcaaa cagcggccac agcataaatc
      241 cacgattctt catatcgtag tgcggaacgg tcaggcgttc ggtatttatc acttcattac
      301 caaacagcat gatatcgaga tccagcgtgc gtggtcccca gcgttcagct ttacgaacac
      361 gatcctgttg cagttctatg cgctgtgtat ggttgagtaa ctcttccggg gcgagcgaag
      421 tttcaagcgc cacggcggcg ttgaggtaat cgggttgatc ttgcggcccc agcggtggag
      481 tgcggtaaaa cgaagaaacg gcaagaatgt ggctttcagg gatatcgcct aatgctttca
      541 gggcagcatt gacctgctcc agcggagagg ccagattgct gcctatggca atatacgcca
      601 ctgtcatgcg gtaccctcac gacgcggtgc gcgtttgcgt ggacgacgag tacggcgacg
      661 cggtgacggc tcttcatcca actcgttaag catccctttt tgatctggtg gcgcggaaac
      721 ctggaactca ccccaccatt tcaccagacg ctgcagttca gcgttacgtt caacttcagc
      781 tcgcaaggcc aacaggtcat aagccgcacg gaacttagga tgctccagca gtttccatgc
      841 gcgtttaccc tgacgacggg acatacgcaa ctgcaactgc cagatatcgc gggttaacgt
      901 cgtcagacgt ttcgggattg ccagtgaacg gcaggcttcg tccagcacgt cgttcatcgc
      961 cagcgcgaaa gcgtcgtgat aggtcaggcc gctttcctgg gcgatcttct gtgccgtctc
     1021 cagcagtggg taccagaaca tggcggcaaa caagaacgcc gggttcacgc gcatatcgtt
     1081 atggatacgc gtatcggtat tcttcagcac ctgttcaatg atccgctcca tcgggctgtc
     1141 gccattttcc gtgaagtagc gggtaatggt cgggaacagc ggctggaaca gatgatattc
     1201 acacaacagc ttataggttt cgtaaccgta gcccgcttgt agcagtttaa gcgattcttc
     1261 aaacaggcgt gccggtggga tatcgttcag cagggtagcg aggcgaggga tcggttctgc
     1321 ggtttccggg ctgatgcgca tacccaattt ggcggcaaaa cgtaccgcgc gcagcatacg
     1381 taccggatct tcacggtagc gcgtttccgg gttaccaatc agacggataa cgccgtcctt
     1441 cagatccttc atgccgccaa cgtaatcacg gacggtaaaa tccgctacgc tgtaatacag
     1501 gctgttgata gtgaaatcgc ggcgctgggc gtcttcttcg atggagccga aaatgttgtc
     1561 gcgcagcaac atgccgtttt gcccgcgttg ggaggtcgtg cggtcgctga cgttaccttc
     1621 gtggtgtcca cggaaggttg caacttcgat aatctccggg ccaaacatca catgagccag
     1681 acggaaacgg cgacccacaa ggcggcagtt acggaacagt ttgcgcacct gctcaggcgt
     1741 ggcgttagtg gttacgtcaa aatctttcgg ctttttgcca agtaacaggt cgcgcacgcc
     1801 gccgccaacc agccaggctt cgtatcccgc tttattgagc ctgtacatta ccttcagggc
     1861 attttcactg atatctttgc gggaaatagc atgctgctca cgcgggatca ccgtcacctg
     1921 tggacgggcg actgcctgtt cagcctcgct ttcctcgcgg cttagcacct tgcggcaaaa
     1981 attagcgact cgggtaaaaa tagtacacct cggtagtgtc aaacatcatt caggacaaaa
     2041 aaatagcggc taatcatagc tcagcatgac gcatttgaga atgttgaatt tacaattgcc
     2101 gactcgggca cggcggtcag cgtccagttt ttgacggctg actgaaggat ttgctcgacg
     2161 ctgaaatcct gccagtgtgc ttctacctgc tgccccagaa attgaagtgc cgcgattagt
     2221 accgggcgtg gatcgccttt cggcaacgca ggcgcatgat tctgcttgga aagtttagcg
     2281 ccttgtggat taagcgccag cggcagatga atgtaatctg gcactttcca gccaaaaagc
     2341 tggtacagcg agatttgcct tactgttggt tcaatcagat cagccccacg cactatttct
     2401 gtaacgccct ggaaatgatc atcaaccaca acagccaggt tgtaggcgaa caacccatca
     2461 cggcgatgaa tgataaaatc ttcccgtgcc agtttttcgt cggcgtgaat aatgccgcgc
     2521 agctggtcag taaattgcgt gaccggatgc tgctggcgga tacgcactgc ggcgttgtct
     2581 ggtccatgat gcaacacccg gcaatgaccg tcgtaaatac cgacaatgct ttgaatacgc
     2641 gcacgcgtac aggtgcagta ataacttagt ccttgttcat gtaaccaggc gagtgcttca
     2701 cgataggcgt cgtgacgttg cgattgccag agaacatcgc cgtcccagtg cagaccgtaa
     2761 tgttccagct ggcgcaggat agtttctgcg gcaccgggaa cttcacgagg cgggtcgata
     2821 tcttctatgc gtaccagcca gcgaccttgc cgggcgcgag cctgcaaata gctgccgagc
     2881 gcggcgatca gagagccaaa atgaagctcg ccggaaggag agggggcgaa gcggccaata
     2941 tactgtgtgt ctgtcatctc tttgaacaaa aaataaggcg ggagcatttc ccgcctgtgg
     3001 taaacgtgat ggaacggctg taattagcca gccatctgtt tttcgcgaat ttcagccagc
     3061 gttttgcagt cgatgcacag atcggctgtc gggcgcgctt ccagacggcg aataccaatt
     3121 tcaacaccgc aggattcgca gtagccgaaa tcttcgtctt ccactttttt cagcgtcttc
     3181 tcgatctttt tgatcagctt acgctcgcga tcgcggttac gcagttcgag gctgaactct
     3241 tcttcctggg ctgcacggtc taccgggtcc gggaagttgg ctgcttcatc ctgcatatgt
     3301 gtaacggtgc gatcgacttc atccctgagt tgattacgcc atgcttccag aatacgacgg
     3361 aagtgcgcca gctgggcttc attcatatac tcttcgcccg gcttctcctg atatggttcc
     3421 accccagcga tggcgagaat actcagggac gatgttttac ggttttgccc ttcttgcatg
     3481 ttgcttctcc ttaacacgca ctatcgatcc ccatgttcgg gggaaaaatg aggccgctat
     3541 aaatagcaga tgcttttccg gatagcaatt atctaaacgt aacacttgac aactgtgtga
     3601 ggaaaagcgt atttgcgcac gcgaccagaa tgtaaattaa ccagttactt actttactac
     3661 aatgtaaccg gcagtgattt tttaagagcc atgccttcag cagaaatttc cgctttgtaa
     3721 gccagaattt ctacccccct ctgttgagct tctgacaata gttgcgcgta tttctcatcg
     3781 atgtggcgcg cgggtgaaaa ccgtgtaatg gctgaatgca gcacggcgaa aaagataacc
     3841 gcacgctggc cttcagccgc tacgttcatc aactcccgaa ggtgtttctg acctcgttca
     3901 gtgaccgcat cgggaaaata tccctgttcg ttctccgcta acgtaaccga tttcacttca
     3961 atatagcagt ctggacgcga atccgcctgc aacataaagt caatacggct gcgttctgcg
     4021 ccatatttta cttcgctttt cagcgagcta tagcctgaca gttctgaaat tgattcatta
     4081 aggatagcct ctttcgtcaa cctgttagcc caaagcgtat tgacgcaaat aaatgcgccg
     4141 ctctggcttt gagttagttc ccaggtgtgt gggtatttcc gtttggtgtt gtctgaagtc
     4201 gaataccaga cggtatcgcc acgcgttgca caaccggtca tcgcacccgt attcgggcag
     4261 tgtagcgtta attcgcgacc atcgggtgtg atcacatcgg ctaaaaaacg tttgtaacgc
     4321 tgaattagcg tcgcgcgctg tagaagggga gagaattcca ttgcgacaat ccttgttatt
     4381 gcattagcgc ccagcgtttt agcggcgtgt agcgtgtgcg tccacgggca aacgaggagg
     4441 cgtaaagggt gaactccgtc accgcataag accagttaaa acctggcggc gggattgtca
     4501 ccgcctcgct ggcgtcgcgc aataaggtaa tatgtggatg aaacggacga ttgctttgaa
     4561 aacaaccgct gcgggcagcc tgtgaacgga gcatattcgc cagctggatt aagccgcgtg
     4621 gcggctgacg catccctaac cacaccacac gcgaacgcag ccattgtccg gcgtcatcaa
     4681 gcgtgagtgt gaaaccaggt tgacgaatcc gtccggctaa aagagaaagc gccttctctt
     4741 tctcggcgct cacttcgcct aaaaatgcca gagtcagatg caaattatcg gcggcgaccg
     4801 gacgtcccgc ctcaggtggg aagtgtgtgg cgcgccagtg gataatctgt tcgcggattt
     4861 ctgcaggtaa gtcgatagca aagaacagac gttgcggttc agacatgtga ggcactcggt
     4921 tatgaattac cgcgatgcta caatgtggcg cgaagaatgt taaccctctg gagcgttttg
     4981 tgtcgtcgtt gcccgttgct gccgttttac ctgaattact taccgctctc gattgtgcgc
     5041 cgcaggtatt attaagtgcg ccgaccgggg ccgggaaatc aacctggctg ccgctgcaac
     5101 tgctggcgca tcccggcatt aacgggaaaa ttatcctgct ggagccgcgt cgtctggcgg
     5161 cgcgtaacgt cgcgcaacgg ctggaggagc tgcttaacga aaagccaggc gataccgttg
     5221 gctaccggat gcgtgcgcaa aactgcgtcg ggccgaatac ccgcctggaa gtagttaccg
     5281 aaggcgtgct gacgcggatg atccagcgtg acccggagtt gagcggtgtt ggattggtga
     5341 tccttgatga atttcatgag cgtagcttgc aggcggattt ggcgttggcg ctgttactcg
     5401 atgtgcaaca aggtctgcgt gatgacctta aactgctaat tatgtcggct acgctggaca
     5461 acgaccgctt gcagcaaatg ctgccagaag cgcccgtcgt catctcagaa gggcgctcgt
     5521 ttccggttga acgccgttat ttaccgctgc ccgcgcatca gcgttttgac gatgccgttg
     5581 cggtagccac cgctgaaatg ctgcgtcagg aaagcggatc attactgtta tttttacctg
     5641 gcgtcggaga aattcagcgt gtgcaggaac aactggcttc gcgcatcggc agtgatgtat
     5701 tgctctgccc gctgtatggc gcgttgtcgc tgaacgatca gcgaaaagcg atcctcccgg
     5761 caccgcaagg gatgcgcaaa gtggtgctgg cgaccaatat tgctgaaacc agtttaacca
     5821 tcgaaggtat tcgtctggtg gtggattgtg cccaggagcg tgtggcgcgt tttgatccgc
     5881 gcacggggct aacgcgactg attactcaac gcgttagcca ggcatccatg acgcagcgtg
     5941 ccgggcgcgc cgggcgtctg gagccgggta tctgcctgca tctaatcgcc aaagaacaag
     6001 cagaacgcgc tgccgcgcaa agtgaaccgg aaatcttaca aagcgatctt tccggtttgc
     6061 tgatggaatt actgcaatgg ggatgcagcg atccggcgca gatgagctgg ctggatcaac
     6121 cgccaacggt gaatctactg gccgcgaaac gcctgttaca aatgctgggg gcgctggagg
     6181 gtgaacggct tagtgcgcag gggcaaaaaa tggcagcgct gggtaacgat ccgcgtttag
     6241 cggcaatgct ggttagcgcg aagaacgacg acgaagctgc taccgcggca aaaattgccg
     6301 ccattctcga agagccgcca cggatgggca atagtgacct gggcgtggcg ttttcgcgca
     6361 atcaaccagc ctggcagcaa cgtagtcagc aactgttaaa acgcttaaac gtacgtggcg
     6421 gtgaggcaga cagttcgctt atcgcgccgc tacttgccgg ggcgtttgcc gatcgcattg
     6481 ctcgtcgccg tgggcaagat ggacgctatc aactggcaaa cggcatggga gcgatgctcg
     6541 atgccgacga cgcgctaagc cgccacgaat ggttgatcgc accgttatta ttgcagggca
     6601 gcgcctcgcc ggatgcgcgg attttactgg cgctgccggt cgatattgat gagttagtac
     6661 aacgctgccc gcagctggta cagcagtctg acactgtgga gtgggatgac gcgcaaggta
     6721 cgctgaaagc ctggcgtcgg ctgcaaatcg gtctgctgac ggtgaaagtg cagccgctgg
     6781 cgaaaccctc ggaagacgag ttgcatcagg cgatgcttaa tggcatccgt gataaaggtt
     6841 taagcgtgct caactggacg gcggaagcgg aacagctacg cttgcgtttg ttatgcgccg
     6901 caaagtggtt gccggaatat gactggccag cggttgatga tgaaagttta ttggcgacgc
     6961 tggaaacgtg gctgctgcca catatgactg gcgtacattc actacgcggc ctgaaatcac
     7021 tcgacattta tcaggcacta cgcggattac ttgattgggg aatgcagcaa cgtctggata
     7081 gtgaattgcc tgcgcattac actgtgccga cgggaagccg gatcgccatt cgttatcatg
     7141 aagataaccc gcccgcgctg gcggtgagaa tgcaagagat gtttggcgag gccaccaatc
     7201 cgacgatcgc ccaggggcgc gtgccgctgg tgctggagtt gctttcacct gcccaaagtc
     7261 cattacaaat cacacgagat ttgagcgcct tctggaaagg agcgtaccgt gaggtgcaaa
     7321 aagagatgaa agggcgttat cccaaacatg tctggccgga cgacccggca aatactgcac
     7381 cgacgcgacg gacgaaaaag tattcgtaag gttggttttc tccctctccc tgtgggagag
     7441 ggccggggtg agggcatcag cgcgcacgtt caccctcacc ctaaccctaa ccctctccct
     7501 caagggagag gggaccgatc gaacacaaat tttgagagat atcttcttct gtcttgtaac
     7561 agaagaacag aaaatcgggc ttttgcgcct gaatattgcg gagaaaaagc atggccggga
     7621 atgaccgcga gccaattgga cgcaaaggga aaccgacgcg tccggtcaaa caaaaggtaa
     7681 gccgtcgtcg ttacgaagat gacgatgatt acgacgatta tgatgactat gaggatgaag
     7741 aaccgatgcc gcgcaaaggt aagggcaaag gcaaagggcg taagcctcgt ggcaaacgcg
     7801 gctggctatg gctactgcta aaactggcta tcgtttttgc cgtgctgatc gccatttacg
     7861 gcgtttatct cgatcaaaaa attcgtagcc gtattgatgg caaggtctgg caactgcctg
     7921 cggcagttta tggccgaatg gtcaatcttg agccagatat gaccatcagc aagaacgaga
     7981 tggtgaagct gctggaggcg actcagtatc gtcaggtgtc gaaaatgacc cgtcctggcg
     8041 aatttaccgt gcaggccaac agcattgaga tgatccgccg tccgtttgat ttcccggaca
     8101 gtaaagaagg gcaggtgcgt gcgcatctga cctttgatgg tgatcatctg gcgaccatcg
     8161 tcaatatgga gaacaaccgt cagttcggct ttttccgtct tgatccgcgt ctgatcacca
     8221 tgatctcctc gccaaacggt gagcaacgtc tgtttgtgcc gcgcagtggt ttccctgatt
     8281 tgctggtgga tactttgctg gcgacagaag accgccattt ctacgagcat gatgggatca
     8341 gcctctactc aattgggcgt gcggtgctgg caaacctgac cgcaggtcgc acggtgcagg
     8401 gtgcgagtac gctgacgcaa cagctggtga aaaacctgtt cctctccagc gagcgctctt
     8461 actggcgtaa agcgaacgaa gcctacatgg cgctgatcat ggacgcgcgt tacagcaaag
     8521 accgtattct tgagctgtat atgaacgagg tgtatctcgg tcagagcggc gacaacgaaa
     8581 tccgcggctt cccgctggca agcttgtatt actttggtcg cccggtagaa gagctaagcc
     8641 tcgaccagca ggcgctgtta gtcggtatgg tgaaaggggc gtccatctac aacccgtggc
     8701 gtaacccgaa actggcgctg gagcgacgta atctggtgct gcgtctgctg caacagcaac
     8761 agattattga tcaagaactc tatgacatgt tgagtgcccg tccgctgggg gttcagccgc
     8821 gcggtggggt gatctctcct cagccagcct ttatgcaact ggtgcgtcag gagctgcagg
     8881 caaaactggg cgataaggta aaagatctct ccggcgtgaa gatcttcact acctttgact
     8941 cggtggccca ggacgcggca gaaaaagccg ccgtggaagg cattccggca ctgaagaaac
     9001 agcgtaagtt gagcgatctt gaaactgcga ttgtggtcgt cgaccgcttt agtggtgaag
     9061 ttcgtgcgat ggtgggaggt tctgagccgc agtttgcggg ctacaaccgt gcgatgcagg
     9121 cgcgtcgttc gattggttcc cttgcaaaac cagcgactta tctgacggct ttaagccagc
     9181 cgaaaatcta tcgcctgaac acgtggattg ccgatgcgcc aatcgcgctg cgtcagccga
     9241 atggcctggt ctggtcaccg cagaacgatg accgtcgtta tagcgaaagc ggcagagtga
     9301 tgctggtgga tgcgttgacc cgttcgatga acgtgccgac ggtaaatctg gggatggcgc
     9361 tggggctgcc tgcagttacg gatacctgga tcaaactggg cgtaccgaaa gatcagctgc
     9421 atccggttcc ggcgatgctg ctgggggcgt tgaacttaac gccaatcgaa gtggcgcagg
     9481 cgttccagac catcgccagc ggtggtaacc gtgcaccgct ttctgcactg cgttcggtaa
     9541 tcgcggaaga tggcaaggtg ctgtatcaga gcttcccgca ggcagaacgc gctgttccgg
     9601 cgcaggcggc gtatctgaca ctatggacca tgcagcaggt ggtacaacgt ggtacgggtc
     9661 gtcagcttgg ggcgaaatac ccgaacctgc atctggcagg gaaaacaggg actaccaaca
     9721 ataacgtaga tacttggttt gcaggcattg acggcagcac ggtgaccatc acctgggcag
     9781 gccgtgataa caaccagccg accaaactgt atggagccag cggggcaatg tcgatttatc
     9841 agcgttatct ggctaaccag acgccaacgc cgctgaatct tgttccgcca gaagatattg
     9901 cagatatggg cgtggactac gacggcaact ttgtttgcag cggtggcatg cgtgtcttgc
     9961 cggtctggac cagcgatccg caatcgctgt gccagcagag cgagatgcag cagcagccgt
    10021 caggcaatcc gtttgatcag tcttctcagc cgcaacagca acctgctcag caagagcaga
    10081 aagacagcga cggtgtagcc ggttggatca aggatatgtt tggtagtaat taacatctaa
    10141 gcgtgaaata ccggatggcg agttgccatc cggtaaaata acatcccatc taagatatta
    10201 accctttctt ttcatctggt tgtttattaa cccttcagga acgctcagat tgcgtaccgc
    10261 ttgcgaaccc gccagcgttt cgaatattat cttatcttta taataatcat tctcgtttac
    10321 gttatcattc actttacatc agagatatac caatggcgcg ttccaaaact gctcagccaa
    10381 aacactcact gcgtaaaatc gcagttgtag tagccacagc ggttagcggc atgtctgttt
    10441 atgcacaggc agcggttgaa ccgaaagaag acactatcac cgttaccgct gcacctgcgc
    10501 cgcaagaaag cgcatggggg cctgctgcaa ctattgcggc gaagcactct gctactgcga
    10561 ctaaaacgga tacaccaatt gaaaaaacgc cgcagtctat ttcggttgtg acaaatgaag
    10621 agatgcagat gcatcaattt cagtctgtaa aagaagcatt aggttataca cctggcgtta
    10681 cggttagcag tcgtggtgct tctaatacat atgattttgt tatcattcgt ggtttctcat
    10741 ctgttggtct gaatcaaaat aattaccttg atggactaaa acttcagggc aacttttata
    10801 acgatgctgt gattgatcct tacatgctcg agagggttga actgatgcgt gggccgacgt
    10861 ctgttctcta tggaaaaagt aatcctggtg ggattatttc tatggtgagt aagcgtccga
    10921 ccactgaacc cctgaaagaa attcagttta aaatggggac ggataatctg tttcagaccg
    10981 gattcgattt cagtgatgca ctggatgata acggtgagtt ctcttaccgt ttgacgggcc
    11041 tcgctcgctc aacaaacgaa cagcagaaaa actctgaatc tcagcgttat accattgctc
    11101 catcattctc atggcgtcca gacgacaaaa ccaattttac tttcctgtcc tatttccaga
    11161 atgaaccaga aacgggttat tacggttggt tgccgaaaga ggggaccgtt gagccattgc
    11221 ctaatggtaa gcgtctaccg actgacttca atgaaggtgc atcgaataat acatactccc
    11281 gtaaccagaa aatggtggga tatagttttg aacatggttt taatgacacc ttcaccgtgc
    11341 gtcagaatct gcgtttcagt gaaatgaaaa cctcacagaa aagtgtttat ggcacaggga
    11401 ttgctggtga tggtcatacc ctaaaccgcg ggacagtggt ggataatgag cgtctacaaa
    11461 actttagcgt tgatacccaa cttgaaagta agtttgctac aggtgacgtc gagcatactt
    11521 tgctgacagg ggtagacttc atgcggatgc gtaatgatat caatgccagc tttggatccg
    11581 caccatccat cgatctttat aacaaatatc accctgaata ctttgcattt ggtagcgcag
    11641 agccatacca gatgaatgaa agcaaacaaa caggtattta tgttcaggat caggcggaat
    11701 ggaataaatg ggtattcact ctggggggac gctacgattg gtctaagcaa gcgaccactg
    11761 ttcgtgaaaa ctcttatacg ccgactgaag gttatattga gcgcaatgat catcagttca
    11821 cctggcgcgg tggtgtaaat tacttattcg ataatggtat ttcaccttac tttagctata
    11881 gccagtcctt tgaaccgagt gctttcgatc tgtggagcaa cccacgcatt tcctataagc
    11941 catcgaaagg tgaacagtat gaagctggcg taaaatatgt tccgaatgat atgccggtcg
    12001 ttgttacggg cgcagtctat caattgacga aaacaaataa cctgacagca gacccaacaa
    12061 acccattagc gcaagtccca gcaggtgaga ttcgcgctcg tggtgtggaa cttgaagcaa
    12121 aagcggcgtt aaatgccaat attaacttga cggcttctta tacctacacc gatgcggaat
    12181 acaccaaaga caccaatctc aaaggtaaaa ctccagaaca agtaccggag catatggcat
    12241 ctctctgggg ggattatacc ttcaatgaag ggccgctttc tggtttaaca ttgggaacag
    12301 gtggtcgttt tattggttcc agctatggtg atccggcaaa cacttttaaa gtgggtagcg
    12361 cagctgtaat ggatgctgtt gtaaaatatg atctggcacg ctttggtatg gcgggatcca
    12421 gccttgctgt gaacgtcaac aatttgctcg atcgtgagta tgttgccagt tgcttccaga
    12481 cttatggctg cttctggggc gcagaacgtc aggtcgttgc aaccgcaacc ttccgtttct
    12541 aatttctctt ttggggcacg gatttccgtg cccatttcac aagttggctg ttatgcagga
    12601 atacacgaat cattccgata ccacttttgc actgcgtaat atctcctttc gtgtgcccgg
    12661 gcgcacgctt ttgcatccgc tgtcgttaac ctttcctgcc gggaaagtga ccggtctgat
    12721 tggtcacaac ggttctagta aatccactct gctcaaaatg cttggccgtc atcagcctcc
    12781 gtcagaaggg gagattcttc ttgatgccca gccgctggaa agctggagca gcaaagcgtt
    12841 tgcccgcaaa gtggcttatt tgccgcagca gcttcctccg gcagaaggga tgaccgtgcg
    12901 tgaactggtg gcgattggtc gttacccgtg gcatggcgcg ctggggcgct ttggggcggc
    12961 agatcgcgaa aaagtcgagg aagctatctc gctggttggc ttaaaaccgc tggcgcatcg
    13021 gctggttgat agtctctctg gcggcgaacg tcagcgggcg tggatcgcca tgctggtggc
    13081 gcaggatagc cgttgtctgt tgctcgacga accgacctcg gcgctggata tcgcccacca
    13141 ggttgatgtg ctggcgctgg tgcaccgttt aagtcaggag cgtggcctga cggtcattgc
    13201 cgtgttgcac gatatcaata tggcggcacg ctactgtgat tatctggtcg ccctgcgcgg
    13261 tggtgaaatg attgctcagg gaacgcctgc ggaaattatg cgcggcgata ccctcgaaat
    13321 gatttatggc atcccgatgg gtattttgcc gcatccggcg ggtgctgcac ctgtgagttt
    13381 tgtttattga tgagcggctt acctcttatt tcgcgccgtc gactgttaac ggcgatggcg
    13441 ctttctccgt tgttatggca gatgaatacc gcccacgcgg cggctattga tcccaatcgt
    13501 attgtggcgc tggagtggtt gccggtggaa ttactgctgg cgctcggcat cgtgccttac
    13561 ggcgtagcgg ataccatcaa ctatcgcctg tgggtcagcg aaccaccatt gccggactca
    13621 gtgatcgacg tcggtttgcg cacagaacct aaccttgaac tgctgaccga aatgaaaccg
    13681 tcgtttatgg tctggtcggc aggatatggc ccttcagcag aaatgctggc acgtattgcg
    13741 ccggggcgtg gatttaactt cagcgacggc aaacagccgc tggcgatggc gcgtaaatca
    13801 ctgacggaaa tggcagattt acttaacctg caaagcgcag cagaaacgca tttagcgcaa
    13861 tatgaagact ttatccgcag catgaaaccc cgctttgtga agcgtggtgc acgcccgtta
    13921 ttgctgacga cgcttatcga tccgcgccat atgttggtct tcggtccaaa cagcttgttc
    13981 caggaaattc ttgatgagta cggcatccca aatgcctggc aaggggaaac caacttctgg
    14041 ggcagtaccg ccgtcagtat cgatcgtctg gcagcgtata aagacgttga tgtgctctgt
    14101 tttgatcacg acaacagcaa agacatggat gcgctaatgg caaccccgct gtggcaggcc
    14161 atgccgtttg tccgcgccgg acgctttcag cgcgtacccg cagtctggtt ttatggtgcg
    14221 acgctctcgg caatgcactt tgtgcgcgtt ctggataacg ccatcggagg taaagcgtga
    14281 gtaaacgaat tgcgcttttc ccggcgttat tgctggcgct gctggtgatt gtcgctacgg
    14341 cgctgacctg gatgaacttt tcgcaggcgc tgccgcgtag ccagtgggcg caggccgcct
    14401 ggtcgccgga tattgacgtc atcgagcaga tgatttttca ctacagcttg ctgccgcgtc
    14461 tggcgatttc gctgctggtg ggcgcaggcc tggggctggt gggcgtgctg tttcagcaag
    14521 tgctgcgtaa tccgctggcg gaaccgacga cacttggcgt tgctacaggc gcgcaactgg
    14581 ggattaccgt cactacgctc tgggcgatcc caggggcgat ggcgagtcag tttgctgcgc
    14641 tggcgggcgc ttgcgttgtt ggcttaatcg tctttggcgt cgcgtggggg aaacggcttt
    14701 cgccggtaac gctgatcctc gcggggctgg tagtgagcct ttattgtggg gcaatcaatc
    14761 agttactggt tatcttccac catgaccaac tgcaaagcat gttcctgtgg agcaccggaa
    14821 cgctgacgca aaccgactgg ggcggtgttg agcgtttatg gccgcagttg ctgggcggtg
    14881 tgatgctgac gttgctgcta cttcgcccgt taaccctgat ggggcttgat gatggcgtgg
    14941 cgcgcaatct cgggctggcc ttgtcgctcg cgcgtctggc ggcgctgtcg ctggcgattg
    15001 tcatcagtgc gctgctggtg aacgcggtgg ggattatcgg ctttatcggg ttgtttgcgc
    15061 cgttgctggc aaaaatgctg ggggcgcggc gtctgctgcc gcgactgatg ctggcgtcgt
    15121 tgattggtgc gctgatcctc tggctttccg atcaaatcat cctctggctg actcgcgtgt
    15181 ggatggaagt gtccaccggt tcggtcactg cgttgatcgg tgcgccgctg ctactgtggc
    15241 tgttgccgcg tttacgcagc attagcgcgc cggatatgaa ggtcaacgat cgtgtcgcgg
    15301 ctgaacgcca acatgtgctg gcgtttgccc tcgcgggcgg cgtgctgctg ttgatggctg
    15361 tggtggtggc gctgtcgttt ggtcgtgatg cgcacggctg gatgtgggcg agcggggcgt
    15421 tgctcgatga tttaatgccc tggcgctggc cgcgaattat ggcggcgcta tttgcgggcg
    15481 tcatgctggc ggtggcgggc tgtattattc agcgactgac cggcaacccg atggcaagcc
    15541 cggaagtgct ggggattagc tccggcgcag cgtttggcgt ggtactgatg ctgtttctgg
    15601 taccgggtaa tgcttttggc tggctgttac ccgcgggaag tatcggtgcc gcggtgacgc
    15661 tgttgattat tatgatcgcc gccgggcgcg gtggattttc cccacaccgc atgttactgg
    15721 cggggatggc gttaagtacc gcattcacca tgcttttaat gatgttgcag gcgagtggtg
    15781 acccgcgaat ggcgcaagtg ctcacctgga tctctggttc gacctacaac gccaccgatg
    15841 cgcaggtctg gcgtaccgga attgtgatgg tgattttgct ggcgattacc ccgctgtacc
    15901 gccgctggct gaccatttta cctctgggcg gtgataccgc acgagctgtg ggaatggcgc
    15961 tgacgccgac gcgaattgcg ctgctgctgt tagcggcttg cctgacggcg acctcgacga
    16021 tgactattgg accgttgagt tttgttggtt taatggcacc gcatattgcg cggatgatgg
    16081 gttttcgacg gacgatgcca cacatcgtaa tttcggcgct ggtgggtggt ttactgctgg
    16141 tattcgctga ctggtgtggg cggatggtgc tgtttccatt ccagatcccg gcggggctgc
    16201 tgtcgacctt tatcggcgcg ccatatttta tctatttgtt gagaaagcag agccgttaat
    16261 tgagtgcctc gcccggtggg cgaggcaaaa ttatcacaac ttcgcaaata cccgacgtgc
    16321 agcatcgatg gtgttattga tatcttccat gctgtgcgcc acagacataa agcccgcttc
    16381 aaacgctgac ggtgccaggt aaacaccttc gtccagcatc atatggaaga aacgcttaaa
    16441 gcgttccacg tcacaggcca tcacatcctg atagcacgtc acggactcgg cgtcggtaaa
    16501 gaaaataccg aacatgccgc caacgtggtt aacgaccagc ggagttccgg cttcttctgc
    16561 cgcttccagc agaccttctg ccagacgtgt tgtcagctca tccagcgttt cgtgaacgcc
    16621 cggctgcgcg acttcattca gacaggcgaa acccgctgcc atcgcaatcg ggttaccgga
    16681 aagcgtaccc gcctgataga ccggacccgt cggggccagc gcatccatta catcacgacg
    16741 accaccgaat gcgcctaccg gcattccacc gccgatgatt ttgccgaggc aggttaaatc
    16801 tggcactacg ccgtaataat cctgtgcgcc agccagtgcc acgcggaagc cggtcatcac
    16861 ttcatcgatg atcagcaacg cgccaaattc gtcgcacagc gcacgcagac ctggcaggaa
    16921 ctctggctgc ggcggaacac agttcatatt gcctgccacc ggctcgacga taatacaggc
    16981 aatctcttgc gggtattgct caaacgcggc gcgtacagaa gccagatcgt tataagtaca
    17041 ggttaaggta tgtttggcga aatctgccgg aacacccggc gagtttggct ggcctaacgt
    17101 gagtgcgcca gaaccggctt tcaccagcag gcagtcagcg tgaccgtggt aacaaccttc
    17161 aaatttaatg attttgtcgc gaccggtaaa accacgggcc aggcggatgg cgctcatggt
    17221 cgcttcagtg ccggagttca ccatgcgcac catatccatg gtcgggacca gttcggtcac
    17281 cagttgcgcc attttcactt ccatttcggt cggtgcacca aagcttaaac cacgctcggc
    17341 ggcttcaatc acggcattgc ggatagccgg atggttatgg cccagcacca tcggccccca
    17401 ggaaccgaca taatcgatat aggctttgcc atcaacatcg tacagataag cgccgtccgc
    17461 tttttcgata aacagtggag tgccgcccac gccagtaaag gcgcgaacag gggagttcac
    17521 accgccaggg agcagctcgc gcgctgcgct gtaaagattt tcagacttac tcatggaggg
    17581 ttcctgattc gtagaaaaag tgaatggctg ctattctatg ttattcataa caagttaaat
    17641 actcgtcaaa catcaggctg cttgtactgg tgcaatcctg atttcttaga gtataaaagt
    17701 tttgtgcatt tgaaacatta cgctttgcaa aggattttca tggaacgtgc gagtaaaatg
    17761 ccgtcatctt atttgtatga ccaataagtg atcattggat gaaaactgat actccctctt
    17821 tagaaacacc gcaggccgcg cgcctgcgac gcagacaact gattcgccaa cttcttgagc
    17881 gcgataaaac cccgttagcc attttgttta tggcggcagt cgtcggcacg cttgtcgggc
    17941 tggcagcggt tgcttttgac aaaggtgtcg cctggttgca gaaccaacgt atgggggcgc
    18001 tggtacatac tgctgataat tatccgcttc tgttaaccgt cgcttttctc tgttcggcgg
    18061 tgctggcgat gttcggctac tttttggtgc gcaaatacgc gccggaagca ggtggttcgg
    18121 ggatcccgga aattgaaggg gcgctggaag atcaacgtcc cgttcgctgg tggcgtgtgt
    18181 tgccggtgaa attctttggc gggctgggga cactcggcgg aggcatggtg ctggggcgcg
    18241 aagggccaac cgtgcagatc ggcggcaata tcggtcgcat ggtactcgat attttccgcc
    18301 tgaaaggtga tgaagctcgc catacgctgc tggcaaccgg tgctgctgcg gggctggctg
    18361 cggcctttaa cgcgccgctg gcgggtattt tgtttattat cgaagagatg cgtccgcagt
    18421 ttcgctatac gttaatttcg attaaagcgg tatttattgg cgtcattatg tcgaccatta
    18481 tgtaccggat ttttaatcat gaagttgcgt tgattgacgt cggtaaactt tctgatgcgc
    18541 cgcttaatac gctgtggctt tatctgatcc tcggtattat ttttggcatt ttcggcccta
    18601 tttttaataa atgggtgctg gggatgcagg atttgctgca ccgtgtgcac ggcggcaata
    18661 ttaccaaatg ggtgctaatg ggcggtgcga ttggcggtct gtgtggattg ctggggtttg
    18721 tggcaccagc aacgtcgggc ggcggtttta acctgattcc tatcgctacc gcagggaatt
    18781 tcagcatggg aatgctggtg tttatcttcg tcgcgcgggt cattaccacc ttactgtgct
    18841 tctcttccgg cgcgccgggc ggtatttttg ccccgatgct ggcgctgggt actgtgctgg
    18901 gaaccgcttt cggaatggtt gtcgttgagc tgtttccgca atatcacctt gaggcgggga
    18961 cttttgctat tgccggaatg ggggcgttac tggcggcatc tattcgcgcg ccgttaacgg
    19021 ggatcatttt ggttctggag atgaccgata actaccagct cattttgcca atgattatta
    19081 ccggtcttgg cgcaacacta ttagcgcaat ttaccggcgg gaaaccgcta tattcggaga
    19141 ttcttgcgcg cacgctggca aaacaggaag ctgagcaact ggcgcgaagc aaggccgcat
    19201 cagccagcga gaatacttga acgaaatacc agggtattag ataatggcga ttattattgg
    19261 gttagaattt gcccaattgc cgatgtcgtt tggagcaaaa tatgagtgat gacgtagcac
    19321 tgccgctgga gtttaccgac gcagcagcca acaaagttaa aagcctgatc gctgacgaag
    19381 ataacccgaa tctgaaatta cgcgtgtata tcaccggtgg cggttgcagc ggcttccagt
    19441 atggtttcac ctttgatgat caggtgaacg aaggcgatat gaccatcgaa aaacagggcg
    19501 ttggcctggt ggttgatccg atgagcctgc aatatctggt cggtggttcc gttgactata
    19561 ccgaaggact ggaaggttct cgtttcatcg tgaccaaccc gaacgcgaaa agcacctgcg
    19621 gttgcggttc ttcctttagc atctaatctg ttgttcatgt gccggataaa ctatccggca
    19681 catcttctct cttcaacgcc cattctcatc cagcgcaaac gtcggtagct taagatgcca
    19741 acgaatagcc gccagccgaa tcaatagcgt cacgaccatg cccatcatac tggctgtttc
    19801 cagtggtacg gaaaatgtgt aataagccgt agcgtggaca ataccgccga taatacaggc
    19861 agttgcgtag atttctgtac gtaaaatcat ggggatttcg cgggccagta catcacgaat
    19921 gatcccgccg ccaacgccag taatgacgcc catacaaacc gcgattaacg gaccggcttc
    19981 cgcattaaag gctttattca cgccaatgcc gacaaacacc gccagaccaa cggcgtccag
    20041 caccggcaac atccattttg gtaagcgtct tggctggcgc accagcacga tggtcagcat
    20101 gctggttacc attgcaacga ccagatcggt gggatctttc acccaaaata ccgggccgtg
    20161 atccagcgcc atgtcgcgaa ttgtcccgcc gcctacagcg gtaaccacgc ccagtaccag
    20221 aacaccaaaa gggtccatac gcaattttcc ggctaacaaa acgccggaga tggcaaatac
    20281 cgctgtgccg actatatcca gccaatagac gagcattgtt caatccccac tgagcaccta
    20341 aaaaggtcag gcgctaatct acctgtgaaa gcgcattaca gagctgttgt gcagcgagga
    20401 taatacgtgg gcttgcacgt caaaccagtc actcgtgaga ggaataacgg gaattttgag
    20461 ctgttcaccc cagtattgtt tgattttagg aatttggtcc ggtccgcctg taatgacaat
    20521 cgcctgtggc gagcgtgcta acacctgttc gcggctaact tgcggccaag gaacccggct
    20581 gtctttaaag atgttttctc cgccacaaac ttcgagtacc tggttttgaa tcgactcttt
    20641 tccactggta aatggcggat taatgccgaa ttgcagaaaa acacgttttt taggtttatc
    20701 agcatattgc gctttcaatt gcgcgtactg atccagcagg gattgcgcgg cttgttcggc
    20761 cttatccggt tgcggactcc agggggccag ttgacgtaac gcattggcaa tttgttcaat
    20821 gcttgtcgca tcgacccaca tcacttttat tcccggcgaa gccagctggt caacctgccg
    20881 ctcggcatta cctccacgcc aggcaatcac cagatcgggt ttcagcgcga caatgcgttc
    20941 cagattcatc ccctgccagg tggaaacctg ctcaatcttt tgcgcttgtg gaggatagtc
    21001 ggaatagctg ctgaccccaa ccggcgtgat cccggcggca aaggcaagtt cagtgttggc
    21061 gggagaaagc gtgatgacgc gcggtgccgc gttgagccac agtggcgcga gaaaagacag
    21121 ggcgaccagc gccctgaaca gtgacttagc catgtgccag tttctgcacc agtgactcaa
    21181 ccatcaggct ggactgttta gcggcaacag ccaggaactc atcgaagcta agatgagact
    21241 gttgatcggc cacgtcggag atggcgcgta cgacaacaaa cgggacgttg aaattgtggc
    21301 agacatgggc gattgccgtc gcttccatct ctacagcaat ggcctgtggg aagttgtggc
    21361 ggattttcgc cagaccaaca gaaccgttga tgaaagcgtc gccgctaaca atcaggccac
    21421 gtacagcgtt aagattcagt tcggcaatgc aggcctcagc ggcagcgatc agtttatcgt
    21481 cagcttcaaa gcctgccgga cagcctggta actgaccgta ttcataacca aatgccgtga
    21541 catccgcgtc gtgataacgt gcttcgtccg agacaacgat atcgcccact ttcaacgttg
    21601 gtgccaggcc accggcagaa ccggtgttaa taatcacatc tggcttgcag tgttccaaca
    21661 gcaaagtggc acccagcgcc gcagcgactt taccgatgcc cgatttcaga agcgcaacct
    21721 cggttccatt cagttggccg gtatagattt cgcaaccgcc gagactgata gtttgacgat
    21781 tttcgatttt gtcacgcagc agcgtaactt cttcttccat tgcaccaatg atgccgattt
    21841 tcatagattt actcgcgata agcccgattt gaaggcatag tttaccatgc gcttacgggg
    21901 aagcgtattt ctcacgcggg agaggacatg gcacagattg atttccgaaa aaaaataaac
    21961 tggcatcgtc gttaccgttc accgcagggc gttaaaaccg aacatgagat cctgcggatc
    22021 ttcgagagcg atcgcgggcg tatcatcaac tctccggcaa ttcgtcgtct gcaacaaaag
    22081 acccaggttt ttccactgga gcgcaatgcc gccgtgcgca cgcgtcttac ccactcgatg
    22141 gaagtccagc aggtggggcg ctacatcgcc aaagaaattc taagccgtat gaaagagctt
    22201 aaattactgg aagcatacgg cctggatgaa ctgaccggcc cctttgaaag cattgttgag
    22261 atgtcatgcc tgatgcacga tatcggcaat ccgccgtttg gtcattttgg cgaagcggcg
    22321 ataaatgatt ggtttcgcca gcgtttgcac ccggaagatg ccgaaagcca gcctctgact
    22381 gacgatcgct gcagcgtggc ggcactacgt ttacgggacg gggaagaacc gcttaacgag
    22441 ctgcggcgca agattcgtca ggacttatgt cattttgagg ggaatgcaca aggcattcgt
    22501 ctggtgcata cattgatgag gatgaatctc acctgggcac aggttggcgg tattttaaaa
    22561 tatacccgtc cggcgtggtg gcgtggcgaa acgcctgaga cacatcacta tttaatgaaa
    22621 aagccgggtt attatctttc tgaagaagcc tatattgccc ggttgcgtaa agaacttaat
    22681 ttggcgcttt acagtcgttt tccattaacg tggattatgg aagcagccga cgacatctcc
    22741 tattgtgtgg cagaccttga agatgcggta gagaaaagaa tatttaccgt tgagcagctt
    22801 tatcatcatt tgcacgaagc gtggggccag catgagaaag gttcgctctt ttcgctggtg
    22861 gttgaaaatg cctgggaaaa atcacgctca aatagtttaa gccgcagtac ggaagatcag
    22921 ttttttatgt atttacgggt aaacacccta aataaactgg taccctatgc ggcacaacga
    22981 tttattgata atctgcctgc gattttcgcc ggaacgttta atcatgcatt attggaagat
    23041 gccagcgaat gcagcgatct tcttaagcta tataaaaatg tcgctgtaaa acatgtgttt
    23101 agccatccag atgtcgagca gcttgaattg cagggctatc gggtcattag cggattatta
    23161 gagatttatc gtcctttatt aagcctgtcg ttatccgact ttactgaact ggtagaaaaa
    23221 gaacgggtga aacgtttccc tattgaatcg cgcttattcc acaaactctc gacgcgccat
    23281 cggctggcct atgtcgaggc tgtcagtaaa ttaccgtcag attctcctga gtttccgcta
    23341 tgggaatatt attaccgttg ccgcctgctg caggattata tcagcggtat gaccgacctc
    23401 tatgcgtggg atgaataccg acgtctgatg gccgtagaac aataaccagg cttttgtaaa
    23461 gacgaacaat aaatttttac cttttgcaga aactttagtt cggaacttcg cgctataaaa
    23521 cgaatctgaa gaacacagca attttgcgtt atctgttaat cgagactgaa atacatgaaa
    23581 aaaaccacat tagcactgag tgcactggct ctgagtttag gtttggcgtt atctccgctc
    23641 tctgcaacgg cggctgagac ttcttcagca acgacagccc agcagatgcc aagccttgca
    23701 ccgatgctcg aaaaggtgat gccttcagtg gtcagcatta acgtagaagg tagcacaacc
    23761 gttaatacgc cgcgtatgcc gcgtaatttc cagcagttct tcggtgatga ttctccgttc
    23821 tgccaggaag gttctccgtt ccagagctct ccgttctgcc agggtggcca gggcggtaat
    23881 ggtggcggcc agcaacagaa attcatggcg ctgggttccg gcgtcatcat tgatgccgat
    23941 aaaggctatg tcgtcaccaa caaccacgtt gttgataacg cgacggtgat taaagtccag
    24001 ctgagcgatg gccgtaagtt cgacgcgaag atggtcggca aagatccgcg ctctgatatc
    24061 gcgctgatcc aaatccagaa cccgaaaaac ctgaccgcaa ttaagatggc ggattctgat
    24121 gcactgcgcg tgggtgatta caccgtagcg attggtaacc cgtttggtct gggcgagacg
    24181 gtaacttccg ggattgtctc tgcgctgggg cgtagcggcc tgaatgctga aaactacgaa
    24241 aacttcatcc agaccgatgc agcgatcaac cgtggtaact ccggtggtgc gctggttaac
    24301 ctgaacggcg aactgatcgg tatcaacacc gcgatcctcg caccggacgg cggcaacatc
    24361 ggtatcggtt ttgcaatccc gagcaacatg gtgaaaaacc tgacctcgca gatggtggaa
    24421 tacggccagg tgaaacgcgg tgagctgggt attatgggga ctgagctgaa ctccgaactg
    24481 gcgaaagcga tgaaagttga cgcccagcgc ggtgctttcg taagccaggt tctgcctaat
    24541 tcctccgctg caaaagcggg cattaaagcg ggtgatgtga tcacctcact gaacggtaag
    24601 ccgatcagca gctttgccgc actgcgtgct caggtgggta ctatgccggt gggcagcaaa
    24661 ctgaccctgg gcttactgcg cgacggtaag caggttaacg tgaacctgga actgcagcag
    24721 agcagccaga atcaggttga ttccagctcc atcttcaacg gcattgaagg cgctgagatg
    24781 agcaacaaag gcaaagatca gggcgtggta gtgaacaacg tgaaaacggg cactccggct
    24841 gcgcagatcg gcctgaagaa aggtgatgtg attattggcg cgaaccagca ggcagtgaaa
    24901 aacatcgctg aactgcgtaa agttctcgac agcaaaccgt ctgtgctggc actgaacatt
    24961 cagcgcggcg acagcaccat ctacctgtta atgcagtaat ctcactcaac cccttcctga
    25021 aaacgggaag gggttctcct tacaatctgt gaacttcacc acaactccat acatcttcat
    25081 catcctttag gcatttgcac aatgccgtac gttacgtact tccttatgct aagccgtgca
    25141 taacggagga cttatggctg gctggcatct tgataccaaa atggcgcagg atatcgtggc
    25201 acgtaccatg cgcatcatcg ataccaatat caacgtaatg gatgcccgtg ggcgaattat
    25261 cggcagcggc gatcgtgagc gtattggtga attgcacgaa ggtgcattgc tggtactttc
    25321 acagggacga gtcgtcgata tcgatgacgc ggtggcacgt catctgcacg gtgtgcggca
    25381 agggattaat ctaccgttac ggctggaagg tgaaattgtc ggcgtaattg gcctgacagg
    25441 tgaaccagag aatctgcgta aatatggcga actggtctgc atgacggctg aaatgatgct
    25501 ggaacagtcg cggttgatgc acttgttggc gcaggatagc cgtttgcggg aggaactggt
    25561 gatgaacctg attcaggcag aggagaatac tcccgcactt actgaatggg cgcaacggct
    25621 ggggatcgat ctcaatcaac cgcgagtggt ggctattgtt gaggtcgaca gcggtcagct
    25681 tggtgtggac agcgcaatgg cggagttaca acaactgcaa aacgcgctga ctacgcccga
    25741 gcgtaataat ctggtggcga ttgtctcgct aaccgaaatg gtggtgttga aaccggcgtt
    25801 gaactctttt gggcgctggg atgcagaaga tcatcgtaag cgagttgaac aactgattac
    25861 ccgcatgaaa gagtacggcc ggctgcgttt tcgcgtttca ctgggcaact attttaccgg
    25921 tcctggcagt attgcccgat cctatcgtac ggcgaaaacg acgatggtgg tgggtaaaca
    25981 gcggatgcca gaaagtcgct gctattttta tcaggatctg atgttacctg tgttactcga
    26041 cagtttgcgt ggcgactggc aggccaacga actggcgcga ccgctggcgc ggctgaaagc
    26101 gatggacaat aacggcttgc tgcgacgaac gctggcggcg tggtttcgtc acaatgtgca
    26161 accgctggca acgtcaaagg cgttgtttat ttatcgtaat accctggagt atcggcttaa
    26221 tcgtatatcg gaactgaccg ggcttgattt gggcaatttt gatgacaggt tgctgctgta
    26281 tgtggcgtta caactggatg aagagcggta ggttatgcgt taaggttgag gcggcgctgt
    26341 ctcattcccc tcagcccgaa ggggagaggg gaatgcccga attaaccgtc aattcgggat
    26401 tatttacgcg ttagtttttc cagatcggct tcaatctcgc tgatcttatt ggttacgact
    26461 gactccaggt gacgtaagtc gtcgaggatc ttacgcttaa gatcaacttc gctgcggtca
    26521 cgctggcaga tttgatcaag ctcatcaatg atataccgta gattcgggct gatttcctgg
    26581 acttctttat aaccctgacc cacaccatca gcgacgaccg ttttacgctg acgcggatat
    26641 ttaaacttaa cgctcttggc gaaaaactcg cctttgtctt tctggaaata gattttcaga
    26701 atatcgttgt tggcttcctg ccggaggctg taacgatcaa tttcttcagg attggtaata
    26761 cccagacttt tcagattgtc gtacatagcg ttacctcaaa atgagtcagt aaattgtgct
    26821 tattttagca tttggcctag cccgcccggc tcgactgtga tcgcagaaag cctttttctt
    26881 ccagacttct atcatggcgt aaaaatcaaa aaattacctg ctttattgtg gtgataaaat
    26941 tcacgttcta caccagttcc agcatcgtta cttccgggcg gcagttcaga cgcaaaccat
    27001 acaaactgcc cacgccacgg gttgtgtaga tgtgtcgttc gccaaaggca tttaatccgg
    27061 cgacgtaacg tttatcttcg acaggggcaa aaggttcacc aaccaacggt acgcgcagtt
    27121 gcccgccgtg agtatggccg cacagcatca gatcccaggg ttcatcacgc atgacttctt
    27181 tgctgtcggg attatgtgcc agcatcagtc ttggcagatt tgcttcgctg gcgggaggtg
    27241 gtttgcattg tccggcccat aagtcaccgg tgcctaccag ttcgaattgc ctgttcggcg
    27301 tggcgatcac cgtagcctgg ttaaacaaca ccgtgatgcc cactgatttc aacgtctcgc
    27361 caattaagtg atttttttct gtaccaacag ggcgatcgtg gttgccgaag caggcaaacg
    27421 tcggcgcaca ttcggcaagg ggggagagga catcactaaa cgccgaaaaa ttcagcggca
    27481 tatcaaataa tacgtaatcg ccgcccagta atatcaaatc gggcttttgt tctatgccaa
    27541 gagcaatcgc gtcagaaatc aggcttaaag gaacaaaacg agagtaatgg agatcggcca
    27601 gaaaaagaat tttgaatggt gctgcgttgt ctttaaaaaa ggcgaggcgg tgacggatta
    27661 attcaaacca gcctggctcc cagtaatgca tataaccgaa acctgagctc gtggctatcg
    27721 tcgcggcagt agcctgcaac aatcggcggc gtgaaatcat cgctcatccc tgcaaaaaaa
    27781 atcgggcagc gtcgtgctgc ccgtgtgcat acttttagtc gatggtacgc agcagttcgt
    27841 taatgccgac tttgccgcga gttttcgcgt caactttctt aacgataacc gcacagtaga
    27901 ggctgtattt gccatctttt gacggcagat tacctgaaac aaccacagac cccgccggaa
    27961 cgtgaccgta gtggatttcg ccggtttcac ggtcgtaaat acgggtgctc tgaccaatgt
    28021 atacgcccat ggaaatgacg gaaccttctt cgacaatcac cccttcaact acttcagagc
    28081 gtgcgccaat gaagcagtta tcttcaatga tggttgggtt agcctgcagc ggttccagca
    28141 cgccgccgat gcccacgcca ccggaaaggt ggacgttttt accaatctgc gcacaagaac
    28201 cgacggtcgc ccaggtatca accatggtgc cttcatcaac atatgcgccg atgttgacgt
    28261 aagacggcat cagcacggtg ttacgggcaa taaacgcacc ctgacgtacc gccgctggtg
    28321 gcacaacgcg gaagccttct ttctggaaac gtgcttcgtc gtagtcggcg aatttcattg
    28381 gcactttgtc gaagtagcgg ctttctgccc cttcgatcac ctgattatca ttaatacgga
    28441 aagagagcag caccgctttt ttcaaccact gatgcgtcac ccactgaccg tcaatttttt
    28501 ccgctacacg cagtgcaccg gaatccagca gggcgatcac ctgatttacc gcttcgcggg
    28561 taacggtgtc tgcgttggtt ggcgtgatct cggcacggcg ttcaaaagcg gtttcaataa
    28621 tgttctgtaa ctgctgcatt gttaaactct tttcatatca gtaaacacat caccctttat
    28681 cgtttggatt gagggcctct gtcaaccgct gatgcacttc ctgctgcaac tcgttattaa
    28741 gcgcacgccg gtcagcggtg gcaattatga ataaatcttc tactcgctcg ccaatggttg
    28801 taattcgggc accatgaagc gaaattccca gatcggcaaa aattttcccg actctcgcca
    28861 gcagtcctgg ctggtcgagc gcgataagct caaggaacga tttgcggtcg gtatgggtcg
    28921 gcaaaaacgt tacttcggtt tcaacagtaa aatggcgtaa tttggcgggt tggcgacggg
    28981 gctgcggtgg ctgccagcta ctttgcgtca gtacttgctc cagaccaaac cgaataacct
    29041 catgacgatc tgcggacagc gggctgccat cgggttccag cacgataaag gtatccatcg
    29101 ccataccgtc gcgagtggtg aaaatttgtg cgtcgtgaac acttaaattg cggcggtcta
    29161 attcggcaca gacggcggca aacagatagg ggcggtccgg gctccagata aaaatctcgg
    29221 tgcctccacg cgtagcctgc gggctaagca ataccagtgg tttgcttaaa tcatgctgta
    29281 ataaatggcg ggcatgccag gccagttgat ttgggctatg gcggacaaaa tagttagcac
    29341 gacagcgtga ccaaatttgg tgcagcgcct cttcgtcgat gttatccatg cgcagtagtg
    29401 ccagtgcctg gagttggtga tggcgaaccc gttcgcgcat atccggcgtg ttttgcattc
    29461 cgcgtcgtag ctgcttttcg gtggcaaaat agagttcgcg caacagactt tgcttccagc
    29521 tattccacag cgtttcgttg gtggcgcaaa tgtcagccac tgtcaggcat accagatagc
    29581 gcagacgatt ttccgtttga acttcttcgg caaactgctt gatgacttca gggtcctgaa
    29641 tatcgcggcg ttgtgctgtc accgacatca gcaagtgctg gcgaaccagc caggcaacca
    29701 gctgtgtttc gcgtgagttc agcccgtgga gttctgcaaa atgcactaca tcctgagcac
    29761 cgagaatgga gtggtcgccg ccgcgtcctt tggcgatatc gtgaaacagc gcggcgatga
    29821 aaatcagctc agttgacggc aggcgcggcc agacgtccac acacaacgga tggcgctggc
    29881 gcgtttcttc actggcaaaa ctctccagtt tcagcatcac gcggatagta tgttcatcca
    29941 ccgtgtaggc gtggaacaga tcaaactgca tctgcccgac gatatgcgac cattgcggca
    30001 tatacgcgcc gagcacgcta tggcgatgca ttggcaatag cccgcgccgc accgctccgg
    30061 ggtgacgcag aatgctcaaa aacagttttc gtgcttccgg aatattacac agcggttgtt
    30121 gcagatggcg acgggcatgg cgtaactggc gcagcgtggt ggagtaaatg ccggtgatcg
    30181 cactgttgcg caccatggtg tagaacatac gcaagatggc ttccggctgg cgcataaata
    30241 gtgtttcatc acgcaggtcg attagcgtac cgcgtagctg aaactcatcg tcgattggac
    30301 gtggtttttc gtcggcagga agggcgagga tggcttcatc gaacagttgc agcagcatct
    30361 ggttgagttc actgacgcgg cgtgtaacgc ggaagtaatc cttcatcatc cgctcgaccg
    30421 gttcgttgcc ttcaccactg taattcagac gctgagcgac gctaagttgg cgatcgaata
    30481 acaggcgatt atcgtaacgg ctgacgacca gatgcagggc aaagcgaata cgccacagta
    30541 tatgcagaca ttcgtttaat tccgcccgct ccgctgaggt taagaagcca aacccaacca
    30601 tttcatccag cgatgttgcg ccaaaatgac ggcgggccac ccattgcaga gtgtggatat
    30661 cgcgcaagcc gccagggcta cttttgatgt ctggttcaag attgtagctg gtgccgtggt
    30721 aacgctgatg gcgctggttc tgttcttcaa ctttcgccgc gtagaacttg tcggaaggcc
    30781 agaatccttc gctaaaaata tgtttttgca gttcgaggaa gagcgcaaca tcgccaatta
    30841 ataagcggga ttcgattaaa ttggtggcga cggttaaatc cgataaccct tccagcatgc
    30901 actcttcaag cgtgcgcaca ctatgaccga cttccagctt tacatcccag agcagcgtta
    30961 acagctcgcc cactttttgc gcctgatcgt ccgggagctt tttacggctt aaaatcagta
    31021 aatcgatgtc agaaagtgga tgcagctcgc cacgaccgta gccaccgacg gcgaccaatg
    31081 ccaggtcggc aatctggctg aatcccgctt caatccataa tcgttgcagg agctggtcga
    31141 taaactcggt gcgcgcctca atcaactgtt ctgcagagat cccattgtca aaggcatcac
    31201 ccagccaacg ctggaaagta tcgatatggg cttttatccc accgacggtt aattcatcac
    31261 ggggccagac gcatggattt tgcggttgac cgggcagggt ggggagagcg gtgtttgcgt
    31321 actgttctgg aagggtattc attgtgcgcc actcataaga ttaaattatc gcattaaaaa
    31381 agccggcatt cgccggcttc atcttattcg tcgtgcgaga ttatcgccgg gatggtgtca
    31441 tccttgcgta gcgtcagaat ttcgcagccg ttatcagtca ccacaatagt atgctcatat
    31501 tgtgcagaca agctgcgatc tttggttttt accgtccagc catctttcat ggtgcggatc
    31561 tcttttttac ccgcgttgac cattggctcg atggtgaacg tcatcccagg tttcagtacg
    31621 acgttggttt cacgggagtc atagtgcagc acctgcggtt cttcatggaa gccgcgacca
    31681 ataccgtgtc cgcaatattc acgaacgacg gagaagcctt ctgcttcgac aaacttctga
    31741 atcgccgcac cgatttcgcg caggttaatg cctggtttga ccatgcgcag cgccaggtac
    31801 aggctttctt gcgtgatgcg gcacagacgt tcgcccatga tggtcggctt accgacgata
    31861 aacattttcg aggtatcgcc gtggaaacca tctttgatta cggtgacatc aatgttaacg
    31921 atatcgccat ctttcagcag cttcgcatcg tccgggatac cgtggcacac cacttcatta
    31981 atagagatgc aaacggattt cggatagccg tgatagccga ggcaggcaga aaccgcgtgt
    32041 tgttcattaa caatgtaatc attacagatg cgatccagct cgccggtgct gacgcccggt
    32101 ttaacatacg gttcgatcat ctccagcact tcggcagcca gtcggccagc gacgcgcatt
    32161 ttttcgatat cttctggggt cttgattgag atagccatta attctgtcca tcagcgtcgg
    32221 tgataccgac aatatatatg taagtgccgt caatggtatc acacccgggc aaattgagaa
    32281 tcattctgaa tttcgccaaa cgtgccactg aacgttttct ataatagaaa attcaacgtc
    32341 tgatgctgta cacagcgcca acaattattg gtgtccatga cgtatttgtg gtataaagcg
    32401 cgccggactt ccgatccatt tcgtatacac agactggacg gaagcgacaa tctcactttg
    32461 tgtaacaaca cacacgtatc ggcacatatt ccggggtgcc ctttggggtc ggtaatatgg
    32521 gatacgtgga ggcataaccc caacttttat atagaggttt taatcatggc aactgtttcc
    32581 atgcgcgaca tgctcaaggc tggtgttcac ttcggtcacc agacccgtta ctggaacccg
    32641 aaaatgaagc cgttcatctt cggtgcgcgt aacaaagttc acatcatcaa ccttgagaaa
    32701 actgtaccga tgttcaacga agctctggct gaactgaaca agattgcttc tcgcaaaggt
    32761 aaaatccttt tcgttggtac taaacgcgct gcaagcgaag cggtgaaaga cgctgctctg
    32821 agctgcgacc agttcttcgt gaaccatcgc tggctgggcg gtatgctgac taactggaaa
    32881 accgttcgtc agtccatcaa acgtctgaaa gacctggaaa ctcagtctca ggacggtact
    32941 ttcgaaaagc tgaccaagaa agaagcgctg atgcgcactc gtgagctgga gaaactggaa
    33001 aacagcctgg gcggtatcaa agacatgggc ggtctgccgg acgctctgtt tgtaatcgat
    33061 gctgaccacg aacacattgc tatcaaagaa gcaaacaacc tgggtattcc ggtatttgct
    33121 atcgttgata ccaactctga tccggacggt gttgacttcg ttatcccggg taacgacgac
    33181 gcaatccgtg ctgtgaccct gtacctgggc gctgtagctg caaccgtacg tgaaggccgt
    33241 tctcaggatc tggcttccca ggcggaagaa agcttcgtag aagctgagta ataaggcttg
    33301 ataactcccc caaaatagtt cgagttgcag aaaggcggca agctcgagaa ttcccgggag
    33361 cttacatcag taagtgaccg ggatgagcga gcgaagataa cgcatctgcg gcgcgaaata
    33421 tgaaggggga gagcccttat agaccaggta gtacacgttt ggttaggggg cctgcatatg
    33481 gccccctttt tcacttttat atctgtgcgg tttaatgccg ggcagatcac atctccgagg
    33541 attttagaat ggctgaaatt accgcatccc tggtaaaaga gctgcgtgag cgtactggcg
    33601 caggcatgat ggattgcaaa aaagcactga ctgaagctaa cggcgacatc gagctggcaa
    33661 tcgaaaacat gcgtaagtcc ggtgctatta aagcagcgaa aaaagcaggc aacgttgctg
    33721 ctgacggcgt gatcaaaacc aaaatcgacg gcaactacgg catcattctg gaagttaact
    33781 gccagactga cttcgttgca aaagacgctg gtttccaggc gttcgcagac aaagttctgg
    33841 acgcagctgt tgctggcaaa atcactgacg ttgaagttct gaaagcacag ttcgaagaag
    33901 aacgtgttgc gctggtagcg aaaattggtg aaaacatcaa cattcgccgc gttgctgcgc
    33961 tggaaggcga cgttctgggt tcttatcagc acggtgcgcg tatcggcgtt ctggttgctg
    34021 ctaaaggcgc tgacgaagag ctggttaaac acatcgctat gcacgttgct gcaagcaagc
    34081 cagaattcat caaaccagaa gacgtatccg ctgaagtggt agaaaaagaa taccaggtac
    34141 agctggatat agcgatgcag tctggtaagc cgaaagaaat cgcagagaaa atggttgaag
    34201 gccgcatgaa gaaattcacc ggcgaagttt ctctgaccgg tcagccgttc gttatggaac
    34261 caagcaaaac tgttggtcag ctgctgaaag agcataacgc tgaagtgact ggcttcatcc
    34321 gcttcgaagt gggtgaaggc atcgagaaag ttgagactga ctttgcagca gaagttgctg
    34381 cgatgtccaa gcagtcttaa ttatcaaaaa ggagccgcct gagggcggct tctttttgtg
    34441 cccatcttgt aaattcagct aacccttgtg gggctgcgct gaaaagcgac gtacaatgtc
    34501 gctagtatta attcatttca atcgttgaca gtctcaggaa agaaacatgg ctaccaatgc
    34561 aaaacccgtc tataaacgca ttctgcttaa gttgagtggc gaagctctgc agggcactga
    34621 aggcttcggt attgatgcaa gcatactgga tcgtatggct caggaaatca aagaactggt
    34681 tgaactgggt attcaggttg gtgtggtgat tggtgggggt aacctgttcc gtggcgctgg
    34741 tctggcgaaa gcgggtatga accgcgttgt gggcgaccac atggggatgc tggcgaccgt
    34801 aatgaacggc ctggcaatgc gtgatgcact gcaccgcgcc tatgtgaacg ctcgtctgat
    34861 gtccgctatt ccattgaatg gcgtgtgcga cagctacagc tgggcagaag ctatcagcct
    34921 gttgcgcaac aaccgtgtgg tgatcctctc cgccggtaca ggtaacccgt tctttaccac
    34981 cgactcagca gcttgcctgc gtggtatcga aattgaagcc gatgtggtgc tgaaagcaac
    35041 caaagttgac ggcgtgttta ccgctgatcc ggcgaaagat ccaaccgcaa ccatgtacga
    35101 gcaactgact tacagcgaag tgctggaaaa agagctgaaa gtcatggacc tggcggcctt
    35161 cacgctggct cgtgaccata aattaccgat tcgtgttttc aatatgaaca aaccgggtgc
    35221 gctgcgccgt gtggtaatgg gtgaaaaaga agggacttta atcacggaat aattcccgtg
    35281 atggataaat aagggtaaga ttccgcgtaa gtatcgcggg ggcgtaagtc tggttataag
    35341 gcgttattgt tgcaggcagt ttggtcacgg ccagcgcgca gcaaccggag cgtacaaaag
    35401 tacgtgagga tggcgagcac tgctcggggc caaaatggca aataaaatag cctaataatc
    35461 cagacgatta cccgtaatat gtttaatcag ggctatactt agcacacttc cactgtgtgt
    35521 gactgtctgg tctgactgag acaagttttc aaggattcgt aacgtgatta gcgatatcag
    35581 aaaagatgct gaagtacgca tggacaaatg cgtagaagcg ttcaaaaccc aaatcagcaa
    35641 aatacgcacg ggtcgtgctt ctcccagcct gctggatggc attgtcgtag aatattacgg
    35701 cacgccgacg ccgctgcgtc agctggcaag cgtaacggta gaagattccc gtactctgaa
    35761 aatcaacgtg tttgatcgtt caatgtctcc ggccgttgaa aaagcgatta tggcgtccga
    35821 tcttggcctg aacccgaact ctgcgggtag cgacatccgt gttccgctgc cgccgctgac
    35881 ggaagaacgt cgtaaagatc tgaccaaaat cgttcgtggt gaagcagaac aagcgcgtgt
    35941 tgcagtacgt aacgtgcgtc gtgacgcgaa cgacaaagtg aaagcactgt tgaaagataa
    36001 agagatcagc gaagacgacg atcgccgttc tcaggacgat gtacagaaac tgactgatgc
    36061 tgcaatcaag aaaattgaag cggcgctggc agacaaagaa gcagaactga tgcagttctg
    36121 atttcttgaa cgacaaaaac gccgctcagt agatccttgc ggatcggctg gcggcgtttt
    36181 gctttttatt ctgtctcaac tctggatgtt tcatgaagca actcaccatt ctgggctcga
    36241 ccggctcgat tggttgcagc acgctggacg tggtgcgcca taatcccgaa cacttccgcg
    36301 tagttgcgct ggtggcaggc aaaaatgtca ctcgcatggt agaacagtgc ctggaattct
    36361 ctccccgcta tgccgtaatg gacgatgaag cgagtgcgaa acttcttaaa acgatgctac
    36421 agcaacaggg tagccgcacc gaagtcttaa gtgggcaaca agccgcttgc gatatggcag
    36481 cgcttgagga tgttgatcag gtgatggcag ccattgttgg cgctgctggg ctgttaccta
    36541 cgcttgctgc gatccgcgcg ggtaaaacca ttttgctggc caataaagaa tcactggtta
    36601 cctgcggacg tctgtttatg gacgccgtaa agcagagcaa agcgcaattg ttaccggtcg
    36661 atagcgaaca taacgccatt tttcagagtt taccgcaacc tatccagcat aatctgggat
    36721 acgctgacct tgagcaaaat ggcgtggtgt ccattttact taccgggtct ggtggccctt
    36781 tccgtgagac gccattgcgc gatttggcaa caatgacgcc ggatcaagcc tgccgtcatc
    36841 cgaactggtc gatggggcgt aaaatttctg tcgattcggc taccatgatg aacaaaggtc
    36901 tggaatacat tgaagcgcgt tggctattta acgccagcgc cagccagatg gaagtgctga
    36961 ttcacccgca gtcagtgatt cactcaatgg tgcgctatca ggacggcagt gttctggcgc
    37021 agctggggga accggatatg cgtacgccaa ttgcccacac catggcatgg ccgaatcgcg
    37081 tgaactctgg cgtgaagccg ctcgattttt gcaaactaag tgcgttgaca tttgccgcac
    37141 cggattatga tcgttatcca tgcctgaaac tggcgatgga ggcgttcgaa caaggtcagg
    37201 cagcgacgac agcattgaat gccgcaaacg aaatcaccgt tgctgctttt cttgcgcaac
    37261 aaatccgctt tacggatatc gctgcgttga atttatcggt actggaaaaa atggatatgc
    37321 gcgaaccaca atgtgtggac gatgtgttat ctgttgatgc gaacgcgcgt gaagtcgcca
    37381 gaaaagaggt gatgcgtctc gcaagctgag gataatccgg ctacagagag tctcgctatt
    37441 tgttagcgta gggcttcagt gatatagtct gcgccatctg atcgtaagta gttggcttta
    37501 taaggtcagg tatgccgtgg ttttgcacgg ctttttttgt ataggcttca gtattcctga
    37561 gtaccgtaaa ccctgtcagg gaataaaaaa cgcgtgatgt tgtctgctac tcaactactt
    37621 agcgaaaaat tgccagcgca tggctgccgt catgttgcga tcattatgga cggcaatggt
    37681 cgctgggcaa aaaagcaagg gaagattcgt gcctttgggc ataaagccgg ggcaaaatcc
    37741 gtccgccggg ctgtctcttt tgcggccaac aacggtattg aggcgttaac gctgtatgcc
    37801 tttagtagtg aaaactggaa ccgaccagcg caggaagtca gtgcgttaat ggaactgttt
    37861 gtgtgggcgc tcgatagcga agtaaaaagt ctgcaccgac ataacgtgcg tctgcgtatt
    37921 attggcgata ccagtcgctt taactcgcgt ttgcaagaat gtattcgtaa atctgaagcg
    37981 ctaacagccg ggaataccgg tctgacgctg aatattgcgg cgaactacgg tggacgttgg
    38041 gatatagtcc agggagtcag gcaactggct gaaaaggtgc agcaaggaaa cctgcaacca
    38101 gatcagatag atgaagagat gctaaaccag catgtctgta tgcatgaact ggcccctgta
    38161 gatttagtaa ttaggactgg gggggagcat cgcattagta actttttgct ttggcaaatt
    38221 gcctatgccg aactttactt tatagatgtt ctctggcccg atttcgatga acaagacttt
    38281 gaaggggcgt taaatgcctt tgctaatcga gagcgtcgtt tcggcggcac cgagcccggt
    38341 gatgaaacag cctgatgggg ggcgcttttg ctgaagtatc gcctgatatc tgcttttgtg
    38401 ttaatacccg tcgtcatcgc ggcgttgttt ctgttgccgc cggtggggtt cgccattgta
    38461 acgctggtgg tctgcatgct ggcagcgtgg gaatggggac agcttagcgg ttttaccact
    38521 cgttcgcagc gagtatggtt ggcggtgtta tgcgggttat tgttggcgct gatgcttttt
    38581 ctgttgccgg aatatcaccg aaatattcat caaccgctgg ttgaaatctc actttgggct
    38641 tcgctgggtt ggtggattgt ctcgctattg ctggtgctgt tttacccagg ttccgcagca
    38701 atctggcgta actctaaaac attgcgcctt atttttggcg tgctaaccat tgttcccttc
    38761 ttctggggca tgctggcgtt acgggcctgg cactatgacg agaatcatta cagtggcgca
    38821 atatggctgc tctatgtcat gatcctggta tggggcgctg actccggcgc atatatgttt
    38881 ggcaaattgt ttggtaaaca taagctggca ccgaaggttt ctccgggtaa aacctggcaa
    38941 ggctttatag gtggactcgc tactgcagcg gtaatctcat ggggttatgg catgtgggcg
    39001 aatctcgacg tcgctcccgt caccttactc atttgctcta ttgtcgcagc gttagcctca
    39061 gtgctcggcg atctgaccga gagtatgttt aagcgtgaag caggaattaa ggacagcggt
    39121 catttaattc caggacacgg tggtatttta gatcgtattg atagcctgac ggctgcggta
    39181 ccggtctttg cttgcttgtt gttactggta ttcaggacgc tttaacggaa ggtaatatgc
    39241 tgagttttct ctgggatttg gcttcgttca tcgttgcact gggtgtactt atcactgtgc
    39301 atgaatttgg tcatttctgg gttgcccggc gttgtggtgt tcgcgttgag cgtttctcaa
    39361 tagggtttgg taaggcgctc tggcggcgaa ctgataagct cggcaccgaa tatgttatcg
    39421 ccctgatccc gctgggcggt tatgtcaaaa tgctggatga gcgcgcagaa ccggtcgttc
    39481 cggaactccg ccaccatgcc ttcaataata aatctgttgg ccaacgagcg gcgattattg
    39541 ccgcaggtcc ggttgcaaac ttcatttttg ctatctttgc ctactggctg gtttttatta
    39601 ttggtgtgcc tggcgtacgt ccggtggttg gtgaaatagc agccaattcg atagctgcgg
    39661 aagcacaaat tgcaccaggt acggaactaa aagccgtaga tggtatcgaa acgcctgatt
    39721 gggatgccgt gcgtttgcag ttggtcgata aaattggcga tgaaagcacc accattacag
    39781 tagcgccatt tggcagcgac caacggcggg atgtaaagct cgatttacgt cactgggcgt
    39841 ttgagcctga taaagaagat ccggtatctt cgctggggat tcgtcctcgt gggctgcaaa
    39901 ttgaacctgt actggaaaat gtgcagccaa actcggcggc aagcaaggca ggtttgcaag
    39961 caggcgacag gatcgttaaa gtcgatggtc agcccttaac gcagtgggtg acctttgtga
    40021 tgcttgtccg ggataacccg gggaaatcct tagcgttaga aatcgaaagg cagggaagtc
    40081 ccttgtcttt gacattaatc ccggagagta aaccgggtaa tggtaaagcg attggttttg
    40141 tcggtattga gccgaaagtc attcctttgc cagatgagta taaagttgta cgccagtatg
    40201 ggccgttcaa cgccatcgtc gaagccacgg acaaaacgtg gcagctgatg aagctgacgg
    40261 tcagtatgct gggaaaattg atcaccggtg atgtgaaact gaacaacctc agtgggccga
    40321 tctctatcgc caagggggct gggatgacag cggaactcgg ggtagtttat tacctgccgt
    40381 ttcttgcgct tattagcgtg aacttaggga taattaacct gtttccgttg cccgtacttg
    40441 acggggcgca tctgctgttc cttgcgatcg aaaagatcaa gggcggaccg gtatccgagc
    40501 gggttcaaga cttttgttat cgcattggct cgattctgct ggtgctgtta atggggcttg
    40561 cacttttcaa tgatttctct cggttatgag agttagttag gaagaacgca taataacgat
    40621 ggcgatgaaa aagttgctca tagcgtcgct gctgtttagc agcgccaccg tatacggtgc
    40681 tgaagggttc gtagtgaaag atattcattt cgaaggcctt cagcgtgtcg ccgttggtgc
    40741 ggccctcctc agtatgccgg tgcgtacagg cgacacggtt aatgatgaag atatcagtaa
    40801 taccattcgc gctctgtttg ctaccggcaa ctttgaggat gttcgcgtcc ttcgtgatgg
    40861 tgataccctt ctggttcagg taaaagaacg tccgaccatt gccagcatta ctttctccgg
    40921 taacaaatcg gtgaaagatg acatgctgaa gcaaaacctc gaggcttctg gtgtgcgtgt
    40981 gggcgaatcc ctcgatcgca ccaccattgc cgatatcgag aaaggtctgg aagacttcta
    41041 ctacagcgtc ggtaaatata gcgccagcgt aaaagctgtc gtgaccccgc tgccgcgcaa
    41101 ccgtgttgac ctaaaactgg tgttccagga aggtgtgtca gctgaaatcc agcaaattaa
    41161 cattgttggt aaccatgctt tcaccaccga cgaactgatc tctcatttcc aactgcgtga
    41221 cgaagtgccg tggtggaacg tggtaggcga tcgtaaatac cagaaacaga aactggcggg
    41281 cgaccttgaa accctgcgca gctactatct ggatcgcggt tatgcccgtt tcaacatcga
    41341 ctctacccag gtcagtctga cgccagataa aaaaggtatt tacgtcacgg tgaacattac
    41401 cgaaggcgat cagtacaagc tttctggcgt tgaagtgagc ggcaaccttg ccgggcactc
    41461 cgctgaaatt gagcagctga ctaagatcga gccgggcgag ctgtataacg gcaccaaagt
    41521 gaccaagatg gaagatgaca tcaaaaagct tctcggtcgc tatggttatg cctatccgcg
    41581 cgtacagtcg atgcctgaaa ttaacgatgc cgacaaaacc gttaaattac gcgtgaacgt
    41641 tgatgcgggt aaccgtttct acgtgcgtaa gatccgtttt gaaggtaacg atacctcgaa
    41701 agatgccgtc ctgcgtcgcg aaatgcgtca gatggaaggt gcatggctgg gaagcgatct
    41761 ggtcgatcag ggtaaggagc gtctgaatcg tctgggcttc tttgaaactg tcgataccga
    41821 tacccaacgt gttccgggta gcccggacca agtagatgta gtctacaagg tgaaagaacg
    41881 taacaccggt agcttcaact tcgggattgg ttacggtact gaaagtggcg tgagcttcca
    41941 ggctggcgtg caacaggata actggttagg tacaggttat gctgttggta tcaacgggac
    42001 caaaaacgat taccagacct atgctgaatt gtcggtaacc aacccgtact tcactgtaga
    42061 tggtgtaagc ctcggtggtc gtctcttcta taatgacttc caggcagatg acgccgacct
    42121 gtccgactat accaacaaga gttatggtac agacgtgacg ttgggcttcc cgatcaacga
    42181 atataactcg ctgcgtgcag gtctgggtta tgtacataac tccctgtcca acatgcagcc
    42241 tcaggttgcg atgtggcgtt atctgtactc tatgggtgaa catccgagca cctctgatca
    42301 ggataacagc ttcaaaacgg acgacttcac gttcaactat ggttggacct ataacaagct
    42361 tgaccgtggt tacttcccga cagatggttc acgtgtcaac ctgaccggta aagtgaccat
    42421 tcctggatcg gataacgaat actacaaagt gacgttagac acggcgactt atgtgccgat
    42481 cgatgacgat cacaaatggg ttgttctggg gcgtacccgc tggggttatg gtgatggttt
    42541 aggcggcaaa gagatgccgt tctacgagaa cttctatgcc ggtggttcca gcaccgtccg
    42601 tggcttccag tccaatacca ttggtccgaa agcagtttac ttcccgcatc aggccagtaa
    42661 ttatgatccg gactatgatt acgaatgtgc gactcaggac ggcgcgaaag acctgtgtaa
    42721 atcggatgat gctgtaggcg gtaacgccat ggcggttgcc agcctcgagt tcatcacccc
    42781 gacgccgttt attagcgata agtatgctaa ctcggttcgt acttccttct tctgggatat
    42841 gggtaccgtt tgggatacaa actgggattc cagccaatat tctggatatc cggactatag
    42901 tgatccaagc aatatccgta tgtctgcggg tatcgcatta caatggatgt ccccattggg
    42961 gccgttggtg ttctcctacg cccagccgtt caaaaagtac gatggagaca aggcagaaca
    43021 gttccagttt aacatcggta aaacctggta agtgttctcc acaaaggaat gtagtggtag
    43081 tgtagcgatg actttaggcg atcaatataa gatcgccggg ccacgcaaag aactgcaccc
    43141 tccggtgcaa atgggatggt aaggagttta ttgtgaaaaa gtggttatta gcggcaggtc
    43201 tcggtttagc actggcaact tctgctcagg cggctgacaa aattgcaatc gtcaatatgg
    43261 gcagcctgtt ccagcaggta gcgcagaaaa ccggtgtttc taacacgctg gaaaatgagt
    43321 tcaaaggccg tgccagcgaa ctgcagcgta tggaaaccga tctgcaggct aaaatgaaaa
    43381 agctgcagtc catgaaagcg ggcagcgatc gcactaagct ggaaaaagac gtgatggctc
    43441 agcgccagac ttttgctcag aaagcgcagg cttttgagca ggatcgcgca cgtcgttcca
    43501 acgaagaacg cggcaaactg gttactcgta tccagactgc tgtgaaatcc gttgccaaca
    43561 gccaggatat cgatctggtt gttgatgcaa acgccgttgc ttacaacagc agcgatgtaa
    43621 aagacatcac tgccgacgta ctgaaacagg ttaaataagt aatgccttca attcgactgg
    43681 ctgatttagc gcagcagttg gatgcagaac tacacggtga tggcgatatc gtcatcaccg
    43741 gcgttgcgtc catgcaatct gcacaaacag gtcacattac gttcatggtt aacccaaaat
    43801 accgtgagca tttaggcttg tgccaggcgt ccgcggttgt catgacccag gacgatcttc
    43861 ctttcgcgaa aagtgccgcg ctggtagtga agaatcccta cctgacttac gcgcgcatgg
    43921 cgcaaatttt agataccacg ccgcagcccg cgcagaacat tgcacccagt gcggtgatcg
    43981 acgcgacggc gaagctgggg aacaatgtat cgattggcgc taacgcggtg attgaatccg
    44041 gcgttgaact gggcgataac gtgattatcg gtgccggttg cttcgtaggt aaaaacagca
    44101 aaatcggtgc aggttcgcgt ctctgggcga acgtaaccat ttaccatgag atccagatcg
    44161 gtcagaattg cctgatccag tccggaacag tggtaggcgc agacggcttt ggttatgcca
    44221 acgatcgtgg taactgggtt aagatcccac agattggtcg cgtaattatt ggcgatcgcg
    44281 tggagatcgg tgcctgcact accatcgatc gcggcgcact ggatgacact gttattggca
    44341 atggcgtgat cattgataac cagtgccaga ttgcacataa cgtcgtgatt ggcgacaata
    44401 cggcggttgc cggtggcgtc attatggcgg gcagcctgaa aattggtcgt tactgcatga
    44461 tcggcggagc cagcgtaatc aacgggcata tggaaatatg cgacaaagtg acggttacgg
    44521 gcatgggtat ggtgatgcgt cccatcactg aaccaggcgt ctattcctca ggcattccgc
    44581 tgcaacccaa caaagtctgg cgcaaaaccg ctgcactggt gatgaacatt gatgacatga
    44641 gcaagcgtct gaaatcgctt gagcgcaagg ttaatcaaca agactaacgt tccatctttt
    44701 gttcgccaaa ctttacggcc tgtctcattc ttacgattgc ggcaggccgt gttattattg
    44761 tcgtttctta tattttgaca ggaagagtat cttgactact aacactcata ctctgcagat
    44821 tgaagagatt ttagaacttc tgccgcaccg tttcccgttc ttactggtgg atcgcgtgct
    44881 ggattttgaa gaaggtcgtt ttctgcgcgc agtaaaaaat gtctctgtca atgagccatt
    44941 cttccagggc catttccctg gaaaaccgat tttcccgggt gtgctgattc tggaagcaat
    45001 ggcacaggca acaggtattc tggcgtttaa aagcgtagga aaactggaac cgggtgagct
    45061 gtactacttc gctggtattg acgaagcgcg cttcaagcgc ccggtcgtgc ctggcgatca
    45121 aatgatcatg gaagtcactt tcgaaaaaac gcgccgcggc ctgacccgtt ttaaaggggt
    45181 tgctctggtc gatggtaaag tagtttgcga agcaacgatg atgtgtgctc gtagccggga
    45241 ggcctgatac gtgattgata aatccgcctt tgtgcatcca accgccattg tggaagaggg
    45301 cgcgtcaatt ggcgcgaacg cacacattgg tcctttttgt atcgttggac cccatgtcgg
    45361 aattggtgag ggtaccgtac tgaaatctca cgttgtcgtg aatggtcata ctaaaattgg
    45421 ccgcgataat gagatttatc agttcgcctc catcggcgaa gttaaccagg atctgaaata
    45481 tgctggcgaa ccgacccgtg tggaaatcgg cgatcgtaac cgcattcgcg aaagcgtcac
    45541 cattcatcgt ggcacagtcc agggcggtgg attgacgaag gtgggcagcg acaacttact
    45601 gatgatcaac gcgcacattg cgcacgattg tacggtaggt aaccgctgta ttctcgccaa
    45661 caacgcaacg ctggcgggtc acgtatcggt tgacgacttc gcgatcatcg gcggcatgac
    45721 cgcagtccat cagttctgca tcattggtgc gcacgtgatg gttggcggct gctccggtgt
    45781 ggcgcaggac gtccctcctt atgtcattgc gcagggtaac cacgcaacgc cgttcggtgt
    45841 caatatcgaa gggctgaagc gccgcggatt cagccgtgag gcgattaccg ctatccgcaa
    45901 tgcgtataag ctgatttatc gtagcggtaa aacgctcgat gaagtgaaac cggaaattgc
    45961 tgaactggcg gaaacatatc cggaagtgaa agcctttacc gatttctttt cacgctcaac
    46021 gcgcggtctg attcgttaat gactgaacag cgtccattaa cgattgccct ggtcgccgga
    46081 gaaacctccg gcgatatcct gggggccggt ttaatccgcg ctctgaaaga acgtgtgccc
    46141 aacgcccgct ttgttggtgt tgccgggcca cgaatgcagg ctgaaggctg cgaagcctgg
    46201 tacgaaatgg aagaactggc ggtgatgggc attgttgaag tgctcggtcg tctgcgtcgc
    46261 ttactgcata ttcgtgccga tctgacaaag cgttttggcg aactgaagcc agatgttttt
    46321 gttggtattg atgcgcctga cttcaatatt actcttgaag gtaacctcaa aaagcagggt
    46381 atcaaaacca ttcattacgt cagtccgtca gtctgggcgt ggcgacagaa acgtgttttc
    46441 aaaataggca gagccaccga tctggtgctc gcatttctgc ctttcgaaaa agcgttttat
    46501 gacaaataca acgtaccgtg ccgctttatc ggtcatacca tggctgatgc catgccatta
    46561 gatccagata aaaatgccgc ccgtgatgtg ctggggatcc ctcacgatgc ccactgcctg
    46621 gcgttgctac cggggagccg tggtgcagaa gttgaaatgc ttagtgccga tttcctgaaa
    46681 acggcccagc ttttgcgcca gacatatccg gatctcgaaa tcgtggtgcc actggtgaat
    46741 gccaaacgcc gcgagcagtt tgaacgcatc aaagctgaag tcgcgcgaga cttttcagtt
    46801 catttgctgg atgggatggg ccgtgaggcg atggtcgcca gcgatgcggc gctactggcg
    46861 tcgggtacgg cagccctgga gtgtatgctg gcgaaatgcc cgatggtggt gggatatcgc
    46921 atgaagcctt ttaccttctg gttggcgaag cggctggtga aaactgatta tgtctcgctg
    46981 ccaaatctgc tggcgggcag agagttagtc aaagagttat tgcaggaaga gtgtgagccg
    47041 caaaaactgg ctgcggcgct gttaccgctg ttggcgaacg ggaaaatcag ccacgcgatg
    47101 cacgatacct tccgtgaact gcatcagcag atccgctgca atgccgatga gcaggcggca
    47161 caagccgttc tggagttagc acaatgatcg aatttgttta tccgcacacg cagctggttg
    47221 cgggtgtgga tgaagtcgga cgcgggccgt tagttggcgc ggtcgtcacc gctgcggtga
    47281 tccttgaccc ggcgcgcccg attgccgggc tgaatgattc caaaaagctg agcgaaaaac
    47341 gccgtctggc gctctgtgaa gagatcaaag agaaagcgtt gagctggagt ctgggccgcg
    47401 cggaacccca cgaaatcgac gagctgaaca ttcttcatgc gaccatgctg gcgatgcagc
    47461 gtgccgtcgc tgggctgcat attgcgccgg aatatgtgtt gattgatggt aaccgctgcc
    47521 cgaaattacc gatgcctgcg atggctgtgg tgaaaggcga tagccgcgta ccggaaatca
    47581 gtgccgcgtc tatcctggcg aaagtgacgc gtgacgccga aatggcggcg ctggatattg
    47641 ttttcccgca atatggtttt gcccaacaca aagggtaccc aaccgctttt catctggaaa
    47701 aactggctga acacggcgcg accgaacacc atcggcgcag ctttgggcct gtcaaacgcg
    47761 cactgggact tgcgtcctga ttcttgtgtc gagattaagt aaaccggaat ctgaagatgt
    47821 ctgaaccacg tttcgtacac ctgcgggtgc acagcgacta ctcgatgatc gatggcctgg
    47881 ccaaaactgc accgttggta aaaaaggcgg cggcgttggg tatgccagca ctggcgatca
    47941 ccgatttcac caacctttgt ggtctggtga agttctacgg agcgggacat ggcgcaggga
    48001 ttaagcctat cgtcggggca gattttaacg tccagtgcga cctgctgggt gatgagttaa
    48061 cccacctgac ggtactggcg gcgaacaata ccggctatca gaatctgacg ttgctgatct
    48121 caaaagcgta tcagcgcggg tacggtgccg ctgggccgat catcgatcgc gactggctta
    48181 tcgaattaaa tgaagggttg atccttcttt ccggcgggcg catgggcgac gtcggacgca
    48241 gtcttttgcg tggtaacagc gcgctggtag atgagtgtgt cgcgttttat gaagaacact
    48301 tcccggatcg ctattttctc gagctgatcc gcaccggcag gccggatgaa gaaagctatc
    48361 tgcacgcggc ggtggaactg gcggaagcgc gcggtttgcc cgtcgtggcg actaacgatg
    48421 tgcgctttat cgacagcagc gactttgacg cacacgaaat ccgcgtcgcg atccacgacg
    48481 gctttaccct cgacgatcct aaacgcccgc gtaactattc gccgcagcaa tatatgcgta
    48541 gcgaagagga gatgtgtgag ctgtttgccg acatccccga agcccttgcc aacaccgttg
    48601 agatcgccaa acgctgtaac gtaactgtgc gtcttggtga atacttcctg ccgcagttcc
    48661 cgaccgggga catgagcacc gaagattatc tggtcaagcg tgcaaaagag ggcctggaag
    48721 agcgtctggc ctttttattc cctgacgagg aagaacgtgt taagcgccgc ccggaatatg
    48781 acgaacgtct ggagactgaa cttcaggtta tcaaccagat gggcttcccg ggctacttcc
    48841 tcatcgttat ggaatttatc cagtggtcga aagataacgg cgtaccggta gggccaggcc
    48901 gtggctccgg tgcgggttca ctggtggcct acgcgctgaa aatcaccgac ctcgatccgc
    48961 tggaatttga cctgctgttc gaacgtttcc ttaacccgga acgtgtctcc atgcctgact
    49021 tcgacgttga cttctgtatg gagaaacgcg atcaggttat cgagcacgta gcggacatgt
    49081 acggtcgtga tgcggtatcg cagatcatca ccttcggtac aatggcggcg aaagcggtga
    49141 tccgcgacgt aggccgcgtg ctggggcatc cgtacggctt tgtcgatcgt atctcgaaac
    49201 tgatcccgcc cgatccgggg atgacgctgg cgaaagcgtt tgaagccgag ccgcagctgc
    49261 cggaaatcta cgaagcggat gaagaagtta aggcgctgat cgacatggcg cgcaaactgg
    49321 aaggggtcac ccgtaacgcc ggtaagcacg ccggtggggt ggttatcgcg ccgaccaaaa
    49381 ttaccgattt tgcgccgctt tactgcgatg aagagggcaa acatccggtc acccagtttg
    49441 ataaaagcga cgttgaatac gccggactgg tgaagttcga cttccttggt ttgcgtacgc
    49501 tcaccatcat caactgggcg ctggagatga tcaacaagcg gcgggcgaag aatggcgagc
    49561 cgccgctgga tatcgccgcg atcccgctgg atgacaagaa aagcttcgac atgctgcaac
    49621 gctcggaaac cacggcggta ttccagcttg aatcgcgcgg catgaaggat ctgatcaagc
    49681 gtctgcaacc tgactgcttc gaagatatga tcgcactggt ggcgctgttc cgcccaggtc
    49741 cgttgcaatc agggatggtg gataacttta tcgaccgtaa acatggtcgc gaagaaatct
    49801 cctatccgga cgtacagtgg cagcatgaaa gcctgaaacc ggtactggag ccaacctacg
    49861 gcatcatcct gtatcaggaa caggtcatgc agattgcgca ggtgctttct ggttataccc
    49921 tcggtggcgc ggatatgctg cgtcgtgcga tgggtaagaa aaagccggaa gagatggcta
    49981 agcagcgttc tgtatttgct gaaggtgcag aaaagaacgg agtcaacgcc gaactggcga
    50041 tgaaaatctt cgacctggtg gagaaattcg cgggttacgg atttaacaaa tcgcactctg
    50101 cggcctatgc tttggtgtca tatcaaacgt tatggctgaa agcgcactat ccggcggagt
    50161 ttatggcggc ggtaatgacc gccgatatgg acaacaccga gaaggtggtg ggcctggtgg
    50221 atgagtgctg gcggatgggg ctgaaaatcc tgccaccaga tataaactcc ggtctttacc
    50281 atttccacgt caacgacgac ggcgaaatcg tgtatggtat tggcgccatc aaaggggtag
    50341 gtgaaggtcc gattgaggcc atcatcgaag cccgtaataa aggcggctac ttccgcgaac
    50401 tgtttgatct ctgcgcccgt accgacacca aaaagttaaa ccggcgggtg ctggaaaaac
    50461 tgatcatgtc cggggcgttt gaccgtcttg ggccacaccg cgcggcgctg atgaactcgc
    50521 tgggtgatgc gttaaaagcg gcagatcaac acgcgaaagc ggaagctatc ggtcaggccg
    50581 atatgttcgg cgtgctggcc gaagagccgg aacaaattga acaatcctac gccagctgcc
    50641 aaccgtggcc ggagcaggtg gtgttagatg gggaacgtga aacgttaggt ctgtacctta
    50701 cgggacaccc tatcaaccag tatttaaaag agattgagcg ttatgtcgga ggcgtaaggc
    50761 tgaaagacat gcacccgaca gaacgtggta aagtcatcac ggctgcgggg ctcgttgttg
    50821 ccgcgcgggt tatggtcacc aagcgcggca atcgtatcgg tatctgcacg ctggatgacc
    50881 gttccgggcg gctggaagtg atgttgttta ctgacgccct ggataaatac cagcaattgc
    50941 tggaaaaaga ccgcatactt atcgtcagcg gacaggtcag ctttgatgac ttcagcggtg
    51001 ggcttaaaat gaccgctcgc gaagtgatgg atattgacga agcccgggaa aaatatgcta
    51061 gcgggcttgc tatctcgctg acggacaggc aaattgatga ccagctttta aaccgactcc
    51121 gtcagtctct ggaaccccac cgctctggga caattccagt acatctctac tatcagaggg
    51181 cggatgcacg cgcgcggttg cgttttggcg cgacgtggcg tgtctctccg agcgatcgtt
    51241 tattaaacga tctccgtggc ctcattggtt cggagcaggt ggaactggag tttgactaat
    51301 acaggaatac tatgagtctg aatttccttg attttgaaca gccgattgca gagctggaag
    51361 cgaaaatcga ttctctgact gcggttagcc gtcaggatga gaaactggat attaacatcg
    51421 atgaagaagt gcatcgtctg cgtgaaaaaa gcgtagaact gacacgtaaa atcttcgccg
    51481 atctcggtgc atggcagatt gcgcaactgg cacgccatcc acagcgtcct tataccctgg
    51541 attacgttcg cctggcattt gatgaatttg acgaactggc tggcgaccgc gcgtatgcag
    51601 acgataaagc tatcgtcggt ggtatcgccc gtcttgacgg tcgtccggtg atgatcattg
    51661 gtcatcaaaa aggtcgtgaa accaaagaaa aaattcgccg taactttggt atgccagcgc
    51721 cagaaggtta ccgcaaagca ctgcgtctga tgcaaatggc tgaacgcttt aagatgccta
    51781 tcatcacctt tatcgacacc ccgggggctt atcctggcgt gggcgcagaa gagcgtggtc
    51841 agtctgaagc cattgcacgc aacctgcgtg aaatgtctcg cctcggcgta ccggtagttt
    51901 gtacggttat cggtgaaggt ggttctggcg gtgcgctggc gattggcgtg ggcgataaag
    51961 tgaatatgct gcaatacagc acctattccg ttatctcgcc ggaaggttgt gcgtccattc
    52021 tgtggaagag cgccgacaaa gcgccgctgg cggctgaagc gatgggtatc atcgctccgc
    52081 gtctgaaaga gctgaaactg atcgactcca tcatcccgga accgctgggc ggcgctcacc
    52141 gtaacccgga agcgatggcg gcatcgttga aagcgcaact gctggcggat ctggccgatc
    52201 tcgatgtgtt aagcactgaa gatttaaaaa atcgtcgtta tcagcgcctg atgagctacg
    52261 gttacgcgta attcgcaaaa gttctgaaaa agggtcactt cggtggccct tttttatcgc
    52321 cacggtttga gcaggttatg attaaggaag gattttccag gaggaacaca tgaacatcat
    52381 tgccattatg ggaccgcatg gcgtctttta taaagatgag cccatcaaag aactggagtc
    52441 ggcgctggtg gcgcaaggct ttcagattat ctggccacaa aacagtgttg atttgctgaa
    52501 atttatcgaa cataaccctc gaatttgcgg cgtgattttt gactgggatg agtacagtct
    52561 cgatttatgc agcgatatca atcaacttaa tgaatatctc ccgctttatg ccttcatcaa
    52621 cacccactcg acgatggatg tcagcgtgca ggatatgcgg atggcgctct ggttttttga
    52681 atatgcgctg gggcaggtgg aagatatcgc cattcgtatg cgtcagtaca ccaacgaata
    52741 tcttgataac attacgccgc cgttcacgaa agccttgttt acctacgtta aagagcggaa
    52801 gtacaccttt tgtacgccgg ggcatatggg cggcaccgca tatcaaaaaa gcccggttgg
    52861 ctgcctgttt tatgattttt tcggcgggaa taccctcaag gctgatgtct ctatttcggt
    52921 caccgagctt ggttcgttgc tcgaccacac cgggccacac ctggaagcgg aagagtacat
    52981 cgcgcggact tttggcgcgg aacagagtta tatcgttacc aacggaacat cgacgtcgaa
    53041 caaaattgtg ggtatgtacg ccgcgccatc cggcagtacg ctgttgatcg accgcaattg
    53101 tcataaatcg ctggcgcatc tgttgatgat gaacgatgta gtgccagtct ggctgaaacc
    53161 gacgcgtaat gcgttgggga ttcttggtgg gatcccgcac cgtgaattta ctcgcgacag
    53221 catcgaagag aaagtcgctg ccaccacgca ggcacaatgg ccggttcatg cggtgattac
    53281 caactccacc tatgatggct tgctctacaa caccgactgg atcaaacaga cgctggatgt
    53341 cccgtcgatc cacttcgatt ccgcctgggt gccgtacacc cattttcatc cgatctacca
    53401 ggggaaaagt gatcttcgaa acgcaatcga cccacaaaat gctggcggcg ttatcgcagg
    53461 cttcgctgat ccacattaaa ggcgagtatg acgaagaggc gtttaacgaa gcctttatga
    53521 tgcataccac cacctcgccc agttatccca ttgttgcttc ggttgagacg gcggcggcga
    53581 cgctgcgtgg taatccgggc aaacggctga ttaaccgttc ggtggagcgg gcgctgcatt
    53641 ttcgcaaaga ggtccagcgg ttgcgggaag agtctgacgg ttggtttttc gatatctggc
    53701 aaccgccgca ggtggatgaa gccgaatgct ggcccgttgc gccaggcgaa cagtggcacg
    53761 gctttaacga tgcggatgcc gatcatatgt ttctcgatcc ggttaaagtc actattttga
    53821 taccggggat ggacgaacag ggcaatatga gcgaggaggg gatcctggcg gcgctggtgg
    53881 caaaattcct cgacgaacgt gggatcgtag tagagaaaac cggcccttat aacctgctgt
    53941 ttctctttag tattggtatc gataaaacca aagcaatggg attattgcgt gggttgacgg
    54001 aatttaaacg ctcttacgat ctcaacctgc ggatcaaaaa tatgctaccc gatctctatg
    54061 ctgaagatcc cgatttctac cgcaatatgc gtattcagga tctggcgcaa gggatccata
    54121 agctgattcg taaacacgat cttcccggtt tgatgttgtg ggcattcgat actttgccgg
    54181 aaattatcat gacgccacat caggcatggc agagacagat taaaggtgaa gtagaaacca
    54241 ttgcgctgga acaactggtc ggtagagtat cggcaaatat gatcctgcct tatccaccgg
    54301 gcgtaccgct gttgatgcca ggagaaatgc tgaccaaaga gagccgcacg gtactcgatt
    54361 ttctactgat gctttgttcc gtagggcaac attaccccgg ttttgaaacg gatattcacg
    54421 gcgcgaaaca ggacgaagac ggcgtttacc gcgtacgagt cctaaaaatg gcgggataac
    54481 ttgccagagc ggcttacggg cgagtaacgt gctgttaaca aataaaggag acgttatgct
    54541 gggtttaaaa caggttcacc atattgcgat tattgcgacg gattatgcgg tgagcaaagc
    54601 tttctactgc gatattcttg gtttcacgct gcaaagcgaa gtctatcgcg aagcgcgcga
    54661 ctcgtggaaa ggggatttgg cgcttaatgg gcaatatgtg attgagcttt tctcttttcc
    54721 gttcccgccg gagcgcccca gccgaccgga agcttgcggc ctgcgtcatc tggcttttag
    54781 cgttgatgac atcgatgcgg cagtggcgca ccttgaaagc cataacgtga agtgtgaagc
    54841 catccgtgtc gatccataca cgcaaaaacg cttcaccttc tttaacgatc cggacgggct
    54901 gccgttggaa ctgtatgagc agtaaggctt gtcatcgccg catttgcccg gtaacgtgcc
    54961 gggcattgct actgtaaaat cgcaccatca tgacactcac gctcaataga caacttctca
    55021 cctcacgcca gattttggtc gcctttagcg gcgggcttga ctccaccgtt ctgctgcatc
    55081 agttggtgca gtggcggacg gaaaatccgg gcgtcactct gcgcgctatt catgtgcatc
    55141 acggtttaag tgccaatgcc gatgcctggg ttacgcattg cgaaaacgtc tgccaacagt
    55201 ggcaggtgcc gctggtggtc gaacgcgtac aacttgcgca agaggggctg gggattgagg
    55261 cccaggcgcg gcaggcgcgt tatcaggcat ttgcccgtac cctattgccc ggcgaagtgc
    55321 tggtcaccgc gcaacatctc gacgatcaat gtgaaacctt tctgctggcg ctaaaacgcg
    55381 gtagcggccc tgccgggctt tcggctatgg cggaagtttc ggagtttgcc ggaacacggc
    55441 ttatccgccc gttgcttgcc cgcacgcggg gggaactggc gcagtgggca ttggcgcatg
    55501 gtttacgctg gattgaagac gaaagtaatc aggacgactc atacgatcgt aactttctgc
    55561 gcctgcgcgt agtgccgtta ttgcagcagc gttggccgca ttttgcggaa gcaacggccc
    55621 gcagcgccgc actttgtgct gaacaagaga gtctgttgga tgaactgctg gcggatgatt
    55681 tagcacactg ccaaacgtcg caggggacgc tgcagattgc gccaatgctg gcgatgagtg
    55741 atgcccgccg cgcggcgatt atccgtcgct ggctggcagg gcagaatgca ccaatgcctt
    55801 cccgcgacgc gctggtgcgg atctggcagg aagtggcgct ggcgcgggaa gatgcctccc
    55861 cctgtttacg cttgggcgcg tttgaaatcc gccgctatca gtcgcaactg tggtggatta
    55921 aatccgtcac cgggcaaagt gaaaccattg tgccgtggca gacgtggcta caaccgctgg
    55981 aattaccggc ggggctggga agcgtacagc ttactgcggg aggcgatatt cgccctccgc
    56041 gtgcagacga agcggtcagc gtgcgtttca aatcggcagg attgctgcat attgtcgggc
    56101 gcaacggcgg acgtaagctg aagaaaatct ggcaagagtt aggcgtgccg ccgtggctac
    56161 gtgacaccac gccactgctg ttttatggcg aaacgctgat tgcggcggca ggggtatttg
    56221 tgacgcaaga aggtgtggct gaaggtgaga atggcgtaag ttttgtctgg cagaaaacgc
    56281 ttagttaagt gaaagccgga taagacgcat caaacgtcgc atccggcgaa agtcaatcag
    56341 gactcgctca ccaccaccgt accgatttcc gggtggctaa agctggtaat tttatccaga
    56401 cgtagctcac gggtttcgcc agcggcctcg acgaccagat actccacatt tttgcgggag
    56461 actaaatcgc tggcttttgc ctgcaatttt tcgccatctt tcagctcaag tgtcagcatt
    56521 aaatgatgct ggcaggcgag ctcaagatta tcgtaatcat cacaattgat tggttgatac
    56581 gtatcattca ttaacataat cgctcaccag taagtttgcc gcagcgtatg ctgctttttc
    56641 cctgacagcc tcagaaaggg cgtcgtcggc agccatttca ttcagcactt tcaaaacgca
    56701 gcccagcgcg tccggaacgt atcctaagtc tccgctggcg atttccgcgt accgcttgcg
    56761 tattaactca caatattttt ccacatgccc tcctgtcagc acactgactt aaccgtggat
    56821 gcaagtctaa gcctacgaag ataaactctg tttcgcaagg tgactatacc acactcattt
    56881 ctgcaatatc agcgccgcaa ctgcacgtat tccgttacaa tggcctcctg attcgaaagg
    56941 agttttctta tggcgcttaa agcgacaatt tataaagcga cggttaatgt ggccgatctc
    57001 gaccgcaacc agtttctcga tgcctctctg acgctggcgc gccacccttc agaaacccag
    57061 gagcgtatga tgctgcgcct gctggcgtgg ctgaaatatg ctgatgaacg tctgcaattt
    57121 acccgtggtt tgtgtgccga tgatgagccg gaagcgtggc tgcgtaacga tcacctgggc
    57181 attgatttgt ggattgagct ggggctgccg gatgagcggc ggattaagaa agcctgcacc
    57241 caggccgcag aagtggcgct gtttacctat aatagtcggg cggcgcaaat ctggtggcag
    57301 caaaatcaga gcaaatgtgt gcagtttgcc aatctttccg tctggtatct ggacgatgaa
    57361 caactggcga aagtaagcgc ctttgccgat cgtaccatga cgctgcaggc aacgattcag
    57421 gatggcgtga tctggttatc ggacgataag aataatctgg aagttaactt aaccgcctgg
    57481 caacaacctt catgattgtg atttcccgac atgttgctat tcccgatggt gagcttgaga
    57541 tcaccgccat tcgtgcgcag ggcgcgggcg ggcagcatgt taataagacc tcaacggcca
    57601 ttcatctgcg ttttgacatt cgggcgtcca gcctgccaga gtattacaaa gagcgtctgc
    57661 tcgccgccag ccatcatttg atcagcagtg atggcgtgat tgtcattaag gcacaggaat
    57721 accgcagtca ggaactgaac cgcgaagccg cgctagcccg gctggtggca gtgattaaag
    57781 atttaacaac agaacaaaaa gcccgacgac ccacgcggcc cacccgtgca tcgaaagagc
    57841 gcaggctggc atcgaaagca caaaaatcaa gcgcgaaggc gatgcgcggc aaagtacgca
    57901 gcggtcggga ataaaaagaa ggaatagatg gtgaaaaaag cgatagtgac agcgatggct
    57961 gtaatcagcc tctttactct gatgggatgt aataaccggg ccgaagtcga tacgctttct
    58021 ccagcacagg ctgccgaact gaaaccgatg ccgcaaagtt ggcgcggcgt gctgccgtgt
    58081 gctgattgtg aaggaatcga aacctctctg ttcctcgaaa aagacggaac atgggtgatg
    58141 aatgagcgtt atctcggtgc tcgtgaagaa ccttcctcct tcgcttccta cggtacatgg
    58201 gcgcgaaccg ctgacaagct ggtattaacc gacagcaaag gtgaaaagtc atattatcgg
    58261 gcgaaaggcg atgcgctgga gatgctcgat cgtgaaggca atccgattga atcgcagttc
    58321 aactatacgc tggaaccggc acaatccagt ttacctatga cgccgatgac cctgcgaggc
    58381 atgtattttt atatggctga tgcggcgacc ttcactgatt gcgcgaccgg aaaacgtttc
    58441 atggtagcga ataacgcaga gctggagcgt ggctacctgg ctgcgcgcgg tcacagtgaa
    58501 aaaccggtgt tactgtcagt agaaggtcac tttacgcttg aggctaatcc ggatactggt
    58561 gcgccaacca aagtattagc gcccgatacg gcgggtaaat tttacccgaa taaggattgc
    58621 agtagtttgg ggcagtaaac ctgcttaagg cagaaacaaa cgcaaaacag gccagaagga
    58681 tatatttcaa cattttgaat ttgcacgttt tttgtaggcc ggataaggcg tttacgccgc
    58741 atccggcaat ggtgctcaac gcctgatgcg acgctggcgc gtcttatcat gcctacaacc
    58801 cccctcatac cttaaggcgg ataaggcatt tactttatca ccgggtcaaa ccaacaaagc
    58861 gacccgcctt gatataaatc cctggcttca ggtgcccaac atactgtaac tgcgtttctg
    58921 gcttaaacgc agagacatca ccaatacgca tatgcatcag atcggcaggg ctaatccagc
    58981 ccgattgcgc cagtgtaagt ggatgtccgg ctttggcaaa tgcatcagtg acaaattccg
    59041 aacaaaacca cgactttttg tctccttcgc ccacactgct taactgcgct ttcgccaggc
    59101 cgctgacgca ctgctggcga aaatcctcgg agaactgatt cagtgagcac atctggcgag
    59161 tcaccataaa gggaataaat tcgacaatgc cgcgatagtt ataaccgcta tctttgattt
    59221 tattggcaaa cgcggtgata tctgtggctt gttgcggggt aagatccggg actcgtaaga
    59281 cgaaaagctt atcactatgc ttcatcgctt ttttaaggga aacaatctgg acgccagcgc
    59341 ctgtcgcttc tgcaacgtta ttatcaccca gaaagattgc aacgtgactc acagaggaag
    59401 tgctgaagac gcggattcca aatgaggtta ccccaaggct tgaggagaac agcaaatcgc
    59461 cgggtttgag atcaggtgcc gttatttctt ttattgattg ttcggtgaaa gagctttgat
    59521 gctggaattt gacagcccat gtttttgcct cggcatctac tgctgtggct gatggggctg
    59581 gctgatatca acggtacaag ccgaaagtaa aagaaagctg gggagaagca ggcggcagta
    59641 cgcctttggt ttatccattt tatacaatcc atgtaaaaaa gggccctgaa attcaggacc
    59701 ctttctggca tcagccttta atctgtttca ccagatattc gacgatgtca ccagtcttaa
    59761 ttaactgttt ctcgccgttg cgacgatatt tatattcgat atcgtcgttg tcgaggttac
    59821 ggtcgcccag cacaatagtg tgcggaatac cgatcagttc catatcagca aacatcacgc
    59881 ccggacgctc tttgcggtca tccagcagca cttcgatacc ttgtgcacgc agttcgctgt
    59941 acagtttctc agcaagctct tgtacgcgga aggatttgtg catgttcatc ggcagaatcg
    60001 ccacctggaa cggcgcgata gcgtcaggcc atacgatgcc gcgttcgtcg tagttctgct
    60061 caatcgccgc agctaccaca cgcgttaccc cgataccgta gcaacccatc gtcaggattt
    60121 ggttacggcc atcttcaccc tgtacggagg ctttcagtgc ttcggagtac ttggtaccca
    60181 gctggaagat gtgaccaact tcgataccac gtttgatcag cagcgtaccc tggccatccg
    60241 ggcttggatc gccagccacc acgttacgga tatctgcaac ttccggggta gcgacatcgc
    60301 gatcccagtt gataccgaag tagtgtttac catcgatgtt agcaccagca gcgaaatcac
    60361 tcatcgccgc aacggtacgg tcaatcacca ccggaatcgg catgtttacc ggacccagtg
    60421 aacccggacc ggctttaacc acagcacgaa tttcttcttc ggtcgcgaaa gtcagcgggc
    60481 ttgcaacctg cggcagtttt tctgctttaa cttcgttcag ctcgtgatca ccgcgcacca
    60541 gcagcgcaac cagcgggaag ctgctgcctt caaccgcttt aaccagcaga gtcttaaccg
    60601 ttttctcaat cggcagattg aactgttcaa ccagttccgc gatggttttc gcgttcggcg
    60661 tatcaaccag cgtcatttcc tgggtagcag cagcgcgcgg ttctttcggc gcgatagctt
    60721 ctgccagttc aatgttcgct gcatagtcag aggtgtcgga gaagaccaca tcgtcttcac
    60781 cgctctgcgc cagcacctgg aattcgtgag aggcgctgcc gccgatagaa ccggtgtcgg
    60841 cttgcacggc gcggaaatcc agccccatgc ggctgaagat tttgctgtag gccgcataca
    60901 ttgcatcgta ggtttcctgc agggattcct gagaagtatg gaaagagtaa gcatctttca
    60961 tcaggaattc gcgggaacgc atgacgccga aacgcggacg cacttcgtcg cggaacttgg
    61021 tctggatctg atagaagttc agcggcagct gtttgtaaga gctaagctcg ttacgaatca
    61081 ggtcagtgat aacttcttca tgagttgggc cgagtacgaa cggacgctcg ccacggtcaa
    61141 caaaacgcag cagttccgga ccgtactgtt cccaacgacc actctcttgc cacaaatcgg
    61201 ctggctgaac caccggcatc gacacctcga ttgcaccggc gttgttcatc tcttcacgca
    61261 cgatgttttc gacttttttc agaacgcgca cgccggtcgg cagccaggta tataacccgg
    61321 aggccagctt gcggatcatc ccggcgcgca gcatcagctg atggctgatc acctcggcgt
    61381 cggcaggtgt ctccttgaga gtggagagca ggtattggct agtacgcatg ttgttacggt
    61441 tccagttgga aggtagaaca ggctcaaggc gagcctggga caaaaaaagt gatttagttt
    61501 accagtgcaa aagaaatgtc aaaagagaag ggcgtgaatt taacgcggtt ccagcgcaaa
    61561 gacttcaaaa cctgcgtcgg tgacgcgcca gcgaacgtta aaatcatgta gccagacggc
    61621 ataggttttg cccgtttcct cacctttatg ataggccggg cgcgggtcct gcgccagtac
    61681 ttcgcggata aacagcgtta actgcggata acgcttctcc agcgtcaaaa gctgcttttc
    61741 gacctctgcg gtaaaactca ccgccatctc tgcagctggc gcactttgcg catagctggc
    61801 actggcatcg ggaagcgatt cggcaaaggg gagatacggt ttgatatcca ctaccggcgt
    61861 accatcgacc agatccagac tgccgagctt cagaatcacg ctgtctttat ggcaaacaac
    61921 ctctttcagc tctaccagcg acataccaat tgggttaggg cggaaagtag agcgtgttgc
    61981 gaaaaccccc attctggcgt taccgccgag gcgcggtgga cgcacagtcg gacgccagcc
    62041 gccttccatc gtttgatgaa agacgaaaag gatccataaa tggctgaacg cttccaggcc
    62101 gcgaacggcg tcggcctggt tgtagggagc aatgagatgc agttcgccgt tggcgctttt
    62161 taccagacct ggctggcgcg gaacggcgaa tttttcttta tagggcgagc gaataacgcc
    62221 tatttgctcg aactggaaac tgctcatttc gccgtaatgt taagcgcaga gccgatacat
    62281 acagcctgac gatagcagcc tggcgtaccg ctggtgactt cgcagctatg cagtaatacc
    62341 gcattggctt tcattttaga ggcgttgatt tgcatccgct tacgcgcggt tggaatgctc
    62401 ggcggagagt cctgattaga ggcctggcaa gagtcgccac tgacttcacc gagatcgcgg
    62461 aacggtttgc cgactaattc ttctgcattg gtataaattc ggaccggcgt ggcgcgcggc
    62521 gctttcggtt ttgcaggctc cgctttcggc tggggtgcag tgctttgaac gggttcgaca
    62581 ggggatctgc ttaacatgga acagccgctt agcatgagtg ctactaaaca gatcggtaaa
    62641 gcacgcatag tatttcctca atgtatgatc aaaacgtcaa tattgaatca ggagcttgta
    62701 aaaatgacaa gacgggcaag tgcccatcct gaatgatatt acaaattgtg gaaacagcct
    62761 aaaaattacc agcctttaac agcgccgccg ttaaacactt tgtttgctgc ttcgtaaact
    62821 tcatcagact gataagcctg gacgaatttc ttcacgttct cggcgtcttt gttatcttca
    62881 cgcgttacga tcaggtttac gtacggggag tctttatctt cgacaaagat accgtctttc
    62941 gctggagtca ggccaatctg gctggcatag gtggtattga taactgccag agcgatttgt
    63001 gcgtcgtcca gagatcgagg cagctgcggt gcttccagct caacaatttt cagatttttt
    63061 gggttctcaa caacatcaag tacggtcggc agcaggccaa cgccatcttt cagtttgatc
    63121 aagcccactt tttgcagcag cagcagtgaa cgaccaaggt tagttggatc gtttggcacg
    63181 gcaacctgcg aaccatcctg cagttcatcc agtgatttga ttttcttgga gtaaccagca
    63241 atcggataaa caaaagtgtt gcctactgcg accagtttgt agccacgatc tttcagttgc
    63301 tgatcaaggt acggtttatg ctggaaggcg ttggcgtcga tatcgccttt gctcaatgct
    63361 tcgtttggca gaacatagtc gttgaaggtt accagctcaa cgtccaggcc atatttgtct
    63421 ttcgcaactt tctgcgcgac ttctgcaacc tgctgttcgg caccaacaat cacgccgact
    63481 ttaatgtggt ttggatcttt ttcatcctga ccgcagccta ccagtgccag tgatccaatc
    63541 agggctccca ctgccgcaaa ggttttgaat ttgaacgcca taccttattc cttcttcaat
    63601 tatttatgtt gtgttgaacg ttacttgcga gtgacagccc ggacgatgcg gtcgcctgcg
    63661 aactgaatta aataaaccag aatgaccagc aataccagta ccgtattcat caccgtcgcg
    63721 ttatagccga tgtagccata ctgatagcca atctgaccta aaccaccggc accgacagca
    63781 ccacccatcg cggaataacc gactagggta atcagggtga tagttgccgc attcaccaga
    63841 cccggcaatg cttccggtaa cagcacttta cggacgatct gcatcggcgt ggcacccatt
    63901 gcacgggaag cttcaattaa cccggttggg atctccagca gagcgttctc gaccatacgg
    63961 gcaataaacg gtgctgcacc aacggttaac ggaacaatcg ctgcctgcaa accaatcgat
    64021 gtaccaacaa taacgcgggt aaacggaatc atccatacca gcaagataat gaacgggata
    64081 gaacggaaaa tgttcacaat cgcagaaacg gtacgataca gcttcgcgtt agcaataatt
    64141 tgccccggac gcgtgacata aagcagaacg ccaaccggca gaccaatcac aaagccaaaa
    64201 aaaccggata cgaaggtcat tgccagcgtt tcccatacgc cacgaaccag cagccacatc
    64261 atcggctcag acataaccca gtacctctac ttttacatgg tgttcctgca gccaggcaat
    64321 ggcggcttgc gtatcttgtt gtgtgccgtg catttcagtc agcatgatgc cgaacttaac
    64381 gccaccggcg tagtccatct gcgcgctaat aatgttgttg ttgacgttga aacgacgcgc
    64441 ggtttcagaa agcagtgggg catcgaccga ttgaccggta aactccagac gcagcatcgg
    64501 cacgcagtca gtaaatggct ccgcttgcag acgttcctgg taatcttccg ggatatccag
    64561 atgcagggtc gactgaataa acttctgcgc cagcggcgtt ttcggatgcg agaacacttc
    64621 acttaccgtg tcctgctcga tcagttcgcc attgctgatg accgccacgc aatcacaaat
    64681 acgcttcaca acgtccattt cgtgggtgat caacagaatc gtcaaaccca gacggcggtt
    64741 aatgtctttc agcagttcga gaatagaacg tgtcgttgcc gggtccagcg cgctggtggc
    64801 ttcatcacac agcaatactt tgggattgct ggctaatgca cgggcaatcg ccacacgttg
    64861 tttctgccca ccggaaagat tcgacgggta gctatcatgc ttatcgccaa gaccaaccaa
    64921 tgacagcaat tccgtcacgc gacgtttgat ctcgtctttc ggtgtgttgt ccagctccag
    64981 cggcagagcc acgttgccaa aaacagtacg cgaagagagc aggttaaaat gctggaaaat
    65041 cataccaatc tggcggcgag ctttggtcaa ctcggattct gacagcgtgg tcagttcctg
    65101 gccatcgacc agcacgctac cctcggttgg gcgctccagc aggtttacac aacgtataag
    65161 cgtactctta cccgcgcctg aggcaccgat aacgccataa atttgtccag ctggcacatg
    65221 caggctgacg ttgttcaacg cctggatggt gcgggtgccc tggtggaaca ctttggtgat
    65281 attcgaaagt tttatcattg attatttatt atcgtcatta agttagtcgt ggcatctcga
    65341 atgcctgaaa cgggcaacgc cgtcaatgaa atggatgtta aggcatccag acgtctaaat
    65401 caatcaggtt tatgcgaaga gcactttctt gcaggtcgaa acatgcgata ctagcgtcac
    65461 atgccttatt aaggagctat aaaaggtggc aaagagcgta cccgcaattt ttcttgaccg
    65521 tgatggcacc attaatgtcg atcacggcta tgtccatgag atcgacaact ttgaatttat
    65581 cgacggtgtt attgacgcca tgcgcgagct aaaaaaaatg ggctttgtgc tggtggtagt
    65641 aaccaaccag tctggcattg ctcgcggtaa atttaccgaa gcacagtttg aaacgctgac
    65701 cgagtggatg gactggtcgc tggcggaccg agatgtcgat ctggatggta tctattattg
    65761 cccgcatcat ccgcagggta gtgttgaaga gtttcgccag gtctgcgact gccgtaaacc
    65821 acatccgggg atgcttttgt cagcacgcga ttatttgcat attgatatgg ccgcttctta
    65881 tatggtgggc gataaattag aagatatgca ggcagcggct gcggcgaacg tgggaacaaa
    65941 agtgctggtg cgtacgggta aacctattac gcctgaagca gaaaacgcgg cggattgggt
    66001 gttaaatagc ctggcagacc tgccgcaagc gataaaaaag cagcaaaaac cggcataatg
    66061 attaaaagat gagcgattga aataaaaatg catttttcca cttgtcttcc tgagacgact
    66121 ccctataatg cgcctccatc gacacggcgg atgtgaatca cttcacacaa acagccggtt
    66181 cggttgaaga gaaaaatcct gaaattcagg gttgactctg aaagaggaaa gcgtaatata
    66241 cgccacctcg cgacagtgcg ctaaagtgcg tcgcaactgc tctttaacaa tttatcagac
    66301 aatctgtgtg ggcactcgaa gatacggatt cttaacgtcg caagacgaaa aatg
//
LOCUS       DAJTMC010000004        61589 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.004, whole genome shotgun sequence.
ACCESSION   DAJTMC010000004 DAJTMC010000000
VERSION     DAJTMC010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 61589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61589
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.004"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            <1..362
                     /locus_tag="OO130_000239"
     CDS             <1..362
                     /locus_tag="OO130_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HCR5363782.1"
                     /translation="LDDSALDTEFSIGGTELLLFKQMGKATVDGIQLRFTGSIQRDDT
                     GEVQAVELVVRGRHKEVDSGEWKTGESNTTKVTSTNSYAKLTINGEVLYEVDLINMVE
                     IVDGVDLMEAHRNALGL"
     gene            417..719
                     /locus_tag="OO130_000240"
     CDS             417..719
                     /locus_tag="OO130_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HCR5363783.1"
                     /translation="MSDKQTEKTIQLDTPIMRGKTEITEIVLRKPQSGALRGTRLQAI
                     MDMDVNAMMTVIPRISSPALTAQEIAEMDPADLTAMSVEVVTFLLKKSVLAGLPTA"
     gene            734..853
                     /locus_tag="OO130_000241"
     CDS             734..853
                     /locus_tag="OO130_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HCR5363784.1"
                     /translation="MADIATIFHWPPSVTDVMPLTEVLEWRDKAIQRSGASHE"
     gene            846..3923
                     /locus_tag="OO130_000242"
     CDS             846..3923
                     /locus_tag="OO130_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR5363785.1"
                     /translation="MSDNNLRLQVILNAVDKLTRPFRSAQASSRELAAAVKKSRDAIK
                     QLDQAGSSLDSFRKLQAENQKLGDRLNYARQRANLLSQELGAMGPPSQRQVVALGRQR
                     LAVQRLEERQKKLQQQTALVRAELYRAGISANDGASATARITRETMRYNRQLSEQEAR
                     LRRVGEQQRKMHAARGAYARRLEVRDRIAGAGATTTAAGLAMGAPVMAAVKSYTSMED
                     AMKGVAKQVNGLRDDNGNRTARFYEMQGAIKAASEQLPMENGAVDFAALVEGGARMNV
                     ANPDDSWEDQKRDLLAFASTAAKAATAFELPADELSESLGKIAQLYKIPTRNIEQLGD
                     ALNYLDDNAMSKGADIIDVMQRLGGVADRLDYRKAAALGSTFLTLGAAPEVAASAANA
                     MVRELSIATMQSKSFFEGMNLLKLNPEVIEKQMTKDAMGTIQRVLEKVNALPQDKRLS
                     AMTMLFGKEFGDDAAKLANNLPELQRQLKLTAGNDALGSMQKESDINKDSLSAQWLLV
                     KTGAQNTFSSLGETLRQPLMDILYTVKSVTGALRRWVEANPELTGTLMKVAAVVAAVT
                     VGLGTLAVALAAVLGPLAVIRLGFSVLGIKTLPSVTATVTRTSNALSWLAGAPLALLR
                     RGLASSGNAAGLLTAPLSSLRRTASLTGNVLKTVAGAPVALLRSGLSGLRAVAVMFMN
                     PLAVLRGGLAATGTVLRMLASGPLAMLRVALYAVSGLLGALLSLIGLVVTALAGVALV
                     VWKYWQPITAFLGGVVEGFKAAAGPVSAAFEPLKPVFQWIGDKVQALWGWFTDLLTPV
                     KSTSAELQSAAAMGRRFGEALAEGLNMVMHPLDSLKSGVSWLLEKLGIVSKEAAKAKL
                     PESVTRQQPATVNADGKVMMPSGGFPSWGYGFAGMYDSGGYIPRGQFGIVGENGPEIV
                     NGPANVTSRRNTAALAAVVAGMMGVAATPAELPPLHPLALPAKGGEAMVSRAATVPPV
                     QRIEAPMQIIIQTQPGQSAQDIAREVARQLDERERRLKAKARSNYSDQGGYDA"
     gene            3920..4405
                     /locus_tag="OO130_000243"
     CDS             3920..4405
                     /locus_tag="OO130_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5363786.1"
                     /translation="MMMVLGLYVFMLRTVPYQELQYQRSWRHAANSRVNRRPSTQFLG
                     PDNDMLTLSGVLMPEVTGGRLSLLALEQMAEQGKAWPLIEGSGTIYGMYVIEGLNQTK
                     TEFFRDGMPRRIEFTLSLKRVDESLSDMFGDLSAQLNNLQGTATSALSDISKTVGGLL
                     S"
     gene            4402..5502
                     /locus_tag="OO130_000244"
     CDS             4402..5502
                     /locus_tag="OO130_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HCR5363787.1"
                     /translation="MNFSSELLNKGNKTPAFSISIEGRDITTVLDNRLMGLTLTDNRG
                     FEADQLDLELDDADGKIVLPRRGAVITLALGWKGQPLFPKGAFTVDEIEHTGAPDRLT
                     IRARSADFRETLNTRREKSWHKTTVGEVVKEIAARHKLKMALGEDLSDKLVEHIDQTN
                     ESDGSFLMRLARQYGAIASVKNGNLLFIRQGQGKSASGKPLPVITITRKDGDSHRFTL
                     ADRGAYTGVIASWLHTREPAKKESTTVKRKRRTKKQKKEPEAKQGDYLVGTDENVLVL
                     NRTYANRSNAERAAKMQWERLQRGVASFSLQLAEGRADLYTEMPVKVSGFKQPLDDAE
                     WTITTLTHTVSPDNGFTTSLELEVRIDDFEME"
     gene            5593..5811
                     /locus_tag="OO130_000245"
     CDS             5593..5811
                     /locus_tag="OO130_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001504085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HCR5363788.1"
                     /translation="MMICPLCGSAAHTRSSFQVSSLTKERYNQCQNINCSHTFVTHET
                     FVRSIATPKESNPVQPHPMKSGQVALSL"
     gene            complement(6046..7731)
                     /locus_tag="OO130_000246"
     CDS             complement(6046..7731)
                     /locus_tag="OO130_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HCR5363789.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRI
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            8001..8378
                     /locus_tag="OO130_000247"
     CDS             8001..8378
                     /locus_tag="OO130_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HCR5363790.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(8408..8665)
                     /gene="grxA"
                     /locus_tag="OO130_000248"
     CDS             complement(8408..8665)
                     /gene="grxA"
                     /locus_tag="OO130_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HCR5363791.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            8825..9112
                     /locus_tag="OO130_000249"
     CDS             8825..9112
                     /locus_tag="OO130_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HCR5363792.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            9096..9818
                     /gene="nfsA"
                     /locus_tag="OO130_000250"
     CDS             9096..9818
                     /gene="nfsA"
                     /locus_tag="OO130_000250"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HCR5363793.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQCEAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            9879..10781
                     /gene="rimK"
                     /locus_tag="OO130_000251"
     CDS             9879..10781
                     /gene="rimK"
                     /locus_tag="OO130_000251"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HCR5363794.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERYATTEYCLKTGG"
     gene            10869..11345
                     /locus_tag="OO130_000252"
     CDS             10869..11345
                     /locus_tag="OO130_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HCR5363795.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            11696..12808
                     /gene="potF"
                     /locus_tag="OO130_000253"
     CDS             11696..12808
                     /gene="potF"
                     /locus_tag="OO130_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HCR5363796.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            12903..14036
                     /gene="potG"
                     /locus_tag="OO130_000254"
     CDS             12903..14036
                     /gene="potG"
                     /locus_tag="OO130_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HCR5363797.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIASRVHEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERHEDGLVLYSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            14046..14999
                     /gene="potH"
                     /locus_tag="OO130_000255"
     CDS             14046..14999
                     /gene="potH"
                     /locus_tag="OO130_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HCR5363798.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVAAAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            14996..15841
                     /gene="potI"
                     /locus_tag="OO130_000256"
     CDS             14996..15841
                     /gene="potI"
                     /locus_tag="OO130_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HCR5363799.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARHG"
     gene            15901..16389
                     /locus_tag="OO130_000257"
     CDS             15901..16389
                     /locus_tag="OO130_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HCR5363800.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            16430..17587
                     /gene="rlmC"
                     /locus_tag="OO130_000258"
     CDS             16430..17587
                     /gene="rlmC"
                     /locus_tag="OO130_000258"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HCR5363801.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSETKLAQLRKALPWLQE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGFRIERVQLFDMFPHTAHYEVLTLLVKQSEAAGAASDC"
     gene            complement(17731..18462)
                     /gene="artJ"
                     /locus_tag="OO130_000259"
     CDS             complement(17731..18462)
                     /gene="artJ"
                     /locus_tag="OO130_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein ArtJ"
                     /protein_id="HCR5363802.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(18753..19421)
                     /gene="artM"
                     /locus_tag="OO130_000260"
     CDS             complement(18753..19421)
                     /gene="artM"
                     /locus_tag="OO130_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HCR5363803.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(19421..20137)
                     /gene="artQ"
                     /locus_tag="OO130_000261"
     CDS             complement(19421..20137)
                     /gene="artQ"
                     /locus_tag="OO130_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HCR5363804.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESEKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(20144..20875)
                     /gene="artJ"
                     /locus_tag="OO130_000262"
     CDS             complement(20144..20875)
                     /gene="artJ"
                     /locus_tag="OO130_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5363805.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYNSYQNAK
                     LDLQNGRIDGVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(20893..21621)
                     /gene="artP"
                     /locus_tag="OO130_000263"
     CDS             complement(20893..21621)
                     /gene="artP"
                     /locus_tag="OO130_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HCR5363806.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(21839..22354)
                     /locus_tag="OO130_000264"
     CDS             complement(21839..22354)
                     /locus_tag="OO130_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5363807.1"
                     /translation="MRYSKLTMLIPCALLLSACTTVTPAYKDNGTRSGPCVEGGPGNV
                     AQQFYDYRILHRSNDITALRPYLSDKLATLLSDASRDNNHRELLTNDPFSSRTTLPES
                     ARVASASTIPNRDARNIPLRVDLKQGDQSWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            22480..22803
                     /locus_tag="OO130_000265"
     CDS             22480..22803
                     /locus_tag="OO130_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HCR5363808.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            22800..23630
                     /gene="amiD"
                     /locus_tag="OO130_000266"
     CDS             22800..23630
                     /gene="amiD"
                     /locus_tag="OO130_000266"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HCR5363809.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(23627..24637)
                     /locus_tag="OO130_000267"
     CDS             complement(23627..24637)
                     /locus_tag="OO130_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCR5363810.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMFSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLCSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     FRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGKL
                     PR"
     gene            complement(24736..26166)
                     /locus_tag="OO130_000268"
     CDS             complement(24736..26166)
                     /locus_tag="OO130_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HCR5363811.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPASEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWQVVNQIGGKERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVD
                     SWKVIVVEPEKQLTLLFGMKAPGLGRLCFSLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            complement(26177..27178)
                     /gene="ltaE"
                     /locus_tag="OO130_000269"
     CDS             complement(26177..27178)
                     /gene="ltaE"
                     /locus_tag="OO130_000269"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HCR5363812.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKVRNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(27215..28933)
                     /gene="poxB"
                     /locus_tag="OO130_000270"
     CDS             complement(27215..28933)
                     /gene="poxB"
                     /locus_tag="OO130_000270"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HCR5363813.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKADRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIVVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(29066..30034)
                     /gene="hcr"
                     /locus_tag="OO130_000271"
     CDS             complement(29066..30034)
                     /gene="hcr"
                     /locus_tag="OO130_000271"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HCR5363814.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVHTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAKAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(30046..31698)
                     /gene="hcp"
                     /locus_tag="OO130_000272"
                     /gene_synonym="priS"
     CDS             complement(30046..31698)
                     /gene="hcp"
                     /locus_tag="OO130_000272"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HCR5363815.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVSAYDDRIWTRS
                     IVGWPGVRHLDGDDFSAVITQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATIVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLADKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            complement(31842..32741)
                     /gene="lysO"
                     /locus_tag="OO130_000273"
     CDS             complement(31842..32741)
                     /gene="lysO"
                     /locus_tag="OO130_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HCR5363816.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            complement(33235..33930)
                     /gene="aqpZ"
                     /locus_tag="OO130_000274"
     CDS             complement(33235..33930)
                     /gene="aqpZ"
                     /locus_tag="OO130_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HCR5363817.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATAKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRD"
     gene            34356..36014
                     /locus_tag="OO130_000275"
     CDS             34356..36014
                     /locus_tag="OO130_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HCR5363818.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAIEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(36011..36967)
                     /locus_tag="OO130_000276"
     CDS             complement(36011..36967)
                     /locus_tag="OO130_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF535 domain-containing protein"
                     /protein_id="HCR5363819.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLKLTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            37118..38233
                     /gene="macA"
                     /locus_tag="OO130_000277"
     CDS             37118..38233
                     /gene="macA"
                     /locus_tag="OO130_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HCR5363820.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQDLDTAATEMAVKQAQI
                     GTIDAEIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            38230..40176
                     /gene="macB"
                     /locus_tag="OO130_000278"
     CDS             38230..40176
                     /gene="macB"
                     /locus_tag="OO130_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HCR5363821.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGITLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHMVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALVRE"
     gene            complement(40249..40473)
                     /gene="cspD"
                     /locus_tag="OO130_000279"
     CDS             complement(40249..40473)
                     /gene="cspD"
                     /locus_tag="OO130_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HCR5363822.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            40796..41116
                     /gene="clpS"
                     /locus_tag="OO130_000280"
     CDS             40796..41116
                     /gene="clpS"
                     /locus_tag="OO130_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HCR5363823.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            41147..43423
                     /gene="clpA"
                     /locus_tag="OO130_000281"
     CDS             41147..43423
                     /gene="clpA"
                     /locus_tag="OO130_000281"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HCR5363824.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(43767..43854)
                     /locus_tag="OO130_000282"
     tRNA            complement(43767..43854)
                     /locus_tag="OO130_000282"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(43818..43820),aa:Ser,seq:gga)
     gene            complement(44108..44326)
                     /gene="infA"
                     /locus_tag="OO130_000283"
     CDS             complement(44108..44326)
                     /gene="infA"
                     /locus_tag="OO130_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HCR5363825.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(44611..45315)
                     /gene="aat"
                     /locus_tag="OO130_000284"
     CDS             complement(44611..45315)
                     /gene="aat"
                     /locus_tag="OO130_000284"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HCR5363826.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(45357..47078)
                     /gene="cydC"
                     /locus_tag="OO130_000285"
     CDS             complement(45357..47078)
                     /gene="cydC"
                     /locus_tag="OO130_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HCR5363827.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMMVTIGLSFLDF
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRITDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALAESL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(47079..48845)
                     /gene="cydD"
                     /locus_tag="OO130_000286"
     CDS             complement(47079..48845)
                     /gene="cydD"
                     /locus_tag="OO130_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HCR5363828.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQCGEVELASTDPVTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEHELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(48968..49933)
                     /gene="trxB"
                     /locus_tag="OO130_000287"
     CDS             complement(48968..49933)
                     /gene="trxB"
                     /locus_tag="OO130_000287"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HCR5363829.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            50478..50972
                     /gene="lrp"
                     /locus_tag="OO130_000288"
     CDS             50478..50972
                     /gene="lrp"
                     /locus_tag="OO130_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HCR5363830.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            51107..55057
                     /gene="ftsK"
                     /locus_tag="OO130_000289"
     CDS             51107..55057
                     /gene="ftsK"
                     /locus_tag="OO130_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HCR5363831.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDEE
                     EIIYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVTSVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQPQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPVQQPYYAPAAEQPVQQPYYATAPEQPAQQPYYAPA
                     PEQPVAGNAWQAEEQQSTFAPQSTYQTEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPV
                     VEETKPARPPLYYFEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSAAAVP
                     PVEAAAAVSPLASGVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKR
                     IRVPTRRELASYGIKLPSQRAAEEKAREARRNQYDSGDQYNDDEIDAMQQDELARQFA
                     QTQQQRYGEQYQHDVPVNAEDADAAAEAELARQFAQTQQQRYSGEQPAGANPFSLDDF
                     EFSPMKALLDDGPHEPLFTPIVEPVQQPQQLVAPQQQYQQPQQQVAPQPQYQQPQQPV
                     APQQQDTLLHPLLMRNGDSRPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVE
                     ARLADFRIKADVVNYSPGPVITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEV
                     IPGKPYVGLELPNKKRQTVYLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMP
                     HLLVAGTTGSGKSVGVNAMILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVV
                     TDMKDAANALRWCVNEMERRYKLMSALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGD
                     SMDAQHPVLKKEPYIVVLVDEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRP
                     SVDVITGLIKANIPTRIAFTVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRV
                     HGAFVRDQEVHAVVQDWKARGRPQYVDGITSDSESEGGAGGFDGAEELDPLFDQAVQF
                     VTEKRKASISGVQRQFRIGYNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            55212..55823
                     /gene="lolA"
                     /locus_tag="OO130_000290"
     CDS             55212..55823
                     /gene="lolA"
                     /locus_tag="OO130_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HCR5363832.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            55834..57177
                     /gene="rarA"
                     /locus_tag="OO130_000291"
                     /gene_synonym="mgsA"
     CDS             55834..57177
                     /gene="rarA"
                     /locus_tag="OO130_000291"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HCR5363833.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVDDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPREIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            57268..58560
                     /gene="serS"
                     /locus_tag="OO130_000292"
     CDS             57268..58560
                     /gene="serS"
                     /locus_tag="OO130_000292"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HCR5363834.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            58799..61243
                     /gene="dmsA"
                     /locus_tag="OO130_000293"
                     /pseudo
     CDS             58799..61243
                     /gene="dmsA"
                     /locus_tag="OO130_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
     gene            61254..>61589
                     /locus_tag="OO130_000294"
     CDS             61254..>61589
                     /locus_tag="OO130_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HCR5363835.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYG"
BASE COUNT        15094 a        15457 c        16619 g        14419 t
ORIGIN      
        1 gactggatga cagtgcactg gatacagaat tttccattgg tggtactgaa ctgctgctgt
       61 ttaagcagat gggcaaagcc acggtggatg gcatccagct gcgctttacc ggctctattc
      121 agcgtgatga taccggggaa gtgcaggccg tggagcttgt ggtgcgtgga cgtcacaaag
      181 aagtagattc cggcgagtgg aagacgggcg aaagcaacac taccaaagtg accagtacca
      241 acagctacgc gaagctgacc atcaatggtg aggtgctcta tgaagtggac ctgatcaaca
      301 tggtggaaat tgtggacggt gtggacctga tggaagcgca ccgcaacgcc ctcggcctct
      361 gatgtatctg aacggcgcgg aatgccgcgc cagaacctaa tttacaggac aacaaaatga
      421 gcgataagca gactgaaaag accattcaac tggatactcc catcatgcgc ggtaaaacag
      481 aaattaccga aattgtgttg cgtaaaccgc agtccggtgc gctgcgcggc acacgcctgc
      541 aggccattat ggatatggat gtgaacgcga tgatgaccgt gatcccccgc atctccagtc
      601 cggcactgac tgcacaggaa attgcagaga tggacccggc agatctcact gccatgtcgg
      661 ttgaggttgt cacttttttg ttgaagaagt cggtgcttgc cggtttaccg acagcctgac
      721 ggttgacgat ctggtggctg atatcgccac catctttcac tggccgccat ccgttactga
      781 cgttatgccg ctgaccgaag tgctggaatg gcgggataaa gcgattcaga gaagcggggc
      841 cagccatgag tgacaataac ctgcgtctgc aggtgattct taatgcggtt gacaaactca
      901 cccgcccatt tcgatccgcg caggccagtt caagagaact ggctgctgct gtcaaaaaat
      961 cccgcgatgc aataaagcag cttgatcagg ccgggagcag tctggacagc ttccgaaagc
     1021 tgcaggcaga aaatcagaaa ttaggcgaca ggctgaacta tgcccgccag cgtgcaaatt
     1081 tgctcagtca ggaactggga gcgatggggc cgccttcgca acgtcaggtt gttgctctgg
     1141 gccgtcaacg gctggctgtt cagcgcctag aagaacgcca gaaaaagctg cagcagcaga
     1201 cggcgcttgt gcgtgcagaa ctttatcgtg ctggtatttc agctaatgat ggcgccagtg
     1261 cgacggcccg cattacccgt gaaacgatgc gttataacag gcagctttct gagcaggaag
     1321 cgaggttacg acgtgtcggg gagcaacagc gaaaaatgca cgccgcccgg ggggcttacg
     1381 ccaggcgtct tgaggtaagg gatcgtattg caggtgccgg agccaccacc acggctgcag
     1441 ggctggcaat gggcgcacca gtgatggcgg cagtaaaaag ctataccagc atggaagatg
     1501 ccatgaaagg tgtggcaaag caggtcaatg gtctgcgtga cgataatggc aaccgcactg
     1561 cgcgttttta cgaaatgcag ggtgccatca aggctgccag cgaacagttg ccgatggaaa
     1621 acggtgctgt ggacttcgcc gcactggttg aaggtggtgc gcgcatgaac gtcgcaaacc
     1681 ctgacgacag ctgggaagac cagaaacgtg acctgctggc cttcgccagc acggcggcaa
     1741 aggcggcaac agcctttgaa ctgccagcgg atgaactgtc agaaagtctg gggaaaatcg
     1801 cccagctcta caaaatccct acccgcaata ttgagcagct cggtgatgcg ctgaactatc
     1861 tggatgataa cgccatgtcg aaaggggcgg acatcattga tgtcatgcaa cgcctgggcg
     1921 gtgtggctga ccgtctggat tatcgtaaag cggcggcgtt gggttccacc ttcctgacac
     1981 tcggcgctgc gcctgaggtt gcagccagtg cagcaaacgc gatggtgcgt gaattgtcca
     2041 ttgccaccat gcaaagcaag agtttctttg aagggatgaa tctgctgaaa ctcaatccag
     2101 aagtgattga aaagcagatg acgaaggatg cgatgggaac tatccagcgg gtgctggaga
     2161 aggtgaatgc gctgccacag gataagcgcc tgtctgccat gaccatgttg tttggtaaag
     2221 agtttggcga tgacgcagcg aaactggcaa acaacctgcc ggaacttcag cgccagttaa
     2281 aactgactgc gggcaatgat gcgctcggtt ccatgcagaa agaatccgac atcaacaagg
     2341 attcactttc tgctcagtgg ttgctggtca aaaccggagc acagaacacc ttcagcagcc
     2401 tgggggaaac gctgcgccag ccgctgatgg atattctgta cacggtgaaa agcgtcacgg
     2461 gggcgttgcg tcgctgggtg gaagctaacc cggaactgac gggcactctg atgaaagtag
     2521 ccgctgtggt agctgccgtt accgtgggcc tcggcacctt agcggtggcg ctggctgcag
     2581 tgctggggcc gctggcagtc atccgtctgg gattctctgt gctgggtatc aaaacgttac
     2641 cttccgttac ggcaacagta acccgaacca gcaacgcgtt gtcctggctg gctggcgcac
     2701 cactggcact gctgcgacgc gggcttgctt catcgggcaa cgctgcaggt ttacttactg
     2761 cgccgttgtc gtctttgcgc cgcacggcat cactgacggg aaatgtcctg aaaactgtag
     2821 caggtgcgcc ggttgcactt ttgcggtctg gattatccgg tttacgtgcg gttgctgtga
     2881 tgtttatgaa tccactggcg gtactacgcg gcggactggc tgccacaggc acggtgctgc
     2941 gtatgctcgc atccggtcca ctggcgatgc tgcgcgttgc cctgtatgcc gtctctggtc
     3001 tgttaggtgc tctgctcagt ctgataggtc ttgtggttac tgcactggcg ggtgtggcgc
     3061 tggttgtctg gaaatactgg cagcccatca ccgcatttct cggtggcgtg gtggaaggat
     3121 tcaaagcggc ggcaggtcct gtcagtgccg cgttcgaacc gcttaagcct gtgttccagt
     3181 ggattggcga caaagtacag gcgctgtggg gctggtttac tgatctgctg acgcccgtta
     3241 agtcgacctc tgccgaactg cagagtgcag cggcaatggg gcgacgattc ggggaggctc
     3301 tggcggaagg gctgaatatg gtcatgcatc cgctggactc cctgaaatcc ggcgtttcct
     3361 ggttgctgga gaaacttggc attgtcagta aagaggccgc aaaggcgaaa ctgccggaaa
     3421 gcgtgacgcg tcagcaacct gcgacggtga atgcagacgg taaagtgatg atgccatcgg
     3481 gtggttttcc gtcatgggga tatggctttg cggggatgta tgacagcggc gggtatatcc
     3541 cgcgcgggca gtttggcatc gtcggtgaaa acgggccgga aattgttaac ggcccggcaa
     3601 atgtgaccag ccggagaaat acagctgcac tggctgccgt tgttgccgga atgatgggtg
     3661 ttgctgctac gccagcagag cttccaccgt tgcacccttt ggcacttccc gcgaaaggcg
     3721 gtgaagcgat ggtgagtcgt gcagccactg tgccgcccgt tcaacggatt gaggcaccga
     3781 tgcagatcat cattcaaacg cagccaggac aaagtgcgca ggatattgcg cgggaggtgg
     3841 cccgccagct tgatgaacgt gaacgcaggc tgaaggcaaa agccaggagt aactacagcg
     3901 atcagggggg atacgacgca tgatgatggt gctgggattg tacgtgttta tgctgcgcac
     3961 cgtgccgtat caggagctgc agtatcaacg cagctggcga catgcggcaa acagccgggt
     4021 aaaccgacgc ccgtcaacgc agtttctggg accggacaac gacatgctga cgctttctgg
     4081 tgttcttatg ccggaggtaa cgggcggcag gctgtcgttg ctggctctgg agcagatggc
     4141 agaacaggga aaagcatggc ccctgattga aggcagcggc acgatttacg gcatgtatgt
     4201 gattgaggga ctgaatcaga ctaaaacgga gtttttccgc gacggtatgc cgcgccggat
     4261 tgagttcacc ctgtcgctaa aacgcgtgga tgaatccctg tccgatatgt tcggtgatct
     4321 cagtgcgcaa ctgaataatc tgcagggaac ggcaacatct gccttaagcg atatcagtaa
     4381 aacggtggga gggctgctgt cgtgaatttc agctctgaac tgcttaacaa aggcaacaaa
     4441 actccggcat tcagcatcag tattgaaggc agggatatca ccactgtgct ggacaaccgc
     4501 ctgatggggc tgacgctgac ggataaccgg ggctttgaag cggaccagct tgatctggag
     4561 ctggacgacg ccgatggaaa aatcgtgctg ccgcgccgtg gggctgtcat tacgctggcg
     4621 ctgggctgga agggacagcc gcttttcccg aaaggggcat tcacggtgga cgagattgaa
     4681 cacactggcg caccggatcg cctgactatc cgggcgcgaa gtgctgattt tcgtgaaacg
     4741 ctgaataccc gccgtgaaaa gtcgtggcac aagaccaccg ttggggaagt ggtgaaggaa
     4801 atagctgcgc ggcacaaact gaagatggca ttgggtgaag acttgtcgga taaactcgtg
     4861 gagcatatag accagactaa tgagagtgac ggcagttttt tgatgcggct ggcgcgccag
     4921 tacggtgcta ttgcgtcggt gaaaaatggc aatctgttat tcatccggca ggggcagggt
     4981 aaaagcgcca gcggtaaacc actaccggtg atcactatca cacgcaagga cggcgacagt
     5041 caccgcttta ccctggcaga tcgcggagct tacacgggcg tcattgccag ctggttgcat
     5101 acccgcgaac ccgcgaagaa agaaagcacc acggtgaagc gtaagcgcag gactaagaag
     5161 cagaagaaag agccggaagc gaagcagggc gattacctgg tgggtacgga tgaaaacgtg
     5221 ctggtactta atcgcactta tgccaaccgg agcaacgccg aacgggcagc gaaaatgcag
     5281 tgggaacgcc tgcaacgcgg cgttgcatca ttctcgctac aactggcgga agggcgggca
     5341 gatctctaca cagaaatgcc tgtgaaggtc agtggcttta aacagccgct agatgatgcg
     5401 gaatggacca ttacgactct gacgcatact gtcagcccgg ataacggttt tacgaccagt
     5461 ctggagcttg aagtgaggat tgatgatttc gaaatggaat gattcttcgc aatggagaac
     5521 ttttaagttt gcaaaatgga ataatgcggt atcattattg tgaatttagc aaaaatgggg
     5581 agaactcgaa aaatgatgat ttgcccactg tgtggaagtg ccgcccatac tcgcagcagt
     5641 tttcaggtat cttcattgac caaagagcgt tacaaccagt gccagaacat taactgcagc
     5701 catacttttg ttacccatga aacttttgtt cgttcgattg caacgccaaa agagtcaaat
     5761 ccggttcagc cgcatccaat gaaatcagga caggtggcgc tctctctttg acgctgccgc
     5821 cattttgtcg ccatcgttaa aaaacagtgc ttctaacatc atgattttaa aaggcataaa
     5881 tttcagacaa caaaaaccca tcaaccttga accgaaatgg cggggttgat gggctccaca
     5941 aaatggggac atcaaagaaa agcagtggca ctaattaaga ctgatgccct gcggaaaagt
     6001 tctgcggttg tgcaaaaaaa tttcattttc agggcaactt cagttttatc ctaatcctgg
     6061 ccataccatg acgatgattg tccctgccag cgtcagcagg acgttggcga ttgcgtaggt
     6121 gcccgcatag cccagcgcag ggatgttact gcgagctgta tcactgatga tctccattgc
     6181 cggcgcgcag gtgcgtgcgc ccatcattgc gccgaacaac agtgcgcggt tcattcgcaa
     6241 tacataagca ccgaacaaga aacagataac cacgggcaca agactgacaa ttaatccggc
     6301 aatcaacatc tgaccgccaa tcgcgcccag gccgttatta ataccgctac cggcgctcag
     6361 accaacgcct gccataaaca ccatcaagcc gaactctttc accatgctta atgcaccttg
     6421 cggaatgtaa ccgaaggtcg ggtggttagc acgcataaag cccagcataa ttccggcgaa
     6481 taacaacccg gcagcgttcc ccatgccgaa actgaatgtg ctgaactgga aggtgatcat
     6541 cccgatcatc agcccaataa caaagaaggc gcaaaatgcc agcaggtcag tgacctggct
     6601 gtgaatcgag ataaagccga tgcgatcggc gatggttttt acgcgacggg catcaccgct
     6661 gacttgtaaa acgtcacctt tgttaagcac gacgttgtca tctatcggca tctcaatctg
     6721 gctacgaatg atgcggttaa ggaagcaacc gtgatcggtc aacttcagtt gtgcgagacg
     6781 tttacctaca gcgttatggt ttttaacgac cacttcttca gtgacgatac gcatgtcgag
     6841 aaggtcacga tcgaaaactt ctttaccgtt acggaagctg ggatcgagtc gggcatgggc
     6901 gtcgggatag cctaccaacg ctatttcatc gcccatttgt agcacggcat caccgtctgg
     6961 atttgccaga atcccgttac gtcgaatacg ttcaatgtag cagccggttt gtcgataaat
     7021 acccagttca cgcagatttt tgccgtcggt ccaggccacc agctccgggc cgacgcgata
     7081 ggcgcggatc accggtaaat aaaccttacg gttggcatca gtgtccaggc cacgttcgcg
     7141 ggcgatttgc tgggcgctgg tctgtaagtc ctgatgctgc aatttcggca agtaacgcgc
     7201 accaacaatc aaactcacca gaccgattaa ataggttaag gcatacccga ggctcagatt
     7261 atccagtgcc agtgagagct gcctgctttc catgccggaa tgacgcagtg tatcgccagc
     7321 accgaccaga accggtgtcg acgtcataga gcctgctaac ataccggccg tcaggccaat
     7381 atcccagcca aacagcttac ctaaccctaa ggcgatcacc agcgcactgc caaccatcac
     7441 cagtgctaac attaggtaat ttttcccatc gcgaaaaaaa atggaaaaaa agttcggtcc
     7501 ggcttcgacc ccgacgcaga aaataaacag cataaagcca agattaagcg catcggtgtt
     7561 aatgctgaaa tgttgttggc ctaataacag cgatacgact aaaacgccaa tggaattacc
     7621 cagttggatc gaaccaagtc gtaactttcc gagacatagc ccaagcgcga ggaccacaaa
     7681 taataacaga atgtaattcc catttaacaa ttcggcgacg tttatattca cggaggctaa
     7741 cttcttgttt actagtaagc tgttgaaaga aatggtaatt tacgataatg ttttttacca
     7801 gaattcaggg cgcagattca ttcagcgcac ctaaacgata gtaaagtaac aatatatttt
     7861 actagtgtaa tcacattagg tatcaacggc tatatgaatt gcgttggcct atattagcat
     7921 ggaatgcgaa gcggctttat cttactgaac gccacactgg cgaaaaatgt gttcgataga
     7981 cgcagtgtca ggaggaacga gtgaaacata aacaacgttg ggcgggggca atctgctgtt
     8041 ttgtcctctt cattgtggtg tgcctttttc tggcgacgca catgaaaggc gcttttcggg
     8101 ctgccgggca tcctgaaatc ggcttgctgt ttttcattct tcctggagca gttgccagtt
     8161 tcttttcaca gcgtagagaa gtcctgaaac ctctatttgg cgcaatgctg gcggcaccct
     8221 gttcaatgct cattatgcgg ctgttttttt caccgacgcg ctcattctgg caagagctgg
     8281 catggttact aagcgcggtg ttctggtgtg cgctgggggc actgtgtttc ttatttatca
     8341 gtagtttgtt taaaccacag cacagaaaaa atcagtaaag ccctcaacgc gagggcttgt
     8401 cagacgatca ggcgtccaga ttttctttca cccatgcagc aaaatcggta tagccgccga
     8461 tatgttgctg atcgacaaaa atctgcggca cggtttctac gggtttacct gccttttgtt
     8521 gtagatcttc tttagtgatc ccttccgcac gaatatctac atactgatac tgaaaatcat
     8581 cgcgttcatt gctcaatttc tcagccagat cttttgcacg cacacagtaa gggcaacccg
     8641 aacgaccaaa aataacggtt tgcattattt ctctcctcat agatttatgc ctgtaatgat
     8701 cacgctaaaa tgtattcgct gaaagtaggt ttaacctgtt gcattaattg ctaaaagcta
     8761 taactgttaa acacaataca gtgaaaagtt ttagactgaa ggctcacttt gcagagggaa
     8821 gcgtatgcgc gcgatcggta aattgcctaa aggcgtgttg atactggaat ttatcggaat
     8881 gatgctactg gcggtggcgc tgctgtcggt aagcgactcc ctgtcgctgc ctgagccatt
     8941 ttctcggcca gaagtgcaga ttctgatgat ttttctcggt gttttgctca tgcttcccgc
     9001 tgcggtggtg gttattcttc aggtggcaaa acgtcttgcc ccacagctga tgaaccgtcc
     9061 accgcaatat tcacgttcag aaagagaaaa agataatgac gccaaccatt gaacttattt
     9121 gtggccatcg ctccattcgc catttcactg atgaacccat ttccgaagcg cagtgtgagg
     9181 cgattattaa cagcgcccgt gcgacgtcca gttccagttt tttgcagtgt agtagcatta
     9241 ttcgcattac cgacaaagca ttacgtgaag aactggtgac gctgaccggc gggcaaaaac
     9301 acgtagcgca agcggcggag ttctgggtgt tctgtgccga ctttaaccgc catttacaga
     9361 tctgtccgga tgctcagctc ggcctggcgg aacaactgtt gctcggtgtc gttgatacgg
     9421 caatgatggc gcagaatgca ttaaccgcag cggaatcgct gggattgggc ggggtatata
     9481 tcggcggcct gcgcaataat attgaagcgg tgacgaaact gctgaaatta ccgcagcatg
     9541 ttctgccgct gtttgggctg tgccttggct ggcctgcgga taatccggat ctcaagccgc
     9601 gtttaccggc ctccattttg gtgcatgaaa acagctatca accgctggat aaagacgcac
     9661 tggcgcagta tgacgagcaa ctggcggaat attacctcac ccgtggcagc aataatcgcc
     9721 gggatacctg gagcgatcat atccgccgaa caatcattaa agaaagccgc ccatttattc
     9781 tggattattt gcacaaacag ggttgggcga cgcgctaaaa ccgccacgtc gatgtatgat
     9841 acgcgggctt ttgaccaggt ctgacagaga ggtgcagggt gaaaattgcc atattgtccc
     9901 gggatggaac gctctattcg tgtaagcggc tgcgtgaagc cgctatacag cgcggtcacc
     9961 tggttgaaat tcttgatccg ctttcttgct acatgaacat aaatcctgcg gcgtcttcta
    10021 ttcactacaa aggccgcaag ttaccccatt ttgacgcagt gatcccgcgt attggcaccg
    10081 ccattacctt ttatgggacg gcggcactgc gccagttcga gatgctgggg agctatccgc
    10141 tcaatgagtc ggtcgccatt gcccgggcgc gtgacaaatt gcgttccatg caactgctgg
    10201 cgcgtcaggg catcgacctg cctgtcacgg gcattgcgca ttcgccggat gataccagcg
    10261 atttaatcga catggtcggt ggtgcgccgc tggtggtcaa gttggttgaa ggcacgcagg
    10321 gaattggcgt cgtgctggcg gagacgcgtc aggcggcgga aagcgtgatt gacgctttcc
    10381 gcggtctgaa cgcgcatatt ctggtgcagg aatatatcaa agaggcgcaa gggtgcgata
    10441 tccgctgtct ggttgttggc gatgaagtgg tcgctgcgat tgaacggcgg gcgaaagagg
    10501 gcgattttcg ttccaatttg catcgtggcg gcgcggcaag cgtcgccagt atcacaccac
    10561 aggagcgtga aatcgcgata aaagccgcgc gaacgatggc gctggacgtt gctggtgtgg
    10621 atattctgcg tgctaatcgc gggccgttgg tgatggaagt gaatgcgtcg ccggggctgg
    10681 aaggaataga aaaaaccact ggtatcgaca tcgcgggtaa aatgatccgc tggatcgaac
    10741 gctacgctac gacagaatat tgcctgaaaa cgggtggtta gtcgcaatca cattactgat
    10801 catggttttg cctgcgcttt ttgcgtaagc tgtgccggtc tttttatcga aagaggttgt
    10861 acaaaattat gacatcgctg gtcgttcctg gtctggatac gctgcgtcaa tggctcgatg
    10921 acctggggat gagttttttt gaatgtgata actgtcaggc tctgcatctg ccccatatgc
    10981 agaatttcga cggtgtcttt gatgccaaaa tcgatctgat cgataacacg atcctgtttt
    11041 ctgccatggc ggaagtccga ccttcagccg tattgccgct ggcggcggat ttatctgcca
    11101 tcaatgccag ttcgctgacc gtgaaagcat ttcttgatat gcaggatgat aatctgccaa
    11161 agctggtggt ttgccagtct ttatccgtta tgcagggcgt aacctacgag cagtttgcat
    11221 ggttcgtgcg tcagagcgaa gagcagattt cgatggtcat tcttgaagct aatgcccatc
    11281 aactgctgtt accgactgac gatgaagggc aaaacaacgt taccgaaaac tatttcctcc
    11341 actgataact cctttcgagc acgcagtcgc tggtgcagtg actgcgcact gcaaaattat
    11401 ctgctgtttt taaccttttc ttaaagatta tttcacttct cttgtgccga tttggcttta
    11461 tcacatagag caaatacgca taacaatttg ttaaataccg ttttttaatc cgagctatag
    11521 tctcaaaccc tggctaaagt tattcttgcg atgcttttat atagcgagca gtgctggccg
    11581 ggagagagtt atcttttctt acaccgcgcc gataaaaaat atgcacgttt attgcatatc
    11641 ttttagtatg acaacttttg ttcgtttgtt aacgaacttt cagaaggaaa gagatatgac
    11701 cgccttaaat aaaaaatggc tttcgggtct ggttgcgggt gctctgatgg ccgtctctgt
    11761 cggcacgctc gcggctgaac aaaaaacact ccacatttat aactggtctg attatatcgc
    11821 ccctgacacg gtggccaatt ttgaaaaaga aaccggtatt aaagtcgtct acgatgtttt
    11881 cgactctaac gaagtactgg aaggcaaatt aatggcgggt agcaccggct ttgacttggt
    11941 ggttccatct gccagctttc ttgaacgcca gttgactgcg ggagttttcc agccgctgga
    12001 caaaagcaaa ttgccggagt ggaagaatct cgacccggag ctgctgaagc tggtcgccaa
    12061 acacgatccc gacaataaat ttgctatgcc ctatatgtgg gcgacgaccg ggattggcta
    12121 taacgttgat aaagttaaag ctgtgctggg cgaaaacgcg cccgtcgata gctgggactt
    12181 gatcctcaaa cctgaaaatc tggaaaaact gaaaagctgc ggtgtctctt tcctggatgc
    12241 gccagaagaa gtttttgcta ccgtgttgaa ttatctcggc aaagatccca acagcactaa
    12301 agcggatgat tacaccggac cggcaaccga tctgctgtta aagctgcgcc cgaacattcg
    12361 ttatttccat tcatctcaat acattaacga cctggcaaac ggcgatattt gcgtcgctat
    12421 cggctgggca ggtgatgtct ggcaggcgtc aaaccgcgcg aaggaagcga agaatggagt
    12481 gaatgtctcg ttctcgattc caaaagaagg ggcgatggcg ttctttgatg tattcgccat
    12541 gcctgcggat gccaaaaaca aagacgaagc ctatcagttc ctgaattacc tgctgcgccc
    12601 ggatgtagta gcgcatattt ccgaccatgt gttctatgcc aacgccaata aagcagccac
    12661 gccgctggtg agtgcggaag tccgtgagaa cccgggtatt tatcctccgg cggacgttcg
    12721 tgcgaagcta ttcactctga aagtacagga tccgaaaatc gaccgtgtgc gcacccgcgc
    12781 gtggaccaaa gtgaagagcg gaaaataatc cgcagcctta gatgccgggc gggcgcacca
    12841 cacccgccgg caattcgcac catcatggtg cgcttgcaca cattcaatgc cggagagcag
    12901 ccgtgaatga cgctatccct cgcccgcagg cgaaaacccg taaggcgctg acgccgctat
    12961 tagaaatccg caacctcacc aaatcctacg atggtcagca tgcggtcgat gatgtcagcc
    13021 tgaccatcta caaaggtgaa atcttcgcgc tgctgggcgc ttccggctgt ggcaagtcca
    13081 cgctgctgcg tatgctggca ggttttgaac aaccttctgc cgggcagata atgctcgatg
    13141 gcgtcgattt gtcacaggtg ccgccttacc tgcgccccat caatatgatg tttcagtctt
    13201 acgcgctgtt tccgcatatg accgtggaac agaacatcgc ttttggcctg aaacaggaca
    13261 aactaccgaa agcggaaatt gccagtcggg tccatgagat gctcggcctg gtgcacatgc
    13321 aggagttcgc caaacgcaaa ccgcatcagc tttccggtgg tcagcgacag cgtgtggcgc
    13381 tggcccgaag ccttgccaag cgcccgaaac tattactgct cgacgagccg atgggcgcgc
    13441 tggataaaaa gctgcgtgac cggatgcagc ttgaagtggt ggatattctg gagcgtgtcg
    13501 gcgtgacctg tgtgatggtc actcacgatc aggaagaggc gatgacgatg gcgggacgta
    13561 tcgccattat gaatcgcggg aaatttgtcc agattggcga gccagaagag atctacgaac
    13621 atccaaccac ccgctacagt gctgaattta tcggttcggt taatgtcttt gaaggtgtac
    13681 tcaaagagcg tcatgaggat gggcttgtgc tttattcgcc tgggctggtg catccactga
    13741 aagtcgacgc ggatgcctcg gtggtcgata acgtgccggt acatgtggcg ctgcgcccgg
    13801 aaaaaatcat gctttgcgaa gagccgcccg ccaatggttg taacttcgcg gtgggggagg
    13861 tgatacacat tgcctatctc ggcgatcttt cggtgtatca cgttcgtctg aaaagtgggc
    13921 agatgattag cgcccagcta caaaacgccc atcgtcatcg taaagggtta ccgacctggg
    13981 gcgacgaagt gcgtttgtgc tgggaagtgg acagctgtgt ggtgctgacg gtttaaggag
    14041 caaagatgaa tacacttgaa cctgctgccc agtcgaaacc gccgggcgga tttaagctgt
    14101 ggttgtcgca gctgcaaatg aagcatgggc gcaaactggt cattgcgttg ccatatatct
    14161 ggttgatctt gctgtttctg ctgccatttc tgattgtctt taaaataagc ctggcggaga
    14221 tggcgcgcgc tattccacct tataccgaac tgatggagtg ggctgacggg caactgtcca
    14281 tcactcttaa tctcggtaat tttctgcaac tgaccgacga tccgctctat ttcgatgctt
    14341 atctccagtc gctacaggtg gctgcgattt cgacattttg ctgtttactg atcggctatc
    14401 cgctggcgtg ggcggtggcg cacagtaagc cttcgacccg taatatttta ttactactgg
    14461 tgatcctgcc gtcgtggacc tcgtttctga tccgcgttta tgcctggatg ggaatattaa
    14521 aaaacaacgg tgtgctgaat aattttctgc tgtggctggg ggttatcgat caaccgctga
    14581 ccattcttca taccaatctg gcagtttata ttggcattgt ttacgcctac gtgccgttta
    14641 tggtgctgcc gatttatacc gcgttgattc gtattgatta ttcgctagtg gaagcagcgc
    14701 tggatctcgg tgcacgaccg ctgaaaacgt tctttactgt gatcgtgccg ctgactaaag
    14761 gtgggattat tgccggatcg atgctggtgt ttatcccggc tgtgggcgag tttgtgatcc
    14821 cggaactgct cggtggcccg gacagcatca tgatcgggcg cgtgctatgg caagagtttt
    14881 tcaataaccg cgactggccg gtggccgcgg cggtagcgat catcatgttg ctgctgctaa
    14941 ttgtgccgat aatgtggttc cacaaacacc agcaaaaaag cgtgggagaa cacggatgaa
    15001 taatttaccg gtagttcgtt cgccctggcg gattgtgatt ttgctgctgg gctttacctt
    15061 tctctacgcg ccaatgctga tgctggttat ctattcgttt aacagctcga agctggtgac
    15121 ggtgtgggcc ggctggtcaa cgcgctggta tggtgagtta ttgcgcgatg acgcgatgat
    15181 gagtgcggtt ggtttaagcc tgacaattgc tgcctgtgcg gcaacggcgg cggcgatcct
    15241 cgggactatt gcggcggtgg tgctggtgcg ctttggcagg tttcgcggat caaatggctt
    15301 tgcctttatg atcacagcgc cgctggtaat gccagatgtc atcacgggct tgtcgctgtt
    15361 gttattattc gtcgcgcttg ctcatgccat tggctggcct gcggaccgcg gtatgcttac
    15421 catctggctg gcgcatgtca ctttctgtac ggcttatgtg gcggtcgtta tttcgtcgcg
    15481 tctgcgggaa ctggatcgct cgatagaaga agcagcgatg gatctcggtg cgacgccgct
    15541 gaaagtgttt ttcgtcatta cgctaccgat gatcatgcct gcgatcattt ctggctggtt
    15601 actggctttt actttgtcgc ttgatgatct ggtgatcgcc agctttgttt ccgggccggg
    15661 agccaccacg ttaccgatgc tggtcttttc cagcgtgcgg atgggggtga atccggaaat
    15721 caacgccctt gcaacgttaa ttctcggtgc ggtcggaatt gtcggattta tcgcctggta
    15781 tctgatggct cgcgcagaaa aacagcggat acgcgatatc cagcgtgcaa gacatggctg
    15841 aagacactaa aatttgccaa cctggctaca taatgccgcg catgccgcgg cattgttttc
    15901 atggaagacg aaacgttggg attttttaag aaaacatctt catctcatgc tcgcctgaat
    15961 gtgcctgcgc tggtgcaggt agcggcgctc gccattatta tgatccgtgg cctcgacgtg
    16021 ctgatgattt tcaatacgct gggcgtgcgc ggtattggcg agttcattca tcgcagcgta
    16081 caaacctgga gcttaacgct ggtattttta agcagtctgg tgctggtttt tatcgagatc
    16141 tggtgtgcgt tttcactggt gaaagggcgt cgctgggcgc gctggctata tctgctgaca
    16201 caaatcaccg ccgcaagtta cttgtgggcg gcttcgctgg ggtatggtta tccggagctg
    16261 ttcagcattc ccggtgaatc aaaacgtgaa atcttccata gcctgatgct gcagaagctg
    16321 ccggatatgc tcatcctgat gctgctgttc gttccctcga ccagtcggcg gttcttccag
    16381 ttgcaataat gtgtataatc gtcgcccctg atgatgtgaa ggtcaatgta tgcagtgcgc
    16441 actttacgac gcgggtcgct gtcgttcctg tcagtggata acgcagccga ttccagagca
    16501 actctccgct aaaaccgccg atcttaaaaa tctgctcgcc gattttccgg ttgaggaatg
    16561 gtgcgcgccg gtgtcaggcc cggaacaagg gtttcgtaat aaagccaaaa tggtggtgag
    16621 tggtagcgtt gaaaaaccac tgctcggtat gctgcatcgc gatggcacgc cggaagacct
    16681 ttgtgactgc ccgctttatc ctgcctcttt tgcgcccgtt tttgcggcgc taaaaccgtt
    16741 tatcgcccgt gcggggttaa cgccctacaa cgtggcgcgt aagcgtggcg aactgaaata
    16801 cattctgctg actgaaagcc agagcgatgg cggcatgatg ctgcgctttg tactgcgttc
    16861 tgaaaccaaa ctggcacaac tgcgtaaggc gctgccgtgg ttacaggaac aactaccgca
    16921 actgaaagtt attaccgtca atattcagcc ggtacatatg gcgattatgg aaggggagac
    16981 ggagatctac ctgactgaac aacaggcgct ggcagagcgt tttaatgacg taccgctgtg
    17041 gatccgtccg caaagtttct tccagactaa tccggcggtc gccagccagc tgtacgctac
    17101 cgcgcgcgac tgggtacggc agctgccagt taaacatatg tgggatctgt tctgcggtgt
    17161 ggggggcttt ggtttacact gcgcgacgcc tgacatgcag ttaaccggga tcgaaattgc
    17221 gccagaggcc attgcctgtg caaagcagtc agccgctgaa ctgggcttaa ctcgtttaca
    17281 atttcaggcg ctggactcca ctcagtttgc cactgctcag ggggaggtgc cggagctggt
    17341 gctggttaac ccgccgcgcc gcggcattgg taaaccgctg tgtgattatc tctcaacgat
    17401 ggcaccgcgt tttatcatct actccagctg taacgcccaa actatggcga aagatatccg
    17461 cgaactgcct gggtttcgca tcgaacgggt acagcttttt gatatgttcc cgcacaccgc
    17521 gcactatgaa gtgctgacgc tgctggtgaa gcaatctgaa gccgcaggtg cggcttcaga
    17581 ttgctgatca ggtgcgcttt gttcatgccg gatgcggcgt gtacgcctta tccggcctac
    17641 aaaatcatgc taaatcaata cattgcagag ttgatgtggg ccagataagc gcagcgcatc
    17701 aggcaatctt acttttgtca tcagtctcaa ttactgcggg aaccactggt cattgatttt
    17761 ctgatatgta ccgtcagctt taattgctgc cagcgcgtta ttcagttttt ccagcagagc
    17821 tttgttatcc ggacgtacag cgatgcccag accggtgccg aagtattgcg gatcggtcac
    17881 tttttcagta gcaacgccca gttgcggatt ggttttcagc cattcgttta ccaccgctgt
    17941 gtcaccaaat accccatcaa tacgaccatt tttcagatcg ataaaggcat tctgataact
    18001 gtcataggag acagttttca cttcggggtg ctgatcctga atatatttct ggtgcgtggt
    18061 gccgttttcc atcccaatac gtttgccttt cagatcggca aacgttttgt aggtatcttt
    18121 tttggcaatc acgacggctg agttttcata gtagggcgtg gtaaacgata cctgcttgct
    18181 acgctcgggc gtgatatcca taccggagat tacggcgtca tattttctga atttcagtga
    18241 cgggatcagg ctgtcgaacg cgtgattagt aaaagtacat tctgcctgca tttgtttgca
    18301 caaggctttt gccagatcga tatcaaagcc gacaatctca ttattagcac ctatagattc
    18361 aaagggtgga taggtggctg aaacgccaaa attgattttc tctgcggcag aagcaccgaa
    18421 agtaaaggaa gcaagtaaag cggcaagaac taactttttc atgatggaac tcccgtctgt
    18481 caatcttatg atttttggcc gtgtctgcgg catgggataa caatgccatc aagtgaattt
    18541 atatgcaata aatatgatta aataatttaa atgaaataaa aaacgacgga taacttagtg
    18601 agttgtccgt cttcattata agaatttatg cactatgtag gccggataag gcgtccacgc
    18661 cgcatccggc acaggcaccg tgctgatgtc tgatgcgacg ctggcgcgtc ttatcagacc
    18721 tacaaacccc ccggcgaatg tacgcagcta cattaattcc gccgttcgaa tgccagtgct
    18781 ttgcgctcga tcagacgcat catcagcgtc agcaggccgt tgacgaccag gtaaataatc
    18841 cctgccgcac cgaacaccat tacatcgtag gtgcgtccgt acaacaactg gctgtatccc
    18901 atcacttcca tcagcgtaat ggtgtaagcc agagaggtac ttttgaacac cagcaccact
    18961 tcgttggaat aagaagagag cgagcgttta aaggcatacg gcagcaggat cgccagcgta
    19021 tcttttttgc tcattcccag ggcgctacag gactgccact gaccttctgg gatcgcacga
    19081 attgcaccgt aaaacagctg cgtggtatac gccgcactgt tcagcgataa cgcaatcagc
    19141 gcacataacc acggttctga caacaaatgc cacagtgccg gatactcctg caaagtcgga
    19201 aactggcccg gcccgtaata aatcaggaag atctgcacca gcagcggcgt accggtaaac
    19261 agcgtgatat aaccccgcac cagccacacc agcaccggcg ttttcagcgt caggatgatg
    19321 gtaaaaatca atgccagaat cagtgccaca atcagcgagg caacggttag cgtcaggctg
    19381 gtgtgtagcc ctttcatcag ttcgggtaaa tactcaaaca ttagctgggc ctccgctcaa
    19441 aacgtgtcgc gcgcaggtca atgcgtttga gaatgtactg actgagcagg gtgatcacca
    19501 ggtaaatcgc cgccgccaca atataccagg taaatggttc ctgggtacga gtagcgatgc
    19561 tttttgtttg cagcattaaa tcattcacac taatcaaact gaccagcgcg gtatctttca
    19621 gcagcaccag ccactggtta ccgaggccag gcagcgcatg acgccacatc tgcggcatca
    19681 ccagacggaa aaagatagcc gatttcgaca gccccagcgc ctgaccagac tcccactgac
    19741 ccaccggcac cgctttcaac gcgccccgaa gcgtttgcga ggcataggcg gcatacagca
    19801 gtgacagagc gatgacacca caaaggaacg ggctaacgtc gaagttctca atgtccatct
    19861 gcactgggat ctgcacgaac ccaagattga tagtgaagcc atccgaaagc gtcagcagca
    19921 gctgcgagga gccaaaatag ataaacagca ccaccagaat ttctggcagg ccacgcagaa
    19981 tggttaccag cgctgaacct gcccacgcga caggacgcca tttttccgac tcccataccg
    20041 caaagaacat cgccagcgcc agcccgacaa tcaatgcaca aacggcaagg ccgacggtca
    20101 tcccggcggc gcttgctaaa ggaaaaaatt cattcatcag gaattacttc tggaaccatt
    20161 tgttgtagat ggtttcgtaa gtgccatctt tcttcacttt ttccagcgca gtgttgagtt
    20221 tctgctgcag ctcagtgttg ccctgacgta ccgcgatgcc gaggccagtg ccgaagtaat
    20281 ctttatcggt cactttgtcg cccaccgccg ccagtttcgg gttatctttc agccactcag
    20341 tgaccactgc ggtgtcaccg aagacgccgt cgatacgccc gttttgcaga tccagttttg
    20401 cgttctggta gctgttatac ggaacggtag tgatttccgg gtgcttatcc ataatgaatt
    20461 tctggtgtgt cgtcccgttc tgtacgccga cttttttgcc tttcagctga tcaacactgg
    20521 tgtatttgcc ttgctgaccc acaaacaggg cagagttgtc atagtacggg gtggtaaaca
    20581 gcacctgctt ttcacgctcc ggagtgatat ccatgcccgc catcacggct tctacgcgac
    20641 ggaatttcag gcttgggatc aggctgtcaa acgcctggtt agagaaagtg caggttgcat
    20701 caatctcttt acacagcgct tgtgccaggt cgacgtcaaa accaacgatc tggttgtttg
    20761 catcaatcga ttcaaacgga ggataggatg cttcggtagc aaaacgaatg gtttcggcag
    20821 ctgtggcgga aagactaaaa cctgcaatta acgcggcaat cagaactttt ttcattgttg
    20881 ttatcccgaa tcttagtgag agagatagtt tttaaatgct tcggtttgcg gctcggtaaa
    20941 gcagctcgcg tcgccttgtt ctacgatatg accattttcc atatacacca ctcggctggc
    21001 ggttttacgc gccacttcaa cttcgtgggt gacgatcacc tgggtaatat tcgtttctgc
    21061 cagctcacga atgatgctga cgatttgtgc cgtaatttcc gggtccagtg cggcggtcgg
    21121 ttcatcgaac agcagtacct gcggttccat catcaacgca cgggcaatag caacacgctg
    21181 ctgctgacca ccagaaagat gcagcgggta acgatcgcta taaggtttga gacgcagacg
    21241 ttccagcagt ttttctgcac gggccagcgc ctgatcttta ctcaacccca gtacacggca
    21301 gggcgcttca atcaggtttt gctgcacggt cagatgcggc cacaggttgt attgctgaaa
    21361 caccatgcca acgttacgac gcaaatcgcg aatcgctttg tcagagggtg ttttggtgaa
    21421 atcgaaatgg ttgcctgcaa tgttgagcgt accggagcgc ggcatctcaa gcagattgag
    21481 tacacgcagc agcgagcttt tacccgcgcc gctggggcca agtaacacca gcgtttcgcc
    21541 ctgtgggcaa tccagcgtga tatcgaacag cgcctgatgc gcgccgtaga agcaattaat
    21601 gccgtttaat tgaatactca ttgacactcg tatactggca gtctgatagc tattgaggtc
    21661 gaagatagta cctttgacag aataattatg caatatttct gctttaaaag ttaaaagcaa
    21721 agcgcattat tcaataaaca tagcacaaaa taacgggggc ggtggtcggc gagcataaat
    21781 gtcggcattc ctcacgaaat gccggacaat ttacggggtt tattggttga tcaaggcgtt
    21841 agcgattctc gatggactga cggagcgtac ccgccgtggc atgaacgctg ccgcctaagt
    21901 agcgtacatc gtcgatcacc caacactggc cttcctgaat cattaacact tcatcctgcc
    21961 agctctgatc accctgtttt agatccacac gcagaggaat atttcgcgca tcacgattcg
    22021 ggattgttga ggcactggca acacgggcgc tctctggcaa ggttgtgcga ctggagaatg
    22081 gatcgttggt cagtagttcc cgatggttat tatcccggga ggcatcgcta agcagtgtcg
    22141 ccagtttgtc gctcagataa gggcgcaagg cggtgatgtc gttgctgcgg tgcaaaatgc
    22201 ggtagtcata aaattgctgg gccacgttac ccgggcctcc ttcaacgcaa ggaccactgc
    22261 gtgtgccgtt atctttataa gctggagtga ctgtggtgca ggcactgagg agcagtgcgc
    22321 aggggataag cattgtcaat ttgctgtagc gcataatgat ttccttataa gcgatcgctc
    22381 tgaaagcgtt ctacgataat aatgatatcc tttcaataat agcgtatcag tctgataatg
    22441 cttttgagat cgaaggctta gcaaacaagg agatcgatca tgcaattttc tacaacccca
    22501 actctggaag gccagaccat cgttgaatat tgcggtgtgg tgaccggcga agcgatttta
    22561 ggtgccaata ttttccgtga tttctttgcc ggtatccgcg atatcgttgg cggacgttcc
    22621 ggtgcgtatg aaaaagaact gcgtaaagca cgggagatcg cctttgagga attaggctcc
    22681 caggcgcggg cgctgggggc cgatgccgtc gtcggtattg atatcgacta cgaaacggtc
    22741 gggcaaaacg gcagtatgct gatggttagc gtcagcggta cggcggtgaa aacgcgtcga
    22801 tgagaagagt cttctggctg gtcgctgccg ctctgttatt ggcagggtgt gcaggcgaaa
    22861 aaggcattgt cgagaaagag ggatatcagc ttgatacccg acgccaggcg caggcggcgt
    22921 atccgcgcat taaagtgctg gtgatccact acaccgcaga tgattttgat agctcgctgg
    22981 cgacactgac cgataagcag gtcagctcgc attatctggt ccctgcggta ccaccgcgat
    23041 acaacggtaa accgcgcatc tggcaactgg tgccggaaca agaactggcc tggcatgcgg
    23101 ggattagcgc ctggcgcggg gcaacgcgcc ttaacgacac ctctattggc attgagctgg
    23161 aaaaccgtgg ctggcaaaaa tcggccggag tgaaatattt tgccccgttt gaaccggcac
    23221 agattcaggc gcttattcca ctggcgaaag atattattgc ccgttatcac atcaagccgg
    23281 aaaacgtagt ggcacatgcg gatatcgcac cgcagcgcaa agacgatccg gggccattat
    23341 ttccctggca gcaactggcg cagcagggga ttggtgcctg gccggatgcg cagcgggtta
    23401 acttttacct tgccgggcgc gcgccgcaca caccggtaga taccgcgtca ttgctggagc
    23461 ttttggcgcg ctacggttat gacgttaaac ctgatatgac accgcgcgag cagcggcgcg
    23521 tgattatggc attccagatg catttccgcc cgacgttata taacggcgaa gcggatgcag
    23581 aaactcaggc gattgccgaa gcattgctgg agaaatacgg gcaggattag cgcggcagtt
    23641 ttccgtggtc gcgtagccag gcggcagttt tctcgatacc ttcatccagg gtgatgaccg
    23701 gctgataacc taactcttcc tgcgcacgcg taatatccag cgtaaagtca aaattcaact
    23761 tggagacgcc gtagtgggtc agcggcggct cttttgctga cttgcggaaa cgctccatgc
    23821 tgcgggcgat catatccagc atcgggtagg ggacggaacg aatacgacag tcaatattca
    23881 actcgtcgat cagcttctgc acgatgctgc acagtgtgcg atgctcgccg ttggtgatgt
    23941 tgtacacacg cccggaaggt agcttatcgc aggcttcctg gcttgccagc cacattgcgt
    24001 gcacggcatt ttcatagtag gtcatatcga ccagcgcact gccgccatgc ggtaacagaa
    24061 tactgccgta gtggtgcatc atatgcgcca gacggggaat aaagacttta tcgtgcggtc
    24121 cgaacagact ttgtgggcgc agaatagtaa agcgcgtttg tggattcgcc tgcgaaaaca
    24181 tattgatcac ttcttcgctg gctgctttgc tgcgggcaaa ctcgttggcg aagcggtgag
    24241 ggcgaaaatc ttctttaata tcgcgatggt ggtgataatc gaagtacagg gagggggaag
    24301 agatatgaat aaagttacgc acaccccagg cgacagccca ttcacccaag cggcgagtgg
    24361 cgcgaacgtt agccagatcg aaagcctgtt gtgtccccca gggtgaggta aagctggagc
    24421 agtgccacag cgtatcaatg cccgcgagca tcactttagc ttgtgatgaa accagctcgg
    24481 tcagatccgc cggaacaaac tctgcgccca ttttttccag caatttgccc attgcctcgt
    24541 tgcgaccggt cgctcgcacg ctgatgcctt tctggcataa aaactctacc gcgtttcgac
    24601 ctaagccgct ggtggcgccg gtaaccagta ccttcatatc aatccactgt tgttgagaaa
    24661 ataacgtgcg cattcttccg tgatttcccc catgatgcaa tgggaaacat gaaagaataa
    24721 cgcaggtttt gtcgattaat ctgtgctttg ttctgccagt ctggcgattt gttttgccat
    24781 tccgcgaaaa ataaacagat gcgcggggat catcaataac cagtaaaaca gccccggcat
    24841 accgtgcgga tgccagaaag cgcggacatc gatagtacga tagtcgcctt tatcttccag
    24901 gctaaaacac agtcgtccca gccccggcgc tttcatgcca aataacaacg taagttgttt
    24961 ttccggttca acgacaatca ctttccagct atctaccgca tcgccagtct gtaaatattc
    25021 gcgctccggg cggcctttcg ccagcttatg accgatcgcg cggtccatca acgcccgtgt
    25081 ctgccacaaa atattgccaa agaaataacg ctctttaccg ccgatttggt tcactacctg
    25141 ccataaagca gcaaggctgg cggacgtttt aacggtaaac cccgcctgtt tggcaaaata
    25201 accgtactcc ggtcgccagc gggcaaaggc ctgagcgtcg tagccccagt cgctggagtt
    25261 gaccagtttt tcctcctctt tcaacgtgct acgtaccgcg tcatcgaaag cgatcagccg
    25321 ttgtgggatg agtgcacgta gcgcggtatc atccgccagc agatcgtgtt tcagcccctg
    25381 aatcaacgcc ctggcggtgg tgggcggtac ggaagtaatc acattgagaa accacaccga
    25441 aatccagcgg gtggggaggg ggatggggat caaccagcgg cgcttgccgc tcaccgccat
    25501 aaaatgttca aactgttgct gataactgag cacctctggt ccggcggctt cgaagatgcg
    25561 gtgttcgctg gccggatgat ctaacaacgc caccagatag tgcagcaagt tttccagcgc
    25621 gatgggcgtg gtgcgtgaac gtacccagcg tggcggcgtt aacactggca ggttgtagac
    25681 catatcgcgc atgacttcga acgccgctga acctgcgcca acgataattc cggctcgaag
    25741 ttcggtcaca ggtacattcg cttcacgaag aatgtccgcc gtagcctgac gagcacgcag
    25801 atgatccgac tgctcatgtg gcggggcctg caacgaactg agaaagatta attgcttaac
    25861 tggtacttca cgtagcgcat cgcggacgtt gagagccacc tggcgctcct gagcgataaa
    25921 atcgccgcct tcgcccatgc tgtgcaccag aaaatagacg gtatcgatat cctgcaacag
    25981 ggccggaagg ttatccggcc agctgagatc gactttatgg caactgacgt ttgccagttg
    26041 cagctttgca agcctgtcga catgacgtgc cgccgccagg atctgatgcc cttgctggct
    26101 gagtgtgcgc accagatgct gaccaatgta gccactggca ccgagaacta aaatgcgttg
    26161 cggcacgtct ctctccttaa cgcgccagga atgcgcgcca gtgggcggcg acttccgcca
    26221 gttgttcgcg cgagacgtca agatgcgtca ccaggcggac aatcggcgag gcgttaatca
    26281 gcacgtttct cactttcatg tattcgccta acgcggcagc attttcttcc ccgacgcgaa
    26341 caaacagcat attagtgtcc tgacgcatca catccgcgcc tgcttcacgc agttgctccg
    26401 ccatccaggc ggcgttgtcg tgatcttcct gcaaccgtgc gacgttattt ttcagggcat
    26461 acatcccggc ggcagccaga atcccggact gacgcatccc gccaccggcc attttccgcc
    26521 agcggatggc acgtttaatg taatcacgat taccgacgag taatgaaccg acaggcgtcc
    26581 caagaccttt cgacaggcaa atggtgaacg aatcacaata ttgcgtgatc tctttcagtt
    26641 cgcagccgta agccaccacg gcattaaaga tgcgcgcacc gtcaacatgc agcgccagat
    26701 tgcgctcgcg ggtaaattcc catgcttctt tcaggtattc acgcggcagc actttgccgt
    26761 tgtgggtgtt ttccagactg agtaatttgg tgcgggcgaa atggatatcg tcgggtttga
    26821 ttttcatcgc cactttatcc agcggtagcg tgccgtcggc agccgcgtcg atgggttgcg
    26881 gctgaatgct gcccagcacc gctgcgccac cggcttcaaa cagatagtta tgcgcggcct
    26941 gaccgacaat atactcttcg ccacgttcgc agtgactgag cagagcgacc aggttggcct
    27001 gagtgccggt aggcagaaaa atggcggctt ctttaccgga aagctctgct gcatagtcct
    27061 gcagagcatt aacggtaggg tcgtctccgt aaacgtcgtc cccaaccggg gcggccatca
    27121 tcgcttcgag catggcgcgg ctcggtcggg taacggtatc actgcgtaaa tcaatcatgg
    27181 catgtcctta ttatgacggg aaatgccacc ctttttacct tagccagttc gttttcgcca
    27241 gttcgatcac ttcatcgccg cgtccgctga tgattgcgcg caacatatac aagctaaatc
    27301 ctttggcctg ttcgagtttg atctgcggtg gaatggctaa ctcttctttg gcgaccacca
    27361 catccaccag caccggaccg tcgatggaga aggcgcgttg cagggcttca tcaacttcag
    27421 acgctttttc tacacggata cccgtaatgc cgcacgcttc ggcaatgcgg gcaaagtttg
    27481 tgtcgtgtag ttcggtgccg tcagtcaaat agccaccagc tttcatctcc atcgccacaa
    27541 agcccagcac gctgttgtta aagacgacaa ttttcactgg cagtttcatc tgcactactg
    27601 agaggaaatc gcccatcaac atgctaaaac cgccatcgcc gcacatggcg accacctgac
    27661 gttctggctc tgtcgcctgc gcacccagcg cctgcggcat ggcgttagcc atcgaaccgt
    27721 ggttaaacga acctaacagg cgacgcttgc cgttcatttt tagataacgt gccgcccaca
    27781 ccgttggcgt accaacgtca caggtgaaaa tagcgtcatc ggcggcaaaa tgactaattt
    27841 gctgcgccag atattgcggg tgaatggctt tctcgctcgg tttagctaaa tcgtccagcc
    27901 ctttgcgggc gtcgcggtaa tcttccagcg ctttatccag aaacttgcga tcggcttttt
    27961 cttccaccaa tggaagcaat gcacgcagag tcgacttgat atcgccgacc agtgccatat
    28021 ccaccttgct gtgagcgccg atgctggctg ggttgatatc aatctgaatg attttggcat
    28081 cggtcgggta gaaggcgcgg tagggaaatt gcgtgccgag tagcactaac gtgtcggcgt
    28141 tcatcatggt atggaaacct gacgagaagc cgattaaccc ggtcattcca acatcatacg
    28201 gattatcgta ttcgacatgt tctttaccgc gcagggcatg aacaataggc gctttaattt
    28261 tcccggcaaa ctcaactaac tctttatgcg cccccgcgca gccgctgcca cacatcaggg
    28321 cgatattgct ggaataacgc agcagttgcg ccagtttgcg taactcttct tcttccggcg
    28381 tcacgactgg ttgtggcgca tgataccagt gcgtggttgc cccttctggc gcaggtttta
    28441 acgccacgtc gcctggtaac acgacaaccg aaacgccacg gttaagcacc gctttgcgca
    28501 tggcaatcgc cagtacttgt gggatctgct ccgggctgga aaccagctcg caatagtgac
    28561 tacattcgcg gaatagctct tgtgggtggg tttcctggaa atagccgctg ccaatttcgc
    28621 tggagggaat atgagcggca atcgccagta ccggaacgtg attgcggtgg caatcaaaca
    28681 ggccgttgat taagtgcagg ttgccggggc cgcacgatcc ggcgcagacc gccagttctc
    28741 cgctaagttg tgcttcagcg ccagcggcaa aggccgccac ttcttcgtgg cgggtggaca
    28801 tccactcgat ggtgcccatg cgattaagac tgtcactaag accattcaga gagtcgcctg
    28861 tgactcccca gatgcgtttc acccctgccg attcgagtgt tttggcgata taagctgcaa
    28921 ccgtttgttt catggttctc catctcctga atgtgataac ggtaacaagt ttagttcatc
    28981 tgacggaggg ggaagggatg ggagagaaag gaggcactaa cggttaaata gcccgatgaa
    29041 aggaatatca tcgggcataa ggcgattatg cgagaaccaa atccccctgc ggatggcagg
    29101 agcaggccag tacgtaacct tcagcgattt cggcgtcggt cagcgtcatt gtactgctca
    29161 ccgtatattc accggaaacc acttttgtct tacagcagcc gcaaacaccc gcacggcagg
    29221 cagcgacaac cggaacgtta ttgctttcca gcgcctccag tagcgtggtg ccaaccgggg
    29281 cgtaaaattc tcgtgccggt tgcagtttgg tgaatttcag accgctggtc gccgcttttg
    29341 ctactggggt gaagaatttc tctttaaaga aacgcgtcac gccgagcgct ttcacttcct
    29401 gctctaccca atccatatac ggagccgggc cgcaggtcat cacggtacgt gaagctaagt
    29461 caggtacacc tgccagcagt tctcgagtga gacgaccagc gataaagcct tcggtaacgt
    29521 tattttctgc caccagcgtt accggatagt tacgccactc atcggcgaaa ataacatcct
    29581 gcggcgtatg cacgttgtag atcacccgca catcggcctg tggacggttc ttcgcaagcc
    29641 agcgacgcat cgacataatc ggcgtgacgc cgcagcctgc cgccagcaac aggaatttat
    29701 cttctgcttt atcgtcgcag gtaaattccc ccatcgcgtc cgaaagccag agataatcac
    29761 cgcgttttac atcgcgcgtc agccactggg agccgacacc gtcatcaatc cgccgcacgg
    29821 tcagggtgat atattcactc acgcctggcg tggaggaaat ggtgtaagca cgcagcgttt
    29881 ccgctgagtt tcgcacgctg accagtgcat attgcccggc gcgatatggg tagtaatcgt
    29941 ggcaaatcag ggaaatcgtc cacacatccg gcgtttcttg cgtaatgtga tgaacctgca
    30001 tccgccacgg gcattgattc gttggcatcg tcatcgacaa actccttacg cgctcaacag
    30061 ttgcttcatg tcttcttcaa cagtggtgat agaacgcagg ccgaatttct cgttcagcac
    30121 cgccagcagg tctggtgtca ggaaaccagg tgcagtcggg ccggtgacga tatttttcac
    30181 gcccagagaa agcagcgtca gcagaatgac gatcgctttc tgttcaaacc aggagagcac
    30241 cagcgacagc ggcagatcgt tcacaccaca gcccagttta tctgccagag tgacagccag
    30301 aataatcgct gagtaagcat cgttacattg acctgcatct accagacgcg gcagaccttc
    30361 gatatcgcca aactcaagtt tgttaaagcg atatttacca caggcgaggg tcaggatcag
    30421 gcagtcatcc ggcacgatgg tggcgaaatc ggtgaagtag tggcgctcgc cgcgtgcgcc
    30481 gtcacagcca ccaagcagga agatatgacg cagtttttca cggctcacca gatcaatcag
    30541 cgtatcagca gcgccaagca gcgtctggcg accaaaaccc acggtgataa ggtgcggaat
    30601 ttcgctgtac gggaagcctg ccatctgttg cgcctgggtg ataaccgcag agaaatcatc
    30661 accgtccaga tgacgcacgc caggccagcc aacaatgctg cgggtccaga tacgatcgtc
    30721 ataagcgctt acggttgggt cgatgatgca gttcgaggtc atcacgatgg ggccagggaa
    30781 acgagcgaac tccacttgct gattctgcca gccgctgccg tagttaccga ccagatgctt
    30841 gaatttacgc agctccgggt agccatgcgc aggcagcatt tcgccgtggg tgtagacatt
    30901 aacgcccgtg ccttcggtct gttccagcag gttgtagaga tctttgagat cgtgaccgga
    30961 aatcagaatg catttacccg ccgtcgcttt gacgttgacc tgggttggcg tcgggtgacc
    31021 gtatttaccg gtttcgcctg catccagaat gctcatcact ttgaagttca tctggccgat
    31081 ttccattgaa cactcaagaa gcgcgttcat atcggcaggc caggtcccca gccacgccat
    31141 gattttatgg tactgggcat aaatatcgtt gtcgtattga ccgagaacat gcgcgtgttc
    31201 catataggcc gccgcacctt tcaggccata caggcacagc agacgcaggc cgagaatgtt
    31261 ttcgccaatc gccgctttat ctttgttagg ggtaaattct gctgcctgac gttgcagctc
    31321 gccgagatca tcgctcacca gttgcaggtc agccatcggg ttatcgacgc gcgcgttggc
    31381 atctacagcc aggcattgtg ctttcagcgc ctcgcgcaag gcaatcgctt cacgagcgta
    31441 gccgacaata cgcggagaat cgaagttaac gttggtcagg gttgagaaaa aggcacgtgg
    31501 cgcgaagctg tcaacatcgt ggttgatgat gccgtattca cgcgctttta ccgcccaggc
    31561 agaaagccct tgcagcgccg cgatgagtaa atcctgaagg tcagaagttt ccgccgtttt
    31621 accacacatc ccctgcgcgt atgagcagcc gtttcctgcc ggagtacgga tagtttgttc
    31681 acattgcaca caaaacatga tcacaccttt taaagttata tttaatatac atgtttaagg
    31741 ttaagacgct taacgcgggg ataaaaggga tttttcatgc aactttaagg gagattgatt
    31801 tagcgcaatt ttggcggcag ggatctaccg ccagagaggt attacgcaga gaaaaaggcg
    31861 atgaggatcg gcactaacag gctaagaata aaaccgtgaa caattgccgc cgggaccata
    31921 tccagcccgc cagtacgttg aagaacgggc agggtgaaat ccattgatgt ggcaccgcat
    31981 aagcccagtg cagtagagcg gctgcggcga atcagcccag ggatcaacat aatagcaatc
    32041 agttcacggg ccagatcatt aaaaaacgcc gcgctcccga ttaccggacc aaaagattcg
    32101 gtcaataaaa taccggaaag agaataccag ccgaaaccgg aggccattgc cagcgcggta
    32161 ttgatgggga gatcaagaat aaaggcgtta attaaaccac caattaatga actgacaacc
    32221 accaccacgg cgacaatcat tccccggcga ttaaggacaa tctgctttaa ggtcatgcca
    32281 ttattgcgca actgaatacc aacgaggaaa agtagcaaaa ttaacgtgta ttcactggct
    32341 tcggtcgcgt gttgtaagaa agccagtcca ctcagaccaa tggcaaaacc aatcactact
    32401 acgccgcaca gttttagcga ctccagcgcc atcgcaatac gcgacgggag tttttcttgc
    32461 tgatggtggt tgcgccacgg caggcctcgc tccagccaca tcagggcggc aatattacac
    32521 agtaaaataa cggtaatact gacggcagaa taatgcagaa tcgccaacag gttactggcg
    32581 aggttatcga gaaacgccag actgataccc ataaaaaaga gaataaggta aaccatccag
    32641 cttaatagct gattaataac ttttaacgca gcttgttggc gaagcggaat gaggtaaccc
    32701 acaatcaggg gaaccagaat gattaacagc ccagaaaaca tgaaaaccca gtccttgcaa
    32761 agatgaagtc gaaatgcgcg atgacacact actgaaagcg gaaggacgag taaagttgca
    32821 attaaaagga aatgttatgc ataaggagca gtagagtatt cgttttcatt taaagatatt
    32881 cttgcgcttt aattacaaac tgcaccgatg ttggtggcgt caaaatcgcc gaggcgttcc
    32941 ctgaaggccg gggcagccca catggatgtg ggctgagggc gcgttttaca gggatgttac
    33001 ctcgcgcccg acccggtagc cgtaagggat aagtcgaggg caccgcgcag cggcgatttt
    33061 gttcgccaga gcccgggggg gcagggggcg gcggcgattg gccgccccct gcgcgctcct
    33121 tgcgccagtg gcaatatgtg gcttagctca tgaaaggagc gcaacaagat gatgaatcaa
    33181 catataacaa catcttaaaa aaagcctgac attacgccag gccttctgcg ttaattaatc
    33241 acgcttttcc agcagggtcc ggtaaatcag accaccgata atgccgccga caattggcac
    33301 cacccagaag aaccacagtt gttctaatgc ccagccgccc tggaagatag caaccgcggt
    33361 gctgcgcgcc gggttaacag aagtgttagt caccggaata ctaattaagt gaatcagggt
    33421 taaggccaga ccaatagcga tcggcgcaaa acctgccggc gcgaatttgg cggttgcgcc
    33481 gtggatcacc aacaggaaac ctgcactcaa taccagttca actaccagcg cggaaagcat
    33541 ggaataaccg cctggtgaat gctcgccata accgttagaa gcaaaaccgc tggctgccgc
    33601 gtcaaaaccc gttttaccac tggcaattaa atacagcagc gccgctgcaa caataccgcc
    33661 gacaacctgg gcaattacgt agccaacgac ttcttttgcc ggaaaacgtc cgccagccca
    33721 taaaccaata gtgaccgccg ggttaaaatg accaccagaa atatgaccaa cagcaaaggc
    33781 catcgtcaga acggtcagac cgaacgccaa cgccacgccg gcaaaaccaa tgcctaattc
    33841 cgggaagcct gcggccagta cagcactacc acagccacca aaaacaagcc agaaagtacc
    33901 aaaacattca gctgctaatt ttctgaacat atccacctca attaaaaatt gaccctgtga
    33961 aaaatatggt cgttttatag ggtcgtcgta aaaaaatgac gacggaaata atgcgcggct
    34021 attttaaaaa cgaaggcgag tcattcacca gataaataaa tccagtaaat ttgatttagg
    34081 gcaacagcgg gttgccccat atagtcattt gtctgattga cagtgtagtg cacgcaaaag
    34141 atttaatcct ttaggcgtaa taaaaaataa tatatcatgc taattatttg attttgttgt
    34201 ttttgcagac ttatcagcaa gagggagtat aacgcgatta ttcgctcatt tttcagacat
    34261 ttgccatgct taaatgtgat gtcatcacgt attagcaagg cctttcccgt tatactgcca
    34321 gcgtaaagga taagtcacat atttctggag gggatatgat tcttgagcgc gttgaaattg
    34381 tgggttttcg cggtatcaac cgtttgtcgt tgatgctgga acaaaacaac gtcctgattg
    34441 gggagaacgc gtggggtaaa tccagcttgc tggacgcctt aaccctgctg ctatcgccag
    34501 aatcagatct ctaccatttt gagcgcgacg atttctggtt cccgccggga gatatcaacg
    34561 ggcgagaaca tcatctgcat attattttga ccttccgcga atcgctgcca ggccgacatc
    34621 gggttcgccg ttatcggccg ctggaagcgt gctggacgcc atgcaccgat ggctatcacc
    34681 gtatttttta tcgtctggaa ggggagagtg cggaagacgg cagcgtgatg acactgcgca
    34741 gttttctcga taaagacgga catccgattg atgtcgagga tattaacgat caggcacgcc
    34801 atctggtgcg tttaatgccg gtgctgcgct tgcgtgatgc ccgttttatg cgccgtattc
    34861 gtaacggcac ggtgccaaat gtccctaatg tggaagtcac cgcgcgccag ctcgatttcc
    34921 tcgcccgtga gttatcctca catccgcaaa atctctctga tgggcagatt cgtcagggac
    34981 tttccgcaat ggtacagctg cttgagcatt atttctctga gcagggggcc ggacaggcgc
    35041 gatatcgttt aatgcggcgg cgggccagca atgagcaacg aagctggcgc tatctggaca
    35101 tcatcaaccg gatgattgac cgacctggag ggcgctcgta tcgggttatt ttgctcggcc
    35161 tgtttgctac tttattgcag gcaaaaggca cattgagact ggataaagac gcgcgtccat
    35221 tgttgctgat cgaagatcca gaaacccgtt tacaccccat tatgctttca gttgcctggc
    35281 atctgttgaa tcttctgcca ttgcagcgca ttgccaccac caactcgggt gagttgcttt
    35341 cgttaacgcc ggtagagcat gtttgccgac tggtacgtga gtcctcgcgc gttgccgcct
    35401 ggcgtctggg gccgagtggc ttgagtaccg aagatagccg acgcatatcc tttcacattc
    35461 gttttaaccg tccgtcatcg ctgtttgcac gctgctggtt gctggtggaa ggggaaacgg
    35521 aaacctgggt tatcaatgaa ctggcgcgtc agtgcggaca tcattttgat gccgaaggga
    35581 tcaaggtcat tgagtttgcc cagtccgggc taaagccact ggttaaattt gcccgccgaa
    35641 tggggattga atggcatgta ctggtcgatg gcgatgaagc agggaagaaa tatgccgcta
    35701 cggtacgcag cctgttgaat aacgatcggg aagccgaacg agaacattta acggcgttac
    35761 cggcgctgga tatggaacat tttatgtatc gccagggatt ttccgatgtg ttccaccgcg
    35821 tggcgcaaat cccggaaaat gtaccgatga atctacgcaa aattatctcg aaagcgatcc
    35881 atcgctcttc caaacccgat cttgccattg aagtggcaat agaggcagga cgtcgtggtg
    35941 tggactccgt accgacgctg ctgaaaaaaa tgttctcacg cgtgctgtgg ctggcgcgcg
    36001 gtcgcgcgga ttaaccgcga aacatcgtgg ccatttgtgg ctgaatagcg tcgagcatct
    36061 catagcgccg acggtattca gcccgttttt tactggcgat ttcggcaatc tcttttcgtg
    36121 ctatctgtgc tggaaggcgg taatggcgtt cagcatcaca tacgccgcca accgattccc
    36181 agaaagcgtt gtaatcagcg tggatcttgc cttctttatc gcgataacgc aggctgcggt
    36241 aaatatgcgt ttcattgctg acggcaataa tctgctctac ctgcaaacgt tgggcaaaca
    36301 gacaggccgc ttccatcacg aggcgtttgg gaaatagccc gtggcaggct ttcgtcgcat
    36361 tctggatttc ctgatgtgga atttcccatt ttgcgccttg cagtccgcca ataaacatcg
    36421 ttcttttccc ctgatattca cacagggtaa acgtgatctc tgccagagga ataccttcgc
    36481 tgttgcgaaa caggattgtg ctgtcacctt ctttatccat tgagatcatc atggtcagct
    36541 taagcgtgaa ctgctcgccg tttttgcctt ccagcttcgc cagttgcagc ccgggggtat
    36601 tcaaatataa gctgaattct tccgccgaca tacatccgcg gagtaacgca taatggtaac
    36661 gtaacgcctc cagcaattgc ttacggctaa gattcgccgc aaggtaaggg cgatgcagac
    36721 gcacaggcag tcgcggctgg cgcgttaaca acacattgag attaggccag tgggaaagtt
    36781 cgttcatcca ctcaacgctt aaacgcggca taatcaacga gcgcagcaaa aatttctggc
    36841 gaaaactacg gcgatgccag aatttacccg gccgacactg tccacgtgcc agactaagaa
    36901 aaagtgacag gctgctgaga gattcagatg gcgtaaaggt ccgttcagtt agctgcgaca
    36961 tattcatgaa atcattggtt atacatgacg tcgatttcac cattgcgtat cttaaccaaa
    37021 catcaatagt gtgattacta acgtaaattt tagggttttg ttgatatttc gttgaagtta
    37081 atgacccgga ttggcatatg gagtattcag aatatttatg aaaaagcgga aaaccgtgaa
    37141 gaagcgttac gttattgcgc tggtgatagt catcgccgga ctgattacgt tatggagaat
    37201 tcttaacgca cccgtgccga cttatcagac actgattgtg cgccccggtg atttacagca
    37261 aagcgtgctg gcgaccggaa agctggacgc gctgcgtaag gttgatgtgg gcgcgcaggt
    37321 cagcggtcag ttgaaaactc tgtcggtggc gattggcgat aaagtaaaaa aagaccagct
    37381 tttaggggtt attgatcctg aacaggctga aaaccagatt aaggaggtcg aagcaacgct
    37441 gatggagcta cgtgcgcagc ggcagcaggc ggaagcggag ctgaaactgg cgcgggtgac
    37501 gtattcccgt cagcaacgtc tggcacaaac gcaggcggtt tcacagcagg atctcgacac
    37561 cgccgcgacg gagatggctg tgaaacaggc gcaaattggc accattgacg cggaaatcaa
    37621 gcgcaatcag gcttctctcg atacggctaa aaccaatctc gattacactc gcatcgttgc
    37681 cccgatggcc ggggaagtca cgcaaatcac cactctgcaa ggccagacgg tgattgccgc
    37741 acaacaagca ccgaacattc tgacgctggc agatatgagc accatgctgg taaaagcgca
    37801 ggtttctgaa gcggatgtaa tccacctgaa gccggggcaa aaagcttggt ttacggtgct
    37861 tggcgatcca ctgacgcgct acgaggggca aatcaaggat gtactaccga cgccggaaaa
    37921 ggttaacgac gctattttct attacgcccg ttttgaagtc cccaacccca atggtttgct
    37981 gcggctggat atgactgcgc aagtgcatat tcagctcacc gatgtgaaaa atgtgctgac
    38041 gatccctctg tcggcgttag gcgatccggt tggcgataat cgttataaag tcaaattgtt
    38101 gcgtaatggt gaaacacgcg agcgtgaagt gacgattggc gcacgtaacg ataccgatgt
    38161 tgagattgtc aaagggctgg aagcgggcga tgaagtggtg attggtgagg ccaaaccagg
    38221 agctgcacaa tgacgccttt gctcgaatta aaggatattc gtcgcagcta tcctgccggt
    38281 gatgagcagg ttgaggtgct gaagggcatc accctcgata tttatgccgg tgagatggta
    38341 gcgattgttg gcgcttcagg ttccggtaaa tcgaccctga tgaatattct cggctgtctg
    38401 gataaggcca ccagcggcac ctatcgcgtc gccggtcagg atgttgccac actggacgcc
    38461 gatgcgctgg cgcaactgcg ccgcgagcat ttcggcttta ttttccagcg ttaccattta
    38521 ctttcgcatt taaccgccga gcagaacgtt gaagtacccg ccgtctatgc cggtcttgag
    38581 cggaaacagc ggctgcttcg cgcccaggag ttactgcaac gactggggct ggaagaccgt
    38641 acagagtatt atccggcaca gctttcgggt ggtcagcaac agcgcgtcag catcgcgcgg
    38701 gcattgatga acggcggtca ggtaattctt gccgatgaac caaccggcgc actggacagc
    38761 cattctggcg aagaggtgat ggcgatcctg catcagctgc gcgatcgcgg gcatatggtg
    38821 attatcgtca cccacgatcc gcaggtcgct gctcaggccg agcgggtgat cgaaattcgc
    38881 gacggcgaaa ttgtgcgcaa tcctcccgcc attgaaaaag tgaatgttgc tggcgggacg
    38941 gagcctgttg tcaacacggt gtctggctgg cggcagtttg tcagcggttt taacgaggcg
    39001 ctgacgatgg catggcgggc gctggcagcg aataaaatgc gtactttact gaccatgctg
    39061 gggattatta tcggtattgc gtcggtggtt tccattgtcg tggtgggtga cgccgccaaa
    39121 caaatggtgc tggcggatat tcgttctatt ggtacgaata ctattgatgt ctatcccggg
    39181 aaagattttg gcgatgacga tccgcaatat cagcaggcgc tgaagtacga cgacttaatc
    39241 gccatccaaa aacaaccgtg ggtcgcctca gccacacctg ccgtctcgca aaacctgcgc
    39301 ctgcgttata acaatgttga tgttgctgcc agtgccaatg gcgtgagcgg cgattatttt
    39361 aatgtctatg gcatgacctt cagtgaagga aacaccttta atcaggagca gctgaacggt
    39421 cgtgcgcagg tcgtggttct cgacagtaat actcgccgcc agcttttccc ccataaagca
    39481 gatgtggttg gcgaggtgat tctggtcggc aatatgcccg ccagagtcat tggtgtggcg
    39541 gaagaaaaac agtcgatgtt tggtagcagt aaagtgctgc gtgtctggct accttacagc
    39601 acgatgtccg ggcgagttat gggccagtcg tggctgaact ccattactgt cagggtaaaa
    39661 gaaggatttg acagcgccga ggcggaacag caactcacgc gtttactttc actgcgccac
    39721 ggaaagaagg atttctttac ctggaacatg gacggcgtct tgaaaactgt tgaaaagacc
    39781 acacgtactt tacaactgtt tctgacgctg gtggcggtga tttcgctggt ggtgggcggt
    39841 attggtgtaa tgaatattat gctggtgtca gtgaccgagc ggacgcggga aattggcatt
    39901 cgcatggctg taggtgcgcg agcaagcgat gttttgcaac agttcctgat cgaagccgta
    39961 ctggtttgct tggtcggtgg cgcgttggga ataacgctgt cactgttaat tgctttcacc
    40021 ttgcagcttt tcttacccgg ctgggagatt ggtttttcac cgttggcact gctgctggcg
    40081 tttctctgct cgacggccac cgggatttta tttggatggt tacccgcacg aaatgcggca
    40141 cgactggatc cagtagatgc tctggtacga gagtaatttt tgagataaaa atgccagccg
    40201 atcgggctgg cattttgcct ttaggatgta cacaatgaga cagaagagct atgcgactgc
    40261 cgcttctact tcgacgggca caataacact ggcgtgattg ccttttggcc cctggtggac
    40321 atcaaactga acggattgtc cagcttttag cgttctgtaa ccatccatct gaatggtgga
    40381 ataatgagcg aaaatatctt cgccgccgcc ttcagggcag atgaaaccaa accctttggc
    40441 attgttgaac cacttaacag tacccttttc catgcttcga catccttcgc aaatcttata
    40501 caagtaagat ggaataaacc ggggtcagag agggggctgt tcaaaacctc gccaactcta
    40561 gaaatacaat ttagagaatt agggcgagcc gtcaagcgtt tgacaggggc cagggggcag
    40621 gtatgaatca aaaatttgaa gcagttaacg ctattgacag gaatgtgaca gatgtcgctg
    40681 atgccaacga tagatgatag ttatctatca tgtggagtag attggtcagg caaataagct
    40741 cttgtcagcg gcagggcgtt ctgccgataa ccgtaaccga agatgataac tgacaatggg
    40801 taaaacgaac gactggctgg actttgatca actggcggaa gaaaaagttc gcgacgcgct
    40861 aaaaccgcca tctatgtata aagtgatatt agtcaatgat gattacactc cgatggagtt
    40921 tgttattgac gtgttacaaa aattcttttc ttatgatgta gaacgtgcaa cgcaattgat
    40981 gctcgctgtt cactaccagg ggaaggccat ttgcggagtc tttaccgccg aggttgcaga
    41041 aaccaaagtg gcgatggtga acaagtacgc gagggagaat gagcatccat tgctgtgtac
    41101 gctagaaaaa gcctgaatgc aggcataaaa attgggggag gtgcctatgc tcaatcaaga
    41161 actggaactc agtttaaata tggctttcgc cagagcgcgc gagcaccgtc atgagtttat
    41221 gaccgtcgag cacttgttac tggcgctgct cagtaaccca tctgcccggg aggcgctgga
    41281 agcgtgttct gtggatttgg ttgcgctccg tcaggaactg gaagccttta ttgaacaaac
    41341 cacacccgtt ctgcctgcca gtgaagagga gcgcgacaca cagccgacgc tgagttttca
    41401 gcgtgtactg caacgtgcgg tcttccatgt ccagtcctcc ggtcgcaatg aggtaaccgg
    41461 tgcaaacgtt ctggtcgcta tctttagcga acaggagtcg caggcggcat atctgttgcg
    41521 taaacatgaa gtcagccgtc tcgatgtggt gaactttatc tctcatggca cgcgtaaaga
    41581 cgagccgaca cagtcttctg atcctggcag ccagccaaac agcgaagaac aagctggtgg
    41641 ggaggaacgt atggagaatt tcacgacgaa cctgaatcag cttgcgcgcg tgggcggaat
    41701 cgacccactg attggtcgtg agaaggagct ggagcgtgct attcaggttc tctgccgtcg
    41761 ccgtaaaaac aacccgctgc tggtggggga atctggtgtc ggtaaaaccg cgattgcgga
    41821 aggtcttgcc tggcgaattg ttcagggcga tgtgccggaa gtgatggctg actgtacgat
    41881 ttactctctc gatatcggtt ctctgttagc gggcactaaa tatcgcggcg actttgaaaa
    41941 acgttttaaa gcgttgctca agcagctgga gcaggacact aacagcatcc tgtttattga
    42001 tgagatccac accattatcg gtgcgggtgc agcgtctggt ggccaggtcg atgcggctaa
    42061 cctgatcaaa ccgttgctct ccagcggtaa aattcgcgta attggttcga caacctatca
    42121 ggagttcagc aacattttcg agaaagaccg tgctctggcg cgtcgcttcc agaaaattga
    42181 tattactgaa ccgtcgatcg aagaaactgt tcaaatcatc aatggcctga aaccgaagta
    42241 tgaagcgcac cacgacgtgc gttataccgc aaaagcggtg cgtgcagcgg tagagctggc
    42301 ggtgaaatac attaacgatc gtcatctgcc ggataaagcc attgacgtta tcgacgaagc
    42361 gggcgctcgc gcacgcctga tgccggtaag caaacgcaag aaaaccgtta atgtggcgga
    42421 tattgagtcc gtggtggccc gtattgcgcg cattccagag aagagtgttt ctcagagtga
    42481 ccgcgatacc ctgaaaaacc tcggcgatcg cctgaaaatg ctggtcttcg gtcaggataa
    42541 agccattgag gcgctgactg aagccattaa gatggcgcgt gcaggtttag gtcacgaaca
    42601 taaaccggtt ggttcgttcc tgtttgccgg ccctaccggg gtcgggaaaa cagaggtgac
    42661 ggtacagctt tcgaaagcgt tgggcattga gctgctgcgc tttgatatgt ccgagtatat
    42721 ggaacgccat accgtcagcc gtctgattgg tgcgcctccg ggatacgttg gttttgatca
    42781 gggaggtttg ctgactgatg cggtcatcaa gcatccacat gcggtgctgt tgctggacga
    42841 aatcgagaaa gcgcatccgg acgtgttcaa tattctgttg caggtgatgg acaacggtac
    42901 gctgaccgat aacaacggac gcaaagcgga cttccgtaac gtggtgctgg tgatgaccac
    42961 caacgccggg gtacgtgaaa ctgagcgtaa atccattggt cttatccacc aggataacag
    43021 caccgatgcg atggaagaga tcaagaagat ctttacaccg gaattccgta accgtctcga
    43081 caacattatc tggttcgatc atctgtcaac tgacgtgatc catcaggtgg tggataaatt
    43141 catcgtcgag ttgcaggttc agctggatca gaaaggtgtt tctctggaag tgagccagga
    43201 agcgcgtaac tggctggccg agaaaggtta cgaccgggca atgggcgcac gtccgatggc
    43261 gcgtgtcatc caggacaacc tgaaaaaacc gctcgccaac gaactgctgt ttggttcgct
    43321 ggtggacggc ggtcaggtca ccgtcgcgct ggataaagag aaaaatgagc tgacttacgg
    43381 attccagagt gcacaaaagc acaaggcgga agcagcgcat taatctgatt gttaggtagg
    43441 ttggtcaagt ccgtaatctc gaaagaggtt acggactttt tgtttatggg gtggaggagg
    43501 ttcagaccct ttttttaatg atgatggtaa gttgttgata attagtgctg cgggaaggta
    43561 aggataaaaa agggtgctgc aggagaatgg gatggttttg ctttatttac aacgggctaa
    43621 acgtgtagta tttgagttca ctgccgtaca ggcagcttag aaattcacag gtaacatact
    43681 ccacccgccc accatgttca ctgccgtaca gacagataaa atgcgaaaaa aaagctcgca
    43741 ctttcgtacg agctcttctt taaatatggc ggtgaggggg ggattcgaac ccccgatacg
    43801 ttgccgtata cacactttcc aggcgtgctc cttcagccac tcggacacct caccaaattg
    43861 ttttgctacc aaacctcatg ggtggcaacg gggcgctact atagggagtt ggagtaaaac
    43921 ggtcaagaag aattttaatg ataattattg tttgctcata ctgtaaacaa gttgtgcagt
    43981 atatctacat cgagacaagt tacggactta tacttccaaa gtacttcata catatcacaa
    44041 aataaaaagg ccggttaaac cgacctttta ctcgttcttt ctcttcgccc atcaggcggt
    44101 aaaacaatca gcgactacgg aagacaatgc ggcctttgct caggtcgtac ggggtcagtt
    44161 caacagtcac tttgtcgccc gtcaggatgc ggatgtagtt tttgcgcatt ttaccggaga
    44221 tgtgtgcagt aaccacgtga ccgttttcta actctacgcg gaacatggta ttaggcaacg
    44281 tttcaagaac ggtaccttgc atttcaatat tgtcttcttt ggccatctaa tcctctgggg
    44341 tatcactacc gtaatttgaa ccggcaagat aatgccgaag ttctgtaaat aagtaaagat
    44401 ttgcgcgcta aatcgcaaca aacaggttcg gcacattact ccgaaaacac acggctaagc
    44461 cgcaccaaaa gcgcaacgta taagggagcg gtgagataaa cgatgggcgt tacctgacgc
    44521 gaaaaattcc ttatcggcag cggggtaatg agcgtaacca actctgcgac cgcaattata
    44581 acactctggg gagaaatgtg ccgaaaacat tcattcttgt ggtgaaaaca agcatcgtgg
    44641 tacccagaaa ttattcggca atcgtccgag gcgcatttga ttgagataat taaggtaatc
    44701 ccggcgggga atttcgcagg caccaagcga tgctgtgtga tcgttaagga cctggcagtc
    44761 gataagctta ccgccatgac cgataaattc ctcacagaat accagaagcg ccgttttaga
    44821 cgcattttcc atccggctga acatggactc gccacaaaat agcgttccct gggccacgcc
    44881 gtacataccg ccgacaagct catcttcacg ccagacttca atggagtggg catggccgag
    44941 ttcgtgaagg cgatggtagg cttcgaccac gccacgcgtg atccaggttc cttcttcgcg
    45001 atcgctggca cagccttcaa tgacctgacc aaaagcgtaa ttcatcgtga cacgataggg
    45061 cgagcgttta tgaaatcgct tcatactacg gctgatatgc agtgattctg gccatagcac
    45121 cgcgcgggga tcgggcgacc accagaggat ggggtcgcct ggagaaaacc acggaaaaat
    45181 accacgctgg taagccatta acaggcgcgc agggctaaga tcgcccccaa gtgccagcag
    45241 gccgttaggc tcacgtaatg cgccttccgg ggaagggaag gctattgaat ggcgagaaag
    45301 ctgaaccagg cgcatgaccg caaaactcca cgcaagtcgg atcgttcaat aatagcttac
    45361 aaaccctgct tgaactggta ataacgcccc tgtctggcaa gcagctctgc gtgagtacct
    45421 tgctcaataa tttgcccgtt gtccatcact attatttgtt ggaaacgaga gagtccgcga
    45481 agtcgatggg tgaccattaa caccgttttc tcacgcatca tttctgcaag caattcaagg
    45541 atctggcttt cggttgtggc atctaagcct tcggtaggtt catccagcaa caccagtggc
    45601 gcatcatgta acagcgcacg ggcgatagcc agacggcgca gttcaccacc ggagagctgg
    45661 cgtccgcctt cacctaacca actgttgaga cctgcatcct cgagcagctt ctccagacca
    45721 acgcgacgca agctctccgc cagagcctca tcactactgc caggcgaggc gagtaacaga
    45781 ttatcacgca gcgtggcgct aaacagatgc actcgctgag gaacaacgct gatggtctgt
    45841 cgtagagccg cttcattcag gctggctatg gggctatcgt taagcaaaat ctcgccctgt
    45901 tgcgggtccc aagcgcgggt cagcagttgt aacagtgttg atttgccgca tccggttcgc
    45961 ccgagaatcg caatatgttc cccggcgttt acctgaagag aaatcccttt aagtgcctgt
    46021 tgggattgct ccggataagt gaactgaaca tcccgtaatg tcagcgaaac gcgatcggca
    46081 acacgagttt gggtatcagg gaaggtgact tccggttttt gatccgttaa atcagtgatc
    46141 cgaacggcag aggcaatgac ttgccccaga tgctgaaatg cacccgttac tggtgccagt
    46201 gcttcaaacg cggctaacgc gcagaagaca aacagggcaa ttaacgcgcc gggttgagca
    46261 ttgccgccaa cgccgccaga cgccatccac agcatcagga tcaccgctaa cgcgccaatg
    46321 agcagcatta tcgcttgcga caatgcggtc agttcagatt gacggcgttg cgcttccagc
    46381 cattgaattt ctgtattctc tagttgcgtg cgataacgat cgctggcacc aaaaatggtc
    46441 agctcagctt gcccttgcag ccaggccgtc agttgttggc gatactgtcc gcgaagatga
    46501 gtcagatttt gcccggtgct ttttcccgca cgataaaaca gcggtggcat caggaaaagc
    46561 gtcagtaaca taatgccgcc cagcgtaaag gcgagggtga aatcaaggaa acttaacccg
    46621 attgtcacca tcataatcac cacaaaagcg cccaccagcg gcgagataac gcgcaggtaa
    46681 agatgatcga gcgtatcaac atccgccacc acgcgattga gcaattcgcc ctgacgatag
    46741 cgcgccagtc cggcagggga gaggggcaac aatttgctga aggtgtaaat gcgcagatgc
    46801 tgcaacacgc ggaaagtcgc gtcgtgactt accagacgtt caaaataacg cccggcagta
    46861 cgggtgattg cggcaccacg cacgcccgca gcgggtagca tatagttgaa gctgtacagt
    46921 ccggcaaccc ccgcaaccgc tgaggccgag aggaaccagc cggaaagtgt caacagtccg
    46981 atactggcga gcagcgtcac aattgccagc acaataccaa gacttaacat ccatttatga
    47041 cgtttataca gtgctagata gggtagcaaa gcgcgcattt aaatctcctc ctgacgatgg
    47101 gccagtaatg tggcgaatgg gccaccagcc acacttaatt ccgcgtaacg tccttgctca
    47161 ataatccggc catcctgcat aacccaaatg acatcccagt cagcaagatc ttctaactgg
    47221 tgggtgacca ttaacgttgt ctggcgcaga gaggcggcat tcagcgcctc cattacgcgc
    47281 tgttcactgt gagcatcaag gctggcagcg ggttcatcca acagtaatag cgaacaggga
    47341 tttagtaacg cacgggccac cgccacgcgc tgcgcctgcc ccacggaaag gcgggcagcc
    47401 tggtcgccaa caggcgtatc aacgccttgt ggcaggagcg gtagaaactc gctgacccag
    47461 gcgttatcca gcgctgcttg taattcatgt tcgctggcat caggtcgcgc cagtagtacg
    47521 ttatcccgta atgttgctgc cggtaattgt ggattttgcc caacccagga gagatgttta
    47581 cgccatgatt ctggtgataa atcgcgtaat tctatcccgt tgattcgtag cgatccctga
    47641 tatgagagaa aaccagaaag cgcgttcaac agtgaacttt tacctgaacc gctgcgacca
    47701 accaacacgg cacgttggcc tgctggcaaa gtaaagttca gcggtccggc cagcgtttta
    47761 ccttccggcg acgtgataaa cagatcctcg gcctcaatgg tcaccggatc ggtcgatgct
    47821 aattccacct cgccacactg cggatgggcg agcggggttt ccataaacgt tttcagactg
    47881 tcagctgcac caacagcctg ggctttagca tgataaaacg taccgagatc gcgtaatggc
    47941 tggaaaaact ctggcgcaag gatcagggcc agaaaacccg cagccagcgt cacgccagta
    48001 tcgtagtgac caaaatccag ctcgccaaga taggaaaaac caaagtagac cgccaccaga
    48061 gcaattgaca gcgaggtaaa aaattcgaga atgccggagg ataaaaatgc cagccgtagc
    48121 acttccattg tccgttggcg gaaatcttcc gaagcagaac gaatactttc aatttcagct
    48181 tcaccacgac caaaaatacg caatgtttcc atgccgcgca ggcgatcgag gaaatgccca
    48241 cttaagcgag caagagcgag aaagttacgt cggttagcat cggcagcccc cattccaacc
    48301 agcgccataa acaacggaat taacggtgca gtgcccagca gaatgagcgc cgcagcccag
    48361 tttgagggga agattgccac cacaatcagc aacggcaccg acactgccag cgccatttgc
    48421 ggcaggtagc gtgcatagta atcatgcata tcgtcaattt gctcgagtac cagcgtcgcc
    48481 cagctccccg caggtttacc ctgaatccac gctggccctg cttgttgcag acggtcgaga
    48541 acctgccggc ggatggcaaa gcggatatgc tgcccggcgt gataacccac ccgttcgcgt
    48601 aaccagacca cccatgcgcg cagtacaaag gtcagaacca gtaacgtaaa gggaagcagc
    48661 agggcttcac ggggaatatt ctccataatc atatgttgca gaattcgcgc catgaaccag
    48721 gcctgggcaa tgatcaatat gccgctcaca aagcccagca gacgagaaat attcagccaa
    48781 cgttgggaga tgacgctttg ctgttttaac cagcgggtta actctttttg acgagattta
    48841 ttcattgcac gcttagcagg tgagttatca gaattatttg cagagcaatg ttacaacggg
    48901 gaaaaaataa aggcgaccca tagtcgcaag gtgtcgcctt ctttactttt gttactgatt
    48961 tgtaaaatta ttttgcgtca gctaaaccat cgaggtagcg ttccgcatca agtgctgcca
    49021 tgcagcctgt accggccgaa gtaatggcct gacgataaat atgatccatc acgtcacccg
    49081 cggcaaagac gccaggaatg ctggtctggg tggcattacc atgaataccc gactgtactt
    49141 tgatgtagcc gttttccagt tccagctgcc cttcgaaaat ggcagtattc gggctgtgac
    49201 cgatagcaac aaacagaccg gcaacgtcga gtgattcgat gttatcgctg ttttgcgtat
    49261 cgcgcagacg aacgccagtg acacccattt gatcgccggt cacttcttcc agcgtccggt
    49321 tggtgtgcag aatgatgttg ccgttctcca ctttatccat cagacgctta atgaggattt
    49381 tttccgcgcg gaaaccgtca cggcggtgaa tcagatgtac ttccgaagcg atgttagaca
    49441 gatacaacgc ctcttcaacc gcggtattgc cgccgccgat gaccgcaact ttctggttgc
    49501 gatagaagaa accgtcgcag gttgcacaag cagaaacccc acggccttta aaggcttctt
    49561 cagagggcag gccgagatag cgtgcagaag ctccggtggc aataatcagc gcgtcgcaag
    49621 tgtattcgcc gttatcgcca ttcagacgga acggacggtt ttgcagatcc accttgttga
    49681 tatgatcaaa aatgatttca gtttcaaatt tggtggcatg ttcgtgcatg cgctccatta
    49741 ataacggacc ggtcagatcg tttggatcgc caggccagtt ttccacttct gtggtggtgg
    49801 tcagttggcc gcctttttcc atgccggtaa tcagcacagg ttgcaggttg gcgcgcgccg
    49861 catagacagc agcggtgtat cccgccgggc ctgaacccag gataagcagt ttactgtgtt
    49921 tggtcgtgcc catgagatcc ccatagttgt tggcagacaa tgggcaggat tgtagggaat
    49981 ttactgacgt aaaaaaagag tatgacgatt ttgttaacaa tttgtgcaat cggcagcatc
    50041 gataagcagg tcaaattctc ccgtcattat cacctctgct acttaaattt cccgctttat
    50101 aagctgatta aatgatgaat aaacgcccct gttaatgaat atctggcatg ttgtactaaa
    50161 aatcgatgtt ttgctttgac aatcccctgg tgttttgcga aaacattcga ggaagaaaaa
    50221 aaacagtatt cttatatgcg cataaccatg catgtaaata ccatgtttac cgtgctagtg
    50281 aaatctacgt atggcgtgga cagacgccat tcgtgatgtc gatagctgcc acaaggcaac
    50341 ggtcttctca ccgtagaccc aggcattgcg cgccgtgaat cttcatgatt tcggtctatc
    50401 gtgacgggta gcgactctga acagtgatgt ttcagggtca gacaggagta gggaaggaat
    50461 acagagagac aataataatg gtagatagca agaagcgccc tggcaaagat ctcgaccgta
    50521 tcgatcgtaa cattcttaat gagttgcaaa aggatgggcg tatttctaac gtcgagcttt
    50581 ctaaacgtgt gggactttcc ccaacgccgt gccttgagcg tgtgcgtcgg ctggaaagac
    50641 aagggtttat tcagggctat acggcgctgc ttaaccccca ttatctggat gcatcacttc
    50701 tggtattcgt tgagattact ctgaatcgtg gcgcaccgga tgtgtttgaa caattcaata
    50761 ccgctgtaca aaaacttgaa gaaattcagg agtgtcattt agtatctggt gatttcgact
    50821 acctgttgaa aacacgcgtg ccggatatgt cagcttaccg taagttgctg ggggaaaccc
    50881 tgctgcgtct gcctggcgtc aatgacacac ggacatacgt tgttatggaa gaagtcaagc
    50941 agagtaatcg tctggttatt aagacgcgct aacacggaac aggtgcaaaa tcggcgtatt
    51001 ttgattacac tcctgttaat ccatacagca acagtactgg ggtaacctgg tactgttgtc
    51061 cgttttagca tcgggcagga aaagcctgta acctggagag cctttcttga gccaggaata
    51121 cactgaagac aaagaagtca cattgacaaa gttaagtagc ggccgtcgcc ttctggaagc
    51181 gttgctgatc cttattgtcc tgtttgccgt ctggttgatg gctgccttac taagctttaa
    51241 cccttcggac cccagctggt cgcaaacggc ctggcatgaa cctatccata atttaggtgg
    51301 gatgcccggt gcgtggctgg cagatacgct gttctttatt tttggcgtga tggcttacac
    51361 cattcccgtc attattgtcg gcggttgttg gtttgcctgg cgtcatcagt ccagtgacga
    51421 atacattgat tattttgccg tttcgttacg catcattggc gttttggcgc tcatccttac
    51481 ctcctgtggt ctggcggcaa tcaacgctga cgatatctgg tattttgcct ccggtggcgt
    51541 cattggcagc ttactaagca ctacgttaca accactgcta cacagtagcg ggggaactat
    51601 tgcgctgctc tgcgtttggg ctgcgggcct gacgctgttc accggttggt catgggtgac
    51661 cattgctgaa aaactcggcg gctggatttt aaacattctc accttcgcca gtaatcgtac
    51721 ccgtcgcgat gatacctggg tcgatgaaga tgaatatgaa gatgacgaag agtatgaaga
    51781 tgaaaaccac ggcaaacagc atgaatcacg ccgtgcccgt attcttcgcg gcgcgctagc
    51841 gcgtcgtaaa cggttggcgg aaaaattcat taatccgatg gggcggcaaa cagacgctgc
    51901 gttgttctcc ggtaagcgga tggatgatga agaagagatt atctacactg cacgcggtgt
    51961 ggctgccgac ccggacgacg tcctattttc gggcaatcgt gcaacgcagc cagaatatga
    52021 cgaatacgat ccattattaa acggtgcgcc aattaccgaa cctgttgctg tagcagctgc
    52081 tgctaccacg gcgacacaaa gctgggctgc gccggttgaa cctgtgaccc agacgccgcc
    52141 tgttacttct gttgatgttc caccttcgca acctacagta gcctggcagc ctgtaccggg
    52201 tccacaaacg ggagagccgg ttattgctcc tgcaccggaa ggttacccac agcagccaca
    52261 atatgcgcag cctgcagtgc aatataatga gccgctgcaa caacctgtac agccacagca
    52321 gccgtattat gcgcctgcag ctgaacaacc tgtgcaacaa ccttactacg ctcctgcggc
    52381 ggaacaacct gttcagcaac cgtattacgc aactgcgccg gaacagcctg cgcaacagcc
    52441 gtattatgcc cctgcgccag aacaaccggt ggcaggtaac gcctggcaag ccgaagagca
    52501 gcaatccact tttgctccac agtctacata ccagactgag caaacttatc agcagccagc
    52561 cgctcaggag ccgttgtacc aacagccgca acccgttgaa cagcagcctg ttgtggagcc
    52621 tgaacccgtt gtagaagaga caaaacccgc gcgtccgccg ctttactatt ttgaagaagt
    52681 ggaagagaag cgagcccgtg aacgtgaaca acttgcggcc tggtatcaac cgattccaga
    52741 accggttaaa gaaccagaac cgatcaaatc ttcgttgaaa gcgccttctg ctgcagcagt
    52801 acctccagta gaagccgctg ccgctgtttc tccgctggca tctggcgtga aaaaagcgac
    52861 actggcgacg ggggctgccg caaccgttgc cgcgccagtc ttcagtctgg caaatagcgg
    52921 tggaccgcgt ccgcaagtca aagagggaat tggcccgcag ttgccacgac cgaaacgtat
    52981 tcgcgtgcca actcgtcgtg aactggcgtc ttacggtatt aagctgccct cacagcgtgc
    53041 ggcggaagag aaagcccgtg aagcccggcg caatcagtat gattctggcg atcagtataa
    53101 cgatgatgaa atcgatgcga tgcagcagga tgaactggca cgtcagttcg cccagacaca
    53161 gcagcaacgc tatggcgaac agtatcaaca tgatgtgccc gtaaacgcag aagatgcaga
    53221 tgctgcggca gaggctgaac tggctcgtca gtttgcgcaa actcaacaac aacgttattc
    53281 cggcgaacaa ccggctgggg cgaatccgtt ctcgctggat gattttgaat tttcgccaat
    53341 gaaagcgttg ctggatgatg gtccacacga accgttgttt acgccaattg ttgaacctgt
    53401 acagcagccg caacaactgg ttgcaccgca gcagcagtat cagcagccac aacagcaggt
    53461 tgcgccgcag ccacaatatc agcagccgca acaaccggtt gcaccgcagc agcaggatac
    53521 cctgcttcat ccgctgttga tgcgtaatgg cgacagccgt ccgttgcata aaccgacgac
    53581 gccgctgcct tctctggatt tgctgacacc gccgccgagc gaagtggagc cggtagatac
    53641 ctttgcgctt gaacaaatgg ctcgcctggt ggaagcgcgt ctggctgatt tccgtattaa
    53701 agccgatgtc gtcaattact ctccggggcc ggttatcact cgctttgaac ttaacctggc
    53761 tcctggcgta aaagcggcgc gcatttctaa cttgtcacgg gaccttgccc gttcactttc
    53821 gacggtggcg gtgcgtgtcg ttgaagttat tcctggcaaa ccctatgtag gtctggagtt
    53881 accaaataaa aaacgacaaa ccgtttatct gcgcgaagtt ttggataacg ccaaattccg
    53941 cgataatccg tcgccattaa ccgtggtgct gggtaaagat atcgccggtg agccggtggt
    54001 tgccgatctg gcgaaaatgc cgcacttgtt ggttgcgggg actaccggtt ccggtaaatc
    54061 tgtcggtgtg aacgcgatga tcctgagcat gctttataaa gcacagccag aagatgtgcg
    54121 tttcatcatg atcgacccga aaatgctgga gctttcggtt tatgaaggca ttccgcatct
    54181 gttaacggaa gtcgttactg atatgaaaga tgccgccaac gcgctgcgct ggtgtgttaa
    54241 cgagatggag cgtcgttata aactgatgtc tgcgctgggt gtgcgtaatc tggcgggtta
    54301 taacgaaaaa attgctgaag ccgatcgcat gatgcgtccg attccagacc cgtactggaa
    54361 gccgggtgac agtatggatg cccagcatcc ggtgctgaaa aaagaaccat acattgtggt
    54421 gttggttgac gaatttgccg acctgatgat gacggtaggt aaaaaagtgg aagagctgat
    54481 agcacgtctg gcgcaaaaag cccgtgccgc gggtatccac ctcgtactgg caactcagcg
    54541 tccatcggtt gatgttatta ctggtctgat taaagcgaat attccgaccc gtatcgcctt
    54601 taccgtatcc agtaagattg actcacgtac cattcttgat caggctggcg cggaatcact
    54661 gctggggatg ggggatatgc tctactctgg gccgaactcc acgttgccgg tacgtgtcca
    54721 tggtgctttt gttcgcgatc aggaagttca tgccgtggtg caggactgga aagcgcgtgg
    54781 tcgcccacag tatgttgatg gcatcacttc cgacagtgaa agcgaaggtg gcgcgggcgg
    54841 tttcgatggc gctgaagaac tggatccgtt gttcgatcag gcggtgcagt ttgtcactga
    54901 aaaacgcaaa gcgtcaattt ctggcgtaca gcgtcagttc cgcattggtt ataaccgtgc
    54961 agcgcgtatt atcgaacaga tggaagcgca ggggattgtc agcgaacagg ggcacaacgg
    55021 taatcgtgaa gtgctggccc caccgccgtt tgactaatta atgcattgcc ggataaggcg
    55081 cggtagcgtc gcatccggca ctctatcaac tgaaaattca gtattttctt ttttcctcaa
    55141 gctgattatt agcctggaat agagagtaga gggaactccc gatcgggagt gacgtaattt
    55201 gaggaataat gatgaaaaaa attgccatca cctgtgcatt actctcaagc ttagtagcaa
    55261 gcagcgtttg ggctgatgcc gcaagcgatc tgaaaagccg cctggataaa gtcagcagct
    55321 tccacgccag cttcacacaa aaagtgactg acggtagcgg tgcggcggtg caggaaggtc
    55381 agggcgatct gtgggtgaaa cgtccaaact tattcaactg gcatatgaca caacctgatg
    55441 aaagcattct ggtttctgac ggtaaaacac tgtggttcta taacccgttc gttgagcaag
    55501 ctacggcaac ttggctgaaa gatgccaccg gtaatacgcc gtttatgctg attgcccgca
    55561 accagtccag cgactggcag cagtacaata tcaaacagaa tggcgatgac tttgtcctga
    55621 cgccgaaagc cagcaatggc aatctgaagc agttcaccat taacgtggga cgtgatggca
    55681 caatccatca gtttagcgcg gtggagcagg acgatcagcg cagcagttat caactgaaat
    55741 cccagcaaaa tggggctgtg gatgcagcga aatttacctt caccccgccg caaggcgtca
    55801 cggtagatga tcaacgtaag tagaggcacc tgagtgagca atctgtcgct cgatttttcg
    55861 gataatactt ttcaacctct ggccgcgcgt atgcggccag aaaatttagc acagtatatc
    55921 ggccagcaac atttgctggc tgcggggaag ccgttgccgc gcgctatcga agccgggcat
    55981 ctgcattcta tgatcctctg ggggccaccg ggtaccggca aaacaactct cgctgaagtg
    56041 attgcccgct atgcgaacgc tgatgtggaa cgtatttctg ccgtcacctc tggcgtgaaa
    56101 gagattcgcg aggcgatcga gcgcgcccgg caaaaccgca atgcaggtcg ccgcactatt
    56161 ctttttgttg acgaagttca ccgtttcaac aaaagccagc aggatgcatt tctgccacat
    56221 attgaagacg gcaccatcac ttttattggc gcaaccactg aaaacccgtc gtttgagctt
    56281 aattcggcac tgctttcccg tgcccgtgtc tatctgttga aatccctgag tacagaggat
    56341 attgagcaag tactaactca ggcgatggaa gacaaaaccc gtggctatgg tggtcaggat
    56401 attgttctgc cagatgaaac acgacgcgcc attgctgaac tggtgaatgg cgacgcgcgc
    56461 cgggcgttaa atacgctgga aatgatggcg gatatggccg aagtcgatga tagcggtaag
    56521 cgggtcctga agcctgaatt actgaccgaa atcgcaggtg aacgtagtgc ccgctttgat
    56581 aacaaaggtg atcgctttta cgatctcatt tccgcactgc ataaatcggt acgtggtagc
    56641 gcacccgatg cggcgctgta ctggtatgcg cgaattatta ctgccggtgg cgatccgtta
    56701 tatgttgcgc gtcgctgtct ggcgattgcg tctgaagatg tgggtaatgc tgatccacga
    56761 gcgatgcagg tggcaattgc agcctgggat tgctttactc gcgttggccc ggcggaaggt
    56821 gaacgcgcca ttgctcaggc gattgtttac ctggcctgcg cgccaaaaag caacgctgtc
    56881 tacactgcgt ttaaagctgc gctggcggat gctcgcgaac gtccggatta tgacgtgccg
    56941 gttcatttgc gtaatgcgcc gacgaaatta atgaaggaaa tgggctacgg gcaggaatat
    57001 cgttacgctc atgatgaagc aaacgcttat gctgccggtg aggtttactt cccgcgggaa
    57061 atagcacaaa cacgctatta tttcccgaca aacaggggcc ttgaaggcaa gattggcgaa
    57121 aagctcgcct ggctggctga acaggatcaa aatagcccca taaaacgcta ccgttaatgt
    57181 tatcgttgcg gtaatgttgt tactgtatcc ctgtggtcgc aggctgtggc cacatctccc
    57241 atttaattcg ataagcacag gataagcatg ctcgatccca atctgctgcg taatgagcca
    57301 gacgcagtcg ctgaaaaact ggcacgccgg ggctttaagc tggatgtaga taagctgggc
    57361 gctctcgaag agcgtcgtaa agtattgcag gtcaaaacgg aaaacctgca agcggagcgt
    57421 aactcccgat cgaaatccat tggccaggcg aaagcgcgcg gggaagatat cgagccttta
    57481 cgtctggaag tgaacaaact gggcgaagag ctggatgcag caaaagccga gctggatgct
    57541 ttacaggctg aaattcgcga tatcgcgctg accatcccta acctgcctgc agatgaagtg
    57601 ccggtaggta aagacgaaaa tgacaacgtt gaagtcagcc gctggggtac cccgcgtgag
    57661 tttgactttg aagttcgtga ccacgtgacg ctgggtgaaa tgcactctgg cctcgacttt
    57721 gcagctgcag ttaagctgac tggttcccgc tttgtggtaa tgaaagggca gattgctcgc
    57781 atgcaccgcg cactgtcgca gtttatgctg gatctgcata ccgaacagca tggctacagt
    57841 gagaactatg ttccgtacct ggttaaccag gacacgctgt acggtacggg tcagctgccg
    57901 aaatttgctg gcgatctgtt ccatactcgt ccgctggaag aagaagcaga caccagtaac
    57961 tatgcgctga tcccaacggc agaagttccg ctgactaacc tggtgcgcgg tgaaatcatc
    58021 gatgaagatg atctgccaat taagatgacc gcccacaccc catgcttccg ttctgaagcc
    58081 ggttcatatg gtcgtgacac ccgtggtctg atccgtatgc accagttcga caaagttgaa
    58141 atggtgcaga tcgtgcgccc ggaagactca atggcggcgc tggaagagat gactggtcat
    58201 gcagaaaaag tcctgcagct gctgggcctg ccgtaccgta aaatcatcct ttgtaccggc
    58261 gacatgggct ttggcgcttg caaaacctat gacctggaag tatggatccc ggcacagaac
    58321 acctaccgtg agatctcttc ctgctccaac gtttgggatt tccaggcacg tcgtatgcag
    58381 gcacgttgcc gcagcaagtc ggacaagaaa acccgtctgg ttcataccct gaacggttct
    58441 ggtctggctg ttggtcgtac gctggttgca gtaatggaaa actatcagca ggctgatggt
    58501 cgtattgaag taccagaagt tctgcgtccg tatatgaacg gactggaata tattggctaa
    58561 tacccaattt ttctgaatct aaaaagcgcc tgcgggcgct ttttttgtct ccctttgata
    58621 ccgaacaata attactcctc acttacacgt aatactactt tcgagtgaaa atctacctat
    58681 ctctttgatt ttcaaattat tctatgtata caagcctata tagcgagctg ctatagaaat
    58741 aattacacaa tacggtttgt tactggaatc aatcgtgagc aagcttgagt gagccattat
    58801 gaaaacgaaa atccctgatg cggtattggc tgctgaggtg agtcgccgtg gtttggtaaa
    58861 aacgacagcg atcggcggcc tggcaatggc cagcagcgca ttaacattac cttttagtcg
    58921 gattgcgcac gctgtcgata gcgccattcc aacaaaatca gacgaaaagg ttatctggag
    58981 cgcctgtaca gttaactgtg gtagtcgctg cccgctacgt atgcacgtcg tggacggtga
    59041 aatcaaatat gtcgaaacgg acaataccgg cgatgacaat tacgacggcc tgcactaggt
    59101 tcgcgcctgc ctgcgtgggc gttccatgcg tcgccgtgtc tacaatccgg atcgcctgaa
    59161 atatccgatg aaacgagtcg gggcgcgcgg tgaaggcaaa ttcgagcgca ttagctggga
    59221 agaagcctac gacatcatcg cgaccaatat gcagcgcctg atcaaagagt acggcaacga
    59281 gtctatctat ctgaactatg gcaccggtac gctgggcggc accatgaccc gctcctggcc
    59341 gccgggaaat accctggtcg cgcggctgat gaactgctgc ggcggctatc tgaaccatta
    59401 cggcgactac tcctccgcgc aaattgcgga aggtttgaac tatacctacg gcggctgggc
    59461 agatggcaac agcccgtcgg atatcgaaaa cagtaagctg gtagtgctgt ttggtaataa
    59521 ccctggcgaa acgcgaatga gtggcagtgg ggtgacttac tatcttgaac aggcacgcca
    59581 gaaatctaat gcccgcatga tcatcatcga tccgcgctat accgacaccg gtgccgggcg
    59641 cgaagatgag tggatcccta ttcgtccggg aacagatgcc gcactggtta acggtctggc
    59701 gtacgtcatg atcactgaaa acctggtgga tcaggcattc ctcgataaat attgcgttgg
    59761 ctacgatgag aaaaccctgc cagccagtgc gccgaaaaat ggccactata aagcttatat
    59821 tctgggtgaa gggccagatg gcgtggctaa aacaccggaa tgggcctcac aaatcaccgg
    59881 tgttccggca gacaaaatca tcaaactggc tcgtgaaatc ggcagtacca aaccggcgtt
    59941 tatcagccag ggatggggcc cgcagcgtca cgctaacggt gaaatcgcaa cccgtgctat
    60001 ctcgatgctg gcgattctga ccggtaacgt tggtattaac ggaggcaaca gcggcgcgcg
    60061 tgaaggttca tacagcttac cgtttgtccg tatgccgacc ttggaaaacc cgatccagac
    60121 cagcatttcg atgtttatgt ggaccgatgc cattgaacgt ggcccggaaa tgacggcgct
    60181 gcgtgatggt gtacgcggga aagataagct ggatgtgccg atcaaaatga tctggaacta
    60241 tgccggtaac tgcctgatta accagcattc tgaaatcaac cgtacccatg aaatccttca
    60301 ggatgataag aagtgcgagc tgattgtggt tatcgactgc cacatgacct catcggcgaa
    60361 atatgctgac atcctgctgc ctgactgcac cgcttccgaa cagatggact ttgcgctgga
    60421 tgcatcctgc gggaatatgt cttacgtgat tttcaacgat caggtgatta aaccgcgctt
    60481 tgaatgtaag accatctatg aaatgaccag cgaactggca aaacgtcttg gcgttgagca
    60541 acagtttact gaaggccgta cacaggaaga gtggatgcgg catctgtatg cccagtcgcg
    60601 ggaagcgatt cctgaactac caacgtttga agagttccgc aagcagggga tctttaaaaa
    60661 gcgcgaccca caagggcacc acgttgctta taaagctttc cgtgaagatc ctcaggcaaa
    60721 ttcactgacc acgccatcgg gcaaaattga gatttattcg caggcgctgg ctgacattgc
    60781 cgctacctgg gaattgccag aaggagatgt gatcgatcca ctgccgatct acacgccggg
    60841 ctttgaaagt tatcaggatc cgttgaacaa acagtatccg ctgcagctta cgggtttcca
    60901 ctataaatct cgcgttcact caacttacgg cgacgttgat gtgctgaaag cagcttgccg
    60961 tcaggaaatg tggatcaacc cgcttgatgc gcaaaaacgc ggtatcaaca acggcgataa
    61021 agtcaggatc tttaacgatc ttggtgaggt tcatattgag gcgaaagtga cgccacgaat
    61081 gatgccgggt gtggtcgcac tgggtgaagg tgcctggtat gacccggata caaaacgtgt
    61141 cgataagggc ggttgtatca acgtactgac cactcaacgt ccgtctcctc tcgctaaggg
    61201 gaatccgtca catacaaacc ttgttcaggt tgaaaaggtg taaggagtaa ccgatgacaa
    61261 cccagtatgg attttttatt gattccagcc gttgcaccgg ttgcaaaacc tgcgagctgg
    61321 cctgtaaaga ctacaaagat ttgacgccag aagtcagctt ccgccgcatt tatgaatatg
    61381 ctggcggcga ctggcaggaa gataacggtg tctggcacca gaacgtgttt gcctactatc
    61441 tgtcgatttc atgtaaccac tgcgaagatc cggcttgtac taaagtctgc ccgagcggtg
    61501 cgatgcataa acgtgaagat ggttttgttg tggtcgatga agatgtgtgc attggctgcc
    61561 gctactgcca tatggcttgc ccgtatggc
//
LOCUS       DAJTMC010000005        57490 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.005, whole genome shotgun sequence.
ACCESSION   DAJTMC010000005 DAJTMC010000000
VERSION     DAJTMC010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 57490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57490
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.005"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(<1..591)
                     /gene="wcaD"
                     /locus_tag="OO130_000295"
     CDS             complement(<1..591)
                     /gene="wcaD"
                     /locus_tag="OO130_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="essential for colanic acid biosynthesis; colanic
                     acid is an exopolysaccharide produced under stress
                     conditions that confers acid and heat tolerance; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HCR5363836.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGIGAGLTAFNYLFGQSLDASKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIK"
     gene            complement(566..1783)
                     /gene="wcaC"
                     /locus_tag="OO130_000296"
     CDS             complement(566..1783)
                     /gene="wcaC"
                     /locus_tag="OO130_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HCR5363837.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQAIFGTTLAGFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(1780..2252)
                     /gene="wcaB"
                     /locus_tag="OO130_000297"
                     /pseudo
     CDS             complement(1780..2252)
                     /gene="wcaB"
                     /locus_tag="OO130_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
     gene            complement(2255..3094)
                     /gene="wcaA"
                     /locus_tag="OO130_000298"
     CDS             complement(2255..3094)
                     /gene="wcaA"
                     /locus_tag="OO130_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000654502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HCR5363838.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGH"
     gene            complement(3272..5434)
                     /gene="wzc"
                     /locus_tag="OO130_000299"
                     /pseudo
     CDS             complement(3272..5434)
                     /gene="wzc"
                     /locus_tag="OO130_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
     gene            complement(5437..5880)
                     /gene="wzb"
                     /locus_tag="OO130_000300"
     CDS             complement(5437..5880)
                     /gene="wzb"
                     /locus_tag="OO130_000300"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HCR5363839.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSWETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(5886..6900)
                     /locus_tag="OO130_000301"
                     /pseudo
     CDS             complement(5886..6900)
                     /locus_tag="OO130_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
     gene            7209..7358
                     /locus_tag="OO130_000302"
     CDS             7209..7358
                     /locus_tag="OO130_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5363840.1"
                     /translation="MVIEYWLSNNAGGSVTANGIAGKVTIRQLAIFKNYIKCHSIWFL
                     GVSLG"
     gene            7559..9142
                     /locus_tag="OO130_000303"
     CDS             7559..9142
                     /locus_tag="OO130_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCR5363841.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEDEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLHKDGELEYEV"
     gene            complement(9416..11269)
                     /gene="asmA"
                     /locus_tag="OO130_000304"
     CDS             complement(9416..11269)
                     /gene="asmA"
                     /locus_tag="OO130_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HCR5363842.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRNYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEVVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIHGQGSFQAQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALSGAGTLNLAEQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(11291..11872)
                     /gene="dcd"
                     /locus_tag="OO130_000305"
     CDS             complement(11291..11872)
                     /gene="dcd"
                     /locus_tag="OO130_000305"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HCR5363843.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(11964..12605)
                     /gene="udk"
                     /locus_tag="OO130_000306"
     CDS             complement(11964..12605)
                     /gene="udk"
                     /locus_tag="OO130_000306"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002211872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HCR5363844.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHNLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            12923..16220
                     /locus_tag="OO130_000307"
                     /pseudo
     CDS             12923..16220
                     /locus_tag="OO130_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            complement(16259..17116)
                     /gene="alkA"
                     /locus_tag="OO130_000308"
     CDS             complement(16259..17116)
                     /gene="alkA"
                     /locus_tag="OO130_000308"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HCR5363845.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVSGVETVADDYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLFDLQCNPQIVNGALGKLGAAR
                     PGLRLPGCIDAFEQGVRAILGQLVSVAMAAKLTAKVVQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGSLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDGTDEL"
     gene            17250..18602
                     /gene="yegD"
                     /locus_tag="OO130_000309"
     CDS             17250..18602
                     /gene="yegD"
                     /locus_tag="OO130_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCR5363846.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPHLLKMENDSTLLPSMLCAPTRE
                     AVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQGILERAAKRAGFRDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEESKIALSNAAETRASLPFISDELATLISQQGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPLIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(18614..20536)
                     /gene="yegI"
                     /locus_tag="OO130_000310"
     CDS             complement(18614..20536)
                     /gene="yegI"
                     /locus_tag="OO130_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HCR5363847.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDIEEFVDSVAKIYHTP
                     PPASKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQSYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINVNGTLHLCEVGVSHFTPPELQTLSSFVGFERTENHDNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALETDITHFRYAYASDNQRRGLKPPPRSIPLSMLPSDVEAMFQQ
                     AFTESGVATGRPTAKAWVAALDSLRQQLKKCPVSAMHVYPAHLTDCPWCTLDNQGVIY
                     FVDLGEEVITTSGDFVLAKVWAMVMASVAPPALQLPLPDHFQPTGRPLPLGLLRREYI
                     ILIEIALSALSLLLCGLQAEPRYIILVPVLSAIWIIGSLTSKVYKAEIQQRREAFNRA
                     KMDYEHLVSQIQQLGGLEGFIAKRAMLEKMKDEILGLPEEEKRALAALYDTARERQKQ
                     KFLEGFFASIPGVGPARKAALRSFGIETAADVTRRGVKQVKGFGDHLTQAVIDWKASC
                     DRRFVFRPNEAVTPADRQAVMAAKRHRLESALTVGATELQRFRLYAPARTMPLMEPLR
                     QAAEKLAQAQADLSRC"
     gene            complement(20533..21294)
                     /gene="pphC"
                     /locus_tag="OO130_000311"
     CDS             complement(20533..21294)
                     /gene="pphC"
                     /locus_tag="OO130_000311"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase PphC"
                     /protein_id="HCR5363848.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVVWLNDQQPLLVMFL
                     ADGAGSVSQGGEGATLAVNEAMAFMVQKVQDGELGLNDVLATNMVLTIRQRLFAEAEA
                     KALAVRDFACTFLGLISSPDGTLIMQIGDGGVVVDLGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLETFTSTGRAHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASVTQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            complement(21291..21950)
                     /locus_tag="OO130_000312"
     CDS             complement(21291..21950)
                     /locus_tag="OO130_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCR5363849.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMSGRPINELNAGL
                     VTFRDELLADPLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFQGEEDKKFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            complement(22171..22230)
                     /gene="ibsA"
                     /locus_tag="OO130_000313"
     CDS             complement(22171..22230)
                     /gene="ibsA"
                     /locus_tag="OO130_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HCR5363850.1"
                     /translation="MMKHVIILVILLVISFQAY"
     gene            complement(22503..22559)
                     /locus_tag="OO130_000314"
     CDS             complement(22503..22559)
                     /locus_tag="OO130_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCR5363851.1"
                     /translation="MMKLLIIVVLLVISFPAY"
     gene            complement(22831..22887)
                     /locus_tag="OO130_000315"
     CDS             complement(22831..22887)
                     /locus_tag="OO130_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCR5363852.1"
                     /translation="MMKLLIIVVLLVISWPAY"
     gene            23166..24413
                     /gene="mdtA"
                     /locus_tag="OO130_000316"
     CDS             23166..24413
                     /gene="mdtA"
                     /locus_tag="OO130_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HCR5363853.1"
                     /translation="MKGSYKSRWVIVIVVVIAAIAAFWFWQGRNDSRSAAPGATKQAQ
                     QSPAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTIIAANTVTVRSRVDGQLMALH
                     FQEGQQVKAGDLLAEIDPSQFKVALAQTQGQLAKDKATLANARRDLARYQQLAKTNLV
                     SRQELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQIS
                     SGDTTGIVVITQTHPIDLVFTLPESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTL
                     LSLDNQIDATTGMIKVKARFNNQNDVLFPNQFVNARMLVDTEQNAVVIPTAALQMGNE
                     GHFVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGNRVVTDGIDRLTEGAKVEVVE
                     AQSATTPEEKATSREYAKKGARS"
     gene            24413..27535
                     /gene="mdtB"
                     /locus_tag="OO130_000317"
     CDS             24413..27535
                     /gene="mdtB"
                     /locus_tag="OO130_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HCR5363854.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLIFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFANMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDKGLVAYVNVDRDSASRLGISMADVDNA
                     LYSAFGQRLISTIYTQANQYRVVLEHNTEITPGLATLDTIRLTSSDGGVVPLSSIAKV
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            27536..30613
                     /gene="mdtC"
                     /locus_tag="OO130_000318"
     CDS             27536..30613
                     /gene="mdtC"
                     /locus_tag="OO130_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HCR5363855.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGKLYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRAPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRQPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            30614..32030
                     /locus_tag="OO130_000319"
                     /pseudo
     CDS             30614..32030
                     /locus_tag="OO130_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416581.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            32027..33430
                     /gene="baeS"
                     /locus_tag="OO130_000320"
     CDS             32027..33430
                     /gene="baeS"
                     /locus_tag="OO130_000320"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HCR5363856.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDEQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            33427..34149
                     /gene="baeR"
                     /locus_tag="OO130_000321"
     CDS             33427..34149
                     /gene="baeR"
                     /locus_tag="OO130_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HCR5363857.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            34340..34672
                     /locus_tag="OO130_000322"
     CDS             34340..34672
                     /locus_tag="OO130_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HCR5363858.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            34795..36195
                     /locus_tag="OO130_000323"
     CDS             34795..36195
                     /locus_tag="OO130_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000911981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HCR5363859.1"
                     /translation="MLKGLRTLFSRRVLFNVEMNNSVDCELLLIQQQLQALIAEPGNY
                     LAHDCDLNDTLVLCQLTHHHNGYIREKAVQYLGHKEDVAAIEELLLAANDWVKEVRLA
                     ATIALRHLMKDKNAAAFAVNLPMIRNLLQCQRYDHRAFVDEILRYLLTENNRPQLTYW
                     LTCPDKKLSRAVLQTLIEYGYFNDESSLLSILDKPDEGLRMLATTHWLRQQLPLSEAV
                     LTRLLKDRWPRIRQATLFSLTDRAIEMPPALHSTLLLDNNMLIRLRAKNMLHEVMDVP
                     QFWRHVVTSAEYTPSQRRAALYGLDSIHDPNILKLAEWGLSQNVFPLRLAAMHILAKA
                     NPRCGVKETILTTLANPDAAGLRFMVNICVWCRVPLTFEEIRQLQENAPSVKHACAYC
                     RLYHNLNKWDGLILLLQSQHKLTEEFAGKQLAIWQRNFNLSGIQPNALQRQQLQALFT
                     RNPELHNRLWGYIPFK"
     gene            36516..37877
                     /gene="yegQ"
                     /locus_tag="OO130_000324"
     CDS             36516..37877
                     /gene="yegQ"
                     /locus_tag="OO130_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HCR5363860.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREYFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGVAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(38207..38524)
                     /locus_tag="OO130_000325"
     CDS             complement(38207..38524)
                     /locus_tag="OO130_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5363861.1"
                     /translation="MKKIAAISLISIFIMSGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARSRISGAENIVKNDATHFCVTQGKKMQIVELKTEGVGLHGVARLTFK
                     CGE"
     gene            38938..39836
                     /gene="yegS"
                     /locus_tag="OO130_000326"
                     /pseudo
     CDS             38938..39836
                     /gene="yegS"
                     /locus_tag="OO130_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
     gene            complement(39918..40691)
                     /locus_tag="OO130_000327"
     CDS             complement(39918..40691)
                     /locus_tag="OO130_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR5363862.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSVSGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(40797..41837)
                     /gene="gatD"
                     /locus_tag="OO130_000328"
     CDS             complement(40797..41837)
                     /gene="gatD"
                     /locus_tag="OO130_000328"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCR5363863.1"
                     /translation="MKSVVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITIGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGSMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFTQAVRDIA
                     RNAMPGKVLLIP"
     gene            complement(41885..43240)
                     /locus_tag="OO130_000329"
     CDS             complement(41885..43240)
                     /locus_tag="OO130_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HCR5363864.1"
                     /translation="MFSEVMRYILDLGPTVMLPIVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNIDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSVSLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFITWRRARGFIKQEKAVLA
                     E"
     gene            complement(43244..43528)
                     /gene="gatB"
                     /locus_tag="OO130_000330"
     CDS             complement(43244..43528)
                     /gene="gatB"
                     /locus_tag="OO130_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HCR5363865.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQNHNIPVELIQCRVNEI
                     ETYMDGVHLICTTARVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            complement(43559..44011)
                     /gene="gatA"
                     /locus_tag="OO130_000331"
     CDS             complement(43559..44011)
                     /gene="gatA"
                     /locus_tag="OO130_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HCR5363866.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVEVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            complement(44021..45283)
                     /gene="gatZ"
                     /locus_tag="OO130_000332"
     CDS             complement(44021..45283)
                     /gene="gatZ"
                     /locus_tag="OO130_000332"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HCR5363867.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKEYVRAGFSKIHLDASMSCAGDPIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIVRGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQPLAQWIENTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLEGVDIPLGMISQYLPKQFERIQS
                     GELSAIPHQLIMDKIYDVLRAYRYGCAE"
     gene            complement(45312..46166)
                     /gene="gatY"
                     /locus_tag="OO130_000333"
     CDS             complement(45312..46166)
                     /gene="gatY"
                     /locus_tag="OO130_000333"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HCR5363868.1"
                     /translation="MHVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADAFYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPALDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQALKNYLTEHPEATEPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            complement(46474..47526)
                     /gene="fbaB"
                     /locus_tag="OO130_000334"
     CDS             complement(46474..47526)
                     /gene="fbaB"
                     /locus_tag="OO130_000334"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HCR5363869.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVVSTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            47783..49060
                     /locus_tag="OO130_000335"
     CDS             47783..49060
                     /locus_tag="OO130_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5363870.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGALLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQILGYADISPT
                     NIPLLITAGSSALLGVFAFFLPGTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFCESDNEITAIKVDDRDIALTQGEVK"
     gene            49057..50061
                     /locus_tag="OO130_000336"
     CDS             49057..50061
                     /locus_tag="OO130_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HCR5363871.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLERKGKIDPDLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPARDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSITRHAQQKRTTTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGINAIDPALKAELDAVNQLDFNRYATALAKYRQQREAV
                     "
     gene            50058..51023
                     /locus_tag="OO130_000337"
     CDS             50058..51023
                     /locus_tag="OO130_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCR5363872.1"
                     /translation="MSGARLHTLLPELTTRQSVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEELQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(50997..51743)
                     /locus_tag="OO130_000338"
     CDS             complement(50997..51743)
                     /locus_tag="OO130_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR5363873.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(51795..52613)
                     /locus_tag="OO130_000339"
     CDS             complement(51795..52613)
                     /locus_tag="OO130_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HCR5363874.1"
                     /translation="MQLRITSRKKLTSLLCALGLISIVAIYPRQKVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVAAPVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKSTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTVEELQAFVDGIKETP"
     gene            complement(52678..53478)
                     /gene="thiD"
                     /locus_tag="OO130_000340"
     CDS             complement(52678..53478)
                     /gene="thiD"
                     /locus_tag="OO130_000340"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HCR5363875.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIESDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQYV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            complement(53475..54263)
                     /gene="thiM"
                     /locus_tag="OO130_000341"
     CDS             complement(53475..54263)
                     /gene="thiM"
                     /locus_tag="OO130_000341"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HCR5363876.1"
                     /translation="MQVDLQSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQLRAQSMCAAVEQAKSSQTPWTLDPVA
                     VGALDYRRRFCLELLPHKPTAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEMDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     regulatory      complement(54298..54394)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(55975..56268)
                     /locus_tag="OO130_000342"
     CDS             complement(55975..56268)
                     /locus_tag="OO130_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5363877.1"
                     /translation="MYGQDTTAGGNLKKDSPLGKLWDFLHSPDLPTNPDFAKPADKTG
                     WVSSYSNPTYVPPTPTITAPQLKVDMAGNPLMTPPAWLNQPLNVNVNVKVQDG"
     gene            complement(56232..>57490)
                     /locus_tag="OO130_000343"
     CDS             complement(56232..>57490)
                     /locus_tag="OO130_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005135759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HCR5363878.1"
                     /translation="MIEGAGGAITIAATVALSGKIMTTGQDFESALSGLTAITGSTDK
                     AAKEFDYLRDQSNRLGLDLLKTSRDYTQFAAAVGDKLPKDQMRSIFEGASEWGLVTGA
                     SADEASRALKSLNQMMSKGTVMSEELKGQLSEALPGAVGLFVKALNQMKGTTNLTEKD
                     LFDLMQQGKLFSKDILPEVAKQMKAAARNGGALDKAMKSNRASWQRLQTSMQNAMNIF
                     FASGFGNELTSAFDAISAAVNGSGGAFEMFGHIAGKIVEGATDAFTELHDTVILTFRI
                     IEYYASKMGITGEQLKAWGEMAAYAAGVAMFAGSIWKLGVAFKWLLTMLNPLTKLLGV
                     MKGIAAIGGIEAATTGGSGGEGKDKPNKPGRGPKVGKVGGAAALSGGPVGWSIAVGSV
                     GGDYAWDKVMTLTTPCMDRTPPPGAI"
BASE COUNT        13671 a        15096 c        14534 g        14189 t
ORIGIN      
        1 tttgatacca aactgtttga tgctgagcca aattgagatt aatgccagag cgaaaaatgc
       61 cggttcgaaa taaagtgctg tcgtgcgctt gccgccgaat ttaatgaaat tcagcacata
      121 gctgttgctg taaatcagat atttcgaaat cgactccata atactgctgc caccggtgag
      181 gataatttgc gccatctcca ctgccgccag cgccaccacc agccctacca ccagataaaa
      241 gaaacgtaat atcttgcgat ggttgtgtgg cgaaattgtt ttaaaacgaa tactccacac
      301 catgccaata atgatcacaa tatagacaaa cagcatggtt gaagtgacgt atttactggc
      361 atccagcgac tgaccaaaca ggtagttaaa cgccgtgagt cccgcaccaa tccctaaggc
      421 aatcatcaat tttttaacgc tgatgcgttc taaaaacagc agtaacaaga caggtaaaaa
      481 agtgacgata gtgatgggga aactttcacc aagctgggcg attttgacgt taaccagcag
      541 gtagatcagc ggcagcagca ggtagctaca gattctgata gaagttgaca tactcctcca
      601 gcatctgttg tccactgtag gcggcgcggc tgcgctggct gaacccggcc aacgtggtac
      661 caaatatcgc ctgcgcgatt tccggtttgc ttaattgcac tagttgcagc acctcttctt
      721 cgctgacggt tttaccgccg gatttttgca acacttcccg cgccgcgtcg ctatgggtgg
      781 caatcaccgg cacgccaatc gatagcgcct cacacaaaat cagcgggtag ttatcgacgc
      841 gagaactgaa caccagcgca tccatctgat tgagcgcgct catcaacttg cgcttgtcag
      901 tttcaaagcc gtgattaacc acgttgccag cggtgaacgg cgagaactta ccaaaggtat
      961 gcagttcgat tttgtcgccc agcgccatca tctcacgcac cagttgctgg ttagtcttgc
     1021 cgtcgtaacg caagtcatgc gccaccaccg cgattttcgg cttgccctgg gtttcgcgca
     1081 ccggaggcaa gtccgccaga atcgcttcgg ttgccatatc aataccatta ttgataatcc
     1141 ggcaacgccc tggaccgtac aggctattga aagcgtcagc cacatgctgg ctgggggaaa
     1201 taaactgaca gcccagcgcc agcatctcac ggaataactg gcgtttgccc gccaccagct
     1261 ggtgtgcgcg atcaatcttc accggcggat agttattcaa ggtcgggcat ttctggcagc
     1321 ccgttttcca gccttcgcaa ccgtcggtaa aggcgcagcg tccggtaaca ctccagtggt
     1381 cgtgcagcgt ccagaccaga gtgacgtccg gtttatggtt tttcactttt tcgcaaaagc
     1441 gcaccacgct cttaagattt agccagtagc tgtgcagcac atgaaaatgc aggaccaccg
     1501 gacccggtgt gcgagtaatg gtgcgatata actcattgaa attgccaaac agatcgcgat
     1561 taaacagacg aaacagggca atattcgcca tcgcggtcat ccgcggcgta tgtttgatga
     1621 cctgcggata gttctgatgg ctgacgctct ctttgccgcc tttgccgtaa ccgtagacaa
     1681 aatgtgacgc cagcccctgt tgcagcgcac gctggtggag atctaacgcc acacctgctg
     1741 ccccgccttc cgccagtcgc acattaaatt gcagaatatt catttaatta ccttcactcg
     1801 cgctttttct cctaccacca gcgcgttgtc cgggacagag tcgagcacca cgctgcccgc
     1861 acccacggtg acgttgttac caagcgtgat atcaccaaga ataatgacgt tggcaccgag
     1921 ttcgacgccg ttgccaatgt gtggacatgc catgttatcg gcaccacgat tgccgatagt
     1981 gacgccgtgg cgaatggtga aatcatcccc cgctaccacg tttttattga tcactacggc
     2041 gtaaccgtga tggatagtaa agcggcggcc tggatttcat aaccgaaaaa gcattcggtg
     2101 ataatgcgat acagcaccag cagcggggcc gcccacagat tgttgaggac gttctttttg
     2161 cgccacaccg aacaaaaatg agcaacacga taggcaagaa ccatgcagca cgggcgtaaa
     2221 ctccagctgt tggcacgcag atcttccagc atctttagtg cccccgaata ccatcagcca
     2281 gacgtttgcc gttacgcacc gacagcagcg tcagcaaagt gcgccaggtc atacgtttat
     2341 tgcggatctg gtagagggta aacagctgat attttttgct tgcacggtca aatttatctt
     2401 tgtgctttcg gtaaaagtgg aagtacccgg agaatttttt cggtgacgag gtgatctgca
     2461 tttcaccgtg attgatatgc aggatctgcg tcgcttcttc cactttccac ggttcgccgt
     2521 actccaccac catccgcagg aagatgtcgt aatcctgtgc tgctttcagt tcggtatcga
     2581 acaaacactc tttgaaacgc catgcccaag taaagacctg attgccaatg atattgcgtt
     2641 tatagaacag gcggcgtgaa tacggcgatt tgggatatag cggcaaactt gctggttggg
     2701 aatagacttc gccctggcaa acgtagtcgt tagcgtacaa aaaggcgtga gtgaccagtt
     2761 gctgtttatg ggcgaggaag acgctcagac ggttgggtgt ccattcgtca tcgtcatcaa
     2821 tcccggtgat gtattcccct tgcgccagca taatcgcctg gttacgtacc gcgcacgccc
     2881 cgctgttaat atcgttgtgg atgtaagtga tacgcggatc gttgagggcg gtgacgtact
     2941 gttgcagttg ttcccaggag gtggagcaat catccacgat gatcatctcc cagttgctgt
     3001 agtcctggcg caggaccgat tttatcgccc gaatcgccag ttgttggcgg ttccaggtcg
     3061 gcatatagat tgagatcagc ggattgtctt tcataggttg ttctcccgga gtgtcgagat
     3121 gttctgccgg tttataagct tgtttacggg ttctgttgcc ggatgcggcg tgagcacctt
     3181 atccggccta cggagctggt ttgcttgccg gatgcggcgt gaaggtaggt cggataagat
     3241 gcgtcagcat cgcatccgac agcggatgtc gttatttcgc atccgactta tattcgtatt
     3301 cgtaatagcc ataatcctga tacgcgctgg cgcggcggaa gatggagttc agaatcaccc
     3361 ctttcaccgg aataccgttt tgctcaaagc ggctcagact ggtttccact tctttcaagg
     3421 tgttgaccgc ataacgcgcc accattaacg tggttccgac atgacgacca acaattgccg
     3481 catcggtcac tgccagaatc ggcggcgtat caatcaacac caggtcatag tttttactcg
     3541 cccagttcac cagttcggca aagcgttcgc tcatcaacag ttcagaagga tttggcggca
     3601 cctgaccgcg cgggatcagg tcaaatttgg caatagaggt cggtttagca gcggtagtaa
     3661 tatcgccctg accaatcaga atttccgaca ggccattaac gttattagtg cacagcagct
     3721 cgtgggtgta gcctttgcgc atatcgcagt cgatcaacaa cacccgttta ttggtctggc
     3781 tgatcaccgc cgccaggttg gcgcagacaa aggttttacc gattgacggg ctaaccccgg
     3841 tcatcatcaa cacattgttc tgtgcctgca tcatcgcgaa gtgcaaactg gtacgcagac
     3901 tgcggatggc ttcaatcgcc agatcggttg gattccccat cgccagtagc tggctctgtt
     3961 tatagcgttt aatccctttg atggttttga cgctatcgcg cgctttctgc cattccgaca
     4021 gcgggatgct ggcatagacg ctgataccgt gttcttccag cacctgcggg ctttcgatgc
     4081 cgcgattaaa caacgagcgc agcagcacac ccacgataga aagcatcagg ccaaggataa
     4141 tcgcgccgag gataatcagc cctttcttcg gttttagcac gccaggctgg gtgattgccg
     4201 ggtcaacaat gcgcacatcg ccgacggtgc tggcttcggt gattttcagc tcctgctctt
     4261 tattcagcag ttgcatatag acctgctgac cagactcgac atcgcgggtc aggcgcacaa
     4321 tctcctgctg ggttttcggc atcgccgtta cgcgaccgtt aagtttggct ttctcttctt
     4381 ccagcgcctg acgtttctcc agcagcgtgc ggtacgccgg gtgaactttg gtgtacagct
     4441 tggagatttc cgcctcttta aaggtcagtt cgttcaactg cgcgtcgatg ttcaccatcg
     4501 aatcgagcac cgctttcgct tccagcggca gatcaacaga atctttatcc tgacggaagg
     4561 cattcagttt gttttcggca acatcgaggc ggctacgtac ttccggtaac tgttgcgcga
     4621 ggaaagcgag gcttttcgac gcttccgccg atttgcgctc aatattttgt tcctgatagt
     4681 tacgggcgat gctgttaaga atgtcgcgga tctgttcgcg atcttcaccg gtataagtca
     4741 ggctcagtac gcctgcgtct ttgccgttct ccgttaccat caggctgttt tgcagttggt
     4801 tgatcatccc caacgtggag tatttggtga cggtaaactc actgcccggg cgggcgtgaa
     4861 tggcttcaac catcagcgtg acgccttctt ttttcagcat ctggcccgct tgcccacggg
     4921 cgctaaagcc gccatcactg ctcagggtgt agtttttatt gtccagcaca ttaagcgtaa
     4981 acacctagtc cgccatctct ttcgggcggt taaaggtagt cactttcacc gtctcgttct
     5041 ggcgtcccat cagtcgatcc cagcccgccc cgaaaatcgg gaacgtgttt ttgctcactg
     5101 caatatcgag gtcgagatcg tccaccgttt taccaagcac cagacgcgaa cgaatcaact
     5161 ggatctcggc gtccgatgca ggcggtttgt tggctaatgc cgaaccaatg tcctgcacta
     5221 acgaattgcc gctgttttgc tcgatttgta ccagtgcgtc ggcactataa atcggcgtgg
     5281 cgaagaaggt gtaaaccacg gcacagagga caaatacagc ggtgatgcca atcacccacc
     5341 agcgcgcttc aatgacggtg ccgaccaggc gaccaatatc gatttcatca ctgcccgtta
     5401 ccggagcggc atgttgtttt actttttctg tcattcttat acctgctctg cgttcaatgc
     5461 ctgcgcccac tggcgggcag accgttcaag taatgtgtac accgctgcaa acgtttccca
     5521 gcttttgcga tacggatcgg ggatttcaca ttcgttatcc cagtgaccaa acagcatcac
     5581 tttgccgcgc atctcaggtg ccatctcgca taagcgttcg atatggcgct tttccatggt
     5641 caaaatcagg tcatagttgc gacacaggcg gcggctgatt tgtcgtgcac agtggccttc
     5701 cagagacagt tgatgttctg cggcgacgct gatagcggta ggatcagcgc ccttaccgac
     5761 cagcgcgccg agtccagcgg actccacttt cagttccggg tgataacgtt gcagtaagcg
     5821 ttccgccgtc ggggaacggc aaatattgcc gacacagaca actaatatgt tgttaaacat
     5881 gacgattacc agttatgaat gtcgctggct gtatccgtca tgtaacggac accgctaata
     5941 gttggcaatc ttgccgttac gcccgccctc gcctttcagc ggacgaatga caaagatgcc
     6001 gctggcgttg gaggtggtca tgtcgatacc ttcagcatta cccagggctt cagtcagggc
     6061 ttcagtcagg gtcatgccgc taaagtccat tttcagggtg ctctgtttct tcacttcacc
     6121 catcacaaat actttcagat catcattacg cggcacgtag agaatatcgc cgggataaag
     6181 cagtcggttc tgattgaggt cgccgttttg catcagcacc tgcaaagaaa tgcgttcttc
     6241 acgaccattg tgtgttagca ccacgttgcg ccagtcagcg gtgtcggtca ggccacctgc
     6301 ggcgttgatg gcgtcgagaa tggtcagtgg tacgttggtg atcgcctgtt gaccggattt
     6361 attcacctga cctgagatat aggtcttttg tgagcggaag gcggcgatat taacgtccac
     6421 ctgcgggtca gcgatgtacg tcgctaagcg cccggtaata tcactgcgga tttcagcgag
     6481 cgttttcccg actacgtgaa ccttgccgat atacgggtaa aacatagtgc cgtcaggctg
     6541 tacccagttg ccggtgtcgc tggagctgcg gtactgacca gctggcgtgg tgagttccgg
     6601 gtgatcccag acggtgacat taagaacgtc ccccggcccg acgcgatact ggtaattcgc
     6661 gatctcgctt tccagcgtca tattggggcg cgctacattc gggcgtgggc gtaattggtc
     6721 aatcaggcgc ggggtcagcg gataaacatt caccattttg tcgagatcga aatcagcgtc
     6781 ctgctgtttg atgacgtctt tgcccatcgt cgacatattg ctgcccggaa gtactgtgca
     6841 accgcttatc aaggttactg acaccaataa tggcatcaat ttcattttgg atttcatcat
     6901 tgtttattta tcactttggc agagtaatta tcctgtgcac tattaatagc aatgtcgcca
     6961 tgcacattta ccttgcagtt aattgaatac aaatttaact ggcatcagtc ctaaaaaaat
     7021 tgatttcatc cgcaggctat tgacagaata attcagactg gtctttcagg catccagaca
     7081 cgctaccgcc cctggctttt tagccaccaa tacactgatt tagcttaatt ttttaaaccc
     7141 tctctgcata caatcgttga tgagaaaggg ttattgcgga aattaacttc cgaatataag
     7201 gtgacattat ggtaattgaa tattggctct ccaataatgc aggaggaagt gttacagcta
     7261 acggaatagc aggcaaggta acaattcggc aattggctat ttttaagaat tatattaagt
     7321 gtcattcaat atggttttta ggagtttctt taggttgaca atatttaata ttgcgcctcc
     7381 gcatgcgata tttcttaaat aatgttttat tattaccact tttaattcag ggataatgtg
     7441 aggttattac cctcaaataa tatgagataa atagtgctgc aacattgcat ttttgccccg
     7501 atttatccat gatcgaattg tgacatttgt catacaacga ataggttttg tacttactat
     7561 ggaatggatt gccgatccgt ctatctgggc cgggttaatc acgctgattg tgatcgaact
     7621 ggtcctcggc attgataacc tggtctttat tgccatcctc gccgaaaaac taccgccgaa
     7681 gcagcgcgac cgcgcacggg ttaccgggct gttgctggcg atgttaatgc gcctgttact
     7741 gctggcgtca atctcctggc tggtcaccct gacgcaaccg ctgttcagct tccgctcgtt
     7801 tacctttagc gcccgcgact taatcatgct gtttggtggt ttcttcctgt tgttcaaagc
     7861 cacgatggag ctgaacgaac ggctggaagg gaaagacagc aataatccca cacaacgcaa
     7921 aggtgcgaag ttctgggggg tggtgacaca aattgtggta ctggacgcca tcttctcact
     7981 cgactcagtg attaccgccg tcgggatggt cgaccattta ctggtcatga tggccgccgt
     8041 ggttatcgct atcagcctga tgttgatggc cagcaagccg ttaacgcaat ttgttaacag
     8101 tcacccgacc atcgttattc tctgcttaag cttcctgttg atgattggct ttagcctggt
     8161 ggcagaaggt ttcggcttcg tcattccgaa aggctacctg tacgctgcca ttggtttctc
     8221 ggtaatgatc gaggcgctca atcagttggc tatctttaac cggcgacgtt ttctttccgc
     8281 taaccagacg ctacgccagc ggactaccga agcggtaatg cgcctgctta gcgggcaaaa
     8341 agaagatgcg gaactggacg ccgaaaccgc gtccatgctg gtggatcatg gtaaccagca
     8401 gatctttaat ccgcaggaac ggcggatgat tgagcgggta cttaatctta accagcgtac
     8461 tgtcagcagc attatgacgt cgcgccacga tattgagcat atcgatctca acgcgccgga
     8521 agacgagatc cgccagttgc tggagcgaaa tcagcatacg cggctggtgg ttaccgacgg
     8581 tgatgacgca gaagatttgc tcggtgttgt tcacgttatc gacctgctac aacagtcact
     8641 gcgtggcgaa ccgctcaacc tgcgggtgtt gattcgccag ccgctagtgt tcccggaaac
     8701 cttgccgttg ttacctgccc tggagcagtt ccgtaatgcc cgcacacact ttgcttttgt
     8761 ggtggatgag tttggctcgg tggaagggat tgtgacatta agtgacgtca ctgaaaccat
     8821 tgccggtaac ttaccgaacg aagtggaaga gatcgacgcc cgccatgata ttcagaagaa
     8881 tgcggacggt tcctggacgg cgaatggtca tatgccgctg gaagatctgg tgcaatatgt
     8941 gccgctgccg ctggatgaaa aacgtgaata tcacaccatt gccgggctgt tgatggaata
     9001 tttacagcgt attccaaaac ctggcgaaga agttcaggtg ggggattatt tgcttaaaac
     9061 gttgcaggtg gaaagccatc gcgtgcagaa ggtgcagatt attccgctgc ataaggatgg
     9121 cgagttggag tacgaggtgt gatggtatcg gtcattttac ccaatagccg aaaggaataa
     9181 gatgagggaa ccgcgccagc ggcgatttac cgccgggagc ccgggttgcc agggtggtgg
     9241 cgattgagcc cccctggcac gttcaccggc ttgagcgttc catagtagcg aggaacatca
     9301 agtgaacgga acagccttcg atcttacata ttcagaatat aatgatgccg ccacgaacgc
     9361 ctgatgcgac gctttacgcg tcttatcagg cctacaggac aggtcaccag gtaacctaca
     9421 tcttctccag caacttcttc acatctttgc cattgcggga atctttattc cgctcggccc
     9481 agtcattcag acgacgtttc gcttcgtcct gtagatgttt gcgcagtaac tgatccactt
     9541 gcagactgta attgagttgc tgccagttgc cataaacccg cagcggtact ggcgtttctt
     9601 taaggaaatc aatcagtttg ctttccccgt tccagccacc cacgacccga atatcaaact
     9661 gggtgtcgca ggtttgctcc gccagattca acgtaccagc cccggaaagc gccagcacag
     9721 gcgattgacc ttgcatgtcg tttaacgtca cgacgccatc cttcaacgtc aaatcggtgg
     9781 taaagcggtc aagacgcgtt acgttatcga agttttcagc ggccttcaca tcaccaccat
     9841 tacgttctac cgcttgctga atcatctgct ggaagttcat cccttccatg cgcgtgtcgg
     9901 tcatttcgac atgtgcctgt ccttgccagt tgtggcggaa tgcgtcggca tctatgtcag
     9961 caccggagaa atcaccagcc agtgacatct ttccggtcaa cgaaatcgga tagttaaacg
    10021 ccttcagaat ggtgccaatc tcaacgtttt ccagccgtgg ctggaagttt atccgcggat
    10081 ttattgatgt cgcgtccagc gtgcccggca gtgaaacctg tccaccgtta agcttgccct
    10141 gcagttgagt aatttccagc aaacccgact tgttggtcat ttgcgtggca acatctgtaa
    10201 aattcattcc gcgccagcgc acgttactgg cctgcaacaa aatatcagcc gtaaagcctt
    10261 gcagtccctg atatgccggt tcatcaatac gcgaagaaat gaccgggcgc ggcaacgttg
    10321 attggctctg cccctgctgc gcggcaccgt tttcaccatt cgctgtttca ttaagcggga
    10381 tgaggttgtc aagattcagt tgcgggaatt gcagtctcag ctgccattcc ggtttctctg
    10441 tcagcgtgac ctgtgcttgc ccgctcagcg tactgtcatt ggcggtcaaa ctaatttggt
    10501 taaatgaaag gcgtttatgt gactcctgcc actgggcctg gaaactcccc tgcccatgaa
    10561 taccttgttt tggtaaatct gcaccctgca actgccagtt aatttgttca atagccgccg
    10621 ttaaatcatg cggataatca gaagcatcta ccgtaccgtt aagggatatc gtgagatcgc
    10681 gctgatcgcg attaacccgc ccggagaact caaatgaacc acgatgttgg ggatcttgtt
    10741 ccatttgcag gcgaatattg cggattgtca cctgctcgtc atcttcatgc tggaacacca
    10801 gcacgctgtc cgccacctta agactggata tatcaaacga ccatccgcga tcgtctgaca
    10861 gatccggcaa ggtattgtcg cgcggtgcaa ccggagcgtc ttcactgcgc accacttccg
    10921 tctgcggcgt cagttggatc actgcccctt ttagcatcac ctgcttaacg ctcagttgat
    10981 gactcagtag tggtaaaagc gccacgtcca gacgcatgtt gtcggcgcga accagtggct
    11041 ggctcgcgcc ctgggcggtg agagacattc gcccagagag gatactaagc tgcggccaga
    11101 cgtgccaacg cagtggcccg tcgagctgca attgataacc gctacgtgca gcaacttgct
    11161 tgaccatata gttgcggaaa tcattcggat tcaccagcaa cactaacgca gataacccgg
    11221 ccaccagcac gaccaggagt atcatcagcg tcgtcagaaa tcgtctcatg gtatcctcaa
    11281 tgggcctgaa ttagtcttta tcgattcggc tggctaccgc gccctgctgg ttgcgatatt
    11341 tcgcatcttc acggcggttg taaggtcgcg ccgccgggcc ggaaagcggc tcaaagctca
    11401 gcgcaccaat taacatgccc ggacgcagcg ccagcggcag cttaccggag ttgtagaact
    11461 ccagcacaat gcaaccagac cagcccggat cgatacggtg cgcggtgacg tgcaccatca
    11521 gccccagacg cgccaaagag gaacgcccgt ccagccagcc caccagatcg gctggcagcg
    11581 tcaccgactc cagcgtcacc gccagcgcca gttctcctgg gtgaagatag aacgcctcgc
    11641 cctcgtcgag aacgatctca tcgctcatca cgcggtcaag cgcggcgctc acttcatctt
    11701 tgggaccgct cagatcgata aacgctgccg tgtgaccacg gaaggtacga aatttattgc
    11761 ccaggcgtac atccaccgtc gcgccgttaa tacgctccac tggcggacgt gggttgatcg
    11821 acaaacggcc ttcatcaagc caggcttcaa tatctcggtc acacagacgc atggcatttt
    11881 ctcctttcgc gcatcactcc cttaacgcca attacgtcaa gggcatacca ggttatcact
    11941 gaacggtaca caatttatca agcttattca aagaactgac ttattttcgc tttcaatata
    12001 tcgatcgcga tgcggttttt cccgccgcgc ggcacgataa tgtccgcata ttgtttagaa
    12061 ggctcaatga attgcaggaa catcgggcgc acggtttttt gatattgcgc catcacggaa
    12121 tccattgaac gcccacgctc gttaacgtca cgcttgatgc ggcgcatcag gcagatatcc
    12181 agcggggtat caacgaaaat ggagaagtta agttcgtcac gcaaacgcgc atccgtcagc
    12241 aacaaaatgc cttcgagaat gatgaccttc ttcggctcaa ccgtcaccgt ttctttcata
    12301 cgcgtatgtt caacatagct gtaaaccggc aggtcaattg ccgagccgcg tttcaacgct
    12361 tgtaaatgct caagcagcag gttgtgatcc atcgcgctgg gatggtcgta gttggtctta
    12421 acgcgttctt ccatcgacag atggctttga tctttgtaat agcagtcttc gggaattacg
    12481 ccgatgtgtt catcaccgac ttgctcacgt aattcacgat aaagggtact ggcaataaga
    12541 ctcttgccgg aagccgatgc gccagcgata ccgataatga cgcactgatg agactgatca
    12601 gtcatatatt tagcgacctg attaacctgg atgttaggaa gggggcgacg aagcgccaaa
    12661 cgcggcaatt atagggattt catccgcctg ataccagtcg aatagcgttg ccgcgcgctc
    12721 agagttaatt gttgacaaag aattcccggt ggcaaattac gttgatcagt tttatgtaag
    12781 gtaaaaaatg ttatacgcag ttgcgcaaat tatccgcctt tacgtcactt tatgagcaat
    12841 tcacatataa aatgtaaaac ttttgtacta gcataaacac agaaacgaat actggcgacc
    12901 tggtcttgcg gataaagcgg taatgagcaa acaatcacag catgtattaa ttgccctgcc
    12961 ccacccactg cttcacctgg tcagtttagg tttagtctcg tttatcttta cccttttctc
    13021 gcttgagctt tcgcagtttg gcacccaact cgccccactg tggttcccga cgtccatcat
    13081 gatggtggcg ttttatcgcc atgccgggcg catgtggccg ggaattgcgc tgagctgctc
    13141 gctgggaaat atcgccgcat ccatcctgct tttttccacc agctcgctga acatgacctg
    13201 gacgaccatc aatattgttg aagccgtggt cggggcagtg ctgctacgta aattgctgcc
    13261 gtggtataac cccttgcaaa atctggctga ctggctgcgt ctggcattcg gcagcgccat
    13321 tgttccgcct ctgttagggg gtgttctggt tgtcctgctg acgcccggag acgatcctct
    13381 cagggcattt ttgatatggg tactgtcaga atccatcggc gctctggtac tggtgccgct
    13441 gggattgtta tttaagccac actatctgct gcgccatcgc aacccacggt tactttttga
    13501 gtcactgctc acattagcca tcacgctgac gttaagctgg ctttctatgt tgtacctgcc
    13561 gtggcctttt actttcatta ttgtgctgtt gatgtggagc gccgtgcgcc tgccacgaat
    13621 ggaagccttt ttgatcttcc ttaccacggt gatgatggtg tcgctgatga tggccgcgga
    13681 tccctccctg cttgctacgc cgcgtacgta cctgatgagc catatgccgt ggctactgtt
    13741 tttgctgatc ctgctgcccg ccaacatcat gaccatggtg atgtatgcct ttcgtgcgga
    13801 acgcaaacac atttccgaaa gcgaaacccg ctttcggaac gcgatggaat attccgctat
    13861 cggtatggcg ttagtgggca ccgagggaca atggctgcaa accaacaaag cgctctgcca
    13921 gtttctcggg tacagtcagg aagagctgcg cggactcacc tttcagcaac tgacctggcc
    13981 ggaggatctc aataaagatc tccaacaggt tgaaaagctg ataagcggtg aaataaacac
    14041 ctattcaatg gaaaaacgct actacaaccg taatggcgat gttgtctggg cgttgcttgc
    14101 cgtctcactg gtgcgccaca cggatggcac gccgctctat tttatcgctc agattgaaga
    14161 cattaacgag ctaaaacgca ccgaacaggt gaatcagcaa ctgatggagc gcatcacgct
    14221 ggctaacgaa gcgggcggga ttggcatctg ggagtgggag ctgaagccga atatttttcg
    14281 agctgtatga aattcctccg catatcaaac cgaactggca ggtgtggtac gagtgcgtgc
    14341 tgccggaaga tcgccagcat gccgaaaaag tgattcgtga ttcgttgcaa tcacgctcgc
    14401 cctttaagct ggaatttcgc attaccgtaa aagacggtat tcgccatatc cgcgccctcg
    14461 ccaaccgggt actgaataaa gaaggcgaag tcgaacgcct gctcggcatt aatatggata
    14521 tgaccgaggt taaacagctt aacgaggcat tgtttcagga aaaagagcgc ctgcacatta
    14581 cgctggattc catcggtgaa gccgtggtct gtattgatat ggcgatgaaa attaccttta
    14641 tgaatccagt ggcggagaag atgagcggct ggacgcagga agaagcatta ggtgttccgc
    14701 tcctgacggt gttgcatatt acttttggcg acaacggacc attaatggag aacatttaca
    14761 gtgccgacac ctcacgttcc gcgattgaac aagatgtggt gttgcactgc cggaacggcg
    14821 gcagttacga cgtgcattac agtattacgc cgttaagtac tctggacggc agcaatatag
    14881 gctcggtact ggtgattcag gacgtcaccg aatcacgcaa aatgctgcgc cagctgagct
    14941 acagcgcctc ccatgatgca ctgacgcatc tcgccaatcg cgccagtttt gagaagcaac
    15001 tgcgtatcct gctgcaaacg gtaaacagta cacatcagcg acatgccctg gtgtttatcg
    15061 atcttgatcg ctttaaagcg gtgaatgaca gcgccgggca tgcggcgggt gacgctttac
    15121 tgcgcgaact ggcgtcgttg atgctgagta tgctgcgctc cagcgacgtg ctggcgcgac
    15181 tcggtggtga tgaatttggt ctactgttgc cagactgtaa tatcgaaagt gcgcgtttta
    15241 tcgctacacg cattatcagt gccgtgaatg attatcactt tatatgggaa ggccgtgtgc
    15301 atcgggtagg tgccagtgcc gggattacct tgattgatga caacaatcat caggcggcag
    15361 aagtgatgtc gcaggctgat atcgcctgtt atgcctccaa aaatggtgga cggggccggg
    15421 tgacggttta cgaaccacag caagctgccg cacatagcga acgggcggcg atgtcgcttg
    15481 atgaacagtg gcggatgatt aaagagaatc agttgatgat gatcgcccac ggtgtcgctt
    15541 cgccgcggat cccggaagcg cgtaatttgt ggctgatttc acttaagctc tggagttgcg
    15601 aaggcgagat tattgatgaa caaaaatttc gtcgtagctt cagcgatccg gcacttagcc
    15661 atgctcttga ccgacgggta ttccacgaat ttttccagca ggccgcaaaa gcggttgcca
    15721 gtaaaggcat aagcatcgcc ctcccccttt ccgttgccgg tttgagtagc gccacgctgg
    15781 tgaatgatct gcttgagcag ctgggaaata gccctctacc accacggtta ttacatctga
    15841 ttattccggc tgaagcgatt ttagatcacg cagaaagcgt gcaaaaactg cggctggtgg
    15901 gatgtcggat agtgctcagc caggtgggcc gcgatctgca aatcttcaac tcgctgaaag
    15961 cgaatatggc agattacctg ctacttgatg gtgagttatg cgccaacgtg cagggtaatt
    16021 tgatggatga gatgctgatt acgattattc aggggcacgc tcagcgactc gggatgaaaa
    16081 ccatcgccgg gccagtcgtt ttacccttag tgatggatac gctttctggc atcggcgtcg
    16141 atctgattta tggtgatgtg attgccgatg cccaaccgct ggatttgctg gtgaatagca
    16201 gttatttcgc gattaactga gagagcgttt gcccattgcc tgatgcgacg tatcaggcct
    16261 acaattcgtc cgttccgtct ggttgccagc cttccgtata ccagatatgc aacagcgcat
    16321 aagaacgcca gggcttccag cgctcggcat aacggcggat ttgtgccggt gtcatccccg
    16381 gaaatcgctg tttaatcaga taatcatccg gcagaaaaac atctttcgcc tgccagccac
    16441 gcaaagcgaa ataattcgcc gtccagcgcc cgatacccgg aaaagtttgc agcgttttca
    16501 tcgcctgctc cacatcgccc ggaattgtca ttggtaagct gccctccagc gccgcatttg
    16561 ccagatgaat cagtgcctct gcccgtttca acggcatgcc taacgctttt aatgcctgcg
    16621 ggtcggctgc tgccagccgc tgaggcgtgg ggaagcagat atactccgga aaatcatcca
    16681 gccgttcgcc ataaagctgt acaactttgg cagtcaattt tgccgccatc gccacgctca
    16741 ccagttggcc taaaatcgcc cgcacaccct gctcaaaagc atcaatacag ccgggtaaac
    16801 gcaatccggg ccgcgccgcg cctaacttgc ccaacgcacc gttaacaatc tgcgggttac
    16861 attgcagatc aaacaggcgg ctcattttcg ccagacactc agcggcaacg ggttctaaac
    16921 ctgcacttaa atttatgtgc agagtatggc gggctatatc cggaatagca gtcaccacgc
    16981 cgcgatattc gcccaccgcc agactacggg cataataatc gtcagcgacc gtttccacac
    17041 cactcactgc acgggcggcg agaaatccca acatccacga ccagtcatac ggcggctgcc
    17101 agttcagggt atacatcgca tctccttatt catccgcttt cagcataaac gttattcaga
    17161 cgctacgctt tgctttcata ttccggttgt cgcgacggca acatttcgct aaagtcacgc
    17221 cccttcttca ccggcatggg gattatttcg tgtttattgg ttttgattac ggtacagcaa
    17281 actgttcagt ggcggtcatg cgtgacggta aaccgcactt gctaaaaatg gaaaacgaca
    17341 gcacgctgct gccttcaatg ctttgcgcgc caacgcgtga agcggtaagc gaatggctgt
    17401 accgccatca tgacgttccg gcagacgacg atgaaacgca ggcgttgtta cgacgggcga
    17461 ttcgttataa ccgcgaagaa gatatcgatg ttacagcgaa aagcgtgcag ttcggtcttt
    17521 cctcactggc acagtacatt gatgatccag aagaagtgtg gtttgtgaaa tcaccaaaat
    17581 cgttcctcgg tgccagcggc ttaaaaccgc agcaggtagc gctgtttgag gatctggtct
    17641 gcgcaatgat gttgcacatt cgccagcagg cgcaggcgca gctgccagaa gcgattactc
    17701 aggcggtgat tggtcgcccg atcaacttcc aggggctggg cggcgatgaa gcaaacgccc
    17761 aggcgcaagg gattctggaa cgcgcggcga agcgtgccgg attcagggac gtggtattcc
    17821 agtatgagcc ggtcgcggct gggctggatt acgaagccac cttgcaggaa gaaaaacggg
    17881 tgctggtggt ggatatcggt ggtggtacga ctgactgttc attgctgctg atgggaccgc
    17941 agtggcgttc gcgtctcgat cgtgaagcca gcctgctggg tcacagtggt tgtcgtattg
    18001 gcggtaacga tctggatatc gccctggcgt ttaaaaacct gatgccactg ctgggtatgg
    18061 gtggcgaaac cgaaaaaggc atcgccctgc cgatcctgcc gtggtggaat gcggttgcca
    18121 tcaacgacgt acctgcgcag agtgatttct acagtagtgc caacggtcgt ctgcttaacg
    18181 atctggtacg cgatgcccgc gaaccggaaa aagtggccct gttacagaaa gtctggcgtc
    18241 agcgtttaag ctatcgcctg gtacgcagcg cagaagagag caaaatcgcc ctttcaaacg
    18301 ctgcggaaac ccgcgcctca ctgccgttta tcagcgatga actggctacg ctgattagcc
    18361 agcaagggct ggaaagcgcc ctcagccagc cactggcgcg gattctggaa caggtgcaac
    18421 tggcgctgga taacgcccag gaaaaaccgg acgttatata cctgaccggc ggtagcgccc
    18481 gttcgccgct gattaaaaag gctctggcag aacagttgcc gggcattccg attgcaggcg
    18541 gcgatgactt tggctccgtc accgccgggc tggcacgctg ggcggaagtg gtatttcgtt
    18601 aaaagaaaat ggctcagcag cggcttaaat ctgcctgcgc ctgagccagt ttttctgccg
    18661 cctgacgtaa cggttccatc aacggcatgg tccgtgctgg agcataaagg cggaatcgct
    18721 gcaactctgt cgcgccgaca gtcaacgccg attccagccg atgtcgtttg gcggccatta
    18781 ccgcctgtct gtctgctggc gttaccgctt cgttcggcct gaacacaaaa cggcggtcac
    18841 agctcgcttt ccagtcgata accgcctggg tcagatggtc gccaaaccct ttaacttgct
    18901 taacgccacg acgggtaaca tccgctgctg tttcaatacc aaaagaccgt aacgccgctt
    18961 tacgcgcagg gccaacgccg ggaatagagg caaaaaatcc ctccagaaac ttctgcttct
    19021 gccgttccct tgcggtgtca taaagtgctg ccagagcgcg tttctcctct tccggtaacc
    19081 cgagaatttc gtccttcatt ttttcgagca tcgcccgttt ggcgataaaa ccttccagcc
    19141 cgcccaactg ttggatctgg ctgactaaat gctcatagtc cattttcgcg cgattaaatg
    19201 cctcacggcg ttgctggatt tctgctttgt acactttgct tgtcagactg ccaataatcc
    19261 agatagccga cagcacagga accaaaataa tataacgcgg ttctgcctga aggccgcaaa
    19321 gcaacagcga taacgctgac agtgcgatct caatcagaat gatgtattcg cgccgtaaca
    19381 ggcctaaagg aagcggcctg ccagtcggtt ggaaatgatc gggtaatggc aattgcaatg
    19441 ctggcggtgc tactgacgcc atcaccatcg cccagacttt cgccagcaca aaatcaccgc
    19501 tggtggtaat gacctcttca ccgagatcaa caaaatagat aacgccttga ttatccagcg
    19561 tacaccacgg gcagtcggtc aaatgagcgg gataaacatg cattgccgaa acgggacatt
    19621 tctttaactg ttggcgcaga gaatcaagtg ccgctaccca cgctttagcc gtcggacgcc
    19681 cggttgccac gccactttcc gtgaatgcct gctgaaacat ggcttcaaca tcactcggta
    19741 acatcgatag cggaatcgat cgtggcggcg gttttaaacc acgtcgctga ttatctgacg
    19801 cgtaggcata acggaaatgg gtaatatccg tctccagcgc attacccgca tcagagataa
    19861 gcggcacacc agaataagga tgccggccac caaacaagac gtgaaaaatc agcaacgcaa
    19921 ggccaaaatt atcgtgattt tctgtgcgtt caaagccgac aaatgacgac aaggtttgta
    19981 gctctggcgg cgtaaaatgc gacacgccga cttcgcataa atgcagtgtg ccattgacgt
    20041 taatctgaaa ggagtcgcta tcaatcagta ccactttgct gtcgcgacct accataaaac
    20101 tgttctggtt tacgtcaccc acgacgtgcc cgtgctcgtg aaccgtagca aaagatgaag
    20161 caatattgcg cgcaacatag agtagaaaat cccacgcaca atgagggtaa ctctggcgac
    20221 gatgtgccgg gctatagatc atatgaatcg gttctttacc agaaactttt ggcatcataa
    20281 aaccgataac ttttccgcct cgtccaccgt gaagcgttgc ctgcggccag gcgacataat
    20341 tcaacaactg cgcgtcagct gtcgcagcca taaaggcaag tttgtcctgt tttgaggcgg
    20401 gtggcggcgt gtgataaatc ttggcgacgc tatcgacaaa ctcctcgata tcataaaccg
    20461 cgccttcacc gcctttgccc agttcacggc ctggcgtaac gcactcacca gtagcagtat
    20521 ataaagtggg tttcatggta accacagcgc cagtgccaac gttttatcat catccgtacg
    20581 ctcgtttacc gccgggctgg acaaaaactg ctttaacaat tcaggtaata aatcgagttg
    20641 ttcctgcgtt actgacgcca gtccattgaa aaacggggta aagaaaggca catgaggaga
    20701 attatccagc atatttaacg ccagccgctg aataccatcc gtaaatgctg caactttatg
    20761 cgcacgccca gtgctggtga aagtttccag tctggaaacg gcatcttcgt cggtaatgaa
    20821 gtgcgtcatg ttggcatatt cacccaccat cggcgtgaga ggcaattgca gaccgtgacc
    20881 caaatctacc accacgccgc catcgccaat ctgcataatc aaggtgccgt caggcgatga
    20941 aatcaatccc agaaacgtgc aggcaaaatc gcgaactgct aatgctttgg cttctgcctc
    21001 ggcaaaaagt cgttgacgaa tagtcagtac catatttgtg gcgaggacat cattaagccc
    21061 caattcaccg tcctgtactt tttgcaccat aaacgccatc gcttcattga cggcgagcgt
    21121 cgcgccttct cctccctgcg aaacgctacc tgcgccatca gcaaggaaca tcaccagtaa
    21181 tggttgctgg tcgtttaacc agacaacctg catctggcag gcatcctgac aaggcaaatc
    21241 agcactaatg tgcgatgttc caacagcgga tgcataaacc agacgccagc tcacactgac
    21301 gtccagcctt ttggcgcttc cagcacaact tccgttcccg gcgtggagcg ggaaaccgaa
    21361 cgcagcgaac tggataacca gctaaacagt tcacggaatt gtaatccctg cagtggcaaa
    21421 ggctgacgaa cgctgatttg tgccagagtc ttcatatccg ccccctgaac accgatggaa
    21481 aagaaggcaa acttcttatc ttcttccccc tgaaacactt tgttggcggc agcctgccac
    21541 tcatcggttg gagcgccatc ggtgatcagg aaaatccacg ggcgataata cgaaatacca
    21601 tttgcgcggt actcacgttt cctctcttca accatatcta gggctttggt aatggcggcc
    21661 cccataggcg tatccccctg ggcaaacagg atgggcggga aaaagttagc ggcgctggta
    21721 aagggttgtt ccacatgcac cgggccgaac gtcacaatgc cgagttccac tctttttaaa
    21781 gccagcggat cagcaagcag ttcatcacga aaggtaacca atccggcgtt aagttcatta
    21841 ataggtctgc cgctcatcga gccagaaaca tccagcagca aaatgcaggg gcaacgtggt
    21901 tccgggttac tggcaaaatc gctggtggca aacgtgattt gttcgctcat aagaaatcct
    21961 ttcttagaga attatcgagg tccggtatca taccgattgc aataatgaaa taagagcaaa
    22021 cgaagaaact ttcagaatta ggcccgtcgg cgcagaaaat tttgctattc gcctcgcaag
    22081 aacggagagc cgtcgattga cactaagggc ggagtgacat aatttcagga gtgagggtta
    22141 gggagaggtt tccccctccc cctggtgttc ttagtaagcc tggaagctaa tcactaagag
    22201 tatcaccagt atgatgacgt gcttcatcat aaccctttcc ttattaaaag ccctcttctc
    22261 cgggagaggc tttcccgttt cagcgtcccg ctgaaatcat cggcttacct cctttcgccc
    22321 tgaatgcagt ctatcgctaa cgcgtgaata aaatagtttc ctctgtgatt attgcgtaac
    22381 gactcgcaaa gaatatttgc tggcaggatc gcagactaca aagcctacgg attgacaatc
    22441 ttatcgtgaa ggcatacttt caggagtgag ggtagagcgg ggtttccccc gccctggtag
    22501 tcttagtaag cggggaagct tatgactaag agcaccacga tgatgagtag cttcatcatg
    22561 accctttcct tatttaaggc cccttcctcg ggaggggctt tcccgtttca gcgtcccgct
    22621 gaaatcgtcg gcttacctcc tttcgccctg tatgcagtct atcgctgact tgcggataaa
    22681 atagtttcca gtgtgattat tgcgtaacgt ctcgcaaaga atatttgctg gcaggatcgc
    22741 agactgcaaa gcctgcagat tgacaatctt atcgtgaagg catactttca ggagtgaggg
    22801 tagagcgggg tttcccccgc cctggtagtc ttagtaagct ggccagctaa tgactaagag
    22861 caccacgatg atgagtagct tcatcatgac cctttcctta tttatggccc cttcctcggg
    22921 aggggctttc ccgtttcagc gtcccgctga aatcggcggc ttacctcctt tcgccatgca
    22981 agcagtctat ccctaacgcg tagataaaat agtttcctgt gttattactg gatgcgtgct
    23041 cgcaaatgtg cccgtcattc agacaattcc cgacagtgtt tcataattcc tccatttttc
    23101 tcccttattg gctggctaca ctagtatcat tccgcgaaac gtttcaggaa gagaaactct
    23161 taacgatgaa aggcagttat aaatcccgtt gggtaatcgt aatcgtggtg gttatcgccg
    23221 ccatcgccgc attctggttc tggcaaggcc gcaatgactc ccggagtgca gccccagggg
    23281 cgacgaaaca agcgcagcaa tcgccagcgg gtggtcgccg tggtatgcgt tccggcccat
    23341 tagccccggt tcaggcggcg accgccgtag aacaggcagt tccgcgttac ctcaccgggc
    23401 ttggcaccat tatcgccgct aatactgtta cggtgcgcag ccgcgtggac ggtcaactga
    23461 tggcgttaca tttccaggaa ggccagcagg tcaaagcagg cgatttactg gcagaaattg
    23521 accccagcca gttcaaagtt gcattagcac aaacccaggg ccaactggca aaagataaag
    23581 ccacgcttgc taacgcccgc cgtgatctgg cgcgttatca gcaactggca aaaaccaatc
    23641 tcgtctcccg tcaggagctg gatgcccaac aggcgctggt cagtgaaacc gaaggcacca
    23701 ttaaggctga tgaagcaagc gtcgccagcg cgcagttgca actcgactgg agccgcatca
    23761 ccgcaccagt cgatggtcgc gttggtctca agcaggttga tgttggtaac caaatctcca
    23821 gtggtgatac caccggaatt gtggtgatca cccagacgca tcctatcgat ttagtcttta
    23881 ccctgccgga aagcgatatc gctactgtag tgcaggcgca aaaagccgga aaaccgctgg
    23941 tggtagaagc ctgggatcgc accaattcga agaaattaag tgaaggcacg ctgttaagcc
    24001 tcgataacca aatcgatgcc acaaccggta tgattaaagt gaaagcacgc tttaataatc
    24061 agaatgatgt gctgtttccc aatcagtttg ttaacgcgcg catgttagtc gacaccgaac
    24121 aaaacgccgt agtgatccca acagccgccc tgcaaatggg caatgaaggc cattttgtct
    24181 gggtgctgaa tagcgaaaac aaggtcagca aacatctggt gacgccgggc attcaggaca
    24241 gtcagaaagt ggtgatccgt gcaggtattt ctgcgggcaa tcgcgtggtg acagacggca
    24301 ttgatcgcct gaccgaaggg gcgaaagtgg aagtggtgga agcccagagc gccaccactc
    24361 cggaagagaa agccaccagc cgcgaatacg cgaaaaaagg agcacgctcc tgatgcaggt
    24421 gttacccccg agcagcacag gcggcccgtc gcgcctgttt attatgcgtc ctgtcgccac
    24481 cacgctgctg atggtggcga tcttactcgc cgggattatc ggttatcgcg ccctgcccgt
    24541 ttcggcgctg ccggaagtgg actatccgac cattcaggtg gtcacgctct acccaggtgc
    24601 cagcccggat gtcatgacct ctgccgttac cgcgccgctc gaacgccagt tcgggcagat
    24661 gtctggcctg aaacagatgt cgtcgcaaag ttccggcggt gcgtcagtta tcactttgca
    24721 gttccagcta acattaccgc tcgatgtcgc cgagcaggaa gtgcaggccg cgattaacgc
    24781 tgcgaccaac ttgttgccga gcgatctgcc taacccgccg gtttacagca aagtgaaccc
    24841 ggcagatccg ccgatcatga cgctcgccgt cacctcaacc gccatgccga tgacgcaagt
    24901 ggaagatatg gtggaaaccc gcgtcgcgca gaaaatctcg cagatttccg gcgtcggcct
    24961 ggtgacgctt tccggcggtc agcgtccggc tgttcgcgtc aaacttaacg ctcaggcgat
    25021 tgccgccctc ggcctgacca gcgaaaccgt gcgcaccgcc attaccggcg ctaacgttaa
    25081 ctcggcaaaa ggtagcctcg acggcccttc ccgtgcggtc acgctttccg cgaacgacca
    25141 gatgcaatcc gccgaagagt atcgccagct aatcatcgcc taccagaacg gcgcgccaat
    25201 tcgtctgggc gatgtcgcaa ctgtagagca aggtgcagaa aacagctggc tcggcgcgtg
    25261 ggcgaacaaa gaacaggcca ttgtgatgaa tgttcagcgc cagcccggtg ctaacattat
    25321 ctccaccgcc gacagcattc ggcagatgct gccacagctc actgagagtc tgccgaaatc
    25381 ggtgaaggtg acagtgcttt ccgatcgcac caccaatatc cgcgcatccg tcgatgatac
    25441 tcagtttgaa ttgatgatgg ctatcgcgct ggtagtcatg attatctacc tgtttttgcg
    25501 caatattccg gcgaccatca ttcccggtgt tgctgtaccg ctgtcgttaa tcggcacttt
    25561 cgcggttatg gtgtttctcg atttttcaat caataacctg acactgatgg cgttaactat
    25621 cgccaccgga ttcgtggtcg atgacgccat cgtggtgatc gaaaacattt cccgctatat
    25681 cgaaaaaggc gaaaaaccgt tggcggcggc gctcaagggc gcaggtgaaa tcggctttac
    25741 cattatctcg ctgatcttct cactgattgc ggtgttgatc ccactgctgt ttatgggcga
    25801 tatcgtcggg cgactgttcc gcgaatttgc tattaccctg gcggtagcga ttttgatctc
    25861 agcggtggtg tcgctgaccc tgacaccgat gatgtgcgcg cggatgctca gccaggagtc
    25921 gttgcgtaaa cagaaccgct tctcccgtgc ctcggaaaaa atgttcgaca ggataatcgc
    25981 cgcctatggt cgtggactgg cgaaagtgct gaatcatccg tggctgacct taagcgtggc
    26041 actcagcacg ctgctgctta gcgtgctgct gtgggtgttc attccgaaag gtttcttccc
    26101 ggtacaggac aatggcatta ttcagggcac tttgcaggca ccgcaatcca gctcctttgc
    26161 caatatggcc cagcgacaac gccaggtcgc ggacgtgatt ttgcaggatc cggcagtgca
    26221 aagcctgacc tcatttgttg gcgttgatgg cactaacccg tcgctgaata gtgcacgttt
    26281 acaaatcaac ctcaaaccgt tggatgaacg tgatgatcgg gtgcaaaaag tcatcgcccg
    26341 tctgcaaacg gcggtagata aagtgccggg cgtcgatctc ttcctgcaac caacgcagga
    26401 tctgactatt gatactcagg tcagccgcac ccagtaccag tttaccttgc aggccacctc
    26461 actggatgcg ctcagtacct gggtgccaca gttgatggaa aaactccagc aactgccaca
    26521 gctttctgat gtctccagcg actggcagga caaagggctg gtggcgtatg tcaatgttga
    26581 tcgcgacagc gccagccgtc tggggatcag catggcggat gtcgataatg ccctgtacag
    26641 cgcgtttggt cagcgactga tttccactat ttatactcag gctaaccagt atcgcgtagt
    26701 gctggagcac aacaccgaaa ttaccccggg cctcgcgacg ctggatacca ttcgcctgac
    26761 cagtagcgac ggcggcgtgg tgccgttaag ctcaattgcc aaagttgaac agcgttttgc
    26821 gccgctttcc atcaaccatc tggatcagtt cccggtaacg accatctcct ttaacgtgcc
    26881 ggataactat tcgctgggcg atgcggtgca ggcgattatg gacaccgaaa agacgctgaa
    26941 tctgccggtg gatatcacca cgcaattcca gggcagcacc ctcgccttcc agtcggcgct
    27001 gggcagcact gtctggctga ttgtcgcggc ggtggtggcg atgtatatcg tgctcggcat
    27061 tctgtacgag agctttattc acccgatcac cattctctcg acgctaccca ccgcaggggt
    27121 cggcgcgctg ctggcattaa tgattgccgg tagcgaactg gatgtgattg cgattatcgg
    27181 cattattttg ctgatcggta ttgtgaagaa gaacgccatc atgatgatcg acttcgcgct
    27241 ggctgctgag cgcgagcaag gcatgtcgcc gcgcgatgca atctaccagg cttgtctgtt
    27301 gcgttttcgt ccgatcctga tgaccaccct ggcggctctg cttggcgcgc tgccgctgat
    27361 gttgagtacc ggggtcggcg cggaactgcg tcgtccgtta ggtatcggca tggtcggcgg
    27421 tctgattgtc agccaggtgc tgacgctgtt caccacgccg gtgatttatc tgctgttcga
    27481 ccgcctggca ttgtggacca aaagtcgctt tgctcgccat gaagaggaag cgtaagtgaa
    27541 gttttttgcc ctcttcattt accgtccggt ggcgacgatt ttactgtcgg ttgccattac
    27601 cctgtgcggc atactgggct tccgtatgct gccggtcgcc ccgctgccgc aggtcgattt
    27661 tccggtgatt atggtcagcg cctcgctgcc cggtgcgtca ccagaaacaa tggcgtcttc
    27721 cgttgccacg ccgctggagc gctcacttgg gcgcattgcc ggagtcagtg aaatgacctc
    27781 cagcagttcg ctcggcagca cgcgtattat tttgcagttt gattttgacc gggatatcaa
    27841 cggcgcggcg cgcgatgtgc aggcggcgat caacgctgca caaagtttgc tgcccagcgg
    27901 gatgcccagc cgtccgacct atcgcaaagc taacccgtcg gatgcgccaa ttatgatcct
    27961 cacgctgaca tccgatactt attcgcaggg taaactgtac gatttcgcct cgacgcagct
    28021 ggctccgacg atttcgcaaa tcgacggtgt tggtgatgtc gatgtcggcg gcagctcact
    28081 gcccgccgta cgcgtcgggc tgaatccgca ggcgctgttt aatcagggcg tctcgctgga
    28141 cgacgtacgc actgccatca gcaatgccaa cgtgcgtaaa ccgcaggggg cgctggaaga
    28201 tggcactcac cgctggcaga tccagaccaa tgatgagcta aaaaccgccg ccgaatatca
    28261 gccgctgatt attcattaca acaacggcgg cgcggttcgt ctgggcgatg tggcgacagt
    28321 gaccgactct gtacaggatg tacgtaacgc cgggatgacc aatgccaaac cggctatttt
    28381 gctgatgatc cgcaagctgc cggaagccaa tattatccag acggttgaca gcatccgggc
    28441 aaaattaccg gagttgcagg aaaccattcc ggcggcgatt gatctgcaaa ttgctcagga
    28501 tcgcgccccc accattcgcg cctcgctgga agaagtcgag caaacgctga ttatctcggt
    28561 ggcgctggtg attctggtgg tgtttttatt cctgcgctcg ggtcgcgcaa ctattattcc
    28621 cgccgttgcg gtgccggttt cgctgattgg tacgtttgcg gcgatgtacc tgtgcggttt
    28681 cagtctcaat aatctttcgt taatggcgct caccatcgct actggtttcg tggtggatga
    28741 cgccatcgtg gtgctggaaa acattgcccg tcatctggaa gcgggaatga aaccgttgca
    28801 agccgcactg caaggtactc gcgaagtcgg ttttacggtg ctgtcgatga gtctgtcact
    28861 ggtggcggtg ttcctgccgc tgctgttgat gggcggattg ccgggccgac tgttacgcga
    28921 atttgccgtg acgctttctg tcgccattgg tatatcgttg ctggtttctc tgacattaac
    28981 gccaatgatg tgtggctgga tgctgaaagc cagcaagccg cgcgagcaaa agcgactgcg
    29041 tggttttggt cgcatgttgg tagccctgca acaaggctac ggcaagtcac tgaaatgggt
    29101 gctcaatcat acccgtctgg tgggcgtggt gctgcttggc accattgcgc tgaatatctg
    29161 gctgtatatc tcgatcccga aaaccttctt cccggagcag gacactggcg tgttgatggg
    29221 cgggattcag gcggatcaga gtatttcgtt tcaggcgatg cgcggtaagt tgcaggattt
    29281 catgaaaatt atccgtgacg atccggcagt ggataatgtc accggcttta caggcggttc
    29341 gcgagtgaac agcgggatga tgtttatcac cctcaagcca cgcgacgaac gcagcgaaac
    29401 ggcgcagcaa attatcgacc gtctgcgcgt aaaactggcg aaagaaccgg gggcgaatct
    29461 gttcctgatg gcggtacagg atattcgcgt tggtgggcgt cagtcgaacg ccagctacca
    29521 gtacacgttg ttatccgacg acctggcggc actgcgagaa tgggagccga aaatccgcaa
    29581 aaaactggcg acgttgccgg aactggcgga cgtgaactcc gatcagcagg ataacggcgc
    29641 ggagatgaat ctggtttacg accgcgacac catggcacgg ctgggaatcg acgtacaagc
    29701 cgccaacagt ctgttaaata acgccttcgg tcagcggcaa atctcgacca tttaccagcc
    29761 gatgaaccag tataaagtgg tgatggaagt ggatccgcgc tatacccagg acatcagtgc
    29821 gctggaaaaa atgttcgtta tcaataacga aggcaaagcg atcccgctgt cgtatttcgc
    29881 taaatggcaa ccggcgaatg ccccactatc ggtgaatcat cagggattat cggcggcctc
    29941 gaccatttcg tttaacctgc cgaccggaaa atcgctctcg gacgccagtg cggcgatcga
    30001 tcgcgcaatg acccagcttg gggtgccttc gacggtgcgc ggcagttttg ccggcacggc
    30061 gcaggtgttc caggagacga tgaactcgca ggtgatcctg attatcgccg ccatcgccac
    30121 ggtgtatatc gtgctgggta tcctttacga gagttacgta catccgctga cgattctctc
    30181 caccctgccc tcggcgggcg ttggagcgct gttggcgctg gagctgttca atgccccgtt
    30241 cagcctaatc gccctgatag ggatcatgct attaatcggc atcgtgaaga aaaacgccat
    30301 tatgatggtc gattttgcgc ttgaagccca acggcacggt aacctgacgc cgcaggaagc
    30361 tattttccag gcctgtctgc tgcgttttcg cccgattatg atgaccaccc tggcggcgct
    30421 gtttggtgcg ctgccgctgg tattgtcggg cggcgacggc tcggagctgc ggcaacccct
    30481 ggggatcacc attgtcggcg gactggtaat gagccagctc cttacgctgt ataccacgcc
    30541 ggtggtgtat ctctttttcg accgtctgcg gctgcgtttt tcgcgtaaac ctaaacaaac
    30601 ggtaaccgag taaatgacag atcttcccga cagcacccgt tggcaattgt ggattgtggc
    30661 tttcggcttc tttatgcagt cgctggacac caccatcgta aacaccgccc ttccctcaat
    30721 ggcgcaaagc ctcggggaaa gtccgttgca tatgcacatg gtcattgtct cttatgtgct
    30781 gaccgtggcg gtgatgctgc ccgccagcgg ctggctggcg gacaaagtcg gcgtgcgcaa
    30841 tattttcttt accgccatcg tgctgtttac tctcggttca ctgttttgcg cgctttccgg
    30901 cacgctgaac gaactgttgc tggcacgcgc gttacagggc gttggcggcg cgatgatggt
    30961 gccggtcggc agattgacgg tgatgaaaat cgtaccgcgc gagcaatata tggctgcgat
    31021 gacctttgtc acgttacccg gtcaggtcgg tccgctgctc ggtccggcgc tcggcggtct
    31081 gctggtggag tacgcgtcgt ggcactggat ctttttgatc aacattccgg tggggattat
    31141 cggtgcgatc gccacattgc tgttaatgcc gaactacacc atgcagacgt ggcgctttga
    31201 tctctccgga tttttattgc tggcggttgg catggcggta ttaacccctg gcgctggacg
    31261 gcagtaaagg tacaggttta tcgccgctgg cgattgctgg cctggtcgca gttggcgtgg
    31321 tggcactggt gctttatctg ctgcacgcca gaaataacaa ccgtgccctg ttcagtctga
    31381 aactgttccg tactcgtacc ttttcgctgg gcctggcggg gagctttgcc ggacgtattg
    31441 gcagtggcat gttgcccttt atgacaccgg ttttcctgca aattggcctc ggtttctcgc
    31501 cgtttcatgc cggactgatg atgatcccga tggtgcttgg cagcatggga atgaagcgaa
    31561 ttgtggtaca ggtggtgaat cgctttggtt atcgtcgggt actggtagcg accacgctgg
    31621 gtctgtcgct ggtcaccctg ttgtttatga ctaccgccct gctgggctgg tactacgttt
    31681 tgccgttcgt cctgttttta caagggatgg tcaactcgac gcgtttctcc tccatgaaca
    31741 ccctgacgct gaaagatctc ccggacaatc tggcgagcag cggcaacagc ctgctgtcga
    31801 tgattatgca attgtcgatg agtatcggcg tcactatcgc cgggctgttg ctgggacttt
    31861 ttggttcaca gcatgtcagc gtcgacagcg gcaccacaca aaccgtcttt atgtacacct
    31921 ggcttagcat ggcgttgatc atcgcccttc cggcgttcat ctttgccaga gtgccgaacg
    31981 atacgcatca aaatgtagct atttcgcggc gaaaaaggag cgcgcaatga agttctggcg
    32041 acccggtatt accggcaaac tgtttctggc gattttcgcc acctgcattg tcttgctgat
    32101 cagtatgcac tgggcggtgc gtatcagttt tgagcgtggc tttattgatt acatcaagca
    32161 tggtaatgaa cagcgattac aactgttaag tgatgcgctt ggcgagcagt atgcgcagca
    32221 tggcaactgg cgcttcctgc gcaacaatga tcgctttgtc tttcagatcc tgcgttcatt
    32281 tgaacacgat aattcggaag ataaacccgg cccgggtatg ccaccgcacg gctggcgtac
    32341 ccagttctgg gtggttgatc aaaacaacaa agtgctggtt ggtccgcgag cgccgattcc
    32401 acctgacggt acacggcgac ccattctggt caacggtgcg gaagttggcg cggtgatcgc
    32461 ctcccccgtt gagcggttaa cgcgcaatac tgatatcaat ttcgataaac aacagcggca
    32521 aaccagctgg ttgattgtcg ccctggcaac gttactcgcg gcacttgcca cttttctgct
    32581 ggcgcgcggt ttactggcac cggtaaaacg acttgtcgat ggcacgcaca aactggcggc
    32641 gggcgatttc actacccgcg taacgcccac cagtgaagat gaactgggca aactggcgca
    32701 agacttcaac cagcttgcca gcacactgga gaaaaaccag caaatgcggc gcgattttat
    32761 ggccgatatt tctcacgaac tgcgtacgcc attagcggtg ctgcgcggtg aactggaagc
    32821 cattcaggat ggcgtgcgta aattcacgcc ggagacggtg gcgtctttac aggcggaggt
    32881 cggtacactg accaaactgg ttgacgatct ccatcagttg tcgatgtctg atgaaggcgc
    32941 tctcgcctat caaaaagcac cggtagattt gatcccactg ctggaagtgg cgggcggcgc
    33001 atttcgcgaa cgattcgcca gtcgtggcct gaaactgcaa ttttccctgc cagacagtat
    33061 taccgtattt ggcgatcgcg accgtttaat gcagttattc aataatttac ttgaaaacag
    33121 cctgcgctat actgacagcg gcggcagcct gaaaatctct gccgagcagc acgacaaaac
    33181 ggtgcgcctg acctttgccg acagtgcgcc aggtgtcagt gacgagcagc tacaaaaatt
    33241 atttgaacgt ttttatcgca ccgaaggttc ccgcaaccgc gccagcggtg gttccgggct
    33301 ggggctggcg atttgcctga acattgttga agcacataat ggccgcatta ttgctgccca
    33361 ttcgcctttt ggcggggtaa gcattacagt agagttaccg ctggaacggg atttacagag
    33421 agaagtatga ccgagttacc aatcgacgaa aacacaccac gtattttgat cgtggaagat
    33481 gaaccgaagc tggggcagtt gctcattgat tatctgcgtg ctgcgagcta tgcgccgacg
    33541 cttatcagcc acggcgatca ggtactgccg tatgtgcgcc agacaccacc ggatctgatc
    33601 ctgttggatc tgatgctccc tggcaccgat ggcttgacgc tgtgccggga aattcgtcgt
    33661 ttttctgaca ttcctatcgt gatggtgacg gcaaaaatcg aagagatcga ccgcctgctc
    33721 ggactggaga ttggcgcaga tgactacatc tgcaagccgt acagcccacg ggaagtggta
    33781 gcgcgcgtca aaaccatttt gcgccgttgc aaaccgcagc gcgaattgca gcaacaggat
    33841 gccgaaagcc cgttgattat cgacgaaggt cgttttcagg cttcatggcg cggtaaaatg
    33901 cttgacctga cacctgcgga atttcgtctg ttgaaaacgc tctctcatga accaggaaaa
    33961 gtgttctccc gcgagcaatt gctcaatcat ctttatgacg actaccgcgt agtaaccgac
    34021 cgcaccattg acagccacat taaaaacctg cgccgcaagc tggaatctct cgacgccgaa
    34081 cagtcattta tccgcgccgt ttacggcgtt ggttaccgct gggaagccga cgcctgccgc
    34141 atcgtttagt tttagcgaca tgactttgtt agccggagat ggcgttcaga ccagatccgg
    34201 caacattatc ccacgcatgg tcagcaaact gacattatct ctccgccctg gcaggcatca
    34261 gaaaatcgct catactttaa tcggtaaaca gcacctttag atgctgtttt cgatacgcaa
    34321 tttcaatcaa ggagtcatta tggctggttg gtttgaactc agtaagagca gtgataatca
    34381 gttccggttt gtgctaaaag cgggcaatgg tgagactatc ctcaccagcg agctgtatac
    34441 atcaaaagcc tctgcggaaa agggcatcgc gtcagtgcgt agcaacagcc cgcaagaaga
    34501 acgctatgag aaaaaaacgg caagtaacgg caaattctat ttcaatctga aagccgccaa
    34561 tcatcaaatt atcggctcca gccagatgta cgccaccgcg caatctcgtg aaaccggaat
    34621 tgcctccgtt aaagccaatg gcacaagcca gacggtgaaa gacaatacgt aattacgatg
    34681 ccgggcgtgg gttgttggcg tccggcttta ttttctctca tcacagagtt actatccctc
    34741 aagaaattat taaccatgaa atgagttttc tcctcataac tgataaggaa aattatgctc
    34801 aaaggattgc gtacattatt ttcacgcagg gttttgttta acgtcgaaat gaataattct
    34861 gttgactgtg aattacttct catccagcaa caactccagg cattgatagc cgaaccaggc
    34921 aattatttag cacatgattg tgatctcaac gatactctgg ttctttgcca gcttacccat
    34981 catcacaatg gatatatacg agaaaaggcc gtgcagtatc tggggcacaa agaggatgtt
    35041 gcggctatag aagaattatt acttgcagct aatgactggg taaaagaggt tcgccttgct
    35101 gcaaccatag cattacgtca tttaatgaag gataagaatg cagcggcatt tgccgttaat
    35161 ctacccatga tacgtaacct tctccaatgc cagcgttacg atcaccgtgc atttgtagac
    35221 gagatactta gatatttatt gacagaaaat aaccgcccac aattaacgta ttggcttact
    35281 tgccctgata aaaagctctc ccgcgcggta ttacaaacgc tgattgaata cggttacttt
    35341 aatgatgaat cttcactgct ttcaatactc gataaacccg atgagggttt gcgaatgctg
    35401 gcgaccaccc actggctgcg tcaacaatta ccattaagcg aagcggtatt aactcgtctg
    35461 ttaaaagacc gctggccgcg tattcgacaa gcaacgttat ttagcctcac tgaccgagca
    35521 atagaaatgc ctccggcgtt gcacagcaca ttactgctgg ataacaatat gttgatccgc
    35581 ctacgcgcta aaaatatgct tcacgaagtc atggatgttc cgcaattctg gcgtcatgtt
    35641 gtcacttctg cggaatatac tccctcacag cgacgggccg ctttatacgg gctggattcc
    35701 attcatgatc ccaatatttt aaaactggca gaatggggat tatcacagaa tgtattccca
    35761 ctgcgtttag ccgctatgca cattcttgca aaagctaatc ctcgttgtgg cgtcaaagag
    35821 actattctga ccacgctggc taacccggac gccgccgggt tgcgatttat ggttaacatc
    35881 tgtgtatggt gtcgcgttcc gctcacgttc gaggaaatac gtcagttgca ggaaaacgcg
    35941 ccgtcagtta aacatgcttg cgcatattgt cggctgtatc ataacttgaa caaatgggac
    36001 ggtttaattc ttcttcttca atcgcaacac aagctaactg aagaatttgc aggtaaacag
    36061 ctcgcgatat ggcaacgcaa tttcaatctt tcgggcattc aaccgaatgc tcttcagcgt
    36121 cagcaacttc aggcgctttt tacccgcaat ccggaacttc acaacagatt atggggttac
    36181 attcctttta aataacttcg aattatagac ttacgatgac aaatgtacct gctgacgagc
    36241 ggaaccaaaa cgaaacaata cgtgaagctt tacgcttgct acgagaaaaa cttgccgagg
    36301 gactacgcag tggcgaagcc caaccgtggg aaaaggatct gtttttacat aaagtgaagg
    36361 ccggtaacaa ataaatcccc cagtcttttc cctcacattt gcactggagc caaccccgcc
    36421 gattgcgcta caatgcccgc ccttaaagtg ggggcactcc cctaaccgct tcatcaggtg
    36481 aagcggatct gacctgtcat cagaacgaga gaattatgtt taaaccggaa ctcctttccc
    36541 cggcgggaac gctgaaaaat atgcgttacg ctttcgctta tggcgcagat gctgtttatg
    36601 cgggccagcc gcgttactcc ctgcgtgtgc gcaacaacga attcaaccac gaaaatcttc
    36661 agctcggcat caatgaagcc cacgcgctgg ggaaaaagtt ttatgtcgtg gtcaacattg
    36721 caccgcacaa cgccaagctg aaaaccttta tccgtgacct gaaaccggtg gtggaaatgg
    36781 ggccggatgc gctgattatg tccgatccag ggctgattat gctggtgcgt gagtacttcc
    36841 ctgaaatgcc gatccacctc tcggtgcagg ctaacgccgt aaactgggcg acggtgaaat
    36901 tctggcagca aatgggcctg acccgcgtga tcctctctcg cgaactgtcg ctggaagaga
    36961 ttgaagagat ccgcaatcag gtgccggata tggagatcga gatcttcgtt cacggtgcgc
    37021 tgtgcatggc ctactccggt cgctgcctgc tctctggcta tatcaacaag cgcgatccga
    37081 accagggtac ctgcaccaac gcctgccgct gggagtacaa cgtccaggaa gggaaagaag
    37141 atgacgttgg caacatcgta cacaagtacg agccgatccc ggtgcaaaat gttgagccga
    37201 cgctaggtat cggcgcgcca accgacaaag tgtttatgat cgaagaagcc cagcgtccgg
    37261 gcgagtatat gaccgcgttt gaagatgagc acggcactta catcatgaac tcgaaagatc
    37321 tgcgcgccat cgcccatgta gaacgcctga ccaaaatggg cgtgcattcg ctgaaaatcg
    37381 aaggccgtac taaatctttc tactattgcg cacgcaccgc acaggtttat cgtaaagcta
    37441 tcgatgatgc cgctgcgggc aaaccgttcg ataccagcct gctggaaact ctggaaggtc
    37501 tggcgcatcg tggctatacc gaaggtttcc tgcgtcgtca tactcacgac gattatcaga
    37561 actacgaata cggttattca gtttctgacc gccagcagtt tgttggtgag tttaccggtg
    37621 agcgcaaggg ggacctcgca gcggtagcgg tgaaaaataa attctccgtt ggcgacagcc
    37681 ttgagctgat gacgccgcaa ggcaacatta actttaccct tgagcacatg gaaaacgcca
    37741 aaggtgtagc gatgccggtc gcaccaggcg atggttatac tgtgtggctc ccggtcccgc
    37801 aggatcttga actaaattac gcgctgctga tgcgtaattt ctccggggaa accacgcgta
    37861 acccacacgg taagtgatta atttcgatta tttttcccgg atggaaaatt cttagaaacc
    37921 gatcacatac agctgcattt attaaggtta tcatccgttt cgctgaaaaa cataacccat
    37981 aaaatgctag ctgtaccagg aaccacctcc ttagcctgtg taatctccct tacacgggct
    38041 tattttttat gcataagccc tatccctggt caccgtcttc cattgaccac atcgatagaa
    38101 tctcccttca tagcacgatg cctttcacgt aacggcatcg tgctcgcaca ggttccggct
    38161 aagcacaacc agaacgcgca tgtttgatgc ttaccaaaaa atattctcac tctccacatt
    38221 tgaatgtcag acgagcgaca ccatgtaatc ctacaccttc tgtcttcagc tcaactattt
    38281 gcattttttt gccctgagta acacagaaat gagttgcatc attttttact atattttctg
    38341 caccagatat tctactcctg gctaaagaag cttcggcttc ggtgtagtat tggttatcga
    38401 gtttacgctg aatattactt ttatatgcaa gaccaaattt accgatactt gtctcatcat
    38461 tatgcacagc acaaccagac ataataaaaa tactaattaa tgatatagca gctatctttt
    38521 tcatctcacc tttccccatt aaataccaac gacactctct agtgtttaaa tataataatg
    38581 gctgatcatt atatttgaat aggattataa taaatgttct gtacaacatt tcctacataa
    38641 gtaggactta cagacatgaa ggccctttcc aggtaactcc atggggcttt aatatatttg
    38701 gaatagtata tttaagatgc cactgtttgg ttgaatatta ggcatatgct cttttttgaa
    38761 atttatcggt ggctaccgtt aatattcgct gttcccattg caagctcctg gtggtaacca
    38821 ctgaatcctc catacttgaa ctgacttttt atcctccgac tttcatcctg ttctgactcc
    38881 accttttctt ttctgctcta cactatctac agaccaatca taaaggcaca tacgatcatg
    38941 gcagaatttc ccgccagctt actgattctt aatggcaaaa gtactgacaa tctacccttg
    39001 cgcgaagcaa ttatgctgtt gcgtgaggaa ggaatgacga tccatgtgcg ggtcacctgg
    39061 gagaaaggtg atgccgcacg atttgtagag gaggcccgga agttgggcgt cgcaacggta
    39121 attgccggtg gtggcgatgg caccattaat gaagtttcta cggcgttgat tcagtgtgag
    39181 ggggatggca tacccgcgct gggaattttg ccattaggaa ccgccaatga ttttgccacc
    39241 agtgtaggga ttcctgaggc actggataag gcgctgaaac tggcaattgc cggtaacgcc
    39301 attgcgatag atatggcgca ggtcaacaaa caaacctgtt ttattaatat ggcgacaggc
    39361 ggatttggga cgcgtattac cacagaaacg ccggaaaaat taaaagccgc gctgggtggc
    39421 gtctcttaca tcattcatgg cttaatgcgc atggatactc tgcaaccgga ccgttgtgaa
    39481 atccgcggtg aaaactttca ctggcaaggt gacgccctgg tattggtatt ggtaacgggc
    39541 gtcaggccgg tggcggtcag caattgtgtc cgaacgcgtt aattaacgat ggcttgctgc
    39601 aactgcgcat ttttaccggc gatgaaattc ttccggctct cgtatcaacc ttaaaatctg
    39661 acgaagataa cccgagtatt atcgaaggcg cttcgtcgtg gtttgatata caagccccac
    39721 acgaaatcac ttttaatctt gatggcgaac cgttgagcgg acaaaacttt catattgaaa
    39781 tacttccggc ggcgttgcgt tgtcgattac caccagattg tccattattg cgttaatcaa
    39841 actattctct cttgaaccag ggagaatccg aaatgccgga tgcggtgtaa ataccttatc
    39901 cggcatacag aacaatacta caccgtaatt aattttaccc gtgtcttctg caatgccagt
    39961 ttatccacct ctgatacagc atcatcggta ataataatgt caattttttc gattggcagt
    40021 acctgattaa aaccgcgacg gttaaatttc gatgaatcga gaacggcaat cactttatta
    40081 gcggctgtga ccatcgcacc gctaacggaa taaccctcat taaaggtcgt aataccatta
    40141 accgcatcaa taccatcagc accgacaaac attaaatcgg cattaatatc ctgcagggaa
    40201 cgttcagcaa tagatccatg catagatcgc gttttatggc ggaccgtgcc accgcagacg
    40261 accagagtaa tgtctttatt ttcggaaagg gcaaacgctg ccgggagact gttggtaatc
    40321 accgtgatat ttttagcggt cattaatcct tcggcgataa gcattgtggt acttccgctg
    40381 tcgagaataa cggtcatccc ttcatggatc attttgactg ccgcctgcgc tattctgttt
    40441 tttggcgcgc tggcgagctg aaatcgctct ttaaacccga cttcctgggt ctcggtttca
    40501 ctattaccgg acattatttt cgccgcaccg ccatgaaagc gcgtaaccac gcctttttgt
    40561 tcgagaaagc gcaaatcggc acggattgtc gcttccgagg cagcaaatac tcccgccaga
    40621 tcctgaacaa gcacggttcc ctgttcattc actaattgga tgatcttatt ccttcgctcg
    40681 aatgaattca tcgtcattta cccatattca attgtggcta gtgtaaacga agggggacca
    40741 ttggtgaatg atctgttttg cacaataccg ggccagcatc acgctggccc gcggtttcat
    40801 ggaatgagca acactttgcc cggcatggca ttacgagcga tgtcacgcac cgcctgggtg
    40861 aagctttcaa agcttccacg gtgagcgatt aatggctcca ggcttaactt acgttctgtc
    40921 agcaaccggc tcgccgtttc ccactcctgc cccggccaag ggctggaata gttcatccaa
    40981 ctgccgataa ccgtcagctc tttacgcaat attttgccaa acgttgccga tgttaaatgc
    41041 agatcctgat gcaacgtgcc caccagcgcc agttgggcat gaggaccggc aatctctacc
    41101 gccagttcga cagtttgcgg tacgccagcc gtctcgagga taagctgatt aaaacgcagt
    41161 tcgcgtaaaa cgctctgcat ttgcggcgcg ctcatttcgc tactgttaaa tgtttgcatc
    41221 gaaccgaaag attttgccag tgccagtttt tctgagctaa tgtcgatagc cgtcacactc
    41281 tttgctccca gcgcgacagc gcactgaata gccagcaggc caatggttcc ggcaccaata
    41341 ataataacgt ttttattctc acaaccttgc gctaaatgaa aagcatgcag accaatggta
    41401 atcggctcaa taaaagcccc atcctcaata ggcatatccg tgggtagagc aaagacattt
    41461 tttcgcttaa cgacaatata ttcagcaaat ccaccatcac gccgcgagcc aataaaatca
    41521 tattttgcgc actgggaata aaaccctttc agacactctg gacaagtaaa acagggtaat
    41581 aacggcacac aggcaaccgc atcgccaggg tgtaaatcat caacaccgga tcccaccgca
    41641 tcaatatagc cgctaaattc atggcctaac gttattggat aataatgtgc accattttta
    41701 aatatcctgg gtaaatcgga accacataag cccgagctgg caatttttac ccgcacctca
    41761 tcctgatgtt taatttcagg aatgacgctt tctgcaacgc gcacgatacc atcagtatca
    41821 ttcaccactg atttcataaa aactcctgat tgttaagggg gataaactcc ccctcagtta
    41881 aaaattattc tgcgagaacg gctttctctt gtttaataaa accacgcgct ctacgccagg
    41941 taatgaaaat accggtcaga taaattgcgc cgataataat gaaaccggga atattttgcg
    42001 gggagaaaac ctgaatcagt aaccaggtaa tgggagaacc gccctgatcc attgaagcca
    42061 ccataccccc ggcttttaac gccccagcat tagccgccag ttgggtgtgc aaaccaatag
    42121 tttgcgtcgc gatccacaga gtgatgctca tgatgatgac acccgagatt aaggtgcgga
    42181 acagatttcc acgatgcacg gcgactgcca tcgccacaaa gaagccgatg gtggcaagat
    42241 cgccaaacgg cagtacctga ttacccggaa cacaaacagc aattaaaatg gtgagcggga
    42301 taaaaatcag gcttaccgat accaccgccg tatgccccag cagtaatgcg ggatccaagc
    42361 caatcaggaa ctcctgaccg ccgaacttcg cctgtaaacg actacgggcc tgcttagcga
    42421 tgggcgttaa accatccatg atgggtttaa tcacccgtgg cattagcagc atcactgccg
    42481 ccgtttttac cgccagctgc aatacacctt tgacatcgta acccgcgagg atgccgataa
    42541 tcagccccat cacaaaaccc acggtgacag gttcgccaaa agggccaaat ttgcgctgaa
    42601 tatcatcggc gctaaattta attcggttaa cgcctgggat tttctcgatg atagcatcga
    42661 ccagcaccgc aatcggcccc atatacgccg acgtaccgtg cggaatagca ataccttcca
    42721 gctcaaagaa atttcgggta tcgcgggcaa accagtcgcc gagcttataa acaaacgccg
    42781 cgtgaattac cacacccgcc atccctatca tccatgaacc ggttgccaga tgcagcaacg
    42841 cgccggtgaa agtcatatgc cagatattcc agatatcaat atttaccacc cgcgtcatac
    42901 gggtcagtaa catcgccacg ttaaccagaa tagcaatcgg aatcgccacc agcgcaattt
    42961 gcgacgccca ggtcattggt gaagagcccg gccagccgac atcgaccaca tgcagattca
    43021 ggtcgaaatt ttccgccatc gctttcgccg ccggaccaat ggaatccagc attaagccaa
    43081 tcacaaggcc aatgccaaca aagccaatcc ctatatgaag acccgcttta aagcaatcgc
    43141 ctgccttcat gcctaatatt ttagaaaaaa taatgatgac aatcggcagc atcaccgtag
    43201 ggccgaggtc gagaatataa cgcatgactt ctgaaaacat aggtcacccc tgtaagatag
    43261 tcagaatttt attttgtaat gcttcgatac cgacaccaga aacaaaaggc atgccgtgaa
    43321 ctaacggaat atcgccaaaa ctacgatcca ccctggcagt agtgcatatc aaatgcacgc
    43381 catccatata ggtttctatt tcattaaccc gacactggat taattcaaca ggaatattat
    43441 gattctgaca caattcttta atttcttccg ccgccatcgt agaggtcgca accgcgcctc
    43501 cgcaagcgac aataatcttg cgtttcataa atagtccttt ttattgttac agagggattt
    43561 attcgtctga atctaaaaca tactttgtga agtattcctt taactgggtt tcaggaagag
    43621 tgattagtgt ctcgacgata tcgggctgtt gtaacttgcc aaataaacag cgtaaaagtt
    43681 tcaattgctg ctgcggattt tccacaatca acgcaataac caacgatacc tccacgtcgt
    43741 tatcatcatc cgcttgctga aaatgaactt tatttgttgg ccttaacaga taaatggctg
    43801 acgacttagc atgaatcgcc tcacaatgcg gtattgcaat ggcgtgctgc tcaagcatta
    43861 tcccggtagg gaattctgct tctctggcaa ttaatgcctg tggccaggta tcatgaacca
    43921 cacctttggc gagcatctca ttaccgatat gggttaaaac ttcgctacga tcgacaaaag
    43981 aaattccgct acgaacaaac aggttagtca tataccgtcc ttattccgca cagccgtagc
    44041 gataggcgcg caaaacatca taaattttat ccataatcag ctgatgcggt attgctgata
    44101 attccccgga ctgaatgcgt tcaaattgtt tgggaagata ctgactaatc atgcccagtg
    44161 ggatgtccac gccttcaaga ttcaccatca tcgtttcgac gctattttta atccgactat
    44221 gcggccagta ataacgaata cgatccgaca ggctgtaacg aatatccagt aatgaatcgt
    44281 taaaacccgt acgataatat tttttccagt attgcggttc gtcgagcatc acttcttcaa
    44341 ttaccgccag gcaaccgctg cgattttcag gggcgataag ttcctgctca atttgtgcca
    44401 gtgcaaatat cgcctcgcgt aaagcaaagg ttaatgcggg accgactttc aatattgcaa
    44461 agtgatcgcg gactaattcc caataagccg tccgggtctg gtaatcggta gaatgtgctt
    44521 cataaaccat tcgggtgttt tctatccatt gcgccagcgg ctgcgcttcc tgcggctgat
    44581 aatggataat attgctgtga tcaaattcca cgcccggctg caccacgatg gcaatcacgc
    44641 gtgttaacgc ctctgtcagc ccacggacaa taaaggcctt ttgatgcgta cgtaaagtat
    44701 tggcggcatc ttcaacatgg gtgatgtgta ctgactgaat ggcgctggcc tcaccgcccg
    44761 gaaccggaac ttcggtgcca atgacatagc tcagttgctc acgctggcaa tctgtcgcca
    44821 cactttccgc agcaaagcaa agcacagcag ctcgttccgc aaccgtttct ggtgctaacg
    44881 gtatgggatc ccccgcgcag gacattgacg catcaagatg aattttactg aagccggcac
    44941 gaacatattc ctttaccagc tcgacggatt tttccatcgc cgcatccgca ttttcttgct
    45001 gccagcagtt tggccccaga tgatcgccgc cgagaataat gcgttcgcgt gcaaacccaa
    45061 ctttatcggc aatcgtaaaa acaaattcgc gaaagtctgc cggtgtcatt ccggtataac
    45121 cgccaaattg attgacctgg tttgacgttg cttcaatcag cactttgcgc gtgctgttgc
    45181 gatcaaatgc cagcgccgct tcgataacca acggatgggc agaacagact gaacatatgc
    45241 cgatatgttc accagcttta tgccgggcaa ttaacgtttt catgtttttt ccttattaaa
    45301 tggcaggtgc gttatgccct gccctcgcag ccacaatcgg caatcacttt gctcaccaca
    45361 tcgcgcattg cggatttagc cgactgcaaa taatcccggg gctcggtcgc ttcagggtgc
    45421 tcggtcaggt aattttttaa cgcctgcgag aaggcatttt tcagctccgt tgcaacgttg
    45481 attttgcata tccccagttt gatggtttgc tgaatatcct tagtcgataa ccctgacgcg
    45541 ccatgcagca ccagcggtaa gttcacccac tggcgaatgt tctccagtct agaaaaatca
    45601 agcgccggtg cgctggcata catcccatga gccgtgccga tggcgaccgc cagggaatca
    45661 attccggttg cctcggcaaa ttcacgcgcc tgagcggggt tggtataaaa agcatcggct
    45721 tcattgactt gcacatcatc ttcctggccg ccaagttgcc ccagctccgc ttcgacgctg
    45781 acatcaaagc gatggcaaaa atccaccacc tctttgaccc gtgaaatatt ttgcgcaaaa
    45841 ggcaaatgcg aggcgtcaat catgactgag cgcacgccag aacgaacctt ctgagcgata
    45901 tcgtcaaatt tcgtgtgatg gtcgagatga attgccagtg gatggtgata ttgcttcgcc
    45961 atcgcgctga ccagcgccaa cagattttct gtaccagcat gagtaaatgt gccaggcgtt
    46021 ccggcgatga tgaccggcgc atgcaggttg gcagcggttt ccaccaccac ttgcatcgtt
    46081 tcgagattgt gaatattgaa tgccggaacc gcataaccgc cgcgctgtgc gttgttcagc
    46141 atctgctttg tcgataccac gtgcattttc atatcctgtc gtttgttttc gttttcaaaa
    46201 tataatgaaa ttatttgttt ttaaatatcg agataacgat cacaaaaacg acaatatgaa
    46261 aattattcga ggagtgaaag gcaaaaaaac ggcctcccga tagggaagcc gtagcaaagt
    46321 gcgcgtgttt ttatgccgga tgcggtgtaa acatacgtgg cagtgctatg taggcatgat
    46381 aagacgcttc cgcgtcgcat caggcatagg ttgccggatg cagtgtaaac gccttatccg
    46441 tcctacgggg tggtgttgtt cgtataaggc gtatcaggca atagtaattt tgctatcgag
    46501 ataaacgtcc tgcacggcgt taatcagttt cacgccgtca gccatcgatt tcttgaacgc
    46561 cttacgtcca agaatcagcc ccattccgcc agcgcgtttg ttgataaccg cagtacgcac
    46621 tgcatcactg aggtcagttt caccgcctgc agcaccgccg gagtttatca acccagcccg
    46681 gcccatatag cagttagcta actgataacg caccagatca atcgggtttt cgctggttaa
    46741 cttgctgtac acgcgatcgt cggtataacc gtaattaatc gctttatagc cgccgttatt
    46801 ttccgccatt ttttgtttga cgatatctgc gccgatggtt gccgccagat ggtttgcctg
    46861 accggtcagg tcggcggaaa catggtaatc aacgccatct ttcttaaagg cggagttacg
    46921 caaataggcc cacagcacgg tcaccatgcc cagctcatgc gcacgttcaa aagccgcaga
    46981 aatttcttca atctggcgac gtgactcttc cgagccaaaa tagatagtcg caccaaccgc
    47041 caccgcgccc atgttgaaag cctgctccac gctggcatac agcgtttgat cgtaggtatt
    47101 cgggtaactt agcgtctcgt tgtgattaag tttgacgaga aatggaatgc gatgcgcata
    47161 gcgccgcgat accgacgcca acacgccgta agttgatacc acacagttac agcccgcttc
    47221 gatcgccagt tcaacaatgt ttttcgggtc aaagtagagc gggttagcag caaatgaagc
    47281 tccggcagag tgctcaacgc cctggtcaac cggcagaata gaaagatatc ctgtgccagc
    47341 cagacgcccg gtgttgtaca acgtctgcat attacgtaac accgctggcg ggcgattatt
    47401 gtcaatcatt acgcggtcta cgtagtcatg tccggggaga taaagctggt cagaaggaat
    47461 tgtcatacaa cggtgctgta aaaggttgtc ggcgtctttg ccaagcaact gcgcaatatc
    47521 tgtcattact atgctcccgt aaattccgat tggatatcgg ctatggattg tcctggcccg
    47581 ccttttgcgg gcaatcataa tcctggtcgt tacgggcgag tttttccatc ttttggatgt
    47641 tttttcagcg ttttctgttg gctcgattca tcagaaaaaa tgttagcgcg gtcaagtttt
    47701 caccgtaaag gcatttaaaa ggtattatta agtggtattg tcatcgcgta ccttacatta
    47761 cctgtcatga aggaattaaa agatgaaaac aacagcaaag ctgtcgttca tgatgtttgt
    47821 tgaatggttt atctggggcg cgtggtttgt gccattgtgg ttgtggttaa gtaaaagcgg
    47881 ttttagtgcc ggagaaattg gctggtcgta tgcctgtacc gccattgcgg cgatcctgtc
    47941 gccaattctg gttggctcca tcactgaccg ctttttctcg gcgcaaaaag tgctggcggt
    48001 attgatgttc gcaggcgcgc tgctgatgta tttcgctgcg caacagacca cttttgccgg
    48061 gttctttccg ttactgctgg cttactctct aacctatatg ccgaccattg cgctgactaa
    48121 cagcatcgct tttgccaacg tgccggatgt ggagcgtgat ttcccgcgca ttcgtgtgat
    48181 gggcactatc ggctggattg cctccggtct ggcatgtggt ttcttgccgc aaatactggg
    48241 gtatgccgat atctcaccga ctaacatccc gctgctgatt accgccggaa gttctgctct
    48301 gctcggtgtg tttgcgtttt tcctgcccgg cacgccacca aaaagcaccg gcaaaatgga
    48361 tattaaagtc atgctcggcc tggatgcgct gatcctgctg cgcgataaaa acttcctcgt
    48421 ctttttcttc tgttcattcc tgtttgcgat gccactggcg ttctattaca tctttgccaa
    48481 cggttatctg accgaagtcg gcatgaaaaa tgccaccggc tggatgacgc tcggccagtt
    48541 ctctgaaata ttctttatgc tggcattacc gtttttcact aaacgctttg gtatcaaaaa
    48601 ggtattattg cttggtctgg tcaccgctgc gatccgctat ggcttcttta tttacggtag
    48661 tgcggatgaa tatttcacct acgcgttact gttcctcggt attttgcttc acggcgtaag
    48721 ttacgatttt tactacgtta ccgcttacat ctatgtcgat aaaaaagccc ccgtgcatat
    48781 gcgtaccgcc gcgcaggggc tgatcacgct ctgctgccag ggcttcggca gtttgctcgg
    48841 ctatcgtctt ggcggtgtga tgatggaaaa gatgttcgct tatcaggaac cggtaaacgg
    48901 actgactttc aactggtccg ggatgtggac ttttggcgcg gtgatgattg ccattatcgc
    48961 cgtgctgttc atgatttttt tctgcgaatc cgacaacgaa attacggcta tcaaggtcga
    49021 tgatcgcgat attgcgttga cacaagggga agttaaatga aaacagaacg tattctcggt
    49081 gctctttatg ggcaggcgtt aggggatgcg atggggatgc cctccgagct ttggccacgc
    49141 agccgcgtta aagcacactt tggctggatt gaccgttttc tgcctggacc aaaggagaat
    49201 aacgcggcct gttattttaa ccgcgccgaa ttcaccgacg atacctcgat ggcgctgtgt
    49261 ctggcggatg cgttgctgga acgtaaaggc aagatcgatc cggatctgat tgggcgtaat
    49321 atcctcgact gggcgctgcg tttcgacgcc ttcaacaaaa acgtgctagg tccgacctcg
    49381 aagattgcgc ttaacgccat tcgcgacggt aaacccgttg ctgaactgga aaacaacggc
    49441 gtaaccaacg gcgcagcgat gcgcgtctcg ccattaggtt gtttgcttcc ggcgcgcgat
    49501 gttgattcct ttattgatga tgtggcgctg gcgtcgagcc cgacccataa atccgatctg
    49561 gcagttgcag gtgcggtagt catcgcatgg gcgatttctc gtgctattga cggagaaagc
    49621 tggtcagcga ttgttgattc actgccttca attacgcgac atgcccagca aaaacgcacc
    49681 accacgttca gcgcctcact ggcggcgcgg ctggagattg cgctgaaaat tgtgcgcaat
    49741 gccgacggca ccgaatccgc cagcgaacag ctttaccagg tcgttggcgc aggtaccagc
    49801 actattgagt ccgttccgtg cgccattgcg ctggttgaac tggcacaaac cgacccgaac
    49861 cgttgcgccg tcctctgcgc taaccttggc ggcgatacag acaccatcgg tgctatggcg
    49921 acggcaatct gcggcgcgtt gcatggcatt aacgctatcg atcctgcgtt aaaggcggag
    49981 ctggatgcgg taaatcagct tgatttcaac cgctatgcca cagcgctggc gaaatatcgt
    50041 caacaacggg aggcggtatg agcggcgctc gattacacac gctgctgcct gaattaacca
    50101 cgcgtcagtc ggtgatggtc gtcggcgcgg cggtcattga cgtgatcgcc gacgcttatg
    50161 ccctcccctg gcgtgggtgc gatatcgaac tgaaacagca gagcgttaac gttggcggct
    50221 gcgcactgaa tattgccgtg gcgttaaagc gcctcggcat cgaagcgggt aatgccttgc
    50281 cgctcggtca gggggtatgg gcggagatta ttcgcaaccg gatggcgaaa gaggggttaa
    50341 tcagcctgat cgataacgcc gaaggcgata acggctggtg tctggcgctg gttgagccgg
    50401 atggcgaacg tacttttatg tcattcagcg gtgtggaaaa tcagtggaat cgccagtggc
    50461 tggcgcgatt aaccgttgcg cctggcagcc tgctctattt ttccggttat caactggcct
    50521 cgccctgcgg cgaattgtta gtggaatggc tggaagagct gcaagacgtg acgccgttta
    50581 tcgattttgg cccacgtatt ggcgatatcc cggacgcatt actggcgcgg atcatggcct
    50641 gtcgaccttt agtgtcactc aatcgtcaag aggctgagat tgccgccgaa cgttttgctt
    50701 tatccgcaga gataacaaca cttggcaagc aatggcagga gaaatttgcc gcgccgttga
    50761 tcgttcgtct cgataaagaa ggcgcatggt atttcagcaa cgacgcttct ggctgcattc
    50821 cggcatttcc aacgcaagtt gtcgacacca ttggggcggg cgacagtcat gccggtggcg
    50881 tacttgccgg gctggcctct ggtctgccac tggcggatgc cgtattactg ggcaatgcag
    50941 tggcgtcatg ggttgtcggg catcggggcg gtgattgtgc gccaacgcgc gaggaactac
    51001 tcctcgcaca caaaaacgta tagatcgctg cgacagtggc taatgctgta ctcaataggc
    51061 cgttgctgtt ggtcaagcgc cacttgcttg atcaccagca ccggtatttt gctatctaac
    51121 tgaatatgtg actgaaactc ggcatccggc atccgggcgc taacgcggga acgcgtacgt
    51181 tgtgggtaaa tgtgctggct gcggaaatag tcataaagtg aaatcccgat ggcatcaaca
    51241 tcatgaatta aatgcgccgg aacccacgat tcctcaatcg ataccgcttc ttcatcgaca
    51301 tagcgaatac gcttaagcaa aaagacatca cttcctgctt cgaccgccag ctgctgcgcg
    51361 acttcttcgg gacatttcac gacacgcttg ttaacccata gcgtatcggg ctttttcccg
    51421 cgcaatacca cctgctgaga aaaacctcgc gcttctttca acgagtattc gaagatgttg
    51481 ttgatctgcg tgccgtaacc gcgcgaacgc gtcaccacac cctcttcttc cagcgcctgc
    51541 atcgccttgc gcaccgtaat gcgcgaaacg ccggttaact gacttaaatc acgctcaccg
    51601 ggcaaaatat tgccatgctc cagcaccccg ctgcgcacgg cattttttac cgtttcggca
    51661 aacttaatat aaagcggcgt gttatccgcc gctaaaatac gctcgttcag ttgggcaatc
    51721 agctgggtat gcgcttgttc catttgtctt gttcctgccg gggagtctgg tgggagtata
    51781 tcactgcttc ttatttaagg cgtttcttta atcccatcaa cgaatgcctg caactcctcc
    51841 accgtgccat taaatacatt aaaatccacc ggaccattga tgccatctac ctgtccacgg
    51901 tcggaatgct gccagaagcg ccaggccatg ccgtcattgt ccggacgacg ttgataatag
    51961 tgagccaccc accacggata ttcattgaaa tagcccgcca gattggtgtg ataaaaaacg
    52021 gctcctgagt aaataatcgg cttttttccc gtactttttt cgaccatttt tagccactga
    52081 cttactcgtt tgcgtaattc ttttgccgat aattttcccc gttcttctac gtccagtacc
    52141 gcagggaaat caccttgtga gaaatccacc gtttgcagaa ataatcgtgc ctgaaccggc
    52201 gctgctaccg acggggagaa ataatgatac gccccgcgca gcaggccatt ttcgcggctt
    52261 agttgccagt tacgcgaaaa atacgggtcc accagctttt cgccttccgt cgccttaata
    52321 aaagcaaact gtaagcggat accgttgtcg cgcatttttg ccacccgctg ccagtcgatc
    52381 cgctcctgcc agcgagaaac atctattccg tgaatggtgt aactggcagg aatgcgaata
    52441 gcgaaagatt taaccgggcg ataaccgtag aaatggatgt agtctgtaat ctgaactgct
    52501 gtcgaataga aaaaattcac cttctgacgc ggataaattg caacgatgga aattagccca
    52561 agagcgcata atagagaggt gagttttttt cgactggtaa ttcttagctg cattggtttc
    52621 atccctgaat gtcagtgcca aaggctgaca ataaccaaag cagctatagt acggtgctca
    52681 ccaccaggcg tggaagtggt gaaccggacc aataccgtga ccaacttcca gcgtgtcggc
    52741 ctgggctaat gctgatgaaa gccagctttt tgcctcctgt acggtgtcag cccagtttgt
    52801 atggcgcggg cgtagtgcag ccaacgccgc agagagcgta caaccagtgc cgtgggtgtt
    52861 tttggtcata atgcgcggtg cggtaaaccg ttgttcaccc tcgcgggtaa acagccagtc
    52921 cgggctttgc tcatcatcca gatgaccacc tttcattagc actgcgccac agcccatcgc
    52981 caacagcgat cgcccttgtt ccagcatttc ctgttcagtg cgcgcgtgtg gcgcgtcgag
    53041 caaggcggca gcttcgggca agtttggcgt tattaatgaa acctgtggca ataatcgact
    53101 gcgcagcgta gcaaccgccg aaggtgaaag cagcgggtcg ccgctttttg ccagcataac
    53161 ggtgtcgagt accacgtatt ggatctgata acgttgcaac cgttctgcca ccgcttcaac
    53221 aatatcggtt tccgccaaca taccgatttt agtggtgtcg attcgcacat cgctgaacac
    53281 cgaatcgagc tgggcggcga caaaatcaga ctcaatgcga tacaccgact gtacgccacg
    53341 ggtattttgc gccaccagtg cagtaataac tgagcaacca taagcgccaa gtgccgagaa
    53401 ggttttaaga tcggcctgaa tccccgcacc accactcgga tcagtaccgg caatcgtcag
    53461 agcgttaatt cgtttcatgc ctgcacctcc tgcgtcaatt gccagagcgc atcaaggaaa
    53521 tgtggaacaa aactgcctgg cccctcgctt ctggcgactg cgcgttctcc ggcttgtttc
    53581 atccagtgac aggcagatgc gacattttcc agcgtatcgc ctggtaacgc acagcaggca
    53641 gcgacaaccg ccgataatgc acagccagtt cctaccactt tggtcattaa cggatcgcca
    53701 ccgtgaatac caatgatacg atgtccatcg gtaacataat ccatctcgcc agtgaccacg
    53761 acgattgcgc cagtttcccg tgccagtgtt tgtgcagcgg gtatcgcgtt agctgcggcg
    53821 tcagtggtat ccactccccg tccaccatta gcaatgccag ctaatgccat gatttccgaa
    53881 gcattaccac gtatagcggt tggcttatga ggcagaagtt ccagacaaaa acggcggcga
    53941 taatcgagtg caccaaccgc tactggatca agcgtccagg gtgtttgaga gctttttgct
    54001 tgctcaacgg cggcacacat cgactgtgcg cgtagctgcg tcagcgtgcc aacgttaatc
    54061 agcaacgcac tagcaatagc cgcaaactga ctggcctctt cggtttcgat aaccatcgct
    54121 ggcgatgcac cgagcgccag caaggtattg gcagtaaagg tttgcaccac atcgttggtc
    54181 atgcagtgca caagagggga atgttggtga aaaaggtgta acgcgtgcgc agattgcgct
    54241 gaactctgca ggtcgacttg catagtttgc tcctgccata acgtgaagaa gcaatgacct
    54301 ggtggtccgt gacttcccta cgctggcatt atccagatca ggtaatacgg gtatttctca
    54361 gccttcacaa agaagggcac cccgagtcat ttaaaacaaa atcaaaagat tgcgattaac
    54421 gtcccgttaa tccctgttct ggatcatgcc agcggaaagc caaagactca accctaaaaa
    54481 atcctctgtg tcccagtttg tgtctcacgt tgtggcccac agataaatgg caggcacaaa
    54541 aaaaccgccc ataaggcggc ttaagtaaat tattcagtcg tgaagattta aatgaaatct
    54601 attttctgca tacgctttct tcatgcagtc gaagtgcttt tgccaattcc aattggaatg
    54661 caaaggaaag acgggaacgg ctgatcttga taccgtcaat aatgatgtat gtagggtaaa
    54721 cagtgaccat gcggcccaag tattggaagt gtttagggtg gtttacttta tcgttattca
    54781 ttgtgtgcca tccgtctggg tgggggtaat cccggcaacg gatgattgat ctaaaatcga
    54841 tcaaaatcaa taacttaaca gatcaccaaa tccagggggg tgtacattgg cgagcctccc
    54901 tcagcctaac ctatacacga ccataaaata ggggcggatt ccttccagtt gggtgggaaa
    54961 atatctggga gtttggatac agtagccagg cagggtaacc agacccatag atgagaatgt
    55021 ttattattcc caaatatcca ggcgaggggc ctgctgtggc tctctttacg cgccgctgtg
    55081 gcgtcttctc tcctctttct atacaatccc atagctaacc cattgcgagg cttacagagc
    55141 gttacagagc gtccgtgtga atattgcata aatgaaagtg gtgaaaggtc cgatttggtc
    55201 aacttttaga caactattta acatatgcgc aacaggttag acctgtaccc tttcaatagg
    55261 tccgcctcct gggcgctgtg tacgccccat aactgtggct cttctgtacc catccgggag
    55321 ccattccaca gagtaaactg taaaactgat ctgcctgtga gtaacgccct tttcctgtgg
    55381 ataacttcca cgcactaacc ccataaactc cccttttgag gcgtccgcct cttcaggccc
    55441 tttcttgtgg caaaaacaca aaaagggttt agggcggtgg ctaccgcccc tgtagatggg
    55501 aatgagtcgc actcgaaaga gggattgaga accaattctc aagtgataat cgatctcatt
    55561 tgtgttcgaa gttttcccca gaaaaataat tttggggtta agcatgtaaa atgctacaca
    55621 cttgcacttt gtaatgataa ttaattgacc ataaaaacaa atagttagaa gcgctggggg
    55681 agtcccacta gttacacaat tgattgcttt ttgagctaaa actgattctt ttaccgggta
    55741 tacaatccat ttgatcgccc tttaccacat atttaaacgg atttaaagcc atttatttag
    55801 ggggttagca aagtgttgat tccatggtta cagataacaa aaaagcccgc cgggggttag
    55861 ccgggggcct gtggttgggg gtgtcaaacg tggttagttc ggcccgccct gcattagcat
    55921 gttgaaagcc tgctgattat tgctctcaac ttcagagcgt accagatcct taacctatcc
    55981 atcctgcact ttcacattaa cgttcacgtt tagcggctgg tttagccacg ctggcggcgt
    56041 catcagtgga ttacctgcca tatcaacttt aagctgtggc gctgtaatcg tcggcgtagg
    56101 aggtacataa gttggattgg agtaagagct tacccaccct gttttatctg ctggtttggc
    56161 aaagtctgga ttagtcggaa ggtctggact atggaggaaa tcccacagtt tacccagcgg
    56221 gctatctttt ttcagattgc ccccggcggt ggtgtcctgt ccatacatgg cgtcgttagc
    56281 gtcatgacct tatcccaggc ataatcccca cctaccgaac cgaccgcaat gctccagcca
    56341 acaggaccgc cagagagagc cgccgcgccg cctaccttgc caactttcgg accccgaccc
    56401 ggtttgtttg gcttgtcttt accttcccca ccagatccgc cagtagtagc ggcttcaatg
    56461 ccaccaatag cggcgatccc tttcatgacc cccagtaatt tggttagtgg gttaagcatg
    56521 gtcaacaacc atttaaaggc tacgcccaac ttccagatag acccggcgaa catagccacc
    56581 ccggcggcgt atgctgccat ttcgccccag gctttaagct gctcaccagt gatccccatc
    56641 ttgctggcgt aatactcaat aatgcggaag gtcaggatca cagtgtcatg caactctgta
    56701 aacgcatctg tggcaccctc aacgattttc ccggcaatat gcccgaacat ctcaaaggcc
    56761 ccaccagagc cgttaacagc ggcgctgata gcgtcgaaag cgctggttaa ttcgttgccg
    56821 aagccagaag caaagaagat gttcatcgcg ttctgcatac tggtttgcag gcgctgccag
    56881 cttgcccggt tggacttcat agccttatcc agagcgccgc cgttacgagc tgcggctttc
    56941 atctgtttag ccacttccgg gaggatgtcc ttagagaaca gcttcccttg ttgcatcagg
    57001 tcaaacagat ccttttctgt aaggttggtt gtccctttca tctggttgag agccttaacg
    57061 aacagaccca cagcccccgg gagagcttcg gacaactggc ctttgagttc ttcactcatt
    57121 accgttcctt tagacatcat ctgattcaaa cttttcaaag cgcggctggc ttcatcggct
    57181 gaagcacctg taacaaggcc ccattcactg gcaccctcaa agatggatcg catctggtct
    57241 ttagggagct tgtcaccaac agcggcggca aactgagtgt aatcacggga agtcttgagg
    57301 agatccagcc ccaaacggtt ggattgatca cggagataat caaactcttt agcggcctta
    57361 tctgtggaac cggtgatcgc tgtcagacca gaaagcgcgg attcaaaatc ctgtccggtg
    57421 gtcatgatct tgccgctcag cgccacagtt gcggcaatgg tgatcgcccc gcctgcaccc
    57481 tcgatcatgg
//
LOCUS       DAJTMC010000006        52005 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.006, whole genome shotgun sequence.
ACCESSION   DAJTMC010000006 DAJTMC010000000
VERSION     DAJTMC010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 52005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52005
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.006"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            <1..493
                     /locus_tag="OO130_000344"
     CDS             <1..493
                     /locus_tag="OO130_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HCR5363879.1"
                     /translation="NQLDSHDTARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEV
                     GLDGKNDPFCRKPFPWQVEKQDTALFALYRRMIVLRKKSQALRRGGCQVLYAEDNVVV
                     FVRVLNQQRVLVAINRGEACEVVLPASPFLNAVQWQCKEGHGQLTDGILALPAISATV
                     WMN"
     gene            complement(498..1079)
                     /gene="acpH"
                     /locus_tag="OO130_000345"
     CDS             complement(498..1079)
                     /gene="acpH"
                     /locus_tag="OO130_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HCR5363880.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRRETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFI
                     CYARKQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLNAHYTALGTRFWQFYPRMMAQASHKAL"
     gene            1172..2242
                     /gene="queA"
                     /locus_tag="OO130_000346"
     CDS             1172..2242
                     /gene="queA"
                     /locus_tag="OO130_000346"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HCR5363881.1"
                     /translation="MRVTDFFFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTSVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDDRSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            2298..3425
                     /gene="tgt"
                     /locus_tag="OO130_000347"
     CDS             2298..3425
                     /gene="tgt"
                     /locus_tag="OO130_000347"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HCR5363882.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            3448..3780
                     /gene="yajC"
                     /locus_tag="OO130_000348"
     CDS             3448..3780
                     /gene="yajC"
                     /locus_tag="OO130_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HCR5363883.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            3808..5655
                     /gene="secD"
                     /locus_tag="OO130_000349"
     CDS             3808..5655
                     /gene="secD"
                     /locus_tag="OO130_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HCR5363884.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            5666..6637
                     /gene="secF"
                     /locus_tag="OO130_000350"
     CDS             5666..6637
                     /gene="secF"
                     /locus_tag="OO130_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HCR5363885.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGEDLTQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            6766..7113
                     /locus_tag="OO130_000351"
     CDS             6766..7113
                     /locus_tag="OO130_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HCR5363886.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(7290..8174)
                     /gene="tsx"
                     /locus_tag="OO130_000352"
     CDS             complement(7290..8174)
                     /gene="tsx"
                     /locus_tag="OO130_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HCR5363887.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLNFGPFKEWYFANNYIYDMGRNKNGRQSTWYMGLGTDIDTDLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(8473..9012)
                     /locus_tag="OO130_000353"
     CDS             complement(8473..9012)
                     /locus_tag="OO130_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HCR5363888.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGGYLLPRAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            9163..9612
                     /gene="nrdR"
                     /locus_tag="OO130_000354"
     CDS             9163..9612
                     /gene="nrdR"
                     /locus_tag="OO130_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HCR5363889.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            9616..10719
                     /gene="ribD"
                     /locus_tag="OO130_000355"
     CDS             9616..10719
                     /gene="ribD"
                     /locus_tag="OO130_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HCR5363890.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVASMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRLRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHHIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWGEAGPTLAGALLQAGLVDELIVYIAPKLLGSDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            10808..11278
                     /gene="ribE"
                     /locus_tag="OO130_000356"
     CDS             10808..11278
                     /gene="ribE"
                     /locus_tag="OO130_000356"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HCR5363891.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            11298..11717
                     /gene="nusB"
                     /locus_tag="OO130_000357"
     CDS             11298..11717
                     /gene="nusB"
                     /locus_tag="OO130_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HCR5363892.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            11795..12772
                     /gene="thiL"
                     /locus_tag="OO130_000358"
     CDS             11795..12772
                     /gene="thiL"
                     /locus_tag="OO130_000358"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HCR5363893.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGVRIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTLDWKGYDHFATP"
     gene            12750..13268
                     /gene="pgpA"
                     /locus_tag="OO130_000359"
     CDS             12750..13268
                     /gene="pgpA"
                     /locus_tag="OO130_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HCR5363894.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(13322..14296)
                     /gene="yajO"
                     /locus_tag="OO130_000360"
     CDS             complement(13322..14296)
                     /gene="yajO"
                     /locus_tag="OO130_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HCR5363895.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHGWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHPVVGFK"
     gene            complement(14351..16213)
                     /gene="dxs"
                     /locus_tag="OO130_000361"
     CDS             complement(14351..16213)
                     /gene="dxs"
                     /locus_tag="OO130_000361"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HCR5363896.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPEAAKVAESLNATLVDMRFVKPLDETLILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            complement(16238..17137)
                     /gene="ispA"
                     /locus_tag="OO130_000362"
     CDS             complement(16238..17137)
                     /gene="ispA"
                     /locus_tag="OO130_000362"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HCR5363897.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDADMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSPDTSALEALADYIIQRNK"
     gene            complement(17137..17379)
                     /gene="xseB"
                     /locus_tag="OO130_000363"
     CDS             complement(17137..17379)
                     /gene="xseB"
                     /locus_tag="OO130_000363"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HCR5363898.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            17585..19033
                     /gene="thiI"
                     /locus_tag="OO130_000364"
     CDS             17585..19033
                     /gene="thiI"
                     /locus_tag="OO130_000364"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HCR5363899.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPEVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIALQTE
                     QEVVEVETVNGFGPNDVILDIRSIDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNR
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            complement(19087..19677)
                     /gene="yajL"
                     /locus_tag="OO130_000365"
     CDS             complement(19087..19677)
                     /gene="yajL"
                     /locus_tag="OO130_000365"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HCR5363900.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLQVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(19640..20551)
                     /gene="panE"
                     /locus_tag="OO130_000366"
     CDS             complement(19640..20551)
                     /gene="panE"
                     /locus_tag="OO130_000366"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HCR5363901.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSANLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            20719..21210
                     /gene="yajQ"
                     /locus_tag="OO130_000367"
     CDS             20719..21210
                     /gene="yajQ"
                     /locus_tag="OO130_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HCR5363902.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            complement(21338..22702)
                     /locus_tag="OO130_000368"
     CDS             complement(21338..22702)
                     /locus_tag="OO130_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5363903.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHILLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPA
                     YVSSLRIEIPANIAANEALKVRLLETEGIKEVLIAEEEHSAYVKIDSKVTNRFDVEQA
                     IRQA"
     gene            complement(22851..23741)
                     /gene="cyoE"
                     /locus_tag="OO130_000369"
     CDS             complement(22851..23741)
                     /gene="cyoE"
                     /locus_tag="OO130_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HCR5363904.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            complement(23753..24082)
                     /locus_tag="OO130_000370"
     CDS             complement(23753..24082)
                     /locus_tag="OO130_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HCR5363905.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIITILVVGSIWIMWNL
                     NYNMMMH"
     gene            complement(24082..24696)
                     /locus_tag="OO130_000371"
     CDS             complement(24082..24696)
                     /locus_tag="OO130_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HCR5363906.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTCLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            complement(24686..26677)
                     /gene="cyoB"
                     /locus_tag="OO130_000372"
     CDS             complement(24686..26677)
                     /gene="cyoB"
                     /locus_tag="OO130_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HCR5363907.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWANSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            complement(26699..27646)
                     /gene="cyoA"
                     /locus_tag="OO130_000373"
     CDS             complement(26699..27646)
                     /gene="cyoA"
                     /locus_tag="OO130_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HCR5363908.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
     gene            complement(28107..29582)
                     /gene="ampG"
                     /locus_tag="OO130_000374"
     CDS             complement(28107..29582)
                     /gene="ampG"
                     /locus_tag="OO130_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HCR5363909.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            complement(29626..30204)
                     /locus_tag="OO130_000375"
     CDS             complement(29626..30204)
                     /locus_tag="OO130_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5363910.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLAASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKITKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            30509..30826
                     /gene="bolA"
                     /locus_tag="OO130_000376"
     CDS             30509..30826
                     /gene="bolA"
                     /locus_tag="OO130_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HCR5363911.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            31170..32468
                     /gene="tig"
                     /locus_tag="OO130_000377"
     CDS             31170..32468
                     /gene="tig"
                     /locus_tag="OO130_000377"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HCR5363912.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDSAVEA
                     EDRVTIDFTGSVDSEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            32713..33336
                     /gene="clpP"
                     /locus_tag="OO130_000378"
     CDS             32713..33336
                     /gene="clpP"
                     /locus_tag="OO130_000378"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HCR5363913.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            33462..34736
                     /gene="clpX"
                     /locus_tag="OO130_000379"
     CDS             33462..34736
                     /gene="clpX"
                     /locus_tag="OO130_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HCR5363914.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            34924..37278
                     /gene="lon"
                     /locus_tag="OO130_000380"
     CDS             34924..37278
                     /gene="lon"
                     /locus_tag="OO130_000380"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HCR5363915.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENCVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            37487..37759
                     /gene="hupB"
                     /locus_tag="OO130_000381"
     CDS             37487..37759
                     /gene="hupB"
                     /locus_tag="OO130_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HCR5363916.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            37951..39822
                     /gene="ppiD"
                     /locus_tag="OO130_000382"
     CDS             37951..39822
                     /gene="ppiD"
                     /locus_tag="OO130_000382"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR5363917.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            39973..40344
                     /locus_tag="OO130_000383"
     CDS             39973..40344
                     /locus_tag="OO130_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HCR5363918.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            40438..40836
                     /gene="fadM"
                     /locus_tag="OO130_000384"
     CDS             40438..40836
                     /gene="fadM"
                     /locus_tag="OO130_000384"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HCR5363919.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(40888..41583)
                     /gene="queC"
                     /locus_tag="OO130_000385"
     CDS             complement(40888..41583)
                     /gene="queC"
                     /locus_tag="OO130_000385"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HCR5363920.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMEKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(41648..43348)
                     /locus_tag="OO130_000386"
     CDS             complement(41648..43348)
                     /locus_tag="OO130_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HCR5363921.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEQALETGKQQDVLELAQLALG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHWETSTDGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPHWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            43448..44230
                     /gene="cof"
                     /locus_tag="OO130_000387"
                     /pseudo
     CDS             43448..44230
                     /gene="cof"
                     /locus_tag="OO130_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
     gene            44419..44877
                     /gene="decR"
                     /locus_tag="OO130_000388"
     CDS             44419..44877
                     /gene="decR"
                     /locus_tag="OO130_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HCR5363922.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            44907..46679
                     /locus_tag="OO130_000389"
     CDS             44907..46679
                     /locus_tag="OO130_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCR5363923.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGQILMWIATMVLIAVVVYFLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSISMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASENIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDAPEIREEAVDA"
     gene            46672..48453
                     /locus_tag="OO130_000390"
     CDS             46672..48453
                     /locus_tag="OO130_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCR5363924.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKMVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDR
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            48634..48972
                     /gene="glnK"
                     /locus_tag="OO130_000391"
     CDS             48634..48972
                     /gene="glnK"
                     /locus_tag="OO130_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HCR5363925.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            49002..50288
                     /gene="amtB"
                     /locus_tag="OO130_000392"
     CDS             49002..50288
                     /gene="amtB"
                     /locus_tag="OO130_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HCR5363926.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLMQVTVTFALVCILWVVYGYSLAFGEGNNFFGNVNW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQELEGLDVNSHGENAYNA"
     gene            complement(50337..51197)
                     /gene="tesB"
                     /locus_tag="OO130_000393"
     CDS             complement(50337..51197)
                     /gene="tesB"
                     /locus_tag="OO130_000393"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HCR5363927.1"
                     /translation="MSQALKNLLALLNLEKIEAGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            51415..51987
                     /locus_tag="OO130_000394"
     CDS             51415..51987
                     /locus_tag="OO130_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5363928.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINKGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
BASE COUNT        12755 a        12934 c        14026 g        12290 t
ORIGIN      
        1 taatcagctc gacagccacg atactgcgcg atttaaaacg ctgctcggtc gggatattgc
       61 gcgcctgccg ctggcggtgg tctggctgtt cacctggcct ggtgtaccgt gcatttatta
      121 cggtgatgaa gtgggactgg atggtaaaaa cgatccgttt tgccgtaaac cgttcccctg
      181 gcaggtggaa aagcaggata cggcgttatt cgcgctgtac cggcgaatga ttgtgctgcg
      241 taagaaaagt caggcgctgc gtcgtggcgg ctgtcaggtg ctgtatgcgg aagataacgt
      301 agtggtattt gtccgcgtgc tgaatcagca acgtgtactg gtggcaatca accgtggcga
      361 ggcctgtgaa gtggtgctac ccgcgtcacc gtttctcaat gccgtgcaat ggcaatgcaa
      421 agaagggcat gggcaactga ctgacgggat tctggctttg cctgccattt cggctacggt
      481 atggatgaac taacgtttta taacgccttg tgtgacgcct gcgccatcat ccgcggataa
      541 aactgccaga agcgggttcc gagggcggta taatgggcgt ttaaatcgta ccaggagtca
      601 cgcagggcat ccagacgtgg gcgacggctt gccatgccgt ttaacacgtt ctggatgaaa
      661 tccatatcgc gatagcgcac cagccactgc tctgaccaca agtaattgtt tagattgata
      721 aaacgtggcg gtgagtccgg caaaatcgtc atcacttgct tgcgggcata acagataaat
      781 tcctgtagcg gaaagtccgg cgacagctgc gaccagtggc gggaaagaaa gtgatcccac
      841 atgacatcca gcgtaatagg cgcaacgcgg cgcgtttcac gacgaaacca ctcccgtgct
      901 tcgcggactt ccggcagatt gtcagtcaat acgtcgatac gtcgatgcat atgaatgcca
      961 gccacgacgt cgggcggaaa actttcttca ggatttccgc gtacgaaatc agccagtaaa
     1021 ttgccggaaa gcgagctttc cgcgagatgg gctaaatgca ggtgagctaa aaaattcatc
     1081 gattatattc tatccaaagg ggggtaaagg ttgcagggag agcgccccgg cactagacta
     1141 cccgcctctt attttagtct gagtcagtgt catgcgcgtt accgatttct tctttgaatt
     1201 gcccgaatcc ctgattgccc actaccccat gcctgaacgc agtagctgtc gtttactgtc
     1261 gctggacggg cctacgggcg cgctgacgca cggtactttc accgatttac tcgataagct
     1321 caaccccggc gatcttctgg tttttaataa tactagcgtg atcccggcgc gcctgtttgg
     1381 gcgtaaagcc agcggcggca agattgaagt gctggttgag cggatgctcg acgacaaacg
     1441 cattcttgcg catattcgcg cctcgaaagc gccaaaacct ggcgcagaac tgctgctggg
     1501 cgatgacgaa agtattaacg caacaatgac cgcgcgccac ggcgcactgt ttgaagtcga
     1561 attcaatgat gatcgttcgg tactggatat cctcaacagt atcgggcata tgccgctgcc
     1621 gccgtatatc gaccgtccgg acgaagacgc tgaccgcgaa ctttatcaaa ccgtttatag
     1681 cgaaaaaccg ggcgcggttg cagccccgac cgctggtctg cattttgacg agcctttgct
     1741 ggaaaaattg cgcgccaaag gcgtggagat ggcgtttgtg acgttgcacg ttggtgcggg
     1801 caccttccag ccggtgcgcg tcgacaccat tgaagatcac atcatgcact cggaatacgc
     1861 tgaagtaccg caggatgtgg tagacgcggt actggcggcg aaagcgcgcg gtaaccgggt
     1921 gattgcggtt ggcaccactt cagtacgttc gctggaaagc gcggctcagg cagcgaaaaa
     1981 cgatctcatt gaaccgttct tcgacgatac ccaaatcttt atctatccgg gcttccagta
     2041 caaagtggtc gatgcgctgg tgacgaactt ccacttgcca gagtcgacgc tgattatgct
     2101 ggtttcggcc tttgccggtt atcaacacac catgaacgcc tataaagcag cggtagaaga
     2161 gaaatatcgc ttttttagtt acggtgatgc gatgtttatt acgtacaatc cgcaggcaat
     2221 taatgagcgc gtcggggagt aattccgcgg cgctggttta aaacgttgga ctgtttttct
     2281 gacgtagtgg agaaaaaatg aaatttgaac tggacaccac cgacggtcgc gcacgccgtg
     2341 gccgcctggt ctttgatcgt ggcgtagtgg aaacgccttg ttttatgcct gttggcacct
     2401 acggcaccgt aaaagggatg acgccggaag aagttgaagc cactggcgcg caaattatcc
     2461 tcggcaacac cttccacctg tggctgcgcc cgggccagga aatcatgaaa ctgcacggcg
     2521 atctgcacga ttttatgcag tggaaggggc cgatcctcac cgactccggg ggcttccagg
     2581 tcttcagcct tggcgatatt cgtaaaatca ccgaacaggg cgtgcacttc cgtaacccga
     2641 tcaacggcga tccgattttc ctcgatcctg aaaaatcaat ggagattcag tacgatcttg
     2701 gttcggatat cgtcatgatc tttgatgagt gtacgccgta tcctgctgac tgggattacg
     2761 caaaacgctc catggagatg tctctgcgtt gggcgaagcg tagccgtgag cgttttgaca
     2821 gtctcggaaa caaaaatgcg ctgtttggta tcattcaggg cagcgtttac gaagatttac
     2881 gtgatatttc tgttaaaggt ctggtagata tcggttttga tggctacgct gtcggcggtc
     2941 tggctgtggg tgagccgaaa gcagatatgc accgcattct ggagcatgta tgcccgcaaa
     3001 ttccggcaga caaaccgcgt tacctgatgg gcgttggtaa accagaagac ctggttgaag
     3061 gcgtacgtcg tggtatcgat atgtttgact gcgtaatgcc aacccgcaac gcccgaaatg
     3121 gtcatttgtt cgtgaccgat ggcgtggtga aaatccgcaa tgcgaagtat aagagcgata
     3181 ctggcccact cgatcctgag tgtgattgct acacctgtcg caattattca cgcgcttact
     3241 tgcatcatct tgaccgttgc aacgaaatat taggcgcgcg actcaacacc attcataacc
     3301 ttcgttacta ccagcgtttg atggcgggtt tacgcaaggc tattgaagag ggtaaattag
     3361 agagcttcgt aactgatttt taccagcgtc aggggcgaga agtaccacct ttgaacgttg
     3421 attaatatta ataatgaggg aaatttaatg agctttttta tttctgatgc ggtagcggca
     3481 acgggtgcac cggcgcaagg tagcccgatg tctttgattt tgatgctggt ggtattcggt
     3541 ctgattttct atttcatgat cctgcgtcca cagcagaagc gcaccaaaga acacaaaaag
     3601 ctgatggact ccattgccaa aggtgatgaa gttctgacga acggtggcct ggttggtcgc
     3661 gtaaccaaag tagcggaaaa cggctacatt gctatcgcgc tgaatgacac cactgaagta
     3721 gttattaaac gtgacttcgt agctgccgtc ctgccgaaag gcaccatgaa ggcgctgtaa
     3781 ttaaaatttt tccctaaggg aattgccgtg ttaaaccgtt atcctttgtg gaagtacgtc
     3841 atgctgatcg tggtgattgt catcggtctg ctgtatgcgc ttcccaacct gtttggtgag
     3901 gatccggctg ttcagatcac tggtgcgcgc ggagtcgccg ccagtgagca aacgctgatc
     3961 caggtccaga aaacgttaca agaagaaaaa ataactgcta agtctgtggc actggaagag
     4021 ggcgctattc ttgcgcgctt cgactccact gacacccagc tgcgcgctcg tgaagcatta
     4081 atgggcgtta tgggtgacaa atacgtcgtg gcgcttaacc ttgccccggc aacgccgcgc
     4141 tggctggcag ctattcacgc tgagccgatg aagctcggcc ttgacctgcg tggcggcgtt
     4201 cacttcctga tggaagtgga tatggacacc gcgcttggca aactccagga acaaaatatc
     4261 gatagcctgc gcagcgatct gcgtgaaaag ggcatcccgt ataccaccgt tcgtaaagaa
     4321 aacaactacg gcctgagcat tactttccgc gatgctaaag ctcgtgatga agccattgcg
     4381 tatctgagca agcgccatcc ggacctggtg attagtagcc agggcagcaa ccagctgcgt
     4441 gcggtaatga gcgatgctcg tctgagtgaa gcgcgtgaat atgcggtgca gcagaacatc
     4501 aatatcctgc gtaaccgtgt aaaccaactt ggcgtagcgg agccggtggt tcagcgtcag
     4561 ggtgctgacc gtatcgttgt tgaactgcca ggtattcagg acactgcgcg tgcgaaagag
     4621 attctgggtg cgacggcaac gctggaattc cgtctggtaa acaccaacgt tgaccaggcc
     4681 gctgcggcat ccggtcgcgt accgggcgac tctgaagtga aacagacccg cgaaggtcag
     4741 ccagttgtgc tgtacaaacg cgtaattctg accggtgacc atatcaccga ctccacttcc
     4801 agccaggacg aatacaacca accgcaggtt aacatctcgc tcgatagcgc tggtggtaac
     4861 atcatgtcta acttcactaa ggacaacatc ggcaaaccga tggcaaccct gtttgtggag
     4921 tacaaagaca gcggtaagaa agatgcaaat ggtcgtgcgg ttctggtgaa acaggaagag
     4981 gtgattaaca tcgccaacat ccagtctcgt ctgggtaaca gcttccgtat caccggcatc
     5041 aacaacccga acgaagcccg tcagctgtca ctgctgctgc gtgccggtgc gttgatcgcg
     5101 ccgattcaga ttgttgaaga acgtaccatt ggcccaaccc tgggtatgca gaacattgaa
     5161 caggggctgg aggcttgcct tgccggtctg ctggtttcta ttctgttcat gatcatcttc
     5221 tataagaagt ttggtctgat tgcgaccagt gctctgattg ccaacttgat cttaatcgtc
     5281 ggcattatgt cgctgttgcc aggcgcaacg ctgagtatgc caggtatcgc gggtatcgtc
     5341 ttaacccttg cggtggcggt cgatgcgaac gtactgatca acgaacgtat taaagaagag
     5401 ttgagcaacg gacgtactgt tcaacaggca attgatgaag gttatcgtgg cgcatttagt
     5461 tctatcttcg atgcgaacat caccacgctg attaaagtca tcatcctgta cgcagtgggt
     5521 accggggcaa ttaaagggtt cgcgattact accggtatcg gtgtggcgac gtcgatgttt
     5581 accgcgattg tcggtacgcg tgccatcgta aacctgctat atggcggcaa gcgcgtcaag
     5641 aagctgtcaa tctgaggagt gcgatgtggc acaggaatat actgttgaac aactaaacca
     5701 cggccgtaaa gtctatgact ttatgcgctg ggactactgg gctttcggca tctctggtct
     5761 gctgttaatc gccgctatcg ttattatggg cgtgcgcggc tttaactggg ggctggattt
     5821 caccggtggt acggttatcg aaattacgct cgaaaaaccg gctgaaattg acgtaatgcg
     5881 tgatgcattg caaaaagccg gttttgaaga gccaatgctg caaaactttg gtagcagcca
     5941 cgacatcatg gtccgtatgc cgcctgctga aggcgaaacc ggcggtcagg tgctgggcag
     6001 ccaggttctg aaggtgatta acgaatccac caatcagaat gcagcagtga agcgtattga
     6061 gttcgtcggt ccgagcgtgg gggaagacct tacgcaaacc ggtgcgatgg cgttgatggc
     6121 ggcgctgctg tctatccttg tgtacgtagg ttttcgcttt gagtggcgac tggcggcagg
     6181 ggttgttatt gcgctggcgc acgacgttat cattacgctg ggtattttgt cgttattcca
     6241 tatcgagatt gacctgacca ttgtggcatc gttgatgtcg gttatcggtt actcgcttaa
     6301 cgacagtatc gtggtatcgg accgtattcg tgaaaacttc cgcaagatcc gtcgcggtac
     6361 gccttacgaa atctttaacg tgtccttgac ccagacgctg caccgtacct tgatcacatc
     6421 cggtactacc ttgatggtta tcctgatgct gtacctcttc ggtggtccgg tactggaagg
     6481 cttctcgctg accatgctta tcggtgtttc catcggtact gcatcttcca tctatgtggc
     6541 atctgcgttg gctctgaaac tgggtatgaa gcgcgaacac atgttgcagc agaaagtgga
     6601 aaaagaaggg gcggatcagc cgtcaattct gccgtaatca agttcccgtt gatgttgaaa
     6661 atcccggtca gaagatcggg attttttttg atgtatggag attgcgaaaa tccccgcatc
     6721 ttgggaaact gcgcgtaacc ctacatttca tccaggtaac tcttcatggc tatcatccca
     6781 aaaaactatg cgcggttaga aagcggctat cgcgaaaaag cattaaaaat atatccgtgg
     6841 gtctgcggtc gctgttcccg cgagtttgtt tattccaacc tgcgtgaact taccgttcac
     6901 cacattgatc acgaccatac caataacccg gaagatggca gtaactggga attgctttgt
     6961 ctctattgcc acgatcatga gcattcgaaa tataccgaag cggatcagta tggtacgacc
     7021 gttatcgcag gggaagatgc gcagaaagat gtcggtgaag cgaagtacaa cccattcgct
     7081 gacctgaaag cgatgatgaa caagaagaag tgattaaaac gtaaaattgc ctgatgcgct
     7141 acgcttatca ggcctacgtt atttcagcaa tatattgaat ttacgtgctt ttgtgggccg
     7201 gacaaagcgt ttacgccgca tccggcatga acaaagctca cgttgttaac aatcagaaat
     7261 gccgggaata aatcccggca ttttcataat cagaagttgt aacctactac caggtaacca
     7321 ccccagccgg tagagcgaac gttgaagttg ccgttgccga agttcagttc tgcatcgtcg
     7381 ttccactgac caccgtcgtg ccagtaacga gctacgacag agtagtgcca gtgatcgtag
     7441 ttcagagcca gaatatggct ggaagcgata gagttattag tacgggtctt aataccgttg
     7501 attgcgttac cgctgtcatc ccctaaatcg gaaccccagt cgaagttggt gaagccgatg
     7561 tagctcagct gaccgcccca cagatcggta atcggcacaa agtatttaat tttgaaacgg
     7621 taaccgtccc actcgttttc gttcgctgcg ccatagttct gccactggta tttcgcatag
     7681 acgttcatgg acaggctcat cggcagatca gtatcgatgt cggtacccag acccatgtac
     7741 caggtgctct ggcgaccatt tttattacga cccatgtcgt aaatgtagtt gttcgcgaag
     7801 taccactctt tgaacggacc gaagttaagg tcagtattgg tcagcttgtc gatggagaaa
     7861 cgtggttcga tttccataaa cagcggagaa ccgtggttcc agataccttt agcatcggag
     7921 ttaccgccga agaataccgg cgcatccgca taaccataga agtcgaacca gtctttttta
     7981 gcgaatgctt cgtactcaag gtaggtatcg ttgcggatct gcggtccgaa acgggtgtga
     8041 tagcttccga caacgttaac gctctggtgc caccagtcgg aaagatactg cggtttgtcg
     8101 ttttcagctg cgttgacagt aaaagacgaa gagagcgcca gtaccgcacc ggctgccagt
     8161 aatgtttttt tcatatgtat gccactgttt gaaaatccct tgcgggagtg aaaaaggcgc
     8221 aaattgcgtt tctaaatatt tcgtatacag aggagcctat tataaaaatc attgctcaca
     8281 aaaatatgtt tcgtttcaca gttctatcat ttacgtaatc gattgcgttc acgtttacac
     8341 acattcgggc ggggattgta ctgactttcg cccttgttgc aaacttttac tatcaatcca
     8401 ggtgatgtaa gcagagtaac aaaatgacgg ggaaagatgt tgcgggacgc acaaaatcgt
     8461 cccgaaatac gtttactgca taacaggttg aatgtcgtgg atacgtacaa agcctaactg
     8521 atccacagaa atccccttta attgtaacgg aatatcaaca tcgctggggg cgaggacgct
     8581 ggcaggggca ttaatcaatt gattctgcac attgatttcc tgaaagttgt ctattgttcc
     8641 ctgaatttgc ccatattcaa cagttccgct gaatgcgggc aagggatcat tcgactcacc
     8701 ctgaatacgt agtgtaaggg tggtgccatc tgtatcaggc gtaatattca ccagcgacat
     8761 acgtaaagta ccgatctggc tttccagtct tgccggtgtt ttcgctctag gcagaaggta
     8821 acccccactg ctggattttg cattcagcct gttttgctga gtgattttga ctgtttcttg
     8881 attgagttgc gtcatctcct ggttcagcgt gctgacgctg tgtttcatct gtcgcacttc
     8941 actttgctgc acacaggcgc taaggctgaa caggcttccc agcaggagca gtcgaaaaac
     9001 gttagtattc ataggtcgtc cctttctcgt cgcaattagc ctaatggtag agggtaaaag
     9061 cagcgaaagc atcgatcctt tatctcaaaa gcgttgcgcc tttgttgtat cgtcagttca
     9121 gggtaaaata gatttccgtt aaccacttgg tcaggacgcc gtatgcattg cccattctgt
     9181 ttcgccgtgg acactaaggt aattgactct cgtctcgtgg gcgagggttc atccgtacgc
     9241 cgccgtcggc agtgtctggt gtgtaatgaa cgtttcacca cctttgaagt ggcggagctg
     9301 gttatgccgc gtgttgtaaa aagcaacgac gtgcgtgaac cgtttaatga agagaaattg
     9361 cgtagcggaa tgctgcgggc gctggaaaaa cgtccggtga gttccgatga cgttgaaatg
     9421 gcaatcaatc atattaaatc gcagctgcgc gccaccggtg agcgcgaagt accgagcaag
     9481 atgattggca atctggtgat ggagcaattg aaaaagctcg ataaagtcgc ctatatccgt
     9541 tttgcctctg tctaccgcag tttcgaagat atcaaagaat ttggcgaaga gatcgcgcgc
     9601 ctggaggact aagccgtgca ggacgagtat tacatggcgc gggcgctaaa gctggcgcaa
     9661 cgaggacgtt ttaccaccca ccccaaccca aatgtcgggt gcgtcattgt caaagatggc
     9721 gaaattgtcg gtgaaggtta ccatcaacga gcgggtgaac cacatgccga agtacacgcg
     9781 ttgcgtatgg cgggtgaaaa agccaaaggt gcaacggcgt atgtgacact cgaaccttgc
     9841 agccatcacg gccgtacgcc gccttgctgt gatgctttaa ttgccgcagg tgtggcgcgc
     9901 gtggttgcct cgatgcaaga tcctaacccg caggtcgctg ggcgtggact ttaccgtctg
     9961 caacaggctg gcattgacgt cagccacggc ctgatgatga gtgaagccga gcaattgaat
    10021 aaaggctttc tcaagcggat gcgcaccggc tttccttata ttcagttaaa acttggcgca
    10081 tcgcttgatg gtcgcacggc gatggcgagc ggcgaaagcc agtggatcac ttcgccccag
    10141 gcgcggcgtg atgtacaacg actgcgcgcg caaagtcatg ccattttaac cagcagcgcc
    10201 acggtgctgg cggatgatcc tgccttaacg gtgcgttggt ccgaactgga tgaacaaact
    10261 caggcgctct acccgcaaca aaatctccgc cagccggtac gtattgtgat tgatagccaa
    10321 aatcgcgtga cgccggaaca tcacattgtg cagcagcccg gcgaaacctg gttcgcgcgt
    10381 acgcaggaag attctcgtga gtggccggaa acggtgcgta ccttgctgat tccagagcat
    10441 aaaggtcatc tggatctggt tgtactgatg atgcaactgg gtaaacagca gattaacagc
    10501 atctgggggg aagcggggcc aacgctcgct ggcgcattgc tgcaggcggg tttagtcgat
    10561 gagctgattg tctatatcgc acctaaacta ttaggcagcg acgcccgtgg attatgcacc
    10621 ctgccagggc ttgagaaatt agccgacgcc ccccaattta aattcaaaga gatacgtcat
    10681 gtaggcccgg atgtttgcct gcatttagtg ggtgcatgat ctcccggctc gaaagggaag
    10741 cagcgcacga aatattatgc taaaatccgc ccccctgcgg ggccatactc gaacccgaag
    10801 gaagaaaatg aacattattg aagctaacgt tgctaccccg gacgctcgcg tcgccatcac
    10861 catcgcgcgt ttcaacaact ttatcaatga cagcctgctg gaaggtgcaa ttgacgcact
    10921 gaaacgtatc ggtcaggtaa aagatgaaaa cattaccgtt gtttgggtgc ctggtgccta
    10981 tgagctgccg ctggcggcgg gtgcactggc taaaaccggt aaatacgacg cggtgattgc
    11041 gctgggtacg gttattcgtg gtggcactgc ccattttgaa tatgtcgctg gtggtgcaag
    11101 caacggcctg gcgcatgttg cccaggacag cgaaattccg gttgcttttg gggttctgac
    11161 cactgaaagc attgaacaag cgatcgaacg tgctggcacc aaagctggca acaaaggtgc
    11221 agaagctgca ctgaccgcgc ttgaaatgat taatgtattg aaagccatca aggcctgaaa
    11281 ttagtaaggg gaaatccgtg aaacctgctg ctcgtcgccg cgctcgtgag tgtgccgtcc
    11341 aggcgctcta ctcctggcag ttgtcccaga acgacatcgc tgatgttgaa taccagttcc
    11401 tggctgaaca ggatgtaaaa gacgttgacg tcctgtactt ccgtgagctg ctggccgggg
    11461 tggcgactaa taccgcatac ctcgacggac tgatgaagcc atacctgtcc cgtctgctgg
    11521 aagaactggg acaggtagaa aaagcagtac tgcgcattgc gctgtacgaa ctgtctaaac
    11581 gtagcgatgt gccatacaaa gtggccatta acgaagcgat cgaactggcg aaatcgttcg
    11641 gcgcagaaga cagccataag ttcgtcaacg gtgtactcga taaagcagca cctgtgattc
    11701 gccctaacaa aaagtaatat ccaggccggt agattcacgg aagaccgttc catgatcgcc
    11761 ggccttttct tttttacctg ctgaggcata acgtatggca tgtggcgagt tctccctgat
    11821 tgcccgttat tttgaccgtg taagaagttc tcgtcttgat gtcgaactgg gcatcggcga
    11881 cgattgcgca cttctcaata tccccgagaa acagaccctg gcgatcagca ctgatacgct
    11941 ggtggcgggc aaccacttcc tccctgatat cgatcctgct gatctggcgt ataaagcact
    12001 ggcggtgaac ctaagcgatc tggcagcgat gggggccgat ccggcctggc tgacgctggc
    12061 attaaccttg ccggacgtag acgaagcgtg gcttgagtcc ttcagcgaca gtttgtttga
    12121 tcttctcaat tattacgata tgcaactcat tggcggcgat accacgcgtg ggccattatc
    12181 aatgacgttg ggtatccacg gctttgttcc gatgggacga gccttaacgc gctctggggc
    12241 gaaaccgggt gactggatct atgtgaccgg tacaccgggc gatagcgccg ccgggctggc
    12301 gattttgcaa aaccgtttgc aggttgccga tgctaaagat gcggactact tgatcaaacg
    12361 tcatctccgt ccatcgccgc gtattttaca ggggcaggca ctgcgcgatc tggcaaattc
    12421 agctatcgat ctctctgacg gtctgatttc cgatctcggg catatcgtga aagccagcga
    12481 ctgcggcgta cgtattgacc tggcattgct gccgttttct gatgcgcttt ctcgccatgt
    12541 tgaaccggaa caggcgctgc gctgggcgct ctctggcggt gaagattacg agttgtgttt
    12601 cacggtgccg gaactgaacc gtggagcgct ggatgtggct ctcggacacc tgggcgtacc
    12661 gtttacctgt atcgggcaaa tgaccgccga tatcgaaggg ctttgtttta ttcgtgacgg
    12721 tgaacctgtt acgttagact ggaaaggata tgaccatttt gccacgccat aaagatgtcg
    12781 cgaaaagtcg cctgaagatg agtaatccgt ggcatctact tgctgtcgga ttcggaagtg
    12841 gattaagccc gatcgttcct gggacgatgg gctcgctggc agcgattccg ttctggtatc
    12901 tgatgacctt tttgccctgg cagctctact cgctggtggt gatgctgggg atctgtatcg
    12961 gcgtctatct ttgtcatcag accgcaaaag atatgggcgt gcacgatcac ggcagcattg
    13021 tctgggacga atttattggt atgtggatca cgctcatggc gctgccgacc aatgactggc
    13081 agtgggttgc cgccgggttt gtgattttcc gtattctgga tatgtggaaa ccgtggccga
    13141 tccgctggtt tgatcgcaat gtgcatggcg gcatggggat catgatagat gatattgtcg
    13201 ccggggtgat ctccgctggc atcctgtact ttattggtca tcactggccg ctgggcattc
    13261 tgtcgtagtt gtgcaccgat gcctgatgtg acgcttgttg cgtctcatca ggcctggact
    13321 cttatttaaa tcctactacc ggatgcggtt tatacggcgt ttccagttcg gcaatctgtt
    13381 ccggcttcaa agtgatatcc accgcgttca atagctcatc aagctgttct tcccgcgatg
    13441 taccgataat cggtgcggca atgcccggtt tactcaacaa ccaagccagc gcaacttgtg
    13501 ctcgtgttgc accgagttct tcactgacgc ccgttaaccg ctctgcgatc tgcgcgtcat
    13561 tttcatcgct ttctttatag agatttttcc ccacctcatc agacaccagt cgtgcggtag
    13621 tttctcccca cggacgcgtc agtcgccccc gcgccagcgg gctccacgga atcaccgcca
    13681 cgccctcctg ataacacagt ggcagcatct cgcgctcttc ttcgcgataa atcagattgt
    13741 agtgatcctg catactgaca aactgcgccc agccgtgctg tttttggagt tccagtgcct
    13801 gagcaaactg cgaagcgtgc attgatgacg cgccgatata acgcgctttc ccggctttta
    13861 ccacgtcgtt cagggcttcc agcgtctctt cgatcggcgt gttgtaatcc cagcgatgaa
    13921 tttgcaggat atcgacataa tccatgccga gacgtcgcag gctgtcgtcg atagagcgca
    13981 aaatttgtgc acgggataat ccttccggta aatcaccaac gcgatggaac actttggtgg
    14041 caacgaccac gtcttcacga cgggcgaaat cccgcagtgc gcgaccaacg atctcttcgc
    14101 tgctaccatc ggaatagcta ttggcggtat caaagaaatt tatgccgcct tccagtgcgc
    14161 gtttaattat gggacggctg ctctcttccg gcagtgtcca tgcgtgatta ccacgatctg
    14221 gctcgccaaa ggtcatacag ccgaggcaaa gtcgggaaac gcgaaggtcg gtttttccta
    14281 aggggttgta ttgcatgctg ccactcctgc catgaataat ttcttaagca tagcaggagt
    14341 ggagtaggga ttatgccagc caggccttga ttttggcttc cataccggcg gcatcgaggc
    14401 cgagttcggc gcgcatttct tcctgcgttc cttgcggaat aaagaagtca ggcaggccaa
    14461 tgttcagcac ggggactggt ttacgatggg ccattagcac ttcgttcact ccgctgcctg
    14521 caccgcccat aatggcgttt tcttctacgg taaccagtgc ttcatgactg gcggccattt
    14581 ccagaattaa cgtttcatca agcggtttca caaaacgcat atcgaccagc gtggcgttca
    14641 gcgattcggc gactttcgcc gcttctggca tcagcgtacc aaagttaagg atcgccagtt
    14701 tctcgccacg acgcttcaca atgcctttgc caattggtag tttttccagc ggcgtcagtt
    14761 ccacgccgac cgcgttgcca cgcgggtagc gcaccgctga cgggccatcg ttatagtgat
    14821 agccggtata gagcatctgg cgacattcgt tttcatcgct cggggtcata atgaccattt
    14881 ccggtatgca gcgcaggtaa gagagatcaa aagcaccctg atgggtttga ccgtcagcac
    14941 caacaatgcc cgcgcggtcg atggcgaaca ggaccggaag cttttgaatt gccacgtcat
    15001 gcagcacctg atcataggcg cgttgcagga aagtggagta gatcgcgaca atgggtttgt
    15061 acccaccaat cgccagaccg gcggcaaagg tcactgcgtg ttgctcggca attgccacgt
    15121 cgaagtagcg atccgggaat ttacgagaaa actcgaccat gccggaacct tcacgcatcg
    15181 ccggagtaat cgccatcagc ttgttatctt tcgccgccgt ttcgcacaac cagtcgccaa
    15241 agatttttga atagctcggt aaaccgccgc tacttttcgg caaacaaccg ctggagggat
    15301 caaatttagg cacggcgtgg aaagtgattg ggtctttttc tgccggttca taaccacgac
    15361 cttttttggt catgatatgc aggaactgcg ggcctttcag gtcacgcatg ttcttcagcg
    15421 tggtgataag ccccagcaca tcgtgaccgt caaccgggcc gatgtagtta aagcccagct
    15481 cttcaaacaa cgtgccaggg actaccatgc ctttaatatg ttcttcggta cgtttgagca
    15541 gctctttaat gggtggcacg ccagagaaaa cttttttccc gccttcgcgc agtgaagagt
    15601 aaagcttacc ggaaagcagc tgcgccagat ggttgttgag cgcgccgaca ttttcggaaa
    15661 tcgacatttc gttgtcgttg aggaccacca gcatatcagg acggatatcg cccgcgtgat
    15721 tcatcgcttc aaacgccatg cctgcggtaa tcgcgccatc gccgattaca cagacggtac
    15781 ggcgattttt gccttctttc tcggcagcaa ccgcaatacc aattccggca ctgatggagg
    15841 ttgatgaatg cccgacgctt aatacgtcat attcgctttc gccgcgccac gggaacgggt
    15901 gcaggccgcc tttctgacgg atggtgccga ttttgtcgcg gcgtccggtc aaaattttat
    15961 gcggataagc ctgatgcccc acatcccaaa tcaattggtc aaacggggtg ttgtagacat
    16021 agtgcagcgc cacggtcagt tcgaccgtgc ccagcccgga ggcgaagtgc ccgctggaac
    16081 ggctcacgct gtcgagtaaa tagcggcgca gttcgtcgca gagtttcggt aaactctctt
    16141 tcggcaacag tcgtaactcc tgggtggagt cgaccagtgc cagggtcggg tatttggcaa
    16201 tatcaaaact catcaggggc ctattaatac ttattgttta tttattacgc tggatgatgt
    16261 agtccgctag cgcttccagt gccgaggtat cgggtgactg ttcagccagt tgtttcagcg
    16321 actgacgggc atcgtcgatc agatcccggg ctttcttccg ggcttgctca agacccagaa
    16381 gtgcagggta ggtactttta ccaagttgct ggtcggcacc ctggcgtttt cccaacgttg
    16441 cagtatctcc taccacatcc aggatgtcat cctgaacctg gaaggcaagg ccgatgctct
    16501 ctgcgtactt gtcgagtact ggcagagcac gacgcccttt atctccggcg cttaatgcac
    16561 caaggcgaac ggcggcgcga atcaatgcgc cggttttatg acgatgaata cgctcaagcg
    16621 cgtccagagg tacgtgtttg ccttccgcgt ctaaatctaa tgcctgacca ccgcacattc
    16681 cggcaatacc gctggcgctc gccagttcag aaatcatcga aattctgtcg cgatccgaca
    16741 cttccggcat atcagcatcg cttaaaatcg agaacgccag cgtttgtaaa gcgtcgccag
    16801 cgagaatcgc gtttgcttcg ccaaacttca catggcaggt cggcaaaccg cgacgcagat
    16861 cgtcatcatc catcgccggt aaatcatcat gaattaatga gtaagcgtgg atacactcta
    16921 cggcggcagc gggtgcgtcc agcgtgtttg tgctaacgcc aaacatatga ccggtggcat
    16981 aaaccaggaa gggtcgcaga cgcttaccac ctaataatgc accatactgc atggtttcga
    17041 ccacgggagt gttctgaaag ggcagtgggg cgataaaacg gctcagcgcc tggttggcct
    17101 gcttaacgca ggcttcgagt tgctgcggaa agtccattac tcattgtccg gtgtaaaagg
    17161 ggttagagag gcgtcttcat tgtcagacag cagaatttgt acgcgctgtt cggcttgttg
    17221 taatttggcc tgcccctgac gtgccagctg cacgccgcgt tcgaactcgt tcagcgcctc
    17281 ttccagcggc aggtcgccac tttccagacg ggttacaatc tgttccagct cgctcagcgc
    17341 cttttcaaag ctggcgggag cctcattttt cttcggcata atgaatgtct gactctcaat
    17401 attttttgcc ccgtcatggt aacggactca gggcaaatag caaataacgc gcaatggtaa
    17461 ggtgatgtgc gcagcaaagc gatgttagtg gtatacttcc gcgcctggat gcagccgcag
    17521 gtgtgggctg ctgtattttt ccctatacaa gtcgcttaag gcttgccaac gaaccattgc
    17581 cgccatgaag tttatcatta aattgttccc ggaaatcacc atcaaaagcc aatctgtgcg
    17641 cttgcgcttt ataaaaatcc ttaccgggaa cattcgtaac gttttaaagc actatgatga
    17701 gacgctcgct gtcgtccgcc actgggataa catcgaagtt cgtgcaaaag atgaaaacca
    17761 gcgtctggct attcgcgacg ccctgacccg tattccgggt atccaccata ttctcgaagt
    17821 cgaagacgtg ccgtttaccg acatgcacga tatttttgag aaagcgttgg ttcagtatcg
    17881 cgatcagctg gaaggcaaaa ccttctgcgt acgcgtgaag cgccgtggca aacatgattt
    17941 tagctcgatt gatgtggaac gttacgtcgg cggcggttta aatcagcata ttgagtccgc
    18001 gcgcgtgaag ctgaccaatc cggaagtgac tgtccatctg gaagtggaag acgatcgtct
    18061 tctgctgatt aaaggccgct acgaaggtat tggcggtttc ccgatcggca cccaggaaga
    18121 tgtgctgtcg ctcatttccg gtggtttcga ctccggtgtt tccagttata tgttgatgcg
    18181 tcgcggctgc cgcgtgcatt actgtttctt taaccttggc ggcgcggcgc atgaaattgg
    18241 cgtgcgtcag gtggcgcatt atctgtggaa ccgttttggc agctcccacc gcgtgcgttt
    18301 tgtcgctatt aatttcgaac cggtcgtcgg ggaaattctc gagaaaatcg acgacggtca
    18361 gatgggcgtt atcctcaaac gtatgatggt gcgtgccgcg tctaaagtgg ctgaacgtta
    18421 cggcgtacag gcgctggtca ccggcgaagc gctcggccag gtgtccagtc agacgctgac
    18481 caacctgcgc ctgattgata acgtctccga tacgctgatc ctgcgtccgc tgatttctta
    18541 cgacaaagag cacatcatca acctggctcg ccagattggc accgaagact ttgctcgcac
    18601 gatgccggaa tattgcggtg ttatctccaa aagcccgacg gtgaaagcgg ttaagtcgaa
    18661 gattgaagcg gaagaagaga aattcgactt cagcattctc gataaagtgg ttgaagaagc
    18721 gaataacgtt gatatccgcg aaatcgccct gcagaccgag caggaagtgg tggaagtgga
    18781 aaccgtcaat ggcttcggcc cgaacgacgt gatcctcgat atccgttcta tcgatgaaca
    18841 ggaagataag ccactgaaag tcgaagggat tgatgtagtt tctctgccgt tctataaact
    18901 gagcaccaag tttggcgatc tcgaccagaa cagaacctgg ctactgtggt gtgagcgcgg
    18961 ggtgatgagc cgtctgcagg cgctctatct gcgcgagcag ggctttaaca atgtgaaggt
    19021 atatcgcccg taatttgtgg ttgtaacgtc gcatctggtc agatgcgacg cttaccgcat
    19081 ccgacactac tcgtaataat tataaatccc tgccgccatc accagttgtg atgccacttc
    19141 atgggctttc tcgcgcccaa cctgcaggtc gataattttc agaccaaagt cgatagctgt
    19201 ccccggcccc tggctggtca gcaattttac ccgtgcatcc cagacgacgc gcttgtcctg
    19261 ccattgttca gcgggaattt tgtctttcag cgtcgggaag ccggtcatat taccaatcgg
    19321 gaagatatcg tgcggcacca gcacggtggc tggcgcggcg caaatagccg cgacgatacg
    19381 cccggaacgg tggaactgtt taacggtttc aaccagcaga gtgctatcgc gaaaacactc
    19441 tgcgccttta atgccaccag gcagtacgat cacgtcatat tcgccatcag ccacctcgac
    19501 cagcggcgca tccgccagca gcttcacgcc gcgcgagcag gtaattgcca ggttgccatc
    19561 gctggctacg ctggcagtgg tgactttgat accgccgcga accaagaggt cgatcgtggt
    19621 gacggcttca gtctcttcac taccaggggc gaggcaaacc agtgccgatg cgctcatatt
    19681 cactctcctt tctttttacc atttcaaaca ggcgggtgtt ttccggtacg gcaatcccat
    19741 gcgcgcgggc gcggcgtaag agaaaaccat tgatatagtc gatttcggtg tggcgcagcg
    19801 cgcggatatc ctgcaacatc gacgagatat tttccgctgt ggcatcaatc acctgcatca
    19861 cgtaatcacg caaatcttcc gctgaagtat gatgcccttc gcgttcgatc actgccgcga
    19921 cttcttcgca tatttgcata atttcttgcg gatgatgacg taattcaccg ttcgggcaat
    19981 tccagatggc ggtcagtgga ttaatcacgc agttgactgc cagcttgcgc cacagctcgg
    20041 cgcgaatatt gttatgccag gcaacgtcag gcaacacggt ttgtaaaata tccgccagat
    20101 aactgtaatc gccatcctgt tgccgtgccg gaccaatatg cgtgataccg tttgccacat
    20161 gaataatgac attgccgtcg cggcgggcgg catgggtggt ggtgcccatc agtaatggct
    20221 gctgaatatt ttgcaactct tcgatggtgc ccatgccgtt gtgaattaac agtattggcg
    20281 tggttacagg cagtgtggac gcgaggcttt tgacggcatc ggaaacctgc catgctttca
    20341 gcgtcaccag gagcagatcg ctggtggcga gaaaatcggg atcgttggcg gtcagcgatt
    20401 cgttaaatat cgaaccatct gtctcaacca gattcgcact acaataaggt tgcggtacgc
    20461 gcagccagcc ctgaacttca tgaccctgtt tgcaaagtgc tgtaagccat aattgcccta
    20521 aggcaccgca tcccaatacg gtaattttca ttgttcctcc tcacccgcaa ccactccggg
    20581 tgttcaataa ggctatccct taattgtgca tgctgttgcg actatgcaca attaagggat
    20641 acgtcctggt gcaggactgt cggttattta actttgcggg tattatgctt cgcatcaaaa
    20701 atgaagggag aggaaaagat gccatctttc gatattgtct ctgaagttga tcttcaggaa
    20761 gcacgtaacg cggtcgataa cgcgagccgc gaagtggagt cccgttttga cttccgtaac
    20821 gttgaagcct catttgagct gaacgacgcc agcaaaacca tcaaagtgtt gagcgagtcc
    20881 gatttccagg tcaatcagtt gctggatatt ctgcgtgcca agctgctgaa gcgcggcatt
    20941 gaaggcagtt cactggatgt accggaaaat atcgttcata gcggtaaaac ctggtttgtg
    21001 gaagcgaaac tgaagcaggg cattgagagc gcgacccaga aaaaaatcgt caagatgatc
    21061 aaagacagca aactgaaagt gcaggcgcaa attcagggcg atgagatccg cgtgactggc
    21121 aaatctcgtg atgatttgca ggctgtcatg gcgatggtgc gcggtggcga tctgggtcag
    21181 ccgttccagt tcaaaaactt ccgcgattaa tcgcgacaac ctgcgctttg ttcatgtcag
    21241 atgcggcatg aacgcctggt tcggcctaca aaggcttgta aatcaggagc ttagtaggcc
    21301 tgatgagcgt agcgcagcag gcaatttcgc ctcgctttta tgcctggcga attgcctgtt
    21361 caacatcaaa gcgattcgtc actttgctgt cgattttcac ataagctgaa tgttcttctt
    21421 ctgcaatcaa cacttctttg atgccttcag tttctagcaa acgcactttt aacgcctcgt
    21481 ttgcggcaat gttcgccggg atttcaatgc gcaaactgct gacatacgcc ggttctttca
    21541 tggtactggc gactgccagc cacactgcgg ccagcattgc gccagcgaga aatacgccct
    21601 gaccgtcaaa catgccgtca atccagccgc ccagtgaacc gccaatcgcc acgccaagaa
    21661 actggctggt ggagtaaaca cccatcgctg tacctttgta acctgctggc gactctttac
    21721 tgataagtga aggcagaagg gcttccatca aattaaacgc tacaaagaaa agctgcacgc
    21781 cgaccaccag ttgccagaac tgcgtttgcg cgttccacaa cacaatttcc gcaaccacga
    21841 tcaatccgac gcagaagaca aagacttgct tcattttgcg cttaacttca gcgtagataa
    21901 tgaaaggcac gaccgagcca aaggcgatta gcattgtcgc cagatagacc ttccagtgtt
    21961 cagccgccgg gaaccctgca tcagccagtt gtccgggcag ggcaacaaac gtcgacatca
    22021 gcaaaatatg cagacacata atgccaaagt tgagtttcag cagccgcggt tccgccagca
    22081 ctttactgaa actgcctttc accattccgg actcacgatt aagtacgtga gtgctactgt
    22141 tgggcacaac ccaaatggtc aacgcaatgc cggtcgttgc cagaatagcg atcatccaga
    22201 acagcgcgtg cagcccaagt ttgtgagtga tgatcgggcc aagcaccatc gcaatggcaa
    22261 aggtaatgcc aaagctcacg ccgataaacg ccattgcttt ggtgcggttt tgttcgcgcg
    22321 tgagatcgga aagcagcgcc ataacggcag cggcaatcgc accggagcct tgtagcgccc
    22381 ggcctagaat aattccccag atggagtcag agagcgcagc gataacgcta ccggcggcaa
    22441 acaccgccag cccaccgaca attaatggtt tgcgaccaat acggtcggaa agcaggccaa
    22501 acggaatctg aaaaacggcc tgagtcagac cataaatacc aatggcaata ccgattaatg
    22561 cttcgctggc accttgcaga gccatgccat acgtggtcag aaccggcaga accataaaca
    22621 tgcccagcat gcgcaacgag aatacggtcc ctaaacccca ggtcgcgcgc ctctcacctg
    22681 gcgtcatttt ataatcgttc attaccacct ctgttttaaa ttcgcgacta gtgtaaagcg
    22741 gcaaacgaat ggggtaaaca tgcggatttt ttagtaaata tttcgagagg gatatgtttc
    22801 taatgctaag aaaaaaggtg ccgtagcacc tttttaatag agaggttttg ttaccacaca
    22861 gcagccagca gcgtatgcga gtccggtacc ataaaatcaa cggacatcat caccgagagg
    22921 gcagtgatgg cgatgataga gaagccgaac agcttgcgcg cccagattct gtcatcagca
    22981 actttataac cgcgcagagc catacctaac caccagacgc taaccgccgc ggcgaccacc
    23041 agatatttat atccagcgta accgccaaga gagagcatca gcgtggcaac ggcaaaggcg
    23101 atgatataca gcgtgatgtg attcttcgcc accgaaatgc cttttaccac tggcaatacc
    23161 ggaatgtttg ccgcctggta atccttaaag cggaaaatgg cgatggcata ggagtgaggc
    23221 atctgccaca ggctgaagat agccagcagg atcgctgcgc cgctatcgaa ctcgccggtt
    23281 actgcacagt agccaatcac cggcggcgca gcgccggaga gcgaaccaat caatgtgccg
    23341 tagactgagt ggcgtttcat gtacaggcta tagacgccga cataaaccac aaagcccatc
    23401 acccccagcc agcaggccag cggattcgcg ccaaaccaca gcagcataaa gccagcaata
    23461 cccagcaagg tggcgtacac cagcgagaca gcaggagaga tcaggccttt aaccagcacc
    23521 cgattcttcg tcctttccat ctttctgtcg atatccctgt cgatgtagtt gttaaacaca
    23581 caacccgacg ccacaaccag tgacacccca accagcgtgt agataaacag gggataatca
    23641 atgctgccct ttgaggccag caggaatccc ccaatcaccg agatcaggtt gccaaagatg
    23701 atgcctggtt tcgttacttg caggtattgc ttaaacatca taaccgccgc tcttagtgca
    23761 tcatcatgtt gtagttgagg ttccacataa tccagatgga gcctacaacc aggatagtga
    23821 tgattagcac ggtgaagaca aacgccgtca tgttccagcc ttcatctgat ttggtattca
    23881 tgtgcaggaa gcacaccaga tgcaccagaa cctgtaccac tgccattgcc aggattgttc
    23941 ccagaattac ggccggagag gcagcccctg tcatcaccat ccagaacgga atcaccgtca
    24001 gaatgatcga caggataaag cctgtcatgt aggtttttac gctgccatgg gacgcgccgc
    24061 tgtgatcggt agaatgactc attacatcgc ccccatcaga taaacaacag tgaacacaca
    24121 gatccaaacc acatccagga agtgccagaa caggctcagg cacatgatgc gggtacggtt
    24181 agtgctggtc aggccgcgac gagcgatttg taccatcagc accgccatcc agataagacc
    24241 ggaagtgacg tgcagaccgt gcgtgccgac cagcgcaaag aacgctgaca ggaagccgct
    24301 gcgatccgga cccatgccgt taacaatcag gtgatggaat tcgtagattt ccatcccgat
    24361 aaatccggca ccaaacaaac aggtcaacgc cagccaggag ataacctggc ttttgttgtt
    24421 tttgtacatg gcgatagccg ccatgccgta ggtgatggag ctgaacaaca gcaagaaagt
    24481 ttcaaccaga acgaacggca gttcgaaaat gtccttacct gtcgggccgc ctgcggtgcc
    24541 gttcaccaga acggcatagg tagcaaacaa gattgagaac agaatgcagt cgctcatcag
    24601 gtagatccaa aatccgaaga ttttggttcc gcctgcatcg tggtgcccgt gttcgtgcgc
    24661 gtgggcagtc gcgtgcgtca aagtatcagt tgccattttt cagccctgcc ttagtaatct
    24721 catcgaaatg ctggttttcc agtttttcga tttctgccac cggcacgtag taatccacgt
    24781 cctcgtcgaa gcttttcacg atccaggtga tgatcatgcc tgcgaagcca acaatcgcca
    24841 gccaccagat atgccagatc atggcgaaac cgaagatggt ggagaaagct gcaatgacga
    24901 tacctgcacc gctgtttttc ggcatatgaa tttcttcata gtggtcaggc tttttatacg
    24961 cttcgccttt ctctttcatt tcccagaatg catcacgctc gtgaacgtgc ggcacaacgg
    25021 caaagttata gaacggaggc ggggaagagt ttgcccactc cagcgtacgg ccaccccacg
    25081 ggtcgccagt caggtcacgg ttctggtcgc ggtcgcgaat agaaacgtac atctgaataa
    25141 cgaggcagag aatacccagc gcaatcagta ctgcaccgct agctgcaatc atcagcatgg
    25201 tgtggaactg cgggtcaatc tgctggctca aacgacgggt catgcccatg aagcccagcg
    25261 catacagcgg cataaaggca acgaagaagc cgatgatcca gaaccagaac gcgcgtttac
    25321 cccaggtttc attcagtttg aaaccgaacg ctttaggcca ccagtaggtc atccctgcga
    25381 agcagccgaa gaccacgccg ccgatgataa cgttatggaa gtgcgcaatc aggaacaggc
    25441 tgttatgcag aacgaagtcc gcgcccggta ctgccagcag cacgccagtc atcccgccca
    25501 ccgagaaagt gacgataaaa ccgatggtcc acagcatcgc agaatggaac acgatacggc
    25561 cctgatacat ggtgaacagc cagttgaaga tcttcacccc ggtcgggatg gcgataatca
    25621 ttgtggtgat accaaagaag gcgtttacgt tcgcgcccgc acccatcgta aagaagtggt
    25681 gcagccaaac gatgaacgac agcacggtga tacagacggt tgcccatacc agcgaggtat
    25741 aaccaaacag acgtttacgc gagaaggttg ccgcaatttc ggagaacaca ccgaaaacag
    25801 gcaggatcag gatgtaaact tccgggtggc cccaggccca aatcaggttg atgtacatca
    25861 tcatgttgcc acccatatcg ttggtaaaga aatgggtgcc cagatagcga tccagggtca
    25921 acaacgcgac ggtaaccgtc agaattggga aggaagcaat aatcagtacg ttcgcgcaca
    25981 gtgatgccca ggtaaatact ggcatcttga acatggtcat gcccggtgcg cgcatcttca
    26041 gaatggtaac gaagaagttg ataccggtaa gcgtcgtacc tataccggat agctggagac
    26101 tccatatcca gtaatcaaca ccgactcccg gactgtactc tattcccgat agcggtggat
    26161 aggccagcca gccggtctgc gcaaattcgc ccacgccgag agaaacgtta accagaatca
    26221 caccaacaac agtaaaccag aagcttaagt tgttgaggaa cgggaacgca acgtcacgcg
    26281 cgccgatctg cagcggaacc accaggttca tcagaccgat aacgaaaggc atcgccacga
    26341 agaagatcat aatcacgccg tgcgcggtga agatctgatc gtagtggtga ggtggcagga
    26401 agcccgcttc gcccgccgag gcaagagcct gctggctacg catcataatg gcgtcagcaa
    26461 aaccacgcag caacatcaca atcgccacga tgatatacat gataccgagg cgtttatggt
    26521 cgacggaggt cagccactct ttccacaggt aggtccactt accgaagtaa gtgatcaggc
    26581 caacgagcgc cagacctccc aaaataatgc cagcgatcgt aaccatgacg ataggttcat
    26641 ggaacgggac tgcatcaagt gataattttc cgaacatctt tattcttcct caaccccttt
    26701 aatgggcgga ttccgcgtgg ctcatgtcca tgccttccat accttcgtgt gcgctgtgct
    26761 caccttctgg ctgggtcatg tccatgctct taccgtgagc cataaacttg ttaatcacat
    26821 cagcaaacaa gtctggtttc acgttggaga aatattccac ctggttgtat tcgctaggcg
    26881 cggccagttt ttcgaacgca gccatgtcag acatggtgtt cggcgactgc ttcgcttttg
    26941 cgacccactg gtcgaattcg gcgcgatccg gtgttgcaat agctttgaac ttcatgcctg
    27001 agaagcccgg gccgctgtag ctggcggaga taccgtcata agtaccgggt tcgttggcga
    27061 tcagatgcag gcgagtctgc ataccagcca tggcataaat ctggctaccc agacgcggaa
    27121 tgaagaagga gttcatcacg gagttggagg tcactttgaa gtacaccgga gtgttcgccg
    27181 ggaaagcgat ttcattcacg gtagcaatgc cctgttccgg gtagatgaag aaccatttcc
    27241 agtccatgga aaccacttcg atggtaatgg gcttctcgtc gtgtgccagc ggcttgctag
    27301 gctcaagagc gtgagtggtt ttccaggtca gtaccgcaag gaagatgatg attaagatag
    27361 gtaccgtcca gaccacagct tccactttat tggagtgtga ccagttcggg ctgtacttag
    27421 catctttatt gctcgcacga tacttccagg cgaaaccaac agccatcaag attgcgggaa
    27481 taacgacaat caacatcagg ccaaatgccg tcagtatcag tgaacgttgc tccagaccaa
    27541 tctgtccttt gggatctaac agcgcagaat tacagccact gagcaataca gtgcctgcaa
    27601 ataatgacaa ccatcccaaa cttttattgt atttcctgag tctcatttaa cgacctcaat
    27661 tccacgggat ctggtggcgt ttaaagtgtg tgggcatttt atgggaaggt tacattactg
    27721 taaacatgat taaattcctg ttacttggat ttgattgtca ggtcacatat gttgcaaaac
    27781 atatcaagtt gattttaaga agctgttgca aaagggcgtt ataacgaaag gggagtgcag
    27841 aaggagtttg gatgggcaat tggtataacc aatctggaat aaaataattg tttaacaaat
    27901 aattatcatt atggtgacaa ttgggtgggg ggaaaataaa gactcgttag ctgaatcgtg
    27961 taagaaaaaa tagaattcat aaattgcgtc aataatttcc aaacgctaag ccgcacaaaa
    28021 gaacaaatat tattaagcga tactgatgtt tatttagccg taataattac ggcgagtgat
    28081 tactacagct aaataatatt tacagattac gtcagatgcg tttttcgtag agccagataa
    28141 tccagcaaac caccaagcac gacgccagaa agggcgacta aaaccccgac ttccagcaga
    28201 gcaggcagga aagagaagtg cgtcaaatcc agcgcgtcca tcgtcaacag cagtaaccac
    28261 acggccaaca ggctgacgcc cgccgccagt gtccacatgg caaaggcata acctgccgga
    28321 tattcggtac gggagataaa gttgtcattt actcgtgtat attcaagcgt ctggcggcaa
    28381 accagcagca aaataagccc cggtacggca gcggcgacgg agaatagata gaatgtcgac
    28441 cagccgtgtg cttcaacaaa ccaacccgcc acggggccga cataaactcg tcctacagcg
    28501 gaaagcgctg agagcagggc aaattgagta gcggaaaatg acttattaca cagcgtcatt
    28561 aacagcgcga caaaggctga tgtgcccatc ccgccacaga ggttttcgaa aaagacggct
    28621 gcgcccatgc tgtagagatg cttatcagta atcgacagca gccagtaacc agcgttagac
    28681 gcaccttgta aaatgccgaa aatcagcagc gcccggaaca gcgacaggcg ctgcatcaaa
    28741 atcccaccgt acaatgcgcc cacaatggtc gccagtaagc caagcgtttt gttaaccacg
    28801 cctacttcac ccgcatcaaa cccgacgccg cgaatcaaaa acgtggttgt caggctcatg
    28861 gcgaatgcgt cgcccagctt atacagcacg ataagaagca aaataagcca ggcattattg
    28921 cgaccaaaga aatctcgcag aggtgcaaca accgcttgtt ccagcgtttt tggcacagga
    28981 atggtgtcgg tcggttctgg tgcaagcaac gtcgcgataa tacaagggat caacagcgcc
    29041 gccatcaacc agtacatgcc ctgccagccc agccatttat ctgccagcca cagggctaag
    29101 ccgccggaaa ccagcatccc taaacggtaa cccagcacgc tgattgccgc gcccgcacca
    29161 cgttcttctg ccgggagcac atcggttttc cacgcatcga agacaatatc ctgggaggcc
    29221 gagcaaaaag cgatcaccac cgccagtgcc gccatccagc ggagttgggt gccaggttcg
    29281 agaaaaccca tcgccgcaat ggcgactaat aacaggattt gcgtggcgag cagccaaccg
    29341 cgccgccgcc caaaaaatgg gggcgtgtag cggtccatca gcggtgacca gaggaattta
    29401 aaaacgtaag cctggcctac cagagagaag aaaccaatgg ttttgagatc aatattctcg
    29461 accgtcatcc aagcctgtaa ggtaccggat gtcagggcga gcggtagccc ggaagcaaaa
    29521 cccaggatca gcaatatggc tgaacgcggc tgttgaaaaa tacgtaaata ttgactggac
    29581 atgggcgtct acaggcccgg tttgcaccgg gccagagggc agaaattaac gcgcgttctg
    29641 cttgatgaat tcgtgaatgc tggtgtcctg agacatatca gcgatggtat cggtcagcac
    29701 actgttaacc gcatcggcga tatttttgtt ggaggcctgg aacgcacctt caacgttgta
    29761 gctggcacga tagtttttgg ttattttgtt gccattctgc gcggtagcga tgatggcgat
    29821 atccgctttg gtagcgatgt tgtagcgcac gttgccctgg gacacgtcag catacagttg
    29881 gctaacgatg atttgcagat taaccgggcc attcggacca accatgtaac cacgcgcggt
    29941 catctgtttt tccagcactt cttgcagcag gaaacgcaga tcgcgggagg cggccagggt
    30001 aacgatttga ttatcgcggg tgacttttgc cagcgcctga tcggtacgct gatcggcacc
    30061 attaatgctt acggtgacgc ccatcaggct tggatcctgc tgtggcagtg taatcgtcgg
    30121 ggaaacttca atagttgttg gcggttttgc gcatcctgca agcataaaca gagcaactaa
    30181 cgggaagagg atttttttga acatgttcgg gctctcagag actcttaagc gtgtttggta
    30241 aaaattcccg ccatcataac attgccaacg gcgaggggaa gtgggtaagg catgtaaatt
    30301 catcatgttg acgaaataat cacccctggt aaaagaaacg ctgatgcgag gcctgtgttt
    30361 caatctttaa atcagtaaac ttcatacgct tgacggaaaa accaggacga aacctaaata
    30421 tttgttgtta agctgcaatg gaaacggtaa aagcggctag tatttaaagg gatggatgac
    30481 atctcagcgt tgtcggagga gatatttcat gatgatacgt gagcggatag aagaaaaatt
    30541 aagggcggcg ttccaacccg tattcctcga agtagtggat gaaagctatc gtcacaatgt
    30601 cccagccggc tctgaaagcc attttaaagt tgtgctggtc agcgatcgtt ttacgggtga
    30661 acgttttctg aatcgtcatc gaatgattta cagtacttta gcggaggaac tctctactac
    30721 cgttcatgcg ctggctctgc atacttacac tattaaggag tgggaagggt tgcaggacac
    30781 cgtctttgcc tctcctccct gtcgtggagc aggaagcatc gcgtaaaaac gcatttgcaa
    30841 ctgtcggcgc tttttcagta tgttgctaaa gattttatga aaaacggcct gcgggccgtt
    30901 ttgttttgtc tggattttgc gctttttgcc cagcattcag acgaaaattg cccgggaatt
    30961 gtgaaaaaat acgcgacagc gcgcaataac cgttctcgac tcataaaagt gatgccgcta
    31021 taatgccgcg tcctatttga atgctttcgg gatgattctg gtaacaggga atgtgattga
    31081 ttataagaac atcccggttc cgcgaagcca acaacctgtg cttgcggggt aagagttgac
    31141 cgagcactgt gattttttga ggtaacaaga tgcaagtttc agttgaaacc actcaaggcc
    31201 ttggccgccg tgtaacgatt actatcgctg ctgacagcat cgagaccgct gttaaaagcg
    31261 agctggtcaa cgttgcgaaa aaagtacgta ttgacggctt ccgcaaaggc aaagtgccaa
    31321 tgaatatcgt tgctcagcgt tatggcgcgt ctgtacgcca ggacgttctg ggtgacctga
    31381 tgagccgtaa cttcattgac gccatcatta aagaaaaaat caatccggct ggcgcaccga
    31441 cttatgttcc gggcgaatac aagctgggtg aagacttcac ttactctgta gagtttgaag
    31501 tttatccgga agttgaactg cagggtctgg aagcgatcga agttgaaaaa ccgatcgttg
    31561 aagtgaccga cgctgacgtt gacggcatgc tggatactct gcgtaaacag caggcgacct
    31621 ggaaagaaaa agacagcgct gttgaagcag aagaccgcgt aaccatcgac ttcaccggtt
    31681 ctgtagacag cgaagagttc gaaggcggta aagcgtctga tttcgtactg gcgatgggcc
    31741 agggtcgtat gatcccgggc tttgaagacg gtatcaaagg ccacaaagct ggcgaagagt
    31801 tcaccatcga cgtgaccttc ccggaagaat accacgcaga aaacctgaaa ggtaaagcag
    31861 cgaaattcgc tatcaacctg aagaaagttg aagagcgtga actgccggaa ctgactgcag
    31921 aattcatcaa acgtttcggc gttgaagatg gttccgtaga aggtctgcgc gctgaagtgc
    31981 gtaaaaacat ggagcgcgag ctgaagagcg ccatccgtaa ccgcgttaag tctcaggcga
    32041 tcgaaggtct ggtaaaagct aacgacatcg acgtaccggc tgcgctgatc gacagcgaaa
    32101 tcgacgttct gcgtcgccag gctgcacagc gtttcggtgg caacgaaaaa caagctctgg
    32161 aactgccgcg cgaactgttc gaagaacagg ctaaacgccg cgtagttgtt ggcctgctgc
    32221 tgggcgaagt tatccgcacc aacgagctga aagctgacga agagcgcgtg aaaggcctga
    32281 tcgaagagat ggcttctgcg tacgaagatc cgaaagaagt tatcgagttc tacagcaaaa
    32341 acaaagaact gatggacaac atgcgcaatg ttgctctgga agaacaggct gttgaagctg
    32401 tactggcgaa agcgaaagtg actgaaaaag aaaccacttt caacgagctg atgaaccagc
    32461 aggcgtaatt tacgcagcat aacgcgctaa attcgcacaa aggcccgtca ccgccaggtg
    32521 gtgggctttt tttgtcatga attttgcatg gaaccgtgcg aaaagcctct ttcggtgtta
    32581 gcgtaacaac aaaagattgt tatgcttgaa atatggtgat gccgtaccca taacacaggg
    32641 actagctgat aatccgtcca taaggttaca atcggtacag caggtttttt caattttatc
    32701 caggagacgg aaatgtcata cagcggcgaa cgagataact ttgcacccca tatggcgctg
    32761 gtgccgatgg tcattgaaca gacctcacgc ggtgagcgct cttttgatat ctattctcgt
    32821 ctacttaagg aacgcgtcat ttttctgact ggccaggttg aagaccacat ggctaacctg
    32881 attgtggcgc agatgctgtt cctggaagcg gaaaacccag aaaaagatat ctatctgtac
    32941 attaactccc caggcggggt gatcactgcc gggatgtcta tctatgacac catgcagttt
    33001 atcaagcctg atgtcagcac catctgtatg ggccaggcgg cctcgatggg cgctttcttg
    33061 ctgaccgcag gggcaaaagg taaacgtttt tgcctgccga attcgcgcgt gatgattcac
    33121 caaccgttgg gcggctacca gggccaggcg accgatatcg aaattcatgc ccgtgaaatt
    33181 ctgaaagtta aagggcgcat gaatgaactt atggcgcttc atacgggtca atcattagaa
    33241 cagattgaac gtgataccga gcgcgatcgc ttcctttccg cccctgaagc ggtggaatac
    33301 ggtctggtcg attcgattct gacccatcgt aattgatgcc agaggcgcaa ctgtgccgct
    33361 atacttatcc agggcggcac aacgctgtaa gcggcttgcg cctgagaatg gcatttgcgt
    33421 cgtcgtgtgc ggcacaaaga acaaagaaga ggttttgacc catgacagat aaacgcaaag
    33481 atggctcagg caaattgctg tattgctctt tttgcggcaa aagccagcat gaagtgcgca
    33541 agctgattgc cggtccatcc gtgtatatct gcgacgaatg tgttgattta tgtaacgaca
    33601 tcattcgcga agagattaaa gaagttgcac cgcatcgtga acgcagtgcg ctaccgacgc
    33661 cgcatgaaat tcgcaaccac ctggacgatt acgttatcgg ccaggaacag gcgaaaaaag
    33721 tgctggcggt cgcggtatac aaccattaca aacgtctgcg caacggcgat accagcaatg
    33781 gtgtcgagtt gggcaaaagt aacattctgc tgatcggtcc gaccggttcc ggtaaaacgc
    33841 tgctggctga aacgctggcg cgcctgctgg atgttccgtt caccatggcc gacgcgacta
    33901 cactgaccga agccggttat gtgggtgaag acgttgaaaa catcattcag aagctgttgc
    33961 agaaatgcga ctacgatgtc cagaaagcac agcgtggtat tgtctacatc gatgaaatcg
    34021 acaagatttc tcgtaagtcg gacaacccgt ctattacccg tgacgtttcc ggtgaaggcg
    34081 tacagcaggc actgttgaaa ctgatcgaag gtactgttgc tgccgttcca ccgcagggcg
    34141 ggcgtaagca tccgcagcag gaatttttgc aggttgatac ctccaagatc ctgtttattt
    34201 gtggcggtgc gtttgccggt ctggataaag tgatttccca ccgtgtagaa accggctccg
    34261 gcattggttt tggcgcgacg gtaaaagcga agtccgacaa agcaagcgaa ggcgagctgc
    34321 tggcgcaggt tgaaccggaa gatctgatca agtttggtct tatccctgag tttattggtc
    34381 gtctgccggt tgtcgcaacg ttgaatgaac tgagcgaaga agctctgatt cagatcctca
    34441 aagagccgaa aaacgccctg accaagcagt atcaggcgct gtttaatctg gaaggcgtgg
    34501 atctggaatt ccgtgacgag gcgctggatg ctatcgctaa gaaagcgatg gcgcgtaaaa
    34561 ccggtgcccg tggcctgcgt tccatcgtag aagccgcact gctcgatacc atgtacgatc
    34621 tgccgtccat ggaagatgtc gaaaaagtgg ttatcgacga gtcggtaatt gatggtcaaa
    34681 gcaaaccgtt gctgatttat ggtaagccgg aagcgcaaca ggcatctggt gaataattaa
    34741 ccattcccat acaattagtt aaccaaaaag gggggatttt atctcccctt taatttttcc
    34801 tctattctcg gcgttgaatg tgggggaaac atccccatat actgacgtac atgttaatag
    34861 atggcgtgaa gcacagtcgt gtcatctgat tacctggcgg aaattaaact aagagagagc
    34921 tctatgaatc ctgagcgttc tgaacgcatt gaaatccccg tattgccgct gcgcgatgtg
    34981 gtggtttatc cgcacatggt cattccctta tttgtcgggc gggaaaaatc tatccgttgt
    35041 ctggaagcgg cgatggacca tgataaaaaa attatgctgg tcgcgcagaa agaagcttca
    35101 acggatgagc cgggtgtaaa cgatcttttc accgtcggga ccgtggcctc tatattgcag
    35161 atgctgaaac tgcctgacgg caccgtcaaa gtgctggtcg aggggttaca gcgcgcgcgt
    35221 atttctgcgc tctctgacaa tggcgaacac ttttctgcga aggcggagta tctggagtcg
    35281 ccgaccattg atgagcggga acaggaagtg ctggtgcgta ctgcaatcag ccagttcgaa
    35341 ggctacatca agctgaacaa aaaaatccca ccagaagtgc tgacgtcgct gaatagcatc
    35401 gacgatccgg cgcgtctggc ggataccatt gctgcacata tgccactgaa actggctgac
    35461 aaacagtccg ttctggagat gtccgacgtt aacgaacgtc tggaatatct gatggcaatg
    35521 atggaatcgg aaatcgatct gctgcaggtt gagaaacgca ttcgcaaccg cgttaaaaag
    35581 cagatggaga aatcccagcg tgagtactat ctgaacgagc aaatgaaagc tattcagaaa
    35641 gaactcggtg aaatggacga cgcgccggac gaaaacgaag ccctgaagcg caaaatcgac
    35701 gcggcgaaga tgccgaaaga ggcaaaagag aaagcggaag cagagttgca gaagctgaaa
    35761 atgatgtctc cgatgtcggc agaagcgacc gtagtgcgtg gttatatcga ctggatggta
    35821 caggtaccgt ggaatgcgcg cagcaaggtc aaaaaagacc tgcgtcaggc gcaggaaatc
    35881 cttgataccg accattatgg tctggagcgc gtgaaagatc gcatccttga gtaccttgcg
    35941 gttcaaagcc gtgtcaacaa aatcaaggga ccgatcctct gcctggtagg gccgccgggg
    36001 gtaggtaaaa cctctcttgg tcagtccatt gccaaagcca ccgggcgtaa atatgtccgt
    36061 atggcgctgg gcggcgtgcg tgatgaagcg gaaatccgtg gtcaccgccg tacttacatc
    36121 ggttctatgc cgggtaaact gatccagaaa atggcgaaag tgggcgtgaa aaacccgctg
    36181 ttcctgctcg atgagatcga caaaatgtct tctgacatgc gaggcgatcc ggcctctgca
    36241 ctgcttgaag tgctggatcc agagcagaac gtagcgttca gcgaccacta cctggaagtg
    36301 gattacgatc tcagcgacgt gatgtttgtc gcgacgtcga actccatgaa cattccggca
    36361 ccgctgctgg atcgtatgga agtgattcgc ctctccggtt ataccgaaga tgaaaaactg
    36421 aacatcgcca aacgtcacct gctgccgaag cagattgaac gtaatgcact gaaaaaaggt
    36481 gagctgaccg tcgacgatag cgccattatc ggcattattc gttactacac ccgtgaggcg
    36541 ggcgtgcgtg gtctggagcg tgaaatctcc aaactgtgcc gcaaagcggt taagcagtta
    36601 ctgctcgata agtcattaaa acatatcgaa attaacggcg ataacctgca tgactacctc
    36661 ggtgttcagc gtttcgacta tggtcgcgct gataacgaaa actgtgtcgg tcaggtaacc
    36721 ggtctggcgt ggacggaagt gggcggtgac ttgctgacca ttgaaaccgc atgtgttccg
    36781 ggtaaaggca aactgaccta taccggttcg ctcggcgaag tgatgcagga gtctattcag
    36841 gcggcgttaa cggtggttcg tgcgcgtgcg gaaaaactgg ggatcaaccc tgatttttac
    36901 gaaaaacgtg acatccacgt ccacgtaccg gaaggtgcga cgccgaaaga tggtccgagt
    36961 gccggtattg ctatgtgcac cgcgctggtt tcttgcctga ccggtaaccc ggttcgtgcc
    37021 gatgtggcaa tgaccggtga gatcactctg cgtggtcagg tactgccgat cggtggtttg
    37081 aaagaaaaac tcctggcagc gcatcgcggc gggattaaaa cagtgctaat tccgttcgaa
    37141 aataaacgcg atctggaaga gattcctgac aacgtaattg ccgatctgga cattcatcct
    37201 gtgaagcgca ttgaggaagt tctgactctg gcgctgcaaa atgaaccgtc tggcatgcag
    37261 gttgtgactg caaaatagtg acctcgcgca aaatgcacta ataaaaacag ggctggcagg
    37321 ctaattcggg cttgccagcc tttttttgtc tcgctaagtt agatggcgga tcgggcttgc
    37381 ccttattaag gggtgttgta aggggatggc tggcctgata taactgctgc gcgttcgtac
    37441 cttgaaggat tcaagtgcga tataaattat aaagaggaag agaagagtga ataaatctca
    37501 attgatcgac aagattgctg caggggctga tatctctaaa gctgcggctg gccgtgcgtt
    37561 agatgctatt attgcttccg taactgaatc tctgaaagaa ggggatgatg tagcactggt
    37621 aggttttggt acttttgccg ttaaagagcg tgctgcccgt actggccgca acccgcagac
    37681 cggtaaagag atcaccatcg ctgctgctaa agtaccgagc ttccgtgcag gtaaagcact
    37741 gaaagacgcg gtaaactaag cgttgtcccc ggtggggatg tgacgaagtt caagggcgca
    37801 tctactgatg tgcctttttt atttgtattc ggtgactttc tgcgtcttgt gggctgacaa
    37861 ttgcccccgt ttcttgtcac aataggcctt tgcgcgcatc gatacgttgc gtgaggtaca
    37921 cagtcatcta cagcggagtg ttgttacacc atgatggaca gcttacgcac ggctgcaaac
    37981 agtctcgtgc tcaagattat tttcggtatc attatcgtgt cgttcatatt gaccggcgtg
    38041 agtggttacc tgattggcgg aggcaataac tacgccgcaa aagtgaatga ccaggaaatc
    38101 agccgtgggc agttcgagaa cgcctttaac agcgagcgta atcgcatgca gcaacagctg
    38161 ggcgatcaat actccgagct ggcagcgaat gaaggctata tgaaaaccct gcgtcaacag
    38221 gtgctgaatc gtctgatcga cgaggcgctg ttggatcagt acgctcgtga gctgaaactg
    38281 ggtatcagtg atgagcaggt taaacaggcg attttcgcga ccccagcctt ccaggttgac
    38341 ggcaaatttg ataacagccg ctataacggt atcctcaacc agatggggat gaccgccgat
    38401 cagtacgccc aggcgctgcg taaccagctc actacccaac agctgattaa cggtgttgcc
    38461 ggtaccgatt ttatgctgaa aggtgaaacc gacgagctgg cggcactggt cgcgcaacaa
    38521 cgcgtggtgc gtgaggcgac tatcgatgtt aacgcgctgg cggcgaagca gcctgtgacc
    38581 gaacaggaga ttgccagcta ctacgaacaa aacaaaaaca atttcatgac gccggaacaa
    38641 ttccgcgtga gttacatcaa gctggatgcc gcaacgatgc agcaaccggt tagcgatgcg
    38701 gatatccaga gctactacga tcagcatcag gatcaattca cccagccgca gcgtacccgc
    38761 tacagcatca tccagaccaa aactgaagat gaagcgaaag cggtacttga tgagctgaat
    38821 aaaggcggtg attttgctgc attagccaaa gaaaaatctg ccgatattat ctctgcccgt
    38881 aacggcggcg atatgggttg gttagaagat gccactatcc ccgacgaact gaaaaatgct
    38941 ggtctgaaag aaaaaggcca actgtctggt gtcatcaaat cttcggtcgg tttcctgatt
    39001 gtacgtctgg acgacattca gccagcgaaa gtgaaatcgt tagacgaagt acgtgacgac
    39061 attgcggcga aagtgaaaca cgaaaaagcc ctcgatgcgt actatgcgct gcagcagaaa
    39121 gtgagcgatg cggcaagcaa cgacaccgag tctctggccg gtgcagagca agctgccggc
    39181 gttaaagcca ctcagacggg ttggttcagc aaagataacc tgccggaaga gttgaacttc
    39241 aagccggttg ccgacgctat ctttaacggc ggtctggtag gtgaaaacgg cgcgccgggc
    39301 atcaactctg acatcatcac cgtagacggc gaccgtgcat tcgtgctgcg catcagcgag
    39361 cacaaaccgg aagcggtgaa accgttggca gatgttcagg aacaagttaa ggcactggtt
    39421 cagcacaaca aagctgaaca acaggcgaaa gtggatgctg agaaactgct ggttgatttg
    39481 aaagccggca aaggtgcgga agctatgcag gctgccggtc tgaaatttgg cgagccgaaa
    39541 accttaagcc gttccggtcg tgacccgatt agccaggcgg cgtttgcact gccactgcca
    39601 gcgaaagaca aaccgagcta cggtatggcg accgatatgc aaggtaatgt ggttctgctg
    39661 gcgctggatg aagtgaaaca aggttcaatg ccggaagatc agaaaaaagc gatggtgcag
    39721 ggtatcaccc agaacaacgc acaaatcgtc tttgaagctc tgatgagtaa cctgcgtaaa
    39781 gaggcgaaaa tcaaaattgg cgatgcgctg gaacagcaat aatcctgaag ccgcctcgca
    39841 aaaaaatgcg ttgcagctgt aacaagaaaa ggtcgctttc gcggcctttt ccatttctga
    39901 acattgccat ttgtttactg ttttcactgc cgttaaggtg attccactgt taacaaacaa
    39961 ggagaaaaca gtatgaaaca cggaattaaa gcactgctca ttaccctgtc cctggcctgt
    40021 gccggaatgt ctcatagcgc gctggcggca gcttctgtgg cgaaaccgac ggcggtagaa
    40081 accaaagcgg aagctcctgc agcacaaagt aaagcagcag taccggcgaa agccagtgac
    40141 gaagaaggca cccgggtcag cattaataat gccagcgcgg aagagctagc ccgcgcgatg
    40201 aatggcgttg gcctgaagaa agcgcaggcg attgtcagtt atcgcgaaga gtacggtccg
    40261 tttaaaacag tggaggatct aaagcaggtg ccggggatgg gcaattcgct ggtggaacgt
    40321 aatctggcgg tattaaccct gtaattaatt tgcatagtgg caattttttg ccagactgaa
    40381 gaggtcatac cagttatgac ctctgtactt ataacaacaa cgtaaggtta ttgcgctatg
    40441 caaacacaaa taaaagttcg tggatatcat ctcgacgttt accagcacgt caacaacgcc
    40501 cgctaccttg aatttctcga agaagcccgc tgggatgggt tggaaaatag cgacagtttt
    40561 cagtggatga cggcccataa catcgccttc gtcgtggtca atatcaatat taactatcgt
    40621 cgcccagcgg tattaagtga cctgttaact attaccagtc agttgcagca attaaacggt
    40681 aaaagcggca tcttaagcca ggtcattaca ctggagccgg aagggcaggt ggtagcggat
    40741 gcgcttatta cgtttgtttg tattgatctt aaaacgcaga aagcattagc tctggaaggg
    40801 gaattgcgcg aaaagctgga gcagatggtt aagtaaacgt tttgtggtgc cggatgctca
    40861 agccgcatcc ggcgacaccc ggaataatta cctcaacccg gttttctgct tcatcgctgc
    40921 catcaccgtc ggtttatcgg ccagataatg attcaaaccg ttggcgcgta aattacatgc
    40981 cgcacaatga ccgcaaccgt cgcctttaat gccgttatag caggtcaacg tttcgttacg
    41041 gactaaatcc agtttgccgt aataatctgc cagcgcccag gtttccgctt tatcaatcca
    41101 catcagcggc gtttcaaaac gaatatcttt ctccatgccc aaactgacgg catggtttag
    41161 tgctttcaca aactcatcgc ggcaatccgg gtagccggag aaatccgttt cacagacgcc
    41221 ggtaattacg gcttctgctt ttacctgata cgcatatatt gccgccagcg tcaggaacaa
    41281 aatattacgc cctgggacaa acgtattcgg gatgccatcg gcttcaggtt cataatcagg
    41341 caccggaatg ctgtcacgtg tcaggctgct gaccgccagc tcgttgagca gcgttacatc
    41401 aagaacctta tgcgcgcgtg ctcccagttt cagcgccagt tcgcgtgcca cgtcgatttc
    41461 tgcgcgatgt cgctgaccgt aatcgaacgt cacgcaatgg acttcatcat attgttgtaa
    41521 tgcctgcacc agacaggtgg tggaatcctg gcctccactg aacacaacga cagcacgttt
    41581 catagataat cctgcttgaa caataaaagc gttatggtaa cgcctgcgat taacccggac
    41641 cagctattca ttgcgacggc gcgggaagcc aggcttcggt aaattcaaac cagccgcgcg
    41701 gtgtcagtcg cacaccgttc acgccgggag gggcactaat gcgatagtga tagttgaaca
    41761 gcggcgtcag cgtcgcgtct gccattaact ggctaaaaac cgctttcagg gcattaaatc
    41821 ggttttcctc atcaggcatt acttgcaccg cgtccagcgt cgattgcaga tgtgcatatg
    41881 ctggagcgtc gaaaacatgt ggccatagtg gatcgcaacg tagccattgc tccagagtat
    41941 attccggtgc ttcgccaatt aatctgtcgc ccatcatgag gtctgcgtgt gccagtaggg
    42001 tcgtgtcgtc ccagtttttt gcgttatgaa aaataattgt gagttcacag ccttccgctg
    42061 ccagtgtcgc ctgtaggcgt tctgccatgg tatgaagttc tatcggtagg tgataaacca
    42121 gcgtcaaggt tttcggtagt ttgacttcat ccggtacttg ccagtgcgga atagtccagc
    42181 ctggcagtaa tgcatgactg gcggtgatca ggttttctcc gacttctaac gtttgtaata
    42241 aaccggattg atgaataatg gagatcactt ttcgcgcctg ccagagggag agtcggggac
    42301 ttttgcgcaa cgttaaatag caaaaaccta aactgatgcc actactgacc tggctgaccc
    42361 gttgcagctc ctccggtttg ccgatggtga tttgcacggg atgccgacaa ctggttccca
    42421 aatctttttc gaaaagcggc ggagttatcc agtactcaac cgctttaagc agcggatgac
    42481 gcaaatggta ataatcatgg ctctccaggc gcaccagctc cgctgtgaat tgtgttaagc
    42541 gaaaaggacc agtgccgatc agtgggaatt gcggatgcgc cagatggctg caatagctcg
    42601 ccagccggtg cgcaagccag taatcggggc gatgtaaaaa gaaggtcaga cactgcggat
    42661 gggtgacttc aatacgcttc acgctaataa ataattgatc cagtgctggc agttgtaaca
    42721 gcatcaataa tcgctggtgt aagtgtgagg cttttactgc atcgccgtta tgccagtgta
    42781 gggttgaacg aagataaaag tcccagcgta acccgtcagt agaggtttcc cagtgatgcg
    42841 ctaaatcgcc aatcgggcgc tgagtattat tatcgaagcg ggtcaggccg gaaaatatct
    42901 gcccggcgag atgctgctcg gcacggccgg gcaaaaagcc tggttgtagc ggttcgagcg
    42961 ggcgatagta gggaatacgc aacgtgggtg tatcgttttg ccattgtccg cccataaacg
    43021 gctgtaacag agtgcgtagc tcacctaggg ccagttgcgc cagctccagc acatcttgct
    43081 gctttccggt ttccagtgcc tgttccatca tcgcattgcg tagcgattcc ggcgtgacca
    43141 gaaagcgtaa ttgtccgcgc tttccgcgtc ctgactgcgc ctgccactcc agccatcccg
    43201 cctcctgtgc ctgacgcaac agcgtacgaa catggcgttc gctgcaaaaa cagcgttcgg
    43261 ccagttcgct gacggtgacg gtttgcggct ttccggctga aggttgccac agacgttgat
    43321 actggttaag acggttgagc aatcgcatat aaacccggaa caatattatt taactattca
    43381 ctattacttc cgtatatatc aggtgatact caatctccat taaccgtgtc acagagtgga
    43441 gaaagaaatg gctcgtctgg cagcatttga tatggatggc actttattga tgcctgacca
    43501 tcatttaggt gagaaaaccc tctctacttt ggcgcgactg cgtgaacgcg acattaccct
    43561 cacttttgcc acggggcgtc atgcgctgga gatgcagcat attctcgggg cgctatcgct
    43621 ggatgcatat ttgattaccg gcaacggaac gcgcgtgcat tctctggaag gtgaactttt
    43681 acatcgtgat gatttacctg cggatgtcgc ggagctggtg ctgtatcagc aatgggatac
    43741 ccgagccagc atgcatatct tcaatgacga cggttggttt accgggaaag agatccctgc
    43801 gttgttgcag gcatttgtct atagcggttt tcgttatcag ataatcgatg tcaaaaaaat
    43861 gccactcggc agcgtcacca agatctgctt ctgtggcgat cacgacgatc ttacacgctt
    43921 gcagatccag ctatacgaag cattaggcga gcgtgcacat ttgtgttttt ccgccacgga
    43981 ttgcctcgaa gtgctgccgg tgggctgcaa taaaggcgct gcattgacgg tgctgaccca
    44041 acatctaggt ttatcgttgc gcgattgcat ggcctttggt gatgcgatga acgatcgcga
    44101 aatgttaggc agcgtcggta gcgggtttat tatgggcaat gcgatgccgc aactgcgcgc
    44161 ggagctcccg catttaccgg tgattggaca ttgccgaaat taggctgtct ctcactattt
    44221 gacgcactga ctggactatc cacatctacc ttattccccc gaataatgag atcccttcca
    44281 gcaccgggca attgcccggt tttttttgcg ttgaatttgt cattttgtgc cgtggtgttt
    44341 aaaccgcaca gaataaattg tcgtgatttc acctttaaaa tagaattaaa agagaaaaaa
    44401 attctctgtg gaagggctat gttagataaa attgaccgta agctgctggc cttactgcag
    44461 caggattgca ccctctcttt gcaggcactg gctgaagccg ttaatctgac aaccacccct
    44521 tgctggaagc gcctgaaacg gctggaggac gacggtatcc ttatcggcaa agtcgccctg
    44581 ctggatccgg aaaaaatagg cctcggcctg accgcctttg tgctgataaa aacgcaacat
    44641 cacagtagcg aatggtattg ccgctttgtc acggtggtta ccgaaatgcc agaagtgctg
    44701 gggttctggc gcatggctgg tgaatacgat tatctgatgc gcgtccaggt tgccgacatg
    44761 aaacgctacg acgagtttta taagcgtctg gtaaacagcg tgccggggct gtcggacgtc
    44821 acttccagct tcgcgatgga acagattaaa tacaccactt ctttacccat cgaataaata
    44881 tccagaatca ggtcaggaca caacgcgtgc gattatttgc tcaattaagc tggtatttcc
    44941 gtcgggaatg gcgtcgctat ctcggggctg tcgccttgct tgtcattatc gcgatgctgc
    45001 aactggttcc gccaaaagtg gttggtattg ttgtcgatgg cgtgacagaa caacacttta
    45061 ctaccgggca gatcctgatg tggatcgcca ccatggtgct gattgccgtt gtggtttatt
    45121 tcctgcgtta cgtctggcgg gtattgctgt ttggtgcgtc ttatcaactg gctgttgaac
    45181 tgcgtgaaga ttattaccgt cagctaagcc ggcagcatcc tgagttttac ctgcgtcatc
    45241 gcacgggtga cctcatggct cgtgcgacca atgacgtcga tcgcgtcgtg tttgcagccg
    45301 gagaaggggt gctgacgctg gtggattcac tggtgatggg ctgcgctgtg ttgattatga
    45361 tgtctacgca aattagctgg cagttgacct tatttgccct gttgccgatg ccagtgatgg
    45421 cgatcatgat caagcgcaac ggcgatgcct tgcatgaacg ctttaagctg gcacaggcgg
    45481 cgttttctag tcttaatgac cgcacccagg aaagcctcac cagtatcagc atgatcaaag
    45541 cctttggtct ggaagatcgc cagtcggcgt tatttgccgc ggatgccgaa gataccggca
    45601 aaaaaaacat gcgggtggcg cgtattgatg ctcgtttcga cccgaccatc tatatcgcga
    45661 ttggtatggc gaatttgctg gcgattggcg gcggtagttg gatggtggtg cagggcagtt
    45721 taacgctggg ccagctcacc agttttatga tgtatttagg tctgatgatt tggccaatgc
    45781 tggcgctggc atggatgttt aacattgtgg aacgtggtag tgctgcatac agccgtattc
    45841 gcgcgatgct ggcggaagcg ccggtggtga acgatggtag cgaacccgtg ccggaagggc
    45901 gtggcgaact ggatgtaaat attcaccagt tcacgtatcc gcagactgac catcctgcgc
    45961 tggaaaacgt caatttcgcc ctgaaacccg gtcagatgct gggtatctgc gggccgactg
    46021 gttccggcaa aagtaccctg ttgtcgctca ttcagcgtca tttcgacgtc agcgaggggg
    46081 atattcgctt tcatgatatt cctctgacga agttacaact cgatagctgg cgtagccgcc
    46141 tggcggtagt tagccagaca ccgttccttt tttccgacac cgtagcgaac aacatcgcgt
    46201 tgggttgccc gaatgccacc cagcaagaga ttgagcatgt cgcgcggtta gccagcgtac
    46261 atgacgatat tttgcgtcta ccgcaaggtt acgatacaga agtgggcgag cgcggcgtga
    46321 tgctttccgg tgggcaaaaa cagcgtatct ccattgctcg agcgttatta gtcaacgcgg
    46381 aaatcctcat ccttgatgat gcgctttcgg cggtggacgg acgcacagag caccagatcc
    46441 tgcataacct gcgtcagtgg ggacagggaa gaacggtaat catcagtgcc catcgccttt
    46501 cggcactgac ggaagccagt gaaaatattg tgatgcagca cggacatatc gcccagcgtg
    46561 gcaatcatga tgagctggcg caacaaagcg gctggtatcg cgatatgtat cgctatcaac
    46621 aactggaggc ggcgctcgac gacgctccgg aaattcgcga ggaggccgtc gatgcgtagt
    46681 tttagccaac tgtggccgac tctcaagcgt ctgttagcgt acggttcgcc gtggcgtaaa
    46741 ccgctgggga ttgcggtcct gatgatgtgg gttgcggcag cggcagaagt cagtgggccg
    46801 ctgcttatca gctattttat cgacaatatg gtagcgaaaa ataacctgcc gttgaaaatg
    46861 gttgcagggc tggctgcggc gtatgttggg ctacaactgt ttgccgccgg gctacattac
    46921 gcgcagtcgc tgctgtttaa tcgggcggca gtaggcgtag tgcaacagtt gcgtaccgac
    46981 gtgatggatg ccgcattgcg ccagccatta agcgagtttg atacccaacc cgtcgggcag
    47041 gtgatttccc gcgtcactaa tgacactgaa gtgatccgcg atctctacgt taccgtagtg
    47101 gcaactgtcc tgcgtagcgc cgcactggtg ggcgcgatgc tggtggcgat gttcagcctc
    47161 gactggcgaa tggcgctggt ggcgataatg attttcccgg tggtgctggt ggtaatggtg
    47221 atataccagc gttacagcac gccgattgtc cgtcgtgtgc gcgcctatct ggcggatatc
    47281 aacgacggct ttaacgaaat catcaatggc atgagcgtta tccagcagtt ccgtcagcag
    47341 gcgcgatttg gcgaacgtat gggggaggcc agtcgttcac actatatggc caggatgcaa
    47401 accctgcgcc tcgacggttt tctgctgcgt ccgctgctga gtctgttttc atcgctcatt
    47461 ctttgtggct tgttgatgct gtttggcttc tccgctagcg gcaccattga agtaggcgtg
    47521 ctgtatgcgt ttatcagcta tcttgggcga cttaacgaac ccttaatcga actgaccacg
    47581 caacaggcga tgctgcaaca ggctgttgtt gctggtgagc gcgtgtttga actgatggac
    47641 ggaccgcgcc agcaatatgg caatgatgat cgcccgttac agagtggcac catcgaagtc
    47701 gataacgtgt catttgctta tcgcgatgac aatctggtgc taaagaacat taatctctct
    47761 gtgccttcgc gtaactttgt ggcactggtc gggcataccg gcagtggcaa aagcaccctc
    47821 gccagtttat tgatgggcta ttacccgcta acggaaggtg agattcgcct tgatggtcgt
    47881 ccattaagtt cgctaagtca cagcgcgctg cgccagggcg tggcaatggt gcagcaagat
    47941 ccggtggtgc tggcggatac cttcctcgcc aacgtgacgc tggggcgtga tatctccgaa
    48001 gaacgcgtct ggcaggcgct ggaaaccgtg caactggcgg agctggcgcg cagcatgagt
    48061 gatggtattt atacgccgct gggcgagcag gggaataatc tctccgtcgg gcaaaagcaa
    48121 ctgctggcac tggcacgcgt gttggtggag acgccgcaaa tcctgatcct tgatgaggca
    48181 accgccagca ttgactccgg tactgaacag gcgattcaac atgctctggc ggctgtgcgt
    48241 gaacatacca cgctggtggt gattgctcac cgcttatcga ccattgttga tgccgacacc
    48301 attctggtgc ttcatcgtgg gcaagccgtg gagcagggca ctcaccagca actgctggcg
    48361 gcccagggac gctactggca gatgtatcaa ctgcaacttg cgggcgaaga gctggcagcc
    48421 agcgtgcgtg aagaagaatc attgagcgcc tgaatagcgc aatatttcat cgttggtgca
    48481 aaaatgtaac gcactgtgca ctgtcatagt gcgttttcat tttcaatctt cttaacttcc
    48541 tgctctcttt ctcgtttttc atttctggca caccgcttgc aataccttct tcgtgtagca
    48601 gaaccattac cgaattctga ctggagggga tctatgaagc tggtgaccgt gataatcaaa
    48661 ccattcaagc tggaagacgt tcgtgaagcg ttatcttcca ttggtattca gggcctgacc
    48721 gtcaccgaag tgaaaggttt cgggcgtcag aaagggcatg ccgagctgta ccggggggcg
    48781 gaatacagcg tcaacttcct gccaaaagta aaaattgatg tggcaattgc tgatgaccaa
    48841 ctcgatgaag tgatcgatat cgtcagtaag gcggcttaca ccggaaaaat tggcgacggc
    48901 aaaatcttcg tcgctgaatt gcaacgcgtc attcgtattc gtaccggcga agccgacgaa
    48961 gcggcgctgt aatctctggc acacagcaac aggaacgaaa aatgaagata gcgacgataa
    49021 aaactgggct tgcttcactg gcgatgctgc cgggactggt aatggctgca cctgcggtgg
    49081 ccgataaagc cgacaatgcg tttatgatga tttgtactgc gctggtgctg tttatgacta
    49141 ttccggggat tgccctgttt tacggtgggt tgattcgcgg caaaaacgtg ctgtcgatgc
    49201 tgatgcaggt gacggtgacg tttgcactgg tctgtattct ctgggtggtt tacggttact
    49261 cgctggcgtt tggtgagggt aacaacttct tcggcaacgt taactggttg atgctgaaaa
    49321 acatcgaact gacggcggtg atgggcagca tttatcagta tatccacgta gcgtttcagg
    49381 gatcgtttgc ctgcattacc gtaggcttga tagttggggc gctggcggaa cgaatccgct
    49441 tctcagctgt gttgattttc gtggtggtat ggctgacgct ctcttacatt ccgattgcgc
    49501 atatggtgtg gggcggcggt ttgctggctt ctcacggtgc actggatttc gcgggtggca
    49561 cagtggtgca cattaacgcc gcaatcgccg gtctggtggg cgcgtatctg ataggcaaac
    49621 gcgtgggctt cggtaaagag gcgtttaaac cgcacaacct gccgatggtc ttcaccggga
    49681 ctgccattct ctatatcggt tggtttggct ttaacgccgg gtcagcgggc acggcgaatg
    49741 aaatcgcggc actggcattt gtgaatactg tggtcgcaac ggcggcggca attcttggct
    49801 ggatcttcgg tgaatgggcg ctgcgtggta agccttcact gctgggggcg tgttctggcg
    49861 cgattgccgg tctggtcggc gtgacgccag cgtgcggcta cattggggtt ggcggcgcgt
    49921 tgattatcgg cgtggtagct ggtctggcgg gcttgtgggg cgttaccatg ctcaaacgct
    49981 tgctgcgggt ggatgatccc tgcgatgtct tcggtgtgca cggcgtttgt ggcattgtcg
    50041 gctgtatcat gaccgggatt tttgccgcca gctcgctggg cggcgtgggc ttcgctgaag
    50101 gtgtgacgat gggtcatcag ttgctggtac agctggaaag catcgccatt acgatcgtct
    50161 ggtccggtgt ggtggcattt attggctaca aattggcgga tctgacggtt ggtctgcgtg
    50221 taccggaaga gcaggagcta gaagggctgg atgtcaacag tcacggcgag aatgcctata
    50281 acgcgtaaca agcactgcaa aaaacagccg gacggttttc acctccggct atttttttaa
    50341 ttgtgattac gcatcacccc ttcctgaacg gtcgaggcaa ccagtacgcc gtcctgggta
    50401 taaaactcac cgcgcacaaa gccacgtgcg ctggacgccg aggtgctctc cacgctatac
    50461 agcagccatt cattcaaatt aaacgggcga tggaaccaca tggaatggtc aatggtggca
    50521 atctgaatcc ccggttcgag aaaaccgatg ccgtgcggct gtagagctac cggcaggaag
    50581 ttaagatcag aagcgtaacc gagcagatac tgatgaacgc gcaggtcatc cggcacgcta
    50641 ccatttgcgc ggatccacac ctgacgatgt ggttctgcga cgtgaccttt cagtgggtta
    50701 tgaaactcca ccggacggac ttccagcgga cgatcgcaga tgaatttatc tttaagcact
    50761 ggcggcagca ggtgcgccag cgattgggcg atttgcgttt ccgaaggcag gccatcaggc
    50821 gctggcgcgg acggcattgt tttttgatgt tcgaaacccg cttctggtgc ctggaaagag
    50881 gccgtcatat aaaaaatcgg tttgccgttt tgaatagcag caacccggcg ggcgctgaag
    50941 ctgttaccat cacgcagcgt ttcgacatca taaataatcg gtttcttact atcgcctggg
    51001 cgaagaaagt agctgtgaaa cgaatgtacc agccgctctt ccgggacggt ctcttttgca
    51061 gcatacaagg cctgacccac gacctggccg ccaaacacct ggcgtaaacc taaatcttca
    51121 ctctggccgc gaaagagtcc tgcctcaatt ttttccagat ttaacaatgc cagtaaattt
    51181 tttagcgcct gactcatata actctccagt aacaaagctg ccgcagcaag ccaaagtgag
    51241 ttgagtataa cgcaaatttg ccactggtcc gatgggtgca ataatctgaa ttacgggcta
    51301 attacaggca gaaatgcgtg atgtgtgcca cacttattga cgttactatt ttgttaacca
    51361 ctcttccggc gaggaaagtt agcccgctgg tgcattgata ataaggagaa atgaatgaaa
    51421 ctcgtgcaca tggccagtgg tttagcggtt gcgattgcgt tggcggcttg cgcagataaa
    51481 agcgcggata ttcagacgcc agccccggct gcaaatacgt ctatttcagc aacacaacaa
    51541 cctgctatcc agcaaccgaa tgtctccggt accgtctgga tccggcagaa agtcgcactg
    51601 ccgcctgatg ctgtgctgac cgtgacactt tctgacgcgt cgttagccga tgcaccgtca
    51661 aaagtgctgg cgcagaaagc ggtgcgtacc gaaggtaaac agtcaccatt cagctttgtt
    51721 ctgccattta acccggcaga tgttcagccg aacgcgcgta ttctgttgag tgcggcgatt
    51781 accgtgaatg acaaactggt atttattacc gataccgttc agccggtgat caacaagggc
    51841 ggaactaaag ccgacctgac attggtgccg gtgcagcaaa ccgccgtgcc ggttcaggcc
    51901 agcggtggtg caacgactac cgtaccttcg acttcaccaa ctcaggtgaa tccgtcttcg
    51961 gcagttccag ctcctacgca atattaagcg acgttaaccc tctcc
//
LOCUS       DAJTMC010000007        47223 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.007, whole genome shotgun sequence.
ACCESSION   DAJTMC010000007 DAJTMC010000000
VERSION     DAJTMC010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47223
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.007"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            27..764
                     /locus_tag="OO130_000395"
     CDS             27..764
                     /locus_tag="OO130_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HCR5363929.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELRAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGKASTVPAPLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTSWCQ
                     AIAPCHVAWRAINDYDGLSAKKEQNLP"
     gene            875..1885
                     /gene="pyrD"
                     /locus_tag="OO130_000396"
     CDS             875..1885
                     /gene="pyrD"
                     /locus_tag="OO130_000396"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HCR5363930.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            2059..2601
                     /gene="zapC"
                     /locus_tag="OO130_000397"
     CDS             2059..2601
                     /gene="zapC"
                     /locus_tag="OO130_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HCR5363931.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNVLVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(2598..3707)
                     /gene="ycbX"
                     /locus_tag="OO130_000398"
     CDS             complement(2598..3707)
                     /gene="ycbX"
                     /locus_tag="OO130_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HCR5363932.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDRWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     ILEGEVSPLKKSAMGDDGTILCCSCVPKTALKLAR"
     gene            3951..6059
                     /gene="rlmKL"
                     /locus_tag="OO130_000399"
     CDS             3951..6059
                     /gene="rlmKL"
                     /locus_tag="OO130_000399"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HCR5363933.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYSDWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGALSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            6071..7978
                     /locus_tag="OO130_000400"
     CDS             6071..7978
                     /locus_tag="OO130_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5363934.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLLEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIVLIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNIAEGKQEVMVNGKPRHVLSYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKTEEAAAAKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            8108..9361
                     /gene="pqiA"
                     /locus_tag="OO130_000401"
     CDS             8108..9361
                     /gene="pqiA"
                     /locus_tag="OO130_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HCR5363935.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            9366..11006
                     /gene="pqiB"
                     /locus_tag="OO130_000402"
     CDS             9366..11006
                     /gene="pqiB"
                     /locus_tag="OO130_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HCR5363936.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENTDVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            11003..11566
                     /gene="pqiC"
                     /locus_tag="OO130_000403"
     CDS             11003..11566
                     /gene="pqiC"
                     /locus_tag="OO130_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HCR5363937.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSVQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            11822..11989
                     /gene="rmf"
                     /locus_tag="OO130_000404"
     CDS             11822..11989
                     /gene="rmf"
                     /locus_tag="OO130_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HCR5363938.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(12059..12577)
                     /gene="fabA"
                     /locus_tag="OO130_000405"
     CDS             complement(12059..12577)
                     /gene="fabA"
                     /locus_tag="OO130_000405"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HCR5363939.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(12646..14406)
                     /locus_tag="OO130_000406"
     CDS             complement(12646..14406)
                     /locus_tag="OO130_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HCR5363940.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPNLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARTLQSDAGQLVGDHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            14592..15044
                     /gene="matP"
                     /locus_tag="OO130_000407"
     CDS             14592..15044
                     /gene="matP"
                     /locus_tag="OO130_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HCR5363941.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKLDLQALLGKE"
     gene            complement(15120..16160)
                     /gene="ompA"
                     /locus_tag="OO130_000408"
     CDS             complement(15120..16160)
                     /gene="ompA"
                     /locus_tag="OO130_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HCR5363942.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     INNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGSVENGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKSNVYGKNHDTGVSPVFAGGVEYAITPEIATRL
                     EYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKHFTLK
                     SDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLSER
                     RAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEV
                     KGIKDVVTQPQA"
     gene            complement(16517..17026)
                     /gene="sulA"
                     /locus_tag="OO130_000409"
     CDS             complement(16517..17026)
                     /gene="sulA"
                     /locus_tag="OO130_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HCR5363943.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            17245..17874
                     /gene="sxy"
                     /locus_tag="OO130_000410"
     CDS             17245..17874
                     /gene="sxy"
                     /locus_tag="OO130_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HCR5363944.1"
                     /translation="MKSLSYKRIYKSQEYLAMLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(17837..19999)
                     /gene="yccS"
                     /locus_tag="OO130_000411"
     CDS             complement(17837..19999)
                     /gene="yccS"
                     /locus_tag="OO130_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HCR5363945.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGLLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGFWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWQFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNEALASLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(20009..20455)
                     /locus_tag="OO130_000412"
     CDS             complement(20009..20455)
                     /locus_tag="OO130_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HCR5363946.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            20578..22638
                     /gene="helD"
                     /locus_tag="OO130_000413"
     CDS             20578..22638
                     /gene="helD"
                     /locus_tag="OO130_000413"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HCR5363947.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPLPVNRLEEFDNCREAWRK
                     CQAWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLA
                     GAGSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITART
                     FHALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEE
                     MQWSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIK
                     LMAPLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAA
                     LLAALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGERCDLDTTYRFNS
                     RIGKVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERIL
                     ILARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAAR
                     ESIMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPV
                     ARKP"
     gene            complement(22670..23128)
                     /gene="mgsA"
                     /locus_tag="OO130_000414"
     CDS             complement(22670..23128)
                     /gene="mgsA"
                     /locus_tag="OO130_000414"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HCR5363948.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVAANVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(23224..23886)
                     /locus_tag="OO130_000415"
     CDS             complement(23224..23886)
                     /locus_tag="OO130_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HCR5363949.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            24059..24472
                     /locus_tag="OO130_000416"
     CDS             24059..24472
                     /locus_tag="OO130_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HCR5363950.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(24517..24834)
                     /gene="hspQ"
                     /locus_tag="OO130_000417"
     CDS             complement(24517..24834)
                     /gene="hspQ"
                     /locus_tag="OO130_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HCR5363951.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(24892..26082)
                     /gene="rlmI"
                     /locus_tag="OO130_000418"
     CDS             complement(24892..26082)
                     /gene="rlmI"
                     /locus_tag="OO130_000418"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HCR5363952.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALVSALQTLYPECAIYDRSDVA
                     VRKKEGMELTLGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            26177..26455
                     /gene="yccX"
                     /locus_tag="OO130_000419"
     CDS             26177..26455
                     /gene="yccX"
                     /locus_tag="OO130_000419"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HCR5363953.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(26452..26781)
                     /gene="tusE"
                     /locus_tag="OO130_000420"
     CDS             complement(26452..26781)
                     /gene="tusE"
                     /locus_tag="OO130_000420"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HCR5363954.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(26872..27531)
                     /gene="yccA"
                     /locus_tag="OO130_000421"
     CDS             complement(26872..27531)
                     /gene="yccA"
                     /locus_tag="OO130_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HCR5363955.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIVHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(27738..27825)
                     /locus_tag="OO130_000422"
     tRNA            complement(27738..27825)
                     /locus_tag="OO130_000422"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27789..27791),aa:Ser,seq:tga)
     gene            28252..29370
                     /gene="hyaA"
                     /locus_tag="OO130_000423"
     CDS             28252..29370
                     /gene="hyaA"
                     /locus_tag="OO130_000423"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HCR5363956.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            29367..31160
                     /gene="hyaB"
                     /locus_tag="OO130_000424"
     CDS             29367..31160
                     /gene="hyaB"
                     /locus_tag="OO130_000424"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HCR5363957.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMINLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            31179..31886
                     /gene="hyaC"
                     /locus_tag="OO130_000425"
     CDS             31179..31886
                     /gene="hyaC"
                     /locus_tag="OO130_000425"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HCR5363958.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMVFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDGTVISTMV
                     NGYRSHKFGKISNKECS"
     gene            31883..32470
                     /locus_tag="OO130_000426"
     CDS             31883..32470
                     /locus_tag="OO130_000426"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HCR5363959.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLLAAEQSALAQLAAWGSVP
                     QLANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            32467..32865
                     /gene="hyaE"
                     /locus_tag="OO130_000427"
     CDS             32467..32865
                     /gene="hyaE"
                     /locus_tag="OO130_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HCR5363960.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLHEFPDYTWQVAIADLEQSEAIGDRFGVFRFPTTLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            32862..33719
                     /locus_tag="OO130_000428"
     CDS             32862..33719
                     /locus_tag="OO130_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HCR5363961.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSKSEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            33853..35397
                     /gene="appC"
                     /locus_tag="OO130_000429"
     CDS             33853..35397
                     /gene="appC"
                     /locus_tag="OO130_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HCR5363962.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWAFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            35409..36545
                     /gene="appB"
                     /locus_tag="OO130_000430"
     CDS             35409..36545
                     /gene="appB"
                     /locus_tag="OO130_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCR5363963.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLSSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            36558..36650
                     /gene="cbdX"
                     /locus_tag="OO130_000431"
     CDS             36558..36650
                     /gene="cbdX"
                     /locus_tag="OO130_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HCR5363964.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            36730..38028
                     /gene="appA"
                     /locus_tag="OO130_000432"
     CDS             36730..38028
                     /gene="appA"
                     /locus_tag="OO130_000432"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HCR5363965.1"
                     /translation="MKAILIPFLSLLIPLTSQSAFAQSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYFGHYQRQRLVADGLLAKKGCPQP
                     GQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNV
                     NVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLKREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMAALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(38143..40323)
                     /gene="etk"
                     /locus_tag="OO130_000433"
     CDS             complement(38143..40323)
                     /gene="etk"
                     /locus_tag="OO130_000433"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HCR5363966.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLHDIVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGVEAPEQLEEHGISVYATIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSDAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(40343..40789)
                     /gene="etp"
                     /locus_tag="OO130_000434"
                     /gene_synonym="yccY"
     CDS             complement(40343..40789)
                     /gene="etp"
                     /locus_tag="OO130_000434"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HCR5363967.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            complement(40777..41916)
                     /locus_tag="OO130_000435"
     CDS             complement(40777..41916)
                     /locus_tag="OO130_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HCR5363968.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTRNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(41962..44058)
                     /locus_tag="OO130_000436"
     CDS             complement(41962..44058)
                     /locus_tag="OO130_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HCR5363969.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVNDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFEAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGQSEQLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            complement(44058..44804)
                     /locus_tag="OO130_000437"
     CDS             complement(44058..44804)
                     /locus_tag="OO130_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HCR5363970.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPGEQQTLSVGPMEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            complement(44801..45445)
                     /gene="gfcB"
                     /locus_tag="OO130_000438"
     CDS             complement(44801..45445)
                     /gene="gfcB"
                     /locus_tag="OO130_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HCR5363971.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGRNV
                     LCNVWQEEVTSTRPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            complement(45552..45857)
                     /locus_tag="OO130_000439"
     CDS             complement(45552..45857)
                     /locus_tag="OO130_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine-rich inner membrane protein GfcA"
                     /protein_id="HCR5363972.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEATNGTEATTGTTGTTTTTTG
                     ATTTATTTGGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            complement(46299..46511)
                     /gene="cspH"
                     /locus_tag="OO130_000440"
     CDS             complement(46299..46511)
                     /gene="cspH"
                     /locus_tag="OO130_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HCR5363973.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            46797..47009
                     /gene="cspG"
                     /locus_tag="OO130_000441"
     CDS             46797..47009
                     /gene="cspG"
                     /locus_tag="OO130_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HCR5363974.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            47020..47208
                     /locus_tag="OO130_000442"
     CDS             47020..47208
                     /locus_tag="OO130_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCR5363975.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVTLQIRTDHAPLDFTK"
BASE COUNT        11628 a        11730 c        12410 g        11455 t
ORIGIN      
        1 tagcgatgtg cagtcgggta aagtgaatgc cagcgcgaca tttgtacttc attatgacta
       61 atacgtggaa cagattggcg ctcttgattt tcgccgtttt atcgctgctg gtggcgggtg
      121 aattgcgggc aggagtggtg gtcggcggaa cgcgatttat ctttccggca gacagagaat
      181 cgatatctat tttactgact aataccagtc aggaatcctg gctgattaac agtaaaatca
      241 accgcccaac gcgttgggcc ggggggaaag cgtcgacagt gccagcacca ttactggccg
      301 ctccgccact tattctcctg aagcccggta ctacaggcac gttgcgcttg ctgagaacgg
      361 aaagcgacat cttgcctgtg gatcgcgaaa cgctatttga gttaagcatt gccagcgtgc
      421 catccggcaa agttgaaaat cagagcgtaa aagtggcgat gcgctcggta tttaaactgt
      481 tctggcgacc cgaaggtttg ccgggcgacc cgctggaagc ttatcaacaa ttacgctgga
      541 cacggaattc gcagggtgta caactcacta acccaacgcc ttactacatt aacctgattc
      601 aggtgagcgt aaatggtaaa gcgttaagta atgcgggagt tgtgccgcct aaaagccagc
      661 gtcagacaag ctggtgtcag gcaatcgcac cgtgtcatgt cgcctggcgg gcgataaatg
      721 attacgacgg gttgagtgca aagaaggagc aaaatctgcc ctgaaacagg ttcggaaaac
      781 gtttgcgttt tttttgccgc aggtcaattc ccttttggtc cgaactcgca cataatacgc
      841 ccccggtttg cacaccggga atccaggaga gttcatgtac taccccttcg ttcgtaaagc
      901 ccttttccag ctcgatccag agcgcgctca tgagtttact tttcagcaat tacgccgtat
      961 tacaggaacg ccgtttgaag cactggtgcg gcagaaagtg cctgcgaaac ctgttaactg
     1021 catgggcctg acgtttaaaa atccgcttgg tctggcagcc ggtcttgata aagacgggga
     1081 gtgcattgac gcgttaggcg cgatgggatt tggatcgatc gagatcggta ccgtcacgcc
     1141 acgtccacag ccaggtaatg acaagccgcg tctctttcgt ctggtagatg ccgaaggttt
     1201 gatcaaccgt atgggcttta ataatcttgg cgttgataac ctcgtagaga acgtaaaaaa
     1261 ggcccattat gatggcgtcc tgggtattaa catcggcaaa aataaagata cgccagtgga
     1321 gcagggcaaa gatgactatc tgatttgtat ggaaaaaatc tatgcctatg cgggatatat
     1381 cgccatcaat atttcatcgc cgaatacccc aggattacgc acgctgcaat atggtgaagc
     1441 gctggatgat ctcttaaccg cgattaaaaa taagcaaaat gatttgcaag cgatgcacca
     1501 taaatatgtg ccgatcgcag tgaagatcgc gccggatctt tctgaagaag aattgatcca
     1561 ggttgccgat agtttagttc gccataatat tgatggcgtt attgcaacca ataccacact
     1621 cgatcgttct cttgttcagg gaatgaaaaa ttgcgatcaa accggtggct taagtggtcg
     1681 tccgcttcag ttaaaaagca ccgaaattat tcgccgcttg tcactggaat taaacggtcg
     1741 cttaccgatc atcggtgttg gcggcatcga ctcggttatc gctgcgcgtg aaaagattgc
     1801 tgcgggtgcc tcactggtgc aaatttattc tggttttatt tttaaaggtc cgccgctgat
     1861 taaagaaatc gttacccata tctaattatt tcttcgaatt cctctttata agataatcag
     1921 ggctttattt tcagccctgg ttgttttata ttcatcgctg ttgcttattt agacattttg
     1981 tacttttatt gttgaggtta ttaagcgaag cgacaatgga ttgtgttgct gcggttttat
     2041 agggtagggg agaggcagat gcgaattaaa ccagacgata actggcgttg gtattacgat
     2101 gaagagcacg atcgaatgat gctcgattta gccaatggta tgctatttcg ctcacgtttt
     2161 gcgcgcaaaa tgttgactcc agatgccttt tctcccgcag gcttttgcgt tgacgacgcc
     2221 gcgctctatt tctcttttga agaaaagtgc cgcgatttta atttatctaa agaacaaaaa
     2281 gccgaactgg tgctgaatgt actggtagca attcgctatc tcaaaccgca aatgccgaaa
     2341 agctggcatt ttgtttccca tggtgaaatg tgggtgccca tgccgggcga tgcggcctgc
     2401 gtttggttga gcgatactca cgagcaggtc aatttgctgg tcgttgaatc tggcgaaaat
     2461 gccgcactat gcctgctggc acaaccctgc gttgttattg cgggtcgcgc gatgcagttg
     2521 ggcgacgcca ttaaaatcat gaacgacagg ctgaaaccgc aggttaatgt tgacagcttc
     2581 agcctcgaac aggcagtcta acgcgccaac ttaagtgcag tcttcggtac acagctacag
     2641 caaagaatgg tgccatcatc gcccattgct gattttttca gcggcgagac ttcgccttct
     2701 aaaatctgaa cacggcaact tccacaaatg cccgcgcggc aagaataagg gatacgaatt
     2761 ccctgatttt ctaattgctc cagcaacacc tgttggttat ttccacgaaa tgcctgtccc
     2821 tgccagtcaa tatctacatt tgcgtccggt tgttgcgtga tgttggcggt atcatcagcg
     2881 gcagctgcgc cgtaaatttt tgccggagcc gttgccagaa tttccacctc atcgccaacg
     2941 cgaatcacgc cgctattacg ggcaattaaa ttctgaccaa aatcgacatc gccgttatcc
     3001 tgggcagtgc ggaaagattg caatgttttt aatggttcgc ctgccggatg tttttgccct
     3061 ttttctgggc tgacggtggt gaaaatacag cggctacaag gtttaaccac atcaaacacc
     3121 acatcaccaa tgcgaatcac tttccagcga tcttcttccc aggctgacgc gccggaaacc
     3181 accagattgg ggcggaattg ctccattttt acactggccg gacaacgttg ttggagatca
     3241 cgtaacgagg cttcgttagc aagaaggtaa ggatagccat cagcaaatga cagaggtaca
     3301 gtgttgtggc gtttcacgcg ccgggtcatt tgtggcccca cccagcgtaa ttgcacttcg
     3361 cgggagaaaa atccacttag ccatttgttg atcgcgtctg gcgcaattcg cgcggtaaaa
     3421 tgtgtgcccc aaacttcggt tggtgcgtct tgtgtggcga aatcagcaaa acgaacatat
     3481 gcgctactgc catctggtgc ggtgagatgc aagccatcat gcacaggtga aggagtaaac
     3541 cgtaccatct ggggaaactg gcgagcggta ataaacgtac cgtcaggttc cgtgatcata
     3601 aagatgcgat cgaaggccag accactgaca tcagccagag catgtgtaag accaatgccg
     3661 cgcatcgatt taacaggatg aataaaaagc cgggttaatg tcgccacggt gcggtcctca
     3721 aatgaaaata agccctcaac tttatgacat gacgcgctta ttagctataa tgcgcaacaa
     3781 ttttcttagc gcctgtccca ttaggctatt ttatttgtca gtttggccct gggttgtgct
     3841 cgaaatcctc acgtacttgt gtacgctccg ttttctccgc gctgtccatg tccagactga
     3901 ctgcaacaat tacgcctact gcggtaggct cttagagtaa aagtgacgat atgaattctc
     3961 tgtttgccag tacggcccgt gggctggaag agctgttaaa aactgaactg gaaaacctgg
     4021 gggccgttga atgccaggtg gttcagggtg gggtccattt caagggcgac acacggcttg
     4081 tttaccagag cctgatgtgg agccgcctgg cctcgcgtat tatgttgccg ctgggcgagt
     4141 gtaaggttta cagcgattta gacctctatc tcggtgttca ggcgatcaac tggacagaga
     4201 tgtttaatcc tggcgcgacc ttcgctgtcc acttcagtgg tttgaatgac accatccgca
     4261 acagtcagta cggcgcgatg aaagtgaaag acgcgatcgt cgatgctttc acgcggaaaa
     4321 atctgccgcg tccaaatgtt gatcgcgatg cgccggatat ccgcgttaac gtctggctgc
     4381 ataaagaaac cgccagtatc gctcttgatc tgagtggtga tggtttacat ctgcgtggct
     4441 atcgcgatcg tgctggtatt gcgccgatca aagaaaccct ggcagccgct attgtgatgc
     4501 gatccggctg gcagcctgga acaccgctgc tcgatccgat gtgtggttcc ggtacgttgc
     4561 tgattgaagc ggcgatgctg gcgaccgatc gcgcacccgg cttgcaccgt gggcgttggg
     4621 gctttagcgg ctgggcgcag catgatgaag ctatctggca ggaagtgaaa gcggaagcgc
     4681 aaactcgcgc ccgtaaaggc ctggctgagt atagctctca tttttacggt tcggacagcg
     4741 acgcacgggt gattcaacgt gcacgcacta acgcccgtct tgcggggatt ggtgaactga
     4801 tcacctttga ggtgaaagat gtcgcgcaac tgaccaatcc gctgccgaaa gggccgtacg
     4861 gcacagtgtt gagcaacccg ccatacgggg aacgtctgga cagcgaaccg gcgctgattg
     4921 cgctgcatag cctgctgggt cggatcatga aaaaccagtt tggtggctgg aatctctctt
     4981 tgtttagtgc ctcgccggat ctgctaagct gcttgcagct gcgtgcagac aaacagtaca
     5041 aggcgaaaaa cggcccgctg gactgcgtac agaaaaatta ccacgttgcc gaaagcactc
     5101 cagacagcaa accggcgatg gtagcggaag actacgccaa ccgcctgcgt aagaacctca
     5161 aaaaattcga gaagtgggct cgccaggaag gaattgaatg ttaccgcctg tatgacgccg
     5221 atctgccgga atataacgtt gccgttgacc gttattccga ctgggtggtg gtgcaggagt
     5281 atgcaccgcc aaaaactatt gatgctcaca aagcgcgtca gcgtctgttc gatattatcg
     5341 ctgcaaccat ttcggtactg gggattgcgc caaacaaact ggtgctgaaa acccgtgaac
     5401 gccagaaggg caaaaaccag taccagaaac tgggcgagaa gggcgagttt cttgaagtta
     5461 ccgaatataa cgctcacttg tgggtgaacc tgacggatta tctcgatact ggtctgttcc
     5521 tcgatcaccg tatcgcccgc cgtatgctcg gtcagatgag caaaggcaaa gatttcctca
     5581 acctgttctc ttataccggt agcgccaccg tgcatgcggg attaggcggc gcgctcagca
     5641 ccaccaccgt ggatatgtcg cgtacttatc tggagtgggc agaacgcaac ctgcgtctga
     5701 atggcctaac cgggcgtgcg catcgcctga ttcaggccga ttgcctggcg tggctgcgtg
     5761 aggcaaatga acagtttgat ctgatcttta tcgatccgcc aaccttctct aactcaaaac
     5821 gaatggaaga tgcgtttgat gttcagcgcg atcatctggc gctgatgaaa gatttgaaac
     5881 gtctgctgcg tgcaggtggg acgatcatgt tctcgaacaa caaacgtggc ttccgtatgg
     5941 atctcgacgg cctggcaaaa ctgggactga aagcacaaga aattacgcaa aaaacgctct
     6001 cccaggattt cgcccgtaac cgccagatcc acaactgctg gctgattacc gcagcctgaa
     6061 aggaatagta atgtcattaa tcagtatgca tggcgcatgg ctgtcgttca gcgacgcgcc
     6121 gcttctcgat aacgcagaac tgcatatcga agataacgaa cgtgtttgtc tggtgggccg
     6181 caacggcgcg ggcaaatcga cgttaatgaa aatcctcaac cgtgaacaag ggctggatga
     6241 cggtcgcatt atttacgagc aagatttgat tgtagctcgt ctgcaacagg atccgccgcg
     6301 taacgttgag ggtagcgttt atgatttcgt tgccgaaggc attgaagaac aagcggaata
     6361 tctgaaacgc tatcacgata tttcgcgcct ggtgatgaac gacccgagcg agaaaaatct
     6421 caacgaactg gcgaaggttc aggaacagtt ggatcaccac aacctgtggc agctggaaaa
     6481 ccgcatcaac gaagtgctgg cgcaactggg gttagatcct aatgttgcgc tgtcgtcgct
     6541 ttccggcggc tggttgcgta aagcggcatt aggacgcgcg ctggtgagta atccgcgcgt
     6601 gctgttgctt gatgaaccga caaaccacct ggatattgaa accatcgact ggctggaagg
     6661 gttccttaaa accttcaatg ggacgattat tttcatctcc cacgaccgtt cgtttatccg
     6721 caatatggcg acgcgcattg ttgatctcga tcgcggcaag ctggtgacct atccagggaa
     6781 ttacgaccag tacctgcttg aaaaagaaga agccctgcgc gtggaagaac tacaaaatgc
     6841 cgagttcgat cgcaaactgg cgcaggaaga ggtgtggatc cgccagggga tcaaagcacg
     6901 ccgtacccgt aatgaaggcc gcgtacgcgc cctgaaagca atgcgtcgcg agcgtggtga
     6961 acgtcgcgaa gtgatgggta ccgcaaagat gcaggtggaa gaggccagcc gctccggtaa
     7021 gatcgttttc gaaatggaag acgtttgcta ccaggttgac ggtaagcaac tggtgaaaga
     7081 tttttcggcc caggttctac gtggcgacaa aattgtcctg attggtccga atgggtgcgg
     7141 caaaaccacg ctgctgaaac tgatgctcgg tcagcttcaa gcggacagcg ggcgtattca
     7201 cgttggcacc aaactggaag tggcttattt cgatcagcac cgcgcggaac tggatcccga
     7261 taaaacggtg atggataaca ttgccgaagg taagcaagag gtgatggtta acggcaagcc
     7321 acgccacgta ttgagctatt tgcaggactt tctgttccat ccgaaacggg cgatgacgcc
     7381 ggtacgtgcg ctttctggcg gtgagcggaa ccgcttgctg ctggcgcgtt tgttcctcaa
     7441 accaagcaac ttattgattc ttgacgaacc gaccaacgat cttgatgtcg aaacgctgga
     7501 actgctggaa gaactgatcg acagctatca gggcacggta ttgctggtta gccacgatcg
     7561 tcagtttgtc gataacaccg ttacagaatg ttggatcttc gaaggcggcg gtaaaattgg
     7621 tcgttatgtc ggcggttatc atgatgcccg tggtcagcaa gagcagtatg tggcgctcaa
     7681 acagcctgcg gtgaaaaaaa ccgaagaagc cgccgcggca aaagcagaaa ctgtaaaacg
     7741 cagcagtagc aaactaagct ataaattgca gcgcgaactg gagcagctac cgcaattgct
     7801 cgaagatctg gaggcgaagc tggaagccct acagacgcag gtggcggatg cttccttctt
     7861 cagtcagcca catgagcaga cacaaaaagt gctcgccgat atggctgctg cagagcagga
     7921 gctggagcaa gcctttgaac gctgggagta tcttgaagcg ttaaaaaatg gtggctgatc
     7981 gcaaaatagt cgatttaaac agagacagct gacaacggta aaattgcctg atgaactggg
     8041 tttatcaggc atgaaaacca acactttgtt agtcatctgt aaaatagcgc atcattaagg
     8101 agtaccaatg tgcgaacatc atcatgccgc gaagcacatc ctgtgctcgc agtgtgacat
     8161 gctggtggcg ttaccgcgcc ttgagcatgg tcagaaagcg gcatgtcccc ggtgtggcac
     8221 aacgttaacc gtggcgtggg atgcccctcg gcagcgtccg accgcctatg cgttggctgc
     8281 actgttcatg ctgttgctgt ccaacttgtt tccttttgtg aatatgaacg ttgcaggagt
     8341 taccagtgaa attacattac tggaaattcc cggcgtgctt ttttctgagg actacgccag
     8401 cctcggcacc tttttcctgt tgtttgtgca actggttccc gcgttttgtc tgataaccat
     8461 tctgttactg gtgaatcgcg cggaattacc ggtccgttta aaagagcaac tggcacgggt
     8521 gctttttcaa ctcaaaacct ggggaatggc ggagattttc ctcgcgggtg tgctggtcag
     8581 tttcgttaaa ctgatggctt acggcagcat tggggtaggc agcagctttc tcccctggtg
     8641 tttattttgt gtcctgcaac tgcgcgcttt tcagtgcgtt gatcgtcgct ggttatggga
     8701 cgacatcgcc ccgatgccag aactgcgcca gccgctaaaa ccaggcgtca cggggatacg
     8761 tcaggggctg cgttcgtgct cctgttgtac ggcaatcctt cctgctgatg aacccgtgtg
     8821 cccgcgttgt agtaccaaag ggtacgttcg gcgtagaaac agcctgcagt ggacactcgc
     8881 gctgcttgta acgtccatca tgctgtatct tccggctaat attttgccca tcatggtgac
     8941 ggatttatta ggctcgaaga tgccgtcgac gattctcgct ggggtcattc tgttatggag
     9001 cgaaggctct tatcccgtcg ctgcggtgat ctttctggcc agtattatgg tgccaacgtt
     9061 aaagatgatc gccatcgcgt ggctgtgttg ggatgccaaa gggcatggca agcgcgacag
     9121 tgaaagaatg catttgattt atgaagttgt tgagtttgta ggccgctggt cgatgattga
     9181 cgttttcgtt atcgcggtgc tctcggcgct ggtgcgtatg ggaggtttaa tgagtattta
     9241 tccggcaatg ggtgcattaa tgtttgcttt agtcgtcata atgacaatgt tttctgctat
     9301 gacgtttgac ccgcgtttgt cgtgggatcg tcaacctgaa tcagagcatg aggagtcctg
     9361 acagtatgga atctaataat ggggaagcca aaatccagaa agtgaagaac tggtctcccg
     9421 tgtggatatt tcctatcgtc acggcgctca ttggggcctg ggttctattt tatcattaca
     9481 gccatcaggg accggaagtg accctgatca ccgcgaatgc ggaaggaatt gaaggtggca
     9541 aaaccaccat taaaagccgt agcgttgacg tcggcgtggt tgaaagcgcc acactggctg
     9601 atgatttgac gcacgttgaa atcaaagcgc ggctgaattc cggtatggaa aaattgctgc
     9661 ataaagacac cgtcttttgg gtggtgaaac cgcagattgg tcgcgaaggg attagcggcc
     9721 tgggaacgct gctgtctgga gtttatatcg aactgcagcc aggcgcgaaa ggcagcaaaa
     9781 tggataaata cgatttgctg gactcgccac cgttggcccc gcctgatgcg aaaggtatcc
     9841 gtgtgattct cgatagcaaa aaagccgggc agctctcgcc aggagatccg gtgctgttcc
     9901 gtggctatcg ggtaggttcg gttgaaacca gcaccttcga tacgcaaaaa cgcaatatca
     9961 gttatcaact gttcatcaat gcaccttatg accgactggt gaccagcaat gttcgcttct
    10021 ggaaagatag tggcattgcg gttgatctga cgtcagcggg gatgcgtgtg gagatgggct
    10081 cattgacaac gctgcttagt ggcggtgtta gctttgatgt gccggaaggt ctggatttag
    10141 ggcagccagt ggcaccgaaa acagctttcg ttttgtatga tgatcagaag agcattcagg
    10201 attcgttgta caccgatcac attgattatc tgatgttctt taaagattcg gtacgcggtc
    10261 tgcaaccggg agctccggta gaattccggg gtattcgcct gggtaccgta agcaaagtgc
    10321 cattctttgc gccgaatatg cgtcagacat ttaacgatga ttaccgtatt ccggtactga
    10381 ttcgtatcga gccagagcgg ctgaaaatgc agcttggcga aaatacggat gttgttgagc
    10441 accttggcga attgttgaaa cgtggtttac gcggatcgct gaaaaccgga aacctggtca
    10501 ctggtgcact gtatgttgat ctcgatttct atccaaatac gcctgcaata accggtattc
    10561 gtgaatttaa tggttatcag attatcccga ctgttagcgg cggcctggcg caaatccagc
    10621 aacgactgat ggaagcgttg gataagatca acaaactgcc attgaatccg atgattgaac
    10681 aggcaaccag tacgctttct gaaagtcagc gcacaatgaa aaacctgcaa acgacgctgg
    10741 atagcatgaa caagatcctc gctagccagt cgatgcagca gttgccgacg gatatgcagt
    10801 caacgttgcg tgaattgaat cgcagcatgc agggcttcca gcctggctcc gcagcctaca
    10861 acaagatggt ggcggatatg cagcgccttg atcaggtgtt gcgagaactg caaccggtgc
    10921 tgaaaacgct caatgagaag agtaacgcgc tggtatttga agcgaaggac aaaaaagatc
    10981 cagagccgaa gagggcgaaa caatgaaaaa gtggctagtg acgattgcag cactgtggct
    11041 ggccggatgc agctccggcg aaattaataa aaactattac cagttacctg tggtgcagag
    11101 cggtacacaa agtaccgcca gccagggcaa tcgtctgtta tgggtagagc aggtcactgt
    11161 tcctgactat ctggcgggga atggtgtggt ttatcaaacc agtgatgtga agtatgtgat
    11221 tgccaacaac aacttgtggg ccagcccgtt ggatcaacag ttgcgcaaca ccctggttgc
    11281 caacctgagt acgcaactgc ccggctgggt ggttgcctcc cagcctctgg gaagcgtcca
    11341 ggacacgctc aatgttaccg taacggagtt taacggtcgc tatgatggca aggtcattgt
    11401 cagtggtgag tggctgttga accaccaggg acaactgatc aaacgtccgt tccgtctgga
    11461 aggagtgcaa actcaggatg gttacgatga gatggttaaa gtgctggccg gtgtctggag
    11521 tcaggaagcc gcttctattg cacaagagat aaagcgtcta ccttaattat aaagatttgt
    11581 aagtataacc gtctccggta tgttgcctga ggcggttttt ttgtttctaa cgtgcggaaa
    11641 aatttgttcc tcttcacatt ttttgtacaa ccgacatgcc cgtgtagctc acaaatatga
    11701 cagtggcgtg aattttgcgc attgacggca gttatgattc gcggtattgc ttaactgtga
    11761 ttgcacattt agtaatcact gttttctttt ccaccagaaa ccagtatgag ggaaacgagg
    11821 catgaagaga caaaaacgag atcgcctgga acgggcacat caacgtggtt atcaggccgg
    11881 catcgccgga cgctcaaaag aaatgtgtcc ctatcagacg ctgaatcaaa ggtcacaatg
    11941 gctgggaggc tggcgagaag ccatggcgga cagggtagta atggcctgat tctgtctctt
    12001 taaaaagaaa cctccgcatt gcggaggttt cgccttttga tactctgtct gatgataatc
    12061 agaaggcaga cgtatcctgg aacagaccga ctttcaggtc gctggcggta tagatcagac
    12121 gaccatcaac cagcacttcg ccatctgcca gacccataat cagacgacga ttaacaatgc
    12181 gtttaaagtg aatacggtag gtcacttttt tcgctgtcgg cagtacctga ccagtgaatt
    12241 tcacttcgcc aacgcccagc gcgcggcctt taccttcgcc gcccagccag ccgaggtaga
    12301 accctactag ctgccacatt gcgtccaggc ccaggcatcc cggcataacc ggatcgccaa
    12361 tgaagtggca tccgaagaac cacagatccg gattgatatc cagttctgct tcaacatacc
    12421 ctttgtcgaa gttaccaccc gtttcggtca ttttgaccac acggtccatc atcagcatgt
    12481 tcggtgctgg caattgcggg cctttagcgc caaacagttc accgcgacca gaggcaagaa
    12541 ggtcttcttt tgtataggat tcgcgtttat ctaccatgtt ctctgtaagc cttattttat
    12601 tgaagcacgc aggatagcta acacgtgtac gctgaacaag tccgatcagt tcggaataaa
    12661 ccagttcagc caacgtaatg gccatggaaa acggtgacgt ccttcctgtt gcgatgcttg
    12721 cgcgatacgt tcctggatgg tttgcatcag cgtcgtttgg ccttcgccat cccacaccag
    12781 atttaataat aacggcagtg cgtcagtcac atcgtctact gcccagatgg tgaatttgcc
    12841 ttcttctacc gctttcacca gttcactgtg aagacttaaa tggcgaacgt tagctgtggg
    12901 gataatgaca ccttgtttcc cggttaactc acgttgctgg caaatagcaa agaagccttc
    12961 gattttctca tttaaaccac cgaccggctg ggcgcgaccg aactgatcga ctgaacctgt
    13021 gatagcgata ctctgattca ccggcacatc ggcgagggcg cttatcaggg cgcagagttc
    13081 agccatcgag gcactatctc catcaacttc actgtatgac tgctcaaatg tcagcgatgc
    13141 tgagaagggg atctgttgct caagctgtag ttccgacatc aggaacgctt gcatgatcat
    13201 catccctttc gcatggatat tgccgccaag ttccgctttg cgttcgatgt cggtgaattc
    13261 accatcgcca atatgcacaa cgcagctaat gcgagaaggt tcgccaaaag cgcgtggatg
    13321 acccggaaat tcaatgaccg aaagggcgtt aatttgcccg atgcgttcgc cttcggtttc
    13381 aatcaggatt tgctcctgaa ggatctcatc ctgcatacgt tcagcgagga aaccttcgcg
    13441 ccattcacgc tgctgcagca ttaagtttag ctgctcgccg gagaaggtgt cgccatcaca
    13501 cagggaggcg acctctttac actggcggag gatccactgc gggctaagcg gaagtgtttc
    13561 ttgttcaccg gtgtagcgtg ctgcttcgcg gataagtacc ggccaggcat ccgctcccgg
    13621 ggcaggtaag tgattatgtc tggcggtaaa tgtcacccag cgacaccact ggcttactga
    13681 ctccgcatcg acaatctgca gagtatcttc aaattcgcta taaatagcct gctctgaaag
    13741 ctctggctcc atctcctgga aatcagccaa tgattcgcgt tcgcctacca gaatgacttt
    13801 cagcttcaat ggcatcgaag gcacagagac agggagaggg cgcgactcat caaacgcaac
    13861 ccagtcaaaa cgctcgcggt taacgatatt tttcagccgc atccacagca gaggttgcgc
    13921 cagcagtgta cgcaaagaga tgatgagaat accgccattt gcctgatgca ccagaccagg
    13981 ctgcagggta atgtcgccat taaactggcg caggcagcca aagagttgct ccgcttctac
    14041 ccagtcggca gcgacaactt gcgttaaagt cgcaaaatta tcatctgcac tcactgcgtg
    14101 acgtaagcgg atggagtggc cggaaacctc atagtgatcg cccaccagtt ggcctgcatc
    14161 gctttgtagc gtacgcgcgg catcggcaat aagattcaga tactcagact cttccggggc
    14221 cttagccagc ataaaagagg aggatgctcg cgtatgcagc aactgctcca gcgcaaattg
    14281 cagccgcggt tgagtatcac tgaataaagg atcgttttcg tcaatcaaat ttggctgagc
    14341 aaatatttcc tgatagctat cggtatcagg aaccaggtca cgccatgcaa gtttcgtaat
    14401 ggtcaaagtt gatgtttttt agtctgttgt caaagccgcg attataccgt aaccggcact
    14461 acagcacacg tagaaagcac cgacaatact cctggcatgg gcgttaaagc tcacaggatg
    14521 gagattcttt tcttcactgg cctaaaaagc tgatattctg taaagagtta cacggtaaca
    14581 ttgagatcgc tatgaaatat caacaacttg aaaatcttga aagcggttgg aaatggaagt
    14641 atctggtgaa gaagcatcgc gaaggggagt taattacccg ttacatagaa gccagtgccg
    14701 cccaggaagc cgtcgatgtg ttgctctcgc tggaaaatga acccgtgctg gtaaatggct
    14761 ggattgacaa gcatatgaat ccggaactgg tcaatcgcat gaagcaaacc attcgggcca
    14821 gacggaagcg ccattttaat gcggaacacc agcatacgcg caaaaagtcg atcgatctgg
    14881 aatttattgt ctggcaacgt ctggctggtc ttgcgcagcg tcgcggtaaa acgctttctg
    14941 aaacgattgt tcagctgatt gaagatgcgg aaaacaaaga gaaatacgcg aataaaatgt
    15001 cttctctgaa gctggatctg caggcattgc tgggtaagga ataactgacg aaagtcagtt
    15061 caatttacta aaggcaaaaa aaaccccgca gcagcggggt ttttctacca gacgataact
    15121 taagcctgcg gctgagttac aacgtctttg atacctttaa cttcgatctc tacgcgacga
    15181 tccggagcca ggcagtcgat cagtgcagca cgctgtttca cgttgtcaca ggtgttgcca
    15241 gtaaccgggt tggattcgcc cataccacgt gcggagatct tgtctgccgg gatacctttg
    15301 gagatcaggt aatcaacaac agactgagca cggcgctcgg acagaccctg gttgtaagcg
    15361 tcagaaccga tgcggtcggt gtaacccaga acaactacgg aaccgtcttt cggatccagg
    15421 ttgctcagct ggctgtacag ctgatccaga gcagcctgac cttccggttt cagggttgct
    15481 ttgttgaagt tgaacagaac gtcagacttc agagtgaagt gcttggtctg tacttccggt
    15541 gccggagctg gagccggagc aactactgga gctgcttcgc cctgaccgaa acggtaggaa
    15601 acacccaagc tcagcatgcc gttgtccgga cgagtgccga tggtgtgtgc gtcaccgatg
    15661 ttgttggtcc actggtattc cagacgggta gcgatttcag gagtgatcgc gtactcaaca
    15721 ccgccagcga agaccggaga aacgccggtg tcgtggtttt taccataaac gttggattta
    15781 gtgtctgcac gccataccat accacccaga cgagtgtaga tgtccaggtc gtcagtgatt
    15841 gggtaaccca gtttagcggt cagttgaacg ccctgagctt tgtatgcacc gttttcaacg
    15901 ctgcctttgt acggcatacg acctaaccag tcgtaaccca tttcaaagcc aacatacggg
    15961 ttaacctggt aaccaccaaa agcaccagcg cccagttggt tttcatgggt cgggccattg
    16021 ttgttgatga aaccagtgtc atggtactgg gaccagccca gtttagcacc agtgtaccag
    16081 gtgttatctt tcggagcggc ctgcgctacg gtagcgaaac cagccagtgc cactgcaatc
    16141 gcgatagctg tctttttcat tttttgcgcc tcgttatcat ccaaaatacg ccatgaatat
    16201 ctccaacgag ataacacggt taaatccttc accgggggat ctgctcaata ttaactctac
    16261 cgatatcttc ggcttatgcc gagcacccct ggcgatgtaa agtctacaac gtagttgaaa
    16321 acttacaagt gtgaactccg tcaggcatat gaaaaaaaag tcttgtataa ggtatgttta
    16381 atcttttttg tcagcgacaa tttacagaag aaaatcgcgg aaaccgcttc agacaagcct
    16441 ccgcaaggaa aattagtcac gactgaaagc attggctggg cgacaaaaaa agttccagga
    16501 ttaatcctaa atttacttaa tgatacaaat tagagtgaat ttttagcccg gaaagttgtc
    16561 tcgtggcgtg agaggatgcg cttaccggac gcataataaa ccccatagcg ttaccttcat
    16621 ttgccgcatc aacaagttca gcatgctctt cttcagtcaa atcatctgcc aaccaaccga
    16681 tcaccacact gtaattgccc gtgcgtaaag cgcgaaccat tgactccaca gtgtggcaag
    16741 gggagagctg gctaatctgc attactttcg ttaagggtag cccagatgcc tgaacccatt
    16801 cccgactcag tttttgttgc ggtgttaacc agagttgcca gcgcgattgc tgaccgagtt
    16861 gctgtaacaa tggcaacagt agaagttgcg tcatcatggg ctgatcttcg cgatagacaa
    16921 cttcactgat aagcccggct gtagtgtttt ccgtagagac acgcgcaatt ttacttgctg
    16981 cggatgagaa cgacgaagaa cgatgtgcat agcctgaagt gtacataatc aatccagccc
    17041 ctgtgagtta ctgtatgaat atacagtaca tccagtgaca acaaagatca accctatttt
    17101 cggaaagagc ctcgcaaatt ttttcgttgg tgacgggaaa acataaatta atcttgcccc
    17161 ttaagaataa gttgcctatt ttcgtagtta acggatccgt taatgtgaat cattctttta
    17221 tgttatgatt ttaaaaggaa ttttatgaaa agcctctcct ataagcggat ctataaatca
    17281 caagaatacc tggcaatgtt gggcacaatt gaataccgat cattgtttgg cagttacagc
    17341 ctgaccgttg acgacacggt gtttgcgatg gtttctgatg gtgagttgta tcttcgggct
    17401 tgtgagcaaa gtgcacagta ctgtgtaaaa catccgcctg tctggctgac atataaaaag
    17461 tgtggccgat ccgttaccct taattactat cgggttgatg aaagtctatg gcgaaatcaa
    17521 ctgaagctgg tgcgtctgtc gaaatattct ctcgatgcag cgctgaaaga gaaaagcacg
    17581 cgcaataccc gggaaagact gaaagatttg cccaatatgt cttttcatct ggaagcgatt
    17641 ctcggggagg tggggattaa ggatgtacgg gcgttacgta tacttggggc aaaaatgtgt
    17701 tggttgcgac tgcggcagca aaacagtctg gtgacagaaa agattctgtt tatgcttgaa
    17761 ggtgccatta tcggcattca tgaagctgcg ctcccggtgg cacgccgcca ggagcttgca
    17821 gaatgggctg actcacttac gccgaaacag gagtttcctg cggaacttga gtaatctggc
    17881 gttgcagacg accaatctca ggcagtaatg caatcaataa tccaacttgt tgtacgacca
    17941 gaggttcttt gctgtctgcc cgtggttcaa gttgctgcat ccgctgtttc aggctagcta
    18001 atgcctcatt gacgcgttct tcatcagcag gttgatgatg taacgcgtca tcaacatagc
    18061 aaactgcgtc atcaagaaac gccagaattt caggattagt taactgctcc cggtgagcac
    18121 cgagggctga gatatagctg gtaaacgtat ggttaaggca cagcaaccga aacgcggctt
    18181 cgcgaatttg cggggtaacg ttcggctcgc tggacatatt tgataccacc gacgccagct
    18241 cagcatcacg gttgtgtgca tcgcggcggg caatacgata cgccagacgg ttatcacgcc
    18301 cctgatggta ttgctccagt atggcatcga gataccgaca gttggcctct gtggcgcgtt
    18361 cgagcatgcg cggcaaattg cgaaactgcc agtcaggcca gatgtagctc actgccgccc
    18421 acgcaatggc acaaccaatc agcgtatcga ttacgcgagg taacgctact tcaaaacctt
    18481 cacccagtaa gttaaaacac agtagcacca aaagtgtgat gaacatcgtt gcatgagcgt
    18541 attgcacgtt acggaaggca aaaaagagca cgccggtaat aaccagcagc accagctgcc
    18601 cttccagtga tggcacaaac cacagcacag gaatgccaat ggcgatacct accagcgtac
    18661 caataatcct taacttcagg cggtggcgcg tggcgttata gtttggctgg cagacaaaca
    18721 aacttgtcag caagatccaa aacccgtgat gcattccggt tatttgaatg atggcgtagc
    18781 cgaagcacaa caccagcgac attcttaccg catgacggaa gagggcggat tccggcgtga
    18841 agtgacggct aagacgcagc cagatatcac tcaacccgtg cgggctgtca tcagcgagct
    18901 cattttcgtc attattatgg ggtagtgcct gggcctgttc tgattcaatt gttgccagtt
    18961 gggcatcaat ggcgcgtaaa ttgttcagca aaaatcccag tgttttgagt aaatcggcgg
    19021 gtgcgccgtt atcgcgcatc cgctccagcg cagcatcaat atgcgtaaaa gcgcgctcaa
    19081 aatgcggatc atgttgataa ggctgacgca acaaaataca gcgtgacagt tgctggcacg
    19141 cctggccctg catcgacatc agccgctgaa aacggaacag cacgtcgctg tggcgaaaat
    19201 gttcacgcaa tgtttgatac tgaatatgag aagagctggc acgctcgtga atatcctgtg
    19261 cgacaaagta ataatgcagc gtgcgacgcg ttccccgttg accacgatcg ccacgtaagc
    19321 gggtcagcag cgagagtttc gtctgattca atgtcgccat cagcagaccg ttggcgagag
    19381 ccaaatcgta cagcggtgcc tggctttcat cttcaatatc aggatcaaac atgcgcgact
    19441 tgagctcaag ataacgcgcc agttgttcat agcaacgcgc caggttgtcc tgcagcgggc
    19501 ggaccgggaa cagcagatga ccaataagtg tcaggacgtt gtaccagacg gcaccggcca
    19561 gcagatacat cggctgctga taccagtgct catacagtga tgttcccaac atagtgtaaa
    19621 tggcgatcag caatgcaccg aaggcaattg ttgcatagcg ttgacccaga ccgccgagca
    19681 aaatgaagcc gctggtagag agcgttaagc caatcgcaaa tagccagggc cagggaaaca
    19741 gcaattctac tgaggccgag gcgataaaaa agcagaacag cgtaatgatg aggttacgca
    19801 aacgtcccgc cagtcggtca tcgagatcgg tcagcgccgc tgccaccatc cccagcgtta
    19861 gcggaatcgt cagttttaca tcacccagcc accacggaaa cgctgtggtt ccacaaagcg
    19921 caataaaaat acgcgcgtaa tacagccagg cgctgttcca ggtatagcgt ttgagcaaag
    19981 gacttagcat aaaggccata ttagtggatt attcaaaacg acgacgtgca ttggcttcac
    20041 gcgcagcttg cgctgtttct accgatacca cgcgacgacc aaccggccat agtgcaatag
    20101 cggcaatttt aaagttcgca atgccgacag gaatgccaat gattgaaata cattgtgcga
    20161 tgcccgttgc aatgtgcatc aggcataacc accagccaaa gaaaatcagc cagaaaatat
    20221 tcaataccgt accgccagta ttcagcagca catttttgcc agccgggttc agttcatcga
    20281 catgaatagc ttcattgcca taaggcacca gagacagttt agtgatctcc cagcaggatc
    20341 gtgtcagcgg taaggtaaaa atcagcacaa tactgaccag agtcgccaac agccagccca
    20401 gagtggtggc aaatccgcca agcacaaagt tcagaatgtt caaaacggta cgcataaaac
    20461 ctcgctttac tgtggttttc agtaatgggc ggcaattgta acgttttttt gggctggagc
    20521 acgttttctc tgacggttac actgataaga aataatttcg tgtggatcta cagagtcatg
    20581 gaactgaaag cgacaacgct tggaaaacgt ctggcacagc acccttacga tcgggcggtg
    20641 atcctcaatg ccgggattaa agtctccggc gatcgccacg aataccttat tcctttcaat
    20701 caattactgg cgattcactg taagcgtggt ctggtatggg gcgagctgga atttgtactg
    20761 ccggacgaaa aagtggtgcg tctgcacggc accgaatggg gcgagacgca gcgtttttac
    20821 catcatcttg atgctcactg gcggcggtgg agtggcgaga tgagcgaaat tgcgtctggt
    20881 gttttacgcc agcaactgga tttgattgcc acgcgcactg gagaaaataa atggctgacg
    20941 cgtgagcaaa cctctggcgt tcagcaacaa atccgccagg ctttgtcggc gttgccgttg
    21001 ccgttgccgg ttaaccgact ggaagaattc gataactgcc gtgaggcgtg gcgtaaatgt
    21061 caggcctggt tgaaagatat tgaaagcgct cggttgcagc ataaccaggc gtataccgaa
    21121 gccatgctta ccgagtatgc ggattttttc cgccaggtcg agtcttcacc gctgaatccg
    21181 gcgcaggccc gggcagtcgt taatggcgag cattctctgt tagtgctggc aggtgcagga
    21241 agcggaaaaa cgtcggtgct ggtggcccgt gcaggctggt tgctggcacg tggtgaagcg
    21301 tcccctgagc aaattttatt gctggcgttt ggtcgcaaag ccgctgaaga gatggacgag
    21361 cggattcgcg aacggctaca taccgaagac attaccgcac gcacgtttca tgcgctggcg
    21421 ctgcatatta ttcagcaggg cagcaaaaaa gttccgatag tcagcaaact ggaaaatgat
    21481 accgctgccc gtcatgaact ctttattgct gagtggcgca agcaatgcag cgaaaagaaa
    21541 gcgcaggcga agggctggcg gcaatggctg acggaagaaa tgcagtggtc agtgccagaa
    21601 ggtaacttct gggatgatga aaaattacag cgtcgccttg cctctcgcct cgatcgctgg
    21661 gtaagtctga tgcggatgca cggtggtgca caggcagaaa tgattgccag tgcacccgaa
    21721 gagattcgcg atctgttcag taaacgtatc aagttgatgg ccccgttatt aaaagcctgg
    21781 aaaggtgcgc tgaaggcaga aaacgctgtc gatttttcgg gccttattca tcaggcgatt
    21841 gtgattctgg agaaaggtcg ctttatcagc ccgtggaagc atattctggt tgatgaattt
    21901 caggatatct cgccgcagcg ggcagcgttg ttagcggcat tacgcaagca aaacagtcag
    21961 acgacgttgt tcgctgttgg tgatgactgg caggcgattt accgattcag cggtgcgcaa
    22021 atgtcgctca ccaccgcttt ccatgaaaac tttggtgaag gcgaacgttg tgatttagac
    22081 acgacttacc gttttaacag tcgtatcggt aaggtggcaa accgttttat tcagcagaac
    22141 ccaggccagc tgaaaaagcc gctaaacagc ttaaccaatg gagacaaaaa agccgtcacg
    22201 ttattggatg agagtcaact tgacgctttg ctggataagc tctctggtta tgccaaaccg
    22261 gaagagcgca ttctgatcct ggcgcgttac catcacatga ggcctgccag cctggaaaaa
    22321 gcggcaacac gctggccgaa gttgcaaatc gactttatga ccattcatgc cagcaaaggg
    22381 caacaggcgg attacgtcat catcgttggc ttgcaggagg gaagtgatgg ttttccggct
    22441 gcggcgcggg agtcgattat ggaagaggcg ctactgccac cggttgagga tttcccggac
    22501 gctgaagaac ggcggttaat gtacgtggcg ctgacccggg cacgccatcg ggtatgggca
    22561 ctgtttaaca aagagaatcc ctctcccttt gttgaaatac tgaaaaatct ggatgtgccg
    22621 gtggcgagaa aaccgtaaga aacaggtggc gtttgccacc tgtgcaatat tacttcagac
    22681 ggtccgcgag ataacgctga taatcgggga tcagaatatc gaccgcgtcg ttgaaatgcg
    22741 gcgactggat tatgaagtct gccgttgcca cgttggcggc gaccggaatg ttccataccg
    22801 tcgccagacg cagcaaggct ttcacgtcag gatcgtgcgg cacggcattt agtggatccc
    22861 agaagaaaat caatacatca attttccctt ctgagatcaa tgcgccaacc tgctggtcac
    22921 cccccattgg gccactcaac atcgcgttga cgttcatgcc ggtcgcgcgg gaaattaagt
    22981 taccggtagt gcctgttgca tacagtacgt gttgttccag taacggttga tgccgttcca
    23041 cccagctcat cagcatttgt ttgcagtgat cgtgtgccac cagcgcaata tgtttccgcg
    23101 caggtaaagt gcgagtcgtc agttccataa tgtacatccg tagtcaactt tcctacagat
    23161 tactgtaagc acttatcgct gcaagataaa gaccgaaaaa gcctgcgcac aggcacaaaa
    23221 atctcaggaa gatggttgtt tttccgccca ctgcaggaaa gtatttcgcg tttgtgggtc
    23281 agccagttta aaccaatact tcagccgttg ttctgtgagc acctgagact gcggtggaat
    23341 agcatccagc tcggaaacac ccgaaagcag cgtactgtca tctgccatca tcgtggcaaa
    23401 ttgcggtagt gaagcgcgtt tcgcggattt gttatagcgt tcaacctcga cctcataatc
    23461 aatcgttttt gccgttgagg taatggcgag aatatccagc tttaccggaa tcggcgtcgc
    23521 atcgccatcc aacaattcaa ggcgcggcgc ggcatcaaaa tggttagctt cccgctcatt
    23581 ctccaggcga ggcaggcgaa aattgacctg gttgatgagc tgggtattaa aactgaccac
    23641 tagcggtggg gagatataca gccgttcttc actattggaa agatgaatcg tcttctcaac
    23701 gcgaaacact aactgatgcg gtccattatc cagttcaatg ctgtccgcac cgcgcagcag
    23761 agagctggaa accttcttgc cgtcgagtac cagaagatcg acatcggttg aaagccgcag
    23821 cgtggtggca aaaacggata ccggcaaaca taatgcaatc aaggtggtca cgatgccggt
    23881 tttcataggt agctcctgtc aaaagaccgc ccgaataata atgtgtcaaa atttttcttt
    23941 aatcatactt actaacatat agacatattt cccgcttttg ctctcattca ttcgtattag
    24001 ctgcatggtt ggcatgtcgg ctttgtcgta cactttgtta aacagtctgg aggaaataat
    24061 gaaagaaacc gatattgcag gcattttaac gtccactcac accattgcgc tggtgggggc
    24121 aagcgacaaa cccgatcgtc ccagctatcg cgtgatgaaa tatctactcg accaggggta
    24181 tcatgtcatt ccggtttcgc caaaagttgc cggcaaaacg ctgctgggcc agcaggggta
    24241 tggcacgctg gcagacgttc cggaaaaggt cgatatggtc gatgttttcc gtaattcaga
    24301 agcggcgtgg ggcgtggcac aggaagctat tgccattggc gcgaaaaccc tctggatgca
    24361 actgggggtg attaatgagc aagcggcagt actggcacgg gatgccgggt tgaatgtggt
    24421 gatggatcgc tgcccggcta ttgagatccc tcgcctgggc ctggccaaat aaaaaatccc
    24481 cggaaggcaa aaaccttccg gggattcgtt cagggatcag ttacgcagac gcggggcctg
    24541 gagttgtttg cggatggtct gcgccagctc atccatggat ggctgttcag gatgttcatc
    24601 ctgcaattcg ctacttagct gggcttcagc caggtaggta tgaaccggta ggccgttatc
    24661 gtcctccatc actacgtgat accacggagc ggcgcgaagc tcgtcattta ccgccagctc
    24721 atcaggcgac ggttcagaga gcgaataaac cgggtcgata tccacgacca ctccgagata
    24781 acctaacagg gaatggcgga cctgctggcc gataccgaat ttgctggcaa tcatagtcac
    24841 ctcccgggaa atctttacct ttgatataag ggtaaaattc cacatttcaa gttacatgac
    24901 gcgacaggca aaccctttca gatatagccc ttccggatag gtagcgatca ccggatgatc
    24961 ggctgcctga cggaactgct ctataaattg tacatcacgg ccggcatcaa ttgcggcatc
    25021 cgcgatgatt ttctgaaata aatcgctggt catcagaccg gaacaggaga aagtcaggag
    25081 aataccgcct tcattcagca actgaatcgc cagcatgttg atatctttat agccacgaca
    25141 cgcgcccatc aactggcttt tattctcaac aaacttcggc gggtccatca cgataacgtc
    25201 aaatttttca ccgcgatcgc gataagtacg cagcaattta aagacatcat cacggacaaa
    25261 ctcagccttg ctcagatcca gtttgttcag ctcaacgttc tgccgtgcaa tatccagcgc
    25321 ttcctgggag gtatcaacgc tgacaacctg gctgcaaccg cccatcagtg ccgatacggc
    25381 gaaaccaccg gtataggaga aacagttcag cacacgttta ttttcaacgt agcggcgggt
    25441 agccaggcgg ctgtctcgct ggtccaggta gtagcccgtt ttgtgtccgt gctgaatatc
    25501 caccagcagc ttcatgccgt gttcttcaat cggcagcagg gcaggcggca actcgccggt
    25561 gacgaggccc agggtcagct ccatcccttc ttttttacgt accgcaacat cgctgcgatc
    25621 gtaaatcgca cattccgggt acagcgtttg cagggcacta actaatgccg cgcgctgata
    25681 ttctgcccca gcactcagca gttgcagcac cagaaaatta ccgaaacgat cgatagtaat
    25741 acccggcagg ccatcagatt ctccggcgat taaacgatag ctgtcgaggc catctttttg
    25801 cgccagccag tcacgccatt tttgtgcttg ttgcaaacgg cgggaaaaaa aagcaatgtc
    25861 gatagactca gacgggtcaa acgtccagac gcgcgcccgg atttgcgaag ctggcgaata
    25921 agcgccgcgt gctaaccatt ttccctgatg atcaacaata tcgatggttt caccgaggct
    25981 ggctttacct tccatgcggg caacggcccc ggaaaagacc cacggatggc gacgaagtaa
    26041 tgatttttcg cgccctttgg ctaacactaa acgtacactc ataatttact tttctgtcga
    26101 tgcaaagaaa tgggcgtcat tgtccggagt tttccatgga tttgcaacgc gtccattaag
    26161 gataaggaga gcagcaatgt cgaaagtctg cataattgcc tgggtttacg ggcgagttca
    26221 gggcgtagga tttcgctaca ccacacagta cgaagcgaaa agactgggtt taaccgggta
    26281 cgccaaaaat cttgacgatg gcagcgtgga agtggttgcc tgcggtgaag aagggcaggt
    26341 ggaaaaatta atgcagtggt taaagagtgg cggcccgcgt tctgcgcgtg tagaacgggt
    26401 gctcagcgag ccgcatcatc cctcggggga attaaccgat tttcgtatcc gttaaataca
    26461 ttttaccggt tttggcaggc cagcaatttt ggtggcttgc ttagccggac ctttcggaaa
    26521 cagtcggtat aaatagcggc tgttaccttt ttcttcgcca aatttattcg ccatcgcttt
    26581 taccagcata cgaatcgccg gagaagtatt gaattccaga tagaaatcac gcacaaaacg
    26641 caccacttcc cagtgttccg gtgacagcga aatcccttcg ttctctgcaa tcaccactgc
    26701 cagcggctca ctccactggc tgctttcttt gagatagcct tcggtatccg tttctatctc
    26761 tttaccttcg aagatcagca taattattac taccttaatc agactggcgg cagtgtaaca
    26821 aaaaacaaag ccccgcataa agcgaggcta tgaaagtgtt agcgggtgag attaatcgcg
    26881 gctggcgaag cccagaatgc tcagcaggct gacgaagatg ttgtacagcg aaacatacag
    26941 gctaaccgtg gcacgaatat agttcgtttc accgccatga acgatgttgc tggtttcaaa
    27001 caaaatagcg ccagaggaga tcaaaatgaa gaccgcgctg atcgccagat gcagagcagg
    27061 cagctgcagg aagatattag caaccatacc aatcagcacc accacaatac ccgccatcag
    27121 cataccgccg aggaacgaca tatctttgcg ggtggtcagc acatatgcag agcagcagaa
    27181 gaacactaac gccgttccgc ccagagccat agcgattacg tcacccattc cggcagacag
    27241 ataggtgttc agaataggtc cgaggatata acccagaaaa ccggtaaagg cgaatgcgga
    27301 gataatcccg gtcggcttat tcgccgtttt ataggtcagg aacatcaaac catacatacc
    27361 caccagcgtc agaatcagac ccggagatgg cagcatcagc accgtactgg cagttgcggt
    27421 aatcgccgaa aaggccagcg tcaggctcag cagaaaatag gtattacgca gcaccttatg
    27481 ggtgctaagc agtgatgtac ggtcatgtga agaactaaca atacgatcca ttgagtcact
    27541 ctctatgaca gatgtaatta attaagcagc ataatgataa tgcgtaaggg cacccagaag
    27601 ttttacccat ctttacgcat ttgatctgga acaggtttaa cagcggatta tcaggtcatt
    27661 aagcaaatat aacgccctga gaatttcgac aggcaaaaga aaaaggggtt agcatttagc
    27721 taacccctta tcttatttgg cggaagcgca gagattcgaa ctctggaacc ctttcgggtc
    27781 gccggttttc aagaccggtg ccttcaaccg ctcggccaca cttccggaat gacgcgcact
    27841 ataaacatcc cgatgcggcg tgtaaacccc taatttgttt gtttgcctga aaaacagcca
    27901 aaagtgcatt gatagcgtga aataacagca gattgatcat ttcatcacca tgaattcctt
    27961 ctcttttact cgtttagcaa ccggctaaac atccccaccg cccggcaaaa agaaaaatag
    28021 gtccattttt atcgctaaaa gataaatcca cacagtttgt attgttttgt gcaaaagttt
    28081 cactacgctt tattaacaat actttctggc gacgtgcgcc agtgcagaag gatgagcttt
    28141 cgttttcagc atctcacgtg aagcgatggt ttgccttgct acagggacgt cgcttgccga
    28201 ccataagcgc ccggtgtcct gccggtgtcg caaggagaag agacgtgcga tatgaataac
    28261 gaggaaacat tttaccaggc catgcggcgt cagggcgtta cccggcgcag ctttctcaaa
    28321 tattgtagtc tggctgccac gtcgctggga ttaggcgcgg gaatggcacc aaagattgcc
    28381 tgggcgctgg agaacaaacc ccgcattccg gtggtatgga tccacggtct ggaatgcacc
    28441 tgctgtaccg aatcttttat ccgctccgct cacccactgg cgaaggacgt catcctttcc
    28501 ctgatttccc tcgattatga cgatactttg atggctgccg ccggaaccca ggcggaagaa
    28561 gtcttcgaag acatcatcac gcaatacaat ggcaaatata tcctcgcagt agaaggtaat
    28621 ccgccgctgg gcgagcaggg gatgttctgt atcagcagcg gtcgaccgtt tattgagaaa
    28681 ctcaaacgtg ccgctgccgg agccagcgcg attatcgcct ggggaacctg cgcgtcctgg
    28741 ggctgcgtgc aggccgcgcg gcccaatccg acgcaggcaa cgcctatcga caaagtcatc
    28801 accgacaaac ccattatcaa agtacctggc tgcccgccga tcccggatgt gatgagcgcc
    28861 atcattactt acatggtgac ctttgatcgc ttgccagatg tcgacagaat gggccgtccg
    28921 ctgatgttct atggtcagcg aatccacgat aaatgctatc gccgcgccca cttcgacgcc
    28981 ggagagttcg tccagagttg ggatgatgac gctgcccgca aaggttactg cctgtacaaa
    29041 atgggctgca aagggcctac cacctataac gcctgttcct ccacacgctg gaatgatggc
    29101 gtttctttcc caatccagtc tggtcacggc tgcctgggct gtgcggaaaa tggtttctgg
    29161 gatcgcggtt cgttctacag ccgcgtggtc gatattccgc aaatgggtac tcattccacc
    29221 gccgataccg tcggtttaac cgcgcttggc gtggtggcag cggctgttgg tgtgcacgca
    29281 gtcgccagcg ccgttgacca gcgcagacgt cataaccagc aacctacaga aaccgaacat
    29341 cagccaggca atgaggataa acaggcatga gcactcagta cgaaactcag ggatacacca
    29401 tcaataatgc cggacgccgc ctggtggtcg acccgattac gcgcatcgaa ggccacatgc
    29461 gctgcgaagt gaatattaac gatcagaatg tgatcaccaa tgccgtctcc tgcggcacca
    29521 tgtttcgcgg gctggagatc atcctacaag ggcgcgaccc gcgcgatgcg tgggcgttcg
    29581 ttgaacgtat ctgcggcgtc tgtactggcg tacacgccct ggcttcggtt tacgccatcg
    29641 aagatgctat cggtattaaa gtgccggaca acgccaatat catccgcaac attatgctgg
    29701 caacgctctg gtgccacgat catctggtgc acttctatca gcttgccggg atggactgga
    29761 tcgatgtgtt agatgcgctg aaagccgacc cgcggaaaac ctccgaactg gcgcaaagtc
    29821 tctcctcttg gccgaaatca tcccctggct atttcttcga cgtacaaaac cgcctgaaaa
    29881 aatttgttga aggcgggcag ttggggatct tccgcaatgg ctactggggg cacccgcagt
    29941 acaaactgcc gccagaagct aacctgatgg gctttgccca ctatctcgaa gctctcgatt
    30001 tccagcgtga aattgtcaaa atccacgcgg tctttggcgg taaaaacccg catccaaact
    30061 ggattgtcgg cgggatgcct tgcgccatca acattgacga aagcggcgcg gtcggggcag
    30121 tcaatatgga acgcctgaac ctggtgcagt caattatcac ccgcacggcg gacttcatta
    30181 acaacgtgat gatccccgac gccttagcca tcggtcagtt caacaaaccg tggagcgaaa
    30241 tcggcactgg tctttctgat aaatgcgttc tcagctacgg cgcattcccg gatattgcca
    30301 acgactttgg cgagaaaagt ctgctgatgc ctggcggcgc ggtgattaac ggcgacttca
    30361 acaatgtgct gccagtggat ttggttgatc cgcagcaggt gcaggagttt gtcgaccacg
    30421 cctggtatcg atatcccaac gatcaggtcg ggcgtcatcc gttcgatggc atcaccgacc
    30481 cgtggtacaa ccccggcgat gtcaaaggca gcgataccaa cattcagcag ctgaatgaac
    30541 aggaacgcta ctcgtggatc aaagcgccac gctggcgcgg taacgcgatg gaagtggggc
    30601 cgctggcgcg cacgttaatc gcttatcaca aaggcgatgc tgcgaccgtt gagtcggtcg
    30661 atcgcatgat gtcggcgttg aacctgccgc tttccggtat ccagtcaacg ttaggccgca
    30721 ttttgtgccg cgcgcacgaa gcgcagtggg ccgcaggtaa gttgcagtat ttcttcgaca
    30781 agctgatgat caacctgaaa aacggcaatc tcgccactgc ttccacggaa aaatgggaac
    30841 ctgcaacctg gccgacagag tgccgtggtg tcggttttac cgaagcgccg cgcggggcgt
    30901 taggccactg ggccgccatt cgcgatggca agattgatct ctaccagtgc gtggtgccga
    30961 ccacctggaa cgccagcccg cgcgatccca aagggcagat tggcgcttat gaagcggcgc
    31021 tgatgaacac caaaatggcg atccccgagc aaccgctgga gatcctgcgt actctgcaca
    31081 gctttgaccc gtgcctcgcc tgttcaacac acgtgctggg cgacgacggt agcgagctga
    31141 tctccgtgca ggtgcgttaa cagcgaagga gaatcatcat gcaacagaaa agcgacaacg
    31201 ttgtcagcca ctatgtcttt gaagcgccag tccgcatctg gcactggttg acggtgttat
    31261 gcatggcggt gttgatggtc accggatact ttatcggcaa gccgctacct tccgtcagcg
    31321 gcgaggcgac gtatctgttc tatatgggct acatcaggtt aattcacttc agcgccggga
    31381 tggtttttac cgtggttttg ctgatgcgga tctactgggc ttttgttggc aatcgatact
    31441 cccgcgagct gtttatcgtg ccggtatggc gtaaaagctg gtggcagggc gtgtggtatg
    31501 aaatccgctg gtatctgttt ctggcaaaac gtccgagtgc cgatataggc cataatccca
    31561 tcgcccaggc ggcgatgttc ggctatttcc tgatgtcggt ctttatgatc atcactggtt
    31621 ttgcgctgta cagcgaacac agccagtacg ctatttttgc gccgttccgt tatgtggtgg
    31681 aatttttcta ctggacgggt ggcaactcaa tggacattca cagctggcat cggctgggga
    31741 tgtggctgat tggcgcgttt gtgatcggtc atgtctacat ggcgctgcgt gaagacatca
    31801 tgtccgacgg cacggtgatc tccaccatgg tcaacggcta ccgtagccac aaatttggca
    31861 aaataagtaa caaggagtgt tcatgagcga gcaacgcgtg gtggtcatgg ggctgggcaa
    31921 cctgctgtgg gccgatgaag gcttcggcgt gcgggtggcg gaacggctgt atgcccatta
    31981 ccactggccc gagtatgtgg agattgtcga tggcggtact cagggactga acttgctggg
    32041 gtatgtcgaa agcgccagcc atctgttgat tctcgatgcc attgactacg ggctggaacc
    32101 tggaacgctg cgaacctatg ccggagaacg cattccggct tatctcagcg cgaagaaaat
    32161 gagcctgcat cagaacagtt tctccgaagt gctggcgctg gcagatatcc gcggacatct
    32221 gccagcacat attgccctcg tcgggctgca acccgcgatg ctcgacgact acggcggcag
    32281 cctgagcgaa ctggcacggg agcaactgct cgctgcggaa caatcggcgc tggcgcagtt
    32341 ggctgcgtgg ggaagtgtgc cgcaactggc taatgaatcg cgttgtctca attatgactg
    32401 tctgtcgatg gaaaattacg aaggcgttcg cttgcgccag taccggatga cacaggagga
    32461 gcagggatga gcaacgacac gccatttgat gcgttgtggc aacgaatgct ggcgcgcggc
    32521 tggacgccgg tcagtgaatc ccgtcttgac gactggctta cgcaagcgcc agacggcgtg
    32581 gtgttattaa gcagtgaccc gaaacgcacg ccagaggtca gcgataatcc ggtaatgatt
    32641 ggcgaattac tgcacgagtt tcccgactat acatggcagg tggcgattgc tgaccttgag
    32701 cagagcgaag ccatcggcga tcgctttggc gtctttcgct ttcctaccac tttagtgttt
    32761 accggcggaa actatcgcgg cgtgctgaat ggtattcacc cgtgggcgga actgataaac
    32821 ctgatgcgcg ggcttgtcga accgcagcag gagcgtgcct catgagcgaa acttttttcc
    32881 atctgctggg gccaggaacg caaccgaacg atgacagttt cagcatgaat ccactgccga
    32941 tcacctgtca ggtgaatgat gaaccgagta tggcggccct ggagcaatgt gctcacagcc
    33001 cgcaggtgat tgcgctgtta aacgagttac aacatcaact aagcgaacgc cagccgccgt
    33061 tgggcgaggt gctggcagtc gatctgttaa atctcaacgc cgacgatcgt cactttatca
    33121 atacgcttct cggggaaggg gaagtgtcag tgcgcattca gcaggctgac gacagtaaaa
    33181 gtgaaataca ggaggcgatc ttctgcggat tatggcgggt gcgcagacgt cgcggcgaaa
    33241 agttgctgga ggacaaactg gaggctggct gcgcgccgct ggcgttgtgg caggcggcaa
    33301 cgcaaaatct cttgccgaca gattcgctgt taccgccgcc cattgatggc ctgatgaatg
    33361 gcctaccgtt ggcgcatgag ttactggcac atgtacgtaa ccccgacgcg cagccgcaca
    33421 gcattaatct gacgcaatta cccatcagcg aggctgatcg gctttttctc tcacgtctct
    33481 gtgggccggg aaatattcag attcgtacca ttggctatgg cgagagctat atcaacgcca
    33541 cggggttacg ccatgtctgg catttacgct gtacggacac cttaaaaggc ccgttactgg
    33601 aaagttatga aatctgccca ataccggaag tggtgctggc agcgccagaa gatttggtcg
    33661 actctgcgca gcggcttagc gaggtatgtc agtggctggc ggaagctgca ccgacgtaaa
    33721 aagacggtaa gtatcgcttt cagtcttatg aatatcgcaa tcggcgaata cctctggtcg
    33781 tagagtttca ggataaagag ggagatctaa cattatcggg ttatttttct ctcttcgcct
    33841 acaggagtgc gcatgtggga tgtcattgat ttatcgcgct ggcagtttgc tctgaccgcg
    33901 ctgtatcact ttttatttgt accccttacc ctggggctga tttttttgct ggctatcatg
    33961 gaaaccattt acgtggtcac cggcaaaaca atctaccgcg atatgacgcg cttctggggt
    34021 aagctcttcg gtatcaattt tgctcttggc gtggctaccg gcctgaccat ggagtttcag
    34081 tttggtacta actgggcatt ctattccaac tatgtgggcg atatttttgg cgcaccgctg
    34141 gcgatggaag cattaatggc cttcttcctc gaatccacct ttgtcgggct tttcttcttc
    34201 ggctggcagc ggctgaataa ataccagcac ctgctggtga cgtggctggt ggcgttcggt
    34261 tcaaatctct ctgcattgtg gatattgaat gccaacggtt ggatgcaata cccgaccggt
    34321 gcgcattttg atatcgacac cctacgtatg gagatgacca gcttcagcga gctggtcttt
    34381 aacccggtca gccaggtgaa atttgtgcac accgtaatgg cgggctacgt gaccggggcc
    34441 atgtttatta tggcgatcag cgcctggtat ttactgcgcg gacgggagcg cgatgttgca
    34501 ttacgctcgt ttgccatcgg ttccgtcttc ggtactctgg cgattatcgg cacgctacaa
    34561 ctcggggaca gttctgcgta tgaagtcgcg caagtacaac cggtaaaact ggcggcgatg
    34621 gaaggggagt ggcaaacgga acctgctcct gcaccgttcc atgtggttgc ctggccggaa
    34681 caggatcaag agcgtaacgc ctttgccatc aaaattcccg cgctgttagg gatcctcgcc
    34741 actcactcat tagataaacc cgtgccgggt ctgaagaatt tgatggctga aacctaccca
    34801 cgcttgcaac gcggacgtat ggcctggctg ttaatgcagg aaatatcgca aggcaatcgt
    34861 gagccgcatg tgttgcaggc attccgggaa ctggaaggtg acctgggcta cggcatgttg
    34921 ctctcccgct atgcgccgga tatgaatcat gtcacagccg cacagtacca ggcggcgatg
    34981 cgtggcgcga tacctcaggt tgcgccggta ttctggagtt tccgcatcat ggtgggctgt
    35041 ggttccctgc tgctactggt gatgctgatt gcgcttgtcc agacgctgcg tggcaaaatc
    35101 gaccagcatc gctgggtgct gaaaatggcg ctctggagtt tgccgttgcc gtggattgcg
    35161 attgaagccg ggtggtttat gaccgagttt ggtcgtcagc cgtgggcgat acaggacatc
    35221 ttaccgacat actccgcgca ctccgcttta accacaggac aactggcttt ctcactgatc
    35281 atgatcgtag ggctttacac cctgttctta atcgccgaag tctacctgat gcagaaatat
    35341 gcccgtctgg ggccgagcgc gatgcagagt gaacaaccga cgcagcaaca ggggtaaagg
    35401 agaaaatcat gtttgattat gaaacattgc gcttcatctg gtggctgctg attggcgtga
    35461 tcctggtggt ctttatgatc tccgacggat ttgacatggg gatcggctgt ctgctgccgc
    35521 tggtggcgcg taatgatgat gaacgccgga tagtgataaa cagcgttggt gcacactggg
    35581 aaggcaacca ggtctggttg atcctcgctg gtggggcatt atttgccgcc tggcccagag
    35641 tgtatgcagc ggcgttttcc ggcttttatg tggcgatgat cctggtgctg tgctcactgt
    35701 tcttccgccc gctggccttt gattatcgcg gaaaaatcgc cgatgcacgc tggcgtaaaa
    35761 tgtgggacgc cggtctggtc atcggcagtc tggtgccgcc ggtagtcttc ggtatcgcct
    35821 tcggcaactt gttgctcggc gtgccgtttg ccttcacacc gcaattacgc gtggagtatc
    35881 tcggcagctt ctggcaactg ctgacgccat tccctttatt gtgcggattg ctcagccttg
    35941 ggatggtaat tttgcaaggt ggcgtctggt tacaactgaa aactgttggt gtgattcatc
    36001 tgagttcaca gctggccacc aaacgcgctg cactgttggt gatgctgtgc tttttgctgg
    36061 cgggttactg gctgtgggtc ggtattgatg gctttgtact gctcgcccag gatgctaacg
    36121 gtccttccaa tccgttaatg aaactggtgg cagtgctacc tggtgcctgg atgaataatt
    36181 tcgtcgaatc gcccgttttg tggatcttcc cgctgctggg gttcttctgc ccattgctga
    36241 cggtgatggc gatttatcgt ggtcgcccag gttggggatt tttaatggcg tcattgatgc
    36301 aatttggcgt gattttcacg gcaggcatca cgctgttccc cttcgtcatg ccgtcaagcg
    36361 tgagtccgat ctccagcctg acgttgtggg acagcacttc cagccagctg acgctgagca
    36421 ttatgttggt aatcgtgctg atatttttgc ccattgtgtt gctctacact ctctggagct
    36481 actacaaaat gtgggggcgc atgacaacag aaactctccg ccgtaacgaa aacgagttgt
    36541 actaaggagc aggaacaatg tggtatttac tttggttcgt cggcattttg ttgatgtgtt
    36601 cgctctccac ccttgtgttg gtatggctgg acccgcgtct gaaaagttaa cgaacgtagg
    36661 cctgatgcgg cgcattagca tcgcatcagg caatcaataa tgtcagatat gaaaagcgga
    36721 aacatatcga tgaaagcgat cttaatccca tttttatctc ttctgattcc gttaacctcg
    36781 caatctgcat tcgctcagag tgagccggag ctgaagctgg aaagtgtggt gattgtcagt
    36841 cgtcatggtg tgcgtgctcc aaccaaggcc acgcaactga tgcaggatgt caccccagac
    36901 gcatggccaa cctggccggt aaaactgggt tggctgacac cgcgcggtgg tgagctaatc
    36961 gcctatttcg gacattacca acgccagcgt ctggtggccg acggattgct ggcaaaaaag
    37021 ggctgtccgc agcctggtca ggtcgcgatt attgctgatg tcgacgagcg tacccgtaaa
    37081 acaggcgaag ccttcgccgc cgggctggca cctgactgtg caataaccgt acatacccag
    37141 gcagatacgt ccagtcccga tccgttattt aatcctctaa aaactggcgt ttgccaactg
    37201 gataatgtga acgtgactga cgcgatcctc agcagggcag gagggtcaat tgctgacttt
    37261 accgggcatc ggcaaacggc gtttcgcgaa ctggaacggg tgcttaattt tccgcaatca
    37321 aacttgtgcc ttaaacgtga gaaacaggac gaaagctgtt cattaacgca ggcattacca
    37381 tcggaactca aggtgagcgc cgacaatgtc tcattaaccg gtgcggtaag cctcgcatca
    37441 atgctgacgg agatatttct cctgcaacaa gcacagggaa tgccagagcc ggggtgggga
    37501 aggatcaccg attcacacca gtggaacacc ttgctaagtt tgcataacgc gcaattttat
    37561 ttgctacaac gcacgccaga ggttgcccgc agccgcgcca ccccgttatt ggatttgatc
    37621 atggcagcgt tgacgcccca tccaccgcaa aaacaggcgt atggtgtgac attacccact
    37681 tcagtactgt ttattgccgg acacgatact aatctggcaa atctcggcgg cgcactggag
    37741 ctcaactgga cgcttcccgg tcagccggat aacacgccgc caggtggtga actggtgttt
    37801 gaacgctggc gtcggctaag cgataacagc cagtggattc aggtttcgct ggtcttccag
    37861 actttacagc agatgcgtga taaaacgccg ctgtcattaa atacgccgcc cggagaggtg
    37921 aaactgaccc tggcaggatg tgaagagcga aatgcgcagg gcatgtgttc gttggcaggt
    37981 tttacgcaaa tcgtgaatga agcacgcata ccggcgtgca gtttgtaatg cataaaaaag
    38041 agcattcagt tacctgaatg ctctgaggct gatgacaaac gaagaactgt ctaatgcgta
    38101 gaccggaaaa ggcgttcacg ccgcatccgg ccactttcag ttttactctt tctcggagta
    38161 actataaccg taatagttat agccgtaact gtaagcggtg ctggcgcgtt taatcacacc
    38221 attgaggata gcgcctttaa tattgacgcc tgcctgttcc agacgctgca ttgacaaact
    38281 cacctctttg gcggtgttca agccaaaacg cgcaaccagc aggctggtgc caacagaacg
    38341 ccccacgacc gcggcatcac tcaccgccag catcggcggc gtatcgacaa tcaccagatc
    38401 gtaatggtcg ttcgcccatt ccagtaattg acgcatccga tcgcgcatca gcagttcaga
    38461 cgggttaggt ggcacctgac cgcgagtaat cacatcaaag cctcctttgc caaaatgctg
    38521 gatcactttg ttgagctcat ctttacctgc cagatattcc gacaagccat gttcattact
    38581 cacggtaaac aggttatgcg aataaccacg gcgtaagtcg gcatcaataa ataacacttt
    38641 ttgatcggac tgggcgatca ccgctgccag agttgaactg acaaacgttt taccactgtc
    38701 tggcgtcgca ccggtgatca tcagaatgtt attctccgtc tccatcatag cgaagtgcag
    38761 actggttcgt agcgcacgta cggcttccac agcagaatcc gccgggttat ccaccgccag
    38821 gaaggggata tttttagtac gatggcgctg ctgattagaa aataaatttt tcttacgcag
    38881 acgggtacgt ttatccagcc actcggacat tgggatagtg gcataaacgc tgatgccgtg
    38941 ctcttccagt tgttccgggg cttctacacc acgacgcaac atcgcacgcg ccagcacggc
    39001 acccaccgaa ataaacaggc caagaataaa accaagcacc acgttcaacg cttttttcgg
    39061 tttcactggc tgcggctgag tgactgctgg gtcgataatc cgcacgttac caatggcact
    39121 ggatttcgaa atactcaact cctgctggcg gttaagtaat tgcagatata ccgcacggcc
    39181 cgcttctacg tcacggctta aacgtaacac ttcctgttga gtggaaggca ttgccgatac
    39241 ccgcttattc aggcgtttgc gttcttgctc cagcgtctgg cgtttttcca gcagcgcacg
    39301 ataagttggg tgatctttct tatacagctg ggagatctct gcctcgcgga aagtcagctc
    39361 attgagttga ttatcaacgt tcacaatctg ctcaagaacg gctttggctt ctaggttaag
    39421 gtcaaccgaa tcgcgctgct ggcgataaac gttgagtttt tcttccgcct ggtccagctc
    39481 gctgcgcact tcaggcaact ggcgctgtaa gaattcaagg ctttgtgaat cctgcgccgc
    39541 ctggcgagcg atattctgtt gcaaatagtt gttagcgatg ctgttcagaa tacgagtaat
    39601 caactgggga tcatcaccag tcatggtaag ttccagcatc ccgctttctt tactgcgttc
    39661 gctaacggta aaggtttcct gcaatgcgtt aatcgcttcc agttcggtac gctggctcag
    39721 gacaaactgt gttcctggtt tggccttaat gtccgcgata gtcagcgcaa cgccatcttt
    39781 ttccagacgc tggccgacca taccattgac ggtgaactct tcaccttcca gtgtatagtg
    39841 gccgttttcc ccaaccgtga gtgtcagttg ctgatcctga ccattcagtt gtggaatatg
    39901 catccagctg atcgccagct cacctggttt ttctttggtt aatctcgccc agccgcgacc
    39961 cacaatcgga aaatacttct gctcaactat gtcgtgcaga ttcagttcag caatggtttt
    40021 accgagaatc atgcgcgatt gcagcagttg gatctccggt gcagactcgg gcgatgagtt
    40081 agggatcata tcgctcaggc cgctgagaat ggcgttgccc tgtttttgct caacctggac
    40141 cagagtatct gcctgataaa ttggtgtgct taacagcgag taagcgacag cgatcagcgt
    40201 gaataacgcg gttacgctga taataaactt acggtgatcc cataactcgc cgaccagacg
    40261 aagcagatcg atctcatttt cctgagtgct gcctggtggc gtattcatat ttttagttgt
    40321 catcgtattt tataaactca aattaccggc tgaggcgctt cgcccattcc tgactggcgc
    40381 gctccaacat accgtagaca tgttcaaatg cgtcctgact tttacgatag ggatccggga
    40441 tctctttctg ttccagccat tgcccaaaca gcattgtctt cccgcggacc tcgggagcga
    40501 ttgcggtaac ctgagcaata tgctctgact ccattgccag aatcagatcg taatttcgtg
    40561 ccatctcagc agtgagctta cgtccggcat gtccttcaag agatacgcca tgattagcgg
    40621 caacatctgc tgctgtcgca tctgcagggt gttttaccag accatgaaca cctgccgatt
    40681 tgacttttac gcctggcaga cgtttacgca gcaagcgctc gccaatgggc gaacggcaaa
    40741 tgttgccggt acaaaccacc aggattgagt taaattttag ttgggccatg tttgaatcca
    40801 ccgtgaagtc tctgtcaggt catgcacccc ggaaatcgtc ggtaccagca gagaaatcac
    40861 acggttccaa cgagccagcg gcgcggtagt gacataaaca atgtcgtaag gttcaagctg
    40921 gaattccgtt ccgagtatca ttgccgatgc gtccttcgca ttcagctgat aaatattggc
    40981 gattttgcca ttctgcttgt tttgcgttgc acgaataaca aaaatccccg tcgcatcagc
    41041 gacatcctga ttcataccct cagcgttacc cagcgcttcc gccagggtca taccgctgcg
    41101 gtccattttc agcgtgctct gtttacccac ttcacccatc acgaagactt tcagatcatc
    41161 gttgcgcgga ataaataaaa tatcgcccgg gtgcagcagt ttgttctggc gcaagtcgcc
    41221 acgctgcatc agcgcataga ggttcacttt ggtctttacg ccattctggg tgagcaccac
    41281 gttgcgccag tctgcatctg cggtcagacc gcccgcggcg ttaatcgcat ccatgatggt
    41341 cagaggaata ttggtaatcg gctgctgacc cgatttgctg acttcgccgg tgacgtacgc
    41401 tttttgcgat cggaacgccg cgacgctgac atcgacttgc gggctttcaa tgactgaatc
    41461 gagacgcgct gtaatctcgt tgcgtacctg ggtaagtgtt ttcccggcca ctttcaggcg
    41521 accaatatag gggtagaaaa tcgcaccatc ggcgttaacc cagttgccgg tatcactggc
    41581 gctacggtat tgccctgcgg gggtggttag ttccgggtga tcccagaccg taaccatcaa
    41641 cacatcacca atcccgatac gatattcata gtttgcgatt tgctgctcca gctccgggtt
    41701 agcctgcgac ataatcggtt tggcgcgcaa ctgatcaatc aatcctggcg tgacgggata
    41761 gatattgacc attttgtcca ggtcatactg attgtcaggt agttcaatga catccttgtt
    41821 cctggtcgat aaattctgac caggaacaag ggtacatgct gtcaaagaca gaacagccaa
    41881 tgtcagtacc gaaaacttaa aaatgttctt tttcattggt ttatatttat cagtcaatgt
    41941 aatgttatga attacgtcag gttattgtcc aaccggtact tctcgctctg ccgtcattgg
    42001 atagagctgg tatttgcgtg acagcatttg tccaccatca cgcgtcaatg gtgtccacga
    42061 aaccaccgcg cggttgcggt taggtccaat ggtcatcaag tcgaatggaa tcgagatata
    42121 aaaaccttta ctaaagccgc cttcgccgta gtcatcttta gatacgttac tgattgccgc
    42181 ccatacccct accgccacgc cgctgtcaaa gcgtttggcg acgtcgatcg ttgctccttt
    42241 atcttttgcc agatattgcc caacgctaag tttcatcagt acgccattga gcgtcggcgg
    42301 gttccagtaa gccgtcacga aaccagttgg cgtggaataa tcggtgaagc gcatcatgtt
    42361 gtcccagtca cgttgcttca cgtagttaac gtccacaccc agcgcccagc tggcatctag
    42421 cgggcgataa agcagctcgg aaccgacacc ggcgtacatc gtttccagat aaccgccata
    42481 cacctggcca tagaaaccat tgcctaagtc ggcaaagtag ttcgcctgca agttgttgag
    42541 ataaacgtca ttgcgaacgt aatcacggat atgcgtgcgc acgcggggca gggtagagtc
    42601 cgcgggcaac agcgaagact taaacttgtc gtagttgttg taaatattgg tgaaaatacc
    42661 gccatcaagc agcaggtggt cggtaaacca gtagcgggca ctggacatca gccccagctg
    42721 gaacatatag aaatcttccg ggccgcccag cgactgtgaa agtgttgggt tgaaagagta
    42781 gctaaagcga tcttcacgaa tacggtagcc ccgaccaaag gcagaaagat cttctgcttc
    42841 aacacgttgt tgttgaagtt gctctgattg accaggcgct gtaccagcca gctgtttgcg
    42901 caggctggct acgtcggttt cggtagtcac catcgccata tgctcgcgct tttgcgtcac
    42961 gctaatcttc tcaacgcctt gcggcaggtt attcaccaga atccggttgg cacgatcgac
    43021 cgcttcgcga gaatcacggt atttgtattg ttgaccagac atatacagcg tcttatcgcg
    43081 cagctgaatt tccggtgctt caaaacctgc gttgtacttc agcgcggtaa gttggttcgc
    43141 tactgtggtg tactgcaatc cttcagattc aggcgcaggt tgatatgccg gttttggcgt
    43201 atcgcgcagg gcagggcgca gatcgttgaa attggtccgt aacgtgaagc caaacatcaa
    43261 cgtgttaccg cgttcataac tcaggttgag atctgcccag ctggcagcgc gataaactgc
    43321 gccgacgttg aaatggcttg cctgaggcag tttgccagcg aaatcattct ggtaattgtt
    43381 gccatcgtat tcgagtttca gacgcagggg attccacggc gtttgatact caatgccgcc
    43441 aaagatggaa gccgggccac gaaagatatc gctaaagctg atatcgcccg catcgtgaga
    43501 ctctgcgcga tgacaatatt tatcgttcac ccggcaaaac gggttggtaa tattgcccgc
    43561 attaccggcg tagccccatg ccatcccgag ggtgaaatca aatggccccg cttgcttgct
    43621 ggccaccaga tactcaccgt caaacagacc cgtaccagca atatcacgtt taccaaacgc
    43681 cacttgcggt agccagtaac cttcttccca caggcgaagt ttaaaatcga atgatttgtc
    43741 tttgtatgac tgatcgccgc tgaaatcttc ccactggcta tatttgcgtg tgcgcacatc
    43801 cgtataacga atggtgcctt ccagccatgg gaacagcgcc acggaggtgg agtagaaccg
    43861 gtattgatcg ttatcccggt agttgacgct gaattcacct tccggtgcga tgcgcgcatt
    43921 tggcatctgc aacaatcctg tgccgccaaa gtctgattgc gacgaaccca gcggatccgg
    43981 gtaggttaat acttcagcat gacaggcact ggagacggca atggccaggc agcttaaaag
    44041 ataagaattc ttcttcatca gtcaggaaca cgttgcgtta aaacagacac aatctggtca
    44101 tttaaatcag catatttttc gggcagtaca tgcgcagaaa agcccaacca aagctggctg
    44161 ccaggcggtg gttccacatg tcttttattc cacagggcaa caggggcgac gacagtctca
    44221 ccctcaggcg taatcaccat aacgttattc ttgtccgcgc ctgcaaggcg ggggtgatct
    44281 tgcagataat cggtaacgct acggcctgcc tgccagggca attgcccggc cccagaaacc
    44341 gcacccagca gagtaatagt caccggacgc tgtacggtat agagcgtata gtccccgacc
    44401 aacgggggat tgctgttctc atccactcga acaaaatcag ggtccagttt gaccggtaag
    44461 cgaccagtga tattgagatt gagtaactgc tggcgcacag atttaatcgt tgccgccaca
    44521 tcgtcgtcag cttctgcttc ccaggaagca agctgtgcca tgacatgttg gtagtcttta
    44581 agcgctttag ctttcgccgc agagtcggtc agcaaggcac caggccacca gaggcgatcg
    44641 cgcagttgcg gttgtgtcac caactggaca acattttcca tggggccaac ggaaagtgtc
    44701 tgttgttcgc caggcagata aatagtcacc gttccttgcg caaaggcatg gggtgtcatt
    44761 acgtaaagta cgctggcaat gaaatacgac tgtaatttat tcatggtgcg ggtttaagaa
    44821 acgtcatttc tacgggaata acccctgcgc ctaacatttg ccgactttga cgaacctggc
    44881 cagtagccga atcgacccag aatgtgtttt gccactgttt ttctggccgc gtggaagtca
    44941 cctcttcctg ccagacatta cacagcacat tgcgacccgc aatattcagc gtctctaatc
    45001 cagcaaagga aaaagtagaa ctcagggtcg cagagcgaaa atggttgtct tcactccaga
    45061 gaatatcgcg cgtccagcgg ctgccatctt ttattgccag cgcgttacgc agagggtcct
    45121 gcccggaatt tgtcacttcc agcagattat tattaagttt gacggttttt aaaagacgtc
    45181 cattgtgggt aaccagcatg gcgttatcct gggataacca tttgctttgt tcttgttcta
    45241 tatatcccag taccacaaag attcgttgcc cttcattaag gcgtaaatac atcgtggaat
    45301 aaggcaacgc ctggatctgc tgatccgcta cggtgatatc ctgattatcg aaaaggctgg
    45361 cgcgaaatgt atcgaccata ctttgctggc tgtgcgtaca tcccgcaaga aatagtgcga
    45421 aaatcgataa aataagaggg cgcaccttta atccttgaaa ggttaattaa aaaaatgcaa
    45481 atctttaaag cccggtacga atatcgcaac cgggtatcat ttaactttaa tttcgacgcg
    45541 ttaaagcagt attagcgtgt agtggacgtg gtggttgagg tgttatgaga accgccatcg
    45601 ccactgtcat ttgctgcaac aactgccaac gtcgccacac caacagccgt tgccacaccg
    45661 acaactgtcg ccgtgccgac agcgccagca gccacgccac cagtggtagt agcagtcgtg
    45721 gttgcgccgg tagtggtcgt tgtggtgcca gtagtacccg tcgttgcctc ggtcccgttt
    45781 gttgcttcag gggcagcaaa cgcagctggc gtcgtcaaca tgaaggccat aaggattgca
    45841 gaaagcttgt gtttcataac ttttccttta ttcatcgcat ggacaatacg tacgcttgag
    45901 atcaggattg tctactttga gtataacatc cttgtgaaat ctccctttcc tcataccgct
    45961 tgcactatat actgcgggta ggaaaggcgc gcagagggaa atataagatt gttaactatc
    46021 cccctctgga aatacgactt gtgagcataa gataaatctt agcaaaatac aaattaaaga
    46081 taaggaaatt gttacgaaag ctattaatta ttgctggtaa tatagtttca agtggtacta
    46141 taactggctg ttaaatggca tttataacta ttaggtgcag agatattcgc ttaaatggat
    46201 tagtttcttt gtggaaaagt aactgatgtt ataatatatg atgttgacta ttgcactatt
    46261 ccaataaaac cagtcagctt taaacaagca gcgtcatatt aagagagata aacatttgcc
    46321 gctgttggtc ctcgcaggcc atttacgcgg caaaattcca cacgtaatcc tggtataagc
    46381 acttctgcgt cgcggggagt gaatgcggaa atatggacct gaacttcttt acgaccgtcg
    46441 gaggggataa tgaatccttt gccgcttttg cgatcaaagg ttttgacaat tcctgtcatt
    46501 ttacgggaca aacaaattcc ttactgaaaa tactgcgctg cactatacgg ggttaataaa
    46561 ataaagccag cgatatttaa gaccgccgga cggctaaaat aaaatttgct taatctcaat
    46621 tatcatgcgt taatagctgc gtcggtttga aagacagaca gcatacaaag tagtttacta
    46681 aagcagttct cattatcagg cattatcccc ttcttttgag tctctctcct gaacactaag
    46741 tagtttctgt attaaagccc tgtttgccga aaggcccaaa atgaaggaag taaaatatgt
    46801 ctaataaaat gactggttta gtaaaatggt ttaacgcaga taaaggtttt ggctttatca
    46861 ctcctgatga tggcagcaaa gacgttttcg tccatttcac tgccatccag agcaatgaat
    46921 tccgcacgct gaacgaaaat cagaaagttg aattttctat tgagcagggg caacgtggcc
    46981 ccgcggcagc gaacgttgtt acgctctaag gttgccgtta ttactcaaca tctccatttc
    47041 cgctgtccat gttgtcatgg ttcacagtac cgcacatcgg cattcgatgt gacggagcga
    47101 aatcctttgg gcgctaagtg tattttttgt aaatcgacga tgatcacctt tgataacgtc
    47161 acgctgcaaa tacgcactga ccatgcgccg ctggatttca caaaataata tcaggctccc
    47221 tcg
//
LOCUS       DAJTMC010000008        43879 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.008, whole genome shotgun sequence.
ACCESSION   DAJTMC010000008 DAJTMC010000000
VERSION     DAJTMC010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43879
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.008"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            83..607
                     /gene="lpfE"
                     /locus_tag="OO130_000443"
     CDS             83..607
                     /gene="lpfE"
                     /locus_tag="OO130_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000792957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial protein LpfE"
                     /protein_id="HCR5363976.1"
                     /translation="MKRTILALCLLPLSFSSFAATTDLGPKGTLKFTLKISQGACELV
                     KDSVEVDMGTAVLKKPVRIGTEINPTPFSIGLKNCSEVVRAYVTMDGTPNADDSNLFA
                     LDAGGATGVGLKIKTAAGVQQFPENTTPTPVEFAINGDGAHQLNYIASYVPIRADATI
                     GRADATVDFTVQYE"
     gene            860..2551
                     /gene="eptB"
                     /locus_tag="OO130_000444"
     CDS             860..2551
                     /gene="eptB"
                     /locus_tag="OO130_000444"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HCR5363977.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQAKEAAAN"
     gene            2643..2719
                     /locus_tag="OO130_000445"
     tRNA            2643..2719
                     /locus_tag="OO130_000445"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2677..2679,aa:Pro,seq:cgg)
     gene            3463..5070
                     /gene="dppA"
                     /locus_tag="OO130_000446"
                     /pseudo
     CDS             3463..5070
                     /gene="dppA"
                     /locus_tag="OO130_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
     gene            5378..6397
                     /gene="dppB"
                     /locus_tag="OO130_000447"
     CDS             5378..6397
                     /gene="dppB"
                     /locus_tag="OO130_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HCR5363978.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPASGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            6407..7309
                     /gene="dppC"
                     /locus_tag="OO130_000448"
     CDS             6407..7309
                     /gene="dppC"
                     /locus_tag="OO130_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HCR5363979.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVVIVLF
                     IAIFANWIAPYNPAEQFRDALLAPPAWQEGGSMAHLLGTDDVGRDMLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            7320..8303
                     /gene="dppD"
                     /locus_tag="OO130_000449"
     CDS             7320..8303
                     /gene="dppD"
                     /locus_tag="OO130_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HCR5363980.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLVFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            8300..9304
                     /gene="dppF"
                     /locus_tag="OO130_000450"
     CDS             8300..9304
                     /gene="dppF"
                     /locus_tag="OO130_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HCR5363981.1"
                     /translation="MSTQEATLQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLSGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(9334..10605)
                     /locus_tag="OO130_000451"
     CDS             complement(9334..10605)
                     /locus_tag="OO130_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCR5363982.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            11081..11188
                     /locus_tag="OO130_000452"
     CDS             11081..11188
                     /locus_tag="OO130_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCR5363983.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(11275..12954)
                     /gene="bcsG"
                     /locus_tag="OO130_000453"
     CDS             complement(11275..12954)
                     /gene="bcsG"
                     /locus_tag="OO130_000453"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HCR5363984.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVETTGGAPVVGDMPAQTTPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTTVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(12951..13142)
                     /gene="bcsF"
                     /locus_tag="OO130_000454"
     CDS             complement(12951..13142)
                     /gene="bcsF"
                     /locus_tag="OO130_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HCR5363985.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(13139..14710)
                     /gene="bcsE"
                     /locus_tag="OO130_000455"
     CDS             complement(13139..14710)
                     /gene="bcsE"
                     /locus_tag="OO130_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HCR5363986.1"
                     /translation="MRDIVGPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDYQGPEKIKLFSMLNHEKGLYYLTRDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLTHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            14983..15171
                     /locus_tag="OO130_000456"
     CDS             14983..15171
                     /locus_tag="OO130_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjR family protein"
                     /protein_id="HCR5363987.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            15183..15935
                     /gene="bcsQ"
                     /locus_tag="OO130_000457"
     CDS             15183..15935
                     /gene="bcsQ"
                     /locus_tag="OO130_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HCR5363988.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHRQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCDHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            15932..18550
                     /gene="bcsA"
                     /locus_tag="OO130_000458"
     CDS             15932..18550
                     /gene="bcsA"
                     /locus_tag="OO130_000458"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HCR5363989.1"
                     /translation="MSILTRWLLIPPVNARLIGGYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYPHINASRPRPLDPVRYLIQTCWLLIGTSRKETPKPR
                     RRAFSGLQNIRGRYHQWMNELPERVSHKTQHLDEKKELGHLSAGARRLILGIIVTFSL
                     ILALICVTQPFNPLAQFIFLMLLWGVALIVRRMPGRFSALMLIVLSLTVSCRYIWWRY
                     TSTLNWDDPVSLVCGLILLFAETYAWIVLVLGYFQVVWPLNRQPVPLPKDMSLWPSVD
                     IFVPTYNEDLNVVKNTIYASLGIDWPKDKLNIWILDDGGREEFRQFAQNVGVKYIART
                     THEHAKAGNINNALKYAKGEFVSIFDCDHVPTRSFLQMTMGWFLKEKQLAMMQTPHHF
                     FSPDPFERNLGRFRKTPNEGTLFYGLVQDGNDMWDATFFCGSCAVIRRKPLDEIGGIA
                     VETVTEDAHTSLRLHRRGYTSAYMRIPQAAGLATESLSAHIGQRIRWARGMVQIFRLD
                     NPLTGKGLKFAQRLCYVNAMFHFLSGIPRLIFLTAPLAFLLLHAYIIYAPALMIALFV
                     LPHMIHASLTNSKIQGKYRHSFWSEIYETVLAWYIAPPTLVALINPHKGKFNVTAKGG
                     LVEEEYVDWVISRPYIFLVLLNLVGVAVGIWRYFYGPPTEMLTVVVSMVWVFYNLIVL
                     GGAVAVSVESKQVRRSHRVEMTMPAAIAREDGHLFSCTVQDFSDGGLGIKINGQAQIL
                     EGQKVNLLLKRGQQEYVFPTQVARVMGNEVGLKLMPLTTQQHIDFVQCTFARADTWAL
                     WQDSYPEDKPLESLLDILKLGFRGYRHLAEFAPSSVKGIFRVLTSLVSWVVSFILRRP
                     ERSETAQPSDQALAQQ"
     gene            18561..20899
                     /gene="bcsB"
                     /locus_tag="OO130_000459"
                     /pseudo
     CDS             18561..20899
                     /gene="bcsB"
                     /locus_tag="OO130_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
     gene            20906..22012
                     /gene="bcsZ"
                     /locus_tag="OO130_000460"
     CDS             20906..22012
                     /gene="bcsZ"
                     /locus_tag="OO130_000460"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HCR5363990.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            21994..25467
                     /gene="bcsC"
                     /locus_tag="OO130_000461"
                     /pseudo
     CDS             21994..25467
                     /gene="bcsC"
                     /locus_tag="OO130_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsC"
     gene            25549..27537
                     /gene="hmsP"
                     /locus_tag="OO130_000462"
     CDS             25549..27537
                     /gene="hmsP"
                     /locus_tag="OO130_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HCR5363991.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            27720..29006
                     /gene="dctA"
                     /locus_tag="OO130_000463"
     CDS             27720..29006
                     /gene="dctA"
                     /locus_tag="OO130_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HCR5363992.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPVSVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            29226..30722
                     /locus_tag="OO130_000464"
     CDS             29226..30722
                     /locus_tag="OO130_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCR5363993.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAAATSVATDDVHPEVTDIPPAQ"
     gene            complement(30818..31747)
                     /gene="kdgK"
                     /locus_tag="OO130_000465"
     CDS             complement(30818..31747)
                     /gene="kdgK"
                     /locus_tag="OO130_000465"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HCR5363994.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDISLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSTAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            31979..32746
                     /gene="pdeH"
                     /locus_tag="OO130_000466"
     CDS             31979..32746
                     /gene="pdeH"
                     /locus_tag="OO130_000466"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HCR5363995.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFIEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLGDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPIETLNTAVLAL"
     gene            32816..34876
                     /locus_tag="OO130_000467"
     CDS             32816..34876
                     /locus_tag="OO130_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCR5363996.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEQLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(35110..36432)
                     /locus_tag="OO130_000468"
     CDS             complement(35110..36432)
                     /locus_tag="OO130_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HCR5363997.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLLILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVSSI
                     LGASVAPYIAAWLQTNYGLGAVGLYLAAMAGLTLIALLLTHETQHQSL"
     gene            complement(36843..37856)
                     /gene="yhjD"
                     /locus_tag="OO130_000469"
     CDS             complement(36843..37856)
                     /gene="yhjD"
                     /locus_tag="OO130_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HCR5363998.1"
                     /translation="MTQENEIKRPTQDLELEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMLGK
                     TQP"
     gene            complement(37905..38804)
                     /locus_tag="OO130_000470"
     CDS             complement(37905..38804)
                     /locus_tag="OO130_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5363999.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLNELDGLFQQDATSISGKLRIDIPPGI
                     AKNLLLPRLSEFLYLHPGIELELSSHDRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            39324..39926
                     /locus_tag="OO130_000471"
     CDS             39324..39926
                     /locus_tag="OO130_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR5364000.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
     gene            complement(39977..41626)
                     /locus_tag="OO130_000472"
     CDS             complement(39977..41626)
                     /locus_tag="OO130_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HCR5364001.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVKRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKLI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
     gene            42030..43427
                     /gene="ccp"
                     /locus_tag="OO130_000473"
     CDS             42030..43427
                     /gene="ccp"
                     /locus_tag="OO130_000473"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HCR5364002.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            43638..>43879
                     /locus_tag="OO130_000474"
     CDS             43638..>43879
                     /locus_tag="OO130_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR5364003.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSI"
BASE COUNT        10539 a        11000 c        11926 g        10414 t
ORIGIN      
        1 ccgttagcac taccggaaaa ttacccgctg ccggggatta tagcggtatt gctaccatac
       61 atgtcgaact ggagtaacag tcatgaagcg aacaatactt gcgttatgcc tgctgcctct
      121 ttccttttcg tcattcgcgg caaccacgga tttagggccg aaaggaacat tgaaattcac
      181 ccttaaaatc agccagggtg cttgcgaact ggtgaaagac agcgttgagg ttgacatggg
      241 caccgcggtt ctcaaaaagc cggtaagaat cggaacggaa attaatccca ctcctttttc
      301 catcggcctg aaaaactgta gcgaagtggt cagagcctac gtgactatgg acggtacacc
      361 caatgcagac gactcaaatc tttttgcgct ggatgcgggt ggcgctacag gcgttgggct
      421 gaaaatcaaa accgctgctg gagtacaaca gttcccggaa aatacaacgc cgacaccggt
      481 tgagtttgca attaacggtg atggcgcaca tcaactgaac tatatcgcca gctacgtgcc
      541 tattcgcgcc gatgcaacta ttggtcgcgc agatgcaacg gtcgacttta ctgtacagta
      601 cgaataattt cacatcttaa gggccagtta actggccctt tttactgctt tttgtaacgt
      661 taaagtctct tttcaaactt gcatttttgt aaatttgtgc ttcatgcaca ctctttcccc
      721 acactttttc gctttgctgt ggtctactta ttcgcgcgtg tagattttac ttatctgact
      781 acctccgcac ttttcccctg ccgggcctga aaagccgcta agcagggtgt tatcacctgt
      841 ttgtccaggg tttgtttgca tgagatacat caaatcgatt acacagcaga agctgagctt
      901 tttgcttgca atctatattg gcctttttat gaatggcgcg gttttttatc gccgcttcgg
      961 cagctatgcg cacgatttta ccgtctggaa aggcgtttct gctgttgttg aactggccgc
     1021 caccgtactg gtgaccttct ttttactacg tcttctatcg ctgtttggcc gccgcagctg
     1081 gcgtattctg gcgtcgctgg tggtgctctt ttccgcaggt gccagctatt acatgacctt
     1141 ccttaatgtg gtcattggtt atggcatcat cgcttccgtc atgaccaccg atatcgacct
     1201 gtcaaaagaa gttgttggtc tgaactttat tctctggtta atcgccgtta gtgcattgcc
     1261 tcttatcctt atctggaata accgctgtcg ctacaccttg ctccgacaac tgcgaacccc
     1321 agggcagcgt attcgcagcc tggcggtcgt cgtactggcg ggtattatgg tttgggcacc
     1381 gattcgtttg ctggatatcc agcagaagaa agtggagagg gcgaccggcg ttgatttgcc
     1441 gagttatggc ggtgtcgtag cgaactctta tctgccatca aactggcttt ctgcgttggg
     1501 gctgtatgcc tgggcgcggg tcgatgaatc ttccgataat aattcattgc ttaatccggc
     1561 gaagaagttt acataccagg ctccacaaaa cgttgatgat acttatgtcg tgtttatcat
     1621 cggtgaaacc acgcgttggg accatatggg tattttcggc tatgagcgta ataccacgcc
     1681 gaaactggcc caggagaaaa atctggcggc gttccgtggt tactcctgtg ataccgcaac
     1741 caaactctca ctgcgttgca tgtttgtacg tcaggggggc gcggaagata atccgcagcg
     1801 cacattaaaa gaacagaaca ttttcgcggt tctgaagcag ttaggattca gttctgacct
     1861 ctacgctatg cagagcgaaa tgtggttcta cagcaacacg atggcggaca acattgctta
     1921 tcgtgagcag attggtgcgg agccacgtaa tcgtggcaag ccggtagatg atatgttgct
     1981 ggtagacgaa atgcagcaat cgctagggcg caacccggat ggtaagcatc tgatcattct
     2041 gcataccaaa ggttcgcatt ttaactacac ccagcgttat ccgcgtagct tcgcgcagtg
     2101 gaagccggaa tgtattggtg ttgatagcgg ctgtaccaaa gcgcagatga tcaactccta
     2161 tgacaactcg gtgacctatg tggatcactt tatctccagc gtgattgatc aggttcgcga
     2221 taagaaagcg attgtgttct acgcagctga ccacggtgag tcaattaatg aacgcgagca
     2281 cctgcacggc acgccgcgtg aattggcacc gccggagcag ttccgcgtac cgatgatggt
     2341 ctggatgtca gataaatatc tggaaaatcc ggccaatgcg caggcatttg cgcagctgaa
     2401 aaaagaagcc gacatgaaag tgccacgccg tcacgtagag ctgtacgata ccatcatggg
     2461 ttgtcttggc tatacttcac cggatggtgg aattaacgaa aacaacaact ggtgtcacat
     2521 cccgcaggca aaagaggcag cggctaacta agcggcctgc tgactttctc gccgatcaaa
     2581 aggcattttg ctattaaggg attgacgagg gcgtatctgc gcagtaagat gcgccccgca
     2641 ttcggtgatt ggcgcagcct ggtagcgcac ttcgttcggg acgaaggggt cggaggttcg
     2701 aatcctctat caccgaccaa attcgaaaag cctgctcagc gagcaggctt ttttgcattt
     2761 gaagcccatg aggatgagaa cctccggggg ctgggaggtt cgactcgacg taggcctgat
     2821 aagacgcgcc agcgtcgcat caggcaacgg caccgcactt attgtcggat gcggcgcgac
     2881 cgccttattc gaccaacaca ccacacattt tcctgccaac tttgcgcccc atatctcact
     2941 atccattaac ggatttgtga cagaaaccat tgtctttttt tatatttgct taaatctttt
     3001 tttgcgttac ttatggctgg cgctggcatt ttccgctgtg cgttttagcg ttcatggtat
     3061 taatcgctca gatatccacc ttttcctcag atttttttca ttaaatgcat aaaacttaat
     3121 caccaaatgt tgctaaatat taagctgatt gttctggtta tgttggagta gcacaaattt
     3181 ctctgctgca aatagctgtt tgaagttttt taatctttgt ttgtgggttc tcaccgttgg
     3241 tgtaaatccc ggttggctgt attgacgttt tcacattctg ttgacagatt gtaggtcacg
     3301 aggggcattt tatggaggat ccgcactgtt acactgatgt taattagtac ggcatcccca
     3361 cctcataacg ttgacccgac cgggcaaaaa acaaaaaagg tcaggtagcg acaacccact
     3421 gcaaagggtt aaaacaacaa acatcacaat tggagcagaa taatgcgtat ttccttgaaa
     3481 aagtcaggga tgctgaagct tggtctcagc ctggtggcta tgaccgtcgc agcaagtgtt
     3541 caggctaaaa ctctggttta ttgctcagaa ggctctccgg aagggtttaa cccgcagctg
     3601 tttacctccg gcaccaccta tgacgcctct tccgtaccgc tttataaccg cctggttgaa
     3661 tttaaaatcg gcaccactga agtgataccg ggcctcgctg aaaagtggga agtcagctaa
     3721 gacggtaaaa tctatacctt ccatctgcgt aaaggtgtga agtggcacga caataaagaa
     3781 ttcaaaccga cgcgtgaact gaacgccgat gacgtggtgt tctcgttcga ccgtcagaaa
     3841 aacgcgcaaa acccgtacca taaagtctct ggcggcagct acgaatactt cgaaggcatg
     3901 ggcttgccgg agctgatcag tgaagtgaaa aaggtggacg acaacacagt tcagttcgta
     3961 ctgactcgcc cggaagcgcc gttcctcgct gatctggcaa tggacttcgc ctctattctg
     4021 tcaaaagaat atgctgatgc gatgatgaaa gccggtacac cggaaaaact ggacctcaac
     4081 ccaatcggaa ccggtccgtt ccagttacag cagtaccaaa aagattcccg tatccgctac
     4141 aaagcgtttg atggctactg gggcaccaaa ccgcagatcg atacgctggt cttctctatt
     4201 acccctgatg cttccgtgcg ttacgcgaaa ttgcagaaga atgaatgcca ggtgatgccg
     4261 tacccgaacc cggcagatat cgctcgcatg aagcaggata aatccatcaa cctgatggaa
     4321 atgccggggc tgaacgtcgg ctacctctcg tataacgtgc agaaaaaacc gctggatgac
     4381 gtgaaagttc gccaggcact gacctacgcg gtgaacaaag acgcgatcat caaagcggtt
     4441 tatcagggcg cgggtgtatc agcgaaaaac ctgatcccgc caaccatgtg gggctataac
     4501 gacgacgttc aggattacac ctacgatcct gaaaaagcga aagccttgct gaaagaagcg
     4561 ggtctggaaa aaggtttctc catcgacctg tgggcgatgc cggtacaacg tccgtataac
     4621 ccgaacgctc gctgcatggc ggagatgatt caggcagact gggcgaaagt gggcgtgcag
     4681 gccaaaatcg tcacctacga atggggtgag tacctcaagc gtgcgaaaga tggcgaacat
     4741 cagacggtaa tgatgggctg gactggcgat aacggggacc cggataactt cttcgccacc
     4801 ctgttcagct gcgccgcctc tgaacaaggt tctaactact caaaatggtg ctacaaaccg
     4861 tttgaagatc tgattcaacc ggcgcgtgct accgacgacc ataacaaacg cgttgaactg
     4921 tacaagcaat cgcaggtggt gatgcacgat caggctccgg cactgatcat cgctcactcc
     4981 accgtgtttg aaccggtacg caaagaagtc aaaggctatg tggttgatcc attaggcaaa
     5041 catcacttcg aaaatgtctc tatcgaataa ttaaaagcca tacaagactg atggtaaagg
     5101 caaaactgcc tgatgtgctc cgcttatcag gcctacgaaa attctgcaat gtattgaatt
     5161 tgcacgattt tgtaggccgg ataaggcgtt aacgccgcat ccggcataaa caaagcgcac
     5221 tttgtcaaca atctgtatac ccggtggcgc tgtgcctctg cgtgcagcgc catccggcag
     5281 caatacttca ttctctgtcc gacaagggcg ggaattgatt tgtgagcaat acagacacgc
     5341 agttccaggc tgcgggtcac tatagagaat ccgggttatg ttgcagttta ttctccgacg
     5401 tttgggactc gtcatcccca cgtttatcgg tattaccctt ctcacatttg cctttgtcca
     5461 catgatcccg ggcgatccgg tgatgatcat ggcaggcgaa cgtgggatct cccctgagcg
     5521 tcacgcgcag ctgctggctg aactcggctt agataaaccg atgtggcagc agtatctcca
     5581 ttacatttgg ggcgtaatgc acggcgatct aggcatttca atgaaaagcc gaattccagt
     5641 atgggaagag ttcgtgccgc gctttcaggc cacgctggaa cttggcgtct gcgcgatgat
     5701 ttttgctacg gcagtcggta ttccggttgg tgtgctggct gcggttaaac gcggttccat
     5761 tttcgatcac actgcagttg gcctggcgct gaccggctac tcgatgccga tcttctggtg
     5821 gggcatgatg ctgatcatgc tggtttcggt gcactggaac ctgacgcccg cctccggtcg
     5881 cgtgagcgat atggtgttcc tcgatgactc caatccgtta accggtttta tgctaatcga
     5941 caccgccatc tggggtgaag acggcaactt tatcgatgcc gtcgcccata tgatcttgcc
     6001 tgccattgtg ctgggtacta ttccgctggc ggtcattgtg cgtatgacac gctcctcgat
     6061 gctggaagtg ctgggcgagg attacatccg caccgcgcgc gccaaagggc taacccgcat
     6121 gcgggtgatt atcgtccatg cgctgcgtaa cgcgatgctg ccggtggtga ccgttatcgg
     6181 cctgcaggtg ggaacattgc tggcgggggc gattctgacc gaaaccatct tctcgtggcc
     6241 cggtctggga cgctggttga ttgacgcact gcaacgccgc gactatccgg tagtgcaggg
     6301 cggcgtattg ctggtggcga cgatgattat cctcgtcaac ttgctggtcg atctgctgta
     6361 cggcgtggtg aacccgcgta ttcgtcataa gaagtaaggg gacatcatgt cacaggttac
     6421 tgaaaataaa gtgattagcg caccggtgcc gatgaccccg ttacaggagt tctggcacta
     6481 ttttaaacgc aacaaaggcg cggtcgtcgg gctggtttac gtcgtcatcg tgctgttcat
     6541 cgcgatcttt gccaactgga ttgcacccta taacccggcg gaacagttcc gcgatgcact
     6601 gctcgccccg ccagcctggc aggaaggcgg cagcatggcg cacttgctgg gcaccgatga
     6661 cgtaggccgt gatatgctgt cgcgcctgat gtacggtgcg cgcctgtcgc tgctggttgg
     6721 ctgtctggta gttgtgttat cgctgattat gggcgttatt ctcggcctga tcgccggtta
     6781 ctttggcggc ctagtcgata acatcattat gcgcgtggtc gatatcatgc tggcgctgcc
     6841 aagtctgctg ctggcgctgg tgctggtggc aattttcggc ccgtcgattg gtaacgccgc
     6901 gctggcactg accttcgttg ccttgccgca ctatgtgcgc ttaacccgcg ccgccgtgct
     6961 ggtggaagtt aaccgcgatt acgtcaccgc gtctcgcgtg gcgggtgccg gggcgatgcg
     7021 tcagatgttt attaacatct tcccgaactg ccttgcgccg ctgattgttc aggcgtcgct
     7081 cggtttctct aacgccattc tcgatatggc tgctcttggt ttcctcggca tgggggcaca
     7141 gccgccaacg ccggagtggg gcaccatgct ctccgacgtg ttgcagttcg cgcaaagcgc
     7201 ctggtgggtc gtgaccttcc cgggtctggc gatcctgctg acggtgctgg catttaacct
     7261 gatgggtgac ggtctgcgtg acgcgctcga tcccaaactg aagcagtaag aggttcgaga
     7321 tggcgttatt aaatgtagat aaattatcgg tgcatttcgg cgacgaaagc gcaccgttcc
     7381 gcgccgtaga ccgcatcagc tacagcgtaa aacagggcga agtggttggg attgtgggtg
     7441 agtccggctc cggtaagtcg gtcagttccc tggcgattat ggggctgatt gattatccgg
     7501 gccgcgtaat ggcggaaaaa ctggtgttta acggtcagga tttgcagcgt atatcagaaa
     7561 aagagcgccg caacctggtg ggtgccgaag tggcgatgat cttccaggac ccgatgacca
     7621 gccttaaccc gtgctacacc gtgggtttcc agatcatgga agcgattaag gtgcatcagg
     7681 gcggcaacaa aagtacccgc cgtcagcggg cgattgacct gctgaatcag gtcggtattc
     7741 ccgatccggc ttcgcgtctg gatgtttacc cgcatcagct ttccggcggc atgagccagc
     7801 gtgtgatgat cgccatggcg attgcctgtc gaccaaaact gctgattgcc gatgagccga
     7861 ccaccgcgct ggacgtgacc attcaggcgc aaatcatcga actactgctg gagctacagc
     7921 agaaagagaa catggcgctg gtgttgatta cccatgacct ggcgctggtg gcggaagctg
     7981 cgcataaaat cattgtgatg tatgcaggcc aggtggtgga aacgggcgat gcgcacgcca
     8041 tcttccatgc gccgcgtcac ccgtatactc aggcattgct gcgtgcgctg ccagaatttg
     8101 ctcaggacaa agaacgtctg gcgtcgttgc caggtgtcgt tcccggcaag tacgaccgcc
     8161 cgaacggctg cctgcttaac ccgcgctgcc cctatgccac tgacagatgt cgcgctgaag
     8221 aaccggcgct gaatatgctc gctgacgggc gtcagtccaa atgccattac ccacttgatg
     8281 atgccgggag gccgacacta tgagtacgca agaggccacc ctgcaacaac cgctgttgca
     8341 ggctatcgac ctgaaaaaac attatccggt gaagaaaggc atgttcgcgc cggaacgtct
     8401 ggttaaagcg ctggatggcg tttcgtttaa ccttgaacgt ggcaaaacgc tggcagtagt
     8461 gggcgaatct ggctgcggta aatcgaccct cggtcggttg ctgacgatga ttgaaacgcc
     8521 caccggtggc gagctgtatt accaggggca ggatctgctt aagcacgatc cgcaggcgca
     8581 gaagctgcgt cggcagaaaa tccagatcgt cttccagaac ccttacggtt cgctgaatcc
     8641 gcgtaaaaaa gtcgggcaaa ttcttgaaga gccgctgctg atcaacacca gcttaagcaa
     8701 agaacagcgt cgggaaaaag ccctgtcgat gatggcgaaa gtcggcctga aaaccgagca
     8761 ctatgaccgc tatccgcata tgttctccgg cggtcagcgt cagcgtatcg ccatcgcccg
     8821 tggtctgatg ctcgacccgg atgtggtgat tgccgatgaa ccggtttccg cgctggatgt
     8881 ttcagtgcgc gcgcaggtgc tgaatctgat gatggatttg cagcaggagt tggggctgtc
     8941 ttatgtcttt atctcccacg acctgtcggt ggtggagcac attgctgatg aagtgatggt
     9001 gatgtacctg ggccgctgcg tggagaaggg aacgaaagac caaatcttca ataacccgcg
     9061 ccatccgtac actcaggcgc tactttccgc gacgccgcgc ctgaacccgg acgatcgccg
     9121 cgagcgcatc aagctcagcg gtgaactacc aagcccactg aatccaccgc cgggttgcgc
     9181 cttcaacgcc cgctgtcgtc ggcgcttcgg cccctgcacc cagttgcagc cgcagctaaa
     9241 agactacggc ggtcaactgg tagcttgttt tgctgttgat caggatgaaa atccgcagcg
     9301 ttaactcaca aaaccggggc catgcgctcc ggtttactca atcagcttca acagtggcga
     9361 caagcatagc aaaatgccgt aaagcagaat tagccaggct ttaaatccgc gaagtttttc
     9421 cagttgtgcg actttataga tcaggaaaaa cggaatgagg cacgacacaa tgccatataa
     9481 cgggcttccc aactgaaaga acaccagcac cgatacacga aacgaaaccc aaatcgtcaa
     9541 cgtaatgacg ataaaagcgc agatcgccag agtcagcatg cgtgagttaa ttttcttggt
     9601 atcaataatt cggcttaaca gattgagaat aatgccttta atagcctcgt ggaaaccgag
     9661 ataaatgcca aagaatgcgg tcagtacggc aaagatattc agcaccgtag aggtgatatg
     9721 aatgatatgt ccagggatca cctgcgcggc cagtgccagt gccgagatat tttgttcaaa
     9781 ggcagaaacg gcttcttcgt ggctaattga gaaggtaaac gaaaaggcaa aaaacaggat
     9841 caccgcgatg agcgtgatat aactaattcg gtgggtacgc agcgcgagcc gggttgccag
     9901 tactttatct gcttcccgtt tacgataggc aatattcatt gggttaagta cctgaataaa
     9961 taccgcagaa aagaagcaaa atggaattgt aagcaggaca tcgcggaaaa agacggaggc
    10021 ttgcgggaag gcggttatat tggcgaaatt ccagtgcggg atcatcgcaa aaccgaacac
    10081 gacaataatc cctactttga ccaccaccat tggcccggaa atcttaaaca gtaatcgttc
    10141 accaccagac gcaatcgcta ccagtacggc gaaaatagcg actttataaa gtagagattg
    10201 tgaaagatcg gcatcggtta aaccgaaggt tttcaggtac gaggcgctgt cgaaaaccac
    10261 ggagagagag tagataaaaa tcccgtggat aatcatcaaa aagtagataa ccccgaggaa
    10321 aattccccag ttcttcccca ggtaatgact gataatatcg gtgtagtcat tacaggaatc
    10381 gctttcagaa agggttttta aataaatgtc ctgcactacc caggtggcag gataagcaat
    10441 gatcgctgcg gtaataaata cccaaattcc cttcaagcca atttgtactg gcatcagcac
    10501 ggttccggca ccaatcgcca tgcctatgca taataaaacc cagccgaagt cgtagcgtgt
    10561 aaacggcaat ttctgattgt gtttcgatag tgtgttgtgc tgcataaagt cctgtccaga
    10621 aagaagtcgg cctcgcaaaa atgaataaac ccagtttctc ttgcgcaaca ggtggagaag
    10681 tcgaatacgc agtatgtaaa aaatgaggca acagattcac tggggaggtc gcgaggaggc
    10741 gcaattctac aaaattgcgc atttcactac cagtctggag tcagcccgga tatgaactgc
    10801 aactattgca aataaagaaa ttaagctgga aggcatcgcg caatgaggaa gattgccggg
    10861 tgctgacagt aaaaagcaat cgtaaaatca gcggcaagcc gaaagggttc cggtgaggcg
    10921 caatgttgcg ggggctttat ccctggtggc attggttgct ggagagagaa aacccccgca
    10981 cgttgcaggt atgcacctga caacatcacg ggggctaatc ttgaacccaa acaactcaag
    11041 aatagccgcg aaacgttggc attacaacac aggcggctat atgacgctcg cagaactggg
    11101 catggccttc tggcatgatt tagcggctcc ggtcattgct ggcattcttg ccagtatgat
    11161 cgtgaactgg ctgaataagc ggaagtaacg tgtcatgcgg gcgtcaggct gccgtaatgg
    11221 caatttgcgc ccggatcagg ccgcagaggt taaactctgc ggcctttttc gtgcttactg
    11281 cgggtaaggc acccagtcgc cgccgttcag gcgaacgtac ggtttatcct ggtattgaat
    11341 aactactgca tttgagttct cggagaccgg tgctgtttgt ggcaacccac tggtgagttt
    11401 tttccagtca acattgtctt cggtgaaaat cttgccatca agaacgcgaa ccaccagatc
    11461 ggagatagcc aggaagctgc tcggttggtc gatgacaatc ggtgccccct gatgtggtgc
    11521 cttcatgccg aagaatttca ccccaacggg gacgtctgtg atagacgggc tagggatatc
    11581 acgtaggcca gatacctgca ttctgtcgcc cttcagcgcg ccgccgtgtt ccggcaccac
    11641 gaccaccatc actttacgac ccgatttctc cagttcagta aagaaggcgt ccagttcatc
    11701 aaagaatttc tgcgcccgcg ctttgtaatc cgctgttttg ctgacccccg gataatggtt
    11761 gccgtcatgc agtggaagcg tgttgtagaa cgtggcacta cggctgtttt tatctttttc
    11821 ggtaacgtcc agccagcggt taagcacggt ggtatcgtca taaaccggcg aaccatcaaa
    11881 gcccagcaaa ataaccggca gatttgtttg atccatcaat tcagtctgca tgccgccatt
    11941 ttcgcgaact tctttcaaaa aaccgccgaa ctggccgtta tgtcccatca tcaggtgctg
    12001 ggtaaagccc agtttcgaaa ggttatcaaa cagatagcag tcgttatttg ccggttgata
    12061 cagattagtg tgcgaagtct gcccgcagct ggcgcgcagt aaacggatcg ccgccgggcc
    12121 actgtaggag gtggcggagt taaagttctt gaactcaata tcgaaatgcg accacagtgg
    12181 atgcgacatc aacccggcgg cttctatatc cgaccaggaa agcgaacaga tgttaatcac
    12241 cagtagttca aatggctgag catcagcggg cagcgaagac gggaaggtcg atttacgttt
    12301 cgcctccgcg ttatagaaat tattcagcca ggcgttaagg ttcgccgttg ttggcggtgt
    12361 agtttgtgcg ggcatatcac ccactaccgg tgcgccaccc gtcgtctcaa cggttgccgc
    12421 tgcgttacca cccgtcgttg ttacagtggt cgtcggttgt ccggctggcc acaaggagaa
    12481 acttggtccc gccagggtaa gtacgttcag ccatagcagt atggcaacca caaaaacggt
    12541 aatgcgaatc cattgtgaca ggaataacca ggccactaat aaaacaaaaa tggccccaat
    12601 catctgccag ttaataaagc gtgtgacaag gtcgattaaa taatcggtac tgaaccccgc
    12661 cacctgcgaa ccctggctca ttatgctttc cgggccaggc aaccaggtgt catgccagaa
    12721 caaagcaaag ccgatcggca gggcaatcca gtggcgcaag cgatgcaggc tgtagcgcgg
    12781 aatgggcatc agcagaaacg cggcaaacac caaattgagg agcggatgga agttaagata
    12841 tcccgcccac aacaggccga acttaaccag aaaatagaag ttccagccgg aaaggccgcg
    12901 ccagtattgc cagagggaag aaggcatggc ggtattttgc gtaaattgag tcattttttg
    12961 gttgccctgg ctttttccgt gcggcgtaat gtcccggccg gtttaacata acgaggttta
    13021 gcaaagaaca aacgtaaggt gtcgcgaatg cgtcgcaaag agtgccgcgc cagatagccc
    13081 agcgggaaaa atatcagtgc gcaaacgaca ataatttcaa tgatatcgct gatggtcatc
    13141 atgatgagcg ctccacagca tcatctaaca gtcgcatggg ttctggtatt cgtcgccagt
    13201 ggcgaccatc gtgctcggca ttgatgaccg gtttagaact ttgcgttaaa ggcagcggca
    13261 tgccccattg ttctggggca agcaggcgca tctgcaccag ctcggcactg atttgatcat
    13321 cttcaaacca gaccatacgg tttgagaaaa tgtcgccagt aggcaatggg aaaatatgat
    13381 tcaacgcggt atccagatcg ttaatccgac agaatgagag aaacagcacc agccgattac
    13441 cgccaatggt catgatatcg ccggtacggt tagggcgaca cagcgtcagg gcttgttcaa
    13501 cgcggatacc cggtaccgga cgtagggcaa ccagaacgcc tttaccgtgg gcaggtaata
    13561 gagggttatt catcatgttg ttgacggcat tacagaacac atcccacttc tggaaaccac
    13621 gcagtttgag cggctgggtc attgacagca aggtagtgat atcttccggc acatagcgac
    13681 taaacttctg cccttgcacg ctttcgatca tcgtcagaca acgggagagt ggcgcattcc
    13741 acggaataac catatttgca ccgcaggcca ataacaaacg ttcatcggtg gcgcgcaggc
    13801 tagcggtatt ttcccgcacg aggattttca tcgcactacc gcgctggcga cgcagggtat
    13861 gaatgctgcg ggccagtggc tcgatttgcg cattttgctg taaagaaaag accaccgtcg
    13921 ccgcctgagc ggtacgggct tcattgaaca ggacttcgtt attgttgaac agttgccagt
    13981 gttccgatag cggcggcgca ccttccagta cagcaacatt actcagaatg cgtttttcgt
    14041 cgctgcgtgg ttggatctcc gcctcttcgc tttgaactaa tgtccagata ccattttgtt
    14101 gctgaacgct aagctgctga cgggcgctga cccctttttc gttgcaccag aaggcaatat
    14161 ccagcaaatg ttggtcaccc tgaaaacgca aactggcaag accaaaaagt gaacggtatt
    14221 cctcaagcaa caatgaaaat tgtttatcgt tattatttcc gggattaatt accaaaagcg
    14281 aacaatgggt taacctgctc catttattca ttttatccaa ccatgagcga agccgctcgg
    14341 caggaatgtt ttgccatgcg ttatttgcgc aaacaagaat aaaaaggtaa ttatggggat
    14401 caatagaaca ctgcaaatca cgggtcaaat agtatagacc tttttcatga tttagcattg
    14461 aaaataattt tattttttcc ggcccttgat aatcatctaa ttgaaagatt ttcgccggat
    14521 cgctgtccat gctaatgacc gcgacgtgtg cggtttcagc ctgggatgca attgtttgat
    14581 tcgccagact gatagcatct tcatggcgat cgacgttaaa ccaccagacg ccgcctgctg
    14641 gcatatgtcg cagctcatcc cataatgatg agataccgat agagaataca gggcccacaa
    14701 tgtccctcat gtcaccattt tgtctgatta tcagtttact agcgaaagct aagaaataaa
    14761 cctaccattg aaattaaaac ttatcagatt tagcatgtga atcaatttag aaggtcacaa
    14821 gacgtgtgat tttagtcttt tcgtttttaa ctttttctgt tgcgtaacgg aaagtcaaaa
    14881 agtgagcaaa ttcccgtctc gccgtaaaca ataaaccggc gaaatgctca cggttagaat
    14941 tgtctcatga acggtacggt tatttcatag ggatcaagca aaatgaataa caatgaacca
    15001 gatactctgc ctgatcccgc gataggctat atcttccaga atgatattgt ggcgttaaag
    15061 caggcatttt cactgcctga tattgattat gccgatattt cccaacgcga acagttggcc
    15121 gcggcattaa aacgctggcc gttgctggca gagtttgcgc aacaaaagta ggggattggt
    15181 gaatggccgt actgggattg cagggggtgc ggggaggcgt ggggacaaca accatcaccg
    15241 ccgcattagc ctggtcatta caaatgttgg gagaaaatgt cctggtggtc gatgcctgcc
    15301 cggacaactt gttgcgcctg tcatttaatg ttgattttac ccaccgtcag ggctgggcca
    15361 gagcgatgct ggatggccag gactggcgtg acgctgggtt gcgctacacc tcgcagctcg
    15421 atttgctgcc ttttggtcag ttatccattg aagaacaaga aaatccacag cacaggcaaa
    15481 cccggctgag cgatatttgc tccggcttac agcaactaaa agccagcggg cgttaccagt
    15541 ggattttaat cgacttaccg cgtgatgcct cgcagataac ccaccagctg ctgagtttgt
    15601 gcgatcactc gctggcaatc gtcaatgtgg atgccaactg ccatatccga ctgcatcagc
    15661 aagcgctgcc ggatggcgca catattttga ttaatgactt ccgtattggc agtcaggttc
    15721 aggacgatat ttaccagctt tggttgcaaa gccagcgccg attactgccg atgctcattc
    15781 atcgtgatga agcgatggct gaatgcctgg cggctaagca accagtaggt gaatatcgca
    15841 gtgatgcgct ggcggctgaa gagatactga cgctggcgaa ctggtgcctg ttgaactact
    15901 ccgggctgaa aacgccagtc gggagtgcat catgagtatc ctgacccggt ggttgcttat
    15961 cccgccggtc aacgcgcggc ttatcggggg ttatcgcgat tatcgtcgtc acggtgcgtc
    16021 ggctttcagc gcgacgctcg gctgtttctg gatgatcctg gcctggattt ttattccgct
    16081 ggagcacccg cgctggcagc gtattcgcgc agaacataaa aacctgtatc cgcatatcaa
    16141 cgcctcgcgt ccgcgtccgc tggacccggt ccgttatctc attcaaacat gctggttact
    16201 gatcggtaca tcgcgcaaag aaacgccgaa accgcgcagg cgggcatttt caggtctgca
    16261 gaatattcgt ggacgttacc atcaatggat gaacgagctg cctgagcgcg ttagccataa
    16321 aacacagcat cttgatgaga aaaaagagct cggtcatttg agtgccgggg cgcggcggtt
    16381 gatcctcggt atcatcgtca ccttctcgct gattctggcg ttaatctgcg ttactcaacc
    16441 gtttaacccg ctggcgcagt ttatcttcct gatgctgctg tggggtgtag cgctgatcgt
    16501 acggcggatg ccggggcgct tctcggcgct aatgttgatt gtgctgtcgc tgaccgtttc
    16561 ttgccgttat atctggtggc gttacacctc tacgctgaac tgggacgatc cggtcagcct
    16621 ggtgtgcggg cttattctgc tcttcgctga aacgtacgcg tggattgtgc tggtgctcgg
    16681 ctacttccag gtcgtatggc cgctgaatcg tcagccggtg ccattgccga aagatatgtc
    16741 gctgtggccg tcggtggata tctttgtccc gacttacaac gaagatctca acgtggtgaa
    16801 aaataccatt tacgcctcgc tgggtatcga ctggccgaaa gacaaactga acatctggat
    16861 cctcgatgac ggcggcaggg aagagtttcg ccagtttgcg caaaacgtgg gggtgaaata
    16921 tatcgcccgc accactcatg aacatgcgaa agcgggcaac atcaacaatg cgctgaaata
    16981 tgccaaaggc gagttcgtat cgattttcga ctgcgaccac gtaccaacgc gatcgttcct
    17041 gcaaatgacc atgggctggt tcctgaaaga gaaacagctg gcgatgatgc agacgccgca
    17101 ccacttcttc tcgccggacc cgtttgaacg caacctgggg cgttttcgta aaacgccgaa
    17161 cgaaggcacg ctgttctatg gtctggtgca ggatggcaac gatatgtggg acgccacttt
    17221 cttctgcggt tcctgtgcag taattcgtcg caaaccattg gatgaaattg gcggtattgc
    17281 tgtcgaaacg gtaaccgaag atgcgcacac gtctctgcgc ctgcaccgtc gtggctacac
    17341 ctctgcatat atgcgtattc cgcaggcggc ggggctggcg accgaaagtc tgtcggcgca
    17401 tatcggtcag cgtattcgct gggcacgcgg gatggtgcaa atcttccgtc tcgataaccc
    17461 gcttaccggt aaagggctga aattcgccca gcggctgtgc tacgtcaacg ccatgttcca
    17521 cttcttgtcg ggcattccac gactgatctt cctgactgcg ccgctggcgt tcctgctgct
    17581 tcatgcctac atcatctatg cgccagcgtt gatgatcgcc ctgttcgtgc tgccgcatat
    17641 gatccatgcc agcctgacca actcgaagat ccagggcaaa tatcgccact ctttctggag
    17701 tgaaatctac gaaacggtgc tggcgtggta tatcgcacca ccgacgctgg tggcgctgat
    17761 taacccgcac aaaggcaaat ttaacgtcac cgccaaaggt ggactggtgg aagaagagta
    17821 cgtcgactgg gtgatctcgc ggccctacat cttccttgtt ctgctcaacc tggtgggtgt
    17881 tgcggtcggc atctggcgct acttctatgg cccgccaacc gagatgctca ccgtggtcgt
    17941 cagtatggtg tgggtgttct acaacctgat tgttcttggc ggcgcagttg cggtatcggt
    18001 agaaagcaaa caggtacgcc gatcgcaccg cgtggagatg acgatgcccg cggcaattgc
    18061 ccgcgaagat ggtcacctct tttcgtgtac cgttcaggat ttctccgacg gtggtttggg
    18121 gatcaagatc aacggtcagg cgcagattct ggaagggcag aaagtgaatc tgttgcttaa
    18181 acgcggtcag caggaatacg tcttcccgac ccaggtggcg cgcgtgatgg gtaatgaagt
    18241 tgggctgaaa ttaatgccgc tcaccaccca gcaacatatc gattttgtgc agtgtacgtt
    18301 tgcccgtgcg gatacatggg cgctctggca ggacagctac ccggaagata agccgctgga
    18361 aagtctgctg gatattctga agctcggctt ccgtggctac cgccatctgg cggagtttgc
    18421 gccttcttcg gtgaagggca tattccgtgt gctgacttct ctggtttcct gggttgtatc
    18481 gtttattctg cgccgcccgg agcggagcga aacggcacaa ccatcggatc aggctttggc
    18541 tcaacaatga tgataacgcg atgaaaagaa aactattctg gatttgtgca gtggctatgg
    18601 ggatgagtgc gttcccctct ttcatgacgc aggcgacgcc agcaacgcaa ccactgatca
    18661 atgctgagcc agctgtagcc gcccagacgg aacaaaatcc gcaggtgggg caagtgatgc
    18721 cgggcgtgca gggcgctgat gcgccagtcg tggcgcagaa cggtccttcg cgtgatgtga
    18781 agctgacctt tgcgcaaatt gcgcctccgc cgggcagcat ggtgctacgt ggcattaacc
    18841 cgaacggcag cattgagttt ggtatgcgca gcgatgaagt ggtgacgaag gcgatgctca
    18901 acctcgaata catgccatcg ccatcgttac tgcctgtcca gtcgcagtta aggtttatct
    18961 caatgatgag ctgatgggcg tactgccagt gaccaaagaa cagttgggta aaaaaacgct
    19021 ggcgcaaatg cccattaacc cactgtttat taccgacttc aaccgtgtgc ggctggagtt
    19081 tgtcggccat tatcaggacg tgtgcgaaaa cccggccaga accacgcttt ggctggatgt
    19141 tgggcggagc agtggactgg atctgaccta tcagaccctg aatgtgaaga atgacctgtc
    19201 acacttcccg gtgccattct ttgacccgcg cgataaccgt actaacacct tgccgatggt
    19261 ctttgcgggt gcgccggatg ttgggctgca acaagcatct gccattgtcg cctcgtggtt
    19321 tggttcgcgt tctggctggc gtgggcagaa cttcccggtg ctctataacc aactgccgga
    19381 tcgtaatgcc attgtctttg caaccaacga caaacggccg gacttcctgc gcgatcatcc
    19441 ggcggtaaaa gccccggtga ttgagatgat caaccatccg cagaatcctt acgtcaaact
    19501 gctggtggtg tttggtcgtg acgacaaaga cctgttgcag gcagcgaaag gtatcgctca
    19561 gggtaacatt ctgttccgtg gtgaaagcgt ggtagtgaat gaagtgaaac cgctgctacc
    19621 gcgtaagccg tacgatgcgc cgaactgggt acgtaccgat cgtccggtca catttggcga
    19681 actgaaaacc tatgaagaac agttacaatc cagcggtctt gagccagcag cgattaacgt
    19741 ttcgctaaac ctgccgccgg atctctacct gatgcgcagt accggcattg atatggatat
    19801 taattaccgc tacaccatgc cgccggtgaa agacagttcg cggatggata tcagcctgaa
    19861 taaccagttc ctgcaatcct tcaacctgag cagcaaacag gaggcgaacc gcctgctgct
    19921 gcggattccg gtattacaag gtttgctgga tggcaaaaca gatgtctcta ttccggcgct
    19981 gaaactgggc gcgaccaacc agctgcgctt cgactttgag tatatgaacc cgatgccggg
    20041 cggttcggtg gataactgta ttaccttcca gccggtgcag aatcatgtgg tgattggtga
    20101 cgactccacc atcgacttct cgaagtatta ccacttcatc ccgatgccgg atctacgcgc
    20161 ctttgctaac gcgggcttcc cattcagccg gatggcggat ctgtcgcaaa ccatcaccgt
    20221 gatgccgaaa gcgcctaacg aagcacagat ggaaacgttg ctgaatactg ttggttttat
    20281 cggcgcacag acgggcttcc cggcgattaa tctgacggtg accgatgatg gcagcaccat
    20341 tcagggcaaa gatgccgaca tcatgatcat cggtggtatc ccggacaaac tgaaagacga
    20401 taagcagatc gacctattgg tgcaggcgac cgaaagctgg gtgaaaacac cgatgcgcca
    20461 gaccccgttc cccggcattg tgccggacga gagcgatcgc gcggcagaaa cccggtcaac
    20521 gctgacctct tccggtgcga tggcggcggt gattggcttc cagtcgccgt ataacgacca
    20581 gcgcagcgtg attgcgctgt tggcagatag cccacgcggt tatgaaatgc ttaacgatgc
    20641 ggtgaacgat agcggcaaac gcgccaccat gttcggttcg gtcgcggtga tccgcgagtc
    20701 cggtatcaac agcctacgtg ttggcgacgt ttattacgta ggtcatctgc cgtggttcga
    20761 gcgcgtgtgg tatgcgctgg caaaccatcc gattctgctg gcggtgctgg cggctatcag
    20821 tgtgatattg ctggcatggg tactgtggcg tctgctgcga attattagtc gtcgtcgtct
    20881 taacccggat aacgagtaat tgaagatgaa tgtgttgcgt agtggaatcg tgacgatgct
    20941 gttgttggct gcctttagtg ttcaggcagc ctgtacctgg cctgcctggg agcagtttaa
    21001 aaaggattac atcagtcagg aagggcgcgt catcgacccc agcgacgcgc gcaaaatcac
    21061 cacctccgaa gggcaaagtt acggtatgtt ctttgccctg gcggctaacg accgtgcagc
    21121 tttcgataat attctcgact ggacgcagaa caatctcgct cagggttctt taaaagaacg
    21181 tttgcccgcc tggctgtggg gcaagaaaga gaacagtaag tgggaagtgc tggacagcaa
    21241 ttcggcctcc gatggtgatg tctggatggc ctggtcgttg ctggaggcgg ggcgtttgtg
    21301 gaaagagcag cgttataccg acatcggcag cgcgttgcta aaacgtatcg cgcgggagga
    21361 agtggtgacg gtgcctgggc tgggttccat gttgttaccg ggcaaagtgg gttttgctga
    21421 ggataacagc tggcgtttta accccagcta cctgccgccg acgctggcgc agtatttcac
    21481 ccgctttggc gcgccgtgga ctacgctgcg cgaaaccaat caacgtttat tgctggaaac
    21541 cgccccgaaa ggcttttcgc cagactgggt gcgttatgag aaagacaaag gctggcagct
    21601 aaaagccgaa aaaacattga tcagcagcta cgacgccatc cgcgtttaca tgtgggtagg
    21661 catgatgcct gacagcgatc cgcagaaagc gcggatgctc aaccggttta aaccgatggc
    21721 gacattcact gagaaaaacg gttatccgcc ggaaaaagtg gatgtggcta cggggaaagc
    21781 gcagggtaaa ggaccagtcg gtttttctgc cgccatgctg ccctttttac aaaaccgcga
    21841 tgcgcaggcc gttcagcgtc agcgcgtggc cgataacttt cccggcagcg atgcctatta
    21901 caactatgtg ctgaccctgt ttggacaagg ctgggatcaa caccgtttcc gcttctcgac
    21961 aaaaggtgag ttattacctg actggggcca ggaatgcgca aattcacact aaacatattc
    22021 acgctttccc tcggtctggc cgtcatgccg atggtcgagg cagcaccaac cgctcagcaa
    22081 cagttgctgg agcaagttcg gttaggcgaa gcgacccatc gtgaagatct ggtgcaacag
    22141 tcgttatatc ggctggaact tattgatccg aataacccgg acgtcgttgc cgcccgtttc
    22201 cgttctttgt tacgttaggg cgatattgat ggcgcgcaaa aacagctcga tcggctgtcg
    22261 cagttagcgc cgagttcaaa tgcgtataaa tcgtcgcgga ctacgatgct actttccacg
    22321 ccggatggtc gtcaggcact gcaacaggca cgattgcagg cgacgaccgg tcatgcagaa
    22381 gaagctgtgg cgagttacaa caaactgttc aacggtgcgc cgccggaagg tgacattgct
    22441 gtcgagtact ggagtacggt ggcgaaaatt ccggctcgcc gtggcgaagc gattaatcag
    22501 ttaaaacgca tcaatgcgga tgcaccgggc aatacgggcc tgcaaaacaa tctggcgcta
    22561 ttgctgttta gtagcgatcg ccgtgacgaa ggttttgccg tcctggaaca gatggcaaaa
    22621 tcgaacgccg ggcgcgaagg ggcctctaaa atctggtacg ggcagattaa agacatgccc
    22681 gtcagtgatg ccagtgtgtc ggcgctgaaa aaatatctct cgatctttag tgatggcgat
    22741 agcgtggcgg ctgcgcaatc gcaactggca gaacagcaaa aacagctggc cgatcctgct
    22801 ttccgcgctc gtgcgcaagg tttagcggcg gtggactctg gtatggcggg taaagccatt
    22861 cccgaactac aacaggcggt gcgggcgaac ccgaaagaca gtgaagctct gggggcgctg
    22921 ggccaggcgt attctcagaa aggcgatcgc gccaatgcag tggcgaatct ggaaaaagcc
    22981 ctcgcactgg acccgcacag cagcaacaac gacaaatgga acagtctgct gaaagtaaac
    23041 cgctactggc tggcgatcca gcagggcgat gctgcgctga aagccaataa tcctgaccgg
    23101 gcagaacgcc tgttccagca ggcgcgtaat gtcgataaca ccgacagtta tgcagtgctg
    23161 gggctgggcg atgtggcgat ggcgcgaaaa gattatcccg ccgccgaacg ttattatcag
    23221 cagaccttgc gtatggacag cggcaacact aacgccgtgc gcgggctggc aaatatttac
    23281 cgccagcaat cgccagaaaa agctgaagcg tttatcgcct cgctctctgc cagtcagcgg
    23341 cgtagcattg atgatatcga acgcagcctg caaaacgacc gtctggcaca gcaggcagag
    23401 gcactggaaa accagggcaa atgggcgccg gcggcagcac ttcagcggca acgactggcg
    23461 ctggaccccg gcagcgtatg gattacttac cgactttcgc aggatctctg gcaggccgga
    23521 caacgcagcc aggccgatac gttaatgcgc aatctggcgc agcagaagtc gaacgacccg
    23581 gagcaggttt acgcttacgg gctgtacctc tctggtcatg accaggacag agcggcgctg
    23641 gcgcatatca acagcctgcc gcgagcgcag tggaacagca atattcagga gctggttaat
    23701 cgactgcaaa gcgatcaggt gctggaaacc gctaatcgcc tgcgagaaag cggcaaagaa
    23761 gctgaagcgg aagcgatgct gcgtcagcaa ccaccttcca cgcgtattga cctcacgctg
    23821 gctgactggg cgcagcagcg acgtgattac accgccgccc gcgctgcata tcagaatgtc
    23881 ctgacgctgg agccaactaa cgccgatgcc attctggggc tgacggaagt ggatattgct
    23941 gccggtgaca cagcggcggc acgtagccag ctggcgaaac tgcccgctac cgataacgcc
    24001 tcgctgaaca cacagcggcg cgtggcgctg gcgcaggcgc agcttggaga taccgcagcg
    24061 gcgcagcaga cgtttaataa gttgatcccg caggcaaaat ctcagccacc gtcgatggaa
    24121 agcgcgatgg tgctgcgcga cggtgcgaag tttgaagcgc aggcgggcga tccaaagcag
    24181 gcgctggaaa cctacaaaga cgccatggtc gcatccggtg tgactacgac gcgtccgcag
    24241 gataacgaca cattcacccg actgacccgt aacgacgaga aagatgactg gctgaaacgc
    24301 ggcgtgcgca gcgatgcggc gggcctctat cgccagcagg atcttaacgt caccctcgag
    24361 cacgattact ggggttcgag cggcaccggt ggttactccg atctgaaagc gcacactacc
    24421 atgttgcagg tggatgcgcc gtattctgac gggcggatgt tctttcgcag tgatttcgtc
    24481 aatatgaacg tcggcagttt ctccactaat gccgatggca aatgggatga caactggggc
    24541 acctgtacat tacaggactg tagtggcaac cgcagccagt cggactccgg tgccagcgtg
    24601 gaggtcggct ggcgaaatga cgtctggagc tgggatatcg gtaccacgcc gatgggcttc
    24661 aacgtggtgg atgtggtcgg cggcatcagt tacagcgatg atatcgggcc gctgggttac
    24721 accgttaacg cccaccgtcg gcccatctcc agttctttgc tggcctttgg tgggcaaaaa
    24781 gactccccga gcaataccgg gaaaaaatgg ggtggcgtac gtgccgacgg tgtggggcta
    24841 agtctgagct acgataaagg tgaagcaaac ggcgtctggg catcgcttag tggcgaccag
    24901 ttaaccggta aaaatgtcga agataactgg cgcgtgcgct ggatgacggg ctattactat
    24961 aaggtcatta accagaacaa tcgccgcgtc acaatcggcc tgaacaacat gatctggcat
    25021 tacgacaaag atctgagtgg ctactcactc ggtcagggcg gttactacag tccgcaggaa
    25081 tacctgtcgt ttgctatacc ggtgatgtgg cgggagcgca cggaaaactg gtcgtgggag
    25141 ctgggggcgt ctggctcgtg gtcgcattca cgcaccaaaa ccatgccgcg ttatccgctg
    25201 atgaatctga tcccgaccga ctggcaggaa gaagctgcgc ggcaatccaa cgatggcggc
    25261 agcagtcagg gcttcggcta cacggcgcgg gcattacttg aacgacgtgt tacttccaac
    25321 tggtttgttg gcacggcaat tgatatccag caggcgaaag attacgcacc cagccatttc
    25381 ctgctctacg tacgttattc cgccgccgga tggcagggtg acatggattt accgccgcag
    25441 ccgctgatac cttacgccga ctggtaagtt ttcagatagc gcctctctta atgccgctgc
    25501 gatcgggtat actcgggcgg caatctggga tttccggggg gagacaattt gcgcgtaagt
    25561 cgctcgttaa caatcaagca gatggcaatg gtggcagccg ttgtcctggt gttcgttttt
    25621 attttttgca ccgttttgct gttccatctg gtccagcaga atcgctataa cacggctacg
    25681 caactggaaa gcattgctcg ctctgtacgc gaacccttat cttcagctat tttgaaaggc
    25741 gatattcccg aagcggaagc tattcttgcc agcattaaac cggcaggcgt ggtcagccgt
    25801 gccgatgtag tgctgcctaa ccagttccag gcgctgcgta aaagttttat tccagagcgc
    25861 ccggtgccgg taatggttac tcgcctgttt gagctaccgg ttcaaatctc gctgggcgtt
    25921 tactcgctcg aacgtccggc aaacccgcag ccaattgcct atctggtact acaggcggat
    25981 tccttccgta tgtataagtt cgtgatgagc accctctcaa cgttagtgac catttactta
    26041 cttttgtcgc ttatcctgac cgtcgccatc agctggtgca ttaaccgcct gattttgcat
    26101 ccgttacgca atattgctcg cgaacttaac gccatcccag cccaggagct tgttggtcac
    26161 caactggcat taccgcgtct gcatcaggac gatgaaatcg gtatgttggt gcgcagttac
    26221 aacctcaacc agcaattgct gcagcgccat tatgaagaac agaacgaaaa tgcgatgcgc
    26281 ttcccggtgt cggatttgcc gaacaaagcc ttgctgatgg agatgctgga gcaggttgtc
    26341 gcgcgtaaac aaaccaccgc gctgatgatc atcacctgtg aaaccctgcg tgatactgcg
    26401 ggcgtgctga aagaggcgca acgagaaatt ctgctgctga cgctggtgga aaaactcaaa
    26461 tcggtactgt cgccacgtat gatcctcgcg cagattagcg gttatgactt tgctgtcatt
    26521 gccaacggtg tacaggaacc gtggcacgca atcaccttag gtcagcaagt gctcactatc
    26581 atgagcgagc gcctgccgat tgaacgtatt caactccgtc cgcactgtag cattggcgtg
    26641 gcgatgttct acggcgatct caccgccgaa cagctttaca gtcgcgctat ttctgcggca
    26701 tttaccgctc gccataaagg caagaatcag attcagttct ttgatccgca gcagatggaa
    26761 gccgcccaga agcggttgac ggaagagagc gatatcctta atgcactgga aaatcatcag
    26821 tttgctattt ggttacagcc acaggtcgag atgaccagcg gtaaactggt cagtgcggaa
    26881 gtgttactgc gtatccagca accggatggc agttgggacc tgccggatgg cttaatcgat
    26941 cgcattgagt gctgtgggct gatggttacc gtcggtcact gggtgctgga agagtcctgt
    27001 cgattgcttg cagcctggca agagcgcggc attatgctgc ccttgtcggt aaacctctct
    27061 gcgctgcaac tgatgcaccc aaatatggtg gcggatatgc tggaactgtt aacccgctat
    27121 cgcattcaac cgggaacact gattctggaa gtgacagaaa gccgacgtat tgacgaccct
    27181 catgctgcgg tggcaatcct ccgtccgctg cgcaatgccg gagttcgggt ggcgctggat
    27241 gatttcggca tgggctacgc agggctgcgt cagctgcagc atatgaaatc gttgccaatc
    27301 gacgtactga aaatcgacaa aatgtttgtt gaaggcttgc cggaagatag cagcatgatt
    27361 gctgcaatta tcatgctggc gcagagcctg aacttacaaa tgattgccga aggcgtggag
    27421 actgaagcac aacgcgactg gctggcaaaa gcgggcgttg gtattgccca gggcttcctt
    27481 tttgctcgcc cactccctat tgaaatcttc gaagagagtt acctggaaga aaagtagcta
    27541 ccccaaactg attacaaaac tttaaaaagt gctggtttgt gcgagccagc tcaaactttt
    27601 taaccttttt gtttcaatta tgatccaggt acatttctgt gatgttgtct gggtgttatt
    27661 ttaaggccgc aggtacctca taaccttaca agacctgtag ttttactaaa ggacacccta
    27721 tgaaaacctc tctgtttaaa agcctttact ttcaggtcct gacagcgata gccattggta
    27781 ttctccttgg ccatttctat cctgaaatag gcgagcaaat gaaaccgctt ggcgacggct
    27841 tcgttaagct cattaagatg atcatcgctc ctgtcatctt ttgtaccgtc gtaacgggca
    27901 ttgcgggcat ggaaagcatg aaggcggtcg gtcgtaccgg cgcagtcgca ctgctttact
    27961 ttgaaattgt cagtaccatc gcgctgatta ttggtcttat catcgttaac gtcgtgcagc
    28021 ctggtgccgg aatgaacgtc gatccggcaa cgcttgatgc gaaagcggta gcggtttacg
    28081 ccgatcaggc gaaagaccag ggcattgtcg ccttcattat ggatgtcatc ccggtgagcg
    28141 tcattggcgc atttgccagc ggtaacattc tgcaggtgct gctgtttgcc gtactgtttg
    28201 gttttgcgct ccaccgtctg ggcagcaaag gccaactgat ttttaacgtg atcgaaagtt
    28261 tctcgcaggt catcttcggc atcatcaata tgatcatgcg tctggcacct attggtgcgt
    28321 tcggggcaat ggcgtttacc atcggtaaat acggcgtcgg cacactggtg caactggggc
    28381 agctgattat ctgtttctat attacctgta tcctgtttgt ggtgctggta ttgggttcaa
    28441 tcgctaaagc gactggtttc agtatcttca aatttatccg ctacatccgt gaagaactgc
    28501 tgattgtact ggggacttca tcttccgagt cggcgctgcc gcgtatgctc gacaagatgg
    28561 agaaactcgg ctgccgcaaa tcggtggtgg ggctggtcat cccgacaggc tactcgttta
    28621 accttgatgg cacatcgata tacctgacaa tggcggcggt gtttatcgcc caggccacta
    28681 acagtcagat ggatatcgtc caccaaatca cgctgttaat cgtgttgctg ctttcttcta
    28741 aaggggcggc aggggtaacg ggtagtggct ttatcgtgct ggcggcgacg ctctctgcgg
    28801 tgggccattt gccggtagcg ggtctggcgc tgatcctcgg tatcgaccgc tttatgtcag
    28861 aagctcgtgc gctgactaac ctggtcggta acggcgtagc gaccattgtc gttgctaagt
    28921 gggtgaaaga actggaccac aaaaaactgg acgatgtgct gaataatcgt gcgccggatg
    28981 gcaaaacgca cgaattatcc tcttaatctc accacttatg cccgcactcc cttccccgag
    29041 tgcgggtgtt tgcgcataat tgacccctgt gcctgaactt accacctggg tgagttcagg
    29101 tttatttcac ttcttccgtg acattttcat gttcttgcgg tctaacacga agtgttttta
    29161 acgtcattta gactgactga caacgggcag ttttttatta ccaggatagt tgatcagggg
    29221 ttcacatgca gggcacaaaa attcgacttt tagcgggcgg tttgctgatg atggccactg
    29281 ctggctatgt gcaggcagat gcgctccagc ctgatccagc atggcaacag gggacgcttt
    29341 ccaacggttt acagtggcaa gtgctgacca ccccccagcg tcccagcgat cgtgttgaaa
    29401 ttcgcctgct ggttaatacc ggttcgctcg ccgaaagtac acaacagagc ggttacagtc
    29461 acgccatccc tcgtattgcg ctaacgcaaa gcggtggcct tgacgcagca caggcgcgtt
    29521 cattgtggca gcaggggatc gaccctaaac gcccgatgcc gccggtaatt gtctcttatg
    29581 acaccacgct gtttaatctg agtttgccca ataaccgtaa cgatttgctg aaagaagcgc
    29641 tctcttatct ggcaaatgcc actggcaaat tgaccatcac accagaaacc atcaaccacg
    29701 cgctgcaaag tcaggacatg gtggcaacct ggcctgccga tactaaagag ggctggtggc
    29761 gctatcgtct gaaagggtca accttgttag gtcacgatcc tgccgatccg ctgaaacaac
    29821 ccgttgaagc ggaaaaaatt aaagatttct atcagaaatg gtacaccccg gatgcaatga
    29881 cgctactggt ggtgggaaac gtggatgcgc gctcggttgt cgaccaaatc aacaaaacgt
    29941 ttggcgaact gaaaggcaaa cgtgaaacgc cagctccggt gccgacgctt tctccgctgc
    30001 gtgcggaagc ggtgagtatt atgactgacg cggtgcgtca ggaccggtta tctatcatgt
    30061 gggatacgcc gtggcagccg attcgtgaat cagccgcact gctgcgctac tggcgtgcgg
    30121 acctggcccg cgaggcgctg ttctggcatg ttcagcaagc gttaagcgcc agtaacagca
    30181 aagacatcgg tcttggattt gactgccgtg tgctgtatct gcgtgcgcag tgtgccatca
    30241 acatcgaatc accaaacgac aagctgaaca gcaaccttaa tctggtggcg cgtgaactgg
    30301 cgaaggttcg cgataaaggt ctgccggaag aagagttcaa tgcgttagtg gcgcaaaaga
    30361 aactggagct gcagaaactg tttgccgcct atgcgcgggc tgataccgat attctgatgg
    30421 gtcagcggat gcgttcgttg caaaatcagg tcgtcgatat cgcgccggag cagtatcaga
    30481 aactgcggca ggatttcctt aacagcctga cggtggagat gttaaatcag gatctgcgtc
    30541 agcagttgtc gaatgatatg gcgttaatac tgctgcagcc gaaaggcgag ccggaattta
    30601 acatgaaagc gttgcaggcg gcctgggatc aaatcatggc cccatcgact gctgctgccg
    30661 ccacctctgt cgccacggat gacgtacatc ctgaagtgac ggatattcca cctgcacagt
    30721 aatgcctgat ggtgatgagt tagcacaacc atcaggtatt tatggatgag ctggatagag
    30781 gggtaacact ttatccagcc ttttgcatat gctgcgttta cgctggcatc gcctcacgcg
    30841 ggataatcgc gccgcgatac tgaataacgg tacttgcggt caggtgcccg cgtttcgccg
    30901 cgttttccgc actgccgccc gtcaaacgta ccgccagata accggcgctg aaagagtcgc
    30961 cagctgcggt ggtatcgatc actttttctt tcggcagttt caccgccgga acatccacta
    31021 acccttcgcc agcaatggac accaggcaag aatccgcccc gcgtttcacc accacttctt
    31081 tcacgcccgc gttatgggtg cgcgcaatga cgtcttccac cggctgttga ccccacagcg
    31141 cgtcttcatc gtccagcgtc aggaaggcga tatccgtgca ttccagcatt tgttggtaca
    31201 cctgctgtgt ctcttctttg ctggcccaca ggcgcggacg atagttattg tcgaaaatca
    31261 cttttccgcc gttggcgcgg cattcgcgca gcagggaaag cagcttttcg cggctggtcg
    31321 ggcttaagat cgccaggcta atcccgctca ggtagagata atcgaaattc gccagctctt
    31381 cgcaaatcgc cgtagactgc tcactctcca gccagaattt ggcggcggct tcgttccgcc
    31441 agtagtagaa cgtacgctcg ccggtgctgt cggtttcaat gtagtaaagg cccggcagac
    31501 ggttttccat ccgttgggtc agggaaatat caacgttctc gccgtgccag gcgtccagca
    31561 tctgctggct aaaactgtcc gttcccagcg ccgttacgta atgaacggtt aatgccgcag
    31621 gatcgacctg acgggcgata tagacggaag tgttcagggt atcgccgccg aaaccgcgct
    31681 taacgtccgc gcctttctcg gaaagctcaa tcatgcattc gccaatcacg gcaatctttt
    31741 tggacatagt cgtgaacctg atctgtaaaa aattagcgtt agtttgcgct gtggtggttt
    31801 gctggtcaac aatattaaaa cagtgttcca ttattttttt gagccagaac gtattcctga
    31861 aagattccgt tgtggagtga ggaaatttac cccggccagg cggtaaagtt ctgcccttac
    31921 gcgccgataa tctttgtcga gtccgggcag catcactttt aaacacagga catctttgat
    31981 gataaggcag gttatccagc gaataagcaa ccctgaagca agcatcgaga gcttgcagga
    32041 acggcgtttt tggttgcagt gtgagcgtgc ttacacctgg cagccgatct atcaaacatg
    32101 cgggcggtta atggccgtgg agctattaac ggtggtcacg catcccttga acccttcgca
    32161 acgcctgccg ccggatcgct attttactga aatcaccgtc agccatcgga tggaggttgt
    32221 gaaagagcag attgatttgc tggcgcaaaa agccgacttc tttatagagc acggcctgct
    32281 ggcatcggtc aatattgatg gccctacgct catcgccctg cgtcagcaac caaaaatcct
    32341 gcgccaaatt gagcgtcttc cctggctgcg tttcgaactg gtggagcata tccgtctgcc
    32401 gaaagattca acctttgcct cgatgtgtga atttggcccg ctgtggctgg gtgattttgg
    32461 taccgggatg gcaaatttct ctgcactaag tgaagtgcgt tatgactaca tcaaaatcgc
    32521 gcgagaactg tttgtgatgc tgcgtcagtc gccggaagga cgcacactct tttctcagct
    32581 tttacatcta atgaatcgct attgtcgcgg ggtgattgtt gagggcgtag aaacgccgga
    32641 agagtggcgt gatgtacaga actcgcccgc attcgccgca caaggctggt ttctttcacg
    32701 cccggcaccg atagaaacgc tgaatacggc ggttctggcg ctataagctg cctcattttc
    32761 cgcctggctg gactatcttt aggactggca caggaagaat atgaggaagc gaacgatgag
    32821 caaggcaggc aaaataactg ctgcgatttc aggggctttc ttgttgttga ttgtcgtggc
    32881 gatcattttg attgcaactt ttgactggaa tcgactcaaa ccgaccatca accagaaagt
    32941 ctctgcggag ttgaatcgtc cgttcgctat ccgtggcgat ctgggcgtgg tgtgggagcg
    33001 gcaaaaacaa gaaactggct ggcgcagctg ggtgccgtgg cctcatgtac atgcagaaga
    33061 catcattctt ggcaatccac cggatattcc cgaagtcacg atggtgcatt tgccgcgagt
    33121 agaggcaacg ctggcaccgc tggcgctgct gaccaaaacg gtctggctgc cgtggatcaa
    33181 gctcgaaaag cccgacgcgc gcctgattcg cctctccgaa aagaacaata actggacgtt
    33241 taatctcgcc aacgatgata acaaagacgc gaatgcaaag ccgtcggcat ggtcgtttcg
    33301 gctggataat attcttttcg atcaagggcg gatcgccatt gatgacaaag taagcaaagc
    33361 ggatctggaa atttttgttg atccgttagg caagccgctg ccgttcagcg aagttactgg
    33421 atcgaaaggt aaagcggata aagaaaaggt gggcgattac gtttttggcc tgaaggcgca
    33481 gggacgttat aacggtgaac agctcacggg tacgggaaaa ataggcggta tgctggcgct
    33541 gcgtggcgaa gggacgccgt ttccggtaca ggctgatttc cgttcaggta atacccgtgt
    33601 tgcttttgat ggcgtcgtga atgacccaat gaagatgggc ggtgtcgatt tacggcttaa
    33661 attttctggc gattcactgg gtgatctcta tgaactgacg ggcgttctgc tgcccgatac
    33721 cccgccgttt gaaacggatg gtcggctggt agcgaaaatc gacactgaaa aatcgtcggt
    33781 ctttgattat cgcggtttta atgggcgaat tggcgatagc gatatccacg gttctctggt
    33841 ctacaccacc ggaaagccgc gaccaaaact ggaaggtgat gtcgagtcgc ggcaattgcg
    33901 gctggcggac ctgggaccgt tgattggcgt tgattcgggg aaaggggcag aaaagtcgaa
    33961 acggtctgaa cagaagaagg gcgaaaaaag cgttcagcct gcgggcaaag tgctgcctta
    34021 tgaccgcttc gaaaccgata aatgggacgt tatggatgcc gatgttcgct tcaaaggccg
    34081 acgtatcgag catggcagta gcctgccgat tagcgatctt tctactcata tcatcctcaa
    34141 aaatgctgac ctgcgcctgc aaccgctgaa atttggcatg gcgggcggca gcattgcggc
    34201 gaatatccat ctggaaggcg ataaaaagcc gatgcagggg cgggcagata ttcaggctcg
    34261 tcgactgaaa ctgaaagaac tgatgcccga tgtggaactg atgcagaaga cgctggggga
    34321 aatgaacggt gacgcggaac tacgcggtag cggtaactcg gtggcggcgc ttttaggcaa
    34381 cagtaacggc aacctgaaac tgttgatgaa tgacgggctg gtgagccgca acctgatgga
    34441 gattgttggg ctgaatgtcg gcaactacat tgtcggtgcg atatttggtg acgatgaggt
    34501 gcgggtgaac tgcgcggcgg cgaatctgaa tattgccaac ggcgtagcgc gcccgcagat
    34561 ttttgctttc gatactgaga acgcgttgat taatgttacc ggcacggcaa gttttgcttc
    34621 ggaacagctg gatttgacta ttgatccgga gagtaaaggg attcggatta tcacactgcg
    34681 ttcgccgctg tatgtgcgtg ggacgtttaa aaatccgcag gctggggtga aagccggacc
    34741 gctgattgcc cgtggtgccg ttgcggcggc actggcaacg ctggtaacac cggcggcggc
    34801 gttattggca ctgatctcac cttccgaagg ggaggctaat cagtgtcgga cgatattgtc
    34861 gcagatgaag aagtgatgag gttggattgt gtttgctggt gagttcggga gcagcgtgtt
    34921 cccctcaccc taatcctctc cccaaagggg cgaggagact gttttgtgcg tgttgaaaca
    34981 acgagtaccg cttttccgtt cactttctgg gggagggctg ttatgttcgc gatataacat
    35041 ctcgctccgc ttttctccct ctccctttca gggagagggc tggggtgagg gtggctgccc
    35101 acacgatact tacaacgact gatgttgcgt ctcatgggtc agcagcaggg cgattaacgt
    35161 caagccagcc atcgccgcca gatataaccc caccgcacct aacccgtagt tagtctgcaa
    35221 ccaggctgcg atatacggcg caacggaagc cccgagaatc gacgatacgt tgtaagagaa
    35281 cgatgctccg gtgtaacgca cttctgtcgg gaacagctct ggtaacagcg cgcccatcgg
    35341 accgaaggtc agccccatca gacttaaccc cagcagcagg aaggcaaaaa ccagaatcgg
    35401 gttgccagaa ccgagcaaag ggttaaaggc gaatagcgcg aacaggatga gcagcgtggt
    35461 gatgattacc atgcttttac ggcgaccaaa ggcatcagcc agtaatccag cgactggcac
    35521 catcacgcca aaaccaataa ctgccatcat caacatccac aacacttcgt tacgcggcag
    35581 gccaagccca actggcgcgg cggcggtact aaaggtcatg gagtagaccg tcatgatgta
    35641 aaacagcgta taggttgcca gcataatgaa cgtacccagt acggttacgc gaacatgttt
    35701 ggtcagcagc gtacccagcg ggatcttcac ctgttttttc gctttagcga ctttctcaaa
    35761 caccggcgac tcatgcagcg acacgcgaac atacaggccg ataatgacca gcaccgccga
    35821 gaagataaat ggcacacgcc agccccagct cataaactgc tcgtcggtca gtagccagga
    35881 aagcagcaag aaagtgccgt tggcaaagaa gaagccaatc ggtgcgccca gctgcgggaa
    35941 ggagccgtac aatgcacgtt tgcgcggtgg ggcgttttca gtcgccagca gcgccgcgcc
    36001 gccccattca ccacctaagc ccagaccctg accaaatcga gccagcgcca gcagcagcgg
    36061 ggcgaaaata ccaatcgtgg catagcctgg cagcagacca atcaccacgg tcgaaatccc
    36121 catcgttagc aacgaggcga ccagcgtcgc tttacgccca acgcgatcgc caaaatgacc
    36181 aaaaacggca gagccaatgg ggcgcgcgac gaaggcgatg gcgaaggtgg cgagcgactg
    36241 tagcgttgct gccgtaggat cgccctgcgg gaagaagata tgcggaaaca caataacggc
    36301 cgcagtggcg taaatgtaaa agtcgaagaa ctcaatggct gtgccaatga gagaggcgac
    36361 aaggacttta ttacgcgagt tgatcggcgt gtattcttgc tcgtggtcga gtgttgtggc
    36421 tgttgcttgc ataatctttt cttatttttt ggctaacgaa tagccatatt acgcacagca
    36481 aaagcgatat ttcaatgtgt aacaaatcgc ggaaatggtc aaaaaccagg caaaaagggg
    36541 ataatgttta ggccagtgat ttttcttcta tgaaatgctt cacataattt tataaatagt
    36601 ggataaaact ggtttcaggt taaataaaag ttaccaactg gatttatatg ctggggttat
    36661 gagttagaat gaaattgttt gctcccaaat agatggctga agaggctctg gggacgagtt
    36721 tcaagaggtt atgtgcgcat tatagccgga tgcgatgctg gcgcatctta tccggcctac
    36781 ggggagccag gccgaataaa atcgtatgcc ggataaggcg tttacgccgc atccggccta
    36841 ttttaaggct gcgttttccc tagcattcgc gggtcgtctt tatattcggc ggtggcaatc
    36901 cacgccgcgc aaaacagcgt caaacgggcg aagaagtaga aaaatgccat cagccccagc
    36961 acggaaccaa atgctgcgcc agagggggat ttcatcagcg atggcagggt gtaggtcatc
    37021 acgattttaa tcacttcaaa accaatagca gccagaaatg ttccgcgaat cagggctttt
    37081 ttgcgcggac ggtggcgcgg caaccgccag aagatccaga agaaaagcag atagttggcg
    37141 aagatggaaa tcgccaggcc aatcaatcgc cacgtcggtt tcagccactc aatgctgttc
    37201 aggtgcaggg cgctaataat catttgctgc gccgaaccgg caaccgaggt gatcgaaagc
    37261 gtcacaatca gcgcaatcaa caaaccaatc agtgaaataa aatcacgcag atattttacc
    37321 cagaacttct cctgatcttg cggcgagcgt tcccagacat cacgcgactg ggcacgaatc
    37381 gcttcacgca ggttacccat ccagttgatg ccggaataaa gtgccaccgc caggccgaca
    37441 agccctacag tcgtacgctg ctgaacggcg gtgttgatgg tgtttttcaa cgtggcggct
    37501 agtgtcggat cgctgatgtt ttgcagaatt ttgtcgaaga tatcctgtag caacatcgga
    37561 tgggaggcca gcacaaagcc ccctgcggca aacgacacca tcaaaatcgg gatcatcgac
    37621 aaaaatgaga aataggtgat agccgcacca aactggttac ccagccgatc gttaaagcgt
    37681 tctgtcgcgc gaatcaggtg cgcaataacc ggttggcgtt ggactttttc ggcagtggtg
    37741 gtgacggttt ccagcgcctg gctaactttg ccgcctttct cgctattatc caactgctta
    37801 atcggctcga gctccagatc ctgggtggga cgtttgatct cgttttcctg cgtcatccgc
    37861 tcttttgtcc ttctgtcgtg gtcattctta aaaagtatag tcggtcagtc caggtgctct
    37921 ttcattacgc cagccagcca ctgcataaac aggtttacac gccgggaaag ctcccgacgc
    37981 tgcgggtaaa ccagcgaaag ggagagcggc tcggcacggt agccaggtaa tacttcaata
    38041 agccgcccgg cacgcagggc ttcgcgcacg gcaatgcgcg ggatctgaat aatccccaga
    38101 ccggcaagac cggcggcgag atagttttcg ctactattta ccgtcaacat gccgccagac
    38161 ttaaaccact ggacgccatt aacgctggca acctcaaaac ctaacggatg tacacccagg
    38221 tgcggtgtat aacgcactat tgcgtgtgaa gtcagatcat cggggctttg cggataacca
    38281 aagcgcgtca gatagtgcgg actggcacag ttgaccatgg tcagtttgcc gaggggacgg
    38341 gcgataacgc catcttccgg taacgcgcca gtgcgtatca cgcaatcaaa accatcatga
    38401 agaatatcta ccggacggtc atggctactc agttccagct caattcccgg atgcagatag
    38461 agaaattccg acaggcgcgg cagtaacagg tttttcgcga ttcctggcgg gatgtcgatg
    38521 cgtaatttac cgctgatact ggtggcatcc tgttgaaaca gaccgtccag ttcgttgaga
    38581 ttactcaaca catcttttgc ccgttgataa taggtcatgc cttctggcgt gagtttgacc
    38641 cgtcgcgtgg tgcgctggag aagctgggtg ccaagttgat gttccagtgc ctgtatctgg
    38701 cgcgaaacac ttccctttgg caaagcaaag aaatccgctg cgcgggaaaa actttccagc
    38761 tccgcgactt tgatgaacaa ctgcattgcg tgaattttat ccatttcttt gaccgattgt
    38821 tgttcacagc gaaacagtaa cgcgcatatc gcattgttta ttgttttttc taacatctaa
    38881 tgccgggttt cacaatgttg tcatgacgaa tgtggcgatt tctcagcagg aaaagggcgg
    38941 aatagcttta tgagaaaggt gaatcccctt aaacagagcg tatttttcag tgattccctt
    39001 taaagtgaat agaggacgta ttggctattt gtgcttattt ttgtctcatt gccatttgtt
    39061 attttggctt gtagtcgcgg aatttcattt ttaattgcat gcgtactaac caccaatata
    39121 aggaaatcct caaaattgtt ttaaatagtg ccctcgacat gtaatttcag gcgtttagct
    39181 gtaactattt atatcggtaa ctattttcta cgcgtcatct ctgtagaatg gccaccgcca
    39241 aatggcgaat tcttattttt tctcgcgggt aatacccttc cactacttaa gtctttttaa
    39301 catattcagg tggagattca gttatgcaaa tagtcatgtt tgacaggcag tcaatattta
    39361 ttcatggaat gaaaatcagt ttacagcagc gtattccagg ggtgagtatt cagggggcca
    39421 gtcaggcaga cgagttatgg caaaagctgg aaagttaccc tgaagcctta gttatgctcg
    39481 atggcgatca ggatggtgag ttttgctact ggttgctgca aaaaaccgtg gtgcagtttc
    39541 ctgaggttaa ggtgttaatc acggcgacgg attgcaataa acggtggtta caggaagtta
    39601 tccattttaa tgtgctggcc attgtgcctc gtgattcaac cgtcgagaca tttgcgttgg
    39661 cggtaaatag tgcggcgatg gggatgatgt ttctgcctgg agactggcgg acgacgccgg
    39721 aaaaggacat taaagatctc aaatcgttaa gcgcccgcca gcgggagatt ttaaccatgt
    39781 tagcggcggg cgagtcaaat aaggagatcg gcagagcgct gaatatcagt accggaacgg
    39841 taaaagcgca tctggaatcc ctgtatcgcc gtctggaagt gaaaaatcgc acccaggcgg
    39901 caatgatgtt aaatatctcc tcctgaagag gataatttta cagccagcag gaagttagcg
    39961 gctggctgta aaaatattat ggttcgccgt acaaaccaat taaacggcgt accacaccgt
    40021 tagtccagcc aaacccatcc tgcaacggat actcgccacc gccgccttcg cgtggaacac
    40081 catcggcaat atggtatttt tcgatcagtt tgtgctgttc cagatagaac tgattcaccg
    40141 tcttcagcca gcttcgcgcg atttcatcac ccagaaggtc atcgccgtac attttaaatc
    40201 cctgaatcgc catccattgt aacggtgccc agccgttggg tttatcccac tgttcaccgg
    40261 tttcgtactc gcttgccaga atcccgccag gtgtcagtaa ccgactgcgc acggcatttg
    40321 ccagacgatc ggcctgttca tggttcgcca taccgacata gagtggcaca atggcggcag
    40381 cggaaaacag cgccagttgt tcgcgtcgcc agtcgtaatc gcggtagatg ccgttttcat
    40441 catcccagag gtaacgcttt accgcatcgc gacgggcact ggccttctgg cggaacagtg
    40501 cttctgtctc tttctcgcct ttcagcgccg agatgttggc gatggcgctc tccagtttaa
    40561 acaggaaggc attcagatcg atggggatga actgggtggt acgaatactc gccagacgac
    40621 cagtatcacg cagccaacgg gaagagtaat cccaaccgga ggccgccccc gctcgtaaat
    40681 cgcggtacac ctcgttgggc gggcgaccag aatgtttcgc ggtttcaacg tcctcaagcc
    40741 aggattcgtc acgcggcgtg tcgcgatcgt cccagtaacg gttgagcagc gatccgtccg
    40801 gcatccgcac aacatggcga taggcctgat tagggatcaa cgattctgca ccgtccatcc
    40861 agaaggcata ttccattttc aggtggtcga gatagcggcg cgcaccgcgt acaccatctt
    40921 cttcaaacaa ctccaccatc agcgcaaaaa ccggtggttg cgagcggctc aaataatagg
    40981 tgcggttgcc gttggggatg tgaccgtagt tttcgatcat ccaggcgaag ttatcggcca
    41041 tgcatttcag caaatcttcc cgaccacttt ccgccagccc cagcatggtg aaataggaat
    41101 cccaatagta ggtttcgcta aaacggccgc ccgggacaat atatgactgc ggtagcgcca
    41161 gcagagaaga ccacggaatg tgatcctgtg gttcgcgggt tagcaccggc cacagctggt
    41221 cgatatgctc tttcagggaa ttttgcgggt ccgatacata ctcgctggag tagacctccg
    41281 gcagccagaa gtgattttca acaaacttgc gcaagtcaaa atcacgatga cggcgcactt
    41341 tacggtagcg gattaaaata tccagcgggt ccattttggg tgcgcagtcg ggaaaggttt
    41401 tactgtcggg gaaaattttc gccgactgaa catgctcgaa cagttcgaga tagcgatcgg
    41461 caggcgtcag cgcatcagag gcgggcagcc cttcaatcat ctcgggttcc ggttctgcct
    41521 cgatcatctc atccagtttt aattcatacg ggtccagttc atagcagaga tcgacttcga
    41581 tcatcagttc gtctggatta gggttttgaa ttttctgatt gagcataagg atccaacctc
    41641 cgaaattcgt cgctaattat cggcttttct ttaagggtag tcattcgctc cgcctggtgg
    41701 gcgacaaagc gtgcggtgga tcacgtttat taacttgtca aaaaatataa cgcactaata
    41761 tcaatggaat ttataaaaat acggatttgg tgttgttttt cattttcaga acattcaatc
    41821 ctgctctcgc cgcaatcatg atatgccatt cctgatctgg gtaataggca caggctatct
    41881 tattgatagt ttatattcat gtaattgatt gattcctatc aaaaagcgta tctccctgtt
    41941 ttttatgatg gctttgtctc gcagagacgt ttgcagcatt ctggaaaatt ttctgggtca
    42001 caataactca ccaccgtgag ggaagaataa tgaaaatggt ctcacgtatt accgcgatcg
    42061 gcctggctgg cgtcgcgatt tgctatttag ggttatctgg ttatgtgtgg taccacgata
    42121 ataaacgcag taaacaggcc gatgttcagg catctgctgt cagtgaaaat aataaggttt
    42181 taggctttct ccgcgaaaaa ggatgcgact attgccacac gccttcggca gaattacccg
    42241 cctattatta tattcctggc gcgaaacagt tgatggatta cgacattaag cttggatata
    42301 aatcttttaa cctcgaggcc gtgcgtgcgg cactgctggc tgataaaccc gtttcgcaaa
    42361 gcgatctgaa taagattgaa tgggtgatgc agtatgaaac tatgccacca acgcgttata
    42421 ccgcgctaca ctgggcgggt aaggtgagtg atgaagagcg ggcggaaata ctggcctgga
    42481 ttgcaaaaca gcgcgcggaa tattacgcca gcaatgatac tgctccggag catcgcaatg
    42541 aaccggtgca gcccatcccg caaaaactgc ctaccgatgc gcaaaaagtg gcgttgggct
    42601 ttgcgctgta tcacgatccc cgtttatcgg ctgatagcac catttcatgc gctcattgcc
    42661 atgcgttgaa tgcggggggc gtcgatggca gaaaaacatc gattggtgtt ggtggcgcag
    42721 ttgggccgat taacgcgccg acggtattta actcagtatt taacgttgag cagttctggg
    42781 atggtcgtgc ggcaacattg caggatcagg caggtggacc gccgttgaac ccgattgaaa
    42841 tggcgtcgaa atcctgggac gaaattattg ctaagctgga aaaagatccg cagcttaaag
    42901 cgcagttcct cgaagtttat ccgcaaggtt tcagtggcga aaatattact gatgccattg
    42961 ctgaatttga gaaaacatta attacgccgg attccccatt tgataaatgg ttgcgtggag
    43021 atgaaaatgc gctgacggcg caacagaaaa aaggctatca attatttaaa gataataaat
    43081 gtgcaacttg tcatggtggt attattctcg gcggacgttc ctttgaaccg ttggggctga
    43141 aaaaagactt taactttggg gaaattacgg cggcggatat tggtcgtatg aatgtgacta
    43201 aagaagagcg tgataaattg cgccagaaag tacccggttt acgtaacgtt gctttaacgg
    43261 caccgtactt ccatcgcggt gacgtgccga cgctggacgg ggcggtgaaa ctgatgctgc
    43321 gctatcaggt aggcaaagag ctgccgcagg aggatgtgga tgatatcgta gctttcctgc
    43381 acagtctgaa cggggtgtac acgccgtata tgcaggataa acaataacta atttgatcgc
    43441 ccgaacagga atgtttgggc gatttttatt acgataataa agtctgtttt taatattatc
    43501 atgttaaatg tttatattat aaaaagtcgt ttttctgctt aggattttgt tatttaaatt
    43561 aagcctgtaa tgccttgctt ccattgcgga taaatcctac ttttttattg ccttcaaata
    43621 aatttaagga gttcgaaatg gaccagaagc tgttaacgga tttccgctca gaactactcg
    43681 attcacgttt tggcgcaaag gccatttcta ctatcgcgga gtcaaaacga tttccgctgc
    43741 acgaaatgcg cgatgatgtc gcatttcaga ttatcaatga tgaattatat cttgatggca
    43801 acgctcgtca gaacttggcc actttctgcc agacctggga cgacgaaaac gtccataaat
    43861 tgatggattt gtcgatcaa
//
LOCUS       DAJTMC010000009        42613 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.009, whole genome shotgun sequence.
ACCESSION   DAJTMC010000009 DAJTMC010000000
VERSION     DAJTMC010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 42613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42613
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.009"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(<1..451)
                     /locus_tag="OO130_000475"
     CDS             complement(<1..451)
                     /locus_tag="OO130_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HCR5364004.1"
                     /translation="MNNTDLIHLIKRFMRNEHKAVEEVIDSPLSEVANLIKILQSCQG
                     KVVFIGVGKSGIIARKLAATFASTGTPSFFVHGTEAVHGALGMVAKDDVVILISNSGE
                     TAEILATLPSLKKMGNYLISFIRSHHSSLAISCDLSVEIPVKSEADNL"
     gene            complement(420..1628)
                     /locus_tag="OO130_000476"
                     /pseudo
     CDS             complement(420..1628)
                     /locus_tag="OO130_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000350897.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
     gene            complement(1628..3208)
                     /locus_tag="OO130_000477"
                     /pseudo
     CDS             complement(1628..3208)
                     /locus_tag="OO130_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000810568.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
     gene            complement(3240..3887)
                     /locus_tag="OO130_000478"
     CDS             complement(3240..3887)
                     /locus_tag="OO130_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR5364005.1"
                     /translation="MIEHVGIEYIFVAEKIVHYAESNLEKKLNPSLLLILADHISNAI
                     SRVVSGIQINNVFLEEIKALYKAEYAISRDALTIINEQFSVQLPDDEIGFIALHILNN
                     YENSVDYESVRIIELSQKITELIEVVYNRRVDRSSFNYSRFMMHLKYFSSRVLCNEKI
                     KQKNIGDIYEQFLEKDFQLQRAIHEIERYLYATFKYDLILEEKLYLSIRIKVLMD"
     gene            complement(3884..4063)
                     /locus_tag="OO130_000479"
     CDS             complement(3884..4063)
                     /locus_tag="OO130_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HCR5364006.1"
                     /translation="MIIEKVMNNNCVQASVNGQEVIISGPGVGYNKNMVCRSLSILQT
                     GFFMSEMSKKINSTN"
     gene            complement(4323..5576)
                     /gene="amiC"
                     /locus_tag="OO130_000480"
     CDS             complement(4323..5576)
                     /gene="amiC"
                     /locus_tag="OO130_000480"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HCR5364007.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            5808..7139
                     /gene="argA"
                     /locus_tag="OO130_000481"
     CDS             5808..7139
                     /gene="argA"
                     /locus_tag="OO130_000481"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HCR5364008.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(7201..9027)
                     /gene="recD"
                     /locus_tag="OO130_000482"
     CDS             complement(7201..9027)
                     /gene="recD"
                     /locus_tag="OO130_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HCR5364009.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(9027..12569)
                     /gene="recB"
                     /locus_tag="OO130_000483"
     CDS             complement(9027..12569)
                     /gene="recB"
                     /locus_tag="OO130_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HCR5364010.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIATVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIDKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPKSVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNEKGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDRHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(12562..15450)
                     /gene="ptrA"
                     /locus_tag="OO130_000484"
     CDS             complement(12562..15450)
                     /gene="ptrA"
                     /locus_tag="OO130_000484"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HCR5364011.1"
                     /translation="MPRSTWFKALLLLVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISEQTFADWQQKAANIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFSSEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTQARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTTEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(15626..18994)
                     /gene="recC"
                     /locus_tag="OO130_000485"
     CDS             complement(15626..18994)
                     /gene="recC"
                     /locus_tag="OO130_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HCR5364012.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEHEDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPEWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRLWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGLSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDNI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(19007..19330)
                     /locus_tag="OO130_000486"
     CDS             complement(19007..19330)
                     /locus_tag="OO130_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCR5364013.1"
                     /translation="MSASLKNQQGFSLPEVMLAMVLMVMIVTALSGFQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRLQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(19315..19722)
                     /locus_tag="OO130_000487"
     CDS             complement(19315..19722)
                     /locus_tag="OO130_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HCR5364014.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAETDAQVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGNIVFSPSGWSDFCPLKERALCQLP"
     gene            complement(19719..20282)
                     /locus_tag="OO130_000488"
     CDS             complement(19719..20282)
                     /locus_tag="OO130_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HCR5364015.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGICTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVNASYSVTGFNL"
     gene            complement(20273..20743)
                     /locus_tag="OO130_000489"
     CDS             complement(20273..20743)
                     /locus_tag="OO130_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HCR5364016.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISVIREGTLWCLVSSAAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(20928..21722)
                     /gene="thyA"
                     /locus_tag="OO130_000490"
     CDS             complement(20928..21722)
                     /gene="thyA"
                     /locus_tag="OO130_000490"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HCR5364017.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYAPHPGIKAPVAI"
     gene            complement(21729..22604)
                     /gene="lgt"
                     /locus_tag="OO130_000491"
     CDS             complement(21729..22604)
                     /gene="lgt"
                     /locus_tag="OO130_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HCR5364018.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(22755..25001)
                     /gene="ptsP"
                     /locus_tag="OO130_000492"
     CDS             complement(22755..25001)
                     /gene="ptsP"
                     /locus_tag="OO130_000492"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR5364019.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGLPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPILLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDFAEAENLAQRSLEAQLATEVRHQVVAFMERRGM
                     GGLIRGGL"
     gene            complement(25014..25544)
                     /gene="rppH"
                     /locus_tag="OO130_000493"
     CDS             complement(25014..25544)
                     /gene="rppH"
                     /locus_tag="OO130_000493"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HCR5364020.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNTSAYRRKRG"
     gene            26229..26918
                     /gene="mutH"
                     /locus_tag="OO130_000494"
     CDS             26229..26918
                     /gene="mutH"
                     /locus_tag="OO130_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HCR5364021.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWTPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            26987..27700
                     /locus_tag="OO130_000495"
     CDS             26987..27700
                     /locus_tag="OO130_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCR5364022.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            27838..28056
                     /gene="ygdR"
                     /locus_tag="OO130_000496"
     CDS             27838..28056
                     /gene="ygdR"
                     /locus_tag="OO130_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HCR5364023.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            28164..29204
                     /locus_tag="OO130_000497"
     CDS             28164..29204
                     /locus_tag="OO130_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HCR5364024.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLNLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGGMRYLHLADKHDLPSIVTIQTPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKTAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(29236..30429)
                     /gene="lplT"
                     /locus_tag="OO130_000498"
     CDS             complement(29236..30429)
                     /gene="lplT"
                     /locus_tag="OO130_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HCR5364025.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVMGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(30422..32581)
                     /gene="aas"
                     /locus_tag="OO130_000499"
     CDS             complement(30422..32581)
                     /gene="aas"
                     /locus_tag="OO130_000499"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HCR5364026.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDASRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTLDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            32970..34001
                     /gene="galR"
                     /locus_tag="OO130_000500"
     CDS             32970..34001
                     /gene="galR"
                     /locus_tag="OO130_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HCR5364027.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIAANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(34008..35270)
                     /gene="lysA"
                     /locus_tag="OO130_000501"
     CDS             complement(34008..35270)
                     /gene="lysA"
                     /locus_tag="OO130_000501"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HCR5364028.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSVPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRSLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            35392..36327
                     /gene="lysR"
                     /locus_tag="OO130_000502"
     CDS             35392..36327
                     /gene="lysR"
                     /locus_tag="OO130_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HCR5364029.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASICAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(36314..37006)
                     /gene="ygeA"
                     /locus_tag="OO130_000503"
     CDS             complement(36314..37006)
                     /gene="ygeA"
                     /locus_tag="OO130_000503"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HCR5364030.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(37135..38550)
                     /gene="araE"
                     /locus_tag="OO130_000504"
                     /pseudo
     CDS             complement(37135..38550)
                     /gene="araE"
                     /locus_tag="OO130_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
     gene            complement(38865..39626)
                     /gene="kduD"
                     /locus_tag="OO130_000505"
     CDS             complement(38865..39626)
                     /gene="kduD"
                     /locus_tag="OO130_000505"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HCR5364031.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIEQVTALGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPIVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(39656..40492)
                     /gene="kduI"
                     /locus_tag="OO130_000506"
     CDS             complement(39656..40492)
                     /gene="kduI"
                     /locus_tag="OO130_000506"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HCR5364032.1"
                     /translation="MDVRQSIHSAHAKTLDTQGLHNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(40779..41960)
                     /locus_tag="OO130_000507"
     CDS             complement(40779..41960)
                     /locus_tag="OO130_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HCR5364033.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PCTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            42215..>42613
                     /locus_tag="OO130_000508"
     CDS             42215..>42613
                     /locus_tag="OO130_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCR5364034.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSAGEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLG"
BASE COUNT        10842 a        11398 c        10106 g        10267 t
ORIGIN      
        1 ctaaattatc cgcctcactt ttgacgggga tttcaacaga taaatcacaa cttatcgcaa
       61 gcgatgagtg atgactgcgg ataaaagaga tcaaataatt acccattttt ttcaaactag
      121 gcagtgtcgc caggatttcc gccgtctcac cactgtttga aataagaata acaacatcgt
      181 ctttcgccac catcccaagg gcgccgtgta ccgcttccgt accgtgaaca aaaaacgagg
      241 gagttccggt gctggcaaat gttgcagcaa gttttctggc aataatgcca gatttcccaa
      301 caccaataaa aacaactttt ccctggcaag attgtaatat tttaattaaa ttcgcgactt
      361 ccgaaagggg ggaatcaata acctcttcta ctgctttatg ttcgttacgc ataaaacgtt
      421 taataagatg gataagatcc gtattgttca ttttcttttt cctgattaat atttttaatt
      481 acactcgcaa ggcgcgcaag ccccatatat aatttttctc gactgcaggc cagattaaga
      541 cgcagatagc cctctccttc agtaccataa acatgccccg ccataatacc tactctccct
      601 tcgttaatta atcgacggta aaatttatca aagggataac caagggccga gcaatcaacc
      661 cacgaaaaat agcagccctc agctggccat acgatcaatt ccggaatatt ttcatttaaa
      721 tactgcaagg taaattgaat gttagaggca atatgtacct ttaattactt taaccattct
      781 tcacattcat tataggcaac aatggtcgca attacattca gtactgatgg ggagtttacg
      841 aaatcgcgat acctcgatat cgttctgaac ttatgattaa aattatcgtc agttgaaatc
      901 atatatgatc cggttagcgc aggaacatta aatgttttcg aaatcgaatt aaacaccacc
      961 actttgtcat cttttattgt tagcgctgac gtgaaactat gattaaaaac aaaatcacta
     1021 tgtatttcat ctgaacataa ccaaatatta tgccttgcac agcatgctgc gattctgtga
     1081 agatcgtctt ccgaccagac cgttcctgtc gggttatgag gattacataa taacaccatc
     1141 ttcgcgcttt taattttaag ctcaaagtct tgccagttga taacgtagcc tctcccattt
     1201 tctttcatca aagaggttgt tagcatgttt cgaccattat gcttaatcac atcaaaaaat
     1261 gcgttataaa caggcgtgaa aatgagaata ttatctccat tcaatgtcaa tatttctatt
     1321 gccttagata ttgaatacat cacgcttggg ctataggtga tccattctgg ttgaatctct
     1381 gttttatatt gttgatgata ccaattgact atcgaattca gatagtcatc atgctgccaa
     1441 cgactgtacc ctaaaatcgg gtgttccaga cgttttttta atgttctgat gatagcttcc
     1501 tgtatcggaa gatccatatc tgaaatagag aatggtaata catcttttac accaaatctg
     1561 tcttcacaaa agtcccattg tgtgcaatat gttcctttac gagaaattga ggtattaaaa
     1621 atatccatca acatatcccc atgtaattat ccatatcccg acgtagggcg atgacttttg
     1681 taccgataat gacttgtaag gtattttgat caagtttcac aaccgcaatt cctccggccc
     1741 tttttattgc agcctcattt atcaatccca tatccgcgat agttaaacgc aaacgagtta
     1801 cacaattatc cagtgattta atattttctt taccacccag agccttaacc atttgctccg
     1861 ctgcctgcaa ctctgattta tcatgaactg ggatgttagc tattgcagtt tcttcgtgag
     1921 cgttcagcgt gacaggactg gactcttcac ggcctacagt catcaggtta aatttgagaa
     1981 tggtccaacg gaacacaaag taataaatga agaaataagc cacttcaaca cataccgcta
     2041 atggccatcc cgttcttgtg ccacgtaaga caccaaagct aataaattga atcagatcgc
     2101 cagtaaagcc catcttcacg cccatatatg gcagaaccat gttagccaat ccattcataa
     2161 aggcatggaa gacaaacaac agcgggctga caaataagaa cagaaattca atcggctcgc
     2221 tgacgccgcc aataattacc gtgagcacac cggaaatcag caatgctttg atagcccttc
     2281 tgttttgagc atgagcagtg cgatacatcg ccagcgcggc tccagccaga ccatactgaa
     2341 tcatcagttt gccctgctcc attttccccg ccaaatccag cggaatgacc gcattctctt
     2401 tgacgtatgc cataaacatg ttcagggtgc cgtaatactc ttcaccgcca atcactgccg
     2461 atccgccgat tggggtaaag cggaaaactg atgtcaccaa gtgatttaag ccaaaaggta
     2521 tcgtcacgcg ttcggcaaca gcatagaaga aataaccaac aggaccggaa gttgaaatcc
     2581 agtgaccaat gagcatgaaa agattgaaaa atggcggcca gataaaagga atgatcagac
     2641 caaatccact catcacaatc agtgtaatga taggcacaag gcgtgggcca ctataaaacc
     2701 ctaattattc aggaatacgt aagttaacga tctttttata catgctggcg actaataacc
     2761 ctgccacaat cccacccaac acgctggtat tgtaggactg gatcccgagt atgatggttt
     2821 gcccatgtgt cgacatttgg tcagcagcta ccaataagtc gtgctgttta agataaaagt
     2881 tcgttccaag atgcatcgcc ataaagccaa ttaagccaga aaaagcgcca taggctttat
     2941 catctttctc ttttaataat cccaaaggta tagctatcgc aaacaataca ggtaaattaa
     3001 cgaaggcaaa caaaccaagg ctaacaataa aatcaagtat ggttttaatt aacggaatag
     3061 ccaacactgg aatgaatttt gccatatcat cgctggctaa accacttccc agccctaaca
     3121 tcataccaca tacacttaac agagcgatgg gatacataaa tgccttcccc aggctctgaa
     3181 aaaaactcca ggatttcttt tgtttcatac tggttatctc atgaaaatgt tatgtataat
     3241 tagtccatta atactttgat gcgaatagaa agatatagtt tctcttccag aatcaaatca
     3301 tatttaaaag ttgcgtacag atatctttca atttcatgaa tggcacgttg taactgaaaa
     3361 tctttttcaa gaaactgctc atagatatca ccaatatttt tctgttttat tttttcatta
     3421 cataacacac gactagaaaa atatttaagg tgcatcataa atctggagta gttaaatgaa
     3481 cttctgtcca cccttctgtt ataaacaact tcaataagct ccgtgatttt ttgagacagc
     3541 tcaataatcc ttactgattc ataatcaact gaattttcat aattatttaa aatatgcaat
     3601 gctataaaac caatctcatc atccggtagt tgaacgctaa attgctcatt aatgattgtt
     3661 aatgcatcac gacttattgc atactctgct ttgtacaacg ctttgatttc ctcaaggaaa
     3721 acattattaa tttgtatgcc tgagacgact cgggatattg cattcgaaat gtgatcagca
     3781 agaatcagta gtagcgatgg attgagtttt ttttcgagat tactctccgc ataatgcact
     3841 atcttttcgg caacaaaaat atactcaata cccacatgct caatcaattt gtagagttta
     3901 tttttttgct catttctgac ataaaaaatc cggtttgcag gatgctcagg gaccgacata
     3961 ccatattttt attgtaacca acgccaggcc cagaaatgat aacctcctgt ccgttcaccg
     4021 atgcctgtac acaattattg ttcatgactt tttcgatgat cattccatgg ctccccaaaa
     4081 aaaaggcaag gcatgtcaga agtaataatt acttcaaaca ttgccttgcc tgatttaaca
     4141 gttgcaagat aattaactaa tgcgtatacc ttacacggaa ttgtgcttaa taaaagagat
     4201 gacagactta ttttttaagc cgatattcaa atattcgaca gcagtcacaa aaccatcgtt
     4261 caacatttat gtaaataata tttattcaat gaaacataaa ttcattgaat aaattaaaaa
     4321 catcatcccc ttctcgccag cgtcgcccca tcggcaaaat acgctttaat ccccgcaaga
     4381 atagactccg caacttcctg ctggaaagtc gccgttttca gtttacgctc ttcctcaacg
     4441 ttactaataa acgccgtttc gaccagaatg gagggaatat ctggtgcctt tagtacggca
     4501 aacccggcct gttcaacttg atttttatgc agcttgttga ttttaccgag cttattcagt
     4561 accgctttac caaacttcag gctgtcggca attgtcagcg actgcaccat atcgaacatg
     4621 gtgtggtcga catagcggtc accgcttttg ctcacgccac caatcaagtc cgaggcgttc
     4681 tgggtttgtg ccagatattt tgccgcagta ctggttgcac ctttggttga gagcgcaaac
     4741 acagaggaac cgctcggctg acgactggta aaggcgtcgg catggataga gacaaacagg
     4801 tcagcacgct gtttctgggc ttttgctacg cgcacttgca acggaatgaa gatgtcttca
     4861 ttgcgcgtca tgtacacctt catattgccc tctttctcga tcagagagcg cagacggcga
     4921 gctatttgca ataccacgtc tttttcgcgc gttttgtatt tccccaccgc accggagtct
     4981 tcgccgccgt ggccagggtc aagcataata acaataggac gatcgcgccc tgctttaccc
     5041 ggttgtggac cactttgtgc tggcggcacc tgcttttcga ggtcgccttt gttgtaatcc
     5101 tccagcagcg ccagcagcgg gtcctgcata tcctgtgcat tggccggata gaggtccatc
     5161 accagacgct ctttaaaccc ggcgaccggc gcaagggcaa acagctgcgg ttttacgttt
     5221 tgctttaatt caaaaaccat ccgtacggtc tgcgggtcaa attgcccgac gcgcgccgac
     5281 ttgatgaacg ggtcgtctgc acgaatttgt gccgccatcc ccttgagtac cgagttcagg
     5341 tttacatctt cgatatccac caccacgcgt tcagggttac tcaacgcgaa ctgcttatat
     5401 ttcagctgac gatttgattc taccgtcaca cgggtgtagc tggacgcagg ccagacgcgc
     5461 accgccacga cctggctgac cgcagccaga ctgacctgac ttacgctcaa tagccacatg
     5521 gcacccgcgc cttgcagtaa acgacgacgg ctgattgcag tgttggatcc tgacatgcct
     5581 ctcccgagca aaagaaatct aagctgtgta acaagtaaac gactaatttg accggaaact
     5641 ttagcgaaag acgcataatc tgtcatctaa taaacggtaa acattctttt tatattcacg
     5701 gcattactga taaaaaagtc gctctcgcat aaaatttaca cttgcaccct gcgaaaaaac
     5761 agaataaaaa tacactaatt tagaataatc atgcaaaggg gtgtgccgtg gtaaaggaac
     5821 gtaaaaccga gctggtcgag ggattccgcc attcggttcc ctatatcaat acccaccggg
     5881 gaaaaacgtt tgtcatcatg ctcggcggtg aagccattga gcatgagaat ttctccagta
     5941 tcgttaatga tatcgggttg ttgcacagcc tcggcatccg tctggtggtg gtctatggcg
     6001 cacgtccgca gatcgacgca aatctggccg cgcatcacca cgaaccgctg tatcacaaga
     6061 atatacgtgt gaccgacgcc aaaacactgg aactggtgaa gcaggctgcg ggaacattgc
     6121 aactggacat cactgctcgc ctgtcgatga gtctcaataa cacgccgcta cagggcgcgc
     6181 atatcaacgt cgtcagtggc aattttatta ttgcccagcc gctgggcgtc gatgacggcg
     6241 tggattactg ccatagcggg cgtatccggc ggattgatga agacgcgatc catcgtcaac
     6301 tggacagcgg tgcaatagtg ctaatggggc cggtcgctgt ttcagtcact ggcgagagct
     6361 ttaatctgac ctcggaagag attgccactc aactggccat caaactgaaa gctgagaaga
     6421 tgattggttt ttgctcttca cagggcgtca ctaatgacga cggtgatatt gtctcagaac
     6481 ttttccctaa cgaagcgcaa gcgcgggtag aagcccagga agagaaaggc gattacaact
     6541 ccggtacggt gcgctttttg cgtggcgcag tgaaagcctg ccgcagcggc gtgcgtcgct
     6601 gtcatttaat cagttatcag gaagatggcg cgctgttgca agagttgttc tcacgcgacg
     6661 gtatcggtac gcagattgtg atggaaagcg ccgagcagat tcgtcgcgca acaatcaacg
     6721 atattggcgg tattctggag ttgattcgcc cgctggagca gcaaggtatt ctggtgcgcc
     6781 gttctcgcga gcagctggag atggaaatcg acaaattcac cattattcag cgcgataaca
     6841 cgactattgc ctgcgccgcg ctctatccgt tcccggaaga gaagattggg gaaatggcct
     6901 gtgtggcagt tcacccggat taccgcagtt catcacgggg cgaggttctg ctggaacgca
     6961 ttgccgctca ggcgaagcag agcggcttaa gcaaattgtt tgtgctgacc acgcgcagta
     7021 ttcactggtt ccaggaacgt ggatttaccc cggtggatat tgatttactg cccgagagca
     7081 aaaagcagtt gtacaactac cagcgtaaat ccaaagtgtt gatggcggat ttagggtaag
     7141 cgatgaaaat tgtcggatgc gacatgcgta acactcgtgc gtcgcatccg gcaattacgt
     7201 ttattcccgc gaactaaaca acgccgccag accactgcgc cgctcagtac gagtggcgat
     7261 tgccgcactt aatatacgct catcggcata cagcgacaga cggcgacgcg cgcgggtcac
     7321 cgcggtgtat accagctctc gcgttactac cggcgtgcgt tggctcggta aaatcaacgc
     7381 cgcatggtcg aactccgatc cctgcgattt atgtaccgtc atcgcccacg tagtttcgtg
     7441 ctctggcaag cgactcggtt gcacagactt aatattgccg tccggcatcg caaaccagac
     7501 gcgcgtcccc tgcccgcgat ccagcgcaat accgatatcg ccattaaaca acccaagcgc
     7561 gctgtcatta cgggcaatca tcaccggtcg accttcgtac caacgagagt gcggatgacg
     7621 atgaattttg cgcttctgtt gcataaactg ctcaattcgc tcattcagtc cagccacgcc
     7681 aaacggccct tcccgcaggg cgcacaaaag ttggtactca ttgaacgcct gaatgattaa
     7741 atccggctcg gcacgcgctt gcagcagatc cagataacgt ccataacccg caagagcttc
     7801 ctcaagcatc gcaatataat cttcgccgct ctgtaaaagt cgtttttcga tatcagtaaa
     7861 atcctgctga aaaacggttt tcactgccgt tttatcacca cggttgatcg ccgcagctaa
     7921 ctgaccaatg ccagaatcgc tgccgaaacg atagcttttt tgcagcaggc agagactgtc
     7981 gcgcaaagat gccgcttctg tgccggttcc tgccggaacg tgagtccccg tcaggcggct
     8041 tagctgcctg gcacgctcgg cggtaaagcc cgcgttggca taagcgcaga tatcgcccag
     8101 cacagcccca gcctcaaccg aggccagttg atcacgatcg ccgagaaaga tcactcgcgc
     8161 atgatcgggc aaggcgtcga tcagtctcga catcataggc agatcgatca ttgacgcttc
     8221 atctaccacc agcacatcaa gatgcagcgg gttaccggca tgatgacgta aacgctggct
     8281 acccggctgc gcgcccagca atcggtgcaa agtgctggca tcttccggaa tgcgtttctt
     8341 ttgttcatcg gtcagcggta actgtcgcaa agccttgccg agagattcgg ttaagcgcgc
     8401 ggcagcttta cccgttggtg cagccagacg gatacggcag cgttcgccgt cggccatttg
     8461 aattaacgct gccagcaact tcgccacggt ggtcgtttta ccggtgcctg ggccgccgga
     8521 aatcaccgag atccgccgcg tcagcgccac tgccgctgca actttttgcc agttaatttc
     8581 atcgcttact ggaaaaagtt tgtccagggt ttgcgccagt agagcttcat caacctcaat
     8641 ggcatgattc acctcattga agaagcgcgc taccgtccgc tcgttacacc acatgcgatt
     8701 caaataaaga cgatcgccac agaggatcat cggcgtgggt tcatctcccc tgctgaccgc
     8761 ttgagaagcc agcaagcatt cttcccaatt ttgtagctca ccgatttcac tgacacaggt
     8821 cgccaacagc ggatgcgacg cctcgttatt ttccagtcgt gaaagcggca aacagacgtg
     8881 tccctctccg gcatcatgac ttaacagtgc cgccgcgagg gtgacggcag gatgttcatc
     8941 tcccgccacg gtcagggcaa attgcacatc cagcgggcgt agctgtttgt gctccacagc
     9001 ttccagtaat tgcttttgca atttcattac gcctcctcca gggtcatacc ggcaaacatc
     9061 tcatccatca gggcaatcaa cccggcgttg ggtcgggttg tgtaaatccc ttgttgcgga
     9121 tgttctttat caacgccacg caggaacaga tagataacgc cgccaaagtg acgatcatag
     9181 tcgtaatcag caatgcgatg gcgcagataa cgatgcagcg ccagggtata aagctgatat
     9241 tgcagatcat agcggtgtgc ctgcatcgct gctgccatag cctgttgggt gtaagccgaa
     9301 ctgtcttcac ccagccagtt ggatttatag tcgagcaggt aataacgccc ttcgtggcgg
     9361 aatactaggt cgataaagcc ttttaacatg ccgcgtacct gcatgaactc cagcggcggg
     9421 cagccagcgg atagcggatc aaactggcga attagtgcat caagctggct agcgatgagc
     9481 ggttcgctaa tcggcaggta aaactccatc tccacctgtt tatcgcggtc ggaaagctga
     9541 ctcaggctaa cccccttttc attgagaggt gcctggagga cagccgtgat ccactcggtc
     9601 aacaccggtt cccactgcga ttcaaagccg ccgagttcca gtttttcctg tacccagttc
     9661 gggtcaaccg gctgggtaaa atcgaggtct tcaaacaaac tgtgcaagaa tgtccccggt
     9721 gacgcaccgc gcgggaactg atgcggtgtt aacgtcggtt cttcaacgac gctgaccacg
     9781 cctgcggcat caacatccag ccgtggcatc agatcctgag cgataccgtg accacgctgc
     9841 tgcaagccgg agtagctggt gacacgccag ttatcgccgg gcaatcgttg tagtgtcctc
     9901 gcattcagtt ctgcagttaa agcatcatta acctgccagg gctggttatc accagtttgt
     9961 gccgtttgcc aggcaatatc atcatcgcat aacgcttcaa tacaggtgcg aagccctgcc
    10021 gcatcttgcg gttccccttt ttgcagcaaa cgcccgagcg cactttggtg gacgtcggtg
    10081 tcaccttttt tatcgccacg acggcgcacc agcggtgcaa cgccgagact gcaatgccaa
    10141 accgaacgcg tcagcgccac gtaaagcaaa cgcagatctt ccgccagacg ttcgacctcc
    10201 gcgaggtcga cgctttttgg cgcagcatta agatccaaaa ctgcctcaaa cgagtggcga
    10261 tcgtgataaa acgcctgatc ctggacgcgg aaattggtaa taaatggcaa ccagaccagc
    10321 ggatattcca gccctttcga tttatggatc gtgacaatct gcaccagatg tttatcactt
    10381 tcgaggcgca tttgttggct ggaggcatta ctgtctggct cgaggatatg ttgcgataac
    10441 cagcgtacca gcgcatgttc actttccagc tgcgttccgg cttcttgtag cagttcgctg
    10501 atatgcaaga tatcggtaag acgccgctca ccgcctgccg ttgccagcaa gttttctgca
    10561 atgttacgcg ccgacatcag cgcccgcagc atcggcataa cgccacgttt gcgccagatt
    10621 tgccgataac catcgaactc ttcaactacc gcatcccacg catgttcgtc attattcagc
    10681 gtttcgatat ccagcgcgtt cagccccatc attgacgttg ccagcgcact acgcagggtg
    10741 ttctcacgtt cgggcgtcat caccgcctgc aataaccaaa gcatttcctg cgcttccaga
    10801 gtttcaaaaa cactgtcgcg gttcgaaagg taaacggaag ggatttccag caacgttaag
    10861 gcatcgcgca cctgggcggc ctcctggcgg ctgcgcacca gcacactgat gtccgaagca
    10921 cgcaccggac gcgcgtcgtc gccgttcatc agcaacgctt cgccccgctg tccggcttgt
    10981 agccagtcgc ggatttgcgc agcacatacc tgcgccatgg tactttgata gtcgccaacg
    11041 ccgcagcttt cgccttccat cagccacatt ttcatcgcag gctgcgtttc acctttaaat
    11101 acaaaacgta acgcctgatt tttcccggct gatttcactg gaataaacgg tatttcgcga
    11161 aacatgaacg cgtcatcagt ctggctgaaa agcttgttca cactgttcac cattccaggt
    11221 gcggagcgcc agttggtgtc taaagtgtag tgggcgtgaa cttcactacg cgccttcata
    11281 taagtgaaga tatccgcacc ccggaatgca tatatggcct gcttcgggtc gccaatcagc
    11341 aacaatgcgg tttccggctg atggtgccag atacggcgaa aaattcggta ctgctggggg
    11401 tcggtatcct gaaattcatc gatcattgcc accgggaatc gcgtacggat cgccgctgcc
    11461 aacacctcac cgctttcgct acgcagcgcg gaatcgagcc gacttaacat gtcatcaaaa
    11521 cccaattcgc cacggcggcg tttttcacgc gctactgttt cgcggatctc agccaatgcg
    11581 cgggtgatca ccagatcgcg gatcgacaat ggttctgcaa gcagttgatc gatcgcctca
    11641 aacagtggat gtcgcggggt ttccccccca gctttcgtgc gatcttctaa gaaacgctgg
    11701 gagaattttt ccagcgactc cggcaactga taactgttgg tctcttcttc tgcccaggcg
    11761 ctgatcttgt cgatccattt agcctgattg ctacggttaa acttgcgtcg atcaatacca
    11821 gaagattcga tcagcgcatc cagttcaccc actgcgtcgc gccactgctg ttttaccgta
    11881 gcaatacgcg ccacaatttg cgcgtggcgg gaagccagcg tttcatcatc gggcggcggt
    11941 gctttgataa ccggcgcttc gccttgcaga taacgattaa tatcgcgcag caacgcctgc
    12001 ggccctttcc aggtttcaaa gacgacctgg gctatttcac gcggcagcgg gtagcagtgg
    12061 cgacgccaga aatcggcgca ggcctggtaa cgtagcagag actcatcttc aatcagctgc
    12121 tgctcaaaca gcatgccgga ttcaaaggca ttcaggttga gcatgcgctg gcaaaaaccg
    12181 tgaatagtaa agactgccgc ttcatccatc tgccgttcgg ctaacaacaa ccactgcgcg
    12241 gcttgcgctt tatcgtcgat ctcttccagc agacgttcgt acagtggatt gtcggtagtt
    12301 tcacgcagac aggcgatgcg caactcatgg atattgctac ggatacgacc acgcaattct
    12361 gccgtggcag cctcggtaaa ggtgaccacc agcagttctt caacggtcag cgggcgggga
    12421 aaggcggcgg aaccgcctag tccaagtaac aggcgcaaat agagcgccgc aatcgtaaag
    12481 gttttgcctg tgccggcaga ggcttcaatc aggcgctcac cctgtaaggg caagcgcaaa
    12541 ggatctagtg tctcggcgac atcactcatt cttttcactc atcaggggca ttgtttgctg
    12601 caacgcgctg acgttctccc acactttcca gccttcaggg tgtacatatt cggctttccc
    12661 gttctggctg ccggaaatct gcgacagaat agccatgcct tgcggctcga ccaccgcctg
    12721 atggaagaaa tcagcaagtt tttgcggcgt cagcagtttt atctgggcca cgattttatc
    12781 acgcgaatcg aagcgcatat tgccgcgatc gaaatcttta cttaacttcg atgcttcttc
    12841 gccgagcgtt tgcggtgcct gcagcatctg ggtaattacc gcctgctgga tttgcgcaaa
    12901 ctcatctggc ttcatcgctc gcaattttgc ctctgtggtt gggaaaaacg ccttgtaacg
    12961 ctcccacaag aatgaaggct gtttatcatt gctttgcaaa aggaagccca tgccccactg
    13021 acgccccacg ctcattggaa acgcaaacac ggcatagccc aattgttctt cggtacgcaa
    13081 ctgattgtag aaccacggct gtacgatctg ccccaacaga gagctatagg ctgagctggt
    13141 gtattcatcg tagccagtcg gtacaaatac cgctgccagt gcggagtcgg tgctgttacc
    13201 ggctttttca aagatgacgg attgtttttt atcgaccact acatctttgt ttcgacacca
    13261 ctctgaacca tcagcgccca actgtttttg cacatcgcgt gcctgcgttg ttgcctgggc
    13321 ctcggtcatg ttgccgataa ccataaactc tggtcgagcc cctgatttta aggcgtcgcg
    13381 ataggccagc acctctttca acgtaatgga gggcaaaatt ttacgccgtt catctcgcga
    13441 gaagtacggc acttgcgaga gcatctgcgc gggcataatc gcctgctcaa acgctttacc
    13501 cttttctgcg gaatccatca tctggttata ccaagacttc gcctgctcaa gctgatcttc
    13561 cgtagcggta tagctaaagt acccctcgag caatgcctgg aacagctgcg gcagacgctg
    13621 ggtgtaacca ttagcattaa ccataaggcc gttgttagcg ttggtggaaa aacttatgcc
    13681 accaaccgac gcctggttgc ttaactgatc aagcgccagc cctgcgagat aatcattgag
    13741 cgcaaacatc acctgattgc gggcgctgtc catggctttc ggattacgca aaatcaggct
    13801 gacatcagct ttgggttcgc tggaaaaata acggcttggc gcatacacca cgcgcagatt
    13861 cgactcatca acaatcagct ctggatggtc atatttcttc tctgacttaa tcagcgagaa
    13921 atcgtcagga atataggggt taagctccgg taaggagagc gcaatattgg cagctttttg
    13981 ctgccagtca gcgaaagttt gttcgctgat tttatcgacc tgatacggcg catcgacaaa
    14041 ataagccgtt ttattgtgcg gctctttcgg gctgatatac cagatacgcg cattctgcgg
    14101 cgtcatcatc gccagacgtt cttttactgc tttagcatcg taccgatcgg caatattgac
    14161 tgcatccagc gtatgctcaa ccggaacgcg aatcatggta tctgccagcc attcgacgta
    14221 atccatatca cgggtgatcg acggataacg gaagtcgata tccagcacat tcgccagttc
    14281 atcgaagtat tgtttatcaa tgcctttttc acgtaacaga ttgagatagc taaaaattgc
    14341 cgccacaacc tgatcgcgat tagccaggcc tttatcggtt aaagacgcag agatcgctaa
    14401 tacgccgctg ttgccgttga cgataggatc ggagttggcg ctaatgccct caactaatcc
    14461 ctgcttttgc agccagtcgg aaagtgtacc tgggctgcga ttaccaatca gataggtaat
    14521 caattcatcc gttttactac ggaacttcgc tgagttgtta tcgatgcgaa actcaacgcg
    14581 cagaacttta cgcggcagtg ccgggacgta atgaatgata atgccctttt gcgcgtcggt
    14641 gactaccggc acggtgattt ccggtttttt gctctctttg ttcggcacgc gaccaaaggt
    14701 gtccgccgcc atttttgcca actccggcag cggtttattg ctgtaaataa ccgccttcat
    14761 cagattggcg gaatagtact tctcgtggaa atctttcagc gcctgctgca ccggattacc
    14821 aggtttatcg cttaaagttt cgaggttacc accagaaaac tttgaaccgg ggtgtgccgg
    14881 gttaatggtt tctgcgctga cctgtgccat gcgcatcccg tcacgcgtac gcgccatggt
    14941 taattcagcg ttcaccgcat tacgctcacg ttcggcatat ttcttgtcga gcaaaggttc
    15001 agcaatagca tcggccaggc ggtctaccgc accaggcaag gcgtcgttct caacttccag
    15061 atagaaagcc gtgcgatacg gcgcagtgct ggcattgtga ctaccgccgt gcattttgag
    15121 atattcggcc agactgtcag cctgcgggta ctttttcgac cccatcagac tcatatgttc
    15181 aaggtaatgt gccagcccct ggtacgcctc gggatcttcc agcgacccaa cgggcaccac
    15241 cagcgccgag agcgatttaa ctgcctgcgg atcagaaacc agcaagacca ccataccgtt
    15301 atccagacgt atagcctgat actggcggtt atctttatca cttttacgga tggtttcctg
    15361 aatcggctgc catcccgttt ctgcctgact taagggtgcc caaagggcaa ctaacaacaa
    15421 taatgctttg aaccaagtgc tgcggggcat tcacggacct cataatcaac ttaattttct
    15481 gtccagattc aacacgttaa cgcaaatcat ctgccagaat ttaatcttgt gctgcacgag
    15541 tcagcctatg tttatataac catcagtccg tgactggtgc gcatcataaa gtaagcggat
    15601 agattgcgca atttttatac agcactcatg actgattaaa gcgaaacagc ggtaacagga
    15661 aacgttgcga ctgttcaacg atggcctcca ttgtctctgg tgttaattgc cgccagagcc
    15721 tttgatacca gatattatca ccttcgccac gcaccatcat gttgccttcg taagcctgaa
    15781 ggaatttcgt acgggctttt tgcaacgtgg aatcgtcatc cagcatggca tcgttttgcg
    15841 cgtcataaca ggtttttagc cacgcgccgc cactttcagg taacaccagc aatggcgcgg
    15901 acatcccttc acgatacccc tcaatcagtt gtgagaggta atgcaaagcc tgttcggctg
    15961 caagcggcgg aaaacgccac tcgccgtctt tgcgtagaaa aaggcgactt tcaccattac
    16021 caccgctggc acagtagaca aggtgttcca gccaaagttg cattccctgc gccacactta
    16081 ataaagaggg acgccagcgc aacaggccat ccggctgcac ctgcggcaac cagccagtta
    16141 tctgcacacc gttgcaggcg agatcaattt ccatactctg ccccggctgg cgacaggcaa
    16201 tgactctgtc ggcaagctgc tgcatctcct ggcactgagt ttcccagaaa atttcaccaa
    16261 aagcgccata cggtaaatcc cctgccgccc ggaagcgacg gaacaagcgt tcggcatcat
    16321 cctgctcaac cagtgcattc aataactgct gattgatttg ataacggcta agcccttcca
    16381 gaataaatgg ctcggtgtcg gggatttcgc tgtcttcggt acggaagttc acctgcaaac
    16441 gcatctggaa aaatgcccgc accggatgtg cccagaatcg ttgtagcgtt tccagcggca
    16501 cggtttccgg taaggtaaac ggcagcggct gaacaaattc agaatgtgct ttaccagcct
    16561 ggctggccgc aggtagccat tcacgagcat agctttgtcg ttcgcctggc tggtagtttt
    16621 gtggatcaaa cggcatccgg gtatggaggc aggtaagatg cgcttttacc cttgcctcgc
    16681 tttcatcaca gttgagcgct tcatcgcccg gtagataatg actttgcccg atgtagtcga
    16741 tcagttcctg caccagtacc gacgggaaac gttcactgtt atcctgaatg gaaagcccga
    16801 tataactgat atagagtttt tgctgcgcgg aaattaacgc ttccaggaac aggtagcggt
    16861 cgtcatcgcg acggctacgg tcgccacgct tcggtttctg gctcatcagg tcaaagccca
    16921 atggcgcaag ctgacgtgga taaacgccgt cgttcattcc cagcaggcaa accactttga
    16981 acggaattga acgcattggc atcagagtac aaatgttaac cggtccggcg agaaaacgct
    17041 ggctgatacg ttcttgatcc agacgctgtg ccagttcatc acgcaatagt gacagcggca
    17101 ccgcgtcgcc atactgcgcc cctaaacctt cggcgataat cgcctgccat tgttgttcga
    17161 tcagcgtcat cgccgcttcg gtttccgcat ccggcaggaa gaaggcgttg agcatatcgc
    17221 gacaaaccgg caaccactct tctagcggac gctcctgtgc cagcccgcga cgccagatgt
    17281 ttagctgcat tagcagtgaa gccagatgcc ccaccagttc tgcaattaag ccgctcgact
    17341 catcataagg tagaaccgat tgccactcgc cctgcgcgct ctccatcgcg tagcccagca
    17401 acatacgcgt caggccaaat cgccaggtgt gttgtccggt ggcggggagt tccagctcgc
    17461 gaacgttgtc gtcatctatc ccccaacgaa tgccggattc gttgacccac aggcgtaaat
    17521 aacgcagccc ttcttcggtg atgtcaaacc gcgccgccag caccggcaca tccagtaatg
    17581 ccagcacgtc ttccgacaca aagcggctgt ctggcaacga taacaggctg ataaacgctt
    17641 ccagtaccgg atgcgactgc cgcgcccgac ggtcggaaat ggcgtaaggc aggtaacgat
    17701 ccgcaggtgc gctaccaaac acagcctgaa taaacggact gtagctgtcg atatcagcca
    17761 ccatcacgat gatgtcgcgc ggagtaagtg tcgggtcttc ttccagcatc gccagcaggc
    17821 gatcgtgcaa aacttcaact tcacgctgcg ggctatggca aacgtggaag gtgatactgc
    17881 tatccagtgg atcaagcggg cgtttgttat cgctacggga aaactcttcg atgttcacac
    17941 cagcaacggc acggttttcc agttccagaa tgtcagactg aatgttatgc agcaggttat
    18001 ctggcgtgac atcgacaaag gcgtccagct cctggctgct ctccaggtca gaaaggagat
    18061 aaatgtagtc gcgcccaagc ttcccccatg aggccagcag cgggttgccg acatcctgtt
    18121 caccatcgct gttaaagagc tgtccggcat tttcgctatc gcgaaatagc ggcaattcgc
    18181 gatcttcaaa actgtgtcga cgctgacgag tcagcagttt cgccagataa gcaggatctt
    18241 taatatcgcc ccagtaataa cggcaggggt tggtaaacag gagatggatt tcaatatgtt
    18301 tacccagcgc ctggagcgcc tggagataaa caggcggtaa cgcggaaata ccgcatataa
    18361 agacgcgcga aggtaacccc ggcgggcagg tcgtcgcgga ctccagcgtt tcgataaagc
    18421 gctgatagag attcgcgcgg tgccagcgcg gttgtccgag ttcatgggta tattccacca
    18481 gcgccttcca caacggcgct tgccaggcct gtgcttctcc cagcccttca accaaatgtc
    18541 ctgtttccca ttgtgccagc cactccgggc gatagaccag atactggtca aacaggtccg
    18601 ccgcttttga ggaaagctgg aacagttttc gcttgtcgct atcgtcagtc agataatgcc
    18661 gcaacagggt aaagtcttcg tgctccaaca gttgcggcag cagagtcatc agtttccagc
    18721 tcatgctctg tttgttaaag gcgctctctt tggggatctc cggtaacacc cggacgaaca
    18781 tatcccagat aaagctcgca ggcagcggaa aatcaatgtt tgccgcaata ccaaactttt
    18841 gcgacagggt catttgcagc cactgtgcca taccggtact ttgcaccaga atcatctctg
    18901 gttcgaaagg atcgtccagc cgttcgcgtt cgacaataaa ctccatcaac gcttccagca
    18961 cgtccagacg attggaatgg tagaccctta acatagcggc tcctgactac tgacgattcg
    19021 ggcaatgcag gcgggtcatc tcgccttctc tgcccccggg tgaaactagc gtaacgctga
    19081 tgctgacaca tcccgcctgc gatgtctgca atcggttgac ctgccagtta gctggtggcg
    19141 aaatcgcgcg cagttgcgtt tgctgccagc catgccgcca gagctgttgg tactggtttc
    19201 tgctggcaag actgttcatc aatgttcgct ggaaacccga taacgcagtg acaatcatca
    19261 ccatcaacac catcgccaac attacctccg gcaggctaaa gccttgttga ttcttcaggg
    19321 aagctgacat aacgccctct ctttcagcgg acaaaaatcg ctccagccgc ttggcgaaaa
    19381 aacaatgttt ccatcaatga cttcgcctgt tcgccacaac gaaacgcctt cataaccggc
    19441 aatcaataag gcttcattat ctgccagtaa acgcaaacaa acctgggcat cggtttcagc
    19501 gtactgcgag cactgaactg ctggctgcgt ctgccaggag tgcatttttc cccacgccag
    19561 cgccgactga acgatggcct ggcggcgcaa tgactgactt tccatgctca cgcgagaggc
    19621 aaaactgcga tcctgttggc tcattccttg taatagcaaa ctacccaaaa ccagcagcat
    19681 caggaccaga gccagtgacg aaacaccctt ttcgcggttc acaggttgaa tcctgtcacg
    19741 ctatagctgg cattcaccac ggtttgcggt tcagacttac tggcagcacg catattaacc
    19801 gtcaacaccg gcgagaagcc gctgacatcc tgacgtacga cctgaaaagt gtcgataatg
    19861 atggcatccg gattagtgac tttatcccag cccttacctt cacaggatgt cgcaccgcgt
    19921 agcgtttcca gcacatgctc cttcagacga aatccaatct ggtcggactc ttttaccggt
    19981 tcgcgatccc agataccgtt actgttcgca tcccactgca caatgataca gtcaccctgt
    20041 ccgacaattt ccagcccttc gccggtacag atgccatgac aataacccgc cctctggaga
    20101 tgcttcgcga cggtaaatac ccgcagccag atttcatctt ccagcgccag cttacgggtg
    20161 ctcgttaaac tttcacgctg taacgcaggc agaaagcgtg cagcccccag caacaatacg
    20221 ctactgatcg ccatagcaat caacacttcc agcagagaaa aaccttgctc ttttacaggc
    20281 atccttctgt ttctccttgc tgacaaagcc ggagtcttcc ccacggcgaa accaccagcc
    20341 accactcgcc cgttgagttt ttgaagcgaa tatgcccggc ccatgcggta ttgcgcaggc
    20401 caaagaaagc aagcgaaggt gtcaggtcgc tcatttcgac ttcgggccag cgtggcacaa
    20461 agaccaatgg tgaactgcca tgacaggtat tggccccagc agcggaactc acaaggcacc
    20521 ataacgtccc ctccctgata acgctgatac tgtggtcgcg gttatgccag ttggcatctt
    20581 cacgtaaata gagcaaatag tcccgcgcct ggctggcggt ttgccatagc cgttgcgact
    20641 gctgccagta ttgccagcca tagagtccac ttgcgcttag catgaccaaa atcagcatcg
    20701 cgaccagcgt ttcaatcagc gtataaccac gttgtgtttt catgccggca gtatggagcg
    20761 aggagaaaaa aagacgaggg ccagtttcta tttcttcggc gcatcttccg gactatttac
    20821 gccgttgcag gacgttgcaa aatttcggga aggtgtctcg aagaatttaa tggagggtaa
    20881 aaaaaaccga cgcacactag cgtcggctct ggcaggatgt ttcgtaatta gatagccacc
    20941 ggcgctttaa tgcccggatg cggagcgtag ccttcaatct caaaatcttc gaaacggtag
    21001 tcgaagatgg attcgggttt acgtttgata atcaacttcg gcagcggacg cggttcgcgg
    21061 cttaattgca gatgagtttg atccatatga ttgctgtaca ggtgcgtgtc gccccccgtc
    21121 cagacaaaat cgcccacttc cagatcacac tgctgcgcca tcatatgcac cagtaacgcg
    21181 tagctggcaa tgttaaacgg caggccgagg aaaacgtcac aggagcgctg ataaagctgg
    21241 caagagagtt tgccgtctgc cacatagaac tggaagaacg catggcacgg tgccagcgcc
    21301 attttatcca gttcgcctac gttccacgct gaaacaataa tgcggcgcga atccgggtcg
    21361 tttttcagct ggttcagtac cgtagtgatc tggtcaatat gacgaccatc cggcgttggc
    21421 caggcgcgcc actgtttacc atacactggc ccgaggtcgc cgttttcatc ggcccattcg
    21481 tcccagatgg tgacattgtt ttcgtgtaga taagcaatgt tagtgtcgcc ctgcagaaac
    21541 cacagcagtt catggatgat ggaacgcagg tggcaacgtt tagttgtcac cagcgggaat
    21601 ccatcctgca ggttaaaacg catctgatga ccaaaaatgg aaagcgttcc ggttccggta
    21661 cggtcgtttt tctgtgtgcc ttcgtcgagc actttttgca tcagttctaa atactgtttc
    21721 atggttcctc aggaaacgtg ttgctgtggg ctgcgacgat atgcccagac catcatgatc
    21781 acacccgcga caatcatcgg gatggaaaga atttgtccca tgctgatgta ctgcacccag
    21841 gcaccagtaa actgcgcgtc gggctggcgg aaaaactcaa caatgatgcg aaacgcgccg
    21901 taaccaatca ggaacaaacc tgagacagct cccattgggc gcggtttacg aatatacagg
    21961 ttgaggataa taaacagcac tacaccttcc agcaacagct cgtaaagctg tgatgggtgg
    22021 cgcggcagca caccgtaagt atcgaaaatg gattgccact gcgggttggt ttgcagcagc
    22081 aaaatatctt ctgtacggga gccagggaac agcatggcaa acgggaagtt cgggtcaacg
    22141 cggccccaca attcaccgtt aataaagttg cccagacgcc cggcaccaag accaaacgga
    22201 atgagtggtg cgataaaatc agagacctgg aagaaggaac gtttagtacg gcgggcgaag
    22261 ataatcatca ccacgataac gccaatcagg ccgccgtgga aggacatgcc gccgtcccag
    22321 acacggaaca gatacagcgg atcggccata aactgcggga agttgtagaa cagaacataa
    22381 ccaatacgtc caccgaggaa gacgccgagg aagcccgcat agagtaagtt ttcaacttca
    22441 tttttggtcc agccactgcc cggacgattc gcccgacgtg ttgccagcca cattgcaaaa
    22501 atgaaaccca ccagatacat caggccgtac cagtgaagcg ccacgggtcc tattgagaaa
    22561 atgaccggat caaactccgg aaaatgcaga tagctactgg tcatctgtca ccacaagttc
    22621 ttgttatttc gctgaaagag aacagcgatt gaaatgcgcg ccgcaggttt caggcgctcc
    22681 aaaggtgcga ataatagcac aaggggacct ggctggttgc cggataccgt taaaagatat
    22741 gtatatgatc cgcgctataa ccctccgcga atcagcccgc ccatgccgcg acgctccata
    22801 aaggctacaa cctgatgacg aacttcggtc gccagttgcg cttccagact acgctgcgca
    22861 agattttctg cttcggcaaa atcaatgcgc cgcagcaggt attttacccg cgctacagaa
    22921 cgtccgttca tagacagatg gcgataccca agcccaatga ggattgccac gcacatggga
    22981 tcgcccgcca tttcaccgca caaacggaga tcgataccat gtatttccgc ttcccgggcg
    23041 atcattgcca gagctcgtaa cattgcagga tgaaggctgt cataaatgtt cgccacccgg
    23101 gtattgttgc gatcaacggc cagaatgtat tgagtcagat cgttggtgcc aacagagatg
    23161 aaatcgaccc gctttgccag atgcggcagc ataaatacca ttgacggtac ttccagcatg
    23221 atgccgatac gtggtttggg aatttcgtaa ccgatcatct cctcgacttc acgtccggca
    23281 cgttcaatca ggcggcgtgc ttcgtcaact tcatcgaggc tggtgaccat cggcaacaga
    23341 atattcaggt tgcccgtagc ggcattagca cgcagcatcg cccgcacctg gatcaagaag
    23401 atctccggct gatcgagcgt aatgcgaatc ccacgccaac ccaggcatgg attctcttcg
    23461 ctgatcggca tgtaaggcag ctgcttatct gctccgacat ccagcgtacg caaggtgacg
    23521 ggtttatcat tgaacatttg cagcatcccc tgatactgcg ccacctgttc ttcttccgac
    23581 gggaaaccac tttgcagcat gaatgggatt tcagtgcgat aaagaccgat gccatcaata
    23641 cggctgccca gtttttcttc atgttccggg cttaaaccag cattgagcat gacttttata
    23701 cgctcaccgc tttttaactg cgcgggtaaa ttgacgtcat cttccgccag acggctaagc
    23761 tcaatctctt cactaattag ccgctgatat tcttgcagca gtatcggctc cggatcgacc
    23821 agcaattcac cgcgatagcc atcaacgatc agcgtcctac gatgcagcac cgaaggctga
    23881 atatccgcgc ccatcacggt agggagcccc agcgcacgta ccatgatcgc agcatgggag
    23941 ttggctgcgc catctcgcac gacaacaccg actaagcgat cctggggcag ctcagcaaga
    24001 gttgtcgctg acagttcatc tgccaccaga atgaaacgtt ccggccaggc gttcggccct
    24061 tgattagcgt catcaagatg aaacagcaat cgctgaccca gcgcacgtaa atcgccagcc
    24121 cgctctttga gatagttatc gcttagcgcg gcaaactgtt cggcaaattt ttcaatgacc
    24181 gtttttaccg cccactctgc caccgagcct ttatcaacct cggcaaacaa ttcgcgacgc
    24241 agccgggtgt ccgaaagcag gtgcgagtaa agatcgaaaa tagccgccgt ttctttttgt
    24301 gctccggcgg caaagcgttt gctgtagcgg cgaaactcgt ttgccgcttc ttccagcgcc
    24361 ccggtcaatc gttcgcgttc cagagccgga tccagcgttg atgcctgata cacctgttcc
    24421 attaaaggta acgtggcatc ctgccagcct tcggcaatcg ccacaccagg tgctgccggt
    24481 aatgcgcgga ttcgcgtctg gcgatattgc ccaaacaagg cagtcaactg cgactgagaa
    24541 agaatagctg ccatctgggt ggcaagcgtc accaggaagg attcttcact ttcgtcatac
    24601 tggcgcaact ctcgttgctg taccaccagt acaccaagca actggcgacg ttgaataatt
    24661 ggtacgccta aaaacgcgcg gaaacgttct tcttttacgg aggggatgta tttgaagctg
    24721 gggtgctttt gcgcatctgc aaggtttatc ggttccgcca gcctgccaac caggccgacg
    24781 atcccttcat caaacgcgag cgttacagtg cgaccgcgtg gttttttcag cccccgggtc
    24841 gccatcaggt agtaacaacg tcgatcatga tcggccaggt agaccgaaca gacctcggta
    24901 tccatcgcaa gacagatgtc ggtaaccaga atatttaacg cctcattcag gcgtggtgcg
    24961 ctggctacct tttcgactat ttcgcgcagg cgagtgagca taattggcgt gacttaacct
    25021 cttttacgtc gataagcaga tgtgttttgt ggttttggcg tattttcctg cagtgacatc
    25081 accacactcg cgaactcttt cattacccta cggtagacat cacgtttaaa tgacaccacc
    25141 tgtctgaccg gataccagta acttacccat cgccagccgt caaactctgg tgtactgctg
    25201 gtttgcatat tgatttctgc atcgccgctc accagctgca agagaaacca tttttgtttt
    25261 tggccgatac aaaccggctt cgtgtcccaa cgcaccaaac gtttcggtaa tttgtagcgc
    25321 aaccagttac gcgttgaagc aaggattcga acgtctttgc ggcttaatcc tacttcttca
    25381 aacaattcac ggtacatcgc ctgctctgcg gattctccgg ggttgattcc gccttgcgga
    25441 aattgccagg agtgctgacc aaatcgccgg gcccacatta cctgcccctg gcgattacaa
    25501 atcacgatac ctacgtttgg gcggtagcca tcgtcatcaa tcaccggact acctcaaaat
    25561 aaagctttat atacgaatga ttgtttcata ctccaggaag acggtaaacc actctctgca
    25621 gggcattaca cactaataac aattgaataa ctcacagtta tgtgcagagt tataaacaga
    25681 ggaaggggtg gatagccgtt tttattcacc ttttctgtgg atagagttgt gaagaactac
    25741 ggaattacta cgggaaaacc cggagcattc tgaataagcc cgatataggc attttaaaat
    25801 attcattata agtcaatgca ttacatgtaa tttgagtaat gaaaaagagt aattcgtgac
    25861 ccaggtcaca cctctcattt acgggttggc gaaagatcaa ccaatgccgt atttatccac
    25921 agaatgtgcc actaagttaa gcactgaacc actaaaaact ggagtttcgt cgcacgtcaa
    25981 ggctgtaaat ggaaacagta gtggaggttt ttcacagtta tcccagcttt ctgtggataa
    26041 catggtgtaa gatcctgttt attttcagtg accagatttg gaaaacctat tgcagtgctg
    26101 cgcaactgga ttatcggcag cctaaaaagc aatataaatc agagaattga acaacacatg
    26161 tggaaaaagt tacactgcga atattcggta cataattgct gtttgttttt taaccaaggt
    26221 atcatgacat gtcccaacct cgcccactgc tctctcctcc cgaaactgaa gaacagttgt
    26281 tagcgcaagc acagcaactt tctggttata cattgggaga actggcggca cttgccgggc
    26341 tggttacgcc agagaattta aaacgcgata agggctggat tggcgtgtta ctggagatct
    26401 ggctaggtgc cagcgcaggg agtaaacctg agcaagattt tgctgctctg ggcgtggaac
    26461 ttaaaactat ccctgtggat agtcttggtc gtccgctgga aacaacattc gtttgtgttg
    26521 ccccgttaac gggtaatagc ggggtgacct gggaaaccag ccacgtgcgc cacaagctca
    26581 aacgcgtact gtggataccg gttgaaggcg agcgcagcat cccgctggcg cagcgtcgcg
    26641 taggatcccc gttgctgtgg accccgaatg aagaggaaga ccgacagcta cgcgaagact
    26701 gggaagaatt aatggatatg attgttctcg gtcaggttga gcggatcacc gctcgtcacg
    26761 gggaatattt acagataaga ccgaaagcag cgaatgcgaa agcgcttacc gaagccattg
    26821 gtgcccgggg cgaacggatt ctgacgctgc cacgcggctt ttatttgaag aagaatttca
    26881 ccagtgcact actggcccgt cattttctga tccagtagcc atcgctttga cctgccgctt
    26941 tccgggcata taattaccgc ttcatttttt tggcagggct ttttagatgt tatttgcatg
    27001 gataaccgat cctaacgcct ggcttgcgct cggtacgctg acgctgctgg agatcgttct
    27061 tgggatcgac aatattattt tcctttctct ggtggtggca aagcttccca cagcacaacg
    27121 tgctcatgcg cgccgtctgg ggttggcggg agccatggtt atgcgtctgg cgctgctggc
    27181 atcaatcgcc tgggttacgc gcctgacaaa tccgcttttt acaatattca gtcaggaaat
    27241 ttctgcccgt gatttgattc tgcttctggg tggcttgttc cttatctgga aagccagcaa
    27301 ggaaatccac gaatccattg aaggtgaaga agaagggctg aaaacacgcg tttcatcatt
    27361 cctcggcgct atcgtgcaga ttatgctgct ggatattatc tttagcctcg actcggtgat
    27421 tacggctgtg ggtctgtcag atcacctgtt tattatgatg gcagccgtgg tgattgccgt
    27481 aggcgtgatg atgttcgccg cgcgctcgat tggtgatttt gtcgaacgcc atccttcggt
    27541 aaaaatgctg gcgctctctt tcctgattct ggtgggcttt accctgattc tggaaagttt
    27601 cgacatccac gtaccgaaag gttacatcta cttcgcgatg ttcttctcta ttgcggttga
    27661 aagcctcaac ttgattcgca acaaaaagaa tccgctctga tactccgtac gctctcctgc
    27721 gggagggcgt actcactcct tttaccctca cagtttaaga ttttcctgct ttcaagatat
    27781 ataacgtcgt tttataaaca gactattatc ataggtaggc caaatgagga taaccggatg
    27841 aaaaaatggg cagtaataat ttctgcagtc ggactggcgt ttgctgtttc cgggtgttcc
    27901 agtgattacg tcatggcgac caaagatggc cgtatgattt tgaccgatgg aaaacctgaa
    27961 attgatgatg ataccgggct ggtgagttat cacgatcagc aaggtaacgc gatgcaaatt
    28021 aaccgtgatg atgtttcgca aattattgaa cgttaacaaa taaggtcagc atccggctgg
    28081 ccttaagatt tttctctttc ccttttccct tccctctgcc atttttatat tccttatgtc
    28141 gtgattataa aaaggaaacg gctatgcaat atcaccgtat accccacagt tcgctggaag
    28201 tcagcacgct ggggcttggc acgatgacgt ttggtgaaca gaacagcgaa gccgacgccc
    28261 acgcacaact cgactatgcc gtcgctcagg gcattaacct tatcgacgtt gccgaaatgt
    28321 acccagtacc tccgcgcccc gaaacgcaag ggttaaccga aacctacgtc ggcaactggc
    28381 tggcgaaaca tggcagccgc gaaaagttaa ttatcgcctc caaagtgagc ggaccgtcgc
    28441 gcaataatga caagggcatc cgcccggatc aggcgctgga tcggaagaat atccgcgaag
    28501 cgctgcatga cagcctcaag cgcctacaga ctgattacct caatctttat caggtgcact
    28561 ggccgcagcg cccgaccaac tgcttcggca aactcggtta tagctggacg gattctgcgc
    28621 ctgcggtttc gctgctggat acgctggacg cactggcaga gtaccaacgc gcgggaaaaa
    28681 ttcgttatat cggcgtgtcg aacgaaactg catttggcgg aatgcgctac ctgcatctgg
    28741 cggacaaaca cgatctgccg agtattgtca ccattcagac cccttacagt ctgttaaacc
    28801 gcagttttga agtaggtctg gcagaagtca gccagtatga aggggtcgaa ctgctggcct
    28861 attcatgcct gggtttcggc acgctgaccg ggaaatatct caatggtgca aaaaccgctg
    28921 gcgcacgtaa tacgctcttt agtcggttca cccgctatag cggtgagcaa acgcaaaaag
    28981 ccgtcgcggc gtatgttgat atcgccagac gtcatggcct ggaccctgct cagatggcgc
    29041 tcgcgtttgt acgccgtcaa ccgtttgttg ccagcactct gctgggcgca accacgatgg
    29101 atcagctgaa aactaacatc gaaagtttgc atctggagtt aagcgaagac gtattagctg
    29161 aaattgaagc ggtgcatcag gtttatactt atccggcacc ataagattaa cgccggttaa
    29221 aaccggcgtt aaatattaat gacggcgctg ccagatccac agcgccgtta ttgccagcgc
    29281 aaacagcgca ccgaagccaa tgccaatggg cacgaccggg atgcctacca ttaccgccag
    29341 cgagtaaatg cccagcatca acaacatggc gctgttttcg ccaaggtttt gtactgcaat
    29401 cgcattcccc gccccgacgc tttttttacc ccgctcctgt agcaacgcat tgagcggaac
    29461 gacaaaaaag ccccccatca cgccaatcag catcaacaag gcataggctg gcagcagctc
    29521 gtgttgcagg gaaaaaatca gtaccaccac gccaatcaaa atcccggctg gcatacagcg
    29581 tgacacggtt tccagcgtaa ctaacttcgc tgccgcacct gcgccaacca cgataccaat
    29641 cgctaccatc gcgttgagat aggtgggcgt agcgttatcg gtaatgccca gcgccaccgg
    29701 tacccacagc accaacagga aacgcagcgt gacacccgct ccccagaata aactggtgcc
    29761 caccagcgaa aaacgcgttt caccattgcg ccatagcgag gtgcaggcat tcaggaaact
    29821 gcgggtcatg ttgatgagat tccaagactg ccccggacgc gccgccgcca gtttgggaat
    29881 gtagatattg gcaacgaccg caccaccgta ggccagtgcg catgcggcca gggcgacgag
    29941 gacatgccag tcagccagca caccaccggc tacggaaccg agcaaaatcg ccgctatggt
    30001 agaagcttcc attaaaccgt tagctttcac taacttacta cccgtggtta attcgccgag
    30061 aataccgtat ttcgccggtg aataggctgc agcaccaacc cccaccagcg tatagccgag
    30121 aaacggattg ataccaaagc agatactggc tgcgcccagc agcttcaggc cgttggcaaa
    30181 catcatcacc cggcctttgg cgaagctatc cgccacctgc ccgacaaacg gcgcaaaaag
    30241 aatgtaagca cctacaaaca ccatttgcag gatgggctgg ctccactccg gatagaactg
    30301 cgctttcagt aacgccagag tggcaaacag tagggcatta tcgccaaacg cagagagaaa
    30361 ctgcgccacg ataaccgctt tcatcccctt cgaccacaac gaagtgttag tgtgcactga
    30421 ctcactcatc gtgttgttcc gcttcgtcta cccagctttt caacgtgaca aagtcaggtt
    30481 tgccgctgcc aagtaatggc atctgtttca gatagcgaat atcgcgcggt acagcaagct
    30541 ccggcacgcc gtgctcgcgg gcatactgtt gcaacttatc gagcgtcagt tcgttatctg
    30601 tggtgaaaag caccagtgcc tcgcctttgc tggcatcgct cttaatcgca gtggcatgga
    30661 ctttatctgg cgaaacacca agtgccagtt gttccaccat ttccagcgac accatttcgc
    30721 ctgcaatttt ggcaaagcgt tttgcgcggc cctgaatctg cacaaagccc tgctcgtcaa
    30781 aacgcacaat atcgccagtg tcataccagc cgcgctccat ttcgccgcga acattctcgg
    30841 cggtgggcac ttccagtaca cctggcttct ccacccgcag atagccgttc attatgttcg
    30901 gccctttcag ttgcaggcgt ccgccctctt cgataccagg aaccgacaac aggcgcgcat
    30961 ccattcctgg tagaatacgc cctaccgtac cgggtttcgc cgccatcggt acgttgatag
    31021 aaacgacagg cgcgcactcg gttacaccat agccttcaag gatgcgcagg ccaaatttat
    31081 cctgccaaag ctgtttggta ctttcttgta gtttttctgc gcctgccacc acatagcgta
    31141 gacgatagaa gtcatacggg ttggcgaaac gcgcgtagtg accgaggaaa gtcgaggtac
    31201 cgaacaacac ggtgcaactg cggtcataca ccaactccgg cacaatgcgg taatgcagcg
    31261 ggcttggata aaggaacact tctgcacctg taagcagtgg cgtaaacagg cctaccgtca
    31321 gcccaaagga gtgaaacagc ggtaatgccg acataaagcg atcgttggtg gtgaagtcgg
    31381 caatcgtttt aatctgctcg acgttcgcca gaatgctttt atggctatgg acgacgcctt
    31441 ttggatggcc ttcagaaccg gaggtaaaga ggatcagcgc ctcttcttcc ggctgctgtt
    31501 ttacctgcgc caggcgcggc atcagcaaat gagcgaagat ccatactttg tcggcagtgg
    31561 tgacatctgc ttttaaatct tccagataga cccagcgcac ctgagtaagt tgctccggca
    31621 gatgccagag tttgccttta tcgagaaact ggcgggaagt gaagatggtt ttgatttcag
    31681 ccgccgtaat agcactggtc agccctttta ccccagcagt gtagttcatc attgcgggaa
    31741 tgcggcgacg cgcgatggcc ccaaaaatca ctgccgcact gatgcctgca ttgggcagca
    31801 ttaagccgat gcgttcgcct tcaacactgt atttttcaag gatgcgtcca acaaacagcg
    31861 ttttcgtaag caatttgcga taggagtctg gggtaaagtt gacgtcttcg acacatttct
    31921 tcccggctcc gaagcggtac attgcactca gtaaagattc gtacagagtt tcacgcgggc
    31981 gcaccgccat acgcgcttcc atcattattt gatgcagcat ttcgccagcg attttgcgac
    32041 ggtcacgggc acgcgacgca tccggcatcg ccacctgcgt tggaggcaaa atatgcagag
    32101 taatttgcgg gaacaagcga cgtttaacca gacctttcag gcggctgaag tgcgtaagtt
    32161 ccgccccttc aatacgcaca ggaataaccg ttgcaccaga cttcgccgcg acaaaacccg
    32221 cgccatcgta gattttcatc agcgagcctg tagtggtgat gcgtccttca gggaaaatca
    32281 ccactggtcg gccctgttcc accagacgta ccagatgttt aatagccata ggttgcgtcg
    32341 ggtcgagagg aacaaagtcg ataaatgatt tcagccaacg catataccac tgttggctta
    32401 ttgaggtgta aacggcaaac actggacgca caggtaaaaa cagtccaagc aaaatgccat
    32461 caataaaaga gacgtgatta ggcgtaatta gaacgcgctc gcccttcagt gcctgggtgt
    32521 cacccgtaac gcgaacgcga tacaaaacac ggcacaaatt tcgaaaaaag ctaaaaagca
    32581 ttccaactcc ctttgctctg attcagtaaa agcgaatgga gggagattac acgagataaa
    32641 gaacgcgagc gacagtaaat taggtgcgaa aaaaaacctg cgcatccgcg caggttggtg
    32701 caagagacag ggtacgaaga gcgtaccgaa taatctcacc aatcaatacc tctgggatca
    32761 ccactttagc aacctgaagc caaacgccac cagcggtcaa tcgcaacagc gtttcgcaaa
    32821 gtgtaaccaa aggtttgcaa tctctttttt tgtcttactt atttgcgttt ggctcacatt
    32881 cccacgatga aaacacgcca ctccttgaac caacgggcgt tttccgtaac actgaaagaa
    32941 tgtaagcgtt tacccactaa ggtattttca tggcgaccat aaaggatgta gcccgactgg
    33001 caggcgtttc agtcgccacc gtttcccgcg tcattaataa ttcacccaaa gccagcgaag
    33061 cttcccggct ggctgtgcat agtgcaatgg agtctcttag ctatcacccg aacgccaacg
    33121 cccgtgcgct ggcgcagcag accactgaaa cggtcggtct gatcgttggt gatgtttccg
    33181 atccgttttt cggtgcaatg gtgaaagcgg tcgaacaggt ggcttatcac accggtaatt
    33241 tcttattgat tggcaacggt taccacaacg aacaaaaaga gcgtcaggct attgagcaac
    33301 tgatccgcca tcgctgtgct gcgttggtcg tccatgccaa aatgatcccg gatgccgatt
    33361 tagcctcatt aatgaaacaa atgcccggta tggtgctgat caaccgtatc ctgcctggct
    33421 ttgaaaaccg ttgtattgct cttgacgatc gttacggtgc ctggctggca acgcgtcatt
    33481 taattcagca aggtcatacc cgcattggtt atctgtgctc taaccactct atttctgacg
    33541 ccgaagatcg tctgcaaggg tattacgatg cccttgctga aagtggtatt gcggccaatg
    33601 accggctggt gacatttggc gaaccagacg aaagcggcgg cgaacaggca atgaccgagc
    33661 ttttgggacg aggaagaaat ttcactgcgg tagcctgtta taacgattca atggcggcgg
    33721 gtgcgatggg cgttctcaat gataatggta ttgatgtacc gggtgagatt tcgttaattg
    33781 gctttgatga tgtgctggtg tcacgctatg tgcgtccgcg cctgaccacc gtgcgttacc
    33841 caatcgtgac gatggcgacc caggctgccg aactggcttt ggcgctggcg gataatcgcc
    33901 ctctcccgga aatcactaat gtctttagtc cgacgctggt acgtcgtcat tcagtgtcaa
    33961 ctccgtcgct ggaggcaagt catcatgcaa ccagcgacta accgcagtta aagcaattcc
    34021 agcgccagta attcttcgat ggtctggcga cggcgaatca accgcgcctg accattatca
    34081 aacagaactt ctggtaacag cgaacggcta ttgtagttgg atgacattga tgcgccatat
    34141 gcccctgtat catgcagtac cagataatca cctgccttca cttccggcaa ggcgcgggtt
    34201 tcaacatttc ccccttcctg ctgggtaaag acatcgcccg attcacataa cggtccggcg
    34261 acgacggttt ccaccgttgg cgcgtgttcc agagaacgac catcagctgc cagggcactg
    34321 atatggtggt aactaccgta cattgccggg cgcatcagat cgttgaaccc ggcatcaacc
    34381 agcacaaagt ggcggctccc catttgtttg acgctccgca cctgagtaat taatacgcca
    34441 gactgcgcta ccaggaagcg acccggttca atttccagtt tcacagggtg gcccaaatgg
    34501 cgggcgattt gctcacgcgc ggcattccac agaccataat aatgttcggt atcaaccgcc
    34561 tcttcaccct gttgataagg aacagaaagc ccaccgcccg cagaaatagc ctgtaaatcc
    34621 tgaccgaatt cgatgacctg acgcaccata gcaccacaca cctgttccag atgggcataa
    34681 tcaacgccag aaccaatgtg catgtgaatg ccgaccagct gcagatgatg acgttgtatc
    34741 acgtccagtg cggcgggcag atcggtgtac cagataccgt gcttgctgtt ttcgccaccg
    34801 gtattggttt tttggctatg tccgtgacca aaccccggat taacgcgcag ccatacccga
    34861 tgccctggcg aaacctggcc cagttggtcg agcatatcaa cagaacccgc attcaccgga
    34921 atttgcaatt cactgacgcg ttcaagcgtc gcctgatcga taacatctgc cgtaaaaaca
    34981 atatcatcgg ggtgcgtttg cggattgtaa cccgccgcca acgcacgctc tatttcgcct
    35041 aacgagacgg aatccacttt cacgccctgc tcacgcatta agcgcaaaat atgaatattg
    35101 gaacaggctt tctgtgcaaa gcgcaccaca tcaaactgtt tcagcgctgc aatctgccga
    35161 cgaataattt gcgcatcgta gacccacacc gggcagccaa attcagcggg caaacgcagc
    35221 agattttcgg cggtgagatc ggtatcggtg ctgaacagtg aatgtggcat aacaaactcc
    35281 agataagtgc ttttttatga ttacgccaca tcataaaaag aataaaaaat atcgatttat
    35341 gtcgagtcta tgcaaaaatg atatggatta ccggattgcg agagagcgct aatggccgcc
    35401 gttaacttac gtcatattga aatttttcat gcggtaatga ccgccggaag cctgactgag
    35461 gcggcacacc tgctacacac ctcacagcca accgtcagcc gcgaacttgc gcgctttgag
    35521 aaggtgatcg ggctgaaatt gtttgagcgc gtacgtgggc gattacatcc taccgtgcaa
    35581 ggactgcgtc tgtttgaaga agtgcaacga tcctggtacg gactggatcg aattgtcagc
    35641 gctgcagaaa gtctgcgcga gtttcgccag ggagaactgt ctattgcctg cctgccggtc
    35701 ttttcgcaat cttttttacc gcagctcctg caaccctttc tggcacgtta tcccgatgtc
    35761 agcttaaata tcgtgcccca ggaatcaccg ctacttgaag agtggctctc ggcccagcgt
    35821 catgatttag gactcactga gacactccat acgcctgcgg gaacagaacg taccgaatta
    35881 ctctctttag atgaagtgtg tgtgttacct ccgggtcatc cgctggcggt aaaaaaggta
    35941 ttaacgccgg atgattttca gggtgagaac tacatcagcc tttcccgtac tgacagctat
    36001 cgccagttgc tggatcagct atttactgaa catcaggtta aacgacgcat gatcgtagaa
    36061 acccacagcg ccgcgtcaat ctgcgcaatg gtacgggcgg gggtaggtgt ttcggtggtt
    36121 aacccgctca ccgcactgga ttatgcggca agcggtttag tggtgcggcg gttcagtatt
    36181 gcggttccgt tcacagtcag cctgatccgc cccctgcacc gcccgtcatc agcgctggtg
    36241 caggcgttta gtgagcattt acaagcgggg ttaccgaaac tggtcacttc tcttgacgct
    36301 attttgtcgt cagctacgac agcataaaag cgacagcatc ctcggcatgg atcgccgcag
    36361 tatcaaacac aggcaaaaca ctgcgctctt ctggcaccag taaaccaatt tctgtgcagc
    36421 caaaaatgac gccctgtgcg ccctgttctg caaggcgagc aatcacttgc gcataataag
    36481 cgcgtgacgc ttcggtaaat tgccccagac acagttcttc aaaaataatc tgattaattt
    36541 tcgcccgttc atccgcttca ggaataagac aattgatgga aaattgttcc gtcagccgcc
    36601 cgcgataaaa atcctgttcc atggtgtaac gcgtacccag cagcgccaca cgagtcattc
    36661 cggccccggt aattgcacgt ccggtggcat ccgcaatgtg taagaaaggc agagtgcaac
    36721 gtgactcaat ggcatccgcc actttatgca tcgtattggt acataacaca ataccttctg
    36781 cgcccgcccg ctgtaagcca agcgccgcct cagccagaat gtccccggtt ttatcccatt
    36841 ccccgcgacg ctggcactct tctatttcat gaaaatcgac gctatgtagc agcacttgcg
    36901 cagagtgaag cccaccaagc cgctgtttaa tgccttcatt tatcaaacga tagtaaggaa
    36961 tggtggattc ccagctcatt cctcccagca aaccaattgt tttcattcct ctctcctgat
    37021 gtgtgttacc gcaacaaggc tgaataagga acgagacaaa cgcctcaacg gccaagtgcc
    37081 caatctctat taacgaaaaa agggccggat gtacagcaca tccggcccgt gaaatcagat
    37141 gccgatattt ctcaacttct cgcctgccat cagtttgcgt tcgatatgtt ccagcgtgac
    37201 atttttggtt tccggaatga gccagaaagt gatgcccaca aacgcaatgt tcagcgcagt
    37261 gtagagccag aacgtaccgg cagcgccaat gctgtcgagc agcgtcagga aggtcgccga
    37321 taatcatatt cgacacccag ttcgtggtgg tcgaacaggt aataccgaaa tcgcggcatt
    37381 tcagcggctg aatttcagag cacaggatcc acaccactgg cgcggcgctc atcgcataac
    37441 cggcaataca catcatcgtc atgccaacag agagccagga caatccactg gaagccgtac
    37501 cgttatcaaa ctgcatcagg caatagccca gcaccagagt gcctaacgcc atcacgctga
    37561 aaccaatttt cagagccggt ttacccctgc tttatctacc gtaaacaccg caataaaggt
    37621 ggcgaacata aaggtcagcc ctacgaccag agtcgcaatc atctgttgtt ctgtggtcgt
    37681 aaagcccgcc attttgaaga tacgcggcgc gtagtacatg atgatgttca taccggtaaa
    37741 ctgctgcatc gcctgcaaca acataccgag aaacacagca cgacggacgt tacggttgat
    37801 cttaaacagt gcccaaccgc cctgttttaa cttcaggctt tcacgaattt cgttgagttc
    37861 ttctcgcgct ttttccgacg tatcgcgcag catacgcaat acttcttccg cctcaatatg
    37921 acgccccttt tccgccaacc agcgcgggct atttggcagg aagaccacca gaataatcag
    37981 cagaactgct ggtaaagcaa gaacccccaa cattgcgcgc cagttaccgc tataactgaa
    38041 cgctgtatcg gataaaaacg ccagcacgat gccgagtgtg accatcaact ggtacatact
    38101 gatcatctta ccgcgaacgt tttcacttgc catttcagaa agatacagag gagcggtgta
    38161 agacgcgatc ccgacagcaa tgcccagccc cacacgagcg gcgattaaca tctctacgct
    38221 ggtcgcaaaa gcggacccta tagaaccgag tacaaacagg atggcccccg ccatcaggct
    38281 gtatttacgc cccaggcgga acgacagcca accattaaac agcgcaccaa ttgctgcgcc
    38341 gagcatcatg ctactaacca cccattcctg caaatgactg gtcagcacaa agtgatcggt
    38401 aatgaacggc aacgctccgg cgattacgcc gatatcaaga ccaaataaca atcctgcgac
    38461 cgcagcagct accgaaacaa acatattcat acgccgcgta tcccgcaaag aacgtggcgt
    38521 taaagcagat tccgtattga tagtaaccat tttttcctgc cagcagagag taagacatag
    38581 tgaaaaaata cgtgaacaac tcacgcaggt gtcaggtcgg aaacagcata aatatggatt
    38641 aaattgctgc gacatgtcgt tatgtgatgg atattccaat tttcaaatta agttgaatta
    38701 ttgagattat tattaaccac ctaattttac agcagataaa attcataaag ttcattaatt
    38761 gataattaat atggattatt tcataaccat gatatggatt atgatgatct acaggtataa
    38821 aaaaccctgc catgcggcag ggtcataaaa gtaagaagaa tgaattaacg cgccagccaa
    38881 ccgccatcca cggcaatggt ataaccattc acataatctg aagcgctgga ggcaaggaac
    38941 actatcggcc ccatcaggtc actcggcagt ccccaacgac cagctggaat gcggtcgaga
    39001 atttccgcgc tacgttgttc atctgcccgt agttgttgag tattgttggt cgccatgtaa
    39061 cccggagcta tcgcattaac attaatgttg tgtttagccc attcgttcgc catcaatcgc
    39121 gtcacaccca tcacgccgct ttttgatgcg gtataagaag gcacacggat cccgccctgg
    39181 aaggagagca ttgacgcgat attgataatc ttgccgccat tgccttgcgc gataaagtgt
    39241 ttcgccgctg cctgagacat gaagaatacg ctcttgatat tcaggttcat gacatcgtcc
    39301 cagtcttttt cgctgaactc gagagcgtct tcgcggcgaa tcaatccggc gttattcacc
    39361 aggatatcaa tatgaccaaa ttcagctacc gcgcgatcca gcagtccagg aatgccatca
    39421 atctttcgca gatcggcggt caggcttaaa aaacgacgcc ccagcgccgt gacctgctcg
    39481 atggtttcag tcggttcaac gatgttaatg ccaacaatgt cacagcccgc ttgcgccagc
    39541 cccaacgcca tcccctggcc cagcccagta tcacaaccag tgacgaccgc aactttacct
    39601 tcgagagaaa atgcacttaa aatcataaca ataccttatt cgtttatgcc cacaactagc
    39661 gcaaatcttt aacggccaca tggtccatat catcaaagac ctggttttca ccaaccatgc
    39721 cccagataaa ggtataagct ttggttccga cgccggaatg gatcgaccag ctcggggaga
    39781 tcaccgcctg ctcgttatgc atcacaatgt gacgcgtttc ttgcggttgc cccatcatgt
    39841 ggaaaacgca ggcgtcatca tccatattga aatagaaata aacttccatc cggcgctcgt
    39901 gggtgtgaca cggcatggtg ttccacaagt tacctggagc cagctccgtc agccccatac
    39961 tcaattggca ggtttccagc acatccggga caaaatattt gttaatagtg cgacggttac
    40021 tggtgaggtt atcgcctaac gtgactggag atacttcgtc cggggtgact tttttggtgg
    40081 gatacgtcgt atgcgcgggt gcgcaattgt aataaaactt tgccggagtg gcggtatcaa
    40141 tactggcaaa gacaacttct tttgcacctt taccaacata cagggcgtcg cggtgaccga
    40201 tttcatagca ttggccatcg acagtaatcg taccggcacc gccaatattg ataacaccta
    40261 actcgcgacg ttcaaggaaa tagcttacgc cgagttgttt accaacttcc ccgccaacgg
    40321 aaaccgtttt agttatcggc ataatgccgc caacaataat tcggtcaatg tggctgtaaa
    40381 ccatggtgta ctcatcggcg acaaatacct tttcaaccaa aaattcattg tgcagccctt
    40441 gggtatccag cgtttttgcg tgcgcactgt ggatgctctg tcttacgtcc acatcaacct
    40501 ccgataatca gtgaagcgtg aaaatagaaa acgatagtga aacactgttt cgttttctct
    40561 ataaagggat gatatgccca tgggctaggg ttttcaatta aaaataaaac aatgttttat
    40621 ttttaagtta aggattaaaa aaagtgcagt gtcgatcacg aatatgtcat gagaatggaa
    40681 aaatgaggga gcccagaaaa tgtatgtcga cttgccagcc atcattattc aacagattaa
    40741 tgacggcaga tatttatcgg taaggaggaa atgcagagct actcgtcacg ttcaatggtc
    40801 aatgccacgc cctggccccc gccgatacaa agcgttgcca gccctttgcg ggcattacgt
    40861 ttcaccattt catgaaccag agaaaccagg attcggcaac cggaagcgcc tatcgggtga
    40921 ccgagtgcga tcgcgccacc attgacattg acccgacgct catcccactc aagcatcttg
    40981 ccaaccgaaa gcgcctgtgc agcaaacgct tcattagcct cgataagatc gacatcagcc
    41041 aactgccagc ctacacgctc caggcaacgg cgggtcgcat acaccggcgc aattcccatc
    41101 aatgccggat ctacaccaac gctggcaaat gcgcggatgc gggccagcac gggtaaattc
    41161 aacgctcgtg ctttggcttc gctcatcatc attaccgcag ctgcgccatc gtttatggat
    41221 gatgcattcc ccgcggtcac agaaccgaga ctatcaaatg aaggatttaa acgggctaaa
    41281 ccttctgcac tggcgtcagt gcatggctgt tcatcggtat caacaaccaa cgtctgaccg
    41341 ttactttgga tcattaccgg gacgatctca tctttaaatc gtccggcgtc aatcgccgct
    41401 cgcgcttttt gttgcgagct aagtgcgtaa gcatcctgca actgacggct gatgccatat
    41461 tcgcgagcca gattttcggc ggtgacacca atatgataat cattgaaggc atcccacaac
    41521 ccatcatgca ccagactgtc aaccaactgg ctattgccaa gctgtgcacc ggtgcggcta
    41581 tcagtcagaa catgtggtgc gcggctcatg ttttcctggc caccggcgat gacaatatca
    41641 gcctcgccac actgtatcgc ctgagtagcc agatgcagtg ctttaagccc agaaccgcaa
    41701 acgtcattaa tagtgattgc agaaacgcta ttaggaagac cacctttaat agccgattgc
    41761 cttgccggat tctgccctgc ccctgcagtc aacacctgac caagaattac ttcatccacc
    41821 gcatatgcag gaacgccggt acgttctatt aacgctttca cgaccaaact accaagttcc
    41881 acggcggaat gacccgctaa cgcaccacga aagcagccga taggtgtccg taacgccccg
    41941 acaatcacaa cgtctttcat cacaacctcg caccaattaa cagtgcaata gtaaatgatt
    42001 gttaattaca gttatcttaa ttgtttaaaa aaagtgattt ttatcacaaa agaaatatgc
    42061 ctgagcagca gtcagagaca taactggcac gtaaggtttg caaccactaa cccaccaata
    42121 gaggggtaga tagggcgtta atctcccata cttaacctgg tttatggtaa attgccctcc
    42181 attttgttta atttgtagat gatacgttca gataatgtct aatatttggt ctaaagaaga
    42241 aactctgtgg agtttcgcgc tctacggcac agccgttggt gcaggcacgc tcttccttcc
    42301 tattcagtta ggttcggcag gggctgtggt cctgtttatt actgctctgg tcgcctggcc
    42361 tttaacatat tggccacata aagctttatg ccagttcatc ctctcatcga aaacatcagc
    42421 aggtgaaggg ataacgggcg cggtaacaca ctactatggc aagaagattg gtaatctgat
    42481 taccacgctg tacttcatcg ccttttttgt cgtcgtgttg atatatgcag tggcaattac
    42541 caactcactt acagaacagc tggcaaagca tatggttatt gatcttcgca tccgtatgtt
    42601 ggtgagtctg ggt
//
LOCUS       DAJTMC010000010        42217 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.010, whole genome shotgun sequence.
ACCESSION   DAJTMC010000010 DAJTMC010000000
VERSION     DAJTMC010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 42217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42217
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.010"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(1..607)
                     /gene="yeeE"
                     /locus_tag="OO130_000509"
                     /pseudo
     CDS             complement(1..607)
                     /gene="yeeE"
                     /locus_tag="OO130_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416517.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transport protein YeeE"
     gene            complement(786..2144)
                     /gene="plaP"
                     /locus_tag="OO130_000510"
     CDS             complement(786..2144)
                     /gene="plaP"
                     /locus_tag="OO130_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HCR5364035.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYRVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(2134..2196)
                     /gene="yoeI"
                     /locus_tag="OO130_000511"
     CDS             complement(2134..2196)
                     /gene="yoeI"
                     /locus_tag="OO130_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HCR5364036.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(2411..3340)
                     /locus_tag="OO130_000512"
     CDS             complement(2411..3340)
                     /locus_tag="OO130_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5364037.1"
                     /translation="MKPLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLALEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQHFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(3386..4210)
                     /locus_tag="OO130_000513"
     CDS             complement(3386..4210)
                     /locus_tag="OO130_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR5364038.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGSGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGMVKETTPRNPVTNSGLVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(4293..4547)
                     /gene="yoeB"
                     /locus_tag="OO130_000514"
                     /pseudo
     CDS             complement(4293..4547)
                     /gene="yoeB"
                     /locus_tag="OO130_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
     gene            complement(4544..4795)
                     /gene="yefM"
                     /locus_tag="OO130_000515"
     CDS             complement(4544..4795)
                     /gene="yefM"
                     /locus_tag="OO130_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HCR5364039.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDIIE"
     gene            5078..5128
                     /gene="hisL"
                     /locus_tag="OO130_000516"
     CDS             5078..5128
                     /gene="hisL"
                     /locus_tag="OO130_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HCR5364040.1"
                     /translation="MTRVQFKHHHHHHHPD"
     misc_feature    5104..5227
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            5274..6173
                     /gene="hisG"
                     /locus_tag="OO130_000517"
     CDS             5274..6173
                     /gene="hisG"
                     /locus_tag="OO130_000517"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HCR5364041.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            6179..7483
                     /gene="hisD"
                     /locus_tag="OO130_000518"
     CDS             6179..7483
                     /gene="hisD"
                     /locus_tag="OO130_000518"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HCR5364042.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     ARGDEALREYSAKFDKTTVTALKVSAEEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPAAD
                     MARRVAEAVERQLAELPRAETARQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARDLVDGITSAGSVFLGDWSLESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            7480..8550
                     /gene="hisC"
                     /locus_tag="OO130_000519"
     CDS             7480..8550
                     /gene="hisC"
                     /locus_tag="OO130_000519"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HCR5364043.1"
                     /translation="MSIVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYGVSAETIGVECRTVPTLDNWQLDLQGISDKLEGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVAQIIAER
                     ECLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            8550..9617
                     /gene="hisB"
                     /locus_tag="OO130_000520"
     CDS             8550..9617
                     /gene="hisB"
                     /locus_tag="OO130_000520"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HCR5364044.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAIDRANSYVIGDRATDIQLAENMGITGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            9617..10207
                     /gene="hisH"
                     /locus_tag="OO130_000521"
     CDS             9617..10207
                     /gene="hisH"
                     /locus_tag="OO130_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HCR5364045.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            10207..10944
                     /gene="hisA"
                     /locus_tag="OO130_000522"
     CDS             10207..10944
                     /gene="hisA"
                     /locus_tag="OO130_000522"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HCR5364046.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYATQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPEMVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            10926..11702
                     /gene="hisF"
                     /locus_tag="OO130_000523"
     CDS             10926..11702
                     /gene="hisF"
                     /locus_tag="OO130_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HCR5364047.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVQEVQKRGAGEVVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            11696..12307
                     /locus_tag="OO130_000524"
     CDS             11696..12307
                     /locus_tag="OO130_000524"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HCR5364048.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVAFFSRTKQRLWTKGETSGNFLNVVSIAPDCDNDTLLLLVNPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(12404..13381)
                     /gene="wzzB"
                     /locus_tag="OO130_000525"
     CDS             complement(12404..13381)
                     /gene="wzzB"
                     /locus_tag="OO130_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HCR5364049.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIVTQPDVGQIAGYTNAMNVIYGSAVPKVSDIQASLIGRYSTA
                     FSALAETLDNQEEAEKLTIEPTVKNQSLPLAVSYVGETPEGAQKQLAKYIQQVDDQVN
                     EELGKDLKDNIALRMKNLQDSLKTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIDNLDVDKLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(13528..13620)
                     /locus_tag="OO130_000526"
                     /pseudo
     CDS             complement(13528..13620)
                     /locus_tag="OO130_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
     gene            13790..14794
                     /locus_tag="OO130_000527"
     CDS             13790..14794
                     /locus_tag="OO130_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010432020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase"
                     /protein_id="HCR5364050.1"
                     /translation="MKFLVTGAAGFIGFYLSKRLLAAGHQVVGIDNLNDYYDVNLKQA
                     RLDLLKHDNFSFYKIDLADREKMAALFADERFERVINLAAQAGVRYSLENPNAYADAN
                     LIGFLNILEGCRHNNVQHLLYASSSSVYGMNRKMPFSTDDSVDHPVSLYAATKKANEL
                     MAHTYSHLYGLPTTGLRFFTVYGPWGRPDMALFKFTKAMLEGKSIDVYNFGKMKRDFT
                     YIDDIAEAIIRLQDVIPEKNPQWAVETGSPATSSAPYRVYNIGNSSPVELMDYINALE
                     EALGIEANKNMMPLQPGDVLETSADTKALYDVIGFKPETSVKEGVKNFVEWYRNFYKV
                     "
     gene            complement(14860..16026)
                     /gene="ugd"
                     /locus_tag="OO130_000528"
     CDS             complement(14860..16026)
                     /gene="ugd"
                     /locus_tag="OO130_000528"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HCR5364051.1"
                     /translation="MKITISGTGYVGLSNGILIAQNHEVVALDIVQAKVDMLNKRQSP
                     LVDKEIEEYLATKDLNFRATTDKYDAYKNADYVIIATPTDYDPKTNYFNTSSVEAVIR
                     DVTEINPNAVMIIKSTIPVGFTESIKERFGIENVIFSPEFLREGKALYDNLHPSRIVI
                     GEQSERAKRFAALLQEGAIKQDIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNSRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADSILARKPKVVGVYRLIMKNGSDNFRASSIQGIMKRIKAKGVPVIV
                     YEPAMKEDDFFRSRVVRDLDAFKQEADVIISNRMSADLADVADKVYTRDLFGND"
     gene            complement(16275..17681)
                     /gene="gndA"
                     /locus_tag="OO130_000529"
     CDS             complement(16275..17681)
                     /gene="gndA"
                     /locus_tag="OO130_000529"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HCR5364052.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIT
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFHDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELIAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLTNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEEGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKEQRVAASKVLSGPQAQPAGDNAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(17786..18619)
                     /locus_tag="OO130_000530"
     CDS             complement(17786..18619)
                     /locus_tag="OO130_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001016535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCR5364053.1"
                     /translation="MNHRLEKFSVLISIYKNDLPQFFEVALRSIFHDQTLKPDQIVIV
                     ADGELHQTHIDIINSFIDDVGNKIVTFVPLPRNVGLANALNEGLKACRNELVARMDAD
                     DISLPHRFEKQISFMINNSEIDVCGSFIDEIETVTEEFISTRKVPLEHREIVKFARKR
                     SAVSHPSVIFRKNTVLAVGGYPPFRKSQDFALWSLLIVHNARFANLPDILLKMRTGRN
                     LMARRGLSYLLYEYKVLYYQYKIGFIRKNELISNAMLRTFFRIMPSKLKELMYSIVRN
                     R"
     gene            complement(18616..19485)
                     /locus_tag="OO130_000531"
     CDS             complement(18616..19485)
                     /locus_tag="OO130_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000381997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCR5364054.1"
                     /translation="MDTVYAVLVAYNPEHNDLKNAVELLLRQVTKVVVCNNSTNGYKY
                     AENSSGDVKIFNFNDNLGIAEAQSIGMKWAFENGADFILQMDQDSIPDPKMVEQLLTC
                     YKKLLKQNVNVGLVGSQDFDKVTGELNKARVKKGKPLTEVYYEVDSTLSSGSLIPKNS
                     WLIVGGMKDELFIDAVDHEYCWRLRAAGFKVIRNKNALLAHRLGDGRFKILNILSVGL
                     PSPFRHYYATRNIFLLLNKNYVPIYWKISSLVKLIGKVFLYPIFLPNGNKRLYFFLKG
                     INDGLMGRSGKMK"
     gene            complement(19502..20689)
                     /gene="wzy"
                     /locus_tag="OO130_000532"
     CDS             complement(19502..20689)
                     /gene="wzy"
                     /locus_tag="OO130_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O147 family O-antigen polymerase"
                     /protein_id="HCR5364055.1"
                     /translation="MTSDFYNSKDKSLSVLLFFGFIFFLTRSFPFIQYSWIMEGFLCL
                     CIMSFTKKIANGIYHYPVILIFLLALFINFIYSYIKGNDIAIIIRFYIIILFILCAYF
                     CSYGTISIVKIFLYLMVLQAVIISIISIYMTKTYGIGDYSALRHYFLENDYGDVYTYG
                     SGFYRVQIKGNALIPFAFMLHIVIKDYFYYRFKNTITVILAIGTIVAGNFAYFVSICL
                     FFMYIILCSKSNSRYAKLRKIIFGVFLTVILPFFITYSIELIIMKSNGADSSLGVRWD
                     QFTVLINDLTESVSNFVIGSGLGNVIKIQTPIRDYSAYIYYELQSVYFLNQLGVILFT
                     LFLLINLLLTIKIIKYSELCVLYFLYVSYAITNPYILDSNHVAVIIVLVTLSNVLKKM
                     KAK"
     gene            complement(20745..21977)
                     /gene="wzx"
                     /locus_tag="OO130_000533"
     CDS             complement(20745..21977)
                     /gene="wzx"
                     /locus_tag="OO130_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O147 family O-antigen flippase"
                     /protein_id="HCR5364056.1"
                     /translation="MSIVKNTLWNISGYIIPSLIAIPALGILSRILGTEQFGLFTLAI
                     ALVGYASIFDAGLTRAVIREVSIYKNVHKELRAIISTSTVILTILGLIGGSVLFLSSN
                     VIVKLLNINANHVVESVKAIYIISATIPLYLLNQVWLGIFEGMEKFRKVNLIKSINNS
                     FVAGLPVIFCFFHGGLLSAIYGLVMARVLSLIVTFIFSRKLIISSGLSVKIVTVKRLI
                     GFGSWITVSNIISPIMTYMDRFILSHIVGADKVSFYTAPSEGIQRLTILPSALSRAIF
                     PRLSSELQSVKQTKILSYFIMVIGILPIVMLIIILSDFIMSAWMGPTYHGTPGIVLKI
                     LAIGFFFNCIAQIPFVSVQASGRSKITAIIHLLEVIPYLCILYIFIYHWGIVGAAIAW
                     SVRTSLDFLILLLIDTKY"
     gene            complement(21974..22531)
                     /gene="rfbC"
                     /locus_tag="OO130_000534"
     CDS             complement(21974..22531)
                     /gene="rfbC"
                     /locus_tag="OO130_000534"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HCR5364057.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFMESFNQKVFEEAVGRKVE
                     FVQDNHSKSSKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFLVLSETAEFLYKTTNYYHPDSDRGIVWNDPILSIKWPTI
                     EHNNYILSIKDARAKELHNMKELFL"
     gene            complement(22536..23414)
                     /gene="rfbA"
                     /locus_tag="OO130_000535"
     CDS             complement(22536..23414)
                     /gene="rfbA"
                     /locus_tag="OO130_000535"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCR5364058.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPTPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDVAVNKESGATVFAYHVNDPERYGVVEFDKNGTAISL
                     EEKPLQPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMEQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAYRKGFIDAEQVKALAEPL
                     KKNAYGQYLLKMIKGY"
     gene            complement(23472..24371)
                     /gene="rfbD"
                     /locus_tag="OO130_000536"
     CDS             complement(23472..24371)
                     /gene="rfbD"
                     /locus_tag="OO130_000536"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HCR5364059.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKRIRPDVIVNAAAHTAVDKAESEPEFAQLLNATSVESIAKEANEVGAWVIHY
                     STDYVFPGNGDTPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAAKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVALNKPEVAGLYHLVAS
                     GTTTWHDYAALVFEEARKAGIPLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELITTTAI"
     gene            complement(24371..25456)
                     /gene="rfbB"
                     /locus_tag="OO130_000537"
     CDS             complement(24371..25456)
                     /gene="rfbB"
                     /locus_tag="OO130_000537"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCR5364060.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYVFEHADICDAAAMARIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALDSDKKTRFRFHHISTDEVYGDLPHPDEVNNTEELPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDAEKISRELGWKPQETFESGIRKTLEWY
                     LSNTKWVDNVKSGAYQSWIEQNYEGRQ"
     gene            complement(25829..26722)
                     /gene="galF"
                     /locus_tag="OO130_000538"
     CDS             complement(25829..26722)
                     /gene="galF"
                     /locus_tag="OO130_000538"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HCR5364061.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            complement(26954..27949)
                     /locus_tag="OO130_000539"
     CDS             complement(26954..27949)
                     /locus_tag="OO130_000539"
                     /EC_number="5.1.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl-diphospho-ditrans,
                     octacis-undecaprenol 4-epimerase"
                     /protein_id="HCR5364062.1"
                     /translation="MNNNVLLIGASGFVGTRLLETAIADFNIKNLDKRQSHFYPEITQ
                     IGDVRDQQALDQALAGFDTVVLLAAEHRDDVSPTSLYYDVNVQGTRNVLAAMEKNGVK
                     NIIFTSSVAVYGLNKHNPDENHPHDPFNHYGKSKWQAEEVLREWYNKAPTERSLTIIR
                     PTVIFGERNRGNVYNLLKQIAGGKFMMVGAGTNYKSMAYVGNIVEFIKYKLKNVAAGY
                     EVYNYVDKPDLNMNQLVAEVEQSLNKKIPSMHLPYPLGMLGGYCFDILSKITGKKYAV
                     SSVRVKKFCATTQFDATKVHSSGFVAPYTLSQGLDRTLQYEFVHAKKDDITFVSE"
     gene            complement(28107..29501)
                     /gene="wcaM"
                     /locus_tag="OO130_000540"
     CDS             complement(28107..29501)
                     /gene="wcaM"
                     /locus_tag="OO130_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HCR5364063.1"
                     /translation="MPFKTLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGHTVVVPAGLVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGEEGGSMHNITLDVRGSDCTIKGLAMSGFGPVTQIYIGGKNKRVMSNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIK
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLVYKLRGIQISAGNAVSFVALTNIEMKRASLELHNKPQHLFMRNIKV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAMNERGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            complement(29512..30732)
                     /gene="wcaL"
                     /locus_tag="OO130_000541"
     CDS             complement(29512..30732)
                     /gene="wcaL"
                     /locus_tag="OO130_000541"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HCR5364064.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEILALQKGDTQN
                     THAAWTKYNLAARTHWLQDEPTGKIAKLRHRASQTLRGIYRKNTWQALNLKRYGAESR
                     NLILSAICGQVATPLHADVFIAHFGPAGVTAAKLRELGVIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGPWERRLRTLIEQYQ
                     LEDVVEMPGFKPSHEVKAMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLGTDELAPVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            complement(30729..32009)
                     /gene="wcaK"
                     /locus_tag="OO130_000542"
     CDS             complement(30729..32009)
                     /gene="wcaK"
                     /locus_tag="OO130_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HCR5364065.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPQAEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMKQHNSAAGVVGRVKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRSNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMMALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPALNARLSEAVSRERQTGMQMVQSVLERIGEVK"
     gene            complement(32285..33763)
                     /gene="wzxC"
                     /locus_tag="OO130_000543"
     CDS             complement(32285..33763)
                     /gene="wzxC"
                     /locus_tag="OO130_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HCR5364066.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCLAVFLLSDLI
                     GDVLNNPDLAPLIKTLSLAFVVIPQGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYRPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRINFYKPLSVVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQFLCVVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            complement(33765..35105)
                     /gene="wcaJ"
                     /locus_tag="OO130_000544"
     CDS             complement(33765..35105)
                     /gene="wcaJ"
                     /locus_tag="OO130_000544"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HCR5364067.1"
                     /translation="MVQRFSDITIMFAGLWLVCEVSGLSFLYMHLLVALITLVVFQML
                     GGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFNNDFDTQLKIWLAWYGLTS
                     IGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLMESFRNQPWLGFEVVGVYH
                     DPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGARVKKLVHQLADTTCSVLL
                     IPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLLKRAEDIVLATLILLLISPVLC
                     CIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVMENDKVVTQATQNDPRVTKV
                     GNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYRQLIEGYMLRHKVKPCITG
                     WAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIVFLTVFKGFVNKAAY"
     gene            complement(35210..36580)
                     /gene="cpsG"
                     /locus_tag="OO130_000545"
                     /gene_synonym="manB"
     CDS             complement(35210..36580)
                     /gene="cpsG"
                     /locus_tag="OO130_000545"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HCR5364068.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTSVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSRDALAVDRTDGISMTFADWRFNLRSSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            complement(36773..38209)
                     /gene="cpsB"
                     /locus_tag="OO130_000546"
     CDS             complement(36773..38209)
                     /gene="cpsB"
                     /locus_tag="OO130_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HCR5364069.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPENDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSSVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            complement(38212..39442)
                     /gene="wcaI"
                     /locus_tag="OO130_000547"
                     /pseudo
     CDS             complement(38212..39442)
                     /gene="wcaI"
                     /locus_tag="OO130_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
     gene            complement(39439..39918)
                     /gene="gmm"
                     /locus_tag="OO130_000548"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             complement(39439..39918)
                     /gene="gmm"
                     /locus_tag="OO130_000548"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HCR5364070.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLPGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASDNVHANSRAYFLAEKRAGVPGL"
     gene            complement(39921..40886)
                     /gene="fcl"
                     /locus_tag="OO130_000549"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             complement(39921..40886)
                     /gene="fcl"
                     /locus_tag="OO130_000549"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HCR5364071.1"
                     /translation="MSKQRIFIAGHRGMVGSAIKRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQSTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            complement(40889..42010)
                     /gene="gmd"
                     /locus_tag="OO130_000550"
     CDS             complement(40889..42010)
                     /gene="gmd"
                     /locus_tag="OO130_000550"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HCR5364072.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYCESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIVTGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLDDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            complement(42036..>42217)
                     /locus_tag="OO130_000551"
     CDS             complement(42036..>42217)
                     /locus_tag="OO130_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HCR5364073.1"
                     /translation="PIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKSLPVNVVC
                     RGNPAVVIRERVETE"
BASE COUNT        11057 a        10727 c         9744 g        10689 t
ORIGIN      
        1 tgacaaaggg atgccagcgt ttttcaaaca gaatatgagc gatcccggtt cggcgcggcg
       61 gtaaggtcgc gactttgagt tttggcttct tcaactcttt catcaccacc cagagcgtta
      121 tcaccagcaa aacggcaacc aacggccaca cagacaaatt aaaagtctca gcaatagagt
      181 tatgttcagt actgtagtgc tgcaaggttt gatttaaacc actggcatgt ggagaacgca
      241 tcaccgcact catcaccata taagtgaaaa gcgcgatcca actgccgatc aatccctcac
      301 ccgcgcgata ccaggtcccg gtggcacatc cacccgccag aacaattccc agcccgaaga
      361 tatacccacc tataacagta ccaggccacg ggaacgcccc ggcttcgtaa gtcagtaggc
      421 ccgcctaaat taacgcaaaa acccccacac tttgtaccga aattgcaatc agcaaggcgt
      481 aaaacatgcg attatttttc gcgatataca tatcgcgaaa accaccagtc aggcaaaaac
      541 gcccacgctg catgacaaat cccagcagag caccacaaat tagcccgctt aatatcattg
      601 aaaacataaa ttaattaacc agatgaatgt taatgaggaa attattcatg actggtggaa
      661 tgcagaccaa taaccaaatt ctctaaatta gaacaaatgg ttcttaatga ggatttcgat
      721 aaaaaagaat aaaaaaaccg gagccaaata ttcattcgac tccggttgat tattagaaaa
      781 gatgattatg ctacgtcttc gtactgcgga accgggttac ggaagctctt ggtgacgcaa
      841 gccaggtaaa tcagaccgat agcagcccag atcagaccca gaaccattga gctttcttcc
      901 aggttaaccc acagcgcacc aacggtcagc gcaccacaca tcggcaggaa cagatactgg
      961 aagtgatctt tcagcgtctt attacgcttc tcacggatcc agaactgcga gatgaccgac
     1021 aggttaacga aggtgaacgc caccagcgca ccaaagttaa tcagcgccgt tgccattacc
     1081 aggtcgaagt tgattgccag cagggcaatc gcgccaacca ggatgatgtt catcgccgga
     1141 gtacgccatt tcgggtgtac ataaccgaag aagcttttcg ggaatacgcc gtcacgaccc
     1201 attacgtaca tcagacgcgc tacgcctgca tgcgccgcca taccggacgc cagtacggta
     1261 atggtggaga agatcagcgc gccaacctgg aacgctttac ccgcaacgta cagcatgatt
     1321 tcaggctgtg acgcatccgg atctttaaag cgagagatat ccgggaagta cagctgcagg
     1381 aagtaagttg caaagatgaa gatcatgccg ccgatcagcg cggtcaggaa aatcgcacgc
     1441 gggatcacgc gctctgcatc tttggtttct tccgacaggt tgctgatgcc gtcaaagccg
     1501 gtaaaggaga agcacaggat tgtcgccccg gtaatcatcg ggataacatg cgcatcgcca
     1561 gaccagaatg gacgagtgct cgccagcgta ccagcgcctt caccttcaaa tacgcgataa
     1621 acaaccatgc ccagaatcac cgcgatcagc accacctgca acacgacgat tacagtgttg
     1681 aagttcgcta cggatttcag actacgcagg ttaaaggcgg tcatgaaggc caccaacgcc
     1741 accacaaaca tccacgatgg gatggaaggc accagagctt caaaatagat tttcgccagc
     1801 agaatgttga tcatcggcgc gaacagatag tcgagcagag aagaccaacc caccataaag
     1861 ccgacagtcg ggctaatgga tttctgggcg taagtgtatg cagagccagc agaaggatag
     1921 cggcgaacca gcttcccgta gctcagagcc gtaaacagga tcgcaatcaa tgcgaacgca
     1981 taggctgtcg gcacatgacc atccgtgagg cctgaaacga taccaaaagt atcaaacagc
     2041 gtcatcggct gcatataggc aagacccatc attacaaccg gaactaacgt aagcgtttta
     2101 cgcaattcca cgcgagaggt gtttggagta acgttatgcg acatggtcat tctcctttac
     2161 ggtgataacc gtcgcgtaag caaaaaattg ccccattttt ttggattcct cagcgacaac
     2221 aactgtcgat ttttagtaaa tatctatccg gtacgaagcc cggcctcttg gtatgaatga
     2281 ttggtttgaa gcaaaaaata accgacgctg atgaaacgtc ggtttttagt cattttttga
     2341 cagcggcgca ttgtgcctaa atggggggga aatgacaaga gaatgagagg cttgtcataa
     2401 taatttttct ttaaatggct gattttccgt catcagattt tgcataaaca ccggcgatcg
     2461 cactatttgc taaaatttca tcccgccacc agccactggc aagtcctgcg gtctgttcat
     2521 tccagccaat ccagacgggt tcaaagagag tttgggcgac cactttcttc tcgattaacg
     2581 cgccactatc gagaaaatgt tgcgccagat aacggggtaa atagccgcag ccgaggccgc
     2641 taatttgcag ctccagcttg gttttaaaat cgaaaacggt aatggcttct tgttcgtcga
     2701 gaagttgtga ggccgtagaa gcgcctgcct gcgcagtatc ccccaccaca atcgcccgat
     2761 atcgcttaat aatacgacga tttaacggct cttcttccag ggctaacgga tgatgaggcg
     2821 cgaccgcaaa gacttgttcc agatcgccca accgtgaaaa accaaactca ctggaagatg
     2881 gtggctcatg catcgcgcca acaataatat ccgctcgccc ctgagtcagg gcatcccatg
     2941 aaccacccag tacgccattg ataaacttca ggcgcgtcac gctatgatgt tgataaaaag
     3001 cttcaatcag tggcgctagc agagaaaaag gaaacgtgtc gtccacgcca atcaccagct
     3061 cgttttccca gccttcatgc agtttgatcg cctgcttttc cagttcccgc acggtatgca
     3121 gaacttcccg ccctttctct aataacattt ttccggtgcg ggtgaattta gcgcggtgac
     3181 cgctacgatc aagcaattga atattaaggt cgctttccag cttgtgaacg gtgtaactca
     3241 gagcggacgg cgtcttgtag agtttggccg acgccgccgc aaaactgcct tctttttcta
     3301 aggcatcgag gatcatcaac acatccagca gtggtttcat acacgccccc ttgcagtgta
     3361 tgcggcgtcc tgctggcgtg atcgtttact ccagcggcat taccagcgga tcgggatact
     3421 ggtattcaaa tcccagttca ttacaaatcc gactgccatc aataatctta cctttgccgc
     3481 tgtccagact atttctgaac tgcggaggtt ccagccccag taaacgggcc atctgcggat
     3541 agaaaacatt acgcgcaggg tgagctggcg cacatatatt atagatgtgt ccgcctttag
     3601 gtgcctgtaa caacagagtg atagcgccaa taacatcttc taaatggact aaattaacac
     3661 catgttcacc atcaggcgcg gtttttccgg caaagaagcg tccgggatga cgccccggtc
     3721 cgaccaggcc cgcaagacgc agaatatcga ccgaagtacc gggtaaattg tgcagccagt
     3781 cttcgagttc ttctaatact agtccactgt tggttaccgg attacgcggg gtagtttctt
     3841 tcaccatgcc ttgcgcatcg ccatagacag atgttgagct ggtaaaaata attcggggaa
     3901 tacgatgggc cagcgcgcta tccactaact cttgtaccgc ttgtaaatag aactcatcgc
     3961 cggagccgct acgacgtgcc ggaagcgtaa tgaccagtgc atcggcatcc atcagggcat
     4021 ccagatcgtc agaatcgcaa actaactcag gctccatgcg aagcagatag ctatcaatgc
     4081 cactcattcg ggccgcttcg acaccatctt gtgtggtttt actcccggtg acttgccagc
     4141 ctcgcgctga aagtgacatc gccagcggca tgcctaacca ccctaacccg acaattgcga
     4201 cctttttcat ccgttatctc ctgatacacc ttagatctat aaggctacgc tagcgtatca
     4261 aaactgacaa ttcattctat gaatgaatct gttcaataat gataacgaca tgctgcaatg
     4321 agcagtgaat cgtcggtaac cgcgtatacc agacggtgct cctctgtaat gcgtcgggac
     4381 tagaaacctg acaaattatg tttcaggggt tctggcttcc ccttaccttc aaatggcgtt
     4441 ctgcgggtat ctttgataag ttcattgatc tttttaacaa ttcgcttatc tgtttcctgc
     4501 cagtacagat aatcatccca tgattcctca gaccagatta gtttcactca ataatgtcct
     4561 tttctgttcc tttgcctgat ttcaggctat cgattgagtc catcaatctc cgggcgttag
     4621 cgggggaacg cagtagataa gccgtctctt ccagcgagtt gtattcttcg agtgacatca
     4681 gaacacaagc ctctccattc tgacgagtaa taaggatcgg ggcatgatct tcaacggctt
     4741 tcatcattgt tgccgacaaa ttctgacgcg cttcgctgta gctaattgta cgcatgtcaa
     4801 tctcctcttt tgtacagttc attgtacaat gatgggcgtt aattaactat ttattaatta
     4861 gtttgtagat caaggtattg tcagtgagac gaaaatccag gcttcgctat ttttggtgcc
     4921 gtcagcttag aggacagtcc tcttagcccc ctcctttccc cgctcattca ttaaacaaat
     4981 ccatcgccat agaatatata aaaaagccct tgctttctaa cgtgaaagtg gtttaggtta
     5041 aaagacatca gttgaataaa cattcacaga gacttttatg acacgcgttc aatttaaaca
     5101 ccaccatcat caccatcatc ctgactagtc tttcaggcga tgtgtgctgg aagacattca
     5161 gatcttccag tggtgcatga acgcatgaga aagcccccgg aagatcacct tccgggggct
     5221 tttttattgc gcggttgata acggttcaga caggtttaaa gaggaataag aaaatgacag
     5281 acaacactcg tttacgcata gctatgcaga aatccggccg tttaagtgat gactcacgcg
     5341 aattgctggc gcgctgtggc attaaaatca atcttcacac ccagcgcctg atcgcgatgg
     5401 cagaaaacat gccgattgat attctgcgcg tgcgtgacga cgacattccc ggtctggtaa
     5461 tggacggcgt ggttgacctc gggattatcg gcgaaaacgt gctggaagaa gagctgctta
     5521 accgccgcgc ccagggtgaa gatccacgct actttaccct gcgtcgtctg gatttcggcg
     5581 gctgccgact ttcactggca acgccggttg atgaagcctg ggatggcccg ctctccttaa
     5641 acggtaaacg tatcgccacc tcttatcctc acctgctcaa gcgttatctc gaccagaaag
     5701 gtatctcttt taaatcctgc ttactgaacg gttctgttga agtcgccccg cgtgccggac
     5761 tggcggatgc gatttgcgat ctggtttcca ccggtgccac gctggaagct aacggcctgc
     5821 gcgaagtcga agttatctat cgctcgaaag cctgcctgat acaacgcgat ggcgaaatgg
     5881 aagaatccaa acagcaactg atcgacaaac tgctgacccg tattcagggt gttatccagg
     5941 cgcgtgaatc aaaatacatc atgatgcacg caccgaccga acgtctggat gaagtcatcg
     6001 ccctgctgcc aggtgccgaa cgcccaacta ttctaccgct ggcgggtgat caacagcgcg
     6061 tagcgatgca catggtcagt agcgaaaccc tgttctggga aacgatggaa aaactgaaag
     6121 cgctgggtgc cagttcaatc ctggtcctgc cgattgagaa gatgatggag tgatcgccat
     6181 gagctttaac acaatcattg actggaatag ctgtactgcg gagcaacaac gccagctgtt
     6241 aatgcgcccg gcgatctccg cttctgaaag cattacccgc actgttaacg atattctcga
     6301 taacgtgaaa gcacgcggcg atgaggccct gcgggaatac agcgcgaagt ttgataaaac
     6361 cacggttacc gcactgaagg tgtctgctga ggaaattgcc gccgctagcg aacgcctgag
     6421 cgacgagcta aaacaggcga tggcggtggc agtgaagaat attgaaacct tccacactgc
     6481 gcaaaaactg ccgccggtag atgtagaaac gcagccaggc gtgcgttgcc agcaggtcac
     6541 gcgcccggtc gcttcagttg ggttgtatat tcctggcggc tccgccccgc tcttctcaac
     6601 ggtattaatg ctggcgactc cggcgcgtat tgcgggctgt aaaaaagtgg tgctgtgctc
     6661 accgccgccg attgccgatg agatccttta tgcggcgcag ctgtgcggtg tgcaggacgt
     6721 gtttaacgtc ggcggcgcac aggccattgc cgcactggcg tttggtacgg aatctgtgcc
     6781 gaaagtggac aaaatcttcg ggccgggcaa cgcctttgtc accgaagcga aacgtcaggt
     6841 gagccagcgt ctggacggtg cggcgatcga tatgcccgca ggcccgtcgg aagtgttggt
     6901 cattgccgac agtggcgcaa cgccggattt cgtggcttct gatttgcttt ctcaggctga
     6961 acacggcccg gactcacagg tgattttact gacgcccgct gctgatatgg cgcgtcgcgt
     7021 tgccgaggct gtcgaacgcc agctggcaga actgccgcgt gctgaaaccg cccgtcaggc
     7081 actgaacgcc agccgcctga tcgtgactaa agatttagcg cagtgcgtag agatctccaa
     7141 ccagtacggc ccggagcacc tgatcatcca gacccgcaac gcccgcgatc tggtcgatgg
     7201 cattaccagt gccggttcgg tatttcttgg tgactggtca ctggaatcgg caggtgatta
     7261 cgcctccggc accaaccacg ttctgccgac ttacggttac accgccacct gttccagcct
     7321 cgggctggca gatttccaga agcgcatgac cgtgcaggaa ctgtcgaaag aaggtttctc
     7381 cgcgctggct tcgaccattg aaacattggc cgccgccgag cgcctgaccg cccataaaaa
     7441 tgccgttact ttacgtgtta acgcccttaa ggagcaagca tgagcatcgt gactattacc
     7501 gatttagcgc gtgaaaacgt gcgcaacctg acgccgtatc agtcggcgcg tcgtctgggc
     7561 ggtaacggcg atgtctggct gaacgccaac gaatatccca cagccgtgga atttcagctt
     7621 actcagcaaa cgctcaaccg ctacccggaa tgtcagccga aagcggtgat tgaaaattac
     7681 gcgcaatatg caggcgtaaa accggaacag gtgctggtca gccgtggcgc ggacgaaggt
     7741 attgaactac tgattcgcgc tttttgcgaa ccgggtaaag acgccatcct ctactgcccg
     7801 ccaacgtacg gcatgtacgg cgtcagcgcc gaaaccattg gcgtcgagtg ccgcacagtg
     7861 ccgacgctgg acaactggca actggattta cagggcattt ccgacaagct ggaaggcgta
     7921 aaagtggtct atgtttgcag ccccaacaac ccgaccgggc aactgatcaa tccgcaggat
     7981 tttcgcactc tgctggagtt aacccgcggt aaagcgattg tggttgccga tgaagcctat
     8041 atcgagtttt gcccgcaggc atcgctggct ggctggctgg cggaatatcc gcacctggct
     8101 attttgcgca cactgtcgaa agcttttgct ctggcgggcc ttcgttgcgg atttacgctg
     8161 gcaaacgaag aagtcatcaa cctgctgatg aaagtgattg ccccctaccc gctctcgacg
     8221 ccggttgccg acattgcggc ccaggcgtta agcccgcagg ggatcgtcgc catgcgtgag
     8281 cgggtggcgc aaattattgc agaacgcgaa tgcctgattg ccgcactgaa agagatcccc
     8341 tgcgtggagc aggttttcga ctctgaaacc aactacattc tggcgcgctt taaagcctcc
     8401 agtgcagtgt ttaaatcttt gtgggatcag ggcattatct tacgtgatca gaataaacaa
     8461 ccctctttaa gcggctgcct gcgaattacc gtcggaaccc gtgaagaaag ccagcgcgtc
     8521 attgacgcct tacgtgcgga gcaagtttaa tgagtcagaa gtatcttttt atcgatcgcg
     8581 atggaaccct gattagcgaa ccgccgagtg attttcaggt ggatcgtttt gacaaactcg
     8641 cctttgaacc gggcgtgatc ccggaactgc tgaagctgca aaaagcgggc tacaaactgg
     8701 tgatgatcac taatcaggat ggtcttggaa cacaaagttt cccgcaggcg gattttgatg
     8761 gcccgcacaa cctgatgatg cagatcttca cctcgcaagg cgtgcagttt gatgaagtgc
     8821 tgatttgccc gcacctgccc gccgatgaat gcgactgccg taagccgaaa gtaaaactgg
     8881 tggagcgtta tctcgctgag caagcgatag atcgcgccaa cagttatgtg attggcgatc
     8941 gcgcgaccga tattcaacta gcagaaaaca tgggcattac tggtttacgc tacgaccgcg
     9001 aaaccctgaa ctggccgatg attggcgagc aacttaccaa acgagaccgt tacgcccatg
     9061 tagtgcgcaa caccaaagag acgcagattg acgttcaggt gtggctggat cgcgaaggtg
     9121 gcagcaagat taacaccggc gttggcttct ttgatcatat gctggatcag atcgccaccc
     9181 acggcggttt ccgcatggaa atcaacgtca aaggcgacct ctatatcgac gatcaccaca
     9241 ccgtcgaaga taccggcctg gcgctgggtg aagcgttaaa aattgccctc ggcgataaac
     9301 gcggtatttg tcgctttggt tttgtgctgc cgatggacga atgccttgcc cgctgcgcac
     9361 tggatatctc tggtcgcccg cacctggaat ataaagctga atttacctac cagcgcgtgg
     9421 gcgatctcag caccgagatg atcgagcact tcttccgttc gctctcttac accatgggcg
     9481 tgaccctgca cctgaaaacc aaaggtaaaa acgatcacca ccgtgtagag agcctgttca
     9541 aagcttttgg tcgcaccctg cgccaggcca tccgcgtgga aggcgatacc ctgccctcgt
     9601 cgaaaggagt gctgtaatga acgtggtgat ccttgatact ggctgcgcca acctgaactc
     9661 ggtgaagtct gccattgcgc gccacggtta tgaacccaaa gtcagccgtg acccggacgt
     9721 cgtgttgctg gccgataaac tatttttacc cggcgttggc accgcacaag cagcgatgga
     9781 tcaggtgcgt gagcgcgagc tgtttgatct catcaaagcc tgtacccaac cggtgctagg
     9841 catctgctta gggatgcaac tgctggggcg gcgcagcgaa gagagcaacg gcgtcgattt
     9901 gctgggcatc atcgacgaag acgtgccgaa aatgaccgac tttggtctac cgctgcccca
     9961 tatgggctgg aaccgcgttt acccgcaggc aggcaaccgc ctgtttcagg ggattgaaga
    10021 cggcgcgtac ttttactttg ttcacagcta cgcaatgcca gtcaatccgt ggaccatcgc
    10081 ccagtgtaat tacggcgaac cgttcaccgc ggcggtacaa aaagataact tctacggcgt
    10141 gcagttccac ccggagcgtt ctggtgccgc tggcgctaag ttgctgaaaa acttcctgga
    10201 gatgtgatga ttattccggc attagattta atcgacggca ccgtggtgcg tctccatcag
    10261 ggcgattacg gcaaacagcg cgattacggt aacgacccgc tgccgcgctt acaggattac
    10321 gccacgcagg gtgccgaagt gttgcatctg gtggatctga ccggggcaaa agatccagca
    10381 aaacgtcaaa tcccgttgat taaaaccctg gtcgcgggcg ttaacgttcc ggtgcaggtt
    10441 ggcggcggcg tgcgtagcga agaagacgtg gcggcgttac tggaagcggg cgttgcgcgc
    10501 gtggtggtcg gctccaccgc ggtgaaatca ccagaaatgg tgaaaggctg gtttgaacgt
    10561 ttcggtgccg atgccttagt gctggcgctg gatgtccgta ttgacgagca aggcaacaag
    10621 caggtggcag tcagcggctg gcaagagaac tcgggcgttt cactggaaca actggtggaa
    10681 acctatctgc ccgtcggcct gaaacatgtg ctgtgtaccg atatctcgcg cgacggcacg
    10741 ctggcaggtt ctaacgtctc tttatatgaa gaagtgtgcg ccagatatcc gcaggtggca
    10801 tttcagtcat ccggcggcat tggcgacatt aacgatgttg ctgccctgcg tggcactggc
    10861 gtgcgcggcg taatagttgg tcgggcatta ttggaaggta aattcaccgt gaaggaggcc
    10921 atcgcatgct ggcaaaacgc ataattccgt gtctcgacgt tcgtgatggt caggtggtga
    10981 aaggcgtaca gtttcgcaac catgaaatca ttggcgatat cgtgccgctg gcaaaacgct
    11041 acgccgaaga aggtgcagac gaactggtgt tctacgatat caccgcttcc agcgatggcc
    11101 gtgtggtaga taaaagctgg gtatctcgcg tggcggaagt gatcgacatt ccgttctgcg
    11161 tggcgggtgg gattaagtct ctggaagatg ccgcgaaaat tctttccttt ggcgcggata
    11221 aaatttccat caactcgcct gcgctggcgg atccgacgtt aattactcgc ctggcggatc
    11281 gttttggcgt gcagtgtatt gtggtgggta ttgatacctg gtacgacgct gaaaccggta
    11341 aatatcatgt gaatcaatat accggcgatg aaagccgcac ccgcgtcact cagtgggaaa
    11401 cgctcgactg ggtacaggaa gtgcaaaaac gcggtgccgg agaagtcgtc ctcaacatga
    11461 tgaatcagga cggcgtgcgt aacggttacg acctcgaaca actgaaaaaa gtgcgtgaag
    11521 tttgccacgt cccgctaatt gcctccggtg gcgcgggcac catggaacac ttcctcgaag
    11581 ccttccgtga tgccgacgtt gacggcgcgc tggcagcttc cgtattccac aaacaaataa
    11641 tcaatattgg tgaattaaaa gcgtacctgg caacacaggg cgtggagatc aggatatgtt
    11701 aacagaacaa caacgtcgcg aactggactg ggaaaaaacc gacggactta tgccggtgat
    11761 tgtgcaacac gcggtatccg gcgaagtgtt aatgctgggc tatatgaacc cggaagcctt
    11821 agacaaaacc atagaaagcg gcaaagtcgc cttcttctcg cgcactaaac agcgactgtg
    11881 gaccaaaggc gaaacgtcgg gcaatttcct caatgtggtg agtattgcac cggactgcga
    11941 caacgacacg ttactgttac tggtgaatcc tatcggacca acctgccata aaggcaccag
    12001 cagctgcttc ggcgacaccg ctcaccagtg gctgttcctg tatcaactgg aacaactgct
    12061 cgccgaacgc aaatctgccg acccggaaac ctcctacacc gccaaactgt atgccagcgg
    12121 caccaaacgc attgcgcaga aagtgggcga agaaggcgtg gaaaccgcac tggcagcgac
    12181 agtacatgac cgctttgagc tcaccaacga ggcgtctgat ttgatgtatc acctgctggt
    12241 gctgttgcag gatcaggatc tggacttaac gacggtgatt gagaacctgc gtaaacggca
    12301 tcagtgagtt gcggggtaag cggatgcgat attgttgtag catccggaaa aaaaaacggg
    12361 caaggtgtca ccaccctgcc ccttttcttt aaaaccgaaa agattacttc gcgttgtaat
    12421 tacgcagagc gttacgcccc aagacaatcc ccgcgccaac catgccgccc agcagcaccg
    12481 ccagaatcaa ggtaattgcc tttttcgggc tatcgcgacg aataggtaac gtcggtttca
    12541 ttacatagcg gtaagcatga atatcaagtt tatcaacgtc aagattgtcg atatccagca
    12601 ggttttgacg agtctgatag tagtttggtg agaacaccaa cggacgagtc gcttcatgct
    12661 taatcatcga ctccagcgct tcgctcccca aaaggaacaa cgtatcttgt gtcacatctt
    12721 cagtctgctg aacctgtggc tttgtcacct gcgcctgatt cgcatactgc aacgcttcct
    12781 gaatctgacg gatacgcaga tctttctgct cctgcgcaac tacttcctga gttttcagag
    12841 aatcctgcaa gtttttcata cgcagcgcga tgttgtcttt caggtctttt cccagttctt
    12901 catttacctg gtcgtcaact tgctggatat acttcgccag ctgcttctga gcaccttcag
    12961 gtgtttcacc aacataagac accgctaaag gtaatgactg atttttgacg gtaggctcaa
    13021 tggtcagctt ttctgcttct tcctggttat ccagcgtttc cgctaatgct gagaatgcag
    13081 tgctgtaacg tccgataagt gaagcctgaa tgtcggagac tttcggtaca gccgatccgt
    13141 agatcacatt catcgcgttg gtataaccag cgatttgccc cacatcaggt tgagtaacga
    13201 tagctgtgga agtccacttt tctttggcaa cggccagata ccctatagcc agggcaatag
    13261 caacaatgac agagatgata attgtcatct tgccacgcca caactgcacc agtaaatcaa
    13321 tcaaatcaat ctgttccgga tcatggtttt gcccagaaac attattattt tctactctca
    13381 tcattaccct aacggacgaa ataattttaa gtcgccatag tttagcgata atcgccatga
    13441 atgctatagg ataaatgata aaaataatga attattacaa agggaacata agagaatcag
    13501 ggcgaaaatc gccatgataa caggatgtta gtcactgcca aagagatcgc gggtgtagac
    13561 tttgtctgcc acatccttaa gctcttctgc catacggttg gagataatga cgtcggcttg
    13621 tatagtcagc ttagaacagc tttgattgta aaatgagcac gttaccagaa aaaacagcca
    13681 agtttagaac tgataccgat tatctttttt tcttgtctaa cggtataatt taactttcag
    13741 ttatgccaga tgaagattgg ctatattcta gcctgagcga ggattataaa tgaaatttct
    13801 ggttacggga gctgctggct ttatcggttt ctatctaagt aaacggcttc ttgcagctgg
    13861 tcatcaggtt gtaggcattg acaacttaaa tgattattac gatgtcaacc tcaaacaagc
    13921 acgacttgat ttactcaagc acgacaactt cagtttttat aaaattgacc tggccgatcg
    13981 tgagaaaatg gcggcactgt ttgcagacga gcggttcgaa cgcgtaataa acctcgctgc
    14041 gcaagccggt gtacgttact ctcttgaaaa tcccaatgca tatgcagatg caaacctgat
    14101 tggattcctg aacatactag aaggatgtcg ccataataat gttcagcatc tactttacgc
    14161 ttcctccagt tctgtttatg gcatgaaccg caagatgcct ttctctacag atgactctgt
    14221 agatcatcct gtttcgcttt atgcagcaac taaaaaagcg aatgaactca tggcccatac
    14281 atattctcat ttgtatggct taccgaccac agggctgcgt ttctttacgg tttatggtcc
    14341 atggggacgt ccggatatgg cattatttaa attcactaaa gccatgctgg aaggtaaaag
    14401 cattgatgtt tacaacttcg gcaaaatgaa gcgtgacttt acttacattg atgatattgc
    14461 cgaagctatt attcgcttac aggatgttat tccagaaaaa aacccacagt gggctgtaga
    14521 aacaggctcg cctgcaacaa gttcagcacc atatcgtgtc tataacattg gtaatagttc
    14581 gcctgtggag ttgatggact atatcaatgc gttagaagag gctctgggta ttgaagccaa
    14641 caaaaatatg atgcctctcc aacccggtga tgtactggaa accagtgctg atacaaaagc
    14701 actgtatgac gtaataggat tcaaacctga aacgtcagtt aaagaagggg taaagaactt
    14761 tgtagaatgg tatcgtaact tctataaagt ttgattttac aaaaccataa gaaaaggccc
    14821 taatttatta gggccttttc ttagaatgaa acaaaataat taatcattgc caaacaagtc
    14881 gcgcgtataa actttatctg ctacatcagc cagatcggca gacatacggt tagaaataat
    14941 aacatcagct tcttgtttga acgcatccag atcacgtacc acgcgcgacc ggaaaaaatc
    15001 gtcctctttc atagctggct cataaacgat tacaggcaca cctttcgcct tgattcgctt
    15061 cataataccc tgaatcgagg aagcacgaaa attgtctgaa ccattcttca taatcaaacg
    15121 atagacgcca acaactttcg gtttacgtgc aaggatagaa tcggcaataa aatctttgcg
    15181 cgtgcggttg gcgtcaacaa ttgccgagat caggttattc ggcacagact ggtaatttgc
    15241 cagtaactgc ttagtatctt tcggcagaca ataaccacca taaccgaatg acgggttgtt
    15301 gtagtgatta ccaatacgcg ggtcaaggca tacgccctca ataatctggc gtgaattaag
    15361 tcccaggctt tcagcataac tatcaagttc attgaaatac gctacacgca tcgccagata
    15421 agtgttcgca aaaagtttaa tcgcctcagc ctcggttgag tcagtaaaca atgttggtat
    15481 gtcttgctta atggcgcctt cctgtaataa cgcagcaaaa cgtttagcgc gttcagactg
    15541 ctcgccaatc acaatgcgtg atgggtgtaa gttatcataa agtgctttac cttcacgcaa
    15601 aaactcaggc gaaaagatca cattttcaat accaaaacgt tctttaatgg actctgtaaa
    15661 accaacaggg atagttgatt ttataatcat taccgcgttg ggattaattt ctgtcacatc
    15721 acgaatgacc gcttccacgc ttgaggtatt aaaataattt gttttcggat cataatcggt
    15781 aggtgtggca ataataacgt aatcggcatt tttatacgcg tcatacttat ctgtcgtagc
    15841 gcggaaattg agatctttag tcgccagata ctcttcaatc tccttatcaa caagcggtga
    15901 ctgcctcttg ttaagcatgt ccactttggc ctgaacgata tccagtgcaa ccacttcgtg
    15961 gttttgcgca atcagaatac catttgaaag accaacataa cctgttcctg aaattgttat
    16021 tttcattagc tctgacttct tccggttaaa catttagagt ggtcattaat ccaccaccac
    16081 agcttcatct actgcgggat ttttaacgct gatgtagatg aactgtcaag gcagcgatcc
    16141 tgctgtgcgg cgctgtatta tatcgcgttt ttaactataa attataaaaa aaggccctaa
    16201 cctgccgctt tgtataataa aaaagcccgg agggtttctc cgggccttgt tttgattaat
    16261 tgatttaaat cagattaatc cagccattcg gtatggaaca caccttcttt atcaatgcgc
    16321 ttataagtat gcgcaccgaa atagtcacgc tgtgcctgga tcaggttcgc aggcagaaca
    16381 gcggcgcggt agctgtcgta ataggcaacc gcagcggcga aggtcggcac cgggataccg
    16441 ttctgtactg cgtaagcgac gacatcgcgc agcgcctgct ggtagtcatc ggcaatttgc
    16501 ttgaagtaag gagccagcaa caggttagcg atctgcggat tttcggcata agcatcggtg
    16561 attttctgca ggaactgcgc acggatgatg cagccagcac ggaaaatctt cgcgatttca
    16621 ccgtagttca gatcccagtt gtactcttca gacgcagcgc gtagctgaga gaagccctga
    16681 gcgtaagaaa cgattttgcc cagatacagc gcacggcgaa ctttttcgat gaactcagca
    16741 ttgtcgccag ctggctgcgc ttgcgggcca gagagaactt tagatgcggc aacacgctgc
    16801 tctttcagag aagagatata acgtgcaaac acagactcgg taatcagcga cagcggttcg
    16861 ccgagatcca gcgcgctctg gctggtccat ttgcccgtac ctttgtttgc tgcttcatcc
    16921 agaatcacat caaccaggta gttaccctct tcatcttttt tggtgaagat atctttggtg
    16981 atgtcgatca ggtagctgct cagttcaccg ttattccact cggtaaaggt ctgcgccagt
    17041 tcttcgttgg tgaggttcaa gccaccttta agcagagaat aggcttcagc aatcagctgc
    17101 atatcaccgt attcaatacc gttgtgaacc atcttcacat aatgacctgc accatcggca
    17161 ccaatatagg taacgcacgg ttcgccgtct tcagccacag cggcgatttt ggtcaggatc
    17221 ggcgcaatca gttcataagc ttctttctgc ccaccaggca taatggaagg acctttcagc
    17281 gcaccttctt caccaccgga aacaccggta ccgataaagt taaagccttc tgcagaaagc
    17341 tcacggttac gacgaatggt gtcatggaag aaggtgttac caccatcaat gatgatgtca
    17401 cctttatcga ggtatggctt gagggaatca atagcagcat ccgtgccagc acctgctttc
    17461 accattaaca ggatgcgacg aggcgtttcc agagattcaa caaattcttt caccgtatag
    17521 taaggaacca gtttcttgcc tggattttcg gtaatcactt cttcggtctt ttcacgggaa
    17581 cggttgaaaa tagagacggt ataaccacgg ctttcgatat tgagcgcaag gttgcgcccc
    17641 atcactgcca taccgacgac gccgatctgt tgctttgaca ttgtttactc ctgtcaggat
    17701 accgctgggt ggtatgcggg ttatgcttaa ttatagaata tgcctaataa aaataaatcc
    17761 ataacactta atcagaaaat tattattatc gattcctaac gattgaatac atcagctcct
    17821 ttaatttaga tggcattata cgaaaaaatg ttctcaacat agcattactt attaattcat
    17881 tttttcgaat aaaaccaatt ttatattgat aatacaatac tttatactcg tacaataaat
    17941 atgacaatcc acgtcgagcc ataagattac gaccagttcg catttttaat aaaatatctg
    18001 gaagatttgc aaatcttgca ttatgtacaa ttaataggct ccacaatgca aaatcttgag
    18061 attttctgaa tggaggataa ccaccaacag ctaatactgt attctttcta aaaattacag
    18121 aaggatggct aactgcgctt cgtttcctcg cgaatttaac tatttctcta tgttcgagag
    18181 gcactttgcg tgttgaaata aactcctcag taacagtttc aatttcatca ataaaactgc
    18241 cacatacatc tatttctgaa ttattaatca taaaagaaat ttgtttctca aaccgatgag
    18301 gcaaagaaat atcatcagca tccattcttg ccactaactc attcctacaa gcctttaatc
    18361 cttcatttaa ggcattagcc aatccaacat ttctaggtaa aggtacaaat gttactattt
    18421 tattgccaac atcatcaatg aatgaattta taatatcgat gtgtgtttga tggagttctc
    18481 catctgcaac aattactatt tgatctggct taagtgtttg atcgtgaaaa atagagcgta
    18541 gagccacctc aaaaaattgc ggtagatcat ttttataaat gctaattaaa actgagaatt
    18601 tttctaatct atgattcatt tcattttacc acttcgaccc attaaaccgt cattaatgcc
    18661 ttttaaaaaa aaatataacc ttttattacc atttggaagg aaaataggat ataaaaaaac
    18721 ctttccaatt aatttaacca gactagaaat tttccagtag atgggtacat aatttttatt
    18781 taataaaaga aagatatttc gagtagcata ataatgacga aatgggcttg gcaaaccgac
    18841 agaaagaata tttaagatct taaatcgccc atctccaagt ctatgtgcaa gtaacgcatt
    18901 tttattccta attactttaa acccagcagc tcttaatctc caacaatatt catggtctac
    18961 cgcatcgata aaaagctcat ctttcattcc tccaacaatc aaccaactat tttttggtat
    19021 tagactgcca gaacttaatg tactatctac ctcataataa acttctgtaa gtggtttccc
    19081 tttttttacc cttgctttat ttaattcacc agttacttta tcaaaatctt gtgaaccaac
    19141 taaaccaaca ttgacatttt gtttaagcaa ttttttgtaa caagtaagta actgctctac
    19201 catcttagga tcaggaatac tatcctgatc catttgcaat ataaaatcag cgccattttc
    19261 aaaagcccat ttcattccta tactttgggc ttctgctatg cctaaattat cattgaaatt
    19321 gaatattttt acatcgcctg aagaattttc agcatattta taaccatttg tagagttatt
    19381 gcaaacgaca actttagtaa cttgtctcaa caataattca accgcatttt ttaaatcatt
    19441 atgttctggg ttgtaagcaa ccaaaacggc atatacagtg tccatcttca ccttaaaacc
    19501 ttcatttagc tttcatcttt tttagaacat tacttaatgt cactaataca attattacag
    19561 caacatggtt agagtctaaa atataaggat tagtaattgc ataagaaaca tatagaaaat
    19621 atagcacaca caactcactg tattttatga ttttaatcgt gagaaggaga ttaattaata
    19681 aaaacaaagt aaataaaata acgccaagtt gatttaaaaa ataaactgac tgcaattcat
    19741 aatatatata tgcactataa tcacggatag gagtttgaat tttgatgaca ttacccaaac
    19801 cagaacctat aacaaaattt gatacagact ctgtaagatc attaattaat acagtaaact
    19861 gatcccatct aactcctaaa gaagaatcag ctccatttga tttcatgatt atcaactcaa
    19921 ttgaatatgt aataaaaaaa gggagaatca cagtaagaaa aaccccaaaa ataattttcc
    19981 ttaatttagc gtatcgtgag ttagatttag aacatagtat aatatacata aaaaacaagc
    20041 atatcgaaac aaaatatgca aaattaccag ccactatagt acctatagcc agaataacgg
    20101 ttattgtatt tttgaatcga taatagaaat aatcttttat gactatatgc aacataaagg
    20161 caaatggaat gagagcattt cctttaattt gaactctata gaaaccactt ccatatgtat
    20221 aaacatcacc ataatcattc tccaaaaaat aatgtcttag tgctgaataa tcaccaatac
    20281 catatgtttt tgtcatataa atactaatga tggatataat aaccgcctgt aataccatta
    20341 aatataaaaa tattttaaca atcgagatgg ttccataaga gcagaaataa gcacataata
    20401 taaataatat gataatataa aacctaatta ttatcgctat atcgttaccc ttgatatagg
    20461 aataaataaa atttataaaa agagctaata gaaatattaa aataacagga tagtgatata
    20521 ttccgtttgc aattttcttt gtaaatgaca tgatacaaag acataaaaac ccctccataa
    20581 tccaactata ttgaataaat ggaaagctac gtgtaaggaa aaatataaac ccaaaaaaca
    20641 aaagaacact taaacttttg tcttttgagt tataaaaatc agaagtcatg tttgcactct
    20701 aattagatgg gcttgaggaa gtaatcccta aaatcaattc gctattaata tttcgtatca
    20761 attaataata atatcaaaaa atctaacgat gttcttacag accatgctat tgcggctcca
    20821 acaattcccc aatgataaat aaaaatatat aatatgcata aatatgggat aacttcgagc
    20881 aaatgaataa tagctgtaat ttttgatctt ccactagcct gaactgaaac aaatgggatt
    20941 tgtgcaatgc aattaaaaaa gaaacctatt gcaagaattt ttaatactat acctggcgtc
    21001 ccatgatatg taggtcccat ccaagcggac attataaaat ctgataaaat aattatcaac
    21061 attacaattg gaagtatacc aataaccatt ataaaatatg ataatatttt agtttgcttt
    21121 accgattgca attctgaact taatcttgga aaaatagctc tggacaacgc acttggtaat
    21181 atcgttaagc gttgtatacc ttcagacgga gcagtataaa aagaaacttt atcagccccc
    21241 acaatgtgtg aaagaataaa acgatccata tatgtcataa tagggctaat aatattgcta
    21301 actgttatcc agcttccaaa gccgattaat cttttaactg ttacaatttt tacagacagc
    21361 ccagatgata ttattagttt tcgactaaat ataaaggtca ctataagtga taagactctt
    21421 gccataacta aaccatatat agcacttagt aatcctccat gaaaaaaaca gaaaatcact
    21481 ggtaatccag ccacaaaaga gttgttaatt gattttatta aatttacttt tctgaacttt
    21541 tccatcccct caaaaatccc caaccagact tggtttaaca agtataaggg tatggtagct
    21601 gaaataatat atattgcttt gacagattct acaacatgat tcgcgttaat gtttaataat
    21661 ttaacaatta cattgctact caaaaatagt acactaccgc caatcaagcc caatatagtt
    21721 agaattaccg ttgaagttga aatgatcgct cttaattctt tatgaacatt tttatatatt
    21781 gatacttctc ttataacagc tctggtcaat ccagcatcaa aaatacttgc atatccaact
    21841 aaggcaatag ctaacgtaaa aaggccaaat tgctcggtcc ctagaattct agacagtata
    21901 cctaacgcag gaattgctat taatgatggt ataatatacc cacttatatt ccataaagta
    21961 ttctttacaa tactcacaaa aataattcct tcatgttatg caattcttta gcccttgcat
    22021 ctttaatcga taaaatataa ttattatgtt ctatcgtcgg ccattttatg ctcagaatag
    22081 gatcattcca tacaatccct ctatcactat caggatgata atagttcgtc gttttatata
    22141 aaaattccgc agtctcgctc agcaccaaaa aaccatgtgc aaatccctca gggatccaca
    22201 attgccgctt attctcagca gataaattca ccccaaccca tttaccaaag gtaggcgacg
    22261 atttacgaat atcaacagct acatcaaaaa cctcaccaac aacgcaacgt accagtttcc
    22321 cttgcgcata aggttctaac tgataatgca gcccgcgtaa aacaccttta ctagacttcg
    22381 aatggttatc ctgaacaaat tcaaccttac gtcctacagc ttcttcgaaa actttctgat
    22441 taaagctttc cataaagaaa ccacgctcat caccaaaaac tttcggctcg aaaattaaca
    22501 catcaggaat ttctgtttta attacgttca ttttattaat aacctttaat cattttcagc
    22561 agatactgtc cataagcatt ttttttcagc ggctccgcta atgctttcac ctgttcagca
    22621 tcaataaacc ctttacggta agcaatttct tctgggcagg aaacctttag tccctggcgc
    22681 tcttcaatgg tggcaatgaa gttgcttgct tcaataagac tctgatgtgt ccccgtatcc
    22741 agccatgcat aaccacgccc catcatggca acggataaac gcccctgttc catataaata
    22801 cggttaatat cggtaatttc cagttcacca cgggcagaag gcttaaggtt tttcgccatt
    22861 tcgacaacgt cgttatcata gaaataaagc ccggttaccg cataattact ttttggttgt
    22921 agcggttttt cttccaggct tattgccgta ccgtttttat caaactcaac gacgccgtag
    22981 cgttcaggat cattaacgtg ataggcaaat accgttgcac cactttcttt gttaacagcg
    23041 acatccatta acttcggcag atcatgaccg tagaagatat tatcaccaag aaccaaagca
    23101 caatcatcac caccgataaa ctcttcaccg ataataaacg cctgcgcaag cccatctgga
    23161 gtcggttgca ctttgtactg aagatttagc ccccactggc taccgtcacc tagcagttgt
    23221 tgaaaacgag gagtatcctg tggcgtacta ataatcagaa tatcgcgaat acccgccaac
    23281 atcagtgtag agagcgggta atagatcatc ggcttatcat aaataggtaa tagctgttta
    23341 ctgacagcca tagtcacagg ataaagacgt gtaccagaac cacccgctaa aataatacct
    23401 ttacgcgttt tcatttcatc attcctttta attcatcttg ctccaccatc acgaacaaga
    23461 tgcaaaaact attaaattgc tgtagtcgta attaattcgt tgagcattcg tttcacacca
    23521 acctgccagt caggcaagac aagcgcaaag ttctgctgaa atttttctgt attaaggcga
    23581 gagttatgtg gacgacgagc tggtgtagga taggctgttg ttggtactgc gttgagcttg
    23641 ttgagtgcaa ggggaatacc tgctttgcgc gcctcttcaa aaaccagcgc agcataatcg
    23701 tgccaggttg tggtaccact ggctaccaga tggtacaaac ctgcgacttc cggtttattc
    23761 agtgccacac gaatagcatg tgccgtacaa tcagccagca gctcagcacc tgttggcgca
    23821 ccaaattgat catttatcac agccagttct tcgcgctctt ttgccagacg caacatcgtt
    23881 ttggcgaagt tatttccttt agctgcgtat acccagctgg tacggaaaat aagatgcttc
    23941 gcgcaatgtt cctgtaacgc tttttctccg gctaacttgg tttcaccgta aacatttagc
    24001 ggtgcggttg catccgtctc cagccatggc gtgtcgccat ttccagggaa tacgtagtca
    24061 gttgagtaat gaattaccca agccccaact tcattagcct cttttgcaat tgattcaaca
    24121 ctagtcgcat tgagtaattg tgcaaattcg ggttctgact cagccttatc tactgcggtg
    24181 tgagccgcag cattaacaat aacatcaggt cgaattcttt tgactgtttc agctacacct
    24241 tcaggattac taaaatcacc acaataatca gtggagtgaa catcaagagc aatcaaatta
    24301 cccaaaggtg ccagagcacg ctgtagttcc caacctacct gccctgtttt gccgaaaagg
    24361 aggatattca ttactggcgg ccctcatagt tctgttcaat ccacgattga taagcaccac
    24421 ttttcacatt atcaacccat tttgtattgg acaggtacca ttccaatgtc ttccgaatcc
    24481 cgctctcaaa cgtttcctgc ggtttccagc ccaattcgcg gctaatcttc tctgcatcaa
    24541 tcgcataacg gcgatcgtgt cccgggcgat cggcaacata agtaatttgc tcgcggtaag
    24601 atttctcttt cggtacaatc tcatccagca aatcacaaat agtgagcact acatcgatgt
    24661 ttttcttttc gttgtgtcca ccaatgttat aagtttcacc cgctttacct tcggttacga
    24721 cggtatataa cgcacgcgca tgatcttcaa catacagcca gtcacgaatt tgatcccctt
    24781 tgccataaat aggtaatgcc ttaccttcca gagcattcag aataaccaat ggaatcaatt
    24841 tttccgggaa atgataagga ccataattat tagagcaatt agtcacaatg gttggtaaac
    24901 cataggtacg tttccacgcg cggactaaat gatcgctgga tgcttttgaa gcggaataag
    24961 ggctgcttgg cgcgtaagct gttgtctctg taaataaggg taattcttct gtattattta
    25021 cctcgtcagg atgaggcaaa tcaccataga cttcgtcagt agaaatatga tgaaaacgga
    25081 atctagtttt cttgtcgcta tcaagagcag accaataatt gcgagcggct tccaaaagga
    25141 catatgtacc aacaatattg gtttcaataa atgccgcagg acctgtaatt gaacggtcaa
    25201 catggctttc agcagccagg tgcatcactg catctggctg atgctgagca aaaatccgtg
    25261 ccattgcagc tgcatcgcaa atatccgcat gttcaaaaac atagcgttca gaatcagaaa
    25321 catcagcaag tgattccagg tttccggcgt acgttaattt atcgacatta acaacactat
    25381 cctgcgtatt atttataatg tgacgaacta cagcagaacc aataaatcct gcgccaccag
    25441 taacaagtat tttcacctaa tttattccat attgcttcag agcatgctgt gaaataagcg
    25501 gctctcagtt tgattaatag aagtattaat gcacgctacc gcccctggct ttacagctac
    25561 cagagcactg catgcatgcc tacgatgtga cgagcgttac ccactcgcgc taaacccgaa
    25621 aaattcaaaa gctaattgtc ttaccaatcc gctctggaaa caaggaaaat cctggaaaac
    25681 tttgactaaa atcctattgc taactcgttg ttattctgat tgtttatata aaacaacggc
    25741 aggaatattc gcaacaaatt actttcacca cgaatcttca ctgccgttat aattttctta
    25801 tcaaccgtta catccggtca gattttcatt attcgcttaa cagcttctca atacctttac
    25861 ggaacttcgc cccttctttc aggttgcgca gcccatactt cacaaacgcc tgcatatagc
    25921 ccattttttt accgcagtcg tagctgtcgc cggtcatcag cattgcatca acggactgtt
    25981 ttttcgccag ctcggcaatg gcatcagtca gctgaatacg tccccatgca ccaggctgag
    26041 tacgttcaag ttccggccaa atatcggcag aaagcacata gcgaccaacg gccatgatgt
    26101 ctgagtccag cgtctgcggc tgatccggtt tttcgataaa ttcaacaatg cggctgactt
    26161 taccttcgcg atccagcggt tctttggtct ggatgacgga gtattcagag aggtcacccg
    26221 gcatacgttt tgccagcacc tggctacggc ccgtttcatt gaagcgcgca atcatggcag
    26281 caaggttgta gcgtagcggg tcggcgctgg cgtcgtcgat cacaacgtct ggcagcacca
    26341 cgacaaatgg attgtcacca atggcgggtc gtgcacacaa aatggagtga cctaaaccta
    26401 aaggttcgcc ctgacgcacg ttcataatag tcacgcccgg cgggcagata gattgcactt
    26461 ccgccagtag ttgacgcttc acgcgctgct caaggagaga ttctaattca taagaggtgt
    26521 cgaagtggtt ttcgaccgcg ttcttggacg catgagttac caggaggatt tctttgatcc
    26581 ctgcagccac aatctcgtca acaatgtact gaatcattgg cttgtcgacg atcggtagca
    26641 tctctttggg tatcgcctta gtggcaggca acatatgcat cccaagaccc gctaccggta
    26701 taactgcttt taaattcgtc attattttcc tacctctaag gggctgatag tgcgtaaatt
    26761 attgtcatag gttagccaaa cggtatggct atataccaag cataactttg attaaacctt
    26821 acgataacac tacacaccat cagcatctgg gttactcgga ttactcggaa atccacatac
    26881 tgataattta atcagtacct ctttccgaat aatcgtagtc caacctggtc cttttttctc
    26941 tgactcgtct gcattactca gaaacaaacg ttatgtcgtc ttttttggca tggacgaatt
    27001 catactgcag agttcgatcc agaccttgcg acagcgtata cggtgcaaca aaacctgaag
    27061 aatgcacttt cgttgcgtca aactgtgttg ttgcgcagaa ttttttcacg cgcacagagc
    27121 tgacagcgta ttttttgccc gtaattttgc tcaggatatc aaagcaatat ccacccagca
    27181 ttcctagtgg gtaaggcaag tgcatagaag ggatcttttt gttcaggctt tgttcaactt
    27241 cagcaaccaa ctggttcatg ttcaggtctg gcttatcaac atagttataa acctcataac
    27301 ctgcggcaac attcttcagt ttgtacttga taaactcaac aatgtttcca acataagcca
    27361 tggacttata gttagtccct gcgcccacca tcataaactt gccgccagcg atctgtttca
    27421 gcaagttata gacgttaccg cggttgcgtt caccgaagat aacggtagga cggatgatgg
    27481 ttaatgaacg ttctgttggt gctttgttat accattcacg cagcacttcc tctgcctgcc
    27541 acttactttt gccgtagtgg ttgaaagggt cgtgtggatg gttttcgtca gggttgtgtt
    27601 tgttcaaacc ataaacagca acggaactgg taaagatgat atttttaacg ccattttttt
    27661 ccatggccgc cagcacattg cgggtaccct gaacgttgac atcataatag agagaagtag
    27721 ggctgacgtc atcgcggtgt tccgctgcca gtagtacaac agtgtcaaaa ccggctaacg
    27781 cctggtcgag tgcctgttga tcacgaacat caccaatctg tgtgatttct ggataaaagt
    27841 ggctctgccg tttgtccagg ttcttgatat taaagtcagc aattgccgtt tcaagtagtc
    27901 gggttcctac gaatccggaa gctcctatga gcaaaacgtt attgttcata aatcacttta
    27961 gtctggttgt tacgtaagaa acacaagata aagatgagta ccttccctga gtagtcaatg
    28021 ctgcccagcc ccagctttaa cagttagtgt gaggattata atcttttaga acattatatc
    28081 cagtaagttt atgaatggtc gcaaatctac tctctccgtt ccggcaatct aaagttaatc
    28141 ttttccacat taacaatatg gtgattaatc ctgtcgatgt cgacggagct ttgccctctt
    28201 tcattcatcg catgaacatt tgcaagtgac agcagtgttt cttttttcgc cataaagaca
    28261 ccgcgtacgt ctttgcgcat gtcgaagttc atgctcaatg ctggtccaac tgaggattcc
    28321 tgcatcacct tgatattacg cataaaaaga tgttgcggtt tgttgtgtaa ctccagcgag
    28381 gcacgcttca tctcaatgtt agtcagtgcc acaaaggaga cagcattccc ggcggagatt
    28441 tggatgccgc gcaatttata aacaagatgg gtgttatcca gttgaatatt attcactcgg
    28501 aaattttgcg gtatcgagag atatttgcct ttaattaccc catagccgat taacatcccg
    28561 gcgctattaa tcatttcaat attatcaatc acgaaattgt cacaaccgta aatagcaact
    28621 gtcgcgttat caatgcctgc tttcttactg aaatccggcg tgatattgcg ggctttgata
    28681 ttacggataa caaaatgttt gccattttca acatggatca actgccgaca atccgatccc
    28741 gtgatattcg ccacgacaaa gtttttcact gcctggtctt ccgggtagtt attatcgtag
    28801 gtactccccg caaggcctat gccaattccc cagttgattt tgccattagt acagttgatg
    28861 cgctcgatga catggtcgga gatcaaaata tcactgtcgt taattgccac gttccattca
    28921 atggcgtcgc cctgtaagtc gctgaactta caattggtga tgttggcacc gataatctgg
    28981 ttatgaaatc cctggcgtaa gatggcgtaa ttagcgtggc tgacagtgag gttatcgatg
    29041 gtcaggttgc tcatgacccg tttgtttttg ccgccgatat aaatctgcgt taccgggcca
    29101 aagccgctca tcgccagccc tttgatggtg cagtcagaac cacgcacatc caaggtgatg
    29161 ttatgcatac tgccgccctc ctcccctgtc acctggctgc cgtcctgtaa gacaaatcgc
    29221 cctctgccgt tgccgcgcaa gcttccaaga atgtgtaacg ttttaccggg agggataaag
    29281 atgccggtgt tgatattgtc acaaaccaat ccggcaggca cgacgaccgt atgcccttcg
    29341 ctgaaggctt gtttaaatga ggcgatccag tcgtgtgggt tgtagtcgtt aatgttaacg
    29401 ctttgtcggg cgggaagtgc gcgagcgaaa ggggtatgga ggaaggcaag cgccgagctt
    29461 gccgtcagga aggtgcgtcg ggagagtgtt ttaaatggca tgcgttctcc tctataaagc
    29521 ttgcagcaag ctggcgagtt ctcgattgat cacctgctgg ttaaaatcgt gttcgacttt
    29581 ttcgcgcgca cgtttgacaa ccggagccag ttcgtcggtg cccagttggc taaacgccgc
    29641 cagtcgttgc gccagtgcgc gagcatcgtt ctcaggcacc agccagccgg atttgtcagc
    29701 ctccaccagt tccggtattc cgctatgcag agtagaaacc accggaatac cgaccgccat
    29761 cgcttccatt agcgccaccg gaatgccttc catatcacca tccgcgcccg ttaccgatgg
    29821 caacagaaag acatccgcgt cgtcgagcat cgctttcact tcgtggctcg gtttaaagcc
    29881 cggcatctct accacatctt ccagttgata ttgttcgatg agggtacgca ggcgtcgttc
    29941 ccacgggcca atgccgagga tgcgatagcg aaatgccacg ccctgctctt tcaactgacg
    30001 gcaggcttca atcgccacat gcaggccttt tttctcggtt aagcgtgcga cggaaatgat
    30061 ttccagcggc gttgcgggcg ctttcaccgg acgcgggcta aagcgcgtca tgtccacgcc
    30121 catacgcgat acggcgattt tttccctcgg gcagcccatt ttttgcagcc ttccggccca
    30181 caaatcgctt atcggtaaca tcaggtcgcc acggcgaaac agttgttgat attcgggagt
    30241 gtaatggttg agcacttccc gactggaaat atcaatgccg tggaaaatgg tggcaatttt
    30301 gccgcgaatg acacccagtt cccgtagttt tgctgcggtt acccccgccg ggccaaaatg
    30361 agcgatgaac acatcggcat gaagcggtgt tgcgacctgg ccgcaaatgg cagacaaaat
    30421 caggttccgc gactcggcac catagcgttt gaggttgagc gcctgccagg tatttttacg
    30481 ataaatgccg cgtaacgtct ggctggcgcg gtggcgcagt ttcgctattt tgcccgtagg
    30541 ttcgtcctgt aaccagtggg ttctggcggc aaggttgtat ttcgtccatg ccgcgtgggt
    30601 gttttgtgtg tcgccttttt gcagcgcgag aatctctacc tcaaatccca tatcaataaa
    30661 cgcggtaatt tggttaagga cgaaggtttc tgacgacagc gggaatttca gtaaaaagaa
    30721 gccgaccttc atttcacctc cccgatgcgc tcaagcacag actgcaccat ctgcattcct
    30781 gtctgacgct cacgactgac ggcttcacta agtcgcgcat tcagtgccgg aagctggcct
    30841 aaggtatccg caaccatcgc ttgcaggctg ccgtctaata aatgacggat atcaattgcc
    30901 atctccggta gtcccagctg ctgcataatc ccggcggatt tatgttcgta gttgatggca
    30961 attgccggag tggcaaaatt catcgagata atggcagagt gcaggcgcgt accgacggtg
    31021 agttcacagg caccgagaat tttgcccatt tccagatcgt tgagttcatc catcaccaca
    31081 tggtaacggg caggatcgct gatgtgctgg cgaagattga gcgccatcat gcggtcgtct
    31141 ttgttatagc tgtcgatacc cgtacaggtg gagagtgcaa tgacctgata gccttcgtcg
    31201 agaatgcgat tgaccacccc ggcaaaggct ttttcatacg cttgttgagt ggtgccgagg
    31261 cgtttgtcaa acggtgccag ttcgcgcagg gtaattgcta ccgttttctg ttgtgcggca
    31321 acgtccagcc agtgttgaac agcatagctg gcggtgaagt cttcggtgtg gtgatcgacc
    31381 agccacgcgg tatcgacgcc atgttccact tttgcggtag tgatattgct gcgtttcatc
    31441 agatcaagac tgaccgattc gcgcaggatc agcgcgtcac agtgaccaaa aacgtagttc
    31501 gccagttggt taaattgctc atcctggaag gggccgacgc tgtgaccaat cataaacagc
    31561 ggctttttcg ccataaacgt gcaaagcgca tgttcaaact gcggcacgcc gtagagatcg
    31621 acaaaaaacg atcctccgac ctgaataatg gcgtcgtaac ctgagagcag gcgcacgaag
    31681 tcggtgaatc cctgggcgat ggcgatatta cgcagcttgc cagtgtcggt gacgcgtgag
    31741 agcaatacct ggtgttgata gcggcgacgg aggacttttt taacgcgccc gacaacgccc
    31801 gccgcgctgt tgtgttgttt catttgcagg aacagaggat cgcccattac cgggcggttg
    31861 agtagccagg aagaactgac cggatagcgg ctcatcacgt ccacttcggc ttgtggattg
    31921 aggatgttga tggcatcaag taagccgcgc aagatggcgc tgtcgccacg attgccgcaa
    31981 gtgtggttgc ccagaataag taatttcatt ttttcctcat aaatttgatg ccaagtgagg
    32041 ctgtgtttgt tttgttcgtt gccgcttttt gcctgatgcg acgctgacgc gtcttatcag
    32101 gcctacaaga cccgagcaca gaaccgtagg ccggataagg cgttcacgcc gcatccggca
    32161 ttcagtgcct gatgcgacgc tgacgcgtct tatcaggcct acaggtcccg agcacagaac
    32221 cgtaggatgg ataaggcgtt ttacgccgca tccggcaacc gttgtcggaa ccgaaaacag
    32281 caactcaccc cgcccgtaaa agcattttca ttttttcgct gcgacaaaac tgacgcttca
    32341 cttccaccac cagcggatgg cgcgacagca caatcatcac cacaaacgcc agcacgcccg
    32401 cggctatttg caccgccagc agcatcccca gcgccagttg ccctttcagc acaatgccca
    32461 gcgcataact gaccaccagc gtcggcaacg agagataaaa cggcagccac aaactcagga
    32521 tgtactggcg ataactggaa ccaagaaccg gtttaatcat cacgaagtaa ctcagaatgg
    32581 tgttgataat ctgcaccagc aggaaaccga gcgtgacgcc gattgcgccc gccatctgcc
    32641 caccaataac aatcgccgga ataaacagaa atgttttgaa tacgttgaat ttaaagctga
    32701 tatcgacccg cgctttcgcc atcagcagcg aaccaatcgg gttacccacg gagcgcagta
    32761 gccccaccac acacagaaat tgcagcaccg gaataatgct gttccacttc tcaccaaaga
    32821 ccagtggtac aaagttattc gacaccacca ttagcccgag cagcgccgga aagttgataa
    32881 tccccaccac cgacagcggt ttgtagaagt taatacgcag cttttcggtg tcgtcctgaa
    32941 ttttggcaaa cgccggaaac aacacacggg tgatgattgg gttcagcttc atcggcggca
    33001 caacggccac gttgtaagcc aggttgtagc cccctgccac gcccgcgccg agaatacgcg
    33061 ccagcaccag cgttgaaagg ttggtattga gatagttgat gatgctgtcc gccgtcagcc
    33121 aggcaccaaa gcgtaagttc ggtgccaccg acgccagcga gaaatgcaga ccggggcgat
    33181 aaattttgcg gccaaagtag ccaaacagca gcgttctcac cgcactattg accagataac
    33241 cgaggatcgc ggtcatcgcc agcggccaga aatgggcgct aaccaccgta aaagtgaagc
    33301 ccgccagtac cgcgctggtt tcgatcatgc cgattttgtt gaactccagc tccttttgca
    33361 tcaacgcgcg gaactgctgc ccctggggga tcaccacaaa cgccagcgac aatgttttaa
    33421 tcaacggtgc caggtccggg ttattcagca cgtcgccgat gagatcactc aacaaaaaca
    33481 ccgccagaca caccacaagc cccagcccga cgttcagcca gtacagcgtg gtgagttcaa
    33541 ggtggctgat ttcttttcgc tgaataatcg agttagcgat accgaagtca gaaagcgtat
    33601 ctgccagcgc gatgatcacc agcgacacag taagcaaacc gaactggtgg ttgtcgataa
    33661 tccgcgccag cacggtcatc tgcaccagcc cgaggccgat gatgatcacc gtggcaatcg
    33721 ccgaccactt cgcgccgctg atggtttttt cacgtaagct catatcaata tgccgctttg
    33781 ttaacgaaac ctttgaacac cgtcaggaaa acgattttga tatcgaacca gacgctccat
    33841 tcacggatgt actcaaggtc gaactcgacg cgtttttcca ttttctccag cgtgtcggtt
    33901 tcgccgcgcc agccgttaat ctgcgcccag ccggtaatgc acggtttcac tttatggcgc
    33961 agcatgtagc cttcaatgag ctggcgatac tgttcgttat gcgctactgc gtgcggacgt
    34021 ggaccgacaa tcgacatccc cccggtcagc acattgataa actgcggtaa ttcatccagc
    34081 gaggtgcggc gcagaaagtt ccccacttta gtgacgcgcg gatcgttctg cgtcgcctgg
    34141 gtcaccactt tgtcgttctc catcactttc atggaacgga acttccacac tttgatcggc
    34201 ttgccatcca tgccgtagcg agtctggcgg aaaataaccg gccctggtga actgagtttc
    34261 accgccagcg caatacagca cagcaccggg gagatcagca gcaagatcag cgtcgccagc
    34321 acaatgtctt ccgcacgttt tagcaggcgg ttaaccccgg aaagcggcgt gtcatacagc
    34381 ggcaccaccg gaacgccgtt catctcttcg agacgtgaat ggagaatatt gaaggtaaag
    34441 acatcgggga tcagcagcac cgaacaggtg gtgtccgcca gttgatggac cagttttttc
    34501 actcgcgcgc cgtcgcacat ttgcatcgcg atatagacgt tatgaatctt acccgcttta
    34561 gcatcctcga ccagctgttg cagattgccc gcccagtcgt tagaaacacc gcccggtttc
    34621 gggtcgtggt aaacgcccac cacttcaaac cctaaccacg gctgattacg gaagctctcc
    34681 atcagcattt gtccggcggc taaatccccc gccaccgcga ccatgcgctt gttatagcca
    34741 tgattacgca gccagcccgc cccaatgcga atacatgaac ggcaaaccac cagtccgata
    34801 ctggtcagcc cataccacgc cagccagatt ttcagttgcg tgtcgaaatc attgttgaac
    34861 gccaccagtc cggcgctgaa aatcacgctt aaggtccagt tttgtagcaa cagggcaaat
    34921 tctgtcgctg cccgaacacc gcgccatgag cgataaaaat cggtgatgcc gcccagcatc
    34981 tggaacacca ccagcgtaat caacgccacc aacaggtgca tgtagaggaa tgacagtccg
    35041 ctcacttcgc aaaccagcca tagtccggca aacatgatgg tgatatctga aaagcgttgc
    35101 accatagaga ttaacgatgc attggttttc gctcgcgctt ttttagattt gtcatcgttg
    35161 ttcctgttat tagcccctta cccgcagtgg ggtaagggaa gatccgacat tactcgttca
    35221 gcaacgtcag cagagttcgc gttcgcgctt ccatcagcgg cacatcaccg cgtgattcca
    35281 cattcaaccg caccaccggt tcggtgttgg aggagcgcag attaaagcgc cagtcggcaa
    35341 aggtcatgct gatgccatcg gtgcgatcca ccgccagcgc atcacggcta aaatgctgtt
    35401 ccacgcggtt aatcgcctca acgggttgcg ccagtttgct gttgatctca ccgcttgccg
    35461 gaaacgccgc catccggtcg cgcaccagtt cgcccagcgt ttttcctttc aggcacacca
    35521 gttcggcgac cagcagccac gggatcatgc cgctgtcgca gtaagcgaaa tcacggaagt
    35581 aatggtgggc gctcatttcg ccaccgtaga tggcgtcttc cttgcgcata cgttctttaa
    35641 taaaggcgtg tccggttttc gacatcaccg acgtgccgcc agcggcagtc accacatcaa
    35701 cggtattcca ggagagacgt ggatcgtgga tgatcttcgc gccgggattt ttttcgagga
    35761 acgcttctgc cagcaggccg acaatgtagt agccttcgat aaactgccct ttttcgtcaa
    35821 acaggaagca gcggtcaaaa tcgccatcaa aggcaatgcc catatccgcg ccgtgtttga
    35881 tgaccgcatt gcgggtgtcg tcgcggcatt ccggcagtag cgggttagga ataccgttgg
    35941 ggaaattgcc gtccggcgtg ttgtgcactt tgattaattc cacgggtgcg ccaagggctt
    36001 taaagcgggc ttcaatggcg tccaccaccg gacccgctgc gccattcccg gagttaatca
    36061 ccagcttgag cggcgtgagg tttttgacat tgatataacc gaacaggtga tcaacgtaag
    36121 cgtcacgcag gttgatttgc tgatagcgac cgcgtttggt ttcatcgacg ggaggaaagt
    36181 cgttggcttc agccagacgc tggacgtcgc gcagtccggt atcaccgctg atcgggcgag
    36241 ccccttcgcg caccagcttc atgccgttat aatccatcgg attatggctg gcggtgactt
    36301 caatgccgcc atccacgccg agatggaacg tggcgaaata gatctcttcg gtgccggaca
    36361 taccgatatc cagcacgtca acgcccgcat cctgtaaacc ttttgccagc gccagtttta
    36421 aggtttcgct ggtgagacgg acatcaccgc ctaacacaat ggttttcggt ttgagaaatt
    36481 cgccataggc gcgaccaatg cgccaggcga tatcttcatt cagttcttcg cctaatttcc
    36541 cgcgaatatc ataggcttta aagcaggtta attttttcat gtcgttaccc tttttcaggc
    36601 aatagttggc cctgaccgaa acgaccaatt ttgtttttgt cgttatttac atctcggttt
    36661 cgcattcatt gcctgatgcg acgctgccgc gtcttatcag gactacaaac ccgaaccgta
    36721 ggtcggataa ggcgttcacg ccgcatccga caaacagcgc ctgatgcgac gtttacaccc
    36781 gtccgtagcg atccgcgaag cgcaccacat catcctcttc gagataagag ccggagcgca
    36841 cttcaattaa atcaagcgga attttccccg gattttccag gcagtgcgtc gcccccagcg
    36901 gaatataaat ggactcgttt tcaccaagca gtttgatatc accatcaatg gtgacttttg
    36961 ccgttcccgc gaccactacc cagtgttccg cgcggtgatg gtgcatctgt accgacaaac
    37021 cctcgcccgg tttcacggtg atgcgtttca cctggtagcg gtcgcccgcg tcgatagagt
    37081 catatttgcc ccacggacga tacacttcgc gatgcacccg atgctcatgg cgaccatcgg
    37141 ctttgatctg ctcgaccact tttttcacat cctgtaccgc gttacggtcg gcaatcagca
    37201 ccgcatcttt ggtctgcacc actaccagat ctttcacccc gacggtggtg accaggccag
    37261 attcggcata cacatagctg ttttcagttt tgtgattaat cacatcgccg tggcaaacgt
    37321 tgccctcggc ggtgtgggcg ctgatctccc ataatgaaga ccaggaacca acatcgctcc
    37381 agcccgcatc catcggcacc acaacagcat ctgccgtacg ttccatgacc gcgtaatcca
    37441 ccgactcttc cgggcaggcg agaaacgctt cttcatccac acgaataaaa tcaagatccg
    37501 gatcgacgga gctcatcgct ttttcacagg catcgagaat atccgggcga tattttttca
    37561 gttcttcgag atagcgtccg gcgcggaaca ggaacatgcc gctgttccag taatattcgc
    37621 cgcttgccac ataggcctgg gcggtttcca gattcggttt ttcgacaaac tgcgccactt
    37681 caaaggccac cgtatcctgc tcacccgcag acacttcacc gcgacgaata tagccataac
    37741 cggtttctgg tagatccggc acaatgccaa aggtcaccag cttgcccgct tcggcatatg
    37801 gcatggcatt acgcacggcg gcacggaacg cgtcttcatc ggcaattaca tgatccgccg
    37861 ccagcaccag cattaacggg tcgttctccg ggctatgacg ttttgccgcc agcgccgcca
    37921 gcgcaatggc cggtgcagtg ttacgccctg caggttcgag aataatgttc tcggtgagtt
    37981 tgttcagttg acgcagctgt tccgcgacaa taaagcggtg ctgctcattg caaatcacca
    38041 ccgggctttc gcactccacg ccgttcaggc ggcagatggt ggtttgcagc atggtgagat
    38101 cgcctttcag gcataaaaac tgcttggggt aaagtacgcg ggaaagcggc cataagcggc
    38161 taccggagcc acctgccatc acaactggat agagtttcga ctgcgccata attatccccg
    38221 aatatcattt ataaattgac gtaacacgtt ctctttatcg agcgtgcgtt cggcatattc
    38281 acgtgccacc gtgttgtgtt tgggcagcag gagcgcctga ctaatccccg ccaccagcgc
    38341 ctcgaccgat tccggctcta cgcaaaccgc aatgcccgga aaggtttcgc aaagctgccc
    38401 cagttctgtg tgggcttctg cggtaatcac cgcgttaccg cctaccgcct accgccagaa
    38461 tattggtcag tttcgacggc aatacggcat ctgccgcgcc gcatttttgc accaccagat
    38521 ggcaatcgcc catcttcagc agtgcgggta aagcgtcata cgattgcagc gggaaaaatt
    38581 gcatgttgcg cagtccacgc tgctgcgcca ttttttccag ccgcgctttg ccgccgccct
    38641 gcccgacaat ggcaaaaatc agcggttcat cgcgcaggcg atcggcagct tcaataacgt
    38701 tttccagtcc ctgcttttca ccaatattgc cggagtaaag aatgattttt ttgttatccg
    38761 gtaggccaag ctggttacga agggcatcaa catcggcgtc tgcaacatgc tgaaaacggg
    38821 cgatttccga ccagttgggg aagaaaatga tgttatccgc cgccacgcct ttttcgatgg
    38881 ctttattcat catcgaacgc gaaatcgtgg agacgttatc gacgttatgc agtccgctac
    38941 gttcgaacgc cgttgccagc tgtgccactt tgccgccttt gccttttccg gcaaggccca
    39001 gccccagcat ggcgtccact tcgtaatcct gaatatgcag cacggtacgc gcaccagaga
    39061 gtttcgccag caggcgcatt cccggcgtgc aaaacagcgt tggcaccacg ccgataatgc
    39121 gatccggctt ccagcgacgt tgcgccatca acgggaaaaa actgctgacg gcaaaaccgc
    39181 cgagatgcaa caggcgtttc agggtgctcg gctgttttgg cacgtacagc gggcagcgcc
    39241 acaccgtggc ggccccctct tctcgtttgt agcgctaggc ggaatagttc tcgcccacct
    39301 gccactgcgg gtagtaaggc ggtgcggtaa taacccgcac ctcatgacct tgcgccgcca
    39361 gccattccac catttctccg gtgtatttgc cgatgccggt taactccggc gagtagttaa
    39421 tgccgtagac cagtattttc ataatccggg tactccggca cgcttctcag cgagaaaata
    39481 ggcgcggctg ttagcgtgaa cattgtcact ggcgagcagt gcgtccggcg tcagccagcg
    39541 gtaatcgtca tgctgctcat ccggcagtaa cagttcttct tccgctactc tgaagcgaaa
    39601 accgagcacc acatagtgag tggtgaaatc cgtgccagag aagttatcgt cataaaagtg
    39661 ctgccagaca ccgtaaaact ggcctgctgt tatcggcaga cgcagcccca gttccgccat
    39721 cgtcagccgc tcaaatgcgg cttccagcgt ttcgtctttc tgcacgcgcc ctcccggcac
    39781 aaaccagtaa ccctgcgccg ggcggttggt tcttttgcca ggcagaaact cgccgcgact
    39841 gttctcgaca ataaagtcga gagagacaag cggtgtggag cgcactaccg tggcaaagtc
    39901 ttcctgacgt aaaaacatca ttacccccga aagcggtctt gattctcaag gaaccactgg
    39961 taagtgctgg caagccccgc ttccagtgag atttcgtgat accagccaag ctgatgcagg
    40021 cgcgtcacat caagcagttt gcgcggcgta ccatccggtt tactggcatc aaaaaccacc
    40081 cgacctttgt aacccaccac tttggcgatg gtttgcgcca gctcgcggat agtgcagtca
    40141 acgcccgtgc cgacgttaat gtgcgacagc atcggctggg tgttttccag ccagacttcg
    40201 tgcgccagct ccatcacatg aatgctcgcc gccgccatat catcgacgtg caggaattca
    40261 cgcatcggtg taccgctgcc ccacaccacc acgtccggcg cattctgtgc cgtcgcctcg
    40321 tggaagcgac gcagcaatgc tgggatcaca tgcgaattac tcgggtggaa gttgtcatgc
    40381 gggccataca ggttggtcgg catgaccgag cggtaatctc gtccgtactg gcgattgtaa
    40441 gattcgcaca gtttgatccc ggcgattttg gcaatggcgt aaggctcgtt ggtcggctcc
    40501 agcgtgctct gcaataactc gctttctgcc atcggctgtt ttgccagttt cgggtagatg
    40561 caggacgatc cgagaaacag cagtttgttc acgtcgttct gatgcgcggc gtgaatgatg
    40621 ttgctctcaa tcatcatgtt ctggtagatg aaatccgccg gataggtgtt gttggcaaca
    40681 atgccgccca ctttcgccgc cgccagatag acctggtcaa tgcgttcgct ggcaaagaaa
    40741 tcatgcacgg cgcggctgtc cagcaggttc agctcgtcgc gggtacgtaa taccagctcc
    40801 acatcaccgc gctgttcgag ctgccgcttg atggcagaac cgaccatccc acgatggcca
    40861 gcgataaaaa tgcgttgttt actcatgctt atgactccag cgcgatcgcc acgtcgtagc
    40921 cgtgagattt cagcagagag tgttttttcg ccgcttcgag gtcattagcc accatttcag
    40981 acaccatctc tctgagggtg atttccggtt tccagcccag tttttcgtgc gctttggtcg
    41041 ggtcgtcgag cagcgtttca acttctgccg gacggaagta acgcggatca acggcgataa
    41101 tcacatcacc cggtttaacg cccggcgcgt catgcccggt gacggaaacc acaatgccct
    41161 tctcttcaac acccgtgcct tcaaagcgca gtttgatgcc cagctgtgcc gctgccattt
    41221 ccacgaactg acgtacggag tactgaacgc cggtcacgat aacgaaatct tccggctgtt
    41281 cctgttgcag catcatccac tgcattttta cgtagtcttt ggcgtggccc cagtcacgca
    41341 gggaatccat attgccgagg tacaggcacg actccagccc ctgggcgatg ttggcgattg
    41401 cgcgggtgat tttgcgggta acgaaggttt cgccgcggcg cggggattca tggttgaaga
    41461 gaataccgtt acaagcgtac atgccgtagg attcgcagta gttaacggtg atccagtagg
    41521 cgtacagttt ggcgaccgca tacggagatc gcgggtagaa cggcgtggtt tctttctgcg
    41581 gaatttcctg caccagacca tacagttcag aggtggaagc ctgatagaaa cgagttttct
    41641 tttccagacc gaggaagcgg atcgcctcca gcaggcgcag cgtacccatc gcgtcgacgt
    41701 cagcggtata ttctggtgac tcaaaagaga ccgcaacgtg gctcattgcg cccaggttgt
    41761 acacttcatc cggctgtact tcacgcaaaa tgcgcgtcag gttagaggta tcactcaggt
    41821 cgccataatg cagatggaat ttcgggttgc aggtgtgcgg atcctgataa atgtgatcca
    41881 cgcgctcggt gttgaatgac gatgcgcgac gcttaatacc atgcacctcg taaccttttt
    41941 ccagcagaaa ctctgccagg taagaaccgt cttgtccggt tacaccggtg atgagagcga
    42001 cttttgacat gtcttattcc tctgtatttt tgaatttatt cagtttcaac gcgttcgcgt
    42061 atcaccactg cgggattccc ccggcaaacc acatttaccg gaagcgattt aaaaacactg
    42121 cttcgtgcac ccacgacggt gccgtcgccg attgtgacgc ctggggcaac aaagacatcg
    42181 gttgccagcc agcatttctc gccaatcaca ataggcg
//
LOCUS       DAJTMC010000011        41642 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.011, whole genome shotgun sequence.
ACCESSION   DAJTMC010000011 DAJTMC010000000
VERSION     DAJTMC010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 41642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41642
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.011"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            <1..255
                     /locus_tag="OO130_000552"
     CDS             <1..255
                     /locus_tag="OO130_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HCR5364074.1"
                     /translation="IRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQR
                     YFLALEEGVNLLPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(305..1561)
                     /gene="nupG"
                     /locus_tag="OO130_000553"
     CDS             complement(305..1561)
                     /gene="nupG"
                     /locus_tag="OO130_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HCR5364075.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(1763..2842)
                     /gene="mltC"
                     /locus_tag="OO130_000554"
     CDS             complement(1763..2842)
                     /gene="mltC"
                     /locus_tag="OO130_000554"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HCR5364076.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLQSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(2907..3182)
                     /locus_tag="OO130_000555"
     CDS             complement(2907..3182)
                     /locus_tag="OO130_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HCR5364077.1"
                     /translation="MSRTIFCIFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(3210..4262)
                     /gene="mutY"
                     /locus_tag="OO130_000556"
     CDS             complement(3210..4262)
                     /gene="mutY"
                     /locus_tag="OO130_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HCR5364078.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAAANNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            4423..5142
                     /gene="trmB"
                     /locus_tag="OO130_000557"
     CDS             4423..5142
                     /gene="trmB"
                     /locus_tag="OO130_000557"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HCR5364079.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLNNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            5142..5468
                     /locus_tag="OO130_000558"
     CDS             5142..5468
                     /locus_tag="OO130_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HCR5364080.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLDEVRTSEL
                     FDVWWD"
     gene            5652..6371
                     /locus_tag="OO130_000559"
     CDS             5652..6371
                     /locus_tag="OO130_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HCR5364081.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            6547..7593
                     /gene="ansB"
                     /locus_tag="OO130_000560"
     CDS             6547..7593
                     /gene="ansB"
                     /locus_tag="OO130_000560"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HCR5364082.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            7710..8717
                     /locus_tag="OO130_000561"
     CDS             7710..8717
                     /locus_tag="OO130_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HCR5364083.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQSAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREEAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMKIDDINQANERWKKDTEQLRRNGVI
                     K"
     gene            complement(8872..10008)
                     /gene="hemW"
                     /locus_tag="OO130_000562"
     CDS             complement(8872..10008)
                     /gene="hemW"
                     /locus_tag="OO130_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HCR5364084.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRAEFSAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            complement(10001..10594)
                     /locus_tag="OO130_000563"
     CDS             complement(10001..10594)
                     /locus_tag="OO130_000563"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HCR5364085.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVTQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            complement(10602..10892)
                     /locus_tag="OO130_000564"
     CDS             complement(10602..10892)
                     /locus_tag="OO130_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HCR5364086.1"
                     /translation="MNAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            complement(10889..11455)
                     /gene="yggT"
                     /locus_tag="OO130_000565"
     CDS             complement(10889..11455)
                     /gene="yggT"
                     /locus_tag="OO130_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HCR5364087.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNILLPGMWMAL"
     gene            complement(11473..12177)
                     /gene="yggS"
                     /locus_tag="OO130_000566"
     CDS             complement(11473..12177)
                     /gene="yggS"
                     /locus_tag="OO130_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HCR5364088.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            12195..13175
                     /locus_tag="OO130_000567"
     CDS             12195..13175
                     /locus_tag="OO130_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HCR5364089.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFDALDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRHGISLALRLLPSHCPQ
                     LEQLGAPTVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YIYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQRVGEGRL"
     gene            complement(13351..13767)
                     /gene="ruvX"
                     /locus_tag="OO130_000568"
     CDS             complement(13351..13767)
                     /gene="ruvX"
                     /locus_tag="OO130_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HCR5364090.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(13767..14330)
                     /locus_tag="OO130_000569"
     CDS             complement(13767..14330)
                     /locus_tag="OO130_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HCR5364091.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTASADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            complement(14439..15389)
                     /gene="gshB"
                     /locus_tag="OO130_000570"
     CDS             complement(14439..15389)
                     /gene="gshB"
                     /locus_tag="OO130_000570"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HCR5364092.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVLLMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            complement(15402..16133)
                     /gene="rsmE"
                     /locus_tag="OO130_000571"
     CDS             complement(15402..16133)
                     /gene="rsmE"
                     /locus_tag="OO130_000571"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014226833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HCR5364093.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGKIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            complement(16213..16920)
                     /gene="endA"
                     /locus_tag="OO130_000572"
     CDS             complement(16213..16920)
                     /gene="endA"
                     /locus_tag="OO130_000572"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HCR5364094.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            complement(17015..17512)
                     /locus_tag="OO130_000573"
     CDS             complement(17015..17512)
                     /locus_tag="OO130_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HCR5364095.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MENVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            complement(17589..18983)
                     /gene="galP"
                     /locus_tag="OO130_000574"
     CDS             complement(17589..18983)
                     /gene="galP"
                     /locus_tag="OO130_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HCR5364096.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            complement(19419..20573)
                     /gene="metK"
                     /locus_tag="OO130_000575"
     CDS             complement(19419..20573)
                     /gene="metK"
                     /locus_tag="OO130_000575"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HCR5364097.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATTFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            complement(20877..21092)
                     /locus_tag="OO130_000576"
     CDS             complement(20877..21092)
                     /locus_tag="OO130_000576"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5364098.1"
                     /translation="MGITSAGMQSRDAECGERIFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLISTRLEVATRKKRRVLFI"
     gene            21228..21359
                     /gene="yqgB"
                     /locus_tag="OO130_000577"
     CDS             21228..21359
                     /gene="yqgB"
                     /locus_tag="OO130_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HCR5364099.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            21368..23344
                     /gene="speA"
                     /locus_tag="OO130_000578"
     CDS             21368..23344
                     /gene="speA"
                     /locus_tag="OO130_000578"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HCR5364100.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            23481..24401
                     /gene="speB"
                     /locus_tag="OO130_000579"
     CDS             23481..24401
                     /gene="speB"
                     /locus_tag="OO130_000579"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HCR5364101.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(24607..25365)
                     /gene="loiP"
                     /locus_tag="OO130_000580"
                     /gene_synonym="yggG"
     CDS             complement(24607..25365)
                     /gene="loiP"
                     /locus_tag="OO130_000580"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HCR5364102.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGIK"
     gene            25643..27634
                     /gene="tkt"
                     /locus_tag="OO130_000581"
     CDS             25643..27634
                     /gene="tkt"
                     /locus_tag="OO130_000581"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCR5364103.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIIGMTTFGESAPAELLFEEFGFTVDNVVAKAKELL"
     gene            27948..28391
                     /gene="cmtB"
                     /locus_tag="OO130_000582"
     CDS             27948..28391
                     /gene="cmtB"
                     /locus_tag="OO130_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HCR5364104.1"
                     /translation="MRLSDYFPESSISVIHSAKDWQEAIDFSMVSLLDKNYISENYIQ
                     AIKDSTINNGPYYILAPGVAMPHARPECGALKTGMSLTLLEQGVYFPGNDEPIKLLIG
                     LSAADADSHIGAIQALSELLCEEEILEQLLTASSEKQLADIISHG"
     gene            28419..29807
                     /gene="cmtA"
                     /locus_tag="OO130_000583"
     CDS             28419..29807
                     /gene="cmtA"
                     /locus_tag="OO130_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HCR5364105.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYVDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     FVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWMFNLLDGGLVAGPSLGSIFAYLALTPKGSFLATIAGVTVGTLVSFAITSLI
                     LKMEKTVETESEDEFAQSANAVKAMKQEGAFSLSRVKRIAFVCDTGMGSSAMGATTFR
                     KRLEKAGLAIEVKHYAIENVPADADIVVTHASLEGRVKRVTDKPLILINNYIGDPKLD
                     TLFNQLTAEHKH"
     gene            29822..31099
                     /locus_tag="OO130_000584"
     CDS             29822..31099
                     /locus_tag="OO130_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCR5364106.1"
                     /translation="MKTKVAAIYGKRDVRLREFELPEITDNELLVSVISDSVCLSTWK
                     AALLGSEHKRVPDDLENHPVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSG
                     YSAGYSYEYFGGNATYMIIPEIAINLGCVLPYHGSYFAAASLVEPMCCIIGAYHANYH
                     TTQYVYEHRMGVKPGGNIALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQV
                     QKLLPVDLAASKGIELVYVNTKGMSDPVQTLRALTGDVGFDDIFVYAAVPAVVEMADE
                     LLAEDGCLNFFAGPTDKNFKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQL
                     QPSFMVTHIGGLDAVPDTVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKE
                     LARLVEETHGIWNEQAEKYLLAQFGIDIGEAAQ"
     gene            31096..32061
                     /gene="yggF"
                     /locus_tag="OO130_000585"
     CDS             31096..32061
                     /gene="yggF"
                     /locus_tag="OO130_000585"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HCR5364107.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGVIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQESKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            32083..32592
                     /gene="fumE"
                     /locus_tag="OO130_000586"
     CDS             32083..32592
                     /gene="fumE"
                     /locus_tag="OO130_000586"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HCR5364108.1"
                     /translation="MAKLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQFWHYLNEQD
                     ETPGFADDMTWDFISNVNSITRNAMLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTILKELTP"
     gene            32589..33302
                     /locus_tag="OO130_000587"
     CDS             32589..33302
                     /locus_tag="OO130_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HCR5364109.1"
                     /translation="MKIELTVNGLKIQAQYQNEEIENVHKPLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPATALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(33274..33951)
                     /locus_tag="OO130_000588"
     CDS             complement(33274..33951)
                     /locus_tag="OO130_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HCR5364110.1"
                     /translation="MLTLNQISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PPDDIHP"
     gene            complement(33945..34622)
                     /locus_tag="OO130_000589"
     CDS             complement(33945..34622)
                     /locus_tag="OO130_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HCR5364111.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(34610..35317)
                     /locus_tag="OO130_000590"
     CDS             complement(34610..35317)
                     /locus_tag="OO130_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCR5364112.1"
                     /translation="MHPFTSLILWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLPEWLSGTPRSPERWSHAITLWLRILAIVSTSQL
                     WMQYVPVQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRARGVPLDEG
                     WYQRLRAMPALIIPLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADSTLQRVARY
                     TMILLMLTEFGAWIWLR"
     gene            complement(35318..35896)
                     /locus_tag="OO130_000591"
     CDS             complement(35318..35896)
                     /locus_tag="OO130_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCR5364113.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVAGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(35919..36350)
                     /locus_tag="OO130_000592"
     CDS             complement(35919..36350)
                     /locus_tag="OO130_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR5364114.1"
                     /translation="MMTLPYPYSSSARFYALRLLPGQEVFSQLHAFAQQHQLHAAWIA
                     GCTGSLTDVALRYARQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELAFSRQPCALSGYDELHISPVK"
     gene            36722..37741
                     /gene="epd"
                     /locus_tag="OO130_000593"
     CDS             36722..37741
                     /gene="epd"
                     /locus_tag="OO130_000593"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HCR5364115.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            37791..38954
                     /gene="pgk"
                     /locus_tag="OO130_000594"
     CDS             37791..38954
                     /gene="pgk"
                     /locus_tag="OO130_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HCR5364116.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            39169..40248
                     /gene="fbaA"
                     /locus_tag="OO130_000595"
     CDS             39169..40248
                     /gene="fbaA"
                     /locus_tag="OO130_000595"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HCR5364117.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLSFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            40606..41466
                     /gene="mscS"
                     /locus_tag="OO130_000596"
     CDS             40606..41466
                     /gene="mscS"
                     /locus_tag="OO130_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HCR5364118.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
BASE COUNT        10194 a        10411 c        11089 g         9948 t
ORIGIN      
        1 attcgcggtg aagtggagtt ggtgcggatt cgtgatgccg aagggcgaat tgcggcagaa
       61 ggggcgttgc cttatccacc tggcgtgctt tgcgtggtac ccggggaagt ctggggtggg
      121 gcggttcaac gttatttcct tgcactggaa gaaggggtaa atttgttgcc gggattttcg
      181 ccggagctgc aaggtgttta tagcgaaacc gatgcggatg gcatgaaacg gttgtacggt
      241 tatgtgttga agtaagaata aaaaaaacgg gtcaccttct ggcgacccgt ttttctttgc
      301 gtaattagtg gctaaccgtc tgtgtgcctg tcgggacacg aacgtgttta tatttgaaca
      361 tcgccatgaa cgcgaaggcc agaaccacgg agtaaccagc gaaaatcaac catacggtct
      421 gccagtcggt aatgccgttt tgggtgtaca tctcaacaac tttaccgctc acgatgccgc
      481 cgaggataca gccgaagccg ttagtcatca tcaggaacat cccttgtgca ctggcgcgaa
      541 ttgccgggct aacttctttt tcgacaaaca ccgaaccaga gatgttgaag aagtcgaatg
      601 cgcaaccgta aacgatcatc gacagtacca gcagtacagt accgaacgga gtcgggtcgc
      661 cgtaagcaaa cagcgcaaaa cgcaggatcc acgccacaat actgatcatc attacgttct
      721 taataccgta gcggcttaag aagaacggga tggtcagaat gaacagggtt tcagagatct
      781 gcgaaatcga catgatgatt gacgcatgct gcacaataaa gctgctggca aacatcggat
      841 ctttgtcgaa gctgtgcagg aaggtattac cgaacatgtt ggtaatctgc agttccgcgc
      901 ccagcagcat tgagaagata aagaagattg ccatacgctt gtttttaaac agcgcgaatg
      961 catcgaggcc cagcagggtt gtccagctct gattcgcttg ctgtttagca accggaatat
     1021 gcggcagagt cagggtaaac agaaccagaa tggcggaaag tgctgcgcca atatacagct
     1081 gcatgtggct taattcgaag ccagacaggc tcaccaccca cattgccatg ataaagccga
     1141 tggtgcccca gatacggatt ggcgggaagt cagtaacgat atccatcccg gcattttgca
     1201 ggcgatagta agagatggtg ttgattaacc caagcgttgg catataagca aacgagttaa
     1261 tcaatatcac aaggaacatc gcctccggtg tcgtgacctg tgccgccatg aacagcgtga
     1321 tagcgccaat ggtgtggcaa atggcatata cccatttcgc acttaaccat ttgtcggcca
     1381 caatccccag cagcgcaggc ataaagaccg ctgcgatacc cagtgagcta taaactgcgc
     1441 caatagaagc accgtcaaac ttcagggtaa caaacatata ggagccgagg gtcgtcagcc
     1501 aacttcccca cagacagaac tgcagaaaag agaggatttt cagctgcagc ttaagattca
     1561 tgttaatttc ctcacatcgt gatgcggatg agtgtttgaa aaagcacatt tgaccgtgcg
     1621 agatgatggg attctatcaa cttcgcggac tggttttttg ttacctgtgg caaataattg
     1681 caaacattcc caatttgcaa aacgaaattg tgatgtggat aacatttttg cccctgagca
     1741 tcgtcagggg cggttaatgg aattatcggc ggcggtagga tttttgcgcg gtattcactt
     1801 tataaagata acggcgagat tccgcagagg gatggcgggt cgtcagcgtc tgataaacat
     1861 cgcccggcgt catggtgtta ataatattgg cagcctgaat cttatcattc gaaaagactc
     1921 gcagcacgct gcctgcgccg ccgttatagg cggtgatgac ggcataacgc cgcgatgttg
     1981 ggttatcaat tccgccgaga taaacattgt tcagcatcgc cagatacgcg gtgccggtat
     2041 caatattgct ggcaggatca aacaagaaac tgcggctcgg cgtgccggat ttcccctgcg
     2101 agcggaacac atctttcccg gcagtatgtt gtaccacctg cattaatccc agcgcatcgg
     2161 aacggctgac cgcatacggg ttaaaggaag attcggtctg cataattgcc agaatcagcg
     2221 actcatcaac gccatatttc cgtgacgcct ggcggaccat gccgagatat ttgtgcgcac
     2281 gtttatcaag gtggttcggt accatgttaa tggtgacgct gtagatgata cgcagcccgt
     2341 tactgcggct ttgcagacgg tttttcagca gataatccgc gaagttgctt gcgcgacctt
     2401 cccagcgaat cggctgcccg gtgttgtcca ccacctgacc gtaaaggaaa ggttctttcg
     2461 aaatcgtaat atcatcaacg tcggaataga gatcgaccga actcggatcg tcacccatca
     2521 gcaacgtttt gataattgcc cggcgcaaat gcgcggcagg ttctgtcccg gcgatagttt
     2581 cgatagtaat cgtaccgtca tcgaagttga tgtggctgcg ggtctgatat tgatcggtgt
     2641 atttcacgta gtccttagga ccggcgatca ccacctcttt gaatccccag atgttctcaa
     2701 tattgtgggc aaattgcccc atcagaatat caaaaccgtt ggtatctttg acccaggctt
     2761 cgttataggt atcgcctttt ttggtcgtcg aacaggagat gagcaacggc gcaatcaaag
     2821 ccagcgcgag atattttttc atcattccgg gtgcgtgttg tgtttgtttt tatgcaagtt
     2881 gccggaggcg tgctccggca ctgtttttat tttttatctt ccggcgtata gccctcgata
     2941 tgcacctctt taccctcgaa caggaagttg accatctcct gctcaagcag cttgcggtgc
     3001 tcggcattca tcatgttgag tttcttttca ttaatcagca tggtttgctt gtgctgccac
     3061 tgcgcccagg cttctttgga gatctcgtta tagatgcgtt ttcccagctc gccggggtac
     3121 agctgaaaat cctgaccttc tgcttcacgt tgcaggaaaa tacaaaaaat cgttctgctc
     3181 ataaatcatc ctctttatcg actcacgcgc taaaccggcg cgccagtgcg taactgctgt
     3241 aacaaacgct ccacgggagc cgccaggcca actgacggcg gttgcgctaa gttataccag
     3301 agcgcattgc cttcatccat gcagccggtg aatgacgaca cgggaagcca cataggcaca
     3361 atatctaagt ggaaatggct gaaggtatgc cgaaacgcgg tcagttgcgt caggttatcg
     3421 gcagcaatct gccgttgcgc cagccactgc cgcaaacttt cttcgtcggc aaactgcggg
     3481 aaacagtata aaccgcccca caatccgctc ggcggacgct gcgccagcaa tacttcatct
     3541 tcgtgctgta acagcaaaaa gtagccggtg cgctccggca gcgtctgttt cggttttttg
     3601 cccggataaa gcgaccagct attattggcg gcggcaatac atccgttttg tagcggacag
     3661 agcgaacatt tcggcttcga gcgcgtacaa atcatcgcgc ccaaatccat catcgcctga
     3721 ttaaaccgtt ccacgccgac cgcgggcgtc acctgctcgc tcaaactcca taatttattc
     3781 tcgacctctt ttttcccagg ccagccgctt acagcatagc agcgcgccag cacgcgtttg
     3841 acgttaccgt cgagaatcgg aaagtgctta cccagagaaa gcgagagaat cgcgcctgcg
     3901 gtggaacgcc cgacgcccgg taacgccgcg acttcttcaa aggtttccgg gaatttaccg
     3961 ccgtgtaagg tcgccacttg ttgtgccgct ttatgcagat tgcgcgcgcg ggcgtaatag
     4021 ccaagcccgg tccacaagtg gagaacttcg tcgagcggcg cattggcgag atcggtcacc
     4081 gtcgggaagc gcgccataaa gcgttcaaaa taggggataa cggtcgcaac ctgagtttgt
     4141 tgcaacatca cttctgagag ccatactttg tagggcgtct tgtcaatttg ccagggcagc
     4201 gtttttcgcc cgtatttatc gtaccagtcc agaacctggg ctgaaaattg cgacgcttgc
     4261 atggtcaccg aattcactgt tgttgggggc aagattgcag cacagcgacg gcagggtgta
     4321 aaccggaact ttccgcagcc acggccttat catgcttgca tccggcaact aactttggat
     4381 aatgcccgtt ttcagaacac tttcacaagc gactaaatct ttatgaaaaa cgacgtcatt
     4441 tcaccggaat ttgatgaaaa cggccgccca ctgcgccgta tccgtagttt tgtgcgccgc
     4501 caggggcgac tgaccaaagg ccaggaacat gcgctggaaa actactggcc ggtgatgggc
     4561 gttgagttca gcgaagatat gctggatttc cccgcgcttt ttggccgtga agcgccggtg
     4621 acgcttgaga ttggttttgg catgggggcg tcgctggtgg caatggctaa agatcgccct
     4681 gagcaggact tcctcggcat tgaagtgcat tcaccgggcg ttggtgcgtg cctggcttct
     4741 gcgcatgaag aaggtttaaa caacctgcga gtgatgtgtc acgatgcggt tgaagtgctg
     4801 cataaaatga ttcctgacaa ttcattgcgc atggtgcagc tctttttccc tgacccgtgg
     4861 cacaaagcgc gccataataa acgccgtatc gtacaggtgc cgtttgccga actggtaaaa
     4921 agcaaactgc agctgggggg cgtattccat atggcgaccg actgggaacc ttatgcggaa
     4981 catatgcttg aagtgatgtc ttctattgac ggttataaaa acctgtcaga gagcaatgat
     5041 tacgtaccgc gtccggcatc acgtccggtg acgaaatttg aacaacgtgg tcatcgtctt
     5101 ggtcacggag tatgggactt aatgttcgag agggtgaaat aatggcaaag aaccgtagcc
     5161 gtcgtctgcg taaaaaaatg cacatcgacg aattccagga attaggattt tcggtggcat
     5221 ggcgattccc ggaaggtaca tcggaagaac agattgataa aaccgttgat gattttatta
     5281 acgaggttat cgaaccgaac aaactggcct ttgacggcag cggttatctg gcctgggaag
     5341 gtctgatctg catgcaggaa atcggcaaat gcaccgaaga acatcaggcg attgtgcgta
     5401 agtggctgga agagcgcaaa ctggatgagg tacgcaccag cgaacttttc gacgtttggt
     5461 gggactaaga aagcatacgg gcgatgacaa atgcaaaact gcctgatgcg ctacgcttat
     5521 caggcctgga aagatgcacg atcgagtagg cgggataagg tgtttacgcc gcatccggca
     5581 tggaaaacgc gtactttgtt atcaatctgg ggccagcaaa tgctggcctg attttttctt
     5641 gagggaagac tatgatgcgc aaaatgctgc tggcggcagc actttcagtg acggcaatga
     5701 ccgctcacgc cgactaccag tgcagcgtca cgccgcgtga cgatgtgatt gtcagcccgc
     5761 aaaccgtgca ggtgaagggc gaaaacggca atctggtgat cacgccagac ggcaacgtga
     5821 tgtataacgg taagcaatat tccctgaatg ccgcccagcg cgagcaggcg aaggattatc
     5881 aggctgaact acgcagcacg ctgccgtgga ttgatgaagg cgcgaaaagc cgcgtcgaaa
     5941 aagcccgtat tgcgctggat aaaattatcg ttcaggagat gggcgaaagc agcaaaatgc
     6001 gcagccgtct gaccaaactt gatgcgcaac tgaaagagca gatgaaccgc attatcgaaa
     6061 cgcgcagcga tggcctgacg tttcactata aagccattga tcaggttcgc gccgaaggcc
     6121 agcaattagt gaatcaggca atgggtggaa ttttacagga cagcattaat gaaatgggcg
     6181 cgaaagcggt gctgaaaagc ggcggtaacc cattacagaa tgtgctggga agcctggggg
     6241 ggctgcaatc ctcaatccaa accgagtgga aaaagcagga aaaagatttc cagcagtttg
     6301 gcaaagatgt ttgtagccgc gttgtgactc tggaagatag ccgcaaagcc ctggtcggga
     6361 atttaaaata atcctctatt ttaagacggc ataatacttt tttatgccgt ttaattcttc
     6421 gttttgttac ctgcctctaa ctttgtagag ctccaaaata tattcacgtt gtaaattgtt
     6481 taacgtcaaa tttcccatac agagctaagg gataatgcgt agcgttcacg taactggagg
     6541 aatgaaatgg agtttttcaa aaagacggca cttgccgcac tggttatggg ttttagtggt
     6601 gcagcattgg cattacccaa tatcaccatt ttagcaaccg gcgggaccat tgccggtggt
     6661 ggtgactccg caaccaaatc taactacaca gcgggtaaag ttggcgtaga aaatctggtt
     6721 aatgcggtgc cgcaactaaa agacattgcg aacgttaaag gcgagcaggt agtgaatatc
     6781 ggctcccagg acatgaacga taatgtctgg ctgacactgg cgaaaaaaat taacaccgac
     6841 tgcgataaaa ccgacggctt cgtcattacc cacggtaccg acacgatgga agaaaccgct
     6901 tacttcctcg acctgacggt gaaatgcgac aaaccggtgg tgatggtcgg cgcaatgcgc
     6961 ccgtccacgt ccatgagcgc agacggtcca ttcaacctgt ataacgcggt agtgaccgca
     7021 gctgataaag catccgctaa tcgtggcgtg ctggtggtga tgaacgacac cgtactggac
     7081 ggtcgcgatg taaccaaaac caacaccacc gacgtagcga ccttcaagtc tgttaactac
     7141 ggtcctctgg gatacattca caacggtaag attgactacc aacgtacccc ggcacgtaag
     7201 cacaccagcg atacgccatt cgatgtctct aagctgaatg agctgccgaa agtcggcatc
     7261 gtttataact acgctaacgc atccgatctt ccggctaaag cactggtaga tgcgggctat
     7321 gatggcatcg ttagcgctgg tgtgggtaat ggtaacctgt ataaatccgt gttcgacacc
     7381 ctggcaaccg ccgcgaaaaa cggcactgca gtagtgcgtt cttcccgcgt accgacgggt
     7441 gctaccactc aggatgctga agtggatgat gcgaaatacg gcttcgtcgc ctctggcacg
     7501 ctgaacccgc aaaaagcgcg cgtcctgctg cagctggctc tgacgcaaac caaagatccg
     7561 cagcagatcc agcagatctt caatcagtac taatcacctc gctccggtat cgtgccgggg
     7621 ctttttcact tcagacttac gtccattgcc aattttcatt accctaatga taatcaccgg
     7681 aataaattat ttcgcgtgag gtttttcggg tgaaaaagca atggattgtt ggcacggcac
     7741 tgcttatgtt gatgactggt aatgcctggg cagatggcga accgccaact gaaaatatct
     7801 taaaagatca attcaaaaag cagtatcacg gcattctcaa gcttgatgct atcaccttaa
     7861 aaaatcttga tgctaagggt aatcaggcca cctggtcagc ggaaggcgat gtctcttcca
     7921 gtgacgatct ctatacctgg gtcggtcagt tggcagatta cgaactgctc gaacagacct
     7981 ggacgaaaga taaaccggta aaattctcgg cgatgttaac cagtaaagga acgcccgcgt
     8041 ctggctggtc ggtgaacttt tactcttttc agtcggcagc cagcgatcgt gggcgggtgg
     8101 tcgacgatat caaaacgaat aataaatatc tgatcgtgaa tagcgaagat ttcaattatc
     8161 gctttagtca gcttgaatct gcgttgaata cccagaagaa ctcgattcct gcgctggaaa
     8221 aggaagtgaa agcgcttgat aagcaaatgg tggcggcgca gaaagcagcg gatgcatatt
     8281 ggggaaaaga tgcaaacggt aagcaaatga cccgcgaaga agcctttaaa aaaattcacc
     8341 aacagcgtga tgagtttaat aaacagaacg atagcgaggc gttcgcggtt aaatatgaca
     8401 aagaggttta tcaaccggcg attgcggcat gtcataaaca gagtgaagag tgttatgaag
     8461 tgccgattca gcagaaacga gatttcgata tcaacgaaca acggcgacag acttttctgc
     8521 aatcacaaaa actcagccgt aaattgcagg atgactgggt aacgctcgaa aaagggcaat
     8581 atccgttaac gatgaaagtt tcagaaataa acagtaaaaa agtcgcgatt ttgatgaaaa
     8641 ttgacgatat caaccaagct aatgaacgct ggaaaaaaga taccgaacaa ctgcggcgca
     8701 atggggtgat taagtaagtt tgatatagtg cacgatttgt agcaggatgc ggcgttgacc
     8761 ccgcatccgg caatcaacgt ctgatgcgac gcttgcgcgt cttatcagac ctacaaccgt
     8821 tgccacatgt aggctggata aggcgttcac gccgcatccg gcaatacaag tttactcagc
     8881 cagaaaaagc tccagcagcg aatttaaaaa cagcttccca tgttccgtta tctgccagta
     8941 atccgcacat tctgtgagat aaccctgggc aatcgcctcg tctaactgtg ggcgaatcac
     9001 atcttcgcaa agcccggtat acgcactaaa ctctgcgcgt ggcgcggcct ccagcaagcg
     9061 aaagcggttc ataaagaact caaacggctt atctgcggct tcgacatcac gctggctttc
     9121 cagatacctt ccttgcataa aaccacgagg atgacgcgtt ttagtggtac gcagaatgcg
     9181 cccatccggg aaggtgactt tgccgtgcgc gccgcagcca ataccgatgt agtcaccaaa
     9241 tcgccagtag ttgagattgt gctggcactg ataaccgggt ttggcgtaag cggaagtttc
     9301 atattgctga taacccgctg cggttaataa ctgatgcccc tgttcgaaaa tatcccacag
     9361 cgcgtcatcg tccggcagca ccggtggtcg cgaaccaaac agcgtattgg gttcgatggt
     9421 cagttgatac caggaaagat gcggcggatt cagttcaatg gcctggcgta gatcgccaag
     9481 cgcctcttcc agtgattgat ccggtagtcc atgcatcaaa tcaaggttaa agctacgtaa
     9541 ccctaaaccg ctcgccagct ttgccgctcg tttcgcttct tgcgggccat gaatgcgccc
     9601 aagtcgtttc agcttttctt cgctaaaact ctgcacacca atagagatgc ggttcacacc
     9661 agcacgctga taatcgacaa agcgatcggc ttctaccgta ccagggttcg cttccatagt
     9721 aatttctgca tccgctgcca gcggcaaacg cgcacgcacg ccgtccagca gcgtttgcat
     9781 cgccgggccg gaaagcaggc tcggcgtacc accgccaata aagattgtct ttacttcacg
     9841 gccctgagcg taagccacat cattgtccag atcgcaaagc agatgctgaa cataatcgtc
     9901 gtgcggcact tctcctttca acgcgtgaga gttgaaatcg cagtacgggc atttctgcac
     9961 gcaccacggg atgtgaatgt agagactcag cggcggtaat ttaaccatta cgtaaagcgt
    10021 ccagcagcag tttcaacgcc tgaccacggt gggaaatggc gctcttttct tcgcgggtca
    10081 gttcggcggc ggttttccct tcggaaggta cgaagaagat tggatcataa ccaaagccac
    10141 cagtgcccgc tggttcgcga gtaatcacgc ccggccagct gccgtggcat accagcggag
    10201 tcggatcttc cgcgtgacgc agatatacca gcacgcagtg gaaacgtgcc tgacgttggt
    10261 cgtccggtac gtctttcagt gtttccaaca gtttttgcag attcttttga tcggtcgcgt
    10321 cttcaccgga ataacgcgcg gagtaaatac ccggcgcgcc acccagcgca tctaccgcca
    10381 gaccagagtc gtcggcaatt gccggtaaac cggtcacttt tgccgcatgg cgcgctttca
    10441 gaatcgcgtt ttcgataaag gttaggccgg tttcttcagc ggaatcaacg ccgaggtctg
    10501 tttgtgtcac gatatcaaga ccaaagtcgc taagcagcga cgccagctca cgcactttac
    10561 cggcattgcc ggttgcgagg acaacttttt gcataggata cctaattaat taacgccgcg
    10621 atttctggcg ggatttgttg cggattaatg attttaattt gtttgtggcg gccaagttcg
    10681 cctttttcaa tcaccacctg gcttttggca acccggaatt gcttaccgag aaacttcacc
    10741 agatgactgt tggcctggcc gtcaactggc ggcgcggtaa tggcgacttt aacttcgtcg
    10801 ccatgtaaac cgacaataga atcacggctg gctttcggct gaatatagag ccgtaaaacc
    10861 agaccgtcat cattaactgt tacggcattc ataacgccat ccacatcccc ggcagcagga
    10921 tgtttccggt tgcctgtaat acttctgcga cacccatatt gatgacatac agcagcagaa
    10981 cgaggatcat cggcgagaaa tcaatcccac ccattgccgg tagcaagcgg cgaatcgggc
    11041 gcagcagcgg atcggccagc tgaatcagca cgtattcaat cgggctacga ccctggctta
    11101 cccagctcat aatcgccatc accagcagga cccagaaaat cagcaagccg atggttttca
    11161 gcagaatcag taaaccggca atccagatga ttggcaggaa ggtcaccact ttaaacagca
    11221 caatggcttt gataaaactg agaatatagg caaccagcag cgaggcgctg tcaatgggac
    11281 ccattgccgg aataacgcgg cgcagtggcc cgataattgg ctgcgttacc ttcactacaa
    11341 actgtgagaa ggggttgtaa aaatcacaat gagcccactg catccagatg cgtaataaca
    11401 gcaccatggt atacagctca atgaccgttg aaagcaggaa agtcaacgta ttcatggcgt
    11461 tcctttaatt ccttattttt tagagtaatc acgcgcacca aaaattgcag tgccgatacg
    11521 aaccatcgtg ctacctgccg caatggcggc ttccatatcg tccgacattc ccagagagag
    11581 cgtgtcgata tgcgggtagc gcgttttcag tccggcaaat gctacagcca tttggcgtgc
    11641 aacttcaaac tgccttacat attctgactc aggcgcaggg attgccatca gcccgcgcag
    11701 acgtaaacgc ggtagttcag cgaccgccgc tgccagctcg tccagttctg ccagttgaat
    11761 cccggactta ctgttttcat cactaatatt aatttgaatc agtacgttaa gagggggaag
    11821 ttctgccggg cgctgatcgt tgaggcgggt agcaatgcgc aaacggtcga tggtatgaca
    11881 ccagtcgaaa tgctctgcca ccaggcggct tttattagac tgcaacgggc caataaaatg
    11941 ccattctaat cctgttacgc ccagttcctg aaagtggcga attttatcta ccccttcctg
    12001 aacgtagttt tcaccaaatt gacgctgccc ggcatcaatg gcttctgcga tggcgctcgc
    12061 aggttttgtt ttactgactg caagcagcgt aatttcttct ggagaacggc cgcaacgcgt
    12121 tgcagccgct gagattttgt cccggacctg tgccaggtta tgcgcaatat cgttcatttt
    12181 ccgaggatct tagtatgaat atggaagaaa ttgtggccct tagtgtaaag cataacgtct
    12241 cggatctaca cctgtgcagc gcctggcccg cacgatggcg cattcgcggg ctaatggaag
    12301 ctgcgccgtt tgatgcgctg gacgtcgaag agctactgcg ggagtggctg gatgacgatc
    12361 agcgggcaat attgctggaa aatggccagc tggattttgc cgtgtcgctg gcggaaaacc
    12421 agcgattgcg tggcagcgca ttcgcgcaac ggcatggcat ttcgctggcg ttacggctgt
    12481 taccttcgca ctgcccgcag ctcgaacagc ttggcgcacc aacggtattg ccggaattac
    12541 tcaagagcga gaatggcctg attctggtga cgggggcgac ggggagcggc aaatctacca
    12601 cgctggcggc gatggttggc tatctcaatc aacatgccga tgcgcatatt ctgacgctgg
    12661 aagatcctgt tgaatatatc tatgccagcc agcgatgttt gatccagcag cgggaaattg
    12721 gtttgcactg tatgacgttc gcatcgggat tgcgggccgc attgcgggaa gatcctgatg
    12781 tgattttgct cggagagctg cgtgacagcg agacaatccg tctggcactg acggcggcag
    12841 aaaccgggca tttggtgcta gcaacattac atacgcgtgg tgccgcgcag gcagttgagc
    12901 gactggtgga ttcatttccg gcgcaggaaa aagatcccgt acgtaatcaa ctggcaggta
    12961 gtttacgggc cgtgttgtca caaaagctag aagtggataa acaggaagga cgcgtggcgc
    13021 tgtttgaatt attgattaac actcccgcgg tggggaattt gattcgcgaa gggaaaaccc
    13081 accagttacc gcatgttatt caaaccgggc agcaggtggg gatgataacg tttcagcaga
    13141 gttatcagca gcgggtgggg gaagggcgtt tgtgacgttg ccatgcccga tgcgcaagtt
    13201 tatagggcct ataaaggcaa taaatgtctg atgcgacgct aacgcgtctt atcagaccta
    13261 cagccgctgc cagatgtgtg ctggataagg cgttcacgcc gcatccggca agatggcggt
    13321 aacgactatc cgccaggctt ttagatcgcc ttaatatccc tgctcgaaat agctttcgag
    13381 aataataacc gcagaggcag agtcgacttt gcctttgttg agcgcccgat agccgccctg
    13441 ttcaaacaga ccggaacggg cttccacggt gctaagacgt tcgtcatgga gctttacttc
    13501 aacaccgaaa cgaccatgaa tacggttggc aaatttacgc gctctggcag tcaatggttg
    13561 ctcggtgccg tccatattca gcggcaaacc gacgatgatt tcgtccggct gccactcttt
    13621 cagtaaacgt tcgatgaggt tccagtccgg cgtaccgtcc tgtgctttaa ttgcaggcaa
    13681 agggcgagca gtgccggtaa tgcgttggcc gaccgctacg ccaatgcttt tggtgccgaa
    13741 gtcgaaggcg agtaaggttc cactcatcag gcgtgccctg ccacaccagg catggtgaga
    13801 atatccacac caatcagttt tgccgcctcg cgccagcggt cggcaatcgg cgttttgaac
    13861 agaatattca gatccgccga ggccgttagc cacgcgttat cgagaatttc ttgttccagt
    13921 tgaccttttt cccaggaggc atagcccaga gccaccaata cgtcagacgg ttgtttatcg
    13981 gtgccgagcg tttccagcac atcgcgggaa gtggtcatca ccgtgttgtc tgaaatgcga
    14041 atgctggaag caaaattgga gggcggagta tgcaaaataa acccgcgatc ttcagccagt
    14101 gggccgccga gcataacagg tttatccaga cggattgatt catcacgcgg ctccggcgtg
    14161 atcttcagct tttccagaat cccttcaatt ttgagatttt ccagcggctt gttgacgatg
    14221 atccccattg caccattggt attatgttcg caaatgtaga ccacggaacg acggaaaatc
    14281 ggatcctgga gagcaggcat ggcaataaga aagtgatgct gtaaattcat tgtcagaggt
    14341 tctgtttcct ggttcaaaaa agcaacagcg cccagtatgc gtggaaagaa gggcgctgtc
    14401 actcagagtc tcaacgagat ccttctcgct aaggtgggtt actgctgctg taaacgtgct
    14461 tcgatggcat ccattaacat tccggtgatc gacaccggaa actctgcttc aatctcacga
    14521 atacaggttg ggctggtgac gttaatttca gtcagacggt cgccgatgat atccagacca
    14581 acaaaaatca gccctttttc tttcagcgtc ggcccgatct gacgggcgat tttccagtca
    14641 ctttccgtca gcggccgagg ttcaccgcga ccaccggcag ccagattgcc acgggtttcg
    14701 cccccctgcg gaatacgcgc caggcagtac ggtaccggct cgccatccac caccagcacg
    14761 cgtttgtcgc catctttaat ggctggcagg taattttgcg ccatgcagta gcgagtgcca
    14821 tgctcagtca gggtttcggc aatcacgccg aggtttggat cgccttcttt cacgcggaaa
    14881 atcgacgcgc cgcccatacc gtccagcggc ttaagaatga tgtcgctgtg tttctcccag
    14941 aacgctttta gctgcgcttt attgcgcgta accagcgttt ctggcgttaa gtcagagaac
    15001 caggcggtaa acagtttctc gttacagtcg cgcaggctct gcggcttgtt aacgatcagc
    15061 gtccctttct cttcggcacg ttccagaata taggtcgcgt agataaactc ggtatcaaac
    15121 ggcgggtctt tacgcatcag gagcacatcg agatcggcca gcggcagatc ctgttcaccg
    15181 acgaacgaaa accactcttc gtagttctgc ttcacgttca gcgtgcgggt atgggcgcga
    15241 gcttcgccat tgatcagata cagatcgccc atctccatat agtgaagttc gtaaccacga
    15301 cgctgtgctt ccagcaacat agcgaaactg gaatctttct tgatgttgat gtttgcgatg
    15361 gggtccatca cgatgccgag cttgatcatt attcttctcc gttagcccaa atcgccaaat
    15421 cgtacttgta gcgcggtaat ggcggtgagc gcagttgtct ctgtacgcaa aacgcgaggt
    15481 cccaacagga tatcagtaaa ttgatagcgg gcagtcatgg caatttcatc tgccgataaa
    15541 ccgccttccg ggccaatcag caggcggacg cgttcaaccg gtaacggcaa cgtattgatg
    15601 ctgttactgg cgcgcgggtg aagattcagt ttcagtcctt catcctgctc tgcacaccag
    15661 gcttccagat ccatcgctgg acggatttcc ggcacccggt tacgaccaca ctgctcacag
    15721 gcagcaattg caatcttctg ccactgctgg agcttcttgt tcagacgttc actatccagt
    15781 ttaacgccgc agcgctcaga aaaaagtggc gtaatgaggc ttacaccgag ttcgatcgat
    15841 ttctggatcg taaattccat tttttcacca cgcgacatca cctgaccgag gtgaatatgc
    15901 agcggagatt cgcgatcgtc gatcttgcct tccagcacct tcacttccac gctttttttg
    15961 ctggcgctgg taatttcggc gtcaaagacc tggttgctac cgtcaaacaa ttgcaacgcc
    16021 tgccccggcc ccatgcgcag tacgcgcccg atatggttgg cggcatcttc gcaaagcgcg
    16081 atgtgagaat ggctggtcag tggttcagga tgataaatgc gggggatacg catagttaaa
    16141 aatccgcgtc gtctccccac gcggttgtac gcgtggggta ggggttaaca aaaagagtcc
    16201 cgctagtgta ggttagctct ttcgcgcctg gcaagcgcgt tgcacatacg ggttatgatt
    16261 gccttgcacc ttcgcgatgc gttcatcgcg ctcgcactcc cagtcggtaa ccggatacat
    16321 cttgttccat gcgttgaaca gctgcgtttg ctggcgagag agtgtcaggt tgtattggtc
    16381 gcgcatatag aagtaggtgc gcgcaatggc accgcgtgca cgcgctggtg gttcggcagc
    16441 tttttctttg aaatcgacct tcatggcgca ttgaccgtac tggccttcgc cgccattcca
    16501 ctggctgtac ataaagttgc cgcgatcgcc attcacctca ccgactgacg gctgcaggtt
    16561 atgcatatcg ctttccatct tgcgatagac cggatcttta gcgcagtttt tacgtccacc
    16621 gtcctgccag cactggcgct ggtgaccgaa ctgccaggcg ggaacgacgt gttcccactc
    16681 tacgcggctg gctcggtttt catttttgcg cacctgatag ccgcacgatt gcagatcaac
    16741 aacgcctttt ttgccctgcc agttaatttt acatccgcaa taaaacgtac cgggcgcgtc
    16801 agcgtggact tttaccgccg cggctttcgc ctgagaaaaa ctattgatac cttcggccaa
    16861 cgccgggccg gaaaatgctg cgctcagtac caccgcagca atagacaaat aacggtacat
    16921 cacttactcc gtgttaaaac gagccagcaa cgtagcgaaa gttgttttgg tatgcaatca
    16981 gccagatcag gaaagttcct gatagttcag atggttattt tgcgaccagt tgttcgccgc
    17041 agtgaacaca gcgatagacg gcttcgccac gaactacgcg attatggcgg cgtacggtaa
    17101 gctgatgctc ctggcacttg cagcggtagg ggaaggtgtt gcgacgcacg gattgcagtt
    17161 cgaactgatg cgtacgacgg gcgggaacac ccagcacgtt ttccatcatc cacttccact
    17221 ctttgccatg tggtgctacg cgcccgaaat gtttccatac cagcaaatgt gccagttcgt
    17281 gcggcaccac ttcttcaata aaggcttcac tgttttccaa cagcaaaacg ggattgaggc
    17341 ggatttcata gctttccagc caggctgttc cggcggaggt gccgcgctgg gtataagaga
    17401 gttttggctc cgggtagtta cgccccaatt tcaggttagc ctgggcgagt ttttcccgca
    17461 ggcgacgcat aacggcctgt tggatggcga tagggagacg ggatgttttc atagcggcag
    17521 aggatagagc gaagaaaaga tgactgcaag aggtggcttc ctccgcgatg ggaggaagct
    17581 tggggagatt aatcgtgagc gcctatttcg cgcagtttac gacctttcat cagattacgt
    17641 tcaatatgtt ccagcgaaac gtgtttggtt tccggtacca gccacaatgt cagcaggata
    17701 aacagtacgt tcagagccgc atacacccag aaggtgttgg cgttacccag tgtgttgagc
    17761 atggtcagga acgttgcgcc aacgatcatg ttggcaatcc agttggtggc ggtagagcag
    17821 gtgatgccaa aatcgcggcc tttcagcggc tgaatttcgg agcacagtac ccaaatcagc
    17881 ggaccggcac tcatggcaaa accgacaata aacatcagca gcatggcgat ggcgaaatac
    17941 tgcgctgacg gagagtgaat accgatatgc atcattgtac cgagtacgcc catgccagca
    18001 gccatcacca ggaagcccag cgttagcgtt ggtttacgtc cccagcggtc aacaaggccg
    18061 attgcgataa aggtggcaag tacgttggtc aggccgacaa tcactgtccc ccacatttgc
    18121 tcggtagtgt tggtataacc cgccagttcg aagattttcg gcgcgtaata catgatgacg
    18181 ttcatcccgg tgaattgctg cattacctgt aacagtacgc caaggaacac cgcgcggcgg
    18241 aagttgctat tctctttaaa cagcgcccag ccactctgtt taacctgcaa actttcacgg
    18301 atttcatcca gttcgcgttt cgcttccgcg ctggtgtcac gcaggcgtag cagcacgcgt
    18361 tcggcatcaa caaaacggcg tttggcggca aaccaacgtg ggctgtctgg caggaagaag
    18421 acaccaatca gcagcaaaat tgccgggatg ataatcacac ccagcatcca gcgccatgca
    18481 ccggtgtagc tgaaggcggt atcagaaaga taagcaccga ggatcccgat agtgatcatc
    18541 aactgataca tcgagatcat actgccacga attttttccg gcgcaatttc agagaggtac
    18601 agcggtgcgg tataagaggc cacacccacc gccagcccca gtagaacgcg ggaaagaatc
    18661 agtacttcaa cgtttggcgc agccgcagag aacagcgaac cggcaacaaa caaaattgcg
    18721 ccgatcatca ggctcttttt gcgcccgagt ttaaaggaga gccagccgct gcccaccgca
    18781 ccgactgccg caccgaacat catggagctt acgacccatt cttgcgtgtg cgaagtaatc
    18841 tggaattcat ctgcaataaa cggcagtgcg ccagcaatta caccgatatc caggccaaag
    18901 agtaatcccg ccagagcggc aaggaagcag acgaaaaacg tcattgcctt gtttgaccgc
    18961 ccctgttttt tagcgtcagg catgatgccc tccaatatgg ttatttttta ttgtgaatta
    19021 agataggtga gtacgacgta aaaagatgtg aagcaaatca catcagtgta atcgcttaca
    19081 ctaacattta tcttagttat ggtttaagtg catgaaaata attgagttat atcatcatag
    19141 attaaacgct gttatctgca attaagactt tactgaaaag aaatgtaaca actgtgaaaa
    19201 ctgaataata atatgaaaat attacaaata tttggctggt gttattggtg taatcggttt
    19261 cagtagtgat gataatttgc gataaaacgt gttgaggaag aatgatgtta ttgtgcgggc
    19321 agctttttgc cggatgcggc gtgaatgcct atccaccgcg gtgaggtaaa agaaaaaggc
    19381 cagcgcgagg ctggcctttg aaagcaggtg aagaaagatt acttcagacc ggcagcatcg
    19441 cgcagcagct gcgctttgtc ggttttttcc cacgggaaat gttcacgacc aaagtgaccg
    19501 tatgctgcgg tttctttgta gatcgggtgc agcagatcca gcatctgaat cagaccgtat
    19561 gggcgcaggt cgaagaactc acgtaccagc agagtcagtt gttcagaagg cactttctca
    19621 gtaccgaaag tttctaccat gatggaggtc ggttcagcca cgccgattgc gtaggaaacc
    19681 tgaatttcac aacgatcggc caggccagca gcaacgatgt ttttcgcgac ataacgtgct
    19741 gcgtaggctg cggaacggtc cacttttgat ggatctttac cagagaatgc tccgccaccg
    19801 tgacgtgcca tgccgccgta ggtatcaacg ataattttac gaccagtcag accgcagtca
    19861 cccatcgggc caccgataac aaaacgaccg gtcgggttga tgaagaatgt ggtggcagaa
    19921 gtcagccatt cagcgggcag aattggcttg atgatctctt ccattaccgc ttcttgcagc
    19981 gatttctggt cgatctcttc agagtgctga gtggaaagca cgacagcatc aataccaacg
    20041 attttgccgt cgtcatactg gaaagtcacc tggcttttcg cgtccgggcg cagccacggc
    20101 agagtgccgt ttttacgcac ttcagcctga cgctgtacca gacggtgtgc ataggtgata
    20161 ggtgctggca tcagcacgtc ggtttcatta gttgcgtagc caaacatcag accctggtca
    20221 cccgcgccct gttccagcgg atcggcacgg tcaacgccct ggttgatgtc aggagactgt
    20281 ttgccgatag cgctcagaac cgcacaggag ttagcgtcaa agcccatgtc ggaatgcaca
    20341 tagccaattt cgcgaacggt gttacgggtg atctcttcga tgtctaccca ggcgctggtg
    20401 gtgatttcgc cgccaactaa aaccatgccg gtttttacgt aggtttcgca agcaacgcgt
    20461 gctttcggat cctgttcgag gatcgcgtct aaaacggcat cagagatttg gtcagcaatt
    20521 ttgtcaggat gcccttcaga gacggactcg gacgtaaaaa ggtgttttgc catatttaat
    20581 atcacctaaa gagaatttgg ttagctcaaa ctgttgtgtg gattttctgt ggtagcggat
    20641 cctaccacga ctctgcaggt taaaaacact ggcagtctga gtgttaatcg gtatggatgg
    20701 attaacatct ggatggctat tttaggtcaa ttcttcaccc gatttccact tttttttgaa
    20761 tcgtgtctca ttctgttaaa aacgtggctg gaaatttttt ctgacaatgc cggcattctg
    20821 cgtatttata ttttgcaatt ttctgccatt gtggggtata aaacgcggcg cgcggcttaa
    20881 ataaaaagca cacgacgttt ctttcgtgtt gccacttcca gccgggttga aatcagagtt
    20941 ttggcttgtg ggttcgtctt aacaggcggc cgtggaggtg atacgaaata atgaaccgta
    21001 gtctgctgct taacctgtct caccgttctg gtgaagattc gttccccgca ctctgcatct
    21061 ctgctttgca tacctgccga tgttataccc atctcggcgc ttctcaggat tcaagagctg
    21121 gttacagtta ctgaggactg aacaagggcg ctcttgtaaa aacaagagtt ttctcgtggt
    21181 ttcgccgaac tgtcacactt acgttcggtt atgtgcttaa taatgttatg aaaaagaaac
    21241 cggttgcgca gttggagcgt cagcattcac tgctggaaaa tccatgtgct tatgggttgt
    21301 tatcgcagtt ccaggctgcg atagtcgtta actgttttac acttaataaa ataatttgag
    21361 gttcgctatg tctgacgaca tgtctatggg tttgccttcg tcagcgggcg aacacggtgt
    21421 actacgctcc atgcaggagg ttgcaatgag ctcccaggaa gccagcaaga tgctgcgtac
    21481 ttacaatatt gcctggtggg gcaataacta ctatgacgtt aacgagctgg gccacatcag
    21541 cgtgtgcccg gacccggacg tcccggaagc tcgcgtcgat ctcgcgcagt tagtgaaaac
    21601 tcgtgaagca cagggtcagc gtctgcctgc actgttctgt ttcccacaga tcctgcagca
    21661 ccgtttgcgt tccattaacg ccgcgttcaa acgtgcgagg gaatcctacg gctataacgg
    21721 cgattacttc cttgtttatc cgatcaaagt taaccagcac cgccgcgtga ttgagtccct
    21781 gattcattcg ggcgaaccgc tgggtctgga agccggttcc aaagccgagt tgatggcagt
    21841 gctggcacat gctggcatga cccgtagcgt catcgtctgc aacggttata aagaccgcga
    21901 atatatccgc ctggcattaa ttggcgagaa gatggggcac aaggtctatc tggtcattga
    21961 gaagatgtca gaaatcgcca ttgtgctgga tgaagcagaa cgtctgaatg tcgttcctcg
    22021 tctgggcgtg cgtgcacgtc tggcttcgca gggctcgggt aaatggcagt cttccggcgg
    22081 ggaaaaatcg aagttcggcc tggctgcgac tcaggtactg caactggttg aaacgctgcg
    22141 tgaagctggg cgtctcgaca gcctgcaact actgcacttc cacctcggtt cgcagatggc
    22201 gaatattcgc gatatcgcca cgggcgttcg tgaatccgcg cgtttctatg tggaactgca
    22261 caagctgggc gtcaatattc agtgcttcga cgtcggcggc ggtctgggcg tggactatga
    22321 aggcacccgt tcgcagtctg actgctccgt aaactacggc ctcaatgaat atgccaacaa
    22381 catcatctgg gcgattggtg atgcgtgtga agaaaacggt ctgccgcatc cgacggtaat
    22441 caccgaatcg ggtcgtgcgg tgactgcgca tcacaccgtg ctggtgtcta atatcatcgg
    22501 cgtggaacgt aacgaataca cggtgccgac cgcgcctgca gaagatgcgc cgcgcgcgct
    22561 gcaaagcatg tgggaaacct ggcaggagat gcacgaaccg ggaactcgcc gttctctgcg
    22621 tgaatggtta cacgacagtc agatggatct gcacgacatt catatcggct actcttccgg
    22681 cacctttagc ctgcaagaac gtgcatgggc agaacaactc tatctgagca tgtgccatga
    22741 agtgcagaag caactggatc cgcaaaaccg tgcgcatcgt ccgataatcg acgaactgca
    22801 ggaacgtatg gcggacaaaa tgtacgtcaa tttctcgctg ttccagtcaa tgccggacgc
    22861 atgggggatc gaccagttgt tcccggttct gccgctggaa gggctggatc aagtgccgga
    22921 acgtcgcgct gtgctgctgg atattacctg tgactctgac ggtgctatcg accactatat
    22981 tgatggtgat ggtattgcca cgacaatgcc aatgccggag tacgatccag agaatccgcc
    23041 gatgctcggt ttctttatgg tcggcgcata tcaggagatc ctcggcaaca tgcacaacct
    23101 gttcggtgat accgaagcgg ttgacgtgtt cgtcttccct gacggtagtg tagaagtgga
    23161 actgtctgac gaaggcgata ccgttgcgga tatgctgcaa tatgtgcagc tcgatccgaa
    23221 aacgctgtta actcagttcc gcgatcaagt gaagaaaacc gatcttgatg ctgaactgca
    23281 acaacagttc cttgaagagt tcgaggcagg tttgtacggt tatacttatc tggaagatga
    23341 gtaagtccgt attacttgaa tccgcttaat ttagcggtga taatccgcca caatttattg
    23401 tgacaaatcc aacccttcct cgtcgggcct aacgacgcgg aagggttttt ttatatcgac
    23461 tttgtaatag gagtccatcc atgagcacct taggtcatca atacgataac tcactggttt
    23521 ccaacgcctt tggtttttta cgcctgccga tgaacttcca gccgtatgac agcgatgctg
    23581 actgggtgat tactggtgtg ccgttcgata tggccacttc tggtcgtgcg gggggacgcc
    23641 acggtccggc agcgatccgt caggtttcga cgaatcttgc ctgggaacac aatcgcttcc
    23701 cgtggaattt cgacatgcgt gagcgcctga acgtcgtgga ctgcggcgat ctggtatatg
    23761 cctttggcga tgcccgtgag atgagcgaaa aactgcaggc acacgccgag aagctgctgg
    23821 ctgccggtaa gcgtatgctc tctttcggtg gtgaccactt tgttacgctg ccgctgctgc
    23881 gtgctcatgc gaagcatttc ggcaaaatgg cgctggtaca ctttgacgcc cacaccgata
    23941 cctacgcgaa cggttgtgaa tttgaccacg gcaccatgtt ctataccgcg ccgaaagaag
    24001 gtctgatcga cccgaatcat tccgtgcaga ttggtattcg taccgagttt gataaagaca
    24061 acggttttac cgtgctggac gcctgccagg tgaacgatcg cagcgtggat gacgttatcg
    24121 cccaagtgaa acagattgtg ggtgatatgc cggtttacct gacctttgat atcgactgcc
    24181 tagatcctgc tttcgcgccg ggaaccggta cgccagtgat tggcggcctg acctccgatc
    24241 gcgccattaa actggtacgc ggcctgaaag atctcaacat tgttgggatg gacgtagtgg
    24301 aagtggctcc ggcatacgat cagtcggaaa tcaccgctct ggctgcggcg acgctggcgc
    24361 tggagatgct gtatattcag gcggcgaaaa agggcgagta agcaccagat gcgatgcgca
    24421 cgggtaaaac gtgccattaa tgtcggatgc ggcgtgaacg ccttatccga cctacgttcg
    24481 gcacccgtag gccggataag atgcgccagc atcgcatccg gcaatgcgca caaggtaaca
    24541 aatgtgccat taatgtcaga tgcggcgtga acgccttatc tgacctacgt tcgacaccac
    24601 caggctttac ttaatcccat ccgcgctcat gcgatcgcga atatgctggg cgcgttcggc
    24661 ggatgccgga tggtcgtcaa acattgagct ttggcggcct tcttccagtt tcgccagttt
    24721 ttcaaagctg gtggcaagac ctgccgggct gatgccgcgt tggcgcagaa gatcgtaaga
    24781 ataatcatcg gcttctgctt cctggcgctg ggagaattgc gaattgacta atttctcgcc
    24841 cagatcacca agttgtgatt gagataaact gccgacaatc ccgcccgcag aggccgcagc
    24901 tactcgcacg gcatttgtac caagcgccac ctgcatccct ttcttcacat ggcctaacgc
    24961 cacgtgcccc atctcgtgac cgatcaccgc ttcgacttcg ttatccgtca tcatatccat
    25021 cagcccgcta tagacgcgga tacagccgtt agccattgca aaggcgttca catccttcgc
    25081 catatacact ttgtaattca ccggctgacc gttgatattg ttgcctagcg cattggcaat
    25141 agttgtcaga cgtttagcgt attcgctatt ggctggcgca atcgtcgcct tgctgtccat
    25201 ctcctgacat gcctgatcgc tcagggtttt cacctgcgca tcactcaaac tgtaagcctg
    25261 aaaagcttcc gctcctgatg agagcagtcc gttggagtcc atattctggc aaccagtcag
    25321 taccgttgcc acgctcattg ctaccaataa ggcgcgaatt ttcatgtttt ctcttccgca
    25381 caaggtcagg attattctaa aaatgcgccg tttgcaggtg aatcgacgct cagtctcagt
    25441 ataaggaaaa gcgcagcgga cgggcgagta gattgcgcaa catgcgagca tgatccagag
    25501 atttctgaag cggcaaaagg atgttccatg tacatgacgc gcggcttgcg gtaaattgtt
    25561 ggcaaatttt ccggcgtagc ccaaaacgcg ctgtcgtcaa gtcgttaagg gcgtgccctt
    25621 catcatccga tctggagtca aaatgtcctc acgtaaagag cttgccaatg ctattcgtgc
    25681 gctgagcatg gacgcagtac agaaagccaa atccggtcac ccgggtgccc ctatgggtat
    25741 ggctgacatt gccgaagtcc tgtggcgtga tttcctgaaa cacaacccgc agaatccgtc
    25801 ctgggctgac cgtgaccgct tcgtgctgtc caacggccac ggctccatgc tgatctacag
    25861 cctgctgcac ctcaccggtt acgatctgcc gatggaagaa ctgaaaaact tccgtcagct
    25921 gcactctaaa actccgggcc acccggaagt gggttacacc gctggtgtgg aaaccaccac
    25981 cggtccgctg ggtcagggta ttgccaacgc agtcggtatg gcgattgcag aaaaaacgct
    26041 ggcggcgcag tttaaccgtc cgggccacga cattgtcgac cactacacct acgccttcat
    26101 gggcgacggc tgcatgatgg aaggcatctc ccacgaagtt tgctctctgg cgggtacgct
    26161 gaagctgggt aaactgattg cgttctacga tgacaacggt atctctattg atggtcacgt
    26221 tgaaggctgg ttcaccgacg acaccgcaat gcgtttcgaa gcttacggct ggcacgttat
    26281 tcgcgacatc gacggtcatg acgcggcatc catcaaacgc gcagtagaag aagcgcgcgc
    26341 agtgactgac aaaccgtccc tgctgatgtg caaaaccatc atcggtttcg gttccccgaa
    26401 caaagccggt actcacgact cccacggtgc gccgctgggc gacgctgaaa ttgccctgac
    26461 ccgcgaacag ctgggctgga aatatgcacc gttcgaaatc ccgtctgaaa tctatgctca
    26521 gtgggatgcg aaagaagcag gccaggcgaa agaatccgca tggaacgaga aattcgctgc
    26581 ttacgcgaaa gcttatccgc aggaagccgc tgaatttacc cgccgtatga aaggcgaaat
    26641 gccgtctgac ttcgacgcga aagcaaaaga gttcattgct aaactgcagg ctaacccggc
    26701 gaaaatcgcc agccgtaaag cgtctcagaa tgctatcgaa gctttcggtc cgctgttgcc
    26761 ggaattcctc ggcggttctg ctgacctggc accgtctaac ctgaccctgt ggtctggttc
    26821 taaagcaatc aacgaagatg ctgcgggtaa ctacatccac tacggtgttc gcgagttcgg
    26881 tatgaccgcg attgctaacg gtatctccct gcacggtggt ttcctgccgt acacctccac
    26941 cttcctgatg ttcgtggaat acgcacgtaa cgccgtacgt atggctgcgc tgatgaaaca
    27001 gcgtcaggtg atggtttaca cccacgactc catcggtctg ggcgaagatg gcccgactca
    27061 ccagccggta gagcaggtcg cttctctgcg cgtaaccccg aacatgtcta catggcgtcc
    27121 gtgtgaccag gttgaatccg cggtcgcgtg gaaatacggt gttgagcgtc aggacggccc
    27181 gaccgcactg atcctctccc gtcagaacct ggcgcagcag gaacgaactg aagagcaact
    27241 ggcaaacatc gcgcgcggtg gttatgtgct gaaagactgc gccggtcagc cggaactgat
    27301 tttcatcgct accggttcag aagttgaact ggctgttgct gcctacgaaa aactgactgc
    27361 cgaaggcgtg aaagcgcgcg tggtgtccat gccgtctacc gacgcatttg acaagcagga
    27421 tgctgcttac cgtgaatccg tactgccgaa agcggttact gcacgcgttg cggtagaagc
    27481 gggtattgct gactactggt acaagtatgt tggcctgaat ggcgctatca tcggtatgac
    27541 caccttcggt gagtcagctc cggcagagtt gctattcgaa gagttcggct tcaccgtcga
    27601 caacgtcgtt gcgaaagcaa aagaactgct gtaattagca tttcgggtaa aaaggtcgct
    27661 tcggcgacct tttttattac cttgatatgt ccgtttgcgg acaagcaata gataaagcgt
    27721 gttgcagatc acaaatattt atatgcaata aatatcaatt atgtaatatg catcacgata
    27781 tgcgtattga catttgttgt tatatctata actcaatgtt atataagaaa ttaataattc
    27841 actgttttca aaacaccggt ttccctgctc aattgctttc attaaaccgc acgagtattt
    27901 tatttttacc gccggacggt accctaaaaa taaaataagg ataatttatg cggcttagtg
    27961 actattttcc agaatcatca atctcagtta tacattcagc aaaagattgg caggaagcta
    28021 tcgatttctc gatggtatca ttgctggata aaaactatat cagcgagaat tacattcagg
    28081 caattaaaga ttccaccatt aacaatggcc cttattatat tctcgcacca ggcgtggcaa
    28141 tgcctcatgc gcgaccggaa tgtggggcgc ttaaaaccgg gatgtctttg acattacttg
    28201 aacaaggtgt ttattttccg gggaatgacg aaccaattaa attactcatc ggactctctg
    28261 ctgccgatgc cgattcgcac attggcgcta ttcaggcgtt aagtgaatta ctgtgcgaag
    28321 aagaaatact cgaacaactc ttaacagcat catcagaaaa acaattagcg gacattatca
    28381 gccacggata attactctct cctctacagg ataatattat ggaaaacaag tctgctcgtg
    28441 caaaggtcca ggcttttggg ggctttttga ctgcaatggt catccccaat attggtgctt
    28501 ttattgcctg gggttttatt actgcgttat ttattcccac cggttggctg cctaacgaac
    28561 atttcgccaa aattgtcggc ccgatgatta cctatttatt gcccgtgatg attggttcta
    28621 caggtggtca tctggtcggc ggtaaacgcg gggcggtcat gggcggaata ggtactattg
    28681 gtgtgatcgt tggcgcagag atcccgatgt tccttggctc aatgatcatg gggccgctcg
    28741 gtgggttggt cataaaatat gtcgataagg cactggaaaa acgcatacct gccggttttg
    28801 agatggttat caataacttc tcattaggta tcgcggggat gctcctttgt ctgctgggtt
    28861 ttgaagtgat cggcccggcg gtgttaattg ccaatacttt cgtcaaagag tgtatcgagg
    28921 cgctggtaca tgcgggttat ctgcctctgc tgtcagtcat caatgaaccg gcgaaagtgc
    28981 ttttccttaa taatgcgatc gatcagggcg tctattatcc gctgggaatg caacaggctt
    29041 cggttaacgg taaatccatc ttctttatgg tggcctctaa cccaggtccg ggcctggggc
    29101 tgctgctggc gtttaccttg tttggtaaag ggatgagtaa acgttctgcg cccggggcga
    29161 tgattattca cttcctcggt gggatccacg aactgtattt cccatatgtg ctgatgaagc
    29221 cgctgactat tattgccatg attgcgggcg gtatgtctgg tacctggatg tttaacttac
    29281 tggacggtgg tctggtggct ggcccaagtc tggggtctat ctttgcttac ctggcactga
    29341 cgccgaaagg ctcgttcctg gcgacaattg ccggtgttac ggtaggtacc ctggtgtcct
    29401 ttgctattac ttcgctgata ctgaagatgg aaaaaacggt ggaaacggag agcgaagatg
    29461 agtttgctca gtcagccaat gcggttaagg cgatgaaaca agagggtgca ttctcgttaa
    29521 gcagggttaa gcgtatcgcc tttgtttgcg atacggggat gggctccagt gcgatgggcg
    29581 cgaccacctt ccgtaaacgc ctggaaaaag cggggctggc aatagaagta aaacattacg
    29641 cgatagaaaa cgtgcctgcg gatgccgata tcgtcgtcac tcacgccagt ctggaagggc
    29701 gcgtgaaacg tgtgacggat aaaccgctga tattgattaa taactatatt ggcgatccaa
    29761 aactcgacac tttatttaat caattaaccg ccgaacataa acactgatta cagaggtaaa
    29821 aatgaaaacc aaagttgctg ctatttatgg caagcgggat gtccgtctgc gcgaatttga
    29881 actgccagaa attaccgata atgaactgtt agtgagtgta atttctgaca gcgtctgttt
    29941 atcgacctgg aaagcggcgt tactcggtag tgaacataaa cgcgtacccg acgatttaga
    30001 aaatcatccg gtcattaccg ggcatgaatg tgccggggtt attgtcgaag tgggtaaaaa
    30061 tctcactggc aaatataaaa aaggccagcg ttttgtattg caaccggcga tggggttacc
    30121 aagcggatat tcagcaggct acagctacga atattttggc ggtaacgcca cctatatgat
    30181 tattcccgaa atcgccatta atttgggctg cgtattaccg tatcacggct cttattttgc
    30241 tgcggcgtcg ctggtagagc ctatgtgctg cattattggt gcttatcatg ccaattatca
    30301 caccacgcaa tatgtttatg agcatcgcat gggcgtcaaa cctggcggca atattgcact
    30361 gctggcgtgt gcgggtccga tgggcattgg cgctatcgat tacgccatta acggtggcat
    30421 acaaccatca cgggtggtgg tggtcgatat cgacgacaaa cgcctggcgc aggtgcagaa
    30481 gctgctgccg gtggatctgg cggccagtaa aggcattgag ctggtgtatg tgaataccaa
    30541 agggatgagc gatccggtcc agacgctgcg ggcgctgaca ggagatgtcg ggttcgatga
    30601 catttttgtt tatgcggcgg tgcctgctgt cgttgagatg gctgacgaat tactggcgga
    30661 agatggctgt ctgaacttct ttgccgggcc gacggataaa aacttcaaag tgccgtttaa
    30721 tttctacaac gtccattaca acagcacgca cgtagtcggt acatccggcg gttcaacgga
    30781 cgacatgaaa gaggcgattg cgttaagcgc cacggggcag ttgcagccgt cctttatggt
    30841 cacgcatatc ggtgggctgg atgcggtgcc agataccgtg ctcaatctgc cggatatccc
    30901 tggcggtaaa aaactcattt ataacggcgt gaccatgccg ctcactgcca ttgccgattt
    30961 tgccgaaaaa ggcaaaaccg atccgctatt taaagagttg gcgcggctgg ttgaggaaac
    31021 gcacggcatc tggaatgaac aggccgagaa atatctgctg gcacaatttg gcattgatat
    31081 cggggaggcc gcgcaatgat gtccctggcg tggccattat ttcgcgttac ggaacaggca
    31141 gcgttggctg cctggccgca aaccggatgt ggcgacaaaa ataaaattga tggcctggcg
    31201 gtcaccgcga tgcgccaggc attaaacgac gtcgcttttc gtggacgagt ggttatcggc
    31261 gaaggagaaa ttgaccatgc gccgatgctg tggattggcg aagaggtggg caaaggcgat
    31321 gggccagaag tcgatatcgc ggttgatccc attgaaggca cgcggatggt ggcgatgggg
    31381 cagagcaatg cgctggcggt catggctttc gcccctcggg atagcctgtt acatgcgccc
    31441 gatatgtata tgaaaaagct ggttgttaat cgactggctg ccggggtgat tgatctgtct
    31501 ttgcctctgg cggacaactt gcgtaacgtg gcgcgggcgt taggtaaacc gctggacaag
    31561 ctgcgcatgg ttacgctgga taaaccgcgc ctgagtgccg cgattgagga agcaacacag
    31621 ctgggcgtga aggtttttgc cctgccggat ggcgatgtcg ccgccagcgt gctgacctgc
    31681 tggcaggata atccttacga tgtgatgtac accatcggcg gtgcgccaga aggcgtgatt
    31741 tctgcctgtg ccgttaaagc attaggcggg gatatgcagg ccgagctgat tgacttttgt
    31801 caggctaaag gggattacac ggaaaatcgg cagattgcag agcaggagag caagcgttgc
    31861 aaggcaatgg gcgttgacgt taaccgtgtg tactcgctcg atgaactggt aagggggaac
    31921 gatatcctct ttagcgctac gggcgtgacg ggcggcgagt tggtgaacgg tatccaacag
    31981 acggcgaatg gggtgcggac gcagacatta ctgatcggcg gcgcggacca aacgtgtaat
    32041 ataatagact ccctgcattg atggtgatct atcggaagac caatggcgaa gctgacagaa
    32101 gatgatgtgc ttgagcaact ggatgcacag gacaatttat tttcatttat gaaaactgcg
    32161 cattctattt tgctccaggg gatacgccag tttctgccgt cgctgtttgt cgataacgat
    32221 gaagagatcg tggaatatgc agtgaagccg ttactcgccc aaagcggccc gcttgacgat
    32281 attgatgttg cgctgcgttt gatttatgcg ctggggaaaa tggataaatg gctgtacgcc
    32341 gatatcacgc atttttccca gttctggcat tacctgaacg aacaggatga aacgcccgga
    32401 tttgccgatg acatgacctg ggattttatc agcaacgtca atagcataac ccgcaatgcg
    32461 atgctctatg acgcgttgaa agcgatgaag ttcgccgatt tcgccgtctg gtcagaggcg
    32521 cgttttagcg ggatggtcaa aacggcgctg acgctggcag taacgacaat cttaaaggaa
    32581 ttaacgccgt gaaaattgaa ttaacggtga atgggctgaa gatacaggca cagtaccaga
    32641 acgaagagat tgaaaatgta cataaaccgt tgctgcgtat gctggcggcc ttacagacgg
    32701 taaatccgca gcggcgtacg gtggtttttc tttgcgctcc gccggggacg ggtaaatcta
    32761 ccctaaccac cttctgggaa tatctcgcgc aacaagaccc ggagctgccc gctattcaga
    32821 cgctcccgat ggatggtttt caccattaca atagctggct ggacgcgcat caattgcgcc
    32881 ccttcaaagg cgcaccagag acatttgacg ttgcgaaact ggcggaaaat ctgcgccagg
    32941 tggtggaagg ggattgtacg tggccgcagt acgatcgaca aaagcatgat cctgttgaag
    33001 atgcgttgca cgttaccgca ccactcgtca tcgtcgaagg aaactggttg ttgctggacg
    33061 acgagaagtg gtgccagctt gcgcaatttt gtgatttctc gatctttatc aacgcgccag
    33121 ctactgcgtt gcgggagcgt ctggtggggc gcaagctggc gggaggggta tcgctggcag
    33181 atgcagaagc cttttatgac cgtaccgacg ggccgaatgt tcgccgggtg ctggaagaga
    33241 gtctgcctgc aaatttgacc ttaatgatga ccgctacggg tgaatatcgt ctggtggatt
    33301 aacgttcctg attacaccat cttccagtcg caccacacga gagaaatgac gccgggtaaa
    33361 tgcggcgtca tgactgattg ctaccacgct cgtgcctcgc tggcggcact tctccagcca
    33421 gctttcaaac acgctcaacc aattttcgtc aaaatcccga ctcggctcgt ccagcagtaa
    33481 tagtggcgga gagagtgctt ccaggcaggc gacggcgacc atccgtcgct gcgcactatg
    33541 cagatccaac gggtgcgagt tggcagtgtc tgctaactga caacattgca gggcagcatg
    33601 ggtacgttga gtgatttcat ccgccgggca tttttgcagt ttcaaaccaa atgcgatctc
    33661 gtcagcgacg gtgctgtgaa aaagttggtt ttccgcttcc tgaaacagta cgccaacctt
    33721 tgccgcgcgc tggcggtttt tcaggttttt catcgcctgt tgttgcagca taaccgtacc
    33781 ggctgtgggc gtaagaagac ctgccattac tcgtaacagc gtcgatttcc ctgcgccatt
    33841 gtcaccggtc agcgccaacc attcaccttg tttgagttgc agagaaatgt cgcaaagaca
    33901 atccgttgcc gcgccaggcc agcgataaga tatctgattt aacgttaaca tagtggctgg
    33961 agcgctccgt tttgcaattg ccacgcttgc tggcagtagt tcaggaaagg tgtgcgatga
    34021 cgttcaaaaa gaataatcag cgaaccacgt tcaaacgccc agtgttgtaa gcgctgcaac
    34081 agcatttcgc tggcctgcgg cgtcaggcgg ctaaaggctt catcaagaat taagagcttt
    34141 ggctgcatcg cgatggcgca ggcgatgacc acgcgctgtg tctctccacc ggagagtgtt
    34201 gccgggtgac gatggcgcaa cggctggcac tcggtcagag tcagggcggc atcaatccgc
    34261 gccataatct ctgcttcagc aagacacaga ttttctggcc caaaggcaac ttcttcttcc
    34321 acgctaaagg tacagccgga gagctgcaaa tagggcgatt gctggacgag ctggatggtg
    34381 gcggattgtt cattaagcgg gagatgccca atgggcgtac cgaggagcgt ccccgtgcct
    34441 gttatctctc ctggcagaaa gtccggatac cagcccgcca tcagctgtgc cagcgtactt
    34501 ttgccactgc cgttgtcacc aaagatggcg accatccccg gtttgacata gtgaaagtca
    34561 taacagaacg gaggatgcgt tgagtgtgtc gggcaataac ggaactgttc taacgtaacc
    34621 atatccacgc tccgaattct gtcagcatca gcaagatcat ggtgtaacgc gctacgcgtt
    34681 gtagggtgct atcggctggt gcccacaacg ttgttcgtcg gttgtggatc cgaaatgccc
    34741 gcatatccag cgccgcacca cggacagcca gatcgttaag cgcattgtgt gttagcggaa
    34801 taattaacgc gggcatcgca cgtaaacgct gataccagcc ctcatccagc ggcacgccgc
    34861 gggcacgctg cgcttcatga ataatcgcta attgccgttt taactgctca acgaccaata
    34921 acggcccggc aaacagataa gcgacgcctg gcggcaggcg agaagcaaac agagcgcgaa
    34981 taaaacattg cacggggacg tactgcatcc acaactgtga ggttgaaaca atcgccagaa
    35041 tccgcaacca gagcgtaata gcatgcgacc accgttctgg cgagcgtggc gtgccgctta
    35101 gccattctgg tagccagcca ccgtgtacca gccacagccc ggctcccagc gagaacatca
    35161 gccagacgac atattttgcc cgccgccgcg tggcctttag cgcaataagg cagaaaaagg
    35221 tcgccgcgct atagatcggc aaaatggtct gcgcgggcag gataagcgtg gtgcaggccg
    35281 ccaatgccca cagtattaat gaggtaaatg gatgcattta gcgcacggca gccattgccg
    35341 gaaaatggcg cgtggtgcga atcggcagct ggcgcagtaa aagccagaca atcaccgctg
    35401 taagaatttt atcgaccagg ttcgcgccta tcaccgtaat cgccaccgac tccaccaggt
    35461 tttgccccat cgagtgcatc caggcaacga acaaatcggc tccgctaccg gtgacaccgc
    35521 cgaacagcgc agtacgcagc ggtacagcga cgaccgttac cgcaagcgta ataatcacgc
    35581 cgctgacaac gacttttggc agggtgcgaa accaacccgc tcgcgccagc caaccggcga
    35641 ccaggccgat gaccatcgcg acgggtgcaa atgcggcggc gatcgggtcg gtcagcaatc
    35701 cccagagtaa attggtcagt aagccggtca gcattccgat caccggacca agcagtaccg
    35761 cgctaataag tgtgccgatg gagtcgagaa atataggtaa tttgaccatg ctggccagtt
    35821 gcccgccgat catattgatg gcgatagata taacgatcaa caccagtgcc tgactggaaa
    35881 agtggctacg cgccataaga aaacctcttt gaaagtagtt atttaacggg ggaaatatgc
    35941 aactcgtcgt aaccagaaag cgcgcaaggt tggcggctaa acgccagttc ctcaaggcta
    36001 ccgatcacca gttcaagcgt ggtccgcacg gtacagccgg gcatcatatg accgccaagc
    36061 atcgtgccgt gtggatcgga aacgcagaga tgaagatgct caccactctg ttccagcgtg
    36121 ccgtttagtg agatcacttc gaatgtaccg ttcaggagcg tggtgccttc ttgcctggca
    36181 tagcgcaagg cgacgtcagt caggctaccg gtacagcctg caatccaggc ggcatgaagt
    36241 tgatgctgct gtgcaaaagc gtgtagctgg gaaaaaactt cctgcccggg gaggaggcgc
    36301 agtgcgtaaa agcgtgcgga ggaagaatat gggtagggca atgtcatcat tggagtactc
    36361 cgaaactcgg acgtattgcg gataatatcg acgctgcatc agtcatattc tgatataagt
    36421 cagggtttgt gacggcacaa cgaaaagatt ttgctacgct tattcgtgat tctgcaaagt
    36481 ggaataaatt ctacaggggc ggggctgaaa acgtgacgta agtcagataa ttgctttctt
    36541 tgactggaca atcattcctt ttattccacg tttcgcttat cctagctgaa gcgtttcagt
    36601 cgattaaatg ttcgacaatt aaccaatcag tcgcagtttg cgacaggtaa ggtttccccg
    36661 gacgatttgc tggattactc tgtctggcaa atttgctcag ggaaaacctt gcaggagatc
    36721 tatgaccgta cgcgtagcga taaatggctt cggtcgcatc gggcgtaatg tggttcgtgc
    36781 tttgtatgaa tccggacgcc gggcggaaat taccgtggtg gcaatcaacg aactggcgga
    36841 tgctgcgggc atggcgcatt tgttgaaata tgacaccagc catggccgtt ttgcatggga
    36901 agtacgacag gaacgcgatc aactttttgt tggtgatgac gccatccgcg tattgcatga
    36961 acgttcactg caatcgctcc cctggcgtga acttggcgtt gatgtagtcc tcgactgcac
    37021 cggcgtatat ggctcccgcg agcatggcga agcgcatatt gccgccgggg ccaaaaaagt
    37081 gctcttttca catcctggca gtaacgatct cgacgcgacc gttgtttacg gcgtcaatca
    37141 ggatcaactt cgtgcggaac accgcatcgt ttctaacgct tcctgtacca cgaattgcat
    37201 aattcccgtc atcaaattgt tagatgatgc gtacggtatt gagtccggca ctgtgaccac
    37261 aattcactcc gccatgcacg atcaacaggt tattgatgca taccatcctg acctgcgtcg
    37321 cacccgggca gccagccagt cgatcattcc ggtcgatact aaactggccg ccggtatcac
    37381 acgatttttt ccgcaattta acgatcgctt tgaagcgatt gcggtacgtg tgccaaccat
    37441 aaatgtgacg gcaatcgatt taagcgtgac ggtgaagaaa cctgtaaaag ccaatgaagt
    37501 caacctgttg ctgcaaaaag cagcacaagg tgcatttcat ggtatagttg actatacgga
    37561 attgccgttg gtctctgtag attttaacca cgatccgcac agtgccattg tcgatggcac
    37621 ccaaacccgg gtcagtggcg cacacctgat caaaacgttg gtctggtgcg ataacgaatg
    37681 gggctttgct aaccgaatgc tcgacacgac gttagctatg gctactgttg ctttcaggta
    37741 agacgcaagc agcgtctgca aaacttttag aatcaacgag aggattcacc atgtctgtaa
    37801 ttaagatgac cgatctggat cttgctggga aacgtgtatt tatccgtgcg gatctgaacg
    37861 taccagtaaa agacgggaaa gtaaccagcg acgcgcgtat ccgtgcttct ctgccgacca
    37921 tcgaactggc cctgaaacaa ggcgcaaaag tgatggtaac ttcccacctg ggtcgtccta
    37981 ccgaaggcga gtacaacgaa gaattctctc tgctgccggt tgttaactac ctgaaagaca
    38041 aactgtctaa cccggttcgt ctggttaaag attacctcga cggcgttgac gttgctgaag
    38101 gtgaactggt tgttctggaa aacgttcgct tcaacaaagg cgagaagaaa gacgacgaaa
    38161 ccctgtccaa aaaatacgct gcactgtgtg acgtgttcgt aatggacgca ttcggtactg
    38221 ctcaccgcgc gcaggcttct actcacggta tcggtaaatt cgctgacgtt gcgtgcgcag
    38281 gcccgctgct ggcagctgaa ctggacgcgc tgggtaaagc actgaaagaa cctgctcgcc
    38341 cgatggtggc tatcgttggt ggttctaaag tatctaccaa actgaccgtt ttggactccc
    38401 tgtctaaaat cgctgaccag ctgattgttg gtggtggtat cgctaacacc tttatcgcgg
    38461 cacaaggcca cgatgtgggt aaatccctgt acgaagctga cctggttgac gaagctaaac
    38521 gtctgctgac cacctgcaac atcccggttc cgtctgatgt tcgcgtagca accgagttct
    38581 ctgaaactgc accggctacc ctgaaatctg ttaacgatgt gaaagctgac gagcagatcc
    38641 tggatatcgg tgatgcttcc gctcaggaac tggctgaaat cctgaagaat gcgaaaacca
    38701 ttctgtggaa cggtccggtt ggcgtgttcg aattcccgaa cttccgcaaa ggtactgaaa
    38761 tcgtggctaa cgctatcgca gacagcgaag cgttctccat cgctggcggc ggcgacactc
    38821 tggcagcaat cgacctgttc ggcattgctg acaaaatctc ctacatctcc actggcggcg
    38881 gcgcattcct cgaattcgtg gaaggtaaag tactgcctgc agtagcgatg ctcgaagagc
    38941 gcgctaagaa gtaaaaaatc acagggcagg gaaacctgcc cttgtttcag cgcgctttta
    39001 gagcacgcat tttttcatac tctaaataat tcgagttgca ggaaggcgac aagagagtga
    39061 atccccagga gcttacataa gtaagtgact ggggtgagcg aacgcagacg cagcacatgc
    39121 aacttgaagt atgatgagta taaggcccga cgatacagga caagagacat gtctaagatt
    39181 tttgatttcg taaaacctgg cgtaatcact ggtgatgacg tacagaaagt tttccaggta
    39241 gcaaaagaaa acaacttcgc actgccagca gtaaactgcg tcggtactga ctccatcaac
    39301 gccgtactgg aaactgctgc taaagttaaa gcgccggtta tcgttcagtt ctccaacggt
    39361 ggtgcttcct ttatcgctgg taaaggcgtg aaatctgacg ttccgcaggg tgctgctatc
    39421 ctgggcgcga tctctggtgc gcatcacgtt caccagatgg ctgaacatta tggtgttccg
    39481 gttatcctgc acactgacca ctgcgcgaag aaactgctgc cgtggatcga cggtctgctg
    39541 gacgcgggtg aaaaacactt cgcagctacc ggtaagccgc tgttctcttc tcacatgatc
    39601 gacctgtctg aagaatctct gcaagagaac atcgaaatct gctctaaata cctggagcgt
    39661 atgtccaaaa tcggcatgac tctggaaatc gaactgggtt gcaccggtgg tgaagaagac
    39721 ggcgtggaca acagccacat ggacgcttct gcactgtaca cccagccgga agacgttgat
    39781 tacgcataca ccgagctgag caaaatcagc ccgcgtttca ccatcgctgc gtccttcggt
    39841 aacgtacacg gtgtttacaa gccgggtaac gtggttctga ctccgaccat cctgcgtgat
    39901 tctcaggaat atgtttctaa gaaacacaac ctgccgcaca acagcctgag cttcgtattc
    39961 cacggtggtt ccggttctac tgctcaggaa atcaaagact ccgtaagcta cggcgtagtg
    40021 aaaatgaaca tcgataccga tacccaatgg gcaacctggg aaggcgttct gaactactac
    40081 aaagcgaacg aagcttatct gcagggtcag ctgggtaacc cgaaaggcga agatcagccg
    40141 aacaagaaat actacgatcc gcgcgtatgg ctgcgtgccg gtcagacttc gatgatcgct
    40201 cgtctggaaa aagcattcca ggaactgaac gcgatcgacg ttctgtaaga tattcctttc
    40261 tgcttatctc aaggcccgct ctgcgggtct ttttttcgcc aaaagcaaaa ttgcctgatg
    40321 cgctacgctt atcaggccta cacgtatcct gcaatatatt gaatttgcag gtttttgtag
    40381 gccggataag gcgttcacgc cgcatccggc atgacaaagc gcacattgat atctgtctga
    40441 gccccgctct gcggggattt tttcgccaaa agcaaggtga ttcagatgag aaatctgtga
    40501 cctatttggc aaaattatgc tttattgttt acccttgtca gactgcccgt cataaggcgg
    40561 cggagtgtat ttctccattt tgagtcagtt gaaaaggaat attgaatgga agatttgaat
    40621 gttgtcgata gcataaacgg cgcgggaagc tggctggtag ctaaccaggc gctgctgcta
    40681 agttatgcag taaacatcgt ggcggcactc gcgatcatca tcgttggttt gattatcgcg
    40741 cggatgattt ccaacgcggt gaatcgcctg atgatctccc gtaaaatcga tgccactgtt
    40801 gctgattttc tttctgcatt agtccgttac ggtattatcg cctttacgct aatcgctgcc
    40861 ctgggacgcg tgggtgtaca aacagcgtca gtcattgctg tactcggtgc cgcaggctta
    40921 gctgttggtc tggctttgca ggggtcactt tctaacctgg ccgctggcgt gttacttgtc
    40981 atgttccgac cgttccgtgc cggagaatat gttgacctcg gcggcgtagc cggtactgtg
    41041 ctgagtgtgc agattttctc caccaccatg cgtactgcag acggtaaaat tatcgttatt
    41101 ccgaacggta aaattattgc cggaaatatt attaacttct cccgcgagcc agttcgccgt
    41161 aacgaattta ttattggcgt ggcgtatgat tccgatatcg atcaggttaa gcagatcctg
    41221 accaatatta tccagtctga agatcgcatt ttgaaagatc gcgaaatgac tgtgcgcctg
    41281 aacgaacttg gtgcatcgtc gattaatttc gtggtccgcg tctggagcaa cagcggcgat
    41341 ctgcaaaacg tgtactggga tgtgctggag cgtattaaac gtgaatttga tgccgccggt
    41401 atcagcttcc cgtacccgca aatggatgtg aactttaagc gggtgaaaga agacaaagct
    41461 gcgtaatcaa cgctgaggcc agataatact ctctggcctc tctcttatta gttattctga
    41521 ttgccaatta atattatcaa tttccgctaa taacaatccc gcgatatagt ctctgcatca
    41581 gatacttaat tcggaatatc caacgtgttt tcttattact ttcaaggtct tgatcttggg
    41641 gc
//
LOCUS       DAJTMC010000012        40625 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.012, whole genome shotgun sequence.
ACCESSION   DAJTMC010000012 DAJTMC010000000
VERSION     DAJTMC010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40625
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.012"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(265..1122)
                     /gene="murI"
                     /locus_tag="OO130_000597"
     CDS             complement(265..1122)
                     /gene="murI"
                     /locus_tag="OO130_000597"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCR5364119.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
     gene            complement(1067..2915)
                     /gene="btuB"
                     /locus_tag="OO130_000598"
                     /pseudo
     CDS             complement(1067..2915)
                     /gene="btuB"
                     /locus_tag="OO130_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418401.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
     regulatory      complement(2969..3159)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            3273..4373
                     /gene="trmA"
                     /locus_tag="OO130_000599"
     CDS             3273..4373
                     /gene="trmA"
                     /locus_tag="OO130_000599"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HCR5364120.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IELDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPCILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     gene            complement(4413..4772)
                     /locus_tag="OO130_000600"
     CDS             complement(4413..4772)
                     /locus_tag="OO130_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HCR5364121.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(4772..5422)
                     /gene="fabR"
                     /locus_tag="OO130_000601"
     CDS             complement(4772..5422)
                     /gene="fabR"
                     /locus_tag="OO130_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HCR5364122.1"
                     /translation="MMGIRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            5743..7143
                     /gene="sthA"
                     /locus_tag="OO130_000602"
     CDS             5743..7143
                     /gene="sthA"
                     /locus_tag="OO130_000602"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HCR5364123.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLVLDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            complement(7126..8043)
                     /gene="oxyR"
                     /locus_tag="OO130_000603"
     CDS             complement(7126..8043)
                     /gene="oxyR"
                     /locus_tag="OO130_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HCR5364124.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(8310..9683)
                     /gene="argH"
                     /locus_tag="OO130_000604"
     CDS             complement(8310..9683)
                     /gene="argH"
                     /locus_tag="OO130_000604"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HCR5364125.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDAFDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLDELQKFSHVIGEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FAQARLG"
     gene            complement(9744..10520)
                     /gene="argB"
                     /locus_tag="OO130_000605"
     CDS             complement(9744..10520)
                     /gene="argB"
                     /locus_tag="OO130_000605"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HCR5364126.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            complement(10528..11532)
                     /gene="argC"
                     /locus_tag="OO130_000606"
     CDS             complement(10528..11532)
                     /gene="argC"
                     /locus_tag="OO130_000606"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HCR5364127.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPAINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQVLQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            11686..12837
                     /gene="argE"
                     /locus_tag="OO130_000607"
     CDS             11686..12837
                     /gene="argE"
                     /locus_tag="OO130_000607"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HCR5364128.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASIGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDIDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPGMTLNELNG
                     LLNDALAPVIERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            13189..15840
                     /gene="ppc"
                     /locus_tag="OO130_000608"
     CDS             13189..15840
                     /gene="ppc"
                     /locus_tag="OO130_000608"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HCR5364129.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEAPPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYKSWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDVYRGYVRENKDFVPYL
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            16023..17756
                     /locus_tag="OO130_000609"
     CDS             16023..17756
                     /locus_tag="OO130_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HCR5364130.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            17971..18822
                     /locus_tag="OO130_000610"
     CDS             17971..18822
                     /locus_tag="OO130_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5364131.1"
                     /translation="MYHDISYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(18809..19150)
                     /locus_tag="OO130_000611"
     CDS             complement(18809..19150)
                     /locus_tag="OO130_000611"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HCR5364132.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(19152..20030)
                     /locus_tag="OO130_000612"
     CDS             complement(19152..20030)
                     /locus_tag="OO130_000612"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418387.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HCR5364133.1"
                     /translation="MTSSAGQRISCNVVETRLNDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLNIRQIHLLPFHQYGEPKYRLLGKTWSMKDVAAPSSADVAT
                     MREMAERAGFQVTVGG"
     gene            complement(19996..22293)
                     /locus_tag="OO130_000613"
     CDS             complement(19996..22293)
                     /locus_tag="OO130_000613"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCR5364134.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAVLAETMAANCT
                     DAQRREELLTIAEISRHNAEHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTPGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDEQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNADTLWEAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            complement(22344..22664)
                     /locus_tag="OO130_000614"
     CDS             complement(22344..22664)
                     /locus_tag="OO130_000614"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HCR5364135.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            complement(22679..23758)
                     /locus_tag="OO130_000615"
     CDS             complement(22679..23758)
                     /locus_tag="OO130_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HCR5364136.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVVI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            24067..26568
                     /gene="ptsP"
                     /locus_tag="OO130_000616"
     CDS             24067..26568
                     /gene="ptsP"
                     /locus_tag="OO130_000616"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR5364137.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLAGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALSNLPAAKDVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSSILEAHRSLAGDTSLRQHLLTGVSEGLSCAEAIVAS
                     ANHFCEEFARSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQPAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMTQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            26580..27242
                     /gene="fsa"
                     /locus_tag="OO130_000617"
     CDS             26580..27242
                     /gene="fsa"
                     /locus_tag="OO130_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HCR5364138.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKMLKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            27253..28356
                     /gene="gldA"
                     /locus_tag="OO130_000618"
     CDS             27253..28356
                     /gene="gldA"
                     /locus_tag="OO130_000618"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HCR5364139.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAAFSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAEGE
                     TIHNMPGGATPDQVYATLLVADQYGQRFLQEWE"
     gene            28630..29247
                     /locus_tag="OO130_000619"
     CDS             28630..29247
                     /locus_tag="OO130_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HCR5364140.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGNTLFYDPAYVRLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWKLKRPDSNIDHRRVPNLETWFSRHNKTRPISKNAGDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(29274..30179)
                     /gene="yijE"
                     /locus_tag="OO130_000620"
     CDS             complement(29274..30179)
                     /gene="yijE"
                     /locus_tag="OO130_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HCR5364141.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMHPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYTRHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(30273..32453)
                     /gene="katG"
                     /locus_tag="OO130_000621"
     CDS             complement(30273..32453)
                     /gene="katG"
                     /locus_tag="OO130_000621"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HCR5364142.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     SSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(32782..33672)
                     /gene="metF"
                     /locus_tag="OO130_000622"
     CDS             complement(32782..33672)
                     /gene="metF"
                     /locus_tag="OO130_000622"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HCR5364143.1"
                     /translation="MSFFHASQRDALNQRLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            complement(34021..36453)
                     /gene="metL"
                     /locus_tag="OO130_000623"
     CDS             complement(34021..36453)
                     /gene="metL"
                     /locus_tag="OO130_000623"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HCR5364144.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(36456..37616)
                     /gene="metB"
                     /locus_tag="OO130_000624"
     CDS             complement(36456..37616)
                     /gene="metB"
                     /locus_tag="OO130_000624"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HCR5364145.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWTNNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            37893..38210
                     /gene="metJ"
                     /locus_tag="OO130_000625"
     CDS             37893..38210
                     /gene="metJ"
                     /locus_tag="OO130_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HCR5364146.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            38394..39002
                     /locus_tag="OO130_000626"
     CDS             38394..39002
                     /locus_tag="OO130_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HCR5364147.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVIRNKKPYVFEAVGPVKYTPLKQWIAHGEKGKYVVRRVEGGLSVEQQQK
                     LAQTVKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            39147..39822
                     /locus_tag="OO130_000627"
                     /pseudo
     CDS             39147..39822
                     /locus_tag="OO130_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(39994..40266)
                     /locus_tag="OO130_000628"
     CDS             complement(39994..40266)
                     /locus_tag="OO130_000628"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5364148.1"
                     /translation="MELQLSDKGVIKMKAIQCCYFLALVSSNLMAGVMGNDNIFLSNS
                     DSAALYCVAKIFPDGENILIYKVNIINASSKKDVFFMKAQHQPTMT"
     gene            complement(40232..40625)
                     /locus_tag="OO130_000629"
                     /pseudo
     CDS             complement(40232..40625)
                     /locus_tag="OO130_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747579.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4329 domain-containing protein"
BASE COUNT         9991 a        11161 c        10102 g         9371 t
ORIGIN      
        1 cccacacaga ttgtctgata aattgttaaa gagcagtgcc gcttcgcttt ttctcagcgg
       61 cgcggggtgt gcataatacg ctttcccgct acagagtcaa gcatttattt ttgctttctc
      121 tgtcggaatt ctcaggagaa ccccgctgac ccggcggcgt gtttgccgtt gttccgtgtc
      181 agtggtggcg cattataggg agttattccg gcctgacaag aggaaattta aaataatttt
      241 ctgaccgcgc aacattcaac caaatcagcc taaaactgcc agtttttcga gcgtttcgaa
      301 gccgtaacgc tgtaaaacgg gcaataattg ttcagcttct ggcgtcattg ccatacaaaa
      361 ggcaatattc gcatcggcag attttgcatc cggggcttca tgttctaaca accaggccgt
      421 tcggcgagca atcgctgcgc cagaatccac tagccgggtt ccctctggca gcacttgtaa
      481 cagttcttct tgtagtagag ggaaatgggt gcaacccaat acaacggtat ctggcggctc
      541 tttcattctt aaccacgggc gtaggatacg ttttagtgca tccagagaaa catcttcgcc
      601 atgtagcttc gcttcagcca actcaaccat ctctgccgag cccagcattt ctatctggca
      661 ttcattagcg aaacgcgcga tcagctcatg agtataagaa cgtttaactg ttccgcgggt
      721 tgccagtaat ccgacaatgc catttgccgt cagacgtgca gcaggtttaa tcgccggcac
      781 gacaccaaca accgggaagt cgaacttttc gcgtaatgca ggaagtgaaa cggtactggc
      841 agtgttgcaa gcgaccacag ccagcgcaag gggataacgt tcttgcaccg cggtgacaat
      901 tgccaccact cgctcaacaa taaacgcttc gcttttttcg ccatacggga aagcgacgtt
      961 atcgaaagca taaatgtaat ggagatccgg taagagatgc cggatctcgt catagaccga
     1021 caacccaccg acgccggagt caaacaccag cacggtggga cgtggttcag aaggtgtagc
     1081 tgccagacaa ggtgtattcc cgtcctgcag tttggtagcc atagactgtc tcataatctt
     1141 tgtcgaacag gttggctatt ttaccacgaa ctgtcaggtg agaggtgacc ggatacgcaa
     1201 ccgcaagatc ccacaagctc acaccgccca ttttaacggt ttgataagga taagatgagt
     1261 aatccttatc atagcgagtg cctaaatact gataagtaat accccagtcg aagtcataca
     1321 actgccagtc gagctggtat ttcacctgct gtttagcacg gcgtaacaac ggcgtgtcgg
     1381 taatcgcatt gcgcgcatcg acataatcat aactcacagt atgcgtcagt ggtccggtat
     1441 caaaattggc ggtcgcctcg acgcccttaa tccgcgcttt cccttcgttg taatatttca
     1501 gggtgtgatc atcataatcg atcaagtcac tgacatcgtt acgatatccg gaaatacgcc
     1561 agttcacccc agcggttaag ccttcaaacg cgccttccca ctgtttgctt ttctccgggt
     1621 ccagattcgg atttccgtag aagccataca gttgccccag atttggtgcc ttataagatg
     1681 tcccgtagga agcaatgaag cgataacctt cgatgaattc ccaaccggcg ctggtttgcc
     1741 aggttccatg acgaccaaac tgtgagttat cgtcactgcg tgctgcgcct tcaaaggtaa
     1801 aatcgccgac ttgttgcagc ccggtcagat agatgccggt attacgttga tcatatccat
     1861 cctcaacata acctgtaccc ggcgtcgtag tctgtttctg ccagtcgaca cccgcaccaa
     1921 tactaccgtg accaacgatg acattgtttg cccactggac ggtgtattgc ttcatctcat
     1981 cgagcgtcgc cgacgaatca taacgaccat aatggggatc gtagttgtaa tctttgctat
     2041 ggctatagct ggtaatgagt tgtgatttaa tcagttcgcc gttatagcgc agcccggcgt
     2101 cccaactttg gctatagagt ttacggatat cgagcaacgg tgaaccggga gaataatacg
     2161 cgtcataatt ggtacggtta tcatagccat agccgcgcac aaagccgctc caggcatcag
     2221 taaagttatg ctccagcgcg ccataaagcg ttttacttaa aaaaacatcg ttatctgtct
     2281 gcgcttgcgt tccggtatta ccataggcaa caacatcata accatgagta tgggcataat
     2341 cgcccaacag cgttacccgt gtcttatccc ccagttgttg ctgcgtagag acatcatagt
     2401 tctgataact attgcttccc caccctgctg aaatttccgt tccggggtca tcgcgcgtcg
     2461 tgatgatatt caccaccccg cctattgcat cggaaccata aacagcggag cgcggcccac
     2521 ggatatattc aacacgctgg acaagcgcac gcaataggga actggctaag gtcggcagaa
     2581 ccactcaccc ccgccagatt caggcgtacg ccatcaatta acaccaacac atgactggca
     2641 tttgtaccgc gaataaaaat agatgagagc tgacctgaac cgccgttttg ggtgatatcg
     2701 acgcccggaa gacggcgcag cacatcattg accgaggtcg actgccagcg gtcgatatcc
     2761 tgacgggtca caacggtggt tggtgcaagc acagtgctgc gcggctgttc aaaacggtta
     2821 gcagtaacga cgagagtatc cgggctggta tcctgtgccc aagcggaaaa ggctgtgaca
     2881 gaacacgccg tcagcagcga agctttttta atcattgtaa agcatccaca atagaagaag
     2941 gatgccgcag gtttcatcaa tattacgcga tgatgagacg ttggccggca ggtcttcggg
     3001 cttggagggg tatctaagat actaagagat gatgacttcc caccggatgg cagtgtccgc
     3061 ataacgcaat catcgcacct ttccttaccg ctgcgcgtca gctccagatt cgcactggat
     3121 tccctattaa ctcacaggac cggcaagtgg atgctacagg ttgtaacaag ttactgtcca
     3181 gacgtagctc acaaatagga attcatcaag atctggacat ctgatgagca atccctacaa
     3241 tcgccgcgta ctttaatttt tcaggataca tcatgacccc cgaacacctt ccaacagaac
     3301 agtatgaagc gcagttagcc gaaaaagtgg tacgtttgca aagtatgatg gcaccgtttt
     3361 ctgacctggt tccggaagtg tttcgctcgc cggtcagtca ttaccggatg cgtgcggagt
     3421 tccgcatctg gcacgatggc gatgacctgt atcacatcat tttcgatcaa caaaccaaaa
     3481 gccgtatccg cgtggatagc ttccccgccg ccagtgaact tatcaaccag ttgatgacgg
     3541 cgatgattgc gggtgtgcgt aataatcccg ttctgcgcca caagttgttc cagattgatt
     3601 acctcaccac gctgagtaat caggcggtgg tttccctgct ataccataag aagctggatg
     3661 atgagtggcg tcaggaagcg gaggccctgc gcgatgcact gcgcgcgcag aatctgaatg
     3721 tgcatctgat tggtcgggca acgaaaacca aaatcgagct ggatcaagat tacatcgacg
     3781 agcgtctgcc ggtcgcaggg aaagagatga tctaccgtca ggtagaaaac agctttaccc
     3841 agccgaacgc ggcgatgaat attcagatgc tggaatgggc gctggacgta accaaaggct
     3901 caaaaggcga tttactggag ctgtactgcg gcaacggtaa cttttcatta gcgctggcgc
     3961 gcaattttga tcgggtatta gccaccgaaa tcgctaagcc gtcggttgct gctgcgcaat
     4021 acaacatcgc agctaaccat attgataacg tacaaattat tcgtatggcg gcagaagaat
     4081 ttactcaggc gatgaatggt gtgcgcgagt ttaaccgcct gcaagggatc gacttaaaga
     4141 gttatcagtg cgaaaccatt tttgtcgacc caccgcgcag cggtctggac agtgaaaccg
     4201 agaaaatggt gcaggcgtat ccgtgtattt tgtacatctc ctgtaacccg gaaacgttat
     4261 gcaagaatct ggaaacatta agccagacgc acaaggtcga acgtctggct ctgtttgatc
     4321 agttccccta cacgcaccat atggagtgcg gcgtattact gaccgcgaag taaaacccat
     4381 gccggatgcg ccagcatccg gcataatacc gattactcag caacttcctg cttacggtta
     4441 cgcatcttcg cgccaatcca gaacaccata atgacggaga gtactgccgg gaagaagtta
     4501 gagccgatat ccggatattc cgcacgaact accgtgctgt acagcagtac gccgagaata
     4561 aaacaggcag ctgccagacc cggcaagcct accggcatag tgcgattaag ataacgttga
     4621 tgcaggcagt aaaccgtcag caccagggaa ataatcggga atacagaaaa tggcacaatg
     4681 gagctaaaca gcgctgcgaa agtaccatta atcgataagc cagcaactaa cgccagcagc
     4741 agcgtacctc tatcttgatt tgcttgtttc attactcgtc cttcacattt cccggaataa
     4801 ttgcggtttt ctcttgttca cggcgatacc agtaataagc ccctttcgaa atcattcgca
     4861 gttgcagtac cagtcgctct tctaattgcc gacgttgttc gacgccgacg tccaacgcct
     4921 cggcacccgc actgaagaca attgtcacca ttgcttcggc ttgcgcttca gtaaacgcac
     4981 gcggcatatg gttttcgagt tccagatagt ccgcaagttc cgcaatgaag tgctgaattt
     5041 cacgcgcaac ggcggcacga aacgcagcgg aggtgccgga gcgttcccgc aataataacc
     5101 ggaaggcgtt aggattatta ccgatgaact ccataaatgt ggagaccgag gtgcggatca
     5161 cactcccgcc tttggcgata cgctgacgcg cctggcgcat gagttggcgt agcattaaac
     5221 cgctctcatc aaccatggtc agacccagtt cgtctacgtc gcggaaatgc cgataaaaag
     5281 aggtgggagc aatgcccgct tcacgcgcca cttcacgcaa actcaggctg gcgaagctgc
     5341 gttcagcact taattggcta aatgcggctt ccaccagcga acggcgggtt ttttcttttt
     5401 gttgcgctct tatgcccatc acgatgtctg aatccttgcc aaaagtactg ctggcactat
     5461 accagagaat gaacatactg gattgctcca gtattgtgaa tgaacggtaa cgcaataaaa
     5521 catatttact ggccggttct tttacgtaca gcggaaacct gccgcttaaa cggagagtat
     5581 cgtcgataaa aatccaataa aacgtcaggg caaaagtaag aaacagacaa agcaaaggcc
     5641 gctcaggata tagccagata aatgacgggg atcaattggc ttacccgcga taaaatgtta
     5701 ccattctgtt gcttttatgt ataagaacag gtaagcccta ccatgccaca ttcctacgat
     5761 tacgatgcca tagtaatagg ttccggcccc ggcggcgaag gcgctgcaat gggcctggtt
     5821 aagcaaggtg cgcgcgtcgc agttatcgag cgttatcaaa atgttggcgg cggttgcacc
     5881 cactggggca ccatcccgtc gaaagctctc cgtcacgccg tcagccgcat tatagaattc
     5941 aatcaaaacc cactatacag cgaccattcc cgactgctcc gctcttcttt tgccgatatc
     6001 cttaaccatg ccgataacgt gattaatcaa caaacgcgca tgcgtcaggg attttacgaa
     6061 cgtaatcact gtgaaatatt gcaggggaac gctcgctttg ttgacgagca tacgttggtg
     6121 ctggattgcc cggacggcag cgtcgaaaca ctaaccgctg aaaaatttgt tattgcctgc
     6181 ggctctcgtc catatcatcc aacagatgtt gatttcaccc atccacgcat ttacgacagc
     6241 gactcaattc ttagcatgca ccacgaaccg cgccatgtac ttatctatgg tgctggagtg
     6301 atcggctgtg aatatgcgtc gatcttccgc ggtatggatg taaaagtgga tctgatcaac
     6361 acccgcgatc gcctgctggc atttctcgat caagagatgt cagattctct ctcctatcac
     6421 ttctggaaca gtggcgtagt gattcgtcac aacgaagagt acgagaagat cgaaggctgt
     6481 gacgatggtg tgatcatgca tttgaagtcg ggtaaaaaac tgaaagctga ctgcctgctc
     6541 tatgccaacg gtcgcaccgg taataccgat tcgctggcgt tacagaacat tgggctggaa
     6601 actgacagcc gcggacagct gaaggtcaac agcatgtatc agaccgcaca gccgcacgtt
     6661 tacgcggtgg gcgacgtgat tggttatccg agcctggcat cagcggccta tgaccagggg
     6721 cgcattgccg cgcaggcgct ggtaaaaggc gaagccaccg cacatctgat tgaagatatc
     6781 cctaccggca tttacaccat cccggaaatc agctctgtgg gcaaaaccga acagcagctg
     6841 accgcaatga aagtgccata tgaagtgggc cgcgcccagt ttaaacatct ggcacgcgca
     6901 caaatcgtcg gcatgaacgt gggcacgctg aaaattttgt tccatcggga aacaaaagag
     6961 attctgggta ttcactgctt tggcgagcgc gctgccgaaa ttattcatat cggtcaggcg
     7021 attatggaac agaaaggtgg cggcaacact attgagtact tcgtcaacac cacctttaac
     7081 tacccgacga tggcagaagc ctatcgggta gctgcgctaa atggcttaaa ccgcctgttt
     7141 taacacttta tcgaaatggc catccattct tgcgcggatg gcctctgcca gctgctcata
     7201 gcggctgcgc agcggtgagc caggacgata aaccaggcca atcgtgcggc gaggttccgg
     7261 cttaatacac ggcaaataaa caaccccatc gcgtttgcgc tccggcggca cagccagcgc
     7321 aggcagtaaa gtgatcccgc tacctgccgc caccatgtta cgcagtgtct ccaggctggt
     7381 cgcgcggaag tgtgtatctt catccgcccc ggcttcaaag cagaaaccca ttgcctgatc
     7441 gcgcaaacag tgaccatctt ccagcatcag cagtttttcc cctgccagat cggccatcgg
     7501 tacgcattcg cggttcgccc acgggtgatc ttcatagata gccagcaaca ttggctcatc
     7561 aaacaacggc acttcaatga atgcttcgct ctctttcacc agcgcgagga tcacgcaatc
     7621 gagtttgccg ctgtccagtt gcgccagtaa ctggtgggtc tgtgcttcat gcagatacat
     7681 ttccagcttt ggaaatgtct ggtgcagcat cgggataata tgcggcagca ggtacggtcc
     7741 aaccgtagga atcaaaccaa tatgcagcgg tccggacatc gtctcgccct gctggcttgc
     7801 catctcttta aggactttca cctcacgcag cacggtacgc gcctgatcca ccagcagcat
     7861 tcccgcctgg gtgaacaaca ctttacggct ggtccgctcc agcaacatca cgcccagctc
     7921 atcttccagc ttacgaattt ggccgctaag cgtcggctgg ctaacgtggc aggaatctgc
     7981 cgcacgccga aaatggcggt gttcagccaa tgccaccagg tactcaagat cacgaatatt
     8041 cattatccat cctccatcgc cacgatagtt catggcgata ggtagaatag caatgaacga
     8101 ttatccctat caagcattct gactgataat tgctcacaga aacggagcgg cacctctttt
     8161 aacccttgaa gtcactgccc gtttcgagag tttctcaact cgaataacta aagccaacgt
     8221 gaacttttgc ggatctccag gatccgcttt tttttgccat aaaaaagccc ggcgataagc
     8281 caggctcaaa tttgtacata taaatgttct taccccaacc gcgcctgcgc aaaagcaatc
     8341 gcttgggcca cctgctgcgg tgagacgccg ccttttgccg cacgcttgtc gaggcacgat
     8401 tgtagcgaca gaatcggata gacatcttcg ccaatcacgt ggctgaattt ctgcaactcg
     8461 tcgagcggca gatcttccag cggtttgccc tgacgaatgg cttccaccac cgcttcaccc
     8521 acgatatggt gcgcctcgcg gaaaggtacg cctttcgcca ccaggtaatc cgccagttcg
     8581 gtggcgttgg cgtaaccctg ctgagccgct tcctggcaac gtggacgttt cacctgaatg
     8641 ccgtccagca ccagcgccgc catatgcagg cagtccagcc aggtatcgaa cgcgtcgaac
     8701 agaccttctt tgtcttcctg catatctttg ttgtaagcca gcggcaaacc tttcagcgtc
     8761 atcatcatgc cggttaacgc accctgcacc cgaccgcatt taccgcgaat cagctccagc
     8821 gcatccgggt ttttcttctg cggcattaat gatgaaccgg aagtcacgcg gtcagaaagc
     8881 tccacaaacc ccgcttcgcc ggtgttaaag aaaatcagat cttcagcaaa acgcgacaga
     8941 tgcaccatgc cgatagcggc agccgacagc agttccaaca catggtcacg gtcagaaacg
     9001 ctgtccagac tgttacgggt cgccgaagca aagcccagcc agcctgctaa ctgttcacgg
     9061 tcgatttcat aggccgttcc cgccagcgcg ccacagccta atgggctgac atccagacgc
     9121 ttaagcgcat cctgcaaacg gctttcatca cgcgccagca tctcaacata ggccaggcac
     9181 cagtgcgcga acgtcaccgg ctgggcgcgt tgcaggtgag tgtaacctgg cattaccgcg
     9241 tcctgattgt tttgtgcggt ttccaccagc gccgattgca gctgccggtt agccgtcagt
     9301 aactcgctaa cggtatcttt gcaccacagt ttcaggtcag tcgctacctg atcattacgg
     9361 ctacgcccgg tatgcagctt tttgcctaac tggcccactt tgtcgatcag tttgccttcc
     9421 acccagctat ggatatcttc ggcgtcgctt tcaaggattt gttgtggcct ggcgcgaaca
     9481 tcttccagca acacgttcag cgcctcttcc agttgcgcct gctcttctgc ggttaacacg
     9541 ccgaccgtta ccagggcttt ggaccaggcc acagagccaa caatatcctg ctccgccaga
     9601 cggtaatcaa agcgcagtga gtcgttgaat tgtttgaacc gttgatctgc tgcctgggta
     9661 aaacgcccgc cccaaagtgc cataactctg tttccttatt ttgaaattca atgccggaaa
     9721 gctgcgcctc cggctaacaa aacttaagct aaaatccgcg tacccatcgg cataccgtta
     9781 aacagtgccg gaagctgctc cgcatgacgc caggaggcga tatctaccgg acggcccagc
     9841 gtgcgggccg catccagcgc cgcgttcact ttcactatca tgccgtcagt aataatgccc
     9901 tgctcaatca gttgttctgc tttcgcggcg gtcatttcgg caatgcgttg ccctttgccg
     9961 tcgagaatgc cgctgacgtc ggagagcaaa atcagatccg cgcccagcgt tgccgccagc
    10021 gccgttgccg cctggtcggc attgacgttc atcagttgcc cttcgtctgt tacgccaatg
    10081 gagctgacca ccggcagata accgttctcc agcaaggagt tgataagctt aggcgaacct
    10141 ggctgcgcca gtccaacatg acctaactct tcatcaagct gggtcacttt gacgctgtcg
    10201 ccatcaccga gaaacaaacc tacagccgca atctgatgtt tcttcgccca tgccaacagg
    10261 gttttatttg ccgttcccgc cagtgctccg gtgataatgt ctatctgatc agcaggcgtc
    10321 acccgcaggc cgtttttctt tttcaccggc agattcagcc ctttcatcag ctcatccacc
    10381 acgcaaccgc cgccgtgcac aatcaccagc ggacgctgat gtgactcacg ataattcacc
    10441 agtgcgctaa acagacgttc cagcgcctct tcactatcca gcagtacgcc gcccagtttg
    10501 ataattaatg gattcatcat tgcaccctta aataagagac tgcgtttccg catagccgaa
    10561 acgaatattg gcgcactgta ccgcttgtgc cgccgcgcct ttcagtaagt tgtcttcggt
    10621 cgccacaata atcagatgct cgccctgaac ggcaaacccg atatcgcaaa atggcagccc
    10681 aacgacattt ttcagcgccg gaacgccttt gtcatacagc cgcaccagcg gtttatgggc
    10741 atacgcctgc tgtaacactt gcgcgacttg cgcctgggtc acacccgatt tcaggcggca
    10801 ggtaatggtt tcgagaatgc cgcgcgggaa attgcccaga tgtggggtga agataacgtc
    10861 agcaccgagg tgtgtggcga tctctggttg atggcgatga gtaaagacgc catacggttg
    10921 caggctaact tcacaaaaac tatttgaaat cgctgcctta cgtcctgcac cgctcacgcc
    10981 gctggtggcg ttaatcgccg gccactgatt gaggtcaaga agatcggcat caatcaacgg
    11041 tttcagcgcc agctgtgccg ccgtcggata acagcctggc accgcaatca aattcgcttc
    11101 ttttaattta ttaccgcacc actccgccag accgtaggct gcctgttcca gcagttccgg
    11161 gtattgatgg gtaaagccgt aatatttttc atagaaggtg gcgtcgttaa cacgaaacgc
    11221 gccggaaagg tcgaacacca cgcagcccgc ttcaagaaac tgcggcgcta aatcgtggct
    11281 aacttcatgg gcggtggcga gaaacactac gtccacccct gggctaaact cgctgatatc
    11341 cgacatcggc tgcaacggca gctcaacgat gccttttagc tgcggatgca aatcggagat
    11401 taactttccc gcatcattgc tttgcgctga aacagtcaaa gcggttatgt tcatatgcgg
    11461 atggcgattt acataggtca ctagctctgc gccagcgtag ccgctggcac ccacaatcag
    11521 cgtattcaac atcggggcta ttcaccttct tatgtctggt tgccaggtta aacgtaaaac
    11581 attcacctta cggctggtgg gttttattac gctcaacgtt agtgtatttt tattcataaa
    11641 tactgcatga atattgatac tatcatgacc agaggtgtgt caacaatgaa aaacaaatta
    11701 ccgccattta tcgagattta ccgcgctctg attgccacac cttcaataag cgctacggaa
    11761 gaggcactcg atcaaagcaa tgcagattta atcactctgc tggcggactg gtttaaagat
    11821 ttgggcttca atgtggaagt gcagcctgtt ccaggaactc gcaacaaatt caatatgctg
    11881 gcaagtatcg gacagggggc tggcggcttg ttgctggcgg ggcataccga tacggtgcca
    11941 tttgatgacg gtcgctggac gcgcgatccg tttacactga cggagcatga cggcaagctt
    12001 tacggcttag gcaccgccga catgaaaggc ttttttgcgt ttatccttga tgcgctacgc
    12061 gatatcgacg tcacgaaact gaaaaaaccg ctctacattc tggcgactgc tgatgaagaa
    12121 accagtatgg ccggagcgcg ttattttgcc gaaactaccg ccctgcgccc ggattgcgcc
    12181 atcattggcg aaccgacgtc actacaaccg gtacgcgcac ataaaggtca tatctctaac
    12241 gccatccgta ttcagggtca gtcggggcac tccagcgatc cagcacgcgg agttaacgct
    12301 atcgaactaa tgcacgacgc catcgggcat attttgcaat tgcgcgataa cctgaaagaa
    12361 cgttatcact acgaagcatt taccgtgcca taccctacgc tcaacctcgg gcatattcac
    12421 ggtggcgacg cttctaaccg tatttgcgct tgctgtgagt tgcatatgga tattcgtccg
    12481 ctgcctggca tgacactcaa tgaacttaat ggtttgctca acgatgcatt ggctccggtg
    12541 atcgaacgct ggccgggtcg tctgacggtc gacgagctgc atccgccgat ccctggctat
    12601 gaatgcccac cgaatcatca actggttgaa gtggttgaga aattgctcgg agcaaaaacc
    12661 gaagtggtga attactgtac cgaagcgccg tttattcaaa cgttatgccc gacgctggtg
    12721 ctcggtccgg gttccatcaa tcaagctcat cagcctgatg aatatctgga gacgcggttt
    12781 attaaaccca cccgtgagtt gattatccag gtaattcacc atttttgctg gcattaaaac
    12841 gtaggccgga taaggcgctc gcgccgcatc cggcaccgtt gccaaactcc ggtgccgcaa
    12901 taatgtcgga tgcgatgctt gcgcatctta tccgacctac acctttggtg ttatgtatgg
    12961 cgatttttta acatttccat aagttacgct tatttaaagc gtcgtgaatt taatggcgta
    13021 aattcctgct atttattcgt ttgctgaagc gatttcgcag catttgacgt caccgctttt
    13081 acgtggcttt ataaaagacg acgaaaagca aagcccgagc atattcgcgc caatgcgacg
    13141 tgaaggatac agggctatca aacgataaga tggggtgtct ggggtaatat gaacgaacaa
    13201 tattccgcat tgcgtagtaa tgtcagtatg ctcggcaaag tgctgggaga aaccatcaag
    13261 gatgcgttgg gagaacacat tcttgaacgc gtagaaacta tccgtaagtt gtccaaatct
    13321 tcacgcgctg gcaatgatgc taaccgccag gagttactca ccaccttaca aaatttgtcg
    13381 aacgacgagc tgctgcccgt tgcgcgtgcg tttagtcagt tcctgaacct ggccaacacc
    13441 gccgagcaat accacagcat ttcgccgaaa ggcgaagctg ccagcaaccc ggaagtgatc
    13501 gcccgcaccc tgcgtaaact gaaaaaccag ccggaactga gcgaagacac catcaaaaaa
    13561 gcagtggaat cgctgtcgct ggaactggtc ctcacggctc accctaccga aattacccgt
    13621 cgtacactga tccacaaaat ggtggaagtg aacgcctgct taaaacagct cgataacaaa
    13681 gatatcgctg actacgaaca caaccagctg atgcgtcgcc tgcgccagtt aatcgcccag
    13741 tcatggcata ccgatgaaat ccgtaagctg cgtccaagcc cggtagatga agccaaatgg
    13801 ggctttgccg tagtggaaaa cagcctgtgg caaggcgtac caaattacct gcgcgaactg
    13861 aacgaacaac tggaagagaa cctcggctac aaactgcccg tcgaatttgt tccggtccgt
    13921 tttacttcgt ggatgggcgg cgaccgcgac ggcaacccga acgtcactgc cgatatcacc
    13981 cgccacgtcc tgctactcag ccgctggaaa gccaccgatt tgttcctgaa agatattcag
    14041 gtgctggttt ctgaactgtc gatggttgaa gcgccccctg aactgctggc gctggttggc
    14101 gaagaaggtg ccgcagaacc gtatcgctat ctgatgaaaa acctgcgttc tcgcctgatg
    14161 gcgacacagg catggctgga agcgcgcctg aaaggcgaag aactgccaaa accagaaggc
    14221 ctgctgacac aaaacgaaga actgtgggaa ccgctctacg cttgctacca gtcacttcag
    14281 gcgtgtggca tgggtattat cgccaacggc gatctgctcg acaccctgcg ccgcgtgaaa
    14341 tgtttcggcg taccgctggt ccgtattgat atccgtcagg agagcacccg ccataccgaa
    14401 gcgctgggcg agctgacccg ctacctcggt atcggcgact acaaaagctg gtcagaggcc
    14461 gacaaacagg cgttcctgat ccgcgaactg aactccaaac gtccgcttct gccgcgcaac
    14521 tggcaaccaa gcgccgaaac gcgcgaagtg ctcgatacct gccaggtgat tgccgaagcg
    14581 ccgcaaggct ccattgccgc ctacgtgatc tcgatggcga aaacgccgtc cgacgtactg
    14641 gctgtccacc tgctgctgaa agaagcgggt atcgggtttg cgatgccggt tgctccgctg
    14701 tttgaaaccc tcgatgacct gaacaacgcc aacgatgtca tgacccagct gctcaatatc
    14761 gactggtatc gcggcctgat tcagggcaaa cagatggtga tgattggcta ttccgactca
    14821 gcaaaagatg cgggcgtgat ggcagcgtcc tgggcgcaat atcaggcaca ggatgcatta
    14881 atcaaaacct gcgaaaaagc gggtattgag ctgacgttgt tccacggtcg cggcggttcc
    14941 attggtcgcg gcggtgcacc tgcccatgcg gcactgctgt cacaaccgcc aggaagcctg
    15001 aaaggcggcc tgcgcgtaac cgaacagggc gagatgatcc gctttaaata tggtctgcca
    15061 gaaatcaccg tcagcagcct gtcgctttat accggggcga ttctggaagc caacctgctg
    15121 ccaccgccgg agccgaaaga gagctggcgt cgcattatgg atgaactgtc agtcatctcc
    15181 tgcgatgtct accgcggcta cgtacgtgaa aacaaagatt ttgtgcctta cttgcgctcc
    15241 gctacgccgg aacaagaact gggcaaactg ccgttgggtt cacgtccggc gaaacgtcgc
    15301 ccaaccggcg gcgtcgagtc actacgcgcc attccgtgga tcttcgcctg gacgcaaaac
    15361 cgtctgatgc tccccgcctg gctgggtgca ggtacggcgc tgcaaaaagt ggtcgaagac
    15421 ggcaaacaga gcgagctgga ggctatgtgc cgcgattggc cattcttctc gacgcgtctc
    15481 ggcatgctgg agatggtctt cgccaaagca gacctgtggc tggcggaata ctatgaccaa
    15541 cgcctggtag acaaagcact gtggccgtta ggtaaagagt tacgcaatct gcaagaagaa
    15601 gacatcaaag tggtgctggc gattgccaac gattcccatc tgatggccga tctgccgtgg
    15661 attgcagagt ctattcagct acggaatatt tacaccgacc cgctgaacgt attgcaggcc
    15721 gagttgctgc accgctcccg ccaggcagaa aaagaaggcc aggaaccgga tcctcgcgtc
    15781 gaacaagcgt taatggtcac tattgccggg attgcggcag gtatgcgtaa taccggctaa
    15841 tcttcctctt ctgcaaaccc tcgtgctttt gcgcgagggt tttctgaaat acttctgttc
    15901 taacaccctc gttttcaata tatttctgtc tgcattttat tcaaattctg aatatacctt
    15961 cagatatcct taaggaattg tcgttacatt cggcgatatt tttttcaaga caggttctta
    16021 ctatgcattc cacagaagtc caggctaaac ccctttttag ctggaaagcc ctgggttggg
    16081 cactgctcta cttttggttt ttctctactc tgctacaggc cattatttac atcagtggtt
    16141 atagtggcac caacggcatt cgcgactcgc tgttattcag ctcgctgtgg ttgatcccgg
    16201 tattcctctt tccgaagcgg attaaaatta ttgccgcagt aatcggcgtg gtgctatggg
    16261 cggcctctct ggcggcgcta tgctactacg tcatctacgg gcaggagttc tcgcagagcg
    16321 ttctgtttgt gatgttcgaa accaacacca acgaagccag cgagtattta agccagtatt
    16381 tcagcctgaa aattgtgctt atcgcgctgg cctatacggc ggtggcagtt ctgctgtgga
    16441 cacgcctgcg cccggtctat attccaaagc cgtggcgtta tgttgtctct tttgccctgc
    16501 tttatggctt gattctgcat ccgatcgcca tgaatacgtt tatcaaaaac aagccgtttg
    16561 agaaaacgtt ggataacctg gcctcgcgta tggagcctgc cgcaccgtgg caattcctga
    16621 ccggctatta tcagtatcgt cagcaactaa actcgctaac aaagttactg aatgaaaata
    16681 atgccttgcc gccactggct aatttcaaag atgaatcggg taacgaaccg cgcactttag
    16741 tgctggtgat tggcgagtcg acccagcgcg gacgcatgag tctgtacggt tatccgcgtg
    16801 aaaccacgcc ggagctggat gcgctgcata aaaccgatcc gaatctgacc gtgtttaata
    16861 acgtagttac gtctcgtccg tacaccattg aaatcctgca acaggcgctg acctttgcca
    16921 atgaaaagaa cccggatctg tatctgacgc agccgtcgct gatgaacatg atgaaacagg
    16981 cgggttataa aaccttctgg atcaccaacc agcagacgat gaccgcccgc aataccatgc
    17041 tgacggtatt ttcgcgccag accgacaagc agtactacat gaaccagcaa cgtacgcaga
    17101 gtgcgcgtga atacgacacc aacgtgctga agccgttcca ggatgtgctg aatgaccctg
    17161 cgccgaagaa actgatcatc gttcatctgc tgggtacgca tatcaaatac aaataccgct
    17221 acccggaaaa tcagggcaag tttgatggca ataccgatca tgttccgcca ggattaagcg
    17281 cagaagagct ggaatcatat aacgattatg acaacgctaa cttgtataac gatcatgtgg
    17341 ttgccagcct gattaaagac tttaaagcgg cagacccgaa cggattcctg gtttacttct
    17401 ctgaccacgg tgaagaggtt tacgacacgc cgccgcacaa aactcagggg cgtaacgaag
    17461 acaacccgac gcgccacatg tacaccattc cgttcctgct gtggacgtcg gaaaaatggc
    17521 aagcgactca tccccgtgat ttctcacagg atgtcgatcg taaatacagc ctggcggaac
    17581 tgatccacac ctggtcagat ttggcgggct tatcttacga cggttacgat ccaacccgtt
    17641 cagtggtgaa tccgcagttc aaagaaacta cccgctggat tggtaacccg tacaagaaaa
    17701 acgcgctgat cgattacgac actctgccgt atggcgacca ggtaggtaat cagtaattat
    17761 cagccaggcg ctatttgccg gatgcgacgc ttgacgcgtc ttatccggcc tacagatcgc
    17821 ggcggtaggc tggataaggc gtttacgcca catccggcaa tacaactgcc gcaatgttta
    17881 catcgaactc ccctcaggcc acacttttca ggtggccttt tttaataatg ccagttactg
    17941 ttttcccgtc tacataccta agggaataac atgtatcacg acatcagcta tctgctctcc
    18001 cgcctgatca acggcccgct gtccctgcgc cagatttact ttgccagcag taacggcccg
    18061 gtgcccgatc ttgcgtatca ggtcgatttt ccacggctgg aaattgtgct ggaaggtgag
    18121 tttgttgata ccggcgctgg agcaacgtta gttcccggcg atgtgctgta cgttgccgct
    18181 ggtggctgga atttcccaca atggaaaaca cccgctacca cctttagcgt gctgtttggc
    18241 aaacagcaac tcggcttcag cgtcgtgcaa tgggatggca aacaatatca aaatctggcg
    18301 aagcaacacg tcgcccgacg cggcccacgt ataggttctt ttctgctaca aacgctcaat
    18361 gaaatgcaaa tgcagccgca ggagcagcaa acggcaaggc ttatcgtagc cagcctgctt
    18421 agccactgcc gcgatttgct cggcagccaa atccagaccg cctcacgcag ccaggcacta
    18481 tttgaagcta ttcgcgatta tatcgacgaa cgctatgcct ccgcgcttac ccgcgaatct
    18541 gtcgcacagg cattctatat ttcaccaaac tatctctcgc acctgtttca aaaaacgggg
    18601 gccattggtt ttaacgaata cctgaatcac acgcgactgg agcacgctaa gacgttattg
    18661 aaaggctatg acctgaaggt aaaagaagtg gcgcacgcct gcggctttgt cgacagcaac
    18721 tacttctgcc gtctgtttcg taaaaacacc gaacgctcgc cgtcggaata tcgccgacag
    18781 taccacagcc aactgacaga aaaaccgact actccagaat aagatgggtt tgctgcggcg
    18841 cagaaagcac tttgcgcacc gcgctcatta cccgctgcgg ctcacgcagg aatgcgtaga
    18901 tgctgcattg cacatagcgg caatgttcaa atcgctcggc accggcaagc tcgatatccg
    18961 taatcaacag agcaacatcc gcccgacgga tatcctcgtc cgctaaacga ttctccgttc
    19021 ccagcgcgcc ctgagtttca atgctgacgc cccacttctc taactggcac aacttttcca
    19081 gccgttccgc cgccatataa gtatgcgcca cgccgcttac gcaggcggtt actgccacca
    19141 ggtatgccat tttaacctcc cacggtaacc tgaaatccgg cccgttctgc catttcgcgc
    19201 atcgttgcca catcggctga tgacggcgca gcaacatcct tcatcgacca tgttttcccc
    19261 agcaggcggt atttcggctc gccgtactga tgaaacggca acagatggat ctgcctgata
    19321 ttcagcggga tcagcacgtc cagcgcctgc tgcatattct cccggctgag tgtgaaaccg
    19381 gggatcagcg gcaaacgcgg gatcacgttg acaccctcac tcaccagcaa acgcaggttc
    19441 tccagtacgc gtggcaggtt catcttcacc acatcccgcg cctgagtcgc gtccataatt
    19501 tttaaatcga acaacacttc atcgcacaat ttcgccagcg gtaacagctt gctggctggt
    19561 gcgtctccgg cagtttcaat ggcacatgag acaccccaca gccgcagtcg ctgtaaaaaa
    19621 cgggtagcaa actccgcctg cattaacact tcgccgccag aaagcgtgac gccgccgccg
    19681 gacgtgcgaa aaaaaatgtc atctttcatc acttcccgtt ccagagcgtc aaggctgata
    19741 tcgcgaccaa tccgttcaaa cgccccggag gggcattcat ccgcatcacg caaacatttc
    19801 gcacagtgca gacatttcgc ctctctgcgt accgtctgga ttttgccgga gatcgactcc
    19861 ggattagcac accacgggca aagatgcgga cagcctttaa aaaagaccac cgtacgaatg
    19921 ccctcgccgt cattcagtga ataacgctga atgttgaaaa tgcgcgccac atcattgagg
    19981 cgcgtttcca caacgttaca gctgatgcgc tgtccggcgg atgatgtcat cctggatctc
    20041 cttcgacagt tcgacaaaga aggcgctgta tccggcaacg cgcaccacca gcccggcata
    20101 atcttgtggg cgctgttgcg cttcccacaa cgtgtcggcg ttcaccacgt taaactgaat
    20161 atgctgcaac ttaagctggg taaacgcccg taagaagtcg gccagtttgc gtaatcccgc
    20221 ttcaccttcc agggtcgccg gagtgaattt cacgttcagc aacgtaccgt tagacagcag
    20281 cgtattatcg agcttactga ctgacttcag taccgctgtt ggcccttgtt cgtcctggcc
    20341 cagcataggt gacaagccgc cgtctgccag ctgttctccg gcaaaacgac cgtctggcgt
    20401 cgcgccaacc accgatccca acgggacgtg agcagaaacg gtatacgatc ccggcgtgaa
    20461 gtaaccaccg cgcgggttct ggtatttttc cacttctttg cagtagtggc gcaacagttc
    20521 ggcgctaatg ttatccacct cgtcgatatc gttaccatat ttctcaaagc ggttaattaa
    20581 gcgagcgcgg actttttcgc cttctggcgt tgcaaagttg gcttttaata ccgacagcaa
    20641 ttcgtcaaaa cttaaacgct gttgctcaaa aaccatccct ttcaacgcat ggagagaatc
    20701 gctcaggttg gcgataccga tcccctgtac gccggagaag ttataacgcg cgccgccatc
    20761 ggtaatatcg cgaccttttt ccagacaatc gctgataaaa gacgagagca gcggtacagg
    20821 tgcccagtcg cgatggccga tatcacaaat attgctgccc tcaaccatca gggtgatgta
    20881 gtggctgatc ttggcgcgga tctgctccag taaaccttca taagtcagcg cagcattgcc
    20941 ttcattttca tgcaggcaga tttccatcac tttcagcaga ttaaacatcg cgatgtcatg
    21001 caagccgtag gttctgccgg gaatagataa ttccacacag cccactacgg aatagtcgcg
    21061 cgcatcttcc agcgaaacgc cacggttgag gaacgctggt accaccactt catcgttaaa
    21121 gatttgcgga ataccggtgc cgaggcgaat ggtttcggcg gttttcatca ggaatggcgt
    21181 gtcgatcaag gcgttagtgc gcacgccgag gttcggttgc ggtaattgca cgctttgata
    21241 ggcgtcaagg caaaggaacg aaagcacgtt caccgcgcta cgtccgttct cggttaaccc
    21301 gccgagcagt gcggtatagc cggtcgggaa acctgcgaaa taacgcgcgc tgctggtgga
    21361 gcgcaacagc acgatgtcgt tgcatttcac ccataaagat tcgagcagtt ctttcaggaa
    21421 cgccggatct tcgcccgggg ttaaagatgc ctgatagaac ggcaacatat actggtcgaa
    21481 gcgccctaaa gacagcgaac tggcgttgga ttcgtattgc agaatgatgt tcatgtacca
    21541 gaataactgg cacgcctgcc agaacgtctg cggcttatgt tcggcgttat ggcgagagat
    21601 ctccgcaata gtcagcagtt cttcgcgacg ctgggcatct gtgcagtttg ccgccatcgt
    21661 ttccgccagt acagcgtaac gcaaaatgtg tttctgcgag gcttccagca gtaacagcgc
    21721 tgcctgataa aagtgattct ccggctgttg ctgacaatgt tgctgcatct gtgccaccaa
    21781 ctcccccagc ccgtgattca acaggcgtgg gtaatcaata ataatgtgcc cctggccttt
    21841 atccgtctgg ttaatgctga aaatctgcgt gctggtcgcg gcttttactt catccgtcat
    21901 ctgcccgttg atgaaatctt tcatcgaacg tttttcccag tacgggaaca actcttcacg
    21961 atagatacgt ttatcttctt cgctgatagc aaagcggtcc tgcggacgcg tcgggaattg
    22021 atccagctct ttcagcagcc agtaagggtc catttccggc gacataatcc cggcgcgcgg
    22081 ttttacggtg cggttaccgg caatcagttc ttcatcacga atcgaaattt caacatgttc
    22141 aaggatatac gctgttgctt tcgcccggcg caatatcacc ggttcgcctt cggtttgccg
    22201 atggctggcg gtataaagca gcgcccgctc cagcgagatt tcacgggtat tggcaaacag
    22261 tgcagttttg aggcgagaga tacgattcgt catgaggaac tcctgtggga aagcgaaccc
    22321 gcccttgcga gcgggagaag aacttaagcg gtttgcgcca ggtgagcttc aattttgctc
    22381 ataatggcgt cagcacgttt tactgcatcg ctgatgttga cgcgcacgat ggtcttgcca
    22441 gcaaaacgct cttcaaactt gatgccgata tctttggtca gaatcaccat atcagcgctc
    22501 gccacatctt ccgcagtcag ttcattttcc agaccaattg acccctgggt ttctactttc
    22561 acttcccagc ctttcgcttt cgcggcactt tccagcgcct ctgctgccat ataggtatgg
    22621 gcaacacctg aaggacatgc ggttactgca ataattttcg tcatggctcg ttcctcaatt
    22681 aattaatttc aaaatccaaa tccaggtcgt cttctttttc atcagtcgaa gacccatttt
    22741 tacgcgccag acttttcagc acgttcacac aaacagccgt caccactgct ccgacagcca
    22801 ctgctgcgat ataacccagc ttgccttcaa ccaccggcag cacaatcaga ccaccccagc
    22861 ctgcatagca ctgcgcaccg accagcgccg cagttactgc accacaaact gaaccgacca
    22921 tgatggaagg aatgacacgc agcggatcgg cagcggcgaa aggaatcgcc ccttcagtaa
    22981 cgccaacaca gcccatcacc agtgccgctt tgccggtttc gcgctcttct gcggagaaat
    23041 ttttacgacc aatcagcgtc gccagcccca tccccagcgg cgggatacaa atacccacgg
    23101 cagcgatgac caccacggta taaacaccct gagcaacgca aatcagcatg aaggcatagg
    23161 ctactttgtt aaccggaccg cccatatcga acgccagcat cagacccatg atcaccgcca
    23221 gcataacaat gctgccctgc tgcatcccct gaagccactg agtcaggctg ttggtcaacg
    23281 cccctacagg ctcgcccaag ccccacatca tgatgcctgc ggtaatcagt gtgccgacga
    23341 taggaataat gaagataggc atcaccgagc gcagaacttt atgcaccgga attttcttca
    23401 ggtaatgcac cacgatgccg ccgataatcc cggcgatcag tgcaccaaag aagcccgcac
    23461 caaagctgtt accaacccag gcaccgatag cgcacggagc cagcgcagaa cgctctgcaa
    23521 tggagtaacc aatgtaagcg gcgaggaaag gcaccatcag cgtcaaacct gcaacgccga
    23581 tatcaaacag ttttttcaaa ttcggatcgg ctacggcatc cggcactgcg cctttgccat
    23641 acaacatgac ggaaaccgcc agcaaaatac cgcccgatac cacgaaggga atcatgtgtg
    23701 aaacgcccgt cattaaatgc tgacgggtat ttttcaggat ctgcaccaac tcattcataa
    23761 gctgctcccg ggcttgcgaa agcccatgtc catatttttg atggaggaaa caataggcaa
    23821 tcgcaggcgg agcaaacagt ggataaaaag gccatttact ggacttttgt aatatcagta
    23881 caaaaatgcg atccgcctca taacttgcga taaaccggaa gcatgccgca aaacggcgtc
    23941 ttgtgagacg cgtcgcaaat tgacggttac attacatttg tccagctttt ggcagatttg
    24001 tcacatggca ggccgttgcc gtgcgctctt atcctgtaaa cagaaaaccg taccgggaga
    24061 gaggcaatgg ccctgattgt ggaatttatt tgtgagctac ctaacggcgt acatgcgcgt
    24121 ccggcaagcc acgttgaaac gctgtgtaat actttttcat cacaaattga gtggcataac
    24181 ctgcgcactg accgcaaggg caacgccaaa agcgcccttg cgctgattgg caccgatacg
    24241 ctggcgggcg ataactgcca gttactgatt tccggggccg acgaacagga agcgcaccag
    24301 cgtttaagcc aatggctgcg cgatgaattc ccccactgcg atgcgccgct ggcggaagtt
    24361 aaatctgacg aactggaacc actgccggtt tcactgacca atctgaatcc gcaaattatc
    24421 cgcgcccgca ccgtgtgcag cggtagtgca ggcggcattc tgacgccgat ctcttcttta
    24481 gatctcaatg cgctgagtaa tcttcccgca gccaaagacg ttgacgccga gcaatccgca
    24541 ctggaaaatg gcctgacgct ggtactgaaa aacattgagt ttcgtctgct ggatagcgac
    24601 ggtgctacca gctcgatact ggaagctcac cgatccctgg ctggcgatac atctttacgt
    24661 cagcacttac tgacaggcgt cagcgaggga ttaagctgtg ccgaagcgat tgtcgccagt
    24721 gcgaatcact tttgcgaaga gttcgcccgt tccagcagca gctacctgca agaacgtgcc
    24781 ctggacgtac gcgacgtctg cttccagtta ctccagcaaa tctacggtga gcaacgcttc
    24841 ccggcaccgg gcaaactgac gcagcccgcc atttgtatgg ctgatgaact gactcccagc
    24901 cagttcctcg aactggataa aaatcacctc aaaggtctgt tactgaaaag tggcggcacc
    24961 acctcacata ccgtaattct tgcccgttcg ttcaacattc cgacactggt tggtgtagat
    25021 attgatgccc ttactccgtg gcagcatcaa acgatttata tcgacggcaa cgccggggcg
    25081 attgtggttg agccagggga agccgtggcc cgttattatc agcaagaagc ccgcgtacag
    25141 gacgcccttc gtgagcaaca gcgtgtctgg ctgacccaac aagcccgtac cgctgacggt
    25201 atccgcattg aaattgccgc taacatcgct cactccgtgg aagcgcaggc cgcattcggc
    25261 aatggtgcgg aaggcgttgg tttgttccgc actgaaatgc tctatatgga tcgcaccagt
    25321 gcaccgggcg aaagcgagct gtacaacatt ttttgtcagg cgctggagtc tgccaacgga
    25381 cgcagcatta ttgtgcgcac tatggacatt ggcggcgaca aacccgttga ttatctgaac
    25441 attccggcag aagctaaccc gttcctcggt tatcgcgctg tgcgtattta tgaagaatac
    25501 gcctcgctgt tcaccacaca actacggtcg atcctgcgcg cctccgctca cggcagcctg
    25561 aaaatcatga tcccgatgat ctcctcaatg gaagagatct tatgggtgaa agaaaaactg
    25621 gcggaagcca aacagcaact gcgtaacgaa cacattccgt ttgatgagaa gatccagctt
    25681 ggcattatgc tggaagtacc gtcggtgatg ttcatcatcg atcaatgctg cgaagagatt
    25741 gatttcttta gtattggtag taatgacctg acgcaatatc tgctggcagt ggatcgcgat
    25801 aacgctaagg ttactcgtca ctacaacagc ctgaatccgg cattcttgcg ggcgctcgat
    25861 tacgccgtgc aggcggtgca tcgccagggc aaatggattg gtctgtgcgg tgagctggga
    25921 gcgaaaggtt ccgtgctgcc gttgctggtc ggcttagggc tggatgaact cagcatgagc
    25981 gcaccatcaa ttccggcggc gaaagcgcgg atgacgcaac ttgatagccg tgagtgccgc
    26041 aagttgctca accaggcaat ggcctgccgt acttcgctgg aagtggaaca cctgctggcg
    26101 caattccgca tgacccaaca ggacgcaccg ctggtcaccg ccgagtgcat cacgctggaa
    26161 agtgactggc gcagcaaaga agaagtgctc aaaggcatga ccgataacct gctgctggcg
    26221 ggccgctgcc gctatccgcg taaactggaa gccgacttat gggcgcgcga ggccgttttc
    26281 tctaccggtc tgggctttag ttttgccatt ccacacagca aatcagaaca cattgagcaa
    26341 tccaccatca gcgtggcgcg tctgcaagcg ccggtgcgct ggggcgatga tgaagcgcaa
    26401 ttcatcatta tgttaaccct gaacaaacac gctgcgggcg atcagcacat gcgcattttc
    26461 tctcgcctcg ctcgccgcat catgcacgaa gaattccgta acgcgctggt taacgccgcc
    26521 tctgccgacg ctatcgccag cctgctgcaa catgaactgg aactgtaaaa ggaaacatca
    26581 tggaactgta tctggacacc gctaacgtcg cagaagtcga acgtctggca cgcatattcc
    26641 ccattgccgg ggtgacaact aacccgagca ttatcgctgc cagcaaggag tccatctggg
    26701 aagttctgcc gcgtctgcaa aaagcgattg gtgatgaggg cattctgttt gctcagacta
    26761 tgagtcgtga cgcgcagggt atggtggaag aagcgaagcg cctgcgcgac gccattccgg
    26821 gcattgtggt gaaaatcccg gtgacttccg aaggtctggc cgccattaag atgctgaaaa
    26881 aagagggtat tactacgctt ggcaccgcag tgtacagtgc tgcacaaggg ttactggccg
    26941 cgctggcagg ggcaaaatac gttgctccgt atgttaaccg cgtagatgcc cagggcgggg
    27001 acggcattcg tacggttcag gagctgcaag cgctgttaga aatgcacgca ccagaaagca
    27061 tggtgctggc ggccagcttt aaaacgccgc gtcaggcgct ggactgttta ctggcaggat
    27121 gtgaatccat caccctgccc ttagatgtag cgcaacaaat gctcaacacc cctgcggtag
    27181 agtcagctat agagaagttc gaacacgact ggaatgccgc atttggcact actcatctct
    27241 aaaggagcaa ttatggaccg cattattcaa tcaccgggta aatacatcca gggcgctgat
    27301 gtgattaatc gtctgggcga atacctgaag ccgctggcag aacgctggtt agtggtgggt
    27361 gacaaatttg ttttaggttt tgctcaatcc actgtcgaga aaagctttaa agatgctgga
    27421 ctggtagtag aaattgcgcc gtttggcggt gaatgttcgc aaaatgagat cgatcgtctg
    27481 cgtggcatcg cggagactgc gcagtgtggc gcaattctcg gtatcggtgg cggaaaaacc
    27541 ctcgatactg ccaaagcact ggcacatttc atgggtgttc cggtagcgat cgcaccgact
    27601 atcgcctcta ccgatgcacc gtgcagcgca ttgtctgtta tctacaccga tgagggtgag
    27661 tttgaccgct atctactgtt gccaaataac ccgaatatgg tcattgtcga caccaaaatc
    27721 gtcgctggcg ctcctgcacg tctgttagcg gcgggtatcg gcgatgcgct ggcaacctgg
    27781 tttgaagcac gcgcctgttc ccgtagcggc gctaccacta tggcgggcgg caagtgcacc
    27841 caggcggcgc tggcactggc agaactgtgc tacaacactc tgctggaaga aggcgaaaaa
    27901 gcgatgcttg ccgccgaaca gcatgtagta acccctgcgc tggaacgcgt gattgaagcg
    27961 aacacctatt taagcggtgt tggttttgaa agcggcggac tggcggcggc tcatgcggtg
    28021 cataacggcc tgaccgctat cccggacgcg catcactatt atcacggtga aaaagtggca
    28081 ttcggtacgc tgacgcagct ggtactggaa aacgcgccgg ttgaggaaat cgaaaccgta
    28141 gctgcattta gccatgcggt aggtttgcca ataactctcg ctcaactgga tattaaagaa
    28201 gatgtcccgg cgaaaatgcg aattgtggca gaagcggcat gtgcagaagg tgaaaccatc
    28261 cacaacatgc ctggcggcgc gacgccagat caggtttacg ccactctgct ggtagccgac
    28321 cagtacggtc agcgtttcct gcaagagtgg gaataaccta ctccaaactc ccggcttgtc
    28381 cgggagtttg aacgcaaaat tgcctgatgc gctacgctta tcaggcctac gcaatctctg
    28441 caatatattg aatttgcgtg ctttttaggc cagataaggc gttcacgccg catccggcac
    28501 tctgtgccag taatctgaaa ctcctacgtt gttcaggagt tttttatccg cttctggaca
    28561 ttttctctac agaacaatcg ttagcccgga aggtcgaatc tgtgactctt attttcacgg
    28621 acgaaccaga tgaaagcttc actggcgctg ctcagcctgc taacggcatt taccagccac
    28681 tccttaaaat ctcccgccgt tccaccaacc gtggtacaaa tccaggccaa taccaacctt
    28741 gccatcgcag acggtgccag acagcaaatc ggcaacacgc tattttacga tccggcgtat
    28801 gtgcggctta cctatcctgg cggtgatgtc ccgcaagaac gcggtgtatg ttccgatgtg
    28861 gtgatccgtg cattgcgcag ccagaaggtc gatttgcaga aactggttca cgaagatatg
    28921 gcgaagaatt ttgccgagta cccgcaaaaa tggaagttaa agcgcccgga cagcaacatc
    28981 gatcaccgtc gggtgcctaa tctggaaacc tggtttagcc gccacaacaa aacacgcccc
    29041 atcagtaaaa atgccggtga ctatcaagcg ggcgatattg tctcctggcg attagataac
    29101 gggctggcgc atattggggt ggtgtcagat ggcttcgccc gcgacggtac gccactggtg
    29161 atacataaca ttggcgcagg cgcgcaggaa gaagatgtgc tgtttagctg gcggatggtg
    29221 gggcattatc gttattttgt taaataagcg atcgccccac atgaagaaaa aattcagatg
    29281 ctttttacac tgacagcttc ttttttctta cggctcacca gcgccagtgc cagcacaatc
    29341 agcacaatac cgctaccttc aacggccccc ggattctcac cgagcagcca ccaggaaaag
    29401 agtacgccgc aaacgggaac ggccagcgtg cttaagctgg caatactggc aggcaagttt
    29461 ttcaatacaa acaaccataa gctccacgcc agcgccgtcg ccagaatcgc actgtaggcc
    29521 agcgcccaga acacggtggg ctgccagtca atttcacgtt gcggcaccag taaagcgacc
    29581 acactcatca ccagcgccgc atagagcatc tgccaggatg ttaacgataa taaatccacg
    29641 cgcggatgac gggtgtatag gcgtttagca acaatcgcgc tcgcccccca actaacaccg
    29701 gagaggattg ccagcatggc acttttcatc gaagagaaat cgagttgcca cggctgcaac
    29761 accaaaaata agccgaaagc ggcaatcaga atcgcgaaat attgcccacg tcgcaggcgt
    29821 tcaccgagaa acaacgcggc gaaaatcacc acccagaacg gcatggtata gctcaggatc
    29881 gccaccttcc ccgcacctcc gctgaccaac gcccactgcg ccagaccaac catcccgcag
    29941 gtttgtaaca gggcaatggc taaggtgtat ttaaacggcg tcgggtgcat tccgcgacca
    30001 cgtaataaaa ggacgatgaa taaaacgaga gcgccgaaaa tgcagcgtaa ggcggtaaag
    30061 tcgaaggcac cgatgtaact ggtgacttgc ttcatgaaaa tccagctata actccagata
    30121 agtgtgagca caaccaggcc actgattgcc agtgggttgc tctttcctgc ggcagacatg
    30181 agagatccag tgtgtagttt tcctgtggac actatacgcc ggtaaagaaa cttcagcgac
    30241 tgcctgaaag acagtcactg aagaaggtca gattacagca ggtcgaaacg gtcgaggttc
    30301 atcactttca cccatgccgc tacgaagtct ttaacaaact tctcgtgggc atcgctgctg
    30361 gcgtaaactt ccgctaccgc acgcaggaca gagttagaac caaataccag atccgcacgg
    30421 ctggctgtgt atttcacttc gccggtttcg cggtcacgtc cttcgaacag ctctttcgat
    30481 tcgtcagtcg ctttccactc gtaacgcata tccagcaagt tcacgaagaa gtcattgctc
    30541 aatacgccaa cgcggtcagt gaagacgccg tttttgctgc tatcgaagtt ggcacccagt
    30601 acacgcatcc cgcccaccag cgcagtcatt tccggcgcgg tcagcgtcag ttgctgtgct
    30661 ttgtcgatca gcagtgactc ggtggtggaa acgtccagac gagcgcgata gttacggaaa
    30721 ccgtcagcaa ttggctccag cagctcaaac atctcaatgt cagtctgatc ctgacgcgca
    30781 tcaacgcgac ccggcgcaaa cggtacatga atgctcaaac ctgcggcgct tgcggctttc
    30841 tcaacaccaa ccacaccagc cagcacgatg atatccgcca gcgaggcttt accagactct
    30901 ttctggattt tctccagaac aggcagagca cgaacggctg cggcgttcac atcccagtcg
    30961 cgctgcggca ttaatgccag acgcgcaccg ttggcaccac cgcgtttgtc gccaccacgg
    31021 aaggtagaag cagatgccca ggccaccgat accagctcac taacagacag accagaatcc
    31081 gcaatcgcga atttcagatc gataatgtcc tgctcggtcg ggttgtagat cggctgcggc
    31141 agcggatctt gccagatcag atcttctttc ggcacttccg gcccgatgta gcgagatttc
    31201 ggccccatat ccctgtgcgt cagtttgaac caggcacggg caaaggcttc gttgaacgcc
    31261 tgcggatcgt tgaggaaacg acgagagatc ttctcgaact caggatcaaa acgcagcgtc
    31321 aggtcggtca ccagcattgt cggtttacgt ttcttcgacg gatcgaacgg atccgggata
    31381 atttccggtg cgtctaccgc ttcgaactgg attgctccag ccggactgcg ggtctgtacc
    31441 cactcatatt tgaacaggtt ctcgaagaaa tagttgctcc actgggtcgg cgtctgggtc
    31501 cagacaactt ccagaccaga ggtaatggca tctgcgccaa cgccgctgcc gtaagtgctc
    31561 gcccagccta aaccttgttc ttcaatcggt gcagcttctg gatcaggacc tacatttgat
    31621 gtcggaccgg caccgtgagt tttacccagc gtatgaccac ccgcaatcag cgccacggtt
    31681 tcttcgtcgt tcatgcccat gttgccgaag gtcgcacgga tagctgctgc cgcagaaagc
    31741 ggttcgccgc tgtgatccgg gccttccggg ttaacgtaaa tcagacccat ctcggttgca
    31801 cccagcggtg ctttcgccag cgcttccgga tgacggtgag tcagccaggc tttttcatca
    31861 ccccagttaa catccagatc cggttcccag acgtcttcac gaccggcacc aaaaccgaag
    31921 gtacggaagc cggagttttc cagcgccacg ttacctgcga ggataaacag gtcggcccag
    31981 gagattttct gaccatattt ctgtttgatt ggccacaaca ggcgacgcgc tttatcaagg
    32041 cttacgttat ccggccagga gttcagcggt gcaaaacgtt gctgaccacg acccgcgccg
    32101 ccgcgtccat cgattgaacg gtaagtacct gcgccgtgcc aggccatacg aataaacaga
    32161 ccggcgtagc tgccccagtc ggctggccac cacggctgag attctgtcag cagggctttc
    32221 agatcttttt tcaggccgta gtaatctaat ttgctgaatt ctttgcggta gtcaaagtcc
    32281 tcacccagcg ggttagaacg attagaatgt tggtttaaca ggtcaacacg gagttgattt
    32341 ggccaccagt cgcgagtggt tgtgcccgcc cccgcgctct ggtcgtgacc gccctgatgg
    32401 aacgggcatt tgccagtggc tgtcgtgtta tggatatcgt ctgacgtgct catcaatgtg
    32461 ctcccctcta cagtgttacc gttacgatac acagcgttag agagaagtta taattgaatt
    32521 aatccacgga tgcgatagtt gagatcttac atattgttgg ttaaagagat gtaggtcaaa
    32581 ttgatcgtaa ttaagctttt ttgattatgt ccgaattcgg acaaatgctt tataaaaaag
    32641 gggtttagct tcatatcctt caagtaatga gggagaaaac aaggctgaca accttatttt
    32701 cccgccctca tttcgaggca gcattttgtg ctctgtttaa aatttgtgat cactgtgtga
    32761 ttttcacaaa agccacacta tttataaacc aggtcgaaca cccagcgtat ggcaaatcgc
    32821 gtaactcatt tcagcgcggt taagcgtata gaagtggaaa tctttcactc cttcacggct
    32881 taaaatcttc accatatcca tggcaatatt cgcgcccacc agtttgcggg tttcggcatc
    32941 atcatccagg ccgtcgaaca tttgcgccat ccacgccgga atacgcacgt tggtcatatc
    33001 ggcaaatttc ttcgcctgtt taaagttaga taccggcaaa attcccggaa taatttccac
    33061 atcaatgccc gccgatacac agcggtcacg aaaacgcagg tagctttcga catcgaagaa
    33121 gaactgagta atcgcgcggt tggctccggc atcgactttg cgtttcagat taagcaaatc
    33181 cgcctgagcg ctttttgctt ccgggtgaac ttccggatac gccgccacgg agatatcgaa
    33241 atctgccact tcttttaata gcgtcaccag gtcagaagca tacatttctg gcttaccact
    33301 tcccggcggc agatcgccac gcagcgccac gatatgacga ataccgttat tccagtagtc
    33361 gcgtgcaatg gtgcgcaact cgtcgggcgt cgcatcaatg caagtaagat gcggtgccgc
    33421 ttccagacca gtgcgatctt taatgccttt aataatgctg tgcgtacggt cgcgctcgcc
    33481 ggagttcgcg ccataggtca ccgatacaaa cttcggtttc aggctgctaa ggcgatcgat
    33541 ggagttccac agggtctgct ccatttcact ggtacgcggc gggaaaaact cgaacgaaac
    33601 gttaatctgc ccctggactt ctgccaggcg ctgattcagg gcatcccgct ggctggcgtg
    33661 aaaaaagctc ataccttacc tcatcaatcg cttgtagtta tatgttgtgt tgtgcacatc
    33721 tatccgttta gacgtccaga tgtaaagatg aaggaaaagc cgaagggcgt caaccgaaaa
    33781 atgaccaatg atggtgagga aaaatcaggg aagatgaaaa aacttcagga tggtaagaaa
    33841 aagaaaatcc ggtatgcaaa gcaaaccgga ctgcaaatat aaactaacca tgtacttatt
    33901 gcttcttgcc ggattcgtcg taaacgctta atccggccta atcctatgat aattcaatat
    33961 atttcattag aaaccataaa cccgaaaaca tgagtaccgg gcattattaa atttctgaaa
    34021 ttacaacaac tgtgccagcc ggttgatatc cgactgaatc gccccggcgg tgacgtcgcg
    34081 ccccgcgcca ggcccacgga tcaccagcgg gttgtcgcga taccagcggc tttcgatggc
    34141 aaagacgtta tcgcacggca gcagtgatgc cagcggatgg tcttcacgca ccgcctccac
    34201 gcccacacgc gctttgccgt tggcatcgaa acgcgccacg tagcgcagca ccagccccat
    34261 ttcgcgggcc gcttccagcc gttgcaccat ctgctcgtta agttcatcgc cattttcaaa
    34321 gaaatggtcg atgctgccgc cttcgcaatg agcaggcacc agcgattcca cacgtacctg
    34381 gtccggttca atgttgtaac ccgcttcacg cgccagaatt accagcttgc gcattacgtc
    34441 tttgccggag aggtcgtcac gcggatcagg ctcggttaag ccctgctgcc acgcctgatc
    34501 caccagctcg gtaaacggca cgctaccgtc gaattgcagg aacagccagg agagcgtgcc
    34561 ggagaagatc ccgctgatcg acaaaatagt atcgccgctg tcgatcagat cgcgcacggt
    34621 gtggttgatc ggcaagcccg caccgacggt ggcattgtac agccagtgac gcccggtttt
    34681 ttcgaaggcg tcgtggatct ggcgatattt attgctgtcg ctggctcccg ccagtttgtt
    34741 ggcgctgata acgtggaaac cgtggctggc gaaatcaaga tactgatcag caagctgctg
    34801 gctggcggta acgtccagca ccactaaatc atcatacgga tgggcgcgca tccacaggaa
    34861 caacgactct tcatcctgct caaccgcttc atcgttgaag aaggctaacg cgcggctggc
    34921 gtccagcccg tcatagctca acaggctacg gcggctgtcc accacacctg ccagcacaaa
    34981 ctcaaagccg gtacgtgccg aaagcgtgct ctgctcacgg gcgaacagtt ccagccaacg
    35041 ggaaccgata ttgcccttac cgaacaatac caggccgatg cgtttttctg cgcggaagac
    35101 ggactgatgc agcccctgaa tcaggctttc ggtcgggccg gtgcgcagta ctgccaccag
    35161 gctgatgccg tcatcggact gccaggtaaa ttcgaccggc tggcctttca gttgctgcca
    35221 gaagcggtgg caatgcagcg ggttacgggt gacgcctgca ccgaccatcg ccaccagcgc
    35281 cagcccctga cgcaggcgca gttcgccagg taatcccgct tcgtcgagga ttttcagcgc
    35341 actgtcggcc acttctgagg tgtagcaaaa ttgcagcaac tggcgatcgt tatgtacgcc
    35401 aaccgccagc gggcgtacct gcgcgcgttt caggatttgg tcgatctctt tatgcgccag
    35461 tttgaaatcc tgactggcgg gcacctgaaa ctcaatcaaa cagacatcat cgtggctggt
    35521 gacaatacgc gcaccagtac cggaggccag cacgcgttca atgcgcgtgg aaccttgatc
    35581 cggcgtgtag ctacagcgca gttgcaggtc gatttcgctg ccagaaaccg gctgtaaagt
    35641 acgggcgtga agaacgggag ccgccaggcg cgccagttcg ctggcctcat ccagacgcag
    35701 caacggcagc aggcaggcat ctttcacttt acgcgggtcg gcactgtata ccccggcgac
    35761 gtcgctccag atggttacgc gagaaacacc cgccagcgca ccgatttgtg tcgcggaata
    35821 gtcggaaccg ttacgcccca gcagcaccgt ttcaccggcg ttgttgcggc tgataaatcc
    35881 ggtcaccacc agacgtttgc ccggatgttg caccagcagc tgttgcagca acgggtaaga
    35941 aagcccttca tcaacctgcg gttgtgcggc gcgttcagcg cgtaaaaact cgcgggcatc
    36001 aagccaggcc gctggcagcc cttgttgatt aagtaccgca gacatcaggc gtgccgacca
    36061 tacttccccg tggcccacca cttccgcata cactgcgtcg ttaataccgc tgtcgagcag
    36121 cgccgccagg cgctcaaggt cgctgacaaa agcgctaatg aggctatcag cttcttcagc
    36181 gggtagcaga ccgctaatca gatcgcactg atagcgacgc agcgtttgtt gaacctgatg
    36241 cgcagagaga cgatcggtct ggcttagttt caaccagtta atcaactggt tagtggtgct
    36301 accggcggcg gaaaccacca tcatatcgtc aggctgagag tactccgcca taatgcccgc
    36361 aacacgcaaa taacacttca catcagccag actactgcca ccaaatttat gcagttgacg
    36421 acctttcgcc cctgcctgcg caatcacact catttttacc ccttgtttgc agcccggaag
    36481 ccattttcca ggtcggcaat taaatcttcg ccatcttcaa taccggtgga gatacgcagc
    36541 agcgtctcgg agatcccggc ggcagcacgc gcttctggtg ccatgcctgc atgtgtcatg
    36601 gttgcggcgt gagagattaa actttccact ccccctaatg attccgccag cgtaaacaac
    36661 gacagcccgc ccaggaaacg acgcagcgtc tgctcatcgc catccagttc aaaactcaac
    36721 attgcgccaa agcctttttg ctggcgcgcg gcaatttcat gcccctgatt ttccggcagc
    36781 gacgggtgat acagtttttt caccaacggc tgggtttgca gatatttcac aatcgcctgc
    36841 gcgttgcgct gggccagctc catacgcggc accagcgttc gcaacccgcg tagcagcaga
    36901 tagctgtcaa acgcgccgcc cgtcacgcca atattgtttg tccaccaggc aagttcagtg
    36961 acaacgtccg ggtctttagc aatcaccacg ccagccacca cgtcagagtg accgttcaga
    37021 tatttcgtac atgaatgcaa caccagatcg gcacccaatg ccagcggatt ttgtaatgcc
    37081 gggcttaaga aggtgttatc caccacgctc accgccccga cttcccttgc cagatggcag
    37141 attttcgcaa tatccacgac gcgtaacaat ggattacttg ggctttctac cagtaccagt
    37201 ttaggttttt ctgccagcgc tgcccgtaat gcctgttcat cgccttgatc aacaaacaac
    37261 acgcgatagc aaccgcgttt cgccagactg tcgaacaggc gatagctacc gccgtagcag
    37321 tcgtgcggcg caaccagcag atcgccaggt ttcaaaaaga cggtcgttac caagtgaatc
    37381 gcggacatgc cggtattagt aagtactgcg ccagcaccgc cttccagttc tgccagcgca
    37441 cgctgaacca catcgcgcgt tgggttgccg cgacgtgagt aatcatgcgc gcgcggttca
    37501 ttaaatccgg taaagttata ggtgctggaa agatggatgg gagggacaac gcaaccatac
    37561 tgttcgtcgt catttaaccc gctacgcact gcgatggtgg cctgtttacg cgtcatgtga
    37621 tgaagtttcc tgggctttgt cggtgaaatg tcaggcacca gagtaaacat tgtgttaatg
    37681 gacgtcaata catctggaca tctaaacttc tttacgtata gattgagcaa atcccaaata
    37741 gccgttaaaa ttatatgcat tatcacgccg acaggtgcat tacacgatgt cacggtaacg
    37801 cctgtacggt aaactatgcg ggtttacggt cagtacccac ataaattgtg tggtctggtc
    37861 tcaatttatt gacgaagagg attaagtatc tcatggctga atggagcggc gaatatatca
    37921 gcccatacgc tgagcacggc aagaagagtg aacaagtcaa aaagattacg gtttccattc
    37981 ctcttaaggt gttaaaaatc ctcaccgatg aacgcacgcg tcgtcaggtg aacaacctgc
    38041 gtcacgctac caacagcgag ctgctgtgcg aagcgtttct gcatgccttt accgggcaac
    38101 ctttgccgga tgatgccgat ctgcgtaaag agcgcagcga cgaaatcccg gaagcggcaa
    38161 aagagatcat gcgtgagatg gggattaacc cggagacgtg ggaatactaa agcaaaaaag
    38221 agcggcgcgg agtggaatcg cctgatgcgc tacgcttatc aggcctacgt catattgcaa
    38281 tttattgaat ttgcacgaac ttgtaggccg gataaggcgt tcacgccgca tccggcataa
    38341 acaacgagca cgttgtctgc gacccaccgc tttttataca tggacgttta actatgaaaa
    38401 acaggctgct gatcctcagc ctgctggttt ctgtacctgc ctttgcctgg cagccacaaa
    38461 ccggcgacat catctttcag atctctcgct catcgcaaag taaagcgatc caactggcga
    38521 cccattccga ttatagccac accggtatgc tggtgatacg caacaaaaag ccctacgttt
    38581 ttgaagcggt cggcccggtg aaatacaccc cgctcaagca gtggatcgcc catggtgaaa
    38641 agggcaaata cgttgttcgc cgcgttgaag gcggactgag tgttgaacaa cagcaaaaac
    38701 tggcgcaaac ggtaaaacgt tatctcggta aaccgtatga tttcagcttc tcatggagcg
    38761 acgatcgcca gtactgttcg gaagtggtgt ggaaggttta ccagaacgcg ctgggaatgc
    38821 gcgtgggcga gcagcagaag ttgaaagagt tcgatctcag caacccgctg gtccaggcta
    38881 agctcaaaga acgctacggc aaaaatattc cgcttgagga aacggtagtt tcaccacaag
    38941 ccgttttcga cgcgccacaa ctcaccacgg ttgccaaaga gtggccgctg ttttcgtggt
    39001 aatggatgat gaaaaaaata attcccgccg tagtctgttt gttgctttgt agttgcacga
    39061 cctttccgca aactgtcgct ccggtaaacg gtgatgcctc caattagttg aattaaggat
    39121 ataatactcg cttttaaggt tgtttcatgg tcacagttaa ccttcattgc ccccgttgcc
    39181 agtccgttca ggtttaccgt cacgggcaga acccaaaaag acatgatcga ttccgcggcc
    39241 gtgactgtca ccgggtattt cagctcagtt actgttacga gccccgtaaa ccgggagtta
    39301 aagaccaaat cgctgaaatg actttcaacg gtgccggggt tcgcgatact gcccgtacgc
    39361 tgaaaatcgg cattaataca gtcatccgaa ctttaaaaaa ctctcgccac gaagaataac
    39421 gtcatccccg gtcgctcatg ctgatgtggc actcatctgc gaacttagtc agcaatggag
    39481 ctttgtcggc agtaaagcgc gacagcactg gctctggtac gtgtacaaca cgaaaactgg
    39541 tggtgttctg gcctacactt ttggcccccg gactgacgaa acctgtcgtg aattgctggc
    39601 gctgcttacc cttttaccat cggcatgttg accactgacg aatggggtag ttacaccaga
    39661 gagttaccga aggaaaagca tctgactggt aagattttta ctcagcgcat tgagcgcaac
    39721 aatctgaccc tgagaacccg aatcaaacgt ctggcccgca gaaccatctg tttctcgcgc
    39781 tctgtagaac ttcatgaaaa agtcatcggt gcttttatcg aaaatatatg ttctactaat
    39841 tggagtcatc acccttctga tgagctttcc ttggatgatt accagactga atacacagcc
    39901 aaagattttt acctcattct gctctttttt ctcaaatgac atatattata ttttacatca
    39961 tattcatagg catatgacat tcccccgccc ccattatgtc attgtgggtt gatgttgggc
    40021 tttcataaaa aaaacatctt tttttgaact agcattaatt atatttactt tatatattaa
    40081 aatattttct ccatctggaa atattttagc cacacaatat aaagcagctg aatccgaatt
    40141 acttaaaaat atattatcat tacccataac tccagccatt aaattactgg agactaaagc
    40201 aaggaaataa caacattgta ttgccttcat tttaattact cccttgtctg ataattgtaa
    40261 ctccattccc tttagctttg ggattatatt ttagataggt gttatttggt gttcccaagt
    40321 aactacttgt attctttttt tcccattcca ttcatataag aattcgttaa atctttgctt
    40381 gaaaaattta gactatcata aacatcattt tcttttgtta ctttatttcc tttatcgtca
    40441 gagtaaaaac cgtgagtgtg ataatcgcct actctctctg aaccttcagg gcattttata
    40501 tttcgcaagt ctacactatc attatcattt gaacttatcg gattcatggg gaaatattca
    40561 ccttgcttct tacatattag tccaccatat tctttattct cgttaattga ttctccatta
    40621 cacat
//
LOCUS       DAJTMC010000013        40428 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.013, whole genome shotgun sequence.
ACCESSION   DAJTMC010000013 DAJTMC010000000
VERSION     DAJTMC010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40428
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.013"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(115..2436)
                     /locus_tag="OO130_000630"
     CDS             complement(115..2436)
                     /locus_tag="OO130_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HCR5364149.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFITRYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNARRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            complement(2533..3009)
                     /gene="greB"
                     /locus_tag="OO130_000631"
     CDS             complement(2533..3009)
                     /gene="greB"
                     /locus_tag="OO130_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HCR5364150.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCMENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            3237..3956
                     /gene="ompR"
                     /locus_tag="OO130_000632"
     CDS             3237..3956
                     /gene="ompR"
                     /locus_tag="OO130_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HCR5364151.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            3953..5305
                     /gene="envZ"
                     /locus_tag="OO130_000633"
     CDS             3953..5305
                     /gene="envZ"
                     /locus_tag="OO130_000633"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HCR5364152.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(5381..7003)
                     /gene="pckA"
                     /locus_tag="OO130_000634"
     CDS             complement(5381..7003)
                     /gene="pckA"
                     /locus_tag="OO130_000634"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HCR5364153.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            7382..9106
                     /locus_tag="OO130_000635"
     CDS             7382..9106
                     /locus_tag="OO130_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HCR5364154.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATTGMSGWLKWQTDGMNPWRAEKAIWD
                     FGCYLLLMAMLLLPWIQQSLRIRNCSSRYSYFYQSVWHNVLILLVIFLANGLTWLVLL
                     LWGELFKLVGIKFFNTLFFATDWFMYLTLGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVAAWALWLRVAQYGWTVDRLQGALAVLVLLVWSLGY
                     FVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQEQVSEKV
                     LAENVLIAPGSVKPDVTFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQAERILFAF
                     NDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTRPKAWRDLTVDGE
                     TLEINLSK"
     gene            complement(9241..10119)
                     /gene="hslO"
                     /locus_tag="OO130_000636"
     CDS             complement(9241..10119)
                     /gene="hslO"
                     /locus_tag="OO130_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HCR5364155.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSREHCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(10144..10545)
                     /gene="hslR"
                     /locus_tag="OO130_000637"
     CDS             complement(10144..10545)
                     /gene="hslR"
                     /locus_tag="OO130_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HCR5364156.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMAMAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            complement(10556..11224)
                     /gene="yrfG"
                     /locus_tag="OO130_000638"
     CDS             complement(10556..11224)
                     /gene="yrfG"
                     /locus_tag="OO130_000638"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HCR5364157.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            complement(11289..13424)
                     /gene="igaA"
                     /locus_tag="OO130_000639"
     CDS             complement(11289..13424)
                     /gene="igaA"
                     /locus_tag="OO130_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HCR5364158.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRSQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLDQQTDIDIYLDCHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            13744..14304
                     /gene="nudE"
                     /locus_tag="OO130_000640"
     CDS             13744..14304
                     /gene="nudE"
                     /locus_tag="OO130_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HCR5364159.1"
                     /translation="MSKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            complement(14469..17021)
                     /gene="mrcA"
                     /locus_tag="OO130_000641"
     CDS             complement(14469..17021)
                     /gene="mrcA"
                     /locus_tag="OO130_000641"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HCR5364160.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQGATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            17141..17920
                     /gene="hofM"
                     /locus_tag="OO130_000642"
     CDS             17141..17920
                     /gene="hofM"
                     /locus_tag="OO130_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HCR5364161.1"
                     /translation="MAFKIWQIGLHLQQQEAVAVAIVRGAKECFLQRWWRLPLENDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGMTSAKELAAALFVDPDS
                     VAICGEGGFDPWEAVSVRQPLLPPPGGDFAIALGLALGKAY"
     gene            17920..18459
                     /gene="hofN"
                     /locus_tag="OO130_000643"
     CDS             17920..18459
                     /gene="hofN"
                     /locus_tag="OO130_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HCR5364162.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTG
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLRKQRSQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            18443..18883
                     /gene="hofO"
                     /locus_tag="OO130_000644"
     CDS             18443..18883
                     /gene="hofO"
                     /locus_tag="OO130_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HCR5364163.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWATLYRLVDTTPFSEEKTLPFSPLDFQLPGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            18873..19277
                     /gene="hofP"
                     /locus_tag="OO130_000645"
     CDS             18873..19277
                     /gene="hofP"
                     /locus_tag="OO130_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HCR5364164.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDVLTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            19189..20427
                     /gene="hofQ"
                     /locus_tag="OO130_000646"
     CDS             19189..20427
                     /gene="hofQ"
                     /locus_tag="OO130_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HCR5364165.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNNNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGIEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEYERRELVVFITPRLVSSE"
     gene            20828..21349
                     /gene="aroK"
                     /locus_tag="OO130_000647"
     CDS             20828..21349
                     /gene="aroK"
                     /locus_tag="OO130_000647"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HCR5364166.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            21406..22494
                     /gene="aroB"
                     /locus_tag="OO130_000648"
     CDS             21406..22494
                     /gene="aroB"
                     /locus_tag="OO130_000648"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HCR5364167.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLKRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEIRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            22586..23872
                     /gene="damX"
                     /locus_tag="OO130_000649"
     CDS             22586..23872
                     /gene="damX"
                     /locus_tag="OO130_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HCR5364168.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTSSSDQTASGDKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPSTTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            23979..24815
                     /gene="dam"
                     /locus_tag="OO130_000650"
     CDS             23979..24815
                     /gene="dam"
                     /locus_tag="OO130_000650"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCR5364169.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            24833..25510
                     /gene="rpe"
                     /locus_tag="OO130_000651"
     CDS             24833..25510
                     /gene="rpe"
                     /locus_tag="OO130_000651"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HCR5364170.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            25503..26261
                     /gene="gph"
                     /locus_tag="OO130_000652"
     CDS             25503..26261
                     /gene="gph"
                     /locus_tag="OO130_000652"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HCR5364171.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALAWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     SEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            26254..27258
                     /gene="trpS"
                     /locus_tag="OO130_000653"
     CDS             26254..27258
                     /gene="trpS"
                     /locus_tag="OO130_000653"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HCR5364172.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            27527..28432
                     /locus_tag="OO130_000654"
     CDS             27527..28432
                     /locus_tag="OO130_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709156.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5364173.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLSQNNLCIALE
                     LGPHTYVGEHQLICRKGESGNVKRVGLDSRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            28449..28811
                     /locus_tag="OO130_000655"
     CDS             28449..28811
                     /locus_tag="OO130_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR5364174.1"
                     /translation="METRLNLLCEAGVIDKDICKGMMQVVNVLETECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYLLANLYGLWMAANEEV"
     gene            28895..30058
                     /locus_tag="OO130_000656"
     CDS             28895..30058
                     /locus_tag="OO130_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HCR5364175.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQVLENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLSVAHQ
                     GHLVQIPCHQVADAVEQGTDVITVFTLDKAREVSAAAVKAGRVQFVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVVAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLIQAR
                     DQLAKSGIALEQLNAPSATSCTSLPLLAQYGVTHAEPGHALTGTIPANQQGDQSERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     SLAGEFPVSSAVVLCFRTQIFVTRSDVVLVSGIHRGEPKIVGRYDSLGNSLGA"
     gene            30058..31284
                     /locus_tag="OO130_000657"
     CDS             30058..31284
                     /locus_tag="OO130_000657"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCR5364176.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINALGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFCDVIDRVEQALVSAGWQVERRGDELQFLWVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVREQVQVGRVITFGGLLTDSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDKKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICTNIQETDLAGHAEDVARYAERLQVVDRNLARLVDAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPLLVYQQGLAATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            31281..32159
                     /locus_tag="OO130_000658"
     CDS             31281..32159
                     /locus_tag="OO130_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HCR5364177.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLGVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGLIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRETPSQFFQ"
     gene            32170..32523
                     /locus_tag="OO130_000659"
     CDS             32170..32523
                     /locus_tag="OO130_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HCR5364178.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGSFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            32535..33839
                     /locus_tag="OO130_000660"
     CDS             32535..33839
                     /locus_tag="OO130_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HCR5364179.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIRPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            33851..34936
                     /locus_tag="OO130_000661"
     CDS             33851..34936
                     /locus_tag="OO130_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCR5364180.1"
                     /translation="MKTFPLQSLTLIEAQQKQFALVDTICRHFPGAEFLTSGDLGLTP
                     GLNQPRITQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITVDFNDLSALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVINRIRA
                     TLYSGGSQIQGAQALEVLRCLVFAPVMHAVQAGVSERLLALLNGGEVPEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQSE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(35088..35819)
                     /gene="phoB"
                     /locus_tag="OO130_000662"
     CDS             complement(35088..35819)
                     /gene="phoB"
                     /locus_tag="OO130_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator PhoB"
                     /protein_id="HCR5364181.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERVSAAPFCKKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(35919..36704)
                     /gene="frlD"
                     /locus_tag="OO130_000663"
     CDS             complement(35919..36704)
                     /gene="frlD"
                     /locus_tag="OO130_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HCR5364182.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDETLRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            complement(36701..37531)
                     /gene="frlC"
                     /locus_tag="OO130_000664"
     CDS             complement(36701..37531)
                     /gene="frlC"
                     /locus_tag="OO130_000664"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HCR5364183.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAMDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELYEYAENIGMDLILEPLTPYESNIVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIERGYEGYCTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            complement(37581..38603)
                     /gene="frlB"
                     /locus_tag="OO130_000665"
     CDS             complement(37581..38603)
                     /gene="frlB"
                     /locus_tag="OO130_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HCR5364184.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQAAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            complement(38624..40012)
                     /gene="frlA"
                     /locus_tag="OO130_000666"
     CDS             complement(38624..40012)
                     /gene="frlA"
                     /locus_tag="OO130_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
                     /protein_id="HCR5364185.1"
                     /translation="MTANSPLQRVGQEKGIAMGSQELQRKLGFWAVLAIAVGTTVGSG
                     IFVSVGEVAKAAGTPWLTVLTFVIGGLIVIPQMCVYAELSTAYPENGADYVYLKNAGS
                     RPLAFLSGWASFWANDAPSLSIMALAIVSNLGFLTPIDPLPGKFIAAGLIIAFMLLHL
                     RSVEGGAAFQTLITIAKIIPFTIVIGLGIFWFKAENFAAPTTTAIGATGSFMALLAGI
                     SATSWSYTGMASICYMTGEIKNPGKTMPRALIGSCLLVLVLYTLLALVISDLMPFDKL
                     ANSETPISDALTWIPALGSTAGIFVAITAMIVILGSLSSCVMYQPRLEYAMAKDNLFF
                     KCFGHVHPKYNTPDVSIILQGALGLFFIFVSDLTSLLGYFTLVMCFKNTLTFGSIIWC
                     RKRDDYKPLWRTPAFGLMTTLAIASSLILVASTFVWAPIPGLICAVIVIATGLPAYAF
                     WAKRSRQLNALS"
     gene            complement(40257..40424)
                     /locus_tag="OO130_000667"
     CDS             complement(40257..40424)
                     /locus_tag="OO130_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HCR5364186.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
BASE COUNT         9346 a        10255 c        11118 g         9709 t
ORIGIN      
        1 tgtggaataa aaaggccgga gatattctcc ggcactttta tgtcaggcaa ggcgttcgtt
       61 gccagatgcg gcgtgaacgc cttatcgcgc aggtttaggg gccagtccgg gcgtttaacg
      121 ttttttgccc attgccgccg ccagcgcatc catcatcgcg ctattaccgg caggctgcgc
      181 ttcacgaccg cgtggtttgg ctgccgggcg gttgttttgc gggcgttcat taccgccgcc
      241 gcgacgggcg ttggtttcgc caggctgctc gtccagacgc atagtcaggg cgatacgttt
      301 acgctgaaga tccacttcca gcactttcac cttcacaatg tcgcccgctt tcaccacggt
      361 atgcggatct tccacaaact tgttcgacaa tgaagagatg tgaaccaggc cgtcctgatg
      421 cacgccgata tcgacaaacg cgccaaagtt ggtgacgttg gtcaccgcac cttcgaggat
      481 catacccggt tgcaggtcgt tcattgtctc gacgccatcg gcaaactgag cggttttaaa
      541 ttccggacgc ggatcgcgac ccggtttttc cagctctttg atgatgtcag ttactgtcgg
      601 cacaccgaat ttttcatcag taaagtcaga cgctttcagg ttacgcagtt cgctgctgtt
      661 acccatcaga tctttcagtg cctgctgtgt tgctgccaga atgcgttcca ccaccggata
      721 ggcttccggg tggacggtag aagcatccag cgggttatca ccgtggttaa tgcgcaagaa
      781 gcccgcgcac tgctcgaagg ctttcggccc cagacggctg actttcagca gttgctgacg
      841 gttctggaac tggccgttct catcgcgcca ggccacgatg ttttgcgcca tcatgcgcgt
      901 caggcccgcc acgcgggtta acagcggaac agaagcagtg ttgagatcga cgccaacggc
      961 gtttacgcag tcttctacta ctgcgtccag tttgcgggcc agttgcgtct ggctgacgtc
     1021 atgctgatac tgacctacgc cgatagattt cggatcgatt ttcaccagct ccgccagcgg
     1081 atcctgcaaa cggcgggcga tagacaccgc gccacgcagc gaaacgtcga gatccgggaa
     1141 ctcctgcgct gccagctcgg aagctgagta aaccgacgcg ccagcttcgc tgacgatcac
     1201 tttctgtgcg gtcactttcg ggaactgctt ctgcacgtcg agatagaaac gttcagtttc
     1261 gcgggaagct gtaccgttgc cgatcgccac cagttcaacg ttatgttttt cacacaaggc
     1321 agcaacggtc atcgctgctt ttgcggcttg tccggtgtgc gggtaaatgg tatcggtcgc
     1381 taccagtttg ccagtggcat cgaccaccgc cacttttacc ccagtacgca gacccggatc
     1441 gaggcccatc gttgcacgca gtccggcagg ggccgccatc agcagatcgt gcaggttacg
     1501 ggcaaagacg ttgattgctt catcttccgc gcgttcgcgc acggtaccca tcagttcggt
     1561 ttccagatgc atcagcacct tgatgcgcca ggtccagctc actacgcctt tgcgccagct
     1621 atccgccggg gcattgttca ggcgcaggcc aaggtgatcc atgatgattt gctcgcaata
     1681 gctctctttg ggcggctcat cgaactgcgg atcggcattc agcgaaagct ggagtacgcc
     1741 ttcgttacgc ccacggaaca tcgccagagc gcggtgagaa ggcaccgtgg acaacggttc
     1801 gtgatgatcg aaatagtcgc ggaatttcgc cccttcctct tctttaccgc tcaccaccgt
     1861 agaaaccaaa tgcgcgttct tccacagata atcacgcact ttcgccagca gcgcggcatc
     1921 ttcggcaaac cgttccatca ggatatagcg cgcgccatcc agcgcggctt tggtatctgc
     1981 cacgccttta tcggcatcaa catattgtgc agcggcgact tctggcgtgt gtgacggatc
     2041 gctccacagc aggtcagcca acggctcaag ccctgcttca atggcgattt gcccgcgggt
     2101 gcggcgttta ggtttgtagg gcaggtagag gtcttcgagt tcggttttgc ttagggtggc
     2161 gttgatggcc ttcgccagat catcggtgag tttgccttgc tcggaaatgg acttgaggat
     2221 cgcctgacgt ctctcttcca gctcgcgcag atagctcaga cgcgtttcca gattacgcag
     2281 ctgcgtgtca tccagaccgc cggtgatttc cttacgataa cgtgtgataa acggcacggt
     2341 attcccttcg tcaagcaggc gaacggcagc gtcaacctgt tccgggcgcg cctgaatttc
     2401 acccgcaata atgcggcaga acgaatcatt catcatggtt tgagttcatc ttttcggatc
     2461 aaaaatcagg ggatagttat acggactggc tggcaaaaat gccagccatc ggcaggaggt
     2521 taagactctt ccttacggtt tcacgtactc gatagcatta acataccagc tcgcttcccc
     2581 ggcaggggta ttcaccaccg ccagatcgcc gacttctttt ttcagcaatg cgcgggccat
     2641 cggggaatcg atagagatgt aatctttacg gccaaaaatt tcatcgtagc cgacaatacg
     2701 gaaacggtga gtcacgccat cgtcgttttc aatctccacc cacgcgccaa aaaagacttt
     2761 gccttcctgc tgaggggagt aatcgacgat tttgagattt tccatgcatt tagtgagata
     2821 gcgcacgcga cggtcgattt cacgcagacg ctttttatta tactgatagt cagcattttc
     2881 gctgcggtcg cccagacttg cggcccaggt cacctttttt gtgacctccg ggcgttcttc
     2941 acgccagaga taattaagct cttgtttgag tttttcatac ccttcccggg taaccagggg
     3001 cgttttcatc tcgttgattc cctttgtctg tttgataatg cgcacattgg gtataacgtg
     3061 atcatatcaa cagaatcaat aatgtttcgc cgaataaatt gtatacttaa gctgctgttt
     3121 aatatgcttt gtaacaattt aggctgaaat tcataccaga tttagctggt gacgaacgtg
     3181 agctttttta agaatacacg cttacaaatt gttgcgaacc tttgggagta caaacaatgc
     3241 aagagaacta caagattctg gtggtcgatg acgacatgcg cctgcgtgcg ctgctggaac
     3301 gttatctcac cgaacaaggc ttccaggttc gaagcgtcgc taatgcagaa cagatggatc
     3361 gcctgctgac tcgtgaatct ttccatctta tggtactgga tttaatgtta cctggtgaag
     3421 atggcttgtc gatttgccga cgtcttcgta gtcagagcaa cccgatgccg atcattatgg
     3481 tgacggcgaa aggggaagaa gtggaccgta tcgtaggcct ggagattggc gctgacgact
     3541 acattccaaa accgtttaac ccgcgtgaac tgctggcccg tattcgtgcg gtgctgcgtc
     3601 gtcaggcgaa cgaactgcca ggcgcaccgt cacaggaaga ggcggtaatt gctttcggta
     3661 agttcaaact taacctcggt acgcgcgaaa tgttccgcga agatgagccg atgccgctca
     3721 ccagcggtga gtttgcggta ctgaaggcac tggtcagcca tccgcgtgag ccgctctccc
     3781 gcgataagct gatgaacctt gcccgtggtc gtgaatattc cgcaatggaa cgctccatcg
     3841 acgtgcagat ttcgcgtctg cgccgcatgg tagaagaaga tccagcgcat ccgcgttaca
     3901 ttcagaccgt ttggggcctg ggctacgtct ttgtaccgga cggctctaaa gcatgaggcg
     3961 attgcgcttc tcgccacgaa gttcatttgc ccgtacgtta ttgctcatcg tcaccttgct
     4021 gttcgccagc ctggtgacga cttatctggt ggtgctgaac ttcgcgattt tgccgagcct
     4081 ccagcagttt aataaagtcc tcgcgtacga agtgcgtatg ttgatgaccg acaaactgca
     4141 actggaggac ggcacgcagt tggttgtgcc tcccgctttc cgtcgggaga tctaccgtga
     4201 gctggggatc tctctctact ccaacgaggc tgccgaagag gcaggtctgc gttgggcgca
     4261 acactatgaa ttcttaagcc atcagatggc acagcaactg ggcggcccga cggaagtgcg
     4321 cgttgaggtc aacaaaagtt cgcctgtcgt ctggctgaaa acctggctgt cgcccaatat
     4381 ctgggtacgc gtgccgctga ccgaaattca tcagggcgat ttctctccgc tgttccgcta
     4441 tacgctggcg attatgctat tggcgatagg cggggcgtgg ctgtttattc gtatccagaa
     4501 ccgaccgttg gtcgatctcg aacatgctgc tttgcaggtc ggtaaaggga ttattccacc
     4561 gccgctgcgt gaatatggcg cgtccgaggt acgttcggtt acccgcgcct ttaaccatat
     4621 ggcggctggt gttaagcaac tggcggatga ccgcacgctg ctgatggcgg gggtaagtca
     4681 tgacttacgc acgccgctga cgcgtattcg cctggcgacc gagatgatga gcgaacagga
     4741 tggctacctg gccgaatcga tcaataaaga tatcgaagag tgcaacgcca tcattgagca
     4801 gtttatcgac tacctgcgca ccgggcagga gatgccgatg gaaatggcgg atctcaacgc
     4861 agtactcggt gaggttattg ctgccgaaag tggctatgag cgggaaattg aaaccgcgct
     4921 ttaccccggc agcattgaag tgaaaatgca cccgctgtcg atcaaacgcg cggtggcgaa
     4981 tatggtggtc aacgccgccc gttacggcaa tggctggatc aaagtcagca gcgggacgga
     5041 gccgaatcgc gcctggttcc aggtggaaga tgacggtccg ggaattgcgc cggaacaacg
     5101 taagcacctg ttccagccgt ttgtccgcgg cgacagtgca cgcaccatta gcggcacggg
     5161 attagggctg gcaattgtgc agcgtatcgt ggataaccat aacgggatgc tggagcttgg
     5221 caccagcgag cggggcgggc tttccattcg cgcctggctg ccagtgccgg taacgcgggc
     5281 gcagggcacg acaaaagaag ggtaaataaa cgggaggcga aggtgcctcc cgttttgctt
     5341 tctataagat actggataga tattctccag cttcaaatca ttacagtttc ggaccagccg
     5401 ctaccagcgc ggcacccgca ggggtgtcgg tgtatttatc gaagttgtcg ataaacagtt
     5461 tcgccagggt ttcggctttt tcctgccact gttccggaga agcgtaggtg ttacgcggat
     5521 cgagaatctt cgtgtctacg cccggcagtt cggttgggat cgccaggtta aacatcggca
     5581 gagtgaaggt ttctgcatta tccagcgaac cgttgaggat ggcgtcgata atggcgcggg
     5641 tatctttaat cgagatacgt ttgcctgtgc cgttccagcc agtgttaacc agataagcct
     5701 gcgcgcccgc cgcctgcata cgtttcacca gcacttctgc gtactgagtc gggtgcagcg
     5761 acaggaatgc cgcgccgaag caagcggaga aggttggcgt cggttcggtg atgccacgct
     5821 cagtaccggc cagtttggcg gtgaagccag agaggaagtg atactgggtt tgatcggcag
     5881 tcaggcgaga aaccggcggc aacacgccga aagcatcagc agtcaggaag ataaccttag
     5941 tcgcgtggcc cgctttggaa accggcttaa cgatgttatc gatgtgatag attggataag
     6001 aaacgcgggt gttctcggtt tttgaaccat catcaaagtc gatagtgcca tcttcacgca
     6061 cggtgacgtt ttccagcagc gcatcacgac ggatagcgtt gtagatttca ggttccgctt
     6121 ctttcgacag cttgatagtt tttgcgtagc agccgccttc gaaattaaac acgccgtcat
     6181 cgtcccagcc gtgttcgtca tcgccaatca ggcggcgttt cgggtcggtg gaaagggtgg
     6241 ttttaccggt gccggaaagg ccgaagaaca ccgcaacatc gcctttctcg ccaacgttgg
     6301 cggagcagtg catagaagcg atacctttca gcggcagcag gtagttcatc atcgagaaca
     6361 tccctttctt catttcgccg ccgtaccagg tgccgccaat cagctgcatg cgctcggtca
     6421 ggttaaacgc cacgaagttt tcggagttca gaccctgttc tttccactgc gggttagtgc
     6481 acttcgcgcc gttcataacg ataaagtctg gtttgaaacc tgccagctct tcatcgctcg
     6541 ggcgaataaa catgtttttg acaaaatgcg cctgccaggc cacttcggtg atgaaacgga
     6601 cggaaagacg agtatccggg ttcgcaccac agaaagcgtc gacaacgaac agacgtttgc
     6661 cggaaagctg cctggtcacc aggcctttca gatgctgcca ggtttccgga gagagaggtt
     6721 tgttgtcgtt cttacctttg cctttgtctg cccaccagaa agtatcgcga gtggtatcgt
     6781 cacggacgat atacttatct tttggtgaac gaccggtgaa gatcccggta tcgacggcaa
     6841 cggcacccag attagttaac accccgcgct cataacctgt caggctcgga tcgagctctt
     6901 cctgatacag caggtcgtag cttgggttgt aaacgatatc atgtacgtca ctgataccat
     6961 aagcctcgag ttcttgcggg gtcaaaccat tgttaacgcg catttcactg ctccttagcc
     7021 aatatgtatt gcctgaatag taaagtcttt ttgggggtgt taaccgcgac aaggctcata
     7081 gatttacgta tctggagaaa ttcatatttg acaaaaaacc ccgtgattcc tgttacgaaa
     7141 cggttgctat tatcgcagaa atagcatttt aggtgtggaa aatgttctca aaaggttaaa
     7201 tcttggttta tatggcggga atattgtgag tggaatcgca ttccttaatg aaattgatgc
     7261 aaattcaatc ggtaaagtat cgattcacca cgctaacttt ttttgattat tttctgaaaa
     7321 aagcaaaata ctactgcccg gatgcgataa ttaacccacc ttttgattct ggataaccaa
     7381 catggataac gttgaacttt caccggcaac acgctggggg atgattgcta ccggattact
     7441 tcaggggctg gtttgctacc tgttgatcgc ctggcttgcc ggaaaaaatc acagttggat
     7501 tgtttatggc gtgcctgcga ccgttgcgtt ttcatccgtt ttactcttct ccgtgatctc
     7561 ttttaaacag aagcgccttt ggggatggct ggcgctggtg tttattgcca cgacagggat
     7621 gagcggttgg ctgaagtggc aaactgatgg catgaacccc tggagagctg aaaaggcgat
     7681 ttgggatttt ggctgctatc tgctgttgat ggcaatgttg ttgctaccgt ggatacaaca
     7741 aagccttcgt atccgcaatt gcagtagccg ctatagctac ttttaccagt cagtatggca
     7801 taacgtactt atattactgg tgatttttct cgccaatggc ctgacgtggc tggtgctttt
     7861 actgtggggt gagttgttta aacttgttgg catcaagttt ttcaatacgc tcttttttgc
     7921 aaccgactgg tttatgtatc ttacattagg tctggttacc gcactggcgg tgattctcgc
     7981 gcgcacacag tcacgtttaa tcgactctat tcaaaagttg ttcacgttaa tcgccacggg
     8041 gttgctgccg ttagtatcat tgctaaccct gatgtttatc atcactctgc cgtttacggg
     8101 cctgagcgcg atttctcgcc acatctccgc cgccgggttg ctgttaacgc tggccttttt
     8161 gcaattgatc ttaatggcta ttgtccgcga tccgcaaaaa gcgtcacttc cctggacggg
     8221 gccgttgcgt tgcctgatta aaaccgcttt gctggttgct ccgctgtatg tgttcgtcgc
     8281 cgcctgggcg ttatggctgc gggtcgctca gtacggctgg actgtcgacc gcttgcaggg
     8341 cgcgctggcg gtgctggtgt tactggtgtg gtcgctgggg tattttgtca gtatcgtctg
     8401 gcgtaatggg caaaatcccc ttgttcttca gggcaaagtg aaccttgcgg tttcgttatt
     8461 ggtgttggtg atactggtgc ttcttaattc gccggtgctg gacagtatgc gtattagcgt
     8521 gaacagtcat atggcgcgtt atcagagcgg caaaaacacg ccagaccagg taactatcta
     8581 catgctcgaa cagagcggtc gctatggacg tgcggcgctt gagtcgctga aaagcgatgc
     8641 cgggtttatg aaagacccga aacgcgcgcg ggatctgctg atggcgttag atggagagca
     8701 acatcttcag gaacaggtat cggaaaaagt attagccgaa aatgtgttaa ttgcccctgg
     8761 ttctgttaaa ccggatgtga cattctggtc ggccttaatc caggatcgct ataacgtgat
     8821 gacctgtatt gaaaaagacg cctgcgtcct ggtcgagcaa gatctgaata gtgatggtca
     8881 ggcggagcgg atcctgtttg cttttaatga tgacagagtc attgtctatg gctttgactc
     8941 agacagaaaa gaatgggacg cgcttgatat gagtttactt ccgaacgaaa taacgaaaga
     9001 aaaattactc acagctgcga aggatggaaa actggggacg aggccgaaag cgtggcgcga
     9061 tctgacggta gatggtgaaa cgctggaaat aaacttaagt aagtgacaac gcttttatcg
     9121 gatacgacgc tgtatctgac caaaataatt tgcgatttat ttcataggcg ctggtaagcg
     9181 cagcacatca ggccattaag ttgtaaaatc cggcagagcc ctctctgccg gacatactca
     9241 ttaatgaact tgcggatctg ccggagacgc gttgttgcgg atttcagcaa tatccatcgc
     9301 attgaacaga tagtggttac cgcagtaatc acaatgcatg tcaatttcgc catcttccgc
     9361 caggatgcta tcaacttctt catcaggcag cgttttcagc gcatcggcgc aatgttcacg
     9421 cgagcaggtg catttgaact ccacatcctg cggatcgtaa accgtcacct cttcttcgtg
     9481 atacaaacgc cacaacactt cgtttgccgg taaggtcagc agttcttcgg ttttgatggt
     9541 ttcggttagc gtcgccaggt ggtcaaagtc gtcctgctgg gcattttgcg caggcattac
     9601 ctgcaacaac ataccgcctg cagccggttt gccgtctacg tcgccggtgc gaataaacag
     9661 gcgcgtcggc agctgttcag aacgcataaa gtaatcttcc aggcaggccg ccagggtatc
     9721 accttccaga ccaactacgc cctgatagcg ttcgccttcg ctcggggtaa tggtgatcac
     9781 cacgtaacca ttgccgacca gcgttttcag gtcggcattt tctggaattt cgccctgcac
     9841 gcgcgccaca ccgcgcattt gctggttatt gttaccgtta ataaccgcca gattcatcgg
     9901 accgtcgccc tgcagctgta cggtgatatc accatcaaac ttcagcgtag cggttaacag
     9961 gctggtcgca accagcagtt ctgccagcac gtttttaacg ggctgcggat aatcgtggtt
    10021 ctcaaggatc tgttgcaggg tttccgaaac ggttaccagt tcgccgcgaa cggcaaaatt
    10081 ttcaaacaga tagcgatgta attggtcatg ttgcggcata atcatctctc ttgcaggtga
    10141 cagttattca ctgtcgccgt gtttaaatcg taacaggtcg cggcgctctt ttttgtccgg
    10201 gcgtcggtcc gggtgcggca tggttaaggc attaagttta cgtgccatcg ccattttttc
    10261 gcgtttctct acactttccg cagtctcttc atacagcaag gctgcctcgc tggcggggcg
    10321 acgctgttca gtaatcgcct ttacaatcac cgtgcgttcg tcatttccct ggcgcagagt
    10381 gagcgtggca ttcagctcga cgattttgct cggcttgctg cgctgcccgt tgtaatgcac
    10441 cttaccgcct tcaatcattt cacgggctag cgcgcgggtt ttataaaaac gggcagccca
    10501 tagccattta tccagtcgaa cctcaacagc aggtttctct ttcatggcgt ctccttcaca
    10561 ttagcgaggg gatcaggcgg cggtagtcat tcagtgacgg atggcgttga tactgtttct
    10621 cggcaatccc ggaatcagga ttagtcacgc cgaggcagta acgaatacca aattgcgcgg
    10681 cagcatcgag aatcgcttcg ctgtcatcaa taaacagcgt tctttcagct ttcagacccg
    10741 tagcttcggc caccgcatgc cataaccgct gatcctcttt cggataacca aatgtgtggg
    10801 tggaaagtaa taaatcaagg tgtgcgtcca gacccgtatg ctcaagtttt accgccaggt
    10861 tgtgcggatg agcattggtg agcaaaattc gctgcttacc gctggctttc agtgcctcaa
    10921 gaaacggaat ggtatcttca cgcagtacgg cgcgcggtcc catctcggtg gtcattgcac
    10981 agatatccag acccagttgc tcactccagt aatcaagaca gtaccagttt agcgtatgct
    11041 gtacgtcgtg atattgctgg cgcatatatt ccatcgcttc ctgtggcgta accccgtttt
    11101 tcgcgcccca tgtttcaggc accagctttt gccagaaata gttatcgaag gcgaggtcga
    11161 gcaacgtgcc gtccatatcc agcagaacgg tatctacgtc ctgccaggca atgttgatat
    11221 gcatgaggga aatctccaga gtgaagcaat ttgcgcgaca gggtagcata acctgtcgcg
    11281 caaacgtgtt attcgataag gctttctgaa ggggtgatca gttgcgggtt caggcagctt
    11341 tcataatagg cctgaatctc catcatgcga gtgcgatgac gctggtaacg acgccaggcc
    11401 tgtacgccat tgtaaatggc gctacccagc atcgtcagta gcagcaatgt ggtgctgaga
    11461 tagcgccaca ggccggaacg atccgggatc ggatgaaggc caatatgctg cgtaccattg
    11521 gcgtcagtga agatttttgt gacgatacct tcggcgttaa acggcgtatg catcagcatt
    11581 tgtgccagtt tctggaaagc gttccactgt tcttgcggcg ggtagtcgta aagtgatgcc
    11641 gaaggccagg gctgatcaac aaaatcgctg ccttcgtcgc tgacaatcag gaatccgccg
    11701 ggggccggac tgtttagtga ttgtgccgct cgcgccgttt catgcgtgat aaacggcgcg
    11761 gtggaggttg ccaccaggtt atccagcgat tccgcactca ccgggcgtaa taacacattc
    11821 acgccatcga gcttcccggc gttggcgcgt tttaccagcg cgtcccagtc tttactgttg
    11881 ccgagattga ccagcgcatt tttcagtcgc acacagtcat ctttggcaga acataaatcc
    11941 gctgtcttca gtacaatgtc gccaaaatca tcaagcaata ccatgccgga tttttgaatc
    12001 gctgaacgta atgaggcact gacgcgagat tcatcttccg gtttagggtg cagctggcga
    12061 ttaactgctt cagtcaatgc cgtcgctttg ttgaccagtt cagattctgg taatggtaat
    12121 gagcgggcgt cattccagat gatctgcgag cagtcaaacg gtaaaaaagg tgaattggtt
    12181 ttcgcgctcc aggttccgga agtgcgaata ttacacattc ccgtaccgct aatacgcaat
    12241 gtatcgccta cccgcacgcc agcgtcagcc agttgtttta cgctggtggc ttcaatggtc
    12301 tgcgcgcctt tcatccatga gagagtgaat ttcagcggca tatccagcgg gatccagaat
    12361 aacagcataa acagcaccag cagcgagccc gcagcgataa tcgtactgcg cagccagtgc
    12421 tgtaacggaa agttttttac ttcatcatgc agcgaaagat atcgcccctg acgcactacg
    12481 tggcagtcga gatagatatc aatatcggtt tgttgatcga gatcctgagc aatgtatggc
    12541 tgccagtgtg cgggatagac caggtcgata ataccgagtg aaatattgtt gatctgttcc
    12601 tgatcgtttt cgccaaacaa tccccaacga cggggtgtac cgcgcagaca atgaatttcc
    12661 cgcagggagg attttgccgg gggcgcgaat agcccccaca gacctgcgcc cagcagcagt
    12721 aatgcgccgc ctgccagcca cggaacaaat acatccgggg taatcaggca gaaaaagaac
    12781 atcaggaagg aggcgacgat cagcaacgct tcacgcagcc cgcgcggacg actcagcgca
    12841 tattcttcat gcgtttcttt gcgaatattg agtagctcga tttgctcact ctcttccccg
    12901 cgaatcgacg cctgcgtgga cggaacaggt tgcaaggcat agccgcgcgt ttcctgcatg
    12961 tactcctgta gcgtatgacc gttgagtgaa ataaccaacg gcagcgaatc ggtatgaatc
    13021 agttcaacgg tattctcatc gttgatgtac tgttcccaga aggggggcag gtggacttct
    13081 accgaatcga ggtagtaacg ccatttattc ggatcgtcgg tagagatgcc gtaacgtgtg
    13141 atagcgtgtg tcagcatcat gacgttgttg ctttcggcat tcagcgccag agagatcggt
    13201 gccgcgctag ctcccgttgg gccaggcacc tgtaatacct gcgtcaggct ctcaagataa
    13261 ttttcaacgg cgctacgttc ttcaggtgtg agtttacgcg tttgcgcatc cgcgaaggcg
    13321 ttggtccagg gcagctgact ccgtctggat cgcactttta tcagccatcc cgcaagtagt
    13381 gagcaggcca gcaaagcagc taaaaaaatc acaatggtgc tcatgctttc cccatcttac
    13441 ttagtctgtc aggcgtggtc agtcgcacca gacacaatac atgattctgc ggttggctat
    13501 cggcaagcgt ggagtttaac ttgagcatca ttaagcacat cacagtaacc gctgatcata
    13561 gcaaaccctc tgaaggcgga atatcagcaa attcctagag ttatttcaac ctattgactt
    13621 ttaatttgat ttgaggacga ttatttatta agttacataa atgtaaataa catgcaattc
    13681 acattgccga aagcgtgtcc gatatcgcac aataaccgct gatttcacag catagatccc
    13741 acgatgagca aatcattaca aaaacccacc attctgaatg ttgaaactgt agcccgttcc
    13801 cgactgttta ccgtcgagag cgtggatctg gagttcagca atggcgtgcg gcgtgtttat
    13861 gaacgaatgc gtccaaccaa ccgggaagca gtgatgattg tgccgattgt ggacgatcac
    13921 ctgatcctga tccgcgaata cgcagtggga actgaatcct acgaattagg tttttcgaaa
    13981 ggattaattg atccgggtga aagcgtctac gaagccgcta accgcgagct aaaagaagag
    14041 gttggatttg gtgcgaacga tctgactttt ttgaagaagc tcagcatggc accgtcttac
    14101 ttttccagca aaatgaatat cgtggtcgcg caagatctct acccggaatc gctggaaggc
    14161 gatgagccag agccgctacc acaggtgcgc tggccgctgg cgcatatgat ggatttgctg
    14221 gaagaccctg acttcaacga agcgcgtaat gtcagcgcgc tgttcctcgt gcgcgaatgg
    14281 ttgaaagggc aggggcgagt gtaaatgcgt gaagtttgcc tgatgcacaa cgcttatcag
    14341 gcttatgggg ttcctgcaat atattgattt ttcacgattt tgttagccgg gtaacgcggt
    14401 cacccgcatc cagcataaac aaatgcactt tgtcagccaa ctgaaaaggc gccgaagcgc
    14461 ctttttaatc agaacaattc ctgtgcctca ccattatcga taatggtcgt tcccacctcg
    14521 tgcaccgcct gttgtgtcgg ctgcgtacct tcgatgaaat actcttcgcg gctgttgcca
    14581 ccattagcta actgcccggt gctgcgatcg atattcaccg tcacaatacc cggtggcggc
    14641 gtcagcggct gctccggcac accttcaaga acggctttca tataagcgtc ccatgcaggc
    14701 tgggcactct tggctccgcc ttcgtaacct gagatctgat ctttaatcgc tccggaagcc
    14761 gttgtatgac cgagattacg acggtgatca tcaaagccaa tccagaccga ggtcacaacg
    14821 cccggaccgt aacccgagaa ccacgcatct ttcgaactgt tagtggtacc ggttttcccg
    14881 ccgatatcgc gacgctgcaa atcacgacct gcacgccagc cagtaccctg ccagcctggc
    14941 tcaccaaaga tattggtgtt caaagcactc ttaatcagga atgccagcgg agtgttgatg
    15001 acgtgcggtg cgtactcctg cgcgccagtc ttcgccacta acgcctgatt tgcctgctcc
    15061 agctgcggca ttggtacaga aacattctgc tgctcgcggg agatagcgac atcttcaaca
    15121 tcgttatttt ccagcacgtt cgatttctgc gtatcaccgt aaatcaccgg aatatcgcat
    15181 tccgggcagg ctactttcgg tttcgcttcg aaaatcacgc cgccctgatc gttttcaatt
    15241 ttgctgataa accacgggtc caccaggaag ccgccgttcg ccatgaccgc gtagccgcgc
    15301 gccacctgca ttggggtgaa ggacgctgaa cccagcgcca gcgattcggt gtggacaatg
    15361 ttttgcgccg ggaagccgaa gcgttgcaga tattctgcag cgtagtcgac gcccatagcc
    15421 cgcattgcac gtaccatcac cacgtttttc gactgaccca gcccctgacg taagcgaatt
    15481 ggaccagcat attgtggcgg cgagtttttc ggctgccagt cagaaccggc acctgcgtcc
    15541 cagcgagaaa ttggcacatc gttcaacata cttgccagcg tcagaccttt atccatcgcc
    15601 gcggtgtaga ggaacggttt gatgttggaa cccacctgac gcagtgcctg ggtggcgcgg
    15661 ttaaacttgc tctgattgaa atcaaagcca ccgaccagcg ccataacggc accgttttgc
    15721 ggattgatcg acaccagcgc cgagttcact tccggcactt gtgccagcca ccatgcatcg
    15781 ccaacctgac gaacccagat ttgctgaccc gtttgcagaa catcggtcac tttacgcggc
    15841 gtcggtccct gctgagtatc cgaacggtaa ggacgcgccc agcgaacgcc ttccatactc
    15901 aatgcgacgg tcgacccgtc cgccagcatc gccgtcgctc cctgaggatt ggcgctggtg
    15961 actgcggcag gcagcagcgg accataggtc ggcagcgcct tcagcgtatc ggtaatcttg
    16021 ttgttatccc acgccgactc gcccactttc cacagcacat ttgccgggcc gcgatagccg
    16081 tggcgcatgt cgtagtccag cacgttatta cgtaccgcct gctgtgcggc ctgctgcact
    16141 ttgcgggtga tggtggtgta aatgcgataa ccgtcttcat aggcactttc gccataacgg
    16201 ttatacatct cctggcgcac catttcgctc aggtacggcg cagagaaagc aatctccggc
    16261 gcgtgatagt tagcgttaat cgcctcagtg cgtgtctgat cgaactgttg ttgggtgata
    16321 tacccttcat ccagcatccg cgacagtacg acgttacgcc gcgcgacggc acgatccatt
    16381 gagtagagcg gattaaaggt ggaaggcgct ttcggcagcc cggctatcac agccatttcg
    16441 ttcagcgtca gttggtcgac cgttttaccg aaatagactt gtgccgcagc accgacacca
    16501 taggcgcggt aaccgaggta aatcttattc agataaagct cgaggatctc gtctttcgtc
    16561 agcaactgtt caatgcgaat cgcgaggaag acttccttaa tcttacgcat cagcgtgcgt
    16621 tctggactga ggaagaagtt tctcgccagc tgctgggtaa tggtactcgc cccttgtgac
    16681 gcgtggccgg agaacagcgc cacacttgcc gcacggaaga tccccaccgg gtcaacgccg
    16741 tgatgctcgt agaagcggct gtcttccgtc gcgataaagg ctttcaccat ctccggcggg
    16801 atttgatcca acgtaaccgg aatacgacgt ttctcaccgt attgagcaat cagctcgcca
    16861 tcggcgctgt aaatctgcat cggaatttgc aggcgaacat cttttaatgt cgccacatcc
    16921 ggcagttgtg gctcgatgta gcggtatagg ccataaatcg agcctgctcc cagcagaatg
    16981 caacagactg caaggatcaa aaaatacttt acgaacttca ctggaaattt cccatttagt
    17041 ttcatttggg cagtttataa acaaacgcgc gttagtataa aggcaagcca gacgcattga
    17101 tatacccgtc agagtgacgg gtgataagga gatcatcaca atggcattta agatctggca
    17161 aattggtttg catttacaac agcaagaagc ggtagcggtt gcgatcgtgc ggggcgcaaa
    17221 agaatgcttt ttgcaacgct ggtggcggtt gccgctggag aacgacatta tcaaagatgg
    17281 gcggattgtt gatgcgcagc agctggctaa aacgttgtta ccttggagtc gcgaactgcc
    17341 gcagcgtcat cacattatgt tggcgtttcc cgccagtcgc acattacagc ggtcatttcc
    17401 gcgcccgtcg atgtcccttg gtgagcggga gcaaacggcc tggctgtcag ggacgatggc
    17461 ccgcgagctg gatatggatc cggactccct gcgcttcgat tacagcgaag actcactcag
    17521 tcccgcctat aacgtgactg ccgcgcaaag caaagagctg gcaacgctgc ttactctggc
    17581 agaaaggttg cgtgttcatg ttagtgcgat caccccggat gccagtgcat tacagcgatt
    17641 cctgcctttt ttaccttctc atcagcaatg tctggcctgg cgtgataacg aacagtggct
    17701 gtgggcgacg cgctatagct gggggcgcaa actggcggtg gggatgacta gcgcgaagga
    17761 gctggcggca gcgttattcg ttgatcccga cagcgtcgcg atatgtggcg aaggcggatt
    17821 tgacccctgg gaggccgttt ctgttcgtca gccgctgcta ccgccgcctg gtggagactt
    17881 tgccatcgcg ctggggctgg cgcttgggaa ggcgtactga tgaacccgcc aattaatttt
    17941 ttgccctggc gacagcaacg ccggaccgct tttctgcgtt tctggttgct gatgttcgtt
    18001 gcgcctctgc tgctggccgt cgggataacg ctaatactgc gtctgacagg cagcgccgaa
    18061 gcgcgcatag acgcagtttt gcttcaggcg gaacaacaac tcgcccgcag cttacagata
    18121 acgaagccac gtttgctgga gcaacagcag ttacgcaaac agcgttcgca acggcagcgc
    18181 cagcgacaat ttacccgcga ctggcaatct gcgctggaag cactggcggc gcttttaccc
    18241 gagcacgcct ggctgacaac gataagctgg cagcagggaa cgctggagat caaggggctg
    18301 acaacaagca ttaccgcgtt aaacgcacta gaaacgtcac tccgccagga tgcgtctttt
    18361 catctcaatc agcggggagc cacgcagcag gatgcgcagg gacgctggca atttgagtat
    18421 cagttaacaa ggaaggttag cgatgaacat gttctttgac tggtggttcg ccacatcacc
    18481 ccgcctccgc cagttttgct gggcattctg gttgctgatg ttagttacgc tcatttttct
    18541 gtcatcgaca caccatgaag agcgcgacgc attaattcga ttacgggcaa gtcatcacca
    18601 gcagtgggcc acactgtatc gcctggttga caccactccc ttcagcgaag aaaaaacgct
    18661 gcccttttcg ccactggatt ttcagttgcc cggcgcgcaa ctggtttcct ggcatccatc
    18721 cgcgcaggga ggtgagttgg cgttgaaaac gctgtgggaa gcagtgccgt cggcattcac
    18781 acggctggca gagcgcaacg tcagcgtgag ccgtttttcg ttaagcgtgg aaggtgatga
    18841 tcttttgttc acgctacaac tggagacgcc gcatgagggt taaacgctgg ttgttggcag
    18901 gtattgcatt gtgcctttta accggtatgc gtgacccttt taaaccgccg gaagatctat
    18961 gccggattag cgaacttagc cagtggcgct atcaggggat ggtagggcga ggcgagcgca
    19021 tcatcggtgt aataaaagac gggcaaaaga aatggcgacg ggtgcagcaa aacgatgtgc
    19081 tggaaaacgg ctggacaatt ttacagctga cgccagacgt actaacgctg ggtaccggga
    19141 caaactgcga accgccacaa tggttgtggc aacggcaagg agatacaaat gaagcaatgg
    19201 atagccgcac tactgttgat gctgataccc ggcgtacagg cggcaaagcc gcaaaaagtg
    19261 acgctgatgg tggatgacgt tccggtagct caggtgttgc aggcgctggc tgaacaggag
    19321 aagttgaacc tggtcgtgtc gccagacgtc agcggtacgg tgtcgttaca tctaacagat
    19381 gttccctgga agcaggcact acaaactgta gtgaaaagcg ccggactgat aacgcggcag
    19441 gaaggcaaca ttctctcagt gcattccatt gcctggcaga ataacaatat cgcccgccag
    19501 gaggcggagc aggcgcgggc gcaggcaaat ctgccgctgg aaaatcgcag tataaccctg
    19561 caatacgccg acgcgggaga actggcgaaa gcgggggaga agctactgag tgccaaaggg
    19621 agtatgaccg tcgataaacg caccaatcgc cttttgctac gagataacaa aacggcgtta
    19681 agcgcgcttg aacagtgggt agcgcaaatg gatctgccgg tcgggcaggt tgagctgtcg
    19741 gcgcatattg tcaccattaa tgaaaaaagt ttgcgtgagt taggcgtgaa atggacgctg
    19801 gccgatgcgc aacacgctgg tggcgttggg caagtcacca cgcttggtag cgacctctcc
    19861 gtagcgacgg cgacaacgca tgtcggtttt aacattgggc gcatcaacgg acgcttgctg
    19921 gatcttgagc tttccgcgct cgaacaaaaa cagcagctgg atattatcgc cagtccgcgt
    19981 ctgctggcct cacatcttca gcctgccagc attaaacagg ggagcgaaat tccatatcag
    20041 gtttccagcg gggaaagtgg cgcgacgtcg gtggaattta aagaggccgt cctggggata
    20101 gaggtcacgc ccacggtgtt acaaaaaggt cgcatccggc tgaaattaca catcagccag
    20161 aacgttccgg ggcaggtgct acagcaggcc gatggcgaag tgctggcgat tgataagcag
    20221 gagatcgaaa cgcaggtcga ggtcaaaagc ggagaaacgt tggcgctggg cggcattttt
    20281 acccgtaaaa ataaatcggg tcaggatagc gtaccgttgc ttggcgacat tccctggttc
    20341 gggcaattat ttcgtcatga cggaaaagaa tatgaacgac gcgagttagt ggtgtttatc
    20401 acgccacgac tggtttccag tgagtaaaca gccgtaaaag cggtaatgtt tttatgctga
    20461 acgtgtttca tctatttgac gcgcgcaggt atttagcata caaggagtac cgatttgaga
    20521 gttggcgctc ttcgctgcct gcgttccatg atgatgattt atcattcagg cggcattttg
    20581 ctgtcttttt tacgctaatc ttacccggtg atttatcgcc agagcggtgg tagcaaggca
    20641 gcgcgcttgc agcgaccaga tatgcagagg gatgggtgat ttattcagtt gccaaacccg
    20701 ctggagtatt gagataattt tcagtctgac tctcgcaata tcttatgagg tttcagttca
    20761 tgtcctgcgg cgctctctga gcgaagcggg tttatcatta acgaatagtc ttagtagtac
    20821 cgaaaaaatg gcagagaaac gcaatatctt tctggttggg cctatgggtg ccggaaaaag
    20881 cactattggg cgccagttag ctcaacaact caatatggaa ttttacgatt ccgatcaaga
    20941 gattgagaaa cgaaccggag ctgatgtggg ctgggttttc gatttagaag gcgaagaagg
    21001 cttccgcgat cgcgaagaaa aggtcatcaa tgagttgacc gagaaacagg gtattgtgct
    21061 ggctactggc ggcggctctg tgaaatcccg tgaaacgcgt aaccgtcttt ccgctcgtgg
    21121 cgttgtcgtt tatcttgaaa cgaccatcga aaagcaactt gcacgcacgc agcgtgataa
    21181 aaaacgcccg ttgctgcacg ttgaaacacc gccgcgtgaa gttctggaag cgttggccaa
    21241 tgaacgcaat ccgctgtatg aagagattgc cgacgtgacc attcgtactg atgatcaaag
    21301 cgctaaagtg gttgcaaacc agattattca catgctggaa agcaactaat tctggcttta
    21361 tatacactcg tctgcgggta cagtaattaa ggtggatgtc gcgttatgga gaggattgtc
    21421 gttactctcg gggaacgtag ttacccaatt accatcgcat ctggtttgtt taatgaacca
    21481 gcttcattct taccgctgaa atcgggcgag caggtcatgt tggtcaccaa cgaaaccctg
    21541 gctcctctgt atctcgataa ggtccgcggc gtacttgaac aggcgggtgt taacgtcgat
    21601 agcgttatcc tccctgacgg cgagcagtat aaaagcctgg ctgtactcga taccgtcttt
    21661 acggcgttgt tacaaaagcc gcatggtcgc gatactacgc tggtggcgct tggcggcggc
    21721 gtagtgggcg atctgaccgg cttcgcggcg gcgagttatc agcgcggtgt tcgtttcatt
    21781 caagtcccga cgacgttact gtcgcaggtc gattcctccg ttggcggcaa aactgcggtc
    21841 aaccatcccc tcggtaaaaa catgattggc gcgttctacc agcctgcttc agtggtggtg
    21901 gatctcgact gtctgaaaac gcttcccccg cgtgagttag cgtcggggct ggcagaagtc
    21961 atcaaatacg gcattattct tgacggtgcg ttttttaact ggctggaaga gaatctggat
    22021 gcgttgttgc gtctggacgg tccggcaatg gcgtactgta ttcgccgttg ttgtgaactg
    22081 aaggcagaag ttgtcgccgc cgacgagcgc gaaaccgggt tacgtgcttt actgaatctg
    22141 ggacacacct ttggtcatgc cattgaagct gaaatggggt atggcaattg gttacatggt
    22201 gaagcggtcg ctgcgggtat ggtgatggcg gcgcggacgt cggaacgtct cgggcagttt
    22261 agttctgccg aaacgcagcg tattataacc ctgctcaagc gggctgggtt accggtcaat
    22321 gggccgcgcg aaatgtccgc gcaggcgtat ttaccgcata tgctgcgtga caagaaagtc
    22381 cttgcgggag agatacgctt aattcttccg ttggcaattg gtaagagtga agttcgcagc
    22441 ggcgtttcgc acgagcttgt tcttaacgcc attgccgatt gtcaatcagc gtaacaacaa
    22501 gaaaggtcag gccgcttatc aagcggtcta ttagcttcag gttaattgca acgtggtaag
    22561 cattaacctt ttagtggggt gttaaatgga tgaattcaaa ccagaagacg agctgaaacc
    22621 cgatcccagc gatcgtcgta ctggtcgttc tcgtcaatct tctgaacgtt ctgagcgtac
    22681 tgaacgtggc gaaccgcaga tcaattttga tgatattgaa cttgatgaca ctgacgatcg
    22741 ccgtccgact cgtgcgcaaa aagagcgcaa tgaggaaccg gaaatcgaag aagaaattga
    22801 cgaatccgaa gatgaaaccg tggatgaaga gcgcgtagag cgtcgtccgc gtaagcgcaa
    22861 aaaagcagcc agtaaacccg cttctcgtca gtatatgatg atgggcgtcg gcattctggt
    22921 tctactgctg ttgatcatcg gtatcggttc tgcgctaaaa gccccctcga cctcttccag
    22981 cgatcaaacc gcgtctggcg ataagagtat tgatcttgca ggcaatgcga ccgatcaggc
    23041 gaatggggtg cagccagcgc cgggaaccac gtctgcggaa aatactcagc aggatgtttc
    23101 tctgccaccg atctcttcta cgccgactca agggcaaacc ccggcggcaa cggatggtca
    23161 acaacgtgtt gaagtgcagg gtgacctgaa caatgcgctg actcagccac aaaatcagca
    23221 acagttgaac aatgtggcgg tcaattccac attgccgacc gaacccgcga ctgtcgcgcc
    23281 tgttcgcaat ggcaatgcat cgcgtgacac ggcgaaaacg caaaccgctg aacgtccgtc
    23341 cactacgcgc ccagctcgtc agcaggcggt gattgaaccg aaaaaaccgc aagcaaccgt
    23401 gaaaacggag ccgaagccgg tagcacagac gccgaagcgt actgaaccag ctgcccctgt
    23461 ggcgagcacg aaggcaccgg ctgcgacttc tacgccagca ccaaaagaga cggcgactac
    23521 ggctccagta cagacggcat ccccggcgca aaccacggca acaccagccg ctggagggaa
    23581 gaccgcaggt aatgttggtt cgttgaaatc ggcaccgtcc agccattaca ctctgcagct
    23641 gagcagttcc tctaactacg acaacctgaa cggttgggcg aagaaagaga atctgaaaaa
    23701 ctacgttgtc tatgaaacga cgcgtaatgg tcagccgtgg tatgtcctgg tttctggcgt
    23761 gtacgcttcg aaagaagagg cgaaaaaagc ggtatctaca ttgccagcag atgttcaggc
    23821 caaaaacccg tgggcgaaac cgctgcgtca ggtacaggcc gatctgaagt aatcaaggtt
    23881 atctcccgca atggtttatc gttgcgggag ctgcctgaag cgctggatgc tgtcggagct
    23941 ttctccacag ccggagaagg tgtaattagt tagtcagcat gaagaaaaat cgcgcttttt
    24001 tgaagtgggc agggggcaag tatcccctgc ttgatgatat taaacggcat ttgcccaagg
    24061 gcgaatgtct ggttgagcct tttgtaggtg ccgggtcggt gtttctcaac accgactttt
    24121 ctcgttatat ccttgccgat atcaatagcg acctgatcag tctctataac attgtgaaga
    24181 tgcgtactga tgagtacgta caggccgcac gcgagctgtt tgttcccgaa acaaattgcg
    24241 ccgaggttta ctatcagttc cgcgaagagt tcaacaaaag ccaggatccg ttccgtcggg
    24301 cggtactgtt tttatatttg aaccgctacg gttacaacgg cctgtgtcgt tacaatctgc
    24361 gcggtgagtt taacgtgccg tttggccgct acaaaaaacc ctatttcccg gaagcagagt
    24421 tgtatcactt cgctgaaaaa gcgcagaatg cctttttcta ttgtgagtct tacgccgata
    24481 gcatggcgcg cgcagatgat gcatccgtcg tctattgcga tccgccttat gcaccgctgt
    24541 ctgcgaccgc caactttacg gcgtatcaca caaacagttt tacgcttgaa caacaagcgc
    24601 atctggcgga gatcgccgaa ggtctggttg agcgccatat tccagtgctg atctccaatc
    24661 acgatacgat gttaacgcgt gagtggtatc agcgcgcaaa attgcatgtc gtcaaagttc
    24721 gacgcagtat aagcagcaac ggcggcacac gtaaaaaggt ggacgaactg ctggctttgt
    24781 acaaaccagg agtcgtttca cccgcgaaaa aataattctc aaggagaagc ggatgaaaca
    24841 gtatttgatt gccccctcaa ttctgtcggc tgattttgcc cgcctgggtg aagataccgc
    24901 aaaagccctg gcagctggcg ctgatgtcgt gcattttgac gtcatggata accactatgt
    24961 tcccaatctg acgattgggc caatggtgct gaaatccttg cgtaactatg gcattaccgc
    25021 ccctatcgat gtacatctga tggtgaaacc cgtcgatcgc attgtgcctg atttcgctgc
    25081 cgctggtgcc agcatcatta cctttcatcc agaagcctcc gagcatgttg accgcacgct
    25141 gcaactgatt aaagaaaatg gctgtaaagc gggtctggta tttaacccgg cgacacctct
    25201 gagctatctg gattacgtga tggataagct ggatgtgatc ctgctgatgt ctgttaaccc
    25261 aggttttggc ggtcagtcgt tcattccaca aacactggat aaactgcgcg aagtacgtcg
    25321 tcgtatcgac gagtctggtt ttgacattcg actggaagtg gacggtggcg tgaaggtgaa
    25381 caacattggc gaaatcgctg cggcgggcgc ggatatgttc gtcgccggtt cggcaatctt
    25441 cgaccagcca gactacaaaa aagtcattga tgaaatgcgc agtgaactgg caaaggtaag
    25501 tcatgaataa gtttgaagat attcgcggcg tcgcttttga tctcgacggt acgctggttg
    25561 acagtgctcc cggtcttgct gctgcggtag atatggcgct gtatgcgctg gagttacccg
    25621 tcgcaggtga agaacgcgtt attacctgga ttggtaacgg cgcagatgtt ctgatggagc
    25681 gtgcattggc ctgggcgcgt caggaacgtg cgactctgcg taaaacaatg ggtaaaccgc
    25741 ccgttgatga cgacattccg gctgaagaac aggtacgtat tctgcgtaaa ctgttcgatc
    25801 gctactatag cgaggttgcc gaagagggga cgtttttgtt cccgcacgtt gccgatacgt
    25861 tgggcgcgtt gcaggctaaa ggcctgccgc taggcctggt caccaacaaa ccgacgccgt
    25921 tcgtcgcgcc actgctcgaa gccttagata ttgccaaata ctttagcgtg gttatcggcg
    25981 gcgatgatgt gcaaaacaaa aaaccgcatc cggacccgct gttactggtg gctgagcgga
    26041 tgggaattgc cccacaacag atgctgtttg tcggcgactc acgcaatgat attcaggcgg
    26101 caaaagcggc aggttgccca tcagttggct taacctacgg atataactac ggcgaggcta
    26161 tcgatctcag ccagcctgat gtaatttatc agtctataaa tgaccttctg cccgcattag
    26221 ggcttccgca tagcgaaaat caggaatcga aaaatgacta agcccatcgt ttttagtggc
    26281 gcacagccct caggtgaatt gaccattggt aactacatgg gtgcgctgcg tcagtgggta
    26341 aacatgcagg atgactacca ttgcatttac tgtatcgttg accaacacgc gatcaccgtg
    26401 cgccaggatg cacagaagct gcgtaaagcg acgctggata cgctggcctt gtatctggct
    26461 tgtggtatcg atcctgagaa aagcaccatt tttgttcagt cccacgtacc agaacatgcg
    26521 cagttaggct gggcactgaa ctgctatacc tacttcggcg aactgagccg catgacccag
    26581 tttaaagata aatctgcgcg ttatgccgag aacatcaacg ctggtctgtt tgactatccg
    26641 gtgctgatgg ctgcggacat cctgctgtat caaactaatc tggtaccggt gggtgaagac
    26701 cagaaacagc acctggaact gagtcgcgat atcgcccagc gtttcaacgc gctgtatggc
    26761 gagatcttta aggtgccgga gccgtttatt ccgaaatctg gcgcgcgcgt aatgtcgctg
    26821 ctggagccga ccaagaagat gtccaagtct gacgataacc gcaataacgt tatcggcctg
    26881 ctggaagatc cgaaatcggt agtgaagaaa atcaaacgcg cggtcactga ctccgacgag
    26941 ccgccggtag ttcgctacga tgtgcagaac aaagcgggcg tttccaacct gctggatatc
    27001 ctttctgcgg taacgggcca gagcatccca gaactggaaa aacagttcga aggcaagatg
    27061 tatggccatc tgaaaggtga agtggctgat gccgtttccg gtatgctgac tgaattgcag
    27121 gaacgctatc accgtttccg caacgatgaa gccttcctgc aacaggtgat gaaagacggc
    27181 gcagaaaaag ccagtgtaca cgcttcccgt acgctgaaag cggtgtacga agcgattggt
    27241 tttgtggcga agccgtaata tcaaaacgct cggacggtcc cctcgcccct ttggggagag
    27301 ggttagggtg aggggagcag gcaagcgcca gtgggaatta ccctttgctc gcattgtcga
    27361 ataaatcact tcgtcatcac tctgaaaccg gggaaacccg gtttttttat cctcatttgc
    27421 aataatccga aaaaatgtga agcgcctcgc cgtttccaca tctcacagtt gccacttatc
    27481 ttcattttaa tgaagatata aacattcatt ttattgaaaa tatattatgc gtcgaacgtt
    27541 tatcaaaaaa gaaggcgtcg tcatcacgac gctggcccgt tatttgttgg gtgaaaaatg
    27601 cggtaatcga ctgaaaacca tagatgagct ggcaactgaa tgccgttcat cggttggcct
    27661 gacgcaggcc gcgttgaaaa cgttggaatc aagtggagcg atacggattg aacgccgtgg
    27721 gcgcaatggc agttatctgg tcgaaatgga taacaaagcg ctgctgagtc atgtggatat
    27781 caataacgtt gtgtgtgcca tgcctttgcc ctatacccgc ttgtacgaag gtttagcaag
    27841 cgggctgaaa gcccagtttg atggcattcc tttttattat gcgcatatgc gtggcgcgga
    27901 tattcgtgtg gagtgtttgc ttaacggtgt ctatgacatg gcagtagttt cccgcctggc
    27961 ggcggaaagt tatctcagcc aaaacaattt gtgcatcgcc ctcgagcttg gcccgcatac
    28021 gtatgttggt gaacatcaac tgatatgccg caaaggtgag tctggaaatg ttaagcgagt
    28081 aggtctcgat agtcgttcgg cggatcagaa aatcatgacc gatgtctttt ttggcgatag
    28141 tgatgtggaa cgtgtcgatc tctcttatca cgagagttta caacgcattg ttaaaggcga
    28201 tgtcgatgcg gttatctgga acgtggtggc ggaaaacgaa ctgaccatgc tgggattaga
    28261 ggcgacgccg ctcacagacg atccgcgatt tttacaggcc accgaagccg tggtcctgac
    28321 gcgagtcgat gattacccaa tgcaacaact gctgcgtgcc gttgtagata aacacgccct
    28381 gctcgcccat caacaacggg tagtgagtgg ggaacaggaa ccgagttatt aaacgaaagg
    28441 catctgatat ggaaaccaga ctcaacctgc tttgcgaggc aggcgttatt gataaggaca
    28501 tctgcaaagg catgatgcag gtcgtcaacg tactggaaac agagtgccat ctgccggtgc
    28561 gcagtgagca aggaacgatg gcgatgacac atatggcgag tgcactgatg cgcagtcgcc
    28621 gtggtgaaga aatagagccg ctggataacg aattgctggc agagctggcg caatccagcc
    28681 actggcaagc cgttgtgcaa ttgcatcagg tgttgttgaa ggaattcgca ctggaagtta
    28741 atccgtgtga agaaggctat ttgctggcca acctttatgg attatggatg gctgctaacg
    28801 aagaggtttg aagctcccga caaatccacg tcgcacaacc taaatattca aaaaaatgaa
    28861 tatttaattc aaggaacaga aacgaggcaa caagatgttt gtagaagcat tgaaacgcca
    28921 gaacccggcg ctgatttccg ccgcactaag tttgtggcag cagggcaaga tcgccccgga
    28981 cagctgggtg atcgacgtgg atcaggtact ggaaaacggt aagcgactga ttgagacggc
    29041 gcggctttac ggcattgaac tgtatctgat gaccaagcag tttggccgta acccatggct
    29101 ggcggaaaaa ttgctggcat taggttacag cggcattgtg gcggtggatt acaaagaggc
    29161 gcgagtcatg cgccgcgctg gtttgtcggt ggcgcatcag gggcatctgg tacaaatccc
    29221 ttgtcatcag gttgctgacg ccgttgaaca gggcaccgac gtcatcaccg tgtttactct
    29281 cgacaaagcg cgggaagttt ctgcggcagc ggtgaaggcc ggacgtgtgc agtttgtgct
    29341 gctcaaagtt tatagcgacg atgattttct ttatccgggg caggagagcg gttttgtaca
    29401 gcattcgctg cacgaggtgg ttgctgaaat taagaaactg ccgggtctgc atttagccgg
    29461 acttacccat ttcccttgcc tgctttggga tgaggctgcc ggaaaagttt tgccgacacc
    29521 gaatcttcac acgctgatac aggcacggga tcaactggcg aaatctggta ttgcacttga
    29581 gcaactgaac gcgccttcag cgaccagctg cacttcgctg ccattactgg cgcaatacgg
    29641 tgtgactcat gccgaacccg gtcatgcgct gacgggcact attccggcaa accagcaggg
    29701 cgatcagtct gaacgtatcg cgatgctctg gttgagtgaa atctcccatc atttccgtgg
    29761 cgacagctac tgctacggcg gcggttacta ccgtcgtggt catgcgcaac atgcgttggt
    29821 gtttacgcca gaaaatcaaa agattactga aaccaatctc aaaactgtgg atgacagcag
    29881 tatcgactac accctgtcgc tggcaggcga gtttccggta agcagtgcag tggtgctctg
    29941 tttccgcacg cagatttttg tcacccgtag cgatgtggtg ctggtgtccg gtattcaccg
    30001 tggcgaaccg aaaatcgttg gtcgttatga cagtcttgga aactctctgg gggcgtaatg
    30061 gcgcgatttg tggtgttagt gattgatagc tttggcgtag gggcaatgaa agatgtcacg
    30121 ctggtgcgtc cgcaagatgc gggagcgaat acatgtggtc acatcctgag ccagttgccg
    30181 catttgcagc taccaacgct ggagaagctg gggctaatca acgcattggg ttatgcgcca
    30241 ggcgatatgc agccgtcaga ttccgcaacc tggggcgtgg cagagctgca acatgaaggt
    30301 ggcgatacct ttatggggca tcaggaaatt ttaggcacgc gcccgttacc gccgctgcgg
    30361 atgccttttt gcgatgtgat tgaccgtgtt gagcaggcat tagtatccgc cggttggcag
    30421 gtggagcgcc gtggcgatga actgcaattt ctgtgggtca atcaggcggt tgcgattggc
    30481 gataatctcg aggcggattt aggccaagtc tataacatta ccgccaatct ctctgtgatc
    30541 tcttttgacg acgcaatcaa aattggtcgt atcgtgcgtg agcaggtaca ggtcggtcgg
    30601 gtcattacat ttggtggcct gttaaccgac agtcaacgca ttctcgatgc cgcagaaagc
    30661 aaagaagggc gctttattgg tatcaatgcg ccgcgttctg gcgcttatga caacggcttc
    30721 caggtcgtgc atatgggcta cggtgtcgat aaaaaagtgc aggtgccgca aaaactgtat
    30781 gaggcgggcg tgccaaccgt gctggtgggt aaggtggcag atatcgtcag caatccttat
    30841 ggcgtgagct ggcaaaacct ggtggatagc cagcggatta tggatatcac cctcgacgaa
    30901 tttaacaccc atccgacggc gtttatttgc accaatatcc aggaaaccga cctcgctggt
    30961 catgcagaag acgtcgcacg ttatgccgaa cgtttgcagg tcgttgaccg taaccttgcc
    31021 cggcttgttg atgcgatgca gccagatgat tgcctggtcg tgatggcgga tcacggcaac
    31081 gatccgacca ttggtcacag ccaccatacc cgcgaagtgg tgccgctgct ggtttatcag
    31141 caaggcctgg ctgctacgca gctcggtgtg cgcaccacgc tttctgatgt gggagctacc
    31201 gtgtgtgaat ttttccgtgc gccaccgcca caaaatggtc gctcttttct ttcctccctc
    31261 cggtttgcag gagacaccct atgagttttg atccgacggg ttacaccctg gcccatgagc
    31321 atctgcatat tgatctctcc ggctttaaaa acaacgtgga ctgccgcctt gatcagtatg
    31381 cgttcatttg ccaggagatg aacgacctga tgacccgggg tgtacgtaat gtgattgaga
    31441 tgaccaaccg ttacatgggg cgcaatgcgc aatttatgct cggtgtaatg cgcgaaaccg
    31501 ggatcaacgt ggtggcctgt actggttatt accaggatgc gtttttcccg gaacatgtgg
    31561 tgacccgcag cgtgcaggaa ctggcgcagg agatggtcga tgaaattgaa cagggtatcg
    31621 atggcacgga gctgaaagcc gggctcatcg cggagatcgg ctccagcgaa ggaaagatta
    31681 cgccgctgga agagaaggta tttattgccg ctgcgctggc gcataaccag accggacgcc
    31741 cgatctccac gcatacgtcg ttcagcacca tggggctgga acaactggct ctgttgcaag
    31801 cccacggagt tgatctttcg cgcgtcaccg ttggtcactg cgatctgaaa gacaacctcg
    31861 acaacatttt gaagatgatc gatctcggcg cgtacgtgca gttcgacacc attggtaaga
    31921 acagttacta cccggacgaa aagcgcattg cgatgctcca cgcgctacgt gaccgtgggt
    31981 tgctgaaccg cgtcatgctg tcgatggata ttacgcgccg ctcccattta aaagccaatg
    32041 gtggttatgg ctatgactat ttattgacca cctttattcc gcaattgcgc cagtcaggat
    32101 tcagtcaggc cgatgtggat gtgatgttac gtgaaactcc ctctcaattt ttccaataag
    32161 gacagactca tgaaaaagat tggcgttgca ggcttacagc gtgagcagat taaaaaaacc
    32221 attgaagcga cggctcctgg cagttttgaa gttttcattc acaacgacat ggaagcggca
    32281 atgaaagtga aatccgggca actggattac tacatcggtg cgtgtaatac cggtgcgggc
    32341 gcggcattgt cgattgccat cgcggtgatt ggctataaca aaagttgcac cattgccaaa
    32401 ccaggcatta aagcgaaaga cgagcatatc gccaaaatga tcgctgaagg aaaagtggcg
    32461 tttggccttt ccgttgagca cgtcgaacac gcgattccga tgctgattaa ccatctgaaa
    32521 taaaaggcac gactatggat ctgtatattc agattatcgt ggtggcgtgc ctgacgggta
    32581 tgacatcgct tctggcgcat cgctcggcgg ctgtttttca tgacggcatc cgcccgatcc
    32641 tgccgcaact gattgaaggc tatatgaacc gtcgcgaggc ggggagtatc gcttttggtc
    32701 tgagcattgg ttttgtggcc tcggtgggga tctcttttac cctgaaaacc gggctgctca
    32761 acgcatggtt actctttctt cctaccgata tcctcggcgt actggcgata aacagcctga
    32821 tggcgtttgg tcttggcgct atctggggcg tgttgatcct tacttgcctg ttgccagtaa
    32881 accagctgct gaccgcgctg ccggtggatg tattaggtag cctcggggaa ttaagctcgc
    32941 cggtagtttc tgcttttgca ctgttcccgc tggtggcgat tttctaccag tttggctgga
    33001 agcaaagtct ggtcgccgcc gtggttgttc tgatgacccg tgtggtagtc gtgcgctatt
    33061 tcccacatct taaccctgaa tccatcgaaa tctttattgg catggtgatg ctgctgggga
    33121 tcgcgataac tcacgacctg cgtcatcgtg atgaaaatga tattgatgcc agcgggcttt
    33181 cggtgtttga agaacgcacg tcgcggatta tcaaaaactt accctatatc gccatcgtgg
    33241 gagcattgat tgccgccgtt gccagcatga agattttcgc cggcagtgaa gtgtcgatct
    33301 tcactctgga gaaagcgtac tccgcaggcg taacgccgga acaatcgcaa acgctgatca
    33361 accaggcggc actggcagaa tttatgcgcg ggctgggttt tgtaccgttg attgccacca
    33421 ccgccttagc aacgggtgtg tatgcagttg cgggctttac ctttgtttat gcggtgggct
    33481 atctctcgcc gaatccgatg gttgcagcgg tattaggcgc agtggttatt tcggcggaag
    33541 tcttgctgct tcgttctatc ggcaaatggc tgggacgcta cccgtcggtg cgtaatgcgt
    33601 cggataacat ccgtaacgcc atgaatatgc tgatggaagt ggcgctgctg gtcggttcga
    33661 ttttcgcagc aattaagatg gcgggttata ccggattctc tatcgcggtt gccatttact
    33721 tcctcaacga atccctgggc cgtccggtac agaaaatggc ggcaccggtc gtggcagtaa
    33781 tgatcaccgg tattctgctg aatgttcttt actggcttgg cctgttcgtt ccggcttaag
    33841 gaaggcacct atgaagacgt ttcctctgca aagcctgacg cttattgagg cgcagcaaaa
    33901 gcagtttgcg ctggtggaca cgatttgccg tcactttccc ggcgcggagt ttctaaccag
    33961 cggtgatttg ggcttaacgc cggggctgaa tcaaccgcgt attacccaac gggtggagca
    34021 ggtgctggct gatgcatttc acgcacaggc tgcggcgctg gtgcagggcg cggggactgg
    34081 cgcgattcgc gccgggctgg cagctttgct caaaccgggg cagcgtcttc tggtgcatga
    34141 cgcgcctgtt tacccgacga cacgggttat tattgagcag atggggctga cgcttattac
    34201 tgttgatttc aatgacctgt cggcactgaa gcaggtcgtc gacgagcaac aaccggatgc
    34261 ggcgctggtg cagcatacgc gccagcagcc gcaggacagc tacgtgctgg cagatgtgct
    34321 ggcaacgttg cgcgcggcag gtgttccagc gttaaccgat gacaactatg cggtgatgaa
    34381 ggtggcgcga atcggctgtg aatgcggcgc gaatgtctcg acattttcct gcttcaagct
    34441 atttgggcca gagggtgttg gtgcagtggt cggcgatgct gatgttatca accgtattcg
    34501 cgccacgctt tactccggcg gtagccagat ccagggcgca caggcgctgg aagtattgcg
    34561 ttgtctggtg tttgcgccag tgatgcacgc ggtgcaggca ggggtatctg aacggttgct
    34621 ggctttgctt aacggtggtg aggtgccgga agtgaaaagc gcggtgattg ctaatgcgca
    34681 gtcgaaggtg ttgattgtcg agtttcatca gccgattgcc gccagagtgc tggaagaggc
    34741 gcaaaagcgc ggtgccttgc cttacccggt gggtgcagag tcgaaatatg aaatcccgcc
    34801 gctcttttat cgcctttccg gaacgtttcg ccaggcgaat ccacaatcag aacattgtgc
    34861 gattcgcatt aacccgaatc gcagcggtga agagacggtg ctgcggattt tgcgtgagag
    34921 tattgccagt atttaatgct ggatagcacc gtcaagctaa attccgtact gaacggtccc
    34981 ctcgcccctt tggggagagg gttagggtga ggggaacgta cgggatgtaa aattcaacgc
    35041 gccagcatcg ccggatgcga tgctggcgca tcttatccgg cctacggtta atgtctttta
    35101 ttatcaatag ttaaggtgat gcgattggcg cggcagaaga gttcggagac gtgcaccggt
    35161 ttgtcattct gatcaaaggc gattttgctg atgcgaaaca acggttcgcc cagttcgcat
    35221 ttcagccatt ttgcctgcgg gcgggtggcg gcaaagatat cgatggtctt tttgtcgctg
    35281 accactcgcg tgtcaaaacg ctcctgaaat aactgatagg tggagcttcc ttcgacgtaa
    35341 atctcatcaa agtcaggata acgcgacagc gggatccatg aactatcaat aaacagcggc
    35401 tctttatcga gatacatcac ccggcagaga tggaacactt cgctgtttcc ggggatgttc
    35461 agctttttgc aaaacggcgc ggcgctgacc cgttcctgtt cgatcacttt ctctttcgtc
    35521 gccttgcctt gtgagacgcc aaaatcggta aaaccactga cagtaagcag ggcgttttca
    35581 actttctggc tttgtacaaa ggtgcctttt ccctgccagc ggatcagtac gccgtccgcc
    35641 actaagtcgc tgatggcttt gcgaatggta atgcggctga cgttatattg tgtacaaagc
    35701 tcgttttcgg tagggatctg ttgcccggcc tggtaaaccc cctgcgcgat atcatccagc
    35761 agtcgctggc ggacggtagc gtagaggagt tgatgagagt agcggtccgt agctgacata
    35821 atcgaccctg aaataaagaa tgaattactc ggattatagg gtcgttttcc ggcaagtaac
    35881 agcacgttgg cgtgaagatg ctcacgccaa cgttatacct accaggcacc gtggtactga
    35941 atggttttcg ccgcgcacgc cgttccctgc gccatcgcct gcggtaatgt catccccgca
    36001 gaccagccgc aaaggaatcc ggcaatgaac gaatctccgg cacccatggt gtcgataacc
    36061 gtcaccggtt caggagcctg acgccagaac tgcgcgccat cccaggcaat gctgccgttt
    36121 tcacccagcg tgacaatcac tgttcctgcg ccacgggcaa caatcgcttt catcttcaga
    36181 cgcagcgttt cgtcttcttg cggtgcggag gcaaaggcaa aatcgagatg cggcaccagt
    36241 gtctgccaga gcgggctgtc ccacttgtcg gagaagtcga aagcggtaag tttgcccgca
    36301 gcgtgcagct gtgggaatgc gtcttccgca tgtccccaga ttgccgcgtg cacaatgtca
    36361 tactgcgcca gccaggcgta atcctcttca ctcagggcaa agtcggccat cacgccttcg
    36421 gtgtagtcgc caaaaacgcg atcattgtcg tgcagttcca cctgagtttg tgcggtaacg
    36481 ccgtgtttcg tatggacatg gctgatatcg acgcccatgc gggcgagatc ctgcttcagc
    36541 tttgtgccgt agtcatcgtc acccacccag gtaatgcatc ccggctgtat gccgtagcga
    36601 gtgcagtaca ccgccacatt gaccgcatta ccgccagaaa acgctttatt cagttgcggg
    36661 tagatatcga cgcagttatc gccgattgtc gccagggttt tcatctctca tcctccggca
    36721 gcagcgcgcg aaagcgttcc agcgcctggc gggcatagag tctgggctcg ttcatataca
    36781 tcgtcaccag ctccaccgta cagtaaccct catagccccg ctcaataata tcgcgcatca
    36841 gttcccgcag cggcattttg ccttctccag gaatgtaatg cgtgtcgctg gccccgtcgc
    36901 tgtcgacaat atgcagatga cgtaatttat cgcccagttt gtcgaaataa ctcatcaccg
    36961 gttccgcctg gacatacggc gcgcaaatgt cgaccatgct gaacaggcgc ggcgaaggca
    37021 ccagcgccag cgcatgaagc acatcattag cattacacac gatgttcgat tcatacggcg
    37081 ttaacggttc gaggattaga tccatgccga tgttttccgc gtactcatac agttcgctta
    37141 ggttctctgc cagccgtccc cagataacat taggtggcgt gagatagccc gcgtgggccg
    37201 cggaaatcag cgtataaccc gcgttcatct cttttgccat atccatcgcc agcttgatca
    37261 tgtcgaggct ttcgcgacgc atatgttcat cgcccagcat catgttatac ggatagccat
    37321 tggtttctgg cgtatagccg ataatcggca tctgatacgt ctgcgccagc gccttgattt
    37381 gtttgatgcc gcccgctttt aagtccggcg cgaacgcgtg cgggcgaccg ccccaaattt
    37441 cgatgccgtc gtaacccagc tcgcttgcat cacgaaatgc gtgttcaata ggcagccgct
    37501 ggtggccgca ggtaaacata cctgttttta tcgggtaact ccttgcgtaa atccggcccg
    37561 ttgccgggcc gggcgaggga ttaatattcc accagaccac cgtaatagcg gcgttcatcc
    37621 gggttgtgat ctttgtaaat agacaggtag tagcagagcc actccattgg cacgaacatc
    37681 aggaacggtg ccagccacgg gtgcagccct tgcgaaattt cggcgtaatc gatgacgatc
    37741 acgttgtcag tacgctgttt aacaaagtta atggcgcgtt cggtggtgtg gcgactttca
    37801 tcattgccga gcaggaacag gaacggaacg cccggttcga caatctccag cgggccatgg
    37861 cggaactctc cgctctcaat cacgcagccg tgcgtccagg taaattccat cagcgttaca
    37921 atgccttctt tgtaacccag cggacgcagc ggacccgcag caacggtata aatcatcggc
    37981 cactggctgg ccagttcacc aagctggcgg cctttttctt cccaggtgcg taccagatga
    38041 ccgagcgcat tcggcaactg cttgagatcg tttttgatct tgccgatttc cgcgttcggc
    38101 gcgaggcggg tgatcatctc cagcaccacg ctgtagcaga gcagcaggtg aatttcccag
    38161 atacagtcgg cctgataatc aatgctaaat tccgccgccg aggtaatcgg gctgtccgcg
    38221 cgtttggtga acgccgcagt gagtgcgccg caggcccggc ccagctccag cgctttgatt
    38281 acctcttccg ttttaccgta gtcagaaacg ccaatcaccg cgcaacgatc gtcgaggcga
    38341 tacggggtgt tatcgcagaa ctcccagccg gaaatggcgt agacctgaag atcggaaaag
    38401 cgatccgcca gatgtttcgc cgcttgcgcc gcgttgagtg gcgagccgca ggcaacgaaa
    38461 taaatacgat caatgtcgcg cttcaccatc tcttccacga tggcgtgcac cagcggaacg
    38521 tcatggctga gaactttttc cacttcctga accatatttt cggtgaccag aaagtccacg
    38581 gtgcttttat caatatccaa cattttattt tctccagata acattacgac aaagcgttga
    38641 gctggcggct acgcttcgcc cagaaggcgt aagcaggcag accagtagca ataacgatga
    38701 cggcgcagat aaggccggga atcggtgccc agacaaaggt tgaggcgacc agaatgaggc
    38761 ttgacgcaat ggcgagggtg gtcatcagcc cgaaagcagg agtacgccac agcggtttgt
    38821 aatcgtcgcg tttacgacac cagatgatgg agccgaaggt gagggtattt ttgaaacaca
    38881 tcaccagggt gaaataaccc agcaggctgg tgagatcgga aacgaagatg aagaagagcc
    38941 ccagcgcccc ttgcaggatg atggagacat ccggcgtgtt gtatttcgga tgcacatggc
    39001 cgaagcattt aaagaacagg ttgtctttcg ccatcgcgta ttccagccgc ggctggtaca
    39061 tcacgcagct ggaaagcgaa ccaagaatga cgatcatcgc cgtgatggca acaaagatcc
    39121 cagcggtgct gccgagtgcg gggatccagg tcagggcgtc ggaaatcggc gtttcagaat
    39181 tggcgagttt gtcgaagggc atcaggtcgg aaatcaccag cgccagcagg gtgtagagca
    39241 ccagaaccag cagacaggaa ccaatcagcg ctcgtggcat ggtttttccg gggtttttaa
    39301 tttcgccggt catataacag atagaggcca tgccggtata cgaccaactg gtggcagaga
    39361 tccccgccag cagcgccata aagctgcccg ttgcgccaat cgcagtggtg gtaggggcgg
    39421 caaaattctc cgctttaaac cagaagatcc caaggccaat gacgatagtg aacgggataa
    39481 ttttggcgat ggtaattagc gtctgaaacg ctgcgccgcc ttcaacggag cgcaggtgta
    39541 gcaacataaa ggcgataatt aatccggcgg cgataaattt accgggcaac ggatcgatag
    39601 gcgttaaaaa gccaagattg ctgacaatcg ccagcgccat aatcgacaat gacggcgcat
    39661 cgttggccca gaagctggcc cagccggaga ggaaagccag cggtcggctt ccggcatttt
    39721 tcagataaac ataatctgcg ccattttccg gataagcggt ggatagttcc gcatagacgc
    39781 acatttgcgg gatcacaatt aacccgccaa tgacaaacgt gagcaccgta agccacggcg
    39841 tgcccgctgc ttttgccact tcacccacag atacaaaaat accggagccg acggttgtcc
    39901 cgacggcgat tgcaagaacg gcccaaaatc cgagcttgcg ttggagttcc tggcttccca
    39961 tagcgatacc tttttcttgt ccaacacgtt gtaggggaga gtttgcagtc atttaaaagg
    40021 taacatgaca atgcatgatg aatataataa catatgatgt tatgtaatgc ggaggaagat
    40081 cacaaaattt cgcacaggat cgcgctgtgg ctaatgaatg tagttatcca attgaaatta
    40141 aagtgaaaat atttttacgg gtggggagca agaaggatat agaaacaaat acgccctcgg
    40201 aaaatccaga gggcgtcggg caattaaacc ggtgttagcc gatttctgtc agagacttac
    40261 tgtgcagtag gaccgcaacc gccaatgatt ttagaaatag aaattgccgg gtgcagcagg
    40321 tagtcgtaag agcagttctt atcacggttt tcaatatgac cagtacatgc agttaacgta
    40381 gccagtactg cacctaccag tgcaacttta ataaatttgt tcatttac
//
LOCUS       DAJTMC010000014        40058 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.014, whole genome shotgun sequence.
ACCESSION   DAJTMC010000014 DAJTMC010000000
VERSION     DAJTMC010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40058
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.014"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(457..2148)
                     /gene="fruA"
                     /locus_tag="OO130_000668"
     CDS             complement(457..2148)
                     /gene="fruA"
                     /locus_tag="OO130_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HCR5364187.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAVARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVAATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            complement(2165..3103)
                     /gene="fruK"
                     /locus_tag="OO130_000669"
     CDS             complement(2165..3103)
                     /gene="fruK"
                     /locus_tag="OO130_000669"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HCR5364188.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            complement(3103..4233)
                     /gene="fruB"
                     /locus_tag="OO130_000670"
     CDS             complement(3103..4233)
                     /gene="fruB"
                     /locus_tag="OO130_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HCR5364189.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEAGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFAVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            4601..5782
                     /gene="setB"
                     /locus_tag="OO130_000671"
     CDS             4601..5782
                     /gene="setB"
                     /locus_tag="OO130_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HCR5364190.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPAILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRTKD
                     V"
     gene            complement(5779..6033)
                     /locus_tag="OO130_000672"
     CDS             complement(5779..6033)
                     /locus_tag="OO130_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HCR5364191.1"
                     /translation="MECRPGCGACCTAPSIFSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAS"
     gene            6188..6760
                     /gene="yeiP"
                     /locus_tag="OO130_000673"
     CDS             6188..6760
                     /gene="yeiP"
                     /locus_tag="OO130_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HCR5364192.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            6983..8449
                     /locus_tag="OO130_000674"
     CDS             6983..8449
                     /locus_tag="OO130_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCR5364193.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLGMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFVAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAHISDCMQDRAFRHAARTLMLNE
                     QAPTLQIKDVDLTQYADKLIARFANPALKHKTWQIAMNGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            8567..9553
                     /gene="yeiR"
                     /locus_tag="OO130_000675"
     CDS             8567..9553
                     /gene="yeiR"
                     /locus_tag="OO130_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HCR5364194.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWTVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            9592..10305
                     /gene="lpxT"
                     /locus_tag="OO130_000676"
     CDS             9592..10305
                     /gene="lpxT"
                     /locus_tag="OO130_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HCR5364195.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPVNHGLWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIIGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTNINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            10717..11283
                     /gene="mepS"
                     /locus_tag="OO130_000677"
     CDS             10717..11283
                     /gene="mepS"
                     /locus_tag="OO130_000677"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HCR5364196.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            11464..13020
                     /locus_tag="OO130_000678"
     CDS             11464..13020
                     /locus_tag="OO130_000678"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HCR5364197.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGGETPEQARWLSEPGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            13102..14916
                     /locus_tag="OO130_000679"
     CDS             13102..14916
                     /locus_tag="OO130_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR5364198.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYRVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADTLLNEAGWVLKGQQRVNATTGQQLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSHPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            14917..16011
                     /locus_tag="OO130_000680"
     CDS             14917..16011
                     /locus_tag="OO130_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HCR5364199.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     HNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTILTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            16011..17036
                     /locus_tag="OO130_000681"
     CDS             16011..17036
                     /locus_tag="OO130_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5364200.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            17038..18627
                     /gene="yejF"
                     /locus_tag="OO130_000682"
     CDS             17038..18627
                     /gene="yejF"
                     /locus_tag="OO130_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HCR5364201.1"
                     /translation="MTQTLLAIEDLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFRGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNYAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LINSQGSIIFDGQPLQNLNRRQLLPIRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKLQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFAAPQQEYTRQLLALS"
     gene            complement(18631..18975)
                     /locus_tag="OO130_000683"
     CDS             complement(18631..18975)
                     /locus_tag="OO130_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5364202.1"
                     /translation="MTSLQLSIVHRLPQNFRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(19308..20491)
                     /gene="bcr"
                     /locus_tag="OO130_000684"
                     /pseudo
     CDS             complement(19308..20491)
                     /gene="bcr"
                     /locus_tag="OO130_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311101.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
     gene            complement(20519..21214)
                     /gene="rsuA"
                     /locus_tag="OO130_000685"
     CDS             complement(20519..21214)
                     /gene="rsuA"
                     /locus_tag="OO130_000685"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HCR5364203.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            21363..23123
                     /locus_tag="OO130_000686"
     CDS             21363..23123
                     /locus_tag="OO130_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCR5364204.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            23248..23532
                     /gene="rplY"
                     /locus_tag="OO130_000687"
     CDS             23248..23532
                     /gene="rplY"
                     /locus_tag="OO130_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HCR5364205.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(23671..24678)
                     /gene="yejK"
                     /locus_tag="OO130_000688"
     CDS             complement(23671..24678)
                     /gene="yejK"
                     /locus_tag="OO130_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HCR5364206.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTKFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            24860..25087
                     /locus_tag="OO130_000689"
     CDS             24860..25087
                     /locus_tag="OO130_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HCR5364207.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            25107..26867
                     /gene="pbgA"
                     /locus_tag="OO130_000690"
     CDS             25107..26867
                     /gene="pbgA"
                     /locus_tag="OO130_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HCR5364208.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRPQSDEQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALLDSGKLDNTVVIITAG
                     RGIPLSEDEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            26942..27018
                     /locus_tag="OO130_000691"
     tRNA            26942..27018
                     /locus_tag="OO130_000691"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26976..26978,aa:Pro,seq:ggg)
     gene            27237..28478
                     /locus_tag="OO130_000692"
     CDS             27237..28478
                     /locus_tag="OO130_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000101915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5364209.1"
                     /translation="MARKTPPLTTVQIKAARPAEKEYTLQDGGGLFLLVKPSGSKLWR
                     FSYYRPSDKKRILLSFGSLDDVSLADARKRRSEYRALISAGTDPQGHEKKKREAEARR
                     QGNTFENVAAAWYQVKISQNLAPNTIKDIWRSLDKYVFPFIGNTPIDTLTARRFVEVL
                     TPIKERGNLETLKRVLQRVNEVMDYAANSGLIDANPAMNVRKAFPSPVKKHMPTIRPE
                     QLPELMQALSVSATERQTRLLIEWQLLTVTRPAEASSTRWDEINLDAKQWTIPAGRMK
                     MRRDHVIPLSGQAMAVLEAMKPISHHRNYVFPSLKDPQQPMNSQTANAALRRMGFAGV
                     LVSHGLRAIFSTAANEEGFEPDVIEAALAHVDTNEVRRAYNRSNYIEKRIVLMRWWGE
                     FVEAAATGVTLASGKRGIRAV"
     gene            28974..29180
                     /locus_tag="OO130_000693"
     CDS             28974..29180
                     /locus_tag="OO130_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
                     /protein_id="HCR5364210.1"
                     /translation="MEAIRKIIFRQEVKKIIHIKADSTLQSMINAGEFPQGFRVGLRR
                     RGWYEDDVLAWQKEREQEARGTAA"
     gene            29195..29860
                     /locus_tag="OO130_000694"
     CDS             29195..29860
                     /locus_tag="OO130_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001329530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5364211.1"
                     /translation="MTNTKKAAPIWSRLSEQLTRCALCVYDPKHKHGDDSRYQAGGQC
                     NQSGSVRCHTCNERFSLYSLRNCSRAKAHGANLSDSCSIFLRRLFRAGDNVLVNSLSV
                     IVLSCIAMRRNTSHHGADSFYPLGSMPRRFSSVRPNSLSQLARLMPSLCASLSNCSFS
                     SGEIRILNCGDCPSPLGLLSRLIIDKWSPIELAFPLLGGHLNTRALKKAKPRKCHYHS
                     QGF"
     gene            29886..30086
                     /locus_tag="OO130_000695"
     CDS             29886..30086
                     /locus_tag="OO130_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HCR5364212.1"
                     /translation="MVAVNHIPHLVHTQTAFVWRFLALSAGESQIIHVTAWTEREARS
                     RCPSGCVAVFAARIRQGETYAQ"
     gene            30076..30297
                     /locus_tag="OO130_000696"
     CDS             30076..30297
                     /locus_tag="OO130_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5364213.1"
                     /translation="MHNKTTPNAAAAALTTLMHALIDIECTAELAQGEEQKDRTQFAL
                     ECIRYIATRSLNDAKNILVADCENGGGYA"
     gene            30290..30523
                     /locus_tag="OO130_000697"
     CDS             30290..30523
                     /locus_tag="OO130_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5364214.1"
                     /translation="MRDDRFNSLKQEFPGVPDDAADALSSMPELIRAAFFLLSTREYK
                     STGLDVLNIAADYAEYVAEARYRRKFPEDVSHA"
     gene            30516..30749
                     /locus_tag="OO130_000698"
     CDS             30516..30749
                     /locus_tag="OO130_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5364215.1"
                     /translation="MRDIYHETIDRAFLALSHSENMLEILRIWLETLGDNERDKQKSR
                     IATALITLLEPVIMELQEIDLLHDRYKEQHTGE"
     gene            30755..31054
                     /locus_tag="OO130_000699"
     CDS             30755..31054
                     /locus_tag="OO130_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HCR5364216.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGTVMIKGVEERRVTY
                     RREGYEYDCVMPVYQFRRDFSLVQTAPHNVPTSNARARANIQKLKTMINGFRGKK"
     gene            31051..32451
                     /locus_tag="OO130_000700"
     CDS             31051..32451
                     /locus_tag="OO130_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR5364217.1"
                     /translation="MKSAPNLKKQPYDKMTEVIIFAGSDAWAHAKQWQEQDGRLAGDN
                     VPPVVLADDQLDELADLRIIDEGRYCVRLYKAGHIRPSNINAIAHKLAAAGVTDANYY
                     PEGMHSHMRENWREYLERVRGKEPVEEKNHQRKTTLPMSVGSTGYDTQLDYVVKGIIP
                     AVSLCSIYGASGSYKSFLAGSWACHVATGRQWGGRRVAHGAVLYVVGEGGIGVPRRVK
                     AWEVVHDEQVKNLYLVNRPIFPAAPLDVDEMVIAARQVERETGKPVRMIILDTLARCF
                     GGNDENDSRDMGAFIRGCDELKRRTGATVLVVHHSGKDETKGARGSSAFRASLDAEYR
                     IRREDAGSEALVISCTKMKDAEELKEAAYDLRVVELFTDADGELITSLVVVDDPRPPV
                     ELERIEEAGNKTENHTALWGCIRSRTQNGDKCTIPLLRDDMKKLGYEMKNFRRWLYKL
                     EKDGVISIDGDDVAPL"
     gene            32651..32902
                     /locus_tag="OO130_000701"
     CDS             32651..32902
                     /locus_tag="OO130_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5364218.1"
                     /translation="MTQKRRDRTEPKYKALDMTEHTLKVAIRTIDRHTREGYAKEHPD
                     LISAFMTTAAANFATLTEREIAEAEQVTTINVKTGEVEL"
     gene            32899..33309
                     /locus_tag="OO130_000702"
     CDS             32899..33309
                     /locus_tag="OO130_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCR5364219.1"
                     /translation="MTAQIAAYGRLVDDPQVKQTSKGTPMTLARMAVSLPCSQAQDGQ
                     ATLWLSVIAFGKQADFLAKHQKGDVASVSGTMQVSQWTGQNGETRQGWQVIADSVISA
                     RAARPGGNRRKTTGTQGNQPPAGGDDPYGDDISF"
     gene            33320..33592
                     /locus_tag="OO130_000703"
     CDS             33320..33592
                     /locus_tag="OO130_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HCR5364220.1"
                     /translation="MVHDRIAEELEAKGFYRRAAARWGEVMQLVETDKERHHITMRRL
                     ECSRKAQRAPEPPDNFGDLKKAVDRTYAEMGIDGAGDEIWRNYQDS"
     gene            33831..34037
                     /locus_tag="OO130_000704"
     CDS             33831..34037
                     /locus_tag="OO130_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000796968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5364221.1"
                     /translation="MAEEKKGGVSVYISPEIVEVLKQRHKKNYEAGVAAGLDPLMTPE
                     PSIGSLVRSYLLAALGMHKNYGGE"
     gene            34037..35092
                     /locus_tag="OO130_000705"
     CDS             34037..35092
                     /locus_tag="OO130_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HCR5364222.1"
                     /translation="MAGKATALNTNQLFMYLNRGDIADFKFSPLFTTLFFPNVATFST
                     QNIMLDTLDIEEVTMSAFCSPMVGSQVQRDKGYETSTIKPGYMKPKHEIDPTKTIMRM
                     AGEDPAQLNDPTYRRMRLITGNMRRQINAIKARVEWLAVNAVTTGKNIIEGEGIERYE
                     IDWKIPEKNIIEQSDGKKWSEHDKDIYDPIYDIELYADQAGCPANVMIMGAEVWRTLR
                     SFKKFRELYDLSRGSESAAEMACKNLGEVVSFKGYLGDIALIVYSGKYTDSDGTEKYF
                     LEPDLLVLGNTNNKGLVAYGAIMDQEAVRTGATQNMYYPKNWIEDGDPAIEYVQTHSA
                     PQPVPADIRKFVTVKIG"
     gene            35104..35439
                     /locus_tag="OO130_000706"
     CDS             35104..35439
                     /locus_tag="OO130_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HCR5364223.1"
                     /translation="MDTPYIELFAGSQQVSTTLVHFAADAGVIQEFTPLMLVDNGEFK
                     PWDGQESGKAVYLTSYLVDTSKQKSAQCYKTGIFNIAAVNWPESVDTDAKKCAAFAGS
                     GVSVQPLAR"
     gene            35452..35865
                     /locus_tag="OO130_000707"
     CDS             35452..35865
                     /locus_tag="OO130_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016240519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HCR5364224.1"
                     /translation="MATNESIMALPLASKFKAEARAMADRGLSTYEAVYQLNKLEEQD
                     KPRADAIMALHEHKDYQPLLRAMANVPCIDVDTAKNILNMTIEQERPKVAPELTAAFE
                     NFMDMHSPQTVSAGMAYDGRNPGDDGDIDRILKTI"
     gene            36027..36461
                     /locus_tag="OO130_000708"
     CDS             36027..36461
                     /locus_tag="OO130_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5364225.1"
                     /translation="MIDKACFVSQQEIAEHFKVNRTTIRAWTKQGMPYLNADRGKSGG
                     YHIGHTLLWSSGKSRLEAIRYHVETSALEKIMFARLLSSERDEYSSEETEHRFDEGLQ
                     IYGYSPEDVSKARNKMAGFLAGWRHAVSVRRASMEQSADTEQ"
     gene            36742..37023
                     /locus_tag="OO130_000709"
     CDS             36742..37023
                     /locus_tag="OO130_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5364226.1"
                     /translation="MTEAEILGLIRRAGGISQQTDEQATQPDSVTAENYARVVAEVMR
                     RDGIQLNDVDMRNIRIRVLEMLAYNRRVALYRETEKITYHWKKPERLRR"
     gene            complement(37702..>40058)
                     /gene="yejO"
                     /locus_tag="OO130_000710"
     CDS             complement(37702..>40058)
                     /gene="yejO"
                     /locus_tag="OO130_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HCR5364227.1"
                     /translation="GGYQYIEMNGTAEYSVLNDGYQIVQMGGAANQTTLNNGVLQVYG
                     AANDPTIKGGRLIVEKDGITVLAAIEKGGLLEVKEGGLAIAVDQKAGGAIKASTRVME
                     AFGTNRLGQFEIKNGIANNMLLENGGSLRVEENDFAYNTTVDSGGLLEVMDGGTATGV
                     DKKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYELDDGSGLIVMEDTQAIDTILD
                     EHATMQSLGKDTGTKVQANAVYDLGRSDQNGSITYSSKAISENMVINNGRANVWAGTM
                     VNVSVRGNDGILEVMKPQINYAPAMLVGKVVVSEGASFRTHGAVDTSKADVSLENSVW
                     AIIADITTTNQNTLLNLANLAMSDANVIMMDEPVTRSSVTASAENFITLTTNTLSGNG
                     NFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASPAAGDSLTLVTTGGGDAAFTLG
                     NAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTTDVLNMAAAQPLVFDAELDTVR
                     ERLGSVKSVSYDTAMWSSAINTRNNVTTDAGAGFEQTLTGLTLGIDSRFSREESSTIR
                     GLFFGYSHSDIGFDRGGKGNIDSYTLGAYAGWEHQNGAYVDGVVKVDRFANTIHGKMS
                     NGATAFGDYNSNGAGAHVESGFRWVDGLWSVRPYLAFTGFTTDGQDYTLSNGMRADVG
                     NTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEYADSNHVKVNDDGKFNNDVAGT
                     PGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQAGVVWTF"
BASE COUNT        10079 a        10188 c        10020 g         9771 t
ORIGIN      
        1 agcctataat tacgacataa tccttttcgc gcatagcaca tcctctggta aaaaattacc
       61 aaccctgtca atccacaaga atatagatac tgcaaagacg gtttaaacat gtgttcatat
      121 ttaaacatgt gctcatagca tgcatgtttt acgtaaagta aattaccgcc agagaggcaa
      181 aatgggcaga tttgagcagg ctcacaaaat gcgattaaat gcctgaatta caaaagcgag
      241 gaatttgtga cgggatgcac agaacgggaa caataaaaaa ggcagaaaat aatctgccct
      301 tgaggaaata accttagtgt ttgatgaacg tttcaagttt ctactgaaag ataaaaccat
      361 ttaaattcat aaaattacat tcacaaaccg gtcattctct ttgcgctcat caaatgttac
      421 agggcaggaa atttctgccc tgtaacacac cttttattac gctgctttcg ctactgcgtc
      481 cacttccgga cgtttcagga aggcataggc caaacccgcc accagcgtac cggcaataat
      541 tgctaccagg taacccagta ccggcgtaat agcgccaggg atcagcagaa caaacagacc
      601 accgtgcggt gccatcagtt tcgcaccaat cgccattgag attgcgccag tcagcgcccc
      661 acccacgata cagcacggca gcacacgcat cggatcacga gcagcaaacg gaattgcacc
      721 ttccgaaatg aagcacagtc ccaataccag agcggctttg ccaccttcct gctgcgcttt
      781 gtcgaatttg cgacgcgcca ccattgttgc cagacccatt gccagcggtg gcaccatacc
      841 tgccgccata atcgccgcca tcgggccata ggtttgagta ctcagcagac ccacaccgaa
      901 tgcgtacgct gctttgttta ccggaccgcc catgtcagta cacatcatgc caccgaggat
      961 cgcccccagc agaaccgcat tcgcagtccc catggtctgc agccagtgag tcagcccttc
     1021 gagaatgcca gcaactggtt taccgatcag gtagatcatc gccagaccga ccaccagact
     1081 ggaaattagc ggaatgatca ggatcggttt cagcgcctcc atactctgtg gcagtttcag
     1141 ttgcgtactg attaacttcg caatgtaacc agccaggaag ccagcaatta taccgccaat
     1201 aaagccagaa ccggtgctga ccgccagcat accgccaatc agacccggcg tgaggcccgg
     1261 acgatcggca atggagaagg caatataacc tgccagtacc ggcaccatca gcgcaaaggc
     1321 tgaaccaccg ccaatctgca tcagcgccgc tgccaacgta cccggctctt taaacgcttc
     1381 gataccaaaa gcaaaagaaa gcgcgataca cagaccacct gcgacgacca tcggcagcat
     1441 ataagagacg cctgtcagca agtgacggta agcgcctgca ctctctttct taccttcagt
     1501 ggtcgccgtt tgagctttgc ccgccggttc atacggcgtt gcttcagcaa ccgctttatc
     1561 cagttcctgc gcggttttct tcagcgccag accggtagag gtacgataca tcggtttacc
     1621 agcaaatttc gccagatcca cttcgatatc tgccgccaca atcaccagat ccgctgctgc
     1681 gacttcttca ggggtgattg cattgcccgc gccaacagaa ccacgggttt caactttcac
     1741 ccaccagcca cgttttttcg cttcggtttc aatggcttca gccgccataa aggtgtgtgc
     1801 tacgccagtc gggcaagcag tcaccgcaac tacgcgtttc ggaccgctgg cagcaaccgg
     1861 tgctgtcgca gcgaccggcg cagtgtaagg tttcgcatgg cctttggctt cactcaggaa
     1921 cagctcaggg tgcgcaactg cccgggaaat atcacccagc cagacatttt taccgttcag
     1981 cgcgctgtca ttcgggatgg aatcaccgag aacaatcgcc atttctgcgt cgttcggatt
     2041 gtcgatgatt tccagttttg cttttcgcgc caccgcgccc agcagggtct tcgccatata
     2101 ggcgcgtgcc tgaccgagat tagcgtcaat aatcagcagc gttttcatta tgcctctcct
     2161 gctgtcagtt aaaaggttgt aagtcgacgc gcgccatcat tgcggccaac tgcggacgat
     2221 cggtaatacc cacattgctt tgacttaccg ccagggctgc aacagctgtc gccagacgca
     2281 gtgtgtgttc actggattca cgcatcagca agccataaat caggccacca accatagaat
     2341 cccctgcgcc aacggtgctt acgacatcga ctgacggtgg tttggcgatc cattcgccgg
     2401 aggcattaac ccaaagcgcg ccttcggcac ccagtgaaat aacaacatgc gcaatacctt
     2461 gttcacgtag cgcatgtgca gcttcaatca catctttcat ttcaggcagt ttacggcctg
     2521 cccaaatttc caactcgcgg cggttaggtt tcaccagcca cggtgccgct ttcaaacctg
     2581 ctactaacgc ttcacggcta ctatcaaaga taatgcaagg acactgacta cgcaggcgag
     2641 tcatccagtc ggtgaacgct tccgggctga cgcctgacgg taagcttccg ctgacacaga
     2701 ccatatcgaa ctgaccgagc cagctcagag aatcagtcac aaagcgttcc cagtcggcgg
     2761 gggtgacttc aaaacccgag aagttgaagt cggtcacttc gccgtctttt tccgtcagct
     2821 taacgttaat tcgggtgcgc ccctgtacaa cctggaaacg gttggcaatg cccagctcgc
     2881 tgaacagttg ctgaaaacca tcctgattgt ctttacccag gaagccgcca acggtgacat
     2941 caattcccag gtcttttaat actttggcca cgttgatgcc tttacccgcc gcatgcagac
     3001 cggtggtttt caccaggttc acttcgccgc gttcaatttc cgggcagaaa ccaacaaggt
     3061 cataagccgg attaagggtg atcgtagcaa cacgtctgct cattatgcgc cctccccaag
     3121 accagcagcg atagcgtcgc caatggcttt cagcgcctgt tcagcatctg caccctgggc
     3181 ggtaaagcgt aggcgatgac ctttcttaac gccaagtgcc acaactttca tcagactacg
     3241 tccgtttgcc ggtttgccgg tgccatcaag gtttgtcacg gtaatatcac tgttaaattg
     3301 tttaatggta ttgaccagca tggtacctgg acgagcatgc aggccgtgtt cattgcgcac
     3361 cacaaactcc gcgcttaaca cgtcgtcggt cggcgcatca tcgctggtca gcagcgccag
     3421 caacgttgcc gcatctgctt tcagcaagcg gtcagcttta ttgtcgagca ataaatcagc
     3481 gagacgctta agaaccgcga tgggctgatc gtcattcatc gccacactca ccagcatggc
     3541 tgccgtttcg ccgtccacag caaaagcatt tgccgcacgg cttaccgcaa tcgcgctacg
     3601 cagattgcct tcggcgctat cgctcagcca gataccctgt ccgagattca gcggttgttc
     3661 attgatggct ttggtgacga aagtggcgtc aactgccccc gcctctttca gacgcgcagc
     3721 gttcagcgcc tgaagagtca gcagatcgct ggcgacgata tccagcgtca gcatttcgtt
     3781 gtcgagcttc agctgctcac tctgcttttc gcccatcagt aatgcgcgaa gttcttctgc
     3841 tgttgttgct gacttcagtt gttcagcaac ggaatcatcg ctcagtacgt gggtcagctg
     3901 gcgtagcagg cccagatgtt catccgagct ggcagcaata ccgattgcca cgtacgctac
     3961 ctgaccgtca ccccaggtga cgccttccgg gaactgaaat acctgaacgc cggttttcag
     4021 cacctgatcg cgggtgtcgg tagtgccgtg tggaatagca ataccattgc cgaggaacgt
     4081 tgaggtttgc tgttcgcgcg ccagcatgcc attgacgtag ccttctgcta cattaccggc
     4141 ctgcaccagc gccgcagcga cctggcgaat cgcctcttct ttgtctccgg ccttttcgcc
     4201 cggatggatg tcctgtacgg ataactggaa catagttctc ctctcttgct gaattgaaac
     4261 gattcagcct ctatgagaaa aaaagcgcca acctggctta gggttaaaga caagatcgcg
     4321 ctgaaacgtt tcaagaaagc ataatacttc tgtttcagca cgcaaggaaa gccgtaaagt
     4381 tatgagcaaa agtttgatgt gctgcacatt tttttgtcat ttttcctaat tgctgatggg
     4441 aaaatccgtt atcagcaatt tcatttcagc agcgtcagca gccaatcagc aacagtgaaa
     4501 tggcattttg attattcgtg gcgcttttga tttcgctgtc tgtttatttt ctgacaagca
     4561 gcgtaaactc cgcgtcttcc tcttccagtg atagacctgc atgcataact cccccgcagt
     4621 ctccagcgcg aaatcgtttg acctgacctc gacggcgttt ttaatcgttg cctttctcac
     4681 cggtattgcg ggtgctctgc aaaccccgac actcagtatt ttccttaccg atgaagtaca
     4741 tgcccgtccg gcgatggtgg gattcttctt taccggcagc gctgtcattg ggattctggt
     4801 aagtcagttt ctcgctgggc gctctgataa gcgcggcgat cgcaaatcgc tgattgtctt
     4861 ttgctgcctg ttaggcgtgc tggcctgcac cctttttgcc tggaatcgca actactttgt
     4921 tttgctattc gttggcgtct ttcttagcag ctttggctcg accgctaacc cgcaaatgtt
     4981 tgcccttgcc cgtgaacatg ccgacaaaac cggacgtgag gcggtgatgt tcagctcttt
     5041 tttacgcgct caggtttcac tggcatgggt cattggccca ccgctggctt atgcgttagc
     5101 gatgggtttc agctttacgg taatgtatct gagcgcagcg gtagcgttta ttgtttgcgg
     5161 cgtgatggtg tggctgtttt taccgtcgat gcaaaaagag cttccgctgg cgaccggcac
     5221 ggttgaagcg ccgcgccgta accgtcgcga tacgctactg ctgtttgtca tttgtacatt
     5281 gatgtggggc tcgaacagcc tgtacatcat caacatgccg ctatttatta tcaacgaact
     5341 gcatcttccc gagaaactgg ccggtgtgat gatggggacc gccgccgggc tggaaatccc
     5401 gacgatgttg attgccggat atttcgccaa acgtctgggt aagcgtttct taatgcgcgt
     5461 tgctgccgtg ggtggcgtct gtttttacgc aggaatgctg atggcgcatt cacctgccat
     5521 tctgttgggc ttgcagctgc taaatgctat ttttattggc attctgggcg gcatcgggat
     5581 gctctatttt caggatttga tgcccggtca ggcaggttca gccaccacgc tctataccaa
     5641 cacgtcgcgc gtgggctgga tcatcgcagg atcagtggcg ggcatcgtcg ccgagatctg
     5701 gaattatcac gctgtgttct ggtttgcgat ggtgatgatt atcgccactc tgttttgctt
     5761 actgcggact aaagatgttt aagacgcggt cagcatttcc agatcgataa gccaggtcat
     5821 ggcctgctga cgcgagttac cgcacatttc agcgctggct tgtaagcctg cacagacttt
     5881 cggtcgcagt ggcgaggtga aaattttaca gcgttgctgt tcatcaagct gaatgcaggg
     5941 cgtattggcg ggcttgccat ctggcatacc gggaataggg ctgaaaattg aaggggcagt
     6001 gcaacacgcc ccgcaacccg gacggcattc catacgttgt tctcttaagg ttaaccaccg
     6061 gattgatgag cgcgcacagt accacctttt ttgcaccagc aaaagtgcga ataccacttg
     6121 cccgaaaggc ccgtcgcgag tactttgtcg cgatattttt gacattttcg actacaggaa
     6181 tttttcgatg ccaagagcga acgaaattaa aaaaggtatg gtactgaatt acaacggcaa
     6241 actgctgctg gttaaggata ttgatattca gtcgcccact gcccgcggtg ccgctacgct
     6301 gtacaaaatg cgtttttctg atgtccgtac cgggctgaaa gtagaagagc gtttcaaagg
     6361 tgatgatatc gttgacaccg tgacgctgac ccgccgttac gttgacttct cctatgtcga
     6421 tggcaacgaa tatgtcttta tggataaaga agactatacc ccgtatacct tcaccaaaga
     6481 tcagattgaa gaagagttgc tgtttatgcc agagggcggc atgccggaca tgcaggtgct
     6541 gacctgggac ggtcaactgc tggcgcttga gctgccgcag accgttgatc tggaaatcgt
     6601 tgaaaccgca ccaggcatca aaggggcatc tgccagcgcc cgtaacaaac cggcgacatt
     6661 gagcactggt ctggtgattc aggtaccaga atacttaagc ccgggcgaaa aaattcgtat
     6721 ccatatcgaa gaacgccgtt atatggggcg tgctgactaa cttcagccgc atgcagaaaa
     6781 gggatagctc aggctgcccc ttttttaatt tattatacca atcttctatt ttgcgcttcc
     6841 tgaacacctt acgccctcaa ttttcactcg ttgattgatc gccctcacac ttcatcgcat
     6901 taacaatcca gaccaatttc aattgctgtc atataacttt acactgtcat tgttaattta
     6961 tcgttactaa gacgtgactc ctatgaagac aattgcctcc gttacgctcc cgcatcatgt
     7021 acacgctcca cgctacgatc gccagcagtt gcaatcacgt atcgttcatt ttggctttgg
     7081 cgcctttcac cgcgctcatc aggcgttact gaccgatcgt gtgctgaatg cccagggcgg
     7141 cgactggggg atctgtgaaa tcagcttgtt cagcggtgat caactgatga gccagctccg
     7201 cgcacagaac catttatata ccgtgctgga gaaaggtgcg gacggcaatc aggtgataat
     7261 tgtcggtgcc gttcacgaat gccttaatgc aaaactggat tccttagcgg caattattga
     7321 gaaattttgc gagccacagg tggcaattgt ttccctgacg attaccgaaa aaggctattg
     7381 tattgacccg gccaccggtg cactcgacac cagtaatccg cggattattc acgatctaca
     7441 aacccctgaa gaacctcact ccgcaccggg tattctcgtc gaagcactga aacgccgccg
     7501 tgagcgcggc cttacaccgt ttaccgtgct ctcctgcgac aatattcccg acaatggcca
     7561 tgtggtgaaa aacgcggtgc tgggaatggc agaaaaacgt tcgccagaac tcgccgggtg
     7621 gataaaagaa cacgtcagtt ttccgggaac catggtcgac cgcattgttc cggctgcaac
     7681 cgacgaatca ctggcagaaa tcagccagca tctgggggta aatgatccct gcgcgattag
     7741 ctgcgaaccg tttatccagt gggtggtgga agataacttc gtcgctgggc gtcctgcctg
     7801 ggaagtcgca ggtgtacaaa tggtgaatga tgtcctgcca tgggaagaga tgaaactgcg
     7861 tatgcttaat ggcagccact cttttctcgc ttatctgggt tacctttcag gattcgccca
     7921 tatcagtgat tgcatgcagg atcgcgcatt tcgccatgcc gccagaacat taatgctgaa
     7981 tgagcaagcg ccgacactac aaattaaaga tgtcgattta acacaatatg cggacaagtt
     8041 aattgctcgt tttgctaatc cggcgctgaa acataagacc tggcaaatcg caatgaatgg
     8101 tagccagaaa ttaccgcaac gtatgctggc aggtattcgc atacatctgg ggcgcgaaac
     8161 ggactggtcg ttgctggcat taggcgttgc tggctggatg cgttacgtca gcggcgttga
     8221 tgatgccgga aatgccattg atgttcgcga tccgcttagc gataaaattc gcgaacttgt
     8281 tgcgggcagc agcagtgaac aacgcgtaac cgccctgctt tccctgcgtg aagttttcgg
     8341 tgatgatctg ccagataacc cgcattttgt gcaggccatc gaacaagcct ggcaacaaat
     8401 cgtacaattc ggcgcacatc aggcgctatt aaacaccctc aaaatttaac gatttctgcg
     8461 gttaaagcgg atgaagctca ccttcgtccg ctctcccctt ctcttttctg ccttttttag
     8521 ccaggattaa cgctcagtta acttaccaga gtattgcgga gccgttgtga ccaggaccaa
     8581 cctcatcacc ggttttctcg gcagcgggaa aaccacgtcg attcttcatc tgttagccca
     8641 taaagatccc aacgaaaaat ggacggtact ggttaatgaa tttggggaag tcggaattga
     8701 tggtgctttg ctcgccgata gcggcgcatt gctgaaagag atccccggcg gctgcatgtg
     8761 ctgcgttaat ggtttaccca tgcaggtagg gttgaatacc ttactgcgtc agggaaaacc
     8821 agaccgcttg ttgatagagc cgaccgggct gggccatccg aaacagatcc tcgatctgtt
     8881 aaccgcacca gtctatgaac cgtggataga tctgcgcgcc accttgtgca ttctcgatcc
     8941 acgcttgctg ctggacgaaa aaagcgccag caatgaaaac ttccgtgacc agctggctgc
     9001 cgcagacatc attgtcgcca ataaatccga ccgtgcgacg cccgaaagtg agcaagcgct
     9061 acagcgttgg tggcagcaaa atggtggcga tcgacaatta attcacagtg agcatgggaa
     9121 agttgacggt catcttctgg atttgccgcg tcgcaattta gccgagttgc ccgccagcgc
     9181 cgcgcattct catcagcatg tcgtgaaaaa agggttagca gcgttaagcc tgccagagca
     9241 tcaacgctgg cgtcgcagtc tgaacagcgg gcaaggatat caggcctgcg gctggatatt
     9301 cgacgctgat acggtattcg acaccattgg cattctggaa tgggcgcgac ttgcaccggt
     9361 ggaacgcgtc aaaggcgtgc tgcgtattcc cgaagggctg gtacgaatca accgtcaggg
     9421 cgatgacctg cacattgaaa cgcaaaacgt tgcgccaccg gacagccgta ttgagctgat
     9481 ttccagcagc gaagctgact ggaatgcctt gcagagcgcg ctgttgaagc ttcgtttagc
     9541 gactaccgcg taaggttgcc tgcgtttttc agtaagataa ttagagaaaa tatgattaaa
     9601 aatttgccgc aaatagtgtt gttgaatatt gtcggcctcg cgctgtttct ttcctggtat
     9661 attcccgtta atcatggatt atggttgccg attgatgcgg atatttttta tttctttaat
     9721 cagaaactgg tcgaaagtaa ggccttttta tggctggttg cattgaccaa caatcgcgct
     9781 ttcgacggtt gttctctgct ggcgatggga atgttgatgc tgagtttctg gctgaaagaa
     9841 aacgccccag gcagacgacg tatcgtgatt attggtctgg taatgctatt aactgcagtg
     9901 gtattaaacc agctgggtca ggcattaatt cctgtaaaac gggccagccc aacattgacg
     9961 tttactaata ttaaccgcgt cagcgaactg ctctctgtcc ccacgaaaga tgcctcacga
    10021 gatagctttc ccggcgatca cggcatgatg ctgcttattt tttcggcatt catgtggcgt
    10081 tatttcggta aagttgcagg tcttatcgcc cttattattt ttgtggtttt tgcgtttccc
    10141 agagtaatga ttggcgcaca ctggtttact gacatcattg tcggttcgat gaccgtgata
    10201 ttgatcggtt tgccctgggt gttgctgacg ccattaagtg atcgattaat cacctttttt
    10261 gacaaatcac taccgggaaa aaacaaacat ttccaaaaca aataactcac agtaattaac
    10321 atcatcaggt ttatttttat agtgaggata atcctgatga tgcgcaccgt gctttcatct
    10381 atcgaacgca aaaatcattc tctaagtaaa tgaatggatt gcatgcgttt cactcaatcg
    10441 tactttaatt gaccaatccc gcttattaac tttctgtatc actttttctt ataaaaaatc
    10501 atgtaaaacc gctcgccaag accgcaccaa tcgggtaatc tcgaactcgt tttgcctcgg
    10561 cggtagatta tcctcacagc atataatttt gtgcgttagt ccacagattt ggccttaagg
    10621 aattgtttca acatgcccag gtaattagtc tcgtgtcgct tggcattttt ttataacgat
    10681 atttgtcgtt aaggacttca agggaaaaca aacaacatgg tcaaatctca accgattttg
    10741 agatatatct tgcgcgggat tcccgcgatt gcagtagcgg ttctgctttc tgcatgtagt
    10801 gcaaataaca ccgcaaagaa tatgcatcct gagacacgtg cagtgggtag tgaaacatca
    10861 tcactgcaag cttctcagga tgaatttgaa aacctggttc gtaatgtcga cgtaaaatcg
    10921 cgaattatgg atcagtatgc tgactggaaa ggcgtacgtt atcgtctggg cggcagcact
    10981 aaaaaaggta tcgattgttc tggtttcgta cagcgtacat tccgtgagca atttggctta
    11041 gaacttccgc gttcgactta cgaacagcag gaaatgggta aatctgtttc ccgcagtaat
    11101 ttgcgtacgg gtgatttagt tctgttccgt gccggttcaa cgggacgcca tgtcggtatt
    11161 tatatcggca acaatcagtt tgtccatgct tccaccagca gtggtgttat tatttccagc
    11221 atgaatgaac cgtactggaa gaagcgttac aacgaagcac gccgagttct cagccgcagc
    11281 taataaaccg tttggatgca atcccttggc tatcctgacg agttaactga aagcactgct
    11341 taggtagtgc ttttttgttt tcattcatca gagaaaatga tgtttccgcg tcttgatcca
    11401 ggctatagtc cggtcattgt tatcttttaa atgttgtcgt aatttcagga aattaacgga
    11461 atcatgttca tacgcgctcc caattctgga cgtaagctcc tgcttacctg cattgtagca
    11521 ggcgtgatga ttgcgatact ggttagctgc cttcagtttt tagtggcctg gcataagcac
    11581 gaagtcaaat acgacacact gattatcgac gtacaaaagt atctcgatac ctattttgcc
    11641 gacctgaaat ccactactga ccggcttcag ccgctgacct tagatacctg ccagcaggct
    11701 aaccccgaac tgaccgctcg cgcggcgttt agcatgaatg tccgtacgtt tgtgctggtg
    11761 aaagataaaa aaacattctg ttcatctgcg actggtgaga tggacattcc actcaatgaa
    11821 ttgattccgg cgctcgacat taataaaaac gtcgatatgg cgatcttacc cggcacgccg
    11881 atggtgccga acaaacccgc aatcgtcatc tggtatcgca accctttgct gaaaaatagc
    11941 ggtgtgtttg ccgctctgaa tctcaacctg acgccttctc tcttttatag ttcacggcag
    12001 gaagattacg atggcgtcgc cctcattatt ggcaatactg cgctatctac cttttcttca
    12061 cgtttgatga acgttaatga attaaccgac atgcctgtcc gtgaaactaa aattgcgggc
    12121 attcctctga ccgttcggct ttatgcggat gactggacat ggaacgatgt gtggtacgca
    12181 tttttactgg gcggcatgag tggaactgtc gttggcctgc tctgctatta cctgatgagc
    12241 gtacgtatgc gccccggcag agaaatcatg accgccatca agcgcgaaca attttacgtg
    12301 gcgtatcaac cggtggtgga tacacaagct ttgcgagtaa cgggcctgga agtactgcta
    12361 cgctggcggc atcctgtcgc gggagaaatt cccccggatg ccttcattaa ctttgccgaa
    12421 tcgcaaaaga tgattgtgcc gctgactcag cacctgtttg agttaattgc ccgcgatgcc
    12481 gcagaattag aaaaagtgct gccagtaggc gtcaaatttg gtattaacat tgcgccggac
    12541 catctgcaca gcgaaagctt taaagcagat atccagaaac tgctcacttc cctgcccgca
    12601 caccatttcc agattgtgct ggaaattacc gagcgcgata tgctgaaaga gcaagaagcc
    12661 acacaactct tcgcctggct gcactcggtc ggcgtagaaa ttgctattga tgacttcggc
    12721 accgggcaca gcgcgcttat ctatcttgag cgttttacgc tcgattatct gaaaattgac
    12781 cgtggattta tcaacgccat cggtacggaa acgatcactt cacccgtact tgacgcggtg
    12841 ctgacgctgg cgaaacgcct caatatgctg acggttgctg aagggggcga aacgccggaa
    12901 caggcgcgat ggctaagcga acccggcgtt aatttcatgc aaggctactg gattagtcgc
    12961 ccgttaccgc tggacgattt tgttcgctgg ctgaagaaac cgtatacgcc gcagtggtaa
    13021 ggtgtgctta cgtcccttat tattcatagt gaaagcatgc cggattgcgg ctaatgatga
    13081 gtaaaaggaa atccgttgca gatgattgtg cgcatactgc tgctgtttat cgccctgttc
    13141 accttcggtg cgcaggcgca ggctatcaag gaaagctatg cctttgccgt gctgggcgaa
    13201 ccccggtacg catttaattt caaccatttt gattatgtga accccgccgc gccaaaaggt
    13261 gggcagataa cgttgtcagc cctcggtacc ttcgataatt tcaaccgcta tgcactgcgc
    13321 ggcaacccgg gcgcacgcac cgagcagttg tacgacacgc tatttacgac ttccgatgac
    13381 gaaccaggca gttattaccc gctgattgct gaaagtgcac gctatgctga cgattattcc
    13441 tgggtggagg tcgctattaa tccgcgcgcc cgttttcatg atggttcgcc cattactgcc
    13501 cgcgatgtag agtttacttt tcaaaaattt atgaccgaag gcgtgccgca atttcgtctg
    13561 gtctacaaag gcaccaccgt caaagccatt gcaccgttaa ccgtgcgcat tgagttagct
    13621 aaacccggca aagaagatat gctgagtctg ttttcgctgc cggtatttcc agaaaagtac
    13681 tggaaggatc acaaacttag cgacccgctc gccacgcctc cgcttgccag tggtccgtac
    13741 cgcgttacgt cctggaaaat ggggcaaaat attgtctatt cccgtgtgaa agattactgg
    13801 gcagcaaact taccggtaaa ccgtggacgc tggaatttcg acaccattcg ctacgattat
    13861 tacctcgatg ataatgtcgc ctttgaagcg tttaaagcag gtgcctttga tttgcgtatg
    13921 gaaaacgacg ctaaaaactg ggccacgcgt tataccggta aaaatttcga taaaaaatac
    13981 atcatcaaag atgagcaaaa gaacgaatca gcccaggata cgcgctggct ggcgtttaat
    14041 atccaacgtc cggtattcag cgatcgccgg gtccgggaag caatcactct cgcctttgac
    14101 tttgaatgga tgaacaaggc gttgttttac aacgcctgga gtcgcacgaa cagttatttt
    14161 cagaataccg aatacgcggc cagaaattac cccgacgccg cggagctggt gcttctggca
    14221 ccaatgaaaa aagatctacc gccagaagtc ttcacacaaa tctaccagcc gccggtatct
    14281 aaaggcgatg gctacgatcg tgacaacctg ttaaaagccg acacacttct caacgaagcg
    14341 ggctgggtgc tgaagggtca gcaacgcgtt aatgccacaa cgggtcagca actcagcttt
    14401 gaattattgc ttcccgcaag cagcaatagt cagtgggtat tgccgttcca gcacagcctg
    14461 caacggctgg gtatcaacat ggacattcgc aaggtggata actcgcaaat caccaaccgc
    14521 atgcgcagtc gcgactatga catgatgccg cgcctatggc gggcgatgcc gtggcccagt
    14581 tccgatttac agatttcctg gtcatcggaa tatatcaatt ccacttataa tgcccccggc
    14641 gtgcaaagcc cggttatcga ctcactgatc aaccaaatta ttgccgcgca gggaaataaa
    14701 gaaaaattat tgccgttggg gcgagcactg gatcgcgtat taacgtggaa ttattacatg
    14761 ctgccaatgt ggtacatggc ggaagaccgt ctcgcctggt gggataaatt ctcccacccc
    14821 gctgtacgcc ctgtttacag cctgggtatc gatacctggt ggtatgacgt taataaagcg
    14881 gccaaactgc cgtcagccag gcaacaggga gagtagatgg gcgcttacct gattcgccgt
    14941 ctgttgctgg tgatcccaac attatgggcg attatcacca tcaacttttt catcgtgcaa
    15001 attgcgcctg gcggtccggt cgaccaggcc atcgccgcca ttgagtttgg taatgccgga
    15061 gtattacccg gcgcaggcgg tgaaggtgtt cgtgccagcc atgcgcaaac gggtgtcggc
    15121 aatatcagcg acagtaatta ccgtggcgga cgcggattag atccagaagt gatcgctgag
    15181 atcactcatc gctacggttt tgataagccg atccacgaac gttacttcaa aatgctctgg
    15241 gactacatcc gctttgattt tggtgatagc ctgtttcgca gcgcctcggt gctgacgctg
    15301 attaaagaca gtctgccggt ttccatcacc ctcggattgt ggagcacgct gattatctat
    15361 ctggtgtcga ttccgttagg cattcgcaaa gctgttcata atgggagccg ctttgacgtc
    15421 tggagtagcg catttatcat tatcggctac gccattccgg cctttttgtt tgccatcctg
    15481 ctgattgtct tcttcgcggg cggcagctat ttcgacctgt tccctctacg cggcctggtt
    15541 tccgctaact ttgattcgct gccgtggtat cagaaaatca ccgattatct gtggcatatc
    15601 acgctgccgg tgctggcgac ggtgattggt ggctttgcgg cgctgaccat actgacaaaa
    15661 aactcattcc ttgatgaagt gcgcaagcaa tacgtggtga ccgcgcgtgc gaaaggggta
    15721 agtgaaaaaa atattctctg gaaacatgtg ttccgcaacg ccatgctgct ggtgattgcc
    15781 ggttttccgg cgacgtttat cagcatgttt tttaccggct cgctgctgat tgaggtgatg
    15841 ttttcactca atggtctggg cttactgggc tacgaagcga ccgtctcgcg cgattatcct
    15901 gtaatgtttg gtaccttgta tattttcacc ctgattggcc tgctgctgaa tattgtcagt
    15961 gatatcagct atacgctggt tgatccgcgt attgattttg agggacgtta atgtcgcgac
    16021 tcagccccgt caatcaggcc cgttgggcgc gttttcgtca taaccgtcgc ggctactggt
    16081 cgttatggat tttcctcgtc ttgtttggtt tgagtttgtg ttctgagctt atcgccaacg
    16141 ataaaccatt gctggtacgt tatgacggca gttggtattt cccgttgttg aaaaactaca
    16201 gcgaaagcga ttttggcggc ccgctggcaa gtcaggctga ttatcaggac ccgtggctga
    16261 aacaacggct ggaaaataac ggctgggtac tgtgggcacc gattcgcttt ggtgctacca
    16321 gtatcaactt tgctaccgat aagcccttcc cttctccacc ctcccggcaa aactggctgg
    16381 gaacggatgc caacggcggc gatgtgctgg cacgcattct ctatggcacg cggatctcgg
    16441 ttctgtttgg cctgatgctg actctctgtt ccagcgtgat gggcgtgctg gcgggggcgc
    16501 tacaaggcta ttacggcggt aaagtcgatc tctggggaca acgctttatt gaagtatggt
    16561 cggggatgcc gacgctgttt ttgattattt tactttccag cgtcgtacag cctaacttct
    16621 ggtggctgct ggcaattact gtcttgtttg gctggatgag tctggtcggc gtggtgcggg
    16681 cggagttttt acgtactcgt aatttcgact acattcgtgc ggcgcaggcg cttggcgtca
    16741 gcgatcgcag tatcatcctg cgtcatatgt tgcctaatgc catggtcgcg accctcacct
    16801 ttttaccgtt tattttatgt agttcgatca ccaccctgac ctcactcgat ttcctcggct
    16861 tcggtctgcc gctcggttca ccgtcactcg gtgaactgct gttacaaggg aaaaataacc
    16921 ttcaggctcc gtggcttggg atcaccgcct tcttgtcggt ggcgatatta ttgtctttgc
    16981 tgatctttat tggtgaagcc gtccgcgacg catttgatcc taataaggcg gtgtagcatg
    17041 acgcaaactc tgttagcgat tgaagatttg tcggtgggtt ttcgccatca gcaaaccgta
    17101 cgtacagtag tcaatgatgt ttcactacag attgaggctg gcgaaacgct ggcgctggtg
    17161 ggtgagtcag gttcaggcaa aagcgttacc gcgctgtcaa ttttacgcct gctcccttcc
    17221 ccgccggttg aatatctctc cggcgatatt cgttttcgtg gcgaatcgct gcttcatgcc
    17281 agcgatcaaa cgttgcgcgg tgtacgcggt aataagatcg ccatgatttt tcaagagccg
    17341 atggtgtcgt taaatccatt gcataccctg gaaaaacagc tttatgaagt gctttcactc
    17401 caccgcggga tgcgtcggga agcggctcga ggcgaaattc ttaactgcct tgatcgcgtt
    17461 ggtatccgcc aggcggcaaa acggctgaca gattatccgc atcagctctc cggcggcgaa
    17521 cgccagcggg tgatgattgc gatggcgctg ttaacgcgac cggaattatt aattgccgat
    17581 gaaccgacca ccgcactgga cgtctctgtc caggcgcaga ttttacagct gttgcgcgaa
    17641 ctgcaaggcg agctgaatat gggcatgctg tttattactc ataacctcag cattgtcaga
    17701 aaactggccc accgcgtggc ggtaatgcaa aacggtcgct gtgtcgagca aaattacgcc
    17761 gctacgctat ttgcctcacc cactcatcct tacacacaaa agctactcaa cagtgaaccg
    17821 tcaggcgatc cagtgccgtt gccagaacct gcctcaacgt tgctggatgt tgaacagctt
    17881 caggttgcct tccccattcg caaagggatt ttgaagcgca ttgtggatca taatgtggtg
    17941 gtgaaaaaca tcagttttac gctacgagcg ggtgaaacac tgggtttagt gggcgagtcc
    18001 ggttccggga aaagtacgac gggactggcg ctgctgcgac tgattaattc tcagggcagc
    18061 atcatctttg acggtcagcc actgcaaaat ttaaatcgcc gccagctgtt acctattcgt
    18121 catcgcattc aggtggtatt tcaggatcca aactcctcgc tcaacccacg actcaacgtt
    18181 ttgcagatta ttgaggaagg cttacgggtt caccagccga cgctttctgc cgcacaacgc
    18241 gaacaacaag tgatagccgt gatgcatgaa gtgggattag atcctgaaac acgccaccgt
    18301 tatccggcgg agttctctgg tggtcagcga caacgtattg caattgccag ggcattaatt
    18361 cttaagccct cgctgatcat acttgatgaa ccgacatcat cactcgacaa aacggtacag
    18421 gcgcaaatat tgacgctatt gaaatcattg caacaaaagc tgcaactggc ctatttgttt
    18481 atcagccacg atttgcacgt tgtccgcgcg ttatgtcatc aggttatcgt actgcgacaa
    18541 ggggaagtag tggaacaagg accgtgcgcg cgcgtgtttg ccgcaccgca gcaggagtat
    18601 acgcgtcagc tactggcgtt gagctgacgc ttaaaaagga ttgtagtttg aaaagggttc
    18661 ggcaattgcc acaccaaaat ttttcaatcg gcatgttgca gcaaactcgt cctggcgatt
    18721 tacaaacagg cacggctcac cttcacactc cagcaccgaa cacggaactt cgatgtcgct
    18781 taacgcctgg cttagtttgt acatcaccga ccatgcatta tcaccatccg ggctaagcaa
    18841 tttaagcgcc accaggctgt tgtcaccgca cggtccattt tgcggaatcg gttcaacctt
    18901 cgaacctgcg aaaccagcag accagcgaaa gttctgcggt agacgatgga cgattgagag
    18961 ttgtaatgaa gtcacttatt ttccccggaa gcacatttac ttcacaattt gtttacatca
    19021 attttaacac atcatcaaca atccgtcttt atacagatcg taaagattga tgcctcgcct
    19081 gcgtggcctc tatggctctg atttaagtaa tatctgcggg cgtggtcacg tttgcggggc
    19141 tatatgtacc cgtttctgcg atctaactca acctttttaa ctacaatgat gtgacttttt
    19201 acataaattg attttacata aaataaacat atatcggggg aatgatagat ttgtggttga
    19261 catgcatcaa caaaggaagc cttttagctt cctcgttgtg caatagatca ccgttttttc
    19321 ggtcgactgg cgtacagaca gaagagaatg gagctgcttg cgcagaatgc aattgaccaa
    19381 atcatcggcc atgcagagtt aaaggtcgca agagaaagca atgcgccaac aattgcccct
    19441 atgccaaaac ggaaggttcc tgccagcgaa gatgccgttc ccgccatatg gggaaactca
    19501 tcaagaatga ccgccatcgc attggatgac accatcgaca cacagcccac aaacgccgca
    19561 acgccaacca ccagaatccc agccccagca gcgcactgat gaccatccac gctgccataa
    19621 taaattgtat ccacaacccc gagcggaaca tatttaacgc gccaatgcgg cgaacgaagc
    19681 ggctgttaaa gatggtcatc acgaacagga aaacaatgtt tagtgcaaag taataaccaa
    19741 agttttccgg cgcgacgtgg ttaatttcaa tgtagacaaa cggtccggcg ctcaggaacg
    19801 agaacattcc ggcaaagctg aaaccgctgg ccagcatata actcaggaca cgcttatgac
    19861 ggaacagcgc cgcaaagtta ccaatagtag tacgaatgtg aaaaggctgg cgacgctccg
    19921 gtggtaaggt ttctttgatc agaaagaaga tcattgccga agccagaatc gccgccacgg
    19981 cgaggatcca gaagatgtaa tgccagctca gccacaccag cacccagccg ccaactatcg
    20041 gtgccatcag cggtgcaatg gtcgtcacca gcatgacaaa cgacatcatc cgcgagaact
    20101 cttctttcgg gtagatatca cgcatcaaag cgttgataac catgctggcc gcagccgcag
    20161 ccagcccgtg gaaaaaacgc atcacaatca gctgatcgat ggtttgtgcc aacgcacacg
    20221 ccaccgcggc ggcggcaaac accagcgtac cgccgagcac caccggctta cgcccgaagc
    20281 tgtctgccat cggcccgtag attaactgcc ccaacgcaaa gcccagaata taagtactga
    20341 gggtcatctg cgtactgccc gccggtacgc caaactgcgc tgaaattacc ggtagcgcgg
    20401 gcagatacat atcaatcgac agcggcatca acatggccag caggccaagg ataaaaacaa
    20461 tagcaaacga cgaatgctgt cgggtggtca caacgggctc ctgaaagtca ttgaaaagtt
    20521 agacgacgct ggcaatttct tcttcagtta acggacgata ttcaccgggg gctaaatcag
    20581 catccagcgt aataccgcca atacgttcac gatgcagctc aaccacgtgg ttacccacgg
    20641 cggcgaacat gcgtttcacc tgatgataac gcccttcgct gatggtcaga cgaacctgcg
    20701 ttggggtaat cacttccagc accgcaggct tagtgagatc tttttcgtta tgcagctgca
    20761 cgcctttagc aaattgctct gccgtatcgt cagctacagg tgattccagt gtcaccagat
    20821 aggtcttctc gcaatgatgg cgcggagaag taatgcggtg cgaccactga ccatcatcag
    20881 tcatcagcac cagaccggtg gtatcgatat ccagacgtcc tgcggcgtgc agtttccagg
    20941 ctaccggttc atccagaaaa tagagcaccg ttgggtgatc agggtcgtcc gtggagcaaa
    21001 catagccctg aggcttattg agcatgaagt aacgtggacc atgttgctgc gccagcgggt
    21061 tgccatcgta agcgacatca tgttcaggaa gcagtttgaa cgctgcatta cgaacaattt
    21121 cgccatcgac ggtgacacga ttgccgcgga tttcacgccc ggcaatagca cggctaacgc
    21181 cgagttgctg tgcgataaat ttatcaagtc gcatgtgtgt gattttgcct gtaaaaacgg
    21241 aggtcgggca ttgacccgaa aatctgaact gttgtctgcc cagtatagcg gtctaataac
    21301 atccctcaag ggaaaaagat ccatggcata ctattagcag aataatctac ctacgcgaga
    21361 ccatgatttt tacactccgc ccatatcagc aagaagccgt ggatgccacg ctcaaccatt
    21421 ttcgtcgtca taaaacccct gccgttatcg tgctgcccac cggcgcaggt aaaagcctgg
    21481 tgattgccga actggcacgg ctggcgcgtg gtcgtgttct ggtgctggca cacgtcaaag
    21541 aactggtggc gcaaaaccat gcaaagtatc aggcgctggg gctggaagcc gatatttttg
    21601 ccgctgggtt aaagcgcaaa gagagccacg gtaaagtggt atttggcagc gtgcagtcgg
    21661 tcgctcgtaa tcttgatgcc tttcagggtg aattttcgct gttgattgtc gatgaatgcc
    21721 accgtattgg tgacgatgaa gagagccagt atcagcaaat cctcactcac ctgactaaag
    21781 tgaatcccca cttacgcctg ctggggctga ctgccacgcc ttttcgattg ggcaaaggct
    21841 ggatctacca gttccattat cacggcatgg tacgcggcga tgagaaagcc cttttccgtg
    21901 actgcattta tgagctgccg ctgcgttata tgattaaaca cggctatctg acgccgccag
    21961 aacgactgga tatgccagta gtgcaatacg atttcagccg cttgcaggca cagagtaacg
    22021 ggctgttcag cgaagctgat ctcaaccgtg agctgaaaaa acagcaacgc attacgccac
    22081 atatcatcag ccagatcatg gagttcgccg aaaagcgcaa aggggtaatg attttcgccg
    22141 ccaccgttga acacgcaaaa gagattgtgg gattactgcc tgccgaagat gcagcactga
    22201 ttactggcga cacccccggc gctgagcgcg atgtgttaat tgaagatttt aaagcccagc
    22261 gttttcgcta tctggtcaac gtcgcggtac tgaccaccgg atttgacgcc ccgcacgtcg
    22321 atcttatcgc cattctgcgt cctaccgaat cagtgagtct ttaccaacaa attgtcgggc
    22381 gcggtctgcg tctcgctcct ggcaagactg attgcttaat tcttgattat gcgggtaatc
    22441 ctcacgacct ctacgcgccg gaagttggta caccgaaagg caaaagtgac aacgtcccgg
    22501 tacaggtttt ctgccctgcc tgcggttttg ccaacacctt ttgggggaaa acgaccgccg
    22561 acgggacatt gattgaacac tttggtcgtc gctgtcaggg atggtttgaa gatgacgacg
    22621 gccatcgcga acagtgtgac ttccgtttcc gttttaaaaa ttgcccgcaa tgtaacgcgg
    22681 aaaacgatat tgccgcccgc cgctgccgcg aatgtgacac ggtactggtt gatccggatg
    22741 atatgttaaa agcggcgcta cgactgaaag acgcgctggt attacgctgt agcggcatgt
    22801 ctttacagca tgggcacgac gagaaaggcg aatggttgaa aatcacctat tacgatgaag
    22861 acggcgcgga tgtgagtgag cgtttccgtc tgcaaacgcc cgcccagcga actgccttcg
    22921 agcagctttt tatccgcccg catacgcgca caccgggcat ccctctgcgc tggatcaccg
    22981 ccgccgatat cctcgcccag caagccttac tgcgacatcc ggattttgtc gtcgcccgca
    23041 tgaaaggtca gtactggcag gtgcgtgaaa aagtgttcga ttacgaaggt cgttttcgtc
    23101 gggcgcacga attacgcggt taatggtagt tttgattgat gtataaggcg attgagtata
    23161 gaatctcgcc cgcttttgca tacgcaaagc agatcactta cctgttgctg ggtcgcctgt
    23221 agcaggatta atttaagaga gaaagaaatg tttactatca acgcagaagt acgtaaagag
    23281 cagggtaagg gtgcgagccg ccgcctgcgt gccgctaaca agttcccggc aatcatctac
    23341 ggtggcaaag aagcaccgct ggctatcgag ctggatcacg acaaagtcat gaacatgcaa
    23401 gctaaagctg aattctacag cgaagttctg accatcgttg ttgacggtaa agaaatcaaa
    23461 gttaaagctc aggacgtaca gcgtcacccg tacaaaccga agctgcagca catcgacttc
    23521 gttcgcgctt aattgctgaa taagttgtaa aaaaccccgc tccggcgggg ttttttgtat
    23581 ctgcagatta tgcctgatta cggtattgct attttttgca ggccagataa ggcgttcacg
    23641 ccgcatctgg cattcagaat acaaaaccga ttaattgccg ccagacgtcc ggcgctgcaa
    23701 ttggtcgcgc aaattcggcg gcgtgccttt aatggtcaag gtatcggttg ctggatccca
    23761 gaagatgcgt tcacctaaca gcatcgcatc aaagttaata gtcaagccac cgccgctacc
    23821 ggcaaacttc gtcaactgac gcagtgtgct acggtctgcc gggaagctct cttccagttc
    23881 gtagcctttt tcagccgcaa acttcgtgaa gctcacttcg cttacgcctg ccagctcttt
    23941 cgacagcgat tccagttcga tctcttcgcc tgcctgcagc tgctcattac agtagctata
    24001 aacctgctgg cgcacgttct gtcgctctgc tttatccagt tgtgcttcgg cagtgaaatc
    24061 atcaaccgct tgcaacagcc cacggttttg cgctttcgcg ttcaatcctt cgctggcccc
    24121 gaggaaatcc ataaagaaat ccgccacttt gcgccctact cgccctttca ggaaagtgag
    24181 atagcgggtg gactctggat tggtttccca ttcggttaaa tcgatacgcg caacaatatc
    24241 cgcatgattg atatcaagat aatgggttgg gttgatgtcg agattttcgt taacgcgcat
    24301 actgctcagg ttgctcagaa ctgccaccag cagatactcc accgccagat aacggtagtg
    24361 gcaaaacagc acgaatccac cgtcagcaaa aggatatttc gctaattcgt cacgcaggcg
    24421 cccggttgcc gcgcggctaa atgccagaaa gtcctcttcg ccctgacgct gtaagcgcag
    24481 cgtctgcgcc agttcactct cttcgctaaa caagccgtaa gctttatttt tggcgctata
    24541 gacccgatgc agttctgcca ccatctcaac gacggtttct gtcggttcca gcaatgaatc
    24601 gcgcaacacc agctcaaggt tctgctcatc acgcttgata agctggtgca gagcaatctg
    24661 gttgatatcc agactcatga taaactctcc tttaagaccg ggcggtattc aaccaccgcc
    24721 ggttaagcga cgcaataaaa gatggcggtc attttacaga aggcagcgta acgagaatga
    24781 tttaacgcat aaaaaagcag aaaaaaaacc gttgctacgg taatatgttg ccctttcatc
    24841 aactaactga tttcgattta tgccacaaat ttcccgctac agtgatgaac aggttgaaca
    24901 attgctcgca gagctgctca acgtactgga aaaacataag gctccgaccg acctttccct
    24961 gatggtgtta ggcaacatgg tcactaacct tatcaacacc agcattgccc cggcccaacg
    25021 ccaggcaatt gccaactctt ttgcccgcgc cttacagtcc tctatcaacg aagacaaagc
    25081 gcactaaggg aaacagataa caggttatgg taactcatcg tcagcgctac cgtgaaaaag
    25141 tctcccagat ggtcagttgg gggcactggt ttgcactgtt caatattctg ctttcgctcg
    25201 tcattggcag ccgttacctg tttatcgccg actggccgac aacgcttgct ggtcgcattt
    25261 attcctacgt aagcattatc ggccatttca gcttcctggt gttcgccacc tacttgctga
    25321 tcctcttccc gctgaccttt atcgtcggct cccagaggct gatgaggttt ttgtccgtca
    25381 ttctggcaac ggcgggaatg acgctattac tgatcgatag cgaagtcttt actcgtttcc
    25441 atctccatct taatcccatc gtctggcagt tggttatcaa cccagacgaa aatgagatgg
    25501 cgcgcgactg gcagctgatg ttcatcagcg tgccggttat tttattgctt gaactggtgt
    25561 ttgcgacgtg gagctggcaa aagctgcgca gcctgacgcg tcgtcgacgc ttcgcgcgcc
    25621 cgctggccgc attcttattt atcgccttta tcgcctcgca tgtggtgtat atctgggccg
    25681 atgccaactt ctatcgcccg atcaccatgc agcgcgctaa cctgccgctt tcgtacccga
    25741 tgacggcgcg acgtttcctt gagaagcatg gcctgcttga tgcgcaggag tatcaacgcc
    25801 gtcttattga gcaaggtaat ccagacgccg tttccgtaca gtatccgtta agcgaactgc
    25861 gctatcgcga tatgggcacc gggcagaatg tgctgttgat tactgtcgat ggcctgaact
    25921 actcacgctt cgagaagcag atgcctgcgc tggcaggttt tgctgagcaa aatatttcgt
    25981 tcacgcgcca tatgagctcc ggcaacacta cagacaacgg catctttggc ctgttctatg
    26041 gcatctcgcc gagctatatg gacggcattc tgtcgacccg tacccctgcg gcgttaatta
    26101 ctgcgcttaa tcagcaaggc tatcagctgg gattattctc gtcagatggc tttaccagtc
    26161 cgctgtatcg ccaggcattg ttgtcagatt tctcgatgcc gagcgtacgc ccccaatccg
    26221 acgagcagac cgccacgcag tggatcaact ggctgggccg ctacgcacaa gaagataacc
    26281 gctggttctc gtgggtctct ttcaatggca ctaacattga cgacagcaat cagcaggcat
    26341 ttgcacggaa atatagccgg gcggcaggca atgtcgatga ccagatcaac cgcgtgctca
    26401 atgcactgct tgattctggc aaactggaca atacggttgt gattatcact gccggtcggg
    26461 gtattccgct gagcgaagac gaagaaacct ttgactggtc ccacggtcat ctgcaggtgc
    26521 cattagtgat tcactggcca ggcacgccgg cgcagcgtat taatgcgctg actgatcata
    26581 ccgatctgat gacgacgctg atgcaacgcc tgctacatgt cagcacacct gccagcgaat
    26641 attcgcaagg tcaggatttg ttcaaccctc aacgccgtca ttactgggtc actgcagcgg
    26701 ataacgatac gctggcaatt accaccccga aaaagacgct ggtgctgaac aataacggta
    26761 aataccgcac ttacaactta cgtggtgaaa gagtgaaaga tgaaaaacca cagttaagtt
    26821 tgttattgca agtgctgaca gacgagaagc gttttatcgc taactgatta attataaatc
    26881 agttagcgaa atatcttact tgcaatcggt gtggaaaacg gtagtattag cagccacgag
    26941 tcggcacgta gcgcagcctg gtagcgcacc gtcatggggt gtcgggggtc ggaggttcaa
    27001 atcctctcgt gccgaccaaa tcgcccaccg gataccaccg gaattaacag tttttagaca
    27061 cgaaaagccc gctaatcctt aattagcggg tttttttatg gtttttagtc accggacatc
    27121 accggattta taagctatca gccggatttt ttagttatac gcttagttat acgggcttgc
    27181 gtataactaa agccgtataa ctaaaaccct gttttttcgt gtggaatcat atacatatgg
    27241 cgagaaaaac acccccatta accacggtgc agatcaaagc agccagacca gcggaaaagg
    27301 aatacaccct acaggacggc ggagggcttt ttctcctggt caaaccgtcc ggatcaaaac
    27361 tctggcgatt ttcctactac cggccatcgg acaaaaaaag aatattgctg agttttggat
    27421 cgcttgatga tgtttccctg gctgatgcca gaaaacgccg tagcgagtac agagcgttaa
    27481 tcagtgccgg aactgacccg cagggccatg agaagaaaaa acgagaggca gaggcccgaa
    27541 gacaagggaa cacgttcgaa aatgtggcgg cggcatggta ccaggtgaaa atcagccaga
    27601 atctggcccc caacacgatt aaagacatct ggcgttcgct ggataaatat gtattcccgt
    27661 tcatcggcaa cacgccaata gataccctca ccgcccgaag gttcgttgaa gtgcttacac
    27721 ccatcaagga gcgcggcaac ctggaaacac tcaagcgggt tttacagcgc gttaatgagg
    27781 taatggatta cgccgccaac agtgggctga ttgatgccaa tccggctatg aatgtgcgta
    27841 aggcgtttcc ctcacctgta aaaaaacata tgccaacaat ccgccccgaa cagctgccgg
    27901 agcttatgca ggctttatca gtatcggcaa cagaacggca gaccagatta ctgattgaat
    27961 ggcagttact gaccgtaacc cgtcccgccg aagcgtcatc aacgcggtgg gatgaaatca
    28021 acctggacgc gaagcaatgg acgatacctg ccggacgcat gaagatgcgc agggatcacg
    28081 ttatcccgct ttccggtcag gctatggcgg tgctggaggc catgaaacca atcagccacc
    28141 accgcaatta cgttttccca agtctgaaag acccacagca gccgatgaac agccagacag
    28201 ctaacgcagc attgcggcgt atgggattcg ctggcgtgct ggtgtctcat ggattacgcg
    28261 ccatattcag cacagcagcg aacgaggaag gattcgagcc ggacgtaata gaggcggcac
    28321 ttgcccacgt cgacaccaac gaagttagac gggcatacaa ccggagcaac tacatagaaa
    28381 aacgcatcgt gctgatgcgc tggtggggcg aatttgtcga ggctgcggcg acgggcgtaa
    28441 ccctcgccag tggtaaaagg ggtatccgag ccgtgtagct gtacagaaaa ccagtaaaaa
    28501 ctacgaaaac catgtaaaac cgtcgtataa ttgcatcaaa tttaacgaca aggccgtgaa
    28561 acatgaaacc gttaagatgc aaaaaaatat cagatgcaat tgcgacgggc tgcaactggc
    28621 cctgatggtt cagcatgaat tttggtcaac ctacgatccg gaggacagaa cgacggcccc
    28681 atcaaaaaaa caggtagtag attttctggt atcccgtggc gcttccagaa atctggcggt
    28741 aagtattgat aaggtcgtac gtccggcatc tatgaagatc ggaggcaggc ccaaaaaatg
    28801 gcggtaacaa tcctggaagc ggcagaaatg ccgctttttt tataattcca tttcaaatca
    28861 tcaatataaa aacggtgtat accgtttaaa aacggtggga actgttttta cccatatccg
    28921 atgatttacc gtatttgtca ccggaataca ccggattcac aaggtaaatc acgatggaag
    28981 caatcagaaa aattatcttt cgccaggaag taaaaaaaat tatccacatc aaggcagaca
    29041 gcacgctgca aagcatgatc aacgccggag aatttccgca gggttttcgc gttggtttac
    29101 gccgtcgcgg atggtatgag gatgatgtgt tggcctggca gaaagaacgc gaacaggaag
    29161 cacgcggaac ggctgcttaa cgggtatcac agatatgaca aacacgaaaa aagcggcccc
    29221 gatatggagc cgcctttctg aacaattaac ccgctgcgcc ttatgtgtat atgatcccaa
    29281 acataagcac ggggatgata gccgctatca ggctggtggg caatgcaatc agtctggttc
    29341 agttcgttgc catacctgca atgagcgctt ttccctatac tctttaagga attgctcaag
    29401 ggcaaaagca catggtgcga atctttctga ttcatgctct atctttctgc gccgtctttt
    29461 ccgtgccggt gataatgttt tggtcaattc tttatcggtc attgtgttgt cctgcatagc
    29521 aatgcgccgt aatacctcac accacggcgc tgatagtttt tatcctttgg gttctatgcc
    29581 gcgccgcttt agttcagtgc gccccaactc tttaagccag ttggctaggc ttatgccgtc
    29641 gctctgtgct tctttgtcga attgctcttt tagctctgga gaaattcgca ttctgaattg
    29701 tggggattgc ccgtcccctt tagggctttt atcgcgtttg attattgaca agtggtcacc
    29761 tattgaatta gcctttccat tgttaggtgg ccaccttaac acaagagcac ttaaaaaagc
    29821 aaagccccgc aagtgtcatt accactcgca gggcttctaa ccaacaacgt aaactaggag
    29881 ccgttatggt tgccgtaaat catataccac accttgtaca cacacaaacg gcctttgtgt
    29941 ggcgttttct ggccctgagt gccggagaat ctcaaatcat ccacgtaacc gcctggacgg
    30001 aacgcgaagc gcgtagccgt tgcccgtccg gttgtgttgc tgtattcgcc gcccgtattc
    30061 gtcaggggga aacctatgca caataaaacc acaccgaacg cagcagccgc cgcactcact
    30121 acgctgatgc acgcgcttat tgatattgaa tgcactgcag agcttgcgca gggagaagaa
    30181 cagaaagacc ggacacagtt cgccctggaa tgtatccgat acatcgcaac gcggtcgctg
    30241 aatgacgcta aaaatattct tgttgctgat tgtgaaaatg gggggggtta tgcgtgatga
    30301 tcgttttaat tccctgaaac aggaatttcc cggcgttcct gatgatgcgg ctgatgcgct
    30361 ttcgtcaatg ccagaactta ttagagcggc ttttttctta ctttccacga gagaatataa
    30421 atcaacgggg cttgatgtac tgaatatcgc cgccgattat gcggaatatg tggcagaggc
    30481 gcgttacaga agaaaatttc ctgaggatgt aagccatgcg tgatatttac cacgaaacaa
    30541 tagaccgcgc atttcttgca ctttctcaca gtgaaaacat gctggaaata ttgcgcatat
    30601 ggcttgaaac acttggcgac aatgaacgcg acaaacaaaa atcaagaatt gccacggcat
    30661 taataacgct tcttgagcct gtaataatgg aactgcaaga aatagatcta ttgcacgaca
    30721 gatataaaga acagcacacc ggagaataaa aataatgaaa cttaaatatt ctggcttaac
    30781 tgccagtggc aacactcacc ctaaatttac gcgcggtgat atttaccgcg accagtacgg
    30841 cggcacggta atgattaagg gcgtggaaga acggcgtgta acctaccgcc gtgaaggtta
    30901 cgaatatgat tgcgtgatgc ctgtttatca gttccggcgt gatttttctc tggtacagac
    30961 cgcgccgcat aacgtgccca ccagcaacgc cagggcacgg gcaaacatcc agaagctgaa
    31021 aaccatgatt aacggattca ggggcaagaa atgaaaagcg caccgaactt aaaaaaacag
    31081 ccttacgaca agatgaccga agtcattatt tttgcgggta gtgatgcctg ggcacatgcg
    31141 aaacagtggc aggaacagga cgggcgactg gctggcgata atgtgcctcc cgttgtgctg
    31201 gctgatgatc aactggatga actggcagac ctgagaatca tcgacgaggg gcgctattgt
    31261 gtccggctgt acaaggcagg ccacatcagg ccatcaaata ttaatgccat tgcgcacaag
    31321 ctggcggcgg cgggtgtaac tgatgcgaat tattaccccg aagggatgca cagccatatg
    31381 cgggagaact ggcgcgaata cctggaacgg gtgcgcggga aagagccggt ggaagaaaaa
    31441 aaccaccagc gaaaaaccac gctaccgatg agcgttggat ctaccggata cgacacgcaa
    31501 ctggattacg tggttaaggg gattattccg gcggtatcgc tatgcagcat atacggggct
    31561 agcgggtcct ataaatcatt ccttgccgga tcgtgggcgt gccatgttgc cactggtcgc
    31621 cagtggggag gccgcagggt tgcacatggt gcggttctct atgtggttgg tgaaggcggt
    31681 atcggcgttc cgcgtcgtgt aaaagcctgg gaggttgtgc acgatgagca ggtgaaaaat
    31741 ctgtatctgg taaaccgccc catctttccg gctgccccgc ttgatgttga tgaaatggtt
    31801 atcgctgccc gtcaggtgga gcgggaaacg ggtaaacctg tacgcatgat tattctggat
    31861 acgctggcgc gttgctttgg tgggaatgat gaaaatgatt cccgtgatat gggggcgttt
    31921 atccgtggtt gtgacgaact gaaacgacgc acaggggcca cggtgctggt ggttcaccat
    31981 tccggcaagg atgagacgaa aggcgcgcgc ggttccagtg catttcgtgc ttcgctggat
    32041 gctgaatacc ggatacgcag ggaggacgca ggaagcgaag cgctggttat ctcatgcacc
    32101 aaaatgaagg acgcggagga actcaaagaa gccgcatatg acttacgcgt ggtggagctt
    32161 tttaccgacg ctgacggtga attaatcacg tcgctggtgg tggtggatga tccgcgccct
    32221 cctgttgaac tggagcgcat cgaggaggca gggaacaaga cggaaaacca taccgcgcta
    32281 tgggggtgca tccgttcacg cacacagaac ggcgacaagt gcacgatccc gctgttacgt
    32341 gatgacatga aaaagctggg gtatgaaatg aaaaacttcc ggcgctggct gtacaagctg
    32401 gaaaaagatg gggttattag tatcgatggg gatgatgtag cgccgctata aaagtgagga
    32461 gcaaaagcga gggggataga aagagggcca aaattagccc gctctccctc acttttcgac
    32521 ctgtatacat cctcaaaagt gaggggtaaa aaatacttat aaaacacaca catagaaaaa
    32581 ccgaaaatcc caactgcgac gaagtgagac gcttgaaaaa gtgaggcgaa aaagtgagag
    32641 gttgcgagaa atgacccaaa aacgcagaga cagaacagag ccaaaatata aagcgttaga
    32701 catgactgag cacaccttaa aggtggcaat cagaacgata gaccgccaca cgcgggaagg
    32761 atacgcgaag gaacatcccg acctgataag cgcattcatg accacggcgg cggcaaactt
    32821 tgccacgctg acagaacggg agattgccga agcggaacag gtaacaacca tcaacgttaa
    32881 aaccggagag gtggaattat gacggcacag atagccgctt acgggcggct ggtggacgac
    32941 ccgcaggtaa aacagaccag caagggcaca ccgatgacgc tggcgcgtat ggcggtatct
    33001 ttgccatgca gccaggcaca ggacggacag gcgacgttat ggttatcggt catcgcattt
    33061 ggcaaacagg ccgacttcct ggctaaacat caaaaaggcg atgttgccag cgtatccggc
    33121 acgatgcagg taagccagtg gaccggacag aacggagaaa cgcggcaggg ctggcaggtt
    33181 atcgcagaca gcgtaatcag tgcccgcgcg gcacgtccag gcgggaacag acgcaaaacc
    33241 acaggcacac agggtaatca gccaccagcg ggaggcgatg acccttacgg tgatgatatt
    33301 tcgttctgag ggggtgacga tggtacatga ccgcatagcg gaggaactgg aggcgaaagg
    33361 cttttaccgg agggcggcgg cgcgatgggg tgaagtcatg cagctggtgg agacagacaa
    33421 ggaacggcat cacatcacga tgcgacggct ggaatgttca aggaaggcac agagggcacc
    33481 ggagccgccg gataatttcg gagacctgaa aaaggcagtc gatcgcactt atgccgaaat
    33541 gggtatagat ggtgctggtg atgaaatatg gcgcaattac caggacagct aatcaaacag
    33601 ccggagaaat ccggcttttt tgcgcccaaa aaaagcccga taaggtcaga gggttcttat
    33661 cgggcttttg catatgaggt ttttggatgc actggcgttc gtgatcggga taatcatttc
    33721 ataatttgca acataactca atattattgc ataaaatgca attctgatta taatcaggac
    33781 tggataaaca tccagttatg atttttttag tcgaagagga atttcttact atggctgaag
    33841 agaaaaaagg cggtgtttcg gtgtacataa gccccgaaat cgtggaggtg ctcaagcagc
    33901 gccacaaaaa aaactatgag gctggcgtgg cggctggact ggatccgctg atgacgccgg
    33961 agccgtcgat aggttcactt gtacgctctt atttacttgc ggcgcttggg atgcataaaa
    34021 attatggggg tgaataatgg caggcaaagc aacggcactt aacactaacc agctttttat
    34081 gtacctgaat cgcggggata ttgcggattt taaattcagc cctctgttta ccacgctgtt
    34141 tttcccgaac gtggcgacat tcagcacgca aaacatcatg ctggataccc tggacattga
    34201 agaagtcacc atgtcggcgt tttgttcgcc tatggtgggt agccaggttc agcgcgataa
    34261 agggtacgaa accagcacaa tcaaacctgg ctacatgaag ccaaagcacg aaatcgatcc
    34321 aacgaaaacc atcatgcgca tggctggaga agatccggca cagcttaacg accctaccta
    34381 tcgccgtatg cgcctgatta ctggcaacat gcgccgccag ataaacgcca ttaaggcacg
    34441 cgtggaatgg ctggcggtga atgcggtaac gaccggaaaa aacatcattg agggcgaagg
    34501 catagaacgc tatgaaatcg actggaagat accggaaaaa aacatcatag agcagtccga
    34561 cggcaaaaaa tggtccgagc atgataaaga tatttacgat ccaatctatg acatcgaact
    34621 atacgcagat caggcaggtt gccccgccaa cgtcatgatt atgggcgctg aggtatggcg
    34681 cacgttacgc agctttaaaa aattccgtga actgtacgat ctttcccgtg gttcagaatc
    34741 cgccgcagaa atggcctgta aaaacctagg cgaagtggtg agctttaaag gctatctggg
    34801 cgatattgct cttattgtct attccggcaa atacactgac agcgacggca ccgaaaaata
    34861 tttccttgag cctgatttgc tggtcctggg caacaccaac aataaagggc tggtggccta
    34921 tggtgcgatt atggatcagg aagcggtaag aacgggcgca acgcaaaaca tgtactaccc
    34981 gaaaaactgg attgaggacg gcgatccggc gattgagtac gtgcagacac acagcgcacc
    35041 gcagccggtt ccggcagata ttcgcaaatt tgttaccgtc aaaattggtt aacgggggat
    35101 tatatggaca ctccatatat tgagttattt gcaggcagtc agcaggtatc aacgacactg
    35161 gtacattttg ccgctgatgc tggcgttatt caggaattta ccccgctgat gctggtggac
    35221 aatggcgagt ttaagccgtg ggatggtcag gaatctggca aggctgttta tctgacttcg
    35281 tacctcgtgg acacgtcaaa gcagaaatca gcacagtgtt acaagacggg gatattcaat
    35341 attgccgccg ttaactggcc tgagagcgtc gacaccgatg cgaaaaaatg cgccgccttt
    35401 gcgggttctg gcgtatccgt tcagccgctg gcccgataag cagggggaac gatggcaacg
    35461 aatgaaagca tcatggcgct accgctggcg agtaaattta aagccgaagc gcgggcaatg
    35521 gctgacagag gtttatcaac ctacgaggcc gtatatcaac tcaacaaact ggaagagcag
    35581 gacaagccgc gcgctgatgc gattatggcg cttcatgagc ataaagacta tcagccgctg
    35641 ttgcgtgcaa tggcaaacgt gccttgtatc gatgttgata cggctaaaaa catcctgaac
    35701 atgaccatag agcaggaacg cccgaaggtt gcaccagagc ttaccgcagc ctttgaaaac
    35761 tttatggaca tgcacagccc acaaaccgta tcagctggca tggcatacga tggcagaaac
    35821 ccaggcgatg acggcgacat cgatcgcata ctgaaaacca tctgagacaa ggccggagaa
    35881 atccggcttt tttgcgggtc cttcctggaa ttatggcccg ttacggggcg gcgacctcgc
    35941 gggttttcgc tatttatgac gtttttccgt gaaggtgaca ccaccaccac ttgattaata
    36001 tttaaccatg cagttaaggt aacattatga ttgataaagc ttgttttgta agtcagcagg
    36061 aaatagctga acatttcaag gttaacagaa ccactattcg cgcatggacc aaacagggga
    36121 tgccgtatct taatgcggat cgcggaaagt ctggcggtta tcacatcggg catacattgc
    36181 tttggtcttc aggtaaaagc cgtcttgagg ccatcagata tcacgtagaa accagtgcgc
    36241 tggaaaaaat tatgtttgcc aggctgcttt catctgagcg tgacgagtac tccagtgaag
    36301 aaacagaaca tcgatttgat gaaggtttgc agatttacgg ctattcaccg gaagatgtga
    36361 gcaaggcacg aaataaaatg gctggctttc tggctgggtg gcgtcatgcc gtaagcgttc
    36421 gccgtgccag catggagcaa tcagccgata cagaacagta aagccgataa cttgttaatc
    36481 actcgcagca atgcatgtga tttgtgcaca ctttcggtta actggttcga tgtccggttt
    36541 tagtgtctgt tttttgcgca tgtccggttc atggaaagca tgtttttata tttttcatat
    36601 ggttaacttg tagagaaacc ggacatggat cccgaaaaat tttcataaat agtgaaaacg
    36661 cgcgaggtcg ccgccccgta acgggtccat aattccagga aggacccgac gacaccagac
    36721 aatcagaacg ataggtgcac aatgacagaa gccgaaatac tgggattaat ccgccgtgct
    36781 ggtggaatca gccagcagac tgacgaacag gccacgcagc cggacagcgt gacagccgaa
    36841 aattatgcgc gtgttgttgc tgaggtgatg cgccgtgatg gtatccagct taatgatgtg
    36901 gatatgcgca acatacggat ccgcgttctt gaaatgctgg cctacaatcg ccgcgtggca
    36961 ctgtatcggg aaacggagaa aattacatac cactggaaga agccggagcg gttgcggcgg
    37021 taactggttg atattttcga aattcgccag cggcgaacat cggggaaacc agcctaaccc
    37081 gttgatattt tcgggaacct caaattgagg aggtcggaaa cataacccgt catattctgg
    37141 atgctgataa ttaccacgca gcccattaag gcctaacccg ttgattcttt cggaaacagc
    37201 cattggcgta atccggcgcg gtaacttgct gaacttaaag caaagcgcaa aattgcgctg
    37261 gctggtgggc atgatgccga tatggtgatc cccataacgg aaatatccac cagcacggca
    37321 acggcagaat agccacaaca cagaaaaacc acgaatatgg ggtttttgtt atggcatggg
    37381 catgatgacc gcccctgctc tgaatggtgg aaatcaccac gcagatcatc caccagcttc
    37441 gtttttataa attaaacatc cgggaaaatc cgaccctgaa ttgtgcattt tttcagcaaa
    37501 tcattttagt tatacgtttg gttatacggt ttaaaagttg aatcatatac attattttaa
    37561 atcatgaagt tacacgctca attaaactac tctcgtgcaa aaatcccaag aaaaaaccaa
    37621 cctttacggt tggttttttt atatctgcaa ttaatttgat aaacagaccg tgacacatca
    37681 cagcctgttt attttctgtt atcagaacgt ccagaccact cccgcctgag tattccacgg
    37741 cgattctacc cctgcgccat tgccatagct gactgacaaa tgaccgctta acgtcggggt
    37801 aaacgatgac cttatcccag cctgataaac gccaggggtt ccagccacat cattattaaa
    37861 tttgccatcg tcattaactt tcacatggtt agaatcggcg tattcctgac gtacggcggc
    37921 tttcagccag ggttccagcg tcgtaccatt ttgcaggtcc atgtgatagc ttaccgccgt
    37981 tcccgcttca gcgcgtaata tccgggtatt tcccacatca gcgcgcatgc cgtttgataa
    38041 cgtgtagtcc tgaccatctg tggtaaagcc ggtaaaggcc agatagggtc taacactcca
    38101 caatccgtca acccaacgga acccgctctc aacatgagca cccgcgccgt tactattgta
    38161 atcgccaaac gctgttgccc cattactcat cttgccatgg atggtgttgg caaaacggtc
    38221 aactttcacc accccatcaa cataggcacc gttctgatgc tcccaaccgg cataagcccc
    38281 cagggtatag ctatcgatat taccttttcc gccgcgatca aaaccaatat cagaatgaga
    38341 gtaaccaaag aacaagccgc gaattgtact gctttcttca cgggagaaac ggctatcgat
    38401 accgagcgtc aggcccgtca atgtttgctc aaaaccagct cccgcatcag tggtcacgtt
    38461 gttgcgggtg ttaattgccg aactccacat cgccgtatcg taactaacgc tttttacgct
    38521 accaagacgc tcacgcacgg tgtccagttc tgcatcaaat accagcggtt gtgcggccgc
    38581 catattcagc acatcagtgg ttgaaggggt aatttgcgcg cgattctctg ccaaactcca
    38641 gctatggttg ccattatcca gcaaggtata ttcatacgta ccgatatcaa caacgcctcc
    38701 ggcattgccc aacgtaaatg cagcatcacc gccgcccgtt gttaccagtg taaggctatc
    38761 tcctgctgcc gggctggcac cggtgtccgt cacgaatatt ttgaaatcac ctgttgcctg
    38821 accggtgacg ttgagctgat cgctctgatg attagccata tcggtacgca tataaaaatt
    38881 gccgtttccc gacagggtat tggtggtcaa cgtaatgaaa ttttccgcac ttgccgtcac
    38941 tgatgaacga gtcactggct catccatcat aatcacattt gcgtcagaca tcgcaaggtt
    39001 ggctaagttg aggagggtgt tttggttcgt cgtagtgata tcggcaatga tggcccatac
    39061 gctattttcg agcgaaacgt ccgctttgct ggtatccacg gcaccatgcg ttctaaaaga
    39121 agcgccctca gaaaccacta ccttacccac caacatagcg ggtgcataat ttatttgcgg
    39181 cttcatgacc tcaagaatgc catcattccc tctgactgac acgttaacca ttgtgccagc
    39241 ccagacatta gcgcggccat tgttgataac catattttca gagatggctt tagaggaata
    39301 cgtgatactt ccattctggt ctgatcgacc gagatcatat accgcatttg cctgcacttt
    39361 cgtaccagta tcctttccca gcgattgcat agtggcatgc tcatcaagga tagtatcaat
    39421 ggcctgcgtg tcctccataa caatgagccc ggaaccatca tccagttcat aattttttga
    39481 cacaccatct tttatactaa attggccttt actgtttgta ccactcactt ccagcgcatt
    39541 cgttgagaca attaattttc cgcctgcttt tttatcaacg ccagttgcag tcccgccatc
    39601 cataacctcc agtaagccgc cactatctac agtagtatta taagcgaagt cattttcttc
    39661 aactcgcaaa cttccgccgt tttccaacag catattgtta gcaataccat tcttgatttc
    39721 gaactgaccg agacggtttg ttccgaatgc ctccatgacc cgcgtgcttg ctttaatagc
    39781 accgcctgct ttctgatcta ccgcaatcgc taatcccccc tctttaacct ccagtaatcc
    39841 tcccttttcg atagcggcaa ggacggtaat cccatctttt tcaacgatta agcgcccgcc
    39901 tttaatcgtg ggatcattcg ctgcgccata aacctgtagc acaccattat tgagggtagt
    39961 ctggtttgcc gcgccaccca tttgaacaat ttgataaccg tcatttaata ctgagtattc
    40021 tgcggtacca ttcatttcaa tgtactgata ccctccgt
//
LOCUS       DAJTMC010000015        39753 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.015, whole genome shotgun sequence.
ACCESSION   DAJTMC010000015 DAJTMC010000000
VERSION     DAJTMC010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39753
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.015"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            4..435
                     /locus_tag="OO130_000711"
     CDS             4..435
                     /locus_tag="OO130_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HCR5364228.1"
                     /translation="MALNLASGEGNFFIRPGGVFYVAGDKVGIIRLDAFKTSKEIQFA
                     VQSGPMLLENGVINPRIHPNVASRKIRNGVGINKQGNAVFLLSQQATNFYDFACYAKA
                     KLNVEQLLYLDGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            518..1420
                     /locus_tag="OO130_000712"
     CDS             518..1420
                     /locus_tag="OO130_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HCR5364229.1"
                     /translation="MKKTLATLFLLTCLGSSSAYADNALILQTDFSLKDGAVSAMKGV
                     AFGVDHNLKIFDLTHEIPPYNIWEGAYRLYQTASYWPQGSVFVSVVDPGVGTDRKSVV
                     LKTKSGQYFVSPDNGTLTLVAQTLGIDSVREIDEKANRLKGSEKSYTFHGRDVYAYTG
                     ARLASGAITFEQVGPELPAKVVELSYQKAKATKGEVKGNIPILDIQYGNVWSNISDEL
                     LNQAEIKLNDTLCVTISEGSQQKYAGKMPYVASFGDVPEGQPMVYLNSLLNVSVALNM
                     DNFAQKHQVASGADWNIDVKKCAK"
     gene            complement(1465..3627)
                     /gene="uvrD"
                     /locus_tag="OO130_000713"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(1465..3627)
                     /gene="uvrD"
                     /locus_tag="OO130_000713"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HCR5364230.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCLPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTMHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLETV"
     gene            complement(3711..4427)
                     /gene="yigB"
                     /locus_tag="OO130_000714"
     CDS             complement(3711..4427)
                     /gene="yigB"
                     /locus_tag="OO130_000714"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HCR5364231.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(4427..5323)
                     /gene="xerC"
                     /locus_tag="OO130_000715"
     CDS             complement(4427..5323)
                     /gene="xerC"
                     /locus_tag="OO130_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HCR5364232.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDVTMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(5320..6027)
                     /locus_tag="OO130_000716"
     CDS             complement(5320..6027)
                     /locus_tag="OO130_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HCR5364233.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(6024..6848)
                     /gene="dapF"
                     /locus_tag="OO130_000717"
     CDS             complement(6024..6848)
                     /gene="dapF"
                     /locus_tag="OO130_000717"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HCR5364234.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(6893..7087)
                     /locus_tag="OO130_000718"
     CDS             complement(6893..7087)
                     /locus_tag="OO130_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004987677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5364235.1"
                     /translation="MKNVFKVLTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGMVHPR"
     gene            complement(7274..7570)
                     /locus_tag="OO130_000719"
     CDS             complement(7274..7570)
                     /locus_tag="OO130_000719"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5364236.1"
                     /translation="MKFTVVHCCILFLSDGGSIAAWGQGFKVCTSAAAFRHDSSVTGI
                     MMSLCGERFVSSLGRSYTTPFPVSGKHYDQVYRASILTLTELKKPTNKPHCDSA"
     gene            7550..7870
                     /gene="cyaY"
                     /locus_tag="OO130_000720"
     CDS             7550..7870
                     /gene="cyaY"
                     /locus_tag="OO130_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HCR5364237.1"
                     /translation="MNDSEFHRLADQLWLTIEEHLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(7910..10456)
                     /gene="cyaA"
                     /locus_tag="OO130_000721"
     CDS             complement(7910..10456)
                     /gene="cyaA"
                     /locus_tag="OO130_000721"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HCR5364238.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLCGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDHDTPLLQQYFS"
     gene            10843..11784
                     /gene="hemC"
                     /locus_tag="OO130_000722"
     CDS             10843..11784
                     /gene="hemC"
                     /locus_tag="OO130_000722"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HCR5364239.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDARTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            11781..12521
                     /gene="hemD"
                     /locus_tag="OO130_000723"
     CDS             11781..12521
                     /gene="hemD"
                     /locus_tag="OO130_000723"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HCR5364240.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPDYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLRCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            12543..13760
                     /gene="hemX"
                     /locus_tag="OO130_000724"
     CDS             12543..13760
                     /gene="hemX"
                     /locus_tag="OO130_000724"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCR5364241.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPAPA
                     PQGDTPAAAPQGE"
     gene            13763..14959
                     /gene="hemY"
                     /locus_tag="OO130_000725"
     CDS             13763..14959
                     /gene="hemY"
                     /locus_tag="OO130_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HCR5364242.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            15638..15799
                     /locus_tag="OO130_000726"
     CDS             15638..15799
                     /locus_tag="OO130_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5364243.1"
                     /translation="MEFSFSPKLLVVAVAAALPLMASAADTPSTATARKGFAGYDHPN
                     QYPQNAAIY"
     gene            complement(15883..17118)
                     /locus_tag="OO130_000727"
     CDS             complement(15883..17118)
                     /locus_tag="OO130_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HCR5364244.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYVRHYIAASEPQNEVAFTWQGGEPTLLGLDFYRRAVKLQAKYGAGRKISN
                     SFQTNGVLLDDKWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAASDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLLVVNK"
     gene            complement(17265..17341)
                     /locus_tag="OO130_000728"
     tRNA            complement(17265..17341)
                     /locus_tag="OO130_000728"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17305..17307),aa:Pro,seq:tgg)
     gene            complement(17384..17470)
                     /locus_tag="OO130_000729"
     tRNA            complement(17384..17470)
                     /locus_tag="OO130_000729"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17434..17436),aa:Leu,seq:cag)
     gene            complement(17491..17566)
                     /locus_tag="OO130_000730"
     tRNA            complement(17491..17566)
                     /locus_tag="OO130_000730"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17531..17533),aa:His,seq:gtg)
     gene            complement(17625..17701)
                     /locus_tag="OO130_000731"
     tRNA            complement(17625..17701)
                     /locus_tag="OO130_000731"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17665..17667),aa:Arg,seq:ccg)
     gene            complement(17804..19189)
                     /locus_tag="OO130_000732"
     CDS             complement(17804..19189)
                     /locus_tag="OO130_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5364245.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAVGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNESGSSGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(19380..20120)
                     /gene="rffM"
                     /locus_tag="OO130_000733"
     CDS             complement(19380..20120)
                     /gene="rffM"
                     /locus_tag="OO130_000733"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HCR5364246.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(20123..21475)
                     /gene="wzyE"
                     /locus_tag="OO130_000734"
     CDS             complement(20123..21475)
                     /gene="wzyE"
                     /locus_tag="OO130_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HCR5364247.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTREVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNREPNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(21472..22551)
                     /gene="rffT"
                     /locus_tag="OO130_000735"
                     /gene_synonym="wecF"
     CDS             complement(21472..22551)
                     /gene="rffT"
                     /locus_tag="OO130_000735"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HCR5364248.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNDHIAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRVLAIAAGEVA"
     gene            complement(22548..23798)
                     /gene="wzxE"
                     /locus_tag="OO130_000736"
     CDS             complement(22548..23798)
                     /gene="wzxE"
                     /locus_tag="OO130_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HCR5364249.1"
                     /translation="MSLAKASLWTAASTQVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(23800..24930)
                     /gene="rffA"
                     /locus_tag="OO130_000737"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(23800..24930)
                     /gene="rffA"
                     /locus_tag="OO130_000737"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HCR5364250.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGEHFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(24935..25609)
                     /gene="rffC"
                     /locus_tag="OO130_000738"
                     /gene_synonym="wecD"
     CDS             complement(24935..25609)
                     /gene="rffC"
                     /locus_tag="OO130_000738"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HCR5364251.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTPDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(25587..26468)
                     /gene="rfbA"
                     /locus_tag="OO130_000739"
     CDS             complement(25587..26468)
                     /gene="rfbA"
                     /locus_tag="OO130_000739"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCR5364252.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLVQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDQERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(26487..27554)
                     /gene="rffG"
                     /locus_tag="OO130_000740"
     CDS             complement(26487..27554)
                     /gene="rffG"
                     /locus_tag="OO130_000740"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCR5364253.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSDRFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVVHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(27554..28816)
                     /gene="wecC"
                     /locus_tag="OO130_000741"
     CDS             complement(27554..28816)
                     /gene="wecC"
                     /locus_tag="OO130_000741"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HCR5364254.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSTRASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(28813..29943)
                     /gene="wecB"
                     /locus_tag="OO130_000742"
     CDS             complement(28813..29943)
                     /gene="wecB"
                     /locus_tag="OO130_000742"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HCR5364255.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKAPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELVANYPFIDPNKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQTMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(29999..31045)
                     /gene="wzzE"
                     /locus_tag="OO130_000743"
     CDS             complement(29999..31045)
                     /gene="wzzE"
                     /locus_tag="OO130_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HCR5364256.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(31057..32160)
                     /gene="wecA"
                     /locus_tag="OO130_000744"
     CDS             complement(31057..32160)
                     /gene="wecA"
                     /locus_tag="OO130_000744"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HCR5364257.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(32400..33659)
                     /gene="rho"
                     /locus_tag="OO130_000745"
     CDS             complement(32400..33659)
                     /gene="rho"
                     /locus_tag="OO130_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HCR5364258.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(33744..33845)
                     /gene="rhoL"
                     /locus_tag="OO130_000746"
     CDS             complement(33744..33845)
                     /gene="rhoL"
                     /locus_tag="OO130_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HCR5364259.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(33986..34315)
                     /gene="trxA"
                     /locus_tag="OO130_000747"
     CDS             complement(33986..34315)
                     /gene="trxA"
                     /locus_tag="OO130_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HCR5364260.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            34446..35711
                     /gene="rhlB"
                     /locus_tag="OO130_000748"
     CDS             34446..35711
                     /gene="rhlB"
                     /locus_tag="OO130_000748"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HCR5364261.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEILEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            35845..37329
                     /gene="gppA"
                     /locus_tag="OO130_000749"
     CDS             35845..37329
                     /gene="gppA"
                     /locus_tag="OO130_000749"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HCR5364262.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVEKEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQESQWQSYVHWPLEVH"
     gene            complement(37376..39397)
                     /gene="rep"
                     /locus_tag="OO130_000750"
     CDS             complement(37376..39397)
                     /gene="rep"
                     /locus_tag="OO130_000750"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HCR5364263.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKYYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
BASE COUNT         9864 a        10800 c        10052 g         9037 t
ORIGIN      
        1 aaggtggcgt taaatctcgc ttcaggtgaa gggaatttct ttatccgtcc tggcggcgtg
       61 ttttatgttg cgggagataa agtcggcatc attcgtctgg atgcctttaa aaccagtaaa
      121 gagattcagt ttgcggtgca gtcagggcca atgttattag aaaacggcgt aattaatcca
      181 cgcattcacc cgaatgttgc ctcgcgtaaa attcgtaacg gtgtagggat taataaacaa
      241 gggaacgccg tgtttttgtt gagccagcag gcgacaaatt tttatgattt tgcctgttat
      301 gccaaagcga aactgaacgt tgagcagttg ctttatcttg atggcacaat ttcacatatg
      361 tatatgaaag gcggggcaat cccgtggcaa cgctatcctt ttgtcaccat gatttccgtc
      421 gagcggaaag gttaagcatt gtacactcga ggaggccaga caaactggga ctggctacac
      481 agctggaaaa tcatcagata aatgaaggga aacaaaaatg aaaaaaacgc tcgcaacgtt
      541 atttttatta acgtgtctgg gatcatcatc cgcctatgcg gataacgctt tgattttaca
      601 aactgatttt agcctgaaag atggtgccgt ctcggcgatg aaaggggtcg cattcggcgt
      661 tgaccataat cttaaaatct ttgatttaac acacgaaatt ccaccgtata acatctggga
      721 aggcgcttat cgcctgtatc agactgccag ttactggccg caaggttcgg tgtttgttag
      781 cgtggtcgat ccgggcgtag gcactgaccg taaatcggta gtgctgaaaa ccaaaagcgg
      841 tcaatacttc gtttcaccag ataacggtac gctcactttg gtggcacaaa ctctgggtat
      901 tgatagcgtc cgggaaatcg acgaaaaagc taaccgcctg aaaggttctg aaaagtcata
      961 taccttccac ggtcgtgacg tgtatgccta caccggtgca cgtctggctt ccggggcgat
     1021 tacgttcgaa caggtggggc cagagcttcc agcaaaagtt gtcgaacttt cttatcagaa
     1081 agcaaaagca acaaaaggtg aagtgaaggg caatatcccg attctcgata ttcagtacgg
     1141 caacgtctgg agcaatatca gcgacgagtt gctgaatcag gcagaaatca aactcaacga
     1201 cacgttgtgt gtgaccattt ccgaaggttc ccagcaaaaa tacgcaggga aaatgccgta
     1261 tgtcgcaagc ttcggcgatg tgccggaagg ccagccgatg gtgtatctga acagcttatt
     1321 gaatgtttcc gtggcgctga atatggataa tttcgcgcag aaacatcagg ttgcgtctgg
     1381 tgctgactgg aatattgatg tgaagaagtg cgctaagtaa ggtgctgatg cggaatgcgg
     1441 tgtggcgcac cgcatcccgc aacgtcacac cgtctccaac cgggcataag ccgccaccag
     1501 ccatttaata ccctggccct ggaatgccac ctgcaaacgg ctatgttcac cgctgccttc
     1561 catattgacg atggtgcctt caccaaactt agcgtggcgt acacgctggc cgagcttgta
     1621 gccgctgtcg ttctcgacca tcggcgtacc catccgctga tggctgaccg ggcggcttac
     1681 cgtggcgcgc aggcgcacct cttccacaca ctcttccggc agctcgccaa taaagcgcga
     1741 cgggcgatgg taaacctctt tgccatacag acggcgagtt tccgcgtagg tcagcgtcag
     1801 tttctgcatc gcgcgggtta cgccaacgta ggccagacgg cgttcttctt ccagacgccc
     1861 gccttcatcc agcgacatct ggcttgggaa catgccctct tccataccga cgataaacac
     1921 ctgcgggaac tccagacctt tcgccgagtg catggtcatc agttgtaccg catcctgcca
     1981 ggtatccgcc tgcccttcgc ctgcttccag cgccgcatgg gataggaacg cctgcagcgg
     2041 cattaaatct tcgtcttctt cgttgtagct gaactggcgc gttgccgtca ccagttcctc
     2101 taagttttcg atacgcgtct gacctttttc gcccttctcc tgctcgtaca tggtacgcag
     2161 gccggagtct ttaattaccc ggtcagtctg tacatgcagc ggcatatcgg cagtttcctg
     2221 cgctaaggcg tcgatcagtt ccataaaccg ctgtaaggcg ctggcagcac gtccggcgag
     2281 ggctttttcc tgcaacagtt cacgacatgc ctgccagagt gttaactggc gatcgcgcga
     2341 tgtctgacgt accacgtcca gcgtccggtc accaataccc cgcgttggcg tattcaccac
     2401 acgctcaaag gccgcgtcgt cgttacggtt ggcaatcagg cgcagatacg agagcgcatc
     2461 tttgatttcc tggcgttcga agaagcgcat cccgccgtaa atacggtacg gcatactcgc
     2521 ctgcaacagt gcttcttcca gcacacgcga ctgggcgtta ctgcggtaga gaatggcgca
     2581 ctcggcaagc gccccgccgt tgtcctgcca ggttttgatg cggttaacca caaaacgcgc
     2641 ttcatcgagt tcgttaaaag cgcaatagag ggaaataggc tcaccgtccg cgccatcggt
     2701 ccacagtttt ttacccagac gcccgttatt gttttcaatc agggcgttag cggcgctcag
     2761 aatattgctg gtagagcggt agttttgctc cagacgaata gtttcggcac cggggaaatc
     2821 attaaggaaa cgctgaatat tctccacctg cgccccgcgc cagccgtaga ttgactggtc
     2881 gtcatcaccg acgatcatca ctttgccggt gtcgcccgcc agcaggcgga tccacgcgta
     2941 ctgaatgttg ttggtatcct ggaattcgtc caccaggata ttggtaaaac gttcgcggta
     3001 gtgttgcagg atatgcggct tgttaagcca caactcgtga gcgcgcagca gcagctcggc
     3061 gaagtccacc aggcccgcgc ggtcacacgc ttcctgatac gcctgataca ccttctgcca
     3121 ggtctgctcc accggattac cgtagctttg aatatgatgt ggacgcaggc cttcatcttt
     3181 ctggctgttg atgtaccaca ttgcctggcg cggcggccac tgcttctcgt cgaggttcat
     3241 cgccttgatt aggcgtttga gcaggcgtag ctggtcctcg ctgtcgagga tctgaaaatc
     3301 ctgcggcaga ttggcgtcca tatggtgcgc acgcagcaga cggtgcgcca gcccgtggaa
     3361 ggtgccgacc cacatgccgc cctggctggt gcccattagc tgcccgatac gatgacgcat
     3421 ctccgccgcc gctttgttgg taaacgtcac cgccataatc gagtatggca agcagttttc
     3481 cacgctcatc agccaggcga tacgatgcac cagtacgcgc gtcttaccac tgcccgcgcc
     3541 cgccagcacc agaaggttgc tgcgtggcgc ggccaccgct tcgcgctgtt tgtcattaag
     3601 gctgtcgagc aggtaagaaa cgtccattgg caccgccgcg taaaaatagg ttgggcggag
     3661 aagcgcaacg ccctccgcca aaaagctggg tatatataca gatttgctga ttatatcagc
     3721 gaggtcagag atgccaaccg ggaaatttcc agatgcggca gtaaacggct gtcccaggtt
     3781 tgcatcagat cgccattttc cggtctgatc caacaggcct gcattccgct gcgaattgcc
     3841 ccacccacgt cagtggtgag atcgtcccca acatgtaaga tctcgccgat cggcacgttg
     3901 agtttttccg cagccagaaa gtacatatcg ctgaacggtt ttgagcgccc gtgcgggcca
     3961 gcgcgcagca caaactcaaa ataatccccc aaaccaaaca gttccggctg ggcgttaccg
     4021 ttggtgatcg ccaccagcgg ccatttcttc gccagctgtt ttaaggtgtc gtgagtttgc
     4081 tgcgggacgt cgattcggct gcgccatttg gcaaagttga tcattgctgc gtgtgcgcct
     4141 gcactggctt cttcggcact cagcccggcg tcgagcatcg cttgttcaat cgaacgaaaa
     4201 cgccagcgcg tcacgtcgtg ataaatctcg ggttccgctt cccgtaccgc ctggcgcagg
     4261 cgttgcagat cttcattctg gaagctgcgc agcgccggat gataattttg cacaaaggta
     4321 agcgcctctc gctcggtgcg caaaatcacc ggacggttat cgtaaagggt atcatccagg
     4381 tcaaaggtga gcgccgagat gcgccccaaa ggccggtaaa aacgcattat ttcccccgtt
     4441 tggcgcgtgg atgcgccgca tcgtacaccg aggcaaggtg ttgaaaatca agatgagtat
     4501 agatttgcgt ggtggagagg ttggcatgac ccagtagctc ctgcacacca cgaagatcgc
     4561 cgctcgactc cagcatatgc gtagcgaacg agtgacgtaa tttatgtggg tgaacgtgat
     4621 tattcagccc ttgttttatg ccccattcgg caaagcgttt ctgcacatta cgcgcggaga
     4681 tacgcttgcc cagtttcgac agaaaaagcg cgtcgtcttc gctaccaaac aggtcgcgca
     4741 aatcaagcca gtgctcaatc cacgccacag cgttgcgacc aatcggcagg cggcgctctt
     4801 tgctgccttt ccccattacc cacacttcac cagactccag gtcgaggtgt ttgatatcca
     4861 gccccaccag ctcggaaaga cgcagaccag cgccgtacat cacttccagc attgcacggt
     4921 cgcgtacagc gaggggatcg ttgatatcaa tatccagcag ccgattcata tcgtcgacgt
     4981 cgatattttt cggcagatga cgcggcgctt tcggtgccga aacacctttc gccgggttag
     5041 ctttgagttc gttctggctg accagccagt caaaaaagct acgtagcgca gaaagccgta
     5101 acgccagact tgctgctccc agccctttac ggcgactgcg tacagcaaaa ttgcgcacca
     5161 tcgtcacatc acattgctgc cagctttgca ggccgttttc gctggcaaaa ttgatgatcg
     5221 cctcaagctg acgctggtag ttaagcaggg ttatcgggct aagctggcgc tccacgctca
     5281 gataacgtag gtagcgttct acatcggtgt gtaaatcggt catacgcgtt caatccaacg
     5341 ctccagaagc tccggcaaca tcagcgcaat ttcatgaagt aactgcgttc cttgcccttg
     5401 ttgatagtga ctggcatcgc gactggtaaa cagcacgaca cccaaatcag catcgcttcc
     5461 cagcatcgac atcgccaccg atcccaccgc tttcgcttcc ggtagcacca ccagcagctc
     5521 tggtccgtta agcggcccaa gatagtgctg ttcctgcccc aaacgctgaa tacgcagcgg
     5581 ttcgaaagac tgacggctta atgccagatg agtgtggttc gacggcgcac ctaagcgcca
     5641 gcgatccgga aacaggcgca gactcgcacc tgccaggccg agatcgcgcg cccagcggtg
     5701 aaagcgcatc agcatatcgt cgagactgct ggcggcggtg agactgcgct gcaggtagag
     5761 taggcgataa aacaggcctt cgttggcgat agcctgttcc atcaacagcg ccatgttctc
     5821 ttccagaaca tgaatatgat tacgtgcgcg ggccatgtgc cactcgacca acgaaacggt
     5881 gccgcgtacc ggatgtggca cacgtatcgc ttctactgcg cgcgcattac ggataaaaaa
     5941 ctcaggattt ttaatcagat aatcgacaac cgcccggtca tcaagctccg tgagtgtttc
     6001 ctgcagttct tcccctggtt gcttcataga tgaataaatc cgtcgtagac atgtaccgcc
     6061 gggccagtca tatataacgg gtgacccgga cctttccagg cgatatcaag acgaccgccg
     6121 gggagttcca cgcgtacttc ttcggccagc aaaccttgct gaatccctac tgcaaccgcc
     6181 gcacacgcgc cgctgccgca ggcctgggtt tctcctgccc cacgctcata aacgcgtaaa
     6241 cgaatatgct cgcgcttaac cacttgcata aaaccgatat tggcgcgctc cggaaaacgc
     6301 tcgtggcttt ccagaacagg accaagcgtt tctaccgccg cggtatcgac atcatcgacc
     6361 tgaatcacgc aatgcggatt tcccatcgac accacgccgc ataagattgt ctgctcggcg
     6421 gcgcgcataa tataggtctt ttccgctttg ttagcgcgaa acggcacagc ggaaggttcg
     6481 aagttgggtt cgcccatatt tacgcggacc agatcatcat cggtgacggt cagaaccatc
     6541 cgcccgttgg cggtgctgac gcggatatcg cgcttattgg tcagcccttt caggcgcaca
     6601 aaacgggcaa agcagcgcgc accgttgccg cactgcgcca cttcactgcc atcagcattg
     6661 aaaatgcgat agtgaaaatc cagttcagga tcatacggcg gctcaaccac cagcagctgg
     6721 tcaaacccta cccccaggtg ccgatccgcc agccgacgaa tcagctccgg tgaaaaaaag
     6781 acattctgcg ttaccgcgtc gacgaccata aaatcgttgc caaggccatg cattttcgag
     6841 aactgcatca tttactccaa tcacgcgggt acagaaactg acttttagta attcacctgg
     6901 gatggaccat cccagtggcg cgatcgtttt tatccggcac cgtggattgc gtttgcgtct
     6961 ctaccggttt ggtcggcggc ggtgcatttt tatctgcagg cgggaaatag agcggacctt
     7021 tcagaccgca gcccgtcagg ctgaagagag taagtaatac agtgagtacc ttaaacacgt
     7081 ttttcattat ggattgcctg taaagttcat cgctttctgc tttctatcat cgcaggagaa
     7141 ggcgtaaaag caagcgttta gcgactcttt atgaagtctg aaaagtgagg aaatgctgcg
     7201 agtataacgc ttactcgtga ggatttatgt tctgctggca gtttcgtgat cggcacctcg
     7261 cgccaggatg gctttatgca ctatcgcaat gtggtttgtt tgttggcttt tttagttccg
     7321 tcagcgtgag gatgctcgct cggtaaacct gatcataatg tttaccagaa acagggaaag
     7381 gcgtggtgta actgcgccct aatgaagaga caaatctttc gccacacaga gacatcatta
     7441 ttccggtgac ggatgaatca tgccggaacg cagcagcaga ggtgcaaact ttgaagccct
     7501 gaccccatgc cgctatactg ccgccatcac ttaaaaacag gatacaacaa tgaacgacag
     7561 tgaatttcat cgcctggctg atcaactgtg gctgaccatt gaagagcatc tggacgactg
     7621 ggatggcgac agcgatatcg actgcgaaat caacggcggc gtgctgacca ttacctttga
     7681 aaacggcagc aaaatcatta tcaaccgcca ggaaccgctg caccaggtat ggctggcaac
     7741 caaacaaggc ggctaccatt ttgatctgaa aggcgatgag tggatttgcg atcgcagcgg
     7801 cgaaaccttc tgggatttgc tggaacaggc ggcgacgcag caggcgggtg aaacagtcag
     7861 tttccgctaa gattgcatgc cggataagcc tcgctttccg gcacgttcat cacgaaaaat
     7921 attgctgtaa tagcggcgta tcgtgatcct gattggcagg cggcatgtta ccgatagatt
     7981 ttgtgcggaa cggaatcacc tgttcacgac catcaacctt cacaatctga tagaactgcg
     8041 gcaggttgaa gttgatgaag cttgagccgt aggtgaagcg gtcgtgcgat gacgagtaga
     8101 agcgactgac gtcacgcacc agttcctctt tgctgccttc gcagtggtga tacacctcaa
     8161 cccggttgct ttcgtcaaga atgtagatat taaagccatt ctcgtcttgc gtttcttcga
     8221 agaagaactg gatgatccct tcactagcaa agccgtccac caccgccggt aatttgacgt
     8281 gattggtttc aacctgcact gacaggccgt gcagtttgtt atgcgaaata gcgccataaa
     8341 actcgatggc gttctccagc ttctgtaccg atacattcag gcgttcgaag aacaagcccc
     8401 aggtttgacc agaaacgcgc agcgccttga aacgcccggt ttcctggcgg gtgctggaaa
     8461 ggcgcagttc aatacactca gaaaccagtt gctgcacgcg agtacggatt aacccgcaca
     8521 gatgctggct ataacagaag acttccacgc tatctggcgg tgcggcgtcc tgatgcattt
     8581 tgccgagaat agttttcagg gcttcgatca tcgattgctc gccgttgaag tgcagcgtac
     8641 gcacttcgtt ccacgagttg cggtacagca ggtcaacgct acctaccagg caattttgat
     8701 tctcgccaaa gctgaagaca tccagcttac ggaaatcaaa atgcaccacc tgattgcgga
     8761 acgccgctgt cgggtcatat tccaggttaa cgataatcgc cagatggcgg atctcacacg
     8821 ggctgtagag cgccttcggt gtcggtgcag gtaagcgcag cgggaaatgg tgcgacacgt
     8881 cggcgaccat ctcctgcaac ttaggcaaat cgacaatgcc gttaccttta atatacaaac
     8941 gggtgcgcga ggtcagcagg ccgttaaacc atgcccacgc caccagtttg ttcaggtaac
     9001 ggttatattc cagcggctga tggctgatga tcgactcaat atttggcgcg cggttataca
     9061 gataccaacc tgaacggtta gcacggcccg gcggcacata aataaaggtc agattcggtt
     9121 ccgagagatc gggtgaaatc tgcgggttta ccagcgtcac tttacctggt aatgcttcaa
     9181 acgcggcata cagcttacgg gtcagcacgc cgatatcctg cggactggcg gagacgctaa
     9241 ggttattgcg acgcgcaaag cggatcagat tacggtagct ctgcatcatc gcgtcgagca
     9301 actcgttgtg cgcctcacgc acctgatcaa tcttccagtt agcgcggtta tcgagcattg
     9361 ccagacgagc ttcgtcccaa ccccactcgc tcactaactg gctcaacact gcgcgacgcc
     9421 agcctacgca ggcgcgttca cggctgagct tttcgcacac ttttaaatag aagcagcgac
     9481 gtactaaatc cagacgggta aaatcttcaa tcgccgtcag gtattcagta acacgctcca
     9541 gcatcatgca gtatggatcg agaccaaacg atacaatctc accgtcgtgc aaacgctgtt
     9601 tgatatcttt cgccagcaga cgtgggttcg ggtattccca ggaataggct tccagcagca
     9661 gtgttttcag taccgctttg tatggggaat cgatactctt gtagagctgc caaaggctgg
     9721 caccaaagta ctcttcagca gaaagcgagc ttaagccacc gagatccagc cattcatttg
     9781 gcgtcagcac gccctgcgcg taaagcgtca tcacatagtc gtcgtaatgc tcttcttcgt
     9841 cgcacggcac catattccac agaatacgct taccggcgag acgcacggcg gtacgataaa
     9901 attcgtcaag cagcagtata tgctgggtgg agccacaatc ttcgcccccc aagctgccgc
     9961 tttcattatg acggaagcgg ttttcatcaa tcaggaagaa gctgacttcc acacccagcg
    10021 aggcggccca gctttccagc agactacatt tacgttgtag taactggcgc tcttcgctat
    10081 cgagccagga ttgatgacag acccagatat ccaggtcaga ggaacaactt tgccctacgg
    10141 acgaggtgct gcccatggtg tatacaccag taattggaag ctcacctttc ggtggatcct
    10201 gtactgacat tccacgatac agttcaagct cgttcaggta gtggcgttga gtttcatcag
    10261 gcgtgtaaag gcaaatgcct ttgggaacgt taccatcaag gtaacccggc attagcggat
    10321 ggtgatagtg caacaatgtc ggcagtagac tgtagacctg ttggaatgca ggccccatag
    10381 cagcaagcgc gcgatccaca cgcaattgat ttatggcatc cagtctctgt ttcagagtct
    10441 caatatagag gtacaagacg tatcgcctga tttgctaccc gtcatgactg tgattccgcc
    10501 aacatcaacg gtaacacgcg gcattcggga tatttcgtat gtcaaaggta accgttacca
    10561 cttttcgcgc ctggtttttt tagtttcacg acgaaaaaat ggtctaaaac gtgatcaatt
    10621 taacaccttg ctgattgacc gtaaagaaag atgcgctaca tacaagtgta gcaccgttta
    10681 ttctctgtaa attccttatt acgacggcgt gaaacgcctg tcaggatcca ctgccagacc
    10741 tcattttacg gtttgcgcag gcgtctacgt ttcaccacaa cactgacatc actctggcaa
    10801 ggatgttagg atggaccacg gatgataatg acggtaacaa gcatgttaga caatgtttta
    10861 agaattgcca cacgccaaag cccacttgca ctctggcagg cacactatgt caaagacaag
    10921 ttgatggcga gccatccggg cctggtcgtt gaactggtac cgatggtgac gcgcggcgat
    10981 gtgattcttg atacgccgct ggcgaaagta ggcggaaaag gcttatttgt aaaagagctg
    11041 gaagtcgcgc tcctcgaaaa tcgcgccgat atcgccgtac attcaatgaa agatgtgccg
    11101 gttgaattcc cgcaaggtct gggactggtc actatttgtg agcgtgaaga tcctcgcgat
    11161 gcctttgtgt ccaataacta tgacagtctg gatgcgttac cagcaggcag tatcgtcggg
    11221 acgtccagtt tacgtcgcca gtgccaactg gctgaacgcc gcccggatct gattatccgc
    11281 tccctgcgcg gcaacgtcgg cactcgcctg agcaaactag ataacggcga atacgatgcc
    11341 atcattcttg ccgtagccgg actaaaacgt ttaggtctgg agtcccgcat tcgcgccgcg
    11401 ttaccacccg agatttctct tccagcggta ggacaaggtg cggtcggtat tgaatgccgc
    11461 cttgatgatg cacgcactcg cgagctgctt gccgcgctga atcaccacga aactgcactg
    11521 cgcgttaccg cagaacgcgc catgaatacc cgtctcgaag gcggatgtca ggtgccaatt
    11581 ggtagctacg ccgagcttat tgatggtgaa atctggctgc gtgcgctggt cggcgcgccg
    11641 gacggttcgc agattattcg cggtgaacgc cgcggtgcgc cgcaagatgc cgaacaaatg
    11701 gggatttcgc tggcagaaga gctactgaat aacggcgcgc gcgagatcct cgctgaagtc
    11761 tataacggag acgccccggc atgagtatcc tggtcacccg cccgtctccc gctggagaag
    11821 agttagtgag ccgtctgcgc acactggggc aggtggcctg gcattttccg ctgattgagt
    11881 tttctccggg tcgacaatta ccgcaacttg ctgatcaact ggcggcgctg ggggagagcg
    11941 atctgttgtt tgccctctcg caacacgcgg ttgcttttgc ccaatcacag ctgcatcagc
    12001 aagatcgtaa atggccccga ctacctgatt atttcgccat tggacgcacc accgcactgg
    12061 cgctacatac cgtaagcgga cagaagattc tctacccgca ggatcgggaa atcagcgaag
    12121 tcttgctaca attacctgaa ttacaaaata ttgcgggcaa acgtgcgctg atattacgtg
    12181 gcaatggcgg tcgtgagcta attggggata ccctgacagc gcgcggtgct gaggtcactt
    12241 tttgtgaatg ttatcaacga tgcgcaatcc attacgatgg tgcagaagaa gcgatgcgct
    12301 ggcaatcccg cgaggtgacg atggtcgttg ttaccagcgg tgaaatgttg cagcaactct
    12361 ggtcgctgat cccacaatgg tatcgtgagc actggttact acgctgtcga ctattggtcg
    12421 tcagtgagcg tttggcgaaa ctcgcccggg aactgggctg gcaagacatt aaggtcgccg
    12481 ataacgctga caacgatgcg cttttacggg cattacaata actctcataa caggaagcca
    12541 taatgacgga acaagaaaaa acctccgccg tggttgaaga gaccagggag gccgtggaca
    12601 ccacgtcaca acctgtcgca acagaaaaaa agagtaagaa caataccgca ttgattctca
    12661 gcgcggtggc tatcgctatt gctctggcgg cgggcgtcgg tttgtatggc tggggtaaac
    12721 aacaggccgt caatcagacc gccaccagcg atgccctggc taaccaactg acggcattgc
    12781 aaaaagccca ggagagccaa aaagccgagc tggaaggcat tattaagcaa caagctgcac
    12841 agcttaagca ggcgaatcgt cagcaagaaa cgctggcaaa acagttggat gaagtccaac
    12901 aaaaggtcgc caccatttcc ggcagcgatg ctaaaacctg gctgctggct caggccgatt
    12961 ttctggtgaa actcgccgga cggaagctgt ggagcgatca ggacgtcacg accgctgcag
    13021 cgttgctgaa aagtgcagat gccagcctgg cggatatgaa tgacccgagt ctgattaccg
    13081 ttcgtcgggc aattaccgat gatatcgcca gcctttctgc agtttcgcag gtggattatg
    13141 acggcatcat ccttaagctt aatcagcttt caaatcaggt agataacctg cgtctggccg
    13201 ataatgacag cgatggttcg ccgatggatt ccgatggtga agagctttcc agttccatca
    13261 gcgaatggcg tatcaatctg caaaaaagct ggcagaactt tatggacaac ttcattacga
    13321 ttcgccgtcg tgatgacacc gccgtaccgc tgttagcgcc aaatcaggat atctatctgc
    13381 gcgaaaatat tcgctctcgc ctgctggtcg cagcacaagc tgtaccgcgt caccaggaag
    13441 agacttatcg ccaggcgctg gagaacgtct ccacctgggt acgtgcttac tacgatactg
    13501 atgatgccac caccaaagcg ttcctcgacg aggtggacca gttaagccag caaaatatct
    13561 cgatggatct tccggaaacc ctgcaaagcc aggcgatgct ggaaaaactg atgcagactc
    13621 gcgtgcgtaa cctgctggca caaccggcag cagggacaac ggaagctaaa cctgcacctg
    13681 cacctgcacc tgcacctgca cctgcacctg cacctgcacc gcaaggtgat actccggcag
    13741 ccgcgccgca aggagaataa ccatgctaaa agtgttatta ctctttgttt tgctgattgc
    13801 ggggatagtg gttggcccga tgattgccgg tcatcagggt tatgtgctga tccagaccga
    13861 caactacaat atcgaaacca gcgtcacggg cctggcgatc atattgattc tggcgatggt
    13921 agtgctgttt gccattgagt ggctactgcg gcggatcttc cgcactggcg cgcacacccg
    13981 tgggtggttt gtcgggcgta agcgtcgccg tgcacgtaag cagaccgaac aggcgctgct
    14041 gaaactggcg gaaggcgatt atcagcaagt tgaaaagctg atggcgaaaa atgccgatca
    14101 cgctgaacaa ccggtggtga actatctact ggctgccgaa gccgcgcaac aacgtggtga
    14161 tgaagcgcgc gccaaccaac atctggaacg cgcagcggag ctggccggca atgacaccat
    14221 tccggtagaa atcacccgtg tacgtctgca actggcccgt aatgaaaacc atgctgcacg
    14281 ccacggcgtg gataagctgc tggaagttac gccacgccat ccggaagtat tacgtctggc
    14341 ggaacaggcg tatatccgca caggtgcatg gagttctctg ctggatatca tcccatcaat
    14401 ggcgaaagcc catgttggtg atgaagaaca tcgtgcaatg ctggaacaac aggcatggat
    14461 tggcctgatg gatcaggcgc gtgccgataa cggtagtgaa ggtttgcgta actggtggaa
    14521 aaaccaaagc cggaaaacgc gtcatcaagt agcgttgcag gtggcaatgg cggaacatct
    14581 tattgaatgt gacgatcatg atactgccca gcaaattatc atcgatggcc tgaaacgcca
    14641 gtacgacgat cgcctactgc tgccgattcc tcgactgaaa accaacaatc cggaacagct
    14701 ggaaaaagtg ctgcgccagc agatcaaaaa cgtcggcgat cgcccgctgt tgtggagcac
    14761 actgggccag tcactgatga agcacggaga atggcaggaa gcatcgctcg ccttccgcgc
    14821 agcgctgaaa caacgtccgg acgcctacga ttacgcatgg cttgccgacg cgctggacag
    14881 actgcacaag ccggaagaag ctgcagcgat gcgtcgcgac ggtttgatgt taacgttgca
    14941 gaacaaccca ccacagtagt tccttctcac ccggaggcaa gcacctccgg gacctttctg
    15001 atgcataaaa aaacgcctgc tcttattacg gagcaggcgt taaaacaggt ctgtatgaca
    15061 acaagtgggt gcttcactca acgttgtgtc catggtgtct gatgaggcat aagcgacatc
    15121 tgtcagtgga cgataagcac cgtaaacggc tctgcgtcat tccggagttt atgaggcact
    15181 aaggcgaaca taagagatgg aatgagcatc tactcgttta ttatgccaca gagaatcggg
    15241 aaataacatc ccttaacact tgttatgaga taattctgta atcctctttg cttcctgagt
    15301 aataacttcc tgagtgaata tttaacctga gcttgatcct acacatactt attatgaatg
    15361 ataaaattca ttcaattaat aacacatata ttaattgccg ttaaaactaa aaaaagcatc
    15421 aataatcaac gcgatataat aaacctgcct tacatatcaa ctgcgccaga ggtaggattg
    15481 aaaacgctct cctgattttc caattcattt tctggataaa taaataattt atttttgtca
    15541 ctattattta tgtaatcatc ctgtcaggga gagggatctc aattatcaat gcttaattac
    15601 gtcatcattt tgatgacatg aaaccggagc attttgcatg gaattttcgt tttcacccaa
    15661 acttcttgtt gttgctgtcg ccgccgctct tcctctcatg gccagcgcag cagatacccc
    15721 gtcaactgcc accgcacgca aaggctttgc cggatacgat cacccaaacc agtatccaca
    15781 gaacgctgca atttattgaa tttgcacgat catgtaggcc ggataaggcg tttacgccgc
    15841 atccggcaat caaccgcagg cggccacaaa atggccgcta ttttacttat tcaccaccag
    15901 caaatgtgcc tgcataatgt cactggccgg gcgaccgtgc gccagcaaat cagccattgc
    15961 tttaagatat ggcggtagat ggcggaaata acgctgatac ccggcacaca aataattcag
    16021 ccccggtttg ccgctggcat cgagcatgaa gcggtgtttc gggcagcctc cccagcacgc
    16081 ttttaacacg ttacaactgc gacactgcgc cggtaattgc ttaaatttat cttcaccaaa
    16141 cacctgctgt tgcggggaat cgatcatttc tgcaattgtt tgctgatgca tattccccag
    16201 ccgatattgc ggataaacat agtgatcgca ggcgtaaacg tcgccgttgt gctcaacaat
    16261 caccgagcgc ccacaggttg gctgatgatg gcaaaccgca cccggcgcac cgacaaaatt
    16321 ggcaaacgcc cattcgatat tcatcacgaa aatcttgccg acgtcgcgtt tgatccagtg
    16381 gtcaaaaatc gccaccagaa attcaccaaa ttcatcgggg cgcacagacc attccgtcag
    16441 ttccccctga atatcaccag gcgcatgtag tttcagtcca tcgctggcag ctgtttcatc
    16501 agctaggcgc tcgaccaccg gaataaactg gatgaattcg actcccgcat cgcacaaaaa
    16561 atcataaacc tgcaacggtt gctgcgcgct ggtgcggttg acgcagacca gcacgttata
    16621 gtcgacatga tgtttttgca gcagcgtcag ggcacgcatc accagcttat gcgttggtct
    16681 gccacctttg gtcacgcgat attgattgtg gatctcagcc ggaccgtcca gcgataaccc
    16741 aacaagaaaa tgattttctg ccagaaatgc acaccattta tcatcgagca gcacgccgtt
    16801 agtctggaag ctgttactta tcttcctgcc agcaccgtat ttcgcctgta actttacggc
    16861 acggcggtaa aaatccagcc cgagtaacgt tggttcgccg ccctgccagg taaaagccac
    16921 ttcgttttgc ggttcgctgg cagcgatata gtgacggaca tacgcttcca gcgtgtcatc
    16981 gtccatatgc gtgactggct tttcgcggta aagggattgt ttttcgagat aaaaacagta
    17041 gtcacagttc agattacaat cggaaccact cggtttcgcc atcacatgaa aagcacgcgt
    17101 tggaacctgt tgcagcatgg tcactccttg cctgaagtca tccttcaata cgttaagggc
    17161 gaaatcatac cgtggggcaa tgagttatta cgggcgttta ttgcgaagag agggtgacga
    17221 aatgcccaga aaacaaaaaa ccccgccgaa gcggggttca aaattggtcg gcgagagagg
    17281 attcgaacct ccgacccact ggtcccaaac cagttgcgct accaagctgc gctactcgcc
    17341 gaaatactgc tttttgaatt tttagttcaa ttatttaaag tcgtggtgcg agggggggga
    17401 cttgaacccc cacgtccgta aggacactaa cacctgaagc tagcgcgtct accaattccg
    17461 ccaccttcgc attgtcacaa cttctaataa tggggtggct aatgggattc gaacccacga
    17521 caactggaat cacaatccag ggctctacca actgagctat agccaccact acaaatcttg
    17581 ttacgcggta ttactaccac cgcagctcaa gcgccgggac taaatggcgc gcccgacagg
    17641 attcgaacct gagacctctg cctccggagg gcagcgctct atccagctga gctacgggcg
    17701 cttagcgccg ttgcgggggt ggataatacg gacttcccac ccctctgtct agtccctttt
    17761 taaataaatt gcgcgtttgg ttacggtttg tgcgttttgc tgcttattcc tccagtttat
    17821 gcgctttccc gtggcgatta aggccaaaaa ctttataaat cgccgtcacc gccagcataa
    17881 agatgatgcc aacaaacagc gacatacgcg tatcttcatt aaagtacatg ccgatcaaaa
    17941 cgcaaatcag gaatgccatt gttacgtagt tggcccacgg gaacaggatt gagcggaacg
    18001 gatggctggc aatcgccgct ttatgtgcac gccgaaaacg cagctggctt atcaatatca
    18061 caaaccatgg caccatcccc ggaagcacgc tggcactgta gacgtagaca aacacacgct
    18121 gcggattggg aatgatgtag ttcaggcatg agccaattag cagaatagca atagatactg
    18181 ccacacccgc aactggtacg ccgtgacggg aaactttcgc catcgccgcc ggtaactgac
    18241 ggtttttcgc cagtgcgtag agcatacgtc cgcaactgta catgccgctg ttacagccag
    18301 agagcgcagc cgtcagcacc acaaagttga taatgcccgc cgctgcggta ataccgattt
    18361 tggcaaaagt cagtacgaac gggctgccgc tgctgccact ttcattccac gggaagatgg
    18421 tgacgataac gaaaatcgcg cctacgtaga aaatcaggat ccgccacagc actttgccta
    18481 cggcactgcg caacgtcacc tgcggattct tcgcttcacc ggcagtaatg ccaatcagct
    18541 ccacgccctg gtaggacgcc accacaatac acagcgcggt caggaaccct ttccagccac
    18601 ccgcaaagaa accgccatgc tctgtgagat tgctaaaacc aatcgactgc ccgccattgc
    18661 caaagccaaa gaaaatcacg cccaggccaa tgacaatcat cacgataatc gtggtgactt
    18721 tgatcatcgc gaaccagaac tcgatttcgc cgtacaaccg caccgccgcc agattcgcca
    18781 acgccaccag cgccactgcg atcaatgcgg gtatccactg cgccatctcc gggaaccaga
    18841 actggacata aacgccaacg gcggtgattt ctgagatccc caccgccatc cacataaacc
    18901 aataagacca ggcggtgaga taaccaaaga acgggctcat ataacgatgc gcataaacgg
    18961 cgaacgaacc ggtaaccggt tcgaggaaca acatttcgcc cattgaacgc atgatgaaaa
    19021 agacgaatag cccggcgatg atataggcca acaatacgga tggcccggcc catttcaagg
    19081 tactggcggc ccccataaac aggccgacgc caatggtgcc ccccagggcg atgagttcga
    19141 tatgtcgagc ttccagccca cgctgtagct ctggtttgtt atctgccata aatcctcgtg
    19201 ttgtgtttgc atgctttccg gtgttaccgg ttatcgttat gggtacatcg agtgttgcga
    19261 atgttttcgt aattcaggag aaatggcaaa taaagcatta aaaatttgaa tgctttgtgt
    19321 aataaaaaag cagacaggcg acggagtgac cactccgtcg ctttacaaag agaggaaaat
    19381 cagaggttgc cggtgtagtg ccagcgtaaa taacgcagca aacgaagctg acgcttaatg
    19441 cggctcggct gcgaaagcag gcggtagagc cactccagtc ccagcgtttg ccagattttc
    19501 ggtgcgcgtt ttacgtgacc ggtgaaaaca tcgtaagtcc cgccaacgcc catatacagc
    19561 gcatctggat gcaccagacg gcagtcgcgc atgaagatct cctgctttgg cgatcccatc
    19621 gcaacggtga cgatttgcgc tccgctggca tgaatgcgtt caaacagcgc ctgacgctgc
    19681 tcgggtttaa aataaccatc ctgactgcca acgatattca cattccactg gttgcgcagt
    19741 ttagcttcag tttgcgccag cacttcaggt ttaccgccca caagaaatac cggcgtccct
    19801 tctttgcctg cgcgcgccat cagctcttcc cagagatcgg caccggcaac gcgggaaacc
    19861 tgcgcctgcg ggtacttttt acgtactgaa cgtacaacgc tgatgccatc cgcatattta
    19921 aattcggcag cgttaattaa ctccctgacc tcggcgttat cttcaatggt cagcattttt
    19981 tcagcattaa tggcaaccag cgttccctgc ttaagctgcc cgtcagcaaa cagataatcg
    20041 agggcgtgct gcatatcacg ccaaccaatc aactgtaagc cacgcagcgt ataggtcggt
    20101 gccgtggtgt tgttattcat tgttatcctt caacctgcgt ccggagcgat gattttgtac
    20161 gtttatgaat gagtccggcg ctttcaaaaa gccagtacaa cagttttgcg atcatcagac
    20221 atgcgccgaa gaccacgata aaaaagacta cgcgtgagac aaacgaatcc agcccttcac
    20281 gtgccagcac gatcatattg aagatcgccc caaagcagaa actgtgcaat atcgcagcct
    20341 tatagcgatt aggctcgcgg ttgcccagct catacagcca gtcgaaccat ttgatgatca
    20401 gaccaaccac gatcgccccg agcgggatga acaacgcgcc gcccatcacc accagtgagc
    20461 ctataagcgt aggcgagatc gccagtccgg agtggttatt cagcacttcc ctggtaaagt
    20521 agttggctga gttcagcacc atactcgggc gacccggcca cagccaggaa gggataaaga
    20581 catagaaatc gcggacaatt ggagccaggc cctggaagtc gatgttgtcg tagttctgca
    20641 acagcaacgc cagattctcc cacggcgaga aggtgtcgcg agtgagatag agaaacgtat
    20701 agaacgcttc atcgccgctc acattcattc catagcgttt tagcgccagc cagaacatgc
    20761 ccacaatccc cagcacgccc gccgccgcca gcatccacaa cgaaatccag ccgcgaataa
    20821 tgccaataaa caggaagata gcgaatgcga tgatgatatt ggcgcgagtg ccgccgacaa
    20881 tcatataagt cagcaagcca aaggcgaccg tgctgacgag gaaaaacagc cacgctttgc
    20941 tatcctggcg cagaaagtag accaccagca tcgccgggat gaaaaagtaa aagaagcgtt
    21001 ttaacgccac gccggagact tcactggaga agatctgact gtaggagtta agccggaaca
    21061 gcaaaaagcc gttgtgcatg aagaagatgc cgacgcttac cagcgcgatg cccatcagga
    21121 tcacccacgt aagattggtc tccacgcggt tcatggtaaa cagcggacgg cgcggtacat
    21181 cagcaacgcg tttgcgtagg cgggttttgt aggtgacata gtaaaccgcg tagaagcagc
    21241 ccgcagaaag caacgcctgc aacaagattt ctggcggcgc gacaccaaca tcaaagcgaa
    21301 ataccagcac gctggtcagc gggaagccga agaaaaaggt gagcaaaaac agcaatgaaa
    21361 agaagacatt gaagttaaag cgcacacggc gaaactcaaa ccaagtcagc gtggcaataa
    21421 acagcgtgca gagcagccag accacaaaca ggccactgaa ttgcagcaga ctcatgcgac
    21481 ctccccggcg gcaatcgcca acacccgctg ccagccttgt agatagttag ggctaaagaa
    21541 ggcaatggtg tttttatcca ccgacgccaa ctggcgctgc gcttcacgca caatatcctc
    21601 gttgagatcg tcggtagtaa acagcaccgg caaatgctgt tccgtcatat cctgccagaa
    21661 cggattttcc cggttaagca cacaaggaat gcccgcctga atcagtaagc aaagcgtacc
    21721 aatgccctgc tggcgggcaa aaataaagta accaagatcg cactgacgaa gtagcgccag
    21781 ataggcgtca aattccagtt tttcgctcaa aatttgcaga ttttcttcgc tgaataactc
    21841 cagccccgcc tgacgaactt cctcaatgta cgcttcgtta ttaggcggat atcccatcgg
    21901 caccaccact tttaccgtat cgccaaattg ctgatgaacg gcgcgcaagg cagcaatatg
    21961 gtcattgctg cggtcaccgg agttccccac cagaatggtc attttccctt cacgttgccg
    22021 atcgttcgcc atcgtattga gagaagggtc catccgcgtc gggaagtaca gcagttcgcc
    22081 ccgcaccttt ggatgcgttt tggcaaaaaa gctcaaatca ccgcgggtgg caaatacaca
    22141 gccgactcgc ttttgcgcca ggcgacgtag cgggtaaaaa agcttatatc tcaagccact
    22201 ggaaagctca tacaggtctg ccccccagat atgccagaaa aactggctgg gcttaatgcc
    22261 accactcagc agagccagcc acagtgtggg attgaactga ccgtggaaaa aaaatcgctg
    22321 ctgacggtta gcttttgctt tcgcgatgac cgcttccgcc agcgattttt tcccagggaa
    22381 aaattgcaca gaaagcgccg gacagctatc acttaagccg tcgtccttgc caacaaccat
    22441 aaactcgcgc gcgtgctcgc tcgtcgcggc cagcgcgtca ttgaaaaacc gcaaaacggt
    22501 tcggttatgg tgagggatat ccgatcccag tacgtgaatc agtacagtca tgcccgccta
    22561 cgccagagta aaaacacgcc acaacaaaga gaaaaataga cgatataagt tgccatatat
    22621 gcctgcgccg cgcccagtgc accatgcgca gggattagcc agtgggcaaa taccatcaat
    22681 aaagtgaact ggctgacttc cgccagaata taaaaccgca gtgacgcttt ggcgatcacc
    22741 agataaccaa agacataagc gcccactttt agcacatcac ccactaactg ccaggcaaag
    22801 agatcgcgca tagcggtaaa tttattcgac aacagcaacc agatagcaaa atcacgcagc
    22861 aaccagacgg taaaactcgc cgccgccact gccggtaaga cgaattttag cgatttaacc
    22921 acttcccggg tgatatcgcg cttttccgtt agccgcgaca acgtgggcag caaatatacg
    22981 ctgaacgatg ccgtaatgaa ttgcaggtag gcatcggaaa tactgctcac cccttgccag
    23041 atccccactt catcccagct atactgcgcc gccagcagtt tacgcatcat gatgtaagca
    23101 acaggcaagg tcaccgacgt aatcaacgcc atgagcgtaa atttgctcaa ctgccctgcc
    23161 agaccgttat cccagctggg tttcagatag cttaacggga tgacaccacg tttgatcaac
    23221 atgatggcgg caggaattac caccagcgcg ggaatcagcg ccagacccag caacgcccct
    23281 tcataaccgc ccaaacggta actgacgtag tacgcgagaa cgccaatcaa gctgccgaca
    23341 atcagcgata acgcattacc tgcggcatcg cgaaagcctt tcatcagcgc cagtaacagg
    23401 ttgccccagg cgatccccat ttgcaccagc gccactaaac gcaccagccc ctgatagtcg
    23461 gtattaccaa acaatccctg gctgattggc gcagctgcca gcacaaaaac cagcgccatc
    23521 agcgtagaga aaccaagtac catcgctgat gaagtgccga ccacgcggcg cagctgttgc
    23581 ggattatcat ggtactgggc aacgtatttg gttacaccgt taaagatgcc agccccggca
    23641 agcacgccga gcacggtaat caactggcgg aaatttgccg ccagcccaag ccccgccgga
    23701 ccaaatgaca ccgccagcaa cttaccgacc agtaacccgg caccaatctt gacctgtgta
    23761 ctggccgccg tccacaagga cgcttttgcc aacgacatat caggagaagt agttcaacaa
    23821 agtcgcaatt accgtacgct gattgacggg cgacaggttg tagaacagcg gcaggcgcag
    23881 caggcgctcg ctctctttgg tggtgtagcg atcttcaccg tggaactcac caaagtgttc
    23941 ccccgcaggg caaccgtgca gcggaatgta atgaaacacc gccatgattt ccgcttcttt
    24001 cagaaagtta atcaacgcgc tccggtcatc aatatcccgc agtttaatgt agaacatatg
    24061 cgcgttctgc acgcagccat cgggaatcga cggcagctcg atacgcccgg ctttcgccag
    24121 aggcgctaac gcatcgtagt aattttgcca cagcgccaga cgttgctggt tgatacgatc
    24181 cgctgcttcc agttgcgccc acaggtatgc agcttgcaga tcggacatca aatagctgga
    24241 gccaatatcg cgccaggtat atttatcgac ctgaccacgg aagaactggc tgcggttagt
    24301 gcccttttca cggatgatct cggctcgttc gattaacgct ttatcgttaa tcagcgtcgc
    24361 gccgccttca ccacccgccg tgtagttttt ggtttcatgg aagctaaagc agccaatatg
    24421 accaatggtt cccagtgcac gccctttgta agtggacatc acgccctgag cggcatcttc
    24481 caccacaaac aaattatgct ttttcgccaa cgccataatg gtgtccattt cgcaggccac
    24541 acccgcgtaa tggaccggca cgataacgcg cgttttgtcg gtgatcgctg cttcaatcaa
    24601 cgtttcgtcg atgttcatgg tgtccgggcg aacatccaca aagacgattt tcgcgccacg
    24661 cagcacaaag gcattggcgg tggagacaaa ggtgtagctc ggcatgatca cttcatcgcc
    24721 aggctggata tcgagcagca gcgccgccat ctccagcgaa gcggtgcagg acggcgtcag
    24781 taacactttg gcgctgccaa aacgttgctc cagccactgc tggcagcgac gggtaaaacc
    24841 gccatcgcca cacagtttgc cgctacccat tgccgactgc atatagtcga gttcggttcc
    24901 caccaccggc ggtgcgttaa atggaatcat gtgatcacct gtataaccag tacgcggtgc
    24961 tttctacatt cgcaccactt tgtatgtatc gtttaagcgc ggcggtgttg cccatttggg
    25021 tcgccacccg caaagttgtt ttaccgcgag catacgccca gtttagcgcc gtttgcatca
    25081 gctcagcacc tgcaccgcgt ccagccagca ggccaattcg cgcatctgtc gcattgagtt
    25141 cccgtaaaga gacatagccg cgaatatcgc cggacgccgc acgtagaatc agacattgat
    25201 gatcaaaggt gccgcgcacg gcattttcaa tccactgtgc ataaaagcga ccgctggcgt
    25261 caggcgcata ccacggcgca cgaaaacggc tttgcgcaaa tgcggcgcta gctaactgac
    25321 gtaatgcggg aatatcggtc tcttgtgcca cgacagcacc gctatcactg acattgttca
    25381 cgggtagcgc caaatcaact tcaccttcta ccagggagaa tcccagctgt tgcagggcat
    25441 ccagttcacc cgtatttgat gccgcaattt tggcctgcac ccgtgaccac ggcgctaacg
    25501 cgtctggcgt caggagcggt gcttcagacg taatgcgcac gatggcgctg ttaacaccaa
    25561 agaaggcgtt ttcccaggtt agtggctcaa tactggcgcg gacgggcacg aagtaactcc
    25621 agcagatatt ggccgtagcc agttttcgct aatgaactgg cagcacgctt cacaccctcg
    25681 tcatcgagcc agccgttacg ccaggcgatc tcttccaggc aggcaatctt aaagccctgg
    25741 cgtttttcta ctgtctgcac aaaggtactg gcttcaatca ggctgtcgtg agtgccagtg
    25801 tccagccagg caaatccgcg cccgagcagt tcaacggtca ggttgcccgc ctcgaggtac
    25861 atctggttga tggaggtaat ctccagttca ccacgctccg acggcttcac ctgctttgcg
    25921 tactccacga ctttactgtc gtagaaataa agcccggtca ccgcccagtt tgacttcggc
    25981 tgttttggct tttcttccag cgagatagcg cggaaattgt cgtcaaactc caccacgcca
    26041 aagcgttcct ggtccatcac ctgatagcca aataccgtcg ccccttccgt gcgcgccgca
    26101 acatgacgca gcttcggact gaagccctga ccgaagaaga tgttatcgcc cagcaccaga
    26161 caagaaggtt caccgttgag gaaggtttca ccgatgataa aggcctgcac cagaccgtcc
    26221 gggctgggct gttcggcgta ttccagctgg ataccgaact cactaccatc gcccagcagg
    26281 cgctggaaat aacctttatc ttccggcgta gtgatgatga gaatttcgcg gataccggcc
    26341 aacatcagca ccgacagcgg atagtaaatc attggcttat cgtaaatcgg caacagttgc
    26401 ttcgatacgc cgcgcgtaat cggatgcaat cgggtgccgg aaccgcccgc caggataata
    26461 cctttcatgt ttgcctccgc tgaaaattag cctttcagac ctaaacgctc gccctgatag
    26521 ctgccgtcct gcacctgctt ccaccagctt tcattagcca gataccactg caccgtttta
    26581 cgcattccac tttcaaaggt ttcctgtggc agccagccaa gttcacgggc aattttcgaa
    26641 gcatcaatgg cataacgcag atcatgcccc ggacggtcag cgacaaaggt gatcaagtca
    26701 cgatagtgca ccacgccgtg cggcttgttc ggagccagtt cttccagcag ctcgcaaatg
    26761 gtttccacaa catcgagatt cttacgctcg ttgtgaccac caatattata ggtttcaccg
    26821 actttcccgg tggtcgccac gcaatacagc gcgcgggcgt gatcttccac atacagccag
    26881 tcacggattt gctgcccgtt gccatatacc ggcagcggtt tacccgccag cgcgttgagg
    26941 atcatcagcg ggatcagttt ttccggaaag tggtaaggac cgtagttatt cgagcagttg
    27001 gtgataagcg tcggcagacc gtaggtccgc agccaggcgc gcaccagatg gtcgctgctg
    27061 gcttttgacg cggaataagg gctgctcggc gcatacggcg tggtttcggt gaagaaatca
    27121 tccgtcgagt gcaggtcacc atatacttcg tcggtggaga tatgatgaaa acggaacgct
    27181 gattttttat cttccgtcag cgtattccag taagcccgcg ccgcttcaag caatgtataa
    27241 gtcccgacaa tgttggtttc aataaacgct gccgggccgt caatagaacg gtcaacatgg
    27301 ctttcggctg ccagatgcat gacacagtct ggctgatgct cagtgaatac gcgtgccagt
    27361 tctgcccgat cgcagatatc aactttctca aaggcaaagc gatcgctttg cgcgaccggt
    27421 gccagcgaca tcaggtttcc ggcgtaggtc agcttatcga ccactaccac cgcgtcgctc
    27481 gtttcgttga tgatataacg caccagcgcc gagccaataa acccggcacc acctgttatc
    27541 agaatttttc tcatcagcgc cagactcctt tggcatcgac gacatactgc tgatggacat
    27601 tgtcgccatt gataacttta aactgactat gatcgaccag catcaccagc acgtctgccg
    27661 ttgccagcgc ctcgtcaagc tgcgccagag tacaaagccc ggtcagtttt ttcggcaact
    27721 ggtggatgtt aggctcaaca accagagttt cgccgctatg ccactgggcg atcagttcag
    27781 cgatttccat cgccgggctt tcgcgcaggt catcaatatt cggtttaaac gccagaccaa
    27841 agcaggcgat tttcagttca ctggcgcgtt tatcggtagc cgccaggcaa tcagccaccg
    27901 ccgctttcac ctgatcgata acccagaacg gtttgtgatc gttcacttcg cgcgcggtac
    27961 ggataagccg cgcctgctgg gggttctgtg ccacgataaa ccacggatca acagcaatgc
    28021 agtgaccgcc cacgccaggg ccaggctgaa gaatattaac gcgagggtga cgattcgcca
    28081 ggcgaatcag ttcccagacg ttaatcccct gatcggcaca aatcagcgac aattcattag
    28141 caaaagcgat attcacatcg cggaagctgt tttcggtgag cttacacatt tccgccgtcc
    28201 gcgagttagt gacgacacac tcaccttcga ggaaaatttt gtacagttcg ctggcgcggg
    28261 tcgaacaaac cggcgtcata ccaccaatca cgcgatcgtt tttaatcagc tcgaccataa
    28321 cctggcccgg taacacgcgt tccgggcagt aagcaatgtt gacgtccgcc tgctcgccca
    28381 cctgctgtgg gaaagtgaga tccggacgca tctctgctaa ccattctgcc atcttctcgg
    28441 ttgaccccac cggcgaggtg gattcaagga tcaccagcgc gccttttttc agcactggcg
    28501 caatggagcg agcagccgat tcaacgtagg tcatatctgg ctcatgatcg cctttaaacg
    28561 gcgtgggtac agcaatcagc caggcatccg cttcaactgg cgtcgtgctc gctcgtaaaa
    28621 aaccgccttc tacggcagtt tttactacgc tcgccaaatc aggctcgacg atatggattt
    28681 cgccacgatt gatggtatca accgcgtgtt ggttgatatc gacaccaatc acctgttttt
    28741 gccgtgaggc aaacgctgct gctgtaggca gcccgatgta acccagtccg ataacagaaa
    28801 tggtcgcaaa actcatagtg atatccgatt attttttaac gcttccagaa tgcgagagca
    28861 tgcctgacca tcaccatacg ggttatgggc gcggctcata gtttgatatt cgttttcgtc
    28921 ttttaaaaga cgcgtcactt cctcgacaat tcgctgctta tccgtgccta ccagacgcac
    28981 cgtacccgcc gtcaccgctt ccggacgctc agtggtatcg cgcatcacca gcaccggttt
    29041 ccccagcgaa ggcgcttctt cctgaatgcc gcctgagtcg gtcaaaatca gccaggcgtg
    29101 gttcatcaac cagacaaacg gtaaatactc ctggggatcg atcagaatga catttttcac
    29161 atgccccaga atgcgattga ccggctctct gacgttcggg ttgagatgca ccggatagac
    29221 aatctggatg tcctggtgcg tggtggcaat gtctgccaac gcgtggcaga tttcttcaaa
    29281 accacgaccg aaactctcac gcctgtgacc ggtcaccaga atcatctttt tattggggtc
    29341 gataaacggg taatttgcca ccagttctga acgcagcgtg tcgctgctca tcacctgatc
    29401 acgcacccat aacagtgcat caatgactgt attaccagta atgaagattc ggctatccgc
    29461 aacgttttca cgcagcaagt tttgccggga agtttcggtt ggagagaagt gatacatcgc
    29521 cagatgcccg gtcaatgtac ggttagcctc ttccggccac ggcgaataga gatcgcccgt
    29581 gcgtaaacca gcctcaacgt gaccaacagg aatacgctga taaaacgccg ccaggctggt
    29641 tgccagcgtc gttgttgtgt cgccgtgaac cagcacgacg tctggtttga actcggcaag
    29701 aataggtttt agcccttcca gaatccgaca ggttatctct gtcaggccct gtcctggctg
    29761 cattatgttg agatcgtagt caggtacaat ggaaaagagt ttcagcacct gatcgagcat
    29821 ctcccgatgc tgcgcagtga cgcaaacttt agcctcaaaa aaaggagctt ttgccaacgc
    29881 atgcaccaac ggagccatct tgatggcttc cgggcgcgta ccaaatacag tcagtacttt
    29941 cacatcgatt ctcttcgaat aagcggcgag cgcctttgcg ctcgccgcag cagtgttgct
    30001 atttcgagca acggcgggtt aatgcgacac cagccccgat cagccccccg acaatgcccc
    30061 acataatcat caggaaggca cgacgtgggc tatcgcgttt taccggttct tccggcgtac
    30121 gcaaatagcg ataggtctga aaacgcggat ccagggttgg accaacattc agggtgttta
    30181 acatggcccg attctgatca tagtcgagat caaaggccgg accgacggcc tgtaaatttt
    30241 ccagtcgagc ctggagcatt ggacgcccaa gcaggaacat ttctgaatca ggtaattcct
    30301 cggcaggcac atctgtcgca ctgcgcgaaa tattatgctg ctcagcaatt ttcagcgcct
    30361 gctcaatgct gttcatccgg cggtcgtaga tggctttcgc cacctcttcc tgacgcttca
    30421 cctgagcttt catctggatg gtgcgtgccg cccatgcgcc tttcagctca tcattcagat
    30481 ggctggctgc acgctggctg gcaaaagcaa catactgacg taacaggtta ttagcgtcag
    30541 gcgcggtttc ggcaataagc ttcacgctgt cattgaccgc gcgggtaaag tctccgggga
    30601 taaactggat gttgttaatc atttcatcca gcaacgccgc atcggctttg ctgttgccca
    30661 ccatccgctg tttgtaatag tcggtttgca gccagaactc tctgcgggta tcccacgagg
    30721 ccagttgcat aacaaactct ttataggctt cgtccatgac cgatggttga tcggcagaag
    30781 ccatgtttga acggacatcc aggttacgca aaaattgctg ctgcgagtaa tatcccccca
    30841 gcatattcac cgttggacga tcggtaatcg ccgtcgagct ccactcctga cgagcaaaaa
    30901 aagtatacgc cagcgcgatt aacgcaaacg ccagccccat gccaataatc catagcttcc
    30961 cagcccacaa ggtacgaaac aacccacgaa tatccagttc attttcagcg tcttcggccg
    31021 gtttcccagg cattggttgt gtcatcacat cctcatttat ttggttaaat tggggctgcc
    31081 accacgattt ctacgcagtc tgcgttttac gcgcttaata aagcgagcaa ctttccaggc
    31141 acgcttaatg caatatccat agaggaagaa tgctagcaaa aagagcacca gcatgaccca
    31201 ctccgggaca aaatgagaat attctgccag cacgccaatg gaagcgagca gtgctgcggc
    31261 aagggtaatc agcacaaacg cctgacggga agtaaacccg gcacgcatga tcaaatggtg
    31321 aatatgctga cggtcaggag agaatgggct catgccttta cgcaggcgac ggtacataat
    31381 cgccaccata tccattagcg gaatggcgat tatccacaaa gcggtaaccg ggctgatggg
    31441 atgggttttg ccctgggtcg tttcgagcag gatccagata acggtaaaac caatcagcgt
    31501 actgcccgca tcacccataa agactttgta gcggcgaccc aggataccaa ggttaagcat
    31561 gatgtatggc aggatggcgg cgatcatcgc aaagcaccag attgcgaggc tggtttgccc
    31621 gtcgaaccac aaaatcatac cgattgctgc aaacgagacg caggacaacc cgcccagcaa
    31681 gccatcaatg ccatcaacca tgttgaacgc attaatggcc gcccagacgg caaatagcgt
    31741 caggaagtaa ccaaacggtc cgagcaccat ctcccaggag ccaaagatat aacccaggct
    31801 actgagataa agtttgccga acaccatcat aacaatgcca acagcggcct gtatggtggc
    31861 acggattttt acgctgatat caaaacggtc atccagcgcg ccaatgaaaa caagcacacc
    31921 ggcacaagcg agatagagag atgcatgcgg aatatagtaa tcgacaattc cgaacgtgaa
    31981 gcaaatccct gcgtaaaccg aaatcccccc aacgagaggt atcaatccct ggtgacgttt
    32041 gcggaagttt ggtttatcca ctaaaccgac tttttttgcc accttacggg caaaaaacag
    32101 aaacagtgtc gtgaataaaa aaatactgat gagatcagta ctcactgtca gtaaattcac
    32161 aatgcatgct ctcagagaaa attattagca gaagtataac cacgaagacc tttattcaga
    32221 agggaaagtc cgttccgaac ccaccagtcc tgtgcaaaaa ttaaccattt gatatgcaaa
    32281 ttgcataaca tgccagcaaa ttccaggaat tttcctaaca tcgccagcgc ggcattaaga
    32341 ttacagatat aaaagcaaaa cgccacgtaa acacgtggcg tttttggcat aagacaaatt
    32401 tatgagcgtt tcatcatttc gaagaaatcg tcattggtct tggtcatggc cagtttatta
    32461 atgaggaatt ccattgcatc gatttcgccc atcgggtgaa tgattttgcg caggatccac
    32521 attttctgca gttcttcctg agtcgtgagc agctcttctt tacgggtacc ggaacggttg
    32581 tagtcgatag ccgggaagac gcgtttttca gcgatcttac gagagaggtg cagttccatg
    32641 ttgcctgtac ctttaaactc ttcgtaaata acttcgtcca ttttagaacc ggtatcgata
    32701 agcgccgtcg cgataatggt caggctgccg ccctcttcca cgttacgcgc cgcaccgaag
    32761 aagcgtttcg gacgatgaag ggcattggcg tccacaccac cggtcaacac tttacctgac
    32821 gccggaacaa cagtgttgta agcgcgcgcc agacgagtga tggagtcgag cagaatgata
    32881 acgtctttct tgtgctcaac caggcgtttg gccttctcga tcaccatttc cgcaacctga
    32941 acgtggcgag atgcgggttc gtcaaaggta gaagcaacaa cttcaccttt taccagacgc
    33001 tgcatctcgg ttacttcttc cggacgttcg tcgatcagca gaaccatcag cacacaatcc
    33061 gggtggttgt aagcaatgct ctgagcaatg ttctgcagca gcatagtttt accggctttc
    33121 ggcggtgcca caatcagacc acgctgacca cgaccgatag gtgatgccag atccagtacg
    33181 cgagcggtta aatcttccgt agaaccgtta ccacgttcca tacgcagacg agagtttgcg
    33241 tgcagcgggg ttaagttctc aaagaggatt ttgttgcggg cgttttcagg tttgtcgaag
    33301 ttaacttcgt taactttcag cagcgcaaaa tagcgttcac cttctttcgg cgggcgaatc
    33361 ttaccagaga tggtatcacc agtgcggagg ttgaaacggc ggatttggct aggggaaacg
    33421 tagatgtcat caggaccggc gaggtaggag ctgtctgcgg aacggaggaa accaaatcca
    33481 tcctgcaata tctccagtac gccatcacca aagatatctt cgccactctt tgcgtgctgc
    33541 ttcaggatgg caaaaataat gtcctgctta cgcatacgag ccaggttttc cagccccata
    33601 ttttcgccga gagtgatcag ctcagaaacc ggcgtattct ttaattcggt aagattcata
    33661 gtggtgtgag ttcttaaact tggggtaaat ctcgaactta atgttgtgaa tggtatggca
    33721 ggatcatcca tgcctgttta cggtcatcga ctcatgtctt ttcgctgcct ggtcacagga
    33781 gagtacgcag aactgaaacg acaagacgga ttgagtgacg agccagaaat ctgttcactt
    33841 cgcatttaag ataaaacggg aagcgttgga aattacaaga ttcaaactta ataaggtatg
    33901 tttaatacga agtcaacact aagttagcat gactcacgcc gggcgtccag tttttagcga
    33961 cggggcaccc gaacatgaaa ttcccttacg ccaggttagc gtcgaggaac tctttcaact
    34021 gacctttaga cagtgcaccc actttggttg ccgccacttc accgtttttg aacagcagca
    34081 gagtcgggat accacggatg ccatatttcg gcgcagtgcc agggttttga tcgatgttca
    34141 gttttgcaac ggtcagtttg ccctgatatt cgtcagcgat ttcatccaga atcggggcga
    34201 tcattttgca cggaccgcac cactctgccc agaaatcgac gaggatcgcc ccgtccgctt
    34261 tgagtacatc cgtgtcaaaa ctgtcgtcag tcaggtgaat aattttatcg ctcatatata
    34321 actccacagg aataagcctg gcgtgttggt ttcgttgttg gtgtaacatt aaccaactaa
    34381 aggttgactt tatttcaccg gatacgcttt cgtaaagcaa tagtaagctg atattctacc
    34441 acactatgag caaaacacat ttaacagaac agaagttttc cgacttcgcc ctgcatccga
    34501 aggttgtaga aatccttgaa aaaaaagggt ttcataactg tacgcccatt caggcactgg
    34561 cccttccgct gacgctggcg ggtcgtgacg tagccgggca ggcgcaaacc ggtaccggga
    34621 aaacgatggc gtttcttacg tcaacgtttc attatcttct ctctcatcct gcgattgccg
    34681 atcgcaaggt gaatcaacca cgtgccttaa ttatggcacc gacgcgtgaa cttgccgtgc
    34741 agatccatgc cgacgcagaa ccgctggcgg aagctaccgg cctgaagctg ggtctggctt
    34801 acggtggtga tggctacgac aaacagctga aagtgctgga aagcggcgtt gacattctga
    34861 ttggcactac ggggcgttta attgactacg ccaagcagaa ccacattaac ctcggtgcca
    34921 ttcaggtggt ggtactggac gaagccgatc gcatgtacga tctgggcttt attaaagata
    34981 tccgctggct gttccgccgt atgccgcctg caaaccagcg cctcaacatg ctgttctccg
    35041 ccacgctttc gtaccgggta cgtgaactgg cgttcgagca gatgaacaat gccgaatata
    35101 ttgaagtgga accggaacag aaaacgggcc accgtattaa agaagagctt ttctaccctt
    35161 ctaacgaaga aaaaatgcgt ttgctgcaaa cgctgatcga agaagagtgg ccagacagag
    35221 cgattatttt cgccaatacc aaacaccgtt gtgaagagat ctggggccac ctggcggcag
    35281 atggtcatcg tgtcggtttg ttgacaggcg atgtcgcgca gaaaaaacgt ctgcgtattc
    35341 ttgatgaatt tacccgtggc gatctggata ttctggttgc caccgacgtt gccgcgcgtg
    35401 gtttgcatat tccggcagtg acgcacgtct ttaactacga tttacccgat gactgtgaag
    35461 attacgttca ccgtattggt cgtacaggtc gcgcaggcgc aagcggtcac tctatcagcc
    35521 tggcgtgtga agagtatgca ttgaatttgc ctgctattga gacctatatt ggtcactcaa
    35581 ttccggtaag caaatacaat ccggacgcat tgatgaccga tctgccaaaa ccgctgcgcc
    35641 tcacgcgccc acgtacaggc aatggtccgc gtcgtactgg cgctccgcgt aatcgtcgtc
    35701 gttcaggtta aataaaatgt catactcaaa atcattcaaa ctgtatcaag atggcaagag
    35761 aatgaatccc gatgagcata ctgtagtaag tgattcaggt gaaagaatgc agccaacaca
    35821 gagacagatt gaaggatgaa gagtatgggt tccacctcgt cgctgtatgc agccattgat
    35881 ctcggttcga atagttttca tatgctggtt gtgcgcgagg tggctggaag catccagacg
    35941 ctgacgcgaa taaaacgcaa agtgcgtctg gctgctggcc tgaatagcga aaatgccctg
    36001 tctaatgaag caatggagcg cggttggcaa tgtctgcgcc tgtttgctga acgtctgcaa
    36061 gatatccctc cctcgcaaat tcgcgttgtc gctacggcga cattacgtct tgccgtcaat
    36121 gcgggtgatt ttattgccaa agcgcaggaa atcctcggtt gtccggtaca ggtgatcagc
    36181 ggtgaagagg aagcacgtct gatttatcag ggcgttgctc acacaactgg cggtgccgat
    36241 cagcgcctgg tggtggatat aggcggtgcc agtactgaac tggtaaccgg cacgggtgca
    36301 caaaccacct cgttgttcag cctgtcgatg ggctgcgtca cctggctgga acgctatttt
    36361 gccgatcgta atctggggca ggaaaatttt gatgctgcag aaaaagcggc acgcgaagtg
    36421 ttacgtccgg ttgccgatga attacggtat cacggctgga aagtgtgcgt tggtgcttcc
    36481 ggcaccgtgc aggcgttaca ggaaatcatg atggcacagg ggatggatga acgcattacc
    36541 ctggaaaagt tgcagcaatt gaaacagcga gccattcatt gcggtcggct ggaggaactg
    36601 gagattgacg ggctgacgct ggaacgtgcg ttagtgttcc cgagtggtct ggcgatcctg
    36661 atcgccattt ttaccgaact gaatattcag tgtatgaccc tggcgggcgg tgcgctgcgt
    36721 gaaggcctgg tctacggcat gttgcatctg gccgtcgagc aggatattcg cagccgtacg
    36781 ctgcgtaata ttcagcgccg ctttatgatc gatattgatc aggcgcagcg cgtagccaaa
    36841 gttgcggcta acttcttcga tcaggtggaa aaggaatggc atcttgaagc aataagccgc
    36901 gatttgctca tcagcgcctg tcagcttcat gaaatcggcc tgagcgttga ctttaaacaa
    36961 gcgccgcaac acgctgctta tctggtgcgt aatctggatc ttcccggttt tacccccgca
    37021 cagaaaaaat tgctggcgac gctactgctc aaccagacta atccggtcga tctctcatcg
    37081 ctgcatcagc aaaatgccgt accgccgcgc gtcgcagaac aactctgccg tttactccgc
    37141 ctggccatca tttttgccag ccgtcgccgt gacgatctcg tgccagagat gacattacag
    37201 gctaaccatg aactgttgac cttgacgctt ccgcaaggtt ggctaaccca acatccgctg
    37261 ggtaaagaga ttattgctca ggaaagccag tggcagagct atgtccactg gccgctggaa
    37321 gtgcattaat gagtaagtgc cggatgcgat gctgacgcat cttttccggc cttgattatt
    37381 tccctcgttt tgccgccatc atcgctttca gattcgccag atggctttgc cctttctgca
    37441 tccgttcttc ggcgctgacc actttgcgct cctgttccca aatcagatca tcctgcggca
    37501 gctccagcaa aaagcggctc ggctccgggc gcaccagctc gccgtactga cggcgttctt
    37561 tacacagcgt aaaggtcaat tccttctggg cgcgggtaat gccgacatag gccagccgcc
    37621 gctcctcatc gatattatct tcatcgatgc tgctctggtg cggcaaaaac ccttcttcca
    37681 taccgaccat gtagacataa ggaaactcca gcccttttga cgcgtgcaga gtcatcagtt
    37741 gcacctgatc cagctcttct tcactctcac cacgctccat catgtcgcgc aaagtaaagc
    37801 gcgtcaccac ctgggtgagc gtcatcggct catccagttc gctgccttcc agcatctccg
    37861 tcatccagct aaacagttgg ttgacgttct tcatgcgcat ttcggcggct ttcgggctgg
    37921 gcgatgtttc gtacagccag gattcataat ccatgccgtg gatcagatcg cgcaccgcgg
    37981 caatcggctc ccgctccgcc agacgctgga tttctgccaa ccagtgagta aagcgggtca
    38041 atgcttcata accacgtccg ctaagcgtct gactcaggcc catatcaaag ctggcggtaa
    38101 acatgctttt attgcgcgtc atcgcccact cacccagctt tttcagcgtc gccgggccaa
    38161 tctctctctt cggcgtgtta acgatacgca gaaatgcgct gtcatcgtcc gggttagtca
    38221 gcacgcgcag ataagccagc aagtccttga tttcaggacg agagaaaaac gacgtaccac
    38281 cagaaatttt gtacgggatg cggttttgca tcaggaattt ttcaaacact cgcgactgat
    38341 ggttaccgcg ataaagaata gcgtaatatt tgtactgcgt tttattgacg aagtgatgcg
    38401 caatcagttc accagtgacg cgctcggctt catgttcttc gttattcgcg cttaaaactt
    38461 taagctctgt gccgtaaccc agttcggaga acagacgctt ctcaaagacg tgcgggttat
    38521 tggcgatcag gatgttcgcc gctttcaaaa tacgtccgga agagcggtag ttctgctcaa
    38581 gcttaatcac cttcagcgcc ggaaaatcct gactcagcag caccaggttt tgtggacgtg
    38641 cgccgcgcca ggagtagatc gactggtcgt catcgcccac cacggtaaag cgcgcgcggc
    38701 tgcccaccag cagtttcacc agttcatact ggctggtgtt ggtgtcctga tactcatcca
    38761 ccagcagata gcgaattttg ttctgccagc gctcacggac ttcttcattg cgttgcagca
    38821 gcaacgtcgg caataaaatc agatcatcga agtcgagaac attgcacgct ttcaggtgtg
    38881 catcatacag cccataacaa tgggcaaaaa tacggtcccg ctcgccaatc gcactggctg
    38941 ccgcctggga cggtgttttg agatcgttct tccagttaga gatggtcgaa atcaactgtt
    39001 gcaggagaac tttgtcatct tcaatcagcc cttcggtcaa ctctttgagc aacgccagct
    39061 gatcggtatc gtcaaacagc gaaaagttcg ccttcatccc cagcgccgca tactcacgct
    39121 tgatgatatc cagccccagc gtgtggaagg ttgagatcat cagcccacgc gcctctttgc
    39181 gacccagcgt ctgtccgaca cgctctttca tctcgcgcgc cgccttatta gtaaaggtca
    39241 ccgccgcgat atgtcgcgcc tgataaccgc aaccgcggat cagatgggcg attttattgg
    39301 tgatcacacg agttttaccg gaacccgcgc ccgccagcac caggcagggg ccggtaacga
    39361 attcgacagc ttgttgttgg ccggggttta gacgcatagg tgtattgctc aatcttcgaa
    39421 cggggggagg attgtagcag aatcgcattt gaatgtgacc gcgtgtaagc cgctaaccgg
    39481 ttggctgcgg atgtttaaat cagagaggga agaccgaaac cggagttcat cgtgaatcca
    39541 ttagacttag aaaatatcgg gtcgtaacgt tgtgttatta ctttttcaga gtttactaag
    39601 gagaggtgta ctcgtggcga ttaatttcag tcctaaggtt ggtgaaatac tggaatgcaa
    39661 ctttggtaac tatcctgtta gccagaatgg acagttcagc accacgtatt acgatggccg
    39721 cattcctcct gaaatgataa aaaacaggtt agt
//
LOCUS       DAJTMC010000016        37823 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.016, whole genome shotgun sequence.
ACCESSION   DAJTMC010000016 DAJTMC010000000
VERSION     DAJTMC010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37823
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.016"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            <1..392
                     /locus_tag="OO130_000751"
     CDS             <1..392
                     /locus_tag="OO130_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5364264.1"
                     /translation="HYPLICSNKHPRITDNSTYEQIMAEEFTQLISKSAGVDDIQMEI
                     DEKFMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNSHRDFLNLKEIKPEHPLPSI
                     KLYISYNKSSLNNLVFSRFIDRLNESF"
     gene            539..2056
                     /locus_tag="OO130_000752"
     CDS             539..2056
                     /locus_tag="OO130_000752"
                     /inference="COORDINATES: protein motif:HMM:NF014273.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HCR5364265.1"
                     /translation="MSIYKIPLPLNILEAARERITWTLNTLPRVCVSFSGGKDSGLML
                     HLTAELARQMGKKICVLFIDWEAQFSCTINYVQSLRELYTDVIEEFYWVALPLTTQNS
                     LSQYQPEWQCWEPDVEWVRQPPQDAITDPDFFCFYQPGMTFEQFVREFAEWFSQKRPA
                     AMMIGIRADESYNRFVAIASLNKQRFADDKPWTTAAPGGHSWYIYPIYDWKVADIWTW
                     YANHQSLCNPLYNLMYQAGVPLRHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVS
                     GVKSGGIYAGHDNHFYGHRKILKPEHLDWQEYALLLLNSMPEDDRIKAINEIRMAIHQ
                     VSPFREEPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLQKSIEIDGFTQPIVVTHTDK
                     NAMEIVDGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNRARGRHQIT
                     AMSEIVRELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(2057..3206)
                     /gene="ybdL"
                     /locus_tag="OO130_000753"
                     /pseudo
     CDS             complement(2057..3206)
                     /gene="ybdL"
                     /locus_tag="OO130_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308666.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
     gene            3315..4403
                     /gene="hcxA"
                     /locus_tag="OO130_000754"
     CDS             3315..4403
                     /gene="hcxA"
                     /locus_tag="OO130_000754"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HCR5364266.1"
                     /translation="MPHNPIRVVVGPANYFSHPRSFNHLNDFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAVPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALA
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNQAEIDKVIAHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(4413..4610)
                     /locus_tag="OO130_000755"
     CDS             complement(4413..4610)
                     /locus_tag="OO130_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HCR5364267.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(4792..6897)
                     /gene="cstA"
                     /locus_tag="OO130_000756"
     CDS             complement(4792..6897)
                     /gene="cstA"
                     /locus_tag="OO130_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein CstA"
                     /protein_id="HCR5364268.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPKRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLALFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFSITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKDPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(7078..7491)
                     /gene="entH"
                     /locus_tag="OO130_000757"
     CDS             complement(7078..7491)
                     /gene="entH"
                     /locus_tag="OO130_000757"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HCR5364269.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(7494..8240)
                     /gene="entA"
                     /locus_tag="OO130_000758"
     CDS             complement(7494..8240)
                     /gene="entA"
                     /locus_tag="OO130_000758"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HCR5364270.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFTQEQY
                     PFATEVMDVADAGQVAQVCQRLLAETERLDVLINAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(8240..9097)
                     /gene="entB"
                     /locus_tag="OO130_000759"
     CDS             complement(8240..9097)
                     /gene="entB"
                     /locus_tag="OO130_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HCR5364271.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFELQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPVPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(9111..10721)
                     /gene="entE"
                     /locus_tag="OO130_000760"
     CDS             complement(9111..10721)
                     /gene="entE"
                     /locus_tag="OO130_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HCR5364272.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GELQLSYRELNQAADNLACTLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVTEHSSIRVVQLHNDSGE
                     HNLQDAINHPADGFTATASPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIEKHQVNVT
                     ALVPPAVSLWLQALAEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLSQWLASRASA"
     gene            complement(10731..11906)
                     /gene="entC"
                     /locus_tag="OO130_000761"
     CDS             complement(10731..11906)
                     /gene="entC"
                     /locus_tag="OO130_000761"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HCR5364273.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKVQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QASARRFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVLASSPLGEWRETGVKLSTMLNVFGLH"
     gene            12281..13237
                     /gene="fepB"
                     /locus_tag="OO130_000762"
     CDS             12281..13237
                     /gene="fepB"
                     /locus_tag="OO130_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HCR5364274.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAAVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPTGLNASQSQGKRHDIIQLSGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLERLKALF"
     gene            complement(13241..14491)
                     /gene="entS"
                     /locus_tag="OO130_000763"
     CDS             complement(13241..14491)
                     /gene="entS"
                     /locus_tag="OO130_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HCR5364275.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWLVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAEIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDS"
     gene            14602..15606
                     /gene="fepD"
                     /locus_tag="OO130_000764"
     CDS             14602..15606
                     /gene="fepD"
                     /locus_tag="OO130_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HCR5364276.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            15603..16595
                     /gene="fepG"
                     /locus_tag="OO130_000765"
     CDS             15603..16595
                     /gene="fepG"
                     /locus_tag="OO130_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HCR5364277.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLETSQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIVTSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            16592..17407
                     /gene="fepC"
                     /locus_tag="OO130_000766"
     CDS             16592..17407
                     /gene="fepC"
                     /locus_tag="OO130_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HCR5364278.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIKHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTHWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLERTAPSTANS"
     gene            complement(17404..18537)
                     /gene="fepE"
                     /locus_tag="OO130_000767"
     CDS             complement(17404..18537)
                     /gene="fepE"
                     /locus_tag="OO130_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HCR5364279.1"
                     /translation="MSSLNIKQGSDAHFSEYPLASPSSNEIDLLNLIEVLWRAKKTVM
                     AVVFAFACAGLLISFILPQKWTSSAVITPAEAIQWQDLEKTFTKLRVLDLDVNIDRGG
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYTSWTLSFTAPTSKEAQTVLSGYIDYISALVVKESIENVRNKLEIKTQFE
                     KEKLAQDRIKTKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKANVNDVNFTPFKYQLSPSLPVKKD
                     GPGKAIIVILSALIGGMVACGSVLLRYAMASRKQDAMMADHLV"
     gene            complement(18752..22633)
                     /gene="entF"
                     /locus_tag="OO130_000768"
     CDS             complement(18752..22633)
                     /gene="entF"
                     /locus_tag="OO130_000768"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HCR5364280.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGEVDAPLL
                     ARAVVDGLAQADTLRMRFTEDNGEVWQWVDDALTFELPEIIDLRTNIDPHGTAQALMQ
                     ADLQQDLRVDSGKPLVFHQLIQVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCTW
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLPGR
                     SASADILRLKMEFTDGEFRQLATQLSGVQRTDLALALTALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGDEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPDAPALADARYQFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     ADVPDLTNLCYNAPLKPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYLLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQSCATLKQVFCSGEALPADLCREWQQLTSV
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFVPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPLLPLSANGKLDRKALPLPELKAQAPGRAP
                     KAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQVARQVTPGQVM
                     VASTVAKLATIIDAEEDSTRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYI
                     DPQWSIIGIQSPRPNGPMHTAANLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAEL
                     DIYRQDCAHVDIISPGAFVKIGPIIRATLNR"
     gene            complement(22630..22848)
                     /gene="ybdZ"
                     /locus_tag="OO130_000769"
     CDS             complement(22630..22848)
                     /gene="ybdZ"
                     /locus_tag="OO130_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HCR5364281.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQESCQQWLEAHWRTLTPANFTQLQEAQ"
     gene            complement(22851..24053)
                     /gene="fes"
                     /locus_tag="OO130_000770"
     CDS             complement(22851..24053)
                     /gene="fes"
                     /locus_tag="OO130_000770"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HCR5364282.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWREPQGSEEYS
                     PIKRVWVYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQA
                     PEIPAKEIIWKSERLKNSRRVWIFTTGDATAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKAIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            24296..26536
                     /gene="fepA"
                     /locus_tag="OO130_000771"
     CDS             24296..26536
                     /gene="fepA"
                     /locus_tag="OO130_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HCR5364283.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYSLTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGANTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SVNTHF"
     gene            26711..27331
                     /gene="entD"
                     /locus_tag="OO130_000772"
     CDS             26711..27331
                     /gene="entD"
                     /locus_tag="OO130_000772"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HCR5364284.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKAEHLAGRIAAIYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGATALAVVSRQ
                     PIGVDIEEIFSAQTATELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(27453..27605)
                     /gene="hokE"
                     /locus_tag="OO130_000773"
     CDS             complement(27453..27605)
                     /gene="hokE"
                     /locus_tag="OO130_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HCR5364285.1"
                     /translation="MLTKYALAAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            28059..29177
                     /locus_tag="OO130_000774"
     CDS             28059..29177
                     /locus_tag="OO130_000774"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HCR5364286.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSILIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQVTVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            29243..29491
                     /locus_tag="OO130_000775"
     CDS             29243..29491
                     /locus_tag="OO130_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HCR5364287.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPTPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            29556..29924
                     /locus_tag="OO130_000776"
     CDS             29556..29924
                     /locus_tag="OO130_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCR5364288.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            30018..30671
                     /gene="nfsB"
                     /locus_tag="OO130_000777"
     CDS             30018..30671
                     /gene="nfsB"
                     /locus_tag="OO130_000777"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HCR5364289.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNERKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            30779..32026
                     /gene="ybdG"
                     /locus_tag="OO130_000778"
     CDS             30779..32026
                     /gene="ybdG"
                     /locus_tag="OO130_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HCR5364290.1"
                     /translation="MQDLISQVGDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESILNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPAGNDIRSLAGAFKQ"
     gene            complement(32094..33470)
                     /gene="pheP"
                     /locus_tag="OO130_000779"
     CDS             complement(32094..33470)
                     /gene="pheP"
                     /locus_tag="OO130_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HCR5364291.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYICSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNEVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSHRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(33572..36699)
                     /gene="cusA"
                     /locus_tag="OO130_000780"
                     /pseudo
     CDS             complement(33572..36699)
                     /gene="cusA"
                     /locus_tag="OO130_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
     gene            complement(36711..>37823)
                     /gene="cusB"
                     /locus_tag="OO130_000781"
     CDS             complement(36711..>37823)
                     /gene="cusB"
                     /locus_tag="OO130_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HCR5364292.1"
                     /translation="AERKILFWYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASG
                     VRIDPTQTQNLGVKTATVTRGPLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLT
                     VGDKVQKGTPLLDLTIPDWVEAQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRR
                     LIATQKIQTRFTLKAPIDGVITAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIA
                     WLVKDASQFTLTVPARPDKTLTIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNA
                     WLQLNTASEPMLLIPSQGLIDTGSEQRVITIDADGRFVPKRVAVFQASQGVTALRSGL
                     AEGEKVVSSGLFLIDSEANISGALERMRSESATHAH"
BASE COUNT         9074 a        10552 c         9629 g         8568 t
ORIGIN      
        1 gtcattaccc tctaatttgt agtaataagc atccgcgaat tactgacaat agcacttatg
       61 aacaaattat ggcagaagag ttcacccaat taatatccaa atcagcagga gttgatgata
      121 ttcaaatgga aatagacgag aagtttatga atcgcaaaat ttcatttcgt ggttcctctt
      181 tgctgacaat aattaatagc attgcagtta cagatttact gggaatagtc ccctatgaac
      241 tttataactc ccaccgtgac tttcttaatt taaaagagat aaaaccagaa catccgttac
      301 cttcaataaa gctttacatt tcctataata aatcttcttt aaataatttg gtgttctccc
      361 gatttattga ccgtctaaat gagagttttt gatatataac tacagacagc tactataact
      421 ttcatctatt tattcacagc gcgaatgaaa aataaaacac tctgctttac agtgtttact
      481 ttataattaa cagtcttatc tcactgttca ccctgtaacg ttcagagagc gtcttcagat
      541 gtctatttat aaaattcctc ttccgctcaa tattctggaa gctgcacgag agcgtattac
      601 ctggacactc aacaccctac cccgcgtttg tgtctcattt tctggcggca aagactctgg
      661 tttaatgctg catctgacag ccgaacttgc ccgacaaatg ggcaaaaaaa tctgcgtttt
      721 gtttatcgac tgggaggcgc aattctcttg cactattaac tatgttcagt ccctgcgcga
      781 gttgtacacc gatgtcatcg aagagtttta ctgggttgcg ctcccgctta cgacgcaaaa
      841 ttccctttca caataccaac ccgaatggca gtgctgggaa cctgatgtcg aatgggtgcg
      901 tcagcccccg caagatgcga taaccgatcc tgactttttc tgcttttacc agccaggcat
      961 gaccttcgaa caatttgtac gtgagtttgc cgaatggttt tcacaaaaac gtccggcggc
     1021 gatgatgatc ggcatccgtg cggatgagtc ctacaaccgt tttgtcgcca tcgccagttt
     1081 aaataaacaa cgttttgccg acgataaacc atggactacg gccgcaccag gcggtcatag
     1141 ctggtacatt taccccattt acgactggaa agtggctgat atctggacct ggtatgcaaa
     1201 tcatcaaagt ctctgcaacc cactgtataa cctaatgtat caggcaggcg ttcctctgcg
     1261 tcatatgcga atttgcgaac cttttggccc ggagcaacga caaggattat ggctctatca
     1321 cgttatcgaa ccggatcgct gggctgctat gtgcgcacga gtcagcgggg taaaaagtgg
     1381 cggcatttac gccggacatg acaatcattt ctatgggcat cggaaaatcc tcaagccaga
     1441 acatttagac tggcaagaat atgcattatt gctgctcaat agcatgccgg aagatgatcg
     1501 catcaaagcg attaatgaaa tccgcatggc gatccatcag gttagcccct ttcgtgaaga
     1561 gccagtggat tgcgttctgt gggttaaaaa cagccaactt atgcctaatg attacaaccc
     1621 aaacaacgtg gcaccacccg aaaaaaaact gctgcaaaaa tccattgaaa tagatggttt
     1681 tacccaaccc attgtggtca cacatacgga taaaaatgcc atggaaatcg tggatggttt
     1741 tcaccgccat gaaattggta aaggctcatc atcattaaaa ttacgcttaa aaggttattt
     1801 gccggtgacc tgtctggaag gtacgcgtaa tcaacgcatc gccgcgacta ttcgtcataa
     1861 tcgcgctcgt ggacgccatc aaatcaccgc catgtcggaa atcgtccgtg aacttagcca
     1921 gttgggatgg gacgataata aaattggcaa agagctggga atggacagcg acgaagtgct
     1981 gcgcctgaag caaattaacg gcctgcaaga gttgtttgcc gaccgtcaat attcccgcgc
     2041 ctggacggtt aaatagctaa agctggcgca ggcgttcagc tgctgccagc aacgtcgatt
     2101 ccttcttggc aaaacagaga cgaatcagtt tatgtgggaa gggatcggcg caaaacaccg
     2161 acagcggaat cgccgccacg ccgtgctccc gcgtcagcca ctggcaaaac tcaacatcat
     2221 ccagggtaga aacctcgctg taatccacca gcaaaaagta tgtaccttca cacggtaaaa
     2281 tctccagccg gctttcattt aaagcattca ccagaatatc gcgcttctgg cgataaaagt
     2341 ccggtaacgc aagataatgc tcaggttctg cacgtagcat atcagcaagc gccagctgtg
     2401 ccggggtatt caccgaaaag gtcagatact gatgtacctt gcgaatttcg gcgctgatgg
     2461 gcgctggcgc aacacaataa cccactttcc agccggtcat atgatagatc ttgccaaatg
     2521 aagaaaccgc cactgcccgc tcacgcagct gcggatgcgc cagcacactg gcatggccct
     2581 gttgtgaaaa gttgatgtgc tcgtagactt catcgctaat gacaaaaatc tcgtgcccgg
     2641 cgatcgcctg ccacaaagcg gcgaaatcag cctgctgcca gacagttgca ctggggttat
     2701 gcggagtgtt gaggatcacc agtctggtgc gctcgcttaa taatgcggca aattcctgcc
     2761 agtcaaggtg gttgcagtgc catacgcttc actattcccc cagaaagcgc gatggcgggg
     2821 gcgtaactgt catagctggg atcaaaacaa atcacttcat cgccattgcg caccagtgcg
     2881 gtaatcgccg cgtataacgc ttccgtcgcc cctgccgtta cggtgatatc gctatcggca
     2941 tctggttgat agccatacaa acgttccgtt ttctgagcaa tcgcctcgcg caaggcctgc
     3001 acgccggtca tgggcgcgta ttggtttgcc ccctgggcaa cgtggtacgc cagccgctcc
     3061 tgtaaatagc gcggaccatc aaaatcagga aagccttgcg acaggttaat cgcctggtgt
     3121 tgctgcgcca gcgcgctcat ctgggtgaaa atagtggtgc caagttgtgg aagtttgctt
     3181 tgtggaatca gagggttatt tgtcattttt attgtacctg acgaagttgc gtgttgaagg
     3241 cactataaca cgatgctagt atttggcaat caagacgttt agatgtctaa ataaaacaat
     3301 aaggacaaca caacatgcct cacaatccta tccgcgtggt cgtcggcccg gctaactact
     3361 tttcacatcc aagaagtttc aatcacctga acgatttttt cactgatgaa caactttctc
     3421 gcgcggtgtg gatctacggc aaacgcgcca ttgctgcggc gcaaaccaaa cttccgccag
     3481 cgtttggact tccaggggca aagcatattt tgtttcgtgg tcattgcagc gaaagcgatg
     3541 tacagcaact ggcggctgag tccggtgacg atcgcagcgt ggtgattggc gtcggcggcg
     3601 gcgcactgct cgacaccgcg aaagccctcg cccgccgtct cggtctgcca tttgttgccg
     3661 ttccgacgat cgccgctacc tgcgccgcct ggacaccgct ctccgtctgg tataacgatg
     3721 ccggacaggc gctgcattat gagattttcg acgacgccaa ttttatggtg ctggtggaac
     3781 cggagattat cctcaatgca ccgcaacaat atctgctggc ggggatcggt gacacgctgg
     3841 cgaaatggta tgaagcggtg gtgctggctc cgcaaccaga aacgttgccg ctaaccgtgc
     3901 ggctggggat caataatgcg caggccattc gcgacgtctt gttaaacagt agcgaacagg
     3961 cacttgccga tcagcaaaat cagcagttaa cgcaatcatt ttgcgatgtg gtggatgcta
     4021 ttattgctgg tggtgggatg gtcggtggtc tgggcgatcg ttttacgcgt gtggcggcgg
     4081 ctcatgccgt gcataacggt ctgactgtcc tgccgcaaac cgagaagttt cttcacggca
     4141 ccaaagtcgc ttacggaatt ctggtgcaaa gcgccttgct gggtcaggat gatgtgctgg
     4201 cgcaattaac cggggcgtat cagcgttttc atctgccgac tacgctggcg gagctggaag
     4261 tggatatcaa taatcaggcg gagatcgaca aagtgattgc ccacaccttg cgcccggtgg
     4321 agtccattca ttacctgcca gtcacgctga caccagatac gttgcgtgca gcgttcgaaa
     4381 aagtggaatc gtttaaagcc tgacgtgcaa atttagcagc agcgcgcgcc gccttttcca
     4441 ccgtagcgcg cgtcctgccg ctcacggaaa aactcctcgt aggtcatcgg cgtttgatcg
     4501 ggatggttaa cccgcatatg ttcgacatag ttgtcgtaat caggcatacc aatcatcagc
     4561 ttcgccgcct gacctaaata ttttccggct tttgccagtg aatcaaacat cttggcctcc
     4621 gctaaccatg ttgctgaaag agtgccggat gcggcgtgaa cgctttatcc ggcctacaaa
     4681 tgctatcaac gcactcggtc agtcccctcg ccccatcggg gagagggtta gggtgagggg
     4741 gaaccctcgg cactatgcca acattcgcca acatcccccc tcactctgac tttagtgcgc
     4801 gcctttcgcc tgcgctacga tctcctcgac attttccggc atcggctcat acggcgtttc
     4861 tttcgccgtt ggcttcggat ctttcaatgc cgcaagtgcc gtcttaatcg agaacagtgc
     4921 cagaaccacc acgaccacca taaagaagat ggttaacccg gcatccagac ggttgttgaa
     4981 caccagttgc gccagctgtg actcagtata ctgcgacgga atattgccgc tgtcgatcat
     5041 tgcctggaac ttgttagcaa tggccaggaa gccgactttc gcatccgggc taaacgcttt
     5101 ctgccagcca gcggtcaggg tacaaatcag cagccaggcc gttggtacca gcgccaccca
     5161 ggcgtaacgt tgacgtttca tcttgaacaa caccacggca cagagcatca gcgccatccc
     5221 tgccagcatc tggttggcaa taccaaacag cggccacaga gtgttaatac cgcccagcgg
     5281 atcgaccacg ccctgatgga ggaagtagcc ccacgccagt acgcacagcg ccgttgccag
     5341 caggttagca ggcagtgaat cggtccgctt caggccagga gacaccacgc ccagcagatc
     5401 ctgcaacata aagcgcgcag cacgcgtacc cgcatctacc gccgtcagaa taaacagagc
     5461 ttcaaataga atggcgaagt gataccagaa cgccacatcc atcataccac ccagcgcacc
     5521 gtgcaggatg taagccatcc ccaccgccaa cgtcggcgca ccgcccgcac gggaaatgat
     5581 cgactgttca cccacttcgc tggcaatctg gttgagcgta tctggtgtaa tgctaaagcc
     5641 ccagctactc accacctgcg cggcagaagc gaccacatcc gccgtccctg ctggagccag
     5701 caccgccatt gggctgttca tggcaaagta aacgcccgga tcgatgatac aggcggaaac
     5761 cagcgccata atcgccacga aggattccat taacattcca ccgtagccga taaaacaagc
     5821 ctgcccttcg ttcgccagca tcttcggcgt ggtgccggaa gagatcagcg catggaagcc
     5881 agacaccgcg ccacaggcga tggtgataaa caggaacggg aacaggttac cggtccatac
     5941 cgggccagtg ccatcgacaa atttggtcag cgcaggcatg gtcagcgtcg ggcgcataat
     6001 caaaatgcct accgccagac cgacgatcgt cccgattttc aggaaggtag agaggtagtc
     6061 acgcggggcc agcagcaacc acaccggcag caccgccgcc acaaaaccgt aacccaccag
     6121 catccaggtc agctgcacgc cggtaaagtc aaagtacggt gcccaggtcg gactttctgc
     6181 cacccagccg ccagagataa tggcgaaaat caggaatacc agaccgatga ccgacacttc
     6241 accaatacgc cccggacgca gatagcgcag gtagatcccc ataaacagcg ccagcggaat
     6301 ggtgaacgca acggtatacg ttccccacgg gctatgagtc agggctttca ccacgatcat
     6361 cgccagtact gcaaggataa tgaccatgat cataaagcag gccaccagcg ctatcacccc
     6421 ggcggttggc cccatctctt ctttgaccag ctcacccagc gatcgaccgt cacggcgcgt
     6481 agaaacaaac agcaccatga aatcctgcac cgcaccggcg agaaccaccc cggcgagtag
     6541 ccagatcatc cccggcaggt agcccatttg cgctgccagc accggcccaa ccaacggacc
     6601 tgctccggcg atggccgcaa aatggtgacc gaacagcact ttcttgtccg tcggcacata
     6661 gtccagcccg tcgttatggc gcaccgctgg cgtcatacgc ttcgggtcaa ccgccagcac
     6721 atttttggcg atatacaacc cataaaaacg gtaagcgatc agatagatac agaccgacgc
     6781 caccacaatc cacagcgcgt tgatctgttc cccacgattt aaagcaatgt atcccagagc
     6841 aaatgctccc attacagaga gcactgtcca gacgaggtat ttccctgatt tgttcatagt
     6901 tgttatccgt gtgcgtgtcc atagagatgt tacattttgt ttctataaca tctttgtacg
     6961 atttaacaac acggtaacta tgtaaatcgg agttgaaacc aatatttaac ttaacaatgt
     7021 taactcgatc actccgttaa ccgagttttc ctgcaatctc atttaattct gtcgggctca
     7081 tcccaaaact gccgtaccca gccgacaagt gcagcaacgc cgcccctgtt catcgaaaac
     7141 gacgatttcc cagctctgat tttgccgccc aagatgcagc ggctggcaga cgccgcgtac
     7201 cttgccttca gacaccgggc gatgatgggt ggcgttaagt tccgtgccca ccacacactg
     7261 cccgtcacgg gtcatcataa atccggccat cgatcccagc gtttcagcca gcgccgccga
     7321 cgcgccgcca tgcagcaggc caaacggctg atgagtacgg gtatcaaccg gcatttcggc
     7381 ttccagcaca tcatctccca gacgggtata cacaattccc agatgcgcca ccattgtgtt
     7441 atcgctggtg gcgttcagtt cgtcgagcgt taaatggcgt ttccagatca tgcttatgcc
     7501 cccagcgttg agccgccatc gaccacaata tcctgtaggg tgatatggct ggcgaggtca
     7561 gaggcgagga acaaaatcgt gttggcgatc tcttgtggac gggcgatttt ccccagcgga
     7621 atgccgagtt taaactgctc gccaaagccg cgaatacgct gttcttcggc gtcatcgctc
     7681 acccacagcg tgcgttgcat atcggtgtcg gtggagccag gcgaaaccac attacagcgc
     7741 acgccgctac ccgccagttc cagcccgacg ctcaacgcca ggcttttcag cgccgctttc
     7801 gatgcgccat aagcactcat accaatacgc ggcgtgtgtg cggcgtcgga cgccacagtg
     7861 acaatcgccc cgccccgctg acggcgaaac tggttcatgg tttgctggaa caggttaaac
     7921 gcaccgccga cgttaaccgc aaaagtctgc tgccagtcct ctttactgag ctgatcggtc
     7981 gcgcccatgc gcaaaattcc cgccgcattg atcagcacgt ccagtcgctc cgtttcagct
     8041 aacagtcgct gacatacctg cgccacctgc ccggcgtcgg caacatccat tacttcggtc
     8101 gcaaagggat attgctcctg agtgaacgct tgatcaaaac ctgtaacttt cgctcccgcc
     8161 tcaacaaacg ccagcgccgt ggcgtagccg atacctttac ctgcgccggt tacccagaca
     8221 tttttaccgc tgaaatccat tatttaacct cgcgggagag tagcttccac caggcgtcga
     8281 tggtcgggtt tttcgccagc atgacaaagt cgatatcacc atgcactttg cgccagcgcg
     8341 ccgccagcgc catcatgcgc accgaatcca gaccgtagtc gatcaggttg tcgtcatcga
     8401 acggttcatc ggactcgtcc agcaacggca ggataacctc acgcagcgcc gctttgctgg
     8461 cggggacagg tgctggcagt aattcttcag tcatcaccac ccggccagac cgcccggcca
     8521 catatttcag cgacatcaaa tgctcgtcac ggctgaaatc ggccagcgca tccgccacca
     8581 taaacggttt aatatcgcgc ataaatgcgt cggttgcggt ggtcatacag ccaatgtggg
     8641 catatacccc ggtaataatc agctggttac gtccactctc tttgagcatt tgctccagcg
     8701 gagaacgatg aaacgcgctg tagcgccact tcaccagcac cgtgtcgtcg gcatctggcg
     8761 tcaggcgatc caccaccttt tgctgttccg gcgaacgggt caggcccggc ccccacatat
     8821 cattcaacag cgcccgatct tcatcgctct gctctttcgg ctgggcggtg taataaactg
     8881 ggatattgtg ctgtttgcag tagtcgcgca gcgcagcaat attcgcgatc acctgctcca
     8941 tcatcgggca gttctcgccc cagaagctga caaaatagtc ctgcatatca tggattaaca
     9001 acgcggcacg ttgcagttca aaggcccagt caactttatt ctgcggaata tcgtgagact
     9061 ccggcagtgc gtaagcctgt aattttggaa tagccatcgt gttctctcct tcaggctgat
     9121 gcgcgtgacg ccagccactg acttaattgt tttttatcga ctttcccgac cgccgtcagc
     9181 ggaagtgaat ctacacactc cacgcgatcc ggtaatttaa attcggcaat accctgttca
     9241 cgcaggaaac gacgcacctg caccgcgcgc agcggctctt ttaccaccag ataagcacag
     9301 cttttttcgc ccatcagctc atcttccatg ctcaccagtg cggcatagat caccgccgga
     9361 tggcgcagca gcaggttttc gatctcttcg gcagcgatct tctcgccgcc acggttgatc
     9421 tgatctttct cgcgcccctg cacggtgatg taaccctctg gatcaataga gatcagatcg
     9481 ccggaacagt aaaaaccgtt ggcatcaaag gcgctggcat tgtgctgcgg acttttgtaa
     9541 taaccgcgga aggtgtacgg cccgcgcgtc atcaggcgtc cgacttcccc ttgcggcagt
     9601 ggatttcctt cggcatcggc aacccacact tcatcgtccg gacacattgg gtaaccctgg
     9661 gtatggataa ttttctccgc gctatcatca agacgggtgt agttcaccag cccttccgcc
     9721 atgccaaaca cctgctgcaa ctggcagcca atctcagcgg gaatacgcgc cgcaagggtg
     9781 gcagaaagac gtgcgccgcc gacctgtaac agtttcagcg aggcaagctg cgcccggctt
     9841 tcgccttcag ccagcgcctg caaccacagg ctgactgccg gcggcaccag cgcggtgacg
     9901 ttcacctgat gtttttcaat caatgggaag caaagcgtag cgctgggatc ggccgccaga
     9961 acaaccgttc ctccggcaag aaagacgccc agcgatcctg gcgaactcat ggcgtagtta
    10021 tgagccgccg ggatcgcgca caggtagcgc gtctgttgtg tgaactgaca aatctcgacg
    10081 ctacgacgca cgctgtagta gtagtcgtta tgagtgcgcg ggatcagctt cggtgtaccg
    10141 gtggtgccgc cggaaagctg gaaataggcc acttcatcag caggtgatgc cgtggcagta
    10201 aaaccgtctg ccggatggtt aatcgcatcc tgcaagttat gctcaccgct gtcgttgtgc
    10261 agttgcacca cgcgaatgga ggaatgttct gtgacgaaag tattgaggaa atcatccccg
    10321 ctaaacagcg catgttggcg atcggcaatc agcaatgcgg gttcaatctg gctggcatag
    10381 gcgttcagtt cactacgctg atggctgaac aacgccagca ccggcgcaac gcccagtttc
    10441 agcagcgcga aaaaggtaat atacaattca gcgacgttac ccagttgtac cagcgcggtt
    10501 tcaccaggtt taatgccctg acggcgtaaa gtacacgcga ggttatccgc cgcctgattc
    10561 agctcccggt aactcaactg tagctcgccg tcgataaccg cgatgctgtc actcgcagcg
    10621 tggcgagtca gaatgtcggt cagcggcaaa tcctgccagt agcctttttc ccgatagcga
    10681 cgggcaaact cttccggcca gcgggtgaat ggaatgctca tcctcgctcc ttaatgcaat
    10741 ccaaaaacat tcaacatggt agaaagtttg acgcctgttt cgcgccactc acccaacggt
    10801 gacgacgcaa gcacaatccc cgctccggca aacagacgca cctgattttc ccgcagcttc
    10861 gcgcagcgga tggtcaccac ccattcgccg ttaccttcgc tgtcacacca acccacaatg
    10921 ccgccaaaca gttcgcggtc gaacggctcc agttcagcaa taacctgagt cgcggcctga
    10981 tgcgggaaac cgctcagcgc gggggtcgga tgcagcagac aggccagagt cagtgcgttt
    11041 tcttgcgaat tcgctttacc ttcaaaggga gttgcgagat gccacagcgt cggcgtggta
    11101 atcaattgtg gagaggaggg aacgtgtaac tcactactgc gttcgcgcag tacctctttc
    11161 atcgcctgag tcaccagttc atgttcatgg cgatcttttt ctgacgccag cagacgatta
    11221 cccgcttcgc gatcgagcac ttcatccggc tgacgacgcg cggaaccggc taacggaatg
    11281 gagctaaaac gctcgccgtc tttacgtagc agcagttccg ggctggcccc cagcaggacg
    11341 ccaccatcag ccagcgggac atggaagttg taactaaccg ggttttgcgc aatcaaccgt
    11401 tccagcaata cgccactatc aatggcggcg tcagtggtga tatcaatcaa ccgtgacaac
    11461 accactttgt cgacctgcgg cgtggcggta agtgcggcag cgcgggcaac catctgttca
    11521 aacgtggttt gttccggaat tgcctggcgt tccaccacat tcagcgactg gctgcgggtg
    11581 aaacggcggg cggaagcttg tttttcctga cgggagaacg actgccagga ttcaggaata
    11641 tacaacgacg aaggctgacg tggatcgaag ggaatcgccc cgaccatcac cggatttttg
    11701 atgccctgca ctttggcatc ggcaaacagc gcggcgagtt tttgctggaa gggactgtcg
    11761 ggcgaatccc cgttcacagc cggttcatcg aagcgggcga aacatcctga cgtcgtaaaa
    11821 ctgcggtacg gcgacataaa gaaaaagcga ttgggcgcaa gtgttgccat ggtctgctgt
    11881 acttcctcag ccagtgacgt atccatatca tcctccacaa aatgataaag gctttaataa
    11941 tgattatcat ttatattttc ggtcgctaac ctaaaagcaa acgcgcacta tgtcaactct
    12001 tgaggtaacg caatctgcaa ctcggggttg catctggttg tggttacaat gaaaatgaga
    12061 agcattattg atggattcgc ataagcgcaa tgtgatgtcc tgcgccgttc ttccccctct
    12121 cccttccagg gagagggctg gggtgagggt caatattcgc accagtgctg gctgctccca
    12181 tcaccctaac cctctcccca aaggggcgag ggtacggata gtgcgctttg tcgaatttgt
    12241 cattacgccc ttaaccttat taataacagg aagctgattt gtgagactcg ccccgctcta
    12301 ccgcaacgcc cttctattaa caggactttt gctttccgga atagccgcag ttcaggccgc
    12361 cgactggccg cgtcagatta ctgacagccg gggcactcat accctggaaa gccagccgca
    12421 gcgtattgtt tccaccagcg tcaccctgac cggctcactg ctggcgattg atgctccggt
    12481 gattgccagc ggcgcgacca cgccaaataa ccgcgtcgcg gatgaccagg gctttttacg
    12541 ccagtggagc aaggtggcga aagaacgcaa actacaacgg ctctatatcg gcgaaccgag
    12601 cgccgaagcc gttgccgcgc aaatgccgga tctgatttta attagcgcaa ccggcgggga
    12661 ttcggcgctg gcgctgtatg atcagctttc caccatcgcc ccgacattaa tcatcaatta
    12721 cgacgacaaa agctggcagt cgctgttaac gcagcttggc gaaattaccg ggcatgagaa
    12781 acaagcggca gagcggattg cacagtttga taagcaactg gcagcggcga aagagcaaat
    12841 caaattaccg ccgcagccgg tcactgctat tgtctatacc gccgctgcac acagtgccaa
    12901 tctctggacg ccggaatcag cacaagggca gatgctggaa caactcggct ttacgctggc
    12961 gaaattgccc acagggttaa acgccagcca aagccagggc aaacgccatg acatcattca
    13021 gcttagtgga gaaaatctgg ctgcagggtt aaatggcgag tcactattcc tgttcgccgg
    13081 tgatcagaaa gacgccgatg cgatttatgc caatccactg ctcgcgcacc tgcccgcagt
    13141 acaaaacaag caggtttatg cgctgggaac cgagacgttc cgcctggatt actacagcgc
    13201 catgcaagtg ctggaacgac ttaaggcgct gttttaagca ttaactgtcg gacgctgtca
    13261 cctgcggcgg cgtctggcga aaacgtcgca actccaccag caccagcaat aacaacacgc
    13321 cgataatcaa caaaccaaaa ccgctcgcgc ttgcggaagc aaccggtgtc atcatcgctc
    13381 ccagaccgcc taacagcgcc gcgcctatgg catcgcccgt cacgttttgt gccgtccaca
    13441 aaccgttgat tcgccctaac attgcttccg gcgtttgcgt ttgcagcatt gtatattgca
    13501 gcaacgaact gaccgcactc agccagccga acagcgccag acaaaccacg cctaaaatcc
    13561 acatcggcat caggccaaat aaaccaatgg cgaggaacga tcccaacgtg gagagcaaca
    13621 tcaacaatcc tggtcgcgcg ctatgcgcca gtttcccact ggttaatgcg ccaatagccg
    13681 caccgagcgg gatcgccgca tagagaaaac caatctctgc cgccgacatc tgccagttgt
    13741 cagccagcgc cggatacagt acccgcaccg cgctcgccat cgtcaataaa ccacccagca
    13801 acgcaatccc gcccaccagc gggctggcga gcagaaaacg aaatcctgcc agtaatgatt
    13861 tcaacggatg ctcgcgcggc tgcggtggcg gtggcaacgc cggaaggctt aacaacggta
    13921 gcaaggtaat aaacgtgccc gccgccgcca gcccgtagtt ccaggctacg ccaccggtcg
    13981 ccagcaataa accgccaatc atgggcgaaa tcaccgaccc cagacgcacg gtcaacatgg
    14041 tgatcgcccc ggcctgcatt aagttttcac gccctaccag tgctggtgtc gccgccaata
    14101 gcgccgtaac gccaagcgat gcgaaaaaac catcccataa accaagtaaa tagattgcca
    14161 gcaatgacgg ctccggcagc agtgcattaa ggcacagtcc aatgaagcca atgccacagg
    14221 tgccgcgcgc cagcaaaatc acttttttgc gctcatagcg atccgccagc acaccgccaa
    14281 ccatcaggcc aacaaacatc gcgccgccgg tcagcgtcac cgaaagcccc accagccagg
    14341 tagaatgcgt catcatctgg atctgcaccg gcaccgcgac gccgagcaaa cccagagaca
    14401 caattgagat aaaacgagcg aggaatactg cgcgaaacgc cgggtgcgtt ttcaacaggc
    14461 tgaggttaag cagccaggat tgtttattca ttacaatgcc ttgccatcaa atattttaaa
    14521 tacctattct taggctgcac atgctaacat atccaaataa gatcgataac gataattaat
    14581 ttcattatca tggaagttcg tatgtctggt tctgttgccg tgacacgcgc cattgccgtg
    14641 cccggattgc tgttattact gattatcgcg acggcattaa gcctgctcat tggggcaaaa
    14701 tcactccccg cttccgtagt gctggaggcc ttctccggca cctgccagag cgccgactgc
    14761 accatcgtgc tcgacgcgcg actgccgcgc acccttgccg gtttactggc aggcggcgcg
    14821 cttggccttg ccggggcatt gatgcaaacc ctcacccgaa atccacttgc cgaccccggc
    14881 ttgcttggcg tgaacgccgg agccagcttt gccattgtgc tgggtgcggc gctgtttggt
    14941 tactcatccg cgcaggaaca actggcgatg gccttcgccg gggcgctggt ggcctcattg
    15001 attgttgcct ttaccggcag tcagggcggc gggcagttaa gtccggtgcg tttaaccctg
    15061 gcgggcgtgg cgctggcggc agtgctggaa gggctgacca gcggcatcgc cctgcttaat
    15121 cctgacgtct acgatcaact gcgtttctgg caagccggtt cgctggatat tcgcaatcta
    15181 cataccttaa aagtggtgct gatcccggtg ctaatcgccg gagcaaccgc gctattactg
    15241 agccgcgcgc tgaacagttt aagcctcggc agcgacaccg cgacggcgct gggcagtcgc
    15301 gtggcgcgca cacagttaat tggtctgctg gcgattaccg tgctttgtgg tagtgcgaca
    15361 gcggtagttg gcccgattgc ctttattggc ctgatgatgc cgcatatggc gcgttggctg
    15421 gtgggtgccg atcatcgctg gtcgctgccc gtaacgctgc ttgctacccc tgccctgctg
    15481 ctgtttgccg atatcatcgg gcgggtgatt gtgcccggtg aactgcgcgt ttctgtggtc
    15541 agtgccttta ttggtgcacc ggtgctgatc ttcctcgtgc gacgtaaaac gcgaggtggt
    15601 gcatgattta cgtctctcgc cgattactca tcacctgttt gctgcttatt tccgcctgtg
    15661 tggttgcagg tatctgggga ttacgcagcg gtgccgtcac gctggaaacc tcgcaggtat
    15721 tcgccgcgct gatgggcgat gcgccgcgca gtatgacgat ggtggtcacc gaatggcgtt
    15781 taccacgcgt gctgatggcg ctgttgattg gcgcggcact gggcgtcagc ggtgcgattt
    15841 ttcagtcgct gatgcgtaac ccgctcggca gccctgacgt aatgggcttt aacaccgggg
    15901 cgtggagcgg cgtgctggtg gcgatggtgc tgtttggtca ggacctgacg gctatcgcgc
    15961 tggcagcaat ggtgggcggc attgtcactt ctctgttggt ctggctgctc gcctggcgta
    16021 acggtatcga cacctttcgg ttgattatta tcggcatcgg cgttcgcgcc atgctggtgg
    16081 cctttaatac ttggctgttg ctgaaagcgt ctttagaaac ggcgctaaca gcgggtttgt
    16141 ggaatgccgg atcgctcaat ggcctgacgt gggcaaaaac ttcgccttcc gcacccatca
    16201 ttatattgat gctcattgcc gccgccttgc tggtgcggcg aatgcgcttg ctggagatgg
    16261 gcgatgatac cgcctgcgcg ctgggcgtca gcgtcgagcg ttcgcgtctg ttaatgatgc
    16321 tggttgcagt ggtgcttacc gctgcggcaa cggcgcttgc cgggccgatt tcctttattg
    16381 ctttagtcgc gccgcacatt gcccgacgca ttagcggcac cgctcgctgg gggctgaccc
    16441 aggcggcgct gtgcggagcg ctgttactgc tggcggccga tctctgcgcc caacaactgt
    16501 ttatgccgta tcaacttccg gttggcgtcg ttaccgtcag cctcggcggt atttacctta
    16561 tcgtcttgtt aattcaggag tctcgcaaaa aatgaccgaa tcagtagccc gtttgcgcgg
    16621 cgaacagtta accctgggat atggcaaata taccgttgcg gaaaatctga ctgtagaaat
    16681 tcctgatggt cacttcacgg caattatcgg accaaatggc tgtggtaaat ccacgttact
    16741 gcgtacctta agccgcctga tgacgcctgc tcacgggcat gtctggctgg atggcgagca
    16801 cattaaacat tacgccagta aagaagttgc acgccggatt ggtctgttgg cgcaaaatgc
    16861 taccacgccg ggcgatatca ccgtgcagga gctggtggcg cgtggacgtt atccgcatca
    16921 accgctgttt acccactggc gcaaagagga tgaagaagcg gtaacgaaag cgatgcaggc
    16981 cacgggaata actcatctgg cagatcaaag cgtggatacc ctttctggcg gacaacgcca
    17041 gcgagcgtgg atcgcgatgg tgctggccca ggaaacggca attatgctgc tcgacgaacc
    17101 cacgacctgg ctggatatca gtcatcagat tgatctactg gaactgttaa gcgaactgaa
    17161 ccgcgagaaa ggctataccc tggcggcggt gctgcacgat cttaatcagg cctgtcgtta
    17221 cgccagccat ttgattgcat tgcgggaagg gaaaattgtt gctcagggag cgccgaagga
    17281 gattgtcact gctgaactga ttgagcgcat ttatggtctg cgatgcatga tcattgacga
    17341 tccggtagcc ggaacgccgc ttgtggtgcc gctcgaacga acggcacctt caaccgcaaa
    17401 tagttaaact aagtggtctg ccatcatggc atcctgtttt ctggatgcca tcgcatagcg
    17461 caataacacg ctaccacaag ccaccatccc gccgatcagc gcggaaagga tcacaataat
    17521 cgccttaccc ggaccgtctt ttttcactgg caatgacggg cttaactgat atttaaacgg
    17581 cgtaaaattc acatcgttga catttgcttt tgttaattgc tcgacaagat actgccgatt
    17641 acgtaattca ccgttcagtt ccgcaacgtc agtaaccgct ttttcaattt ccagtttgcg
    17701 ttcaataccg tctgcgccga gagaaataga gaaatcgggg tcatctttaa cggcctgacc
    17761 attactgtat acgggctttt taattcctgc cgcgttggca atgtcgagtg aataattgag
    17821 gcgctgaatg tttgcatcaa gttgattttt cgttttaatg cgatcctgag ccagtttttc
    17881 tttttcaaac tgggttttga tctccagttt attacggacg ttttctatcg actctttcac
    17941 caccaacgca gagatataat cgatataccc gctcaaaacg gtctgcgcct ctttactggt
    18001 tggcgcggta aaacttagcg tccaggaggt atacagtgac ggttcatctt ttttcttact
    18061 ggcattgtca tcaaccgctt tcattttttc gctcaatgca acaatagcgc gatgcaaatc
    18121 cagttcatcg attttcgcct cttttaattg gtccatcaca taaggtgatg aacgcaggta
    18181 ctcttccagc aagctaacag actgaaactt cttgataaac aggttaaatg caccgccacg
    18241 gtcgatatta acatcgagat caagaacgcg gagtttggta aaggttttct ccaggtcctg
    18301 ccactgaatg gcttcggcgg gagtaataac ggctgagctg gtccattttt gcggcaggat
    18361 gaaagagatc agtaaacctg cgcaggcaaa tgcaaaaacg accgccatga ccgttttttt
    18421 ggcccgccat aaaacttcga ttagattaag taaatcaatt tcattactac tgggcgacgc
    18481 cagaggatac tcggaaaaat gagcgtcact tccctgttta atattcagtg atgacatggg
    18541 tataacctga aaatgagtcc aataggggga aatctccacg gcgctcactt taccagttgg
    18601 tgagaattat ccgaagctga agtttgtaaa ttccttccac tgaatgcaac attcactcta
    18661 tttaagagaa atattcatca aacctataaa tcaaacaaat aaaaaaccca taattatttt
    18721 ctgtaattat gggtttatag ataatattaa tttacctgtt tagcgttgcg cgaataatcg
    18781 gcccaatttt tacaaacgcc cctggagaga taatatccac atgcgcacaa tcctgacgat
    18841 agatatccag ctcggcaatc cagggcgacc aagcgcgttc ggggctcata ccttcctgaa
    18901 gcgtgcgttc agcaacaaac agcgtcgctt taccatcaaa cggtacgcta tgagcagtcg
    18961 tcagcagacg cacagcatca gcgtagttgc cttcaatggt ggtaaacaac tccgttgaag
    19021 tacttccctg ctgtgccgcc aggaaggcct cgcgttcgcg gttaatctcc gccagcactt
    19081 ccgggtccag accattagcc tctttttcct gccagttttg cgtttctggc ggccaggtat
    19141 ccagcaaacc aagaaatgcc acctgttcgc cacgggcacg cagtcgcgcc gcaatgccct
    19201 gcgccagcgt accgccaagg gaatacccca gcaggtaata agggccgtgc ggttgttgtt
    19261 caagtaacgt tgccagatgc gcttcacaga cttcatccag atttgccgcg gtatgcatgg
    19321 ggccattggg gcgcggcgac tgaatgccga taatcgacca ttgtggatcg atataacgcg
    19381 agagcacgct aaactgccag gcaaaaccgg atgccggatg gaaacaaaac agcgtcgggc
    19441 cattaccttc acgcaacggc agaatggttt cgaatcccat acgccgggtg ctgtcttctt
    19501 cagcatcaat aatcgttgcc agtttagcga cggttgacgc gaccatcact tgccccggcg
    19561 tcacctggcg ggcaacctgc cgacttaact gcgctgccag tttcattgcc agtagcgaat
    19621 gaccaccaag cgcaaagaaa tcagcatcgg catcctgcac gtcacaaccc agcaacgacg
    19681 cgaatgccgc ggcaataatc gtttcactgc ccgcttttgg cgcacgcccc ggcgcttgtg
    19741 ccttcagttc aggcaacggt aaggctttgc gatccagctt gccgttggcg ctaagaggta
    19801 acagtggaag ttgcagcaga accacgggca ccatatgtgg tggcaatgtt tcgcgaagct
    19861 gcgcctgtaa tgcgctggta tccaacggca gacctgattg cgacaccaga tagcccacca
    19921 actgacgcgc atcaccaccg gtggctgccg cctggttaat cacacaggcg tgggtaacgg
    19981 cttgttcgac atccggcagc gcctgcatca cgcgatcgat ttcgcccagt tcaatacgct
    20041 gcccgcgaat ttttagctga tcgtcactgc gcccgaggta ctccaccgcg ccgttatcca
    20101 gccagcgggc aacgtctccg gtacggtaca tccgttcacc agggacaaaa ggatcggcaa
    20161 taaagcggct ggcggtcaga tcgggtcgtc caagataccc ctgcgccagt tgaatgccgg
    20221 tgagatagag atcacccgcc acacccggcg gcaccggatg catcatcgca tcaagaatac
    20281 gcagacccgt attccatacc ggataaccaa tcggcacact gctgccgcgc acctgtgcca
    20341 gttcctcgcc aaaagccgga taccagctga catctaccgc cgcttccgtc gggccatata
    20401 gattatgcaa cggcacgctg gttaactgtt gccattcgcg gcataaatca gccggtaagg
    20461 cctcaccact acagaaaacc tgtttcaacg tcgcgcaact ctggcgagcg gtttgcggcg
    20521 tcagcgatgc aacaaatgct gccagcatcg acggcacaaa gtgcgtggtc gttacgccat
    20581 attcggcaaa gaattgctgc atagcgagcg ggtcgcggtg cgcttccggt tcagccatca
    20641 ccagtttagc cccggcaata aacggccaga aaaactccca caccgagaca tcaaaactgc
    20701 acggcgtttt ttgggcaacg acatcttcgc ctgtaagcag ataatgattt tgcatccaca
    20761 acaggcggtt aacgatagcc gtctgcccga ccattacccc tttcggcctg ccggtggagc
    20821 cagaggtaaa gatgatataa gccgtgtgat gcggttgtga aagttgcagc ggcgcactgc
    20881 cctgcggttt aagcggggcg ttatagcaaa ggttggttaa atctggaaca tcggcaaagc
    20941 gcggcagttg atcgtcggtg gtgattaaca gcgacggacg cgcatcttcc agcatcattt
    21001 tcaggcgatc gtccggatag ccggtatcca gcggtagcca ggccgcgcca gcttcaacta
    21061 tcgcatgtag cgccagggtc aaaaagaccg agcgcggtag cgccaccgcc acgctgtcgc
    21121 ccggtttaac gccacgctca cgcagcagat tcgccagcgc caccacctgc tcgcgcattt
    21181 cccgatagct gaactggtaa cgcgcatctg ccagcgccgg agcatccggt gtttttgccg
    21241 cttgttctgc caccagcgcg ctcagcgtgg tttctggaat ctcaacctga gtggcgttga
    21301 tctgcgccag ctgcgcatac tcacctggca gcataatatc gacatcgccg cacaacagcg
    21361 ccggatccgc ggcgaactgg gcaatcagca ttttcaggcg ttcagcatgc tggattaacg
    21421 ttggctcatc gtaacgctgt ttattggcga ggatctcaat actcaaatca ccgtgttcat
    21481 ccgggaacag ggccagttca aggtcattaa ccggaccggt tgccaaggta tgggtttgcg
    21541 cctgaacacc aggaatatcc agttggtaat caaatacctt gatattgagt accggaccaa
    21601 acagcggttc atcacctgcc gctcgcccgc tgtcacggac aatttgttcg gcatcgtaac
    21661 gttgatgacg acgcattttt ttcagttgtg ctgccagtcg ggttgccagt tccggcagcg
    21721 tttcttgcgc cgcaatgtga atacccaacg gcaaaacgtt gagcacgggt ccggtagccg
    21781 tcagcgccgc cgagcccagt cgacgcataa agataaatcc agcggcgtag tccatgcgat
    21841 tgcacaatcg ccccagccac aaagccgtca gcgcaagggc taaatcggta cgctgcacac
    21901 ctgaaagttg cgtagccaac tggcggaatt ccccgtcggt aaattccatt ttcagacgta
    21961 aaatatctgc cgaagcgctg cgccccggta aaggtgccgg agaaagtgac gcgggcggcg
    22021 gcagttgacg acgctgttct gcccagaatg ccgcatcacg ctgccaggct tcgctttcgc
    22081 ggtattgctg gtactcttcc actacatcag cgaaaggcgt aaacggcgaa gcaggcgttg
    22141 gttcaccacg cagccatgtg cagtaaatat tggcgatctg gcgggtaatg gccgggaaac
    22201 tgaagccatc gaccagcaaa tggtgataac gctgatacca gtaccagcgg ttatccgcca
    22261 cctgtatcag ctgatgaaag accagtggtt taccgctatc gacgcgcaga tcttgttgca
    22321 aatccgcctg cattaatgcc tgcgcagtac cgtgcggatc aatgttggtt cgcaggtcga
    22381 taatttctgg cagttcgaac gtcagcgcat catcgaccca ctgccagact tcgccgttat
    22441 cttccgtaaa acgcatccgc agcgtatctg cttgcgctag tccgtcaacc accgcgcggg
    22501 ccagtaatgg cgcatcaacc tctccggtta actcaacgta atgcgctacg ctccaggcgg
    22561 agggtaaatc cgacagtttt tctgccatcc agatgccggg ctgtgcggcg accaaaggta
    22621 aatgctggct cattgcgcct cctgcaactg ggtgaaattc gccggtgtca gagtacgcca
    22681 gtgggcttca agccactgct ggcaggactc ttgtgactgc ggctgacaca caatgtccca
    22741 gcctgccggt aaggcgcatt gttgcggcca cagactgaat tgcccctgcg cattgcgcaa
    22801 aatgtaaaac gctccctgtg gatcatcgaa gggattactg aatgccatat tcaactcctg
    22861 tcatggaaaa gtggttgcca gaggtcgatt agcccctgca tcaagccacc gcgccaacaa
    22921 agcgcatcat gtccgccgtc aacctgacgc cagaaaatgg attcttttat ggggtgtaat
    22981 tgcgcataca gcgcctgatt ggcccgcata atcatcggct cgcgaatacc cgcttccagc
    23041 acaatgcgca gaccttcggc gctaacttca ccagctttaa gcttttcaag taacacgccc
    23101 tcttgctgcc cgccccgatg cggccaccag tacgatcctg actggcttaa tacacaacca
    23161 aaacgttcag gccagtgcag tccggcatac agcgcggaaa gtccaccaaa actctgcccg
    23221 gcaaccacgg tgcgatcggc gcgatcgcta aaaggggcaa tagctttcac caggggtaat
    23281 aactcttgct gtactgcgag ccagaaatcc gcattacacg gcagttcgtg agcgcggtgc
    23341 gtggtgtcga tagcgtcgat caacacatac acggcgggag gaagttgctg acgatgggtc
    23401 agcgaagtca gcactggcca gacgggcata ctttgcgccc aaaattcgcc atcgagcaaa
    23461 actgccagcg ggcgttcttc tgctgttgca tcgccggtgg taaaaatcca tacacgacgt
    23521 gaatttttca accgttcact tttccagata atttctttgg ctggtatttc tggcgcttgc
    23581 ggacaatccc atcccggttg cagaggcgct tgcggcattt cgagtgcaga aacagcgtgc
    23641 cctcgcccgc ctttccagct ttgtggattc agcggatcgg ctattgcctg gggtaatagt
    23701 tttcgccagc cttcacgtaa ttcgaggcga tcgggggatg gtgcagaaaa aatgtcatcg
    23761 cgttcggtgg gaataaagca gtagctgccg cgccagttgg cattgagttg tgtcgtccac
    23821 tgccagacat cagtacctgc aatgcgctgc atcgactggg gctggctgtt ctgatgatga
    23881 tcggtcacac cagtgatgta gacccatacg cgctttatcg gcgagtattc ttcagaacct
    23941 tggggctcac gccaccagaa agtgacctca aacatttcgt cattcagacg ctgccattcc
    24001 gggccatgtt tcgactgcca ccagctctca cttcctactt ttaacgccgt cacaccataa
    24061 ccccatgttt actgtgcaat ttttcattga ttgcagaaat atattgataa tattattgat
    24121 aactatttgc atttgcaata gcgtaatggc gcgccgtggg aagcgcggac attaattaac
    24181 caactgcact gcgtgtcttt caggatcaaa ggttttcgcg gtagcgggat gcgtcgtgtt
    24241 gaggacgacc atgcccgaca gttgcaattc gtggcaaaaa tgcaggaata aaacaatgaa
    24301 caagaagatt cattccctgg ccttgttggt caatctgggg atttatgggg tagcgcaggc
    24361 acaagagccg accgatactc ctgtttcaca tgacgatacc attgtcgtta ccgccgccga
    24421 gcagaacttg caggcacctg gcgtttcgac catcaccgca gatgaaatcc gcaaaaaccc
    24481 ggttgcccgc gatgtgtcgg agatcatccg taccatgcca ggcgttaacc tgaccggtaa
    24541 ctccaccagt ggtcagcgtg ggaataaccg acagattgat attcgcggta tgggtccgga
    24601 aaacacgctg attttaattg acggcaaacc ggtcagcagc cgtaactcgg tgcgtcaggg
    24661 ctggcgtggc gagcgcgata cccgtggtga tacttcctgg gtgccgcctg aaatgattga
    24721 acgtattgaa gttctgcgtg gtccggctgc agcgcgttat ggcaacggcg cggcgggtgg
    24781 cgtagttaac atcattacca aaaaaggcag cggtgagtgg cacggttcct gggacgctta
    24841 tttcaatgcg ccagaacata aagaggaagg tgccactaaa cgcaccaact tcagtctgac
    24901 cggcccgctg ggcgacgaat tcagcttccg cttgtatggc aacctcgaca aaacccaggc
    24961 tgacgcgtgg gatatcaacc agggccatca gtccgcgcgt gccggaacgt atgccacgac
    25021 gttaccagcc gggcgcgaag gggtgatcaa caaagatatt aatggcgtgg tgcgctggga
    25081 cttcgcgcct ctgcaatcac tcgaactgga agcgggttac agccgccagg gtaacctgta
    25141 tgcgggcgat acccagaaca ccaactctga cgcttacact cgctcgaaat atggcgatga
    25201 aaccaaccgc ctgtatcgcc agaactactc gctgacctgg aacggtggct gggataacgg
    25261 agtgaccacc agcaactggg tgcagtatga acacacccgt aactcgcgta ttccggaagg
    25321 tctggcgggc ggtaccgaag ggaaatttaa cgaaaaagcg acacaggatt tcgtcgatat
    25381 cgatcttgat gacgtgatgc tgcacagcga agttaacctg ccgattgatt tcctcgttaa
    25441 ccaaacgctg acgctgggta cggagtggaa tcagcaacgg atgaaggact tgagttccaa
    25501 tacccaggcg ctgaccgggg cgaacaccgg cggcgctatt gatggtgtga gtgccaccga
    25561 ccgtagcccg tattcaaaag cagaaatttt ctcgctattt gccgaaaaca acatggagct
    25621 gactgacagc accatcgtaa cgccggggct gcgtttcgat catcacagta ttgtcggcaa
    25681 taactggagc ccggcgctga acatatcgca aggtttaggc gatgacttca cgctgaaaat
    25741 gggcattgcc cgcgcctata aagcgccgag cctgtaccag actaacccga actacattct
    25801 ctacagtaaa ggtcagggct gctatgccag cgcgggcggc tgctatctgc aaggtaacga
    25861 tgacctgaaa gcagaaacca gcatcaacaa agagattggt ctggagttca aacgcgacgg
    25921 ctggctggcg ggcgtcacct ggttccgtaa cgattatcgc aataagattg aagcgggcta
    25981 tgtggctgta gggcaaaacg cagtcggcac cgatctctat cagtgggata acgtgccgaa
    26041 agcagtggtt gaaggtctgg aaggatcgtt aaacgtacca gttagcgaaa cggtgatgtg
    26101 gaccaataac atcacttata tgctgaagag tgaaaacaaa accacgggcg accgtttgtc
    26161 gatcatcccg gagtacacgt tgaactcaac gctgagctgg caggcgcggg aagatttgtc
    26221 gatgcaaacg accttcacct ggtacggtaa gcagcagccg aaaaaataca actacaaagg
    26281 tcagccagcg gttggcccgg aaaccaaaga aatcagtcct tacagcattg tgggtctgag
    26341 cgcgacctgg gatgtgacga agaatgtcag tctgaccggc ggcgtggaca acctgttcga
    26401 caaacgtttg tggcgtgcgg gtaatgccca gaccacgggc gatctggcag gggccaacta
    26461 tatcgcgggt gccggggcgt atacctataa cgagccggga cgtacgtggt acatgagcgt
    26521 aaacactcac ttctgatgct aacgtcagat tgttgataaa gtgcgcgtcg ttcatgccgg
    26581 atgtggcgtg aacgccttat ccggcctaca aaaatcttgc caattcaata tattgcagga
    26641 ccatgtaggc ctgataagcg tagcgcatca ggcagtttag cgtttgtcac cagtctctaa
    26701 tatggtcgac atgaaaacta cgcatacctc cctccccttt gccggacata cgctgcattt
    26761 tgttgagttc gatccggcga atttttgtga gcaggattta ctctggctgc cgcattacgc
    26821 gcaactacaa cacgctggac ggaagcgcaa agccgaacat ttagccgggc ggatcgctgc
    26881 aatttatgcg ctgcgggaat atggctataa atgtgtgccc gcaatcggcg agctacgcca
    26941 accggtgtgg cctgcggagg tatacggcag tattagccac tgcggggcta cggcattggc
    27001 cgtggtttcc cgtcaaccga ttggcgttga tatcgaagaa attttttctg cgcaaacagc
    27061 gaccgaattg acagacaaca ttattacacc agcagaacac gagcgactcg cagactgcgg
    27121 tttagccttt tctctggcgc tgacactggc attttccgcc aaagagagcg catttaaggc
    27181 aagtgagatc caaactgatg caggttttct ggactatcag ataattagct ggaataaaca
    27241 gcaggtcatc attcatcgtg agaatgagat gtttgctgtg cactggcaga taaaagaaaa
    27301 aatagtcata actctgtgcc aacacgatta attgacaaca tctggtacga ttcgcccgca
    27361 gccatcactg accgcgggcg aaagtgtaaa gcaggtgcct taccatcctg acctgacaac
    27421 cggatatgcg gggattgctc cccgcacaac ggctacttct tcggttcgta agcgagaaca
    27481 gccttaaact caatattacg ttcctttacg gtgaactcac acagcgaatc cccgaccaga
    27541 agcgtaaatc ccagcaccgt taaacacagc actatgactg ccgcaagggc atatttcgtc
    27601 agcatgttta tattgcctcc atgcgaagaa gggactacca tccaaattgt tcaggtttgg
    27661 gtaggtagcc ccagggttga tagaaatatc gtcctggggc ttcatctttc cagacctcag
    27721 agtaaacctg aaaccggaaa gcttcaggca cccgcagtta tcttaccctt gcttagcaaa
    27781 atagctgttt atttatacag taagtcctgt ttcggacttc tcactcccca tttgatcaac
    27841 tgaagctgaa cccgtttttt tctgcatgcc tcaatcgtca aaattcttcg ccaccagtca
    27901 gcactgccat aacttgtcac attgcaaaag tagccaaatt gcttctgtca taatcagttc
    27961 accatcacca aaaaaaagaa acattccaga acttccgcat ctcgcccagc cgctcccact
    28021 tctatactga atagaaaacg ccaacataag agaaacctat gccattaccc gattttcatg
    28081 tttctgaacc ttttacactc ggtattgaac tggaaatgca ggtggttaat ccgccgggct
    28141 atgacttaag ccaggactct tcaatactga ttgacgcggt taaaaataag atcacggccg
    28201 gagaggtaaa gcacgatatc accgaaagta tgctggagct ggcgacggat gtttgccgtg
    28261 atatcaacca ggctgccggg caattttcag cgatgcagaa agtcgtattg caggcagccg
    28321 cagaccatca tctggaaatt tgcggcggtg gcacacaccc gtttcagaaa tggcagcgac
    28381 aggaggtatg cgataacgag cgctatcaac gaacgctgga aaactttggt tatctcattc
    28441 aacaggtgac cgtttttggt cagcatgttc atgttggctg cgccagtggc gatgacgcca
    28501 tttatttgct gcacggcctg tcacggtttg tgccgcactt catcgccctt tccgccgcgt
    28561 cgccatatat gcaaggaacg gatacgcgtt ttgcctcctc acgaccgaat attttttccg
    28621 cctttcctga taatggcccg atgccgtggg tcagtaactg gcaacaattt gaagccctgt
    28681 ttcgctgtct gagttacacc acgatgatcg acagcattaa agatctgcac tgggatattc
    28741 gccccagtcc tcattttggc acggtggagg ttcgggtgat ggataccccg ttaaccctta
    28801 gccacgcggt aaatatggcg ggattaattc aggctaccgc ccactggtta ctgacagaac
    28861 gcccgttcaa acatcaggag aaagattacc tgctgtataa attcaaccgt ttccaggcct
    28921 gtcgctatgg gctggaaggc gtcattaccg atccgcacac tggcgatcgt cgaccgctaa
    28981 cggaagacac cttgcgattg ctggaaaaaa tcgccccttc cgcacataaa attggtgcat
    29041 cgagcgcgat tgaggccctg catcgccagg tcgtcagcgg tctgaatgaa gcgcagctga
    29101 tgcgcgattt cgtcgccgat ggtggctcgc tgattgggct ggtgaaaaag cattgtgaga
    29161 tctgggccgg tgactaagta gggaattgcc ttacagaaga atatccctga caatggtgtt
    29221 ttaacttaca tttaacaatg ttatgaaaca ccctttagaa accttgacta ccgcagcagg
    29281 cattttgctg atggctttcc tctcttgcct gctgctgccc acccccgcac tggggctgac
    29341 gctggcacaa aaactggtga ccatgttcca tctgatggat cttagccagc tttacacttt
    29401 attgttttgt ctgtggtttt tagtgctggg cgctattgag tattttgttc tgcgctttat
    29461 ctggcgacgc tggttctcgc tggcggatta aacgtcgatt atcgccgtac ttcgcggcat
    29521 actttgctta ttctccttcg cctaaaggaa cgtttatgga taagcaatca ctgcacgaaa
    29581 cggcgaaacg cctggccctt gagttaccct ttgtcgagct ttgctggcct tttggcccgg
    29641 agttcgatgt ttttaaaatt ggcggcaaaa tttttatgct gtcgtcggag ctgcgcggcg
    29701 tcccctttat caatctgaag tccgatccac aaaaatccct gttaaatcag cagatatacc
    29761 cgagcattaa gccagggtat cacatgaata aaaagcactg gatttcggtg tatcccggcg
    29821 aggaaatctc cgaagcgtta cttcgcgatc tgatcaacga ttcgtggaat ctggtggttg
    29881 atggtctggc taaacgcgat caaaaaagag tgcgtccagg ctaaagcgga aatctatagc
    29941 gcatttttct cgcttaccat ttctcgttga accttgtaat ctgctggcac gcaaaattac
    30001 tttcacatgg agtctttatg gatatcattt ctgtcgcctt aaagcgtcat tccactaagg
    30061 catttgatgc cagtaaaaaa cttaccccgg aacaggccga gcagatcaaa actctcctgc
    30121 aatacagccc atccagcacc aactcccagc cgtggcattt tattgttgcc agcacggaag
    30181 aaggtaaagc gcgtgttgcc aaatccgctg ccggtaatta tgtgttcaac gaacgtaaaa
    30241 tacttgatgc ctcgcacgtc gtggtgttct gtgcaaaaac cgcgatggac gatgcctggc
    30301 tgaagctggt tgttgaccag gaagatgctg atggccgctt tgccacgccg gaagcgaaag
    30361 ccgcgaacga taaaggtcgc aagttcttcg ctgatatgca ccgtaaagat ctgcatgatg
    30421 atgcagagtg gatggcaaaa caggtttatc tcaacgtcgg taacttcctg ctcggcgtgg
    30481 cggctctggg tctggacgcg gtacccatcg aaggttttga cgccgccatc ctcgatgcag
    30541 aatttggtct gaaagagaaa ggctacacca gtctggtggt tgttccggta ggtcatcaca
    30601 gcgttgaaga ttttaacgct acgctgccga aatctcgtct gccgcaaaac atcaccttaa
    30661 ccgaagtgta attctctctt gccgggcatc tgcccggcta tttcccctca gattctcctg
    30721 atttgcataa ccctgtttca gccgtcatca taggctgctg ttgtataaag gagacgttat
    30781 gcaggattta atatcccagg ttggggattt agcgggtatt gagatcgatc acaccacctc
    30841 gatggtgatg attttcggta ttatttttct gaccgccgtc gtggtgcata ttattttgca
    30901 ttgggtggta ctgcggacct tcgaaaaacg tgccatcgcc agttcacggc tttggttgca
    30961 aatcattacc cagaataaac tcttccaccg tttagctttt accctgcagg ggattatcgt
    31021 caatattcag gcggtattct ggctgcaaaa aggcaccgaa gcggcagata ttctgactac
    31081 ctgcgcgcag ttgtggatca tgatgtatgc gctgctttca gtcttctcgt tgctggatgt
    31141 tattttgaat ctggcgcaga aattcccggc ggcatctcag ttaccgctga aagggatatt
    31201 tcaggggatt aaactgatcg gcgcaatcct ggtcggcatt ttgatgatct cgctgctgat
    31261 tggtcagtcg ccagcgattc tgatcagcgg tcttggtgca atggctgccg tgctgatgtt
    31321 ggtatttaaa gatccgattc ttggtctggt ggcaggtatt cagctttccg cgaacgatat
    31381 gctgaaactg ggcgactggc tggagatgcc gaaatacggc gcggatggcg cggtgatcga
    31441 tattgggtta accaccgtca aagtgcgtaa ctgggacaat accattacca ctattcccac
    31501 ctggtctctg gtttctgact cctttaaaaa ctggagcggg atgtcagcat ctggcgggcg
    31561 acgtattaag cgcagtatca gtattgatgt caccagtatt cgttttcttg atgaagacga
    31621 aatgcaacgt ctgaataaag cgcacttgtt aaaaccttat ttaaccagcc gccatcagga
    31681 aattaatgag tggaatcgcc agcaaggttc tacggagtcg atattaaacc tgcgccgaat
    31741 gaccaatatt ggaacctttc gtgcctatct gaacgaatat ctgcgtaacc atccgcggat
    31801 tcgtaaagat atgaccttaa tggtacgcca actggctcca ggtgataacg gtttaccgct
    31861 cgagatctac gcgtttacca acaccgtggt gtggctggaa tatgaaagca ttcaggctga
    31921 tatattcgat cacatatttg cgattgtcga agagtttggt ctgcgacttc atcagtcgcc
    31981 agccggcaat gatattcgct ctctggcggg tgcatttaag cagtaattaa aaaaaccgct
    32041 ctcatcgaat ggatgagagc ggacatcgtt ttgtcggatg tagcgtgaat gccttatttc
    32101 cgacgcagcg ttttaaatgc cacaaacagg aatacaatcc acaccggcag caggatcgct
    32161 gacaagcgca tatcatccat cgtgcacatc agcagcaaaa tcatgccgag gaaggcaatg
    32221 caaagatagt tgccgaacgg ataaagcagc gccttaaact gtgtttcacg tccctgacgt
    32281 cgcatcgccg cgcgaaaacg cagatgcgcc aggcagatca taatccagtt caacagcagc
    32341 gttgctacca ccagcgccat cagcagacca aacgcttttt gcggcagcag atagttgatt
    32401 aacaccacca gcgaagtgat cgctccggaa agcatcagcg agttaatcgg cacgccgcga
    32461 tggctgacgc gagtcaaaaa cttcggcgca ttaccctgca cagaaaggcc aaacagcatg
    32521 cggctgttag agtaaacccc gctgttatac actgacagcg atgctaccag aatgacgaag
    32581 ttcagcgcag aagctacctc gttgctgtcg agattatgga aaatcatcac aaacgggcta
    32641 ctgttggatt tcacttccac ccacggatag agcgccagta aaaccaccag tgaacagatg
    32701 taaaacagca ggatgcgata caccacctga tttaccgctt ttggaatgct tttttccgga
    32761 tcgcgcgctt cagcggcagt aatcccaatc agctccagac cgccgaagga gaacataatt
    32821 accgccagcg acaaaatcag cccattccag ccggtggcga agaaaccacc gtagcgccag
    32881 aggttgtcga tactggcttt ctcgccgccg tgaccagaaa acagcagcca caggccaaag
    32941 ccgatcatac cgatgattgc cagcacttta atcaacgcaa accagaactc ggtttcgcca
    33001 tataagcgca cgttcaccag gttaacggcg ttgatgataa taaagaaggc ggcagcccaa
    33061 atccacgttg gaacatccgg gaaccagtac tgcatataga tgcccgcagc ggtcagctct
    33121 gccattccca ccagcacgaa cattacccag tagttccagc cagagaggaa gcccgcaaac
    33181 ggtccccagt atttataggc aaagtgggca aatgaaccgg ataccggctc ttcgaccacc
    33241 atctcgccga gctggcgcat aatcaggaaa gcgatgatcc cggcgacgcc gtagcccagc
    33301 aatacagccg gacccgccat ctgaatcgcc gggccaatgc caagaaacag accagtacca
    33361 attgcgccac ccagcgcaat cagttgaata tgacggttat gtaatccgcg atgaagcgtc
    33421 ggctcttgat ttgacgcagt atcttccgat acggttgacg cgtttttcac gcctttcccc
    33481 tgtgtgtctt ttttgttgag gggcaccttt taacatttag tgcccatcgt cgcaagacac
    33541 aatccacacg gttaaacggg gtatcctgct tttatttccg tacccgatgt cggtgcagcc
    33601 acatcagctt atacgccgcc gggataataa acagcgacag caaaggcgcg gtgatcatgc
    33661 cgccaatcat cggtgcggca atccggctca tcacctctga accagccccc gttccccaca
    33721 gaatcggcag cagaccggcg ataatcaccg ccaccgtcat cgctttcggg cgcacgcgca
    33781 gtaccgcgcc gtgatacaac gcctcatcca gcttctgctc actgaatgtt tgcggattat
    33841 tcaacgacgg ctcggcctct atggcgtgac gtaaatacat cagcatcacc acgccaaatt
    33901 cggcggcgcc cccggcaagg gcaataaaac cggtgcccgt tgccacagaa agatgaaagc
    33961 ccatccacca cagcaaccag atgccgccca ccagcgcaaa cggtacgctg ctgataatca
    34021 gcaacgcttc gcccacccga cggaatgcca gatacaacag cacgaagatg atcatcagtg
    34081 tcatcggcac catgagtttc agcttatggt tggcgcgctc cagtagctcg aactgcccgg
    34141 agaatgccac gctggtgccc ggttttaact gcactttttc aactatcgct ttttgcaaat
    34201 cgtgaaccac cgacaccatg tcacgatcgc gggcatcgat ataaatccag ctcgtcgggc
    34261 gcgcattctc ggttttcagc atcgacggtc cggtagagac tttaacgtcg gccacgtctg
    34321 ccagggtgat ttgctgcttc atcggcgtca ggatcggtag ctggcgcagt gcctgctttg
    34381 cggataacgc agattaatgg gataacgggc aatcccttcc accgtttcgc caaccatcgc
    34441 cccgcccacc gcagaagtca caaacaactg cacatccgcc accgtcatac cgtaacgcgc
    34501 ggctttttca cggttaatct caacgttgat atagcgccca ccttccagac gctcggcaag
    34561 cgcagaagcc acgcctggca ccgttcgcgc tacttcttca atttgctcag ccatcgcgtc
    34621 gatatccgcc agcacagtgc cggaaacttt aatgccgatg gggcttttaa tgccggttga
    34681 gagcatatcg atacggttac gaattggcgg cacccacaga ttcgccagcc ccggcagccg
    34741 cacggtgtta tccagttcct caatgatttt gtccatcgtc atgccaggcc gccactgatc
    34801 ctgcggctta agctggatgg tcgtttcaac catctcgagc ggagcagaat cggtggcggt
    34861 ttccgctttc ccggttttgc caaatacccg cgccacttca ggtacgctca taattagctt
    34921 gtcgtttttt tgcagcatac tcgccgcctc tgctgcggaa atccccggca gcgtcgatgg
    34981 catatacaac aagtcgcctt cattgatctg cggtaaaaat tccccgccaa ctttattgag
    35041 cggccagaga accgtcagca ccgaaagcgc tgccaccaac agcgtggttt tcggccagtg
    35101 cagtactttc agcaatagag gatgataaac acgaatcaaa aagcgattga gcgggttact
    35161 gctttccggc ggaattttgc cacggatcca gtaacccatc aggatcggga tcactacgat
    35221 cgccagcagc gccgcacccg ccatcgcata cgttttggtg aacgccagcg ggccaaacag
    35281 acgcccttct tgcccttcca gggtgaagat tgggataaac gacagcgtga taatcagcag
    35341 actgataaac agcgccggcc ccacttcaac agatgcatcg gtaatcacct gccagcgcgt
    35401 tttattatcc agcgtggcgt caggatgctg gtgctgccac tcttccagcc gtttatgcgc
    35461 attctcgatc atgacgatag cagcatcgac catcgccccg acggcaatcg caatgccacc
    35521 caacgacata atattggcat tcagtccctg gaagggcatg acaataaaag caatacacaa
    35581 ccccagcggc aacgaaataa tcgccaccag cgccgagcgc acatgccaga gaaacagcgc
    35641 acagactacc gccaccacaa taaactcttc cagcaacttg ccgctgaggt tgtcgatggc
    35701 gcggtcaatg agctggctgc gatcgtatgt tgtaactatc tccacgcctt ccggcagact
    35761 acttttcagc gtttccagtt tgtccttcac ggcggcgatc acttctcggg cgtttttacc
    35821 ggagcgcagg atcaccaccc cgcccgccac ttcgccttcg ccgttcagtt cggcaatgcc
    35881 ccggcgcatc tccgggccaa cctggacctt cgcaacatcg cgcagataaa cgggcacgcc
    35941 attttcactg gcttttaaaa cgatgtgatt aaagtcgtcg agcgtttgca gatagccgct
    36001 ggcgcgcacc atatattccg cttccgccag ttcgatcgac gaaccgcccg cttcctggtt
    36061 tgaagcatcc agcgcgcttt ttacttcggc gagactgatg ccatactgcg ccaggcgctg
    36121 gggatcgata accacctgat actctttcac cacaccgccc accgacgcca cttccgcaac
    36181 gtcagggatg gttttcagct catatttgag aaaccagtcc tgtaatgagc gtaaatcggc
    36241 cagatcgtgc ttaccgctgc gatccaccag tgcatattca tagatccagc caacacccgt
    36301 ggcatctggc cccagctcgg cgctgactcc cgcaggcagc ttaccctgta cctggttgag
    36361 gtactccagc acccgcgaac gcgcccagta cggatcggta ccatcttcga aaatgacata
    36421 cacataagag tcgccaaact gcgagaaacc acgcacagtc ttcgcgccag gcaccgacaa
    36481 catggtggtg gttagcggat aagtcacctg attttcaacg atttgcggtg cctgcccggg
    36541 atagctggtt ttaataatca cctgcacatc ggagagatcc ggcagcgcat ccactggcgt
    36601 attaatgatg gtccaggtgc cccagatgct aagaaacaac gcgcccatca gcaccaaaaa
    36661 acggttcgcc accgagcgac gaataatcca ttcaatcatt ggttattccc tcaatgcgca
    36721 tgggtagcac tttcagagcg catccgctcc agtgcgccag aaatattggc ttcggaatca
    36781 atcaggaaaa ggccgctgga aaccaccttt tcaccttccg ccagaccaga gcgtaatgcg
    36841 gtgacacctt gcgatgcctg gaaaacggca acgcgtttcg gtacaaagcg cccgtcggca
    36901 tcaatggtaa tcacccgctg ttcgctaccg gtatcaatca gcccttgtga cggaatgagc
    36961 agcatcggtt cgctggcggt attgagttgc aaccaggcgt tcatgcccgg ttttagcgcc
    37021 tcgtcggcgt tgtcgacttc cagacgcagc tgcagcgtgc gggtcgcggc atccacgccg
    37081 ggtagcagcg tccatttgcg gatggtgagt gttttatccg gtcgtgccgg aacggtgagc
    37141 gtaaactgcg aggcatcttt caccagccag gcgatagact ccgggatcgc agcagtgacc
    37201 cacaccgggt ccataccctg aattttcgct accacgttat ctttggcgat attcattccc
    37261 gcgcgcagat caaacgcggt gatcacgcca tcaatgggcg ctttgagcgt aaagcgagtc
    37321 tggatttttt gcgtggcgat caggcggcga atatccgcct ccggcattcc cgccagtcgc
    37381 agccgctcaa gaatgccttc agtctgggtc gccgtaccgc cggtttcgcg cagcagtaaa
    37441 tactcactct gcgcttccac ccagtcagga atggtcaggt cgagaagcgg tgtgcccttt
    37501 tgtactttat cgcccacggt aagcggatac accttgtcga taaacccggc agcgcgagcc
    37561 tgcacaatgg catactgata ctcgttgtaa ctgacattcg ccgggaaact ctgggcaaaa
    37621 gtcagcggtc cgcgcgtgac ggtagccgtt ttcaccccca gattctgcgt ctgagtcggg
    37681 tcaatgcgca caccagacgc agaactctct tcatcggcat atttcggcac cagatccata
    37741 tccataaacg gcgatttacc tggtttatcg aaccgcgtat tggggtacat cgggtcgtac
    37801 cagaataaga ttttacgttc tgc
//
LOCUS       DAJTMC010000017        37610 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.017, whole genome shotgun sequence.
ACCESSION   DAJTMC010000017 DAJTMC010000000
VERSION     DAJTMC010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37610
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.017"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            <1..1985
                     /locus_tag="OO130_000782"
     CDS             <1..1985
                     /locus_tag="OO130_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
                     /protein_id="HCR5364293.1"
                     /translation="VGAFLAGENGNSTTGDAIYMRGADTSNSIYIDGIRDIGSVSRDT
                     FNTEQVEVIKGPSGTDYGHSAPTGSINMISKQPRNDSGIDASASIGSAWFRRGTLDVN
                     QVIGDTTAVRLNVMGEKTHDAGRDKVKNERYGVAPSVAFGLGTANRLYLNYLHVTQHN
                     TPDGGIPTIGLPGYSAPSAGTAALNHSGKVDTHNFYGTDSDYDDSTTDTATMRFEHDI
                     NDNTTIRNTTRWSRVKQDYLMTAIMGGASNITQPTSDVNSWTWSRTANTKDVSNKILT
                     NQTNLTSTFYTGSIGHDVSTGVEFTRETQTNYGVNPVTLPAVNIYHPDSSIHPGGLTR
                     NGANANGQTDTFAIYAFDTLQITRDFELNGGIRLDNYHTEYDSATACGGSGRGAITCP
                     AGVAKGSPVTTVDTAKSGNLVNWKAGALYHLTENGNVYINYAVSQQPPGGNNFALAQS
                     GSGNSANRTDFKPQKANTSEIGTKWQVLDKRLLLTAALFRTDIENEVEQNDDGTYSQY
                     GKKRVEGYEISVAGNITPAWQVIGGYTQQKATIKNGKDVAQDGSSSLPYTPEHAFTLW
                     SQYQATDDISVGAGARYIGSMHKGSDGAVGTPAFTEGYWVADAKLGYRVNRNLDFQLN
                     VYNLFDTDYVASINKSGYRYHPGKPRTFLLTANMHF"
     gene            2027..2704
                     /gene="ybiX"
                     /locus_tag="OO130_000783"
     CDS             2027..2704
                     /gene="ybiX"
                     /locus_tag="OO130_000783"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HCR5364294.1"
                     /translation="MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDKNIQSLKSRYGENEEILSLLNLYHNL
                     LREWSEI"
     gene            2778..3044
                     /gene="ybiI"
                     /locus_tag="OO130_000784"
     CDS             2778..3044
                     /gene="ybiI"
                     /locus_tag="OO130_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HCR5364295.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
     gene            3309..3569
                     /locus_tag="OO130_000785"
     CDS             3309..3569
                     /locus_tag="OO130_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR5364296.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLTEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(3797..4882)
                     /gene="hcxB"
                     /locus_tag="OO130_000786"
     CDS             complement(3797..4882)
                     /gene="hcxB"
                     /locus_tag="OO130_000786"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HCR5364297.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGYLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCSAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMSEETLQAFCQQLAS"
     gene            complement(5023..5985)
                     /gene="ybiB"
                     /locus_tag="OO130_000787"
     CDS             complement(5023..5985)
                     /gene="ybiB"
                     /locus_tag="OO130_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HCR5364298.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDTAGSELLPQAKDPETTAQ
                     WIERCLAGSEPIPESLKIQMACCLEATGEAATISDGLARVNQAF"
     gene            complement(6013..8163)
                     /gene="dinG"
                     /locus_tag="OO130_000788"
     CDS             complement(6013..8163)
                     /gene="dinG"
                     /locus_tag="OO130_000788"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HCR5364299.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRFE
                     PSIDNEEQHIAEMAAFFREQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            8283..8765
                     /gene="ybiA"
                     /locus_tag="OO130_000789"
     CDS             8283..8765
                     /gene="ybiA"
                     /locus_tag="OO130_000789"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HCR5364300.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAAWPIKVDGKTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRNRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRALLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            complement(8997..10361)
                     /gene="rhlE"
                     /locus_tag="OO130_000790"
     CDS             complement(8997..10361)
                     /gene="rhlE"
                     /locus_tag="OO130_000790"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HCR5364301.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSSDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGGRGQGGGRGQQQPRRGEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRKP
                     AAAQ"
     gene            10590..11261
                     /gene="cecR"
                     /locus_tag="OO130_000791"
     CDS             10590..11261
                     /gene="cecR"
                     /locus_tag="OO130_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HCR5364302.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNTTTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            11264..12259
                     /gene="hlyD"
                     /locus_tag="OO130_000792"
     CDS             11264..12259
                     /gene="hlyD"
                     /locus_tag="OO130_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HCR5364303.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLKLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQVKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGNEAGHE"
     gene            12252..13988
                     /locus_tag="OO130_000793"
     CDS             12252..13988
                     /locus_tag="OO130_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5364304.1"
                     /translation="MNDAVITLNGLEKRFPGMGKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGNATMIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELASEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            13981..15114
                     /locus_tag="OO130_000794"
     CDS             13981..15114
                     /locus_tag="OO130_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5364305.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            15125..16231
                     /locus_tag="OO130_000795"
     CDS             15125..16231
                     /locus_tag="OO130_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5364306.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIATNYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(16193..16603)
                     /locus_tag="OO130_000796"
     CDS             complement(16193..16603)
                     /locus_tag="OO130_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HCR5364307.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            16736..17487
                     /locus_tag="OO130_000797"
                     /pseudo
     CDS             16736..17487
                     /locus_tag="OO130_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
     gene            17484..18725
                     /gene="clsB"
                     /locus_tag="OO130_000798"
     CDS             17484..18725
                     /gene="clsB"
                     /locus_tag="OO130_000798"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HCR5364308.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNKLTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWHPAHTPRLTQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            18725..19681
                     /locus_tag="OO130_000799"
                     /pseudo
     CDS             18725..19681
                     /locus_tag="OO130_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415309.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
     gene            complement(19717..20430)
                     /locus_tag="OO130_000800"
     CDS             complement(19717..20430)
                     /locus_tag="OO130_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCR5364309.1"
                     /translation="MESYSQNSNKLDFQHETRILNGIWLITALGLVATAGLAWGAKYL
                     EITATKYDSPTMYVAIGLLLVCMYGLSKDINKINAVIAGVIYLFLLSLVAIVVASLVP
                     VYAIIIVFSTAGAMFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIVNAALMSERP
                     IWVISCLVIVLWPGIISHGRNKLLELAGKCHSEELWSPVRCAFTGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
     gene            complement(20636..21340)
                     /locus_tag="OO130_000801"
     CDS             complement(20636..21340)
                     /locus_tag="OO130_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCR5364310.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            complement(21477..21929)
                     /gene="moaE"
                     /locus_tag="OO130_000802"
     CDS             complement(21477..21929)
                     /gene="moaE"
                     /locus_tag="OO130_000802"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HCR5364311.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFAGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            complement(21931..22176)
                     /gene="moaD"
                     /locus_tag="OO130_000803"
     CDS             complement(21931..22176)
                     /gene="moaD"
                     /locus_tag="OO130_000803"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HCR5364312.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADFPTVEALRQHMAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            complement(22169..22654)
                     /gene="moaC"
                     /locus_tag="OO130_000804"
     CDS             complement(22169..22654)
                     /gene="moaC"
                     /locus_tag="OO130_000804"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HCR5364313.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            complement(22657..23169)
                     /gene="moaB"
                     /locus_tag="OO130_000805"
     CDS             complement(22657..23169)
                     /gene="moaB"
                     /locus_tag="OO130_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HCR5364314.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(23191..24180)
                     /gene="moaA"
                     /locus_tag="OO130_000806"
     CDS             complement(23191..24180)
                     /gene="moaA"
                     /locus_tag="OO130_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HCR5364315.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(24168..24312)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            24577..25485
                     /gene="yvcK"
                     /locus_tag="OO130_000807"
     CDS             24577..25485
                     /gene="yvcK"
                     /locus_tag="OO130_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HCR5364316.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     gene            complement(25677..27698)
                     /gene="uvrB"
                     /locus_tag="OO130_000808"
     CDS             complement(25677..27698)
                     /gene="uvrB"
                     /locus_tag="OO130_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HCR5364317.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(28277..28954)
                     /gene="bioD"
                     /locus_tag="OO130_000809"
     CDS             complement(28277..28954)
                     /gene="bioD"
                     /locus_tag="OO130_000809"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCR5364318.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            complement(28947..29702)
                     /gene="bioC"
                     /locus_tag="OO130_000810"
     CDS             complement(28947..29702)
                     /gene="bioC"
                     /locus_tag="OO130_000810"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HCR5364319.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHAELQRRSADVLLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLVQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDEIEQSLNGVHYQHHIQPITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            complement(29689..30843)
                     /gene="bioF"
                     /locus_tag="OO130_000811"
     CDS             complement(29689..30843)
                     /gene="bioF"
                     /locus_tag="OO130_000811"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HCR5364320.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWQQGAEQFGVGSGGSGHVSGYSVAHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFVHNDVTHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYNTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            complement(30840..31880)
                     /gene="bioB"
                     /locus_tag="OO130_000812"
     CDS             complement(30840..31880)
                     /gene="bioB"
                     /locus_tag="OO130_000812"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HCR5364321.1"
                     /translation="MAHRPRWTLSQVAELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPNERDMPYLEQMVQGVKDLGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            31967..33256
                     /gene="bioA"
                     /locus_tag="OO130_000813"
     CDS             31967..33256
                     /gene="bioA"
                     /locus_tag="OO130_000813"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HCR5364322.1"
                     /translation="MTKDDLAFDQRHIWHPYTSMTSPLPVYPVVSAEGCELILSDGRR
                     LIDGMSSWWAAIHGYNHPQLNAAMKSQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEVRQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHNLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEYAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            33315..33791
                     /locus_tag="OO130_000814"
     CDS             33315..33791
                     /locus_tag="OO130_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HCR5364323.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVEHIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            33943..35226
                     /locus_tag="OO130_000815"
     CDS             33943..35226
                     /locus_tag="OO130_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HCR5364324.1"
                     /translation="MNTFSVSRLALALAFSVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     AFGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(35367..37604)
                     /locus_tag="OO130_000816"
     CDS             complement(35367..37604)
                     /locus_tag="OO130_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HCR5364325.1"
                     /translation="MFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSSHNTSGNMD
                     KLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGTINGDDHVF
                     GLSAAQRYGGIFVPPHIAIIHQYMREMMAGGGKMILGSDSHTRYGALGTMAVGEGGGE
                     LVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVKNKVMEFVG
                     PGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQLNPQPMAYY
                     DGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAHGKAKLSLL
                     DKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPSSQPVFMDL
                     AKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREGSKPANGQM
                     SAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQGFVKGATQQ
                     PLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRSNPIGLAEF
                     TLSRRDPGYVGRSKATAELENQRLAGNVSELTEVFARIKQIAGQEHIDPLQTEIGSMV
                     YAVKPGDGSAREQAASCQRVIGGLANIAEEYATKRYRSNVINWGMLPLQMAEVPTFEV
                     GDYIYIPGIKAALDNPGTTFKGYVIHEDAPVTEITLYMESLTAEEREIIKAGSLINFN
                     KNRQM"
BASE COUNT         8810 a        10183 c         9741 g         8876 t
ORIGIN      
        1 gcgtgggtgc gtttttggcg ggtgagaacg gtaactccac cactggcgac gccatttata
       61 tgcgtggtgc cgatacctct aacagtattt atattgatgg cattcgcgat atcggcagcg
      121 tctcgcgcga caccttcaat accgagcagg tcgaagtgat taaagggccg tccggcaccg
      181 actacgggca cagcgcaccg acgggctcga tcaatatgat cagcaaacag ccgcgcaatg
      241 attccggcat tgacgcctcc gccagtattg gcagcgcctg gttccgccgc ggcacgctgg
      301 acgtcaatca ggtcattggt gataccaccg cggtgcgcct gaatgtaatg ggcgaaaaaa
      361 cgcacgatgc cggacgcgac aaagtcaaaa atgagcgtta cggcgtcgcc ccttctgtcg
      421 cttttggcct tggtacagcg aatcgtttgt atcttaatta tctgcatgtc acccagcaca
      481 acacgccaga cggcggcatt ccgaccatcg gtttgccggg ctattctgcc ccatctgcgg
      541 gaacggcggc cctgaatcat tccggaaaag ttgatactca taacttttac ggcacggatt
      601 ccgattacga cgattcgacc accgacaccg ccaccatgcg ttttgagcac gacatcaacg
      661 ataacaccac cattcgcaat actacccgtt ggtcgcgcgt aaagcaggat tacctgatga
      721 cggcgattat gggcggggcg tcgaatatta ctcagcccac cagcgatgtg aatagctgga
      781 cctggtcacg cacggcgaat accaaagatg tgagtaataa aattctcacc aaccagacca
      841 acctgacctc gacgttctat accggttcta tcggtcatga tgtcagtacc ggcgtggaat
      901 ttacccgtga aacgcagact aactacggcg ttaatccggt gacgttaccc gcggtaaata
      961 tttatcatcc tgacagcagc attcatcccg gcggcctgac gcgcaacggc gcaaacgcca
     1021 atggtcagac ggataccttc gcaatttacg cctttgatac gctgcaaatc acccgtgatt
     1081 ttgagctgaa cggcgggatc cgtctggata attatcatac tgaatatgac agtgccaccg
     1141 cctgcggcgg cagcggacgc ggtgccatca cctgcccagc tggtgtggca aaaggttctc
     1201 cggtcaccac cgtcgacacc gccaagtcgg gcaatctggt gaactggaaa gccggggcgc
     1261 tgtatcacct gacggaaaac ggtaatgtct atattaacta tgccgtttcc cagcagcctc
     1321 cgggcggcaa caacttcgcc cttgcgcagt ctggcagcgg taacagtgcc aaccgcaccg
     1381 attttaaacc gcaaaaagcc aacaccagcg agattggcac caaatggcag gttctggata
     1441 aacgcctgtt gctcaccgcc gcactgttcc gtactgatat cgaaaatgaa gttgagcaaa
     1501 atgatgacgg aacttactcg caatacggta agaaacgtgt cgaaggctat gagatatccg
     1561 tggccgggaa tatcactccc gcgtggcagg tgattggcgg ctatacccag caaaaagcaa
     1621 ccatcaaaaa cggcaaagat gttgcccagg atggttcctc atcgctgccg tataccccgg
     1681 agcacgcctt caccttatgg agccagtatc aggcaaccga tgatatctct gttggcgcgg
     1741 gcgcacgcta tatcggcagt atgcataaag gttcagacgg cgcggtggga acgccagcgt
     1801 ttaccgaagg ttactgggtc gccgatgcca aactggggta tcgggttaat cgcaatctcg
     1861 acttccagct aaacgtttac aacctgtttg ataccgatta cgtcgcctca atcaacaaga
     1921 gcggctaccg ttatcacccg ggcaagccaa gaaccttctt gctcacagcc aatatgcatt
     1981 tctgattcag atgtggggcg caggccccac tttttggaga aattgtatga tgtaccacat
     2041 tcccggcgtg ttatcgccac aggacgtcgc tcgttttcgc gaacaactgg aacaagccga
     2101 atgggtggat ggacgcgtca ccaccggcgc acaaggtgcg caagttaaga acaatcaaca
     2161 ggtagacacc cgcagcacgt tatacgccgc cctgcaaaat gaggtgctga acgcggttaa
     2221 ccaacatgct ttattctttg ccgcggcctt gccgcgtacc ctttccacgc cgctgtttaa
     2281 tcgctatcag aacaatgaaa cctatggttt tcatgtggat ggcgcagtac gcagccatcc
     2341 acaaaacggc tggatgcgca ctgacctttc tgccacgctg tttttaagcg atccacaaag
     2401 ctacgacggc ggcgaactgg tcgttaatga caccttcgga caacatcggg taaaactccc
     2461 ggcaggtgat ctcgtgttgt atccctccag cagcctgcat tgcgtgacac ccgtaacccg
     2521 cggcgtacga gtggcatcat ttatgtggat ccagtcgatg atccgcgatg ataaaaagcg
     2581 cgccatgctg tttgaactag acaaaaatat tcagtcgctg aaaagccgct atggtgaaaa
     2641 tgaagagatc ctgtcgctgc tcaatcttta tcataatctg ctgcgggaat ggtcggagat
     2701 ctgacgcttg aactgcccag gttcgtccct atattagcta cacttaactg tacaagcatt
     2761 gatatgggga ggacgatatg gcatccggtt gggctaacga tgacgccgtc aacgaacaga
     2821 tcaacagtac aattgaagat gcgattgccc gcgctcgggg tgaaattccg cgtggcgaaa
     2881 gcctgtatga atgtgaagag tgcggtgccc ccatcccgca agcccgtcgg gaagccattc
     2941 ctggcgtgcg cttatgtatt cattgtcagc aggagaaaga tttacaaaaa ccagcgtata
     3001 caggatataa tcgcagaggt tcgaaagaca gccagttacg ttaactacaa ctggcgagat
     3061 gcatagcgag tataatttgt attttgcgtg gcattccgtg cctttaacgc tgctgtgact
     3121 gccacgcggc ataaaacaag tccataagta caacaaataa atggtttatc agtatgttag
     3181 atgtcaatca atttatttga acaaggcggt caattctctt cgattttatc tctcgaaaaa
     3241 aaacgtgata ctcatcacat cgacgaaaca acgtcactta tacaaaaatc acctgcgaga
     3301 gattaattat gaaaactatc aatactgttg ttgctgctat ggctctttca actctatcat
     3361 ttggcgtgtt cgcggcggaa ccggtaacgg catcccaggc acagaacatg aataaaatcg
     3421 gcgtggtttc tgccgatggc gcatccaccc tcgatgccct ggaagcgaaa ctgactgaga
     3481 aagccgcggc agccggtgcc agtggataca gcatcacttc tgccaccaac aacaataaat
     3541 taagcggtac tgcggtaatt tataagtaat tcgtaggacg gataaggcgt ttacgccgca
     3601 tccggcaaca gatgcctgat gcgacgctac cgcgtcttat caggcctaca aaatccgaac
     3661 cgttggacgg atcaggcggt tacgctacgt ccagcacaag tatttccctg tctgtttgcc
     3721 gacagacgca tatgctctaa ccctcattga tcctatatta cccttgtttg cccgtccgtt
     3781 actagacggg ctttttttag ctggctaact gctgacagaa agcctgcaac gtctcttccg
     3841 acataccaat ctgccgcgcc gcatcacaaa tggcctgcca gcttcccgca tccaatggaa
     3901 tcccctgctc ctgccgttcg cgacgcgtgt tcacttccca ctcgcccggt agcaaaatcg
     3961 gcttatcgtc atcatgcggc gaggctttca cccactcggc aaaggcttca gtctgcgcgc
     4021 tacaatccgg cgcgccgaac agttccgggt tgatgatgat agtggtcatg cagttaagaa
     4081 tggcatcggg actggtttgt aacgtttcct gatgcgtcgt tttaccgcca gaaagcgccc
     4141 cgccaagaat ttcacacatc gctgcaaggg cgtagccttt atgttcggca aaggtcaaca
     4201 gcgaccccaa cggcgactcc tgcattaccg ccggattggt cgtcggcacg ccgttaacgt
     4261 caatcaggca acctggcggc acggggacgc ctttatgcca ggcgacgcga gttttaccaa
     4321 atgcgatggc gctggtggcg taatcaagca acagcgggaa attatcttta cgagggaaaa
     4381 ccacacagaa cggattggtg ccaaagcggc tgtcgcgacc gtggaacggt gcgaccattg
     4441 gaataccgac cacgctaaca aagtggatag agacaaaccc cgccgctgca cactgctccg
     4501 cccagtaacc gatacggccg atatgatgcg agttatgtag cgccacggca gcaataccgt
     4561 gctgatgcgc tttctcaatc cccagcgcca tcgcttcgtg tgccgcgacc tgaccgaatg
     4621 cgcgatcgcc atcgagcgtg actgccgccc ccgcctcttt aacgattttg gcatgatggt
     4681 taatttgcag atacccctga ctccaggaac gcacatagct tgggatcatg ccaataccat
     4741 gagaatcatg ccctgccagg tttgccgcga ttaaatgatc ggccactaat ttcgcttctt
     4801 gttcctcgct acccatctga cgaaatacag cctgaataaa actgtgcagc gtctgagcat
     4861 caaagcgatg accactttcc atggttaact cctgtttatg ttatgtgttt gttgtttttt
     4921 tatgctgcac gccgggcgcg ggaaggtcaa taaagatgca ggaatttcat attgcggtgg
     4981 ggtaaaagcc ggatgacatg gctcatccgg ctgagaaaag aattaaaatg cctgattaac
     5041 gcgcgccagg ccgtcgctga tagttgccgc ttcacccgta gcctccaggc agcaagccat
     5101 ctggattttc agcgattcgg gaatcggttc gctgccagca aggcaacgct caatccactg
     5161 cgccgtggtt tctgggtctt ttgcttgtgg cagtaactcg ctaccagcag tgtcctgttt
     5221 ttcatacagc acccgcatcc cttcacggtc aatgagattg atctgcgggc agcgctgcgg
     5281 attagcatac acttcgcctt cagtaccatg cattagtagc gcccgcccgc cgatatcgct
     5341 aaagaacttc gcgactcgcc cgatgtattc cggatgcgaa acgctggaaa gacgcagcgc
     5401 ctcaccttcg gcaaatggcg tcgccagttt cgccagggta tgtgcactgt tacgcacgcc
     5461 catccgccag cgcatcgcca gttgtttttc cagcggcggg caaaacgcgc cgacaggcat
     5521 aaacaccggt tgatgttcgt cgagcttcgc ctgcgcctgc ccgccgtgca acgttggtgt
     5581 aatacccatc agttcgaaaa tggtttctgt cagcacgcga gttggatctt cgctaacccc
     5641 gtgaaccacc acaggaaaac cgagtttgtg caggagaata gccaacaacg gcgtcaggtt
     5701 ggcctgttta cgcgcgccgt tgtaactggg gatgacaatc ggcatcggct tgcctgcagg
     5761 cggcgtcagc ttgatggtgt gattttgcat ggcttcgtaa aagccgagca tctctgcttc
     5821 cccttcccct ttgatacgta gcgcaatcaa tacgccgccc aactcaaggt cagggacttc
     5881 accattgagc atatgggcat acagaccgcg cgcagtatcc cggtctaaat cgcgcgcgtg
     5941 gtttttcccg cgcccgatct ctttaatgat tttgcgatag tccatttacg actccttacc
     6001 tgactcacat cattaacgcc gccgacggcg tggggatttc gttttttctt tctttttaac
     6061 tataccttca gggacttccg gttgctctat cggaaatacc ggtaatgcat ccagtagtcg
     6121 cttgccataa tttttggtca gcaagcgttt gtcgtagata accacttcgc cccagcaacc
     6181 gtggcttcga atcagtcgcc caacctgctg aatcaggtta aacgaggcgc tcggcaggct
     6241 ttgcacctca aacggatagc ggttgaggct tttcagccat tccccttcgg tgatcaccac
     6301 cgggctgtcg atgggcggaa aagcgatttt gtggatatgc acctggctga gcagatcacc
     6361 tttcaaatca agcccttcgg caaatgactg taagcccacc agcacactac gctcaccgtt
     6421 ggcaacgcgt ttgcggtgca gttcaactaa gcgataacgc ggctgatcgc cctgcaccag
     6481 cagcatcagg cgtaaatccg tcacatagtc gagaaagcgc tgcatcgccc gtccgctggc
     6541 aaacagtacc aacataccga gatgtttttt gctctccacc tgctcacgga aaaaggccgc
     6601 catttcggca atatgctgct cttcgttgtc gatggaaggc tcaaagcgca tccggggaat
     6661 aacaattttg ccctgttcgc agtggttaaa gggggaatcc agcgccacaa aacggtcgcc
     6721 cgctttctct ttcagcccgc tcatctcctg caaacgcgaa aaactgttca gcgaacgcaa
     6781 ggttgcggag gtgacaataa tgtgcggaat actgcgccac agcagccttt ccagctgatc
     6841 gctgacacgt attcccacgc agtgaaacca gagatgcagc tgcccttcgc gctcttcccg
     6901 cgtcgcccat ttggtcaccg gcgcaccgga agattgcgcc agagacgcca gccgccagag
     6961 tttgctttgc gcctcgaaca tccccaacgc gcggttcatc tgcaaaatca accgatgcag
     7021 acgcacaata tcgtggctgc cggttttctc acttaaatcg ttaagaaata actccgccag
     7081 accacgcagc atctcggtga gttttgccag ccgctggcag atctccagca cttcatcagg
     7141 cagttcgccc atcgcaaaac ggtgctctgc ctcctgcccg gcaggcatgt agagattgag
     7201 aatgttgttt aacgaggcga taagctcata caactcttca caatgcgcat tcaaacgttc
     7261 agggatcgcc agcggtggga tggtcttcgg gcgaaactgc tccatgcagg tagcgaccag
     7321 tttcgtgaac aagtccagct gtagtcgata ccagggcgcg gtgatttcag cgctcatctc
     7381 cagcgcatcc cgggccacat ctggcaggtg atgaccttcg tccagcacca gcagcagatt
     7441 tttcgggtca ggcagtacgg cttcgctttc catcgccgcc atcaccagcg catggtttgc
     7501 caccaccact tccgcttcct gaatctcccg acgagcaaca aaaaacgggc attcacgata
     7561 gtagtaacag ttacggttaa ggcagctggc tttgtcggta ctcaaacgac gccagagatc
     7621 atcatctata gcgatatccg tatgatcgcg cagaccatcc catttataag tgtcgagatc
     7681 gcccttcagc ttcgcacaac gtttttgctc ttcctgatta tttggcgtca gttcgtcgtc
     7741 aagaaacgcc agcagatcct gttgcgtggg ttcagtgctg gcgagcgccg tcagattacg
     7801 cggacaaacg tagcgcccac gcccgaaagc ggcagtgaat ttaagatcgg gaatgatctt
     7861 tttcagcagc ggtaaatctt tgctgtaaat ctgatcctgc aacgccacgt tggcggtact
     7921 caccaccagc gttttttgct cttcgcgggc aatggcgatg ccggggatca aataggagag
     7981 cgttttccca acgccggtgg gggcttcaat cgccagatgc cgcccttctt ctccggccag
     8041 cgttttggcg acgtccgcaa tcatctgccg ctgcggcgca cggggaataa agtcggggat
     8101 ctgttcctga agcgccttat accaggcggc aatttgcgct ttaagcgcgg cggttaatgc
     8161 catgagaaaa cctgaaatac tgtataaaca gccaatattg tggcattttt cggttttcag
     8221 gggtaatggt ttttctgttt ctggaaagcg gctcgcaata accaccaaca taatgcggtt
     8281 taatgcccgt tcgagcacaa aggatccaac acgtcatgca agacaccatc ataaatttct
     8341 acagcaccag cgatgactac ggtgattttt ctaactttgc cgcctggccc atcaaggttg
     8401 acgggaaaac ctggcccacc tccgaacact attttcaggc acaaaaattc cttgatgaaa
     8461 aataccgcga agagatccgt cgggtttctt cccccatggt cgccgctcgc atggggcgta
     8521 atcgttccaa gccacttcgt aaaaactggg agtcggttaa agaacaggtg atgcgtaaag
     8581 cacttcgtgc taaattcgaa cagcatgcag aactgcgtgc gctcttgctg gcaaccgcgc
     8641 ccgcaaaact ggttgagcat acggaaaacg atgcttactg gggagacggt ggtaatggta
     8701 agggcaagaa tcgactgggc taccttttaa tggagttgcg cgaacaattg gctatagaga
     8761 agtaacgtct cggtggctta tgggcggtaa aacagataaa ttttttcccg tgttctcaaa
     8821 ttgctgacaa gtgcttgttg tgaaatgccg gatgcggcgt aaacgcctta tccggcctac
     8881 aaaagcatgc aaattcaata tattgcagag ttaacgtagg cctgataagc gtagcgcatc
     8941 aggcagtttt gcgtttgttc atcagcctga tgccgggcat agcccggcat aaaagattac
     9001 tgcgcagcgg caggtttacg cggacggcgg cgacgttgtt gttcgcctgc cggtttggca
     9061 tcgccgaggc ggcgagacgg tttttctgca ggtttcgcgc ttgcagattt tgcgccacct
     9121 tccccacggc gtggttgctg ttgaccgcga ccaccacctt gcccacgacc gccgccgcca
     9181 cgttgctggc gaccgttctg gatcggttcg gctttgattg acgggtccgg ctcatagccc
     9241 ggaatcgcaa tgcgcgggat ctcttttttc agcagttttt cgatatcacg cagcagtttg
     9301 tgttcatcaa cacacaccag cgacaacgct tcaccggtag cagccgcacg accggtacgc
     9361 ccgatacggt ggacataatc ttccggtacg tttggcagtt catagttgac gacgtgcggc
     9421 aactcttcga tatccagacc gcgcgcagcg atatcggtcg ccaccagtac gcgaatgtca
     9481 ctcgatttaa aatcagccag cgcacgggta cgcgcacctt gcgatttatt cccgtggatc
     9541 gccgcactac ggatgccatc tttattgagc tgctcagcca gatggttagc gccgtgtttg
     9601 gtacgggtaa acaccagcac ctgctgccag ttccctttac caatcatgtg cgacagcaat
     9661 tcgcgtttgc gtttcttatc gacaaagtga acgtgctggg tcacctgatc agacgcggta
     9721 ttgcggcgtg ccacttcgat ttccagcggg ttgtgcaaca gtttttccgc cagggcttta
     9781 atatcgtcag agaaggtcgc agagaacagc aggttctggc gtttcgctgg cagttttgtt
     9841 aacacgcgac ggatatcgtg gataaagccc atgtcgagca tgcggtcagc ttcatcgagg
     9901 acgaggattt caacctgatc cagcttcact gcattttgat gttccaggtc cagcaaacgt
     9961 cccggtgttg ccaccagaac atcaacgccg ccgcgcagct tcatcatttg cgggttaatg
    10021 ctgacaccgc caaacaccac cagcgaacga atgttcaaat atttgctgta atcacggacg
    10081 ttttcgccaa tctgcgcagc cagttcacgg gtcggggtaa gaatgagcgc acgtaccgga
    10141 cgacgccctt tggcgtgcgg ctggcgagtg atcaggtgtt gcaacagcgg cagcgtaaag
    10201 cccgctgttt taccggtacc ggtctgggcg ctagccatca ggtcgcggcc ttccagcacc
    10261 gcagggatcg cctgctgctg aataggggtg ggttcacggt aaccctgctc ggcaacggcg
    10321 cgcaggatat cagggcttaa acccaaagaa tcgaaagaca taactactcc gaaccgcccc
    10381 gaccgttacc ggtgtagttt tcagggagat actgaaaagg aaaatgacaa aaaccacaat
    10441 gtcatgaggg agcggagtgt agcagttttt gtgacgcagc gcataaatta tccctgtgac
    10501 tggcgttgct aaaattctta gccatactgg acaactcccc tatggagtca taatcttaat
    10561 caatcatttg attaagaatg gactgtgcga tgaataatcc tgccatgaca atcaagggtg
    10621 aacaggcgaa aaaacagctg attgctgccg cactggcgca gtttggtgaa tatggaatga
    10681 acaccaccac tcgcgagata gccgcccagg ccgggcagaa tatcgctgcc atcacctact
    10741 acttcggttc gaaagaagat ttgtacctcg cctgcgccca gtggattgcc gattttattg
    10801 gcgagcagtt ccgtccgcat gccgaggaag ccgaacgctt gttcgcgcaa ccacagcctg
    10861 atcgggctgc catccgtgaa ctgatccttc gcgcctgcag gaacatgatt aagctgctca
    10921 cccaggatga caccgtcaac ctcagcaagt ttatctcccg tgagcagctc tctcccacgg
    10981 cagcctacca cctggtgcac gaacaggtga ttagtccgct acacagccac ctgacacgcc
    11041 tgattgccgc ctggaccggc tgcgacgcca atgacacccg catgatcctt catacccatg
    11101 cgttgattgg tgagattctg gcgttccgtc ttggcaaaga aacaattctg ttacgtaccg
    11161 gctggaccgc gttcgatgaa gaaaagaccg aactgatcaa ccagacggtg acctgtcata
    11221 tcgacctgat tttgcaagga ttatcgcaaa ggagtttgta gtgatgaaaa aacctgtcgt
    11281 gatcggactg gcggtagtgg tacttgccgc cgtggttgcc ggaggctact ggtggtatca
    11341 aagccgccag gataacggcc tgaagctgta tggcaacgtg gatattcgta cggtgaatct
    11401 tagtttccgt gttggggggc gcgttgaatc gctggcggtg gacgaaggtg atgcgatcaa
    11461 agcgggccag gtgctgggcg aactggatca caagccgtat gagattgccc tgatgcaggt
    11521 gaaagcgggc gtctcggtgg cacaggcgca gtatgacctg atgcttgccg ggtatcgcga
    11581 tgaagaaatc gctcaggccg ccgcagcggt gaaacaggcg caagccgcct atgactatgc
    11641 gcagaacttc tataaccgcc agcaaggact gtggaaaagc cgcaccattt cggcaaatga
    11701 cctggaaaat gcccgctcct cgcgcgacca ggcacaggca acgctgaaat cagcacagga
    11761 taaattgcgt cagtaccgtt ccggtaaccg tgaacaggac atcgctcagg cgaaagccag
    11821 cctcgaacag gcgcaggcgc aactggcgca ggcggagctg aatttacagg actcaacgtt
    11881 gatagcaccg tctgatggca cactgttaac gcgcgcggtg gagccaggca cggttctcaa
    11941 tgaaggtggc acggtgttta ccgtttcact aacgcgtccg gtgtgggtgc gcgcttatgt
    12001 tgatgaacgg aatcttgacc aggcccagcc ggggcgcaaa gtgctgcttt ataccgatgg
    12061 tcgcccggac aagccgtatc acgggcagat tggtttcgtt tcgccgactg ctgaatttac
    12121 cccgaaaacc gtcgaaacac cggatctgcg taccgacctc gtctatcgcc tgcgtattgt
    12181 ggtgaccgac gccgatgatg cgttacgcca gggaatgccg gtgacggtgc aattcggtaa
    12241 cgaggcagga catgaatgat gccgttatca cgctgaacgg cctggaaaaa cgctttccgg
    12301 gcatgggcaa gcccgccgtc gcgccgctcg attgtaccat tcacgccggt tatgtgacgg
    12361 ggctggtggg gccggatggt gcaggtaaaa ccacgctaat gcggatgctg gcgggattac
    12421 tgaaacccga cagcggcaat gccacgatga ttggctttga tccaatcaaa aacgacggcg
    12481 cgctgcacgc cgtgctcggc tatatgccgc agaaatttgg tctgtatgaa gatctcacgg
    12541 tgatggagaa cctcaatctg tacgcggatt tgcgcagcgt caccggcgag gcacgtaagc
    12601 aaacttttgc tcgcctgctg gagtttacgt ctcttgggcc gtttaccgga cgcctggcgg
    12661 gcaagctctc cggtgggatg aaacaaaaac tcggtctggc ctgtaccctg gtgggcgaac
    12721 cgaaagtgtt gctgctcgat gaacccggcg tcggcgttga ccctatctca cggcgcgaac
    12781 tgtggcagat ggtgcatgag ctggcaagcg aagggatgtt aatcctctgg agtacctcgt
    12841 atctcgacga agccgagcag tgccgtgacg tgttactgat gaatgaaggc gagttgctgt
    12901 atcaggggga accaaaagcc ctgacacaaa ccatggccgg acgcagcttt ctgatgacca
    12961 gtccacacga gggcaaccgc aaactgttgc aacgcgcctt gaaactgcca caggtcagcg
    13021 acggcatgat tcaggggaaa tcggtacgtc tgatcctcaa aaaagaggcc acaccagacg
    13081 atattcgcca tgccgacggg atgccggaaa tcaacatcaa cgaaactacg ccgcgttttg
    13141 aagatgcatt tattgatttg ctgggtggtg ccggaacctc agaatcgccg ctgggtgcaa
    13201 tattgcatac ggtagaaggc acacccggcg agacggtgat cgaagcgaaa gaactgacca
    13261 agaaatttgg tgattttgcc gccaccgatc acgtcaactt tgccgttaaa cgtggggaga
    13321 tttttggttt gctggggcca aacggcgcgg gtaaatcgac cacctttaag atgatgtgcg
    13381 gtttgctggt gccgacttcc ggccaggcgc tggtgctggg gatggatctg aaagagagtt
    13441 ccggtaaagc gcgccagcat ctcggctata tggcgcaaaa attttcgctc tacggcaacc
    13501 tgacggtcga acagaattta cgctttttct ctggtgtgta tggcttacgc ggtcgggcgc
    13561 agaacgaaaa aatctcccgt atgagcgagg catttggcct gaaaagtatc gcctcccacg
    13621 cgaccgatga actgccatta ggttttaaac agcggctggc gctggcctgt tcgctgatgc
    13681 atgaaccgga cattctgttt ctcgacgaac caacgtccgg cgttgatccc ctcacccgcc
    13741 gcgaattttg gctgcatatc aacagcatgg tagagaaagg cgtcacggtg atggtcacca
    13801 ctcactttat ggatgaagca gaatattgcg accgcatcgg cctggtgtac cgtgggaaat
    13861 taattgccag cggcacgccg gatgatttga aagcgcagtc ggccaacgac gagcaacccg
    13921 atcccaccat ggagcaagcc tttattcagt tgatccacga ctgggataag gagcatagca
    13981 atgagtaacc cgatcctgtc ctggcgtcgc gtacgggcgc tgtgcgttaa agagacgcgg
    14041 cagatcgttc gcgatccgag tagctggctg attgcggtag tgatcccgct gctactgctg
    14101 tttatttttg gttacggcat taacctcgac tccagcaagc tgcgggtcgg gattttactg
    14161 gaacagcgta gcgaagcggc gctggatttc acccacacca tgaccggttc gccctacatc
    14221 gacgccacca tcagcgataa ccgtcaggaa ctgatcgcca aaatgcaggc ggggaaaatt
    14281 cgcggtctgg tggttattcc ggtggatttt gcggaacaga tggagcgcgc caacgccacc
    14341 gcaccgattc aggtgatcac cgacggcagt gagccgaata ccgctaactt tgtacagggg
    14401 tatgtcgaag ggatctggca gatctggcaa atgcagcgag cggaggacaa cgggcagact
    14461 tttgaaccgc ttattgatgt acaaacccgc tactggttta acccggcggc gattagccag
    14521 cacttcatta tccccggtgc ggtgaccatt atcatgacgg tcatcggcgc gattctcacc
    14581 tcgctggtgg tggcgcgaga atgggaacgc ggcaccatgg aggctctgct ctctacggag
    14641 attacccgca cggaactgct gctgtgtaag ctgatccctt attactttct cgggatgctg
    14701 gcgatgttgc tgtgtatgct ggtgtcagtg tttattctcg gcgtgccgta tcgcgggtcg
    14761 ctgctgattc tgttttttat ctccagcctg tttttactca gtaccctggg gatggggctg
    14821 ctgatttcca cgattacccg caaccagttc aatgccgctc aggtcgccct gaacgccgct
    14881 tttctgccgt cgattatgct ttccggcttt atttttcaga tcgacagtat gcccgcggtg
    14941 atccgcgcgg tgacgtacat tattcccgct cgttatttcg tcagcaccct gcaaagcctg
    15001 ttcctcgccg ggaatattcc agtggtgctg gtggtaaacg tgctgttttt gatcgcttcg
    15061 gcggtgatgt ttatcggcct gacgtggctg aaaaccaaac gtcggctgga ttagggagaa
    15121 gagcatgttt catcgcttat ggacgttaat ccgcaaagag ttgcagtcgc tgctgcgcga
    15181 accgcaaacc cgcgcgattc tgattttacc tgtgcttatt caggtgattc tgttcccgtt
    15241 cgccgccacg ctggaagtga ctaacgccac catcgccatc tacgatgaag ataacggcga
    15301 gcattcggtg gagctgaccc aacgttttgc ccgcgccagc gcctttactc atgtgctgct
    15361 gctgaaaagc ccacaggaga tccgcccaac catcgacaca caaaaggcgt tactcctggt
    15421 gcgtttcccg gctgacttct cgcgcaaact ggataccttc cagaccgcgc cgttgcagtt
    15481 gatcctcgac gggcgtaact ccaacagtgc gcaaattgcc accaactacc tgcaacagat
    15541 cgtcaaaaat tatcagcagg aactgctgga aggaaaaccg aaacctaaca acagcgagct
    15601 ggtggtacgc aactggtata acccgaatct cgactacaaa tggtttgtgg tgccgtcact
    15661 gatcgccatg atcaccacta tcggcgtaat gatcgtcact tcactttccg tcgcccgcga
    15721 acgtgaacaa ggtacgctcg atcagctact ggtttcgccg ctcaccacct ggcagatctt
    15781 catcggcaaa gccgtaccgg cgttaattgt cgccaccttc caggccacca ttgtgctggc
    15841 gattggtatc tgggcgtatc aaatcccctt cgccggatcg ctggcgctgt tctactttac
    15901 gatggtgatt tacggtttat cgctggtggg attcggtctg ttgatttcat cactctgctc
    15961 gacacaacag caggcgttta tcggcgtgtt tgtctttatg atgcccgcca ttctcctttc
    16021 cggttacgtt tcgccggtgg aaaacatgcc agtatggctg caaaacctga cgtggattaa
    16081 ccctattcgc cactttacgg acattaccaa gcagatttat ttgaaggatg cgagtctgga
    16141 tattgtgtgg aatagtttgt ggccgctact ggtgataacg gccacgacag ggtcagcggc
    16201 gtacgcgatg tttagacgta aggtgatgta acttcttatc tttcgccagc aaagacacta
    16261 ccgccgggcc ggcaaggatt gccagccctg caattgccag caaaaagttg ttttccagta
    16321 cgcgggacaa cagccacagc acaatcagcg ctgcgccaaa ataccaggtc gtggtcagct
    16381 cttccaacac gtctgccacc tcacgccagc gttgctcggg gtggcgctga aacaccaaca
    16441 tcatcactac cgtgacacaa tacagtgcgc ataatcccac gccgacgtga accagagtct
    16501 tactcatcca gcccaccacc agcagcgcca ctaccagtaa atgcaacata atctgccagc
    16561 aacttagacc cgttgcgaca cgaacacgtt gttgccactt catggcttct ctcctgaaga
    16621 atttatctct acttactcag agtaccgaac tttacggaaa attccgtaag accgcacctt
    16681 tttttgacgc cgactacact atttcaatga gataagaggc aaagacagga aaattatgcc
    16741 cgatcaaaca caacaatttt cgttcaaggt gctcaccatc aacattcaaa aaggttttac
    16801 cgcgtttaac cgacgcttca ttttgccgga acttcgcgac gcggtgcgta ccgtcagcgc
    16861 cgatattgtt tgcctgcagg aagtgatggg cgcgcacgaa gttcatccgc tgcatgtgga
    16921 aaactggccc gatacctcgc actacgagtt tctcgccgac actatgtgga gcgattttgc
    16981 ctacggtcgc aatgccgtat acccggaagg gcatcacggc aacgccgtac tgtcgcgtta
    17041 tcccattgaa cattatgaga atcgcgatgt ttcggtcgat ggtgcggaaa agcgcggcgt
    17101 gctctactgc cgcattgtgc cgccgatgac cggaaaagcg attcatgtga tgtgcgtaca
    17161 tctgggcctg cgtgaggcgc accgtcaggc gcagcttgcg atggggtgaa tgagctaccg
    17221 gacggcgaac cggtattggt ggcgggtgat ttcaatgact ggcggcaaaa agctaatcat
    17281 ccgttaaaag tgcaggccgg actggatgag atttttaccc gcgcccacgg acgcccggcg
    17341 cgcacgtttc cggtgcaatt tcctctacta cgactggaca ggatctacgt caaaaatgcc
    17401 agcgccagcg cgccaaccgc gttgccgctg cggacatggc gacacctttc tgatcatgcc
    17461 cctttaagtg cggagattca tttatgaaat gtagctggcg cgaaggcaat aagatccagt
    17521 tgctggaaaa cggcgagcaa tattatcccg cggtgtttaa ggcgattggc gaggcacaag
    17581 aacgcatcat tcttgaaacg tttatctggt ttgaggataa cgtcggcaaa cagctgcatg
    17641 cggcactact ggcagcagcg caacgcgggg ttaaagcgga agtcttgctg gatggctacg
    17701 gttcgccgga tctcagcgat gagtttgtca ataaactgac ggcagctggc gtagtgttcc
    17761 gctactacga tccccgccct cgcctttttg gtatgcgcac caatgtgttt cgccggatgc
    17821 atcgcaaaat tgtggtgatc gacgcgcgta tcgcctttat tggcgggctg aattactccg
    17881 ccgagcatat gtccagctac ggtccagagg ctaaacagga ttacgcggta cgccttgaag
    17941 ggccgattgt cgaagatatt ctccagtttg agctggaaaa cctgcctgga cagagcgcgg
    18001 cacggcgctg gtggcgacgt catcacaaag cggaagagaa ccgccagccg ggagaagcgc
    18061 aggtattgct ggtctggcgc gataacgaag aacatcgcga tgatattgaa cgccattatc
    18121 tgaaaatgct cactcaggcg cggcgggaag tgattatcgc caacgcctac ttcttccccg
    18181 gctatcgatt tttacacgcc ttgcgtaaag cggcacggcg cggggtgcgg atcaaactga
    18241 tcattcaggg cgaaccggat atgccgattg tcagagtcgg tgcgcgcttg ctgtataact
    18301 atctggttaa aggcggcgtt caggtttttg agtaccgccg ccgcccgctc cacggcaaag
    18361 tggcattgat ggacgatcac tgggcgacag tagggtccag taatctcgat ccgctcagtt
    18421 tgtcactgaa tctcgaagca aatgtcatca tccacgatcg tcattttaac cagacgctgc
    18481 gcgataatct gaacggcatt attgccgcag attgtcagca ggtggatgaa accatgctgc
    18541 ccaaacgcac ctggtggaac ctgaccaaaa gcgtgctggc gttccacttt ttacgccact
    18601 tcccggcgct ggttggctgg catccggcac acacaccacg tctgacgcag gttgatcccc
    18661 ccgcacaacc gacaatggaa acgcaggatc gggtagaaac tgaaaacacg ggggtaaaac
    18721 cctgatgagt aaatcacacc cgcgctagcg cttagcaaag aagatcctca cctggctgtt
    18781 ttttatcgcg gtgattgtgt tgctggtggt ctacgccaaa aaagtggact gggaagaggt
    18841 ctggaaggtc atccgcgact acaatcgcgt tgcgctgctt agtgcggtcg ggctggtggt
    18901 cgtcagctat ctgatttacg gctgctatga cctgctcgcc cgcttttact gcggtcacaa
    18961 actggcgaag cgccaggtga tgctggtgtc gtttatctgc tacgccttca acctgacgct
    19021 cagtacctgg gtcggcggca ttggtatgcg ctatcgtttg tactcccgac tagggttacc
    19081 gggcagcact attacgcgga ttttctcgct cagtattacc accaactggc tgggctatat
    19141 tttactggca gggattatct ttaccgcagg cgtggtggtg ttgccggatc actggtatgt
    19201 cgctcaaact acgctgcgta ttctcggcat tggcttactg atgattatcg cggtttattt
    19261 gtggttttgc gctttcgcga agcaccgcca tatgaccatc aaagggcaaa agctggtgct
    19321 gccttcatgg aaattcgccc tcactcagat gctgatttcc agcgttaact ggatggtaat
    19381 gggggcgatt atctggctgt tacttggtca aagcgtgaac tatttctttg tactgggcgt
    19441 gttactggtt agtagtattg ctggcgtcat cgtgcatatt ccggcgggga tcggtgtgct
    19501 ggaagcggtg tttatcgcac tactggctgg ggagcatacc tcgaaaggga gcattatcgc
    19561 cgcgctgctc gcttatcgcg tcctgtatta ctttatcccg ctgctgctgg cgctgatctg
    19621 ttacctgcta ctggaaagcc aggcgaagaa gctacgggcg aaaaatgaag cggcgatgtg
    19681 aaatttagtt aagttcttta gtatctgcat tcacgactaa tgaaaaaaac gcgtatgcct
    19741 ttgccagaca agcgttatag ctatcgcggc aagtatccca aagaagccga taaaatagta
    19801 atagagtgtc aatgcacctg taaaagcgca acgaaccgga ctccacaact cttcactatg
    19861 gcattttcct gccagttcaa ggagcttatt tcgtccatgc gagataatgc ctggccataa
    19921 cacaatcact aagcaactta ttacccaaat gggccgttca ctcattaatg ccgcattcac
    19981 gatgattacc agggccaacc ctgtcaacgt catcataatg ataaaacggt gagaaccagg
    20041 atcaacatta aataataaac cggccagcat actgattaaa aacatcgcgc ccgcagtgct
    20101 gaacacaatg ataatggcat atacaggaac taaacttgca acgacaatcg ccaccaaaga
    20161 gagtaaaaac agatatatta cgcctgcgat gacggcattt atcttgttga tatccttact
    20221 taagccatac atacaaacta ataataaccc tatggcgaca tacattgttg gtgaatcata
    20281 tttggttgcc gtaatttcga gatacttagc tccccaggcc agtcctgcgg ttgccaccaa
    20341 acctaaagcg gtaatgagcc atataccgtt taatatcctg gtctcgtgct gaaaatctaa
    20401 tttattgctg ttttgtgagt atgattccat tcttttagtt gtccttagaa aaaatattaa
    20461 tattcaccat caataaattt aaacccacgc atacaaaata cttttgtatt tatggatacg
    20521 gaaaacgctt ccgctcggcc ataaggcgta ttttatacct ggtacttgtt aagtgtcgtt
    20581 tttagtaaga aaagaaatcc cgacacatgg ccgggatatt gatggtgaaa aagaattaac
    20641 ggcggttgcc aaaaattcgc agcaacatca ggaacaggtt gatgaagtcc agatacaagg
    20701 ttaacgcgcc aagaatggaa tatttgcgca ggttcgacgt gtcgcgggta tcaatctgct
    20761 cacccatatt tttcaatttc tgagtgtcat acgccgtcaa tccgacaaag acaatcacgc
    20821 cgatgtaggt aactgcccac atcaatgctt cgcttttcag ccagaagttg accagcgatg
    20881 ccagcacaat gccgattaac gccataaaca gcatattgcc aaagccactt aaatcgcgct
    20941 tcgtggtgta accgtacagg ctcattgcgc cgaacatccc ggcagtaacg acgaaagtac
    21001 tggcgataga agcagcggta tagacaatga atatactgga aagcgtaaga cccgtcagcg
    21061 ccgaataaag cataaagagc atcgtcgtta cacctgcgct cagcttttga atcatcgctg
    21121 ataacacaat aaccaatgct aattgcgcga tgatcagacc gattaaaaag acacggttag
    21181 tgaacaacag ctccatcacg gccgcggaat tagccgcata ccaggcaaca aatgcggtca
    21241 gcaacaagcc aacggtcatc cagccataga cttgcgccat ataagtttgc aagccagccc
    21301 ggggttgtac gattgaatca gaacgtggga atctgtccat gacgatctcc tgaagatata
    21361 aggaatatct taaggatact gcaaaatgat gaggctgtgc atcgacgcag cgtaaacgca
    21421 tgtactgagc ggtgaaattg ccggacgcag cggtgcctta tccggctaac aaaaaactac
    21481 cagcgttttg ccgcctgctg atcgctctcc cgagcttcaa cccagcggtc gccttccggc
    21541 gtggcttcgc gcttccagaa cggtgcgcgg gttttgagat aatccataat aaactgcccg
    21601 gcttcaaacg cactgctgcg atgcgcactg gtgacaccgg caaaaacgat ttcatcgccc
    21661 ggccataatt ccccgatgcg gtgaatcaca gtgacgcgcc ccagcggcca gcggttacgc
    21721 gcttcatcaa caatttctgc cagtgctttt tcagtcattc ccggatagtg ttcgagggtt
    21781 aatgctttga cgctgtcgcc caggttatgg ttgcgcacct taccagtaaa ggtgactacc
    21841 gcaccgtctt cgtcacgctc cgccagccac gggtactctt ctcctacgct gaacggctgc
    21901 ggaccaacaa caattttggt ttctgccatc ttaacctccg gttaccggcg ggaagaaagc
    21961 tacttcgtcg ccgtcagtca gcggatggtc aaaactcacc agcgtctggt tgacggcagc
    22021 cagtaatttg ccatcttcca gcgccagcgc ccagcgatcg ctctgcgcag ccatgtgctg
    22081 gcgtaacgct tcaacagttg ggaaatccgc agccacttcg gttgcatctg ttcccaccaa
    22141 ctcgcgcacc tgcgcgaaaa aaagaacttt aatcatccgc ttccacctta aagtcacccg
    22201 acttgccgcc gctcttcgcc agcaaacgta ccggaccaat caccatatct ttttgcaccg
    22261 ctttgcacat gtcataaatg gtcagcgccg ccacggaggc cgcggttaat gcttccattt
    22321 cgacaccggt tttcccggtc aggcggcata aggtttctat acgcacccga ttgtgctccg
    22381 gctcggcctg taaattgact tcaactttgc tgagcatcag cggatgacag agcgggatca
    22441 gatcccaggt gcgttttgcc gcctgaatac cggcaatacg cgcagtggca aatacgtcgc
    22501 ctttgtggtg gcgaccatca ataatcatcg ccagcgtctc gctgcgcatg gtgacaaagg
    22561 cttccgcccg cgcttcacgc acggtttccg ctttggcgga gacatccacc atgtgcgctt
    22621 cgccagcggc gttgatatgg gtcagttgcg acatacttat ttcttcaaat gtggatggaa
    22681 attacacgga cgcgtacggg catccagctg cggcgcgatg atattttccc atgcggtacg
    22741 gcacgctttg gtcgaacccg gcatggcgaa aatcagcgtt ttgttggcga cgcccgcaac
    22801 cgcacgagat tgcaacgtgg aagtgccaat ctcttcaaac gacaacatcc ggaacacttc
    22861 accaaaacct tcaacttcac ggtcgaacaa cggcagcaat gcttcgggag cctgatcacc
    22921 ttccgtcagg ccagtaccac ccgtaatcaa taccacttgt acatcgtcgc tggcgatcca
    22981 cgcagatacc tgagcgcgaa tagcgtagcg gttttctttc acaatggctt tatcgacaac
    23041 gtgatggccc gcttcttgcg ccgaatcgcg cagatagtga ccggaggtat cgtcttcttc
    23101 accgcgacga ttagaaaccg taagaatagc aatacgggtc gggataaatt cagtgcttac
    23161 ctgactcatc tgatctctcc ttttgacgtt ttagccgcca atgtacgata agttttgcgt
    23221 aataccggtg ttgttttgat gcaggaaatg ggtctgtttc ttctcccgca gcgccgctga
    23281 aatacgcgct tccagcgcct gttgctgggt atcgtcttcc agcagatcgc gcaggttaac
    23341 gccgccttca ccaaacaggc agagatggag tttaccaatg gaggaaacgc gcaggcggtt
    23401 gcaagtggcg cagaagtctt tttcatacgg catgataagg ccaatctctc cggcgtaatc
    23461 tggatggcaa aagacttgcg cgggaccgtc gctgcgttga cgtaattggt ggatccagcc
    23521 gcgacgcagt agctcgtcac gcagaacctg accagagatg tgatgcttac ggaagagctc
    23581 gctgccctcg cccgtttcca tcagttcgat gaaacgcagc tggatagggc gatgctggat
    23641 ccagttcaga aaggtgtcga gctggtgatg attaacatca cgcatcagca cggtattgac
    23701 tttgaccttc tcaaaaccgg cctcaaatgc agcatcaatc cctgccatga cctggttgaa
    23761 tttatcctgc ccggtaatag cgtgaaactg gcgggcgtcc agactgtcga cactgacgtt
    23821 aatgccagta agtcccgcat cgcgccagtt cgccacatcg cgttccagac ggtaaccatt
    23881 ggtggtgacc gcaatctggc ggatagcgtc gttttcccgc acagcggcga tgatatcggt
    23941 aaagtcgcgg cgtaaagacg gctcacctcc tgtcaggcgc actttttcgg tgcccagact
    24001 ggcgaaggcg cgcgtaaccc ggcgaatttc atcgacggta agaaagcctt tattggtgac
    24061 gccgctcggt ttgtagccat ccggcaggca gtaggtgcaa cgaaagttac acacatcggt
    24121 aatcgacagg cgcaagtagt aaaacttacg cgcaaatgca tcagtcagtt gtgaagccat
    24181 gtacaccttt ccagatacgg gaggcgaagt catttcttcc ttcgccctgg tggcaatctt
    24241 tacgagcatt gtcacggcca aagcaccgta tcagttgacc caggtgcaga ggctagagtg
    24301 tttagtggtt aagccgatac tagcgtgtaa atgagatttt taccatccac actttcgcta
    24361 tgcaatcatg tatatagcgt catgatcgtg ctatttcgct acagaataag ggaaaatggc
    24421 agttttactt tgatatacat catcagggag gcatagcgtg catcgtcctt tagaggtaga
    24481 agaaaatagt ttgtatcctc aatattggca ggttaattgc tgtttccccg caatttgcgc
    24541 tactgtagcg cggataatct gactccagga aactatatgc gcaatcgtac gctggctgat
    24601 cttgatcgtg tcgttgctct cggcggaggg catggactgg gacgcgttct ctcatcactt
    24661 tcgtctttgg gttctcgttt aacgggtatc gtcaccacca cggataatgg tggctcgacg
    24721 gggcgtattc gccgttcaga aggcggcatt gcctggggcg atatgcgcaa ctgcctcaac
    24781 caactgataa cggaaccgag cgtcgcctcc gcgatgtttg aataccgttt tggcggcaat
    24841 ggcgaacttt ccgggcacaa ccttggaaac ttgatgttaa aggcgctgga tcaccttagc
    24901 gtgcggcctc tggaagccat caatttaatt cgtaatctgc tgaaagtgga tacgcatttg
    24961 attccaatgt cagagcatcc cgtcgatctg atggcgattg acgatcaggg gcatgaagtt
    25021 tacggcgagg tcaatatcga ccagttaact acgccgattc aagagttatt attaacgccg
    25081 aatgtacccg caacgcgtga ggcagttcac gctatcaatg aagcggatct catcattatt
    25141 gggcctggca gtttttatac cagcctgatg ccaattctgc tgctgaagga aatcgcccag
    25201 gcattacgcc gcacgccagc gccgatggtt tatatcggca atctggggcg tgagttgagt
    25261 ttacctgcgg ctaatttgaa gctggaaagc aagctggcaa ttatggagca gtatgttggt
    25321 aaaaaagtca ttgatgcggt catcgtcggg ccaaaagtgg atgtctcggc ggtgaaagag
    25381 cggattgtga tccaggaggt actggaggcc agcgatattc cgtatcgtca tgaccgccag
    25441 ttgttacata acgcgctgga aaaggcgtta caggctttag gttaacggaa atccgaagga
    25501 aaattccggc ttcctattga agacaaagtg cgcgttgttt atgccggatg cggcgtgaac
    25561 gccttatccg gcctacaaac cgcgcaaatt caatatattg cggagaaaat gtaggcctga
    25621 taagcgtagc gcatcaggct gttttccgtt tgtcatcagt cttcttcgct atcctgttac
    25681 gatgcggcaa taaacaactc acgcaactga tgcaactggt cacgaatttg tgccgcttct
    25741 tcgaactcca gattctgcgc gtgttgcatc atcagccctt ccagttcatg gattttctgc
    25801 tgcaacgctt tcggcgacat atccatcggc acattatccg gctcaacaat cgggcgcgat
    25861 tttcctctgc ccttcgcttt ggttttggca atgttctgcc ccagcgccag gatatcgacc
    25921 actttcttgt tcaagccttg cggcgtaatg ccgtgttcct cgttgtactt ctgctgtttc
    25981 tcacggcgac gttcggtttc gccaatcgct ttcgccattg atggggtgat cttatcgccg
    26041 tagagaatcg ctttaccgtt aacgttacgt gccgcacgac caatggtctg gatcaacgaa
    26101 cgttcggaac gcaggaagcc ttctttgtca gcgtcgagga tcgccaccag cgacacttcc
    26161 ggcatatcca gaccttcgcg cagtaagttg atccctacca gcacgtcgaa ctcacccaga
    26221 cgcaagtcgc ggataatctc catacgttcg acggtgtcga tatctgagtg aagataacgc
    26281 acgcgctcgc catgttcttc gagatattcg gtaagatctt ccgccatccg cttggtcagt
    26341 gtggtgacca gtacgcgttc gttaattgcc gctcgctgac gaatctccga aagaagatca
    26401 tcaacctgtg tcgccaccgg ccgcacttcg ataatcgggt caagcaatcc ggttggacgc
    26461 accacctgat ccaccacatc gccgccggat ttttccagct cgtaattacc cggcgtcgcc
    26521 gaaacataga tggtttgcgg cgctaatgct tcgaactctt caaacttaag cggacggtta
    26581 tccagcgctg atggcaggcg gaagccgtac tccaccagtg tctctttacg cgcccggtca
    26641 ccgcgataca tgccgccaat ttgtggaatg gtgacgtgag attcatcgac gaccagcagc
    26701 ccatcggcag gcaggtaatc aaacagcgtc ggcggtggct cacccggtcc acgaccggag
    26761 aggaagcgcg agtagttttc aatccccgaa cagtagccca gctcgttcat catctccaga
    26821 tcaaactggg tacgctgggt cagccgctgc tcttccagca gtttgttgtt ttccaacagc
    26881 actttgcgtc tggcggccag ttcttctttg atctcctcca tcgcctgtac gatgcgctcg
    26941 cgcggtgtga cgtagtgcgt tttcgggtag atggtaaaac gtggaatagt ggaaacaatc
    27001 tgcccggtca gcgggtcaaa taacgacaat cgttccactt cctcgtcaaa cagttccacg
    27061 cgaagtgcaa tgtcatccga ttctgccggg aagatatcaa tcacctcgcc acgaacgcgg
    27121 aaagtaccac gctggaatgc ttgatcatta cgagcgtatt gcagctccgc cagtcggcgc
    27181 agaatcgcgc gctgatcgat aatcataccg accgtgagat ggagcatcat cttgagatat
    27241 aaatcaggat cgcccagacc ataaatcgcg gaaacagagg ccactacaac cacatcacgc
    27301 cgctccagca tcgctttggt ggcggataaa cgcatctgct caatatgttc gttaaccgag
    27361 gcatctttct caatgaaagt gtcggaactc ggtacatagg cttccggctg atagtagtcg
    27421 tagtaggaga cgaaatactc caccgcgttt tccgggaaga actctttcat ttcgccatac
    27481 agctgggccg ccagcgtttt gttgggcgca agtaccatgg ttgggcgctg aaggtcagca
    27541 atgacattgg caatggtgaa ggttttccct gagccagtca cgccaagtaa cgtctggtgc
    27601 gccaggccat cttccagccc ctcttcgaga cgtcgaatcg cctctggctg atcgccagaa
    27661 ggtttaaaag cggaattcag tttgaacggt ttactcatga gtcgctacct gaaggagttg
    27721 ggcgggcagg tatgtaattt tactcgtcgt acttaattat gccaacaaat tatactggat
    27781 aaaaaaacag ttcatcacca taatatttct gatacagcgt aaactccgct gtcaatcatg
    27841 agcaaaattt actctgtggc gagataaaac tccggcctta ccgggttatc cccaaagcaa
    27901 cggctttttt aacatttgtc aagatgagtg atgacagttt tatggcaaga gatgcctgtt
    27961 cagtgactgc cattgcattt atctaaccag ttaaaaaata aaagatattt ctttgagccg
    28021 tctttaacgc caattcgcgt gcaagccgcg tattctctcg cttgcctcgt gttttctaac
    28081 tctaatacac atggttatcc acaggaatag tggataactg tctccagccc ctatcccccg
    28141 ccgcctgggc aattcccaca ttcgccgcat gaggcggaag taaaattttt ttcgtgatta
    28201 ttttagaatt taattggtta aattgcagtc aatcgaagac gcgatctcgc tcgcaattta
    28261 accaaataca gaatggctac aacaaggcaa ggtttatgta ctttccggtt gccgcatttt
    28321 ctggattttc tgcaagccag gggatctctc ccagcagcgg cgcgggaatc atgcgggtga
    28381 gcgtggtcat atattcagcg tgacgttttc ccggaggcgt aacatcgttc gccacccaac
    28441 ccgccagagt cagtccggcg tgttgtattg cctgcgcagt caacatcgcg tgattaatac
    28501 agccgagttt cacaccaact accagtatca ccggcagttg ttcctgtgtt acccaatctg
    28561 caaaagtgaa agtgtcagaa agcggcgtaa accagccgcc agcaccttcc actaacaccc
    28621 agtcagcctg ttgttcaagc gcgcgtaatc cggcgctcat taccaatgat tctatcggcc
    28681 tgccctcttg cgcgctgatg atgtgcggcg aagtgggttc tgcgaaggtg taaggattta
    28741 ctgttgcgta atccagctgc aggctgctgt tgtgctgtaa cgccagcgcg tcactattac
    28801 gtaagccttc cggggtcttt tcgctgccag aggcgaccgg tttataacct gccgtccgat
    28861 aacctactgc cttcgcggct tgtaaaagtg cacaactggc gacggttttc cccacttcgg
    28921 tatccgttcc ggtgacaaaa taacgtttac tcacgagcaa tcactcccaa aaaaagatga
    28981 tacgtcagag gatatcgccc ctgttgttgc ggccaggcca gttgcaatcg ctgcaattgc
    29041 gaacgcgtta atattcgcgg gtcgcgccct tcatgaagat gcgtagcacc gatgcctttc
    29101 agcgaacgca tggcactgag cgcatcatca aaccatagtg tgatgggctg aatatgatgt
    29161 tgataatgca cgccgttcag cgactgttcg atttcatctg gcggtaaaaa gcgattagca
    29221 tgcggacgct cgtccaccgc ctgccacgcc tgatgcagtt cgggtaacga tccctgcacc
    29281 agcgtggtaa acgccaccac gcctccgggg cgcaccaccc gatacagctc gcggagtgcc
    29341 gtggataaat taccgcacca ctgcaccgcg agattgctcc atgcaagatc gaacgtcgca
    29401 gtcgctaacg gcagggattc gatatctccc gccagataat ggtctgcagc atctttctgg
    29461 cgggcttgca caagcattgg cggtgagaga tctaacgccg tcacctgtgc gtgacgctct
    29521 cgccagtggc ggctcatcca gcctggccca caccccgcgt ccagtacgtg ggtgtattta
    29581 cgctgtggga gcattgccag taagacgtca gcactccggc gctgtaactc tgcgtgttgc
    29641 tcatagtgcg cggctgcccg accaaatgcc gctgcaatgg cttgtttatt aaccgttgcc
    29701 atgcagcacc tccagcagac ggtcgatatc ctgcatttca tgcgctgcgg ttagcgttaa
    29761 gcgcagtcgc gcagtaccag cgggtacggt tggcgggcga atcgccgtga cccagcagcc
    29821 ttgctggcgc agtttttctg ccagttgtaa cgcacggctg ttatcaccga cgatcaatgg
    29881 ctggatggcg ctgcatgaat cagcaagcgt aaacggcaaa tcctgtactc cggcacgaaa
    29941 acgcgcaatg agtgacacca gtttttcgcg ccgtgcatcc ccctcatcac ggcgaatgac
    30001 cgccagcgac gcacgtaatg cctgcgcctg agcgggcggc atactggtgt tgtagataag
    30061 gtggcgggcg aattgcagca gataatccgc caccgtactg gagcaaagca ccgctgcccc
    30121 gctgacgcca aatcctttgc caaaagtcac caccagcaat tctggtttta ccttttgcag
    30181 ccagcagctg ccgcgcccct gctccccgat aacgcccgtg ccgtgggcat catcgaccat
    30241 caaccagcca ttgtgctgtt gcgttacctg ctggatttcc gccagtggcg cactatcgcc
    30301 gtccatgctg aacacgcctt ctgtcaccac cagttgctgc cccggacagg gggaagcaag
    30361 cagtcgcgcc agatgagtga catcgttatg aacaaaacgg cgaagctgcg acgggcttaa
    30421 actggcagcc tccagcaatg aggcatggct aagccggtca gcgacaatgc ggtcctcttt
    30481 tgccatcatc gcggcaatga ccgcctgatt agcggcaaaa ccagagataa atagcagtgc
    30541 ccgcgaatag cccagccact cggccagttc ttcttccagc gcctgatgcg caacgctata
    30601 accgctgacg tgaccggaac cgccgctacc gacgccaaat tgctccgcac cctgctgcca
    30661 ggcacggata atttgcggat gatggcttaa acctaaataa tcgttactgg aaaagttcag
    30721 atactggcga tcatccgcca ccagccagcg tccggctcct tgcgccactg gataacggcg
    30781 acgcagggca tcggcagcac gccgcgcatc gagcgccgcg ttgattttct cctgccagct
    30841 catagtgctg ccgcgttgta atattcgtca gtatccgggg tcatcagcgc ctgttcaaga
    30901 cgttgctgtt gttcgttatc ccctgccagc acggcagttt gctgcggatt tagccccagt
    30961 ttgcggaaca gttgcaggtc tttatcttct tccggattcg gcgtggtcag cagtttgcaa
    31021 ccgtagaaaa tcgagtttgc gcctgccata aagcacatcg cctgagtctg ttcgttcatc
    31081 tgctcgcgtc cggcagaaag gcgcacgtaa gaggtcggca tcatgatccg cgcgaccgca
    31141 atggtgcgaa taaaatcaaa ggcatcaacg tcatcgttat cggcaagcgg cgtgcctttc
    31201 accttcacca gcatgttgat tggcacgctt tccggcggcg tcggcaggtt tgccagttgc
    31261 agcaataatc cggcgcgatc ttttaccgtt tcgcctaagc ccacaatgcc gccagaacag
    31321 actttgatcc cggcatcgcg cactttttcc agcgtatcga ggcgttcctg ataagtgcgg
    31381 gtggtgatga tattgccgta aaactccggc gaggtgtcga ggttgtggtt gtagtaatcc
    31441 agcccggcgt tcgcgaggcg ctgcgcctga gattcactca acgtgcccag cgtcatacac
    31501 gcctccagcc ctaaatcttt taccccctgc accatctgct ccaggtacgg catatcgcgt
    31561 tcgttgggat tcttccacgc cgcgcccatg caaaaacgcg tcgatcccgc cgctttcgct
    31621 ttgcgcgccg actccagcac ctgttcaact tccatcaacc gctcggcttc cagaccggtt
    31681 ttatagcgcg agctttgcgg gcaatatttg caatcttccg ggcaagcacc ggttttaatc
    31741 gacagcaacg tgctgacctg cacctgacga ggatcgaaat gctggcgatg cacctgctgc
    31801 gcttcaaaca gcagatccag caacggtttt tcaaataatt ctgcgacttg cgacaatgtc
    31861 cagcgtgggc ggtgagccat ggggcttctc caaaacgtgt tttttgttgt taattcggtg
    31921 tagacttgta aacctaaatc ttttcaattt ggtttacaag tcgattatga caaaggacga
    31981 tcttgccttt gaccaacgcc atatctggca cccatacaca tccatgacct ccccgctgcc
    32041 ggtttatccg gtggtgagcg ccgaaggttg cgagctgatt ttgtctgacg gcagacgcct
    32101 gattgacggt atgtcgtcct ggtgggcggc gatccacggc tacaatcacc cgcagcttaa
    32161 tgcggcgatg aagtcgcaaa ttgatgccat gtcgcatgtg atgtttggcg gtatcaccca
    32221 tgcgccagcc attgagctgt gccgcaaact ggtggcgatg acgccgcaac cactggaatg
    32281 cgtttttctc gcggactccg gttccgtagc ggtggaagtg gcgatgaaaa tggcgttgca
    32341 gtactggcaa gccaaaggcg aagtgcgcca gcgttttctg accttccgca atggttatca
    32401 tggcgatacc tttggcgcga tgtcggtgtg cgatccggac aactcaatgc acaacctgtg
    32461 gaaaggctat ctgccagaaa acctgtttgc tcccgccccg caaagccgca tggatggtga
    32521 atgggatgaa cgcgatatgg ttggctttgc ccgcctgatg gcggcgcatc gtcatgaaat
    32581 cgcggcggtg atcattgaac cgattgtcca gggcgcaggc gggatgcgca tgtaccatcc
    32641 ggaatggtta aaacgaatcc gcaaaatgtg cgatcgcgaa ggtatcttgc tgattgccga
    32701 cgagatcgcc accggatttg gtcgtaccgg caaactgttt gcctgtgaat atgcagaaat
    32761 cgcgccggac attttgtgcc tcggtaaagc cttaaccggc ggcacaatga ccctttccgc
    32821 cacactcacc acgcgcgagg ttgcagaaac catcagtaac ggcgaagccg gctgctttat
    32881 gcacgggcca acttttatgg gcaatccgct ggcctgcgcg gctgcaaacg ccagcctggc
    32941 gattctcgaa tctggcgact ggcagcaaca ggtggcggct attgaagtac agctgcgcga
    33001 gcaacttgcc cccgcccgtg atgccgaaat ggttgccgat gtgcgcgtgc tgggggcaat
    33061 cggcgtagtc gaaacaactc gtccggtgaa tatggcggcg ctgcaaaaat tctttgtcga
    33121 acagggggta tggatccggc cttttggcaa actgatttac ctgatgccgc cctatattat
    33181 tctcccgcaa cagttgcagc gtctgaccgc agcggttaac cgtgcggtgc aggatgaaac
    33241 atttttttgc caataacggg aagtcagcgt gtgggtttct ggctacactt tctgcaaact
    33301 agaaaggagg gttcatgaaa ctcatcagta atgatctgcg cgatggcgat aagctgccgc
    33361 atcgtcatgt ctttaacggc atgggttacg atggcgataa tatttcaccg catctggcgt
    33421 gggatgatgt tcctgcggga acgaaaagtt ttgttgtcac ctgctacgac ccggatgcgc
    33481 caaccggctc cggctggtgg cactgggtag ttgttaattt acccgctgat acccgcgtat
    33541 taccgcaagg gtttggctct ggtctggtag caatgccaga cggcgttttg cagacgcgta
    33601 ccgattttgg taaaaccgga tacgatggcg cagcgccgcc gaaaggcgaa acccatcgct
    33661 acatttttac cgttcacgcg ctggatgtag agcatattga tgtcgatgaa ggagccagcg
    33721 gcgcgatggt cgggtttaac gttcatttcc actctctggc aagcgcatcg attaccgcga
    33781 tgtttagtta atcactctgc cagatggcgc aatgctatct ggtatcactt aaaggtatta
    33841 aaaacaactt tttgtctttt taccttcccg tttcgctcaa gttagtataa aaaagcaggc
    33901 ttcaacggat tcatttttct atttcatagc ccggagcaac ctgtgaacac attttcagtt
    33961 tcccgtctgg cgctggcatt ggcttttagc gtgacgctga ccgcctgtag ctcaacacca
    34021 cccgatcaac gtccttctga tcaaactgcg cctggtacct cttctcgccc gattctgtcg
    34081 gcaaaagaag cgcagaattt cgatgctcaa cactattttg catccctgac accaggtgct
    34141 gcagcgtgga atccttcccc gattaccctg cctgcgcaac ctgactttgt tgtcggcccg
    34201 gcgggcactc aaggtgtaac gcataccacg attcaggcgg cggtagatgc ggcaattatc
    34261 aagcgtacca acaagcgcca gtatattgcc gtgatgcctg gtgagtatca gggaacggtg
    34321 tatgtccctg ctgctccggg tggaattact ctgtacggta caggtgaaaa accgattgat
    34381 gtgaagattg ggctttccct tgatgggggc atgagccctg ccgactggcg tcacgacgtc
    34441 aacccgcgcg gcaaatatat gccaggtaaa ccagcgtggt atatgtacga tagctgccag
    34501 agcaaacgca gcgacagtat cggtgttctc tgctctgcgg tcttctggtc acaaaacaat
    34561 ggcctgcaac tgcaaaacct gaccatcgaa aacacgctgg gcgatagcgt agatgcaggt
    34621 aaccatccgg cggtggcact gcgtactgat ggtgaccagg tacagattaa caacgttaac
    34681 attctcggtc gtcagaacac cttctttgtc accaacagcg gtgtgcagaa ccgtctggaa
    34741 acgaatcgtc agccgcgtac gctggtgacc aacagctaca ttgaagggga tgtggatatc
    34801 gtttctggtc gcggcgcagt ggtgttcgat aacaccgaat tccgcgtggt gaactcacgt
    34861 actcagcaag aagcgtatgt gtttgcaccg gctacgctgt ccaacattta ctacggtttc
    34921 ctcgccgtaa acagccgttt caatgctttc ggtgatggtg tggcgcaact gggccgctcg
    34981 ctggatgttg atgccaatac caacggtcag gtggtgatcc gtgatagcgc catcaacgaa
    35041 ggttttaaca cggctaaacc gtgggccgat gcggtgatct ctaatcgtcc gtttgcgggt
    35101 aataccggca gcgtagatga taacgacgaa atacagcgca atctgaatga cactaactac
    35161 aaccgcatgt gggaatacaa taaccgcggc gtgggtagta aagtggttgc agaggcgaag
    35221 aagtaagagc aattaactat ttgccggatg cggcgtaaac gccttatccg gcctacggtt
    35281 cgatgcgatt tgtaggtcgg ataagatgcg caagcatcgc atccgacaat aagtgccgaa
    35341 tgcgacctac attcacatgg cgctttttac atctgacggt ttttattgaa gttaatcaaa
    35401 ctacccgcct tgataatctc gcgctcttca gcagtcagac tttccatata gagcgtaatt
    35461 tccgttaccg gcgcatcttc atggatcaca taacctttaa acgtcgtacc cggattatcc
    35521 agcgccgctt taatgccagg gatgtaaatg taatccccca cttcaaaggt tggcacttcc
    35581 gccatctgca gcggtaacat cccccagttg atgacgttag aacggtagcg tttagtcgcg
    35641 tactcttcgg caatattcgc cagaccgcca atcacacgct ggcagctcgc cgcctgttca
    35701 cgcgcagaac catcgcctgg tttcaccgca tataccatgc tgccaatttc agtttgcagc
    35761 ggatcaatat gctcctgacc agcaatctgc ttaatgcgcg caaacacctc tgtcagctcg
    35821 ctgacattcc ccgccagacg ctgattttcc agctcagccg tcgctttact tctgccaaca
    35881 taaccgggat cgcggcgtga cagggtaaac tccgccagac caatcggatt tgaacgataa
    35941 gaagaggttt caccggaagg aatcagttcg tcggtggtgg tcacttcgtc gaggatcttc
    36001 gagcacactt tcaggacgat attgtcagtc agcgcaccca attccggcca gtctttaatg
    36061 ttcggtccgt aaatcagcgg ttgctgagtt gcccctttca caaagccctg ataaacacgg
    36121 tttttatacg gcgttacatc gaaggcgtac tccggcacgt tgtcccagca atcaagttcg
    36181 ctggcagagg ttaaatagcc accgtttgcc gcagtcgcag cgatagaacg agcgtccatc
    36241 aacgccaccg ctgacatctg cccattagct ggcttagagc cttcgcggtt cgggaagtta
    36301 cgcgtggtgt ggcgaatact taatccgttg ttgattggcg tatcgcccgc gccaaagcat
    36361 gggccgcaga acgcggttct gatgattgcg cctgcgccaa tcaaatctgc taccacacct
    36421 tttttggcta aatccataaa caccggctgt gatgacgggt aaaccgccag cgagaaggtg
    36481 tcattgccac aggattgacc gcgcagtgca ttcgccgccg cgatgacgtt ttcgtagtta
    36541 ccgccagaac agcccgcgat aatcccctgc tgcactttca ggcgaccatt ttccacttta
    36601 tccagcagcg agagtttggc tttaccgtgc gccacgcgtt cggactcaat ttcaatctca
    36661 cgcagaatgt cggtcaggtt ctggttcagt gtgtcgattt catacacgtt gctcgggtgg
    36721 aacggcagcg caatcattgg tttgatggcg cttaaatcaa cgctgatgca gccatcgtag
    36781 tacgccatcg gttgagggtt aagctggcag taatcctggc cgcgaccgtg cagcgccagc
    36841 cagttatgca cttcttcatc agtttgccag acggagctta aacaggtggt ttccgtggtc
    36901 atcacgtcaa cgctgttacg gaaatcggta gagagcgcgc taacgcccgg tccaacgaac
    36961 tccatgactt tgtttttgac gtaaccgttt ttgaacaccg cgccaatgat agccagcgcc
    37021 acatcctgtg gccccacata cggcgcaggt tttccggtca gatgcaccgc aaccacgccc
    37081 ggatagtcga tatcccaggt gtcattaagc agctgtttta ccaactcacc gccgccctca
    37141 ccgactgcca ttgtccctaa tgcaccgtaa cgggtgtggc tgtctgaccc gaggatcatt
    37201 ttgcccccgc ctgccatcat ctcacgcata tattgatgga tgatcgcaat atgcggaggc
    37261 acaaaaatac cgccataacg ctgagctgcc gataaaccaa aaacatggtc atcaccgtta
    37321 atggtgccgc cgacggcgca gagtgagtta tggcagttgg tcagcacata cggcagcggg
    37381 aaacgttcca ttccggacgc tttagcggtc tgtacaatac caacaaaggt aatatcgtga
    37441 gaggctaatg aatcaaactt aattttaagt ttatccatat ttccggacgt attatgagaa
    37501 gagagaatag accaggcaat agtgcctttt ttggcttctt cttttttaat ttcgccggtg
    37561 aaatgttctt cggcaattat ttcgttatta ctggcgagaa acacgccttt
//
LOCUS       DAJTMC010000018        37310 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.018, whole genome shotgun sequence.
ACCESSION   DAJTMC010000018 DAJTMC010000000
VERSION     DAJTMC010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37310
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.018"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(<1..808)
                     /locus_tag="OO130_000817"
     CDS             complement(<1..808)
                     /locus_tag="OO130_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001019361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antA/AntB antirepressor family protein"
                     /protein_id="HCR5364326.1"
                     /translation="MTIEKSRFSSGAAPQPNVNTGVINGNDFAAIVPVIAGRIGGREA
                     NIVSAKALHKALGVGRDFTNWIKGRIDQYGFIAGTDFIRVENLSTPKRASAKTRQQIE
                     HDYLLSLDMAKEVAMVERNEQGRAIRRYFIQCEEELQRSVPEIAARYRRQLKARLSAA
                     NNFKPMCDALNMARAEMGKTTQQRHYSNESNMIARIVLGGMTAKQWAQANGITGEPRD
                     SMNAAQLEHLAYLESTNITLIDMGMEYEQRKGELTRLSQRWLAKRLEVNHA"
     gene            complement(822..1253)
                     /locus_tag="OO130_000818"
     CDS             complement(822..1253)
                     /locus_tag="OO130_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001437021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5364327.1"
                     /translation="MEKKNRPLQAANSDIRVSDVTPLTKSLQAPKRTPKKHRARVYML
                     RTGIEGWTENDILRYCRLSSGRNYASELERRLDIQLERIDEKNPDGIGSHLRYRFTGR
                     GDVLKVIRLVNHNADAGGYQGLSQPEIADILNLYPDNLTAA"
     gene            complement(1253..1456)
                     /locus_tag="OO130_000819"
     CDS             complement(1253..1456)
                     /locus_tag="OO130_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
                     /protein_id="HCR5364328.1"
                     /translation="MNIDTIPTKGYIRRFRLAELLGVSVSTIDRKVRNGSLPRPVKLG
                     EKITAFDAVEIHNWLAERRGKVA"
     gene            complement(1569..2417)
                     /locus_tag="OO130_000820"
     CDS             complement(1569..2417)
                     /locus_tag="OO130_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5364329.1"
                     /translation="MKKLPPAPFFRLNRAAELLEIDIDDLLIMAAAESIYLSAYLCGY
                     DGTLHSIASLYRNCSLFPDVKEDCLLLADIITEVEDEKSFKAGVVNHIDCHVLGLWHV
                     NASYILNIAGLDSIDTPARIFSPAHRKRELDVSANYYFFLDYTKHAFHEISVEDLYIT
                     RPWVEYIGECMDKNQPIYSPETRINLQALYEDVKNVEHGNRERHRQKREICLLALIYV
                     KRHYPEECKGKNGKETNEAWANATINHWALLGGGYDEPSIDYLKKLIADADRTPEERT
                     TAGKKR"
     gene            complement(2513..3772)
                     /locus_tag="OO130_000821"
     CDS             complement(2513..3772)
                     /locus_tag="OO130_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5364330.1"
                     /translation="MPKLTDMQIRAWIKSGERFEGRADGNGLYLRYREADKTPTWRFR
                     YKLAGKSRAMLIGSYSELSLSKARETAKELSARVALGYDVAGEKQKRKTEALAKMEAE
                     KNAMRVSELAAEYFERQILPRWKHPDILRRRIDKDINPCIGSMKVEDVKPRHIDDMLK
                     GIVDRGAPTIATDVLRWTRRIFDYGIKRHALEINPCSAFEVADAGGKEAARDRWLTRD
                     ELIQLFKAMRTAKGFSRQNEITFKLLLALCVRKMELCAARWEEFDLDGAVWHLPEERS
                     KNGDPIDIPLPSPAVEWLRELHTFSCNSAWVLPARKMQNRMIPHIQESTLPVALAKVR
                     AEMPDVPNFTIHDFRRTARTHLAALGVDPVVAERCLNHRIKGVEGIYNRHQYFDERKA
                     ALAQWADLLVALESGKDYNVTPLRRAN"
     gene            complement(3963..4826)
                     /locus_tag="OO130_000822"
     CDS             complement(3963..4826)
                     /locus_tag="OO130_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HCR5364331.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDISAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            4953..5669
                     /gene="rph"
                     /locus_tag="OO130_000823"
     CDS             4953..5669
                     /gene="rph"
                     /locus_tag="OO130_000823"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HCR5364332.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAVCDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            5735..6376
                     /gene="pyrE"
                     /locus_tag="OO130_000824"
     CDS             5735..6376
                     /gene="pyrE"
                     /locus_tag="OO130_000824"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HCR5364333.1"
                     /translation="MKPYQRQFIEFALGKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGLEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(6413..7009)
                     /gene="slmA"
                     /locus_tag="OO130_000825"
     CDS             complement(6413..7009)
                     /gene="slmA"
                     /locus_tag="OO130_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HCR5364334.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(7116..7574)
                     /gene="dut"
                     /locus_tag="OO130_000826"
     CDS             complement(7116..7574)
                     /gene="dut"
                     /locus_tag="OO130_000826"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HCR5364335.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            complement(7552..8772)
                     /gene="coaBC"
                     /locus_tag="OO130_000827"
     CDS             complement(7552..8772)
                     /gene="coaBC"
                     /locus_tag="OO130_000827"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HCR5364336.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEGLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSENNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            8944..9612
                     /gene="radC"
                     /locus_tag="OO130_000828"
     CDS             8944..9612
                     /gene="radC"
                     /locus_tag="OO130_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HCR5364337.1"
                     /translation="MKNNSQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            9829..10065
                     /gene="rpmB"
                     /locus_tag="OO130_000829"
     CDS             9829..10065
                     /gene="rpmB"
                     /locus_tag="OO130_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HCR5364338.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARSEKY"
     gene            10086..10253
                     /gene="rpmG"
                     /locus_tag="OO130_000830"
     CDS             10086..10253
                     /gene="rpmG"
                     /locus_tag="OO130_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCR5364339.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            10351..11160
                     /gene="mutM"
                     /locus_tag="OO130_000831"
     CDS             10351..11160
                     /gene="mutM"
                     /locus_tag="OO130_000831"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HCR5364340.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(11199..11678)
                     /gene="coaD"
                     /locus_tag="OO130_000832"
     CDS             complement(11199..11678)
                     /gene="coaD"
                     /locus_tag="OO130_000832"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HCR5364341.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(11686..12963)
                     /gene="waaA"
                     /locus_tag="OO130_000833"
     CDS             complement(11686..12963)
                     /gene="waaA"
                     /locus_tag="OO130_000833"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HCR5364342.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQVLLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            13376..14434
                     /gene="rfaQ"
                     /locus_tag="OO130_000834"
     CDS             13376..14434
                     /gene="rfaQ"
                     /locus_tag="OO130_000834"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HCR5364343.1"
                     /translation="MDKLFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLRVDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            14431..15555
                     /locus_tag="OO130_000835"
     CDS             14431..15555
                     /locus_tag="OO130_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCR5364344.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     YPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYHLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            15548..16345
                     /gene="rfaP"
                     /locus_tag="OO130_000836"
     CDS             15548..16345
                     /gene="rfaP"
                     /locus_tag="OO130_000836"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HCR5364345.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWSAIHRLRDVGVDTMYGVAFGEKGI
                     NPLSRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            16361..17377
                     /gene="waaO"
                     /locus_tag="OO130_000837"
                     /gene_synonym="rfaI"
     CDS             16361..17377
                     /gene="waaO"
                     /locus_tag="OO130_000837"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HCR5364346.1"
                     /translation="MSAHYFNPQEMINKTIIFDERPAASVASSFHVAYGIDKNFLFGC
                     GVSITSVLLHNNDVSFVFHVFIDDIPEADIQRLAQLAKSYRTCIQIHLVNCERLKALP
                     TTKNWSIAMYFRFVIADYFIDQQDKILYLDADIACQGNLKPLITMDLANNVAAVVTER
                     DANWWSLRGQSLQCNELEKGYFNSGVLLINTLAWAQESVSAKAMSMLADKAIVSRFTY
                     MDQDILNLILLGKVKFIDAKYNTQFSLNYELKKSFVCPINDETVLIHYVGPTKPWHYW
                     AGYPSAQPFIKAKEASPWKNEPLMRPVNSNYARYCAKHNFKQNKPINGIMNYIYYFYL
                     KIIK"
     gene            17394..18389
                     /locus_tag="OO130_000838"
     CDS             17394..18389
                     /locus_tag="OO130_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide 1,2-glucosyltransferase"
                     /protein_id="HCR5364347.1"
                     /translation="MNEFIKERFSYLADNKKENAPELNVSYGIDKNFLYGAGVSISSV
                     LINNSDINFVFHVFTDYVDDDYLKSFNETAKQFNTSIIVYLIDPKYFADLPTSQFWSY
                     ATYFRVLSFEYLSESISTLLYLDADVVCKGSLKPLTEIIFKDEFAAVIPDNDSTQAAC
                     AKRLNIPEMNGRYFNAGVIYVNLKKWHEANLTPYLLKLLRGETKYGSLKYLDQDALNI
                     AFNMNNIYLAKDFDTIYTLKNELYDRSHRKYQQTITDKTVLIHYTGITKPWHSWAGYP
                     SASYFNIAREQSPWKKYPLKEARTVAEMQKQYKHLFAHGEYIKGITSLIKYKLKK"
     gene            18399..19091
                     /gene="rfaY"
                     /locus_tag="OO130_000839"
     CDS             18399..19091
                     /gene="rfaY"
                     /locus_tag="OO130_000839"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HCR5364348.1"
                     /translation="MITSIRYRGFSFYYKDNDNKYKEIFDEILAYNFKTVKVLCNIDD
                     TKVSLIDTKYGRYVFKVFAPKTKRNERFLKSFVKGDYYQNLIVETDRVRSAGLTFPND
                     FYFLAERKIFNYASVFIMLIEYVEGVELNDMPIIPENVKAEIKASMEKLHALNMLSGD
                     PHRGNFIVSKDGVRIIDLSGKSCTAERKARDRLAMERHLGIANEIKDYGYYSVIYRTK
                     LRKFIKKLKGKA"
     gene            19117..20142
                     /locus_tag="OO130_000840"
     CDS             19117..20142
                     /locus_tag="OO130_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactose--(galactosyl) LPS
                     alpha1,2-galactosyltransferase"
                     /protein_id="HCR5364349.1"
                     /translation="MDLLAESITEVAVSGEIANTDRVLNIAYGIDRNFLFGAAVSMQS
                     VVMHNPDLAVKFHLFTDYIDEDYLQRVNAFTSKNANVEVIIYKVSNAFIDIFPSLKQW
                     SYATFFRLVAFQYLSETIENLLYIDADVICKGSLAGLLDINFDEDKFAAVIKDVPFMQ
                     EKPAKRLAIEGLPGNYFNAGVVYLQLEAWAKNDFMNKAIAMLASDPQHTKYKCLDQDI
                     LNILFFGHCNFISGDYDCFYGIDYELKNKSDEDYKKTITDDTKLIHYVGVTKPWNDWT
                     NYPCQKYFNEAYQASCWNDVAFIPATNEKQYQVKYQHAKKNGDTFNAFIYFIKFKLNK
                     YKRKLFG"
     gene            20227..21210
                     /locus_tag="OO130_000841"
     CDS             20227..21210
                     /locus_tag="OO130_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCR5364350.1"
                     /translation="MSNDYPLVSIIIPTYNSSDYITETLTKLEKQTYPNFEIVIVNDG
                     SKDNTSNVLREYGLTHSRLIIVNKENGGVSSARNTGIRKAQGQFICFMDDDDEIDPNY
                     LLKMYTRQHETGGDAIYCGLYGHYIKDGVTYSPINTEFNEGSLLFDFFYKKVRFHIGC
                     LFIRKQLLEENNLFFDEDLRLGEDLDFIYRLLITCDMYAVPYYMYKHNYRENSLMNSC
                     RTITHYRHESFAHERIYSSVMQLYKGNRKEEIHTLLSQNRAYHKTRYLWNVLLNGDFK
                     LLNQLVESNEKELRDCNLPGKRDKRRAKILASKNYILWRMVRLVNRKKNKR"
     gene            21256..22509
                     /locus_tag="OO130_000842"
     CDS             21256..22509
                     /locus_tag="OO130_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012311869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HCR5364351.1"
                     /translation="MSFCWNEINSGIKSLILILCIFSLMTLSLWDDVATKFLHAAGII
                     SALYFLATPKKTITNNPTLLIFISLCLLGIVNIIWYSHYKVSGSVYTNAYRGPMETGK
                     IALCSAFIFLVLFAKNEMRTKIKFGKLILFASLATQLLFFAHAMWQHFYLNVDRVALS
                     ASHATTAGYIILFPSLLASILILKSDFRHKTTLYTINFMLSLCAVIVTETRAAILVFP
                     FFALILIVMDSYINKRINYKLYCFIAIALLAGVFSFKDTLLMRMNDLNNDLVNYSHDN
                     TRTSVGARLAMYEVGLKTYSPIGQSLEKRAEKIHELEEKEPRLSGALPFVDSHLHNDL
                     IDTLSTRGIPGVVLTILAFSAILIYALRTAKEPYILILLFSLLVVGLSDVILFSKPVP
                     TAVFVTIILLCAYFKAQSDQYLLDK"
     gene            complement(22579..23550)
                     /gene="rfaC"
                     /locus_tag="OO130_000843"
     CDS             complement(22579..23550)
                     /gene="rfaC"
                     /locus_tag="OO130_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HCR5364352.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFDYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQMVCRAPGNELSQLTANAVKRFIEENAAMI"
     gene            complement(23554..24600)
                     /gene="rfaF"
                     /locus_tag="OO130_000844"
     CDS             complement(23554..24600)
                     /gene="rfaF"
                     /locus_tag="OO130_000844"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HCR5364353.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNTLLLQEEA"
     gene            complement(24610..25542)
                     /gene="rfaD"
                     /locus_tag="OO130_000845"
     CDS             complement(24610..25542)
                     /gene="rfaD"
                     /locus_tag="OO130_000845"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HCR5364354.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            25756..26952
                     /gene="kbl"
                     /locus_tag="OO130_000846"
     CDS             25756..26952
                     /gene="kbl"
                     /locus_tag="OO130_000846"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HCR5364355.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            26962..27987
                     /gene="tdh"
                     /locus_tag="OO130_000847"
     CDS             26962..27987
                     /gene="tdh"
                     /locus_tag="OO130_000847"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HCR5364356.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            28226..29260
                     /gene="waaH"
                     /locus_tag="OO130_000848"
                     /pseudo
     CDS             28226..29260
                     /gene="waaH"
                     /locus_tag="OO130_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
     gene            complement(29247..30206)
                     /locus_tag="OO130_000849"
     CDS             complement(29247..30206)
                     /locus_tag="OO130_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HCR5364357.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPLTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(30210..31493)
                     /gene="envC"
                     /locus_tag="OO130_000850"
     CDS             complement(30210..31493)
                     /gene="envC"
                     /locus_tag="OO130_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HCR5364358.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADE
                     RDQLKSIQADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQCGQRLQAYF
                     GYLNQARQETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            complement(31503..33047)
                     /gene="gpmM"
                     /locus_tag="OO130_000851"
                     /gene_synonym="pgmI"
     CDS             complement(31503..33047)
                     /gene="gpmM"
                     /locus_tag="OO130_000851"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HCR5364359.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            33292..33723
                     /locus_tag="OO130_000852"
     CDS             33292..33723
                     /locus_tag="OO130_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCR5364360.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            33865..34116
                     /gene="grxC"
                     /locus_tag="OO130_000853"
     CDS             33865..34116
                     /gene="grxC"
                     /locus_tag="OO130_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HCR5364361.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            34179..34646
                     /gene="secB"
                     /locus_tag="OO130_000854"
     CDS             34179..34646
                     /gene="secB"
                     /locus_tag="OO130_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HCR5364362.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            34646..35665
                     /gene="gpsA"
                     /locus_tag="OO130_000855"
     CDS             34646..35665
                     /gene="gpsA"
                     /locus_tag="OO130_000855"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HCR5364363.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGAEPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            35745..36566
                     /gene="cysE"
                     /locus_tag="OO130_000856"
     CDS             35745..36566
                     /gene="cysE"
                     /locus_tag="OO130_000856"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HCR5364364.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(36619..37092)
                     /gene="trmL"
                     /locus_tag="OO130_000857"
     CDS             complement(36619..37092)
                     /gene="trmL"
                     /locus_tag="OO130_000857"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HCR5364365.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
BASE COUNT         9246 a         8881 c         9297 g         9886 t
ORIGIN      
        1 acgcatggtt tacctccaga cgtttagcga gccagcgttg cgacaggcgg gtgagttctc
       61 ctttgcgctg ctcatattcc atgcccatat caattaacgt gatattggtg ctttcgagat
      121 aggcgaggtg ttcaagctgt gctgcgttca tgctgtcccg tggttcgcca gtaatgccat
      181 ttgcctgcgc ccactgcttc gccgtcatgc cacccagcac gatacgggcg atcatgttgc
      241 tctcgttgct gtagtggcgc tgctgcgtcg ttttgcccat ctcggcacgg gccatattca
      301 gcgcatcgca cattggctta aagttgtttg cggcactgag acgggctttt agctggcgac
      361 gatagcgggc ggcgatttca ggcacgctac gctgtaattc ttcctcgcac tggatgaaat
      421 aacggcggat agcgcggccc tgctcgttgc gttcaaccat tgccacttct ttagccatat
      481 ccagcgatag caggtaatca tgctctattt gctggcgagt ttttgcgctc gcccgttttg
      541 gcgtgctcaa attttcaaca cggataaaat cggttccggc aataaaaccg tactggtcaa
      601 ttcggccttt aatccagtta gtaaagtcgc ggcccacccc cagcgcttta tgcaacgcct
      661 tagcgctcac aatattggct tcacgcccgc caatgcgtcc ggcaataacg ggaacaatag
      721 cggcaaagtc gttgccgtta attactcccg tgttaacgtt gggttgcggg gcggcccccg
      781 aactgaatcg gcttttttcg atagtcatat tttcggctcc gttatgcggc ggtgagattg
      841 tccgggtata gattgagaat gtcggcaatt tcaggctgag aaagtccctg atagccgcca
      901 gcgtcggcgt tatggttcac aagccgaata acttttagaa cgtcaccgcg cccggtaaag
      961 cgatagcgta aatgtgaccc tatcccgtcg gggttctttt cgtctatacg ctcaagctga
     1021 atatcaagcc tgcgctctaa ctcgctggcg tagttccttc cagacgaaag gcggcaataa
     1081 cgcagaatgt cattttctgt ccatccctct ataccagtac gcagcatata gactctggca
     1141 cgatgctttt tcggtgtgcg ctttggtgct tgaagggatt ttgtaagggg cgtaacatca
     1201 gatacgcgaa tatctgaatt agccgcctgt aatgggcggt ttttcttttc catcaggcca
     1261 ccttcccgcg gcgttctgcc agccagttgt gaatctcaac cgcatcaaac gcagttattt
     1321 tttctcccag ctttaccgga cgtggtagtg aaccgtttct tactttacgg tcgatggtgg
     1381 atacactaac gcccagcaat tcagccaggc ggaagcgtct gatatagcct tttgtaggga
     1441 tagtgtcgat gttcatgatt cccgtaacct cttagcgata gttgttgctt cgttacgggt
     1501 atttaatggc gtgcacgctc aaaaaggaat gatcgtattt tttactatcg tgatagtaaa
     1561 atttactatc accttttttt ccctgccgtg gtgcgttctt ccggtgttct gtctgcgtcg
     1621 gctatgagtt tcttcaggta atctatggat ggctcatcat agccgccccc caaaagcgcc
     1681 cagtggttta ttgtggcgtt cgcccatgct tcgttggttt ccttcccgtt cttcccttta
     1741 cactcttctg ggtagtgccg cttcacataa atcaacgcca agaggcaaat ttcgcgcttt
     1801 tgcctgtggc gctctctgtt tccgtgctca acgtttttaa catcctcata aagagcttgt
     1861 aagtttatgc gagtttcggg gctgtatatg ggctggtttt tgtccatgca ttcgccgata
     1921 tattcaaccc acggccttgt tatgtacaaa tcctctacgg atatttcgtg gaaggcatgt
     1981 tttgtataat cgagaaaaaa ataataattc gcacttacgt ctagttctct ttttctgtgt
     2041 gcggggctga atatgcgtgc gggagtgtca atagaatcaa gccctgcgat gttgagtata
     2101 taggaggcgt taacatgcca tagcccaagc acatggcaat ctatatgatt tacaacgcct
     2161 gccttaaagc ttttttcgtc ctctacttca gtgattatgt cggcaagtaa taagcagtct
     2221 tctttgacgt ctgggaataa agagcaattg cggtaaagac tcgcgatgct atgtaatgtg
     2281 ccgtcgtagc cgcaaaggta cgcgctcaaa tatatagatt ctgccgccgc cataatcaaa
     2341 agatcatcaa tatctatttc taataattcg gctgctctgt taagcctgaa aaaaggggcg
     2401 ggtggtaatt ttttcattgt gtcacctttt acgctgcgcc ttattttgtg attgagccag
     2461 gcggtaaggc ttcccgcttt tcgccccgtc gagctaggct caatctattc agttagttcg
     2521 cccttctgag aggcgttacg ttgtagtctt ttccgctttc cagtgccacc agcagatcag
     2581 cccactgtgc cagtgctgct ttacgctcat caaaatactg atggcggtta taaatcccct
     2641 ctacgccctt aatgcgatga ttgaggcatc gttccgccac aacaggatca acacccaacg
     2701 ctgctaaatg agtacgtgcg gtgcgtcgaa agtcgtgaat cgtgaaatta ggcacatccg
     2761 gcatttcggc gcgaacctta gccagtgcta cgggtaaagt gctttcctga atatgtggga
     2821 tcattctgtt ttgcatcttc ctggccggaa gcacccatgc gctattacat gaaaaggtgt
     2881 gtagctctct caaccattca acggctgggg aaggtagagg tatatcaata gggtctccgt
     2941 ttttgctgcg ttcttccggc aaatgccata ccgcaccatc taaatcaaac tcttcccatc
     3001 gtgcggcgca taattccatt ttgcggacgc ataacgctaa cagtaatttg aacgtgattt
     3061 cgttctggcg actgaatccc ttagccgtgc gcatggcttt gaatagctgg attaactcat
     3121 cgcgggttaa ccagcggtca cgggcagctt ctttccctcc ggcgtctgcc acctcaaagg
     3181 ctgaacaggg gttaatctct agcgcgtgcc gtttgattcc gtagtcgaat atgcggcgcg
     3241 tccatctcag cacgtccgtt gctatggtcg gcgctccacg gtcaacaata cctttcagca
     3301 tgtcatcgat atggcgcggt ttcacgtcct ctaccttcat gctgccaatg caggggttta
     3361 tatctttgtc gatacggcgg cggagtatat cggggtgctt ccagcgcggg aggatctgac
     3421 gctcaaagta ttcagcggca agctctgaaa cgcgcatagc gttcttctct gcttccatct
     3481 tcgccagtgc ttcggttttg cgcttctgct tctctcctgc aacgtcatag cccagcgcaa
     3541 cgcgagccga tagctctttg gctgtctctc tggcctttga tagtgatagc tcgctatacg
     3601 aaccaattag catggcgcgg gacttccctg cgagtttata gcggaatctc catgtggggg
     3661 ttttgtcggc ttcacggtaa cgtaggtata aaccgttacc gtctgcccgc ccctcgaatc
     3721 gctctccgct cttaatccat gcgcggatct gcatgtctgt tagttttggc attttgcgaa
     3781 tatccaaact ttgaataggg ggtgtaccca aagcaatgta cccaaacctc attgggtaca
     3841 cctttgtgta caccataatc atgcgctttg ttgcgtcttt atgatagccc atgataacaa
     3901 aaaagcccgc tgggttgcgg gcttggtgtg atttatgcgt cgtcctaaat gttgctgata
     3961 gcttactcga tattctgaat ctgctcgcgc atctgctcaa tcaacacttt cagctcaatc
     4021 gcggagtttg tcacttcggc attgatagac ttcgacgcaa gagtgttcga ctcgcggttg
     4081 aattcctgca tcataaagtc cagacgacga ccaacggctt cttttttctt cagaatgttg
     4141 taggtctctt tgacatgcgc ttccaggcgg tccagttctt cggcaacgtc aattcgttgt
     4201 gccagcagaa ccagctcctg ctccagacgg ttgttttcca actgaacctg agcatcttcc
     4261 agcttcgcga ccagacgctc acgctgccat tgcaggattt ccggcatatg ggcgcggact
     4321 ttgaccactt cggcggtgac gccttccaga cgctgctcga tcaatgcttt cagtgcctga
     4381 ccttcggttt cgcgcgcgac aataaagtcg tccagcgtac catccagcgc cgcgagaatt
     4441 tcagcggcaa tggcgtcaag atcctgctcc tgggctgcca tcacgcccgg ccagcgtaga
     4501 atatcaaccg ggttgatttc cccttcgtca ctctgcattt ttacccagtt cgcggcagtt
     4561 accagctgtt tagccagttt ttcgttgagg atcagctcac cttgcgcgct aatatctggc
     4621 tcatagcgca gggtacattc cactttaccg cgcgtcaggc gagaacgaat acgctcgcga
     4681 acgacaggtt caaggctacg gaactgctcc ggcagacgaa agtaagtttc cagataacgc
     4741 tggtttaccg agcgcatttc ccaggttgcg ctcccccatt cacccttgat ttcacgccgg
     4801 gcgtaggcgg tcatactgcg gatcatagac gttcctgttt ataaaaggag aggtggaagg
     4861 attatagcca tcgatgcctt gtcaggatag gaataaccgc cggaagtccg tataatgcgc
     4921 agccacattt gtttcaagcc ggagatttca atatgcgtcc agcaggccgt agcaataatc
     4981 aggtgcgtcc cgttaccctg actcgtaact atacaaaaca tgcagaaggc tcggtgctgg
     5041 tcgaatttgg cgataccaaa gtgttgtgta ccgcctctat tgaagaaggc gtgccgcgct
     5101 tcctgaaagg tcagggccag ggctggatca ccgcagagta cggcatgctg ccacgttcta
     5161 cccacacccg taacgctcgt gaagcggcga aaggtaagca gggtggacgc acaatggaaa
     5221 tccagcgtct gatcgcccgt gctcttcgcg cggcagtaga tttgaaagcg ctgggtgagt
     5281 tcaccattac gctggactgc gacgtgcttc aggctgatgg tggcacgcgt accgcgtcga
     5341 ttacgggtgc ctgcgtggcg ctggcagatg cgctacagaa gctggtggaa aacggcaagc
     5401 tgaaaaccaa cccgatgaaa gggatggtag ccgcagtttc tgtcggaatt gtgaacggcg
     5461 aagcggtttg cgatctggaa tacgttgaag actctgccgc agagaccgac atgaacgtag
     5521 tgatgaccga agacgggcgc atcattgaag tgcaggggac ggcagaaggc gagccgttca
     5581 cccatgaaga gctactcacc ttgttggctc tggcccgagg gggaatcgaa tccattgtag
     5641 cgacgcagaa ggcggcgctg gcaaactgat ttttaaggcg actgatgagt cgcctttttt
     5701 ttgtctgtag aaaagtaaga tgaggagcga aggcatgaaa ccatatcagc gccagtttat
     5761 tgaatttgcg cttggcaagc aggtgttaaa gtttggcgag tttacgttga agtccgggcg
     5821 caaaagcccc tatttcttca acgccgggct gtttaatacc gggcgcgatc tggcactgtt
     5881 aggccgtttt tacgctgaag cgttggtgga ttccggcctt gagttcgatc tgctgtttgg
     5941 ccctgcttac aaagggatcc cgattgccac cactaccgcc gtggcgctgg cggagcatca
     6001 tgaccttgac ctgccgtact gctttaaccg caaagaggca aaagaccacg gtgaaggcgg
     6061 caatctggtt ggtagcgcgt tacaaggacg cgtaatgctg gtagatgatg tgatcaccgc
     6121 cggaacggcg attcgcgaat cgatggagat tattcaggct aatggcgcga cgcttgctgg
     6181 cgtgttgatt tcgcttgatc gtcaggaacg ggggcgcggc gagatttcgg cgattcagga
     6241 agttgagcgt gattacaact gcaaagtgat ctctatcatc accctgaaag acctgattgc
     6301 ttacctggaa gagaagccgg aaatggcgga acatctggcg gcggttaagg cctatcgcga
     6361 agagtttggc gtttaaagaa actcgccgga tgaaaagtca tccggcgtca tattactgca
     6421 actgtgccgc aattagcggc cagcgggcgt caaaatcatc cgtcgggcgg tatttaaatt
     6481 cgctgcggac aaaacgtgac agcatacctt cacagaaggc caggatctgg cttgccagca
     6541 gggtttcatc ggtggtgtaa ccttcaccct cacgcattct cttttcacgc aatacctgac
     6601 gcagctgcgc ttcaatacgc tcgaacagct ggttgatgcg cccttgcagg cgatcctgtt
     6661 caaacattag cgcatgacca gtgaggatgc gggtcaggcc aggattacgc tcaccaaaac
     6721 cgagaagcag caacacaatc agacgcaggc gcgctgtggt gtctttctca tctttcagaa
     6781 tcaggttgat gcgagtaatc aggctatctt cgataaactc aatcaggcta tcgaacatgc
     6841 gggtcttact ggggaagtgg cgatacagtg ccgcttcgga aacgccgaca gaggcggcca
     6901 gttttgccgt cgtgatacgt tggcttccat cgctggattc cagcatcagc gccagagact
     6961 gaagtatttc ctcgcgacgg ttccttttcg cagtttgttt ttctgccatg ttacaaaata
     7021 cccctgaaaa taagcacttg ccaggcggca cccacgctat gaccgcaaac gaaatgtttg
     7081 cggctatgtt atgacgttat tcggatgcgt atgtgttact gacgaccaga gtgaccaaag
     7141 ccgccttcac cgcggtcggt ggcgtcgaaa tcttccacca gattaaattc agcctgtact
     7201 accggaacaa aaatcatctg ggcgatgcgt tcgccaggtt gaatggtgaa gctgtcctga
     7261 ccacggttcc acacggaaat catcaactgg ccctgatagt cggaatcgat caatcctacc
     7321 aggttaccaa gcacgatacc gtgcttatgt cccaatccgg agcgcggcag catcattgcc
     7381 gccagtgaag gatcggcaat atgaatcgcc agcccggtcg gaaccagcgt agtgtcaccc
     7441 ggagccagtt ctacggcgtc gtcgagacag gcacgcaggt caagtccggc agagccagag
     7501 gtggcataag tcgggagcgg aaattccttc ccaacgcgcg ggtccagaat cttaacgtcg
     7561 atttttttca tcataacggg tcacgatctc gtcgagtaat agttggccaa ggagctcttt
     7621 gcgctcaagc ggtaagactt tatctccgtc ctgccagaaa aggtgtaatg cgttgttttc
     7681 gctgttaaat ccttgagttg gctgggaaac atcgttcgcg cagatcagat caaggttttt
     7741 acggatacgt ttttgccggg cgtattcttc cacattattt gtttcggcgg caaatccaac
     7801 gacgtagggt cgatggtctt ttagtgcggc aacgcctgcg acgatatcgg ggtttttaac
     7861 catttttatt gttaattcat caccctgcgt ggcctgcttt ttgatttttt ctggggccac
     7921 ggtagctgcg cgataatccg ccacggcggc gcagccgata aaaatatttt gctgctgtac
     7981 agaagcattc acggcggctt ccatttccag cgcggtcatc acatcaacac gtttaacaaa
     8041 cggtggcgtc ggtagtgaaa ccggacctga taccagcgtg acgttcgcgc cacgacgggc
     8101 agcggcggcg gcgatagcaa aacccatctt gccggagctg tgattagaga tataacgcac
     8161 gggatcgagc ggttcacgcg tcggaccggc ggtaatcata atgttcagat gtttcaggtc
     8221 gttgacgggc gaaaaatgcg ccaccgccat atccacaatg gttaacggat cgagcattcg
     8281 cccaggaccg atatcaccac aagcctgact gccactgtct ggcccccaga tgagcaaacc
     8341 acgggaagca agcccctcta aattatgctg cgtggcagcg gcacggtaca tctgctggtt
     8401 catggcgggg agcacggcta caggcgcagg tgtagccaga caaatcgtcg ataccaggtc
     8461 attcgccatt ccggcagcaa cacgggcaat caaatctgcc gtggcagggg cgagaatcac
     8521 taaatcagcc catttaccca gctcaatatg gcccatagcg gcttcggctg ccgggtccag
     8581 caaactgtcg gaaacgggat aaccagaaac cgcctgcaag ctaagtgggg tgataaaggc
     8641 ttttgccgct tcggtcatgg ctacgcggac gtcggccccg cgatcgcgca aacgacgcac
     8701 cagttcaggg gttttatagg cagcaatacc gccgctaacg ccgagaacga tttttttacc
     8761 ggccaggctc atgatgattt ttcctgttgg gtgacaccag aagttggcga ttttatcaca
     8821 atacttttgt tgtcgtgcct tcacctgaga atcactttgc gaggcgcttt ccaggattga
     8881 aaactggccg tcgatttaac ggaacggcta tgacaggatg cgagcaccac aaaggaggtg
     8941 aaggtgaaaa acaattcaca gctgttgatg ccgcgcgaaa aaatgctgaa gtttggtatt
     9001 agcgccttaa cggatgtcga gctgctggcg ctatttctgc gtaccggaac gcgcggtaaa
     9061 gatgtattaa ccctggcaaa agagatgctg gagaatttcg gctctcttta tggcttgtta
     9121 acctctgaat atgaacaatt tagtggcgtt catggaattg gcgtggcgaa atttgcccag
     9181 ttaaagggga ttgctgaact ggcgcggcgt tactacaacg tgcggatgcg tgaagaaagc
     9241 cctttactca gcccggagat gacgcgggaa tttttacaaa gccagcttac gggtgaggag
     9301 cgggagatct ttatggtgat ctttctcgac tcacaacacc gggttataac gcatagccgt
     9361 cttttttccg gcacgctaaa ccatgttgaa gtccatcctc gggaaattat ccgcgaagcg
     9421 ataaaaataa acgcctcggc gctgatcctt gcacataatc acccttcggg ttgtgctgaa
     9481 cccagtaaag cggataaact cattactgaa cggataataa agagttgtca gttcatggat
     9541 ttacgcgtgc tcgaccatat cgtgattggg cgtggagagt atgtttcttt tgccgaacgc
     9601 ggctggattt aacccgctat gcgcgatcct tcgggatctt tgtctgttcg ggacttgagc
     9661 acatcgctga gtcagcgtat actacgccac ctttgagaat ctcgggtttg gcatttgggc
     9721 ctggcaatcg agagttcaca tagaactgcg atgactgggc tgtaaagcct gacgaggcgc
     9781 caatacccca tacgaagctc gagctaattt gatttttgga gaatagacat gtcccgagtc
     9841 tgccaagtta ctggcaagcg tccggtgacc ggtaacaacc gttcccacgc actgaacgcg
     9901 actaaacgcc gtttcctgcc gaacctgcac tctcaccgtt tctgggttga gagcgagaag
     9961 cgttttgtca ccctgcgcgt atctgctaaa ggtatgcgtg taatcgataa aaaaggcatc
    10021 gatacagttc tggctgaact gcgtgcccgt agcgaaaagt actaagtact tagaggaaat
    10081 aaatcatggc taaaggtatt cgtgagaaaa tcaagctggt ttcttctgct ggtactggtc
    10141 acttctatac cactacgaag aacaaacgta ctaagccgga aaaactggaa ctgaaaaaat
    10201 tcgatccagt tgttcgccag cacgtgatct acaaagaagc gaaaatcaaa taattctcgc
    10261 tttgatgtaa caaaaaacct cgctccggcg gggttttttg ttatctgctt gcccccatat
    10321 tgactgcatc tgttcattcc tggagatgct atgcctgaat tacccgaagt tgaaaccagc
    10381 cgccgcggca tagaaccgca tctcgttggt gcaaccattc ttcatgcagt ggtgcgcaac
    10441 ggacgcttgc gctggccggt ttcagaagag atctaccgtt taagcgacca accagtgctt
    10501 agcgtgcagc ggcgggctaa atatctgctg ctggagctgc ctgagggctg gattatcatt
    10561 catttaggga tgtctggcag cctgcgcatc cttccagaag aacttccccc tgaaaagcat
    10621 gaccatgtgg atttggtgat gagcaacggc aaagtgctgc gctacaccga tccgcgccgc
    10681 tttggtgcct ggctgtggac caaagagctg gaagggcata atgtgctggc ccatcttgga
    10741 ccggagccgc ttagcgacga tttcaatggt gagtatctgc atcagaagtg cgcgaagaaa
    10801 aaaacggcga ttaaaccgtg gctgatggat aacaagctgg tggtaggggt agggaatatc
    10861 tatgccagcg aatcactgtt tgcggcgggg atccatccgg atcggctggc gtcatcactg
    10921 tcgctggcag agtgtgaatt gttagctcgg gtgattaaag cggtgttgct gcgttcgatt
    10981 gagcagggtg gtacaacgct gaaagatttt ctgcaaagtg atggtaaacc gggctatttc
    11041 gctcaggaat tgcaggttta cgggcgaaaa ggtgagccgt gtcgggtgtg cggtacgccg
    11101 attgtggcga ctaaacatgc gcagcgggca acgttttatt gtcggcagtg ccagaagtaa
    11161 ttcatgcgcg ccggatcgca taccatccgg cataaacgct acgctaactt cgccatcagc
    11221 gcctgatgga cattctccgg caggaaatgg gtgacatcgc cctgatggcg cgccacctct
    11281 ttcaccaacg atgaagagat aaacgaccac tctttcgacg gcatcagaaa cacactttcc
    11341 agttccggca ttaagtggcg attcatatgc gccagctgca tttcatattc aaaatccgcc
    11401 accgcacgta ggccacgaat cagcaccgta gcgtgttgat tacgggcgaa gttcgccatc
    11461 aaatcactaa acccaactac ttccacgttc cccagatgcg cggttgcctg ctgtgccagc
    11521 gccacacgct cttccagggt aaacatcggt tttttactgg ggctggcggc aatcgccaga
    11581 ataacgtgat cgaacatttg cgtggcgcgc gtcacgatat cgatatgacc attggtaatg
    11641 ggatcgaaag tacccggata aatcgcccgt ttttgcataa caacctcaat gcgttttcgg
    11701 tggcaggtaa ggttccagca gttgaagcag gcgctgtagc gcgccctggt tttgatacag
    11761 tacttcaacg gcatgacggc catagaaact acggtaatcg gcgtcggtga gtaaagagga
    11821 aacctctttt gcaagcgtag tggcatcggt aacggtaatc agcccgcttg cctgctccag
    11881 ccgcgcgcaa atgtctttaa aattaaaagt atgcggcccc atcaataccg gaatagcgtg
    11941 tgcggcagct tccagcggat tatgcccacc acgttcaacc agtgaaccgc caacaaaggc
    12001 gagatcggca atgccgtaca gtaacatcaa ctcgcccatc gtatcgccaa ccacaacctg
    12061 cgtgctggtg gaggggactt cccctgaaga gcgtgtgata tagcttagtc cagcctggcg
    12121 gacaaggtta atcgcatccg ggaagcgttc tggatgacgg ggtaccagga tgagcaataa
    12181 attcgggaat tgctgtaaca atacctgatg tgcggcgatc accacactct cttcaccttc
    12241 gtgagtgctg gtggcaatcc ataccgggcg gtgtggtgcc cactggcggc gcagcgtcac
    12301 ggctttagca gccaactgcg gcgttacaga aatatcgaat ttcaggctac cggtaacggt
    12361 tacctggtta ttttttgcgc ccagcgccac aaaacgtgca ccatcttctt cattttgggc
    12421 ggcaatcagc gtaatacgac gcagcaagcg acggacgaat ttacccagtt tggcataacc
    12481 tgcggccgag cgggcagaga gtcgcgcgtt cgcgatcacc agcggaattt tacgtttatg
    12541 tagcgccgca atcaggttag gccatagttc ggtttccata atcaacacca gtttagggtc
    12601 gactttattc aggaaacgat taagtgcatc gggcaggtca tacggcagat aaacgtgctg
    12661 aacatccttc ccgaaagccg attgtacgcg ctccgaaccg gttggcgtca tggttgtaac
    12721 ggtaatcggt aaatcaggat aacgatgacg cagcgcgcgc accaacggga tcgccgccag
    12781 agtttcaccg acggagacgg agtgcagcat aatgccgcct ggttttagcg gatggcggta
    12841 aaaaccgtaa cgttcacccc agcgttttcg ataggccgga gccttacgtc cgcgcaccca
    12901 gagccgtatc cagatcagcg gctgaataag gtagagaagg gcggtgtaaa gcaattcgag
    12961 catagtaaat agctgactta tggatgtgct ggggattcta tgtatttagc tgtggcttta
    13021 ccattacttt tcccgttttt gacttaaata gcaatttacc cgccaattct tgctgaacat
    13081 ctatactttt tttgtatttt tatgcgattc attgatttct gctgtacgtg caggaaggaa
    13141 tcgcagagca acagtgaaag aatatggttg ctaatgattt tatttgattg atttctttat
    13201 tgaaaatcaa caatatgctt aaaaatcatt cgggtcggat tgccgcgaaa gaagtgatac
    13261 actagcgcgt caatatcagt gcggtatcag tattcaggta gctgttgagc ctggggcggt
    13321 agcgtgcttt ttctgcttca ccttaatcgg ataatctcaa caaaaagagt tacttgtgga
    13381 taagctattt cgaagaattt tgctcattaa gatgcgtttt catggggata tgttattaac
    13441 tactcccgtc attagttcgc tgaaaaaaaa ttaccctgac gcaaaaatcg atgtgctgct
    13501 ttatcaggac accatcccga tcctgtctga aaatccagag attaacgcgc tctacggcat
    13561 aaaaaataaa aaagcaaaag cctcagaaaa aattgccaac ttttttcatc tcatcaaggt
    13621 attacgtgcc aataagtatg accttatcgt caatctcacc gatcaatgga tggttgctat
    13681 actggttcgc ttattaaatg cccgtgtgaa aatttcccag gattatcatc atcggcagtc
    13741 tgctttttgg cgtaaaagtt tcacccattt ggtgccgttg cagggtggaa atgtggtgga
    13801 aagtaactta tccgtgctga ccccattgcg agttgattcg ttggtgaagc agacaaccat
    13861 gagttacccg cctgcaagct ggaaacgtat gcgtcgcgaa cttgatcatg ctggtgttgg
    13921 acaaaattat gtggttatcc aacctacggc gcggcaaatc ttcaaatgct gggacaacgc
    13981 caagttttcc gctgtgattg atgccttaca tgctcgtggt tatgaagttg ttctgacgtc
    14041 cggcccagat aaagacgatc tggcctgcgt caatgaaatt gcgcagggat gccagacgcc
    14101 accagtaacg gcgctggctg gaaaggtgac cttcccggaa cttggtgcgt taatcgatca
    14161 tgcgcagctg tttattggcg ttgattccgc accggcgcat attgccgctg cagttaatac
    14221 gccgctgata tcgctgtttg gtgcgacaga ccatattttc tggcgtccct ggtcaaataa
    14281 catgattcaa ttctgggcgg gagattaccg ggaaatgcca acgcgcgatc agcgtgaccg
    14341 aaatgagatg tatctttcgg ttattccggc ggcagatgtc attgctgctg tcgataaatt
    14401 actgccctcc tccacgacag gtacgtcgtt atgatcgttg ctttttgttt atataaatat
    14461 tttccctttg gcggtttgca gcgcgatttt atgcgtattg ctcagacagt cgccgcccga
    14521 ggtcatcatg ttcgggttta tacccagtcg tgggaaggcg aataccctga tgtatttgaa
    14581 ctgatcaaag tgccggttaa atcgcatacc aatcacgggc gcaatgcgga gtattttgcc
    14641 tgggtgcaaa aacatttacg cgaacatccc gtcgataaag tcgttggatt caacaaaatg
    14701 ccggggctgg acgtttatta tgccgctgat gtttgttatg ccgagaaagt cgcgcaggaa
    14761 aaaggctttt tctatcacct gacgtcacgt tatcgccatt atgccgcctt tgagcgggca
    14821 accttcgaac agggcaagcc gacacagctg ctgatgctga cagataagca aatcgccgat
    14881 ttccagaaac attatcagac tgaagcggag cgttttcata ttctgccacc ggggatttat
    14941 cctgatcgta aatatagcca gcagccagct aatagccgtg aaatcttccg taagaagaat
    15001 ggaataaccg aacaacaata tttattgttg caggtcggtt cagacttcac gcgtaaaggt
    15061 gtcgatcgtt ccattgaagc acttgcttcg ttaccggatt cgctgcgcca caacacattg
    15121 ctatatgttg ttggacagga taaaccgcga aaatttgagg cgctggcaga aaaacgcggc
    15181 gtgcgcagta atgttcactt cttctcgggg cgcaacgatg tctcggaatt aatggcggcg
    15241 gcggatttat tactgcatcc tgcctaccag gaagcagcgg gaattgtgct actggaagcg
    15301 attactgcag gattaccggt actaacaaca gccgtttgtg gctatgcgca ttatattgtc
    15361 gacgccaatt gcggcgaggc tattgctgag ccattccgcc aggaaacatt gaatgagatt
    15421 ttacgcaaag cgttaacgca atcttcattg cgccaggcct gggcggaaaa tgcgcgacat
    15481 tatgctgata cacaagattt atacagtctg ccagagaaag cggcggatat cataacgggt
    15541 ggtctggatg gttgaactta aagagccgtt tgccacgtta tggcgcggta aagatccttt
    15601 tgaggaagtt aaaaccttgc agggtgaggt ttttcgtgaa ctggaaactc gccgcactct
    15661 gcgatttgaa atggcgggca aaagctattt tctcaaatgg catcgcggca cgaccctgaa
    15721 agagataatc aaaaatttac tctcattgcg gatgccagta ttaggcgcag accgcgaatg
    15781 gagtgcgatt catcgactgc gggatgtcgg cgttgatact atgtatgggg tggcatttgg
    15841 cgaaaaaggt attaatccgc tgagcagaac ctcgtttatt attaccgaag atctgacccc
    15901 aaccatcagt ctggaagatt actgtgctga ctgggcgacc aacccaccag atgttcgcgt
    15961 aaagcgtatg cttattaagc gtgtcgcgac gatggtgcgc gatatgcatg ctgcgggcat
    16021 taaccaccgt gactgttata tctgtcattt cctgctgcac ttgccttttt ccggtaagga
    16081 agaggagtta aaaatttcgg taattgacct acaccgggcg cagcttcgca cgcgcgttcc
    16141 acgtcgttgg cgcgataaag atcttatagg gctttatttt tcttcaatga atattggcct
    16201 gactcagcgg gatatctggc ggtttatgaa agtgtatttt gccgccccgc ttaaagacat
    16261 tctcaagcag gaacaaggac tgctgtcgca agcagaagca aaagccacaa aaatcaggga
    16321 aagaacgatt cgaaaatcgt tgtaatttcc ggagagaaat atgagtgccc actattttaa
    16381 tccacaagag atgatcaata agacaatcat cttcgatgaa aggccagcgg cgtcagtggc
    16441 atcatcattc catgttgctt atggcattga taaaaacttt ctttttggtt gtggtgtttc
    16501 aatcacgtca gttttgttac ataacaacga cgtgagtttt gttttccacg tttttattga
    16561 tgatatccct gaagccgata tccagcgttt agcccaattg gcgaaaagct atcgtacctg
    16621 tatccagatc catctagtaa attgtgaacg gcttaaggca ttaccgacga ccaaaaattg
    16681 gtctattgcc atgtatttcc gttttgtaat tgcagattac tttattgatc aacaagataa
    16741 gatcctttac ctggatgctg atatcgcctg tcagggaaac ttaaagccgc tgataacaat
    16801 ggatcttgcc aataacgttg ctgctgttgt tactgaacgc gatgctaact ggtggtcgtt
    16861 acggggtcaa agtctgcagt gtaatgaact tgaaaagggc tactttaatt caggtgtcct
    16921 gttaattaat acactagcgt gggcgcagga gtccgtttct gctaaagcga tgtcgatgct
    16981 tgctgataaa gccatcgttt cccgtttcac ctatatggat caagatatcc ttaatcttat
    17041 cctgttaggg aaagttaaat tcattgatgc taaatacaat acgcaattta gtttaaatta
    17101 tgaattaaaa aaatcatttg tttgtccaat taatgatgaa accgtattaa ttcattatgt
    17161 cggcccgaca aaaccctggc attactgggc cggttatcca agtgcgcaac cttttatcaa
    17221 agccaaagaa gcatcgccct ggaaaaatga accgttaatg cggccagtta actcaaacta
    17281 tgctcgttat tgcgccaagc ataattttaa acaaaataaa ccaattaacg ggataatgaa
    17341 ttatatttat tatttttatt taaagataat aaaatgaaat tgagaaaagt taaatgaatg
    17401 aatttataaa agaacggttt tcgtatttag cagataataa aaaagaaaac gccccagagc
    17461 taaatgtttc ctacggtatc gataagaatt ttttgtatgg tgctggcgtt tcaatttctt
    17521 ccgttttgat taataattca gatattaatt ttgtctttca tgttttcact gattatgtgg
    17581 atgatgatta tttaaagtca tttaatgaaa cagcaaaaca atttaatacc tcaattattg
    17641 tatatttaat tgaccccaaa tactttgctg atctgccgac gtcacagttt tggtcatacg
    17701 cgacatactt cagggtattg tcttttgaat atctgagtga aagtatttcc acactgctgt
    17761 atctggatgc cgatgttgtt tgtaaaggaa gcctgaaacc tctcacagaa attatattta
    17821 aagatgagtt tgctgcggtc attcctgaca atgatagtac tcaggcggca tgtgcaaaac
    17881 gcctcaacat tcccgaaatg aatggacgtt atttcaatgc aggcgttatc tatgtcaatc
    17941 ttaaaaaatg gcatgaagca aatttgacac cgtatttact caaactttta cgaggggaaa
    18001 ctaaatatgg ctctcttaaa tatttagatc aggatgcgtt gaatatcgca tttaatatga
    18061 ataatatcta ccttgcgaag gattttgata ctatttatac cctgaaaaac gaactttatg
    18121 atcgtagtca tcgaaagtat cagcaaacca ttaccgataa aacagtgttg attcactata
    18181 cagggataac taaaccatgg catagctggg ctggatatcc gtctgcatca tactttaata
    18241 tcgcgcgtga acaatctccc tggaagaaat atcctcttaa agaggcgcgg actgttgcag
    18301 aaatgcagaa acaatataag catctgtttg cccatggtga gtatataaaa ggcataactt
    18361 cattaattaa gtacaagctt aagaaataaa ttactcttat gattacaagt atacgttatc
    18421 gcggcttctc attttattac aaagataatg ataataaata taaagaaatc tttgatgaaa
    18481 tactggccta taattttaaa acagtaaaag tattatgtaa tattgatgat accaaagtat
    18541 cgttgattga tacgaaatat ggtcgctatg tttttaaagt ctttgctccg aaaacgaaaa
    18601 ggaatgaacg ttttttaaaa tcttttgtaa aaggtgacta ctatcagaat ttgatcgttg
    18661 aaaccgatcg tgtcagaagt gcaggtctta cttttcctaa tgatttttat tttctggctg
    18721 agcgtaaaat tttcaactat gccagtgttt ttatcatgct tatcgagtat gtcgagggcg
    18781 tagagctcaa tgatatgccg attattccag aaaatgttaa agcagaaatt aaggcttcga
    18841 tggaaaaatt gcatgcctta aatatgctat cgggcgaccc acaccggggt aactttatcg
    18901 ttagtaaaga tggtgtcagg ataattgacc tatccggtaa aagctgtacg gcagagcgta
    18961 aagctcgtga tcgtctggcc atggaacgtc acctgggtat tgccaacgaa ataaaagatt
    19021 atggctacta ttcagtaatc tacagaacca aactacgcaa atttattaaa aaattaaaag
    19081 gcaaagcgta aaccacacag tcaaaacgga accaacatgg atttattagc agagagtatt
    19141 actgaagtcg ctgtctctgg ggaaattgct aacaccgatc gtgtgttaaa tatcgcttac
    19201 ggtattgacc gtaacttttt atttggtgcg gcagtatcta tgcaatcagt tgttatgcat
    19261 aacccggacc ttgcggttaa gtttcatctc tttaccgact acattgatga agattatcta
    19321 caacgtgtta atgcttttac cagcaaaaat gctaacgttg aagtaataat ttataaagtc
    19381 tccaatgcct ttattgatat cttccccagc ctgaaacagt ggtcttatgc aacattcttc
    19441 cgtttagttg cgttccagta tttgagtgaa actattgaaa atctgttata tatcgatgct
    19501 gatgtcatct gtaaaggctc attagctgga ttgcttgata ttaattttga tgaggataag
    19561 ttcgcagctg ttattaaaga tgtgcctttt atgcaggaaa aaccagcgaa gcgtctggct
    19621 atagagggac ttccagggaa ttatttcaac gccggtgtag tatatctgca gcttgaagca
    19681 tgggcgaaaa atgattttat gaataaagcc attgctatgc tggcaagtga cccgcagcac
    19741 acgaagtata aatgccttga tcaggatatt ttaaatattc tgttctttgg tcattgtaat
    19801 tttattagcg gcgattatga ttgcttttat ggcattgact atgagttaaa aaataaaagc
    19861 gatgaagatt ataaaaagac cattaccgat gatactaagc tgattcatta tgttggcgta
    19921 acgaagccct ggaacgactg gacgaattat ccctgccaga agtattttaa tgaggcttat
    19981 caggcttctt gctggaatga tgtggcgttt attccagcca cgaatgaaaa gcagtatcaa
    20041 gtgaaatatc aacatgcaaa gaaaaatggt gatacgttta acgcttttat ttacttcatt
    20101 aaatttaaat taaataagta taaaagaaaa ctatttggat aataataaaa atcatggatg
    20161 tctgagttac aataataatt aaggatgagt actatcatat tttattagtt caggcaagag
    20221 aacacgatga gcaatgatta ccctttagta tctataataa taccgacgta taattcatct
    20281 gattacatta ctgaaactct aacgaaatta gaaaaacaaa cttacccaaa ttttgaaatt
    20341 gttattgtta atgatggttc taaagataac acatcaaacg ttttgagaga gtatgggtta
    20401 acccactctc gattaattat tgtcaataaa gaaaatggcg gtgtttcgtc tgccaggaat
    20461 acaggtatcc gcaaggcgca aggacagttt atatgtttta tggatgatga tgatgagata
    20521 gatcctaact atctgctgaa gatgtatacc agacaacatg agacgggagg ggatgcgatt
    20581 tattgtgggc tttatggcca ttatataaaa gatggtgtta cttactcacc tataaataca
    20641 gagtttaatg aaggatcttt acttttcgac tttttttata aaaaggttag attccatata
    20701 gggtgcttgt ttataagaaa acaacttctg gaagagaata atcttttttt tgatgaagat
    20761 ttacgactag gagaagatct tgattttatc tatcgactgc taattacatg cgatatgtat
    20821 gcggttccat attatatgta taagcataac tatagagaaa attccttaat gaactcatgt
    20881 agaaccatca ctcattatcg acatgagtca tttgcgcacg aaagaatcta ctcttctgtg
    20941 atgcagttat acaaaggtaa ccggaaagaa gaaattcata cattattgag tcaaaataga
    21001 gcttatcata aaactcgtta tttatggaat gttctactta atggtgattt taaactattg
    21061 aatcaattag ttgaaagcaa tgaaaaagaa ttaagagatt gtaatcttcc tggcaagaga
    21121 gataagagac gagcaaaaat attagcatca aaaaattata ttctctggag gatggtaaga
    21181 ctggtaaata gaaaaaagaa taaacgttag atcttgcatt aaataaccca tcctcgtagc
    21241 ataggttgaa attatatgtc gttttgttgg aatgaaatta attctggtat aaagtcttta
    21301 attcttatat tatgtatttt ttctttaatg actttgtctt tatgggatga tgttgcaaca
    21361 aagtttcttc atgcagctgg aattatatct gcattgtatt ttcttgcgac accaaaaaaa
    21421 acaataacta ataatcctac tttgttaatt ttcatctcat tatgtctttt gggtatcgta
    21481 aatatcatct ggtattcaca ttataaagtt tcaggctctg tttataccaa tgcatatcgt
    21541 ggcccaatgg aaactggaaa aattgccttg tgtagcgcct ttattttctt agttcttttt
    21601 gctaaaaatg agatgagaac aaaaataaaa tttgggaaac taattctgtt cgcatccctg
    21661 gcaacgcagt tacttttttt tgcgcatgcc atgtggcaac atttctattt aaacgtcgac
    21721 cgtgttgcat tatcagcttc ccacgctaca acagcaggct acatcatcct ttttccttct
    21781 ttactggcat caattctcat tttaaaatcc gactttagac ataaaacaac attatataca
    21841 attaacttca tgcttagctt atgtgctgtc atagtaactg agacgcgtgc agccatatta
    21901 gtgtttccat tctttgcgtt aatattaatc gtaatggata gttatattaa taagcgaatt
    21961 aattataagt tatattgttt tattgcgatt gcattattag caggtgtatt ttcttttaaa
    22021 gatacattgc ttatgagaat gaatgactta aataacgatt tagttaatta ttcgcatgat
    22081 aacaccagaa cttcagtcgg tgcccgtctg gcaatgtatg aagttggctt aaaaacatat
    22141 tctccaatag gacaatcact ggaaaaacgt gcagaaaaaa tacatgagct agaagaaaaa
    22201 gagcctagat tgagtggcgc tttacctttt gtagattctc atttgcataa cgatctcata
    22261 gatacgttat caacgcgtgg tattcctgga gttgtattaa caattttagc attttcagca
    22321 atactcatat atgccttaag aactgctaaa gaaccttata ttttaatctt gcttttttca
    22381 ctactggtag taggcctaag tgatgtaata ctcttttcta aaccggttcc gactgctgtg
    22441 tttgtcacca taatattgct ttgtgcttat tttaaagcac aatcagacca atatttatta
    22501 gataagtaat gaatatatga aataaaatca gcaaggaggc tgattttatt tcaaaattat
    22561 ctattaaggg gttgtaattt aaatcatagc agcgttttct tcaatgaatc gcttaacagc
    22621 atttgctgtc aattgagaca actcattccc cggagcccta caaaccattt gattcttccc
    22681 atacccacca attaatcccg gatcggttgg tccataaacc gtgatattgg gtctatccag
    22741 tgccgccgtt aaatggctta accccgtatc caccgacact acaaatttag ccccggccag
    22801 cacgcgggca acgccttcca gactcatctt cggcaatact tcaacataat caaatccttc
    22861 cgccagtcgt ttcgcccgtt cttcctcatg cggcgcgccc cacggaagtt taatccgtat
    22921 tcctgaatca gccagtaaac caatcaattc tcgccagtgt tcttccggcc agtgtttatc
    22981 atcacgggtc gtcgcatgaa gaaatacggc atattcgcca gcatctgtag gcaggttcgt
    23041 cagaaaatgc tgtgcgatag cataatcgcc ctgggtttgc ggtttgctat agcccaaact
    23101 tttggcaaac agttcgcggg tgcgttctac ggcgtgctgc tgttttgcaa tatgatgctt
    23161 acaattgtaa aacaggctgg ctaaaggttc gcgagcggtt tgccagtcca atccatgctt
    23221 tacgccatgc gccagacgtg tcaccagtgc cgcgcttttt accagcccct gagcgtcgat
    23281 aactgcgtca tagttctctg cttgtagcgc ttcacgaaac gctttgcgtt cagcttttat
    23341 gggggccgag aaccaggctt tacgccagcg acgtattgcc acaggaataa ctcgctcaac
    23401 ggcagcgtgc caggaaggaa tctgtgcgaa cccttcttcc accacccagt caaacttaat
    23461 ccctgggatt gcctgctggg catcagtgag tgcgggcaac gtatggagaa catcgcccat
    23521 cgacgatgtt ttaacgatca aaacccgcat ccgtcaggct tcctcttgta acaatagcgt
    23581 gttgagttct tccagtacgc gctggggagt aatgtcgatc aagctctggt gataaccctc
    23641 cgcagcgtca cctttacgca ctttgtgata gccggtaatc agacggatca cgcgcgcttt
    23701 atgggatagc ggcggtgtga agtccgggct acttggacca tacagggcaa ccagcggacg
    23761 attgagcgcc gccgcaacgt gcatcaggcc agaatcgtta gtgacaatgg ctttacaggc
    23821 tgcaatcagg ataaccgctt gatcaagctg tgtttccccc gccaggttcc gacaccatgc
    23881 ctgctgctcg gtattcaaag cggcaagaat ctcattgccc gcttcatgat ctttcgcaga
    23941 gccaaacaga accacctgat aaccttcatc aatcagctgc tttgccagtt ccgcatagtg
    24001 gtagtgtggc cagcgttttg ccggaccaaa ctccgcaccc gggcaaaagc caatcatcgg
    24061 acgttctgat gaaagcgaaa attgattaca ggtatatgat ttttcacctt cgctcacctg
    24121 caactgcggc cataacaatg gctgcggcag atcttgtgcg gtacgcataa tgcctttgtc
    24181 ataggccagc gcgacatagc gttccaccat tagcggccag gcttctttat cgagcacgcg
    24241 tacatcgttg agtaaaccgt agcgcatctc gccgcgccag ccggtgcgat gaggaatacc
    24301 cgcgaagaaa ggcactaatg cagatttgaa ggagttgggt aagacgtagg cgcggtcgta
    24361 gcgcttttca cgcaggctat gacccagttt gcggcgttcg ccgatttcca gcgctccgtg
    24421 accgagaggc atagggatag cttcgttaac ttccggcatc cgcgataata atggacggca
    24481 ccatgccggt gccatcacat cgattatcgc ctggggatag cgcgcctgga gcgtgcgata
    24541 gagactttgc gacatcatca tgtcgccaac ccaagacggg ccgatcacca gtattttcat
    24601 gcagagctct tatgcgtcgc gattcagcca ggccatgtat tccgttacac cttcagcaac
    24661 ggttttgaac ggtttgtcgt aacccgccgc gcgcagattt gtcagatctg cctgagtgaa
    24721 cgcctggtag cggcctttca gcttatccgg gaacggaatg tattcgatct ggcctttctt
    24781 gtgataagcc agagtagcat ctgctacggc ctggaaggat tccgcacgac cagtaccgag
    24841 gttgaagata ccggaaacgc cattttccag gaaccacagg tttacatctg ccacgtcgcc
    24901 cacatagacg aagtcacgtt tgaagttctc gctaccttca aacagcttcg gtgattcacc
    24961 gttgttaagc tgagtgttga gatggaaagc gacgctcgcc atgctgcctt tatggccttc
    25021 acgcggtcca taaacgttga aatagcggaa gccaacaatc tgcgagttcg cttcaggcag
    25081 gatttggcga acatattcat caaacaggaa ttttgagtaa ccgtagacgt tcaacggttt
    25141 ttcgtactcg cgggattcaa taaagtcgga ggtgcgtccg ccgtaggtgg ctgcggaaga
    25201 agcgtacagg aacgggattt cgcgctccag gcagtagtgc agcagctcct tggagtattg
    25261 atagttgtta tccatcatat acttgccgtc ccactcggtg gtggaagagc atgcgccttc
    25321 gtggaaaatc gcttcgacat cgccgaactc ttcgccagcc ataatctgga tcaggaagtc
    25381 ttccttatcc atatagtccg cgatatccag atccaccagg ttcacaaact tggtgccgtc
    25441 tttcaggttg tccaccacca gaatatcggt gatgccttta tcattcaggg ccttaacgat
    25501 gttgctgccg ataaagcccg cgccgccggt aacgatgatc ataactgtaa ccttcgaatt
    25561 atggagtcag agacaatctc agacacgaat acttctatca tatcactata cggcgttggc
    25621 ttcagccatt cgccaaaagt gagattgatg gacgttattt atcctgaatt ttgcagaagt
    25681 gttaacgcgt catctcgtcg cgacgtataa gtttgggtaa tatgtgctgg aatttgccct
    25741 gtctggagaa tcgcaatgcg tggagaattt tatcagcagt taaccaacga tctggaaacc
    25801 gcacgggcgg aagggttgtt taaagaagag cgcattatta cgtctgcgca gcaagcagat
    25861 atcactgtgg ctgatggaag ccacgtcatt aacttttgtg ccaacaacta tctcgggctg
    25921 gcgaatcatc cggatctgat tgcggcagca aaggcgggaa tggattctca cggtttcggc
    25981 atggcttcgg tgcgttttat ttgcggcact caggacagcc ataaagagct tgaacaaaaa
    26041 ctggcggcat tcctggggat ggaagatgcg attctctact cttcctgctt tgatgctaac
    26101 ggtggactgt ttgaaacgct gctgggtgcg gaagacgcca ttatctccga cgcactgaac
    26161 cacgcgtcta ttattgatgg tgtgcgtctg tgcaaagcta aacgctatcg ctatgccaac
    26221 aacgatatgc aggagctgga agcacgtctg aaagaagcgc gtgaagccgg tgcgcgtcat
    26281 gtgctgattg ccaccgatgg tgtgttctca atggacggcg tgattgccaa cctgaaaggc
    26341 gtttgcgatc tggcagataa atacgatgcc ttggtgatgg tggatgactc ccacgcggtc
    26401 ggttttgtcg gtgaaaatgg tcgtggttcc catgaatact gcgatgtgat gggccgggtc
    26461 gatattatca ccggtacgct tggtaaagcg ctgggcgggg cttctggtgg ttataccgcg
    26521 gcgcgcaaag aggtggttga gtggctgcgc cagcgttctc gtccgtacct gttctccaac
    26581 tcgctggcac cggccattgt tgccgcgtcc atcaaagtac tggagatggt cgaagcgggc
    26641 agcgaactgc gtgaccgtct gtgggcgaac gcgcgtcagt tccgtgagca aatgtcggcg
    26701 gcgggcttta ccctggcggg agccgatcac gccattattc cggtcatgct tggtgatgcg
    26761 gtagtggcgc agaaatttgc ccgagagctg caaaaagagg gcatttacgt caccggtttc
    26821 ttctatccgg tcgttccgaa aggtcaggcg cgtattcgta cccagatgtc tgcggcgcat
    26881 acccctgagc aaattacgcg tgcagtagaa gcattcacgc gtattggtaa acaactgggc
    26941 gttatcgcct gaggatgtga gatgaaagcg ttatccaaac tgaaagcgga agagggcatc
    27001 tggatgaccg acgtccctgt accggaactc gggcataacg atctgctgat taaaatccgt
    27061 aaaacagcca tctgcgggac tgacgttcac atctataact gggatgagtg gtcgcaaaaa
    27121 accatcccgg tgccgatggt cgtaggccat gaatatgtcg gtgaagtggt aggtattggt
    27181 caggaagtga aaggcttcaa aatcggcgat cgcgtttctg gcgaaggcca tatcacctgc
    27241 ggtcactgtc gcaactgtcg tggcggtcgt acccatctgt gccgcaacac gatcggcgtc
    27301 ggcgttaacc gtccgggctg cttcgccgaa tatctggtga tcccggcgtt caacgccttc
    27361 aaaatcccag acaatatctc cgacgacctg gcttccattt ttgatccctt cggtaacgcc
    27421 gtgcataccg cgctgtcgtt cgatctggtt ggcgaggatg tgctggtctc tggtgcaggt
    27481 ccgataggta ttatggctgc ggcggtggcg aaacacgttg gtgcacgcaa tgtggtgatc
    27541 actgatgtta acgaataccg ccttgagctg gcgcgcaaaa tgggtatcac ccgtgcggtt
    27601 aacgtcgcga aagaaaacct taatgatgtg atggctgaac tgggcatgac cgaaggcttt
    27661 gatgtcggtc tggaaatgtc cggtgcgccg ccagcgtttc gtaccatgct tgacaccatg
    27721 aaccacggcg gccgtattgc gatgctgggt attccgccgt ctgatatgtc tatcgactgg
    27781 accaaagtga tctttaaagg cttgttcatt aaaggtattt acggtcgtga gatgtttgaa
    27841 acctggtaca agatggcggc gctgattcag tctggcctcg atctctcgcc gatcattacc
    27901 catcgtttct ctatcgatga tttccagaag ggctttgacg ctatgcgttc gggccagtcc
    27961 gggaaagtaa ttctgagctg ggattaacac gaacaagggc tggtattcca gcccttttat
    28021 ctggggataa tctgttgaat atgtaaaatc ctgtcagtgt aataaagagt tcgtaattgc
    28081 gctgatctct tatatagctg ttctcattat ctctctaccc tgaagtgact ctctcacctg
    28141 taaaaataat atctcacagg cttaatagtt tcttaataca aagcctgtaa aacgtcagga
    28201 taacttcaga ggtcgtcggt aatttatgat gaacagcacc aataaactta gtgttattat
    28261 tccgttatat aatgcgggcg atgatttccg cacttgtatg gaatctttaa ttacgcaaac
    28321 ctagactgct ctggaaatca ttattattaa cgatggttca acggataatt ctgttgaaat
    28381 agcaaagcat tacgcagaaa actatccgca cgttcgtttg ttgcatcagg cgaatgctgg
    28441 cgcatcggta gcgcgtaatc gtgggatcga ggtggcgacg ggcaaatatg tcgcttttgt
    28501 cgatgctgac gatgaggtct atcccaccat gtacgaaacg ctgatgacca tggcgttaga
    28561 ggacgacctc gacgtggcgc agtgcaacgc tgactggtct tttcgtgaaa cgggagaaac
    28621 ctggcaatcc atccccagcg atcgccttcg ctcaaccggc gtattaaccg gcccggactg
    28681 gctgcggatg gggctttctt cgcgccgttg gactcacgtt gtctggatgg gggtttatcg
    28741 ccgtgatgtt attgttaaaa ataacattaa atttattgcc ggattacatc atcaggatat
    28801 tgtctggacg acagaattca tgtttaacgc gctgcgtgcg cgatataccg agcaatcatt
    28861 atataaatat tatctgcata atacgtcagt gagtcggttg catagacaag ggaataaaaa
    28921 ccttaattat caacgtcact atattaagat tacccgcctg ctggagaaat taaatcgaaa
    28981 ttatgccgac aaaattacga tttatccgga atttcatcag caaataactt acgaagcatt
    29041 gcgtgtttgc catgcggtgc gcaaagagcc ggatattctt acccgccaac ggatgattgc
    29101 cgagatattt acttccggta tgtataagcg cctgattacc aatgtgcgca gcgtgaaggt
    29161 cggttaccag gcgttactgt ggtctttccg cttatggcaa tggcgcgaca aaacgcggtc
    29221 gcaccatcgc attacgcgta gcgcctttaa tttgcgctag cgttaaactt cgccgcttcg
    29281 ggctgtttgc cccagccttg ccactgatgc tggaagtaaa cgatcagcgt gctctggctg
    29341 atgctttcgc ttaatacttc aaagaagcga ttagcataaa caggttctat cggtttcttc
    29401 ggtttgcaca gcttcacgcc acggaacgga ttacgcggtg catcaggcac agcgtttttc
    29461 ggcggtgtag aagtatcaac ctgcggttca ttcagcaagc tgctggcttt caccagcgta
    29521 atgtctggcg gcaggttata aaccatctgt tgtagcacgc gtaccgttaa agggtgagga
    29581 tgcccaatgg caatggttga accgttgcga cgcgccagat caattgcgcg attaaattgc
    29641 acacggatgt ccgcttcgtt ctgcgaatcg tcgaggaaca ccttccgttt aataaccttc
    29701 acgccagtac cttgcgcggc gcgcatcgcc tgggtattac cgatggttac gctgtcgagg
    29761 aagtaaagat tgtaacgctc cagtgcctgc atcactttct gcataccaaa caggttagag
    29821 gtcatcttgc tacccatgtg gttgttgatc cccacggcat agggcacgtt attgaccgca
    29881 ctacgaataa tgcgctcaat ttcgtcgctg ctcatctccg ggcgcagcgt atttttctcc
    29941 agcggctgtt tactcagtgg tgccatcggg agatgaatca acacttcgtg cccgctgtta
    30001 tgcgctttgg tcgccatctc tctggcgtgc ggtgaatcgg gtaatacagc gacggagata
    30061 gcggagggca tcgccagcac ctggttttcg ttgtgcgggc gatacccaaa atcatcaatg
    30121 acgatggcaa gtttgccagc aagtacgggg gaggagagcg ccaacagagc ggcaaatgca
    30181 agaacgttac gacgaaatgg aaacaaaact tatcttccca accacggctg tggattgacc
    30241 gcctgaccct ggcggcgaat ttcgaaatag agtgaaggcc gaccctgacc gccactgctg
    30301 cccaccagtg caattggctg gcccgcgcga acctgcgaac caacgctcac cagtgcgctc
    30361 tgattatagc cgtaaagact catgtcgcct ttaccgtgct caaccaccac caccagaccg
    30421 taaccttgca gccagtcagc cagaatcact cgaccgtcgg caattgcttt aacttcagta
    30481 ccttcagagg caccgataac catccctttc cagcgtaatt caccctgtag ctgctcgcca
    30541 tagcgatgca gcgtcggccc gcgaaccggc cagaatgcct gaccacgcgg cgcacccaga
    30601 ccaccagttc gggacatcag cgatttttcg ctttcggtcg gtttgtaggt ggtgcctttg
    30661 cgcgtcgctt ctttctggcg gtcgcgaacc gcctgggcct cacgcgcttc tcgttcagca
    30721 cgcgctttcg ccgcggcttc cgcacgggca atgctgttac gcagacggga ttcgttggca
    30781 cgcagttcac ttagctgctg ctgaccttgc tggatggaag attccagccc cgccagcgtc
    30841 tttttacgct cgttcagagc ctgagtcagt ttcgcctgtt gggcgcgctg ttcatataac
    30901 agcgtttgtt gctcgctttg tttctcttcc agttcagcac gctgcatggc gacttcttca
    30961 cgcgtttgct tcaactgggc aatggtttct tgtcgcgcct ggttgagata gccgaaataa
    31021 gcctgtaaac gctgtccaca ctggctttct tcaccgctga gaattagctg aataccggta
    31081 tgctcgccct gacggaatgc ggcatccagt tgtgcggcga ggctgcgctc ctgggcggct
    31141 ttttgctgct ccagtttggc aatcgacgcg ttcatctcat caatctgttt attgagttga
    31201 ttgagcgtgt tttgcgtttc gcgcagctta cgggtggctt cagagatcgc ttcttcctgc
    31261 tttttcaatt gtgcgagcag gcttgcgcgt tgttgttgct tttggcgtac cgcgcgctct
    31321 tttgcggcga tatcggcctg aatagatttg agttggtcac gctcatccgc gtgggcggaa
    31381 aaggcgcaca acaatacgcc agcgctaaga acgctggcgt agatgatggg cctgattgca
    31441 aacctgcgcg gtttcacggc ccgtgtcatg gtattaatcg cctttcccct catggggagg
    31501 gattattcca cgatgaacag cggcttacca gtcatctctt gcgggatttc catacccatc
    31561 agcgacaaca tggtcggcgc gatgtcagaa agtttgccgc ctgcaaccgc tttcacgttc
    31621 ttatcaccaa cgtaaatcag cggaactggc aggttggtgt gtgccgtgtg tgcctgaccc
    31681 gttgccggat cgcgcatctg ttcagcgtta ccgtggtcag cggtgatcag cagttgtcca
    31741 cccacggact caaccgcttt cgcgacttct tccacgcagt gatccagtgc ttcaaccgct
    31801 ttaaccgccg cttccatcac cccggtgtga cctaccatgt cgccgttcgg atagttacag
    31861 ataatggtgt cgtatttgcc gctcttgatg gccgcaacca gtttttcggt cagctctgcg
    31921 gagctcattt ccggttgcag atcgtaggta gccactttcg gtgagttgat cagaatgcga
    31981 tcttcgcctt tgaatgactc ttctacgcca ccgttgaaga agaaagtaac gtgggcatat
    32041 ttttcggttt cggaaatacg caactgagtt ttgtcgtttt tcgccatcca ctcgccgaag
    32101 gtgttaacca gggaagcggg tgggtaagca accgcagttt tgatgtcagc ggcgtattcg
    32161 gtcagcatca cgaaatcgac gttaaccact ttcttacgcg cgaagccgtc gaaatctgcg
    32221 tttacgaatg cacgagtgat ttcgcgcgcg cggtcagcac ggaagttcat gaaaatcagc
    32281 gcatcgccgt cttccattgc cgcatctggc tgaccttctg cacgaataac ggtcgctttc
    32341 acgaactcgt cgttttcgtc gcgagcataa gctgcctgca aaccagcaac ggcagtatcg
    32401 gcctggaact caccctgcgc cagagtcagc aggtcatagg ctttttcaac gcgatcccaa
    32461 cggttatcgc ggtccatcgc gtagtaacga ccaatgatgg acgctacgcg gcctttaccc
    32521 agcgcagcaa atttctcttc aaattttttc agcgaggatt cagcactgcg cggcggggtg
    32581 tcgcgaccgt caaggaatgc gtgcaggtag attttttctg cgccgcgttc agctgccagt
    32641 tctaccatcg ccatgatgtg atcttcgtgg ctgtgtacgc cgcctgcgga gagcagaccc
    32701 ataatgtgta ctgctttacc tgcgtttttc gctttatcta ccgcgccagt cagcactgga
    32761 ttagcaaaga aagcgcgatc tttgatttca acgtccagac gagtcaggtc ctgatacacg
    32821 atgcggccgg cacccaggtt aacgtggcct acttcggagt tacccatctg acggtcaggc
    32881 agaccgactt ccagaccgga agcgtcgatc agggtatgcg gacgattggc ccacagtgca
    32941 tccattaccg gggttttagc actaaaaatg gcgttatcct gctgttcttc acgatagcca
    33001 tagccatcca gaatcaccag taccataggt tttttagaaa ccgacattgc gacaacctca
    33061 tactcaagag tcaaaatttg cgtaatttta ctacagctga atcgataaaa tagcctctga
    33121 agatcaaaat atacgaccgc tcgcggcaag ccgatgccag atttgcgctt ttttttcgtc
    33181 gtgccccgca aaattgcatt ccagttaacg cgctggctgt atttgccgca ccgcgcaggt
    33241 atactccttt cctggttttt ttaatcacta cgtcgggagt tgttaccccc catgcaagaa
    33301 attatgcaat ttgttggccg tcatcccata ctgagtatcg cttggatcgc gttactggtg
    33361 gcggttcttg tgactacgtt taagagcctg acctcgaaag tgaaggtgat tactcgtggt
    33421 gaagctacgc gtctgatcaa caaagaagac gctgtggttg tggatttacg tcagcgtgat
    33481 gacttccgta aaggccatat cgcaggttct atcaatctgt tgccgagcga aatcaaagcc
    33541 aacaatgttg gtgagcttga gaagcacaaa gacaaaccgg ttatcgtggt agacggttct
    33601 ggcatgcagt gccaggagcc tgcaaacgcg ctgacgaaag ctggttttgc gcaagtattc
    33661 gtgctgaaag agggcgttgc tggctgggct ggcgaaaact tgcctctggt gcgcggcaaa
    33721 taatttacct gtcgtctttc atgatgcagg ccgttagctg caattaggcc cccagcgact
    33781 tattgttata agcttctggg gacactccat tttgcagccg ccctgcaaca tgaaatttca
    33841 gggtaaaaaa acaggagcta aattatggcc aatgttgaaa tctataccaa agaaacctgc
    33901 ccgtattgcc atcgtgcaaa agcactgctg agcagcaagg gcgtgagttt tcaggagttg
    33961 ccgatcgatg gcaatgccgc caagcgtgaa gagatgatca aacgcagcgg tcgcaccacg
    34021 gttccccaga tttttattga cgcacagcac attggcggct gtgatgactt gtatgcattg
    34081 gatgcacgtg gtggactgga tcccctgctg aaataacgtg tgaacgttgg cattacattg
    34141 cgcagtattt aaggacaaca cttaagggtt ttctacacat gtcagaacaa aacaacactg
    34201 aaatgacttt ccagatccaa cgtatttata ccaaggatat ctctttcgaa gcgccgaacg
    34261 cgccgcacgt tttccagaaa gattggcaac cagaagttaa acttgatctg gatacggcat
    34321 cttcccaact ggcagatgac gtatacgaag tggtactgcg tgttaccgta acggcctccc
    34381 tgggcgaaga aaccgcgttc ctgtgtgaag ttcagcaggg cggtattttc tccatcgcgg
    34441 gtatcgaagg cacccagatg gcacattgcc tgggcgcata ctgcccgaac attctgttcc
    34501 cgtatgctcg tgagtgcatc accagcatgg tatcccgcgg tacattcccg caactgaacc
    34561 ttgcgccggt taacttcgat gcgctgttca tgaactattt gcagcagcag gctggcgaag
    34621 gtactgaaga acatcaggat gcctgatgaa ccaacgtaat gcttcaatga ctgtgatcgg
    34681 tgccggctcg tacggcaccg ctcttgccat taccctggca agaaatggcc acgaggttgt
    34741 cctctggggc catgaccctg aacatatcgc aacgcttgaa cgcgaccgct gtaacgccgc
    34801 gtttctcccc gatgtgcctt ttcccgatac gctccatctt gaaagcgatc tcgccactgc
    34861 gctggcagcc agccgtaata ttctcgtcgt cgtacccagc catgtctttg gtgaagtgct
    34921 gcgccagatt aaaccgctga tgcgtcctga tgcgcgtctg gtgtgggcga ccaaagggct
    34981 ggaagcggaa accggacgtc tgttacagga cgtggcgcgt gaggccttag gcgatcaaat
    35041 tccgctggcg gttatctctg gcccaacgtt tgcgaaagaa ctggcggcag gtttaccgac
    35101 ggctatttcg ttggcctcta ccgaccagac ctttgccgat gatctccaac aattgctgca
    35161 ctgtggcaaa agtttccgcg tttacagcaa cccggatttc atcggtgtgc agcttggcgg
    35221 tgcggtgaaa aacgtcattg ccattggcgc ggggatgtcc gacggtatcg gttttggtgc
    35281 gaatgcgcgt acggcactga tcacccgtgg gctggctgaa atgtcgcgtc ttggcgcggc
    35341 gctgggtgcc gagcctgcca cctttatggg catggcgggg ctgggcgatc ttgtgcttac
    35401 ctgtaccgac aaccagtcgc gtaaccgccg ttttggcatg atgctcggtc agggcatgga
    35461 tgtacaaagc gcgcaggaga agattggtca ggtggtggaa ggctaccgca atacgaaaga
    35521 agtccgcgaa ctggcgcatc gcttcggcgt agaaatgcca ataaccgagg aaatttatca
    35581 agtattatat tgcggaaaaa acgcgcgcga ggcagcattg acgttattag gtcgtgcacg
    35641 caaggacgag cgcagcagcc actaacccca gggaaccttt gttaccgcta tgacccggcc
    35701 cgcgcagaac gggccggtca ttatctcatc gtgtggagta agtaatgtcg tgtgaagaac
    35761 tggaaattgt ctggaacaat attaaagccg aagccagaac gctggcggac tgtgagccaa
    35821 tgctggccag tttttaccac gcgacgctac tcaagcacga aaaccttggt agtgcactga
    35881 gctacatgct ggcgaacaag ctgtcatcgc caattatgcc tgctattgct atccgtgaag
    35941 tggtggaaga agcctacgcc gctgacccgg aaatgatcgc ctctgcggcc tgtgatattc
    36001 aggcggtgcg tacccgcgac ccggcagtcg ataaatactc aaccccgttg ttatacctga
    36061 agggttttca tgccttgcag gcctatcgca tcggtcactg gttgtggaat caggggcgtc
    36121 gcgcattggc aatctttctg caaaaccagg tttctgtgac gttccaggtc gatattcacc
    36181 cggcagcaaa aattggccgt ggcattatgc tcgaccacgc cacgggcatc gttgtgggtg
    36241 aaacggcggt aattgaaaac gacgtatcga ttctgcaatc tgtgacgctt ggcggtacgg
    36301 gtaaatctgg tggtgaccgt cacccgaaaa ttcgtgaagg tgtgatgatt ggcgcgggcg
    36361 cgaaaatcct cggcaatatt gaagttggtc gcggcgcgaa gattggcgca ggttccgtgg
    36421 tgctgcaacc ggtgccgccg catacaaccg ccgctggcgt tccggctcgt attgtcggta
    36481 aaccagacag cgataagcca tcaatggata tggaccagca tttcaacggt attaaccata
    36541 catttgagta tggggatggg atctaatgtc ctgtgatcgt gccggatgcg atgtaatcat
    36601 ctatccggca tacagtaact aatctctcaa caacgctccc ggatatccca gctgccgcca
    36661 ggcttcatac accactaccg acaccgcatt ggacagattc atgctgcggc tgtccggcac
    36721 catcggaatg cgaatttttt gttcagcggg cagggcatca agaatgctcg ctggcaggcc
    36781 gcgtgtttcc gggccgaaca tcagataatc gccatcctga tagcttacgg cgctgtgagc
    36841 aggcgtacct ttcgtggtga gggcaaacag gcgctgggga ttttctgctt cgaggaacgc
    36901 gcgatagtca tgatgacgcg taacggcggt aaactcgtga tagtccagcc ccgcgcggcg
    36961 caggcgctta tcgtcccagg caaatcccat cggttcgatg atatgcagac gaaagccggt
    37021 attagcgcaa agacggatga tgttgccagt atttggcgga atttctggtt cgtaaagtac
    37081 gatgtttagc atactgcccc cttagtgcgg gggcaggata gcagaaaatt ggtgaaccgt
    37141 gcggcctttt cccctcaccc cggccctccc cctgtctctt gatcaggaga tctgatcaag
    37201 agacagctcc ctgagggaga gggttagggg ggtgccccta ttaagttgtc aagcatgtta
    37261 tgacccctgc ggggtataaa aagtcccgtc gcgcatcatg gcgaacagaa
//
LOCUS       DAJTMC010000019        36824 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.019, whole genome shotgun sequence.
ACCESSION   DAJTMC010000019 DAJTMC010000000
VERSION     DAJTMC010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36824
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.019"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            582..668
                     /gene="leuL"
                     /locus_tag="OO130_000858"
     CDS             582..668
                     /gene="leuL"
                     /locus_tag="OO130_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HCR5364366.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            761..2332
                     /gene="leuA"
                     /locus_tag="OO130_000859"
     CDS             761..2332
                     /gene="leuA"
                     /locus_tag="OO130_000859"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HCR5364367.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            2332..3423
                     /gene="leuB"
                     /locus_tag="OO130_000860"
     CDS             2332..3423
                     /gene="leuB"
                     /locus_tag="OO130_000860"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HCR5364368.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLDAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDITGKNIANPIAQILSLALLLRYSLDADDAACAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            3426..4826
                     /gene="leuC"
                     /locus_tag="OO130_000861"
     CDS             3426..4826
                     /gene="leuC"
                     /locus_tag="OO130_000861"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HCR5364369.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGQVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            4837..5442
                     /gene="leuD"
                     /locus_tag="OO130_000862"
     CDS             4837..5442
                     /gene="leuD"
                     /locus_tag="OO130_000862"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HCR5364370.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPVIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIAAYEAKQPAFMN"
     gene            complement(5491..6573)
                     /locus_tag="OO130_000863"
     CDS             complement(5491..6573)
                     /locus_tag="OO130_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5364371.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNTIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNAPLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYEDNSAREVVMFSIAAGIFTLSLVLIAFML
                     PSVARVELPSENALSMQGGWQDSNVRMLFVASTLMWTCNTMYIIDMPLWISSELGLPD
                     KLAGFLMGTAAGLEIPAMILAGYYVKRYGKRRMMVIAVAAGVLFYTGLIFFHSRMALM
                     TLQLFNAVFIGIVAGIGMLWFQDLMPGRAGAATTLFTNSISTGVILAGVIQGAIAQSW
                     GHFAVYWVIAVISVVALFLTAKVKDV"
     gene            complement(6674..6805)
                     /gene="sgrT"
                     /locus_tag="OO130_000864"
     CDS             complement(6674..6805)
                     /gene="sgrT"
                     /locus_tag="OO130_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HCR5364372.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            6894..8549
                     /gene="sgrR"
                     /locus_tag="OO130_000865"
     CDS             6894..8549
                     /gene="sgrR"
                     /locus_tag="OO130_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HCR5364373.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSTLRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     TTNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVNYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      8583..8682
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            8713..9696
                     /gene="thiB"
                     /locus_tag="OO130_000866"
     CDS             8713..9696
                     /gene="thiB"
                     /locus_tag="OO130_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HCR5364374.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISGWQRAVSR"
     gene            9672..11282
                     /gene="thiP"
                     /locus_tag="OO130_000867"
     CDS             9672..11282
                     /gene="thiP"
                     /locus_tag="OO130_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HCR5364375.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPDEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALIQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLQQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            11266..11964
                     /gene="thiQ"
                     /locus_tag="OO130_000868"
     CDS             11266..11964
                     /gene="thiQ"
                     /locus_tag="OO130_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HCR5364376.1"
                     /translation="MLKLTDITWLYQHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTEELL
                     SGKASASAILGITG"
     gene            complement(12077..12841)
                     /locus_tag="OO130_000869"
     CDS             complement(12077..12841)
                     /locus_tag="OO130_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5364377.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(12927..13805)
                     /gene="araC"
                     /locus_tag="OO130_000870"
     CDS             complement(12927..13805)
                     /gene="araC"
                     /locus_tag="OO130_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HCR5364378.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            14144..15844
                     /gene="araB"
                     /locus_tag="OO130_000871"
     CDS             14144..15844
                     /gene="araB"
                     /locus_tag="OO130_000871"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HCR5364379.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQTQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVPTL
                     "
     gene            15855..17357
                     /gene="araA"
                     /locus_tag="OO130_000872"
     CDS             15855..17357
                     /gene="araA"
                     /locus_tag="OO130_000872"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HCR5364380.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQHLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            17557..18252
                     /gene="araD"
                     /locus_tag="OO130_000873"
     CDS             17557..18252
                     /gene="araD"
                     /locus_tag="OO130_000873"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HCR5364381.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQSGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMEIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            18327..20678
                     /gene="polB"
                     /locus_tag="OO130_000874"
     CDS             18327..20678
                     /gene="polB"
                     /locus_tag="OO130_000874"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HCR5364382.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGSENGDASSLDFELEYVASRPQLLEKLNAWFANYDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRETIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            20842..23748
                     /gene="rapA"
                     /locus_tag="OO130_000875"
     CDS             20842..23748
                     /gene="rapA"
                     /locus_tag="OO130_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HCR5364383.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHMPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLLDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            23760..24419
                     /gene="rluA"
                     /locus_tag="OO130_000876"
     CDS             23760..24419
                     /gene="rluA"
                     /locus_tag="OO130_000876"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HCR5364384.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(24536..25351)
                     /gene="djlA"
                     /locus_tag="OO130_000877"
     CDS             complement(24536..25351)
                     /gene="djlA"
                     /locus_tag="OO130_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HCR5364385.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            25606..27960
                     /gene="lptD"
                     /locus_tag="OO130_000878"
     CDS             25606..27960
                     /gene="lptD"
                     /locus_tag="OO130_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HCR5364386.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWQHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVEHFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            28013..29299
                     /gene="surA"
                     /locus_tag="OO130_000879"
     CDS             28013..29299
                     /gene="surA"
                     /locus_tag="OO130_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HCR5364387.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            29299..30288
                     /gene="pdxA"
                     /locus_tag="OO130_000880"
     CDS             29299..30288
                     /gene="pdxA"
                     /locus_tag="OO130_000880"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HCR5364388.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTAGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMILNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            30285..31106
                     /gene="rsmA"
                     /locus_tag="OO130_000881"
     CDS             30285..31106
                     /gene="rsmA"
                     /locus_tag="OO130_000881"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HCR5364389.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            31109..31486
                     /gene="apaG"
                     /locus_tag="OO130_000882"
     CDS             31109..31486
                     /gene="apaG"
                     /locus_tag="OO130_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HCR5364390.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            31493..32335
                     /gene="apaH"
                     /locus_tag="OO130_000883"
     CDS             31493..32335
                     /gene="apaH"
                     /locus_tag="OO130_000883"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HCR5364391.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGTLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(32413..32892)
                     /gene="folA"
                     /locus_tag="OO130_000884"
     CDS             complement(32413..32892)
                     /gene="folA"
                     /locus_tag="OO130_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HCR5364392.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(33084..34946)
                     /gene="kefC"
                     /locus_tag="OO130_000885"
     CDS             complement(33084..34946)
                     /gene="kefC"
                     /locus_tag="OO130_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HCR5364393.1"
                     /translation="MDSHTLIQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLATSSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLLENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(34939..35469)
                     /gene="kefF"
                     /locus_tag="OO130_000886"
     CDS             complement(34939..35469)
                     /gene="kefF"
                     /locus_tag="OO130_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HCR5364394.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGKALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(35577..36824)
                     /locus_tag="OO130_000887"
                     /pseudo
     CDS             complement(35577..36824)
                     /locus_tag="OO130_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT         8959 a        10084 c         9685 g         8096 t
ORIGIN      
        1 tgcatataaa ataagaaaaa gcaaaatgag taaaattcgg aaaacataaa gatacgggtt
       61 ctgataaaaa cagaatccat tgccgtggaa tgagtcattt accacagcat aataatccat
      121 aatgtttttg ttgcgaaaac aatctaatca taactaccgc gaatactcaa tcatctacaa
      181 aatggattaa atgtgcttat ttaataatta atttatatat ttaatgcatt aattattaac
      241 attaatggat caataatatt caccatatca atatcaaaaa aaattcgcaa aacatataat
      301 tcaatacaaa tcatcaggat aggttttgca acgcgtgcat tttgtcccct ttttcctcgt
      361 tgattagatg caaaaattta ttctgaaata tgtcaaccta tgaaaagtgt cggtcgttaa
      421 acagaaatta atatcgcttg ctttaaccac cgcagcacaa ttagctaatt ttacggatgc
      481 agaactcgcg ctggcgggac gtttttattg cgtcagggtt gacatccgtt tttgtatcca
      541 gtaactctaa aagcatatcg cattcatctg gagctgattt aatgactcac atcgttcgct
      601 ttatcggtct actactacta aacgcatctt ctttgcgcgg tagacgagtg agcggcatcc
      661 agcattaagc cagcacgcag tcaaacaaaa aacccgcgcc attgcgcggg tttttttatg
      721 cccgaagcga ggcgctctaa aagagacaag gacccaaacc atgagccagc aagtcattat
      781 tttcgatacc acattgcgcg acggtgaaca ggcgttacag gcaagcttga gtgtgaaaga
      841 aaaactgcaa attgcgctgg cccttgagcg tatgggtgtt gacgtgatgg aagtcggttt
      901 ccccgtctct tcgccgggcg attttgaatc agtgcaaacc atcgcccgcc aggttaaaaa
      961 cagccgcgta tgcgcgttag ctcgctgcgt ggagaaagat atcgacgtgg cggccgaatc
     1021 cctgaaagtc gccgaagcct tccgtattca tacctttatt gccacttcgc caatgcacat
     1081 cgccaccaag ctgcgcagca cgctggatga agtgatcgaa cgcgctatct atatggtgaa
     1141 acgcgcccgt aattacaccg atgatgttga attttcttgc gaagatgccg ggcgtacccc
     1201 cattgccgat ctggcgcgag tggtcgaagc ggcgattaat gccggtgcca ccaccatcaa
     1261 cattccggac accgtgggct acaccatgcc gtttgagttc gccggaatca tcagcggtct
     1321 gtatgaacgc gtacctaaca tcgacaaagc cattatttcc gtacataccc acgacgattt
     1381 aggcctggcg gtcggcaact cactggcggc ggtacatgcc ggtgcacgcc aggtggaagg
     1441 tgcaatgaac gggatcggcg agcgtgccgg taactgttcg ctggaagaag tcatcatggc
     1501 gattaaagtt cgtaaggata ttctcaacgt ccacaccgcc attaatcacc aggagatatg
     1561 gcgcaccagc cagttagtta gccagatttg taatatgccg atcccggcaa acaaagccat
     1621 tgttggcagc ggcgcattcg cccactcctc cggtatccac caggatggcg tgctgaaaaa
     1681 ccgcgaaaac tacgaaatca tgacaccaga atctattggt ctgaaccaaa tccagctgaa
     1741 tctgacctct cgttcggggc gtgcggcggt gaaacatcgc atggatgaga tggggtataa
     1801 agaaagtgaa tataatttag acaatttgta cgatgctttc ctgaagctgg cggacaaaaa
     1861 aggtcaggtg tttgattacg atctggaggc gctggccttc atcggtaagc agcaagaaga
     1921 gccggagcat ttccgtctgg attacttcag cgtgcagtct ggctctaacg atatcgccac
     1981 cgccgccgtc aaactggcct gtggcgaaga agtcaaagca gaagccgcca acggtaacgg
     2041 tccggtcgat gccgtctatc aggcaattaa ccgcatcact gactataacg tcgaactggt
     2101 gaaatacagc ctgaccgcca aaggtcacgg taaagatgcg ctgggtcagg tggatattgt
     2161 cgccaactac aacggtcgcc gcttccacgg cgtcggcctg gccaccgata ttgtcgagtc
     2221 ctccgccaaa gccatggtgc acgtactgaa caatatctgg cgtgccgcag aagtcgaaaa
     2281 agagttgcaa cgcaaagctc aacacaacga aaacaacaag gaaaccgtgt gatgtcgaag
     2341 aattaccata ttgccgtatt gccgggggac ggtattggtc cggaagtgat gacccaggcg
     2401 ctgaaagtgc tggatgccgt gcgcaaccgc tttgcgatgc gcatcaccac cagccattac
     2461 gatgtaggcg gcgcagccat tgataaccac gggcaaccac tgccgcctgc gacggttgaa
     2521 ggttgtgagc aagccgatgc cgtgctgttt ggctcggtag gcggtccgaa atgggaacat
     2581 ttaccaccag accagcaacc agaacgcggc gcgctgttgc ctttgcgtaa gcacttcaaa
     2641 ttattcagca acctgcgtcc ggcaaaactg tatcaggggc tggaagcatt ctgtccgctg
     2701 cgtgctgaca ttgccgctaa cggcttcgac atcctgtgcg tgcgcgaact gaccggcggc
     2761 atctatttcg gtcagccaaa aggccgcgaa ggtagcggac agtatgaaaa agcgtttgat
     2821 accgaggtgt atcaccgttt tgagatcgaa cgtatcgccc gcatcgcgtt tgaatctgcc
     2881 cgcaagcgtc gccacaaagt cacctcaatc gacaaagcca acgtgctgca atcctctatt
     2941 ttatggcggg agatcgttaa cgagatcgcc acggaatacc cggatgtcga actggcgcat
     3001 atgtacatcg acaacgccac catgcagctg attaaagatc catcacagtt tgacgtcctg
     3061 ctgtgctcca acctgtttgg cgacattctg tctgacgagt gcgcaatgat cactggctcg
     3121 atggggatgt tgccttccgc cagcctgaac gagcaaggtt ttggtctgta tgaaccggca
     3181 ggcggctcag caccagatat cacaggcaaa aacatcgcca acccgattgc gcaaattctg
     3241 tcgctggcac tgctgctgcg ctacagcctg gatgccgatg atgcggcttg cgccattgaa
     3301 cgcgccatta accgcgcatt agaagaaggc attcgcaccg gggatttagc ccgtggcgct
     3361 gccgccgtta gtaccgatga aatgggcgat atcattgccc gctatgtggc agaaggggtg
     3421 taatcatggc taaaacgtta tacgaaaaat tattcgacgc tcacgtagtg tacgaagccg
     3481 aaaacgaaac cccgctgtta tatatcgacc gccacctggt gcatgaagtg acctcaccgc
     3541 aggcgttcga tggtctgcgc gcccacggtc gcccggtacg tcagccgggc aaaaccttcg
     3601 ccactatgga tcacaacgtc tctacccaga cgaaagacat taatgcctgt ggtgaaatgg
     3661 cgcgcatcca gatgcaggag ctgattaaaa actgcaaaga atttggcgtc gaactgtatg
     3721 acctgaatca cccgtatcag gggatcgtcc acgtaatggg gccggaacag ggcgtcacct
     3781 tgccggggat gaccattgtc tgcggcgact cgcataccgc cacccacggc gcgtttggcg
     3841 cactggcctt tggtatcggc acttccgaag ttgaacacgt actggcaacg caaaccctga
     3901 aacagggccg cgcaaaaacc atgaaaattg aagtccaggg caaagccgcg ccgggcatta
     3961 ccgcaaaaga tatcgtgctg gcaattatcg gtaaaaccgg tagcgcaggc ggcaccgggc
     4021 atgtggtgga gttttgcggc gaagcaatcc gtgatttaag catggaaggt cgtatgaccc
     4081 tgtgcaatat ggcaatcgaa atgggcgcaa aagccggtct ggttgcaccg gacgaaacca
     4141 cctttaacta tgtcaaaggc cgtttgcatg cgccgaaagg caaagatttc gacgacgccg
     4201 ttgcctactg gaaaaccctg caaaccgacg aaggcgcaac tttcgatacc gttgtcactc
     4261 tgcaagcaga agaaatttca ccgcaggtca cctggggcac caatccaggc caggtgattt
     4321 ccgtgaacga caatattccc gatccggctt cgtttgccga tccggttgaa cgcgcgtcgg
     4381 cagaaaaagc gctggcctat atggggctga aaccgggcat tccgctgacc gaagtggcta
     4441 tcgacaaagt gtttatcggt tcctgtacca actcgcgcat tgaagattta cgcgcggcag
     4501 cggagatcgc caaagggcga aaagtcgcgc caggcgtgca ggcactggtg gttcccggct
     4561 ctggccaggt aaaagcccag gcggaagcgg aaggtctgga taaaatcttt attgaagccg
     4621 gttttgaatg gcgcttgcct ggctgctcaa tgtgtctggc gatgaacaac gaccgtctga
     4681 atccgggcga acgttgtgca tccaccagca accgtaactt tgaaggccgc caggggcgcg
     4741 gcgggcgcac gcatctggtc agcccggcaa tggctgccgc tgctgctgtg accggacatt
     4801 tcgccgacat tcgcaacatt aaataaggag cagaccatgg cagagaaatt tatcaaacac
     4861 acaggcctgg tggttccgct ggatgccgcc aatgtcgata ccgatgcaat catcccgaaa
     4921 cagtttttgc agaaagtgac ccgtacgggt tttggcgcgc atctgtttaa cgactggcgt
     4981 tttctcgatg aaaaaggcca acagccaaac ccggacttcg tgctgaactt cccgcagtat
     5041 cagggcgctt ccattttgct ggcacgagaa aacttcggct gtggctcctc gcgtgagcac
     5101 gcgccctggg cattgaccga ctacggtttt aaagtggtga ttgcgccgag ttttgctgac
     5161 atcttctacg gcaatagctt taacaaccag ctgctgccgg tgaaattaag cgatgcagaa
     5221 gtggacgaac tgtttgcgct ggtgaaagcc aatccggtga tccatttcga cgtggatctg
     5281 gaagcgcaag aggtgaaagc gggagagaaa acctatcgct ttaccatcga tgccttccgc
     5341 cgccactgca tgatgaacgg tctggacagt attgggctta ccttgcagca cgacgacgcc
     5401 attgccgctt atgaagcaaa acaacctgcg tttatgaatt aatccccttg cccggtcaaa
     5461 tgaccgggct ttccgctatc gtccacgtca tcaaacgtct ttaacctttg cggttaaaaa
     5521 taatgcgaca acagaaataa ccgcaattac ccagtagaca gcaaagtgcc cccaactttg
     5581 tgcaattgct ccctgaataa cgccagccag aattaccccg gtagaaatac tgttagtaaa
     5641 taaggtggta gctgcccccg ctcttccagg cattaaatcc tgaaaccata gcatcccaat
     5701 acccgcaaca atgccgataa atacagcgtt aaaaagttgc agcgtcatca acgccatacg
     5761 gctatgaaag aaaatcaatc cggtgtaaaa cagtactcct gccgccactg ctatgaccat
     5821 cattcgccgc ttaccataac gtttgacata gtagccagcc agaatcattg ctggtatttc
     5881 cagtccagct gccgtcccca tcaggaaacc cgccagtttg tctggcaatc ctaactcgct
     5941 actgatccac aacggcatat caataatgta catggtgttg caggtccaca ttaacgtcga
     6001 ggcgacaaat aacatccgta cgttactatc ctgccagcca ccttgcattg ataaagcatt
     6061 ttccgacggc agttctaccc gcgccacaga cggaagcata aatgcaatca ataccagact
     6121 gagtgtgaat atcccggcgg caatcgaaaa catcaccact tctcgcgccg agttatcctc
     6181 atattcccgc gccagagcaa ataactgtgg cattgccgta ttggccagag atgccagaag
     6241 cacaccacag gtgataagcg tcagataatg acgattaaat gcaaacaatg gcgcattgcc
     6301 gatagccatc aaacagcaaa atataatcag ttttcgccga tcgccctgac tgtcagaacg
     6361 ttttgccaac cagaggctta cgccgatccc agcaatagta ttcaccgtat aaaagagtcc
     6421 gatccagaaa ggttgcgcgc caacctcacg actcagaaat aagctcaatg taggagcctg
     6481 tagcgccccg gccaccccca tcataaaagc gaccagcata aatgccgcgt aaacaccgtt
     6541 catacggcga gccatcgtca ttatccagat catacgttcc ctttttagcg cggcgagaat
     6601 aaaaaaacca gcaggtataa tctgctggcg ggtgatttta caccaatact cagtcacaca
     6661 tgatgcaggc aagtcaactt tcagaattgc ggtcatccca ctgcatcagt ccttcaagca
     6721 tggttaatcg ttgtgggacg cttaaccaac gcaaccagca caacttcgct gtcgcggtaa
     6781 aatagtgctg ataaaactga cgcatggggc acctccttgc ttcatcgtta ggaattattg
     6841 acttaatata gggaaaataa aattgctgtc ttttgcgcag gagttcccct tttatgccat
     6901 ctgctcgtct gcaacaacag ttcatccgcc tgtggcaatg ctgcgagggt aaatcgcagg
     6961 acacaacgct caacgaactg gcagcgttat tgagctgctc gcgtcgtcat atgcgtaccc
     7021 tgctcaacac catgcaggat cgcggctggc tgacgtggga agcggaagtc gggcgcggta
     7081 aacgctcgcg tctgacattc ctctataccg ggctggcgct tcagcaacag cgggcggaag
     7141 acctgctgga gcaggatcgt atcgaccaac tagtgcagtt ggttggcgac aaagcgactg
     7201 tgcggcaaat gctggtttct catctgggcc gcagcttccg ccaggggcgg cacatcctgc
     7261 gcgtgctcta ctatcgtccg ttgcgtaatc tgctacctgg cagcacattg cgccgttccg
     7321 aaacccatat tgcccggcaa atcttcagtt cgctaacgcg cataaatgag gaaaatgggg
     7381 aactggaagc agacatcgcc caccactggc agcaaatatc accgcttcac tggcgtttct
     7441 ttttgcgtcc aggagtccat tttcatcatg gtcgtgaact ggaaatggac gacgtgatcg
     7501 cctctttaaa acgaatcaat acgctgccgc tctattcgca tattgcggac attgtgtcgc
     7561 cgacgccctg gacgctggat atccatctca cgcaaccgga ccgctggtta ccgttactgc
     7621 tggggcaagt tccggcgatg atcctgccgc gcgaatggga aaccctcagt aactttgcca
     7681 gccatcccat cggcaccggt ccgtatgcgg tgatccgcaa caccaccaat caactgaaaa
     7741 ttcaggcatt cgatgacttc ttcggttacc gggcattaat cgatgaagtt aacgtctggg
     7801 ttctgccgga aattgccgac gagccagctg gaggtctgat gctaaaaggt ccacagggtg
     7861 aggaaaaaga gattgaaagc cgcctggagg aaggttgcta ctatttacta ttcgacagcc
     7921 gcacccatcg cggagcgaat cagcaagtca gggactgggt aaactatgtg ctttctccaa
     7981 ctaatctggt ctatttcgct gaggaacagt accagcaact gtggttcccg gcttatggac
     8041 tgctcccccg ttggcaccat gcccgcacca taaagagcga aaaaccggct ggcctggaaa
     8101 gcctcaccct gaccttttat caggatcaca gtgagcatcg ggtgattgcc gggatcatgc
     8161 agcagattct ggcaagtcac caggtcacgc tggaaatcaa agagatcagc tacgatcagt
     8221 ggcatgaagg agagatcgag agcgatatct ggcttaatag cgccaacttt acgctgccgc
     8281 tggatttttc gctgttcgcg cacttgtgcg aggtgccgct gctccaacac tgtattccga
     8341 tcgactggca agccgacgcc gcccgctggc gcaatggcga aatgaacctg gcgaactggt
     8401 gccagcaact ggtcgccagt aaagcaatgg tgccgcttat ccaccactgg ctgatcattc
     8461 agggacaacg cagtatgcgc ggcctgcgta tgaataccct cggctggttc gattttaaat
     8521 cagcgtggtt tgcgccgccg gatccatgat tgctggtaga taacaaaatc actacactaa
     8581 cgccgttctc aacggggtgc cacgcaaacg cgtgcgctga gaaaataccc gtcgaacctg
     8641 atccggataa cgccggcgaa gggatttgag gctccttctc aagtcctttg ccactctttt
     8701 ttgaggtgca aagtgttaaa aaaatgtctt cccctgctgt tgctgtgcac agcgcccgtt
     8761 ttcgctaaac ccgttctgac tgtttatacc tacgattcct tcgccgccga ctgggggcct
     8821 ggtccaaaga tcaaaaaagc ttttgaagcc gactgtaact gcgaactgaa actggtggcg
     8881 ctggaagatg gcgtttcgct tctcaaccgt ctacggatgg aaggcaaaaa tagtaaagcc
     8941 gatgtggtgc tggggctgga taacaatctg ttagacgccg ccagtaaaac cggactattt
     9001 gccaaaagcg gtgtggcagc ggatgccgtt aacgttcccg gcggctggaa taatgacact
     9061 ttcgtcccgt ttgattacgg ctatttcgcc ttcgtctatg acaagaacaa actgaaaaac
     9121 ccgccgcaaa gcctgaaaga attggttgag agcgatcaaa actggcgggt gatttatcag
     9181 gatccgcgca ccagtacgcc ggggctgggc ctgctgttgt ggatgcaaaa agtctatggc
     9241 gatgacgccc cacaagcctg gcaaaaactg gcgaagaaaa ccgtcacggt caccaaaggc
     9301 tggagcgaag cctacggcct gtttttaaaa ggtgaaagcg atctggtact gagttacacc
     9361 acctctccgg cttatcacat tctcgaagag aaaaaagata actacgccgc cgcgaacttt
     9421 agcgaaggtc actatctgca ggtggaagtc gccgcccgca ccgctgccag caagcagccg
     9481 gagctggcgc aaaaattcct ccagtttatg gtttctccgg cttttcagaa tgcgatccca
     9541 accggtaact ggatgtatcc ggtggcaaat gtcacgctgc ctgccgggtt tgaacaattg
     9601 accaaaccgg ccaccacgct ggagttcaca ccagctgaag tggcggcaca acgtcaggca
     9661 tggattagcg gatggcaacg cgccgtcagc cgttaattcc cggctggtta attccaggtg
     9721 taagcgccgc cacgctggtg gtagcggttg cgctcgcggc gtttctcgcc ctgtggtgga
     9781 acgcgccgca gggtaactgg gtggcagtct ggcaggacag ctacctgtgg catgtggtgc
     9841 gcttctcctt ctggcaggcg tttctctcgg cgctactctc tgtcgtaccc gcgatattcc
     9901 tcgcccgcgc cctctatcgc aggcgctttc cgggtcggct ggcgctgttg cgtctgtgcg
     9961 caatgacctt gatcctcccg gtgctggttg ctgttttcgg cattcttagc gtctatggtc
    10021 gccagggctg gctggcatcg ctctgccaat cgctcggtct ggagtggacc ttttcgccct
    10081 acggcctgca aggtattttg ctggcgcacg tattttttaa tctgccgatg gcgagccgct
    10141 tattactcca ggcactggaa aacattcccg acgaacaacg tcagcttgcc gcccagcttg
    10201 ggatgcgcgg ctggcatttt ttccgcttcg tcgaatggcc gtggttacgg cgacaaatcc
    10261 cgccggttgc tgcacttatc tttatgctct gtttcgccag cttcgccacc gtgctatcac
    10321 tgggcggcgg tccgcaggcg accactatcg agctggctat ttatcaggcg ctgagttacg
    10381 actacgatcc tgcccgcgcg gcgatgctgg cgctgatcca gatggtgtgt tgccttgggc
    10441 tggtactgtt gagtcagcga ttgagtaagg ccattgcgcc aggcaccacg ctgctgcaag
    10501 gctggcgcga cccggacgat cgtctgcata gccgcatttg cgacacggtg ttaatagtgc
    10561 tggcgttgct gctgttgttg ccaccgttgc tggcggtgat cgtcgatggg gtaaatcgcc
    10621 agttgccgga agtgctggca caaccggtgc tgtggcaggc gctgtggacc tcgttgcgta
    10681 ttgcgctggc ggcaggtgta ttgtgcgtag tgctgaccat gatgctgcta tggagcagtc
    10741 gcgaacttcg ggcacggcag aaaatgctgg cgggtcaggc gctggagatg agcggcatgt
    10801 tgattctcgc catgccgggg attgtgctgg ctaccggctt ctttttactg ctcaacaaca
    10861 ctatcggcct gcaacaatct gctgacggca ttgtgatttt caccaatgcg ttaatggcga
    10921 tcccttatgc gctgaaagta ctggaaaacc cgatgcgcga tatcaccgcc cgctacagta
    10981 tgttgtgtca gtcgctgggc attgaaggct ggtcgcgctt aaaagtggtg gagctgcgcg
    11041 ccctgaaacg tccactggcg caggcgctgg cctttgcctg cgtgctgtcg attggtgatt
    11101 ttggcgtggt ggcgttgttc ggtaacgatg atttccgtac cctgccgttt tatctctacc
    11161 agcaaattgg ctcctatcgc agccaggacg gcgcggtcac cgcgttaatt ctgctgctac
    11221 tctgttttct gctgtttacc gtgattgaaa aactaccggg gcgaaatgtt aaaactgact
    11281 gatatcacct ggctttacca acatttgccg atgcgtttta gcttaacggt ggaacgcggc
    11341 gagcaggtgg cgatcctcgg gccaagcggc gcaggtaaaa gtaccctgct gaatttgatt
    11401 gccggttttc tgacgccagc cagcggttcg ctgactatcg atggcgttga tcacaccacg
    11461 acgccgccgt cacgccgtcc ggtgtcgatg ctgtttcagg agaacaacct gttcagccac
    11521 ctgacggtcg cgcagaacat cgggctgggg cttaatccag gattgaagct gaacgcggca
    11581 cagcaggaga aaatgcacgc tatcgcccgt cagatgggga ttgataattt aatggcgcgg
    11641 ttaccgggcg agctttccgg cggccagcga cagcgcgtgg cgttagcgcg ttgtctggtg
    11701 cgcgaacagc cgattttatt gctcgatgaa ccgttctctg cgctcgatcc ggcgttacgt
    11761 caggagatgc tgacgctggt gagcacgagc tgccagcagc aaaaaatgac gctattgatg
    11821 gtgtcgcaca gtgtggaaga tgcggcgcgg atcgccacac gctcggtggt ggtcgccgac
    11881 gggcgcatcg cctggcaggg taagactgaa gaattgttga gcggtaaggc gagtgcttcg
    11941 gcaatattgg ggattacggg ttagtatgca ctgtcagatg cggcgtgaac accttatccg
    12001 acctacggac agtgcgtttt gtaggcctga taagaccggt cagcgtcgca tcaggcaacc
    12061 cgcacaagac aatcccctaa accccaacca ctttacgcaa aatatcgata tacaccggca
    12121 tcaacgggtg gcgaattaac accaccagcg ccaccacgcc gatggcagaa atcaacggcg
    12181 tcagccacaa caaacggccg cgcgacaaat aatgacttaa acgatcggtt gctttaccgc
    12241 tgcgccataa ccgccagcac agccagccac caacccacaa aaataccgcc gtcgccagca
    12301 gcaaccattt aaactcgccg ctctgcattc cggctggaat atcgatcgcc gcgcccgcca
    12361 gaatcccagg caggaagtaa aacggtggcc acagcaggca gccgataata ttcggcgtaa
    12421 tgaattttgc caccggcaga tccagcattc ccgccaccat tggcaccagc ggacgcgtcg
    12481 ggccaacaaa gcgaccgacc agaatggtga acatgctgtg ttgatgcaac gcatgttcag
    12541 ttttatcgag taatgctttg tttttcttca gaaatgacca gcgatgcaac ggctttttaa
    12601 aacgccagcc cagccagaag gagatccagt cgcccagcag acaaccgaca atccctgcca
    12661 gccaggcgtg ccagaaactt aactcgccgc tgccaatcag cgctcccagc cccgccatca
    12721 gcaccgtacc gggtagaatc aaaccgacca gcgccagcga ctccagaaag gccaccaaca
    12781 ccaccgccat caatgaatac acggtagatt gggtaataaa gtgttccagc aatgcttgca
    12841 taatgtgcct gtcaaatgga cgaagcaggg attctgcaaa ccctatgcta ctccgtcaag
    12901 ccgtcaattg tctgattcgt taccaattat gacaacttga cggctacatc attcactttt
    12961 tcttcacaac cggcacggaa ctcgctcggg ctggccccgg tgcatttttt aaatacccgc
    13021 gagaaataga gttgatcgtc aaaaccaaca ttgcgaccga cggtggcgat aggcatccgg
    13081 gtggtgctca aaagcagctt cgcctggctg atacgttggt cctcgcgcca gcttaagacg
    13141 ctaatcccta actgctggcg gaaaagatgt gacagacgcg acggcgacaa gcaaacatgc
    13201 tgtgcgacgc tggcgatatc aaaattgctg tctgccaggt gatcgctgat gtactgacaa
    13261 gcctcgcgta cccgattatc catcggtgga tggagcgact cgttaatcgc ttccatgcgc
    13321 cgcagtaaca attgctcaag cagatttatc gccagcagct ccgaatagcg cccttcccct
    13381 tgcccggcgt taatgatttg cccaaacagg tcgctgaaat gcggctggtg cgcttcatcc
    13441 gggcgaaaga aacccgtatt ggcaaatatt gacggccagt taagccattc atgccaatag
    13501 gcgcgcggac gaaagtaaac ccactggtga taccattcgc gagcctccgg atgacgaccg
    13561 tagtgatgaa tctctcctgg cgggaacagc aaaatatcac ccggtcggca aacaaattct
    13621 cgtccctgat ttttcaccac cccctgaccg cgaatggtga gattgagaat ataacctttc
    13681 attcccagcg gtcggtcgat aaaaaaatcg agataaccgt tggcctcaat cggcgttaaa
    13741 cccgccacca gatgggcatt aaacgagtat cccggcagca ggggatcatt ttgcgcttca
    13801 gccatacttt tcatactccc gccattcaga gaagaaacca attgtccata ttgcatcaga
    13861 cattgccgtc actgcgtctt ttactggctc ttctcgctaa cccaaccggt aaccccgctt
    13921 attaaaagca ttctgtaaca aagcgggacc aaagccatga caaaaacgcg taacaaaagt
    13981 gtctataatc acggcagaaa agtccacatt gattatttgc acggcgtcac actttgctat
    14041 tccatagcat ttttatccat aagattagcg gatcctacct gacgcttttt atcgcaactc
    14101 tctactgttt ctccataccc gtttttttgg atggagtgaa acgatggcga ttgcaattgg
    14161 cctcgatttt ggcagtgatt ctgtgcgagc tttggcggtg gactgcgcca gcggtgaaga
    14221 gatcgccacc agcgtagagt ggtatccccg ttggcaaaaa gggcaatttt gtgatgcccc
    14281 gaataaccag ttccgtcatc atccgcgtga ctacattgag tcaatggaag cggcactgaa
    14341 aaccgtgctt gcagagctta gcgtcgaaca gcgcgcagct gtggtcggga ttggcgttga
    14401 cagtaccggc tcgacgcccg caccgattga tgccgacggt aacgtgctgg cgctgcgccc
    14461 ggagtttgcc gaaaacccaa acgcgatgtt cgtattgtgg aaagaccaca ctgcggttga
    14521 agaagcggaa gagattaccc gtttgtgcca cgcgccgggc aacgttgact actcccgcta
    14581 cattggtggt atttattcca gcgaatggtt ctgggcaaaa atcctgcatg tgactcgcca
    14641 ggacagcgcc gtggcgcaat ctgccgcatc gtggattgag ctgtgcgact gggtgccagc
    14701 tctgctttcc ggtaccaccc gcccgcagga tattcgtcgc ggacgttgca gcgccgggca
    14761 taaatctctg tggcacgaaa gctggggcgg cttgccgcca gccagtttct ttgatgagct
    14821 ggacccgatc ctcaatcgcc atttgccttc cccgctgttc actgacacct ggactgccga
    14881 tattccggtg ggcaccttat gcccggaatg ggcgcagcgt ctcggcctgc ctgaaagcgt
    14941 ggtgatttcc ggcggcgcgt ttgactgcca tatgggcgca gttggcgcag gcgcacagcc
    15001 taacgcactg gtaaaagtta tcggtacttc cacctgcgac attctgattg ccgacaaaca
    15061 gagcgttggc gagcgggcag ttaaaggtat ttgcggtcag gttgatggca gcgtggtacc
    15121 tggatttatc ggtctggaag caggccaatc ggcgtttggt gatatctacg cctggtttgg
    15181 tcgcgtactc ggctggccgc tggaacagct tgccgcccag catccggaac tgaaagcgca
    15241 aatcaacgcc agccagaaac aactgcttcc ggcgctgacc gaagcatggg ccaaaaatcc
    15301 gtctctggat cacctgccgg tggtgctcga ctggtttaac ggccgccgca caccgaacgc
    15361 taaccaacgc ctgaaagggg tgattaccga tcttaacctc gctaccgacg ctccgctgct
    15421 gtttggcggt ttgattgctg ccaccgcctt tggcgcacgc gcaatcatgg agtgctttac
    15481 cgatcagggg atcgccgtca ataacgtgat ggcgctgggc ggcatcgcgc ggaaaaacca
    15541 ggtcattatg caggcctgct gcgacgtgct gaatcgcccg ctgcaaattg ttgcctctga
    15601 ccagtgctgt gcgctcggtg cggcgatttt tgctgccgtc gccgcgaaag tgcacgcaga
    15661 catcccatca gcccagcaaa aaatggccag tgcggtagag aaaaccctgc aaccgcgcag
    15721 cgaacagaca caacgctttg aacagcttta tcgccgctat cagcaatggg cgatgagcgc
    15781 cgaacaacac tatcttccaa cttccgcccc ggcacaggct gcccaggccg ttccgactct
    15841 ataaggacac gataatgacg atttttgata attatgaagt gtggtttgtc attggcagcc
    15901 agcatctgta tggcccggaa accctgcgtc aggtcaccca acatgccgag cacgttgtta
    15961 atgcgctgaa tacggaagcg aaactgccct gcaaactggt gttgaaaccg ctgggcacca
    16021 cgccggatga aatcaccgct atttgccgcg acgcgaatta cgacgatcgt tgcgctggtc
    16081 tggtggtgtg gctgcacacc ttctccccgg ccaaaatgtg gatcaacggc ctgaccatgc
    16141 tcaacaaacc gttgctgcaa ttccacaccc agtttaacgc ggcgctgccg tgggacagta
    16201 tcgatatgga ctttatgaac ctgaaccaga ctgcacatgg cggtcgcgag ttcggcttca
    16261 ttggcgcgcg tatgcgtcag caacatgccg tggttaccgg tcactggcag gataaacaag
    16321 cccatgagcg tatcggctcc tggatgcgtc aggcggtctc taaacaggat acccgtcatc
    16381 tgaaagtctg ccgttttggc gataacatgc gtgaagtagc ggtcaccgat ggtgataaag
    16441 ttgccgcaca gatcaagttc ggtttctccg tcaatacctg ggcggttggc gatctggtgc
    16501 aggtggtgaa ctccatcagc gacggcgatg ttaacgcgct ggtcgatgag tacgaaagct
    16561 gctacaccat gacgcctgcc acacaaatcc acggcgaaaa acgacagaac gtgctggaag
    16621 cggcgcgtat tgagctgggg atgaagcgtt tcctggaaca aggtggcttc cacgcgttca
    16681 ccaccacctt tgaagatttg cacggtctga aacagcttcc aggtctggcc gtacagcatc
    16741 tgatgcagca gggttacggc tttgcgggcg aaggcgactg gaaaaccgcc gccctgcttc
    16801 gcatcatgaa ggtgatgtca accggtctgc agggcggcac ctcctttatg gaggactaca
    16861 cctatcactt cgagaaaggt aatgacctgg tgctcggctc ccatatgctg gaagtctgcc
    16921 cgtcgattgc cgtagaagag aaaccgatcc tcgacgttca gcatctcggt attggtggta
    16981 aggacgatcc tgcccgactg atcttcaata cccaaaccgg tccagcgatt gtcgccagcc
    17041 tgattgatct cggcgatcgt taccgtctgc tggttaactg tatcgacacg gtgaaaacac
    17101 cgcactccct gccgaaactg ccggtggcga atgcgctgtg gaaagcgcaa ccggatctgc
    17161 caactgcttc cgaagcgtgg atcctcgctg gtggcgcgca ccataccgtc ttcagccatg
    17221 cgctgaacct caacgatatg cgtcagttcg ccgagatgca cgacattgaa atcacggtga
    17281 ttgataacga cacccgcctg ccagcgttta aagacgcact gcgctggaac gaagtgtatt
    17341 acggatttcg tcgctaagta gccacatcag gtatgtaacg cctgatgcga cgctgacgcg
    17401 tcttatctgg cctacacgct gcgattttgt aggccggata agcaaagcgc atccggcatt
    17461 caacgcctga tgcgacgctg gcgcgtctta tcaggcctac gcgctgcgat tttgtagacc
    17521 ggataagcga agcgcatccg gcacgaagga gtcaacatgt tagaagatct caaacgccag
    17581 gtattagaag ccaacctggc gctgccaaaa cacaacctgg tcacgctcac atggggcaac
    17641 gtcagcgccg ttgatcgcga gcgcggcgtc tttgtgatca aaccttccgg cgtcgattac
    17701 agcgtcatga ccgctgacga tatggtcgtg gttagcatcg aaaccggtga agtggttgaa
    17761 ggtacgaaaa agccctcctc cgacacgcca actcaccggc tgctctatca ggcattcccc
    17821 tccattggcg gcattgtgca tacgcactcg cgccacgcca ccatctgggc gcagtcgggt
    17881 cagtcgattc cagcaaccgg caccacccac gccgactatt tctacggcac cattccctgc
    17941 acccgcaaaa tgaccgacgc agaaatcaac ggcgaatatg agtgggaaac cggtaacgtc
    18001 atcgtagaaa cctttgaaaa acagggtatc gatgcagcgc aaatgcccgg cgttctggtc
    18061 cattcccacg gcccgtttgc atggggcaaa aatgccgaag atgcggtgca taacgccatc
    18121 gtgctggaag aggtcgctta tatggagata ttctgccgtc agttagcgcc gcagttaccg
    18181 gatatgcagc aaacgctgct ggataaacac tatctgcgta agcatggcgc gaaggcatat
    18241 tacgggcagt aatgactgta taaaaccaca gccaatcaaa cgaaaccagg ctatactcaa
    18301 gcctggtttt ttgatggatt ttcagcgtgg cgcaggcagg ttttatctta acccgacact
    18361 ggcgggacac cccgcaaggg acagaagtct ccttctggct ggcgacggac aacgggccgt
    18421 tgcaggttac gcttgcaccg caagagtccg tggcgtttat tcccgccgat caggttcccc
    18481 gcgctcagca tattttgcag ggtgaacaag gctttcgcct gacaccgctg gcgttaaagg
    18541 attttcaccg ccagccggtg tatggccttt actgtcgcgc ccatcgccaa ttgatgaatt
    18601 acgaaaagcg cctgcgtgaa ggtggcgtta ccgtctacga ggccgatgtg cgcccgccag
    18661 aacgctatct gatggagcgg tttatcacct caccggtgtg ggtcgagggt gatatgcaca
    18721 atggcactat cgttaatgcc cgtctgaaac cgcatcccga ctatcgtccg ccgctcaagt
    18781 gggtttctat agatattgaa accacccgcc acggtgagct gtactgcatc ggcctggaag
    18841 gctgcgggca gcgcatcgtt tatatgctgg ggtcggagaa tggcgacgcc tcctcgcttg
    18901 atttcgaact ggaatacgtc gccagccgcc cgcagttgct ggaaaaactc aacgcctggt
    18961 ttgccaacta cgatcctgat gtgatcatcg gttggaacgt ggtgcagttc gatctgcgaa
    19021 tgctgcaaaa acatgccgag cgttaccgtc ttccgctgcg tcttgggcgc gataatagcg
    19081 agctggagtg gcgcgagcac ggctttaaaa acggcgtctt ttttgcccag gctaaaggtc
    19141 ggctaattat cgacggtatc gaggcgctga aatccgcgtt ctggaatttc tcttcattct
    19201 cgctggaaac tgtcgctcag gagctattag gcgaaggaaa atctatcgat aacccgtggg
    19261 atcgaatgga cgaaattgac cgccgtttcg ccgaagataa acctgcgctg gcaacttata
    19321 acctgaaaga ttgcgagctg gtgacgcaga tcttccacaa aactgaaatc atgccatttt
    19381 tactcgaacg ggcaacggtg aacggcctgc cggtggaccg acacggcggt tcggtggcgg
    19441 catttggtca tctctatttt ccgcgaatgc atcgcgctgg ttatgtcgcg cctaatctcg
    19501 gcgaagtgcc gccgcacgcc agccctggcg gctacgtgat ggattcacgg ccagggcttt
    19561 atgattcagt gctggtgctg gactataaaa gcctgtaccc gtcgatcatc cgcacctttc
    19621 tgattgatcc cgtcgggctg gtggaaggca tggcgcagcc tgatccagag cacagtaccg
    19681 aaggttttct cgatgcctgg ttctcgcgag aaaaacattg cctgccggag attgtgacta
    19741 acatctggca cgggcgcgat gaagccaaac gccagggtaa caaaccgctg tcgcaggcgc
    19801 tgaaaatcat catgaatgcc ttttatggcg tgctcggcac caccgcctgc cgcttcttcg
    19861 atccgcggct ggcatcgtcg atcaccatgc gtggtcatca gatcatgcgg caaaccaaag
    19921 cgttgattga agcacagggc tacgacgtta tctacggcga taccgactca acgtttgtct
    19981 ggctgaaagg cgcacattcg gaagaagaag cggcgaaaat cggtcgtgca ctggtgcagc
    20041 acgttaacgc ctggtgggcg gaaacgctgc aaaaacaacg gctgaccagc gcattagaac
    20101 tggagtatga aacccatttc tgccgttttc tgatgccaac cattcgcgga gccgataccg
    20161 gcagtaaaaa gcgctacgcc gggctgattc aggagggcga taaacagcgg atggtgttta
    20221 aagggctgga aaccgtgcgc accgactgga cgccgctggc ccagcagttt cagcaggagc
    20281 tatacctgcg catcttccgc aacgagccat atcaggaata tgtacgcgaa accatcgaca
    20341 aactgatggc gggtgaactg gatgcgcgac tggtttaccg taaacgcctt cgccgtccgc
    20401 tgagcgagta tcagcgtaat gtgccgcctc atgtacgcgc cgctcgcctt gccgatgaag
    20461 aaaaccaaaa gcgtggtcgc cccttgcaat atcagaatcg cggcaccatt aagtacgtat
    20521 ggaccaccaa cggcccggag ccgctggact accaacgttc accactggat tacgaacact
    20581 atctgacccg ccagctacaa cccgtggcgg agggaatact cccttttatt gaggataatt
    20641 ttgctacact tatgaccggg caacttgggc tattttgagc aaaaaaagag ttcgccagat
    20701 accattttga tgcgtgacga atgctttgcc atccagtacc atagcgccct ttccattcct
    20761 ggacctgaat aacaccacta cctcataagc acggtagcgg gtggttattg cctgcaatta
    20821 aagatataga gccgaacaca tatgcctttt acacttggtc aacgctggat cagcgataca
    20881 gaaagcgaat tgggacttgg aaccgttgtc gcggtggatg cgcgaactgt cactttactt
    20941 ttcccatcta ctggtgaaaa ccgtctgtac gcacgcagtg attcccccgt gacccgcgtg
    21001 atgttcaacc ctggtgatac cattaccagc catgacggct ggcagatgca agtcgaagaa
    21061 gtaaaagaag aaaatggctt gctgacctat atcggtactc gcctggatac tgaagagtcc
    21121 ggcgtagccc tgcgtgaagt tttccttgat agcaaactgg tgttcagcaa accgcaggac
    21181 cgcctgtttg ccgggcagat tgaccgtatg gaccgctttg cgctgcgtta tcgcgcgcgt
    21241 aagtattcca gcgaacagtt ccgtatgccg tacagcggcc tgcgcggtca gcgtaccagc
    21301 ctgatcccgc accagctcaa catcgctcat gatgttggcc gccgccacgc gccgcgcgtc
    21361 ctgctggctg acgaagtggg tttagggaaa accattgaag ccgggatgat cctgcatcag
    21421 caactgctct ctggcgctgc tgaacgtgtg ctgattatcg tcccggaaac cttacagcat
    21481 cagtggctgg tagaaatgct gcgccgtttc aacctgcgct ttgctctgtt tgatgatgag
    21541 cgttatgccg aagctcagca cgatgcttac aacccgttcg acaccgaaca gctggtgatt
    21601 tgctcgctgg attttgcccg tcgtagcaaa cagcgcctgg aacatctctg tgaagccgaa
    21661 tgggacctgc tggtggtcga tgaagcgcat cacctggtgt ggagcgaaga tgcgccaagc
    21721 cgtgaatatc aggccattga acaactggca gagcacatgc cgggcgttct gctgctgacc
    21781 gcgaccccgg aacagctggg gatggaaagc cacttcgccc gtctgcgtct gctggacccg
    21841 aaccgtttcc acgattttgc gcagttcgtt gaagagcaga aaaattatcg tccggttgcg
    21901 gacgccgttg ccatgctgct ggcaggtaac aaactgagca atgacgaact gaacatgctc
    21961 ggcgagatga tcggcgagca ggatatcgag ccgctgttgc aggcagcaaa cagcgacagc
    22021 gaagatgccc agagcgcccg tcaggagctg gtttcgatgc tgatggatcg ccacggcacc
    22081 agccgcgtgc tgttccgtaa cacgcgtaac ggtgtgaaag gattcccgaa acgcgagctg
    22141 cacaccatta agctgccgct accgacgcag tatcagacgg ctattaaagt ctccggcatt
    22201 atgggcgcac gtaaaagtgc ggaagatcgt gctcgcgata tgctctaccc ggagcgtatt
    22261 tatcaggaat ttgaaggtga taacgccacc tggtggaact tcgatccgcg cgttgagtgg
    22321 ctgatgggct acctgaccag ccatcgctct cagaaagtgc tggtgatctg cgccaaagct
    22381 gccactgcgc tgcaactgga gcaggtactg cgcgaacgtg aaggtattcg cgctgcggtg
    22441 ttccacgaag gtatgtcgat tatcgaacgt gaccgcgctg ccgcctggtt tgccgaagaa
    22501 gacaccggcg cacaggtact gctgtgctca gaaatcggtt ctgaaggacg taacttccag
    22561 ttcgccagcc acatggtgat gtttgacctg ccattcaacc cggatctact ggagcagcgt
    22621 attggtcgtc tggatcgtat cggccaggcg cacgatattc agatccatgt gccttatctg
    22681 gagaaaaccg ctcagtcggt gctggtgcgc tggtatcacg aaggtctgga tgcatttgag
    22741 cacacctgcc cgaccggacg cactatttac gatagcgtat acaacgatct gattaactat
    22801 ctggcttcac cggatcaaac cgaaggcttt gacgatctga tcaaaaactg ccgcgagcaa
    22861 catgaagcgc tgaaagcaca gctggaacag ggtcgtgacc gcctgctgga aatccactcc
    22921 aacggtggcg aaaaagccca ggcactggca gaaagcattg aagagcagga tgacgatacc
    22981 aacctgatcg ccttcgccat gaacctgctc gatattatcg gtatcaatca ggacgatcgc
    23041 ggcgacaaca tgatcgtgct gacgccgtcc gatcatatgc tggtgccgga cttccctggc
    23101 ctgtcggaag atggcatcac catcaccttt gatcgtgaag tggcgctggc gcgtgaagat
    23161 gcacagttta ttacctggga gcatccgctg atccgcaacg gtctggatct gatcctttct
    23221 ggcgataccg gtagcagcac gatttcactg ttaaaaaaca aagcgttgcc ggtaggtacg
    23281 ctgttggtgg aactgattta tgtggttgaa gcccaggctc cgaagcagtt gcagctcaac
    23341 cgcttcctgc caccgacgcc ggtacgtatg ctgctggata aaaacggcaa caacctggcg
    23401 gcgcaggtag agtttgaaac ctttaaccgc cagcttaacg cggttaaccg tcacaccggc
    23461 agcaaactgg ttaacgccgt gcagcaggat gttcacgcta tccttcaact gggtgaagcg
    23521 cagatcgaga aatctgcccg tgcattgatt gatgcagcgc gtaacgaagc cgacgaaaaa
    23581 ctgtctgccg agctgtctcg tctggaagct ctgcgtgcag tgaacccgaa cattcgtgac
    23641 gacgaactga ccgccattga gagcaaccgt cagcaggtaa tggaaagcct ggatcaggca
    23701 ggttggcgtc tggatgccct gcgtttgatc gttgtaacgc atcagtaacg gagccgaaaa
    23761 tggggatgga aaactacaat ccaccgcagg aaccctggtt ggttatcctg tatcaggatg
    23821 accacattat ggtggttaac aagccgagcg gtttgttgtc tgtgccgggg cgtctggaag
    23881 agcacaaaga cagcgtgatg acgcgcattc agcgtgatta tccgcaggca gaatcggtgc
    23941 atcgtctgga tatggctacc agcggcgtga ttgtagtggc gctgaccaaa gccgcggagc
    24001 gggagttaaa acgccagttc cgcgagcgcg agccgaaaaa gcagtatgtg gcccgcgtct
    24061 gggggcatcc atcccctgcg gaaggtctgg tggatctgcc gctgatttgc gactggccaa
    24121 accgcccgaa acagaaagtc tgttacgaaa cgggtaaacc tgcgcagacg gaatatgaag
    24181 tggtggagta tgcggcggat aacacggcaa gagtggtgtt aaaaccgatt actgggcgtt
    24241 cgcatcaatt gcgtgtgcat atgctggcgc tggggcatcc gattctcggc gatcgttttt
    24301 atgcatcacc agaagcgaga gcgatggcac cgcgtttgtt gctacatgca gagatgttga
    24361 cgattaccca tccggcgtat ggcaatagta tgacgtttaa agcgccagcg gatttttaat
    24421 agtcacgctg gcgcagttcg gtaggcggat aaggcgttca cgccgaatcc ggcaattgtg
    24481 cgccgattgc ctgatgcgac gcgggccgcg tcttatcagg cctacagttt aaaacttatt
    24541 taaacccttt ctgctgcttt atcagctcat atgcctgctg aatttcctgc gctttctgct
    24601 tcgccatctc catcatctca ggcggcaaac ctttcgccac cagcttatcg ggatggtgtt
    24661 cactcatcag cttacggtag gctcgtttaa tggtggtcgc atcatccgtc ggcttcacac
    24721 ccagcacatt acaggcatct tccagcgtcg ggccacgctg cgcttgctgc cagttaccac
    24781 cgccagattg ctgctgataa ccgccgccaa actgtgcacc gccctgcatc atgcgcaaaa
    24841 actggtcaaa ctgggcgcgg gagatcccta attcttctgc aatgacatac agcaccgccc
    24901 gttcattcgg gtgcagtgaa ccatcagcaa acgctgcctg aatctggatc tccagaaaca
    24961 tacgaattaa gtcaaaacga ccaaagcaga cactgcgaaa ctggcgcatc ttttcgcgca
    25021 gcgggtaatt atctgatttt cccacccgga acgcgttttg cgccgcagta cgggaagcgc
    25081 catgaaggtt cattcggtcc atcagctggc tggcgatatg aatatcagcc tccgtgacgc
    25141 gacctttgga tttggttaaa tgccccatca cttcaaaagt ggtggcaaaa aacagcgcct
    25201 gacgctcacg ctggttggcg aaccacgcca ttttacggct acgggcttta tcaaacatat
    25261 ggccaattaa caggcctaac actacgcccc aaaagccgcc gcccatcagt aaggccacgg
    25321 ccacgccaat gatttttccc caatactgca tatattcccc aaatcgacac acggatatca
    25381 gggctatctc ccacaatata aaggtgcttt taccgttttc cggctgcagt caattgtggg
    25441 acatcgccta taatttgcat tatcatacct gtcattcaat gccgtgccta acaccactga
    25501 cgctattcgg acaggattaa cactagcgta gagatgacga gtacgttagt ctctgaccgt
    25561 ttgtcacgcg caacgttacc gatgatggaa caataaaatc aacgtatgaa aaaacgtatc
    25621 cccactctcc tggccaccat gattgccacc gccctttata gtcaacaggg actggcagcc
    25681 gacctcgcct cacagtgcat gttgggcgtg ccaagctatg accgtcctct ggtacagggc
    25741 gataccaatg acttacccgt gactatcaat gctgaccacg cgaaagggga ctacccggat
    25801 gacgccgtgt ttactggcag cgtggatatc atgcagggta acagccgtct gcaggccgac
    25861 gaagtgcagc tccatcaaaa agaggcacca ggacaaccgg agccggtacg aaccgttgat
    25921 gcgctcggta atgtccatta cgacgataac caggtgatcc tcaaagggcc gaaaggctgg
    25981 gcgaatctga acaccaaaga taccaacgtc tgggaaggtg attaccagat ggtgggtcgc
    26041 cagggccgcg gtaaagcgga cctgatgaaa caacgtggtg aaaaccgcta taccattctg
    26101 gataacggta gctttacctc ctgtctgccg ggttctgaca cctggagcgt ggtaggtagc
    26161 gaaattattc atgaccgcga agaacaagtt gcggagatct ggaacgcccg ctttaaggtg
    26221 ggtccagtac cgatctttta tagcccctat ttgcagttgc cggtgggtga caaacgtcgc
    26281 tctggtttct tgatcccgaa cgccaagtac accaccacca actactttga gttctacctg
    26341 ccatattact ggaacatcgc gccaaatatg gatgccacca tcacgccgca ttatatgcat
    26401 cgtcgtggca acatcatgtg ggagaacgaa ttccgctacc tctcccaggc gggcgctggc
    26461 ttgatggaac tggactatct gccttcagat aaagtctatg aagatgaaca cccgaacgat
    26521 gacagttcac gtcgttggtt gttctactgg caacactccg gggtcatgga tcaagtgtgg
    26581 cgtttcaacg tagactacac caaggtcagc gatcctagct acttcaatga tttcgataac
    26641 aagtacggtt ccagtactga cggctacgca acgcaaaaat tcagcgttgg ctatgcggtg
    26701 caaaacttca atgccaccgt ttcaaccaag cagttccagg tctttagcga gcagaacacc
    26761 agtagctact cggcagagcc gcagttagac gttaactact accagaatga tgttggtccg
    26821 tttgatacgc gtatttacgg ccaggcagtg cactttgtta acaccagaga cgacatgcct
    26881 gaagcaaccc gtgttcacct ggaaccgacc atcaatttgc cgctctctaa taactggggc
    26941 agcatcaata ccgaagcgaa gttgctggca acccattatc agcaaaccaa tcttgactgg
    27001 tataactcca gaaacacgac caagctggac gaatccgtta accgcgttat gccgcaattc
    27061 aaagttgacg gcaaaatggt ctttgaacgc gatatggaaa tgctggctcc gggttatacc
    27121 caaacgctgg aaccgcgcgc gcagtatttg tacgtgccgt atcgcgatca gagcgacatc
    27181 tataactacg actcgtctct actgcaatct gactactctg gcctgttccg ggaccggact
    27241 tacggcggtc ttgaccgtat tgcctccgct aaccaggtga cgaccggtgt cacatctcgc
    27301 atatatgatg atgctgccgt tgaacatttt aatatttccg ttggtcaaat ctactatttc
    27361 acggagtctc gcactggcga tgacaacata acatgggaga atgacgacaa aacgggctca
    27421 ctggtgtggg caggcgatac ttactggcgt atctccgagc gttggggatt gcgtggcggg
    27481 attcagtacg atacacgtct ggataacgta gcgaccagta actccagcat tgaataccgt
    27541 cgggatgaag accgtctggt acagctgaat taccgttacg ccagcccgga atatattcag
    27601 gctacgctgc ctaagtacta ttccactgct gagcaatata agaatggtat ttcgcaggta
    27661 ggtgctgtcg ccagctggcc aattgccgat cgttggtcca ttgttggggc ctactactac
    27721 gacaccaatg cgaacaagca agccgactct atgttaggtg tgcaatacag ctcctgctgc
    27781 tatgcaattc gcgtcggtta cgagcggaag ctgaacggtt gggataacga taaacaacat
    27841 gcggtatatg acaacgcaat cggctttaac atcgaacttc gcggcctgag ctccaactac
    27901 ggtctgggta cgcaagagat gctgcgttcg aacattctgc cgtatcaaaa cactttgtga
    27961 tttgttgatt taccacgtaa tccgcagtgc ggttaattga aatggaaaaa gtatgaagaa
    28021 ctggaaaacg ctgcttctcg gtatcgccat gatcgcgaat accagtttcg ctgcccccca
    28081 ggtagtcgat aaagtcgcag ccgtcgtcaa taacggcgtc gtgctggaaa gcgacgttga
    28141 tggattaatg cagtcggtaa aactgaacgc tgcgcaggca aggcagcagc ttcctgatga
    28201 cgcgacgctg cgccaccaaa tcatggaacg tttgatcatg gatcaaatca tcctgcagat
    28261 ggggcagaaa atgggagtga aaatctccga tgagcagctg gatcaggcga ttgctaacat
    28321 cgcgaaacag aacaacatga cgctggatca gatgcgcagc cgtctggctt acgatggtct
    28381 gaactacaac acctatcgta accagatccg caaagagatg attatctctg aagtgcgtaa
    28441 caacgaggtg cgtcgtcgca tcaccatcct gccgcaggaa gtcgaatccc tggcgcagca
    28501 ggtgggtaac caaaacgacg ccagcactga gctgaacctg agccacatcc tgatcccgct
    28561 gccggaaaac ccgacttctg atcaggtgaa cgaagcggaa agccaggcgc gcgccattgt
    28621 cgatcaggcg cgtaacggcg ctgatttcgg taagctggcg attgctcatt ctgccgacca
    28681 gcaggcgctg aacggcggcc agatgggctg gggccgtatt caggagttgc cgggtatctt
    28741 cgcccaggca ttaagcaccg cgaagaaagg cgacattgtt ggcccgattc gttccggcgt
    28801 tggcttccat attctgaaag ttaacgacct gcgcggcgaa agcaaaaata tctcggtgac
    28861 cgaagttcat gctcgccata ttctgctgaa accgtcgccg atcatgactg acgaacaggc
    28921 ccgtgtgaaa ctggaacaga ttgctgccga tatcaagagt ggtaaaacga cttttgctgc
    28981 cgcagcgaaa gagttctctc aggatccagg ctctgctaac cagggcggtg atctcggctg
    29041 ggctacacca gatattttcg atccggcctt ccgtgacgcc ctgacccgcc tgaacaaagg
    29101 tcaaatgagt gcaccggttc actcttcatt cggctggcat ttaatcgaac tgctggatac
    29161 ccgtaatgtc gataaaaccg acgctgcgca gaaggatcgt gcataccgca tgctgatgaa
    29221 ccgtaagttc tcggaagaag cagcaagctg gatgcaggaa caacgcgcca gcgcctacgt
    29281 taaaatcctg agcaactaat ggttaaaacc caacgtgttg tgatcactcc cggcgagccc
    29341 gccgggattg gcccggactt agttgtccag cttgcacagc gtgagtggcc ggtcgaactg
    29401 gttgtttgtg ccgatgccac tctccttacc gaccgggcag cgatgctcgg tttgccgctc
    29461 accctccgcc cttattcccc caactcccct gcacaaccgc aaactgcggg cacattaacg
    29521 ctacttcctg tcgcgctacg cgaatctgtc actgcggggc agttagcgat tgaaaatggg
    29581 cattacgtgg tggagacgct ggcgcgagcg tgtgatggct gtctgaacgg tgaatttgct
    29641 gcgctgatca caggccccgt gcataaaggc gtcattaacg acgcaggtat tccgtttact
    29701 ggtcataccg agtttttcga agagcgctcg caggcgaaaa aagtggtgat gatgctggcg
    29761 accgaagaac ttcgcgtggc gctggcaacg acgcatttac cgctgcgcga tatcgcagat
    29821 gctatcaccc ctgcactttt gcacgaagtg attgctattt tgcatcacga tttgcgtaca
    29881 aaatttggta ttgccgaacc gcgcattctg gtctgcgggc tgaatccgca cgcgggcgaa
    29941 ggcggtcata tgggtacgga agagatagac accattattc cggtgctcga cgagctgcgg
    30001 gcgcagggga tgatactcaa cgggccgctg cctgccgata ccctgtttca gccgaaatat
    30061 ctcgataacg ccgacgccgt gctggcgatg taccacgatc agggtcttcc cgtgctaaaa
    30121 taccagggct tcgggcgcgg tgtgaacatt acgctgggcc tgccctttat tcgcacatca
    30181 gtggaccacg gcaccgcgct tgaactggcg ggacgtggcg aagccgatgt cggcagtttt
    30241 attacggcgc ttaatctcgc catcaaaatg attgttaaca cccaatgaat aatcgagtcc
    30301 accagggcca cttagcccgt aaacgcttcg ggcaaaactt tctcaacgat cagttcgtga
    30361 tcgacagcat tgtttctgcc attaacccgc aaaagggcca ggcaatggtc gaaatcggcc
    30421 ccggtctggc ggcattgacc gagccggtcg gcgaacgtct ggaccagctg acggtcatcg
    30481 aacttgaccg cgatctggcg gcacgtctgc aaacgcatcc attcttaggc ccgaaactga
    30541 cgatttatca gcaggatgcg atgaccttta actttggcga actggccgag aaaatgggtc
    30601 agccgctgcg tgttttcggc aacctgcctt ataacatctc cacgccgttg atgttccatc
    30661 tgtttagcta tactgatgcc attgccgaca tgcactttat gttgcaaaaa gaggtggtga
    30721 atcgtctggt tgcaggaccg aacagcaaag cgtatggtcg attaagcgtc atggcgcaat
    30781 actattgcaa cgtgatcccg gtactggaag ttccgccgtc agcctttaca ccaccaccca
    30841 aagtggattc cgccgtcgtg cgcctggttc ctcatgcaac gatgcctcac ccggttaaag
    30901 atgttcgtgt gttgagccgc atcaccaccg aagcctttaa ccagcgtcgt aaaaccattc
    30961 gtaacagcct cggcaacctg tttagcgtcg aggtgttaac gggaatgggg atcgacccgg
    31021 cgatgcgagc ggaaaatatc tctgtcgcgc aatattgcca gatggcgaac tatctggcgg
    31081 agaacgcgcc tttgcaggag agttaacgat gatcaattcg ccccgagtgt gtattcaggt
    31141 tcaaagcgtc tacattgagg cacaatcttc acctgataat gaacgttacg tttttgctta
    31201 taccgtaacc atacgcaatc tggggcgagc gccagtgcag ttgttggggc gttactggct
    31261 gatcaccaat ggcaatggcc gtgaaaccga agtccagggc gaaggagtgg ttggcgtcca
    31321 gccacttatc gcgcctggcg aagagtacca gtacaccagc ggtgcaatca ttgaaacccc
    31381 gctgggcacc atgcagggtc actacgaaat gatcgatgaa aatggcgtcc ctttcagcat
    31441 cgacattccc gtattccgac tcgccgttcc cacactcatt cattaaaaga atatggcgac
    31501 ataccttatt ggcgacgttc atggttgtta cgatgaactg atcgcattgc tgcataaagt
    31561 agaatttacc cctgggaaag ataccctctg gctgacgggc gatctggtcg cgcgcggccc
    31621 gggttcgctg gatgttctgc gctatgtgaa atccttaggc gacagcgtac gtctggtgct
    31681 gggcaatcac gatctgcatc tgctggcggt atttgccggg atcagccgca ataaaccgaa
    31741 agatcgcctg acaccgctgc tggaagcgcc ggatgccgac gagctgctta actggctgcg
    31801 tcgccagcct ttgctgcaaa tcgacgaaga gaaaaagttg gtgatggccc acgcagggat
    31861 cacgccgcag tgggatctac agaccgccaa agagtgcgcg cgcgatgtag aagcggtgct
    31921 atcgagtgac tcctatccct tctttctcga cgctatgtac ggcgatatgc caaataactg
    31981 gtcaccggaa ttgcgggggc tgggaagact gcgttttatc accaacgcct ttacccgtat
    32041 gcgtttttgc ttcccgaacg gtcaactgga tatgtacagc aaagaatcgc cggaagaggc
    32101 ccctgcccca ctgaaaccgt ggtttgcgat tcctggccct gtcgctgaag aatacagcat
    32161 cgcctttggt cactgggcat cgctggaggg caaaggtacg ccggaaggta tatacgcgct
    32221 ggataccggc tgctgctggg gtggtacatt aacctgcctg cgctgggaag ataaacagta
    32281 ttttgtccag ccgtcgaacc ggcataagga tttgggcgaa gcggcggcgt cttaaacaca
    32341 gcctgatata ggaaggccgg ataagacgcg accggcgtcg catccggcac tagccgtaaa
    32401 ttctatacaa aattaccgcc gctccagaat ctcaaagcaa tagctgtgag agttctgcgc
    32461 atcagcatcg tggaattcgc tgaataccga ttcccagtca tccggctcgt aatccgggaa
    32521 atgggtgtcg ccttccactt ctgcgtcgat atgcgtcaga tacagtttct gcgcttttgg
    32581 caggaactgc tcataaacgc gaccgccgcc aatcaccatg atttctggta cgtcaccaca
    32641 cgccgcgatg gcttcatcca ccgacttcac ccacgttacg cgatcgtccg tacccggttg
    32701 actgctgagg ataatatttt tgcgtcctgg caacggacga ccgattgatt cccaggtatg
    32761 gcggcccata atcacgggtt tatttaaggt gttgcgttta aaccaggcga gatcggcagg
    32821 caggttccac ggcatggcgt tttccatgcc gataacgcga tctaccgcta acgccgcaat
    32881 cagactgatc attgagattt cccgataaaa aaaattgtcg ccactatacg taaagcgtaa
    32941 accgtcgtcg actggtgcga ggatgatgtt gaggaaaatt ttatattctg ctggcgagtc
    33001 cacgctctct ccctggactc gccgcattac aatgaaacaa aaacaaacag ttagctgtaa
    33061 agtgtgattt atgtcactct ttattaggat gagggtttcg tttccggttc atccgccatg
    33121 ttgccggtat gtttaccttc ttcggttccc tgccagccat gtcgttgaat taatgacaga
    33181 tgttcgcggt cctcggtaat gatctcactt aacatcgcgc tggtgcgttt atagaccgcc
    33241 gcgcgggctt tggtgtcgtt ctcaaccatt gccatctctt ccaccatctg aatattaaag
    33301 cggcggaaca catcggcacg ttctcgcgct tcatacggcc ccagacctaa actttccagt
    33361 gccagacgcc cggttttcag cgcaccttcg aaggtttcac gctccggctt ttcaacgcct
    33421 gcctgacgca aacgaatgta gtggtcgaca tcgcgggcgc gggcaataat ctgcaaatgc
    33481 gggaagtgtt ctttcaccat ctctgtcagt tgcaggttgg tttgcggatc gtcgatggcg
    33541 ttaatcagca cttccgcttt cgccgctccc gcagattcca gtaaatccat ccgcgtggca
    33601 tcgccataaa acactttcat accaaattta cgcaaggttt cgatatggtc cggatcgtga
    33661 tcgagcacca ccattttcac cccgctggag agcagtaaac gtccggtgat ctgcccaaaa
    33721 cgaccgaatc cggcaatgat tacccgcggc tgttcttcgt cgatctcatc ggcttcacgc
    33781 gcctcttcag tagaagattg ctcaaggcga ttgaggatca ccagcagaat cggcgttgcc
    33841 gccatcgaca gcgccaccgc cagggtcagc gatttcgccc actccggctc cagcacattc
    33901 gccatctgcg ccgcgccaaa taccacaaag gcaaactcac tgccctgccc taacaacacc
    33961 gcaaaccaac gacgctgttt atttggcact tgcaacggtc gggcaatcag ccacagcatg
    34021 gcgattttga tgatgaggaa accgagcagc aaaatgacaa tgcgcaatgg gttttcaagc
    34081 agcgtgccaa agtctatcga catgccaaca ccgatgaaaa acagccccaa cagcaaacct
    34141 ttaaagggtt cgatatcgct ctccagcgca tgacggtatt ccgagcttgc cagcagtacg
    34201 cccgccagaa acgcgcccat cgccatcgac aagccgacct cttccagcag caaaccaaag
    34261 ccaaacacga ggaataacgc cacggcacta aacacttccc gcaagccaga gcgggctaca
    34321 aaacgcagcg ccggacgcgt gacatagcgc cccagcaata ccaccagcac cagcgcaccc
    34381 gccactttta acgccgagag agcaaatgcg cccatcgtcg tcgaggcact gctcgttgcc
    34441 agtagcggaa tcatcgccac cagcgggatc gccgcgatat cctggaacag cagcaccgca
    34501 aaggcactgc gacccatttg cgtcaccatc agattgcgtt cattcatcgc ctgcatggca
    34561 atcgccgtag aggagagcgc cagcgtcatg ccgatcaatt ccgcgacctg ccagcgcagc
    34621 ccaagtaaca tgcagaacag ccccagcagg ccgccgcaaa tcaccatctg caatgcgcca
    34681 ccgccgaaca ctgccgcacg cagcttccac agcctttgtg gatcgagttc gaggccgata
    34741 ataaacagca tcagcaccac cccaatctcg gcaaagtgca gaatagattc ggcatcggtc
    34801 accagtcgca gcccccacgg gccaataatg cagccggcga tcaggtagcc aagtaccgat
    34861 cccagaccaa gacgtaccgc aatgggtaca atcagcgctg ccgaaccgag ataaatcagc
    34921 gcctgaatca gcgtatggct atccatgatg ggcctcctgc cattccagca gacgttgctt
    34981 atagtgacgc gcctgccctt cgagggtttc gtcgtcacaa ataaaggtgc agtgcatggc
    35041 aaacggtggc agccagttca gcccgcagta gattgccgtc gcctgtagcg gctgcgacag
    35101 cacatcaaag cccggatgcg caccaatttc aaaatggctt tccccgccgc cggtcgtcac
    35161 cgcccacagc aaatgtttgc catgcagcgc cttgccgccg tgaccgtaag cccagccgtg
    35221 cgacaaaact ttatcgatcc aaagtttaag aagcggagga atgctgtacc actgcatcgg
    35281 atgctgccag acgatcagat cggcgcgaga cagcgcctcc tgctcggcgg caatatcgat
    35341 attgaagtca ggatagagtt gataaagaga gcgaatttcg acgccttcca gcgtccttgc
    35401 ctgttcaagc atccgtttat tcgcatggga atgatgcgga tacggatgcg cataaattat
    35461 aagaatcata ggtagcccgc gtgattacgc ctttgttttc ccacaaagtg tagtcatata
    35521 ttcacgcggc ttatagtcaa taatattgat tcagaaaaga gagaaatctg aacaatttac
    35581 cccattctct gcccgcggat cgtcatattg ctggtttgcg ccaatgacat ccctcgatgt
    35641 ctccggggca aacgctacgg aaatcaacaa gccaaacagc gagatacccg cccccattag
    35701 catcgtgtta ctgataccgt aattattgat aaagatcggc agtgcccagg tcgaaacaat
    35761 ggtgccaata cggcttaagg acataatcac gcccacggca gaggcgcgga tatctgtcgg
    35821 gaagagttca ttaggataga gccactgcaa attacccggc ccgccagaga aaaaggcata
    35881 caccgcaaag gccatcacta ccagccagat ccccatatcc gggatcagcc ccaaaaccgc
    35941 cagctccagc gtcatcatgg caaagctgcc aatcaacaat ggacgccgtc cggcagtgtt
    36001 taaccacagc atcggcggaa tacagccgag cataaagaac aggctaatca ccacgttccc
    36061 cagcgccgcg tttttgccaa cacccaatcc caacaaacca acgatttgcg ggccaaaggt
    36121 gtaaatggcg aacattggga tcacctggca ggtccagatg gcggcaacaa acagaacaaa
    36181 aggaaaatgg cggcgattaa acagatcgcg aaaacgggtt tgctgcggct gctcttcatc
    36241 gaaagccacc ggttcgccaa acagttttat catcatctct tcgcactctt ttactcgccc
    36301 tttgcgtaat aaccagcggg gagattcagg cagttcgaat cgaccaatca aaatcaacaa
    36361 acaggggatc gccgcgctac ccagcatcca gcgccagccg ccttccacat cataaagcca
    36421 gtagccgacc agatcggcgc aggtcgcgcc gacataccac atggcggcga taaagctgat
    36481 ggaaaatgcc cgctgacggg tactggagaa ctcggtgatc attgaggtgg cgatgggata
    36541 atcggcaccg atgacaatgc cgataagtac ccgcatcacc aacagttcga cgggggatga
    36601 aacaaacatc gtcgccaccg atatcacgcc gatggcgatg atatcaatga ggaacatttt
    36661 gcgccgtccg actttatcgg aaatatagcc aaacagcgat gtgccaacga acagcccggc
    36721 gagcgttccc gcgcccaaca agccaatcca gtcagcgtcc agttttagcg ccggagtcag
    36781 ttgctccagc gccacgccaa tcattaccag tacataacca tcca
//
LOCUS       DAJTMC010000020        36533 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.020, whole genome shotgun sequence.
ACCESSION   DAJTMC010000020 DAJTMC010000000
VERSION     DAJTMC010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36533
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.020"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            <1..896
                     /locus_tag="OO130_000888"
     CDS             <1..896
                     /locus_tag="OO130_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5364395.1"
                     /translation="DIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEEQ
                     IGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNTY
                     PTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELENL
                     TISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGINP
                     TIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSIR
                     NHRYTATMLQHIAEDRDGTNH"
     gene            complement(893..2370)
                     /gene="scpC"
                     /locus_tag="OO130_000889"
                     /pseudo
     CDS             complement(893..2370)
                     /gene="scpC"
                     /locus_tag="OO130_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
     gene            complement(2394..3179)
                     /gene="scpB"
                     /locus_tag="OO130_000890"
     CDS             complement(2394..3179)
                     /gene="scpB"
                     /locus_tag="OO130_000890"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HCR5364396.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(3190..4185)
                     /gene="meaB"
                     /locus_tag="OO130_000891"
     CDS             complement(3190..4185)
                     /gene="meaB"
                     /locus_tag="OO130_000891"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HCR5364397.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPYCGNTLRLGVTGTPSAGKRTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKIRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLIVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRVLTCSALEKRGIDEIWHAIIEFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(4178..6322)
                     /gene="scpA"
                     /locus_tag="OO130_000892"
                     /pseudo
     CDS             complement(4178..6322)
                     /gene="scpA"
                     /locus_tag="OO130_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417392.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
     gene            complement(6526..7419)
                     /gene="argP"
                     /locus_tag="OO130_000893"
     CDS             complement(6526..7419)
                     /gene="argP"
                     /locus_tag="OO130_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HCR5364398.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            7802..8461
                     /gene="rpiA"
                     /locus_tag="OO130_000894"
     CDS             7802..8461
                     /gene="rpiA"
                     /locus_tag="OO130_000894"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HCR5364399.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            8716..9948
                     /gene="serA"
                     /locus_tag="OO130_000895"
     CDS             8716..9948
                     /gene="serA"
                     /locus_tag="OO130_000895"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HCR5364400.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            10177..10236
                     /gene="ibsC"
                     /locus_tag="OO130_000896"
     CDS             10177..10236
                     /gene="ibsC"
                     /locus_tag="OO130_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HCR5364401.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            10569..10628
                     /locus_tag="OO130_000897"
     CDS             10569..10628
                     /locus_tag="OO130_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCR5364402.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(10729..11331)
                     /locus_tag="OO130_000898"
     CDS             complement(10729..11331)
                     /locus_tag="OO130_000898"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HCR5364403.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(11352..11535)
                     /gene="ssrS"
                     /locus_tag="OO130_000899"
     ncRNA           complement(11352..11535)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="OO130_000899"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(11577..11906)
                     /gene="zapA"
                     /locus_tag="OO130_000900"
     CDS             complement(11577..11906)
                     /gene="zapA"
                     /locus_tag="OO130_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HCR5364404.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            12074..12652
                     /gene="ygfB"
                     /locus_tag="OO130_000901"
     CDS             12074..12652
                     /gene="ygfB"
                     /locus_tag="OO130_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HCR5364405.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            12678..14003
                     /gene="pepP"
                     /locus_tag="OO130_000902"
     CDS             12678..14003
                     /gene="pepP"
                     /locus_tag="OO130_000902"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HCR5364406.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIMNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     AEVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            14000..15178
                     /gene="ubiH"
                     /locus_tag="OO130_000903"
                     /gene_synonym="visB"
     CDS             14000..15178
                     /gene="ubiH"
                     /locus_tag="OO130_000903"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HCR5364407.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLESGETLTGRVLVA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTRAARA
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            15201..16403
                     /gene="ubiI"
                     /locus_tag="OO130_000904"
                     /gene_synonym="visC"
     CDS             15201..16403
                     /gene="ubiI"
                     /locus_tag="OO130_000904"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HCR5364408.1"
                     /translation="MQCVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADISLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLVLVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGTNPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(16477..16560)
                     /locus_tag="OO130_000905"
     CDS             complement(16477..16560)
                     /locus_tag="OO130_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5364409.1"
                     /translation="MSKNTKSKNNDIRKYNAKTEVKLVYFK"
     gene            16850..17944
                     /gene="gcvT"
                     /locus_tag="OO130_000906"
     CDS             16850..17944
                     /gene="gcvT"
                     /locus_tag="OO130_000906"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HCR5364410.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPADRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            17968..18357
                     /gene="gcvH"
                     /locus_tag="OO130_000907"
     CDS             17968..18357
                     /gene="gcvH"
                     /locus_tag="OO130_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HCR5364411.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            18476..21349
                     /gene="gcvP"
                     /locus_tag="OO130_000908"
     CDS             18476..21349
                     /gene="gcvP"
                     /locus_tag="OO130_000908"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000195053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HCR5364412.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLARAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETSISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYL"
     gene            21615..22358
                     /locus_tag="OO130_000909"
                     /pseudo
     CDS             21615..22358
                     /locus_tag="OO130_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417378.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(22415..23848)
                     /gene="bglA"
                     /locus_tag="OO130_000910"
     CDS             complement(22415..23848)
                     /gene="bglA"
                     /locus_tag="OO130_000910"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HCR5364413.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDSLFDELLKYNIEPVITLSHFEIPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHDNPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMINAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGEKL"
     gene            23893..24204
                     /locus_tag="OO130_000911"
     CDS             23893..24204
                     /locus_tag="OO130_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HCR5364414.1"
                     /translation="MQPNDITFSQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLTELIKVIADIYPGQTQFYVIEFKC
                     L"
     gene            24368..25027
                     /locus_tag="OO130_000912"
     CDS             24368..25027
                     /locus_tag="OO130_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HCR5364415.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSMCHFLAIYLYIGQ
                     A"
     gene            complement(25223..26203)
                     /gene="ygfZ"
                     /locus_tag="OO130_000913"
     CDS             complement(25223..26203)
                     /gene="ygfZ"
                     /locus_tag="OO130_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HCR5364416.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMTENQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAADDERVLLGVAGFQARAALANLFSELPSREKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            26446..26712
                     /gene="sdhE"
                     /locus_tag="OO130_000914"
     CDS             26446..26712
                     /gene="sdhE"
                     /locus_tag="OO130_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HCR5364417.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            26693..27100
                     /locus_tag="OO130_000915"
     CDS             26693..27100
                     /locus_tag="OO130_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin CptA"
                     /protein_id="HCR5364418.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(27140..27661)
                     /gene="fldB"
                     /locus_tag="OO130_000916"
     CDS             complement(27140..27661)
                     /gene="fldB"
                     /locus_tag="OO130_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HCR5364419.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            27773..28669
                     /gene="xerD"
                     /locus_tag="OO130_000917"
     CDS             27773..28669
                     /gene="xerD"
                     /locus_tag="OO130_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HCR5364420.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            28694..29404
                     /gene="dsbC"
                     /locus_tag="OO130_000918"
     CDS             28694..29404
                     /gene="dsbC"
                     /locus_tag="OO130_000918"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000715205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HCR5364421.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGAAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLESQAEQQMKAIW
                     CAKDKNKAFDDVMAGKAATPASCDIDIADHYALGVQLGVSGTPAIVLSNGTLVPGYQP
                     PKEMKEFLDEHQKMTSGN"
     gene            29410..31143
                     /gene="recJ"
                     /locus_tag="OO130_000919"
     CDS             29410..31143
                     /gene="recJ"
                     /locus_tag="OO130_000919"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HCR5364422.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGETLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQIEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            31234..32332
                     /gene="prfB"
                     /locus_tag="OO130_000920"
     CDS             join(31234..31308,31310..32332)
                     /gene="prfB"
                     /locus_tag="OO130_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HCR5364423.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            32342..33859
                     /gene="lysS"
                     /locus_tag="OO130_000921"
     CDS             32342..33859
                     /gene="lysS"
                     /locus_tag="OO130_000921"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HCR5364424.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(33902..34450)
                     /gene="idi"
                     /locus_tag="OO130_000922"
     CDS             complement(33902..34450)
                     /gene="idi"
                     /locus_tag="OO130_000922"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HCR5364425.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESNEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPNGIVENEVCPVFAARTNSALQINDDEVMDYQWCDLADVLHGIDATPWAFSP
                     WMVMQAANSEARKLLSAFAQHN"
     gene            34505..34576
                     /locus_tag="OO130_000923"
     CDS             34505..34576
                     /locus_tag="OO130_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5364426.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            complement(34573..34698)
                     /locus_tag="OO130_000924"
     CDS             complement(34573..34698)
                     /locus_tag="OO130_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5364427.1"
                     /translation="MNLLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(34700..36148)
                     /gene="uacT"
                     /locus_tag="OO130_000925"
     CDS             complement(34700..36148)
                     /gene="uacT"
                     /locus_tag="OO130_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HCR5364428.1"
                     /translation="MSAIDSQLLSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKEAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSVGL
                     SIIQVGIDWAAGGKGNPQYGNPVYSGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDALWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVTRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
BASE COUNT         8797 a         9197 c         9768 g         8771 t
ORIGIN      
        1 tggacatttt tatttcgaaa aagatgcgca atttcatttt attagcgcaa accaataata
       61 ttgcccgggc ggcagaaaaa atccatatga cggcttcgcc atttggtaaa agcattgccg
      121 cgctggaaga acaaattggc tatacgctat ttacccgcaa agataataat atcagcctca
      181 ataaagccgg tcaggaactg tatcaaaaac tgttcccggt ttatcaacga ctttctgcga
      241 ttgataatga aatccataac tcagggcgtc gttcacgaga aattgtgatt ggcatagata
      301 atacttatcc gacaattatt ttcgatcagt tgattagtct cggagataag tatgaaggcg
      361 tgaccgccca gccagttgag ttcagcgaaa acggcgttat cgataatctt tttgaccgtc
      421 agttagattt tattatctcc ccacagcatg tatctgcacg agttcaggaa ctggaaaacc
      481 tgactatcag tgaattacca ccgctgcgat tgggctttct cgtttctcgc cgttatgaag
      541 agcgtcaaga gcaagagctg cttcaggagc tgccctggct acaaatgcgc tttcaaaacc
      601 gggctaactt cgaagcaatg attgatgcga atatgcgacc ttgtgggatc aatcccacca
      661 ttatttatcg tccctacagt tttatggcca aaatcagcgc cgtggagcgc ggacattttc
      721 tgacggtgat cccgcatttt gcctggcgac tggtgaatcc ggcaacgctg aaatattttg
      781 atgcgccgca caggccgatg tatatgcagg aatatcttta ttcaatcaga aatcatcgtt
      841 ataccgccac gatgcttcag catattgctg aagatcgtga cgggacaaac cattaaccca
      901 gcatcgagcc ggttgcaatt aaattacggt gtaagtcgaa gacgtggcta agatcgtggt
      961 gaatatgtcc gccgggcgca ttttccagat agcgatgcag ataatcccga tacataggat
     1021 gtgcacaatt atcaatgata gtgcgggcgc gttgaagcgg ggaaagaccg cgcagatcgg
     1081 cgatcccttg ttcggtgatg atcactttga cgctgtgttc gctgtgatca acatggctgc
     1141 acattggcac gacggttgag atcttgcctt ctttagcaat cgacggagcc ataaagatcg
     1201 acagatacgc gttgcgttca aaatcaccgc tgccgccgat gccgttcatc agatcgaccc
     1261 cggctacgtg cgttgaattt gcatgcccgt aaatatcaaa ctccaggccg acgttcagag
     1321 cgatgacgcc cagacgacgg atgatttccg ggttattgga aatctcctgc ggacgcaaca
     1381 caatgcggct ggcaaagtaa tccatattgt cgtaaatctt gcgcagggaa tcggccgaga
     1441 ttgtcaggct ggaggcgctg gccccgctga ttttgtcggt ttccagtaaa tgcaccaccg
     1501 attcctgtag cacttccgaa tacatcatga acggaggaat ttccgggttt tcccccagac
     1561 gcgccattac cgcattattg atattgccca cgccactttg cagcggcaga aattccggcg
     1621 gaatacgcca tgcgccattt cctgcaataa gaacgtgacc acgttatcgg caatctgctg
     1681 gcacatggga ttttgcttat ccagcatatt accggcgtcg ggcaagttgg tttccacgac
     1741 ggcgacaatc tttttcggat cgatttgcac atagcgggta ccgacgcgat ccattgcatg
     1801 gaagatcgac acgctattgc gccgcggtgg cgcgccagga atcacaatat ctgccagttc
     1861 tgcaacgcgc ggatcgtgat agtgattaag ttcaatgatc actttcttcg ctcgcagcag
     1921 ccaggtcggc gcattaccga tcccgctggt taaccagact cgaccatccg gtgccagtgc
     1981 cgatgcttca atgacggcaa catcaatgtc gccgaagaaa ccgtaattga ccatttgcgc
     2041 cacttcgctc aaatgcaggt caacgaaact caccgcgccc tgattgatct ttttacgtaa
     2101 accggacgat gtttgatatg gcgcacgcca ggaaacagca tcggcgtcag aaagtacatc
     2161 gtcagcggcg gcgctgattg acgcccccgt cagcaggcga atttgatacg gctttttggc
     2221 ctcatgctgt tcgttagctc tgcgggcaat cgcggtgggt agggctttcg gcgaacccgc
     2281 cggggtaaag ccgctaaatg ccaccatgtc gttatgctgg ataatttctg ccgcttcatc
     2341 ggcggtcatc cttgtccact gagtttccat ttttactcct tcgttcgcag ggattaatga
     2401 ccaacgaaat taggtttacg tttttcgagg aaagcgttca ttccttcctg gtaatcttcg
     2461 ctgtcataaa ccgcgcgtcg catcccctga atacgttcaa attcatcgga gttcatggtg
     2521 tgcgcttcgc ccagtacacg cagctcttct ttgataacag caatggctaa cggcgctttc
     2581 tcagagatgt ggtgcgccat ttgtaaggtg aaatcttcca gttcttccac ttccacaaca
     2641 tggttgagga tgccgacagc cagcgcgcgc tgggcggtga ttggcgaagc ggtaaaaatc
     2701 agctctttga caatgtggaa gcccgcgtcg cgggtcaggt tgtgaatgcc gaccaggtta
     2761 tacgggacgc cgaggtttac aggcgtcatt gagaaggttg aggtactggc ggcgatgatc
     2821 agatcggaac tcatgatcat ttcaaatgcg ccaccccaaa cactaccttc caccatcgaa
     2881 atgatcggtt tcgggaattt ttggatcatg cgggtgattt gacgcaatgg atcatcatag
     2941 gagagcggat cgcgaccgcc agacggcagt tcgtgaatat cgtgacctgc ggagaagact
     3001 ttggatccac tcggcgcgcg caaaatgata cagcgaattt ccggccggtt gagatcgctt
     3061 aacgcctgca taagatcatc aataaagact ttacttaagg cattaagttt tcggccatag
     3121 ttaaactcaa tgaccgccac tttgttgata gtggcaacgt tcacatactg ataagacata
     3181 aaaattcctt taatcaaaat attgcgtctg gataaattca ctgagctgcc gcaggccggt
     3241 gcgcggtgag agcgtattgt ttttgaccgc taaaagcgtc tggcggtaat agcgatcgaa
     3301 atcttcattc gcgaacaggt gattcagtac ttcttcttcg gtctgcttac gcagccattc
     3361 caccgattgt tgttgccgca cttgttgtaa acgaccactg gcagttagcg cggttttgaa
     3421 ctcgatgata gcgtgccaga tctcatcgat tccacgtttt tccagtgcgc tacaagtcag
     3481 aacccgtggc tgccattcgt cgtatttacg tcgcagaata tgcagggcac tctcgtacat
     3541 atgccgggca atggcgacat tggtatggtt atcgccatcg tctttgttga taacgatcag
     3601 atcagccact tccatcagcc cttttttaat gccctgcaga tcatcgccgc caccggcaat
     3661 ttgcaacgag ataaaacagt ccaccatgcg ggcgacttct gtttccgact gcccgacgcc
     3721 aaccgtttcg acaatcacta cgtcataacc cgccgcttcg cataacagca ttaattcccg
     3781 cgctcgctga ctggcaccgc ccagatgacc ggaggatggt accgggcgaa taaacgccgc
     3841 ttcggcacgc gccaggtcat tcatgcggat tttatccccg agaatgctac cgccagtgac
     3901 cgggctgctg ggatcgaccg caataaccgc gacctttaat ccctctcgaa tcaacaacat
     3961 gccaaaggcc tcaagaaagg ttcttttccc cgcgctgggg gtgccggtaa cgcccagtcg
     4021 cagggtgtta ccgcagtacg gcataatggc atcaagcagc tgcgtactta gtgcctgatg
     4081 acgcgggtga cggctttcca ccagcgtcat ggcctgggcg agtgtggcac gctcaccctg
     4141 acgtaagcgg cgaatacttt ctgccagcgt ggcttcatta atcatgatgc tggcttatca
     4201 gattcagtac gtcgcgcaca ctgtcgagca taggtgtacc tggaccataa atcgccgcca
     4261 cgccgcgctc ttgcaggaag gcgtaatcct gcggtggaat gacgccaccg gcgactacgc
     4321 agatatcttc gcgtccccat tttttcagcg cctcgaccag ttccgggatc agcgttttat
     4381 gaccggcagc cagtgaggat gcgcccacta cgtgaacgtc attttctact gccaggcggg
     4441 cgatctcttc aggtgtagag aacatcgggc ttaaatctac gtcgaaaccg agatcggaat
     4501 aggcgctggc gatcactttc gagccacgat cgtgcccatc ctggcccatt ttggcgatca
     4561 gaatgcgcag gcgacgacca ttgtcggcaa ggaactgctc cgtttgcgca acaatggcat
     4621 cgaactcgga ggccgatttc tcagattgat gatagctttg cgcaatcacg ccggtaacac
     4681 actggcttgg caccagataa cggtcgaaag cggcttccag cgcatcggaa atttcaccca
     4741 gggtggcgcg aacgcgagcg gcattaacag cggcagccag caggttttcg ttatgctgtg
     4801 cggcgtgagt cagggcgttc aacgcggcgg ttacggcggc atcatcacgg gtggcgcgaa
     4861 tgcgttccag cgaagcaatt tgctcgttac gcaccatcac gttgtcgatc tcaagtacat
     4921 cggtttcgtc ttcgtgatcc agtttgtact tgttgacacc aacgatgaca cgtttgccct
     4981 ggtcgataag cgactgttcg cgcgctgagg cctattcgat cattcgtttt ggcagacctg
     5041 cttcgatcgc tttcgccata ccaccggctt cgtcgatctg ttggataata gctctggctt
     5101 gtttgacgat ttgatcggtc agcgactcaa tgtaatagga tccggccagt ggatcgacgg
     5161 tgcggcagag ttctgattct tcctggatga tgatctgggt gttgcgggca atgcgtgctg
     5221 agaaatcggt aggcaaacca agcgcttcgt caaaggcgtt ggtatgcagt gactgagtac
     5281 cgcccagcgt cgcagccagt gcttcaatgg tggtgcggat aacgttgtta tacggatcct
     5341 gttcagtcag gctccagcct gaggtctggc agtgggtacg cagcgccagt gatttcgggt
     5401 cctgtgcgcc aaatccactg accgcttcgc tccataaata acgtgccgca cgcaacatgg
     5461 cgacgttcat aaacagatcc atgccgatgc cgaagaagaa cgacaggcga ggagcgaagt
     5521 catcaatttt cagtccggca gagattgctg ctttgatgta ctcaatccca tcagcgagcg
     5581 taaatgctac ctgctgcacg cagttggcac ccgcttcacc catgtggtaa ccgctgatac
     5641 tgatggtatt aaatcgcggc atgttgccgg aacaccaggc gatgatgtcg gcgataatgc
     5701 gcattgacgg ttttggcggg taaatatagg tgttgcggca gaggtactct ttgaggatat
     5761 cgttctgaat ggtgccggtc agtttatcag gtgtaacacc ttgctcttcc gcggcgacga
     5821 tataaaacgc cagtactggt agcactgcgc cattcatggt catcgaaacc gacattttat
     5881 ccagcgggat ctggtcgaac aggactttca tatcttccac ggtgtcgata gcgacgcccg
     5941 ctttgccgac gtcgcccgcc acgcgcgggt tatcggagtc gtagcctcgg tgggtggcaa
     6001 ggtcaaacgc aacggaaaga cctttttgcc cggcggccag gttacggcga taaaaagcgt
     6061 tggactcttt tgctgttgaa aaaccagcat actgacggat ggtccacggt tgggcggtat
     6121 acatagtggc acgcgggcca cgaacgtagg gcggcaaacc aggaagggta cctgtcacct
     6181 ccagattatc gagatcggct tcggtataca gcggcttgat ggtgatccct tccgcggttt
     6241 gctgaaccag cgagtcgaca gttttctccc gacggctcaa ttccttgttg gcaagctgtt
     6301 gccactcctg cacgttagac attatttgct ccgtaaaacg caatcaaaat gagggtattt
     6361 gcgcctacgg tgagcttttt acgtgggttt gtcgccagca aaaaaaatgc attcagacgt
     6421 cgttttttgc cgcctgagat ttgtctattt tgtgatgtga ttcaaggaat acaaaaagaa
     6481 gtgaggacaa cgcctgatat gtgctatcag gcattatttg atggattaat cctgacgaag
     6541 gactttgtga ccataatcga gcaacgcatc agtgacttta cgcatcatgc ggctttcagg
     6601 agcaaagcgg tgccagtaga gcatccggcg ttgaaatagc cctggcgtta agtcaatcag
     6661 ttcaccgctg gctagttctt tctcgatttg caggtgcggg atcatacagc aggtggtgcc
     6721 ctggcgagca agttgcacga acgcttctga agaattaacg atatggcagg gcacgctgcc
     6781 tggaggcaga tcgaagtttt gctgcaaaaa ggcctggtgc atatcgtcaa gatggtcaaa
     6841 ggcgaccaca ggtgctttca ataatgccga acgcgttacg ccgttaggga aatatttttc
     6901 ggcaaacggt tttgagctga cgaacagata gtcaagcgca ccgagcttat cgacaaggca
     6961 actcggcagc gcctgatgtt gaatactcac cgcgccgacc acttcgccgc ggcgcagacg
     7021 ttcctgagta cgggtttcat cttctacctg caagttgagg cggataggcg aatcagccaa
     7081 tacaggagcc agtgccggaa gcaaccacgt cgccagactg tcggcgttga ccgccagtga
     7141 aagcagcagc ggagtcgaac cggtttgttc atcgcccagc cactcttctt ccagcaactc
     7201 cacctggcgc agcagtgcca gcagtttttg cccttgttcc gtcgggcgcg gcggtacggt
     7261 acgcaccaac agcggctgcc cgaacatatt ttccagttgc ttaatgcgct gtgagacggc
     7321 tgattgtgta atgcacagct tttgtgccgc gcgctcaaat cctcgttcac gtatcaccgc
     7381 atccagtgcc tgtaatgttc tgtagtccgg gcgtttcatt gctgttgtct gctcctggta
     7441 aatgcttttt cctgcactat gacacaattt tgtgtcaggt tgcatatacc gcagccgtcg
     7501 gcgggtaaca tttgtatcaa tggttaaacg cgcagcttaa aagatcaggg atttgcgcta
     7561 ttgcatttcg cttagcgact tcgcgccctg gttatgttga atggcgtggc gttattgcct
     7621 ctgttcgcac cgtggagcgg catacccaga ccgttgtatg ctttatttgc ggggcgaaag
     7681 gggatgcccg ccattgcgcg atattttatg gatgagttaa ccacccggct tgccaacggg
     7741 gtctgaatcg ctttttttgt atataatgcg tgtgaaattt cataccacag gcgaaacgat
     7801 catgacgcag gatgaattga aaaaagcagt aggatgggcg gcacttcagt atgttcagcc
     7861 cggcaccatt gttggtgtag gtacaggttc caccgccgca cactttattg acgcgctcgg
     7921 cacgatgaaa ggccagattg aaggggccgt ttccagttca gatgcttcca ctgaaaaact
     7981 gaaaagcctc ggcattcacg tttttgatct caacgaagtc gacagccttg gcatctacgt
     8041 tgatggcgca gatgaaatca acggccacat gcaaatgatc aaaggcggcg gcgcggcgct
     8101 gacccgtgaa aaaatcattg cttcggttgc ggaaaaattt atctgtattg ccgacgcctc
     8161 caagcaggtt gatattctgg gtaaattccc gctgccagta gaagttatcc cgatggcacg
     8221 tagtgcagtg gcgcgccagc tggtgaaact gggcggtcgt ccggaatacc gtcagggcgt
     8281 ggtgaccgat aatggcaacg tgatcctcga cgtccacggc atggaaatcc ttgacccgat
     8341 agcgatggaa aacgccataa atgcgattcc tggcgtggtg actgttggct tgtttgctaa
     8401 ccgtggcgcg gacgttgcgc tgattggcac acctgacggt gtcaaaacca ttgtgaaatg
     8461 atctgacggg ggaacctccc ccgttaaaaa aattctcttc attaaatttg gtgacatgtg
     8521 tcacgttttt acctggctat tgtcgattgc tctaaataaa tcctctaaac cagcatattc
     8581 aaccaagaaa tacctttgcg tgatatttcc tcaacatcgc gacgcaaacg ttcatattgc
     8641 cgcaatatta ttttttgata tgttgaaagg cggatgcaaa tccgcacaca acatttcaaa
     8701 gacaggattg ggtaaatggc aaaggtatcg ctggagaaag acaagattaa gtttctgctg
     8761 gtagaaggcg tgcaccaaaa ggcgctggaa agccttcgtg cagctggtta caccaacatc
     8821 gaatttcaca aaggcgcgct ggatgatgaa caattaaaag aatccatccg cgatgcccac
     8881 ttcatcggcc tgcgatcccg tacccatctg actgaagacg tgatcaacgc cgcagaaaag
     8941 ctggtcgcta ttggctgttt ctgtatagga acaaaccagg ttgatctgga tgcggcggca
     9001 aagcgcggga tcccggtatt taacgcaccg ttctcaaata cgcgctctgt tgcggagctg
     9061 gtgattggcg aactgctgct gctattgcgc ggcgtgccgg aagccaatgc taaagcgcac
     9121 cgtggcgtgt ggaacaaact ggcggcgggt tcttttgaag cgcgcggcaa aaagctgggt
     9181 atcatcggct acggtcatat tggtacgcaa ttgggcattc tggctgaatc gctgggaatg
     9241 tatgtttact tttatgatat tgaaaataaa ctgccgctgg gcaacgccac tcaggtacag
     9301 catctttctg acctgctgaa tatgagcgat gtggtgagtc tgcatgtacc agagaatccg
     9361 tccaccaaaa atatgatggg cgcgaaagaa atttcactaa tgaagcccgg ctcgctgctg
     9421 attaatgctt cgcgcggtac tgtggtggat attccggcgc tgtgtgatgc gctggcgagc
     9481 aaacatctgg cgggggcggc aatcgacgta ttcccgacgg aaccggcgac caatagcgat
     9541 ccatttacct ctccactgtg tgaattcgac aacgtacttc tgacgccaca cattggcggt
     9601 tcgactcagg aagcgcagga gaatatcggc ctggaagttg cgggtaaatt gatcaagtat
     9661 tctgacaatg gctcaacgct ctctgcggtg aacttcccgg aagtctcgct gccactgcat
     9721 ggtggtcgtc gtctgatgca catccacgaa aaccgtccgg gcgtgctaac tgcgctgaac
     9781 aaaatcttcg ccgagcaggg cgtcaacatc gccgcgcaat atctacaaac ttccgcccag
     9841 atgggttatg tagttattga tattgaagct gacgaagacg ttgccgaaaa agcgctgcag
     9901 gcaatgaaag ctattccggg taccattcgc gcccgtctgc tgtactaatt ccccttctct
     9961 gaaaattaac gggcaggtca ctgacttgcc cgttttttta tcccttctcc acaccgttgc
    10021 cgcgttgcgc attctacttt gcgagtctgc tccagaaaat aaatcaaagt cagatcaatg
    10081 cgttgtgttt atttaatggg gatttacgat ggcgggacag tatggggctg taacggtaaa
    10141 gccctcaccg aagcgagggc ttaaaggaga agggttatga tgcgacttgt catcatactg
    10201 attgtactgt tactgataag tttcggagct tattaacagt cagtctctgg ggaggagtca
    10261 tcctccctta cccttactca ctaaattagg tcaaagaatc aacgatgtca atcagggcga
    10321 tgcggggtgc atcgccctga accgaacttt ttgatagcca cttcatcgcc cacgtttact
    10381 gccgtaatag atcctttttc ccaaaaatta tctctgtttt gcatggggtt tttcgaacac
    10441 gctcgaagaa aagatttaaa ggtagatcaa tgcgttgtgg ttatttaaag agagcttacg
    10501 atagctgggc agcatggggc tgtaacggta aagccctcac cgaagcgagg gcttgaagga
    10561 gaagggttat gatgcgactt gccatcatac tgattgtact gttactcata agtttcccag
    10621 cttattaaca gtcagtcaca ggggaggagc aatcctccct tacccttact cactaaatta
    10681 ggttaaagaa tcaacgatgt caatcagggc gatgcaggtg tatcgccctt accactccca
    10741 gactttcgac ggtgtaacca ccgcaggaag agggatatcc cactcttcaa cggggagttt
    10801 ttccaccaac tgacaatcat gcgcataacc caccggttgc gttttatagt gctgccagtt
    10861 ttgtaaggtc cgatcataaa aaccaccgcc cattcccagg cgctgaccgt actcatcaaa
    10921 ggcgaccagc ggtgtgatca gcacgtctaa tcgggaaagg ggtagcacgt cacgcacatc
    10981 cagttttggc tcatggattt tcaacctgtt catcaccagt tcgctttgcg gatggtaatt
    11041 caggaacagc aaattaccag cactaaaggg atgcaaaact ggaagatata cgcgcttacc
    11101 ggcgcgccag agttgttcta ttagcggctg ggtgtcgagt tcaccatcaa aagagaggaa
    11161 tacagcgacc gtatgcgcca tcaccaccgg gggataagtc atcatccggg tagcggcttg
    11221 ttgacccatt tcctgctgtt gttccggcgt taacgcacga cgacgttgcc gaatcatttt
    11281 gcggatttct tgtcgggata atgtcagtgg gagttctggt agttgcgtca tggctggtgc
    11341 cagataagaa gggaatctcc gagatgccgc cgcaggctgt aacccttgaa cccttggttc
    11401 aaggtgaatg tgtcgtcgca gttttaaggc ttctcggacg gaccgagcat gctcaccaac
    11461 cgcggagcgc cacattcttg tggtatgaaa tatcggctca ggggactggc ccgcttgcga
    11521 acatctcaga gaaattttgt cttcacggtt actctaccac agtaaaccga aaagtgtcat
    11581 tcaaagtttt ggttagtttt ttcggtgatg cgaccttgtt caagtaacgc ttgttctatg
    11641 gtctgctgca gcatccgaat acgctgttcc atacttgccg cgtagtcacg agtcttcgct
    11701 ttttcttgcg ctaactcata gctgatattc aatgcggcaa tgaagaccaa ctgttcagta
    11761 tttgtgactc tagtgcgttc tttcagatct tgcaaccgtt ggttcagatc gtccgctgcc
    11821 tgattcaacg catccctttg gtcaggcggg cagttcacac gcagtgaacg gccaaaaatt
    11881 tggatatcga cgggttgtgc agacatgcca ccttcctgct gattgactgc gctgccttcg
    11941 tctttagacc catggtctgc gaaggggcga cactatagct accctgatga gaagagacaa
    12001 gcccttttct ggtccaccag ggcccaaagt ggtagcatat catgaatatt cctccctttg
    12061 acgacgaatg cttatgtcta tacagaacga aatgcctggt tacaacgaaa tgaaccagta
    12121 tctgaaccaa caagggacgg gtctgacccc tgccgagatg catggtttaa tcagtgggat
    12181 gatatgtggc ggtaacgatg acagctcatg gctgccgcta cttcacgacc tgacgaacga
    12241 aggcatggct ttcggtcatg agctggcaca ggcactgcgt aaaatgcact ctgccaccag
    12301 cgatgccctg caggatgacg gcttcctttt tcagctttat ctgcctgatg gcgatgatgt
    12361 cagcgttttc gatcgggctg atgcattggc aggttgggtc aatcacttcc tgcttggtct
    12421 tggcgttacg caaccgaagc tggataaagt gaccggcgaa accggtgaag ctatcgacga
    12481 tctgcgtaac attgcgcaac tgggttacga cgaagacgaa gatcaggaag agcttgaaat
    12541 gtcgcttgaa gagatcatcg aatacgttcg tgttgccgcg ctgttatgcc acgacacctt
    12601 tactcatccg caaccgaccg cgccagaagt acaaaaaccg actctacact aaaaacaaaa
    12661 aacgtaagga gagtgttatg agtgagatat cccggcaaga gtttcagcgt cgccgtcagg
    12721 ccctggtgga gcaaatgcaa cccggcagcg ccgcgctgat ttttgctgca ccagaagtaa
    12781 cacgtagcgc cgacagcgaa tacccctatc gtcagaacag tgacttctgg tacttcaccg
    12841 gctttaacga accggaagcg gtgctggtgc tgattaaaag cgatgacact cataaccaca
    12901 gcgttctgtt taaccgcgtt cgcgacctga cggcggagat ctggtttggc cgtcgcttag
    12961 gccaggatgc cgcgccagag aaactgggcg ttgaccgcgc actggcattc agcgaaatca
    13021 atcagcaact ttatcaacta cttaacggcc tggatgtggt ttaccatgcc cagggcgaat
    13081 atgcatatgc tgatgaaatc atgaacagtg cgctggaaaa actgcgtaaa ggttcgcggc
    13141 aaaatctcac cgcaccggca acgatgatcg actggcgtcc tgttgttcat gaaatgcgcc
    13201 tgttcaaatc gccagaagag attgccgtac tccgccgcgc gggagaaatc accgccatgg
    13261 cacatacacg ggcgatggaa aaatgccgtc cgggaatgtt cgagtaccat ctggaaggcg
    13321 aaattcacca cgaatttaac cgccacggtg cgcgctatcc gtcctataac accattgtcg
    13381 gcagcggtga aaacggctgc attctgcact acaccgaaaa cgagagtgaa ctgcgcgacg
    13441 gtgacctggt gttgattgac gcgggttgtg aatacaaagg ttacgcgggc gatattaccc
    13501 gcaccttccc ggtcaacggc aaattcaccc aggcccagcg tgaaatctac gacattgtgc
    13561 tggagtctct cgaaaccagc ctgcgcctgt atcgtccggg aacttccatt ctggaagtca
    13621 ctggtgaagt ggtgcgcatc atggttagcg gcctggtaaa actcggcatt ctgaaaggtg
    13681 atgttgatga actgatcgct cagaatgccc atcgtccttt ctttatgcat ggccttagcc
    13741 actggttagg gctagatgtc catgacgttg gcgtttatgg tcaggatcgc tcgcgcattc
    13801 tggaaccggg catggtactg accgtagagc cagggctgta tattgcgccg gatgcagaag
    13861 tgccagaaca atatcgcggt atcggcattc gtattgaaga cgacattgtg attaccgaaa
    13921 ccggtaacga aaacctcacc gccagcgtgg tgaaaaagcc ggaagaaatc gaagcgttga
    13981 tggctgctgc gagaaagcaa tgagcgtaat catcgtcggt ggcggcatgg cgggcgcgac
    14041 gctggcgctg gctatttccc ggttaagtca cggggcgctg ccggtacatt tgattgaagc
    14101 gactgcgcca gagtcacatg ctcatccggg ctttgatgga cgagcgatag cgctggcggc
    14161 gggtacctgt cagcaactgg cgcgcatcgg cgtctggcaa tctctggcgg attgcgcaac
    14221 tgccatcacc accgtgcatg tcagcgatcg tggtcacgct ggatttgtca ccctcgccgc
    14281 agaagattac caactggcgg cgctgggaca ggttgtcgaa ttgcacaatg tcgggcaacg
    14341 gctgtttgca ttgctgcgta aagcacctgg cgtaacgctg cattgccctg atcgcgtggc
    14401 taacgttgcc cgtactcaga gtcacgttga agtgacgctg gagagtggcg agacgctgac
    14461 gggccgcgtg ctggtagcag ctgatggcac ccattcagcg ttagccaccg cgtgcggcgt
    14521 tgactggcag caggagcctt acgaacaact ggccgtgatt gccaacgttg cgacttccgt
    14581 tgcgcacgaa ggccgcgctt ttgaacgctt cacgcaacat ggcccgctgg cgatgttgcc
    14641 gatgtctgac ggacgctgtt cgctggtctg gtgtcatcca ctggaacggc gcgaagaggt
    14701 gctgtcgtgg agcgacgaga agttttgccg tgaactccag tcggcctttg gctggcgact
    14761 tggaaaaatt acccacgccg gtaaacgcag tgcttatccg ctggcgttaa cccgcgccgc
    14821 cagagctatt acccatcgta ccgtgctggt gggcaatgcg gcgcaaactc tgcacccgat
    14881 tgccgggcaa gggtttaacc tcggtatgcg agatgtcatg agccttgcgg aaaccctgac
    14941 tcaggcgcat gagcgcggag aagacatcgg tgattacggc atattgtgcc gctatcagca
    15001 gcgtcgacag agcgatcgcg aagcaaccat cggcgtcacg gacagcctgg tacatctttt
    15061 tgccaaccgt tgggcaccgc tggttgtcgg gcgcaacatc ggactgatga cgatggaatt
    15121 attcaccccg gcacgcgatg tgctggcgca gcgcaccctc ggttgggtgg cgcgttgaag
    15181 gcggttttaa ggaataaaca atgcaatgtg ttgatgtagc cattgttggt ggcggcatgg
    15241 tggggctggc ggttgcctgc ggcttacagg ggagcggctt acgcgttgcc gtactggagc
    15301 agcgcgtacc ggaacctctg gcggcggatg caccaccaca actgcgcgtt tcggctatca
    15361 atgccgccag cgaaaaatta ctcacccgtc ttggcgtctg gcaggacatt ctctctcgta
    15421 gggccagttg ttatcacggt atggaagtgt gggacaaaga cagttttggt cacatttcgt
    15481 ttgacgatca aagcatgggc tatagccatc ttgggcatat cgttgaaaac tcagtgattc
    15541 actacgcgct gtggaacaaa gcgcagcagt cgtcagatat cactctgttg gcccccgcag
    15601 aattacagca ggtcgcctgg ggagaaaatg aaactttcct gacgctgaaa gatggcagca
    15661 tgttaacggc gcgtctggtg attggcgcgg acggtgctaa ttcctggttg cgcaacaaag
    15721 cagatatttc gctgactttc tgggattatc agcatcacgc gctggtagcg accattcgca
    15781 cggaagaacc gcatgatgcg gtggcgcggc aggttttcca tggcgaaggc attctggcct
    15841 ttttaccgct tagcgatccg catctttgct caattgtctg gtcactgtcg ccagaggaag
    15901 cgcagcggat gcagcaggca agtgaagatg aatttaatcg cgcgttaaat atcgcttttg
    15961 ataatcgcct gggcttatgc aaggttgaga gcgcgcgtca ggtgttccca ctgacggggc
    16021 gttatgcgcg ccagtttgcc gcgcaccgtc tggtgctagt gggcgacgcc gcgcatacca
    16081 ttcacccgct ggcggggcag ggagtaaatc tcggctttat ggatgctgca gagctgattg
    16141 ctgaactgaa acggttgcat cgtcagggga aagacatcgg gcagtacatt tacctgcgcc
    16201 ggtatgagcg tagccgcaag cacagtgcgg cgctgatgct ggctggtatg cagggattcc
    16261 gcgatctgtt ttccggtacc aatccggcga aaaaactgct gcgtgatatt ggtttgaaac
    16321 tggccgatac gcttcctggc gttaaaccac aacttatccg ccaggcaatg ggattaaacg
    16381 atttgcctga atggctgcgt taaaaatttc tcttctgctg tttatttgat acccatcaca
    16441 ctttcatctc ccggtttttt cgccgggaga ttttcctcat ttgaaataaa ctaatttcac
    16501 ctccgttttc gcattatatt ttctaatgtc attatttttt gatttagtgt tttttgacat
    16561 ttttttagct cttaatattg ccttattcaa attagatttc taatggcatc atctttatat
    16621 agaaactggt gtgttttttg gttttttatt ctgtcgcgat tttggcgttt tttaaccata
    16681 agctaatgtg atgatcaatt ttaccttatg gttaacagtc tgtttcggtg gtaagttcag
    16741 gcaaaagaga acgattgcgt tggggaccgg gagtggctcc gatgctgggt ttcgtggtga
    16801 taatttcacc atgaaaagtt gtcagccccg cttattcaat gaggacaaga tggcacaaca
    16861 gactcctttg tacgaacaac acacgctttg cggcgctcgc atggtggatt tccacggctg
    16921 gatgatgccg ctgcattacg gttcgcaaat cgacgaacat catgcggtac gtaccgatgc
    16981 cggaatgttt gatgtgtcac atatgaccat cgtcgatctc cgcggcagcc gcacccggga
    17041 gtttctgcgt tatctgctgg cgaacgatgt ggcgaagctc accaaaagcg gcaaagccct
    17101 ttactcgggg atgttgaatg cctctggcgg tgtgatagat gacctcatcg tctactactt
    17161 tactgaagat ttcttccgcc tcgttgttaa ctccgccacc cgcgaaaaag acctctcctg
    17221 gattacccaa cacgctgaac ctttcggcat cgaaattacc gttcgtgatg acctttccat
    17281 gattgccgtg caagggccga atgcgcaggc aaaagctgcc acactgttta atgacgccca
    17341 gcgtcaggcg gtggaaggga tgaaaccgtt ctttggcgtg caggcgggcg atctgtttat
    17401 tgccaccact ggttataccg gtgaagcggg ctatgaaatt gcgctgccca atgaaaaagc
    17461 ggccgatttc tggcgtgcgc tggtggaagc gggtgttaag ccatgtggct tgggcgcgcg
    17521 tgacacgctg cgtctggaag cgggcatgaa tctttatggt caggagatgg acgaaactat
    17581 ttctccttta gccgccaaca tgggctggac tatcgcctgg gaaccggcag atcgtgactt
    17641 tatcggtcgt gaagccctgg aagtgcagcg tgagcatggt acagaaaaac tggttggtct
    17701 ggtgatgacc gaaaaaggcg tgctgcgtaa tgaactgccg gtacgcttta ccgatgcgca
    17761 gggcaaccag catgaaggca ttatcaccag cggtactttc tccccgacgc tgggttacag
    17821 cattgcgctg gcgcgcgtgc cggaaggtat tggcgaaacg gcgattgtgc aaattcgcaa
    17881 ccgtgaaatg ccggttaaag tgacaaaacc tgtttttgtg cgtaacggca aagccgtcgc
    17941 gtgatttact tttttggaga ttgattgatg agcaacgtac cagcagaact gaaatacagc
    18001 aaagaacacg aatggctgcg taaagaagcc gacggcactt acaccgttgg tattaccgaa
    18061 catgctcagg agctgttagg cgatatggtg tttgttgacc tgccggaagt gggcgcaacg
    18121 gttagcgcgg gcgatgactg cgcggttgcc gaatcagtaa aagcggcgtc agacatttat
    18181 gcgccagtaa gcggtgaaat cgtggcggta aacgacgcac tgagcgattc tccggaactg
    18241 gtgaacagcg aaccgtatgc aggtggctgg atctttaaaa tcaaagccag cgatgaaagc
    18301 gaactggaat cactgctgga tgcgaccgca tacgaagcat tgttagaaga cgagtaacgg
    18361 ctttattcct cttctgcggg agaggatcag ggtgaggaaa atttatgcct caccctcact
    18421 ctcttcgtaa ggagagaggt tcacaattca ctgcacgttt caggaaccat cgctcatgac
    18481 acagacgtta agccagcttg aaaacagcgg cgcttttatt gaacgccata tcggaccgga
    18541 cgccgcgcaa cagcaagaaa tgctgaatgc cgttggcgca caatcgttaa acgcgctgac
    18601 cggccagatt gtaccgaaag atattcagct tgcgaccccg ccgcaggttg gcgcaccggc
    18661 gaccgaatac gccgcactgg cagaactcaa ggctattgcc agtcgcaata aacgcttcac
    18721 gtcttacatc ggcatgggtt acaccgccgt gcagctaccg ccggttatcc tgcgtaacat
    18781 gctggaaaat ccgggctggt ataccgcgta cactccgtat caacctgaag tctcccaggg
    18841 ccgccttgaa gcactgctca acttccagca ggtaacgctg gatttgactg gactggatat
    18901 ggcctctgct tctcttctgg acgaggccac cgctgccgcc gaagcaatgg cgatggcgaa
    18961 acgcgtcagc aaactgaaaa atgccaaccg cttcttcgtg gcttccgatg tgcatccgca
    19021 aacgctggat gtggtccgta ctcgtgccga aacctttggt tttgaagtga ttgtcgatga
    19081 cgcgcaaaaa gtgctcgacc atcaggacgt cttcggcgtg ctgttacagc aggtaggcac
    19141 taccggtgaa attcacgact acactgcgct tattagcgaa ctgaaatcac gcaaaattgt
    19201 ggtcagcgtt gccgccgata ttatggcgct ggtgctgtta actgcgccgg gtaaacaggg
    19261 cgcggatatt gtttttggtt cggcgcaacg cttcggcgtg ccgatgggct acggtggccc
    19321 acacgcggca ttctttgcgg cgaaagatga atacaaacgc tcaatgccgg gccgtattat
    19381 cggtgtatcg aaagatgcag ctggcaatac cgcgctgcgc atggcgatgc agactcgcga
    19441 gcaacatatc cgccgtgaga aagcgaactc caacatttgt acttcccagg tactgctggc
    19501 aaacatcgcc agcctgtatg ccgtttatca cggcccggtt ggcctgaaac gtatcgctaa
    19561 ccgcattcac cgtttgaccg atatcctggc ggcaggcctg caacaaaaag gtctgaagct
    19621 gcgccatgcg cactatttcg acactctgtg tgtggaagtg gccgacaagg cgggcgtact
    19681 ggcgcgtgcc gaagcggctg aaatcaacct gcgtagcgat atcctgaacg cggttgggat
    19741 cacccttgat gaaaccacca cgcgtgaaaa cgtaatgcag cttttcagcg tgctgctggg
    19801 cgataaccac gggctggaga tcgacacgct ggacaaagac gtggctcacg acagccgctc
    19861 tattcagcct gcgatgctgc gcgacgacga aatcctcact catccggtgt ttaatcgcta
    19921 ccacagcgaa accgaaatga tgcgctatat gcactcgctg gagcgtaaag atctggcgct
    19981 gaatcaggcg atgatcccgc tgggttcctg caccatgaaa ctgaacgccg ccgccgagat
    20041 gatcccaatc acctggccgg aatttgccga actgcacccg ttttgcccgc cggaacaggc
    20101 tgaaggttat cagcagatga ttgcgcagct ggctgactgg ctggtgaaac tgaccggtta
    20161 cgacgccgtt tgtatgcagc cgaactccgg cgcacagggc gaatacgcgg gcctgctggc
    20221 gattcgtcat tatcatgaaa gccgcaacga agggcatcgc gatatctgcc tgatcccggc
    20281 ttctgcgcac ggaactaacc ccgcttctgc acatatggca ggaatgcagg tggtggttgt
    20341 ggcgtgtgat aaaaacggca acatcgatct gactgatctg cgcgcgaaag cggaacaggc
    20401 gggcgataac ctctcctgta tcatggtgac ttatccctct acccacggcg tgtatgaaga
    20461 aacgatccgt gaagtgtgtg aagtcgtgca tcagttcggc ggtcaggttt accttgatgg
    20521 cgcgaacatg aacgcccagg ttggcatcac ctcgccgggc tttattggcg cggacgtttc
    20581 gcacctcaac ctgcataaaa ctttctgcat tccgcacggc ggtggtggtc cgggtatggg
    20641 accgatcggt gtgaaagcgc atctggcacc gtttgtaccg ggtcatagcg tggtgcaaat
    20701 cgaaggcatg ttaacccgtc agggcgcagt ttctgcggca ccgttcggta gcgcctctat
    20761 cctgccaatc agctggatgt acatccgcat gatgggcgca gaagggctga aaaaagcaag
    20821 ccaggtggca atcctcaacg ccaactatat tgccagccgc ctgcaggatg ccttcccggt
    20881 gctgtatacc ggtcgcgacg gtcgcgtggc gcacgaatgt attctcgata ttcgcccgct
    20941 gaaagaagaa accagcatca gcgagctgga tattgccaag cgcctgatcg actacggttt
    21001 ccacgcgccg acgatgtcgt tcccggtggc gggtacgctg atggttgaac cgactgaatc
    21061 tgaaagcaaa gtggaactgg atcgctttat cgacgcgatg ctggctatcc gcgcagaaat
    21121 tgaccaggtg aaagccggtg tctggccgct ggaagataac ccgctggtga acgcgccgca
    21181 cattcagagc gaactggtcg ccgagtgggc gcatccgtac agccgtgaag ttgcggtatt
    21241 cccggcaggc gtggcagaca aatactggcc gacggtgaaa cgtctggatg atgtttacgg
    21301 cgaccgtaac ctgttctgct cctgcgtacc gattagcgaa tacctgtaat tcactgattc
    21361 gactattttc taaaggcgct tcggcgcctt tttagtcaga tgacaaagta caaaagtgct
    21421 cagacagtcc cctcgcccct ttggggagag ggttagggtg aggggaacag gccggcactg
    21481 gcgcgaatat ttaccctcac cccggccctc tccctgaaag ggcgagggga aaagcgtgcc
    21541 aacattgaag attgagccag tttgttagca atctcaaaga tacgtcaacg aattaatttt
    21601 tctcggaaaa acaaatggct atagcacttg tgaccggtgg cagtcgcggc atcgggcggg
    21661 caactgcatt actgttggcg aaagaagagt atacggtggc ggttaattat caacaaaacc
    21721 tccatgcggc gcaggaagtg gtgaacttaa tcacgcaagc cggtggcaag gcattcgtgc
    21781 tccaggcgga tatcagcgac gaaaatcagg tcatcgcaat gtttacagca atcgatcagc
    21841 acgatgaacc gctagcagcg ctggtcaata acgccgggat cttgtttacc cagtgcaccg
    21901 ttgaaaacct taccgcagag cgaatcaacc gagtactttc caccaacgtg acgggatatt
    21961 ttctctgctg ccgcgaggcg gtaaaacgca tggcgcttaa aaatggtggc agtggcggcg
    22021 ctatcgtcaa tgtctcttcg gtggcctcac ggttgggttc gccaggggaa taggttgatt
    22081 acgcggcatc gaaaggggcg attgatacgt taaccaccgg actatcgctg gaagtcgccg
    22141 cgcaggggat ccgcgttaac tgcgtgcggc cagggtttat ttataccgaa atgcacgcca
    22201 gcggcggcga gcttggacgc gtcgatcgcg ttaagtcgaa catccccatg cagcgtggtg
    22261 gacaggcaga agaggtcgcg caggccattg tctggctact aagtgataaa gcctcttacg
    22321 tcacgggaag ttttatcaat ttggcgggcg ggaaataaaa cagggaagtt gtctgaccgg
    22381 atgcaacaag tattgcatcc ggtacttcat cgacttaaag cttctcgccg ttgctggcaa
    22441 tcacctcttt gtaccagtta aagctcttct tacgtgaacg cgacatatcg ccagtaccgt
    22501 cgtcatgttt attcacatag ataaagccgt agcgtttgct gtactgcccg gtagtgaatg
    22561 acacgcagtc gatgcagccc cacggtgtgt agcccatcag atccacacca tcgtaagtca
    22621 ctgctttttt catctcttca atatgggcgc gcaggtagtc aatgcggtag tcgtcgttga
    22681 tgctgccatc ttcttccact ttgtcgtaag cgccaaaacc gttttcgaca ataaacagcg
    22741 gcctctgata acgctcatac agttcgcaaa gggcatagcg cagacctact ggatcaatct
    22801 gccagcccca gtcagacgct ttaacatacg ggttcggtac gctgccttca aaaccagaga
    22861 tcgcatcgcc ggtgccgcct tcggccttca ctgcgttgat catgtaatag ctgaaaccaa
    22921 gataatcgca ggtgccttca cgcagtacat ccagatcgcc atcttccatt ttgatgttaa
    22981 atccgcgacg ctcccactcg ttcaacacat aggacgggta atagccgcgt agctgcacat
    23041 cggtaaagac gtagcgttcg cgcatcgact cctgagcgaa catcacatcg tccgggttac
    23101 aggagtaagg atagagcggc accatcgcca gcatacagcc gactttcatc tccgggttaa
    23161 tgcgacgcgc ggctttcact gctagggcgc tggcgacaaa ctggtgatgc agcacctggt
    23221 acatcgtctc ttccgggttg tcatgctcgg tatacaccac gccggagcag cagtaaccaa
    23281 acagcggcgc acgccagtta cgctggttgt ttatttcgtt gaaggtcatc cagtatttga
    23341 ctttatgctt gtagcgttcg aataccactt ccgcgaaacg gacaaagaaa tcaacgactt
    23401 tacggttggt ccagctaccg tattgctgca ccagatgcag cggtatttca aagtgggaga
    23461 gggttatcac cggttcgatg ttgtatttca gcagttcatc gaacagagag tcgtaaaact
    23521 tcagcccttc ttcgtttggc tgagcttcat caccttttgg aaaaatacgc gtccaggcga
    23581 tggaagtccg aaaacatttg aagcccattt cggcaaatag cttgatgtct tccttgtagt
    23641 gaccataaaa atcaacggct tcatggtttg gatagtattt tcctggcaag acttctttgg
    23701 tgatttcgcg cggcacgccg tgtgcgccac cggtcagaac gtcacaaatg ctcggtcctt
    23761 tgccgccttt gttccagccg ccttcgacct gatgagcggc aacggcgcca ccccataaga
    23821 aatctttcgg taaggtgagt tttttcacta tcataggctc cagatgagtc aattcttaca
    23881 ggaaatagta ccatgcagcc aaacgacatc actttttctc aacgtttcca ggatgacatt
    23941 ctggctgggc gtaaaaccat caccatccgc gacgagtctg aatcgcactt caaaacgggt
    24001 gatgtgcttc gtgtcggacg ttttgaagat gacggttatt tttgcacgat tgaagtcacc
    24061 gcaacctcaa ccgtaacgct ggatacgctg acggaaaaac atgcagagca ggaaaatatg
    24121 accctgactg aactgataaa ggtcattgcc gacatctatc ccggtcagac acagttttat
    24181 gtgattgaat ttaaatgtct ttaaattgaa ctcacgcctg ttagttgaaa tttctcattt
    24241 aatttcatga tttttaggag attagtctca aatcagaatt tattttagct aacaggtgtt
    24301 cactggaact attctcagtt acgctggaga ggtatacatg tgcgtgtacg tttctccgga
    24361 gtaagttatg gttcagaagc ccctcattaa gcagggatat tcgctggcag aggaaattgc
    24421 caacagcgtc agtcacggca ttgggttggt gtttggtatc gttgggctgg tgttgctact
    24481 ggttcaggcg gtggatctca atgccagcgc cacagcgata accagctaca gcctctatgg
    24541 cggcagtatg atcctgctgt tcctcgcttc gacgctctat cacgccattc ctcatcaacg
    24601 ggcaaaaatg tggctgaaga aatttgacca ttgcgctatt tacctgttga ttgccggaac
    24661 ctacacgccg tttttgctgg tggggctgga ttctccgtta gcgcgcgggt tgatgattgt
    24721 tatctggagc ctggcattgc tggggattct gtttaaactg accatcgcgc accgattcaa
    24781 aattttatct ctggtgacct atctggcgat gggctggctg tcgctggtgg taatttatga
    24841 aatggcagtt aagctcgcgg cgggcagcgt taccttactg gcggtaggcg gcgtggttta
    24901 ttcgctcgga gtgattttct atgtctgcaa acgcattccc tacaatcatg ccatctggca
    24961 cggcttcgtg ctcggcggca gtatgtgcca ctttctggcg atctatttgt atattgggca
    25021 ggcgtaatgg gtatgccttt gtaggcctga taagacgcgc cagcgtcgca tcaggcatcg
    25081 gagcgcaacc gccggatgcg gcgtgaacgc cttatccgat ctacgggcct cgtccctgtt
    25141 gtgataaagg catcaaatcc cagacatgat aagacgcgtc agcgtcgcat caggcgataa
    25201 atgcctgatg cgatacagag atttactctt cgagtgaata cggcagcggc tcgatatgca
    25261 gtgtattcgc atcgtcgcgt acgcggaaga tgctgtccgg ttccatatcg ttattcatga
    25321 cgacctgtac cacgacctga ccatcttcca gttttaccgc agccagcacc gtaccggtac
    25381 gacgccagtt ctcgcccatt ttcagctcta agtcttcacc agcttccggc agtcggctgg
    25441 cgctacctgc cagcaaccag agcgcacgtt tattggcacc acggaatttt gctcgcgcca
    25501 ccatctcttg tccggtataa cagcctttct taaaactgat accgcccagc gcctggagat
    25561 tggtcgcctg tgggataaac tgcccgctgt tcgcggcatc aatcaccggg aaacccgctt
    25621 caatgtttaa tgccagccac tgttggctat tgttcagttc cgcttcaccg cgcagtttat
    25681 cggtcagcat attggcagta gcttcatcgg ttacgatcag gaaacgttct gccgggtgtt
    25741 caaaccatag caaagtggtc gcgccttctt taactacctg tttttctctc gaaggtagtt
    25801 cactaaagag attggcgagc gcggcgcgcg cctgaaaacc ggcaacacca agcagcacac
    25861 gctcgtcgtc tgccgcgatg gtcactttag agaacaccgc atattttttc agttcagtca
    25921 gctgcggttc acgcacgctg cgccgttcaa tccacgcaaa gccatcgccg tcgcggaaca
    25981 gacgtaaatt gctccacatt ttacctttgg cgtcgcaatg ggcggcgagc aggtgctgat
    26041 tttctgtcat ctggctgaca tctgctgtca cctgaccctg catatatttt tcgctgtccg
    26101 cgccagtaat ggtggcaagc gcccagtcat caagcgtcat cagcgtcagc ggtaaacggg
    26161 cagaagccgt aggctgacgg ggaggaaaag gtgtaaaagc cataatagag tcctgagttg
    26221 cttaacggcg tattaataac taatggtaaa agagctatga ggcaatgcaa gtgctttcac
    26281 tacgcgatct gtgccttctg cgaggcgact tccaaaaaaa tgaagctctt gaattgttgt
    26341 gaattaattt cgcgatcgcg atggtgttta ctgatattcc cgtaaaagaa gtcgaaaaac
    26401 acgttacaat agccgggtac tggattttcg tgagacagga agaagatgga cattaacaac
    26461 aaagcccgca ttcattgggc atgccgccgt ggtatgcgcg aactcgatat ttcaatcatg
    26521 ccgtttttcg aacatgagta cgacagctta agcgatgacg aaaaacgcat ctttattcgt
    26581 ctgctggaat gtgacgatcc ggacctgttt aactggctga tgaatcacgg taaaccggcc
    26641 gatgcagaac tggaaatgat ggtccgactc atccagacac ggaaccggga acgtggtcct
    26701 gtggcaatct gatttgcgcg tctcctggcg cgcacagtgg ctttccttgc tgattcatgg
    26761 gctggttgcc gctgttattt tactcatgcc ctggccgctc agttacaccc cgttatggat
    26821 ggtgttactt tcgctggtgg tatttgattg cgttcgcagc cagcggcgta ttaatgctcg
    26881 ccagggggaa attcgattgt tgatggacgg gcgtttgcgt tggcaagggc aggagtggag
    26941 catcgtcaaa gcaccgtgga tgattaagag cggcatgatg ctgcgtttac gttctgatgg
    27001 cggtaaacgg caacatttat ggctggcagc cgacagcatg gacgaagctg aatggcggga
    27061 tttacggcgg attttgttgc aacaagagac gcaaagataa atatgacggc aaagcatatg
    27121 cgctaagccg tcagtgagat caggcgtaat gctctgccat ttcgttgagg atttgctcgc
    27181 accagctctg aatacgctcg tcgctaaggt catactggtt agtttcatcc agcgccagac
    27241 ccacgaacag ttgcccgtca gcaatcaccg gtttcgggct ggtaaattca tatccttccg
    27301 ttggccagta gccgacgaac ttcacgcctt tggtcgagag tttgtcatgc agcataccga
    27361 gcgcatcgag gaaccactcg ccgtatccca gttgatcgcc aagcccatac agcgcaacaa
    27421 ttttaccttc aaggttcagg tcgtcgagct gatcccagac ggcttcccag tcttcctgga
    27481 tttcgccaaa atcccaggtc gggataccca gaatgagcac atcgtactgc tccattaatt
    27541 tcggggagtc gtccttgagg ttatgtaagg tcaccagttc tgggccgata atatcgcgga
    27601 ttttttctgc cgccatttcg gtgtaacagg tgctggaacc gtaaaaaaga cccatattca
    27661 taacgtaagc ttctcgattc tgctgtggct tttgcggcta gtgtatcaga atcgctttag
    27721 tcacggcaca ataaggcata atggatcaaa tgagtgacca taaggggcgc aagtgaaaca
    27781 ggatctggca cgcatcgagc agtttcttga tgctctgtgg ctggaaaaaa atctggctga
    27841 aaatacgttg aacgcttacc gtcgcgatct gtcaatgatg gtggagtggt tgcatcaccg
    27901 cgggttgacg ctggcgacgg cgcaaagtga cgatttgcag gcattactgg cagaacggct
    27961 agagggcggg tataaagcca ccagctcagc gcgtttgctg agtgcagtgc gccgattgtt
    28021 ccagtatctt tatcgcgaaa agtttcgtga agacgatccc agtgcgcatc tcgcttcacc
    28081 gaaattgcca cagcgtttgc caaaagattt aagcgaagcg caggtcgaac gtttattaca
    28141 ggcaccatta attgatcagc cactggagct acgcgataaa gccatgcttg aagtgttgta
    28201 tgctaccggg ctgcgtgtct ctgaactggt cgggctgaca atgagtgata tcagcctacg
    28261 tcagggcgta gtgcgggtca ttggtaaagg caacaaagag cgcctggtgc cgttaggtga
    28321 agaggccgtt tactggctgg aaacctatct ggaacatggg cgtccgtggc tgttgaatgg
    28381 tgtgtcaatt gacgtgttgt ttcccagcca gcgtgcgcag cagatgacgc gacagacctt
    28441 ctggcaccgc attaaacatt atgccgtgct ggcgggtatc gacagcgaaa agctgtcacc
    28501 gcatgtgttg cgtcacgctt ttgccactca tttattaaat catggtgcgg atttacgcgt
    28561 ggtgcagatg ctactgggcc acagcgatct ctccaccacg caaatttata cgcatgtcgc
    28621 taccgagcgt ctgcggcaac ttcatcaaca gcatcacccg cgggcgtgat gtctgaaaag
    28681 aacgggaaga tttatgaaga aaggttttat gttgtttact ttgttagcgg cgttttcagg
    28741 ctttgctcag gctgatgacg cggcaattca acaaacgtta gccaaaatgg gcattaaaag
    28801 cagcgatatt cagccagcac ccgtggctgg catgaagact gtgctgacta acagcggtgt
    28861 gttgtacatc accgatgacg gtaaacatat cattcagggg ccaatgtatg acgttagtgg
    28921 cgcggctccg gtcaatgtca ccaataaaat gctgctaaag cagttgaatg cgcttgaaaa
    28981 agagatgatc gtttataaag cgccgcagga aaaacacgtc atcaccgtgt ttactgatat
    29041 tacctgtggg tactgccaca aactgcatga gcaaatggca gactacaacg cgctggggat
    29101 caccgtgcgt tatcttgctt tcccgcgcca ggggctggag agccaggctg agcaacaaat
    29161 gaaagccatc tggtgtgcga aagataaaaa caaagcgttt gatgatgtga tggctggtaa
    29221 agctgccact ccggcaagtt gcgatatcga tattgccgat cattacgcac ttggcgtcca
    29281 gcttggcgtt agcggcactc cggcaattgt gctgagcaat ggcacacttg ttccgggtta
    29341 ccagccgccg aaagagatga aagaattcct cgacgaacac caaaaaatga ccagcggtaa
    29401 ttaattcgcg tgaaacaaca gatacaactt cgtcgccgtg aagtcgatga aacggcagac
    29461 ttgcccgctg aattgcctcc cttgctgcgc cgtttatacg ccagccgggg agtacgcagt
    29521 gcgcaagaac tggaacgcag tgttaaaggt atgttgccct ggcagcaact aagcggtgtc
    29581 gaaaaggccg ttgagatcct ttacaacgct tttcgcgaag gaacgcggat tattgtggtc
    29641 ggtgatttcg acgccgacgg cgcgaccagc acggctctaa gcgtgctggc gatgcgctcg
    29701 cttggttgca gcaatatcga ctacctggta ccaaaccgtt tcgaagacgg ttacggctta
    29761 agcccggaag tggtcgatca ggcccatgcc cgtggcgcgc agttaattgt cacggtggat
    29821 aacggtattt cctcccatgc gggggttgaa cacgctcgct cgttgggcat tccggttatt
    29881 gttaccgatc accatttgcc aggcgaaaca ttacccgcgg cggaagcgat cattaaccct
    29941 aacttgcgcg actgtaattt cccgtcgaaa tcactggcag gcgtgggtgt ggcgttttat
    30001 ctgatgctgg cgctgcgcac ctttttgcgc gatcagggct ggtttgatga gcgcggcatc
    30061 gcaattccta acctggcaga actgctggat ctggtcgcgc tggggacagt ggcggacgtc
    30121 gtgccgctgg acgctaataa tcgcattctg acctggcagg ggatgagtcg catccgtgcc
    30181 ggaaagtgcc gtccagggat taaagcgctg ctggaagtgg caaaccgtga tgcacaaaaa
    30241 ctcgccgcca gcgatttagg ttttgcgctg gggccacgtc tcaatgctgc cgggcgactg
    30301 gacgatatgt ccgtcggtgt ggcgctgttg ctgtgcgaca acatcggcga agcgcgcgtg
    30361 ctggcaaatg aactcgatgc gctaaatcag acgcgaaaag agatcgaaca aggaatgcag
    30421 attgaagcct tgaccctgtg cgagaaactg gagcgaagtc gcgacacgct acccggcggg
    30481 ctggcaatgt atcaccccga atggcatcag ggcgttgtcg gtattctggc ttcgcgcatc
    30541 aaagagcgtt ttcaccgtcc ggttatcgcc tttgcgccag caggtgatgg tacgctgaaa
    30601 ggttccggtc gctccattca ggggctgcat atgcgtgatg cgctggagcg attagacaca
    30661 ctctaccccg gtatgatgct gaagtttggc ggtcatgcga tggcggcggg tttgtcgctg
    30721 gaagaggata aattcgaact ctttcaacaa cggtttggcg agctggttac cgagtggctg
    30781 gacccttcgc tattgcaagg cgaagtggtg tcagacggcc cgttaagccc ggccgaaatg
    30841 accatggaag tggcgcagct gctgcgcgat gctggcccgt gggggcagat gttcccggag
    30901 ccgctgtttg atggtcattt ccgtctgctg caacagcggc tggtgggcga acgtcatttg
    30961 aaagtcatgg tcgaaccggt cggcggcggt ccgctgctgg atggtattgc ttttaatgtc
    31021 gataccgccc tctggccgga taacggcgtg cgcgaagtgc aactggctta caagctcgat
    31081 atcaacgagt ttcgcggcaa ccgcagcctg caaattatca tcgacaatat ctggccaatt
    31141 tagcgtcatc ttctctataa aaaagagcgt ggattgggta caatcccgct cttatcaccg
    31201 cattttgact agctcaataa aagaaatcag accatgtttg aaattaatcc ggtaaataat
    31261 cgcattcagg acctcacgga acgctccgac gttcttaggg ggtatctttg actacgacgc
    31321 caagaaagag cgtctggaag aagtaaacgc cgagctggaa cagccggatg tctggaacga
    31381 acccgaacgc gcacaggcgc tgggtaaaga gcgttcctcc ctcgaagccg ttgtcgacac
    31441 cctcgaccaa atgaaacagg ggctggaaga tgtttctggt ctgctggaac tggctgtaga
    31501 agctgacgac gaagaaacct ttaacgaagc cgttgctgaa ctcgacgccc tggaagaaaa
    31561 actggcgcag cttgagttcc gccgtatgtt ctctggcgaa tatgacagcg ccgactgcta
    31621 cctcgatatt caggcggggt ctggcggtac ggaagcacag gactgggcga gcatgcttga
    31681 gcgtatgtat ttgcgctggg cagaatcgcg tggtttcaaa actgaaatca tcgaagagtc
    31741 ggaaggtgaa gtggcgggta ttaaatccgt gacgatcaaa atctccggcg attacgctta
    31801 cggctggctg cgtacagaaa ctggcgttca ccgcctggtg cgtaagagcc cgtttgactc
    31861 cggcggtcgt cgccacacgt cgttcagctc cgcgtttgtt tatccggaag ttgatgatga
    31921 tattgatatc gaaatcaacc cggcggatct gcgcattgac gtttatcgcg cgtccggcgc
    31981 gggcggtcag cacgttaacc gtaccgaatc tgcggtgcgt attacccaca tcccgaccgg
    32041 gatcgtgacc cagtgccaga acgaccgttc ccagcacaag aataaagacc aggccatgaa
    32101 gcagatgaaa gcgaagcttt atgaactgga gatgcagaag aaaaatgctg agaaacaggc
    32161 gatggaagat aacaaatccg acatcggctg gggcagccag attcgttctt atgtccttga
    32221 tgactcccgc attaaagatc tgcgcaccgg ggttgaaacc cgcaatacgc aggccgtgct
    32281 ggacggcagc ctggatcaat ttatcgaagc aagtttgaaa gcagggttat gaggaaccaa
    32341 catgtctgaa caacacgcac agggcgctga cgcggtagtc gatcttaaca atgaactgaa
    32401 aacgcgtcgt gagaagctgg cgaacctgcg tgagcaggga attgccttcc cgaacgattt
    32461 ccgtcgcgat catacctctg accaattgca cgcagaattc gacggtaaag agaacgaaga
    32521 actggaagcg ctgaacatcg aagtcgccgt tgctggccgc atgatgaccc gccgtattat
    32581 gggtaaagcc tcttttgtta ccctgcagga cgttggcggt cgtattcagc tgtacgttgc
    32641 ccgtgacgat cttccggaag gcgtttacaa cgagcagttc aaaaaatggg atctcggcga
    32701 catcctcggc gcgaaaggta aactgttcaa aaccaaaacc ggcgaactgt ctatccactg
    32761 caccgagctg cgtctgctga ccaaagcact gcgtccgctg ccggacaaat tccacggctt
    32821 gcaggatcag gaagcgcgct atcgtcagcg ttatctggat ctcatctcta acgatgaatc
    32881 ccgcaacacc tttaaagtgc gttcgcagat cctctctggc attcgccagt tcatggtaaa
    32941 tcgcggcttt atggaagttg aaacgccgat gatgcaggtg atcccaggcg gtgccgctgc
    33001 gcgtccgttt atcacccatc ataacgcgct ggatcttgac atgtacctgc gtatcgcgcc
    33061 ggaactgtac ctcaagcgtc tggtggtcgg tggcttcgag cgtgtattcg aaatcaaccg
    33121 taacttccgt aacgaaggta tttccgtacg tcataaccca gagttcacca tgatggaact
    33181 ctatatggct tacgcagatt acaaagatct gatcgagctg accgaatcgc tgttccgtac
    33241 tctggcacag gatattctcg gtaagacgga agtcacctac ggcgacgtga cgctggactt
    33301 cggtaaaccg ttcgaaaaac tgaccatgcg tgaagcgatc aagaaatatc gcccggaaac
    33361 cgacatggcg gatctggaca acttcgactc tgcaaaagcg attgctgaat ctatcggcat
    33421 ccacgttgag aagagctggg gtctgggccg tatcgttacc gagatcttcg aagaagtggc
    33481 agaagcacat ctgattcagc cgaccttcat tactgaatat ccggcagaag tttctccgct
    33541 ggcgcgtcgt aacgacatta acccggaaat cacagaccgc tttgagttct tcatcggtgg
    33601 tcgtgaaatc ggtaacggct ttagcgagct gaatgacgcg gaagatcagg cgcagcgctt
    33661 cctggatcag gttgccgcga aagacgcagg tgacgacgaa gcgatgttct acgacgaaga
    33721 ttacgtcacc gcactggaac acggcttacc gccgacagca ggtctgggaa ttggtatcga
    33781 ccgtatggta atgctgttca ccaacagcca taccatccgc gacgttattc tgttcccggc
    33841 gatgcgtcca gtaaaataag cattacgtta tgctaataac cccggcaaat gtcggggttt
    33901 tttaattgtg ctgcgcgaaa gcagacaaca attttcttgc ttcactattg gctgcctgca
    33961 ttaccatcca cggactgaac gcccacggcg tggcatcaat accgtgtaat acatctgcta
    34021 aatcacacca ttgataatcc atcacttcat catcgttgat ctgtaacgca ctgttggtgc
    34081 gtgcggcaaa taccggacac acttcatttt ccacaatgcc attcggatcg gtggcgcgat
    34141 agcgaaagtc aggatagata gattcaggag gcgtaatttc cacgccaagc tcataacggc
    34201 aacggcggat cactgcgtct tcgttgcttt ctcccagttg tgggtgccca caaaccgagt
    34261 tagtccacac gccaggccat gcttttttgc tcagtgcgcg gcgggtaact aataattgcc
    34321 ccttggcatt aaacagccaa ctggagaacg cgagatgtaa gagggtgtct gccgtgtgtg
    34381 cggcatactt ttccagcgta cccgtgggaa ctccctgtgc attcaataaa atgacgtgtt
    34441 ccgtttgcat aatttctcac atgtaattct gatcactcat cattatagat aattgaagag
    34501 tgaaatgatt aaccaagtga gcgtttatcg acaaccgccc gttttgagcg gatgccgaca
    34561 ggtaaaaacc atttaaaaaa gtagcatcat atatttgaag cgactttcaa tggccattgc
    34621 aacacagtca gaaatgacag gaatagagag agtaaaaagc aacagcagac tgaatatagc
    34681 gcgcattaaa agatttatat tattctccat gctcattttt cttcagcttg cgcatcagaa
    34741 gccacatacg tactgtcctg acttttaaat ctttatcaga gacggattcc ttcaccaggt
    34801 cagcatgatg ctgatagcca ttaaagaaga cgttcagcac aacggcgcta agggttgcga
    34861 gcataatgcc gctatgtagc agcggttgca gtacggccgg taacttagaa aagaaatcgt
    34921 gagagagcgt cggagtcatg ccaacgccga gactgatcgc cacaatatag aggttgtaac
    34981 ggttggtggt gtagttacag cgcgacagaa ttcgaatccc tgtcgccagt accatgccga
    35041 acatcactag accagcgccg cccagcacaa attgcggaat ggaggctacc agcaccgcca
    35101 tttttggcac catgccaaac aggattaata taattcccga agaaatacac acccagcggc
    35161 tatgaacgcg cgtcacgcta accaggccaa cgttttgaga aaatgacgtg tgggggaagc
    35221 tgttaaacgt gccgcctatc attgtcccta cgccatcgac acgcagcccg cgaataatat
    35281 cgtgtgaaga gagtttacga ccgactattt cacccagtgc caggaacatc cccatcgact
    35341 cgataaacac gatgattaac acggcagtca tggtcagaat ggaaacggga tcgaaaatcg
    35401 gcataccaaa cgacatcggg gtgacaatcg caaaccacaa ggcatcatgt agcccggata
    35461 aattgacttc attcatcatc cacgaaagta aaaagccaaa tacaatcccc agtaatacgg
    35521 cgacgttgga cataaatcct ttcgcatagc gagtaatgag caagataaaa attaagacgg
    35581 caaaggagat acctgaataa acgggattac catattgcgg attcccttta cctccggcgg
    35641 cccagtcaat acccacctga atgatgctaa gtccgacaga agtaataacc acaccggtaa
    35701 ccagtggcgg gaataaaggc atcaagcgac cgataagtgg cgctaataat gtggtgataa
    35761 aacccgcggc gatagtggca ccaaatatcc ccagcaggcc aatatccggg ttcatcccaa
    35821 tggctatcat tggtgttaca gcagcaaagg tcaccgacat aatcaccggc aggcggatcc
    35881 ccataaagcg gccgataccg atacattgca ataatgtgac gatcccgcag caaaagagat
    35941 ccgagctaat gagcatcgca atagcttctt ttgagaggcc cagtcggtca ccaatcataa
    36001 gaggaacagc gactgcacct gcgtacatga caaggacgtg ttgcagaccg agtatgatca
    36061 gctttcctgg tgataatatg cggtcaacct catcagttgg gcggtcttgc ccagaagatg
    36121 agagaagttg ggaatctatg gcgctcatca gtgtttctcc tttcgcccgc cagagtaaaa
    36181 agcaaccgtt tacgttaatg aaactgtcta aacacatgct cgtggagtgc cagtttttgc
    36241 gaggaagggg agttagagac aggaattgcg gaggcaatca cataatcgtg gttaaaccac
    36301 gaaaatgaga agagaacctg acaatggaat tatctgtaat tttctcatat tgatatttca
    36361 ttaatcattc tggcatgtcg tactctcgca tttaatcgtt tttatctgga tagcactctt
    36421 ttgatcggca atgttgaaat aatgaaagcg gcatagattg aggggcaata atttgaactc
    36481 agtcgcaaaa ttataattaa aacggtcatg ctacctaata tatgcccgtt tat
//
LOCUS       DAJTMC010000021        35888 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.021, whole genome shotgun sequence.
ACCESSION   DAJTMC010000021 DAJTMC010000000
VERSION     DAJTMC010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35888
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.021"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            108..455
                     /gene="grxD"
                     /locus_tag="OO130_000926"
     CDS             108..455
                     /gene="grxD"
                     /locus_tag="OO130_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HCR5364429.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            complement(506..4913)
                     /locus_tag="OO130_000927"
                     /pseudo
     CDS             complement(506..4913)
                     /locus_tag="OO130_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
     gene            complement(5006..5653)
                     /gene="rnt"
                     /locus_tag="OO130_000928"
     CDS             complement(5006..5653)
                     /gene="rnt"
                     /locus_tag="OO130_000928"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HCR5364430.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRSAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMIAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            complement(5756..6163)
                     /gene="gloA"
                     /locus_tag="OO130_000929"
     CDS             complement(5756..6163)
                     /gene="gloA"
                     /locus_tag="OO130_000929"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HCR5364431.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFMEDPDGYKIELIEEKDAGRGLGN"
     gene            complement(6244..7341)
                     /gene="nemA"
                     /locus_tag="OO130_000930"
                     /pseudo
     CDS             complement(6244..7341)
                     /gene="nemA"
                     /locus_tag="OO130_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
     gene            complement(7378..7977)
                     /gene="nemR"
                     /locus_tag="OO130_000931"
     CDS             complement(7378..7977)
                     /gene="nemR"
                     /locus_tag="OO130_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416166.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HCR5364432.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYTAYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            8080..8319
                     /locus_tag="OO130_000932"
     CDS             8080..8319
                     /locus_tag="OO130_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HCR5364433.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            8368..9264
                     /locus_tag="OO130_000933"
     CDS             8368..9264
                     /locus_tag="OO130_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HCR5364434.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RDHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            9345..9866
                     /gene="sodC"
                     /locus_tag="OO130_000934"
     CDS             9345..9866
                     /gene="sodC"
                     /locus_tag="OO130_000934"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC2"
                     /protein_id="HCR5364435.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGAGHLGDLPALVVNNDGKATDAVITPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(9867..11878)
                     /locus_tag="OO130_000935"
                     /pseudo
     CDS             complement(9867..11878)
                     /locus_tag="OO130_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000994342.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
     gene            complement(11878..12735)
                     /locus_tag="OO130_000936"
     CDS             complement(11878..12735)
                     /locus_tag="OO130_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR5364436.1"
                     /translation="MSIKTIKYFSIIIVAIVAVLAGWWLWNYYMQSPWTRDGKIRAEK
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LRGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            complement(12801..12974)
                     /locus_tag="OO130_000937"
     CDS             complement(12801..12974)
                     /locus_tag="OO130_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000670993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HCR5364437.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPL"
     gene            13175..13609
                     /gene="slyA"
                     /locus_tag="OO130_000938"
     CDS             13175..13609
                     /gene="slyA"
                     /locus_tag="OO130_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HCR5364438.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            complement(13656..14123)
                     /gene="slyB"
                     /locus_tag="OO130_000939"
     CDS             complement(13656..14123)
                     /gene="slyB"
                     /locus_tag="OO130_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HCR5364439.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            14397..15506
                     /gene="anmK"
                     /locus_tag="OO130_000940"
     CDS             14397..15506
                     /gene="anmK"
                     /locus_tag="OO130_000940"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HCR5364440.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VIRPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            15604..15933
                     /gene="mliC"
                     /locus_tag="OO130_000941"
     CDS             15604..15933
                     /gene="mliC"
                     /locus_tag="OO130_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HCR5364441.1"
                     /translation="MSMKKLLLICLPVLLTGCSAFNQLVEHMQTATLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGARYTDGIYVFWSKGDEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            15992..16648
                     /gene="pdxH"
                     /locus_tag="OO130_000942"
     CDS             15992..16648
                     /gene="pdxH"
                     /locus_tag="OO130_000942"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HCR5364442.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLLRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHAQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            16777..18051
                     /gene="tyrS"
                     /locus_tag="OO130_000943"
     CDS             16777..18051
                     /gene="tyrS"
                     /locus_tag="OO130_000943"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HCR5364443.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            18110..18973
                     /gene="pdxY"
                     /locus_tag="OO130_000944"
     CDS             18110..18973
                     /gene="pdxY"
                     /locus_tag="OO130_000944"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HCR5364444.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(19017..19622)
                     /gene="gstA"
                     /locus_tag="OO130_000945"
     CDS             complement(19017..19622)
                     /gene="gstA"
                     /locus_tag="OO130_000945"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HCR5364445.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     AVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLEKKLQYVNETLKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(19728..21230)
                     /gene="dtpA"
                     /locus_tag="OO130_000946"
     CDS             complement(19728..21230)
                     /gene="dtpA"
                     /locus_tag="OO130_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HCR5364446.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAIIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
     gene            complement(21841..22476)
                     /gene="nth"
                     /locus_tag="OO130_000947"
     CDS             complement(21841..22476)
                     /gene="nth"
                     /locus_tag="OO130_000947"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HCR5364447.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(22476..23171)
                     /gene="rsxE"
                     /locus_tag="OO130_000948"
     CDS             complement(22476..23171)
                     /gene="rsxE"
                     /locus_tag="OO130_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HCR5364448.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRTEAA
                     AERALPNGETGNV"
     gene            complement(23175..23795)
                     /gene="rsxG"
                     /locus_tag="OO130_000949"
     CDS             complement(23175..23795)
                     /gene="rsxG"
                     /locus_tag="OO130_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HCR5364449.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(23799..24857)
                     /gene="rsxD"
                     /locus_tag="OO130_000950"
     CDS             complement(23799..24857)
                     /gene="rsxD"
                     /locus_tag="OO130_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HCR5364450.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSTLLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGADMNTLHLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGLWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(24858..27080)
                     /gene="rsxC"
                     /locus_tag="OO130_000951"
     CDS             complement(24858..27080)
                     /gene="rsxC"
                     /locus_tag="OO130_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HCR5364451.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRTRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDARFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVADPRKTAVEAAIARA
                     KARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEEQVDPRKAAIE
                     AAIARAKARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAVPEEQVDP
                     RKAAVEAAIARAKARKLEQQQTNAEPEEQVDPRKAAVAAAIARAQAKKAAQQKVVNED
                     "
     gene            complement(27073..27651)
                     /gene="rsxB"
                     /locus_tag="OO130_000952"
     CDS             complement(27073..27651)
                     /gene="rsxB"
                     /locus_tag="OO130_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HCR5364452.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            complement(27651..28232)
                     /gene="rsxA"
                     /locus_tag="OO130_000953"
     CDS             complement(27651..28232)
                     /gene="rsxA"
                     /locus_tag="OO130_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HCR5364453.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFTAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            complement(28309..28749)
                     /locus_tag="OO130_000954"
     CDS             complement(28309..28749)
                     /locus_tag="OO130_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HCR5364454.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVSYFKRSSRVKATFVNP"
     gene            complement(28835..29050)
                     /gene="ydgT"
                     /locus_tag="OO130_000955"
     CDS             complement(28835..29050)
                     /gene="ydgT"
                     /locus_tag="OO130_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HCR5364455.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            complement(29323..29448)
                     /gene="blr"
                     /locus_tag="OO130_000956"
     CDS             complement(29323..29448)
                     /gene="blr"
                     /locus_tag="OO130_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HCR5364456.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            29661..30701
                     /locus_tag="OO130_000957"
     CDS             29661..30701
                     /locus_tag="OO130_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR5364457.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            complement(30736..31737)
                     /gene="add"
                     /locus_tag="OO130_000958"
     CDS             complement(30736..31737)
                     /gene="add"
                     /locus_tag="OO130_000958"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HCR5364458.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLHYVELRFSPG
                     YMAMAHKLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNLARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRAGIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(31840..33012)
                     /gene="malY"
                     /locus_tag="OO130_000959"
     CDS             complement(31840..33012)
                     /gene="malY"
                     /locus_tag="OO130_000959"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HCR5364459.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDSQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHGVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRIYLKDNLTYIADKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            complement(33022..34614)
                     /gene="malX"
                     /locus_tag="OO130_000960"
     CDS             complement(33022..34614)
                     /gene="malX"
                     /locus_tag="OO130_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HCR5364460.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQTQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPAAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYAIFRFAITRFNLKTPGRDSEVASSIEKAVAAAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            34789..35817
                     /gene="malI"
                     /locus_tag="OO130_000961"
     CDS             34789..35817
                     /gene="malI"
                     /locus_tag="OO130_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HCR5364461.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGTTLADRMMQKITHEETHSRNLII
                     PARLIAAK"
BASE COUNT         8993 a         9226 c         9339 g         8330 t
ORIGIN      
        1 aaagggataa aatagcaaaa gcgtgatgaa aatgttattt tccgatgccg cactgacccg
       61 ctacaatgtc agattactgc cgtaaagaag tttgaggaag caagacaatg agcaccacta
      121 tcgaaaaaat ccaacgccag attgctgaaa acccgatcct gctgtacatg aaaggttcac
      181 cgaaactgcc gagctgcggt ttctctgccc aggcagtcca ggcgcttgcc gcatgtggcg
      241 aacgttttgc ctatgttgat attctgcaga atccggacat tcgtgcggaa ctgccgaaat
      301 atgctaactg gccgaccttc ccgcaactgt gggttgacgg cgagctggtc ggcggttgtg
      361 atatcgtgat cgaaatgtat cagcgtggcg aactgcagca gctgatcaaa gaaactgccg
      421 ctaaatacaa gtctgaagag ccggacgcgg aataatccgt cctgcaatac tgacggatgc
      481 gaacgggcat ccgtcagtcc tttctctatc cccaatccag cccttgtggc gaacttgaaa
      541 atcccgcctc acgcagcagc gtaaacatcg gcgtttgccg gaccggtaga tcattcactt
      601 ctgttagcgt aaagcgcagc cgtggttcgc gacgcagtgc ggtagtcagc gcgtggaaaa
      661 cctctggggc ttttcctgcc agaccagcat ttttttgcca ccttgcgcca gatagagtaa
      721 caatttgccg ctagaaacga ccaccagcgc acccgcccga cgcgttggaa ccagcgttgc
      781 cggatgtgca ggccagggca gaagatttcc ccacacatta gccggatcgt tggcagagag
      841 cgccactggt gtatagtggc gcgtttgcgt ggcttgtgtc gccagatcgc gccatcggtc
      901 aatagtcaga cgttcagcga attgcgcgcc acccagacct tctacaaaac gacctcgcat
      961 aattcgcccg gagtcttcca tacttcgaca aagcgtttgc atcgatggaa acccgccagg
     1021 gatattttcg gctatcaccg cctgacgact gatgatgccg tagcggtcga gcatattttc
     1081 cgccagcgcc agcatccttt cggtatcgtt tagtggctcc acctgcaata acgaccagcg
     1141 tccagccaga tttggtgtgt tgtaagatac cagcggcgag acgggttgcg catagacagg
     1201 acgtccacga cgagcccggt gactgcggcg agttgaggtg cgtgcgttgg aactgctgcg
     1261 ggtgagggcg cgtaacggtg cccaaatgtc gctggtgatg acgccttgcc agaccagcgc
     1321 ccataacgcc tcttgcaggg cagagagatc aaccgattcg ccgattttgt cgcgtatccg
     1381 ttggctgatt tgttgtgcaa accaggctcc tccgtcagcc agaacagcga ctatcgcttg
     1441 ttgcagcgcc gaacgattcg cctgatccgc ttcggcagga gtgaacgatt ctgcagcata
     1501 ttcctgtaga tgcagtgcca ccaggccgtc atcttcaccc agtttttttt gccccgacca
     1561 gataaccgca ccggttgcca gtaattcatc gagcatttct gacgaatagt cgcgtacgcg
     1621 ggcaggcagg atctggcttt cccagagtga ggcgggtaaa ccgactccgg caagctgttc
     1681 gatcacccgc atcacgccat ctacgccctc ataaacgcct ggcgatgttg aggcaaagag
     1741 cgccgggcta ccatcggtgg cgggtaatac gccctgacgt tccagcagca atcgcgcata
     1801 ggtcgtggct gcaacgggac gcgtcgcttc tctggcggct tgcagcgagc gcaaacgcag
     1861 acgacgaaat acttcatcgc tcacccagat gtcttgttgc agattcatca ccagaccctg
     1921 ttcacgcagt tgctgaagct gctcttcgac aatggcaata ccgagactaa actcatgagc
     1981 cagttgttca gccgtgacca gagcatgagc ccggagatag cgcagaaaga ggtcgcgaag
     2041 cgggtaactg actctatgta aataaatctc tggcaatgac tctggtagtc gtacgccgag
     2101 ggcatcacgc agcctggcgg catcatccat acaggcaaga cgctcctgtc cgctaatcat
     2161 cgctgggaag attcgtttta ctgcaagaag attttccaga tacgacgcaa cctcttcact
     2221 gctgcctgta tgccgttgcg ccaggtcttc aacggtcatt ggccccagtt cgcgcagcag
     2281 gtcgaacaat ccttcttcac ctttcgctct tctgccagga gccagtcgtt gcaactcttc
     2341 ttccacctgg cgaatgacct gcgggtcgag taattccccc ggatcgacct gtccgagtag
     2401 attgcgcagt aactcgctgt ccagcgacag tacggatgcc cggcgctctg ccagcggggc
     2461 gtcgctctgg tacataaatt ccgcgacata gccgaacaat aaacttgtgg cgaaaggcga
     2521 gggcgtagtg gtcgttacat cggatatttg aatttcgcca ccgttcaggc gacgcatcaa
     2581 acgttccagt gcgggaagat cataaacatc ttgcagacat tcgcgtagga tttcgagaat
     2641 gaccggaaaa tctggatatc cctgagcgat ttccagcaac tgactggcgc gcagccgttg
     2701 ttgccaaagc ggggtgcgat ggcccggagt gcgccccggc attaataatg cccgcgcggc
     2761 gcattcgcga aaacgggcgg cgaaaagtgc cgagctgcct accgcctcgc ggacaatttg
     2821 cagcaacttt tctggttcaa acaaaaaaat cgcggcgtcg ggcaatttac catcagtgtc
     2881 aggaatgcgc gcaacaatgc cgtcatcact ggcgaccaca gacgcgtcag cgcccagggt
     2941 tcatgcaccc gtcttccata gggagagtgc aaaataatac gccagtcgcc aatttcatca
     3001 cggcaccgtt cgaggaccag atggcgactc cccggaacga tgcccgtcgc gttgcgttgc
     3061 tcgtcaatca acccctgaat attggcgttc gtattttctt ctgccagcca cgggggaata
     3121 gtcccggaaa agaacgcgcc atccgccagt aaatgaagaa aatcgccgat catctcgcct
     3181 aattcagccg gacgtccgtt accttcacca cgccagaagg ggagccgggc agaacgaccc
     3241 ggagcaggag tcacaatcac ctgatcgcgg gtgatttgct ggatccgcca tgaggtagcg
     3301 ccgagagtga taatgtcgtt cacccgcgac tcatatacca tctcctcatc cagtttaccc
     3361 acccgccgcg aaccggcctt ttcttcacct tcgggtaata acacgctata catgccacga
     3421 tccggaatgg tgccgccgct ggtaaccgcc aacaattgag cgccaggtcg gacggtcaat
     3481 atcccggtct ccctgttcca gaccagtttg gggcgaaaag cagaaaaatc gccagaggga
     3541 tagcgcccgg aaagcatatc cagcgtggcg tcaaaaacac gtcttggcag atctttccac
     3601 ggtgcggcac ggcgtacgcg ggagtaccat tcgtctacct gtaatgcctc catcgccgcg
     3661 gcggcaacgg tttgctgcgc aaggacgtcg agtggattat gcggcggtgt caggttttcc
     3721 agcctgcctg cgaacataca ctctacaatg actgcggaat cgactaagtc acgccgggta
     3781 cgagggaaaa acagcccttt agatacaccg ccaacctgat gtccggcgcg accaatgcgt
     3841 tgtaacccac tggcaacaga aagcggcgtt gccacctgaa tcaccagatc cactgcgccc
     3901 atatcaatcc ccagttcgag actggaggtt gcgaccacgc agcgtaactc acccgatttc
     3961 agcgcctgct cggtgattgc tcgttgttct ttagagacgg agccgtggtg tgagcgggca
     4021 ataaaaacgt cgctactttg tacacggtta gaggttgcgc cggaggtcga ctcgaaatgg
     4081 gccgcatcaa cggcgataga cggggaacgc tgtaagcgtg cggcgtaaag ctcatttaat
     4141 cgtgccgtca gtttttccgc cagcccacgt gaattagtaa agacaatggt cgagcgatgg
     4201 cgcaacactt catcaaggat acccgtttca atatatggcc agatggagcc ttcccggccg
     4261 gcatggctgt cttcgccggt gctgctggcg accgatgaga catcatccat attggcgacc
     4321 ggtacgacaa ttcgtatctg cggatggcgc attgcgggcg ggttgactac cgtaaccggg
     4381 cgatcgccac caagaaaggc tgccacatcg ctggctgagc gcacagtggc agaaaggcca
     4441 attcgctgtg ctgaggtgtg gagcagcgca tcgagccgct ccagacttaa cgccagatgc
     4501 gcaccacgtt tactgcccgc taccgcgtgg acttcatcaa taattaccgt ttcgccgcgc
     4561 cgccgccgtt catcggcaat acccttcaac gggagctgca agttgcgctg aacgtcggtg
     4621 cccagagctt ttatcggtga aatatagagg atgcgtgagg ttttacgctt atgcgcctcg
     4681 cgggtatctt cgccgccttc gcggaagagc cgatcgaggg cgtagagaaa tgctgccagc
     4741 gttttcccgg agccggtcgg tgcaatcacc agcgcatgtt cgcttcgcgc cgccacatgc
     4801 caggtttgcg actggacagc ggtcggctgt ttaaaggcgc gaagaaacca gtcgcgggtc
     4861 gccggtgaaa acacgtccgg caggagcgat gaagggtctg gattatctgc cattaacgct
     4921 attccccgtg aatatcatgc aggatgcgtg gaatagcctc actatagatg cctgaatcat
     4981 tgcatgtcat caggcatcga ctcgattaca cctcttcggc ggcaggtagc ggccagcctc
     5041 ccagacgttt ccagcggttg acgatttcac aaaacagcac agcagtgcgt tcggtgtcgt
     5101 acagcgcgga gtgcgcctgg gtgctgtcga agtccatgcc agcggtctgg caagcctttg
     5161 acaatacggt ttgtccgagt gccagcccgg ccagtgcagc agtgtcaaaa gtggcgaaag
     5221 ggtggaacgg gttacgtttc agtgaggcgc gttctgcggc ggctatcata aagctgtgat
     5281 caaaattggc attgtgcgcc accataatgg cgcggttaca gccgctcgct ttaatacctt
     5341 tacgtacaac tttaaaaatt tcgtgcagcg cctcgtattc gctgaccgcg ctacgatcgg
     5401 gatcgttcgg gtcaatgccg ttgaaggcga gggcttctgg ttgcaaattt gcgccgacaa
     5461 atggttcgac gtggaaatgt aatgtggtgt ccggcatcag ccagccttgt tcatccattt
     5521 tcagggtgat ggcggcaatc tcaagcagcg catcggtttt ggcgttaaat ccggctgttt
     5581 caacatcgat cacaacagga taaaaaccac gaaaacggtc gcacagaccg gtaagttgag
     5641 cgttatcgga catctgattc tcttaatacg gataaattgc agcgcgcatt atgacaaata
     5701 ttacttgtcg atgcagtaaa gatgcgggcg cgatgagttc acgcccggca ggagattagt
     5761 tgcccagacc gcgaccggcg tctttctctt cgattaactc aattttgtaa ccgtccggat
     5821 cttccataaa cgcgataacc gtagtaccgc cttttaccgg acccgcttca cgggtcacgt
     5881 tacccccgtt ttgacggatt ttttcgcacg cttcagcggc gttatctacg ctaagcgcga
     5941 tgtgaccata agcagtgccg agttcgtatt tatccacgcc ccagttgtag gtcagttcaa
     6001 tcaccgcttc ttcggtttcc gggccgtagc caacaaacgc cagtgagtat ttgtattccg
     6061 ggttttcgct ggtacgcagc agtttcatgc ccagcacttt ggtataaaaa tcgatggagc
     6121 gttgcaaatc gccaacgcgc agcatggtat gaagaagacg catcttttta tcctcaaaat
     6181 ggctaacaga ttcaaaatgt tgttttagta tagcggcggc tttacgccgc tcgcaatgtt
     6241 ggattacaac gtcgggtaat cggtatagcc ttccgcgccg ccaccgtaga aactttcggc
     6301 acgctgtggg ttaagctcag ctttgcgctg caagcgggcg accagatccg ggttcgcaat
     6361 ccagtcacga ccaaatgcca ccgcatcaat taaccctttg ccgatcagcg tttcagcttt
     6421 ttctactgtg tatgcacctg cgccgataat cggaccgtgg aaacgggcgc gtactttttc
     6481 gcggaacgca tcagtatacg gttcaccccc cgcccaatct ggttctgaca tatgcagata
     6541 agcaatgccg cgtttaccca gttgttcaat cagatacagt gcatcggctt cttcattcgg
     6601 gccgttatcc gtgttctgga aagtaccgat tggcgaaatg cgaatgccaa tgcgatcggc
     6661 accccattct tcaatcccgg catcgaccac ttccagtacc agacgtgcgc gattttccac
     6721 gctgccgccg tactgatcgg tacgatggtt tgaagaagga gaaaggaact gatgcagcaa
     6781 ataaccgtga gcagagagga gctctaccag atcaaaaccg gcttcacgcg cgttagcaat
     6841 ggcctgacgg aaatcattga cgatacctgg aatctcttct agttcaagcg cacgcggcat
     6901 ggatgtttca acacggatcg cctgaccatt ttcatcgcgc agagaagtac gtgttcccgc
     6961 gctaagtgct gaaggcgcta ccggtgcctg accgccaggt tgcaggctgg cgtgagaaat
     7021 gcgtccggtg tgccacagct acacggccat atgaccattt tcagcatgaa cgccagcggt
     7081 gatttttttc catgcggcaa tttgctccgg actatggatg ccaggcgcac ctgcatatcc
     7141 ttttacctgg gcagaaattt gcgtggcttc actaataatc aaaccggcac tggcacgttg
     7201 gcgatagtat tccgccatca acggggtagg aatgtcaccc ggttcaatac tgcgcagacg
     7261 cgtcagcggt gccataaaaa tacggtttgc cgccgtgatc gcgcccactt tcagtgggga
     7321 atacagtttt tcagatgaca taaaggctcc cggtcgtctg gtgataaaaa atgcctgcta
     7381 aacggcaggc gtcgcaataa tgttttttac atgggccagc gcgttttcca gtggctcgaa
     7441 actgcgcgaa attttggcct gcagattcgc acccagccat agtgcgtaaa gcacttgtgc
     7501 ctgttgtagc ggttcgccac aaaaggttaa acaatggttc tcacggccat tttccagcgc
     7561 ctgagagagc agggcgatca cgccgcgagc acctttatcc atcgcgctgc gcatatcttc
     7621 tgacagatcg cacacttcgg cagagagttt tactgtcagg caaccactga tggttccatg
     7681 ttggcaaaac tggttcagtg tttgctggta ataagccagt atgcggtcgc ggtagttacc
     7741 ttcgccggat tgcagcaact cagtcagtcg ctggtgatat gcggtgtaat gacgctcaag
     7801 catggcaacg ccaaacgctt ctttagagcg aaagtagtga tagaaggacc ctttcggcac
     7861 ttcagcggtt tttagtaatt cgcttagccc catcccggtg aatccacgtt gcaggcaaag
     7921 ttgctcgccc gtcgccagga gatgttcgcg agtatcatgt tcggtgtgtt tgttcatgcg
     7981 ttggagtgta gtagaccagt cggtctagtg caagcaaagt tgctactttg gacattaaca
     8041 gcttcaataa tggtaataat cttatctgag gagggcgaag tggcggagca attagagttc
     8101 tttcccgtcc agagcccgtg ccggggaatt tgccagtctg acgaacgcgg gttttgtcgc
     8161 ggctgttttc gtagtcggga tgagcgtttt aactggaata aaatgagcga tggcgaaaaa
     8221 caagaggtgc tgcgcctttg tcggcagcga ttaatgcgca aattacgcgc aaataagcca
     8281 gcgtcatcag acgaaccgga gcaaccatca ctcttttgaa cctaatcttg cgtatactcc
     8341 tctcatatct gataagagga agcgattatg gttcagcgta ttactattgc gccgcaaggc
     8401 ccggagtttt cccgttttgt gatgggctac tggcgattga tggactggaa tatgtccgcc
     8461 cgccagctgg tcagttttat tgaagagcat ctggatctcg gcgtgaccac cgtggaccat
     8521 gctgatattt atggtggcta tcagtgcgaa gcggcgtttg gcgaggcact gaaactggca
     8581 cctcacctgc gtgaacggat ggaaatcgtc agtaaatgcg gtatcgcgac gaccgcgcgt
     8641 gaagaaaacg tcattggtca ttacatcact gaccgcgatc acatcattaa gagcgccgaa
     8701 cagtcgctaa ttaatctcgc gaccgatcat ctggatctgc tgttaatcca ccgaccagac
     8761 ccgttaatgg atgccgatga agtggcggac gcgttcaaac atctgcatca gagcggcaaa
     8821 gtgcgtcatt ttggcgtatc gaactttacg cctgcgcaat ttgccctgtt gcaatcacgt
     8881 ctgccgttta cccttgccac taatcaggtg gaaatatccc cggtgcatca gccgttactg
     8941 ctggatggca cgctcgacca actacaacaa ctgcgtgttc gtccgatggc gtggtcctgc
     9001 cttggtggtg gtcgtctgtt taatgatgat tatttccagc cgctgcgtga tgaactggct
     9061 gtggtggcag aggagttaaa cgcgggctcg attgaacagg tggtttacgc ctgggtatta
     9121 cgtttaccat cgcagccgct gccaattatc ggttcaggta aaattgagcg cgtacgggca
     9181 gctgtcgaag cagaaacact gaaaatgacc cgtcaacaat ggtttcgtat tcgtaaagcg
     9241 gcactggggt acgacgtacc gtaagcactt ttaggaatag ccgccgttca aaaatgtgtc
     9301 actggtttac acttattcag gaatgcacaa tgaacggagg tcctatgaaa cgttttagtc
     9361 tggctattct ggcgctggtt gttgcaaccg gcgcacaagc tgccagtgaa aaagtcgaga
     9421 tgaacctcgt cacgtcgcaa ggggtagggc agtcaattgg tagcgtcacc attactgaaa
     9481 ccgataaagg tctggagttt tcgcccgatc tgaaagcatt accccccggt gaacatggct
     9541 tccatattca tgccaaagga agctgccagc cagccaccaa agatggcaaa gccagcgccg
     9601 cggaatccgc aggcgggcat cttgatccac aaaataccgg taaacatgaa gggccagaag
     9661 gtgccgggca tttaggcgat ctgcctgcac tggtcgtcaa taatgacggc aaagctaccg
     9721 atgccgtcat cacgcctcgt ctgaaatcac tggatgaaat caaagacaaa gcgctgatgg
     9781 tccacgttgg cggcgataat atgtccgatc aacctaaacc gctgggcggt ggcggtgaac
     9841 gctatgcctg tggtgtaatt aagtaattaa gaggcctgcg taccttgcgg tggtgcttgc
     9901 tcaagttgcg aaagcgagca gtacagccgc cagacaattg ccgccagctc acgggcggca
     9961 ggttgatgat gacgggcaag gctgtcgcaa atccgctgta attcttcaag tgtggccgcc
    10021 agtgattttt gctgaacgcc acgctcactc atcactcccc gcaacagtga aatacagtta
    10081 tcccgtactc gcgataacgg atcggaacgc gattcccagt cgcgcaattg ccagacaaca
    10141 tgagaacagt tcagcagcac tacaccccag cgtaataacc aacggcgagc cagcgcatcc
    10201 tggctgttac tcaactgact gacgtgatga taagtgagcg attcaaattc gctttcactc
    10261 agtgttggat ggcggcttag ctgatcgaca aaatcccggc gcagcgcgcg aatatggcgg
    10321 cggcttttac gagcatccga tcctggacgc agaatggcga acgctagcca agccagcgca
    10381 acgccaacga tttttgccag attatcgtta agaaagtcag caaaatcata caccggggga
    10441 ttagtgacag cgataaacga acccataaaa acaatcagtt gcccccacag tgcggcaaat
    10501 ttgggcatct gcaatttaag aagctgcatt gtcgccagca gtggaaagag aaacagtaaa
    10561 aattgccaca gatcgctaat ctggaccatc aggccaaatt tgaccacaaa gctgaatagc
    10621 gaaagtaaca ccagcgtgcg catcagcagt gacaacgact taaacggtgc tgcgacggcg
    10681 gagtagagta cgcagctaat tgctgccagc gttaatgcat tggcaccggc atcctattgc
    10741 gatgcaatac tccatgcgcc aatcatcatc aacgtacaaa atgtacgcag cccgctccac
    10801 atagcttcgg cgttgtcggt atgacgagcc agagcggatg tccgtgcgag tctggcatga
    10861 tcatctacac cgctttgcag acgatgtaat tcctgactac tttgcagata aaggcggcaa
    10921 aaataacgta gtcgctgcca gaaggcgacg tgccggtagt cggcgacgtt ggtcgggcgt
    10981 agcggggcga taatacgtgc gacggtgtaa gcatctgttt gcgaactggc gagcgccgtc
    11041 agcaattgtt cgagaatttc tcgcgtggca cctggcggtg agggccagtt gagcaacata
    11101 cgtcgcaggc tggagatgac actggtcata cgtaattgct ggtggagtag cgcattaagg
    11161 cgcgcgtttt gctggcgaaa acgatagtgg ctccagaaag cctggatacg cagcaaattc
    11221 atggtcagta tctgcccaat ccccccctca tgtgctgcac gaatggcatc gtttgtttca
    11281 ggctgccaga gtaaactggc atgttccagt aatcgggcgt gcatgttttt caatgcggtt
    11341 aaaagggctg tagcatcgga actgctaggc aggatcatca tcatcatgcc gccgcacaaa
    11401 ataccgacta tcacctcgca aacgcgcgcc tgagcgatat cctacagctg gctggcctca
    11461 gtaatattaa ccatcggaaa ggcgatgatg gcagccgtgt agccgccagt tgaaatgcat
    11521 acgcgacgtt attcgtgaag tgcgcacagg cccaggtaca aaagccaagc cacgccgaca
    11581 tgctcaatag aaaaaaccac ggctcattga gcgtatgccc ggcaagaagc agtgccgcaa
    11641 tggctcccag taaactgcca gcgatgcgtc cgaggctttt gctgatgaca ccgccaacgg
    11701 tgggaaagct aaccactgca gccgaggtca tcgcccaata gggttcatcc agattcaaat
    11761 aataggcaac cgtcagcgcc agacacatgg caatggtatt gcgtaacgca taacgccatt
    11821 gcgccagcgt ggccttgaac cagggcaaat tgcgcaagga ccatgacgat gcgttcatta
    11881 ttgacctacg gcaatgctac aggtggtgcc cgacaccagc gtgacatcgc ctggaacttt
    11941 atccagcgca aagcggacgg gaacgcgttg ggcgagacga acccagggga cgttaggttt
    12001 cacatccgga ataaggcttg agtcactttc aacgctttga tcataaatcg cccgaccgat
    12061 actggaaacg tgaccccgta acgttttgtt atcactatag agggtaattt gtgctggtgc
    12121 accttcgcgg atatggcgca acttggtttc ttcaaaataa ccaatgacgt aaaacgagtg
    12181 gctgtcgaca agggcaaaca gcggtttccc ggtgtcagcg taatcgccga tgcgggtagt
    12241 gaggttagtc acccaaccac tcaccggggc gcgaatttcc gtttgcgcca gttgccattg
    12301 tgtctgtttc agcgtggcct gtgcggcatt aacactggcc tgcatcgctt taacattgag
    12361 gttagcggta tcgagttctt cggcagaaat aaaattttgt gagagatgac gacgacgatt
    12421 cgcttcgtta ttggcttttg cgaggtcaga ttgcgctttc gccagctgag cctgtgcgtt
    12481 cagctcggcg atctgaaagg gcgttttatc gatcgtgagc agaagatccc ccgcgttaac
    12541 cagttggttg tcttttatat tcagctcaac aatacgtccc gacacctgtg gggtgataga
    12601 aaccttttct gcgcgtattt tcccgtcgcg tgtccacgga gattgcatgt aataattcca
    12661 cagccaccat ccggcaagaa ccgcaactat cgctacaatg attattgaga aatacttaat
    12721 tgttttaatt gacatagtta ccacgcaatc agtattgcca gagcaaggca aacgcaaatc
    12781 gcaaacagcg ataaatccat ttacaaggga tgccagatgt caccggcgta gatccagcca
    12841 cgaagcaggc ggtgtacgac aagccagatg acgaatccaa acgcgaaggc tttgaaaaac
    12901 ggaggaaaat agacggacgc accgaacacc agatcttgta agggcaatcc tgttgcattg
    12961 agcataaact tcacaaagta gattccttta cgaccgaata tattgcgtta gattaataaa
    13021 tattctttaa gtgcgaaaaa tttacgcgca atttctgaaa aatatgtctg acggtaacca
    13081 aatgcagcaa tacatttgtt ttagcaataa aattgctgca cactattcta aaagccgcat
    13141 aatatcttag caagctaatt ataaggagat gaaattggaa tcgccactag gttctgatct
    13201 ggcacggttg gtgcgcatat ggcgtgctct gatagaccat cgcctgaaac cgctggagtt
    13261 aacacaaacc cattgggtta cgttacacaa tatccatcag ttacctccag accagtcgca
    13321 aattcaactg gcaaaagcga ttggcatcga gcagccatca ctggtccgta ctctggacca
    13381 actggaagaa aaagggttaa tttcgcgtca aacttgtgcc agcgatcgtc gggctaaacg
    13441 tattaaactg acggaaaagg cagagccact gatcagcgaa atggaagctg ttattaacaa
    13501 aacccgcgcg gaaatattac atggcatctc cgcagaggaa ctggagcaac tgattacgct
    13561 catcgcaaaa cttgagcata atatcattga gttacaggcc aaagggtgaa atgaaggggg
    13621 cgtgtggtca gtaacctgac cacacgcaaa cttatttagc gcggagaaac ggtcacctga
    13681 ctgccattgc tggccagtac gacacgttgg cccggagaga aacgagtgtt gccttgtttc
    13741 tgtaccacca tgatggtatt accatcgtct ttacgaattt ccagctcgac accctgcgtt
    13801 ttgttcattg cactctgtac gccctgacca gctacgccac ctgcaacagc gcctgctgca
    13861 gtagccagag aacgcccggt tccgccacca acagtattcc ccaggaaacc accaagaaca
    13921 gcaccgccaa ttgcaccgat aacgttggaa tcatcaccgc cctgaatctg taccggacgt
    13981 acgttaacga tggtgccata gctgacattc tgtacttgtt tcgcttcaga agcggtataa
    14041 acatcccctg acagggtgtc gttattaaca caaccgacaa gagacagacc taccattgaa
    14101 acaaccaata cgcgtttaat cattgaaaaa tctcctgttc accatgaaac gccacgcaag
    14161 catccctcat ggtcaaagta tatggcatat gtgaatccat ggttacatgt tctaccaaat
    14221 atgagcaaat cataatgaaa gtaggatcaa ccaattataa acaaaacatt catgatgatt
    14281 attgatttga agataatgtt ttactaccaa cggtaaaaat cggtcattcc gcatggtatt
    14341 cacgaaaagt ttatgtttga gtggtgcgca aacgtcagat tgaaaaagga tgaactatga
    14401 aatcgggccg ctttattggc gttatgtcag gcaccagcct tgatggtgtt gatgttgtgt
    14461 tggcgacaat agatgaacac cgggtcgcac agctggcaag cttgagctgg ccgatcccgg
    14521 tgtctctgaa acaggctgta ctggatattt gccagggcca gcagcttaca ctttcgcagt
    14581 ttggacagct tgatactcaa ctcgggcgac tttttgctga tgcggtcaat gccttgctta
    14641 aggaacaaaa cctgcaggcg agagatatag ttgcgatcgg ctgtcacggt caaaccgtct
    14701 ggcatgaacc gacgggcgtg gcaccacaca ctttacagat tggcgataac aatcaaattg
    14761 tggcacgcac cggaattacg gttgtcggtg attttcgccg tcgcgatatt gccttgggag
    14821 gacaaggcgc accgctggta cctgcgttcc atcatgcctt gctggctcac ccaaccgagc
    14881 gacgaatggt gctcaatatt ggcggcatcg ccaatctgtc actgctcatt cctgggcagc
    14941 cggttggcgg ctacgatacc ggtcctggta acatgctgat ggatgcctgg atctggcgtc
    15001 aggccggtaa accttacgat aaagatgccg agtgggcacg ggcgggtaaa gttattcgcc
    15061 cactgctgca aaatatgctc agcgacccat atttctcgca acctgcaccg aaaagcaccg
    15121 gacgcgaata ctttaactac ggctggctgg agcgccattt acgccatttt ccgggtgttg
    15181 atccccgtga tgttcaggcc acactggcag aactcaccgc cgtgaccatt tctgaacaag
    15241 ttttgttgag cggtggctgc gaacgattga tggtatgtgg tggaggtagt cgtaatccgc
    15301 tactcatggc gcgtctggcg gcattactgc caggcacaga agtcaccacc accgatgccg
    15361 ttggcattag tggcgatgac atggaagcat tggctttcgc ctggcttgcc tggcggacgc
    15421 tggcgggatt accaggaaat ctgccttccg tcactggcgc aagccaggag acggtactgg
    15481 gggctatttt ccctgctaac ccgtgatatc agagttaact gaatttttct taccgcgtct
    15541 gccgttacac ttggaacgtt agggagggcg tattgccctc cagaccagga aagtcttcgg
    15601 gaaatgtcta tgaaaaaact gttactgatc tgtttgccgg tgcttctcac cggctgtagc
    15661 gcgttcaatc aactggttga gcacatgcaa accgctacgc tggaatacca gtgtgatgaa
    15721 aaaccgttga cggtcaaact gaataatccg cgccaggaag tcagttttgt ttacgataat
    15781 caactactgc atctcaaaca gggcatttca gcgtctggtg cacgttacac tgacggaatc
    15841 tatgttttct ggtcgaaagg cgatgaagcg actgtctata aacgcgaccg catcgtattg
    15901 aataactgtc agttacaaaa tccacagcgt tgagattttt ccaggggcgg cgcacaatag
    15961 cgtcacccac tgacaatccg taaagaaaac catgtctgat aacgacgaat tgcagcaaat
    16021 cgcgcatctg cgccgtgaat acaccaaagg cgggttactc cgtcgcgatc ttcccgccga
    16081 tccattaacc ctttttgaac gttggctctc tcaggcgtgt gaagccaaac tggcagaccc
    16141 taccgcgatg gtggtcgcta ccgtggatga acatgctcag ccttatcaac gcatcgtttt
    16201 actcaaacat tacgacgaaa aaggcatggt gttttacacc aacctcggta gccgtaaagc
    16261 acatcaaatt gaaaataatc cgcgcgttag cctgctgttc ccgtggcata cccttgaacg
    16321 ccaggtgatg gtgatcggta aagcagaacg actttcgact ctcgaagtga tgaaatattt
    16381 tcatagccgc ccgcgtgata gccagattgg tgcatgggtt tcgaagcagt ccagtcgcat
    16441 ttctgcccgc ggtatccttg aaagtaaatt cctggagctg aagcagaagt ttcaacaggg
    16501 cgaagtgcca ttgccgagct tttggggcgg ttttcgcgtc agccttgaac agattgagtt
    16561 ctggcagggc ggtgagcatc gcctgcatga ccgctttttg taccagcgag aaaatgatgc
    16621 gtggaagatt gatcgtcttg caccctgaaa agatgcaaaa atcttgcttt aatcgctggt
    16681 actcctgatt ctggcacttt attctatgtc tctttcgcat ctggcgaaaa gtcgtgtacc
    16741 ggcaaaggtg cagtcgttat atacatggag attttgatgg caagcagtaa cttgattaaa
    16801 caattgcaag agcgggggct ggtagcccag gtgacggacg aggaagcgtt agcagagcga
    16861 ctggcgcaag gcccgatcgc gctctattgc ggcttcgatc ctaccgctga cagcttgcat
    16921 ttggggcatc ttgttccatt gttatgcctg aaacgcttcc aacaggcggg ccacaagccg
    16981 gttgcgctgg taggcggcgc gacgggtctg attggcgacc cgagcttcaa agctgccgag
    17041 cgtaagctga acaccgaaga aactgttcag gagtgggtgg acaaaatccg taagcaggtt
    17101 gccccgttcc tcgatttcga ctgtggagaa aactctgcta tcgcggcgaa caactatgac
    17161 tggttcggca atatgaatgt gctgaccttc ctgcgcgata ttggcaaaca cttctccgtt
    17221 aaccagatga tcaacaaaga agcggttaag cagcgtctca accgtgaaga tcaggggatt
    17281 tcgttcactg agttttccta caacctgttg cagggttatg acttcgcctg tctgaacaaa
    17341 cagtacggtg tggtgctgca aattggtggt tctgaccagt ggggtaacat cacttctggt
    17401 atcgacctga cccgtcgtct gcatcagaat caggtgtttg gcctgaccgt tccgctgatc
    17461 actaaagcag atggcaccaa atttggtaaa actgaaggcg gcgcagtctg gttggatccg
    17521 aagaaaacca gcccgtacaa attctaccag ttctggatca acactgcgga tgccgacgtt
    17581 taccgcttcc tgaagttctt cacctttatg agcattgaag agatcaacgc cctggaagaa
    17641 gaagataaaa acagcggtaa agcaccgcgc gcccagtatg tactggcgga gcaggtgact
    17701 cgtctggttc acggtgaaga aggtttacag gcggcaaaac gtattaccga atgcctgttc
    17761 agcggttctt tgagtgcgct gagtgaagcg gacttcgaac agctggcgca ggacggcgta
    17821 ccgatggttg agatggaaaa gggcgcagac ctgatgcagg cgctggtcga ttctgaactg
    17881 caaccttccc gtggtcaggc gcgtaaaact atcgcctcca atgccatcac cattaacggt
    17941 gaaaaacagt ccgatcctga atacttcttt aaagaagaag atcgtctgtt tggtcgtttt
    18001 accttactgc gtcgcggcaa aaagaattac tgtctgattt gctggaaata atgcattaag
    18061 tggaaagggg gagtgagaaa tcactccccc tggtttttat acagggaaca tgatgaaaaa
    18121 tattctcgct atccagtctc acgttgttta tggtcatgcg ggtaacagtg cggcagagtt
    18181 tcctatgcgc cgcctgggcg cgaacgtctg gccgctgaac accgttcaat tttctaatca
    18241 cacccagtac ggcaaatgga ctggctgcgt gatgccgccc agccatttaa ccgaaattgt
    18301 gcaaggcatt gccgccattg ataaattaca cacctgtgat gccgtgttaa gtggctatct
    18361 gggatcggcg gagcagggtg aacatatcct cggtatcgtc cgtcaggtga aagccgcgaa
    18421 tccgcaggcg aaatattttt gcgatccggt aatgggtcat ccggaaaaag gctgtatcgt
    18481 tgcaccgggt gtcgcagagt ttcatgtgcg gcacggtttg cctgccagcg atatcattgc
    18541 gccgaatctg gttgagctgg aaatactctg tgagcatgcg gtaaataacg tcgaagaagc
    18601 ggttctggca gcgcgcgaac tcattgcgca agggccacaa attgtgttgg ttaaacacct
    18661 ggcgcgagct ggctacagcc gtgaccgttt tgaaatgctg ctggtcaccg ccgatgaagc
    18721 ctggcatatc agccgtccgc tggtggattt tggtatgcgc cagccggtag gtgttggtga
    18781 tgtgacgagc ggtttactgc tggtgaaact gcttcagggg gcaacgctgc aggaggcgct
    18841 ggaacatgtg accgctgcag tctacgaaat catggtgacc accaaagcaa tgcaggaata
    18901 tgagctgcaa gtggtggctg ctcaggatcg tattgccaaa ccagaacatt acttcagcgc
    18961 aacaaagctc tgaaatatct catttcggcc cacaacgacc tgtgggccga tggctattac
    19021 tttaagcctt ccgctgacag tgcgtcttgt acttccggac gttcagccat acgttgcata
    19081 aatgctgcaa tgtgctctaa cccttccaga ttcagtttca ccgcgtatgc ccagcgcaga
    19141 accgtaaaca gataggcatc agcaattgta aatctttgcc cgcagatcca gtgctcatcc
    19201 ttcagtgtct cgttcacata ttgcagcttc ttctccagct gcgcgcgaac tgtcggtttg
    19261 tactcttccg gtgtatcagg gcgaaacaga ggtgtgaaac ctttatgcag ctcggtggcg
    19321 atgtaattca gccattcgat ggttttatag cgggaaatac tgtttaccgg tgccagcaac
    19381 tggcggtcgg ggacgctgtc ggcaagatac tgcataatcg ctacgccttc cgtcagcaaa
    19441 gtaccgtcat ccagcagcaa tgcaggcacc tgccccttag ggttaacggc aaagtaatcg
    19501 tcaccgtttt cgagacgttt tttcattaaa tccacactga cgagggtaaa atcctttccg
    19561 ctctcacgca gggtgatatg ggaagcgaga gagcaggcac ccggtttgta gaacaatttc
    19621 atcggtaact ccttttgtct gtggtttaaa gtatggtagt gcgttgctga acaaaaaaaa
    19681 agccgctaac gaaaagttag cggcttgtaa aagagtttcc ctaaatttta cgctacagca
    19741 gctttcgccg ctttgtctgc agcgtcatcc tgcgtcatac ggtgcagttt aggcgcggtc
    19801 agcagcatca gtactgcaat aatggcagta gcgacaccaa tctgcaagaa tacgcgacca
    19861 taaacttcca gtgacatcag cgggtcggta acgttatccg gcacagccat catacccgca
    19921 acataaccac caatcaggtt tgcaccggca gtggtcagga accagctacc cataatgaag
    19981 cccatcagac gctgcggaac gagttgtgca accattgcca gacccagacc agagatcatc
    20041 agttccccga tgctctgcag gccatagctt gcgaccagcc agcttacaga cacgatacca
    20101 gcgtcagacg cgaatttcgc acccagcggc agaatcagga acgcaccaga acacatcacc
    20161 atgccgattg caaacttggt tggcatcggc agggtatcgc ccatcttgtt atagatagcg
    20221 gccagaatcg gactaccgat gatgatccag aacgggttca gtgcctgata ctgctcaggt
    20281 tctacggcca gacccagaat ggagtgctca acgttacgaa tcgcaaagaa gttcagtgac
    20341 gttggcatct ggctgtacag cacgaagaag ataatggctt cgagcatcag gatgaaggca
    20401 acgatcattt tacgacgcgc agcacctttc atcgcgaagg cttctttacc gaagataacc
    20461 acgataccga aggcaacaac gcccagcgcc atacgcgcaa cttcctgatt gtgcagcagc
    20521 caggtggcga tagcgatcag tgccacaaca ccaataatgg tcagtagcag gttacggtag
    20581 ttgataggct cgaagtctgg ttttgaaccg tactgtttaa cccagcgttg gcagaaggcg
    20641 aagttaacga tagtgatcag caggcctaca acgctcaacg caaacgcaac actccagccg
    20701 tatttcgcgg ccagccacgg cgtagcaatc atagagaaga aagagccgat gttgacggac
    20761 atgtagtaca tggtgaatgc accgtccaga cgcgggtcgt ttttctcata gcatgtagaa
    20821 agcagagaag acgggttagc tttaaacagg ccgttaccga ccgcaatagc cgccataccc
    20881 atataaacga taccggcgtc gtgaccagac caggcaacca gagcataacc aatcgccagc
    20941 acaatagcgc cgagcataat tacgcgttta gtacccagta ccttgtcacc taaccagccg
    21001 ccgatagcga ccagaccata aaccagggca ctaaaggaag agaaaagggt gattgaatcc
    21061 gcttcagaca tacccagttg tttaaccagg taaacagcca taattccttg taggccgtaa
    21121 taaccaaaac gttcccataa ctcaatcgag aagatgagat agaacgcctt cggttgtttg
    21181 aaagcgttca aactgacgct ttcagttggt ttttggtttg cagtggacac ttttacctct
    21241 ttttttacat cccatattaa cgggggtgct ttctcgcgtt atgtctaaaa aacatacgcg
    21301 caattgttat agtgggaggg gaaacggcag gtaatgttca ctatcttgca ctttcagaca
    21361 agtcctttgt aataatttgt tacatataac tgaggcgggg aattcaacca ttccgggaaa
    21421 tgttatccag cgttaaattt ttcaaaaaac gtaacggcag attttttcac tgcactgaga
    21481 ggggggtgat ggcttaacgg cgatatgttc tgctatttag cgtttttact agtgagatag
    21541 tccagtttct gaaaaatagc cagtgtaatg ttttgtaggt caatatttgt tgcgtgtgta
    21601 aatttaaatg attttaacct gattcttgca atgtttttac attgcgacag catcgtgatc
    21661 ttgatcacga atgaatagaa aatttcgcta ttaaaaaaac aattaacctg catttttggt
    21721 tatccgcaaa cggattacct gatgataacg ctaagcaatg gcatcattca cgttaaaaag
    21781 atgttttaaa agaggataaa gaaaggttat caatggggta atcggtgtta ccccttttct
    21841 tcagatgtca actttctctt tgtattcaca aagatcttca ataatacaag agccacagcg
    21901 gggcttgcgg gcaatgcagg tataacgccc gtgcaggatc aaccaatggt ggcagtcgac
    21961 tttaaactct gccggaacca ctttcagtag cttttcttct acctgttcaa cgtttttccc
    22021 cggcgcaaat tgagtacgat tacaaacgcg gaaaatgtgc gtgtcgacag caatagtcgg
    22081 ccagccgaat gcagtgttta atacgacgtt ggctgtttta cgacctacgc cgggcagggc
    22141 ttcaagcgca gcacgatctt ccggaacctc gccattatgc tgctccagta agatacggca
    22201 ggttttgatg atattttctg ctttgctgtt ataaagccca atcgttttga tataggtttt
    22261 aaccccttca acgcccagtt caagcatcgc tgcaggcgta ttcgccaccg ggtagagttt
    22321 cgccgtcgcc ttattaacac tgacatcggt cgcctgagcg gaaagcagta cggcaatcag
    22381 caattcaaaa ggcgaactga aattaagctc ggtggtggga tgaggattgt tctcacgcag
    22441 gcgagtgagg atctccaggc gttttgcttt gttcatcaga cattccctgt ttcaccgttt
    22501 ggcaatgcac gttcggcagc tgcttcagta cggcgctttt tcattctttc atcaatcagg
    22561 tattttcctg ccagcatcag tcccaggcca ataaatgcac ctggtggcag catcgccagc
    22621 aggaaagggg agtcggtgtg gaaaatctcc acgcgtaata cttttgccca gctacctaac
    22681 agcgcatctg caccgtcaaa caatgtgcca ttgccgataa tttcgcgtag tgaacccagc
    22741 acgaacatgg cgcaggttgc gcccatacca attgaaaagc cgtccagtgc cgaaagcgcc
    22801 ggaccttttt tggcggcgaa ggcttcagcg cggcccacaa cgatacagtt agtgacaatc
    22861 agcggaataa aaatccctaa tgattgatac aggccaaagg cgtaggcgtt gatcagcatc
    22921 tgtacagcgc tgaccaccga ggcgatgatc atcacgtaaa tgggaatgcg gatctcggct
    22981 ggcgtccagt gacgcagcgt cgaaatggtc aggttggtca gcgtcagtac cagcgtagtc
    23041 gcaagtccta aacccagagc attagtggca gtggacgtga ccgccaacag aggacaaagg
    23101 ccgagcaact ggaccagcgc agagttgttt ttccacaacc cctgaacaat aacgtcttta
    23161 atttcgctca cgctttattc tccacaggca ggaagttgag aaagttgtgc cggtaacgtc
    23221 tgagcgtaca atccggcgcg ttttaccgca ttaaccaccg cgcggggagt aatcgtcgcg
    23281 ccggtgaact ggtcgaaatc accaccatct ttcttcaccg cccagtgcgc atcatccgca
    23341 ccactgattt ttttacccgc aaaatgggtg atccagtcag aaaggcgcag ttcgatttta
    23401 tcgccaagcc ctggcgtttc gtggtgctct gtcacgcgcg tgccaagtac cgtgccgtta
    23461 aaatcggctc ccaccagcag ctgaatcgca ccggaatagc catctggcgc ggttgcttcc
    23521 agaacggcgg ctaccggttt gtcatcctgt ttggcgatgt aaacccgatg ctcaccttta
    23581 cctaactctg gcgcagttac cagatagcaa ctctgtgcca gcgcattgtt atagcgttcg
    23641 gctggcagca cctgatcaaa taatgccttt tgttgcagac ttgcctgttc agcaatcgtc
    23701 gttttggtca tctggttgat ggccgcagtt aaccctgttg aacccgctgc aaatagcgct
    23761 aacgtaatgc cgtgttttcg gatagttttc agcatggttt accctttgcg atggccgtag
    23821 acgcgcggac gcgtgtagta atcgatcaga ggaaccgtga tgttcgccag caggacggca
    23881 aaagccacgc cgtcaggata gccgccgaaa ctgcggatca accagactaa taagcccgca
    23941 agcgcgccga aaataagacg accacgattg gtcgtagaag cggtaaccgg gtcagtcaaa
    24001 ataaagaatg cgccgagcat ggtcgcacca gacagcagat gaatttgcgg tgctgccagt
    24061 gtttctggtg agaacaacca gcccaacgtt gcgcataacg ccagcgttac taagaagctg
    24121 aggggaatat gccagcgaat cgctttctgc catagcaacc acaggccgcc agccagccag
    24181 gcgagattta cccattgcca accagcgccc gccagaatac cgctgtagat cggatattgc
    24241 ataatctgtt caaccgaatg accggcacgg acagaggttt taaatgtatc cagcggtgtc
    24301 gcctgactaa tgccatcaat acctaagtgt agtgtgttca tatcagcacc actggcggta
    24361 tgcccgctga aaataacctg tatggcgtcg ataaaaccag ggatgttgac cgcaatttca
    24421 tgtggcggta accagctggt catctgcacc gggaaggaga tcagtaagac cacataacca
    24481 atcattgccg gattaaacgg gttttgcccc agaccgccat acaactgttt agcgatgatc
    24541 accgcaaaca ccgtacccag cacgaccatc caccatggcg cgaggggggg aatacttacc
    24601 gccagcaata agcctgtcag caatgctgag ttatctttca acgttgcggc taccgactgc
    24661 ttgcgtagtt tgagtacgag agcttcggct aacagagtac taaccgatgc caacaggatc
    24721 tgaacgagag taccccaacc aaaaaaccac agttgcgctg cgattcctgg cacggctgcg
    24781 agcaacacca acagcataat gcgcgatgtc tggcgctggt tatgggtata aggggagcta
    24841 gctattctga ataccattta gtcctcgttt acaacctttt gctgggcggc ttttttggcc
    24901 tgagcgcggg caatagccgc ggcaactgcc gctttgcgcg gatcaacctg ttcttctggt
    24961 tctgcgttag tctgttgctg ttccagcttg cgcgctttgg cacgggcaat agcggcttcg
    25021 acggcggctt tgcgcggatc aacctgttct tcaggtaccg cattagcctg ttgctgttcc
    25081 agcttgcgtg ctttggcgcg tgcaatagcc gcttcgacgg cagctttgcg cggatcaacc
    25141 tgttcttctg gctccgcatt agcctgttgc tgttccagtt tgcgcgcttt ggctcgggca
    25201 atagcggctt cgatggcggc tttgcgcgga tcaacctgtt cttctggttc cgcattagcc
    25261 tgttgctgtt ccagcttgcg cgctttggca cgggcaatag cggcttcgac ggcggctttg
    25321 cgcggatcga cctgttcttc tggttccgca ttagcctgtt gctgttccag cttgcgcgct
    25381 ttggcgcggg cgatagctgc ttcaacggca gttttacgtg gatcagcaac ggttgctgcg
    25441 tcgttagttt gctgcagttc tgcctgtttc gctctggctt gcgctttacg ggcttcccgt
    25501 gctgcaataa ttgcactgtt atccgggcgt tcgcccgctt taatcacaat aggctgtgta
    25561 gcctgggcct gtttctcttt cacccgcgcc agagcggcag caatcgcatc tttatctttg
    25621 gctgcaggtt gaacggctgc gctcttatgt cgttcaaggc gagccgcttt ttcgcgctcc
    25681 agacgagcct ggcgcgcttc gaaacgcgct ttggcttctg cggcgcgctt ttcttcctga
    25741 cgaatagccg caatttcagc tttttcctga cggaaatatt gcaccagggg aatattgctc
    25801 gggcaaaccc acgcgcaagc cccacattca atgcaatcag caatgttatg cgtggtagct
    25861 ttatcgtgtt gctgaccttt gctgaaccag tacaactgtt gcggcaaaag atccgcaggg
    25921 caggcgtcag cacaggcgct acaccggatg cagctttgtt cttcctgtgg ttcgccaagt
    25981 tcattggcag agggagccaa cagacagttg gtaatcttta cgaccgggac atccagccat
    26041 ggcaaggtaa agcccattag cgggccaccc ataatcacca tttgatcggc agaggggcag
    26101 aatcgggcat cattcaataa atgacgcact ggcgtcccca gccgtgccca gacgttgccc
    26161 gggcgagcga ttgcttcgcc agtcagggtt acaacacgct cggtaatcgg ctcgccatca
    26221 ataacggcac gtttcactgc ataagcagtg ccgacgtttt gcattaatac gccgatatcg
    26281 gatgaacgcc cgccatgtgg aacctgcttc ccggtcagaa tgtaggttaa ttgtttagca
    26341 ccgccagaag gatatttggt tggaatcacc cgcagagaaa tatcgttaga gtccgccagc
    26401 accgcgcgca gcatggaaat cgcctgcggt ttgttatctt caatgccgat aagaatttcg
    26461 cgtggctgca gaatatgcgc aagaatgcga ataccctcta cgacctgagc cgcgcaatcc
    26521 tgcatcaaac ggtcatcggc ggtaatgtac ggctcgcact cagccgcgtt gataatcaac
    26581 gtttcaatct tatctccgcc accctgcaat ttaacgcctg tcgggaatcc tgcaccgccc
    26641 agcccggcaa caccaaactg atgtatgcgc tcgattaact cttcgcgact acgagtgcga
    26701 taatcggccc agccgtcgcg cgggatccag cagtcttcac catcggcatc aataatcacg
    26761 cttaattcag ctaaagctga aggatgagcc gtagagtggg gcgcaatagc cgtaacggta
    26821 cccgaggtgg gcgcgtgaac aggcagcatt ttgccgcgac cacgggtaag cggctggccg
    26881 cgcaatactt tatcgccgac gctaacgcac aactcacctt cagcgccaat atgctgtttc
    26941 agtggaataa caaaacgctg cgccagtggt acctggcgca ggggtgtacc gttggactgg
    27001 gttttcatct ccggtggatg gatgccgccg ttgaaatccc agattttatt ttttctgaat
    27061 gcagagaata acttaagcat ggtgttccac gggaatgata cgcacgggaa tggtgttcag
    27121 atcccatttc caggagtcag gtgtttctgc gaccggttgc aacgagatgc agtgcgtcgg
    27181 gcacggatca acacataaat tgcagcccgt acagagatca ctcattaccg tatgcatggc
    27241 acgggtagcg ccaacgatgg cgtctaccgg acaagcctga atacatttag tgcagccaat
    27301 acagttattt tcatcaataa ccgccaccat ccgcgcaggc gttagctctt gcgcttcgcc
    27361 atccagcggc tgcggctcga cattaagcaa ctcggcaatt tttagcatca cagcttcgcc
    27421 acctggggcg caacggttga ttttttcacc gttacagctg atggcttccg cgtaggggcg
    27481 acagccggga taaccgcact gaccacactg gctctgcggt aagatttcgt caattttctc
    27541 aacgaccgga tcgtcttcca ccgcaaaacg gcgggaggca taacccagaa tggcgccaaa
    27601 cgccaggccc agcaggctca cggcggcaac ggcaatccag atagcattca ttacaacttc
    27661 accaaaccac taaagcccat aaaggccaga gacataagac ctgcggtaat taacgcaatg
    27721 gcattaccgc gaaaaggtgc cgggacatca gccacagcaa ggcgttcgcg gatggcggtg
    27781 aagagcacca tcaccagcga gaaaccgaca gcggcggaaa aaccgtacag cgccgactgc
    27841 aagaaattgt gcccgagatt gatattcagc aacgccacgc cgagcactgc acagttggtg
    27901 gtgataagcg gcaaaaaaat ccccagcaga cggtaaagca ccgggctggt tttgcgcacc
    27961 accatctcgg tgaactgcac gaccacagca atcaccagaa taaatgccag ggtgcgcagg
    28021 taaatcagat taagtgggat caaaatccac gtatcgataa gccaggcgca aatagacgcc
    28081 agcgtcatca caaacgttgt tgccagcccc atgcccatcg cggtttccag ttttttggaa
    28141 acccccataa acggacagag accgagaaac ttgaccagca caaagttatt gaccagtaca
    28201 gttccgacaa acagtaacag gtagtcagtc atttttcggc ctgaaataaa aaagccgcct
    28261 attatccgtt aatccagagc aggcgacaac aggttaactg taaggttatt acggattcac
    28321 aaacgtcgct tttacccgcg acgagcgttt gaaataggag acgatcaatg cggttgccaa
    28381 caaagtaaac agcaactggc gaacggctat gccatcttcc acgggtgaaa aagcgaatgc
    28441 ttttactgcc agcaatacgg aaataagcag ccagataata tagtgtttag gaacacaacg
    28501 ccgacgttta aagaaagcga tggtcaacca cagtgtgtaa taccacatgg cgatagcggt
    28561 aataaaagaa acgccccata agatttgtgt cgtcagtgct tgtccgccga gcgtttgaaa
    28621 tgtttgcgga gaagataacg cagcggtata cagcaacaac gccagcgtcg tactcaacag
    28681 agcgactaaa agccaggcca acgggccaag caaccagcct ccaatacgtt gtggcgttgt
    28741 ggtggtcata ggttctccag aatagcgacg caaacaggca aatctaattt taggggcgtt
    28801 attataaggc ttttataacc gatcgctact ttctttattg gacatagtgc cagacggact
    28861 tcggtacctg gccgaggtca aacaaacgcc cgccagaaac cagctctgcg cgacggtgat
    28921 cggcagcacg atacatattg atcagttcct gatcgtccgt cagggtgtaa ttaagatggt
    28981 cgtagagttt ttccagactt tcgagtgaac tgattttgcg aaattttaat aagtagtcct
    29041 gaacagtcat aaaaggtcca taaaataatt aataccaaag gggtaggtat tattagtgat
    29101 gataaaattt caccaaaagt gaacgcttgt gttattacaa taataagaat gcgctgatgg
    29161 caaatattca gggatgaaaa actgctctga aaagagtaac gcgctttact atccctgaca
    29221 atcaccaaca acatcgaaca agataataaa ttcctggttt aatatccgac aagtgaaaac
    29281 atgcaaccgg acgggcagca tggcgctcta caagcgcaga gcttacttgt gttgtaccga
    29341 agcactctgt tcaggtggct gatagttgtc aatgtgactc gccacgccaa gaagaatgac
    29401 tgaaacgaca agaacgatcc aacctgttaa ttcaataaga cgattcatta cagcccacat
    29461 ctctcttgat tgatccatta acttcagggg gtaaatgtta cttagcaata atagctccag
    29521 caagattttt actgaggttt ttgcgatatt tagcttttgt cgttggaaaa ttcgctattt
    29581 ttttgactta atattaaact gttgagcgtg ggtaaaggat agtgtcaaat agcaatcata
    29641 cttcaatgag aggcaatgac atgagcgaca acatccgtgt tgggttgatt gggtatggtt
    29701 atgcgagcaa aaccttccat gcgcccctga ttgcgggcac gcccgggctg gaactggcgg
    29761 taatctccag cagtgatgaa acaaaagtaa aagccgactg gccaacggtt acggttgtct
    29821 ctgagccgaa gcatctgttt aacgatccca acatagacct gattgtcatt cctacaccca
    29881 acgataccca tttcccgtta gccaaagcgg cgcttgaggc gggtaaacat gtggtcgttg
    29941 ataaaccctt taccgtgaca ctgtcacaag cgcgagagct ggatgcgctg gcaaaaagcc
    30001 tggggcgtgt gctgtctgta ttccataacc gtcgttggga tagcgatttc ttgacgctaa
    30061 aaggtttgct cgcggaaggc gtgctgggtg aagttgctta ctttgagtct cattttgacc
    30121 gcttccgtcc gcaggtgcgc gatcgttggc gtgaacaggg cggcccaggc agcggtatct
    30181 ggtacgattt agcaccgcat cttcttgatc aggccattac gctatttggt ttaccggtca
    30241 gcatgacggt agatttggca cagttacggc ccggagcgca gtcgaccgat tatttccacg
    30301 ccatcttgtc ctatccacag cggcgagtca ttttacacgg taccatgctg gcagccgctg
    30361 agtcagcacg gtatatcgtg catggatccc gaggcagtta tgtgaaatat ggcctcgatc
    30421 cacaggaaga acgtctgaaa aatggcgagc gtctgccgca ggaagactgg ggctacgata
    30481 tgcgtgatgg cgtacttacc cgcgtggaag gtgaggaacg tgtcgaagaa acgctgttga
    30541 cggtacctgg gaattatccg gcttactatg cggctattcg tgatgcgtta aatggcgatg
    30601 gtgaaaatcc ggttccggca agccaggcaa tccaggtaat ggagttgatt gagctgggca
    30661 tcgaatccgc caaacatcgc gcgactttgt gccttgcatg aaaaatggat ggtgcaattt
    30721 gcaccatcca ttttgttact tcgcggcgac tttttctcgc agtgcgcgtt tttcctcagc
    30781 gctgaggaaa gccatttcca gaccattaat ctgtgcctgg cggatttgct cgcgggataa
    30841 cccagcagct ggcgcggcaa cggtatattc gtgaatgata tccactccct gtacgccggg
    30901 atcgtcagtg ttaatgccgg cacgaatgcc atgctcaagg aacgttttca gcggatgtgc
    30961 agccagctct gctacggtgc tggtctgaat attggaggtc agacaggatt caataccaat
    31021 ttgttgctcg gcgagaaaat ccatcagcgc ccgatcttca atggctttta cgccatgtcc
    31081 aatacgctcc gcacccagtt cacgaatcgc ctgccagatg ctttccggcc cggcagcttc
    31141 gcctgcatgg acggtaatat gccagcccgc atcacgcgca aggttgaagt gagaaaggaa
    31201 cagacttccc gggaaaccaa gttcatcacc ggctaaatca agggcggtaa tctggtcacg
    31261 gtgggctaaa aaggcctcca gctcttgctg acaggcggct tcgccgaagg ttcggctcat
    31321 aatgccgata agcttcgcct gcacaccaaa agtgcggcaa ccttcacgta cgccatcgat
    31381 caccgcttcg acaacacccg ctacaggcag cttatgtgcc attgccatgt agcctggtga
    31441 aaaacgcagc tcgacatagt gcaggccgtt acgggctgca tcttcaatgt tttcaaatgc
    31501 cacgcggcgg caggcatcaa gagaggcgag aactttaacg ccccagtcaa gtttggtcag
    31561 aaagctcacc agatcgggtt cgttggcaat gacctgaacg tggggaatca gtgtttccag
    31621 ggattgtgca ggaagcgaga tattatactg gcggccaagt tcaagaatgg tctggggacg
    31681 aatgttgcca tcaaggtggc gatggatatc agttaatggc agggtggtat caatcatggt
    31741 cgcactcttt ttctggatat aaaagtgcgc accattataa aaacaaagca gggtaaaaat
    31801 ccacgattgc gcaacgattt ttacgttgcg caattggggt taacgaacag cgcggatggc
    31861 gttaattaat ccagccacac ctttttccag tttcgaacgt gggcagccgg cattgagacg
    31921 gacaaaacca cgaccttctt caccgtaggt ataccccggc atgatcgcga ctttttcttg
    31981 ttcgataagt gctttttgca acgcgttgtc gtcaatattc aacggacgta aatcaagcca
    32041 tgccagataa gtggattgtg ggatctgcca gttgagttca ggaaacgcgg cgttcatttt
    32101 atctgcgata tacgtcaggt tatctttcag atagatgcgt aaggcatcca gccacggcgc
    32161 gccttgctga taggcggcga tatgggcagt taacgccagt accgaagggg aagaaagccc
    32221 atcacggcct ttcagtgccg ataaataggc atcgcggcta ctgctatttt ctataatccc
    32281 gtaagcaccg gtcagggcgg gaatattgaa acttttcgag cccgacgtta gcaacgccca
    32341 gtctccgcga gccacattac tccagggaat atgcggctgc tcgccccaaa ccatatccat
    32401 atggatttca tcggaaataa cccgcacacc atgacgctcg cacaggtcag ccatgatctc
    32461 cagctcatcg cacgtccaca ctttcccggt aggattctgt gggctacaca ggagcataat
    32521 tttacattct ggtttcgcca acacggcttc caacttgccc atatcgcaaa accaaccatc
    32581 agcctgcttc tctaaagcaa cgggcattac tgtgcgctgg ttaccttcaa tggccttgta
    32641 aaatgcgtca taggcgggtg tgtggatcac cacgccttca cctgtttcag accactgacg
    32701 aatcagttct gaaaccatat agatgacaga agggccatac accaccgtct gagaatcgat
    32761 ggcggtgtaa tgctgggtgg aaaaccagtg ggcaatagca gcgagaaact catcgttttt
    32821 ccagcggctg tagccaaata cgccgtgcat caggcgctga ttcagcgcct ctataatgca
    32881 gggggcagtg gcaaaatcca tgtctgaaat cgtgaacggt aacaggtcag cagtgccgaa
    32941 acggtcagcg acataatccc actgtgtaca ccatgtgcca tgacgatcca cgacctttga
    33001 aaaatcgaac atatcttatc cttatgcctg gacagtatgc atcagaccgg ccatttcatc
    33061 ttttactgac tgaacttgtg gcccgataac aacctgcagg ttatgttgat taagttgtac
    33121 tacgccaatt gcccgattgt ccttcagtgc ctgcacatta acaagcgaca tatctttcac
    33181 agacaaacgc agacgggtaa tgcagttatc gaggctgaca atattgtcgg caccgcctaa
    33241 tgcttcgagg attgcaggaa cgttgtaacc tgatttaccc ggcgcagcgg caacggcttt
    33301 ttcgattgag ctggcaactt cgctatcgcg ccccggggtt ttgagattga agcgggtgat
    33361 agcgaaacgg aagatggcgt agtaaacgac aaaccagatt gccgccacca ctggcaccat
    33421 gtaccacttg gttgacagac catgcaaaat accgaacacc acgaagtcga tgatattgcc
    33481 gtcggtatta ccgatggtga cgccgagcac agacatgacg gtgaagccga ggccggttaa
    33541 cagcgcgtgg atgacataca gaactggcgc tacgaacagg aacaggaatt ccagcggttc
    33601 ggtagtgccg ccaacgacgc aggcgatcag gccagaaatc agcagacctt taattttatg
    33661 gcgattttcc gggcgcgcgc agtgatacat agctaacgct gcagctggca gaccgccgag
    33721 aaacgcaggc attttacctt gcgaaaggaa acgcgtggcg ctttcagaaa aaccgtgagt
    33781 ggtcgggcaa ctcaattgcg tctggaagat ggtcagtgcg ccgctgacgg tttgaccgca
    33841 gacttcctgc gtgccgcctg cgtcggtaaa gcgaattaat gccaccagaa tgtgatgcag
    33901 accaaacggc aacagcagac gttcaccggt accaaacagc atcggtccga aatcacccgc
    33961 gctgtttatc atatggccca agccgctaat acccatggcg aaaatcggcc agactaatgg
    34021 aatcaccagg ccgacaaggc ccatcaccag cgaggagata attggtacga agcgcgtacc
    34081 gccgaagaat gccagcgcat ccggcaggcg gatattatgg aaacgctcat gcagcatcca
    34141 gacgataata ccggcgatca ccgcaccgag gatcccggta tcgatcgact ggatcccaag
    34201 aatgctctgg atgttattgg ctttcagaac cgcggcatcc gtggttggca gaatgccttt
    34261 attggtcaac cagaagttta ccgcgaggtt cattaccgca taaccgacga agccagcgaa
    34321 tgccgctacg cctttatttt cgcgtgccag gcccagcggg atggcgatac agaacatgac
    34381 aggcaggaaa ctaaaagcaa acgagccaat cttactcatc caggtaaaga tagcctgcaa
    34441 cacggggttg cccaggaccg ggatcagggt tatgacatca tggctgctaa gagaactacc
    34501 aatgccgagc ataatgccgc agaacgacaa taatgccacg ggtaacatga aggttttgcc
    34561 taactgctgg aagaactccc acagcgtgac tttcggtgct gttttcgccg tcataaaacg
    34621 actcctcgta gagaacaatc tggattcagt aaattgcgcg agaagataaa acgttttatc
    34681 aaattttagt gaggcataaa tcacattacg caacgataat agcgggtata agataaataa
    34741 aaggtaaaac gttttatctg tcacataatc agggagtagg tcatctgcat ggctaccgcc
    34801 aaaaaaataa ccattcatga tgttgcgctg gctgcgggcg tgtcggtaag taccgtttcg
    34861 ctggtgctta gtggcaaagg gcgaatctct accgccacag gagaacgcgt taacgccgcc
    34921 attgaagagc tgggatttgt gcgcaatcgc caggcgtcgg cgctgcgcgg cgggcaaagc
    34981 ggcgtcattg gtttgatcgt ccgtgattta tctgcgccgt tttacgccga attgacggcc
    35041 ggattgacgg aagctctgga agcgcaggga cggatggttt ttttgcttca cggcggtaaa
    35101 gacggcgagc agctggcaca gcggttttca ctgttactga atcagggggt cgatggtgtg
    35161 gtaattgccg gggctgcagg aagcagcgat gacctgcgac ggatggcaga agaaaaagct
    35221 atcccggtga ttttcgcttc ccgtgccagt tatcttgatg atgttgatac ggttcgcccg
    35281 gacaacatgc aggctgcaca gttgttgacg gagcatctca ttcgcaatgg gcatcagcgg
    35341 atcgcctggc tgggagggca aagttcctca ttaacccggg cagaacgggt gggaggctat
    35401 tgtgcaactc tactaaaatt tggtctgccg tttcacagcg attgggtgct ggagtgcaca
    35461 tccagccaga agcaagctgc ggaagctatc acggcgcttt tacgtcataa cccgaccatc
    35521 agtgctgtgg tttgctataa cgaaactatt gcgatggggg catggtttgg tttgctgaaa
    35581 gcagggcggc aaagcgggga aagcggagtc gatcgttact ttgagcaaca agtttcgctg
    35641 gcggcattta ccgatgcgac accaaccaca cttgatgata tacccgttac ctgggccagc
    35701 acgcctgcgc gggaacttgg taccacactt gcggatcgca tgatgcaaaa aatcacccat
    35761 gaagagacgc attcacgcaa tcttattatt cccgcccggc tcattgcagc gaaataatcc
    35821 tctctttatc tgctatacct ggtagtgtcc tttcctcaag gttaatgata aaaaatgtgg
    35881 cacacagg
//
LOCUS       DAJTMC010000022        35122 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.022, whole genome shotgun sequence.
ACCESSION   DAJTMC010000022 DAJTMC010000000
VERSION     DAJTMC010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35122
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.022"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            32..1270
                     /gene="dsdA"
                     /locus_tag="OO130_000962"
                     /pseudo
     CDS             32..1270
                     /gene="dsdA"
                     /locus_tag="OO130_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416867.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
     gene            complement(1378..2916)
                     /gene="emrY"
                     /locus_tag="OO130_000963"
     CDS             complement(1378..2916)
                     /gene="emrY"
                     /locus_tag="OO130_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HCR5364462.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRLLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(2916..4079)
                     /gene="emrK"
                     /locus_tag="OO130_000964"
     CDS             complement(2916..4079)
                     /gene="emrK"
                     /locus_tag="OO130_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HCR5364463.1"
                     /translation="MEQINSNKKHSNRRKYFSLLAVVLFIAFSGAYVYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLALKRTDIKSPVTGY
                     IAQRSVQVGETVSPGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKNEDIAEMPELASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            4495..5109
                     /gene="evgA"
                     /locus_tag="OO130_000965"
     CDS             4495..5109
                     /gene="evgA"
                     /locus_tag="OO130_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HCR5364464.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGASAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            5114..8707
                     /gene="evgS"
                     /locus_tag="OO130_000966"
     CDS             5114..8707
                     /gene="evgS"
                     /locus_tag="OO130_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HCR5364465.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQRVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALEEGEVDIVLSHLVASPPLNDDIAATKPLIITFPALVTTLHDSMRPLT
                     SSKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGQNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVVLNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPDLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLNPGGWDIIPGAIYSEDRENNVSFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIKVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDHAVYI
                     CGWQDITETRDLIHALEVERNKAINATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLV
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEHPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILITFQHETHKDLPAAFQALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPVSDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(8763..9908)
                     /gene="yfdE"
                     /locus_tag="OO130_000967"
     CDS             complement(8763..9908)
                     /gene="yfdE"
                     /locus_tag="OO130_000967"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HCR5364466.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDYDKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWETLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFNTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLARIHEVGVPVAPLLSVAEAIKLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(9982..10926)
                     /gene="yfdV"
                     /locus_tag="OO130_000968"
     CDS             complement(9982..10926)
                     /gene="yfdV"
                     /locus_tag="OO130_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HCR5364467.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(10996..12690)
                     /gene="oxc"
                     /locus_tag="OO130_000969"
     CDS             complement(10996..12690)
                     /gene="oxc"
                     /locus_tag="OO130_000969"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HCR5364468.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVIGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSSVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(12744..13994)
                     /gene="frc"
                     /locus_tag="OO130_000970"
     CDS             complement(12744..13994)
                     /gene="frc"
                     /locus_tag="OO130_000970"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HCR5364469.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(14507..15139)
                     /locus_tag="OO130_000971"
     CDS             complement(14507..15139)
                     /locus_tag="OO130_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HCR5364470.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQVQQTQKTAA
                     AERISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKT
                     NLNDDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVME
                     NQYLMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            15435..15710
                     /locus_tag="OO130_000972"
     CDS             15435..15710
                     /locus_tag="OO130_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5364471.1"
                     /translation="MKVNLILFSLFLLVYIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDAPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(15787..16029)
                     /locus_tag="OO130_000973"
     CDS             complement(15787..16029)
                     /locus_tag="OO130_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HCR5364472.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTEIFMFLAPACGLMIRFMVGYGRR"
     gene            16382..17302
                     /gene="lpxP"
                     /locus_tag="OO130_000974"
     CDS             16382..17302
                     /gene="lpxP"
                     /locus_tag="OO130_000974"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HCR5364473.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHCRFKTRPVGESSLYI"
     gene            17658..17729
                     /gene="ypdK"
                     /locus_tag="OO130_000975"
     CDS             17658..17729
                     /gene="ypdK"
                     /locus_tag="OO130_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HCR5364474.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(17794..19032)
                     /gene="alaC"
                     /locus_tag="OO130_000976"
     CDS             complement(17794..19032)
                     /gene="alaC"
                     /locus_tag="OO130_000976"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HCR5364475.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRQDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWIVEMPKASMYVWAKI
                     PEPYAAMGSLVFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
     gene            19409..21105
                     /gene="ypdA"
                     /locus_tag="OO130_000977"
                     /pseudo
     CDS             19409..21105
                     /gene="ypdA"
                     /locus_tag="OO130_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
     gene            21120..21854
                     /gene="ypdB"
                     /locus_tag="OO130_000978"
     CDS             21120..21854
                     /gene="ypdB"
                     /locus_tag="OO130_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HCR5364476.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            21867..22724
                     /locus_tag="OO130_000979"
     CDS             21867..22724
                     /locus_tag="OO130_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5364477.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNSQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYTQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(22727..25222)
                     /gene="ptsP"
                     /locus_tag="OO130_000980"
     CDS             complement(22727..25222)
                     /gene="ptsP"
                     /locus_tag="OO130_000980"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR5364478.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQAQRVLEEYIQVRFIDSDSIQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASSVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRESDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQDFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLAKPIDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(25247..26284)
                     /gene="ypdE"
                     /locus_tag="OO130_000981"
     CDS             complement(25247..26284)
                     /gene="ypdE"
                     /locus_tag="OO130_000981"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCR5364479.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGTRSYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHSAELPAEVWLVASSSEEVGVRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQICNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTVVMGPATRHGHCAASIADCRDILQMQQLLSALIKRLT
                     RETVVQLTDFR"
     gene            complement(26284..27369)
                     /gene="ypdF"
                     /locus_tag="OO130_000982"
     CDS             complement(26284..27369)
                     /gene="ypdF"
                     /locus_tag="OO130_000982"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCR5364480.1"
                     /translation="MTLLASLRDWLKEQQLDAVLLSSRQNKQPHLGISTGSGYVVISR
                     ESAHILVDSSYFVEVEARAQGYQLHLLDATNTLTTIVNQIIADEQLQTLGFEGQQVSW
                     ETAHRWKSELNAKLVSATPDVLRQIKTPEEVEIIRLACGIADRGAEHIRRFIQAGMSE
                     REIAAELEWFMRQQGAEKASFDTIVASGWRGVLPHGKASDKIVAAGEFVTLDFGALYQ
                     GYCSDMTRTLLVNGEGVSAESHPLFDVYQIVLQAQLAAISAIRPGVRCQQIDDAARRV
                     ITEAGFGDYFGHNTGHAIGIEVHEDPRFSPRDTTTLQPGMLLTVEPGIYLPGQGGVRI
                     EDVVLVTPQGAEVLYAMPKTVLLTGEA"
     gene            complement(27384..28631)
                     /locus_tag="OO130_000983"
     CDS             complement(27384..28631)
                     /locus_tag="OO130_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HCR5364481.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGVIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(28653..28979)
                     /locus_tag="OO130_000984"
     CDS             complement(28653..28979)
                     /locus_tag="OO130_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-like phosphotransferase enzyme IIB
                     component 1"
                     /protein_id="HCR5364482.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(29198..30163)
                     /gene="glk"
                     /locus_tag="OO130_000985"
     CDS             complement(29198..30163)
                     /gene="glk"
                     /locus_tag="OO130_000985"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HCR5364483.1"
                     /translation="MIKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            30367..31623
                     /locus_tag="OO130_000986"
     CDS             30367..31623
                     /locus_tag="OO130_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HCR5364484.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTINIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            31738..32064
                     /locus_tag="OO130_000987"
     CDS             31738..32064
                     /locus_tag="OO130_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCR5364485.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERSYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(32205..33443)
                     /locus_tag="OO130_000988"
     CDS             complement(32205..33443)
                     /locus_tag="OO130_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HCR5364486.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKTLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVLPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            33779..34981
                     /gene="nupC"
                     /locus_tag="OO130_000989"
     CDS             33779..34981
                     /gene="nupC"
                     /locus_tag="OO130_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HCR5364487.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
BASE COUNT         9249 a         8728 c         8109 g         9036 t
ORIGIN      
        1 gtcacggcga actgcctttc aaggttgaag tggcacgacc tcattggctg aaggtttacc
       61 ttatgttggc ctgaccgaac aggatgttca ggacgcccat gcgcgcttat cccgttttgc
      121 accctatctg gcaaaagcat ttgctgaaac tgctgccact ggggggatta ttgaatcaga
      181 actggttgcc attccagcta tgcaaaaacg gctggaaaaa gaatatcagc aaccgatcag
      241 cgggcaactg ttactgaaaa aagatagcca tttgcccatt tccagctcca taaaagcacg
      301 cggcgggatt tatgaagtcc tggcacacgc agaaaaactg gctctggaag cggggttgct
      361 gacgcttgat gatgactaca gcaaactgct ttctccggag tttaaacagt tctttagcca
      421 atacagcatt gctgtgggct caaccggaaa tctggggtta tcaatcggca ttatgagcgc
      481 ccgcattggc tttaaggtga cagttcatat gtctgctgat gcccgggcat ggaaaaaagc
      541 gaaactgcgc agccatggcg ttacggtcgt ggaatatgag caagattatg gtgttgccgt
      601 cgaggaagga cgtaaagcag cgcagtctga cccgaactgt ttctttattg atgacgaaaa
      661 ttcccgcacg ttgttccttg ggtattccgt cgctggccag cgtcttaaag cgcaatttgc
      721 ccagcaaggc cgtatcgtcg atgctgataa ccctctgttt gtctatctgc cgtgtggtgt
      781 tggcggtggt cctggtggcg tcgcattcgg gcttaaactg gcgtttggcg atcatgttca
      841 ctgctttttt gccgaaccaa cgcactcccc ttgtatgttg ttaggcgtcc atacaggatt
      901 acacgatcag atttctgttc aggatattgg tatcgacaac cttaccgcag cggatggcct
      961 tgcagttggt cgcgcatcag gctttgtcgg gcgggcaatg gtgcgtctgc tggatggctt
     1021 ctataccctt agcgatcaaa ccatgtatga catgcttggc tggctggcgc aggaagaagg
     1081 tattcgtctt gaaccttcgg cactggcggg tatggccgga cctcagcgcg tgtgtgcatc
     1141 agtaagttac caacagatgc acggtttcag cgcagaacaa ctgcgtaata ccactcatct
     1201 ggtgtgggcg acgggaggtg gaatggtgcc ggaagaagag atgaatcaat atctggcaaa
     1261 aggccgttaa taacgtttca acgcagcatc gcaatccttt ccctgggtga gcgatgctgc
     1321 cgatggcgca gacttaagat ccccggtctt acccgctata accccctcct tttatcatca
     1381 cccaacgcct ttcgctgtaa acggcggttt cgcaaaccaa accaaaaccg taagtaagat
     1441 aaaagcaata gccgccatac ggaaaatctc atttgcagaa atagaaagtg actgctgggt
     1501 tatttcatta ttaatttcat taagaactcc tgaaagcgaa ccataatatt tatccataat
     1561 ttgtgacgat gaattaaaca cggggttaaa ttgatcgatg gttgctgtca actgactatg
     1621 gtgtaacgat tcgcgtcgtc cccacagcgt cattgtcaac gacgtaccaa ctgatcctga
     1681 caaggtacga aaaaaattac tcatactcga ggcattggca aatttattat ctggcaagcc
     1741 tgaaaacgaa atcgttgtta agggtaaaaa gaaacaggca acggcgaatc cctgaaaaaa
     1801 ctgcggcaaa atgatgcctg taaaatcaat cgttggcata aatgtcacag aacgccagta
     1861 atagcaaacc gcatacatca aaaaactaaa tgtcactaac aaccgcatgt ctattttgtt
     1921 gccataacgt cctatcaaag gtgaaattaa tagtggcatg atgccgatgg gcgcataagc
     1981 aagtccggcc catatcgcat tataccccat cgtttcctgg agtaactgcg gcataaggac
     2041 gatcgctcca gagtaaaata aatacgcgca tgtgatactc acaataccaa tggtgaagtt
     2101 acgggactta aacaaactga gatcaagaat cgggttctct gaggtcgact cccaaatgac
     2161 taaagagatc agagaaataa ctgatactac tgttaatatt attattgtac tcgagttgaa
     2221 ccaatccaga tcgcgccctt tatcaagcat aatttgcaag ccaccaacac cgagcactaa
     2281 cagggtcagt cctggtagat tcattttgac cggtgaagtc tcagtttctc ttcctttaag
     2341 taaggttaag cataatgtca ggacgataat ccccataggg acattgatta aaaatatcca
     2401 accccagcta aagttatcac aaatataacc gcccaatatc ggcccacata tcggagcgat
     2461 aatcacggtc attgaccata atgccagagc aaatgttctt ttttctggcg gataattcct
     2521 taataataaa ctctgtgaca gtggaattaa cggccccgcc attaacccct gaacgactct
     2581 aaaaaatatc agcacatcaa gattggtcga taggctacac attaatgaag acagcgaaaa
     2641 aaaggttact gaaagtaaaa ataatcttaa ttcgcctatt ctttgtgcca acctgccagt
     2701 aacagggatc gcaatggcat ttgctacacc aaacgaggtg ataacccagg tgccttcgtc
     2761 tgttgatgct cccagaaagc cagatattgt cggtattgcg acgttagaaa tagtggaatc
     2821 caacatttgc ataaatgtcg ctaatgacaa tgcaatagtg acgcaccata acgtcccacc
     2881 ggttaatggt gccggagtcg atttagtgat tgccattaaa gttgtccatt atgcgaaata
     2941 atgttgctaa tttctttttc tatcggactg gtatcgataa ctaaagcctt actggtataa
     3001 gccggcatgg aggtcacggt tgaagccagc tcaggcatct cggcaatgtc ttcgttcttc
     3061 gtatcaatag ttgctgtcat cgataaacca atacgcaagg gatgttccat gagttctttt
     3121 ggatcaagag aaacttcaac cggtacacgc tgaacgattt tgatccagtt ccctgtcgca
     3181 ttttgtgcag gtaataagga gaacgcattg ccggttccca tattgatccc tgtcacccga
     3241 ccatgaaaca caacattttc accataaaga tcgctgataa tattgaccga ttgaccaatc
     3301 cgcacatccg tgagttgtgt ttctttaaag ttggcattaa cccacatttg acgtgccggt
     3361 acgacagcca ttaacgattg tccggggctc actgtttcgc cgacctgaac acttctctgg
     3421 gcaatatagc cggtaaccgg actcttaata tccgtacgtt taagcgccaa ccaggcttct
     3481 ttagttgcat ccgccgcttc aacgacttgt ggctgacggt ttaatggtgt gttcattact
     3541 aaagctttat tcgctttata agcctggata gcggcattca atgccgcttt gctacttatt
     3601 aacgtatctt tggtatgctc cagcgtttct tttgaaataa ccccctgctt cgctaacggc
     3661 actcgacggt tataatcttc taaagattgt tgatactgaa tacgtgctga agcgacttcg
     3721 gcactgtatt gtttatcctg taagtatagt ttattcgttt gccgaacaat atttgccaga
     3781 ttatttttag ctttattgag tgcgatagtg gcatcagttt tatccagtga aactaaaatg
     3841 tcaccttgtc gaacgtagtt cgtatcttta tgattaacga cagtgacact acctgagact
     3901 tgtgcagaaa ttggatctgc attccccgtg acataggcgt catctgtact aatcatgtct
     3961 tctaattcca ttgaccaata gacataggca cctgaaaacg caataaataa aactaccgcc
     4021 aataaagaaa agtattttct tctgttagaa tgttttttat ttgaattaat ctgttccact
     4081 attatctctc atttctcata gatgaaattt atgaattgaa tgaaaagagc attcattctt
     4141 catatcaaac acagtgcatg tgttgcacat tatcccagga acgttggagg cctaatttaa
     4201 atgaggtgca aaaaaaacaa cagaggacta aaccgtggct tttgcaatac aattcttacg
     4261 cctgtaggat tagtaagaag acttatagtg ctaacttgaa actataaatc atcggtacaa
     4321 cccctgattt tattgttgac atttcattta tgccgactat ttatatggta tacttgtcga
     4381 attatcttaa aggaagctca gattttctta tttttattga gaaaatgaga tgacgcctta
     4441 tgtctgtatt actacaggga gaagggagat gcttcattgc aaagggaata atctatgaac
     4501 gcaataatta ttgatgacca tcctcttgct atcgcagcaa ttcgtaattt attgatcaaa
     4561 aacgatattg aaatcttagc ggagttgact gaaggggcaa gcgccgttca gcgggtggaa
     4621 acacttaagc ctgatatcgt catcattgat gtcgatatcc ccggagttaa cggtatccag
     4681 gtgttagaaa cgctgaggaa gcgccaatat agcggaatta ttattattgt ctccgctaaa
     4741 aatgaccatt tttacgggaa acattgtgct gatgctggcg ctaatggatt cgtgagtaaa
     4801 aaagaaggca tgaacaatat cattgcggct attgaagctg caaaaaatgg ctactgctat
     4861 ttccccttct ctctcaaccg gtttgttgga agtttaacgt ccgaccagca aaaactcgac
     4921 tccttatcga aacaagaaat tagtgtcatg cggtatattc ttgatggcaa ggataataat
     4981 gacattgctg aaaaaatgtt catcagcaac aaaactgtca gcacttataa aagtcgcctg
     5041 atggaaaaat tagaatgtaa atcactgatg gatctttaca cattcgcgca acgtaacaaa
     5101 atcggctaat cacatgaagt ttttacccta tttttttctt ctctgttgtg gtctttggtc
     5161 gaccataagt ttcgcagacg aagattacat cgaatatcgt ggcatcagta gtaacaaccg
     5221 tgtcacactt gatccagtac gtctgagcaa caaggaatta cgttggttag cgagcaaaaa
     5281 aaatcttgtg attgcagtac acaagtccca aacggctacc ttgttgcata ccgattcgca
     5341 gcaacgggtt cgtggtatta atgctgatta tttaaatctt ttaaaaagag cgttaaatat
     5401 caaattaaca ctccgggaat acgccgatca tcaaaaagca atggacgcgc tggaagaagg
     5461 tgaagtcgat atagtgttat cacatttagt tgcttcgccg cctcttaatg atgacattgc
     5521 tgcaaccaaa ccactgataa ttacctttcc ggcgctcgtc accacccttc acgattcaat
     5581 gcgaccgctt acctcatcaa aaccagtaaa tattgctcga gtagcaaatt atccccctga
     5641 cgaggtaatt catcaatcat ttccaaaagc aacaattatc tcttttacaa atttatatca
     5701 ggcattagca tccgtctcag ccggacagaa tgattacttt attggtagta acatcattac
     5761 cagcagtatg atttcccgct atttcactca ctccttaaat gtagtgaaat attataactc
     5821 accgcgtcaa tataattttt tcttgaccag aaaagaatct gtcgttctta atgaagtact
     5881 caatcgattt gttgatgctt taacaaatga agttcgctat gaagtttcac aaaattggct
     5941 tgatacagga aacctggcct ttctgaacaa accattagaa ctcactgaac atgaaaaaca
     6001 gtggattaag caacatcccg atttaaaggt gctggaaaat ccttactctc ctccctattc
     6061 tatgacggat gaaaatggct cggttcgggg cgttatgggg gatattctta atattattac
     6121 cttgcaaaca ggtttaaatt tttctccgat caccgtttca cacaatatcc atgccggaac
     6181 acagcttaac cccggtggat gggatataat acctggcgct atttatagtg aagatcgaga
     6241 aaataatgtt tcatttgcag aagctttcat aacaacgcca tacgtttttg tcatgcaaaa
     6301 agcacctgac agtgaacaaa cattaaaaaa aggaatgaaa gttgccattc catattatta
     6361 tgagctgcat tcgcaattaa aagagatgta tccggaggtt gagtggataa aagtcgataa
     6421 cgccagcgct gcatttcaca aggtcaagga aggtgaactt gatgctctgg tcgcgacaca
     6481 gctaaattcg cgttacatga tcgatcatta ctatcctaat gaactgtatc attttcttat
     6541 tcccggcgtt ccgaatgcat cgctttcgtt cgcttttcct cgcggagaac cggaacttaa
     6601 ggatattatt aataaagcac tgaatgcaat tcccccaagc gaagttctgc gcctgacgga
     6661 aaaatggatt aaaatgccca atgtgaccat tgacacatgg gacctctata gcgagcaatt
     6721 ttatattgtt acgacattat ccgttttatt agttggcagt agccttttat ggggattcta
     6781 cctgttacgc tcagttcgtc gtcgtaaagt cattcagggt gatttagaaa accaaatatc
     6841 attccgaaaa gcactctcgg attccttacc aaatccaact tatgttgtaa actggcaagg
     6901 taatgtcatt agtcataata gtgcttttga acattatttc actgcggatt actacaaaaa
     6961 tgcaatgtta ccattagaaa acagtgactc accctttaaa gatgtttttt ctaatgcgca
     7021 tgaagtcaca gcagaaacga aagaaaatcg aacaatatac acacaggtat ttgaaattga
     7081 taatggcatc gagaaaagat gcattaatca ctggcataca ttatgcaatc tgccagccag
     7141 cgatcatgct gtttatattt gtggttggca agatattacc gagacgcgtg atttaattca
     7201 tgcactcgaa gtagagagaa ataaagcgat caatgcaact gtcgcaaaaa gtcagtttct
     7261 ggcaacaatg agtcacgaaa taagaacacc aataagctcc attatgggct tcctggaact
     7321 tctgtcgggt tctggtctta gcaaggagca acgggtggag gcgatttcac ttgcctacgc
     7381 caccggacaa tcactcctcg gcttaattgg tgaaatcctt gatgtcgaca aaattgaatc
     7441 gggtaactat caacttcaac cacaatgggt cgatatccct actttagtcc agaacacttg
     7501 tcactctttc ggtgcgattg ctgcaagcaa atcgatcgca ttaagttgca gcagtacatt
     7561 tcctgatcat tacctggtta agatcgaccc tcaggcgttt aagcaggtct tatcaaattt
     7621 actgagcaat gctctcaaat ttaccaccga gggggcagta aaaattacga cctccctggt
     7681 tcacattgat gacaaccacg ctgttatcaa aatgacgatt atggattctg gaagtggatt
     7741 atcgcaggaa gaacaacaac aactctttaa acgctacagc caaacaagtg caggtcgcca
     7801 gcaaacagga tctggcttag gcttaatgat ctgcaaagaa ttaattaaaa atatgcaggg
     7861 cgatttgtca ttagaaagtc atccaggcat aggaacaaca tttacgatca caatcccggt
     7921 agaaattagc cagcaagtgg cgactgtcga ggcaaaagca gaacacccca tcacactacc
     7981 tgaaaagttg agcatattaa tcgcggatga tcatccgacc aacaggctat tactcaaacg
     8041 ccagctaaat ctattaggat atgatgttga tgaagccact gatggtgtgc aagcgctaca
     8101 caaagtcagt atgcaacatt atgatctgct tattactgac gttaatatgc cgaatatgga
     8161 tggttttgag ttgactcgca aactccgtga gcaaaattct tccttaccca tctgggggct
     8221 tacagccaac gcacaggcta acgaacgtga aaaagggtta agttgcggca tgaacttatg
     8281 tttgttcaaa ccgttgaccc tggatgtact gaaaacacat ttaagtcagt tacaccaggt
     8341 tgcgcatatt gcacctcagt atcgccacct tgatatcgaa gccctgaaaa ataatacggc
     8401 gaacgatcta caactgatgc aggagattct catcactttc cagcatgaaa cgcataaaga
     8461 tctacccgct gcgtttcaag cactagaagc tggcgataac agaactttcc atcagtgtat
     8521 tcatcgcatc cacggtgcgg ctaacatcct gaatttgcaa aagttgatta atattagcca
     8581 tcagttagaa ataacacctg tttcagatga cagtaagcct gaaattcttc agttgctgaa
     8641 ctctgtaaaa gaacacattg cagagctgga ccaggagatt gctgttttct gtcagaaaaa
     8701 tgactaaata gcggctccca caatgttcaa atgtgggagc tatttaccag cacatcttac
     8761 atttatgatg agaactcctg gcgaatttgt tccccatgct ggtcgagcgt tgccgctccc
     8821 ggcataacat gcgggtccgc gcagccgctg atttttatcg gattacccgg catcattatt
     8881 cccccggctt caatcaacat atttctcgcc tgagtttgtg gcaatttaat ggcctcagcc
     8941 acacttaata acggcgcgac gggtacacca acttcatgta ttctggctaa ccaaacttca
     9001 gctgcctgcg tttttaacgt ccgctcaata tattgtttaa gaatagcctg gttttgtacg
     9061 cgtaaaatat tgctgctaaa tcggggatca ttaaccagtt ccgtaagctc cagtgcctgg
     9121 cataacgcag aaaaaagctt gtcattacca caacaaatcg taatcggctt atcctgagta
     9181 ttgaaaacat caaaaggtgc catgtaggga tggcgatttc ccagacgttg tggtgacttc
     9241 cctgtcgcga tatatgccat cagaccatgc tccagaaaac tcagcgtggc atcaaacatc
     9301 gctatatcga catgcgcccc tctctggctc ttttcgcggc cataaagtgc actcactatt
     9361 ccgctgaata aatagacacc gccgcataga tccgcaagag atgtaccaac gcgcactggc
     9421 ggagcatcag gatatcctgt ttccatcatt atcccgctca ttgcctgaat gatggtatcg
     9481 taggcaggag catcttttag cggaccggta tgtccgaaac ctgacgatga agcatatatg
     9541 aggcgcgggt tgatttcttg aagcgtttcc catgaaaacc ccagtttttc cattgtacct
     9601 gggcgaaaat tctcagctaa tacatcagct tgtttgagca tatttataaa tatactttta
     9661 tcgtaatcat tctttaaatc aagaaccaca ctctctttgc catgattaat aaaactgtaa
     9721 tagagtgact gtccatccac atagggacca aatgtgcggg tatcatcacc atgacccggc
     9781 ggctcaactt taattaccct tgcgcccata ttacaaagaa gttgagttcc gaaaggtcca
     9841 ttaaggacat gtgtcatatc gataactaat aagccttcaa acggcccttt gctttcatta
     9901 tttgtcattt gcttatgcct tgaaaaaagc cgaaggtgaa aagacacttc ggcatttatg
     9961 taaaatcaat aacatgatta attatgaaac cagtcgactg acataaatcc acaagggagc
    10021 cgtgacgaca aagcccagta cgctcacagc caatgacgct gtaccagtac gggtgtagac
    10081 attaaaccgg ctggcaataa taattccgga gaatgccggc ggtaatgcgc ctgccagtac
    10141 catcatctgc agatgttcgc tgttcaaatg acatgccata ccaacgagaa gcagtgccag
    10201 tggcatcaga atcagcttca ggaaggtgtt ataagcaatt tcggcactga actcgaattt
    10261 atgtgcagcc agagtcaacc ccgcagcgaa taccgctacc cctgagttag ccttcgcaat
    10321 cagattaaag gttgggtccc atgctgccgg aatttttacc ccaaccaaca ccaggatcgt
    10381 tgccagaaca ggtgcccata ctactggctc ctttgccgca gaaattaatg cactcagatt
    10441 actattcttc ttaccatccg ctcctgaaga aggattcagc aaatacagac caataggaat
    10501 agtaattgcg ttaacaataa tagaaataat tgctaccact aaacctgttg atacggaatc
    10561 accataaata ggatcgagaa ctgcaaaccc caggaatcca atggtaggtg aacctgcaat
    10621 taatgcacat acagctgctt ctgcatgggt acgtttaaaa aatttgtagc aaccgaacca
    10681 ggagaaaaag aaacatccga caataaccac aagtgataca agggtcagac gagtgtccgc
    10741 aaaaatcatt tccctgtttg cccgagtaat agatacaaat agagccgcag gaagcgcgta
    10801 gtttaatacc agtttattaa atgcgcgggc ttgatcttct gaaaaagttt ctcgtctgcc
    10861 gctaaaataa cccaacagca tgattacgat aataggcaat aaatcgccaa taaaaaatgt
    10921 tagcataata gtatttccga taagggtccc ctcaccatcg cataatgagt tggcaagagg
    10981 agacgatgtc agtatttaat taccagcgac ttgttttggg ttaagtttgg taatatggcc
    11041 actttcagtt cctgctgcag ggtcgatgac cacattaata atggtcggtt tgcgcgactg
    11101 aataccggtg gttaaagcat gacgaagttc atctgtcgtg gtgacgttat agccaacgcc
    11161 acgaaacgca tccattaatt tgtcatacct tgcatggtgc aacagatccg ttggagatgg
    11221 tgcaccagcg ccactgagat caacaccgtc tcctctgtag atgccgccat tattaaaaat
    11281 aacgatcgtc accggcaggt tatatcgaca aatcgtttca atttccatcc cactgaaacc
    11341 aaaagcacta tcaccttcaa tggcgacaac cggagaacca gaagtcacgc tagcaccgat
    11401 ggcatagccc ataccgatgc ccatgacacc ccaggtacca caatccagac gacgacgtgg
    11461 tttatacata tcaataatat ttcgtgcatt atccagggta tttgcacctt cattaactaa
    11521 ataaatatcc tggttctcgc gcaatacgtc gcgcacagaa cttaatgcat taaagtaatt
    11581 taatggttga gtatctgtac ttaatttttc atgcattttt tgtgcatttt gctgcttgtg
    11641 gatatttaaa atatcgcgcc ataccagtgg agtcgtaaat gtgttttgtt tcagttctgc
    11701 cagcatacct tgcatactgg atgcaatatc accaatgact ggcacagcaa tggggcggtt
    11761 gctgtcaatt tcctgcggtt caatatccag ttgaataaac tgtgtatctg ccgcccatcc
    11821 ttttttaccg tgtgccaata accaattcag tcgtgcacca acaagcatga caacgtcagc
    11881 atttgccagg gcaaacgaac gcgcagctgc cgcagaaagt ggatgcgtat cttcaaggat
    11941 ccctttcgcc atagacattg gcaggaatgg aatctgagca ctttcaataa attcacgaag
    12001 ctgttcatca gcttgtgaat acgccgcgcc tttgccaagg ataattaatg gccgttcagc
    12061 ttttgctaaa agcgaaattg cgctagtgac tgacttcggg catggcaata atgctggcga
    12121 cggattttca actttaacaa tcgtggttaa cgcttcgtct ttttccatcg tcgcggccag
    12181 gacatttgct ggcaaatcaa gataaactcc gccagggcga cccgatacag agacccggat
    12241 agcgcgtgcc aatgcaatgc caagatcctg cggctgatta acgcgaaatg ctgctttggc
    12301 atacggtttt gccgcattca tttggtccag ctcttcataa tcaccttgct gtaggtcgac
    12361 gatcgcgcgg tcgctggagc cgctaatcat gatcatcgga aaaccattta ccgttgcgtt
    12421 ggccaatgcc gtcaaaccat tgaggaatcc tggcgcagaa actgtcaggc agatccccgg
    12481 tttttgggta agaaaaccgc ttgccgcagc ggcatagcct gccgactgct catgacgaaa
    12541 accaatataa cgaatgcctt ccgcctgggc atggcgtgcc atatccgtca caggaatacc
    12601 tacaacacca taaatagtgt caatattatt ctgttttaat gcttcaacga tgatatgcat
    12661 accatctgtc atttgaagtt gatctgacat gattaataac ctcgaaattt ttataaattg
    12721 ggagccagaa gcccccgtta atatcagatg gcgtggtttt gcttcattgc agcaatttca
    12781 tcgtcgctat aacctagctc ctgcaataca gcagcggtat gttcacctaa tagcggcgca
    12841 gctttaatat ccggcgtaaa agcagagaat ttcattggac aaccaacggt cagatatttt
    12901 ccacgcaacg gttgttccac ttcaacaaca ctgccacttt ggcgcaaaga gggatcaagt
    12961 gaaatttctt tcatacttaa aaccggtgca caaggaatat caaactgagt caaataggcc
    13021 accgcttcat gtttatcaat agtgacagtg tatttttcga tttcagcaaa aatatcgaaa
    13081 atatgtggct gtcgggcatg ggctgtactg tatgccggat cggtaatcca ttctggtttg
    13141 ccgatggctt tacaggtgtt ttcccagttt tgctcctgaa tagtgaaata aatataggcg
    13201 ttcggatcgg tttcccagcc tttacatttc aggatccagc caggctgacc gccaccacct
    13261 gcattaccac cgcggggaac tgcatcacca aatgtaccat tcggatactg cgggtattct
    13321 tccagataac ccaatttatc gagacgctgc tggtcacgta atttcacgcg gcaaaggttc
    13381 aatacggcat cctgcattga catggtgact cgttgcccac gccccgtttt ttcgcgatgc
    13441 agcaaagcag caagcaaacc gatcagcaaa tgcattccgg tgttgctgtc acccaacgct
    13501 gcagcgctta ccagcggcgg accatcccaa aaacccgtag tggatgccgc gccacccgct
    13561 gcctgagcaa cgttttcata ggcttttaca ttcacataag gcgaacactc atcaaaccct
    13621 ttgatcgaac caaaaatcag acgtggattg atttcttgaa tatgctccca ggtgaagccc
    13681 atgtgatcaa tggcccctgg atgaaagttc tcgactaaga tatcagcttc gcggatcagc
    13741 ttttccatta cctctttgcc ttccgctgtt ttggtattta actcaataga acgtttgtta
    13801 ctgttaagca tggtgaagta aagcgcatcg atatcaggaa tatctcgcag ctggtgacgc
    13861 gttacgtcac caacgccggg acgttcaatt ttaataacgt cagcgccaaa ccaggccagc
    13921 atttgagtac aagatgggcc agattgcaca ccggtgaaat cgagaacttt aattccttga
    13981 agtggagttg acatagcaat acctattagt taattaaata tgtgttaatt tagcgtttat
    14041 ttaaggcgta atatcttcta taaataaccc gcaaaatatc gtcgtagctg cactcgatat
    14101 ttttaaaatc ctgtatcaag tgctgaaaat catttcatcg ccacttccaa ttcctttctg
    14161 catggtaagt agtgaggacg aaaagaagaa tatgataaat attgattcgt ggtaatcaga
    14221 gagagtcgcg acatttgtga tgttattctt acaactgtaa gaagcgccgg tgatttacaa
    14281 gtaagggtat gattacgctt tttaaagcgt accataaaac ataacataat aaatgatttg
    14341 tgatgacacg ggattattgg ggtttacttt gaggaagata aaatgcgtgt ttatagaacc
    14401 gcgagcgcca aataatttta tgaccacaac ctgtcgtatt gtggtcataa aaaattgtcg
    14461 gctggaggga gttaaatcct ttacctcaat atactgcctg tcagcattag ttcacaaaca
    14521 gtgcttcgct gtcgacgatg attccatctt ccgcaccttt cagcgccagg tttgcctcgt
    14581 agtattgctt tttatcaagc agtttctgcg catcagcaag tgcattacga gtctgtttaa
    14641 gcggcatcag atattggttt tccattacgc caacaccggc caggcgaagt tcttccattg
    14701 cgcccttttt atcccctttc gccatttttt cattggcaat ttttatcgcg gcttcttttt
    14761 cgggagttgc tacataactc tcagaaattc cgacagatgc attaatgaca atatattggt
    14821 catcattgag attggtcttt ttacctggct tagcgaactt tgcccattcg gtactgtcat
    14881 ctgacagcaa agcggaagct tcattggtca gttcttttgc cttttctgga tcgccatgaa
    14941 acagagccag gcgcgctacc tgcacgtcac gcatcgcata taaaccttgt tcagagatac
    15001 gctctgctgc tgccgttttc tgggtttgct gaacttgctg agtttgctgt tgtgccgcta
    15061 ccggtgcatt atcagcagcc catacagttg aagatgccag aattgctgtg accatcgtag
    15121 ccataattaa acgtttcata cttacctcat tctttaacag ttttagaaaa cgccgccatc
    15181 atattgggtg agcatgaaat gagaaatcag agagcaacgg tcattttgaa agggttatct
    15241 tacagttgta gttattatcg caagaagtat tttagtctta caattgtagg aatctgcttc
    15301 ctgtcgcaga gaactattct tatgtaccac aaagagatgt catttattat atcgatgtaa
    15361 atattgatta attatatgta aatacagcca taacatcgta ggttctcagt gaaccatttt
    15421 caggataact ttctatgaaa gtaaacttaa tacttttcag cttattttta ttggtctata
    15481 ttatggcatg caatgttttt gcattttcca tttctggtgg tgtaagtgag aggagctata
    15541 aagagactga aaaaacatca gcgatgacga ccacacactc tacaaaactt cagccatcac
    15601 aggcgatttt gtttaagatg agagaagatg cgccaccatt aaacctcaca gaagaaatca
    15661 cgcctactta tccgacaaag gcgaattatc ttattcatcc tgtgcgatag cgtctgtcac
    15721 atggcaggtg aacaatcatg tctgatgctg atgtgaacac ctgcccaaat gagccatcct
    15781 gacccactat cgcctgccat accccaccat aaagcgaatc atcaagccac aagccggggc
    15841 aagaaacata aatatctccg tcactagttc attaccgcca gttaaccaca tggtgaagta
    15901 ataaatgagt gcagcgagga tcaccatgcc gaagaaagaa gagaccgccg ataccacgtt
    15961 cagtacctga ccgatgtagc cgcttacgca gacaatacac agggcgagaa acatccataa
    16021 ataaatcata gctcaacctc ctgccgcgct aaaaaacctg taacttcagt cactttaata
    16081 gtaagacaat ccttaacctc tggcaatttt tgctaagcat caatgaaaac agcatgttaa
    16141 atgcaagact gttgtgtacg gaaaaatatt tactttgcac gattaataat cagtagctga
    16201 aagcagtcag cgagtgataa agtacagata ccgcattcat gctcctcgtg gttatgtcct
    16261 gactagtctt tacacacttt acaggaacca ttgtcgtaca tgatggccca accaattgag
    16321 tgtaccgctg tcgacatgga atcttctgcg atacaacaat tcgtatctac agaaggtaac
    16381 tatgtttcca caatgcaaat tttcccgcga gtttctacat cctcgctact ggctcacatg
    16441 gtttgggctt ggtgtactct ggctttgggt acagcttcct tatcctgttc tctgctttct
    16501 cggcacgcgt attggcgcaa tggcgcgacc attcctgaaa cgtcgtgaat ctatcgcccg
    16561 taaaaacctg gaactttgtt tcccgcagca ttctgcggaa gaacgcgaga agatgattgc
    16621 cgaaaacttt cgttcactcg gcatggcgct ggtagaaacc ggcatggcat ggttctggcc
    16681 cgacagtcgc gtacgtaaat ggtttgatgt tgaagggttg gataacctta aacgcgcaca
    16741 aatgcaaaat cgcggcgtaa tggttgtcgg cgtccatttt atgtcgctgg aactgggcgg
    16801 ccgcgtgatg ggactgtgcc aaccaatgat ggctacctat cgtccacata ataatcagct
    16861 gatggaatgg gtgcagaccc gtgggcgcat gcgctctaac aaagcgatga tcggcagaaa
    16921 taatctgcgc ggcattgtcg gtgcactgaa gaaaggtgaa gcggtatggt ttgctcccga
    16981 tcaggattat ggtcgtaaag gcagctcctt cgcgccgttc tttgcggtgg aaaatgtcgc
    17041 cacaaccaat ggcacctatg ttctctcccg tctctctggc gcagccatgt tgaccgtaac
    17101 gatggtaaga aaagcggatt acagcggata tcgtttgttc atcaccccag agatggaagg
    17161 ctacccgaca gatgaaaatc aagccgctgc ctatatgaac aagattatcg agaaagagat
    17221 catgcgcgca ccggagcagt acctctggat ccactgtcgc tttaaaacgc gcccggtggg
    17281 agaatcgtcg ttgtacattt aacaaactgc tcttactctt tccctcctca aatcggccat
    17341 aataacctca tggtttttat ggcttttttc ttaaggtttt ttccttcgct gaatatctaa
    17401 ttattaccgt cagaaattaa actgtcgccg atatgagaca cttgtaagtt actaaaagta
    17461 aataagaaaa tgcctcttgt ctcgtttcag ttttaggcgt acattagcgc cgtctcgagc
    17521 agtttagctc agaattctgg ggtgatgccc acggtaaaat acagggcttt taaggtcgct
    17581 ttgcccagtg tcaactcact gtatcaggtg taatgaagtc attcaggcgt aacagcaatt
    17641 acgcggagag atgtaaagtg aaatatttct ttatgggcat ttcttttatg gtcatcgttt
    17701 gggccggtac ttttgccctg atgatctaaa agcaaactgt gcaatgaaaa acaggagcca
    17761 tcaggctcct gtttgcattt ctgacgacgg tgtttattcc gcgttttcgt gaatatgttt
    17821 gctgctggcg ggtagtaaac cgtcggcacg gaacatcgct ttaatccctc tgatggcctg
    17881 acgaatacgg tcgcggtttt caatcagtgc aaagcgaaca tgcgtatcac catagtcgcc
    17941 aaagccaatc cccggcgaga cacagacctt cgcttcgtta agcaatttct tggcaaatac
    18001 cagcgagccc atggcagcat acggttccgg tattttcgcc cagacataca tcgaagcctt
    18061 cggcatttca actatccagc ccgcttcatg cagcccttta accaatacat cacggcggcg
    18121 tttgtactgt tcggcaatgt cgcgcacgca ctgttgatcg ccctccagcg ccgcaatcgc
    18181 cgccacctgc aacggcgtaa aggtgccgta atcgtgatag cttttaatac gagccagggc
    18241 gctgaccagc gttttgttgc caaccataaa gccgatacgc cagcccgcca tgttgtagct
    18301 tttcgacagc gtaaagaact cgactgccac atcgcgtgca cccggtacct gcatgattga
    18361 cggcgctttc cagccatcgt agacgatatc ggcataggcc aggtcgtgga ccaccagcac
    18421 atcgtagcgt ttcgccagcg ccacaacctt ttcgaagaac tccagctcca cgcattgcgc
    18481 ggttggattc gacgggaagc cgaggatcat catcttcggt ttcggataac tttcacgaat
    18541 ggcgcgctcc agttcgttga agaaatcgac accttccacc agcggcactg agcgtacctg
    18601 cgccccggca atcaccgcgc cataaatatg gatcgggtaa cttggattcg gcaccagcac
    18661 cgtgtcacca tgatccagcg tcgccagcat cagatgcgcc aggccctctt tcgaaccaat
    18721 agtgacgatg gcttctgatt ccgggtcgat ttcaacgtcg tagcgatcct gataccagcg
    18781 ggaaatggcg cgacgtaacc gcggaatgcc gcgtgaagtg gagtaaccat gcgtgtcctg
    18841 gcgctgggcc acagtacata atttttcgac gatatgcggc ggagtcgcac cgtccgggtt
    18901 acccatgctg aaatcgataa tatcttcgcc gcgccgacgc gcagccattt tcagttcagc
    18961 ggtgatgtta aaaacgtagg gcgggagacg atcaatgcgc gtaaagcgac gttcagggcg
    19021 agtgtcagcc ataatttcct cagattaacg ttagcgcccg gaccgtccga gcgacgctgc
    19081 cacaatggtg gctgttttga aaatagcctg attaatttct gtctgtccag cgttttagaa
    19141 catttttatc aatggcttta aatgaggaac tccacttcaa aaacaggaaa tttttctacg
    19201 ctaacaagaa aattaccacc aattttatta acattataat ttaaataaat ttaccagcca
    19261 gaagtgcatt atcaagagat acattgctaa tacaccattt atctttcaat ccccttttaa
    19321 atttatggtt tttcctcaaa aacggcctgc tggtcatcag ccttcaggtt acctatcata
    19381 gaggtttaat ccttattcag agtcacccgt gcacgaaata ttcaacatgc tgctggcggt
    19441 cttcgatcgg gcagcgttaa tgcttatctg cctgttcttt ctcatccgta tccgcctgtt
    19501 tcgcgagctg ttgcacaagt cggcacactc cccaaaagag ttgcttgctg ttaccgccat
    19561 tttctcgctg ttcgccctgt tcagcacctg gtccggcgtt cccgtagaag gctcgctggt
    19621 gaacgtacgt attatcgcgg tgatgtccgg cgggattctg tttggcccgt gggtaggcat
    19681 cattaccggc gtgattgcgg gtattcaccg gtatttaatt gatatcggcg gcgtgacggc
    19741 gatcccctgc tttatcacca gcattctggc gggttgtata tcaggctgga taaacctgaa
    19801 aatccccaaa gcacagcgct ggcgcgtcgg tattctcggc ggcatgttgt gtgagacgtt
    19861 gaccatgatt ctggtaattg tctgggcacc aactaccgcg ttggggatcg atatcgtctc
    19921 taaaatcggc attccaatga tcctcggtag cgtctgtatc ggctttattg tgcttctggt
    19981 gcaaagcgtt gagggcgaaa aagaggccag cgccgcgcgg caggccaagc tggcgctgga
    20041 tatcgccaac aaaacgctac cgctgtttcg ccatgtcaat agcgagtcat tacgcaaggt
    20101 ctgcgaaatt atccgcgatg acattcacgc cgatgcggtg gcgattacta ataccgatca
    20161 tgtgctggcc tatgttggcg tgggtgaaca taactatcag aatggcgatg acttcattag
    20221 cccgactacc cgtcaggcga tgaattacgg aaaaatcatc attaaaaaca atgatgaagc
    20281 ccaccgcaca ccagagattc attccatgct ggtgatccca ttgtgggaga aaggcgttgt
    20341 gaccggaacg ctgaaaattt actactgcca cgcgcatcag ataacctcgt cattacagga
    20401 aatggcggtt ggtctgtcgc aaatcatctc cacgcaactg gaggtttcac gcgccgagca
    20461 gctacgtgaa atggcaaata aggcagagct tcgcgccctg caaagcaaaa ttaatcccca
    20521 ttttctgttt aacgctctga acgctatttc atcgtcaatc cgtctgaatc cggataccgc
    20581 tcgccagttg atctttaatc tgtcgcgtta tctgcgctat aacattgaat taaaagacga
    20641 tgagcaaatc gatatcaaaa aagagctgta tcaaattaaa gactatattg ccattgagca
    20701 ggcccgcttt ggtgacaagc tgacggttat ctatgatatt gatgaagagg tgaattgctg
    20761 cattcccagc ctgctgatcc agccgttggt ggagaacgcc attgtccacg gtattcagcc
    20821 ttgcaaaggt aaaggcgttg tcaccatcag cgttgcagag tgcggaaatc gggtacgcat
    20881 tgcggtgcga gataccggca cggcatcgat ccaaaggtga ttgagcgggt cgaagcgaat
    20941 gaaatgccgg gcaataaaat tggcctgctg aatgtccatc atcgcgtgaa gttattgtat
    21001 ggcgaggggc tgcatatccg ccgcctggag ccggggacgg aaattgcgtt ttacattcct
    21061 aaccaacgca ccccagtcgc ctcacaggct acgttattgc tttgagccgg agtgatattg
    21121 tgaaagtcat cattgttgaa gacgaattcc tggcacaaca ggaactgagc tggctaatta
    21181 aagagcacag ccagatggag attgtcggca cctttgacga cggtctggac gtgttgaagt
    21241 ttttgcagca taaccgcgtc gacgccattt ttctggatat caatattccg tcgctggatg
    21301 gcgtgttgct ggcgcaaaac atcagccagt tcgcccataa accgtttatt gtgttcatca
    21361 ccgcgtggaa agaacatgcg gtagaagcgt ttgaactgga ggcgtttgac tacattctca
    21421 aaccgtatca ggagtcacgt attaccggga tgctgcaaaa actggaagcg gcctggcaac
    21481 aacagcagac cagcagtacg cctgccgcga cggtaacgcg tgagaatgac accattaatc
    21541 tggtgaaaga tgagcgaata atcgtcacgc caattaacga tatctattac gccgaagcgc
    21601 acgagaaaat gacctttgtc tatacgcggc gtgaatccta cgtaatgccg atgaacatta
    21661 ccgaattttg cagcaaactg ccgccgtcgc attttttccg ctgccatcgc tcattttgtg
    21721 tcaatctgaa caaaatacgc gaaatcgaac cgtggtttaa taacacctac attctgcgac
    21781 tgaaagatct ggattttgaa gtgccggtca gccgcagcaa agtgaaagaa tttcgccagt
    21841 taatgcatct ttaatcggga actttcatga aagcaccagg cttgcccgcc gatcaacaat
    21901 ttttcgccga tctgttcagc ggcctggtgc ttaactcgca actactgggg cgcgtctggt
    21961 ttgccagcca gcctgcctcg ttaccggtgg gcagtttatg tattgatttt ccccgtctgg
    22021 atatcgtgct gcgcggcgaa tacggcaacc tgctggaagc aaagcagcag cgaatggtgg
    22081 aaggagaaat gctgtttatt ccggcacgcg cggctaattt accgatcaac aacaaaccgg
    22141 tgatgctgtt aagcctggtg ttcgcaccga cctggcttgg gttatcgttt tacgatagcc
    22201 gcaccacgtc gttgttgcat cctgctcgcc agatccagct tcccagcctg caacgcggtg
    22261 aaggtgaagc gatgcttacc gccctcactc atcttagccg ttcgccgctg gagcagaata
    22321 tcattcagcc actggtgtta agtttgctgc atctttgccg caacgtggtg aatatgccgc
    22381 cgggtaattc gcaaccgcgc ggcgattttc tctatcacag catttgtaac tgggttcagg
    22441 ataattatac ccagccgctc acccgcgaga gcgtggcgca gttttttaat atcacgccca
    22501 atcatctgtc aaaactgttt gctcagcatg gaacgatgcg ttttatcgag tatgtgcgtt
    22561 gggtgcgaat ggcgaaggcg aggatgattt tgcagaaata tcatctgtca attcatgaag
    22621 tggcacagcg ttgcggtttt ccggatagcg actatttttg tcgcgttttc cggcgtcagt
    22681 ttggtctgac gccgggagag tacagcgccc gttttcaggg ctaacgtcag aaggttaatt
    22741 ctgtttccag cagcgtcagg atactttgtg catcttgcgc ggcaaacaac gactggcgga
    22801 agtttttatt caccagttta cgcgccagct gcgagaagac tttcacatga ttcatccctt
    22861 cgttagcacc cagcgtcagc atgatcacca gttcgacttc gcccatttct gactgccagt
    22921 caatcggttt cgccagccgg gcaatgctga tactggaatg acggatccac tgagatttgg
    22981 tgtgcgggat cgctacgcca aaaccaacgc cggtggtaac aatctcttcc cgctgccaga
    23041 catcttcttc cagctcaaac ggatgttcag tgcgcccgtt aacgccgagg ttgccgcaca
    23101 ggaactggat cgcctgctct ttattgctaa aatcctgatc aacaaagata ttctccagcg
    23161 ccagcagtgg gcgaacgtct tcttccggcg taaaggcggt gagtaacgct tcaatttcct
    23221 gcgcactgcg gcattcacat gcctgacgcg ccagttcccg acacgcctcg ctatccagtt
    23281 gacgaagctg gcttttcacc gccggaatac gcgggctact catactcagc tcatccaggc
    23341 ccagcccaag cagtagcggc agataacggc tttcaccgcc cagttcaccg caaatgccta
    23401 cccatttgcc ccgctgatgc gcagtggtaa ctatttgctg caacatgcgc aggaacgatg
    23461 gcgtaatcgg gttatatagc ggcgatacgc gcgggttatt acgatcgacc gcatacagat
    23521 actgggtcat atcgttggag ccgatactga agaaatcgac ctcatcgcag aagtggtcga
    23581 tgatgtagca caccgacggc acttccacca tgatcccaag cgtaatcgtc tctgcatgac
    23641 gcaggccatc gcgcttaagc tcaacgatcg ctttttggat ctcgcccttc acccataaga
    23701 tctgatcgag actgtggacc atcgggatca tcaactgggc gttgccaaaa ctggcggcgc
    23761 gcaaaatcgc ccgtagttga gtgcggaaca ggccagcaaa ttccgggtaa atacgtaccg
    23821 cgcgatagcc gaggaacggg ttctcttcct ggggaatatt cagataagga atgcttttat
    23881 cgccaccgat gtccattgtg cggaagataa tcggcttgtc gcccgctgcc agtagcacct
    23941 gctggtaggc ttcaaattgc tcctgctcgt ccggcgcgct gtcgcgatcc atatagagca
    24001 tttcagtacg gaacagtcca acgccttccg cgccgttggc aaacgcgcct ggcgcttcca
    24061 gagcggtgcc gatattcgcc gcaatatcaa tacgcttgtt atcacgggaa taggccagct
    24121 gcgcggcggc ctgcgcctgt tgtttttggc gtttatccgc cagcgtctgt gcgacctgat
    24181 aataaccgct caccgcgtca ttcgggttaa tcgccagcac gccgcactgg gcgtcaagca
    24241 ccgccggttg cccggcataa cgggcaatcg catccagcgg caggccgctc agtaccggga
    24301 tcgccgaggc gcgggccaga atcagggtat gcgaggtgcg cccagttttc tccagaatca
    24361 tgcctgcaag attttttaaa tcgaggctca aaaactggct tggggttaaa tcctcggcta
    24421 ccagaatagt cggtttttcc agcaccagat tgttgcgcgg cttcagttcc ggccaggtga
    24481 tatgcagcaa ctgttcgcta atatcgcgaa tgtcgctaac acgttcgcgc agataatcac
    24541 tggcagaagc agaaagtttg gcgcaaacct gctccatatt gctgatgatc gccgccccca
    24601 gcccttgatg ctgttctgtc atcagacgac ggatattgcc cgcgaattca tcatcctgaa
    24661 tcagcgacaa atgggcgcta aggatagttt tgctttcgcc gtcactctca cgcaattgct
    24721 ggttcagttg ctcggcaagc gttgcaaggc tgtgctccag ccgggtggaa tcttgcgcac
    24781 tggcggggat tgcccgataa ctgtcgaggc tgtcgctttg taacagggtc agcgtaccca
    24841 cgccgacgct gcttgccagc acattgccgt acagtaaatc cgggttcagg cggcttaatg
    24901 aacgcggcag cggatgcgcc gtcagttctg cctgcgttgg ctggatgctg tcgctgtcga
    24961 taaagcgcac ctggatgtac tcttccaaca cgcgctgcgc ctgctcttca tcgctgccgc
    25021 taatgttcag gctgcaactg tcattaaata aagtaccggt gccaatcagc gccagcgagc
    25081 ttttcgcatc ggcctttgcg ttctggcgat ggttgataaa ggtgatttcg ctttgccact
    25141 ggctgcactg ttctttaagt tcccacgccg gacgagcatg aagaccgttt ggcaaaggac
    25201 agagaaattg aatcgttaac ataaccgctc cgggttaatc aggagatcat ctgaaatccg
    25261 tcagttgaac aaccgtctca cgcgtaagac gtttaataag ggcagataaa agctgctgca
    25321 tctgcaaaat gtcacggcaa tcggcaatcg atgcggcgca atgtccgtgg cgggttgctg
    25381 gccccatcac cactgtaggc acgccggtgc cggttaagtg caccgctccg ccgtccgtgc
    25441 cgccgttgct gaacatatct gcctgtaacg gcacgccaat ttctgccgcc acggtttcga
    25501 tccacgcggt aagttttggc ggcgcaatca gcgacttgtc gcttaacacc agcatcggcc
    25561 cgttacaaat ctgacgatgg ttagccgcgc cataatcaaa gtttttcgcc cagcaggcgg
    25621 tatcaagcac aatggcgaca tccggcgaca ccgcgcgggt ggcagtttgc ccgccgcgta
    25681 cccccacctc ttcgctggaa ctggccacca gccacacttc cgcaggcagt tcagcgctgt
    25741 gtagttcgcg cagtaacgtc accagcaggt agcaaccgag gcggtcatca aaggctttcc
    25801 ccatcacccg ctggtgaggg agaacctgaa aagttgtatc aaaggtgacg cgatcgcctg
    25861 gacgaattcc cgcctgcatc acttcgtcat acgagcgcgt accaatgtcc acgcgcatgg
    25921 cgctgacgtc attcccctgc cggtcgccgt caagcaggcc tggaattttg cactcttcac
    25981 gggtggtgat gcgcaccggc tgcagctggc gggcagccat gcgcacgttg ccaaccggca
    26041 gcacatcaat tgccccttcg cgggagatgc tgcgcaccat aaatcccact tcgtccatat
    26101 gggcgcagat catcaccttc ggccctgtcg attcattgag gcggatcagc accgatccca
    26161 gaccatcaaa tcgcacttct ttttgcaggc gatccgcttc ttccagcagg atctgccgca
    26221 cttcctgttc cgaggaggcg atcgcatctg cctcgctcaa cgcttttaat agcgataaat
    26281 ccattatgcc tctcccgtga gcaacactgt tttcggcatg gcgtagagca cttctgcgcc
    26341 ttgcggggta accagcacaa catcttcgat gcgcacgccc ccttgccctg gcaaataaat
    26401 ccccggctcc acggtcagca acatgcctgg ctgtagcgtc gtggtgtccc gcggtgaaaa
    26461 acgcggatct tcatggactt caatgccgat agcgtgacca gtgttatgac cgaaatagtc
    26521 gccaaatcca gcctcggtaa tgacccggcg cgcggcgtcg tcaatctgct ggcagcgcac
    26581 gccggggcga attgcagaga ttgccgcgag ctgtgcctgc aggacaatct gatagacgtc
    26641 aaacagcggg tgagattcgg cgctcacccc ttcgccattc accagcaagg tgcgcgtcat
    26701 atcagagcag tagccctgat acagcgcacc aaaatcgaga gtgacaaact cgcccgctgc
    26761 aacaatcttg tcgctggctt tgccgtgcgg cagcaccccg cgccagccgc tggcgacaat
    26821 ggtgtcaaaa gaggcttttt ctgcgccctg ctggcgcata aaccactcca gttcagcggc
    26881 tatctcgcgc tcgctcatcc ccgcctgaat aaagcggcga atatgctccg ctccgcgatc
    26941 ggcaatccca caggcgaggc ggattatctc cacctcctct ggcgttttga tttgccgcag
    27001 cacatccggc gtggcgctaa ccagtttcgc attgagttca gacttccagc ggtgcgcggt
    27061 ttcccaactc acctgctggc cttcaaaacc gagcgtttgc aactgctcat cggcaatgat
    27121 ttgattgacg atagtggtaa gcgtgttcgt cgcgtcaagc aaatgcagct ggtagccttg
    27181 cgcacgggct tccacctcaa cgaaatagct cgaatctacc agaatgtgcg cactttcacg
    27241 gctaataacc acatagcctg atccggtgga gatccccaga tgcggctgtt tgttctgccg
    27301 tgaggagaga agcactgcat ccagttgttg ctccttaagc cagtcgcgca gcgaagcgag
    27361 taatgtcatc tggattttcc ttcttacagg ctatcgatta acaatttgcc tttacggaac
    27421 atcatcagac gcaggaacac caccatcagc gcagttatga ctgcccccag cgcgatcccc
    27481 gccatataga cgccaaggtt agtgaccagc ggccatgccc agatagcgga ttccgggaac
    27541 cattgcactg cgcccagcca gacagcggcg gttgagccga caatcgcgcc gaccatatac
    27601 gacggaatgg cggtgatggg gctttccagc gcaaacggaa tcgcaccctc actaatcccc
    27661 ataaaggcaa ggaacatcgc ggtcttaccc tgcggataaa gttgagcgtt gaacaggcgt
    27721 ttgccggtta aacgacggtc aataatggtc gccagcccca gaccaatcgg aggaataacg
    27781 atagcgatag aacgagcggt gaccggtaat acgtggtcag tggtaaagct gaaggcaacg
    27841 aaacctgcgg ctttgttgat cgggccacca aggtcgattg ctgtcgccgc ggcgatcccc
    27901 attgcgtaca tcagcgcgcc cttctcacct gcggcagtca gcacggtacg gataacgccg
    27961 ttgatccagc cgccaaaggg ggtaatgacg tagtacatcg ccagcatgac aaaaatggcg
    28021 gaaagaatcg gtagcaaaaa tgtggttttg aacgctaaca ggaaatccgg taactggatt
    28081 ttctggttca tccacttcac aaggtaaccc gcgacgatag aaataatcag cgcaccaatg
    28141 aaggtggacg gcaccggcga agaggtcgcc cattgcatag tgctgggatc gaagttcagc
    28201 agttgcgtcg gctgggtaga catcaagccg ccgataaaac cagccgggaa cgccagtttg
    28261 ccaccgatag agttcgccac aaaagcagca aacatcggaa tggcaaaacc aaacaacacg
    28321 ccgccgaagg actgcgacag ccaggcaaat ttcagtaagg agaggtcgaa accggagaat
    28381 ttcccgctat taagagcatc catgatgccg atctcagcag gaattttaag ccagctataa
    28441 gcaatcagct ggctgaaagc gaggatcaca ccgcccataa ttaaggtcgg caccatgcgt
    28501 gaaatccctg acatcacatg ctgcgggagt tcaccccaga agctgctttt agaggcctgc
    28561 ggattcttaa ctgccgctgc cgcaccggat gcgccaggca caacggttgc actgcgtttt
    28621 ttaatggcca taacaattat ccctgtcgaa tattactgtt gttcagaggc aatcatctct
    28681 tcgatttctt tgatgatgcc cgcagcgttt ttaattgcgt cctgcaaagt gatttcataa
    28741 acgtcgcgtg attcgaaacg ctcattatct tccggggtaa ctgccacgga gtggatgatg
    28801 atggttgctt cggcgatatc ctgcgccgtc aggcgattct ggataccgtc cgcgccctgg
    28861 gtttcaattt tcacttcata accggcttct accgccgctt cttccaacgc ctgagcggcc
    28921 ataaaggtgt gagccaggcc cattgggcag gcacataagg caatcagttt cttactcatc
    28981 gttaattcct cattcagtga tagttgacaa gtgaaattat gaacgtgcaa aacaaatcgc
    29041 cgttaccaga caaataatgc atttactgga gaattaatcc cgctgtacgg aagtgtgacg
    29101 caggtcggcc ttgtgagggg acacaaagaa aaattgaatt aactgtatga tttaaacgat
    29161 tatcgggaga gttacctccc gatataacag gaaggattta cagaatgtgc cctaaggtct
    29221 ggcgtaaatg tgcaccggaa ccgagaagac ccggattgtc atggacgatg agatacaccg
    29281 gaatatcatg gacatattct ttaaagcgcc ctttatcttc aaatgcagca cggaaaccag
    29341 aggctttgaa gaactcaagg aagcgcggca cgataccgcc cgcaataaac acgccgccaa
    29401 atgtcccgag attgagcgcc agattgccgc caaaacggcc cataatgacg caaaacagcg
    29461 acaacgcgcg gcggcaatcg gtacagctgt cagccagcgc gcgttcggta atatcttttg
    29521 gcttgagatt ttctggcagg cggttgtcag ctttcacaat tgcgcgatac aaattcacca
    29581 gcccagggcc agaaagcacg cgctccgccg aaacatgacc aatttccgca cgcaatattt
    29641 cgaggataat ggcctcttct tcactattcg gcgcaaaatc aacgtgaccg ccttcgcccg
    29701 gcaagcttac ccaacgctta tcgacatgga ccagatgcgc aaccccaagc cctgttccgg
    29761 caccgtaaac cgcaataggt ttaccttcta ccggttctgc gccaccaaac tgaatcagat
    29821 gctctttttt cagcatcggg atcgccatcg atacagcggt aaaatcgtta ataatttcca
    29881 gatggctaaa accgagattc tttttcattt cggcaattga gaacgcccag gtatggttgg
    29941 tcattgccac ccagtcaccg gtaattgggc aagcgatggc aatacagccg tctttcacct
    30001 cgaccttatg ttcttcaaga taaacgcgaa tgaccgcttc gaggctgggg taatcaagcc
    30061 ctgaataggt cttagcctgc gagatttcac cactggcaat atcacacaga gcaagacgtg
    30121 cgttggtgcc gcccacatca ccgactaatg catactttat cattcttcaa ctgctccgct
    30181 aaagtcaaaa taattctttc tcacactgta aatacctggg ggcataacaa caacgccgga
    30241 aaggcaggct ccctgtaaat atcgatctgg gtcacacatt tactttatcg tttcagcacc
    30301 aattgcagcg atgccttttt gcaagctggg caaactaagt atctgacccc gcataaggaa
    30361 tagaacatgc tccatccgcg agccagaacc atgttgttat tatcgctccc cgccgtggca
    30421 attgggattg cgtccagtct tattctgatt gtggtgatga aaatcgcctc ggtattacag
    30481 aatttgctct ggcaacgact gccgggaact ctggggatag cccaggattc acctctctgg
    30541 atcatcggtg tattaacgct aacgggtatt gcggtggggt tggttatccg tttcagccag
    30601 ggtcatgccg gaccagaccc cgcctgtgaa ccgctgatcg gcgcaccagt tccgccctct
    30661 gcgctaccag gacttatcgt agcattaatt ctcggtcttg ctggcggcgt cagcctgggg
    30721 ccggaacatc cgatcatgac catcaatatc gcccttgccg tcgccattgg cgctcgtctg
    30781 ttaccgcgcg tcaaccgaat ggagtggact attttagcct ctgccggaac catcggcgcg
    30841 ctgtttggca cacctgttgc agcggcgttg atattttcgc aaaccttaaa tggcagtagt
    30901 gaagttccgc tatgggatcg tctctttgcg ccgttaatgg cggcagcagc tggtgcacta
    30961 actaccggat tatttttcca tccccatttt tcactgccca ttgcgcatta cggacagatg
    31021 gaaatgaccg atattctcag cggtgcaatt gtcgcggcga ttgccatcgc agcagggatg
    31081 gtcgccgtat ggtgcttacc acggttgcac gcgatgatga atcaaatgaa aaatccggtg
    31141 ctcgtgctgg gtattggcgg atttattctc ggtattctgg gggttattgg tggaccagtt
    31201 tcgctgttta aagggctgga tgagatgcag cagatggtgg caaatcaggc tttcagcacc
    31261 agcgattact ttttgctggc ggtaattaaa cttgccgccc tggtcgttgc tgccgccagt
    31321 ggctttcgcg gtgggcgaat cttcccggca gtgtttgtcg gcgtggcatt agggttgatg
    31381 ctgcatgagc acgtacccgc cgtaccagcg gcaataaccg tttcttgcgc tattctcggc
    31441 atcgtgctgg tggtaacacg cgatggctgg ttaagtcttt ttatggcggc ggtcgttgta
    31501 cccaatacca cattgctacc gctgctctgt atcgtcatgc ttccggcatg gctgttatta
    31561 gcaggtaagc cgatgatgat ggtcaatcgt ccgaagcaac agccacccca cgataacgtt
    31621 tagcaaaaaa tgctctcctt tatgatttaa gagttatggc gatgatacgg ggctttacct
    31681 ccccgtaata ttgcgttaac aggccgctga cggcttcgat cgtgaaggag aataacaatg
    31741 ttcaggtcac tgtttctggc ggccgccctg atggcattta ccccgcttgc agcaaacgca
    31801 ggtgaaatca ccctactgcc atcaatcaaa ttacaaattg gcgatcgcga tcattacggt
    31861 aattactggg acggtggtca ctggcgcgac cgtgactact ggcatcgcaa ttatgagtgg
    31921 cgcaaaaacc gttggtggcg tcatgataat ggctatcatc gtggctggga taagcgtaaa
    31981 gcgtatgagc gtagctatcg tgaaggctgg cgcgatcgtg acgatcatcg cggaaaaggc
    32041 cgcggacatg gacaccgcca ttaaacacat tcgccaatgg agcacaatgc ctgatgcgac
    32101 gctagcgcgt cttatcaggc ctacaaaccg gatctttaac gcgtagggcg gataaggcgt
    32161 ttacgccgca tccgccaata gtgccagatg cgacgctcat tcaactacaa tcccagcgcc
    32221 gtccccacca acaaccagat attcagcgcc acgaccagca ctacaatcac ccagcctgtc
    32281 tgttttacgc gtttgctgtt caccagatcg cccatcaact tgctgtcact ggtgaaaatc
    32341 agcagtggaa ccagcgccag ggcgatacca aaacttaaca gcacctgact cataaccaga
    32401 atccgtgtcg gatctaatcc catcagaatg acaataaatg acggcagcat ggtgactgta
    32461 cgacgcaccc acagcgggat atggaagcga atgaacccct gcatcaccac ctgccctgcc
    32521 agtgtcccca ctaccgttga ggacagtccg gcagcaacca gacttaaccc aaagaccgtt
    32581 gccgcagcat ggcttaacag cggttgcagc gtcagataag cctcatcaag atcggcaaca
    32641 ccagtatgac cagaaaagtg gaacgccgcc gcagctgtag ccatcatcgc cagattgaca
    32701 aaaccggcaa tagtcatggc gatagccaca tcccatttgg tggcggaata acgttgttga
    32761 cgcgaaccgc catgtaaatg ctgagtgagc gaggagtgca aataaatcac atgcggcata
    32821 atcgtcgccc ctaacacgcc tgctgccagg aagaccgctt ccgaagtagg taaactcggg
    32881 agcaccattc ctttacccag ctgcgccagg ttaggctggg agaaaatcaa ctcgacaatg
    32941 taagccgcgg caacaaacaa cagtaacccg ccaatcactt tctccagcgt tttttgcccg
    33001 cgacgttgca gcattaaaat caggaaagtc gcgatccccg tcagcaccgc gccctgcaac
    33061 aacgaaacac caagaatgag tttaaaaccg atcgccgcac caataaattc cgccaggtcg
    33121 gttgccatcg caataatttc tgcctgaacc caatagaacc acactacggg acgcggatag
    33181 tgatcgcgaa tctgctccgc cagattttta ccggtggcaa tccctagttt ggcagagagg
    33241 atctgaatca gcatcgccat caggttggcc caaacgacaa cccacagtag ctgatagccg
    33301 aagctggcac ccgcctgaat attggtcgca aagttaccgg gatcgatata accaatcgcc
    33361 gcaatgaacg caggtcccat taatgcgagc ctcatcttgc gcgccgcccg tccgctgcta
    33421 ctctcaacgc gatagttcgt catcttgtgc ctctaaaaca tagcctttgc tatgtttcat
    33481 gctatgataa acgagaatga ttatcaaatt catttaaatg gattgtggtg atttctgtga
    33541 tagaccagga ttatgactac gaaaagattg atgacttgaa tgttatggtt atgtttaatg
    33601 ttagcacatt tacataactt tcagcttcca tacacaacat agcagaaatg tatgacagat
    33661 cactattttt gaagcctgtc acaggacgtc attatagtgt gtgtcagatc tcgttttcct
    33721 taaccatgtt acatagaatg tgcacggaaa tttaacctgc ctcatatttg gagcaaatat
    33781 ggaccgcgtc cttcattttg tactggcact tgccgttgtt gcgattctcg cactgctggt
    33841 aagcagcgac cgcaaaaaaa ttcgtatccg ttatgttatt caactgcttg ttatcgaagt
    33901 gttactggcg tggttcttcc tgaactccga cgttggttta ggcttcgtga aaggcttctc
    33961 cgaaatgttc gaaaaactgc tcggatttgc caacgaaggg actaacttcg tctttggtag
    34021 catgaatgat caaggcctgg cattcttctt cctgaaagtg ctgtgcccaa tcgtctttat
    34081 ctctgcactg atcggtattc tccagcacat tcgcgtgttg ccggtgatca tccgcgcaat
    34141 tggtttcctg ctctccaaag tcaacggcat gggcaaactg gaatccttta acgccgtcag
    34201 ctccctgatt ctgggtcagt ctgaaaactt tattgcctat aaagatatcc tcggcaaaat
    34261 ctcccgtaat cgtatgtaca ccatggctgc cacggcaatg tccaccgtgt cgatgtccat
    34321 cgttggtgca tacatgacca tgctggaacc gaaatacgtc gttgctgcgc tggtattgaa
    34381 catgttcagc acctttatcg tgctgtcgct gatcaaccct taccgtgttg atgccagtga
    34441 agaaaacatt cagatgtcca acctgcacga aggtcagagc ttcttcgaaa tgctgggtga
    34501 atacattctg gcaggtttca aagttgccat tatcgttgcc gcgatgctga tcggctttat
    34561 cgccctgatc gctgcgctga acgcactgtt tgccaccgtg actggctggt ttggctacag
    34621 catctccttc cagggcattc tgggctacat cttctatccg attgcatggg tgatgggtgt
    34681 tccttccagt gaagcactgc aagtgggcag tatcatggcg accaaactgg tttccaacga
    34741 gttcgttgcg atgatggatc tgcagaaaat tgcttccacg ctctctccgc gtgctgaagg
    34801 catcatctct gtgttcctgg tttccttcgc taacttctct tcaatcggga ttatcgcagg
    34861 tgcagttaaa ggcctgaatg aagagcaagg taacgtggtt tctcgcttcg gtctgaaact
    34921 ggtttacggc tctaccctgg tgagtgtgct gtctgcgtca atcgcagcac tggtgctgta
    34981 agaccataca taaaaaagcc ggggataatt cccctaatgc cagtcagtta agcaactgac
    35041 tggctctttt tcggggctgt ggggtatttc cagggcctct cctttaccac tctcgggaag
    35101 gcccttcccc ttctcgtcgg ta
//
LOCUS       DAJTMC010000023        35065 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.023, whole genome shotgun sequence.
ACCESSION   DAJTMC010000023 DAJTMC010000000
VERSION     DAJTMC010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35065
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.023"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(<1..334)
                     /locus_tag="OO130_000990"
     CDS             complement(<1..334)
                     /locus_tag="OO130_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCR5364488.1"
                     /translation="MNKEEQYLLFALSAPMEILNQGCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWHRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGE"
     gene            complement(415..1842)
                     /locus_tag="OO130_000991"
     CDS             complement(415..1842)
                     /locus_tag="OO130_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HCR5364489.1"
                     /translation="MKNCWKILDIEETTDVDIIRRAYLALLPSFHPETEPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSAGRLDIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCD
                     YWREYPSTQADALILQWCRQHRVDYYPLVVMMIEARELVNDQGKQLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHLLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTEKQTVEPEPQPQPMPVDDGGPGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(1839..2546)
                     /locus_tag="OO130_000992"
     CDS             complement(1839..2546)
                     /locus_tag="OO130_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCR5364490.1"
                     /translation="MDMGSQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRTIHRMIENGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQP
                     LADYPTCPETLKDMSDL"
     gene            2709..3686
                     /locus_tag="OO130_000993"
     CDS             2709..3686
                     /locus_tag="OO130_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HCR5364491.1"
                     /translation="MIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEHAIFWLKKTALQGHTFASN
                     ALGWILDRGEDPNYKEAVVWYQIAAESGMSYAQNNLGWMYRNGNGVAKDYALAFFWYK
                     QAALQGHSDAQNNLADLYEDGKGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEDLL"
     gene            complement(3756..4238)
                     /locus_tag="OO130_000994"
     CDS             complement(3756..4238)
                     /locus_tag="OO130_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HCR5364492.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            4473..7055
                     /gene="leuS"
                     /locus_tag="OO130_000995"
     CDS             4473..7055
                     /gene="leuS"
                     /locus_tag="OO130_000995"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HCR5364493.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKS
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            7070..7651
                     /gene="lptE"
                     /locus_tag="OO130_000996"
     CDS             7070..7651
                     /gene="lptE"
                     /locus_tag="OO130_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HCR5364494.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            7651..8682
                     /gene="holA"
                     /locus_tag="OO130_000997"
     CDS             7651..8682
                     /gene="holA"
                     /locus_tag="OO130_000997"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HCR5364495.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRTLHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQTQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            8684..9325
                     /gene="nadD"
                     /locus_tag="OO130_000998"
     CDS             8684..9325
                     /gene="nadD"
                     /locus_tag="OO130_000998"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HCR5364496.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNQED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            9349..9957
                     /locus_tag="OO130_000999"
     CDS             9349..9957
                     /locus_tag="OO130_000999"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HCR5364497.1"
                     /translation="MRLWLIRHGETQANIDGLYSGHAPPLTARGIEQAQNLHTLLHGV
                     SFDLVLCSELERAQHTARLVLSDRQLPVQIIPELNEMFFGDWEMRHHRDLMQEDAENY
                     SAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEFQHYQNILVVSHQGVLSLLIARLI
                     GMPAEAMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVDNA"
     gene            10217..10534
                     /gene="rsfS"
                     /locus_tag="OO130_001000"
                     /gene_synonym="rsfA"
     CDS             10217..10534
                     /gene="rsfS"
                     /locus_tag="OO130_001000"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HCR5364498.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            10538..11005
                     /gene="rlmH"
                     /locus_tag="OO130_001001"
     CDS             10538..11005
                     /gene="rlmH"
                     /locus_tag="OO130_001001"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HCR5364499.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            11036..12937
                     /gene="mrdA"
                     /locus_tag="OO130_001002"
     CDS             11036..12937
                     /gene="mrdA"
                     /locus_tag="OO130_001002"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HCR5364500.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTTHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            12940..14052
                     /gene="mrdB"
                     /locus_tag="OO130_001003"
                     /gene_synonym="rodA"
     CDS             12940..14052
                     /gene="mrdB"
                     /locus_tag="OO130_001003"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HCR5364501.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            14063..15151
                     /gene="rlpA"
                     /locus_tag="OO130_001004"
     CDS             14063..15151
                     /gene="rlpA"
                     /locus_tag="OO130_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HCR5364502.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            15291..16502
                     /gene="dacA"
                     /locus_tag="OO130_001005"
     CDS             15291..16502
                     /gene="dacA"
                     /locus_tag="OO130_001005"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HCR5364503.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDMYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            16613..16876
                     /locus_tag="OO130_001006"
     CDS             16613..16876
                     /locus_tag="OO130_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HCR5364504.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVEILYEELGKIDIVRMVL"
     gene            16977..17618
                     /gene="lipB"
                     /locus_tag="OO130_001007"
     CDS             16977..17618
                     /gene="lipB"
                     /locus_tag="OO130_001007"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HCR5364505.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            17877..18830
                     /locus_tag="OO130_001008"
     CDS             17877..18830
                     /locus_tag="OO130_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HCR5364506.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIALNLLTIFEAVYVHK
                     GIVNAAKVLNLPPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHVLLL
                     PEEQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNKEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            19039..20004
                     /gene="lipA"
                     /locus_tag="OO130_001009"
     CDS             19039..20004
                     /gene="lipA"
                     /locus_tag="OO130_001009"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HCR5364507.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVAIEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(20105..20308)
                     /gene="tatE"
                     /locus_tag="OO130_001010"
     CDS             complement(20105..20308)
                     /gene="tatE"
                     /locus_tag="OO130_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HCR5364508.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(20437..21225)
                     /locus_tag="OO130_001011"
     CDS             complement(20437..21225)
                     /locus_tag="OO130_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HCR5364509.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENAVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRRVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            21318..21701
                     /gene="crcB"
                     /locus_tag="OO130_001012"
     CDS             21318..21701
                     /gene="crcB"
                     /locus_tag="OO130_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HCR5364510.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLTANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(21756..21965)
                     /gene="cspE"
                     /locus_tag="OO130_001013"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(21756..21965)
                     /gene="cspE"
                     /locus_tag="OO130_001013"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCR5364511.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(22140..22700)
                     /gene="pagP"
                     /locus_tag="OO130_001014"
     CDS             complement(22140..22700)
                     /gene="pagP"
                     /locus_tag="OO130_001014"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HCR5364512.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADEWMTTFRENIAPTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
     gene            23289..24674
                     /gene="dcuC"
                     /locus_tag="OO130_001015"
     CDS             23289..24674
                     /gene="dcuC"
                     /locus_tag="OO130_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HCR5364513.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            complement(24715..25395)
                     /gene="dpiA"
                     /locus_tag="OO130_001016"
                     /gene_synonym="citB"
     CDS             complement(24715..25395)
                     /gene="dpiA"
                     /locus_tag="OO130_001016"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HCR5364514.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISRTSARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(25364..27021)
                     /gene="dpiB"
                     /locus_tag="OO130_001017"
                     /pseudo
     CDS             complement(25364..27021)
                     /gene="dpiB"
                     /locus_tag="OO130_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
     gene            27370..28428
                     /gene="citC"
                     /locus_tag="OO130_001018"
     CDS             27370..28428
                     /gene="citC"
                     /locus_tag="OO130_001018"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HCR5364515.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLKTPTISAPPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            28443..28739
                     /gene="citD"
                     /locus_tag="OO130_001019"
     CDS             28443..28739
                     /gene="citD"
                     /locus_tag="OO130_001019"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HCR5364516.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGTLDCILRARLEALLARASGIPTLPWEDCQ"
     gene            28736..29644
                     /gene="citE"
                     /locus_tag="OO130_001020"
     CDS             28736..29644
                     /gene="citE"
                     /locus_tag="OO130_001020"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HCR5364517.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            29655..31187
                     /gene="citF"
                     /locus_tag="OO130_001021"
     CDS             29655..31187
                     /gene="citF"
                     /locus_tag="OO130_001021"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HCR5364518.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIHRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQEQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPTR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            31191..31741
                     /gene="citX"
                     /locus_tag="OO130_001022"
                     /pseudo
     CDS             31191..31741
                     /gene="citX"
                     /locus_tag="OO130_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
     gene            31716..32593
                     /gene="citG"
                     /locus_tag="OO130_001023"
                     /pseudo
     CDS             31716..32593
                     /gene="citG"
                     /locus_tag="OO130_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308679.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
     gene            32644..34107
                     /gene="citT"
                     /locus_tag="OO130_001024"
     CDS             32644..34107
                     /gene="citT"
                     /locus_tag="OO130_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HCR5364519.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPNGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            34218..35024
                     /gene="rna"
                     /locus_tag="OO130_001025"
     CDS             34218..35024
                     /gene="rna"
                     /locus_tag="OO130_001025"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HCR5364520.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEAHASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPSNCGKTFVIDKVGY"
BASE COUNT         8732 a         8941 c         8666 g         8726 t
ORIGIN      
        1 cttccccaca gcacatagcg gtttgggcaa cgaattgggt ataaatcaac cctctctccg
       61 agccaccagc aattaatgct ttccactctt caggggaaga gcgatgccat gtcagatata
      121 agccagctaa atcattggca tgaccatcat cagtcatctg ataaatagtc tgaattaaat
      181 catcacgatt atttattccc caggatgatg agagatcgaa ttcttttata ccggtataca
      241 tttttggcga gtcatgcgcg ggtttgcaac cctgattaag tatttccatg ggcgcagaaa
      301 gcgcaaaaag aagatattgt tcttccttat tcatttgtta ataatccctt aagtgcatta
      361 actgactact tttttatatt acttgccggg ctttccgcac attaacgtcg actgtcaccc
      421 gaacagatgg agtattttcc ctatcaaacc ggcaaagata aaaatgtaga aaataatttt
      481 gatgacgccc agacagcccg gcccaccatc gtcaacgggc attggctgcg gttgcggctc
      541 cggttcaaca gtttgcttct cggtaaaatt cgccgcctga attaataatc gacgaataat
      601 taatgcttcc agtggacaac gatcttctgc atcaagacgg cttacgatcg gcattaatga
      661 ttcttctgtc ggttggctgg cctgcactaa ttgtttggca actaaatata atggccataa
      721 gagatgttct gtatcacgcg tgagtgagat ccgcggctta cgtcctttgt gcaaaaccat
      781 ctcgaccagt gaacgcccca gcgcagagag tttttcatcg ctgagtattt cgtataaatg
      841 aaaacgcgta cgggcgctgt cgccggggac ataaagtagc tgtttaccct gatcgttaac
      901 cagttcacga gcttcaatca tcatcaccac taacgggtaa taatcgaccc gatgctgacg
      961 gcaccattgc aatattaaag catccgcctg ggtggaggga tactcgcgcc agtagtcaca
     1021 aagttctgcc agcaagcttt cgccttctcc acaatagacg cgttgcgcgt attcataata
     1081 acgcgcatct tcattatcag gttcagcttc ccgccacgac cgggcgtaat ccagaagttc
     1141 ggggatatcc aggcgacctg cgctatacca gcggagtaaa tttctgtgca gttttgcatc
     1201 atcggggatg atcactgcgc gatgttgcgc taaatacaat gtcagccatt ctggatgcga
     1261 tgaccaaatc cgagcgaaca tttggtaaaa atcgatggtg tcattttgca cggcaacggg
     1321 cagatgcaga atggtctgga agttaaaaac attccccttc gcaatggcat ataaaaagga
     1381 ttccagttct tcttcgtcta tttccccgac gttttcctcc tgcaaccacc gcaatctttc
     1441 tgctaataac accacgcact cgaaggataa atgggcagtg ttcatggcga ttgtgcacag
     1501 cgaccagcgt aattcatcaa tctcttccat cgagcaataa tttaattgct gaatgaatcg
     1561 ctgccaggcg gagggcagaa aacgttcgct atcagaggca agtaacgcgc gaaaggcgag
     1621 cagaatttca tgttctgcta cctcatattc ttctggttgc caaacagatt tagcaggcga
     1681 ctgcgcaatc cgtagcgcct cctcatacgc ctgacgaagt tgtttaaaac cctgcggttc
     1741 ggtttctgga tgaaaggacg gtaacagcgc cagataagcg cggcggataa tatcgacgtc
     1801 agtcgtttcc tctatatcga ggatcttcca gcaatttttc ataagtcgct catatcctta
     1861 agcgtttccg ggcatgtggg gtagtcagcc agtggttgcc aggacaaggt agggtaatac
     1921 gcatcatcac tggcaaataa ttgataaaac atatcattgc cggaatcgtc gtattcttta
     1981 cgcgctaaga attcgcgtaa taaaggcaga tgatcatctt cgggagactg ccaatattgc
     2041 cgaccaaagg tataaccggc aaagtattgc cgccagttac tataataacg tagagcccgt
     2101 agatgaatac gcgactggat ccatagactt tcttcttcac tcaaccagtt attaagtacg
     2161 cccattcgac ttaaaaaacc catccggaca taatcccatg ctttaattcc tccctcacca
     2221 cagcattcgg ctgtgctggc gacaaattgc gcataagcct gaccttgctc atttaattca
     2281 gcaagatagt cacgccattc acatggagaa taacgaaacc agcggtgata aaaccctgcc
     2341 aaccgagcgg catgaccgtt ctcgatcatt cggtgaattg tgcgcagcag gtcatcacgg
     2401 tcatcaattc cccatgagga agaaaggtca aaaaagcatg tacctaaata cattttgggg
     2461 gatgaatgcg aaggtaaaca gcattcattg cgtatttcca tcggagtgga aagtgcaaac
     2521 aatatttttt gcgaccccat gtccatataa tttatcctta aacattcatt taatgaatgt
     2581 aaactacatt tcgagactca gttatttata cttttctgac gcgacataat acgcataaaa
     2641 taaggcttac gcaaaaatgg aacacataaa atataatatg ttttatctac aaccataagg
     2701 aaatgataat gatttttacg tcaagttgct gcgataattt atcaatagat gagattatcg
     2761 aacgtgctga aaaaggcgat tgcgaggctc aatatattgt tgggttttat tataatcgcg
     2821 atagcgcaat tgactctccg gacgacgaaa aggcctttta ctggctgaag ctggccgccg
     2881 agcaaggtca ttgtgaagca caatattcct taggacaaaa gtatactgag gataaaagcc
     2941 gtcataaaga taatgagcat gccatctttt ggctgaaaaa aactgcccta caaggccata
     3001 ctttcgcttc caacgccctt ggctggatac tggatcgtgg agaagacccc aattataaag
     3061 aagcggttgt ctggtatcag atagccgcgg agagcggaat gtcttatgcg caaaataatc
     3121 ttgggtggat gtacagaaat ggcaacggag tcgcaaaaga ctatgcgctg gcatttttct
     3181 ggtacaaaca agctgcatta caaggccata gtgacgcgca aaacaatctg gccgatcttt
     3241 atgaagacgg aaaaggcgtt gctcaaaaca agacactcgc cgcattctgg tatttgaaaa
     3301 gcgcacagca gggtaatcgg cacgcccagt ttcaaattgc atgggattat aacgctggcg
     3361 aaggggtgga ccaggactat aagcaagcga tgtactggta tctgaaggct gccgctcagg
     3421 aaagcgtcgg cgcttacgtc aacatcggtt atatgtataa acacggacaa ggcgttgaga
     3481 aagattatca ggctgccttt gaatggttta cgaaagccgc tgaatgcaat gacgccactg
     3541 cctggtataa cctggccatt atgtatcact acggagaagg aagacctgtc gatctccgac
     3601 aggctctcga cctgtatcgt aaagttcagt catccggaac cagagatgtc agtcaggaaa
     3661 ttcgtgagac tgaagattta ctgtagagat aaaaacaagg gccggggaat ttcattcggc
     3721 ccatattctc tttattgctg tgcgaaatca gactattacg gctcaaacgg gcgtctctgg
     3781 aactggtcat gaccacattt cgggcatagc gtcagcactt ccggtgtgta gatcgggaga
     3841 tggaagtgac atttctcgca gaccagattt cccagcccga ccacttctcc gctgtgataa
     3901 accccatgat gattgaggtc ctggaaaact tcgcgccatt caagctgcgt tttatcggtg
     3961 atatctgcca gctcctgcca taagctttct ttaatcaccc gcataaagac gctgtcagat
     4021 tcttctttca ggctctcttc atagctcatg gcgaactctt ccaggtcacg tcttacagct
     4081 cgcgtcagct catcgacctc ggttcgcgtt aactcccctg tttttattac gcgctcgcgc
     4141 gcctgttcca ccagtgcgtc gatatcacgt tcgccattgc gcaggcgttc gctcagtgac
     4201 gcgaccagtt cacggtaata ttgagcaacc ttgttcatcg tttcatctcc cgggtcgtta
     4261 actgactata ataatagacg ttctttgttc cttgctttgc taatacgccc acaaactctg
     4321 taaattaatg tatgaataat ccgggcatca tttcaacgtc gagtgcgcga aaggctgttt
     4381 tgacgcggac gtttgggcta tgctatgcgg atctgaaaaa ccacctcaac gctacatttg
     4441 tagccgtatt gaaaacagga ccactggctg ccatgcaaga gcaataccgc ccggaagaga
     4501 tagaatccaa agtacagctt cattgggatg agaagcgcac atttgaagta accgaagacg
     4561 agagcaaaga gaagtattac tgcctgtcta tgcttcccta tccttctggt cgactacaca
     4621 tgggccacgt acgtaactac accatcggtg acgtgatcgc ccgctaccag cgtatgctgg
     4681 gcaaaaacgt cctgcagccg atcggctggg acgcgtttgg tctgcctgcg gaaggcgcgg
     4741 cggtgaaaaa caacaccgct ccggcaccgt ggacgtacga caacatcgcg tatatgaaaa
     4801 accagctcaa aatgctgggc tttggttatg actggagccg cgagctggca acctgtacgc
     4861 cggaatacta ccgttgggaa cagaaattct tcaccgagct gtataaaaaa ggcctggtat
     4921 ataagaagac ttctgcggtc aactggtgcc cgaacgacca gaccgtactg gcgaacgaac
     4981 aagttatcga cggctgctgc tggcgctgcg ataccaaagt tgaacgtaaa gagatcccgc
     5041 agtggtttat caaaatcact gcttacgctg acgagctgct caacgatctg gataaactgg
     5101 atcactggcc agacaccgtt aaaagcatgc agcgtaactg gatcggtcgt tccgaaggcg
     5161 tggagatcac cttcaacgtt aacgactatg acaacacgct gaccgtttac actacccgcc
     5221 cggacacctt tatgggttgt acctacctgg cggtagctgc gggtcatccg ctggcgcaga
     5281 aagcggcgga aaataatcct gaactggcgg cctttattga cgaatgccgt aacaccaaag
     5341 ttgccgaagc tgaaatggcg acgatggaga aaaaaggcgt cgatactggc tttaaagctg
     5401 ttcacccatt aacgggcgaa gaaattcccg tttgggcagc aaacttcgta ttgatggagt
     5461 acggcacggg cgcagttatg gcggttccgg gtcacgacca gcgcgactac gagtttgcct
     5521 ctaaatacgg cctgaacatc aaaccagtta tcctggctgc tgacggctct gagccagatc
     5581 tttctcagca agccctgact gaaaaaggcg tgctgttcaa ctctggcgaa ttcaacggtc
     5641 ttgaccatga agcggccttc aacgccatcg ccgataaact gactgcgatg ggcgttggcg
     5701 agcgtaaagt gaactaccgc ctgcgcgact ggggtgtttc tcgtcagcgt tactggggcg
     5761 cgccgattcc gatggtgacg ctggaagacg gtaccgtaat gccgaccccg gacgaccagc
     5821 tgccggtgat cctgccggaa gatgtggtga tggacggcat taccagcccg attaaagcag
     5881 atccggagtg ggcaaaaact accgttaacg gtatgccagc gctgcgtgaa accgacactt
     5941 tcgacacctt tatggagtcc tcctggtact atgcgcgcta cacttgccca gagtacaaag
     6001 aaggtatgct ggattccgaa gcagctaact actggctgcc ggtggatatc tacattggtg
     6061 gtatcgaaca cgccattatg cacctgctct acttccgctt cttccacaaa ctgatgcgtg
     6121 atgcaggcat ggtgaactct gacgaaccag cgaaacagtt gctgtgtcag ggtatggtgc
     6181 tggcagatgc cttctactat gttggcgaaa acggcgaacg taactgggtt tccccggttg
     6241 atgctatcgt tgaacgtgac gagaaaggcc gtatcgtgaa agcgaaagat gcggcaggcc
     6301 atgaactggt ttataccggc atgagcaaaa tgtccaagtc gaagaacaac ggtatcgacc
     6361 cacaggtgat ggttgaacgt tacggcgcgg acaccgttcg tctgtttatg atgtttgctt
     6421 ctccggctga tatgactcta gaatggcagg aatccggtgt ggaaggggct aaccgctttc
     6481 tgaaacgtgt ctggaaactg gtttacgagc acacagcaaa aggtgatgtt gcggcactga
     6541 acgttgatgc gctgactgaa gatcagaaag cgctgcgtcg cgatgtgcat aaaacgatcg
     6601 ctaaagtgac cgatgatatc ggccgtcgtc agaccttcaa caccgcaatt gcggcgatta
     6661 tggagctgat gaacaaactg gcgaaagcac caaccgatgg cgagcaggat cgcgctctga
     6721 tgcaggaagc gctgctggcc gttgtccgta tgcttaaccc gttcaccccg cacatctgct
     6781 tcacgctgtg gcaggaactg aaaggcgaag gcgatatcga caacgcgccg tggccggttg
     6841 ctgacgaaaa agcgatggtg gaagactcca cgctggtcgt ggtgcaggtt aacggtaaag
     6901 tccgtgccaa aatcaccgtt ccggtggacg caacggaaga acaggttcgc gaacgtgctg
     6961 gccaggaaca tctggtagca aaatatcttg atggcgttac cgtgcgtaaa gtgatttacg
     7021 taccaggtaa actcctcaat ctggtcgttg gctaagcgcg ggaggaagcg tgcgatatct
     7081 ggcaacattg ttgttatctc tggcggtgtt aatcaccgcc gggtgtggct ggcatctgcg
     7141 tgataccacg caggttcctt ccactatgaa ggtcatgatc ctggactcag gcgatccgaa
     7201 cgggccatta agccgtgcgg tgcgtaacca gttacgtctg aatggtgtcg agttgcttga
     7261 taaagaaacc acgcgtaagg acgttccatc cctgcgtttg ggtaaagtga gcatcgcgaa
     7321 agataccgca tcggtattcc gtaacggtca aacagcagag tatcagatga tcatgacggt
     7381 gaatgcgacc gtgttgatcc ccggccgtga tatctacccg attagcgcca aagtcttccg
     7441 ttcgttcttt gataacccgc aaatggcgtt agcgaaagat aacgaacaag acatgatcgt
     7501 aaaagagatg tacgaccgtg ctgccgaaca gctgattcgt aagctgccaa gcatccgtgc
     7561 tgcggatatt cgttccgacg aagaacagac gtcgaccaca acggatactc cggcaacgcc
     7621 tgcacgcgtc tccaccacgc tgggtaactg atgattcggt tgtacccgga acaactccgc
     7681 gcgcagctca atgaagggct gcgcgcggcg tatcttttac ttggtaacga tcctctgtta
     7741 ttgcaggaaa gccaggacgc tgttcgtcag gtagctgcgg cacaaggatt cgaagaacac
     7801 cacacttttt ccattgatcc caacactgac tggaatgcga tcttttcgtt atgccaggct
     7861 atgagtctgt ttgccagtcg acaaacgcta ttgctgttgt taccagaaaa cggaccgaat
     7921 gcggcgatca atgaacaact tctcacactc accggacttc tgcatgacga cctgctgttg
     7981 atcgtccgcg gtaataaatt aagcaaagcg caagaaaatg ccgcctggtt tactgcgctt
     8041 gcgaatcgca gcgtgcaggt gacctgtcag acaccggagc aggctcagct tccccgctgg
     8101 gttgctgcgc gcgcaaaaca gctcaactta gaactggatg acgcggcaaa tcaggtgctc
     8161 tgctactgtt atgaaggtaa cctgctggcg ctggctcagg cactggagcg tttatcgctg
     8221 ctctggccag acggcaaatt gacattaccg cgcgttgaac aggcggtgaa tgatgccgcg
     8281 catttcaccc cttttcattg ggttgatgct ttgttgatgg gaaaaagtaa gcgcacattg
     8341 catattcttc agcaactgcg tctggaaggc agcgaaccgg ttattttgtt gcgcacatta
     8401 caacgtgaac tgttgttact ggttaacctg aaacgccagt ctgcccatac gccactgcgt
     8461 gcgttgtttg ataagcatcg ggtatggcag aaccgccggg gcatgatggg cgaggcgtta
     8521 aatcgcttaa gtcagacgca gttacgtcag gccgtgcaac tcctgacacg aacggaactc
     8581 accctcaaac aagattacgg tcagtcagtg tgggcagagc tggaagggtt atctcttctg
     8641 ttgtgccata aacccctggc ggacgtattt atcgacggtt gatatgaaat ctttacaggc
     8701 tctgtttggc ggcacctttg atccggtgca ctatggtcat ctaaaacccg tggaaacgct
     8761 ggcgaatttg attggtctga cgcgggtcac aatcatccct aataatgttc ctccgcatcg
     8821 tccccagccg gaagcgaaca gcgtgcagcg taaacacatg cttgaactgg cgattgccga
     8881 caagccatta tttactcttg atgaacgcga gctaaagcgc aatgccccct cttacactgc
     8941 gcaaacactg aaagagtggc ggcaggaaca aggaccggac gtgccgctgg cgtttattat
     9001 tggtcaggat tcactgctga cctttccgac ctggtacgaa tacgaaacga tactcgacaa
     9061 tgcacatttg atcgtctgtc ggcgtccagg ttacccactt gaaatggcgc aaccgcaata
     9121 ccagcaatgg ctggaagatc atttgacaca taaccaggaa gatcttcacc ttcagcctgc
     9181 cggtaaaatt tatctggctg aaacgccgtg gtttaacatc tcggcgacca tcatccgcga
     9241 acgtttgcaa aacggtgaat cgtgtgagga tttattgccg gaaccggtac tgacttacat
     9301 taaccaacaa ggcttgtatc gctgatacct gtcgttacgg agggcatgat gcgactgtgg
     9361 ttaattcgtc atggtgaaac gcaagcgaat atcgatggtc tttacagcgg tcatgcgccc
     9421 cccctgaccg cgcgcggtat tgagcaagcg caaaatctgc atacgctgct acatggtgtt
     9481 tcctttgatc tggttttatg cagtgaactg gaacgggcac agcataccgc gcgactggtt
     9541 ctcagtgacc gccagctccc cgtgcaaatc atccctgaac tcaacgaaat gttttttggc
     9601 gactgggaga tgcgacatca tcgcgacctc atgcaagaag atgccgaaaa ctatagcgcg
     9661 tggtgcaatg actggcagca tgcaatcccc acgaacggtg aaggatttca ggcattttcg
     9721 caacgtgtgg aacgctttat cgcaaggctt agtgaatttc agcactatca gaatatttta
     9781 gtcgtcagcc atcagggtgt actgagtctg ttaatcgccc gtttaattgg catgcctgcc
     9841 gaggctatgt ggcattttcg cgttgaccag gggtgttgga gcgccattga tatcaaccaa
     9901 aaattcgcga cgctacgcgt cctcaatagc cgtgccatcg gggtcgataa tgcatgactt
     9961 ttctgttttt ttacgggtaa gccgcaacga ccattgacag acccgggcag gctgatattc
    10021 tccgcagcca gacttttttc gccagacacg actttgtaga aattgtttta caaaaatggc
    10081 gatgcaatct gcggcgcggg gtgggatgat agcccacttt cgaaagccga ttcggcgaca
    10141 attgtcccga aatcgcctct ggttcaggta tactgacaga ccatttttat ctatttgatt
    10201 cacccagggg gaaaacttgc agggtaaagc actccaggat tttgttatcg acaaaattga
    10261 tgacctcaaa ggtcaggaca tcatcgcctt agacgttcaa ggcaaatcca gcatcaccga
    10321 ctgcatgatc atctgtacgg gtacgtccag ccgtcatgtt atgtccattg ctgaccacgt
    10381 tgtgcaggag tctcgcgcag cgggcctgtt accgctcggc gtagaaggtg aaaacagcgc
    10441 cgactggatt gtcgtggatt tgggcgatgt gattgtccat gtcatgcagg aagagagccg
    10501 tcgcctgtat gaactggaaa aactctggag ttaatgcgtg aagctgcaac ttgtcgccgt
    10561 gggaacgaaa atgccggact gggtacaaac cggttttacc gagtacctgc gtcgttttcc
    10621 gaaagatatg cccttcgagc tgattgaaat tccggccgga aaacgcggca agaatgcgga
    10681 catcaagcgc atactcgaca aagagggtga gcagatgttg gcggccgcag gcaaaaaccg
    10741 cattgtcacc ctcgatattc caggcaagcc ctgggatacg ccgcagttag ccgctgagct
    10801 ggaacgctgg aagctggatg gtcgcgacgt cagtctactg attggcgggc ctgaagggtt
    10861 gtcgcctgcc tgtaaagcgg cggcggagca gagctggtcg ctgtcggcgc ttaccctccc
    10921 ccatccgctg gttcgcgtgc tggtcgcaga gagtctgtac cgggcgtgga gcatcaccac
    10981 caaccatcct tatcaccgtg agtgataagg gagctttgag tagaaaacgc agcggatgaa
    11041 actacagaac tcttttcgcg actatacggc agagtccgcg ctgtttgtgc gccgggcgct
    11101 ggtcgccttt ttggggattt tgctgctgac cggcgtgctt atcgccaacc tgtataatct
    11161 gcaaattgtt cgctttaccg actaccagac ccgctctaat gaaaaccgca ttaagctggt
    11221 gcctatcgcg cccagccgcg gcattattta cgaccgtaat ggtatccctc tggccctcaa
    11281 ccgcactatc taccagatag aaatgatgcc agagaaagtc gataacgtgc agcaaacgct
    11341 ggacgctttg cgcagcgtgg ttgatctgac cgatgacgat attgctgcat tccgaaaaga
    11401 gcgcgcacgt tcacaccgtt tcacctctat tcccgtgaaa actaacctga ccgaagtaca
    11461 agtagctcgc tttgccgtca atcagtaccg ttttccgggg gttgaagtta aaggctataa
    11521 acgtcgttac tatccttacg gttcggcgtt gacccacgtc atcggctatg tgtcgaaaat
    11581 caacgataaa gacgtcgaac gcctgaataa tgacggcaaa ctggccaact atgcggcaac
    11641 gcatgatatc ggtaagctgg gcattgagcg ttactatgaa gacgtgctgc acggtcagac
    11701 cggttatgaa gaggttgaag ttaacaaccg tgggcgtgtt atccgccagt taaaagaagt
    11761 accaccgcaa gccggacacg atatttacct gacgctggat ctcaaactcc agcaatatat
    11821 tgaaacgctg ctggcgggta gccgcgcagc tgtggtagtc accgatccgc gtacaggtgg
    11881 ggtgctggcg ctggtttcca cgcctagtta tgacccaaac ttgtttgttg acggtatctc
    11941 cagcaaagat tattccgcct tgttgaacga cccgaataca ccgctggtga accgcgccac
    12001 acagggggtt tatcctcccg cgtctacagt taaaccctat gtggcggttt cggcattgag
    12061 cgccggggtg atcacgcgca atacgacgct gtttgaccca ggctggtggc aactgccagg
    12121 ttcggaaaaa cgttatcgtg actggaaaaa atggggccac gggcgtctga atgtcacaag
    12181 atcgctggaa gaatctgcgg ataccttctt ctatcaggtg gcctacgata tggggatcga
    12241 tcgcctctcc gaatggatgg gtaaattcgg ttatggtcat tacaccggta tcgacctggc
    12301 ggaagaacgt tccggcaaca tgcctacccg cgaatggaaa cagaaacgct ttaaaaaacc
    12361 gtggtatcag ggtgacacca ttccggttgg tatcggtcag ggttactgga cagcgacccc
    12421 aatccagatg agtaaggcac tgatgatcct gattaatgac ggtatcgtga aggttccgca
    12481 tttgctgatg agcaccgccg aagacggcaa acaggtgcca tgggtacaac cgcatgaacc
    12541 gcccgtcggc gatattcatt ccggttactg ggagctggcg aaagacggta tgtacggtgt
    12601 tgctaaccgc cctaacggta cgacgcataa atactttgcc agcgcaccgt acaaaattgc
    12661 ggcgaaatct ggtaccgctc aggtcttcgg tctgaaagcg aacgaaacct ataatgcgca
    12721 caaaattgcc gagcgtttac gtgaccacaa actgatgacc gcctttgcgc catacaacaa
    12781 tccgcaagtg gctgtcgcca tgattctgga gaacggcggc gcgggtccgg cggttggtac
    12841 gctgatgcgt cagatcctcg accacattat gctgggtgat aacaacaccg atctgcctgc
    12901 ggaaaatcca gcggttgccg cagcggagga ccattaatca tgacggataa tccgaataaa
    12961 aaaacattct gggataaagt ccatctcgat cccacaatgc tgctgatctt actggcattg
    13021 ctggtttaca gcgccctagt tatctggagc gccagcggtc aggatattgg catgatggag
    13081 cgtaaaatcg gccaaatcgc gatgggtctg gtcatcatgg tggtgatggc gcaaattcct
    13141 ccacgcgttt atgaaggctg ggccccctat ctctatatca tctgtattat tttgctggtg
    13201 gcggtagatg ctttcggtgc catctctaaa ggtgctcaac gctggctgga cctcggtatt
    13261 gttcgttttc agccgtcgga aattgccaaa atagccgtac cactgatggt tgcgcgcttt
    13321 atcaaccgcg acgtttgccc accgtcgttg aagaacacag gcatcgcgct ggtgctgata
    13381 tttatgccca cgctgctggt ggctgcacag cctgacctgg gaacatcaat cctcgttgcg
    13441 ctttccggtc tgtttgtact gttcctctct ggccttagct ggcgtctgat tggcgtcgca
    13501 gtagtgctgg tggcggcgtt cattcctatt ctgtggttct tcctgatgca tgattaccag
    13561 cgccagcgcg taatgatgct cctagacccg gaatcagacc cactcggcgc gggctatcac
    13621 attattcagt ctaaaattgc tattggctcc ggcggattac gcggcaaagg ctggctgcat
    13681 ggcactcagt cacaacttga atttctaccc gaacgccata ccgactttat cttcgcggta
    13741 ctggcggaag agctgggatt agtgggcatt ttgattctgc tcgctctcta cattctgctg
    13801 atcatgcgcg ggctgtggat agccgccaga gcgcaaacca cctttggtcg cgtcatggct
    13861 ggcggcttaa tgctgatatt attcgtttat gtcttcgtaa atattggtat ggtaagcggt
    13921 attctgccgg ttgtaggggt tccgctccca ctggtcagtt atggaggatc agcgctaatt
    13981 gtgctgatgg ctgggttcgg gattgtaatg tcaatccaca cccacaggaa aatgttgtcg
    14041 aaaagcgtgt aagaggtgcg caatgcgtaa gcagtggctc gggatctgca tcgcggcagg
    14101 aatgctcgcg gcatgtacaa gcgatgatgg tcagcaacag acggtaagtg taccgcagcc
    14161 tgcggtatgt aacggcccca tagttgaaat tagcggggcg gacccgcgtt tcgaaccact
    14221 gaacgcgacg gcaaatcagg attaccagcg cgacggtaaa agctacaaaa tcgtgcagga
    14281 tccgtctcga tttagccagg cgggactggc ggcaatctat gatgccgaac caggcagtaa
    14341 cctgacggcc tctggcgaag ctttcgatcc gacacagctg acggcggccc atccaacgct
    14401 tccgatcccc agctacgcca gaatcactaa cctggctaac gggcgaatga tcgtggtgcg
    14461 cattaatgat cgcggccctt acggcaacga ccgcgttatt tcgctttctc gcgcggcagc
    14521 tgaccgtctt aacacgtcaa acaacaccaa agttcgtatc gatccgatta ttgtcgccca
    14581 ggatggttcg ctttctggtc ctggtatggc ttgtaccaca gtcgccaaac agacttacgc
    14641 cctgcctgca cctcccgatt taagcggtgg cgcgggaaca agttcagtgt ctggcccgca
    14701 gggtgacatt cttccggtca gtaattcgac gctaaaaagc gaagatccga ccggcgcgcc
    14761 ggtaaccagt agcggtttcc tcggcgcacc aacgacctta gcgcctggtg tactggaagg
    14821 cagcgaaccg acgcctgcgc cacagcccgt tgttacagct ccgtcgacaa cgcctgcaac
    14881 ctcgcctgca atggtgacac cgcaagccgc ctcgcaaagc gccagcggca actttatggt
    14941 gcaagtcggg gccgttagcg atcaggctcg tgcgcaacag taccaacagc aactgggaca
    15001 gaagttcggc gtccccggtc gcgtaactca aaatggcgcg gtctggcgga tccagcttgg
    15061 ccccttcgcc agcaaagccg aagccagtac cttgcagcaa cgtttgcaaa ccgaagccca
    15121 attacagtca tttattacca ccgcgcagta gcgtaaagca ggcatctgat gtgtcaattt
    15181 ctgtaaatga cgttaacaaa gtcacgcgga aagtcagatg cctgccggta gtgcatttgc
    15241 tatagtaggg cacttttttt aattccatca cggatgtcgt agttcagacc atgaatacca
    15301 ttttttccgc tcgtatcatg aagcgcctgg cgctcaccac ggctctttgc acagccttta
    15361 tctctgctgc acatgccgat gacctgaata tcaaaactat gatcccgggt gtaccgcaga
    15421 tcgatgcgga gtcctacatc ctgattgact ataactccgg caaagtgctc gccgaacaga
    15481 acgcagatgt ccgccgcgat cctgccagcc tgaccaaaat gatgaccagt tacgttatcg
    15541 gccaagcaat gaaagccggt aaatttaaag aaactgattt agtcactatc ggcaacgacg
    15601 catgggccac cggtaacccg gtgtttaaag gttcttcgct gatgttcctc aaaccgggca
    15661 tgcaggttcc ggtttctcag ctgatccgcg gtattaacct gcaatcgggt aacgatgctt
    15721 gtgtcgccat ggctgatttt gccgctggta gccaggacgc ttttgttggc ttgatgaaca
    15781 gctacgttaa cgccctgggc ctgaaaaaca cccacttcca gacggtacat ggtctggatg
    15841 ctgatggtca gtacagctcc gcgcgcgata tggcgctgat cggccaggcg ttgatccgtg
    15901 acgtaccgaa tgaatactcg atctataaag aaaaagaatt tacgtttaac ggtattcgcc
    15961 agctgaaccg taacggcctg ttatgggata acagcctgaa tgtcgacggc atcaaaaccg
    16021 gacacactga caaagcaggt tacaaccttg ttgcttctgc gactgaaggc cagatgcgct
    16081 tgatctctgc ggtgatgggc ggacgtactt ttaaaggccg tgaagccgaa agtaaaaaac
    16141 tgctgacctg gggcttccgt ttcttcgaaa ccgttaaccc actgaaagta ggtaaagagt
    16201 tcgcctctga accggtttgg tttggtgatt ctgatcgcgc ttcgttaggg gttgataaag
    16261 acatgtacct gaccattccg cgtggtcgca tgaaagatct gaaagccagc tatgtgctga
    16321 acagcagtga attgcatgcg ccgctgcaaa agaatcaggt cgtcggaact atcaacttcc
    16381 agcttgatgg caaaacgatc gagcaacgcc cgctggttgt gttgcaagaa atcccggaag
    16441 gtaacttctt cggcaaaatc attgattaca ttaaattaat gttccatcac tggtttggtt
    16501 aaaaattaaa cacttgaaag tgtaatttcc gtccccatat actaagcatc agtaaaaaaa
    16561 ctcccgcctt ctggcgggag ttgctattta attacgttac gccggagctg acatgaaaac
    16621 caaacttaac gaactgcttg aattccctac tccttttact tacaaagtta tggggcaggc
    16681 gttacctgag ctggttgatc aggtggttga agtggtacag cgccatgcgc caggtgacta
    16741 caccccaacg gtaaaaccaa gcagcaaagg caactaccac tcggtatcta tcactatcaa
    16801 cgccactcat atcgagcagg ttgaaatact gtatgaagaa ctgggcaaaa tcgatattgt
    16861 ccgcatggtt ctgtaactcg cttctccgtt acccggctcc tggtcgggta actcccctct
    16921 cctcgctgtg atatactttt cccccacttt tactcattct ccacggagat gccgttttgt
    16981 atcaggataa aattcttgtc cgccagctcg gtcttcagcc ttacgagcca atctcccagg
    17041 ctatgcatga attcaccgat acccgcgatg atagtaccct tgatgaaatc tggctggtcg
    17101 agcactatcc ggtattcacc caaggtcagg caggaaaagc ggagcacatt ttaatgccgg
    17161 gtgatattcc ggtgatccag agcgatcgcg gtgggcaggt gacttatcac gggccgggac
    17221 aacaggtgat gtatgtgttg cttaacctga aacgccgtaa actcggtgtg cgtgaactgg
    17281 tgaccttgct tgagcaaaca gtggtgaata ccctggctga actgggtata gaagcgcatc
    17341 ctcgggctga cgcgcctggt gtctatgtcg gggaaaagaa aatttgctca ctgggtttac
    17401 gaattcgacg cggttgttca ttccacggtc tggcattaaa cgtcaatatg gatctttcac
    17461 catttttacg tattaatcct tgtgggtatg ccggaatgga aatggctaaa atatcacaat
    17521 ggaaacccga agcgacgact aataatattg ctccacgttt actggaaaat attttagcgc
    17581 tactaaacaa tccggacttc gaatatatta ccgcttaatt ccatacatca atggcccaat
    17641 ttacactggg tcattactca taatacctgc taatgcttta catctccttc gcgtttgtat
    17701 attttcttta tcacttgcag aaatgaggtg ccacgccttg ctccgtaact caccccattg
    17761 attaacctat tgaaatagtt gattatggtg agcaaaagcg ttccgttcca attagacata
    17821 agattattgt aattttttaa atattatttt tccatgaata aaattggagt aagtgcgtgg
    17881 atagtaataa tcaaattgaa ccctgtttaa gcagaaagtc gtcagaaggt aaaccacaaa
    17941 tatttacaac cttacgaaat attgctctta accttctgac tatttttgaa gctgtatatg
    18001 tacataaagg gatcgttaat gcagcgaaag tgcttaatct gcccccctcg gcaatcagtc
    18061 agtctattca gaaactgcgc gttatattcc ctgacccatt gtttattcgc aaaggccaag
    18121 gtgtcactcc taccgcattt gcgatgcatc tacatgagta tatcagtcag ggccttgagt
    18181 ccattcttgg cgcgctggat atcgaaggaa gctatgataa gcaacgaacg ataactattg
    18241 cgaccactcc ctcggtcgga gccctggtcc ttcctgtcat ctatcgggcg attaaaactc
    18301 actatccgca gcttttactg cgcaacccgc ccatcagcga cgcggaaaac caactcagtc
    18361 agtttcaaac cgatctcatc atcgataaca tgttttgcac caatcgtacg gtgcaacatc
    18421 atgttctgtt caccgacaat atggtgttaa tttgccgcga gggaaatcca ctactctctt
    18481 tagaagatga cagagagact atcgacaacg ctgcgcatgt acttctgtta ccggaagagc
    18541 aaaatttcag cggtctgcgg cagagagttc aagaaatgtt tccggaccgg caaatcaatt
    18601 tcaccagcta caacattttg acaatcgctg cactggttgc caacagtgac atgttagcga
    18661 ttattcccag ccgtttttat aacctgttta gccgctgctg gccgctggaa aaactgcctt
    18721 ttccgtcctt aaataaggag caaatagatt tctctatcca ctacaacaaa ttcagcctgc
    18781 gtgatcccat cctgcacgga gtaatagatg ttatccgtaa tgcattttga aaaagtattg
    18841 ttacagccag atcacagcgc agaacgataa aatggcacaa tccaacaaca attttacatt
    18901 ttcgcgaccg ctttggctgc tttcaggtcc gtttcaatga tatactgcca gtcgttaatt
    18961 caaaaatagt tgataattac aacaatctat tgaattgaaa cgctttcctt cgtaattcgc
    19021 aactggaaca cgcacgctat gagtaaaccc attgtgatgg aacgcggtgt taaataccgc
    19081 gatgccgata agatggccct tatcccggtt aaaaacgtgg caatagagcg cgaagccctg
    19141 ctgcgcaagc cggaatggat gaaaatcaag cttccagcgg actctacacg tatccagggc
    19201 atcaaagccg caatgcgcaa aaatggcctg cattctgtct gcgaggaagc ctcctgccct
    19261 aacctggcgg aatgcttcaa ccacggcaca gcaacgttta tgatcctcgg cgctatttgt
    19321 acccgccgtt gtccgttctg tgatgttgcc cacggtcgcc cggtagctcc tgatgccaat
    19381 gaaccagtga aactggcgca gaccattgcc gatatggcgc tgcgttatgt ggttatcacc
    19441 tccgttgacc gtgatgacct gcgcgatggc ggtgcccagc actttgcgga ttgcattact
    19501 gccattcggg aaaaaagccc gcaaatcaaa attgaaactc tggtgccgga tttccgtggt
    19561 cgtatggatc gtgctctgga tattctgact gcaacgccac cagatgtgtt caaccataac
    19621 ctggaaaacg taccgcgtat ttaccgtcag gtacggcctg gtgcagatta caactggtcg
    19681 ctgaagctgc tggaacgctt taaagaagcg catccggaaa tcccgaccaa gtctggtcta
    19741 atggtgggac tgggtgaaac caatgaagaa attattgagg taatgcgcga cctgcgccgt
    19801 catggtgtga cgatgttaac gctggggcaa tatttgcagc caagccgcca tcacctgccg
    19861 gttcaacgtt acgttagccc ggatgagttc gacgaaatga aagccgaagc gctggcgatg
    19921 ggctttaccc atgctgcatg cggtccgttt gtccgttctt cttaccacgc cgatttgcag
    19981 gcgaaaggga tggaagttaa gtaatcctgt aataattact tacatctgtc tgataaaaaa
    20041 cccgcttttt gaagcggttt ttttgtatcg aacaagatat tgagggagcg tcctgctcgc
    20101 cacgtcactc tttatgagag agcttttcag cctgaagatc aacgtctgcg ccttttttcg
    20161 ccgcagcatc atcatcattc atcgccttct tgaacccttt aatggccgct ccaaggtctc
    20221 cgcccagcgt acgtaacttc ttagtcccaa acagcagaac gaccagcgcc gcaactacta
    20281 gcagtttggt aatactaatc tcacccatag ataccttctt gacatataac cggcaacaga
    20341 acgcgcctta aaaaccagag aatattaacg gtcattcgac gcgcgcacac aatagcaatc
    20401 tgtaacaagg tgaatcaagc gccgagtaaa aaaacatcat aataattgcg gcggcgcaaa
    20461 gcgacggttg tttaagacgg gcagttgcgc gcgcacctga cgcacacgtt cgggcgtcac
    20521 ttccgccata atgagtgcag gcatttctga cgctgccgca atggtgacgc caaagggatc
    20581 tataatccgg ctttgcccga tatttttgtt cccgcactcg cccgccgcca ccatataaca
    20641 ggtggtatcc agcgcacgag cggcaagcaa cgttgaccag tgatgctctt tgagcggccc
    20701 gcgaacccag gcggcaggaa gtaccaggat ttcagctccc tgtaatgcct gtgccagcgc
    20761 cagctctgga aagcgtaagt cataacaggt catcagaccg accttcatcc cttccacctc
    20821 cagtaacgga gcgatttcat taccagcatc aacacggcgt gattcctgaa tggcaaatgc
    20881 atcatagaga tgcagtttgg cataacgggc gacgatgttt cctgcctgaa gtgccaccag
    20941 catattccat gcgcgccccg gcgttgaagg aacatgaatc gtcagaattg tcgtcatcat
    21001 gttacgttta ctttctcgcc gtaaacgtcc gaggaattcg ccttccagca gctgtgctga
    21061 tttaaccgat agatctgcat catgatcatc gcgtgccagc aatgcttccg gcaggacaaa
    21121 cagcgatacg gcgttttccg ccgcctgcgc catcaacgag gcacaaatct cagcgttctt
    21181 ttcccacaca gatgtaacag caaactgtcc tgcagcaacc agcattgcca cctcctgtca
    21241 gagtcgtaaa attcgccaac gacgattatt cggcgttaca cttatcactc atacaaatca
    21301 aatagcagga ttttgcagtg ttacaacttc ttttagcagt ttttattggc ggtggtacgg
    21361 gaagcgtggc gagatggctg ttaagtatgc gatttaaccc actgcatcag gcgattccgt
    21421 tggggacgct gacagcaaac ctgattgggg cattcatcat aggaatggga ttcgcatggt
    21481 tcagcaggat gacgaacatt gatccagtgt ggaaagtatt aatcaccacc ggattttgtg
    21541 gcggtctaac aaccttctca acattttcgg cagaagtggt gtttttgtta caagagggtc
    21601 gctttggctg ggcattactg aacgttttcg tcaaccttct ggggtctttt gccatgacag
    21661 cactggcttt ctggctgttt tcggcctcaa ccgcacacta aagacccaaa aaaaacccgc
    21721 tgattaagcg ggttttgaat tcttgctgac gtatcttaca gagcgattac gtttgcagca
    21781 gaagggcctt tggcaccgtt agtgatttcg aactctacgc gctgaccttc agcaagagtt
    21841 ttaaaaccat tagtctggat tgcagagaag tgtacgaaca cgtctttgct gccgtcttcc
    21901 ggagtaatga aaccgaatcc tttggactca ttaaaccact taacgttacc tttaatctta
    21961 gacatcaaaa ttacctttac atgaaaaata gacacaaatg ctgcgtcggt taccagtaca
    22021 ccaattgtgg tacgctttgt ccagtgcaac tttactaaaa agtgataaaa gtcgcgttat
    22081 ttttgatgtt acttatttta gctattgatt ttaaagaagt tactaaaact tcatttgtct
    22141 caaaactgaa agcgcatcca ggcaaagtac acattgccat tgttgtaggt acccggaatg
    22201 taggtcatct gaaaagtcac tgggccataa cccacggagg ccaatggcag tagaaccggg
    22261 agagggatgt aattccagtt atcgcgtgcc gttacgccag cggtgaatcc cagacctaaa
    22321 tgaaaatttt catccgccag cggtcgccag gtactttccc atccgtatcc ggcaatcggt
    22381 tcccatttgt tccacgagtc cttaaatgcc atggcataca ggccatgcca gtttcctttt
    22441 tcatcccaac gcgacaggcc aaaaccgcca ccccacggtc gctcgttata gcgatcggtt
    22501 ttttctttgt cgtaagcgaa acgtgcatgc caggtgatgg caggaatata taaatcataa
    22561 tgttcaggct gttgccaggt tggtgcaata ttttctctaa acgttgtcat ccactcatct
    22621 gcgttagcaa aaactttacc aacgctgatt aactgaataa aaacaaagga aaagatagcg
    22681 acatatttac tcacgttcat ttgtgaccat aaaacattta tcaaaaatct actactagca
    22741 tagcaaagct acaattaaca taaccttaat agacccaaca taaagaataa tctgaatagc
    22801 tgctctattt atttccgctt gttcaagtta aatatttccg cgcatcgatt aaagatgatg
    22861 cgtcgttttc tgcgatggga atagtcaaaa aagaaaaacc aagtcttttt tgatgacaaa
    22921 atgcaatcaa ggaaaaatta ttttattttt taacgagata caacaatcac cttaacaaag
    22981 tatataatat atatcgtcaa cctatgctca tattagatta ttttttgagc gcacagccgc
    23041 agctaacacc attgcaatta acaaatttgc atcaatccac catcaatttg cacacattat
    23101 tatgtgataa ttgccaaccg ctaaatatgc gttttgttat ctatgtataa aaacagtaac
    23161 ttcaatgtct taatggcagt ttttcttgat tttaatcagc attcatcgcc aatttattgg
    23221 gcatattttt tccttaagct ttaggaattt tttattattt actttggggc ctggagacag
    23281 gaaaaattat gctgacattc attgaactcc ttattggggt tgtggttatt gtgggtgtag
    23341 ctcgctacat cattaaaggg tattccgcca ctggtgtgtt atttgtcggt ggcctgttat
    23401 tgctgattat cagtgccatt atggggcaca aagtgttacc gtccagccag gcttcaacag
    23461 gctacagcgc cacggatatc gttgaatacg ttaaaatatt actaatgagc cgcggcggcg
    23521 acctcggcat gatgattatg atgctgtgtg gatttgccgc ttacatgacc catatcggcg
    23581 cgaatgatat ggtggtcaag ctggcgtcaa aaccattgca gtatattaac tccccttacc
    23641 tgctgatgat tgccgcctat tttgtcgcct gtctgatgtc tctggccgtc tcttccgcaa
    23701 ccggtctggg tgttttgctg atggcaaccc tatttccggt gatggtaaac gttggtatca
    23761 gtcgtggcgc agctgctgcc atttgtgcct ccccggcggc gattattctc gcaccgactt
    23821 caggggatgt ggtgctggcg gcgcaagctt ccgaaatgtc gctgattgac ttcgccttca
    23881 aaacgacgct gcctatctca attgctgcaa ttatcggcat ggcgatcgcc cacttcttct
    23941 ggcaacgtta tctggataaa aaagagcaca tctctcatga aatgttagat gtcagtgaaa
    24001 tcaccaccac tgctcctgcg ttttatgcca ttttgccgtt cacgccgatc atcggtgtac
    24061 tgatttttga cggtaaatgg ggtccgcaat tacacatcat cactattctg gtgatttgta
    24121 tgctgattgc ctccattctg gagttcctcc gcagctttaa tacccagaaa gttttctctg
    24181 gtctggaagt ggcttatcgc gggatggcag atgcgtttgc taacgtggtg atgctgctgg
    24241 ttgccgctgg ggtattcgct caggggctta gcaccatcgg ctttattcaa agtctgattt
    24301 ctatcgctac ctcgtttggt tcggcgagta tcatcctgat gctggtattg gtgattctga
    24361 caatgctggc ggcagtcacg accggttcag gcaatgcgcc gttttatgcg tttgttgaga
    24421 tgatcccgaa actggcgcac tcttccggca ttaacccggc gtatttgact atcccgatgc
    24481 tgcaggcgtc aaaccttggc cgtacccttt cgcccgtttc tggcgtagtc gttgcggttg
    24541 ccgggatggc gaagatctcg ccgtttgaag tcgtaaaacg cacctcggta ccggtgcttg
    24601 ttggtttggt gattgttatc gttgctacag agctgatggt gccaggaaca gcagcagcgg
    24661 tcacaggcaa gtaaatagta atgccggagg caagtttcct ccggctttat catgtcaccc
    24721 actgtggtat atgcgttgtg gtctgccaac tttgccgtga acaatttcag caataatcag
    24781 atggcggctg gcgcaatatt caagataacg tctggcagac gtgcggctga tggtcagtgc
    24841 ctgcgccacc gtttctgccg tatgttgcac accaggctct ttaaacagtt ttcgcaccgc
    24901 gtttagcgtc aagggatcaa tgccggtcgg cagttcgtct ttaggttcac cgcgagcata
    24961 ggcattaaac atctcatcaa tttgcttctg gctggcgcta tcaatacttt ccagcatatg
    25021 tttacgctgg cggaaacggg ttagcgtttg ccccagccgt tcataggcaa tgggcttaat
    25081 gagataatca aatacaccac aacgtacggc ttcagacacc gtttccatat cgctggctgc
    25141 agtggtaaac accacgtcgc cgggataatg cgcctgcacc agttcatgca ataaattaat
    25201 ccctctgccg tcaggaagat agttatcgag caagattagc cccggcttaa aacgctcgat
    25261 catcattcgg gcctgcgcca ggtttcccgc cagtaatatc tgactgaatc cgggaatgtg
    25321 acgaatatat tccgcatgca tctctgccag cggcgtttcg tcctcaacga tcaatagggt
    25381 taatggagct gtcattaggt ttcactttcg gaatatagat tgaaaataag gtaccgcagg
    25441 gatcattatc ttcgagagtg ataacaccac cgcagcgcgt tacgtagctg gcaatcaagt
    25501 acaacccaat gccatgttca ccgggctcgt cagcacgcgt actgaccccc tgctcaaata
    25561 ttttgtctcg tagagactct ggaacgccgc agccctgatc ggcgacttca atcaccacat
    25621 catcgccttc atcgctgagg aataattcaa cgatcttgtt tccttcatcg ctacgcaggc
    25681 tggcttcgaa ggcgttatca agtaaattgc ccgcaatggc tgcaaactcg gtgctatcca
    25741 gtcctggcgg cagttgcgaa agctggctac cggggacaat gatcattttt agccccagtt
    25801 cccgggcgcg ctgcacttta ccaaaaagca gccccgccac ctagcgatcg gcaaacgcct
    25861 cgcgcaggct gtcaataagc tgttgctggg cctgagactc cccctgcacc atcgccagca
    25921 cgcgatcata ctctttcatc tgcaacagac cattgagcgt cgacatccaa ttgagatgct
    25981 cgtgtcgcaa tgtacgaagg ctctcaacgt attgttttat ttgcgtcagt tgcgcattga
    26041 gggtggaaat ttcgtcttta ctgcgaaagc tgataatggc ccccagcaaa tcatcacctg
    26101 aacgaatagc ttcccgattg gcaataacgc tcagaccgtt aaagttagcc accatatcct
    26161 gacgtttttc atcaatctgt tcggtaaaga aatcggcggg cctgaccact tcagcaatgg
    26221 gtttacccaa ccattgccgt ccgggggaac tcagccccag catctttctt gcgttacgat
    26281 tgatggcggt aatgtaaccg tgcggatcca ccgcaatcag cccttcataa accgaactaa
    26341 acagcgcctc ttgctgacgg accacgcgtg cgatttgctt tggctccatg cccatcatct
    26401 gccgacggat atgcgcggcc aggaaccacg acagcaacat cagaatccct aacagcacga
    26461 caaacacacc tgccatcggt aataaaaact cagcccgcca gctatcgatt ttactcacca
    26521 ggtagccaat cgacaccacg ccgatgactt ttccatcgtc atcaaagatt ggcgttttgg
    26581 cgcgcatcgc catccccatt gaccctttcc cggtaatgaa gtagctctcc cctttctcca
    26641 gcgcgccctg tttggtgaac tgcataggat aaccaatttt ctccggatta ggatggtaaa
    26701 ggcggatcga gtgccggtcg ccatcaccac ataatcaaaa tcggtatctc tttgtaattt
    26761 gttagcgatg gtcgccagcc gtttgtagtc acgcgttttc accgcagaga tgacactgtc
    26821 attggaggca ataattttcg cctgattcat tgccatgtcg cgtacatgaa gcgttaaata
    26881 gtcctcaaaa ctggccgtaa aatattgcgc caaggctgca atgacaaata ttgagaacac
    26941 cagaatcagc aaaaagatac gcagcggaaa tgccagtctt tggaaaaatg caaactgttt
    27001 attctcgtta agctgcaaca ttgttttcct tgcgggttat cccgagcctc tttgacagat
    27061 gtaattaaaa tgttgaatca aaatacatag atattaatta tgatcgtggc aaattttatt
    27121 cattaaatca attaaatcac ttaaacctgc aggtcatttc gtaattacat taattaaaaa
    27181 acttaaacca ttaaataaat aaaaatttca cgccgaaata ctccttagga tgtatagcga
    27241 aaggagaaaa agatatacct cgatcacccc cctttctccc aagtgaaaat aaaaggttat
    27301 cagtttgcaa cattgaacaa cattcgttgc aaatcgataa caacatgcac cttcaggata
    27361 ctatttatta tgttcggcaa tgatattttc acccgcgtaa aacgttcaga aaataaaaaa
    27421 atggcggaaa tcgcccaatt cctgcatgaa aatgatttga gcgttgacac cacagtcgaa
    27481 gtatttatta ccgtaacccg cgatgaaaag cttatcgcgt gcggtggaat tgccggaaat
    27541 attattaaat gcgttgctat cagtgaatcc gttcgcggtg aaggactggc gctgacatta
    27601 gccactgaac tgataaacct cgcctatgag cggcacagca cgcatctgtt tatttatacc
    27661 aaaaccgaat acgaggcgct gttccgccag tgcggttttt ccacgctgac cagcgtaccc
    27721 ggcgtgatgg tgctgatgga aaacagcgcc acgcgactga aacgctatgc cgaatcgctg
    27781 aaaaaatttc gtcatccagg gaacaagatt ggctgcattg tgatgaacgc caatcccttt
    27841 acgaatggtc accgttatct gattcaacaa gctgcggcac agtgcgactg gttgcatctg
    27901 tttttagtca aagaagattc ttcacgcttc ccctatgaag accggctgga tctggtgtta
    27961 aaaggcaccg ccgatattcc acgcctgact gtgcatcgcg gctccgaata catcatctcc
    28021 cgcgctacgt tcccttgcta cttcattaaa gaacagagcg tcattaacca ttgttacacc
    28081 gaaattgatc tgaagatttt ccgtcagtac ctcgctcccg cactgggtgt aactcaccgc
    28141 tttgtcggta ctgaaccctt ttgtcgcgtt accgcccagt acaaccagga tatgcgctac
    28201 tggctgaaaa cgccgactat ctccgcaccg cccatcgaac tggttgaaat tgagcggctg
    28261 cgttaccagg agatgccgat atccgcttcc cgggtacgtc aactgctggc gaaaaacgat
    28321 ctcacggcta tcgcgccgct ggtccctgca gtcacgctgc attatttgca gaacctgctt
    28381 gagcactccc gccaggacgc ggcagctcgt caaaagaccc ccgcatgaga aacaggtgaa
    28441 aaatgaaaat aaaccagccc gccgttgcag gcacccttga gtctggggat gtgatgatac
    28501 gcatcgcccc actcgatacg caggatatcg acctgcaaat caacagcagc gttgagaaac
    28561 agtttggcga tgcaattcgc accaccattc tggacgttct cgcccgctac aacgtgcgcg
    28621 gcgtacagct gaatgtcgat gacaaaggca cactggactg cattttacgt gcacgactgg
    28681 aagccctgct ggcacgcgcc agcggtatcc cgactctgcc atgggaggat tgccaatgat
    28741 ttccgcttcg ctgcaacaac gtaaaactcg cacccgccgc agcatgttgt ttgtgcctgg
    28801 tgccaatgcc gcgatggtca gcaactcctt catctacccg gctgatgccc tgatgtttga
    28861 cctcgaagac tccgtggcat tacgtgaaaa agacaccgcc cgccgcatgg tttatcacgc
    28921 gctgcaacat ccgctgtatc gcgatattga aaccattgtg cgtgtcaacg cgctggattc
    28981 cgaatggggt gttaacgacc tggaagccgt cgttcgcggt ggtgcggacg ttgtgcgtct
    29041 gccgaaaacc gataccgctc aggatgttct ggatattgaa aaagagatcc tgcgtatcga
    29101 aaaagcctgt ggtcgtgaac ccggcagcac cggcctgctg gcggcgattg aatctccgct
    29161 gggcattacc cgcgcagtgg aaatcgctca cgcttccgag cgtttgatcg gtatcgccct
    29221 cggtgcagaa gactatgtgc gcaacctgcg tacagaacgc tccccggaag gaactgaact
    29281 gctgttcgca cgctgttcca ttttgcaggc cgcgcgctct gcgggtattc aggcgttcga
    29341 taccgtctat tccgacgcta acaacgaagc cggattcctg caagaagccg cccacatcaa
    29401 acagctgggc tttgacggca aatcgctgat caacccgcgt cagattgatc tgctgcacaa
    29461 cctctacgca ccgacccaga aagaagtgga tcacgcccgc cgcgtcgtag aagccgctga
    29521 agccgccgct cgcgaaggcc tcggcgtggt ttccctgaac ggcaagatgg tggacggtcc
    29581 ggttatcgat cgcgcccgtc tggtgctctc ccgtgcagaa ctttccggca tccgcgaaga
    29641 ataaggcaat caaaatgacg cagaaaattg aacaatctca acggcaagaa cgggtagcgg
    29701 cctggaatcg tcgcgctgaa tgcgatcttg ccgctttcca gaactcacca aagcaaacct
    29761 accaggctga aaaagcgcgc gatcgcaaac tgtgtgccaa cctggaagaa gcgattcatc
    29821 gctctggttt acaggacggc atgacggttt ccttccatca cgctttccgt ggcggtgacc
    29881 tgaccgtcaa tatggtgatg gacgtcatcg cgaagatggg ctttaaaaac ctgactctgg
    29941 cctccagctc cctgagtgat tgccatgcgc cgctggtaga acacattcgc cagggcgtgg
    30001 ttacccgcat ttatacctct ggcctgcgcg gtccactggc ggaagaaatc tcccgtggtc
    30061 tgctggcgga accggtgcaa atccactctc acggcggacg tgtgcatctg gtacagagcg
    30121 gcgaactgaa tatcgacgtg gctttcctcg gcgtcccatc ctgtgatgaa ttcggtaatg
    30181 ccaacggtta caccggtaaa gcctgctgtg gctccctcgg ctatgcaatg gttgatgccg
    30241 acaacgcaaa acaggtcgtg atgcttaccg aagaactgct gccttatccg cataatccgg
    30301 caagcattga gcaagagcag gttgatttga tcgtcaaagt tgatcgcgtt ggcgatgctg
    30361 caaaaatcgg cgctggtgcg acccgtatga ccactaaccc gcgcgaactg cttattgccc
    30421 gtagcgctgc ggatgtgatt gtcaactctg gctacttcaa agaaggtttc tccatgcaaa
    30481 ccggcaccgg cggcgcttcg ctggcggtaa cccgtttcct ggaagacaaa atgcgtagcc
    30541 gcgatattcg cgccgacttc gccctcggcg gtattactgc aacgatggtt gatctgcacg
    30601 aaaaaggtct gatccgcaaa ctgctggatg tgcagagctt tgacagccat gctgcgcaat
    30661 cgctggcccg taaccccaat cacatcgaaa tcagcgccaa ccagtacgct aactggggtt
    30721 cgaaaggcgc atcggttgat cgtctcgacg tggtggtact gagcgcgctg gaaattgaca
    30781 cccagttcaa cgttaacgtg ctgaccggct ctgacggcgt actgcgtggt gcttccggtg
    30841 gtcactgcga taccgcgatt gcctctgcgc tttccatcat cgtcgcgccg ctggtacgcg
    30901 gtcgtattcc gactctggtg gataacgtgc tgacctgcat caccccgggc tccagtgtcg
    30961 atattctggt cacagaccac ggtatcgcag ttaacccgac acgtccggag ctggcagaac
    31021 gtctgcagga agcgggcatt aaagtggttt ccattgagtg gctgcgtgaa cgtgcgcgtc
    31081 tgctgaccgg tgaaccacag ccgattgaat tcaccgaccg cgtcgttgcc gttgtgcgtt
    31141 accgcgatgg ctcggtgatc gatgttgtac atcaggtgaa ggaataagcc atgcacctgc
    31201 ttcctgaact cgccagccac catgcggtgt caattcccga gctgctcgtc agccgggatg
    31261 aaaggcaagc acggcaacac gtctggctca agcgccatcc tgttccactg gtctccttta
    31321 ccgtggttgc gcctaggccg attaaagaca gtgaggtcac acgccgaatt tttaatcatg
    31381 gcgtgacagc cttgcgtgcc ttagccgcaa aacagggctg gcaaattcag gagcaggctg
    31441 cactggtttc cgccagcggg ccggagggta tgttgagcat tgccgccccg gctcgcgacc
    31501 tcaagctcgc caccattgag cttgaacata gtcatcctct cgggcggtta tgggatatcg
    31561 atgtcctgac gcccgaaggc gaaattctct cccgccgcga ctattcactg ccgcctcgcc
    31621 gctgcctgtt gtgcgagcaa agcgcagccg tctgcgcgcg tggaaaaccc atcaactgac
    31681 cgatttactc aaccgcatgg aggcactgct gaacgatgtc gatgcctgca acgtcaacta
    31741 aaaccacaaa gcttgcgacg tcattaatcg atgagtacgc cctgctgggc tggcacgcca
    31801 tgctgactga agtcaatctg tcaccgaaac caggtctcgt ggatcgcatt aactgcggtg
    31861 cgcataaaga tatggcgctg gaagatttcc accgcagcgc gctggcgatt cagggctggc
    31921 taccccgttt cattgaattt ggtgcctgta gtgcggaaat ggcaccagaa gcggtactcc
    31981 acggattacg cccaattggt atggcttgcg aaggtgatat gttccgcgcc accgcgggcg
    32041 taaacacgca taagggcagc attttttctt tagggctact gtgtgcggca attggccgtt
    32101 tgcttcagct caaccagtcg ctaacgccaa caaccgtttg ttctacggcg gcaagtttct
    32161 gccgtggcct gaccgatcgc gaactgcgta ccaataattc acaactgacg gcaggtcaac
    32221 ggttgtacca gcagcttgcc cttaccggcg cacgcggtga agccgaagcg ggttatccac
    32281 tggtgatcaa ccacgccttg ccgcattacc tcactctgct ggatcagggg ttagatcctg
    32341 aactggcatt gctcgatacc ttgctcctac tgatggcgat caacggcgat accaacgttg
    32401 cttcgcgcgg tggcgagggg gcctgcgctg gctacagcgc gaggcgcaaa cattattgca
    32461 aaaagggggc attcgaaccc ccgccgatct cgattatctc cggcagttcg acagggagtg
    32521 tatcgaacga aatctcagtc taggcggcag tgccgacctg ctgatcctta cctggttttt
    32581 agcacagatt taattattta agcacttgat aaatttggaa atattaattt tcggagaacc
    32641 cgtatgtctt tagcaaaaga taatatatgg aaactattgg ccccactggt ggtgatgggt
    32701 gtcatgtttc ttatccctgt ccccaacggt atgccaccac aggcatggca ttacttcgct
    32761 gtgtttgtgg caatgattgt cggcatgatc ctcgagccaa ttccggcaac agcgatcagt
    32821 tttattgcgg ttactatttg cgttattggc agtaattacc tgctctttga tgccaaagaa
    32881 ttagctgacc cagcgtttaa tgcgcaaaaa caggcgctga aatggggtct ggctggtttt
    32941 tccagcacca cggtatggct ggtatttggc gcatttattt ttgcattagg gtatgaagtt
    33001 tccgggttag gtcgtcgcat tgcccttttc ctggtgaaat tcatgggcaa acgcacgctg
    33061 acgttgggtt atgcgattgt cattatcgac attctgctgg cgccgtttac accgtccaac
    33121 accgcgcgta ccgggggtac ggtttttccg gtcattaaaa acctgccgcc gttgtttaaa
    33181 tcattcccca acgatccgtc cgcgcgtcgt attggcggct atttgatgtg gatgatggtc
    33241 attagtacca gtctgagttc gtccatgttt gtcaccggtg cggcaccaaa cgtgctgggt
    33301 ctggagttcg tcagcaaaat tgccggtatc cagattagct ggttgcagtg gttcctctgc
    33361 ttcctgccgg ttggggttat cttgcttatc attgcgccgt ggctttccta cgtgctgtac
    33421 aaaccggaaa tcacacacag tgaagaagtg gcaacctggg cgggtgatga actgaaaacc
    33481 atgggtgcgc tgacacgcag agagtggaca ctgattggcc ttgtattgct cagcttaggt
    33541 ttgtgggttt ttggcagtga agtcattaat gctactgcag ttggtctgct ggcagtttcg
    33601 ctaatgctgg ccctgcacgt tgtgccttgg aaagatatta cccgctataa cagcgcatgg
    33661 aacacgctgg tcaacctggc aactctggtt gtgatggcta acggcctgac tcgttctggc
    33721 tttattgact ggttcgccgg taccatgagt acgcacctgg aaggattctc accaaacgca
    33781 acggtgattg tactggttct ggtgttctac tttgcacact acctgtttgc cagcctgtct
    33841 gcgcacaccg caaccatgct gccggttatt ctggccgtcg gtaaaggtat tccgggcgta
    33901 ccaatggaac aactgtgtat cctgctggtg ctgtctatcg gtatcatggg ctgtctgacg
    33961 ccctatgcaa ccggtcctgg ggtgattatt tacggctgtg gctatgtgaa atcaaaagat
    34021 tactggcgtc ttggcgcaat cttcggggtg atttatatct ctatgttgct gttggttggc
    34081 tggccgattc tcgccatgtg gaactaatac gttcgccaaa cgctgaataa atcatcacgc
    34141 ccgccaggta agcccacctg gcgggcgtga tgatttaata gatagtattc agtgtatgta
    34201 tgagttccac acccattatg aaagcattct ggcgtaacgc cgcgttgctc gcggtttctc
    34261 tgcttccctt ctcttctgcc aacgccgtag cgttgcaggc aaaacagtat ggcgattttg
    34321 atcgctatgt cctggccctc tcctggcaaa ccggattttg ccagagtcaa cacgatcgaa
    34381 atcgtaacga acgagatgaa tgtcgcctgc aaaccgaaac gaccaacaaa gctgattttc
    34441 tgaccgtaca tggtctgtgg ccaggattgc ctaaatcggt tgctgcccgt ggtgttgatg
    34501 aacgccgctg gatgcgcttc ggttgcgcta ctcgcccaat cccgaatcta ccagaagcgc
    34561 acgccagccg gatgtgttca tcgccggaaa ccgggttatc actggaaacg gccgctaaac
    34621 taagtgaagt catgccagga gctggcggac gttcctgcct ggaacgctac gaatatgcca
    34681 aacacggtgc ctgctttggt tttgatccgg acgcatactt cggtacgatg gtacgcctga
    34741 atcaagaaat taaagagagc gaagccggaa aattccttgc ggataattac ggtaaaacag
    34801 tgagccgccg tgactttgac gccgcctttg ccaaaagctg gggaaaagag aacgtgaaag
    34861 cagttaagct aacgtgccag ggtaaccctg cgtatttgac tgaaattcag atctcgatca
    34921 aagctgacgc catcaacgct ccgctttctg caaactcatt cttgccacaa ccacacccaa
    34981 gcaactgtgg caaaaccttt gtgattgata aagtgggtta ttaatcatct gatttacaaa
    35041 acacgctaat tcgtcttcca taaac
//
LOCUS       DAJTMC010000024        34543 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.024, whole genome shotgun sequence.
ACCESSION   DAJTMC010000024 DAJTMC010000000
VERSION     DAJTMC010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34543
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.024"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            112..718
                     /locus_tag="OO130_001026"
                     /pseudo
     CDS             112..718
                     /locus_tag="OO130_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544752.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            821..1323
                     /locus_tag="OO130_001027"
                     /pseudo
     CDS             821..1323
                     /locus_tag="OO130_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
     gene            complement(1390..2847)
                     /gene="nuoN"
                     /locus_tag="OO130_001028"
     CDS             complement(1390..2847)
                     /gene="nuoN"
                     /locus_tag="OO130_001028"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HCR5364521.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGMMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQSLISIVRLAMPLM"
     gene            complement(2854..4383)
                     /gene="nuoM"
                     /locus_tag="OO130_001029"
     CDS             complement(2854..4383)
                     /gene="nuoM"
                     /locus_tag="OO130_001029"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HCR5364522.1"
                     /translation="MLLPWLILIPFIGGFLCWQTEHFDVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4547..6388)
                     /gene="nuoL"
                     /locus_tag="OO130_001030"
     CDS             complement(4547..6388)
                     /gene="nuoL"
                     /locus_tag="OO130_001030"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HCR5364523.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEILHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYFCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQSVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWQGKRTLVTSIANSAPGRLLGTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6385..6687)
                     /gene="nuoK"
                     /locus_tag="OO130_001031"
     CDS             complement(6385..6687)
                     /gene="nuoK"
                     /locus_tag="OO130_001031"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HCR5364524.1"
                     /translation="MIPLQHGLILSAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(6684..7238)
                     /gene="nuoJ"
                     /locus_tag="OO130_001032"
     CDS             complement(6684..7238)
                     /gene="nuoJ"
                     /locus_tag="OO130_001032"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HCR5364525.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7250..7792)
                     /gene="nuoI"
                     /locus_tag="OO130_001033"
     CDS             complement(7250..7792)
                     /gene="nuoI"
                     /locus_tag="OO130_001033"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HCR5364526.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(7807..8784)
                     /gene="nuoH"
                     /locus_tag="OO130_001034"
     CDS             complement(7807..8784)
                     /gene="nuoH"
                     /locus_tag="OO130_001034"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HCR5364527.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(8781..11507)
                     /gene="nuoG"
                     /locus_tag="OO130_001035"
     CDS             complement(8781..11507)
                     /gene="nuoG"
                     /locus_tag="OO130_001035"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HCR5364528.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSLE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11560..12897)
                     /gene="nuoF"
                     /locus_tag="OO130_001036"
     CDS             complement(11560..12897)
                     /gene="nuoF"
                     /locus_tag="OO130_001036"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HCR5364529.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(12894..13394)
                     /gene="nuoE"
                     /locus_tag="OO130_001037"
     CDS             complement(12894..13394)
                     /gene="nuoE"
                     /locus_tag="OO130_001037"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HCR5364530.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13397..15199)
                     /gene="nuoC"
                     /locus_tag="OO130_001038"
     CDS             complement(13397..15199)
                     /gene="nuoC"
                     /locus_tag="OO130_001038"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HCR5364531.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15293..15955)
                     /gene="nuoB"
                     /locus_tag="OO130_001039"
     CDS             complement(15293..15955)
                     /gene="nuoB"
                     /locus_tag="OO130_001039"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HCR5364532.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(15971..16414)
                     /gene="nuoA"
                     /locus_tag="OO130_001040"
     CDS             complement(15971..16414)
                     /gene="nuoA"
                     /locus_tag="OO130_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HCR5364533.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(17045..17983)
                     /gene="lrhA"
                     /locus_tag="OO130_001041"
     CDS             complement(17045..17983)
                     /gene="lrhA"
                     /locus_tag="OO130_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HCR5364534.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            18902..20119
                     /gene="alaA"
                     /locus_tag="OO130_001042"
     CDS             18902..20119
                     /gene="alaA"
                     /locus_tag="OO130_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HCR5364535.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            20203..20802
                     /gene="yfbR"
                     /locus_tag="OO130_001043"
     CDS             20203..20802
                     /gene="yfbR"
                     /locus_tag="OO130_001043"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HCR5364536.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVLTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(20861..22693)
                     /locus_tag="OO130_001044"
     CDS             complement(20861..22693)
                     /locus_tag="OO130_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HCR5364537.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPVGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(22780..23430)
                     /gene="hxpA"
                     /locus_tag="OO130_001045"
     CDS             complement(22780..23430)
                     /gene="hxpA"
                     /locus_tag="OO130_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HCR5364538.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTCLEHIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPKVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     AEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQLNGDVIIQ"
     gene            complement(23441..23935)
                     /locus_tag="OO130_001046"
     CDS             complement(23441..23935)
                     /locus_tag="OO130_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HCR5364539.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACLRQYHLSANEINQ
                     IINA"
     gene            complement(24018..24473)
                     /locus_tag="OO130_001047"
     CDS             complement(24018..24473)
                     /locus_tag="OO130_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HCR5364540.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            24811..26013
                     /gene="ackA"
                     /locus_tag="OO130_001048"
     CDS             24811..26013
                     /gene="ackA"
                     /locus_tag="OO130_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HCR5364541.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            26088..28232
                     /gene="pta"
                     /locus_tag="OO130_001049"
     CDS             26088..28232
                     /gene="pta"
                     /locus_tag="OO130_001049"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HCR5364542.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNQEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            28422..29942
                     /gene="yfcC"
                     /locus_tag="OO130_001050"
     CDS             28422..29942
                     /gene="yfcC"
                     /locus_tag="OO130_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HCR5364543.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATMIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVSRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(29975..30517)
                     /gene="yfcD"
                     /locus_tag="OO130_001051"
     CDS             complement(29975..30517)
                     /gene="yfcD"
                     /locus_tag="OO130_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HCR5364544.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(30575..31126)
                     /gene="yfcE"
                     /locus_tag="OO130_001052"
     CDS             complement(30575..31126)
                     /gene="yfcE"
                     /locus_tag="OO130_001052"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HCR5364545.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(31182..31814)
                     /gene="yfcF"
                     /locus_tag="OO130_001053"
     CDS             complement(31182..31814)
                     /gene="yfcF"
                     /locus_tag="OO130_001053"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HCR5364546.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASVAMAEHLLALGQPNLFGEW
                     CIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            31950..32597
                     /gene="yfcG"
                     /locus_tag="OO130_001054"
     CDS             31950..32597
                     /gene="yfcG"
                     /locus_tag="OO130_001054"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HCR5364547.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGELGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            32654..33016
                     /gene="folX"
                     /locus_tag="OO130_001055"
     CDS             32654..33016
                     /gene="folX"
                     /locus_tag="OO130_001055"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HCR5364548.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            33037..33930
                     /locus_tag="OO130_001056"
     CDS             33037..33930
                     /locus_tag="OO130_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HCR5364549.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIDSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPATAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(33978..>34543)
                     /gene="rpnB"
                     /locus_tag="OO130_001057"
     CDS             complement(33978..>34543)
                     /gene="rpnB"
                     /locus_tag="OO130_001057"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HCR5364550.1"
                     /translation="GYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSA
                     FPLVDITVVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKA
                     LFNYVLQTGDAQRFRAFIGEIAERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQE
                     MLDRGLDRELVMMVTRLSPDDLIAQSH"
BASE COUNT         8662 a         9395 c         8641 g         7845 t
ORIGIN      
        1 tgtatagatc taacaaccgt aataaaacaa atactcataa ctcattataa ataatgataa
       61 aataacgtct tttattgata atttatatat gggaaaaatt ccttttgtta tattgtttct
      121 gatttcactc cctctgatta tttttaccaa ccctgggtca ctgcattacc accaacgccc
      181 cgacacgtca gtcctgagca attagaaaaa acagttcgtt atcttacaca aactgtgcat
      241 ccacgtaatg ccgacaatat tgataatctg aataggtccg tagagtatat aaaagagttc
      301 tttgtcagta gcggtgccag agttaccgcg cagggcgtcc ccgattggct attacgactc
      361 tgcgcctggt agccaggatt acctttaccc gcaatgtcct ggctttatcc cgatcggggg
      421 gactttattg cctggtcggc agaatacagg atatcaacgc cgttcgtcag gtaaaaacgg
      481 cattgttatc atcccgggat ttatctgttt attctatgaa tgccccatgg tttattcccg
      541 gtattgattt ctctgaccac ctgaattatt ggcaacacga tattcccgcc gtaatgatta
      601 ctgacaccgc tttttatcgt aataaacaat accacttgcc cggtgataca gcagacagat
      661 tgaattatca gaaaatggct cagatggtgt tataaccttg ttacacaaca gtaaataata
      721 aaaatttctc gaggcgggtc cagaaacagc gacatgtaca ttttattttc cctcgtctgt
      781 tttaagatgc gaaacataaa attcaatgga ggaaaaataa atgggaatga ttggttattt
      841 tgcagaaatt gactcagaaa agattaacca atctgcttga gtctactgaa aaacctttga
      901 tggataatat acatgacacg ctttcaggat tacgccgact ggatattgat aaacgctggg
      961 attttttaca ctttgggctg acaggcaccc ctgcttttga tcccgcgaag aatgatccgc
     1021 tgagtcgtgc cgtgcttggt gaacacagcc tcgaagatgg catagatggc ttcctgggat
     1081 tgacctggaa tcaggaatta gccgcgacaa tcgaccgact ggaatcactg gatcgaagta
     1141 agctgcgtaa acagttttcc attaagcgac tatgaaatgg agatttatcc gggagtgacc
     1201 ttttcagaag agttagaggg gcaactcttt gcctcgatca tgcttgatat ggaaaagctg
     1261 attagcgcgt atcgacgtat gctgcgacaa gggaatcatg cgctgacggt tattgttggg
     1321 tgatggtgtc agagcgccaa aaaaaaccgc cgaacatgtc ggcggttttc tgacttacaa
     1381 actaacagat tacatcagcg gcattgctaa ccgcacaata ctaatcagcg attgtggcca
     1441 tacacccagc accagtacca acagtgcaga aatcagcacc acgataccgc ccgcgctgta
     1501 ctgccagttt gacggtgcat cgcgacccgg ttgttccggt gcgtgcaggt acaggctcac
     1561 cgccacgcgc aggtagtagt agaggccgat tgccgaaccg acaaccacgg cacccaccag
     1621 ccaccacaag tgtgcctgga caccgaccgc cagcacgtag aacttaccga taaagcccag
     1681 cgtcatcggg ataccggcca gagacagcat catcaccgtc atcactgccg cgaggatcgg
     1741 acgatgccag aacagaccgc ggtaagagaa cagggaatca gcatccgggc cacgatacgg
     1801 gctggacatc aggctgacca cgccgaacgc gccgaggctg ctgaacagat aaccggccag
     1861 gtaaacccct accgcttcca tcgacatctc gccggtttgc agcgcaatca gcgccaccag
     1921 cagatagccg aggtgagaga tagatgagta accgagcaga cgtttgatgt tggtctggct
     1981 caacgccatc aggttaccga agatgatgga ggcaaaggcg ataatcgcca gtactacgcg
     2041 aatcgcttcg ctgtcaccca ccggtgcata gaggaacaga cgcatcacca caccgaagat
     2101 agcgattttg ctcgccgtcg ccaggaaagt ggaaaccggc gcaggcgcgc cttggtatac
     2161 gtctggcgtc cacaggtgga acggcaccag agagagtttg aagccgaggc caacaatcat
     2221 catgccgaaa cctgccagca acagcggctc gttgagcata ccgtcaccga ggtttttacc
     2281 cagcgcgaca aacgacaggt cgccagactg cgcatacacc agcgccatac caaacagcag
     2341 gaaagaagac gctgcggcag aaaggatggt gtatttgata ctggcttcca gcgaacgttt
     2401 ctggcggaaa gcgtaaccga ccaggccaaa cagcggcaaa gagatcagtt caataccgag
     2461 gaacagagac gccagatggt ttgcattcgc cagcaggatc ccgcccagcg cggcaattaa
     2521 caccaacagg tagaactcat ccttgttgtc gttatagcct tcaagccacg ggtaggcgaa
     2581 agtacaggtg gcgaggctcg ccaacaatac cagcccggtg taaagcatgg cgaaaccatc
     2641 aacgcgcatc agcggcgtaa cgtccatagc gcccgcctgg ccaacaaacc agagcgaaac
     2701 cagcgccgcg ttaagcccaa taaccgagag cgtagcgttg aggaaatgat tgcgtcgcca
     2761 cgcaatggag agcatcacaa ccaccaccgt taagccgacg atcagcaacg gtagcagtgc
     2821 gatcaggttt tgtggagtta ttgtcatggc gatttacggc cttgtagtag taacggaatt
     2881 aacaaaccac tgctggatat tgccaatcgc ggagtgcgag gtatccagaa tcggctgcgg
     2941 atagaagccc agcagtacca gcagcaccac cagcaacagg atcataaaca gctcacgcag
     3001 cgacatccct ggcagttcct ggctggcaat ctggcttttc gctttaccga agtaagcgcg
     3061 atgcaacatc gccagcgaat aaacagatgc aaagaccagc ccaaaggtag agataacggt
     3121 aatcaccggg acaacctgga agctgccgaa cagaatcata aattcgccga cgaagttacc
     3181 ggtgccaggc atcccaagcg ttgccaccgc aaagaacagc gacaatgctg gcagccattt
     3241 catcttgctc cacagaccgc ccatcatgcg catgtcgcgg gtatggatac gttcataaag
     3301 ctgaccacaa agaataaaca gacccgccgc cgacaaaccg tgcgcaatca tctggattac
     3361 cgcgccctgg taggccaact ggctgccggt gtagatagca atcagcacga agcccatgtg
     3421 ggaaaccgag gtgtaggcga tcagacgttt gatatcggtc tgggcgaagg ccatccacgc
     3481 accgtagaag atgccgataa cacccagcca catagcaatt ggcgcgaact ctgccgacgc
     3541 gttcgggaac agcggcaggg agaaacgcag caaaccgtaa gcggcagttt tcagcaagat
     3601 ccccgcgaga tcaacggaac cggcggtcgg agcctgggag tgcgcatccg gcagccagcc
     3661 atgcagcgga accaccggca ttttgactgc gaaggcgatg aagaagccca gcattaacag
     3721 gtattccaca ccactggaca ttggcgtatt cagcagctct tcatagttga aggtccagac
     3781 gccggtcgca ttgtagtgaa caaaaaccag cgccaggatg gcaatcaaca tcaccaggcc
     3841 actcgcctgg gtgtaaatga agaacttggt tgccgccgtg atacgcgttt taccgtcaga
     3901 ggctttatgc ccccacagtg cgatcaggaa gtacatcggc accagcatca tttcccagaa
     3961 gaagaagaac aggaacatgt cgatggcaag gaacacgccg ataacgccgc ccaggatcca
     4021 catcaggttg aggtggaaga agccctgata tttttcgatc tctttccacg aacagagtac
     4081 cgccagcaca ccgagcagac cggtcagcac gaccatcagc agcgacagac cgtcaatggc
     4141 gagatgaata gagataccaa aacgcgggat ccacggcatg tcgaattcag actgccactg
     4201 cggaattccg gcggattgcg tcagtgaata accgccctgc aaccacagtt gcagcgacag
     4261 cgccagcgtc aatcccatgg tgatcagcgc gatccagcgt ggcaccttga cgtcaaagtg
     4321 ttcggtctgc cagcacagga agccgccaat aaagggaatt aatattagcc agggtagtaa
     4381 catggcgatc tttattcctt gtaaaagtcc cgtcaggacc ggattttcaa cgaattctca
     4441 cgacagaaaa ttctcttcgg actgggggta gtgtcggatg cgccgcgaag gcagcttatc
     4501 cgacctacgt tcttatatgc ccaggagcaa tcccacaatc cttaactcaa cgcagtacca
     4561 tcaacagtgc cagcaccacg accgcaccga tgctcatgga tgccacatac cagcgcagat
     4621 aaccgttctc gcttaacagc aggcctttac ctgcaaagcg ggaaagaacc gccggaatgt
     4681 tcatcattga gttcagcgga tcgcgtttca gcaaccaggc aatacccaga aacggcttga
     4741 cgaacacttt gtcgtacagc cagtcaaagc cccaggcgtt gtaccaccag gtgcccagca
     4801 gacggcccgg cgcactgttg gcaatggagg tcaccagagt acgtttaccc tgccacagcc
     4861 aggctgccag cagaatgccg accaccgcga ccacgccaga ggtaatttcc agggtcaaca
     4921 tgctgccgtg cgccagttcc gtcgtttgcg gaagcacgct ctgcagcggc ggtacaatca
     4981 gtgcgccaac gaaggtggaa aggatcagca gcacaatcag cggcaggctg tgagttaccc
     5041 ctttcacggc gtgagcgtga atttgttctt ttccgtggaa gacgatgaaa atcatacgga
     5101 aggtgtagag cgaggtcata aacgcaccga ccagacctgc caccatcaga ttgatatgac
     5161 cattcgccat cgcacccgcg aggatctcat ccttactgaa gaagcccgca gtgaccagcg
     5221 gtagtgccga cagtgctgcg ccgcccacca ggaagcagaa ataaaccagc ggaatagatt
     5281 tacgcagacc gcccatcttg aagatgttct gttcgtgatg gcaggccaga atgacggaac
     5341 cggatgccag gaacagcagc gctttaaaga acgcgtgagt catcaagtgg aaaatcgccg
     5401 catcccatgc ctgcacgcca agcgcgagga acatgtagcc aatctggctc atggtagagt
     5461 aagcgagaac acgtttgatg tcggtctgta ccagcgcggc aaaaccggcc agcagcagcg
     5521 taaccgcccc gacaatgccc accagatgca gaatttccgg cgtcatcagg aacaggccgt
     5581 gggtacgggc gatcaggtag acacccgcgg ttaccatggt tgcggcgtgg atcagcgcgg
     5641 agacaggcgt cgggcccgcc atcgcgtcgg caagccatgt ctgcaacggc aactgcgcag
     5701 atttaccgac cgcaccgccc agcagcatca gcgtcgccca catcagcatg ttattgccgt
     5761 cagcaaagtg cgctggtgcc agttccacca tttcgcggaa gttcagggtg cccagttcgt
     5821 tgtaaagaat gaacagcgcg aaagcgagga acacgtcacc cacacgggtc acgacgaacg
     5881 ctttcattgc cgctgcgcca ttcttcggat cggtgtaata gaacccgatc agcagatagg
     5941 agcacaggcc cacgccttcc cagccgaggt acatcagcag caggttatcg gcgagaacca
     6001 gaactaccat gctggcgatg aacaggttgg tgtaggcgaa gaagcgagag tagccctctt
     6061 caccgcgcat gtaccaggag gcgtacatgt gaataaggaa acccacacca gtgaccaccg
     6121 agagcatggt cagcgacagg ccgtccagca ccaggttaaa accgatgtta aagtcgccta
     6181 ccgacatcca cgtccacagc ggctggctgt atgcctgctc gccgttagcg aagaaatcaa
     6241 cgccgataaa ggcggttacc agcgccgcca ggcccacaga gccaacgccg acgatcgccg
     6301 aaacgttttc agaccagcgc ccacgggaga acgccagcag gacgaagcca atcaatggca
     6361 aaataatggt taaggcaagc atgttcatcc gcgcatctca cttactgaat cgatgttcag
     6421 gttctggcga cgacggtgaa gttgcagcag cagcgcaagg ccgatactcg cttctgccgc
     6481 cgcgaggctg atggcgagaa tgtacatcac ctgaccgtcg gtctgacccc agtagcttcc
     6541 ggcgaccaca aaggccagcg cggaggcgtt aatcatgatt tccagaccaa tcagcataaa
     6601 cagcagattg cgacggataa ccagaccggt taagccaaga acgaataaga ttgccgagag
     6661 gatcagtcca tgttgtaagg ggatcatgcg tgctcctccg tttttctttt cgcgctgtcg
     6721 tctttacgat tgctcagcac ttcacccgca cgctcttcac gaccgacgtg gaaggccaca
     6781 accagacccg cgagcagcag catagaagcc agttccaccg ccagtacgta aggcccgaac
     6841 agcgtaatac ccactgcttt agcactgatt ggcgtaccgt cgataccctg atcattaaca
     6901 ccaaggatgg cgtaaacaat caccaccagc atgatggccg acaaaattgc cggaccaatc
     6961 cacacctgcg gtttcagcca ctggcgttcc tgttcgattt ctgaaccgcc caggttgagc
     7021 atcatcacca cgaacacgaa cagcaccata atggcacccg cgtagacgat aatttccagc
     7081 gcaccggcga agtaagcgcc cagtgagaag aacaccccgg aaatcgccag cagcgaaata
     7141 atcaggtaca gcagtgcgtg taccggattg gtatgggtga tcactcgcaa ggtcgcaagt
     7201 atggctatca ggccacagat ataaaaagcg aactccattg cccctctcct tacggtaaca
     7261 ggctcttgac gtcgataggc ttggcttcgt tctctgcttc gcccttatct ttgccgtcga
     7321 ttgccatacc tgccatccgg tagaagttat attccgggta tttgcccgga ccggagatca
     7381 gcagatcctc tttttcgtaa accagatcct ggcgcttgta ttcccccatt tcgaaatccg
     7441 gggttaactg aatcgccgtg gtcggacagg cttcttcgca cagaccacag aaaatgcagc
     7501 gtgagaagtt gatgcggaaa aattccgggt accagcgacc gtctttggtt tctgcttttt
     7561 gcagcgagat acagccgacc gggcaggcta ccgcgcagag gttacaggct acgcaacgct
     7621 cttcgccgtc cgggtcgcgg gtcagaacga tacgaccacg ataacggggc ggcagataga
     7681 ccggctcttc cgggtacatt cgcgtttcgc gtttggcgaa cgcgtgcagg ccgatcatcc
     7741 agatactacg aacctgggtg ccgaaaccta ctaacaattc ttttaaggtc atggtcttat
     7801 tgccccttat tgcgcctgcc agagaatgac agccgccgtt accagcaagt tgatcagcgt
     7861 cagcggcagg cagattttcc agccgaagga cattacctgg tcataacgcg gacgcggtaa
     7921 cgacgcacga atcaaaatga acatcatcat aaagaacgcg gttttcaacg cgaaccagat
     7981 gaatggcggt aacaacgggc cttgccagcc accgaagaac agcgtcacca tcaatgcaga
     8041 gatggtcaca atcccgatgt actcacccac gaagaacaga ccgaacttca taccggaata
     8101 ttcaatgtgg taaccatccg ccagttcctg ctcggcttcc ggctggtcaa acgggtgacg
     8161 gtgacatacc gccacgcccg cgatggcaaa ggtaataaaa ccaaagaatt gcgggataac
     8221 gttccacaca tgcgcctggc tgttgacgat gtcggtcatg ttgaatgaac cggcctgcgc
     8281 caccacgccc atcagggaaa gcccgaggaa cacttcgtag ctcagggtct gcgcagaagc
     8341 acgcatcgca cccagcaacg agtatttgtt gttacttgac cagcccgcaa acagcaccgc
     8401 gtaaaccgcc agacctgcca tcatcaggaa gaacaaaatc ccgatgttca ggtcggcaac
     8461 cacccaaccc ggactgactg gcacaatcgc aaaggccagc agcagcgagg taaaggcaat
     8521 catcggtgcc agggtaaaga tgacgcgatc cgagaatttc gggatccagt cttctttaaa
     8581 gaacattttg atcatgtccg caaccagctg gagcgaaccg ccccagccaa cacggttagg
     8641 tccataacgg ttctggaaca gacccagcag gcgacgttcg ccaaagctca tgaatgcccc
     8701 acaggtgaca accaccagca ggatcaccac cgctttgagg atggtcagca ggatctcaat
     8761 cagttccggt gatatccaac tcattgttgt gcctccttga gatcctcaag atgcgcgcca
     8821 gccagcaccg gagcaatgcc ggacataccc atcggcaagc ccacctgccc tgccgtcagt
     8881 ccttcggcga tttcaaccgg cagcgtgacc gtgttgccat cgtaactaaa ggagacgcgt
     8941 gtacctgcgt tcacacccaa cttcgcggca tccgctgggt tgagtttgat gtacggctgc
     9001 ggcatacggc tctggaagac cggagcacgc tgtgacaatt catcgctgcc aaacaggtgg
     9061 taatacggcg caatacgcca tttcccgtcc tgcggctgga agcgtgccgg tacgctggtg
     9121 aagtaatcca gaccattctc gctggtttca aacagacgca cgcccggatc gccaaagcgc
     9181 agtttgccgc ccacttcgtc ctggaatttg ttccacgcct gcggggagtt ccagcccggt
     9241 gcccaggcaa acggcacttg cgaacggtgc gcggtcggct ggttgttacc ttccatcgag
     9301 aaggtgaaca tggtgtcaat atcctgcggc tgacgcggct catgaacgct gatattggcg
     9361 cgcatggcgg tacgaccgct gtaacggtgc ggttcacggg ccagtttctg cccacgaata
     9421 cggaatgtcg catccggcgc agcatctttg atacctgcca gttccgggat tttcgccaca
     9481 acagcgtcaa tcacatggtc gagctgcgtc cagtccactt cacggctcag cagggtgctg
     9541 tgcagcgagt gcagccagcg ccagctttcc agcatgacag ttttgctgtc gtaataagcc
     9601 ggatcgtaaa cctggaagaa acgttgggcg cgaccttcat tattaattac cgtaccgtcg
     9661 ctttcagcaa agctggcggc ggaaagtacc agatgggcgt tttccatgat cgctgtacgc
     9721 tgatggtcaa ccaccatcac cagcggtgct ttagccagcg cagcattcac gcgggtagca
     9781 gaagcgtgac gatgcagatc gttttccaac accaccaccg cgtcggcgcg tccggtttcc
     9841 agttcggtta acgcttcttc aagcgaaccg ccacccataa tgcccagccc catgctgttg
     9901 acggaacggg caatcatggt gataccgacg tcagcgccgc gacctttcag ggctttcgcg
     9961 acgttagccg ccgcctgaat cacctctaag ctaccggcgt tcgtcccgga gataatcaac
    10021 ggtttcttcg cacctgccag tgcctgcacg atgacgtcga ttttgctttg cagctcaggt
    10081 tcgataccgt caaccgctgg tgcagagtta tccagcgcat gggcgatggc aaaacctaaa
    10141 cgcgcctgat cttcaaccgg tgcgcggtaa gtccacgccg cgatatcatc cagacgggtg
    10201 tcatcaacgt tggtaacaaa cagcggatgc ttcgcacgtt gaccgatgtt aaggattgcc
    10261 gcaatctgcc agtcagccac tttctgtgct gccgccattt cgcgcgcttt acctttcacc
    10321 gcctgacgca ctgccagcgc gacgcgcgcg ccggtctggg taacgtcttc acccagcacc
    10381 agtaccgcat cgtaagattc gatttcgcgc agagccggag tataaatgcc gccttcacgc
    10441 agcactttca gcgccagttg cagacgttcc tgctcaccgt gagcgatacc ggtgtagaag
    10501 ttttcttcgc ccaccagttc acgcagtgca aagttgcttt ccacgctggc acgcggagaa
    10561 ccaataccga tcactttctt cgactgacgc agaatatctg ccgcgccctg cattgcctgt
    10621 tcggcgttga gggtaatgaa atcatcgcca cgacgctgta ctggctgacg cggacgatcc
    10681 ttcaggttga cgtaaccgta accgaaacga ccacggtcgc agaggaagta gtggtttacc
    10741 gtaccgttgt aacggttttc gatacgacgc agttcgccgt aacgttcacc ggggctgatg
    10801 ttacagccga tggaacattg ctggcagatg ctcggcgcaa actgcatatc ccatttacgg
    10861 ttgtaacgct cggagtgcgt tttgtcggta aatacgccgg tcgggcaaat ttcgaccagg
    10921 ttaccggaaa attcgctttc cagcgtgccg tcttccgggc gaccgaagta gacgttgtcg
    10981 tgcgcgccat aaacgcccag atctgtaccg tcagcgtaat ctttgtagta acgcacacag
    11041 cggtagcagg cgatgcagcg gttcatttcg tgagatatga atggccccaa atcctgatta
    11101 cggtgggtac gtttggtgaa acggtagcga cggaagctgt gtccggtcat cacggtcata
    11161 tcctgaagat ggcagttacc gccttcttca cataccggac agtcgtgcgg gtggttggtc
    11221 attaaccact cgaccacact ttcacggaac tgtttcgctt cttcgtcgtc aatggaaata
    11281 aaggtgccat cggaagccgg tgtcatacag gacatcacca ggcgaccacg cgtgtcttcc
    11341 gcgttttggt attgcttcac cgcacactgg cggcaagcac cgacgcttcc cagcgccgga
    11401 tgccagcaaa agtaaggaat atcaaggccc agagacagac aagcttccag caggttgtcc
    11461 gctccgttga cctcgtattc tttgccgtct acatgaattg tagccattag catgcttcca
    11521 gttttctcgg tcagggaccg agcgttaatc gaaattcggt taccagcgct ctttcagcag
    11581 gttcggctga atcccattaa tcaaatgggt attgctgaac ggctgtttga ttcccgcctc
    11641 aaattcttcg cggaaatatt tgatggcgct ctgtaacggc tccactgcac caggtgcgtg
    11701 ggcacagaaa gttttacccg ggcctaagaa tcgacacagt tgctcaagtg tttcaatatc
    11761 gcccggctgg ccttcgccac gctccagcgc acgcagaatt ttcacgctcc acggcagacc
    11821 gtcgcggcac ggcgtacacc agccgcagga ctcacgggcg aaaaactctt ccaggttacg
    11881 caccagcgac accatgttga tctcatggtc aaccgccatc gccagcgccg tacccagacg
    11941 gctgcccgct ttaccgatac tttcgaattc catcggcaga tcaaggtgcg cttcggtcag
    12001 gaagtcagtc cccgcgccgc ctggctgcca ggctttaaat ttcagaccat cacgcatacc
    12061 accggcgtaa tcttcgagga tctcgcgtgc ggtggtgccg aacggcagtt cccacagtcc
    12121 cggatttttc acccgaccgg agaagcccat cagcttggtg ccagcatctt tacttttcga
    12181 gatgttctga taccactcca cgccgttagc gaggatcgcc ggaacgttac acagggtttc
    12241 gacgttgttg acacaggtcg gtttgcccca tacgccggag gttgccggga aggggggttt
    12301 cgagcgtggg ttagcacgac gcccttctag ggagttgatt aacgctgttt cttccccgca
    12361 gatgtagcgc cctgccccgg tatggacgaa cagttcgaag tcgaaacctg ttcccataat
    12421 gtttttgcca agcaagcccg cttcagtggc ttcggcaatg gcgcggcgca gatttactgc
    12481 cgcttcgata tattcgccac gcaggaagat gtagccacgg taagctttca gcgcaaacgc
    12541 ggagatgagc ataccttcca ccagcaggtg cggcagttgc tccatcaaca ggcggtcttt
    12601 ataggtgccc ggctccattt catcggcgtt acacagcagg taacggatgt tcatggattc
    12661 gtctttcggc atcaggctcc acttcaggcc agtagaaaag cccgctccgc cgcgcccttt
    12721 cagaccagcg tcttttacct gattaacgat ttcgtccgga gacagcccgg tcagcgcctt
    12781 acgcgcccct tcgtaaccgt ttttgctgcg gtattcgtcc agccacactg gctgtttgtc
    12841 atcgcgcaga cgccaggtca gcggatgcgt ttcgggagta cggataatgt ttttcattta
    12901 taccgctcca gcagttcagg gatcgcttcc ggggtcagat gcgcgtgagt gtcctcatcg
    12961 atcatcatgt ttggcccttt atcacagttc cccaggcagc aagttggcag cagcgtaaag
    13021 cggccatcaa atgtcgtttg ccctggtttg atgttcagct ttttctcgag cgccgcctga
    13081 ataccctgat acccgttgat atgacagacc acgctgtcgc aataacggat cacatggcga
    13141 ccaaccggct ggcggaagat ctgactgtag aacgttgcca caccttcgac gtcgcttgcc
    13201 ggaataccca gcacatcggc gatcgcgtgg atcgcaccat ccggcaccca gccacgctgc
    13261 ttctgaacga ttttcagcgc ttcaatggac gccgcacgcg ggtcttcgta gtggtgcatc
    13321 tcgtgctcaa tcgcttcacg ctctgccgca ctcagctcaa aagcctcggt ttgtggttgt
    13381 tgattctcgt gcataattag cggtccacat ctgacataac aaaatcgata ctgcccagat
    13441 aaacaatcag gtcagacacc aggctgccgc ggatcgccgc cggaatttgc tgcaaatgcg
    13501 cataactcgg cgtgcggata cgagtgcgat aactcatggt gctaccgtcg ctggtcaggt
    13561 agtaactgtt gatccccttg gtcgcctcaa tcatctggaa agattcattg gcaggcatca
    13621 ccggccccca cgacacttgc aggaagtggg tgatcagggt ttcgatatgt tgcagcgtgc
    13681 gctctttcgg cggcggcgtg gtcagcgggt gatccgcttt gaacgggcct tccggcatgt
    13741 tgttgaggca ctgctcaaga atgcgcagac tctggcgcag ctcttccact ttcagcatta
    13801 cgcgggtgta gcagtcagaa acaccaccac caaccgggat ttcaaagtcg aagttttcat
    13861 agccagaata aggacgcgcc ttacgcacgt cgaagtcgat cccggtagca cgcaggcccg
    13921 cgccagtggt gccccactcc agcgcctctt tcgcgccata ggcggcaacg ccctgggaac
    13981 gacctttcag aatggtattt tgcagcgccg ctttctcgta agacgccaga cgtttcggca
    14041 tccagtcgag gaactcacgc agcaggcgat cccagccgcg cggcaggtcg tgcgctacgc
    14101 cgccaatacg gaaccacgcc gggtgcatac ggaaacccgt gatcgcttcc accagatcgt
    14161 aaattttctg acgatcggta aaggcgaaga acacgggcgt cattgcgccg acgtcctgaa
    14221 taaaggtcga gatgtacagc aggtggctgt tgatacggaa cagttcggag agcataacgc
    14281 gaatgacgtt aacgcgatcc ggcacggtga tcccggccag tttctctacc gccagcacgt
    14341 aaggcatttc gttaacgcag ccgccgaggt attcaatacg gtcggtatac ggaatgtagc
    14401 tgtgccagga ctggcgctcg cccattttct cagcaccacg gtggtggtag ccgatgtctg
    14461 gtacgcagtc gacaatctct tcgccgtcga gttgtaaaac gatacggaaa gccccgtgcg
    14521 ccgacgggtg gttcggaccg aggttgagga acatgaagtc ctcgttttcg gtgccgcgct
    14581 tcatccccca ctcttccggt ttgaaggtca gggcttccat ctccagatcc tgtttggctt
    14641 tggtcagctc aaacggcgag aattcggtag cgcgcgccgg gtaatcttta cgcagcgggt
    14701 gacctttcca ggtttgcggc atcatgatgc ggcgcaggtt cgggtgaccg tcgaaagtaa
    14761 tgccaaacag atcccaggtt tcacgctcat accagttagc gttcgggaac agtttggtga
    14821 aggtcggtac gtgcaggtcg ttttctgcca gcgccacctt cagcatgatg tcgcggttac
    14881 gatcgataga aatcagatgg tagaaaacgg aaaaatccgc ggcaggtaac ccttcgcggt
    14941 gtgtgcgcag acgttcgtcc atgccgtgta agtcaaacag catgacgtaa ggtttcggca
    15001 gtttctttaa gaaatcgcca acttccagta attgttcacg cttgatccac acaacgggaa
    15061 ccccggtgcg agtcgcctga acagtaaagg catccggccc aaaacggttg cgcagttcgc
    15121 caattaccgg atcatcaaga tgatcgcggg tctgccaggc gggttcttgc gcggttaagt
    15181 cggtcatatt gttcaccatt gcaaatggtc cgtggtgact gtcggcgcag taagcttcgc
    15241 gaaatagatt taggaataag cgaagaaaaa tccctttgcc gacaggcgca aattaaatct
    15301 cgtcaggtgt acgcaggttg gttacggcaa tgcgttcacc gcgcttgcgt tcgcgctctg
    15361 attgcatatt ggcgcgataa acgccctgat cgccaaccac ccaggagagc ggacgacgtt
    15421 ctttgccgat agattcctgc aacagcatca gtgcctgcat gtacgcttca ggacgcggcg
    15481 ggcagcccgg gatatacaca tcaaccggga tgaatttatc gacgccctgc acaacggaat
    15541 aaatatcgta cataccacca gagttggcac aggcacccat tgagataacc cattttggtt
    15601 ccagcatctg gtcatacaga cgctgaataa ccggtgccat tttggtaaag caggttcctg
    15661 caaccaccat caggtcagcc tgacgcggcg aagcacgcaa tacttctgcg ccaaaacgcg
    15721 ccacgtcatg caccgcggta aacgaagtca ccatctcaac gtaacagcag gaaagaccga
    15781 agttatacgg ccaaattgag tttttacgac cccagttaac catgtcattg agcttgccca
    15841 taaacacgtt tttgttaact tcttgctcca gagggtcggt tacgatctcc tgcttttgca
    15901 gggggtaacg gtcgttctca ccgttgggat ctatgcgggt gagcgtataa tccatcttaa
    15961 tgcctcgcgg ttagcgttga cgattagcga tactgttcgt ttccgggttc atacgctcgc
    16021 ggcgtgaacg cgcgggcgtc cagtccagcg cgccaatacg caccagataa accagacctg
    16081 ccagtaacac aaaaataaaa attgcagctt ccacaaagcc tacccagccg ctttcgcgga
    16141 tagaggttga ccatgcgaac agatacagcg cttcaacgtc gaagataacg aagaacatgg
    16201 ccaccagata aaacttggcg gacaggcgta agcgggcgga gccgaccgag tcgataccgg
    16261 attcaaacgg cacgtttttc gacctcgcgc gtgcgcgacc gcctaaaaac caaccaccta
    16321 ccagcattag gcaacacagg ccaatggcaa cgataagaaa gatagcgaat gcccagtgat
    16381 gagcgatgac ttcagtggat gttgacatac tcattgctta ctcatcaaaa gtagcgccag
    16441 attcactgct cttcacggca gatggacgcc acatcgattc atggggagga ataaaaaaaa
    16501 ccttacaatc actgtagaaa ttcttttata cagctaattg atgtggtctt ttactccttt
    16561 ctataacctt ttgtcaactt taacaaaagt ttcttcacat tagtttacat aatatcaaca
    16621 ccattagcat ttaatgccct ttcaccccag ctccttgacg actccaggat aattagatgt
    16681 tgttgaatcg tgtccgttgt gaagcaatgg aaaaaatacg ggtctatttt gacaggaatt
    16741 tgtgtcgatt cctcccccca aaagagagta ttttcttgat ctgtgacacg cttttgtcat
    16801 tccataacaa aaacgcagca acaaatttac gtatttttta acatcattgt agcaggtgat
    16861 ttttttcagg cgattatttg tgcgttcggg acgtgaatct ctggtgatta aaaaatgaac
    16921 agttttgtac gttctgcact atgcgaggaa ggatttttac taaaaaaaag ccgctggggt
    16981 ttaaaacacc cccagcggct cgttttttac actattgtct caggaattat ctatcgtccg
    17041 tcgattactc gatatccctt tcaatcaaca atgaatcatc cccttccgga gcagacattg
    17101 ggctgtattg ccacggatta tggtagctct ccatcgcctg atagatcacc tgggccagct
    17161 cattattact ggacgggtca tagcacagca aatattcggt atcaggtagc ggcggtaagc
    17221 catctactcc actcagcaca cgcaggtccg ggctcatcat ctcaaccggc cttgccgtca
    17281 cgccaagacc ggctttcact gccgcacgaa cggccggaag cgtcgaggcg acataagcca
    17341 gtcgccatgg aatatctgct ttattaagcg ttgccagcac catatcgcga aacggacttg
    17401 gatcatccag caatacaaga gggatcggct cgcctttttg caatacgtat tccgctgcgc
    17461 agtaccagtg tgttggcgag gtacgcaggt tcaatgcctt aaacgctgaa ggacgatggg
    17521 tggttaccat caaatccact tcctgggatt ccagcatttc tgccatatag gcattacgtt
    17581 taacgcggac atccagcgct aatttcggat aaaccgaact cacgcgattt aacaggaaag
    17641 gtaagatcgt atcggcagat tcatctgaag caccgatagt taacacgccc tgaagattac
    17701 tgaacattaa tgatgagcag gcctcatcat taaaacgcag gattttcctg gcgtaaccaa
    17761 gaagttgaat gccatgttca gttaacagtt tgttgcgacc gtgacgagcg aacagttctt
    17821 tcccaacgag ttgttccaga cgctgcattt gctgacttac ggcggactga gtacgacaca
    17881 cagcggcagc tgcggcagca aaagtgttca gatcggcaac agcaacaaat gttctcagca
    17941 gatcgaggtc gaggttaatt atcggacgat ttgcacttat catatattat cacttactgg
    18001 cggctcatac tgagctggtt aatgctgtgc acacacaaac aagcaattcc atttgtaatg
    18061 tgcctccctg gcagtttcat ccggaacccg gacgaaagta aaaatgcata tgagttgcac
    18121 taaaaaagcg actcacattg ttccgttata atgcctgaag tagatcacag aatatatctt
    18181 cagggatcgc atatctatta agttactcac tcttttctat ttatgacatg cgcgtgtttg
    18241 tataaatgta aatgtgagtc cttgttccac tctcgtgcag catcgctggt catacgcgaa
    18301 cacgtacaaa cagcaatggt gaggcggcat caagagcagg gatccgtcat ttatgcgagc
    18361 attttacccc aaaaaccttt tatttataag ggccattgcg aatcatctga tgcaaagtta
    18421 tgttatgtta ggcaaagtaa tcttctatta ttaataagca catcaaaacc tttttgaata
    18481 ttaaataata attaattagc atcatcctca ttcattaatt ccgcttaaca atagtttcac
    18541 accttgcccc attacgacac ctcggaaatc atcacgctgt taaactctcc gtaatgcgga
    18601 aatatcatca tcgcgtttca ttagtgaatt cttaaatgag gcattttcac acaattatct
    18661 tacagataaa aaaaccagac ttacaattaa gaatcagaac aattcactat ataacattgc
    18721 atgtaaagca tatacacctc attattttgt catcattaag ttattaacag cacaatcgag
    18781 ccttcccctc tggcaaaatc ttattctgca gaccttcaaa acaccgtcct gggggagtac
    18841 attgttctaa gctgacttcc acggcaggga gtggcgataa cagcaaaaaa ggtcaagatt
    18901 catgtccccc attgaaaaat ccagcaaatt agagaatgtc tgttatgaca tccgtggtcc
    18961 ggtgctgaaa gaagcaaaac gcctggaaga agaaggtaac aaggtactga aactgaacat
    19021 cggcaaccca gccccgttcg gttttgacgc gccagatgaa atcctcgttg acgtgatacg
    19081 caacctgcct accgctcaag ggtattgcga ttccaaaggt ctttactccg cgcgtaaagc
    19141 catcatgcag cactaccagg ctcgtggcat gcgtgatgtt accgtggaag atatttacat
    19201 cggcaatggt gtatcggagc ttatcgttca ggcaatgcag gcattgctga acagcgggga
    19261 cgaaatgttg gttcctgcac cagattaccc actatggacc gcggcggttt cgctttccag
    19321 cggtaaagcg gtgcattatc tttgcgatga atcctctgac tggttcccgg acctcgatga
    19381 tattcgcgct aaaattacgc ctcgtacgcg tgggatcgtt attatcaacc caaataaccc
    19441 aaccggcgcg gtatattcca aagagctttt aatggagatt gtggagattg cacgtcagca
    19501 taatctcatt atcttcgccg atgaaattta tgacaaaatt ctttacgacg acgctgagca
    19561 tcactcaatt gcgccgctgg cacctgacct gctgaccatt acctttaacg gactgtcgaa
    19621 aacgtaccgc gttgcaggct tccgtcaggg gtggatggtg ttgaacgggc cgaaaaaaca
    19681 cgccaaaggc tacatcgaag gtctggaaat gctggcttca atgcgcctgt gtgctaacgt
    19741 tcctgcgcaa cacgccattc agaccgcgct gggtggttat cagagcatca gtgaatttat
    19801 tacccctggc ggtcgtcttt atgagcagcg taaccgcgcg tgggaactga tcaacgatat
    19861 tccgggcgtt tcctgcgtga aacctcgtgg tgcgctgtat atgttcccga aaatcgacgc
    19921 caaacgcttt aacattcacg acgatcagaa aatggtgctg gatttcctgt tgcaggaaaa
    19981 agttctgttg gtgcaaggga cggcattcaa ctggccgtgg ccggatcact tccgcattgt
    20041 cacgctaccg cgtgtcgatg atatcgagct gtctttgagc aagttcgcgc gtttcctttc
    20101 tggttatcat cagctgtaat cttaatttca ctgccggaga ttgcatccgg cagcgttatc
    20161 ccgccacaat gacctgatga tgtcatcata cgtaaggtca ctatgaaaca gagccatttt
    20221 tttgcccatc tctcccgcct gaaactcatt aaccgctggc cgctaatgcg caacgtgctg
    20281 acggaaaatg tgtccgaaca cagtttgcag gtagcgatgg tcgcccatgc gctggcagct
    20341 atcaaaaatc gaaaatttgg cggtaatgtc aacgccgaac gtatcgcttt actggcgatg
    20401 taccacgatg cctcagaagt gctcaccggc gatctcccta ctccggtgaa atacttcaat
    20461 tcgcaaatcg ctcaggagta caaggctatt gaaaaaatcg ctcagcaaaa actggtcgat
    20521 atggttccgg aagagctgcg ggatatcttt gcgccgttaa ttgacgagca tgcatatagc
    20581 gatgaagaaa aatcgctggt gaaacaggca gatgcactgt gtgcatatct gaaatgtctg
    20641 gaagaactcg cggccggaaa taatgaattc ttgctggcaa aaacgcgact ggaagcgacg
    20701 cttgaagcgc gtcgcagcca ggagatggac tacttcatgg aagtatttgt tcccagcttc
    20761 catctttcgc tcgatgagat tagccaggat tcaccgctgt aagcagccgg agtctgcgtc
    20821 gcatcaggca ataagcgccg gatgcgacat caggctcttg tcaaaacgga aacagcatcg
    20881 ggatcatcac cacacaaacc gccatcacga taatggtgaa cggtaccccc aacttcacaa
    20941 agtcactgaa gctgtaattt cccggaccta aaaccagtgt gttaacaggt gaagaaaccg
    21001 gtgtcataaa ggcggcggat gctgccatcg ccacgaccat cgcgaatgga taaggcgaca
    21061 cccccatcgt tttggcagca gccagcgcaa tcggagccat caacaccgcc gtcgcggtat
    21121 tagagataaa tagcccaata accgccgaca agacaaacaa acaccccagc atcatatgtg
    21181 gcccgtaacc accgccaatg tccatcagcc ctttcaccgc cagcgcgaca cctcccgttt
    21241 tctgtaatgc cacagcaaac ggcatcatcc caacgatcaa aataatgctc ggccagtgaa
    21301 tggatttata ggcgctttca gcatctatac agcggaattt ccccatcagc aggcaggcga
    21361 tgatagcggc aacaggatta ggaatttcat ctgtcagcat taacgccacc attagcacca
    21421 gacagaaaat ggcatggggt gcctggctgt gcgcgggcga tgcttcactc acctcttccg
    21481 gtaagttcag cgctacgaag tcgcggccct gtttggccag cataccgatc agtttccagt
    21541 tacccacaac caggatgata tcgcccagca gcagaggctc atccgccagc gaaccttcca
    21601 gcgccacgcc attgcgcttt agccccacca cattcagtcc gtagcgggta cgaaaaccaa
    21661 tttcgcgcac cgatttacca atcagttctg actcaggaat taatgaaatc tctgccatgc
    21721 ccacatcaag ggcctggtca gaaaaatact cgccgcgcag taccatcggc tccagcaatt
    21781 gctcactaca aaattgccgg agatcaacat cagccgcaga catatcaata agcaaaacgt
    21841 cacgcgcgcg aaattcagaa accccattaa cgttcacgat aacgcgacga aaacgccgcc
    21901 agcgttcaac accgatgacg ttagcgccat aacgctcacg taatttgaga tcatccagcc
    21961 gttgaccaat cattggcgat ccggggcgaa tagccagacg tcgcgcacgc ccggtcagtc
    22021 gatattcacg gataagatcg cgaaaggttc gacgcgtcca gccttcgcgc tgcggggtct
    22081 gggtatcccc tttcagcatg aaacgcatca ctaacatata caaaataccc agcaccagca
    22141 cgaccaggcc aacaggtgtt acgctaaaga aactgaagcc gtgatagcct tcacgcagca
    22201 attcactgtt gactaccagg ttcggcggcg tcgccaccag cgtcatcatg ccgctaatca
    22261 gcccggcaaa acttaacggc atcatcagac gtgacggcga agtttgcata cgcatggcaa
    22321 cgcttaacac cacgggaata aagatagcga caacgccggt tgaactcata aacgcgccaa
    22381 gccccgcgac ggtcagcatc aacaaaacca acattttgat ttcactgttg cccgccactt
    22441 tgaccagcca tgttcccatt acggtggcaa caccggtacg gaccaaacca tcgccaataa
    22501 taaacaaggc ggcaatcagg acaacgttag gatcagaaaa gccggaaaat acttcgggga
    22561 ccgtcagcgt tccgcttaat gcaaacgcga caataacaaa caaagcgacc gcatccatac
    22621 gcactctgcc cgtcgcaaac aagacgatgg caacggccag taatgaaaga acccaaatca
    22681 attcaccgtt cacaacttat ccttgttaat tgagggggat gacttgattc tgccataaaa
    22741 aagccccgac gagacggggc taaatcatga tcaggtgttt cactgaataa taacatcgcc
    22801 atttagctgt ttggtcacag taatttgctc cagactgtgg aggaccaaat cgacctcatt
    22861 caggcgcggg gtatctgccg gagcgttaac cgcaatgaca tggcaacccg ccgccaggcc
    22921 agaaagcacg ccagcgggag catcttccgc caccacacac tcctgcggcg caagccccag
    22981 cagctgcgca cctaacagat acgcatcagg ttctggtttt ccgcgcttca ctcgctcagc
    23041 ggttacaaac acctttggtg cgggaagccc agctatttta tggcgcgctc gcgctaccgg
    23101 catggagcca gaagtcacaa tggcccacgg aatacctgct ttattcaaat gattgagtaa
    23161 ggcgattgcc cccggaagcg cggtaatacc ttcggtttcc gtggcctcga tgtgctccag
    23221 acacgtaaac tcggcggcaa tatcagcctc ggatttgccc gccataaaat ggcgcaaaga
    23281 ggtgatcgcc tgtttaccgt gaatgaaagc cagcacctct tccggcgcta acccatgacg
    23341 tctggcccag ttgctccacg cccgttctac cgcaggcagg gaatccacca gcgttccatc
    23401 aagatcaaac agaaaacctt tgcaccgcac gcgggcctcc tcaggcgttg ataatttgat
    23461 taatttcgtt ggcgctcaaa tggtactgac gcaggcaggc atgccacaca ttaagcatgc
    23521 gctgatattt ttcccacatt ggcgtctggg cgttaaaacc atgagtccca gcgtcaaaat
    23581 gggtatagcg cccttccaca ttaaccataa agcggacata accgaggtaa cgtgcttcag
    23641 tggcggcgtc aaagccgagg aaggtgacac gacgttcatc gatggattgc tggtcctgca
    23701 aattagacca ggaaacatgc aacgcatgat acatctccat aatgtcgatg atagtgcggc
    23761 aggtttcttc tttcagctcg ccaaactcgc gatccaattc acgcatctgt aatccgtaac
    23821 cacgctcaat aattgtttgc aggcgacggt aacgttcagc atttgccgga tcgagcatag
    23881 tcatcatctt gtactgatta gacaaaataa gacgttgcgc gttggtcatt tccattgttg
    23941 actcctgtat cactctacta cggtgaaaaa aaagaaggct gagtatgcct tcttttatat
    24001 gcgtaatcag gggtcaatta caaatcatca aggaaagttt tatccagttg tttgaaggcg
    24061 cgcttaagcg tgtcagctaa tgcctggtaa tcaggcttgc cttcaacggg tgccaacacc
    24121 tgtccagact cctgcaattt accgcgaact tcataaaacc agttgaggat agcagggggt
    24181 aatggcgtta cagaacgctt gcccagccac cataatccct gcatgggtaa acttaaggcg
    24241 aacagcgcag tggcaactgc cggcccaagc tgaccgccca gggcaatctg ccagcagaga
    24301 gtaaatacgg cgatcggcgg cataaaacgg atcgcataac gcgtcatctt gataacgcga
    24361 ttttcgacaa agaccggggc aaggcgtttt tccagcggcc acgtctttga gtaatgctgt
    24421 ccccggcgaa acaagctaaa aaaattaaca gaacgattat ccggcgttga catgcttcac
    24481 ctcaacttca catataaaga ttcaaaaatt tgtgcaaatt cacaactcag cgggacaaca
    24541 ttcaaaacat tttgtcttcc atacccacta tcaggtatcc tttagcagcc tgaaggccta
    24601 agtagtacat attcattgag tcgtcaaatt catatacatt atgccattgg ctgaaaatta
    24661 cgcaaaatgg catagactca agatatttct tccatcatgc aaaaaaaatt tgcagtgcat
    24721 gatgttaatc ataaatgtcg gtgtcatcat gcgctacgct ctatggctcc ctgacgtttt
    24781 tttagccacg tatcaattat aggtacttcc atgtcgagta agttagtact ggttctgaac
    24841 tgcggtagtt cttcactgaa atttgccatc atcgatgcag taaatggtga agagtacctt
    24901 tctggtttag ccgaatgttt ccacctgccc gaagcacgta tcaaatggaa aatggacggc
    24961 aataaacagg aagcggcttt aggtgcaggc gccgctcaca gcgaagcgct caactttatc
    25021 gttaatacta ttctggcaca aaaaccagaa ctgtctgcgc agctgactgc tatcggtcac
    25081 cgtatcgtac acggcggcga aaagtatacc agctccgtag tgatcgatga gtctgttatt
    25141 cagggtatca aagatgcagc ttcttttgca ccgctacaca acccggctca cctgatcggt
    25201 atcgaagaag ctctgaaatc tttcccacag ctgaaagaca aaaacgttgc tgtatttgac
    25261 accgcgttcc accagactat gccggaagag tcttacctct acgccctgcc ttacaacctg
    25321 tacaaagagc acggcatccg tcgttacggc gcgcacggca ccagccactt ctatgtaacc
    25381 caggaagcgg caaaaatgct gaacaaaccg gtagaagaac tgaacatcat cacctgccac
    25441 ctgggcaacg gtggttccgt ttctgctatc cgcaacggta aatgcgttga cacctctatg
    25501 ggcctgaccc cgctggaagg tctggtcatg ggtacccgtt ctggtgatat cgatccggcg
    25561 atcatcttcc acctgcacga caccctgggc atgagcgttg acgcaatcaa caaactgctg
    25621 accaaagagt ctggcctgct gggtctgacc gaagtgacca gcgactgccg ctatgttgaa
    25681 gacaactacg cgacgaaaga agacgcgaag cgcgcaatgg acgtttactg ccaccgcctg
    25741 gcgaaataca tcggtgccta cactgcgctg atggatggtc gtctggacgc tgttgtattc
    25801 actggtggta tcggtgaaaa tgccgcgatg gttcgtgaac tgtctctggg caaactgggc
    25861 gtgctgggct ttgaagttga tcatgaacgc aacctggctg cacgtttcgg caaatctggt
    25921 ttcatcaaca aagaaggtac ccgtcctgcg gtggttatcc caaccaacga agaactggtt
    25981 atcgcgcaag acgcgagccg cctgactgcc tgatttcaca ccgccagctc agctggcggt
    26041 gctgttttgt aacccgccaa atcggcggta acgaaagagg ataaaccgtg tcccgtatta
    26101 ttatgctgat ccctaccgga accagcgtcg gtctgaccag cgtcagcctt ggcgtgatcc
    26161 gtgcaatgga acgcaaaggc gttcgtctga gcgttttcaa acctatcgct cagccgcgta
    26221 ccggtggcga tgcgcccgat cagactacga ctatcgtgcg tgcgaactct tccaccacga
    26281 cggccgctga accgctgaaa atgagctacg ttgaaggtct gctttccagc aatcagaaag
    26341 atgtgctgat ggaagagatc gtcgcaaact accacgctaa caccaaagac gctgaagtcg
    26401 ttctggttga aggtctggtc ccgacacgta agcaccagtt tgcccagtct ctgaactacg
    26461 aaatcgctaa aacgctgaat gcggaaatcg tcttcgttat gtctcagggc actgacaccc
    26521 cggaacagct gaaagagcgt atcgaactga cccgcaacag cttcggcggt gccaaaaaca
    26581 ccaacatcac cggcgttatc gttaacaaac tgaacgcacc ggttgatgaa cagggtcgta
    26641 ctcgcccgga tctgtccgag attttcgacg actcttccaa agctaaagta aacaatgttg
    26701 atccggcgaa gctgcaagaa tccagcccgc tgccggttct cggcgctgtg ccgtggagct
    26761 ttgacctgat cgcgactcgt gcgatcgata tggctcgcca cctgaatgcg accatcatca
    26821 acgaaggcga catcaatact cgccgcgtta aatccgtcac tttctgcgca cgcagcattc
    26881 cgcacatgct ggagcacttc cgtgccggtt ctctgctggt gacttccgca gaccgtcctg
    26941 acgtgctggt cgccgcttgc ctggcagcca tgaacggcgt agaaatcggt gccctgctgc
    27001 tgactggcgg ctacgaaatg gacgcgcgca tttctaaact gtgcgaacgt gctttcgcta
    27061 ccggcctgcc ggtatttatg gtgaacacca acacctggca gacctctctg agcctgcaga
    27121 gcttcaacca ggaagttccg gttgacgatc acgaacgtat cgagaaagtt caggaatacg
    27181 ttgctaacta catcaacgct gactggatcg aatctctgac tgccacttct gagcgcagcc
    27241 gtcgtctgtc tccgcctgcg ttccgttatc agctgactga acttgcgcgc aaagcgggca
    27301 aacgtatcgt actgccggaa ggtgacgaac cgcgtaccgt taaagcagcc gctatctgtg
    27361 ctgaacgtgg tatcgcaact tgcgtactgc tgggtaatcc ggcagagatc aaccgtgttg
    27421 cagcgtctca gggtgtagaa ctgggtgcag ggattgaaat cgttgatcca gaagtggttc
    27481 gcgaaagcta tgttggtcgt ctggtcgaac tgcgtaagaa caaaggcatg accgaaaccg
    27541 ttgcccgcga acagctggaa gacaacgtgg tgctcggtac gctgatgctg gaacaggatg
    27601 aagttgatgg tctggtttcc ggtgctgttc acactaccgc aaacaccatc cgtccgccgc
    27661 tgcagctgat caaaactgca ccgggcagct ccctggtatc ttccgtgttc ttcatgctgc
    27721 tgccggaaca ggtttacgtt tacggtgact gtgcgatcaa cccggatccg accgctgaac
    27781 agctggcaga aatcgcgatt cagtccgctg attccgctgc ggccttcggt atcgaaccgc
    27841 gcgttgctat gctctcctac tccaccggta cttctggtgc aggtagcgac gtagaaaagg
    27901 ttcgcgaagc aactcgtctg gcgcaggaaa aacgtcctga cctgatgatc gacggtccgc
    27961 tgcagtacga cgctgcggta atggctgacg ttgcgaaatc caaagcgccg aactctccgg
    28021 ttgcaggtcg cgctaccgtg tttatcttcc cggatctgaa caccggtaac accacctaca
    28081 aagcggtaca gcgttctgcc gacctgatct ccatcgggcc gatgctgcag ggtatgcgca
    28141 agccggttaa cgacctgtcc cgtggcgcac tggttgacga tatcgtctac accatcgcgc
    28201 tgactgcgat tcagtctgca cagcagcagt aatctcgtca tcatccgcag ctttgcgctg
    28261 cggatatctg aaccggaaat aatcactatt tccggttttt tattctctta atctgcatta
    28321 atcctttctg attatcttgc ttaactgcgc tgcatcaatg aattgcgcca tttcactttg
    28381 catacttacc actttgtttt gtgcaaggga atatttgcgc tatgtccgca atcactgaat
    28441 ccaaaccaac aagaagatgg gcaatgcccg atacgttggt gattatcttt tttgttgcta
    28501 ttttaaccag ccttgccacc tgggtagttc cggtggggat gtttgacagt caggaagtgc
    28561 agtatcaggt tgatggtcaa acaaaaacac gcaaagtcgt agatccacac tcatttcgca
    28621 ttctgactaa cgaagcaggc gaacctgagt atcaccgcgt acagctgttc acgacgggcg
    28681 atgaacgccc gggcctgatg aacttcccgt ttgaaggatt aacctcagga tcgaaatacg
    28741 ggacagctgt tggcatcatc atgtttatgc tggtgattgg cggcgcgttt ggcattgtga
    28801 tgcgtacagg aaccattgat aacggtatcc tggcgcttat tcgccatacc cgcgggaatg
    28861 aaattctctt tattcctgcg ctgtttattc tgttttcact tggcggcgcg gtatttggta
    28921 tgggagaaga ggccgtcgcc tttgccatta tcatcgcacc gctaatggtc cggctgggct
    28981 atgacagtat taccaccgtc ctggtgacct atattgccac gcaaatcggt tttgccagtt
    29041 cgtggatgaa cccgttttgt gtggtcgttg ctcaggggat tgccggcgtt ccggtgcttt
    29101 ctggctccgg gttgcgcatc gtggtgtggg ttatcgccac tatgattggc ctgatcttta
    29161 ccatggtgta cgcctcacga gtgaaaaaga atcctcttct gtcacgcgtg catgagtccg
    29221 accgcttctt tcgtgaaaag caggcggatg ttgaacaacg tccgtttacc tttggtgact
    29281 ggctggtatt gattgtcctg accgccgtaa tggtctgggt gatttggggc gtgatcgtta
    29341 atgcctggtt tattccagaa attgccagcc agttcttcac catgggtctg gtgattggca
    29401 tcatcggcgt tgtttcccgc cttaacggca tgacggttaa taccatggct tcatccttta
    29461 ccgaaggggc gcgaatgatg atcgcccctg ccctgctggt gggtttcgcc aaagggattt
    29521 tgctgctggt cggtaatggt gaagcgggtg atgccagcgt gttaaatacc atcctcaaca
    29581 gcattgccaa tgccattagc ggtctggaca acgcggtcgc ggcctggttt atgttgctct
    29641 tccaggcggt atttaatttc ttcgtgacgt ccggttctgg tcaggcggcg ttaaccatgc
    29701 cgttactggc accgcttggc gatctggtcg gtgttaaccg tcaggttacc gtgctggcat
    29761 tccagtttgg tgatggcttc agtcacatca tttacccaac ctcagcttcg ttaatggcaa
    29821 ctctcggtgt gtgcagggtg gacttccgta actggctgaa ggtgggcgcg accctacttg
    29881 gactgctgtt tattatgtcc agcgtcgtag tgatcggcgc tcagttgatg ggctaccact
    29941 aaaaatgtta agagccgcaa ttgcggctct tttttcattc tgccgtttca gtctctacgg
    30001 cttcattttt ggcattgcgc ttcatccaca acgccagcgc tttcagcgag tctggagtga
    30061 actcatcgca gcgtgcggtg atttcttccg gcgtcagcca gcaaacttca ctgacttcat
    30121 cttcctgtag ggcgaaggga ccgtgagaga cgcagctgaa caacgcgccc cagacacggc
    30181 aatttttatc ttcgaaatag aactgcccgt gctcggcaaa ggggacaccg gcaatgccca
    30241 actcttcttc cgcttcgcga cgcgcggatt ccagcagttg ctcatcggcc tgaaccacac
    30301 cgcctgcggt cgcatctaac atgccgggta aaaagtcttt tgtctcggta cgacgctgga
    30361 ccagaatttt gcccatgcca tcatgcacga cgatgtaagt tgcacgatga cgcagacact
    30421 gtgcccgcat ttgttcccgg ctggcttgtg caatgacttc gttctcttca ttgacaatat
    30481 ccacccattc agtacttgcc aaacgacgct gttccattat caggaaacct tctttttctg
    30541 gcgctcttac ggcgcatttt gagttgtggg taaattacgg attaatcgcg acctgcgcaa
    30601 tgatactttg atcattgagt gcgattacac taagtacgtc attatccagc atgccataac
    30661 tcgccggatt accgcctttc ggaatactca ccgagccggg gttgaagtgg aaaatctccc
    30721 cccgctgttc tgccactggc agatgggtat gaccgtacac cagcacatcg ttctggttta
    30781 aagcaggtag attttccggg ccaaaaagat gaccatgcgt caaaaacaga cgttgttttt
    30841 ccagtaatac ctgttgccac ggcgcggtta tcgggaaatg cagcagcatt tgatccactt
    30901 cgctgtcgca gttgccgcgc acagcgataa ccttatgtgc cacttcatta agccgttcag
    30961 cgactttggc tggcgcgtaa ccctccggta aagcattacg cgggccgtga ttcaacacgt
    31021 cgccaaggat caccagccac tgggcaccgc tttgggcaaa caactccaga acacgttccg
    31081 tcgccggtaa cgacccatga atgtccgatg caaacatcag tttcatcact cactcctcgt
    31141 cgaaaaaacg taagctatga tactggattc tgtcgttgct atcagccaga ttgcttcgcc
    31201 gaaagtgcaa taaaacgctg gacagacgct cgctgccact ggaatgtcgc ataatccacc
    31261 aggcgttctg gcacctcatc gccatgtagt accaggcggt taatcattag cgccagatca
    31321 gtatcagcaa tgcaccattc accaaataaa ttcggctgac ccagtgctaa caaatgttct
    31381 gccatcgcga cactggcttt tccctcggcc gttagtggcg cttttttcgc ccccgcaaag
    31441 acaacatccg tcggacgctc ttcgcggatg ggcatcagat cgctgcgcag ccaggcctga
    31501 atctgtcgtg cacgcgcacg attttctaaa tcaagcggat aaatacgttc ccaggtcggt
    31561 ggcgcaaatc gatcttccag atactccgca atggcagaag attcactcaa ctcaaaatca
    31621 tcgatttgta ataacggcac acggcgtgtc tgaccgtaac cttgccacgt cggctgcaaa
    31681 tgttcaccgc tgtcgaggtc gatggtcttg atatgaaacg acaggccttt ttcctgcaac
    31741 gccacccaag cggataacac ataaggggag aaaaagtggg catctgacca aagcgtgata
    31801 gcgggtttac tcattatgtc ctcggcaata aaggaggctg gctatcaaac tatagagcct
    31861 ttttgtcact gtcacgtgac gaatactcac gtaagtcgcc gcgctttcta tacttgtggt
    31921 gttcagatga ttaaacaacc ggagctgcaa tgatcgatct ctatttcgcc ccgacaccca
    31981 atggtcacaa aattacgctg tttctcgaag aagcagagct tgattatcgc ttgattaagg
    32041 tagacctggg gaaaggcggt cagtttcgtc cggaattttt gcgcatttcg cccaacaaca
    32101 aaattccggc aattgttgat cattctcctg ccgatggcgg cgaaccgcta agcctctttg
    32161 aatctggtgc cattttgttg tatctggctg agaaaacggg gctctttttg agtcatgaaa
    32221 cgcgtgaacg cgccgccaca ttacagtggt tattctggca ggtaggcgaa ctggggccga
    32281 tgcttgggca aaatcatcat tttaatcacg cagcccccca aaccattcct tacgctattg
    32341 aacgttatca ggttgaaact cagcgtcttt accatgtact aaacaagcgg ctggaaaact
    32401 cgccctggct gggaggcgag aactacagca ttgcggatat tgcctgctgg ccgtgggtta
    32461 atgcctggac tcgccagcga attgacctcg caatgtatcc ggcagtcaag aactggcatg
    32521 agcggatccg ttcgcgccct gccaccgggc aggcactgct aaaagcacaa ctcggtgatg
    32581 agcgttcgga tagttaacag aaacaggttc tcgtgtatta tttcatccta agtaaaacaa
    32641 cggagaacct gcaatggcac aacctgccgc tattattcgt ataaagaacc ttcgtttacg
    32701 tacgtttatc ggaattaagg aagaagaaat taacaaccgt caggatattg ttatcaatgt
    32761 gacgatccac taccccgccg ataaagcgcg cactagcgaa gatatcaacg atgcgctgaa
    32821 ttatcgcacc gtaacgaaaa acattattca gcatgtagag aataaccgtt tctctttgtt
    32881 ggaaaaatta actcaggatg tgctcgatat cgcacgtgaa catcactggg tgacgtatgc
    32941 tgaagtggag atcgataaac tgcacgcgct gcgctacgcc gattcggtat ccatgacctt
    33001 aagctggcag cgttaaccgc catatcggga ggctgcatga acatagtgat caccggaggg
    33061 acgggattaa ttggtcgcca tttgattccg cgtttgctgg agctgggcca tcaaatcacg
    33121 gtagtgacgc gtaacccaca gaaagccagt tccgttctcg gccctcgggt gacgctatgg
    33181 caagggctag ccgatcaaag caacctcaac ggcgttgatg cggtaatcaa cctggccgga
    33241 gaaccgattg ctgataaacg ctggactcac gagcaaaaag agcgtctctg ccaaagccgc
    33301 tggaatatca cgcaaaaact ggtcgatttg attaatgcca gcgacacgcc gccgtcggta
    33361 ctcatttccg gctcggcaac gggctattat ggcgacttag gtgaagtggt ggttaccgaa
    33421 gaggaaccgc cgcataacga atttactcat aaactctgcg cccgctggga agagattgcc
    33481 tgccgggcgc aaagtgacaa aacgcgagtg tgcctgctgc gaaccggcgt ggtactggca
    33541 ccggacggcg gtattctcgg taaaatgctg ccaccgtttc gtcttggcct gggcgggccg
    33601 attgactctg gtcggcagta tctggcctgg attcatattg atgatatggt caacggtatt
    33661 ctctggctgc tggataacga gctgcgcggg ccatttaata tggtttcgcc ctacccggta
    33721 cgcaatgaac aatttgccca tgcgctcggt cacgcgctgc atcgcccggc cattttgcgc
    33781 gtccccgcga ccgccattcg actgttaatg ggcgaatctt cagtactggt attaggtgga
    33841 caacgcgcgc tgcctaaacg gctggaagaa gcgggttttg cgtttcgctg gtacgattta
    33901 gaagaggcgc tggcggatgt cgttcgctga tgtggtttac agcaaacatc cgccagttaa
    33961 ctcccggtgt tacaggatta gtggctttgc gcgataagat cgtctggtga aagtcgggtc
    34021 accatcataa ctaactctct gtctaaacct ctatccagca tctcctgagc aatacgcagg
    34081 gcttcttcgt gtttgccctg cattgcgcct tcttcacgta atctgtcagc aatggtcatc
    34141 agtttctcct tttcttgtgg tgcgcgttcc gctatctcac caataaatgc acgaaaacgc
    34201 tgggcatccc ctgtttgtaa tacgtaatta aacagggctt ttagctgtct gtcattagtg
    34261 ttccctgtaa ctagcagcga aacaatttgg tcgactaatc ccaacagatc gcgctgacga
    34321 atatgtttct gaattaactc caacagcgcc attttgcggt gttgcataat ctcgtcatcc
    34381 ggcaccacgg taatatccac caacggaaac gctgatgaat atattttgcg ggctatagca
    34441 ggctcggcaa attcatcaag ccagcagagt gaataaggat aaggacttct gcaaccatga
    34501 taaaacagca tcgggatcac caatggaagc tctttatagc ccg
//
LOCUS       DAJTMC010000025        34160 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.025, whole genome shotgun sequence.
ACCESSION   DAJTMC010000025 DAJTMC010000000
VERSION     DAJTMC010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34160
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.025"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(312..524)
                     /gene="rpmE"
                     /locus_tag="OO130_001058"
     CDS             complement(312..524)
                     /gene="rpmE"
                     /locus_tag="OO130_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HCR5364551.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            727..2925
                     /gene="priA"
                     /locus_tag="OO130_001059"
     CDS             727..2925
                     /gene="priA"
                     /locus_tag="OO130_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HCR5364552.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     VVVSVSDVSELPLNELKAVVEVLDVEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPMFPGVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWILGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            3081..4106
                     /gene="cytR"
                     /locus_tag="OO130_001060"
     CDS             3081..4106
                     /gene="cytR"
                     /locus_tag="OO130_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HCR5364553.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            4198..5157
                     /gene="ftsN"
                     /locus_tag="OO130_001061"
     CDS             4198..5157
                     /gene="ftsN"
                     /locus_tag="OO130_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HCR5364554.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHNKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            5250..5780
                     /gene="hslV"
                     /locus_tag="OO130_001062"
     CDS             5250..5780
                     /gene="hslV"
                     /locus_tag="OO130_001062"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HCR5364555.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            5790..7121
                     /gene="hslU"
                     /locus_tag="OO130_001063"
     CDS             5790..7121
                     /gene="hslU"
                     /locus_tag="OO130_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HCR5364556.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            7188..8114
                     /gene="menA"
                     /locus_tag="OO130_001064"
     CDS             7188..8114
                     /gene="menA"
                     /locus_tag="OO130_001064"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HCR5364557.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDRPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFILGIFLSQWAA"
     gene            8207..8692
                     /gene="rraA"
                     /locus_tag="OO130_001065"
     CDS             8207..8692
                     /gene="rraA"
                     /locus_tag="OO130_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HCR5364558.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(8777..9022)
                     /gene="zapB"
                     /locus_tag="OO130_001066"
     CDS             complement(8777..9022)
                     /gene="zapB"
                     /locus_tag="OO130_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HCR5364559.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            9448..10293
                     /gene="glpF"
                     /locus_tag="OO130_001067"
     CDS             9448..10293
                     /gene="glpF"
                     /locus_tag="OO130_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HCR5364560.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVKEKETTTPSE
                     QKASL"
     gene            10316..11824
                     /gene="glpK"
                     /locus_tag="OO130_001068"
     CDS             10316..11824
                     /gene="glpK"
                     /locus_tag="OO130_001068"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HCR5364561.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRSE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            11959..12969
                     /gene="glpX"
                     /locus_tag="OO130_001069"
     CDS             11959..12969
                     /gene="glpX"
                     /locus_tag="OO130_001069"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HCR5364562.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            13066..13812
                     /gene="fpr"
                     /locus_tag="OO130_001070"
     CDS             13066..13812
                     /gene="fpr"
                     /locus_tag="OO130_001070"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HCR5364563.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(13817..14245)
                     /gene="uspD"
                     /locus_tag="OO130_001071"
     CDS             complement(13817..14245)
                     /gene="uspD"
                     /locus_tag="OO130_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HCR5364564.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(14272..14571)
                     /locus_tag="OO130_001072"
     CDS             complement(14272..14571)
                     /locus_tag="OO130_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HCR5364565.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSVDEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(14783..15223)
                     /locus_tag="OO130_001073"
     CDS             complement(14783..15223)
                     /locus_tag="OO130_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCR5364566.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     CFVPTLILVMMLIDLGAFVGTQGENKYGKDTQDVKYKADNKSSN"
     gene            15324..15923
                     /locus_tag="OO130_001074"
     CDS             15324..15923
                     /locus_tag="OO130_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HCR5364567.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            16031..16798
                     /gene="tpiA"
                     /locus_tag="OO130_001075"
     CDS             16031..16798
                     /gene="tpiA"
                     /locus_tag="OO130_001075"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HCR5364568.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(16853..17608)
                     /gene="cdh"
                     /locus_tag="OO130_001076"
     CDS             complement(16853..17608)
                     /gene="cdh"
                     /locus_tag="OO130_001076"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HCR5364569.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(17715..18704)
                     /gene="sbp"
                     /locus_tag="OO130_001077"
     CDS             complement(17715..18704)
                     /gene="sbp"
                     /locus_tag="OO130_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HCR5364570.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVMRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(19024..19986)
                     /gene="pfkA"
                     /locus_tag="OO130_001078"
     CDS             complement(19024..19986)
                     /gene="pfkA"
                     /locus_tag="OO130_001078"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HCR5364571.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(20167..21069)
                     /gene="fieF"
                     /locus_tag="OO130_001079"
     CDS             complement(20167..21069)
                     /gene="fieF"
                     /locus_tag="OO130_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312867.1"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HCR5364572.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLISPTPMIDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(21217..21729)
                     /gene="cpxP"
                     /locus_tag="OO130_001080"
     CDS             complement(21217..21729)
                     /gene="cpxP"
                     /locus_tag="OO130_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HCR5364573.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQRS
                     TQSHMFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQ
                     AEKMANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLK
                     LLSSSNSRSQ"
     gene            21879..22577
                     /gene="cpxR"
                     /locus_tag="OO130_001081"
     CDS             21879..22577
                     /gene="cpxR"
                     /locus_tag="OO130_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HCR5364574.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            22574..23947
                     /gene="cpxA"
                     /locus_tag="OO130_001082"
     CDS             22574..23947
                     /gene="cpxA"
                     /locus_tag="OO130_001082"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HCR5364575.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(24053..24727)
                     /gene="yiiM"
                     /locus_tag="OO130_001083"
     CDS             complement(24053..24727)
                     /gene="yiiM"
                     /locus_tag="OO130_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HCR5364576.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWAREFPEQAELFVAPAFGENLSTDGLTESNVYMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(24876..25841)
                     /gene="kdgT"
                     /locus_tag="OO130_001084"
     CDS             complement(24876..25841)
                     /gene="kdgT"
                     /locus_tag="OO130_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HCR5364577.1"
                     /translation="MRKSRGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMITGTVSI
                     LAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVEVGFFAG
                     LSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGTAGIASF
                     EPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTVIAQTGL
                     LGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPMLIAEMVPAFKP
                     MAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(26120..26740)
                     /gene="sodA"
                     /locus_tag="OO130_001085"
     CDS             complement(26120..26740)
                     /gene="sodA"
                     /locus_tag="OO130_001085"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HCR5364578.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            27024..28058
                     /gene="rhaT"
                     /locus_tag="OO130_001086"
     CDS             27024..28058
                     /gene="rhaT"
                     /locus_tag="OO130_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HCR5364579.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWAISALLLPNFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGMSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLLNTEGGRMTLLGVLVALIGVGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     IIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKPLIIHNVLLSALGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWNNAGRRPVTVLSLGCVVIIVA
                     ANIVGIGMAN"
     gene            complement(28055..28921)
                     /gene="rhaR"
                     /locus_tag="OO130_001087"
     CDS             complement(28055..28921)
                     /gene="rhaR"
                     /locus_tag="OO130_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HCR5364580.1"
                     /translation="MAEVATVAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDF
                     CELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKL
                     NLDWQGAIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVSFANEMAELLFGQ
                     LVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQ
                     FRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMT
                     PSQWRHLNSQKD"
     gene            complement(28977..29813)
                     /gene="rhaS"
                     /locus_tag="OO130_001088"
     CDS             complement(28977..29813)
                     /gene="rhaS"
                     /locus_tag="OO130_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HCR5364581.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            30101..31513
                     /gene="rhaB"
                     /locus_tag="OO130_001089"
     CDS             30101..31513
                     /gene="rhaB"
                     /locus_tag="OO130_001089"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HCR5364582.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWNVDSLESAIRLGLNKVCEEGIRIDSIGLDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRSNGLMAQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPEAMCSEIQAACRETAQPIPESDAELAQLRGEDFSQLHIVGGGCQNTLLNQ
                     LCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDFRQVVSTTANLTTFTPNPDS
                     EIAHYVAQLHSTRQTKELCA"
     gene            31510..32769
                     /gene="rhaA"
                     /locus_tag="OO130_001090"
     CDS             31510..32769
                     /gene="rhaA"
                     /locus_tag="OO130_001090"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HCR5364583.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWGEWAKANQLGLDFNPSCFSHPLSADGFTLSHPDDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISKKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDCDHVVLLDDETQAIASEIVRHDLFDQVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKEILSQRG"
     gene            33036..33860
                     /gene="rhaD"
                     /locus_tag="OO130_001091"
     CDS             33036..33860
                     /gene="rhaD"
                     /locus_tag="OO130_001091"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HCR5364584.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAP
                     YKDNVHAQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     STLDETFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGKRFGVTPLASALAL"
     gene            33870..>34160
                     /gene="rhaM"
                     /locus_tag="OO130_001092"
     CDS             33870..>34160
                     /gene="rhaM"
                     /locus_tag="OO130_001092"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HCR5364585.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFATVEIESEERWNAVASADVCQRWWKYMTDVMPANPDNSPVSSEL"
BASE COUNT         8370 a         8914 c         8867 g         8009 t
ORIGIN      
        1 ggattgaccg gatggccgga cgtcaccccg ccggggcaca ctgaacaggc cacgccggtg
       61 ggagcaccaa tgcgcacccc ggctgaaccc tgaacaatgc taccgccata ctgcgtcatg
      121 tcgccctgac gcgccgccgg ttttccgctc atcgtaaaat ccttttgaaa taaataagaa
      181 ccagaagcct agcgccagtc aacaacagga aatgataatg agttaacacc ccggaatttt
      241 tcagaattta taaaaggcaa aattcaggca caaaaaaagc gccgtgcggc gcttttttcg
      301 gaaatccggt cttatttgct gcccgggatg ttgaaacgct tgttgaagcg gtcaacacgg
      361 ccaccggtag caacatcacg ctgtttgcca gtgaagaacg ggtggcactt gctgcacacg
      421 tcgaggttca ggtcatgacc aacagtggag cggattttca ttacgttacc gcaagagcag
      481 ctagcagtaa tttcttcgta tttcgggtga atatcttttt tcatgggaaa acctcggttt
      541 aaggccgcgt cgctcttcca gccctaacgc cagacaccac gcgatgttaa aagtatagct
      601 tcaatacgat catttcgtac gaaggcgcga aatcatacag aaattaacca acgtatgcaa
      661 actgatccgc actcttctac ggcaatgtgt atactaaccc accgaatttc aagtcaggat
      721 gatgctatgc ccgttgccca cgttgccttg cccgttccgc ttcctcgtac ctttgactat
      781 ctgctgccag aaggcatgac ggttaaagcc gggtgtcgcg tgcgcgtgcc gtttggcaaa
      841 cagcaggagc gcatcggggt tgtggtgtca gttagcgatg tcagtgagct gccgcttaac
      901 gagctgaaag cggtagtgga agtgctggac gttgagccgg tatttactca ctccgtctgg
      961 cggttactgc tctgggcggc agattactat catcatccga ttggcgatgt gctgtttcat
     1021 gccttgccga ttttactgcg ccaggggcgg cctgcggcga acgcgccgat gtggtactgg
     1081 tttgccactg aacaaggcca ggcggtggat ctgaacagcc tgaaacgctc ccccaagcaa
     1141 caacaggcgc tggcggcgtt acggcaaggc aaaatctggc gcgaccaggt cgccacgctc
     1201 gaatttaatg atgccgcgtt gcaggcgcta cgcaaaaaag gtctgtgtga tttagcaagt
     1261 gaaacaccag agtttagcga ctggcgaacg aactatgccg tttctggtga gcggttgcga
     1321 ttgaataccg aacaggccac cgccgttggc gcaattcata gcgcggcaga tactttttct
     1381 gcctggctgc tggcgggcgt taccggttcc ggtaaaacgg aggtttatct cagtgtactg
     1441 gaaaacgtgc tcgctcaggg caaacaggcg ctggtgatgg tgccggaaat cggcctgaca
     1501 ccgcaaacta tcgcccgttt tcgtgaacgt tttaatgccc ccgtggaagt tctgcattcc
     1561 ggcctgaacg acagcgagcg gctttctgcg tggttgaaag cgaaaaatgg cgaggcggcg
     1621 attgtgatcg gcacccgctc cgcgctgttt acgccgttta aaaatctcgg cgtgattgtc
     1681 attgatgaag agcacgacag ctcctacaag cagcaggaag gctggcgcta tcatgcccgc
     1741 gacctggcgg tgtatcgtgc gcacagcgag caaatcccga ttattcttgg ctccgcaacg
     1801 cccgcgctgg aaacgttatg caacgtccag cagaaaaaat accgcctgct gcgcctgacc
     1861 cgtcgggcag ggaatgctcg tccggcaatt caacatgtgc tggatttaaa aggtcagaag
     1921 gtgcaggcag gtctggctcc ggcgttaatc acccgtatgc gccagcattt acaggccaac
     1981 aatcaggtta tcctctttct taatcgtcgg ggttttgcgc ctgcgctgct gtgccacgac
     2041 tgcggctgga tagccgaatg cccacgttgc gatcactact acacgctgca tcaggcgcaa
     2101 cagcatttgc gctgtcacca ctgtgacagt cagcgtccgg tgccgcgcca gtgcccttcc
     2161 tgcggttcca cgcacctggt ccccgtgggg ctgggcaccg aacagcttga acagacgctt
     2221 gcgccgatgt tccccggcgt gcccatttca cgtatcgacc gcgataccac cagccgcaaa
     2281 ggggcactgg aacagcaact ggcggaagtg catcgcggcg gcgcgaggat tttgatcggc
     2341 acacaaatgc tggcgaaagg tcaccacttt ccggatgtga cgctggtggc gttactggat
     2401 gtggacggcg cgctgttttc tgccgatttt cgctcggcag aacgtttcgc ccagctttac
     2461 acccaggtcg ccggtcgtgc cgggcgtgcg ggtaaacagg gcgaagtggt gctgcaaacg
     2521 caccatccgg aacatcctct gttgcaaacg ttgctctata aaggctacga cgcctttgcc
     2581 gaacaggcgc tggctgagcg acgcatgatg cagctgccgc cgtggaccag ccatgtgatt
     2641 gtgcgtgcgg aagatcataa caatcagcac gcgccgctgt tcctgcaaca actgcgtaat
     2701 ctgatccttt ccagcccgct ggcagacgac aaactgtgga ttctcggccc ggttccggct
     2761 ctggcaccta agcgtggtgg ccgctggcgc tggcagatct tgttgcagca cccttcccgc
     2821 gtgcgcttgc aacacatcat tagtggtacg ctggcgctta ttaatacaat accggattcc
     2881 cgcaaggtga aatgggtgtt ggatgtcgat cccatcgaag gctaatgcgc aaacgttgcg
     2941 aggcggatcg aaaaattcaa tattcatcac acttttcatg aaaattctgt aaccgttttc
     3001 acgcgctatc tgctaaaaat gttgccgatg tgaagtaaac atggatgtag tacgcctgac
     3061 gtaccaggcg aggagtgagt gtgaaagcga agaagcagga aactgccgcg accatgaaag
     3121 acgttgccct caaggcaaaa gtctctacag cgactgtctc ccgagcatta atgaatcccg
     3181 ataaagtctc ccaggccacc cgtaatcggg ttgaaaaagc ggcccgggaa gtgggttatt
     3241 taccgcagcc tatggggcgc aacgtcaagc gtaatgaatc ccgcaccatt ctggtgattg
     3301 tcccggatat ctgcgatccc ttctttagcg aaattattcg cggtatcgaa gttacggcgg
     3361 caaatcacgg atatctggtg ctgattggcg actgtgcgca tcaaaatcag caggaaaaaa
     3421 cctttatcga tttgatcatc accaagcaaa ttgatggcat gttgctgctg ggttcaaggc
     3481 tgccgtttga tgccagcatt gaggaacagc gtaatctgcc gccgatggtg atggcgaacg
     3541 aatttgcacc ggaactggag ctgcctaccg ttcatatcga caatctgacc gccgcatttg
     3601 atgcagtaaa ttatttatat gagcaagggc ataaacggat tggctgtata gccggtcccg
     3661 aagagatgcc actgtgtcac taccgcctgc aaggctatgt tcaggcgctg cgtcgctgcg
     3721 gaattatggt tgatccacaa tacatcgccc gtggcgactt caccttcgaa gccggaagca
     3781 aagcaatgca acagctgctt gatcttccac aaccgcctac tgcagtcttc tgccatagcg
     3841 atgtgatggc gctcggcgca ctttctcagg caaaacgcca ggggctgaaa gtcccggaag
     3901 acctttccat aatcggtttt gataacatcg acctgacgca attttgtgat ccgccgctga
     3961 caaccatcgc gcagccgcgt tacgaaatcg gtcgggaagc tatgctgtta ttgcttgatc
     4021 aaatgcaggg gcaacacgtt ggcagtggct ctcgtttaat ggactgcgaa cttatcatcc
     4081 ggggatcaac acgcgcgtta ccttaaagta aacctttaag actgccgtga ctggtcaaag
     4141 gcccgccgct taagtaacat ggcgggctga cgaacgaata aatacagcga aacgatagtg
     4201 gcacaacgag attatgtacg ccgcagccaa ccggcacctt cgcggcgaaa aaagagcacc
     4261 tcacggaaaa agcaacgaaa tctgcctgcg gtttctcccg ctatggtcgc tattgctgcc
     4321 gccgttcttg tgacctttat cggtggtctg tacttcatta cgcatcacaa taaagaagag
     4381 tccgagacgc tgcaaagcca gaaagtgacc ggaaacggac taccaccaaa accagaagaa
     4441 cgctggcgct acattaaaga gctggaaagt cgccagccgg gtgtgcgtgc gcccacagaa
     4501 ccttctgccg gtggtgaagt gaaaacgccg gagcaactga caccagaaca acgtcagctt
     4561 cttgaacaaa tgcaggctga tatgcgccag cagccaacgc agctggttga ggttccgtgg
     4621 aatgaacaga cgccggaaca gcgtcagcaa acgctacaac gccagcgtca ggcgcagcaa
     4681 ttagcggagc agcaacgcct ggctcagcag tcgcgtacga ctgaacaaag ctggcagcag
     4741 cagacgcgta cgtcgcaagc cgcgccggta caggcgcaac cgcgccagtc taaaccagct
     4801 tctactcagc agccgtacca ggatctgctg caaacccctg cgcacacgac cgcgcagtcg
     4861 aagccgcagc aagctgcacc ggttgctcgt gccgctgacg cgccaaaacc gacggcggag
     4921 aaaaaagacg aacgccgctg gatggtgcag tgcggttcgt tcagaggcgc ggaacaggca
     4981 gagacggtac gtgctcagct ggcgttcgaa ggatttgact cgaaaatcac caccaacaat
     5041 ggctggaatc gtgtggtcat tggcccggtg aaaggcaaag agaacgcaga cagcaccctc
     5101 aatcggttga agatggcggg tcatacaaac tgcattcggc tcgccgccgg gggttgaaac
     5161 cctcaaaatc ccccccatct ataattgcat tatgcctcgt acttttgtac ggggtttgta
     5221 ctctgtattc gtaaccaagg ggtcagctcg tgacaactat agtaagcgta cgccgtaacg
     5281 gccatgtggt catcgctggt gatggtcagg ccacgttggg caataccgta atgaaaggca
     5341 acgtgaaaaa ggtccgccgt ctgtacaacg acaaagtcat cgcgggcttt gcgggcggta
     5401 ctgcggatgc ttttacgctg ttcgaactgt ttgaacgtaa actggaaatg catcagggcc
     5461 atctggttaa agccgccgtt gagctggcaa aagactggcg taccgatcgc atgctgcgca
     5521 aacttgaagc actgctggca gtcgcggatg aaactgcatc gcttatcatc accggtaacg
     5581 gtgacgtggt gcagccagaa aacgatctta ttgctatcgg ctccggcggc ccttacgccc
     5641 aggctgcggc gcgcgcgctg ttagaaaaca ctgaacttag cgcccgtgaa attgctgaaa
     5701 aggcgttgga tattgcaggc gacatttgca tctataccaa ccatttccac accatcgaag
     5761 aattaagcta caaagcgtaa ggatctccca tgtctgaaat gaccccacgc gaaatcgtca
     5821 gcgaactgga taagcacatc atcggccagg acaacgccaa gcgttctgtg gcgattgctc
     5881 tgcgtaaccg ctggcgtcgc atgcagctca acgaagagct gcgccatgaa gtgaccccga
     5941 aaaatatcct gatgatcggc ccgaccggtg tcggtaaaac tgaaatcgcc cgtcgtctgg
     6001 ctaagctggc gaatgcgccg ttcatcaaag ttgaagcgac caaattcacc gaagtgggct
     6061 atgtcggtaa ggaagtggat tctattattc gcgatctgac cgatgccgcc gtgaaaatgg
     6121 tacgcgtcca ggctatcgag aaaaaccgtt atcgcgctga agaactggca gaagaacgta
     6181 ttctcgacgt gctgatccca cctgctaaaa acaactgggg acagaccgaa cagcagcagg
     6241 aaccgtccgc tgctcgtcag gcattccgca aaaaactgcg tgaaggccag cttgatgaca
     6301 aagaaatcga gatcgatctt gccgcagcac cgatgggcgt tgaaattatg gctcctccag
     6361 gcatggaaga gatgaccagc cagctgcagt ccatgttcca gaacctgggc ggccagaagc
     6421 aaaaagcgcg taagctgaaa atcaaagacg ccatgaagct gctgattgaa gaagaagcgg
     6481 cgaaactggt gaacccggaa gagctgaagc aagacgctat cgacgctgtt gagcagcacg
     6541 ggatcgtgtt tatcgacgaa atcgacaaaa tctgtaagcg cggcgagtct tccggtccgg
     6601 atgtttctcg tgaaggcgtt cagcgtgacc tgctgccgct ggtagaaggt tgcaccgttt
     6661 ccaccaaaca cgggatggtc aaaactgacc acattctgtt tatcgcttct ggcgcgttcc
     6721 agattgcgaa accgtctgac ctaatcccgg aactgcaagg tcgtctgcca atccgcgttg
     6781 aactgcaggc actgaccacc agcgacttcg agcgtattct gaccgagccg aatgcctcta
     6841 tcaccgtgca gtacaaagca ctgatggcga ctgaaggcgt aaatatcgag tttaccgact
     6901 ccggtattaa acgcatcgcg gaagcggcat ggcaggtgaa cgaatctacc gaaaacatcg
     6961 gtgctcgtcg tttacacact gttctggagc gtttaatgga agagatttcc tacgacgcca
     7021 gcgatttaag cggtcaaaat atcactattg acgcagatta tgtgagcaaa catctggatg
     7081 cgttggtggc agatgaagat ctgagccgtt ttatcctata atcgcgttca atcattttca
     7141 tcattgtttg atggggctga aaggccccat ttttattggc gcgtattatg actgaacaac
     7201 aaattagccg aactcaggcg tggctggaaa gtttacgacc taaaaccctc cccctcgcct
     7261 ttgctgcaat tatcgtcggg acggcgctgg catggtggca aggtcatttt gatccgctgg
     7321 tcgccctgct ggcactgatt accgccgggc tattacagat cctttctaac ctcgccaatg
     7381 attacggcga tgcggtaaaa ggcagcgata gacctgaccg cattgggccg ctacgcggca
     7441 tgcaaaaagg ggtcattacc cagcaagaga tgaaacgggc gctcattatt accgtcgtgc
     7501 tcatctgtct ctccgggctg gcactggttg cagtggcgtg ccatacgctg gctgattttg
     7561 tcggtttcct gattcttggc gggttgtcga tcattgccgc tatcacctac accgtgggca
     7621 atcgtcctta tggttatatc ggtctggggg atatttccgt actggttttc tttggctggt
     7681 taagcgtcat ggggagctgg tatttacagg ctcatacatt gattccggca ctgatccttc
     7741 cggcgaccgc atgcggcctg ctggcaacgg cagtactgaa cattaataac ctgcgtgata
     7801 tcaatagcga ccgagaaaac ggcaaaaaca cgctggtggt gcgcttaggt gaagtgaacg
     7861 cgcgtcgtta tcatgcctgc ctgctgatgg gctctctggt atgtctggcg ctgtttaatc
     7921 tcttttcgct gcatagcctg tggggctggc tgttcctgct ggcggcaccg ttgctggtga
     7981 agcaagcccg ttatgtgatg cgggaaatgg acccggtggc gatgcgaccg atgctggaac
     8041 gcactgtcaa gggggcgtta ctgactaacc tgctgtttat tttagggata ttcctaagcc
     8101 agtgggcagc ataactgaca aatatcaatt aacaattgat gattttgcca acagcccaca
     8161 tagcgcgata tactgaaaat tctcgcagca actgaatgtt aagcctatga aatacgatac
     8221 ttccgagctt tgtgacatct atcaagaaga tgttaacgtc gtggaaccgc tgttctccaa
     8281 ctttggcgga cgggcgtcgt ttggcggaca aataatcacg gtaaaatgtt tcgaggacaa
     8341 cgggttgctg tacgatctgc tcgaacagaa tggccgtggt cgtgttcttg tcgtcgatgg
     8401 cggtggttct gttcgtcgcg cactggtcga tgctgaactg gcgcgtctgg cagtacaaaa
     8461 tgaatgggaa ggtctggtta tttacggcgc ggtgcgtcag gtagatgacc tggaagagtt
     8521 ggatatcggc atccaggcga tggcggcaat tccggttggt gccgctggcg aaggcattgg
     8581 cgaaagcgat gtccgcgtca attttggcgg tgtaaccttc ttctccggcg accatcttta
     8641 tgccgacaat accgggatta ttctttcaga agatccgctg gatattgaat gataagaaag
     8701 gcaccgcaag gtgccttttt tctgcgttac ctgttggcct acacagtaaa gaaattacgc
     8761 gaaagatgaa gcgtaatcag acctcttcca tgcgacccag cagggcctgc agacgttcct
     8821 gccagccgtt ctgctgttct ttcagatggt tgttctcacg ctccagctct tcgcgctgat
     8881 gctgggcatt ttgaacttcc tgcgacagtg agttgttttt ttctttcagc tcttcgattt
     8941 ccatctgcaa cagagtgatg gtatcaatcg cctgctgtac ttttgcttcc agtttctcaa
     9001 acacttctaa tgacattgtc atacctctcc tgaattgcaa ggcgttgatg gataaaaaat
     9061 cctcgtcccg attaccggtg acgccttaat aaatacgagc gcactttagt tagctccgat
     9121 tgtatgaagc cgcgccatcg ctgtccagcg gcacgccttg cagattacgg tttgccacac
     9181 ttttcatcct tctcctggtg acataatcca catcaatcga aaatgttaat aaatttgttg
     9241 cgcgaatgat ctaacaaaca tgcatcatgt acaatcagat ggaataaatg gcgcgataac
     9301 gctcatttta tgacgaagca cacacatttt aagttcgata tttctcgttt ttgctcgtta
     9361 acgataagtt tacagcatgc ctacaagcat cgtggaggtc cgtgactttc acgcatacaa
     9421 caaacattaa ctcttcagga tccgattatg agtcaaacat caaccttgaa aggccagtgc
     9481 attgctgaat tcctcggtac cgggttgttg atttttttcg gtgtgggttg cgttgcagca
     9541 ctaaaagtcg ctggtgcgtc ttttggtcag tgggaaatca gtgtcatttg gggactgggg
     9601 gtggcaatgg ctatctacct gaccgcaggg gtttccggcg cgcatcttaa tcccgctgtt
     9661 accattgcat tgtggctgtt tgcctgtttc gacaagcgca aagttattcc ttttatcgtt
     9721 tcacaagttg ccggcgcttt ctgtgctgcg gctttagttt acgggcttta ctacaattta
     9781 tttttcgact tcgagcagac tcatcacatt gttcgcggca gcgttgaaag tgttgatctg
     9841 gcaggcactt tctctactta ccctaatcct catatcaatt ttgtgcaggc tttcgcagtt
     9901 gagatggtga ttaccgctat tctgatgggg ctgatcctgg cgttaacgga cgatggcaac
     9961 ggtgtaccac gcggcccttt ggctcccttg ctgattggtc tactgattgc ggtcattggc
    10021 gcatctatgg gcccattgac gggttttgcc atgaacccag cgcgtgactt cggtccgaaa
    10081 gtctttgcct ggctggcggg ctggggcaat gtcgccttta ccggcggcag agacattcct
    10141 tacttcctgg tgccgctttt tggccctatc gttggcgcga ttgtaggtgc atttgcctac
    10201 cgcaaactga ttggtcgcca tttgccttgc gatatctgtg ttgtgaaaga aaaggaaacc
    10261 acaactcctt cagaacaaaa agcttcgctg taatatgact acgggacaat taaacatgac
    10321 tgaaaaaaaa tatatcgttg cgctcgacca gggcaccacc agctcccgcg cggtcgtaat
    10381 ggatcacgat gccaatatca ttagcgtgtc gcagcgcgaa tttgagcaaa tctacccaaa
    10441 accaggttgg gtagaacacg acccaatgga aatctgggcc acccaaagct ccacgctggt
    10501 agaagtgctg gcgaaagccg atatcagttc cgatcaaatt gcagctatcg gtattacgaa
    10561 ccagcgtgaa accactattg tctgggaaaa agaaaccggc aagcctatct ataacgccat
    10621 tgtctggcag tgccgtcgta ccgcagaaat ctgcgagcat ttaaaacgtg acggtttaga
    10681 agattatatc cgcagcaata ccggtctggt gattgacccg tacttttctg gcaccaaagt
    10741 gaagtggatc ctcgaccatg tggaaggctc tcgcgagcgt gcacgtcgta gtgaattgct
    10801 gtttggtacg gttgatacgt ggcttatctg gaaaatgact cagggccgtg tccatgtgac
    10861 cgattacacc aacgcctctc gtaccatgtt gttcaacatc cataccctgg actgggacga
    10921 caaaatgctg gaagtgctgg atattccgcg cgagatgctg ccagaagtgc gtcgttcttc
    10981 cgaagtatac ggtcagacta acattggcgg caaaggcggc acgcgtattc caatctccgg
    11041 gatcgccggt gaccagcagg ccgcgctgtt tggtcagttg tgcgtgaaag aagggatggc
    11101 gaagaacacc tatggcactg gctgctttat gctgatgaac actggcgaga aagcggtgaa
    11161 atcagaaaac ggcctgctga ccaccatcgc ctgcggcccg actggcgaag tgaactatgc
    11221 gttggaaggt gcggtgttta tggcaggcgc atccattcag tggctgcgcg atgagatgaa
    11281 gttgattaac gacgcctacg attccgaata tttcgccacc aaagtgcaaa acaccaatgg
    11341 tgtgtatgtg gttccggcat ttaccgggct gggtgcgccg tactgggacc cgtatgcgcg
    11401 cggggcgatt ttcggtctga ctcgtggggt gaacgctaac cacattatac gcgcgacgct
    11461 ggagtctatt gcttatcaga cgcgtgacgt gctggaagcg atgcaggccg actctggtat
    11521 ccgtctgcac gccctgcgcg tggatggtgg cgcagtagca aacaatttcc tgatgcagtt
    11581 ccagtccgat attctcggca cccgtgttga gcgcccggaa gtgcgcgaag tcaccgcatt
    11641 gggtgcggcc tatctcgcag gcctggcggt tggcttctgg cagaacctcg acgagctgca
    11701 agagaaagcg gtgattgagc gcgagttccg tccaggcatc gaaaccactg agcgtaatta
    11761 ccgttacgca ggctggaaaa aagcggtcaa acgcgcgatg gcgtgggaag aacacgacga
    11821 gtaatgtaaa tgccggatga agcgtttatg ccgcatccgg tagtcctgaa acgtgcgggg
    11881 gcaaccccgc acacatcaat aatcccaccc ttcccctgtg ctacacttcg cgccattcct
    11941 tactgcttag agtttgctat gagacgagaa cttgccatcg aattttcccg cgtcaccgaa
    12001 tctgcggcgc tggctggcta caaatggtta ggacgcggcg ataaaaacac cgcggacggc
    12061 gcggcggtaa acgccatgcg tattatgctc aaccaggtca acattgacgg caccatcgtc
    12121 attggtgaag gtgaaatcga cgaagcaccg atgctctaca ttggtgaaaa agtcggtact
    12181 ggtcgcggcg acgcggtaga tattgctgtt gatccgattg aaggcacgcg catgacggcg
    12241 atgggccagg ctaacgcgct ggcggtgctg gcagtaggcg ataaaggctg cttcctcaat
    12301 gcgccggata tgtatatgga gaagctgatt gtcgggccgg gagccaaagg caccattgat
    12361 ctgaacctgc cgctggcgga taacctgcgc aatgtagcgg cggcgctcgg caaaccgttg
    12421 agcgaactga cggtaacgat tctagctaaa ccacgccacg atgccgttat cgctgaaatg
    12481 cagcaactcg gcgtacgcgt atttgctatt ccggatggcg acgttgcggc ctcaattctc
    12541 acctgtatgc cagacagcga agttgacgtg ctgtacggta ttggtggcgc gccggaaggc
    12601 gtagtttctg cggcggtgat ccgcgcatta gatggcgaca tgaacggtcg tctgctggcg
    12661 cgtcatgacg tcaaaggcga caacgaagag aatcgtcgca ttggcgagca ggagctggca
    12721 cgctgcaaag cgatgggcat cgaagccggt aaagtattgc gcctgggcga tatggcgcgc
    12781 agcgataacg tcatcttctc tgccaccggt attaccaaag gcgatctgct ggaaggcatt
    12841 agccgcaaag gcaatatcgc gactaccgaa acgctgctga tccgcggcaa gtcacgcacc
    12901 attcgccgca ttcagtccat ccactatctg gatcgcaaag acccggaaat gcaggtgcac
    12961 atcctctgat tgatttgatc gattgagcct tccagtcctt cgggactgga atttttttgt
    13021 tcggagaacg aagataaggc aagtcaatca aaacaggaga aaaacatggc tgattgggta
    13081 acaggcaaag tcactaaagt gcagaactgg accgacgccc tgtttagtct caccgttcac
    13141 gcccccgtgc ttccgtttac cgccgggcaa ttcaccaagc ttggccttga aatcgacggc
    13201 gaacgcgtcc agcgcgccta ctcctatgtt aactcgcccg ataatcccga tctggagttt
    13261 tacctggtca ccgtccccga tggcaaatta agcccacgat tggcggcact gaaaccaggc
    13321 gatgaagtgc aggtggttag cgaagcagct ggcttctttg ttctggatga agtaccagat
    13381 tgcgaaacgc tatggatgct ggcaaccggt acagcgattg gcccttattt atcgattctg
    13441 caactaggca aagatttaga tcgcttcaaa aatctggtcc tggttcacgc cgcacgttat
    13501 gccgccgact taagctattt gccactgatg caagaactgg aaaaacgcta cgaaggcaaa
    13561 ctgcgcattc agacggtggt cagtcgggaa acggcagcgg ggtcgctcac cggacggata
    13621 ccggcattaa ttgaaagtgg ggaactggaa agcgcgattg gcctgccgat gaataaagaa
    13681 accagccatg tgatgctgtg cggcaatcca cagatggtgc gcgatacaca acagttgctg
    13741 aaagagaccc ggcagatgac gaaacattta cgtcgccgac cgggccatat gacagcggag
    13801 cattactggt aagcggttac ttatcgataa acggcacgat gagcaaatcc gcactcatct
    13861 tattgatcat cccgcgatat gctggcatca aacggttgat aaatgagtga tgatgaccac
    13921 agacaaggag gtcgcactgc tctttttgca taatttccag cagtgtttcc ggcatttctc
    13981 cgcgttcaat acgcagtttt gtcttcggcc attgaatatt tttcgtcagt ttatacagct
    14041 tgttatccga cttattcttc aacaattgaa gaatatcttc tgttgcaggg aagtagatac
    14101 ccggatacaa ctcgcttaag ccatcatcaa tatgaattaa cgtcaggtga gcgtcattat
    14161 gtctggcgag ctccagagct ttattcacca gtaaggcatc ttcttcattc ccggaaattg
    14221 ccacgccaat gtgtttataa gccatgttta actccttcta aagcaactcc atcaagctaa
    14281 cgaacgcagt gataactcaa aaataatcat ctcggcctgg catgagaaag tgaaggccgc
    14341 atcaagctca acttgcccct ccagatcctt aatcttgtag gccacgctgg ccccttcatc
    14401 tacagaggat gcagcggcgg caagctcttt cagtttggtc ataaaagact ctgcttcttc
    14461 gcgggtggca aaaaagcgcg aaaacttact ggtacagttg tcgttatcaa tcaccgtacc
    14521 gatatcaatc gcacatcctt tagtactgca tttatctacg acatctttca tagggcacct
    14581 ctgtatattc gcctctctgt cgattacgac ttaagaataa tgcgccgtta agggaagaac
    14641 aaacaagtaa tgatcttatg gattaccatt ttttttgata ttgcttaagg tgaagagttc
    14701 agaaggattt tccgggtatt gtcatttgca tcgtggaaat aacaatcccg gaaaaaaata
    14761 gcagtaagag atatattaaa ggttaattac ttgatttatt gtcggcttta tacttcacat
    14821 cctgagtatc tttaccatat ttattttcgc cttgtgtgcc aacaaacgca ccaagatcga
    14881 taagcatcat caccagaatc aacgtcggga caaaacaccc caccgcccat tgccagacac
    14941 ccggtaaaat cgcccagtta cccgccagca gcatccacgc cacaatcatc agaaatgccc
    15001 atgcgccgga acgcccgcga tcatgcaagc gcttaacagt tactgccgct gttggccaaa
    15061 gcaagcacac aaggcaaaac gccgcggtct gaatatcgag taaattttta cccgccagtg
    15121 agaaaagcac cagcatgcct gcgaaccaca ggcctatcca aatccagaaa tcacggcgtc
    15181 caatacgccc tttaaatgag aataaccatt gctgtatggt catgtaagtt ccttgatggt
    15241 tgtcttttcc aggattctac ccttttgaca agggcgacag atatcgtttt aatcggagcc
    15301 agtcataaca aaaggtactg tcaatgaagc cagggtgtac gctgtttttt ctcttatgtt
    15361 ctgcattaac cgttacaaca acggcgcatg cacaaacacc agatacggca acgaccgcgc
    15421 cttatctgct ggctggagcc cctactttcg atctctccat cagccagttt cgagaagact
    15481 ttaacagcca gaatcctagc ctgccactga acgaatttcg tgccatcgac agcagtcccg
    15541 acaaagccaa tctcactcgt gctgcaagta aaattaacga gaacttgtat gcttctacag
    15601 cgctggagcg cggtacctta aaaatcaaaa gcattcaaat gacctggcta cccatccagg
    15661 ggccagagca aaaagcagcg aaagcgaaag ctcaggaata catggcagca gtgatccgca
    15721 cactcacccc attaatgacc aaaacacaaa gccagaaaaa actgcagtcg ctgctaacgg
    15781 cggggaaaaa caaacgttat tacaccgaga cagaaggtgc actgcgttat gttgtcgcgg
    15841 acaacggcga aaaggggctg accttcgctg ttgaaccgat taagctggcg ctatctgaat
    15901 cgcttgaagg tttgaataaa tgacaaaaag caaagccttt gtgccgatga atctctatac
    15961 tgtttcacag acctgctgcc ctgcggggcg gccatcttcc tttattcgct tataagcgtg
    16021 gagaattaaa atgcgacatc ctttagtgat gggtaactgg aaactgaacg gcagccgcca
    16081 catggttcac gagctggttt ctaacctgcg taaagagctg gcaggtgttg ctggctgtgc
    16141 ggttgcaatc gcaccaccgg aaatgtacat cgatatggcg aagcgcgaag ctgaaggcag
    16201 ccacatcatg ctgggtgcgc aaaacgtgga cctgaacctg tccggcgcat tcaccggtga
    16261 aacctctgct gctatgctga aagacatcgg cgcacagtac atcatcatcg gccactctga
    16321 acgtcgtact taccacaaag agtctgacga actgatcgcg aaaaaattcg cggtgctgaa
    16381 agagcagggt ctgactccgg ttctgtgcat cggtgaaacc gaagctgaaa acgaagcggg
    16441 caaaactgaa gaagtttgcg cacgtcagat cgacgcggta ctgaaaactc agggtgctgc
    16501 ggcattcgaa ggtgcggtta tcgcttacga acctgtatgg gcaatcggta ctggcaaatc
    16561 tgcaactccg gctcaggcac aggctgttca caaattcatc cgtgaccaca tcgctaaagt
    16621 tgacgctaac atcgctgaac aagtgatcat tcagtacggc ggctctgtaa acgcgtctaa
    16681 cgctgcagaa ctgtttgctc agccagacat cgacggcgcg ctggttggcg gtgcgtctct
    16741 gaaagctgac gctttcgcag taatcgttaa agctgcagaa gcggctaaac aggcttaagt
    16801 ctgacaggtg ccggatttca tatccggcac ttactttcct taactcttcg ccttaacgca
    16861 aaatctcaca ctgatgatcc tgaatttcct cggctgaagc acggttaagc gtcagtagat
    16921 ttcgttgtgt cgccagcaat acaaatgagt tatcactctg ccgtaccatc gccagcccgt
    16981 agcttcccat atgttcccgc gcctcaggta cttcttctgc cagcatcata aatgggctgc
    17041 gttgtaccag ttcgctttcc gttacccgac gcgccaggta ttcatgcccg cgcaaaccac
    17101 ctggcagtgg caaccagcgg ctgctgatgt tcgccagatt gttatccagt tgttcgcgca
    17161 catcaggacg aatacaagag atatgaatat gaaaatggtt ttgcgtacgc ccggtgcggg
    17221 agttgatcgc caaagaaacc gcgcgatcgg gaaccggctg gccgtacttt ttgctcataa
    17281 aatcacgcgc ctgccaggcc aaccaaaaga agttcggcgt tgaaggatcg gtcaacaaag
    17341 gactttcagt accgttaata cgatacgttg gcatcaacag atattgcagt gggccattaa
    17401 gatcttttaa aaccacgtat ccggcattgg gtttgacttc cgcacatggc gaaggatttt
    17461 gattttgctg ctgattgggc aaacattcct caaggacaat cttacgtaat gtatccgact
    17521 cttcaccggt taatttccag taaccaatac cggcagccac aacggcgata actatcatca
    17581 ccaaaaaaag aagacccgct tttttcatct ttttttccct gtacctcaaa gagatgcaag
    17641 ggtaacgcaa aatcgtgaca aataaaaaac ccggtcgcga gaaaccaccg ggtttgataa
    17701 ttatcctggg gaaatcagcg tttgctgatc tgatcgaacg taccgccgtt agcaaaatgc
    17761 tctttttgcg ctttcgtcca gccgccgaac tcttcatcaa tggtgaataa cttcagcttt
    17821 ggaaacgcat tttcgtactt tttcgctact tcagcgtcgc gcggacggta gtagtttttc
    17881 gcggcaattt cctgaccttc tggcgagtag agatatttca ggtaggcttc cgccacctct
    17941 ttggtgcctt ttttctcgac cactttatcg accaccgaca cggttggctc tgcgaggata
    18001 gactcactcg gcgtgacgat ttcgaattta tctttcccca gttcattcgc tgccagcaga
    18061 gcttcgtttt cccaggcgat cagtacatcg ccaatcccgc gctcgacaaa ggtgttagtg
    18121 gagccgcgcg cgccagaatc cagaacttcg acgtttttat acagtgcccg aacaaaatcc
    18181 tgtgcttttg cctgatcgtt gttgttgtga tgcagcgcgt agccccaggc agccaggtag
    18241 ttccagcgtg cgccaccaga gcttttcgga ttaggcgtga tcaccgaaac acccggttta
    18301 atcagatcgt tccagtcatg gatctgcttc ggattgccct tacgcaccag gaaaacaatg
    18361 gtggaagtgt acggtgcgga gttatccggc agacgtttga tccactcttt atcaatccgc
    18421 ccgcgttccg caattgcgtc cacgtcatag gccagagcca gcgtgacaac atcagcttca
    18481 ataccgttga ttaccgacgt cgcttgttta cctgaaccac cgtgtgactg acgcatcacc
    18541 acgttatcac cagtttgctg tttccagtgg gcgctgaatg ccttgttgta ctgttcgtac
    18601 aattcgcgcg ttggatcata tgaaacgtta agaagctgaa tatcctttgc cataacgctg
    18661 gttgccgcca gcaaaaatgt taaccctacg ccccacttgt tcatcgcccg actctcttat
    18721 gttgtgttgt gatgagcaaa gcgtgccaga aggttaacca aacattaaag aataaaaaaa
    18781 gattggctat aacttgcggg tatatgttga gggattaaaa aggcggaaga aatccgcctc
    18841 atattgctga caaagtgcgc tttgtccatg ccggatgcgg cgcgaacgcc ttatccggcc
    18901 tacaaaagtt tgcaaattca ataaattgca gaattcatgt aggcctgata agcgaagcgc
    18961 atcaggcatt tttgcttctg tcatcggttt caggctaaag gaatctgcct ttttccgaaa
    19021 tcattaatac agttttttcg cgcagtccag ccagtcacct ttgaacggac gcttcatgtt
    19081 ttcgatagcg tcgatgatgt cgtggtgaac cagctgttcg ttctggatac ctacacaacg
    19141 accgccgtaa cctgccagca gcagatcgat agcgtaagcg cccatacggg aagccagaat
    19201 acggtcgtaa ggcaccggag aaccaccgcg ctggatgtgg cccagcacag ttgcgcgggt
    19261 ttcacgaccg gtttctttct cgatgaaatg cgccagttcg tcaacatcac acatatgttc
    19321 ggtaatcgcc acgatcgcgt gttttttacc tttcgcgata cccgctttga tttcgtttac
    19381 caggtcttca cggctgaatt caacttccgg aaccacaacg aattcacagc caccggcaat
    19441 ggccgcagcc aacgtcagat cgccacaata acggcccatc acttccacca cggaaatacg
    19501 ctggtgagaa gaagaggtgt cacgcagacg gtcgatcgct tctacaacgg tgctcagcgc
    19561 agtgaagaaa ccgatagtgt agtcagtgcc tttgatgtcg ttgtcgatag tgcccggcag
    19621 accgatgcac gggaagccca tttcggtcag acgcattgca cccatgtagg aaccgtcacc
    19681 gccgataacc accagcgcgt cgataccacg ttttttcagg ttttcgatag ccacggcgcg
    19741 gatgttctcg tcgcggaatt ccgggaaacg cgcagaaccg aggaacgtac cgccacggtt
    19801 gatcatgtca gacacgctgt aacggtctag ctgtaccata cggtcttcat acagacccag
    19861 atagccgtca taaataccca ttacttccag accttctgtc agcgcagaac gaacaacccc
    19921 gcgaattgcg gcgttcatgc ctggcgcatc accgccgctt gtcaacacac cgattttctt
    19981 aatcatgact acctctgaac tttggaatgc aaaatgaaat ctgttgccgg aagtcttctt
    20041 gcacatcgaa gtgatccaac gaatgtgcaa atagtataac aatcacttcc tgctgaattg
    20101 attcaggtca ggccaaatgg cggtatttta tacacaaaat gcgggtctgg ctctctttta
    20161 tactgattat gaaagcatag accgtttacc ctccctgggt acgacggaac aggggtcctg
    20221 atggataatt acatccgatc ccggaaaacg ccgtaaaata gcctgctcta cctgatccgc
    20281 caccatatgt gcctgaacca aaggcagaga gtcttccatt tccaaatgaa tctgaataaa
    20341 gcgggtcggc cctgactgcc gcgtgcgaag atcgtgagcg ccgctaacac ccggccagga
    20401 agtcacgata tcaataattt cttgccgttc ctcatcaggc aatgcgcgat ccagtaatga
    20461 ctgtaccgcc tcatatccca tgcgtaacgc gctatataaa atatagatgc cgattcccaa
    20521 tgcaaacaga gcatcggcgc gatgccagcc gtaccaggac aaccccagcg ccagcagaat
    20581 tgcgccgttc atcataacat cagactggta atgtagcata tcagcccgca ccgcctggct
    20641 ttgcgtccgg cgcaccaccc aacgctgaaa cgagacaagg ataatcgtac aaattagcgc
    20701 cacaattgtc acgataaccc cgacgcctgg atctatcatc ggtgttggag atatcagatg
    20761 ttgaataccc gtcaaaaaca ggaatagtgc cgaaccggag ataaacatac tttgcgccag
    20821 cgccgcgagg gactctgctt taccgtgacc aaacgagtga ttatcgtcgg caggttgcag
    20881 ggaatatcgc accaccagta aattcgtcaa cgacgcgccg atatccacca gcgaatccac
    20941 cagcgcggcg agaatactca ccgacccggt ataccaccat gcaaaaattt taatcagcaa
    21001 tagcaacgaa gccatcgccg tcgcagcaat cgccgcccga ctgaccagcc gtccataaga
    21061 ttgattcata aatactcccg ctatcaactg acgctagtat aacggaagca aatcatctgc
    21121 aatgcattaa gcagcaggca aattgaggat aaaaaaaacc ccacatcatg tgggggaaga
    21181 cagggatggt gtctatggca aggaaaacag ggtttactac tgggaacgtg agttgctact
    21241 actcaatagc ttcaacgatg aacttttttg ccattgcgtc acgtcacgca actgctccat
    21301 tcgttgttga tgtttctcgt ttaaaaccgc ttgctgctcc ggcgttaaca ggcgatacat
    21361 ttggttgcgg acttttgcca tctcaacctg acgagcaatt tgctcattcg ccattttttc
    21421 tgcctgtgcg cgcacagcgt tttcatcaaa attttctgcg gtgacaaggc gatgcattgt
    21481 ctccagttcg ctaacattaa caggaggctg ttcgtgccgg gcctgttgca taagatctcg
    21541 catctgctga cgctgatgtt cggttaaact tatgccgtcg aacatatggc tctgcgtact
    21601 gcgctgcgta ctgcgctgcg taagttcttc acccggatgc cagttatcgc ctgaaccgac
    21661 ttcagcagcg tggcttaatg aactgactgc cagcgttgaa gccatgacgg cagcggtaac
    21721 tatgcgcatc atttgctccc aaaatctttc tgtcgcgatt caacgataga gagtttacga
    21781 ttcaggctgc aaacatgcgt cagggggtgt aaaacaacgt aaagtcatgg attagcgacg
    21841 tctgatgacg taatttctgc ctcggaggta tttaaacaat gaataaaatc ctgttagttg
    21901 atgatgaccg agagctgact tccctattaa aggagctgct cgagatggaa ggcttcaacg
    21961 tgattgttgc ccacgatggg gaacaggcgc ttgatcttct ggacgacagc attgatttac
    22021 ttttgcttga cgtaatgatg ccgaagaaaa atggtatcga cacattaaaa gcacttcgcc
    22081 agacacacca gacgcctgtc attatgttga cggcgcgcgg cagtgaactt gatcgcgttc
    22141 tcggccttga gctgggcgca gatgactatc tcccgaaacc gtttaatgat cgtgagctgg
    22201 tggcacgtat tcgcgcgatc ctgcgccgtt cgcactggag cgagcaacag caaaacaacg
    22261 acaacggttc accgacactg gaagttgatg ccttagtgct gaatccaggc cgtcaggaag
    22321 ccagcttcga cgggcaaacg ctggagttaa ccggtactga gtttaccctg ctctatttgc
    22381 tggcacagca tctgggtcag gtggtttccc gtgaacattt aagccaggaa gtgttgggca
    22441 aacgcctgac gcctttcgac cgcgctattg atatgcacat ttccaacctg cgtcgtaaac
    22501 tgccggatcg taaagatggt cacccgtggt ttaaaacctt gcgtggtcgc ggctatctga
    22561 tggtttctgc ttcatgatag gcagcttaac cgcgcgcatc ttcgccatct tctggctgac
    22621 gctggcgctg gtgttgatgt tggttttgat gttacccaag ctcgattcac gccagatgac
    22681 cgagcttctg gatagcgaac agcgtcaggg gctgatgatt gagcagcatg tcgaagcgga
    22741 gctggcgaac gatccgccca acgatttaat gtggtggcgg cgtctgttcc gggcgattga
    22801 taagtgggca ccgccaggac agcgtttgtt attggtgacc accgaaggcc gcgtgatcgg
    22861 cgctgaacgc agcgaaatgc agatcattcg taactttatt ggtcaggccg ataacgccga
    22921 tcatccgcag aagaaaaagt atggccgcgt ggaactggtc ggtccgttct ccgtgcgtga
    22981 tggcgaagat aattaccaac tttatctgat tcgtccggcc agcagttctc aatccgattt
    23041 cattaactta ctgtttgacc gcccgctatt actgctgatt gtcaccatgt tggtcagtac
    23101 gccgctgctg ttgtggttgg cctggagtct ggcaaaaccg gcgcgtaagc tgaaaaacgc
    23161 tgccgatgaa gttgcccagg gaaacttacg ccagcacccg gaactggaag cggggccaca
    23221 ggaattcctt gccgcaggtg ccagttttaa ccagatggtc accgcgctgg agcgcatgat
    23281 gacctctcag cagcgtctgc tttctgatat ctctcacgag ctgcgcaccc cgctgacgcg
    23341 tctgcaactg ggtacggcat tactgcgccg tcgtagcggt gaaagcaagg aactggagcg
    23401 tattgaaacc gaagcgcaac gtctggacag catgatcaac gatctgttgg tgatgtcacg
    23461 taatcagcaa aaaaacgcgc tggttagcga aaccatcaaa gccaaccagt tgtggagtga
    23521 agtgctggat aacgcggcgt tcgaagccga gcaaatgggc aagtcgttga cagttaactt
    23581 cccgcctggg ccgtggccgc tgtacggcaa tccgaacgcc ctggaaagtg cgctggaaaa
    23641 cattgttcgt aatgctctgc gttattccca tacgaagatt gaagtgggct ttgcggtaga
    23701 taaagacggt atcaccatta cggtggacga cgatggtcct ggcgttagcc cggaagatcg
    23761 cgaacagatt ttccgtccgt tctatcggac cgatgaagca cgcgatcgtg aatctggcgg
    23821 tacaggattg gggctggcga ttgttgaaac cgccattcag cagcatcgtg gctgggtgaa
    23881 ggcagaagac agcccgctgg gcggtttacg gctggtgatt tggttgccgc tgtataagcg
    23941 gagttaaact ccgcatttgt aggcaggata aggcgtttac gccgcatccg gcatttgagc
    24001 aggatgcctg atgcgacgct gatagcgtct tatcaggcct acactccggg ggttattttc
    24061 cccacaaccg gcgggaaaaa tcttcgatct tgccgctcag tcggcgcttt tgcatcgtcc
    24121 gcgtccagct tttcgataac ccggcggcgg agagtaaacg gtgatactga tcgtcatcaa
    24181 acggcatatg ccatgcgatg gcggcagcct cctgcacggt cacatcgctg acacgggaaa
    24241 ccaactccag cggcgcgtcc gcagacactt tccctggcgc tatcacgcta tacaaccacc
    24301 ccaccttacc tgtgttttgc atcaactgcg ctatatcgct gatatcaaaa tggtaattga
    24361 gtttatagca aggcgagcgc ggctggctga cctgaattaa tgcctctccc cagcggaaaa
    24421 tatcgcccat ataaacatta ctttccgtca ggccgtcggt tgagaggttt tcaccaaacg
    24481 caggcgcaac aaacaactcc gcctgttccg gaaattcccg cgcccagtag agataatgct
    24541 cacgaggata atgacacagc gctctgtctg gcccaccatg aacctttttc tccgcctgct
    24601 cgtcaccttc cagccccagc tccgtcagca tcaactcacc atcaacctgg attttggcaa
    24661 ttgcgctggg tttgccttcg ggataagcct gaatcttgcc tgtgtataca tcaaccggat
    24721 atcgcatctg ttccctctcc acgagaaatt tccccattag accatattta ttttatgtca
    24781 ggcgtgctgt tttcgcggta agtatccagg caggaactca atagcaaacc ggacgaacgt
    24841 tagttcatcc ggctgtgggg attaaggaag caggtttatt tcaccgtacc taaaatttcg
    24901 attttcgctg ctctggcttt gactttacgt gaccagatag aggtaagaat tggcaccaga
    24961 atcgaagtca caatgaccgc cgtcgctacc agcgaagttg ctgccggagc catcggttta
    25021 aacgcaggca ccatttctgc aatcagcata ggtgtcgcta ccgcggcccc tgcggaactg
    25081 gaagcggcaa ttccggcagt gccatcgcca ccgccaatca atttatcggc gataatcaac
    25141 ggaataccgg tcacgataat tactgccaca cccaacagga tccccagcaa accagtctgg
    25201 gcaatcacag tcaaatcaat ggtattgccc agcgcgaagg caaagaatgg aatcagcgtt
    25261 tgcaccgctt tgctgaaaaa ttctcgtaat tcagggtcaa ggttccccag ggcaaagccc
    25321 accaggaacg gtaatactgc gccgacgaaa acatgcggtt caaacgaggc aatccctgca
    25381 gtgcccagaa taatcatcgt catgagcggc ccggactcca acgacatcaa cacaaatgcc
    25441 ccagcttctt cttttgtgcc gtactgctgc atgatggaag cgtaaagtcc gccattggtc
    25501 atatccatcg ccgccaccag cgccagcgtt gaaagtccgg caaagaatcc aacttcaaca
    25561 ccatgttccg gaataatgcg cgaggcaatt gccgcaacca cccacgcgac ggcaattttt
    25621 gttaccacca gcgtaccgga tttacgcaat accgttcccg tcgcgcttaa ttttattgac
    25681 gcccccatgc aaaaaaacca caccgccaga atggacaccg taccggtaat catcccgttg
    25741 gtgaatgatc caaaatattt ccccgccccc ggcgagaagg tgtggcacag tgcgccaagg
    25801 aatagcggga cgagcatcat ccccccccgg gattttctca atcgagcgtt ttatctgcat
    25861 ttccatcacc tgtcggcata tttttaaggg ctgatgcgta attcctcaat ggagcatcag
    25921 tgttgatgga aacgaagata gacattcatt taatcaataa aagtgatcaa aaccacatca
    25981 attgaaacgc tgttttattt ttatcggatc attgttttta ttttgaaatt gatcacaaaa
    26041 aaaccaccgc cgttggcgat ggttcatttt ttaagctgat atgcggcgta aacgcctcat
    26101 tgcagcaggc ggcaaatgat tattttttcg ccgcgaaacg tgccgctgct tcatcccagt
    26161 tcaccacgtt ccagaactct ttgatgtagt ccggacggcg gttctggaat ttcaggtagt
    26221 aagcatgttc ccacacatcc aggcccagaa tcgggaagcc ggaagcgcca gaaatagctt
    26281 cacccatcag cggggaatcc tggttagcag tagaaaccac cgccagttta tcgcctttca
    26341 gcaccagcca tgcccagccg gaaccaaagc gggaagctgc cgctttctca aattctgctt
    26401 tgaagttatc aacggagccg aagtcacgtt cgatagccgc tttcaggtca ccctgtaagg
    26461 tggtgccttt tttcagacct ttccagaaca ggctgtggtt agcgtgaccg ccagcgttgt
    26521 tgcgcagtac ggttttcttg tctgctggca gctggtccag tttggtgatc agctcttcaa
    26581 ccggcaggtt ggcaaattct ggcaggcttt ccagcgccgc gttggcgttg ttgacgtagg
    26641 tctgatggtg tttggtgtgg tggatttcca tggtctgctt atcgaagtgc ggttccaggg
    26701 catcgtaagc atacggcagg gatggcaggg tatagctcat attcatctcc agtattgtcg
    26761 ggcggccgat tgttaatgcg cgtaagcagt tggttcatta tagttaatta aatgatattg
    26821 aaaatgatta tcaatgccgt acttttcgta agggtatggt tttgcaggaa aattcccgag
    26881 atgtgaagca aatcacccac ttaatgccgt gattgccagt aaatcgacaa cggcggcaac
    26941 aggcgaaagg ttaatcgaca gcacgatttt tacactcatc tcgtcggaga tgtgacgcga
    27001 cgaaaaatga tgaggataag aagatgagta acgcgattac gatggggata ttttggcatt
    27061 tgatcggcgc ggccagtgca gcctgttttt acgctccgtt caaaaaagta aaaaaatggt
    27121 catgggaaac catgtggtca gttggtggga ttgtttcgtg gattattctg ccgtgggcca
    27181 tcagcgccct gttactgccg aatttctggg cgtattacag ctcgtttagt ctctctacgc
    27241 tgctgcctgt ttttctgttc ggcgctatgt gggggatcgg taatatcaac tacggcctga
    27301 ccatgcgtta tctcggcatg tcgatgggaa ttggcatcgc cattggcatt acgttgattg
    27361 tcggtacgct gatgacgcca attatcaacg gcaatttcga tgttctgctt aataccgaag
    27421 gcggacgcat gacgttgctc ggcgttctgg tggcgctgat tggtgtaggg attgtgactc
    27481 gcgccgggca gttgaaagag cgcaaaatgg gcattaaagc cgaagagttc aacctgaaaa
    27541 aagggctggt gctggcggtg atgtgcggca ttttctctgc cggtatgtct tttgcgatga
    27601 acgccgcaaa accgatgcat gaagccgctg ccgcactggg cgtcgatcca ctgtatgtcg
    27661 ctctgccaag ctatgtcatc atcatgggcg gcggcgcgat catcaacctc ggtttctgct
    27721 tcattcgtct ggcaaaagtg aaggatttgt cgctaaaagc cgacttctcg ctggcaaaac
    27781 cgctgatcat tcacaatgtg ttactctcag cactgggcgg gttgatgtgg tatctgcaat
    27841 tctttttcta tgcctggggc cacgcccgca ttccggcgca gtatgactac atcagttgga
    27901 tgctgcatat gagtttctat gtattgtgcg gcggtatcgt cgggctggtg ctgaaagagt
    27961 ggaacaatgc aggtcgccgt ccggtaacgg tgttgagcct cggttgtgtg gtgattattg
    28021 tcgccgccaa catcgtcggc atcggcatgg cgaattaatc tttctgcgaa ttgagatgac
    28081 gccactggct gggcgtcatc ccggtttccc gggtaaacac caccgaaaaa tagttactat
    28141 cttcaaagcc acattcggtc gaaatatcac tgattaacag gcggctatgc tggagaagat
    28201 attgcgcatg acacactctg acctgccgca gatattgatt gatggtcatt ccagtctgct
    28261 ggcgaaattg ctgacgcaaa acgcgctcac tgcacgatgc ctcatcacaa aatttatcca
    28321 gcgcaaaggg acttttcagg ctagccgcca gccgggtaat cagcttatcc agcaacgttt
    28381 cgctggatgt tggcggcaac gaatcactgg tgtaacgatg gcgattcagc aacatcacca
    28441 actgcccgaa cagcaactca gccatttcgt tagcaaacga cacatgctga ctactttcat
    28501 gctcaagctg accgataacc tgccgcgcct gcgccatccc cacgctacct aagcgccagt
    28561 gtggttgccc tgcgctggcg ctaaatcccg gaatcgctcc ctgccagtca agattcagct
    28621 tcagccgctc cgggcaataa ataatattct gcaaaaccag atcgttaacg gaagcgtagg
    28681 agtgtttatc gtcagcatga atgtaaaaga gatcgccacg ggtaatgcga taagggcgat
    28741 cgttgagtac atgcagacca ttaccgcgcc agacaatcac cagctcacaa aaatcatgtg
    28801 tatgttcggc aaaaacatct tgcggataac ggtcagccac agcgactgcc tgctggtcgc
    28861 tggcaaaaaa atcatctttg agaagtttta actgatgcgc caccgtggct acctcggcca
    28921 gagaacgaag ttgattattc gcaatatggc gtacaaatac gttgagaaga ttcgcgttat
    28981 tgcagaaagc catcccgtcc ctggcgaata tcacgcggtg accagttaaa ctctcggcga
    29041 aaaagcgtcg aaaagtggtt actgtcgctg aatccgcagc gataggcgat gtcagtaacg
    29101 ctggcctcgc tgtgacgtag cagatgtcgg gccttcatca gtcgcaggcg gttcaggtaa
    29161 cgctgaggcg tcagtcccgt ttgctgctta agctgccgat gtagcgtacg cagtgaaaga
    29221 gaaaattgat ccgccacggc atcccaattc acctcatcgg caaaatggtc ctccagccag
    29281 gccagaagca agttgagacg tgatgcgctg ttttccaggt tctcctgcaa actgctttta
    29341 cgcagcaaga gcagtaattg cataaacaag atctcgcgac tggcggtcga gggtaaatca
    29401 ttttcccctt cctgctgttc catctgtgca accagctgtc gcacctgctg caatacgctg
    29461 tggttaacgc gccagtgaga cggatactgc ccatccagtt cttgtggcag caactgattc
    29521 agcccggcga gaaactgaaa tcgatccggc gagcgataca gcacattggt cagacacaga
    29581 ttatcggtat gttcatacag atgccgatca tgatcgcgta cgaaacagac cgtgccaccg
    29641 gtgatggtat agggctgccc attaaacaca tgaatacccg tgccatgttc gacaatcaca
    29701 atttcatgaa aatcatgatg atgttcagga aaatccgcct gcgggagccg gggttctatc
    29761 gccacggacg cgttaccaga cggaaaaaaa tccacactat gtaatacggt catactggcc
    29821 tcctgatgtc gtcaacacgg cgaaatagta atcacgaggt caggttctta ccttaaattt
    29881 tcgacggaaa accacgtaaa aaacgtcgat ttttcaagat acagcgtgaa ttttcaggaa
    29941 atgcggtgag catcacatca ccacaatgaa gcaaattgtg aacatcatca cgttcatctt
    30001 tccctggttg ccaatggccc attttcctgt cagtaacgag aaggtcacga attcaggcgc
    30061 tttttagact ggtcgtaatg aaattcagca ggatcacatt atgacctttc gcaattgtgt
    30121 cgccgtcgat ctcggcgcat ccagtgggcg cgtgatgctg gcgcgttacg agcgtgaatg
    30181 ccgcagcctg acgctgcgcg aaatccatcg ttttaacaat ggcctgcata gccagaacgg
    30241 ttatgtcacc tggaatgtgg atagcctgga aagtgccatt cgccttggat taaacaaggt
    30301 gtgcgaggaa gggattcgta tcgatagcat tgggcttgat acctggggcg tggactttgt
    30361 gctgctcgac caacagggtc agcgtgtggg cctgcccgtt gcttatcgcg atagccgctc
    30421 caatggccta atggcgcagg cacagcaaca actcggcaaa cgcgatattt atcaacgtag
    30481 cggcatccag tttctgccct tcaatacgct ttatcaactg cgtgcgctga cggagcaaca
    30541 acctgaactt attccacaca ttgctcacgc tctgctgatg ccggattact tcagctatcg
    30601 cctgaccggc aagatgaact gggaatatac caatgccacc accacacaac tggtcaatat
    30661 caatagcgac gactgggacg agtcgctact ggcgtggagc ggggccaaca aagcctggtt
    30721 tggtcgcccg acgcatccgg gtaatgtcat tgggcactgg atttgcccgc agggtaatga
    30781 gattccggtg gtcgccgttg ccagccatga taccgccagc gcggttatcg cctcgccgtt
    30841 aaacggttca cgcgctgctt atctctcttc tggcacctgg tcattgatgg gcttcgaaag
    30901 ccagacgcca tttaccaatg acacggcgct ggcagccaac ataaccaatg aaggcggggc
    30961 ggaaggtcgc tatcgggtgc tgaaaaatat tatgggctta tggctgcttc agcgagtgct
    31021 tcaggagcgg caaatcaacg atctcccggc gcttatcgcc gcgacacagg cacttccggc
    31081 ctgccgcttc atcatcaatc ccaatgacga tcgctttatt aatcctgagg cgatgtgcag
    31141 cgaaattcag gctgcgtgtc gggaaacggc gcaaccgatc ccggaaagtg atgctgaact
    31201 ggcgcagcta cgcggtgaag atttctcgca actgcatatt gtcggcggcg gctgccagaa
    31261 cacgctgctc aaccagctat gtgccgatgc ctgcggtatt cgggtgatcg ccgggcctgt
    31321 tgaagcctcg acgctcggca atatcggcat ccagttaatg acgctggatg aactcaacaa
    31381 tgtggatgat ttccgtcagg tcgtcagcac caccgcgaat ctgaccacct ttacccctaa
    31441 tcctgacagt gaaattgccc actatgtggc gcagcttcac tctacacgac agacaaagga
    31501 gctttgcgca tgaccactca actggaacag gcctgggagc tggcgaaaca gcgtttcgcg
    31561 gcggtgggga ttgatgtcga ggaggcgctg cgccaacttg atcgtttacc cgtttcaatg
    31621 cactgctggc agggcgatga tgtttccggt tttgaaaacc cggaaggttc gctgaccggg
    31681 gggattcagg ccacaggcaa ttatccgggc aaagcgcgta atgccagtga gctacgtgcc
    31741 gatctggaac aggctatgcg gctgattccg gggccgaaac ggcttaattt acatgccatc
    31801 tatctggaat cagatacgcc agtctcgcgc gaccagatca aaccagagca cttcaaaaac
    31861 tggggtgaat gggcgaaagc caatcagctc ggtctggatt ttaacccctc ctgcttttcg
    31921 catccgctaa gcgccgatgg ctttacgctc tcgcatcccg acgacagcat tcgccagttc
    31981 tggattgatc actgcaaagc cagccgtcgc gtttcggcct attttggcga gcaactcggc
    32041 acaccatcgg tgatgaacat ctggatcccg gatggtatga aagatatcac cgttgaccgt
    32101 ctcgccccgc gtcagcgtct gctggcagca ctggatgagg tgatcagcaa gaagctaaac
    32161 cctgcgcacc atatcgacgc cgttgagagc aaattgtttg gcattggcgc agagagctac
    32221 acggttggct ccaatgagtt ttacatgggg tatgccacca gccgccagac tgcgctgtgc
    32281 ctagacgccg ggcacttcca cccgactgaa gtgatttccg acaagatttc cgccgccatg
    32341 ctgtatgtgc cgcagttgct gctgcacgtc agccgtccgg ttcgctggga ctgcgatcac
    32401 gtagtgctgc tggatgatga aacccaggca attgccagtg agattgtgcg tcacgatctg
    32461 tttgaccagg tgcatatcgg ccttgacttc ttcgatgcct ctatcaaccg cattgccgcg
    32521 tgggtcattg gtacacgcaa tatgaaaaaa gccctgctgc gtgcgttgct ggaacctacc
    32581 gctgagctgc gcaagctgga agcggcgggc gattacactg cgcgtctggc actgctggaa
    32641 gagcagaaat cgttgccgtg gcaggcggtc tgggaaatgt attgccaacg tcacgatacg
    32701 ccagcaggta gcgaatggct ggagagcgtg cgggcttatg aaaaagaaat tttgagccag
    32761 cgtgggtaaa cactgccgga tgcggcgcga gcgccttatc cggcctacgg gtcggcaaca
    32821 gttgtaggcc tgataagacg cgacagcgtc gcatcaggca ttgattgccg gatgcggcac
    32881 gagtgcctta tccggcctac aggtcggcaa tagttgtagg cctgataaga cgcgacagcg
    32941 tcgcatcagg cattgattgc cggatgcggc acgagcgcct tatccggcct acgggtcgtg
    33001 catctgacaa caccgaattt acaggaacac agaacatgca aaacattacc cagtcctggt
    33061 ttgtccaggg aatgatcaaa gccaccaccg atgcctggct gaaaggctgg gacgaacgca
    33121 acggcggcaa cctgacgctg cgcctggatg acgccgatat cgcgccttat aaagacaacg
    33181 tccatgcaca accgcgctat atcccgctca gccagcccat gcctttactg gcaaatacac
    33241 cgtttattgt caccggctcc ggcaaattct tccgtaacgt ccagctcgat cctgcagcta
    33301 acttaggcgt cgtaaaagtc gacagcgatg gcgcgggcta ccatattctc tgggggttaa
    33361 ccaacgaagc cgtcccgaca tccgaactcc cggcgcactt tctctcccac tgcgagcgca
    33421 ttaaagccac caacggtaaa gatcgggtga tcatgcactg ccacgccacc aacctgatcg
    33481 ccctcaccta tgtactggaa aacgacaccg cggtctttac tcgccaactg tgggaaggca
    33541 gcaccgagtg tctggtggta ttcccggatg gcgttggcat tctgccgtgg atggtgcccg
    33601 gcacggacga aatcggccag gcgaccgcac aagagatgca aaaacattcg ctggtgttgt
    33661 ggcccttcca cggcgtcttc ggcagcgggt cgacgttgga tgaaacattc ggtttaatcg
    33721 acaccgcaga aaaatcagca caagtattag tgaaggttta ttcgatgggc ggcatgaaac
    33781 agaccatcag ccgtgaagag ttgatagcgc tcggcaagcg ctttggcgtt acaccactag
    33841 ccagtgcgct ggcgctgtaa ggagcaaaca tgatccgcaa agcctttgtc atgcaggtta
    33901 accccgacgc ccacgaagag tatcagcgtc ggcataaccc catctggccg gaactggaag
    33961 cagtgttgaa atctcacggt gcgcataact acgccattta cctcgacaaa gcgcgtaatc
    34021 tgctgtttgc cacggtagag attgaatctg aagaacggtg gaatgcggta gccagtgctg
    34081 atgtttgcca acgttggtgg aaatatatga ccgatgttat gcccgctaac ccggataaca
    34141 gcccggtgag tagcgagctg
//
LOCUS       DAJTMC010000026        33917 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.026, whole genome shotgun sequence.
ACCESSION   DAJTMC010000026 DAJTMC010000000
VERSION     DAJTMC010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33917
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.026"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            529..666
                     /locus_tag="OO130_001093"
     CDS             529..666
                     /locus_tag="OO130_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5364586.1"
                     /translation="MNVQAIFILMVVQLFGKEIAVTIEELQAVLVPHRLLSQLVKFRS
                     A"
     gene            1266..2045
                     /gene="ipaJ"
                     /locus_tag="OO130_001094"
     CDS             1266..2045
                     /gene="ipaJ"
                     /locus_tag="OO130_001094"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     protease IpaJ"
                     /protein_id="HCR5364587.1"
                     /translation="MSEQRKPCKRGCIHTGVMLYGVLLQGAIPREYMISHQTDVRVNE
                     NRVNEQGCFLARKQMYDNSCGAASLLCAAKELGVDKIPQYKGSMSEMTRKSSLDLDNR
                     CERDLYLITSGNYNPRIHKDNIADAGYSMPDKIVMATRLLGLNAYVVEESNIFSQVIS
                     FIYPDARDLLIGMGCNIVHQRDVLSSNQRVLEAVAVSFIGVPVGLHWVLCRPDGSYMD
                     PAVGENYSCFSTMELGARRSNSNFIGYTKIGISIVITNEAL"
     gene            complement(2989..3918)
                     /gene="virB"
                     /locus_tag="OO130_001095"
     CDS             complement(2989..3918)
                     /gene="virB"
                     /locus_tag="OO130_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence regulon transcriptional activator
                     VirB"
                     /protein_id="HCR5364588.1"
                     /translation="MVDLCNDLLSIKEGQKKEFTLHSGNKVSFIKAKIPHKRIQDLTF
                     VNQKTNVRDQESLTEESLADIIKTIKLQQFFPVIGREIDGRIEILDGTRRRASAIYAG
                     ADLEVLYSKEYISTLDARKLANDIQTAKEHSIRELGIGLNFLKVSGMSYKDIAKKENL
                     SRAKVTRAFQAASVPQEIISLFPIASELNFNDYKILFNYYKGLEKANESLSSTLPILK
                     EEIKDLDTNLPPDIYKKEILNIIKKSKNRKQNPSLKVDSLFISKDKRTYIKRKENKTN
                     RTLIFTLSKINKTVQREIDEAIRDIISRHLSSS"
     gene            complement(4135..4371)
                     /locus_tag="OO130_001096"
     CDS             complement(4135..4371)
                     /locus_tag="OO130_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR5364589.1"
                     /translation="MIKEKILSIVAFCYGIAYSKLSEETKFIEDLSADSLSLIEMLDM
                     ISFEFNLRIDESALEHIITIGDLISVVKNSTKSI"
     gene            complement(4375..6276)
                     /gene="ipaA"
                     /locus_tag="OO130_001097"
     CDS             complement(4375..6276)
                     /gene="ipaA"
                     /locus_tag="OO130_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector invasin IpaA"
                     /protein_id="HCR5364590.1"
                     /translation="MHNVNNTQAPTFLYKATSPSSTEYSELKSKISDIHSSQTSLKTP
                     ASVSEKENFATSFNQKCLDFLFSSSGKEDVLRSIYSNSMNAYAKSEILEFSNVLYSLV
                     HQNDLNFENEKGLQKIVAQYSELIIKDKLSQDSAFGPWSAKNKKLHQLRQNIEHRLAL
                     LAQQHTSGEALSLGQKLLNTEVSSFIKNNILAELKLSNETVSSLKLDDLVDAQAKLAF
                     DSLRNQRKNTIDSKGFGIGKLSRDLNTVAVFPELLRKVLNDILEDIKDSHPIQDGLPT
                     PPEDMPDGGPTPGANEKTSQPVIHYHINNDNRTYDNRVFDNRVYDNSYHENPENDAQS
                     PTSQTNDLLSRNGNSLLNPQRALVQKVTSVLPHSISDAVQTFANNSALEKVFNHTPDN
                     SDGIGSDLLTTSSQERSTNNSLSRGHRPLNIQNSSTTPPLHPEGVTSSNDNSSDTTKS
                     SASLSHRVASQINKFNSNTDSKVLQTDFLSRNGDTYLTRETIFEASKKVTNSLSNLIS
                     LIGTKSGTQERELQEKSKDITKSTTEHRINNKLKVTDANTINYVTETNADTIDKNHAI
                     YEKAKEVSSALSKVLSKIDDTSAELLTDDISDLKNNNDITAENNNIYKAAKDVTTSLS
                     KVLKNINKD"
     gene            complement(6285..7283)
                     /gene="ipaD"
                     /locus_tag="OO130_001098"
     CDS             complement(6285..7283)
                     /gene="ipaD"
                     /locus_tag="OO130_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle tip complex
                     protein IpaD"
                     /protein_id="HCR5364591.1"
                     /translation="MNITTLTNSISTSSFSPNNTNGSSTETVNSDIKTTTSSHPVSSL
                     TMLNDTLHNIRTTNQALKKDLSQKTLTKTSLEEIALHSSQISMDVNKSAQLLDILSKK
                     EYPINKDARELLHSAPKEAELDGYEMISHRELWDKIAKSINNINEQYLKVYEHAVSSY
                     TQMYQDFSAVLSSLAGWISPGGNDGNSVKLQVKSLKDELTKLKEKYKDKPLYPANNTV
                     SKEQANKWLTELGGTIGKVSEKNGGYVVNINMTPIDNMLKSLDNLGGNGEVVLDNAKY
                     QAWNAGFSAEDETMKNNLQTLVQKYSNANSIFDNLVKVLSSTISSCTDTDKLFLHF"
     gene            complement(7334..8425)
                     /gene="ipaC"
                     /locus_tag="OO130_001099"
     CDS             complement(7334..8425)
                     /gene="ipaC"
                     /locus_tag="OO130_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS translocon subunit IpaC"
                     /protein_id="HCR5364592.1"
                     /translation="MEIQNTKSAPILYTDISTKQTQSSSETQKSQNYQQLAAHIPLNV
                     GKNPVLTTTLNDDQLLKLSEQVQHDSEIIARLTDKKMKDLSEMSHTITPENTLDISSL
                     SSNAVSLIISVAVLLSALRTAETRLGSQLSLIAFDATKSAAENIVRQGLAALSSSITG
                     AVTQVGITGIGAKKTHSGISEQKGALRKNLATAQSLEKELAGSKLGLNKQIDTNITSP
                     QTNSSTKILGKNKLAPDNISLSTEHKTSLSSPDISLQDKIDTQRRAYELNTLSAQQKQ
                     NIGRATMETSAVAGNISTSGGRYTSALEEEEQLISQASSKQAEEASQVSKEASQATNQ
                     LIQKLLNIIDNINQSRNSTASQIAGNIRA"
     gene            complement(8445..10187)
                     /gene="ipaB"
                     /locus_tag="OO130_001100"
     CDS             complement(8445..10187)
                     /gene="ipaB"
                     /locus_tag="OO130_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system translocon subunit
                     IpaB"
                     /protein_id="HCR5364593.1"
                     /translation="MHNVSTTTTGLPLAKILASTELGDNTIQAANDAANKLFSLTIAD
                     LAANKNINTTNAHSTSNILIPELKAPKSLNASSQLTLLIGNLIQILGEKSLTALTNKI
                     TAWKSQQQARQQKNLEFSDKINTLLSETEGLTRDYEKQINKLKNADSKIKDLENKINQ
                     IQTRLSELAPDSPENKKLRREEIQLTIKKDAAVKDRTLIEQKTLSIHSKLTDKSMQLE
                     KEIDSFSAFSNTASAEQLSTQQKSLTGLASVTQLMATFIQLVGKNNEESLKNDLALFQ
                     SLQESRKTEMERKSDEYAAEVRKAEELNRVMGCVGKILGALLTIVSVVAAAFSGGASL
                     ALAAVGLALMVTDAIVQAATGNSFMEQALNPIMKAVIEPLIKLLSDAFTKMLEGLGVD
                     SKKAKMIGSILGAIAGALVLVAAVVLVATVGKQAAAKLAENIGKIIGKTLTDLIPKFL
                     KNFSSQLDDLITNAVARLNKFLGAAGDEVISKQIISTHLNQAVLLGESVNSATQAGGN
                     VASAVFQNNASKNLADLTLSKYQVEQLSKYIGDAIEKFGQLQEVIAELLASMSNSQAN
                     RTDVAKAILQQTTA"
     gene            complement(10193..10660)
                     /gene="ipgC"
                     /locus_tag="OO130_001101"
     CDS             complement(10193..10660)
                     /gene="ipgC"
                     /locus_tag="OO130_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SycD/LcrH family type III secretion system
                     chaperone IpgC"
                     /protein_id="HCR5364594.1"
                     /translation="MSLNITENESISTAVIDAINSGATLKDINAIPDDMMDDIYSYAY
                     DFYNKGRIEEAEVFFRFLCIYDFYNVDYIMGLAAIYQIKEQFQQAADLYAVAFALGKN
                     DYTPVFHTGQCQLRLKAPLKAKECFELVIQHSNDEKLKIKAQSYLDAIQDIKE"
     gene            complement(10718..11344)
                     /gene="ipgB1"
                     /locus_tag="OO130_001102"
     CDS             complement(10718..11344)
                     /gene="ipgB1"
                     /locus_tag="OO130_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB1"
                     /protein_id="HCR5364595.1"
                     /translation="MQILNKILPQVEFAIPRPSFDSLSHNKLVKKILSVFNLKQRFPQ
                     KNFGCPVNINKIRDSVIDKIKDSNSGNQLFCWMSQERTSYVSSMINRSIDEMAIHNGV
                     VLTSDNKKNIFAAIEKKFPDIKLDEKSAQTSISHTALNEIASSGLRAKILKRYSSDMD
                     LFNTQMKDLTNLVSSSVYDKIFNESTKVLQIEISAEVLKAVYRQSNTN"
     gene            complement(11360..11749)
                     /gene="ipgA"
                     /locus_tag="OO130_001103"
     CDS             complement(11360..11749)
                     /gene="ipgA"
                     /locus_tag="OO130_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgA"
                     /protein_id="HCR5364596.1"
                     /translation="MCRKLYDKLYEITGAKLDFNDKNQAFILLEEQIPVCITDNDEYI
                     FLTGLLNEHELFTENIINPEHILILNYSLSRDYGSSICLLPDTHQCVLTKKHYKKYLS
                     PDELIESLYEFLFCIKLTIANITSEVN"
     gene            complement(11762..13246)
                     /gene="icsB"
                     /locus_tag="OO130_001104"
     CDS             complement(11762..13246)
                     /gene="icsB"
                     /locus_tag="OO130_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector IcsB"
                     /protein_id="HCR5364597.1"
                     /translation="MILKISNFIDASNTKGPIRVEDTEHGPILVAQKFNLKDLFFRTL
                     STINAKINSQILNEQLKNYRLANQKSLLLFLKTLASEKSAESAFAAYEAVKNSIQHSF
                     TGKDIKLMLNTAESFHGIGTAKNLERHLVFRCWGNRGITHLGHTSISIKNNLLQEPTH
                     TYLSWYPGGNVTKDTEINYLFEKRSGYSVDTYKQDKLNMISDQTAERLDAGQEVRNLL
                     NSKQDQNNNKKIFFPRANQKKDPYGYWGVSADKVYIPLSGDNKTKDGKISYNLFGLDE
                     TNMSKFICQKKADAFRQLANYKLISKSENCAGMALNVLKAGNSEIYFPLPDVKLVATP
                     NNVYAYANKVRQRIESLNQSYNEIMKYIESDFDLSRLTQLRRSYLKSFNKINLIHTPK
                     TFKPLSISLYKHPTENVSSEDFDAVINACHSYLVKSAPSNMSRVLNELKTGATDKKEV
                     IIEKSIKTIDYYNSLKSPDLGTKLYIHDLLQVNKLLLNNSHSNI"
     gene            13560..15176
                     /locus_tag="OO130_001105"
     CDS             13560..15176
                     /locus_tag="OO130_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector inositol
                     phosphate phosphatase"
                     /protein_id="HCR5364598.1"
                     /translation="MHITNLGLHQVSFQSGDSYKGAEETGNQKGVSVISYQRVKNGER
                     NKGVEALNRLYLQNQTSLTGKGLLFARDRAAVFYEAIKLAGGDTSKIKAMMEQLDTYK
                     LGEVDKRNINELNKVISEEIRAQLGIKNKKELQSEIKQIFTNYLNNKNWEPINKNINY
                     NGKNYGFQLTPASHMKIGNKNIFVKEYNGKGICCASTRESKHIANMWLSKVVDDEGKE
                     IFSGIRHGVISAYGLKKNSSERAVAARNKAEELASAALYSRQELLSQALSGKTVDLKI
                     VSTSLLTPTSLTGGEESMLKDQVKALKELNSKRGEPTKLLIRNSDGLLQEVKVNLKVV
                     TFNFGVNELALKMGLGWRNVDKLNGESICSLLGDNFLKNGVIGGWAAEAIEKNPPCKN
                     DVIYLANQIKEILNKKLQKNDNGEPYKLSQRMALLAYTIGAVPCWNCKSGKDRTGMQD
                     AEIKREIIRKHETGQFSQLNSKLSSEEKRLFSTILMNSGNMEIQEMNTGVPGNKVMKK
                     LPLSSLELSYAERIGDPKIWNMVKGYSSFV"
     gene            15186..15548
                     /gene="ipgE"
                     /locus_tag="OO130_001106"
     CDS             15186..15548
                     /gene="ipgE"
                     /locus_tag="OO130_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgE"
                     /protein_id="HCR5364599.1"
                     /translation="MEDLADVICRALGIPSIDTDDQAIMLDDDVLIYIEKEGDSINLL
                     CPFCALPENINDLIYALSLNYSEKICLATDDEGGSLIARLDLTGINEFEDIYVNTEYY
                     ISRVRWLKDEFARRMKGY"
     gene            15548..16006
                     /gene="ipgF"
                     /locus_tag="OO130_001107"
     CDS             15548..16006
                     /gene="ipgF"
                     /locus_tag="OO130_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system lytic transglycosylase
                     IpgF"
                     /protein_id="HCR5364600.1"
                     /translation="MSRFVFIFLCFIPYLGRADCWDKAGERYNIPSSLLKAIAEKESG
                     FNKFAVNVNNNGSKDYGIMQINDFHSKRLREMGYTEEMLISHPCLSVHYAAKLLNEFM
                     LMYGRGWEAVGAYNAGTSPKKKKERLKYAEDIYKRYLRIAAESKQNNRRI"
     gene            16015..17130
                     /gene="mxiG"
                     /locus_tag="OO130_001108"
     CDS             16015..17130
                     /gene="mxiG"
                     /locus_tag="OO130_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiG"
                     /protein_id="HCR5364601.1"
                     /translation="MSEAKNSNLAPFRLLVKLTNGVGDEFPLYYGNNLIVLGRTIETL
                     EFGNDNFPENIIPVTDSKSDGIIYLAISKDNICQFSDEKGEQIDINSQFNSFEYDGVS
                     FQLKNMMEDKSRVHILNGMYNKPSVFFFFAVIVVLIIIFSLSLKKDEVKEIAEIIDDK
                     RYGIVNTGQCNYILAETQNDAVWASVALNKSGFTKCRYILVSNKEINRIQQYINQRFP
                     YINLYVLNLVSDKAELLVFLSKERNSSKDTELEKLKNALIVEFPYIKNIKFNYLSDHN
                     AREDAKGIFTKVNVQYKEICETNKVTYSVREELTDEKLELINRLISDHKNVYGDQYIE
                     FSVLLIDDDFKGKSYLNSKDSYVMLNDKHWFFLDKNK"
     gene            17138..17389
                     /locus_tag="OO130_001109"
     CDS             17138..17389
                     /locus_tag="OO130_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscF/YscF/HrpA family type III secretion system
                     needle major subunit"
                     /protein_id="HCR5364602.1"
                     /translation="MSVTVPDKDWTLSSLSETFDDGTQTLQGQLTSALNALAENPSNP
                     QLLAEYQSKLSEYTLYRNAQSNTVKVIKDVDAAIIQNFR"
     gene            17402..17695
                     /gene="mxiI"
                     /locus_tag="OO130_001110"
     CDS             17402..17695
                     /gene="mxiI"
                     /locus_tag="OO130_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod protein
                     MxiI"
                     /protein_id="HCR5364603.1"
                     /translation="MNYIYPVNQVDIIKASDFQSQEISSLEDVVSAKYSDIKMDTDIQ
                     VSQIMEMVSHPESLNPESLAKLQTTLSNYSIGVSLAGTLARKTVSAVETLLKS"
     gene            17701..18426
                     /gene="mxiJ"
                     /locus_tag="OO130_001111"
     CDS             17701..18426
                     /gene="mxiJ"
                     /locus_tag="OO130_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctJ family type III secretion inner membrane
                     ring lipoprotein Mxi"
                     /protein_id="HCR5364604.1"
                     /translation="MIRYKCFILFLLLMLIGCEQREELISNLSQRQANEIISVLERHN
                     ITARKVDGGKQGISVQVEKGTFASAVDLMRMYDLPNPERVDISQMFPTDSLVSSPRAE
                     KARLYSAIEQRLEQSLVSIGGVISAKIHVSYDLEEKNISSKPMHISVIAIYDSPKESE
                     LLVSNIKRFLKNTFSDVKYENISVILTPKEEYVYTNVQPVKEIKSEFLTNEVIYVFLG
                     MAVLVVILLVWAFKTGWFKRNKI"
     gene            18423..18950
                     /locus_tag="OO130_001112"
     CDS             18423..18950
                     /locus_tag="OO130_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HCR5364605.1"
                     /translation="MIRMDGIYKKYLSIIFDPAFYINRNRLNLPSELLENGVIRSEIN
                     NLIINKYDLNCDIEPLSGVTAMFVANWNLLPAVAYFIGSQESRLINHSEMVISYYGGK
                     ISKQGEAAIRSGFWHLIEWKENISVGIYERINLLFNPIALEGNYTPVERNLSRLNEGM
                     QYAKRHFTGIQTSCL"
     gene            18892..19587
                     /gene="mxiN"
                     /locus_tag="OO130_001113"
     CDS             18892..19587
                     /gene="mxiN"
                     /locus_tag="OO130_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mxi-Spa type III secretion system linker protein
                     MxiN"
                     /protein_id="HCR5364606.1"
                     /translation="MKVCNMQKGTLPVSRHHAYDGVVIKRIEKELCKTIKDRDTESKK
                     KAICVIKDATKKAESLRIDAVCDGYQIGIQTAFEHIIDYICEWKLKQNENRRNIEDYI
                     TSLLSENLHDERIISTLLEQWLSSLRNTVTELKVVLPKCNLALRKKLELDLHKYRSDV
                     KIILKYSEGNNYIFCSGNQVVEFSPQDVISGVKIELAEKLTKNDKKYFKELAHKKLRQ
                     IAEDLLKENPVND"
     gene            19580..19987
                     /gene="mxiM"
                     /locus_tag="OO130_001114"
     CDS             19580..19987
                     /gene="mxiM"
                     /locus_tag="OO130_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiM"
                     /protein_id="HCR5364607.1"
                     /translation="MINQINASNALQQRLNSEEFVNLNERLSSSQSFDENIIYEIMQY
                     FSQSELNSIDNDELHNKIEQLFNSRFPYLTAAQKSSLLNKLIDANQYVDLHEGFYASL
                     SIYNNIDFYIKTTTFDSLISVFEAGREADDSTW"
     gene            19971..20399
                     /gene="mxiM"
                     /locus_tag="OO130_001115"
     CDS             19971..20399
                     /gene="mxiM"
                     /locus_tag="OO130_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system pilotin MxiM"
                     /protein_id="HCR5364608.1"
                     /translation="MIRHGSNKLKIFILSILLLTLSGCALKSSSNSEKEWHIVPVSKD
                     YFSIPNDLLWSFNTTNKSINVYSKCISGKAVYSFNAGKFMGNFNVKEVDGCFMDAQKI
                     AIDKLFSMLKDGVVLKGNKINDTILIEKDGEVKLKLIRGI"
     gene            20529..21161
                     /gene="mxiE"
                     /locus_tag="OO130_001116"
     CDS             20529..21161
                     /gene="mxiE"
                     /locus_tag="OO130_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system transcriptional
                     activator MxiE"
                     /protein_id="HCR5364609.1"
                     /translation="MEGFFFVRNQNIKFSDNVNYHYRFNINSCAKFLAFWDYFSGALV
                     EHSHAEKCIHFYHENDLRDSCNTESMLDKLMLRFIFSSDQNVSNALAMIRMTESYHLV
                     LYLLRTIEKEKEVRIKSLTEHYGVSEAYFRSLCRKALGAKVKEQLNTWRLVNGLLDVF
                     LHNQTITSAAMNNGYASTSHFSNEIKTRLGFSARELSNITFLVKKINEKI"
     gene            21148..22848
                     /gene="mxiD"
                     /locus_tag="OO130_001117"
     CDS             21148..22848
                     /gene="mxiD"
                     /locus_tag="OO130_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system secretin MxiD"
                     /protein_id="HCR5364610.1"
                     /translation="MKKFNIKSLTLLIVLLPLIVNANNIDSHLLEQNDIAKYVAQSDT
                     VGSFFERFSALLNYPIVVSKQAAKKRISGEFDLSNPEEMLEKMTLLVGLIWYKDGNAL
                     YIYDSGELISKVILLENISLNYLIQYLKDANLYDHRYPIRGNISDKTFYISGPPALVE
                     LVANTATLLDKQVSSIGTDKVNFGVIKLKNTFVSDRTYNMRGEDIVIPGVATVVERLL
                     NNGKALSNRQAKNDPMPPFNITQKVSEDSNDFSFSSVTNSSILEDVSLIAYPETNSIL
                     VKGNDQQIQIIRDIITQLDVAKRHIELSLWIIDIDKSELNNLGVNWQGTASFGDSFGA
                     SFNMSSSASISTLDGNKFIASVMALNQKKKANVVSRPVILTQENIPAIFDNNRTFYVS
                     LVGERNSSLEHVTYGTLINVIPRFSSQGQIEMSLTIEDGTGNSQSNYNYNNENTSVLP
                     EVGRTKISTIARVPQGKSLLIGGYTHETNSNEIVSIPFLSSIPVIGNVFKYKTRNISN
                     IVRVFLIQPREIKESSYYNTAEYKSLISEREIQKTTQIIPSETTLLEDEKSLVSYLNY
                     "
     gene            22867..23934
                     /gene="mxiC"
                     /locus_tag="OO130_001118"
     CDS             22867..23934
                     /gene="mxiC"
                     /locus_tag="OO130_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system gatekeeper subunit
                     MxiC"
                     /protein_id="HCR5364611.1"
                     /translation="MLDVKNTGVFSSAFIDKLNAMTNSDDGDETADAELDSGLANSKY
                     IDSSDEMASALSSFINRRDLEKLKGTNSDSQERILDGEEDEINHKIFDLKRTLKDNLP
                     LDQDFIDRLKRYFKDPSDQVLALRELLNEKDLTAEQVESLTKIINEIISGSEKSVNAG
                     INSAIQAKLFGNKMKLEPQLLRACYRGFIMGNISTTDQYIEWLGNFGFNHRHTIVNFV
                     EQSLIVDMDSEKPSCNAYEFGFVLSKLIAIKMIRTSDVIFMKKLESSSLLKDGSLSAE
                     QLLLTLLYIFQYPSESEQILTSVIDVSRASHEDSVVYQTYLSSVNESPHDIFKSESER
                     EIAINILRELVTSAYKKELSR"
     gene            23947..26007
                     /gene="mxiA"
                     /locus_tag="OO130_001119"
                     /gene_synonym="virH"
     CDS             23947..26007
                     /gene="mxiA"
                     /locus_tag="OO130_001119"
                     /gene_synonym="virH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein MxiA"
                     /protein_id="HCR5364612.1"
                     /translation="MIQSFLKQVSTKPELIILVLMVMIIAMLIIPLPTYLVDFLIGLN
                     IVLAILVFMGSFYIERILSFSTFPSVLLITTLFRLALSISTSRLILVDADAGKIITTF
                     GQFVIGDSLAVGFVIFSIVTVVQFIVITKGSERVAEVAARFSLDGMPGKQMSIDADLK
                     AGIIDAAGAKERRSILERESQLYGSFDGAMKFIKGDAIAGIIIIFVNLIGGISVGMSQ
                     HGMSLSGALSTYTILTIGDGLVSQIPALLISISAGFIVTRVNGDSDNMGRNIMSQIFG
                     NPFVLIVTSALALAIGMLPGFPFFVFFLIAVTLTALFYYKKVVEKEKSLSESDSSGYT
                     GTFDIDNSHDSSLAMIENLDAISSETVPLILLFAENKISANDMEGLIERIRSQFFIDY
                     GVRLPTILYRTSNELKVDDIVLLINEVRADSFNIYFDKVCITDENGDIDALGIPVVST
                     SYNERVISWVDVSYTENLTNIDAKIKSAQDEFYHQLSQALLNNINEIFGIQETKNMLD
                     QFENRYPDLLKEVFRHVTIQRISEVLQRLLGENISVRNLKLIMESLALWAPREKDVIT
                     LVEHVRASLSRYICSKIAVSGEIKVVMLSGYIEDAIRKGIRQTSGGSFLNMDIEVSDE
                     VMETLAHALRELRNAKKNFVLLVSVDIRRFVKRLIDNRFKSILVISYAEIDEAYTINV
                     LKTI"
     gene            26020..26421
                     /gene="spa15"
                     /locus_tag="OO130_001120"
                     /gene_synonym="spaK"
     CDS             26020..26421
                     /gene="spa15"
                     /locus_tag="OO130_001120"
                     /gene_synonym="spaK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone Spa15/SpaK"
                     /protein_id="HCR5364613.1"
                     /translation="MSNINLVQLVRDSLFTIGCPPSIITDLDSHSAITISLDSMPAIN
                     IALVNEQVMLWANFDAPSDVKLQSSAYNILNLMLMNFSYSINELVELHRSDEYLQLRV
                     VIKDDYVHDGIVFAEILHEFYQRMEILNEVL"
     gene            26425..27717
                     /gene="spaL"
                     /locus_tag="OO130_001121"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
     CDS             26425..27717
                     /gene="spaL"
                     /locus_tag="OO130_001121"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system ATPase SpaL"
                     /protein_id="HCR5364614.1"
                     /translation="MSYIKLLTQLSFPNRISGPILETSLSDVSIGEICNIQAGIESNE
                     IIARAQVVGFHDEKTILSLIGNSRGLSRQTLIKPTAQFLHTQVGRGLLGAVVNPLGEV
                     TDKFAVTDNSEILYRPVDNAPPLYSERAAIEKPFLTGVKVIDSLLTCGEGQRMGIFAS
                     AGCGKTFLMNMLIEHSGADIYVIGLIGERGREVTETVDYLKNSEKKSRCVLVYATSDY
                     SSVDRCNAAYIATAIAEFFRTEGHKVALFIDSLTRYARALRDVALAAGESPARRGYPV
                     SVFDSLPRLLERPGKLKAGGSITAFYTVLLEDDDFADPLAEEVRSILDGHIYLSRNLA
                     QKGQFPAIDSLKSISRVFTQVVDEKHRIMATAFRELLSEIEELRTIIDFGEYKPGENA
                     SQDKIYNKISVVESFLKQDYRLGFTYEQTMELLGETIR"
     gene            27810..28148
                     /gene="spa13"
                     /locus_tag="OO130_001122"
                     /gene_synonym="spaM"
     CDS             27810..28148
                     /gene="spa13"
                     /locus_tag="OO130_001122"
                     /gene_synonym="spaM"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858283.1"
                     /note="Not similar to SpaM from Salmonella T3SS; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein Spa13"
                     /protein_id="HCR5364615.1"
                     /translation="MEALDKRIIYFLQLENDLEPVGAQSVSQLFNTRRKIAIVKKHII
                     QYQSERILLKGRIEEIQKDIDEANASKRKLLHKESKICKRIGLIKRNNFAKQLILDEL
                     SQEDMKYGIR"
     gene            28135..29013
                     /gene="spa32"
                     /locus_tag="OO130_001123"
     CDS             28135..29013
                     /gene="spa32"
                     /locus_tag="OO130_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle length
                     regulator Spa32"
                     /protein_id="HCR5364616.1"
                     /translation="MALDNINLNFSSDKQIEKCEKLSSIDNIDSLVLKKKRKVEIPEY
                     SLIASNYFTIDKHFEHKHDKGEIYSGIKNAFELRNERATYSDIPESMAIKENILIPDQ
                     DIKAREKINIGDMRGIFSYNKSGNADKNFERSHTSSVNPDNLLESDNRNGQIGLKNHS
                     LSIDKNIADIISLLNGSVAKSFELPVMNKNTADITPSMSLQEKSIVENDKNVFQKNSE
                     MTYHFKQWGAGHSVSISVESGSFVLKPSDQFVGNKLDLILKQDAEGNYRFDSSQHNKG
                     NKNNSTGYNEQSEEEC"
     gene            29007..29888
                     /gene="spa33"
                     /locus_tag="OO130_001124"
                     /gene_synonym="spaO"
     CDS             29007..29888
                     /gene="spa33"
                     /locus_tag="OO130_001124"
                     /gene_synonym="spaO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctQ family type III secretion system ring
                     protein Spa33"
                     /protein_id="HCR5364617.1"
                     /translation="MLRIKHFDANEKLQILYAKQLCERFSIQTFKNKFTGSESLVTLT
                     SVCGDWVIRIDTLSFLKKKYEVFSGFSTQESLLHLSKCVFIESSSVFSIPELSDKITF
                     RITNEIQYATTGSHLCCFSSSLGIIYFDKMPVLRNQVSLDLLHHLLEFCLGSSNVRLA
                     TLKRIRTGDIIIVQKLYNLLLCNQVIIGDYIVNDNNEAKINLSESNGESDHTEVSLAL
                     FNYDDINVKVDFILLEKKMTINELKMYVENELFKFPDDIVKHVNIKVNGSLVGHGELV
                     SIEDGYGIEISSWMVKE"
     gene            29893..30543
                     /gene="spa24"
                     /locus_tag="OO130_001125"
                     /gene_synonym="spaP"
     CDS             29893..30543
                     /gene="spa24"
                     /locus_tag="OO130_001125"
                     /gene_synonym="spaP"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa24/SpaP"
                     /protein_id="HCR5364618.1"
                     /translation="MLSDMSLIATLSFFTLLPFLVAAGTCYIKFSIVFVMVRNALGLQ
                     QVPSNMTLNGIALIMALFVMKPIIEAGYENYLNGPQKFDTISDIVRFSDSGLIEYKQY
                     LKKHTDLELARFFQRSEEENADLKSAENNDYSLFSLLPAYALSEIKDAFKIGFYLYLP
                     FIVVDLVISSILLALGMMMMSPITISVPIKLVLFVALDGWGILSKALIEQYINVPA"
     gene            30558..30818
                     /gene="spaQ"
                     /locus_tag="OO130_001126"
     CDS             30558..30818
                     /gene="spaQ"
                     /locus_tag="OO130_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein SpaQ"
                     /protein_id="HCR5364619.1"
                     /translation="MSDIVYMGNKALYLILIFSLWPVGIATVIGLTIGLLQTVTQLQE
                     QTLPFGIKLIGVSISLLLLSGWYGEVLLSFCHEIMFLIKSGV"
     gene            30818..31588
                     /gene="spa29"
                     /locus_tag="OO130_001127"
                     /gene_synonym="spaR"
     CDS             30818..31588
                     /gene="spa29"
                     /locus_tag="OO130_001127"
                     /gene_synonym="spaR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa29/SpaR"
                     /protein_id="HCR5364620.1"
                     /translation="MDISSWFESIHVFLILLNGVFFRLAPLFFFLPFLNNGIISPSIR
                     IPVIFLVASGLITSGKVDIGSSVFEHVYFLMFKEIIVGLLLSFCLSLPFWIFHAVGSI
                     IDNQRGATLSSSIDPANGVDTSELAKFFNLFSAVVFLYSGGMVFILESIQLSYNICPL
                     FSQCSFRVSNILTFLTLLASQAVILASPVMIVLLLSEVLLGVLSRFAPQMNAFSVSLT
                     IKSLLAIFIIFICSSTIYFSKVQFFLGEHKFFTNLFVR"
     gene            31595..32623
                     /gene="spa40"
                     /locus_tag="OO130_001128"
                     /gene_synonym="spaS"
     CDS             31595..32623
                     /gene="spa40"
                     /locus_tag="OO130_001128"
                     /gene_synonym="spaS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     switch protein Spa40/SpaS"
                     /protein_id="HCR5364621.1"
                     /translation="MANKTEKPTPKKLKDAAKKGQSFKFKDLTTVVIILVGTFTIISF
                     FSLSDVMLLYRYVIINDFEINEGKYFFAVVIVFFKIIGFPLFFCVLSAVLPTLVQTKF
                     VLATKAIKIDFSVLNPVKGLKKIFSIKTIKEFFKSILLLIILALTTYFFWINDRKIIF
                     SQVFSSVDGLYLIWGGLFKDIILFFLAFSIFVIILDFVIEFILYMKDMMMDKQEIKRE
                     YIEQEGHFETKSRRRELHIEILSEQTKSDIRNSKLVVMNPTHIAIGIYFNPEIAPAPF
                     ISLIETNQCALAVRKYANEVGIPTVRDVKLARKLYKTHTKYSFVDFEHLDEVLRLIVW
                     LEQVENTH"
     gene            32636..32878
                     /locus_tag="OO130_001129"
     CDS             32636..32878
                     /locus_tag="OO130_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5364622.1"
                     /translation="MCYMGVNFCNKIGIDQSEFEIESSIINSIANEVLNPISFLSNKD
                     IINVLLRKISSECDLVRKDIYRCALELVVEKTPDDL"
     gene            complement(33485..33917)
                     /locus_tag="OO130_001130"
                     /pseudo
     CDS             complement(33485..33917)
                     /locus_tag="OO130_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT        10563 a         4966 c         6796 g        11592 t
ORIGIN      
        1 ttgttgactg cctgtgcggc atctggtatc aaggtggcgt gaagccggtt caggttctca
       61 tgcggaacgt tggaacctat atccccccga taccaaaggc gaaatgtaag tgacaaaatg
      121 caatgaacac gttctccgtt aactgaggag catggtgata aataaaggta aatgcagaag
      181 gtgtagagtc tcgcagattt ctgacatcat agagcctaac attagatgca taacgctaca
      241 gattgtagaa tctctacagg tgaaatatac actggttggc gtttatgtcg caaaacatgt
      301 cattcaggtt catctcacca ataaggatat gagtgaagtg gaggataagt gagtctgctg
      361 tcagagtttt ctggtgtatg tcagtaactg cgaaccatgt ctgcttgata tgggagctta
      421 tggaggctct ctgtacaagg ttagggagct aacaaagcag ggataaaaag tccgcctgat
      481 gcaggcacgg ttcgttaaat cttttgccat tggtaacaaa atggtgcgat gaatgttcag
      541 gctatcttta tcttgatggt ggttcagctg tttggtaaag aaatcgctgt aacaatagaa
      601 gaactgcagg cagtactggt cccacatcgt ttgctcagtc aactggtgaa gttccgtagc
      661 gcgtaaatta atgctttgca tgaaacgtta ctggagtgga tgaaagaata cctgcatata
      721 tcattactgc gttggattac cagtacaaca gattgaatga actggacatg ttgatagacg
      781 gcattgaaaa gcagcttata agcatagtgc ggcagagtta attggagagc acttcgcctt
      841 ctgagtatta agtaatcatg gtggtacctg cggatactat ctattcactg tgctcggtcg
      901 gcagccttac tgaccaagga gttgggggag ttgataacaa agttgaaaaa acagctctca
      961 accaaggtgg ttattgtaac aatagcaaat aagctggtgt gacctgtatc acaataaccg
     1021 attatggacg taaatacgac aaaaatatgt cagtatcagg cggtattaat tgctgatgct
     1081 gatggacatt aactcttaat aaaagggtgg atgctgaaag gttgttatgg tgacggacgt
     1141 gatgacgacg gaaaagagta tgaggttttg gatttaaagt tcgttcgggg aataaggacc
     1201 cggtcagcta attctcatag tagttcgcat tgctggatgc aatactgtgc tgcaattact
     1261 cgtcgatgtc ggaacaacgg aagccttgca aacggggatg tattcataca ggtgttatgt
     1321 tatatggggt tttattgcag ggggctatac caagagaata tatgatatct catcagactg
     1381 atgtgagggt aaatgagaat agagtaaacg aacaaggttg ttttttggcg aggaagcaga
     1441 tgtatgacaa ttcttgtgga gctgccagtt tactctgtgc tgcaaaggaa ttaggggttg
     1501 ataaaatacc acaatacaaa gggtctatgt ccgaaatgac aaggaagagt tctcttgatt
     1561 tggataatcg ttgtgagcgg gatttatact taataactag tggtaattat aatccgagaa
     1621 tacacaaaga taatatcgcg gatgccggtt attctatgcc tgataagata gtcatggcta
     1681 cgaggcttct tggattgaat gcttatgtcg ttgaagaaag taacattttt tcgcaagtaa
     1741 ttagttttat ttatcccgat gcaagggact tattaattgg catgggatgt aatatagtac
     1801 atcaacgaga tgtgttatct tctaaccaaa gagttttgga agctgttgct gtatccttta
     1861 ttggagttcc tgtcggcctc cactgggtgt tgtgtagacc tgatggaagc tatatggatc
     1921 cagctgtagg agaaaattat tcttgttttt caactatgga attaggtgca agaagaagta
     1981 attctaactt tataggatat actaaaatag gtatttccat agttataact aatgaggctt
     2041 tgtaaattgt cgttttttat ctgtgaagga gcttgaatat tttggggcgg gggcataaca
     2101 aatgatatta ctgagtacaa atttagctat attgggcgga ttagttgtgt gggaagacga
     2161 aatgtttctc ttgaaataag tgaactccct caaggaatga ttgtgtgctt aaatatttaa
     2221 gtgctggtaa gtttgagaag gctgacatat tttgcagagc aaacagtgat tgacttggat
     2281 gatattttta ttttagtcac tcgttgtaat tggagtatgc tttattttat ttgaaaatag
     2341 atgtatttta tacccgcaat ttaaactgta agccctatga ggggtatgga tattataagt
     2401 tactctttga ctaagcataa cttttggggt tagaaaaata aatctcttgt taataattaa
     2461 tatgatttta aaatcaaact tgtttataat tataaaatca tgtaagtcgg tcattttatg
     2521 ggaagattag gagaataaaa cagtaattat atttgagaaa tctgctaata tattcttaaa
     2581 gcttttttga tactgtgaac gaagtctgtt aatggtggat tttttgttta tcaccatgta
     2641 aaaaattgag tagtaaaagc gttgactgct aatcctcgct gtttttattg aaacgattaa
     2701 aaatacgctg aatattttcg gccagtcact catcttcaca taaaattttg accatcatgc
     2761 cgcatccctt agttgcacaa ttcattttca atgatatgaa tattactaat ttcaccagat
     2821 aatgttctcg tggtcagcag ggtatgagtt ttcaccgtaa ttttaaggaa acacagtgcg
     2881 tggggattga ataacagata gccgacggaa ttaccccacc ggctgaaagt caagttcaac
     2941 gaagattttt attatctgaa ttgggcagtt tacatcagtg ttcgatgttt atgaagacga
     3001 tagatggcga gaaattatat ctcgaatagc ttcatctatt tctctctgaa ctgttttgtt
     3061 tattttagat aatgtaaata ttagagttct gtttgtttta ttctcttttc tttttatgta
     3121 agtccgtttg tctttagaaa taaataacga gtcaactttt agcgaagggt tttgctttct
     3181 gtttttgctt ttctttatga tgtttaaaat ttctttttta tatatgtccg ggggcaaatt
     3241 tgtatcaagg tcttttattt cttcctttaa tattggtagt gtagaactaa gagattcatt
     3301 agccttttca agtcctttat aataattgaa taatatcttg tagtcattaa agttaagttc
     3361 tgacgcgatt ggaaatagag atattatttc ctgtggaacg cttgctgcct gaaaggcacg
     3421 agtgactttc gcgcgagaca gattctcttt tttggctatg tctttatagg acatccctga
     3481 tactttcaga aaattaagac caataccaag ttctcggatg ctatgctctt ttgctgtttg
     3541 tatatcgttt gctagttttc tggcatcaag agtagatata tattcttttg aatatagaac
     3601 ttcaagatct gctcctgcat atattgcaga tgctcttcta cgagtgccat ccagaatttc
     3661 aattctacca tcaatctccc ttcctattac agggaagaat tgttgtagct ttatagtttt
     3721 tatgatatcg gctaatgatt cttctgttag ggattcttga tcgcgtacat tcgttttttg
     3781 gttgacgaag gttaaatctt ggatcctttt atgaggaatc ttggctttga taaaggaaac
     3841 tttattacca gaatggagtg taaactcttt cttttggcct tcctttatac ttaacaagtc
     3901 gttgcacaaa tccaccatat cacaccctgt ttattcatat tgatttatat tgacaaaagt
     3961 taaatgcagt gtgagcaaga ttgtatgtaa ctgttgatga tagtcaacgg tagtcttaaa
     4021 aattactatt ttaatagctg gattgatgca atttatcttt attggataaa agaataatat
     4081 tcttgtggag ctctgatgtg agagctccat agatgaaaaa atgcttatca attactatat
     4141 tgattttgta ctatttttga ctacagaaat aagatctcca atagtaataa tgtgttctaa
     4201 tgcagattca tctatacgta gattgaactc aaaactaatc atatccagca tttcaatcag
     4261 agaaagagaa tcggcagaaa gatcttcaat aaatttagtt tcttcagata gtttgctgta
     4321 cgctatacca tagcaaaagg ccactattga taatattttt tcttttatca tattttaatc
     4381 cttattgata ttctttaata cttttgatag ggaagtggtt acatcttttg ctgctttata
     4441 tatattattg ttttcagctg taatatcatt gttatttttt aaatcagata tatcatctgt
     4501 aagtaattct gcagaggtat cgtcaatttt tgacaatacc ttgctgaggg cgctagatac
     4561 ttcttttgcc ttttcataga tcgcatgatt tttatcaatt gtatctgcgt tggtttctgt
     4621 tacgtagttt attgtatttg catctgtaac ttttaatttg ttgtttattc tgtgttctgt
     4681 tgtggatttt gtaatgtcct ttgatttttc ctgtaactct cgttcttgtg ttcctgattt
     4741 agttccaatg agagatataa gattacttag ggagtttgtt actttttttg aagcttcaaa
     4801 tatcgtttcc cgtgttaaat atgtgtctcc atttcttgat aaaaaatcag tctgaagtac
     4861 ttttgaatca gtgtttgagt tgaatttatt gatttgcgaa gctactctat gagaaagaga
     4921 agcgctactt ttagttgtat ctgatgagtt atcattactg cttgtcactc cttccgggtg
     4981 gagagggggg gtggttgaag agttctgtat gttcagaggc ctgtgtcccc gagaaagaga
     5041 gttatttgta gatctttctt gactactcgt agttaacagg tctgaaccta ttccatccga
     5101 attatctgga gtatggttga aaaccttttc taaagctgaa ttatttgcaa atgtctggac
     5161 agcatctgat atagagtgtg gtagaacgga agttactttt tgaactagtg ctctttgtgg
     5221 atttagtaat gagtttccgt tacgggataa tagatcgttt gtctgagaag taggagactg
     5281 tgcatcattt tctgggttct cgtgatagct attgtcatat actctgttgt caaaaactct
     5341 attatcgtaa gttctattat cattatttat atgatagtga attacaggtt gggatgtttt
     5401 ctcattggct ccgggggttg gtccgccatc tggcatatct tcgggaggtg tagggaggcc
     5461 atcttggata ggatgcgaat cttttatatc ttctaaaata tcattaagga cttttctcaa
     5521 cagctcagga aacacggcta ctgtatttaa gtctcttgac agtttaccta taccaaatcc
     5581 tttactatca atagtatttt tacgttgatt gcgcaaacta tcaaaggcaa gttttgcctg
     5641 tgcgtcaact aaatcatcta gtttgagaga tgaaacagtt tcattactta actttaattc
     5701 agcaagaata ttattcttga taaatgatga tacttcagta ttgaggagtt tttgtcccaa
     5761 tgataaagct tcaccagatg tgtgttgttg tgctaatagt gcaagtctgt gctcaatgtt
     5821 ttgtcgtaat tgatggagtt tcttattctt tgccgaccat ggtccaaagg cagaatcttg
     5881 ggataattta tcttttataa ttagttccga atactgtgcg acaatttttt gaagtccctt
     5941 ttcgttttca aaattaagat cattttgatg tactaaggag tacaaaacat ttgaaaattc
     6001 gagaatctcg cttttggcat acgcattcat tgagttggaa taaatgcttc ttaacacatc
     6061 ttctttccct gaggaagaaa ataaaaaatc aagacatttc tgattaaaag acgttgcaaa
     6121 gttttctttt tcagacactg atgctggtgt ttttagagaa gtttgcgaac tatggatatc
     6181 ggatattttg ctttttaact cgctgtattc tgttgatgat ggtgaagttg ccttatataa
     6241 gaatgttggc gcttgagtat tatttacatt atgcatggcg cacctcagaa atggagaaaa
     6301 agtttatctg tatctgtaca tgagcttatt gtactactca aaacctttac taaattatca
     6361 aaaatactat tggcattact gtatttttga actaaagttt gaagattatt tttcattgtt
     6421 tcatcttcgg cagagaatcc ggcattccat gcctgatatt ttgcattatc tagcacaacc
     6481 tcgccatttc cacctagatt atctaagctt tttaacatat tgtctattgg ggtcatgttt
     6541 atattgacaa catatccccc gtttttttca gataccttgc cgattgttcc acctaattct
     6601 gtaagccatt tatttgcttg ttccttacta acagtattat ttgctggata tagcggttta
     6661 tctttatatt tttccttgag ctttgtcaat tcgtctttaa gcgatttgac ttgtaatttc
     6721 acggagtttc cgtcgttacc tccgggagag atccagccgg caagactgga aagaacagcg
     6781 ctaaaatctt gatacatttg agtatatgaa ctaacggcat gttcatatac tttcagatac
     6841 tgttcattaa tattattgat tgactttgca attttatccc acagttctct gtgagatatc
     6901 atttcatatc catcaagctc ggcttctttc ggggctgaat gtaataattc tcttgcgtct
     6961 ttattaattg gatattcttt cttggaaaga atatccaata gttgagcgga tttgtttaca
     7021 tccatgctaa tctgagatga atgtaatgct atttcttcta gcgatgtttt agtcaacgtt
     7081 ttttgtgaga gatctttctt taatgcctga tttgttgttc tgatattatg aagggtgtcg
     7141 ttgagcatag taagggaact tacaggatga gaactggtcg ttgtttttat atcagaatta
     7201 actgtttcgg ttgatgaacc gttggtattg tttgggctga atgatgaggt ggaaatacta
     7261 ttagtcagag ttgttatatt catggttatt tccttatagg aagagccata tattattatg
     7321 gctcttggat atattaagct cgaatgttac cagcaatctg actggctgta gaattccttg
     7381 attggttgat gttgtcaatt atattcaata atttttgtat taattgattt gtcgcttggg
     7441 atgcttcttt agatacttgg gatgcttcct ctgcttgttt actgctggcc tgactgatta
     7501 gttgttcttc ttcttcaaga gcagatgtat aacgccctcc tgatgtggat atattaccag
     7561 caacggctga tgtctccatt gttgcacggc caatgttttg tttttgctga gcagaaaggg
     7621 tattgagctc gtaagctctt ctctgggtgt caattttatc ctgcaaagaa atatcgggag
     7681 aactaagaga agttttatgt tccgttgaca gggatatatt atctggtgcc agtttatttt
     7741 tacctaaaat ttttgtgcta gagttagttt gtggtgaggt gatatttgta tctatttgtt
     7801 tatttaaccc taatttagaa cctgcaagct ctttttcaag agattgagca gtggcaaggt
     7861 tctttcttaa ggctcctttt tgttcgctaa tccctgaatg cgtttttttg gcaccgatac
     7921 ccgttatacc tacttgtgtg accgctccag taatgcttga tgatagggct gctaggcctt
     7981 gccgaacaat gttctctgca gctgattttg tagcatcgaa cgcaatcaat gacaattgag
     8041 agcccaatct agtttctgca gtgcggagag cagaaagtag aacggctaca ctaataatta
     8101 aagaaacagc attagaagaa agactggaaa tatccagggt gttctctggg gtaattgtgt
     8161 gactcatttc tgaaagatct ttcatctttt tgtcagtaag gcgagcaatg atttctgaat
     8221 catgctgaac ctgctctgat aactttaaaa gttgatcatc atttaatgta gttgttaata
     8281 cgggattttt accgacatta agtggaatat gtgctgctaa ctgctgataa ttttgtgatt
     8341 tttgtgtttc ggaagaactt tgagtttgtt ttgtagatat atctgtatat aaaatcgggg
     8401 ctgattttgt gttttgaatt tccataacat tctccttatt tgtatcaagc agtagtttgt
     8461 tgcaaaattg cttttgcaac atcagttcta ttagcctgag agttggacat tgaggctaac
     8521 agctctgcaa ttacttcctg caattggccg aatttttcta ttgcgtcacc gatgtatttt
     8581 gacagttgtt caacttgata tttcgataat gtcaggtctg ctagattttt cgacgcgttg
     8641 ttttggaaaa cagcagaagc gacatttcct cccgcttgtg tggcagagtt aacactttct
     8701 cctaataaaa ctgcttggtt taaatgggtg gaaataattt gtttggatat tacttcatca
     8761 cccgctgcac caagaaattt atttaatctg gcaacagcat tagtgattaa atcgtccagt
     8821 tgagaagaaa aattcttgag aaactttggt ataaggtctg tgagggtttt acctattatt
     8881 ttgccaatat tctctgcaag ttttgctgct gcctgtttac caacagtggc tacgagaacg
     8941 actgctgcaa ccaagacaag agcgcctgcg attgccccca gaatagagcc aatcattttg
     9001 gcttttttcg agtcgacgcc caagccttcg agcatttttg taaatgcatc tgaaaggagt
     9061 ttgattaagg gttcaatgac tgctttcatg atcggattca gggcttgttc catgaaggaa
     9121 ttgccggtcg ctgcttgtac tatagcatcc gtaaccataa gagctaaacc aacagctgcc
     9181 agtgctagag atgctcctcc agaaaaagct gctgcaacaa cactaacgat agttaaaagt
     9241 gccccaagta ttttcccaac acaacccatt actctgttga gttcttctgc tttacgtact
     9301 tcagcagcat actcatcaga ttttctctcc atttcagttt ttcttgattc ttggagagac
     9361 tggaatagag ccagatcatt ttttaaagat tcttcattat tttttccaac tagttgaata
     9421 aaggttgcca tcaattgagt aacactggca agtccggtta atgatttctg ctgggttgat
     9481 agctgttcag cagatgctgt gtttgaaaat gcagaaaaag agtctatttc tttttcgagt
     9541 tgcattgatt tatctgtaag tttgctatga attgacagag ttttctgctc aatcaatgtc
     9601 ctgtctttaa ctgctgcgtc ttttttgata gtgagttgta tttcttcccg tcttaatttt
     9661 ttattttctg gtgaatctgg ggcgagttcg gataatcttg tttgaatttg gttaatttta
     9721 ttttctaggt cttttatttt tgaatctgcg ttttttagtt tattaatttg tttttcatag
     9781 tctctggtta gtccttcagt ttcagataga agagtattaa ttttatcgga gaattctagg
     9841 tttttttgct gtcttgcctg ttgctgggac ttccaggcag taattttatt tgttaatgca
     9901 gttaaagatt tttcaccaag tatttgaata aggtttccaa ttaaaagcgt tagttgggaa
     9961 cttgcattta atgactttgg tgctttaagt tcagggatta atatattcga agttgagtgt
    10021 gcattagttg tattaatatt tttgttagca gcaagatcag caattgtaag agaaaataat
    10081 ttgttagctg catcatttgc agcctggata gtattgtctc caagctcagt ggaagccaat
    10141 attttggcaa gaggcaaacc agtggttgtg gtgcttacat tatgcataat aattactcct
    10201 tgatatcctg aattgcgtct aagtatgatt gtgcttttat ttttaatttt tcatcattgc
    10261 tgtgttgaat tacgagttcg aagcactctt tggctttcaa gggggctttc aaccgaagct
    10321 ggcattgtcc agtatggaat accggtgtat agtcattttt tcctaatgca aaagcgacag
    10381 cataaaggtc tgctgcttgt tggaactgtt cttttatctg ataaatagct gcgagtccca
    10441 taatgtagtc tacattgtaa aagtcgtata tacataaaaa tctgaagaaa acttcagctt
    10501 cctctattct tcctttgttg taaaagtcat aagcatatga ataaatgtca tccatcatat
    10561 catcaggaat tgcattaata tctttcagtg tagcgccaga gttaattgca tcaattactg
    10621 cagtagagat gctttcattt tcagtgatat ttaaagacat aaggtctcct ttttgtctgc
    10681 attactttaa taagtataag atttaatata aacagattta atttgtattg ctttgacggt
    10741 atacagcctt tagaacctct gcgcttattt ctatttgtaa aacttttgtt gattcattaa
    10801 atattttatc gtagacggaa ctggatacta gatttgtaag atccttcatt tgtgtgttaa
    10861 acaaatccat gtcactgcta taacgtttta aaatctttgc tctgaggcca gatgaggcaa
    10921 tctcgtttag tgctgtatga ctgatagatg tttgtgctga tttttcatca agttttatat
    10981 ccgggaattt tttttcaata gctgcaaata tgtttttttt gttatctgag gtcaaaacaa
    11041 ctccattgtg tatggccatt tcatctatac ttctgtttat cattgaagag acataagagg
    11101 ttcgctcttg gctcatccaa caaaatagtt gattccccga attactatct tttattttgt
    11161 ctatgacgct gtccctgatt ttatttatat tcaccgggca tccaaaattc ttctgaggaa
    11221 agcgttgttt taagttaaaa acagataata ttttctttac aagcttattg tgacttaacg
    11281 aatcaaatga tgggcgagga atcgcgaatt caacctgtgg aagtattttg tttagaattt
    11341 gcatgatacc ccctatatgt tagttcactt ctgaagtgat gtttgcaatg gttagtttaa
    11401 tacaaaataa aaattcgtac aaagattcaa tgagttcatc aggtgaaaga tattttttgt
    11461 agtgtttctt ggtcaaaaca cattgatgag tatcagggag aagacagata gaagaaccat
    11521 aatctctact caaagagtag ttaaggatga gtatgtgttc tggattaata atattttctg
    11581 taaaaagttc atgttcatta agaagtccgg tcaaaaagat gtattcatca ttatctgtta
    11641 tacaaacagg tatttgttct tcgagcaata taaaggcttg atttttatcg ttaaaatcaa
    11701 gctttgcacc tgttatttca taaagtttat catatagttt gcgacacatt tctatggcct
    11761 tctatatatt agaatgagag ttattcaata aaagcttatt gacctgtaat aagtcgtgaa
    11821 tgtaaagttt ggttccaaga tctggcgatt taagagaatt gtaataatca atagttttta
    11881 ttgacttttc gataattact tcttttttat ctgttgctcc ggtttttaat tcgtttaata
    11941 cacgagacat attgctgggt gctgatttta ctaagtatga gtggcaagcg ttgatgaccg
    12001 catcaaagtc ttctgaagat acattttcag taggatgttt gtaaagtgaa attgataaag
    12061 gcttgaatgt ttttggggtg tggataaggt taattttatt gaaactttta agatagctac
    12121 gtcgtaattg agtcaatctg gaaagatcaa aatcagattc aatatacttc attatctcgt
    12181 tataagactg attgagactt tcaatgcgtt gcctaacttt atttgcatac gcgtatacat
    12241 tgttaggtgt agcaaccaat ttaacgtcag ggagtggaaa gtatatttca gaatttcctg
    12301 ctttaagaac attaagtgcc ataccagcac agttttcaga tttactgatt aatttataat
    12361 ttgccaattg tctgaaagca tctgctttct tttggcatat aaattttgac atgtttgttt
    12421 catcaaggcc aaaaaggtta tagctgattt taccatcctt tgttttatta tcacctgata
    12481 atggaatgta tactttatca gcactgaccc cccaataacc ataggggtct tttttttgat
    12541 tagctctggg aaaaaatatt tttttgttgt tattttgatc ttgttttgaa tttaataagt
    12601 ttctaacctc ttggcctgca tcaagtcttt cggctgtttg gtctgaaatc atatttagtt
    12661 tgtcttgttt ataggtatcg acactgtaac ctgagcgttt ttcaaaaagg tagtttattt
    12721 ctgtatcttt tgtaacattc ccgccgggat accagctgag ataggtatga gttggttcct
    12781 gtagtaaatt gttttttata ctgatactgg tatgccctaa atgggttatg cctctatttc
    12841 cccagcaacg aaacactaaa tgtctttcaa gatttttggc tgtaccgatg ccatgaaaac
    12901 tctctgcggt attcaacatt aatttgatgt ctttacctgt aaaagaatgt tgaattgaat
    12961 ttttgactgc ttcataggcg gcaaacgcac tttctgcaga tttttcgcta gctaatgtct
    13021 tgaggaaaag caataaggat ttttgattcg ctagtcgata attttttagt tgttcattta
    13081 atatctgtga atttatcttt gcattaatag tgcttaatgt tctgaaaaag agatccttta
    13141 ggttgaattt ctgtgctacc aatataggtc catgctctgt atcttcaacg cgtataggcc
    13201 cttttgtatt gcttgcgtca atgaaattgc taattttgag aatcatactt tattaactct
    13261 ccattacgtg gtgattgtgg ggatgtaaaa tgtatttgat atataatata ttcaattaat
    13321 aaatttagaa acttgagcct gttaacataa tcaaattttc ttttgctgta cataatatgt
    13381 acctcgtgag catatgtagt gctggtttca tcatgaaatc ccacaagtta aagtgtctga
    13441 tatatcaggc tcggagtgtt atagaaaaag agagaaccct gttgaataag tatctttttt
    13501 gttgtaaaac gaggtacaaa actgcaatca atgccccatt tatgactgag gataattaaa
    13561 tgcacataac taatttggga ttgcatcagg tttcatttca aagcggagat tcctataaag
    13621 gcgcagaaga aaccggaaac caaaaaggtg taagcgtgat ttcatatcaa agagttaaaa
    13681 atggagagag aaataagggg gtcgaagcgc ttaatcgatt atatttacag aaccaaacaa
    13741 gcttaacagg gaaaggcctt ttgtttgcgc gtgacagagc tgcagttttt tacgaggcaa
    13801 taaagttggc aggtggtgat acgtcaaaaa ttaaagccat gatggaacaa ttagatacct
    13861 ataaacttgg tgaagttgat aaaagaaata ttaatgagct taataaagta ataagtgaag
    13921 agataagagc acagctaggc attaaaaata aaaaggaatt gcaatcggaa ataaaacaaa
    13981 tatttactaa ttatttaaat aataagaatt gggaaccgat caacaaaaat attaattata
    14041 atgggaaaaa ttatggtttt caattaacac ccgcctctca tatgaagata gggaataaaa
    14101 atatatttgt caaagagtat aatggaaaag gaatttgttg tgcttctacg agagagagta
    14161 agcatattgc gaatatgtgg ctttcaaaag tggtggatga cgaggggaag gaaatttttt
    14221 cgggtatcag acatggagtt atatcagcct atggattgaa aaaaaactca tctgaaagag
    14281 ctgttgcagc tcgtaataaa gctgaggagt tagcaagcgc agcattatat agcagacaag
    14341 aattattatc acaggctttg tctggtaaaa cagtagattt aaagattgtt tcgacttctc
    14401 ttctgacgcc gaccagttta actgggggag aggagagtat gttaaaagat caggtaaagg
    14461 cattaaaaga gttaaactct aaaagggggg aaccaacgaa attattaatt cggaatagtg
    14521 atggtcttct gcaagaagta aaggttaatc taaaagtggt gacatttaac tttggtgtaa
    14581 atgaattagc gctaaaaatg ggcttaggct ggaggaatgt tgacaagctt aatggtgaat
    14641 caatatgttc tttgttgggg gataatttcc tcaaaaatgg tgtgattgga ggttgggctg
    14701 cagaagctat agaaaaaaat ccaccatgta agaatgatgt gatatatctg gctaaccaga
    14761 taaaagagat tctaaacaag aagttacaaa aaaatgataa tggagagccg tataaattgt
    14821 cacaaagaat ggctctattg gcttatacta taggtgctgt accttgctgg aattgtaaga
    14881 gtgggaagga cagaacaggt atgcaggatg ctgaaattaa aagagaaata ataagaaaac
    14941 atgaaacagg gcagttttct caattaaata gtaaattatc ctcagaagag aaaagattat
    15001 tttctactat tctaatgaat agtggtaata tggaaatcca agagatgaat actggtgtgc
    15061 ctggaaataa agttatgaaa aaattaccgc tatcttcgtt agagctgtct tatgctgaaa
    15121 gaatagggga ccccaaaata tggaatatgg tgaaagggta ttcgtcattt gtataagagg
    15181 aatatatgga agatttagca gatgttattt gccgagcctt gggtatccct tcaattgata
    15241 ccgatgatca agcaatcatg cttgatgatg atgtgttaat atatattgaa aaagaaggag
    15301 attcaataaa tttattatgt cctttttgtg cattgcctga aaatataaat gatcttatat
    15361 atgcgttgag cctaaattac tcagaaaaga tatgtttagc tactgatgat gaaggtggaa
    15421 gtctaatcgc gcgattagat cttaccggga taaatgaatt cgaggatata tatgttaata
    15481 cagaatatta tatttcgcgt gtgcgttggc tgaaggatga atttgcgcga agaatgaagg
    15541 ggtattaatg tcccgtttcg tatttatttt tctgtgtttt atcccatatt tagggagagc
    15601 tgattgttgg gataaggctg gtgaaaggta caacattcca tcgagtttgt taaaagcgat
    15661 tgcggaaaaa gaatccggat ttaataaatt tgcagtcaat gttaataaca atggaagtaa
    15721 agattatggt ataatgcaaa taaatgactt tcattctaaa agacttagag aaatgggata
    15781 tactgaggaa atgctaatta gtcatccatg tctttctgta cattatgcag caaagttatt
    15841 aaatgagttt atgctgatgt atggaagagg gtgggaggct gttggtgcct ataatgcagg
    15901 aacatctccg aaaaagaaaa aagaaagatt aaagtacgca gaggatatct ataaacgcta
    15961 tttgcggata gcagcagaga gcaagcagaa taatcgaagg atataagagg atttatgtct
    16021 gaggcaaaga actcaaatct tgcaccattc aggttattag tgaaactcac aaacggtgtc
    16081 ggggatgagt ttccgttata ttatgggaat aacttaattg ttctcggacg gacgatagaa
    16141 acacttgagt ttggaaacga taatttccca gaaaatatta ttccagtaac agatagtaaa
    16201 tcggatggta taatttattt ggccataagt aaagataata tctgtcaatt tagtgatgaa
    16261 aaaggtgaac aaattgatat caattcacaa tttaactcat ttgaatatga cggagtttct
    16321 tttcaactta aaaatatgat ggaggataaa tcgagagttc atatactaaa cggaatgtat
    16381 aataaaccct cggtgttttt tttctttgct gttattgttg tgttaattat aattttttcc
    16441 ctctctttaa agaaagatga agtaaaagaa attgctgaaa ttatagatga taagagatat
    16501 ggaatagtca acacaggaca gtgcaattat attcttgccg aaacacaaaa tgatgcggta
    16561 tgggcatctg tagccttaaa taaaagcgga tttaccaaat gcagatatat tctggtgtcc
    16621 aacaaagaaa taaatagaat tcaacaatat atcaatcaac gattcccata tatcaatttg
    16681 tatgtgttga atttagtgag tgataaagcg gaattactgg tatttttaag taaagaaaga
    16741 aatagttcaa aagataccga acttgagaaa ttgaaaaatg ctctgatagt tgaatttccg
    16801 tatatcaaaa atattaaatt caactatctt agcgatcata atgcaaggga ggatgctaaa
    16861 ggtattttca caaaggtaaa tgtccagtat aaagagattt gtgaaactaa taaagttaca
    16921 tattctgtca gagaagaact gacagatgaa aagcttgagc tcattaatag actgatttct
    16981 gatcataaaa atgtatatgg agatcagtat attgagtttt ctgtactttt gatagatgat
    17041 gattttaaag gtaaatcata tcttaacagc aaagacagtt atgtaatgtt gaatgataaa
    17101 cactggtttt ttttagataa aaataagtga ggataaaatg agtgttacag taccggataa
    17161 agattggact ctgagttcat tatctgaaac ttttgatgat ggaactcaaa cattacaagg
    17221 gcaactaaca tcggcactaa atgcattagc tgaaaatcct tcgaatccac agttgctggc
    17281 tgaataccaa agtaaattat ctgaatatac attatatagg aacgcgcaat ctaatactgt
    17341 gaaagtgatt aaggatgttg atgctgcaat tattcaaaac ttcagataat agggagcatt
    17401 catgaattac atttatccag tcaatcaggt tgatattatc aaagccagtg attttcaatc
    17461 tcaagagata tcaagcctgg aagacgtcgt gtcggctaaa tatagtgata ttaagatgga
    17521 tacagatatt caagtatcac aaataatgga gatggtaagc catccagaat cattaaaccc
    17581 agaatctttg gccaagttac agacgacgct ctcaaattat tcaataggag tatcattagc
    17641 tggcacgtta gcaagaaaaa cagtttcggc tgttgaaact ttattaaagt cttaatttat
    17701 atgattaggt ataaatgttt tattttattc ttgttgctga tgttgattgg atgtgagcaa
    17761 cgtgaagagt taatttctaa tttatctcaa agacaggcaa atgaaataat atctgtgcta
    17821 gaacgccata atattactgc tagaaaagtt gatggaggta aacaggggat ctcggtacaa
    17881 gtcgaaaagg ggacatttgc atcggcagtt gatttgatgc gcatgtacga tttgccaaat
    17941 ccggagagag ttgatatctc acaaatgttt cctacagatt cattagtgtc ttctccaaga
    18001 gctgaaaagg cccgtttata tagtgctatt gagcaacggc tggaacagtc tttagtttct
    18061 attggtggtg ttatttcggc aaaaatacat gttagctatg atcttgaaga aaaaaatata
    18121 tcttcaaaac cgatgcatat atcagtaatc gctatatatg actcaccgaa agagtctgaa
    18181 ctattagtta gtaatattaa gcgatttttg aaaaacacct tttctgatgt taagtatgaa
    18241 aatatatctg tcatattaac tccgaaagaa gaatatgttt atacaaatgt acaacctgtt
    18301 aaggaaatta aatcggaatt tttaacaaat gaagtaatat atgtatttct cgggatggct
    18361 gtactagttg tcattctttt ggtatgggca ttcaaaacag ggtggttcaa gagaaacaaa
    18421 atatgataag aatggatgga atttataaaa aatatctttc aataattttt gatccagcgt
    18481 tctatataaa tagaaatcgg ttaaatttgc cttctgaact gttagaaaat ggcgtaatca
    18541 gaagtgagat taataatctc ataattaaca aatatgattt aaattgcgat attgaacctt
    18601 taagcggggt aaccgctatg tttgttgcca actggaattt acttccagct gttgcttatt
    18661 ttatcggtag tcaagagtct cggttaatta atcatagtga gatggttatt agttattatg
    18721 ggggcaaaat cagtaagcaa ggggaggccg ctattcgaag tggtttttgg catcttattg
    18781 agtggaagga aaatatatct gttggtatat atgaaagaat aaacttatta tttaatccaa
    18841 ttgctttgga gggaaattat acgcctgtag aaagaaattt gtcccgatta aatgaaggta
    18901 tgcaatatgc aaaaaggcac tttaccggta tccagacatc atgcctatga tggtgttgtt
    18961 ataaagagaa ttgaaaagga attatgtaaa accataaaag atagagacac tgagagtaag
    19021 aagaaagcaa tttgtgttat aaaggatgct acgaaaaaag ctgaatcact gcgtatagat
    19081 gctgtatgcg atggttatca aattggtata cagactgctt ttgagcatat tattgactat
    19141 atttgtgaat ggaagttaaa gcaaaatgaa aacagaagaa atattgagga ctatataact
    19201 agtttgcttt cagaaaattt acatgatgag aggatcatta gcactttatt ggagcagtgg
    19261 cttagttcgt tgcgtaatac agtaacagaa ttaaaagtgg ttttgcccaa gtgtaattta
    19321 gcactgagga aaaagttaga acttgatctc cataaatatc gctcagatgt caagattatt
    19381 ttaaaatatt ctgaagggaa taattatata ttctgtagtg gtaatcaagt agtggagttt
    19441 agtcctcagg atgtaatatc aggtgttaaa atagaacttg cggaaaaatt aacaaaaaat
    19501 gataaaaaat atttcaaaga gttagcacat aagaaactga gacaaattgc agaagacttg
    19561 ttaaaagaga atcctgttaa tgattaatca aataaatgca agcaatgctc tgcagcaaag
    19621 attaaatagt gaagaatttg ttaatttaaa tgagagatta tcatcttcgc aatcatttga
    19681 tgagaatata atctatgaaa taatgcaata tttctcacag agtgaattaa attccataga
    19741 taatgatgaa ctgcacaata agattgaaca attattcaat tcacgttttc cttatcttac
    19801 agctgcacaa aaaagttcac ttcttaataa actcatagat gcaaatcaat atgtcgattt
    19861 gcatgaggga ttttatgcat cattgtccat atataacaat attgatttct atattaagac
    19921 tacaaccttc gattcattaa ttagtgtctt tgaagcaggg agagaggcag atgattcgac
    19981 atggtagtaa taagttgaaa atatttattt taagtatatt gctattaaca ctgagtgggt
    20041 gtgctttaaa gtcatcatct aattctgaaa aagaatggca tattgttcct gtaagtaagg
    20101 attatttttc tattccaaat gatttattat ggtcgtttaa tacaaccaat aaaagtataa
    20161 atgtttactc taaatgtatt agtggtaagg cggtttatag ttttaatgca ggtaaattca
    20221 tgggcaactt taatgttaag gaagtagatg ggtgcttcat ggatgcacaa aagatagcta
    20281 tagataaact attttctatg ctgaaagacg gggttgtttt aaaaggtaat aagataaatg
    20341 ataccatcct tatagagaag gatggggaag ttaaattaaa attaattcga gggatataat
    20401 tgtattgtga gtaaatataa aggtctaaat acaagtaata tgttttacat ttactctagt
    20461 ggacatgaac cagttaacgt tgagcttgta aaagataaag aacgtaacat aattgagctg
    20521 gctccagcat ggaagggttt ttttttgtcc gaaatcaaaa tataaaattc agtgataatg
    20581 tgaactatca ttatcgcttt aacattaata gttgtgcaaa atttcttgct ttttgggact
    20641 atttcagtgg tgctctagta gagcatagcc atgctgaaaa atgtattcat ttctatcatg
    20701 agaatgatct tcgggatagt tgtaataccg aatcgatgct tgataagtta atgttaagat
    20761 tcatcttttc atctgaccag aatgtaagta atgcactggc tatgatacga atgactgagt
    20821 catatcattt ggttctttat cttctgagaa ctattgagaa agaaaaggag gtaagaataa
    20881 agagtctgac agagcattat ggtgtgtcgg aggcttattt tagatcttta tgtagaaaag
    20941 ctttaggggc aaaagtcaaa gaacaactga acacttggcg gttagtaaat ggtttattag
    21001 atgtattttt gcacaatcaa acaatcactt ctgctgccat gaataatggg tatgcatcaa
    21061 catcccattt ttcaaatgag ataaaaacac gactaggctt ttctgcaaga gaattatcga
    21121 atattacttt tttagtgaaa aaaataaatg aaaaaattta atattaaatc tttgactctc
    21181 ttgattgtat tgttaccatt gatagttaat gctaataata ttgactctca tttattagaa
    21241 caaaatgaca tagcgaaata tgttgctcaa agtgatacgg taggatcttt ttttgaacgt
    21301 ttttctgcgt tattaaatta tcctattgtt gttagcaaac aagctgcaaa aaagagaata
    21361 tctggggagt ttgatttatc taatccagaa gaaatgcttg aaaaaatgac attacttgtg
    21421 ggactcatat ggtataaaga tggaaatgca ttatacatat atgactctgg agaattaata
    21481 agcaaagtta ttttgctgga gaatatatca cttaattatc ttattcagta tctgaaggat
    21541 gctaaccttt atgatcacag atatcctata agaggaaaca ttagtgataa aacattctat
    21601 atatctggtc ctccggcatt ggttgaactg gtagctaata cagcaacttt gcttgataag
    21661 caggtttctt ctattgggac agataaagtt aattttgggg ttataaaatt aaaaaataca
    21721 tttgttagtg acagaactta taatatgaga ggggaggata ttgtcatccc aggggtggct
    21781 actgttgtag aacgtttatt aaataatggt aaggctctga gtaacagaca agccaaaaat
    21841 gatccgatgc caccctttaa tattacacag aaagtatctg aggatagtaa tgacttttca
    21901 tttagttcag ttaccaatag ttctatatta gaggatgtat cacttattgc atatccagaa
    21961 accaattcta tattagtcaa aggtaatgat cagcaaatac agattattcg cgatattatc
    22021 actcagttag atgtagccaa acgacatata gaattatcat tatggataat cgatattgat
    22081 aaatctgaat taaataacct tggcgttaat tggcaaggaa cggcttcatt tggtgattcg
    22141 tttggtgcat catttaatat gagctcatca gcatcgataa gcactttgga cggcaataag
    22201 tttattgctt cagttatggc attaaatcag aaaaagaaag caaatgtagt ttcaaggccg
    22261 gtgattttga cacaagagaa tattccggct atatttgata ataacaggac tttttatgtg
    22321 tcattagttg gtgagcgcaa ttcttcgttg gagcatgtta cgtatggaac attgataaat
    22381 gttatacctc gcttttcatc tcagggacaa attgagatgt ctttaacgat agaggatgga
    22441 acaggaaata gtcagtcaaa ttacaattat aataatgaaa atacatctgt ccttccagaa
    22501 gttggaagga ccaaaatcag tacaatagcg agagttccac aaggcaaaag tttgttgata
    22561 ggtggttata cgcatgaaac gaactctaac gaaatagtat ctattccttt tctctcctct
    22621 attcctgtaa tcggtaatgt gttcaagtat aaaacaagga atatcagtaa tattgtaagg
    22681 gtttttctga ttcaaccccg tgagataaag gagtcaagtt attataatac tgcagaatat
    22741 aagagcctca tatctgaaag agaaatacaa aagactacac aaataatccc atctgaaact
    22801 actttgctgg aagacgaaaa atcattggtt tcatacttaa attactaact ataaagtagg
    22861 tgatgtatgc ttgatgttaa aaatacagga gtttttagct ctgcattcat tgataagttg
    22921 aatgcaatga caaattcaga tgatggagat gagactgctg atgcagagct tgattctggc
    22981 ttggctaata gcaagtatat tgactcatct gatgagatgg cttccgctct ttcgtcattt
    23041 ataaacagaa gagaccttga gaaactgaaa ggaacaaata gtgatagtca ggaacgtatt
    23101 ttagatgggg aagaagatga aattaatcac aagatttttg atttaaagag aactttaaaa
    23161 gataaccttc ctctagatca ggattttata gacagactaa agagatattt taaagatcca
    23221 agtgatcaag tcttagcatt aagggaactt ttgaatgaaa aagatcttac tgctgaacaa
    23281 gtcgaatcat taactaaaat tattaatgag ataatatcag gtagtgaaaa aagtgttaat
    23341 gctggaataa attcagctat acaggctaaa ttatttggca acaaaatgaa acttgaacca
    23401 cagcttttgc gtgcatgtta tcgtggtttt atcatgggga acatatcaac aacagatcag
    23461 tatatagaat ggcttggtaa ttttggtttt aatcacagac atacaattgt gaattttgta
    23521 gagcagtcac tgattgtaga catggattct gagaaaccga gctgtaatgc ttatgagttt
    23581 ggttttgtgt tatctaaatt aattgcaatt aagatgatta gaacttcaga tgtaattttt
    23641 atgaagaaac tggaatcctc aagcttgcta aaagatggca gtttaagtgc agagcagcta
    23701 ttgctaactt tattatatat ttttcaatat ccaagtgaaa gtgagcaaat tcttacttct
    23761 gttatagacg tatcacgagc cagtcatgag gattctgtag tgtatcaaac atatctatct
    23821 tctgttaatg aaagtcctca tgatatattt aaaagtgaaa gtgaaagaga aattgcgatc
    23881 aatattctac gagagcttgt cacaagtgca tacaagaaag agctttctag ataacaggag
    23941 atgaaagtga tccagtcttt tcttaagcaa gtaagtacta agcctgaatt aattatatta
    24001 gtgcttatgg ttatgatcat tgctatgttg atcataccat tacctacata ccttgttgat
    24061 tttttaatag ggcttaatat tgtacttgct attctcgttt ttatgggatc attttatatt
    24121 gaaagaatat tgagtttttc tacattccct tcggttttgt tgataacgac attatttcgt
    24181 ctcgctttat caataagtac tagtcgacta attttagttg atgcggatgc gggtaaaatt
    24241 attactacat ttggccagtt tgtcattggt gatagtctgg ctgtaggttt tgttattttt
    24301 tcaatagtaa ctgttgtgca gttcattgtt ataacaaaag gatctgaaag ggttgcggag
    24361 gtagctgctc gcttttctct tgacggtatg ccggggaaac aaatgagcat tgatgcggat
    24421 ttaaaggctg gaattattga tgccgcagga gctaaagaaa gacgcagtat tctggaacgt
    24481 gagagtcaat tgtatggttc atttgatgga gcaatgaagt tcattaaagg tgatgcaatt
    24541 gctggcatca ttattatttt tgtcaattta ataggaggta tttctgttgg tatgagtcaa
    24601 catggaatgt ctctatctgg tgcattatct acttatacca tactaactat tggtgacgga
    24661 ttggtctctc agattcctgc tctgttaatt tctataagtg caggatttat cgttacaaga
    24721 gttaatggtg atagcgataa tatgggacgt aacataatgt cccagatatt tgggaatcct
    24781 tttgttctca ttgttacatc agctcttgcc ttggcgatag ggatgttgcc aggttttccg
    24841 ttttttgttt ttttcctgat agcagttact ttgacggctt tattctatta taaaaaggtc
    24901 gtagaaaaag aaaaaagtct gtctgagtct gattctagtg gctatactgg tacatttgat
    24961 attgataata gccatgactc atctttggca atgatagaaa atctggatgc tattagttca
    25021 gaaaccgttc ctttaatttt attatttgcc gaaaataaga taagtgcaaa tgatatggaa
    25081 ggtcttattg aaaggataag aagtcagttc tttattgatt atggtgtcag gcttccaact
    25141 attttatata gaacaagcaa tgagcttaag gtcgatgata ttgttttgtt aataaatgag
    25201 gtgcgtgccg atagttttaa tatatacttt gacaaagtgt gtattacaga tgaaaatgga
    25261 gatatagatg ctctaggtat tcctgttgtt tcaacttcat ataatgaacg tgttatttct
    25321 tgggtagacg tttcatatac agaaaatcta actaatattg atgctaaaat taaaagtgct
    25381 caagatgaat tttatcacca gttgtcacaa gctttattaa acaacataaa tgagattttt
    25441 ggtatacaag aaacaaaaaa tatgttagat cagtttgaaa atcggtatcc tgatctatta
    25501 aaggaagtct tccgacatgt gactatacag agaatttctg aggtattaca aagattgctt
    25561 ggagaaaata tttctgttcg caatttaaaa cttattatgg agtctttggc gctttgggct
    25621 cccagagaaa aagatgtcat aacattagtt gaacatgtcc gtgcatcact ttctaggtat
    25681 atttgtagta aaatagctgt ttctggtgag attaaagttg tgatgctttc cggatatatt
    25741 gaggatgcaa taagaaaagg gataaggcaa acctctggtg gctctttctt gaatatggat
    25801 atagaggttt cggatgaggt aatggaaact ttagcacatg ctttgagaga attgagaaat
    25861 gcaaaaaaaa atttcgttct tttggtatca gtagatatac gtaggtttgt taaaagactt
    25921 atagataaca gatttaagag tatactcgtt atatcgtatg ctgagattga tgaagcatat
    25981 accattaatg tattaaagac tatttagtga ggtttaaata tgagtaacat taatttagtt
    26041 caattagtta gagatagtct tttcacgatt ggttgtcctc catcaattat tacagattta
    26101 gacagccatt cagcgattac catttctttg gatagtatgc ctgctattaa tatagctctt
    26161 gtcaatgagc aggttatgtt atgggctaat ttcgatgcac caagtgatgt aaaacttcag
    26221 tcatctgctt ataacatatt aaatttaatg ttaatgaact ttagttatag cattaatgag
    26281 ctggtagagc ttcatcgttc tgatgagtac ttacagttaa gagttgttat taaagatgac
    26341 tacgttcacg atgggattgt ttttgcagag attctgcatg agttttatca gaggatggaa
    26401 atcttaaatg aggtcttata atcaatgagc tatataaaat tgctcactca attatctttt
    26461 cctaatagaa tctcggggcc aatcttggaa acaagtctta gcgatgtttc gattggtgag
    26521 atttgtaaca ttcaggctgg aattgaaagt aatgaaatta ttgcaagagc tcaggttgta
    26581 ggatttcatg atgaaaaaac aatattaagc ttgattggaa attctcgtgg actttcaagg
    26641 caaacgttga ttaagcccac tgcccagttt cttcatacgc aagtcggccg tggattattg
    26701 ggagcagtag tcaatccttt aggggaggtt actgataagt ttgctgttac ggataacagt
    26761 gaaattcttt atcgacctgt agataatgct cctccgctat atagtgaaag ggctgcaatt
    26821 gagaagcctt ttttaacagg tgttaaggtt attgattctt tactcacgtg tggtgaagga
    26881 cagcgaatgg ggatttttgc gtcagctggt tgtggcaaaa cttttctcat gaatatgctc
    26941 attgaacata gtggtgctga tatatatgtt attgggttaa ttggtgagcg aggtcgagag
    27001 gttacagaaa cggttgatta tttgaagaac tctgagaaaa aaagcaggtg tgttttagta
    27061 tatgcaactt cggattactc ttcggttgat cgttgtaatg ctgcatatat agccactgct
    27121 atagccgaat tttttaggac tgaaggacat aaagtagcgc tttttattga ttcattaaca
    27181 aggtatgcca gagcattacg tgatgtggcc ttagccgctg gagaatcacc tgccagaaga
    27241 ggctatccgg tttcggtttt tgatagctta cccagacttc ttgaaaggcc aggaaagtta
    27301 aaggcaggtg gctctattac tgcattttac actgttcttt tggaggatga tgattttgct
    27361 gatccattag ctgaagaggt aagatccatt ttagatggac atatatattt gagcagaaat
    27421 ctagcccaaa aaggacaatt tcctgcaatt gattccttaa aaagtataag cagggtattt
    27481 acacaggttg ttgatgaaaa acatcgtatt atggccactg catttcggga gctactttct
    27541 gaaatagaag agctaaggac aattattgac tttggtgaat acaaaccggg ggagaatgcc
    27601 tctcaggata aaatctacaa caaaatatct gttgttgaga gttttctgaa gcaagattat
    27661 cgactcggtt ttacttatga gcagacaatg gagcttcttg gtgaaacaat tagataaggt
    27721 tttaaaaatc aaagataaat atcaacggtc tgttaaatta attgaagcac acatattaac
    27781 actaaaaaaa aaactctttg tatcgagatg tggaggcatt agataaaagg attatatact
    27841 tcctgcaact ggagaatgac ttggaacctg taggcgctca gagtgttagt cagcttttca
    27901 atacaagacg taagatcgct atagtaaaaa agcatattat ccaatatcag tctgaacgaa
    27961 ttcttctaaa gggaagaatt gaagaaattc aaaaagatat tgatgaggca aatgcttcta
    28021 aaagaaaact gcttcataaa gagagtaaaa tatgtaaacg gattggcttg attaaaagaa
    28081 ataactttgc caaacagttg atacttgatg agttatcaca agaggatatg aagtatggca
    28141 ttagataata taaacctaaa ttttagtagt gacaaacaga tagaaaaatg tgagaaacta
    28201 tcttcaatag ataatattga tagtctcgtt ttgaagaaga agaggaaggt tgaaattcct
    28261 gagtactctt taattgcatc taactatttt actattgata agcactttga acataagcat
    28321 gataaaggag aaatttatag tggcattaaa aatgcgttcg aacttagaaa cgaacgagcg
    28381 acatattctg atattccaga atcaatggcc attaaagaaa atattttgat accagatcaa
    28441 gatatcaaag caagggaaaa aataaatatc ggcgatatga gggggatctt ttcatataat
    28501 aagagtggaa atgcagacaa gaacttcgaa agaagtcata cttcttctgt aaaccctgat
    28561 aatctgctag aatctgataa tagaaatggt caaattggtt taaaaaatca tagcttgtct
    28621 attgataaga atattgctga catcatttct ttactaaatg gaagtgttgc taaatcattt
    28681 gagctgcctg taatgaataa aaatactgca gacataaccc catccatgtc attgcaagaa
    28741 aaatcaatag ttgaaaatga taaaaatgtt tttcaaaaaa atagtgaaat gacttaccac
    28801 tttaaacagt ggggggctgg acattctgtt agtatttcag tggagtctgg ttcttttgtt
    28861 ctaaaaccgt cagatcaatt tgtaggaaat aaacttgact taattttgaa acaagatgct
    28921 gagggtaatt acagatttga tagcagtcaa cataataagg ggaataaaaa taatagtaca
    28981 ggatataatg aacagagtga agaagaatgc taagaattaa acattttgac gctaacgaaa
    29041 aactacagat tttatatgca aagcaactct gcgagcgttt ttcgattcag acattcaaaa
    29101 ataaatttac aggcagtgaa agtttagtca ctcttacttc cgtgtgtggg gattgggtaa
    29161 ttcgtattga tacattatct tttttgaaaa aaaaatacga ggtattttca ggattttcta
    29221 cacaagaatc tttactgcat ttatcaaaat gtgtctttat agagtcgtca tctgtatttt
    29281 cgattccaga actgtctgat aagattactt tccggatcac gaatgaaatc cagtatgcaa
    29341 ctactgggag tcatttatgc tgtttttcct cttctttagg tattatttat tttgacaaga
    29401 tgccggtatt acgtaatcaa gtttctcttg acttattgca tcatctttta gagttttgct
    29461 taggttcatc taatgtaagg ctggctactt taaaaagaat tcgtactggt gatataatca
    29521 tagttcagaa actttataat ttattattgt gtaatcaagt tattattggg gattatattg
    29581 tgaatgataa taatgaggca aaaattaatc tgtcagaaag taatggtgag tcagatcaca
    29641 cagaagtttc tttggcatta ttcaattatg atgatatcaa tgtaaaagtg gactttatcc
    29701 ttttagaaaa aaagatgaca atcaatgaac taaaaatgta tgtagaaaac gaattattca
    29761 agtttcccga tgacatagtt aaacatgtaa atattaaagt aaatggttct ttggttgggc
    29821 atggggaact tgtttctatt gaggatggtt atggtatcga gattagttct tggatggtaa
    29881 aggagtaatc tcatgctgag tgatatgtcc ctcatcgcta cactttcttt ttttacgcta
    29941 ttaccatttt tagttgctgc tggtacttgt tatataaagt tttctattgt ttttgtaatg
    30001 gtacgaaatg cacttggttt gcagcaggtt ccatcaaata tgactctcaa tggtattgca
    30061 ttgattatgg ctttgttcgt gatgaaaccc attattgaag ctggttatga aaattatcta
    30121 aatggcccgc aaaaatttga tacaatatca gatattgtcc gtttttctga ttctggcttg
    30181 attgaataca agcaatactt gaaaaagcat acagatcttg agttggcacg ttttttccaa
    30241 aggtctgaag aagaaaatgc tgatttaaag agtgcagaga ataatgatta ttcattgttt
    30301 tccctcttgc ctgcatatgc gctaagtgaa attaaagatg cgttcaaaat aggtttttat
    30361 ttatatttgc catttattgt tgttgatctt gttatttcaa gcattttact tgcacttggt
    30421 atgatgatga tgagtccaat cacaatatca gtaccaataa aacttgtatt atttgttgcg
    30481 cttgatgggt gggggatcct gtctaaggcc ttgattgaac aatatatcaa tgttcctgct
    30541 tagatgatgg aggtaatgtg tctgatatag tttatatggg taataaggct ctttatttaa
    30601 tccttatctt ttccttatgg ccagtaggta tagctacggt tattggatta actattggtt
    30661 tattacagac agtgactcaa cttcaagagc agacacttcc ttttggtata aagcttatag
    30721 gtgtctcaat atctttgcta cttctttctg gatggtatgg tgaggtttta ttgtcttttt
    30781 gtcatgaaat aatgttttta attaagagtg gggtttgatg gacatttcaa gctggttcga
    30841 aagtattcat gtgtttttaa tactcctgaa cggcgttttt tttagattgg ctccattatt
    30901 tttctttctt ccatttttaa ataacggtat aatttctcca tctattagaa tacctgtgat
    30961 ttttcttgtt gcatcaggat taattacttc tggtaaggta gacataggtt cttctgtttt
    31021 tgaacatgtt tatttcctta tgttcaagga aataattgtt ggcctccttc tctctttttg
    31081 cttgtctctt cccttttgga tatttcatgc tgttggtagt attattgaca accagcgtgg
    31141 ggcaacgctt agtagttcaa ttgatcctgc caatggtgtt gatacgtctg aattggcaaa
    31201 atttttcaat cttttttctg cagttgtatt tctatacagt ggtggaatgg tctttatttt
    31261 agaatccata caattgtctt ataatatatg cccgttattt tctcaatgtt ctttccgtgt
    31321 ctcaaatatc ttaacatttc tgactttatt ggcaagtcag gctgttattt tagccagtcc
    31381 tgttatgata gtattgttac tatcagaagt attacttggt gtattatcga gatttgctcc
    31441 gcagatgaat gctttttccg tatcattaac tattaaaagt ttacttgcaa tatttattat
    31501 tttcatctgt tcttctacta tttacttttc taaagttcaa tttttcctcg gtgaacataa
    31561 atttttcaca aatctatttg ttagataaaa tattatggca aataaaacag aaaagccgac
    31621 acctaaaaaa ctaaaggatg ccgcaaaaaa aggacagtca tttaaattta aggatttaac
    31681 gacagttgtt attattctgg tagggacatt tactataata tcattctttt ccttaagtga
    31741 tgtaatgctc ttatacagat atgtaataat taatgacttc gaaattaatg agggtaaata
    31801 cttttttgca gtggttattg tcttttttaa gataattggc ttcccacttt ttttctgtgt
    31861 tctttcggct gtgttgccaa cattggttca aacaaagttt gttcttgcga ctaaagctat
    31921 caagattgat ttttcagtat taaaccctgt taaagggtta aaaaaaatat ttagtataaa
    31981 gacaataaaa gaatttttca aaagcattct gcttcttatt attctagcat taacaaccta
    32041 tttcttttgg attaatgacc gaaaaataat tttttctcag gtgttttcta gtgttgatgg
    32101 cttatatctt atttgggggg ggctatttaa ggatataata ttatttttct tagcattttc
    32161 tatttttgtt attattcttg actttgtgat tgagttcatt ttatacatga aagatatgat
    32221 gatggataaa caggagataa aaagagaata tatagagcaa gagggacact ttgagacaaa
    32281 gtcgagaagg cgtgagttgc atatcgagat tctttcagag cagactaaat ctgatatacg
    32341 taattcaaaa ttagtggtaa tgaacccgac tcatattgca attggtattt attttaatcc
    32401 agaaatagcg cctgcacctt ttatttctct cattgaaact aaccagtgtg ccttggctgt
    32461 cagaaaatat gcaaatgaag ttggtatacc aactgtgcgt gatgtgaaat tagctagaaa
    32521 actatataaa acacatacaa aatatagttt tgttgatttt gaacacttgg atgaagtcct
    32581 acgtcttatt gtttggcttg agcaggttga aaacactcat taataagtaa aggagatgtg
    32641 ttatatggga gttaatttct gtaataaaat aggtattgat cagtctgaat ttgaaataga
    32701 atcttccatc attaactcca ttgctaatga ggtattgaac ccaatatctt tccttagcaa
    32761 caaggatata ataaatgttt tactcagaaa gatttcatct gaatgtgacc tggttagaaa
    32821 agacatttat cgttgtgctc ttgagttggt cgttgaaaaa acacctgatg atttataatc
    32881 attcataata actcttttgt tgatataaat atttttgttg aagatattta tgtagtcaca
    32941 ggatcaattg tagaaaattg ttatgttttt gttaggaact gaactaaata atatatgata
    33001 agacagcaaa aacgactgac cataatcctt cttttattag gagttgataa gagagactat
    33061 tcatcctgta atgttaaaac tctgttatat agtattcgcg attatgcaaa gtctgtaaat
    33121 gatcatgaga tactaaccga gagtaatcgt ttattatctc attgcatttc agatagtaac
    33181 ggagctttct ttaaaagtag taaatacgtt ccccttaagt acttacgtaa aaggagaata
    33241 gcacgaaaga taccaaatga ttagcacttt gctagtaagc ttgctggtaa actatattga
    33301 ccgttgggtt tgttttgaga ctgtaattta aattgtgtaa ttgcctgttt ttgatatgtt
    33361 tactccaaca acggagacag gtaaattatg gacgaaaaga agcttaaagc ccttgcggct
    33421 gaactggcta aaggcctcaa aactgaggcc gacctcaatc agttttcccg tatgctgacg
    33481 aaatatcccg gctcttaaaa cgatagtggc tcaggctttc atttttcagc gttccccaga
    33541 agatttccat cggagcgttg tcgtaacagt tacctttacg cgacattgat gttttcagac
    33601 cagactgctc ctgtatgacc cggtaatcgt atgcgcagta ctgtgaacct cgatcggtgt
    33661 ggtggattag cccggcaggt gggcgctggc tcctgagcgc cataaacagg gctttacccg
    33721 tcaattcttt cgtcatgcgc tctcccatgg cgtaaccgac aatttcgcac gtataaacat
    33781 ctttgacgcc ggcgagatac agccatcctt cccgggtggc aacatacgtt atatctgcga
    33841 cccagacctg attaggtgct gtgggcgtaa agttctgatt cagcagattc ggcgtaaccg
    33901 gcggattgtg gtcagag
//
LOCUS       DAJTMC010000027        32956 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.027, whole genome shotgun sequence.
ACCESSION   DAJTMC010000027 DAJTMC010000000
VERSION     DAJTMC010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32956
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.027"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            <1..257
                     /locus_tag="OO130_001131"
     CDS             <1..257
                     /locus_tag="OO130_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HCR5364623.1"
                     /translation="LDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNGLSVV
                     MTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            267..1641
                     /gene="gspE"
                     /locus_tag="OO130_001132"
                     /pseudo
     CDS             267..1641
                     /gene="gspE"
                     /locus_tag="OO130_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414649.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
     gene            1631..2833
                     /gene="hofC"
                     /locus_tag="OO130_001133"
     CDS             1631..2833
                     /gene="hofC"
                     /locus_tag="OO130_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HCR5364624.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRALLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEVFPPLYQAMIRTGELTGKLDECCFELARQQKAQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWSW
                     LLVLFGFLLAIANKLLMRRPTWLIVRQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNTGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(2868..3911)
                     /gene="guaC"
                     /locus_tag="OO130_001134"
     CDS             complement(2868..3911)
                     /gene="guaC"
                     /locus_tag="OO130_001134"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HCR5364625.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSDQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            complement(4067..4111)
                     /locus_tag="OO130_001135"
     CDS             complement(4067..4111)
                     /locus_tag="OO130_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5364626.1"
                     /translation="MIIKTLKMSARKRN"
     gene            4136..4756
                     /gene="coaE"
                     /locus_tag="OO130_001136"
     CDS             4136..4756
                     /gene="coaE"
                     /locus_tag="OO130_001136"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HCR5364627.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            4756..5499
                     /gene="zapD"
                     /locus_tag="OO130_001137"
     CDS             4756..5499
                     /gene="zapD"
                     /locus_tag="OO130_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HCR5364628.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDSENGQVPERLDFELACC"
     gene            5509..5706
                     /gene="yacG"
                     /locus_tag="OO130_001138"
     CDS             5509..5706
                     /gene="yacG"
                     /locus_tag="OO130_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HCR5364629.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(5813..6202)
                     /gene="mutT"
                     /locus_tag="OO130_001139"
     CDS             complement(5813..6202)
                     /gene="mutT"
                     /locus_tag="OO130_001139"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HCR5364630.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLAYEFPDRHITLWFWLVESWEGVPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(6262..8967)
                     /gene="secA"
                     /locus_tag="OO130_001140"
     CDS             complement(6262..8967)
                     /gene="secA"
                     /locus_tag="OO130_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HCR5364631.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(9029..9541)
                     /gene="secM"
                     /locus_tag="OO130_001141"
     CDS             complement(9029..9541)
                     /gene="secM"
                     /locus_tag="OO130_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HCR5364632.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNVPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQYAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(9772..10689)
                     /gene="lpxC"
                     /locus_tag="OO130_001142"
     CDS             complement(9772..10689)
                     /gene="lpxC"
                     /locus_tag="OO130_001142"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HCR5364633.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFIAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(10790..11941)
                     /gene="ftsZ"
                     /locus_tag="OO130_001143"
     CDS             complement(10790..11941)
                     /gene="ftsZ"
                     /locus_tag="OO130_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HCR5364634.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(12002..13264)
                     /gene="ftsA"
                     /locus_tag="OO130_001144"
     CDS             complement(12002..13264)
                     /gene="ftsA"
                     /locus_tag="OO130_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HCR5364635.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(13261..14091)
                     /gene="ftsQ"
                     /locus_tag="OO130_001145"
     CDS             complement(13261..14091)
                     /gene="ftsQ"
                     /locus_tag="OO130_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HCR5364636.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKETAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQLAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(14093..15013)
                     /gene="ddlB"
                     /locus_tag="OO130_001146"
     CDS             complement(14093..15013)
                     /gene="ddlB"
                     /locus_tag="OO130_001146"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCR5364637.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLDASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(15006..16481)
                     /gene="murC"
                     /locus_tag="OO130_001147"
     CDS             complement(15006..16481)
                     /gene="murC"
                     /locus_tag="OO130_001147"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HCR5364638.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFLGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(16535..17602)
                     /gene="murG"
                     /locus_tag="OO130_001148"
     CDS             complement(16535..17602)
                     /gene="murG"
                     /locus_tag="OO130_001148"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HCR5364639.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(17599..18843)
                     /gene="ftsW"
                     /locus_tag="OO130_001149"
     CDS             complement(17599..18843)
                     /gene="ftsW"
                     /locus_tag="OO130_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HCR5364640.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVVFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(18843..20159)
                     /gene="murD"
                     /locus_tag="OO130_001150"
     CDS             complement(18843..20159)
                     /gene="murD"
                     /locus_tag="OO130_001150"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HCR5364641.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDAECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     QVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(20162..21244)
                     /gene="mraY"
                     /locus_tag="OO130_001151"
     CDS             complement(20162..21244)
                     /gene="mraY"
                     /locus_tag="OO130_001151"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HCR5364642.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(21238..22596)
                     /gene="murF"
                     /locus_tag="OO130_001152"
     CDS             complement(21238..22596)
                     /gene="murF"
                     /locus_tag="OO130_001152"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HCR5364643.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(22593..24080)
                     /gene="murE"
                     /locus_tag="OO130_001153"
     CDS             complement(22593..24080)
                     /gene="murE"
                     /locus_tag="OO130_001153"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HCR5364644.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVEQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKV
                     IDVNYHDSGVTIRFSSSWGDGEIESHLIGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTVPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(24067..25833)
                     /gene="ftsI"
                     /locus_tag="OO130_001154"
     CDS             complement(24067..25833)
                     /gene="ftsI"
                     /locus_tag="OO130_001154"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HCR5364645.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(25849..26214)
                     /gene="ftsL"
                     /locus_tag="OO130_001155"
     CDS             complement(25849..26214)
                     /gene="ftsL"
                     /locus_tag="OO130_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HCR5364646.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(26211..27152)
                     /gene="rsmH"
                     /locus_tag="OO130_001156"
     CDS             complement(26211..27152)
                     /gene="rsmH"
                     /locus_tag="OO130_001156"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HCR5364647.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(27154..27612)
                     /gene="mraZ"
                     /locus_tag="OO130_001157"
     CDS             complement(27154..27612)
                     /gene="mraZ"
                     /locus_tag="OO130_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HCR5364648.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
     gene            complement(28214..29218)
                     /gene="cra"
                     /locus_tag="OO130_001158"
     CDS             complement(28214..29218)
                     /gene="cra"
                     /locus_tag="OO130_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HCR5364649.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANAPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHLMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(29300..29386)
                     /locus_tag="OO130_001159"
     CDS             complement(29300..29386)
                     /locus_tag="OO130_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HCR5364650.1"
                     /translation="MCNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(29398..29889)
                     /gene="ilvN"
                     /locus_tag="OO130_001160"
     CDS             complement(29398..29889)
                     /gene="ilvN"
                     /locus_tag="OO130_001160"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCR5364651.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIREVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(29892..31616)
                     /gene="ilvI"
                     /locus_tag="OO130_001161"
     CDS             complement(29892..31616)
                     /gene="ilvI"
                     /locus_tag="OO130_001161"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HCR5364652.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTTGHKGQIKRALQTLVVAKKPVVYVGGGAI
                     MAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPISISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTKGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            31735..31779
                     /locus_tag="OO130_001162"
     CDS             31735..31779
                     /locus_tag="OO130_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5364653.1"
                     /translation="MKRNDKILHYEGLN"
     gene            complement(31934..32878)
                     /gene="leuO"
                     /locus_tag="OO130_001163"
     CDS             complement(31934..32878)
                     /gene="leuO"
                     /locus_tag="OO130_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HCR5364654.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
BASE COUNT         7911 a         9256 c         8018 g         7771 t
ORIGIN      
        1 gattagatac ctgcgacggt ggcagcaatg gcattccctc gcctaccacc acccgctatg
       61 tttcagccat gagtgtggca aaaggcgtgg tgtcgctgac cgggcaagaa agtctcaatg
      121 gactaagcgt cgtcatgacg ccaggttggg ataacgcaaa cggcgtcacc ggctgggcgc
      181 gcaactgcaa tattcaaagt gacagcgcat tgcagcaagc ctgcgaagat gtcttccgct
      241 ttgatgacgc caactaagga gcggcaatga atattccaca gctcaccgcc ctgtgtctgc
      301 gttatcaggg agtcttgctg gatgccagcg aagaggtggt tcatgttgcg gtagtcgatg
      361 caccttcgca tgagttgctg gacgcattgc atttcgctac caccaaacgt attgagatca
      421 cctgctggac gcgccaacaa atggaaggcc acgccagtcg cacacaacag acattgcccg
      481 tagctgttca ggagaagcat cagtcgaaag cagagttgct gactcgaacg ttacaatctg
      541 cgctggaaca acgtgcgtct gatattcata tcgaaccagc ggacaatgcc taccgcatcc
      601 gcttgcgtat cgacggcgta ttgcatcctt taccggatgt ttcaccggat gccggagtcg
      661 cattaaccgc cagattaaaa gtgctgggaa acctggatat tgcggaacat cgcctgccgc
      721 aggacgggca attcactgtc gaactggcag gaaacgccgt ctcatttcgt attgcgacct
      781 taccatgtcg gggtggtgaa aaggtggtat taaggttgtt acagcaggtg aaccaggcac
      841 tggatgttaa cacgctggga atgcagccgt tacaactggc ggactttgct catgccttgc
      901 aacaaccaca gggactggtg ctggtaactg gccctacagg cagcggcaaa acggtcacgc
      961 tttatagtgc cctgcaaaag ctgaataccg ctgacattaa tatttgtagc gtcgaagatc
     1021 cggttgagat ccccatagcc ggactaaacc agacgcaaat ccatccgcgt gccgggctca
     1081 cctttcaggg cgttttgcgt gcgttattgc gccaggatcc tgacgtcatc atgatccgat
     1141 ggcgaaacgg cagaaattgc cattaaagcc gcgcaaaccg gtcacctggt gttgtctacc
     1201 ctacacacta attccacctg cgaaacgctg gtacgtttac agcaaatggg agtcgcccgc
     1261 tggatgctct catcagcgct tacgctggta atagcccagc gtctggtacg taaattttgc
     1321 ccacattgtc gccagcagca aggggagccc atccatattc cagacaatgt atggccgtcg
     1381 ccgctgcccc actggcaggc acccggttgt gtacattgct accacggttt ttatggtcgt
     1441 accgccttgt ttgaagtcct gcccattaca ccagccatac gccagcttat ttccgctaat
     1501 accgacgttg aatcgctgga aacgcacgca cgacaggcgg gtatgcgtac gctttttgaa
     1561 aacggctgcc tagccgtgga gcaaggctta accacctttg aagagttaat ccgcgtactg
     1621 gggatgccgc atggcgagta agcaactctg gcgctggcat ggcataaccg gcgacggcaa
     1681 tgcgcaagat gggatgctat gggcagagag ccgtgctttg ctgctcatgg cactacagca
     1741 acagatggtt accccactta gcctgaagcg aatcgccatc aattctgcgc agtggcgagg
     1801 agataaaagc gcggaagtca ttcatcaact ggcgacgcta ctcaaagccg ggttaacgct
     1861 ttctgaaggg ctggcactgc tggcggaaca gcatcccagt aagcaatggc aagcgttgct
     1921 gcaatcgctg gcgcacgatc tcgaacaggg cattgctttt tccaatgcct tattaccctg
     1981 gtcagaggta tttccgccac tctatcaggc gatgatccgc acgggtgaac tgaccggtaa
     2041 gctggatgaa tgctgctttg aactggcgcg tcagcaaaaa gcccagcgtc agttgaccga
     2101 caaagtgaaa tcagcgttac gttatcccat catcatttta gcgatggcaa tcatggtggt
     2161 tgtggcaatg ctgcattttg ttctgccgga gtttgccgct atctataaga ccttcaacac
     2221 cccactaccg gcactaacgc aggggatcat gacgctggca gactttagtg gcgaatggag
     2281 ctggctgctg gtgttgttcg gctttctgct ggcgatagcc aataagttgc tgatgcgccg
     2341 accgacctgg cttatagtgc ggcagaaatt gctgttacgc atcccgatta tgggttcact
     2401 gatgcgggga caaaaactca cgcagatctt tacgattctg gcgctgacac aaagtgcagg
     2461 cattactttt ttgcagggcg tagagagcgt cagagaaaca atgcgctgcc cgtactgggt
     2521 gcaacttctg acacaaatcc agcacgatat cagtaacggt catcccatct ggctggcgct
     2581 aaaaaatacc ggggagttta gcccgctctg tttgcaatta gtgagaacag gagaggcatc
     2641 cggctcgctg gacctcatgt tagacaacct cgcccatcat catcgggata acacaatggc
     2701 gctggcggat aacctcgcag ccttactgga accggcgttg ctgatcataa cgggaggaat
     2761 tatcggtacg ctggtggtgg cgatgtatct gccaattttc catttaggcg atgcgatgag
     2821 tgggatggga taacgctggc gtgttgctcc acaccagcgt tggaagatta caggttgttg
     2881 aagatgcggt tttcttgttc ctgcacacgg ataaatgtgg tgcgcttggt cagttctttc
     2941 aggcgtgaag ccccaacgta tgtacaagct gaacgcaggc cgcccaaaat atcacgcgcg
     3001 gtattttcaa ccgggcctcg cagcggcagc ttaacggttt taccttctgc tgcgcgatat
     3061 tccgcaacgc cgccaacgtg acgtttcatc gcagactcgg agctcatgcc gtagaacagc
     3121 ataaatttct cgccgttctc ctcaacgatg cgaccgccgc tctcttcgtg gcccgccagc
     3181 atgccgccaa gcatgacgaa atcggcaccg ccgccaaagg cttttgcgac atcgcccggc
     3241 gtggtgcagc caccgtcgct gacgatcatt ccgcccagac cgtgcgccgc atcggcacat
     3301 tcgattaccg cagaaagctg cggataaccg acgcctgttt tgacgcgagt tgtacaaaca
     3361 gaacctgggc caatgccaac tttaacgata tcagcaccgg agaggataag ctcctcacac
     3421 atttcaccag tcactacgtt gccagcacaa atggttttgg tcggccacgc ttcacgcgct
     3481 ttcgcaacga actgcacgaa gtgttcggaa taaccattcg ccacatcaat acaaacaaag
     3541 tttaatgccg ggttcaggtc gagaatctgt ttagtttttt cgaaatccgc atcagacgta
     3601 ccggtagaaa ccatcacatg tttcagcaca tcagcggaag aattgttgat aaacgcttgc
     3661 cactcttcga cagaatagtg tttatgcaca gcagtcaaaa tatcaaaaga agccagcgca
     3721 gaggccatag aaaatgtacc tacggtgtcc atatttgcgg cgataatcgg cacgccggac
     3781 cagctctgat ctgaatgttt gaaggtgaat tgacgttcca gttcaacatc ggaacggctt
     3841 ttaagagtgg agcgtttagg gcggatgaga acgtctttaa aacctaactt cagatcttct
     3901 tcaatacgca tgtgcggatt cctggggtta atggctataa gggtaagagc gcaactccag
     3961 tgacgctatc atacgcacta atcaatgcgg cgcaagactg cgaaattgtc tttttttacg
     4021 ctacaatccc ataaatttac ctggtaaata gtgagcgaac gtttcatcaa tttcgctttc
     4081 gtgcactcat ttttaacgtt ttgataatca tgatgaattc ccgggaatat aaattatgag
     4141 gtatatagtt gccttaacgg gaggcattgg cagtggcaag agtaccgttg ccaatgcgtt
     4201 tgctgatctc ggaattaacg tcattgatgc cgatattatt gcgcgtcagg tggttgaacc
     4261 aggtgcacct gcgctacatg ccattgctga tcactttggc gctaacatga ttgctgctga
     4321 tggaacattg cagcgccggg ccttgcgcga gcggatcttc gccaacccgg aagagaaaaa
     4381 ctggcttaac gccctgctgc atccgctgat tcagcaagag acgcaacacc agatccagca
     4441 agctacttcc ccctatgtac tgtgggttgt gccattgctg gtagaaaact cactgtataa
     4501 aaaagcgaat cgagtgctgg tggtggatgt cagcccagaa acgcaactta agcgcaccat
     4561 gcagcgcgat gatgtaactc gcgagcatgt cgaacaaatc cttgctgctc aggcaacgcg
     4621 cgaagcccgc cttgccgtgg cagatgacgt cattgataat aacggcgcac cggatgctat
     4681 cgcatcggat gttgcccgcc tgcacgcaca ctatttgcag cttgcgtcgc agtttgtctc
     4741 acaggaaaaa ccgtaatgca gacccaggtc ctttttgaac atccactaaa tgaaaaaatg
     4801 cgtacatggc tgcgcattga gtttttgatt cagcaactca ccgttaattt acccatcgtt
     4861 gaccacgctg gcgcgctgca tttcttccgt aatgtcagtg aattactgga tgttttcgag
     4921 cgcggcgaag tccgcactga gctgttgaaa gaacttgacc ggcagcaacg taaactccag
     4981 acctggattg gcgtgcctgg cgtggaccag agccgtattg aagcattaat tcagcagtta
     5041 aaagcggcgg ggagcgtatt aatttccgcg ccgcgtatcg ggcaatttct gcgtgaagat
     5101 cgtttgattg ctctggtgcg tcagcgactg agcatcccag gcggctgttg cagctttgat
     5161 ttacctacat tgcacatttg gctgcatcta ccccaggcgc agcgcgacag ccaggtagaa
     5221 acctggattg ccagcctgaa cccgctcacc caggcactta ccatggtgct ggatttaatt
     5281 cgccagtcgg cccccttccg taaacaaacc agcctgaatg gtttttatca ggataacggt
     5341 ggcgatgccg acttgctgcg cctgaatctg tcgctcgatt cacagcttta tccgcaaatt
     5401 tccggacata agagccgttt tgccattcgt tttatgccgc tggacagtga aaacggacag
     5461 gtaccggaac gtctagattt cgaactggcc tgttgctaag gagtgaaaat gtcagaaact
     5521 attacggtga attgcccaac ctgcgggaaa acggtggtat ggggtgaaat cagcccgttt
     5581 cggccatttt gctccaaacg ttgtcagctg atcgacctcg gagaatgggc tgctgaagaa
     5641 aaacgaatcc ccagcagtgg cgatctttcc gaaagcgatg actggagcga agagccaaag
     5701 cagtgacatt ggtctgatgc cgcacatagg cctgataaga cgcggacagc gtcgcatcag
     5761 gcatcttgtg caaatgtcgg atgcggcgtg aacgccttat ccgacctaca gactacagac
     5821 gcttaagctt cgcaattacc ggttcattgg ctggcggaaa atcatcggca ttaagaccga
     5881 ccagcgacat ccactcaccg ggttgccctt ctttacccca cggcacccct tcccaacttt
     5941 cgaccagcca gaaccacaga gttatatgcc tgtccgggaa ttcatatgcc agtttttcaa
     6001 atagcgaaaa atgttgtggg gtaatcccga cctcttcctg aagttcacgc accaccgcct
     6061 gttccggcgt ttcgcccatt tcaattttac cgccgggaaa ctccagttta ttcgccatgt
     6121 gcgcatctgc tgcgcgacgc gttataaaga tttcattgtt ctcgttgcga ataataccta
     6181 ccgcaatttg cagctttttc attgttttaa acctataaaa aaggcgcaga atcctgcgcc
     6241 ttttgcttca acagttagct tttattgcag gcggccatga cactgcttgt attttttacc
     6301 agaaccgcac gggcaaggat cgttacgtcc tactttgcgc tcaccggttt gcgccgccag
     6361 tgcagctgcg gctgcagagt cgtcatcctg atggctaagc tgctgcattt gcgctaaacg
     6421 ctcggcttcc atacgacgct gttgttccag ctcctcaacc tcttcaggca tacgtacctg
     6481 aactttgctc agcgtactga taacttcata tttcaacgac tccagcatcg ctgcaaacat
     6541 ggagaacgat tcacgtttgt attcctgctt cggatctttc tgtgcatagc cacgcaggtg
     6601 gataccctga cgcagatagt ccatcgctgc caggtgctct ttccacaggg agtcaagcgt
     6661 ttgcagcatg acgcctttct cgaagtgacg catcatctca gcaccaacca cttcttcttt
     6721 acgctgatac acttcgatgg actgcgccag aatgcgctca cgcagcgtct cttcatgcag
     6781 ttctggttct ttatccagcc actcggcaat tggtaaatcg aggtcgaaat cgttcttcag
     6841 acgttcctgc agccccggaa tatcccacat ttcttccagc gactgtggcg gaatgtaggc
     6901 atcaatggtg gctttgaaca catcttcacg aatgctgttg atggtttcgc tcacatcgct
     6961 gacatccagc agttcgttac gctgggagta aatggcgcga cgctgatcgt tagccacgtc
     7021 atcatattcc agcagttgct tacgaatgtc gaagttacga ctttcaactt tacgctgggc
     7081 gttggcaatc gctttagtca cccacgggtg ttcaatggct tcgcctggct tcatacccag
     7141 tttacgcatc atgccggata ctcggtcgga agcaaaaata cgcatcagcg catcttccat
     7201 cgacaggtag aaacgggaag aaccagcatc cccctgacga ccagaacgac cgcgcaactg
     7261 gttatcgata cgacgggatt cgtgacgctc ggtaccgatg atatgcaggc cacctgcttc
     7321 cagtaccgca tcgtgacgta cctgccagtc ggctttaatt ttttcaattt gctctgcggt
     7381 cggattttcc agcgcggcaa cttctgcctg ccagctacca ccgagcacaa tatctgtacc
     7441 acgacccgcc atattggtcg cgatagtcac cgcagccgga taacctgctt gagcaacaat
     7501 cgccgcttcg ttggcgtgga atttggcgtt caggacgttg tgcttaatac cggctttggt
     7561 cagttcgttt gacaccagct ccgatttttc gatggagata gtacccacca gcaccggctg
     7621 gcctttcgca gtacgttctt tgatatcttc aatgatcgcc tgaatttttt ccgcttcagt
     7681 catgtagacc aggtccggca gatctttacg aatcattgga cggttggtcg gaacaacgac
     7741 ggtatccagc ttgtagatgg agctaaattc gaaagcttcg gtatcagcag taccggtcat
     7801 ccccgccagt ttttcataca gacggaagta gttctggaag gtgatcgaag ccagcgtttg
     7861 gttttcgttc tggatctgca caccttcttt cgcttccaca gcctggtgca gaccatcgga
     7921 ccagcgacgg ccctgcatgg tacgaccggt gtgttcgtca acgatgataa cttcaccatc
     7981 tttaacgatg tagtcgacgt cgcgggtaaa cagcgcatga gcgcgcagcg ccgccgttac
     8041 gtggtgcatc agcatgatgt tggccggaga gtacagagac tccccttcat ccatgatgcc
     8101 ctctttcacc agcagttctt caatcagcac cagaccacgt tcggtcaggt tcacctggcg
     8161 agatttttcg tccaccgaga agtggccttc gccctggaag gtttcggagt cttctttttc
     8221 ctgacggatc aggtgcggaa taattttatt cacgcgttta tacatttccg agctgtcttc
     8281 tgccgggccg gaaatgatca gcggtgtacg cgcttcatcg atcaggatgg agtccacttc
     8341 gtccaccagc gcatagtgca gtttacgctg tacacgttct tcagggctga acgccatatt
     8401 gtcgcgcagg tagtcaaagc cgtattcgtt gttcgtaccg taagtgatgt cagctgcgta
     8461 agcttcgcgc tttgccggtg ctggcatgcc cggcaggttg ataccgacag tcaggccaag
     8521 gaattcaaac agcggacggt tgttttcggc gtcacgttgc gccaggtagt cgttgacggt
     8581 aactacgtgc acgcctttac cggttagtgc gttcaggtaa gcaggcagcg ttgcggtcag
     8641 ggttttacct tcaccggtac gcatttcggc gatgcagcgt tcgttaagaa ccataccgcc
     8701 gagtaactga acgtcgaagt gacgcatacc aaagacgcgc ttacttgcct cacgtaccac
     8761 ggcgaaagct tccgggatca gattttccag cacttcgcct ttttccagac gtgcacgaaa
     8821 ctctgcggtt ttccctttca gttcttcgtc ggagagtttt tccatctccg gttccatggc
     8881 attgatgatg ttgaccactt tgcgcatccg gcgcagggtg cgatcgttac gactaccgaa
     8941 aactttagtt aacaatttga ttagcataat aaaatctcaa acgccccgcg ttgcggagtt
     9001 aataaaatga agtaaaggtt tattgttgtt aggtgaggcg ttgagggcca gcacggatgc
     9061 cttgcgcctg gcttatccag acgggcgtgc tgaattttgc ttgtggggta aaatgcgcat
     9121 aatcaatgcg ataacccttt tcagacggcg tgccttcctg ggtcagcagc gcgctgagcg
     9181 tatccagtaa tgcaagatgt tgcgcctgaa gaggcaaaga ttcttcagca acgggcagtg
     9241 tttgcggtgc cattgcgaaa gaaagatgac ggattaccgt gcgaatggca tattgatgcc
     9301 agtaatcaac ggaatagttc gaattcgggc ggcgtgtgtt cgcttccagc aaggccaatt
     9361 gaccaaagtt aactttggct gaaggctcgt ggttgcgggt tgtcgctttt gcgggcacgt
     9421 ttggttcggc ggcgttgctg agcgcaggca aacctaaact cgccgcgacc atccctaata
     9481 agagatgcgg ccagaagtag cgtttaccaa actgtcgcca gcgcgtcagt attccactca
     9541 cgttattgcc atcccgatcg aattcaaacg tatttagcgc acaaatctta tcattaaagc
     9601 ctgaagtcca cagcatgaat gaagataagt tgtgcgtaaa agtgcttaaa aaaacagcgt
     9661 tcgcaccgtt ttttatagct cttataaaat tcaaaaaaaa acgactggtt cacctggccg
     9721 gagagagtgc cagatttgcc agtcgaattt tatacgacag tataaatgtc gttatgccag
     9781 tacagctgaa ggcgctttga aggccaacgg cagttctgcg tcgtcctgga aggtcacata
     9841 ttcccaagct tcctgtttcg ccaggacagc ctgcagcagt ttgttattca gtgcatgacc
     9901 ggatttataa gcgataaatg caccaataat attgtgacca cacatgaaca agtcaccgat
     9961 cgcatcgagc attttgtgac gcacaaattc gtcttcaaaa cgcaggccgt cttcgttcag
    10021 tacgcgataa tcgtcaacaa cgatggcaca atcgaagctg ccgcccaggc acaaaccacg
    10081 ggactgcaga tattcgatat cacgcatgaa accgaacgta cgcgcacggc tgatctggcg
    10141 cataaacgca tcagcggaga agttcatcgc atagcgctgg ttgctggaat caatagccgg
    10201 atggttaaaa tcgatggtga aatccagcga aaaaccattg tacggcttaa attcagccca
    10261 cttatcgcca tcttcgacac gaacagtctc tttgatgcga acaaattttt tggcgcagtt
    10321 caactcgtcg ataccggcgt caagcagcag gtatacaaac ggagcggcgc tgccgtccat
    10381 gatcgggatt tccggcgcgt taacttcgat aacaatgtta tcgatgccca ggcccgcgag
    10441 agcagcattg aggtgctcta cggttgaaat ccgtacatca tgctcgttga ccagacacgt
    10501 acagagcatg gtatcacgca cagatttggc atcggccggg aaatctaccg gtggattcaa
    10561 gtcggtgcga cgatagatga ccccggtgtt ggccggcgca gggcgtaacg tcagagtgac
    10621 tttcttgccg gtatgtaaac cgacacccgt cgcctgaacg atacgtttaa gtgtcctttg
    10681 tttgatcatc gtattatctc gccaaattac ctatccaacc gaagtgtact atacattcgg
    10741 cgggccagtt tagcacaaag agcctcgaaa ccaaaattcc agtcaattct taatcagctt
    10801 gcttacgcag gaatgctggg atatccagat aatccggctc tttcgcagtt tgcggcgcat
    10861 tgtcattcac gactttagca accggcttct gctcctgggt cagcggagcc atcccatgct
    10921 gctggtagcg atccatcact ggctgctgaa cctgcttatt ggtcaccaga gtgatttcag
    10981 gacgtttgtc catgccgata cctgtcgcaa caacggttac gcgcagctcg tcattcatat
    11041 ccgggtcaag agaagtaccg ataaccacag tcgcgttgtc ggaagcaaat gcacggatgg
    11101 tgttacctac cgtttcgaac tcatccagac gcaggtcgaa gcccgccgtg atgttaacca
    11161 gcacgccgcg cgcgccagac aggtcgatat cttccagcag cggagaagag atagccattt
    11221 cagcagcttc ttccgcacgg tcttcaccgc tcgccacgcc agaacccatc attgcgtagc
    11281 ccatctcaga cattacggtg cgtacgtctg caaagtccac gttcatcaaa cccggacgag
    11341 taatcagttc agcgatacct tgcacagcgc ctttcagtac atcgttcgct gcgccaaacg
    11401 catccagcag ggagataccg cggcccagaa ctttcagcag tttgtcgttc gggatagtga
    11461 tcagagagtc cacatgcttg gacagttcag tgatcccctg ctccgcgaat gccatacgct
    11521 tcttgccttc aaagttgaaa ggcttagtga cgacagcaac ggtcaggata cccaaatctt
    11581 ttgccacttc agcgacgact ggtgctgcac ctgtaccggt accaccaccc atacccgcag
    11641 caataaagac catgtctgca ccttccagcg ccgcacgcaa tgcatcgcga tcctcatcag
    11701 ccgcattgcg gccaacttct ggattagcgc cagcgcccag tcctttggtg ataccgctac
    11761 cgatttgaat cgtctgtcca accgctgttt tacgcagcgc ttgtgcatcg gtatttaccg
    11821 cgaagaattc aacaccttca atgcgctcgc gcaccatgtg ttcaacagca ttaccgccgc
    11881 cgccgccgac gccgatgact ttaatcaccg cgtcattggt aagttccatt ggttcaaaca
    11941 tagtttctct ccgatttgtg cctgtcgcct gaggccgtaa tcatcgtcgg cctcataaaa
    12001 attaaaactc ttttcgcagc caactattga gtcgcttgat ccacgagcca actgatgctg
    12061 taacacgttt ttctacttca gcttcaccgt taagatgtga ctctttccca tagtgaagca
    12121 atcccaccgc cgtcgaataa tacggctcct gagcataatc cgttaaaccg gtaatgttca
    12181 gcggcgcgcc gatacgcact tgcgtatgaa acacgcgctg agcacaggct gcaagacctt
    12241 cgatctgcgc tgcgccaccg gttaatacaa tgcctgccgc caggtgatgt ttaacccctt
    12301 gttggcgaag cttttcctgc aactgcaata tctcttcgtt gaccaggttg agcagctcgg
    12361 tatagcgcgg ctcgatcacc tctgccaggg tctgacgttg cagactccgt ggcggacgac
    12421 cgcctacgct cggcacttcc acgctctcat cttttccaac gatggaaccc agcgcacaac
    12481 cgtggcgaac tttaatcgct tcggcgtcgc ttggcggcgt gccaaaggcg taagcgatat
    12541 cactggtcac gacattgcca gcataaggaa ttaccttagt gtggcgcaat gccccaccgg
    12601 tataaacggc gatatccatt gtaccacctc cgatatcgac gacgcagaca cccagttcac
    12661 gttcatcttc cgtcaatacc gaataacttg atgccagtcc ggcaaatatc agttggtcaa
    12721 ctttcagccc acaacgttca accgctttga cgatgttttt cgccatatcg ttgtgacatg
    12781 tgatcaggtg cacttttgcc tgcatccgca cgcccgaaag tcctaccgga ttcttgatcc
    12841 cttcctgata gtcaatcgca tactcttgcg ggatcacatg cagcacacga tgttcatcgc
    12901 gcacacgcac cgatttcgcg gtatggacga cgttttccac atcttcttgc gtcacttctt
    12961 cttcagaaat aggcaccata ccaatttcat tctggcagct gatgtgctta ccagaaagcg
    13021 ccagatatac cgaagagatc tgacaatctg ccatcaattc tgcctggtca atggcgcgtt
    13081 gtacgcactt gaccacggat tcgaggtcgt tcaccccgcc tttatccata ccacgtgacg
    13141 ggcagctgcc cacgccaatg atattgacca taccgtcggg cagaacttcc cctactaaag
    13201 cggcaacctt cgcggtacca atctccagtc ctactaccag ttttctgtcc gtcgccttga
    13261 tcattgttgt tctgcctgtg cctgattttg ttgctgagta gattcctctg gcggcaaggg
    13321 cgcccagcct actgccgctc cagagtcata acgcaaatca acgtagctaa tccgtttgcc
    13381 atcggtttgc gccagctgct gtaaaaccgg ataaagttct acaaagcgag ccaaacgttt
    13441 catcgtatcg ccccggccaa gattgagctt aatatcgtta ttcagcgtca actgccagga
    13501 acgccgcgcg gtcatcgccg tttccttcag agtaaatctg tcctttgcca gcatctgccc
    13561 catttcgcga tagccctgca acacttcatt ggcgctgcct tccgggccat acagcattgg
    13621 aagcacctgc ttgctggtgc gatctggcgg cacgctgaag gtgtttcctt ccgcgtctac
    13681 catatgttga tcattccacc gcgcaatcgg cacatattca accagatgaa tcttcaattc
    13741 atcaggccac tgctttctga cgctcacctg cttaatccac ggcaggcgtt gttctatttg
    13801 cgtctggatg atgttgacat cctgggtcat aaaggtaccc ggctcaccca atgccaggat
    13861 cgactgccgg atatcgtcat tacgcgtgta atggcgttca ccggtcaaca ccagctttga
    13921 gagcggcagg cgttgcgcat cttccatcca gcccaacacg acccagccgc tcaccaacac
    13981 tgtcgttaaa acggtcagca ggaaaaggat ccccgccaga cgcgttccat tattgcggcg
    14041 agaagaaacc tcttcttcgc tgtttcgcgt gttcagagca gcctgcgaca tattagtccg
    14101 ccagttccag aattcgtact accaactgcg agaagctcat acctgcctga cgtgccgcca
    14161 tcggcaccag gctgtggctg gtcatacccg gtgaggtatt ggcttccagc agataaaact
    14221 gtccatcgct gtccagcata acgtcaatac gtccccatcc tttgcaacct aacgtcgtcc
    14281 atgctttcag cactaatgcc tgcaaattgg cctcttgtga cgcatccaga cctgcggggc
    14341 agaaatactg tgtctcatca gagagatact tcgcctcata atcatagaag gttccggacg
    14401 gttgaatacg tattgacggt aaaatttctt caccgagtat cgcaaccgtg aactccggcc
    14461 cacttagcca tttttcaatc aatacttctt catcgtgctg aaatgccaat cttaatgcat
    14521 cttgtagagc attttctgct actacttttg acattcccac actggaacct tcgcggctcg
    14581 gcttaacgat aaccggcaaa cccagagcag aaatttctgc taactgctta tcgctcaggc
    14641 ctttttcaaa ctctgcgcgg gttaacgcta cccacggcgc gaccggtaaa ccggcacctt
    14701 gccacagcag tttgctgcgt agtttatcca ttgaaagcgc agatgccatc actccgctgc
    14761 cggtataagg caagcccatc agctcgagca tgccctgcag cgtaccatct tcaccgccgc
    14821 gaccgtgtag cgcgataaac actttctgaa aacccatcga cttcagttgc gtcacgtcga
    14881 cttctttcgg gtcgacagga tacgcatcaa taccaccttc acgcagcccg gctaacaccg
    14941 ctgcgccaga attcagagaa acttcccgct cagcggaagt cccgcccaac aggaccgcga
    15001 ttttatcagt catgttgttc ttcctccgga gtttgcggct tcagtttgat ttcagctaaa
    15061 gaacgggcaa tttttccgat attaccagcc ccctgaacga gaatcaggtc gttaccggtt
    15121 aataccggtg ccagcatctc ggctacctgc gcagggtccg gcaccagaat gggatcaatt
    15181 ttcccacgtc cacgaattgt gcgacacagc gaacggctgt ccgctcccgg aattggcgct
    15241 tcgccagccg gatacacttc cagcatcaac agggtatcaa cctgcgtcag cacattggcg
    15301 aaatcatcat acaagtcgcg cgtacgggta aaacggtgcg gctgaaacag cattaccagg
    15361 tttttatccg gccagcctgc gcgcgccgct ttaatggtgg cgtcaacttc cgtcgggtgg
    15421 tggccgtagt catcgaccag cattgccgta ccgcttttac cattcactgg ctccagcggg
    15481 aattcaccga ggaaatcaaa acggcgacca gtccccagga agctctccag cgcacgcaaa
    15541 atagcctcgt cgtcaattcc ctcttccgta gcaaccgcaa ccgcagctgc ggcgttcagc
    15601 gcgttatgac ggcctggcgc attcagggtg acgcgcatcg gctctttgtc ctggcgcagc
    15661 agcgtaaagt gcccctgcgg gccaatctgc tgataatctt ctacacgcac gtcggcatct
    15721 tcgctgaagc cgtaagtcgt ggtctgacgt cccacacgcg gtaacaattc gcggatcacc
    15781 ggatcatcaa cacacatcac cgcacgaccg taaaacggca ggttgtgcag aaaattaata
    15841 aaagtctgtt ttaaattctc aaagtcgccc tggtaggtat ccatgtggtc ggcttcgata
    15901 ttggtgacaa tcgccaccat cggttgcaga tgcaggaacg atgcatcact ctcatctgct
    15961 tcggcaatca ggtaccgacc atgccccaaa cgcgcatgaa cccccgccgc ttttaccagc
    16021 ccgccgttaa cgaaggttgg gtcgagcccc gcttctgcgt agatgctgga aaccatcgcg
    16081 gtggttgtcg ttttgccgtg cgttcctgca atggcgatgc catgacgaaa acgcattaac
    16141 tcagccagca tttcggcacg acggatcacc ggaatacgcg cttcatgagc tgcgacaatt
    16201 tccgggttat cggcagaaat ggcgctggaa acaacgacca cgctggcatc acgtacgttt
    16261 tccgggcgat ggttgaaata aatcgtcgct cccagattca ttaactgctg cgtgaccgga
    16321 tttggcgcta aatcggaacc actgatctga taaccttcat tggccagaac ttcggcaata
    16381 ccgcccatac cggcaccacc gatgccgaca aaatgtatgt gccgaacgcg acgcatttcg
    16441 ggcacgatgg aacgcagttt tgccaattgt tgtgtattca ttctttacgc cattaacttc
    16501 ttaaattcat acgatgcaaa aggcatcgct acaattacgc ccgggcagcc cggctcactt
    16561 catttgccac tcgctcggtg gcatccggaa tggatgcagc gcgggcgcgt tctgccatgg
    16621 ttaataaggt ttctcgcgac cacccggcca gggtgttggc gacagcatcc acgctaagct
    16681 gtggctgctc gataattttg gctgcgcccg ctttttccag cggtagcgca ttccagtatt
    16741 gctggcggtc tttatgttga aacggcacaa acaacgccgg tagtcctgcc gcggcgattt
    16801 cactcaccgt taaagcaccg gagcggcaaa cgacgacatc cgcccacgca tacgccgccg
    16861 ccatatcatc aataaattcc gtcactttat gctgcggttg ccccgcttcg gcatacgcct
    16921 gttcaacgga ttgttgcgaa cctttaccgc tctgatgcca gatagtgact gaatcaccca
    16981 gtttcgcagc aacctgcggc attgtctggt taagaatgcg tgcgccctgg gaaccaccca
    17041 ctaccagcac acgaaccgga ccttcacgtc cagccaaacg ttgctgcggc aacggcagcg
    17101 ccagcacatc ggtacgcacc gggttaccca ccacttccgc attagggaaa gcgcctggaa
    17161 acgcctgcat cactttggtg gcaatcttcg ccagccattt attggttaag cccgcaatac
    17221 cgttttgttc atgaagtaca accggaatgc ctaacgacca cgcggccaga ccacctggac
    17281 ctgatacata gccgcccata ccgagtacca cgtcaggttt gtacgctttc ataatcgccc
    17341 gcgcctgacg ccaggcgttg aagatacgca gcggcgcagc tatcagtgct tttatacctt
    17401 ttccacgcag accagagata cgaatgaaat caatttcgat gccatgtttt ggcactaagt
    17461 ccgcttccat acggtcggca gtccccagcc agcgaacttg ccaaccctga gccattagat
    17521 ggtgcgcaac cgccagtccc gggaatacat gtccaccggt tccgcctgcc atcaccatta
    17581 atcgctttcc ttgaccactc atcgtgaacc tcgtacaaac gcctgcgctt tctccagacg
    17641 cgtttcataa tcaatacgca acagcatcat gatggctgtc gacataatca gtaagctcga
    17701 accaccgtaa ctgatcagcg gcaatgtcag acctttggtc ggtaacatcc ccgccgccgc
    17761 gcctacgtta accagcgcct ggaagctaaa ccagatgcca atagaacagg cgagaaaacc
    17821 ggaaaaacgg tggtcaattt ctaacgcttt gcggccaatc gacatcgcgc gaaaagcgac
    17881 gaagaatacc attaaaagtg ccagcaccac accgacatac cccagttctt cgccgataat
    17941 ggcgaaaata aagtcagtgt gcgcttccgg cagatactcc agtttttgta ccgagttacc
    18001 taaaccttgt ccccaaagtt cgccgcgacc aaacgccatc agcgattgcg ttaactgata
    18061 gccgctgcca aagggatctt cccacgggtt ccagaatgcg gtaacacggc ggatacggta
    18121 cggttcggcg agtatcagca acacaaccgc tgaaatgccc ataccgataa tggcaatgaa
    18181 ctgccacaat ttcgctcccg ccaggaacaa catcgccagc gtagtcacaa acaccaccac
    18241 caccgtacca aggtctggct gtgccagcag taacactgcc aacaccagaa tcacgcccat
    18301 cggtttcagg aagccgcgca ggttattacg tacttcgtcg cctttacgca ccagatagtt
    18361 ggcgatataa caaaacagcg acagttttgt cagctccgca ggctggatac gcagcaaacc
    18421 gagatcgatc caacgcgatg cccctttaac cgagctcccc actaccagca cgatcatcag
    18481 caggatgata gatccgagca gcatcgtggc actgtagcgt tgccagaact ccatcggcag
    18541 acgcagcgta atgatcgcca gaatgaatgc caggatcaga tagacgccat cacgcttcgc
    18601 gaagaagaac ggatcgttgg ttaagcgttg ccctatgggc attgacgccg aggtcaccat
    18661 gataaagcca atcgccgcaa ggccgaaggt cagccacagt aaggtgcgat cgtacatgat
    18721 caggctgtcg gtatcttttt cccgcgagcc catcacccag ccttttagcg ccgtggagat
    18781 ccagaccagg atactgaatc ctggcaggcg cggcattttc aggcgaggga gagataaacg
    18841 catcaaccta actccttcgc cagacgggca aactcattgc ctcgttgttc aaagttcttg
    18901 aactgatcaa ggctggcaca ggccggggag agcagaacca tatcgcccgg ctgaacacgc
    18961 ggagccagca agcgcatcgc ctgttccata gtttcggttt gttctgccac ttccgggcgt
    19021 agcgccgcca gctgcgcacc gtcacgacca aaacagtaca aacgcacgtt gtcgccattc
    19081 agatagcgcg ccagtggact gaagtccgcc gatttaccat cgccgcccag caacaaatgc
    19141 agcgtgccgt ctacctgcaa gccattcaac gccgcttccg tactgccgac gttagtggct
    19201 ttcgaatcgt taatccagcg tacgccattg tgctccagca ccacttcaaa gcgatgcggc
    19261 agaccggtga aggtggttaa cgctttcagg ctgctggcgc gcggcaaacc agctgcgtct
    19321 gccagtgcca gtgctgccag cgcattggtg tagttatgct gcccggaaag tttcatctct
    19381 ttcacgttca gcactttctc gcctttcact cgcagccagg tttctccctg ctgatgattc
    19441 aggtgatagt cacccatgtt gacgccaaag ctgacgcagc gttcatcagc accgcgaatc
    19501 ggcattgtta aggcatcatc agcattgacg acacaaactt tcgcattttc gtaaatacgc
    19561 aattttgccg cacggtattg ctgcaaacca aacggatagc ggtccatatg atcttcagtc
    19621 acgttcagaa tggtcgccgc caccgcctgt aagctggaag tggtttccag ctggaagctc
    19681 gacagttcca gcacgtacag ttcgcactca gcatcaagca gcatcaacgc tggcaggcca
    19741 atattgccac ccacaccaac gttaaccccc gccgctttcg ccatttcacc cactagcgtg
    19801 gtgaccgtgc ttttgccgtt agaaccggta atcgccacaa tcggtgcttg tgcttcgcgg
    19861 cagaacagct cgatatcgcc aacgatttcg attccggcat cagcggcagc gcttaaggat
    19921 ggatgcgcca gtgcaatacc gggactggcg acaatcagat ctgccgccat cagccattca
    19981 tcattcagac tgcccgtgtg gcgttctacg gcttcgggta atttatccag gccaggcggt
    20041 gtcatacgcg tatccataac gcgcggcgtc acaccgcgag cgaggaaaaa gtccacgcag
    20101 gaaagcccgg tgaggcccag gccgataatg acgacatttt taccctgata atcagccatg
    20161 attaacgtac cttcagcgtt gccagaccaa tcagaaccag catcagcgaa ataatccaga
    20221 aacgcacaat gacgcgcggt tccggccagc ctttcagttc atagtggtga tgaatcggtg
    20281 ccatgcggaa aatacgttgt ccgcgcagtt taaaggagcc gacctgcagg atgacagaaa
    20341 gcgtttctac cacgaacacg ccccccataa tcaccagcag gaattcctga cgtagcagta
    20401 cggcgataat gcctaacgca ccacctaacg ccagcgaacc tacatcgccc ataaagacct
    20461 gcgccggata ggtgttaaac cacaggaagc ccagtcctgc cccgactatc gcggtacaga
    20521 caataaccag ttccccggcg tgtcgcagat acggtatgtg taaatagctg gcaaagttca
    20581 tgttgccggt cgcccacgcc accagcgcaa aaccaccggc gacaaatacg gtcggcataa
    20641 ttgccaggcc gtcgagacca tcggtcaggt ttaccgcgtt gccagtaccc acaatgacga
    20701 agtaagccag cagaatgtag aacagcccca actgcggcat cacatcttta aagaatggga
    20761 ccaccagctg cgttgcgggc gtgtctttgc cggcaaggta cagggcgaag gcgacaccca
    20821 gcgcaatgac cgacatccag aaatacttcc aacgagcgat caaccctttg gtgtctttac
    20881 gcaccacttt gcgataatca tcaacaaagc caataacacc gtaacctacc agcaccacca
    20941 acacgcacca gacgtacgga ttggacgggt aagcccacag cagtacggag atcacaatcg
    21001 ccgtcaggat cataatcccg cccatggtcg gcgtaccgcg cttgctgaag tgtgattcag
    21061 gaccgtcgtt acgcaccacc tgaccaaagg aaagtttttg caaatgagca atcatacgcg
    21121 ggcccatcca caatgagatg aacagcgcgg tcagcaggct gacgatggcg cgaaacgtca
    21181 gataggaaaa gacgttaaag ccggaataat atttgaccaa atgttcggcc agccaaacta
    21241 acatgtccca ttctcctgta aagcgcgtac tacctcttcc atggcggcac tacgtgaacc
    21301 cttaactaaa atcgtaatta cctgttgctc agcaatcagt gatttaagac gcgtaattaa
    21361 cgcagtttta tcagcaaaat gttcgccaac gccgctggcg gtgctgatag catggctttg
    21421 tttacccacg cttaacacgc ggtcaatacc agcagctttt gccgcctcgc ccacctgtac
    21481 atggcaggct tcgctttcag cgcccagttc cgccatatcg cccaccacca gcacgcggta
    21541 gcccggcatt tcagccagta cctgaacagc ggcggtcatt gaaccgacat tggcgttgta
    21601 ggagtcgtcg agcagcaact ggttttctgc cagttggatg gggaacagac ggcctggaac
    21661 agctttcaga tttgccagcc ccgctttgat agcatcaagc gttgcgccca cggacatgga
    21721 gagcgcagcg gctgccagcg cattcgcaat attgtgacgc cccggcaacg gcagcagaac
    21781 atcgacgcta ccggttgggg tttgtagggt aaattccgta ccgtgcgagg tcacatggat
    21841 attggtggcg gtgaaatcgc tgttggcggc attgggtgag aaacgccaca ctttgcgtga
    21901 gccaattacg ctctgccagt tcagccagtc gttgttgtcg gcgttcataa tggcgatacc
    21961 gttttccggc aggccgctaa agatttcacc tttcgctttc gcgacacccg caagcgagcc
    22021 aaaaccttcc agatgcgccg ctgccaggtt gttgaccagc gcagcttccg ggcgagtcag
    22081 actcacagtc caggctattt cgccctgatg gttcgcgcca agttcaataa ctgcgtaatc
    22141 gtattccggc gttaagcgca acaacgtcat cggtacaccg atgtcgttgt tgagattgcc
    22201 tgccgtataa agcgtgttgc cgcactggct taaaatcgcc gccgtcatct ctttaacgga
    22261 ggttttgccg gaggaacctg tcagagcaac cacgcgcgcc ggaacttgct ggcgaaccca
    22321 tgcagccagt tcaccaaacg ccagacgcgt atccttgacg attaactgcg gcaggtcgat
    22381 atccagcgga cggctgacca gtagtgcgcc cgcgccgcca gctttcgcct ggtcggcaaa
    22441 atcgtgggca tcaaaacgtt cgcctttcag ggcaacaaac aggcagcccg gcgtcagttt
    22501 tcgggtatca gtggttacag catcaagggt gatatctgca ccttgcagtt caccgttgag
    22561 aatgtcggta agttggctaa gggttacgct aatcatgcaa tcacccccag cagacgcgcc
    22621 accgtgacgc gatcggagta gtccagacgc tgattgccaa caatctggta atcttcatgg
    22681 cctttgcccg cgaccagcac cacatcgttc tctttagcct gcataacggc gcaagtcacc
    22741 gcttcagcac ggccttccat cactttggca tgtccggcat ctaacattcc cgccagaata
    22801 tcgttgatga tggcacgcgg ttcttcggta cgcgggttat cgtccgtcac caccgccacg
    22861 tcagcaaact cttcggcaat tgcgcccatc agtggacgct tacctttatc gcgatcgcca
    22921 ccacagccaa agacacacca cagcttgccc gcacagtgca gacgcgccgc ctgtaaggct
    22981 ttttccagtg catccggcgt atgcgcgtaa tccaccacca ccgtcggttt gcctggcaca
    23041 gtgaacactt ccatacgtcc gcaaaccggt tgcagacgcg cggcggtttt caacagatca
    23101 gccaatggat agccgagtgc caacagtgtc gccagcgcga gcagcaggtt gctgacgtta
    23161 aaagcgccta tcagatggct ttcaatttcg ccatcgcccc aacttgagct aaagcgaatc
    23221 gtcacaccgc tgtcgtgata gttcacgtcg atcactttta accagcgtcc gtgacagttc
    23281 ggattaatat gatcttccat tgataccgca accgcgtccg gcagctttgc cagccaacgg
    23341 cggcccactt catcgtcggc gttaataatc gcctgaccgc aatgatgttc agaataaagc
    23401 agccatttcg cagcttcgta gtgttccata tcaccatgat aatcaaggtg atcgcggctt
    23461 aagttggtaa agaccgacgc cgcaaatttc aatgccgcca cacggtgctg taccagcccg
    23521 tgggaggaaa cttccattgc gcaaaacgtc gcgccctgct ccaccagccc cgccagctca
    23581 tgctgaacat cgactgccga accggtggta ttttctgtcg ggatcacttt ccccagcaga
    23641 ccgttaccaa cggtgcccat taccgcgctg gtttcgccaa gcagttggct ccactgcgcc
    23701 agcagctggg tagtcgtggt tttgccgttg gtgcccgtta cgcctacgag acgtaaattg
    23761 tcagagggtt catggtaaaa gcggcccgcc agtgcagata aacgctcgtt gagctggctg
    23821 agatagatga ccggtacgcc gtgcatttca cggatttcac cgtcggtcgc ctcatctttc
    23881 gcctctgcaa taatggcagc cacaccttgc gctatcgcct gcgggatata tcgacgcccg
    23941 tccgcctgat gacctactac agctacaaag agatcgcccg ccgcagccac acggctgtcg
    24001 agtgtcatct ctcgcagtgc tcgcgaaggt gcgtctggca cccacggagc aagaaggtcg
    24061 cgcaaattac gatctgccac ctgtcccctc gccttgatta atcacaaatt catttttatc
    24121 gcccgttgtc agcgcatccg gctcgatgtt catggtacgc aatacgccgc ccatgatggc
    24181 accaaagacc ggcgcggaaa cggcgccgcc gtagtattta cccgcctgcg gatcgttgat
    24241 aacaacaacc agcgcgaagc gcggctgact cgcaggcgca acgcctgcgg tataagcaat
    24301 atatttattg atgtagcgac cgtccggccc gacctttttc gcggtaccgg ttttaatggc
    24361 gatacgatag cctttaatcg ccgccttcac gccgccgccg cccggtagcg ccacgctttc
    24421 catcatatgc accacggtgc ggacaatgga ttccgggaag acacgttcac cgggaaccgg
    24481 ggggtcaact ttggtaatcg acagtgggcg ataaatgccg tagctgccga tagttgcgta
    24541 gactcgcgct aactgtaacg gtgttaccat tagcccgtag ccgaaagaga aggtggccct
    24601 ctctatgtca gaccaccgtt gtttttgagg atataagcca ctgcgttctc cgaccaaccc
    24661 caaattggtc gcttttccca gtccaaaacg tgagtaagta tctactaacg ctgaggacgg
    24721 catcgctaac gccagcttgg aaacaccgac gttactcgac ttctgtaata ccccggtcag
    24781 ggttaattcg ctgtagcgtg ccacgtcttt gatttcgtgg ccgttaattc gataaggaat
    24841 ggtattgagt accgagtttt cccgcaccac gccacgttgc aacgcggtca tcaccaccat
    24901 cggtttaacc gttgagcccg gttcaaacac gtcggtgatg gtacggttac gcatcgcctc
    24961 tttcggcgtg ccgctcagat tgttagggtt gtatgacggg ctgttagcca tcgccagcac
    25021 ttcacctgtg ttgacatcca ccagcacggc gctaccagat tcagccttgt taaaggccac
    25081 cgcgttgttc agttcgcgat aaaccagcgc ctgcaggcgt tcatcaatac tcagcgccag
    25141 gttgtgcgct gcctggctgt cagtagaaga aatatcttca attacgcgac catagcggtc
    25201 tttacgcaca atgcgctcac ccggctgccc ggtaagccat ttatcgaaac tcttctcaac
    25261 gccctcaatc ccttgactat cgacgttagt aaagccaatg aggtgagcag tcacttcgcc
    25321 ggatggataa taacggcgag attcttcacg cagatgaata cccggcagtt tcagtttttt
    25381 gatgtagtcc gccatgtcag ggtttacctg acgcgccaga taaataaagc gccctttcgg
    25441 gttggcgtta atgcgggcag aaagctgatc cagcggaata ttgagcgcgt tagccagcgc
    25501 cttccagcgg tcaccgacgc tgataccgcc agcgtcatgc acttctttcg ggtcagccca
    25561 aatcgctttt accggcacgc tcaccgctaa cgggcgacca gaacggtcag taatcatgcc
    25621 gcgggaggtg gaaacttgct gaacgcgaag agaacgcatg tcgccctctt tcaccagcat
    25681 atccggggag ataacttgta accacgctac gcgtccgagc agaaaagcca gcgccaggag
    25741 aatacagccg cataacaacg caaaacgcca actaataaag ttggcatgtt cttcctgacg
    25801 ttttggtttc tgcgttttcg ccgctgcttt catgcgtcgc gtttatcctt atttttgcac
    25861 tacgatattt tcttgtgacg gatcaacatg ctgcatttgc agcttttccg tggcgatcct
    25921 ttccacccgg ctatggtcgc cgagcgcatt ctcttcaagg atcaggttgc gccattcaat
    25981 gtctaaagca tctcgctcca gcaccagttg ttcgcgctga gcggtcagta aacgggtatg
    26041 gtgcgccgtg gttaccacgg tcaccgccgt caaaataatg caaatgaaca ggcagagtgg
    26101 cagcttccca aatcgcaaaa gatcgtcacc gataacacca ggcaatgcat ggcgctcgtg
    26161 gcttcccatc gatcctttaa ctttgcttag agcttctgtc actctgctga tcatgcgttc
    26221 gtcctctctg cgatacgcag aactgaacta cgggcacgag gattctcagc cacctcttct
    26281 tcgcccggca ttaacttgcc taatgctcgc agctgacggc cacccagttt tttgagctgc
    26341 tcttcagtca tcggtaaccc tgccggaact tgcggaccgc ggctgttttc acgcataaaa
    26401 cgtttcacaa tacggtcttc cagcgagtgg aagctgatga tcgaaagccg cccacccggg
    26461 gccagcacgt tgagcgagct ttttagcgcc tgctctatct cctccagttc actgtttacc
    26521 caaatgcgca ccgcctggaa ggtacgggtc gcgggatgtt taaacttatc tttcaccggc
    26581 gttgcagcag ccacgacttc cgccagttct ttggtgcggg tcatcggctg ttcgcggtta
    26641 cgctcgacaa tggcgcgggc aatgcgtttg gcaaaacgct cttcaccata ggttttcaat
    26701 acccaggcga tatcggcttc ttctgcggtt tgtagccatt cagcggctga ctgcccacgg
    26761 gttgggtcca tacgcatgtc cagcggacca tcgcgcataa aggaaaagcc acgttcagca
    26821 tcatcaagtt gcggtgaaga gacgccaaga tcgaggagaa tgccgtcgat cttgccgata
    26881 agatcgcgct cggcaacgta ttcgcccagc gcggagaaag gtccgtggat gatggagaag
    26941 cgcggatcat caatagtctt cgcaacggcg atagcctgcg ggtcgcgatc gatcgccagc
    27001 aaacgcccct cttcgccaag ctgcgagagg atcagacgtg agtgaccacc gcgaccaaaa
    27061 gtcccatcaa tgtagatgcc atcaggacgg atattgaggc cattaacggc ttcatccagc
    27121 agcaccgtag tatgtttata gttttccatc attttataga gacaagtcct gcagtcgctc
    27181 cgataagtct ccagtagcca actgctctgc gtcgatatct tccttgacct gttgatgcca
    27241 ggttgtttca tcccacagct caaacttgtt gaactgtcca accagcatca cttcttttgt
    27301 cagcccggca tgttgccgca gtactggcgc gattaacaat cgacctgcgc catccatctg
    27361 acattcgctg gcatgaccta acagtaggcg ctgcacacgg cgctcaactg ggttcatgct
    27421 cgacagacgc gataattttt gctcgataat ttcccattca ggcagggggt aaagcagcag
    27481 gcacgggtga tgaatgtcaa tggtgcaaac catttgaccg gcagcgttct caagcagctg
    27541 ttcccgataa cgggtaggca ctgataagcg ccctttgctg tcgagattga ctaacgttgc
    27601 tccccggaac atgccagcct caccccttat tcccactttg ccccacaatt tcccactgaa
    27661 aggagtttac ggagcggagg aaaagcttgt caagccagtc acaaggcata ccgaggtaaa
    27721 aaacccgatg tttacggcta atatcagcgt tgtttatttt acgtccaacg cgcgaagcaa
    27781 attaacgtta tgaatagctg taagaaaaaa tccctcatcg tcatcactgt tttaaaatga
    27841 ctcactattt tccacagcaa atataaagtg tcagtttgcg acgcgagcgg cattttagga
    27901 catatcttcc ccggttaaca gtccttgtta cgtctgtgtg gcgcttgatg ccagcgggtt
    27961 tgcgcaaaag caaagaaaag tgtttgttaa atcggcaatt catcgccgca tgtaacaaaa
    28021 taatgcaaaa acgcgctgtc gatatcactt aattattgtg aatttcagct ggtgagttaa
    28081 ttaacgggcg ggtttaaaac catttatcaa taatagtcag gaatagtctt atttacttta
    28141 agcatattga tgtccagtcc cgtactctac gcgccagagg gaaattcacc tggcgcgtat
    28201 ttttgttcgc ggcttagcta cggctgagca caccgcggcg atagagatta cgtttaatgc
    28261 gcgttaaacc aggttttggc ttacgcggtt cgtccaggct tgccaggaca atctccagca
    28321 cacgctctgc gacatcgcgg tgacgttgag ccactgccag caccgggcac tgtaagaagt
    28381 cgagcagttc gttatcgcca aaggtggcaa ttgccaggtc agaaggcagt ttaccatcgc
    28441 gacgtagcgt gacatccatc accccttgca acaacgcaaa cgacgttgtg aacagcgcct
    28501 gcggcatcag atgcgtttcc agccattttt cgaataactg ggcagccgcc tcccgctcat
    28561 agctgttggc atacaggaaa tgcacttcgc gcggatcatc tttccaggca gtacggaaac
    28621 cttgttcacg caggaagctg acagaaagct ccggcagcgc gccaagataa agcaccgtct
    28681 cggcgggaaa cttacgtaac tcttccgcca gcatttcggc atcatcctga tcggcaccaa
    28741 ccacgctggt gaaatgttca cgatcgaggg cgcggtccag cgcgacaatc gggaacgggg
    28801 cgttagccca gcgttgataa aaaggatgct caggtggcaa cgacgtcgaa acaataatgg
    28861 catcaacctg acgctgtaaa aggtgctcaa tgcagcgcat ttcgttgtct ggctgatctt
    28921 ctgagcaggc aatcagcagt tgataacccc gttgccgcgc ctggcgctca agatagttag
    28981 cgatgcgggt atagctggtg ttctccagat cggggatcac aagaccaata gaacgtgtgc
    29041 gtccagcacg aagcccagct gccacggcgt tcgggtggta attgtgctca cgcaccacag
    29101 ccatgacttt ttcaacggtt ttgtcgctca cacggtattg cttcgctttg ccgttaataa
    29161 catagcttgc agtggtccgc gacactcccg ccagccgagc gatttcatcc agtttcacaa
    29221 ttgccccttg cgtaaaatgt aaaaaccata gccattgtac tggtatgggt taaattcatt
    29281 aacatctaag cgcagaaaat taactgcggc aactgctttt attccaggtt cccactgatt
    29341 tcgcaaaaaa agcccagcgt gacatgttgg gctgcaaatt gcacactgag atcattctca
    29401 acgcattatt ttatcgccgc gcgaaagtcc gaccacacca gagcgagcaa cttccacaat
    29461 tttcgccacc tcgcgaatcg atgctaaaaa tgcatcaagc ttatcgctgg tgcctgctaa
    29521 ttgaacggta taaagcgagg gggtgacatc gataatttgc ccacggaata tttccgtatt
    29581 acgtttcact tcgtcacgcc cgtaaccgct ggcctgaatt ttcaccagca tgatttcccg
    29641 ctcaacatgc gcgccctgcc ccaactcact cacgcgcaag acatcgacca gcttgtgtaa
    29701 ttgcttttcg atctgctcaa gtactttttc atcgcccacg gtctggatgg tcatacgcga
    29761 taatgtcgga tcgtcggttg gcgcaacggt caggctttca atgttgtagc ctcgctggga
    29821 aaaaaggcca atcacgcggg ataacgcgcc tgattcattt tcgagtaaga ctgataatat
    29881 ccggcgcata atcaggttct ctccgttttg cttaaccaca tttcatccat tccgcccccg
    29941 cgaatctgca tcgggtagac gtgctcactg ccatcgacgg taacatcaac aaacaccagg
    30001 cgattattgc gcacctgttc cagcgcctcg ctaagtttgc tttccagctc atgcggatga
    30061 gagatctgga tcccgacgtg accataggct tccgccagac ggacgaaatc gggtagtgat
    30121 tgcatataag attgtgaatg acggccggaa tagatcatgt cctgccactg tttcaccatc
    30181 cccagatagc ggttattgag attcaccacc agtacgggca actcgtattg caacgcggta
    30241 gacagttcct gaatgttcat ctgaatactg ccgtcgccag tgacgcaaac cacggtttct
    30301 tctggcaacg ccattttgac gcccagtgcc gcaggtaaac caaaacccat cgtgccgagg
    30361 ccaccggaat tgatccagcg acgcggtttg tcgaatggat aataaagtgc agcaaacatc
    30421 tggtgctgcc cgacatcgga cgtcacgtaa gcgtctccct tcgtcaaccg ccaaagagtc
    30481 tcgatcaccg cctgcggttt aatcttttca ctgtgagtgt catatttcag gcactgacga
    30541 gcgcgccact gttcaatttg ctgccaccag tcgcggatct catccagtgg ttgatgggcg
    30601 gattcttgcg acaagagttc aagcatttgt tcgaggacct ggcgagcatc ccccacaatc
    30661 gggatatccg cagtcacggt tttagaaatg gaaataggat caatatcgat atgcagaaca
    30721 gtggcatttg ggcagtactt tgccagattg ttcgtcgttc ggtcatcaaa tcgtaccccg
    30781 acggcgaaaa tcacatccgc gttatgcatc gtcatattgg cttcgtaggt accgtgcatt
    30841 cccagcatgc ccagtgcctg acgatgcgtt gccggaaacg cccccagccc catcaatgag
    30901 gaaacaacgg gcagattcaa cgcctccacc gtttctttca actgctgatg gcagcccgcc
    30961 atgattgccc caccgcctac gtagacaacc ggtttttttg ccactaccag cgtttgcaga
    31021 gcacgcttaa tttgcccttt atgtccggta gtagtgggat tgtaagaacg catactgacc
    31081 gactccggcc agacataggg taatttgttc gccggattaa gaatatcttt cggtaaatca
    31141 acgactactg gtccaggacg accacttgcc gccagccaga aagccttttt cagcacctgc
    31201 ggaatgtctt ccgtttgctt aaccagaaaa ctgtgtttaa ccactggtcg cgaaatcccc
    31261 accatgtcgc actcctgaaa ggcatcgtaa cctatcaacg aggtcgctac ctgcccggaa
    31321 aggacaacta atggaatgga atccatataa gcggtggcga tgccggtaat cgcattggtc
    31381 gcccctggac ccgacgttac cagcacgacg ccgacttccc cggtcgcgcg cgccagacca
    31441 tcggccatat gcactgccgc ctgctcatga cgaactaaaa catgatcgat accacccacg
    31501 gtatgcaatg catcgtaaat atcaaggact gcgcctccag gataaccgaa tacttgttta
    31561 acgccctgat cgataagcga tcggacgacc atctcggctc cagacaacat ctccatggcc
    31621 tgcctcactg tttgatggaa aaaatgtgta aaacacatta accgtccgat cttgcttaag
    31681 caattggcag ccagagacat aaacgcaggg taataagaat aaacaggagg aaagatgaaa
    31741 agaaacgata aaatcctcca ttacgaagga ttaaattgat aataaatcgg ctgaatccca
    31801 caacttacag caatttctct atttttttaa ttaatcctaa aatagagagc ccacacattc
    31861 agaataaaat tctacattca ataaaaaatg cagaataaac cagacattca tgtctgacct
    31921 attctgcatt cagttagcgt ttgcaaattg agactaattg ttcttccatc cactgatggc
    31981 ctttatcgcg cccggcagct tcatgccatg agagataaca ggttctgctg ttttgtttta
    32041 acggcagcgg taatacctgt aattctaagg attcagcgaa ctcttcagcc agccaacgcg
    32101 gcgcaatagc gaccaaatgc gtttgcgaca ccacgctaag tacgctcatc attgccatgc
    32161 cctgatacgc gatactggct tgcttatcta ccgtgtcata ccaaggttga ctaaatgacg
    32221 cgaaacgatc gagcgaaacc gccgcatgtt gttcgttata aacatcatgt ttcagtaacg
    32281 ggcctttaat tgttggatga tttttgctgg ctaccagcac catttcatct ttaaataatg
    32341 gcacgctggt aaattcagga cgatggaagt cttcataact aatcacaaac tccgtttcct
    32401 gataacgcag ctgatgttca gtgttctgat ttaatgaaga cttgaacata acatgtatat
    32461 ttggcgcaat ctgctcaatg tgattataaa tctgcgaggt cagaatgctg tctaacgggc
    32521 tgcaaacaca aagatgaaat acacgttcac tactcgcggg ttcaaaacct gaaccaggca
    32581 attcattttg tactagttgc aatgcctggc gaactgaacc aaaaagttga aatgcgcgag
    32641 cagtcggttg aataccacgg ccataacgaa caaaaagctc gtcattaaac atcaccttca
    32701 ggcgtgcaac agcgttactg accgcaggtt gcgacatacc cagaacatga gcggcacggg
    32761 taatgttttg ctcctgcatc acggcatcga aaacggttaa taagttgaga tcgaccatgc
    32821 gtagctgtgg cttgcttaac tccgccgtct ctgaatgatc tgtttgtacc tctggcatac
    32881 ttaactccac tgtcacactt aactcccttt cccttattgg aatgcagaga aataatcctg
    32941 aagaatatga tttatc
//
LOCUS       DAJTMC010000028        32487 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.028, whole genome shotgun sequence.
ACCESSION   DAJTMC010000028 DAJTMC010000000
VERSION     DAJTMC010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32487
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.028"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            197..991
                     /gene="panB"
                     /locus_tag="OO130_001164"
     CDS             197..991
                     /gene="panB"
                     /locus_tag="OO130_001164"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HCR5364655.1"
                     /translation="MKPTTIASLQKCKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTASVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAG
                     DQLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            1003..1854
                     /gene="panC"
                     /locus_tag="OO130_001165"
     CDS             1003..1854
                     /gene="panC"
                     /locus_tag="OO130_001165"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HCR5364656.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIIAIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKIVELA"
     gene            complement(2146..3042)
                     /gene="rpnC"
                     /locus_tag="OO130_001166"
     CDS             complement(2146..3042)
                     /gene="rpnC"
                     /locus_tag="OO130_001166"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HCR5364657.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQGKPDKKMAFRMMRYSIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDIFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGESMMTLAQWFEEKGIEKGIQQGRQEERQEFAQRFLSKG
                     MSREDVAEMTNLSLAEIDRLIN"
     gene            3316..3696
                     /gene="panD"
                     /locus_tag="OO130_001167"
     CDS             3316..3696
                     /gene="panD"
                     /locus_tag="OO130_001167"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HCR5364658.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(3700..4900)
                     /locus_tag="OO130_001168"
                     /pseudo
     CDS             complement(3700..4900)
                     /locus_tag="OO130_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001727225.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
     gene            complement(4995..5435)
                     /locus_tag="OO130_001169"
     CDS             complement(4995..5435)
                     /locus_tag="OO130_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR5364659.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRERIVELGAPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(5540..6310)
                     /locus_tag="OO130_001170"
     CDS             complement(5540..6310)
                     /locus_tag="OO130_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5364660.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLATTFGVLVVFIVVFYLICWSLIQRGRGLRS"
     gene            complement(6307..7233)
                     /locus_tag="OO130_001171"
     CDS             complement(6307..7233)
                     /locus_tag="OO130_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5364661.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKLETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            7342..8004
                     /gene="can"
                     /locus_tag="OO130_001172"
     CDS             7342..8004
                     /gene="can"
                     /locus_tag="OO130_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HCR5364662.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPHFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(8045..8581)
                     /gene="hpt"
                     /locus_tag="OO130_001173"
     CDS             complement(8045..8581)
                     /gene="hpt"
                     /locus_tag="OO130_001173"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HCR5364663.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            8787..11177
                     /gene="gcd"
                     /locus_tag="OO130_001174"
     CDS             8787..11177
                     /gene="gcd"
                     /locus_tag="OO130_001174"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HCR5364664.1"
                     /translation="MAINNTGSRRLLVTLTVLFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGSILTWAGFNDPQEINGTLSADATPTEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHNLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPKLKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPKKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(11224..12774)
                     /gene="cueO"
                     /locus_tag="OO130_001175"
     CDS             complement(11224..12774)
                     /gene="cueO"
                     /locus_tag="OO130_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HCR5364665.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLTEETTLHW
                     HGLEVPGEVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFNADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHDAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            12940..13287
                     /locus_tag="OO130_001176"
     CDS             12940..13287
                     /locus_tag="OO130_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HCR5364666.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            13393..14259
                     /gene="speE"
                     /locus_tag="OO130_001177"
     CDS             13393..14259
                     /gene="speE"
                     /locus_tag="OO130_001177"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HCR5364667.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPSGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            14275..15069
                     /gene="speD"
                     /locus_tag="OO130_001178"
     CDS             14275..15069
                     /gene="speD"
                     /locus_tag="OO130_001178"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HCR5364668.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(15106..15468)
                     /locus_tag="OO130_001179"
     CDS             complement(15106..15468)
                     /locus_tag="OO130_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HCR5364669.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(15643..18240)
                     /gene="acnB"
                     /locus_tag="OO130_001180"
     CDS             complement(15643..18240)
                     /gene="acnB"
                     /locus_tag="OO130_001180"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HCR5364670.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGLLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFMNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            18595..20448
                     /locus_tag="OO130_001181"
     CDS             18595..20448
                     /locus_tag="OO130_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HCR5364671.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASAGLYIKSRTVEAAVETQSTQ
                     LAVSDAAAVTLPATVSAPPVTPAVVKSAFSTAQIDQWVAPVALYPDALLSQVLMASTY
                     PANVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSNEQKVITTTKKAVPVKQTVTAPVIPSN
                     TVLTASPVITEPATTVISIEPANPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FIDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDGNGWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAASQFQQRTHAAPVITRDTQRQAAAQRFNEAEHYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVRERMQTNPQIQQRREAARERIQPASPEQRQ
                     AVREKMQTNPQIQQRRDAARERIQSASPEQRQVFKEKVQQRPLNQQQRDNARQRVQSA
                     SPEQRQVFRERVQESRPQRLNDSNRTARLNNDQRSAVRERLSERGARRLER"
     gene            complement(20519..21943)
                     /gene="lpdA"
                     /locus_tag="OO130_001182"
     CDS             complement(20519..21943)
                     /gene="lpdA"
                     /locus_tag="OO130_001182"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HCR5364672.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(22268..24160)
                     /gene="aceF"
                     /locus_tag="OO130_001183"
     CDS             complement(22268..24160)
                     /gene="aceF"
                     /locus_tag="OO130_001183"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HCR5364673.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAAPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(24175..26838)
                     /gene="aceE"
                     /locus_tag="OO130_001184"
     CDS             complement(24175..26838)
                     /gene="aceE"
                     /locus_tag="OO130_001184"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HCR5364674.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHSI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(26999..27763)
                     /gene="pdhR"
                     /locus_tag="OO130_001185"
     CDS             complement(26999..27763)
                     /gene="pdhR"
                     /locus_tag="OO130_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HCR5364675.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            28304..29677
                     /gene="aroP"
                     /locus_tag="OO130_001186"
     CDS             28304..29677
                     /gene="aroP"
                     /locus_tag="OO130_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HCR5364676.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNSGPQATVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(29720..30574)
                     /gene="ampE"
                     /locus_tag="OO130_001187"
     CDS             complement(29720..30574)
                     /gene="ampE"
                     /locus_tag="OO130_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HCR5364677.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARVTMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGIWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(30571..31122)
                     /gene="ampD"
                     /locus_tag="OO130_001188"
     CDS             complement(30571..31122)
                     /gene="ampD"
                     /locus_tag="OO130_001188"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HCR5364678.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            31210..32103
                     /gene="nadC"
                     /locus_tag="OO130_001189"
     CDS             31210..32103
                     /gene="nadC"
                     /locus_tag="OO130_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HCR5364679.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            32306..>32487
                     /locus_tag="OO130_001190"
     CDS             32306..>32487
                     /locus_tag="OO130_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCR5364680.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHG"
BASE COUNT         7831 a         8833 c         8004 g         7819 t
ORIGIN      
        1 gccacgctac aacaggatgg aactatacca atagaagctg gtgagtttaa agccaccagt
       61 actttccagg taacctaccc ttaataagtc caccgcaccg ccatcatctg gcggtgcggt
      121 aattgataaa tcattgcccc caccaatgtg actgataaaa tgccaggcaa cacgacatca
      181 tttatcagga cacgttatga aaccgaccac cattgcttca cttcagaaat gtaagcagga
      241 taaaaagcgt ttcgcgacca tcaccgctta cgactacagc ttcgcgaaac tatttgctga
      301 tgaaggactt aacgtcatgc tggtaggcga ctcgctgggc atgacggttc agggtcacga
      361 ctccaccctg ccggtgaccg ttgccgatat cgcctaccac actgcctccg tacgtcgtgg
      421 tgcgccaaac tgcctgctgt tggctgacct gccgtttatg gcgtatgcca cgccggaaca
      481 agcctttgaa aacgccgcaa cggttatgcg tgccggtgcc aatatggtca aaattgaggg
      541 cggtgagtgg ctggtcgaaa ccgtaaaaat gctgaccgaa cgtgccgttc ctgtatgtgg
      601 tcacttaggt ttaaccccac agtcagtgaa tattttcggt ggctacaaag ttcaggggcg
      661 cggcgatgaa gcgggcgatc aactgctcag cgatgcatta gccttagaag ccgctggggc
      721 acagctgctg gtgctggaat gcgtgccggt tgaactggca aaacgtatta ccgaagcact
      781 ggcgatcccg gttattggca ttggcgcagg caacgtcact gacgggcaga tcctcgtgat
      841 gcacgacgcc ttcggcatta ccggcggtca cattcctaaa ttcgctaaaa atttcctcgc
      901 cgaaacgggc gacatccgcg cggctgtgcg gcagtatatg gctgaagtgg agtccggcgt
      961 ttatccgggc gaagaacaca gtttccatta aggagtcacg ttgtgttaat tatcgaaacc
     1021 ctgccgctgc tgcgtcagca aattcgccgc ctgcgtatgg aaggcaagcg cgtggcgctg
     1081 gtgcctacta tgggtaatct gcacgatggc catatgaagc tggtcgacga agccaaagcc
     1141 cgcgccgatg tggtcgtcgt cagtattttc gttaacccga tgcagttcga ccgcccggaa
     1201 gatctggcac gttatccacg gaccttgcag gaggactgcg agaagctaaa caaacgtaaa
     1261 gtggatttag ttttcgcccc ttcggtaaaa gagatctacc cgaacggtac tgaaacccat
     1321 acttacgttg acgttcccgg gctttcgacc atgctggaag gcgcgagccg tccggggcat
     1381 tttcgcggcg tttcgactat cgtcagcaag ctgtttaacc tggtccagcc ggacatcgcc
     1441 tgcttcggtg aaaaggactt tcagcaactg gcgctaatcc gcaaaatggt tgccgatatg
     1501 ggcttcgata ttgaaattgt cggcgtcccg attatgcgcg ccaaagatgg ccttgcgctc
     1561 agttcccgta atggttatct gacggcggaa caacgcaaaa ttgcgcccgg tctgtacaaa
     1621 gttttaagtt caattgccga caaattgcag gctggcgagc gggatcttga tgaaattatt
     1681 gccattgcag ggcaagaact gaatgaaaaa ggcttccgcg ccgatgatat tcagattcgc
     1741 gatgccgaca cgttgctgga agtctctgaa accagcaaac gagctgtaat tctggtagcc
     1801 gcctggcttg gcgatgcccg cctgatcgac aacaaaatcg tcgaactggc gtaatgcttt
     1861 tactctaaat aattcaagtt gcaggaaggc ggcaagcgag tgacaaattc gtcaggaacg
     1921 aatttgccca gccgcaggct ggccttcggt gagagacatg gatgtctctc attaatcccc
     1981 aggagcttac ataagtaagt gactggggtt agcgaacgca gacacagcac atgcaattta
     2041 aagtatgacg agtataactg gcgctacggc tgatggcgcc aattattaat ttgccccacg
     2101 tcatggcgat ggcgtctgta agcaattatt cataacgcag acaacttagt taatcagcct
     2161 atcaatctca gcaagagata aatttgtcat ctctgcaacg tcttcccgag acatcccttt
     2221 actcagaaaa cgctgggcga attcttgcct ttcctcctgt cttccctgct gaatcccctt
     2281 ctcaatccct ttctcttcaa accactgcgc cagcgtcatc atagactccc ctcccgtttc
     2341 cctgtctctc agcacaccat aaaacaaatc cgcttgttca gtatgaccgc gttgcagcat
     2401 atagttttgc atggcaacta actgacttcc gctagtgtac ccttcgtcaa tcagcgtgac
     2461 cagttgctcc agcaataaca ttaagtcgcg ctggcgaata tgtttttgca gtagttcgag
     2521 aatcgcaatc cgccgatgtt gcatgatttc gtcatccggt gtgatggtga tatccaccag
     2581 cgggaaagga ctgttataga cgcgtcgtgc cagctccggc gagtaaaata tatcaaacca
     2641 gcacattgag agcggataag gtgtggcctc gccctgataa aacagtatcg gcaccaccag
     2701 cggcagctta tcgtgatccg cctccagatg ccggtgcatg gcagcaatag aataacgcat
     2761 catgcgaaag gccattttct tatccggctt gccttggtgt tcaatcacaa catgcagata
     2821 accgggattg ccctgcattt gcacggaata gagcacgtcc gtgctgtgtc ctttcaggct
     2881 ctcttcaatg aaactccccg actctaaatg aagcgtgttg aggtcacaaa gttctcgtaa
     2941 ttccactggc aaatgtatct ccagaaagtc gcgagccgtc tccgcgtgca ttaaaaattg
     3001 tttaaacact gcatcatgcg gtgtggtact cggtgcatcc atcttccctg ccttccgtgt
     3061 tgtcttgcgt gaaggcagta tccagtcaag gcgtgccatc gtcagccacc tctttagtgt
     3121 ttaccgagca gcgttcagag aaatttcaat tggttacagc cgttacatgg cacgcttcgg
     3181 cacggtacgc aaactgctga tgtaaatgcc gtcagatgag tagacactaa acaaaaatcg
     3241 ggcaatactg cgtgagaatt tcctcaaagc aggcatcgcc tgcttcgtta acgacagggt
     3301 agaaaggtag aagttatgat tcgcacgatg ctgcagggca aactccaccg cgtgaaagtg
     3361 actcatgcgg acctgcacta tgaaggttct tgcgccattg accaggattt tcttgacgca
     3421 gccggtattc tcgaaaacga agccattgat atctggaatg tcaccaacgg caagcgtttc
     3481 tccacttatg ccatcgcggc agaacgcggt tcgagaatta tttctgttaa cggtgcggcg
     3541 gcccactgcg ccagtgtcgg cgatattgtc atcatcgcca gcttcgttac catgccagat
     3601 gaagaagctc gcacctggcg acccaacgtc gcctattttg aaggcgacaa tgaaatgaaa
     3661 cgtaccgcga aagcgattcc ggtacaggtt gcttgattgt tatccctgcg gctggttact
     3721 caccagccgc gacatcgtct ccagcgaatc cgttcttaag atataaagtc gttttagtaa
     3781 caacggatta tcccccggtt ttactttgcc tttcatggtt gtcaccgcca ggtgaaatcc
     3841 ggcatcgttt gctgccttca cggctttgtc attaaatccg ccaaacggat acgaaagata
     3901 ccagacatgc ggattaaatt gcgccagagc gcggcgtgaa cgtgcaaaat caaacagaat
     3961 attgtgttca ctacggctcg gtaatatggg gcggcgataa ccatccaccc gatgcaaaaa
     4021 atgggtatgt gactggaaat caaaaacatc gcgaatttcg ttaagttcag aaacgctcat
     4081 aaattgcagc gattttgggt cccacttctg cgggtgacgt ttgatgcgcg aggtaacaat
     4141 aaacgccgtc gccttcatgc catattgttt caacacggga tacgcatagc ggctcaccga
     4201 tttgaggcca tcatcaaagg taatcaccac cgctcgcgca gggagattga tcttattctt
     4261 cacgtaacct tccagctgca ccatgctcag tgtcgcgtat cccctgtcgc gcagccaggc
     4321 catctggtta ttgaaagcgc gtactgaggt ggtcgtcgaa gtatggcgaa aacgggtgtt
     4381 ttcttcatcg cgcagaatat ggtgataggt tagcaccggc aggccattat cgggttgggc
     4441 atccagcgcg ctgatatagg ccagtcgatc gccaatacgg atctgatacc aggtttgatt
     4501 taacctgtct ttcagtttat gcaaaatcgg gtagcgcaaa ttgtccgcca gtaccccata
     4561 atggcgcact gcccgcactc ggcgcgttat agaccggcgt atctttccag gtaattaagt
     4621 tctgattact cagcggcttg ttgaggtcac ccaaaccgtc ttcaactttt tgtcgcccct
     4681 gaactggctc gagataccct ttatcgataa accctttacc aaagccaaaa ttaaatgcgt
     4741 aataacttgc ggcagtgggc tccacggcaa tgatttgccc ggcccgaata ttccccacgg
     4801 tcaccatctt gtcgccaatt tgcgcccaga tcgcagcatt ttcgatggtt tgcatataac
     4861 gggcaggtaa cgcggcacta acgcttgcgg taaacagcat cagcaggatg aataacagct
     4921 tgtttataca tgaaaaaata accgagggtg aaacaacgct tgtattgtag caaaagcgcc
     4981 atcagaatcg cgggttaata ctcatacaaa ttatgtttga ggacaaaagg cggatttttt
     5041 tgttgttggt gccaaagact actcacctcc ggcgcaccca gttcgacaat acgctcgcga
     5101 aactctgaac tggtcatggt ttcacggcaa gccatcatct gttcaattaa cggtaacgtg
     5161 acaccagaaa tcacttcaca acgggagtgt ttatggctta ataatgaagc cacgcggtac
     5221 ggtggcgctc cggctatatc ggttaagaag atgacaccct caccagagtc cgcttcatgc
     5281 agagcatcgc acatcatgcg gctgagcata ttagagctta atccgcgcca gaaattcacg
     5341 gcccggcact gaagaagtgc cccatgtttt ttctccagcg catccagtat ctcttgcgcc
     5401 cggtcatcgt gacaggtaat tacccaacct aacatcgcct tcctccttag ccgaaaatca
     5461 gtctatcctg accattgttg ataaagggtg atagccgtca aaactcctct cccccgaatc
     5521 cagaggagag gaaatagcct tagctacgca aaccacgtcc acgttggatc agcgaccaac
     5581 agatcaaata aaacaccaca ataaagacca ccagtacgcc aaaggtagtg gccagcggca
     5641 catcatttat accgaggaag ccgtagcgga aaccactgat catataaacg attgggttca
     5701 ggtgcgacag cccttgccag aacggcggca acaaagtcag ggagtaaaac accccgccca
     5761 aatacgtgag tggcgttaac acaaaggttg gcaccaggct gatgtcatcg aacgttttgg
     5821 caaacacgcc gttcagcaaa cccgcaaggg agaatagcac cgccgtgagc accagggtta
     5881 aggcaacgaa tacccacgaa tgcacctgaa acggcacaaa aaacagtgaa attgccgtca
     5941 ccagaatgcc aacaaacaga ccacgcgcca cgccaccgcc gacatatccg gcaataatga
     6001 cgtgagtcgg aaccggcgct accagcagct cttcaatatt acgctggaac ttggcaccaa
     6061 aaaatgatga cgcgacgtta gcgtaggcat tggtgatcac cgacatcatg atcagccccg
     6121 gtacgatgaa ctgcatatag ctgaagccat gcatatcgcc aatacgcgaa cctatcaggt
     6181 taccgaagat aataaagtaa agggtcatgg tgatgactgg cggcaccagc gtctgcaccc
     6241 agatacgcat aaagcgatgg atctctttcg cccagatgct ttttagcgcc acccagtaaa
     6301 gatgcatcat gcgcgatctc cttgtttttc attaaccagt gaaacaaaca gctcttccag
     6361 acggttagct ttgttacgca tacttaatac ctgaatgccc tgctcactta actgcgtaaa
     6421 tacgctgttg atcccctgct cacgcagcac ttcaacttcc agcgtcgcgg tatcgaccag
     6481 tcgatactga tagccatcga gcttcggtaa cgggcttttc ggtgcgagat cgagaataaa
     6541 ggtttccaat ttcagcttcg ccagcagcgc cttcatcgag gtattttcca ccagctcacc
     6601 gtgttgaata atgccgatat tgcggcacag catttctgct tcttccaggt agtgtgtagt
     6661 gagaatgatg gtggtgcctt tgtcgtttaa atccttcaaa aagccccaca ttgagcggcg
     6721 aagttcaata tccacgcctg cggtcggttc gtcgagaatc agtagtttag gttcatgcat
     6781 taacgcacgg gcaatcatta aacggcgctt catcccgcca gataacatac gcgcacgttc
     6841 gttgcgtttt ccccataaat cgagttgttt gagatatttt tcgctgcgga tgtacgcttc
     6901 tttgcgctcc acgccgtagt accctgcctg attcaccaca atttgctgca cggtttcaaa
     6961 cgggttgaag ttaaattcct gcggcaccag tcccaactga cgtttagcgt tcacgacatc
     7021 cttctcgaga tcgtaaccaa atacgctgac ccgcccggag gttttattta ccagagagct
     7081 gataataccg atagtggtcg atttcccggc cccgttcggc ccgagaagcg cataaaaatc
     7141 acccgcttcg acctgcaaat ctattccacg aagcgcctga acgccgcctg gataggtttt
     7201 tttaagctgt tgaagttcca gtgcaatggt cataaatttt tacttacctt acgttcttac
     7261 actttatctg tggtttaaat cgtcccggag ttgccctata ttagccaaac gtaattattt
     7321 ggttacaggt cgttaacctc catgaaagac atagatacac tcatcagcaa caatgcacta
     7381 tggtcaaaaa tgctggtgga agaggatccc gggttttttg agaaactggc acaagcgcaa
     7441 aaaccgcact ttctatggat tggatgttcc gacagtcgcg ttcctgcaga acgtttaacc
     7501 ggtcttgagc cgggcgaact ctttgttcac cgtaatgttg ctaacctggt cattcacacc
     7561 gacctgaact gcctttccgt ggttcagtat gcagtggatg tactagaagt tgaacacatt
     7621 attatctgtg gccactacgg ttgcggcggc gtacaagccg cagttgaaaa cccggaactg
     7681 gggcttatca acaactggct gctgcatatc cgcgatatct ggttcaaaca tagctcattg
     7741 ctcggcgaaa tgccgcaaga gcgccgtctg gataccttgt gtgaactgaa cgtcatggaa
     7801 caggtgtata acctaggcca ctccaccatt atgcaatcag cgtggaaacg cgggcagaaa
     7861 gttaccattc acggctgggc ctatggcatt cacgacggct tgctgcgtga tctggatgtt
     7921 actgccacca accgcgaaac ccttgagcaa cgttaccgtc acgggatttc caacctcaag
     7981 ctgaaacacg ccaaccacaa ataaaaatgc catgccggat gcaacacatc cggcaacttc
     8041 acacttactc gtccagcaga atcactttgc cgatatacgg cagatgacgg taacgctgtg
     8101 cgtaatcaat gccgtaaccc accacaaact catccgggat cgagaaaccg ataaattcta
     8161 ccgggacgtt cacttcacga cgggacggtt tatccagcag cgtacaaatc gccagcgact
     8221 tcggttcgcg caggcttaag atctcacgca ctttcgacag tgtattcccc gagtcgatga
     8281 tatcttcaac aatcagcacg tccttgccac ggatatcttc atccagatct ttgaggattt
     8341 tcacatcacg ggtggtggac atgccgctac cgtagctgga ggcggtcata aagtcgactt
     8401 catgagatac ctgaacttca cggcacaggt ccgccataaa cataaatgag ccacgcagca
     8461 gacccaccag caccatatcg ctgccgctgt ctttgtaacg ctcagtaatc tgacgaccca
     8521 gttcggcgat acgcgcttta atctccgctt cggggatcat tacttctaca gtatgtttca
     8581 tatctctaac catatgattt aaaaacaaat cacttcgcac gggcaggttt tggcaccgca
     8641 acaaagcgca agacacgcag tataccagta aaactattca ttcgcggaat ctgttaataa
     8701 agctcgaatt gtgatgacga tcacacatgt taaaacctat aattaattgc tactaaaata
     8761 ttaatgaatt gaaatggtgt ctctttatgg caattaacaa tacaggctcg cgacgattac
     8821 tcgtcacgct aacagtcctt tttgcagcgc tttgcgggct gtatctactc attggcggag
     8881 gctggctggt cgcgattggc ggctcctggt actaccctat cgctggcctt gtgatgctcg
     8941 gcgtcgcctg gatgctgtgg cgcagtaaac gtgccgcgct ttggctatac gccgccctgc
     9001 tgctcggcac catgatttgg ggcgtctggg aagttggttt cgacttctgg gcgctgactc
     9061 cgcgcagcga tattctggtc ttcttcggca tctggctgat cctgccgttt gtctggcgtc
     9121 gcctggtcat tcctgccagc ggcgcagttg ccgcactggt ggtggcactg ctgattagcg
     9181 gtagtatcct gacctgggcc ggatttaacg atccgcagga aatcaacggc accttaagcg
     9241 ccgatgccac acctacggaa gctatctcac ccgttgccga tcaggactgg cctgcctatg
     9301 gtcgcaatca ggaaggtcaa cgcttttcac ctctgaaaca aattaacgcc gataacgtcc
     9361 ataatctgaa agaagcctgg gtgttccgta ctggcgatgt gaagcagccg aacgatccgg
     9421 gcgaaatcac caatgaagtg acgccgatta aagtgggcga caccctttac ctgtgtaccg
     9481 ctcaccagcg cctgtttgcg ctcgatgccg ccagcggcaa agagaaatgg cattacgatc
     9541 ctaagctgaa aactaacgag tctttccagc acgtaacctg ccgtggcgtc tcttatcatg
     9601 aagccaaagc agaaacggct tcgccggaag tgatggcgga ttgcccgcgt cgtatcattc
     9661 ttccggtcaa tgatggtcga ctgattgcga ttaacgctga aaacggcaaa ctgtgcgaaa
     9721 ccttcgccaa taaaggcgtg ctcaatctgc aaagcaatat gccagacacc aaaccgggtc
     9781 tgtatgaacc gacttcgcca ccgattatca ccgataaaac catcgtgatg gcaggttcag
     9841 ttaccgataa cttctcaacc cgcgaaacgt ctggcgtgat ccgtggtttt gatgtcaaca
     9901 ccggggagct gctgtgggct tttgatcccg gcgcgaaaga tccgaacgca atcccgtctg
     9961 acgaacacac ctttaccttt aactcgccaa actcttgggc accagcggcc tatgacgcga
    10021 agctggatct ggtctatctg ccgatgggcg tgaccacgcc ggatatctgg ggcggtaacc
    10081 gcacaccgga acaggaacgt tatgccagct cgattctggc gctgaatgcc actaccggga
    10141 aactggcgtg gagctaccag accgttcacc acgacctgtg ggacatggat cttccggcac
    10201 agccgacgct ggcggacatc accgttaatg gtcagaaagt gccagttatt tacgctccgg
    10261 cgaaaaccgg caacattttt gtgctcgatc gtcgcaatgg cgaactggtg gttccggccc
    10321 cggaaaaacc ggtgccgcaa ggtgctgcga aaggcgatta cgttactccg acccagccgt
    10381 tctctgaact gagcttccgt ccgaagaaag atttgagcgg tgcggatatg tggggagcca
    10441 ccatgtttga ccaactggtg tgccgcgtga tgttccacca gatgcgctat gaaggcattt
    10501 tcacgccgcc atctgaacag ggcacgctgg tcttcccggg taacctggga atgtttgaat
    10561 ggggcgggat ttccgttgat ccaaatcgtg aagtggcaat tgccaaccca atggcactgc
    10621 cgtttgtttc gaaactgatc ccacgcggtc cgggtaaccc gatggagcag ccgaaagatg
    10681 ccaaaggcac gggtacggaa tccggcattc agccacagta cggtgtaccg tatggtgtca
    10741 cgctcaaccc gttcctctca ccgtttggtc tgccgtgtaa acagccagca tggggttata
    10801 tctcggcgct ggatctgaaa accaatgaag tggtgtggaa gaaacgtatt ggtacgccgc
    10861 aggacagtat gccgttcccg atgcctgtac cggtgccgtt caatatgggt atgccgatgc
    10921 tgggcgggcc aatctccacg gcgggtaacg tgctgtttat cgccgctacg gcagataact
    10981 acctgcgcgc ttacaacatg agcaacggtg aaaaactgtg gcagggtcgt ttaccagcgg
    11041 gtggtcaggc gacgccaatg acctatgaag tgaatggtaa gcagtatgtg gtgatctccg
    11101 caggcggtca cggttcattt ggtacgaaga tgggcgacta tattgtggct tatgccctgc
    11161 cggatgatgt gaaataacca taatgcccgg agagatccgg gcatatttcc gaatacggtc
    11221 tttttatacc gtaaacccta acatcatccc cgtatcttca tgctccagca gatggcagtg
    11281 cgccatataa gcatgttctt tcggtgcgtc gtgattaaac ttcaccagca cttcactgac
    11341 attgccttct accttaacgg tatctttcca gcccgcgcga tgagccgctg gcggtttgcc
    11401 attttctgac aagatacgga actgcgtgcc gtgaatatgg aacggatgca gcatcatgtc
    11461 gcccacgcca gagataaccc aacgttcgta ctgccctttc gccgccgcaa acatcggctt
    11521 attcatatca aacgcctgac cgttgatttt attggcatgg tggaaatcga acttcccgcc
    11581 gtggttcata tgattcatat taccgtgccc catatggccc atcatctggc tgtgatccat
    11641 tccggccatc gcctgatcgc catatttctc cattagcatc tgcataccca tcatatcgag
    11701 catcgggtcc atagaaagtt gcagcttgcg taccgtcagc ccttccagcg aaggtaacgc
    11761 aggcaggcta cttaatgtgt ctggcaaagc accggaggca ctaatagcaa tcggctgaat
    11821 ccgcattacc ggatgcggct tgtcaaacgg cgcaatcgcc atccccatct ggctgaccgg
    11881 tagcgtcacc aggtcaaagg gtttgttgtc gttaacctcc accagcactt caaaacgctc
    11941 gcccatcagc accggcagct cgctcacctt caccggttca ggtagcagac caccgtcgct
    12001 ggcaatcaca tacagcgggc gattgtcgct ggtggcgaaa ttgagcgagc gggcattaca
    12061 gccattgagc aaacgcaggc gcagccaacc acgcggggca gcgtgttgtg ggtagattgc
    12121 accgttggtc agcagcgtat cgccaaacca gcccacggcg gcggtcatca catccagttg
    12181 ataatcaatc tgcccatcag cgttaaattt tttatcctga acaatcaccg gaacatcatc
    12241 gataccccac tgttttggca gcattaattt caggatctcg tcatcttcaa ttaccaccag
    12301 cccagccaac cccatcgcca cctgtcgccc agtcttgcca tgttgatgtg gatggaacca
    12361 gcaagtagcg gcaggttgat caacgttcaa cgtcaccgag cgcttgccac ctggcggaat
    12421 aattccctgc gggccgccgt cgacttcacc cggtacttcc agcccgtgcc agtgcaacgt
    12481 cgtctcttcc gtcagttggt tatagatatc aaccgttacc gctttgccgc gctgtaattt
    12541 caccgccggc cccagcagat tgccgttata gccccaggta gttgcagttt tctcgccaaa
    12601 ggtagactga cctgcgccaa tagttaactg aatgcgatta cgggcatcgg tcgtgagcaa
    12661 atcagggatc ggtaacgttg ggcgttccgc cgcaaagact gcgcggctcc acagcggcaa
    12721 ggctgaagcc acacccagcg cgacggaata ttttaagaaa tcacgacgtt gcatagttat
    12781 ttccttattc ttaagcaggc gaaacaaaat caaacgttga gcatagtcct tccccttacg
    12841 ggaaggtcaa gccgcagaca caatttaatc gacaataatg gtcgtcgcct cgctcgcagt
    12901 gtgctaacgt ttatcttctt taagcccctg gtagaagcaa tgaagacgtt tttcagaaca
    12961 gtgttattcg gcagcctgat ggccgtttgc gcaaacagtt acgcgctcag cgagtctgaa
    13021 gccgaagata tggccgattt aacggcagtt tttgtctttc tgaagaacga ttgtggttac
    13081 cagaacttac ctaacgggca aattcgtcgc gcactggtct ttttcgccca gcaaaaccag
    13141 tgggatctca gtaattacga caccttcgac atgaaagccc tcggtgaaga cagctaccgc
    13201 gatctcagcg gcattggcat tcccgtcgct aaaaagtgca aagccttggc ccgcgattcc
    13261 ttaagcctgc ttgcctacgt caaataatcc ctgatacctt tttgtagaaa tattgaccgt
    13321 gcatccgcgg tcaatgttag ctattatgtt gcgccctttt tttacgggtg ttaacaaagg
    13381 aggtatcaac ccatggccga aaaaaaacag tggcatgaaa cgctacacga ccagtttggg
    13441 cagtactttg cggtagataa cgttctgtat catgaaaaga ccgatcacca agatctgatc
    13501 atttttgaga acgctgcatt tggtcgcgta atggcgctgg atggcgtagt acaaaccacc
    13561 gagcgcgacg agtttatcta tcatgagatg atgacccatg ttccgctact ggcccacggt
    13621 cacgcgaaac atgtgctgat tatcggcggc ggcgacggtg ccatgctgcg tgaagtaacc
    13681 cgacataaaa acgttgagtc aatcacgatg gtggaaatcg atgcgggtgt cgtgtcgttc
    13741 tgccgtcagt atctgcccaa ccataacgcc ggtagctacg acgatccgcg ctttaagctg
    13801 gtgatcgacg atggcgtcaa tttcgttaat caaaccagcc agacctttga tgtcattatc
    13861 tccgactgca ccgatcctat cggtcccggc gaaagtctgt tcacctcagc attttatgaa
    13921 ggctgcaaac gttgcctgaa tcctagcggt atcttcgtcg cacaaaacgg cgtctgcttt
    13981 ttacagcagg aagaagccat cgacagccat cgcaaactca gccattactt cagcgacgtt
    14041 ggcttttatc aggcggcgat cccgacctat tacggcggta tcatgacttt tgcatgggcg
    14101 acagataacg acgccttacg ccatctctca accgaaatta ttcaggcgcg ttttctcgct
    14161 tctggcctga aatgccgtta ttacaatccg gcaatccata cggcagcttt tgccttacct
    14221 cagtatctgc aagatgcact ggcttcacag ccgtcctaag gagaagataa gaaattgaaa
    14281 aaactgaaac tgcatggctt taataatctg accaaaagtc tgagtttttg tatttacgat
    14341 atctgctacg ccaaaactgc cgaagagcgc gacggttata ttgcttatat cgatgaactc
    14401 tataatgcca accgtctgac cgaaatcctg tcagaaacct gttccattat cggggccaat
    14461 attcttaaca tcgcccgcca ggattacgaa ccacagggtg ccagcgtcac tattctggtg
    14521 agtgaagaac cggttgaccc gaaactcatc gacaaaacag aacaccccgg cccactgcca
    14581 gaaacggtcg ttgcccatct cgataaaagt catatttgcg tacataccta cccggaaagc
    14641 catcctgaag gtggtttatg taccttccgc gccgatattg aagtctctac ctgcggcgtg
    14701 atttctccgc tgaaggcgct gaattacctg atccaccagc ttgagtccga tatcgtaacc
    14761 attgattatc gcgtgcgcgg ttttacccgc gacattaacg gtatgaagca ctttatcgac
    14821 catgagatta attcgattca gaactttatg tctgacgata tgaaggcgct gtatgacatg
    14881 gtggatgtaa acgtctatca ggaaaatatc ttccatacca agatgttgct taaagagttc
    14941 gaccttaagc actacatgtt ccacaccaaa ccggaagatt taaccgacag cgagcgccag
    15001 gaaattaccg ctgcgctgtg gaaagaaatg cgcgagattt attacgggcg caatatgcca
    15061 gctgtttaac ggctctggcg gagctccagg ctccgccaga tttatttact tctgctgcac
    15121 gaaattgcgg taagccgcca cgacctgcag aaaatcctca acgccgcata gcgacaggct
    15181 ttcttcgtcg tagtagttca tcccctcttc catttcatcg ccagcgaatt ccagttgatt
    15241 ggcgcgaacc atcacctctt caccgtccat ccacagggtg tattcatgcc ctgcccgttg
    15301 ccaggaccgt tcgctacctt tcagtgcgtg cgctgcttgt tccacttcat caagcaaggc
    15361 caggttttct ttcacctctt cattaaacca gtgcccgacc acttcatgcc ccatggacat
    15421 acgcaccttt accactccgg taatatcgcg cagaaattcg taatccatag tgtgttcctc
    15481 tgctcccggc aatgcgccga aatcgcattg cgccattgat gtaattatcg cagccttacc
    15541 ggagaaaaac agcggaacgg cgatggttta gaaataaaaa atgcccggga atctcattca
    15601 ccgggcattg tgtcgtttat gcgcagcgcg tgcgcttact tattacaccg cagtctggaa
    15661 aatcacccca tcggcttttt cggtgtactg agaaagttgg ttgaagttca gataacgata
    15721 agtatcaacg gcagttttat ctacttgcgc aacgtaggtc tggtactctt ccggcgtcgg
    15781 cagtttgcca atcagcgccg caacagccgc cagttctgca gaagccagga agacattcgc
    15841 gccagtcccc agacggttcg ggaagttacg ggtagaggtg gaaaccaccg tcgcaccgtc
    15901 tgctacacgc gcctggttac ccatacacag agaacagccc gggatctcga tacgagcccc
    15961 gctcttaccg aagacgctgt agtagccttc ttcagtcaac tgtgcggcgt ccatacgggt
    16021 tggcggtgcc acccacaggc gggtcggcaa ctggccttta tgcgcatcca gcagtttacc
    16081 cgcagcacgg aagtgaccga tgttagtcat gcaagaaccg ataaatactt cgtcgatctt
    16141 ctcgccctgt accgcagaca gcggacgcgc gtcatccggg tcgttcggag cacacaggat
    16201 tggctcttta atatccgcca gatcgatgtc aatcactgcc gcgtattccg catctgcatc
    16261 ggcttccagc agctcaggat tcgccagcca tttttccatg ccctgaatac gacgttccag
    16321 ggtacgacga tcgccgtaac cttccgcgat catccacttc agcaggacga tattagagtt
    16381 aaggtattcg atgattggtt ctttgttcag cttgatagta caaccagcgg cagaacgctc
    16441 agcggacgca tcggtaagct caaaggcctg ctcaactttc agatccggca gaccttcaat
    16501 ttccagaatg cggccagaga agatgttttt cttgcctttc ttctcaacgg tcagcagacc
    16561 ttgtttgatg gcatacagcg gaatggcgtg taccagatcg cgcagggtga tgcccggctg
    16621 cattttgcct ttgaagcgca ccagaacgga ttccggcata tccagcggca ttacgcctgt
    16681 tgcggctgca aatgccacca gaccagaacc cgccgggaaa gagataccga tcgggaaacg
    16741 ggtatgggag tcacctccgg taccgacggt atccggcagc agcatacggt tcagccagga
    16801 gtggattacg ccgtcacccg gacgcagcga cacaccgcca cggttcataa tgaagtccgg
    16861 cagcgtgtgg tgcgtgttca cgtcaactgg cttcggatac gccgcggtgt ggcagaaaga
    16921 ctgcatcacc aggtcagccg agaagcccag gcacgccagg tctttcagtt catcacgggt
    16981 catcgggccg gtagtgtcct gtgaacctac agaagtcatt ttcggttcgc agtacgcgcc
    17041 cggacgaatg cctttcacgc cacaggcacg gcctaccatt ttctgcgcca gcgagaagcc
    17101 gcgatcgctc tcagcgacat ctttcgcctg acggaacaca tcactgtgcg gcagaccaag
    17161 tgcttcacgc gctttggtgg tcaggccacg cccgataatc agcgggatac ggccgccagc
    17221 acgcacttca tcaatcagca cgtcggtttt cagttcgaag gtcgccagca gttcgcctgt
    17281 ttcgtggtta cgcacttcac ctttgtacgg gtaaacgtca atcacgtcgc ccatgttcag
    17341 gttagagacg tcgacttcga ttggcagtgc acccgcgtct tccatcgtgt taaagaagat
    17401 gggtgcaatt ttaccgccga ggcacaaacc accgccgcgt ttgttcggca catgtggaat
    17461 atcatcgccc ataaaccaca gaacggagtt cgtggcggat ttacgcgatg aacccgtacc
    17521 cacaacgtca ccgacgtacg ccagcgggaa acctttctgt tgcagagctt caatctgttt
    17581 gatcgggcca acaacacctg gctggtctgg ctcaatacct tcacgggcgt ttttcagcat
    17641 cgccagcgcg tgcagtggga tatccgggcg cgaccacgca tccggtgccg gagaaaggtc
    17701 atcggtgttg gtttcgccag tgactttgaa gacggtaacg gtcagttttt cagccagcgc
    17761 cgggcgattc ataaaccatt cggcatccgc ccaggactgc attacctgct tcgcatattc
    17821 gttgcctgct ttcgctttct cttctacgtc atagaagtta tcgaacatca gcagtgtgtg
    17881 agaaagtgct ttggcagcga tcggtgccag tttggcatca tccagcgcgt cgatcagcgg
    17941 atgaatgttg taaccaccct gcatggtgcc cagcagttcg atggcttttt ccggagtcag
    18001 cagaggggat ttggcttcgc ctttcgcgat agcagccagt aagcctgctt tgacataggc
    18061 ggcttcatcg acgccagggg gaacacggtt ggttaacaga tctaacagga attcttcttc
    18121 gcccgcgggc gggtttttca gcagctctac aagtgcggcc atttggtttg catccagggg
    18181 tttgggcgca atcccctcag cggcacgctc agctacgtgc ttacggtatt cttctagcac
    18241 gacggttctc ctcgctctca ttgtcatagt gcggcaggcg attctcttca cgctcctgtg
    18301 agacagcagt ttgtagggta aatgcccagt accgcaacgg gcagaatagc aggattttga
    18361 cgacgtgtta atctgtttac aaaaaagcaa cataaaaaag tggctgaatc gttaaggatg
    18421 gtctagagat tgtttccctc cgtaaaaact tcgttaattc gcatggtgat aatcaccttt
    18481 ctcaacgaat cccaagatta gtcaaaattt aaactaccgc ctctttatac tcggattaac
    18541 agcacctgcg ggtgacagtt cgccgaccat tgcgatttcc ttgagatccg aattatgaaa
    18601 atgactttgc cgtttaaacc ccatgtgctg gcactaattt gcagtgccgg gctttgtgcc
    18661 gcctctgccg ggctatatat aaaaagccgc acagtggaag cggctgtgga aacgcaatcg
    18721 acacaactgg ctgtgtctga cgctgccgcg gttacgcttc ctgcaacggt ttccgcgcct
    18781 cccgtaacac ccgccgtcgt taaatccgca ttcagcactg cacaaataga tcaatgggtc
    18841 gcgcccgtcg cgctgtatcc cgacgcccta ctttcgcagg tgctgatggc atcaacctat
    18901 ccggcaaacg ttgctcaagc agtgcaatgg tcgcacgata atccacttaa acaaggcgat
    18961 gctgctattc aggcggtatc tgaccagccg tgggacgcca gcgttaaatc actggtggcc
    19021 tttccacaat tgatggcatt gatgggcgaa aacccgcaat gggtgcaaaa cctgggcgat
    19081 gcttttctgg cgcagccgca ggacgtgatg gactcggtac agcgattgcg gcaactggcg
    19141 caacaaaccg gttcgctgaa gtcatcaaac gaacaaaaag ttattaccac aacgaagaaa
    19201 gctgtaccgg taaaacagac agtcacggca cccgtcatac catccaatac cgttttaact
    19261 gccagccccg tcattacaga gcctgcaaca accgtcattt ccattgagcc cgccaatcct
    19321 gatgtggtct atattcccaa ctacaaccca accgtggttt acgggaactg ggccaatact
    19381 gcgtatccgc cggtttatct gccaccacca gccggagaac cgtttattga cagctttgtg
    19441 cgcggattcg gctatagcat gggtgttgct accacgtacg cactattcag cagcatcgac
    19501 tgggatgacg acgatcatga ccatcatcat catgacgatg atgattatca tcaccacgat
    19561 ggcggtcatc gtgacggtaa tggctggcaa cacaacggcg acaacatcaa tatcgacgtc
    19621 aacaatttca accgtatcac cggtgagcat cttactgata agaatatggc atggcggcac
    19681 aatccaaact accgtaatgg tgtgccctat catgatcagg atatggcaaa gcggtttcat
    19741 caaaccgatg tcaacggcgg aatgagtgcc acgcagctac ctgctccaac acgcgacagc
    19801 cagcgtcagg cggcagcaag tcagtttcag caacgaacac acgccgcccc cgtcattaca
    19861 cgagataccc aacgtcaggc agcggcacag cggtttaatg aagctgaaca ctatgggagc
    19921 tatgacgact tccgcgactt cagccgtcgc caacccctga cccagcaaca aaaggacgcc
    19981 gctcgtcagc gttatcagtc agcttctcct gagcagcgcc aggcagttcg cgagagaatg
    20041 cagactaacc cgcagatcca gcagcgaaga gaggcagcgc gtgagcgcat tcagcccgcc
    20101 tcgcctgagc agcgccaggc agtccgcgag aaaatgcaga ctaacccaca gatccagcag
    20161 cgaagagacg cagcgcgtga gcgtattcag tcagcctcgc ctgagcagcg ccaggtgttt
    20221 aaggaaaaag tacagcagcg cccactgaac caacagcaac gtgataacgc ccgccagcgt
    20281 gttcaatcag catcacctga acaacgtcag gtttttcggg agagagttca ggagagccgc
    20341 ccacaacgtc taaacgacag taaccgtact gccagattga ataacgatca acggtcagca
    20401 gtacgcgaac gtctctctga gcgcggagca aggcgactgg aaaggtaaat tacagacgta
    20461 aaaaaagcgg cgtggttagc cgctttttta attgccggat gttccggcaa acgaaaaatt
    20521 acttcttctt cgctttcggg ttcggcaggt cggtaatgct accttcgaac acttctgccg
    20581 ccaggcccac agactcgtgc agagtcgggt gcgcatggat ggtcagcgcg atgtcttcag
    20641 cgtcacaacc catttcgatt gccaggccga tttcacccag cagctcgccg ccgttggtac
    20701 cgacaatcgc accaccgatc acacggtgag attctttgtc gaaaatcagc ttggtcatac
    20761 cgtctgcgca gtcggaagcg atagcacgac cagaagcagc ccacgggaag gtggcggttt
    20821 catagctgat gcctttctct ttcgcttctt tctcagtcag acctacccat gcaacttctg
    20881 gttcggtata ggcgatagac gggataactt tcggatcgaa gtagtgtttt ttaccggcga
    20941 taacttcagc ggcaacgtga ccttcgtgaa cacctttgtg tgccagcatc ggctgaccaa
    21001 cgatatcgcc gatagcaaag atgtgcggta cgttggtacg cagctgtttg tcaacgcgga
    21061 tgaaaccacg gtcgtccact tccacgccag ctttgcctgc gtcgaggttt ttaccgttcg
    21121 gcacacgacc aatcgctacc agcacggcgt cgtaacgctg cggttcagcg ggtgcttttt
    21181 tgccttccat cgtcacataa ataccgtctt ctttcgcttc aacggcggta actttggttt
    21241 ccagcatcag gttgaatttc ttgctgatac gcttggtgaa gactttaacg atgtctttgt
    21301 cagctgccgg gataacctgg tcgaacattt caaccacgtc aatctgtgaa cccagcgcgt
    21361 ggtatacggt gcccatttcc agaccgatga taccgccacc cattaccagc aggcgttctg
    21421 gtacttcttt cagttccagc gcgtcagtgg agtcccagat acgcggatct tcatgcggaa
    21481 taaacggcag ttggatcggg cgagaacccg ctgcaatgat cgcgttgtcg aagttgatca
    21541 cggttttacc gttctcacct tcaacttcca gggtgttagc cccggtaaat ttacccagac
    21601 cgttgaccac tttgactttg cggcctttcg ccataccagc cagaccaccg gtcagctgat
    21661 tgatcacttt ctctttccag gtacgaatct tgtcaatatc ggttttcggt tcgccgaaga
    21721 cgataccgtg ttcagccagc gctttggctt cttcgataac ttttgctacg tgcagcagtg
    21781 ctttagaagg gatacagccg acgttcaggc aaacaccgcc aagggtgttg taacgttcta
    21841 cgattacggt ttccagacct aaatcagcgc aacggaaggc agcggagtaa cctgcggggc
    21901 ctgccccaag taccacgacc tgagttttga tttcagtact catcatgacc tctatatatt
    21961 tatctccggc ggtcataccc gtcgtctttc aggccgcagc ggcgttagct tcacgccctc
    22021 accccagtca cttacttatg taagcccctg gggattctgg cgcttgctac cttgctgcac
    22081 ccagaaatcc atagggtatg tgaagtaata ccctaaccac caccgggtcg ttctatccgt
    22141 cggtatttta caaaattgtt aacaattttt aaacaacaaa cggcaaccga tttgtctatt
    22201 cgctaataac ctcaatacat tcatgagatt accagaaaaa agccggccgt tgggccggct
    22261 cttttactta catcaccaga cggcgaatgt cagacagcgt gttgttaatg atggtaatga
    22321 aacgggcacc atcagcaccg tcgatcacgc ggtggtcgaa ggagagagaa atcggcagca
    22381 tcagacgcgg cacgaactct ttaccattcc acaccggctc catcgcggac ttggaaacgc
    22441 cgaggatagc cacttccggc gcgttcacaa tcggcgcgaa gtgggtagta cccaggccgc
    22501 cgatgctgga gatggtgaag caaccgccct gcatttcgcc cgcagtcagc ttaccgtcac
    22561 gcgctttctt agaaatagtc atcagctcgc gagacagctc gatgatgcct ttcttgttga
    22621 cgtctttgaa taccggaaca accagaccgt tcggggtatc caccgccaca ccgatgttga
    22681 tgtatttctt cagggtcaga cgctgaccgt cttccgacag cgaactattg aagcgaggca
    22741 tctgctcaag agctgcagca acggctttca tgatgaagac aaccggggtg atcttcacat
    22801 ccagcttacg tttcgccgct tcttcgttct gctgtttacg gaacgcttcc agctcggtga
    22861 tatcggtttt gtcgaagtgg gtaacatgcg ggatcatcac ccagttacgg ctcaggttag
    22921 caccagaaat tttctggata cggcccagtt ccacttcttc gatttcacca aacttgctga
    22981 agtccacctt cggccacggc agcatgcctg ggataccgcc gccagtcgcc gccggagctg
    23041 cttctgcacg tttgatagct tctttcacgt aagcctgaac gtcttcgcgc aggatacgac
    23101 ctttacggcc agtgcccttc actttcgcca ggttaacacc aaactcgcgt gccagacggc
    23161 ggatcagcgg agtcgcgtga acataagcgt cgttttcagc aaattcagat ttgccttccg
    23221 ctttcgcagc tggtgctgct gccggggctt cagcttttgc tgccggtgcc ggcgctgccg
    23281 cttcctgttt cgcaggagct gccgcaggcg ctgcgccttc aacttcgaag atcataatca
    23341 gcgagccagt tttcacttta tcgccaacgt tgactttcag ttccttcacg acgcctgcaa
    23401 acggcgccgg aacttccata gaagctttgt cgccttctac ggtgatcagt gactgttcag
    23461 cggcaacttt gtcgcccact ttcaccatca cttcagtcac ttcaacttcg tcaccgccga
    23521 tatccggaac gttaacttct ttcacgccag ctgctggtgc aggggccgct gccggagccg
    23581 cttcctgttt agccgccgga gctgccgcgc ctgcttcacc cgcgacttcg aagaccataa
    23641 tcagcgagcc ggtagacact ttgtcaccca cgttcacttt gatctctttc acggtgccag
    23701 caaacggagc cggaacttcc atagaagctt tatcgccttc tacggtgatc agcgactgtt
    23761 cagcttcaac tttatcgcca actttcacca ggatttcggt cacttcaact tcgtcgctgc
    23821 cgatatccgg aacgttaacg tcttttgccg ccgcagccgc gggtgctgct gccggagctg
    23881 cttctttctt ctcttctgcc tgagcaggtg cagcgtctgc tgcaccgtcg gcggaatcga
    23941 aaatcataat cagtgcgccg gtctgggttt tatcgccaac agagactttg atctctttaa
    24001 cgatacccgc ctgcggagac ggaacttcca tagaggcttt gtcgccttct acggtgatca
    24061 gcgactgttc ggcttcaact ttgtcgccca ctttgaccag gatctcggtg atttcaactt
    24121 catcagcccc gatgtccggt actttgattt cgatagccat tattctttta cctcttacgc
    24181 cagacgcggg ttaactttat ctgcatcgat gttgaatttg gcgattgcgt cagcaaccac
    24241 tttcttatcg atttcgccac gtttagccag ttcgcccagc gccgcaacca cgacataaga
    24301 agcatcaact tcgaagtggt gacgcaggtt ctcacggctg tcggaacgac cgaagccatc
    24361 agtacccagt acgcggtagt cgtcagccgg tacgtaagta cggacctgct cagcgaacag
    24421 tttcatatag tcggtagatg ccactgccgg agcgtcgttc atcacctgag cgatatacgg
    24481 aacgcgcgga gtttccagcg gatgaagcat gttccagcgt tcacaatcct gaccatcacg
    24541 cgccagctcg gtgaaggagg tcacgctata aacgtcagaa cctacgccgt aatctttcgc
    24601 caggatctct gccgcttcac ggacgtgacg caggatagaa ccggagccca gcagctgaac
    24661 tttacctttg ctaccttcaa tggtttcgag tttgtagata cctttacgga taccttcctc
    24721 agcaccttcc ggcattgccg gcatgtggta gttttcgttc agcgtggtga tgtagtagta
    24781 aacgttctct tgtttttcac cgtacatacg ctccagaccg tcatgcataa tgacagcaac
    24841 ttcgtaagcg taagccgggt cgtaagagat acagttcgga atagtcagcg actgaatgtg
    24901 gctgtgacca tcttcatgct gcagaccttc tccgttcagg gtggtacgac cggaagtacc
    24961 gccgatcagg aagccacgcg cttgctggtc gccagccgcc cagcacagat cgccaatacg
    25021 ctggaagccg aacatcgagt aatagatgta gaacgggatc atcggcagat tgttggtgct
    25081 gtaagaggtc gccgctgcca gccaggaaca acctgcgccc agctcgttga tcccttcctg
    25141 cagaatctga cctttctcgt cttctttata gtaagcaacc tgctcgcggt cctgcggggt
    25201 gtactgctga ccgttcgggc tgtaaatacc aatctgacgg aacagacctt ccataccgaa
    25261 agtacgcgct tcgtcggcga tgatcggtac cagacgatct ttgatcgact tgttcttcag
    25321 cattacgttc agggcacgaa cgaaagcgat agtggtagag atctctttgc tctgctcttc
    25381 cagcagcgcg ccgaagtctt gcaggctcgg cagctcaagc ttctcggtga agttcggctg
    25441 acggcttggc agataaccgt gcagtttctg acgctgtgcg tgcagatagg tatgctcttc
    25501 agaaccttcc gggaaggtga tgtacggcag tttttcgata tctgcatcag acaccggcac
    25561 attgaaacgg tcgcggatgt gacgcacgcc gtccatgttc attttcttaa cctggtgcgc
    25621 gatgttttta ccttcagccg cgtcgcccat gccgtaacct ttaatggaat gagcaaggat
    25681 tactgtcgct ttgcctttgg tttcctgcgc tttcttgaat gcagcgtaga ttttcttcgg
    25741 atcgtgacca ccacggttca gtgcccagat ctgctcgtca gtccagtctg caaccagtgc
    25801 tgcggtttca ggatatttac cgaaaaagtg ttcacgaacg tacgcaccat ctttcgattt
    25861 gaaggtctgg tagtcgccgt caacggtttc gttcatcagc tggatcagtt taccgctggt
    25921 atctttacgc agcagttcat cccaacggct accccacatc actttgatca cgttccagcc
    25981 agcaccttcg aagatgcctt ccagttcgtt gatgatcttg ccgttaccgg tgaccgggcc
    26041 gtcaagacgc tgcaggttac agttgataac gaagaccagg ttatccagtt tttcacgggt
    26101 agcgatggtg atcgcacctt tggattccgg ttcgtccatt tcaccgtcgc cgaggaacgc
    26161 gtaaacggtc tgtttagagg tatctttcag gccacggtgt tccagatatt tcaggaattt
    26221 agcctggtaa atagcaccaa tcggccccag ccccatagat acggtcggga actgccagaa
    26281 ttccggcatc agtttcgggt gcggatagga agagaggcca ttgccgtgaa cttcctgacg
    26341 gaagttatcc agctgctcct gagtcagacg accttccagg aaagcacgag cgtacacgcc
    26401 cggggagatg tggccctgga agtaaaccag gtcgccgcca tcctgctcgt tgcgtgcacg
    26461 gaagaagtgg ttaaagcaca catcataaat ggttgcggaa gactggaagg acgccatatg
    26521 gccgcccagt tcgaggtctt ttttcgacgc acgcagcacc gtcatgatgg cgttccagcg
    26581 gatagctgaa cgaatacggc gttccagttc cagattaccc ggatactccg gttgttcttc
    26641 aacggggatg gtgttgatgt agttgctgat acctgtgcct gcggctacgt ttacaccgcc
    26701 tttgcgggct tcagcaagca gttggtcgat cagatactga gcacgctcaa caccttcttc
    26761 acggatgacc gattcgatcg cctggagcca gtcgcgagtt tcgatcggat ccacgtcatt
    26821 tgggaaacgt tctgacatgg gttattcctt atctatctaa taacgttgag ttttctggaa
    26881 cctgtcccat tgaactctcg ccggaaagct caataagaca ggttctacgt ttagttgccg
    26941 cgcttttata tgcgcttgat ttacaacatc ttctggataa tttttaccag taaaatcact
    27001 aattctttcg ttgctccaga cgacgcagag aacgctcacg gcggctctct tcacgacttc
    27061 tgtcgagcaa aatttcttcg ataaaggcca gatggcgatg cgatgcttcg cgcgcttctt
    27121 ccggcttacc ggccataatc gcttcaaata tgcgggtgcg gtgactactc accagcggca
    27181 gcatctcgcg acgcgaatag agcaattcga agttctggcg gacattctgg gccaacatcg
    27241 gctccataca ccttagcaga tgaagcagaa ccacattgtg ggccgcttca gtgacagcaa
    27301 tctgatactg gagtacggcg tttgattccg cgtccagatc gccagactgc tgcgccagtt
    27361 ctatggcgtg atggagttca cggatgcgtt ccttgtcttc atcggtacta cgcagcgcgg
    27421 cgtaataagc ggcgatacct tccagggcgt gtcgtgtttc gagcaagtca tactgtgact
    27481 caggatggtc ggagagcagc tccaccagcg gatcgctgaa gctttgccat aggctgctct
    27541 ggacaaaagt gccgccaccc tggcgacgaa gcaacaagcc cttcgcttcg agacgttgaa
    27601 tcgcctcacg caaggaggga cgggagacgt caaactgttt tgccagttcg cgttccggtg
    27661 ggagtttttc gcccgggcgg agagtgcctt cgaggatcaa aaactccagt tgctgctcaa
    27721 tcacatcgga gagttttggt tggcggattt tgctgtaggc catgagttcc tgtcttaagc
    27781 cacttgccga agtcaattgg tcttaccaat ttcatgtctg tgacgctaaa gtaacaaagt
    27841 attcacctta tgtccataca ggttttgatt gaaatcatga aactgtgcac attttaacaa
    27901 cttgacatat ataacgtttc aaagttgtaa ctatgcacaa atgtagactt tacgtaggaa
    27961 agggattttt taacgattat taaactataa aaatccgaat tgaaccgatt cacttaccaa
    28021 ttttgtgatt tttaattcaa ttaaaacgaa tttaaattca ttctacatat tgagaggggt
    28081 tgaggctgag ctttacaaac ggtttctttt taagcaactc atcttcaacc atgcataaag
    28141 cgggtgcatt cgctgccgca taccattatt cttgatctga cggaagtctt tttgtaacaa
    28201 ttcaaacttc tttgatgtaa acaaattaat acaacaaacg gaattgcaaa cttacacacg
    28261 catcactgcg tagatcaaaa aaacaaccac cgcacgaggt ttcatgatgg aaggtcaaca
    28321 gcacggcgag cagctaaagc gcggccttaa aaaccgccat attcagctta tcgcgctggg
    28381 tggcgcgata gggaccgggt tattcctggg tagcgcctcc gtaatacagt ccgcagggcc
    28441 agggattatc ctgggttacg ccattgctgg ttttatcgcc tttctgatca tgcgtcagct
    28501 gggtgaaatg gtggtcgaag aacctgtcgc aggctccttt agccactttg cttataaata
    28561 ctggggcagt tttgccggtt tcgcctctgg ctggaactac tgggtactgt acgttttagt
    28621 tgccatggct gagctgactg ccgtgggtaa atacattcag ttctggtatc cggaaatccc
    28681 cacctgggtt tctgccgccg tattctttgt ggtgattaac gccatcaacc tgaccaacgt
    28741 taaagtgttt ggcgagatgg agttctggtt tgccattatc aaagttatcg ctgtggtagc
    28801 gatgatcatc ttcggcggct ggctgctatt cagtggcaac agcggcccgc aggcgaccgt
    28861 tagcaacctg tgggatcagg gtggtttcct gccgcacggc ttcaccgggc tggtgatgat
    28921 gatggcgatt atcatgttct cgttcggtgg tctggaactg gtggggatca ccgcagcaga
    28981 agctgataac ccggagcaaa gtataccgaa agcaactaac caggttatct accgcatcct
    29041 gattttctat attggttcgt tagccgttct gctctcactg atgccgtgga cccgcgttac
    29101 cgccgatacc agtccgtttg tgctgatctt ccacgagtta ggcgatacct ttgtggcgaa
    29161 tgcgctgaac atcgtggtac tgactgcggc gctctccgtg tacaacagct gcgtatattg
    29221 caacagccgt atgctgtttg gtctggcaca acagggtaat gcgccaaaag cgctggcgtc
    29281 tgtcgataaa cgtggtgtac cagtaaatac cattctggtg tctgcactgg taacggcgtt
    29341 gtgcgtactg attaactacc ttgccccaga gtccgctttc ggactgttaa tggcgctggt
    29401 ggtatctgca ctggtaatca actgggcgat gattagcctg gcgcatatga aattccgtcg
    29461 cgccaagcag gaacaaggcg tggtaactcg cttccctgct ctgctttatc cgctgggtaa
    29521 ctggatctgc ctgctgttta tggcggcggt actggtgatt atgctgatga ccccaggaat
    29581 ggcgatttcg gtatacctga tcccggtatg gctgatcgtg ttaggtatcg gctatctgtt
    29641 taaagagaaa accgccaaag ccgtaaaagc gcattaatct ctctacgccc tcacccgtac
    29701 agggtgaggg caataatctt tacaccaacg ccccgtaaat cgtcagtagt gcaatcacca
    29761 ccacgaccac gaacgaggtt ttcttcgcca ttgaaaccgc tgccttcggc gtctccacct
    29821 tatcgacatg cggttcacgc gccagagaga actgcgccag acgcgttaac acctgatact
    29881 gcgaagtatg gaagtcaccc agcgaagcaa accaggccgg taacgctttc tccccatgac
    29941 cgatcaaggc atataccaca cccgcaagac gaaccggcac ccaatccagt acatgaagca
    30001 cggcatcaat gccggactgt aaacgatgat gcggcgtctg gtatcgcgcc agccagtatt
    30061 gccatgcacg caaaaacgca taccccatca gcgtaacggg tccccagatt ccccccacaa
    30121 tcaaccagaa cagcggagca agataaaaac gaaagttaat ccacagcaat gcattttgca
    30181 gctcacgcaa atactcacgt tcatcgcagc ctgccgggac gccgtgaatc atggtgagtt
    30241 cgccagccat cgtgacacgg gcatggctat cattacgtga agcagctgtc agataagcat
    30301 gataatgaag acgaacttta cctgcgccaa tacacagcaa accaatcagc agccacacca
    30361 gcagcgtggg aacgttgaac aatactccct gcaatgcgcg taacagtaaa aaagtcacgc
    30421 ccatcgcaat aatggtcatg cctaacgtgc gcccgagaga aaaatgtttc acccgccgaa
    30481 agaacgcttc aagacgatga tcaagctgcc agtgctcgcc caacttaaac aggcgctcga
    30541 aaattaacac cagtaaggtt gtaaatagcg tcatgttgtc tccttgctga ccagcgcacg
    30601 aaaccgcgcc caatcaaatg cagggccggg atcggttttc cgatccggcg caatatcaca
    30661 atggcccgtc atgttattag cgatatctgg atagcgatca atcagtgccc gcgtaaccgc
    30721 cgcaagctgt tgatactgcg catcggtata cgccagcgta tcggtgcctt caagctcaat
    30781 cccaatagaa aaatcattgc agcgttcgcg cccctgatac tgagagactc ccgcatgcca
    30841 tgcacgttta tcgaaaggaa catactggac tatttcacca tcacggcgaa tcaaacagtg
    30901 agcggagacg cgcaaatggg cgatctcagc aaagaaagga tgtgcctgcg gatcaatagt
    30961 tccagtgaat aatgcgtcga tccacggacc gccaaactcg cctggcggca ggctaatatt
    31021 gtgcaccacc agcagggtgg gtgtttcgtc atccgggcgg caatcgtaat gtggtgaggg
    31081 aacgcggcgc gcgccaacca gccacccctg ttctaacaac atgcaggatc tccttatatg
    31141 tggtgctaat acccggttca gagtagcatg tttctacctt atgattcgtt agctatctgg
    31201 agttttaaca tgccgcctcg ccgctataac cctgacaccc gacgtgacga gctgctggaa
    31261 cgcattaatc tcgatatccc cggcgcggtg gcccaggcgc tgcgggaaga tttaggcgga
    31321 acagtcgatg ccaacaatga cattacggca aaacttttac cggaaaactc tcgctctcat
    31381 gccacggtga tcacccgcga gaatggcgta ttttgcggca aacgctgggt tgaagaggtg
    31441 tttattcaac tggcaggcga cgatgtcacc ataatctggc atgtggatga cggcgatgtc
    31501 atcaacgcca atcaaccctt gttcgaactt gaaggcccat cccgcgtgct gttaacgggc
    31561 gaacgcactg cgcttaattt tgtgcaaacc ctttcaggag ttgccagtaa ggtacgccac
    31621 tatgtcgaat tactggaagg caccaacacg cagttgttgg atacgcgcaa aaccttacct
    31681 ggcctgcgtt cagctctgaa atacgcggta ctttgcggcg gcggagcgaa tcaccgtctg
    31741 gggctttctg atgccttcct gatcaaagaa aatcatatta ttgcctccgg ctcagtgcgc
    31801 caggccgtcg aaaaagcgtc ctggctgcac ccggatgcgc cagtagaagt cgaagtagag
    31861 aatctggaag aacttgatga agccctgaaa gcaggagccg atatcatcat gctggataac
    31921 ttcgaaacag aacagatgcg cgaagccgtc aaacgcacca acggcaaggc gctactggaa
    31981 gtgtctggca acgtcactga caaaacactg cgcgaatttg ccgaaacggg cgtggacttt
    32041 atctccgtcg gtgcgctaac taaacacgta caagcactcg acctttcaat gcgttttcgc
    32101 taattgctga cagatttatc cgctccatcg cgggcggata atctcccctt ccccaacttt
    32161 cttcgtaacg cctcgcaaat ttcttgtctt tcgctgcaac gaaataaaaa acctcggaag
    32221 ctgctttccc gtttcgtttt ttgcccctcg cttgtgctga tggcgtttgc caaagtagcg
    32281 ccaaccaaat caaggagcga aacagatgga caagcaacgc ggttttacac ttatcgaact
    32341 gatggtggtt attggcatca ttgccatttt aagcgccatt ggtattcccg cttatcaaaa
    32401 ctacctgcgc aaagctgcac tcaccgacat gctacaaacc tttgtgcctt accgtaccgc
    32461 cgtagagttg tgcgcgctgg aacatgg
//
LOCUS       DAJTMC010000029        31437 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.029, whole genome shotgun sequence.
ACCESSION   DAJTMC010000029 DAJTMC010000000
VERSION     DAJTMC010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31437
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.029"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(80..3361)
                     /locus_tag="OO130_001191"
     CDS             complement(80..3361)
                     /locus_tag="OO130_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HCR5364681.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKSWQAVWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPA
                     LVGLLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMT
                     GFTLRPDRAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKR
                     AVSAFPIATGTYYKVDYSAGVDISCYKNVPVTTSYMAEKSQYDFVGAWCHDEDGGLLH
                     VANHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEE
                     KRFEQYFLPYHSLGMVQNASRDAVIKLQRSKRGIEWGLYAISPLNGYRLVIREIGKCN
                     ALLDDAVALMPATAIQGVLHGINPERLTIELSDADGNIVLSYQEHQPQELPLLDVAKA
                     PLAAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNR
                     ADFPQAVAYATQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGN
                     SKAGGYYGLARLAARNGNFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARV
                     QREKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTL
                     AAEMLNALDCQRTLPLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESI
                     EECWFARHLLACFYYNKRSYNKAIAFWQRCVEMSPEFADGWRGLAIHAWNKQHDYELA
                     ARYLDNAYQLAPQDARLLFERDLLDKLSGATPEKRLARLENNLEIALKRDDMTAELLN
                     LWHLTGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDAREPQQASELL
                     HAALHYPENLSEGRLPGQTDNDIWFWQAICANAQGDETEAMRCLRLAATGDRTINIHS
                     YYNDQPVDYLFWQGMALRLLGEQQTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLL
                     SLYGDLQQQHKEKCLMVAMLASAGLGEVAQYESARAELTAINPAWPKAALFTTVMPFI
                     FNYVH"
     gene            3479..4672
                     /locus_tag="OO130_001192"
     CDS             3479..4672
                     /locus_tag="OO130_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCR5364682.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDAPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVMVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVNTQLDPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(4736..5989)
                     /gene="glyA"
                     /locus_tag="OO130_001193"
     CDS             complement(4736..5989)
                     /gene="glyA"
                     /locus_tag="OO130_001193"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HCR5364683.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            6317..7507
                     /gene="hmpA"
                     /locus_tag="OO130_001194"
     CDS             6317..7507
                     /gene="hmpA"
                     /locus_tag="OO130_001194"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HCR5364684.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISSGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIYYECFGP
                     HKVL"
     gene            complement(7552..7890)
                     /gene="glnB"
                     /locus_tag="OO130_001195"
     CDS             complement(7552..7890)
                     /gene="glnB"
                     /locus_tag="OO130_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HCR5364685.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(7951..9285)
                     /gene="glrR"
                     /locus_tag="OO130_001196"
     CDS             complement(7951..9285)
                     /gene="glrR"
                     /locus_tag="OO130_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HCR5364686.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(9275..9988)
                     /gene="qseG"
                     /locus_tag="OO130_001197"
     CDS             complement(9275..9988)
                     /gene="qseG"
                     /locus_tag="OO130_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HCR5364687.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGCWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(10153..11580)
                     /gene="qseE"
                     /locus_tag="OO130_001198"
     CDS             complement(10153..11580)
                     /gene="qseE"
                     /locus_tag="OO130_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HCR5364688.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSS
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(12138..16025)
                     /gene="purL"
                     /locus_tag="OO130_001199"
                     /gene_synonym="purI"
     CDS             complement(12138..16025)
                     /gene="purL"
                     /locus_tag="OO130_001199"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HCR5364689.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFDLDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFRMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRILGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRILHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGINADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            16286..17842
                     /gene="mltF"
                     /locus_tag="OO130_001200"
     CDS             16286..17842
                     /gene="mltF"
                     /locus_tag="OO130_001200"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HCR5364690.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLCNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(17839..18375)
                     /gene="tadA"
                     /locus_tag="OO130_001201"
     CDS             complement(17839..18375)
                     /gene="tadA"
                     /locus_tag="OO130_001201"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HCR5364691.1"
                     /translation="MRRAFITGVFFLSEVEFSHEYWMRHALTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIKAQKKAQSSTD"
     gene            complement(18400..19035)
                     /gene="yfhb"
                     /locus_tag="OO130_001202"
     CDS             complement(18400..19035)
                     /gene="yfhb"
                     /locus_tag="OO130_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HCR5364692.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDKPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            19244..20092
                     /locus_tag="OO130_001203"
     CDS             19244..20092
                     /locus_tag="OO130_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCR5364693.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            20148..20408
                     /gene="yfhL"
                     /locus_tag="OO130_001204"
     CDS             20148..20408
                     /gene="yfhL"
                     /locus_tag="OO130_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HCR5364694.1"
                     /translation="MALLITKKCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            complement(20602..20682)
                     /gene="shoB"
                     /locus_tag="OO130_001205"
     CDS             complement(20602..20682)
                     /gene="shoB"
                     /locus_tag="OO130_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HCR5364695.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(21102..21482)
                     /locus_tag="OO130_001206"
     CDS             complement(21102..21482)
                     /locus_tag="OO130_001206"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HCR5364696.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            complement(21482..22213)
                     /gene="pdxJ"
                     /locus_tag="OO130_001207"
     CDS             complement(21482..22213)
                     /gene="pdxJ"
                     /locus_tag="OO130_001207"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HCR5364697.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKMRDACKRLTDAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            complement(22225..22953)
                     /gene="recO"
                     /locus_tag="OO130_001208"
     CDS             complement(22225..22953)
                     /gene="recO"
                     /locus_tag="OO130_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HCR5364698.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGATGTPEPALRRFELALLGHLGYGVNFTYCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            complement(22965..23870)
                     /gene="era"
                     /locus_tag="OO130_001209"
     CDS             complement(22965..23870)
                     /gene="era"
                     /locus_tag="OO130_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HCR5364699.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLRDGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            complement(23867..24547)
                     /gene="rnc"
                     /locus_tag="OO130_001210"
     CDS             complement(23867..24547)
                     /gene="rnc"
                     /locus_tag="OO130_001210"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HCR5364700.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            complement(24820..25794)
                     /gene="lepB"
                     /locus_tag="OO130_001211"
     CDS             complement(24820..25794)
                     /gene="lepB"
                     /locus_tag="OO130_001211"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCR5364701.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            complement(25810..27609)
                     /gene="lepA"
                     /locus_tag="OO130_001212"
     CDS             complement(25810..27609)
                     /gene="lepA"
                     /locus_tag="OO130_001212"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HCR5364702.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            complement(27807..28286)
                     /gene="rseC"
                     /locus_tag="OO130_001213"
     CDS             complement(27807..28286)
                     /gene="rseC"
                     /locus_tag="OO130_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HCR5364703.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            complement(28283..29239)
                     /gene="rseB"
                     /locus_tag="OO130_001214"
     CDS             complement(28283..29239)
                     /gene="rseB"
                     /locus_tag="OO130_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HCR5364704.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGDEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            complement(29239..29889)
                     /gene="rseA"
                     /locus_tag="OO130_001215"
     CDS             complement(29239..29889)
                     /gene="rseA"
                     /locus_tag="OO130_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HCR5364705.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            complement(29922..30497)
                     /gene="rpoE"
                     /locus_tag="OO130_001216"
     CDS             complement(29922..30497)
                     /gene="rpoE"
                     /locus_tag="OO130_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HCR5364706.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            complement(30494..30649)
                     /gene="rseD"
                     /locus_tag="OO130_001217"
     CDS             complement(30494..30649)
                     /gene="rseD"
                     /locus_tag="OO130_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HCR5364707.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            30905..>31437
                     /locus_tag="OO130_001218"
     CDS             30905..>31437
                     /locus_tag="OO130_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR5364708.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHVQPNGEESYHLTREGGHSHRRILHAADATGREVQSTLVSKAQNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRR"
BASE COUNT         7528 a         8419 c         7926 g         7564 t
ORIGIN      
        1 cacgcaagca ccttaaaatc accatcatac actaaatcag taagttggca gcattaccaa
       61 atattacagt taatagggtt tagtgaacgt agttaaaaat aaaaggcatc acagtggtga
      121 ataatgccgc tttcggccag gccggattaa tcgccgtcaa ttcagcgcga gcagactcat
      181 attgcgcaac ctccccgagt cccgcggacg ccagcatcgc caccatcagg catttttctt
      241 tatgctgctg ttgtaaatcg ccatacagcg ataacaggtc aggttgtgaa acagcaaaga
      301 aatccgcctc aatactggtt ttcgccatct cttgcgccca ctgtttcatt tcactaaaca
      361 gttgctgtgc ggtttgctgt tcacccagca gtcgcagcgc cattccttgc cagaagagat
      421 aatcaaccgg ctgatcgttg taataactgt ggatgttaat ggtgcgatcg ccggtcgccg
      481 ccagacgtaa acaacgcatc gcttcagttt catcgccctg cgcgttggcg catatcgcct
      541 gccagaacca gatgtcgtta tcagtttgcc ccggtaaacg gccttcgctt aaattctccg
      601 gataatgcag cgcggcatga agcagttcgc tggcctgctg cggctctctg gcatcaagat
      661 gctgccaggc gcgtaataac tggttgagga taaactgact ggtgaccttc ccttccccgc
      721 cttcccacgg gtggaattta cgcgtggcga gaatgtccgc cgctttgtct gcctgacccg
      781 tgagatgcca caaattgagc agttctgcgg tcatgtcgtc gcgtttcagc gcaatttcca
      841 gattattttc cagacgcgcc agtcgtttct ccggtgtggc tccacttaac ttatcaagca
      901 aatcccgttc gaaaagcaga cgtgcatcct gcggcgcaag ctgataagca ttatcaagat
      961 aacgcgcggc cagctcataa tcgtgttgct tattccacgc atggatcgct aacccgcgcc
     1021 agccgtcggc aaactccggc gacatctcta cgcaacgttg ccaaaaggca atggctttgt
     1081 tgtagctacg tttgttgtag tagaagcagg ccagtaaatg gcgagcaaac cagcactctt
     1141 caatactctc cagcgccgcc acttcttcca gcgtattcgg gaaacggaca aactgcggga
     1201 agacatcaat ggcttttgcg accagttcgc cacgttcggc tttcggcagc aagctggctt
     1261 gcaggtaaag cggcagcgtg cgctggcagt ccagtgcgtt cagcatctct gccgccaggg
     1321 tgggcattcc ccagttaatc agttgcccgg cggtcatcag ggcgttaacg tcgcgtccct
     1381 gacacagacc gcgccactgc gcgagagctg attcgctacg accatcgaac cagttcagcc
     1441 accacagagt ggcgttcagc ggataatcgc gcagcagttt ctcgcgctgc acacgcgcgt
     1501 tgtcctgacg accacttaac accagcagca gattatgcag gcaaagcact tcctgattgg
     1561 ttgggcaggc gcgaagactt tgttggcaaa aatccagacc agcgtcgaag ttaccattac
     1621 gcgccgccag tcgtgccagg ccataatagc caccggcttt gctgttgccg ctccagaccg
     1681 cacgccagaa atcctcttcg gcttgttgat attgtccctg acgttcgtaa gcactggcgc
     1741 gaatcaaact cgcctgtccg cactgcggat ttttgttcag cgcatgtgcg cgtttcagag
     1801 cctgagtggc atacgccacc gcttgcggga aatcggcgcg gttatattcc agcatcgcca
     1861 gcgccaggtt acagcggtaa tccagcggat ccagcgccac gccgcgcagg tagtaatcga
     1921 acggtgaacg gctggcgtga tgatattgct ccagatgctg accgataaac caggcttcat
     1981 ctgtactggt aatgtcttgt gctgccagtg gcgctttggc gacgtccagc aacggcaact
     2041 cttgcggctg atgttcctga taactcagta caatattgcc gtcggcatcg gagagctcaa
     2101 tggtcagcct ttcaggattg ataccgtgca acacgccctg gatggcggtc gcaggcatca
     2161 gtgccacggc atcatcaagt aacgcgttgc atttgccgat ttcgcggatc accaggcggt
     2221 atccgttcaa cggagagatg gcatacagcc cccactcaat cccccgctta ctacgctgga
     2281 gttttatcac cgcatcgcgg gaggcatttt gcaccatgcc caaagaatga taaggcagga
     2341 aatactgctc aaaacgcttc tcttcgtaag catcaagcca ggtaaaatcg ggctggttat
     2401 cggcaaaaat accggtcatc agttcgatat acgggccgtt attgtcggtc agactcttat
     2461 cccacgcctg gccaaattca ctgtgtcccc aactccactg ttttttacct ggcgcaatat
     2521 ggtggttggc aacgtgcagc aaaccgccat cttcatcgtg acaccacgcg ccgacaaaat
     2581 cgtactgcga tttttcggcc atatatgagg ttgtaacagg cacattttta tagcaagaaa
     2641 tgtccactcc ggcggagtag tccactttgt agtaagtgcc ggtggcgatg gggaaagcgg
     2701 agacggcccg tttgccgtga tcaaacaccg cagtcacatc cggtgggaaa acgctctgat
     2761 gcccttcccc ccctttcact gccgggttgg cccaccacaa gaaatgacgc ggcgtggcgt
     2821 taccgttata gacgcggctg gcgatttcca gcgccgcccg gtcagggcgc agggtgaaac
     2881 ctgtcatcac ctgtaaacca tgcatcggct ccgtttcgcc tacccacacc gtctgtgcac
     2941 cgtcttcatg ggcttcgagg gtgaaatcaa cgggcataaa ggtggtcggg cgatgatgtt
     3001 gcggccagtt aaactcaatc ccaccggaaa tccacggccc cagcagcccc accagcgcag
     3061 gtttaatgac ttcattgtga taaacaaaat cgcgctgttt cactttatcc cacgcgcgat
     3121 gcacccgacc gcccagttcc ggcaggatca tcactttgat gtagtcgttt tccagccaca
     3181 ccgcctgcca ggatttcagg gttttctgct cgctcagcgt atcggtcacg ccgtagggat
     3241 aaaccgcgcc ggacgatccc tgataaacgc gattttccag gaacatggga tgtatatcct
     3301 gcggcccggt ttcataggtc gggatctcaa cgcgctcttg ccacactttt actggagtca
     3361 tggtgccctc aatattaaca agacatactg aattaaaaga tttgtggcag tgtattgaac
     3421 aatctggcaa tgttttcgcg gaataatcac gcaattaact aaacaaggtt tagtgaagat
     3481 gagagcctgc attaacaatc aacagattcg ccaccataac aaatgcgtga ttctggaact
     3541 gctgtaccgg caaaagcgcg ccaataaatc aacgctggcc cggctggcgc aaatttcgat
     3601 tccggcagtc agtaatattt tgcaggaact ggaaagcgaa aaacgggtgg tgaatattga
     3661 cgatgaaagc cagacgcgcg ggcatagtag cggtacatgg ctgattgcgc cggaaggtga
     3721 ctggacgctg tgcctgaacg tgacgcccac cagtattgag tgtcaggttg ctaatgcttg
     3781 tttaagtccg aaaggtgaat ttgagtattt acagattgat gcaccgacgc cgcaggcgct
     3841 gctgtccgaa atcgaaaaat gctggcatcg ccaccgtaaa ttgtggccgg accgcaccat
     3901 caatctggcg ctggcaatcc acggtcaggt tgatccggtg accggcgtgt cgcaaaccat
     3961 gccgcaagcg ccgtggacaa cgccggttga ggtgaagtat ctgctggaag agaagctggg
     4021 cattcgggtg atggtcgata atgactgcgt gatgctggcg ctggcggaga aatggcaaaa
     4081 taattcgcag gaacgggatt tctgcgtgat caacgttgat tacggcattg gctcgtcgtt
     4141 cgtgattaac gagcaaattt atcgcggcag tttgtatggt agcggacaaa ttggtcacac
     4201 catcgttaat ccggatggcg tcgtctgcga ctgtggacgt tacggctgcc tggaaaccgt
     4261 cgcctcgtta agtgcattaa aaaaacaggc gcgggtatgg ctaaaatcac aaccggttaa
     4321 tactcagctt gatcctgaaa aactgacaac cgcgcagtta atcgctgcct ggcagagtgg
     4381 agaaccgtgg atcaccagct gggttgatcg ctctgccaat gccattggtt tgagtctgta
     4441 taacttcctc aacatcctca atattaatca gatttggttg tacggtcgca gttgtgcctt
     4501 tggtgagaac tggcttaata ctattattcg ccagacagga tttaacccgt tcgaccgcga
     4561 cgaaggaccg agcgtgaaag cgacgcaaat tggcttcggg caattaagcc gcgcacaaca
     4621 ggtgctggga attggctatt tgtatgttga ggcgcagtta cgacagattt gatggcgcga
     4681 taacgtagaa aggcttcccg aaggaagcct tttttcaaca gattaaccgt ttcgcttatg
     4741 cgtaaaccgg gtaacgtgcg cagatgtcga gaactttacc tttgatgcgc tcgataacgg
     4801 cttcatcatt gatgctgtcc agcacgtcac acatccagcc agccagttct ttcgcttctg
     4861 cttctttgaa gccgcgacgc gtaattgccg gagtacccac gcgaataccg gaggtcacaa
     4921 acgggctctt cggatcgttc ggtacgctgt ttttgttgac ggtgatgtta gcacggccca
     4981 gagcggcgtc tgcttcttta ccggtcaggt ttttatcaac cagatcaacc aggaacaggt
     5041 ggttatcagt gccgccggaa accactttgt agccgcgctc gaggaacact tctaccatcg
     5101 ctttagcgtt tttcgcgacc tgctgctggt aagttttgaa ctcaggctcc atcgcttctt
     5161 tcagagcaac cgctttaccg gcgattacgt gcatcaacgg accgccctga ccaccaggga
     5221 aaacggcaga gttcagtttt ttgtacagct cttcgctacc acctttcgcc aggatcaggc
     5281 cgccgcgcgg acccgccagg gttttgtgag tggtggtagt aacaacgtga gcatgaggaa
     5341 ccgggttcgg gtagacgcca gcagcaacca ggcccgcaac gtgcgccata tcaacgaaca
     5401 ggtaagcacc gatgctgtca gcgatttcac gcattttcgc ccagtccacc acgccggaat
     5461 atgcagagaa accaccgata atcattttcg gcttgtgttc tttggcttgt ttttccagat
     5521 cggcgtagtc gatatgaccg gtagcatcga taccgtaagg aacgatgttg tacagtttac
     5581 cggagaagtt aaccggagaa ccgtgagtca ggtgaccgcc atgcgccagg ttcataccca
     5641 gaacggtatc acctggttcc agcagcgcgg tgtagaccgc aaagttagcc tgggagccgg
     5701 agtgcggctg gacgttagcg tagtcagcgc cgaacagttc tttcgcacga tcgatcgcca
     5761 gttgttcaac gatatcaaca tactcgcaac cgccgtagta gcgtttgccc ggataacctt
     5821 cagcatattt gttggtcagc tgagaaccct gcgcctgcat tacgcgcggg ctggtgtagt
     5881 tttcggaggc gatcagttcg atgtgctctt cctgacgtac tttttcctgc tccatagcct
     5941 gccacagttc ggcatcataa tcggcaatgt tcatttcacg ctttaacatc cgcatctcct
     6001 gactcagcta acaataaaat ttttggcctt tataggcggt cctgttggac aacggcgaac
     6061 agtataaccg aatcattgtg cgataacagg tcttgacaaa ggaatttacg caaacgatta
     6121 ccttcaggct acgcaaggct ttggagaata aagagcttgc aaccggaaac ggatttcttt
     6181 tcaggtttgt gatgcaaatt tttcacttca tcacattctt tctgaaaaac accaaagaac
     6241 catttacatt gcagggctat tttttataag atgcatttga aatacatcaa ttaagatgca
     6301 aaaaaaggaa gaccatatgc ttgacgctca aaccatcgct acagtaaaag ccaccatccc
     6361 tttactggtg gaaacggggc caaagttaac cgcccatttc tacgaccgta tgtttactca
     6421 taacccagaa ctcaaagaaa tttttaacat gagtaaccag cgtaatggcg atcaacgtga
     6481 agccctgttt aacgctattg ccgcctacgc cagtaatatt gaaaacctgc ctgcgctgct
     6541 gccagcggta gaaaaaatcg cgcagaagca caccagcttc cagatcaaac cggaacagta
     6601 caacatcgtc ggtgaacacc tgttggcaac gctggacgaa atgttcagcc cggggcagga
     6661 agtgctggac gcgtggggta aagcctatgg tgtactggct aatgtattta tcaatcgcga
     6721 ggcggaaatc tataacgaaa acgccagcaa agccggtggt tgggaaggta ctcgcgattt
     6781 ccgcattgtg gctaaaacac cgcgcagcgc gcttatcacc agcttcgaac tggagccggt
     6841 cgacggtggc gcagtggcag aataccgtcc ggggcaatat ctcggcgtct ggctgaagcc
     6901 ggaaggtttc ccgcatcagg aaattcgtca gtactctttg actcgcaaac cggatggcaa
     6961 aggctatcgt attgcggtga aacgcgaaga gggtgggcag gtatccaact ggttgcacaa
     7021 tcacgccaat gttggcgatg tcgtgaaact ggtcgctccg gcaggtgatt tctttatggc
     7081 tgtcgcagat gacacaccag tgacgttaat ctcttccggt gttggtcaaa cgccaatgct
     7141 ggcaatgctc gacacgctgg caaaagcagg ccacacagca caagtgaact ggttccatgc
     7201 ggcagaaaat ggcgatgttc acgcctttgc cgatgaagtt aaggaactgg ggcagtcact
     7261 gccgcgcttt accgcgcaca cctggtatcg tcagccgagc gaagccgatc gcgctaaagg
     7321 tcagtttgat agcgaaggtc tgatggattt gagcaaactg gaaggtgcgt tcagcgatcc
     7381 gacaatgcag ttctatctct gcggcccggt tggcttcatg cagtttaccg cgaaacagtt
     7441 agtggatctg ggcgtgaagc aggaaaacat ttattacgaa tgctttggcc cgcataaggt
     7501 gctgtaattt gatgttgccg gatggaaaca tccggcaacc cttgacgcgg tttaaattgc
     7561 cgcgtcgtcc tcttcaccgg tacggatgcg aatgacccgt gccacgtcaa agacgaagat
     7621 tttaccgtca ccgattttgc cggtttgcgc cgtgcgaata atggtatcga cacaggtatc
     7681 gacaatgtcg tccggtacga caatctcaat tttcactttc ggcagaaaat ccaccatata
     7741 ctccgcgccg cggtacagct cggtatggcc tttctggcga ccaaagcctt tcacttcggt
     7801 caccgtcatg ccggtaatac cgacttcggc cagtgcttcg cggacatcgt ccagcttgaa
     7861 gggttttata atcgcatcaa tctttttcat gctattcctt gaaaaggtcg cctgtctttt
     7921 gatctgctaa acgtaacaca taacgccaat tcattccttg aaatcgtttg catccagctc
     7981 gtgtcgggaa agcagtttat aaaattctgt ccggttgcgc cccgccattc tcgccgcgtg
     8041 ggtgacgttg cctttggtaa tttgcagcag cttacgcaaa tagttgagtt caaactgatt
     8101 acgtgcctca acaaaggttg gcagcgccgt gttttcaccc tccagcgcct gctctactaa
     8161 tgcatcgcta ataaccggag atgaggtcag cgccacgcac tgttcaatca cgttgaccaa
     8221 ctggcgcaca ttacccggcc agctagcggt catcaggcgt ttcatcgcat cagtggagaa
     8281 cgcgcggaca aacggtttat gtcgctctgc cgcctggcgc aacaggtgat ttgccagaag
     8341 cggaatgtct tctgtgcgct ctgccagagc cggaattttc aggctgacaa cgttgaggcg
     8401 gtaatagaga tcttcgcgga attccccgcg cgccatcgct tttggcagat cacggtgagt
     8461 ggcagaaata atccgcacat tgatatcaat atcgcggtta ctgcccagcg ggcgcacttt
     8521 acgctcctgc aacacgcgca gcagtttgac ctgtaacggc gcaggcatat cgccaatttc
     8581 atcgagaaat agcgttccgc cttccgccgc ctggaataaa ccttcgcgat tgctgacagc
     8641 gccagtaaac gcgccacgcg catgaccaaa cagctccgac tccagcaatt gctcgggcaa
     8701 tgcgccacag ttaatagcaa taaatggttt gctgttgcgc gggctggcgt tgtggatagc
     8761 ctgggcgaaa atctctttcc cggtgccgct ctgaccgtta atcaaaacgc tgacgtctga
     8821 ttgcgccacc agccgcgcct gttccagcaa acgcagcatc agcgggctgc gggtgacaat
     8881 tgcctcgcgc cagcgttcat cggtggctgg cgcggattgc tccagcgcat cgtcaattgc
     8941 ctgatatagc gcgtctttgt cgacaggctt ggtgaggaaa ctaaaaacgc cctgctgtgt
     9001 tgcagcaacg gcatcgggga tagaaccatg cgcagtaaga ataattactg gcattcccgg
     9061 ctgcactttc tggatttcag caaatagctg cataccgtcc atttcatcca tccgcaggtc
     9121 gctgataact aaatctactt tttcgcgatt cagtacccgt aatccttcag cgccactttc
     9181 cgccgtgacc acactgtagc cttcgctggt caggcgcagg ccaagcagtt tcagcaatcc
     9241 cggatcgtca tcgaccaata ataaatgcgc aggtttatgg ctcatcagga gtgacctcat
     9301 gggtggatgg cgcgggcttt tcactctcgt gcggcgtatc cggcgagaaa tttccagccg
     9361 gtttgcgggt cgagagctgg cgttcaatat cggtcaggtt ttccagcttg cgggtggtga
     9421 gttccagttg ctgctgtaga acgtgatgtt gctggcgcaa tgtatccagc tcgctgtcgc
     9481 tggactgctg gagtttactg taacgttggc gctcttccgc cagttgcagt tgcagcgcct
     9541 gaccgtcgcg ccagagttga tacagtgggc gaacctgtgc cgggatctcg gtacttaacg
     9601 cttcaatacg cgcgaccagc tggcggcgct catacggcgt aattttggcg tcggcgagca
     9661 aaatcccttg tttaaaggta ttttgccagc agccgtcgtc atattggcga gcttgctgac
     9721 gcgactgcgc aggcattaaa cgatcagcac aatccatcgc ccgcagccag taaagcggat
     9781 tggtttcggt tgatttacct tgcagcgccc agatgtcgct acattcagta gaaagatagt
     9841 cagccagttg ataaaccgga attttttctt ctgctggcgt atcaatggct ggcttattgt
     9901 gattctgcac gcaacccagc aatgccagac atggcagccc tgccagccac agccgtcggg
     9961 gcaataatcg ttgaaaaatg tgtcgcatat tcaccagact taaagcctat cccagtgggc
    10021 gtaattgttg cagacagtct ggacatggac agcgcggaga aaccggagcg tacatatcgt
    10081 acgtgaagat ttcgagcact gcccggagcc gaactgacaa ataaaatagc ctgataggat
    10141 aggctgttag cattatttcg tgtttttcga cgacggtaat tcaatgcgga aacaaacgtc
    10201 ttgcccgctc tcgtcgacca gatacagttc cccttgcata cggcgaatac aatccctggc
    10261 aatgcttaat cccagaccgc tgcccttcac cgcccctttt cgctggtggc ttccctgaaa
    10321 aaagggttcg aagatcatgg cgcgttcctc ttgcggaatg ggcgtgcctg tattgatgac
    10381 atcaatataa acccgcgcac catgtgaact gctgcgaagg caaatgttac cggattcagc
    10441 cccgtagtgc accgcattgg agtaaagatt atccagtacg ctcatcagca gcattggctc
    10501 cgccaggcaa gctgttgctt tgagatcgac gtcggtatgc atcattttag cccgtgcggg
    10561 caggctatga gcagaaacca ctgtctccac cagcggtgct aattcaacat tctccagttc
    10621 caccgcactg tccgcctgtt tacggttgta atcaagcagt tgttcgatca gtttttgcaa
    10681 attgcggctg ctgctatcaa gaatgctcac cacctctttt tgctctggcg taagcggccc
    10741 gacaacctgg tcagccagta attcagtgcc ctcgcgcata ctcgccagtg gcgtttttaa
    10801 ttcatgagat aaatgtctta aaaattgatg gcgttgggat tccagccatg acaggcgctc
    10861 acttaaccaa agaatacgtt gcccaaccga gcgtaactcg ctcggtccac tgaacgaaac
    10921 gctattgccc agagaacgcc cttcccccag acggttgatc atgcgctcga tatttttcac
    10981 cggcccgata atcatccgcg tgaaaagtag caccatcacc agactcacca gaaatagcac
    11041 cagcgattgc caaccaaaat attgcccacg ttcggcgatt tcacgctgaa gttgctgccc
    11101 acgagagaac accactgtgc gcgtggcctg caccatttcg gtattggcac tggcaaaggc
    11161 ttcaagacgc gcggcggcgg cagcatcggg accactgttg ttacactgaa gttgggccag
    11221 attgttcaaa tcctgacgca atgcctggta aagtttatcg tctggcagta cgcctgcgtg
    11281 ggcatcgagc atttcgctgt aacgcttgcg ctggctttga taaaccttcg caagcgtagg
    11341 gtcgtccagc acgcaatact gacggtagct acgctccatc tccagcgccg cgttggtcat
    11401 cgcttcacta cgccgggcat caataagcgt agtgcggtta accagcgccg cctgatcgct
    11461 aagcgcattc aggctttgcc aggcttgcca tgccagcacc aacaggggta gtagaattag
    11521 caaaaatgcc agcattacca gttgtcgtaa tgagcgggga aaaacgggcc agcgtttcaa
    11581 ggtgttactc tcgtcagacg cgaatagcct gatgctaacc gaggggaagt tcagatacaa
    11641 caaagccggg aattacccgg ctttgttatg gaataaggcg gtgcctaact cgacgtttcg
    11701 cccgatggtt gatatagcta cgctgatatc agaagttgga cggcaggcac cttgttgtgc
    11761 gtcattcgaa ttttatgtag cacgtcccga aggggctgac ataagtcggt gaatgagcca
    11821 ctgcttacta ttatgcagta actgtgccaa taaagaaaat agtttggtaa cgtactgatt
    11881 atacgttgct ttgagggatt tatggcttct tcgctatttg aatgtaaatc agccactgtg
    11941 tcgctaaaaa gagacagctt aagataaact tattagatta caataaaatc aatcacttaa
    12001 gtgtcgccag aaagcgacac ggtaaaccac ccattgtcgt gatttacaga cacaaaaaag
    12061 ctcccggagt tgggagctta tgatagtggt tggtgcttaa tgccgcatcc gggctgcaaa
    12121 accaatgaac tgatgaatta ccccaactgc ttacgcgcat tgcggaaaat gcgcatccat
    12181 gggccatcct cgccccagtt ttccggatgc caggagttgc tgacagtacg gaaaacacgt
    12241 tccgggtgcg gcatcataat ggtgactcga ccactttcag tcgtgactgc cgtaataccg
    12301 ttcggtgaac cgttcgggtt ggccgggtag gtttcagtga ctttgccgaa gttatcgaca
    12361 tagcgcagtg ccaccagccc tttgctttcc agtgccgcca gatgcgccgc atcacgcact
    12421 tccacgcgcc cttcaccatg agagacagca atcggcattt gcgagcccac catcccctgc
    12481 aacaacagag acgggctttg ggttacttca accaggctga aacgcgcttc aaagcgatcg
    12541 gaggtattgc gcacaaaacg tggccacaac tcactacctg ggatcagttc gcgcaggtta
    12601 gacatcatct ggcaaccgtt acataccccc agcgccagcg tttgcggacg gtggaagaag
    12661 gttgcaaact catcgcgtac acggtcattg aacaggattg acttcgccca accttcaccg
    12721 gcacccagca catcaccgta ggagaaacca ccgcacgcga ccagggcgtg gaaatcttcc
    12781 aggcccgtgc gtccggtcag caggtcactc atatgcacgt cgatagcatc aaagcctgca
    12841 cggtggaaag ctgccgccat ttcaacatgc gagttcacgc cctgctcacg cagtacggca
    12901 actttcggac gtgcgccagt ggcaatatac ggtgctgcca catcttcgtt gatatcgaac
    12961 gacagtttta cattcaggcc cggatcggcg tcgttagatt tcgcctgatg ctcctgatcg
    13021 gcacactccg ggttgtcacg caggcgctgc atctgccagg tagtttctgc ccaccagaca
    13081 cgcaacgtgg tgcggctttc gctgaataca gtctgcccgt tggcggtaat cacaaaacgg
    13141 tcaccggaaa ccgcctgccc tacataatgg acacaatcag caagcccatg ctgtgccagt
    13201 acggactcga ccgcttcacg gtcagcggca cgaacctgaa tcaccgcacc cagttcttcg
    13261 ttaaacaacg ccgccaggcg atcgtcgccc agagaggcaa tatccgcgtt aatgccgcaa
    13321 tgaccagcaa aggccatttc cgccagcgtt accagcaggc cgccatcaga gcggtcgtgg
    13381 tatgcgagca gcttacgctg tgcaaccagc gcctgaatcg cgtcatagaa gcctttcagt
    13441 tgcgcgacgt cgcgtacatc tgccggtttg tcgccaagct gacgataaac ctgcgccagc
    13501 gccgttgcgc ccagcgcgtt attgcctttg cccaaatcaa tcagcaatag tgcgttatct
    13561 tcggtagaaa gctgtggcgt gatggtgtga cgcacatctt ccacgcgggc aaatgcagaa
    13621 atcaccagcg acagcggcga cgtcatttcg cgctcttcgt taccttcctg ccagcgggtt
    13681 ttcatcgaca tagagtcttt acccaccggg atcgtcaggc ccagcgccgg acaaagctct
    13741 tcgcccacgg ctttaacggc ttcatacagg cccgcatctt caccagggtg gcctgccgcc
    13801 gccatccagt tggcggaaag tttaatgcgt ttgatatcgc caatctgtgt tgcggcaata
    13861 ttggttaacg cttcaccgac cgccagacgg gcagaggcgg cgaaatccag cagcgcaacc
    13921 ggcgcacgct cgccaatcgc catcgcttca ccgtagtagc tgtcgaggct ggcggtagtg
    13981 accgcgcagt tagcgaccgg cacctgccac ggacccacca tctgatcgcg cgctaccatg
    14041 ccagttacgc tgcggtcacc gatggtcacc aggaaagttt tctccgccac ggtcggcaga
    14101 tgcagtatgc gtttcaccgc gtctgcaatg gtgatccctt cacggaccag cgcatcgccc
    14161 ttcgctttca gcgtttgtac atcgcgggtc atcttcggcg ttttaccaag caggacgtcc
    14221 agcggcagat cgatcggctg attatcaaaa tgacgatcgt gtagagaaag atgcagttct
    14281 tcggtcgctt caccaatcac cgcgtagggt gcgcgctcac gcttacacag ttcgtcaaac
    14341 agcggtaatt gatcggcagc aaccgccagc acgtagcgtt cctgggattc gttacaccag
    14401 atttccagcg ggctcatgcc tggctcgtcg ctcagaatgt cgcgcagttc aaatttaccg
    14461 ccacgcccgc cgtcgctcac cagttccggc atagcgttag aaagaccgcc tgcgccaacg
    14521 tcgtggataa acagtattgg gttggcatca ccaagctgcc agcaacggtc aatcacttcc
    14581 tggcagcgac gctccatctc cgggttgtcg cgctgtacgg aagcaaagtc gaggtcggca
    14641 tcagactgac cagacgccat ggaagacgcc gcgccgccgc caagaccgat gttcattgcc
    14701 gggccgccga gaacgaccag cttcgcgcca acgttgatct cgcctttttg tacgtgatcg
    14761 gcgcgaatgt tgccgatccc gcccgccagc atgatcggtt tgtgataacc gcgcagctct
    14821 tcgccgttgt ggctgttcac tttttcttca taagtacgga agtagccgtt cagtgccgga
    14881 cgaccaaatt cgttgttaaa cgccgcaccg cccagcgggc cttcggtcat gatgtccagc
    14941 gcggtgacaa tgcgctcagg cttaccgaaa tcttcttccc acggctgttc gaagccaaga
    15001 attcgcaggt tggaaacgga gaaaccaacc aaacccgctt tcggcttcgc gccgcgcccg
    15061 gtggcacctt catcgcggat ttcaccgccg gaaccggtcg ccgcgcccgg ccacggagaa
    15121 atcgccgtcg ggtggttgtg agtttcgact ttcatcagaa tatgcgccgg ttcctgatgg
    15181 aaatcatagc ggcccgtttc gtggtcagca aagtagcggc ccacttcaga accttccatt
    15241 acggcggcgt tatctttata agcagagaga acgtgatctg gcgtggtttc gaaagtattt
    15301 ttgatcatcc tgaacagcga tttcggctgt tgttcaccgt cgataatcca gtcggcgtta
    15361 aaaattttgt ggcggcagtg ctcagagttc gcctgagcga acatatacag ctcgatgtcg
    15421 ttcgggttac gaccaagctt tgtgaaagca tcctgcagat agtcaatttc atcttccgcc
    15481 agagccaggc caagacgcag gttagcgtcg atcagcgcct gacggccctg ccccagcaaa
    15541 tcaacgcttg tgaccggagt cggttgatgg tgggcaaaca actgctctgc atcatctaaa
    15601 tcaaaaaaga cagtttccat catgcggtcg tgcagttcag cggtaacctg ctgccattgt
    15661 tcattggtca gcgtaccggc ttctatatag taagcaacgc cgcgctcaag gcggtttacc
    15721 tgttgtagcc cgcagttatg ggcaatatcg gtcgctttcg aagaccaggg agagatggtg
    15781 ccaggacgcg gggtcaccag caggagtttg ccttgcgggg cgtggctggc gagtgccgga
    15841 ccatatttca gcaggcgttc aagttgtgcg tgctcatcat cgtttaacgg cgcattgagg
    15901 tcagcaaaat ggacatactc ggcgtaaata ttgtgaaccg ggagcctggc agcctgaaaa
    15961 cgtgccagca gtttgttgat tcggaatgcc gacagtgcag gcgaaccacg cagaatttcc
    16021 atcataagtc tctcgtcttc taagctttcg gtgtacccaa gggggggggg aaacgggcgt
    16081 cattataaag aatctgatgc gctgacgaaa ccgtttgcgt ggaaataaaa tcaccatcgt
    16141 gaattagcaa cgcgtgccgc caatggctgt aataagttgc catctggcgc aggtttacgc
    16201 aaaatgccgc tcatttatga gtaaaccttt cactattatt acgttttttc aagctgggac
    16261 gcgcacgaca cagagaatta actaattgaa aaaattaaag attaattatc tgttcatcgg
    16321 cattctggca ctgctgctcg cggtcgctct ctggccatcc attccctggt ttggtaaagc
    16381 cgacaaccgt atcgccgcca ttcaagcgcg gggagagttg cgtgtgagca ccattcatac
    16441 tcccctgact tataacgaaa tcaacgggaa accttttggc ctggattacg aactggcgaa
    16501 acagtttgcc gattacctcg gcgtaaaact gaaagtgacc gtgcggcaga atatcagcca
    16561 gctgtttgac gaccttgata atggtaacgc cgacctgctg gcggcaggac tggtctataa
    16621 cagtgagcgg gtaaaaaatt atcagcctgg ccctacctat tattccgtgt cacaacaact
    16681 ggtttataaa gtgggtcagt atcgcccacg tacgctgtgc aacctgacgg cggagcaact
    16741 caccgttgca ccgggtcatg tggtggttaa cgatctccag accctgaaag aaacaaaatt
    16801 cccggaatta agctggaagg tagacgacaa aaaaggctct gcggaattaa tggaagatgt
    16861 catcgaagga aaactcgatt acaccattgc tgattctgtc gccatcagcc tgtttcagcg
    16921 cgttcacccg gagctcgccg tagcgctcga tatcaccgat gaacaaccgg tgacttggtt
    16981 tagcccgtta gatggcgata ataccctttc cgccgccctg ctcgacttct tcaacgaaat
    17041 gaatgaagac ggtacgctgg cacgcattga agagaaatac ctggggcatg gcgatgattt
    17101 tgattacgtc gatacgcgca catttttacg cgccgtcgat gcggtactgc cgcagttaaa
    17161 gcccctgttt gagaaatacg ccgaagaaat tgactggcgt ttgctggccg ctattgctta
    17221 tcaggaatcg cactgggatg cacaggccac ttcaccgacg ggtgtgcgcg gcatgatgat
    17281 gttaaccaaa aataccgcgc aaagcctcgg cattacggat cgtaccgatg ccgaacagag
    17341 catcagcggt ggcgtgcgtt atttgcagga tatgatgagt aaagtgccgg aaagtgtgcc
    17401 ggagaacgag cggatctggt ttgccctcgc tgcgtacaat atgggctatg cgcatatgct
    17461 ggatgcccgc gccctgacgg caaaaaccaa agggaatcct gacagttggg ctgacgtaaa
    17521 acagcgtctg cctttactta gccagaaacc ctattacagc aagctgactt acggctatgc
    17581 tcgtggacat gaagcctacg cttatgtcga aaatattcgt aagtatcaaa ttagcctggt
    17641 gggttatctg caagagaaag agaagcaggc tacagaagcg gcgatgcaac tggcgcagga
    17701 ttatccggcg gtatcgccta cggagttggg caaagagaaa tttccttttc tctcgtttct
    17761 ttcccagtcg tcatcaaact atttgaccca ttctccctct ctgctgtttt ccaggaaagg
    17821 gagtgaagag aaacaaaatt aatccgtcga ggattgcgct tttttctgcg ctttaatttc
    17881 ctggcggcgc atgcgaaaga agtcactgag caacgccgcg cactcatccg ccagtattcc
    17941 ttccgtaatt tccactcggt gattcatacc cggatgatgc agcacatcca ttaaagatcc
    18001 cgcagcgcca gttttcgcgt cacgcgcacc aaagaccacg cgaccaatgc gactgtggat
    18061 catcgctccg gcacacatta cacatggttc aagcgtgaca tacaacgtgg cgtcgatcag
    18121 acgataattt tgcatcacca gaccaccctg ccgcagggcc atgatttctg catgtgcggt
    18181 gggatcatgg cgaccaatcg ggcggttcca accttcgccg attacccgat tgttatgcac
    18241 taataccgcg ccgaccggca cttcccgctc atcccaggca cgtttcgcca gcgtcagcgc
    18301 gtgacgcatc cagtattcgt ggctaaattc gacttcagac aaaaagaaaa ctccggttat
    18361 aaaagcgcgg cgcattatac acggacgcta tgctttactc tattccagtt gctggagttc
    18421 accgcgcggg gttactcgcc aacgatgctg acagaaataa agcagcggat tgtcctgatt
    18481 actgtcgcta tagccactgt acagccgcag cggagtgccg attttgcgct ccagttgtgc
    18541 gaccttttca tgtcccagac aacgcatcgt caatacccaa ccaccatagc cacgctgaat
    18601 ttggctggcg ataagattaa cccgcggcag ccagggctta tcgaaataaa ccgcttcaac
    18661 cagcggctgc ggagagccgg taatcaacca gatatcagca tcggaactta acaggtaggt
    18721 ggttaatcgc tcctgaacca gcggaaaggc ggtaacattg tcgcgaaacc agcgcacgaa
    18781 atcggcctgc aacgtctgta aacgtgcttc gctgtgacca aaagtgcacc cccacagaag
    18841 cagactcatc ggccagcgtg ccgcacgacc ttttatcaat aacgcaatgg ctataatcgg
    18901 taacaacggc aggacaagta acgcattcag cggttggcga cgtagtaaat agcgcagaaa
    18961 actgccgaac atatcctgct gatgcaatgt tccatctaag tcaaaaaaca ccacacgacg
    19021 ctcgtgagtt gccaaaccat actcctctgg actgaaacat cctgattaat tttcgctact
    19081 acatagccta acagatagat catcactttt ccggcaacaa tcgaccgcat catggctaac
    19141 tggaattttt aattcatggc aattagcggc aatggaatat aaaattcact cgcgtgtgtc
    19201 tcatattgcc tgttgtcgcc actatttcat cgcgaggaaa gatatgaacg gcttacttcg
    19261 tatccgtcag cgttaccagg ggcttgccca gagtgataaa aaactggcgg attatctgct
    19321 gctacaacct gatacggcgc gccatttaag ctctcagcaa ctggccaacg aagccggagt
    19381 cagtcagtcc agcgtcgtga agttcgcgca aaaactcggc tataaaggtt ttccggcgct
    19441 taagttggcg ttgagtgaag cgctggcaag ccagccggaa tcgccctccg tgcccattca
    19501 taaccaaatc cgcggtgatg atccgttacg gctggtcggc gaaaaactga ttaaagaaaa
    19561 tactgccgct atgtacgcaa cgctaaacgt taatagtgaa gagaaactgc atgaatgcgt
    19621 aacaatgttg cgctctgcgc ggcggataat tctgaccggt attggcgctt cgggtctggt
    19681 ggcgcaaaac tttgcctgga agctgatgaa gattggcttc aatgctgccg cagtgcgcga
    19741 tatgcatgcg ctactcgcaa cagtacaggc gtcgtcccct gacgatctgt tattagccat
    19801 ttcctacacc ggtgtacgac gcgagttaaa cctggcggca gatgagatgc tgcgagtggg
    19861 cggaaaagtg ctggcgatta ccggctttac tccgaatgcc ctgcaacagc gtgcttctca
    19921 ttgcctgtat accattgccg aagaacaagc gacaaacagt gcttcaatct ctgcttgtca
    19981 cgctcaggga atgttaacgg atttgctgtt cattgcgctg attcagcagg atctggaact
    20041 ggcaccagaa cgtattcgtc atagtgaagc gctggtgaaa aaactggtct gagtaaagaa
    20101 tgcgcgtata atgcccgccc ggtttgtgtt gttttgagag tttccttatg gcgttgttaa
    20161 tcactaaaaa atgcatcaat tgtgatatgt gtgaacccga atgcccgaat gaggcgattt
    20221 caatgggaga tcatatctac gagattaaca gcgataagtg taccgaatgc gtagggcact
    20281 acgagacacc aacctgccag aaggtgtgcc cgatccccaa tactattgtg aaagatccgg
    20341 cgcatgtcga gacagaagaa cagttgtggg ataaatttgt gctgatgcac cacgcggata
    20401 aaatttaata ttctactctg gaagtagagt attaattata ttactgggaa gccttaacgc
    20461 cattatattt atttaattga tgacattagc ataatcattc actaagttaa tttatatagt
    20521 atctgccaag acacttattt atagttatta aaggcgcgtc cgattggttc accggacgca
    20581 ccttaagtac gtttccttgt gttataagaa cagaaggatc agctgtaaaa cagcaatgat
    20641 gattttgatg acccgtttaa tcaggtatcg gcaatcagtc attcgttttt ccttaaacaa
    20701 ggaatgcagc cgttcagatt agcccttaca tctccccaaa actgaacgtg cgagttattg
    20761 agggtgcatg ctgcactcca caccagagct ttgacgacac cactcgtttc aatgggggaa
    20821 ttctgtggca tggtgtaaag cacagcaaaa tcttcaataa cgaagccaat tttaatgtac
    20881 ttacgaattg gcggtcacgt taatctttcc atcaatattg ctttcttcgt aaaggctcga
    20941 gtttttatgc taaagattgc aagttgcttg taaaagataa gtacactgat ccataatcgc
    21001 tgttgttgag ggtgcatgct gcacaaaatt aaagttaaaa agtaaaaccc ccgttcctta
    21061 ccagttcggg ggttttactt tttaaagaga acggtattat tttaactttc aataattacc
    21121 gtggcacaag catagtggcg ttcatctgcc agcgttacat gcatatttac aacgccgagc
    21181 ttttccgcca gttttaatgc ctcaccccat agccgtagcc gtggtttgcc gagctcatca
    21241 ttgaatactt caaattgatt aaacgccaga ccattgcgga tcccggtgcc aaacgctttt
    21301 gctgcggctt ctttcacagc aaaacgcttc gccagaaaac gcaccggctg gtggtgcgtt
    21361 ttccagattg cccattcgtt atcgcttaat acgcggcgtg ctaggcgctc accggatcgg
    21421 gcgatcactg cttctatgcg agcgatctct acgatatccg tgcctaaacc taatattgcc
    21481 attagccacg cgcttccagc atcagacgct tcatttctgc caccgcatct ttcagtccgg
    21541 tcatcactgc acggccaata atggcatgac cgatattcag ttcatgcatc tcagggatgg
    21601 cggcaatggc tttcacgttg tgataggtca gaccgtgtcc ggcgttaact ttcagaccga
    21661 gactagcggc aaaggtcgcg gctttggcga tacgcaccag ctcttgcgcc tgttcggcgt
    21721 cagttttggc atcagcatag caaccggtgt ggatctcgat aaacggtgcg ccaacctctg
    21781 ccgcagcttt gatctgctct tcatcggcgt cgataaagag agaaacctga atcccggcat
    21841 ctgtcagacg tttgcaggca tcgcgcattt tttcacgctg ccctgcaacg tccaggccgc
    21901 cttcggttgt tacttcctga cgcttttccg gtaccaggca gcaaaaatgt ggcttcgtct
    21961 caacggcgat cgccagcatc tcttcggtca ctgccatctc cagattcatt cgggtatcca
    22021 gcgtctgccg caggatgcgc acatcgcggt cagtaatgtg gcggcgatct tcacgtaaat
    22081 gcacggtaat gccgtccgct cccgcctgct cggcaataaa cgccgcctgc accggatccg
    22141 ggtaagcagt accgcgtgcg ttacgcagcg ttgcaatatg gtcaatgttg acgcctaaca
    22201 gtaattcagc catgacaatc ctcatcattc ataatgtgtt ttcaccgttc gcttaggcat
    22261 aaactgccgg aacagttccc tgctctttaa tggtttaccg ccaagatacg gcttaagcgc
    22321 catgcgggta aagcgtttcg cggcgcgtag tgtgtctgcg tcaggaaatt cccgcgcgtt
    22381 taacgctttt aactgccttc cggtgaacgt tttattgtcg ataacgacgc ttgcgataaa
    22441 ccctttttct tcgcgataac gatacgtcat ggtgtcatct accggctcgc cgctacccgc
    22501 gcaataggta aaattgacgc catagcccag atgcccgagc aatgccagtt caaagcggcg
    22561 cagcgcgggt tctggcgtac cagtggcacc tgcaagagac tgaatgcagt gcaaataatc
    22621 gaaaaagagt tcagagaagc gcgtctcgta ttccagtacg cgggagagaa gttcgttgat
    22681 gtacagaccg ctgtaaagcg tgataccgct taatggcagc gccagcgaga cagcttcagc
    22741 actgcgcagc gttttgactt cgccacgccc gccgaaacgt agcaagagag gggtgaaagg
    22801 ctgtaatgca cctttcagag tagagcgttt agagcgtgcg cctttggcaa ccagacgcac
    22861 gcgacccgat tcctccgtga agacgtccag catcaggctg gtttcgctcc acgggcgact
    22921 atgcaggaca aatgcgcgct gccagccttc catcggagtg actcttaaag atcgtcaacg
    22981 taaccgagac tgcgcagtgc gcgttcgtcg tcggcccaac cggatttcac ttttacccac
    23041 agttcaaggt ggacaggcgc ttcgaacatt tcctgcatgt ctttacgcgc ttcaatcccg
    23101 atggttttga tcttggcccc tttgttgcca ataaccatct tcttctgccc ttcacgctca
    23161 acgagaatca aaccattgat gtcataacca ccgcgttcgt tagagacgaa acgttcgatc
    23221 tccacggtca cggagtacgg cagttcagcg ccgaggaaac gcatcagttt ttcgcggatg
    23281 atttcagacg ccataaaacg ctgtgagcga tcggtgatgt aatcttccgg gaagtgatgg
    23341 gtcgcttcag gcagatgctt acgcacgatt gctgcgatag tgtcgacatt cagcccggtt
    23401 tcagcagaga tcggcacgat atcgaggaag ttcatctgac ttgccaggaa ctgtaagtgc
    23461 ggcagcagat cggctttttc ctgcacgtta tccactttgt tcaccgcgag gatcaccggc
    23521 gctttgccat cgcgcagttt gttgagcacc atttcgtcgt ccggcgtcca gcgggtgcct
    23581 tcaacgacaa aaatcaccag ctcaacgtcg ccaatagagc tgctcgccgc tttgttcatc
    23641 aggcggttaa tagcgcgttt ttcttccata tgcaggccag gggtatcaac gtagatcgcc
    23701 tgatacgcgc cttcagtatg gatccccaca atgcggtgac gagttgtttg cgctttgcgg
    23761 gaggtgatgg agatcttctg ccccagcagt ttgttcaaca atgtggattt gccaacgttc
    23821 ggacgtccga cgatggcaat aaatccgcag taacttttat cgatgctcat tccagctcca
    23881 gttttttcaa cgcctgttcg gcggcagcct gctcagcctt acgacggctt gaacctgtgc
    23941 caaccaccgg ttcactcagg ccgctgacct ggcagtggat agtaaattcc tgatcgtgcg
    24001 cttcgccacg tacctggact accagataag tcggcagcgg cagatggcga ccctgcaaat
    24061 attcttgcaa gcgcgttttc ggatcttttt gtttatcgcc tgggctaatt tcgtccaaac
    24121 gagtttgata ccagttgagg attaatttct cgacggtttg aatatcactg tcgaggaata
    24181 cgccaccaat taatgcttcg acggtatcgg cgagaattga ctcacgacga aatccaccgc
    24241 ttttaagttc accaggccct aaacgtaagc actcgcctaa ctcaaattcg cgcgccagtt
    24301 ccgccagcgt attgccacgg accagcgtgg cgcgcatccg gctcatatcg ccttcatcca
    24361 cacgagggaa acggtgataa agcgcattgg cgataacgta gctcagaata gagtcgccta
    24421 aaaattctaa acgctcgtta tgtttgctgc tggcactacg atgagttaat gcctgctgca
    24481 acagttcctg atgattaaaa gtgtagccca gcttccgttg aagccgatta attacgatgg
    24541 ggttcatgcg ataccaataa ataaatgcgt caacaattca gcacacgaaa cagacctgat
    24601 atacatggct ctgctaactg cttcgctgca gtttcagttt accggtatat ggggaccaac
    24661 gctgtttcgt gtgccgtggc aacctggagg tgccaacctt aaacttcggg ggaatattct
    24721 atacacaacg acgggggatg tcgttagcca cgggagattt atctcataaa taattcacgt
    24781 tgtcgccata acggcgacaa cgtgaacgaa gatgggctat taatggatgc cgccaatgcg
    24841 acttaagcgc acacctgtcg gccattcgcc ttcttgctta tcgaaactca tccagatagc
    24901 cgttgcccga ccgaccagat tcgcttcagg cacaaaaccc cagtaacggc tgtccgcgct
    24961 gttgtcgcgg ttgtcgccca tcatgaagta ttgtcccggc ggaacaatcc aggttgccag
    25021 ttgttgccct ggctgctggt aatacatccc cacctggtcc tgcgcaatcg gcactgtcag
    25081 aattcggtgc gtcacatcac ccagtgtctc tttacgctcg gaaagacgaa ttccattttc
    25141 tttggtttcg tttttcggca cttcaaagaa tccgctggtc gcttccccac cattacggcg
    25201 tgagaaggtc tgaacgaaat cgctcggttc cacgttggag taggtgaccg gtagcgcgtt
    25261 ttcacacgcc tggccggaac tgcatcccgg ttgaatcgtc agctcttttg agaccggatc
    25321 gtaagtgact ttatcgcccg gtaaacccac cgcgcgcttg atgtaatcaa gctttggatc
    25381 ttccggatat ttaaagacca cgatatcgcc gcgtttcgga tgaccggttt cgatcagcgt
    25441 tttctggtag ataggatctt taatgccata agcaaacttc tctaccagaa taaaatcacc
    25501 gattaacaga gtcggcatca tcgaacctga cgggatctgg aacggttcat aaataaacga
    25561 acgtacaatc aatacgatag ccagcaccgg aaaaacagaa gcaccggttt ccagccagcc
    25621 aggcttcggc gcaacctttt tcagggttgc tttatccagt gagtcaccgg cagccgcctg
    25681 cgccgctgcc tgacgttccc gccgtttagg tgcgaaaaag aatttatcca cgcaccataa
    25741 aatgcccgtc accagtgtgg caatcaccag aatcagggca aacatattcg ccatgccaac
    25801 tcctaagggt tatttgttgt ctttgccgac gtgcagaatg gcgaggaacg cttcctgcgg
    25861 cagctcgacg ttaccgatct gcttcatgcg tttcttacct tctttctgct tctgcagcag
    25921 ctttttctta cggctgatat cgccgccata acatttagcc agtacgtttt tacgcagctg
    25981 tttcacggtg gatcgcgcaa taatgtgcgt accaatcgct gcctgaatgg cgatatcaaa
    26041 ctgctggcgt gggatcagat ctttcatctt ctccaccaac tcgcgaccgc ggttttgcga
    26101 attatcacgg tgggtgatca gcgccaacgc atcaacacgt tcaccgttga ttaatacgtc
    26161 tacacgtacc atgtcggacg cctggaagcg tttgaagttg taatccagag acgcataacc
    26221 acgcgaggta gatttcaggc gatcgaagaa gtcgagcacc acttccgcca tcgggatctc
    26281 gtacgtcagc gccacctgat taccgtggta aaccatattg gtctgcacgc cgcgtttttc
    26341 tacgcacagc gtaataacgt tgccgagata tgcctgcggc agcagcatgt gacactctgc
    26401 aatcggctcg cgcagttcgt aaatgttatt taccgcaggc agcttggatg ggctgtcgac
    26461 gtagataact tcccttgacg tggtttcaac ttcatacact acggtcggcg cagtggtgat
    26521 cagatccaga tcgtattcac gttccagacg ttcctggatg atctccatgt gcagcaggcc
    26581 aaggaagccg cagcggaaac caaagcccag cgcgctggag ctttccggct catagaacag
    26641 tgaggcatca ttcaggctga gtttacccag cgcgtcacgg aaggcttcat agtcgtcgga
    26701 acttaccggg aacagaccgg cgtatacctg cggtttgact ttcttaaagc caggcagcgc
    26761 cttttctgcc ggattacgcg ccagcgttaa ggtatcgccg actggagcgc cgtggatatc
    26821 tttaatcgca cataccagcc agcctacttc gccacatttc agttcagtgc ggtcaacctg
    26881 tttcggcgtg aagatgccca gacggtcggc gttataggtc tgcccggtac tcatgacttt
    26941 cactttgtcg cccttacgca gggtgccgtt tttaatacgg ataagtgaaa caacgcccag
    27001 gtagttgtcg aaccatgagt cgataattag tgcctgcaac gggccttccg gatcgccttc
    27061 cggcggcgga atgtcgcgca ccagacgttc gagaacgtcc tgcacgccaa cgccggtttt
    27121 cgctgaacag cgcaccgcgt cagtggcgtc gatgccgacg atatcttcaa tttcttccgc
    27181 cacgcgttca ggatcggctg ccggcaggtc aatcttgttc agtactggca ccacttcgag
    27241 atccatttcc atggcggtgt agcagtttgc cagggtttgc gcttccacgc cctgcccggc
    27301 gtcgaccacc agcaatgcac cttcacaggc agccagcgaa cgggaaactt cataggagaa
    27361 gtctacgtgg cccggggtgt cgataaagtt aagctgatag gtttcgccgt cagacgcttt
    27421 gtagtccagc gtcacgcttt gcgctttgat ggtaatgcca cgctcacgct caagatccat
    27481 ggaatcgaga acctgcgcct ccatttcacg gtcagacagg ccaccgcaga tctggataat
    27541 acggtcagac agcgtcgatt taccgtggtc aatgtgagct atgatcgaaa agttacgtat
    27601 attcttcata aagtatgatt attgtgcctt aatgcccggt taaccaggct tttagaagtc
    27661 gctgttctga gcttaacgtc tgtattaata gaaacgccgc attctacact acaacattga
    27721 ggcgaggaaa tgttcatacc gtatggattg tggtatctgg aaacgtcctc gcatttgtta
    27781 tgcaaaatgc aacaaagcca gtgaaatcac tggctcgcgt cttccgaaga tgtttcaaat
    27841 cgcacaaggc caggcggcaa tgccacgctt aagatgatcg gttgccattc tgcccgggca
    27901 gcaaacttac gcgagtagcc gcgcgcaatc aggaacccgc caataccgcc gaggaccgca
    27961 ccacataatg ctgcgacgtc agaagcaaag agtagctgaa atagcgaagc gatgaggaat
    28021 aatcccacca gcggcgacat ataaaccagt aatgcggagc taagcaggct gccttcggcg
    28081 atccccaatt ccactttttg ccccggcact aacggttcat cacagggtac gacaatggta
    28141 tgcgtggttt gcgggccaag tttattcaac acgcggctac cgcaaccggc gcgtgaagcg
    28201 cagctgctgc atgaagcttt aacatcacaa ctgaccagcg cctgcccgtt ttgccaggag
    28261 acgacggtag cccactcttt gatcattgcg ctgccccgaa cttaatattc tcggcaatgc
    28321 gtttcgccgt ttgcggcggc agttcaccga caatggtgat ttcggcgtta tcacgtacgc
    28381 ttgtactgac ggttctgcgt ccggtgcgca acatctgatc ggtgctcgat ggcgtagcgc
    28441 ggttaacgtt taccgagaag ctgaataatc cgtcagaata gagacgtgat tcgataggca
    28501 tgttgtccat cgtcggtagc ggacgtcgac tactggaaac ttcgctaaaa ccctgtggca
    28561 accaggttgg cgtccagctg aatttagctt tttcacctac aggaacagaa agcaacggcg
    28621 gcaaatttgc cttcgccagc gtctgcatac tgctgctgat atcctgattg acgttaaaag
    28681 caatcacgcg aaattgttcc agcgtttcac catcgcgatc aaggagatca acccgcatcg
    28741 gtaatttcga ttcggtgtcc atccacacga tgtagctgta gcgtgtacca tctcgggcaa
    28801 ccacgcgaat gacttcgcaa agacgatcag caatacgcgt gcgcccgacg gagataaagt
    28861 cgtagtaagg agaaaggcgt ttgaaatcgg tatagatgag cgatggcaga gaatcaacaa
    28921 tgtaatcgcc attaagcgtg aacggttcaa gtcccggttc aaaatagctg atttcatcgc
    28981 cgcgctgtac cacttcccgg cgcgggccat ccatttgcaa caattgtgca agaggacggt
    29041 tatcgaggcg tgcatgtcga taacgcagag actcaacacc ctgtttattg atgctgatga
    29101 atgacagctc gtaattcagt gactgactgg ccaggttcat ctgctgtaat aacgccccgg
    29161 acgcgggagt ggccgaggcg ttagcagaga ataacaggct acctgtcact aatgacatgg
    29221 caaaccaaag ttgcttcatt actgcgattg cgttcctaaa gtttgaattc ctggcacctg
    29281 tacagcggct tgctgggttt gcgcctgctc aaactgaagc tgttcagagt ggagtcggcg
    29341 ttgcagttcg taatcctgca acattgcatt aatgcgacga cgctgctcct gtacctgctg
    29401 ctgttgacca ttgtttgcgg tcgcttcaga aggtactccc aggcttaccg ggctggcttt
    29461 acccatcatc ggcagtgtat taaataccgg cgtttcgggc tgctgggacg tttcagattg
    29521 tccattatag tgctggacgc caacgataac tgcaagcgat acgcatgctg ctacgcccat
    29581 ttgggtaagc tgtgccgccc acggacgtac tttctgccag aatggcattt tctgccattg
    29641 atgcggcgca ggctgggctt ccgggatcaa tgtcgccggt tgacgtactg gctcttcttc
    29701 aatggcggcc atcacgcgtg aagagatatc gaaatggagc acctcgggag tatcaccccg
    29761 cattgagtca cggattaagt gatagctttc ccaggttttc tgcatttctg ggttatgagc
    29821 cagttcgtta agcagctcac tatccagcgt ttcgccatcc attaaagcgg aaagttgttc
    29881 tttctgcatg cctaataccc ttatccagta tcccgctatc gtcaacgcct gataagcggt
    29941 tgaactttgt tatcaatagc ttccctcgct cggaagatac gtgaacgcac cgtacctacc
    30001 ggacaatcca tgatagcggc tatctcttca tagctcaggc catccagctc ccgcaaggtt
    30061 attgccatgc gcaaatcttc cgggagggac tcaatagttc ggaaaactat ctgtctcagt
    30121 tcttctgaca acattaagtt ctcagggttc gaaatttctt tcaacgcgcc gccactttcg
    30181 aagttttcag cttcaatggc atccacatca ctggaaggtg gacgacgccc ctgagcaacc
    30241 aggtaatttt tcgctgtatt tacagcaatc cgatacagcc atgtataaaa agcgctatct
    30301 ccccggaacg aatccagcgc acgataggct ttaataaaag cttcttgtac cacatcggga
    30361 acatcacccg acggcacata gcgggaaacc agactcgcca ctttatgctg atagcgcact
    30421 accagtaagt taaaggcttt ctgatctccc ttctggaccc gttcaaccag gacctggtcc
    30481 gttaactgct cgctcatccg aggtaaagtc tccccaaacc aaatttccac gcgctatcga
    30541 aacgccactc cattagctgc aatttgagca agcaaagggt tagagtgtct cgtttttgta
    30601 aagttccgta acgcatctgt ttttgtttgt catgctgtag acggatcatt atctatcatt
    30661 ataagtctac agaatctgaa catcgcatta tctgtgtaga aatgcccatt taactgcctg
    30721 aagagtaacc caacggcctt tttatttcac cacctaatcc tccaccagcc agtaacttct
    30781 ctttttctcg ccgccctgca tcagcgtgtt tagcaactgt aacaaatatt aaaatagcag
    30841 gtgtttattc gcacaacatg atgctatact gaccaaaccg tgtttagtaa attaaacaaa
    30901 gaaaatgaat actctccctg aacattcatg tgacgtgttg attattggta gcggcgcagc
    30961 cggactttca ctggcgctac gcctggctga ccagcatcag gtcatcgttc taagtaaagg
    31021 cccggtaacg gaaggttcaa cattttatgc ccagggcggt attgccgccg tgtttgatga
    31081 aactgacagc attgactcgc atgtggaaga cacattgatt gccggggctg gtatttgcga
    31141 tcgccatgca gttgaatttg tcgccagcaa tgctcgttcc tgtgtgcaat ggttaattga
    31201 ccagggggtt ttgtttgata cccacgttca accgaatggc gaagaaagct accatctgac
    31261 ccgtgaagga ggacatagtc accgtcgtat tcttcatgcc gccgacgcca ccggtagaga
    31321 agtacaatcc acgctggtga gcaaggcgca gaaccatccg aatattcgtg tgctggagcg
    31381 cagcaacgca gttgatctga ttgtctctga caaaattggc ctgccgggca cgcgacg
//
LOCUS       DAJTMC010000030        30944 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.030, whole genome shotgun sequence.
ACCESSION   DAJTMC010000030 DAJTMC010000000
VERSION     DAJTMC010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30944
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.030"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            248..631
                     /gene="secE"
                     /locus_tag="OO130_001219"
     CDS             248..631
                     /gene="secE"
                     /locus_tag="OO130_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HCR5364709.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            633..1178
                     /gene="nusG"
                     /locus_tag="OO130_001220"
     CDS             633..1178
                     /gene="nusG"
                     /locus_tag="OO130_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HCR5364710.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1338..1766
                     /gene="rplK"
                     /locus_tag="OO130_001221"
     CDS             1338..1766
                     /gene="rplK"
                     /locus_tag="OO130_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HCR5364711.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGAGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1770..2474
                     /gene="rplA"
                     /locus_tag="OO130_001222"
     CDS             1770..2474
                     /gene="rplA"
                     /locus_tag="OO130_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HCR5364712.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            2887..3384
                     /gene="rplJ"
                     /locus_tag="OO130_001223"
     CDS             2887..3384
                     /gene="rplJ"
                     /locus_tag="OO130_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HCR5364713.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3451..3816
                     /gene="rplL"
                     /locus_tag="OO130_001224"
     CDS             3451..3816
                     /gene="rplL"
                     /locus_tag="OO130_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HCR5364714.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4136..8164
                     /gene="rpoB"
                     /locus_tag="OO130_001225"
     CDS             4136..8164
                     /gene="rpoB"
                     /locus_tag="OO130_001225"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HCR5364715.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8241..12464
                     /gene="rpoC"
                     /locus_tag="OO130_001226"
     CDS             8241..12464
                     /gene="rpoC"
                     /locus_tag="OO130_001226"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HCR5364716.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            12677..13216
                     /locus_tag="OO130_001227"
     CDS             12677..13216
                     /locus_tag="OO130_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-shock protein C"
                     /protein_id="HCR5364717.1"
                     /translation="MKQEVEKWRPFGHPDGDIRDLSFLDAHQAVYVQHHEGKEPLEYR
                     FWVTYSLHCFTKDYEHQTNEEKQSLMYHAPKESRPFCQHRYNLARTHLKRTILALPES
                     NVIHAGYGSYAVIEVDLDGGDKAFYFVAFRAFREKKKLRLHVTSAYPISEKQKGKSVK
                     FFTIAYNLLRNKQLPQPSK"
     gene            complement(13626..14759)
                     /gene="thiH"
                     /locus_tag="OO130_001228"
     CDS             complement(13626..14759)
                     /gene="thiH"
                     /locus_tag="OO130_001228"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HCR5364718.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINGKTAVDVERALNASQFTRDDMMA
                     LLSPAASGYLEQLAQRAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAKTEYAELKQLGLDGVMVYQETYHEATYARHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(14756..15526)
                     /gene="thiG"
                     /locus_tag="OO130_001229"
     CDS             complement(14756..15526)
                     /gene="thiG"
                     /locus_tag="OO130_001229"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HCR5364719.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAEMLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSHFAHATSPLTGFLEASA"
     gene            complement(15528..15728)
                     /gene="thiS"
                     /locus_tag="OO130_001230"
     CDS             complement(15528..15728)
                     /gene="thiS"
                     /locus_tag="OO130_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HCR5364720.1"
                     /translation="MQILFNDQPMQCATGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(15712..16467)
                     /gene="thiF"
                     /locus_tag="OO130_001231"
                     /pseudo
     CDS             complement(15712..16467)
                     /gene="thiF"
                     /locus_tag="OO130_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709786.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
     gene            complement(16460..17095)
                     /gene="thiE"
                     /locus_tag="OO130_001232"
     CDS             complement(16460..17095)
                     /gene="thiE"
                     /locus_tag="OO130_001232"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HCR5364721.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVISAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(17095..18990)
                     /gene="thiC"
                     /locus_tag="OO130_001233"
     CDS             complement(17095..18990)
                     /gene="thiC"
                     /locus_tag="OO130_001233"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HCR5364722.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYLTGTHSGVRV
                     PMREIQLSPTLIGGSKEQPQFEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTKARLADDGLDELRFSGVLTPKRAKAGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGARLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAATQTIEMGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(19071..19171)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(19224..19700)
                     /gene="rsd"
                     /locus_tag="OO130_001234"
     CDS             complement(19224..19700)
                     /gene="rsd"
                     /locus_tag="OO130_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HCR5364723.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQKVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            19795..20568
                     /gene="nudC"
                     /locus_tag="OO130_001235"
     CDS             19795..20568
                     /gene="nudC"
                     /locus_tag="OO130_001235"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HCR5364724.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLVQQQRRHDMGSVRQVIDLDVVLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            20608..21672
                     /gene="hemE"
                     /locus_tag="OO130_001236"
     CDS             20608..21672
                     /gene="hemE"
                     /locus_tag="OO130_001236"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HCR5364725.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKVTRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            21682..22353
                     /gene="nfi"
                     /locus_tag="OO130_001237"
     CDS             21682..22353
                     /gene="nfi"
                     /locus_tag="OO130_001237"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HCR5364726.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLMVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            22396..22986
                     /locus_tag="OO130_001238"
     CDS             22396..22986
                     /locus_tag="OO130_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HCR5364727.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVVLSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQVGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGESNIGIIFQQ"
     gene            23173..23445
                     /gene="hupA"
                     /locus_tag="OO130_001239"
     CDS             23173..23445
                     /gene="hupA"
                     /locus_tag="OO130_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HCR5364728.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            23458..24153
                     /locus_tag="OO130_001240"
     CDS             23458..24153
                     /locus_tag="OO130_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HCR5364729.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALLLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(24155..24580)
                     /gene="zraP"
                     /locus_tag="OO130_001241"
     CDS             complement(24155..24580)
                     /gene="zraP"
                     /locus_tag="OO130_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HCR5364730.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            24818..26194
                     /gene="zraS"
                     /locus_tag="OO130_001242"
     CDS             24818..26194
                     /gene="zraS"
                     /locus_tag="OO130_001242"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HCR5364731.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIVQHGVISVTVSESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            26191..27515
                     /gene="zraR"
                     /locus_tag="OO130_001243"
                     /pseudo
     CDS             26191..27515
                     /gene="zraR"
                     /locus_tag="OO130_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
     gene            complement(27512..28801)
                     /gene="purD"
                     /locus_tag="OO130_001244"
     CDS             complement(27512..28801)
                     /gene="purD"
                     /locus_tag="OO130_001244"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HCR5364732.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDDVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACESKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLLLEEVAGGKVFHAGTKLADDEQVVTNGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28813..30402)
                     /gene="purH"
                     /locus_tag="OO130_001245"
     CDS             complement(28813..30402)
                     /gene="purH"
                     /locus_tag="OO130_001245"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HCR5364733.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7337 a         7987 c         8433 g         7187 t
ORIGIN      
        1 gcaaaagttc tgagctaatt gccgataaca tttgactcaa tgcgcactaa aagggcatca
       61 tttgatgccc tttttgcacg ctttcgtacc agaacctggc tcatcagtga ttttctttgt
      121 cataatcatt gctgagacag gctctgttga gggcgtataa tccgaaaagc taatacgcgt
      181 ttcgatttgg tttgcctcgc gatcgcgggg tgaaaatgtt tgtagaaaac ttctgacagg
      241 ttggtttatg agtgcgaata ccgaagctca aggaagcggg cgcggcctgg aagcgatgaa
      301 gtgggtcgtt gtggtggcat tgctcttggt ggcgattgtc ggcaactacc tttatcgcga
      361 cattatgctg ccgctgcgtg cgctggccgt agtaattctg attgctgcag cgggtggtgt
      421 cgcgctgtta acgacaaaag gtaaagctac cgttgctttt gcccgtgaag cgcgtaccga
      481 agtccgtaag gtcatttggc cgactcgcca ggaaacattg cacaccacgc tgattgtggc
      541 tgcggttacc gcagtaatgt cactgatcct gtggggactg gatggtattc tggttcgcct
      601 ggtatccttt atcactggcc tgaggttctg agatgtctga agctcctaaa aagcgctggt
      661 acgtcgttca ggcgttttcc ggttttgaag gccgcgtagc aacgtcgctg cgtgagcata
      721 tcaaattaca caacatggaa gatttgtttg gtgaagtcat ggtaccaacc gaagaagtgg
      781 ttgaaatccg tggcggtcag cgtcgcaaaa gcgaacgtaa attcttccct ggctacgtcc
      841 tcgttcagat ggtgatgaac gacgcgagct ggcacctggt gcgcagcgta ccgcgagtga
      901 tgggcttcat cggcggtact tccgatcgtc ctgcgccaat cagcgataaa gaagtcgatg
      961 cgattatgaa ccgcctgcag caggttggtg ataagccgcg tccgaaaacg ctgtttgaac
     1021 cgggtgaaat ggtccgtgtt aatgatggtc cgttcgctga cttcaacggt gttgttgaag
     1081 aagtggatta cgagaaatct cgtctgaaag tgtctgtttc tatcttcggt cgtgcgaccc
     1141 cggtagagct ggacttcagc caggttgaaa aagcctaacc cagcgatcaa aaaagcggcg
     1201 atttaatcgt tgcacaaggc gcgagattgg aatacaattt cgcgcctttt atttttatgg
     1261 gccttgcccg taaaaacgat tttttatatc acggggagcc tctcagaggc gttattaccc
     1321 aacttgagga atttataatg gctaagaaag tacaagccta tgtcaagctg caggttgcag
     1381 ctggtatggc taacccgagt ccgccagtag gtccggctct gggtcagcag ggcgtaaaca
     1441 tcatggaatt ctgcaaagcg ttcaacgcaa aaactgattc catcgaaaaa ggtctgccga
     1501 ttccggtagt aatcaccgtt tacgctgacc gttctttcac tttcgttacc aagaccccgc
     1561 cggcagcagt tctgctgaaa aaagcggctg gtatcaagtc tggtgccggt aagccgaaca
     1621 aagacaaagt gggtaaaatt tcccgcgctc agctgcagga aatcgcgcag accaaagctg
     1681 ccgacatgac tggtgccgac attgaagcga tgactcgctc catcgaaggt actgcacgtt
     1741 ccatgggcct ggtagtggag gactaagaaa tggctaaact gaccaagcgc atgcgtgtta
     1801 tccgcgagaa agttgatgca accaaacagt acgacatcaa cgaagctatc gcactgctga
     1861 aagagctggc gactgctaaa ttcgtagaaa gcgtggacgt agctgttaac ctcggcatcg
     1921 acgctcgtaa atctgaccag aacgtacgtg gtgcaactgt actgccgcac ggtactggcc
     1981 gttccgttcg cgtagccgta tttacccaag gtgcaaacgc tgaagctgct aaagctgcag
     2041 gcgcagaact ggtaggtatg gaagatctgg ctgaccagat caagaaaggc gaaatgaact
     2101 ttgacgttgt tattgcttct ccggatgcaa tgcgcgttgt tggccagctg ggccaggttc
     2161 tgggtccgcg cggcctgatg ccaaacccga aagtgggtac tgtaacaccg aacgttgctg
     2221 aagcggttaa aaacgctaaa gctggccagg ttcgttaccg taacgacaaa aacggcatca
     2281 tccacaccac catcggtaaa gtggactttg acgctgacaa actgaaagaa aacctggaag
     2341 ctctgctggt tgcgctgaaa aaagcaaaac cgactcaggc gaaaggcgtg tacatcaaga
     2401 aagttagcat ctccaccacc atgggtgcag gtgttgcagt tgaccaggct ggcctgagcg
     2461 cttctgtaaa ctaatgcctt tacgtgggcg gtgattttgt ctacaatctt acccccacgt
     2521 ataatgctta atgcagacgt atatccgaga tattcgggtt gtggcaaggc ggcaactgag
     2581 tgagtcgcca agagcatagc taactatgtg actggtgcga atgaaggaag ccaacgccgc
     2641 cacaagttga atagcgacgg atagaaaaga tttgttcgtt ggagcttggc ctatccaggc
     2701 ctccgtcgaa gaccgcagga gtttcgcaag aaacttaatc ccctgcgtag acggtgacag
     2761 aacgctaaga ttattctttt atattctggc ttgtttctgc tcaccgtaat taagacgctc
     2821 tctccgtttg gaggagtgaa gtgagttcca gagattttct ctggcaaaca tccaggagca
     2881 aagctaatgg ctttaaatct tcaagacaaa caagcgattg ttgctgaagt cagcgaagta
     2941 gccaaaggcg cgctgtctgc agtagttgcg gattcccgtg gcgtaactgt agataaaatg
     3001 actgaactgc gtaaagcagg tcgcgaagct ggcgtataca tgcgtgttgt tcgtaacacc
     3061 ctgctgcgcc gtgctgttga aggtactccg ttcgagtgcc tgaaagacgc gtttgttggt
     3121 ccgaccctga ttgcatactc tatggaacac ccgggcgctg ctgctcgtct gttcaaagag
     3181 ttcgcgaaag cgaatgcaaa atttgaggtc aaagccgctg cctttgaagg tgagctgatc
     3241 ccggcgtctc agatcgaccg cctggcaact ctgccgacct acgaagaagc aattgcacgc
     3301 ctgatggcaa ccatgaaaga agcttcggct ggcaaactgg ttcgtactct ggctgctgta
     3361 cgcgatgcga aagaagctgc ttaatcgcag ttatcttttt aacgcattcg cttacgtata
     3421 aacttattct gatattcagg aacaatttaa atgtctatca ctaaagatca aatcattgaa
     3481 gcagttgcag ctatgtctgt aatggacgtt gtagaactga tctctgcaat ggaagaaaaa
     3541 ttcggtgttt ccgctgctgc tgctgtagct gtagctgctg gcccggttga agctgctgaa
     3601 gaaaaaactg aattcgacgt aattctgaaa gctgctggcg ctaacaaagt tgctgttatc
     3661 aaagcagtac gtggcgcaac tggcctgggt ctgaaagaag ctaaagacct ggtagaatct
     3721 gcaccggctg ctctgaaaga aggcgtgagc aaagacgacg cagaagcact gaaaaaagct
     3781 ctggaagaag ctggcgctga agttgaagtt aaataagcca acccttccgg ttgcagcctg
     3841 agaaatcagg ctgatggctg gtgacttttt agtcaccagc ctttttgcgc tgtaaggcgc
     3901 cagtagcgtt tcacactgtt tgactactgc tgtgcctttc aatgcttgtt tctatcgacg
     3961 acttaatata ctgcgacagg acgtccgttc tgtgtaaatc gcaatgaaat ggtttaagcg
     4021 tgatagcaac aggcattgcg gaaagtgttc cattttccgg tcaacaaaat agtgttgcac
     4081 aaactgtccg ctcaatggac agatgggtcg acttgtcagc gagctgagga accctatggt
     4141 ttactcctat accgagaaaa aacgtattcg taaggatttt ggtaaacgtc cacaagttct
     4201 ggatgtacct tatctccttt ctatccagct tgactcgttt cagaaattta tcgagcaaga
     4261 tcctgaaggg cagtatggtc tggaagcagc tttccgttcc gtattcccga ttcagagcta
     4321 cagcggtaat tccgagctgc aatacgtcag ctaccgcctt ggcgaaccgg tgtttgacgt
     4381 ccaggaatgt caaatccgtg gcgtgaccta ttccgcaccg ctgcgcgtta aactgcgtct
     4441 ggtgatctat gagcgcgaag cgccggaagg caccgtaaaa gacattaaag aacaagaagt
     4501 ctacatgggc gaaattccgc tcatgacaga caacggtacc tttgttatca acggtactga
     4561 gcgtgttatc gtttcccagc tgcaccgtag tccgggcgtc ttctttgact ccgacaaagg
     4621 taaaacccac tcttcgggta aagtgctgta taacgcgcgc atcatccctt accgtggttc
     4681 ctggctggac ttcgaattcg atccgaagga caacctgttc gtacgtatcg accgtcgccg
     4741 taaactgcct gcgaccatca ttctgcgcgc cctgaactac accacagagc agatcctcga
     4801 cctgttcttt gaaaaagtta tctttgaaat ccgtgataac aagctgcaga tggaactggt
     4861 gccggaacgc ctgcgtggtg aaaccgcatc ttttgacatc gaagctaacg gtaaagtgta
     4921 cgtagaaaaa ggccgccgta tcactgcgcg ccacattcgc cagctggaaa aagacgacgt
     4981 caaacttatc gaagtcccgg ttgagtacat cgcaggtaaa gtggttgcta aagactatat
     5041 tgatgagtct accggcgagc tgatctgcgc agcgaacatg gagctgagcc tggatctgct
     5101 ggctaagctg agccagtctg gtcacaagcg tatcgaaacg ctgttcacca acgatctgga
     5161 tcacggccca tatatctctg aaaccttacg tgtcgaccca actaacgacc gtctgagcgc
     5221 actggtagaa atctaccgca tgatgcgccc tggcgagccg ccgactcgtg aagcagctga
     5281 aagcctgttc gagaacctgt tcttctccga agaccgttat gacttgtctg cggttggtcg
     5341 tatgaagttc aaccgttctc tgctgcgcga agaaatcgaa ggttccggta tcctgagcaa
     5401 agacgacatc attgatgtta tgaaaaagct catcgatatc cgtaacggta aaggcgaagt
     5461 cgatgatatc gaccacctcg gcaaccgtcg tatccgttcc gttggcgaaa tggcggaaaa
     5521 ccagttccgc gttggcttgg tacgtgtaga gcgtgcggtg aaagagcgtc tgtctctggg
     5581 cgatctggat accctgatgc ctcaggatat gatcaacgcc aagccgattt ccgcagcagt
     5641 gaaagagttc ttcggttcca gccagctgtc tcagtttatg gaccagaaca acccgctgtc
     5701 tgagattacg cacaaacgtc gtatctccgc actcggccca ggcggtctga cccgtgaacg
     5761 tgcaggcttc gaagttcgag acgtacaccc gactcactac ggtcgcgtat gtccaatcga
     5821 aacccctgaa ggtccgaaca tcggtctgat caactctctg tccgtgtacg cacagactaa
     5881 cgaatacggc ttccttgaga ctccgtatcg taaagtgacc gacggtgttg taactgacga
     5941 aattcactac ctgtctgcta tcgaagaagg caactacgtt atcgcccagg cgaactccaa
     6001 cctggatgaa gaaggccact tcgtagaaga cctggtaact tgccgtagca aaggcgaatc
     6061 cagcttgttc agccgcgacc aggttgacta catggacgta tccacccagc aggtggtatc
     6121 cgtcggtgcg tccctgatcc cgttcctgga acacgatgac gccaaccgtg cattgatggg
     6181 tgcgaacatg caacgtcagg ccgttccgac tctgcgcgct gataagccgc tggttggtac
     6241 tggtatggaa cgtgctgttg ccgttgactc cggtgtaact gcggtagcta aacgtggtgg
     6301 tgtcgttcag tacgtggatg cttcccgtat cgttatcaaa gttaacgaag acgagatgta
     6361 tccgggtgaa gcaggtatcg acatctacaa cctgaccaaa tacacccgtt ctaaccagaa
     6421 cacctgtatc aaccagatgc cgtgtgtgtc tctgggtgaa ccggttgaac gtggcgacgt
     6481 gctggcagac ggtccgtcca ccgacctcgg tgaactggcg cttggtcaga acatgcgcgt
     6541 agcgttcatg ccgtggaatg gttacaactt cgaagactcc atcctcgtat ccgagcgtgt
     6601 tgttcaggaa gaccgtttca ccaccatcca cattcaggaa ctggcgtgtg tgtcccgtga
     6661 caccaagctg gggccggaag agatcaccgc tgacatcccg aacgtgggtg aagctgcgct
     6721 ctccaaacta gatgaatccg gtatcgttta cattggtgcg gaagtgaccg gtggcgacat
     6781 tctggttggt aaggtaacgc cgaaaggtga aactcagctg accccagaag aaaaactgct
     6841 gcgtgcgatc ttcggtgaga aagcctctga cgttaaagac tcttctctgc gcgtaccaaa
     6901 cggtgtatcc ggtacggtta tcgacgttca ggtctttact cgcgatggcg tagaaaaaga
     6961 caaacgtgcg ctggaaatcg aagaaatgca gctcaaacag gcgaagaaag acctgtctga
     7021 agaactgcag atcctcgaag cgggtctgtt cagccgtatc cgtgctgtgc tggtagccgg
     7081 tggcgttgaa gctgagaagc tcgacaaact gccgcgcgat cgctggctgg agctgggcct
     7141 gacagacgaa gagaaacaaa atcagctgga acagctggct gagcagtatg acgaactgaa
     7201 acacgagttc gagaagaaac tcgaagcgaa acgccgcaaa atcacccagg gcgacgatct
     7261 ggcaccgggc gtgctgaaga ttgttaaggt atatctggcg gttaaacgcc gtatccagcc
     7321 tggtgacaag atggcaggtc gtcacggtaa caagggtgta atttctaaga tcaacccgat
     7381 cgaagatatg ccttacgatg aaaacggtac gccggtagac atcgtactga acccgctggg
     7441 cgtaccgtct cgtatgaaca tcggtcagat cctcgaaacc cacctgggta tggctgcgaa
     7501 aggtatcggc gacaagatca acgccatgct gaaacagcag caagaagtcg cgaaactgcg
     7561 cgaattcatc cagcgtgcgt acgatctggg cgctgacgtt cgtcagaaag ttgacctgag
     7621 taccttcagc gatgaagaag ttatgcgtct ggctgaaaac ctgcgcaaag gtatgccaat
     7681 cgcaacgccg gtgttcgacg gtgcgaaaga agcagaaatt aaagagctgc tgaaacttgg
     7741 cgacctgccg acttccggtc agatccgcct gtacgatggt cgcactggtg aacagttcga
     7801 gcgtccggta accgttggtt acatgtacat gctgaaactg aaccacctgg tcgacgacaa
     7861 gatgcacgcg cgttccaccg gttcttacag cctggttact cagcagccgc tgggtggtaa
     7921 ggcacagttc ggtggtcagc gtttcgggga gatggaagtg tgggcgctgg aagcatacgg
     7981 cgcagcatac accctgcagg aaatgctcac cgttaagtct gatgacgtga acggtcgtac
     8041 caagatgtat aaaaacatcg tggacggcaa ccatcagatg gagccgggca tgccagaatc
     8101 cttcaacgta ttgttgaaag agattcgttc gctgggtatc aacatcgaac tggaagacga
     8161 gtaattctcg ctcaaacagg tcactgctgt cgggttaaaa cccggcagcg gattgtgcta
     8221 actccgacgg gagcaaatcc gtgaaagatt tattaaagtt tctgaaagcg cagactaaaa
     8281 ccgaagagtt tgatgcgatc aaaattgctc tggcttcgcc agacatgatc cgttcatggt
     8341 ctttcggtga agttaaaaag ccggaaacca tcaactaccg tacgttcaaa ccagaacgtg
     8401 acggcctttt ctgcgcccgt atctttgggc cggtaaaaga ttacgagtgc ctgtgcggta
     8461 agtacaagcg cctgaaacac cgtggcgtca tctgtgagaa gtgcggcgtt gaagtgaccc
     8521 agactaaagt acgccgtgag cgtatgggcc acatcgaact ggcttccccg actgcgcaca
     8581 tctggttcct gaaatcgctg ccgtcccgta tcggtctgct gctcgatatg ccgctgcgcg
     8641 atatcgaacg cgtactgtac tttgaatcct atgtggttat cgaaggcggt atgaccaacc
     8701 tggaacgtca gcagatcctg actgaagagc agtatctgga cgcgctggaa gagttcggtg
     8761 acgaattcga cgcgaagatg ggtgcggaag caatccaggc tctgctgaag agcatggatc
     8821 tggagcaaga gtgcgaacag ctgcgcgaag agctgaacga aaccaactcc gaaaccaagc
     8881 gtaaaaagct gaccaagcgt atcaaactgc tggaagcgtt cgttcagtct ggtaacaaac
     8941 cagagtggat gatcctgacc gttctgccgg tactgccgcc agatctgcgt ccgctggttc
     9001 cgctggatgg tggtcgtttc gcgacttctg acctgaacga tctgtatcgt cgcgtcatta
     9061 accgtaacaa ccgtctgaaa cgtctgctgg atctggctgc gccggacatc atcgtacgta
     9121 acgaaaaacg tatgctgcag gaagcggtag acgccctgct ggataacggt cgtcgcggtc
     9181 gtgcgatcac cggttctaac aagcgtcctc tgaaatcttt ggccgacatg atcaaaggta
     9241 aacagggtcg tttccgtcag aacctgctcg gtaagcgtgt tgactactcc ggtcgttctg
     9301 taatcaccgt aggtccatac ctgcgtctgc atcagtgcgg tctgccgaag aaaatggcgc
     9361 tggagctgtt caaaccgttc atctacggca agctggaact gcgtggtctt gctaccacca
     9421 ttaaagctgc gaagaaaatg gttgagcgcg aagaagctgt cgtttgggat atcctggacg
     9481 aagttatccg cgaacacccg gtactgctga accgtgcacc gactctgcac cgtctgggta
     9541 tccaggcatt tgaaccggta ctgatcgaag gtaaagctat ccagctgcac ccgctggttt
     9601 gtgcggcata taacgccgac ttcgatggtg accagatggc tgttcacgta ccgctgacgc
     9661 tggaagccca gctggaagcg cgtgcgctga tgatgtctac caacaacatc ctgtccccgg
     9721 cgaacggcga accaatcatc gttccgtctc aggacgttgt tctgggtctg tactatatga
     9781 cccgtgactg tgttaacgcc aaaggcgaag gcatggtgct gactggcccg aaagaagcag
     9841 aacgtctgta tcgctctggt ctggcttctc tgcatgcgcg cgttaaagtg cgtatcaccg
     9901 agtatgaaaa agatgcaaac ggtgaattag tagcgaaaac cagcctgaaa gacacgactg
     9961 ttggccgtgc cattctgtgg atgattgtac cgaaaggtct gccttactcc atcgtcaacc
    10021 aggcgctggg taaaaaagca atctccaaaa tgctgaacac ctgctaccgc attctcggtc
    10081 tgaaaccgac cgttattttt gcggaccaga tcatgtacac cggcttcgcc tatgcagcgc
    10141 gttctggtgc atctgttggc atcgatgaca tggtcatccc ggagaagaaa cacgaaatca
    10201 tctccgaggc agaagcagaa gttgctgaaa ttcaggagca gttccagtct ggtctggtaa
    10261 ctgcgggcga acgctacaac aaagttatcg atatctgggc tgcggcgaac gatcgtgtat
    10321 ccaaagcgat gatggataac ctgcaaactg aaaccgtgat taaccgtgac ggtcaggaag
    10381 agaagcaggt ttccttcaac agcatctaca tgatggccga ctccggtgcg cgtggttctg
    10441 cggcacagat tcgtcagctt gctggtatgc gtggtctgat ggcgaagccg gatggctcca
    10501 tcatcgaaac gccaatcacc gcgaacttcc gtgaaggtct gaacgtactc cagtacttca
    10561 tctccaccca cggtgctcgt aaaggtctgg cggataccgc actgaaaact gcaaactccg
    10621 gttacctgac tcgtcgtctg gttgacgtgg cgcaggacct ggtggttacc gaagacgatt
    10681 gtggtaccca tgaaggtatc atgatgactc cggttatcga aggtggtgat gttaaagagc
    10741 cgctgcgcga tcgcgtactg ggtcgtgtaa ctgctgaaga cgttctgaag ccgggtactg
    10801 ctgatatcct cgttccgcgc aacacgctgc tgcacgaaca gtggtgtgac ctgctggaag
    10861 agaactctgt cgacgcggtt aaagtacgtt ctgttgtatc ttgtgacacc gactttggtg
    10921 tatgtgcgca ctgctacggt cgtgacctgg cgcgtggcca catcatcaac aagggtgaag
    10981 caatcggtgt tatcgcggca cagtccatcg gtgaaccggg tacacagctg accatgcgta
    11041 cgttccacat cggtggtgcg gcatctcgtg cggctgctga atccagcatc caggtgaaaa
    11101 acaaaggtag catcaagctc agcaacgtga agtcggttgt gaactccagc ggtaaactgg
    11161 ttatcacttc ccgtaatact gaactgaaac tgatcgacga attcggtcgt accaaagaaa
    11221 gctacaaagt accttacggt gcggtactgg cgaaaggcga tggcgaacag gttgctggcg
    11281 gcgaaaccgt tgcaaactgg gacccgcaca ccatgccggt tatcaccgaa gtaagcggtt
    11341 ttgtacgctt tactgacatg atcgacggtc agaccattac tcgtcagaca gacgaattga
    11401 ccggtctgtc ttcgctggtg gttctggatt ccgcagaacg taccgcaggt ggtaaagatc
    11461 tgcgtccggc actgaaaatc gttgatgctc agggtaacga cgttctgatt ccgggtaccg
    11521 atatgcctgc gcagtacttc ctgccgggta aagcgattgt tcagctggaa gatggcgtac
    11581 agatcagctc tggtgacacc ctggcgcgta ttccgcagga atccggcggt accaaggaca
    11641 tcaccggtgg tctgccgcgc gttgcggacc tgttcgaagc acgtcgtccg aaagagccgg
    11701 caatcctggc tgaaatcagc ggtatcgttt ccttcggtaa agaaaccaaa ggtaaacgtc
    11761 gtcttgttat caccccggta gacggtagcg atccgtacga agagatgatt ccgaaatggc
    11821 gtcagctcaa cgtgttcgaa ggtgaacgtg tagaacgtgg tgacgtaatt tccgacggtc
    11881 cggaagcgcc gcacgacatt ctgcgtctgc gtggtgttca tgctgttact cgttacatcg
    11941 ttaacgaagt acaggacgta taccgtctgc agggcgttaa gattaacgat aaacacatcg
    12001 aagttatcgt tcgtcagatg ctgcgtaaag ctaccatcgt taacgcgggc agctccgact
    12061 tcctggaagg cgaacaggtt gaatactctc gcgtcaagat cgcaaaccgc gaactggaag
    12121 cgaacggcaa agtgggtgca acttactccc gcgatctgct gggtatcacc aaagcgtctc
    12181 tggcaaccga gtccttcatc tccgcggcat cgttccagga gaccactcgc gtgctgaccg
    12241 aagcagccgt tgcgggcaaa cgcgacgaac tgcgcggcct gaaagagaac gttatcgtgg
    12301 gtcgtctgat cccggcaggt accggttacg cgtaccacca ggatcgtatg cgtcgccgtg
    12361 ctgcgggtga agctccggct gcaccgcagg tgactgcaga agacgcatct gccagcctgg
    12421 cagaactgct gaacgcaggt ctgggcggtt ctgataacga gtaatcgtta atccgcaaat
    12481 aacgtaaaaa cccgcttcgg cgggtttttt tatgggggga gtttagggaa agagcatttg
    12541 tcagaatatt taaggaattt ctgaatactc ataatcaatg tagagatgac taatatcctg
    12601 aaactgactg aactaattga gtcaaactcg gcaaggattc gatactattc ctgtgtaact
    12661 ttcttaagga acgagaatga aacaggaagt ggaaaagtgg cgaccttttg gacatccgga
    12721 tggtgatatt cgtgatttat catttcttga tgctcatcag gctgtctacg ttcagcatca
    12781 tgagggcaaa gagcctttag agtatcgctt ttgggttacc tactctcttc actgcttcac
    12841 aaaagattat gaacatcaga cgaacgaaga aaaacaatcg ttaatgtacc acgcgcctaa
    12901 agaatctcgt cccttctgcc agcaccgtta taacttagcg cgcacacact taaaaagaac
    12961 tattttggcg ctgccagaaa gcaacgttat tcatgccggg tatggtagct atgccgtgat
    13021 tgaggtggac ttagacggag gagataaggc attttacttt gttgcgttca gggctttcag
    13081 ggaaaagaaa aaactccgtt tgcatgtaac tagcgcttat cccatttctg aaaaacagaa
    13141 aggtaaatca gtgaaatttt tcaccattgc ctacaactta ttgagaaata agcagcttcc
    13201 tcagccctca aaataacaaa acccacctta aggtgggttt cgccagagaa ttatctctgg
    13261 tattcagaac gccattaccg gactttgcct tgaccttgcg ataatcgcag gttgcgggat
    13321 gtctgaattt cttcagtctg ctgcatcctg gaagatgaga acatgtgttc ttattttcgt
    13381 ctctatcata gttgagtatt tactctctta caatcagatc tctttcattg ctcaacaggc
    13441 gatggcttca gactttgcat tacggaattt ttaagaaagg cagggcgaaa cgaggaagaa
    13501 gcttttcgcc ctggacaaca ttcctgctga cggcactacc ataaaaaaac acaacgcttt
    13561 caacaacctt gcgtttttca tcttccgtag cggctcggaa taaatttacg ttcttacatt
    13621 ccgtctcata gtctttgcga ggcccgtccc agatagctgt cccagtcttt ccataccggc
    13681 tgcaaaccct gagcggttaa cgcggcagca accgcttccg gtctgcgatc gtcgtgcggt
    13741 gagaactgtt ccaactcggg gtgattatcg gcatagccac ctggctgcgt tttcgagaag
    13801 gcgctgacgt tattgatcgc cagcggaata acgcgatcgc gaaaccacgg tgattcccgc
    13861 gtggagagtg acagttcaat ctccggtgca agcagtcgga aggcgcagat ggtttgcact
    13921 aactggcgtt catccataat cgacgcaggc tcaatgccgc cagtacacgg gcgcaggcgc
    13981 ggaaaggaga cagagtaacg gctttgccag taatgctgtt gcagccatag caaatgttcg
    14041 gcaaccatat agcagtcaac gcgccagttg tcggaaaggc caattagcgc gccgaggcct
    14101 atcttatcaa tccccgcacg ccccagccga tccggcgttt ccagccgcca gaagaagtcc
    14161 tgttttttgc ctttcagatg atggcgggca taagtcgcct cgtgatatgt ctcctgataa
    14221 accatcacgc catccagacc aagctgcttt aactcggcgt attccgtctt cgccagcggt
    14281 tgcacttcca tttgtagtga agagaactgt tcacgcaggg cggggagatg acgacgaaag
    14341 taatccatcc ccactttcgc ctgatgttca ccagtgacta atagcagatg ttcaaagccc
    14401 atctcccgta tagcggcgct ttccctggca atatccgctt catccagcgt tttgcgcttg
    14461 atgcgattac tcatggaaaa tccgcagtac gtgcagtcgt tagcgcaaag attggaaaga
    14521 taaagcggga cgtagaaact aactgtgttg ccaaatcgct gacgggtcag acgctgcgcc
    14581 cgttgggcca gttgttccag atagccactg gcggctggcg ataacagcgc catcatatcg
    14641 tcgcgggtga attgcgaggc atttagcgcc cgctctacgt caacagccgt tttgccgttg
    14701 atacgcaggc ggatgtcatc ccagtccagt tgtcgccagc gatcgctgaa ggttttcatg
    14761 ccgatgcctc cagaaatcca gtcagcgggc tggtggcatg agcaaaatga ctgcggctgc
    14821 ccggtccgga ctgacgtgcc agcaggcctg cttctaccgc cagacgaaat gccttcgcca
    14881 tgttgacggg atcgtccgct acggcaatcg ccgtattcac taacaccgcg tcggccccca
    14941 tttccagggc ctgcgctgca tggctgggaa cgccgatgcc agcatcgaca accaccggaa
    15001 ctgtggcctg ctggataata atctccagca tggcacgggt ttccagtccc tgattcgagc
    15061 caatcggcgc gccgagcggc atcaccgctg cacagccgac ttcttccaga cgtttacaca
    15121 ataccggatc ggccccgcag taaggcagca cgacaaatcc ctgttgtacc agcatttcgg
    15181 cggctttcag agtttcgatg ggatcgggca acagccagcg ggcgtcaggg tgaatctcta
    15241 attttaacca gtttgtgcct aacgcttcac gtgccagatg ggcggcgaaa atggcttctt
    15301 ccgctgtttt cgccccggaa gtatttggca gcagggtcac acccgccgcg ataagcggtt
    15361 cgaggatagc gtcgttgtgc tggcgcaagt cgacacgttt catcgccagt gtcaccagct
    15421 ggctgccgga agcgcggatc gcctccacca tcagttgtga agaagcgaat ttccctgtgc
    15481 cggtaaacag atgtgaatca aacgttttgt ccgcaatacg taacatctca accccctgca
    15541 ataacctgaa aaagcaggat ctggtcgcca tcctgcacga tatgttgcgt ccactgctca
    15601 cgcgggacga tttgctgatt aatcgccaga gccgcgcccg cttgtcgttg gtcgagttgc
    15661 tccagtagtt cgtgaacagt ttgcccggtg gcgcactgca tcggttgatc gttaaacagg
    15721 atctgcattg cttcctccgc ataccgggca accactggcg cggcgcaacg ccaggctgcg
    15781 ccactggctc gatttaccgt cgaacagtcg gagttctccc gcaggtgtct ctataccgct
    15841 taataactta atggcttcca gtgcctgcaa agtgcccata accccgacca ccgggccaac
    15901 cacgcccgcc gtgcggcagt tgcgttctgg ctcctggtta tctggccaca ggcagcggta
    15961 acacccctgc tcccagggcg gcgtcagtac catcaactga ccgccaaatc cgaccgcgct
    16021 ggcggtgata agcggcgtgt tgagtaccac gcaggcggca ttaatctcct ggcgagtcgc
    16081 catattgtcg gtacagtcga gcaccacatc ggcccgtgca accgcatctt ttaacgcctc
    16141 acccgttaac cgttgttgta atgctgtcag ttgaatgtcg ggattcaact gtgtcagtcg
    16201 ctgttggctg acctgcgatt tcgggctgtt gatatcttca gtggtaaaga gaatttgtcg
    16261 ttacagattg cttaaatgca catcgtcgtc atctgccagt accagcgtcc cgacgccagc
    16321 gcccgccagg tatagcgcag caggtgtacc cagcccgcca agaccgataa tcagcacctg
    16381 gctgtcgagc agtttttgct gcccgtcaag agcgatatca tcgagcagga tttggcggct
    16441 ataacgcata aaatcacggt cattcatcgc caactcctgc aatttccagc aactgtgccg
    16501 ttgccaaacg ccagtctgcg gcttgagtaa tggcgctgat gacggcgata ctgccgacac
    16561 ccgttgctat caccgcaggc gcgcgtgcca gactgatacc gccaatcgcc acggtgggat
    16621 aatccgccag tcgctcaaca tgccgtgcca gctgttccag cccctgcggt gcagaaggca
    16681 tctgtttggt ttgcgtcggg aacacatgtc ccagcgcgat ataagagggg cgtgctgcca
    16741 gcgcgacgtc gatttccata tcgtcatgtg tcgaaacacc cagccgcagg cctgccgcgc
    16801 ggatggcatt gagatcggtg gcttgcaaat cttcctgccc caaatggacg ccatacgcct
    16861 gatgcttgat cgcgagccgc cagtaatcgt tgataaacaa tcgcgcgtta tagcggcggc
    16921 ccagcgcaat tgccgccacg acatcggctt ccacctcttc atcgcgccga tctttgatgc
    16981 gtagctggag agtacgtacg cctgcatcca acagacgttc gatccactgt acgctgtcca
    17041 ccactgggta cagtcctaaa cgaaaaggta caggaggaaa atcaggctga tacatcacgc
    17101 ttcctcctta cgcaggtaga tttctccgcc tctggcacgg aagttctccg acatatccgc
    17161 cattcccatt tcaatagttt gcgtggcggc gtaatcacgc acttcctggc tgattttcat
    17221 cgagcagaat ttcggcccac acatggagca aaaatgggcg actttacctg actcttgcgg
    17281 cagggtttca tcgtgataag cgcgggcggt aaacgggtcg agggccagat taaactggtc
    17341 ttcccagcga aattcgaagc gggctttcga catggcgtta tcgcgaattt gcgcgcccgg
    17401 atgccctttc gccaggtcag cggcgtgggc ggcaatctta taggtgataa gcccctgctt
    17461 aacatcttct ttattaggca gacccagatg ctcttttggt gttacgtaac agagcatcgc
    17521 gcagccaaac cagccaatca tcgccgcacc aatccccgac gtgaagtggt catagcccgg
    17581 cgcaatatcg gtagttagcg gccccagagt gtaaaacggc gcttcgtggc agtgctctaa
    17641 ctcctcggtc atattgcggc ggatcatctg catcggcacg tggcctgggc cttcaatcat
    17701 cacctgcacg tcatattccc aggcaatttt ggtcagttcg cccagcgtat gcagctcggc
    17761 aaactgcgct tcatcgttgg cgtcctgaat agaaccgggg cgcagaccgt cgcccagcga
    17821 cagcgaaacg tcataagcgg cacagatttc acaaatttcg cggaagtgtt gatagaggaa
    17881 attttcctga tgatgggaga ggcaccattt cgccataatc gaaccgccgc gagagacgat
    17941 accggtcagg cgtttcgcgg tcatcggcac atagcgcaac agtacgcccg catggatagt
    18001 gaagtaatcc acaccttgct cggcctgttc cagcagcgtg tcgcggaacg cttcccaggt
    18061 aagatcttcg gcgatcccgt taaccttctc cagcgcctgg tagatcggca ctgtaccgat
    18121 cggcaccgga ctgttacgca aaatccactc gcgggtttcg tgaatatagc gaccggtgga
    18181 gagatccatc accgtatccg ctccccagcg cgtggaccat accagctttt ccacttcttc
    18241 ttcgatggaa gaggtgaccg ccgagttgcc gatattggcg ttaactttta ccaggaagtt
    18301 acggccaata atcatcggct ccgattccgg atgattaatg ttggccggga taatcgcacg
    18361 tccggcagca acttcatcac ggacaaattc cgcagtgata ttttccggca gacgtgcgcc
    18421 aaagctcatt cccggatgct ggtggcgtaa aacttcgcta cgtatgcgct cgcggcccat
    18481 attctcgcgg atggcgatga attccatttc cggtgtgatg atgccctggc gggcgtagtg
    18541 cagttgggtg acacggcgtc ctgctttggc gcgttttggt gttagtacgc cgctaaaacg
    18601 cagttcgtcg aggccatcat ctgccagccg cgctttagtg taatcggaac tgcgcacggt
    18661 aagttcttcg gtatcgccgc gcgcatcgat ccacggctgg cgtagttttg ccagcccttg
    18721 ctgcacgtta atggcgatct gcggatcgcc atatgggccg gaggtgtcgt agaccggaat
    18781 cgcttcgttt tcttcaaact gcggctgttc tttgctgccg ccgattagcg tcgggctaag
    18841 ctggatctca cgcatcggca cgcgaacgcc agagtgtgtg ccagtgaggt aaatgcgttt
    18901 tgagttggga aaggcggtgc cttccagggt gtcgataaaa tgttgggccc gggcgcgttg
    18961 ttcgcggcgg gtcagttttg ttgcagacat agctcattcc aaaaagttaa ggacgtggct
    19021 tgtcagacga cggatgaagc aagagacgat cgccgcaaag gcgatgcgat agcagattaa
    19081 ctcttgttcc cttcgcaggt attagcctga tcaggttccg cggatcccga ataaacggtc
    19141 tcagccagtt aaggcactcc gacaagaaat tagcccccac aaacggggcg ttgaatgtaa
    19201 attacgcgtt aacagcgcag aactcaagca ggatgtttga cgcgggcggc gtcaagcacc
    19261 agcagaatca gcttatcttc cagcacaaag cgcgcttcca gcgcctcgcc gatgtcagat
    19321 aaaacctttt gaaactcaag gtaattatca tgatcgatgg cggtttccag gctggaatcg
    19381 tagtaatcca taatctgttg ggtattggct tcgagttgcg gccaaatctt cgcggcgcgc
    19441 gcgagttgcc cgttgccttc cagcttatga agaatgcgct cataaatact gaaatgtccg
    19501 gcagacaagt aatcgaccag gctctgacaa aaatcatcaa gggctttttc gtttagcctc
    19561 atgtacgatt ctttgccagg cttaatgcca accagattgt agtaagccac gagcagatgc
    19621 ttacgtacat gtagccagcg atcaaccagt ttgttacttc ctctgacgcg ctccgtcagg
    19681 ttatcgagct ggttaagcat gattgactcc gcaagtttgt attcaaaaac tgctcagtga
    19741 gaaatgtaaa aaccatgtta aacatgccag tgatgcaaag gtagtgcaag agctatggat
    19801 cgtataattg aaaaattaga tcacggctgg tgggtcgtca gccatgaaca aaaattatgg
    19861 ttgccgaagg gagaattgcc atatggcgaa gcggcaaatt tcgatcttgt gggtcagcgc
    19921 gcactacaaa tcggcgaatg gcagggggaa cctgtttggt tagtacaaca gcagcggcgt
    19981 cacgatatgg ggtcggtacg tcaggtcatt gatctcgatg ttgtgctgtt tcaactggcc
    20041 ggacgaggcg tacaactggc ggagttttac cgatcgcata aatactgtgg ttactgcggg
    20101 catgaaatgt atccgagcaa aaccgaatgg gcgatgctgt gcagccattg ccgtgagcgt
    20161 tactacccgc aaatcgcccc ctgcattatt gttgccatcc gtcgcgatga ttcgatcctc
    20221 ctcgcccagc atacccgcca tcgtaacggt gtccatacag tacttgccgg attcgtcgaa
    20281 gtgggcgaaa ccctcgagca ggcagtcgcg cgggaagtga tggaagagag cggaattaaa
    20341 gttaaaaact tgcgttacgt gacttctcag ccgtggccgt ttcctcagtc tttaatgacc
    20401 gcgtttatgg cggaatatga cagcggcgac atcgtgatcg acccgaaaga attgctcgag
    20461 gcgaactggt atcgctatga cgatttgccg ttactcccgc cgcccggcac cgtagcgcgc
    20521 cgtctgatag aagatactgt ggcgatgtgt cgggcagagt atgagtgatg atacactgac
    20581 cgcctgacgc actaaggaac agccaaaatg accgaactta aaaacgatcg ttatctgcgg
    20641 gcgctgctgc gccagcccgt tgatgtcact ccagtatgga tgatgcgcca ggcgggtcgc
    20701 tatctaccgg aatataaagt cacgcgcgcc caggcgggcg attttatgtc gctgtgcaaa
    20761 aacgccgagc tggcgtgcga agtgactttg caaccgctgc gtcgctaccc gctggatgcg
    20821 gcgatcctct tttccgatat cctcaccgtg ccggacgcga tggggttagg gctctatttt
    20881 gaagccggag aaggtccgcg ttttacctcg ccagtcacct gcaaagccga cgtcgataag
    20941 ctgccaattc cggacccgga agatgagctg ggttacgtga tgaacgcggt gcgtaccatt
    21001 cgtcgcgaac tgaaaggcga agtgccgctg attggttttt ccggcagccc gtggacgctg
    21061 gcaacctaca tggtggaagg cggcagcagc aaagccttca ccgtgatcaa aaaaatgatg
    21121 tatgccgatc cgcaggcgct gcacgctcta ctcgataaac tggcgaaaag cgtcactttg
    21181 tatctgaatg cgcagattaa agccggtgct caggcagtga tgattttcga cacctggggc
    21241 ggtgtgctta ccgggcgcga ttatcaacag ttctcgctct attacatgca taaaattgtt
    21301 gatggtttac tgcgtgaaaa cgacggtcgc cgcgtaccgg tcacgctgtt taccaaaggc
    21361 ggcggacagt ggctggaagc gatggcagaa accggttgtg atgcgttggg cctcgactgg
    21421 acaacggata tcgccgatgc gcgccgccgt gtgggcaata aagtcgcgtt gcagggtaat
    21481 atggatccgt cgatgctgta cgcgccgcct gcccgcattg aagaagaagt agcgactata
    21541 cttgcaggtt tcggtcacgg cgaaggtcat gtctttaacc ttggtcacgg cattcatcag
    21601 gatgtgccgc cagaacatgc tggcgtgttc gtggaggcag tgcatcgact gtctgaacaa
    21661 tatcaccgct aaggagtgat tatggatctc gcgtcattac gtgctcaaca aatcgaactg
    21721 gcttcttctg tgatccgcga ggatcgactc gataaagatc caccggatct gatcgccgga
    21781 gccgatgtcg ggtttgagca gggcggagaa gtgacgcgag cggcgatggt gctgctgaaa
    21841 tatccctcgc ttgagctggt ggagtataaa gttgcccgca tcgccaccac catgccttac
    21901 attccaggtt ttctttcctt ccgcgaatat cctgcgctgc tggcagcgtg ggagatgctg
    21961 tcgcaaaagc cggatttagt gtttgtcgat ggtcatggga tctcgcatcc tcgccgtctt
    22021 ggcgtcgcca gccattttgg tttgatggtg gatgtgccaa cgatcggcgt ggcgaaaaaa
    22081 cggctctgcg gtaaattcga accgctctcc agcgaaccgg gcgcgctggc cccactgatg
    22141 gataaaggcg agcagctggc ctgggtctgg cgcagcaaag cgcgctgtaa cccgttgttt
    22201 atcgctaccg gccatcgggt cagcgtggac agcgcgctgg cgtgggtaca acgctgcatg
    22261 aaaggctatc gtctgccgga gccaacgcgc tgggcggacg cggtggcctc ggaacgtccg
    22321 gcgttcgtgc gctatacagc aaatcagccc taattcaggt aaactgcgga taatttccgt
    22381 atttgagaac ttaacatgtt acaaaaccca attcatctgc gtctggagcg cctggaaagc
    22441 tggcagcatg tcactttcat ggcttgctta tgcgaacgca tgtaccccaa ttacgccatg
    22501 ttctgccagc aaaccggttt tggtgatggg caaatttacc gtcgtattct cgatctcatc
    22561 tgggaaacgc tgaccgttaa agacgcaaaa gtaaatttcg acagccaact ggagaaattt
    22621 gaagaagcga ttccttcagc cgacgatttc gatctgtacg gcgtttatcc ggcaatcgat
    22681 gcctgcgtgg tgttaagtga actggtccat tcgcgtttga gtggtgaaac gctcgaacac
    22741 gcggtggaag tgagtaagac ctccatcacg accgttgcga tgctggaaat gactcaggtt
    22801 ggtcgcgaaa tgagcgatga agagcttaaa gaaaacccag ctgtagagca agaatgggac
    22861 attcagtggg aaatattccg acttttagct gagtgcgaag aacgcgatat cgagctgata
    22921 aaaggcctta gggcagacct gcgtgaggcg ggtgagagca atattggtat aatttttcag
    22981 caataagacc agaaaacgtg atttaacgcc tgatttgtcg tacctggagt cttccctttc
    23041 gccccccgtc tggtctacat ttggggggcg aaaaaaagtg gctatcggtg cgtgtatgca
    23101 ggagagtgct attctggcat ttccgtcgca ctcgatgctt agcaagcgat aaacacattg
    23161 taaggataac ttatgaacaa gactcaactg attgatgtaa ttgcagagaa agcagaactg
    23221 tccaaaaccc aggctaaagc tgctctggag tccactctgg ctgcaattac tgagtctctg
    23281 aaagaaggcg atgctgtaca actggttggt ttcggtacct tcaaagtgaa ccaccgcgct
    23341 gagcgtactg gccgcaaccc gcagaccggt aaagaaatca aaattgccgc agctaacgta
    23401 ccggcatttg tttctggcaa ggcactgaaa gacgcagtta agtaagattg cgtggcagtg
    23461 aacagtttta acgaaggggt ggtttcaccc cttttgtctt tctggcgtcg atcattgatg
    23521 ctggctggcg ctctgcttct cactgcctgt agtcataact cttcacttcc tccctttacc
    23581 gccagtggat ttgctgaaga ccagggcgcg gtacgcatct ggcgaaaaga cagcggcgat
    23641 aatgtgcatc tgcttgccgt gtttagcccg tggcgcagtg gcgataccac gacgcgagag
    23701 tatcgctggc agggcgataa cctcacgctc atcaatatca atgtttacag caaaccgccg
    23761 gtgaatattc gcgcgcgttt tgacgatcgc ggtgatctga gctttatgca acgtgaatcc
    23821 gatggggaaa agcagcagct ttctaacgac caaatcgatt tataccgtta tcgtgctgat
    23881 cagatccgcc agattagcga tgccttacgt caggggagag tcgtgctgcg ccaggggcgc
    23941 tggcatgcga tggaacagac cgtgaccacc tgcgaagggc aaaccattaa acctgattta
    24001 gattcgcagg cgatagcgca tatcgagcgc cgccagagcc gctcttctgt tgatgtcagc
    24061 gtggcatggc tggaagcgcc cgaaggttcg caattactgt tagtggcaaa ctctgatttc
    24121 tgtcgctggc aacccaacga gaaaacgttc tgatttacca gtggcccata cccatatgac
    24181 cgccaccacc gcagccgccg tagcccatgc ccattccggc tccgcgcgga atacccgctt
    24241 cagccatcgc gatatctcgt ttcacccgta actcatctaa cgactgacgc aaattctcca
    24301 tctctttggc gaccgcgtta attttgctgc tatccggtgg gttcgcggct aacagggcat
    24361 tgtattcata acgcttcgtc accagttgct gttgcagtgc gctgctttga gcgtaaaagt
    24421 cattatggat tttctgccac gctgtctgtt gttcgctggt caaaggcgcg gcattttgct
    24481 gccacatacc gtgtccgccg tgagcaaatg cagatgtcga tcccatcgcc attgctgaaa
    24541 gcgccatcat taccagggca attttcgtgt tccgtttcat ggttaatcct ccagtggttg
    24601 tcttacttcg ggtattgcat cttccgtgcc aacgatgaaa cgctgatatg acgggtaatc
    24661 tggcatgata aacgagtaaa aatgactcgc ctgctgcggg tagcgagtca tttttactca
    24721 ttgaaactta agcctttgtg ttacagcgca gggtaagcgc tgataaaaga tggcatgatt
    24781 tctgctgtca gaaagggatg agcaggcaaa gaagaagatg cgttttatgc aacgttctaa
    24841 agactcctta gctaaatggt taagcgcgat cctccccgtg gtcattgttg ggctggtagg
    24901 gttgtttgcg gtgacggtga ttcgcgatta tgggcgcgag actgccgccg ccagacaaac
    24961 gctgctggaa aaaggcagtg tacttattcg cgctcttgaa tccggctcgc gcgtcggcat
    25021 ggggatgcgc atgcatcatg cgcagcagca ggccttactg gaagaaatgg ccgggcagcc
    25081 tggagtacgt tggtttgcgg tcacggatga acaaggaaca atcgtgatgc atagcaactc
    25141 cggcatggtg ggaaaacagc tttattcccc gcaggaaatg cagcagttac atccgggaga
    25201 tgaagaagcg tggcggcgga tcgatagcgc agacggtgag cctgttctgg aaatttatcg
    25261 ccagtttcaa ccgatgtttg ctgctggaat gcaccggatg cgccatatgc agcagtatgc
    25321 cgcgacacca caagcaattt tcatcgcttt cgacgccagt aatattgtga gtgccgaaga
    25381 tcgtgagcag agaaacaccc tgattatcct cttcgccctg gcgacggtct tgctggcaag
    25441 cgtattgtca ttcttctggt atcgccgcta tctgcgctcg cgccagcttc tacaagatga
    25501 aatgaagcgc aaagagaagc tggtggcact ggggcatctg gcggcaggcg ttgcccacga
    25561 aatccgtaat ccactttcct caattaaagg gctggcgaaa tactttgccg aacgcgcgcc
    25621 agcaggggga gaagcgcatc aactggcgca ggtgatggcg aaagaagccg accgtttaaa
    25681 ccgcgtggta agcgagttgc tggaactggt taagccaacg catctggctt tgcaggcggt
    25741 ggatctcaac acgctgatta accactcatt acagctggta agccaggatg caaacagccg
    25801 ggagatccag ttacgcttta ccgccaacga cacattaccg gaaattcagg ccgatccgga
    25861 caggctgact caggtcctgt tgaatctcta tctcaatgct attcaggcga ttgttcagca
    25921 tggcgtgatt agcgtgacgg tcagcgaaag cggcgcgggc gtgaaaatca gcgttaccga
    25981 cagcggtaag ggaattgcgg cagatcagct tgaagccatc ttcactccgt acttcaccac
    26041 caaagccgaa ggcaccggat tggggctggc ggtcgtgcat aatattgttg aacaacacgg
    26101 tggtacaatt caggtcgcaa gccaggaggg aaaaggctca acgttcaccc tctggcttcc
    26161 ggtcaatatt acgcgtaagg acccacaagg atgacgcacg ataatatcga tattctggtg
    26221 gtggatgatg acattagcca ctgcactatt ttgcaggctt tactgcgcgg ctggggctat
    26281 aacgtcgcgc tggcgaacag cgggcgacag gcgctggagc aggtgcggga acaggttttt
    26341 gatcttgtgc tttgcgatgt gcgaatggcg gagatggacg gcatcgccac gctgaaagag
    26401 atcaaagcgt taaacccggc aattccggtg ctgattatga ctgcgtactc cagcgtcgag
    26461 acggcggtag aggcactgaa aactggggcg ctggattatc tcatcaagcc gctggatttc
    26521 gataacctac aggcgacgct ggaaaaggcg ctcgcgcata cgcacagtat tgatgctgaa
    26581 acacctgcgg tgactgccag ccagttcggt atggtcggta aaagcccggc gatgcaacac
    26641 ctgctcagtg aaatcgccct cgtcgcgcca tcggaagcca cagtgctgat ccacggcgat
    26701 tccggcaccg gtaaagagct ggtcgccagg gcgattcacg ccagtagcgc acgtagcgaa
    26761 aaaccgctgg taacgctcaa ctgtgcggca ctcaacgaat ccttgctgga atctgaattg
    26821 ttcggtcacg aaaaaggggc gtttactgga gccgataaac gccgggaagg gcgctttgtt
    26881 gaggcggacg gcggcacgct gtttctcgat gaaattggcg atatctcgcc gatgatgcag
    26941 gtgcgtctgc tgcgtgcgat tcaggagctc gaagttcagc gtgtcggtag caaccagact
    27001 atctcggttg atgtccggct gattgcggcg acccatcgcg atcttgccgc agaggtgaat
    27061 gccgggcgtt ttcgccagga tctctactat cgcctgaatg tggtggcgat tgaagtacca
    27121 tcgctgcggc aacggcggga agatatccct ctgctggctg gccattttct gcagcgcttt
    27181 gccgagcgta atcgcaaggc ggtaaaaggt tttacgcccc aggcgatgga tctgttgatt
    27241 cattacgact ggccgggaaa tattcgtgag ctggaaaacg cggtggaacg ggcagtggtg
    27301 ctgctgccgg ggaatatatt tccgaacgcg agctgccgct ggcgattgcc agtacgccga
    27361 tcccgctggg gcaaagtcag gatattcagc cgctggtgga agtggaaaaa gaggtgattc
    27421 tggcggcact ggagaaaacg ggcggcaaca aaactgaagc cgcccgtcag ttagggatca
    27481 cgcgtaaaac gttattggca aaactgtcgc gttagttctg ctcgcgttcg atagcacgcc
    27541 agccgatatc tttccggcag aagcagtcgt cccagtggat atcggtcatt aaggcatagg
    27601 cgcgtttctg cgcttctgcc acggtatgac ccagcgcggt gacgcacagt acgcgcccgc
    27661 cgttggtcac tacctgctcg tcatccgcca gttttgtgcc cgcgtggaac actttgccgc
    27721 ctgccacttc ttccagcagc aggccgtgga tcacgtcacc ggtgcggtaa tcacccggat
    27781 atccacccgc agccatcacc acgccgagag aagcgcgttc atcccactcg gacgttttct
    27841 cgtccagttt gctttcacag gccgccaggc agagctcaac cagatcggac ttcatgcgca
    27901 gcataatcgg ctgggtttcc ggatcgccaa agcggcagtt aaattcaata accttcggat
    27961 tgccctgttt gtcgatcatc aggcccgcgt agagaaaacc ggtgtaggtg ttgccttccg
    28021 ccgccatgcc tttcacggtt ggccagataa tacgttccat ggtgcgctga tgaacgtcat
    28081 cggttactac cggcgcgggg gagtaagcgc ccatcccgcc ggtgtttggt ccggtatctt
    28141 tatcgcctac gcgtttgtga tcctggctgg tagccatcgg cagcacatgc tcgccgtcca
    28201 ccatcacgat aaagctcgct tcttcgccat cgaggaactc ttcgataacg atgcgatgac
    28261 ccgcgtcgcc aaaagcgttg cccgccagca tatcgtgaac agccgcttcc gcttcttcca
    28321 gcgtcatcgc cacgataacg cctttcccgg cagccagacc gtccgcttta atgacgattg
    28381 gcgcgccttt ctcacgcaga tacgccagcg caggttctac ctcggtgaag ttctggtatt
    28441 ccgccgtagg gatcttatgg cgggccagga aatctttggt aaacgctttt gagccttcca
    28501 gttgggccgc acctgcggtt gggccgaaga ttttcagccc ggcggcgcgg aaggtatcga
    28561 ccacgccttt caccagcggc gcttccgggc cgacgatggt cagatcaatc ttttcgtttt
    28621 gtgcgaaatc caacagcgcc gggatatcgg tcacgccaat agcaacgttt tgcagcgcgg
    28681 gttccagcgc agtgcctgca ttacccggag caacaaaaac agtctcaacc agcggcgact
    28741 gggccgcttt ccaggccagc gcgtgctcgc gcccgccgtt accaatcact aatactttca
    28801 tctattgctc cattaatggc ggaagtggcg catgtcggtg aagagcatcg caataccgtg
    28861 ctcgtcggcg gcggcaatca cttcgtcatc acggatggaa ccgccaggct ggattacgca
    28921 ggtcacgccc gcagcggcgg cggcatcaat accgtcgcgg aacgggaaga acgcgtcaga
    28981 agccatcgag gaacctttca cttccaggcc ttcatcggca gctttaatac cggcgatttt
    29041 cgcggagtac acgcggctca tctggcccgc gccaataccg atggtcatat tgtttttggc
    29101 atagacgata gcattggatt tcacgaactt cgccactttc cagcagaaca gcgcatcgcg
    29161 cagttcctgt tcggtcggct gacgttgggt gacgacgcgc agttcttccg caccgaccat
    29221 ccccaggtca cgatcctgaa ccagcagacc gccgttcacg cgtttgaaat caagacccgg
    29281 aacacgctcg ccccactgac cgcaggtcag aacgcgtacg ttctgcttgg cggcggtgat
    29341 tttcagggct tcttcgctgg cggaaggcgc aataatcact tcgacaaact gacgagaaat
    29401 gatggcctgt gcggtttccg catctagctc gcggttaaag gcaatgatgc cgccgaatgc
    29461 ggaggttggg tcggttttgt acgcgcgatc gtaagcatca agaatggaat tgccgatagc
    29521 tacaccgcaa gggttggcgt gcttcacaat cacacatgcc ggctcggcga actctttcac
    29581 gcactccagc gccgcatcgg tatcggcgat gttgttataa gagagggctt taccctgaac
    29641 ctgggttgcg gtagcaacgg aggcttcttt cacattctct tctatataga aggcagcctg
    29701 ctggtggctg ttctcgccgt aacgcatatc ctgcttctta atgaagttca ggttcagcgt
    29761 gcgtgggaag cgaccggcgg cttctttgct ttcaccgtgg tacgccggaa ccatgctgcc
    29821 gaagtagttg gcaatcatgc tgtcgtaggc ggcagtgtgt tcgaaggctt tgatggcgag
    29881 gtcgaaacgg gttgcaagcg tcagcgatcc ttcgttgtca tccatctctt taataatggc
    29941 gtcgtagtcg ctgctcttca ccacgattgc gacatcttta tggttcttag cggcggagcg
    30001 caccatggtc gggccgccga tatcgatgtt ctcaaccgca tcttccagcg agcagccttc
    30061 acgagcaaca gtctgggcga acggatacag gttaacaacc accatatcga taggctggat
    30121 ctgatgttct tccataatgg catcgtcctg gccgcgacgg cccagaatgc caccatgtac
    30181 tttcggatgc agggtcttca cgcgtccatc catcatctcc gggaaaccgg tgtaatcgga
    30241 aacttcggtt accggcagac ctttttctgc taacagacgg gcagtgcccc ctgtagacag
    30301 cagctccaca ccgcgtgcgg aaagtgcctg ggcgaattcg acgataccgg ctttgtcaga
    30361 aacactgagc agagcgcggc ggactggacg acgttgttgc atggtaaatc ccctggattt
    30421 gactattaca gagagcgtta gctgaatttt tcgcgaaaaa ctcagctaac gcccctaacg
    30481 gggcatcctt atttttcgcc cgcattgtaa cgaaaacgtt tgcgcaacgc tcgcgaattt
    30541 ttctctttca atggtgatca caattttgac agtggctacc gtgggaaaaa tacagaaatt
    30601 acattgatga ttgtggataa ctctgtgcgt aaaaaggtat aaagcggggt tttgctgggg
    30661 aatgcagcag tcagtcattt ttctgcaatt tttctattgc ggcctgcgga gaactcccta
    30721 taatgaacct ccatcgacac ggcggatgtg aatcacttca cacaaacagc cggttcggtt
    30781 gaagagaaaa atcctgaaat tcagggttga ctctgaaaga ggaaagcgta atatacgcca
    30841 cactcacgac agtgcgctaa agctcgtcgc aactgctctt taacaattta tcagacaatc
    30901 tgtgtgggta ctcgaagata cggattctta acgtcgcaag acga
//
LOCUS       DAJTMC010000031        29257 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.031, whole genome shotgun sequence.
ACCESSION   DAJTMC010000031 DAJTMC010000000
VERSION     DAJTMC010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29257
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.031"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            <1..154
                     /locus_tag="OO130_001246"
     CDS             <1..154
                     /locus_tag="OO130_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2135 domain-containing protein"
                     /protein_id="HCR5364734.1"
                     /translation="NYYGGRSETELTTAQLTLITDEGSVNEKQETFIVPMRNAGELTL
                     VKSFDW"
     gene            429..3278
                     /gene="rcsC"
                     /locus_tag="OO130_001247"
     CDS             429..3278
                     /gene="rcsC"
                     /locus_tag="OO130_001247"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HCR5364735.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGGEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGEPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(3478..4128)
                     /gene="rcsB"
                     /locus_tag="OO130_001248"
     CDS             complement(3478..4128)
                     /gene="rcsB"
                     /locus_tag="OO130_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HCR5364736.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(4145..6817)
                     /gene="rcsD"
                     /locus_tag="OO130_001249"
     CDS             complement(4145..6817)
                     /gene="rcsD"
                     /locus_tag="OO130_001249"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HCR5364737.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVNTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTISDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGDNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESLPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQPQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            7556..8686
                     /gene="ompC"
                     /locus_tag="OO130_001250"
     CDS             7556..8686
                     /gene="ompC"
                     /locus_tag="OO130_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HCR5364738.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNKSEDGDQTYVRLGFKGETQVTDQLTGYGQWEYQIQGNTSEDNKENSWTRVAFAGL
                     KFQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFG
                     LVDGLNFAVQYQGKNGSVSGEGMTNNGRGALRQNGDGVGGSITYDYEGFGIGAAVSSS
                     KRTDDQNGSYISNGVVRNYIGTGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSL
                     GWANKAQNFEAVAQYQFDFGLRPSVAYLQSKGKNLGVINSRNYDDEDILKYVDVGATY
                     YFNKNMSTYVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            8798..9853
                     /gene="apbE"
                     /locus_tag="OO130_001251"
     CDS             8798..9853
                     /gene="apbE"
                     /locus_tag="OO130_001251"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HCR5364739.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MAEIVTTSLRIGAKTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDEMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GINGEGLPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            9927..10979
                     /gene="ada"
                     /locus_tag="OO130_001252"
     CDS             9927..10979
                     /gene="ada"
                     /locus_tag="OO130_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HCR5364740.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANAQQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCALGRCPVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVLANAIGKPKAVRAVASACAANKLAIVIPCHRVVGGDGTLSGYRWGV
                     SRKAQLLRREAENEER"
     gene            10979..11629
                     /gene="alkB"
                     /locus_tag="OO130_001253"
     CDS             10979..11629
                     /gene="alkB"
                     /locus_tag="OO130_001253"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HCR5364741.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTIHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFYPLTTDCRYNLTFRQAGKKE"
     gene            11705..13348
                     /gene="yojI"
                     /locus_tag="OO130_001254"
     CDS             11705..13348
                     /gene="yojI"
                     /locus_tag="OO130_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HCR5364742.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIEMA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLMAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFSYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGKPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            13566..15212
                     /gene="mqo"
                     /locus_tag="OO130_001255"
     CDS             13566..15212
                     /gene="mqo"
                     /locus_tag="OO130_001255"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HCR5364743.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMLERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDADKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLDLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
     gene            complement(15362..15850)
                     /gene="eco"
                     /locus_tag="OO130_001256"
     CDS             complement(15362..15850)
                     /gene="eco"
                     /locus_tag="OO130_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HCR5364744.1"
                     /translation="MKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAEKGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAE
                     VR"
     gene            complement(15985..16149)
                     /locus_tag="OO130_001257"
     CDS             complement(15985..16149)
                     /locus_tag="OO130_001257"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5364745.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYKHIYIAINSPEI
                     FLLVCSLIRI"
     gene            16258..16752
                     /gene="napF"
                     /locus_tag="OO130_001258"
     CDS             16258..16752
                     /gene="napF"
                     /locus_tag="OO130_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HCR5364746.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            16742..17005
                     /gene="napD"
                     /locus_tag="OO130_001259"
     CDS             16742..17005
                     /gene="napD"
                     /locus_tag="OO130_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HCR5364747.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            17002..19488
                     /gene="napA"
                     /locus_tag="OO130_001260"
     CDS             17002..19488
                     /gene="napA"
                     /locus_tag="OO130_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HCR5364748.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTLITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEDLLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTQDATDPLSKETDFKKCAVKLEKV"
     gene            19495..20190
                     /gene="napG"
                     /locus_tag="OO130_001261"
     CDS             19495..20190
                     /gene="napG"
                     /locus_tag="OO130_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HCR5364749.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHRDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            20177..21040
                     /gene="napH"
                     /locus_tag="OO130_001262"
     CDS             20177..21040
                     /gene="napH"
                     /locus_tag="OO130_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HCR5364750.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILLGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AATDRQKCNRCMDCFHVCPEPRVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            21037..21486
                     /gene="napB"
                     /locus_tag="OO130_001263"
     CDS             21037..21486
                     /gene="napB"
                     /locus_tag="OO130_001263"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HCR5364751.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQERMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            21496..22098
                     /gene="napC"
                     /locus_tag="OO130_001264"
     CDS             21496..22098
                     /gene="napC"
                     /locus_tag="OO130_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HCR5364752.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            22117..22734
                     /gene="ccmA"
                     /locus_tag="OO130_001265"
     CDS             22117..22734
                     /gene="ccmA"
                     /locus_tag="OO130_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HCR5364753.1"
                     /translation="MLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHRDG
                     DTAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAIDVN
                     GVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            22731..23393
                     /gene="ccmB"
                     /locus_tag="OO130_001266"
     CDS             22731..23393
                     /gene="ccmB"
                     /locus_tag="OO130_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HCR5364754.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            23435..24172
                     /gene="ccmC"
                     /locus_tag="OO130_001267"
     CDS             23435..24172
                     /gene="ccmC"
                     /locus_tag="OO130_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HCR5364755.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            24169..24378
                     /gene="ccmD"
                     /locus_tag="OO130_001268"
     CDS             24169..24378
                     /gene="ccmD"
                     /locus_tag="OO130_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HCR5364756.1"
                     /translation="MTLAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILCGVEQQRAREARLRAAQQQEAA"
     gene            24375..24854
                     /gene="ccmE"
                     /locus_tag="OO130_001269"
     CDS             24375..24854
                     /gene="ccmE"
                     /locus_tag="OO130_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HCR5364757.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            24851..26800
                     /gene="ccmF"
                     /locus_tag="OO130_001270"
     CDS             24851..26800
                     /gene="ccmF"
                     /locus_tag="OO130_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HCR5364758.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     MLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIAIASLLSGRLDSTYARFTRPWTLAAW
                     IFLTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKA
                     WTLLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAA
                     RGHKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPF
                     FNTMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKV
                     VAMTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQN
                     YSVERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEK
                     RYYNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMAL
                     GGLLCLFDPRYRKRVSPQKTAPEAV"
     gene            26797..27354
                     /gene="dsbE"
                     /locus_tag="OO130_001271"
     CDS             26797..27354
                     /gene="dsbE"
                     /locus_tag="OO130_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HCR5364759.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            27351..28403
                     /gene="ccmH"
                     /locus_tag="OO130_001272"
     CDS             27351..28403
                     /gene="ccmH"
                     /locus_tag="OO130_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HCR5364760.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPDAFPEQSVPEGKRAGYIVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLSANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(28438..29085)
                     /gene="narP"
                     /locus_tag="OO130_001273"
     CDS             complement(28438..29085)
                     /gene="narP"
                     /locus_tag="OO130_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HCR5364761.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPSFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
BASE COUNT         6682 a         7221 c         8105 g         7249 t
ORIGIN      
        1 caactactat ggtgggcgca gcgaaacgga attaaccacc gcccaactga cgctgatcac
       61 cgatgaagga tcggtcaatg agaaacagga aacatttatt gtaccgatgc gtaatgctgg
      121 cgagctgacg ctggtgaaaa gttttgactg gtgatccatc ccttcacaca acctggctct
      181 ctcttttttc agataagact gctgggcgat ctgccgacgc caggatataa ccattaacaa
      241 gcgtcggcag attggtcatt tcttattttt cgtactgaga actgtccatc tactccggct
      301 ttcctggtta aggcaatggt ttctaagcga tacgcattat tgcaataatg acagaacatt
      361 gcggtaacac gcttttaccg ctaccttaac cacactccat cggtcacctg aggcggagct
      421 tcgccccttt gaaatacctt gcttcttttc gtacaaccct gaaagcctcg cgctacatgt
      481 tcagagcatt ggcgttagtg ctctggctgt tgattgcttt ttcatccgtt ttttacatcg
      541 ttaatgcgtt acatcagcga gaatcggaaa ttcgtcagga atttaatctg agttccgatc
      601 aggctcagcg ctttattcaa cgcacctctg atgtgatgaa agagctgaag tacatcgccg
      661 aaaatcgctt atcggcagaa aacggtgtgc tttccccgcg tggacgagaa acgcaggcgg
      721 atgtgcctgc gtttgaaccg ctgtttgccg actccgattg ttccgcaatg agtaacacct
      781 ggcgaggttc tctggagtca ttggcgtggt ttatgcgcta ctggcgcgat aatttttctg
      841 cggcttacga tctaaaccgg gtatttttaa tcggcagcga taacctctgc atggccaatt
      901 tcggtctgcg tgatatgcca gtggaacgcg ataccgcgtt gaaagctttg catgaacgca
      961 tcaataaata tcgaaatgca ccacaagatg atagcggcag taacctctac tggatcagcg
     1021 aaggtccgcg ccctggcgtc gggtattttt acgcgttgac gccagtttat ctggcgaacc
     1081 ggttgcaggc gcttttgggg ggcgagcaga ccatccggat ggagaacttt ttcttaccgg
     1141 gtacgttgcc gatgggggtt accattcttg atgaaaatgg tcataccctg atttcgctta
     1201 ccggaccaga aagtaaaatt aagggcgagc ctcgctggat gcaggaacgc tcctggtttg
     1261 gctatacgga agggttccgg gagctggtgc tgaagaaaaa tctgccaccc tcatcgctaa
     1321 gcatcgtgta ttcggtgccg gttgataagg tgctggaacg cattcgcatg ttgatcctta
     1381 acgcaatttt gctgaatgtg cttgccggag ctgcattgtt tactctcgca cggatgtacg
     1441 agcgacgtat tttcattccg gcggaaagcg acgccctgcg actggaagaa catgagcagt
     1501 tcaatcgcaa gattgtcgcc tccgcgccag tgggtatctg cattttgcgt accgctgatg
     1561 gcgtcaatat tttaagtaac gaactggcgc atacctatct caatatgctt acgcatgagg
     1621 accgccaacg actgacgcaa attatctgtg ggcagcaggt caattttgtt gatgtcctga
     1681 ccagcaacaa taccaatctg caaatcagct tcgtccattc gcgctatcgt aatgaaaacg
     1741 tggccatttg tgtgctggtg gatgtttctt cgcgcgtgaa gatggaagag tcgttgcagg
     1801 agatggcaca agcagcggaa caggcgagcc agtcaaaatc gatgttcctt gccaccgtca
     1861 gtcatgagct gcgaacgccg ctgtatggca ttatcggtaa cctggatctg ttgcaaacca
     1921 aagagttacc gaaaggcgtc gatcggctgg tgacggcaat gaacaactct tccagcctgt
     1981 tgttgaaaat tatcagcgat attctcgatt tctcgaagat tgaatcggaa cagttgaaga
     2041 tcgaaccgcg tgagttttca ccgcgtgaag tgatgaacca catcaccgcc aactatttac
     2101 cgctggtggt acgcaagcag ttaggcttgt actgctttat tgaaccggat gtgccagtgg
     2161 ccttaaatgg cgacccgatg cgtttacagc aggtcatctc caacctgttg agtaacgcca
     2221 taaaattcac cgataccggc tgtatagttt tgcatgtccg cgctgatggc gattatctct
     2281 ctatccgtgt tcgcgatacc ggcgtgggga ttccggcgaa agaagtggtg cgcttgtttg
     2341 atcccttctt ccaggtcgga acgggcgtac agcgaaactt ccaggggacc ggtctgggtc
     2401 tggcgatttg tgaaaaactg atcagcatga tggacggcga tatctcggta gattcagaac
     2461 cgggaatggg cagccagttt accgtgcgta ttccgttgta cggcgctcag tacccgcaga
     2521 aaaaaggcgt ggaagggttg agtggtaaac gctgctggct ggcggtccgc aatgcgtcgc
     2581 tctgtcagtt cctagaaact agtttgcagc gcagcggcat cgtcgttaca acatacgaag
     2641 ggcaggaacc gactcccgaa gatgtgttaa ttactgacga ggtagtgagt aaaaaatggc
     2701 agggtagagc ggtagtgacc ttctgtcgtc gccatattgg tattccgctg gagaaagcgc
     2761 caggggagtg ggtacacagt gtggctgccc cgcatgagct accggcattg ttggcgcgta
     2821 tttatttgat cgagatggag agcgacgatc ctgctaacgc tctgccgtcg acggacaaag
     2881 cggtcagcga taatgacgat atgatgattc tggtcgtgga tgatcatccg attaaccgac
     2941 gtctgctggc agatcagttg ggatcgttgg gctatcaatg taaaaccgcg aatgatggtg
     3001 tcgatgcgct taatgtactt agcaagaatc atattgatat cgtgcttagc gacgtcaaca
     3061 tgccaaatat ggatggttac cgcttgacgc aacgcattcg tcagttggga ctgacgttgc
     3121 cggtaatcgg agtaactgct aatgcgttgg ctgaagagaa gcagcggtgt ctggagtccg
     3181 gtatggacag ctgcctgtcg aagccggtaa cgctggatgt gataaaacag acgctgacgg
     3241 tatatgccga gagggtcagg aaatcgcggg aatcgtagga cggataaggt gtttatgtcg
     3301 catctggcat tcagtgcaaa tgccagatgc gacgctgacg cgtcttatct ggcctacttt
     3361 gatgtaatct gttgaaataa tgggaatcgt aggacgcata aggcgtttac gccgcatccg
     3421 gcaatcggtg caaatgctag ataagacact aacgcgtctt atctggccta caggtgatta
     3481 gtctttatct gccggactta acgtcactga agagagataa ttcagcaggg cgatatcgtt
     3541 ctcgacaccc agcttcatca tcgcagattt cttctggcta ctgatggttt taatactgcg
     3601 gttcagcttt ttagcgatct cggtcaccaa gaagccttcc gcaaacaggc gcagaacttc
     3661 actctctttt ggcgagagac gcttgtcacc gtaaccacca gcactgattt tttccaacag
     3721 gcgagaaacg ctttccgggg taaatttctt ccctttctgc agcgcggcga gagctttcgg
     3781 cagatcggtc ggtgcacctt gtttcagcac gatcccttcg atatccagat ccaataccgc
     3841 actaagaatc gccgggttgt tgttcatagt cagaacaatg atcgacaggc ttgggaaatg
     3901 gcgcttgatg tacttgatta aggtaatgcc atcgccgtac ttatcgccag gcatggagag
     3961 atcggtaatc aacacatgcg catccagttt cggcaggttg ttgatcagtg ctgtagagtc
     4021 ttcaaattcg ccgacaacat tcacccactc aatttgctca agtgatttgc gaataccgaa
     4081 caagactatc ggatggtcat cggcaataat tacgttcata ttgttcatgt aataggctac
     4141 cttgctacag caagctcttg acataactgt caatgtcgct gatgtatttt tctattcctg
     4201 gaacatcctt ctcacgaatc agatgttcca gcgtttcaca taactgcttg ccgggtacca
     4261 gatttagcat ggcaaatacg cctttaagac gatgagccgt ttgggctaac gcagcaaagt
     4321 cactggttgc tgcttcagta tacagcctct taacatcatc cggtactgtg tctacaaaga
     4381 gcgcataata gccgctggca tggagttgcg cattttcatc tccgcccaga ggcgattctg
     4441 tcacctcttc ctgcgccagt tgcacttcaa ttaattgtaa gaccgcttcc tgcatagcgt
     4501 tgctcatatt gaagttgacg cacaattgac caggcccaat ttcccgtacg ccagactcat
     4561 catcgcttaa aagcaagcca gaggcagtaa gattagacgg attatccgtt aaaaagatat
     4621 cataatcttg actaattaat ctttcatcgg gtgtgataca ggttgcaccc caattttcta
     4681 actggcgagt gacaatattc cgaatttctg ccgaagtaac atccaccatt acgcagacat
     4741 catccagtaa acgctcttct tcctcttcaa cttccgggtc agcggcgagc attttgatat
     4801 gcacagagta gcgtgtacca agcccatccc gcgttttgat gtttaaatga ccgcccagtt
     4861 tacgcgccag ttgatcgctc agccagaatg ccagcgggtc cgccttgcca tagcgatcgt
     4921 tttggggctg gttgataaac gggaagtgca aattatccat ttcatgaata cttacgcctt
     4981 ctcccgtgtc cagaatgcgg aatgtcaggc ggtcttcaga ggactcatcc tgatcaacct
     5041 caagggtgat ttttcccaat tgcgttgagg tcacggcata ttgcatcagt agcagcaaaa
     5101 tacgtcgtaa ggcatcgcga tcgccgcggc gcatatcgtg tgctttcaga tgattgttaa
     5161 tcagcagttg cagaccttta cgcttgatgg caggcaacac tgaaggcaca acttcatcaa
     5221 ttaaatcctg cacggagaac agcaccgtct cacttttcca gctatcgtcc gcaagcatgt
     5281 tcgctaactg tatttcatcg accagccgca ccagcacatc tgcctgattc gccagttgtt
     5341 tgctttccgg ggcgttgagt ttagccgcgc tctccgccag ggactgtgcg ggttctttca
     5401 gcgcatcgcc aatgtttttc ataaaggtca tccgcccctg ctggtttttc tcatacagac
     5461 gctgcgcctg cttgagtttc ttgtttacca gcacttcgcg atcctgatcg cgaataatga
     5521 aaatttgtgt gcgcggcgcg acctggctgc ggaacatgcg gatctcatac agctcgttat
     5581 tgatcgtcgc ctgaataatc ccctgatgct gttccgccat ggtggtgatg ttttgcagat
     5641 tcaaatgcgg cagcaaatga tcggcaattt tgttacttat gacagtgcgg ttcgattcct
     5701 gatcgtgaac cagcaggccg agcggcagca gtgagactat ctcttcatta attgcccgta
     5761 aaatgcgcaa ttcgttattg accgccgtgc tgggtagact ttctgtactg cggctggaga
     5821 aatggcggaa tgtggtatag ccaaataacg ccagcgccag caaaccgatg ttcagcagca
     5881 gtggcagcag aatgttttgc aacgtatcca gcaataaggt gccataagga acctgccaga
     5941 ccaggcgcat atcggtagag ttgagtgccg aggagatttc aatcttcgta ctgttaaagt
     6001 ggatactgac gctatccgtc ccttctttct cattatcatt gtctcccgtt gccgtcgcgt
     6061 ctggctcaag gcggaaactg tccagcggca tacccggtgg gatcaaatca ttaatcggta
     6121 gatcaaaagc tacgaccgtt gccagatgtc ccggctggtt gaacgtggta cgcaaggtaa
     6181 agtaatgacc gttctgccag gccaggcggc gcatgttaga aaaactttcg cgttcatcga
     6241 gggcgttggc ctgttgcaac atctctgcac gacgtgaatc aacaatgtcg ctgatggtcg
     6301 attctttaaa tccggaggtg agatctttga ggggcagggt tgagatcagc accagactgt
     6361 tatcctgacc attcaggtaa tacatcgacc acggtacatt ttctgcgccc cacaatgtgt
     6421 ccagataggt ggacatccgc tgagtcatct caagcgttga gttgtcgtga gagccaaaga
     6481 ttaacgcttc cgttttgcgg cgcggtttct ccagatagta gacatcctgt ttcaggcgcg
     6541 tctcttgtaa accttcgccg gaggaggggg aggttgtcgc ggcaatgttg tcgtagatct
     6601 gccaggtcac gtaacgccag gtattgacgc gcttttggat agcgtgggta atgtcgacaa
     6661 tctggtaact tttatctttc agccatgcgt taacggcgct ttgtaccatt acacccatcg
     6721 tcaccagtaa cacaatgatc aacagtaaaa agaagcgggt aatgctcccc ggtaggagtg
     6781 aaaagcgggt cgtggccgtt gtctctttct gacgcattcg tgtttatgac ctgttaaaac
     6841 ttcgcgaagt gatagggcag tataaagggt acagtgtgaa attccagact cttacctgat
     6901 tgatgattct ctattctccc gtgatattta tcatcctgca ttgggggaaa tagatgtaca
     6961 agcgccattt tgctgtacaa ataacctaca aaaagcccaa cgaaattaat tgcaaataaa
     7021 cgatgattgc tggaaattat gctgatgtta attatttgtg aaatagttaa caagcgttat
     7081 agtttttctg tggtagcaca gaataatgaa aagtgtgtaa agaagggtaa aaaaaaccgg
     7141 atgcgaggca tccggttgaa ataggggtaa acagacattc agaaatgaat gacggtaata
     7201 aataaagtta atgatgatag cgggagttat tctagttgcg agtgaaggtt ttgttttgac
     7261 attcagtgct gtcaaatact taagaataag ttattgattt taaccttgaa ttattattgc
     7321 ttgatgttag gtgcttattt cgccattcca caataatctt aaaaagttcc cttgtattta
     7381 cattttgaaa catctatagc gataaatgaa acatcttaaa agttttagta tcatattcgt
     7441 gttggattat tctgcatttt tggggagaat ggacttgccg actgattaat gagggttaat
     7501 cagtatgcag tggcataaaa aagcaaataa aggcatataa cagagggtta ataacatgaa
     7561 agttaaagta ctgtccctcc tggtcccagc tctgctggta gcaggcgcag caaacgctgc
     7621 tgaagtttac aacaaagacg gcaacaaatt agatctgtac ggtaaagtag acggcttgca
     7681 ctatttctct gacaacaagt cagaagacgg cgaccagacc tatgtacgtc ttggtttcaa
     7741 aggcgaaact caggttactg accagctgac cggttacggc cagtgggaat atcagatcca
     7801 gggcaatacc tctgaagaca acaaagaaaa ctcctggacc cgtgtggcat tcgcaggtct
     7861 gaaattccag gatgtaggtt ctttcgacta cggtcgtaac tacggcgttg tttacgacgt
     7921 aacttcctgg accgacgtac tgccagaatt cggtggcgac acctacggtt ctgacaactt
     7981 catgcagcag cgtggtaacg gcttcgcgac ctaccgtaac accgacttct tcggtctggt
     8041 tgacggtctg aactttgctg ttcagtacca gggcaaaaac ggtagcgtaa gcggcgaagg
     8101 catgaccaac aacggtcgtg gcgctctgcg tcagaacggc gacggtgtcg gcggttctat
     8161 cacttatgat tacgaaggct tcggtatcgg tgctgcagtt tccagctcca aacgtactga
     8221 tgatcaaaat ggtagctaca tcagcaatgg tgtagttcgt aactacatcg gtactggcga
     8281 ccgtgctgaa acctacactg gtggtctgaa atacgacgct aacaacatct acctggctgc
     8341 tcagtacacc cagacctaca acgcaactcg cgtaggttcc ctgggttggg cgaacaaagc
     8401 acagaacttc gaagctgttg ctcagtacca gttcgacttc ggtctgcgtc cgtctgtagc
     8461 atacctgcag tctaaaggta aaaacctggg tgtcatcaat agtcgtaact acgacgacga
     8521 agatatcctg aaatatgttg atgttggcgc gacctactac ttcaacaaaa acatgtccac
     8581 ctatgttgac tacaaaatca acctgctgga cgacaaccag ttcactcgtg acgctggcat
     8641 caacactgat aacatcgtag ctctgggtct ggtttaccag ttctaatctc gattgatatc
     8701 gaacaaaggg cctgcgggcc cttttttcat tgttttcagc gtacaaactc agttttttgg
     8761 tgtactcttg cgaccgttcg catgaggata atcacgtatg gaaataagct ttacccgcgt
     8821 ggcactgctg gctgccgcgc tcttctttgt tggttgcgat caaaaaccac aacccgccaa
     8881 aacccacgct actgaagtta ccgttcttga aggcaaaacc atgggtacct tctggcgtgc
     8941 cagcatcccg ggcattgatg ccaaacgcag tgccgaactt aaagaaaaga ttcagaccca
     9001 gctggacgcc gacgatcagc tgctttcgac ctataaaaaa gattccgcgc tgatgcgctt
     9061 taacgactcg caaagtttgt cgccgtggcc agtaagtgaa gcgatggccg aaatcgtcac
     9121 tacctcgctg cgcattggcg cgaagaccga tggtgcgatg gatattaccg tcgggccgct
     9181 ggtaaatctg tggggctttg gcccggaaca acagccggtt caaattccga gccaggaaca
     9241 gatcgacgag atgaaagcca aaactggttt acagcacctt acggtcatta atcagtcgca
     9301 tcagcaatat ctgcaaaaag acctgccgga tttatatgtc gatctctcta ccgtcggcga
     9361 aggttatgcg gcggatcact tggcacgctt gatggagcag gaagggattt cccgctatct
     9421 ggtgtcggtg ggcggcgcgc tgaacagccg tggtatcaac ggtgaaggcc tgccgtggcg
     9481 ggtagcgatt caaaaaccaa ccgataaaga aaacgcagtt caggctgtgg tggatatcaa
     9541 cggtcatggg attagcacct ctggcagcta ccgtaactat tacgaactgg acggcaaacg
     9601 tctttcccat gttatcgatc cgcaaaccgg gcgtcccatc gaacacaatc tggtatccgt
     9661 gacggtgatt gctccgacgg cgctggaagc cgatgcctgg gatactggct tgatggtact
     9721 cgggccggag aaagccaaag aggttgttcg ccgggaaggg ctggcggttt atatgatcac
     9781 caaagaaggc gatagcttta aaacctggat gtcaccacag tttaaaagct tccttgtcag
     9841 cgaaaaaaat taaagcgcaa gattgttggt ttttgcgtga tggtgaccgg gcagactaaa
     9901 ggctatcctt aaccagggag ctgattatga aaaacgccac atgcttaact gacgatcaac
     9961 gctggcaatc tgtcttagcc cgcgacccga atgccgacgg cgaattcgtt ttcgccgtgc
    10021 gcaccacagg catcttttgc cgtccgtctt gccgcgccag acatgccttg cgggaaaatg
    10081 tctccttcta cgcaaatgcc agcgaggcac tcgccgccgg atttcgcccc tgcaaacgct
    10141 gccaacctga taaagccaat gcccagcaac atcggttgga taaaatcacc cacgcgtgtc
    10201 gactgctgga acaggaaacg cctgtaacgt tggaagcctt agccgaccag gtggcgatga
    10261 gtccatttca tctacatcgg ttgtttaaag cgactaccgg aatgacgcct aaagcctggc
    10321 aacaggcctg gcgcgctcgc cgtttgcgcg aatcgctggc gaaaggggag agcgtgacga
    10381 cgtctattct taacgccgga ttccccgaca gcagcagtta ctaccgcaaa gccgatgaaa
    10441 cgctgggcat gacggctaaa caattccgtc acggtggcga aaatctggcg gtgcgttacg
    10501 cgctggctga ttgtgcgctg ggtcgttgcc cggtggcaga aagcgagcgg gggatttgcg
    10561 cgatattgct gggcgatgat gacgcgacat taatcagcga gttgcagcag atgtttcccg
    10621 ctgccgacaa cgcgcctgcc gatctgacgt ttcagcaaca tgtgcgtgaa gtgatcgcca
    10681 gcctcaatca acgcgatacg ccgctgacgt taccgctgga tattcgcggc actgcttttc
    10741 agcaacaagt ctggcaggca ctgcgcacga taccttgcgg tgaaaccgta ctggctaacg
    10801 ccatcggcaa accgaaagcg gtacgggccg ttgccagcgc ctgtgccgcc aacaagctgg
    10861 ctatcgtaat accctgtcat cgagtggtcg gtggtgatgg cacactttcc ggttaccgct
    10921 ggggcgtgtc tcgtaaagcg caactgctgc gccgcgaagc tgaaaatgag gagaggtaat
    10981 gttggatctg tttgccgatg ctgaaccgtg gcaagagcca ctggcggctg gtgcggtaat
    11041 tttacggcgt tttgctttta acgctgcgga gcaactgatc cgcgatatta atgacgttgc
    11101 cagccagtcg ccgtttcgcc agatggtcac ccccggggga tataccatgt cggtggcgat
    11161 gaccaactgt gggcatctgg gctggacgat ccatcggcaa ggttatctct attcgcccat
    11221 tgatccgcaa acaaataaac cgtggcccgc catgccacaa agctttcatg atttatgtca
    11281 acgtgcggct acggcggcgg gctatccaga tttccagcca gatgcttgtc ttatcaaccg
    11341 ctacgctcct ggcgcgaaac tgtcgctgca tcaggataaa gacgaaccgg atctgcgcgc
    11401 gccaattgtt tctgtttctc tgggcttacc cgcgattttt caatttggtg gcctgaaacg
    11461 taatgatccg ctcaaacgtt tgttgttgga acatggcgat gtggtggtat ggggcggtga
    11521 atcgcggctg ttttatcacg gtattcaacc gttgaaagcg gggttttatc cactcaccac
    11581 cgactgccgc tacaacctga cattccgtca ggcaggtaaa aaagaataaa aataagaatt
    11641 attattgctg tgcgcgcgaa gatgtttaaa ctgcgggctg taattcattg tccgggtttt
    11701 ctgcatggaa cttcttgtac ttgtctggcg gcagtatcgc tggccattta tcagtgtgat
    11761 ggcgctaagc ctcgccagtg cggcattagg cattggctta attgctttta tcaatcagcg
    11821 ccttatcgaa atggcggata ccagtctgct ggtattgccg gagtttctgg gattattgct
    11881 gctgttgatg gcagtcactc tcggatcgca actggcgctc accactttgg ggcatcactt
    11941 cgtttaccga ctgcgtagtg aatttatcaa gcggattctg gatacccacg tcgagcgcat
    12001 tgaacaactc ggtagcgcct cgttgttggc gggattaacc agcgatgtgc gcaatatcac
    12061 cattgctttt gtgcgtcttc cggaactggt gcaggggatc attctcacta tcggttccgc
    12121 ggcgtatctg tggatgctgt cgggcaaaat gttgctggta acggctatct ggatggcgat
    12181 caccatctgg ggtggttttg tactggtggc gcgggtgtac aaacatatgg cgaccctacg
    12241 tgaaaccgaa gacaagctgt acacggattt tcaaactgta cttgaagggc gcaaagagct
    12301 gactctgaac cgggaacgcg ccgagtatgt gtttaacaac ctctacatcc ccgatgcgca
    12361 agagtatcgc catcatatta ttcgcgcaga caccttccat cttagtgccg tgaactggtc
    12421 aaacatcatg atgctgggcg ctatcggcct ggtgttctgg atggcgaaca gtctcggttg
    12481 ggctgatacc aacgttgctg cgacctattc gttgacgctt ttattcctgc gtacgccgct
    12541 gctttcggcg gttggcgcat tgccgacgct gctgatggcg caggtggcgt ttaacaagct
    12601 gaacaaattc gcgctcgcgc ctttcaaagc agagtttccg cgcccgcagg cgtttcccaa
    12661 ctggcaaacg ctggagctgc gtaatgtgac gtttagctat caggataacg cgttttccgt
    12721 tggtccgatt aatctcacca tcaaacgtgg cgagctactg tttctgattg gtggtaacgg
    12781 tagtggaaaa tcgacgctgg cgatgttgtt gacgggcttg tatcagccac aaagcggcga
    12841 aatcttgctg gatggcaaac ctgtcagcgg cgaacaaccg gaagattatc gcaaactgtt
    12901 ttcggcagtg tttaccgatg tctggctttt tgatcaactg ctggggccgg agggtaaacc
    12961 cgctaacccg caactggttg agaagtggct ggcgcagctg aaaatggctc ataagcttga
    13021 gttaagcaac gggcgtattg ttaacctgaa gttatcaaaa gggcagaaaa aacgcgtggc
    13081 gctgttgctg gcgctggcag aagaacgcga tattatcctg ctggatgaat gggcggcgga
    13141 tcaggatcca cacttccgtc gtgagtttta tcaggtgttg ctgccgctga tgcaggagat
    13201 gggtaaaact attttcgcta tcagtcatga tgatcattac tttatccacg ccgaccgcct
    13261 gctggaaatg cgcaatgggc aacttagcga gctgacgggc gaagagcgcg atgccgcttc
    13321 gcgtgatgcc gttgcccgga cggcataata ttacgacgct gacctggcgg actgctgccg
    13381 tcaggtcaat atgctaaaaa tcccttcatt tctaataacc ctataattaa tcaacgaaat
    13441 tataatgttt ctaaaattag aatataattt ataaacatta tttaaatgtt gttacttaag
    13501 tgttaccttt gatgccgcgc aaatctcacc tgcaataaag cgactaaaag taaggcatta
    13561 acaagatgaa aaaagtgact gccatgctct tctcgatggc cgtggggctt aatgccgttt
    13621 cgatggcggc aaaagcgaaa gcgtccgagg agcaggaaac tgatgtactg ttgattggcg
    13681 gcggcattat gagcgccacg ttggggacct atttacgcga gctggagcct gaatggtcga
    13741 tgaccatgct ggagcgcctg gagggtgtcg cgcaggagag ttcgaacggc tggaataacg
    13801 ccggaaccgg gcattctgca ctgatggaac tgaactacac cccgcaaaac gccgatggca
    13861 gtatcagtat tgaaaaagca gtcgccatta acgaagcatt tcagatttcc cgccagttct
    13921 gggcgcacca ggtcgagcgc ggcgtgctgc gtactccgcg ttcatttatc aataccgttc
    13981 cgcatatgag ctttgtctgg ggcgaggata acgttaattt cctgcgcgcc cgttacgccg
    14041 cgttgcaaca aagctcgctg tttcgcggta tgcgttactc tgaagatcac gcgcagatca
    14101 aagagtgggc accgttagtg atggaagggc gcgatccgca acagaaagtg gcagccacgc
    14161 gtacggaaat tggtaccgat gtgaactacg gcgagatcac ccgccagtta attgcttcct
    14221 tgcagaagaa atctaacttc tcgctgcaac tcagcagcga agtccgcgcc ctaaagcgta
    14281 atgacgataa cacctggacc gttaccgttg ccgatctgaa aaatggcact gcacagaaca
    14341 ttcgtgccaa atttgtcttt atcggcgcgg gcggtgcggc gctgaagctg ttacaggaat
    14401 cggggattcc ggaagcgaaa gactacgccg gtttcccggt gggcggacag ttccttgttt
    14461 cggaaaaccc ggacgtggtt aatcaccatc tggcgaaggt ttacggtaaa gcatccgttg
    14521 gcgcaccacc gatgtcggtt ccgcatatcg atacccgcgt tctggacggt aaacgcgtag
    14581 tgctgtttgg gccatttgcc accttctcaa ccaaattcct caaaaacggt tcattgtggg
    14641 atctgatgag ttccaccacc acctctaacg tgatgccgat gatgcacgtc gggctggata
    14701 atttcgatct ggtgaaatat ctggtgagtc aggtgatgtt gagtgaagag gatcgttttg
    14761 aagcgttgaa agagtactat ccgcaggcga aaaaagagga ctggcgtttg tggcaagcgg
    14821 ggcagcgtgt gcagattatc aagcgtgatg ccgataaagg tggtgtactg cgtctgggta
    14881 ctgaagtggt cagtgaccag caagggacca ttgccgcgct cctgggagca tcgccagggg
    14941 cgtcaaccgc cgcgccgatt atgttggatc tgctggaaaa agtatttggc gatcgcgttt
    15001 ccagcccgca atggcaggct acgttgaaag cgatcgttcc gtcttatgga cgcaagctga
    15061 acggtgatgt agcggcaaca gaacgcgagt tgcagtacac cagcgaagtg ctggggctga
    15121 aatacgacaa gccgcaggca gcagatagta cgccaaaacc gcagttgaaa ccgcaacccg
    15181 ttcaaaaaga agtggcggat attgcgttgt aatgatgtgc cacatccggc atggtatgcc
    15241 ggatgtggcg tatactgata agacgcgcca gcgtcgcatc aggcaaccgg ctcgggcgtt
    15301 agatgttgga tgcggcgtaa ccgccttatc caaccaacgg ggtgccgtac ttcacaacag
    15361 tttagcgaac ttctgcgttg tcaattttct cttccgcctt ccagacgcgg tatttcacat
    15421 ctacattgtc tggcgtatac accacgatcg gcagcttgct gttgtaacgc agcattccag
    15481 catcgcccag atacgcggtg acaaatttct tctctttctt gccatccggg caggccatca
    15541 tcgtggaaac cggcgagcta actttatcaa agacataata atcatagccc cagccttcca
    15601 gcgttttgct ttccagcttc ccgccgagac gatgcaaatt gcaatcgact tccagcgtct
    15661 gaccgattaa cagttctact ttcagggtag attcatcttc ttgcggggtt aactgaatca
    15721 cctgacgctt catccctttt tcagcttgtg gataaggcgc gattttttcc agtggctgga
    15781 cgctttctgc cgcccaggcg gaagtggtag cgaaagcggc aaacaatact gcaggtagaa
    15841 tggtcttcat ttttttgctt tccttcttca tttataggtt ttcaccgatg atgtcgctga
    15901 aggttaacat attcataaga tttattaatt tgctttatga ataacttaaa gaatattcag
    15961 acaattttac ctgctctttt agtgttaaat tctgatgaga gaacaaacca gcagaaatat
    16021 ctctggtgaa ttaatcgcta tataaatatg tttataacca tttgaaatgt gagcaaaagc
    16081 ccgtttttgc cacgcttccc gccactcttt tgatcctgct acaggtttta ccccgatcgg
    16141 ggtatgcatc tttgacacat cctttaatat cttagcggtt ataaaaatgg cttattaatt
    16201 atgcggttta tttggtcgct ctcaattttc agagcgcgtt aatgatggaa ggtcaatgtg
    16261 aagattgatg catcccgtcg gggcatactc actggtcgct ggcgcaaagc cagtaacggt
    16321 atccgtccgc cctggtccgg tgatgaatct cattttctga cccattgcac ccgttgcgac
    16381 gcctgtatta atgcttgtga aaacaacatt ctgcaacgcg gcgcgggcgg ctatccgagc
    16441 gttaatttca aaaataacga gtgcagcttc tgttacgctt gcgcgcaggc ttgccccgaa
    16501 tcgctatttt ctccgcgcca caccagggcc tgggatttgc agttcaccat tggggacgcg
    16561 tgcctggcgt atcagtcagt tgaatgccgc cgctgtcagg atagctgtga gccaatggca
    16621 attatctttc gcccgacgct ctccgggatc taccagccgc aacttaatag ccaactttgc
    16681 aacggatgtg gagcatgtgc agccagctgt ccggtatcag ccataactgc ggagtatctc
    16741 catgcacact aactggcaag tttgcagcct ggtcgtgcag gccaaaagcg aacgaatttc
    16801 agacatcagc acccaactga acgcctttcc cggctgtgaa gttgctgtca gcgacgcgcc
    16861 gagcggtcag ttgattgtgg tggtggaagc agaagacagc gaaacgctga tccaaaccat
    16921 tgagtcagta cgcaacgtag agggcgtgct ggcggtgtcg ctggtttatc accagcagga
    16981 agagcaaggt gaggaaacac catgaaactc agtcgtcgta gctttatgaa agctaacgcc
    17041 gttgcggccg ctgcggcggc tgccggtctc agcgtgccgg gcgttgcccg cgccgttgtt
    17101 ggtcagcagg aagccatcaa atgggataaa gcgccgtgcc gtttctgcgg tactggttgc
    17161 ggcgttctgg tcggaacgca gcagggacgt gtggtggcct gtcagggcga cccagacgca
    17221 ccggttaacc gtggcctgaa ctgcattaag ggctatttcc tgcccaagat catgtacggt
    17281 aaagaccgtt tgacgcagcc gctgctgcgt atgaaaaacg gtaaatatga caaagaaggc
    17341 gaatttaccc taatcacctg ggatcaggcc ttcgatgtga tggaagagaa attcaaaacc
    17401 gccctgaaag aaaaagggcc ggaatcgatc ggtatgttcg gttctggtca gtggactatc
    17461 tgggaaggtt atgccgcgtc caagctgttt aaagcgggct tccgttcgaa caacatcgac
    17521 ccgaacgcgc gtcactgtat ggcgtcggca gtagttggct ttatgcgtac ctttggtatg
    17581 gatgagccga tgggctgcta tgacgacatc gagcaggctg acgcgtttgt gctgtggggc
    17641 gcaaacatgg cggagatgca cccgatcctc tggtcgcgca tcactaaccg tcgtctctct
    17701 aatcagaacg tcaccgtggc ggtgctttct acctaccagc atcgtagctt cgagctggcg
    17761 gataacggca tcatctttac gccacagtct gacctggtga tcctgaacta catcgccaac
    17821 tatatcattc aaaacaatgc gataaatcag gacttcttca gcaaacacgt taacctgcgc
    17881 aaaggggcga cggacattgg ctacggttta cgtccgaccc atccgctgga aaaagcagcg
    17941 aagaatccag gttctgacgc ctccgaaccg atgagctttg aagattacaa agccttcgtt
    18001 gccgagtata cgctggaaaa aactgccgaa atgaccggcg tgccgaaaga ccagttagaa
    18061 caactggcgc agctgtatgc cgatccgaac aagaaagtca tctcctactg gacgatgggc
    18121 ttcaaccagc atactcgtgg cgtgtgggct aacaacctgg tctacaacct gcacctgctg
    18181 accggcaaaa tttcccagcc gggttgcggt ccgttctccc tgaccgggca accttctgcg
    18241 tgtggtactg cgcgtgaagt gggcaccttt gctcaccgtc tgcctgcgga catggtggtg
    18301 accaacgaga aacaccgtga tatctgcgag aagaagtgga atatcccgag cggcaccatt
    18361 ccggcgaaaa tcggtctgca cgcggtggca caagaccgtg cgctgaaaga cggcaaactg
    18421 aatgtttact ggaccatgtg taccaacaac atgcaggccg ggccgaacat caatgaagag
    18481 cgtatgccgg gctggcgcga tccgcgcaac ttcatcatcg tctccgatcc gtatccgaca
    18541 gtcagtgcgc tggccgccga cttgatcctg ccgaccgcaa tgtgggtaga gaaagagggc
    18601 gcttacggta acgccgaacg ccgtactcag ttctggcgtc aacaggtaca ggcaccgggc
    18661 gaagcgaaat cggatctctg gcagttagtc cagttctccc gccgcttcaa aactgaagaa
    18721 gtatggccgg aagatctgct ggcgaagaaa ccggaactgc gtggcaaaac gctgtacgaa
    18781 gtgctgtatg ccacaccgga agtgagcaaa ttcccggtat ccgaactggc ggaagatcag
    18841 ctgaacgatg aatcccgcga gctgggcttc tatctgcaaa aagggctgtt cgaagagtac
    18901 gcatggtttg gtcgcggtca cggtcacgat ctggcaccgt tcgatgacta ccacaaagcg
    18961 cgcggtctgc gctggccggt ggtgaacggt aaagaaacgc agtggcgtta cagcgaaggt
    19021 aacgacccgt acgtgaaagc gggcgaaggc tataagttct acggcaaacc ggatggcaaa
    19081 gcggtgatct tcgcactgcc attcgaaccg gcggcggaag caccggatga agagtacgac
    19141 ctgtggctct ctaccggacg cgttctggag cactggcaca ccggcagtat gactcgccgt
    19201 gtaccagaac tgcaccgcgc cttcccggaa gcggtcctgt ttattcaccc gctggatgcg
    19261 aaagcgcgcg atctgcgtcg tggcgacaaa gtgaaagtgg tttctcgccg tggcgaagtg
    19321 atctcgattg tcgaaacgcg cggtcgtaac cgtccgccgc agggcctggt gtacatgccg
    19381 ttcttcgacg ccgcacagct ggttaacaaa ctaacgcagg acgcgaccga tccgctctcg
    19441 aaagagacgg acttcaagaa gtgcgcagtc aaactggaga aggtgtaagc cattatgtcc
    19501 cggtcagcga aacctcaaaa tggtcgccgc cgctttctgc gcgatgttgt tcgcacagca
    19561 ggcgggctgg ctgccgtggg tgtggcgctg gggttacaac agcaaaccgc acgcgcatct
    19621 ggcgtgcggt tgcgcccgcc cggagccata aacgagaacg cctttgccag tgcctgtgtg
    19681 cgttgcggtc agtgtgttca ggcttgccct tacgacacct taaaactggc gacgctggcc
    19741 tctggtctgt cggcgggcac gccatatttt gtcgcacggg atattccttg cgaaatgtgt
    19801 gaggacattc cgtgcgccaa agtgtgcccg agcggtgcgc tggatcgtga gatcgaatcg
    19861 atcgacgacg cgcggatggg gctggcggta ctggtggacc aggaaaactg tctcaacttt
    19921 caggggctgc gctgcgatgt ttgttatcgc gaatgcccga aaattgatga ggccatcacc
    19981 ctggagctgg agcgcaacac gcgtaccggt aagcacgccc gctttctgcc gacggttcac
    20041 agagacgcct gtaccggttg cggtaagtgc gaaaaagtgt gcgtgctgga acaaccggca
    20101 atcaaggtgt taccgctgtc actggctaaa ggggagttag gtcaccatta ccgcttcggc
    20161 tggctggagg ggaacaatgg caaatcgtaa acgtgacgcc gggcgcgagg cgctggagaa
    20221 aaaaggctgg tggcgcagtc accgttggct ggtgttacgt cgcctttgtc agttcttcgt
    20281 gctggggatg tttttgagcg gtccgtggtt tggtgtgtgg atcttacacg gtaactacag
    20341 cagtagcctg ttattcgaca ccgttccgct gaccgatccg ctgatgacgc tgcaaagtct
    20401 tgccagcggt catctgcctg ccacggtggc gttgaccggt gcggtgatta tcaccgtgct
    20461 ctacgccctg gcaggaaagc gattattttg cagctgggtc tgcccgctga atccgattac
    20521 cgacttagcg aactggttac gcaggcgctt tgacctcaat cagtctgcga cgatccctcg
    20581 ccacattcgc tacgtgctgt tggtggtcat tctgctggga tcggccttaa ctggcacgct
    20641 catctgggaa tggatcaacc ccgtatctct gatggggcgc agcctggtaa tgggcttcgg
    20701 cagcggcgcg ctgcttattc tcgcgctgtt tttatttgat ctactggtcg ttgaacacgg
    20761 ctggtgcggg cacatttgtc cggttggcgc gctgtatggc gtgctgggta gcaaaggtgt
    20821 gattaccgtt gcggccaccg atcggcagaa atgtaaccgc tgtatggatt gttttcatgt
    20881 ttgcccggaa ccgcgtgtgc tacgtgcccc ggtgctggat gaacaaagcc cggtgcaggt
    20941 caccagccgc gattgcatga cgtgcggtcg ctgcgtggat gtctgttctg aggatgtatt
    21001 tacaataact acacgatgga gttcgggagc gaaatcatga aaagccatga cctgaagaaa
    21061 gcgctgtgtc aatggacggc gatgctggcc ctggtggtaa gcggcgcggt ttgggcggct
    21121 aatggcgtcg attttagcca gtcgccggaa gtatccggga cacaggaagg ggccattcgg
    21181 atgccaaaag agcaggaacg gatgccgctg aactatgtga atcagccgcc aatgatcccg
    21241 catagcgttg aaggttatca ggtaacgacc aataccaacc gctgcttgca gtgccacggt
    21301 gtcgaaagtt atcgcaccac tggcgcgccg cgcattagtc ctactcactt tatggacagc
    21361 gacggcaaag tgggcgcgga agtggctccg cgtcgttatt tctgtctgca atgtcacgta
    21421 ccgcaggccg ataccgcgcc aatcgtgggg aataccttta ccccatcaaa aggttacggg
    21481 aaataagagg ttattatggg aaattctgac cgtaagcctg gtctgattaa gcgcctgtgg
    21541 aaatggtggc gtacccccag ccgtctggcg ctggggacgc tgctgttgat cggttttgtt
    21601 ggcggcatcg tcttctgggg tggctttaac accgggatgg aaaaagccaa taccgaagag
    21661 ttctgcatta gctgccacga aatgcgcaac acggtgtatc aggaatacat ggattccgtg
    21721 cactacaaca accgtagcgg cgtccgtgcg acctgtccgg attgtcacgt tccgcacgag
    21781 tttgtgccga agatgatacg caagctcaaa gcaagtaaag agctgtatgg taaaattttt
    21841 ggcgttattg acacgccgca gaaatttgaa gctcatcgtc tgacgatggc acagaatgag
    21901 tggcggcgca tgaaggacaa taactcgcag gagtgccgta actgtcacaa cttcgagtat
    21961 atggatacaa ccgcccagaa atcggttgcc gcgaagatgc atgaccaggc ggtgaaagat
    22021 gggcaaacct gtattgattg ccataaaggg atagcgcaca agctgcccga tatgcgtgaa
    22081 gtcgagccag gtttttaaca gggttattgc gtgggtatgc ttgaagccag agagttactt
    22141 tgtgagcggg atgaacgaac cttatttagt ggcttgtcat ttacgctgaa cgcaggagag
    22201 tgggtacaaa tcaccggtag caacggcgcg gggaagacaa cgcttctccg tttgctgacg
    22261 gggttgtctc gccctgacgc aggcgaggtt ctctggcaag ggcagccctt gcatcaggta
    22321 cgcgacagct accatcaaaa cctgttatgg ataggccatc agccggggat caaaacccgg
    22381 ctgacggcgt tagaaaatct gcacttttat catcgcgatg gcgataccgc acaatgtctg
    22441 gaagccctgg cgcaggccgg gcttgccgga ttcgaagata ttcctgtaaa tcagctctcg
    22501 gccgggcaac aacgccgcgt cgctttagcg cgtctgtggc tgacccgtgc cacgttatgg
    22561 atcctcgacg agccttttac cgcgattgac gttaacggcg tcgatcgtct gacccagcgt
    22621 atggcgcagc atacggagca gggggggatt gtgattctga ctacccacca gccgctcaac
    22681 gttgctgaaa gtaaaattcg ccgcatttca ctgacgcaaa cgagggccgc atgatgttct
    22741 ggcgcatttt ccgtcttgag ctgcgtgtag cgtttcgcca tagcgccgaa atcgccaacc
    22801 cgctgtggtt cttcctgatt gtaattaccc tttttccgct cagtatcggt ccggagccgc
    22861 aactgctggc gcgtattgca ccgggcatta tctgggttgc tgcgctgctt tcatccttgc
    22921 tggcgctgga acgactgttc cgtgacgatt tgcaggacgg cagtcttgaa caattgatgt
    22981 tgttgccgtt acccttgccc gccgttgtgc tggcgaaggt gatggcgcac tggatggtaa
    23041 ccggtctgcc gttactcatc ctttcgccac tggtagcaat gctactggga atggatgttt
    23101 atggctggca agtgatggcg ctgacgctgc tgctgggaac gcctacgctt ggctttctcg
    23161 gtgcaccggg tgtggcgttg acagtgggac ttaagcgcgg tggtgtgctg ctcagcatac
    23221 tggtgttacc gctgactatc ccattactca tctttgccac cgccgcgatg gacgcggctt
    23281 ccatgcattt gcccgttgac gggtatctgg caattttagg cgcgttgctg gcaggcaccg
    23341 cgacattaag tccttttgcg acggcggcag cgttacgaat cagcattcaa taacgctgga
    23401 ttactgattt ttgttcgagt ctggtatcga aactatgtgg aaaacactgc atcaactggc
    23461 gatcccacca cggctgtatc aaatctgtgg ctggtttata ccgtggctgg caattgccag
    23521 tgtggtcgtg cttaccgtcg gctggatctg gggattcggc tttgctccgg ctgattatca
    23581 gcagggaaat agctaccgca ttatctacct gcatgtgcct gcggcgatct ggtcgatggg
    23641 tatttatgca tcaatggcgg tggcggcgtt tattggcctt gtctggcaga tgaaaatggc
    23701 caacctggcg gtggcggcga tggcccccat tggtgccgtg tttaccttta ttgccctggt
    23761 taccggctct gcatggggaa aaccgatgtg gggcacctgg tgggtatggg atgcacgtct
    23821 gacttctgaa ctggtgctgc tgtttttgta tgtgggtgtg attgccctgt ggcacgcctt
    23881 cgacgaccgc cgtctggcgg gccgtgcggc aggtatcctg gtgctgattg gcgtggtgaa
    23941 tctgccgatt attcattact ccgtggagtg gtggaacacc ctgcatcagg gatcaacgcg
    24001 gatgcagcaa agtatcgatc cggcgatgcg ttcgccgctg cgctggtcga tttttggctt
    24061 cctgctcctg tctgccacgc tgacgctgat gcggatgcgt aatttgattt tgctgatgga
    24121 aaaacgccgt ccgtgggtga gtgaactgat actgaaaaga ggccgtaaat gacccttgca
    24181 tttgcttcct ggaatgaatt tttcgcaatg ggcggttacg ccttttttgt ctggctggcg
    24241 gtggtgatga ccgttattcc gctggtggtt ttggtcgtgc actcggtgat gcagcatcgc
    24301 gcaattctgt gtggtgtgga gcaacagcgg gcgcgtgagg cgcgtttacg tgctgcgcaa
    24361 cagcaggagg ctgcatgaat attcgccgta aaaaccgctt gtggattgcc tgtgctgtgt
    24421 tggcaggttt ggcgctgacc atcggtctgg tgctgtatgc gctgcgctcg aatatcgatc
    24481 tcttttatac gccgggggaa attctctacg gcaagcgtga aactcagcaa atgccggaag
    24541 tcggtcagcg tctgcgcgtt ggcgggatgg tgatgccggg tagtgtgcag cgcgatccca
    24601 attcgctgaa agtgaccttc accatttacg acgctgaagg ctcagtggat gtctcttacg
    24661 aaggcatttt gccggatctg ttccgtgaag ggcagggcgt tgtggtgcag ggcgagctgg
    24721 aaaaaggcaa tcatatcctc gcgaaagaag tgctggcgaa acacgacgaa aactacacgc
    24781 cgccagaagt tgagaaagcg atggaagcta accaccgtcg cccggcgagt gtttataagg
    24841 acccagcatc atgatgccgg aaattggaaa cgggctgctg tgcctggcgc tgggaattgc
    24901 gctgctgctg tccgtgtatc cgctatgggg cgtggcgcgc ggagatgcgc gcatgatggc
    24961 gtcttcccgc ttgtttgcct ggatgctgtt tatgtctgtg gctggcgcat ttctggtgct
    25021 ggtcaatgct ttcgtggtca acgactttac cgtcacctat gttgccagca actccaatac
    25081 ccagcttccg gtgtggtatc gcgtggcggc tacctggggc gcgcatgaag gttcgcttct
    25141 gctgtgggtg ctgctgatga gcggctggac ctttgcggtg gcgattttta gtcagcgtat
    25201 tccgctggat attgtggccc gtgtactggc gataatgggg atggtcagcg tcggcttttt
    25261 gctgttcatt ctctttacct ctaacccgtt ctcacgcacg ctgccgaact tcccaattga
    25321 agggcgcgat cttaacccgc tgttacagga tccggggctg atcttccatc cgcctctgct
    25381 ttatatgggg tacgtgggtt tctcggtggc gtttgctttt gccattgcca ttgcttcttt
    25441 gctgagcggg cgtctggaca gcacttatgc gcgttttact cgcccgtgga cgctggcggc
    25501 gtggattttc ctgacgctcg gcattgtgct cggttccgca tgggcctatt acgaactcgg
    25561 ctgggggggc tggtggttct gggatccggt agaaaacgcc tcgtttatgc cgtggctggt
    25621 ggggactgcg ctgatgcact cactggcggt cactgaacaa cgcgccagct tcaaagcgtg
    25681 gacattactg ctggcaatca gtgccttctc gttgtgtctg ctggggactt tcctggtgcg
    25741 ttccggcgtg ctggtatcgg tacacgcgtt tgcctctgat ccggcacgcg gtatgtttat
    25801 cctcgccttt atggtactgg tgattggtgg ttcgctgctg ctgtttgccg cgcgtggaca
    25861 caaagttcgc tcacgcgtaa acaatgcgct gtggtcgcgg gaatctctgc tgttagcgaa
    25921 caacgttttg ttggtcgccg cgatgctggt ggtattactg gggacgctgc tgccgctggt
    25981 gcacaagcaa ctgggactgg gcagtatttc gattggcgaa ccgttcttca acaccatgtt
    26041 tacctggctg atggtgccgt ttgcgctgct gcttggtgtc ggtcctctgg tgcgctgggg
    26101 gcgcgatcgc ccacgtaaaa tccgcaattt attgattatc gccttcatct caacgctggt
    26161 gctgtcgctg ctgctgccgt ggctgttcga aagcaaagtt gtggcgatga cggtgctcgg
    26221 cctggcaatg gcctgctgga ttgctgtgct ggcaattgcg gaagctgcgc tgcgtatttc
    26281 acgtggcacg aaaaccacct tcagttattg gggaatggtg gcggctcacc tggggctggc
    26341 agtgacaatt gttggtattg cctttagcca gaactatagc gttgagcgtg atgtgcgcat
    26401 gaagtccggc gatagcgtcg atattcatga atatcgcttc accttccgtg atgtcaaaga
    26461 ggtgactggc ccgaactggc gcggcggtgt ggcgactatt ggcgtaacgc gagagggtaa
    26521 accggaaacg gtgctgtatg cggaaaaacg ttattacaac actgccgggt cgatgatgac
    26581 cgaagcggcg attgacggcg gcatcacgcg tgacctgtac gccgcgctcg gtgaagagct
    26641 ggaaaacggc gcgtgggccg tgcgtcttta ctacaaaccg tttgttcgct ggatttgggc
    26701 gggcgggctg atgatggcgt tgggcggact gctgtgtctg tttgatcctc gctatcgtaa
    26761 gcgcgtgagt ccgcaaaaaa ctgcgccgga ggctgtatga agcgcaaagt attgttaatt
    26821 ccgttgatta tcttcctggc gattgccgcg gcgctgctgt ggcagctggc gcgtaatgcc
    26881 gaaggggatg atccgaccaa tctggaatcg gcgctcattg gcaagccagt gccgaagttt
    26941 cgcctcgaat cactggacaa tccggggcag ttttatcagg cggatgtgct gactcagggc
    27001 aaacctgtgc tgcttaacgt ttgggctacc tggtgtccga cctgccgtgc ggaacatcaa
    27061 tatctgaatc agctttctgc gcagggcatt cgcgtggtcg gcatgaacta taaagacgat
    27121 cgccagaagg caataagctg gctgaaagag ctgggcaatc cttacgcgct aagcctgttt
    27181 gatggcgatg gcatgttagg gctggatctc ggtgtctatg gcgcgccaga aacgttcctc
    27241 atcgacggca acggcatcat tcgctatcgc catgcgggcg atctcaatcc tcgcgtctgg
    27301 gaagaagaga tcaagccgct gtgggagaaa tacagtaagg aggccgcaca atgaggtttt
    27361 tattgggcgt gctgatgctg atgatctccg gctcagcgct ggcgaccatc gacgtgttgc
    27421 agtttaagga tgaagcgcag gagcagcagt tccgccaact cactgaagaa ctacgctgcc
    27481 cgaaatgcca gaacaacagc attgccgatt ccaactcgat gattgccacc gacctgcgcc
    27541 agaaagtgta tgaactgatg caggaaggta aaagtaaaaa agagattgtc gattatatgg
    27601 tggcgcgtta cggcaacttc gtcacttacg atccgccgtt aacgccgctg accgtgctgc
    27661 tgtgggtgct tccggtagtg gctattggca ttggcggttg ggtcatttac gcccgttcgc
    27721 ggcgtcgggt acgcgtggtg ccggacgcgt ttcctgaaca aagcgtgccg gaaggtaagc
    27781 gtgccggata tattgtttat ctgccgggta ttgtggtggc gttaattgtg gctggcgtca
    27841 gctactacca gactggcaat tatcagcagg tgaaaatctg gcagcaggcc acggcacagg
    27901 ctccggcgtt gctggacagg gcgctggatc cgaaagccga tccgctcaac gaagaagaga
    27961 tgtcgcgtct tgcgctgggg atgcgtactc aactgcaaaa aaatccggga gatatagaag
    28021 gctggattat gttgggccgc gttggcatgg cgctgggtaa cgccagtatc gccaccgatg
    28081 catacgctac tgcgtatcgc ctcgatccga aaaacagtga tgccgcactg ggttatgctg
    28141 aagcgttgac acgttcatct gatcccaacg acaaccgcct cggtggtgaa ctgctgcgcc
    28201 agttggtgag aacggaccat agcaatatcc gtgtgttaag catgtatgcg tttaatgcct
    28261 ttgagcagca gcgatttggc gaagccgttg ccgcgtggga gatgatgttg aaactcttat
    28321 ctgccaacga tactcgccgt gcggtgattg aacgtagtat cgcgcaggcg atgcaacatt
    28381 tgtcgccgca ggagagtaaa taaaaagagg cccgatggtg atgccatcgg gctattttta
    28441 ttgtgccccg cgttgttgca ggaacagaat ggtggctgcc acgcgtgagc ggacattgag
    28501 tttgcgcagc agattgcgaa tatgtacttt tactgtctgc tctgaaatat tcaacaccga
    28561 ggcaatctgt ttatttgaca gcccctgtgc cagctcgtgc agaacatcca gctcgcgctc
    28621 cgtcagcacg ctgaagggat cttcttccgc gccaaacatt tcacgttcac gtaagtactg
    28681 attgacgcgt tcgctaaaga ctttgctgcc tttcgctcca gcacgaattg cttccagcaa
    28741 tacttccggg tcgctgtctt tcaacagata accgtctgcg cctgcgtcta tcagcgcaaa
    28801 gacatcgctg gaggcatctg atacggtcag gataataatt tgcgcggtaa cgccatccct
    28861 gcgcaaggca ttgagggtat ccaggccact catacctttc atattgagat ccagcaagat
    28921 cacgtcgata tccagtctat ttgccagatc gatcgcgctc gcgccgtcgc ccgcttcggc
    28981 gaccacttca aagctaggat caagctccag taactgacga acaccgcgtc gcataagtgg
    29041 atgatcatcc acaatcatca cctgaaaagg cgttgcttca ggcatagtag tctcctgggg
    29101 ttttattaga atcattatgt ttgagcgtag cgttggtaac gctaccaggc gcgtgaatgt
    29161 taccccattt tatgtatgga catgtagtga aaactacaac cttgtcgttc gctttggcat
    29221 cattttcatt ataaagacgg aattttgcat cagcttc
//
LOCUS       DAJTMC010000032        29057 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.032, whole genome shotgun sequence.
ACCESSION   DAJTMC010000032 DAJTMC010000000
VERSION     DAJTMC010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29057
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.032"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            162..605
                     /locus_tag="OO130_001274"
     CDS             162..605
                     /locus_tag="OO130_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004997737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5364762.1"
                     /translation="MDLALVTGQREGDLAAMRWEDIRDGRLYVEQQKTGAKIRISLPT
                     TISRLNLTLTDVLDNLKKINGKNEKLLGGKTARTIAAQFRIARDTSGLKWEGDPPPFH
                     EIRSLSGRLHSAEKGSDFTQALLGHRSSSMTDKYRDGRGREWKDI"
     gene            841..1659
                     /gene="ybhA"
                     /locus_tag="OO130_001275"
     CDS             841..1659
                     /gene="ybhA"
                     /locus_tag="OO130_001275"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HCR5364763.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETAQQVNAVWKFAL
                     THDDMPQLQHFGKHVEHELGLECEWSWHDQVDIARGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            complement(1660..2718)
                     /gene="modC"
                     /locus_tag="OO130_001276"
     CDS             complement(1660..2718)
                     /gene="modC"
                     /locus_tag="OO130_001276"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HCR5364764.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMA
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(2721..3410)
                     /gene="modB"
                     /locus_tag="OO130_001277"
     CDS             complement(2721..3410)
                     /gene="modB"
                     /locus_tag="OO130_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HCR5364765.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(3410..4183)
                     /gene="modA"
                     /locus_tag="OO130_001278"
     CDS             complement(3410..4183)
                     /gene="modA"
                     /locus_tag="OO130_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5364766.1"
                     /translation="MARKWLNLFAGAALSFAVAGNTLADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTASRQ
                     TLLGNSLVVVAPKASEQKDFIIDSTTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVVSKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(4349..4498)
                     /gene="acrZ"
                     /locus_tag="OO130_001279"
     CDS             complement(4349..4498)
                     /gene="acrZ"
                     /locus_tag="OO130_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HCR5364767.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            4627..5415
                     /gene="modE"
                     /locus_tag="OO130_001280"
     CDS             4627..5415
                     /gene="modE"
                     /locus_tag="OO130_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HCR5364768.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNANNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            5483..6955
                     /gene="modF"
                     /locus_tag="OO130_001281"
     CDS             5483..6955
                     /gene="modF"
                     /locus_tag="OO130_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HCR5364769.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKILLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLVTGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQHLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            7216..8232
                     /gene="galE"
                     /locus_tag="OO130_001282"
     CDS             7216..8232
                     /gene="galE"
                     /locus_tag="OO130_001282"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308814.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HCR5364770.1"
                     /translation="MRVLLTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGIGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            8242..9288
                     /gene="galT"
                     /locus_tag="OO130_001283"
     CDS             8242..9288
                     /gene="galT"
                     /locus_tag="OO130_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HCR5364771.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNHDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            9292..10440
                     /gene="galK"
                     /locus_tag="OO130_001284"
     CDS             9292..10440
                     /gene="galK"
                     /locus_tag="OO130_001284"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HCR5364772.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            10434..11474
                     /gene="galM"
                     /locus_tag="OO130_001285"
     CDS             10434..11474
                     /gene="galM"
                     /locus_tag="OO130_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HCR5364773.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            11676..12428
                     /gene="gpmA"
                     /locus_tag="OO130_001286"
     CDS             11676..12428
                     /gene="gpmA"
                     /locus_tag="OO130_001286"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HCR5364774.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(12586..13638)
                     /gene="aroG"
                     /locus_tag="OO130_001287"
     CDS             complement(12586..13638)
                     /gene="aroG"
                     /locus_tag="OO130_001287"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HCR5364775.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLESGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            13954..14334
                     /locus_tag="OO130_001288"
     CDS             13954..14334
                     /locus_tag="OO130_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HCR5364776.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAATDAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            14448..15389
                     /gene="zitB"
                     /locus_tag="OO130_001289"
     CDS             14448..15389
                     /gene="zitB"
                     /locus_tag="OO130_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HCR5364777.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSIVVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHAHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(15386..16105)
                     /gene="pnuC"
                     /locus_tag="OO130_001290"
     CDS             complement(15386..16105)
                     /gene="pnuC"
                     /locus_tag="OO130_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HCR5364778.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMLLMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSCALSH"
     gene            complement(16143..17186)
                     /gene="nadA"
                     /locus_tag="OO130_001291"
     CDS             complement(16143..17186)
                     /gene="nadA"
                     /locus_tag="OO130_001291"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HCR5364779.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQAECSLDLGCPVEEFNAFCDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQAIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            complement(17435..17510)
                     /locus_tag="OO130_001292"
     tRNA            complement(17435..17510)
                     /locus_tag="OO130_001292"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17475..17477),aa:Lys,seq:ttt)
     gene            complement(17657..17732)
                     /locus_tag="OO130_001293"
     tRNA            complement(17657..17732)
                     /locus_tag="OO130_001293"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17697..17699),aa:Lys,seq:ttt)
     gene            complement(17736..17811)
                     /locus_tag="OO130_001294"
     tRNA            complement(17736..17811)
                     /locus_tag="OO130_001294"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17776..17778),aa:Val,seq:tac)
     gene            complement(17863..17938)
                     /locus_tag="OO130_001295"
     tRNA            complement(17863..17938)
                     /locus_tag="OO130_001295"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17903..17905),aa:Lys,seq:ttt)
     gene            complement(17941..18016)
                     /locus_tag="OO130_001296"
     tRNA            complement(17941..18016)
                     /locus_tag="OO130_001296"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17981..17983),aa:Val,seq:tac)
     gene            complement(18052..18127)
                     /locus_tag="OO130_001297"
     tRNA            complement(18052..18127)
                     /locus_tag="OO130_001297"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18092..18094),aa:Lys,seq:ttt)
     gene            complement(18292..19083)
                     /gene="cpoB"
                     /locus_tag="OO130_001298"
     CDS             complement(18292..19083)
                     /gene="cpoB"
                     /locus_tag="OO130_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HCR5364780.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(19093..19614)
                     /gene="pal"
                     /locus_tag="OO130_001299"
     CDS             complement(19093..19614)
                     /gene="pal"
                     /locus_tag="OO130_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HCR5364781.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(19649..20941)
                     /gene="tolB"
                     /locus_tag="OO130_001300"
     CDS             complement(19649..20941)
                     /gene="tolB"
                     /locus_tag="OO130_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HCR5364782.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPSAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLVFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(21074..22255)
                     /gene="tolA"
                     /locus_tag="OO130_001301"
     CDS             complement(21074..22255)
                     /gene="tolA"
                     /locus_tag="OO130_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HCR5364783.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAAADAKKKAEAEAAKAAAQKKAEAAAAALKKKAEAAEAAAAEARKKAATEAAEKAK
                     AEAEKKAAAEKAAADKKAAADKKAAEKAAAEKAAADKKAAAEKAAAEADDIFGELSSG
                     KNAPKTGGGAKGNNASPAGSGNTKNNGASGADINNYAGQIKSAIESKFYDASSYAGKT
                     CTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKIPKPPSQAVYEVFKNAPLDFK
                     P"
     gene            complement(22320..22748)
                     /gene="tolR"
                     /locus_tag="OO130_001302"
     CDS             complement(22320..22748)
                     /gene="tolR"
                     /locus_tag="OO130_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HCR5364784.1"
                     /translation="MARARGRGRRELKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(22752..23444)
                     /gene="tolQ"
                     /locus_tag="OO130_001303"
     CDS             complement(22752..23444)
                     /gene="tolQ"
                     /locus_tag="OO130_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HCR5364785.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(23441..23845)
                     /gene="ybgC"
                     /locus_tag="OO130_001304"
     CDS             complement(23441..23845)
                     /gene="ybgC"
                     /locus_tag="OO130_001304"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCR5364786.1"
                     /translation="MNTTLFRWPARVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(23995..24288)
                     /gene="ybgE"
                     /locus_tag="OO130_001305"
     CDS             complement(23995..24288)
                     /gene="ybgE"
                     /locus_tag="OO130_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HCR5364787.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIIGLIFFFF"
     gene            complement(24288..24401)
                     /gene="cydX"
                     /locus_tag="OO130_001306"
     CDS             complement(24288..24401)
                     /gene="cydX"
                     /locus_tag="OO130_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HCR5364788.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(24416..25555)
                     /gene="cydB"
                     /locus_tag="OO130_001307"
     CDS             complement(24416..25555)
                     /gene="cydB"
                     /locus_tag="OO130_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCR5364789.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(25571..27139)
                     /gene="cydA"
                     /locus_tag="OO130_001308"
     CDS             complement(25571..27139)
                     /gene="cydA"
                     /locus_tag="OO130_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HCR5364790.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            complement(27987..>29057)
                     /locus_tag="OO130_001309"
     CDS             complement(27987..>29057)
                     /locus_tag="OO130_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HCR5364791.1"
                     /translation="IEESSDDGDEYDYSPAKEEWVITAANAKPQCDIIHEAWQSRAVI
                     RYDMAVPLNLSERSARQSTGRVGVVLVVTLSHNSRRIDVDINLDNQADDHRLRVLVPT
                     PFNTDSVLADTQFGSLTRPVNDSAMNNWQQEGWKEAPVPVWNMLNYVALQEGRNGMAV
                     FSEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLLLRPGRPSGIKMPVPDSQLRGLLS
                     CRLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMP
                     PVGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEEN
                     QGSNLSGPFLPGQSRTFSYRLA"
BASE COUNT         7341 a         7637 c         7085 g         6994 t
ORIGIN      
        1 acatttttaa aacagctata gcttctggtc ttgccgaaga agatccagct tctgcaacca
       61 ggccattcag aactgaagtg aagcgcagtc gactgttaat tgacgagtat ctgttaatca
      121 ggaaaatagc agacgtccag aacgaatggt tcggcctttg catggatctt gcactggtaa
      181 caggacagcg cgaaggagat ctggcggcta tgagatggga ggatataaga gatggcagac
      241 tatacgtgga acaacaaaaa acaggggcaa aaattcgcat ttctctcccc accacgattt
      301 ccagattgaa tttaacactt accgatgtac tggataactt gaaaaaaata aacggtaaaa
      361 acgaaaagtt actcggaggg aagacagcca ggacaatcgc agcacagttc aggattgccc
      421 gagacacatc cggattgaaa tgggaaggtg acccaccgcc atttcatgaa atccgaagtt
      481 tatccggtcg cctgcactca gcagaaaaag gcagtgattt cactcaggcg ctccttggac
      541 atcgttcatc cagcatgacg gataaatacc gtgacggacg agggagggaa tggaaagaca
      601 tttagaatta atttcgaatg atttcgaaac accacttgta attaattgat tataaaggat
      661 taattcctaa gaggcatttg cttcatgaat gctcctttgc atttagcaac tgactgtaac
      721 agctaaaatt agtcgctttt ggcggtgaat gctgaattaa aacgcagaat tttgtccgat
      781 agtggtatcg gtgtaccatt cgcgcagacg aaaattctta accttaatct ggaatacgcc
      841 atgaccacac gcgtgattgc tctcgactta gacggcacct tattgacccc gaaaaagacc
      901 ctgcttcctt catcgataga agccctggcc cgcgctcgcg aagcaggcta tcaattaatc
      961 atcgtcacag gtcgccatca cgtcgctatt catccttttt atcaggcgct ggcgctggat
     1021 acacctgcta tttgctgtaa tggcacctat ttgtatgatt atcatgcaaa aaccgtgctg
     1081 gaagcggacc caatgcccgt taataaagcc ctgcaactca ttgagatgct gaatgaacac
     1141 cacattcacg gtctgatgta tgtggatgat gcaatggtct atgagcaccc gaccgggcat
     1201 gtcattcgca cgtctaactg ggcgcaaacc ctgccgccgg aacagcgtcc gactttcaca
     1261 caagtcgctt ctctggctga aacggcgcaa caagttaacg ccgtatggaa gttcgccctg
     1321 acgcacgatg acatgccgca attgcagcat tttggtaagc atgtcgaaca tgaactggga
     1381 ctggagtgtg aatggtcctg gcacgatcag gttgatattg cacgcggcgg caacagcaaa
     1441 ggtaaacgtt tgacgaaatg ggttgaggcg caaggctggt cgatggaaaa cgtcgtggcg
     1501 ttcggcgata actttaatga tatcagtatg ctggaggccg ctggtacagg cgtggcgatg
     1561 ggcaacgccg atgacgcggt aaaagcgcgc gccaacattg tgattggtga taacaccacc
     1621 gacagcattg cccagttcat ttatagccac ctgatttaat caggcggtta tcgacacact
     1681 tttaatttgc gcgtacagcc acaggcctgg tttgatcgcc agttcatccc tggcccacgg
     1741 gctgatacgc gcccacagcg ttttaccgcc gacttccagc tccacttcca cctgaccgtt
     1801 gtcgtcataa ctattaacaa cttttgcccg caatacgtta cgaatgctgg tttgctgcgg
     1861 cggttgtaaa accagagaaa catcggaagc ctgaatgcgg atacgtagcg cagcttgcag
     1921 cggttcgtcc agcttattga cccacaaatg ctgatcgccc agcgccagcg cggtcatcgc
     1981 gtaatgcgga tgatgctcaa gcaccgtcac tttcagaatg ctactttgtt gctctttcgg
     2041 cagccacgga ttcatcacgc tactacccca gacttcttcc agtgcgccaa aggctttcac
     2101 ctgaccgttt tccagtacca tcactctgtc tgccagatgg aggatctcat ccagcgaatg
     2161 gctgacatac aacatcggga tgttgatttc ccgcgtcaga cgttgcagat aaggcaacag
     2221 ttcgcgttta cgcggaatat ccagtgacgc cagcggttca tccagcaaca gcaattctgg
     2281 tgccgtcagc aaagcccgcc caatcgctac gcgctgtttt tcgcctccgg acaggctgcc
     2341 tggtaaacgg tcaagcaacg gttcaatgcc taaaagcgcc accagcttat cgaactgatc
     2401 gaccatgctt ttcgccatgc cgtagcgcag attgccacgc actttgtaat gcggaaacag
     2461 ccgcgcatcc tgaaaaacat agccaacgcg acgcttttct ggcgtcaggc agataccttt
     2521 ttcggcatca tttagtaccc gaccattgag gacaatcctc cctttttgcg ggcgcgtcag
     2581 tccactgatg gcgttaatca gcgaagtttt tccggcaccg gagacgccaa agatagcagt
     2641 gatgccattg gcgggcagcg tttcattaat agtcaggcaa tggttgccca acgtctggga
     2701 aaaattcagt tccagcatga ttagcgcccc gcccgttcac ggctgattct ggccagccat
     2761 tctgaaatca acagggagat catcgccagc gcaatagaaa taatgcacag tctcgccgcg
     2821 ccactttccc cgccgggggt ctggatcagg gtatacatgg cagagggaat ggttcgcgtt
     2881 tcgccaggaa tgttcgacac aaaggtgatg gttgcaccaa actcaccgag agaacgagca
     2941 aaagccagta ccgtaccaac aataattccc ggtaaggtca gcggtaacgt gatagtaaag
     3001 aaaacgcgcc agcgcccggc acctagtgtt cttgcggcct gttcgagttt gacatcaacc
     3061 ccttccagcg ccagacgaat tgcccgcacc atcagcggaa acgacatgac ggcggcagcg
     3121 agaaccgcgc cgcgccagct aaaggcgaag gtaataccaa accagtcata cagacgttca
     3181 ccgataaatc cgcgccgtcc catcgaaact aataataagt aaccgacgac cacgggcggt
     3241 aacaccagcg gtagatgcag tacgctgtcg agcagagctt tgcccggaaa cgtgcaacgc
     3301 accagtaacc aggcaaaaaa gatcccaaac ggcaggctaa acagcacagc cagggaagaa
     3361 actttcaggc ttaataaaac tgcctgccat tctggatcgg tcagtatcat tacttggttg
     3421 taaatccgta acgtttaaag atttccgctg cctgcggtcc cttcagataa tcataaaatg
     3481 ctttcactgt cgcattgtta tgcccttcca caacagcaac cggatattcc acttttttat
     3541 gtgaatcttc cgggaaggtg gcaaccactt ttaccccttt gctgacaact gcgtcagaac
     3601 cgtaaacaat gcccaacggc gcttcattgc gttcgaccaa cgccagcgcg ccacgaacat
     3661 cttccgccgg agccaatttc ggggagagcg tatcccatgc gcccagtttt tgcagtgctt
     3721 cttttgcata aatgccagca gggacatgtt ccggatcgcc aaccgcgagg cgaccgccat
     3781 tcagcagcga agtccagtta gttgtgctgt cgatgatgaa atctttctgc tcgctggctt
     3841 tcggtgctac aacgaccagg ctattgccga gcagtgtctg gcgcgaagcc gtatcgatcg
     3901 cttttttatc aaccgcataa tccatccatt tctgatcggc agagataaac agatccgcag
     3961 gcgctcccgc ttcaatctga cgggccagag tagatgacga agcgaaagaa gaaaccacat
     4021 ccacgccttt ttctttttta tactgcgtag caatgtcctg cattgcgtta gtcagtgatg
     4081 cggcggcgaa caccgtgatt ttcccttcat ctgccagtgt attgccagca acagcgaaag
     4141 agagtgccgc cccggcaaac aagttcaacc atttacgagc cattggtaac cccttaatgt
     4201 aacgttatat aggtgacaat ataacggcta actcagaatt ttcccagcag ttattgctaa
     4261 cctttgtgag gtagataaga aaaaatatcg gctggaaaag caggaagttg agaaaaagaa
     4321 atgcccgact aagtgggcat tcagggaatc aatgattttg tccgggctgg tcttttttac
     4381 caacaccaga aaagatgttg aatacttcac caagaccgta aatcagaccc aggatgatgg
     4441 ccatcacgac aggtaccatg attacggcga ataccagact ttttaataac tctaacatgg
     4501 tcaactccag atatagtcat gagactattc taaccgctaa gcacagaaaa gcactcccct
     4561 tttgtgcggt cagctttgcg tggcgttcgt tttccgtcac aataagactt ttgccaggac
     4621 attgttatgc aggccgaaat ccttctcacc cttaagctcc aacaaaaatt attcgccgac
     4681 ccgcgccgga tttcgctact aaaacacatt gcgctttccg gttccattag ccagggagcg
     4741 aaagatgccg gtatcagcta taaaagcgcc tgggatgcca ttaacgagat gaatcagtta
     4801 agcgagcata ttctggttga gcgcgcaaca ggcggtaaag gtggcggcgg cgcagtactg
     4861 acccgctatg gtcagcgact gattcagctc tatgacttac tggcgcaaat ccagcaaaaa
     4921 gcctttgatg tgttaagtga cgatgacgcc ctgccgctga acagcctgct ggccgcgatc
     4981 tcacgttttt cactgcaaac cagcgcccgt aaccagtggt tcggtaccat caccgcccgc
     5041 gatcatgatg acgttcaaca gcatgttgat gtcttactgg ctgacggaaa aacacgcctg
     5101 aaagtcgcaa ttaccgcaca aagcggcgca cgtctggggc tggatgaagg caaagaagtg
     5161 ttgatattgc taaaagcgcc gtgggtaggc attactcagg acgaagcggt cgcacaaaac
     5221 gccaacaacc aattaccggg tattattagt catattgagc gcggcgcaga gcagtgcgaa
     5281 gtattaatgg cgctacccga cgggcaaaca ctgtgcgcca cagtgccggt aaatgaagcg
     5341 acttctcttc agcaagggca gaatgtcacg gcctacttta atgccgacag cgtgattatc
     5401 gccacgctgt gctaagcgtg ttgacaattt gttatgaaac acgtatccct gtcagtaatc
     5461 gctgcacaaa atgggatata aaatgtcatc gttgcaaatt ttgcaaggca cgtttcgtct
     5521 tagcgacaca aaaacgctgc aattgcctca gctaacgtta aacgcgggtg atagttgggc
     5581 gtttgtcggt tcgaatggaa gcgggaaatc ggccctggcc cgcgcgctgg cgggggaact
     5641 tccgcttttg aaaggtgaac ggcaaagcca gttttcccac atcactcgtc tctccttcga
     5701 gcaattgcaa aaactcgtca gcgacgaatg gcagcggaat aacaccgata tgctcggccc
     5761 tggcgaagat gacaccggac gcactacggc tgagatcatt caggatgaag taaaggatgc
     5821 accgcgttgc atgcaactgg cgcagcagtt cggtattacc gccctcctcg accgacgctt
     5881 taaatacctt tccactggcg agacgcgaaa aatcctgctg tgtcaggcgc tgatgtcgga
     5941 gcctgacttg ttgattcttg atgagccgtt cgatggcctg gatgttgcct cacgtcagca
     6001 gctggctgag cgactcgcct cgttacatca gtccggtatt actctggtac tggtgctcaa
     6061 tcgcttcgat gagatcccgg agtttgtcca gtttgctggc gtgctggcgg attgcacgtt
     6121 agcggaaact ggcgctaaag aggaactgct ccaacaagca ctcgtcgcgc aactggcaca
     6181 tagtgaacag cttgaaggtg tgcaactgcc ggagccggat gaaccttcag cacgtcacgc
     6241 cttacccgcc aacgaaccgc gcattgtgct gaacaatggc gtggtttctt ataacgatcg
     6301 ccccattctt aataacctta gctggcaggt gaatccaggt gaacactggc aaattgtcgg
     6361 gccaaatggt gcaggaaaat cgacgttatt aagcctggtt actggcgatc atccgcaagg
     6421 ttacagcaac gatttgacgc ttttcggacg acgtcgcggc agcggcgaaa ccatctggga
     6481 tatcaaaaag catatcggtt acgtcagcag tagtttgcat ctggattacc gggtcagcac
     6541 taccgtgcgt aatgtgattc tttctggcta ttttgattcg attggcattt atcaggccgt
     6601 ttcggatcgc cagcaaaaac tggtgcagca gtggctggat attctcggca ttgataaacg
     6661 tacggctgac gctccgttcc atagtctttc ctggggacag cagcatctgg cgctgatcgt
     6721 ccgcgcactg gtgaaacatc cgacgttgct tattctcgat gaaccactac aggggcttga
     6781 tccgctcaat cgccagctta tccgccgttt tgttgatgtg ctgattagcg aaggtgaaac
     6841 gcaattgttg tttgtttcgc accacgctga agatgcgcct gcctgtatta cccatcgcct
     6901 tgagttcgtg ccggacggtg gactctatcg ctatgtgctg acaaaaatat attgagtcgg
     6961 tagtgctgac cttgccggag gcggcctaag cgccctctcc ggccaacggt tcgacgcatg
     7021 caggcatgaa accgcttctt ttttcagata aaaagcgcaa tcattcataa accctctgtt
     7081 ttataatcac ttaatcgcgc ataaaaaacg gctaaattct tgtgtaaacg attccactaa
     7141 tttattccat gtcacacttt tcgcatcttt gttatgctat ggttatttca taccataagc
     7201 ctaatggagc gaattatgag agttctgctt accggtggta gcggttacat tggaagtcat
     7261 acctgcgtgc aattactgca aaacggtcat gatgtcatca ttcttgataa cctctgtaac
     7321 agtaagcgca gcgtactgcc tgttatcgag cgtttaggcg gcaaacatcc aacgtttgtt
     7381 gaaggcgata ttcgtaacga agcgttgatg accgagatcc tgcacgatca cgctatcgac
     7441 accgtgatcc acttcgccgg gctgaaagcc gttggcgaat cggtacaaaa accgctggaa
     7501 tattacgaca acaatgtcaa cggtactctg cgcctgatta gcgccatgcg cgccgctaac
     7561 gtcaaaaact ttatttttag ctcctccgcc accgtttatg gcgatcagcc caaaattcca
     7621 tacgttgaaa gcttcccgac cggcacaccg caaagccctt acggcaaaag caagctgatg
     7681 gtggaacaga tcctcaccga tctgcaaaaa gcccagccgg actggagcat tgccctgctg
     7741 cgctacttca acccggttgg cgcgcatccg tcgggcgata tgggcgaaga tccgcaaggc
     7801 attccgaata acctgatgcc atacatcgcc caggttgctg taggccgtcg cgactcgctg
     7861 gcgatttttg gtaacgatta tccgaccgaa gatggtactg gcgtacgcga ttacatccac
     7921 gtaatggatc tggcggacgg tcacgtcgtg gcgatggaaa aactggcgaa caagccaggc
     7981 gtacacatct acaacctcgg cgctggcata ggcagcagcg tgctggacgt ggttaatgcc
     8041 ttcagcaaag cctgcggcaa accggttaac tatcattttg caccgcgtcg cgagggcgac
     8101 cttccggcct actgggcgga cgccagcaaa gccgaccgtg aactgaactg gcgcgtaacg
     8161 cgcacactcg atgaaatggc gcaggacacc tggcactggc agtcacgcca tccacaggga
     8221 tatcccgatt aaggaacgac catgacgcaa tttaatcccg ttgatcatcc acatcgccgc
     8281 tacaacccgc tcaccgggca atggattctg gtttcaccgc accgcgctaa gcgcccctgg
     8341 cagggggcgc aggaaacgcc agccaaacag gtgttacctg cgcacgatcc agattgcttc
     8401 ctctgcgcag gtaatgtgcg ggtgacaggc gataaaaacc acgattacac cgggacttac
     8461 gttttcacta atgactttgc ggccttgatg tctgacacgc cagatgcgcc agaaagcaac
     8521 gatcctttga tgcgttgcca gagcgcgcgc ggtaccagcc gggtgatctg cttttcaccg
     8581 gatcacagta aaacgttgcc agaactgagc gttgcggcat tgacggaaat cgtcaaaacc
     8641 tggcaggagc aaaccgcaga gctgggaaaa acgtacccgt gggtgcaggt ctttgaaaac
     8701 aaaggtgcgg cgatgggctg ctctaacccg catccgcacg gacagatttg ggcaaatagc
     8761 ttcctgccta acgaagctga gcgcgaagac cgcctgcaaa aagaatattt tgccgaacag
     8821 aaatcaccaa tgctggtgga ttatgttcag cgcgagctgg cagacggtag ccgtaccgtt
     8881 gtcgaaaccg aacactggtt agccgttgtg ccttactggg ctgcctggcc gttcgaaacg
     8941 ctactgctgc ccaaagccca cgttttgcgg atcaccgatt tgaccgacgc ccagcgcagc
     9001 gatttggcgc tggcgttgaa aaagctgacc agtcgttatg acaacctctt ccagtgctcc
     9061 ttcccctact ctatgggctg gcacggcgca ccatttaatg gcgaagagaa tcaacactgg
     9121 cagctgcacg cgcactttta tccgcctctg ctgcgctccg ccaccgtacg taaatttatg
     9181 gttggttatg aaatgctggc agagacccag cgagacctga ccgcagaaca ggcagcagag
     9241 cgtttgcgcg cagtcagcga tatccatttt cgcgaatccg gagtgtaaga aatgagtctg
     9301 aaagaaaaaa cacaatctct gtttgccaac gcatttggct accctgccac tcacaccatt
     9361 caggcgcctg gccgcgtgaa tttgattggt gaacacacgg actacaacga cggtttcgtt
     9421 ctgccctgcg cgattgatta tcaaaccgtg atcagctgtg caccacgcga tgaccgtaaa
     9481 gttcgcgtga tggcagccga ttatgaaaat cagctcgacg agttttccct caatgcgccc
     9541 attgtcgcgc atgaaaacta tcaatgggcg aactacgttc gtggcgtggt gaaacatctg
     9601 caactgcgta acaacagctt cggcggtgtg gacatggtga tcagcggcaa tgtgccgcag
     9661 ggtgccgggt taagttcttc cgcttcactg gaagtcgcgg tcggaaccgt attgcagcag
     9721 ctttatcatc tgccgctgga cggcgcacaa atcgcgctta acggtcagga agcagaaaac
     9781 cagtttgttg gctgtaactg cgggatcatg gatcagctaa tttccgcact cggcaagaaa
     9841 gatcatgcct tgctgattga ctgtcgttca ctggggacca aagcagtttc catgccgaaa
     9901 ggtgtggctg tcgtcatcat caacagtaac ttcaaacgta ccctggttgg cagcgaatac
     9961 aacacccgtc gtgaacagtg cgaaaccggt gcgcgtttct tccagcagcc agccctgcgc
    10021 gatgtcacca ttgaagagtt caacgctgtt gcacatgagc tggacccaat cgtggcgaaa
    10081 cgcgtgcggc atatcctgac tgaaaacgcc cgcaccgttg aagctgccag cgcgctggag
    10141 cagggcgacc tgaaacgtat gggcgagttg atggcggagt ctcatgcctc tatgcgcgat
    10201 gatttcgaaa tcaccgtgcc gcaaattgac actctggtag aaatcgtcaa agctgtgatt
    10261 ggcgacaaag gtggcgtacg catgaccggc ggcggatttg gcggctgtat cgtcgcgttg
    10321 atcccggaag agctggtgcc tgccgtacag caagctgtcg ctgaacaata tgaagcaaaa
    10381 acaggtatta aagagacttt ttacgtttgt aaaccatcac aaggagcagg acagtgctga
    10441 acgaaactcc cgcactggca cccgatggtc agccgtaccg actgttaact ttgcgtaaca
    10501 acgcagggat ggtagtcacg ctgatggact ggggtgcgac tttactttca gcccgtattc
    10561 cgctttccga tggcagcgtc cgcgaggcgc tgctcggctg tgccagcccg gaatgctatc
    10621 aggatcaggc cgcgtttctg ggggcctcta ttggtcgtta tgccaaccgc atcgccaata
    10681 gccgttatac ctttgacggt gaaaccgtga ccctttcgcc aagtcagggc gttaaccagc
    10741 tgcacggcgg cccggaaggg ttcgacaaac gtcgctggca gattgtgaac cagaacgatc
    10801 gtcaggtgct gtttgccctg agttcagatg atggcgatca gggcttcccg ggtaatctcg
    10861 gcgcaacggt gcaatatcgt ctgaccgacg ataaccgtat ctccattact tatcgcgcca
    10921 cagttgataa accttgcccg gtgaatatga ctaatcacgt ctatttcaat cttgacggcg
    10981 agcagtctga cgtgcgcaat cacaagttgc agattctggc ggacgaatat ctgccggttg
    11041 atgaaggcgg cattccgcac gacggcctga aatctgtcgc cggaacgtct tttgatttcc
    11101 gcagcgccaa aatcatcgcc agtgagtttc ttgccgacga cgatcagcgc aaagtgaaag
    11161 gttacgatca cgcattcttg ttacaggcca aaggcgatgg caagaaagtg gcggcgcatg
    11221 tctggtcagc agatgaaaaa ttgcagctga aggtctacac caccgctccg gctctgcaat
    11281 tctactccgg caacttcctc ggcggcacac cgtcgcgggg aacagaacct tacgccgact
    11341 ggcaagggct ggcgttggaa agcgagtttc tgccggacag cccgaaccac cctgaatggc
    11401 cgcaaccgga ctgcttcctg cgtcctggcg aagagtattc cagcctgacg gaatatcagt
    11461 ttattgctga gtaatgttaa gccctcgtca tgagggcttt atctcatatt gctcaaatca
    11521 ccagcaaaca ccgacatatt tgcaactcaa tattcacaac aaccttacac tgcgccacta
    11581 ttttcgctat ggttatgcgt aagcattgct gttgcttcgt cgcggcaata taatgagaat
    11641 tattatcatt aaaagatgat ttgaggagta agtatatggc tgtaactaag ctggttctgg
    11701 ttcgtcatgg cgaaagtcag tggaacaaag aaaaccgttt caccggttgg tacgacgtgg
    11761 atctgtctga gaaaggcgta agcgaagcaa aagcagcagg taagctgctg aaagaggaag
    11821 gttacagctt tgactttgct tacacttctg tgctgaaacg cgctatccat accctgtgga
    11881 atgtgctgga cgaactggat caggcatggc tgcccgttga gaaatcctgg aaactgaacg
    11941 aacgtcacta cggtgcgttg cagggtctga acaaagcgga aactgctgaa aagtatggcg
    12001 acgagcaggt gaaacagtgg cgtcgtggtt ttgcagtgac tccgccggaa ctgactaaag
    12061 atgatgagcg ttatccgggt cacgatccgc gttacgcgaa actgagcgag aaagaactgc
    12121 cgctgacgga aagcctggcg ctgaccattg accgcgtgat cccttactgg aatgaaacta
    12181 ttctaccgcg tatgaagagc ggtgagcgcg taatcatcgc cgcacacggt aactccctgc
    12241 gtgcgctggt gaaatatctt gataacatga gcgaagaaga gattcttgag ctgaatatcc
    12301 cgactggcgt gccgctggtg tatgagttcg acgagaattt caaaccgctg aaacgctatt
    12361 atctgggtaa tgctgacgag atcgcagcga aagcagcggc ggttgcaaac cagggtaaag
    12421 cgaagtaaac gtcattcgtt taaaatgaga aagccgactg caagtgagtc ggcttttttg
    12481 ttgctaacaa tggagcacat tgcctgatgc gacgcttcgc gacttatcag gcctgtggtg
    12541 attcatcgga tacgccactc tgacggcgca tccgacaatt aaaccttacc cgcgacgcgc
    12601 ttttactgca ttcgccagtt gacgtaacag agcatcggta tcttcccagc cgatgcaggc
    12661 atcggtgatg ctcttaccgt aggccagcgg ctccccgctc tcgaggctct gattgccttc
    12721 caccagatgg ctttccacca tcacgccaat aatggccttt tcgccaccgg caatctgctg
    12781 gcaaacgtca gcacaaacat ccatctgctt tttgaattgt ttggacgagt tagcatggct
    12841 gaaatcgatc atcacctgtg ctggcaggcc tgctttgttc agcccttctt tcacttcagc
    12901 aacgtgcttc gcgctgtagt taggctcttt accgccgcgc agaatgatat ggcaatcgcc
    12961 gttaccgctg gtattcacaa tcgccgaatg cccccatttc gttacggaca ggaagcagtg
    13021 cggcgcaccg gcggcattaa tggcatcgat agccacttta atcgtaccgt cggtgccatt
    13081 tttgaagccg actggacaag aaagccctga tgccagttcg cggtgcacct gcgattcggt
    13141 ggtacgtgcg ccaattgcgc cccagctcat caggtcagcg agatattgtg gggtgatcat
    13201 atcgagaaac tcacccgccg ctggcagacc gctgtcgtta atatcaagca gcaatttacg
    13261 ggctatacga agaccgtcgt tgatctggaa gctgttatcc atatgcggat cgttaatcag
    13321 ccctttccag cccaccgtgg tacgcggctt ttcaaaatag acgcgcatta cgatttccag
    13381 ctcatctttc agctcttcac gcagcgccag caagcgagtg gcatactctt tagccgcgac
    13441 aggatcatga attgagcacg ggccaatcac caccaacagg cgatcatcat tacctttcag
    13501 gatcttatgg atcgcttttc gggcatgggc aaccgtattc gcggcatttt cagtagcggg
    13561 gaatttttcc agcaatgcga caggaggaag taactctttg atttctttga tgcgtaaatc
    13621 gtcgttctga taattcatgt ctgttccagt gttgccatac ttatcttagt gaatgcaata
    13681 cttccaatct atatcttccg tcagaatgtg taaacggggt tttacattat gaacggatta
    13741 atcctggaat tagcaagaaa aacgccagat tgtcgcgaaa aacgagatct ctcctacaat
    13801 ttctaactgt aactcctttg tttgttaatt atttcaagat tctctgctgc gtttcataaa
    13861 ctggctgaaa tcttaaacca atgccttata ttcacctgca aatgcactgt tggaagaggt
    13921 tatccgacat aacgaccata acaggagcat cctatgaaaa tgacaaaact ggccacatta
    13981 tttctgactg ccactctaag ccttgccagc ggtgccgcac tggctgccga tagcggagcg
    14041 caaactaata acggccaggc aaacgccgca actgatgcgg gccaggtagc ccctgacgcc
    14101 cgtgaaaatg tcgcgccaaa taacgtcgac aataacgggg taaataccgg ttctggcggc
    14161 acaatgctgc attcggatgg ttcttcaatg aacaatgacg gaatgaccaa agatgaagag
    14221 cacaagaaca ccatgtgcaa agatggtcga tgtccggata tcaataaaaa ggtacaaacc
    14281 ggtgatggca tcaataatga tgtcgatacc aaaaccgacg gtaccacgca gtaaccctgc
    14341 aaaatagcca ataatgggag agcggagtct ctccctttta atttttcact tctaaaagca
    14401 gtatgatgtc ataaagttaa gtgagaataa ttaagggatg actcgtcatg gcgcactcac
    14461 actcacacac gtcctcacac ctgccagaag ataataatgc tcgtcgcttg ttgtatgctt
    14521 tcggcgtgac tgccgggttt atgctggtag aagtcgttgg tggttttctt tctggttctc
    14581 tggcattgct ggccgatgcg ggtcatatgt tgaccgatac tgccgccctg cttttcgccc
    14641 tactcgccgt gcaattttca cgtcgtcctc ccaccattcg ccacactttc ggttggctaa
    14701 gactgaccac gctcgcggcg tttgtgaatg ccatcgccct cgtggtaatt accattttga
    14761 ttgtctggga ggcgatagaa cgcttccgca cgccgcgtcc ggtcgagggc ggcatgatga
    14821 tggcaattgc cgtggctggg ttgctggcaa atatactttc tttctggtta cttcatcacg
    14881 gcagcgaaga gaaaaacctc aacgtacgag cagcggcatt gcatgtgctc ggggacctgt
    14941 tgggttcggt tggggctatt attgccgcgt tgattattat ctggaccggc tggacacctg
    15001 ctgaccccat tctctcgata gtggtgtcgc ttctggttct gcgtagcgcg tggcgactgt
    15061 tgaaagatag cgtgaatgaa ttacttgaag gtgcaccggt atcgctggat atcgctgaac
    15121 tgaagcgtcg tatgtgccgg gaaatcccgg aagtccgcaa tgtgcaccat gtacatgtat
    15181 ggatggtagg tgagaagccg gtgatgacgc tgcatgtgca ggtgatcccg ccacacgctc
    15241 acgatgcctt gttggatcag atccaacact acctgatgga tcactatcag attgagcacg
    15301 ccaccattca gatggaatat caaccttgtc atgggccaga ctgccacctg aatgaagggg
    15361 tgtctggcca ttcacatcat caccattaat gggacagcgc gcatgagcct ctttcacgtg
    15421 cgctgttgat ccacatccga ctgccgttga gcgcaataaa ggtcaggatg atgtactcca
    15481 gagacatggc gtaaacgccc tgaagtgcaa aaataacgac gctaatcacg ttaataatca
    15541 cccacaacag ccagttttcc acatacttac gcgtcatcag aagcattgcc acgatagata
    15601 acaccatcat gcatgaatcc cagaacggga aagcgtccgg ttgcagttca ggcatcacaa
    15661 cctgtaatcc taatgcttgc atgatcatga ctgccacgcg ggtcaaaaat gcaaacaccg
    15721 gattgataaa taccgtcatc agaccaatcg aaacaacgca aaccgccaac cagctgagcg
    15781 ccttcggcaa tggtaaccag cgaattttca actccgcctc gttctgactg gtttgtcgcg
    15841 accacgcata ccaaccgtaa atattcgcgg caaagaaaaa cacctgtaat agcaggctgg
    15901 catacagctg aatctgaaag aaaataatgc caaacaaggt gacgttgatc aggccaaaga
    15961 agtagttgct gatcttctcc agactggcaa ggccaataca cagcaacccg gcgatcgtgc
    16021 ctaccgcttc gatccatgag agatcataac cgcctgctcc tattggtata tgtaccagga
    16081 tattctgcac actaaaaaaa tccatctttc ccccaaagcg taacgttacg ccttattatt
    16141 cgttatccac gtagtgtagc cgcaaaatcc agcatacgat tgagcggcac cagcgccctc
    16201 tctcgcagcc tttcatcaac atgaacctcg tgattgcttc cttcctgttc taatgcctct
    16261 gcgatggcct gaaggccatt catggccatc cacggacaat gcgcgcagct gcggcaggtt
    16321 gcaccctcac ctgcggttgg tgcttccagt aactctttat ctggcaccgc ctgctgcatt
    16381 ttgtagaaaa taccccgatc ggttgccaca ataagcctct gatgtggcaa tgttttcgca
    16441 gcagcgatca gttgactggt ggaaccgacc gcatccgcca tatcgacaat agcttgtggt
    16501 gattctggat gcaccagtat ggcagcatcc gggtattctt cttgcaagcg ggttaacgcc
    16561 tgagtcttaa attcatcatg cacaatacag gcaccctgcc agcatagaat gtctccaccc
    16621 gtctgttttt gcacgtaacg ccccagatgt ttgtcgggtg cccagatgat tttttcaccc
    16681 aaactatcaa gatgatcaat aagttcgacg gcaatgcttg aagttaccac ccaatctgcg
    16741 cgcgctttta ccgcagcaga agtgttggcg tagacgacga cagtacgatc gggatgggca
    16801 tcgcaaaatg cgttaaattc ttcaacaggg cagccgagat ccagtgaaca ttcagcctga
    16861 agtgtcggca tcagaattgt tttttccgga ctgagaattt tggcggtttc tcccataaat
    16921 ctcaccccag cgactaacaa agtagaagcg ggatgctttg caccgaagcg cgccatttcc
    16981 agagaatcag aaatacagcc accggtttct tctgccagtt gttgaatttc gggatcggta
    17041 tagtagtggg caaccatcac cgcattacgt tcttttagca gacgttttat cttctcgcgg
    17101 taatacgctt tttcatcaat gcttaacggc gtcggcttcg gggggaaagg ataaatcgcc
    17161 gtgtctggat caaacattac gctcatctta ccatctcgtt ttacaggctt aacgttaaaa
    17221 ccgacattag cgtaatattc gctgttatcg gtgtaatttg ttttatatgc taaacaagat
    17281 agtcgaatgg tggggaaaag tcactcgaat tacgcctaat tccaccgacg tagagttgct
    17341 tgcacagtaa gctctgacga tgtcatcagg tttacaaagt gaaattgagg atgcctgaat
    17401 agcaaaaaag cgcctttagg gcgctttttt acattggtgg gtcgtgcagg attcgaacct
    17461 gcgaccaatt gattaaaagt caactgctct accaactgag ctaacgaccc atacgggtgt
    17521 tacctgaaag atttctactc ggggccaatc tttaagattg gtgtgtcgtg caggatgact
    17581 cgcttcgctt gccctacggg ccgttgctgg cgcaacgtta tccttcacgc ttaacatctg
    17641 agtttgatgt taaaattggt gggtcgtgca ggattcgaac ctgcgaccaa ttgattaaaa
    17701 gtcaactgct ctaccaactg agctaacgac ccgagtggtg ggtgatgacg ggatcgaacc
    17761 gccgaccccc tccttgtaag ggaggtgctc tcccagctga gctaatcacc cgatactgcg
    17821 ctggatacta caaactactt ccaccgaacc catcgtaatt agtggtgggt cgtgcaggat
    17881 tcgaacctgc gaccaattga ttaaaagtca actgctctac cgactgagct aacgacccgg
    17941 tggtgggtga tgacgggatc gaaccgccga ccccctcctt gtaagggagg tgctctccca
    18001 gctgagccaa tcacccaata ctacgctgga tactacaact aactatgtta gtggtgggtc
    18061 gtgcaggatt cgaacctgcg accaattgat taaaagtcaa ctgctctacc aactgagcta
    18121 acgacccact ttttcgttgc tttcggtttg tttgatatcc cgtggcaacg gcggcatata
    18181 ttactgattt cagacttgag cgcaacaaaa atttcgatgc agatcactca actgcttatg
    18241 attcgcacga cacgaccaga aataatgcaa cttctggtcg tgtgttatgc attacatcgc
    18301 gttcagacgt ttttgtgcct gtttagcgcc atcagtacca gggtatttac tgataacctg
    18361 ctggtatacg gctttcgctt ttgcggtgtc acctttgtcc tgcatgatga cgccgacttt
    18421 aaacatcgca tcagcagcct ttggtgactt cggatagttt ttcactaccg aagcaaaata
    18481 gtacgccgca tcatcttttt tacccttgtt gtagtttaac tgacccagcc aataattggc
    18541 gtttggcagg taagttgaat cagggtaatt tttgatgaaa ttctgaaatg ccaccattgc
    18601 gtcatcctgg cgggatttat cctgcaccag cgcaatagct gcattgtaat ccgtgtttgc
    18661 atcgccgctt ttcaccggcg cgccagcatt cgcagtacca gcatcagctg tcggcgtcgt
    18721 tgatgccgcc gcaccgcttt gatcgctgct ggttgattgc gccgctgcac caccgctgct
    18781 gaggctgtcg atctgcaaca ggatctgctt ttgccgctcc acgacctgat tcagttgata
    18841 ctgattttcc tgaatctgac cacgcaggga atcaatatcg gattgattat cagaaagttg
    18901 ttgctggagt tgggttaaaa gctggctgtg agcattagaa atacgctcaa gttgagtgac
    18961 gcggtcttcg accgagcctg agccgacact actgattggt gcctgagcaa aagcggccca
    19021 gggggccgct ataccaacca gtaacgacag actcaatagt tgatgtctga agttactgct
    19081 catgcaattc tcttagtaaa ccagtaccgc acgacggttt ttggagtatg ccgcttcgtc
    19141 atgacccagt actgcaggtt tttctttacc gtaagaaacg atggagatct ggtctgcaga
    19201 aacgccttta ccctgcaggt acatcttaac ggcgttcgca cgacgttcac ccagggagat
    19261 gttgtattcc ggagtaccac gttcgtccgc gtgaccttct acggtgactt tgtaagacgg
    19321 gttgctacgc aggaagtttg catgtgcatc cagcatttga gcgaagtcag aacggatatc
    19381 gtacttgtcc agatcgaagt aaacgatgtt gttctgctgc agctgttgca tttgcagacg
    19441 agcctgctct tcggaagaca tgttgccgtt gccgccgttc gcatccatac cagtgccggc
    19501 acccagcatg ccttcgctgc cgtcattgct ggcgttcttg ttggaagaac atgccgcaat
    19561 tgccataaca ggcagagcaa tcatcagccc tttcagcact ttgttcagtt gcatttcaat
    19621 gattccttta ctattcaatt aattattatc acagatacgg cgaccaggca gggaatttga
    19681 cctgtccatc agttgccgga agacgcgctt tgaaacgccc atctgtagaa accaaattca
    19741 gcacggatcc catcccctga gaagagctgt agattaccat agtgccatta ggtgccagac
    19801 ttggcgtttc atccaggaac gtggacgaca gaacttgtac gcctcccgtt gccagatctt
    19861 gtttggcaat gtgctgctgc ccaccattgg agctgaccat taccataaat ttaccgtcgc
    19921 tgctgacatc cgcatcctgg ttctgcgaac cttcccaggt aatacgttgt ggcgcaccgc
    19981 cgttgatatt cactttataa acctgcggac gaccggcctg gtcagaagta aataccaggt
    20041 tctggctatc cgggaaccag gtcggttcgg tattgttact gcgaccatca gtcacctggc
    20101 ggatctgacc agaagccaaa tccattacgt acaggttcag actaccggtt ttcgacaagg
    20161 cgaatgccag tttgctgccg tctggcgaga atgcaggtgc accgttgtga cgcgggaatg
    20221 aagccacctg acgtacagcg ccatttgcca gcgtctgaat aaccagcgcg gaacgaccgc
    20281 tttcgaaggt cacataagcc agtttagaac cgtctggtga ccacgccgga gacatcagcg
    20341 gctgcggtga acggtgaacg acaaactggt tgtaaccgtc atagtcagat acgcgcagtt
    20401 catacgggaa ctgaccgccg ttggtctgaa caacgtaggc aatacgggta cggaacgcac
    20461 ctttaatgcc ggtcagtttt tcaaacactt catcactggc ggtatgacca gcataacgca
    20521 gccactgctt gttcactttg tacgagttct gagcaagtac agtacccggt gcgccgccag
    20581 tgtcaacaag ttgataagca acattgtaag aaccatccgg attcggagtg acctgaccga
    20641 caactacagc gtcaatgccc agtgcggacc atgcagatgg ttgtacttcc tgcgcactac
    20701 ccggctgctg tggcagacga gcgcgatcta acggattaaa tttaccgctg ttacgcaagt
    20761 cagcagcaac gatgccgcca atatcttcag gtgccgcacc aggccccgcc cactggaaag
    20821 gaacaacacc aataggacga ccggaatcta caccgctgtc gatcacaatg cggacttcag
    20881 catgcagaac tgatgcccac agtatgagaa aaccaaatgc tactcgtaat gcctgcttca
    20941 tcatatctcc cttatctgga ccgatggccc acgataattt agcagaatgt taacaaacta
    21001 aaatacacaa aactaccaga accccgtggc aaccggtgcc tgatgttgac cgtccgaaca
    21061 gtcaacatcg cgattacggt ttgaagtcca atggcgcgtt tttgaacact tcatatactg
    21121 cctggcttgg tggtttcggg atcttcgcaa gtttagctgc tgccaacgca gcctgacaaa
    21181 gtgcgggatc gccaccttca ggtttgatat ccagtaacat accatcgggt gccagtttta
    21241 tgcgcagcgt acaggttttg cctgcatagg acgatgcgtc atagaactta ctttcgatag
    21301 cagatttaat ctgcccggca tagttattga tatcggcccc tgatgcgcca ttgtttttag
    21361 tattaccact cccggcaggc gaagcattgt tccctttcgc ccctcccccc gttttcggtg
    21421 cattcttacc agagcttagc tcaccgaaaa tatcatctgc ctctgcggcg gctttttctg
    21481 ccgctgcttt cttatcagct gctgcctttt cagcagccgc tttttctgct gcttttttgt
    21541 cggctgcagc tttcttatca gctgcagcct tttcagcagc cgctttcttc tcagcttctg
    21601 ctttggcttt ttcagcagct tcagttgccg ctttctttct tgcttcagct gcagctgctt
    21661 ctgccgcttc cgctttcttc ttcagtgccg cagcggctgc ctcggctttt ttctgcgctg
    21721 cggctttggc ggcttctgct tctgcttttt tctttgcgtc tgcagccgct ttcttcgctg
    21781 cttcttccgc agctttagca tctgcttcgg ccttcgcttt agcatctgcc gccgctttcg
    21841 ctgccgcctc ttcagcttgc ttctgcttta actcggcctg ttttgcggct tcttcagcct
    21901 gctttttctg ctcctgagcc gctaaccgct ctttctcaag ttgcttcagg cgttcctgtt
    21961 cagccgcttg tttctcacgc agttcttcag cagcctgctg ttccttcatc ttgcgctgtt
    22021 catcagaacg cttcgcgctt gattcctggc tttgcatgcg tttgtactgc tcaactaccg
    22081 cacctgaatc aaccatgaca gcgtcgatgg acgaaccacc gccgcctccg gctgaagctt
    22141 ctatattctc atcgaacgaa ctccagatca gcgccgcaaa taagatgaca tgcagcactg
    22201 ctgaaataat tatcgcccgc ttgagcttgt cgttttgttc ggttgccttt gacactctcg
    22261 gtttccaaaa actgttcgcc tgttacccgc tctctttcaa gcaagggaaa cgcagatgtt
    22321 tagataggct gcgtcattaa accaaccgat ttcacacccg cactatgtaa caagttcagt
    22381 gctttaatta tttcatcgta aggcacatct tttgcgccac cgatcagaaa gaccgttttc
    22441 gggttggcct tgaagcggct ggacacttcc gccaccacct gctctggtgg taaacgctcc
    22501 aggcgatctt tctcaaccac cacggtgtac tgaccaatac cagacacttc aacaatcact
    22561 ggcggattat cgttactgct caccgcctgt gattcagtag cgtctggcag atcgacctcc
    22621 acgctctggg tgatgatggg cgctgtcgcc atgaagatca gcaacagcac cagcagtacg
    22681 tccagcaacg gtacaatgtt gatttcggac ttaagttcgc gacgacctcg tccacgcgct
    22741 ctggccatga cttacccctt attgctctcg ctaacggtaa acgcctggcg gtgcagaatc
    22801 gcggtaaact cttccataaa gttgtcgtaa ttcagttcca gtttgtttac gcgctggttg
    22861 aggcggttgt aggccataac tgccgggata gcggcaaaca gaccaattgc agtcgcaatc
    22921 aacgcttctg cgataccggg cgcaaccatt tgcagtgttg cttgttttac cgccccgagg
    22981 gcgataaagg catgcatgat cccccagacc gtaccaaaca gaccaatata cgggctgatg
    23041 gagccaaccg taccgaggaa cggaatgtgc gtttccagat tttcaagttc acggttcatg
    23101 gagatacgca tagcacgcga cgccccttcc actacggctt ccggcgcatg gctattggca
    23161 cgatgcaggc gcacaaactc tttgaacccg ctgtaaaata tttgttccga accagtcaga
    23221 ttatcccgtt tcccctgact ctcttgatag aggcgagaga gttcgattcc agaccagaat
    23281 ttatcttcaa acgcttcggc ttcgcgcgcc gctgcgttaa gaatacgggt ccgctggata
    23341 ataatggccc aggatgcgat tgaaaaacca atcaaaatca acatgataag tttaaccaga
    23401 aggctagcct tcaggaacaa atcaaggata ttcatgtcag tcactgctta aactccgcga
    23461 caatagactt gggaagcgca cgaggcttca ttttgagtgg gtcaacgcaa acaaccagaa
    23521 cctctgcttc attcagcaaa gtattctcgg cgttgacaat acgttgcgtg aaaaccaaag
    23581 aggtgccacg cattgatgtt atttcagtct gtatttcgag catatcgtcg agccgcgcag
    23641 gtgcgtaata ttccaccgtc attttacgta ccacaaaggc aacgcgttca gccatcagcg
    23701 cctgctgact gaagtgatga tgacgcagca tctctgtgcg tgctctttca taaaaagcga
    23761 cgtaactggc gtggtacacc acaccaccgg catcggtatc ttcatagtag acgcgagccg
    23821 gccatcgaaa cagcgttgta ttcactttac atcccggtaa tgcaacaaaa gttagagctt
    23881 ttaaacgctg ctactatacg cgcgcgattg aggtttggga atgagggcaa gttaagggag
    23941 cgtaaaaatt tatgggcctg gagcggccca taatttttta aagagaattc tgatttaaaa
    24001 gaagaagaaa atcagcccga taatgagaac aatatcggca agcaatgggc aaaaaatccc
    24061 ttgccaaaga accttctgcg gacgaaagcc cacgccgtga atcacaccag cacagacggc
    24121 ccacatcagc aataaaccat gccagatttc cagttcactg gtcttcgcgg caaaacgaga
    24181 tgggtcccaa aacatacatc ctgctaacag aagcgccatc acgaaggaaa gcgcccgcag
    24241 ggggcgcttg tccattaccg catacaaggt cgcgataatc ttactcatca gatgtcttct
    24301 tgaccggctt tgcctgattc gacgtgttca agcgccagcg cggtgattac cccaaacgaa
    24361 caggcaagaa gcgttcccag aatccatgcg aaataccaca tttttagctc cttacttagt
    24421 acagagagtg ggtgttacgt tcaatatctt ctttggtgat acgaccgaac attttccagt
    24481 aacaccaggc ggtgtagagc agaatgatcg gtaccagaac caccgcaacc caggtcatga
    24541 cgttaagcgt cagctggctg gaagttgcat cccacattgt cagacttgcg ttcatcatgg
    24601 tgctggacgg catcacaaac gggaacattg cgataccggc tgtcaggatg atgcaggcca
    24661 gcgtcaggga ggagaacaca aacgcccacg cggctttatc catacgtgca gtcaggatgg
    24721 tcagcagcgg cagaaccaca cccagtgccg gaatagccca cagaattggc gtgttgttga
    24781 agttaaccag ccatgcgcca gcttcacgaa ccacttcttt attcagtggg ttagaggctg
    24841 cgtaatggtc cattgtcgat ttcacgacat aaccatcgat accgtacatc acccatacgc
    24901 cagccagtgc gaaacagacc agtgtcacca gcgcagccac ctgagccgtt gcacgggtac
    24961 gcaggtgcag ttcgcccacg gtacgcattt gcagataggt tgcgccctga gtaatgatca
    25021 tccctacgct caccacgcct gccagcaggc cgaacgggtt aagcaactgg aagaagttac
    25081 cggtgtagta cagacgcaga tattcatcaa cgttgaacgg tacgccctgc aacaggttac
    25141 cgaacgcaac accaattacc agcggcggaa cgaagctacc aatgaagatg ccccagtccc
    25201 acatgttacg ccagcgggtt tcttcaatct tggagcggta gtcaaaaccg accggacgga
    25261 agaacaaaga cgccagcacg aggatcatcg ccacatagaa gccggagaac gcagcggcat
    25321 agaccatcgg ccaggcagca aagagtgcgc cgcccgcggt gatcagccaa acctggttac
    25381 cgtcccagtg tggtgcaatg gagttaatca taattcgacg ctcggtgtcg ttacgaccga
    25441 ggaaacgggt gagcatgccc acccccatgt cgaaaccgtc agtgactgca aaaccaatca
    25501 gcagaacgcc aaccagcagc caccagataa aacgcaatac ttcataatcg atcatttgac
    25561 gactcctgtc ttagcgtgcc ggctgagtag tcgtggaaga ctgctcaaag tgatagcgac
    25621 cggttttcag gctgcttggg ccgaggcgtg caaatttgaa cattaagaac aattctgcca
    25681 ccaggaacag ggtatacagg ccgcaaatca gcaccattga gaagatgaga tcgcctgcgg
    25741 tcagtgacga attcgccaca gctgtcggca gcacttcacc gatagcccac ggttggcggc
    25801 catattcagc cacgaaccag cccgcttcta cagcaatcca cggcagcgga ataccgtaca
    25861 gcgcggcgcg cagcagccat tttttctcgc caatgcggtt gcggatgaca ctccagaaag
    25921 agagcgcgat gattgccagc agcaggaagc cacacgccac cataatacgg aacgcgaagt
    25981 acagcggcgc tacacgcgga atggagtctt tagttgcctg ttgaatctgc gcttcagtcg
    26041 catcagccac gtttggcgta tagcgtttca gcagcagacc gtaaccgagg tctttcttca
    26101 tgctattgaa ctggtcacga accgcctggt cggtagaacc agaacgcagt tgttcgagca
    26161 gagagtacgc cttcatcccg ttacgaatgc gttcttcatg ctgcaccatc agctctttca
    26221 ggccgataac cggggtatcc acggaacgcg ttgcaatgat gcccagtgcg taagggatct
    26281 gaatcgcaaa tttgttcgtc tcctcttcct gatcaggaat gccgaacaga gtaaaggcag
    26341 caggcgcagg ttgcgtttcc cactcggctt caatagcagc cagtttggtt ttctgcacgt
    26401 cgcccatttc gtagccggat tcatcaccca gaacaataac agacagaaca gcagccatac
    26461 cgaagctggc agcgatagca aaggagcgtt tagcgaaggc gaagtcacga cctttcagca
    26521 tataccatgc gctgataccg aggatgaaca tcgcgccagt cacataacca gacgctacag
    26581 tgtgaacgaa tttcacctga gcaaccgggt taagcaccag ctcggagaag ctcaccatct
    26641 ccatacgcat agtttcaaag ttgaaatcgg acgcgattgg gttttgcatc cagccgttcg
    26701 caaccagaat ccacagtgcg gacaggtttg aaccgagcgc caccagccag gtgacacaca
    26761 tatgctgaac tttacccaga cgatcccaac cgaagaagaa cagacctaca aaggtggatt
    26821 cgaggaagaa ggccatcaga ccttcgattg ccagcggcgc accgaagata tcccctacat
    26881 agtgggaata gtaagaccag ttagtcccga actggaactc catggtcaga ccggtagcca
    26941 cacccagagc gaagttgata ccaaacaact tgccccagaa cttggtcata tctttataaa
    27001 tctgtttgcc ggagaggacg tagaccgttt ccataatggc cagcaggaac gccataccga
    27061 gcgtcagtgg cacaaaaagg aagtggtaca tcgcggtcaa ggcaaactgt aagcgcgaca
    27121 gttcgactat atctaacatc atgactcctt gctcatcgca tgaagactcc gagagtgaac
    27181 cccgttagaa agggtcacac gcatgcccca atagaaattt gtttgaacac tttcatcata
    27241 ccatgtgaat gcacacaaaa acggcgtaaa gaaaggttgc aaacatgtta ataaaaactc
    27301 aaattgatcc cacgtatata ttacgccgca aaatccttac aataaacagg tttttgttga
    27361 agcaaatttg catttttcga cggtgatcaa tttatagcta aattaccgcc tttcagccaa
    27421 tttgatcgag aacaatttat ctctttttga tgcccatttc caagacttat acattgataa
    27481 atatcaattc ctacaaatta tgttaatcat gtttcaataa ggtaacttta gtgacattta
    27541 tgtttaaaat gtgtgagtta taaggctgaa attagtttcg gttggcaatt aaagataaaa
    27601 agagacgaag caaaaacagc acggttgacc ttaattcttt ttttggtcac tggctaatac
    27661 gacgcagagt ttcaggatta ctaataaagg cataaatcca cccccgccga tattaacatt
    27721 taattaactt ataattatat tataattaac tcacccaggg ttattatttt gttaaaaata
    27781 aagccacata tacgtggctt gagagtgaag ataaacgcaa aattacctga tgcgctacgc
    27841 ttgccagtgt ctacatcatc tctgcaatat attgaatttg cctgcttttg tagaccgaat
    27901 aaagcgttca tgccgtatcc ggcatgaaca aagcgccctt tgtcaacaat ctggccgcgc
    27961 atatgtggct tcggtttgat tgctattcag gcaagccggt aactgaacgt ccgtgactgg
    28021 ccgggtaaaa aaggccccga tagatttgaa ccttgatttt cctcggtggt aatgtgttca
    28081 tccatcatcg tttctgagca agaaatcacc tcgcgactga aagcaacagt cgcatcacag
    28141 gttgctgatt cagccggatt aaacagccgt aaaatcactt cttgtcggtc ttcagctttc
    28201 ttaagtgcgc ttatcaggca tcccactggg ggcattttca acaaactata actttccggc
    28261 acgttgaatc cggctttgtt gagcttcatc gcatcccatg ggattttgtt gtaacactgt
    28321 actggagtca gccatgctcg cgcctgctga gctacaccag cggcggttgg cgtaccggta
    28381 taactcaata aacttaggcg acaagaaagc agaccacgta gttgtgagtc cgggactggc
    28441 attttaattc ccgaaggccg cccaggcctt aaaagcagat cttctttgcc cagtaagccc
    28501 acgccacgca gcaacgtaat ggcaaaggtt ttcttctctt caccgatgac ttcaaattca
    28561 cgtaaccctt cgctaaagac agccatgccg ttacgccctt cctgtaaggc aacatagttg
    28621 agcatattcc ataccggaac cggcgcttct ttccagcctt cttgctgcca gttgttcatt
    28681 gcactgtcgt tcacggggcg cgttagcgaa ccaaactgcg tatctgccag aacactgtcg
    28741 gtgttaaaag gtgtagggac caggacacga aggcgatgat cgtcagcctg gttatcaagg
    28801 ttgatatcca catcaatacg cctgctgtta tgactaagag tgacaaccaa caccacccct
    28861 actctgccag tggattgccg ggcgctgcgt tctgacaaat tgagcggcac tgccatgtca
    28921 tagcggataa cagccctgct ctgccaggct tcatgaataa tatcgcattg cggtttcgcg
    28981 ttcgctgcgg taattaccca ctcttctttt gcgggtgaat agtcatattc atcaccatca
    29041 tcagagcttt cttcaat
//
LOCUS       DAJTMC010000033        29037 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.033, whole genome shotgun sequence.
ACCESSION   DAJTMC010000033 DAJTMC010000000
VERSION     DAJTMC010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29037
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.033"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            28..1347
                     /gene="potE"
                     /locus_tag="OO130_001310"
     CDS             28..1347
                     /gene="potE"
                     /locus_tag="OO130_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HCR5364792.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYANSWNPHHVPFFSAVGSSIAMTLWAFLGLESACANAEVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPCFELKNKHG"
     gene            1423..1572
                     /locus_tag="OO130_001311"
                     /pseudo
     CDS             1423..1572
                     /locus_tag="OO130_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
     gene            1696..2346
                     /locus_tag="OO130_001312"
     CDS             1696..2346
                     /locus_tag="OO130_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001208812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2625 domain-containing protein"
                     /protein_id="HCR5364793.1"
                     /translation="MREMNQLVNTEDSAWPIIQNWLKDATNHTELLPVNKNLAETALY
                     QLQVTTKSPMGALVYGSGGLLIDNGWLRIAGSGHPRLPRDPVSWTQRPEFAGVRALPI
                     ADDVAGGIFALNGGDLGEDTGCVYYFAPDTLNWESLEVGYSEFLQWALSGDLDTFYEN
                     VRWQQWREDVIKLSATEAFTFYPFLWVQSEEARTRKVISLTELWEMQYQMKETFTQ"
     gene            complement(2387..2881)
                     /locus_tag="OO130_001313"
     CDS             complement(2387..2881)
                     /locus_tag="OO130_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5364794.1"
                     /translation="MKTNRSLVVIVSLITATLLLTACAQPEQSSLAGDWLLTPKDKTR
                     RLTGSIAVNIAPSRCKTNCRGDNLPDNTRRWQLSGGNEKELTYLHNMSAQEKVGLNPG
                     WQCYTSFFMRVCQGKPGTRPIVNEDYVSESGFFGSMMHVGIIELRRCQSENCQQELKA
                     INTH"
     gene            complement(3096..4736)
                     /gene="pgm"
                     /locus_tag="OO130_001314"
     CDS             complement(3096..4736)
                     /gene="pgm"
                     /locus_tag="OO130_001314"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HCR5364795.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALLADGLKGVKRISLDEAMASGHVKEQDLVQPFVEGLADI
                     VDMEAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(4762..5307)
                     /gene="seqA"
                     /locus_tag="OO130_001315"
     CDS             complement(4762..5307)
                     /gene="seqA"
                     /locus_tag="OO130_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HCR5364796.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            5492..6256
                     /gene="ybfF"
                     /locus_tag="OO130_001316"
     CDS             5492..6256
                     /gene="ybfF"
                     /locus_tag="OO130_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR5364797.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDAQQIDKATFIGHSMGGKAVMALTALA
                     RDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPSGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            6396..6689
                     /gene="ybfE"
                     /locus_tag="OO130_001317"
     CDS             6396..6689
                     /gene="ybfE"
                     /locus_tag="OO130_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HCR5364798.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            6829..7359
                     /gene="fldA"
                     /locus_tag="OO130_001318"
     CDS             6829..7359
                     /gene="fldA"
                     /locus_tag="OO130_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HCR5364799.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            7569..7655
                     /gene="uof"
                     /locus_tag="OO130_001319"
     CDS             7569..7655
                     /gene="uof"
                     /locus_tag="OO130_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HCR5364800.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            7648..8094
                     /gene="fur"
                     /locus_tag="OO130_001320"
     CDS             7648..8094
                     /gene="fur"
                     /locus_tag="OO130_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HCR5364801.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(8179..8505)
                     /locus_tag="OO130_001321"
     CDS             complement(8179..8505)
                     /locus_tag="OO130_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5364802.1"
                     /translation="MKKLILIAIMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQHFPDQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(8555..9961)
                     /gene="chiP"
                     /locus_tag="OO130_001322"
     CDS             complement(8555..9961)
                     /gene="chiP"
                     /locus_tag="OO130_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HCR5364803.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAVFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSLGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWVKADGQQGYFLQRMTPAYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFGAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
     gene            complement(10408..12072)
                     /gene="glnS"
                     /locus_tag="OO130_001323"
     CDS             complement(10408..12072)
                     /gene="glnS"
                     /locus_tag="OO130_001323"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HCR5364804.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVESIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTRQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSSEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(12276..14222)
                     /gene="nagE"
                     /locus_tag="OO130_001324"
     CDS             complement(12276..14222)
                     /gene="nagE"
                     /locus_tag="OO130_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HCR5364805.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLNQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPSTAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            14555..15355
                     /gene="nagB"
                     /locus_tag="OO130_001325"
     CDS             14555..15355
                     /gene="nagB"
                     /locus_tag="OO130_001325"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCR5364806.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            15415..16563
                     /gene="nagA"
                     /locus_tag="OO130_001326"
     CDS             15415..16563
                     /gene="nagA"
                     /locus_tag="OO130_001326"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCR5364807.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLARHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            16572..17792
                     /gene="nagC"
                     /locus_tag="OO130_001327"
                     /gene_synonym="nagR"
     CDS             16572..17792
                     /gene="nagC"
                     /locus_tag="OO130_001327"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HCR5364808.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYIGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            17840..18592
                     /gene="nagD"
                     /locus_tag="OO130_001328"
     CDS             17840..18592
                     /gene="nagD"
                     /locus_tag="OO130_001328"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HCR5364809.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETSSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            18989..20653
                     /gene="asnB"
                     /locus_tag="OO130_001329"
     CDS             18989..20653
                     /gene="asnB"
                     /locus_tag="OO130_001329"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HCR5364810.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSNQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            21034..21110
                     /locus_tag="OO130_001330"
     tRNA            21034..21110
                     /locus_tag="OO130_001330"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21068..21070,aa:Met,seq:cat)
     gene            21119..21203
                     /locus_tag="OO130_001331"
     tRNA            21119..21203
                     /locus_tag="OO130_001331"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21153..21155,aa:Leu,seq:tag)
     gene            21227..21301
                     /locus_tag="OO130_001332"
     tRNA            21227..21301
                     /locus_tag="OO130_001332"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21259..21261,aa:Gln,seq:ttg)
     gene            21336..21410
                     /locus_tag="OO130_001333"
     tRNA            21336..21410
                     /locus_tag="OO130_001333"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21368..21370,aa:Gln,seq:ttg)
     gene            21426..21502
                     /locus_tag="OO130_001334"
     tRNA            21426..21502
                     /locus_tag="OO130_001334"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21460..21462,aa:Met,seq:cat)
     gene            21551..21625
                     /locus_tag="OO130_001335"
     tRNA            21551..21625
                     /locus_tag="OO130_001335"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21583..21585,aa:Gln,seq:ctg)
     gene            21663..21737
                     /locus_tag="OO130_001336"
     tRNA            21663..21737
                     /locus_tag="OO130_001336"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21695..21697,aa:Gln,seq:ctg)
     gene            complement(21891..23066)
                     /gene="ubiF"
                     /locus_tag="OO130_001337"
     CDS             complement(21891..23066)
                     /gene="ubiF"
                     /locus_tag="OO130_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HCR5364811.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPGSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            23212..24636
                     /gene="miaB"
                     /locus_tag="OO130_001338"
     CDS             23212..24636
                     /gene="miaB"
                     /locus_tag="OO130_001338"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HCR5364812.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGSTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPSETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            24789..25829
                     /locus_tag="OO130_001339"
     CDS             24789..25829
                     /locus_tag="OO130_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HCR5364813.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLTIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            25826..26293
                     /gene="ybeY"
                     /locus_tag="OO130_001340"
     CDS             25826..26293
                     /gene="ybeY"
                     /locus_tag="OO130_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HCR5364814.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            26383..27261
                     /gene="corC"
                     /locus_tag="OO130_001341"
     CDS             26383..27261
                     /gene="corC"
                     /locus_tag="OO130_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HCR5364815.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            27286..28824
                     /gene="lnt"
                     /locus_tag="OO130_001342"
     CDS             27286..28824
                     /gene="lnt"
                     /locus_tag="OO130_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HCR5364816.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDGDQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSVNGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTHEVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     ARKR"
BASE COUNT         7090 a         7442 c         7397 g         7108 t
ORIGIN      
        1 aagcaccctg ttgaaagggg aaaaattatg agtcaggcta aatcgaacaa aatgggcgtc
       61 gttcagttaa ccatactgac gatggtcaac atgatgggct ccggtatcat catgctgccg
      121 acaaagcttg ccgaagttgg gacaatctca attatctcct ggctggtgac agccgtaggc
      181 tcaatggcac tggcatgggc attcgcaaaa tgcggtatgt tcagccgtaa atcaggcggt
      241 atgggcggtt atgcggagta tgctttcggt aagtccggca attttatggc gaactacacc
      301 tacggtgtct cgctgctgat tgctaacgtc gcgattgcta tttcggcggt tggttacggc
      361 accgaactgc tcggcgcgag tttgtcgcca gtgcagattg gtcttgcgac catcggagtg
      421 ctgtggattt gtaccgtggc taactttggt ggtgcgcgca ttaccgggca aatcagtagc
      481 attactgtgt ggggagtcat tattccggtc gtcggtctgt gcattattgg ctggttctgg
      541 tttagtccga cgctgtacgc taactcatgg aacccgcacc atgtgccgtt ctttagcgct
      601 gtaggttctt ccatcgccat gacgttgtgg gctttcctcg gtctggagtc agcctgtgcc
      661 aacgccgaag tagtggaaaa cccggaacgt aacgtgccga ttgcagttct tggcggtact
      721 ttaggtgcgg cggtgattta tatcgtctcc accaacgtga ttgccgggat tgtgcctaat
      781 atggagctgg caaattcaac ggcaccattt ggcctggctt tcgcacagat gttcacacca
      841 gaagtgggta aagtcattat ggcgctgatg gtgatgtcct gctgcggttc actgcttggc
      901 tggcagttca ccattgccca ggtgtttaaa tcttcatctg atgaaggcta cttccctaaa
      961 attttctccc gtgtaaccaa agtggatgca ccggtgcagg gaatgttgac cattgtgatt
     1021 attcagagtg gattggcact gatgaccatt agcccgtcac tgaacagcca gttcaacgtg
     1081 ctggttaacc tggccgtggt gaccaatatc attccgtata ttctgtcaat ggcggcatta
     1141 gtcattattc agaaggtcgc taatgtgccc ccctcaaaag cgaaagtagc aaactttgtt
     1201 gcctttgttg gtgcgatgta cagtttctat gcgctgtact catccgggga agaagccatg
     1261 ctgtacggtt ctatcgtgac cttccttggt tggacactgt atggtctggt atcaccatgc
     1321 tttgaactga aaaataaaca cggttaataa aacaaagtaa aaaagggcga ttgaatgatc
     1381 gccctttctg tttgttggca aatagttcgt tatttatgcc gcatccggca gatgtagggt
     1441 ctaaacgcga tcgaacacag caactatttg cgtaattata tggggcatct gcgacaaaaa
     1501 ctggaacagg atccggcccg gcctcgccat tttattactg aaaccggtat tgggtatcgg
     1561 tttatgcttt gaaaaaagtg gcaatgtatg gataaagtca ttttttattc aaatataagg
     1621 aacttaatat ttaaaaaatg ttccatacaa ttcctcatca taaatgacac cgtagcacac
     1681 aaggagcaag tagcgatgcg tgaaatgaat cagttagtaa atactgaaga ttcagcctgg
     1741 ccaataatcc agaactggtt aaaagacgcc acaaaccata ccgaactctt accggttaat
     1801 aaaaatttag ccgaaaccgc actttatcaa ttacaggtga caacaaaatc accgatgggt
     1861 gcgcttgtct atggctccgg aggtctgctg attgataatg gttggttacg catagcagga
     1921 tctggtcacc cccggttgcc acgagatcca gtaagttgga cacaacgccc tgaattcgca
     1981 ggcgtgcggg ctttacccat cgctgacgat gtcgcagggg gaatttttgc tcttaatggt
     2041 ggcgatcttg gggaagatac tggctgcgtt tattattttg cgccagatac gctgaattgg
     2101 gaaagtctgg aagttggata ttctgagttt cttcagtggg cattatccgg tgatttagat
     2161 accttctatg aaaatgtacg atggcagcaa tggcgagaag acgtaataaa attatcggca
     2221 acggaagcct tcacgttcta ccctttctta tgggtacaaa gtgaagaagc aagaacaaga
     2281 aaagttattt cgcttactga actctgggaa atgcagtatc aaatgaaaga aacattcacg
     2341 caataagaca tgttcaggtc cgggtttcat tcccggactc actattttaa tgtgtattta
     2401 tcgctttcaa ttcttgctgg caattttctg actggcagcg tcgtaactca ataatcccga
     2461 catgcatcat cgagccaaaa aatccgcttt cgctgacata atcctcattg actatcggtc
     2521 gagtgccggg cttgccctgg caaacccgca taaaaaatga ggtgtagcat tgccaaccgg
     2581 gatttaaacc gaccttttct tgtgctgaca tattatgaag ataagtaagt tctttttcgt
     2641 tcccgcctga caactgccag cggcgggtat tatcaggtaa gttatcacca cggcaatttg
     2701 tcttacaccg ggaaggtgca atatttaccg caatgctacc cgttaatctt ctggttttat
     2761 ctttaggggt cagtaaccag tcgccagcta atgatgattg ttcaggttgg gcgcatgcag
     2821 tcagcagtaa tgtggcggta attagtgaca ctatgacgac cagactcctg ttggtcttca
     2881 ttgggatttc cttgagctac tcagtttgtt gacaaagtgt tcgttgtttt tgccggatgc
     2941 ggcgtgaacg ccttatccgg cctacaaaat ctcgtaaatt caatatattg caccatcctc
     3001 gtaggcctga taagcgtagc gcatcaggca attctgtgtt tgtcatacgt aaaaaagggc
     3061 gatcttccga ccgccctttt tcttatttaa tgtgtttacg cgtttttcag aacttcgcta
     3121 acaatctcaa ccgcttcttt ctcaatctgc ttgcgatgtt cttcaccgag gaagctttcg
     3181 cagtagatct tatatgcgtc ttccgtgcct gacggacgcg cggcgaacca gccgttgtca
     3241 gtcatcactt tcagaccgcc gatagaagca ccattgcccg gtgccgccgt caggcgcgcg
     3301 gtgatcgggt cgcctgccag ggtgctggcg ctcaccattt ccggagacag cttagacagc
     3361 gccgcttttt gtgcggaagt cgcagctgcc tgcaaacggt tgtagctcgg cgcaccaaag
     3421 cgttttgcca gttcgttgta gtgttcctgc gggttcttac cggtgacagc ggtgatttcc
     3481 gccgccagca gacacatgat gatgccgtct ttgtcggtgg accacggcgt gccgtcgaaa
     3541 cgcaggaagg aagcccctgc gctctcttcg ccgccaaagc cgaagctgcc gtcgaacaga
     3601 ccatcaacaa accatttgaa acctaccggg acttctacca gcttgcggcc caagtcattg
     3661 accacacggt cgatcatcgc agaagaaacc agcgttttac cgacagcaac atctttgccc
     3721 cactgcggac gatgctggaa caggtagttg atcgccaccg ccaggtagtg gttcggattc
     3781 atcaaacctg ccggagtgac gataccgtga cggtcataat ccgggtcgtt agcaaacgcc
     3841 agatcgaact tatcacgcag tgccagcagg cccgccatcg cacactcgga ggagcagtcc
     3901 atacggatcg cgccgtcttt atcaaggtgc ataaagcgga aggtttgatc gacctgatcg
     3961 ttaacgatgg tcaggttgag gttgtaatac tcgccaatac gcttccagta ttcgataccg
     4021 gaaccgccca gcggatcaac gcccagcgtc aggcccgctt tctgaatcgc ctccatatca
     4081 acgatatcgg ccagaccttc cacaaacggc tgcaccagat cctgctcttt cacatgaccg
     4141 gatgccatcg cttcgtcgag ggagatacgc ttcacgcctt tcaggccatc ggccagcagt
     4201 gcgttggccc tgtcttccac cactttggtg acgttggtat cagccgggcc accatttggc
     4261 ggattgtact tgataccacc atcttccggc gggttatggg acggtgtaat cacgataccg
     4321 tctgccagcg gaccaccttt tttattgtga accaggatgg cattggaaac ggcaggcgtt
     4381 ggagtgaagc cattgttttc ctgcacaatg acatcaacgc cgttcgctgc cagcacttcc
     4441 agtacggaaa tgaacgcagg ctcggacagg gcgtgagtat ctttacccac atagcaaggg
     4501 ccagtgatgc cgtttttcgc acgttcttca gcaattgcct gagcgatcgc cagaatgtgc
     4561 ggctcgttaa agctgtggcg cgctgcactg ccacggtgac cggaagtacc gaatttcacc
     4621 gcgtgctccg cattccctgc ttctggtttc agtacataat attgcgccgt cagttgggcg
     4681 acgttaatca aatcactctg ttgtgcaggt tggcctgcac gattgtggat tgccattgct
     4741 ttgtcctttg tctgcaacgt tttagatagt tccgcaaacc ttctcaatca attccgccgg
     4801 gaattgcatc gactgcatga tgtgttcgat catgctgcat ttacggccgg tgttggtgtt
     4861 ggtgatcacc caatacggcg tgcctggcac atgtttcggc ttggtctgat taccattttt
     4921 cagcagcgtt tgttcatctg ccgcaaagta aacgcgtgta cgaccgtgca acgattccgt
     4981 tgcttcggca aacgcctggg cgtcaagaga atatagtgta gacaacagca gcataaagcg
     5041 attgaccgct cgcttttgct ctgcgtattc gtcagaaagc agaagttcac gcattgcgcg
     5101 aaccttgtct ttaatcgttt tgaccggctt cgcttcgacg atagcaggtg acgcaacgcg
     5161 aacctctttc gtcaccggag cagcaggctg tgatgcggcg gaaaatttca acatacgccg
     5221 taaaatgtcg gatgcgctct cgccgatatg cttagtgtgg ctggcaatat agctgtagag
     5281 ttcatcatca acttcaatcg ttttcatctt aatccagtgc agtgtcttag ctgaatacaa
     5341 gtcgccaaga gtatatgaat aaatcccggc agcggatagc gtcaagcctg gcttgcagaa
     5401 aaactcggaa taaaccttat ttgttgcagc cctgtggcac aatggtcaac ataataccct
     5461 aacccgacaa cgcgaaaaaa agaactttgc catgaaattg aatatccgcg cgcaaactgc
     5521 acaaaaccag cacaataatt ctcccatcgt tcttgtccat ggtctgtttg gtagcctcga
     5581 caaccttggc gtactggctc gcgatctggt aaacgatcac aatatcatcc aggttgatat
     5641 gcgtaaccac ggtctttcac cgagagatcc ggtaatgaat tacccggcga tggcgcagga
     5701 tcttgttgat actctggatg cacagcagat cgacaaagca acatttatcg gtcactccat
     5761 gggcggtaaa gcggtaatgg cacttacggc actggcccgc gatcgcatcg ataaactggt
     5821 ggcgatcgat atcgcaccgg tcgactatca cgtacgccgt catgatgaga ttttcgcggc
     5881 tatcaacgcg gtcagtgaat cggacgcaca aactcgccag caagcagcag caataatgcg
     5941 ccagcatctt aatgaagaag gggtgattca gtttctgttg aaatcttttg ttgacgggga
     6001 gtggcgcttt aacgtgccgg tattgtggga tcagtatccg catattgtgg gttgggagaa
     6061 aatcccggca tgggatcacc ctgccctgtt tatccctagc ggcaattctc cgtatgttag
     6121 cgagcagtac cgtgatgatt tactggcaca atttccacag gcacgggcgc atgtgattgc
     6181 aggcgcgggt cactgggtcc atgctgaaaa accggatgcg gtattacgcg ctatccgtcg
     6241 ctatctcaat gattaacgca actgattaaa aacccagcgt ccgcgtcgct ttccgcgcgt
     6301 gggcgttggc gtgactcatc gcgctgatgt attatggcgc gctatccatc cgggcagagg
     6361 cctggctgat tgtttccccc gaagtcacca agatcatggc caaagaacaa acggaccgta
     6421 cgacattaga tctgttcgcg cacgagcgtc gaccgggacg accgaaaact aatccgcttt
     6481 cgcgcgatga acagctgcgt attaataaac gcaaccagct aaaacgcgac aaagtacgtg
     6541 gccttaagcg tgtcgaactg aagctgaacg cggaagctgt cgaggcgctg aacgagctgg
     6601 cggagtcgcg caatatgagc cgtagcgaac tgatcgaaga gatgttaatg cagcaactgg
     6661 cggcgctgcg tagtcagggc atcgtttaaa tttccacttt catgtagcac agtgtgcagt
     6721 cctgctcgtt tgctgattcc gaaagactgc ctgttctgct atgattgcct ttatccgtgg
     6781 gcaattttcc acccccattt caataagttt caagaggtta tttcactcat ggctatcact
     6841 ggcatctttt tcggcagcga caccggtaat accgaaaata tcgcaaaaat gattcaaaaa
     6901 cagcttggta aagacgttgc cgatgtccat gacattgcaa aaagcagcaa agaagatctg
     6961 gaagcttatg acattctgct gctgggcatc ccaacctggt attatggcga agcgcagtgt
     7021 gactgggatg acttcttccc gactctcgaa gagattgatt tcaacggcaa actggttgcg
     7081 ctgtttggtt gtggtgacca ggaagattac gccgaatatt tctgcgacgc attgggcacc
     7141 atccgcgaca tcattgaacc gcgcggtgca acgatcgttg gtcactggcc aactgcgggc
     7201 tatcatttcg aagcatcaaa aggtctggct gatgacgacc actttgtcgg tctggctatc
     7261 gacgaagacc gtcagccgga actgaccgct gaacgtgtag aaaaatgggt taaacagatt
     7321 tctgaagagt tgcatctcga cgaaattctc aatgcctgat gtgatgcggc gtagactcat
     7381 gtctacgccg tattaataga taatgccaat caaaataatt gctacaaatt tgtaactttt
     7441 gctgttgtac ctgtacaatg tcccggtgtt caagtggcct tgccgttgta aatgtaagct
     7501 gtgccacgtt tttattaaca atatttgcca gggacttgtg gttttcattt aggcgtggca
     7561 attctataat gatacgcatt atctcaagag caaattctgt cacttcttct aatgaagtga
     7621 accgcttagt aacaggacag attccgcatg actgataaca ataccgccct aaagaaagct
     7681 ggcctgaaag taacgcttcc tcgtttaaaa atcctggaag ttcttcagga gccggacaac
     7741 catcacgtca gtgcggaaga tttatacaaa cgtctgatcg atatgggtga agaaattggt
     7801 ctggctacgg tatatcgcgt actgaaccag tttgacgacg ctggtatcgt cacccgccac
     7861 aattttgaag gcggtaaatc cgtatttgaa ctgacacagc aacatcacca cgatcacctg
     7921 atctgcctcg actgcggcaa ggttatcgaa tttagtgatg attccatcga agcgcgtcag
     7981 cgtgaaattg ccgcaaaaca tggcattcgc ctgactaacc acagtctcta tctttacggt
     8041 cactgtgccg aaggcgattg ccgcgaagat gaacatgcgc acgaaggcaa ataagccagc
     8101 ctgaatgaga aaaagccaac ctgcgggttg gcttttttat gcaagggaaa taagaaaagc
     8161 gagagttaca gctctcactt atttgttatt actgcgaatt tcctgccaaa ctttatcaca
     8221 atcggctttc accgcctgat catttccggt ttgcgttgca cgcagacact gctgataatc
     8281 cagtacgcgg acactttcct ggtcgggaaa atgctgatgt tgtttctctt tcttcagcac
     8341 gtttaacacg ctctggcagg cttcaatttt ttctggcgaa ccttgtgcgg tgttgataca
     8401 ggcgctgtat gcctctttca gacgactgtc ttcctgtggc gcggttgatt gcgcacaagc
     8461 taccagcccc gatgccatta tggcgatgag aatcagtttt ttcatagcaa attcctcaaa
     8521 cgtgcggcag gcacaaaacc tgccgcgtcg ggcatcagaa gatggtgaat ggtgcgatta
     8581 ccataaattt cacgtcacgc tcatcctgga agatgttgcc gtaaccaccg ccccagcttg
     8641 ggatgtcgga gtggttgtcg tattcggtga agtgcagttt gaacatcgtg cctttggcgc
     8701 gaccgtcctg aatggtgtag accgcatcca ggctgtatgc agactcttca atagtccggt
     8761 ttttgtcgta gtacgcatcc ggattgctct gccaggtcgc aggtttagca tcccatgcgt
     8821 aaacgtagga agcgccgatg gcgaagcctg gaagattcca gtttttcagg tcatacatcg
     8881 caccgaagaa gaccgctttt tcgccgttag cgttgaagtc ggaacggtta tcccaccaga
     8941 tatccaggcg gccgttggag gaagcgtagg ctggagtcat acgttgcagg aagtatccct
     9001 gctgaccgtc agccttaacc caggtgcctt cgaggcgcaa atccactacg tcagccgccc
     9061 ggtaaccaaa ggtcaacgct tgcagccagg cggtgccgtc ataaaggtcg ttgacgctgc
     9121 gatcgtcaac tttatcgcgc gtaccataga actggtagct ggtggttaac gggctaccgg
     9181 cgatatcaaa tttgtagctg gctttggcaa aatattgatc gatataccct tccgcctgac
     9241 caaatgccgc ttccagtacg aagttatttt tgaagtcgta tttcgcccca agggagtgca
     9301 gataatcaac tttggtggtt ttatcgttct gataaaactc atccatttcc agatgccacg
     9361 gcgctttgta ttcgttggtc cacatgtagg agaaactcaa cgcaccagca tcgccgtaat
     9421 caaaattcgc cccggcttcc gccccctgat aagtacctgg cataaagctc cagtgcggcg
     9481 ctaacagcgt ttgaccagtt ggctgaatgt aacctgccct cgcccaaacc ggaccgtatt
     9541 taaatttggc cgcagcttta tacaggctta taccgctttt gtcgccagac cagtcttcgt
     9601 cataggcttt attacttttt gaaaacgcga tttcgttcgg gtgggagctg tcgccgtttt
     9661 ccgccatttc aatcgccgta aacacagcaa tatcaaggcc gaacatatca gcagcataac
     9721 cggactgaaa atcgaggttg gcattccagg tggagtgaga aaggttggtt ttgtatttgt
     9781 cgccgtcggt aacatcttta cggtcgcgtt cacgctgcca gtaatatata ccgccggtta
     9841 aggttgaatc gtcgatgaat ccttcggcgc gagcctccgg cattgccata aagcccgaca
     9901 ttgctgtaac accggcgata gccagcgcca gcgtactacg tttgccacta aacgtacgca
     9961 tgggttaatc ctctttgacg tataaattgc tgcaccaaag gtgaacagcg aaaaaaataa
    10021 aaattaaaaa agtagcattg agaaaagtct cgctgactac aggaatagac tatttttcgc
    10081 gacgcgaatt atcaatctct ttctgtaacc agaatgtaag aacatgagca accgcacata
    10141 ttttgatgat ttttgttaca aggcttaagt ttgcggttga aaggcttgca gaagaataaa
    10201 agcgtaaatt tccagcaaga tgaaagcggt tacattttat ttaattatgg tttgtgattt
    10261 taaatttatt ttatggataa attgcgaagc ggaattaatt cgcgaagaaa agcagaaaaa
    10321 acgccgcaga gtgcggcgtt tgggattgtt tgctatttct tgccggatgc gacgtaaatg
    10381 cctcacccgg cctggtagaa cttaaaatta ctcgcctact ttcgcccagg tatcacgcag
    10441 cccaacggtg cggttaaata ccggtttttc cgctgtggaa tggcggctgt cgaggcagaa
    10501 gtaaccttca cgctcaaact ggaatgcttt acccgcaacc gcatctttca gcgacggttc
    10561 agcaaagccc tgtttgatca ccagcgattc cgggttaatc accgacagga aatcatccgc
    10621 agcacctggg ttaggcacgc tgaacagacg gtcgtacaga cggatttcaa ccggcagcgc
    10681 atgtgccgcg ctcacccagt gaataacacc tttgacttta cgaccatctg ccggatcttt
    10741 gcttaaggtg tcggcgtcat aagtacagaa gatggtggtg atattgcctt cggcatcttt
    10801 ctccacgcgt tctgccttaa tgacgtaagc attacgcaga cgcacttctt tacccagcac
    10861 cagacgtttg tactgcttgt tagcttcttc gcggaaatcg gcgcgatcga tccaaatctc
    10921 actgctaaac ggcacctggc ggctacccat ttccggtttg ttcggatggt tcggcatggt
    10981 aaccatttcg ccttcgccct gatagttttc gataaccagt ttcaccggat cgataaccgc
    11041 cattgcgcgc ggggcgtttt cgttgagatc ttcacggatg caggattcca gcgacgccat
    11101 ctcaatggtg ttgtcctgcc tggtcacgcc gatgcgtttg cagaactcac gaatagaagc
    11161 agcagtgtaa ccacgacgac gcagaccgga aatggtcggc atacgcgggt catcccagcc
    11221 ttcaacgtgc ttgtcggtca ccagcaggtt cagcttacgc ttggacatca cagtgtattc
    11281 cagattcagg cgcgagaatt cgtactggcg cgggtgaaca ggaatggtga tgttgtccag
    11341 cacccagtcg tacaggcgac ggttgtcctg gaactcaagc gtacacagag agtgcgtaat
    11401 accttccagg gcatcgctga tgcagtgggt gaagtcgtac atcgggtaga tgcaccactt
    11461 gttgccagtc tggtggtgtt cagcaaattt aatgcggtac agcaccggat cgcgcatcac
    11521 gataaacggt gaagccatgt cgattttcgc acgcaggcag gctttacctt cttcaaaacc
    11581 accggcacgc attttttcga acagcgccag gttctcttca acgctgcggt cgcggtacgg
    11641 gctgttttta cccggttgcg tcagggtgcc gcggtattcg cggatctgtt ccggcgtcag
    11701 ttcatcaacg tacgccaggc ctttattgat cagttcgatc gcataggcgt ggagctgatc
    11761 aaaataatcg gaggagtaac ggacgttacc agaccagtga aaacctaacc actctacgtc
    11821 gtttttgatc gactcaacat actcgatatc ttcttttacc gggttagtgt cgtcgaaacg
    11881 caggttgcac tggcctttat agtcctgggc gatcccgaag ttcaggcaga tagatttcgc
    11941 atggccaata tgcagatagc cattcggctc cggcgggaaa cgggtgtgta ctgtggtgtg
    12001 cttaccactg gccagatctt catcgatgat ctgacggata aagttagtcg ggcgggcttc
    12061 tgcctcactc atcgtggatt cctcaaagcg taaacaacgt ataacggcgt atgatcttat
    12121 aagcggaaca ggctgacaac tgttagtttt ttaaaattga taatctaaca aggattatgg
    12181 ggatgattga tgaaaaaaaa aagcggcaga gaagatctcc accgcttgtc attgttggat
    12241 gcgacgctca agcgtcgcat caggcataaa gcagattact ttttgatttc atacagcggt
    12301 gtttgacccg ccacaacatg gccctgagct ttaatgatca agccactgaa atcgtcgata
    12361 ttgctgcaaa ccaccgggct aatcatcgag cgggcgttag cgttcaggta atccagatcc
    12421 atttccagaa tcggttgccc tgcgcttacc tgcgcaccct cttccaccag acgtttaaag
    12481 cctttacctt ccagcgctac ggtgtcgata cccatatgga cgacgatctc cgcgcctttt
    12541 tcggtttcca ggcagaacgc gtggttggtg ttgaagattt tcacgattgt ccctgcggct
    12601 ggtgatacga cgattttatc tgtcggtttc accgccacac cgtcacccac cgctttgctg
    12661 gcgaatgctt cgtcaggaac ctgatccagt gccacgacat caccggtaat cggcgatacc
    12721 agctccgcga tagataccgc gtttggtaca gcctgcggtt ttgctacagg cgcggcagtt
    12781 gacggagttg cttcagctga cgcagcggct accggaccac gggcaacgac tttcttcatc
    12841 gcatcgccga tggattctgc tttcgcgcca acaatcacct gaatagtctg tttgttcagt
    12901 ttcactacac cagaagcacc cagacgttta cacatcgtat cgttgacgcg ggcagagtca
    12961 gccacggtca ggcgcagacg ggtgatacag gcgtcaattg ctttcaggtt gtcagtgccg
    13021 ccaaccgcag caatatagtt ggtcgccagt tgattgaggc cttcttcagt gttgctgttg
    13081 gcttcttcag taacgatctc atcttcttta tcttcacgac ccggcgtttt caggttgaac
    13141 atgcggataa ccaaactgaa caccacgaag tagatagcga agaagacaac acccatcacc
    13201 agcagcatcc agacgttctg gctggcggcc ggcaggttat acatcaacgc gtagtcgata
    13261 gcccccgcag agaaagagaa gccagcatgg atacccagca gtgttgccac aaacaggctg
    13321 ataccggtca gcagtgcgtg caggaggtac agcagcggtg caaggaacat gaacaggaat
    13381 tccagcggct cagtcacacc ggtcaggaac gcagtaacag caacagaaag cagcatcccg
    13441 ccaaccatcg gacgacgctc tttcggtgct gcgaagtaca tcgccagcgc cgcacccggc
    13501 agaccgaaca tcataatcgg gaagaagccg gacatgaaca tccccgcggt gccgtcaccg
    13561 gcatagaagc ggttgatatc accgtggaaa accgtacccg ccgcgttggt gaattcacca
    13621 atctggaacc aggcgatggt gttcagcacc tgatgcagac cggttgggat cagcagacgg
    13681 ttgatgaaac caaagatacc ggaacccagc gcgcccgcag aaacgatcca ctcgcctcct
    13741 gcatggatag cgtgctgtac cggcggccag acgtaaccaa aaatggccgc cagcaccagg
    13801 cagaagaagc cggtggcaat cggcacaaag cgtttgccgc cgaagaagct caggaagtcc
    13861 ggcagtttaa tatcggacca acggttatag gctgcgccac caaccagacc ggtaatgata
    13921 cccgccagta cacccatgtt tatttctggg ttgatggtca ccatcgcttt ggttaacaca
    13981 aagtaaccta ccgcacccgc cagcgccgcc gcacctgcgc tgtctttcga ccagctggat
    14041 gccacaccga tggcgaagat taatgcgagg ttatcaaaaa tcgcaccgcc cgcctgggca
    14101 ataaacgcaa cgttaagtaa atctggctga ccgaatcgca gcaacagtgc cgccaccggc
    14161 agcaccgcga tagggagctg taacgcccta ccgagtcgct ggaaaaaacc taaaatattc
    14221 atcttattcc ccctacgaga accctatttg gctcgtttaa agccgtattt ttattttgct
    14281 gcaaattgta ctgccgatgt tctgtaatca gattgttaga tcatctgcta cagagtgtgt
    14341 gaaaatttaa ttcgtatcgc aaattaaacg cgtgtctttt gtgagttttg tcaccaaata
    14401 tcgttatcat cactcccttt tactggctaa accagaaaac ttattttatc attcaaaaaa
    14461 tcaggtcgga ttgacgcctg tctgcgcaaa tccaagttac gcttaaagat gcctaatccg
    14521 ccaacggctt acattttact tattgaggtg aataatgaga ctgatccccc tgactaccgc
    14581 tgaacaggtc ggcaaatggg ctgctcgcca tatcgtcaat cgtatcaatg cgttcaaacc
    14641 gactgccgat cgtccgtttg ttctgggcct gccaactggc ggcacgccga tgaccaccta
    14701 taaagcgtta gtcgaaatgc ataaagcagg ccaggtcagc tttaagcacg ttgtcacctt
    14761 caacatggac gaatatgtcg gtctgccgaa agagcatccg gaaagctact acagctttat
    14821 gcaccgtaat ttcttcgatc acgttgatat tccagcagaa aacatcaacc ttctcaacgg
    14881 caacgccccg gatatcgacg ccgaatgccg ccagtatgaa gaaaaaatcc gttcttacgg
    14941 aaaaattcat ctgtttatgg gcggtgtagg taacgacggc catattgcat ttaacgaacc
    15001 ggcgtcttct ctggcttctc gtactcgtat caaaaccctg actcatgaca ctcgcgtcgc
    15061 gaactctcgt ttctttgata acgatgtcaa tcaggtgcca aaatatgccc tgactgtcgg
    15121 tgttggtaca ttgctggatg ccgaagaagt gatgattctg gtgctgggta gccagaaagc
    15181 actggcactg caggctgcag ttgaaggttg cgtgaaccac atgtggacca tcagctgtct
    15241 gcaactgcat ccaaaagcga tcatggtgtg cgatgaacct tccaccatgg agctgaaagt
    15301 taagacttta agatatttca atgaattaga agcagaaaat atcaaaggtc tgtaattgtt
    15361 atccctgccc tggctccttg ctcagggcaa tattttttaa aatcgggggt cagaatgtat
    15421 gcattaaccc agggccggat ctttaccggc cacgaatttc ttgatgacca cgcggttgtt
    15481 atcgctgatg gcctgattaa aagcgtctgt ccggtagcgg aactgccgcc agagatcgaa
    15541 caacgttcac tgaacggggc cattctctcc cccggtttta tcgatgtgca gttaaacggc
    15601 tgcggcggcg tacagtttaa cgacaccgct gaagcggtca gcgtggaaac gctggaaatc
    15661 atgcagaaag ccaatgagaa atcaggctgt actaactatc tgccgacgct tatcaccacc
    15721 agcgatgagc tgatgaaaca gggcgtgcgc gttatgcgcg agtacctggc aagacatccg
    15781 aatcaggcgt taggtctgca tctggaaggt ccgtggctga atctggtaaa aaaaggcacc
    15841 cataatccga attttgtgcg taagcctgat gccgcgctgg tcgatttcct gtgtgaaaac
    15901 gccgacgtca ttaccaaagt gaccctggca ccggaaatgg ttcctgcgga agtcatcagc
    15961 aaactggcaa atgccgggat tgtggtttct gccggtcact ccaacgcgac gttgaaagaa
    16021 gcgaaagccg gtttccgcgc ggggattacc tttgccaccc atctgtacaa cgcgatgccg
    16081 tatattaccg gtcgtgaacc gggcctggcg ggcgcgatcc tcgacgaagc tgacatttat
    16141 tgcggtatta tcgctgatgg cctgcatgtt gattacgcca acattcgtaa cgctaaacgc
    16201 cttaaaggcg acaaactgtg tctggttacc gacgccaccg cgccagcagg tgccaacatt
    16261 gaacagttca tttttgcggg taaaacaata tactaccgta acggactttg tgtggatgag
    16321 aacggtacgt taagcggttc atccttaacc atgattgaag gcgtgcgtaa tctggtcgaa
    16381 cattgcggta tcgcactgga tgaagtgctg cgtatggcga cgctctatcc ggcgcgtgcg
    16441 attggcgttg agaaacgtct cggcacactc gccgcaggta aagtagccaa cctgaccgca
    16501 ttcacacctg attttaaaat caccaagacc atcgttaacg gtaacgaggt cgtaactcaa
    16561 taagagaaag tatgacacca ggcggacaag ctcagatagg taatgttgat ctcgtaaaac
    16621 agcttaacag cgcggcggtt tatcgcctga ttgaccagta cgggccaatc tcgcggattc
    16681 agattgccga gcaaagccag cttgcccccg ccagcgtaac caaaattacg cgtcagctta
    16741 tcgaacgcgg gctgatcaaa gaagttgatc agcaggcctc caccgggggc cgccgcgcta
    16801 tctccatcgt caccgaaacc cgcaatttcc acgcaatcgg cgtacggctt ggtcgtcacg
    16861 acgccaccat caccctgttt gatctcagca gcaaagtgct ggcagaagaa cattacccgc
    16921 tgccggaacg tacccagcaa acgctggaac atgccctgtt gaatgccatt gctcagttta
    16981 ttgatagcta ccagcgcaaa ctgcgcgagc tgatcgcgat ttcggtgatc ctgccagggc
    17041 ttgttgaccc ggacagcggc aaaattcatt acatgccgca tattcaggta gaaaactggg
    17101 ggctggtaga agctctggaa gaacgtttta aagtgacctg tttcgttggt cacgatatcc
    17161 gtagtctggc gctggcggag cactacttcg gtgcaagtca ggattgcgaa gactccattc
    17221 tggtgcgtgt ccatcgcgga accggggccg ggattatctc taacgggcgc atttttattg
    17281 gccgcaacgg taacgtcggt gaaattggcc atattcaggt cgaaccgctg ggtgaacgct
    17341 gccactgcgg caactttggc tgcctggaaa ctatcgccgc caacgctgcc attgaacaac
    17401 gggtgttgaa tctgttaaag cagggctacc agagccgcgt gccgctggac gactgcacca
    17461 tcaaaactat ctgcaaagcc gcgaacaaag gcgatagtct ggcgtcggaa gtaattgagt
    17521 atatcggtcg tcatctgggt aaaaccatcg ccattgctat caacttattt aatccgcaaa
    17581 aaattgttat tgccggtgaa atcaccgaag ccgataaagt gctgctccct gctattgaaa
    17641 gctgcattaa tacccaggcg ctgaaggcgt ttcgcactaa tctgccggtg gtacgttctg
    17701 agctggatca ccgctcggca atcggcgctt ttgcgctggt aaaacgcgcc atgctcaacg
    17761 gtattttgct ccagcatttg ctggaaaatt aatgtgcttt tatagtggcg cttattgttg
    17821 tcaatattct gggtagtcca tgaccattaa aaatgtaatt tgcgatatcg acggcgtgct
    17881 gatgcacgat aacgtcgccg taccgggtgc agcggaattt ttgcacggga ttatggataa
    17941 aggcctgccg ctggtgttgc tgaccaacta tccttcgcag actgggcaag atctggcgaa
    18001 ccgctttgcc accgcaggtg tcgatgtacc tgacagcgtg ttttatacct ctgcgatggc
    18061 gactgccgat ttcctgcgtc gccaggaagg caagaaagcg tatgtggtgg gcgaaggcgc
    18121 actgattcat gaactgtaca aagccggttt cactattacc gatgtgaacc ctgatttcgt
    18181 gattgttggc gaaacgagtt cctacaactg ggacatgatg cataaagcag cctatttcgt
    18241 cgctaacggt gcacgtttta tcgccaccaa tccggacacc cacgggcgcg gtttttatcc
    18301 cgcgtgtggc gcgttgtgtg caggcatcga aaaaatctcc gggcgtaaac cgttctatgt
    18361 tggtaaaccg agtccgtgga tcatccgcgc ggcattaaac aaaatgcagg cgcattcgga
    18421 agaaacggtg attgtcggcg ataacctgcg taccgatatt ctggcgggct tccaggcagg
    18481 tctggaaacg attctggtgc tttctggtgt ttcatcgctc gacgatatcg acagtatgcc
    18541 gttccgccca agctggattt acccgtcggt cgctgaaatc gacgttatct gaatagcaac
    18601 cacgtctgcg gacgtggttg gctctgatga caaacgccaa actgcctgat gcgctacgct
    18661 tatcaggcct acgcagctcc tgcaatatat tgaattttca tgcttttgta ggccggataa
    18721 ggcgttcacg ccgcatccgg catgaacaaa ttgcactttg tcagcaattt ggcccccgta
    18781 aaatacaaac attcaataaa acccgctgaa aattatcgat tcatcaataa atatatcctc
    18841 acaccagacc ttttttcacc aatcgacaat caccattacg ttttttattt tttcaccgcc
    18901 aaatcgcttg cgcacagtgc tgatttgcgg cattttttta aacaagcaaa cacaacaagc
    18961 aacaaatacc agattaacgg agaaggttat gtgttcaatt tttggcgtat tcgatatcaa
    19021 aacagacgca gttgagctgc gtaagaaagc actcgagctg tcacgcctga tgcgtcatcg
    19081 tggcccggac tggtccggta tttatgccag cgataacgcc attctcgctc acgaacgtct
    19141 gtcaattgtt gacgttaacg cgggggcgca acctctctac aaccaacaaa aaacccacgt
    19201 actggcggta aacggtgaaa tctacaacca ccaggcattg cgcgctgaat atggcgatcg
    19261 ttatcagttc cagacagggt ctgactgtga agtgatcctc gcgctgtatc aggaaaaagg
    19321 gccggaattt ctcgacgact tgcagggcat gtttgccttt gccctgtacg acagcgaaaa
    19381 agatgcctac ctgattggtc gcgaccatct ggggatcatc ccactgtata tgggctatga
    19441 cgaacacggt cagctgtatg tggcctcaga aatgaaatcg ctggtgccag tttgccgcac
    19501 gattaaagag ttcccggcgg ggagctattt gtggagccag gacggcgaaa tccgttctta
    19561 ttatcatcgc gactggttcg actttgatgc agtgaaagat aacgtgaccg acaaaaacga
    19621 gctgcgtcag gctctggaag attccgttaa aagccatctg atgtctgatg tgccttacgg
    19681 tgtgctgctt tctggtggtc tggattcctc aattatttcc gctatcacca agaaatacgc
    19741 agcccgtcgc gtggaagatc aggaacgctc tgaagcctgg tggccgcagt tacactcctt
    19801 tgctgtaggt ctgccgggtt caccggatct taaagcagcc caggaagtgg caaaccatct
    19861 gggcacggtg catcacgaaa ttcacttcac tgtacaggaa ggtctggatg ccatccgcga
    19921 cgtgatttac cacatcgaaa cttatgatgt gaccactatt cgcgcttcaa caccgatgta
    19981 tttaatgtcg cgtaagatca aggcgatggg cattaaaatg gtgctgtccg gtgaaggttc
    20041 tgatgaagtg tttggcggtt atctttactt ccataaagcg cccaacgcta aagaactgca
    20101 tgaagagacg gtgcgtaaac tgctggccct gcatatgtat gactgcgcgc gcgccaacaa
    20161 agcgatgtca gcctggggcg tggaagcacg cgttccgttc ctcgacaaaa aattccttga
    20221 tgtggcgatg cgcattaacc cgcaggataa aatgtgcggt aacggcaaaa tggaaaaaca
    20281 catcctgcgt gaatgttttg agtcatacct gcccgcaagc gtggcctggc ggcagaaaga
    20341 gcagttctcc gatggcgtcg gttacagttg gatcgacacc ctgaaagaag tggcggcgca
    20401 gcaggtttct aatcagcaac tggaaactgc ccgcttccgc ttcccgtaca acacgccaac
    20461 ctcaaaagaa gcgtatctgt accgggagat ctttgaagaa ctgttcccgc ttccgagcgc
    20521 cgctgagtgc gtgcctggcg gtccttccgt cgcgtgttct tccgctaaag cgatcgaatg
    20581 ggatgaagcg ttcaagaaaa tggacgatcc atctggtcgt gcggttggtg ttcaccagtc
    20641 ggcatataag taagaaatgc gaaacagccc cggtgaaatc cggggctgtt tcagttattg
    20701 aggtgcttgt tgattctgat atgcagtaag gaaatgttcc gggtttgcca catcactgtt
    20761 tgacaactca tcactgtagg ccagtatttc atagattaat ttcccaccac tattgttgaa
    20821 ttccgaattt catcctgtac gacaaaagaa tggctgtagg gtggggaaaa aacacaaagt
    20881 aaacaataat tgacgaatat agcgccacgc tgttcgcaac ctaaccaaat agtcactttc
    20941 gagcaatttt ccttgaaaaa gaggttgacg ctgcaaggct ctatacgcat aatgcgcccc
    21001 gcaacgccga taaggtatcg cgaaaaaaaa gatggctacg tagctcagtt ggttagagca
    21061 catcactcat aatgatgggg tcacaggttc gaatcccgtc gtagccacca tctttttggc
    21121 gggagtggcg aaattggtag acgcaccaga tttaggttct ggcgccgcaa ggtgtgcgag
    21181 ttcaagtctc gcctcccgca ccattcacca gaaagtgttg tacggatggg gtatcgccaa
    21241 gcggtaaggc accggttttt gataccggca ttccctggtt cgaatccagg taccccagcc
    21301 atcttcttcg agtaagcggt tcaccgcccg gttattgggg tatcgccaag cggtaaggca
    21361 ccggtttttg ataccggcat tccctggttc gaatccaggt accccagcca tcgaagaaac
    21421 aatctggcta cgtagctcag ttggttagag cacatcactc ataatgatgg ggtcacaggt
    21481 tcgaatcccg tcgtagccac caaattctga atgtgtcgaa tacgttcggc aaattcaaaa
    21541 accaatttgt tggggtatcg ccaagcggta aggcaccgga ttctgattcc ggcattccga
    21601 ggttcgaacc ctcgtacccc agccaattta ttcaagacgc ttacatcgta agtgcaccca
    21661 gttggggtat cgccaagcgg taaggcaccg gattctgatt ccggcattcc gaggttcgaa
    21721 tcctcgtacc ccagccacat taaaaaagct cgcttcggcg agctttttgc ttttctgcgt
    21781 tcattcaatg tcgaatgcga tgttgacacg tcttatcctt caatgtcgga tgcgacgctg
    21841 ccgcgtctta tccgacctac ggttggcacg catccggcaa tgttgtaagg ctacaaccct
    21901 aacgcatatt tcagcgcctg acgtttcaac acgccagcac gctctgccgc cattaacccg
    21961 agattacgca caaaacgcag cggcggcaga ttattactga atccggcata aaacagatcc
    22021 ataccacttt gcataatgaa gttatccgcc atgcgccgca tctggtaacg cttgaggaca
    22081 ggataactgg cccacgcttc accgtaactg cgggcattca ccagaacatc aatcagggca
    22141 tcgacatcac gataaccaag attcaccccc tgccccgcca gcggatggat ggtatgcgcg
    22201 gcatcgccca ccagcgcaag ccccggctgc acatactgca acgcatgtcg gcgcgtcagc
    22261 ggaaacgcac cagcggcaag cggtgtaacg taacccagac gcgacgggaa atgcttcgcg
    22321 atttccgcct ggagttgcgc catattcata ttctgcaact ggcgaatacg cgccggagag
    22381 tcataccaca ccagcgatgc ccagttatca aacaacggca gaaacgcacg cggtccgtcc
    22441 ggggtaaatt gctgccaggt gctgtcgccg ggatcgttct cacactgtac gctaatcaac
    22501 atgcacgact gcgcatactg ccaggcatga acgccaattc ccgccatctg ccgcacctga
    22561 gaatttgcgc cgtcggcacc aatcaccagt ttcgcgcgga tcgtttcacc gcccttcagt
    22621 tccagctcct gaagatcatt atctcgatgt aatgcaatca gcgagcctgg cacacgtaac
    22681 gttactttcg gatgcgcttc cagcgcctgc cacagcgcct gttgcaggac ggtgttttcc
    22741 accatatagc caagcaacgg tagcttaagt tcagcggcgt caaacaccac atgcgccgtt
    22801 tcccactccc acgtttccag tctgcggtaa ggatggcaac gcatagcctg tactgcatcc
    22861 cagaccccta accctttaag caatgatacc gatgcggcgc tgatcgccga gatccgcacg
    22921 tccggttggc tatcagcgac aaacggtgca ggttctgcgt gctcgatcac cgttaccgaa
    22981 aatccgtgct gtgccagccc cagcgccagc gcgccgccga ccattcctcc gccgacaatg
    23041 gcaatttccg ttggttgatt tgtcatggtc aatcatcctg ttagcaatat gctttaagtt
    23101 tacaggattt ttacccccaa gggctgataa cgctcatact ggccacaacg gcagcaaagc
    23161 attacactat gcgccctgca ttcctggcta ctatttcgca agagcaagtc gatgaccaaa
    23221 aaactccata ttaaaacctg gggctgtcag atgaacgagt acgattcatc gaagatggcc
    23281 gatctgctgg atgccaccca cggctatcaa ctgaccgacg tggcggaaga agcggatgtg
    23341 ctgctgctga acacctgctc aatccgcgag aaggctcagg aaaaagtctt ccatcagttg
    23401 ggtcgctgga aactgttaaa agagaagaat ccagacctga ttatcggcgt cggtggctgc
    23461 gtggcatcgc aagaaggcga gcacattcgc cagcgcgccc actatgtcga tattattttt
    23521 gggccgcaaa cgctgcaccg tctgccggag atgatcaact ccgtgcgcgg cgaccgcagc
    23581 ccggttgtag atatcagctt cccggaaatc gagaagtttg accgtctacc ggaaccgcgc
    23641 gccgaagggc cgaccgcgtt tgtctccatc atggaaggct gcaataaata ttgcacctac
    23701 tgcgtggtgc cttacacccg tggtgaagag gtaagccgtc cgtctgacga tattctgttt
    23761 gagattgccc agcttgcggc tcagggcgtg cgtgaagtca acctgctcgg tcagaacgtg
    23821 aacgcctggc gtggtgaaaa ctacgacggc tccaccggat cgtttgccga tctgctgcgt
    23881 ctggttgctg cgatcgacgg gatcgatcgt attcgcttta ccaccagcca tccgatcgaa
    23941 ttcaccgacg atatcatcga agtgtatcgc gacacgccgg agctggtgag cttcctgcat
    24001 ctgccggtac agagcggttc cgatcgcatt ctgaacctga tgggccgtac tcatacggca
    24061 ctggagtaca aagcgatcat ccgtaaactg cgtgcggcgc gtccagatat tcagatcagt
    24121 tctgatttca tcgttggctt ccctagcgaa accaccgaag acttcgagaa aacgatgaag
    24181 ctgattgccg acgtcaattt cgacatgagc tacagcttta tcttctctgc acgtccgggt
    24241 acaccagccg ccgatatggt tgatgatgtt ccggaagaag agaagaagca gcgtctgtat
    24301 attctgcaag aacgtatcaa tcagcaagcg atggcatgga gccgccggat gctcggcacc
    24361 acccagcgta ttctggtaga agggacttcg cgtaagagca tcatggagct ttccgggcgt
    24421 accgaaaata accgcgtggt caacttcgag ggcacaccgg atatgatcgg taaattcgtc
    24481 gatgtagaaa ttaccgacgt ctacccgaac tctctgcgcg gtaaagtggt gcgtactgaa
    24541 gatgaaatgg gcctgcgcgt ggcagaaaca ccggaatcgg tgattgcccg tacccgcaaa
    24601 gaaaacgacc ttggcgtggg ttattatcag ccgtaattct caggcccgcc gttccggtgg
    24661 gccttgtttt ttctctcttt atccccatct ttatcgcaat gcttgcgcct ttttcctgtg
    24721 gcgtgaataa tttccttata ggccggtgaa tgttcagctt ccggcggcat acagataaga
    24781 ggaacggttt gaacatagac actcgcgaaa tcaccctgga gccagcagac aacgcgcgtc
    24841 tgttgagcct gtgcggcccg tttgatgaca acatcaagca gctcgaacgc cgtctcggca
    24901 tcgagatcaa tcgccgcgac aaccacttta aactgaccgg ccgtccgatt tgcgtcaccg
    24961 ctgcggcaga cattctgcgt agcctgtatg tcgatacagc cccgatgcgc ggtcagattc
    25021 aggatatcga accggaacag atccacctta cgattaaaga agcgcgagta ctggagcaaa
    25081 gtgcggagag cgtgccggag tacggcaaag cggtcaatat caaaaccaaa cgcggcgtaa
    25141 ttaagccgcg cacgccaaac caggcgcagt acatcgccaa tattctcgac catgacatta
    25201 ccttcggcgt tggcccggcg ggtacgggta aaacctacct ggcagtggct gcggcagttg
    25261 atgccctgga gcgtcaggag attcgccgta ttctgctgac tcgtccggcg gtcgaagccg
    25321 gtgagaaact gggcttcctg cctggcgatt taagccagaa agtagacccg tatctgcgcc
    25381 cactgtacga cgcgctgttt gaaatgctgg gctttgagaa agttgagaaa ctgattgagc
    25441 gcaacgttat tgaagtcgcg ccgctggcct atatgcgtgg tcgtacgctg aacgacgcgt
    25501 ttatcattct cgatgagagc cagaacacca ccatcgaaca gatgaagatg ttcctgaccc
    25561 gtatcggttt taactcaaaa gcggttatca ccggcgacgt cacacagatc gacctgccgc
    25621 gtaatactaa atcaggctta cgtcacgcca tcgaagtact ggccgatgtc gaagagatca
    25681 gctttaactt cttccacagc gaagacgtgg ttcgtcaccc tgtggtggcg cgtatcgtta
    25741 acgcctatga agcctgggaa gaagccgaac aaaaacgaaa agcggcgctg gcggcagaac
    25801 gcaagcgcga agaacaggaa caaaaatgag tcaggtgatc ctcgatttac aactggcatg
    25861 tgaagataat tccgggttac cggaagagag ccagtttcaa acatggctga atgcggtgat
    25921 cccgcagttt caggaagaat cggaagtgac tattcgcgtg gtcgataccg ccgaaagcca
    25981 cagtctgaat ctgacctatc gcggtaagga taagccgacc aacgtgctct ccttcccgtt
    26041 tgaagtgccg cctggcatgg aaatgtcgct actgggcgat ctggttatct gccgtcaggt
    26101 ggttgagaag gaagctcagg agcaaggcaa accactggag gcgcactggg cgcatatggt
    26161 ggtgcacggc agtctgcatt tgttaggtta cgatcacatc gaagatgacg aagcagaaga
    26221 aatggaagcc ctcgaaacag agattatgct tgctctgggc tatgaggatc cgtacattgc
    26281 cgagaaagaa taatttgcca ggcatttgac tggcagacgt ccccgccgtt ggtgattaac
    26341 gacggcgttt tgattaacta acatgagaac ccatacgacg ccatgagcga cgacaattca
    26401 cacagtagtg acactataag caacaagaag ggatttttct ccctgttact cagccaactt
    26461 ttccacggtg aaccgaaaaa ccgtgacgaa ctgctggcgc tgatccgtga ttccgggcag
    26521 aacgacctta tcgacgaaga tacgcgcgat atgctcgaag gggtgatgga catcgcagac
    26581 caacgcgttc gcgacatcat gatcccccgc tcccagatga ttaccctgaa acgcaaccag
    26641 acgctggacg aatgccttga tgtcatcatc gagtccgccc actcacgttt cccggtgatt
    26701 agcgaagaca aagatcacat tgaagggatt ctgatggcga aagacttgct gccgtttatg
    26761 cgcagcgatg ctgaagcctt cagcatggac aaagtgttac gtcaggcggt tgtcgttcct
    26821 gaaagtaagc gcgtagaccg gatgctgaaa gagtttcgct ctcagcgtta ccacatggcg
    26881 atcgtcattg acgaattcgg tggggtttcc ggtctggtga ccattgaaga catcctggaa
    26941 ctgattgttg gtgagattga agacgaatat gacgaagaag atgatatcga cttccgtcag
    27001 ctgagtcgtc atacctggac cgtgcgcgca ctggcttcca ttgaagactt caacgaagcg
    27061 ttcggcaccc actttagcga tgaagaagtc gacactatcg gtggtctggt gatgcaggcg
    27121 tttgggcatc ttccggcgcg tggcgaaact atcgacatcg acggttacca gttcaaagtg
    27181 gcgatggccg acagtcgacg tattattcag gttcatgtca aaatcccgga tgactcaccc
    27241 cagccgaagc tggatgaata aaaccgaaac tggatagata actacatggc ttttgcctca
    27301 ttaattgaac gccagcgcat tcgcctgctg ctggcgttat tattcggtgc ctgcggaacg
    27361 ctggccttct ctccttacga cgtctggcct gcggcgatta tttcgttgat ggggcttcag
    27421 gcgttgacct ttaaccgccg tccactccag tctgccgcta ttggcttttg ctggggattt
    27481 ggcctctttg gcagcggtat taactgggtc tatgtcagca tcgcgacctt tggcggaatg
    27541 cctggcccgg ttaacatctt cctggtggtg ctgctggcgg cgtatttgtc gctgtatacc
    27601 ggactgtttg ctggcgtgct gtcgcgtctg tggccgaaaa ccacctggct gcgcgtggcg
    27661 attgccgccc ctgctctctg gcaagtgacc gagtttctgc gcggttgggt actgacaggc
    27721 ttcccgtggt tacagttcgg ctatagccag attgatggcc cgttaaaagg gctggcaccg
    27781 ataatgggcg tggaagccat taacttcctg ctgatgatgg ttagtggcct gctggcactg
    27841 gcgttggtca aacgcaactg gcgtccgctg gtggtggccg tcgtgctgtt tgccctaccc
    27901 ttcccgctgc gttacatcca atggtttacc ccgcaaccgg agaaaaccat tcaggtttcg
    27961 atggttcagg gcgatattcc gcaatcactg aaatgggatg gagatcagct tcttaatacg
    28021 ctgaagattt actacaacgc aacggctccg ctgatgggca aatcatcgtt gattatctgg
    28081 ccggagtcgg cgataaccga tctggaaatt aatcagcaac cgttcctcaa agcactggac
    28141 ggtgagttgc gcgataaagg tagctcgctg gtaaccggga ttgtcgacgc gcgtctcaat
    28201 aagcagaacc gctacgatac ctacaacacc atcatcacac tgggtaaagg tgcaccgtac
    28261 agctacgaat cagccgatcg ctataacaaa aaccatctgg tgccgtttgg cgagtttgtc
    28321 ccgctggagt cgattctgcg tccgttagca ccgttctttg atctgccgat gtcgtcgttc
    28381 agccgtgggc catatatcca gccgccgctg tcagtaaatg gtattgagct tactgcggct
    28441 atttgctacg agatcattct cggcgagcaa gtgcgcgata acttccgccc ggataccgac
    28501 tatctgctga ctatctccaa cgatgcgtgg tttggtaaat ctattggtcc atggcaacac
    28561 ttccagatgg cgcgaatgcg tgcgctggag ctggcgcgcc cactgttgcg cagcaccaac
    28621 aacggcatta cggcggtgat tggcccgcag ggtgagattc aggcgatgat cccgcagttc
    28681 acccacgagg tgttaaccac taatgtgaca ccgaccaccg gactcacacc gtatgcacgt
    28741 accggcaact ggccactgtg ggtgctgacg gcactgtttg gttttgccgc tgtgttgatg
    28801 agtctgcgag cacgtaaacg ttgatgtgcc tgatgtatcc tgcatgagca aaacgtactt
    28861 tctcattgag ctttggggca tggttatcat gccccttttt cttacggttc agggatacaa
    28921 gccacattgc catattgggg atgacgaata attgcctgcg gatcaagcga gcgcgtcctg
    28981 tccatttgga tcaacccgtt cttcgccatg acaaatattt ttctaccatc cgctgaa
//
LOCUS       DAJTMC010000034        28531 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.034, whole genome shotgun sequence.
ACCESSION   DAJTMC010000034 DAJTMC010000000
VERSION     DAJTMC010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28531
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.034"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(<1..94)
                     /locus_tag="OO130_001343"
     CDS             complement(<1..94)
                     /locus_tag="OO130_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCR5364817.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIITAAIVSWW"
     gene            complement(499..1599)
                     /gene="chaA"
                     /locus_tag="OO130_001344"
     CDS             complement(499..1599)
                     /gene="chaA"
                     /locus_tag="OO130_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HCR5364818.1"
                     /translation="MSNAQEAVKTRHKEISLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSVWHAIWLIIHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            1869..2099
                     /gene="chaB"
                     /locus_tag="OO130_001345"
     CDS             1869..2099
                     /gene="chaB"
                     /locus_tag="OO130_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HCR5364819.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKDDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            2257..2952
                     /gene="chaC"
                     /locus_tag="OO130_001346"
     CDS             2257..2952
                     /gene="chaC"
                     /locus_tag="OO130_001346"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HCR5364820.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGCTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(2996..3349)
                     /gene="ychN"
                     /locus_tag="OO130_001347"
     CDS             complement(2996..3349)
                     /gene="ychN"
                     /locus_tag="OO130_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HCR5364821.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            3533..4928
                     /gene="ychO"
                     /locus_tag="OO130_001348"
                     /pseudo
     CDS             3533..4928
                     /gene="ychO"
                     /locus_tag="OO130_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
     gene            complement(4929..5579)
                     /gene="narL"
                     /locus_tag="OO130_001349"
     CDS             complement(4929..5579)
                     /gene="narL"
                     /locus_tag="OO130_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HCR5364822.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(5572..7368)
                     /gene="narX"
                     /locus_tag="OO130_001350"
     CDS             complement(5572..7368)
                     /gene="narX"
                     /locus_tag="OO130_001350"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HCR5364823.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDEQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRVRLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPASSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            7707..9098
                     /gene="narK"
                     /locus_tag="OO130_001351"
     CDS             7707..9098
                     /gene="narK"
                     /locus_tag="OO130_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HCR5364824.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            9614..13336
                     /locus_tag="OO130_001352"
     CDS             9614..13336
                     /locus_tag="OO130_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HCR5364825.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERAYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNQLMLTLGRGGPVVWLSEADAKELGIADNDWIEVYNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITGQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGND"
     gene            13354..14892
                     /gene="narH"
                     /locus_tag="OO130_001353"
     CDS             13354..14892
                     /gene="narH"
                     /locus_tag="OO130_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HCR5364826.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLEVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            14889..15599
                     /gene="narJ"
                     /locus_tag="OO130_001354"
     CDS             14889..15599
                     /gene="narJ"
                     /locus_tag="OO130_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HCR5364827.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEVIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            15599..16276
                     /gene="narI"
                     /locus_tag="OO130_001355"
     CDS             15599..16276
                     /gene="narI"
                     /locus_tag="OO130_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HCR5364828.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFAGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(16459..16590)
                     /locus_tag="OO130_001356"
     ncRNA           complement(16459..16590)
                     /ncRNA_class="other"
                     /locus_tag="OO130_001356"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(16638..16722)
                     /locus_tag="OO130_001357"
     tRNA            complement(16638..16722)
                     /locus_tag="OO130_001357"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16686..16688),aa:Tyr,seq:gta)
     gene            complement(16757..16841)
                     /locus_tag="OO130_001358"
     tRNA            complement(16757..16841)
                     /locus_tag="OO130_001358"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16805..16807),aa:Tyr,seq:gta)
     gene            complement(17001..17843)
                     /gene="purU"
                     /locus_tag="OO130_001359"
     CDS             complement(17001..17843)
                     /gene="purU"
                     /locus_tag="OO130_001359"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HCR5364829.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVNHEGLSRNEHDQKMADAIDAYK
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            complement(17893..18351)
                     /locus_tag="OO130_001360"
     CDS             complement(17893..18351)
                     /locus_tag="OO130_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HCR5364830.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMAGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            18464..19369
                     /gene="rssA"
                     /locus_tag="OO130_001361"
     CDS             18464..19369
                     /gene="rssA"
                     /locus_tag="OO130_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HCR5364831.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQCREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRVLGADIVIAVDLQHDAHLIQQDLLSFNVSEENSENGESLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            19461..20474
                     /gene="rssB"
                     /locus_tag="OO130_001362"
     CDS             19461..20474
                     /gene="rssB"
                     /locus_tag="OO130_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HCR5364832.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDATVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            20676..21584
                     /gene="galU"
                     /locus_tag="OO130_001363"
     CDS             20676..21584
                     /gene="galU"
                     /locus_tag="OO130_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HCR5364833.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEQVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(21728..22141)
                     /gene="hns"
                     /locus_tag="OO130_001364"
     CDS             complement(21728..22141)
                     /gene="hns"
                     /locus_tag="OO130_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HCR5364834.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            22746..23363
                     /gene="tdk"
                     /locus_tag="OO130_001365"
     CDS             22746..23363
                     /gene="tdk"
                     /locus_tag="OO130_001365"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HCR5364835.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            complement(23645..24541)
                     /locus_tag="OO130_001366"
                     /pseudo
     CDS             complement(23645..24541)
                     /locus_tag="OO130_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904641.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            complement(24665..27340)
                     /gene="adhE"
                     /locus_tag="OO130_001367"
                     /gene_synonym="adhC"
     CDS             complement(24665..27340)
                     /gene="adhE"
                     /locus_tag="OO130_001367"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HCR5364836.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGILSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
     gene            27818..28465
                     /gene="ychE"
                     /locus_tag="OO130_001368"
     CDS             27818..28465
                     /gene="ychE"
                     /locus_tag="OO130_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HCR5364837.1"
                     /translation="MIQTFFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
BASE COUNT         6996 a         6865 c         7437 g         7233 t
ORIGIN      
        1 gccaccagct gacaatcgct gcggtaataa tccccgccag gatcggtgct gccaggtcgt
       61 gccagaaagt catggcaaac tgcgcgagcg tcatatagcc gccttgttgt aatgacaaca
      121 ttttgcggct attcttgaag tgtctggttt caagattagc ccccgttttg ttgtcaggtt
      181 ttacctctca acgtgcgggg gttttctctt tccagcaacc aatgccacca gggataaagc
      241 ccccgcaaca ttgcgcctca ccggataatt ccggcttggt gtggatacta cgtctcaatt
      301 catcttcact tcatccctga aatgtttgca ataaagagta cattccggct tttcaacagc
      361 tgttgcagtg gtttcgtgca ggttgtggta caggctcgca attctgatta acgagctgtc
      421 caggtagtat gaagaaatac aataagcagg aatttatctt atctcccccg atttccgtca
      481 tcggaggaga tataactgtc aggcaaatat cgtcatcaaa taggcggcaa acagtgccag
      541 atgcgctgcg ccattgagca cgttagtacg tccggtggag aaggagatat ggcacagcac
      601 taaagaggcc accatcacca ccatttctgg cgcaccaagt gcaaactgta attcgttacc
      661 cgtcataaag gcaattagcg tgacgacagg tacggtaagt gaaatggttg ctaacactga
      721 accaaagaac agattcatcg cgcgctggac ctggttgttc aacactgctt ttaacgcacc
      781 taaaccttcc ggcgacagaa tcaacagagc caccaggaag ccagtaaagg cgacaggggc
      841 attcatgctg tcgagcaatg tctccagcgg gctggcattc attttggtga ctgcaataac
      901 ggcaatcaga tggataatca accagatagc atgccacacg ctgctatggg cagacggttt
      961 accgtgatgc gggtcgtcgt catcactgtc atcttcgtgc tcgtagacaa acaaactttg
     1021 atgcgttttg gtctggatca gcaaaaatac gccatacatt gccgcagaaa ttaatgctac
     1081 cagtaacgcc tgaccggttg aaaaattcgc cgcaggcaga gccattggaa ataccagtac
     1141 gattatcgcc agggggaaca gcgcaattaa atactgcttg ataccaaaca gattcatata
     1201 ttgggtggca aacttacgac cgcccaacaa taatgaaaag ccaaccagcc caccggtaac
     1261 aatcataatg attgaataga gcgtatcacg cattagcgtt ggcgcggcgt cgccggttgc
     1321 cattaaagct gaaatcaaac tgacttcaag aataaccact gaaaggctaa gaataagcga
     1381 accgtaaggt tctcccaggc gatgggctaa tacgtccgca tgacggacaa cactaaaggc
     1441 gctacttaaa ataccaataa gcgcaagaag attgatggca atgaccactg gtagtgtctg
     1501 gctgcttccc cacaggaaca gcactaccag cgccagaacc gggaaaataa gcgaaatctc
     1561 cttgtggcgg gtttttaccg cctcttgagc atttgacatt atggttatcc ctttgcagat
     1621 gaatttatcg aaaatgtaaa aaataggtag gaaaaataac agaaagtgtc tggatatcgg
     1681 taacatttta cgaattttta ccccactgtc gattttttac tcattgggac ataaaaataa
     1741 gtattacgtt tagacaatgt ttgttttagc gtttcttaag aacagtctga catgaaaatt
     1801 cttatgtttt ggcaagagta gatattgttg accacactta atgttcaact ttgtaaaagg
     1861 agtcaacgat gccgtataaa acgaaaagcg atctgccgga aagcgtaaag cacgttctac
     1921 cgtctcatgc ccaggatatc tataaagaag cgttcaacag cgcatgggat caatataaag
     1981 ataaagacga tcggcgtgat gacgccagtc gcgaagaaac cgcgcataaa gtggcctggg
     2041 ctgctgtgaa gcatgaatat gccaaagggg atgatgataa atggcataaa aaatcgtaaa
     2101 accggtagct tagttaaagc tattcgtgcg gtgttgcctt gcaagtggtc cgtggattgc
     2161 atattgtccc gttagtggtt tcaaaatgag cagtaaaaat gtccggaaga caccaaaaag
     2221 ttgtcgcagg gaagtatgca gtggcggagg tgtaaggtga taacgcgtga tttcttgatg
     2281 aatgccgatt gtaaaacggc atttggtgcc attgaagaat cactcttatg gtcagcagag
     2341 caacgagcgg cttcgctggc ggcgacgctg gcttgtcgac ctgatgaggg accggtgtgg
     2401 atcttcggtt atggatcgtt gatgtggaat ccggcactgg agtttaccga gtcgtgcacc
     2461 ggtacactgg ttggatggca tcgcgcgttt tgcctgcgcc tgaccgccgg gcgcgggact
     2521 gcgcaccagc cgggacggat gcttgcactg aaagagggcg gacgcactac aggcgtcgcc
     2581 tatcgactgc cagaagagac gctggagcag gaactaaccc tgttgtggaa gcgcgagatg
     2641 attaccggct gttatctgcc aacctggtgt cagcttgatc ttgatgatgg atgcacagta
     2701 aacgccattg tgtttattat ggacccgcga catccagaat atgaatctga cactcgcgct
     2761 caggtcatcg cgccgttgat tgcggcggcg agcggtccgc tgggaaccaa cgcacaatac
     2821 ctgttttcac tggaacagga gcttatcaaa ctgggaatgc aggacgatgg gctgaatgat
     2881 ttgctggtat cggtaaaaaa actgctggcg gagaattatc cggatggtgt gttacggccg
     2941 ggattcgcct gagtaaactt cccgcatagt ggggcgtcag acgccccctc aaacattaaa
     3001 atgtgagcac tttatcggct gacagcgtcc attgcgccag ttccacaaga gtaccgattt
     3061 ccaccccatc aatcagagga agtgtactaa tcccgcgccc gtcggtacag gttttgcaca
     3121 atttcaccgg tacattctga gcggtaagga tctccagcat ttgctgaatg ttgtagcctt
     3181 cccctggttt ttgcccgcgc aacccggctg tgaccgcatc agacatgagg aacagacgca
     3241 gatccagatt gctctcctgc tctcgtaacg caatggccag ccgcaagctg ttaaacaagg
     3301 attcgctccc gtaaggtgcg ccattggcaa cgatcacgat tttttgcatt atttactcct
     3361 gtattcaggg aattagacac tcatcttcta tcttactgct tctgcagcgt ctgaccaatc
     3421 ggtcacattt ttaaggattt tcctgaaagc gcgagaaaat acgacaaaag ttgccagtaa
     3481 tcgttattct ttaaggctat ggttttcatt attaccggaa gttaccgacg ttttgagccg
     3541 tttcgttcct cgcattatcc cgttttattt actcttgctt gtagcaggcg gtacagctaa
     3601 cgcacaatct accttcgagc aaaaagcggc aaatcccttt gataataaca atgatggtct
     3661 gccggattta ggcatggctc ccgaaaatca tgatggggaa aaacactttg ctgaaattgt
     3721 gaaagatttc ggcgaaacca gtatgaatga taacgggctg gatactggcg agcaggcaaa
     3781 agctttcgca ttgggaaaag tccgcgacgc gcttagtcaa caggttaatc agcacgtaga
     3841 gtcctggcta tcaccgtggg gaaatgccag tgttgacgtc aaagtggata acgaaggaca
     3901 tttcaccggc agtcgtggaa gctggtttgt gccgttacaa gataatgatc gttatttcac
     3961 ctggagccag cttggtctta ctcagcagga taatgggttg gtgagcaatg tgggcgttgg
     4021 gcaacgctgg gcgcgcggca actggctggt gggttataac actttttatg acaacttgct
     4081 ggacgaaaat cttcagcgag cgggctttgg tgccgaagcg tggggcgaat atttgcgatt
     4141 attggcaaac ttttatcagc cgtttgctgc atggcatgaa cagacagcca cgcaggaaca
     4201 acggatggcg cgcgggtacg acctgacagc tcggatgcgc atgccgttct atcaacacct
     4261 caataccagt gtcagcctag aaacagtatt ttggtgatcg tgttgatttg tttaactctg
     4321 gtacgggtta tcacaatccc gtcgcgttga gtctgggatt aaattacacc cctgtgccat
     4381 tagtcactgt gacggcccag cataaacagg gtgaaagtgg cgagaatcaa aataacctcg
     4441 ggctgaatct taattaccgc tttggtgtac cgctcaaaaa acaactttct gcgggcgagg
     4501 ttgccgaaag tcagtcgtta cgtggtagtc gctatgacaa tccgcagcga aataatctac
     4561 cgactcttga gtaccgacag cgaaaaacgt taacggtgtt tctggcgaca ccgccgtggg
     4621 atctaaaacc tggcgaaaca gtgccgctga aattacaaat ccgcagtcgt tacggtattc
     4681 ggcaactgat ttggcagggc gatacgcaga tattaagttt gacgccgggc gcacaagcca
     4741 acagcgcgga gggctggacg ctgatcatgc ctgactggca gaacggggaa ggggcaagca
     4801 atcactggcg attgtcggtg gtggtggaat ataaccaggg gcagcgtgtc tcctccaatg
     4861 agatcacgct aacgcttgtc gaaccgttcg atgcattgtc aaacgacgaa ctgcgctggg
     4921 aaccgtaatc agaaaatgcg ctcctgatgc acccataccg ctgcttccac gcgagacttg
     4981 agcttcattt tcttcagcat gtgcttgacg tgcactttta ctgtgctttc ggtgatatcc
     5041 aggcggcggg caatcatctt gttcggcaaa ccctgggcaa tcagcttgag aatatcgcgc
     5101 tcgcgtgggg ttaactggtt aacatcgcgc tcagtagtgg cacggttagc gcgcaagctg
     5161 gcggccagaa caggcgttaa tgcttcgctt aataccattt cgccagcagc tgcctgatgc
     5221 aatgctttca gcagatcttc cggttccata tcttttaaca gataaccatc cgcgccgcgt
     5281 ttcagtgcgg tgaccacatc ttcttcatgg ttagagacgc tgaataccac aatgcgccct
     5341 gagagggact tttcgcgcag tttatccagc gtttccagac cgttcatgcc gggcatattg
     5401 agatctaaca ggatcagatc gggatcaaga gactccgcca gttcaatacc ctgttcgcca
     5461 ttactcgctt cgccaaccac ggtgatatct ggtgccatac tgataagctg ttttacgcca
     5521 gttcgcagca tcgggtgatc gtcgatcagc aggatagtag ccggttcctg attactcatg
     5581 ggtatctcct tggacgtctg tgaaagtttt ttcgggaata aaggtgacca ccacttcggt
     5641 gccacctgat tcacgacggc ggacgcggca atcgcctcgt aaactttgcg cacggtcgcg
     5701 cattattatc atgccgtagt gattgctgcg gatggcattt tcaggcacgc cgcagccgtt
     5761 atcttggacg gtcagtttga cttgattatc gttttgcgcc accgtcacca cgacctcact
     5821 cgcttgcgaa tgtttgaggg cgttacttaa tgcctcacgg gcaatttgca acaagtggat
     5881 tgcctgatgt gaaggcacca gacgaggcgg caattgataa tccagcttca ccgggaagcc
     5941 aaatttggcg ctgtactctt cgcaactcgc ctccagcgcc ggacgtaatc caggctcggt
     6001 gagctgcaag cggaatgtgg tgagcaattc acgcaactgc gcccaggatg cattcagttc
     6061 gttacggatc tgacttaaca gttcgcggct gcttgctggc agcgcatcgc cctgcatctg
     6121 taaacaactc acctgcatct tcatgcaaga gagagattgg gcaatagaat catgcagttc
     6181 gcgcgcaatg gtggcacgct cttccatcac gatcaactgt tgctgacgtt cctgatggcg
     6241 atccagcgcc agcgtggcgg tgagttgttc aaccagggta tccaccagtt gttgttgatc
     6301 atggctaaga tgacgcccct gcggcagggt cgccagcaaa ataccgtact gcgtatgaga
     6361 gtcagccagc cgccacttca gggttgtgcc gcgatcacca acgggtaata cgccgcgcgg
     6421 gcagagctgg cagcctttat catcacaagt catatctggc tggcaggtaa actcctgatg
     6481 attctcttca tcatcagtgt catacacccg cagttcgata tcacgtagca gggttaaatt
     6541 ctgtaagccg ttgagtacag gtgacaggcg ttcacacagc ggggcgcggg aatgcaaacg
     6601 gcggttagcc tgccataaaa aagagaggat ctgattttta tgctccagcc cggcggtttt
     6661 ctcctgaacc cgctgctcaa gtacggcata actttcggcc agttctgcag acatattgtt
     6721 caatgcagtt ccaagcatcg ccatttcgtt gcgcccgctg atgttcgcgc gttgggtaaa
     6781 atcgcgatga ctgacggcac tcgccattgc cagcagttgc cgccacggtt gtagcagtcg
     6841 cacccgcaac cagataatag tgaacaccag taaaagtgcc ataaataccg ccattacccg
     6901 atggaccagt accactgtct caatgcgcat ttccgtggtg cggtcaaaac cagataccag
     6961 ctgatcaagc ccggcaacaa actggctgac atccgctgac accgtttcgc ggttttgtgc
     7021 acgcatcagt gcagggatca gttcattacg ccagtaatct tgtaaaccct gtaattgcgc
     7081 cagttgttcg tctcgttctg ctgctcgagt taactcggcg ctaaatgccg tttgttccat
     7141 ctctttaatt aagggcttgt ctttctcact taatggcact gccgccaaca gacggtaact
     7201 ttgcatgcgc agcgatcccg ctttgttgat cgcatgggcg ctgccctgaa cgccttgcac
     7261 cagccagcca gaaaccgcca tccctgccag tccaatagca gtagaaagca acacaataag
     7321 cgcaacctga ttaaccaggg tgagcggaga gagacaacgt ttaagcatgt aaacctcttc
     7381 cttcaggctt taaatgagca ataaccttaa tgaatgtgac gatacattct ggaatggcag
     7441 tattctcggc tattggctga agtataccca tacccggaaa gagttactcc ttatttgccg
     7501 tgtggttagt cgctttacat cggtaagggt agggatttta cagcaccgtg aaaaatctca
     7561 taatttttat gaaatcactg tactcactat gggtaatgat aaatatcaat gatagataaa
     7621 gttatcttat cgtttgattt acatcaaatt gcctttagct acagacacta aggtggcaga
     7681 catcgaaacg agtatcagag gtgtctatga gtcactcatc cgcccccgaa agggctactg
     7741 gagctgtcat tacagattgg cgaccggaag atcctgcgtt ctggcaacaa cgcggtcaac
     7801 gtattgccag ccgtaacctg tggatttccg ttccctgtct gctgctggcg ttttgcgtat
     7861 ggatgttgtt cagcgctgtt gcggtgaact taccgaaagt cggttttaat tttacgaccg
     7921 atcagctatt tatgttgact gcgctgcctt cggtttctgg cgcgttatta cgtgttccat
     7981 actcctttat ggttcctatc ttcggtggtc gtcgctggac ggcgttcagc accggtattc
     8041 tgattattcc ttgcgtctgg ctgggttttg ccgtgcagga tacctccacg ccttatagcg
     8101 tcttcatcat catctctctg ctatgcggct ttgctggcgc gaacttcgca tccagtatgg
     8161 caaacatcag cttcttcttt ccgaaacaga agcagggtgg cgcgctgggt ctgaatggtg
     8221 gtctgggaaa catgggcgtc agcgtcatgc agttggttgc tccgctggtg gtatcactgt
     8281 cgattttcgc agtatttggt agccagggcg tcaaacagcc ggatgggact gagctgtatc
     8341 tggcgaatgc gtcctggata tgggtgccgt tccttgccat cttcaccatt gcggcgtggt
     8401 ttggcatgaa cgatcttgct acctcgaaag cctccatcaa ggagcagttg ccggtactca
     8461 aacggggtca tctgtggatt atgagcctgc tgtatctggc aaccttcggc tccttcatcg
     8521 gcttctccgc gggctttgca atgctgtcaa aaacgcagtt cccggatgtt cagattctgc
     8581 aatacgcttt cttcgggccg tttattggtg cgctggcgcg ttctgcaggt ggtgcattat
     8641 ctgaccgtct gggcggaact cgtgtcacgc tggtgaactt tattctgatg gcgattttca
     8701 gcggcctgct gttcctgacc ttaccgactg acgggcaggg cggaagcttc atggcgttct
     8761 tcgcggtctt cctggcgctg ttcctgacag ctgggctggg tagtggttcc actttccaga
     8821 tgatttcagt gatcttccgt aaactgacaa tggatcgcgt gaaagcagaa gggggttctg
     8881 acgaacgtgc gatgcgtgaa gcggcaaccg acacggcggc ggcgctgggt ttcatctctg
     8941 cgattggcgc gattggtggc ttctttatcc cgaaagcgtt tggtagctcg ctggcattaa
     9001 cgggttcgcc agtcggcgca atgaaggtat ttttgatttt ctatatcgcc tgcgtagtga
     9061 ttacctgggc ggtatatggt cggcattcta aaaaataaac cgttactcgt catactccgg
     9121 gttacatgtg ctgcggctgc gttcattcac cccagtcact tactttagta agctcctggg
     9181 attcattcac ttgccgcctt cctgtaaacc gaattatata gagtaaaata tttgattatc
     9241 ctttgcgcgg catgatgtcg cgcttttttt aagcgttatt tagttacaac atactaatgt
     9301 tatatggttt atttcgccgg atttcattaa gagccattaa tatgttaccc atggggaata
     9361 ctccttaata cccatctgca taaaaatctt aatagtttaa ataactacag gtataaaacg
     9421 tcttaattta cagtctgtta tgtggtggct gttgattatc ctaaaggggt atcttaggaa
     9481 tttactttat ttttcatccc catcactctt gatcgttatc aattcccacg ctgtttcaga
     9541 gcgttacctt gcccttaaac attagcaatg tcgatttatc agagggccga caggctccca
     9601 caggagaaaa ccgatgagta aattcctgga ccggtttcgc tacttcaaac agaagggtga
     9661 aacctttgcc gatgggcatg gccagcttct caataccaac cgtgactggg aggatggata
     9721 tcgccagcgt tggcagcatg acaaaatcgt ccgctctacc cacggggtaa actgcaccgg
     9781 ctcctgcagc tggaaaatct acgtcaaaaa cggtctggtc acctgggaaa cccagcagac
     9841 tgactatccg cgtacccgtc cggatctgcc aaaccatgaa cctcgcggct gcccgcgcgg
     9901 tgccagctac tcctggtatc tttacagtgc caaccgcctg aaatacccga tgatgcgcaa
     9961 acgcctgatg aaaatgtggc gtgaagcgaa ggcgctgcat agcgatccgg ttgaggcatg
    10021 ggcttctatc attgaagacg ccgataaagc gaaaagcttt aagcaggcgc gtggacgcgg
    10081 tggatttgtt cgttcttcct ggcaggaggt gaacgaactg atcgccgcat ctaacgttta
    10141 caccatcaaa aactacggcc cggaccgtgt tgctggtttc tcgccaattc cggcaatgtc
    10201 gatggtttct tacgcatcgg gtgcacgcta tctctcgctg attggcggta cttgcttaag
    10261 cttctacgac tggtactgcg acctgcctcc tgcgtctccg caaacctggg gcgagcaaac
    10321 tgacgtaccg gaatctgctg actggtataa ctccagctac atcatcgcct ggggttcaaa
    10381 cgtgccgcag acgcgtaccc cggatgctca cttctttact gaagtgcgtt acaaaggcac
    10441 caaaactgtt gccgtcacac cagactacgc tgaaatcgcc aaactgtgcg atctgtggct
    10501 ggcaccgaaa cagggcaccg atgcggcaat ggcgctggcg atgggccacg taatgctgcg
    10561 tgaattccac ctcgacaacc caagccagta tttcaccgac tatgtgcgtc gctacaccga
    10621 catgccgatg ctggtgatgc tggaagaacg cgacggttac tacgctgcag gtcgtatgct
    10681 gcgcgctgct gatctggttg atgcgctggg ccaggaaaac aatccggaat ggaaaactgt
    10741 cgcctttaat accaatggcg aaatggttgc gccgaacggt tctattggct tccgctgggg
    10801 cgagaagggc aaatggaatc ttgagcagcg cgacggcaaa actggcgaag aaaccgagct
    10861 gcaactgagc ctgctgggta gccaagatga gatcgctgag gtaggcttcc cgtactttgg
    10921 tggcgacggc acggaacact tcaacaaagt ggaactggaa aacgtgctgc tgcacaaact
    10981 gccggtgaaa cgcctgcaac tggctgatgg cagcaccgcc ctggtgacca ccgtttatga
    11041 tctgacgctg gcaaactacg gtctggaacg tggcctgaac gacgttaact gtgcaaccag
    11101 ctatgacgat gtgaaagctt ataccccggc ctgggccgag cagattaccg gcgtttctcg
    11161 cagccagatt attcgcatcg cccgtgaatt tgccgataac gctgataaaa cgcacggtcg
    11221 ttcgatgatt atcgtcggtg cggggctgaa ccactggtat cacctcgata tgaactatcg
    11281 tggtctgatc aacatgctga ttttctgcgg ctgtgtcggt cagagcgggg gcggctgggc
    11341 gcactatgta ggtcaggaaa aactgcgtcc gcaaaccggc tggcagccgc tggcgtttgc
    11401 ccttgactgg cagcgtccgg cgcgtcacat gaacagcact tcttatttct ataaccactc
    11461 cagccagtgg cgttatgaaa ccgtcacggc ggaagagttg ctgtcaccga tggcggacaa
    11521 atcccgctat accggacact tgatcgactt taacgtccgt gcggaacgca tgggctggct
    11581 gccgtctgca ccgcagttag gcactaaccc gctgactatc gctggcgaag cggaaaaagc
    11641 cgggatgaat ccggtggact atacggtgaa atccctgaaa gagggttcca tccgttttgc
    11701 ggcagaacaa ccagaaaacg gtaaaaacca cccgcgcaac ctgttcatct ggcgttctaa
    11761 cctgctcggt tcttccggta aaggtcatga gtttatgctc aagtacctgc tggggacgga
    11821 gcacggtatc cagggtaaag atctggggca acagggcggc gtgaagccgg aagaagtgga
    11881 ctggcaggac aatggtctgg aaggcaagct ggatctggtg gttacgctgg acttccgtct
    11941 gtcgagcacc tgtctctatt ccgacatcat tttgccgacg gcgacctggt acgaaaaaga
    12001 cgacatgaat acttcggata tgcatccgtt tattcacccg ctgtctgcgg cggtcgatcc
    12061 ggcctgggaa gcgaaaagcg actgggaaat ctacaaagcc atcgcgaaga aattctccga
    12121 agtgtgcgtc ggccatctgg gtaaagaaac cgacatcgtc acgctgccta tccagcatga
    12181 ctctgccgct gaactggcgc agccgctgga tgtgaaagac tggaaaaaag gcgagtgcga
    12241 cctgatccca ggtaaaaccg cgccacacat tatggtcgta gagcgcgctt atccggcgac
    12301 ttacgaacgc tttacctcta tcggcccgct gatggagaaa atcggtaatg gcggtaaagg
    12361 gattgcctgg aacacccaga gcgagatgga tctgctgcgt aagctcaact acaccaaagc
    12421 ggaaggtccg gcgaaaggcc agccgatgct gaacaccgca attgatgcgg cagagatgat
    12481 cctgacactg gcaccggaaa ccaacggtca ggtagccgtg aaagcctggg ctgccctgag
    12541 cgaatttacc ggtcgtgacc atacgcatct ggcgctgaat aaagaagacg agaagatccg
    12601 cttccgcgat attcaggcac agccgcgcaa aattatctcc agcccgacct ggtctggtct
    12661 ggaagatgaa cacgtttctt acaacgccgg ttacaccaac gttcacgagc tgatcccatg
    12721 gcgtacgctc tctggtcgtc agcaactgta tcaggatcac cagtggatgc gtgatttcgg
    12781 tgaaagcctg ctggtttatc gtccgccgat cgacacccgt tcggtgaaag aagtgatagg
    12841 ccagaaatcc aacggcaacc cggaaaaagc gctcaacttc ctgacgccgc accagaagtg
    12901 ggggatccac tccacctaca gcgacaacca gttgatgctg accttaggtc gcggtggccc
    12961 ggtggtctgg ctgagcgaag ccgatgccaa agagctgggc attgccgata acgactggat
    13021 tgaagtttac aacagcaacg gtgcgctgac tgcccgtgcg gttgtcagcc agcgtgttcc
    13081 ggcagggatg accatgatgt accacgcgca ggaacgtatc gttaacctgc cgggttcgga
    13141 aattactggc cagcgtgggg ggatccacaa ctcggtcacc cgtatcacgc cgaaaccgac
    13201 gcatatgatt ggtggctatg cccatctggc atacggcttt aactactacg gcaccgtagg
    13261 ttctaaccgc gatgagtttg ttgtagtgcg taagatgaag aacattgact ggttagatgg
    13321 cgaaggcaat gactaggtac aggagagcgt aaaatgaaaa ttcgttcaca agtcggcatg
    13381 gtgctgaatc tcgataagtg catcggctgc cacacctgtt cagttacctg taaaaacgtc
    13441 tggaccagcc gtgaaggcgt ggagtacgcg tggttcaaca acgtggaaac caagccgggt
    13501 cagggcttcc cgactgactg ggaaaaccag gaaaaataca aaggcggctg gatccgtaaa
    13561 atcaacggca aactgcagcc gcgcatgggt aaccgtgcca tgttgctggg taaaatcttc
    13621 gctaacccgc atctgccggg gatcgacgat tattacgagc cgttcgattt tgactatcag
    13681 aacctgcata ccgcgccgga aggcagcaaa tcgcagccga ttgcccgtcc ccgttcgctg
    13741 attaccgggg aacggatggc gaaaatcgaa aaagggccga actgggaaga tgacctgggt
    13801 ggtgagtttg acaaactggc gaaagacaag aacttcgaca acatccagaa ggcgatgtat
    13861 agccagttcg aaaacacctt tatgatgtat ttgccgcgcc tgtgcgaaca ctgcctgaac
    13921 ccggcatgtg tggcgacctg cccgagcggt gcgatttaca agcgtgaaga agatggcatc
    13981 gtcctgatcg accaggataa atgccgtggc tggcgtatgt gcatcaccgg atgcccgtac
    14041 aaaaaaatct acttcaactg gaagagcggt aagtctgaga agtgcatctt ctgctatccg
    14101 cgtattgaag cgggtcagcc gaccgtgtgc tcagaaacct gtgtcggtcg tatccgttat
    14161 cttggcgtgc tgctgtacga tgccgacgcg attgaacgtg cagccagcac cgagaacgag
    14221 aaagatcttt accagcgtca gctggaggtg ttcctcgatc cgaacgatcc gaaagtcatc
    14281 gagcaggcga ttaaagacgg tattccgctg agcgttattg aagccgcaca gcagtcgccg
    14341 gtttataaaa tggcaatgga atggaaactg gcgctgccgc tgcatccaga atatcgcaca
    14401 ctgccgatgg tctggtacgt gccgcctctg tctccgattc agtctgcagc agacgcgggt
    14461 gagctgggta gcaacggcat tctgccagac gtcgaaagct tgcgtattcc ggtacagtat
    14521 ctggcgaatc tgctgaccgc cggtgatacc aaaccggtac tgcgcgcact gaaacgtatg
    14581 ctggcgatgc gtcattacaa acgtgctgaa accgttgacg gtaaagttga tacccgtgcg
    14641 ctggaagagg tcggtctgac cgaagcccag gcacaggaga tgtaccgtta tctggctatt
    14701 gctaactacg aagatcgctt tgtggtgccg agtagtcatc gtgaactggc acgggaagcc
    14761 ttcccggaga aaaatggctg cggctttacc tttggtgatg gctgccacgg ctcagatacc
    14821 aaattcaatc tgttcaacag ccgtcgtatc gatgccatcg atgtgaccag caaaacggag
    14881 ccgcatccat gatcgaactc gtgattgtat cgcgtctcct tgaatatccg gatgctgcct
    14941 tatggcagca tcaacaagag atgtttgagg tgattgctgc gtcgaaaaat ctgtcgaaag
    15001 aagatgccca tgcgctgggc attttcctgc gcgatttaac ggcgatggat ccgctcgatg
    15061 cccaggccca gtacagcgaa ctgttcgacc gtggccgcgc cacgtcactg ttgctatttg
    15121 aacatgttca cggtgaatcc cgtgaccgcg gccaggcgat ggtggacctg ctggcgcagt
    15181 acgagcagca cggcttgcag ttaaacagcc gcgaattacc ggaccatctg ccgctgtatc
    15241 tggagtacct gtcgcagctg ccgcaaagcg aagccgtgga aggtttgaaa gatatcgcgc
    15301 cgattctggc attgctgagc gcgcgtctgc aacagcgtga aagccgttat gccgtgatgt
    15361 ttgatctgct gctgaaattg gccaataccg ctatcgacag cgacaaagtg gcggaaaaaa
    15421 ttgccgacga agcgcgcgat gatacgccgc aggcgctgga tgctgtctgg gaagaagagc
    15481 aggttaaatt ctttgctgac aaaggctgcg gtgattcagc aatcactgct catcagcgtc
    15541 gctttgccgg tgccgtcgcg ccgcaatatc tgaatatcac caccggagga cagcactaat
    15601 gcaattcctg aatatgttct tctttgatat ctacccgtac atagccgggg cggtcttcct
    15661 gattggtagc tggctgcgtt atgactacgg gcagtacacc tggcgcgcgg cgtccagcca
    15721 gatgctggat cgcaaaggga tgaacctggc gtcgaacctg ttccatatcg gtattctggg
    15781 gatttttgct ggtcacttct tcggtatgct gacgccgcac tggatgtatg aagcctggct
    15841 gccgattgaa gtgaaacaga aaatggcgat gtttgctggt ggtgccagcg gcgtgctgtg
    15901 tctgattggc ggcgtgctgt tgctgaagcg tcgtctgttc agcccacgcg tgcgtgcaac
    15961 cactaccgga gcggatatcc tgatcctgtc gctgctcgtt atccagtgcg cgctgggcct
    16021 gttgaccatt ccgttctccg ctcagcatat ggacggtagc gagatgatga aactggttgg
    16081 ctgggcgcag tcggtggtga ccttccacgg tggcgcttct caacacctcg atggtgtggc
    16141 gtttatcttc cgtctgcacc tggtgctggg gatgacgtta ttcctgctgt tcccgttctc
    16201 gcgtctggta cacatctgga gcgtaccggt ggagtatctg acacgtaagt accagctggt
    16261 gcgcgctcgt cactaaacga attttagttc acatagaccc tgcttcggcg gggtttttta
    16321 tgggcacggt gcggggtgag ttgtcggatg cgcttcgctt atccgaccta caggggagga
    16381 tattgtaggc ccggtaaacg tggtgccatc gggcaaaatt agctcggagt aacaggtttt
    16441 gattattcaa ggaggcgatg gtggtggggg aaggattact cagcgctgcg cgcttcgccc
    16501 ttcgggtcgt tgcctgcggc aacgctctct cgctggcgct caagtcgaac cttggtcgaa
    16561 gcttctcatc cttccccgct tgggcagaat atttaattgc ggattcgttt gagagtttac
    16621 ggacttttga aaagtgatgg tggtggggga aggattcgaa ccttcgaagt cgatgacggc
    16681 agatttacag tctgctccct ttggccgctc gggaacccca ccaggggtaa ttcaaatttt
    16741 gaggtaatgc ttgagatggt ggtgggggaa ggattcgaac cttcgaagtc gatgacggca
    16801 gatttacagt ctgctccctt tggccgctcg ggaaccccac cacggggtaa tgctttttac
    16861 tggcctgctc ccttatcggg aagcggggcg catcatatca aatgacgcgc cgctgtaaag
    16921 tgttacgttg agaaaaatga actggttgcg taattttcat ccgtaacgga ttaaaggtaa
    16981 ccagttattt ttgctggcga ttaaagaata atcgtccgat taccgtaaac aaagacgcgc
    17041 tgtgccagta ctttgtatag cgcacgactt aagacgtttt tctcgacgtc acgacctgcg
    17101 cgcatcatat cttcagctgt gtaggtatga tcgacatgaa taacgtcctg catgatgatt
    17161 gggccttcgt ccagattgtc attcacatag tgagcggttg cgccaataat cttcacacca
    17221 cgttcatagg cctggtgata aggacgtgcg ccgataaacg caggcaggaa tgaatggtga
    17281 atattgatga ttttattcgg gaagcgtgcc acaaattccg gcgttaatac ccgcatatac
    17341 ttcgccagca ccacatagtc aggtttataa gcatcaatgg cgtctgccat cttttgatcg
    17401 tgctcgttgc ggcttaaccc ttcatggttt accagctcaa acggaatatc aaaacgctca
    17461 accagagaac gtaaagtatc gtggttacca ataactgccg cgatttcgac atccaggccg
    17521 ccgtaattgg ctttcatcaa caaatcgcca aggcaatgcg cttctttagt gaccagaatc
    17581 actatccggc gacgaccggc aggattcagc tcacgcacgg agccttctgg caatgcgcta
    17641 tcgagatccg ccagcagggt ggaatcatta aaaatccctt ccagttccgt gcgcataaaa
    17701 aagcgcccgg tacggtgatc aacaaattca ttgttctgta cgatatttaa ctcgtgcttg
    17761 tagcaaatat tggtaatacg tgcgatcaga cctttttggt ccggacaaat agtacgcaga
    17821 actttacgtt ggagtgaatg cattgctgga aaaaccttgt tgagagtgtt tgctaaaccg
    17881 taccgtcaac cattattggc cgcagcactt tttaaatttt ttacctgaac cacaagggca
    17941 gggatcgttg cgaccaaact gcggacgtgt accgtcaata tagtaccatt gaccgttttc
    18001 tttcaaaaat cgcgaacgtt caattattgc acctgttttg ccaccttcgg taaatcgagc
    18061 aacaaaactg acaaatccga tattgtcggc gtcctgccaa caatgttcaa atacggtcaa
    18121 tcccaaccat tcagtatgcg caaatccggc catcaattcg gcacgtaacg ccgctgctcc
    18181 acaagaggga tgccaggtct ttattaaata atctgcgtct tgcatcacaa aagcgcagta
    18241 acgcgaacgc atgagatgtt ctggatcagg tgcaaccttt tcaccagaca cataagggtg
    18301 gcaacatagg ctatactcga cagcactacc acagggacaa agctgagaca caaataatct
    18361 ccctggaaac aataacggcg tattaaccgc ctgagtagca ctatgttaac cgagcagtag
    18421 cgatgtggct acgattgcat tccaggggaa tcttgcggga ataatgagaa agataaaaat
    18481 agggctggcg ctgggatctg gcgcggcgag aggttggtcg catattggcg ttattaatgc
    18541 gctaaaaaaa gtgggtattg aaattgatat cgttgcagga tgttcaattg gttcgctggt
    18601 gggcgctgcc tatgcatgcg atcgattatc tgcgctggaa gattgggtga cctctttcag
    18661 ttattgggat gttttacgcc tgatggatct ctcctggcag cgcggtgggt tactgcgcgg
    18721 cgagcgtgtc ttcaatcaat gccgcgaaat aatgccggaa acagagatcg aaaattgttc
    18781 ccgtcgcttt gcggctgttg ccaccaattt aagtacggga cgtgaattat ggtttactga
    18841 aggcgatctc catcttgcta ttcgcgcatc atgcagtatt ccaggactca tggcacctgt
    18901 tgcacataac ggctactggc tggttgatgg agcagtagtt aacccaattc ctatttccct
    18961 cacgcgtgta ttgggggctg atattgtgat agcggtcgac ctgcagcacg atgctcattt
    19021 gatacaacaa gatttgctct cctttaatgt cagtgaagaa aatagcgaga atggtgaatc
    19081 tttgccgtgg catgcgcgtc tgaaagaaag gttaggcagt ataacaacac gtcgggcggt
    19141 gacagcgcca acggcaacag agattatgac cacttctatc caggtgctgg agaaccgcct
    19201 taaaaggaac cgcatggcag gtgatccgcc cgatattctg attcaacctg tttgcccgca
    19261 aatatctacg cttgatttcc atcgcgcgca cgctgccatt gcggccggac agctggcagt
    19321 ggaaaggaaa atggacgaac ttttgccgtt ggtacgcacc aacatttgac cagaattttt
    19381 atctacactt aagttaattc tgacaggcgc aggtggcaat agcatgccac tattgagtaa
    19441 agccagtcag gggagagaac atgacgcagc cattggtcgg aaaacagatt ctcattgttg
    19501 aagatgagca ggtatttcgc tcgcttctgg attcatggtt ttcctcattg ggagcgacaa
    19561 cggtactggc ggctgatggg gtggatgccc ttgagttgct gggaggtttc actccagacc
    19621 tgatgatatg tgatatcgcg atgccacgaa tgaacgggct taaactgctg gagcatatac
    19681 gtaacagagg cgaccagacc ccagttctgg tgatatctgc cactgaaaat atggcagata
    19741 ttgccaaagc gttacgtctg ggcgttgaag atgttttgct gaaaccagtt aaagatctga
    19801 atcgcttgcg cgagatggtt tttgcctgtc tctatcccag catgtttaat tcgcgcgttg
    19861 aggaagagga aaggcttttt cgcgattggg atgcaacggt tgataaccct gccgcagcgg
    19921 cgaaattatt acaggaacta caaccgccgg ttcagcaggt gatttcccat tgccgggtta
    19981 attatcgtca attggttgcc gcggacaaac ccggcctggt gcttgatatt gccgcacttt
    20041 cggaaaacga tctggcattt tattgccttg atgtcacccg agctggacat aatggcgtac
    20101 ttgctgcctt gttattacgc gcattgttta acggattatt acaggaacag cttgcacacc
    20161 aaaatcaacg gttgccagag ctgggcgcgt tattgaagca ggtaaaccat ttactccgtc
    20221 aggccaatct gccggggcag tttccgctat tagttggcta ttatcatcgc gaactgaaaa
    20281 atctcattct ggtttctgcg ggtctgaatg cgacgttaaa taccggcgaa caccaggtgc
    20341 aaatcagtaa tggtgttccg ttaggcactt taggtaacgc ttatttgaat caattgagcc
    20401 agcgatgcga tgcctggcaa tgccaaatat ggggaaccgg tggtcgactg cgcttgatgt
    20461 tgtctgcaga atgagcaaac gataacgcgg actaaatttg cattacctgc taatgtcggc
    20521 tggtggtact atcgtcgcca ttcgtataag taattgtctt aattatgcta actcgcctcc
    20581 ttttcagaac ttagcccctt cggggtgctg atatactggg atgcgataca gaaatatgaa
    20641 cacgttcaaa acacgaacag tccaggagaa tttaaatggc tgccattaat acgaaagtca
    20701 aaaaagccgt tatccccgtt gcgggattag gaaccaggat gttgccggcg acgaaagcca
    20761 tcccgaaaga gatgctgcca cttgtcgata agccattaat tcaatacgtc gtgaatgaat
    20821 gtattgcggc tggcattact gaaattgtgc tggttacgca ctcatctaaa aactcgattg
    20881 aaaaccactt tgataccagt tttgaactgg aagcaatgct ggaaaaacgt gtaaaacgtc
    20941 aactgcttga tgaagtgcag tctatttgtc cgccgcacgt gactattatg caagttcgtc
    21001 aggggctggc gaaaggcctg ggacacgcgg tattgtgtgc tcacccggta gtgggtgatg
    21061 aaccggtagc tgttattttg cctgatgtta ttctggatga atatgaatcc gatttgtcac
    21121 aggataacct ggcagagatg atccgccgct ttgatgaaac gggtcatagc cagatcatgg
    21181 ttgaacaggt tgctgatgtg accgcatatg gcgttgtgga ttgcaaaggc gttgaattag
    21241 cgccgggtga aagtgtaccg atggttggtg tggtagaaaa accgaaagcg gatgttgcgc
    21301 cgtctaatct cgctattgtg ggtcgttacg tacttagcgc ggatatttgg ccgttgctgg
    21361 caaaaacccc tccgggagct ggtgatgaaa ttcagctcac cgacgcaatt gatatgctga
    21421 tcgaaaaaga aacggtggaa gcctatcata tgaaagggaa gagccatgac tgcggtaata
    21481 aattaggtta catgcaggcc ttcgttgaat acggtattcg tcataacacc cttggcacgg
    21541 aatttaaagc ctggcttgaa gaagagatgg gcattaagaa gtaacatcca tatcggtgtt
    21601 atccacgaaa cggcgttgaa caatcgacgc cgttttttta tagcttattc ttattaaatt
    21661 gtcttaaacc ggacaataaa aaatcccgcc gatggcggga tttttagcaa ttgcaatcta
    21721 caaaagatta ttgcttgatc aggaaatcgt cgagggattt accttgctca tccattgctt
    21781 ttttgattac agctggagta cggccctggc cagtccaggt tttagtttcg ccgttttcgt
    21841 caacatagct atattttgcc ggacgctgag cacgtttagc tttggtgccg gatttaacgg
    21901 cagcaaggct attcagcagt tcgttcgggt caataccgtc agcgatcagc atttcgcgat
    21961 attgctgcag tttacgagtg cgctcttcaa cttcagcagc agccgcgctt tcttcttcgc
    22021 gacgttcgtt aacaacaact tctaattttt ccagcatttc ttccagcgtt tcaagtgtac
    22081 attctcttgc ctgcgcacga agagtacgga tgttgttcag aattttaagt gcttcgctca
    22141 ttgtagtaat ctcaaactta tattggggtg gtttgttgag gtaataatag agccttaaat
    22201 tcagttgtgc aatagccagg aatgtaagga attcaaaatt gttctttatt ttgtgccgcc
    22261 aataaatatc ttttcataaa attagccaga aaagacgcgg catatagccc tatttacacc
    22321 gatgatttcg cagcacgtga ggttaaaact tcctgattca tgtcacattt tatggggaga
    22381 ttatcgtagg ctgacggcct ttcagtcttc tgtattagtt gtgtttacga gaattcccta
    22441 ttaagcgaat gatgaaaagt agaacagtcg caataagagc atggacttag tattgcacta
    22501 tctcctggag gtcaacagag ggctattact tgcgcaacag gttaaagatt gtgaatagtt
    22561 accagcagtc atttacccgc ttataacaag cgaggcagtt gtaatgatag ctcagaagga
    22621 ttatgcaagg cttcgtaagg gagaacgcat atacccactt ctgtgcatac tgttgagctg
    22681 aaaaactgac gaattatgat aaactccagc caactttatt tcatatcatt gagggcctgt
    22741 ggctgatggc acagctatat ttctactatt ccgcaatgaa tgcgggtaag tctacagcat
    22801 tgttgcaatc ttcatacaat taccaagaac gcggcatgcg cactgtcgta tatacggcag
    22861 aaattgatga tcgctttggt gccgggaaag tcagttcgcg tataggtttg tcatcgcctg
    22921 caaaattatt taaccaaaat tcatcattat ttgatgagat tcgtgcggaa catgaacagc
    22981 aggcaattca ttgcgtactg gttgatgaat gccagttttt aaccagacaa caagtatatg
    23041 aattatcgga ggttgtcgat caactcgata tacccgtact ttgttatggt ttacgtaccg
    23101 attttcgagg tgaattattt attggcagcc aatacttact ggcatggtcc gacaaactgg
    23161 ttgaattaaa aaccatctgt ttttgtggcc gtaaagcaag catggtgctg cgtcttgatc
    23221 aagcaggcag accttataac gaaggtgagc aggtggtaat tggtggtaat gaacgatacg
    23281 tttctgtatg ccgtaaacac tataaagagg cgttacaagt cggctcatta acggctattc
    23341 aggaaaggca tcgccacgat taataagaat ttctttactg acagggtgag cagggcactt
    23401 ttatcctgtc agttcgtttt acgcacttct tccgggctat atacccttct cggcagtttt
    23461 ttaacgccgc tatacgcctc acagggctct taagcaccga cgttgacttg tgacctgtaa
    23521 agtacaatat ccctgtgttt aggcgttata catcgtcgca aatatgatga aggctaatgc
    23581 tgtcggttta tggaaaagtt gctttgggta aacaaaaaat acggccccag aagggcaatg
    23641 ccgttcactt aagaggagcg gcactatgtt tcacaggata acgggttttt gatatcttaa
    23701 ccgacctcgg ccttgatggt cgggggcgtt ttgttatgaa caccacttcc agagcacccc
    23761 gaagatgctc cagtcgtttc gggatggtcc ctggtgacgc cgtgtttccc agctctatca
    23821 tttctgatgc gatattcttc cacgcaggca gtagccagtg gcggttacaa cccttctggt
    23881 tcagcgttag cagcaggtct tcgctgtaaa aaagcttatc aaacaacgta atagagttat
    23941 ccgggatggt ggcgagcatg gagtgggcca gcacagtttc gctctgccgg taaggtgcgg
    24001 tcacggcatt cagcagaatg tgacttccca ggttcattaa ggccaccaga cgcataaccg
    24061 ggtaggcgtt ctgccgctta gtggatgtgt tggcagaccc ataatattca cgcagctcgg
    24121 gtttatcagg tgtcctgaac tgtgcgccat caatggcaaa aagttgcagg ccgtgccagt
    24181 catccttcag gtaacgttcc gcgcccctgt ctgtgcggtc tggcggaaga gccattccac
    24241 tggggcggcc cccacgcgct gacgcgcttg ggtgacagcg ctgcgggcca gcaggttcat
    24301 ccccgcttcg ccatccgcgc tcaggttcag acggcgaaca acatcggtaa ttggctcatt
    24361 gcaccaccat ccagataacc atgtcccccg gtaaacgacg gcggcgaacg gtcgcatgag
    24421 cagaaagcgt caggcagtgt tgtatccact cggtgggaag gtgttctgca aatagttgtg
    24481 cagagggcgg aggcataagc ggatggtcac tgaaatcgag cagatcattg agaagtggca
    24541 taagaaaacg gctccctgtt gtggaagccg ttatagtgcc tcagtttaag gatcggtcaa
    24601 ctaatcctta actgatcggc attgcccaga aggggccgtt tatgttgcca gacagcgcta
    24661 ctgattaagc ggattttttc gcttttttct cagctttagc cggagcagct tctttcttcg
    24721 ctgcagtttc accttctaca taatcacgac cgtagtaggt atccagcaga atctgtttca
    24781 gctcggagat cagcgggtaa cgcgggttag cgccggtgca ctggtcatcg aatgcatctt
    24841 cagacagttt atccacgttc gccaggaagt ctgcttcctg aacgccagct tcacggatag
    24901 atttcggaat acccagttca gctttcagcg tttccagcca tgccagcagt ttctcgatct
    24961 tagcagcagt acggtcgccc ggtgcgctca gacccaggtg gtcagcaatt tcagcataac
    25021 gacggcgagc ctgcggacgg tcatactggc tgaatgcagt ctgcttggtc gggttgtcgt
    25081 tcgcgttgta gcgaataacg ttacaaatca gcagggcgtt tgccagaccg tgcggaatat
    25141 ggaactggga acccagtttg tgcgccattg agtgacatac acccaggaag gcgttcgcaa
    25201 acgcgatacc cgcgatagtc gctgcactgt gaacacgttc acgcgctacc ggatttttag
    25261 acccttcgtg gtaggacgct ggcagatatt ctttcagcag tttcagtgcc tgcagagcct
    25321 gaccatcaga gaactcagat gccagtacag aaacataagc ttccatggcg tgagttactg
    25381 cgtccagacc accgaaagca cacagggact tcggcatgtc cataaccagg ttggcgtcga
    25441 caatcgccat atccggagtc agcgcatagt ctgccagcgg atatttctga ccagtagcgt
    25501 cgtcagttac aaccgcaaac ggagtgactt cagaacctgt accagaagtg gtggtgacag
    25561 cgatcatttt cgctttcacg cccattttcg ggaacttgta gatacgttta cggatatcca
    25621 taaagcgcag cgccagctct tcgaagtgag tttccggatg ttcgtacata acccacatga
    25681 tcttcgcggc gtccatcggg gaaccaccac ccagcgcgat aatcacgtct ggtttgaagg
    25741 agtttgccag ttctgcacct ttacgaacga tgctcagggt cgggtccgct tctacttcga
    25801 agaagacttc agtttcaacg cctgctgctt tcagtacgga agtgatctga tcagcataac
    25861 cattgttgaa caggaagcgg tcagtcacga tgagcgcacg tttgtggcca tcagtaatca
    25921 cttcatccag cgcgattggc agggagccac ggcggaagta gatagatttc ggaagtttgt
    25981 gccacaacat gttttcagct cgcttagcaa cggttttctt gttgatcagg tgtttcggac
    26041 caacgttttc agagatggag ttaccacccc aagaaccaca acccagagtc agggaaggtg
    26101 cgagtttgaa gttatacagg tcaccgatac caccctgaga cgctggggtg ttaatcagga
    26161 tacgagccgt tttcattttc tgaccgaagt aagaaacgcg agccggttgg ttatcctggt
    26221 cagtgtacag gcaagaggta tgaccgatac cgcccatagc aaccagtttc tctgcttttt
    26281 ctaccgcgtc ttcgaaatct ttagcgcggt acattgccag agtcggggac agtttttcat
    26341 gtgcgaacgg ttcgctttca tcaacaacgg tcacttcacc gatcagaatc ttggtgtttt
    26401 ctggtacaga gaagcctgcc agttcagcaa ttttataggc tggctgacca acgatagccg
    26461 cgttcagcgc accgtttttc aggataacat cctgaacagc tttcagctct ttaccctgca
    26521 acagatagcc gccgtgggtt gcaaaacgtt cacgtacagc gtcataaaca gagtcaacaa
    26581 caacaacaga ctgttcagaa gcacagatta cgccgttgtc gaaggttttg gacatcagta
    26641 cagatgcaac tgcacgtttg atatcagcag tttcatcgat aacaactgga gtgttgcccg
    26701 cgcctacacc gatagctggt ttaccggagc tgtatgcggc tttaaccatg cccggaccac
    26761 cagtcgcgag gatcaggttg atgtctgggt ggtgcatcag tgcgttagac agttcaacag
    26821 aaggttgatc gatccagccg atcagatctt tcggagcacc ggcagcgata gcagcctgca
    26881 gaacgatatc agccgctttg ttggtggcat cttttgcacg cgggtgcggg gagaagataa
    26941 tggcgttacg ggtcttcaga ctgatcagcg atttgaagat agcagttgaa gtcgggttag
    27001 tggtcggaac gataccgcaa ataataccga ttggttcagc gatagtgatg gtaccaaaag
    27061 tgtcgtcttc agacagaata ccacaggttt tttcatcttt ataggcgttg tagatatatt
    27121 cagaagcaaa gtggtttttg atcactttat cttcgacgat acccatgccg gattcggcaa
    27181 cggccatttt cgcgagtggg attcgagcat ctgcagcagc cagagcggcg gcgcggaaga
    27241 ttttgtctac ttgctcttga gtgaaactgg catattcacg ctgggctttc tttacacgct
    27301 ctacgagtgc gttaagttca gcgacattag taacagccat aatgctctcc tgataatgtt
    27361 aaactttttt agtaaatcat ctgctcgaat acgagagtat agtcagtgcg gtgatgattt
    27421 gcttaaccta tgaaaatcaa aagcttactc gcgctcacac tcactgtgat ttactaaaag
    27481 agtttaaaca ttagagttat tatctctaat gcgtcacttc caggtggcgt aagcaagatt
    27541 actcacttct gggtactgat tacgtgatcc aaatcaaatt ttttgcaaag ctgacacctt
    27601 tcagcatcgc ttttcgccat tatagctaac agttaataaa ttgtagtatg atttggtggc
    27661 tacattagca tgttttgcac aactagataa caataacgaa tgatagcaat tttaagtagt
    27721 taggaggtga aaaatgctgt caaaaggcgt attgtcagcg cgtcttttca accttattta
    27781 tggctaacat tatccggctt ttgcttcgga gctaaccgtg attcagacct tttttgattt
    27841 tcccgtttac ttcaaatttt tcatcgggtt atttgcgctg gtcaacccgg tagggattat
    27901 tcccgtcttt atcagcatga ccagttatca gacagcggca gcgcgaaaca aaactaacct
    27961 tacagccaac ctgtctgtgg ccattatctt gtggatctcg ctttttctcg gcgacacgat
    28021 tctgcaactt tttggtatat caattgattc gttccgtatc gccgggggca tcctggtggt
    28081 gacgatagcg atgtcgatga tcagcggcaa gcttggcgag gataaacaga acaagcaaga
    28141 aaaatcagaa accgcggtac gtgaaagcat tggtgtggtg ccattggcgt tgccgttgat
    28201 ggcggggcca ggggcgatca gttctaccat cgtctggggt acgcgttatc acagcattag
    28261 ctatctgttt ggtttctttg tggctattgc attgttcgct ttatgttgct ggggattgtt
    28321 ccgcatggca ccttggctgg tacgggtttt acgccagacc ggcatcaacg tgattacgcg
    28381 tattatgggg ctattgctga tggcattggg gattgaattt atcgttactg gtattaaggg
    28441 gattttcccc ggcctgctta attaattcct ttcaaatgaa acggagctgc catgctccgt
    28501 ttacttcgtc attattttta ctttgttccc g
//
LOCUS       DAJTMC010000035        28223 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.035, whole genome shotgun sequence.
ACCESSION   DAJTMC010000035 DAJTMC010000000
VERSION     DAJTMC010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28223
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.035"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(1..777)
                     /locus_tag="OO130_001369"
                     /pseudo
     CDS             complement(1..777)
                     /locus_tag="OO130_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE9 sensor domain-containing protein"
     gene            complement(955..1866)
                     /gene="gsiD"
                     /locus_tag="OO130_001370"
     CDS             complement(955..1866)
                     /gene="gsiD"
                     /locus_tag="OO130_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HCR5364838.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(1869..2789)
                     /gene="gsiC"
                     /locus_tag="OO130_001371"
     CDS             complement(1869..2789)
                     /gene="gsiC"
                     /locus_tag="OO130_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HCR5364839.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILSSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(2807..4345)
                     /gene="gsiB"
                     /locus_tag="OO130_001372"
     CDS             complement(2807..4345)
                     /gene="gsiB"
                     /locus_tag="OO130_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HCR5364840.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAAAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILVH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQAALLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(4365..6236)
                     /gene="gsiA"
                     /locus_tag="OO130_001373"
     CDS             complement(4365..6236)
                     /gene="gsiA"
                     /locus_tag="OO130_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HCR5364841.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLQRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQEPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLCVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(6223..7188)
                     /gene="iaaA"
                     /locus_tag="OO130_001374"
     CDS             complement(6223..7188)
                     /gene="iaaA"
                     /locus_tag="OO130_001374"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCR5364842.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFARGMERVSPAIFSTPLRYEQLLAARKEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIGHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            7392..8627
                     /gene="moeA"
                     /locus_tag="OO130_001375"
     CDS             7392..8627
                     /gene="moeA"
                     /locus_tag="OO130_001375"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HCR5364843.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVGEWVEVEPFNALFGGL"
     gene            8627..9376
                     /gene="moeB"
                     /locus_tag="OO130_001376"
     CDS             8627..9376
                     /gene="moeB"
                     /locus_tag="OO130_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HCR5364844.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLIVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHNDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEYDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(9452..10114)
                     /gene="fsa"
                     /locus_tag="OO130_001377"
     CDS             complement(9452..10114)
                     /gene="fsa"
                     /locus_tag="OO130_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HCR5364845.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKNPRQALDCLLAGCESITLPLDVAQQMISYPAVDAAVAKFEQDWLGAFGRT
                     SI"
     gene            10245..11144
                     /locus_tag="OO130_001378"
     CDS             10245..11144
                     /locus_tag="OO130_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HCR5364846.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEIATALLQACHEAGIHTAVETC
                     LHVPWKYITPSLPYIDLFLADLKHIADAPFKQWTDGNAAKVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APDLLDFAQQYACQKGLTATLRG"
     gene            11150..13582
                     /locus_tag="OO130_001379"
     CDS             11150..13582
                     /locus_tag="OO130_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HCR5364847.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLAIAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQTLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGNVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            13728..14543
                     /gene="ybiV"
                     /locus_tag="OO130_001380"
     CDS             13728..14543
                     /gene="ybiV"
                     /locus_tag="OO130_001380"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HCR5364848.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIEFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKAVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIAFDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            14695..15960
                     /locus_tag="OO130_001381"
     CDS             14695..15960
                     /locus_tag="OO130_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HCR5364849.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFSREQALGW
                     DQSMLDYLDRNRFDEVYKGPGDNFFGTLSTSRPEIYPIYWSQTQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQ
                     RVFANVFNGNLAQYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     HEDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(16201..17793)
                     /locus_tag="OO130_001382"
     CDS             complement(16201..17793)
                     /locus_tag="OO130_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HCR5364850.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLMKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            18012..18932
                     /gene="ldtB"
                     /locus_tag="OO130_001383"
     CDS             18012..18932
                     /gene="ldtB"
                     /locus_tag="OO130_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR5364851.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(18991..20109)
                     /locus_tag="OO130_001384"
     CDS             complement(18991..20109)
                     /locus_tag="OO130_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HCR5364852.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKVLQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     FKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLASLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVILPAN"
     gene            complement(20106..20573)
                     /gene="mntR"
                     /locus_tag="OO130_001385"
     CDS             complement(20106..20573)
                     /gene="mntR"
                     /locus_tag="OO130_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HCR5364853.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            20759..20887
                     /gene="mntS"
                     /locus_tag="OO130_001386"
     CDS             20759..20887
                     /gene="mntS"
                     /locus_tag="OO130_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HCR5364854.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLILLSMLCDMVNNKPQQDKPSDK"
     gene            21159..22742
                     /gene="opgE"
                     /locus_tag="OO130_001387"
     CDS             21159..22742
                     /gene="opgE"
                     /locus_tag="OO130_001387"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HCR5364855.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(22791..23306)
                     /gene="ompX"
                     /locus_tag="OO130_001388"
     CDS             complement(22791..23306)
                     /gene="ompX"
                     /locus_tag="OO130_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HCR5364856.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(23359..23424)
                     /locus_tag="OO130_001389"
     CDS             complement(23359..23424)
                     /locus_tag="OO130_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5364857.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            23659..24546
                     /gene="rhtA"
                     /locus_tag="OO130_001390"
     CDS             23659..24546
                     /gene="rhtA"
                     /locus_tag="OO130_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HCR5364858.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            24845..25348
                     /gene="dps"
                     /locus_tag="OO130_001391"
                     /gene_synonym="pexB"
     CDS             24845..25348
                     /gene="dps"
                     /locus_tag="OO130_001391"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HCR5364859.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            25752..26498
                     /gene="glnH"
                     /locus_tag="OO130_001392"
     CDS             25752..26498
                     /gene="glnH"
                     /locus_tag="OO130_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HCR5364860.1"
                     /translation="MKSVLKVSLAALTLVFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            26637..27296
                     /gene="glnP"
                     /locus_tag="OO130_001393"
     CDS             26637..27296
                     /gene="glnP"
                     /locus_tag="OO130_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HCR5364861.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            27293..28015
                     /gene="glnQ"
                     /locus_tag="OO130_001394"
     CDS             27293..28015
                     /gene="glnQ"
                     /locus_tag="OO130_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HCR5364862.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     NPQVLIKNPPSQRLQEFLQHVS"
BASE COUNT         6980 a         7540 c         6970 g         6733 t
ORIGIN      
        1 tgtctttgca atatactccg tatcgacaga atcaataata ttataattac cttcgctatt
       61 ataaataatc agtttttcac gagcgagttg attcttggca atgatctcat gaccttgttt
      121 caaaaataga acttcatcat tagcgctgat ttgatcaaga tactcgttca aataccgcca
      181 gtttaaatcg acatgaattt tatctaccgc taacaaatcg tgatctttgc cgttgatacg
      241 tagcgtaaaa gagatacatt ttttacccgg acgatcaaag caattgtatg ccggattcca
      301 gctgttgtta gagtcatctg aagccaccga ccacggtcgt ttcgatgggt taaaatcggc
      361 aaaaataacc cgccctgact cgtcgacaag cggtccattt acttattcct gatgataaac
      421 aacgatttta tcattcgccc tccgtgaaaa gagataatat ttattatcat caagtaccag
      481 tcccaccgag tctaaaaatg gcatcatttc cattttcaga atcacggcac gttgaatctc
      541 ctctgtattt tttaataaat acagtgagtc ctttaatgtc agggccgaac gttcaatatc
      601 tttaaagaaa tagccaacgt ttttctgaat aagttcaaag cggctatcgg cacgttttac
      661 cgcgcgttcc gataccagtt gatagttaat gatgaaaaaa agaccaataa ataacagtgc
      721 tgccaataaa aataaaatta tcagccttat ctttatcttt tcgtataaac tcagcatgtt
      781 aatacattta acaaaataac tatctgaacg ctgtgccgct gcaaaaatag tcccttgctg
      841 cggcttttta accaaattac ccgattctac aaaatcagca gaaagttaca acacttgctt
      901 agggcaataa tttcctgtga ggtgattacc ctttcaagca atattcaaac gtaactatcc
      961 tttaattttc ggatccagcg catcgcgtaa accatcgccc aacaaattga acgccagtac
     1021 ggtcagaaaa atagccaggg ccggaaaaac agcgacatgc ggcgcgataa ccatatccgc
     1081 tcgtgcctca ttgagcattg ctccccactc tggtgtcggc ggctgcgcac cgaggccgag
     1141 aaatgacagg ctggcggcag agataatcga ggtgccaatg cgcatggtga aaaacaccac
     1201 gatagacgag acggtcccag gcaagatatg acgcaataaa atggtcatat cgctggcacc
     1261 aatactgcgt gctgactcaa taaaggtttg ctgtttcaac accagcgtgt tgccgcgcac
     1321 cagtcgggca aacgcgggga tggaaaaaat ggcgactgcg ataatcacgt tagcaatgcc
     1381 gcttcccaac accgcaacaa cagcgatcgc cagtaaaata cccgggaagg caaacagcac
     1441 atcgcaaatg cgcatgatca gccgatccca ccagccttca taatatccag ccaacaagcc
     1501 cagcaacgtc ccgatcgccg caccgataaa cacggcaaac acgcccgccg ccagcgagat
     1561 ttgcgcacca accaggacac ggctgaaaat gtcgcgcccc agtgaatcga cgccaaacca
     1621 gtgctgcaaa gaaggtccgt tattcagatt gtcataatca aaataatttt cggcgtcata
     1681 gggagcgatc cagcgtgcaa aaatggccac cacaatcaat aaaataacga ataatgcggc
     1741 ggtcatcgcc atatgctgac ggcgaaatcg tcgccagaat tcatgccacg gtgtacgcac
     1801 ctggtcaggt ttgaccagtg gcatggcgtt taacaccgcc tgacgtcgcc agttaaatag
     1861 tcgcatcctt acttgtacct gatagccggg ttaatggcgg cgtaaagcac atccaccact
     1921 aagttgataa gaataaattc cagcgagaaa agcagaattt ccgcctgaat caccgggtaa
     1981 tcgcgcattt ctacggagtc aacgagtaag cgtccaagtc cgggccagtt gaaaactttc
     2041 tccaccacga tagaaccacc gagcaaaaag ccaaactgta agcccatcat ggtcactacc
     2101 gggatcatcg cgttacgtag cccgtgtttg aggacaaccc aggtttcgct cacccctttc
     2161 gccctcgcgg tacgcatata atcttcgctt aaaacatcga caaacgacgc gcgggtaaag
     2221 cgcgccatca cggctgccac tgccgcgccg agcgtcaggg aggataaaat gtagtgctgc
     2281 caactgtctg ctcccacggt aggcagccag cccagttcaa cggagaatac ctgaattaaa
     2341 agcatcccca gcgcaaatgc cggaaacgag atccccgaca ccgcaatggt catactcaat
     2401 cgatccggcc aacgattacg ccagacggcg gcgataattc ccgccgccat accaaatatc
     2461 accgcccaga ccatactggt tatggtcagc cacagcgttg gcataaagcg gctggcaatc
     2521 tcatcggcaa ccggacgacg cgacaccatc gacaggccaa aatccccctg cacagcattg
     2581 ctgatatagt gccagaactg gtgatacagc ggctgatcca accccagctg ctgacgcacc
     2641 agttctataa cctgcgcatc agcttcgggc ccggcaatca atcgcgccgg atcgccgggc
     2701 agcatatgca caaataaaaa caccagcacc gagacgataa acagcgtcgg aatcaacccc
     2761 agtaagcgtt tgataacgta attaagcatt ccactccctg cgttgcttat tgcaaatccg
     2821 cgtcttcaaa gctgaagccg gtgtctggca tgatccaaaa accggtcagg tttttactgt
     2881 gtgccgacac cagtttttct accaccagcg ggatccacgg cgactcttgc cagatgatat
     2941 cctgcgccgc cttatacaag cgggtctttt ccgccggatc attagttttc agtgcctgag
     3001 ccaggaagtc gtccacctgt ttattgctgt aaaatgcggt attaaacagc gtcggtggcc
     3061 agttttgcga ggcgaacagt ggcgatagcg cccagtccgc ttcgccggtt gaagccgacc
     3121 agccagtgta gaacatccgc acgccgctct ctttttgccc tttaccttca acttctgccg
     3181 cccgctgtcc ggcatccatc gcggtcacct gggctttaat cccgacctgc gctaactgct
     3241 gctgggtaaa ttgcagcact ttctgcgcgg tgctgtggtt atgtgacgac cacagcgtgg
     3301 tactgaaacc gttgggatat cccgcctctt tcagtaattc gcgcgctttc actggatcgt
     3361 aaggccacgg tttataactt tgcgcgtagg cgatacttgg cggtaccaca ccagtagctg
     3421 gcgttgcata gcccgcaaag gcaactttca ccagcgccgg acggttaatg gcgtaattca
     3481 gcgcctcacg gaccttcggg ttatcgaacg gcttttgcgt cacgttcata ctgatataac
     3541 gctgcataat tgacggactg gccatcaact cgatattttt gtttttctcc agcagtgcgg
     3601 cctgctcgta gggaatgggg aaagcaaact gcgcttcacc ggtttgcagc attgccgcgc
     3661 gggtgttgtt atccgccacc ggacgccagg ttatgctgtc cagtttgggc aatcctggct
     3721 gccagtaacc cgcgaatttt ttcaccttca caaaatcggt ctgattccag gtatccagtt
     3781 catacggtcc ggttcccacc ggatgaaaac caatctcctt gccatatttt tccagcgctg
     3841 ccggtgaaat catcgcggtc gccggatgga caagaatatt aataaacgct gagaacggct
     3901 gtttgagggt aatctttacc gttgtcggat cgatcgcctc cgttttggca atattcttat
     3961 acaggttata gcgtttaaga tgattcgccg gatcgctggc ccggtccaga ttcgctttca
     4021 ccgccgcggc gttgaaatcg gtgccatcct ggaatttaat tccttcccgc aatttcacgg
     4081 tgtaagtaat gccgtcatcg gaaacggtat aactctccgc cagcacgttt ttcagtttca
     4141 tctctttatc cagaccgaac agcccctggt aaaacgattt cgctacggcc tgagataacg
     4201 tgtcattcgc gtcatacgga tcgagcgtgg tgaaattcga tcctaccgcc accaccacat
     4261 ctttggcagc gaatgcacaa gatgccatca acgctgtcgc aatgcccagc gccactaacc
     4321 cactacggtg tacagctctt gccattttat tctccgcctg aatgttatct acgcatgaat
     4381 gcgtattctg attgtggttg tgcgacgtaa tgccccggcc cgacgcattg caacgagacg
     4441 gctgccacct cttcgccacg cagatgaata ttgctgggaa gatcgtccga cagcagtaca
     4501 cgctgcggtc gttgtcggga cggttcagcg accggaactg ccgccagtaa tttacgcgta
     4561 taaggatgct gcgggttttc gaagaccgcg cgccgtggac caatttcaac aatttgcccg
     4621 agatacatca ccgccacacg atgactaatc cgctctacca cggccatatc gtgggagata
     4681 aacagatacg caatgccgaa atcacgctgg agatcgagca acaagttgat aatctgcccg
     4741 cgaatagaaa catccagcgc cgaaacggct tcgtcggcaa tgatcacttt tggattcaat
     4801 gccaacgcgc gagcaatgca gatgcgctgg cgctgaccgc cggaaaactc atgcgggtaa
     4861 cgccaggcat gttcaggtaa caggcccacg cgctccagca accacgcaac gcgtgcagcc
     4921 gcgtctttac ctggcaataa accgtgtaca cacagcggtt cgataatcga atcaccgatg
     4981 gtctgacgtg ggtccagcga agcgtaaggg tcctgaaaaa taaactgaat atcccggcgc
     5041 aatgcctgaa gtttgccagg tgacaaggta tcgattcgct gaccgttaaa gataatttcg
     5101 ccgccctgcg attcgaccag gcgcagcaac gcccgcccgg tagtggattt accgctgcca
     5161 gactcgccca ccagcgatag cgtttcgcca ggccagagat caaaactgac tttctcaacg
     5221 gcatgcactt cccgcgttac gcgattcaac aaaccgctgc gcaaagggaa acgggtgaca
     5281 agattacgca ctcgtaaaac aggttcgcca tccaccaccg ttttctgctc gatggggggt
     5341 tcctgtttcg ctggatgttc aagcgatatc aatgggaaac gtcggggata atctaaccct
     5401 ttcatcgcac caagttgcgg aacagcagct aacagcgcac gggtgtaagg atgttgcggt
     5461 gcatgaaaaa tctgttcgac ggtacccgtt tccaccgcct cgccctgata catcaccagt
     5521 acccgatcgg ctatctctgc caccacgccc atatcgtgcg tgataaagat aacgcccatc
     5581 gacatctctt tttgcaatac tttgattaat tgcaggatct gcgcctgaat agtgacatcc
     5641 agcgcggtgg ttggctcatc ggcaatcagc accgccgggc ggcatgacag cgccatcgca
     5701 atcatcactc gctggcgcat cccgccagag agttgatgcg gataacgtga aagaatggtt
     5761 tgtgcctcag gaatgcgtac ctgatccagc atccgcttcg cctcgaccat cgcttcttca
     5821 cgactggcgt tctgatgcag acgaattgat tcggcaatct gttcacccac agtaaatacc
     5881 gggttcagcg atgtcatcgg ctcctgaaat atcatcgcca tatccgcacc gcgaacatgg
     5941 cgcatttgtg cagcgctctg ctcgctaagt tcaatcactt cgcgactgcg ccgctgcaac
     6001 agcattttat cgcactgtac taaaccgccc gcctgttcca acaggcgcat caatgccaac
     6061 gcagtcactg acttaccgga gccggattcg ccaacaattg ccagcgtctc accgcgttgc
     6121 agactaaaag agagattgcg gaccgcagct attttctgct ggtcctgcat aaaggcaata
     6181 ttcagatttt caaccgccag cacattaccg gcatcaagtt catcactgtg tggcaacggt
     6241 gtcccctttt tcacggtaga taccggtggt tggcgtatcg cctgcgtagc cccaggcgcg
     6301 atacattcct tcggtgttaa acggtagcgc gacattccct tcatggccga tagcgattaa
     6361 gccaccgcta ccgccaagcg cagggagttt ttccattact acccgctcgc aggcttccgc
     6421 gagacttaat ccgccgtaat ccattaacgc ggcgatgtca tatgccgcca gcgcgcggat
     6481 gaagacttcg cccgtgccgg tacaagaaac cgccacactg gcgttattgg cgtagcatcc
     6541 ggcacccact aagggactat cgccaactcg tccgggtaat ttattggtca ttccgcctgt
     6601 ggacgtggct gccgccaaat tgccgtctaa atccaacgcc acggccccca cggtgcccat
     6661 tttctgtttt tcatccagtg gcgcaccgct atggtcgagg acggttgccc cttccttgcg
     6721 cgctgccagt agttgttcat aacgcaaagg cgtggagaaa atcgccggcg agacgcgctc
     6781 catgccacga gcaaacgcaa aattttctgc cccttcgcca atcatcatca catgcgggct
     6841 ttgctccatc accagccggg cggcaagaac cggattacgc agatgactaa cgcccgccac
     6901 cgcaccggct ttcagggtgt taccatccat cacacaggcg tccagttcat gggtttcatc
     6961 acgcgtaaag acagcgccaa ttccggcgtt aaacagtgga cactcttcca gcagacgcac
     7021 cgcttccgtc accacatcca gcgcactttc gcccgcttcc agcattttct gcccggtttc
     7081 aacaatggca gacaacgcct caatgtagcg taattcctgt tgcagactca tctgcgcgcg
     7141 gctaattgca cctgcgccac catgaattgc aatgactgct ttgcccatag tattcgtcca
     7201 ttaagggtgg ctcgccactt ttgctataaa tatcagaatt tttatgaatt actcattcaa
     7261 tttttgaata tagaaagatg gatatggcat gtaaaggcag gagccaacgc tacgcccgag
     7321 gtggcataca gtctgtggcg tttttctgac ataataggca aattcgattt tgcctccgca
     7381 ggagtgtttt catggaattt accaccggat tgatgtcgct cgacaccgcg cttaatgaga
     7441 tgctttctcg cgtcacccca ctgaccgccc aggaaacgct gccactggta cagtgttttg
     7501 gtcgtattct ggcgagcgat gtcgtttcgc cgcttgatgt tccggggttt gataactccg
     7561 caatggacgg ctacgcggtg cgtttagccg atattgcctc cgggcaaccg ctgcccgttg
     7621 ccggtaaatc ctttgccggt cagccgtatc atggtgaatg gcctgcgggc acctgcattc
     7681 gtattatgac cggtgcgccg gtgccggaag gctgcgaagc ggtggtgatg caggagcaga
     7741 ctgaacaaac ggacaatggc gtgcgtttta ctgctgaagt gcgtagcggg caaaatattc
     7801 gccgtcgcgg tgaagatatc tctgcaggtg cggttgtttt cccggcggga actcgcctga
     7861 ctaccgcaga gctgccagta attgcttcac tggggattgc cgaagttccg gtgattcgta
     7921 aagtgcgtgt agcgcttttt tctaccggtg atgaactcca gttgcccggt cagccgctgg
     7981 gtgacggcca aatctacgac accaaccgtc tcgccgtaca cctgatgtta gaacagctgg
     8041 gttgcgaggt aatcaactta gggattatcc gcgacgatcc ccatgccctg cgcgccgcat
     8101 ttattgaagc cgacagccag gcggatgtag tgatcagttc cggcggtgtt tcagtgggtg
     8161 aagcggatta caccaaaacg attcttgaag agctggggga gatcgccttc tggaagctgg
     8221 cgattaaacc aggtaaaccg ttcgcgttcg gtaaactcag caatagctgg ttctgcggcc
     8281 tgccgggcaa cccggtttca gcgacgctga ccttctatca actggtacag cctttgctgg
     8341 caaaactaag cggcaacacc gccagcggcc tgcccgcgcg ccagcgcgta cgcacagcgt
     8401 cccgcctgaa aaaaacgcca ggacgtcttg atttccagcg cggcgtgctg caacgcaacg
     8461 ccgacggtga acttgaagtg acgaccaccg gacatcaggg ttcacatata tttagctcct
     8521 ttagtctcgg caactgcttt atcgtgctgg aacgcgatcg cggcaatgtg gacgtgggcg
     8581 aatgggtgga agtagaaccg tttaacgcgt tgttcggagg cctgtaatgg cggaactcag
     8641 cgatcaggag atgctgcgct acaaccggca aatcattctg cgcggttttg attttgacgg
     8701 tcaggaggcg ctaaaagatt ctcgtgtgct gatcgtcggc ctcggtggac tcggctgcgc
     8761 agcttcgcag tatctggcaa gcgccggtgt cggcaacctg acgctgctcg acttcgacac
     8821 ggtttcgctc tcgaatctgc aacgccagac actgcacaat gatgccacgg tcgggcaacc
     8881 gaaggtggaa tccgcccgtg acgccctgac gcggatcaac ccacatatcg cgattacgcc
     8941 agtcaatgca ctgctagatg acgcagaact tgcagcattg attgctgaat acgatctggt
     9001 gctcgactgt acggataacg ttgcggtacg taatcaactg aacgcaggct gttttgccgc
     9061 gaaggtgccg ctggtttccg gcgcggcaat tcgtatggaa gggcaaatca cggtcttcac
     9121 ttatcaggac ggcgaaccgt gctatcgctg ccttagccgt ttgtttggcg agaacgcgtt
     9181 aacctgcgta gaagcaggcg tgatggcacc gttgatcggc gtaattggtt cgttgcaggc
     9241 aatggaagcg atcaaactgc tggcaggtta tggcaaaccg gccagcggga aaatcgtcat
     9301 gtacgatgcg atgacctgtc agtttcgcga aatgaaactg atgcgtaatc cggggtgtga
     9361 ggtgtgcggg cagtaattgc cacctccagg ccagataaga cgcggcactc cgccacgtca
     9421 tcagcaattt atgaggatgc agaacgtccg gttaaatcga cgttctgcca aacgctccca
     9481 gccagtcctg ttcaaacttc gccacagcgg catcaaccgc cggatagcta atcatctgtt
     9541 gtgccacatc cagtggcagc gtaattgatt cacatcctgc cagtaagcag tccagcgcct
     9601 gacgcgggtt tttgaaactc gctgccagca ctttcgcctg cggcgcatgc attttcaata
     9661 actggtgtaa gtcggtcaca gtctgaatgc cgctaccgcc ctgagcatca atacgattaa
     9721 cgtaaggcgc aacatattcc gcacctgcca gcgccgacag cagcccttgt gctgcgccat
     9781 ataccgcggt tcccagcgtt ggaatccctt ccgcttttaa catcttaata gctgccagcc
     9841 cctcggcggt caccggaact ttcaccacga tatccgcaat aatagaacgc aatttacgtg
     9901 cgtcattcac catcccttca gcagtggtag ccattacctg ggcaaacaga cgcccctgac
     9961 cgcccatcgc ttcatgaagt tgcggaagca caacatccag cggtttttta cccgcggcga
    10021 taatgcttgg gttagtggtc acacccgcca gcggaaaaat acgtgacagc gccttcactg
    10081 caacaacgtc tgaagtatcc agatacagtt ccataaccat cctcaaaatg tactttttgt
    10141 ctgaatgtag actatcacgg gaaaactctc tgaacagttg acgcacatca atccaacttt
    10201 cattcgaaag tattttaatg tttgaacgaa acagagaggc aactatgatt ttcaatattc
    10261 agcgctactc gacccatgat ggccccggta tccgcacggt cgtatttctt aaaggctgtt
    10321 cgctgggctg ccgctggtgt cagaacccgg aaagccgcgc ccgcacgcag gatctgctgt
    10381 atgacgcacg actgtgtctg gaaggctgcg agctgtgcgc taaggcagcg ccggaagtga
    10441 ttgagcgcgc attgaatggt ttgcttattc atcgggaaaa gttaaccccg gagcatctga
    10501 cggcgctaac cgactgctgc ccgacacagg cgttaaccgt gtgtggggaa gtgaaaagcg
    10561 ttgaggagat catggcgacc gttctgcgcg ataaaccgtt ttacgatcgc agcggcggcg
    10621 gcttaacgct ttcgggtggt gaacccttta tgcagccgga aatagcgacg gcgctactgc
    10681 aagcctgcca cgaggcaggc attcatactg cggtagaaac ctgtctgcat gtgccgtgga
    10741 aatatatcac cccttctctg ccctatatcg atctgtttct tgccgattta aaacacattg
    10801 ccgacgcgcc gtttaaacag tggaccgacg gtaacgccgc caaggtgctg gataacctga
    10861 aaaaactcgc cgcagcgggc aaaaaaatca ttatccgcgt gccgctgatt cagggcttta
    10921 atgccgacga aacctctgta aaagccatta ccgattttgc cgccgacgag ctgcacgttg
    10981 gcgaaattca ttttctgccc taccacacgc tgggcatcaa caaatatcac ttacttaatc
    11041 tgccctatga cgccccggaa aaaccgcttg atgcgccaga cctgctcgac tttgcccagc
    11101 agtatgcctg ccagaaaggg ttaaccgcga ccttacgagg ataacaacca tgaccacact
    11161 gaaactggac acgctcagcg accgcattaa agcgcacaaa aatgcgctgg tgcatattgt
    11221 gaaaccgccg gtctgtaccg agcgcgcgca gcactatacc gagatgtatc aacaacatct
    11281 cgataagccg atcccggtac gtcgcgcgct ggctctggcg catcacctgg cgaatcgcac
    11341 catctggatc aagcacgatg agttgatcat cggcaaccag gcaagcgagg ttcgcgctgc
    11401 gccgatcttc ccggaatata ccgtctcgtg gatcgaaaaa gagattgatg atctggcaga
    11461 tcgtcccggt gctggttttg cggtgagcga agagaacaaa cgcgttctgc atgaagtgtg
    11521 cccgtggtgg cgcggtcaga cggtacagga tcgctgctac ggcatgttta ccgatgagca
    11581 aaaaggtctg ctggcgaccg gaatcattaa agcggaaggc aatatgacct ccggcgatgc
    11641 gcacctggcg gtgaatttcc cgctgctgct ggaaaaaggt cttgatggtc tgcgcgagaa
    11701 agtagcggaa cgtcgctcgc gcatcaatct gacggtactg gaagatttgc acggcgaaca
    11761 gttcctgaaa gcgattgata tcgtgctggt ggcagtcagc gaacacattg aacgttttgc
    11821 agccctggcg cgtgaaatgg ccgcgaccga aacccgcgaa agccgtcgcg atgaactgct
    11881 ggcgatagca gaaaactgcg atcttatcgc ccaccagccg ccgcagactt tctggcaggc
    11941 gctgcaactg tgttacttca tccagttgat attgcagatc gaatctaacg gtcactcggt
    12001 atcgtttggt cgtatggacc agtatctcta cccgtactat cgccgagacg ttgaactcaa
    12061 ccagacactg gatcgcgaac acgccatcga attgctgcat agctgctggc tgaaactgct
    12121 ggaagtgaac aagatccgct ccggctcaca ctcaaaagcc tctgcgggaa gtcctctgta
    12181 tcagaacgtc accattggcg gacaaactct ggttgatggt caaccaatgg acgcggtgaa
    12241 tccactctct tacgcgatcc tcgaatcctg cggtcgcctg cgttccactc agcctaacct
    12301 cagcgtgcgt taccatgcag gaatgagtaa tgatttcctc gacgcctgcg tacaggtgat
    12361 ccgttgcggc ttcgggatgc cggcgttcaa caacgacgaa atcgtgatcc cggaatttat
    12421 taaactcggt attgaaccgc aggacgctta cgactacgca gcgattggtt gtatcgaaac
    12481 cgccgtcggt ggcaaatggg gctatcgctg taccggcatg agctttatca acttcgcccg
    12541 cgtgatgctg gcggcgctgg aaggcgggcg tgatgccaca agcggcaacg tgttcctgcc
    12601 acaagaaaaa gcgttgtcgg caggcaactt taacaatttc gatgaagtaa tggacgcgtg
    12661 ggatacgcaa atccgttatt acacgcgtaa atcaatcgaa atcgaatatg tcgtcgacac
    12721 catgctggaa gagaacgtgc acgatattct ctgctcggcg ctggtggatg actgtattga
    12781 gcgagcgaaa agtatcaagc aaggcggcgc gaaatatgac tgggtttctg gcctgcaggt
    12841 tggcattgcc aacctcggca acagcctggc ggcagtgaag aaactggtat ttgaacaggg
    12901 cgcgattggt cagcaacagc tggctgccgc actggcggat gacttcgatg gcctgactca
    12961 tgagcaactg cgtcagcggc tgattaacgg tgcgccgaag tacggcaacg acgatgatac
    13021 tgtcgatacg ctgctggctc gcgcttatca gacctatatc gacgaactga aacagtacca
    13081 taatccgcgc tacggtcgtg gtccggttgg cggcaactat tacgcgggta cgtcatctat
    13141 ctccgctaac gtaccgtttg gtgcgcaaac tatggcaacg ccggacgggc gtaaagccca
    13201 caccccgctg gcagaaggcg ctagtccggc ctctggtact gaccatctcg gccctactgc
    13261 ggtcattggc tcggtgggta aactgcctac ggcagcgatt ctcggcggcg tgttgctcaa
    13321 ccagaaactg aatccggcaa cgctggagaa cgaatctgac aagcagaagc tgatgatcct
    13381 gctgcgcacc ttcttcgagg tgcataaagg ctggcatatt cagtacaaca tcgtttcccg
    13441 cgaaacgctg ctggaggcga aaaaacatcc ggatcagtac cgcgatctgg tagtgcgtgt
    13501 cgcgggttat tccgccttct tcaccgcgct ctctccagac gctcaggacg atatcatcgc
    13561 tcgtactgaa catatgctgt aatctcttcg gcccggctgc ggcgtcgccg ggctaaatca
    13621 ttcacatcat caatttcatc cttactttca ttcgaaatat aatttgtgct ctgcgtcaca
    13681 ttgttattac actgttacgg tttcattttc gagccaggag cacacctatg agcgtaaaag
    13741 ttatcgtcac agacatggac ggtacttttc ttaacgacgc caaaacgtac aaccaaccac
    13801 gttttatggc gcaatatcag gaactgaaaa agcgcggcat tgagttcgtt gtcgccagcg
    13861 gtaatcagta ttaccagctt atttcgttct ttcctgagct aaaggatgag atctcttttg
    13921 tcgcggaaaa cggcgcactg gtttacgaac atggcaagca actgttccac ggcgaactga
    13981 cccgacatga atcgcggatt gttattggcg agttgctaaa agataagcaa ctcaattttg
    14041 tcgcctgcgg tctgcaaagt gcatatgtca gcgaaaatgc ccccgaagca tttgtcgcac
    14101 tgatggcaaa acactaccat cgcctgaaag ctgtaaaaga ttatcaggag attgacgacg
    14161 tactgttcaa gttttcgctc aacctgccag atgaacaaat cccgttagtg atcgacaaac
    14221 tgcacatagc gttcgatggc attatgaaac ccgtcaccag tggttttggc tttatcgacc
    14281 tgattattcc cggtctacat aaagcaaacg gtatttcgcg gttactgaaa cgctgggatc
    14341 tgtcaccgca aaatgtggta gcgattggcg acagcggtaa cgatgcggag atgctgaaaa
    14401 tggcgcgtta ttcctttgcg atgggcaatg ctgcggaaaa cattaaacaa atcgcccgtt
    14461 acgctaccga tgataataat catgaaggcg cgctgaatgt gattcaggcg gtgctggata
    14521 acacatcccc ttttaacagc tgacctctct accggagccg tgcgctccgg ttttcccttc
    14581 tttttacatc ctgtacatca catcaaatca ttaaacttgc attaacttat ttataactta
    14641 tattgtcaca tgcaaatcat aatggtttcg aatgaaagtt aatgagaggt ggttatggct
    14701 tctactttta ccagcgacac attgccagcc gatcacaaag cagctatccg tcagatgaag
    14761 cacgcgctgc gggcgcagct tggcgacgtc cagcagatct ttaatcagct aagcgatgac
    14821 attgccacgc gagtggctga aatcaacgca ctcaaagcac agggcgatgc cgtctggccg
    14881 gtgctgtctt atgccgatat caaagcaggt catgttactg cagagcagcg cgaacagatt
    14941 aaacgtcgcg gttgtgcggt gataaaaggc catttctccc gcgaacaagc gctaggctgg
    15001 gatcagtcga tgctggacta tctggaccgc aaccgctttg acgaggtcta caaaggcccc
    15061 ggcgataatt tcttcgggac gctcagcact tcacgtcccg agatttaccc catctactgg
    15121 tcgcagacgc aaatgcaggc ccgccagagt gaagaaatgg cgaatgcgca gtcgtttctc
    15181 aatcgtctgt ggacatttga aagtgatgga aagcaatggt ttaacccgga tgtgagcgtc
    15241 atctaccctg accgtatccg ccgccgtccg cccggaacga cctccaaagg tcttggagcg
    15301 cataccgact ccggggcgct ggaacgctgg ctgcttccag cgtatcagcg cgttttcgcc
    15361 aacgtcttta atggcaatct ggcgcaatat gatccctggc atgcggcaca tcgtacggaa
    15421 gttgaagagt acacggtgga caacaccacc aaatgttccg tgtttcggac attccagggc
    15481 tggacagcgc tctctgatat gctgcctggt caggggttgc tgcacgttgt gcccattcct
    15541 gaagccatgg cgtacgtact gttacgtccg ctgcttgatg atgtgccgga ggatgaactg
    15601 tgcggcgtag cgcccggaag agtgttgccg gtatcagagc aatggcatcc actgttaatt
    15661 gaggcgttaa ccagcattcc aaaactcgag gccggagact ccgtctggtg gcactgcgac
    15721 gtcatccatt ccgttgcccc cgttgaaaat caacagggtt ggggcaacgt gatgtacatt
    15781 cctgcggcac cgatgtgcga gaaaaatctt gcctacgcgc acaaggtgaa ggccgcactg
    15841 gaaaaaggcg catcgccggg cgacttcccg cacgaggact atgaaacaaa ctgggaagga
    15901 cgctttacgc ttgccgacct caacattcac ggtaagcgag cgttgggcat ggatgtttga
    15961 gagttaatgg aagtgaattg atgtgggatt aagaaaatcg attcgacgtt actcagattg
    16021 ctgacaagtg cgctttctta atgcctgatg cggcgttaac gccttatctg gcctacaaag
    16081 tcctgcagat tcaatatatt acaggtactg cgtaggcctg ataagcgtag cgcatcaggc
    16141 tattttgcat ttgtcatcag ccgccgtcac cccgacagta tcggggtgac ggataatcac
    16201 ttactcgatc cctttactac gcaggtaatc ttcgtaatta ccgctaaagt cgatcacgcg
    16261 ttccggggtg atttccagaa tgcgggtcgc cagggagctt acgaactcac ggtcgtgtga
    16321 aacaaagatc agcgtgccct gatacagttc cagcgccatg ttcagtgact cgatggattc
    16381 catatccagg tggttggtcg gttcgtccat gatcagaatg ttcggcttct gcatcattaa
    16441 cttaccaaac agcatccgcc ctttttcccc accggaaagc actttagctg gctttttgat
    16501 gtcgtcctgg ctgaacagca aacgaccgag aatgctgcgt accgcctgct cgtcatcgcc
    16561 ttcctgcttc cactgactca tccattcgaa cacggtcaga tcattttcaa actcatattc
    16621 gtggtcctgg gcgtagtaac caatgcgcgc gttttcagac cattttacgg tgccgctgtc
    16681 cggttgcaga tcgcccacca gcgttttcat cagcgttgat ttaccgacgc cgttggtacc
    16741 cagtaccgca agtttttcac cgacctccag cagcaggttg agatttttaa acagtggacc
    16801 gttatcaaac cctttggtca gaccttccac ttccagcgcg ttacggaaca gtttcttatc
    16861 ctgttcaaaa cggatgaacg ggttctgacg gctggaggct ttcacctctt ccagtttgat
    16921 tttatcaatc tggcgcgcgc gcgaagttgc ctggcgagat ttcgaggcgt tggcgctaaa
    16981 gcggctaacg aaagattgca actcagcaat ctgcgctttc ttcttggcgt tatcggccag
    17041 cagacgttca cgcgcctggg tcgccgccgt catgtactca tcgtagttac ccggataaac
    17101 gcgcagctcg ccgtaatcca gatccgccat gtgggtacag accatgttaa ggaagtgacg
    17161 gtcgtgcgag atgatgatca tggtgctgtc acgctcgttc agcacctgtt ccagccagcg
    17221 aatggtgtcg atgtcgaggt tgttggtcgg ttcgtcgagc aggagaatat ccggatcagc
    17281 aaacagcgcc tgcgccagaa gcacacgcag cttccagcca ggagcaactt cactcatcgg
    17341 gccgtagtgc tgttccactg gaattcccac gccaagcaac aattcaccgg cgcgagcttc
    17401 cgcagagtaa ccgtccattt cgccgtattt aacttccaga tcggccactt tatagccgtc
    17461 ttcctcactc atttccggca aagcatagat gcggtcacgc tcctgcttca cttcccacaa
    17521 ctctttatgc cccatgatca ccgtatccag cacagtgaac tcttcaaagg caaactgatc
    17581 ctgacgcagc ttaccaatgc gctcgttggg atcgagggaa acgttaccca aagtcggctc
    17641 taagtcgccg ccgaggatct tcataaaggt ggatttacca ctaccgttcg cgccaatcag
    17701 gccgtaacgg ttgccgccgc caaatttgac ggaaatgttt tcaaacaacg gcttactgcc
    17761 gaactgcatg gtgacgttac tggaaactaa cacaggcgta tcctgaaaag agatatgaca
    17821 aaccgcgcat tatgccataa gtctgaaaga agatcctgct cctcgcttaa ggtctacact
    17881 caagccaaag gcaaaaaatg tgatttcgta cacatctgat ttcactgtga gctggaatga
    17941 acttataatg cgcttccaat actctaatat tctcaaccca atggcctgcc aggcacaaaa
    18001 tctcgcttaa catgaatatg aaattgaaaa cattattcgc agcggccttc gctgttgtcg
    18061 gcttttgcag taccgcctct gcggtaactt atcctctgcc aaccgacggg agtcgcctgg
    18121 ttggtcagaa tcaggtgatc accattcctg aaggtaacac tcagccgctg gagtattttg
    18181 ccgcggagta ccagatgggg ctttccaata tgatggaagc gaacccgggt gtggatacct
    18241 tcctgccgaa aggcggtact gtactgaaca ttccgcagca gctgatcctg ccggataccg
    18301 ttcatgaagg catcgtcatt aacagtgcag agatgcgtct gtattactat ccgaaaggga
    18361 ccaacaccgt tatcgtgctg ccgatcggca ttggtcagtt aggcaaagat acgcctatca
    18421 actggaccac caaagttgag cgtaagaaag caggcccgac ctggacgccg accgcaaaaa
    18481 tgcacgcaga gtaccgcgct gcgggcgaac cgcttccggc tgtcgttccg gcaggtccgg
    18541 ataacccgat gggtctgtat gcactctaca tcggtcgcct gtatgctatc catggcacca
    18601 acgccaactt cggtatcggc ctgcgtgtaa gtcatggttg tgtgcgtctg cgtaacgaag
    18661 acatcaaatt cctgttcgag aaagtaccgg tcggtacccg cgtacagttt attgatgagc
    18721 cggtaaaagc gaccaccgag ccagacggca gccgttatat tgaagtccat aacccgctgt
    18781 ctaccaccga agcccagttt gaaggtcagg aaattgtgcc aattaccctg acgaagagcg
    18841 tgcagacagt gaccggtcag ccagatgttg accaggttgt tcttgatgaa gcgattaaaa
    18901 accgctccgg gatgccggtt cgtctgaatt aatcttcaaa acttaaagca aaaggcggac
    18961 tcataatccg ccttttttat ttgccagacc ctagttggcc gggagtataa ctaacaaaac
    19021 atatcccacc aacgccgccc acaacagcat cggtattgaa tagagatgga aacgccacca
    19081 gatgcggcga tcgttcgcca tacgtagcgc aatcaaactt gccagcgatc cgggtaataa
    19141 cccaaagcca cctacgttta ccgcccatgc cagtaataaa gacggcggca catagttcag
    19201 caacaatatg gtactcggca cgttactgat cacctgcgat aaaccgattg ccgttaacca
    19261 taacccgggt tcagatagat gactcacgtt acccaacacg ccttgcaacg ctggaagctg
    19321 ggtcagtaaa tggacgtcga taaacatcgc cataaacacc agcagcagcg tccagtccac
    19381 actgagcacc acgcgacgtg ccagcagcgc aaagcctgcc gccacaatca ccagtcccca
    19441 cagctcttgt ttgaactcca gcgccgttag aaagacgata tacagcccca gacaactcca
    19501 caccagccgc ggtttccact ccggtgtttg cacccccgta tggtattgca gcacctttcc
    19561 agggaaacaa caccagcaca ggagcaggag cgtcagcatc attgcgccag ccagcggtgc
    19621 catttgggca ataaatccgg caaacgaaag accagaacgt ccccagataa gaatattttg
    19681 cgggttgcca attggcgtca gtagcgaacc agcattgact gccagcgcct caaaaataat
    19741 cagacgatta accgggatct cacacagtct ttttagcgtg atagtcagcg gaacaacaat
    19801 aaacagcgcg acatcgttgg tcagaaaggt agaaagcagc gccgccgcca gcaccataaa
    19861 catcgccagc cgacgctccg tagcaaagcg gcgcaccatt ttgcgcccca gcacatcaaa
    19921 ataaccgctt aactccacac ctttggtcag cagcatcagg ccgcttaagg tgatgatggt
    19981 gtgccagtcg atagcagcag gccaggattt cggtgcaaag ggcacgaaaa agcttaatcc
    20041 gataccaaca agaattaata actgaaaaaa acgatcgcct tgcagcgtgc gtaaaaaagg
    20101 caggctcatt tggcaccgtg tttctgggta aacaaacgaa aagcgtcgag cgtctcttca
    20161 ctaacatggt gctccatgcc ttccgcgtcg cgacgggcga tttccggact gacgcccaac
    20221 accaacaaga aattttcgac tatctgatgt cgctcgcggc tttcctgcgc cagcttctct
    20281 ccttctgccg ttaaaaacac gccacgccag gggatcattt caatcagccc catggttgcc
    20341 agccgcttaa gcattttagc caccgtcggt tgcgaaactc ccagacgagc agccatgtcc
    20401 acctgacgag cttcccccac ttccctgatc aagtcagaaa tcagctcaac gtaatcatca
    20461 ataagctcgc gccgatgcgc ctctctgacc tggcggaacc cttcaacgtg ctcttccacg
    20521 ttcactaact gcgtcacttt ttttgctgtt ggcgtacctg cgcgacgact cattgtgctt
    20581 cctcattatg gtgacgcata catagcgttc tcatttcgtg cgcacattgt aaaccagagt
    20641 tgcgaaggta caaaaaatta acgttttagc aatagctata taatatagcc tgtgctatat
    20701 ctgtatgtaa tgcaatcatc cctcaaggat cgacgggatt agcaagtcag gaggtcttat
    20761 gaatgagttc aagaggtgta tgcgcgtgtt tagtcattct ccctttaaag tacggttaat
    20821 actgctctct atgttgtgcg atatggtcaa caacaaaccg cagcaagata aaccttccga
    20881 taaatagcgg cgtcgcggta cgccgcttca ctcctgcttt catgcaggca taacgcgttt
    20941 tggcctgaaa aaccccactt tttgtcggat ttgcaatccc cttcgcaaaa gatttgttcg
    21001 tcagtagttg acctgaacgg cggctcgctc tatcttcttg cagccctgcg tatattgcgg
    21061 ctcgcggatg cggacccctt tccactcttc acgcactctt gcaggtattg acccttgacg
    21121 ccagggtaag cacatggcgt ttgttacgat agtggcatat gaatttaacc ctcaaagaat
    21181 cgcttgttac ccgtagccgg gtatttagcc cgtggactgc gttctacttt ttacagtcgc
    21241 tattaattaa cctcggctta ggttacccct tcagtttgct ctacaccgct gcgtttacgg
    21301 ctattttgct tttgctatgg cgaacattgc ctcgcgtaca aaaagttctg gtcggtgtca
    21361 gttcgctggt ggcggcttgt tatttccctt ttgctcaggc ctacggcgcg cctaatttca
    21421 atacattgct ggcattgcac tccaccaata tggaagagtc gaccgaaatc ctgacgattt
    21481 ttccgtggta cagctacctg gtcggcttat ttatttttgc gctcggcgta atagcaatca
    21541 ggcgaaaaaa agagaatgaa aaagcgcgct ggaatacctt cgacagcctg tgtctggtat
    21601 tcagtgtggc gacatttttt gttgctcccg tgcaaaacct ggcctggggt ggcgtattta
    21661 aactgaaaga tactggctat ccggtatttc gttttgctaa ggatgtcatc gtcaataata
    21721 acgaggtgat tgaagagcaa gaacggatgg caaaactttc cggaatgaaa gatacctgga
    21781 cggtcactgc cgttaagccg aagtatcaga cctatgtggt ggtgatcggt gaaagcgcgc
    21841 gtcgcgatgc cctcggtgcc tttggcggtc actgggacaa taccccgttt gccagcagcg
    21901 ttaacggttt gatatttgct gactacattg ccgccagtgg ctccacgcag aaatcgcttg
    21961 gcttaacgct caatcgcgta gtcgatggca aaccacagtt tcaggataac tttgtcaccc
    22021 tggcaaatcg cgcgggcttc cagacctggt ggttttccaa ccagggccaa atcggcgaat
    22081 acgatactgc tatcgccagc atcgccaaac gtgcagatga agtgtacttc ctgaaagaag
    22141 gtaattttga agcagataaa aacacgaaag acgaagcgtt actggatatg accgctcaag
    22201 tgctggcgca agagcactcg caaccgcagc tgattgttct gcatctgatg ggctcgcatc
    22261 cgcaggcctg cgacaggaca caaggcaaat acgaaacctt tgtgcaatcg aaagaaacgt
    22321 cgtgctatct ctataccatg acgcaaacgg acgatttact gcgcaagctg tacgatcagt
    22381 tacgcaacag cggcagcagc ttctctctgg tttacttttc tgaccacggt ctggccttta
    22441 aagagcgcgg caaagacgtg caataccttg cccatgatga taaatatcag caaaatttcc
    22501 aggtgccttt tatggtcatt tccagcgacg ataaagcgca tcgggtgatt aaagcccgcc
    22561 gctcagccaa tgacttctta ggctttttct cgcagtggac gggaattaaa gcgaaggaaa
    22621 taaatatcaa atacccgttt atatctgaga agaaagccgg gccgatatac atcaccaact
    22681 tccagttaca gaaggtggat tacaaccatc tcggaaccga tattttcgac ccgaaacctt
    22741 aaaaacaaaa atccgccccg agaggcggat tttttatatc accaaagtga ttagaagcgg
    22801 taaccaacac cggcaatcca ggtgcctacg tcaacgctac gaatacggct ctgctcgtaa
    22861 gagaagtcca gagcaacgtt ttccatcggg ttgaactgca gaccagcacc gtaggagaaa
    22921 ccgtagtcgc tggtgtcgtg tttgtaggtc gggtattcag tggtctggaa tttaccataa
    22981 cccacaccca ctacaccgta gatgcttgcc cagtcgttaa tgcggtaagc cggaccagca
    23041 gtgatgccgt agtactggtt tttgttgtag tcaccagagc ttgcagtacg acttttctcg
    23101 gtgtaagtga aagaaccgat cacacccagc gggctgttgt cttcttcata gcggtatttc
    23161 aggttgaaac cgcccatttt gttcatttgg ccctgagcgt cgctctgtgc gtaaccgcca
    23221 gttacggtag aagtcgcagc tacggaagta cctgcggtga aagccagaac tgcggccagt
    23281 gctgaaagac atgcaatttt tttcataacc acctcaaatg tgattcaaat aagtcctaag
    23341 ttttaaatat atcaaaaatt aatgggaaac tcttcgcgat ttgtgatgtc taacgggcca
    23401 tttcatgtaa cagaacgttt ccatacaccg ctatccatct aaatttaaat cactttttca
    23461 gagaactgcg taagtattac gcatgttttc cctgtcattc atccagatta ttcctaatca
    23521 ccagactaat gattccatca atcctggcgc attttagtca aaacggggga aaattttttc
    23581 aacaaatgct caaccagcat tgggtatatc cagtacactc cacgctttac ttaagtctag
    23641 atatttgtgg gagaaaggat gcctggttca ttacgtaaaa tgccggtctg gttaccaata
    23701 gtcatattgc tcgttgccat ggcgtctatt cagggtggag cctcgttagc taagtcactt
    23761 tttcctctgg tgggcgcacc gggtgtcact gcgctgcgtc tggcattagg aacgctgatc
    23821 ctcatcgcgt tctttaagcc atggcgactg cgctttgcca aagagcaacg gttaccgctg
    23881 ttgttttacg gcgtttcgct gggtgggatg aattatcttt tttatctttc tattcagaca
    23941 gtaccgctgg gtattgcggt ggcgctggag ttcaccggac cactggcggt ggcgctgttc
    24001 tcttctcgtc gcccggtaga tttcgtctgg gttgtgctgg cggttcttgg tctgtggttc
    24061 ctgctaccgc tggggcaaga cgtttcccat gtcgatttaa ccggctgtgc gctggcactg
    24121 ggggccgggg cttgttgggc tatttacatt ttaagtgggc aacgcgcagg agcggaacat
    24181 ggccctgcga cggtggcaat tggttcgttg attgcagcgt taattttcgt gccaattgga
    24241 gcgcttcagg ctggtgaagc actctggcac tggtcggtta ttccattggg tctggctgtc
    24301 gctattctct cgaccgctct gccttattcg ctggaaatga ttgccctcac ccgtttgcca
    24361 acacggacat ttggtacgct gatgagcatg gaaccggcgc tggctgccgt ttccgggatg
    24421 attttcctcg gagaaacact gacacccata cagctgctgg cgctcggcgc tatcatcgcc
    24481 gcttcaatgg ggtctacgct gacagtacgc aaagagagca aaataaaaga attagacatt
    24541 aattaaattt acatttctgc atggttatgc ataaccatgc agaatttctc gctacttttc
    24601 ctctacaccg tctttatata tcgaattatg caaaagcata tttattccga aaattcctgg
    24661 cgagcagata aataagaatt gttcttatca atatatctaa ctcattgaat ctttattagt
    24721 tttgtttttc acgcttgtta ccactattag tgtgatagga acagccagaa tagcggaaca
    24781 catagccggt gctatactta gtctcgttaa ttactgggac ataacatcaa gaggatatga
    24841 aattatgagt accgctaaat tagttaaatc aaaagcgacc aatctgcttt atacccgcaa
    24901 cgatgtctcc gacagcgaga aaaaagcaac agtagagttg ctgaatcgcc aggttatcca
    24961 gtttattgat ctttctttga ttaccaaaca agcgcactgg aacatgcgcg gcgctaactt
    25021 cattgccgta catgaaatgc tggatggctt ccgcaccgca ctgatcgatc atctggatac
    25081 catggcagaa cgtgcagtgc agctgggcgg tgtagctctg gggaccactc aagttatcaa
    25141 cagcaaaacc ccgctgaaaa gttacccgct ggacatccac aacgttcagg atcacctgaa
    25201 agaactggct gaccgttacg caatcgtcgc taatgacgta cgcaaagcga ttggcgaagc
    25261 gaaagatgac gacaccgcag atatcctgac cgccgcgtct cgcgacctgg ataaattcct
    25321 gtggtttatc gagtctaaca tcgaataaat ccatcgctga tggtgcagaa ctttagtacc
    25381 cgataaaagc ggcttcctga caggaggccg ttttgttttg cagcccacct caacgcactt
    25441 atttagtgca tccatctgct atctccagct gattaagtaa attttttgta tccacatcat
    25501 cacacaatcg ttacataaag attgtttttt catcaggttt tacgctaaat aatcactgtg
    25561 ttgagtgcac aattttagcg caccagattg gtgccccaga atggtgcatc ttcagggtat
    25621 tgccctataa atcgtgcatc acgtttttgc cgcatctcta aaaatcaatg agttgcaaaa
    25681 ctggcacgat tttttcatat atgtgaatgt cacgcagggg atcgtcccgt ggatagaaaa
    25741 aaggaaatgc tatgaagtct gtattaaaag tttcactggc tgcactgacc ctggtttttg
    25801 cggtttcttc tcatgccgcg gataaaaaat tagttgtcgc gacggatacc gccttcgttc
    25861 cgtttgaatt taaacagggc gataaatatg tgggctttga cgttgatctg tgggctgcca
    25921 tcgctaaaga gctgaagctg gattacgaac tgaagccgat ggatttcagt gggatcattc
    25981 cggcactgca aaccaaaaac gtcgatctgg cgctggcggg cattaccatc accgacgagc
    26041 gtaaaaaagc gatcgatttc tctgacggct actacaaaag cggcctgtta gtgatggtga
    26101 aagctaacaa taacgatgtg aaaagcgtga aagatctcga cgggaaagtg gttgctgtga
    26161 agagcggtac tggctccgtt gattacgcga aagcaaacat caaaactaaa gatctgcgtc
    26221 agttcccgaa catcgataac gcctatatgg aactgggcac caaccgcgca gacgccgttc
    26281 tgcacgatac gccaaacatt ctgtacttca tcaaaaccgc cggtaacggt cagttcaaag
    26341 cggtaggtga ctctctggaa gcgcagcaat acggtattgc gttcccgaaa ggtagcgacg
    26401 agctgcgtga caaagtcaac ggcgcgttga aaaccctgcg cgagaacgga acttacaacg
    26461 aaatctacaa aaaatggttc ggtactgaac cgaaataata acgctacacc tgtaaagcgc
    26521 actggcagtt ccctctcccc tctggggaga ggattagggt gaggggcgca aacccgctcc
    26581 ggggccatta attaccctga atttgattat ttacaccacg gtaacaggaa caacatatgc
    26641 agtttgactg gagtgccatc tggcctgcca ttccgcttct gattgaaggt gccaaaatga
    26701 ccctgtggat ttcggtcctc ggtctggctg gcggtctggt aatcggattg ctggcaggtt
    26761 ttgcacgcac cttcggtggt tggatagcca accacgtcgc gctggtcttt attgaagtga
    26821 tccgcggcac acctatcgtc gtccaggtga tgtttatcta tttcgccctg ccgatggcgt
    26881 ttaacgactt acgcatcgac ccatttactg cggcggtggt caccatcatg atcaactccg
    26941 gcgcgtacat tgcggaaatc acgcgtggtg cggtgctgtc tattcacaaa ggttttcgtg
    27001 aagcaggact ggcgctcggt ctttcacgtt gggaaaccat tcgctacgtc attttaccgc
    27061 tggcactgcg tcgtatgctg ccgccgctgg gtaaccagtg gatcatcagc attaaagaca
    27121 cctcgctgtt tattgttatc ggcgtggcgg aactgacccg tcaggggcaa gaaattattg
    27181 ccggtaactt ccgcgccctt gagatctgga gcgccgtggc ggtgttctat ctgattatta
    27241 ccctggtgct gagctttatt ctgcgtcgtc tggaaagaag gatgaaaatc ctgtgattga
    27301 atttaaaaac gtctccaagc actttggccc aacccaggtg ctgcacaata tcgatttgaa
    27361 cattgcccag ggcgaagtcg tggtgattat cgggccgtcc ggttccggta aatcgaccct
    27421 gctgcgctgc atcaacaaac tggaagaaat cacctccggc gatctgattg tcgatggcct
    27481 gaaggttaac gatccgaaag ttgacgagcg cctgattcgc caggaagcag gtatggtgtt
    27541 ccagcagttt tacctcttcc cgcatctgac ggcgctggaa aacgtcatgt ttggtccgct
    27601 acgcgtgcgt ggcgcgaaca aagaagaggc ggaaaaactg gcgcgtgagc tgctggcgaa
    27661 agtcggtctg gcagaacgtg cacatcacta cccttccgaa ctttctggtg gtcaacagca
    27721 gcgtgtggcg attgcccgcg cgctggcggt gaagccgaaa atgatgctgt ttgatgaacc
    27781 gacttccgct cttgacccgg aactgcgcca tgaagtgctg aaggttatgc aggatctggc
    27841 tgaagaaggg atgacgatgg tgatcgtgac ccacgaaatc ggttttgccg agaaagtagc
    27901 ttcgcggctg atctttatcg acaaaggccg gattgcggaa gatggcaatc cgcaggtgtt
    27961 gatcaagaac ccgccgagcc agcgcttgca ggaatttttg cagcacgtct cttaataaga
    28021 cacattgcct gatgcgctac gcttatcagg cctacaggat atctggcaac ttattaaaat
    28081 tgcatgaact tgtaggacgg ataaggcgtt cacgccgcat ccggcaaaaa agcccgcacg
    28141 ttgtcagcaa tctgcttaat atcccttcct ccctttcacc cgaaagggag gcacaccaga
    28201 ttcctctcat ttaaaatcgc ccc
//
LOCUS       DAJTMC010000036        27868 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.036, whole genome shotgun sequence.
ACCESSION   DAJTMC010000036 DAJTMC010000000
VERSION     DAJTMC010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27868
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.036"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            <1..527
                     /locus_tag="OO130_001395"
     CDS             <1..527
                     /locus_tag="OO130_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HCR5364863.1"
                     /translation="KYKIMANHHGIIFDEQRKRYIIGSITVSIDEMTNALDPVELAEI
                     INKKHHAVINGKKFKNSRAISCRSFNRYFSVSTDYRPKLEALLACSQMLGINQVVAHN
                     GNDGRECIGETGTVLGLNARDSKHAGRMFSMHNCQINPGAGPEITTPWKSYQHEKNRN
                     GLMPLIRRRTMLQL"
     gene            893..1960
                     /locus_tag="OO130_001396"
     CDS             893..1960
                     /locus_tag="OO130_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR5364864.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHMRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            1957..4692
                     /gene="rbbA"
                     /locus_tag="OO130_001397"
     CDS             1957..4692
                     /gene="rbbA"
                     /locus_tag="OO130_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HCR5364865.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVMVLGGDMRDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSTLSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDMWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            4692..5816
                     /locus_tag="OO130_001398"
     CDS             4692..5816
                     /locus_tag="OO130_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5364866.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            5889..6163
                     /locus_tag="OO130_001399"
                     /pseudo
     CDS             5889..6163
                     /locus_tag="OO130_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
     gene            6160..6519
                     /locus_tag="OO130_001400"
     CDS             6160..6519
                     /locus_tag="OO130_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HCR5364867.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLLEYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(6639..7040)
                     /gene="nikR"
                     /locus_tag="OO130_001401"
     CDS             complement(6639..7040)
                     /gene="nikR"
                     /locus_tag="OO130_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HCR5364868.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(7046..7852)
                     /gene="nikE"
                     /locus_tag="OO130_001402"
     CDS             complement(7046..7852)
                     /gene="nikE"
                     /locus_tag="OO130_001402"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HCR5364869.1"
                     /translation="MTLLNVSGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWRGESLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDETVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQAVGDKLTFSSDAGRVLQNAVLPAFPVRRRATEKV"
     gene            complement(7849..8613)
                     /gene="nikD"
                     /locus_tag="OO130_001403"
     CDS             complement(7849..8613)
                     /gene="nikD"
                     /locus_tag="OO130_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HCR5364870.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKAVYPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            complement(8613..9446)
                     /gene="nikC"
                     /locus_tag="OO130_001404"
     CDS             complement(8613..9446)
                     /gene="nikC"
                     /locus_tag="OO130_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HCR5364871.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSH
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVSGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(9443..10387)
                     /gene="nikB"
                     /locus_tag="OO130_001405"
     CDS             complement(9443..10387)
                     /gene="nikB"
                     /locus_tag="OO130_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HCR5364872.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQFFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(10387..11961)
                     /gene="nikA"
                     /locus_tag="OO130_001406"
     CDS             complement(10387..11961)
                     /gene="nikA"
                     /locus_tag="OO130_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HCR5364873.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKLSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKTPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKALLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(12072..12659)
                     /gene="acpT"
                     /locus_tag="OO130_001407"
     CDS             complement(12072..12659)
                     /gene="acpT"
                     /locus_tag="OO130_001407"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HCR5364874.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(12714..13763)
                     /locus_tag="OO130_001408"
     CDS             complement(12714..13763)
                     /locus_tag="OO130_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR5364875.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVTITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMIGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLALM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYACILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            13895..15118
                     /locus_tag="OO130_001409"
     CDS             13895..15118
                     /locus_tag="OO130_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5364876.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALALASAGFGVIATFITLFYDAKSWDGAAFALTLFSCAFVGTRLLFPNG
                     INRIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKA
                     VPQQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRL
                     KKRPPEHVPEAASSS"
     gene            complement(15122..15679)
                     /gene="dcrB"
                     /locus_tag="OO130_001410"
     CDS             complement(15122..15679)
                     /gene="dcrB"
                     /locus_tag="OO130_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HCR5364877.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAENNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(15752..16417)
                     /locus_tag="OO130_001411"
     CDS             complement(15752..16417)
                     /locus_tag="OO130_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HCR5364878.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSILGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            16638..16883
                     /gene="tusA"
                     /locus_tag="OO130_001412"
     CDS             16638..16883
                     /gene="tusA"
                     /locus_tag="OO130_001412"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HCR5364879.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(16985..19183)
                     /gene="zntA"
                     /locus_tag="OO130_001413"
     CDS             complement(16985..19183)
                     /gene="zntA"
                     /locus_tag="OO130_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HCR5364880.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIAHQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQSATHPLAQAIVREAQVAALAIPAAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEYKVKAVTELNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(19257..19883)
                     /locus_tag="OO130_001414"
     CDS             complement(19257..19883)
                     /locus_tag="OO130_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000964717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HCR5364881.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYF"
     gene            20024..20383
                     /locus_tag="OO130_001415"
     CDS             20024..20383
                     /locus_tag="OO130_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HCR5364882.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(20386..20655)
                     /locus_tag="OO130_001416"
     CDS             complement(20386..20655)
                     /locus_tag="OO130_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HCR5364883.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(20645..21241)
                     /gene="rsmD"
                     /locus_tag="OO130_001417"
     CDS             complement(20645..21241)
                     /gene="rsmD"
                     /locus_tag="OO130_001417"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HCR5364884.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            21391..22884
                     /gene="ftsY"
                     /locus_tag="OO130_001418"
     CDS             21391..22884
                     /gene="ftsY"
                     /locus_tag="OO130_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HCR5364885.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     AKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPVEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISLF
                     RGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEMG
                     EILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFELQGKSVMLAAGDTFRA
                     AAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKSH
                     LMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLDG
                     TAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            22887..23555
                     /gene="ftsE"
                     /locus_tag="OO130_001419"
     CDS             22887..23555
                     /gene="ftsE"
                     /locus_tag="OO130_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HCR5364886.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            23548..24606
                     /gene="ftsX"
                     /locus_tag="OO130_001420"
     CDS             23548..24606
                     /gene="ftsX"
                     /locus_tag="OO130_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HCR5364887.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTNFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            24851..25705
                     /gene="rpoH"
                     /locus_tag="OO130_001421"
     CDS             24851..25705
                     /gene="rpoH"
                     /locus_tag="OO130_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HCR5364888.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            25976..27079
                     /gene="livJ"
                     /locus_tag="OO130_001422"
     CDS             25976..27079
                     /gene="livJ"
                     /locus_tag="OO130_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HCR5364889.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(27278..27661)
                     /gene="panM"
                     /locus_tag="OO130_001423"
     CDS             complement(27278..27661)
                     /gene="panM"
                     /locus_tag="OO130_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HCR5364890.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
BASE COUNT         6596 a         7231 c         7745 g         6296 t
ORIGIN      
        1 ccaaatacaa aataatggca aatcatcatg ggataatttt tgatgagcag cgaaaacgtt
       61 acattatagg atcgataaca gtctctattg atgaaatgac caatgctctc gatccggtcg
      121 aattagcgga aattattaat aaaaagcacc atgctgtaat taatggcaaa aagtttaaaa
      181 atagccgggc tatctcttgt cgatccttta atcgttattt ttctgtttcg acagattaca
      241 gacctaaact tgaagcacta ttagcatgct cacaaatgtt aggtataaat caagttgttg
      301 cacataacgg taatgatgga agagaatgta taggtgaaac ggggacagtt ttaggtctta
      361 acgcccgtga cagtaaacat gctggaagaa tgttcagcat gcataattgc caaattaacc
      421 ctggtgcagg gcctgagata accactccct ggaagtctta ccagcatgaa aaaaacagaa
      481 atggacttat gcctttgatt agaaggcgca caatgttaca gctctgaacc atagacaaca
      541 tgaatattaa tgttactatc tggttgagac tgttgataaa acgtaaaaag gaatgctctg
      601 ttcctgcaac tgaacaggcg tgaaacgcga tccaacagga tggataccgt tttgtcagcc
      661 agttttatag atcacactta tgagtatgcg tgtttcattt acggccagga atgctgatga
      721 tgacaacatt actacccgtt ttcactaagc cttccccgtt ggcgctcaat gctctgcgcg
      781 ctggtcgtat ttgtcgtttc cttcttatcc cggatggaag aatccgctga acgtcgttcg
      841 gcggcagttg taacacgcgt cgtttgctta cagggattat ggcgggccga ttatggataa
      901 gagtaagcgc catctggcgt ggtgggttgt cggggcactg gcggtggctg ccgtcgtggc
      961 gtggtggctg ttgcgcccgg cgggtgtgcc ggaaggcttt gctgtcagca atgggcgcat
     1021 tgaagcgacg gaagtggata tcgccagcaa aattgccggg cgtatcgaca ccattctggt
     1081 gaaagaaggc cagtttgttc gcgaaggtga agtgctggcg aagatggata ctcgtgtgtt
     1141 gcaggaacag cgactggaag ccatcgcgca aatcaaagag gcacaaagcg ccgttgccgc
     1201 cgcgcaggct ttgctggagc aacgacaaag cgaaactcgc gccgcgcagt cgctggttaa
     1261 tcaacgccag gcagaactgg actccgtagc aaaacgtcat atgcgttccc gttcactggc
     1321 ccaacgaggg gctatttctg cgcaacagct ggatgacgac cgcgccgccg ctgagagcgc
     1381 ccgagctgcg ctggaatcgg caaaagctca ggtttctgct tctaaagccg ctatagaggc
     1441 ggcacgtacc aatatcattc aggcgcaaac ccgcgtcgaa gcggcacaag ccactgaacg
     1501 gcgcattgcc gcagatatcg atgacagcga actgaaagcc ccgcgtgacg gacgcgtgca
     1561 gtatcgggtt gccgagccag gggaagtgct ggcggcaggc ggtcgggtgc tgaatatggt
     1621 cgatctcagc gacgtctata tgactttctt cctgccaacc gaacaggcgg gcacgctaaa
     1681 actgggcggt gaagcacggc tgatcctcga tgccgcgcca gatctgcgta ttccggcgac
     1741 catcagtttt gtcgccagcg tcgcccagtt cacgccaaaa accgtcgaaa ccagcgatga
     1801 acggctgaaa ctgatgttcc gcgtcaaagc gcgtatccca ccggaattac tccagcagca
     1861 tctggaatat gtcaaaacgg gcttgccggg cgtagcgtgg gtgcgggtga atgaagaact
     1921 tccgtggcct gacgacctgg tggtgaggtt gccgcaatga cgcatctgga actggttccc
     1981 gtcccgcctg tcgcgcaact ggcgggcgtg agccagcatt atggaaaaac cgttgcgctg
     2041 aacaatatca ctctcgatat tccggcccgc tgtatggtcg ggctgattgg cccggacggc
     2101 gtcgggaagt cgagtttgtt gtcgttgatt tccggtgctc gtgtcattga gcagggcaat
     2161 gtgatggtgc tgggcggcga tatgcgcgac ccgaagcatc gccgcgacgt ctgcccgcgc
     2221 atcgcctgga tgccgcaggg gctgggcaaa aacctctacc acaccttgtc ggtgtatgaa
     2281 aacgtcgatt ttttcgcccg cctgttcggt cacgacaaag cggagcggga agtgcgaatt
     2341 aatgagctgc tgaccagcac ggggttagca ccgtttcgcg atcgtccggc agggaaactc
     2401 tccggcggga tgaagcaaaa acttgggctg tgctgcgcgt taatccacga cccggaactg
     2461 ttgatccttg atgagccaac aacgggggtt gacccgctct cccgcgccca gttctgggat
     2521 ctgatcgaca gtattcgcca gcggcagagc aatatgagcg tgctggtcgc caccgcctat
     2581 atggaagagg ccgaacgctt cgactggctg gtagcgatga atgccggaga agtgctggca
     2641 accggcagcg ccgaagagct gcggcagcaa acgcaaagcg ccacgctgga agaagcattt
     2701 attaatctgt taccgcaagc gcaacgccag gcgcatcagg cggtagtgat cccaccgtat
     2761 caacctgaaa acgcagagat tgccatcgaa gcgcgcgatc tgaccatgcg ttttggttcc
     2821 ttcgttgccg ttgatcacgt caatttccgc attccacgcg gggagatttt tggattcctt
     2881 ggttcgaacg gctgcggtaa atccaccacc atgaaaatgc ttaccggact gctgcccgcc
     2941 agcgaaggtg aggcgtggct atttgggcaa ccggttgatc caaaagatat cgatacccgc
     3001 cgtcgtgtgg gctatatgtc gcaggcgttt tcgctctata acgaactcac cgtgcggcaa
     3061 aacctggagt tacatgcccg tttgtttcac atcccggaag cggaaattcc cgcgcgagtg
     3121 gctgaaatga gcgagcgttt taagctcaac gacgttgaag atgttctgcc ggagtcattg
     3181 ccgctcggca ttcgccagcg gctttcgctg gcggtggcgg tgattcatcg cccggagatg
     3241 ttaatcctcg atgagcctac ttctggtgtc gatccggtgg cgagggatat gttctggcag
     3301 ttgatggtcg atctctcgcg ccaggacaaa gtgactatct tcatctccac ccactttatg
     3361 aacgaagcgg aacgttgcga ccgcatctca ctgatgcacg ccggaaaagt gcttgccagc
     3421 ggtacaccgc aggaactggt tgagaaacgc ggagccgcca gtctggaaga ggcatttatc
     3481 gcctatttgc aggaagcggc agggcagagc aacgaagccg aagcgccgcc cgtggtacac
     3541 gacaccaccc acgcgccgcg tcagggattt agcctgcgcc gtctgtttag ctacagccgc
     3601 cgcgaagcgc tggaactgcg acgcgatcca gtacgttcga cgctggcgct gatgggaacg
     3661 gtgatcctga tgctgataat gggttacggc atcagtatgg atgtggaaaa cctgcgcttt
     3721 gcggtgctcg accgcgacca gaccgtcagt agccaggcgt ggacactcaa cctctccggt
     3781 tcccgttact ttatcgaaca gccgccgctc accagttatg acgagcttga tcgtcggatg
     3841 cgtgcgggcg atatcacggt ggcgattgag atcccgccca atttcgggcg cgatatcgcg
     3901 cgtggtacgc ctgtggaact cggcgtctgg atcgacggag cgatgcccag tcgtgccgaa
     3961 acggtaaaag gttacgtgca ggcaatgcac cagagttggc ttcaggatgt ggcgagccga
     4021 caatcgacac ccgccagcca aagcgggctg atgaacattg agacgcgtta tcgctataac
     4081 ccggacgtaa aaagcctgcc agcaattgtt ccggcggtga tcccgcttct gctgatgatg
     4141 atcccgtcaa tgctaagcac ccttagcgtg gtgcgggaaa aagagcttgg gtcgattatc
     4201 aacctttacg tgacccccac cacgcgtagt gaatttttgc ttggtaaaca gttgccatac
     4261 atcgcgctgg ggatgctgaa ctttttcctg ctctgcggcc tgtcggtgtt tgtgtttggc
     4321 gtaccgcata aaggcagttt cctgacgctc accctggcgg cgctgctgta tatcatcatt
     4381 gccaccggaa tggggctgct gatctccacc tttatgaaaa gccagattgc cgccattttc
     4441 ggaacggcga ttatcacgtt gatcccggcg acacagtttt ccgggatgat cgatccggta
     4501 gcttcgctgg aagggcctgg acgttggatc ggcgaggttt acccgaccag tcattttctg
     4561 actatcgccc gcgggacgtt ctcgaaagcg ctggatctga ctgatatgtg gcaacttttt
     4621 atcccgttgc tgatagccat cccgctggtg atgggcttaa gtatcctgct gctgaaaaaa
     4681 caggagggat gatgcgccat ttacgcaata tttttaatct gggtatcaaa gagttgcgca
     4741 gtctgctcgg tgataaagcg atgctgacgc tgattgtctt ctcgtttacg gtgtcggtgt
     4801 attcgtcagc gaccgttacg ccaggatcgt tgaacctcgc gccgatcgcc attgccgata
     4861 tggatcaatc gcagttatcg aaccggatcg ttaacagctt ctatcgtccg tggtttttgc
     4921 caccggagat gatcaccgcc gatgagatgg atgccggact ggacgccgga cgctatacct
     4981 tcgcgataaa tattccgcct aattttcagc gtgatgtcct cgccggacgc cagccggata
     5041 ttcaggtgaa cgtcgatgcc acgcgcatga gccaggcatt taccggcaat gggtatatcc
     5101 agaatattat caacggtgaa gtgaacagct ttgtcgcgcg ctaccgtgat aacagcgaac
     5161 cgttggtatc gctggaaacc cggatgcgct ttaacccgaa ccttgatccc gcgtggtttg
     5221 gcggggtgat ggcgatcatc aacaacatta ccatgctggc gattgtattg accggatcgg
     5281 cgctgatccg cgagcgtgaa cacggcacgg tggaacactt actggtgatg ccgataacgc
     5341 cgtttgagat catgatggcg aagatctggt cgatggggct ggtggtgctg gtggtatcgg
     5401 gattatcgct ggtgctgatg gtgaaaggtg tactgggcgt accgattgaa ggctcgatcc
     5461 cgctgtttat gctgggcgtg gcgctcagtc tgtttgccac cacgtcaatc ggcattttta
     5521 tggggacgat agcgcgttca atgccgcaac tggggctgct ggtgattctg gtgctgctgc
     5581 cgctgcaaat gctttccggt ggatccacgc cgcgcgaaag tatgccgcag atggtgcagg
     5641 acattatgct gaccatgccg acgacacact ttgttagcct cgcgcaggcc attctctacc
     5701 ggggtgccgg attcgaaatc gtctggccgc agttcctgac gctgatggca attggcggcg
     5761 cgtttttcac cattgcgctg ctgcgattca ggaagacgat tgggacaatg gcgtaaggaa
     5821 gggaaacttg acttcatact gtgatgtcat actattgatg tcataaagtg aagtcataag
     5881 gacatgccat gagaaccaag gtacaggctt tacggaagaa acaaaaaata cattggatca
     5941 aatatttaaa actcctgttc ctcaagggat caagtggtct gatatagagt cactggttaa
     6001 agcattaggc ggagaaatta aggaaggaag aggttcgcgt tgtaagttca tactaaatat
     6061 gagcgttgcg tgtttccatc ggcctcatcc gtcgccagat accgataaag gcgctgtaga
     6121 aagcgtgcgt gactggttac taagtatagg ggtaaaacca tgatcaaact gaagacgcca
     6181 aactctatgg agattgccgg gcaaccagct gttattacct acgtgccaga actgaatgca
     6241 tttcgtggca aattcctggg cttgtcaggt tattgtgatt ttgtctctga cagcattcag
     6301 gggctgcaaa aagaagggga actttccctg ctagagtatc ttgaggattg taaagctgcg
     6361 ggtattgagc cgtatgcgcg tacagaaaag attaagacct ttacgttacg ctatccagaa
     6421 tcgctgagtg aacgccttaa taatgcggct gcgcagcaac aagtctctgt taatacctat
     6481 attattgaaa cactgaatga gcgtttgaat catttataaa ttttagcggc gtacattacg
     6541 cagaatatct gcctttacag gatctatcta tgcaactgaa cttaatttta taaattaagt
     6601 tcagttaata tcttttcttt cctcgtttca gaaataactc aatcttcctt cggcaagcac
     6661 tgcaaatgcc catgccgcac gccgcgctgg gcgataacgt catcggcaaa atgctgcacg
     6721 tcgcccatat cacctttcag cacggcgatt tccagacagt cgtcgtgatt gatatgcaca
     6781 tgcagcgtgg cgacggagag atcgtggtga tggtgctggg tggagacaat gcggctggct
     6841 aagtcgcgtt tttcgtgttc atacacatag gagagcaccg cgaaaccttg cgtgccgtgc
     6901 tgctgagtgg cctcctgggc cagggcgcta cgcagaatat cgcggatagc ttcggaacgg
     6961 ttgttataac cacgacgctg gctcaggctg tccagcgtct ccagtaaatc gtcatcaagc
     7021 gtgatggtga ctcgttgcat ttgcgttaaa ccttttctgt ggcgcgacgg cgcacgggga
     7081 atgcgggtaa taccgcgttt tgtagcacac gtccggcgtc agaggaaaag gttaatttat
     7141 ctcccaccgc ctgggtttcg acgatttgtc cgttgtccat caccatcacc cgctggcaaa
     7201 agcgttctac aagacgtaag tcgtgggtga tgaacaggca ggcggtgcca aactgttgtt
     7261 gcagcttttt cagcaggcga atgacgcccg cctgtaacac gagatcaagg ttagaaacgg
     7321 tttcatccag aatcagcaat ttcggttcga ccgccagcgc gcgtgccagg cagacgcgct
     7381 ggagctggcc gccgcttaac tgcggtgggc gtttgtcgag aacgctgtca tcgagatcga
     7441 ccgccttcag catttcgctg gcgcgcgcca gttgttcgga ttttttcagt gatagcaggt
     7501 ggcgcatcgg ttcacgcagg atctcgcgca cggttttgcg cggatttacg gcactgatgg
     7561 agtcctgaaa caccatctga atatcacggc ggaaggcttt acgttgggcg cgattaagtt
     7621 tcgccagcga ttcgccgcgc cagctaatat tcccttgtga gggagactct aagcctacca
     7681 gcagccgcgc gagggtgctt tttccgcagc cgctgcgtcc cagcagggcg acggtttcgc
     7741 cgcttttcag ggtcagggaa acgttattca gcaccgcctg atgttgatgt tttccgctaa
     7801 atccaccgtg ggcatagtga tgggaaaggc cgctgacgtt aagtaaggtc atgatgccag
     7861 ctccataccg tagagggcga gatgagcgga aaccaggctg cgcgtcaccg tatgtttggg
     7921 ggcgttaaac agcgtttcta catcgccctg ttcgacaatt ttaccttgtg acatcaccgc
     7981 cacgtcatcc gccagacgcg ccacaacgcc catatcatgg gtgaccagca gcattcccgg
     8041 cgcttgtttt tgcataatgc tttccagcag atcgaggatg cgcgcctgtg ctaccacgtc
     8101 gaggtcggtg gtcggttcat cggcgatgat aaacggtgat tcacacagca ccgccatcgc
     8161 aatcatcatg cgctgcaaca tgccgccgct catctcgaac gggtacagct tcagcacgcg
     8221 cgcggcgttt tccagcccca ccgcttctat ggcagcggta agcgtagcgt catcggcggg
     8281 tttccctaac gccaggcagg tttcccgcgc gtgggtgtgc atggtgtgta gcggattaaa
     8341 ggcgctgcgc gggttctgca tgatggtggc aattttgatg ccgcgcaggg cgcagggata
     8401 aaccgctttg ccatcggcta aaatttcccc cgccgtctgg cgaacgccag cgggcaaaat
     8461 gcccagcgtc gcggcgcagg ttaatgattt cccgctgccg ctaccgccga ctaacgccag
     8521 cacgcgcccg cgttgcaggg ttaacgatac accgtgtacc agcggctgcg cggcctgtag
     8581 cgcgatatta cgtagttcaa tctgttgcgg cattagtgtg cgtgctccgt caccagatga
     8641 gggtccagat gatcgcgcag tgcgtcaccc accaggttaa aggccatcac gctgataaac
     8701 agcgccagcc ccggccagaa catttgcagc ggctgggtcc agatatactg gcgcgcgtcg
     8761 ttaatcatca cgccccattc ggcggtcggc gcggtcacac cgaggccaag gaaagacatc
     8821 cccgagacgt gcagcatcat atggccgata tccagcgttg ccagcaccag cagcgaaggg
     8881 atcaccgcgc ctgccagatg atcgacaaac actcgcacat ggcccgcacc ggaaagccgt
     8941 gacgccagca caaactcgcg ttggcgtagt gaaatcacca ggctgcgcac catgcgtgca
     9001 taccacgccc agtgcgacag ggcgatggcg ataattacgt tggtcagccc ggtgccgagc
     9061 acgccaacca taaagaacga cagaatcgag gtcgggaagg tcataaacat atcggcgacg
     9121 cgcatggtgg cctgatcaac gcgcccgcca atcaacccgg cgctgccgcc aataaccagc
     9181 cctaatgtca gcaccagcag caggcaggcc attaccgaac cgagcgacac gcgggtcgct
     9241 gccatcagcc gcgagaaaat atcgcgacct aagtgatcgg tgcccagcca gtgctgcgca
     9301 tccggcgaaa gcaggtgcga cggcaaatca atcgcctggg ggtcatacgg cagccaccac
     9361 tggctggtga gcgcaatcag cgccagcagg gcgataatga tcagcgccag gcgtaccgac
     9421 cagcgggaag agaggaaaaa gttcacgcgt gcgctccttc atgacgacga atgcgcgggt
     9481 ccagcgcggc gttgagcaaa tcgacaatca aattacagac cacaaaaacc accaccatca
     9541 tcagcgtaaa gaactgaatc accggatagt cacggttaaa aatcgccgac accgcatagc
     9601 gcccgacgcc aggccaggca aagatgtttt cgataatcat cgtcccgcca atcaattcgc
     9661 caatgtgcat ccccacggcg gtgatcatcg gcagcgaggc attgcgcaga atatggcggc
     9721 gttcggtctg tttgtcattc aggccgcgta gccgcgccca ggtgacgtga cgctgaccgg
     9781 cgacgtccag catactggcg cgcagcagtc gcgcgttaat cgccagcgac ataaaggcga
     9841 tggaaaccgc aggcaaaatg atgtgctgcc agccgccgta gcccatcgcg ggtagccatt
     9901 gcagatacac cgaaaacgcc atcactagca aaaatgccag ccagaagttg ggcatcgaca
     9961 cgccaagaaa cgcgataaag cgcacggcga aatccggcaa acgatcgcgg tggcgcgccg
    10021 cccagatacc gagcggtacg gaagtgagca gaattaatac cagcgccgca cctgcaagtt
    10081 ccagcgtggc gggcaggaag ttcagcatat cgtccagcac cgggcgttgg ctggcgaatg
    10141 agataccaaa gtcaagatgc agcgccttcc acaaccaggt gccgtactgg acgtacagcg
    10201 gctgatccag tccgagcatg gtgcgggtag aggccagcat ctccggcgtc ggcggcaggt
    10261 tagacagacg caaataatcg agcgccgggt cgccggtacc gaggcgcagc atcagaaaaa
    10321 tgatcaccga ggcggcgagc accatcggga tcagcagcag aaagcggcgt aatacgtaac
    10381 gcaacattaa ggtttcaccg gtttaatctg ttcgaacgga atttcggtgg cgatcggcgc
    10441 gtaggggatg ttacccagct ccggttttga taccaccatc attgagatgt aactgatagg
    10501 cagataaacc gcctcgtcat gcagacgggt cagaatgtcg cgatacagcg cctgacgttg
    10561 cgtttcgtca tgggtcgcca gcacttcgcc gatctcttta tcaatcagcg gtttgtcggc
    10621 taatccttgc tgtgcctgga agtcagcgtg cgacggtacg cgcattgaac tcaggaaggc
    10681 gtgtggatcg tatggcgcgc cccaggtgcg gtggaaaatc atgccaaaac gaccgtcgcg
    10741 ctgacgagca tagatactgc tctcttcttc gccaatcagc gagacatctg cgccaatctg
    10801 gcgcatatca gcctgaatga tttccgccat cgatttgctt aacgcatcgg tgccgatgaa
    10861 cgacagttca atgcgcagcg gctgaccatt tttctcgcgg atgtctttgc ccgcaggcag
    10921 cgtccaaccg gctttttcca gcaacgcttt cgctttctgc ggatcgtact ggcgcggttt
    10981 caggccgagg ttggcgtagg gcacagaagg ggcaaacagg gtgtcggcga cctgctgggt
    11041 gccatacaac gcgttatcaa tcagcgattt tttgtttacc gcgtaattaa gagcttcacg
    11101 tactgccagc tcattggtgg gggttttggc ggtattaagc gccagcatca cggtttcgat
    11161 cggctgtgac agttgggtat ggtaagccgg gttctggcta aagcgggcga aggtatcgag
    11221 cggtaataac ccttcgtttc cgtacagcag gtcgatatcg ccagtttcaa acgccaccgc
    11281 gcgggtagtc gggtccggga tgacgttaaa ggtgatcttt ttaatcgctg gcttttcgcc
    11341 ccagtagttt tcgttacgga cgaagacatc gtactgattc agtttcgatt cctgcaaaat
    11401 ccacggtccg gtgccaatcg gcgctttaat tccgttcatg gtttcatggt ttttaaactg
    11461 cgagggagcg ataaaacgga aaggacgggg cagggccagt tcttgcagga aaggatagta
    11521 ggcgcttttc agggtaattt gcagctctgt tttactgagt gctttaacat caacaatctg
    11581 gtttgccagc tccagccagg cgtgacgttg acggttatcg agcactgcgc ggaagttttc
    11641 tgccgccgcc tcggcatcga acggttcacc gttggataat ttcacgtcat cacgcagggt
    11701 gaaggtccag gttttaccat cttctgaatg agtccagctt tttgccagcc acgggatcac
    11761 cgaaccgtct gcctgatatt tcaccaatgg ttcataaacc atgctctggg cgaacatctg
    11821 gttaggcgtg taaaggtgcg ggtttagtgg cccgacattc accggccagg cggtagtgat
    11881 ttcatctggt gcagcggcat ggacgataaa agacgcacaa gccagcagcg caaatagagt
    11941 gcggcggagt gtggagagca tgataacccc aatggattaa aatagatggc ggaaataagt
    12001 atgacgattt taagtattcg tcatactgat tacctgttct ggatcaatag atgggcagtc
    12061 gggtgggcgg atcagttaac tgaatcgatc cattgcacac tgtcggcggt gagcgtaaag
    12121 ggggtggggg tgcagatcgc aaggcttaaa ttttctagct gacaatggct gactgacagc
    12181 gaggagtgat aggtgctatc tacgctgacg atttgccagg cgctgccgcc acgctgttta
    12241 acgatggctt ctttgcgcgt ccagatgcgc cagaacattt ccagttgctg atcaggatgc
    12301 acggcgtcca tttcagcgtg ttccccgagg ctgaacacgg cgttcgccag ccagcgccag
    12361 ttggcgcgcg ggcgaatcac ttcgatatcg cagccgactt cgccttcatc gctcaacagc
    12421 agagcgatat cgtcaccgct atggcttaag ttgaaccata gcggcgtttc cggcgcgaat
    12481 gcaggtttgc cttgttcgcc atagatgatc tccggtagcg gggaaagcgt gtgcgaaagc
    12541 aatgcacgcc ccgccagcca gcgttcgcgt cgtggacctt gcggtgcttg ctcgcgtaaa
    12601 cccggtggca gtggagctgc gcttaaggtc gaaactttcc ccagaactat ccgatacata
    12661 tcagggccaa cgtttaatgg aaaatgaaag tgcgtatcgt atcacttgtc gcctcatccc
    12721 ggtaaccgac ttttcggtct gcctggcccc agtaaaatcg ccagtttgct accgcctttg
    12781 gtggtttcca tccagatttt acacacgctg gttaatggca ccgaaagtag catccctacc
    12841 gggccgagca gccatcccca aattaacaat gaaagaaata ccaccatggt ggacatcccc
    12901 aggcgatggc ccatcatccg tggttctaaa atattgccga tgaccatatg gaccactaaa
    12961 aacaatgcgc cgaccagaat acatgcgtaa acaccattaa acagcagcac ctgaatcatt
    13021 ggcggtacgg cggaaattac cgcgccgata ttgggcacgt agttgagcaa aaacgccagt
    13081 actgcccaca tcagcgcaaa ctgtacgccc attagcgcca gccccagcca gacgatgaca
    13141 cctgtccata aactgagtag cgtctttaac gccagataat gcgaaacgcc tttaagtgcg
    13201 cggtgtaatc ctgcgatgtg aatctgtggg ttattcagcg caaaacgcat tttgtaaggg
    13261 acgtggcgca cttcaaacag cataaaaact acggtcatca ccagcaaaag cacgctcgcc
    13321 attgccccgg aaagcccgat cattagcgct gtggtgaagg taaccacttt ttccgagtcc
    13381 atccgctgca gcattcgctc cggcgacata tgcaaattaa gaaaaggcaa catctcctgc
    13441 aatttaaaaa gtttgcgcgt cagctcctta ttaaacttcg gcagcataga gataaattcg
    13501 ttaaacgatg ccgccagtac gccgactagc gcggttagtg cgatcagcat caccaccact
    13561 acaatcgtaa tggtaacggg gcgttgtact ccccgacgaa taaaccaggt aacgagcggg
    13621 ttgaggacga tggcaaaaaa cagcgccagt aacagctgca caatgatatc tgccgctgcg
    13681 tgaatgcccg cgaggatcac taccagcgag gccaacttga gcagaatgtg catgcccgtt
    13741 ttatcgggtt gaggggtttc cattggggct tccttgtgac tttttgtgtt aagtgtagcg
    13801 ggagtcacgt cagcaatatt ctgattctta ctgctgattt tcacgtcagc acgtggtaaa
    13861 aatgaaacac tgttgtaaaa atgtggtgat cctcatgccc gaacccgtag ccgaacccgc
    13921 gctaaacgga ttgcgcctga atttgcgcat tgtctccatt gtcatgttta acttcgccag
    13981 ctacctcacc atcgggttgc cgctcgctgt attaccgggc tatgtccatg atgtgatggg
    14041 ctttagcgct ttctgggcag gattggttat cagcctgcaa tatttcgcca ccttgctgag
    14101 ccgtcctcat gccggacgtt acgccgattt gctgggcccc aaaaagattg tcgtcttcgg
    14161 tttatgcggc tgctttttga gcggtctggg atatctgacg gcaggattaa ccgccagtct
    14221 gcccgtcatc agcctgttat tactttgcct gggacgcgtg atccttggga ttgggcaaag
    14281 ttttgccgga acgggatcga ccctgtgggg cgttggcgtg gttggctcgc tgcatatcgg
    14341 gcgagtgatt tcgtggaacg gcattgtcac ttacggggcg atggcgatgg gtgcgccgtt
    14401 aggcgtcgtg ttttatcact ggggcggctt gcaggcgtta gcgttaatca ttatgggcgt
    14461 ggcgctggtg gccattttgt tggcgatccc gcgtccgacg gtaaaagcca gtaaaggcaa
    14521 accgctgccg tttcgcgcgg tgcttgggcg cgtctggctg tacggtatgg cactggcact
    14581 ggcactggct tctgccggat ttggcgtcat cgccaccttt atcacgctgt tttatgacgc
    14641 taaaagttgg gacggtgcgg ctttcgcgct gacgctgttt agctgtgcgt ttgtcggtac
    14701 gcgtttgtta ttccctaacg gcattaaccg tatcggcggc ttaaacgtag cgatgatttg
    14761 ctttagcgtt gagataatcg gcctgctact ggttggcgtg gcgactatgc cgtggatggc
    14821 gaaaatcggc gtcttactgg cgggggcagg gttttcgctg gtgttcccgg cattaggtgt
    14881 agtggcggta aaagcggttc cgcagcaaaa tcagggggcg gcgctggcaa cttacaccgt
    14941 atttatggat ttatcgcttg gcgtgaccgg accactggct gggctggtga tgagctgggc
    15001 gggcgtaccg gtgatttatc tggcggcggc gggactggtc gcaatcgcgt tattactgac
    15061 gtggcgatta aaaaaacggc ctccggaaca cgtccctgag gccgcctcat catcttaaaa
    15121 cttactgaat aaccagcgta ttaatgatgt tttctgcggt ggtctgcgct ttttgctgat
    15181 cgtcagcggg cagcgtaatt tgcatggtca gcagttgatt acccacgtta cccagaataa
    15241 cggaagagta cgccgtctgg cctttcgcgg agataatact gtctaactgc tgcattttgt
    15301 gacctttcag ctcaatggct ttattggtta ccacttgcag ctgcggatcg cggctacgtt
    15361 gctgatcttc cagacgcttc gccagcaccg ccagatcttc tttcggatca tcgcccatga
    15421 tgacgatgac tgctttctgc ccggtggcgt cggaccagac atgcatgtta ttggcctgcg
    15481 ttcccagctt accgctctgg tcggtcatat ccgctggcag cgagaaactt aacttgccat
    15541 caagcaggtt gacgggattc ccggtagcgt tattttccgc gaccgaaccc tgcgccgtag
    15601 cgttagtgtc tttatcatca caggccgcaa gccccataac cagcaggcca attccgacat
    15661 atttaaccag attgcgcatt gacttcttcc tttcgataaa cggccataac ggctcattca
    15721 tccatcttat cacaactctg ataacgaacc tttaactcgc ctgcaaagcg ttgatttcgg
    15781 atttatctgc cagtcttttc aacagcatat tgagtaatac gccatacatt ggcaggaaga
    15841 aaacgatact gattaacact ttgaaacagt aatcgaccag cgcgatttcc atccagtgtt
    15901 cagccataaa ggcatccggg ctacgccaga aggcaatgaa gaaaaaggcc agcgtgtcgc
    15961 tgacgttacc gaacagtgtg gacgctgtcg gtgccagcca ccagcggcga ctctgacgca
    16021 ggcggttaaa aacgtgcacg tcgaggatct gtcccagcgc gtaggccatg aaactggcgg
    16081 tggcgatacg ggcgacaaac aggttgaagt gggcgagtgc gccgaatccc tgccaggaac
    16141 ccatatagaa tagcgacgag atgacgtagg agatcagcaa cgccgggatc atcaccgcaa
    16201 agatgatacg acgggccagc ggtgcgccaa aaatacgcac ggtcaggtcg gtagcaagga
    16261 aaataaacgg gaagctaaac gcgccccagg tggtatggaa acccaaaatg gagacgggaa
    16321 gctgaaccag atagttactg gaggtgatca ccagcagatg aaataacgat aaccagaaca
    16381 acgccttata gcgttgagtt tgcgagaaaa cgttcatatt gtaccttttt gattaaccat
    16441 tggggtgagg gaacccaata cgtacgacac gtctgttacc ttaacataat aacgtgctta
    16501 cccttttttc gagccgccgc atgatactgc tttacgttga caatgcaatg gttaattttc
    16561 acgcaatcgt taacctggtt tgcttacgga ctcacagggc gtaaactagc gccgtttttt
    16621 tatgtgatga gaagaaaatg accgatctct tttccagccc tgaccacaca ctcgacgcac
    16681 ttggcctgcg ctgcccggaa cccgtaatga tggtgcgcaa aactgtgcgc aatatgcagc
    16741 ccggcgaaac gttgctgatt atcgccgacg atccggccac tacccgcgat attcctgggt
    16801 tttgtacctt tatggaacac gaactggttg cgaaagagac ggatggactg ccttatcgtt
    16861 atttgattcg taaaggcggt tgatgggggc tgattggctt cgatgccgcc ttttcccctc
    16921 accctaaccc tctccccaga ggggcgaggg gaccgatcgc gctcaatgtt gcgatcggtt
    16981 tgccttatct cctgcgcaac aatcttaacg cattcgctgt caccagcacc gtcgcacccg
    17041 tatctgccag caccgccagc catagtccgg tcatccctaa aagcgtggtg acgaggaaga
    17101 tccctttcag tcccagcgca atggtgatgt tctggcggat attggcgtga gtggcgcgtg
    17161 ccagttcaat catttgcacc aggccgcgca ggtggttatg ggttaatgct gcatcagcgg
    17221 tttccagcgc cacgtctgtg ccactgccca ttgcaatccc aatggcggca gctttcatcg
    17281 ctggcgcgtc gttaataccg tcaccgacca tcgccagcgg cgcatgttga ttcagctcgg
    17341 tcaccgcttt gactttatat tccggcaaca ggcccgcttt aaattccagc cccagctccc
    17401 cggcaattgc cgccgctgcg cgtggattat cgccggtgag gatcacccct ttgacgccca
    17461 gcgcgttcag ttcactgatg gcagttgcag catcggcgcg cagggtatcc tgcaatgcaa
    17521 tgatacccag cacgtcatca ttacgtacta ccagcacaac cgtttgcccg gcgctttcca
    17581 gttcattaat caaaccagca aatgcatcag cgggatgttt cccggcagcg catatcaaca
    17641 cgcgctcacc gttaacctgc gcttcaatgc cagacccgac cagcgcccgc tgtgattcgg
    17701 cggcgggaat ggcgagtgca gcaacctgtg cttcgcgcac gatggcctgc gccagtggat
    17761 gcgtcgcgct ttgctcgacc gccgccgcca gtgtcagcag ttcagattca ctaatacccg
    17821 ttgccggatg aatcgcggta acgcgcggtt taccgacggt cagcgtaccg gttttatcaa
    17881 acgccacctg agtaacacga cctagttgtt ccagcgccgc tccgccttta atcaacgccc
    17941 cacgacgcgc tgctgccgcc agcccggagg taatcgccgc gggcgtggag ataactaacg
    18001 cacacgggca gccaatcagc agcagcgtca ggcctttata aatccactcc tgccagctgg
    18061 cggcaaacag cagcggtggc accagcgtca ccagcagggc gacggccata atcgctggcg
    18121 tataaatacg gctgaaacgg tcgataaacc gctcaatggg ggcgcggcgc tcttcggctt
    18181 cttcgatcag tttcagaatc cggtcaatgg cgctggcacc cggttctgac agcacttcca
    18241 gcgttaccag acggtctacg ctggtggctc ctgcaggaac tttatcgccc gtcgcgcgct
    18301 ccaccggaat ggattctccg gtcagggcgc tttcatcaaa actggcaaac ggtgagagca
    18361 gtttaccgtc ggcaggcaaa cgcccacctg cggcgacttc aatcacatcg ccaggccgca
    18421 ggctgttaat cgccacctct tcccgctcac cgttacgcag gcgcgtggcg gtttctggtt
    18481 tcagcgccat taacgcgcta acgccctgac gcgcgcggct ggcggcccag ccttccagtc
    18541 gttcaccaat caaaaacagc agcaacacca tcgcagcttc agccgttgcg ccaataaaca
    18601 gtgcaccaat agcggctacg ctcattaagg tttcaatggc gaagtagctg ccggatttga
    18661 tcagccgtaa tgcctgatga gcaatcgggt acagcccaac cagcgtggtc gcgataaacg
    18721 ccagttgccc gaacggatga ttgaactgct ccagacccca gctgattgcc atcatcacga
    18781 ttagtgtaat cagcggcaga ttctctttca ggcgcgatgc ttgtggctcg tcggcggcct
    18841 gctcatcgcg cagggaatag cccgcttttt gcaccgcaga ttcaacttgt gcacgaatgt
    18901 cattgtcggc atcaaccacc agtttttcgg tggcgaacaa cacctgcacc tgattcacac
    18961 ctgcaagctg gcgcacggca ttttctacct tgcgcgcaca ggcggcgcag tccatgccac
    19021 tgactttcca gctatagcgg gtgccggaga cgttttcaga gagcgttggc gagctggagc
    19081 atgcgccgtc gcagcaacag tcgttggcgt tctgtaccgt ggttagcggt ttgaacgcag
    19141 caaattgagg ggctttcttg ccgtgattgt caggtgtcga catggcatac tccggttaag
    19201 ttttttctca ttaaccgaag gatacactct ggagtcgact ccagagtcaa gttttatcag
    19261 aaatacagtg agcggacgat gaggaagtga ccggcaaagt agcaggccgc agcaatcgcg
    19321 ttatccgcac ggaagcgtcg gcgatagtgg ctccccagcc agacaaagtt actgataaac
    19381 agcaacgaag cgccaacaaa cgcagagagc gccggagcgg tcggacggaa gaaccacagt
    19441 tcacctgcca gccacaccat caccagcgtc atgccgataa acgtgcagat aggccaacga
    19501 tactcttcca gccgcgtcca gataatcgcc agtaacagcg cacccagaac cagcaacacc
    19561 agtggcagag gccagaagaa agagagcgtc atctgactgg cgaaatagat ggtgtacagc
    19621 aggtgcgaga ggaaaaacgc gccgatggcg tacatcagac gttgacgtgg caacagggtt
    19681 agcgcatcgc ccagcagtga ggcgcacagc cctgccagca ccagatagct aatggcgtcg
    19741 aacatcggcg cttgccaggc cagtaacagc aggagaagaa gggttaacgg tttaaacacc
    19801 cagcgttgcc aggttggccc acgatacgat gcatccacag atagccatgc ggaaagacag
    19861 acagcgataa acgaccaaag catcttagtt ccttgaattc gttatttttt cgtaagcgga
    19921 atgcctacgg tcttgcttcc agtttagtgg cgagggtgga cggatgacaa caccataact
    19981 agcagggtat gcttatttcc gcattttccg atgagggata aagatgagca aaccacctct
    20041 tttctttatt gttatcattg gcttaattgt cgtcgccgca tcgtttcgtt ttatgcaaca
    20101 gcgacgggaa aaagctgata atgatatggc tccgctccag caaaagctgg tggtggtgag
    20161 caacaagcgg gaaaaaccga ttaacgatcg ccgttcgcgc cagcaggaag tgactccggc
    20221 aggcaccagt atgcgctatg aggcaagctt caaaccgcaa agcggaggaa tggagcagac
    20281 gtttcgcctc gacgcccagc agtaccatgc cctgacagtg ggcgataaag gtacgctgag
    20341 ctataaagga acgcgctttg ttagctttgt tggcgaacaa taaggttatt ttttaacttt
    20401 aaatttcttc tgccagacca gcagttcaaa cacgccgaaa aggaaaatcc gcaccttttc
    20461 ggcggtggtc atctgcgggc catctttcgg taaagtggat ttcaatagcg ccagctgcat
    20521 accatgcatc agcacggtaa aaatcagcgc cacgttaacg aaaatattca gcgggcgcgg
    20581 gaagggatgc accagattga ggattaaaaa tccccaaacg cagagcatta acaaacgacc
    20641 aatattaatc agcatcactt tctccttgtg cttcgcgttg atacagccga taagccacct
    20701 gacccgccac tttttcccga tgtaatgacc agtttgccgg aacagtgggc agaccgtttt
    20761 cgacttcgct ttcgacataa atcagggctt cgtcagccag ccagccgtta tcttccagta
    20821 aatttatcgt ctcttctaac aagccacggc ggaacggtgg atcgacaaac acgatattat
    20881 gcggtgtacc tttttgcgcc aggaatgaca tcgcgttgct gttcaccacg cgtgcattgc
    20941 ctgcttttag tgtcgccaga ttcttaatta actgctgaga aaccgcgcga tccatctcaa
    21001 tcaacgtggc ccccgcagcg tagcgcgata acgcttccag tcccagcgcg ccgctcccgg
    21061 cgaagcaatc cagacattgg gcgtcaacaa tgaccggagc cagccagtta aacaatgttt
    21121 cgcgtacgcg gtcggtggtg gggcgcagac ctgggctatc aggaaccggg agtttacggc
    21181 ctcgccactg cccgccaata atgcggattt ggccgctgcc ggaatgattc ggttttttca
    21241 tgataaattg ctcaatccgc cagataacac atacttgcag gcggtgatgt gaattaagtt
    21301 aagtgataga ctatttcatc atttttttag ctgctatgta catagcgtta acgctgtgcc
    21361 atgaagcaac agcgaggagt gtagtcgcaa atggcgaaag aaaaaaaacg tggctttttt
    21421 tcctggctgg gctttggtca aaaagagcag accccggaaa aagagacaga agttcagaat
    21481 gaacaaccgg ttgtagaaga aatcgttcag gcgcaagagc ctgcgaaggc ttctgaacaa
    21541 gccgtcgaag agcaaccgca ggcgcatact gaagccgagg cggaaacttt tgctgccgac
    21601 gttgtggaag tcactgaaca ggttgctgaa agtgaaaaag cgcagcctga agcggaagtc
    21661 gttgcacagc cggaaccggt cgtagaagaa acgccggagc cagtggctat cgaacgtgaa
    21721 gagctgccgt tgccggaaga cgtcaacgcc gaagaggttt cgccagaaga gtggcaggct
    21781 gaagcggaaa ccgtagagat tgtcgaagcg gcggaagaag aagcggctaa agaagaaatc
    21841 accgacgaag agctggaagc acaggcgctg gctgccgaag cggcagaaga ggcggtgatg
    21901 gtggttcctc cggtagaaga gcagccggtg gaagaaatcg ctcaggagca ggaaaaaccg
    21961 accaaagaag gtttcttcgc gcgcctgaaa cgcagcctgt taaaaaccaa agaaaatctc
    22021 ggttccggat ttatcagcct gttccgcggt aaaaaaatcg acgatgatct gtttgaggag
    22081 ctggaagaac agctgttgat cgccgatgtg ggcgtggaaa ccacacgtaa aattatcacc
    22141 aatctgacgg aaggcgcatc ccgcaagcag cttcgtgatg ccgaggcgct ctatggcctg
    22201 ctgaaagaag agatgggcga gattctggcg aaagtcgatg agccgctgaa tgttgaaggt
    22261 aaaacgccgt tcgtgatcct gatggtgggc gtcaacggtg tgggtaaaac tacgacgatt
    22321 ggtaagctgg cacgtcagtt tgagctgcag ggtaaatcgg tgatgctggc ggcgggcgat
    22381 actttccgtg cagcagcggt tgaacagctt caggtctggg gtcagcgcaa caatattccg
    22441 gtgattgccc agcataccgg tgcggattcc gcctctgtta tcttcgacgc cattcaggca
    22501 gctaaagcgc gtaatatcga cgtcctgatt gccgataccg ccggacgcct gcagaacaaa
    22561 tcgcacctga tggaagagtt gaagaaaatc gtccgcgtga tgaagaaact cgacgttgaa
    22621 gcgccgcatg aagttatgct gactattgat gccagtaccg ggcagaatgc ggtaagccag
    22681 gccaaactgt tccacgaagc cgttggctta accggcatca cgctaacgaa actggacggc
    22741 acggcgaaag gcggggtaat tttctcggtg gctgaccagt ttggtatccc tatccgctac
    22801 attggtgtcg gcgaacgtat tgaggatttg cgtccgttta aggcggacga ctttatagag
    22861 gcactttttg cccgagagga ttaacaatga ttcgctttga acatgtcagc aaggcttatc
    22921 tcggtgggag acaggcgctg cagggcgtta cgttccatat gcagccgggt gagatggcgt
    22981 ttctgaccgg tcattccggc gcagggaaaa gtaccctcct gaagctgatc tgtgggattg
    23041 agcggcccag cgccgggaaa atctggttta gcggccatga catcacgcgt ctgaaaaacc
    23101 gtgaagttcc gtttctgcgc cgccagattg gcatgatttt ccaggatcac catctactga
    23161 tggaccgtac tgtctacgat aacgtggcga tcccgctgat tatcgctggt gccagcggtg
    23221 acgatattcg tcgccgggtg tcggcggcac tggataaagt cgggctactg gacaaagcga
    23281 agaacttccc tattcagctt tccggcggtg aacaacagcg tgttggcatt gcccgcgcgg
    23341 tggtgaataa gcccgcggta ctgctggcgg acgaaccgac cggtaacctg gacgacgcgt
    23401 tgtcggaagg gattctacgt ctgtttgaag agtttaaccg cgttggggta accgtattga
    23461 tggcaacgca cgacatcaac ctgatttcac ggcgttccta tcgcatgctc accctgagcg
    23521 atggtcactt gcatggaggc gtgggccatg aataagcgcg atgcaatcaa tcatattcgg
    23581 cagtttggtg ggcgtctgga tcgcttccgt aaatcggtcg gcggctcagg cgacggcggt
    23641 cgtaacgcac cgaaacgcgc gaaatcctcg ccaaaaccgg taaatcgcaa aaccaacgtt
    23701 ttcaacgaac aggtgcgcta tgccttccac ggcgcattgc aggatctgaa aagcaaaccg
    23761 ttcgccacgt ttttaacggt gatggttatc gccatctccc tgacgctgcc cagcgtctgt
    23821 tatatggtgt acaaaaacgt taatcaggcg gcgacgcagt attatccgtc accgcaaatc
    23881 actgtttact tgcaaaaaac gctggacgat gacgccgctg cgggcgtggt ggcacagttg
    23941 caggccgagc aaggcgtgga gaaagtgaac tatctttctc gtgaagacgc actgggcgag
    24001 ttccgtaact ggtctggttt tggtggtgcg ctggatatgc tggaagaaaa cccgcttccg
    24061 gcagtggcgg tggtgatccc gaaactcgat tttcagggga cggaatcact caatacgctg
    24121 cgtgatcgta tcacgcagat taacggcatt gacgaagtgc ggatggatga cagctggttt
    24181 gcccgtctgg cggcgttgac cgggctggtc gggcgcgttt cggcgatgat cggcgtgttg
    24241 atggtggcgg cagtgttcct cgtcatcggc aacagcgtac gtctgagcat ctttgctcgc
    24301 cgtgactcca tcaacgtgca gaaactgatt ggtgcgacgg atggtttcat ccttcgcccg
    24361 ttcctgtatg gtggcgcact gctgggattt tctggcgcat tgttgtcatt aattttgtca
    24421 gaaattctgg tgctgcgatt gtcatcggcg gttgcggaag tggcacaggt tttcggaacg
    24481 aattttgata tcaatggttt atcgttcgat gaatgcctgc tattgctgct ggtatgctcg
    24541 atgattggct gggtggcagc gtggcttgcc acggtacaac atttacgcca ctttacgcct
    24601 gaataataaa agcgtgttat actctttccc tgcaatgggt tccgtagcag ggaaagagat
    24661 cccgttgtct cttcccggta tttcatctct atgtcacatt ttgtgcgtaa tttattcaca
    24721 agcttgcatt gaacttgtgg ataaaatcac ggtctgataa aacagtgaat gataacctcg
    24781 ttgctcttaa gctctggcac agttgttgct accactgaag cgccagaaga tatcgattga
    24841 gaggatttga atgactgaca aaatgcaaag tttagcttta gccccagttg gcaacctgga
    24901 ttcctacatc cgggcagcta acgcgtggcc gatgttgtcg gctgacgagg agcgggcgct
    24961 ggctgaaaag ctgcattacc atggcgatct ggaagcagct aaaacgctga tcctgtctca
    25021 cctgcggttt gttgttcata ttgctcgtaa ttatgcgggc tatggcctgc cacaggcgga
    25081 tttgattcag gaaggtaaca tcggcctgat gaaagctgtg cgccgtttca acccggaagt
    25141 gggtgtgcgc ctggtctcct tcgccgttca ctggatcaaa gcagagatcc acgaatacgt
    25201 tctgcgtaac tggcgtatcg tcaaagttgc gaccaccaaa gcgcagcgca aactgttctt
    25261 caacctgcgt aaaactaagc agcgtctggg ctggtttaac caggatgaag tcgaaatggt
    25321 ggcccgtgaa ctgggcgtaa ccagcaaaga cgttcgtgag atggaatcac gtatggcggc
    25381 acaggacatg acctttgatc tgtcttccga cgacgattcc gacagccagc cgatggcacc
    25441 ggtgctctat ctgcaggata aatcatctaa ctttgccgac ggcatcgaag atgataactg
    25501 ggaagagcag gcggcaaacc gtctgaccga cgcgatgcaa ggtctggacg aacgcagcca
    25561 ggacatcatc cgcgcgcgct ggctggacga agacaacaag tccacgttgc aggaactggc
    25621 tgaccgttac ggtgtttccg ctgaacgtgt acgccagctg gaaaagaacg cgatgaaaaa
    25681 actgcgcgct gctatagaag cgtaatttcc gctattaagc agagaaccct ggatgagagt
    25741 ccggggtttt tgttttttgg gcctctacaa taatcaattc cccctccggc aaaacgtcaa
    25801 tccccacgca gattgttaat aaactgtcaa aatagctatt ccaatatcat aaaaatgggg
    25861 tatgttttag cagagtatgc tgctaaagca cgggtagtca tgcataaaac gaaataaagt
    25921 gctaaatcac aacaccacaa cacacgtaat aaccagaaga atggggattc tcaggatgaa
    25981 tataaagggt aaagcgttac tggcaggatg tatcgcgctg gcattcagca atatggctct
    26041 ggcagaagat attaaagtcg cggtcgtggg cgcaatgtcc ggtccggttg cgcagtacgg
    26101 tgaccaggag tttaccggcg cagagcaggc ggttgcggat atcaacgcta aaggcggcat
    26161 taaaggcaac aaactgcaaa tcgtaaaata tgacgatgcc tgtgacccga aacaggcggt
    26221 tgcggtggcg aacaaagtcg ttaacgacgg cattaaatat gtgattggtc acctctgttc
    26281 ttcatcaacg cagcctgcgt ctgatatcta cgaagacgaa ggcattttaa tgatcacccc
    26341 agcggcaacc gcgccggagc tgaccgcccg tggctatcag ctgatcctgc gcaccaccgg
    26401 cctggactcc gaccaggggc cgacggcggc gaaatatatt cttgagaaag tgaaaccgca
    26461 gcgtattgct atcgttcacg acaaacagca atacggcgaa ggtctggcgc gagcggtgca
    26521 ggacggcctg aagaaaggca atgctaacgt ggtgttcttt gatggtatca ccgccgggga
    26581 aaaagatttc tcaacgctgg tggcgcgtct gaaaaaagag aatatcgact tcgtttacta
    26641 cggcggttat cacccggaaa tggggcaaat cctgcgtcag gcacgcgcgg cagggctgaa
    26701 aactcagttt atggggccgg aaggtgtggc taacgtttcg ctgtctaaca ttgcgggcga
    26761 atcagcggaa gggctgctgg tgaccaagcc gaagaactac gatcaggttc cggcgaacaa
    26821 acccattgtt gacgccatca aagcgaaaaa acaggaccca agcggcgcat tcgtttggac
    26881 cacctacgcc gcgctgcaat ctttgcaggc gggcctgaat cagtctgacg atccggctga
    26941 gatcgccaaa tacctgaaag cgaactccgt ggataccgtg atgggtccgc tgacgtggga
    27001 tgagaaaggt gatctgaaag gctttgagtt cggcgtattt gactggcacg ccaacggcac
    27061 ggcgaccgat gcgaagtaat cattaatcgg caactttggg ttgccgccaa attgctaata
    27121 tcgagtacgt tgcttcatgc cggatgcggc gtaaacgcct tatccggcct acaagatcca
    27181 aagaaatcag taaattgcaa cacacattgt aggcctgata agcgtagcgc atcaggcaat
    27241 ttctcgttgg tcatcacttt tgaaatcaac ttgacgatta acgcttctcc cagccgccct
    27301 gttgcgccgt aaatcccagt gcctgcataa acgccgtcat cacaccgcga tcttccacgc
    27361 ctgcatccgc catccaccag catgaaacgc caggattgtt acgcaaaacc tcttccagca
    27421 gatattgccc cacaccgcga cgtcgggtga cttcccgcac acgcagggaa tccagtgctc
    27481 cctcggtgcc gcttaaggtt acccgtacgg cagcgagcag gcgctcgtta aaacgcgcgg
    27541 cgtagatacg gtggttatcg tcaacctgta aagaggaagg ggaatactcc ggccagatct
    27601 tttgcaggtc aatccggtcc tggtcgctaa agttttctaa tcgaatgatg gtcagcttca
    27661 tgggtaaccc gtgtaaatca caaaagtgga accagtgtag cgaaataatt taatcggagg
    27721 ctttctcttt tttatttctt ttggcaggtg attaattttt taacagcaat aattacaaag
    27781 ttaaaacatt agagaatgaa aaatacccag cataatcccc tgaatggtag tgaattattc
    27841 cgccctttgt gccgttattt tatgctga
//
LOCUS       DAJTMC010000037        27676 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.037, whole genome shotgun sequence.
ACCESSION   DAJTMC010000037 DAJTMC010000000
VERSION     DAJTMC010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27676
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.037"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(211..420)
                     /gene="fumD"
                     /locus_tag="OO130_001424"
     CDS             complement(211..420)
                     /gene="fumD"
                     /locus_tag="OO130_001424"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HCR5364891.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELDKQAMETVINALVK"
     gene            977..2389
                     /gene="pykF"
                     /locus_tag="OO130_001425"
     CDS             977..2389
                     /gene="pykF"
                     /locus_tag="OO130_001425"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HCR5364892.1"
                     /translation="MKKTKIVCTIGPKTESEEILAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            2700..2936
                     /gene="lpp"
                     /locus_tag="OO130_001426"
     CDS             2700..2936
                     /gene="lpp"
                     /locus_tag="OO130_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HCR5364893.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(3000..4004)
                     /gene="ldtE"
                     /locus_tag="OO130_001427"
     CDS             complement(3000..4004)
                     /gene="ldtE"
                     /locus_tag="OO130_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HCR5364894.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLLPVVPAGPNNPLGRYALRFAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRARQ
                     "
     gene            complement(4153..4569)
                     /gene="sufE"
                     /locus_tag="OO130_001428"
     CDS             complement(4153..4569)
                     /gene="sufE"
                     /locus_tag="OO130_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HCR5364895.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRDEDRSP
                     QNSIQGCQNQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            complement(4582..5802)
                     /gene="sufS"
                     /locus_tag="OO130_001429"
     CDS             complement(4582..5802)
                     /gene="sufS"
                     /locus_tag="OO130_001429"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HCR5364896.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLHTL
                     FDEKTRLLAITHVSNVLGTENPLAGMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSVLGLNNIAEYELNLMHYALSQLESVPNLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            complement(5799..7070)
                     /gene="sufD"
                     /locus_tag="OO130_001430"
     CDS             complement(5799..7070)
                     /gene="sufD"
                     /locus_tag="OO130_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HCR5364897.1"
                     /translation="MAGLPNSSNALQQWHHLFDAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTSLEGLTNSQFVSIAGEISPQQRDALALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMYITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPLSHHFAHNDLLLAEDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            complement(7045..7791)
                     /gene="sufC"
                     /locus_tag="OO130_001431"
     CDS             complement(7045..7791)
                     /gene="sufC"
                     /locus_tag="OO130_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HCR5364898.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLAVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            complement(7801..9288)
                     /gene="sufB"
                     /locus_tag="OO130_001432"
     CDS             complement(7801..9288)
                     /gene="sufB"
                     /locus_tag="OO130_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HCR5364899.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            complement(9297..9665)
                     /gene="sufA"
                     /locus_tag="OO130_001433"
     CDS             complement(9297..9665)
                     /gene="sufA"
                     /locus_tag="OO130_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HCR5364900.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            complement(10212..10400)
                     /locus_tag="OO130_001434"
     CDS             complement(10212..10400)
                     /locus_tag="OO130_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HCR5364901.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            complement(10500..10910)
                     /gene="menI"
                     /locus_tag="OO130_001435"
     CDS             complement(10500..10910)
                     /gene="menI"
                     /locus_tag="OO130_001435"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HCR5364902.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            complement(10907..13963)
                     /locus_tag="OO130_001436"
     CDS             complement(10907..13963)
                     /locus_tag="OO130_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCR5364903.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDVLDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDQEMLGLNIVEFAGDDEALIDERVNALCARLDELIASHQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPELDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTL
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLESLNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESQPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            14352..15464
                     /gene="ydiK"
                     /locus_tag="OO130_001437"
     CDS             14352..15464
                     /gene="ydiK"
                     /locus_tag="OO130_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HCR5364904.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            15650..15757
                     /gene="rprA"
                     /locus_tag="OO130_001438"
     ncRNA           15650..15757
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="OO130_001438"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            15893..16272
                     /locus_tag="OO130_001439"
                     /pseudo
     CDS             15893..16272
                     /locus_tag="OO130_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001014786.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
     gene            16217..16939
                     /locus_tag="OO130_001440"
                     /pseudo
     CDS             16217..16939
                     /locus_tag="OO130_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            16951..17817
                     /gene="ydiB"
                     /locus_tag="OO130_001441"
                     /pseudo
     CDS             16951..17817
                     /gene="ydiB"
                     /locus_tag="OO130_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
     gene            17849..18607
                     /gene="aroD"
                     /locus_tag="OO130_001442"
     CDS             17849..18607
                     /gene="aroD"
                     /locus_tag="OO130_001442"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HCR5364905.1"
                     /translation="MKTVTVKDLVIGTGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVACAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            18753..20348
                     /locus_tag="OO130_001443"
     CDS             18753..20348
                     /locus_tag="OO130_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HCR5364906.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SELAEQNKIIAYNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKE
                     DLIKLVEFDNKEYLYYKAIAPDIAFIRATTCDSEGYATFEDEVMYLDALVIAQAVHNN
                     GGIVMMQVQKMVKKATLHPKSVRIPGYLVDIVVVDPDQSQLYGGAPVNRFISGDFTLD
                     DSTKLSLPLNQRKLVARRALFEMRKGAVGNVGVGIADGIGLVAREEGCADDFILTVET
                     GPIGGITSQGIAFGANVNTRAILDMTSQFNFYHGGGLDVCYLSFAEVDQHGNVGVHKF
                     NGKIMGTGGFIDISATSKKIIFCGTLTAGSLKTEIADGKLNIVQEGRVKKFIQELPEI
                     TFSGKIALERELDVRYITERAVFTLKEDGLHLIEIAPGVDLQKDILDKMDFTPVISPE
                     LKLMDERLFIDAAMGFVLPEAAH"
     gene            20362..21513
                     /locus_tag="OO130_001444"
     CDS             20362..21513
                     /locus_tag="OO130_001444"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HCR5364907.1"
                     /translation="MDFSLTEEQELLLASIRELITTNFAEEYFRTCDQNGTYPREFMR
                     ALADNGISMLGVPEEFGGIPADYVTQMLALMEVSKCGAPAFLITNGQCIHSMRRFGSA
                     EQLRKTAESTLETGDPAYALALTEPGAGSDNNSATTTYTRKNGKVYINGQKTFITGAK
                     EYPYMLVLARDPQPKDPKKAFTLWWVDSSKPGIKINPLHKIGWHMLSTCEVYLDNVEV
                     EESDMVGEEGMGFLNVMYNFEMERLINAARSTGFAECAFEDAARYANQRIAFGKPIGH
                     NQMIQEKLALMAIKIDNMRNMVLKVAWQADQHQSLRTSAALAKLYCARTAMEVIDDAI
                     QIMGGLGYTDEARVSRFWRDVRCERIGGGTDEIMIYVAGRQILKDYQNK"
     gene            complement(21556..22467)
                     /locus_tag="OO130_001445"
     CDS             complement(21556..22467)
                     /locus_tag="OO130_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCR5364908.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAFAYTLAIL
                     YYENFKNAYRSEQGYIKKDVLIKDILFYLSNNYREKITLEQLSKKFRASVSYICHEFT
                     KEYRISPINYVIQRRMTEAKWSLTNTELSQAEISWRVGYENVDHFAKLFLRHVGCSPS
                     DYRRQFKNCFAEQEILSEFPQPVSLVG"
     gene            22781..23552
                     /locus_tag="OO130_001446"
                     /pseudo
     CDS             22781..23552
                     /locus_tag="OO130_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
     gene            23572..24510
                     /locus_tag="OO130_001447"
     CDS             23572..24510
                     /locus_tag="OO130_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HCR5364909.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAAPLKDKHPAMLLLVATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGAKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            24566..25855
                     /locus_tag="OO130_001448"
     CDS             24566..25855
                     /locus_tag="OO130_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR5364910.1"
                     /translation="MSDDKFDAIVVGADVASSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILQANVVVLAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQMVNDGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KEREDFSASSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYSRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            25852..26145
                     /locus_tag="OO130_001449"
     CDS             25852..26145
                     /locus_tag="OO130_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HCR5364911.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILAANPDINEFRKLMKA
                     CPAGLYKQDDAGNIHFDSAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            26202..>27676
                     /gene="fadK"
                     /locus_tag="OO130_001450"
     CDS             26202..>27676
                     /gene="fadK"
                     /locus_tag="OO130_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HCR5364912.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGATYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWREAELVSVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSPIIADNTSLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWQDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNVLEKQPADLSALRFFLCGGTTIPKKVARECQQRGIKLLSVY
                     GSTESSPHAVVNLDDPLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYCSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSL"
BASE COUNT         6907 a         7001 c         6803 g         6965 t
ORIGIN      
        1 aactgatatt tattatcatt tgaaataaat ttaacttaag cgacaattat ttgtgacgtt
       61 cattgcagaa aataaaaata atattatcgt tataaattaa aatacatacc gcaattcgta
      121 taatttcagc agttgttatt tagaatgatt aactggaccg caactgaagc aagaatatca
      181 ggatgaagct taccgcctca tcctgaatgg tcattttacc agtgcgttaa tgacggtttc
      241 catcgcctgc ttatccagct cactgcgctg aatgcgttta ttggctaatg ctgtacgtaa
      301 tacgcctgcg ataacaatat gctttggttc ctgccccaga tcgcgcattt ccagcacaac
      361 tttaccgaca actctgcaca tttctcggta cagttcatcc tcttttgtcc gatttcccat
      421 tctttatacc tatttatcat gccaactatc agcatatatc aatctaacca attaaacaaa
      481 aatcaaacaa aatcagacaa ataacgcgat aaattatttt aactgtagca attgagcgat
      541 gatatattta tacaccggat gaactttcac ttatcctcac actgacaact ttggcaccag
      601 acgttgcgca aacagcgaag tttttgcgta accttttccc tggaacgtta aatctttgat
      661 aacaatttat tgtctaacaa gttgtatatt ttttgaaacg ctgtttttgt tttccttttg
      721 gattaatttc agcgtataat gcgcgccaat tgactcttga atggtttcag cactttggac
      781 tgtagaactc aacgactcaa aaacaggcac tcacgttggg ctgagacaca agcacacatt
      841 cctctgcacg ctttttcgat gtcacctatc cttagagcga ggcaccacca ctttcgtaat
      901 accggattcg ctttccggca gtgcgcccag aaagcaagtt tctcccatcc ttctcaactt
      961 aaagactaag actgtcatga aaaagaccaa aattgtttgc accatcggac cgaaaaccga
     1021 atctgaagag atattagcta aaatgctgga cgctggcatg aacgttatgc gtctgaactt
     1081 ctctcatggt gactatgcag aacacggtca gcgcattcag aatctgcgca acgtgatgag
     1141 caaaactggt aaaaccgccg ctatcctgct tgataccaaa ggtccggaaa tccgcaccat
     1201 gaaactggaa ggcggtaacg acgtttctct gaaagctggt cagaccttta ctttcaccac
     1261 tgataaatct gttatcggca acagcgaaat ggttgcggta acgtatgaag gtttcactac
     1321 tgacctgtca gttggcaaca ccgtactggt tgacgatggt ctgatcggta tggaagttac
     1381 cgccattgaa ggtaacaaag ttatctgtaa agtgctgaac aacggtgacc tgggcgaaaa
     1441 caaaggtgtg aacctgcctg gcgtttccat tgctctgcca gcactggctg aaaaagacaa
     1501 acaggacctg atctttggtt gcgaacaagg cgtagacttt gttgctgctt cctttattcg
     1561 taagcgttct gacgttatcg aaatccgtga gcacctgaaa gcgcacggcg gcgaaaacat
     1621 ccacatcatc tccaaaatcg aaaaccagga aggcctcaac aacttcgacg aaatcctcga
     1681 agcctctgac ggcatcatgg ttgcgcgtgg cgacctgggt gtagaaatcc cggtagaaga
     1741 agttatcttc gcccagaaga tgatgatcga aaaatgtatc cgtgcacgta aagtcgttat
     1801 cactgcgacc cagatgctgg attccatgat caaaaaccca cgcccgactc gcgcagaagc
     1861 cggtgacgtt gcaaacgcca tcctcgacgg tactgatgca gtgatgctgt ctggtgaatc
     1921 cgcaaaaggt aaatacccgc tggaagcggt ttctatcatg gcgaccatct gcgaacgtac
     1981 cgaccgcgtg atgaacagcc gtctcgagtt caacaatgac aaccgtaaac tgcgcattac
     2041 cgaagcggta tgccgtggtg ccgttgaaac tgctgaaaaa ctggatgctc cgctgatcgt
     2101 ggttgctact cagggcggta aatctgctcg cgcagtacgt aaatacttcc cggatgccac
     2161 catcctggca ctgaccacca acgaaaaaac ggctcatcag ttggtactga gcaaaggcgt
     2221 tgtgccgcag ctggttaaag agatcacttc tactgatgat ttctaccgtc tgggtaaaga
     2281 actggctctg cagagcggtc tggcacacaa aggtgacgtt gtagttatgg tttctggtgc
     2341 actggtaccg agcggcacta ctaacaccgc atctgttcac gtcctgtaat attgcttttg
     2401 tgaattaatt tgtatatcga agcgccctga tgggcgcttt ttttatttaa tcgataacca
     2461 gaagcaataa aaaatcaaat cggatttcac tatataatct cactttatct aagatgaatc
     2521 cgatggaagc atcctgtttt ctctcaattt ttttatctaa aacccagcgt tcgatgcttc
     2581 tttgagcgaa cgatcaaaaa taagtgcctt cccatcaaaa aaatattctc aacataaaaa
     2641 actttgtgta atacttgtaa cgctacatgg agattaactc aatctagagg gtattaataa
     2701 tgaaagctac taaactggta ctgggcgcgg taatcctggg ttctactctg ctggcaggtt
     2761 gctccagcaa cgctaaaatc gatcagctgt cttctgacgt tcagactctg aacgctaaag
     2821 ttgaccagct gagcaacgac gtgaacgcaa tgcgttccga cgttcaggct gctaaagatg
     2881 acgcagctcg tgctaaccag cgtctggaca acatggctac taaataccgc aagtaatagt
     2941 acctgtgaag tgaaaaatgg cgcacattgt gcgccatttt ttttgtctgc tatttaccac
     3001 tactgcctcg cgcgtaacat attcccttgc tctggttcac cattctgcgc tgactctact
     3061 gaaggcgcat tgctggctgc gggagttgct ccactgctca ccgaaaccgg ataccctgcc
     3121 cgacgataca acgctttatc gactaacttc tgatctacag ccttattgtc tttaaattgc
     3181 gtaaagcctg ctggcagtgt gtatggcatt gtctgaacgt tctgctgttc ttctgccgat
     3241 agtggtcgat gtacttcaac ataacgcatc ccgttaggct ccacggaata tttcaccggt
     3301 tcgttgatca ctttcaccgg cgttcccgtc cgcacgctgg agaataaggc tttaatatcc
     3361 ggtgcattca tgcgaataca gcccgaactg acgcgtaaac cgacgctgtc cggcgcactg
     3421 gtaccatgaa tgaggtattc gccattacca tgcgcgaagc gcagtgcgta acgtcctagc
     3481 gggttatttg gtccggcagg aacgactggc agtaatttaa tgccacgctc cagcgaacgt
     3541 tgacgaatgc ctgccgtagg cgtccaggtt ggattaggga ttttctgccc cacacgcgtt
     3601 tccatcaccg gcgtttccag cccctgcaag ccaataccta tcggataaac ctgcacgata
     3661 ttttctcccg gcggataata ataaaggcgc agctctgcaa ggttaacgat aatcccctga
     3721 cgcggtgcat caggtaataa cagttgtgaa ggaatagtta tcgtcgtacc aggttttggc
     3781 accggggcga tagtgttatt ggcttcaagg atcaacattg ccgcagtatc aaaacgtcgg
     3841 gcaatagcct gaaggttttt atccccttct tgcaccgtat acgtttgatt ttgcccaacc
     3901 agtcggcttc cggttggtgg tagcggataa tcaaccgccc aggcagcctg gatggcgcta
     3961 aaagcgccga taagcgtgag tgtaagcaaa gacgcgcgtt tcattgtaaa cctcctgtat
     4021 ttgccggaga ctcacgctga aacgtcggat ggcgcttatg ttcacctgaa accaaaacac
     4081 tcctgtgcag gtcagtgtaa acattgacca tccggcaatg tgagccaacc ggatgaaagc
     4141 tgtcccttta gtttagctaa gtgcagcggc tttggcgcga attgcgcgaa tcatcgcttc
     4201 cagaccttgt gaacgagatg gggtgagatg ttgggtgagc gccatttttt caaaccacgg
     4261 acgcacatcg aaattgacaa tatcctgcgg cgtcatctga tcgtagagaa taaatacgac
     4321 cgcaataagc cctttcacaa tcgccgcatc gctgtcgccc tgcaattcaa taattccctg
     4381 cgcattctgg cgcatgacaa tccacacctg attctggcag ccctgaatgc tattttgtgg
     4441 gcttcggtct tcgtcgcgta attctggcag acgctggccc agctcaataa tgtagagata
     4501 tttctcttcc cagttggcgc agcgtaaaaa attacgcagc aatttttctt tatccggcaa
     4561 taaagccata gtgcctccct gttatcccag cagacggtga atacgttgca ggccggtcac
     4621 cagacgatcc acttcttcat gagtgttata catggccagc gacgcccgac acatcgcagg
     4681 gacgttgtaa taggccatta atggcatagc gcagtgatgt ccggtacgca cagcaatgcc
     4741 gtaattatcg agaaaactgc caacatcata ggcgtggtgt ttaccgagat taaaagcaat
     4801 aacgccaagc ctgttttgtg ggccatagag agtgagattc ggtacagatt ccagctgtga
     4861 tagcgcgtaa tgcatcagat tcagttcata ctcggctatg ttattaagcc ccagcaccga
     4921 aacatattcc agcgccgcgc caagaccaat gatgcccccg gtattgggtg taccggcttc
     4981 aaaccgccat ggtgctttgg tccaggtagt gccttcactc aggctgacgg tggcgatcat
     5041 agaaccgccc ccttcccacg gcggcatctc ctgcaacaag gcttctttga cataaagaat
     5101 gccaatcccg gtggggccat acagtttatg cccggaaaac acgtaaaaat cgcaatccag
     5161 cgcctgaaca tccaccggat gatgcatcac cgcctgagcg ccatccacca gcacttttgc
     5221 gccatgctgg tgcgcaagcg tgatcattcc cgccagtgga ttttctgtgc caagcacgtt
     5281 ggagacatga gtaattgcca gcaggcgagt tttctcatca aacagcgtat gcagcgtctc
     5341 cagttgcagc gtaccgtcgg ggttgagagg gatcacacgc agctctgcgc caacgcgtgc
     5401 gcaaagcatc tgccagggga caatgttagc gtggtgctcc atctgactga tgatgatgtt
     5461 atcgcccgcc cgcacattgc tgttgcccca gctattggcg accagattga tcccttccgt
     5521 cgtgccgcgg acgaacacca gctcttccgc cgaacgggca ttaataaaca gcgatgcccg
     5581 cttgcgcacg ttctccattt tctcggtcgc ctgggcgctt aaggtatgaa taccgcgatg
     5641 caccgccgcg tagccatgac gataaaactc ggcctcggcg tcaatcacct ggctcggttt
     5701 ctgtgcactg gcggcgctgt cgagataagc cagcggcaaa ccgtttacct cacgcgaaag
     5761 caccggaaag tcggcccgca ctttgtcgac ggaaaaagtc atcttgcacc tcctggcagc
     5821 cgttgaccga ttcgggccag cacctgctgt ttaagcccct catcacgcag tgcttccgtc
     5881 agttcggcag caaaggcgta aatgatcatc tgctgggcat cctgctgatt gatcccgcgc
     5941 gagcgcagat agaacatctg ttcatcatca atacgcccca ccgtcgcgcc gtggctgcat
     6001 ttcacatcat ctgcatagat ttccagctgc ggtttcgtat ccacttccgc cagtttgccc
     6061 atcagcagat tattgttggt catctgacca tccgttttga tggcgtgctg cgcgacgttg
     6121 atcaaaccgt taaataccgc gcggcctttg tcgctgacga tagttttgtg caactgtcgg
     6181 ctgttacaaa aacctttatt gtgttccagc caggtacggg tatcacacac ctcgtttttc
     6241 accggcatcg ccaggctatt gatccgcagc gtgctgtttt cgccattgag ttgcgtactg
     6301 gtgttgtgtc gtaacactgc gccacccagc aggaaactat ggctaaatgc ggtggcatcc
     6361 tcagccagca acaaatcgtt atgagcaaag tggtgactga gcgggttttc aaacgccagc
     6421 ttgatatgct gcaagtgggc gttcgctgcg acattgatag tgaaccgtgc cccggtaaaa
     6481 tgacgagcat cattaaggct gacaaaatgt tcgatcaccg ttgcttcggc accttccgcc
     6541 agatccagat gatgccggta atgggcagtg ttcacctctt cacctgccac gccctgggtg
     6601 atatacatta acagcaatgg ctttgccggc cgttgaccgc gcttcacggc gatatgcgtc
     6661 acgctttgtg ccaggctttc cgtcaaatgc agaaacactt ccgcctgaat agcgtcgggt
     6721 aacccctgac ggtcgtcgtt aatgctcact tcatatccgc tgccttcaat tgcatcgctc
     6781 agcgcgggca cgtaacgtcc atcgacaaac accagccgca cggcgtctaa cgttaacgct
     6841 aaggcatcac gctgctgtgg ggatatctcc cccgcaatgc tgacaaactg gctattggtc
     6901 agcccttcca gcgacgtata tttccagttt tcatgtttac gtgtcggcag tccggtacgc
     6961 agcaattgtt gtaaatgctg ctgtgcttgc ggggagcgtt ttgtcccttc agcgtcaaac
     7021 aagtgatgcc actgttgcag cgcgttactg ctgttcggta agccagccat aaccctgctc
     7081 ctccagttgt ttgaccaacg tgaaatcgcc ggatttcaca attcgtccct gatatagcac
     7141 atggacgtaa tcaggcttga tgtagtcgag aatgcgttgg tagtgcgtaa caatgatgaa
     7201 tgagcgcttg ccatcacgca gggagttcac gccatcggcg accactttta atgcgtcaat
     7261 atccagcccg gagtccgact catcaagaat gcataactcc ggttccagca ccgccatttg
     7321 caaaatatcg ttgcgctttt tctcgccgcc ggaaaaacca acgtttaccg aacgggttaa
     7381 taaatcttcc ggcatcttca ggagagcgat tttctcttcc atcaaatcct gaaaatcaaa
     7441 gcggtcgagc gtttcctggc cgcgatagct gcgcactgca ttaagtgccg tttgcaggaa
     7501 aaactggtta ctgacgcctg gaatctccac cggatactgg aaggccataa agatgccttc
     7561 gcctgcacga tcttccggcg acagtgcaag caaatctttg cctttgaact caaccgtgcc
     7621 gcccgtcact tcataatctt cgcgcccggc aagcgttgcc gataaggtac ttttgcccga
     7681 accgtttggc cccataatgg cgtgaacttc gccgggacga acggcgaggc ttaatccacg
     7741 caggatagct ttatcttcca cgctgacgtg taaatcttta atacttaaca tgtttattcc
     7801 ttatccgacg ctgtgttcaa gactgatcgc gaggagtttt tgtgcttcaa cggcaaattc
     7861 cagcggcagt tctgagaaca cgtctttgca gaaaccgtta acaatcatcg agatggcgtc
     7921 ttcttcgctg atcccgcgtt gcaggcagta aaacagttga tcttcaccaa tacgtgatgt
     7981 cgttgcctcg tgctccagtt gtgcgctgtt attgcgacat tcgacatacg ggaaggtatg
     8041 cgccccacaa ttagcgccaa tcagcattga gtcgcactga gtgaaattgc gcgcattggt
     8101 tgccgtcggc atgattttca ctaagccgcg ataactgttc tgactatgtc cggcagagat
     8161 ccctttcgag ataatggtcg atttggtgtt tttaccgatg tggatcatct tggtgccggt
     8221 atccgcttgc tgatgaccgc tggtcagcgc cactgagtaa aactcaccaa tggagttatc
     8281 gccgcgcaaa atgcagctgg gatatttcca cgtaatcgcg gacccggttt ctgattgcgt
     8341 ccatgacatt ttgctgtttt cgccttcgca caaagcacgc ttggtgacga agttgagaat
     8401 accgccggtg ttgttatcgc cagggaacca gttttgtacc gtggaatatt tcacctcggc
     8461 gtttttatgg atgatgactt ccaccactgc cgcgtgtaac tgatagctgt cacgcaccgg
     8521 agcggaacag ccttcaatgt agctgacgta gctgtcttcg tcggccacca gaatggtgcg
     8581 ctcaaactgc ccggtttttt ccgcgttaat gcgaaaatag gtggaaagtt ccatcgggca
     8641 gcgcacgcct ttaggcacat aaataaacgt accgtcagag gctaccgccg cattaagcgc
     8701 ggcaaagaag ttgtcattcc ccggcaccac ggtgccgaga tatttacgca ccagttccgg
     8761 gtgatcgtgg atcgcctcac caaaggaaca gaaaataatt ccctgctccg ccagtttttc
     8821 gcgataagta gtggcaaccg aaacagagtc gaaaatggca tccaccgcca cctctttgcc
     8881 ttcccgcacg ggaacgccca actgctcaaa cgccgcctcc acctctttac ttaaaaaggc
     8941 gttcgcgcca gtttgctgca ccgcgccagg ttcagacgcg caagtgtcgt cacaattacc
     9001 gcacgatggt gctgagtagt agctgtaatc ctgataattc agcttgtcgt agtgcgcttt
     9061 caaccagtgc ggttcttcca tctccagcca tgcgcgatag gcgtttagac gaaactccag
     9121 catccactcc ggctcattac gcttcgccga aattgcgcgc accacctctt cgtttatccc
     9181 ctttgccagc tcatcggtgg ctaactgggt gaagaatcct tctttataat tcagcgggcc
     9241 gccggtccag gttttgacat cgtcagttgc ttcagtatta cgagacatag taccgcctat
     9301 accccaaagc tttcgccaca gccacattca ttctgggctt tagggttgtg aaatttgaat
     9361 atctgattaa gtccttcacg aacgaaatcg acttccgtgc catcaataaa cggcatcgct
     9421 tgcagcggga caaacagctt cgcgccgtcg tgttcaaaca gcagatcgtc tttgtccggc
     9481 tcgctaacac tgtcgagcac atagccaaag cccgcgcagc ccgtttgctt cacgcctaaa
     9541 cgcacgccga ccatacccgg ctgctttgcc accagctcac ggatgtgtat cgccgctgcg
     9601 ggtgtcagcg ttaagccttg ccaggcgaaa tcttgtgggt taaaggttcc tgaatgcatg
     9661 tccatcgatt tacctcactt catcgctttc agcgtataac agcatgttgg tgataatgat
     9721 tatcagttca acccagcaaa cgcaggggct ttaccgtaaa acatgctttt tgcctgcttt
     9781 taataagcat agaccctgat gtgtgggtta acaggcacgc taagaattag gtatctcatt
     9841 gttagataat ggttattatc taaggagcat taaggctgtg atgaaaaaga aaaagttgta
     9901 ttaaaatgac tatttaagag ataggtaaaa aagtgcagcg ttcagaaata agaaaaccct
     9961 taagtctgtg cgacacaggc ttaagggttt ctaccccatc cggcgcttat ctccggcact
    10021 ctcagtggct tagctcttga aggggcgata agaataatct cataaagcta acccgccgtt
    10081 ttaacacaaa ctgcgattag tattattttt gaacaatatc aggcggtaga taagcagtat
    10141 taagaaggtc atcgaacctg gacggaggtt aatccaggtc gatttggcga acttgcggca
    10201 ttaagtcagg atcaatgcac gcccagacgc caggcaaagt agatttcttc ttttaattca
    10261 gcagaagaga gagtaagcag gtcagcaaat tcaagttcta gttgtttcag acgtttgaga
    10321 tattgggcag gtgaaagatt gctctggtca cggcgtaaaa attcaatggc cagctgggtg
    10381 ggatcaagtt gagtagacat agcatcctcg cttttagaca agacgtgtac agtataccac
    10441 cgtttactgt gcagataatg accaaaagca atatgcgtca cacttttctg gtgacaacgt
    10501 cacaaaatgg cggtcgtcaa tcgtgacgaa cagcacaaac gccctttctc atcgaagatt
    10561 tcaatctgcc agacctggtg acgcgaaccg agatgcaacg gtttacatat gccgcgcacc
    10621 cgcccttctc gtgccgagcg gacgtgatta gcattgattt ccagaccaac cactttttgc
    10681 tcaccttcgg tacataaata accggcaacg gaaccgatac tttcggccag taccacggat
    10741 gctcctccat gcagcaaccc gaaaggctgc tttgtccgcg agtctactgg cattgtcgct
    10801 tcaagggtgt catcaccaat atgttcaaag cgaatatcca ggaaccccac catgtttcct
    10861 tcacccatag cattcagtgc ttccagggtg attttccgtt tccatatcat ttaataatct
    10921 ccagtaaagc ctgcacagga tggcgtaccc ccgtgccttc aacccgtttt acctggctac
    10981 ggcaggaata tccggtcgcc agacagcggt tacgcggcag tcgctgcata gcctgatgcc
    11041 aggataactc atagatcccg agcgaatttt catggttttt cgcttcatgt ccgtaagtcc
    11101 ctgccatgcc gcagcaaccc acgctgacat tttccagttt cgcgccaaaa cgggcaaata
    11161 tcgcggccca ttgtgctggc gcacccggca aggcggtaac ttcggtacag tgaccaaaga
    11221 aataccatga ttcaccgctg actgtagcca caggctgtga ctcaagtgcg cttgccagcc
    11281 attcattcgc cagtaagacg ttaaactcgc cacgctcctc gcccagcgcc agtttatatt
    11341 catcgcgata acaaagtacc agcgccggat cgacgcccac cattggcata cccagcttcg
    11401 ccatacggtt gaggaaatcc gccgtctttt tcgccgtctt cgcaaaacga ttaagaaaac
    11461 ctttaatatg ctgggctttg ccatttggcg aaaatggcag taacacaggc tggaaaccta
    11521 atttttcgac cagacggaca aaatccgcca ccacttgcgc atcgtaatag ctggtaaagg
    11581 ggtcctgcac caccaacact gtgcgcgctt tctgctctgc attgagagat tcaagctgtt
    11641 ccagcgtcat gtttgccgag cgatgcccca ccatttgttg ttgtagcgag gggaccgaca
    11701 gcagcggcaa atcaaccatg ccgatatgtt tttccgagag tttgcgcacc agcggctggt
    11761 taatgaagaa gttaaaggtt ttcggcgcgc gtgccatcag cggcgcgtag ctctcgaccg
    11821 tagcgacgag gtggtcgcgc agcgggcgta aataacgggt gtgatagagc tgcagaaaac
    11881 gagagcgaaa ctccggcaca tcaattttga tggggcacag ggtcgaacac gctttacagg
    11941 ccagacagcc cgacatcgcc tctttgactt cgtgtgagaa gtcatattcg cctttattcg
    12001 catgccagct attgcgcgtg cgggcaatta acgtccgcaa actgacgccg gattcaggca
    12061 gttcttgttc cagtttgagt ggatcaacgc cgcgatccgc caacaaacgc agccattcac
    12121 gcaccagcgt tgcgcgccct ttcggtgaat gaatccggtt ctgggtgatc ttcatcgacg
    12181 gacacatcgg actacgggca tcaaagttga agcataaacc gttgccgtta cactccatcg
    12241 caccgcgcca ctgctggcgt accgcaatgg ggatctgccg atcgaatgta ccgcgcttca
    12301 ccgcgtccac tttcatcatc ggcgcatcga gaccttctgg cgggcaaatc ttccctgggt
    12361 tgagtcggtt atgcgggtca aatgccgctt tcactttgcg cagttctgca aaaagttcct
    12421 caccgaaaaa cgccgggctg tattcagcgc gaaaaccttt gccgtgctcg ccccacaaca
    12481 aaccaccgta tttcgcagtc agcgccacca cgtcatcaga gatttgcttc atcaaaatct
    12541 cttgttgagg atcgcacata tccagctctg gacggacgtg caagacacct gcgtcgacgt
    12601 gaccgaacat accgtagctt aagccgtggc tgtcgagcag cgcgcgaaat tcagcaatat
    12661 aatccgccag gtgttccggc ggtacgcagg tatcctcagc aaacggaatt ggcttagcgg
    12721 cacctttggc attgccaagc agaccaacgg cttttttgcg cattgcatag atacgttcaa
    12781 cccccgccag ctcacggcac acctgccagc cgatgacacc tgcttgatga ctggcgatca
    12841 gctcatccaa ccgcgcacag agtgcattta cccgctcatc aatcagcgcc tcatcatcac
    12901 cagcaaattc cacaatgttc agcccgagca tctcttggtc aggcacatcg gtaatcaact
    12961 cgctgacgga atgccagaca atatcttccc gcgccagatt cagcactttt gagtccaccg
    13021 tctctaccga aagcgcccgc gcctcaacca taaacggcgc gttacgcagc gcagagtcaa
    13081 aagagtcata tttgacgttc accagacggc gcactttagg caagcgcgta atatccagcc
    13141 gcgcttcggt aataaaggcc agcgtccctt ctgaacccgt cagaatgcgc gtcaggtcga
    13201 actcggtcat ctcatcgtta aagacatgac gcagatcgta accggtaaga aagcggttaa
    13261 gtttggggaa gttgtcgata attaactggc gttgctgacg gcaacgttga taaaccgtgt
    13321 tataaattcg cccgattgtg gtattggatt tacccagcgt ttccgccaat tcgacgggta
    13381 aaggttgcgt atcgagaaca tcgcccccca acaacaccgc gcgtacgcca agtacgtgat
    13441 ctgacgtttt gccatagacc agcgatccct gaccggatgc atcggtattg atcatcccac
    13501 cgagcgttgc ccggttgctg gtcgaaagtt ccggcgcaaa aaagtagccg aacggtttca
    13561 ggtactgatt gagttgatct tttatcaccc cggcctcaac gcgcacccag ccctcttcag
    13621 ggttaatttc gatgatgcgg ttcatatggc gggacatatc aacaataatc ccctggttga
    13681 gcgcctgacc gttagtgccg gtgccgccgc cgcggggggt aaagatcagc gatgaatagc
    13741 gttcctgcgc ggcaagacgg gcgatcagcg ccacatctgc ggttgaacgc ggaaatacca
    13801 ccgcatcggg gagaagttgg taaatactgt tgtcggtcga cattgtcaga cgatcggcat
    13861 aacttgtcgc cgtatcgccg gtaaaacctt gttgctccag ctcttgcaaa aaattaagca
    13921 ccagttgaac gacgccgggt gcctgagaaa tctgtggaat cattatattg cccctttcct
    13981 gcggtctgtg atgtaggtcg atacactatt ctttcaggcg gctgcaatag cgcactgaaa
    14041 ggtgatgttt atttactcta tggatttcga gttgcaggaa ggcggcaagc gagtgaactc
    14101 caggagctta caatagtaag tgactggggt gaacgaacgt agccgcagca catgcaactt
    14161 gaaatacgac gagtaaatcg tttgcgtgtt gcctgagttg ttgtaccaca tttttttcta
    14221 acacgcccat cagaattaag ggcagaatcg gcctgttaaa aaccgctgaa attgctcatc
    14281 attatgcagg tgagtttcgc gtgttcacgt cgcgtcgacg atttgacgca caaaaaaggt
    14341 gaaaagtagt tatggtaaat gttcgtcagc ccagggatgt cgcacaaatt ctgctttcgg
    14401 tgctgttttt agccatcatg attgtggcat gtctgtggat tgttcaaccc tttattctcg
    14461 gctttgcatg ggccggtacg gtggttatcg ccacctggcc ggtattgtta cgtttgcaaa
    14521 agatcatgtt tggccgccgc tctctcgccg ttctggtgat gacgctgtta ttagtgatgg
    14581 tgtttatcat ccctattgct ttgctggtta acagtatcgt cgacggcagc ggcccgctaa
    14641 ttaaagccat ttccagcggc gacatgacgt tacccgatct ggcgtggctt aataccattc
    14701 cggtgattgg cgcgaagctg tatgcaggct ggcacaactt gctggatatg ggggggacgg
    14761 cgatcatggc gaaagtccgc ccttatattg gcaccaccac cacctggttc gttgggcagg
    14821 cggcgcatat cgggcgcttt atggtgcatt gtgcgctgat gcttctcttc agtgccctgc
    14881 tgtactggcg cggtgaacag gtggcacaag gcattcgcca ttttgcaacc cgtctggcag
    14941 gcgttcgcgg agatgccgcc gtactgctgg cggcacaggc tatccgcgcg gtggcgctgg
    15001 gtgtggtggt gacggcgtta gtacaggcag tgcttggcgg tatcggcctc gccgtatccg
    15061 gcgtacctta tgcaacgttg ctaacggtgt taatgatcct ctcctgcctt gtccagcttg
    15121 gcccgttgcc ggtactgatt ccggcgatta tctggctcta ctggactggc gataccacct
    15181 ggggaacggt attgttagtg tggagcggtg tggttggcac gctggataac gtcatccgcc
    15241 caatgttaat tcgcatgggt gccgatttac cgctgatcct gatcctctct ggtgttattg
    15301 gtggtttgat tgctttcggg atgatcggtc tgtttattgg tccggttctg ttagccgttt
    15361 cctggcgtct gtttgccgcg tgggtggaag aagtcccgcc gccgacggac caaccggaag
    15421 aaattctcga agaacttggc gaaatcgaga aaccgaataa gtaatttctc atcaggcggc
    15481 tctgccgcct gattgttaac ccccgctaat tgatatttgc ttgctcttcc ccatattttc
    15541 ctgcttaccg ccattcagct ggtagtacct gtcgcaaatt ctttacagtt tttaaactaa
    15601 tgagacgaat ctgatcgacg caaaaagtcc gtatgcctac tattagctca cggttataaa
    15661 tcaacatatt gatttataag catggaaatc ccctgagtga aacaacgaat tgctgtgtgt
    15721 agtctttgcc catctcccac gatgggcttt tttttaacat ttttccactt cgctacctcg
    15781 cccctcactc tttccaataa tgttgcacaa gatactgtga ctctcttcaa gccgtcagca
    15841 ggctgataat gttatttata ttttattgtt tatttcatgc agccattaca cgatgaacgc
    15901 ttatgaactc caggcactcc gacatatttt tgccatgact attgatgaat gcgcacctgg
    15961 attgcccaga ccggtgacag tgaaagctgg cgtcagtggg aaaatggcaa gtgtgccatt
    16021 cctgatcgtg tagtcgagca actgttggct atgcgtcagc aaagaaaaaa acatcttcat
    16081 gccatcatag agaaaataaa taaccgcatc ggcaacaaca ccatgcgttt tttccccgac
    16141 ttaaccgcat ttcaacgagt ctaccctgat ggcaatttta ttgactggaa gatctatcaa
    16201 tcggtagccg ctgaaggtaa tgctgttgaa aagcaaattc cccagccagt tggtggacgc
    16261 cagcgtagct aatgaattac cgcaaatgaa cagcaaaccg ttagtctggc tggaaggtgt
    16321 ttcatcggta ctgttcggtg tagccgcatt ctcgaccttt tatgtgattg tggtgtggat
    16381 gcccaaatat gcgatggctt ttgctggtat gtcagaagct gaggcattaa aaaccatctc
    16441 ttattacagt atgggctcgt tggtctgtgt ctttattttt gccgcactac tgaaaaaaat
    16501 ggtccggccc atctgggcta atgtatttaa ctctgcactg gcaacaataa cagcagccat
    16561 tatctatctg tacccttctc cactggtgtg taatgccggc gcctttgtta tcggtttctc
    16621 agcagctggc ggcattttac agctcggtgt ttcggtcatg tcagagtttt tcccaaaaag
    16681 caaagccaaa gtcaccagta tttatatgat gatgggtgga ctggctaact ttgttattcc
    16741 gctgattacc ggttatctgt cgaacatcgg ccttcaatat atcattgttc tcgattttac
    16801 tttcgcgctg ctggccctga ttaccgcaat tattgttttt atccgctatt accgcgtttt
    16861 tattattcct gagaatgatg tgcggtttgg cgagcgtaaa tttagcaccc ggttaaacac
    16921 aattaagcat agaggttaaa ggagttaatt atggatgtta ccgcaaaata cgaattgatt
    16981 gggctgatgg cctatcctat ccgccacagt ttatcgcctg aaatgcagaa taaagcctta
    17041 gaaaaagcgg gattgccatt tacctatatg gcctttgaag tggataacga tagttttccc
    17101 ggagcaattg aaggattaaa agctctaaaa atgcgcggaa ctggtgtatc aatgccgaac
    17161 aaacaactgg cttgttaata tgttgatgaa ttaacgccag cagccaaact ggtgggtgcc
    17221 atcaacacca tcgttaatga tgatggctac ctgcgcggct ataacaccga cggcacgggc
    17281 catattcgcg ccattaaaga gagcggtttt gatatcaaag gcaaaacaat ggtgctgtta
    17341 ggggccggag gtgcctcaac ggctattggc gcgcaggggg caattgaagg tttaaaagaa
    17401 attaaactct ttaaccgtcg ggatgagttc ttcgataaag ccctcgcctt cgcgcagcgg
    17461 gttaacgaaa acaccgattg tgtcgtcacg gttaccgatc tcgccgatca gcaagccttt
    17521 gctgaagccc tggcttccgc cgacatttta accaatggca caaaagtggg tatgaaaccc
    17581 cttgagaatg aatcattagt taatgatatc agtctgttac atccgggact tctggtcact
    17641 gaatgcgtgt ataacccgca tatgacgaag ttattgcagc aggcgcaaca agctggttgc
    17701 aaaacgattg atggatacgg catgttgttg tggcaagggg ctgaacagtt cacgttatgg
    17761 actggcaaag atttccctct ggaatatgtt aaacaggtca tggggttcgg tgcctgacag
    17821 gctgaccgcg tgcagaaagg gtaaaaaaat gaaaaccgta actgtaaaag atctcgtcat
    17881 tggtacgggc gcacctaaaa tcatcgtctc gctgatggcg aaagatatcg cccgcgtgaa
    17941 atccgaagct ctcgcctatc gtgaagcgga ctttgatatt ctggaatggc gtgtggacca
    18001 ctttgccgac ctctccaatg tggagtctgt catggcggcg gcaaaaattc tccgcgaaac
    18061 catgccagaa aaaccgctgc tgtttacctt ccgcagtgcc aaagaaggcg gcgagcaggc
    18121 gatttccacc gaggcttata ttgctctcaa tcgtgcagcc atcgacagcg gcctggttga
    18181 tatgatagat ctggagttat ttaccggcga tgatcaggtt aaagaaaccg tcgcctgcgc
    18241 ccacgcgcat gatgtgaaag ttgtcatgtc caaccatgac ttccataaaa cgccggaagc
    18301 cgaagaaatc attgcccgtc tgcgcaaaat gcagtccttc gacgccgata ttcctaagat
    18361 tgcgctgatg ccgcaaagta ccagcgatgt gctgacgttg cttgccgcga ccctggagat
    18421 gcaggagcag tatgccgatc gtccaatcat cacaatgtca atggcaaaaa ctggcgtaat
    18481 ttctcgtctg gctggtgaag tatttggctc ggcggcaact tttggtgcgg taaaaaaagc
    18541 ctctgcgcca gggcaaatct cggtaaatga tttacgcaca gtattaacta ttttacatca
    18601 ggcataagca ataatatttc ggcgggaata tcctcccccg ccgaactaaa aaaaatatat
    18661 tcaaccgtat ttaataaaaa tatttcgtga gtctctgtgc gctaaatatc catctggcgt
    18721 agggaaaatc acacctgaat cagaaattaa caatgaaacc tgtaaaacca cctcgtatta
    18781 atggacgagt gccggtcctg tcggcacagg aagcggtgaa ttatattccc gacgaagcaa
    18841 cactttgtgt gttaggcgct ggcggcggta ttctggaagc cacgacgtta attactgctc
    18901 ttgctgataa atataaacag actcaaacgc cacgtaattt atcgattatt agtccaacag
    18961 ggcttggcga tcgcgccgac cgtggtatta gtccgctggc gcaagaaggt ctggtgaaat
    19021 gggcattatg tggtcactgg ggacaatcgc cgcgtatttc tgaactcgca gaacaaaata
    19081 aaattattgc ttataactat ccacaaggtg tacttacaca aaccttacgc gccgccgcag
    19141 cccaccagcc tggtattatt agtgatattg gcatcgggac atttgtcgat ccacgccagc
    19201 aaggcggcaa actgaatgaa gtcacgaaag aagacctgat taaactggtc gagtttgata
    19261 acaaagaata tctctattac aaagcgattg cgccagatat cgccttcatt cgcgctacca
    19321 cctgcgacag tgaaggctac gccacttttg aagatgaggt gatgtatctc gacgcattgg
    19381 ttattgccca ggcggtgcac aataacggcg gtattgtgat gatgcaggtg cagaaaatgg
    19441 ttaagaaagc cacgctgcat cctaaatctg tccgtattcc gggttatctg gtggatattg
    19501 tggtggtcga tccggatcaa agccaactgt atggcggtgc gccggttaac cgctttattt
    19561 ctggtgactt cacccttgat gacagcacca aacttagcct gcccctaaac caacgtaaat
    19621 tagttgcgag gcgtgcatta ttcgaaatgc gtaaaggcgc ggtggggaat gtcggcgtcg
    19681 gtattgctga cggtattggc ctggtcgccc gggaagaagg ttgtgctgat gactttattc
    19741 tgacggtaga aacaggtccg attggcggaa ttacttcaca ggggatcgcc tttggcgcga
    19801 acgtgaatac ccgtgccatt ctggatatga cgtcccagtt taatttttat cacggtggtg
    19861 gtctggatgt ttgttatttg agttttgctg aagtcgacca gcacggtaac gtcggcgtgc
    19921 ataaattcaa tggcaaaatc atgggtaccg gtggatttat tgatatcagt gccacctcga
    19981 agaaaattat tttctgcggc acgttaaccg cgggcagttt aaaaacagaa attgccgatg
    20041 gcaaattaaa tatcgtccag gaaggacggg tgaagaaatt tattcaggaa ctaccggaaa
    20101 ttacttttag cggaaaaatc gcgctcgaac gggaactgga tgttcgttac atcaccgaac
    20161 gtgctgtatt cacgctgaaa gaagacggcc tgcatttaat cgaaatcgcc cctggcgtcg
    20221 atttacaaaa agatattctc gacaaaatgg atttcacccc agtgatttcg ccagaactca
    20281 aactgatgga cgaaagatta tttatcgatg cggcgatggg ttttgtcctg cctgaagcgg
    20341 ctcattaaca ggagtatcac gatggatttt tctttaactg aagaacaaga actgctgctg
    20401 gccagtattc gcgaactgat tacgactaac tttgcggaag agtatttccg cacctgcgat
    20461 caaaacggga catatccgcg tgagtttatg cgggcgctgg cggataacgg catttctatg
    20521 cttggcgtgc cggaagagtt cggtggcatc cctgcggatt acgtcaccca gatgctggcg
    20581 ctgatggaag tgtcaaaatg cggtgctccg gcatttttga ttaccaacgg tcaatgtatt
    20641 cacagtatgc gccgtttcgg ttctgcagag cagctacgta aaacggcaga gagcaccctg
    20701 gaaacgggtg atcccgccta tgccctggcg ttgacggagc caggcgcagg ctcagacaac
    20761 aacagtgcca ctaccactta cacgcgtaaa aacggcaagg tttacatcaa cggacagaaa
    20821 acctttatta ccggtgcgaa agagtacccg tatatgctgg tgttggcgcg cgatccgcaa
    20881 ccgaaagatc ccaaaaaagc cttcaccctg tggtgggtcg actccagtaa gcccggcatt
    20941 aagattaacc cgctgcataa aatcggctgg catatgctca gcacctgcga agtctatctc
    21001 gacaacgtgg aggttgaaga gagcgacatg gtgggcgaag aaggaatggg tttcctcaat
    21061 gtgatgtaca actttgagat ggagcgcctg atcaacgccg cgcgcagcac cggctttgcc
    21121 gaatgcgcct ttgaagatgc cgcccgctat gccaaccaac gtatcgcttt tggtaagccc
    21181 attggtcata accagatgat ccaggaaaaa ctggcgctga tggcgattaa gatcgacaac
    21241 atgcgcaaca tggtgctgaa agtggcatgg caagccgatc agcatcagtc actgcgcacc
    21301 agcgcggcgc tggcaaaact gtattgcgca cgtaccgcaa tggaagtcat tgatgatgcg
    21361 attcaaatca tgggcggtct gggctatacc gatgaagcgc gcgtctcccg cttctggcgt
    21421 gatgtccgtt gtgaacgtat cggcggcggt acagacgaaa ttatgattta cgtagcaggt
    21481 cggcagatcc tgaaagacta tcagaacaaa taatctgcag gcggcgcagc ttcttaacaa
    21541 actgcgccgc cagatttatc caacaagact taccggttga ggaaattcag ataggatttc
    21601 ttgttccgca aaacagtttt taaattgtct acggtaatcg ctgggcgaac aaccgacatg
    21661 gcgcaaaaac agtttggcaa agtgatcgac attttcataa cccacacgcc aggagatctc
    21721 tgcctgtgat aattcagtat tagtgagtga ccattttgct tccgtcatac gccgttgaat
    21781 aacatagtta ataggggaaa tacgatactc tttggtaaat tcatggcaaa tataactgac
    21841 gctggcacga aattttttcg ataactgttc taaagtgatt ttttcgcgat aattattact
    21901 cagatagaaa agaatatctt ttatcagaac atctttttta atatacccct gctccgaacg
    21961 ataagcattt ttaaaatttt cgtagtaaag aatggctaac gtataggcaa atgcatccca
    22021 taacgaagat gtttggctat ttttactttg cggcaaaatc acacttagct cattaaataa
    22081 ggtttttatg acttcttttc cctgccccgc ggcaattacc ggacaagaat gcggttgcag
    22141 tagctgattt tcctcagccc cctgaaactg aaagccgtac agcgcacagg tacacgtcgt
    22201 tgccggatcg ttaacgtcag aggccaccgc atgcagcctg ccgcgttcta tcaccacaat
    22261 gtcatccgca tgtgcgacat ataacgaaga atcgatggta aaccttgcaa cccctttctt
    22321 gacgtaaatc aactcggttt cattgtcatg aacgtgatgt ccggactccc attttggatc
    22381 atcgctaaat gcaaaacgtg aaagccgtgg cgttttaccg gcgacaaaca gcgtttcact
    22441 ggcgttatca aaacagcgtt gatacatgac aacctcccta ttccatgagc aagcaaaaac
    22501 aatatatgcc ggataaaata cggcgtgtat tgcggttata caaccgcgtt tagcgagagc
    22561 taaacaagat tatttacact gaatgcaaga ttgtacggtc atggaataac tatattgccg
    22621 caaattgcac ttttgtttta gcgatcgcat ttttttgcaa gattgttggc aaggaaaaca
    22681 gcttgctccg tcgaaaaccc ccaccgctat cgcacactat tttcaggcca tttttacctt
    22741 ccatcggaga tggttccgta tgcgactcac aggagaaatc atgaaaataa taacctgctt
    22801 taagctggtg cctgaagaac aggacattgt tgtcactcca gaatacaccc tgaatttcga
    22861 caatgccgac gccaaaatca gccagttcga tctcaatgcc attgaagctg caagccagct
    22921 cgcaacagat gacgatgaga tagccgcgct gaccgttggc ggctcattgt tgcagaactc
    22981 gaaagtgcgc agtgcgcaaa gatgtgctat cccgcgggcc gcacagcctg tatttggtgc
    23041 aggatgcgca acttgaacat gcactgcctc tcgataccgc aaaagcgctg gcggcagcaa
    23101 ttgaaaagat cggcttcgat ttactgatct ttggtgaagg ttccggcgac ctttatgccc
    23161 agcaggttgg cttgctggtc ggagaaattc tgcaacttcc ggtgattaat gcagtgagtg
    23221 ctattcagcg tcagggtaat acactggtga ttgaacgcac gcttgaagat gatgttgaag
    23281 ttattgaact ctctgttcca gccgtgctct gcgtcacctc cgatattaac gtgccacgca
    23341 ttccttcgat gaaagccatt ctcggcgcgg gtaaaaaacc ggtaaatcag tggcaggcaa
    23401 gtgatattga ctggagccag agcgcgccac ttgctgaact ggtcggcatt cgcgtaccgc
    23461 cgcaaacaga acgtaagcac atcattatcg ataacgattc gccggaggcc attgccgagc
    23521 tggcggaaca tctgaagaaa gccctgaact gaagcctacg gagaagaaac gatgagtcaa
    23581 ttaaacagcg tctgggtctt tagcgataat cctgaacgtt atgctgaact gtttggcggc
    23641 gctcagcaat ggggccaaca ggtgtatgcc attgtacaaa ataccgacca ggcgcaggca
    23701 gttatgcctt atggtccaaa atgtatttat gttcttgagc aaaacgacgc gctgcaacgc
    23761 actgaaaatt acgccgaaag cattgctgcc ccgctgaaag ataaacaccc cgctatgttg
    23821 ctgttggtcg cgacgaaacg tggtaaagct ctggcagcac ggttaagtgt gcaactgaat
    23881 gcggcgctgg tgaacgatgc cacggcggtg gatattgtcg atggtcacat ttgcgccgaa
    23941 caccggatgt atggcgggtt agcgttcgct caggaaaaga tcaacagccc gctggcgatc
    24001 attacccttg cacccggtgt tcaggaaccg tgcaccagtg atacctctca tcagtgcccg
    24061 acagaaacgg taccttatgt tgctccgcgt catgaaattc tctgtcgcga acgccgtgcg
    24121 aaagccgcaa gcagcgtgga cctgagcaaa gcaaaacgtg tggttggcgt cggtcgtggt
    24181 ctggcggcgc aggatgacct gaaaatggtc cacgaactgg cggcggtgct gaatgctgaa
    24241 gtcggctgtt cacgtccaat tgccgaaggc gagaactgga tggagcgtga acgttatatc
    24301 ggtgtctccg gcgtgttgct gaaatccgat ctctacctga cgctggggat ctccgggcag
    24361 atccagcata tggttggcgg caacggcgca aaagtgattg tcgccatcaa taaagataaa
    24421 aatgcgccaa tcttcaatta tgccgactac ggtctggtgg gcgatatcta caaagtcgtc
    24481 cctgccctga ttagccagtt gagccgctaa ttcccctttc aaccacatct cccgctgtaa
    24541 acgcagcggg aagggagcat aacgcatgtc ggatgacaaa tttgatgcca ttgtggtcgg
    24601 tgcggacgtt gctagtagcg ttgccgcact ggtcatggcg cgagccggac tggatgtcct
    24661 ggtgatagaa cgcggcgaca gtgccggatg taaaaacatg accggcgggc gtctttatgc
    24721 ccacacactt gaagcaatca ttccaggctt tgcagtatca gcgccggtag aacgcaaggt
    24781 cacacgcgag aaaatctcct tcttaaccga agaaagcgcc gttaccctcg attttcaccg
    24841 cgagcaacca gatgttccgc aacacgcatc ttataccgta ttgcgtaatc gtctggaccc
    24901 gtggttgatg gaacaagccg agcaggcggg cgcacagttt atcccgggcg ttcgcgtcga
    24961 tgcgttggtt cgtgaaggaa acaaggtcac tggcgtgcag gccggggatg atattctcca
    25021 agcgaatgtg gtggttctag ctgatggcgt aaactcgatg cttggccgct cgctgggaat
    25081 ggttcccgct tccgatccgc atcattacgc tgttggtgtt aaagaggtta ttggcctcac
    25141 accagaacag atcaacgatc gctttaatat tacgggcgag gaaggtgccg cctggctgtt
    25201 tgccggctcc ccttctgacg gcctgatggg cgggggattt ctctatacca acaaggattc
    25261 catatccttg gggctggttt gtggattggg tgatatcgcc catgcgcaaa aaagcgtgcc
    25321 gcaaatgctg gaagatttta aacaacaccc cgccattcgc ccactaatta gcggcggcaa
    25381 actgcttgaa tattccgcgc atatggtgcc agaaggcggt ctggcaatgg tgccgcagat
    25441 ggttaacgat ggcgtgatga tcgttggtga cgccgcaggc ttctgcctga atttgggttt
    25501 tacagttcgt ggcatggatt tagccattgc atcggctcag gctgccgcca caacggtgat
    25561 cgccgccaaa gaacgcgagg atttctccgc cagcagtctg gcgcaataca aacgtgagct
    25621 ggaacaaagc tgcgtcatgc gcgatatgca gcatttccgc aagatcccgg cgctgatgga
    25681 aaacccgcgc ctgtttagcc aatactcacg aatggtcgcc gacatcatga acgagatgtt
    25741 caccattgac ggcaaaccaa accagccggt acgcaaaatg atcatgggac acgcgaagaa
    25801 aattgggctg atcaacttgc tgaaagatgg cattaaggga gcaaccgcgc tatgagccag
    25861 aacgctacgg ttaacgtcga catcaaatta ggcgtcaata aattccatgt tgatgagggc
    25921 cacccgcata tcattctggc ggcaaatccc gatatcaatg aattccgtaa attaatgaaa
    25981 gcttgccctg ccggacttta taagcaggat gacgccggca acattcattt cgattccgcc
    26041 ggttgtctgg agtgcggcac ctgtcgggtg ctgtgcggca acactattct cgaacagtgg
    26101 caatatcccg caggcacctt cggtattgat tttcgctacg gctaatcatg catcccacag
    26161 gcccgcatct cgggcctgat gttctgtttc gagagtccaa aatgaaagtg acattaacgt
    26221 tcaacgaaca acgtcgtgcg gcgtatcgtc agcaagggtt atggggcgat gcttcactgg
    26281 ccgattactg gcagcagacc gctcgtgcga tgccagacaa aattgccgtg gtcgacaatc
    26341 atggtgcaac gtacacctat agcgcgctcg atcacgccgc gagctgtctg gcaaactgga
    26401 tgttagcgaa gggtattgaa tcaggcgatc gcatcgcatt tcaactgcct ggctggtgtg
    26461 aatttaccgt tatctatctt gcctgcctga aaatcggtgc ggtttccgtg ccgctgttgc
    26521 cttcctggcg ggaagcagaa ctggtgtcgg tgctcaataa gtgtcaggca aaaatgttct
    26581 ttgcaccgac gttgtttaaa caaacacgtc cggtagattt aatcctgccg ctgcaaaatc
    26641 agcttccaca actacaacaa attgtcggcg tggacaaact ggctcccgcc acctcttccc
    26701 tctcattaag tccgattatc gccgacaata cctcactgac cacggcgata acgacccacg
    26761 gcgatgaatt agctgcggtg ctgtttacct ccggaaccga gggtctgcca aagggcgtga
    26821 tgctaacgca taacaatatt ctcgccagtg agcgggctta ttgcgcgcga ctgaatctga
    26881 cctggcagga tgtctttatg atgcctgcgc cacttggtca cgcaacgggc tttctgcatg
    26941 gcgtaacggc accattctta attggcgctc gcagcgtgtt gttagatatt ttcactcctg
    27001 atgcgtgtct cgcgctgctt gagcagcagc gttgcacctg tatgctaggc gcaacgccgt
    27061 ttgtctatga tcttttgaat gtactagaga aacaacccgc ggacctttca gcgctgcgtt
    27121 tctttctttg cggcggtacc acaatcccca aaaaagtggc acgtgaatgc cagcagcgcg
    27181 gcattaaatt attaagtgtt tatggttcca cagaaagttc gccgcatgcg gtggtgaatc
    27241 tcgatgatcc tttgtcgcgc tttatgcaca ccgatggtta cgctgccgca ggtgtagaga
    27301 ttaaagtggt cgatgacgca cgcaagacct taccgccagg ttgcgaaggt gaagaagcct
    27361 cgcgtggccc caatgtgttt atggggtatt ttgatgaacc tgaattaacc gcccgtgcgc
    27421 tggacgaaga aggctggtat tgcagcggcg atctctgccg tatggatgag gctggctata
    27481 taaaaattac cggacgcaaa aaagatatta ttgtccgcgg cggcgaaaat attagcagcc
    27541 gtgaagtgga agatatttta ttgcagcatc ctaaaattca cgatgcctgt gtggttgcaa
    27601 tgcccgatga acgtttaggt gaacgatcat gcgcttatgt cgtgctgaaa gcgccgcatc
    27661 attcattatc gctgga
//
LOCUS       DAJTMC010000038        27527 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.038, whole genome shotgun sequence.
ACCESSION   DAJTMC010000038 DAJTMC010000000
VERSION     DAJTMC010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27527
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.038"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(174..704)
                     /gene="ppa"
                     /locus_tag="OO130_001451"
     CDS             complement(174..704)
                     /gene="ppa"
                     /locus_tag="OO130_001451"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HCR5364913.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            1014..1970
                     /gene="ytfQ"
                     /locus_tag="OO130_001452"
     CDS             1014..1970
                     /gene="ytfQ"
                     /locus_tag="OO130_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HCR5364914.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTVGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENRIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMIDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            2110..3612
                     /locus_tag="OO130_001453"
                     /pseudo
     CDS             2110..3612
                     /locus_tag="OO130_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026286.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
     gene            3626..4648
                     /locus_tag="OO130_001454"
     CDS             3626..4648
                     /locus_tag="OO130_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5364915.1"
                     /translation="MPQSLPDTTTPKRRFRWPTGMPQLVALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     EVSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITSGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSRDKT"
     gene            4635..5630
                     /gene="yjfF"
                     /locus_tag="OO130_001455"
     CDS             4635..5630
                     /gene="yjfF"
                     /locus_tag="OO130_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
                     /protein_id="HCR5364916.1"
                     /translation="MIKRNLPLMITIGVFVLGYFYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVVGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPIYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQR"
     gene            complement(5663..6661)
                     /gene="fbp"
                     /locus_tag="OO130_001456"
     CDS             complement(5663..6661)
                     /gene="fbp"
                     /locus_tag="OO130_001456"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HCR5364917.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            6837..8210
                     /gene="mpl"
                     /locus_tag="OO130_001457"
     CDS             6837..8210
                     /gene="mpl"
                     /locus_tag="OO130_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HCR5364918.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLDPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADTANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(8293..8844)
                     /locus_tag="OO130_001458"
     CDS             complement(8293..8844)
                     /locus_tag="OO130_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HCR5364919.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEE"
     gene            8938..10290
                     /gene="pmbA"
                     /locus_tag="OO130_001459"
     CDS             8938..10290
                     /gene="pmbA"
                     /locus_tag="OO130_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HCR5364920.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGVDCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            10474..10860
                     /gene="cybC"
                     /locus_tag="OO130_001460"
     CDS             10474..10860
                     /gene="cybC"
                     /locus_tag="OO130_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HCR5364921.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVVEKADN
                     AAQVKDALTKMRAAALDAQKATPPKLEGKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(10905..11369)
                     /gene="nrdG"
                     /locus_tag="OO130_001461"
     CDS             complement(10905..11369)
                     /gene="nrdG"
                     /locus_tag="OO130_001461"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HCR5364922.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDQIINDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVQRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(11468..13606)
                     /gene="nrdD"
                     /locus_tag="OO130_001462"
     CDS             complement(11468..13606)
                     /gene="nrdD"
                     /locus_tag="OO130_001462"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HCR5364923.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYSNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(14000..15655)
                     /gene="treC"
                     /locus_tag="OO130_001463"
     CDS             complement(14000..15655)
                     /gene="treC"
                     /locus_tag="OO130_001463"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HCR5364924.1"
                     /translation="MTNPPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAVLKKVCEFWADRGVDGLRLDVVNLISKDPRFPDDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            complement(15705..17126)
                     /gene="treB"
                     /locus_tag="OO130_001464"
     CDS             complement(15705..17126)
                     /gene="treB"
                     /locus_tag="OO130_001464"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HCR5364925.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNARQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAETFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPNYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(17227..18192)
                     /gene="treR"
                     /locus_tag="OO130_001465"
     CDS             complement(17227..18192)
                     /gene="treR"
                     /locus_tag="OO130_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HCR5364926.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPEFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLSRSFPER"
     gene            18377..18430
                     /gene="mgtL"
                     /locus_tag="OO130_001466"
     CDS             18377..18430
                     /gene="mgtL"
                     /locus_tag="OO130_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HCR5364927.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            18571..21267
                     /gene="mgtA"
                     /locus_tag="OO130_001467"
     CDS             18571..21267
                     /gene="mgtA"
                     /locus_tag="OO130_001467"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HCR5364928.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNTVVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQLSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(21473..21859)
                     /gene="ridA"
                     /locus_tag="OO130_001468"
     CDS             complement(21473..21859)
                     /gene="ridA"
                     /locus_tag="OO130_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HCR5364929.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYETFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAIRR"
     gene            complement(21932..22393)
                     /gene="pyrI"
                     /locus_tag="OO130_001469"
     CDS             complement(21932..22393)
                     /gene="pyrI"
                     /locus_tag="OO130_001469"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HCR5364930.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(22406..23341)
                     /gene="pyrB"
                     /locus_tag="OO130_001470"
     CDS             complement(22406..23341)
                     /gene="pyrB"
                     /locus_tag="OO130_001470"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HCR5364931.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(23345..23479)
                     /gene="pyrL"
                     /locus_tag="OO130_001471"
     CDS             complement(23345..23479)
                     /gene="pyrL"
                     /locus_tag="OO130_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HCR5364932.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            complement(23759..24154)
                     /locus_tag="OO130_001472"
     CDS             complement(23759..24154)
                     /locus_tag="OO130_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCR5364933.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQFEDIMTVKNEIFSAP
                     SYPTWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            complement(24285..24998)
                     /gene="bdcA"
                     /locus_tag="OO130_001473"
     CDS             complement(24285..24998)
                     /gene="bdcA"
                     /locus_tag="OO130_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR5364934.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADKDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMSHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            25069..25662
                     /locus_tag="OO130_001474"
     CDS             25069..25662
                     /locus_tag="OO130_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR5364935.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGNKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLAEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAVQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTILKE"
     gene            25807..26259
                     /gene="tabA"
                     /locus_tag="OO130_001475"
     CDS             25807..26259
                     /gene="tabA"
                     /locus_tag="OO130_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HCR5364936.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPHEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGTPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPARVRKAVVKMLMA"
     gene            26382..27527
                     /locus_tag="OO130_001476"
                     /pseudo
     CDS             26382..27527
                     /locus_tag="OO130_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234052.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
BASE COUNT         6604 a         6815 c         7277 g         6831 t
ORIGIN      
        1 tccagcaagt ggctatgaac agcaaaatgg tggtcgggtt tgcttgttct taccgttcaa
       61 gccttgcaac cgctgagaat aatgatggtg gaatagagag tttatttatc gcgggcataa
      121 aaaaaccctt actaaccgaa gcccggcgtt cagggttatt gcgctagaag aacttattta
      181 ttctttgcgc gctcgaagga agcaacgatt tcagctttag cggcttctgc gttttcccaa
      241 ccttcaactt tcacccactt gcctttttcg aggtctttgt agtgctcgaa gaagtgagcg
      301 atttgcgctt tcagcagttc aggcagatcg tttacgtctt taatgtgatc gtattctttg
      361 ctcagcttgc tgtgcggaac cgcaaccagt ttcgcatctt caccggcttc gtcggtcatt
      421 ttcagaacgc caaccggacg gcaacggatc acagaaccag gctgcagcgg gtacggagtc
      481 gggaccagta cgtcaaccgg gtcaccgtcc agagacaggg tgtggttgat gtaaccgtag
      541 ttgcacggat agaacatcgc ggtggacatg aagcggtcaa cgaacagtgc gccgctctct
      601 ttgtcgattt cgtatttgat cggatctgcg ttagccggga tctcaataac aacgtagatg
      661 tcttccggca gatctttacc cgcagggacg ttgagtaagc tcatgtctgt ttcctttaaa
      721 aatatgtggc aaacaagtgc cgagtattat agccaactcg cgccgaatgt ctttgcttgt
      781 tttcgtcttc attttccttt ttcgccagtt ttaagacggt atacagagca ggaaaatcca
      841 taaccgtagc cgcatttttc atagtgagat gaaagcgatt acaaacttgt gattaacgtt
      901 ttatttactt ttttgaagtg tgatgtaacg caatccgtta cataacgaat tgtctatagt
      961 tttttcgcga acatcttttc accaataata attaccccga ctaggacaac cctatgtgga
     1021 aacgcttact tgtagtctct gcagtctcgg cagccatgtc gtctatggcg ttggccgctc
     1081 cattaaccgt aggattttcg caggtcggat cggaatccgg ctggcgcgcc gcagaaacca
     1141 atgtggcgaa aagtgaggcc gaaaaacgcg gaattacgtt gaaaattgcc gatggtcagc
     1201 aaaagcagga aaaccggatt aaagcggtac gttccttcgt tgcacaaggg gtggatgcga
     1261 tctttatcgc tccagtggtt gcgacgggtt gggaaccggt attaaaagag gcgaaagatg
     1321 ccgaaatccc ggtcttcttg ctcgaccgtt ccatcgatgt gaaagacaaa tctctctata
     1381 tgaccaccgt tactgccgac aacatcctcg aaggtaagtt gatcggtgac tggctggtaa
     1441 aagaagtgaa tggcaaacca tgcaacgtgg tggagctgca gggcaccgtt ggggccagcg
     1501 tcgccattga ccgtaagaaa ggctttgccg aagccattaa gaatgcgcca aatatcaaaa
     1561 ttatccgctc gcagtcaggt gacttcaccc gcagtaaagg caaagaagtc atggagagct
     1621 ttatcaaagc ggaaaacaac ggcaaaaaca tctgcatggt ttacgcccat aacgacgaca
     1681 tggtgattgg tgcaattcag gcaattaaag aagcgggcct gaaaccgggc aaagatattc
     1741 tgacaggttc tatcgatggc gtaccggata tctacaaagc gatgattgat ggcgaagcga
     1801 acgccagtgt tgaactgacg ccgaatatgg cgggacccgc ttttgacgcg ctggagaaat
     1861 acaaaaaaga cggcaccatg cctgaaaagc tgacgttaac caaatccacc ctttacctgc
     1921 ctgataccgc aaaagaagag ttagagaaga agaaaaatat ggggtattga gggttgctat
     1981 gcctgatgcc gattcgtagg ccggataagg cgctcgcgcc gcatccggcg atggtgcact
     2041 gaagcctgat gcgacgctta ccgcgtctta tcatgcctac tgggagcacg ctttacaccg
     2101 ggggaaacca tgacgaccga ccaacaccag gaaatcctcc gcaccgaagg attaagtaaa
     2161 tttttccccg gcgtcaaagc gttagacaac gttgatttca gcctgcgccg tggcgaaatc
     2221 atggcgctgc tcggtgaaaa cggggcggga aaatcaacgc taatcaaagc attaactggt
     2281 gtataccacg ccgatcgcgg caccatctgg ctggaaggcc aggttatctc accgaaaaat
     2341 accgcccacg cgcaacaact cggcattggc accgtctatc aggaagtcaa cctgctaccc
     2401 aatatgtcgg tcgctgataa tctatttata ggccgcgaac ccaaacgctt cggccttcta
     2461 cgccgcaaag agatggaaaa gcgcgccacc gaactgatgg catcttacgg tttctccctc
     2521 gacgtgcgcg aaccgctcaa ccgcttttca gtcgcgatgc agcaaatcgt cgccatttgc
     2581 cgggctatcg atctctccgc caaagtgctg atcctcgatg aacccaccgc cagcctcgac
     2641 acctaggaag tggagctatt atttggcctg atgcgtcagt tgcgcgatcg cggcgtcagc
     2701 ctgatctttg tcactcactt tctcgatcag agctatcagg tcagcgatcg gatcaccgtt
     2761 ttacgcaacg gcagtttcgt aggctgtcgg gaaacgcgcg agctaccgca gatcgaactg
     2821 gtaaaaatga tgctggggcg cgagctggat acccacgcgc tacagcgtgc cgggcgaaca
     2881 ttgttgagcg acaaacccgt tgccgcgttc aaaaattacg gcaaaaaagg aacgatagca
     2941 ccgtttgatc tcgaagtgcg ccccggcgag atcgtcggcc tggcaggctt gctgggatcc
     3001 ggacgtaccg aaaccgccga agtgatcttc ggtatcaaac ctgctgacag cggcacggcg
     3061 ttgatcaaag gcaaaccgca aaacctgcga tcgccacatc aggcttcggt actgggcatc
     3121 ggcttctgcc cggaagacag gaaaaccgat ggcatcatcg ctgccgcctc ggtgcgggaa
     3181 aatatcatcc tcgctctcca ggcccagcgc ggctggctac gacccatttc ccgcaaagag
     3241 cagcaagaga ttgccgaacg ctttatccgc cagcttggca ttcgcacacc ttcaactgaa
     3301 caaccgattg aatttctctc cggcggcaat cagcaaaaag tgttgctttc acgttggcta
     3361 ctgacccgac cgcaatttct gatcctcgat gagccaaccc gtggcattga tgttggtgcc
     3421 cacgccgaga tcatccgcct gattgaaacg ctatgcgccg atggtctggc gctgctggtg
     3481 atctcctccg aactggaaga actggtgggc tatgccgacc gggtgatcat catgcgcgat
     3541 cgcaaacagg tggcggagat ccggctggca gagctttccg ttccggctat catgaacgcc
     3601 attgcggcgt aaggagaaca gtgtgatgcc tcaatctctt ccggacacca ctacgccaaa
     3661 aaggcgcttt cgctggccga cggggatgcc gcagctggta gcactattgc tggtgctgct
     3721 ggtcgacagc ctggtagccc cgcatttctg gcaggtagtg cttcaggatg ggcgtttgtt
     3781 cggtagcccc atagacattc ttaaccgtgc agcccccgtt gcgttactgg cgattggtat
     3841 gacgctggtg atcgccaccg gtgggattga tctctccgta ggggcggtga tggctatcgc
     3901 cggagccaca acggctgcga tgacggtcgc ggaagtcagc ctgccgattg ttttgttaag
     3961 cgccctaggc accggcatcc tggcgggatt gtggaacggc atactggtag cgatcctcaa
     4021 aattcagcca tttgtcgcca ccctgatcct gatggtcgcc gggcgcggcg tggcgcaact
     4081 gatcacctcc ggacagatcg tcacgtttaa ctcgccggat ctctcatggt ttggcagtgg
     4141 atcgctgttg ttcctgccaa cgccagtcat tattgcggta ctgacgcttc tcctgttctg
     4201 gctgttgacc cgcaaaacgg cactggggat gtttatcgaa gccgttggta tcaacattcg
     4261 ggcggcaaaa aatgccgggg taaacacgcg gatcatcgtc atgctcactt atgtgttgag
     4321 cgggctgtgt gcggcgattg cgggcattat cgtggcggcg gatattcgcg gtgccgatgc
     4381 caacaacgcc ggattatggc tggagctgga cgccattctc gcggtggtga ttggcggcgg
     4441 atcgctgatg ggcgggcgct ttaacctgct gctctcggta gtgggggcgc tgattattca
     4501 ggggatgaac accggaattt tgctttcggg ctttccaccg gagatgaacc aggtggtgaa
     4561 agcggtggtg gtgctttgcg tgctgattgt ccagtcgcaa cgctttatca gtctgattaa
     4621 aggagtacgt agccgtgata aaacgtaatt tgccactgat gatcaccatc ggcgtctttg
     4681 tgctgggcta tttctactgc ctgacgcagt ttcccggttt tgcttccaca agagtgatct
     4741 gcaatatcct gaccgataac gcttttctgg ggattattgc cgttggtatg acctttgtga
     4801 tcctctctgg cggtatcgat ctctccgtcg gttcggtgat cgcctttact ggcgtgtttc
     4861 tggcaaaagt ggttggcgat ttcggcctct cgccgctgct ggcgtttccg ctagtgctgg
     4921 tgatgggctg tgccttcggc gcatttatgg ggcttctgat cgacgccctg aagatcccgg
     4981 catttatcat tacgcttgcg gggatgttct ttttgcgcgg cgtcagctat ctcgtttcgg
     5041 aagagtcgat tccgataaac catcccattt atgacacgct ctcaagcctt gcgtggaaaa
     5101 tccccggcgg cggtcgcctt agtgcgatgg ggctgttgat gctggcggtg gtggttatcg
     5161 gtattttcct tgcgcatcgt acccgttttg gcaatcaggt gtacgctatt ggcggcaacg
     5221 cgacgtcggc gaacctgatg gggatttcta ctcgcagcac tactattcgc atttatatgc
     5281 tctccaccgg actggcaaca ctggcgggga ttgtcttctc gatttatacc caggccggat
     5341 atgcactggc gggcgtaggt gtggaactgg acgccatcgc gtcggtggtg attggcggta
     5401 cgttgttaag tggtggcgtc gggacggtgc ttgggacgct ttttggcgtg gcaattcagg
     5461 gactgatcca gacttacatc aactttgacg gcacgctgag ttcctggtgg acgaaaatcg
     5521 ccatcggtat tttgttgttt atttttatag cattacagcg tgggttaacg gtgctgtggg
     5581 agaatcgtca gagttcgcca gtgacaagag tcaacattgc gcagcgataa aaatgcctct
     5641 ccgtgtggag aggcgcagga gattacgcgt ccgggaactc acggataaag cgttcgacat
     5701 cttcaaccat atggtcgttg ccgacaaaga acgaacggcg ctggtgcaga gtttccggga
     5761 tgatatccag aatacgctct ttgccatcgc tcgctttacc gcccgcttgt tccgccagga
     5821 atgccatcgg gttgcactca taaagcaaac gcagtttgcc gtccgggtgg ctggcggtgc
     5881 ttgggtagag ataaataccg cctttcagca ggttacggtg gaaatccgca accagtgaac
     5941 cgatataacg tgaggtatac gggcggttgg tggatttatc ttcttcctgg cagaatttaa
     6001 tgtacttctt caccccgttc ggaaacttaa tgtagtttcc ttcgttgatg gagtaggttt
     6061 tgcctttctc cgggaagcgc atccgttcct ggcacaggca gaaaacgccc agcgaaggat
     6121 cgtaagtaaa ggcgtgaaca ccgcatccgg tggtgtaaac cagcatggta gaggagccgt
     6181 ataccacgta acctgccgca acctgtttgt taccaggctg gaggaaatct tcttccgtta
     6241 ccggcgtgcc gacgggcgta actcggcggt agatggagaa aatggtaccg acagagacgt
     6301 taacatcgat gttggacgag ccatccagtg ggtccatcag caccacgtat tttgcatgtt
     6361 cacagccttc aaagacaaca atctcatctt cttcttcaga ggcaatgcct gcaacgatat
     6421 cgcgtgcttt cagtgcggct ttcagttttt cattagcgaa caagtcgagt ttttgctgaa
     6481 cctcgccctg cacgttctca gcaccgctgg cacccaggat atcaaccagt cctgctttgt
     6541 tgatatcgcg atggataatc ttggcgccca gttttattgc cgacagcaaa gcagtgagct
     6601 caccggtagc atgagaaaac tcgtgctgct tttcgacaat aaattcacct aacgttttca
     6661 taaaactttc cctgcaatgt ttatggagta aagcgaccgc aacaatctta acaaataatc
     6721 tcaatgttgc gctcaggtga atcgcgccag caaattacgg attatcctga aatgcgtttc
     6781 tcacttgccc gacatatgcg taaaatgagc ggcagattaa aaaaggatag tgacgtatgc
     6841 gcattcatat tttaggaatt tgtggcacgt ttatgggcgg tctggcgatg ctggcgcgcc
     6901 agttaggcca tgaagtaacg ggttcggacg ccaatgtgta tccgccgatg agcaccttac
     6961 ttgagaagca aggcattgaa ctgattcagg gttacgatgc cagccagctc gatccgcagc
     7021 cggatctggt gattattggc aacgccatga cccgtggaaa tccgtgtgtg gaagcggtac
     7081 tggaaaaaaa catcccttat atgtcaggtc cacagtggct gcacgatttt gtgctgcgcg
     7141 accgctgggt gctggccgtt gccggtacac acggcaaaac caccaccgcg ggaatggcga
     7201 cctggattct ggaacagtgt ggttacaaac cgggatttgt gatcggcggt gtgccgggga
     7261 actttgaggt ttcggcgcgt ctgggcgaaa gcgacttctt tgttatcgaa gcggatgagt
     7321 acgactgcgc cttcttcgac aaacgctcta aatttgtcca ttactgcccg cgtacgctga
     7381 tcctcaacaa ccttgagttc gatcacgccg atatctttga cgacctgaaa gcgatccaga
     7441 aacagttcca ccatctggtg cgtatcgttc cggggcaggg ccgtattatc tggccggaaa
     7501 atgacatcaa cctgaaacag accatggcga tgggctgctg gagcgagcag gagctggtgg
     7561 gtgagcaggg gcactggcag gcgaaaaagc tgaccactga tgcttccgaa tgggaagtct
     7621 tgctggatgg cgaaaaagtg ggcgaagtga aatggtcgct ggtaggcgaa cataatatgc
     7681 ataatggcct gatggcgatt gcggcggccc gccatgttgg cgtagcgccg gcagataccg
     7741 ctaacgcgct gggttcgttt attaatgctc gtcgccgtct ggagttgcgt ggtgaagcga
     7801 atggcgtcac ggtatatgac gattttgccc atcacccgac ggcgattctg gcaacgctgg
     7861 cggcgctgcg tggcaaagtt ggtggtacag cgcgcattat tgctgtgctg gaaccgcgct
     7921 cgaataccat gaaaatgggg atctgcaaag acgatttggc accttcatta ggtcgtgccg
     7981 atgaagtctt cctgctgcaa ccggcgcata ttccgtggca ggtggcagaa gtggcagaag
     8041 cctgcgttca gcctgcacac tggagtggcg atgtggatac gctggcagat atggtggtga
     8101 aaaccgctca gcctggcgac catattctgg tgatgagcaa cggcggtttt ggtgggatcc
     8161 atcagaaact gctggatggt ctggcgaaga aggcggaagc tgcgcagtaa ttcggcctca
     8221 gcctgagata gcattgccgg ataaggcgtt tacgccgcat ccggcaaacc atactcacat
     8281 caacaacgaa aattactctt cattctccgc cagttcgcgc agatactgga aaatctggcg
     8341 tgcggatttc ggcggcttat tcccttcttt ctctttcttc gcgttgcgga tcagagtacg
     8401 cagttgctga cgatcggcat cgggccacag atttaatacc tcagcgatgg cgtcatcacc
     8461 ttgatcgatc agacgatcgc gcaggttttc cagtttatgg aacagcacca cctgctggtt
     8521 gtgacggttt ttcagcttat ccagcgcctg acggatcggt tctacatcgc gctggcgcag
     8581 catcttaccg atgagctgca actggcggcg gcgaccttcc atcttaatac gctgggcgag
     8641 ctcaatagcc gcgcgcagat ccgcatctaa cgggatctta tccagcgcgt ttttccccag
     8701 atcaacaatt tccgcgccaa ggcgttttag ctcctcggca tcacgtttaa tttcactttt
     8761 actgacccag ataatttcat cgtcttcgtc ttcgatgtca tcaccgggaa cgtcgtcgag
     8821 ccagtcttcg ggctgcttag tcatctcagg ctccttaaaa aaagaggcta atgttaccag
     8881 ttaagatgcg cactgaaaaa cggttctctg ttagacttca gagaaactct ctacattatg
     8941 gcacttgcaa tgaaagtaat ctctcaagtt gaagcgcagc gcaagattct ggaagaagca
     9001 gtttccactg cgctggagtt ggcctcaggc aaatcggacg gtgcggaagt tgccgtcagc
     9061 aagaccaccg gcattagcgt aagcacgcgt tatggtgaag tggagaatgt tgaattcaat
     9121 agcgatggcg cgctggggat cactgtttat caccagaacc gcaaaggtag cgcatcatcc
     9181 accgatttaa gcccgcaggc cattgcccgt actgtacagg cggcgctgga tattgcccgt
     9241 tatacctcgc cagatccctg tgccggcgtg gcagacaaag agctgctggc ctttgacgca
     9301 ccagatctcg acttgttcca ccctgcggaa gtttccccgg atgaagccat tgaactggcg
     9361 gcccgcgcag aacaggcggc attgcaggcg gacaaacgca tcaccaatac cgaaggtggc
     9421 agctttaaca gccactacgg tgtcaaagtt tttggcaaca gccacggcat gttgcagggt
     9481 tactgctcaa cgcgtcattc gctctccagc tgtgtaattg ccgaagaaaa tggcgatatg
     9541 gagcgtgatt acgcctacac cattggtcgt gcgatgagcg atctgcaaac gccagagtgg
     9601 gttggggtcg actgtgctcg ccgtacttta tcgcgcctgt caccgcgtaa actctccacc
     9661 atgaaagcgc cggtcatttt tgccaatgaa gtggcaaccg ggctttttgg ccatctggtg
     9721 ggggcgatag cgggtggatc ggtttaccgt aaatctacct tcctgctgga ttcgctgggt
     9781 aaacaaattc tgccggactg gctgaccatt gaagagcatc cgcatctgct gaaagggctg
     9841 gcgtcgacgc cattcgacag cgaaggtgtg cgcaccgagc gtcgcgatat tattaaagat
     9901 ggcatcctga ctcagtggct gctgaccagc tattcggcgc gaaaactggg gctgaaaagc
     9961 accggacatg cgggcggtat tcacaactgg cggattgccg gacaaggtct aagcttcgag
    10021 cagatgctca aagagatggg caccgggctg gtggtgacgg aattgatggg ccagggcgtg
    10081 agtgcaatta ccggtgatta ttcccgtggt gcggcgggct tctgggtaga aaacggcgaa
    10141 attcagtatc cggttagcga aatcaccatc gcaggtaatt taaaagatat gtggcgcaat
    10201 attgtcaccg tcggtaacga tattgaaaca cgcagtaata tacagtgtgg ttctgtgctg
    10261 ttgccggaga tgaaaatcgc cggacagtaa aaaaactggc gcgacctgtc gcgctataaa
    10321 ctaaaaaatt acacaaaatc attcgcactg catcgagacg gaaagtgaat gagggccggg
    10381 gagcgtactc gcagtacgtg acccggtcga atgagcgtag tcgtcgatga ggcagtgtga
    10441 atgatgaagt gtaaataaaa aaggaagtga gcaatgcgaa aaagcctgtt agctattctt
    10501 gcagtctcct cgttggtatt cagttcggcg tcgtttgccg ctgatcttga agacaatatg
    10561 gaaaccctca acgacaattt aaaagtggtc gaaaaagccg ataacgcggc gcaagtcaaa
    10621 gacgcgttaa cgaagatgcg cgccgcagcg ctggatgcgc aaaaagcaac gccgccgaag
    10681 ctcgaaggca aatcaccgga cagcccggag atgaaagact tccgtcacgg tttcgatatt
    10741 ctggtcggtc agattgacga cgcgctgaag ctggcaaatg aaggtaaagt aaaagaagcg
    10801 caggctgccg cagagcaact gaaaacgacc cgcaacgcct atcaccagaa gtatcgttaa
    10861 ttcctcattt ccctgttgcc tgcactcagg taacagggaa cttatcatcg caaatgatgc
    10921 accacctgat tgctgctgcc gcgccagatc agggaagggt cttttaaatc ctgcacaaat
    10981 ttgccgtcga ccagcacgtt aatcaaatca acaacctgca tttgcgctgc gttgagttcg
    11041 tcgagtttat agcctgtcca cacccagatg tctttacccg gacactcggc gcggatgcgt
    11101 tgtaccagtt tcagaatatc cggcacgttt tgcggatgca gcggatcgcc gccggagagg
    11161 gaaataccct ggcgtttgat acgagtgtca ttcagatcgt taatgatctg gtcttccatt
    11221 gctttagtaa atggctgacc ggaatttacc cgccatgtgc ttttgttata gcaaccgggg
    11281 cattcatgaa cacacccgga gacaaacaga gtgcaacgag tgccggggcc gttgacgatg
    11341 tcgacaggat agtactgatg ataattcatt atttcgcctg gcttatcagt ccgcgtgtag
    11401 actgcatccg gtaatcaatg cctgatgcga cgctttcaca tcacatccgg catttgctta
    11461 gtgcagatca acctatctgc ccatttccca aatgtttaac gcggcgctta acttcttcct
    11521 gcttgccggc gttaaacgga cgtgcatccg ggctacctaa atatccgcac acgcggcgag
    11581 ttaccgacac acgggaggcg tcatggttac cacatttcgg gcaggtgaag cctttgctgg
    11641 tgcactcgaa ctcaccggta aagccacact cgtagcactc atcaatcggt gtattggtgc
    11701 cgtaatacgg tacatgctga tagctgtaat cccagacatc ttccagcgcc ttcaggttgt
    11761 gctgaatgtt tgaatactcg ccgtagcaaa tgaaaccacc gttcgccagc ggcgggtaag
    11821 gcgcttcaaa gtcgatcttg tcgtacgggt tcaccttctt ctccacatcg aggtggaaac
    11881 tgttggtgta gtaaccttta tcggtcacgc ccggcaccac gccaaactca gcagtatcga
    11941 gacggcagaa gcggtcgcac aggttttcac tcggcgtgct gtagagactg aaaccataac
    12001 ccgtttcttc cttccactga tccactgcct gacgcagacg ttcaacaatg gcgatacctt
    12061 tcgcgcgaag ctgctcgttg tcgtaaacat gctcgccgcc gaacagcgcg ttaatggttt
    12121 cgtggatgcc gatgtaaccc agcgaaatag acgcacgacc gtttttgaag atttcagaaa
    12181 catcatcgtc agcattgaga cgcacgccac aagcaccttc catatagagg atcggggcca
    12241 cgcgcgcttt cacgccttcg agacgagcga tacgggtcat cagcgcctta cgtgccagca
    12301 ccagacgttc atccagcagc ttccagaagg tggcttcatc gccttttgct tccagagcaa
    12361 tacgcggcag gttcaggctg atcacgccga ggttgttacg accatcgtgg atctgctcgc
    12421 cgttttcatt ttcccacacg ccgaggaagc tgcggcagcc catcggagtt ttaaacgaac
    12481 cggtgacttt cactacctga tcgtagttca ggatatccgg atacatgcgc ttgcttgcgc
    12541 actccagcgc cagctgtttg atgtcgtagt tcggatcgcc ttttttatgg ttcaggccat
    12601 cgcgaatcgc aaacaccagt ttcgggaaca ccgcagtttt acggttttta ccgaggcctg
    12661 cgatacggtt acgcaggata gattcctgaa tcaggcgcga ctcccagctg gtgcccagac
    12721 caaaaccaaa ggttacaaac ggcgtctgac cgttggcggt gtgcagggtg tttacttcgt
    12781 actccagcga ctggaaggca tcgtagcact ctttaatggt acgagagtta gcgtagcctt
    12841 cggcgtccgg gatgttccac tcttctgcgg tcttgcgatg tttgttgtag ctggcggtga
    12901 caaacggtgc cagcacttca tcgatacggt taatggtggt gccgccataa atatggctgg
    12961 cgacctgagc aataatctgc gcagttacag cggttgccgt agagatcgac ttcggcggtt
    13021 caatctcggc gttccccatc ttaaagccct gggtcagcat gcctttcagg tcgatcaaca
    13081 tgcagttgaa catcgggaag aacggcgagt aatcgagatc gtgatagtga atatcgccac
    13141 gctcatgtgc ctgcaccacg tcacgcggca gcaggtgctg acgtgcatag tgtttagcca
    13201 cgatcccggc cagcaggtcg cgctgggttg gaatcacctt gctgtctttg ttggcgtttt
    13261 cgttgagtaa cgaggcgttg gtctgctcga ccagaccacg gatctcctga ttcaggcgac
    13321 cgcgtttttc acgttcaatg tcgcgatcgt gacggtactc gatgtaagca cgagccagtt
    13381 gtttgtatgg acctgacatc agctggtttt caacagcggt ctggatctca ttgatatcca
    13441 cctggttgcg gccctgcatc tgctcgctga caactgcagc aacagtggcg caataatcgg
    13501 catcatcgac ttccgctgct ttagctgcac gcagaatcgc ttctttgatg cgctctgatt
    13561 taaacggcac tttgcagccg tctcgtttca tcacatgcgg tgtcatgatc gctccatatt
    13621 tttaagaaca ggttatccac agaaattggg aaaggcgttt tccggttttt tcgtttcgtt
    13681 ttccggtgct ttccgcaatc gccatccgct tttccacaac gccgcacctt tttcgtgggg
    13741 agctgtagta gcaattatag tcgattaata caacatattg ggttgggacg cattttaaag
    13801 tctatatata gtgctttgca tcaaggatgt ttgaactttt tttgatgtag ctcaaagtaa
    13861 aaagcagagc gtacggatga cgggcgctac agcgttatgt aaatttttta atgaatttgc
    13921 tggttgaaaa ataaacaaaa acaacatgct gacagacaaa accccggaat gaccggcatt
    13981 accggggctt agggaagatt tacttctgta accaccagac agcctcaaaa ggccgtaaat
    14041 tcatggcaca gggttgtggt gaggcttctt cgtagttatg catcacaagc tgccagttgc
    14101 cgcgcatttg ccctggctgc cagggttgga tctcacggct aaggttggca atgaccagca
    14161 aggtttgccc cttccattca cggcgatagc accacaatac agggctgttt ggcagcagat
    14221 cctggtaatt gccccatgtc aggatggctt cctgcttacg cagtgcgatt aacttttggt
    14281 aggtgtaaaa caccgaggaa tcatcggcca gcgcggcttc tacgttgatt tgttgatagt
    14341 tatcacccag gccaatccac ggttcgccag ccgtaaatcc ggcattatcg ccgttgctcc
    14401 attgcatggg cgtgcggctg ttgtcacggg atttactggc gaggattgcc aataactcgt
    14461 cggcatcacg accatcgttg cgcagctcgg caaacatatt gaggctctct acgtcgcgat
    14521 agtcagtaat gcgcgtgaaa tgcgggttgg tcatgccaat ctcttcgccc tggtagatat
    14581 acggcgttcc ctgcatgcca tgcagcacca tcgccagcat ttttgccgca ggcacgcggt
    14641 attcaccttc atcaccaaag cgagaaacaa tgcgcggctg atcgtggtta caccagaaca
    14701 aggcattcca tgctacgttg tgcattcctt gttgccagtg gcggaacaat gttttcaacg
    14761 ccacaaagtc aggtttagcc agcgtccatt tttcaccacc gggataatcg accttcaggt
    14821 gatgaaaatt aaaggtcatc gacaattcac tgcctgtcag tgccgcgtat cgctggcaat
    14881 gctcaaggct ggtggaggac atttcaccta cggtcattaa cccgcgtggc gtaaacacat
    14941 cgcggttcat ctcgtgcaaa aactcgtgtg ctcgtggccc gtcggtgtag aagcgacgcc
    15001 cgtcgccatc caggtcatca gggaaacgcg ggtctttgga gatcagattc accacatcca
    15061 ggcgcaaccc gtcgacccca cgatcggccc agaactcaca gacttttttc agcactgcgc
    15121 gtaccgctgg attctcccag ttgagatccg cctgttctgg tgcaaagaga tgcaaatagt
    15181 actgttcgct ttccgcatgc cagcgccacg cactaccgcc aaattttgaa cgccagttgt
    15241 tcggtggcgt ttctggttct ccatcgcgcc agatataaaa ctggcggtaa gggctttctt
    15301 tgttcagcgc ctcgcgaaac caggcatgtt gggtagaggt atggttaaac accatatcga
    15361 gaatgatacg aatcccgcgc gattttgcct gcgtcaccag ttcgtcaaaa tcgtccagcg
    15421 tgccgtaggt gggatcaatc gccgtatagt tcgctacgtc gtaaccgtta tcgacctggg
    15481 gagagacata aaagggggtt agccagatgg catcaacgcc cagtttatgc agatagtcca
    15541 ggcgttggat aacgccacgt aaatcgccgg taccgctacc cgtggtgtcc tgaaaactct
    15601 ttggataaat ctggtagata acgccgtttt gccaccagtg gggaggatta gtcattacgt
    15661 tattcctgca aatgcgaggg ggcgcaattg cgccccgaag aaaattaaac aatgtccagc
    15721 gtgcccaggc ggtatttccg ctgatagata aacgaggtga gtacaatcgg gatgatgatg
    15781 gcgatagcca ttgccagcgc aaacacctgc cagtagttcg gttgaatcga gagaattccc
    15841 ggcaggccgc ctacgccgat gccattcgcc ataacgccgt tcaggccgca tagcaatcct
    15901 gccagaccag aaccaatcat cgcgcacagc atcgggaagc gatatttcag gttgatgccg
    15961 tacattgcag gctcagtgac cccaagccag gcggagatcg ccgcaggcac ggagatctcg
    16021 cgttcattgt gcttgcggct ggaaatgata atgccgatca cggcggagcc ctgagcgata
    16081 ttcgacagcg caatcagtgg ccacactggc gtaccaccca tgctttgaat catctgcaaa
    16141 tcaatagcaa gcgtggtctg gtgtaccccg gtgatcacca gcggggcgta caggaagcca
    16201 aacaatgccg cgccaatcgg agcaaagctg ccggtcatca ggtgacgtac cgcaaaggca
    16261 acgccatcgc caatcatgcg accaaacgga ccaatcagcg catgggcgag gaacaccgcg
    16321 aggatcagcg aacagacggg caccaccacc agatagaggt aatctggcac gatgcgttta
    16381 aggcgagttt caataacgcc cagcgccagc ccggctaaca gtgccgggat cacctgcgcc
    16441 tgatagccca ctttggcgat gctgaacatg ccaaagtccc acacttccgg cagctgctgc
    16501 ccgagcagat aagcgttcat cagttggggg gaaaccagtg tcacgccaag cacgatacca
    16561 aggatcggtg tgccgcccat ttttttcacc gctgaccagc aaatcccgac cggcaggtag
    16621 aagaagatcg cttcaccgat caaccacaga aaatcgtaga tcgtttgcag ggaagggtac
    16681 atttgcgcca gcgtttgacc gttgctcatg ggcaaatcgc cgatcacatt gcgaaaaccg
    16741 aggatcaaac cgccgctaat caacgcgggc agcaacggga agaaggtctc tgcgaaatga
    16801 gagatcaact gctcatgcca tttcatattc tgccgggcgg ctttttttac ctgctcttta
    16861 tcaacctgcg cctgtccggt tgacgctatc agtgcttgat agtagtcacc cacgttggtg
    16921 ccaatcacca cctgaaattg cctggcattg gtgaaacagc ctttcaccat ggggagttgc
    16981 tcaatttctt tcggtctggc attggccggt tggttgagga caaagcgtag gcgagtaata
    17041 cagtggctca ccgtcgcaat attgccgcgc ccgccgacca gttcaatcaa ccgatcgata
    17101 tccgtttggt ttattttgct catcataaag ccccatggca gatgacattt ttggttggct
    17161 gcagaatata ttgcgcggaa aaatttaaaa acgggaacgt tccctaaacg cagcgaagat
    17221 cacaatttat cgttcaggaa acgatctgga cagggtggcg gggatgatga tttgttgcgg
    17281 ttcgctgcgc ccggttacct gtgcgatcaa ctggcaggcc gcctggcgtc cagcttcggc
    17341 gtaaccggga tctacggtga cgatctccgg atggaggaat ttcattaacg gcgtattacc
    17401 gacgctcgcc agttgcaagg tgtcgatgcg ttgctcttgc aggtatttac ttgcgccaag
    17461 tgccagcgtg tcggttgcgc acagtaaggc ggtagtttca ggcgtaatca cttttgctac
    17521 gttctcatag ccttgcttca tagcaaggcc tggcagagcg gcaacgggat gcagtttatg
    17581 cgctttgcag aacgccaggt aggcttcgtg acgtcgctta ccggtcgtca cgtcactgtg
    17641 cggcacgccg agataactga tattacgatg cccctggtca tacagccgtt gcatcaggat
    17701 cttgattgcc ccttcgtcgt cataacagac cgaagcaaag ccttttgcgt cacgcgccag
    17761 cagaaccagc gatgactgcc agtgggctaa catttcttct gttatgccgg taaaaccgaa
    17821 cagcactacg ccgtcgatat tacgccgttt cagcaccccc aaatgttcgg caactaattg
    17881 cggggaaaac tgactttcca tcatgattgg gtcgtaacct tgttcataga actctggcag
    17941 catggtttga acggcgagat tttctgacaa cgaatccagg cgggtaacaa tgatggcgac
    18001 cactttatcg ctttgcccac gcatagcgcg cgcagagcgg gaaggggaaa atccatgctg
    18061 attcatcact gcttcaacac gctcgcgggt gcgctggctc acgccgcttt cgttattcag
    18121 cacccgggaa actgtagatt tccccacgcc gcttaagcgc gcgatgtctt tgatggtcag
    18181 ccgattttgc atcctgttgt cctgtaacgt gttgtttaat tatttgagcc taactttacc
    18241 cgtgcattca gcaatgggta aagtctggtt tatcgttggt ttagttgcca gcaggtatta
    18301 tatcgccata gatgctacga atattattgg attctcctta ttatttgcgg cgcttttttc
    18361 acttaccgga ggttatatgg aacctgatcc cacgcctctc cctcgacgga gattaaaact
    18421 tttccggtaa gcccgtcttt tcacggcgtt accggatgcg taaggccgtg acgttttaac
    18481 gtccctgctc agctttatta ccttcaggta aggcttcgcc acgcctgaag acatttctgt
    18541 actgtttcag acagtgcgga gggactcctt atgtttaaag aaatttttac ccggctcatt
    18601 cgccatttac cttcccgtct ggttcatcgt gatccattgc ctggcgcgca gcagacagtg
    18661 aatacggtgg tcccgccgtc cttaagtgcg cattgcctga aaatggcggt gatgcccgaa
    18721 gaagaattgt ggaaaacgtt cgacacccat ccggaagggt taaatcaggc ggaagtggaa
    18781 tctgcccgcg aacaacatgg tgaaaataaa ttacccgcac aacaactgtc gccgtggtgg
    18841 gtacatttat gggtctgcta tcgcaacccc tttaatattt tactcaccat tctcggcgct
    18901 atttcttacg ccacggaaga tttatttgct gcgggcgtta tcgcgctaat ggtcgctatt
    18961 tctacgttgc tgaactttat tcaggaagca cgttccacta aagcggcaga tgccctgaaa
    19021 gcgatggtca gcaataccgc gacggtgctg cgcgtaatta acgacaaagg cgaaaatggc
    19081 tggctggaga tcccgatcga ccagctggtg cccggcgata ttataaaact ggcggcgggg
    19141 gatatgatcc cggcagattt acgtatcttg caagcgcggg atctgttcgt cgctcaggcg
    19201 tcgttaaccg gtgagtctct gcccgtggaa aaagccgcta ccactcgcca gccggagcac
    19261 agcaatccgc tggagtgcga cacgctgtgt tttatgggca ccaccgtggt gagcggcacg
    19321 gcacaagcaa tggtgattgc tacaggtgcc aatacctggt ttggtcaact ggcggggcgt
    19381 gttagtgagc aggaaagcga gccgaatgcc tttcagcaag ggatcagccg cgtcagtatg
    19441 ctgctgattc gctttatgct ggtgatggca ccggtggtgc tgttaatcaa tggctacacc
    19501 aaaggcgact ggtgggaagc ggcgctgttt gcgctttcgg tcgcggtagg cctaacgccg
    19561 gaaatgttgc cgatgattgt tacctcgacg ctggcgcgcg gcgcagtaaa gctgtcgaaa
    19621 cagaaagtga tcgtcaaaca tctggatgct attcagaact ttggcgcaat ggatattctg
    19681 tgcactgata aaaccggcac cctgacgcag gataaaattg tgctggagaa tcataccgat
    19741 atctccggta aaaccagcga acgcgtgctg catagcgcgt ggttgaacag tcattatcag
    19801 accggactta aaaacctgct cgatacagcg gtgctcgaag gtacggatga agagtcagcg
    19861 cgctcgctgg ccagtcgttg gcaaaaaatc gatgagattc cgtttgattt cgagcgtcgc
    19921 cggatgtcgg tggtagtggc agaaaatacc gagcaccatc agctggtttg caaaggtgca
    19981 ttgcaggaaa tcctcaatgt gtgttcgcag gtgcgtcaca atggcgagat tgtgccgctc
    20041 gatgacatca tgctgcgtaa gattaagcgg gttactgata cgctgaatcg tcaggggctg
    20101 cgcgtggttg cggtggcgac gaaatacctg ccagcgcgtg aaggagatta ccagcgggcg
    20161 gatgaatccg acctgatcct cgaaggatat attgcttttc ttgatccgcc aaaagagaca
    20221 accgctccgg cactgaaggc attaaaagcg agtggaatta ccgtaaaaat ccttactggc
    20281 gacagtgagt tagttgcagc gaaagtgtgc catgaagtgg ggctggacgc gggagaggtg
    20341 gtcattggta gtgatattga aacgctatct gacgacgaac tggcaaatct tgcacagcgc
    20401 accacgctgt ttgcccgcct gacgccgatg cataaagaac gcattgtgac cttactgaag
    20461 cgcgaagggc atgtggttgg ctttatgggc gatggtatta atgatgcgcc cgccttacgc
    20521 gcggcggata tcggcatttc tgtggacggc gcggtagata ttgcccgtga agcggctgat
    20581 atcatcctgc tggaaaaaag cctgatggtg ctggaagagg gggttattga gggacgtcgc
    20641 actttcgcca acatgctgaa atacatcaaa atgacggcga gttctaactt tggtaatgtg
    20701 ttcagcgtgc tggtggcgag tgctttcttg cccttcctgc cgatgttgcc gttacacttg
    20761 cttattcaga acctgctgta cgatgtgtca caggtggcga tcccgtttga taacgtcgac
    20821 gacgagcaaa ttcaaaagcc gcagcgttgg aatccggcgg atctggggcg ctttatgatc
    20881 ttcttcggac cgatcagctc gatcttcgat attctgacgt tttgcctgat gtggtgggta
    20941 ttccatgcca acacgccgga aacgcaaacg ctgttccagt cgggatggtt tgtggtgggc
    21001 ttactgtcgc aaacgctgat tgtgcatatg atccgcaccc gccgtgtgcc gtttattcag
    21061 agctgtgcat cgtggccgtt aatgatcatg accgtgatcg tgatgattgt cgggatcgca
    21121 ttgccgtttt caccgctggc cagttatctg caattacagg cgttgccgtt aagctatttc
    21181 ccgtggctgg ttgcgattct ggcagggtat atgacgttaa cccagttggt gaaaggattt
    21241 tatagccgtc gttacggctg gcagtaaaga ataaactggg catggtggcc cggattcaga
    21301 ttgcagacaa agtgagtttt gttcatgccg gatgcggcgt aaacgcctaa tccggcctac
    21361 aaattcaatc aattgcagga atcacgtagg cctgataagc gtagcgcatc aggcgatttt
    21421 gcgtttgtca tcagacttaa tccgggcatg atagcccgga tttccatcaa gattagcgac
    21481 gaatagcgat cgcttcgatc tcaatcttta cgtctttcgg cagacgggca acttcaacgc
    21541 aagaacgtgc cgggaaggtg gcgttgtgtt cggtgaagaa ggtttcgtaa gtggcgttta
    21601 cggttgcgaa gtcgttcaga tcttttacaa acacggtagt tttaacgatg tcgcccactt
    21661 tcagaccagc ggcttcgacg atcgctttta cgttatccag cgactgacgt gcctgtgcag
    21721 cgacgtctgc cggtacttcg cccgttttcg gatttaccgg gatctgaccg gaggtgatga
    21781 tcatatttcc cagatcaacg ccctgtacgt aaggaccgat agctgctggt gcattttccg
    21841 tcgcgatagt tttgctcatg atttctcctt tattacagcg gtaaaaagtc tggctcatta
    21901 tagggagcca gacttttatt accaaccgca attaattggc cagcaccaca ttatgggaaa
    21961 actctttttc acagtatttg catttgagcg cgatgtcatt ggcgcgtttt cgcacggcaa
    22021 agctggatga aaccggttcg gcatggctga tacagttgct gttcgggcag accaacacat
    22081 tgtcgatgcg ctctggcaga ctggggcgcg atttacccac cacttcatag ttgtcgatac
    22141 ggttaaccgt ggcttgcggc gcatacaatg ccagttgatc tacctgatct tcactcaaaa
    22201 aggtattttc gattttgatc agatctttgc ggcccatctc gccagaaggc aggttcagac
    22261 caatggtgat gcgctgatcc gtttcggtca gcttgaacag actcaacagc ttaaaaccga
    22321 tctgggcggg gatatggtca attaccgtgc cgcgtttaat agcttcaacc tgcaatttat
    22381 tatcgtgtgt catctctatt tccccttaca gtaccagatc gcgattcaga accagtgcca
    22441 gtaacgcctg gcgagcgaaa atcccgttgc ctgcctgctg gaagtaccag gcgtgtggcg
    22501 ttttatcaac atccgtcgca atttcatcaa cacgcggcag cggatgcagt actttcatat
    22561 tggctttggc gttgtggaga tcgctggcgc gaagaacaaa ctgcgctttc acgttggcgt
    22621 attcggatgg gtccaggcgc tctttttgca cgcgggtcat atacaggatg tctacttccg
    22681 ccatcacttc ttcaatagag ctgtgcagac tccatgcgat ccctttttca tcgagcatat
    22741 ccagaatgta ttgcggcatt gccagcgcgt ccggcgcgat gaagtaaaaa cggttgccgt
    22801 cgaacttcgc taacgcctga gtcagggagt gaacggtgcg gccatatttc aggtcaccaa
    22861 ccattgcgac gtggagattg tccagacgcc cctgggtttc ctgaatagtg aataagtcca
    22921 gcaaggtttg cgtcggatgt tggttggagc catcaccggc attcagtacc ggtacattgc
    22981 cggaaaactc ggtggccagg cgcgccgcac cttcctgcgg atgacgcatc actatcgcat
    23041 cgacgtaagt gctgataacc gaaatggtat cggccagcgt ttcgcccttt ttacccagtg
    23101 atgtattggc gctgtcggag aagcccacca cgctggcacc caggcggtgc atggaagttt
    23161 cgaaagagag gcgggtacgg gtagaggctt cgaagaagca gctggcaatg actttgtgct
    23221 tcaacagctc tggttgcggg tttgctttca gttttgccgc tgttgccagc accagattaa
    23281 ggtcatcgcg actaaggtcg tttatggaaa tgatatgttt ctgatatagc ggattagcca
    23341 tcttttatct cctgacgcct gggcaaaaaa aagcccctcg attgaggggc tgggaatggg
    23401 tgatcaacgg gaagaaaaac ggcaggccag cgtctttttt cagacgcggt aagacaaaat
    23461 gtcgaacaca ctgaaccata catcctcccg gcaaattgtc cggcattata ctcatcgtca
    23521 gaagcggtgc aagcaattga tgcaatattt tgtcagcgca aacggtttat ttgaatttaa
    23581 agttaaaata tatgcatttt tatttactgt gattctgggg ggaactgtcg ttcggtatca
    23641 ataattgcag acatttcctg cttttttctt tttcactttc acgcaatcag atatgcattt
    23701 tattccattc atccgactta tagggcggag tgtgaaaacg aacggctagc actattgctt
    23761 agagctcagg gatgcgcgct atcactttaa tttcaaaatc aaagcctgcc agccatgtaa
    23821 cgcccaccgc cgtccaggtt ggataagatg gcgcgctaaa tatttcattt ttcaccgtca
    23881 tgatgtcttc aaattgtttt tctggatcgg tatgaaagct cgtaacatca atgatatcgt
    23941 caaaagtgca tcccgcagct gccagggtcg catgcaaatt atcaaaggcc agtctgactt
    24001 gttgctgaaa atcgggttct ggtgttccgt cctctcgact tcctacttgc ccggaaacaa
    24061 acagcaaatc gccggaacga atagccgcag aataacgatg ctcagcatac agtgaatgtc
    24121 gtccagcagg gaaaacagcg gttctttcta ccatttggtt atcctcaaga tttacgacat
    24181 gaacagaaga tttatattta ccaggagccg cttttagcgg acgacgtgag taaacaaaac
    24241 ccagacatca tgggaaatgg ctgggcttaa ttgagcgtag tcggttatgc gccaaacgcg
    24301 ccatcaatgg tatgcatcgc gccggtaaca aaactggctt ctggccctgc taaccatgcg
    24361 accataccag cgacctcttc cggttgccca tgtcttttga tggccataaa actatgcgac
    24421 atatcgcgca ttggcccgtt ggcgggatta gcgtcggtat caattggccc tggctggacg
    24481 acgttgatgg tgatcccacg cggtccgaaa tcacgggcca gcccgcgcgc catgccttgc
    24541 agggcagatt tactggcggc ataagcagcc atgcctgcaa caggcatacg atcgccattc
    24601 acggagccga tgattaagat gcgtccgcct tcgggcatct gccgggcggc ttcaacagag
    24661 gcatggtaag gagcatgaat attgattttg aaaaggcgat caatatcgtc ggcatttaat
    24721 tccagggcat cgccaaagac gccaatacct gcatttacca ccaggatatc caatgcgccg
    24781 ctcttacgaa cgacatcaat aacagcgtct ttgtcagcac tatctgtgaa tactgctgtc
    24841 gctccagtct cttgtgccag gcgttcagcg gcatctttcg accccgcata ggtgaatcgt
    24901 acattggccc catcggtgac gaaacgacgt acgatagcgg caccgattcc acggctacca
    24961 ccgaggatga gaactgtctt acctgtaaaa gcgcccataa ggactccttg atttattatg
    25021 taacatgcat tacaaaactg ttttaacttt ctgtcaacaa gttttgtaat ggtcactaaa
    25081 aaacaatctc gcgttccagg tcgtcccaga cgtttcgctc ctgagcaggc agtctctgcg
    25141 gcaaaagtgc tttttcacca aaaaggtttc gatgctgtca gtgttgctga agttactgat
    25201 tatcttggta ttaacccccc gagcctctac gcggcttttg gcaataaggc tgggttattt
    25261 agccgtgtac tcaatgaata tgtcggtacg gaagctattc cgcttgccga tattcttcgt
    25321 gatgatcgtc cggtagggga gtgcctggct gaggtattaa aagaagcggc gcgccgatat
    25381 agccaaaacg gcggctgcgc tggctgtatg gttcttgaag gcattcatag tcatgatcca
    25441 caagcgcgtg atattgccgt tcaatattat cacgccgcag aaacgaccat ttatgactat
    25501 atcgccaggc ggcatccaca aagcgcacaa tgtgtgactg attttatgag taccgtgatg
    25561 tcagggcttt ctgcgaaggc acgggagggg cactccatcg aacaactctg tgcaacagct
    25621 gcactggcgg gggaagcgat aaaaactatt ctcaaggagt gatgcgggcc ttgacccgaa
    25681 aggcgggaac gcgcctgccg ataagttgtg ataagacaat aattcacgcg ttaaggctag
    25741 cggaattgat catcttttcg tataacgatg gaaatgaaac gttgttttaa ttaaggagtg
    25801 gaaaagatga tcatcggaaa tattcataat cttcagccgt ggctacccca ggagttacgc
    25861 caggcgattg agcatatcaa agcacacgtt acggcagaaa cgccaaaggg caagcacgat
    25921 atcgaaggca atcgtctgtt ttatcttatc tcggaagata tgaccgagcc gcacgaagct
    25981 cgccgtgcgg agtaccatgc ccgctatctc gacattcaga ttgtgttaaa aggtcaggaa
    26041 ggcatgacct tcagcacgca acctgcaggc accccggata ccgactggtt agctgataaa
    26101 gacatcgcat ttttgccgga aggcgttgat gagaaaacag ttatccttaa tgaaggtgat
    26161 tttgttgtgt tttatccggg ggaagtgcat aaaccgctgt gcgcagtggg cgcgccagcc
    26221 cgggttcgca aagcagtagt gaagatgctg atggcgtgat gacttttcgc cgtaaataac
    26281 tccaggttta cggcgagttt gtgaaaagag cgttttttga tatttttttg tgagtaaaat
    26341 ttgtaatgct tagacgttct tattaactca aggagttcgt catgagcaaa atatcagatt
    26401 tgaattattc tcaacacatt acattagccg acaattttaa acaaaaaagt gaagttttaa
    26461 atacctggcg tgttggaatg aataattttg cccgtaatgc cgaggggcag gataacacaa
    26521 gaaatatcct tgatcctaag acatttttgg aatttttggt aaaaatattt actctaggtt
    26581 atgtggattt tagcaaacgc tccaacgaag cgggaagaaa tatgatggct catattgagt
    26641 cctcatctta tatcaaaaat aatgatggca gtgagataat aaagtttgtt atgaataatc
    26701 ctgaagggga acgagcggat ttatctaagg tggaaataga aatcacactt tccactttca
    26761 ctactatggg gactcgtcaa ggacatacgg ctattatatt tcaacaacct gatggttcga
    26821 ctaatcgtta tgaaggaaag tcctttgaaa gaaaagatga gagttcatta cacctgatta
    26881 ctaacaagat tctggcatgt taccaacgcg aagctaacaa ggaaatagct cgtctattaa
    26941 atatttcaca ggagttaaat aattctcagg acttgaataa ctcgcaggtg agttgtaaag
    27001 attcagttga ttctacgatt acggatttat tagaaaaacc attgaataat gcattattag
    27061 caataaggaa agaacatctg ctattaatgc cttatgtatg taatgaatcg atttcatact
    27121 tgcttggtga aaaagggata ctgaaagaaa tagatgacct taacgcagta aataattatc
    27181 ttcttaataa taagaaagct actgacaatg aaattaatga catcaaagtt aacctgtctc
    27241 atattcttat tgattcctta gatgatgcaa aagttaacct tacaccggtc atcgattctg
    27301 gagacttttt taaaatcccc atatattaat gatgtaagaa tactggattg gtgttttaat
    27361 aaaagaatgc aatattttgg tgattctgaa aagataaagt atgcatgctc cgtgataaat
    27421 catattgatt ttagccgcga tcaatctaaa gattttagct gcgatcaatc taaaattaaa
    27481 atagctgaga ccttattttt caatctcgat aaagaaccct ataaaaa
//
LOCUS       DAJTMC010000039        27443 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.039, whole genome shotgun sequence.
ACCESSION   DAJTMC010000039 DAJTMC010000000
VERSION     DAJTMC010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27443
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.039"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            435..3620
                     /gene="rne"
                     /locus_tag="OO130_001477"
     CDS             435..3620
                     /gene="rne"
                     /locus_tag="OO130_001477"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HCR5364937.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAAPVVAPAPKAAPATPAAPA
                     QPGLLSRFFGALKALFSGSEETKPTEQPAPKAEAKPERQQDRRKPRQNNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPHHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(3817..4770)
                     /gene="flgL"
                     /locus_tag="OO130_001478"
     CDS             complement(3817..4770)
                     /gene="flgL"
                     /locus_tag="OO130_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HCR5364938.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFAIQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEEKGKYVGGAESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKEIAAAALDKTNRGLKNSLNNVLAVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(4782..6425)
                     /gene="flgK"
                     /locus_tag="OO130_001479"
     CDS             complement(4782..6425)
                     /gene="flgK"
                     /locus_tag="OO130_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HCR5364939.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITMANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQDLDQTRNTLGQLALAFAEAFNTQHKAGFDANGDAGEDFFAIGKPA
                     VLQNTKNKGDVAIGATVTDASAVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASEKDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSATQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(6491..7432)
                     /gene="flgJ"
                     /locus_tag="OO130_001480"
                     /pseudo
     CDS             complement(6491..7432)
                     /gene="flgJ"
                     /locus_tag="OO130_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
     gene            complement(7432..8529)
                     /gene="flgI"
                     /locus_tag="OO130_001481"
     CDS             complement(7432..8529)
                     /gene="flgI"
                     /locus_tag="OO130_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HCR5364940.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVSGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSARLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(8541..9239)
                     /gene="flgH"
                     /locus_tag="OO130_001482"
     CDS             complement(8541..9239)
                     /gene="flgH"
                     /locus_tag="OO130_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HCR5364941.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVVDARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(9292..10074)
                     /gene="flgG"
                     /locus_tag="OO130_001483"
     CDS             complement(9292..10074)
                     /gene="flgG"
                     /locus_tag="OO130_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HCR5364942.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMQQKLTQL"
     gene            complement(10212..10967)
                     /gene="flgF"
                     /locus_tag="OO130_001484"
     CDS             complement(10212..10967)
                     /gene="flgF"
                     /locus_tag="OO130_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HCR5364943.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKIDYTSRPLDVALQQDGWLAVQTADGSEGYTRN
                     GSIQVDPTGQLTIQGHQVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAETQATRGPVLQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(10987..12195)
                     /gene="flgE"
                     /locus_tag="OO130_001485"
     CDS             complement(10987..12195)
                     /gene="flgE"
                     /locus_tag="OO130_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HCR5364944.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     NSIAKTATTLEFNANGTLVDGAMANNIATGAINGAEPATFSLSFLNSMQQNTGANNIV
                     ATPQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWS
                     ATQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQD
                     QILNTLVNLR"
     gene            complement(12220..12915)
                     /gene="flgD"
                     /locus_tag="OO130_001486"
     CDS             complement(12220..12915)
                     /gene="flgD"
                     /locus_tag="OO130_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HCR5364945.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTMLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPNGSYNVAITASNGGTQLVAQPLQFALVQGVIRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(12927..13331)
                     /gene="flgC"
                     /locus_tag="OO130_001487"
     CDS             complement(12927..13331)
                     /gene="flgC"
                     /locus_tag="OO130_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HCR5364946.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            complement(13335..13664)
                     /gene="flgB"
                     /locus_tag="OO130_001488"
     CDS             complement(13335..13664)
                     /gene="flgB"
                     /locus_tag="OO130_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HCR5364947.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTLGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYQMSLSALSGQIKGMM
                     NVLQSGN"
     gene            13820..14479
                     /gene="flgA"
                     /locus_tag="OO130_001489"
     CDS             13820..14479
                     /gene="flgA"
                     /locus_tag="OO130_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HCR5364948.1"
                     /translation="MLAIKRSVAIIAILFSPLSAASNLTSQLHTFFSAQLVGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAISLRDLSPDQPIQLTHFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARMRMVSGLVVSGVVDADGNILIN
                     L"
     gene            14555..14878
                     /gene="flgM"
                     /locus_tag="OO130_001490"
     CDS             14555..14878
                     /gene="flgM"
                     /locus_tag="OO130_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
                     /protein_id="HCR5364949.1"
                     /translation="MSIDRTSPLNPVSTVQPRETTDAPVTNTRAAKTTDSTSTSTSTS
                     TSTSTSVTLSDAQAKLMQPGSSDINLERVEALKLAIRNGELKMDIGKIADALINEAQQ
                     DLQSN"
     gene            14883..15299
                     /gene="flgN"
                     /locus_tag="OO130_001491"
     CDS             14883..15299
                     /gene="flgN"
                     /locus_tag="OO130_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HCR5364950.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGGKKISI"
     gene            complement(15339..16874)
                     /gene="murJ"
                     /locus_tag="OO130_001492"
     CDS             complement(15339..16874)
                     /gene="murJ"
                     /locus_tag="OO130_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HCR5364951.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(17041..17907)
                     /locus_tag="OO130_001493"
     CDS             complement(17041..17907)
                     /locus_tag="OO130_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR5364952.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWMLQGAWSPTRAKALP
                     ICESWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVHKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGE"
     gene            complement(17909..18556)
                     /locus_tag="OO130_001494"
     CDS             complement(17909..18556)
                     /locus_tag="OO130_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HCR5364953.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAELESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTDMSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(18567..19151)
                     /gene="rimJ"
                     /locus_tag="OO130_001495"
     CDS             complement(18567..19151)
                     /gene="rimJ"
                     /locus_tag="OO130_001495"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HCR5364954.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            19387..20595
                     /gene="mdtH"
                     /locus_tag="OO130_001496"
     CDS             19387..20595
                     /gene="mdtH"
                     /locus_tag="OO130_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HCR5364955.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            20659..21306
                     /gene="grxB"
                     /locus_tag="OO130_001497"
     CDS             20659..21306
                     /gene="grxB"
                     /locus_tag="OO130_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HCR5364956.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            21440..22000
                     /locus_tag="OO130_001498"
     CDS             21440..22000
                     /locus_tag="OO130_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5364957.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            22106..23152
                     /gene="pyrC"
                     /locus_tag="OO130_001499"
     CDS             22106..23152
                     /gene="pyrC"
                     /locus_tag="OO130_001499"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HCR5364958.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHNFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREAHFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            23226..23471
                     /gene="dinI"
                     /locus_tag="OO130_001500"
     CDS             23226..23471
                     /gene="dinI"
                     /locus_tag="OO130_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HCR5364959.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGVTKEDKQRISEILQETWESADDWFVSE"
     gene            23761..24015
                     /gene="bssS"
                     /locus_tag="OO130_001501"
     CDS             23761..24015
                     /gene="bssS"
                     /locus_tag="OO130_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HCR5364960.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            24130..25248
                     /gene="solA"
                     /locus_tag="OO130_001502"
     CDS             24130..25248
                     /gene="solA"
                     /locus_tag="OO130_001502"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HCR5364961.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCTGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
     gene            25296..25409
                     /locus_tag="OO130_001503"
     CDS             25296..25409
                     /locus_tag="OO130_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HCR5364962.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            25670..26236
                     /locus_tag="OO130_001504"
     CDS             25670..26236
                     /locus_tag="OO130_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HCR5364963.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFTLGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            26240..26815
                     /locus_tag="OO130_001505"
     CDS             26240..26815
                     /locus_tag="OO130_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HCR5364964.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGNDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(26857..>27443)
                     /locus_tag="OO130_001506"
     CDS             complement(26857..>27443)
                     /locus_tag="OO130_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HCR5364965.1"
                     /translation="NHYEYEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYC
                     TGGIRCEKASAWMKHNGFNKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGER
                     ISDEIIAHCHQCGAPCDSHTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPE
                     EQRRRRAGRENGNKIFNKSRGRLNTTLGIPDPTE"
BASE COUNT         6583 a         7308 c         6911 g         6641 t
ORIGIN      
        1 actgtgagta agcgggtgat aaatggtaaa agtcatcttg ctataacaag gcttgcagtg
       61 gaataatgaa gccgtttccg tgtccatcct tgttaaaaca agaaatttta cggaataacc
      121 cattttgccc gaccgatcat ccacgcagca atggcgtaag acgtattgat ctttcaggca
      181 gttagcgggc tgcgggttgc agtccttacc ggtagatgga aatatttctg gagagtaata
      241 cccagtctgt ttctttgata attgcgctgt ttttccgcat gaaaaacggg caaccgacac
      301 tctgcgcctc tttgagctga cgataaccgt gaggttggcg acgcgactag acacgaggcc
      361 atcggttcac acccggaaag gcgttacttt gcccgcagct tagtcgtcaa tgtaagaata
      421 atgagtaagt tacgatgaaa agaatgttaa tcaacgcaac tcagcaggaa gagttgcgcg
      481 ttgcccttgt agatgggcag cgtctgtatg acctggatat cgaaagtcca gggcacgagc
      541 agaaaaaggc aaacatctac aaaggtaaaa tcacccgcat tgaaccgagt ctggaagctg
      601 cttttgttga ttacggcgct gaacgtcacg gtttcctccc actaaaagaa attgcccgcg
      661 aatatttccc tgctaactac agtgctcatg gtcgtcccaa cattaaagat gtgttgcgtg
      721 aaggtcagga agtcattgtt cagatcgata aagaagagcg cggcaacaaa ggcgcggcat
      781 taaccacctt tatcagtctg gcgggtagct atctggttct gatgccgaac aacccgcgcg
      841 cgggtggcat ttctcgccgt atcgaaggcg acgaccgtac cgaattaaaa gaagcactgg
      901 caagccttga actgccggaa ggcatggggc ttatcgtgcg caccgctggc gtcggcaaat
      961 ctgctgaggc gctgcaatgg gatttaagct tccgtctgaa acactgggaa gccatcaaaa
     1021 aagccgctga aagccgcccg gccccgttcc tgattcatca ggagagcaac gtaatcgttc
     1081 gcgctttccg cgattactta cgccaggaca tcggcgaaat ccttatcgat aacccgaaag
     1141 tgctcgaact ggcacgtcag catatcgctg cattaggtcg cccggatttc agcagcaaaa
     1201 tcaaactgta caccggcgag atcccgctgt tcagccacta ccagatcgag tcgcagatcg
     1261 agtccgcctt ccagcgtgaa gtccgtctgc cgtccggtgg ttcgattgtt atcgacagta
     1321 ccgaagcgtt aactgccatc gacatcaact ccgcacgcgc gacccgcggc ggcgatatcg
     1381 aagaaaccgc gtttaacacc aacctcgaag ctgccgatga gattgctcgt cagctgcgcc
     1441 tgcgtgacct cggcggcctg attgttatcg acttcatcga catgacgcct gtacgccacc
     1501 agcgtgcggt agaaaaccgt ctgcgtgaag cggtgcgtca ggaccgtgcg cgtattcaaa
     1561 tcagccatat ttctcgcttt ggcctgctgg aaatgtcccg tcagcgcctg agcccatcac
     1621 tgggtgaatc cagtcatcac gtttgtccgc gttgttctgg tactggcacc gtgcgtgaca
     1681 acgaatcgct gtcgctctct attctgcgtc tgatcgaaga agaagcgctg aaagagaaca
     1741 cccaggaagt tcacgccatt gttcctgtac caatcgcttc ttacctgctg aatgaaaaac
     1801 gttctgcggt aaatgccatt gaaactcgtc aggacggtgt gcgctgcgtg attgtgccaa
     1861 acgatcagat ggaaaccccg cactaccacg tgctgcgcgt gcgtaaaggg gaagaaaccc
     1921 caaccttaag ctacatgctg ccgaagctgc atgaagaagc gatggcgctg ccgtctgaag
     1981 aagagttcgc tgaacgtaag cgtccggaac aacctgcgct ggcaaccttt gccatgccgg
     2041 atgtgccgcc agcgccaacg ccagctgaac ctgccgcgcc tgttgtagct ccagcaccga
     2101 aagctgcacc ggcaacacca gcagctcctg cacaacctgg gctgttgagc cgcttcttcg
     2161 gcgcactgaa agcgctgttc agcggtagtg aagaaaccaa accgaccgag caaccagcac
     2221 cgaaagcaga agcgaaaccg gaacgtcaac aggatcgtcg caagcctcgt cagaacaacc
     2281 gccgtgaccg taatgagcgc cgcgacaccc gtagtgaacg tactgaaggc agcgataatc
     2341 gcgaagaaaa ccgtcgtaat cgtcgccagg cacagcagca gactgccgag acgcgtgaga
     2401 gccgtcagca agctgaggta acggaaaaag cacgtaccac cgacgagcag caagcgccgc
     2461 gtcgtgaacg tagccgccgc cgtaacgatg ataaacgtca ggcgcaacaa gaagcgaagg
     2521 cgctgaatgt tgaagagcaa tctgttcagg aaaccgaaca ggaagaacgt gtacgtccgg
     2581 ttcagccgcg tcgtaaacag cgtcagctca atcagaaagt gcgttacgag caaagcgtag
     2641 ccgaagaagc ggtagtcgca ccggtggttg aagaaactgc cgctgccgaa ccaattgttc
     2701 aggaagctcc agctccacgc acagaactgg tgaaagtccc gctgccagtc gtagcgcaaa
     2761 ctgcaccaga acagcaagaa gagaacaatg ctgataaccg tgacaacggt ggcatgccgc
     2821 gtcgttctcg ccgctcgcct cgtcacctgc gcgtgagtgg tcagcgtcgt cgtcgctatc
     2881 gtgacgagcg ttatccaacc cagtcgccaa tgccgttgac cgtagcgtgc gcgtctccgg
     2941 aactggcctc tggcaaagtc tggatccgct atccaattgt acgtccgcaa gatgtacaag
     3001 ttgaagagca gcgcgaacag gaagaagtac aagtgcagcc gatggtgact gaggtccctg
     3061 tcgccgccgc tgtcgaaccg gttgttagcg cgccagttgt tgaagaaatg gctgaagtcg
     3121 tagaagcccc cgtaccggtt gccgaaccgc aaccggaagt ggttgaaacg acgcatcctg
     3181 aagtgattgc cgccgcggta actgaacagc cgcaggtgat taccgagtct gatgttgccg
     3241 tagcccagga agttgcagaa cacgcagaac cggtggttga accgcaggaa gagacggcag
     3301 atattgaaga agttgccgaa actgctgagg ttgtggttgc agaacctgaa gttgttgctc
     3361 aacctgccgc gccagtcgtc gctgaagtcg cagcagaagt tgaaacggta gccgcggttg
     3421 aaccagagat caccgttgag cataaccacg ctaccgcgcc aatgacgcgc gctccggcac
     3481 cggaatatgt tccggaggca ccgcatcaca gtgactggca gcgccctact tttgccttcg
     3541 aaggtaaagg tgccgcaggt ggtcatacgg caacgcatca tgcctctgcc gctcctgcgc
     3601 gtccgcaacc tgttgagtaa taattagctc aaagtaatca agccctggta actgccaggg
     3661 cttttttatt tcatgtttga atcaacatat taaaattcat caaatggcat ccttgctaac
     3721 caacaatgca aaataggcaa aaaaaatgcc cgccccggcg atccctgagc gggcagacaa
     3781 aacataccca gtttcatgat atgttttaaa gcgagcttat ttgttgagct ggaagagcga
     3841 caatccctgc atatcggtaa atgctttata cgatgcctgc aatgccgttt gctgcatgat
     3901 gtaagatgaa atagttgcat tccagtccac atcaaccaga tcgctcatct gctgcgtttg
     3961 ccctaaagcg cgatcgctac ctaaggaatc cagcgactcc agttcgttca gctgcgtgcc
     4021 taattccgcg cgaacggcaa ggacattatt cagcgagttt ttcagtccac ggttggtttt
     4081 atccaatgcc gcagcggcga tttctttatc cgcttcgcta tccgcgaccg gcgttttcag
     4141 ggctgcgatg gcgctatcca gcatggcaaa aagattggtt tcagaagcgc taccgtctgg
     4201 ctccgcaact gcgttgctgg taatactgtc gaaaattttg tcacccgtgt gccctatcac
     4261 catcgaacgc gaagcatcga cctgttgctt aatactttct gctccaccga cgtatttccc
     4321 tttctcttcg ctaaacggcg cagtctctgt tttataaccg gcaaaaatgt agcgcccgtt
     4381 accgtcagtg gtgttcgcca gattcagcaa ctggtcacga agcccctgaa tatccgtagc
     4441 cagcgaggcc cggtcatcgt cactcaaggt gccattgctg gcgtagacaa ttttttcctg
     4501 agcattctgg atagcagtgg tgacctggct aagtacactc tcttccagtg acactttttg
     4561 aatggcgaaa gtacgcgcca gcgtgtactg gctgttttgc gcctgtgcct gggagagaac
     4621 tacggcttgt gatgctgcaa tgggatcgtc agaagggtta acgactcgct tacctgtcga
     4681 catctgctcg ccgtacttca tccattccgc ctgagaattg gtgatgccac gcatgttttg
     4741 ctggtacatc atctgggtac tgaaacgcat cttatctccc cttagcgaat gttaatcagc
     4801 gcatcaaaaa tcgcgtttgc cgtctgcaga acctgcgcat tcgccaggta atactgctga
     4861 aaacgttgca gatttccgta ctcttcgtcg agattgacgc cagagataga ctgctgctga
     4921 ttggaaagct gcgtcaccac attaccttgc gtggcgctac tggttttcaa cgtcgcggtt
     4981 ttattaccga tatcactcac taacgaggca taagcgtcgt taaaggattt cgcaccgccc
     5041 accgttttac tgttgctttg cagatccagc agggcctgac cgttgcggtt atcgctatca
     5101 cccgcatctt tttcgctcgc catcgctatt ttcgcttcgt cggtgattaa tacatccatg
     5161 ttgacgatgg cgtcacttac tggtttcagc gtgaagctgt cgttaacggc aggcgttcct
     5221 gtaaacgtca actccagacc atcaaatgcc actttaccgt tggcatccgg cgtcaccgta
     5281 aaagtggtat tgctggcaag gcgggtgacc tgccactgat tattatcgaa cgagattttg
     5341 taatctgtcg ccagtaccgc ggaggcatca gttaccgtgg caccgatcgc aacgtcacct
     5401 ttgtttttcg tgttttgcaa aaccgcgggc ttaccgatag caaagaaatc ttcaccagca
     5461 tcgccgttgg catcaaatcc ggctttgtgt tgggtgttga aagcctcggc aaatgccagc
     5521 gccagttgtc caagcgtatt acgcgtctgg tccagatcct gagaacggaa tgtcagaatg
     5581 ccgcccagcg acccggtatt cagtaatttc tccgggatct caatattgcc tgccgtccca
     5641 tcaacgtaag cgacagtcgt acgagaaggg tcggcgctgg aaggaactgc cgccagttgc
     5701 cgcgccgtac ttccctgaac cagtgagtaa ccattggcca tcgtgatgtt ataagtgccg
     5761 ccatcctgaa cgctgacttc tacaccaaca atctggttta attcgctcac cagttgatcg
     5821 cgttgatcca gcagattgtt aggtgacgcc cctgccccca cgcctgtcag gcgggagatt
     5881 tggtcgttca ggctggcgat ttgtttagcg taattgttga tctgatcaac gctggcacct
     5941 atcgcgatat tgacctgttt gtcctggtca cgaagatact gatcggtggt tttaaactga
     6001 ttcaccaacc cttctgattt cccaatcagc gcctggcgcg ctgccgggtc ttccgcgtta
     6061 ctcaccagcg tttgcaggct ggtgaagaaa tcctgcatct gtgttgccag cgaagaggta
     6121 ctggtggaga gcatattgtc gattttcgac atctgctcat agcgggcagt caggccgcta
     6181 ctttgcgtct gcgccgcacg taactggttg gtgataaacg catcatactc acgctgcaca
     6241 ccagaaacgt agacaccatt gccaacccag ccgccagcgc ccaacgtgct attggcctgc
     6301 gccataatag tggtttggcg ggtatatccg gcaacgttat agctggagat attattactt
     6361 gccgtattta acgccgcctg ggccgcgttc agtccgctca tggcgttatt aatcaagctg
     6421 gacatggagg ttccttataa gccttcaatt acagagtatt atcggcagcg acccgccgga
     6481 cttgagttat tcaaaacaga ttatcaatgt tcatactgta ggttttgctc accttgtcgc
     6541 ttatcgattt catctgctga atcatgttgg tgagtttgcg ggcatagtga ggatcggtgg
     6601 catagcccgc gtcctgtagg gcctgcgccc cctgctccgc actcgtggcg gtggtcacgg
     6661 cggcgtagcg cgggttacgc gttaacagcc caacgtaatc cgactatgct tccagatacg
     6721 agctgtagac ccgaaacttc gcttttactt tcttcgcttc gccgttttca tattcagtcg
     6781 tggtgatttc agtaactggc cctttccagt tgccagaggc tttgacacca aacaggttat
     6841 agctcggctc gccgttttcg cggcggattt gccgttgccc ccaaccagat tccagcgccg
     6901 cctgagcgag gatcaaatga tgtggcacac cgctttgctg gcttgccagt tgggcgggca
     6961 gcgagagttg cgcgaggaat gctttactgt cacccggcag cgaatcatcg tagttacgtg
     7021 gcacggcctt ttgcaccagc tgcgaaagcg cctgattttg ataacgcacc acagtttcga
     7081 gcgggaattt catcggtgct gctggcgtgg actcctctgg caatggttgt tctggcgtca
     7141 tctgtttaac catcatctct gcaagcccca gacctttgcc cgccgtcatc tgttgggcaa
     7201 tctgctggtc atacatactg gtatacaggc gagtgtgctc gctgctgaac aggccatctt
     7261 ttggtaaagc gtcgcgcatg cttttcaaca tcatctgcac gaacatccct tccacctgac
     7321 gggccaccgg acggatattt gccgccggat cttcgcccgc tttcgccttt agttcgttga
     7381 gtgattgcgc atcccaggcc gcacttgcca gtagtttgct atcgctgatc atcagatgat
     7441 ttccagtttt gcccgcagac atcccgcact ttgcattgat tgcagtatgg acatcagatc
     7501 catcggcgta gcgcccagcg cattgagcgc gcgcaccacg ttattgagcc tggcgctgga
     7561 acgtacgctt tgcagcgaac cgccgctctg gcgtaaatcg atctgcgttt gtggagtaac
     7621 cacagtctgt ccaccaccaa acggtgtatc tggctggctg acattggcct gacgattaac
     7681 tgttactgag agattcccct gcgctaccgc gcagctgtcg agggtcactt cgcgattcat
     7741 caccaccgaa ccggtgcgcg agttaatcac tactttagcg tcctgcgggg tgacattaac
     7801 ctgcatattc tggatatcgg caaggaagcg gacctgggaa ctgttgccac tcggtacgcg
     7861 cacctgaata gtccgcgcat ctaacgcggt ggcgctgcca tatccacgca cgcggttgat
     7921 ggtgtcagcg atttgctgcg ccatgctgaa atcttcgtcg ttaagttgca aattaagggt
     7981 attcccgacg ccaaactggc tgggcaattc acgttcaata accgcaccat tggtgatccg
     8041 tccaccgttc agttggttaa cctgaacact gctaccgcca gcggaggctc ctgcgccgct
     8101 aaccagaata ttgccctgcg ccagcgcata cacctgactg tcaacgccct taagcggtgt
     8161 catcaacaac gtacctccac gcaagctttt ggcattaccc atggaagaaa ccaccacatc
     8221 gatggtttgc ccctgacgtc caaacggagg aagtgacgct gtcaccatta ccgcagcgac
     8281 gttttttagc tgcatattgg tgcccgtcgg aacggtaatt cccagctgtg agagcatgtt
     8341 attaagcgtt tgtgtggtaa acggcgtctg ggttgtctgg tcaccggtgc catccagccc
     8401 caccaccaga ccatagccaa tcagtgagtt ttgccttacc ccctgaacac tggtgagatc
     8461 gcgaatacgc tcagcctgag ccgccgtcgt gaccagtaga agaattaatg cagagagaaa
     8521 tttaatcacc acagcctcac ttacattggc gacaggttaa ggaagaaacg ctgcaaccag
     8581 cccatatttt gcgcttcgtt aatgtagcca ttgcctacgt attcaatgcg cgcatccacc
     8641 acctgagtag acggtacggt attgctgccg ctgatagtgc gtggattaac cacgccagag
     8701 aagcgaataa attcggtacc ctgattaatg gcaatctgtt tttcacccac cacatgcagg
     8761 ttgccgttga ccagtacctg gtcaaccgtc accgtcaacg tgccgctaaa ggtattgctg
     8821 gcattggccc cgccctttcc gttgaacgtg ttaccaccgg aggcttcgac atcggcacga
     8881 gcgttaccaa acagcccctg caaatagcgc ggcacagtat caaagccaaa attagtttta
     8941 ccgtcacggc tggcattcgc agaggagctt ttgctggcgc tgacgttctc ctgcaacacg
     9001 atggtcagcg tatcgccaat attgcgtggt cgacgatctt caaacagcgg ttgatagcca
     9061 tagttaatcg gctgagcaga ctggaaaata gaaccgttgg cgacgggcgt cggaccggga
     9121 accggttgtg cactggtcgc cccctgcacc agcggcgtgg agggtatcca ggcgcagccg
     9181 gttagtgaaa gcaccaacaa gctggaaatg gcataagtat gcgcagcgtt tttttgcatg
     9241 gctatcatct tcaaaaatca gtaaaccggt gaacctgcca ccggttaagc cttagagttg
     9301 cgtcagtttt tgctgcatct gatcggtggt ggacaccgct ttactgttga tttcgtaagc
     9361 gcgttgcacc tgaatcatat tgaccagttc ttccgccacg ttgacgttag acgtttcaac
     9421 atacccttga tacagcaatc ccgcgccgtt caggcccggc gtgctttcgt tcggtgcacc
     9481 agaggattgc gtttcggtgt agaggttttc gccaatgctc tccagcccgg tgtcattcat
     9541 aaaggtggtg agattgagct gcccaacctg aaccggagct gcctggcctt gttgggttac
     9601 gctgaccacg ccatcacgac cgatggtgat acttaacgca ttcgccggaa tggtgatcgc
     9661 tggctgcacc tgaaaaccac cagccgtcac cagctgcccg ttctgatcca cctggaaaga
     9721 gccgtcacgg gtataggctg atgaaccatc tggcaacatc acctggaaaa agccctgccc
     9781 tttaatcgcg acatctttgc tgttgttggt ctgcgacaga tttccctggc tgtgtaagcg
     9841 ttcagtggcg accgggcgta cccccgtgcc gatttgtaat ccggagggta aggtggtttg
     9901 ttcggaagac tgtgcccccg gctggcgaat ggtttgataa agcagatctt caaacaccgc
     9961 gcgctgacgc ttaaaaccgt tagtactgac gtttgccagg ttgttggcaa tgacgtccat
    10021 attggtttgc tgggcgtcaa ggcccgtttt ggcgatccat aatgaactga tcataaaatg
    10081 tcctgtagtg gattagtgac aagcagagca gaagcacgcg acagtgtgaa ttccccctca
    10141 ctctaaccct ctccccaaag gggcgaggga atagtccgcg cgcttttatt gcaatgtcat
    10201 caatcgttca attaactcat cgacagcagt tggttggcac ggcctgcgtt atcatcgacg
    10261 ctgctgatca ccttcatctg catttcaaaa cgccgcgcgc tggcaatcat gtcgctcatt
    10321 gccgcaacgg cattgacgtt actgccttcc agaacccccg acatcacacg caaggttgga
    10381 tctgcctgca gtaccggccc tcgcgtggcc tgggtttctg cgcttaaacg aaaaatgccg
    10441 tcgtcaccgc gctgcacttc gctgcccgtg gctttcacca gtttcagacg ccctactggc
    10501 gcaactgtat ttgccggatc gcccggattg agcgccgaga ttgtgccatc ggcagcaata
    10561 gtgatttccg ctccttccgg cacagcaatt ggccccgcct cgcctatcac ctgatgcccc
    10621 tgaattgtca gttgcccggt gggatcaacc tgaatgctgc cattacgcgt atacccttcg
    10681 ctgccgtcag cggtctgcac ggccagccag ccatcctgct gcaacgcaac gtccagcggg
    10741 cgcgaggtgt aatctatctt gccgggcgtc atgtctgcac ccggtgttga cgccgttacc
    10801 agcgtgcgcg tgggcagtga aagcccttcc accggcactg cgcgcaaggc gtttaactgc
    10861 gcgcgaaaac cgggcgtaga ggcattggcc agattactgg cggttaccgc ctgttgattc
    10921 agcgtctggc tggctgctcc catcgcagta taaattgcgt gatccattga gctatcccgt
    10981 cagcgattag cgtaagttaa ccagcgtgtt gaggatctgg tcctgggttt tgatggtctg
    11041 ggcgttagac tgatagttac gctgggcaac gatcatattg accagttctt tactgagatc
    11101 gacgttggac gcttccagcg caccgttggt cagggtgcca aagtttcccg tcccggctgt
    11161 ccccaacagc gccacgccag aagattgcgt cgcagaccag acgttgtcgc cttcggatgc
    11221 cagaccttcg ttgttggcaa agttcgccag tacaatctgc cccagcagtt gagtttgttc
    11281 gttggaatag ttgccgacaa ccgtaccgtc atcattgatt tgataactca ccagatcgcc
    11341 cggtttgtag ccgttctggg gggttgccac aatattgtta gcgccggtat tttgctgcat
    11401 ggagttgagg aagctcagac taaacgtggc gggttctgca ccgttaattg cgccggttgc
    11461 gatattattc gccatcgcac catccactaa tgtgccatta gcattaaatt ccagtgttgt
    11521 cgctgtcttc gcaatgctgt ttggatcact gctatcctgg gtgtagacct gccagttatt
    11581 atccccggtc ttcacaaagt agacgctcat gtcatgagca ttaccctgac tgtcgaaaac
    11641 agtcaccgaa ccttttttgt tatagctatc cgcattgctg gcgctgaatg gcgtaacagt
    11701 aggaagcgga tcactggaat tcaggttgat ctgcatcgac gccgtggtgg tagttttcgc
    11761 tgccatcagg gtattcggga tcgaaatatt ggtcggattc gccccttgct gaatagtcgg
    11821 cggcgtaccg gttgccgggt aacccgtcag ctgtaaacct tgcatattca ccaggttacg
    11881 gttttcatcc agcttaaatt gtccgttacg gctgtagaac accgaaccgt tgctgtctac
    11941 cagacggaaa aaaccgttct ggctgatagc aacgtccaga cctcgcccgg tgttggtggt
    12001 cgtgccatct gtaaagtcct gagtgatacc ggcaactttt acccccagtc ccactttcga
    12061 accggcaaac atatcggcaa aagaggccgt gcctgattta aagccgtagg tggcggagtt
    12121 ggcaatattg ttgccaataa catcgaggtt agtggcggca gcgtttaatc cgctaaccgc
    12181 ttgagaaaag gccatgactg actcctgaaa tgtgaaggct taaattatct gccgtacttc
    12241 gtcgagggtg gtggtgccgt aagtgccgag atccagcgta ttaccgctgt tgccgcggat
    12301 cacaccctgc accagagcaa actgcagcgg ctgggcaacc agttgtgtac caccgttact
    12361 ggcggtaatc gctacattgt aagaaccgtt cggcgcagtg ctgccatcag tcaacgtacc
    12421 gtcccaggtg aaactgtgaa ctccggcggt cagttcacca atatcaatgg tgcgcacaac
    12481 cgcgccattt ttatcggtga tggtggccgt aactttgtct gccgcctgtt gcagctcaac
    12541 accaaacggc gtggtcgtgg tcacagcccc ttcttcactg ccggttcccg caagaacagt
    12601 tgtgccgggg atcatcacgc cgtgaccgat caggttactg gcctgtaacg actggctgtt
    12661 atcaatctgt ccggaaatag atccgagcat ggtattgagt ttttcaatcc cgctgaccgt
    12721 gctgatttgt gccaattgcg acgtcagctc gttgttttcc attggattgg tcgggtcctg
    12781 gtttttcagc tgcgccacca gcaaagtcag aaaactgctt tgtaaatctg cggcgttgct
    12841 gcccgtgagc gaactactgc tggtggtact gacgccggta tttgtcggat cggtggtggt
    12901 taccgcaatg gacatagctt tctcctttat tgaccgagcg taagggtttt cagcatcatg
    12961 cttttcaccg tgttgagcac ttcaacattg gcctgatagc tgcgtgacgc cgacatggtg
    13021 ttaaccatct ctccgacaac atcaacgttc ggcattttta cgtagccctt tgcatccgcc
    13081 agcggattac ccggttcata aaccagtttg tccggggcct gactttctat aacatcggca
    13141 acctttacgc cgcctgtcgc agcacctggt gcagcgttaa cctggaatac cacctgtttt
    13201 gcccgatatg gctgtccatc gggaccggtc acgctatcag cattcgccag attactggcc
    13261 gccacgttca ggcgctggga ctgggcagtt aacgccgacc cggcgatatc aaaaatattc
    13321 agcagtgcca tccgttaatt tccgctctgt aaaacgttca tcatgccttt gatttgcccg
    13381 ctcaacgcac taaggctcat ctggtattgc agttctgcgg taggaggcgt cagcgcctgc
    13441 gccggaatgt gttgcgttga ggtcatcgtc agagcaacca cactggttgc atcccgtcca
    13501 cgttgcatga cttttttaag ttcactggca aaatcgatat cgcgcgcctg ataaccaagg
    13561 gtatcggcat tggcgatgtt tgctgccagc acttcctgac gctgggcgcg cagattgagc
    13621 gcctcttgtt gaaaacgtaa ggcggcgtcg agcttatcga gcatatctcc tccgcaggta
    13681 tcaaaattct gccatcagct taaatgcctt tacgcgcgcg ttatcggcgg aataaacgca
    13741 aaatgggtcg ctatttatgc cgttgatgac cattggggac gggtacaatt cacgttgtag
    13801 aaatagctgg ggggcgaaaa tgctggcaat aaaacgtagc gtggcgatca tcgcgatact
    13861 gttcagtccg ttaagtgcgg cgagcaatct cacatcgcaa ttgcacacct tttttagcgc
    13921 ccagctcgtg ggggtaagtg atgaggttcg tgtttctatt cgtacagcac ccaatctact
    13981 accgccgtgc gagcagccat tgctttcgat gagcaataat tcccgcctgt ggggcaatgt
    14041 gaatgtgttg gcacgctgcg gtaacgacaa acgatattta caggttaatg tgcaggccac
    14101 aggaaattat gtggttgccg cgatgcccat tgtgcgggga ggaaagctgg aagctggcaa
    14161 tgtcaaacta aaacgcggac gactggatac cctgccaccg cgtacggtgc tggatatcaa
    14221 tcaacttgtt gatgccatta gcctacgcga tctatcaccc gatcaaccaa tccaattaac
    14281 ccattttcgc caggcatggc gggtaaaagc gggacaacgc gtcaacgtga tcgccagcgg
    14341 tgacgggttt agcgccaacg cagaaggtca ggcgctgaac aatgcagccg tcgcacagaa
    14401 tgcgcggatg cgcatggtat cgggactggt ggtcagcggc gttgttgatg cagatgggaa
    14461 tattcttata aacctgtaag ctgttaaaga ttacccgtcc cttgccgata aataagcaac
    14521 acatgataaa agcgccctca atgaggaata aaccatgagt attgatcgca cttcgcctct
    14581 gaatcccgta agcaccgttc aaccgcgcga aaccactgac gcgccggtaa cgaacacccg
    14641 ggcggcaaaa acaaccgact ccaccagcac cagcaccagc accagcacca gcaccagcac
    14701 cagtgtgacg ttaagcgacg cgcaagcaaa actgatgcaa cccggcagca gtgatatcaa
    14761 tcttgaacgt gtcgaagcgt taaaactggc gattcgtaac ggtgaactaa aaatggacat
    14821 cggcaaaatt gccgatgcgc tgatcaacga agctcagcaa gacttgcaga gtaactgatc
    14881 gtatgacacg tcttgcagag atccttgacc agatgtccgc tgtgcttaac gatctcaaaa
    14941 cggtaatgga tcaagagcag caacatctct ctatggggca gatcaacggc agccagttgc
    15001 aatggattac agaacaaaaa agctcactgc tggcgacgct ggattacctc gaacagttac
    15061 gcaagaaaga acccaataca gcaaatagcg ttgatattag tcaacgctgg caggaaatta
    15121 ctgtgaaaac gcagcaacta cgccaaatga atcaacataa cggttggtta ctggaaggac
    15181 agattgagcg caatcaacag gcgctggaaa tgttgaaacc gcatcaggaa ccgacgctat
    15241 atggggcgaa cggtcagacc tcaacaaccc atcgcggcgg taaaaagatt tcgatctgaa
    15301 gattatctcc ggcctgcact gcaggccgga atgcattgtt acaccgtccg gcgggcaaat
    15361 tctttaactt tgaagcccag taccgccagt gcagcgaagt acgcggcaat ccccgccagc
    15421 acgaccgcca ttaaacgcag taagcgccag ggcatggtac ccaatgacca ctccggcatg
    15481 atatgtaaca tacctaaaag cacgccagac atcaccagta ccgccaccac cagacgcaac
    15541 agaaacgcca tccagccggg ttgcggagta aagattttct gcttacgcaa ctgccagtaa
    15601 agcagcgaag cattcagaca cgccgccaga ccaatagaaa gtgacagccc ggcatgtttc
    15661 aacggaccaa taaacgccag gttcatcaat tgcgttaaaa tcagcgtaac gatggcaatt
    15721 ttcactggcg ttttaatgtc ctggcgggaa taaaagccag gagccaacac tttcactaca
    15781 atcaggccaa tcaaacccac cgagtaggca attaacgccc gctgggtcat cagcgcatca
    15841 aacgcggtaa atttaccgta ctggaacagc gaaacggtta acggacctga aagaatgccc
    15901 aacgcaaccg cactcggcag cgccaacagg aaacaaagac gcaaccccca gtccatcaaa
    15961 cggttgtatt catcatgatt gccactggca aaacttttcg acagcgacgg cagcaaaatg
    16021 gtgccaagcg ccacgcccag cacaccggac ggaaactcca ttaagcggtc ggcgtaatac
    16081 atccaagaca ccgaaccgga agcaagaaac gaggcaaaaa tggtgttgat gattaaggag
    16141 atctggctca cagagacgcc aaggatcgcc ggtcccatct gtttcaccac gcgcattgct
    16201 ccggcatcgt ggaagttaat gcgcggcagg accagcatgc cgatcttctt caggtgcggt
    16261 agctgataca ccagctgcag gatgccgccg accgtaacag cccacgccag cgccagcacc
    16321 ggtgggttaa agtacggtgc ggcaaacagc gcgaaaccaa tcatgctgat gttaagcagt
    16381 gttggcgcaa acgccggaat cgagaagcgg ttccacgtat tcagaatcgc tcccaccagc
    16441 gacgccaggg agatcagcaa gatataggga aaggtaatct ttagtagctg gctggtcagg
    16501 gcaaatttgt cagctgtatc agcgaagcct ggcgcggtca ccatgatcac ccacggtgcg
    16561 gcgagcatgc cagcgaccgt cacaaccgcc agcgcaagtg tcagcaggcc agaaacataa
    16621 gagacaaaga cccgcgtggc gtcttcaccc tgcttacttt tatattccgc cagaatcggt
    16681 acaaatgcct gggaaaatgc cccttcggca aagatacggc gtaacaagtt aggaagttta
    16741 aaagcgacga aaaaggcgtc ggttgccatc cctgcgccaa agattctggc gacaattgcg
    16801 tctcgtgcga agccaagcac acgcgaaaac atggtcatcg agctgacggc ggccagcgat
    16861 tttaataaat tcatcggtgt tctaatccag accaacgggc aaaacgcctg caagacaggc
    16921 gctgaaagag gcgaatagtc tacctggatt atggtgaatt gctaccgcca gatgttacag
    16981 ggtttattca ctcatcgcat cgcgccagat cttgtcaacg atacgttgcg ccagcacggc
    17041 ctattcgccg gcggtttgcg gaactgtctg attttgcaca cattcaatga agtgccgcgc
    17101 acagccgaca aatccacgtt gttcaagagt gctctgccaa ccaggaatcg gtttatgcac
    17161 tacgccctgc ccgcgctcct cacgccattc gcgcatatcc gtaatgtcga tgagcgcacc
    17221 gtcagtcacg gcctgcacgg tttcacgctg acttccggca cggcgatgca tacaggtggt
    17281 gatctgcaaa ggaccggccg aaaaatggtg ctcggcaaac agcatttcgc cagcgtcgtt
    17341 agtcagtagc gtaccgccat ccagagaggc tttgccgccc gacaaccaca gcgcggtatc
    17401 caccacatgc agataatcat ccagcaacgt gaaataaaga tcgtgtggcc cgacactatt
    17461 gctacgatgt ttatccattc ttagcgaagc tgcggtagcg agttgcgttt ttaactcacc
    17521 gtagagtggt gcgaaacgac ggttaaaacc gaccatcaac gtcagttttt tccgcgccgc
    17581 cagttccacc agccgttcag catcgcgcag attttctgcc agcggtttat cgacacagac
    17641 atgtacaccc gcattgagta acgtactgac cacgtcaaag tggctggcgg tgctggaatg
    17701 cacaaaaacc gcatcgcaac tggcggcaag gctggataac gaatcggcat aaggaatgcg
    17761 ccagctttca caaattggca gggctttcgc gcgcgtaggc gaccaggctc cttgtaacat
    17821 ccagtcagac gctgccgcca gcaccggtaa ccacgctttt tgcgcaatgc cgcctaatcc
    17881 cacaacgccg atacgtaatt ttttcacttt aatctcccag atgggccagc aacgaatcaa
    17941 gacgctgttt cagttctgcc acttcgattt ccagggcttc gacgcgggcc tgtaaatcac
    18001 catcaaccgc gttcgacata tccgttaccg ccggctgatc ctctacctca ccactgaaaa
    18061 gatgcatgta gcggctttcg cgtttacccg gttcgcgggc cagacgcacc acaaaaggac
    18121 catcttcgcg atttgccagt tgttccagcg tcgactccaa ttccgccata tcgctgaatt
    18181 catacattcg cgcggcgcgg ctgcgcagtt cacccggcgt ctgggcacca cgcaataaca
    18241 acgtggtgat taacgccact tccgctgcgc tcagtttcag atcgccaaat tctgaattac
    18301 aaaaacgttg ttcatatttg gtgacccgat taccaaagcc actcaccgtg cgcagataat
    18361 ggcgtttgac cagattatcc agttgctcct gcacttcaga ttcactcaga ttcatgaccg
    18421 gttcacggtt cgttttctga ttacaggccg tgactacacc attgactgag agcgggtatt
    18481 gctccggcgt ggtcacctgc ttttccagca agcaaccaat cacgcgagct tcaagggcag
    18541 taagttgata tttcatgaga ttctccttag cggccgggcg tccagtctgg ggtagttaat
    18601 gccgtcagta cgtgatcgcg ccattgtcca tcaatcaaca gatagtcttt cgcatagcct
    18661 tctttttcaa aacccagtcg cgccagtaaa tcaccgctgc gtttattgtg cggcatataa
    18721 ttagccataa tgcgatgaat atgttgggtg cgctgcatat aacgaatggc tgcggtcagg
    18781 gcttcaaaca tgagtccttt gccctgccat ttttgcccaa tcgaataacc gagatagcag
    18841 gcatgaaaag agccacgaac aacattggaa aaattggcaa cgccaataat ctctttttcg
    18901 tccgggtcga ataagccaaa gtagaaagct gaaccttgtt tatgaaattc gttaatcatc
    18961 cccagcctgg cctgccagcc tgatggataa cagtggcttt cgtcgcgcac tggctcccag
    19021 ggcttgagga aatggcgatt ctctgcgtaa taatccgcaa gacgccaggc atcacgatca
    19081 tgcaccagac gcacgaccag tcggtctgtg gttaagcgca cttttggcac gttactgcga
    19141 tagccaaaca ttctatacct actccttccc gtaacgtctt taatacgctg taagtattac
    19201 tatacctgcg caaccagacc ctgtgaaaag tccaaaatgc cattttctgc attattcaca
    19261 tatttgataa aaactctcta tcaaagcgac gttttgataa aaaaatattg tccccgaagt
    19321 gatgggaaaa gtcttagcgg caatgcagaa tagaaagtgc tcatctttta tttcccggga
    19381 ggggaaatgt cccgcgtatc gcaggcgagg aacctgggta aatatttcct gctcatcgat
    19441 aatatgctgg tcgtgctggg gttctttgtt gtcttcccgc tgatctctat ccgcttcgtt
    19501 gatcaaatgg gctgggccgc cgtcatggtc ggtattgctc tcggtctacg ccagtttatt
    19561 cagcaaggtc tgggtatttt cggcggcgca attgccgacc gctttggtgc caaaccgatg
    19621 attgttaccg gtatgctgat gcgcgccgcc ggattcgcca caatgggtat cgcccacgaa
    19681 ccgtggctat tgtggttttc atgcctgctc tcgggactcg gcggcacgtt gtttgatccg
    19741 ccgcgttcgg cgctggtggt gaaactaatc cgtccacaac aacgtggtcg ttttttctcg
    19801 ctgttgatga tgcaggacag tgccggtgcg gtcattggcg cattgttggg gagctggctg
    19861 ttgcaatacg actttcgcct ggtctgcgcc acaggggccg ttttgtttgt gctgtgtgcg
    19921 gcgttcaatg cgtggttgtt accggcatgg aaactctcca ccgtacgcac gcccgttcgc
    19981 gaaggcatga cccgcgtgat gcgtgacaag cgttttgtca cctatgtcct gacgctggcg
    20041 ggttactaca tgctggctgt acaagtgatg ctgatgctgc caattatggt caacgacgtg
    20101 gctggcgcgc cctctgccgt taaatggatg tatgccattg aagcgtgtct gtcgttaacg
    20161 ttgctctacc ctatcgcccg ctggagtgaa aagcattttc gtctggaaca ccggttgatg
    20221 gctgggctgt tgataatgtc attaagcatg atgccggtgg gcatggtcag cggcctgcaa
    20281 caacttttca ccctgatttg tctgttttat atcgggtcga tcattgccga gcctgcgcgt
    20341 gaaaccttaa gtgcttcgct ggcggacgca agagctcgcg gcagctatat ggggtttagc
    20401 cgtctgggtc tggcgattgg cggcgctatt ggttatatcg gtggtggctg gctgtttgac
    20461 ctgggcaaat cggcgcacca gccagagctt ccgtggatga tgctgggaat tattggcatc
    20521 ttcactttcc ttgcgctggg ttggcagttt agtcagaaac gcgccgcgcg tcgtttgctg
    20581 gaacgcgacg cctgatttga tactcccccc tgactcctcc atactgaaag tgaactgaca
    20641 gccaaatgga ggagtcacgt gaagctatac atttacgatc actgccctta ctgcctcaaa
    20701 gcccgcatga ttttcggcct gaaaaatatc cccgtcgaat tacatgttct gctcaacgac
    20761 gacgcagaaa cacccacccg gatggtcggt caaaaacagg ttcccattct gcaaaaagat
    20821 gacagccgct atatgccaga aagcatggat atcgttcact atgtcgataa actcgacggc
    20881 aaaccgttac tgaccggcaa acgttcccct gccattgaag agtggctgcg caaggtcaat
    20941 ggctacgcca acaaactgct gttgccgcgt tttgccaaat cggcatttga tgagttttct
    21001 actcccgccg cgcgcaaata tttcgtcgac aagaaagagg ccagcgcggg taattttgcc
    21061 gacctgctgg cccactctga cggtctgatt aagaatatca gcgatgattt gcgtgcgctg
    21121 gacaaactga tcgtcaaacc gaacgccgtg aatggagaac tttcggaaga tgatattcag
    21181 ctatttccgc tactgcgtaa tctgacgctg gtagccggaa tcaactggcc aagccgcgtt
    21241 gctgattacc gcgataatat ggcgaaacag acacaaatca atttgttatc atcaatggcg
    21301 atttaatcct gacccgccgc ggcagcctgt attcgcgggt tgccgccgtt ttcagttttt
    21361 tctgctggcg agtttatcgc tggtgacaca tgctgcgttg tgtcaggata ttgacgcaga
    21421 tttcatgtcg aggactacca tgaacaagtt tttatttgcc gctgcattga tcgtcagtgg
    21481 cctgctcgtt ggctgtaatc aactcaccca atacaccatc accgaacaag aaattaacca
    21541 gtcgcttgcg aaacataata atttctcaaa agatatcggt ttacccggcg tggctgacgc
    21601 ccatattgtt ctgacaaact tgaccagcca aattggtcgc gaagagccga ataaggttac
    21661 cctaaccgga gacgccaatc tggacatgaa ctccctgttc ggtagtcaga aagcgaccat
    21721 gaaactgaag ctgaaagcgc tgcccgtgtt tgataaagaa aaaggtgcga tcttcctgaa
    21781 agagatggaa gtggtcgatg cgacggtaca accggaaaaa atgcaaacgg tgatgcaaac
    21841 gttgcttccc tatttgaacc aggcattacg caattacttt aaccagcaac ctgcttacgt
    21901 cctgcgcgaa gatggcagtc agggcgaagc aatggcgaaa aaactggcga aaggcattga
    21961 agtgaagcca ggcgaaattg tcattccgtt tacggattaa tgacgagggc gcattcgcgc
    22021 cctttatttt tcgtgcaaag gaaaacgttt ccgcttatcc tttgtgtccg gcaaaaacat
    22081 cccttcagcc ggagcataga gattaatgac tgcaccatcc caggtattaa agatccgccg
    22141 cccagacgac tggcaccttc acctccgcga tggcgacatg ttaaaaactg tcgtgccgta
    22201 taccagcgaa atttatggac gggctattgt aatgcccaat ctggctccgc ccgtgaccac
    22261 tgttgaggct gccgtggcgt atcgccagcg tattcttgat gccgtacctg ccgggcacaa
    22321 tttcacccca ttgatgacct gttatttaac agattcgctg gatcctaatg agctggagcg
    22381 cggatttaac gaaggcgtgt tcaccgctgc aaaactttat ccggcaaacg caaccactaa
    22441 ctccagccac ggcgtgacgt caattgacgc aatcatgccg gtacttgagc gcatggaaaa
    22501 aatcggtatg ccgctactgg tgcatggtga agtgacacat gcagatatcg acatttttga
    22561 tcgtgaagcg cactttatag aaagcgtgat ggaacctctg cgccagcgcc tgactgcgct
    22621 gaaagtcgtt tttgagcaca tcaccaccaa agatgctgcc gactatgtcc gtgacggaaa
    22681 tgaacggctg gctgccacca tcactccgca gcatctgatg tttaaccgca accatatgct
    22741 ggttggtggc gtgcgtccgc acctgtattg tttacccatc ctcaaacgca atattcacca
    22801 acaggcattg cgtgaactgg tcgccagcgg ttttaatcga gtattcctcg gtacggattc
    22861 tgcgccacat gcacgtcatc gcaaagagag cagttgcggc tgcgcgggct gcttcaacgc
    22921 cccaaccgcg ctgggcagtt acgctaccgt ctttgaagag atgaatgctc tgcagcactt
    22981 tgaagcattc tgttctgtaa acggcccgca gttctatggc ttgccggtca acgacacatt
    23041 catcgaactg gtacgtgaag agcaacaggt tgctgaaagc atcgcactga ctgatgacac
    23101 cctggtgcca ttcctcgccg gggaaacggt acgctggtcc gttaaacaat aaaaatcgta
    23161 gcttcctgtt gtcattaggt tattttacct gtataaataa ccagtatatt caacaggggg
    23221 ctattatgcg aattgaagtc accatagcga aaacttctcc attgccagct ggggctattg
    23281 acgccctggc tggcgaactt tcccgccgta ttcagtatgc gtttcctgat aatgaaggcc
    23341 acgtatcggt acgttatgcc gcagcgaata atttatcggt tattggcgta acaaaagaag
    23401 ataaacagcg cattagcgaa attctccagg aaacgtggga aagcgccgat gactggtttg
    23461 tcagcgaata atatgcagtg atttttgttt ttgccgggtc gccccggctt ttttatttta
    23521 cttacaaatt ttgagtacaa aatttctaca cttcctcaaa ataattgcac aatatgctaa
    23581 aaaaatatga atgacatgct gttttattgt tcatataaat cttaaaagca gaaatttatc
    23641 tgttcccccc tttaaaaatt cggtgaatac ccttacttat tggtatactg aagttgcttc
    23701 agataaaaac gcattgaacc tcgaataacg ttgtctagta acacgaatta gggggccatg
    23761 atggaaaaaa ataatgaagt cattcagact catccgctcg tagggtggga catcagcacc
    23821 gttgatagct atgatgcgct gatgttgcgt ttgcactacc agaccccaaa taagtccgag
    23881 caggaaggga ctgaagttgg tcagacgctc tggttaacca ctgatgttgc cagacaattt
    23941 atttcgatat tagaagcagg aatcgccaaa attgaatccg gtgatttccc ggtaaacgag
    24001 tatcggcgtc attaacatga catgaccatc cacccaaaag gcacctcacc ggtgccttta
    24061 ccatttctgg gttgtatatc tgcattcagt taattactct gcttaaacca tgagagcaga
    24121 gagaaaaaaa tgaaatacga tctcatcatt attggcagcg gttccgtagg cgctgccgcc
    24181 gggtattatg caacccgcgc cggtttaaac gtgctaatga ccgacgccca tatgccaccg
    24241 catcaacacg gcagccacca cggcgatacg cgattaattc gccatgctta tggtgaaggc
    24301 gaaaagtatg tcccgttggt cctccgcgcg caaacgctgt gggatgaact ttcccgccac
    24361 aacgaagatg atcccatttt tgtacgctct ggtgtcatta acctcggccc ggctgactcc
    24421 gcatttctcg ccaacgtcgc ccacagcgcc gaacagtggc aactcaacgt tgaaaagctc
    24481 gatgcgcaag ggattatggc ccgctggcca gaaatacgcg tcccggacaa ctacatcggc
    24541 ttatttgaga ctgattccgg ttttttgcgc agcgaactgg cgattaaaac ctggatccaa
    24601 ctggcgaagg aagcgggctg tgcgcaactg ttcaactgcc cggtcaccgc aattcgtcat
    24661 gacgatgatg gcgtaactat tgaaaccgtt gacggtgagt atcaggcgaa aaaagcgatt
    24721 gtctgcacgg gaacatgggt aaaagacctg ctcccggagc tgcctgtcca gcctgtacgt
    24781 aaagtatttg cctggtatca ggccgatggc cgctatagcg tgaagaataa attcccggcg
    24841 tttaccggtg aactgcccaa tggcgatcaa tattatggtt ttccggcaga aaacgacgcg
    24901 ttgaagattg gcaaacataa cggaggccag gttatccatt cagcggatga acgtgttccg
    24961 tttgcggaag tggtcagcga tggttcggaa gccttcccgt tcttgcgcaa tgtattgccg
    25021 ggtatcggtt gctgcctgta cggcgctgcc tgcacctatg ataattcgcc tgacgaagat
    25081 tttattatcg ataccctacc cggccacgat aatacactgc tcattaccgg cctgagtggg
    25141 cacggtttta aatttgcgtc agttttaggg gaaatagctg ccgattttgc gcaagacaaa
    25201 aaaagcgatt ttgatttgac gccattcagg ctttcccgct tccaataatc atcattacgg
    25261 cctccagaat gaggccgttt ttacaggagt atttaatgcg tcggctgttg cactatctca
    25321 tcaataatat tcgcgagcat ctgatgcttt atctttttct ctggggattg ttggcaatta
    25381 tggatctgat ctatgtgttt tatttttagt tttccgcact gacataaact gacaaatatt
    25441 taatctccct ttcatttagc gtgaaaaatt gaatgaaatt attcctgctt ctacagcact
    25501 caccagacat ctctgatgct gaacccacta aacttatgaa gattctttca ttattaattc
    25561 aaaattattg aaataaaaat aaatatcatt caatttgtta tttttgtgtt gcaattcatt
    25621 ctccgacacg ccattttctt tctctcgcaa attcgtattg gatgcactta tgtcattcac
    25681 aaatacccct gaacgctatg gtgttatatc tgcggccttc cattggctaa gcgcaattat
    25741 tgtctacggc atgtttactt taggtctgtg gatggtcacg ctcagttatt acgatggctg
    25801 gtatcacaaa gcacccgaac tgcataaaag catcggtatt ttgttaatga tggggctggt
    25861 tattcgcgtt ctgtggcgtg tcatatctcc accgccgggt ccgctgccga gttattcgcc
    25921 catgactcgt cttgccgcaa aggctggaca tctcgcattg tacctattgc tgttcgccat
    25981 cggcatcagc ggctatctga tctcaactgc cgatggtaaa ccgatcagcg tttttggctg
    26041 gtttgacgtc cccgcgaccc ttgccgacgc tggcgcacag gcagactttg ccggtgccct
    26101 gcatttttgg ctcgcgtgga gcgtcgtagt actgtccgtt atgcacggat ttatggccct
    26161 gaagcatcat ttcatcgata aagacgacac tctgaagcgc atgctgggaa agtcgtcatc
    26221 tgactatgga gtataaaaaa tgaaaaaaag cctgcttggt ttaaccttcg cgtccctgat
    26281 gttctctgcc ggttcagcgg ttgccgccga ttacaaaatt gacaaagaag gtcagcacgc
    26341 ctttgttaat ttccgcatcc agcaccttgg ctatagctgg ttatacggca cctttaaaga
    26401 tttcgacggt acttttacct ttgacgaaaa aaatccggct gccgataaag tgaatgtgac
    26461 aattaacacc accagcgtcg atactaatca cgccgaacgc gataaacatc ttcgcagtgc
    26521 agatttcctc aataccgcaa aatatccaca ggcaacattc acctccacca gcgtgaagaa
    26581 agacggtgac gaactggata ttaccggcga tctgacgctg aatggcgtaa ccaaacccgt
    26641 cacgctggaa gcgaaattaa ttggtcaggg caacgaccca tggggtggta aacgtgcagg
    26701 cttcgaggcc gaaggcaaaa ttaagctcaa ggacttcaat atcaagacag atttaggtcc
    26761 agcttctcag gaagtggatc tgattatttc agtggaaggc gtacagcaga agtaagcgtg
    26821 aaatgttgcc ggatggcacg catccggcaa tgatatttat tctgttggat caggaatgcc
    26881 cagtgttgta ttcagacgtc cacgagactt attaaagatc ttattgccat tttcacgtcc
    26941 tgcccgacgg cgtcgctgtt cttctggcgg taacgcgctt tcttcgcagc aaatctcact
    27001 acaacaacct ttgtattttt ccgcgcatac tggacactga ataaacagca ggtggcagcc
    27061 atcattttta cagttggtat ggctgtcgca cggcgcgccg cactggtggc aatgcgcgat
    27121 aatctcatcc gatatacgtt cgcccatccg ctcgtcaaaa acaaaatttt tgccaataaa
    27181 acgcaccggc aagccctgct cgcgcgcctt acgggcgtat tcaataattc cgccctcgat
    27241 atgccagact ttgttgaatc cgttatgttt catccaagca ctggcctttt cacaacgaat
    27301 gccgccggtg cagtacatga cgattttttt atctttatgt gcctgcatca tctcgactgc
    27361 ttttggcagc tgctcacgga aggtatctgc cggaatttcg agcgcgtttt caaagtgccc
    27421 cacttcatac tcatagtggt tac
//
LOCUS       DAJTMC010000040        27203 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.040, whole genome shotgun sequence.
ACCESSION   DAJTMC010000040 DAJTMC010000000
VERSION     DAJTMC010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27203
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.040"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(37..1335)
                     /gene="eno"
                     /locus_tag="OO130_001507"
     CDS             complement(37..1335)
                     /gene="eno"
                     /locus_tag="OO130_001507"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HCR5364966.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(1423..3060)
                     /gene="pyrG"
                     /locus_tag="OO130_001508"
     CDS             complement(1423..3060)
                     /gene="pyrG"
                     /locus_tag="OO130_001508"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HCR5364967.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(3288..4079)
                     /gene="mazG"
                     /locus_tag="OO130_001509"
     CDS             complement(3288..4079)
                     /gene="mazG"
                     /locus_tag="OO130_001509"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HCR5364968.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEESYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(4150..4485)
                     /gene="mazF"
                     /locus_tag="OO130_001510"
     CDS             complement(4150..4485)
                     /gene="mazF"
                     /locus_tag="OO130_001510"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HCR5364969.1"
                     /translation="MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKRIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            complement(4485..4733)
                     /gene="mazE"
                     /locus_tag="OO130_001511"
     CDS             complement(4485..4733)
                     /gene="mazE"
                     /locus_tag="OO130_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HCR5364970.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW"
     gene            complement(4811..7045)
                     /locus_tag="OO130_001512"
     CDS             complement(4811..7045)
                     /locus_tag="OO130_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HCR5364971.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(7093..8394)
                     /gene="rlmD"
                     /locus_tag="OO130_001513"
     CDS             complement(7093..8394)
                     /gene="rlmD"
                     /locus_tag="OO130_001513"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HCR5364972.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVTVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHEVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     SALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            8451..11207
                     /gene="barA"
                     /locus_tag="OO130_001514"
     CDS             8451..11207
                     /gene="barA"
                     /locus_tag="OO130_001514"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HCR5364973.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(11439..12779)
                     /gene="gudD"
                     /locus_tag="OO130_001515"
     CDS             complement(11439..12779)
                     /gene="gudD"
                     /locus_tag="OO130_001515"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCR5364974.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIMIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGDRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLCARDNAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(12800..14140)
                     /locus_tag="OO130_001516"
     CDS             complement(12800..14140)
                     /locus_tag="OO130_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCR5364975.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELHVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKRFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPGGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(14142..15512)
                     /gene="gudP"
                     /locus_tag="OO130_001517"
     CDS             complement(14142..15512)
                     /gene="gudP"
                     /locus_tag="OO130_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HCR5364976.1"
                     /translation="MFRSATTSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRAT
                     LSIAGSEMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWS
                     MFTLLQGFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSI
                     FNSAQYFATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQ
                     KELEYIAAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYF
                     FITWFPVYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKT
                     PIVMGMLLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSG
                     GLFNMFGNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIEL
                     KPVAGQ"
     gene            complement(15929..16378)
                     /locus_tag="OO130_001518"
     CDS             complement(15929..16378)
                     /locus_tag="OO130_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HCR5364977.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(16396..17178)
                     /gene="truC"
                     /locus_tag="OO130_001519"
     CDS             complement(16396..17178)
                     /gene="truC"
                     /locus_tag="OO130_001519"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HCR5364978.1"
                     /translation="MLEILYQDEWLVSVNKPSGWLVHRSWLDRDEKVVVMQTVRNQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(17178..17507)
                     /locus_tag="OO130_001520"
     CDS             complement(17178..17507)
                     /locus_tag="OO130_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HCR5364979.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQHALILAELEKLDA
                     LFADDAS"
     gene            complement(18129..18674)
                     /gene="syd"
                     /locus_tag="OO130_001521"
     CDS             complement(18129..18674)
                     /gene="syd"
                     /locus_tag="OO130_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HCR5364980.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            18742..19590
                     /gene="queF"
                     /locus_tag="OO130_001522"
     CDS             18742..19590
                     /gene="queF"
                     /locus_tag="OO130_001522"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HCR5364981.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPKVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATCGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            19702..21066
                     /gene="ppnN"
                     /locus_tag="OO130_001523"
                     /gene_synonym="ygdH"
     CDS             19702..21066
                     /gene="ppnN"
                     /locus_tag="OO130_001523"
                     /gene_synonym="ygdH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HCR5364982.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKAVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
     gene            21623..22912
                     /gene="sdaC"
                     /locus_tag="OO130_001524"
     CDS             21623..22912
                     /gene="sdaC"
                     /locus_tag="OO130_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HCR5364983.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            22970..24337
                     /gene="sdaB"
                     /locus_tag="OO130_001525"
     CDS             22970..24337
                     /gene="sdaB"
                     /locus_tag="OO130_001525"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HCR5364984.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            24449..25204
                     /gene="xni"
                     /locus_tag="OO130_001526"
     CDS             24449..25204
                     /gene="xni"
                     /locus_tag="OO130_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HCR5364985.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHDEMPALRAAFEQRGVPCWSTSGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(25309..26446)
                     /gene="fucO"
                     /locus_tag="OO130_001527"
                     /pseudo
     CDS             complement(25309..26446)
                     /gene="fucO"
                     /locus_tag="OO130_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
     gene            complement(26474..27121)
                     /gene="fucA"
                     /locus_tag="OO130_001528"
     CDS             complement(26474..27121)
                     /gene="fucA"
                     /locus_tag="OO130_001528"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HCR5364986.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIAFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRPIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
BASE COUNT         6578 a         7296 c         6554 g         6775 t
ORIGIN      
        1 cggaggctgg catttttaaa tcagataaag tcagtcttat gcctggcctt tgatctcttt
       61 acgaccgttg tacggtgctt tttcgcccag agcttcttcg atacgaatca gctggttgta
      121 tttagcaaca cggtcagaac ggctcataga accagttttg atctggcctg cagcagtacc
      181 aacagccagg tcagcgatgg tagcgtcttc agtttcgcca gaacggtgag agataactgc
      241 agtgtagcca gcatctttcg ccatcttgat tgcagccaga gtttcggtca gagaaccgat
      301 ctggttgaat ttgatcagga tggagttagc gatacctttt tcgatacctt ctttcaggat
      361 cttggtgttg gttacgaaca ggtcgtcacc aaccagctgg attttgtcgc ccagaacttt
      421 ggtctggtat gcgaaaccgt cccagtcaga ttcgtccaga ccgtcttcga tagaaacgat
      481 cgggtactgt ttggtcagtt cttccaggaa gtgagtgaat tcttcagagg tgaacgcttt
      541 gttgccttcg ccagccagaa cgtatttacc atctttgtag aattcagaag ctgcgcagtc
      601 catcgccaga gtgatgtctt tgcccagttc ataaccagca gctttaacag cttcagcgat
      661 aacagccaga gcttcagcgt tggaacccag gttcggcgca tagccacctt cgtcaccaac
      721 agcagtgttc atgcctttcg ctttcagaac ttttgccagg tgatggaaaa cttcagaacc
      781 catgcggatg gcttctttca cagttttcgc gccaaccggc tgaatcatga attcctggat
      841 atcaacgttg ttgtcagcgt gctcaccacc gttgatgatg ttcatcatcg gaaccggcat
      901 agagtatttg cccggagtac cgttcagttc agcgatgtgc tcgtacagcg gcataccttt
      961 agcagctgca gcagctttgg cgttagccag agatacagcc aggattgcgt tcgcgccgaa
     1021 tttggatttg ttttcggtgc cgtccaggtc gatcatgatc ttgtcaatgc cagcctgatc
     1081 tttagcatct ttgccaatca gcgcctgagc gatcgggccg tttaccgcag caacagcttt
     1141 ggttacgcct ttacccagga aacgggattt gtcgccatcg cgcagttcca gagcttcacg
     1201 ggaaccagta gaagcacctg acggagcagc tgccataccg acgaaaccac cctccagatg
     1261 tacttcggct tcaacagtcg ggttaccacg ggagtcgatg atttcacgac cgatgatttt
     1321 tacgattttg gacattaggt tttcctcaag tcactagtta aactgaaact ccagacaaac
     1381 aacacgtacc cagggtacgc gttgccgttc taactttttt acttacttcg cctgacgttt
     1441 ctggaactcg ctggcggctt tcacaaagcc tgcaaacagc gggtgaccat cacgtggagt
     1501 agaagtaaac tccggatgga actggcaagc cacgaaccac gggtgattcg gaacttcgat
     1561 gatctcgacc aactgatcat ccccggaacg gcccgcaacg cgcagacctg catcttcaat
     1621 ctgtttcaac agcatgttgt tgacttcgta acggtgacga tgacgctcaa caattgtcgg
     1681 cgcattgtac agctggcgaa ccaggctatc gtcaaccaac tggcactgct gtgcgccgag
     1741 acgcatggta ccgccgagat cgctcttctc gctacgaact tcaacgttgc cgttttcatc
     1801 gcgccactcg gtaatcagcg ccacaaccgg gtacttacag tctggcacaa attccgtaga
     1861 gttggcgttc tccatgttgg caacatggcg agcgtaatca attaacgcca cctgcatacc
     1921 caggcaaatg cccagataag gaatattgtt ctcacgcgca aaacgcgcgg tcgtaatcat
     1981 gccttctacg ccacgatagc cgaaaccgcc aggtacgagg attgcgtcca gacctttaag
     2041 gatttcaacg ccgcgcgttt caacatcttg tgaatcgatc agtttgatgt tgacgctgac
     2101 acgattcttc agcccaccgt gtttcagtgc ttcgatcact gatttataag catccggcag
     2161 ttcaatgtac ttgccgacca taccgatggt gacttcactt accgggttcg cttcttcgaa
     2221 gataacctgt tcccattcgg acagattcgc ttccgggcag tttaagctga atcgtttaca
     2281 aatataatcg tccagcccct gagatttcaa caggcccgga attttataga tggaatcgac
     2341 gtctttcaga gaaataaccg ctttttccgg aacattacag aacaatgcaa tcttcgcacg
     2401 ttcgttcgcc ggaacagcgc gatctgaacg acaaatcagg atgtcaggct ggataccgat
     2461 ggagagcagc tcttttacag agtgctgagt cggtttggtt ttgacttcac cagacgctgc
     2521 catgtacggc accagcgtca ggtgcataaa cagagtgtgc tcacggccaa tttcaacagc
     2581 catctggcga atcgcttcga ggaacggcaa ggattcgata tcacctactg taccgccgat
     2641 ttctaccagt actacgtcat gaccttcgcc accttccagc acgcgctctt tgattgcgtt
     2701 agtgatgtgc ggaataacct gcacggttgc gccgaggtag tcaccgcggc gttctttacg
     2761 cagaacgtca gagtagatac gacccgtggt gaagttgttg cggcggctca ttttggtacg
     2821 aatgaaacgc tcgtagtgcc ccaggtccag gtcggtttca gcgccgtctt cagtaacgaa
     2881 cacttccccg tgttggattg ggctcatagt acctggatcg acgttgatgt acggatccag
     2941 tttcatgatg gtcacattga ggccacgggc ttcaagaatg gctgcgaggg aggctgcggc
     3001 aatgccttta cccagagagg atacgacccc gccggtcaca aaaatatagt tcgttgtcat
     3061 gctgaacctg agaagttagg ttgaaaagac gatggaataa ccaggacggg aaagcagtat
     3121 acccgaacat gacctgtgcc acaaactttc attatccctc ctcttcgcca gcgcactatt
     3181 gaaatcagga gtgagaaaat agcccctttg gggtaaatgt ttttgacgca aatcaagcgc
     3241 ttgtcattta aaaaatgaca caaatggcac ttgaccgcgt aattccctta gagatcaatt
     3301 tcctgccgtt ttacctgttg ccagacttct tccattgttt cgaggtcaac acctgtcatt
     3361 tccagtccac gcgcggcaac aatacgctcc acttcgcgaa aacgacgctc gaatttttcg
     3421 ttcgcttttt gcaatgcgat ttctgctttc gtccctaaat ggcgagccag attaaccgtg
     3481 gcaaacagca ggtcccccat ttcctcctcc agtttagcct ggtcgacaac agcctgccgc
     3541 gcttcgtaca tcacctcgtc gatctcttcg tagactttat caaccaccgg gccaagcgtc
     3601 gtccagtcga agccaacgtt ggcgcaacgt ttctggattt tttgcgcccg cattaaagcc
     3661 ggtaaactac gaggaatatc gtccagcgcc gaatgctgcg ctttctgcgc gcgctcttcg
     3721 gttttgattt gctcccaacg ggcaagcact tcactactgt tttcggcaga actatcagca
     3781 aaaacatgcg gatggcgacg ctctaattta tcgctaatag cagcgcaaat atcattaaag
     3841 tcaaagcgcc cttcttcctg agccatttgc gcgtaaaaca ccacctggaa tagcagatcg
     3901 cccagttcac cgcgaagatc gtcaaaatct tcacgggcga tggcgtccag cacttcgtag
     3961 ctttcttcaa gggtgtaagg cgcaatggtg gcaaatgtct gctctttatc ccacgggcag
     4021 ccgttttccg gatcgcgcag gcgctgcata atagtgagca aacgatcgat ttgattcatt
     4081 gaattgtcct gaaaattgcg ggtctgtcag gtggaaacct gtgaccagaa tagaagtgag
     4141 ttagtaacac tacccaatca gtacgttaat tttggcttta atgagttgta attcctctgg
     4201 ggcaactgtt cctttcttcg ttgctcctct tgcccgccag gcgattcttt ttacctgatc
     4261 agctaacgct acgccatcac gttcctgacc ggataaaaca acttcgaacg gatatccttt
     4321 tgattgcgtt gtacaaggaa cacacagaca catacctgtt ttgttgttgt acatgaaagg
     4381 actcaggaca acagctggac gatgtccagc ttgctcgcta ccttttgtcg ggtcaaaatc
     4441 aacccaaatc agatcgccca tatcgggtac gtatcggctt accattacca gacttcctta
     4501 tctttcggct ctccccagtc gatattctcg tggaggtttt ccggcgtgat gtcgttgacc
     4561 agttcagcaa gcgtaaatac gggctcttta cgcactggct caataattaa tttgccatcc
     4621 accaggtcaa tcttcacttc atcatcaata ttcagattga gcgcctgcat taacgtagcc
     4681 gggatccgca ccgccggtga atttccccaa cgctttacgc tactgtggat catcaccctt
     4741 tcctcaaacc gctatcatat gtagatacag tatatatcaa tctacattgt agatacgagc
     4801 aaatttctgc ctaactcccg tgcaaccgac gcgcgtcgat aacatccggc acctggttga
     4861 gtttacccag cacgcggccc agcacctgca ggttgtaaat ctcaatggtc atgtcgatgg
     4921 tcgccagttg ctgtttggtg tcgctacggc tggcaacgcc aagtacgttc accttctcgt
     4981 tggcgagaat ggtcgtgata tcacgtaaca acccactacg atcattagcc accacgcgga
     5041 ccaccagcga atatccggcg gagtagctct caccccatac cgcgtcaaca atgcgttctg
     5101 gcgcatgaga gcgcagttcc gccagttgtt cgcaatcggc gcggtgtact gaaataccgc
     5161 gcccctgggt aatgaagccg acaatctcat ctccaggaat cggctggcag cagcgcgcga
     5221 tgtggtgcat caggttgcca acaccttcga ctaccacgcg accgttatct ttactgcggt
     5281 tttgcggcgt gtagcttttt tgctgaagtt gcttcagcgc ggcggcgtcc tgctcttcgg
     5341 cactcggctt attaaattgc gattgcagga agttcaccat ctgattgaga cggatatccc
     5401 cgccaccaat cgccgccagc aactcgtcga catcattaaa gttgtaacgc ggcagcagat
     5461 gtttttctgc ttctttcagg ctgatcccca gatgttccag ctcgtcgtca aggatttgcc
     5521 gcccagccag aatgtttttg tcacggtcct gtttacggaa ccaggcgtga attttcgaac
     5581 gcccacggct ggttgtgacg taaccgaggt ttgggtttaa ccagtcacgg ctggggttcg
     5641 gctgtttctg ggtgataatt tcaatctggt cgcccatctg cagctggtag gtgaacggca
     5701 caatgcgccc gccaattttt gccccgatgc agcggtgtcc gacatcactg tggatgtggt
     5761 aagcgaagtc cagcggcgtt gatcccgcag gcaaatcaac gacatcacct ttcggcgtaa
     5821 agacatacac ccggtcgtca aagacctggc tgcgtacttc gtcgagcatt tcgccggaat
     5881 cagccatctc ttcctgccac gcaatcagtt tacgcagcca ggcaatccgg tcttcatgtc
     5941 ccgaacgtgc gccgccagct gccgcgccct ctttatattt ccagtgcgca gcaacaccca
     6001 actctgcatc ttcatgcatc tgtttggtgc ggatttggat ctcaacggtt ttgccacctg
     6061 gccccagaac cacggtatga atagactgat aaccgtttgg tttcgggtta gcgacataat
     6121 cgtcaaactc atccggcagg tggcgatagt gagtgtgcac tatccccagt gcggcataac
     6181 aatcctgtaa acgctcggcg acaatacgta ccgcacgcac atcaaacagc tcatcgaagg
     6241 cgaggttctt tttctgcatt ttgcgccaga tgctgtagat gtgtttcgga cggccataca
     6301 cttcagcttt aacgccttca gctttcatct cagcgcgcag atgaccgaca aactcttcga
     6361 tatagtgttc gcggtcgaga cggcgttcat gcagcagttt tgcgatgcgt ttgtattcgg
     6421 tcgggtgcag ataacggaag cagtaatctt ccagctccca tttcagttgc ccaatcccaa
     6481 gacggttcgc caacggcgcg tagatattgg tgcactcttt tgccgccagt acgcgttcat
     6541 cttccggcgc atcttttact tcgcgcagat gagcaatacg ctccgccagt ttgatgacca
     6601 cgcagcgaaa atcatcgacc atcgccaata acatccggcg aacgttatcg acctgttcgg
     6661 aggaaacaga atcagtgtgc gtcgctttca gctggcggat cgccgccata tcacgcacgc
     6721 cgtgaataag gttaacgaca gacttaccga cgctctcacg cagcacatct tcgctgacta
     6781 cgttggcatc ggccagaggg aacagcagcg ccgcccgcag cgtgtcaatg tccatactta
     6841 atgtcgagag gatctccacc atctcaacac cacgccacaa taacagactg gcatccggat
     6901 gcccctgcgt ctgctgcaga caatacgccc aggtttcggc taagcactca cacgacttct
     6961 ggctggtaat acccagactt gcgatccatt tttccggatc aaattcacca gccttattga
     7021 tatgtgcact tcttaccgca accattgtcc tctcctttag ggaccagacc tgccgaaatc
     7081 ggcaaatcgt aactatttaa cgcgcgagaa aagtaccatc gattccagat gtcccgtgtg
     7141 tgggaacata tccagcatcg ccagtcgcgc aatggtatat cctgctttta ataacgcttc
     7201 gctatcccga gccagcgttg cagggttaca ggatacataa actatacgaa taggttccag
     7261 ttttataatt tgctgcataa cacctgcggc acctgctcgc gccgggtcca gcaacacttt
     7321 atcgaagccg tttttcgccc acggctgctt tgtgacatct tcttcaagat tttcgtgata
     7381 aaacgtcaca ttctgtaagc cattaagacg cgcattctgc tggccttttt ccaccagcgc
     7441 cggaacacct tctacaccga ccacactggc agcttgtgtc gccaatggca gtgtaaagtt
     7501 gcccataccg cagaacagat ccagtacgcg atcttcaggt tgcacatcca gccattccaa
     7561 cgcactcgct accatttttt ggttcacacc cgcattgacc tgaataaaat cgcgcgggct
     7621 aaaagttaag cgcaacccgt tagagtcata ccagggcatc tcaccagaga cggtttcgag
     7681 tatctcacta tcgggggcga gatacagatc caggccttca gaatgcgaaa agcgttccag
     7741 tttttcgcga tctgccgaac ttagcggtgc ggtatggcgc aaaatcatca gcgtgccgct
     7801 ggttgcctgt accagttcaa catgaccaag atggcgcatt gcttgtaagc tgcccaggca
     7861 tgccctgact ttgggcagca atgcttcaag ttggggcact aaaatgggac attgtttaac
     7921 gtcgacaatg tcactggagc ccgctttgcg aaaccccatc tgaagttgct gtgttttcgg
     7981 taagtagttc agacttaaac gcgcgcgacg gcgatagccc cagggaacat cggcgatcac
     8041 ttcagagact tcgtgtttca ttaatcgggc gagtgccgca cttttgcttc gctgctgtaa
     8101 atccacgctg gcgtgttgtt gctgacagcc gccgcatacg ccaaaatgag gacagcgtgg
     8161 cgtttcgcgt tccgggctat cgcttaaccg gcgtacgact ttagcgcggg catactgttt
     8221 tttatcttca gtaacagtaa cttccgcgtt ttcctgcggc aataatccgg ggataaatag
     8281 cgttttgccg ttatgtcgcg ccacgccctg accaaaagag tcgaggtcgt tgactgaaac
     8341 ggttatgatc tgacgcgtcg tcgtgcgtcg ttttgcagag tagaattgcg ccattggcga
     8401 gactttctca atttaacagt gtgaccttaa ttgtcccata acggaactcc atgaccaact
     8461 acagcctgcg cgcacgcatg atgattctga tcctggcacc gaccgtcctt attggtttat
     8521 tgctgagtat atttttcgtc gtgcatcgct ataacgactt gcagcgtcaa ctggaagatg
     8581 caggtgccag cattattgag ccgcttgcag tttctactga atatggcatg agcctgcaaa
     8641 atcgcgaatc tatcggtcag ttaataagcg tactgcatcg tcgccattcc gatattgttc
     8701 gcgcgatttc ggtttatgat gaaaataacc gactctttgt cacctccaat tttcatcttg
     8761 atccctcatc aatgcagctc ggcagcaacg tgccgtttcc tcgccagctc actgtcactc
     8821 gtgacggcga tattatgatc ctccgcacgc cgattatttc tgagagttac tcccccgacg
     8881 aatcgcccag tagcgatgcc aaaaatagtc aaaatatgtt gggatatatt gcgcttgagc
     8941 tggatcttaa atcggttcgc ttgcagcaat ataaagagat ctttatttcc agcgtgatga
     9001 tgctgttttg tatcggtatt gcgcttattt ttggctggcg cttaatgcgc gatgtaaccg
     9061 gtccgattcg caacatggtg aataccgtcg accgcatccg tcgcgggcaa ctcgacagcc
     9121 gggtggaagg atttatgctc ggcgagctgg atatgctgaa aaacggtatc aactcgatgg
     9181 caatgtcgct ggctgcttat cacgaagaga tgcagcacaa tatcgaccag gcgacgtccg
     9241 atctgcgtga aacgctggag cagatggaaa ttcagaacgt tgagttggat ctggcgaaaa
     9301 agcgcgccca ggaagcggcg cgtattaaat ccgagtttct ggcaaatatg tcacacgagc
     9361 tgcgtacacc actgaatggt gttattggct ttacccgcct gacgctgaaa acagaattaa
     9421 caccaacgca gcgcgatcac ctgaatacga ttgaacgttc ggcaaataat ttgctggcaa
     9481 ttattaatga tgttctcgac ttctcgaaac tggaagcagg taagctgatt ctggaaagta
     9541 ttccattccc actacgcagc acgctggatg aagtcgttac tctgctggca cattcttctc
     9601 acgataaagg gttggaactg acgctcaata ttaaaagcga cgtgcctgat aacgtgatcg
     9661 gcgacccact gcgattacag caaatcatca ctaacctggt ggggaatgca attaaattca
     9721 ccgagaatgg caacattgat attctggtag aaaaacgtgc gctgagtaat accaaagtgc
     9781 agattgaagt gcagattcgg gataccggca ttggtattcc tgaacgcgat caatcgcgct
     9841 tattccaggc cttccgacag gctgatgcca gtatttcccg ccgtcatggt ggcaccgggc
     9901 tggggctggt gattacacaa aaactggtta atgaaatggg cggcgatatt tcgttccata
     9961 gccagccgaa tcgcggttca actttctggt tccacattaa tctcgatctg aacccgaaca
    10021 ttattatcga agggccatcc acccagtgcc tcgcaggtaa gcgcctggcc tatgtcgaac
    10081 caaactccgc agcagcgcaa tgcacgctgg atattttaag tgaaacgccg ctggaagtgg
    10141 tttatagccc aacgttctcc gcgctgcctc ccgcgcatta cgacatgatg ttgttaggca
    10201 tcgcggtgac cttccgcgag ccgctaacaa tgcaacatga gcgattagcg aaagcggtat
    10261 cgatgaccga tttcctgatg ctggcacttc cttgccatgc acaagtcaat gctgaaaaac
    10321 tcaagcaaga tggtatcggc gcgtgtctgc tgaaaccatt aacacctaca cgcctgttgc
    10381 ctgccctgac ggaattttgt catcacaaac aaaacacgct tttgcctgta accgatgaaa
    10441 gtaagctggc aatgacagtc atggcggttg atgacaaccc cgctaacctg aaacttatcg
    10501 gcgcattgct ggaagatatg gtgcaacatg tggaactttg cgatagcggg catcaggcgg
    10561 ttgaacgggc gaaacagatg ccgttcgatt tgatcttaat ggatattcaa atgccggaca
    10621 tggatggcat tcgggcctgc gaactcatcc accagctccc ccatcagcaa caaacgccgg
    10681 ttatcgcggt aacggcgcat gcaatggccg ggcaaaaaga gaagctgctt ggcgcaggga
    10741 tgagcgatta tctggcgaaa ccgattgaag aagagcgatt gcataatttg ttgttgcgct
    10801 acaagcctgg cagcggtatt tcctctcgcg tcgtgacgcc cgaagtcaac gaaattgtgg
    10861 tgaacccgaa tgcgaccctc gactggcaac tggcactacg ccaggcagca ggaaaaaccg
    10921 atttagcgcg cgatatgctg caaatgttac tcgatttcct gcctgaagtt cgcaacaaag
    10981 ttgaggaaca gctggttgga gaaaacccgg aaggcctggt tgatttgatt cataaactgc
    11041 atggcagttg cggctatagc ggtgtgccgc gtatgaagaa tctctgccaa ctgatcgaac
    11101 aacagctacg tagcggtact aaagaagaag atttggagcc ggagctgctg gaactgttgg
    11161 acgagatgga taatgtcgcg cgcgaagcca gcaaaattct cgggtaacgg acgtcttgtc
    11221 gagtagcaat cggcttttta tgacgctggt ttcagattgg tgacaaagtg cctgtgttta
    11281 tgccggatgc ggcgttaacg ccttatccgg cctacaaaac caattaaatt caatgaatta
    11341 aaaaatgatg tagacctgat acatagcaca acaggctatt ttgcgtttag catcagtctc
    11401 aagccggctc cagatagagc cggttttggt tttctgtctt aacgcaccat gcacgggcgc
    11461 ttgttatcga acgtccagcc aggaatcaga tactgcattc ccatcgcatt gtcacgcgcg
    11521 caaagcccgt gtttctgata cagctcatgg gctttcatca cttgatccat atcgatttct
    11581 acacccagcc ccggtttttc cggcacctga accagaccgc ctttgatctc aaacggttct
    11641 ttggtcaagc gctgattgcc ttcctgccaa atccagtgcg tatcaatcgc ggtgatttta
    11701 cccggtgcag cggcggcaac atgggtaaac atcgccaggg aaatatcgaa gtggttgttt
    11761 gagtgtgaac cccaggtcag gccaaattca tggcacattt gcgccacacg taccgaacct
    11821 tgcatcgtcc agaaatgcgg atccgccagc gggatatcaa cggattgcag ggagagcgta
    11881 tggcccatct gccgccagtc ggtggcgatc atattggttg cggtcggcag gccagttgcg
    11941 cgacggaact ctgccatcac ttcacgcccg gagaaacctt gctccgcacc acacggatct
    12001 tctgcataag ccagcgaccc tttcaggtat ttaccaattt taatcgcttc gttcagcgac
    12061 caggcaccgt taggatcgag cgtaatacgc gcctgcggga agcgttgcgc cagtgccaca
    12121 atagactcgg cctcttcttc cccggccagt acaccgcctt tcagtttgaa atcgttgaag
    12181 ccatattttt catacgccgc ttccgccagg cgcaccaccg catccggcgt catcgcttct
    12241 tcatgacgca ggcgatacca gtcgcatgag tcatccggct ggctttgata cggcagcggt
    12301 gtggctttgc gatcaccgac gaagaacaga taaccgagca tttcgacttc gctacgctgt
    12361 tgaccatcgc ccagcaggga tgccacgttt acccccagat gctgccccag cagatccagc
    12421 attgccgctt ctatcccggt aactacatga atagtggtac gcaaatcaaa tgtctgcaaa
    12481 ccgcgcccac cagcatcacg atcggcaaaa gtattacgca ccagcgtcag aacgtttttg
    12541 tattcaccca gcgttttacc taccaccagc ggaatcgcat cttccagcgt tttacggatt
    12601 ttctcgccgc cgggaatttc ccctacgcca gtgtgaccag aattatcttt gataatcata
    12661 atattacgcg taaagaacgg tgcgtgtgca ccactcagat tcatcagcat actgtcatga
    12721 cccgccaccg ggatgacctg catttcagta acaacaggcg tcgtaaattg agaactcata
    12781 tttatgtcct ttttcagaat caatgacggc cgaaaacggg acgtttacgg tcaaaggtcc
    12841 agccggggat caggtactgc atcggacctg cgtcgttacg cgcaccgcca ggcagacgtt
    12901 tataggcctc atgtgccttt tgtacctgtt cccagtccag ttccacgccc agaccgggcg
    12961 catcaggaac ggcaattttt ccgtttttaa tctccagcgg atttttggtc aggcgacaat
    13021 cgccctcctg ccaaatccag tgggtatcga tagcggtagg attacccggt gccgccgcgc
    13081 ccacatgggt aaacatcgcc agagagatat cgaaatggtt attagaatgg cagccccagg
    13141 tcagccccca gtcgtcgcaa agctgcgcca cacggactgc accggaaagc gtccagaagt
    13201 gcggatcggc aagtggaata tctaccgcat tgagcatcac cgcatgaccc atttcgcgcc
    13261 agttggtggc gatcatgtta gtcgcgacgg gcaagccggt cgcccgccga aactccgcca
    13321 taacttcacg accggagaaa ccctgttctg cgccgcatgg atcttcggca taggtaagaa
    13381 catcattcag ccctttgcat agagaaatgg cttcatcaag tagccaggca ccgttgggat
    13441 caacggtaat ccgcgcatca gggaagcgtt tcttcaatgc acgaacagtg tcgatttctt
    13501 gctcgccagg taacaccccg cccttaagtt tgaaatcttt aaagccatag cgatcctgtg
    13561 aggcttccgc cagacgcaca acggcttcgc tgttcatcgc tttctgatgg cgcaactgat
    13621 accactcatg gttgcccggc gtattttcca gataaggaag atcggttttg gtccgatcac
    13681 cgatataaaa cagataaccg aggacggtaa tagtctcgcg ttgcttgcct ggccccaaca
    13741 gttcgcagac cggaacattc agcgccttac ctagcaggtc aagcaaggcg gcttccagcg
    13801 ccgccacggc attaacgtgc aattcaaaag tccaggcacc tttgccgaag gtatcaaaat
    13861 cggctgcctg attaccttta tgcacctgct gaaccacttt attcaggcgc gcaacttcct
    13921 ggcccagaac catcggaata gcatcgacca gtgtttgata aatcacctct ccgcccggcg
    13981 cttcaccaat gccggtatgc ccggcgttat cggtgagtac cacaatattg cgggtgaaat
    14041 atgcgttatg tgccccacca atattaagca acatgctatc gtgcccggcg accggaatga
    14101 ctttcatatc agtaataaca ggactggatt gtgtcgtcat cttattgccc cgcgacaggt
    14161 ttcagctcga tacgcttgat atcgcccacc agcaccaggt agctcagtac cgcgattaag
    14221 gcatgaacac caacataaat cagcgcccca ttaaaggagc cagtcgtgcc aacgatataa
    14281 ccaattgcga ttggcgtgac gatgccagaa atgttaccga acatgttgaa caggccaccg
    14341 gaaagaccgc tgatctcttt tggcgcggta tctgccatta ctgcccaacc cagcgccccg
    14401 atgcccttac cgaagaaggc cagcgccata aagccgatga tcatccactc aacgttgacg
    14461 tagttgcaga acaccatcac catcgacagc aacatgccca ttacgatcgg tgttttacgc
    14521 gcaatgttca gcgatcccgt gcggcgcatc agccagtcgg aaataatccc acccagcaca
    14581 ccgccgataa aaccgcaaac cgccggaacg gaagccacaa agcccgcttt cagaattgac
    14641 atcccacgcg cctgcaccag ataaaccgga aaccaggtaa tgaaaaagta agtcagggcg
    14701 ttgatacagt actgaccgat ataaacgccg atcatcatcc gtgacccgag cagttgtttg
    14761 atctgccccc acttcacgct gaacggcact ttaactttgg tgttttgctg atccatattg
    14821 atcagcgcgc cacccgcggc gatgtactcc agctctttct gatttacccc cggatgttga
    14881 tttggctcgt ggatgacttt caaccagata aagctgatga caatccccag accgcccata
    14941 aagaagaaga cgtgtgacca gcccacttca tgcgtcagcc agcccataat cggcgcgaag
    15001 atcaccgttg cgaagtattg agcggagtta aaaatcgaca ccgccgttcc cctttcctgc
    15061 gccggaaacc aggccgcaac aatgcgactg ttgccgggga aagatggcgc ttcagcaagc
    15121 ccgaccagga agcgcagagt aaacagggca acgataatgc cgaatccact aaagatatcg
    15181 acgaagcctt gcagcaaggt aaacatcgac cagataaaga tcgaccagaa gtagacgcgt
    15241 tttgaaccaa aacgatccag caaccaacca ccagggatct gcccgataac ataagcccat
    15301 gagaaggcag agaacacata gcccattccc acgggatcaa ggccgatatc tttggccatt
    15361 tccgaaccgg caatagaaag cgtagcgcgg tcgccgtagt tgaaggatgt gacgataaac
    15421 aacatcacca ctatccagta acgagcattc gtgcgttttt ccacactgct cgcagcctga
    15481 cttaaagaac tcgttgttgc actcctgaaa attcgcgtta gccacgctaa ctctggactg
    15541 cgacatagcc aggaaatcag aggtgacgta gggtgttttt tgccgttttt ataggtcgtt
    15601 cgccgaatac ggcgcgtgtt tatatctggc aatagcagta taaaaagcgc gccatagcgg
    15661 ctcaccgtgc aacaacacaa cattaatgcg ttcaatgagg cccgattttg gcattagccc
    15721 tggacggtgg aatccacttc acggaaatga aaacaagaac aagaaaggaa gggttaaaac
    15781 gaagaaataa agaagagtat gaaatggatc gcttgactcc aggcaaacgc cagtaaaaat
    15841 ccgcgctatg aagcagtttt tactggcatt tgcctgaaaa gattcgattc agcaccgcta
    15901 aaacgacatt taccgctcgc tgaacatatc aggacaacag cgtgccccac tgttcgaccc
    15961 acggatttga ttccgtttcc ggttccgggt tctcactggc gtcaatcaac agcatttcgc
    16021 caactcgctg agcgctctgt tcctgcaaca aggcatcgaa ctgtttgccg ccattgcaga
    16081 aatttacata actgctgtcg ccgagcgcaa tcacgccata acgcagattc ggctggaagc
    16141 ccagactatc tttgattccc tgaaagagcg gcacaatgct atcaggaagg tcgccctgcc
    16201 cggtcgtgga cgtaaccacc agaacatact tatcctgata aggcagccag tcgcttaatt
    16261 caggatcttc aaataccgtt gctttgtggc cctgtgcggt cagaattgct tctgcttctt
    16321 ccgccactaa cagcgaattt ccgtacatgg tgccgacaaa aataccaatt tccgccatac
    16381 cgttttctcc ctggattagg aacttatctc tccatcctga cccgatggcg cactgaactc
    16441 aaccctttca ttttcaggaa gcagaccgcg ccagccaaat tgtgataacg cctgcatcca
    16501 ggtgtcgtcc aggcccgcgt gaatagtcag cggctcgcca gtaaaaggat gcgtcaatga
    16561 caactgactg gcatgtagca ttaaccgctg gaggccaaaa tgctcagcac cgctgcgatt
    16621 ctggcgtaaa tcgccatgtt tgctatcgcc aataatcgga tgacgcaaat gggcaagatg
    16681 tcggcgaagc tgatgtttgc gtccggtttt cggctccagt tccaccaggc cgtagcgcgt
    16741 ggtcgggtaa cgtccggtcg ctaccggcat ttctacggtc gccagaccgc gataatgcgt
    16801 cactgctggc tgcgggcctt tatcttcgcg ggcaaattta tcagcgattt tgtccagttc
    16861 ttccaccagt ggataatcca gcaccgcttc ttccatcaac cagccgcgca caatcgcatg
    16921 gtaacgtttc tggatttggt gctgttcaaa ctgttgtgcc agcagccgtc cggcctcgct
    16981 ggataatccc atcaacaaca caccagaagt gggtcggtcc agacgatgag cagtaaaaac
    17041 atgctgtcct atctggttac gcacggtttg catgaccact actttctcgt cgcgatccag
    17101 ccagctacgg tgaaccagcc agccggaggg tttatttacc gaaaccagcc attcatcctg
    17161 atagagtatt tccagcatta gctcgcatca tccgcaaaaa gagcatccag tttttccaac
    17221 tcagccagaa taagcgcgtg ttgcggatgg tccgtcgcca gcgccatttc ataatagggt
    17281 gcaacagcaa aagcgcccgg taacggctgg ttgttattta acaaatcgtg cattcgcggg
    17341 atcagcaccc actgcaacca ctccagtggt tccatggtgt ccataaagaa cggttgggta
    17401 ctattaaatt gatgcggctg gggttcatcg tttcgccagt gctgatgttc acgcagtaac
    17461 gcttcaagcg cctgcaactg gagacgaacg cggtcatgag tggtcataaa gcaacctcaa
    17521 taagaaaaac tgccgcgaag gatagcagga ataaaaaaag ggagcactgt attcacagcg
    17581 ctcccggttc gtttcgcagc attccagcta cttttgttgc tccctgctca tccttgacaa
    17641 cttttcctct ggccttgcgg ccaatcgttc atcctgaact attgcttcct gctcacacca
    17701 ccccgatgtg atacttcatc ctgaagtgtc cttggccttc ctgaccagcc gaatcatcct
    17761 gaccggttct cattctccat cctggaggtg tcctttaacg cgtcctgcgt catcctcttc
    17821 gcttcatcca gaagcctttc cctgaaacac catcctggtg tgtcctgcag aagtgtcatc
    17881 atcctgatgt tcacttcgtt gtctgactcc ctgtcgacga agatagaatc gtctttttcg
    17941 cgaagtctta caaggcgctt acagacaatg ccttaaggaa aatttcatat gaaagttaag
    18001 aattaacaat caacattttg attttaaatg aaaataacga ttcttatctt tttaagtatt
    18061 cgcttatttc ctggcgatct cctacagagt ctgtaagaga tctctcacaa ccagatgggg
    18121 tatttggatt acagaagagg cttaagttga ttaaggaatt ccgcaagatt tgaggctaaa
    18181 tgcgtccgtt tacgtgttcc cagcgtctct ttgcacactt caccggagag attacacacc
    18241 gagatgacct ctaactcttc ttccagcgtc gcgataaata gcgtgggcgg cagttttagc
    18301 cttttctgag tcaccagatg accaatcaga ttttcctgca cccgacggaa gtcgtcttca
    18361 ctccaggttt gcagcaatgt cagtttgata tcgccaaact gcgcgtgcat atccccggca
    18421 aactgagtcg tataaaaggt atgaattgtg ggttgtatca caatatcaaa agcgcgttca
    18481 accgcgttta cattttgttc cccggtaaac ggctggggtt gccagtatac ggcatcttcg
    18541 gtagttgaaa taatgcacgg tgaaggaaca ccgtacagtt cctcacttaa cggccagctt
    18601 ttatgctctt catgccacgc atcgcagtag cgtgcagtaa aatctttcag ggcttgtgcg
    18661 gtcaaatcgt ccactaattt ctctcttcac ttaaaccaga tacacttgtc gtttagttta
    18721 tctggtttat gacggtgaaa catgtcttct tatgcaaacc atcaggcact tgcgggcctg
    18781 actcttggaa aatcaaccga ttaccgggat acctatgacg ccagcctact gcaaggcgtt
    18841 ccacgcagcc tgaatcgcga cccgctgggt ctgaaagcgg ataacctgcc ttttcacggt
    18901 acggatatct ggacgctgta tgaactttcc tggctgaatg cgaaaggttt gccgaaggtc
    18961 gctgtcggtc atgttgaact tgattacacc agcgtaaatc tgattgagtc gaagagtttt
    19021 aagctctatc tcaacagttt taaccagacg cgttttaata actgggatga ggtgcgccag
    19081 acgctggagc gcgacttaag cacttgcgct cagggtaaga ttagcgtggc gttatatcgt
    19141 cttgatgaac tggaaggcca gccgataggt cattttaatg gcacttgcat tgatgaccag
    19201 gatatcacta tcgataacta tgaattcact actgactatc tggagaatgc cacctgtggt
    19261 gaaaaagtag tggaagagac gcttgtcagc cacctgctga aatcaaactg cctgatcacc
    19321 catcaaccag attggggttc gatccaaatt cagtatcgtg gacgccaaat tgacagagaa
    19381 aaactgctgc gttacctggt ctcattccgt catcacaacg agttccacga acagtgcgtg
    19441 gaacgcatct ttaatgacct gttacgcttc tgccagccag aaaaattgag cgtttacgca
    19501 cgttataccc gtcgtggcgg tctggacatt aacccgtggc gcagtaatag cgattttgtc
    19561 ccatcgacca caagactggt tcggcaataa attttttctc aattttgcgt gctggattca
    19621 cgcagaaggt tgtgaaaggt catcaggcag ggctattgta atcaaaggga atgacgatat
    19681 tcgtcccata aggagttttt cttgattaca catattagcc cgcttggctc catggatatg
    19741 ttgtcgcagc tggaagtgga tatgcttaaa cgcaccgcca gcagcgacct ctatcaactg
    19801 tttcgcaact gttcacttgc cgtactgaac tccggtagtt tgaccgataa cagcaaagaa
    19861 ttgctgtctc gttttgaaaa tttcgatatt aacgtcttgc gccgtgaacg cggcgtaaag
    19921 ctggaactga ttaatccccc ggaagaggct tttgtcgatg ggcgaattat tcgcgctttg
    19981 caggccaact tgttcgcggt cctgcgtgac attctcttcg tttacgggca aatccataac
    20041 accgttcgtt ttcccaacct gaatctcgac aactccgtcc acatcactaa cctggtcttt
    20101 tccatcttgc gtaacgctcg cgcgctgcat gtgggtgaag cgccaaatat ggtggtctgc
    20161 tggggcggtc actcaattaa cgaaaacgag tatttgtatg cccgtcgcgt cggaaaccag
    20221 ctgggcctgc gtgagctgaa tatctgcacc ggctgtggtc cgggagcgat ggaagcgccg
    20281 atgaaaggtg ctgcggtcgg acacgcgcag cagcgttaca aagacagtcg ttttattggt
    20341 atgacagagc cgtcgattat cgccgctgaa ccgcctaacc cgctggtcaa cgaattgatc
    20401 atcatgccag atatcgaaaa acgtctggaa gcgtttgtcc gtatcgctca cggtatcatt
    20461 atcttccctg gcggtgtggg tacggcagaa gagttgctgt atttgctggg aattttaatg
    20521 aacccggcca acaaagatca ggttttacca ttgatcctca ccggcccgaa agagagcgcc
    20581 gactacttcc gcgtactgga cgagtttgtc gtgcatacgc tgggtgaaaa cgcgcgccgc
    20641 cattaccgca tcatcattga tgacgccgct gaagtcgctc gtcagatgaa aaaatcgatg
    20701 ccgctggtga aagaaaatcg ccgcgacaca ggcgatgcct acagctttaa ctggtcaatg
    20761 cgcattgcgc cagatttgca aatgccgttt gagccgtctc acgagaatat ggctaatctg
    20821 aagctttacc cggatcaacc cgttgaagtg ctagctgccg acctgcgccg tgcgttctcc
    20881 ggtattgtgg cgggtaacgt aaaagcagtc ggtattcgcg ccattgaaga gtttggtcct
    20941 tacaaaatca acggcgataa agagattatg cgtcgtatgg acgacctgct acagggtttt
    21001 gttgcccagc atcgtatgaa gttgccaggc tcagcctaca tcccttgcta cgaaatctgc
    21061 acgtaatctc ccctcccccg gtgacgtttg ttgccgggtt ttctcgtttt tggtcactta
    21121 ctcatcaact catttcattt gttatatgaa tgtttcttac caccctcacg cgacaaatat
    21181 catcacagtt aatatgtcat acaatttatg ttgcaacgca aacgtttctc tattttcata
    21241 aaccgtattt tatcgctatg aaaaagaaat tatcgccatg attaactaaa agtattgatt
    21301 ttttcagttc aacctacata tattgcgcgc cctggaagaa gtcagatgtc gtttaatggg
    21361 caaatattgc ccttaaattc tcttttactt ttgatttaca gagtaaagcg ttgggataat
    21421 ctatcttcca agtagattat tgtatttgag atcaagatca ctgatagata cataacttgt
    21481 gtgtatcttt ccgccctcaa attattacgg cggtaaatga ttaagccatc gccgatagac
    21541 agatttcatt tttacggtca ggcaccttcc cgggctgaac tggctaaaag ctgaattatt
    21601 tgcattcctc caggagaaat agatggaaac gactcaaacc agcacgattg cgtcgaaaga
    21661 ctctcgtagt gcctggcgca agacagacac catgtggatg ctgggccttt acggcacggc
    21721 aatcggcgcg ggcgtgctgt tcctgccaat caacgccggt gttggcggta tgatcccgct
    21781 gatcatcatg gctatccttg cgttcccgat gacgtttttt gctcaccgcg gcctgactcg
    21841 cttcgtactg tctggtaaaa acccgggcga agacatcacc gaggttgtag aagaacactt
    21901 tggtattggc gcaggtaaac tgattaccct gctctacttc ttcgctatct acccgatcct
    21961 gctggtttat agcgtggcaa tcaccaatac agttgaaagc ttcatgtctc accagctggg
    22021 tatgacgcca ccgccgcgtg cgattctgtc gctgatcctg atcgtgggta tgatgaccat
    22081 cgttcgcttc ggtgagcaga tgatcgttaa agcgatgagt attctggtgt tcccgtttgt
    22141 tggcgtattg atgctgctgg ctctgtacct gatcccgcag tggaacggcg cagcactgga
    22201 aacgctgtct ctggacactg catctgcaac cggaaacggt ctgtggatga ccctgtggct
    22261 ggcaattccg gtaatggtgt tctcgttcaa ccactctccg atcatctctt ctttcgccgt
    22321 tgcgaagcgt gaagagtacg gcgatatggc agaacagaaa tgctcgaaga tcctggcatt
    22381 cgcacacatc atgatggtgc tgaccgtaat gttcttcgtc ttcagctgtg tactgagcct
    22441 gactccggca gacctggctg cggctaaaga gcagaacatc tcgattctgt cttacctggc
    22501 taaccacttt aacgcaccgg ttatcgcgtg gatggctccg attatcgcga ttatcgctat
    22561 caccaaatcc ttcctcggcc actacctggg cgcacgtgaa ggcttcaacg gtatggtgat
    22621 taaatctctg cgtggtaaag gtaagtctat cgaaatcaac aagctgaacc gtatcactgc
    22681 gctgttcatg ctggtaacga cctggattgt tgccaccctg aacccgagca tcctgggtat
    22741 gattgaaacc ctgggcggtc caatcatcgc gatgatcctg ttcctgatgc cgatgtacgc
    22801 aattcagaaa gtaccggcaa tgcgtaagta cagcggtcac atcagcaacg tattcgttgt
    22861 cgtgatgggt ctgattgcaa tctccgcaat cttctactct ctgttcagct aagtcctttc
    22921 gcgccgcttt cgggcggcgc ttcctccgtt ttaacgcgat gtatttccta tgattagcgt
    22981 attcgatatt ttcaaaatcg gcattggccc ttccagttct cataccgttg gaccaatgaa
    23041 agcgggtaaa caatttaccg acgatctgat tgcccgtaac ctgcttaaag acgtgacccg
    23101 cgtggtggtt gacgtgtacg gctcgctctc tctgaccggt aaaggccacc acactgatat
    23161 cgccattatt atgggcctgg cgggtaacct gccggatacc gtggatatcg attccatccc
    23221 tggttttatt caggatgtga atactcatgg tcgcctgatg ctggcaaacg gtcagcatga
    23281 agtggagttc ccggttgatc agtgcatgaa cttccacgcc gacaaccttt ctctgcatga
    23341 aaacggtatg cgcattaccg cgctggcggg cgataaagtc gtttacagcc agacttacta
    23401 ctctattggc ggtggcttta tcgttgatga agagcatttt ggccagcagg atagcgcacc
    23461 ggttgaagtt ccttatccgt acagttcagc agccgatctg caaaaacatt gtcaggaaac
    23521 cgggctgtca ctctctggcc tgatgatgaa aaacgagcta gcgctgcaca gcaaagaaga
    23581 gctggaacag cacctggcga acgtatggga agtcatgcgt ggcggtattg agcgcggtat
    23641 ttccaccgaa ggcgtgttgc ctggcaaact gcgcgttcca cgccgtgctg cggcactacg
    23701 ccggatgctg gtcagccagg ataaaaccac cactgacccg atggcggttg ttgactggat
    23761 caacatgttt gcactggcag tgaacgaaga gaacgctgct ggcggacgcg tggtgactgc
    23821 gccgactaac ggtgcgtgcg ggattatccc ggcagtgctg gcgtactacg acaagtttat
    23881 ccgcgaagtg aacgctaact cactggctcg ttacctgctg gtagccagtg ccattggttc
    23941 tctttataag atgaacgcgt caatttctgg tgctgaagtc ggctgccagg gtgaagttgg
    24001 tgtggcgtgc tcaatggcgg cggctggtct ggcagaactg ttaggtgcaa gcccggcgca
    24061 ggtgtgcatc gcggcggaaa tcgccatgga gcacaacctc ggtctgacgt gtgacccggt
    24121 cgccggacag gtccaggtgc catgcatcga gcgtaacgcc attgcggcag taaaagcggt
    24181 gaacgccgca cgtatggcgc tacgccgtac cagcgagccg cgcgtctgcc tcgataaagt
    24241 tatcgaaacc atgtacgaaa caggtaaaga tatgaacgcc aagtaccgcg aaacctctcg
    24301 cggcggcctg gcaatgaaga tcgttgcctg cgattaatcg ctctccagag gcctcgtttt
    24361 gcgaggcctc ttcccgattt ctcatccagc cgtagcctgt tccggcatcg aatgttaccc
    24421 ttatcgcctg atctttaagg gggttatcgt ggctgttcat ttgcttattg tcgatgcact
    24481 gaatcttatt cgtcgcattc atgccgttca gggatcgccc tgtgtcgaaa cctgccagca
    24541 tgcgctcgat cagctcatta tgcacagcca gccaacccac gcggtcgccg tttttgatga
    24601 tgaaaaccgc agtagcggct ggcgtcatca gcgtttacca gattacaaag cgggtcgacc
    24661 gccaatgccg gaagagttgc acgacgagat gcctgcatta cgcgccgcct ttgagcaacg
    24721 cggcgtcccg tgctggtcaa ccagcggcaa cgaagccgat gacttagccg ccacgctggc
    24781 ggtcaaagtg acacaggccg ggcatcaggc aacgattgtt tcgacagata aaggctactg
    24841 tcagttactt tcaccgacat tacgtattcg tgattacttc cagaaacgtt ggctggatgc
    24901 accatttatc gataaagaat ttggcgttca accgcagcag ttgcccgatt actggggact
    24961 tgcggggatc agcagttcaa aggtaccggg tgttgcggga atcggaccaa aaagcgccac
    25021 gcagctgctg gtcgagtttc agagtctgga agggatatat gagaatctgg atgcggttgc
    25081 cgaaaagtgg cgcaaaaaat tagaaaccca taaagagatg gcgtttctgt gccgcgatat
    25141 tgcccgctta caaaccgatt tgcatatcga cggcaattta cagcaattgc ggttggtacg
    25201 gtaacggcga gccggatgca aacgattatc aacacattga atttaatgca cttatatacc
    25261 ggataagacg cggctagcgt cgtatccggc attatcacat cagcgcattt accaggcggt
    25321 atggtaaagc tctacaatat cctcaagcgt tgcttcacgc gggttgccac cggtacaaac
    25381 atcatccagt gccgcctgcg ccagtgccgg aatgtcttcc ttgcgtacac caacatcacg
    25441 caaatgtggc ggaataccga catcacggtt gagagcaaac accgcttcaa cagcggcatt
    25501 acgcgcctct tccagccact ttcacgccca taacgcgcgc gatatcgcgg tacttctcac
    25561 cggtaaagtc agcgttatag cgcatgacat gcggtaacag gatggcgttc gcaacaccgt
    25621 gtggagtgtt gtaaaacgcg cccagtggat gcgccatacc atgcaccaac cctaacccaa
    25681 cattcgagaa gcccataccc gcaacatact gcccgagcgc catttcttct ccggcatcct
    25741 tatcaccagc aaccgatcct cgcagcgccc cagcaatgat ttcaatcgct ttaatgtgca
    25801 gtgcatcggt tagcgcccac gcgccacggg taatataccc ctcaatagca tgagtgagcg
    25861 catcgacacc cgtcgcagct ttcagcgctg gaggcatacc atccatcatg tcagcgtcaa
    25921 taaacgccac ctgcgggata tcatgcggat caacgcaaac aaacttgcgc cgtttctctt
    25981 cgtcagtgat cacgtagtta atggtcactt ctgccgcagt acctgctgtg gtaggaattg
    26041 ccagaatcgg tacactgggt ttattggtcg gggaaagccc ttccaggcta cgcacatcgg
    26101 caaactccgg gttgttgctg ataatgccaa tcgctttaca agtatcctgt ggagaaccac
    26161 caccaatagc gatcaggtaa tccgcgccgc tattctggaa tacaccgagc ccttctttga
    26221 cgacagtaat tgttgggttg ggcactacgc cgtcgtaaat cgcccatgcc agccctgcag
    26281 catccatctt atcggtcact ttcgccacca cgccgcattg caccagcgtt ttatcggtga
    26341 cgatcagcgc cttctgataa ccacggcgtt tcacctcatc ggttaaagcc ccaacagcac
    26401 cccgaccaaa ccatgccgtt tcgttcagaa tcattctgtt agccatcatc cttctccttg
    26461 ttgctttacg aaattactct tcaatccgta acccataggt tttgaatttc tccagcacta
    26521 cggcaatctc ttcatcgctc agcactggca ccgggtccgt aatcgccagg gtcgtcaggt
    26581 aaagttgcgc cagcacttca acttcatgcg ccagccataa cgctttttcc agattcacct
    26641 cacaagcgat aagcccatga tgttgtaaca aagttgcctt acgatttttg agagccagcg
    26701 caacatgttc agaaagttcg cgtgttccaa aggtcgcata aggcgcgcaa ggaatagaat
    26761 taccacctgc cgccgcaatc atgtagtgaa tagcggggat cggtcggtta agaatggaaa
    26821 ctgctgtgca atgaacggca tgattgtgaa caaccgcgtt ggcatccggt ctgctttgat
    26881 aggccgccat atggaaacgc cattcgcttg aggggagctt tccttcctca tgtttaccgt
    26941 taccatcaat aaaggcaata tgcgactccg tcagtttttc atatggaatg cctgtaggcg
    27001 taatcagcat cccatcctga taacgtacac tgacgttccc cgctgtcccc tggttcagtc
    27061 ccaggcgggt catttccagg caagtgtcaa taatctgacg agcaagttta tttcgttcca
    27121 ttagctacct ctctctgatt caaaacaggg caataatgtt gttcctttca cactattgaa
    27181 ttagccgttt aattacccac cat
//
LOCUS       DAJTMC010000041        26806 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.041, whole genome shotgun sequence.
ACCESSION   DAJTMC010000041 DAJTMC010000000
VERSION     DAJTMC010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26806
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.041"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(<1..326)
                     /gene="rpnB"
                     /locus_tag="OO130_001529"
     CDS             complement(<1..326)
                     /gene="rpnB"
                     /locus_tag="OO130_001529"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HCR5364987.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARDFIDIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDA"
     gene            complement(523..1296)
                     /gene="hisP"
                     /locus_tag="OO130_001530"
     CDS             complement(523..1296)
                     /gene="hisP"
                     /locus_tag="OO130_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HCR5364988.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEAHERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(1304..2020)
                     /locus_tag="OO130_001531"
     CDS             complement(1304..2020)
                     /locus_tag="OO130_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5364989.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGIYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVLHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(2017..2703)
                     /gene="hisQ"
                     /locus_tag="OO130_001532"
     CDS             complement(2017..2703)
                     /gene="hisQ"
                     /locus_tag="OO130_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HCR5364990.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFFGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(2793..3575)
                     /gene="hisJ"
                     /locus_tag="OO130_001533"
     CDS             complement(2793..3575)
                     /gene="hisJ"
                     /locus_tag="OO130_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HCR5364991.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(3796..4578)
                     /gene="argT"
                     /locus_tag="OO130_001534"
     CDS             complement(3796..4578)
                     /gene="argT"
                     /locus_tag="OO130_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HCR5364992.1"
                     /translation="MKKSILALSLLVGLSVAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(4844..5413)
                     /gene="ubiX"
                     /locus_tag="OO130_001535"
     CDS             complement(4844..5413)
                     /gene="ubiX"
                     /locus_tag="OO130_001535"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HCR5364993.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAISLPEDLFARWQGA"
     gene            complement(5508..7025)
                     /gene="purF"
                     /locus_tag="OO130_001536"
     CDS             complement(5508..7025)
                     /gene="purF"
                     /locus_tag="OO130_001536"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HCR5364994.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQDYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(7062..7550)
                     /gene="cvpA"
                     /locus_tag="OO130_001537"
     CDS             complement(7062..7550)
                     /gene="cvpA"
                     /locus_tag="OO130_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HCR5364995.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(7809..8471)
                     /gene="dedD"
                     /locus_tag="OO130_001538"
     CDS             complement(7809..8471)
                     /gene="dedD"
                     /locus_tag="OO130_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HCR5364996.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(8461..9729)
                     /gene="folC"
                     /locus_tag="OO130_001539"
     CDS             complement(8461..9729)
                     /gene="folC"
                     /locus_tag="OO130_001539"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HCR5364997.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSAKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(9799..10713)
                     /gene="accD"
                     /locus_tag="OO130_001540"
     CDS             complement(9799..10713)
                     /gene="accD"
                     /locus_tag="OO130_001540"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HCR5364998.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVEMGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPLGFQRSEFLIEKGSIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(10869..11528)
                     /locus_tag="OO130_001541"
     CDS             complement(10869..11528)
                     /locus_tag="OO130_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5364999.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGDKLFS
                     NPDSKIFRCSYLDKTHQFYERHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLIFTYAGYFFGMLPFIQSNLKLMIVGIIFVSILPGVFEFIRHKRAAARAA
                     K"
     gene            complement(11611..12423)
                     /gene="truA"
                     /locus_tag="OO130_001542"
     CDS             complement(11611..12423)
                     /gene="truA"
                     /locus_tag="OO130_001542"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HCR5365000.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPDDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(12423..13436)
                     /locus_tag="OO130_001543"
     CDS             complement(12423..13436)
                     /locus_tag="OO130_001543"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCR5365001.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(13502..14638)
                     /gene="pdxB"
                     /locus_tag="OO130_001544"
     CDS             complement(13502..14638)
                     /gene="pdxB"
                     /locus_tag="OO130_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HCR5365002.1"
                     /translation="MKILVDENMPYARDLFSRLGEVTAVPGRPIAVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQHADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            14737..15732
                     /gene="flk"
                     /locus_tag="OO130_001545"
     CDS             14737..15732
                     /gene="flk"
                     /locus_tag="OO130_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HCR5365003.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(15729..16907)
                     /locus_tag="OO130_001546"
     CDS             complement(15729..16907)
                     /locus_tag="OO130_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5365004.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPLKFALLVVGRLILGFGESQLLTGALTWGLGIVGAKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(17227..18447)
                     /gene="fabB"
                     /locus_tag="OO130_001547"
     CDS             complement(17227..18447)
                     /gene="fabB"
                     /locus_tag="OO130_001547"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HCR5365005.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMKMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            18606..20612
                     /gene="mnmC"
                     /locus_tag="OO130_001548"
     CDS             18606..20612
                     /gene="mnmC"
                     /locus_tag="OO130_001548"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HCR5365006.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVVAESGFGTGLNFLTLWQSFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
                     TSQQDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQDAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSAPWFNRTGSSKREAAIIGGGIAC
                     ALLSLALLRRGWQVTLYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(20733..21011)
                     /locus_tag="OO130_001549"
     CDS             complement(20733..21011)
                     /locus_tag="OO130_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HCR5365007.1"
                     /translation="MIAEFESRILALIDGMIDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQQ"
     gene            complement(21045..21593)
                     /gene="epmC"
                     /locus_tag="OO130_001550"
     CDS             complement(21045..21593)
                     /gene="epmC"
                     /locus_tag="OO130_001550"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HCR5365008.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(21593..22402)
                     /locus_tag="OO130_001551"
     CDS             complement(21593..22402)
                     /locus_tag="OO130_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HCR5365009.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFAGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(22402..23226)
                     /gene="mepA"
                     /locus_tag="OO130_001552"
     CDS             complement(22402..23226)
                     /gene="mepA"
                     /locus_tag="OO130_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HCR5365010.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPPGDGCGAELQSWFAPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(23230..24315)
                     /gene="aroC"
                     /locus_tag="OO130_001553"
     CDS             complement(23230..24315)
                     /gene="aroC"
                     /locus_tag="OO130_001553"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HCR5365011.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGILLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(24350..25282)
                     /gene="prmB"
                     /locus_tag="OO130_001554"
     CDS             complement(24350..25282)
                     /gene="prmB"
                     /locus_tag="OO130_001554"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCR5365012.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPDAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLLAAREYFAIYKD"
     gene            25448..25999
                     /gene="smrB"
                     /locus_tag="OO130_001555"
     CDS             25448..25999
                     /gene="smrB"
                     /locus_tag="OO130_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HCR5365013.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(26072..26785)
                     /locus_tag="OO130_001556"
     CDS             complement(26072..26785)
                     /locus_tag="OO130_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005007603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HCR5365014.1"
                     /translation="MATPDSVNYAIFKATFMPNSQPDLVSWTGDSSTQPSMSKISDSR
                     VSMSACPGLEQYDSQTKTGWTCNELKMFVYYDGNLHGCPWIVSSFVKSRDPFAKTYDD
                     DFPDYIGPTKVSSSCPAVPLAPYDVSWNENYVVHNKVVRLQSTGGVIEQTLPTFLMEN
                     GKLCNGNNFDERGVYCRFIAQQMTFSTSGCDNAKVTVTPEPQPITSRQLHDMKLRVDT
                     TSRQPIDSTCRFTYILNMY"
BASE COUNT         6612 a         7329 c         6757 g         6108 t
ORIGIN      
        1 gcatcaagat ggttttgcat tgccgcaatg gaataacgca tcatgcgaaa agccattaat
       61 tcttccggct tactttggtg ctctatcact acataaatat aacccactcc ctcctgcgtt
      121 ttcacagacc acaagaggtc ggaataatat tgccgcaggt cttcatcaat aaaactgttt
      181 ggttccagtt taagcgtcgt taaatcacac agtttgcgca gcggcgcggg aagatgaata
      241 tcaataaaat cccgcgcggt gtctggatgg cgtaaaaaag atttaaatac cgcatcatgc
      301 ggcgtggaag ttgtcgatat tgtcatggcg ttccgtcacc cctcaaattt aagatgacgc
      361 gacaataacc acgattaaca cccaatgcag caggcagttt ttatctttgg acagcgaatt
      421 cagaagtatt tttagcggac tgcaactttt acatttcagc ggaagctgac tcgcaaattc
      481 cccatgaaaa tactccggca gcgttagcca ccggagagga aattatttca gcgatccctt
      541 aaggaactgt tgcagacgag ggctttgcgg gttgccaaat aactgctccg gcgcgccctc
      601 ttcttctatt ttcccctgat ggaggaaaat gacatgagta gaaacatggc gagcaaagcc
      661 catttcgtga gtcactacca ccatggtttt cccctcttct gccagttgct gcataatacg
      721 caacacttcg cctaccagtt caggatcgag cgccgaggta ggttcatcaa acagtaaaac
      781 ttccggttcc atcgccagcg cccgcgcgat agaaacacgc tgttgctgac cgccggaaag
      841 atgcaccgga tatttcccct gcgcacgttc gtctatcccg acttttgcca gatacttgac
      901 cgcccgctcg tgcgcttcct gcttgctcag gcccaacacc tgaatcggcg cttccatgac
      961 gttttccagc accgtcatat ggctccagag attgaagtgc tggaatacca tcgtcaggcg
     1021 tgtgcgcagt aagcgcagtt gatttttatc ggcgactttg agttgaccgt ctttgtcgcg
     1081 caccagattg atcgtctggc cattgaccac gatcgaccct tcactcggtt tttcgaggaa
     1141 gttaatgcag cgcagaaagg tacttttccc cgatcccgac gatccgatga tgcttattac
     1201 atctccggca ttcgcttgca gtgagacccc tttcagcact tcatgttcgc cgtagcgttt
     1261 gtgcaaatcg ataacgttta atttattctc ggacatcgta ttctcagtgc gttgaagaag
     1321 gtttcacatg ctgcaaccag cgtttttccg ctctgcgaaa gaggctgatc aggacataag
     1381 agatgattaa atagagcacc gcggcaatgc cgaaggcggt aaaaggttga tacgtggcgg
     1441 cgttaatatc gcgggctatt ttcagcagat ccggcacagt ggccgtaaac gccagcgcag
     1501 tagagtgcag catcaggatc acttcgttgc tgtatgccgg taacgcaata cgcagcgcag
     1561 aaggcaaaat aatgcaacga tacattttaa aagtcgagaa gccataggct ctggcggctt
     1621 caatttcccc atgcagaacc gaacggattg ccccagcaaa aatctcggta gtgtaagcgc
     1681 aggtgttaag cgtcagcgcc agcacggtac agttcaggcc actgcggaag aaagcgttaa
     1741 ggaattcggt tcccttaaca atctcaagcg tgtatatgcc ggaatagaac accagcaact
     1801 gaacatacag cggcgtaccg cgaaaaatat aggtaaataa ccagattgga aactggatgt
     1861 atttattact ggagacacga ccaatcgcca gaaacagcgc caggactccg cctatcacta
     1921 ccgacaaaat aagcagccac agagtgatcg ccacaccagt aaagcgataa ccgtcggtcc
     1981 acagcagcgg tttccagtat tcatgtaaga tttcgatcac aggtcagccc tcttcacacc
     2041 cacggagtag cggcgctcaa ggaacagcag cacaccattg gaaacagtgg tgaaaaccag
     2101 gtaaatcacg ccacagacga tggcgaaata gaacggttcc caggtacttt tgcctgccag
     2161 ttgggtggct ttgaccacat cttccaggcc gagtaacgaa accagtgcgg tagatttgag
     2221 gatcacctgc cagttgttgc caatgcctgg cagggcgtaa cgcatcatcg ccggaaacat
     2281 gatccgccga aacacttgcc cacgagtaaa accgaacgcc gtcgccgcct ctatatgtcc
     2341 tttcggcact gccataaaag caccacggaa cgtttcggta aaataagcac cgtagataaa
     2401 accgagagtg ataataccag cgaccatcgg atcgatatca atctgcccga cgcccatcgc
     2461 ctccgtcacc gtgtttagcg caatctgcaa accgaagaaa atcagcagca tcaacactaa
     2521 gtccggcacg ccacgaatca gcgtggtgta cccttcgaaa atcagccccg aaagccgatt
     2581 ttgcgagagc ttaccgccag cgccaattaa accgatgatt acagcgagca ctacagagct
     2641 gatagccagc tccagcgtga cgagcgcacc ctgtaaaata acacctgaaa acccatataa
     2701 catgctgcct gtcctgtcgt gtgtggtgaa ttaccgcctc ttgtctccct ccggttaccc
     2761 gaagggagcg ggctttcttt caccgatggc gattagccac cataaacatc aaaatcgaag
     2821 tactttttcg ctaatttctc gtaagtaccg tcagcgcgca tttcggcaaa ggctttgttc
     2881 agtgcttcgc gcagttcgtt atcttcttta cgcaggccca tgccggtccc tacgccaaac
     2941 agtttttcat ctttaacaga cgggccaccg aatttgtaat ctttaccgac aggttgtttg
     3001 aggaaacctt cgctggcagc gacctcatcc tggaacgcgg catcaatacg tccggcagtc
     3061 aggtcagaat aaatgttgtc ctgcccctga tacgagacga tttcaatgcc ttttggtgcc
     3121 caatgttcat taccgaacgt ctcctgggtg gtgccctgca atacgcctac ccgtttgcct
     3181 ttcagcgact cgactgtcgg ctgaatgtca gaatttttcg ccaccaccaa acgagaatcg
     3241 gcagcgtaca gtttgtcggt gaaggctatt tcttgctgac gtttttccgt aatggaaagc
     3301 gatgacatga tggcgtcaat cttcttcgct tttaaggacg ggattaacgc atccagcgga
     3361 ttttcgacaa acgtacattg cgtattgatg cgtttgcata attcctttgc cagatcgata
     3421 tcgaagccaa ccagttcgcc ttgtgaattt tttgattcaa atggcgcata ggtcgggtcg
     3481 gtaccgatgc ggatgttttg cggaatcgca gcaaatgccg cagttgcgct ggagaaggcc
     3541 agaaccagag agagcgatag caccagtttt ttcataatgt cctcaactga cagtcttttt
     3601 atagggaata tttacaggtt tgttacactt atcgtgccat aaaattgacc atcaaggcaa
     3661 aatatcccgc cccgttagat gtattatttt tcattaaaaa tgcttaagta tgcatttcaa
     3721 tgcaccacga tagtgcaaca aacctagcgt gcaccatgat gatcattcca tcaggtacag
     3781 cttcccagcg acgtatcagt caccgtagac attaaagtcg aaatactttt tcgccatctt
     3841 gtcgtaggtg ccgtcctgac gcagctcgcc aagcgcctta ttgaaggcag ccgtcagttc
     3901 agcatcatct ttacgtagcc ctacaccggt gccatcaccg aagtattttt tgtcttttac
     3961 tgatgagcca gcaaaggcga aatctttacc agcaggttgc ttgaggaatc cttcgctggc
     4021 agcaacttca tcttgtaacg cagcatccag acgtcctgca gccagatcgg aatagaccaa
     4081 atcctggttg gcataggcca ccacatccac gcctttgcta cgccaggtct cgttagcgta
     4141 agcttcctgg gttgatccct gcagcacacc aacatgttta cctttcagtg aatccagcgt
     4201 tggctgaatc ggtgaacctt tggcagcaat caaacgagaa tctgcggcgt acagcttgtc
     4261 ggagaaggca atctcctgct gacgtttatc ggtaatggaa agcgacgaaa taatggcgtc
     4321 gatttttttc gctttcagtg aggggatcag cgcgtcaaag tcactggcaa cccaggtaca
     4381 tttcacctgc atccgtttgc acatctcgtt accgagatcg atatcaaagc caacaaaatc
     4441 acctttagca tctttcgacg agaacggcgc ataggtggta tcggttccga tacgtaccgt
     4501 ctccggtagc gccgcatagc tggaagccgc tacggagaga ccgactaaca aagacagagc
     4561 gagaatcgac ttcttcatac ataaccctca agtgaaaatg gcttttttat gttgtgtatt
     4621 gtgttgtgtg tttgcaggct ctttcatgca ggtcttatgc catcttgacg acaacagtaa
     4681 cattcaacgt taaatatgtt aataagacgt tgcattattg tcctgaagtt gaagatagca
     4741 ggtatagcgg ttgaatcgca gcgtttcgat tgaatggcag aaacaaaatg tcgaggattt
     4801 gatcgcggtt gcaaaattgc cctgaaacag ggcaacagcg gagttatgcg ccctgccagc
     4861 gggcaaagag atcttcagga aggcttattg caaactggtc aagaacacga ttaaccgtct
     4921 gatttatcac atcatcaagg gattgcgggc gatgataaaa cgccggaaca ggaggcataa
     4981 tcaccgcacc gatttctgcc gcctgagtca ttaaacgcag atggcctaag tgcaatggtg
     5041 tttcacgcac gcagagcacc aacgggcgac gctctttcag caccacatct gccgcacggg
     5101 tcagtaagcc atcagtatag ctatggacaa tgccggaaag ggttttgatt gaacagggta
     5161 aaatcaccat ccccagcgtc tggaaagaac cggaagagat gctggcggaa atatcgcgcg
     5221 catcgtgcgt gacatcagct aatgcctgca cttcgcgcag ggaaaaatcc gtttcgaggg
     5281 ataaggtctg gcgcgccgcc tggctcatca ccagatgcgt ttcgatatct gtgacatcgc
     5341 gcagaacctg taataagcgc acgccataaa tcgcgccgct ggcaccgctg atgcctacaa
     5401 tgagtcgttt cataaatagt tgccctgtca gacttgcagg cagactttgc agtatttcgg
     5461 cgggagttgc aagtcagggt gccagaccgg caccctcagc gaaggcatca tccttcgtta
     5521 tgcatttcga gattttccac ttcgttctga cgttgcactg ctttggcgtc atcattacgt
     5581 aacgtatcga ggaaatcgag gtagtcctga tcaacatctt tggtgacgta gacgccgttg
     5641 aacaccgagc attcaaactg ctggatatcc ggattttcag cgcgaacggc gtcgatcaga
     5701 tcgttcagat cctggaaaat caacccgtca gcaccgatga tctggcgaat ttcatcaact
     5761 tcgcgaccgt gagcgatcag ttccgtggcg ctcggcatat caataccata aacgttcggg
     5821 aagcgaattt ccggggccgc agaagcgagg tacactttct tcgctccggc ttcgcgtgcc
     5881 atctcgataa tctgctcaga agtggtgcca cggacgatgg agtcgtcgac cagcaggacg
     5941 tttttatcgc ggaactcggc gcggttggcg ttcagtttac ggcgcacgga cttacgacgc
     6001 agctgctggc ccggcatgat gaaggtgcgg ccaacatagc ggtttttaac gaagccctgg
     6061 cggtacggct tgcccagaat acgagcaatt tccagcgcga tatcacacga ggtttccggg
     6121 atcgggatca ccacgtcgat atccagatct tcccattcgc gggcaatttt ctcacccagt
     6181 ttcgtgccca tattcacacg cgcgctgtaa acggaaattt tgtcgataaa cgagtccggg
     6241 cgggcaaagt atacatactc aaacaggcac ggattgctga ccggattgtc agcacattga
     6301 cgggtaaaca actgcccttc ttcagtgatg taaatcgctt cgcccggcgc gacgtcacgc
     6361 aggaaatcaa agcccagcgt atcgagcgct acgctttcgg aagcgaccat atattctgta
     6421 cggttctcgt caatatcacg ttttcccagt accagcggac gaatcccgtt tggatcgcgg
     6481 aaagcaacca taccgtggcc gataatcatc gccacacagg catatgcgcc gcggattaag
     6541 cggtttgtgg cagcaatggc agcgaaaata ttgtcggctt ccagcgggta gtggcggaag
     6601 ttgtccagct cgctagcgaa gatattaagc agaatttccg agtcggaagt ggtgttgatg
     6661 tggcggcgtt tttcttcaaa cagtttttta cgcaactcgt gagcgttggt cagattgccg
     6721 ttgtgggcaa gcgtaatgcc atacggggag ttaacgtaaa acggctgcgc ttcagaggcg
     6781 ctggagctgc cagccgtagg gtaacgcaca tgaccaatgc ccatattgcc ctgcaaacgc
     6841 tgcatatggc gagcttcaaa tacatcgctc accagcccgt tcgctttacg caaacggaag
     6901 cagttattgg catctatggt gatgatgccg gcggcatcct gaccgcgatg ctgaagcacc
     6961 gttaaggcat cataaatcga ctggttaacc ggcataacac cggcgatacc gacaataccg
     7021 cacatacgtc tttttcctcg ttaagccaca tctcagagca cttacgctct gggcaagaaa
     7081 cttgacgagc tttgcagata atcaaaaaac catctgatga taaaactgaa ctgcgggatc
     7141 agctgtgatt tgctccagtc ttcgcttttc gacaccccgg taaaggagtc gagaaagaag
     7201 agaatggcgg caacaatcaa cacaccgcgc aacgcaccga aacagacgcc cagcacccga
     7261 tcggtgcctg acaaccccgt tttctccacc aactggccta tcacgaagtt cacgatagca
     7321 ccaacgatca gggtagcgat aaacagtacc gcgatggcaa ttccatttcg aaccagttcg
     7381 tcttcaaagc ccgtaaacca gactgacagg taagtgtagt aatgactggc aacaaagaaa
     7441 gcacaacccc atgtcaccag cgataacgct tcacgaacaa agccgcggat caggctaacc
     7501 agagaggaaa aagcaatcac cgcgattatg gcgtaatcaa tccagaccat atgtgtccca
     7561 cgattttacg ccctgtcatc ctgttcgggg cgcattctaa cagaaaaaga aaacgtttgc
     7621 gtagggattt ccttcccacg catcaataaa aatggcgctg aaaaaatatt caacgccatc
     7681 gactttttat gcctttgcgg catcgtgcaa tgcgtgtcgg atgcggcgta aacgccttat
     7741 ccgacctacg gttctaacac tgcccaagcc tgataagacg cgccagcgtc gcatcaggca
     7801 agactgtatt aattcggcgt atagcccatt accacgccac ttaagccaga aagttgcttc
     7861 aactcaccca gcgaaccttt cagcttatct ttcgaggcat ccggcccaac cagaatacgg
     7921 gtaattttac cctgcactgg cgtggatggc gacgtataaa cccgataacc ggcaccgcgc
     7981 agcttaccga caatctcatt cactttatcg gcatttttca gcgcacccag ttgcacaaca
     8041 taggctttac ccgttggcgc tgctttttct tccacgaccg gcttcggttc tggcttcggc
     8101 gcaggtggtg cttcaacctt cggtttaggc ggctccaccg gtttcggctt cggtggggcg
     8161 accggtgcag gttccggttc aaactcggtg ttattggctg caatagtggc cggatcgagc
     8221 gacggtgctg cggcatcacc tgcccgcacc tcttccgctg cgccttccgg cggctgcgtc
     8281 ggcaacgcct gggtggcggc tggcatcata tcaggctcat cgcgatcgcc cgcttttgga
     8341 accagcggga tagccgcaaa ctcatcctga taatgttttt tctgcccgtc cagcagccct
     8401 ggaagtacaa tcacccccag cgccaccagc acgatcgtgc ccactaaccg attctgaaac
     8461 ttacttgcca ccgcttctcc tcgcgtcaat cacttccatg acatgtgcga ccgtgtggaa
     8521 agaaccacac accagcacgg tgtcttccgc tttagcgtcc gccattgcgg catcccatgc
     8581 ctgcgcaacg ctatcaaatg atttgccgtt acccaaatgc tcaagcagtt gttctgccgt
     8641 ggcaccgcgc ggcccttcca gtggcgcaca ataccagtca tcaaccacgc ttttcaacca
     8701 ggccagagtt ccggcaatat ctttatcatg tagcataccg ataaccgcca gcacgcgccc
     8761 gtttttcggt agcgctttca tacgcccggt gagatattcc gccgcatgtg gattatgcgc
     8821 gacatcaaaa ataacgcgtg gcgactcgct cacaatctgg aaacgtcccg gcaaaattgc
     8881 gctggcaatc ccgtcgcgaa tggcattttc actgacttcc agcccgctgg cacgcagtgc
     8941 cgccagcgct gttgcggcat tcggttgcgg gacaagcggc aacggcagat tttccagcgt
     9001 gccgtgagca tcgctaaacg cccagtcatg atcggtgacg gaatagttcc actcaacgcc
     9061 ccgacgttgt aacagtgcac ctttttcctg cgccacatca gcaatggtag aaggcatttc
     9121 cggctcaccg acaattgccg gttttgcgct gcgaaagatg cctgctttct cgcgaccaat
     9181 actttcgcga tctggcccca gccagtcggt atgatccagc gcaatactgg ttactaccgc
     9241 gacatcggca tcgacaatat tggttgcgtc cagacgaccg cccagcccta cttccagaat
     9301 caccacgtca agttgtgcct gtttgaacag ccacaacgcc gacagcgtac cgtactcgaa
     9361 ataggtcagg gaaatatcac cgcgtgccga ttcaatctcc gcaaaagagg cggtgtgggc
     9421 cgattccggc aattcctgac cctgcacacg tacgcgctcg gtataacgca ccagatgcgg
     9481 cgaactgtag acgcccactt tgtaccctgc cgccatcaga atcgactcca gcgtacggca
     9541 ggtggtgcct ttgccattcg tacccgcaac ggtaaacaca aatggcgctg gtttcagaac
     9601 gccaagacga gccgcgacct ggctcacgcg ctcaaggccg agatcgatag ttttactgtg
     9661 caggttttcc agataagaaa gccacgaagc cagaggcgac gcggcttgag gagtgcgttt
     9721 gataatcatg gtatccgctg attcgttacg gtgagaatag caaaagggca gagccagtgg
     9781 ccctgccctt atcagttatc aggcctcagg ttcctgatcc ggtaccgggg gtaccactac
     9841 gccttcacgc ggcgcttcag gattcggcgc tggcagattc atcaacttcg ccagaatgct
     9901 cgccagtttc aggcgcattt ccggacgacg gacgatcatg tcgatcgagc ctttctcgat
     9961 caggaattca ctgcgctgga atcctagcgg cagtttttcg cgaacggtct gttcgataac
    10021 acgcggaccg gcaaagccaa ttaacgcttt cggttcagcg atgttgagat cgcccagcat
    10081 ggcgaaactt gcagaaacac cgcccatcgt cgggtcggtc agcacggaga tgtacggcaa
    10141 gccgcgctcc tgcatttttg ccagtgccgc agaggttttc gccatctgca tcagcgacat
    10201 cagtgcttcc tgcatacgtg cgccaccaga ggcggagaag cagatcagcg ggcagttatc
    10261 ttccagcgcc tgctcaacgg cacgcacgaa acgtgcaccc acaacagacc ccattgaacc
    10321 gcccataaag gcgaactcga atgccgcagc gacaaccggc attccataca gagtgccttt
    10381 catcaccacc agcgcatctt tttcgccggt ttctttctgc gcagaagcca gacggtcttt
    10441 atacttcttg gagtcacgaa acttcagcac atctttcggc tcaagctcgc tacccatctc
    10501 cacaaggctt ccttcatcta acaggctatg caggcgatta cgcgctgtca tacgcatgtg
    10561 atggtcacac ttcggacaga cctcaagatt acgttccagc tcagcgcggt ataaaacctg
    10621 accgcagcta tcacacttag tccacacccc ttcaggaatg ctcgccttgc gggtgggagt
    10681 aatgttgctt ttaattcgtt caatccagct cattagggac ctttctgtct gaacctggtt
    10741 cgatgccagt tttatctttg gggacgcata atgccatttt tgcccccaac agaccatgaa
    10801 tgttgcacat taaaacataa cagcccgaaa ctttggataa aaaagtggtc gaaccgcgga
    10861 gttacttttt attttgcggc gcgtgccgca gcgcgtttgt gacgaataaa ttcgaaaacc
    10921 ccaggcaata ttgaaacaaa aataatgcca acaatcatca gcttaaggtt gctctgaatg
    10981 aaggggagca tgccgaagaa ataacccgcg taggtaaaaa tcagtaccca caacagtgcg
    11041 ccgatcacgt tataagcggc gaaatgacgg tacgacatgt ggcccattcc cgcaacaaac
    11101 ggcgcgaacg ttctgacgat cggtacaaaa cgggcgagaa taatcgtttt gccgccatgt
    11161 ctctcgtaaa actgatgagt cttatcaaga taactgcaac gaaagatttt cgaatccggg
    11221 ttactgaaca gtttgtcacc aaataaccgt ccaatggtat agttcactgc atcaccgaca
    11281 atagcggcaa tcaacataag caccaccatc atatggacat tgagatcgtt agtttccagc
    11341 gacgccaatg caccagcaac aaacagcagc gaatcaccag gcaggaatgg cgttaccacc
    11401 agccccgttt cgcagaacag aatcaaaaac agaatggcat aaacccagac gccgtactcc
    11461 gcgaccagct ctgccagatg cacgtcaatg tgcaggataa aatcgatgag gaaataaatc
    11521 aggtccatat ttgcctatgc cttgtactcg tcatctttca ggctgtaact acgttggcta
    11581 cagcctgaaa tgttccgagc attattctcg ttagtccgcc agaaatagcg ggcccattgg
    11641 cggttttgga agatcatacc ggtcagggta atccaccgcg accagataca gcccttccgc
    11701 ttttgccgtt gctgccgcca gcgttctgtc ctttgccgcc agcaactctg ctatccagct
    11761 ctccggctgg ttgtgggcac cgacttccat caggctgccg acaatattcc tgaccatatg
    11821 atgtacaaag gcattcgctt tgatatctac caccacataa ggaccgtgac gcgtgacatt
    11881 aatatgcata acgttgcgcc acggggttcg ggactggcac tgcaccgcac ggaacgaggt
    11941 gaaatcattc tcgcccagca agcattgcgc agcccgatgc atccgttcag cgtccagcgg
    12001 ttcgtaaaaa tgggttaccc ctttactcag taccgccggg cgcagccgat gattgtagat
    12061 gatgtagcga taacggcgag ccgtggcgct aaatcgggca tgaaaatcat caggtacagc
    12121 tttaacccaa cgcacggcga tgtcatcagg taaattcgca tttacgccca aggtccacgc
    12181 cgcgtctttg cgctgcgcgg ttgtttcgaa atgcaccacc tgcccggtac cgtgtacccc
    12241 tgcgtcagta cgcccggcgc agaagacggt gatgggttcg ttcgccacct gggagagcgc
    12301 cttttccagc ttctcctgca cactgcggac ttcattctgc cgttgccagc cgtaatactt
    12361 actgccgtcg tactcaatgc ccagcgcaat tttataaact ggcagttgtt gctggtcgga
    12421 cattagtaca gatactcctg caccagtttc tcggcgattt ttactgccat cagcgcgccg
    12481 ccaaagcgaa cgttatcggc caccgaccag aactggactt gctccggcat accgtagtca
    12541 ttacgcacgc agccaacaga aagatgcgga gtacccgaag catcccctac ctgagtcggg
    12601 aattcgttct cttcagagag cacaatatct tcgccctgag caaacgcatc acgcgcttct
    12661 tctgccgcca gcggacgcag tgcttcaaag ttgaccatct gggcatgacc gtagaatacc
    12721 ggtgcctgga cgacgcttgc cgaaatcatc agcccttcgt cctgcaggat tttgcgtact
    12781 tcgtcaacga tacgacgttc ttcacgcacg ctaccttcgc tatccggcaa caacggcagc
    12841 atattgaacg ccagttgacg accgaagaaa tcttcttcgt caatcggaat gccgttgagc
    12901 aatttcgcac tctgccccgc taacgcatcg accgcttttt tgccctgggc ggaggctgaa
    12961 atcaggctgg taacactgat acgcgacaaa ccgccctgat cgattaacgg tttcagtgcc
    13021 gccagcagct ggctggtcag actgtctggt acggcgatga cattccggtt gcggtaatcg
    13081 gtcagtacaa acgggtttac ttccggcacc accagcggta cgtcgggttc gagagcaaac
    13141 agtccgctgc tgtcgatcac caggcaacct gagttggtcg cttcttcaac ccaggcagcg
    13201 gtagcttctt tgcctgcgac aaaaaatgcc agctgcgcct gcgtccagtc gaattcagcg
    13261 gcatcctgca cggtgattgt tttaccacca aagcgcagtt gttcgcctgc gctttcgtta
    13321 cgtgccagtg cataaatttc cccaaccggg aactgacgtt cagccagcgt ttcaagcagg
    13381 gcttcgccca cagcgccagt tgcgcccagg acggcaatgt tccagccttc agacatggtg
    13441 gtttactcca gaaatagcaa agctccctgc caatgttaca gcagagagca tgaagaagag
    13501 attaacgtgc cggatgatga acggcgttaa aacccagttt acacagcaat gatgccgcac
    13561 tggcgtcatc acaaattaca tacagagatg accattcacg gcgctcaaga tagtttttgc
    13621 gcagtttatc gaactcaccc ggtatcccgg cgactttacg cagcggtgcg tcatcgcggc
    13681 gcacatcata caccaaatgc accagccttt tcagcgtcgg ttgatcgagc gggccatgca
    13741 gcgtaatgcg accaaactct ggcgcgggca gtaatgtatc cagcgcaacg tgctgttcat
    13801 gcccaataaa cttgctataa gcttcaaaca cttgcgtagt accgcgtgct ttaccttcca
    13861 gggtataacc tgcgatatgc ggcgtgccga tatccacttt tgtcagcaac tccacgttaa
    13921 gttccggttc gccttcccag acatccagca ctacgcttaa cttctggcct tcattcaggc
    13981 aggtcagcaa cgcagtatta tcgacgactg cgccacggca ggcgttaatc agaatcgctc
    14041 ccggcttcag gctacggatc agtttttcat ccgccagatg cagcgttttg tacggcccat
    14101 ctttgaagag tggcgtatgg aaagtcagaa tatccgcatg ctgaactaat tcatccagcg
    14161 agcggaaatc accctcatcc ccacggtcag cgcgaggcgg atcgcaaagc aatgttttaa
    14221 tccctaacgc ttccagtcgc gcctgcaatc gacgtccaac gttaccaacg cccacgatcc
    14281 ccaccgtacg gtcgtgcagt gaaaatccat cgcgttcggc aagcatcagt agtgaggaga
    14341 aaacatattc caccaccgca atcgcattac agccaggtgc agcggaaaaa ccaattcccg
    14401 cctgcttcaa ccatgcttca tcgacatggt cggtccccgc agtggcagtg ccaacaaatt
    14461 taatgggttt tcctgccagc aaagattcat tcactttcgt gaccgaacgc accatcagcg
    14521 catccgcgtc tgccagttga gcgacggcga ttggacgccc gggaaccgcg gtcacctcac
    14581 ccaaacggct aaataagtcg cgggcataag gcatattttc atcaacaagg attttcacgt
    14641 ttgtgttacc tgtatgagac gagagttaac cggacaagtg tgccataatc tcgcggccag
    14701 gcatacttgc gaagatttca ggtataagga tacgtaatga tacaacctat ttccggccct
    14761 cctcctgggc aaccaccagg tcagggagat aacctgccgt ctggcgcggg caatcagcct
    14821 ttatccagtc agcaacgtac ttcgctggaa agcttaatga cgaaagtgac ctcactgacg
    14881 caacagcaaa gagcagaact gtgggcgggt atcaggcacg atattggtct gtcgggagat
    14941 tcaccgctgc tttcgcgtca cttccctgcc gctgagcata atctggcgca acgtctgctg
    15001 gccgcgcaaa aaagccattc tgcccgccag cttttagcgc aattagggga gtatttacgt
    15061 ctggggaata atcgtcaggc ggtcacggat tatatccgtc ataactttgg tcagacgccg
    15121 ctgaatcagc tctcaccgga gcaattaaaa accattctca ccctgttgca ggaagggaag
    15181 atggttattc cgcaaccgca gcagcgcgag gcgaccgacc gtcctttatt accggcggag
    15241 cacaatgcgc taaaacagct ggtgaccaaa cttgcggcgg caacggggga acccagcaaa
    15301 cagatctggc aatcgatgct ggaactttcc ggggtgaaag atggcgagtt aattccagcg
    15361 aaactgttta accatctggt gacctggcta caggcgcgtc agacgctaag ccagcaaaat
    15421 acgccgacgc tggaatcact acagatggcg ctaaaacaac ctttagatgc cagtgaactg
    15481 gcggcgttat cggcatatat ccagcaaaaa tatggccttt ctgcgcaatc atcgctttct
    15541 tctgcccagg ccgaggatat tcttaatcag ctttatcaac ggcgggttaa agggattgat
    15601 ccgcgtgata tgcaaccgct gcttaatcct tttccaccga tgatggacac gttgcaaaat
    15661 atggcaacgc gtcccgcgct gtggatactg ttagtcgcga ttatcctgat gctggtctgg
    15721 ctggttcgtt aaccccgacg aaatgacagt atcgtgacaa taatacccag caccgcagag
    15781 atcgccccgg caagaaatac cgaagagtaa ccaaacgtgg tcgccagcat tcccgccagc
    15841 ggcccggaga cgccaagggc gatatcctga aacgcggcgt aaccgcccag tgcggtgccg
    15901 cgaacttgtg aggggacgcg tttaaccacc tccacgccca gcgcaggaaa gataagcgaa
    15961 catccggctc cggttaacgc cgcgcccgct aatgcgaccc atgcacctgg ggcttgccag
    16021 agcagcaaca agcccaccgt ttctacaagc agagagacaa tcgccacttt cacgccgcca
    16081 aaacggtccg gcatccagcc aaacatgacg cgcatcacga caaatgcgcc gccaaacgcg
    16141 gtaagagtaa agcccgccat cgcccatcct ttgctggcaa agtagagcga aacgaaagtc
    16201 ccgataaccg caaaaccaac gccttgtagt gccagcccta accctggttt ccagataagc
    16261 ccgacaacgc tccacagcga tggacgttct cccgccaggg ccggtacttt gcgcactgtg
    16321 ccgttacagg cccacgccag taagggtaat accattgtgg tgatcgccag tgcggcaaaa
    16381 ccgtaatggc tatgaatcaa caggccaagc ggcgcaccaa cagcgagggc accgtaaatc
    16441 gccattccgt tccatgacat cactttgcca gagtgttttg cccctacgat gcctaatccc
    16501 caggtcagag cgcctgtcag taactggctt tcaccaaagc caagaatcaa acgcccgacg
    16561 accaacaggg caaatttcaa cggtgcggag acaggcaaaa tcgccgccag cagcaacgcg
    16621 ccgccagcca gaccacaagc taacatcccc tgaagcgccg aacgttttgc accatattga
    16681 tcggccagtc gcccggcata gccacgcgtc agcaccgtag ccagaaactg aatcccgacg
    16741 gcaatcccga ccatggtatt gccatagccc agatcatgat gaacaaacag cgggataacc
    16801 ggcaacggca gccctacggt catgtaggtg agaaaaaccg caaaagcgat gcggaagagc
    16861 gaaaaattgg cagaagatcg tgtttcggtt tggcttacag cagtcatgca ttactccaga
    16921 atgcagcgca aggcgaggag tatccccgtc tcatctctct ggtttcaggg ttacagtgcg
    16981 ttggcaggat ttaacgcgta cgtcttttca gaaggaaatc gacaaagcgg gaagtttgcc
    17041 tggaactggc ggcgattgtc aatgatgtga aaaagggaac catcaggttc ccttttgcgt
    17101 tagtgccgga ggccgcattg gcgcgtaacg tcggatgcga cgctggcgcg tcttatccga
    17161 cctactccga ctactccgac tactccgact actccgacta ctccgactac tccgacctac
    17221 tgcgaattaa tctttcagct tgcgcattac cagcgtggcg ttggtgccgc cgaagccgaa
    17281 gctgttagac ataacggtgg tcagttcgcg atcggtcgtt tcggtcacga tgttcagacc
    17341 tgccgcctgc tcgtccagct cttcaatgtt gatgctcggg gcgataaagc cgtgttccag
    17401 catcagcaga gagtagatag cttcctgtac gccagcagcg cccagagagt gaccggtcat
    17461 ggctttggtt gcagaaatcg ccgggctctt atcgccgaac acttcacgga tagctgccag
    17521 ttctttcacg tcgccaaccg gagtcgaagt accatgagag ttcaggtaat cgattggggt
    17581 atcaacgccg tgcatcgcca tcttcatgca gcgtactgcg ccttcgccag acggagcaac
    17641 catgtctgca ccatcagagg ttgcgccgta gccaacgatt tcagcataga tgtgagcacc
    17701 acgcgccagc gcgtgttcca gctcttcaac cactaccata ccgccgccgc cagcgataac
    17761 gaaaccgtca cggtgagcgt cgtaagtacg ggaggctttt tccggggtgt cgttgtattt
    17821 agtagacagc gcacccatcg cgtcgaattc gcaggccatt tcccagcaca gctcttcgcc
    17881 gccgccagca aacacgatgt cctgtttgcc cagttggatc tgctctactg cgttaccgat
    17941 acagtgtgcg gaagtcgcac acgcggagct gatggagtag ttaacgccat gaattttaaa
    18001 cggcgtggcg aggcaggcag aaacgccgga tgccatcgct ttggtgacca catacgggcc
    18061 aaccgctttc aggccgcgcg ggccgcgcat tgcgtcagcg ccgaatacct ggaaacgcgg
    18121 ggaaccgccg ccagaacctg caatcaggcc aacgcgcggg ttattctggt aagcttccgg
    18181 agaaaggccc gcatctgcga ttgcctgctc catagaaagg aatgcataaa tggatgcgtc
    18241 gctcataaag cgcacaactt tgcggtcaat gaggccagtg gtatccagtt ttacgttgcc
    18301 ccagacgtgg ctacgcatgc cggaatcctt aagctcctga gagaaagtga tccctgaacg
    18361 tccttcacgc agagatgcca ggacttcctg ctggttatta ccgatgctgg aaacaatgcc
    18421 caggccagta atcactgcac gtttcattca atacctctgt aagtcgcaca tagagtaagt
    18481 ttcgaatgca caatagcgta cacttgtacg ccgaacaagt ccgatcagcc atttaataga
    18541 gaaatttgcg cagccttaca cacatcgcta agatcgagcc accgcctgta agacgagtaa
    18601 cttacgtgaa acactactcc atacaacctg ccaacctcga atttaatgct gagggtacac
    18661 ctgtttcccg agattttgac gatgtctatt tttccaacga taacgggctg gaagagacgc
    18721 gttatgtttt tctgggaggc aaccaattag aggtacgctt tcctgagcat ccacatcctc
    18781 tgtttgtggt agcagagagc ggcttcggca ccggattaaa cttcctgacg ctatggcagt
    18841 catttgatca gtttcgcgaa gcgcatccgc aagcgcaatt acaacgctta catttcatta
    18901 gttttgagaa atttcccctc acccgtgcgg atttagcctt agcgcatcaa cactggccgg
    18961 aactggctcc gtgggcagaa caacttcagg cgcagtggcc aatgcccttg cccggttgcc
    19021 atcgtttatt gctcgatgaa ggccgcgtga cgctggattt atggtttggc gatattaacg
    19081 aactgaccag ccaacaggac gattcgctaa atcaaaaagt agatgcctgg tttctggacg
    19141 gctttgcgcc agcgaaaaac ccggatatgt ggacgcaaaa tctgtttaac gccatggcaa
    19201 ggttggcgcg tccgggcggc acgctggcga catttacgtc tgccggtttt gtccgccgcg
    19261 gtttgcagga cgccggattc acgatgcaaa aacgtaaggg ctttgggcgc aaacgggaaa
    19321 tgctttgcgg ggtgatggaa cagacattac cgctcccctg ctccgcgccg tggtttaacc
    19381 gcacgggcag cagcaaacgg gaagcggcga ttatcggcgg tggtattgcc tgcgcgttgt
    19441 tgtcgctggc gctattacgg cgcggctggc aggtaacgct ttattgcgcg gatgaggccc
    19501 ccgcactggg tgcttccggc aatcgccagg gggcgctgta tccgttatta agcaaacacg
    19561 atgaggcgct aaaccgcttt ttctctaatg cgtttacttt tgctcgtcgg ttttacgacc
    19621 aattacccgt taaatttgat catgactggt gcggcgtcac gcagttaggc tgggatgaga
    19681 aaagccagca taaaatcgca cagatgttgt caatggattt acccgcagaa ctggctgtag
    19741 ccgttgaggc aaatgcggtt gaacaaatta cgggcgttgc gacaaattgc agcggcatta
    19801 cttatccgca aggtggttgg ctgtgcccag cagaactgac ccgtaatgtg ctggaactgg
    19861 cgcaacagca gggtttgcag atttattatc aatatcagtt acagaattta tcccgtaagg
    19921 atgactgttg gttgttgaat tttgcaggag atcagcaagc aacacacagc gtagtggtac
    19981 tggcgaacgg gcatcaaatc agccgattca gccaaacgtc gactctcccg gtgtattcgg
    20041 ttgccgggca ggtcagccat attccgacaa cgccggaatt ggcagagctg aagcaggtgc
    20101 tgtgctatga cggttatctc acgccacaaa atccggcgaa tcaacatcat tgtattggtg
    20161 ccagttatca tcgcggcagc gaagatacgg cgtacagtga ggacgatcag cagcagaatc
    20221 gccagcggtt gattgattgt ttcccgcagg cacagtgggc aaaagaggtt gatgtcagtg
    20281 ataaagaggc gcgctgcggt gtgcgttgtg ccacccgcga tcatctgcca atggtaggca
    20341 atgttcccga ttatgaggca acactcgtgg aatatgcgtc gttggcggag cagaaagatg
    20401 aggcggtaag cgcgccggtt tttgacgatc tctttatgtt tgcggcttta ggttctcgcg
    20461 gtttgtgttc tgccccgctg tgtgccgaga ttctggcggc gcagatgagc gacgaaccga
    20521 ttccgatgga tgccagtacg ctggcggcgt taaacccgaa tcggttatgg gtgcggaaat
    20581 tgttgaaggg taaagcggtt aaggcggggt aatctgctct cgcagtgttt gccggatgcg
    20641 gcgtgaacgc cttatccgac ctacgtgtga cgtgtaggcc tgataagacg cgataagcgt
    20701 cgcatcaggc atggtgctcc agacgccgca acttactgct gtgacgcctg ctgaaacagg
    20761 ttttcccaca tatcggtcac cagcgcctgg tcacgcggcg acaattcacc ggcaccaatg
    20821 gctttttcca gactctggct aacggtcgta tgcaccgcct gagcggagtg gtcatcacca
    20881 ctttccagtt ctgcgatggc taatgtcagg tggccacgca aatacccact ggcaaacaac
    20941 tcatcatcac tggcatggtc aatcatgccg tcgattaatg ccagaatgcg tgattcaaac
    21001 tccgcgatca tcttctttcc tcattgtaaa acgtggcacc ggcttcagtt gagcgcttcc
    21061 ggccacggga actgttccgc cgtaagttcc ggcgtgtgat aataattttg tagtgcttta
    21121 atgaagcgtg ccggacgctc gggaatgcca tttgtcagat aatccatcac ctgcgcatgt
    21181 acccgccgtt gaaacaccac acgatccggt tcgaagtcgc cttccagatt gtcgcaactg
    21241 acgttaaacg gatatcccgc cgccacacag aacaaccagt cgagtgcctg cggcttcact
    21301 tcaacatctt caaactggct ttgcgtttgg gcatcacgcc catccgggca ataccagtag
    21361 ccgaaatcaa ccagctcacg gcgcgctttt ccggcaatac accagtgcga aatctcatga
    21421 attgcgctgg cataaaagcc gtgggcaaag acaattcggt tatacggtac ttccgcatca
    21481 gcaggaagat agatcggttc gtcgtcgcct ttaatcagac gggtattaaa ttcatcggca
    21541 aagcagctat taaaaatttc aatcaactgc tcatagtggt gtgtactgtt cattagttca
    21601 tccccaacca gtggaggatc tcctgtccgt ggctatcgta aagtaatttg gcactcatca
    21661 ccgccgagac gataacgatc atcgggcgga tcagtttttg tcctttgctc agcaccagtc
    21721 ttgaacccat gcgcgcgccg aggaactgcc ccaccagcat gacaaagccc gtcgcccaaa
    21781 tcactttgcc gccgagaata aacagcagca gaccgccaat gtttgacgtt gcgttgagta
    21841 atttggcgtg agccgtggct ttggcgaggt tgaatccgca cagcgtaacg aaggccagcg
    21901 cgtaaaacga tccggcagcc gggccaaaga atccgtcata aaaaccgacg cagccaccgg
    21961 caattaacgc gaagggtaaa ccgtacatcc ggcgctggcg atcttcttca cccagctttg
    22021 gcatcagcaa gaaatagagg ccgatacaaa ttaccagaat gggcaaaatc tgccgtaaga
    22081 catcagcctg aacgtattgc accagcagtg cgccgctcat tgagccggca aaggtcatgg
    22141 cgatattgag tttctgatcg cttaaactaa ccactttgcg gcgaataaag tagatagtgg
    22201 cggaaataga gccgccgcag gcttgcagtt tattggttgc cagcgcgtta gcgggagaca
    22261 ttcccgcagc catcaatgcc ggaatggtga gtaacccacc gccaccggca atcgagtcga
    22321 taaatcccgc cagcatggcg acaaaaaaga gaactcccag caacagcggg gaaaccataa
    22381 acaggctatt aaacgtttcc attagatcac gtgctcatcc agtagcgcct ggcaggaagg
    22441 cggcaacgga ggcggtgtct tcttctcagg ctttgttgtt cctggttttg gaggtgcaaa
    22501 ccagctttgc agttctgccc cgcaaccatc gcctggtggc ggtaaaggtt gatcttcaca
    22561 ctccagacta tcggcagggc aacgtaatcg tacatgcata tgcgcgcgat gctggaacca
    22621 gggtcgcact ttgcgcaacc agtcgcgatc ggtgcccgca tcaaggcaaa gttgttgttt
    22681 aatcgccgga ttaacaaaaa tgcgcgtgac gtctttgtcc tgggcggcga gtttgatcaa
    22741 gctgaaaatt tctggcttcc acagcgtgga gacaacgtgt ttaccgtcgc gggaaactaa
    22801 gtccagtgct tgcgggcgca agagctgcgc ggaggtccag cgagttttcg gcagttgcag
    22861 aaagatatcg acatccagtc cggtctggtg gctggcatga ccgccgttga aacgcccacc
    22921 agcgggcatc cccatatcgc caatcagcac cgtacccatg cccagattgc tcacctggct
    22981 actcagacgc tggataaaca tcaccagatc cgggtgaccg aaatagcgac gctgatcggt
    23041 acgcatgacc tgataatgtt cggactgtat cggcaacgtg tcagcgccga caatacagcc
    23101 attagaaaaa ctgcctatcg attgtgcgct acccggcaca ggttgggtta ttttttgcca
    23161 cggcgtcgct gccaagctag cgctactggc aagcagagcc agcagcgcaa tcgcggtttt
    23221 gttcattttt taccagcgtg gaatatcagt cttcacatcg gcattttgcg cccgctgccg
    23281 taacaggtga tccattaaaa cgatcgccag catcgcttca gcgatcggca ctgcacggat
    23341 cccgacacag ggatcgtgac gacctttggt gatcatctca acttcttcgc caaagcggtt
    23401 aatagtgcgc cccggcacgg taatgctgga ggtcggtttc agcgccatat gagcaatgat
    23461 ttgctgcccg ctgctgatac cgccgagaat gccgcccgca tggttgctct ggaaaccgtc
    23521 tttggtgatt tcgtcgcggt tctggctgcc gcgcagcgcc actacgtcaa agccgtcgcc
    23581 aatttccacg cctttcaccg cgttgatgct catcagcgca tgggcgatgt cggcatccag
    23641 gcggtcaaat accggctcgc caagtccggc ggggacgcca ctggcaacaa cggtgacttt
    23701 cgcgccgatg gagtcgccct cttttttcag cgcgcgcatc agttcatcta acgcgtcgat
    23761 tttgtccgga tccgggcaga agaacggatt ttgttcaacc agcgaccagt ctttgatttc
    23821 cagcggaatg tcgcccatct gggtcaagca gccgcggatt tcaataccaa atttctcggc
    23881 gagatatttt ttggcaatcg cccctgccgc cacgcgcatg gcggtttcgc gggcggaaga
    23941 acgtccaccg ccgcgataat cgcgcagacc gtatttttgt tcgtaggtgt aatcagcatg
    24001 gcctgggcgg aaaacgtctt taattgcgct gtaatcctga gagcgctggt cagtgttttc
    24061 gatcaacaag ccaatgctgg tgccagtagt aacgccttca aaaacaccag agagaatttt
    24121 gacctgatcc ggctcgcggc gctgggtggt atagcgcgat gtcccagggc gacgacggtc
    24181 gaggtcatgt tgcaggtccg cttccgtcag cagaatgcct ggcggaacac catcgacgat
    24241 gcagccgagc gccagcccat gcgattcgcc gaaggtggtt acgcgaaaga gttgtccaat
    24301 tgtgtttcca gccatcacgg ctccgttatt gttgtgtttg cgtgtttact taatctttat
    24361 aaatcgcgaa atattcacgg gccgccagca gctgctcttt ggtgagcata aacacaccat
    24421 cgccgccgtt atcaaactcc agccaggtga acggaacatc cggatattgt tccataagat
    24481 gtaccatgct gttgccgact tcacaaatca acacgccatc atcagcaagg taatctgccg
    24541 cgtttccaag aatgcgacgc gtcagtttaa ggccgtcggt gccagatgcc aggcccagtt
    24601 ccggctcgtg acggtattcg tttggcaggt cggacatatc ttccgcgtcg acatacggcg
    24661 ggttagtgac aatcagatcg tactgcactt tcggcaagtc gcggaacaga tcggaacgaa
    24721 tcggaatgac gttgtggatc agaccgtgtt cttcgatgtt ctgttcagca accgccagcg
    24781 cgtcgggaga gatatccacc gcgtcgactt ctgcatccgg gaaggcataa gcacaggcaa
    24841 tggcgatgca gccgctaccg gtacacatat ctaaaatatg ctgcggttgt ttgctgatga
    24901 gtccggcaaa tttattgttg atcagttcac caatcggcga gcgcggcacc agcacgcgtt
    24961 catcgacgta aaattcatga ccgcagaacc aggctttgtt ggtcaggtaa gccaccggaa
    25021 tgcgctcgtt gacgcggcgg atcacgcgtt caacaatacg atgtttttcg ctggaggtca
    25081 gacgcgcggt gcgcatatct tccgggatat ccagcggcag gtagagcgat ggtaacacca
    25141 gctgtacggc ttcatcccac gggttatcgg tgccgtgacc gtaccagata tttgccgcgc
    25201 tgaagcggct cactgaccag cgcaacatgt cctgaatggt ttgcagctca tttactgctt
    25261 catcaacgaa aattttatcc acgtattcct ccagggcatg atcgcaataa tttcggcggc
    25321 tagtttgcca tgaagatgac gataaatcag cattcacgcg cggtgagtga ggaaaaatac
    25381 gtttaaaacg atcgattgtg ctacgagtcg ggtaaactgt aggaaaatta gaaatagaga
    25441 cagataaatg aaaaagaaaa caacacttag cgaggaggac caggctctgt ttcgccagtt
    25501 gatggcgggg actcgcaaga ttaagcagga cacgattgtc caccgaccgc agcgtaaaaa
    25561 aatcagcgaa gtgccggtga aacgcttgat ccaggagcag gctgatgcca gccattattt
    25621 ctccgatgag tttcagccgt tattaaatac cgaaggtccg gtgaaatatg ttcgcccgga
    25681 tgtcagccat ttcgaggcga agaaactgcg ccgcggcgat tattcaccgg aattgttttt
    25741 ggatttacac ggtctgacgc agctgcaggc caagcaggaa ctgggcgcgt tgattgccgc
    25801 ctgtcgccgc gaacatgtgt tttgtgcctg cgtgatgcat gggcacggga agcatatttt
    25861 gaagcaacaa acaccgctgt ggctggcaca acatccgcat gtgatggcat ttcaccaggc
    25921 accgaaagag tacggcggtg atgcggcttt gttggtgttg attgaagtgg aagagtggtt
    25981 gccgccggag ttgccctgag gagttgagcg tgttgtgcca ggagcgcgga atggcagggg
    26041 cgaatgaccc tgccaggtga gaaaaggtgc gctaatacat attcagtatg taagtgaatc
    26101 ggcaggtaga atctatgggc tgtcgagatg tcgtgtccac gcgtagcttc atatcatgca
    26161 gttgcctgga cgtaatcggt tgcggctcag gcgtcaccgt cactttggca ttatcacagc
    26221 cggaagtcga gaaggtcatc tgctgtgcga taaaacggca gtagacacca cgctcatcaa
    26281 aattatttcc gttacacagt ttgccatttt ccatcaggaa agtgggcagt gtttgctcaa
    26341 taacaccacc tgttgactgc aacctgacga ctttgttatg tacaacataa ttttcattcc
    26401 aggaaacatc atacggtgcc aggggcaccg ccggacatga agaactcacc ttcgttgggc
    26461 caatatagtc aggaaaatca tcatcatacg ttttagcgaa gggatctcta ctcttaacaa
    26521 acgacgacac aatccacgga caaccatgta aattcccatc gtaataaaca aacatcttta
    26581 attcattaca tgtccatccc gttttggtct ggctatcata ctgctctaat ccaggacagg
    26641 ctgacatgga taccctgctg tccgaaattt tcgacataga tggttgagta gaactatcgc
    26701 cagtccacga aactaaatcc ggctgtgaat tgggcatgaa tgtagcctta aatatagcgt
    26761 aattaactga atctggcgtc gctataatca gatgaaactc aaactc
//
LOCUS       DAJTMC010000042        25509 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.042, whole genome shotgun sequence.
ACCESSION   DAJTMC010000042 DAJTMC010000000
VERSION     DAJTMC010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25509
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.042"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            <1..183
                     /locus_tag="OO130_001557"
     CDS             <1..183
                     /locus_tag="OO130_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HCR5365015.1"
                     /translation="ACMAITNLTAILLLSPVVHTIASDYLRQRKLGVRPVFDPLRYPD
                     IGRQLSPDAWDDVSQE"
     gene            253..1029
                     /gene="yaaA"
                     /locus_tag="OO130_001558"
     CDS             253..1029
                     /gene="yaaA"
                     /locus_tag="OO130_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HCR5365016.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPANARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(1182..1478)
                     /locus_tag="OO130_001559"
     CDS             complement(1182..1478)
                     /locus_tag="OO130_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCR5365017.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGNRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(1692..2978)
                     /gene="thrC"
                     /locus_tag="OO130_001560"
     CDS             complement(1692..2978)
                     /gene="thrC"
                     /locus_tag="OO130_001560"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HCR5365018.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLCLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(2979..3911)
                     /gene="thrB"
                     /locus_tag="OO130_001561"
     CDS             complement(2979..3911)
                     /gene="thrB"
                     /locus_tag="OO130_001561"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HCR5365019.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(3913..6375)
                     /gene="thrA"
                     /locus_tag="OO130_001562"
     CDS             complement(3913..6375)
                     /gene="thrA"
                     /locus_tag="OO130_001562"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HCR5365020.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFVQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTSGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTNVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHLLRHAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGACRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(6400..6519)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(6455..6520)
                     /gene="thrL"
                     /locus_tag="OO130_001563"
     CDS             complement(6455..6520)
                     /gene="thrL"
                     /locus_tag="OO130_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HCR5365021.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(6734..7420)
                     /locus_tag="OO130_001564"
     CDS             complement(6734..7420)
                     /locus_tag="OO130_001564"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCR5365022.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLVESLHDVDFTVATTARSRAKYHYYATPVELVLLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPTKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(7820..7960)
                     /locus_tag="OO130_001565"
     CDS             complement(7820..7960)
                     /locus_tag="OO130_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5365023.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            8056..8772
                     /gene="arcA"
                     /locus_tag="OO130_001566"
     CDS             8056..8772
                     /gene="arcA"
                     /locus_tag="OO130_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HCR5365024.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(8832..10184)
                     /gene="creD"
                     /locus_tag="OO130_001567"
     CDS             complement(8832..10184)
                     /gene="creD"
                     /locus_tag="OO130_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HCR5365025.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNIILGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLQLNMALNLSGTGDLSVVPAGRNS
                     EMPLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDWATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTMDDQLRIWK
                     "
     gene            complement(10242..11666)
                     /gene="creC"
                     /locus_tag="OO130_001568"
     CDS             complement(10242..11666)
                     /gene="creC"
                     /locus_tag="OO130_001568"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HCR5365026.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDSQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPELGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTVVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLEQALGNLLDNAIDFTPESGRITLS
                     AEVDQEHVALKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(11666..12355)
                     /gene="creB"
                     /locus_tag="OO130_001569"
     CDS             complement(11666..12355)
                     /gene="creB"
                     /locus_tag="OO130_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HCR5365027.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QLVPDVMILDVGLPDISGFELCSQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSSPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDTHIKTLHAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(12368..12841)
                     /gene="creA"
                     /locus_tag="OO130_001570"
     CDS             complement(12368..12841)
                     /gene="creA"
                     /locus_tag="OO130_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HCR5365028.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            13052..13921
                     /gene="robA"
                     /locus_tag="OO130_001571"
     CDS             13052..13921
                     /gene="robA"
                     /locus_tag="OO130_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HCR5365029.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(13918..14565)
                     /gene="gpmB"
                     /locus_tag="OO130_001572"
     CDS             complement(13918..14565)
                     /gene="gpmB"
                     /locus_tag="OO130_001572"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HCR5365030.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAARRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            14617..15138
                     /gene="yjjX"
                     /locus_tag="OO130_001573"
     CDS             14617..15138
                     /gene="yjjX"
                     /locus_tag="OO130_001573"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HCR5365031.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENTSQRGEARSA
                     TLPLPAVILQKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVYQAL"
     gene            complement(15223..15549)
                     /gene="trpR"
                     /locus_tag="OO130_001574"
     CDS             complement(15223..15549)
                     /gene="trpR"
                     /locus_tag="OO130_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HCR5365032.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(15639..17576)
                     /gene="sltY"
                     /locus_tag="OO130_001575"
     CDS             complement(15639..17576)
                     /gene="sltY"
                     /locus_tag="OO130_001575"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HCR5365033.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            17787..19454
                     /gene="ettA"
                     /locus_tag="OO130_001576"
     CDS             17787..19454
                     /gene="ettA"
                     /locus_tag="OO130_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HCR5365034.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(19761..20993)
                     /gene="nadR"
                     /locus_tag="OO130_001577"
     CDS             complement(19761..20993)
                     /gene="nadR"
                     /locus_tag="OO130_001577"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HCR5365035.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIDTVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(21014..22396)
                     /gene="radA"
                     /locus_tag="OO130_001578"
     CDS             complement(21014..22396)
                     /gene="radA"
                     /locus_tag="OO130_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HCR5365036.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(22445..23413)
                     /gene="serB"
                     /locus_tag="OO130_001579"
     CDS             complement(22445..23413)
                     /gene="serB"
                     /locus_tag="OO130_001579"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HCR5365037.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            23519..24163
                     /locus_tag="OO130_001580"
     CDS             23519..24163
                     /locus_tag="OO130_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HCR5365038.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            24191..25207
                     /gene="lplA"
                     /locus_tag="OO130_001581"
     CDS             24191..25207
                     /gene="lplA"
                     /locus_tag="OO130_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HCR5365039.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTAEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEAITKAFFAHYGERVEA
                     EIISPDKTPDLPNFAEIFARQSSWEWNFGQAPAFSHLLDERFSWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPDQEKELRELSTWIA
                     GAVR"
     gene            25239..25388
                     /locus_tag="OO130_001582"
     CDS             25239..25388
                     /locus_tag="OO130_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5365040.1"
                     /translation="MIDLENQEREIINLMLSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLK"
BASE COUNT         6134 a         7029 c         6333 g         6013 t
ORIGIN      
        1 gcctgcatgg ctattaccaa tttgaccgcc attttactgc tctcgcctgt ggttcatacc
       61 attgccagtg attatctacg ccagcgtaaa ctcggcgtgc gcccggtgtt tgatccgttg
      121 cgttatccgg atatcggtcg ccagctttct ccggacgcgt gggatgatgt ttcgcaggag
      181 taatcacaac tatcgatcaa ctcattctct ttttttgcta aagtcggcat aaatttcctg
      241 caaggactgg atatgctgat tcttatttca cctgcgaaaa cgcttgatta ccaaagcccg
      301 ttgaccacca cgcgctatac gctgccggag ctgttagaca attcccagca gttgatccat
      361 gaagcgcgga aactgacgcc tccgcagatt agcacgctga tgcgcatcag cgacaaactg
      421 gcgggtatca acgccgctcg ctttcatgac tggcagccag atttcacgcc ggcgaatgcc
      481 cgccaggcga ttctggcgtt taaaggtgat gtctacaccg gcttgcaggc cgaaaccttc
      541 agcgaagacg atttcgattt tgcccaacag catttgcgaa tgctttccgg cttgtatggc
      601 gtactgcgcc cgctcgattt aatgcagcct tatcgtctgg aaatggggat ccgtcttgag
      661 aatgcccgag ggaaagatct gtatcaattc tggggagata tcatcaccaa caagctgaac
      721 gaggcgctcg cagcacaagg cgataatgtg gtgattaatc tggcgtcaga tgagtatttt
      781 aaatcggtga agccgaagaa attgaatgcc gagattatca agccggtgtt cctcgatgag
      841 aagaacggca aatttaagat catcagcttc tacgctaaga aagcacgcgg tctgatgagt
      901 cgtttcatta ttgaaaatcg gctgaccaaa ccagagcaac tgactggttt taatagcgaa
      961 ggttacttct ttgatgaagc ttcctccagc aatggcgaac tggtgtttaa acgctacgag
     1021 cagcgttaag tttgcagtca atgccggatg cggcgtgaac gccttatccg gcctacaaaa
     1081 gcatgcagat tcaatatatt gcagaattca cgtaggcctg ataagacgcg ccagcgtcgc
     1141 atcaggcgct gaatgccgga ttgttacccg gcatttgtca tttagcggtg atgtttgcct
     1201 ggaccatgac cgccgtgatg atcatgagga gctttcttat ggtggcgcgg cggtggcggc
     1261 ggtccgtgtg ggtgccagcg attgcctcgc cattcataat gttgtttcca ccagccgtgg
     1321 tcgcgccagt gacctccatc ccagtaatag ccacgattat cacgattgcc tatctgtaat
     1381 tttactgacg ggactaacgt aatttccgca gcctgtgctg ccatgggagc gaccagaacc
     1441 agggaaagtg cgagtacgat agattgcatc tttttcacag gttactcctt ttatcattgc
     1501 ccgttatggg tcgatatccg gagattacgc gatgagaacg acggttgtta ttctccgcaa
     1561 tcctaatctc taagttaccg catttattga gaatatctcc tttttccctg tcgtttttct
     1621 ccataatttc ttcataaaaa agccgggctg cataaaagca aacccggcct gattgagata
     1681 atgaatagat tttactgatg attcatcatc aatttacgca acgcagcaaa atcggcgggc
     1741 agattatgtg aaagcaaggg taaatcagca cgttctgcca gctcttttgg cagatccaac
     1801 gtttcaccga gaatcgcttc cacgctctct ttaaatttcg ccggatgcgc ggtgccgagg
     1861 aacaagccat attcgcctgg attcaactgg tcacgcagcg cacgatacgc tacggcagcg
     1921 tgcggctccg aggtgtagcc cagttctttt aactcacgca ttgtctgttg cgtggtttca
     1981 tcatccacgg ctgcataacc cagctctttc agttgccaga ttttgcggcg gaacaactct
     2041 tccacacgcg gccagttgtt cggctgactc acgtccatcg cgttggataa cgtcgcctga
     2101 gtcgctttgg gtgaccactg accgtcgtgc aggaaacgtg gcacggtatc gttcacgttg
     2161 gtcgcagcaa taaaacgttt caccggcaga cagagtgact tcgccagcag acccgccgtc
     2221 aaatcgccga agtttccgct tggcaccgag acaaccagct ggttgcgcgc ttcctgcggc
     2281 agctgcgcaa ctgcttcaaa gtagtagcaa atctgcgcca gcaaacggct gatgttaatc
     2341 gagttagctg agtttaaccc cagcgccact ttcagctctt catcatcaaa cgcctgcttc
     2401 accagcgcct gacaggcatc gaaatcgccg tcgatggcaa cagtttcgat attgccgccc
     2461 aatgtacaga acagtttttc ttgcagtgga ctgattttgc ctcgtggata gaggataacc
     2521 actttcacat tcggtaaacc gtagaaagca tgagccactg ccgctccggt atcaccggag
     2581 gtcgcggtca gaatggtcac tggcttatcg cccgcaatat gggtcagcat ttgtgccata
     2641 aagcgaccgc cgaaatcttt aaatgccagc gttggcccgt ggaacaattc cagacaaccg
     2701 acatcgcttt caacattggc gaccggagcc gggaaggcaa acgccgcgcg cacgcgctct
     2761 tccaggattt cctgcgggat ttcatcacca ataaacgccg agaggatctt cgcactgcgg
     2821 gtgacaaaat ccagcttcag catctcatca atttcagtca ggctgaattc cggcaggtcg
     2881 tgcggaaaaa acagcccctg atttttgccc aacccctggg ttacggcttg cgcaaagctg
     2941 acctgctcat tgtgatcttt cagattgtag agtttcattt agttttccag tactcgtgcg
     3001 cccgccgtat ccagccggca aatatgaaca aaaccttcct gattttgcag atagttctta
     3061 cccaaccagt cggcaacgcg ctgggcggta tccggcttgt cacacagagc gaacaaggtc
     3121 gggccggagc cggagatacc gctcgctacc gcgccgattt ccgcaaccgc ctgccgcgcc
     3181 tgccggaagc caggcagtaa ccgttcacgg tagggttcag cgataacatc tttcatcagc
     3241 ttcgcggcaa gctcaggctg acgggaatag caggcgtgaa tgaagccagc cagatgtcgc
     3301 ccgtgcgcaa tgcaatcctg gcggcgatac tgcgccggta aaatagccct ggcttctgcc
     3361 gttgagactt taatccccgg atacgccagc acccacagcc actcatcaaa ccctggcact
     3421 tgctggctga tgatgtcgtt ttcttcgatc atcaactgca taccaccaag aaaacacggt
     3481 gccacgttgt cgtaatgaat gctgccggag attcgtcctt ccagctcgcc catcaaagcc
     3541 agcaaacgag tgtcattaag cggcttgcca cagtgttcat tcatcgccat cagcgccgcg
     3601 accaccgaac aggcgctgga gcctaagccc gaaccgattg gcatattctt ttccagagtc
     3661 atcgccactg gaatttgctt gccaagctcc tggcaaaaac gctcccagca ctgataaacg
     3721 atattttccc gtggttctga cggcagctta tcggcaaagc gtccgaggtt gttgagactg
     3781 aatgtctctg ccgcctcaac cgtgactaca tctccgagca atgcaccatc aacaggtgtc
     3841 accgccgccc cgagcacatc aaacccgacg ctcatattgg cactggaagc cggggcataa
     3901 actttaacca tgtcagactc ctaacttcca tgagagggta cgtagcagat cggcaaagac
     3961 accggcagct gtaacgtcat tgcccgcacc atatccgcgc agcaccaacg gcagcggctg
     4021 ataatagtgg ctataaaagg ccagggcgtt ttcgccattt ttcactttga acagcggatc
     4081 attaccatcc acttcggcaa tcttcacgcg gcaggcacca tcttcatcaa tattgccaac
     4141 atagcgcaaa acttttcctt catcacgggc cttcgccacg cgcgcggcaa agagatcgtc
     4201 gagctgtgac agattcgcca taaaagcggc aacatcaccc tcagcgttaa actctgcggg
     4261 cagcacaggt tcaatttcaa tatccgccag ctccagttca cgtcccgttt cacgagcgag
     4321 aatcaatagc ttacgcgcta catccatacc agaaagatca tctcgcggat ccggttcggt
     4381 ataacccatt tcccgcgcca gagtagtcgc ctcggagaaa ctcatgcctt cgtctaactt
     4441 gccgaagata taagaaagcg aacctgaaag aatgccggag aacttcatca attcatcacc
     4501 agcattgagc agattttgca ggttctcaat aaccggtaat ccagccccaa cgttggtgtc
     4561 atagaggaat ttacgccgcg atttttccgc cgcatgacgc aacagatggt agtaatccat
     4621 cgacgaggtg ttggcctttt tgttcggcgt gacaacgtgg aaaccttcgc gcaagaagtc
     4681 ggcatattga tccgccactg cctggctgga ggtgcagtca acaatgaccg ggttcagcag
     4741 atgatattct ttcacgaggc gaattaagcg cccgagatta aacggctctt tggcttgcgc
     4801 cagttcttcc tgccagtttt ccagatttag gccatgcaca ttggtgagca gtgccttcga
     4861 gttggcaaca ccgcagacac gtaagtcgat atgtttattc ttcagccagc tttgttgacg
     4921 cttcagttgc tccagcagcg caccgccaac gccaccgacg ccaatcacaa acacttcgat
     4981 aacctgatcg gtattgaaca gcatctgatg agtaacgcgc acgccagtgg tcgcatcatc
     5041 gttatttacc acgacagaga ttgagcgttc agaagatccc tgagcaatgg cgacaatgtt
     5101 gatattggcg cgggccagcg cggcaaagaa tttcgccgag atcccacgca aggtgcgcat
     5161 accatcacct accaccgaga taatggccag ccgttccgtc accgccagcg gctccagtaa
     5221 gccttctttc agttccaggt agaactcttc ctgcattgcc cgttcagctc gcacacagtc
     5281 gctttgtgga acgcagaaac tgatactgta ttcggaagat gattgcgtaa tcagcaccac
     5341 ggaaatacgg gcgcgtgaca ttgcagcaaa gacgcgcgcc gccatgccga ccattccttt
     5401 catccccgga ccagaaacgc tgaacattgc catattattc agattggaaa tgcctttgac
     5461 cggtaattcg tcttcatcac ggctggcacc aatgagcgta cctggtgctt gaggatttcc
     5521 ggtattttta atcaggcaag ggatctggaa ctgggcgatg ggggtaatgg tgcgggggtg
     5581 aagaacttta gcgccgaagt aggaaagctc catcgcttcc tggtaggaca tcgacttcaa
     5641 caacctcgca tcgggcacct gacgcgggtc gcaggtatag accccgtcaa cgtccgtcca
     5701 aatctcgcaa caatcggcgc gtaaacaggc agccagcacc gccgcggagt agtcggaacc
     5761 gttacgtcca agtaccacca gttcgccttt ttcattaccg gaggtgaaac ctgccatcaa
     5821 caccatgtga tcagccggaa tgcggcttgc cgcaatacgg cgggtggact cagcaatatc
     5881 gacggtagat tcgaggtaat gccccactgc cagcagtttt tcgaccggat cgataacggt
     5941 aacgttgtga ccacgagctt ctaacacgcc ggccataatg gcgatcgaca ttttctcgcc
     6001 acggcaaatc agcgcagcgt tgatgctatc cgggcactgc cccaacaaac taatgccatg
     6061 caggacatgt tttatttgga caaattcctg atcgacgaaa gttttcaatt gcgccagcgg
     6121 gaaccccggc tgggcggcgg cgagtcccgt caaaagttcg gcaaaaatac gttcggcatc
     6181 gctgatattg ggtaaagcat cctggccgct aatggttttt tcaatcatcg ccaccaggtg
     6241 gttggtgatt ttggcggggg cagagaggac ggtggccacc tgcccctgcc tggcattgct
     6301 ttccagaata tcggcaacac gcagaaaacg ttctgcattt gccactgatg taccgccgaa
     6361 cttcaacact cgcatggttg ttacctcgtt acctttggtc gaaaaaaagc ccgcactgtc
     6421 aggtgcgggc ttttttctgt gtttcctgta cgcgtcagcc cgcaccgtta cctgtggtaa
     6481 tggtgatggt ggtggtaatg gtggtgctaa tgcgtttcat ggatgttgtg tactctgtaa
     6541 tttttatctg tctgtgcgct atgcctatat tggttaaagt atttagtgac ctaagtcaat
     6601 aaaattttaa tttactcacg gcaggtaacc agttcagaaa ctgctatcag acactctttt
     6661 tttaatccac acagagacat attgcccgtt gcagtcagaa tgaaaagctg aaaaatactt
     6721 actaaggcgt tttttatttg gtgatatttt tttcaatatc atgcagcaaa cggtgcaaca
     6781 ttgccgtgtc tcgttgctct aaaagcccca ggcgttgttg taaccagtcg accagtttta
     6841 tgtcatctgc cactgccaga gtcgtcagca atgccatgac tcgttcgcgt aaagcttgca
     6901 gttgatgttg gtctgccgtt gtgtcacttt tcgtcggttg ttgtattaat gttgctaatt
     6961 gatagcaata gaccatcacc gcctgcccca gattgagcga aggataatcc gccaccatcg
     7021 gcacaccagt aagaacgtca gccaacgcta actcttcgtt agtcagcccg gaatcttcgc
     7081 gaccaaacac cagcgcggca tggctcatcc atgaagattt ttcctctaac agcagcacca
     7141 gttctactgg cgtggcgtag taatgatatt tcgcccgact gcgcgcagtg gtggcgacag
     7201 tgaaatcgac atcgtgtaac gattcaacca gtgtcgggaa aactttaata ttatcaataa
     7261 tatcaccaga tccatgtgcg acccagcgag tggctggctc cagatgtgcc tgactatcga
     7321 caatccgcag ttcgctaaaa cccatcgttt tcattgcccg cgccgctgcc ccaatatttt
     7381 ctgctctggc gggtgcgacc agaataatcg ttatacgcat attgccactc ttcttgacca
     7441 aatgaccgcg aacagggtga tcactgtcaa cttattacac ggtgcgaatt tacaaatcct
     7501 taacgtaagt cgcagaaaaa gccctttact tatcttaaaa aaggctaaac tatttcctga
     7561 ctgtactaac ggttgaattg ttaaaaaatg ctacatatcc ttctgtttac ttaggataat
     7621 tttataaaaa ataaatctcg acaattggat tcaccacgtt tattagttgt atgatgcaac
     7681 tagttggatt attaaaataa tgtgacgaaa gctagcattt agatacgatg atttcatcaa
     7741 actgttaacg tgctacaatt gaacttgata tatgtcaacg aagcgtagtt ttattgggtg
     7801 tccggcccct cttagcctgt tatgttgctg ttaaaatggt taggatgaca gccgtttttg
     7861 acactgtcgg gtcctgaggg aaagtaccca cgaccaagct aatgatgttg ttgacgttga
     7921 tggaaagtgc atcaagaacg caattacgta ctttagtcat gttacgccga tcatgttaat
     7981 ttgcagcatg catcaggcag gtcagggact tttgtacttc ctgtttcgat ttagttggca
     8041 atttaggtag caaacatgca gaccccgcac attcttatcg ttgaagacga gttggtaaca
     8101 cgcaacacgt tgaaaagtat tttcgaagcg gaaggctatg atgttttcga agcgacagat
     8161 ggcgcggaaa tgcatcagat cctctctgaa tatgacatca acctggtgat catggatatc
     8221 aatctgccgg gtaagaacgg tcttctgtta gcgcgtgaac tgcgcgagca ggcgaatgtt
     8281 gcgttgatgt tcctgactgg ccgtgacaac gaagtcgata aaattctcgg cctcgaaatc
     8341 ggtgcagatg actacatcac caaaccgttc aacccgcgtg aactgacgat tcgtgcacgc
     8401 aacctgctgt cccgtaccat gaatctgggt actgtcagcg aagaacgtcg tagcgttgaa
     8461 agctacaagt tcaatggttg ggaactggac atcaacagcc gttcgttgat cggccctgat
     8521 ggcgagcagt acaagctgcc gcgcagcgag ttccgcgcca tgcttcactt ctgtgaaaac
     8581 ccaggcaaaa ttcagtctcg tgctgaactg ctgaagaaaa tgaccggccg tgagctgaaa
     8641 ccacacgacc gtactgtaga cgtgacgatc cgccgtattc gtaaacattt cgaatctacg
     8701 ccggatacgc cggaaatcat cgccaccatc cacggtgaag gttatcgctt ctgtggtgat
     8761 ctggaagatt aatcggcttt accaccgtca aaaaaaacgg cgctttttag cgccgttttt
     8821 atttttcaac cttatttcca gatacgtaat tgatcgtcca ttgtaacttc tttactggct
     8881 ttcattttcg gcagtgaaaa cgcataccag tcgatattac gagtcacaaa catcatgccg
     8941 gccagcgcca ccaccagcac actggttccc aacaacagcg cgctatcggc agagttgagc
     9001 agtccccaca tcacaccatc cagcaacaac agcgcgaggg taaacaacat gctgttgcgc
     9061 cagcctttca ataccgcctg caaataaata ccgttcatta acgccccaat cagactggcg
     9121 attatccatg ccacggtaaa accgatatgt tcagaaagcg ccagcaagag caaataaaac
     9181 atcaccaatg aaagccccac cagcaaatat tgcattgggt gtaaacgttg cgcggtgagc
     9241 gtttcaaaaa caaagaacgc cataaaagtc agtgcaatca gcagaatggc gtacttagtc
     9301 gcccagtccg ttaattggta ttgatcggct ggcgtcgtca ctgcgacgct aaacgcgggg
     9361 aagttttccc agccggtatc attgcctgaa gcaaaacgct caccgagatt attagcaaac
     9421 cagctgcttt gccattgcgc ctgaaaacct gactcgctaa cttcccgttt ggctggtaga
     9481 aaatcaccta aaaaactggg atgcggccag ttgctggtta agggcatttc gctattacgc
     9541 ccggcaggca ccacagaaag atcgccggta ccgcttaaat tcagtgccat attcagctgc
     9601 aggttctgct tccgccagtc cccttcgggt aaagggatat gcacgccctg cccgccttgc
     9661 tctaacccgg taccgggttc aatggtcagt gccgttccgt taacttcagg cgctttcacc
     9721 acaccaatac cacgcgcatc cccgacgcta atcacaataa agggcttgcc taagatgata
     9781 tttggcgcgt tgagctcgct aagacgcgaa acatcgaaat cggcttttag cgttaaatca
     9841 ctgtgccaga cctgaccggt ataaatccct atcttgcgtt cttccacgtt ctgattgcca
     9901 tcaaccatca atgactcagg taaccaaaaa tggataaaac ttcgtttccg ctccacggtt
     9961 ttatcctctt cctgcaccgt ataaagctcg gtcacaggaa tagcgataag cggcccaacg
    10021 agtttttgcg gcccgctggt actttggcga atcgcatctt ccacatcgct acggtaatcg
    10081 gcacgttcga caatcacttg ccgaatcagc attatcggaa tcaacaacag caatactgca
    10141 ccaaacaagg tggtcatttt ccagaacagg ggggatttca acatagtctt ctcctttgca
    10201 atggcagcag gatacgaagg ctatgtggga agaatttgaa gctatgtgaa gtgacggtga
    10261 agtcgaagcg aggccagcac gccaccttcc tgcacgttgc gcagcgtgac ttcgccgtta
    10321 aacaaacggg cgacctcact gacgaacgcc aaccccagac cgctgctttt ttgcccattt
    10381 gcacgcggca aagagtaaaa gcgttcaaaa atacgtgaca gcgcgtagtc aggaatacca
    10441 ctaccggtat ccagcacctt aagggcgacg tgttcctgat ccacttcggc gcttagcgtt
    10501 atgcgaccgc tctcgggggt aaaatcgatg gcgttatcca gtaaattccc cagcgcctgc
    10561 tccagtaacg ccggttcagc agcaacatta acctcagtgg gcataacatg tagagtgatg
    10621 tttttttctg ccaactgcac ggtgcgcgct tcgctgacgc ggcgaaataa tgccgccaca
    10681 tcaacaacag tcagaacgac ttcctgacga ttctccagtc ttgcctggcg tagtaacgtt
    10741 tctaccaatg cctgcatacg cgcattttgc gtcagaatgt tgtcggtaaa acgagccacc
    10801 acttccggtg gcggaccttc gcgtaaaatt tccgccgcgc cacgaatcgc cgccagtggg
    10861 ctttttagct catgggttaa cgcataaaca tactgctcaa tatagttttt cccttccagc
    10921 ttcacgcgca tactttccag cgcctgcgca agcttacgca actcgctact gccgagttca
    10981 ggaagaggaa cgggcttatt gtcagtgacg gaatcggcat agcgagtgag ccgggcaatg
    11041 gagcggttga tccaccaaac catgcctgcg ccaatcacca gtgcaatccc cagcaaaatg
    11101 gcgctggccc ataaaattcg ccgctcgcta cgcttaatga ccggagccat cgccgcgttc
    11161 ggtttgccta cgctcaaaac gccaataagc cgcgagccgt ccataatcgg cgcggcaaca
    11221 tacatcaccg aactttcggg atcggcagga ttttgcaacg tgctgcgcgc accatactga
    11281 ccacgcaacg ttagccagac gtcattccag cgcgaataat cctgcccaac ggctttattt
    11341 gccgaatcga acaatacttt accctgcgaa tcggtcatat agacatgata ttcattgcgc
    11401 actttgttaa tgccaccgat attggcgcga aacggacgat gttgtagctg attaaacgcc
    11461 tgcgccagtt gcccatgcgt tgggtcccca gagagcaaat cgggacgcgc cagctccgcc
    11521 agcaacgttg cggtgtcgat caacgtcccc tccgttgctc ttcgcacgcc cggtttaact
    11581 tctttgacaa aaatagccag tacgaaccag gccgccaccg ccaccagtaa aaaatagccc
    11641 agcagcaacc gcatgccgat acgcattaca ggcccctcag gctatatccc atgccgcgat
    11701 gagtattaat cggtgaaaga tcggggttga tggcacgcag cttggcatgc aacgttttaa
    11761 tgtgggtatc aacggtgcga tcgtaggtgt cctgcgcatc ttcccacacg ctatccatca
    11821 gttgctggcg ggaccagacg cggcctggcg acttgagtaa cgtcttcagc aataaaaact
    11881 cataccgcgt cagcgttaat ggcgtgtcaa accagctgat ctgcgccgcg ggttcattca
    11941 attcaaaatg accaatacgg atgacgggag acggcgacga gaacttcttc acccgacgca
    12001 gtaaggtgcg caccctggcg cacacttcgc ggggtgaaaa cggtttagcc acatagtcgt
    12061 cagcgccaat ttccagccca agcaggcgat cgacctcttc acttcgggcc gtcaggaaca
    12121 gtacaggtaa cgccggatgg agcgccagta actggctgca caattcaaag ccgctgatat
    12181 caggcaaacc aacgtcgaga atcatgacgt cgggaaccag ctggcgagct ttatccagca
    12241 ccggcaagcc tcgctcaaag acctcgacgg caaaaccttc ctgctgcaac atgtagacca
    12301 gcgtgtcggc tatcccttgc tcatcttcca ctaaccagac cgtttcccgt tgtataaatc
    12361 gcctctgtta ttgccgccac ggcatgacag gaaccgcgct aatcgcgttt ttcggcgaac
    12421 cttctacaac tttgtcggag taagccagat aagcgagcgc gttgcgtttg gcgtcataaa
    12481 agcgcacgac ctgtaacgac ttaaagacca gggacgtgcg ttttttgaat actacctcgc
    12541 cctgagcttt gccgttttta atacgatccg acagttcaat cggcccgact tgctgacaag
    12601 aaatagccgc atcggaggta tcttccgcca gacccaatcc ccctttaata ccacctgttt
    12661 tcgcccggct cacataacag gtgacatttt tcacatcggg atcgtcaaag gcttccacaa
    12721 caattttgtg atccgggccg atcattttaa atacggtgtc gaccgaacca atctcttctg
    12781 catgagccaa tggccccagc attatcaggc ttaaagacag gatcaaatgc ttgtatttca
    12841 tattgttacc atttttatct attacacgag caattagttc gtcacggtaa ataacatatt
    12901 gattaagaac aaaatctcaa ttcttttatt tccgtcaagc cctacaacat actctactaa
    12961 gaaaaaaaca ctgaatgcta aaacagcaaa aaatgctatt atccaattac ctgatgtcag
    13021 gtgctcgttg ttgaaaggat gaggatattt tatggatcag gccggcatta ttcgcgacct
    13081 tttaatctgg ctggaaggtc atctggatca gcccctgtcg ctcgacaatg tagcggcgaa
    13141 agcaggttat tccaagtggc acttacagag aatgtttaaa gatgtcactg gccatgctat
    13201 tggcgcgtat attcgtgctc gtcgtttgtc gaaatcggcg gtcgcactac gcctgactgc
    13261 gcgtccgatt ctggacatcg cgctgcaata ccgcttcgac tctcaacaga catttacccg
    13321 cgcattcaag aagcagtttg cccagactcc tgcactttac cgccgttctc ctgaatggag
    13381 cgcctttggt attcgcccgc cgctacgtct gggtgaattc acaatgccag agcacaaatt
    13441 tgtcaccctg gaagatacgc cgctgattgg tgttacccag agctactcct gttcgctgga
    13501 gcaaatctct gatttccgcc atgaaatgcg ttatcagttc tggcacgatt ttctcggcaa
    13561 cgcgccgacc attccgccag tgctctacgg cctgaatgaa acgcgtccga gtcaggataa
    13621 agacgacgaa caagaggtat tctataccac cgcgttagcc caggatcagg cagatggcta
    13681 tgtactgacg gggcatccgg tgatgctgca gggcggcgaa tatgtgatgt ttacctatga
    13741 aggtctggga accggcgtgc aggagtttat cctgacggta tacggaacgt gcatgccaat
    13801 gctcaacctg acgcgccgta aaggtcagga tattgagcga tactacccgg cagaagacgc
    13861 caaagcggga gatcgcccaa ttaatctgcg ctgtgaactg ctgattccta tccgccgtta
    13921 acgctgcagc tcatctaatg caggggcgtc cagatgcgag atgtcccctg cggtttcaac
    13981 gacccagcca gacgccagcc acaggctttc ctgataatcc acgcgcgaaa tagaacagtt
    14041 acgcagacgt aagcggcgcg ctgcccatgc tggtaatccg agaatcgtac tcaccagaca
    14101 tcccagtgca ataccatggc tcaccagcag cggtcggctt ccctgcggta agtcccggca
    14161 agattccagt gccgcattga cgcgatcgct gagctcctgc attgactcgc cttcaggaat
    14221 acgcccgtca acggtgccat tgaccagctg ccgacgccag ttctcttctt cttcggtcag
    14281 agaatcgata tgtctttttt ccagcacacc catgtttaat tcacgcaggc gagaatcaaa
    14341 gatgatgtca cagccgcagg cctgggcgat gatttccgcc gtacgccggg tgcgtcctaa
    14401 atcgctactg ataatatgcg taatgccaag ctctttggca cgggttgcca cctgcatcgc
    14461 ctgttgctca cctttggcgg tcagcgggct gtcagactgg ccctgaatac gtcgctcggc
    14521 gttccactgc gtttcaccgt ggcggactag gtatacctgt aacatgcttt ttttccgtat
    14581 actgctgtaa cgttaatttt ctgagtaatg ctgattatgc accaagttgt ctgtgcgacc
    14641 accaatcccg ctaaaattca ggccattctg caggcatttc acgagatctt cggcgaagga
    14701 tcctgccata ttgcatccgt tgccgtcgag agcggtgtac cggaacagcc ctttggcagt
    14761 gaggaaacgc gcgctggcgc acgaaatcgg gtagcaaatg cccgccgttt acttccagag
    14821 gctgattttt gggtggcgat tgaagctggc atcgatggtg acagcacttt cagttgggtg
    14881 gtgattgaaa acaccagcca gcgcggcgag gcgcgttcgg ctactttgcc gttacctgcg
    14941 gtgattctgc agaaagtgcg tgaaggtgag gcgcttggtc ccgtgatgtc gcgttatacc
    15001 ggtattgatg agattggtcg taaggaaggg gcgatcggcg tgtttaccgc cgggaaactc
    15061 actcgcgcca gcgtctatca ccaggcggtg atcctggcgc tgagtccgtt tcacaatgcc
    15121 gtgtatcagg cattgtagga cggataaggc gttcacgccg catccggcat tcggtgcacg
    15181 atgcctgatg cgacgctgcc gcgtcttatc aggcctacaa aatcaatcgc ttttcagcaa
    15241 cacctcttcc agccactggc gcagctccac gggcgcggct ttcaggctgt tagatccacg
    15301 cgtaatcgtc gcgatgcccg cgccgagttc attttttaac tcacgctgac tcatttcgcc
    15361 acgcaacagc tcttcgacaa tacgcacgcg agtccccaac gcttcgcgct catctggcgt
    15421 cagcatcagg tttaacaacg gtaaatggag atcgttttgg taggcattct taagcaggtc
    15481 gacaaaacgt aaccactcct ggtgacgctg ttctgccatc gctgctgaat agggtgattg
    15541 ttgggccata atatgtcgcc attgttagcg ggggaagcaa aatgcctccc ccggttgtac
    15601 tcgctaaaga gtacgatagc atatcataaa cgtgcggatc agtaacgacg tccccattcc
    15661 gtggcgctca tcaacgtcgg tttatccccc atgaaataac ggtagtaagc gtcataagcc
    15721 agcacgttct tcacataacc gcgcgtctcg gagaacggaa tactctcgac aaatgccaca
    15781 gcgtcgatac gcccggcgct gttgccaagc caggttcgca cccgtccagg tccggcgtta
    15841 taagctgctg aggagaaaat acggttattg ccaaactgct gataaacata ttgcaggtaa
    15901 ctggtgccaa tgttgatatt cgtttccgga tccagcaatt gcccagggct gctataacca
    15961 ggaatagaga acatcttcac cgtatgggtc gctgtaccag gcataatctg catcaggccg
    16021 ctggccccta ccggtgattt cactttcgga ttccaggcgc tctcctgacg ggcaatcgcc
    16081 atcgcatagc tttgcgggat ctctttccca ctggtgtaac gtttgaaaag atcgttgtaa
    16141 gccagcggga atcgctcttc cagatgatcc caaagcttcc cggcgatcgt tgcctgaacg
    16201 ctaagatccc accattggtt attgaaagca taccgagcca gttgagcctg ctctgttttt
    16261 gacttgctct tcaccagatt ggcccactcg ctacgcgcgg tgttatcgag attccagtac
    16321 atcaactcgc gcacgcgcgc catctccggc ccctgagtca gggcgctgtc aacattctgc
    16381 ggcgctttat caatcttcag ctcatactct tctccgatgc gttgtgctgc aaccatcggg
    16441 tagaaaccac gctgttgcat gagttgatgc aaaatctctt tcgcttcagc ttcacgtccg
    16501 cgttccagca ataaatccgc ctgccagtaa cgccattcat ctttctcttt tgcttccatc
    16561 ggcagacgcg ccagccaggt attcaggccg cgacgatcgc cggtgccaag tgccattcgc
    16621 acacggcgtt caatcaacga cgtggattgc gagcgcataa tggcgtcatc gcgccatttc
    16681 gcctgctcgt cggtgacatc gttgcccatc aaacgccagg cgacgatatc gcgcagctcc
    16741 tgaatctgat cttcattaag ctgctgcgcc tgggcaagcg acgggatcat cagccgtgca
    16801 ttttcagcat cctgccgcgc cacactggca aacgccaccg ctgccatttg acgggtaaaa
    16861 tcggtcgctc cggttgtacg cgcgaaggtc agtaccgtat tagggttgtt cgccagtgaa
    16921 atgattgccg aggcgatagt ctggtaatcg gcaggcatct gccctgccag cactgttacc
    16981 aggccagtgt tacccgcttt catcgccaga cggatacgct ctaaatacgc cagcggatct
    17041 tgtttacctg acgcacgcca gacgctaaat aacttgtcac aggcgttagg ctggctcttg
    17101 ccggttagcc acagctcttt cgccccttgc caggcttctt cactctgccc ggtgttccat
    17161 ttcgcatagt agtaattaca ttgcgcttcg gtagttccgg gcttttccgg gctaaaggct
    17221 aacaagccac gccagtcttc acgccgcgcc agttcattga cgaaacgaga ttgcagcgtg
    17281 cgagcgggag gaagcgtggg gttagcgcga acaaagttag tgaccgtcac cgccggttga
    17341 ttcatcagat catcggtgat ctggcggtat tccaggtagg gataaagcgg ataatccttc
    17401 agtccaggca tcatttgttc gaccacatcc atttgtcgat tatcccaggc ctgcttgatt
    17461 tgcgcgtaac ggctacgctg ctcatccagt gagtcggctc gcgccacgct gctgaccgtc
    17521 agcagacaga caccggcagc caacagccgc caggtaactt gtttggcttt ttccacaagc
    17581 gcatcctcta agtgtaaata catcaatgca gcgtcatgcc gcgtaataga tttatgctaa
    17641 ccagtcattg ccgtttacgc cacgttacgg acactttttt acttttactg cgaaggtgat
    17701 cgaccaactg ggattaggcg gggaaaaagg ctacacttcg cctttgaaaa ctcatcatca
    17761 cgataaacat agaggcgaag tccaacgtgg ctcaattcgt ttataccatg catcgtgtcg
    17821 gcaaagttgt tccgccgaaa cgtcatattt tgaaaaacat ctctctgagt ttcttccctg
    17881 gggcaaaaat tggtgtcctg ggtctgaacg gcgcgggtaa gtctaccctg ctgcgcatta
    17941 tggcaggcat tgataaagac atcgaaggtg aagcgcgtcc gcagccagac atcaagattg
    18001 gttacctgcc gcaggaaccg cagctgaacc cggaacacac cgtgcgtgag tccattgaag
    18061 aagcggtttc tgaagtggtt aacgccctga aacgcctgga tgaagtgtat gcgctgtacg
    18121 ccgatccgga tgccgatttt gacaagctgg ccgctgaaca aggccgtctg gaagagatca
    18181 ttcaggctca cgacggtcat aacctgaacg tacagctgga gcgtgcggcg gatgcgctac
    18241 gtctgccgga ctgggacgcg aaaatcgcta acctctccgg tggtgagcgt cgtcgcgtag
    18301 cgttgtgccg cctgctgctg gaaaaaccag acatgctgct gctcgacgaa ccgaccaacc
    18361 acctggatgc cgaatccgtg gcctggctgg aacgcttcct gcacgacttc gagggcaccg
    18421 tggtggcgat tacccacgac cgttacttcc tcgataacgt tgcaggctgg atcctcgaac
    18481 ttgaccgcgg tgaaggtatt ccgtgggaag gcaactactc ctcctggctg gagcagaaag
    18541 atcagcgcct ggcgcaggaa gcttcacaag aagcggcgcg tcgtaagtcg atcgagaaag
    18601 agctggagtg ggtacgtcag ggaactaaag gccgccagtc gaaaggtaaa gcacgtctgg
    18661 cacgctttga agagctgaac agcaccgaat atcagaaacg taacgaaacc aacgaactgt
    18721 ttattccacc tggaccgcgt ctgggcgata aagtgctgga agtcagcaac ctgcgtaaat
    18781 cctacggtga tcgcctgctg attgatgacc tgagcttctc gatcccgaaa ggggcaatcg
    18841 tcgggatcat cggtccgaac ggcgcgggta aatcgaccct gttccgtatg atctctggtc
    18901 aggaacagcc ggacagcggc accatcactt taggtgaaac ggtgaaactg gcatcggttg
    18961 atcagttccg tgactcaatg gataacagca aaaccgtttg ggaagaagtt tccggcgggc
    19021 tggatattat gaagatcggc aacaccgaga tgccaagccg cgcctacgtt ggccgcttta
    19081 actttaaagg ggttgatcag ggtaaacgcg ttggtgaact ttccggtggt gagcgcggtc
    19141 gtctgcatct ggcgaagctg ctgcaggttg gcggcaacat gctgctgctc gacgaaccaa
    19201 ccaacgacct ggatatcgaa accctgcgcg cgctggaaaa cgccctgctg gagttcccgg
    19261 gctgtgcgat ggttatctcg cacgaccgtt ggttcctcga ccgtatcgcc acgcacatcc
    19321 tggactacca ggatgaaggt aaagttgagt tcttcgaagg taactttact gagtacgaag
    19381 agtacaagaa acgcacgctg ggcgcagacg cactggagcc gaagcgtatc aagtacaagc
    19441 gtattgcgaa gtaatgcgta aaatgccgga tgcggcgtga acgccttatc cggcctacaa
    19501 accaagaaat ttcaatacgt tgtgaatctc caggcctgat aagacgcggt aagcgtcgca
    19561 tcaggcattg agcaccgaat gccggatacg gcgtgaacgc cttttccggc ctacaatagc
    19621 cttagtatcc gcaatttcgt agcgggtcac ccgtgctgat tcttttaaac cttgtttaat
    19681 ctcgacagcc tcttccagag aggttttcaa ctcatcaaaa aacttatttt atacctcgcc
    19741 tttgagccgt ttcatcgcgg ttatctctgc tcccccatca tctcccgcac cagctccacg
    19801 cagcgcagga aacggctgtc ataatcgtcc tcttcaaccc gcacgaattc gatattgttc
    19861 tcttccagca tctccaccag caagttctgg aactctttgc gatccaccga actgccgagg
    19921 ctgcgtaaac catccgccac ccacggcgtg ttgttctcca gcaggatcac cagatcgaaa
    19981 cggtattcat caatcaacgc ctgtacgaac ggatgctcac gcccttcgta ctttttgcag
    20041 aacgcctggg tagtgacaaa atcagtgtca ataaacgcca ctttattggc atatttcact
    20101 gcaaaatcaa tgtattgtgc gtggcccagc gcgattttat cgtaatcgga atactgcaat
    20161 gcgatctcat caccgccgag gtgtgaaaag acataatcgc gaccatattc ccacgcactg
    20221 gtggtattga agatattggc aagtttgttt accagggtgg atttaccgct cgactcgcca
    20281 ccaaggatcg ccacggtacg tacaaagaac ggcttcactt ccgtaggaat atattcccag
    20341 tagcggaacg ggttttcgcg gatctgcgca ccgctgatac tcataaaggt acgtttcgga
    20401 tcgaccagca ccgtatcgat ccccagatgt tccatatact gtggcgcatc ggcttcttcc
    20461 gaggtgtaga tcaggtccgg ctgaatcccc ttttcagcca taaacttttt gatgccgttg
    20521 ctccacacat cccagccgtg cggatacggc tccatgccct cctcgttgaa agcatgaatg
    20581 cgaatatttt tctgatattt aaaagtttgt aataaccagc gcagacgatc cggcaccgtg
    20641 ggctgctgcg acatggcgct gtcttcaaac aacgcgcggt cgcgggtatc gtcaaaaccc
    20701 ataatgatat gcagctcgtc aacctggcta caggcgcgct ggataaggta gatatgtccg
    20761 gtatgcagcg ggtagaactt accgaatacg acaccaatgg ttttcttctg tcggggaaat
    20821 tcaagcccca aaaaacggtg caacgcctcc agcttttgcg cgctggggct tttaattttg
    20881 gcattcagta actggcttaa ataccctttg gtcataccgc tggcatcagc tacctgctgt
    20941 agcgtgcagc cctgttgctt gatggcagtt ttcaggtaat caaatgacga catatctccc
    21001 tccgtatctc tcattataag tcgtcgaaca cgctaagcgc gtcggagagt tttttaacgc
    21061 caaaaatctg catcccttcc ggcgcttttt tcggtacgtt agccgccgga acaatcgccc
    21121 ggcgaaaacc gtgtttcgcc gcttctgaaa ttcgttcctg accgctgggc accgggcgga
    21181 tctcccctgc cagcccgact tcaccaaaca ccaccagatc ctgtggcagc ggtctgtcac
    21241 gcaggctgga aaccatcgcc agcagtaacg ctaaatcggc actggtttcg gttaccttca
    21301 cgccgccgac cacgttcaca aatacatcct gatcggccat ttgcagacca ccgtgacggt
    21361 gcaagacagc cagcaggatt gccagacggt tttgttccag ccccactgcc acgcggcgcg
    21421 gattcgccat catcgagtga tcgaccagcg cctgaatctc caccagcaac ggacgcgttc
    21481 cttcccacac caccatcacg gagctgccgg aggtcacttc atctccgcga cttaagaaaa
    21541 ttgccgaagg gttgctgact tcacgcagcc cctgttcggt catcgcgaag acgcccagct
    21601 cattcaccgc gccgaagcgg tttttatggc tgcgcaaggt gcgaaaacgg gagtcggcat
    21661 caccatccag aagcaccgaa cagtcgatac agtgttccag cactttaggg ccagccagcg
    21721 agccatcttt ggttacgtgc ccaaccatga caatcgccac accgcgcgtt ttggcgaagc
    21781 gcgtcagata agccgccgtt tcacgcacct gcgccacgct accaggcgat gattgtacat
    21841 ccgccatatg catcacctgg atcgagtcaa ttaccatcag tttcggttgc tcttcttcgg
    21901 caatcaggca gatctgttcg atgctggttt ccgacaacat attgagattg tcagtcggca
    21961 ggccaaggcg atgagcgcgc attgccacct gttgcagcga ctcttcgcca gtgacataca
    22021 gcgttttcat ctgctgggcc agtttgcaca gcgtttgcag tagcagcgtg gatttccccg
    22081 cacctgggtt accgccaatc agaatggcgc ttcccggcac cacgccgccg cctagtacgc
    22141 ggtcgaactc tttaaatccg gtggaaaaac gcggcagctc ttcaaggctg atatcggaga
    22201 gtttctggac ttttgccacc ccggcgctac cggcatagcc gctgagacgc tcgttacgcg
    22261 ccaccgttgg cgacgcagca agacgcacct cggtgatggt gttccaggca tgacaggcac
    22321 tgcactgccc ctgccagcgc ggataatcgg ccccgcattc attacaaaca aaggcgcgtt
    22381 ttggagcttt tgccacggtg tacctcgtta atgctgtgcc gcccgcagga tggcgggcga
    22441 gcaattactt ctgattcagg ctgccagaga ggatgcagaa tacccccatc aggtcagcgt
    22501 gacggatggt gacttccgcc ttttcattca cttttggctt ggcatggtag gcaatcccca
    22561 gccctgctgc tttgatcatc ggcaggtcat tagctccatc gccaatcgcc acggtctgcg
    22621 ccagcgggat ttcatactcc tgcgcgaggc gagtcaaagt tttcgctttg tactgcgcgt
    22681 ctacgatgtc gccgatcaca ttgccggtaa atttaccgtc catgatctcc agttcattgg
    22741 ctaccacggc ggtcaggcgc agcttgtcgc gcagatattc agcaaagaaa gtaaagccgc
    22801 cggaggcaat cgccactttc cagcccagcg tttccagctt gagcaccagt tgcgttaagc
    22861 ctggcatcag cggcagattt tcacgcacct gttgcagaat attggcgtca gcgcctttca
    22921 gcgtcgccac acggctgcgc aggctggcgg taaaatcgag ttcgccgcgc atcgcccgtt
    22981 cggttacttc cgccaccatc tcgcccgttc cggccagttt ggcaatttca tcaatacatt
    23041 caatctggat ggcggtggaa tccatgtcca tcaccagcaa acccggcgtg cgcaggtgcg
    23101 ggattttccc cagcggggcg acatccagtt gcgcttcatg ggccaggcgc gtagcccgtg
    23161 cggtgagtga acctgccaga cgaatcacct gataatcttc cacgcaccag gcggcaacaa
    23221 tcaccatcgc cgcacccagt ttgctctggt attgggtcag acgctgttta tccagcccac
    23281 gaccatacag cagccagccg ctacgacctg cgtggtaatc cagtggcatc acttcatcac
    23341 cacttaatga aagaggcaga ccaggccata aagagacatc ttcaggcagg tcgcaccagg
    23401 taatgttagg cattaaggat cctgtaaaat cgttcgaagc agggaaaata acgcatgagg
    23461 ctaccttgta tccattgctt ctggcaacat taagtctcaa attttcaaag ggtggaagat
    23521 ggctcgcaca aaactgaaat tccggctgca tcgggcagtg attgtcctgt tctgtcttgc
    23581 cttgttagtg gcgctgatgc agggagcgtc atggtttagt caaaaccacc agcgacaacg
    23641 taatccacag ctggaagaac tggcccgcac cctggcgcgt caggtaacgc tgaacgttgc
    23701 accgctgatg cgtaccgact caccggatga aaaacgcatt caggcgatcc tcgatcagtt
    23761 aacggatgaa agccgtatcc tcgacgcggg tgtgtatgac gaacaaggcg atcttatcgc
    23821 acgttctggc gaaagcgtcg aagtgcgcga ccggctggcg ctcgacggta aaaaagcggg
    23881 cggctatttt aaccagcaga ttgtcgaacc aattgccggg aaaaacggac cgctcggcta
    23941 tctgcgcctg acgctcgaca cccatacgct cgccaccgaa gcccaacagg tggataacac
    24001 cactaacatt ttacgcctga tgttgctgct ttcactggca atcggcgtag tgctgacccg
    24061 cacgctgcta cagggtaaac gtacccgctg gcagcaatcg cccttcctgt taaccgccag
    24121 caaaccggtg ccggaagagg aagaaagcga gaaaaaagag tgacccatta ctacaagaaa
    24181 ggaaatcgtt atgtccacat tacgcctgct catctctgac tcttacgacc cgtggtttaa
    24241 cctggcggtg gaggagtgta tttttcgcca gatgcccgcc acgcagcgcg ttctgtttct
    24301 ctggcgcaat gccgacacgg tagtgattgg tcgcgcgcag aacccgtgga aagagtgtaa
    24361 tacccggcgg atggaagaag ataacgtccg cctggcacgg cgcagtagcg gtggcggcgc
    24421 ggtgttccac gatctcggca atacctgctt tacctttatg gctggcaagc cggagtacga
    24481 taaaaccatc tccacgtcga ttgtgctcaa tgcgctgaac gcgcttggcg tcagcgccga
    24541 agcgtccggg cgtaacgatc tggtggtgaa aaccgccgaa ggcgaccgca aagtctcagg
    24601 atcggcctat cgcgaaacca aagatcgtgg cttccaccac ggcaccttgc tgctcaatgc
    24661 cgaccttagc cgcctggcaa actatctcaa tccggataaa aagaaactgg cggcgaaagg
    24721 cattacctca gtgcgttccc gcgtgaccaa cctcaccgag ctgctgccgg ggatcaccca
    24781 tgagcaggtt tgcgaggcca taaccaaggc ctttttcgcc cattatggcg agcgtgtaga
    24841 agcggaaatc atctccccgg acaaaacgcc agacttgcca aacttcgccg aaatctttgc
    24901 ccgtcagagt agctgggaat ggaacttcgg tcaggctccg gcattctcgc atctgctgga
    24961 tgaacgcttt agctggggcg gcgtggaact gcatttcgac gttgaaaaag gccatatcac
    25021 ccgcgcccag gtgtttaccg acagcctcaa ccccgcgccg ctggaagccc tcgccgggcg
    25081 actgcaaggc tgcctgtacc gcgcggatat gctgcaacaa gagtgcgaag cgctgttggt
    25141 tgacttcccg gaccaggaaa aagagctacg ggagttgtcg acgtggatag cgggggcggt
    25201 aaggtaactc gctgattaaa ataccaaagg aagggaatat gatcgatctg gaaaaccagg
    25261 agcgggagat tatcaattta atgttaagcc agcgtatttc ctggctagcc gccgtgcgta
    25321 tcaggcataa attatcgctt gctgaagtaa gtaaaatgtt aggtatctcg attaattctc
    25381 taaaatagat cgaaaagacg gaacggttta gctcaaacat caaaagcaaa atggcagaaa
    25441 tttatggttg tccaccagag ttgctgatat gcccttcctg gatgacggct gaacataaat
    25501 gaatagatt
//
LOCUS       DAJTMC010000043        25242 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.043, whole genome shotgun sequence.
ACCESSION   DAJTMC010000043 DAJTMC010000000
VERSION     DAJTMC010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25242
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.043"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(<1..403)
                     /locus_tag="OO130_001583"
     CDS             complement(<1..403)
                     /locus_tag="OO130_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HCR5365041.1"
                     /translation="MDTTLPFLILYACLSALLFFWDAKHGLLPDRFTCPLLWSGLLFY
                     QVCHPDGLADALWGTIVGYGTFAVIYWGYRILRHKEGLGYGDVKFLAALGAWHSWAFL
                     PRLVFLAASFACGAVVIGLLMRGKESLKNPLP"
     gene            782..1093
                     /gene="rpsJ"
                     /locus_tag="OO130_001584"
     CDS             782..1093
                     /gene="rpsJ"
                     /locus_tag="OO130_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HCR5365042.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            1126..1755
                     /gene="rplC"
                     /locus_tag="OO130_001585"
     CDS             1126..1755
                     /gene="rplC"
                     /locus_tag="OO130_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HCR5365043.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            1766..2371
                     /gene="rplD"
                     /locus_tag="OO130_001586"
     CDS             1766..2371
                     /gene="rplD"
                     /locus_tag="OO130_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HCR5365044.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            2368..2670
                     /gene="rplW"
                     /locus_tag="OO130_001587"
     CDS             2368..2670
                     /gene="rplW"
                     /locus_tag="OO130_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HCR5365045.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            2688..3509
                     /gene="rplB"
                     /locus_tag="OO130_001588"
     CDS             2688..3509
                     /gene="rplB"
                     /locus_tag="OO130_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HCR5365046.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            3526..3804
                     /gene="rpsS"
                     /locus_tag="OO130_001589"
     CDS             3526..3804
                     /gene="rpsS"
                     /locus_tag="OO130_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HCR5365047.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            3819..4151
                     /gene="rplV"
                     /locus_tag="OO130_001590"
     CDS             3819..4151
                     /gene="rplV"
                     /locus_tag="OO130_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HCR5365048.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            4169..4870
                     /gene="rpsC"
                     /locus_tag="OO130_001591"
     CDS             4169..4870
                     /gene="rpsC"
                     /locus_tag="OO130_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HCR5365049.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            4883..5293
                     /gene="rplP"
                     /locus_tag="OO130_001592"
     CDS             4883..5293
                     /gene="rplP"
                     /locus_tag="OO130_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HCR5365050.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            5293..5484
                     /gene="rpmC"
                     /locus_tag="OO130_001593"
     CDS             5293..5484
                     /gene="rpmC"
                     /locus_tag="OO130_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HCR5365051.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            5484..5738
                     /gene="rpsQ"
                     /locus_tag="OO130_001594"
     CDS             5484..5738
                     /gene="rpsQ"
                     /locus_tag="OO130_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HCR5365052.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            5903..6274
                     /gene="rplN"
                     /locus_tag="OO130_001595"
     CDS             5903..6274
                     /gene="rplN"
                     /locus_tag="OO130_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HCR5365053.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            6285..6599
                     /gene="rplX"
                     /locus_tag="OO130_001596"
     CDS             6285..6599
                     /gene="rplX"
                     /locus_tag="OO130_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HCR5365054.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            6614..7153
                     /gene="rplE"
                     /locus_tag="OO130_001597"
     CDS             6614..7153
                     /gene="rplE"
                     /locus_tag="OO130_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HCR5365055.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            7168..7473
                     /gene="rpsN"
                     /locus_tag="OO130_001598"
     CDS             7168..7473
                     /gene="rpsN"
                     /locus_tag="OO130_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HCR5365056.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            7507..7899
                     /gene="rpsH"
                     /locus_tag="OO130_001599"
     CDS             7507..7899
                     /gene="rpsH"
                     /locus_tag="OO130_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HCR5365057.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            7912..8445
                     /gene="rplF"
                     /locus_tag="OO130_001600"
     CDS             7912..8445
                     /gene="rplF"
                     /locus_tag="OO130_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HCR5365058.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRIKEAKKK"
     gene            8455..8808
                     /gene="rplR"
                     /locus_tag="OO130_001601"
     CDS             8455..8808
                     /gene="rplR"
                     /locus_tag="OO130_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HCR5365059.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            8823..9326
                     /gene="rpsE"
                     /locus_tag="OO130_001602"
     CDS             8823..9326
                     /gene="rpsE"
                     /locus_tag="OO130_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HCR5365060.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            9330..9509
                     /gene="rpmD"
                     /locus_tag="OO130_001603"
     CDS             9330..9509
                     /gene="rpmD"
                     /locus_tag="OO130_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HCR5365061.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            9513..9947
                     /gene="rplO"
                     /locus_tag="OO130_001604"
     CDS             9513..9947
                     /gene="rplO"
                     /locus_tag="OO130_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HCR5365062.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            9955..11286
                     /gene="secY"
                     /locus_tag="OO130_001605"
     CDS             9955..11286
                     /gene="secY"
                     /locus_tag="OO130_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HCR5365063.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            11318..11434
                     /gene="rpmJ"
                     /locus_tag="OO130_001606"
     CDS             11318..11434
                     /gene="rpmJ"
                     /locus_tag="OO130_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HCR5365064.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            11581..11937
                     /gene="rpsM"
                     /locus_tag="OO130_001607"
     CDS             11581..11937
                     /gene="rpsM"
                     /locus_tag="OO130_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HCR5365065.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            11954..12343
                     /gene="rpsK"
                     /locus_tag="OO130_001608"
     CDS             11954..12343
                     /gene="rpsK"
                     /locus_tag="OO130_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HCR5365066.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            12377..12997
                     /gene="rpsD"
                     /locus_tag="OO130_001609"
     CDS             12377..12997
                     /gene="rpsD"
                     /locus_tag="OO130_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HCR5365067.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            13023..14012
                     /gene="rpoA"
                     /locus_tag="OO130_001610"
     CDS             13023..14012
                     /gene="rpoA"
                     /locus_tag="OO130_001610"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HCR5365068.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            14053..14436
                     /gene="rplQ"
                     /locus_tag="OO130_001611"
     CDS             14053..14436
                     /gene="rplQ"
                     /locus_tag="OO130_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HCR5365069.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            14543..14911
                     /locus_tag="OO130_001612"
     CDS             14543..14911
                     /locus_tag="OO130_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HCR5365070.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILRGIRHDDPQYQEVSRRLSLLELKLRQAGF
                     STDFLRGDYADKLLDKINDN"
     gene            14922..15347
                     /gene="zntR"
                     /locus_tag="OO130_001613"
     CDS             14922..15347
                     /gene="zntR"
                     /locus_tag="OO130_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HCR5365071.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            15403..15621
                     /gene="arfA"
                     /locus_tag="OO130_001614"
     CDS             15403..15621
                     /gene="arfA"
                     /locus_tag="OO130_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HCR5365072.1"
                     /translation="MSRYQHTKGQIRDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGAC"
     gene            complement(15618..16028)
                     /gene="mscL"
                     /locus_tag="OO130_001615"
     CDS             complement(15618..16028)
                     /gene="mscL"
                     /locus_tag="OO130_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HCR5365073.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLAEIRDLLKEQNNRS"
     gene            complement(16158..17534)
                     /gene="trkA"
                     /locus_tag="OO130_001616"
     CDS             complement(16158..17534)
                     /gene="trkA"
                     /locus_tag="OO130_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HCR5365074.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(17556..18845)
                     /gene="rsmB"
                     /locus_tag="OO130_001617"
     CDS             complement(17556..18845)
                     /gene="rsmB"
                     /locus_tag="OO130_001617"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HCR5365075.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRQQEELLAKFNASDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRVNRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRHH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            complement(18891..19838)
                     /gene="fmt"
                     /locus_tag="OO130_001618"
     CDS             complement(18891..19838)
                     /gene="fmt"
                     /locus_tag="OO130_001618"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HCR5365076.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEEKGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERYIRAFNPWPMSWLEIERQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLA"
     gene            complement(19853..20362)
                     /gene="def"
                     /locus_tag="OO130_001619"
     CDS             complement(19853..20362)
                     /gene="def"
                     /locus_tag="OO130_001619"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCR5365077.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEADGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            20492..21616
                     /gene="dprA"
                     /locus_tag="OO130_001620"
     CDS             20492..21616
                     /gene="dprA"
                     /locus_tag="OO130_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HCR5365078.1"
                     /translation="MVDTDIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLLATTDYPGALFVEGEL
                     YALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLATSLLEHGGALVSEFPLDVSPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            21588..22061
                     /locus_tag="OO130_001621"
     CDS             21588..22061
                     /locus_tag="OO130_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HCR5365079.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFDREDIYNAL
                     LWLEKLADYQEGLTEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLEI
                     REMVIERVLALDTAEFDLEDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            22090..22632
                     /locus_tag="OO130_001622"
     CDS             22090..22632
                     /locus_tag="OO130_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HCR5365080.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPACGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            22637..23209
                     /gene="tsaC"
                     /locus_tag="OO130_001623"
     CDS             22637..23209
                     /gene="tsaC"
                     /locus_tag="OO130_001623"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HCR5365081.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVLGETGGRLNPSEIRDALTGELFRQG"
     gene            23214..24032
                     /gene="aroE"
                     /locus_tag="OO130_001624"
     CDS             23214..24032
                     /gene="aroE"
                     /locus_tag="OO130_001624"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HCR5365082.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFSAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLQGDNTD
                     GIGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHLGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            24029..24286
                     /locus_tag="OO130_001625"
     CDS             24029..24286
                     /locus_tag="OO130_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HCR5365083.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(24262..24816)
                     /locus_tag="OO130_001626"
     CDS             complement(24262..24816)
                     /locus_tag="OO130_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HCR5365084.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGGVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         6457 a         5996 c         6569 g         6220 t
ORIGIN      
        1 aaggcagcgg gttttttaat gattcttttc ctctcatgag cagaccaata actacggctc
       61 cgcaagcgaa tgaggcagca aggaaaacca gtcgtggtaa gaaggcccat gaatgccagg
      121 cgccaagggc ggcgagaaac ttcacatcac catagcctaa tccttcttta tgtcgcagta
      181 tgcgataccc ccagtaaatg acagcgaatg tgccgtaacc aacaattgtc ccccataacg
      241 catcggctaa accatcagga tgacaaactt gatagaacaa aagacctgac cagagtaacg
      301 ggcacgtaaa tctgtcaggt aacaagccat gctttgcatc ccagaaaaaa agtagggctg
      361 ataaacaagc gtacaaaatc agaaagggaa gggtagtgtc cataaaacat cctctgtaat
      421 tggagtatca ccatcgaata actccggatg ctcagcaaca gagagagcgc tgatttgtta
      481 acctgattcc ggtgaaaaac gctatcttca caaccttaca aatttcgtgg aatgcagctc
      541 gcaatctgcg cgttgcgatt aatttctacc cgatgcttgc gtcaggctca gatttgcgta
      601 taatgcgcgg gcttgtcgta gttgacagca ggttcaatct gaacctcagt tagccgaatt
      661 tggctaccta acaatgctcc caatcgggga gctacgtaag aacggttaca ctctcccatc
      721 aatcgtaatg ggtctgagga gtaatcattt tcgtttataa aataattgga gctctggtct
      781 catgcagaac caaagaatcc gtatccgcct gaaagcgttt gatcatcgtc tgatcgatca
      841 agcaaccgcg gaaatcgtcg agactgccaa gcgcactggt gcgcaggtcc gtggtccgat
      901 cccgctgccg acacgcaaag agcgcttcac tgttctgatc tccccgcacg tcaacaaaga
      961 cgcgcgcgat cagtacgaaa tccgtactca cttgcgtctg gttgacatcg ttgagccaac
     1021 cgagaaaacc gttgatgctc tgatgcgtct ggatctggct gccggtgtag acgtgcagat
     1081 cagcctgggt taatcaggtc attgagcgat tgagaggttg aaacaatgat tggtttagtc
     1141 ggtaaaaaag tgggtatgac ccgtatcttc acagaagacg gcgtttctat cccagtaacc
     1201 gtaatcgaag ttgaagcaaa ccgcgttact caggttaaag acctggctaa cgatggctac
     1261 cgtgctattc aggtgaccac cggtgctaaa aaagctaacc gtgtgaccaa gcctgaagct
     1321 ggccacttcg ctaaagctgg cgtagaagct ggccgtggtc tgtgggaatt ccgcctggct
     1381 gaaggcgaag agttcactgt aggtcagagc attagcgttg aactgtttgc tgacgttaaa
     1441 aaagttgacg taactggcac ctctaaaggt aaaggtttcg caggtaccgt taagcgctgg
     1501 aacttccgta cccaggacgc tactcacggt aactccttgt ctcaccgcgt tccgggttct
     1561 atcggtcaga accagactcc gggcaaagtg ttcaaaggca agaaaatggc aggtcagatg
     1621 ggtaacgaac gtgtaaccgt tcagagcctt gacgtagtac gcgttgacgc tgagcgcaac
     1681 ctgctgctgg ttaaaggtgc tgtcccgggt gcaaccggta gcgacctgat cgttaaacca
     1741 gctgtgaagg cgtaaggaga tagcaatgga attagtattg aaagacgcgc agagcgcgct
     1801 gactgtttcc gaaactacct tcggtcgtga tttcaacgaa gcgctggttc accaggttgt
     1861 tgttgcttat gcagctggtg ctcgtcaggg tactcgtgct cagaagactc gtgctgaagt
     1921 aactggttcc ggtaaaaaac cgtggcgcca gaaaggcacc ggccgtgcgc gttctggttc
     1981 tatcaagagc ccgatctggc gttctggtgg cgtgaccttt gctgctcgtc cgcaggacca
     2041 cagtcaaaaa gttaacaaga agatgtaccg cggcgcgctg aaaagcatcc tgtccgaact
     2101 ggtacgtcag gatcgtctga tcgttgtcga gaagttctct gtagaagcgc cgaaaactaa
     2161 gctgctggca cagaaactga aagacatggc tctggaagat gtgctgatca tcaccggtga
     2221 gctggacgaa aacctgttcc tggctgcgcg caacctgcac aaggttgacg tacgcgatgc
     2281 aactggtatc gacccggtta gcctgatcgc cttcgacaaa gtcgtaatga ctgctgatgc
     2341 tgttaagcaa gttgaggaga tgctggcatg attcgtgaag aacgtctgct gaaggtgctg
     2401 cgtgcaccgc acgtttctga aaaagcgtct actgcgatgg aaaaatccaa caccatcgta
     2461 ctcaaagttg ctaaagacgc gaccaaagca gaaatcaaag ctgctgtgca gaaactgttt
     2521 gaagtcgaag tcgaagtcgt taacaccctg gtagttaaag ggaaagttaa acgtcacgga
     2581 cagcgtatcg gtcgtcgtag cgactggaaa aaagcttacg tcaccctgaa agaaggccag
     2641 aatctggact tcgttggcgg cgctgagtaa gtcggaggag taatacaatg gcagttgtta
     2701 aatgtaaacc gacatctccg ggtcgtcgcc acgtagttaa agtggttaac cctgagctgc
     2761 acaagggcaa accttttgct ccgttgctgg aaaaaaacag caaatccggt ggtcgtaaca
     2821 acaatggccg tatcaccact cgtcatatcg gtggtggcca caagcaggct taccgtattg
     2881 ttgacttcaa acgcaacaaa gacggtatcc cggcagttgt tgaacgtctt gagtacgatc
     2941 cgaaccgttc cgcgaacatc gcgctggttc tgtacaaaga cggtgaacgc cgttacatcc
     3001 tggcccctaa aggcctgaaa gctggcgacc agattcagtc tggcgttgat gctgcaatca
     3061 aaccaggtaa caccctgccg atgcgcaaca tcccggttgg ttctactgtt cataacgtag
     3121 aaatgaaacc aggtaaaggc ggtcagctgg cacgttccgc tggtacttac gttcagatcg
     3181 ttgctcgtga tggtgcttat gtcaccctgc gtctgcgttc tggtgaaatg cgtaaagtag
     3241 aagcagactg ccgtgcaact ctgggcgaag ttggcaatgc tgagcatatg ctgcgcgttc
     3301 tgggtaaagc aggtgctgca cgctggcgtg gtgttcgtcc gaccgttcgc ggtaccgcga
     3361 tgaacccggt agaccaccca catggtggtg gtgaaggtcg taactttggt aagcacccgg
     3421 taactccgtg gggcgttcag accaaaggta agaagacccg cagcaacaag cgtactgata
     3481 aattcatcgt acgtcgccgt agcaaataat tttagaggat aagccatgcc acgttctctc
     3541 aagaaaggtc cttttattga cctgcacttg ctgaagaagg tagagaaagc ggtggaaagc
     3601 ggagacaaga agcccctgcg cacttggtcc cgtcgttcaa cgatctttcc taacatgatc
     3661 ggtttgacca tcgctgtcca taatggtcgt cagcacgttc cggtatttgt aaccgacgaa
     3721 atggtcggtc acaaactggg tgaattcgca ccgactcgta cttatcgcgg ccacgctgct
     3781 gataaaaaag cgaagaagaa ataaggtagg aggaagagat ggaaactatc gctaaacatc
     3841 gccatgctcg ttcttctgct cagaaggttc gccttgttgc tgacctgatt cgcggtaaga
     3901 aagtgtcgca ggctctggat attttgacct acaccaacaa gaaagcggct gtactggtca
     3961 agaaagttct ggaatctgcc attgctaacg ctgaacacaa cgatggcgct gacattgacg
     4021 atctgaaagt tacgaaaatt ttcgtagacg aaggcccgag catgaagcgc attatgccgc
     4081 gtgcaaaagg tcgtgcagat cgcatcctga agcgcaccag ccacatcact gtggttgtgt
     4141 ccgatcgctg agactctgga gactagcaat gggtcagaaa gtacatccta atggtattcg
     4201 cctgggtatt gtaaaaccat ggaactctac ctggtttgcg aacaccaaag aattcgctga
     4261 caacctggac agcgatttta aagtacgtca gtacctgact aaggaactgg ctaaagcgtc
     4321 cgtatctcgt atcgttatcg agcgtccggc taagagcatc cgtgtaacca ttcacactgc
     4381 tcgcccgggt atcgttatcg gtaaaaaagg tgaagacgta gaaaaactgc gtaaggtcgt
     4441 agcggacatc gctggcgttc ctgcacagat caacatcgcc gaagttcgta agcctgaact
     4501 ggacgcaaaa ctggttgctg acagcatcac ttctcagctg gaacgtcgcg ttatgttccg
     4561 tcgtgctatg aagcgtgctg tacagaacgc aatgcgtctg ggcgctaaag gtattaaagt
     4621 tgaagttagc ggccgtctgg gcggcgcgga aatcgcacgt accgaatggt accgcgaagg
     4681 tcgcgtaccg ctgcacactc tgcgtgctga catcgactac aacacctctg aagcgcacac
     4741 cacttacggt gtaatcggcg ttaaagtgtg gatcttcaaa ggcgagatcc tgggtggtat
     4801 ggctgctgtt gaacaaccgg aaaaaccggc tgctcagcct aaaaagcagc agcgtaaagg
     4861 ccgtaaataa ggagcgtcgc tgatgttaca accaaagcgt acaaaattcc gtaaaatgca
     4921 caaaggccgt aaccgcggtc tggcgcaggg tacggatgtt agcttcggca gcttcggtct
     4981 gaaagctgtt ggccgtggtc gtctgactgc ccgtcagatc gaagcagcac gtcgtgctat
     5041 gacccgtgca gttaagcgtc aaggtaagat ctggatccgt gtgttcccgg acaaaccgat
     5101 cactgaaaag ccgctggcag tgcgtatggg taaaggtaaa ggtaacgtgg agtattgggt
     5161 tgccttgatt cagccgggta aagtcctgta tgaaatggac ggtgttccgg aagagctggc
     5221 ccgtgaagca ttcaagctgg cagcagcgaa actgccgatt aaaaccacct ttgtaactaa
     5281 gacggtgatg taatgaaagc aaaagagctg cgtgagaaga gcgttgaaga gctgaacacc
     5341 gagctgctga acctgctgcg tgagcagttc aacctgcgta tgcaggctgc aagtggccag
     5401 ctgcaacagt ctcacctgtt gaagcaagtg cgtcgcgatg tcgcacgcgt taagacttta
     5461 ctgaacgaga aggctggtgc gtaatgaccg ataaaatccg tactctgcaa ggtcgcgttg
     5521 ttagcgacaa aatggagaaa tccattgttg ttgctatcga acgttttgtg aaacacccga
     5581 tctacggtaa attcatcaag cgtacgacca aactgcacgt acatgacgag aacaacgaat
     5641 gcggtatcgg tgacgtggtt gaaatccgcg aatgccgtcc gctgtccaag actaaatcct
     5701 ggacgctggt tcgcgttgta gagaaagcgg ttctgtaata cagtacactc tctcgatacg
     5761 aataaacggc tcagaaatga gccgtttatt ttttctaccc atatccttga agcggtgtta
     5821 taatgccgcg ccctcgatat ggggattttt aacgacctga ttttcgggtc tcagtagtag
     5881 ttgacattag cggagcacta aaatgatcca agaacagact atgctgaacg tcgccgacaa
     5941 ctccggtgca cgtcgcgtaa tgtgtatcaa ggttctgggt ggctcgcacc gtcgctacgc
     6001 aggcgtaggc gacatcatca agatcaccat caaagaagca attccgcgtg gtaaggtcaa
     6061 aaaaggtgat gtgctgaagg cggtagtggt gcgcaccaag aagggtgttc gtcgcccgga
     6121 cggttctgtc attcgcttcg atggtaatgc ttgtgttctt ctgaacaaca acagcgagca
     6181 gcctatcggt acgcgtattt ttgggccggt aactcgtgag cttcgtagtg agaagttcat
     6241 gaaaattatc tctctggcac cagaagtact ctaaggagcg aatcatggca gcgaaaatcc
     6301 gtcgtgatga cgaagttatc gtgttaaccg gtaaagataa aggtaaacgc ggtaaagtta
     6361 agaatgtcct gtcttccggc aaggtcattg ttgaaggtat caacctggtt aagaaacatc
     6421 agaagccggt tccggccctg aaccaaccgg gtggcatcgt tgaaaaagaa gccgctattc
     6481 aggtttccaa cgtagcaatc ttcaatgcgg caaccggcaa ggctgaccgt gtaggcttta
     6541 gattcgaaga cggcaaaaaa gtccgtttct tcaagtctaa cagcgaaact atcaagtaat
     6601 ttggagtagt acgatggcga aactgcatga ttactacaaa gacgaagtag ttaaaaaact
     6661 catgactgag tttaactaca attctgtcat gcaagtccct cgggtcgaga agatcaccct
     6721 gaacatgggt gttggtgaag cgatcgctga caaaaaactg ctggataacg cagcagcaga
     6781 cctggcagca atctccggtc aaaaaccgct gatcaccaaa gcacgcaaat ctgttgcagg
     6841 cttcaaaatc cgtcagggct atccgatcgg ctgtaaagta actctgcgtg gcgaacgcat
     6901 gtgggagttc tttgagcgcc tgatcactat tgctgtacct cgtatccgtg acttccgtgg
     6961 cctgtccgct aagtctttcg acggtcgtgg taactacagc atgggtgtcc gtgagcagat
     7021 catcttccca gaaatcgact acgataaagt cgaccgcgtt cgtggtttgg atattaccat
     7081 taccactact gcgaaatctg acgaagaagg ccgcgctctg ctggctgcct ttgacttccc
     7141 gttccgcaag taaggtaggg ttactaaatg gctaagcaat caatgaaagc acgcgaagta
     7201 aaacgcgtag ctttagctga taaatacttc gcgaaacgcg ctgaactgaa agcgatcatc
     7261 tctgatgtga acgcttccga cgaagatcgt tggaacgctg ttctcaagct gcagactctg
     7321 ccgcgtgatt ccagcccgtc tcgtcagcgt aaccgctgcc gtcaaacagg tcgtccgcat
     7381 ggtttcctgc ggaagttcgg gttgagccgt attaaggtcc gtgaagccgc tatgcgcggt
     7441 gaaatcccgg gtctgaaaaa ggctagctgg taattgtcac caattgaatc acgggagtaa
     7501 agacagatga gcatgcaaga tccgatcgcg gatatgctga cccgtatccg taacggtcag
     7561 gccgcgaaca aagctgcggt caccatgcct tcctccaagc tgaaagtggc aatcgccaac
     7621 gtgctgaagg aagaaggttt tattgaagat tttaaagttg aaggcgacac caagcctgaa
     7681 ctggaactta ctctgaagta tttccagggc aaagctgttg tagaaagcat tcagcgtgtc
     7741 agccgcccag gtctgcgcat ctataaacgt aaagatgagc tgccgaaagt tatggcgggt
     7801 ctgggtatcg cagttgtttc tacctctaaa ggtgttatga ctgatcgtgc agcgcgccag
     7861 gctggtcttg gtggcgaaat tatctgctac gtagcctaat cggaggaaaa aatgtctcgt
     7921 gttgctaaag caccggtcgt tgttcctgcc ggcgttgacg taaaaatcaa cggtcaggtt
     7981 attacgatca aaggtaaaaa cggcgagctg actcgtactc tcaacgatgc tgttgaagtt
     8041 aaacatgcag ataataccct gaccttcggt ccgcgtgatg gttacgcaga cggttgggca
     8101 caggctggta ccgcgcgtgc cctgctgaac tcaatggtta tcggtgttac cgaaggcttc
     8161 actaagaagc tgcagctggt tggtgtaggt taccgtgcag cggttaaagg taatgtgatt
     8221 aacctgtctc tgggtttctc tcatcctgtt gaccatcagc tgcctgcggg tatcactgct
     8281 gaatgtccga ctcagactga aatcgtgctg aaaggcgctg ataagcaggt gatcggccag
     8341 gttgcagcgg atctgcgcgc ctaccgtcgt cctgagcctt ataaaggcaa gggtgttcgt
     8401 tacgccgacg aagtcgtgcg tatcaaagag gctaagaaga agtaaggtaa cactatggat
     8461 aagaaatctg ctcgtatccg tcgtgcgacc cgcgcacgcc gcaagctcca ggagctgggc
     8521 gcaactcgcc tggtggtaca tcgtaccccg cgtcacattt acgcacaggt aattgcaccg
     8581 aacggttctg aagttctggt agctgcttct actgtagaaa aagctatcgc tgaacaactg
     8641 aagtacaccg gtaacaaaga cgcggctgca gctgtgggta aagctgtcgc tgaacgcgct
     8701 ctggaaaaag gcatcaaaga tgtatccttt gaccgttccg ggttccaata tcatggtcgt
     8761 gtccaggcac tggcagatgc tgcccgtgaa gctggccttc agttctaagg tagaggtgta
     8821 agatggctca catcgaaaaa caagctggcg aactgcagga aaagctgatc gcggtaaacc
     8881 gcgtatctaa aaccgttaaa ggtggtcgta ttttctcctt cacagctctg actgtagttg
     8941 gcgatggtaa cggtcgcgtt ggttttggtt acggtaaagc gcgtgaagtt ccagcagcga
     9001 tccagaaagc gatggaaaaa gcccgtcgca atatgattaa cgtcgcgctg aataacggca
     9061 ctctgcaaca ccctgttaaa ggtgttcaca cgggttctcg cgtattcatg cagccggctt
     9121 ccgaaggtac cggtatcatc gccggtggtg caatgcgcgc cgttctggaa gtcgctgggg
     9181 ttcataacgt tctggctaaa gcatatggtt ccaccaaccc gatcaacgtg gttcgtgcaa
     9241 ctattgatgg cctggaaaat atgaactctc cagaaatggt cgctgccaag cgtggtaaat
     9301 ccgttgaaga aattctgggg aaataaacca tggcaaagac tattaaaatt actcaaaccc
     9361 gcagtgcaat cggtcgtctg ccgaaacaca aggcaacgct gcttggcctg ggtctgcgtc
     9421 gtattggtca caccgtagag cgcgaggata ctcctgctat tcgcggtatg atcaacgcgg
     9481 tttccttcat ggttaaagtt gaggagtaag agatgcgttt aaatactctg tctccggccg
     9541 aaggctccaa aaaggcgggt aaacgcttgg gtcgtggtat cggttctggc ctcggtaaaa
     9601 ccggtggtcg tggtcacaaa ggtcagaagt ctcgttctgg cggtggcgta cgtcgcggtt
     9661 tcgagggtgg tcagatgcct ctgtaccgtc gtctgccgaa attcggcttc acttctcgta
     9721 aagcagcgat tacagccgaa gttcgtctgt ctgacctggc taaagtagaa ggcggtgttg
     9781 tagacctgaa cacgctgaaa gcggctaaca ttatcggtat ccagatcgag ttcgcgaaag
     9841 tgatcctggc tggcgaagta acgactccgg taactgttcg tggcctgcgt gttactaaag
     9901 gcgctcgtgc tgctatcgaa gctgctggcg gtaaaatcga ggaataagta gcagatggct
     9961 aaacaaccgg gattagattt tcaaagtgcc aaaggtggct taggcgagct gaaacgcaga
    10021 ctgctgtttg ttatcggtgc gctgattgtg ttccgtattg gctcttttat tccgatccct
    10081 ggtattgatg ccgctgtact tgccaaactg cttgagcaac agcgaggcac catcattgag
    10141 atgtttaaca tgttctctgg tggtgctctc agccgtgctt ctatctttgc tctggggatc
    10201 atgccgtata tttcggcgtc gatcattatc cagctgctga cggtggttca cccaacgttg
    10261 gcggaaatta agaaagaagg ggagtctggt cgtcgtaaga tcagccagta cacccgctac
    10321 ggtactctgg tgctggcaat attccagtcg atcggtattg ctaccggtct gccgaatatg
    10381 cctggtatgc aaggcctggt gattaacccg ggctttgcat tctacttcac cgctgttgta
    10441 agtctggtca caggaacgat gttcctgatg tggttgggcg aacagatcac tgaacgaggt
    10501 atcggcaacg gtatttcaat cattatcttc gccggtattg tcgcgggact cccgccagcc
    10561 attgcccata ctatcgagca agcgcgtcaa ggcgacctgc acttcctcgt gttgctgttg
    10621 gttgcagtat tagtatttgc agtgacgttc tttgttgtat ttgttgagcg tggtcaacgc
    10681 cgcattgtgg taaactacgc gaaacgtcag caaggtcgtc gtgtctatgc tgcacagagc
    10741 acacatttac cgctgaaagt gaatatggcg ggggtaatcc cggcaatctt cgcttccagt
    10801 attattctgt tcccggcgac catcgcgtca tggttcgggg gcggtactgg ttggaactgg
    10861 ctgacaacaa tttcgctgta tttgcagcct gggcaaccgc tttatgtgtt actctatgcg
    10921 tctgcaatca tcttcttctg tttcttctac acggcgttgg ttttcaaccc gcgtgaaaca
    10981 gcagataacc tgaagaagtc cggtgcattt gtaccaggaa ttcgtccggg agagcaaacg
    11041 gcgaagtata tcgataaagt aatgacccgc ctgaccctgg ttggtgcgct gtatattacc
    11101 tttatctgcc tgatcccgga gttcatgcgt gatgcaatga aagtaccgtt ctacttcggt
    11161 gggacctcac tgcttatcgt tgttgtcgtg attatggact ttatggctca agtgcaaact
    11221 ctgatgatgt ccagtcagta tgagtctgca ttgaagaagg cgaacctgaa aggctacggc
    11281 cgataattgg tcgcccgaga agttacggag agtaaaaatg aaagttcgtg cttccgtcaa
    11341 gaaattatgc cgtaactgca aaatcgttaa gcgtgatggt gtcatccgtg tgatttgcag
    11401 tgccgagccg aagcataaac agcgccaagg ctgatttttt cgcatatttt tcttgcaaag
    11461 ttgggttgag ctggctagat tagccagcca atcttttgta tgtctgtgcg tttccatttg
    11521 agtatcctga aaacgggctt ttcagcatgg aacgtacata ttaaatagta ggagtgcata
    11581 gtggcccgta tagcaggcat taacattcct gatcataagc atgccgtaat cgcattaact
    11641 tcgatttatg gcgtcggcaa gacccgttct aaagccatcc tggctgcagc gggtatcgct
    11701 gaagatgtta agatcagtga gctgtctgaa ggacaaatcg acacgctgcg tgacgaagtt
    11761 gccaaatttg tcgttgaagg tgatctgcgc cgtgaaatca gcatgagcat caagcgcctg
    11821 atggatcttg gttgctatcg cggcttgcgt catcgtcgtg gtctcccggt tcgcggtcag
    11881 cgtaccaaga ccaacgcacg tacccgtaag ggtccgcgca aaccgatcaa gaaataatcg
    11941 gggtgattga ataatggcaa aggcaccaat tcgtgcacgt aaacgtgtaa gaaaacaagt
    12001 ctctgacggc gtggctcata tccatgcttc tttcaacaac accatcgtga ctatcactga
    12061 tcgtcagggt aacgcgttgg gttgggcaac agccggtggt tccggtttcc gtggttctcg
    12121 caaatccact ccgtttgcag ctcaggttgc agcagagcgt tgcgctgacg ccgtgaaaga
    12181 atacggcatc aagaatctgg aagttatggt taaaggtccg ggtccaggcc gcgaatctac
    12241 tattcgtgct ctgaacgccg caggtttccg catcactaac attactgatg tgactccgat
    12301 ccctcataac ggttgtcgtc cgccgaaaaa acgtcgcgta taacgcctcg ttttccaggt
    12361 ttgttggaga aagaaaatgg caagatattt gggtcctaag ctcaagctga gccgtcgtga
    12421 gggcaccgac ttattcctta agtctggcgt tcgcgcgatc gataccaagt gtaaaattga
    12481 acaagctcct ggccagcacg gtgcgcgtaa accgcgtctg tctgactatg gtgtgcagtt
    12541 gcgtgaaaag caaaaagttc gccgtatcta tggtgtgctg gagcgtcagt tccgtaacta
    12601 ctacaaagaa gcagcacgtc tgaaaggcaa caccggtgaa aacctgttgg ctctgctgga
    12661 aggtcgtctg gacaacgttg tataccgtat gggcttcggt gccactcgtg cagaagcacg
    12721 tcagctggtt agccataaag caattatggt aaacggtcgt gttgttaata tcgcttctta
    12781 tcaggttagt ccgaatgacg ttgtaagcat tcgtgagaaa gcgaagaagc agtctcgcgt
    12841 gaaagccgct ctggagctgg ctgagcagcg tgaaaagcca acttggctgg aagttgatgc
    12901 tggcaagatg gaaggtacgt ttaagcgtaa gccggagcgt tctgatctgt ctgcggacat
    12961 taacgaacac ctgatcgtcg agctttactc caagtaaagc ttagtaccaa agagaggaca
    13021 caatgcaggg ttctgtgaca gagtttctaa aaccgcgcct ggttgatatc gagcaagtga
    13081 gttcgacgca cgccaaggtg acccttgagc ctttagagcg tggctttggc catactctgg
    13141 gtaacgcact gcgccgtatt ctgctctcat cgatgccggg ttgcgcggtg accgaggttg
    13201 agattgatgg tgtactacat gagtacagca ccaaagaagg cgttcaggaa gatatcctgg
    13261 aaatcctgct caacctgaaa gggctggcgg tgagagttca gggcaaagat gaagttattc
    13321 ttaccttgaa taaatctggc attggccctg tgactgcagc cgatatcacc cacgacggtg
    13381 atgtcgaaat cgtcaagccg cagcacgtga tctgccacct gaccgatgag aacgcgtcta
    13441 ttagcatgcg tatcaaagtt cagcgcggtc gtggttatgt gccggcttct acccgaattc
    13501 attcggaaga agatgagcgc ccaatcggcc gtctgctggt cgacgcatgc tacagccctg
    13561 tggagcgtat tgcctacaat gttgaagcag cgcgtgtaga acagcgtacc gacctggaca
    13621 agctggtcat cgaaatggaa accaacggca caatcgatcc tgaagaggcg attcgtcgtg
    13681 cggcaaccat tctggctgaa caactggaag ctttcgttga cttacgtgat gtacgtcagc
    13741 ctgaagtgaa agaagagaaa ccagagttcg atccgatcct gctgcgccct gttgacgatc
    13801 tggaattgac tgtccgctct gctaactgcc ttaaagcaga agctatccac tatatcggtg
    13861 atctggtaca acgtaccgag gttgagctcc ttaaaacgcc taaccttggt aaaaaatctc
    13921 ttactgagat taaagacgtg ctggcttccc gtggactgtc tctgggcatg cgcctggaaa
    13981 actggccacc ggcaagcatc gctgacgagt aaccggatca caggttaagg ttttactgag
    14041 aaggataagg tcatgcgcca tcgtaagagt ggtcgtcaac tgaaccgcaa cagcagccat
    14101 cgccaggcta tgttccgcaa tatggcaggt tcactggttc gtcatgaaat catcaagacg
    14161 actctgccta aagcgaaaga gctgcgccgc gtagttgagc cgctgattac tcttgccaag
    14221 actgatagcg ttgctaatcg tcgtctggca ttcgcccgta ctcgtgataa cgagatcgtg
    14281 gcaaaactgt ttaacgaact gggcccgcgt ttcgcgagcc gtgccggtgg ttacactcgt
    14341 attctgaagt gtggcttccg tgcaggcgac aacgcgccga tggcttacat cgagctggtt
    14401 gatcgttcag agaaagcaga agctgctgca gagtaatctg aagcaacgta aaaaaacccg
    14461 ccccggcggg tttttttata cccgtagcat ccccacttat ctacaatagc tgtactcttt
    14521 ttgttcatcc cctggagtat ttatgtggtt acttgaccag tgggcagagc gtcatatagc
    14581 agaagcgcaa gcgaaaggtg agtttgataa tctggcaggt agcggcgaac cattgatact
    14641 ggatgatgat tctcatgtgc caccggaatt acgtgcgggg tatcgcttgc tgaagaatgc
    14701 tggttgctta ccgccagaac ttgagcagcg gagggaagca attcagcttc tggatatcct
    14761 gagaggtatc cgtcacgatg atccgcaata tcaagaggtt agccgtcgat tgtcattact
    14821 ggaattgaag ctgcgacaag ctggatttag taccgatttt ttacgcggcg attatgctga
    14881 caagttgttg gacaaaatca acgataacta gtggagtatg tatgtatcgc attggtgagc
    14941 tggcaaaaat ggcggaagta acacctgaca cgattcgtta ttacgaaaaa cagcagatga
    15001 tggagcatga agtgcgtact gaaggcgggt ttcgcctgta taccgaaagc gatctccagc
    15061 gattgaaatt tatccgccat gccagacaac taggtttcag tctggagtcg atccgcgagt
    15121 tgctgtcgat ccgcatcgat cctgaacacc atacctgtca ggagtcaaaa ggcattgtgc
    15181 aggaaagatt gcaggaagtc gaagcacgga tagccgagtt gcagagtatg cagcgttcct
    15241 tgcaacgcct taacgatgcc tgttgtggga ccgcccatag cagtgtttat tgttcgattc
    15301 ttgaagctct tgaacaaggg gcgagtggcg ttaagagtgg ctgttgattt tttgcactgg
    15361 caggattaca ctcgcgccgt aaaataacca attggagttt ttatgagccg atatcagcat
    15421 actaaagggc agataaggga taatgcgata gaagcattac tacatgatcc cttattccga
    15481 cagcgcgtag agaaaaataa gaaggggaaa ggcagttaca tgcgaaaagg aaaacatggc
    15541 aatcggggga actgggaggc cagtggcaag aaagtaaatc acttttttac cactggtctt
    15601 ctgctttcag gcgcttgtta agagcggtta ttctgctctt tcagcaaatc acgaatttct
    15661 gccagtaata cttcttcttt agttggtgca ggtgcggcag ctggttcttc ttttttccga
    15721 ttcagtttgt tgattagctt aatcgccata aagatggcaa aggccacaat cagaaaatca
    15781 aagacgtttt gaatgaagac accgtaatgc atcacaacag cagggatatc cccctgcgca
    15841 tcgcgtagcg tgacagcaaa ctgtttaaag tcgatcccgc caattaataa gcccagaggt
    15901 ggcatgatga tatcggcaac cagtgaagat acaatcttcc cgaatgccgc accgataatg
    15961 acacccaccg ccaaatccac cacgttcccg cgcatcgcaa attcgcgaaa ttctttaata
    16021 atgctcatgt tattctccct atgaagccga caaccataag tctaacaaat gttaagccat
    16081 tttcctgcca ggaatgaatt taattaatcc ttgataatca aagggaaata ataaggcgtc
    16141 attagacgcc ttattaatta caagaagaaa gggcttggct ggaagagtct ttcgacgtcg
    16201 gtaataaact ttttatctgt gaggaacata attacgtgat cgccttgctc aatgcgcaga
    16261 ttgtcattgg caatcatcac gtcgtttcca cgtaccaccg ctccaataat cgttcctggc
    16321 ggtagcttga tttcgtcaat gactctgccg acaacgcgtg aggtgctttc atcaccgtga
    16381 gcaacggctt caatagcttc tgctacgccg cggcgcaatg aggaaacacc aacaatatct
    16441 gcttttcgca catggctaag caacgcagaa atagttgctt gttgtggtga aattgcaata
    16501 tcgatcacgc tcccctgaac cagatccaca taagcgcgac gctggatcaa taccatcacc
    16561 tttttcgcac ccatacgttt ggcaagcatg gcggacatga tattggcctc gtcatcgttg
    16621 gtgacggcaa taaacagatc aacttgatcg atatgttctt cggccagcag ttcttgatcc
    16681 gacgcatcac caaaaaagac gatcgtattc tgcaactttt ccgccagttc ggcagcgcgc
    16741 tgctgattac gttcgatgag tttaacgctg taatcttttt ccagacgacg cgccagccct
    16801 gcaccgatat taccgccacc aaccagcatg atccgcttat acggtttttc cagtcgctgt
    16861 aattcactca tcaccgcgcg gatatgctgt gaagcggcaa taaagaacac ttcatcacca
    16921 gcttcaacaa tggtcgaacc ttgtggacga atggggcgat cgtggcggaa aattgctgcc
    16981 acacgagtat cgatatgtgg catatgttcg cgcatggtcg aaagtgcatt accaatcagc
    17041 gggccgccat aataggcttt aactacagcg aggctgactt taccctcagc gaagttcacc
    17101 acctgcaatg cgcctggata ctcaatcagt cggtaaatat tatcgataac caactgctct
    17161 ggtgcgatca gatgatcaat cggtacagca tctgaatgaa atagcttatc ggcatcgcgt
    17221 acgtagtctg gtgagcggat gcgagcgatg cgattagggg tgttgaaaag tgagtaggct
    17281 acctggcagg caaccatatt ggtttcatct gaactggtta cagcaaccag catatcggcg
    17341 tcgtcggcac ctgcctcccg caatacgcgt ggatgagagc catgcccctg cacgactcgc
    17401 aggtcaaatt tatcctgcaa ggtccgcaga cgctcaccgt tggtatcgac aacagtaata
    17461 tcgttgttct cgccaaccag gttttccgcc agtgtgccgc caacctggcc ggcacccaga
    17521 atgataattt tcatcagtct cgacccgtta tctcatcact ttttgattag cttagcgtaa
    17581 aagaagccgt cgccctcttc ggcaccaggc agattttgtt tacccggttg ctctggtgtt
    17641 cctgtttcgc aaagttcggc atcagcggta cgttgcaaaa aagctttaat ctgcaggcta
    17701 ttctcttccg gtaacaccga acaggtggca tagaccaggg ttccaccgga ttttaaatgc
    17761 ggccaaatgg cgtcgagaat ttcagactgc aattgcgcga gttccgggat atcgcgatcg
    17821 cgacgtaacc atttaatatc cggatggtga cgaatcaccc ccgttgctga acaaggcgca
    17881 tctaataaaa tgcgatcaaa ctgttgctcg ccacaccatt gggaagggta acggccatca
    17941 ccttgtttca cggttgcctt cataccaagg cgttttaaat tgtcgtaaac gcgagagagg
    18001 cgctgttcgt caatatcaac cgcaacaacc tgcgcttctg gtgccacctc aaggatatgc
    18061 gttgttttac cgccgggggc ggcacaaaga tccagaatgt gttcaccgtt ttgtggcgca
    18121 agccaggtca tgcaaccttg tgctgatgca tcctgaacgg taacccatcc gtcttcaaaa
    18181 ccaggtagcg catgaacagg tgcaggtgtt tccagacgta cagcatcagg gtaatccgca
    18241 tgcgggaaac ctttcattcc tgcttcatcc agcaatgcaa gccagctgtc gcgggaatga
    18301 tgcgtacgat taacgcgcag ccacattggc ggacgctggt tattggcttc gacgatggat
    18361 tgccactgct ctggatacgc tttttgcaga cgcttcagca accaggaagg atgcagataa
    18421 cgtgcatcac tggcattaaa cttggctaat aactcttctt gctgacgctg gaactggcgt
    18481 aatacaccgt taatcaaccc tttaagttgc ggacgcttaa ttgcgatagc gccttcaacc
    18541 gtttcagcca gcgcagcatg aggtggaatg cgggtataaa gcagttgata caaaccaacc
    18601 ataatcaggt aatgcacagt gcgctgtttg ccggtcatcg gacgggccat taacttatta
    18661 atcagccagt ctaactgcga aagcgtacgc agtacgccaa agcacaactc ttgaagaagt
    18721 gctttgtctt tatcagaaac tttttgctgg agcggtggca gaatgttgct taatgattgc
    18781 ccttgctcga cgacttgttc aacggcctgg gccgccatgc tacgtaaatt acgttgtttt
    18841 ttcataagta taaaaatgcc cggtaagacc gggcttagaa gagtggacta tcaggccaga
    18901 cggttgcccg gaacaaacca ttcccgacga gaattcagga ggtcttgcgc gctcatcgct
    18961 ttcttacccg caggttgtaa cgagagcaga ttcaggattc catcaccagt tgcaacctga
    19021 atgccttgtt tgttggcttc aaggatcgtt cctggtgcag cgttggttgc cgtatcaatg
    19081 accgacgctt tccagacttt aaccggctgt ctttcaattt ccagccagct cattggccat
    19141 ggattgaaag cgcgaatgta gcgttcaagc tgtgctgccg aaagtgacca gtcaatacgc
    19201 gcttcttctt tactcaactt ttcggcgtaa gtgacaagag tttcgtcctg aacttctggt
    19261 ttcgccgtgc cgtctgccag ttgtttcaac gtggtgataa gcccttgtgg gccaagctct
    19321 gccagcttgt cgtacagcgt accactggta tcttctgcag taatcgggca ggagagctta
    19381 tagagcatgt caccggtgtc taaaccgaca tccatttgca taatggtcac accagtttct
    19441 gcatcacccg cccatagtga gcgttggatt ggtgcagcac cgcgccagcg tggcagcagt
    19501 gaaccatgaa cgttgataca gccaagacgc ggcatctcca gcactgcttt aggcagaatt
    19561 aagccatagg cgacgacgac cataacatca gcctgcagat cggcgaccag ttgctggttt
    19621 tcttgtggac gcagggaaac aggttgaaaa acgggcagac ctttttcctc agccagaact
    19681 ttaaccgggc tgggcatcag ttttttaccg cgtcctgccg gtcggtctgg ctgggtgaac
    19741 acgccaacga cgttatgacc agaagacaac agcgcgtcga gatgacgcgc tgcaaagtca
    19801 ggtgtacccg caaaaataat acgtagtgat tctgacacgt tagttcttat ccttaagccc
    19861 gggctttcag acgatccagt ttttcaactt tctgacgaat acgttgttgt ttcaacggtg
    19921 acagataatc cataaacagt ttgccgacca ggtgatccat ctcatgctga atacagatgg
    19981 ctaacagacc gtctgcttcc aattcaaatg gtttaccgtc gcggtccagg gcgcgaattt
    20041 taactttctc tgcgcgtggc actaaagcac gttgttcagg gatcgacagg caaccttctt
    20101 caatgcctgt ttcgccgctt ttttctaaaa gctccggatt gattaacacc agccgttcgt
    20161 cacggttttc cgaaacatca ataacaatga tacgttgatg gatatcaacc tgggttgccg
    20221 ccaggccaat accttcttct gcgtacatcg tctcgaacat atcatcgacg atacgctgaa
    20281 tttctgcatt cacttcttct accggtttag caactttgcg aagccgctcg tccggaatat
    20341 gtaacacttg caaaactgac ataaatctcc agagatgtgt tcaggagtta gaaagattat
    20401 ttcttctatt ctagacaaat ccccctctga ttgacagcat cactgaccaa tcgcaaagat
    20461 tgctaaggct gcttatggca gggagataag gatggtcgat acagatattt ggctgcgttt
    20521 aatgagcatc agcagcttgt acggcgatga tatggtccgt atagctcact ggctggcaaa
    20581 acagtcgcat attgatgcgg ttgtattgca gcaaacaggg cttacattgc ggcaggcaca
    20641 acgctttctt tcatttccgc gaaagagtat cgaaagctca ctttgttggt tggagcaacc
    20701 caaccatcat ttaatccctg cggacagcga attttatcct cctcaacttc tggcgacgac
    20761 agattacccc ggcgcactgt ttgttgaagg agaactgtac gcgctgcatt catttcagct
    20821 tgccgtagtg gggagtcggg cgcattcatg gtatggcgag cgatggggac gattattttg
    20881 cgaaactctg gccacgcgtg gagtgacaat tacgagtgga ctggcgcgtg gaatcgatgg
    20941 tgtggcgcat aaagcggcct tacaggtaaa tggcgtcagc attgctgtat tggggaacgg
    21001 acttaatacc attcatccgc gccgccatgc ccgactggct accagtttgc ttgaacatgg
    21061 tggggcactt gtctcggaat ttcccctcga tgtttcaccc cttgcttaca atttcccacg
    21121 aagaaatcgc attatcagtg gtctaagtaa aggtgtactg gtggtggaag cggctttgcg
    21181 cagtggttcg ttggtgacag cacgttgtgc gcttgagcag gggcgtgaag tttttgcctt
    21241 gccaggacca atagggaatc cgggaagcga agggcctcac tggttaataa aacaaggtgc
    21301 gattcttgtg acggaaccgg aagaaattct ggaaaacttg caatttggat tgcactggtt
    21361 gccagacgcc cctgaaaatt cattttattc accagatcag caagacgtgg cattgccatt
    21421 tcctgagctc ctggctaacg taggagatga ggtaacacct gttgacgtcg tcgctgaacg
    21481 tgccggccaa cctgtgccag aggtagttac tcaactactc gaactggagt tagcaggatg
    21541 gatcgcagct gtacccggcg gctatgtccg attgaggagg gcatgccatg ttcgacgtac
    21601 taatgtattt gtttgaaacc tatattcaca cagaagctga gttgcgtgtg gatcaagaca
    21661 aacttgaaca ggatcttacc gacgcaggat ttgatcgaga agatatctac aatgccctgc
    21721 tttggctgga aaagcttgcg gattatcagg aaggactgac agaaccgatg caactggcct
    21781 ctgatccact ctccatgcgt atttataccc ctgaggagtg tgaaagactg gatgccagct
    21841 gtcgtggttt tctgcttttc cttgagcaaa ttcaggtgct taaccttgaa atccgtgaaa
    21901 tggtgataga gcgagtgctc gcgctggata ccgcagagtt cgacctggaa gacctgaaat
    21961 gggtaatcct gatggtgttg ttcaatattc cgggctgcga aaatgcgtac cagcaaatgg
    22021 aagaattact ctttgaagtg aatgaaggta tgctgcatta atcatttctt taattcagca
    22081 taagttgtta tggcgaaatc agcactgttc acggtgcgta ataatgagtc ctgcccaaag
    22141 tgcggggctg aactggttat tcgatccggg aaacacggtc cgtttcttgg atgctcacag
    22201 tatccggcgt gtgactacgt ccgtcctctg aaatcttcag cggatggaca tattgtcaaa
    22261 gttctggagg ggcaggtttg ccctgcatgt ggcgcaaatc tggtattacg ccagggacgc
    22321 tttggtatgt ttattggttg cagtaactac cctgaatgcg aacataccga acttatcgat
    22381 aaaccggacg aaacagcaat tacatgcccc caatgtcgga cgggccatct ggtccagcgc
    22441 cgctcccgtt atggcaaaac atttcactcc tgtgatcgct acccggagtg tcaatttgcc
    22501 attaacttca aacccatagc tggagaatgc cctgagtgtc attatccact actcatcgaa
    22561 aagaaaaccg cgcagggtgt aaaacacttt tgtgccagta aacaatgtgg aaagccggtt
    22621 tcggcggaat aataacgtga ataataacct gcaaggagac gctatcgcag ctgcgataga
    22681 tgtcctcaat gaagaacgtg tcatcgccta tccaacggaa gccgttttcg gtgttgggtg
    22741 cgatcctgat agcgaaacag cagtgatgag actgttggag ttaaaacagc gtccggttga
    22801 taaggggctg attttaatcg cggcaaatta cgagcagctt aaaccctata ttgatgacac
    22861 catgctgact gatgcgcagc gtgaaaccat tttttcccgc tggccaggtc ctgtcacctt
    22921 tgtctttccc gcgcctgcga caacaccgcg ctggttgacg ggccgctttg attcgcttgc
    22981 tgtacgagtc accgaccatc cgttggtggt tgctttgtgc caggcttatg gtaaaccgct
    23041 ggtttctacc agtgccaact tgagtggatt gccaccttgt cgaacagtag acgaagttcg
    23101 cgcacaattt ggcgcggcgt tcccggttgt gcttggtgaa acgggggggc gtttaaatcc
    23161 ttcagaaatc cgcgatgccc tgacgggtga actgtttcga caggggtaac ataatggaaa
    23221 cctatgctgt ttttggtaat ccgatagccc acagcaaatc gccattcatt catcagcaat
    23281 ttgctcagca actgaatatt gaacatccct atgggcgcgt gttggcaccc atcaatgatt
    23341 tcatcaacac actgaacgct ttctttagtg ctggtggtaa aggtgcgaat gtgacggtac
    23401 cttttaaaga agaggctttt gccagagcgg atgagcttac tgaacgggca gcgttggctg
    23461 gtgctgttaa taccctcaag cggttagaag atggacgctt gcagggtgac aataccgatg
    23521 gtataggttt gttaagcgat ctggaacgtc tgtcttttat ccgtccgggt ttacgtattc
    23581 tgcttatcgg cgctggtgga gcatctcgcg gcgtactact accactcctt tccctggact
    23641 gtgcggtgac aatcactaat cggacggtat cccgtgcgga agagttggct aaattgtttg
    23701 cgcacactgg cagtattcag gcgttgggta tggacgaact ggaaggtcat gagtttgatc
    23761 tcattattaa tgcaacatcc agtggcatca gtggtgatat tccggcgatc ccgtcatcgc
    23821 tcattcatct aggcatttat tgctatgaca tgttctatca gaaaggaaaa actccttttc
    23881 tggcatggtg tgagcagcga ggctcaaagc gtaatgctga tggtttagga atgctggtgg
    23941 cacaggcggc tcatgccttt cttctctggc acggtgttct gcctgacgta gaaccagtta
    24001 taaagcaatt gcaggaggaa ttgtccgcgt gaatcaggcc atccagtttc cggacaggga
    24061 agagtgggac gagaataaaa aatgtgtatg ttttcccgct ctcgtgaatg gtatgcaact
    24121 gacatgcgcg atctctggcg agagtctggc gtatcgcttt actggagata cgccagaaca
    24181 gtggttagcg agttttcgtc agcatcgctg ggacctggaa gaagaagcgg aaaacttaat
    24241 tcaggaacaa agtgaagatg atcaaggctg ggtctggtta ccctgatcca gatattcgtc
    24301 cttccatttc acgtaattat tcgcggaata gcgtaaccca gccttctctt catcacttaa
    24361 cgggcggatc tgtttgacgg ggctaccgag atacagatat ccgctctcca gccgtttatt
    24421 ttgtgggacc agactacccg caccaatcat cacatcatct tctactattg cgccatcaag
    24481 taaaattgag cccatcccaa ccaaaactcg attgccaatg gtgcagccgt ggagcatcac
    24541 cttgtgacca acagtgacat cttcgccaat ggttaatggg ttgccatctg ggttgtacga
    24601 ggatttatga gtgacatgca acatactgcc atcctggata ttggtgcgtg ctccgatctg
    24661 tacataatgt acatctccac gaatcacaac gagcggccag atccccacat catcagccag
    24721 acgaacgcca ccaatcacga cactgctatc gtcgatcatt acgcgctgac cgatttgtgg
    24781 aaaaagatcg cggtatgggc gtaaaacatc agacatactt acctcagcaa taaatgattt
    24841 actaatgact ttgggggcat tattggcctt gtgcaagtct tttagtatgc aaaaaagcac
    24901 cgttttgtgt gcgattgcag caaaaagggt gaaaaaacaa caaacagaaa aaaagatcaa
    24961 aaaaatactt gtgcaaaata ttgggatccc tataatgcgc ctccgttaag acgacaacgt
    25021 gaaacacttc acaggatggt cggaacaacg aagagaaaaa atcctgaaat tcagggttga
    25081 ctctgaaaga ggaaagcgta atatacgcca cctcgcgaca gtgagctgaa agccgcgtcg
    25141 caactgctct ttaacaattt atcagacaat ctgtgtgggc actcgaagat acggattctt
    25201 aacgtcgcaa gacgaaaaat gaataccaag tctcaagagt ga
//
LOCUS       DAJTMC010000044        25009 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.044, whole genome shotgun sequence.
ACCESSION   DAJTMC010000044 DAJTMC010000000
VERSION     DAJTMC010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25009
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.044"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            <1..808
                     /locus_tag="OO130_001627"
     CDS             <1..808
                     /locus_tag="OO130_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HCR5365085.1"
                     /translation="PRWLMSRGKQEQAESILRKIMGNTLATQAVQEIKHSLDHGRKTG
                     GRLLMFGVGVIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVIN
                     LTFTVLAIMTVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQAPGIVALLSMLFYVAAF
                     AMSWGPVCWVLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHN
                     GFSYWIYGCMGVLAALFMWKFAPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(852..1250)
                     /gene="psiE"
                     /locus_tag="OO130_001628"
     CDS             complement(852..1250)
                     /gene="psiE"
                     /locus_tag="OO130_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HCR5365086.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLIIVDHK
                     SPLDVLIYSVAILLLVITLWLCNSKRLKRE"
     gene            1520..1798
                     /locus_tag="OO130_001629"
     CDS             1520..1798
                     /locus_tag="OO130_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5365087.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            complement(1845..3941)
                     /locus_tag="OO130_001630"
     CDS             complement(1845..3941)
                     /locus_tag="OO130_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HCR5365088.1"
                     /translation="MKKRHLLSLLALGISMACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIGGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYNDNARPQYQPQPQDAILQHSVVANQLTLLKYNVGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETKLAQKRVEPVVPQSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     MADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVLKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(3941..4678)
                     /locus_tag="OO130_001631"
                     /pseudo
     CDS             complement(3941..4678)
                     /locus_tag="OO130_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418452.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
     gene            complement(4675..5313)
                     /locus_tag="OO130_001632"
     CDS             complement(4675..5313)
                     /locus_tag="OO130_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HCR5365089.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            complement(5427..5669)
                     /locus_tag="OO130_001633"
     CDS             complement(5427..5669)
                     /locus_tag="OO130_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5365090.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAGSAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            complement(6131..7780)
                     /gene="pgi"
                     /locus_tag="OO130_001634"
     CDS             complement(6131..7780)
                     /gene="pgi"
                     /locus_tag="OO130_001634"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HCR5365091.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     gene            8305..9654
                     /gene="lysC"
                     /locus_tag="OO130_001635"
     CDS             8305..9654
                     /gene="lysC"
                     /locus_tag="OO130_001635"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HCR5365092.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNDGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
     gene            9906..10178
                     /locus_tag="OO130_001636"
     CDS             9906..10178
                     /locus_tag="OO130_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HCR5365093.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(10311..11183)
                     /gene="rluF"
                     /locus_tag="OO130_001637"
     CDS             complement(10311..11183)
                     /gene="rluF"
                     /locus_tag="OO130_001637"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HCR5365094.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            11531..12478
                     /locus_tag="OO130_001638"
                     /pseudo
     CDS             11531..12478
                     /locus_tag="OO130_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
     gene            12548..13354
                     /locus_tag="OO130_001639"
     CDS             12548..13354
                     /locus_tag="OO130_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR5365095.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSAKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALVWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTHG"
     gene            13364..13771
                     /locus_tag="OO130_001640"
     CDS             13364..13771
                     /locus_tag="OO130_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIA"
                     /protein_id="HCR5365096.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGDARVEAVEFVPGENAGDIV
                     AKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATAEEDF"
     gene            13771..14265
                     /locus_tag="OO130_001641"
     CDS             13771..14265
                     /locus_tag="OO130_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HCR5365097.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIDTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            14331..15128
                     /locus_tag="OO130_001642"
     CDS             14331..15128
                     /locus_tag="OO130_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HCR5365098.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSAFGVQALRVAIPALIVSL
                     FVSADMVSNMLSAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWRKAEPQLQATASTALDQLDD"
     gene            15139..15981
                     /locus_tag="OO130_001643"
     CDS             15139..15981
                     /locus_tag="OO130_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000437910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCR5365099.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRRHLVFFNTTPAVCGPVIGVTAAMEEARANGAEIDDGTINGIKVG
                     LMGPLAGVGDPLVWGTLRPITTALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQL
                     GFRKGVNIVSDMGGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGST
                     VTMTVQNILDQLCPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLSRF
                     FAPA"
     gene            16015..17244
                     /gene="sorE"
                     /locus_tag="OO130_001644"
     CDS             16015..17244
                     /gene="sorE"
                     /locus_tag="OO130_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HCR5365100.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVATNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVGGEATHVVIPNEVMEQDCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGRMLILGGTGPMGLLAIDYALHGPVNPSQLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNAADAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEAEQYLLTHAEAISHD"
     gene            17627..18316
                     /gene="pepE"
                     /locus_tag="OO130_001645"
     CDS             17627..18316
                     /gene="pepE"
                     /locus_tag="OO130_001645"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HCR5365101.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEDNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(18407..20038)
                     /locus_tag="OO130_001646"
     CDS             complement(18407..20038)
                     /locus_tag="OO130_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HCR5365102.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTAALTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQSDEDEGRDEY"
     gene            complement(20258..23946)
                     /gene="metH"
                     /locus_tag="OO130_001647"
                     /pseudo
     CDS             complement(20258..23946)
                     /gene="metH"
                     /locus_tag="OO130_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312964.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
     gene            24146..24970
                     /gene="iclR"
                     /locus_tag="OO130_001648"
     CDS             24146..24970
                     /gene="iclR"
                     /locus_tag="OO130_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HCR5365103.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
BASE COUNT         5883 a         6615 c         6532 g         5979 t
ORIGIN      
        1 tcctcgctgg ctgatgtcac gcggcaagca agaacaggcg gaaagtatcc tgcgcaaaat
       61 tatgggcaac acgcttgcaa ctcaggcagt acaggaaatt aaacactccc tggatcatgg
      121 ccgcaaaacc ggtggtcgtc tgctgatgtt tggcgtgggc gtgattgtca tcggcgttat
      181 gctctccatc ttccagcaat ttgtcggcat caatgtggtg ctgtactacg cgccggaagt
      241 gttcaaaacg ctgggggcca gcacggatat cgcgctgttg cagaccatta ttgtcggagt
      301 tatcaacctc accttcaccg ttctggcaat tatgacggtg gataaatttg gtcgtaagcc
      361 actgcaaatt atcggcgcac tcggaatggc aatcggtatg tttagcctcg gtaccgcgtt
      421 ttacactcag gcaccgggta ttgtggcgct actgtcgatg ctgttctatg ttgctgcctt
      481 tgccatgtcc tggggtccgg tatgctgggt actgctgtcg gaaatcttcc cgaatgctat
      541 tcgtggtaag gcgctggcaa tcgcggtggc ggcccagtgg ctggcgaact acttcgtctc
      601 ctggaccttc ccgatgatgg acaaaaactc ctggctggtg gcccatttcc acaacggttt
      661 ctcctactgg atttacgggt gtatgggcgt tctggcagca ctatttatgt ggaaatttgc
      721 cccggaaacc aaaggtaaaa cccttgagga gctggaagcc ctctgggaac cggaaacgaa
      781 gaaaacacaa caaactgcta cgctgtaatc ttcctgtcca gcacgccgcg ccagttcggc
      841 gtgctgtctt tttactcccg cttcagccgt ttcgaattac acagccacag agtgatcacc
      901 agtaacagga tcgcaaccga gtagatcaac acatccagtg gcgatttatg atcgacgatg
      961 atcaagcgca caatggcggt gatcccaata tagacaaagt aacgtagcgg gaagtgaaaa
     1021 ccggactgaa agtacttcac aatcagcgcg ataaattcga aatagagaaa gtaaaccacc
     1081 agctcatatt tgctggtttg ttctggcgcg aacagcacat ctgccagatg caccgtttct
     1141 ttgccgagga agacaaccaa aatcaggccg aggcacagca ggccaagatt gagtacggtc
     1201 tgcaaaatgg tggagataaa ctccacgcgc ggacgagaga gtgacgtcat agcagtacct
     1261 ccttcacagt ggcgagcttc ctgtataacg caaaaatgtg acggagatct atattttttg
     1321 tgtatgtttt gttcaacctg gatttggagc gcgcctccac caaagccgga tatacctacc
     1381 cgtaggccgg ataagccgcg cctggcggcg catccggcaa gacaaaccgc acccaagccg
     1441 gtaattcaaa aatcacccat tcaggtgatt cccagccacc agcaatgcgc gaaaaatcga
     1501 caatgttcct ctggaacaaa tgaaacgtaa cctgataaag gtggtaaaaa tgaagcctta
     1561 ttttgctgct ttgatgttat cagtctctgt tcttcctgct tatgctggtc cgctcggtac
     1621 tgctgataaa gccgacctac cgcaaagcaa tgtctccagc ccaatgatgg cccagtcgct
     1681 caggcaacca gacctgcaac cgatatcgac ggacaggaag acagaatgtt ttcgccttta
     1741 cacaccagat cgcaaaccgg gggtgaactg cgttcctgat ggctcgacag gccattaaga
     1801 atgaaggtgc cagtgagtgc gaaaactggc acctttccca tgacttagcg gaaattgacg
     1861 ctacggtcgc tggtcatgtc atacaactgg aacttacgcc caagttgctg accaccgtca
     1921 cgcgtcagcg gcgtccagcc aatcgccgca cggctgcgtg tcgggccaga cgagaagaga
     1981 tccaacggta ccgagacata cacgcctttg gtgaagtccc cttcgccgta ctcctctttc
     2041 aaaacgttgg tgatcgtggc atagccaccc accaccacgc cgctgtcaaa gcgtttggcg
     2101 atctccagcg tgccgccttt atcccctgcc agatactgcc cgacgctggc tttcaccaac
     2161 acgtcctggg cgaaagatgg cgtccagtag gcggtcagat gtccggtttt cacgctgtag
     2221 tcggtgaatt tcatcatatc ttttgcacta cgccagtcgc gctgtttaac gtagttggca
     2281 tccagaccaa acgcccagtt actgtccagc ggacgataca acacttcggc ccccgcaccg
     2341 ccaaacatgg tttcgagata acctccatag acctgaccgt agaagccgtt gcccagatgc
     2401 tggaagtagt tggcttgcag gttattcaca tagacatcgt tctgcacata ctcgcgcaca
     2461 tgggtacgaa cgcgcggtaa gtgcgagtcc tgcggcggat tggtgtagtt aaacttgtcg
     2521 tagttgttgg caagatttgc aaacaggctg ccggtggtca gcagatggtc cgtcagccac
     2581 aaatctgcca ttcccatcac gcccagctga tacatgtaaa agttttccgg gccaccgacc
     2641 gactggttca gcaccggatc gatatggaaa tcaaagcgtg atttgtcgat ataccagccc
     2701 tgctcggtgg actgcggaac cactggctcg acgcgttttt gcgccagctt cgtttcgtgg
     2761 cccaacggct ctccggcgag atgatttttc aggctggcga catcggtttc cgtcgtcacc
     2821 tgcggcatgt taagacgatt ttccgtaatg cggatcgttt tgatcccatc cggcagatcg
     2881 ttcatcacga tccgattagc acggatgatc ccttcgcgcg aatcacgata tttcacctgc
     2941 tcgccggtga catacagcgt atcgcctttc gcctggatct gcggatcggc aagtccgaca
     3001 ttgtatttca acagcgttaa ctgattcgcc accaccgaat gctgcaaaat ggcatcctgc
     3061 ggctgcggtt gatattgcgg gcgggcgtta tcgttgtaag acgggcgcag atcgttaaag
     3121 ttggtgcgca gcgtgacgcc aaacataaag gtgttgccac gttcatagct aaggttaacg
     3181 tcggcccaat cggtaacgcg ataaatcgca ccgacgttaa acttactttt ttgctccagc
     3241 ttcccggcaa aatcctgctg ataattattg ccttcatact ccagtttcag gcgcagcggt
     3301 tgccagggcg tctggtattc cacgccgcca aacagtgagg caggaccgtg gaacatctgg
     3361 ctaccgccga tagatcccgc ctgtttgtag ctgttatcgc gatagcaata tttatcactg
     3421 gctgaacaga gcggattttt cacattaccg ctggtgccca gataacccca gcccaggccg
     3481 agcgtaaaat cgaacggccc ccaggctttg ctggcaacaa gatattccgc atcaaacagc
     3541 cccgtaccgc cgatatccct cgcaccaacc gccacttgcg gcagccagta actctcttcc
     3601 cacaaacgca gtttgagatc gaaggcttta tctttatacg tttgatcgcc agagaacgct
     3661 tcgacgctgc tgtactgccg ggtgcgcacg tcggtgtagc gcagcgttgt ttccagccac
     3721 gggaagagtt gcactgaagc tgagtaataa cggtactgat cgttatcgcg atagttcagg
     3781 ctcaactccc cttcccgcgc catgcgtgcg gtgggcgttt gtaataatcc tacgccaccg
     3841 aaatccgact gcgacgggcc aatgggcgca ggatatgttt cgccgtagca agccatgcta
     3901 atgcccagcg ccagtaagct aagcagatgt ctttttttca ttattggggt atccgctgcg
     3961 tcagagtctg aagaatgtcg gcgttaaggg catcaggcgt ctcactccag acggagtccg
     4021 cgaggccaac ataaataatg ctgccgggca tcggctctac gtgacgcttg ttccagtaag
     4081 ccaccggcgc tttttgcgtg cgtccgtccg ggtaaaccac ccacgcgtag ctgcgatccg
     4141 caccgctaag caggttctgc ccggagagat agctcgccac gtcgcgacct ggagtgaatg
     4201 gctgcttgcc aggacggctg ataagcccga acaacgtgac cgtggacggc ggtggtccga
     4261 cccacagcgt gtagttgccc tgcaacggtg ggttaccgcg ttcggcaacg cgcacgatat
     4321 cgggatcaag attgattttt tgcctgcccg tcactttcaa cgcctgaatt tgctggcgta
     4381 aggcgttaat ggcggcggca tcgtcggcgc tggagtctgc ggcctgttca gccagccgtg
     4441 tcagcaacgc ctgctgctga cgcaatgctg ccgccgttgc cagctcttcg ctaatcaccg
     4501 caccgggcca ccagttgttt gccagccgcg gttagcctac cagatcgatt aaatgctctg
     4561 cgcctgttag tgttttggcc tcactgctgc cattgctgaa caccttgacg gttcctgccg
     4621 cgaagaccga tgacgctccc acactcaaaa gcaacgcgac aaaagtttgt ttaatcattg
     4681 ttttgccgcc ttgatgagag tggttttcac cgggaaataa tccgcgccga gatactgttc
     4741 cgactggcga atttgccctt cgctgtcgat ccagtagcgg ttatgccagc gcgcctggtc
     4801 ggtggagact tcttcgtcca gcacgcgaac caaggtttca tcgctgccga ctttgacggt
     4861 atcggtgcca tcccatttga agactgagcg tgcggtggcg tagcgtacct gctggtactc
     4921 ggtccagccc atcgtgcgcg tccatgttgc accatcaaca atttgtgcgg gtttaatcag
     4981 cgggtcggcg gcaaggttat tcacttcaat caggttgtcg ccgccaagca aagtcttcac
     5041 cagacgacca tgttgtgtga caagagtggc ttgatcctga gtgacccatt tttgttgtcc
     5101 gtcttcagcg aaggccagca ccacaaacaa ttgcggcccg ccattaagct gcatgtactg
     5161 gctggcgtag ggcatatttt gaatatcatc gtccgtcagc tgtacgcctg gcgtgccgaa
     5221 cagactgtcc cacagtgaat tgcccagctc tttagtcgtg gctgaacagg cctgtaatag
     5281 caggcagata agaatgagtg caggtcgctt cacgactctt ctccagaggg gcagaataac
     5341 cacaccggag tgtggttatg gtaaatccct tcatacttca agccacagat gcaatgactc
     5401 aaattatttt ggggatacat actttattac tgggtactgg tggtcgtggt tgtggtggtc
     5461 ccggtattag aaccatcacc gccaccggtt gccgcgagcg cgacacccac cgccgagctt
     5521 acggtgctga cgctggtcgc ggaggaacta cccgccgaaa ccgacgttgc tgccgaccct
     5581 gccgcttcgc ccacctgtac aggtgcagcc cacgcagaag tcgccgcaag cgcagatatg
     5641 gcaaaaatgc catacagaac ttttttcata acaatttccc ttcattgaat gaatggagat
     5701 ttacccaaaa acatttcggg cgggatcgag tatacacaac taaagcatgc gaattacggc
     5761 gcggggaatt agcgatggcg ttttaggata attggaatga atatcaaata taagcttcaa
     5821 cttatattta aataacaata aaaatcataa acttaaaata aaaacaaaag taattaatct
     5881 gcgtattctc cgaaataaac aacgattaaa aatctatttt aggattaacc tcacggtatg
     5941 atttccgtta aatcacagac aaggggattt atctgataaa acaatgccga cttattgtcg
     6001 gcattgtttt taaggttatt taactcctca tactatgcat taatgtcgat attggcatca
     6061 ggctccggtt gccggatgcg gcgtaaacgc cttatccggc ctacatatcg gcatcaggca
     6121 cgcatgatga ttaaccgcgc cacgctttat agcggttaat cagaccattg gtcgagctat
     6181 cgtggctgct gatttctttg tcatctttca gctctggcag aatacggttc gccagttgtt
     6241 tacccaactc cacgccccac tggtcgaagg taaagatgtt caggatcacg ccctgagtaa
     6301 agattttgtg ctcatacagc gcaatcaacg cccccaggct gaacggagtg atttcgcgca
     6361 gcaggatgga gttggtcggg cggttacctt cgaacacttt gaacggcacc acgtagtcaa
     6421 gcgttgccgg atctttaccc tgatcgcgat attcctgctc aaccacttcg cgggatttac
     6481 caaacgccag cgcttcggtc tgggcgaaga agttagacag cagtttctgg tggtgatcag
     6541 agagcgggtt atgggtgata gccggggcga tgaaatcgca cggtaccatt ttggttccct
     6601 ggtggatcag ctggtagaac gcgtgctgac cgttagtgcc tggttcaccc cagataatcg
     6661 ggccagtctg gtaatccaca acgttaccgt tacggtcaac atacttaccg ttggactcca
     6721 tattgccctg ctggaagtac gccgcgaaac ggtgcatata ctggtcatac ggcagaatcg
     6781 cttcagtttc cgcaccaaag aaattgttgt accagatgcc aatcagcgcc agcaggacag
     6841 gcaggttttt ctcggcaggc gtggtggaga aatgcttgtc catcgcgtgt gcgccagaaa
     6901 gcagttcaac gaagttatca aagccgatgg agagaacaat cgacaggcca atcgctgacc
     6961 acaaagagta acggccgccg acccagtccc agaactcgaa catgttggca gtatcaatac
     7021 caaactcgcc aacggctttg gcattggtgg aaagcgccgc aaagtgtttt gcaacgtgtt
     7081 tttcatcacc tgccgctttc aggaaccagt cacgcgcgct atgggcgttg gtcatagttt
     7141 cctgagtggt gaaggtttta gatgctacca agaacagcgt ggtttccggg tttacttttt
     7201 tcagcacttc agcgatgtga gtcccatcga cgttagaaac aaagtgcatg ttcaggtgat
     7261 ttttgtacgg acgcagtgct tcggtcacca tgtacgggcc gaggtcagaa ccaccgatcc
     7321 cgatgttcac tacgtcagtg attgctttgc cggtataacc cttccactca ccggagataa
     7381 tcgcttccga gaaggttttc atcttctcca gcaccgcgtt gacttccggc attacgtctt
     7441 tgccatcaac cagaatcggg gtattgctac ggttacgcag cgctacgtgc agcacggcgc
     7501 ggttttcagt gcggttgatc ttctcgccag agaacatcga cttaatcgcg cccgccagat
     7561 cgcactcttt cgccagatcc tgtaatttcg ccagcgtctc ttcagtgatg cggtttttgg
     7621 agtaatccac cagcatctga tcgtcgaatg ttgcggagaa cttagaaaaa cgatcgccgt
     7681 ctttagcaaa aagatcggcg atcgtaacgt ctttcatttc atcgaagtgt ttctgtagtg
     7741 cctgccaggc agcggtctgc gttggattga tgtttttcat tagcaatact cttctgattt
     7801 tgagaattgt gactttggaa gattgtagcg ccagtcacag aaaaatgtga tggttttagt
     7861 gccgttagcg taatgttgag tgtaaaccct tagcgcagtg aagcatttat tagctgaact
     7921 actgaccgcc aggagtggat gaaaaatccg catgaccccg tcgttgacaa ccgccccgct
     7981 caccctttat ttataaatgt actacctgcg ctagcgcagg ccagaagagg cgcgttgccc
     8041 aagtaacggt gttggaggag ccagtcctgt gataacacct gagggggtgc atcgccgagg
     8101 tgattgaacg gctggccacg ttcatcatcg gctacagggg ctgaatcccc tgggttgtca
     8161 ccagaagcgt tcgcagtcgg gcgtttcgca agtggtggag cacttctggg tgaaaatagt
     8221 agcgaagtat cgctctgcgc ccacccgtct tccgctcttc ccttgtgcca aggctgaaaa
     8281 tggatcccct gacacgaggt agttatgtct gaaattgttg tctccaaatt tggcggtacc
     8341 agcgtagctg attttgacgc catgaaccgc agcgctgata ttgtgctttc tgatgccaac
     8401 gtgcgtttag tagtcctctc ggcttctgct ggtatcacta atctgctggt cgctttagcg
     8461 gaagggctgg aacctggcga gcgattcgaa aaactcgacg ctattcgcaa catccagttt
     8521 gccattctgg aacgtctgcg ttacccgaac gttatccgtg aagagattga acgtctgctg
     8581 gagaacatta ctgttctggc agaagcggcg gcgctggcaa cgtctccggc gctgacagat
     8641 gagctggtca gccacggcga gctgatgtcg accctgctgt ttgtcgaaat cctgcgcgaa
     8701 cgcgatgttc aggcacagtg gtttgatgta cgtaaagtga tgcgtaccaa cgaccgattt
     8761 ggtcgtgcag agccagatgt agccgcgctg gcggaactgg ccgcgctgca gctgctccca
     8821 cgcctcaatg acggcttagt gatcacccag ggatttatcg gtagcgaaaa taaaggtcgt
     8881 acaacgacgc ttggccgtgg aggcagcgat tatacggcag ccttgctggc ggaggcttta
     8941 cacgcatctc gtgttgatat ctggaccgac gtcccgggca tctacaccac cgatccacgc
     9001 gtagtttccg cagcaaaacg cattgatgaa atcgcgtttg ccgaagcggc agagatggca
     9061 acttttggtg caaaagtact gcatccggca acgttgctac ccgcagtacg cagcgatatc
     9121 ccggtctttg tcggctccag caaagaccca cgcgcaggtg gtacgctggt gtgcaataaa
     9181 actgaaaatc ctccgctgtt ccgcgcgctg gcgcttcgtc gcaatcagac tctgctcact
     9241 ttgcacagcc tgaatatgct gcattctcgc ggcttcctcg cggaagtttt cggcatcctc
     9301 gcgcggcata atatttcggt agacttaatc accacgtcag aagtgagcgt ggcattaacc
     9361 cttgatacca ccggttcaac ctccactggc gatacgttgc tgacgcaatc tttgctgatg
     9421 gagctttccg cactgtgccg ggtggaagta gaagaaggtc tggcgctggt cgcgttgatt
     9481 ggcaatgacc tgtcaaaagc ctgcggcgtt ggcaaagagg tattcggcgt actggaaccg
     9541 ttcaacattc gcatgatttg ttacggcgca tccagccata acctgtgctt cctggtgccc
     9601 ggcgaagatg ccgagcaggt ggtgcaaaaa ctgcatagta atttgtttga gtaaatactg
     9661 tatggcctgg aagctatatt tcgggccgta ttgattttct tgtcactatg cttatcaata
     9721 aacgagcctg tactctgtta accaacgtct ttatcgagaa taattgcctt taattttttt
     9781 atctgcatct ctaattaatt atcgaaagag ataaatagtt aagagaaggc aaaatgaata
     9841 ttatcagttc tgctcgcaaa cgaattcccg tacactgaac tctctggctc actgagggca
     9901 ccatcatggc actcccccgt attacccaaa aagagatgac tgaacgcgag cagcgtgaac
     9961 ttaaaacact gctggatcgg gcgcggattg cgcatggtcg cgtactgacc aactccgaaa
    10021 ccaacagcat taagaaagag tacatcgata aattaatggt cgagcgtgag gctgaagcga
    10081 aaaaagcccg ccaattgaag aaaaagcagg cttataaacc ggatcctgaa gcgtcattta
    10141 gctggtcagc aaatacgtcg acgcgcggca ggcgctaatg aacaatcaac gcctgagatt
    10201 ttcactcgtc tgtgtggaca caatcttccc ctcaccctaa ccctctcccc gaaggggcga
    10261 ggggactgtc cgagctcttt ttggacgttt tcatcagtct gaggcgttaa tcagcgtccc
    10321 ttctttttac gccccggcga ggtaaaacgc ttaccgttgg acgccgggcg accgcctttt
    10381 tccgccgttt tttccatctt aacgactgga cgtttgatgc ccgctgtttt cggtttggcc
    10441 ttcgctttag gttttgcctc ggaagaggaa ttttcgatga gcttaaagag gtcgattaac
    10501 tcatcgtcgg ttaaatcgcg ccactccccc agcggaatgc cgcttaagct gacgttcata
    10561 atgcgcgtac gttccagctt tttcacttca tagccgaaat gctcgcacat gcgccggatc
    10621 tgacggttca gcccctgcac cagggtaatg cggaagacaa acggcgcttc ttttttgact
    10681 ttgcactttt tcgtcaccgt cccgaggatc ggcacccccg cccccatacc acgaataaat
    10741 tcatcggtaa tcggtttatc gaccgtcacc agatactctt tctcatgatc gttacccgca
    10801 cggaggatct tattcaccag atcgccgtga ttggtgagga aaatcagccc ctgggagtct
    10861 ttatccaggc ggccaatcgg gaacacgcgt ttgctgtggt taacgaaatc gacaatgtta
    10921 tcgcgctcgc catcttcggt ggtgctgaca ataccgacgg gcttgttcag ggcgataagt
    10981 accaaatctt cggcttcccg aggctcaatc aactgaccgt ttactttcac aatgtcgccg
    11041 ggtttcacct gatcgccaat ggtggctcgc ttgccattaa ggaacacatt gccttgctcg
    11101 atatagcgat ccgcttcgcg gcgtgagcaa attccgcttt cgctgatgta tttatttaaa
    11161 cggactgatg agtcgggcag cataaattct cctgtaaaac gcggaatata ccgtacctct
    11221 tagcggggaa aaacagggtt atgcggcaac gtcgaaatta aatgtgatct gccacgcctt
    11281 ttatcacaaa agtgtctccg gaagtacgat gcaaatttgc ctggcaaaat aaggataatc
    11341 aacatattac gaatcgctgc acacctgtgc tgatgactac tacgcagaga aacaaatgtg
    11401 caagagaaag cgctatatct ccccatgcaa agagtgaaaa atcgctttga aaggccctca
    11461 tgatggggtt atatcagatc aagcggtgag aaagcatatt tgcacaaatg tgcagaacca
    11521 ggaggcggca atggaaaaca gtgacgatat ccgtttgatt gtgaagattg cccaactcta
    11581 ttacgaacag gatatgacgc aggcgcaaat cgcgcgcgaa ctgggtattt accgcaccac
    11641 catcagccgc ttgcttaaac gaggccgcga tcagggaatt gtcaccatcg ccatcaacta
    11701 tgactacaac gaaaatctct ggctggagca gcaactgaag caaaagtttg gcctgaaaga
    11761 cgttgtggtg gtgtcgggaa atgatgagga tgaagagact caactggcga tgatggggtt
    11821 acacggcgcg caactgctgg atcgcttgct ggagcctggc gatattgtcg gtttttcctg
    11881 gggtcgcgcg gtgagcgcac tggttgaaaa cttgccgcag gcggggcaat cgcggcagtt
    11941 aatctgcgta ccaattattg gcggaccgtc cggtaaactc gaaagccgct atcatgtaaa
    12001 cacattaacc tacagcgcgg cagcgaagct gaaaggggaa tcgcatctcg cggattttcc
    12061 ggctctgctg gataacccat taattcgtaa tgggatcatg cagtctcagc actttaaaac
    12121 catctctgcc tactgagata atctggatgt cgccctggtg ggaattggct caccggccat
    12181 tcgcgacggc gctaactggc atgcgtttta tggtggtgaa gagagtgacg acctgaatgc
    12241 ccgccaggtt gctgacgata tttgctcgcg cttttttgat attcacggcg caatggttga
    12301 aacgaatatg agcgaaaaaa cactctctat cgaaatgaat aaattaaagc aggcacgata
    12361 ttccattggc attgccatga gtgaagaaaa atacagcgga attgttggtg cactgcgtgg
    12421 aaaatatatt aattgtctga taacgaatag cagcacagct gaactattac tgaaataaaa
    12481 atcttcccga tgtttagcgt aataaaaatt acggcggggc cctattatct aaaatcagga
    12541 gtctgttatg caaacgtggt taaatttgca ggataaaatc attattgtca ccggcggcgc
    12601 atccggtatt ggtctggcga ttgtagagga attattagca caaggcgcaa acgtacagat
    12661 ggtagatatt cacggtggcg atggtcaata tgaaggtcat aaaggctatc agttttggcc
    12721 gaccgatatt tccagcgcca aagaggtaaa tcatacggta gcggaaatta tccagcgttt
    12781 tggtcgcatc gacggtctgg tcaataacgc cggggtcaat ttcccgcgtc tgctggtcga
    12841 tgagaaagcg cctgccgggc agtatgaact caacgaagct gcattcgaaa aaatggtcaa
    12901 tatcaaccag aaaggcgttt ttctgatgtc gcaggcggtg gcgcgacaga tggtcaaaca
    12961 acatgatggc gtgattgtga atgtttcctc agaaagtgga ctggaaggct cagaaggcca
    13021 aagctgttac gccgctacca aagccgcgct taatagcttc acgcgatcct ggtcgaaaga
    13081 attgggtaaa cacggtatcc gtgtggtcgg tatcgcgccg gggattctgg aaaaaacagg
    13141 actgcgcacg ccggaatatg aagaagcgct ggtgtggacg cgcaatatca ccgtcgagca
    13201 gctgcgtgaa ggctatacca aaaacgccat tcctattggg cgcgccggaa gattagcaga
    13261 agtcgctgat tttgtttgtt atctgctgtc tgaacgcgcc agctatatca ccggagtaac
    13321 cactaacatt gcgggcggca aaacgcacgg ctaaggaggc tttatggtca atgccatttt
    13381 ttgcgcccac ggcaaattag cttgcgccat gctggaatcg gttcagatgg tgtacggcga
    13441 cgccagggtc gaggcagttg aatttgtgcc cggcgagaac gccggagaca tcgtagcaaa
    13501 gctggaaaag ttagtaagca ttcacaatca agatgagtgg ctgattgccg tggatttaca
    13561 gtgcggcagc ccgtggaatg cggcagcgat gcttgcgatg cgcaatcctc gtctgcgggt
    13621 aatcagcggc ctttctctgc cgctggcgct cgaactggtt gataaccagg acagcatgaa
    13681 tgttgatgaa ctgtgtgaac acctgacgca aatcgcaaag caaacctgcg ttgtctggaa
    13741 acaactggca acggcagagg aggatttctg atgaacatta cgctcgcccg cattgatgac
    13801 cgcctgatcc acggtcaggt cactaccgtc tggtcgaaag tggctaacgc ccagcgcatt
    13861 attatctgca atgacgaagt ttataacgat gaagttcgcc ggacattatt gcgtcaggct
    13921 gcgccaccgg gaatgaaggt taacgtggtc aatattgaaa aagccgtcgc tgtttatcac
    13981 aacccgcaat atcaggacga aaccgttttt tatcttttca ctcgcccaca ggatgcctta
    14041 gcgatggttc gccaaggggt aaaaatagac accctaaata ttggcggcat ggcctggcgg
    14101 cccggtaaaa aacagttaac caaagcggtt tcattagatg atgatgatat taacgcattt
    14161 catgagttaa ataatcttgg cgtcatactg gatttacgcg tcgtagcgtc agatccctca
    14221 atcaatatta tcgacaaaat aaacgaacag ttaatcgcaa attaaataat agcgcttgtg
    14281 tacatatgct ttaacaggca gggatgacga cacttaaaag gtgaaaaatc atggaaataa
    14341 gtaccctaca aatcattgct atatttcttt tttcctgtat tgccggaatg ggcagcgtgc
    14401 tggatgaatt tcagactcat cgcccgttaa ttgcctgtac ggtgattggt ttaattctcg
    14461 gtgatttaaa aaccggaata atactcggtg gtacgctgga attgatagct ctcggctgga
    14521 tgaacgtcgg cgcggcgcaa tctccggatt ctgcactcgc cagcataatc tccgccattc
    14581 tggttatcgt tggtcagcaa agcatcgcca ccggaatcgc catcgcgttg cctgtggctg
    14641 cggcaggcca ggtgctgact gtgtttgccc gtaccatcac cgtagtgttc cagcacgcgg
    14701 cggataaagc agcggaagaa gcacggtttc gcactctcga tattctgcat gtctccgcgt
    14761 ttggcgtgca ggcgctgcgc gttgctattc cggcactgat tgtctcactg ttcgtcagcg
    14821 ccgatatggt gagcaatatg ctgagcgcga taccggaatt cgtgacgcgc ggtctgcaaa
    14881 ttgctggcgg ttttatcgtg gtggtcggtt acgccatggt gctacgtatg atgggcgtga
    14941 aatatttgat gcctttcttt ttcctcggtt tcctcgcagg tggctacctc gatctcagtc
    15001 tgctggcgtt cggtggcgtc ggcgtgatca tggccctggt ctacatccag ctaaatccac
    15061 agtggcgtaa agctgaacct caactccagg ccaccgcctc caccgccctt gaccaacttg
    15121 acgattaacg gagccatcat ggaacagaga aaaattacac gcagcgatct ggtgagcatg
    15181 tttctgcgct ccaacctgca acaggcgtcc tttaactttg aacgtattca cgggctgggt
    15241 ttttgctacg acatgatccc cgccatcaaa cgactttacc cattaaaaga ggatcaggtt
    15301 gcggcgctca ggcgacacct ggtgttcttc aataccacgc cagccgtatg tggcccggtc
    15361 atcggcgtca ctgccgccat ggaagaggcg cgggccaacg gcgcggaaat tgatgacggt
    15421 accattaacg gcatcaaagt cggtctgatg ggaccattgg caggagttgg cgatccactg
    15481 gtctggggaa cgctgcgccc gattaccacc gcgctcggcg catctctggc actttcgggc
    15541 aacattctcg gcccgctgct gttcttcttt attttcaatg cggtgcgtct ggcgatgaag
    15601 tggtatggcc tacagctcgg ctttcgcaaa ggggtgaata tcgtcagcga tatgggcggg
    15661 aatgtgctgc aaaaactcac cgaaggcgcg tcgattctcg gactgtttgt gatgggcgtg
    15721 ctggtcacca aatggacgtc aatcaacgta ccgttggtgg tttcacaaac gcatgccgcc
    15781 gatggctcca ccgtcaccat gaccgtgcag aacattctcg accaactttg ccctggtttg
    15841 ctggcgctcg gtctgacgct gcttatggtt cgtctgctca acaaaaaaat taacccggta
    15901 tggctgattt tcgccctgtt tggcttaggg attatcggta atgcgctggg cttcctgtcc
    15961 agattcttcg ccccggcatg actgccgggg ccatcgctca acatgaggtg gtttatgaaa
    16021 acaacagctc tgcgtcttta tggtaaacgt gatttacgcc tggaaacctt tgaccttcct
    16081 gaaatgcagg aggatgaaat cctcgcgacg gtggtcactg acagcctgtg cctctcttcc
    16141 tggaaagagg ccaatctggg tgaaaaccat aaaaaagtac ccgacgatgt ggcgaccaac
    16201 cccatcatca tcggccacga gttttgcggc gatattctgg ccgtgggtaa aaagtggcag
    16261 cacaaattcc agccgggtca gcgttatgtg attcaggcca acctgcaact ccccgaccgc
    16321 ccggactgcc ccggctactc cttcccgtgg gtgggcggcg aagccacgca tgtggttatt
    16381 cccaacgagg tcatggaaca agattgcctg ctggcatacg acggcgaaac ctattttgaa
    16441 ggctcactgg ttgaaccgct ttcctgcgtg attggcgcgt tcaacgccaa ctatcatctt
    16501 caggaaggta gttataacca cacgatgggg attcgcccgc aagggcgcat gctgatcctc
    16561 ggcggcaccg gaccaatggg actgttggcg attgattatg cgctacatgg acccgttaac
    16621 ccgtcgcagc tcgtcattac cgataccgac aacgataaat tgagttatgc gcgcaagcac
    16681 tatccatcag aaccgcaaac actgattcat tatctcaatg ccgccgatgc agcatttgat
    16741 acgctaatgg cgctgagtgg cggtcacggc ttcgatgata ttttcgtctt tgtgcctaat
    16801 gaaggactgg tgactctcgc ctcttccttg ctggcgacag atggttgcct gaatttcttc
    16861 gccggaccgc aggataaaca tttcagcgcg ccaattaatt tctacgatgt gcattatgca
    16921 tttacccact acgtgggcac gtcaggcggc aataccgacg acatgcgcgc ggcggtcaaa
    16981 ctgattgaag agaaaaaagt gcaggccgca aaagtggtaa cacatattct tgggctgaat
    17041 gccgcgggcg aaaccacgct tgaattgcct gccgtcggcg gcggcaaaaa gctggtgtat
    17101 accgggaaat acctgccgct gacgtcactc acgcagattc aggatcaagc actggcggcg
    17161 attctggcgc gtcatcaggg gatctggtcc ggtgaggcgg agcaatatct gctcactcat
    17221 gcagaggcaa tttcccatga ttaatcgcgc tacttaactc tgcatgtcgc tggctggtcg
    17281 ccgcggcaac ttcgcgacct gatgtgcggc tgattgctga agcggcccaa tgtgcacgag
    17341 aaggataaaa aaacatcagc gccttaaggg gcgctcaaat tggtggcaaa gtacacattg
    17401 tttatgccgg atgcggaata aagccttatt cggcctacat aatctcgaaa attcaatata
    17461 ttgcagttat ctggtaggcc tggtaagcgt agcgcatcag gcaatgctgt gtttgtcatc
    17521 agtctcagct cccggtgcaa tacgtgacat cgtgagccat gtcagtaagt ggttaaattc
    17581 gcagtggtag catgccttcc gtgatttctt actaaccgga gaagtaatgg aactgctttt
    17641 attgagtaac tcgacgctgc cgggtaaagc ctggctggaa catgcactgc cgctaattgc
    17701 tgaacagttg cagggtcgcc gctcagcggt gtttatccct ttcgctggcg taacgcagac
    17761 ctgggatgat tacacagcga aaacggctgc ggttctcgct ccgctgggtg tttctgtcac
    17821 cggtattcat agcgttgtcg atcccgttgc cgcgattgaa aatgctgaga tcgtgattgt
    17881 cggcggcggg aatactttcc agttgctgaa acagtgccgc gagcgcgggc tgctggcacc
    17941 aattactgac gtggttaaac gtggtgcgct gtatattggc tggagcgcag gcgctaacct
    18001 tgcttgccca accattcgta ccaccaacga tatgccgatt gtcgatccgc aaggtttcga
    18061 tgcgctaaat ctgttcccgt tgcaaatcaa cccgcacttc accaacgcgc tgccggaagg
    18121 ccataaaggt gaaacccgtg agcagcgtat tcgcgaactg ctggtcgtcg cgccagaact
    18181 gacgattatt ggtctaccgg aagataactg gatcacagtg agtaaaggtc acgctacgct
    18241 gggtggcccg aacaccacct atgtgtttaa ggccggtgaa gaagcggttc cgctggaagc
    18301 tggtcaccgt ttttaagtga attgccggat gcatgatccg gcatcttgtt catagtgcct
    18361 gatgcgacgc ttgacgcgtc ttatcaggcc tacaggattc acaatattaa tactcgtccc
    18421 gtccttcatc ttcatccgac tgttccagca cactgtaagc caccgaacaa aacagcgagt
    18481 tcaggcgctg catatctccc agtaagccta aatgtagcga actggtttca atgctctgca
    18541 cgttttgctg atgcaggcga tcgacgtggg cgtgggaata gcggcgatta agaatgcgaa
    18601 agcgatgctt actacggcgc aaacgacgag cgctggtgac atcgccagag aagaacaccg
    18661 acattgccag ctttaaatta ctgagcaatt gctcatagag cgcatccagt tccttcaacc
    18721 catcaagcga aaatgcccgc cgtgctgcca gtgatttgtc agcaatttcg ctgcccatgc
    18781 gctcgacgat atcggaggcc tgttcaaggt tgagcgacat ttcaataatc tccgcccagc
    18841 ggcgcgactc ttcctccgcc agctcttctt ttggcatccg cgccagatac agcttaatgg
    18901 cggtgtagag aacgttgata tcatctgcca gcttacgcag ctctttctcc tgccgtggct
    18961 caccgtgcat cactttattc aacccttcca tcatctgttc catggcgtcg ccaatgcgca
    19021 gggtttcgcg cgcggcgttc gccagagcaa gcgtgggcgt atccagcgcg ctgacatcca
    19081 gatgtttagg ccgtagctgg gtatccagtt ccggttcatc gcgaataatc gttttgcaaa
    19141 accgtgccat cgggtcaaca aatggcagca tgaccaggca acgtacaagg ttgtagaaga
    19201 cgtggaaata gatcaccagt tccgcttttg gcaatgacag cttccccatt gtctctgcca
    19261 gcaaatggac aaacggcagg ataatcaggc tacccaccag cttaaacagc agactaccca
    19321 gcgcgacacg gcgggctgcg gcattggcgg cactgttgtt gagcatcgcc agcaggccgg
    19381 aaccgaggtt agcaccaatc accagacaga gcgccacggg gaaggagata atgcctgcgg
    19441 cggtcagagc cgcggtcagc agtacagcag caaggctgga gtagctgata atggcgaaca
    19501 tcgcgccaat cagcgcatcc agcagaatat cgccggtcag cgaggcaaag atcacctgaa
    19561 cgccgtttgc ctgggtgatc ggcgttacgg cctgcacaat caactccagc gccagcaaaa
    19621 tcagcccaag accaataccg acgcggccca gttgcccggc gcgtgactgt ttgcgtccga
    19681 ggaaaaaaat cacgccgata aaaataagta acggtgacag ccaggataag tcgaaggtga
    19741 gaatacgcgc cattagcgcc gtcccgacat ctgcccccag cacaatgacc agagccggtg
    19801 cgagggctac cagatcctga gcgacgaacg aggtcaccag catggtggtg gcattactgc
    19861 tctgtaccag tgcggtaacg ccgatccccg cgcaaaaggc gagcggcttt ttttcgacgc
    19921 tccggctaag gacagtacgc aaacgcgcgc cgaagacgcg cattacgccg gttcgaacaa
    19981 tatgagtccc ccagaccagc agggcgacgg cagaaagcag gtgaagcagc gttaacacga
    20041 gttcaggttc tccttatcgt tatatgttcc tgaagcaatt gaatgaccaa aggaccggaa
    20101 acacgctgac tggcatcttt actgtgcctt ttgacattcg tttttccggc taagattatt
    20161 ccagcctgat tccagtataa gtgtttcata ttaataaaga gacagggcac tcattgagcg
    20221 ccctgtttgt tgtaaggaaa agtgacagat ttgtgaatca gtccgcgtca taccccagat
    20281 tcggtgccag ccagcgctca acatctgaga cgctcatacc tttacggcgg gcatagtctt
    20341 caacctgatc gcgctgaatt tgcgccacgg cgtagtactt gctgtccggg tggctgaaat
    20401 accaacccga aaccgatgca ccgggccaca tggcgaaaga ttctgtgagt ttcatgccgg
    20461 tatgtttttc cacttccagc agctcccaga tggtggcttt ttccgtatgt tccgggcagg
    20521 ccgggtagcc cggtgccgga cggatgccct ggtagttttc gcggatcagt tcttcgttgc
    20581 tgaggttctc gttcggcgca tagccccagt agactttacg cacacgctca tggagatact
    20641 ccgcaaaggc ttcggctaaa cggtcggcaa gcgctttcac catgattttg ttgtaatcat
    20701 cgtgctgcgc ttcaaaggca tcagccagtg cgtcctcttc cagcccgcca gtcacggcaa
    20761 atgcgccgat gtaatctgct ttaccagaaa gcttcggcgc aacgaagtca gcgagacagt
    20821 agttagcgaa tcccgttttt tcggtctgtt gacgcagatg gtggctgaca ttgatcacat
    20881 gggtgcgcgt ttcgtcacgg tagatttcaa tgtcatcgcc cacacggttt gccgggaaca
    20941 ggcccaccac gccacgccac gcggattcag cgttttctcg gcgcttaatt tatccagcat
    21001 gtcgttggcg tctttaaaca gccgctgcgc ctcaacgccc accacttcat cttccagaat
    21061 gcgcggatac ttcccggcca gcgaccaggt cataaagaac ggtgtccagt cgatgcaatt
    21121 acgcagagtt tcgatgctgg cttcaacttc ctgcacgccg agacggtgcg ccaccggcgg
    21181 cgtgtaagcc tgccagtcaa aagcgaagtc gttatcgcgc gccgcttcca gcgtgaccgg
    21241 tggtgtgcgc ggtttcttgc gcccgtgctg aatacgtacg gtttcgtact ccttgcgggt
    21301 acgagcgaca aaatcatcgc gctgagtatc ggaaagcagc gccgccacca caccaacggt
    21361 gcgcgaggca ttctgcacat acaccgtcgg gccgctgtag ttctgctcaa ttttcaccgc
    21421 cgtgtgcgct tttgaggtgg tcgcgccacc gatcagtaac ggaatggtga agccctgacg
    21481 ctccatctct ttcgccacgt taaccatctc gtccagcgac ggcgtgataa gccccgaaag
    21541 gccaatcaaa tcagcattca cttctttagc agtacggaga attttttccg caggcaccat
    21601 cacgccgaga tcgacaattt cgtagttgtt acattgcagc accacgccaa cgatattttt
    21661 accgatatcg tggacgtcgc ccttcacggt ggcgatcacc atcttgccgt tggttttgcc
    21721 ctgctctttg ctggcttcaa taaacggttc gagatacgcc accgcctgtt tcatgacgcg
    21781 cgccgatttg accacctgtg gcaggaacat tttgccttcg ccaaacagat cgccgaccac
    21841 gttcatgccg tccatcaacg ggccttcaat cacttcaatc gggcgcgtag cctgctggcg
    21901 ggcttcttcg gtatcctgct cgataaactc ggtaatgcct ttgaccagcg agtattccag
    21961 acgtttattc acttcccacg agcgccactc cgcctgctgg gcgttatcgg tgtcgtcggt
    22021 tttgctgccg cgatatttct cggcaagctc cagtaaacgc tcagtgccat cgtcacgacg
    22081 attaagaatc acatcttcca ccgcgtcgcg cagttcagcg ggtaggtcgt cgtaaatcgc
    22141 cagttgcccc gcgttgacga tccccatatc catgccattg cgaatagcgt agtagaggaa
    22201 cactgcgtga atggcttcgc gcaccggatc gttgccacgg aatgagaaag aaacgttaga
    22261 tacgccgccg gaaatcagcg cgtgcggcag ttcgcgtttg atgtcttcac acgcgccgat
    22321 aaagtcctgc gcgtagttgt tgtgctcttc aataccggtg gcgaccgcga agatgttcgg
    22381 gtcgaagatg atatcttctg gcgggaagcc aacctcttcg gtgaggattt tgtacgcccg
    22441 acggcaaatc tcgattttcc gtgcgcgagt atcggcctgt ccctgttcat caaaggccat
    22501 caccaccact gccgcaccgt agcgacgcaa caatttcgcg tgatggataa aggcgtcgac
    22561 gccctctttc atcgagattg agttaacaat gcctttgccc tgaatacact tcagcccttt
    22621 ttcgataacg tcccattttg aggagtcgat cataatcggc acgcgagcga tatccggttc
    22681 accggcaatc agattgagaa aacgcaccat cgccgcttcg gcatcgagca tcccttcatc
    22741 catgttgata tcgataatct gcgcgccgtt ttccacctgc tgacgcgcca catcctgcgc
    22801 ctcgctgtat ttctcttctt tgatcaggcg cttgaactta gcagagccgg tgacgttggt
    22861 gcgttcaccc acgttcacaa acaggctatc gtcgccaatg ttcagcggct ccaggccaga
    22921 caaacggcag gctacgggaa tttccggcag tttgcgcggc gctaatcctt ctactgcacg
    22981 actcatcgct gcaatatgtt gcggcgtggt cccgcagcaa ccaccgacga tattgagaaa
    23041 gcccgcttgc gcccattcac gtatctgttt tgccatcgtg tcggcgtcga gatcgtattc
    23101 accaaaggcg ttaggcagcc cggcgttcgg gtgggcggtg acgtagcatt ccgcaatccg
    23161 tgatagttcc tgcacgtact ggcgcagttc atcgggcccc agcgcacagt tcaggccaaa
    23221 ggttagcgct tcggcgtggc gcaatgagtt gtaaaatgct tcggtggtct gcccggagag
    23281 cgtgcgcccg gaggcgtcgg tgatggtgcc ggagatcata atcggcagct caacgcccag
    23341 cgcttcaaac tccgttttca ccgcaaaaac cgccgctttg gcgttaaggg tgtcgaaaac
    23401 ggtttcaatc aggatcagat ccgcaccacc ttccaccagc gctttggtgg attcgcgata
    23461 ggccgccacc agctggtcaa aagtgatgtt gcggaatgcc ggatcgttga cgtccggaga
    23521 aatagacgcc gtgcggttgg ttgggccgag aacaccggca acgtagcgcg gtttctccgg
    23581 cgtgcgcgcg gtccactcgt cagcacaggc gcgcgccagt ttcgccgccg caaagttgat
    23641 ttccgccgac agggattcca tctggtaatc cgccatcgca atggtcgtgg agttgaaggt
    23701 gttggtttcg atgatatccg cgcccgcttc aaagtaggcg ttgtggatag cggcgatcac
    23761 ttccggtttg ctgagcacca gcaggtcgtt gttgcctttg agatcgcatg gccagtcggc
    23821 aaagcgttca ccacgaaaat cggcttcgtt cagtcgataa ctctggatca tggtgcccat
    23881 accgccgtcc agcaccagaa tacgttcatt taactgcgca cgcagttgtt ccactttgct
    23941 gctcacactt gctcccgaca cgctcaaccc agatttaata aaaattcaac aaaccatact
    24001 ggcataaacg catctgtggt aaaagcgacc accacgcaac atgagatttg ttcaacatta
    24061 actcatcgga tcagttcagc aactattgca ttagctaaca ataaaaatga aaatgattcc
    24121 cacgatacag aaaaaggaga ctgtcatggt cgcacccatt cccgcgaaac gcggcagaaa
    24181 acccgccgtt gccaccgcac cagcgactgg acaggttcag tctttaacgc gtggcctgaa
    24241 attactggag tggattgccg aatccaatgg cagtgtggca ctcacggaac tggcgcaaca
    24301 agccgggtta cccaattcca cgacccaccg cctgctaacc acaatgcaac agcagggttt
    24361 cgtgcgtcag gttggcgaac tgggacactg ggcaatcggc gcacatgcct ttatggtcgg
    24421 cagcagcttt ctccagagtc gtaatttgtt agcaattgtt caccctatcc tgcgcaatct
    24481 aatggaagag tctggcgaaa cggtcaatat ggcggtgctt gatcaaagcg atcacgaagc
    24541 gattattatc gaccaggtac agtgtacgca tctgatgcga atgtccgcgc ctatcggcgg
    24601 taaattgccg atgcacgctt ccggtgcagg taaagccttt ttagcccaac tgagcgaaga
    24661 acaggtgacg aagctgctgc accgcaaagg gttacatgcc tatacccacg caacgctggt
    24721 gtctcctgtg catttaaaag aagatctcgc ccaaacgcgc aaacggggtt attcatttga
    24781 cgatgaggaa catgcgctgg ggctacgttg ccttgcagcg tgtattttcg atgagcaccg
    24841 tgaaccgttt gccgcaattt ctatttccgg accgatttca cgtattaccg atgaccgcgt
    24901 gaccgagttt ggcgcaatgg tgattaaagc ggcgaaggaa gtgacactgg cgtacggtgg
    24961 aatgcgctga ctttttctgg cgggcagagg caatattctg cccatcata
//
LOCUS       DAJTMC010000045        24875 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.045, whole genome shotgun sequence.
ACCESSION   DAJTMC010000045 DAJTMC010000000
VERSION     DAJTMC010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24875
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.045"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(<1..337)
                     /locus_tag="OO130_001649"
     CDS             complement(<1..337)
                     /locus_tag="OO130_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5365104.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAA"
     gene            complement(453..1004)
                     /gene="maa"
                     /locus_tag="OO130_001650"
     CDS             complement(453..1004)
                     /gene="maa"
                     /locus_tag="OO130_001650"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HCR5365105.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKGVPDNVVVGGNPARIIKKL"
     gene            complement(1176..1394)
                     /gene="hha"
                     /locus_tag="OO130_001651"
     CDS             complement(1176..1394)
                     /gene="hha"
                     /locus_tag="OO130_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HCR5365106.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(1420..1794)
                     /gene="tomB"
                     /locus_tag="OO130_001652"
     CDS             complement(1420..1794)
                     /gene="tomB"
                     /locus_tag="OO130_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HCR5365107.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNYGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(2340..5489)
                     /locus_tag="OO130_001653"
     CDS             complement(2340..5489)
                     /locus_tag="OO130_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HCR5365108.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(5512..6705)
                     /gene="acrA"
                     /locus_tag="OO130_001654"
     CDS             complement(5512..6705)
                     /gene="acrA"
                     /locus_tag="OO130_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HCR5365109.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDNKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTAPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            6847..7494
                     /gene="acrR"
                     /locus_tag="OO130_001655"
     CDS             6847..7494
                     /gene="acrR"
                     /locus_tag="OO130_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HCR5365110.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            7622..10984
                     /gene="mscK"
                     /locus_tag="OO130_001656"
     CDS             7622..10984
                     /gene="mscK"
                     /locus_tag="OO130_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HCR5365111.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLELQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIVGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYIDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(11196..11357)
                     /gene="rsmS"
                     /locus_tag="OO130_001657"
     CDS             complement(11196..11357)
                     /gene="rsmS"
                     /locus_tag="OO130_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HCR5365112.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LTRDDEAEK"
     gene            complement(11371..11898)
                     /gene="priC"
                     /locus_tag="OO130_001658"
     CDS             complement(11371..11898)
                     /gene="priC"
                     /locus_tag="OO130_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HCR5365113.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVAQFATLSARFDRHLFQTRTT
                     TLQACLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            11968..12345
                     /locus_tag="OO130_001659"
     CDS             11968..12345
                     /locus_tag="OO130_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HCR5365114.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            12498..13049
                     /gene="apt"
                     /locus_tag="OO130_001660"
     CDS             12498..13049
                     /gene="apt"
                     /locus_tag="OO130_001660"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HCR5365115.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            13178..15049
                     /gene="dnaX"
                     /locus_tag="OO130_001661"
     CDS             13178..15049
                     /gene="dnaX"
                     /locus_tag="OO130_001661"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HCR5365116.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTQVPPQ
                     PQSAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVPSALEKAP
                     AKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELAAKLAAEAIERDPWAARVS
                     QLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGAQQKLAEALSTLKGSTVEL
                     TIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQTLRRFFDAELDEESIRPI"
     misc_feature    14442..14506
                     /gene="dnaX"
                     /locus_tag="OO130_001661"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            15102..15431
                     /locus_tag="OO130_001662"
     CDS             15102..15431
                     /locus_tag="OO130_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HCR5365117.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            15431..16036
                     /gene="recR"
                     /locus_tag="OO130_001663"
     CDS             15431..16036
                     /gene="recR"
                     /locus_tag="OO130_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HCR5365118.1"
                     /translation="MQTSPLLTQLMEALRCLSGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            16146..18020
                     /gene="htpG"
                     /locus_tag="OO130_001664"
     CDS             16146..18020
                     /gene="htpG"
                     /locus_tag="OO130_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HCR5365119.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTHTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            18201..18845
                     /gene="adk"
                     /locus_tag="OO130_001665"
     CDS             18201..18845
                     /gene="adk"
                     /locus_tag="OO130_001665"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HCR5365120.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRADLEKILG"
     gene            18977..19939
                     /gene="hemH"
                     /locus_tag="OO130_001666"
     CDS             18977..19939
                     /gene="hemH"
                     /locus_tag="OO130_001666"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HCR5365121.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEGGSPLMVYSRQQQQALAQRLPETPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEYIEMMANLVAAYR"
     gene            complement(19936..20895)
                     /gene="aes"
                     /locus_tag="OO130_001667"
     CDS             complement(19936..20895)
                     /gene="aes"
                     /locus_tag="OO130_001667"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HCR5365122.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPSWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYPLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            21047..22351
                     /gene="gsk"
                     /locus_tag="OO130_001668"
     CDS             21047..22351
                     /gene="gsk"
                     /locus_tag="OO130_001668"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HCR5365123.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(22484..24160)
                     /locus_tag="OO130_001669"
     CDS             complement(22484..24160)
                     /locus_tag="OO130_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HCR5365124.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKAIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSLAAFFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVAYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(24398..>24875)
                     /gene="fsr"
                     /locus_tag="OO130_001670"
     CDS             complement(24398..>24875)
                     /gene="fsr"
                     /locus_tag="OO130_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HCR5365125.1"
                     /translation="YYTFYLMQKFGLSIQNAQLHLFAFLFAVAAGTVIGGPVGDKIGR
                     KYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFSAILVYAQELLPGRIGM
                     VSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLLGMLTIFLPDNRHKD"
BASE COUNT         6215 a         6483 c         6390 g         5787 t
ORIGIN      
        1 gcgcggcagg tacaaagcgc gtgttgtacc attcgccgtt atagcaaacg cggaaatcca
       61 gcacgtcgat atcttcatag cggtagcgtg ggtagacatc gaaaaagaag aaaccattaa
      121 gcaggataaa taatacaacc agtagccaga cagagcctga cagcgtttcg gactgcaaca
      181 tcaccgccag ggtggcaaaa aaagcaacgt acataccgat aaacagcccc ggatgtttac
      241 ggataaaact gatactaaag cggggtttgt tgtcgcgttt ttcgcgggta ttcagagact
      301 caatcgtttc ggtcagcagg cgttgtattt cggtcatcgt ttgccttcgt ggtatctgca
      361 actttccagg gaacacccta ttttaacgtg ggaatagtag aggggaagta acagatctac
      421 cacaattttg cataacagtt gcgaaaaacc gattacaatt ttttaattat tctggctgga
      481 ttaccgccca cgacaacgtt gtccgggaca ccttttgtga caactgcacc tgaagctacc
      541 acgacgttat caccaatggt cacaccaggg ttaatgaccg cgcgtccgcc aatccagacg
      601 ttattaccga tggtgacggg tttccccagt tcagcaccgc tattacgtgc tacagggtcg
      661 atgggatgtg ttgccgtgta gatatgaacg cctggtgcca acatacagtt atcaccgatg
      721 cgaatagggc agacatcaag catcacgcaa tcgaagttgg cgaaaaaatt attgccgaga
      781 aaaatgttat agccataatc acagcgaaac gttggctcaa tataagcctc tgtcacctga
      841 ccgaataaat cagcgagaat ttgctggcgt aatgtgtgct cttccgccag ggaatgattg
      901 tatcggtgaa taagctgacg agcgcgcagg cgatcgcgag ataacgtctc atctgccgag
      961 cgatacaact caccagcaat catcttttct ttttctgtgc tcattactga cctccggttg
     1021 ctgaaaatgc ggcaacacag taagacgaaa agtcagtgaa cagaacgttc ccattacatc
     1081 tgtgtgataa agatcacata gggtatgtct tcatggtgaa aaagtataaa attcttaata
     1141 aacagccggt tatagctccg aaagcgaatt atttattagc gaataaattt ccatactgag
     1201 gaagggatct tgtcgtatag tttattcatg gtcaattcgg cgaggcggtg atctgcggct
     1261 gagtaaaata ccgccagttc attatctgat aattcgtatt tatttttctc gataacacgc
     1321 tccagcgtgt caattgtctg gcaacgacgt aaacgcatta aataatcggt tttcgttaaa
     1381 ggtttttcgg acatacttct acccatggtt gtaataattc taacaagata aactcgcagg
     1441 attctctttc gtcgcattga caaaacaacg gaatagtcga ttacctgact tccgccattt
     1501 ctgaagatcc tgcatattaa taccataact actgaacaac ataaaggtgt catccagata
     1561 ttcgtcgatc tgctcaatga gcttattgtc ttcattatac ttaattttgt aattaagtgc
     1621 gaaggtcgca atatgctcaa tcagttcatt caactggagg ttgatcgccg aggttgggtc
     1681 gttaacccag ccataattgc tttcttcaag gtttgcaagg cagtcatgat acagggtttc
     1741 acagagaaac ttaagctgtg cgatatcatg tcttttgggt gagtattcat ccataacgcg
     1801 tccccttctt agcggttgaa ctaacggaca cctttcggga tggaaaaaac ttactgacct
     1861 ggacttgccc tctcgtttgt tagcttaact ataagccact ctttgcaggt attcatcgca
     1921 ttattacgaa aaattacaat taggaaggaa tcgggacagg actcactttt tcatactagc
     1981 actaacccgc agcaggtaaa agcagtttgc tcgaactgtt cataacattt caggattatc
     2041 gccagatgaa attggtgaca ctaataccag gattgctctg aatatgacgt aataaccgag
     2101 gaatgaataa agaattaccg caaataatta agaatagcct ctaaatgatt atggattatt
     2161 aaatctatta gcgctactta atataaatta actaacaatc agtatttttt atgaatttta
     2221 tccgtggtta atactggttt tcgtatgaga tcctgagttg gtggttcaat tactccttaa
     2281 tgttcgtagg ttatgcataa aaaaggccgc ttgcgcggcc ttagtgatta cacgttgtat
     2341 caatgatgat cgacagtatg gctgtgctcg atatcttcat tcttgcggct aaagcggcgg
     2401 cgaaccacca caaagaatac cggaacgaag aagattgcca gtaccgttgc ggtcaccatc
     2461 ccgcccatta caccggtacc tactgcgttc tgcgcgccgg aaccagcacc agtactgata
     2521 accagcggca taacgccgag gataaacgcc agcgaggtca tcaggatcgg acgtaaacgc
     2581 atccgcactg catcaagcgt cgcttcaatc agacctttac cttctttatc catcaagtct
     2641 ttggcgaatt cgacgataag tatcgcgttc ttcgccgaca acccaatggt tgtgagcagg
     2701 cctacttgga agtaaacgtc attggtcagg ccacggaagg tggcagccag caacgcaccg
     2761 ataaccccca gcggaacgac cagcataacg gagaacggaa tcgaccagct ctcgtacagc
     2821 gccgctagac acaggaatac gacaatcaac gaaatcgcgt acagtgaagg tgcctggttg
     2881 ccggagaggc gttcctgata ggacatcccc gtccagtcat agccaacacc ggtaggcagt
     2941 ttgctcgcca gttgttccat tagctccatt gcttcaccgg tacttttacc cggtgccgcc
     3001 tggcctaaga tttccatgga tggcaggcca ttgtaacgtt ccagacgcgg cgaaccgtac
     3061 tcccaacgag aagaggagaa tgccgagaac ggcaccatct gaccatcagc agcacgaaca
     3121 taccagtcgc cgatatcatc cggcagcata cggtatttcg cttctgacat gacgtaaact
     3181 ttcttcacac gaccgcggtc gataaagtcg ttcacatagc taccgcccca tgcagcgccc
     3241 agagtggtgt taatgtcgtt gatagaaaca cccagcgcct gcgctttttc ctggtcgata
     3301 tcaatcttaa actgcggggt atcttccaga ccgtttggac gtacgctggt caacatatca
     3361 ggatgctttg ctgcttctgc aagcagctgg ttacgcgcct gagtcagttt ttcgtgacca
     3421 aggccagcct ggtcaatcag ctcaaagtca aagccggttg cagtacccag ttccacgatt
     3481 gcgggcaggt taaaggcgaa aaccatcgca tctttgattt gcgagaaagc gcgtgttgca
     3541 cgcatggtaa tcgcttcaac tttgttttct tcgcccggac gatcggccca gtccttcaag
     3601 gaaacgaacg caataccggt attctgacca cgtcccgcaa agccgaagcc gttaacggcg
     3661 aacaccgact caacgttgtt cttttctttg gtcagatagt aatgcgttac ctcattgagc
     3721 actttctgcg tacgttcctg cgttgcacct gctggcagct gaaccatggt cataaatacg
     3781 ccctggtcct catctggcaa gaaggagctt ggcagacgca cgaacagata ggccatgccg
     3841 accacgatga tcagatacag caccaggtaa cgccccgtac tgcgcagaat accgcctacg
     3901 ctgtcggtgt agtggtgcgt gctcttctcg aacatgcggt taaaccagcc gaagaagcct
     3961 tttttacctt ccccgtgatc gcctttggca atcggtttca gcatggtggc acaaagagcc
     4021 ggagtcagga tcaacgctac cagtaccgac agcgccattg ctgaaacaat ggtaatagag
     4081 aactgacgat agatagcacc agtagaaccg ccaaagaagg ccatcggtac gaataccgct
     4141 gacagtacca tcgcgatacc gaccagagcg ccctgaatct gccccatcga cttacgggta
     4201 gcttcttttg gcggcaaacc ttcttccgcc ataacacgct ccacgttttc taccacaacg
     4261 atagcgtcat ccaccaacaa gccgatggcg agcaccatcc cgaacattgt tagcgtgttt
     4321 atcgagaagc caaaggcggc aaggacggca aaggtcccga gcaataccac cggtacggca
     4381 atggtcggaa tcaacgtcgc gcggaagttc tgcaggaaca gatacataac caggaacacg
     4441 aggatgatcg cttcgaccag cgttttaacc acttcgtgaa tagagatttt cacgaacggc
     4501 gtggtgtcgt acgggtaaac aattttcaga cccgacggga agaacggttc catcttcgcc
     4561 agttcagcac ggattgccgc agcggtatcc agcgcatttg caccggtcgc cagcttgatc
     4621 cccagaccgg aagccggttg gccgttaaac tctgcgatga tgtcgtagtt ctcaccaccc
     4681 agctcaatct tcgccacatc acgcagcagg acgcgggaac catcctgatt cactttcagc
     4741 aggattttgc cgaactcttc agtagaggtc agacgcgtct gagcaataat agaggcgtta
     4801 agctgttggc ctttcaccgg cggcgtacca ccgagctgac ccgccgcaac ctgggcgttc
     4861 tgcgctttga tggcggtaat gacatcaacc ggcgttagct ggaatttgtt cagctcattc
     4921 gggttcatcc agatacgcat cgcgtactgt gaaccgaaca actgaacatc acccacgccc
     4981 gacgtacggc tgatggcatc tttcatattc gccgctacgt agtcagagat atcctcctgc
     5041 gtcatggtgc catcggtgtt gataacgccg acaaccatca ggaagctgct ggatgatttc
     5101 tcaacgctca ccccttgctg ctgaacttct tgcggcagca acggcatcgc cagctgcagt
     5161 ttgttctgca cctgaacctg cgcgatatcc gcatcagtac cagactcaaa ggtcagggtg
     5221 atctgcacgg tccccgtgga gtcactgtta gaggacatgt acatcaggtt atcgataccg
     5281 ttcatattct gttcgataac ctgtgtcacc gtgtcctgca ctgttttcgc atcagcgccg
     5341 gggtaggagg cggagatcgt tactgccggc ggtgcaatcg taggatattg cgccaccggc
     5401 agtttgagga tcgccagccc ccctgccaac atgatgataa tggcgatcac ccacgcaaaa
     5461 atcgggcgat cgataaagaa attaggcatg tcttaacggc tcctgtttaa gttaagactt
     5521 ggactgttca ggctgagcac cgcttgcggc ttgctggtta ttatcagcgg taacttcttg
     5581 tgcttttacc tggacaccag gacgcacttt ctgcagccca cttattacta cgcgatcgcc
     5641 tgctttcaga ccttctgtca ccagccactt atcgccaata gcctggcttg caacgatcgg
     5701 acgggtttcc actttgtcat ccgcgccaac taccagtacg gtggcatcac cacgcggcgt
     5761 acgggttacg ccctgttgcg ggactaaaat agcgtttgga ttaagccctt cttccagacg
     5821 tgcgcgcacg aacatacccg gcagcagagt gtgatccggg ttcgggaaga tagcgcgtag
     5881 ggtgatagac ccagtggtct gatcaacggt aacgtcagag aattccagcg taccgtcctg
     5941 cgggaactta atgccgtcac tggtgatcag cgacactttg gctttgccgt tctcttgttt
     6001 cagcgtgcca ttcgccagtt cctgtttcag gcgcaggaag tcgttgctgg actgggtcac
     6061 atcaacgtag atcggatcaa gttgctgcac ggttgccagc gcagtcgcct gaccgttctg
     6121 taccaatgcg ccttccgtca cgttcgactt accaatgcga ccgctaatcg gagcggtgac
     6181 tttggtgtaa gccagattga tccgcgcagt ttcaacggca gctttcgccg cagttaccgc
     6241 agcattcgcc tgttgcgcat cagccagagc ctgatcgtac tcttgcttac tgatgtactg
     6301 agtaccgagc agtttctgat aacgattcac cgtcaattgc gcgatattgg ctgcagcctg
     6361 ggctttcgcc agatcacctt tcgcactgtc gtatgtcgcc tgataggtcg caggatcaat
     6421 ctgatagaga gagacaccag cttcgatgtc gctaccttct ttgaaattac gcttcaggat
     6481 aatcccgcta acttgaggac gaacttctgc gatccggtag gcactggtgc gacccggaag
     6541 ctcggttgtg atctgcagag gttcagtttt gactgttact acgccaacgg cgggcatctg
     6601 ctggccacct tgttgggcct gtttgttgtc acatcctgtt agggctaagc tgcctgagag
     6661 catcagaacg accgccagag gcgtaaaccc tctgtttttg ttcatatgta aacctcgagt
     6721 gtccgatttc aaattggtca atggtcaaaa gttaataaac ccattgctgc gtttatatta
     6781 tcgtcgtgct atggtacata cattcacaaa tgtatgtaaa tctaacgcct gtaaattcac
     6841 gaacatatgg cacgaaaaac caaacaagaa gcgcaagaaa cgcgccaaca catcctcgat
     6901 gtggctctac gtcttttctc acagcagggg gtatcatcca cctcgctggg tgagattgca
     6961 aaagcagctg gcgttacgcg cggtgcaatc tactggcatt ttaaagacaa gtcggatttg
     7021 ttcagtgaga tctgggaact gtcagaatcc aatattggtg aactagagct tgagtatcag
     7081 gcaaaattcc ctggcgatcc actctcagta ttaagagaga tattaattca tgttcttgaa
     7141 tccacggtga cagaagaacg acgtcgatta ttgatggaga ttatattcca caaatgcgaa
     7201 tttgtcggag aaatggctgt tgtgcaacag gcacaacgta atctctgtct ggaaagttat
     7261 gaccgtatag aacaaacgtt aaaacattgt attgaagcga aaatgttgcc tgcggattta
     7321 atgacgcgtc gggcagcaat tattatgcgc ggctatattt ccggcctgat ggaaaactgg
     7381 ctctttgccc cgcaatcttt tgatcttaaa aaagaagccc gcgattacgt cgccatctta
     7441 ctggagatgt atctcctgtg ccccacgctt cgtaatcctg ccactaacga ataaccctga
     7501 atctgactcc aggatttttc ctggacattt tcgtcgttgc tattctggtt cactgcgtcg
     7561 tgatattctt gcggtttgac tttttcaggt cgttcttcag gttcagaaac cttcattcat
     7621 catgactatg ttccagtatt acaaacgatc acggcatttt gttttttcag catttattgc
     7681 ttttgttttt gtcttgttat gccagaacac ggcgtttgcg cgggcgtcat cgaatggtga
     7741 tctgccgaca aaagcggacc tgcaggcgca acttgactca ctaaataaac aaaaagatct
     7801 ttctgctcag gacaaactgg agctgcagga tctgacagat acattagcca ccctcgataa
     7861 aatcgatcgc ataaaagaag agacagttca gctacggcaa aaagtcgctg aagcgccgga
     7921 aaaaatgcgc caggcgaccg cggcgttaac agcacttagc gatgtcgata acgacgaaga
     7981 aacgcgcaaa attctgagta cgctttcgtt gcgccagctg gaaactcgcg ttgcccaggc
     8041 gctggacgat ttgcaaaacg cacaaaacga tctggcgtct tataacagcc agctggtttc
     8101 gttacagacg cagcccgaac gcgtgcaaaa tgcgatgtat aacgcttcgc agcagctgca
     8161 acaaattcgc agtcgtctgg atgggactga tgtcggcgag actgccttac gtcccagcca
     8221 gaaagtgtta atgcaggctc agcaggcgtt gctgaatgcg gagattgacc agcagcgtaa
     8281 aagcctggaa gggaacaccg tcttgcagga taccttgcaa aagcaacgtg attacgtgac
     8341 ggcgaacagc gcccgtctgg agcaccagtt gcagctgttg caagaagcgg taaacagcaa
     8401 gcgcctgact ttaactgaaa aaacggcgca ggaagccgtc tccccggatg aagccgcgcg
     8461 tattcaggct aatccgctgg tgaaacagga actggaaatt aaccagcagt taagtcagcg
     8521 tctgattacc gcgactgaaa acggtaatca gttgatgcag caaaacatta aagtcaaaaa
     8581 ctggctggag cgggcgctgc aatcggaacg caatattaaa gagcagattg ccgtcctgaa
     8641 gggcagcctg ctgttgtctc gtatccttta ccagcaacaa caaacgctgc cctcggcgga
     8701 tgaactggaa aacatgacca accgcatcgc ggatttgcgt ctcgaacagt ttgaagttaa
     8761 ccagcagcgt gatgcactct tccagagcga tgcgttcgtc aacaaactgg aagaaggtca
     8821 caccaacgaa gtcaacagcg aagttcacga tgcgttattg caagtggttg atatgcgtcg
     8881 cgaattgctg gatcaactca acaaacagtt gggtaaccag ctgatgatgg ccattaacct
     8941 gcaaatcaac cagcagcagt taatgagcgt atcgaaaaac ctgaaatcca tcctgactca
     9001 gcaaatcttt tgggtgaaca gtaaccgtcc aatggactgg gactggatca aagcgttccc
     9061 gcaaagcctg aaagatgaat ttaagtcgat gaaaatcacg gtgaactggg aaaaagcctg
     9121 gcctgccgtt tttatcgctt tcctcgccgg tttgccgctg ctgttgattg tcgggctgat
     9181 ccactggcgt ctgggctggc tgaaagcgta tcaacaaaaa ctggcttccg ctgtgggttc
     9241 cctgcgtaac gatagccagc tcaacacacc aaaagcgatc cttatcgacc tgatccgcgc
     9301 gctgccggtg tgcctgatta ttctcgcggt tggcttgatt ctgttgacca tgcagctcaa
     9361 catcagcgaa ctgctatggt cgttcagcaa aaaactggcg attttctggc tggtgtttgg
     9421 cctgtgctgg aaggtactgg agaagaacgg cgttgccgtg cgtcacttcg gcatgccgga
     9481 acagcagacc agccactggc gtcggcaaat tgtccgcatc agtctcgcat tgctgcctat
     9541 ccatttctgg tctgtggtgg cagaactttc cccgctgcat ctgatggatg atgtgctggg
     9601 gcaagcgatg attttcttca acctgctgct gattgccttc ctggtatggc caatgtgccg
     9661 cgaaagctgg cgtgataaag agtcgcacac catgcgactg gtcaccatta ccgtgctgtc
     9721 gattatcccg attgcattga tggtgctgac tgccacgggt tatttctaca ccacgctgcg
     9781 cctctccggg cgttggattg aaaccgttta tctggtgatc atctggaact tgctctacca
     9841 gacggtattg cgcggcttaa gcgtagcggc gcggcgtatc gcctggcgtc gtgcgctggc
     9901 gcgtcggcag aatctggtga aagagggcgc agaaggtgcc gaaccgccgg aagaacccac
     9961 cattgcactg gaacaggtta accagcagac gctgcgtatt accatgttgc tgatgtttgc
    10021 gctgttcggt gtcatgttct gggcaatttg gtccgatttg atcaccgtgt tcagctatat
    10081 cgacagcatt acgctctggc attacaacgg tactgaagct ggcgctgcgg tggtgaaaaa
    10141 cgtcaccatg ggcagtctgt tgtttgcgat tatcgcctca atggtggcct gggcgttgat
    10201 tcgcaacctg cctggtttgc tggaagtgct ggtgctctcg cgactgaata tgcgccaggg
    10261 cgcgtcgtat gccattacta ccatccttaa ctacatcatt attgccgttg gtgcgatgac
    10321 ggtgttcgga tcgctgggcg tctcttggga taaactccag tggctggcgg cagcattatc
    10381 cgtaggtctt ggttttggtt tacaagaaat tttcggtaac ttcgtctccg gtttgatcat
    10441 tctgttcgaa cgtccggtgc gtattggcga tacggtaacc attggtagct tctcggggac
    10501 ggtaagtaag atccgtattc gtgcgacaac gattaccgat ttcgatcgca aagaagtgat
    10561 catcccgaac aaagcgtttg ttaccgagcg tctgatcaac tggtcgttga ctgacactac
    10621 tacgcgtctg gtgatccgtc tcggcgtggc ctatggctcc gatctggaaa aagtgcgtaa
    10681 agtgttactg aaggcggcga ctgagcaccc aagggtgatg cacgaaccaa tgccggaagt
    10741 cttctttacg gcgtttggtg ccagcacgtt ggatcatgag ctgcgtctgt atgtgcgtga
    10801 actgcgtgac cgtagtcgta ctgtcgatga gctgaaccgt actatcgatc agctgtgccg
    10861 tgaaaacgac atcaacattg cctttaacca gcttgaagtg catctgcaca acgagaaggg
    10921 cgatgaggtg acggaagtaa aacgcgacta caaaggcgat gacccgacgc cagcggtagg
    10981 gtaataacca aagggggcga catttagttg ccccgagatt actgacaaag tgcgcgttgt
    11041 tcatgccgga tgcggcgtga ccgccttatc cggcctacga aaccgcaaga attcaatgta
    11101 ttgcaggagc ggtgtaggcc tgataagcgt agcgcatcag gcagttttgc gtttgcccac
    11161 aatcttaggg gacatttagc ggccccattt atttctcact tttccgcctc atcatcgcgc
    11221 gttaatttct tttcataatc gcgttttaca atatccagcg cgcgcagcac ggtgcgggct
    11281 gggatctgat tttcttccag cagcacaatc aaatcgacag ccagtttgac atcgtcaggg
    11341 gcattttcca gtgacatact ctctccattg ctagcgggtt aagcgcgcta gcctgttttc
    11401 gattttttcc agcgcatggc ggcagcgtgc caggcgcgct tcataggctt ccacttcacg
    11461 atgcagcgtt tgctgttcca cgagatcggt cacccgcgcc aggcgggctc tgcgttcggc
    11521 aaccatctca cgtagccgcc gctcaaaatc ctgatgctga atacgtttac gctgccagcg
    11581 ggcaattttc ggtggcgcac tgtcccactc gcgcaatgac caggcggagg cttcacgcgc
    11641 gatggcttcc agttgtgccg ccagatgttc cgccagccag gccacttgcg gcagctgttg
    11701 ctgctcaact gcatgacgaa gcgcagccag attatcgcca gcctcatcga gacaagcctg
    11761 cagtgttgtc gtacgggttt gaaaaaggtg tctgtcaaaa cgggcactta gcgtggcaaa
    11821 ttgcgctacc ggggcacaac gctgacgcag cgtagcgagc tgtccttcca gtttttccag
    11881 cagcagggcg gttttcacga taagacctca atgaaaatga taattgttat gctaaagtag
    11941 ccactctgta agctgacaac actcaatatg caacgaatca ttttaatcat cattggctgg
    12001 ctggcggtag tgctgggtac gctgggcgtg gtattaccgg tattaccgac gacgccgttt
    12061 atcctgctgg cggcctggtg ctttgcccgt tcttccccgc gctttcacgc ctggttgctg
    12121 taccgctcat ggtttggcag ctatctacgt ttctggcaga aacatcatgc gatgccgcgc
    12181 ggcgtcaaac cgcgggcgat tttgcttatt ttgctcacgt ttgccatttc tctgtggttc
    12241 gtccagatgc catgggtgcg catcatgttg ctggtaattc tcgcctgttt gcttttctat
    12301 atgtggcgaa ttccggtgat tgatgaaaag caagaaaagc actgaagcac aacaatcgca
    12361 gttgcaatta ttgcgtacag ccagtacatt ctggcgtttt cgagcacagg cgcaggcggt
    12421 caaaggttaa acaactgtta cttttgatac gtttaaaacg cgccgtgagt accaccgtaa
    12481 caagcaggca tacacttatg accgcgactg cacagcagct tgagtatctc aaaaatagca
    12541 tcaaaagcat tcaggactac ccaaaacccg gcattctttt ccgcgatgtc accagcttac
    12601 tggaagaccc gaaagcttac gctctcagca tcgacttgct ggttgagcgt tacaaaaatg
    12661 cgggcattac caaagttgtc ggcaccgaag cgcgtggctt cttgtttggc gctccggtag
    12721 ctctgggtct gggcgttggc tttgtaccgg tccgtaaacc gggcaaactg ccgcgtgaaa
    12781 ccatcagtga aacttacgac ctggaatacg gcaccgatca gctggagatc cacgttgatg
    12841 ccatcaaacc gggcgacaaa gttctggtgg tggacgatct gctggcaacc ggcggcacta
    12901 tcgaagcgac cgttaaactg atccgtcgtc tgggtggtga agtggctgac gctgcgttca
    12961 ttatcaacct gttcgatctc ggcggcgaac agcgtctcga aaaacagggc attaccagct
    13021 acagccttgt cccgttcccg ggccattaat tatcgccagt cttgtgctgc ccacgctacg
    13081 gacagcacaa gatgtgcatt cagcctcgcc gttctgacgg ggctgtgtta gcattacccc
    13141 ttcgtgaatc caccttccag cgtttcagag cctgccaatg agttatcagg tcttagcccg
    13201 aaaatggcgc ccacaaacct ttgctgacgt cgtcggccag gaacatgtgc tgaccgcact
    13261 ggcgaacggc ttgtcgttag ggcgtattca tcatgcttat cttttttccg gtacccgtgg
    13321 cgtcggaaaa acctctatcg cccgactgct ggcgaagggg ctaaactgcg aaaccggcat
    13381 taccgcgacg ccgtgcggcg tgtgcgataa ctgtcgtgaa atcgagcagg ggcgctttgt
    13441 cgatctgatt gaaatcgacg ccgcctcgcg caccaaagtt gaagataccc gcgacctgct
    13501 ggataacgtc cagtacgctc cggcgcgtgg tcgtttcaaa gtttatctga tcgacgaagt
    13561 gcatatgctg tcgcgccaca gctttaacgc actgttaaaa acccttgaag agccgccgga
    13621 gcacgttaag tttctgctgg cgacgaccga tccacagaaa ttgccggtga cgattttgtc
    13681 gcgctgtttg caatttcatc tcaaggcgct ggatgtcgag caaattcgcc atcaacttga
    13741 gcacatcctc aacgaagaac atatcgctca cgagccgcgg gcgttgcaat tgctggcgcg
    13801 cgccgctgaa ggcagcctgc gagatgcctt aagtctgacc gaccaggcga ttgccagcgg
    13861 tgacggccag gtttcaaccc aggcagtcag tgcgatgctg ggtacgcttg atgacgatca
    13921 ggcgctgtcg ctggttgaag cgatggtcga ggccaacggc gagcgcgtaa tggcgctgat
    13981 taatgaagcc gctgcccgtg gtatcgagtg ggaagcgttg ctggtggaaa tgctcggcct
    14041 gttgcatcgt attgcgatgg tacaactttc gcctgctgca cttggcaacg acatggccgc
    14101 cattgagctg cggatgcgtg aactggcgcg caccataccg ccgacggata ttcagcttta
    14161 ctatcagacg ctgttgattg gtcgcaaaga attaccgtat gcgccggacc gccgcatggg
    14221 cgttgagatg acgctgctgc gcgcgctggc gttccatcca cgtatgcctc tgcctgagcc
    14281 agaagtgccg cgccagtcct ttgcacccgt cgcgccaacg gcagtaatga cgccaaccca
    14341 ggtgccgccg caaccgcaat cagcgccgca gcaggcaccg actgtaccgc tcccggaaac
    14401 caccagccag gtgctggcgg cgcgccagca gttgcagcgc gtgcagggag caaccaaagc
    14461 aaaaaagagt gaaccggcag ccgctacccg cgcgcgtccg gtgccatcgg cgctggaaaa
    14521 agcgccagcc aaaaaagaag cgtatcgctg gaaggcgacc actccggtga tgcagcaaaa
    14581 agaagtggtc gccacgccga aggcgctgaa aaaagcgctg gaacatgaaa aaacgccgga
    14641 actggcggcg aagctagcgg cagaagccat tgagcgcgac ccgtgggcgg cacgggtgag
    14701 tcaactttcg ctaccaaaac tggtcgaaca ggtggcctta aatgcctgga aagaggagag
    14761 cgacaacgca gtatgtctgc atttgcgctc ctctcagcgg catttgaaca accgcggtgc
    14821 acagcaaaaa ctggctgaag cgttgagcac gttaaaaggt tcaacggttg aactgactat
    14881 cgttgaagat gataatcccg cggtgcgtac gccgctggaa tggcgtcagg cgatatacga
    14941 agaaaaactt gcgcaggcgc gcgagtccat tattgcggat aataatattc agaccctgcg
    15001 tcggttcttc gatgcggagc tggatgaaga aagtatccgc cccatttgat cgtaagcaca
    15061 gcttacgttc gtcatcctta acgtgattga gagagaaacc tatgtttggt aaaggcggtc
    15121 tgggtaacct gatgaagcaa gcccagcaga tgcaagaaaa aatgcagaaa atgcaggaag
    15181 agatcgcgca gctggaagtc accggcgaat ctggcgcagg tctggtaaaa gtgaccatca
    15241 acggtgcaca caactgccgt cgcgtagaga tcgacccgag cctgctggaa gacgacaaag
    15301 agatgctgga agacctggtg gctgcagcat tcaacgacgc agcacgtcgt attgaagaaa
    15361 cgcagaaaga aaaaatggcc tctgtatcct ccggaatgca gctgccgcct ggctttaaga
    15421 tgccgttctg atgcaaacca gcccgctgtt aacacagctt atggaagcac tgcgctgtct
    15481 gtcgggcgtt ggcccgaagt cggcgcagcg tatggcgttc acgctgcttc agcgcgatcg
    15541 tagcggcggg atgcgtctgg cgcaggcgct cacccgggcg atgtcggaaa tcggccactg
    15601 cgccgattgc cgcactttca ccgaacagga agtctgtaac atctgttcga atccgcgtcg
    15661 tcaggaaaac ggtcaaatct gtgtggtgga gagtccggcg gacatctacg ccattgagca
    15721 gacggggcag ttttctggtc gttattttgt gttgatgggg catctgtcac cgctggacgg
    15781 catcggtccg gatgatatcg ggcttgatcg tctggaacag cgtctggcag aggaaaaaat
    15841 cactgaagtg atcctcgcca ccaatcccac ggttgaaggt gaagctaccg ctaactacat
    15901 tgccgagctt tgcgcgcaat atgacgtgga agctagccga atcgctcatg gcgtaccggt
    15961 tggcggcgaa ctggaaatgg tcgatggcac cacgctgtca cactcccttg ccgggcgtca
    16021 taagattcgt ttttaagcaa acgagagcag gatcacctgc tctcgcttga aattattctc
    16081 ccttgtcccc atctctccct catcctgttt ttaaccttaa aatggcatta ttgaggtaga
    16141 cctacatgaa aggacaagaa actcgtggtt ttcagtcaga agtgaaacag cttctgcacc
    16201 tgatgatcca ttctctctat tccaataaag aaatcttcct gcgtgagctt atctctaacg
    16261 cctccgatgc ggcggacaag ctgcgtttcc gtgcgctatc taacccggac ctgtacgaag
    16321 gtgatggcga actgcgcgtt cgtgtctctt tcgataaaga caagcgcacg ctgaccatct
    16381 ccgataacgg cgtggggatg acccgcgacg aagtgattga ccatctgggg actatcgcta
    16441 aatccggtac caaatcattc ctcgaatccc tgggttctga ccaggcgaaa gacagccagc
    16501 tgatcggtca gtttggtgtt ggtttctact ctgcgtttat cgtggccgac aaagtgactg
    16561 tgcgtactcg cgcggcaggc gaaaaaccag aaaacggcgt cttctgggaa tcggctggcg
    16621 aaggtgaata taccgttgcc gacatcacca aagaagatcg tggtactgaa atcaccctgc
    16681 atctgcgtga aggcgaagac gagttcctcg atgactggcg cgtgcgttcc atcatcagca
    16741 aatactccga ccatatcgcg ctgccggtag agatcgaaaa acgcgaagag aaagacggcg
    16801 aaaccgttat ctcctgggag aaaatcaaca aagcgcaggc gctgtggact cgtaacaagt
    16861 cggaaatcac cgatgaagag tacaaagagt tctacaaaca catcgcccac gactttaatg
    16921 atccgctgac ctggagccac aaccgcgttg aaggtaagca ggagtacacc agcctgctgt
    16981 acatcccgtc ccaggctccg tgggatatgt ggaaccgtga tcataaacac ggcctgaaac
    17041 tgtacgtcca gcgtgtgttc atcatggacg acgcagaaca gttcatgccg aactatctgc
    17101 gcttcgtgcg tggtctgatt gactccagcg atctgccgct gaacgtttcc cgtgaaatcc
    17161 tccaggacag cacggtaacg cgtaacctgc gcaatgcgct gaccaagcgt gtgctgcaaa
    17221 tgctggaaaa actggcgaaa gacgacgcgg aaaaatacca gaccttctgg caacagtttg
    17281 gcctggtact gaaagaaggt ccggcggaag atttcgctaa ccaggaagcg atcgccaaac
    17341 tgctgcgttt tgcttctacc cataccgatt cttctgcgca gaccgtatct ctggaagact
    17401 acgtttcccg catgaaagaa gggcaggaga aaatctacta catcaccgca gacagctatg
    17461 cggcagcgaa gagcagcccg cacctggaac tgctgcgtaa gaaaggcatc gaagttctgc
    17521 tgctttccga ccgcatcgat gagtggatga tgaactatct gactgagttc gacggtaaac
    17581 cgttccagtc ggtgtctaaa gttgacgagt cgctggaaaa actggctgac gaagttgatg
    17641 agagcgcgaa agaagcggag aaagcactga ctccgttcat cgaccgtgtg aaagccttgc
    17701 tcggcgagcg cgtgaaagat gtccgtctga ctcaccgtct gaccgatacg ccagcgatcg
    17761 tttcgaccga cgcggacgaa atgagcactc agatggcgaa actgttcgct gcggcgggcc
    17821 agaaagtgcc agaagtgaaa tacatcttcg aactgaaccc ggatcacgta ctggtgaaac
    17881 gtgcggcaga tactgaagat gaagcgaagt tcagcgagtg ggtagaactg ctgctggatc
    17941 aggcgctgct ggcagaacgc ggcacgctgg aagatccgaa cctgtttatt cgtcgtatga
    18001 accagctgct ggtttcctga tgtcatgccg gatgaccttt gtgtcatccg gcatttttct
    18061 tttcatcatc tgcactttcc gcaaattatc tcgccattaa ccgtttcagc ctcaggtgcc
    18121 tttcttgagg caatcgcctg ttggtggtat cgtttatcgc tttttcaaaa aattcgacac
    18181 attttaaggg gattttcgca atgcgtatca ttctgcttgg cgctccgggc gcggggaaag
    18241 ggactcaggc tcagttcatc atggagaaat atggtattcc gcaaatctcc actggcgata
    18301 tgctgcgtgc tgcggtcaaa tctggctccg agctgggtaa acaagcaaaa gacattatgg
    18361 atgctggcaa actggtcacc gacgaactgg tgatcgcgct ggttaaagag cgcattgctc
    18421 aggaagactg ccgtaatggt ttcctgttgg acggcttccc gcgtaccatt ccgcaggcag
    18481 acgcgatgaa agaagcgggc atcaatgttg attacgttct ggaattcgac gtaccggacg
    18541 aactgattgt tgatcgtatc gtaggccgcc gcgttcatgc gccgtctggt cgtgtttatc
    18601 acgttaaatt caatccgccg aaagtagaag gcaaagacga cgttaccggt gaagaactga
    18661 ctacccgtaa agacgatcag gaagaaaccg tacgtaaacg tctggttgaa taccatcaga
    18721 tgactgcacc gctgatcggc tactactcca aagaagcgga agcgggtaac accaaatacg
    18781 cgaaagttga cggcaccaag ccggttgctg aagttcgcgc tgatctggaa aaaatcctcg
    18841 gctaatcaag ccgcagcaga tctgctttcg ggcaggtctg ctcatccacc tcaggcaata
    18901 cctctcacag caattagttc ttcttcctca cttttccgct acaattatca acaagttgaa
    18961 tcgataagag gcggtaatgc gtcagactaa aaccggtatc ctgctggcaa acctgggtac
    19021 gcccgatgcc cccacacctg aagcggtaaa acgctatctg aaacaatttt taagcgacag
    19081 acgcgtggtt gatacctcac ggttgttatg gtggccattg ctgcgcggcg ttattttgcc
    19141 gctgcgctcg ccgcgtgtgg cgaagctgta tgcctctgtc tggatggaag gtggctcgcc
    19201 gctgatggtt tacagccgcc agcaacagca ggcgctggca caacgtttac cggagacgcc
    19261 cgtagcgctg ggaatgagct acggctcgcc atcactggaa agcgccgtcg atgaactcct
    19321 ggcagagcat gtagatcata ttgtggtgct gccgctttat ccgcaattct cctgttctac
    19381 ggtcggtgcg gtatgggatg aactggcacg cattctggcg cgcaaacgta gcattccggg
    19441 gatatcgttt attcgtgatt acgccgataa ccacgattac attaatgcac tggcgaacag
    19501 cgtacgcgct tcttttgcca aacatggcga accggatctg ctgctgctct cttatcatgg
    19561 cattccccag cgttatgcag atgaaggcga tgattacccg caacgttgcc gcacaacgac
    19621 tcgcgaactg gcttccgcac tggggatggc accggaaaaa gtgatgatga cctttcagtc
    19681 gcgctttggc cgggaaccct ggctgatgcc ttataccgac gaaacgctga aaatgctcgg
    19741 agaaaaaggc gtaggtcata tacaggtgat gtgcccgggc tttgctgcgg attgtctgga
    19801 gacgctggaa gagattgccg agcaaaaccg tgaggtcttc ctcggtgccg gcgggaaaaa
    19861 atatgaatat attccagcgc ttaatgccac gccagaatat atcgaaatga tggctaatct
    19921 tgttgccgcg tatcgctaaa gctgagcggt aaagaactga gcgccgtcgc gaagagcctc
    19981 gtcggcggtt ttcatcattc gtgaataatg caaaaaggca tgcagcgtac ctgggtagag
    20041 tttgaactca cagggctgct gatgcgccgc taacgtctgg taaagcagac ggctgtcatc
    20101 cagcagcgga tcgaactccg ccccggcaat aaaacagggc ggaacttcgc gagtgagatc
    20161 attattaaac agacagtaat acggtgactc gcggtccgcg tcgttgctta aatacgcctc
    20221 ttcatacatc tgcaaatcct gttgcgttaa gccatcccag acaccgccca acagacgacg
    20281 agtcacggaa tcccgtaatc cgtaaagccc ataccacagc aaaacacccg caattttacc
    20341 gcaatcgatc tgtttatcac gcaaccacaa cgcactggcg agcgccagca tggcacctgc
    20401 ggaatcaccg gcaaagccaa tgcgggacat attgatttga taatcctccg cctgctggtg
    20461 gaaataacaa caagcagcca caatttcctc tatcgcttgc ggaaaacgcg cttcaggtga
    20521 aagggggtaa tcaataccaa tcaccgtaca ttggctgtag cttgccagca ggcgcatgat
    20581 gcgatcgtgg gtatcgagat tgccgagaat aaaaccgcct ccatgcaaat aaaatagcgt
    20641 cgccgggcta tctggctgcg gacaaaagag acgtgtttcc acctgcccat attttgttgg
    20701 aaccatgtaa gctctggttg ccatttctgg agcgcccgca ttccagaatc ggcgctcaag
    20761 cgtgtaatac tgtcgttgct cagcaatcgt tcccgttgcg ggccaggacg gtaaatccgg
    20821 ctgaagagta ttcacaacgg tcttcatttc agcagaaata aggtccagaa caggtagttt
    20881 gttttccggc ttcatacaaa aactcctttc aaattacgtc attgtaagga accactgcca
    20941 tgaaaatgcg atcccgcctg ctgatattga aactggctgc gtctcgcgcg ctcccgtcag
    21001 attgtgttaa cattcgccgc tcagttaacc acccgtaaaa acaaccatga aatttcccgg
    21061 taaacgtaaa tccaaacatt acttccctgt aaatgcacgc gatccgctgc ttcagcaatt
    21121 ccagccagaa aacgaaacca gcgccgcctg ggtcgtaggt attgaccaga cgctggtcga
    21181 tattgaagcg aaagtggatg atgaattcat tgagcgttat ggattaagcg ccgggcattc
    21241 actggtgatt gaggatgacg tagccgaagc gctttatcag gagctaaaac agaaaaacct
    21301 gattacccat cagtttgcgg gtggcaccat tggtaacacc atgcacaact actcggtgct
    21361 cgcggacgac cgttcggtgc tgctgggcgt catgtgcagc aatattgaaa ttggcagtta
    21421 tgcctatcgt tacctgtgta acacttccag ccgtaccgat cttaactatc tacaaggcgt
    21481 ggatggcccg attggtcgtt gctttacgct gattggcgag tccggggaac gtacctttgc
    21541 tatcagtcca ggccacatga accagctgcg ggctgaaagc attccggaag atgtgattgc
    21601 cggagcctcg gcactggttc tcacctctta tctggtgcgt tgcaaaccgg gtgaacccat
    21661 gccggaagca accatgaaag ccattgagta cgcgaagaaa tataacgtac cggtggtgct
    21721 gacgctgggc accaagtttg tcattgccga gaatccgcag tggtggcagc aattcctcaa
    21781 agatcacgtc tctatccttg cgatgaacga agatgaagcc gaagcgttga ccggagaaag
    21841 cgatccgttg ttggcatctg acaaggcgct agactgggta gatctggtgc tgtgcaccgc
    21901 cgggccaatc ggcttgtata tggcgggctt taccgaagac gaagcgaaac gtaaaaccca
    21961 gcatccgctg ctgccgggcg ctatagcgga attcaaccag tatgagttta gccgcgccat
    22021 gcgccacaag gattgccaga atccgctgcg tgtatattcg cacattgcgc cgtacatggg
    22081 cgggccggaa aaaatcatga acactaatgg agcgggggat ggcgcattgg cagcgttgct
    22141 gcatgacatt accgccaaca gctaccatcg tagcaacgta ccaaactcca gcaaacataa
    22201 attcacctgg ttaacttatt catcgttagc gcaggtgtgt aaatatgcta accgtgtgag
    22261 ctatcaggta ctgaaccagc attcacctcg tttaacgcgc ggcttgccgg agcgtgaaga
    22321 cagcctggaa gagtcttact gggatcgtta agttatcgtc ggttcgtagg ccagataagg
    22381 cgttcacgcc gcatctggca tttggctctc gatgcctgat gcgacgctgg cgcgtcttat
    22441 catgcctaca tatttttcat attttacatc cggcaaccac cgtttacccc gtcaccacct
    22501 cacccgccgg tggcgtttcc agcagttcca gcatggtacg ggcgatttca cgctcgccca
    22561 tcactacctg attcgcacca cgttcggtga tatacgccac ttcatcgtca taatgggcgc
    22621 gggcaataat ctcaatatcc ggatttttcg cgcgggcaga tgccacaatc tcacccgctt
    22681 cataaccgtt gggaatcgtc aggatcagcc attttgcaca ttccagatgc gccagttgca
    22741 taatttcttc gttcgccgca ttgcccaata ctgcgcggac cccgcgctct cgcagctcat
    22801 caacacgggt tcgtgacgtc tcaatcacca ccagcggaat atcagaggcg agcaatttct
    22861 cccccagcag gctgcctaca cgaccgtaac ccaccagtag cgcatggttg caaatatcca
    22921 ctgggatctg cttctcttct tcgattgcct cttccagcgt ctgctcttcc agcgtttcgg
    22981 tcttcgccag atatttctcc agtagtgcga acagtaccgg gttgagcata atcgacagga
    23041 tcgcccctgc cagtaccagg ttttgtccgg cctgcggcag taaattcaat gccattccca
    23101 gtcccgccag gataaacgcg aactcaccaa tctgcgccag gctggcggcg atggttaatg
    23161 ccgtacgttg ggagtgacca aacagtcgca ccaggaaaaa tgcggctaac gacttaccaa
    23221 acagaataat cgccagcgtc gccagcactg ccagcggttg ctgaatcaga attaacggat
    23281 caaacaacat cccgacggag acaaaaaaca gcaccgcaaa cgcgtcgcgc aatggcagcg
    23341 tatcgtgggc ggcacggtga ctcagttcag actcgttcag taccatcccg gcaaagaacg
    23401 caccgagtgc aaaggagaca tcaaacagct ctaccgcacc aaaggcaatc cctaacgcca
    23461 gcgccagcac cgacagggta aacagctcgc gagaaccggt tgccgcgctg cgtgccataa
    23521 tccacggcac cagacggcga cctaccagca tcataatggc gataaatgcg atcactttgc
    23581 cgatggtgat ccccatatcg actgcaagag tggcaaagcc cacatcgccc tgttccatca
    23641 ttcctgcaac tgcgggcagc aacaccagcg tcagaaccat taccaggtct tccacaatca
    23701 accaaccgat ggcgatttgc ccacgctgac tgtcaattaa ttgccgttct tcaagtgcgc
    23761 gcagtaacac cacggtactg gcggtggaaa gacataaacc gaacacgata ccggtcatta
    23821 acgaccagcc cagcacggca gagagcgcca tacccagcag cgtcgccacg gctatctggg
    23881 cgatcgcacc gggaatggcg atggccttta ccgccatcaa atccttcagc gaaaagtgca
    23941 aaccgacgcc aaacatcaac agaatgacgc ccagttcagc cagttccggc gcaagcttgg
    24001 tatcggcaac aaagcccgga gtgaatggtc ctgccagcac acccgctaac agatatccca
    24061 ccagaggaga aatacgtagt ttattggcca gcatgccgag gataaaggcg agcacaaggc
    24121 cgccaacaat ggtggtgata agcggggtgg cgtgatgcat tccgtctcct tttcctggtg
    24181 gttattgtcc atttttggcc gggaaaacca aaattacagg taatagttta tgacaatttc
    24241 attgatgatg ttcatgaata attattgaat tttgcagaaa aatggaatta gctgcaaaaa
    24301 aagcacggat cggaaaacag aaggcggttt aacagaggaa aggcttatgg cgactgtaaa
    24361 acgatgcagc caaagtttgc ctttggctgc aatgaaatca gtctttatgc cggttatcag
    24421 gcaggaatat ggtcaacatc cccaatagtg gcaggaaagc acagatttta tagactaact
    24481 cgatgctggt gtgatcggcg ataagcccca gaactgccgc tcccagacct cccatgccaa
    24541 aagcaaaacc gaaaaagagt ccagaaacca taccgatacg tcccggaagt agctcctgag
    24601 cgtagaccag aatggcagag aatgccgaag cgaggataaa tccaataatc accgttaaaa
    24661 cccccgtcca gtgcaggctg gcgtagggta aaatcagcgt aaacggcgca acgccgagga
    24721 tagagcccca aatcacatat ttccgtccaa ttttatcccc tacaggcccg ccgatcaccg
    24781 tacctgccgc aacggcaaac aggaaggcaa acagatgaag ctgggcattc tggatagata
    24841 atccgaattt ttgcatcaga taaaaggtgt aatag
//
LOCUS       DAJTMC010000046        24823 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.046, whole genome shotgun sequence.
ACCESSION   DAJTMC010000046 DAJTMC010000000
VERSION     DAJTMC010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24823
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.046"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(82..2301)
                     /locus_tag="OO130_001671"
     CDS             complement(82..2301)
                     /locus_tag="OO130_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HCR5365126.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSSEEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVLHPAYGNVRIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKKPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(2412..3824)
                     /gene="ftsP"
                     /locus_tag="OO130_001672"
     CDS             complement(2412..3824)
                     /gene="ftsP"
                     /locus_tag="OO130_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HCR5365127.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDSRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(3899..4636)
                     /gene="plsC"
                     /locus_tag="OO130_001673"
                     /gene_synonym="parF"
     CDS             complement(3899..4636)
                     /gene="plsC"
                     /locus_tag="OO130_001673"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HCR5365128.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(4869..7127)
                     /gene="parC"
                     /locus_tag="OO130_001674"
     CDS             complement(4869..7127)
                     /gene="parC"
                     /locus_tag="OO130_001674"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HCR5365129.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LVSERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSLMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(7673..8155)
                     /locus_tag="OO130_001675"
     CDS             complement(7673..8155)
                     /locus_tag="OO130_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HCR5365130.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPKAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTVWGIIRHWLPASGEKMRKAPILFHYTNLAEGMTEQRLETDVYVPLA
                     "
     gene            complement(8208..8600)
                     /gene="ygiW"
                     /locus_tag="OO130_001676"
     CDS             complement(8208..8600)
                     /gene="ygiW"
                     /locus_tag="OO130_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HCR5365131.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            8752..9411
                     /gene="qseB"
                     /locus_tag="OO130_001677"
     CDS             8752..9411
                     /gene="qseB"
                     /locus_tag="OO130_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
                     /protein_id="HCR5365132.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLASEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            9408..10757
                     /gene="qseC"
                     /locus_tag="OO130_001678"
     CDS             9408..10757
                     /gene="qseC"
                     /locus_tag="OO130_001678"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HCR5365133.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNVQGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVVDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPSGQTATGSGLGLSIVQRIAKLHGMNVEFGNAEQGGFEAKVSW"
     gene            10867..11448
                     /gene="mdaB"
                     /locus_tag="OO130_001679"
     CDS             10867..11448
                     /gene="mdaB"
                     /locus_tag="OO130_001679"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HCR5365134.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            11479..11793
                     /locus_tag="OO130_001680"
     CDS             11479..11793
                     /locus_tag="OO130_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCR5365135.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(11841..13733)
                     /gene="parE"
                     /locus_tag="OO130_001681"
     CDS             complement(11841..13733)
                     /gene="parE"
                     /locus_tag="OO130_001681"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HCR5365136.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQIYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(13762..14343)
                     /gene="yqiA"
                     /locus_tag="OO130_001682"
     CDS             complement(13762..14343)
                     /gene="yqiA"
                     /locus_tag="OO130_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HCR5365137.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(14343..15170)
                     /gene="cpdA"
                     /locus_tag="OO130_001683"
     CDS             complement(14343..15170)
                     /gene="cpdA"
                     /locus_tag="OO130_001683"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HCR5365138.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(15195..15617)
                     /locus_tag="OO130_001684"
     CDS             complement(15195..15617)
                     /locus_tag="OO130_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HCR5365139.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(15618..16247)
                     /gene="nudF"
                     /locus_tag="OO130_001685"
     CDS             complement(15618..16247)
                     /gene="nudF"
                     /locus_tag="OO130_001685"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HCR5365140.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHSLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            16452..17933
                     /gene="tolC"
                     /locus_tag="OO130_001686"
     CDS             16452..17933
                     /gene="tolC"
                     /locus_tag="OO130_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HCR5365141.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSIVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            18081..18752
                     /locus_tag="OO130_001687"
     CDS             18081..18752
                     /locus_tag="OO130_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HCR5365142.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLASCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            18758..19918
                     /locus_tag="OO130_001688"
     CDS             18758..19918
                     /locus_tag="OO130_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCR5365143.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLPLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(19956..20744)
                     /gene="ygiD"
                     /locus_tag="OO130_001689"
     CDS             complement(19956..20744)
                     /gene="ygiD"
                     /locus_tag="OO130_001689"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HCR5365144.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPVLAQHLVELLAPVPVT
                     LDKEARGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWAMSFNEYVKANLTWQGAVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            20887..21648
                     /gene="zupT"
                     /locus_tag="OO130_001690"
     CDS             20887..21648
                     /gene="zupT"
                     /locus_tag="OO130_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HCR5365145.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGISPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAVAGIMVALSVDELMP
                     LAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(21706..21876)
                     /locus_tag="OO130_001691"
     CDS             complement(21706..21876)
                     /locus_tag="OO130_001691"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5365146.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(22136..22789)
                     /gene="ribB"
                     /locus_tag="OO130_001692"
     CDS             complement(22136..22789)
                     /gene="ribB"
                     /locus_tag="OO130_001692"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HCR5365147.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(22893..23041)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            23163..23453
                     /gene="ubiK"
                     /locus_tag="OO130_001693"
     CDS             23163..23453
                     /gene="ubiK"
                     /locus_tag="OO130_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HCR5365148.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRMSELENRSTEIKKQPDPETLPPTL"
     gene            23737..24288
                     /locus_tag="OO130_001694"
     CDS             23737..24288
                     /locus_tag="OO130_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HCR5365149.1"
                     /translation="MSAFKKSLLVAGVAMILSNNVFADEGHGIVKFKGEVISAPCSIK
                     PGDEDLTVNLGEVADTVLKSDQKSLAEPFTIHLQDCMLSQGGTTYSKAKVTFTTANTM
                     TGQTDLLKNTKETEIGGATGVGVRILNSQSGEVTLGTPVVITFNNTNSYQELNFKVRM
                     ESPGKDATPGNVYAQADYKIAYE"
     gene            24348..>24823
                     /locus_tag="OO130_001695"
     CDS             24348..>24823
                     /locus_tag="OO130_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR5365150.1"
                     /translation="MYKKLKLTTISELIKNIYCSLSVLIIGCASAYAVEFNKDLIEAE
                     DRENVNLSQFETDGQLPVGKYSLNAIINNKRTPIHLDLQWVLIDNQTAVCLTPEQLTL
                     LGFTDEIIEEAQQNLIDGCYPIEKEKQITTYLDKGKMQLSISAPQAWLKYKDANWTP"
BASE COUNT         6192 a         6581 c         6169 g         5881 t
ORIGIN      
        1 tcggcatata aataaatgcc taatgagata gattctgatg taatatcccc ggtagcaatt
       61 ctgctaccgg gaacagcgaa tttaacgtcc aaccgccgct ttaggccgtt tcttcgcacc
      121 agcattcacc ggacgagatt gcgtagacga cgcttttttt gccgtagcag gcgtctgacg
      181 ctgggtcgcc atcggcgtat gtttcgtcaa cgccggacgg gtattgcggt tctggcgacg
      241 agcttcacgc atctcttcaa tggttggcgc aggcactaag caatcgcgac ggctaccaat
      301 cagatgcttt ttacccatcg cttccagcgc ctggcggatt aacggccagt ttgccggatc
      361 gtggtaacgc aacaacgctt tatgcaaacg acgctgcttg tcgcccttcg gtacgaagac
      421 gtcttcactc ttataaccaa tcttcgccag cggttttttc ccggtgtaat acatggtggt
      481 tgagttcgcc agcggcgatg ggtagaagtt ctgtacctgg tcgagacgga aacgatgctt
      541 tttcagccac agcgccagat tcaccatatc ttcatcacgc gtaccggggt gcgcggagat
      601 gaaatagggg atcagatact gctctttacc tgcctgtttt gagtaagtat cgaacagctc
      661 tttaaagcgg tcatagctgc ccatgcccgg cttcatcatc ttcgataacg gcccttcttc
      721 ggtatgttcc ggggcaatct tcagataacc gccgacgtga tgggtcgcca gttctttgat
      781 atagcgcgga tcttccacgg cgatgtcata acgcacacca gaggcaatca ggatcttttt
      841 aatgcctttc agatcacgcg cacggcgata gaggttgatc gttggttcgt ggttcgtgtc
      901 catgtgcgga caaatatccg gataaacgca cgacaaacgg cggcaggttt gctcagcgcg
      961 tggcgatttg cagcgcaaca tatacatgtt ggcagttggc ccaccgagat cggaaattac
     1021 gcccgtaaaa cctggaacgg tgtcgcggat cgcttcgatc tcattaatga tcgaatcttc
     1081 ggaacggctc tgaataatgc gcccttcgtg ctcggtgata gaacagaaag agcagccgcc
     1141 aaagcagcca cgcataatgt tgaccgaaaa acggatcatt tcgtaagccg gaatacggac
     1201 attgccatag gccggatgta gcacgcgctt gtatggcagc gcaaaaacgc tgtccatctc
     1261 ttcggtagaa agcggaatag caggcgggtt gatccacaca tagcggtcgc cgtgtttttg
     1321 catcaatgcg cgggcacagc ctgggttggt ttcgtggtgc agaatacgcg aagcatgggc
     1381 gtacagcact ttatcgccct tcactttctc gaaagaaggc agcaacacgt aggttttttc
     1441 ccacggtttc gggcgcggtg gctgcacggt tacggctttg gcttcctgct ttttcggtgc
     1501 caccggtttg ttatccgcgc acggcaaatc ttcaccatac ggatgcggga ttgggtcgat
     1561 ttttccaggg gtatcaagac gggtggaatc cacgccgctc cagccaggca gggcctcttt
     1621 cacgataatc gcggtattac gcacatcgcg gatttcacta atcggctcgc ccatcgccag
     1681 acggtgcgcc acctccacca gcggacgctc accgttacca aacatcagca tgtcggcttt
     1741 cgaatccacc agcacggaac ggcgcacggt atcggaccag taatcataat gtgcggtacg
     1801 acgcagacta gcctcaatac cgccgaggat caccggtaca tctttccacg cctctttaca
     1861 acgctgggta taaaccagtg tggcgcgatc cgggcgctta cccgcgacgt tatccggcgt
     1921 gtaggcatcg tcatgacgta aacggcgatc ggcggtataa cggttgatca tcgaatccat
     1981 gttgccagca gtaacaccga aaaacagatt cggtttaccc agacgcataa agtcgtcttt
     2041 gctgctccag tccggctggg cgatgatccc gacgcgaaag ccctgcgctt ccagcatacg
     2101 accgcaaatc gccatcccga agcttgggtg atcgacatac gcgtcgccag taaccaaaat
     2161 gatgtcgcag ctatcccagc caagttgatc catctcttca ctagacatcg gcaaaaacgg
     2221 tgccggtcca aaacaggcgg cccagtactg cggccaggag aacaggtcgc gatccggttg
     2281 gatcagggag atagagctca ttttgcttcc agaaatgata aaaaaataat caaaggccgg
     2341 ggattataag ccggaacgaa agagaaatcg aaaagtattc catactcgcc ctcctcgggc
     2401 gagtatgaag attacggtac cggattgact aacagttgcc caatcgagcc acggtccgcc
     2461 atttccagcg tctgactgtt gaagtagaac gggaagtgcg cccaggaagg ctgaccgaaa
     2521 taaacaagca gctccacttg tccgtcaacc caaacggtat ctttccagcc tctgtcttcc
     2581 gggaacggca tcgcaccatt cacgttacgg atctggaaca tcacgccttc aatatggaac
     2641 gcttgcggct cgtccgcgcg taccgtccag cgttcccacg ttccttgctg cgcggtgaca
     2701 tcaatacggt tgacgtccca cagttgtcca ttaatacccg ggtcatcacc cagactgata
     2761 tcgcgactgc gaatcggcga accggccatg atttcagttg gcagcaagcg catcggaaga
     2821 ctgtctgtga ccagcggcag aaggccggtt gggcgcagcg ttagcaccag ggtagaaacc
     2881 agaatgctgg atggctcaaa gaagccacga atacgatcaa caatgctcgc cgcttcgcca
     2941 caggtgatcg acacttcatc accgttgctc atatccacca gaatctcgcg gcgctcgccc
     3001 ggtgccaacg aaagttgctt caccgataca ggagcaggca ggaatccctg atcgccagaa
     3061 atcacatgta acgggcgact atcgctcatc tgtagttgat agcgacgaga gttcgacgcg
     3121 ttcagcagtc gcaagcgcac ccagccacgc gagacttcaa cgtacgggct ttgtacaccg
     3181 ttaaccagca gcgtatcacc aacaaagccg ccgcttcccg gttcgttgta ttctggcgta
     3241 ccaaagttat ccagccgttt atcctggata atgaccggaa aatcatccac accataatgg
     3301 ttggggatag gcagcgactt gctgacttca tcttccacca gccacattcc ggcaaggccg
     3361 ttatagacct gctgagccgt gcggttggga gtattggcgt gataccacag agtagctgcg
     3421 ttctggcgaa tgggcagtac gggtgcccag tcagcgtttg gcgacatcat ccgtgccgga
     3481 ccgcccatca gcgggcctgg tacctgtagc ccggccaccg tcattgagac attttctgtc
     3541 aggcggttgc tgtaaataag cttaacatcg tcgcccttcc agacgcggat agtcggcccc
     3601 aggtaacgac cattgattcc ccagaccgac gcgcgtgtcc ctggcgtaaa tgaccagtgc
     3661 gcacgttgta cagtcataaa cagcggttgc ccacggcgag attcaagtag cggcggaacg
     3721 ggtagcggtt gctgttgccc ggctgcgctg gccttcaggg gaacagcgcc ggcacaaagt
     3781 gcaatccccg atgcctgaat gaactgacgc cgactgagtg acatatttgc tccatgtaaa
     3841 actggctaaa gtaataccgt gcaggaaagt gttccccgca aaaatacaga ttgccgactt
     3901 aaacttttcc ggcggcttcg cgttctgcga cttctttatc gagctcggcg attttttgtt
     3961 ccattatcga acgacaatgg gcagccagct cacgaacctg atctttgcca tactgactga
     4021 cgtcaattgg cggcagcatt tcgacaatca ccagaccgtt gtgcagtcga ttaagattaa
     4081 tcttattcga agttgtagag acgcacacgg gaataatcgg gacgcccgcc gcaattgccg
     4141 cgtgaaatgc tccagtcttg aacggtagca ggccgcgacc acggctgcgg gttccttccg
     4201 ggaacatcca gatggaaata cggcgttttt tgaaatgatt cacgacttcc gcaatggtgc
     4261 cgtgagcttt agtgcgattg tttctgtcga tcaataagtt gccggttaac cagtacaact
     4321 gcccgaagaa ggggatccac agcaagctct ttttacctac cgtcaccgtc ggcggttgca
     4381 caatgttcga tgctgtcacc atgtcatagt tgttctggtg gttagcgata tagatagcat
     4441 tgccgtagct ttcagcatct gcaggtttgc ggcactcaac tttcaggccg aacagcggcg
     4501 caagacggcc aaacatatgc ccaaaggtgg ccacatgttt cgggttacgc gggctgaaaa
     4561 ggcagtaaat ggagccgaat acacagacta agatgctgta aatcacggta ataataagac
     4621 gaaagatata tagcataaca acctctgaag gtctgagaga ctggcattgt acgtcacctg
     4681 aaatcaggtt agggcattgt taaaagccgc tctctggaaa agcgtattgt taatcgcaac
     4741 gcatgaataa acaacggttt caagggaatt tttatcgttt ccgggggttg aatacgctgc
     4801 cggatgacga cttaacgttt catccggcgt tccttgcaag cgggaggaaa caggccctcc
     4861 ccggcatatt actcttcgct atcaccgctg ctggcacggc gaggagagtc gatctcaaca
     4921 cgatcgatac gctgcaaacc gcgcatcaac gtaccgcggc gtccacgttc gccagtgact
     4981 ttctgtaact cttccgggcg cagtttaatt ttgcgtttcc caacatgaat ggtcagcgtg
     5041 ctttgcggcg gcagaacgta caattgcgcc agaccatctt ctccacgcgc ggcttctgcc
     5101 gatggaatgt tgataatctt gttgcctttg cccttcgaca gctgcggcag atcgcttacc
     5161 gggaacatca acatacggcc tgcctgagtg attgccagca gcatatcgga agcatcttca
     5221 atcaccaccg gcggcataac atgggcattt tccggtaagg tgatcaaagc cttacctgca
     5281 cggttacgcg ccaccagatc gttaaaggtg cagacgaaac cgtaacccgc atcggaagcc
     5341 atcagcagtt tctgatcgtc gctttccatc agcatatggt caacggtcgc cccaggcggc
     5401 aacgttaatt tgccggtgag tggttcgccc tgaccacgcg ccgacggcag cgtaatcggg
     5461 tcgatggcat agctacgacc ggtggaatca acaaacacta ccggttggtt gctcttacct
     5521 ttcaccgccg ctttgaagct atcacccgct ttataattca ggcccggcgc gtcgatatca
     5581 tggcctttag cgctgcgtac ccagcccatc agcgacagca caatggtgac aggttcagac
     5641 ggcagcatgt cgtgctcgct catcgctttc gcttcttcgc gttcctgcaa cggcgaacga
     5701 cgatcgtcac cgtaggcttg cgcgtctgcc tgcagttctt tcttcagcag gttattcatt
     5761 ttacgctcgg aaaccaaaat gccctgcaac tggtcgcgct ctttttccag ctcactctgc
     5821 tcaccgcgaa tcttcatctc ttccagtttg gcaagatgac gcagtttcag ttcgaggatc
     5881 gcttccgcct gggtttccgt aaggccaaac cgcgacatca gcgccggttt cggttcatct
     5941 tcgttacgaa tgatctcaat cacttcgtcg atgttgagaa acgccaccag caaaccttcc
     6001 aggatatgca ggcgcttgag gactttctcc agacgatagt tcagtcggcg gcgcacggta
     6061 tcgcgacgga acaccagcca ttcggagagg atttccagca ggtttttcac cgccggacga
     6121 ccatccagac cgatcatatt gaggttaatg cgatagctct tttccagatc ggtggtagcg
     6181 aagaggtggt tcatcacctg atccatatcc acgcggttgg aacgcggcac aatcaccaga
     6241 cgggtcgggt tctcgtggtc agattcatcg cgcagatcgt caaccatcgg cagctttttg
     6301 ttgcgcattt gcgcagcaat ttgctccagt acgcgcgcac ctgaaacctg atgcggcaat
     6361 gcgctgataa ccaccgcgcc atcttctttc ttccacaccg cgcgcatacg cactgaacca
     6421 cgtccgttct cgtagatttt acggatctcg gcgcgcgaag tgataatttc cgcttcagtc
     6481 ggataatccg gcccctgcac gatatccagc agctgatcga gcgtggtttt cggctggtcg
     6541 attaatgcga ttgccgcctg agctacttca cgcaggttat gcggtggaat atcggtcgcc
     6601 atgccgacgg caataccggt ggtgccgtta agcaaaatgt ttggcagacg ggcaggcagc
     6661 attttcggct cctgcaaagt gccgtcgaag tttggcaccc agtcagccgt cccctgcccc
     6721 agctcgctca atagcagttc ggaatatttc gacaaccggg attcggtgta acgcattgcc
     6781 gcgaacgatt tcggatcgtc cggcgcgccc cagttcccct gaccatcaac cagcggataa
     6841 cggtaagaga acggctgcgc catcaggacc atcgcttcat aacaggcact atcgccgtgc
     6901 ggatggtatt tacccagtac gtcaccgacg gtacgggccg attttttaaa tttggcgctg
     6961 gcattcaggc ccagttcaga catcgcatac acaatgcggc gctgaacagg tttaagacca
     7021 tcgccaataa acggcaacgc acggtccatg atcacgtaca tggagtagtt taagtaggcg
     7081 ttttccgtaa attcatgtag cgcaaggcgc tctgccatat cgctcattaa ttctgattcc
     7141 tcaacttatt cgccagcctt caaaggggca atattgccgg agatactaca ttatctgccg
     7201 cgtcgagtca caggagatca gcgtgaatag cgccgccgga cgtgctccga cggcgataaa
     7261 ggcatcaatg tgccttgata tacaactctt tgctgtagac ttgatcggca tagagggcag
     7321 cacaaggttg cgcccatgcc acaccactcg gcatgggggt ccgctccggg cgattcgaga
     7381 agttgaggtt atgcgagtcc agggaagcct aaagcatcaa agtagtcgac tgtaagtata
     7441 cggttcagcc cagtttagct gtgtctccac cagtgccccg cgcctcctgc cataagtgcg
     7501 gcttgctcat ggataccccc gagacggctt acggcgcacc ggataataaa tttcgcgtat
     7561 acatggtatt ccttaccgga gaaattataa gcccaacaac aatacgcctg tggcattgtt
     7621 cgctcaaact tatcgcgagt gatttggctc acactccggt aaagattccc catcacgcta
     7681 acggcacata aacatccgtt tccagtcgct gttctgtcat accttcggca agattggtgt
     7741 agtgaaacag aatcggtgct ttacgcattt tctcgccgct tgcaggcagc cagtggcgaa
     7801 tgatgcccca taccgtgtgt gaaatatcgt ccagctcgcc aacgtggcgg gccacggcat
     7861 aacgtccacc ggtaagttca ccattgctca caccataacg attatcggga atcggttcgc
     7921 taacgctgcc gcagatatca aagcgaaacg ctttcggtgc ggtggtggca gggtcgtccc
     7981 aggcgacgcc aaaagtctgg ctttggttaa caggagaaag ccccgtctct ttacgccaca
     8041 tgataaattt cgccacactg tagttgagca actcagggct acagcggtgc ggcaacatcg
     8101 ccacagggat tgatgggaaa tcgataatgt ttacatccag tgtcaggttt gtcattttgc
     8161 ctccctgcga aattattccc gggaacgcgt cccgggagcg gtaacaatta cggatttact
     8221 ttgcggatct gtttgacgtc aatttcaaca gaattccagt ctttatcgac ttccccctga
     8281 atctcaaccg tatctttcgg cgtcaccgtc acaccgttcc agcgtttgtg gtcgatatca
     8341 acattgatag taccgctggc atctttaaac acgtagagat cgtcagagat gcgttcaacg
     8401 atattgccgc gcagggttac ccaggtgtcg tcacgcaggg attttgcgct ttctacagtc
     8461 gttacgctgc cgttcggccc ctggaatcct ccggcctgac tttgcgttgc cgatgggcca
     8521 gaaaaaccgc cctgctctgc tgccatcacc ggtgcactgc acagggccat tactgcgatt
     8581 actgctgcga attttttcat gtttattact ccctttaatg tctgtttccg agcatttaac
     8641 aagatagtcc ttaacaactt cttaagggaa aaaaataaaa tttaatgctg tacagagcgc
     8701 gttacaacac ggtttactgg cagcaaatac ggttatcgca gggatgaaaa aatgcgaatt
     8761 ttactgatag aagatgacat gctgattggc gacggcatca aaactggcct tagtaaaatg
     8821 ggttttagtg tcgactggtt tacacaaggt cgtcagggaa aagaggcgct ttatagcgca
     8881 ccttatgatg cggtgatcct ggatttaacc ttaccaggca tggatggtcg cgatattttg
     8941 cgcgaatggc gagagaaagg tcagcgtgag ccggtactga tcctgaccgc gcgtgatgcg
     9001 ttagcggaac gtgtagaagg gctgcgtctg ggagctgacg attatctgtg taaacctttt
     9061 gcgttgatag aagtcgccgc caggctggag gctctgatgc gccgaaccaa cggccaggcc
     9121 agcaacgagc tgcgccacgg taacgtcatg ctcgaccccg gcaaacgtat cgccacgctg
     9181 gctagcgaac ccttaacact gaaaccaaaa gaatttgccc tgctggaatt actgatgcgt
     9241 aacgctggtc gggtactgcc gcgcaaactg attgaagaga aactgtatac ctgggacgaa
     9301 gaggtcacca gtaatgccgt tgaagtgcat gtgcatcatc tgcgacgcaa actcggtagt
     9361 gattttattc gtaccgtgca tggtattggc tacacattag gtgagaaatg aaatttaccc
     9421 aacgtcttag tctgcgcgtc aggctgacgc taatcttttt aattctggcc tcggtgacct
     9481 ggctgctttc cagctttgtc gcctggaaac aaacaacgga taacgtcgat gaattgttcg
     9541 acacccaact gatgctgttt gccaagcggt taagtacgct cgatctcaac gaaatcaacg
     9601 cggcggatcg catggcacag acgccaaata aattaaaaca cggtcatgtt gatgacgatg
     9661 cgctgacctt tgccatcttt acccacgacg gcagaatggt ccttaatgat ggcgataacg
     9721 gagaagatat tccctatagc tatcaacggg aaggttttgc tgacgggcaa ctggtcggtg
     9781 aagacgatcc ttggcgtttt gtctggatga cctcacctga tggcaaatat cgcatcgttg
     9841 ttggccagga atgggaatac cgtgaagaca tggcgctggc gattgttgcc gggcaattga
     9901 tcccgtggct ggtcgcactg ccgattatgt taatcatcat gatggtacta ctgggtcgtg
     9961 aactcgcgcc gctgaacaaa ctggcgctgg cactacgtat gcgtgaccct gactcggaaa
    10021 aaccactaaa cgcgactggc gtacccagcg aagtgcgacc actggttgag tcgctaaatc
    10081 aactgttcgc ccgcacacat gcgatgatgg ttcgagaacg acgctttacc tccgatgcag
    10141 ctcacgaact acgtagcccg ttaacagcgc tgaaagtgca aaccgaagtt gcgcagctct
    10201 ctgacgatga tccgcaggca cggaaaaaag cactgctcca attacattcc gggatcgatc
    10261 gcgctactcg tctggttgat caactgctca cgctatcgcg gctggactca ctggataacc
    10321 ttcaggacgt cgcggagatc ccgcttgaag atctcctgca atcgtcggtg atggatattt
    10381 accacacggc gcagcaggcg aaaattgacg tgcgactgac actcaatgtc cagggcatca
    10441 aacgcaccgg acaaccgcta ttgctaagtt tgttggtgcg aaatttgctg gataacgccg
    10501 tgcgttacag tccacagggc agcgtggtgg acgtcacgct gaatgccgat aatttcatcg
    10561 tgagggataa cggccccggt gtgacaccag aggcactggc gcgaattggc gaacgcttct
    10621 atcgcccctc cggacaaacc gctaccggca gtggacttgg gctatcgatt gtccagcgaa
    10681 tcgccaagct gcatggcatg aatgttgagt ttgggaatgc ggaacaaggt ggatttgagg
    10741 cgaaagtaag ctggtaaggg taagactttt gctaaattcg caaaagactt tgcacatttt
    10801 gctaatttca ccgtaccgct ctgtgacgta ctatagtcgg caaacgtctc accttgaggt
    10861 taaaaaatga gcaacattct gattatcaac ggcgcgaaaa aattcgccca ctccaatggt
    10921 caactgaatg acaccctgac cgaagtcgcg gatggcacac tgcgcgacct cgggcatgat
    10981 gttcgcatcg ttcgcgccga cagcgactac gatgtcaaag cggaagtaca aaactttctc
    11041 tgggctgatg tggtgatctg gcagatgcca ggctggtgga tgggcgcgcc gtggacagtg
    11101 aaaaaataca ttgatgatgt attcaccgaa ggtcacggga cgctgtatgc cagcgatggc
    11161 cgtacccgca aagatccgtc aaaaaaatac ggttccggcg gcctggtaca gggcaaaaaa
    11221 tatatgcttt ctctgacctg gaacgcacca atggaagcct tcaccgaaaa agatcaattc
    11281 ttccacggcg ttggcgtcga cggtgtgtat ctgccgttcc ataaagcaaa ccaattcctt
    11341 ggcatggaac cgctgccgac atttatcgct aatgacgtga taaaaatgcc tgatgttccc
    11401 cgctatactg aagaatatcg caagcatctt gtggaaattt ttggttaact agagctcagg
    11461 ctttagaagg agttaaccat gcttaccgta atcgcagaaa tccgtactcg tcctggtcaa
    11521 catcaccgtc aggcggtatt ggatcagttt gctaaaatcg ttccaaccgt actgaaagaa
    11581 gaaggttgcc acggctatgc gccaatggtg gattgcgctg ctggcgtgag tttccagtct
    11641 atggcaccgg attctatcgt gatgattgag cagtgggaaa gcatcgcgca ccttgaagcg
    11701 catctgcaaa ccccgcacat gaaggcgtac agcgaagccg taaaaggtga cgtgctggag
    11761 atgaatattc gtattctgca gccagggatt taatcctgcc ttgtttgccc ggccatcctg
    11821 accgggcaat gttctttcct ttaaacctca atctccgcca tgtcgccttt ctcttgcaac
    11881 cagttgcggc gatcttccga gcgtttcttc gccagtagca tgtccatcat cgcgtcagta
    11941 cgctgatcgt cttcatcatc gatagtcaac tgcaccagac ggcgagtgtt cggatcaagc
    12001 gtggtttcgc gcaattgcat cgggttcatt tcccccagac ctttaaaacg ctggacgttc
    12061 ggcttgcctt tcttgcgttt taattgctca agtacgccct ctttctcttc ttccgtcagc
    12121 gcgtaataaa cctctttccc gagatcaata cggtagagcg gtggcagtgc gacgtaaacg
    12181 tgaccgtgtt tcaccaacgc gcggaagtgt tttacaaaca aagcgcagag cagcgtggca
    12241 atgtgcagac catcggagtc agcatccgcc aggatacaga tcttgccgta acgaagctgg
    12301 ctcaaatcgt cgctgtcagg atcgataccg atcgctaccg aaatatcgtg cacttcctgc
    12361 gaagccagca cttcgtcgga agagacttcc caggtgttaa ggatcttacc tttcagtggc
    12421 atgatcgcct gatattcgcg atcgcgcgcc tgcttggcag atccgcctgc ggagtcacct
    12481 tccacaagaa acagttcggt gcggttaagg tcctgcgcgg tacagtcagc cagtttgcca
    12541 ggcagcgccg gaccgctggt cagcttttta cgcaccactt ttttagccgc acgcatacga
    12601 cgctgggcgc tggaaatcgc catctctgcc agcaactccg ccgcctgaac gttctggttc
    12661 agccacagga taaaggcatc tttcaccacg ccagaaacga atgccgcgca ctgacgcgaa
    12721 gagagacgct ctttggtctg cccggcaaac tgcggatcct gcatttttac tgacagcaca
    12781 taggcgcagc gatcccagat atcttccgcc gacagcttta caccgcgcgg cagaatgttg
    12841 cggtattcac agaactcacg catcgcgtcc agcaggccct gacgcagacc attaacatgg
    12901 gtaccgccct gcatcgttgg gatcaggttg acgtagcttt cggtcagcag ttcaccgcct
    12961 tccggcagcc acagtagcgc ccagtccacc gcttcagtat cgccagcgaa attaccgata
    13021 aacggttttt ctggcagcgt cggtaaaccg tttaccgctt ccgccaggta atcattcaga
    13081 ccgtcctgat agcaccagcg ctgttcggtg ttgttgatct catctttaaa ggtaatctca
    13141 acgccggggc acagtactgc tttggctttc agcacatgcg tcaggcgtga aacagaaaaa
    13201 cgcgggctat cgaagaaggt ttcatccggc cagaaatgga cgctggttcc ggtattgcgt
    13261 ttaccgcaag tgccgacaac ctgtaaatcc tgcacctttt cgccattttc aaaggcgatg
    13321 ttataaatct gaccatcgcg gcgcacgtta acttctacgc gcttcgacag ggcgttaacc
    13381 accgaaatcc ccacgccatg caggccgcca gagaactggt aatttttgtt agagaattta
    13441 ccgcctgcat gcagacggca aagaatcagt tcaaccgccg gtacaccctc ttccgggtga
    13501 atatccaccg gcatcccgcg cccatcgtca ataacttcta acgactggtc agcatgtaaa
    13561 ataacgtcca cgcgttttgc gtgacccgcc agtgcttcat ccacactgtt atcaatgact
    13621 tcttgcccca aatggttagg gcgagtggta tcggtataca tccccggacg gcggcgaacc
    13681 ggctcaagcc cggtgagtac ctcaatggca tcagcgttat aagtttgcgt catggtttaa
    13741 gttagtaatt cgagttgatc gtcagagatg gtgcagacca agaaaatcga cgatcgggtt
    13801 gaaataatct tcgaagcccg tgaatgcgtg gttgccgcct tctatgacag tctggcggca
    13861 ggaagcgtag tacgccaccg cctggcggta atccagcact tcatctcccg tctgttgcag
    13921 cagccagatc aaatccggcg cttccagcgg gtcaatctgc atgactttaa gatcgtaaat
    13981 atggcgtgac tctagcacat attgctgccc ggtgtagggg ttctcgttct gaccgagata
    14041 gtccgtcagc agttcaaacg ggcgcaccgc cgggtttacc accactgcgg gcagcataaa
    14101 acattgtgac aaccaggtgg cgtaatatcc ccccagtgac gaaccgacaa tacccagcga
    14161 atcgccgcca tgttccagga caatggattc cagcagctct gccgcgtcgg aaggatacgg
    14221 cggcaactgc ggaatgatca tctcaacgtc agggtgatgt tccgccagcc agttttttaa
    14281 caagctcgct tttgcagagc gcggcgagct gttgaaaccg tgtaaataaa gaagcgtaga
    14341 catcagtagc cttctgaagc ggtatcaggt tggaaacgtg tgtccgccag gcgatgcacc
    14401 tcggtggtca gcgtgccatc agcatgtaac tcgagagtac gccagccggg cgcgatggta
    14461 tccagcgtaa agttggaaca gtgcggctta aactgcacac aggtcgacgg cgttgccagc
    14521 aggcggcgac cgttccagtc gagatccagc tcctgatgaa tatgaccgca cagcaagtat
    14581 ttgacgtgcg gaaacttcgc cagcacggta tccagttcgc ccgcgttacg cagactgtgt
    14641 tgatcgagcc aactacaacc cgcaggtagc ggatgatgat gcagcagcag caacgtatgg
    14701 cgttctggcg catcggccag tttacgttcc agccactcaa gctgaaactc gctcagctca
    14761 ccgtgcggca cgccaaacac ctggctatcc agcaacagga tttgccattg ctcaccaata
    14821 aacacgcgct tcgccgggga gatacccgca tcctgtaacg cgctgtacat cgcgggctgg
    14881 aaatcgtggt tgcccggcag ccagacgcag ggcgcacgaa aacttgcgat gccttcagcg
    14941 aaatgctgat aggccgcaga ggattgatcc tgcgctaaat cacctgtcgc gacaatcagg
    15001 tcgaattcgt gctgatgtgg acgaatcgcc tccagcaccg cctggtagct ttcccaggtg
    15061 tttaccccta acagggcttc gtgcttttgt gcaaacaggt gagtatcggt aatttgtaaa
    15121 atcctgactc tggcctcacc agccagagga agggttaaca ggctttccaa atggtgtcct
    15181 taggtttcac gacgctagta aaccggaatc gccatcgctc catgtgctaa acagtatcgc
    15241 aaccagtccg ctaaaaactg gttaatttga tgcttttcgt cgcgttgatg caacttttta
    15301 ttaggataat cataccgcgc tttgaagcga aaaatctgct ggcttgaaca cacttcagcc
    15361 accatcgcgt catgatacag acgcaccgtc attgacggaa ggctccagta actgatcgcg
    15421 ggcgcagtct gttctattgt caccagggta gtgtatcggg tcgattccac aatcgtcagc
    15481 cgatattgtg cgtttgccac ctgatagctt acagtttcgc cgggtgcgtc attgcgcggt
    15541 aacaaacggc gcaattgtga aaagttcatc tcgcacaggc gcatcatttc aggaaagtca
    15601 ggtgtgtaac gcttcattta tgcccactca ttttttaacg cttggtggtg cagctgcagc
    15661 cattgcaaag cgatgaccga cgctgcgttg tcgattttcc cctcttctac ccactggtat
    15721 gcctgttccc ggcttaccac atgaacgcga atatcttcgt tttcatcagc cagaccatga
    15781 ataccgcttg cggtcgtggc gtccacttcg cccaccataa ttgacgaacg ctcactggtg
    15841 ccccccgggc ttgccaggaa acttaacacc ggtttggtcc gtttgactat cagccccgcc
    15901 tcttcaatcg cttcgcgacg ggcaacatct tccacacttt caccctcttc aatcatcccg
    15961 gcaaccatct ccagtagcca gggggtttcg ctagtgtcgt acgcggcaat tcgaatctgc
    16021 tcaatcagca caacttcatc acgcactggg tcaaagggta gcaagactgc ggcgtgaccg
    16081 cgctcaaaaa tttcccgccg cacctcatga ctcatttgcc cgttgaatag actatgacga
    16141 aatctataaa gatctaatga aaaaaagccg cgataaagtg tttctcgtgc aataatttct
    16201 acatcgtttt tgccaaatgt aacgggcagg ttgtctggct taagcattgt taatgtcctg
    16261 gcactaatag tgaattaaat gtgaatttca gcgacgtttg actgccgttt gagcagtcat
    16321 gtgttaaatt gaggcacatt aacgccctat ggcacgtaac gccaaccttt tgcggtagcg
    16381 gcttctgcta gaatccgcaa taattttaca gtttgatcgc gctaaatact gcttcaccac
    16441 aaggaatgca aatgaagaaa ttgctcccca ttcttatcgg cctgagcctt tctgggttca
    16501 gttcgttgag ccaggccgag aacctgatgc aagtttatca gcaagcacgc cttagtaacc
    16561 cggaattgcg taagtctgcc gccgatcgtg atgctgcctt tgaaaaaatt aatgaagcgc
    16621 gcagtccatt actgccacag ctaggtttag gtgcagatta cacctatagc aacggctacc
    16681 gcgacgcgaa cggcatcaac tcgaacgcga ccagtgcgtc cctgcagtta actcaatcca
    16741 tttttgatat gtcgaaatgg cgtgcgttaa cgctgcagga aaaagcagca gggattcagg
    16801 acgtcacata tcagaccgat cagcaaacct tgatcctcaa caccgcgacc gcttatttca
    16861 acgtgttgaa tgctattgac gttctttcct atacacaggc acaaaaagaa gcgatctacc
    16921 gtcaattaga tcaaaccacc caacgtttta acgtgggcct ggtagcgatc accgacgtgc
    16981 agaacgcccg cgcgcagtac gataccgtgc tggcgaacga agtgaccgca cgtaataacc
    17041 ttgataacgc ggtagagcag ctgcgccaga tcaccggtaa ctactatccg gaactggcgg
    17101 cgctgaatgt cgaaaacttt aaaaccgaca aaccacagcc ggttaacgcg ctgctgaaag
    17161 aagccgaaaa acgcaacctg tcgctgttac aggcacgctt gagccaggac ctggcgcgcg
    17221 agcaaattcg ccaggcgcag gatggtcact taccgacgct ggatttaacg gcttctaccg
    17281 ggatttctga cacctcttat agcggttcga aaactcgtgg tgccgctggt acccagtatg
    17341 acgacagcaa tatgggccag aacaaagtgg gcctgagctt ctcgctgccg atttatcagg
    17401 gcggaatggt taactcgcag gtgaaacagg cccagtacaa ctttgttggt gccagcgagc
    17461 aactggaaag cgcgcatcgt agcatcgtgc aaaccgtacg ttcctccttc aacaacatta
    17521 atgcatctat cagtagcatt aacgcctaca aacaagccgt agtttccgct caaagctcat
    17581 tagacgcgat ggaagcgggc tactcggtcg gtacgcgtac cattgttgat gtgttggatg
    17641 caaccaccac gctgtacaac gctaagcaag agctggcaaa tgcgcgttat aactacctga
    17701 ttaatcagct gaatattaag tcagccctgg gtacgttgaa cgagcaggat ctgctggcac
    17761 tgaacaatgc gctgagcaaa ccggtttcca ctaatccgga aaacgttgcc ccgcaaacgc
    17821 cggaacagaa tgctattgct gatggttatg cgcctgatag cccggcaccc gtcgttcagc
    17881 aaacatccgc acgcactacc accagtaacg gtcataaccc tttccgtaac tgatgacgac
    17941 gacggggctt cggccccgtc tgaacgtaag acaacgtaaa gatacgggtt atctgccgca
    18001 ttcttccccc ttctcgcttc aatttcgacc agccatcctc tattctgatg ggtatttacc
    18061 actggtcccg gaagacaaaa atgaaacgga caaaatccat acgccacgca tcgttccgca
    18121 aaaactggag cgcacgccat ctgacaccag tcgctctcgc ggttgccact gtttttatgc
    18181 tggctagctg tgaaaagagt gatgaaacag tgtctctcta tcaaaatgct gacgactgtt
    18241 cagctgcaaa cccaggcaaa agcgccgaat gtaccaccgc gtacaacaat gcgctgaaag
    18301 aagccgaacg tactgcgccg aaatacgcca cccgtgaaga ctgtgttgct gaatttggtg
    18361 aaggtcagtg ccagcaggca ccagcccagg ctggcatggc accagaaaac caggcgcagg
    18421 cccagcaatc cagcgggagt ttctggatgc cgctgatggc cggttacatg atggggcgtc
    18481 tgatgggcgg cggcgcggga tttgcacagc agccgctgtt ctcctcgaaa aacccagcca
    18541 gcccggctta cggtaaatat accgacgcga cgggtaaaaa ctatggcgca gcccagccag
    18601 gccgcaccat gaccgtaccg aagacagcca tggcaccaaa accggcgacc actactaccg
    18661 ttacccgtgg cggttttggt gaatctgttg ccaaacaaag cactatgcag cgtagcgcaa
    18721 ccggcacctc ttctcgttca atgggtggct gataccgatg gaaagagtca gtattaccga
    18781 gcgcccggac tggcgtgaaa aagcccacga atacggtttc aattttcaca ccatgtacgg
    18841 cgagccgtac tggtgtgaag atgcttacta caagttgccc ctcgcccagg ttgaaaagct
    18901 ggaagaagtc accgccgaac tgcaccagat gtgcctgaaa gtggtggaaa aagtgatcgc
    18961 cagcgatgag ctgatgacca aattccgcat tccaaaacac acctggagtt ttgtgcgtca
    19021 gtcatggctg actcaccagc catcgcttta ttcgcgtctt gatctggcat gggatggcac
    19081 tggcgaacct aaactgctgg aaaataacgc cgatacacca acgtcactat acgaggcggc
    19141 gttctttcag tggatctggc tggaagatca gcttaacgcc ggtaacttgc cggagggcag
    19201 cgaccagttt aacagtctgc aagaaaagct aatcgatcgc tttgtcgagt tgcgtgaaca
    19261 gtatggcttc cagttgctgc atctcacctg ctgtcgcgac acggtggaag atcgcggaac
    19321 cattcagtat ttgcaggact gcgcgacgga agctgaaatc gctaccgagt tcctctacat
    19381 tgacgatatc gggttaggtg aaaaaggtca gttcacggat ttacaggatc aggtaatttc
    19441 caacctgttc aagctgtatc catgggaatt tatgttgcgt gagatgttct ccaccaagct
    19501 ggaggatgca ggcgtacgct ggctggaacc ggcgtggaag agcattatct ccaacaaagc
    19561 gcttctaccg ctactgtggg agatgttccc gaatcacccg aacctgctgc ccgcttattt
    19621 tgcggaagat gatcatccgc aaatggaaaa atatgtggtt aaaccgatct tctcccgtga
    19681 aggcgcaaac gtgtcgatca ttgagaacgg caaaaccatt gaagcagcgg aaggtccgta
    19741 tggcgaagaa gggatgattg ttcagcaatt ccatccgtta ccgaaattcg gcgacagcta
    19801 tatgctgatt ggtagctggc tggtgaacga tcaacccgcc ggaattggca ttcgtgaaga
    19861 ccgtgcattg atcacccagg atatgtctcg gttttatcca catatttttg ttgagtaatc
    19921 aatgatgccg gatggcattc gccatccggt aattgttaac ctatctgcac cgacagcata
    19981 ctcaggctgc ccatttctat accatcgacc ggaatggtaa ttggctcctg cccatcccac
    20041 gcacctaaga catacaacaa cggcagatag tgctccggcg ttgggttcga taacgcgcca
    20101 ccttcatggt cgaggtaatt caccagagga tgctgctcta ccgccccttg ccacgtcaga
    20161 ttcgctttca catactcatt aaacgacatc gcccaaggat atggtgaact atcaccgtgc
    20221 cacttcactg tacgcaggtt atgcaccacg ttgccactgg cgaccaacat tattccttca
    20281 tctcgcagcg ctgccagttt gcgccccatt tcgaaatgcc aggcggcagg tttgctactg
    20341 tcgatactca actgcaccat cgggatatcg gcgtcaggat acatcttaat cagcacgccc
    20401 caggaaccgt ggtcaaagcc cctggcttct ttatccagcg tcaccgggac cggcgctaac
    20461 agctcaacca gatgctgtgc cagcacaggc gaacccggag caggataatg cgtatcgtac
    20521 agcgcctgcg ggaagccacc aaagtcatga atcgtgggcg gcgtctccat cgcggtcact
    20581 cctgttccac gggtaaacca gtgagccgaa accaccacaa tcgcttgcgg gcgtggcaat
    20641 gtcatcccca acttctgcca gctgcgggta tacaaattat cttccagcac gttcatcgga
    20701 ctaccgtgac ctaaaaacaa tgctggcata cgtgttgaag acatgatgat atccttaact
    20761 aagggtgtca ttttgatatc ctcacaatac gcttgttcgg cggagtaaga acccggataa
    20821 caatgatgat gatcatcagt tattttgacg atctgcctga aggtgaagat ttataaggag
    20881 ttgtcgatgt cagtacctct cattctgacc atactggcgg gggcagccac gtttattggc
    20941 gcgtttctcg gcgttctcgg acaaaaaccc tcgaaccgct tactggcgtt ttcgctaggt
    21001 tttgcggcgg ggatcatgtt gctcatctca ttaatggaaa tgcttcctgc cgcactagca
    21061 gctgaaggaa tatcgcctgt gttgggttat ggaatgttta tcttcggtct gcttggctac
    21121 tttggcctgg accgcatgtt gccgcatgcg catccgcagg atttaatgca aaaatcggtg
    21181 cagccgttgc caaaatcgat caagcgcaca gccattctgc tcactctcgg catcagtctg
    21241 cataacttcc cggaagggat tgccaccttt gtcacggcga gcagcaacct ggagctggga
    21301 tttggcatcg cactggccgt cgcgttgcac aatatccctg aaggtctggc agtggcaggc
    21361 ccggtttatg cggcaacggg gtctaaacgc accgcgattc tgtgggcggg gatttctgga
    21421 ctagcagaaa ttcttggtgg tgtgctggcg tggttaatcc tcggtagcat gatttccccg
    21481 gtggtcatgg cggcggttgc aggaattatg gtggcgctct cggttgatga attaatgccg
    21541 ctcgccaaag agattgaccc taataataac cccagctatg gcgtactatg tggaatgtca
    21601 gtgatgggat tcagtttagt gctgctacaa acggcgggaa ttggttaaaa acaacatcag
    21661 aagttgtcca gcacatggac aacttctgcc agcttcacga aagccttatt ctaccttttc
    21721 ggctgtctct tctctcgtac tgtttaagta tttgttccgc gcttcaagca gtgcgtctct
    21781 gtcctggcga aacgcccgac aataacgttt caaatgcagg aaataaccca ccacaaccag
    21841 agcaatcaat acaatccagt accaggcgat aaacatcctt tacccttaat atttaacaat
    21901 actaaacatt tagcgcataa atttcacata tcctttttcg gatatattca tatggtcgca
    21961 taataacgga acaattatat gattttgatc ataatgaaaa agaatgcctg atattaatga
    22021 aattttctca tagaaagaga agcggaatta acatcttgca tagcagggca aaataaaaac
    22081 cgaagccagc tacaacttcg gtttcttgat taaggcagta attaagcagc ggttttcagc
    22141 tggctttacg ctcatgtgcc tgacggtatg ccaccaggtc ttcaatagtc acgagcgcca
    22201 tattgtgttt attggcaaac tcaatgcact ctggtgcacg cgccatcgtg ccatcgtcat
    22261 tagtcagctc acacagcaca ccagccggtt taaagcctgc cagcgtcatc aaatcaatag
    22321 tcgcttcagt atgaccgcca cgcgtcagta caccacctgc ctgcgcgcga agtgggaaaa
    22381 cgtggcccgg gcgattcaga tcagaaggtt ttgcgccatc ggcaatcgct gcgcgaacgg
    22441 tcgtaatacg atcagcggca gaaacaccgg tagtcacacc ttcagccgct tcaatggtca
    22501 cggtaaaacc ggtgccatag gcgctggtgt tattttctac catcattggc agatcaagtt
    22561 gtttacggcg atcttcagtg atgcacaggc aaacaatacc gctaccgtgg cgaatggtca
    22621 gcgccatctg ctcaacggtc atggtttctg ccgggaagat catatcgcct tcgttttcac
    22681 ggtcttcatc atcaagcacc attacaccgc gtccttcacg cagcgcagcc agtgcatttt
    22741 caacacgttc gaaaggcgta ccaaaagagg aaagtagcgt ctgattcatg gtaaaaaaaa
    22801 ctcactaaaa ttatggttac cagaatcagg gcagtcttag gagtggcggc atatagccaa
    22861 aataacgtga gcgggtccat gcccgacaga atcgttactc tctcccatcc ggactctaac
    22921 cgtcggcccc ggaattacac cggatctgct gtcctttgag ttcgcaccca aagcgctcgc
    22981 gggctttcaa ctgagttgat ttaccgccgg tggggaattt cgccccgccc tgagaataag
    23041 cggattcact ataacgctaa tgattagcgg cagcaacgca tagcttcaca taattctggt
    23101 ttatgactta cccttatcgc actacaatgg tactcaacac ctatcagtac agggaaacta
    23161 caatgattga cccgaaaaaa attgagcaaa tcgctcgcca agttcacgaa tcaatgccta
    23221 aaggaatcag ggagttcggg gaagatgtgg agaaaaaaat ccgccaaacc ctacaagctc
    23281 agctgacgcg cctcgacctt gtaagccgtg aagagttcga cgtccaaacg caagtcctgt
    23341 tacggactcg tgagaaactg gcgctgcttg aacaaagaat gagcgaactg gaaaatcgca
    23401 gtactgagat taaaaagcag ccagatccag agactctccc cccaacgctg taaacatttg
    23461 tctttaatgc actgtaaatt atgaattaat cttctaattt acagtgcttt ctattacctt
    23521 atcgcttaat tccattcaga atatcaccac aaaaatgaat gagtgcaatt gaaaatataa
    23581 ttgttatgta tttttttaag ttttacttaa tccatattta gataaactaa tttcataaaa
    23641 cccgaaaagt aaaatataat atttgaatga ttagcaatcc ttaaataaca gttactaatc
    23701 attcattttc tacttatatt tatataaagg aatattatgt ctgcttttaa aaagtcactt
    23761 cttgttgctg gcgtagcaat gattctttcc aataacgtat ttgctgacga aggtcacggc
    23821 attgttaaat ttaaaggtga agttatctct gctccatgtt caattaaacc aggtgacgaa
    23881 gacttaactg taaatctggg tgaagtagca gataccgttc tgaaaagcga ccagaaatct
    23941 ttggctgagc ctttcactat tcacctgcaa gactgcatgt tgagccaggg tggaaccact
    24001 tattcaaaag cgaaggttac tttcaccaca gcgaatacta tgacgggtca gactgacctg
    24061 ctgaaaaaca ccaaagaaac tgaaatcggt ggtgccacag gcgttggcgt acgtattctg
    24121 aatagccaga gcggcgaagt gactttaggt actccggtag taattacatt caacaatact
    24181 aacagctatc aggaactgaa cttcaaagtt cgtatggaat ccccgggcaa agatgctacc
    24241 ccgggcaacg tttacgctca ggctgactat aagattgctt acgagtaatc acattaggcc
    24301 gggttcgccc ggccacatca ttaacggatt aatgataagt ggaacagatg tataaaaaat
    24361 taaaattaac cacaataagc gaattgatta aaaatattta ttgttcatta tccgttctca
    24421 tcattggttg tgcgtcagct tatgccgttg aattcaacaa agatttaatc gaagccgaag
    24481 atcgtgaaaa cgttaacctt tcccaatttg aaactgatgg ccaattaccc gtaggtaaat
    24541 actcattaaa cgctattatt aataataaaa ggacgccaat ccatctggac ctgcaatggg
    24601 tattaattga taaccaaact gcagtttgtc tgactccaga gcaattaaca ttattaggct
    24661 ttactgatga gattattgaa gaggctcagc aaaacctgat cgatggttgt taccctatcg
    24721 aaaaagaaaa acaaattaca acttatctcg ataaagggaa aatgcaatta tccatatctg
    24781 cacctcaggc atggttaaaa tacaaagatg caaactggac gcc
//
LOCUS       DAJTMC010000047        24755 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.047, whole genome shotgun sequence.
ACCESSION   DAJTMC010000047 DAJTMC010000000
VERSION     DAJTMC010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24755
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.047"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(<1..224)
                     /locus_tag="OO130_001696"
     CDS             complement(<1..224)
                     /locus_tag="OO130_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2170 family protein"
                     /protein_id="HCR5365151.1"
                     /translation="MTWNLLALATALQTVPEQNIDVTNSENALIIKMNDYGDLQINIL
                     FTSRQMIIETFICPVSSISNPDEFNTFLLR"
     gene            complement(351..1082)
                     /gene="rlmB"
                     /locus_tag="OO130_001697"
     CDS             complement(351..1082)
                     /gene="rlmB"
                     /locus_tag="OO130_001697"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HCR5365152.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     AQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLVRT
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(1263..3704)
                     /gene="rnr"
                     /locus_tag="OO130_001698"
     CDS             complement(1263..3704)
                     /gene="rnr"
                     /locus_tag="OO130_001698"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HCR5365153.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAECRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRLVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3743..4168)
                     /gene="nsrR"
                     /locus_tag="OO130_001699"
     CDS             complement(3743..4168)
                     /gene="nsrR"
                     /locus_tag="OO130_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HCR5365154.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(4373..5671)
                     /gene="purA"
                     /locus_tag="OO130_001700"
     CDS             complement(4373..5671)
                     /gene="purA"
                     /locus_tag="OO130_001700"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HCR5365155.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5775..5972)
                     /locus_tag="OO130_001701"
     CDS             complement(5775..5972)
                     /locus_tag="OO130_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HCR5365156.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(6054..7058)
                     /gene="hflC"
                     /locus_tag="OO130_001702"
     CDS             complement(6054..7058)
                     /gene="hflC"
                     /locus_tag="OO130_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HCR5365157.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(7061..8320)
                     /gene="hflK"
                     /locus_tag="OO130_001703"
     CDS             complement(7061..8320)
                     /gene="hflK"
                     /locus_tag="OO130_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HCR5365158.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTYPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8406..9686)
                     /gene="hflX"
                     /locus_tag="OO130_001704"
     CDS             complement(8406..9686)
                     /gene="hflX"
                     /locus_tag="OO130_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HCR5365159.1"
                     /translation="MFDRYDAGEQAVLVHIYFTKDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETILADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9763..10071)
                     /gene="hfq"
                     /locus_tag="OO130_001705"
     CDS             complement(9763..10071)
                     /gene="hfq"
                     /locus_tag="OO130_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HCR5365160.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(10157..11107)
                     /gene="miaA"
                     /locus_tag="OO130_001706"
     CDS             complement(10157..11107)
                     /gene="miaA"
                     /locus_tag="OO130_001706"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HCR5365161.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAITG"
     gene            complement(11100..12947)
                     /gene="mutL"
                     /locus_tag="OO130_001707"
     CDS             complement(11100..12947)
                     /gene="mutL"
                     /locus_tag="OO130_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HCR5365162.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMNVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRSIPENRVAAGRNHFAEPAAREPVAPRYTPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKLKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLALPVAERWLRQVQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12957..14294)
                     /gene="amiB"
                     /locus_tag="OO130_001708"
     CDS             complement(12957..14294)
                     /gene="amiB"
                     /locus_tag="OO130_001708"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HCR5365163.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKHSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(14313..14774)
                     /gene="tsaE"
                     /locus_tag="OO130_001709"
     CDS             complement(14313..14774)
                     /gene="tsaE"
                     /locus_tag="OO130_001709"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HCR5365164.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14746..16293)
                     /gene="nnr"
                     /locus_tag="OO130_001710"
     CDS             complement(14746..16293)
                     /gene="nnr"
                     /locus_tag="OO130_001710"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HCR5365165.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLFAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLRQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHSITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            16292..17431
                     /gene="queG"
                     /locus_tag="OO130_001711"
     CDS             16292..17431
                     /gene="queG"
                     /locus_tag="OO130_001711"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HCR5365166.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQPICPWNRYSQLTTEEDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGGH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17414..17467)
                     /locus_tag="OO130_001712"
     CDS             complement(17414..17467)
                     /locus_tag="OO130_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5365167.1"
                     /translation="MRFYFYSQAVDESGVTR"
     gene            complement(17696..17771)
                     /locus_tag="OO130_001713"
     tRNA            complement(17696..17771)
                     /locus_tag="OO130_001713"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17736..17738),aa:Gly,seq:gcc)
     gene            complement(17808..17883)
                     /locus_tag="OO130_001714"
     tRNA            complement(17808..17883)
                     /locus_tag="OO130_001714"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17848..17850),aa:Gly,seq:gcc)
     gene            complement(17920..17995)
                     /locus_tag="OO130_001715"
     tRNA            complement(17920..17995)
                     /locus_tag="OO130_001715"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17960..17962),aa:Gly,seq:gcc)
     gene            complement(18206..18751)
                     /gene="orn"
                     /locus_tag="OO130_001716"
     CDS             complement(18206..18751)
                     /gene="orn"
                     /locus_tag="OO130_001716"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HCR5365168.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDSFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            18846..19898
                     /gene="rsgA"
                     /locus_tag="OO130_001717"
     CDS             18846..19898
                     /gene="rsgA"
                     /locus_tag="OO130_001717"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HCR5365169.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            19995..20963
                     /gene="psd"
                     /locus_tag="OO130_001718"
     CDS             19995..20963
                     /gene="psd"
                     /locus_tag="OO130_001718"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HCR5365170.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            20985..24308
                     /gene="mscM"
                     /locus_tag="OO130_001719"
     CDS             20985..24308
                     /gene="mscM"
                     /locus_tag="OO130_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HCR5365171.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERTREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(24459..>24755)
                     /locus_tag="OO130_001720"
     CDS             complement(24459..>24755)
                     /locus_tag="OO130_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002459969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5365172.1"
                     /translation="LPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIATVVVVLVVT
                     FANVFTIIPPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
BASE COUNT         5697 a         6912 c         6292 g         5854 t
ORIGIN      
        1 tttcttaata agaaggtatt aaattcatct ggattgctga tactacttac cggacaaata
       61 aaggtttcga taatcatttg gcgggaagta aaaagaatat tgatttgcaa atcgccataa
      121 tcattcattt taataattaa tgcgttctcg ctatttgtta catcaatatt ttgttcaggt
      181 acagtttgca gcgccgtcgc cagcgccaac agattccatg tcatatatta ttccatatag
      241 atttagttta aatattaata aaatgaatat ttgcagtacg taattatctt accagctata
      301 gacaaaaaaa aaccatccaa atctggatgg cttttcataa ttcagaggaa ctagctgcgc
      361 tgacgcaccg cttcaaataa gcaaattccg gttgcaaccg aaacgttcag agaagaaaca
      421 cttcctgcca ttgggatgct gatcaactca tcgcaatgtt cacgggtcag gcgacgcata
      481 ccttcacctt ccgcgcccat caccagcgcc aggcgtccgg tcattttgct ctgatagagt
      541 gtatgatccg cctcacctgc cgtaccgacg atccagatat tctcttcctg caacatacgc
      601 atggtacgca ccaggttagt cacccgaatc agcggaacgc tttctgccgc gccacaggcc
      661 acttttttcg ccgtggcgtt gagctgggcg gagcgatctt tcggcacaat caccgcatga
      721 acaccagcgg catctgcgct acgcaggcac gcgccgaggt tatgcggatc ggttacaccg
      781 tcaaggatca gcaggaacgg ctgatcgagc gaagcgatca gatccggcaa atcattttcc
      841 tggtactgac gtcctggctt cacgcgggcg ataatgccct gatgcacggc accgtcgctt
      901 ttctcgtcga gatattggcg gtttgccagc tggataacca cgccctgggc ttcgagggcg
      961 tgaatcagcg gtaacagacg tttatcttca cggcctttta aaataaagac ttcctgaaaa
     1021 cgttcagggg cgcgctccag cagggcctgc actgcgtgga tgccgtaaat catttcgctc
     1081 attaatgtac tcgttgttta cgttttgcct gatgcactac gtttatccgg cctacatgat
     1141 ctctgcaata tattgaattt gcgtgattat gtaggccgga taaggcgttc acgccgcatc
     1201 cggcatgaac aaagcgcatt ttgtcagcaa tctaaccctc ttctgtttga agagggtaat
     1261 gatcactctg ccactttttt cttcgccgca cgcttcgctt tggtcgctgc agctattttc
     1321 tgcgttttcg ccgatggctt tttcgctttt ctcgcgtctt tcttcgctgc tttcggcttc
     1381 gtttttttct caccgcggaa ggcgctgtct ggctcaaagt ttaccttttt accgaccaga
     1441 cgacgcttgc cgcctttttt acctgcatcg ccttttttcg ctttctcgcg cgccgtttta
     1501 ccgacgttgc gcggtgcgcg ttcgctggag atcaggctaa agtcgatttt gcgctcgtcc
     1561 atattaaccg cttcgacgcg aacttccacg cgatcgccca gacgataagt ctggccgctg
     1621 gattcaccca tcagccgttg ccctacctgg tcaaagcgat agtagtcatt gtccagcgaa
     1681 gagacatgga ccagaccatc aatgaataag tcgtccagac ggacaaagaa tccaaagcca
     1741 gtgacgctgg aaattacgcc tttaaagacg ttacctacct ggtcgagcat gaagtcacac
     1801 ttcagccagt cagccacatc gcgcgttgct tcgtcggcac gacgttccgc catcgaacag
     1861 tgctgaccca gttgcagcat ttcttccatc gaataatggt agccgccggt ttcagtggtg
     1921 ttgccctgat gcccctgctc tttcgccagc agatatttaa tggcgcggtg cagcgtcagg
     1981 tctggataac gacgaatcgg cgaagtaaag tgcgcatagg actgcaatgc caggccaaag
     2041 tgaccacggt tttctggatc gtaaatcgcc tgtttcatcg agcgcagcag catggtttgc
     2101 agcatttctg catcaggacg atcggcaacc gactccagca gctccgcgta gtcacgcggt
     2161 tccggcttgt taccgcccgg cagttccagc cccagctccg ccagtactga acggaaagag
     2221 gtaatcgctt cggtgctcgg cttgtcgtga atacggaaca atgctggttc tttggctttc
     2281 tcaacgaaac gcgccgccga gatattcgcc agaatcatgc actcttcaat taacttgtgt
     2341 gcgtcgttac gctgagtctg ttcgatacgc tcaatacggc attcagcgtt gaaaataaac
     2401 ttcgcttctt cgctctcaaa tgagatcccg ccacgttctt cacgggcttt atccagcact
     2461 ttatagaggt tatgcaactc ttcgagatgc ttaaccagcg gagcgtactg ctcgcgcaga
     2521 tcctgatcgc cctgcagaat atgccagact ttggtgtagg tcagacgcgc gtgagagctc
     2581 atcaccgctt cgtagaattt gtagcccgtc aggcggcctt tcgacgaaac cgtcatctcg
     2641 cacaccatac acaggcggtc tacctgcgga ttgagcgagc acagaccgtt agagagcact
     2701 tccggcagca tagggataac ctgcgaaggg aagtacaccg acgtaccacg attacgcgct
     2761 tctctatcca gcggcgttgg cggacgcaca tagtagctga cgtcggcaat cgcgacccat
     2821 aaacgccagc cgccgccgcg ttttttctcg cagtaaaccg catcgtcaaa gtcacgggcg
     2881 tcttcgccat caatggtgac cagcggtaaa tcgcgcaaat cgacacggcc cgcttttgct
     2941 tcttccggca cttcttcttt cagcccggca acctgttgct caacagcctg cggccagatg
     3001 tacggaattt catgggtacg caaagcgata tcaaccgcca tgccggtgcc catattgtcg
     3061 cccagcactt cgacgatttt acccaccgct ttggtacggc gagtcgggcg ttgggtcagt
     3121 tcaacaacca ccacaaagcc catccgcgcg cccatgatct gatcgggcgg gattaagata
     3181 tcgaagctca gacggctatc gtcaggaacc acaaagccga cgcccgcttc ggtaaagtag
     3241 cgaccaacaa tctggctggt ttttggcacc agtacgcgga caatacgcgc ttcacgacga
     3301 cctttacggt cagcacccag cggctgagcc agcacctgat cgccatgaat gcaggttttc
     3361 atctgctcgc tggagagata caaatcatct ttacgccctt caacccgcag aaagccgtag
     3421 ccatcacggt ggccaataac ggtacctttc accaggtcga ggcgttccgg cagcgcatag
     3481 cactgacgac gagtgaagac cagttgacca tcgcgctcca tcgcgcgcag gcggcgacgc
     3541 aggccttcaa gctgctcttc gccttcaatg tgcagttcta ccgccagctc atcacggctg
     3601 gccggttttt cacgtttggt taaatgttcg aggataaatt cccgactagg gatgggattc
     3661 gcgtattttt cagcttcgcg ttcctggaaa ggatcttgtg acatctcggt tcctccgttg
     3721 tcatctctga tgaagatttt cgtcactcca ccagcaataa tttataaagc ggttgattct
     3781 cttcaaccaa atcggcaagc gtgtagttat ccagttccgt aagaaaactt tgcacggcct
     3841 tagaaagcgc ctgtttcaac cgacaggcag gtgtaatgtg gcaaaactca ctgctgcaat
     3901 tcaccagcga taagggctcc agctcgcgca ccacatcacc aatacgtatc gcactcgccg
     3961 gtttacccag gcgaatgccg ccattttttc cacgaacagc agtcacgtag ccggcacgac
     4021 taagttgatt gattattttg accatatgat tacgggagac gccgtagacg tcagtcactt
     4081 cagaaatact ggtcatccgc ccttctggca atgacgccat gtagatcagc gcacgtaatc
     4141 cgtaatcagt gaaactcgtt aactgcacat caacctcaaa agggaaatcg ggaaaaaata
     4201 catttatatt gatgataaac cagccacaag ctgtgtcgct aatttatttc agttcgggga
     4261 aggaaaaaag cgaggattta acactgtgtc ggatagcgta atggcttaac cgacacagca
     4321 atgtatcaca ctgttagccc ggcaggcaaa atatctgcca ggcgtaccag aattacgcgt
     4381 cgaacgggtc gcgcagaatc atagtttcag tacgatccgg accggtagag atgatatcga
     4441 tcggcacacc ggtcagctct tcaatacgct tgatgtagtt cagcgccgcc tgcggcagcc
     4501 cgctacgatc tttcacgccg aaagtggatt cagaccagcc cggcatggtt tcgtaaatcg
     4561 gctctacacc tttccagtcg tcagctgcca gcggagtggt agtcacttcg cgaccatccg
     4621 gcatacggta agccacgcag agtttaacct ctttcaggcc atccagaacg tccagtttag
     4681 tcaggcagaa gccagacagg gagttcagct gtaccgcacg acgaacggca acggtgtcca
     4741 gccagccggt acgacgacga cgacccgtcg ttgcgccgaa ttcgttaccc tgcttgcaga
     4801 ggaactcgcc agtttcatca aacagttcgg tcgggaaagg acctgcaccc acacgagtgg
     4861 agtaagcttt gaggataccc agaacgtaat caacataacg cgggcccagg ccggaaccgg
     4921 tcgccacgcc accagcagtg gtgttggaag aagttacgta cggataagta ccgtggtcga
     4981 tatccagcag cgtaccctgc gcaccttcaa acatgacgaa atcgccacgc tgacgcgcct
     5041 ggtcgagcag gtcagaaacg tcaaccacca tagaagtcag gatgtcggca acagccatcg
     5101 tatcatccag aactttctgg taatcaaccg cttcagcttt gtagtagtta accaactgga
     5161 agttgtgata ttccatcact tctttcagtt tttcagcgaa ggtttctttg tcgaaaaggt
     5221 cgccaacacg cagaccgcga cgtgccactt tatcttcata agcaggcccg ataccacgac
     5281 cggtggtgcc gatcgctttc gcgccacgcg ctttctcacg cgcgttatcc agcgcaacgt
     5341 gataatcaag gatcagcgga catgcttcag acagcagcag acgctcacga acggggatgc
     5401 cacggtcttc cagttctttc atctctttca tcagcgcggc cggagacagc acaacaccgt
     5461 taccgatgat gctggttaca ttctcgcgga gaatacctga tggaataaga tggagaacgg
     5521 ttttttcacc gttgattacg agagtatggc ctgcgttgtg accgccctgg tagcgtacaa
     5581 catatttagc ccgttcagtc agaagatcga cgatcttacc tttaccttcg tcaccccatt
     5641 gggtgcccag tacgacgacg ttgttaccca tttttcaaaa tcaccgtttg cttaaaaatg
     5701 gattctacca tcgctttttc agagttacag cactttttgc actcaaaaat gacctcgatc
     5761 agtctgtttt ttgttcagcc aatcgttttc ctcaacatgt agtagaccac aacgcccgca
     5821 accacaagtc caccgccaaa acgacgtaaa atattatcgg gcaaattggt catcgcagag
     5881 atcatcttct tccatgcctt cgggtaaagc atcggcccta aaccttccag taccaaaacc
     5941 agggcaagcg ccagccagat tgtcgaattc attcattatc cttatagaaa aagaaaacca
     6001 ccgacatccc tgaggatgcg gtggctttat tgacctgtac cgcagtcgtt atattaacgc
     6061 gttgcggaag tcggcgtctt catgtagcgg aagaaatcgc tatccgggct catgaccatc
     6121 acgtcctgat tgccagagaa gctgttctca taagcacgca ggctacggat gaatgcgtag
     6181 aagtccggat ctttactgaa tgcatcagca aacagtttgg ctgcttcggc atcgccttca
     6241 ccacgcatga tgcggccctg acgctcagct tctgccagcg ttctggtcac ttcatagtcg
     6301 gcagtcgcgc gcagtttttc cgcttcttcc tgaccttgtg aacggtgacg acgcgctacc
     6361 gcttcacgct cggcgcgcat acggttgtag atcgcttcag acacttcggt cggcaggttg
     6421 atctgcttga tacgcacatc gacaacttca atacccagcg ccgccatact gttcgggttg
     6481 atgaccggaa ctttgccctt cgtctcagcc gttacgcgct ctgccgcttc ggcaatggcg
     6541 ttatctgctg ccggggtagt aacttcatct tctgtacccg cagaaccgga gttcagcgcg
     6601 tcacgtactt cgagggtcag acgaccacgg gaatcagtga cgatatcttt cacgtccagg
     6661 cgaccaattt cagaacgcag acggtcagag aacttacgtt tcaacagcac ttccgcttgc
     6721 gaaatgtcgc caccacccgt tgccaggtag taacggctga aatcgctgat gcgccatttg
     6781 atgtaagagt cgacgatcag gtctttcttc tctttggtca caaagcggtc ggcctggttg
     6841 tccatggtct gaatacgtgc gtcgagcatt ttcaccgttt caatgaacgg tatcttgaaa
     6901 tgcagacccg gctcataaac cagaggtttg ttgtcatcgt cacgcagtac cttaccaaaa
     6961 cgcagcgtaa taccgcgctc accttctttg acgacaaaga cagacatgta aagcactacc
     7021 agcacgatga tgataatcgc gataactgac ttacgcatcg ttattccccc tgacgctggt
     7081 agtcgttacg ctgcgcgttg gcgcggcgtt ggtccataat atcgccctga ctggtggacg
     7141 acgtgttgct tgctccactg gttgtggagg aagaggctgg cggcaaacgc agcagattgc
     7201 tggcaccgtt atcgctcttc gccgcagggg cgttaccacc tttcagcatc tggtctaacg
     7261 gcagaaccat caggttgcca cctttatcgt taaccagcac tttgcgggtg ttacccaaca
     7321 ctttttccat cgtctcgata tacagacgct cgcgagtaat ttccggcgcg gctttatatt
     7381 ccggcagaag tttagcaaag cgcgccactt caccctgagc ttccaggatg gtctgggcct
     7441 tgtacgcacg cgcctcttcg aggatacgtt gcgcctgacc gttcgcacgc ggctgaactt
     7501 cgttggtata cgcttctgct tcacgaatgt attgctgttc gttttcacgc gcggcaatcg
     7561 catcgtcaaa cgccgctttt acttcttccg gcggacgagc agcctggaag ttgacgtcca
     7621 gcagagtgat gcccatgtca tacggacgaa tcgtctcttc cagttcgcgc tgggtatcgc
     7681 tacgaatcac ggtacgacct tccgtcagaa tgcggtccat ggtgtattta ccgataaccc
     7741 cgcgcagggc gctgtcggta gcctgacgca ggctgtcatc cgggctggtc acgctataca
     7801 gatatttttc cggataggta acgcggtact gcacgttcat ctcaacgcgc actacgttct
     7861 cgtccgacgt cagcatcaca ccggaagcgg ccagttcacg cacggcttcc acgtttaccg
     7921 gtttgacttc gtcgataaac gtcggtttcc agttcagacc cggctcaacc agatggctga
     7981 atttaccaaa gcgtgttacc acgccgcgtt cggcttcttt aatggtatag aaaccactgg
     8041 ccgcccagat aatgacaatc gctgccgctg cgatggtaac gacacgaccg ccaagctgcg
     8101 ggcgcgggcc ttgcgatgaa ctgccaccgc cagatccggt gcctttaccg ccgcccagac
     8161 caccgagctt tttgctcagt ttgcggaaga tatcatctaa atcaggtggc ccttgatcgc
     8221 gaccgccttt gtttccattt ccctcagagt tgccgccagg tttgctgctt ccccacgggt
     8281 cgcggtcttg tccgttatta ccgggctgat tccacgccat gtttgtgctc catatttgtt
     8341 atgcggtgat ccctgttgtc tttcgacgcc agaggatatg actccacgct tcagacgcta
     8401 cgccgttaga tcaggtaatc gatcaacgcc ggttcttgtt tacagaggcg acgccagtca
     8461 acgatcggca tacgaacttg cagacttacg ctgccgtcct cctccatcca ctctttttct
     8521 attgcctgaa gctgataaaa acgacttctc agacgccctt cctgcggtgg cagacgcaat
     8581 gtatgctgcg ccacctcgcc ggaaagccgc tccgtcaaag cctgaaaaag ctgtggtatc
     8641 cccgctccgg tctgtgcgga aagccagaca cggatcggtt tgttctcttc gtcccgatca
     8701 atacgcggtt cgaaatcttc cagcatatcg atcttgttca tcaccagcag ggttgggatc
     8761 tcgtgagcgt cgatctcttc aagaaccgta ttcaccgctt cgatgttttc ttgtacacgc
     8821 acatccgccg catcaatgac gtgcagcagt aatgtggctt gccgcgtctc ttgtaacgtg
     8881 gctttaaatg ccgccaccag atcgtgcggc agatggcgaa taaaccctac ggtatctgca
     8941 agtatggttt caccgacatc cgcgacgtca atacgccgca acgtcgggtc gagggtggca
     9001 aacaactggt ctgccgcgta gacccgcgct tcggtgatgc gattgaaaag ggtagattta
     9061 ccggcgttgg tatatcccac cagcgaaaca gtaggaacgt cggctttgat acgcgattgc
     9121 cgcccctgct cacgctgctt ttcaactctt tccaggcgcg actgtatctg cacgatgcga
     9181 ttacgcaaca aacgacggtc ggtttcgagc tgggtttcac ccggaccacg caaacctatc
     9241 ccgcctttct gtctttcaag gtgggtccag ccacgcacca ggcgcgtagc cagatggcgc
     9301 agctgcgcca gctcaacctg caacttaccc tcatgggtac gcgcacgttg ggcgaaaata
     9361 tctaaaataa ggccggtgcg gtcgatgaca cgacactcgc acaaacgctc caggttacgc
     9421 tcttgcgccg ggctcagggc atggtcaaaa agaacgaccg aagcacccgt cgctttgaca
     9481 gcttccgcaa tttcaactgc tttaccttca cctacaaaat actttgggtg cggcgcttta
     9541 cggctaccgg taatcacctg caatgcttcg acaccggcgg aagaaaccag agattcaaac
     9601 tcctggaggt cttccatatc tttgtctttc gtaaaataga tgtgtaccag taccgcctgc
     9661 tcaccagcat cataacggtc aaacaagcgt ataaccctct aaatagatca gcggggaacg
     9721 caggatcgct ggctccccgt gtaaaaaaaa cagcccgaaa ccttattcgg tttcttcgct
     9781 gtcctgttgc gcggaagtat tctgcgcgct gctaccatga tggtagttac tgctggtacc
     9841 gccaccggcg ttgttactgt gatgagaaac cggacgagac gggacaacag tagaaatcgc
     9901 gtgcttgtaa accatctggc tgaccgtgtt tttcaacagg atcacgaact gatcaaaaga
     9961 ctcgatttgc ccttgcagct taataccatt caccaaataa atagaaactg gaacacgttc
    10021 ccgacgcagt gcgttcagga acggatcttg taaagattgc cccttagcca ttctctcttt
    10081 tccttatatg cttatttgta ctttgaacct ttcgattctg aaaaaattgc gcacgatacg
    10141 tctcaattgt acacattcag cctgtgatag caccaacaac ctgtaatact tcgtcacgcg
    10201 cctgttctgg tttttcactg tcaagccagt gaaccccttc ccaaccacgc agccaggtta
    10261 tctgccgctt cgccaactgt ctcgtggcgc aaacacctcg ataaaccatt tcatcgtatg
    10321 agatttcgcc ttcaaggtaa gaccacatct ggcgataacc cacgcaacga atggaaggca
    10381 agtccgtatg caaatctcct cgggcaaaaa gcgcccggac ttctgcttca aaacctgaag
    10441 ccaacatctg atgaaaacgc tgctcaatgc gttgatggag cagttcacgg ctcgccgggg
    10501 cgatggcgaa ctgatgcacc tgatacggta gagcgtctcc tgacgtttgc gtcagttccg
    10561 ttaaagtttt acccgaaatg aaaaaaactt ccagtgcccg ggaaagcctt tgtggatcat
    10621 ttggatgaat ccttgctgcc gcaaccggat ctacctcctg aagttgacga tgcaatgact
    10681 cccaaccttg ctctgccgcc tgttgctcaa ttctggcccg cacttccggg tctgccgacg
    10741 gtagcggcga caacccttcc agcaatgcct tgaaatacaa catcgtaccg cccactaaca
    10801 gtgggatccg ccccgccgcg gtgatatcgg ccatttccgc cagcgcatcg cggcgaaaat
    10861 cagcagccga gtaagcctgc gacggatcgc gaatatccag caatcggtgc ggcgcggcga
    10921 gtaactcttc agcgttcggc ttcgccgtcc cgatatccat ccctttgtaa ataagggcag
    10981 aatcaacgct tatcaactct actggtaaaa ttttacgcag ctcaatggct aacgccgttt
    11041 taccggaggc cgtcggcccc atcaaaaaaa tcgccttagg caggctcgcc ttactgatat
    11101 cactcatctt tcagggcttt tatcgccgga tgtaaatcaa cagattgtaa cagaccaccc
    11161 ggcggcgttt tcacaagttg cggacataac cgttccacgt ccgccagcag ggttatggcc
    11221 tgtgccattg accactgcgc atgttcgctc atcagatttc gtgcaatcca ctgcgcaata
    11281 ttgccaggtt cgaatacgga ctgcttcgcc aggtagccta tcagttcagg aatcaagatt
    11341 tgtaaatttt gttggcgtaa gggtaaaggc acggccctga tggtcacatg ctgtgcatct
    11401 gactggaaat caatacccaa ttccgccagg gcagactgcg ctttttctaa tgccgatttt
    11461 tcttcgccag aaacttttag ccgcaacgga ataagcagcg gctgagcgca aactggcgct
    11521 tcacccgggg tcagttgtac ctgacgcagc caacgttctg ccactggcaa ggctaaaagt
    11581 gaaatgttgc cgtcgcgctc cagcaacgca cagtcggaat ggacgatagt cagtacccga
    11641 ccaaaactct gactgttcgc cgcaagtgca ggttcctgcg gttccggcgc ttttaatttt
    11701 tgcatcggcg cgggcgtttg caaaagctgg cgatacactt caccttgctg tttctggtag
    11761 cctggctgcg cattcggcca aggggcagcc ggacgactgc ctgatgctgg cgcaggagtg
    11821 tagcgcggag ctaccggctc acgagctgcc ggttctgcaa agtgattgcg ccccgccgcc
    11881 acgcggtttt ccggaatgga acgcggtgca ggttggggtt catcgtccag cggtagcggc
    11941 gtttccagtt gctgttgtag cacgctcagc acgccctgat agataaaatc atgcaccaga
    12001 cgcgactgat ggaaacgcac ttcgtgtttg gcggggtgca cgttgacgtc cacctgatgc
    12061 ggatcgatct ccagatacag cacaaatgcc ggttgctgat cggcccccag tttgtcttcg
    12121 caggcctggc ggatcgcgtg attgatcagg cgatcgcgca tcatgcgacc gttcacgtag
    12181 caatactgaa tttctgccag tgcgggcgtg gtgtgatttg gatcggccac ccagccgcgt
    12241 agcgtgagat cgccgtgttg ccattcaatc gccagcgctt gttcaagaaa agcggtgccg
    12301 caaatcgcgc ctaagcgccg ttctttttgc ccgccttccg gcactgcgcg gtactgacgc
    12361 acaattttac cgttatgcga caggttgatc gtgacgtcga aacgcgccag cgcaatgcgg
    12421 cggatgatct catcaatgtg gttaaattcg gttttctcgg tgcgcaggaa tttgcgccgc
    12481 gccggggtgt tgtagaacag atccagcacc tccagcgtcg tccccacagg atgcgccgcc
    12541 ggttttaccg tcacgttcat atcgcgccct tcggcatagg cctgccaggc ttcctgctgt
    12601 tctgcggtgc gtgaagtgag cgtcaggcgg gaaaccgaac tgatactcgc cagcgcctca
    12661 ccgcgaaagc ccaggctgat aatggcttcg agatcgtcca gagaggcgat tttactggtg
    12721 gcatgacgag ccagcgccag cgccagctca tcttttttga taccgcagcc gttatcacga
    12781 atgcggataa gtttcgcccc accgcgttcg atatcaatat cgatacgcgt cgcacctgca
    12841 tcgaggctgt tttccaccag ttctttgact accgacgcag gtcgctcgac cacctcacct
    12901 gcggcaatct ggttcgccag ttgtggcggt aagacctgaa ttggcatcaa tcgtccttag
    12961 tttggcagcg tgcgatctgg cgtcgtcacc gtactggccg tttgtgccgt tgcaccctgc
    13021 ggcgcagatt gcatcggatg cgcaaggaaa taattgcgca ggcctttgta aatggcttct
    13081 gccagctgtt gttggtaatc gtcgctcgcc agcaaacgtt cttcgctgtt gttgctgata
    13141 aaaccggttt cgaccagtac tgacgggata tccggcgaac gcagaacgcc gagactcgcg
    13201 tgttctggtc ggcgtttatg tatttcgcca atgcgttgca actgactgat catactggtc
    13261 gctacatcat accctacccg ctgggaatga ccgaactgta aatccagcac cgcctggctt
    13321 aaatagggat cagactgact gttcgccagc acatcgcccg ccccacccag cagctccgaa
    13381 tgtttctcgt gctgctccag ccagctggcc atttcgctgt tggcgcgacg gttagagagc
    13441 acccatacgg aagcgccagt cgcactgcgg ttcggtgcgg catcagcgtg aatcgacacg
    13501 aggaaattag cgttttgctt acgtgccaca tcgctgcgcc ccatcaccga aataaagtaa
    13561 tccccgtcac gggttaaaac gcctttaaac atcggatcgt cattgagcaa agtacgcaat
    13621 ttacgcgcga tggcgatggt gacatttttc tcccgcgtac caccggggcc gatagcgcca
    13681 gggtcctgac cgccgtgtcc ggcatcaata gcgatgataa ttttatcgcc agtgttagcc
    13741 gtcgcgcgcg ctgccggacg cgttaccgta ttactgctga taacacccgt agtgcggtta
    13801 ctttccgttt taaacggatt gcgcgccggt tcgctgacgc gcggtgcgac aaccgcaggc
    13861 gtttcaacgc gtttcgcaac cacgggcggc ggaggaggcg gtggcggcac atcggcgtta
    13921 atcgtaaaga cgacagtgta attgctgcca ttctgccgct tcaccgcttc ggttttacca
    13981 ttttcggtaa gatcgaccac cagccgtagc gtttgtgcat ctttaggcgt tccagagcga
    14041 atcgccttca ccagattatt gccgctgaac aacaacggca gtccctgaat cacgcccgtt
    14101 tgtttgatat cgagcgccac ggtgcgtttg ctttgatggc taaacgcata atcaggatcg
    14161 ccaataaaac tcaacgttat ccgcgcctgt tgattaccgt tagaaacctg aatatcagag
    14221 agcgtcgcgg cacccaccgg cgtgcacagc agcagcagcg tcgctaccaa ccaatttctg
    14281 atgcgataca tcatcccgcc acctttcaaa ggttaaccgg ctaaacgcgc cagcaacaat
    14341 tcacccgcag aggaaaccgc actcacgcgc gcctcacggc cttgtgcctg ataatcaatg
    14401 tgtatttcga catccgggtc aggaagaaca cctgtacctt gttgtggcca ctccaccagg
    14461 cagatggcat cgttggcaaa ataatcgcgg atccccataa actccagctc ctcgggatcg
    14521 gcaaggcggt acaaatcaaa gtgataaacc attaaattgt cgagcgtata gggttcgacc
    14581 agcgtataag tggggctttt gacattaccc tgatgaccca gagcttgtaa aaagccccgg
    14641 ctaaaggtgg tcttacctgc gcctaaatcg ccatacagat agattacggt tgcgccatcg
    14701 caggctttcg ctacccgctc gcccaggtct aatgttgcct gctcatcagg gagcggaatt
    14761 actcgattca tcatggtttt tatcagtcac ttccgggtta acaatacgct gtagcgtgga
    14821 aaagagatcg gttgccagca tcccgcgcgt tccaaaacgc gccgccagta cgtcagctgc
    14881 cgcaccgtgc gcgacacagc ccgcacaggc ggcatcatac ggcgacagtt tttgcccaag
    14941 caatgcgcca ataataccag agagcacatc gcccatgccg ccgctcgcca tgcctgcatt
    15001 tccggcatca ataatgccta aagcgtcagg atgggcggcg accacggttc cggcaccttt
    15061 cagcaccgct acgccgccat aacgttgtac cagacgtttg gcgcaatgta agcggtcact
    15121 ttcaatttca gcgacggaac agcctaacaa ccgtgcggcc tcgccaggat gcggcgtgat
    15181 cacgcgattg tgacgcttat cgggattgat tgccagcagg ttcaatgcat cggcatccca
    15241 caacatcggt ttgcgaaaat tctcaacttt ttgcagtgct tttttccccc actcttgctg
    15301 gcccagaccg ggaccaatca ccaccacatc ggcccattcc aggctttcgg taagagagtc
    15361 catcgtcagt tcatgcacca tcaattccgg tcgtgcagtc agcagcggcg caatgttttc
    15421 actgcgggtc agtactcgga ctaaaccagc accagcacgc agcgccgctt cccccgtcat
    15481 acgaatagcc cccgccgtgc cgtgatcgcc accgataatt accagccgcc cgtgatcgcc
    15541 tttatgcgaa gtcgggcgac gcggttttag ccagtgagaa agttgttctg ctgaaaaccg
    15601 ctgaattttc gtctcctgac ctgccagcca actatccagc cccagtgagt caaaatgcag
    15661 ttgtccggta acatcccgcg cttttccagt gagcaagcct ggtttcagcg caataaaagt
    15721 gatggagtga tcggcgttga tcaccgcgcc tggcgtagcg ccagtttcag ccagcaggcc
    15781 ggaagggata tcaaccgcca caatcggcgc aggatgggaa ttagcgtggt cgattaactg
    15841 gctaatggat tcgcggggcg cttgccgcaa accggtaccg agcagcgcat caacaatcag
    15901 atctaccgat tcgggccaga caatattcga agcatggatc tcgccacccg cgtttaacca
    15961 tgcttcgcgt gccagcgcgg cctcttccgg caacggtttg tcgctctcct gggcaaacaa
    16021 cgtgacctca atgccgaccg ctttggccag tcgcgcgacc acgtagccat cgccgccgtt
    16081 attaccatga ccgcacagca ccagccagtg gcgggcgtca ggatacgccg aacgacacac
    16141 ctggaatgcg gcctcgccag cgcgaagcat cagctcatag agtgtgagcc ccagcacatc
    16201 tgccgcctcg cgttctccgc ggcggatatc gtcggcgtac cagacggtgt gtggtatact
    16261 tacggggttt ttcttcattg tatggtccgt catgtcagag cccctcgatc tcaatcagtt
    16321 agcgcaaaaa attaaacagt gggggctgga actgggcttt cagcaggtag gtattaccga
    16381 taccgatctc agcgagtccg agcccaaact gcaagcatgg ctggacaaac aataccacgg
    16441 cgaaatggac tggatggcac gtcacggtat gctgcgcgct cgccctcatg agttattgcc
    16501 cggtacgctg cgcgtgatca gcgtgcggat gaattacctt cctgctaacg ccgcatttgc
    16561 cagcacgctg aaaaacccca aactcggcta tgttagccgt tatgcgctgg gccgtgacta
    16621 tcacaaactt ctgcgcaacc gactcaaaaa gctgggcgag atgattcagc aacattgtgt
    16681 ttcgctgaat tttagaccgt ttgtcgattc tgcgcctatt ctcgagcgcc cgttagctga
    16741 aaaagctggg ctcggctgga caggtaagca ctcacttatc ctcaatcgcg aggccggttc
    16801 gttcttcttt ttaggcgaat tgctggtcga tattccgctg cccgtggatc aaccagtcga
    16861 ggaaggatgc ggtaaatgcg tggcctgtat gacgatttgc ccgaccggtg ccatcgtcga
    16921 gccatatacc gtcgatgctc gccgctgtat ctcttatctc accatcgaac ttgaaggggc
    16981 gatcccggaa gagttgcgac cgttaatggg aaaccgtatt tacggttgcg atgactgcca
    17041 gcctatctgc ccgtggaatc gctattcaca actcaccaca gaagaggatt tcagcccgcg
    17101 taagccgcta cacgcaccgg aactcattga gttattcgcc tggagcgaag agaagttttt
    17161 aaaagtcacg gaaggatcgg cgattcgtcg aattggtcac ctgcgttggc tgcgtaatat
    17221 cgccgtagca ttaggcaatg ccccttggga tgaaacgatt ttgacagcgc tggaaagtcg
    17281 taaaggtggg cacccacttc tcgatgagca catagcgtgg gcgattgcgc agcaaataga
    17341 gagacgaaat gcgtgcatag tcgaagtgca attgccgaaa aaacagcgtc tggttcgggt
    17401 gattgaaaaa gggttaccgc gtgacgcctg attcatccac agcttgtgaa taaaaataaa
    17461 aacgcattgc aattcaagac gaataaaatc gtcaagtgat cgactcgaca aaacggaatc
    17521 ttaattttta acttatctat caataggtta aaaaaatact attcaccgtg cgaagtttct
    17581 ttgaaattaa gattacagcc tgaggctgtg gataactctg tttacaagag tttgtcagag
    17641 acaacgaaaa ccagcaataa cgcatcgctg tggatatttt attgagagaa gaatttggag
    17701 cgggaaacga gactcgaact cgcgaccccg accttggcaa ggtcgtgctc taccaactga
    17761 gctattcccg cttgggtggt ctgtgcctta cagcactttt caaattttgg agcgggaaac
    17821 gagactcgaa ctcgcgaccc cgaccttggc aaggtcgtgc tctaccaact gagctattcc
    17881 cgcttgggtg gtctgtgctt tgcagcactt ttcaaatttt ggagcgggaa acgagactcg
    17941 aactcgcgac cccgaccttg gcaaggtcgt gctctaccaa ctgagctatt cccgcgtact
    18001 acttaatttt gctttcgtaa ttttcgcatt tctgcgtcgt tacgggaggc gcattatacg
    18061 agaaatcttt ccctctgcaa ccccccaaaa aagttttttt tgcgttttta tctcaagtgc
    18121 tgaaattatc agcaagacgt tcaatttagc gtcaaaatca cgccgccgca agtggattcc
    18181 ggcgcttacg tgaccaggaa aaattttaca gcttgataaa atgctcgcgg tagtaagcca
    18241 gctccgccac cgattcacgg atatcatcca tcgcctgatg cgtcccctgc ttggtaaaac
    18301 tatccagaat ttccggcttc cagcggcgcg ccagctcttt cagggtgctg acatcgagat
    18361 aacggtagtg gaagtaggct tccagctccg gcatgtattt aaacaggaaa cgacggtcct
    18421 gtccgatgct gttaccgcaa atcggcgatt ttcccgcagg cacccactgt tttaagaatt
    18481 cgagcgttgc cagttcagct tcccgatcgc ccatcgtgct cgctttcacg cgctctacca
    18541 gcccgctggc ggtatgggtg cgcacgttcc agtcatccat cagcgccagc tgttcatcgg
    18601 actgatgtac tgcaatggtc ggcccttctg ccagaatatt caggttggca tcggtcacca
    18661 gcgtggcaat ctcaataatg cgatcgcgct cgggatccag accagtcatc tcaagatcga
    18721 tccaaatcag gttgttttca ttggcactca tgctattttc cacccattca cagtgactat
    18781 attgatccac aattgcgtgt atcatagatg ttttgcccat caggggcgac caggagtctg
    18841 tacgattgag taaaaataaa ctctccaaag gccagcagcg ccgcgtgaac gccaatcacc
    18901 agcgtcgtct taaaacgtct aaggagaagc ccgactacga cgacaatctg tttggcgagc
    18961 ctgatgaagg tatcgtcatc agccgctttg gtatgcacgc tgatgtggaa tccgccgatg
    19021 gcgacgttca ccgctgcaat attcgccgta ccatccgttc gctggtgacc ggcgaccgtg
    19081 tagtctggcg tccgggtaaa ccggcggcgg aaggcgtaaa tgtcaaaggg atcgtggaag
    19141 cggtgcatga gcgtacctcg gtgttgacgc gtccggattt ctacgacggc gtaaaaccta
    19201 ttgccgccaa catcgaccag attgtcattg tctccgccat tttgccggag ctgtcactca
    19261 atattatcga ccgttacctg gtggcctgcg aaaccttgca gattgagccg attattgtgc
    19321 tcaacaagat agacctgctg gacgacgaag gcatggcgtt cgtcaacgag cagatggata
    19381 tctaccgcaa tatcggttat cgcgtattga tggtttccag ccatactcag gatgggctaa
    19441 aaccgctgga agaggcgttg accgggcgca tcagcatttt tgccgggcag tctggcgtcg
    19501 gtaaatccag cctgctgaat gcgttgctgg ggctgcaaaa agagatcctg accaacgatg
    19561 tctctgacaa ctcggggctc ggtcagcaca ccacgaccgc cgctcggctg tatcacttcc
    19621 cgcacggcgg tgatgtgatt gattcccctg gtgtgcgtga gttcggcctc tggcacctgg
    19681 agccggaaca aatcactcag ggctttgtcg aattccatga ctatttaggt ctgtgtaaat
    19741 atcgcgattg caaacacgat accgatccgg gctgcgctat ccgggaagcg gttgaggaag
    19801 ggaaaatcgc ggaaacccgt ttcgaaaact atcaccgtat tctggaaagc atggcgcagg
    19861 taaaaacgcg taaaaacttt tctgatacgg atgactgaca attaagctaa ccgtcgttag
    19921 aatcgtcccc tttttttcag gatccggcat gttggccgga tcaggaacga caaaacaatg
    19981 gcctggaggc taccttgtta aattcattta aactttcgct acagtacatt ctgccgaaac
    20041 tatggcttac tcgcctggcg ggttggggcg caagcaagcg ggcaggatgg ctgacaaaac
    20101 tggttatcga tctgttcgtt aaatactaca aggtcgacat gaaagaggcg caaaagccgg
    20161 acaccgccag ctaccgcacc tttaacgaat tctttgtccg tccgctgcgt gacgaagtac
    20221 gcccaatcga taccgatccg aatgtactgg tcatgcctgc cgatggcgtt atcagccagt
    20281 tgggtaaaat cgaagaagat aaaatcctgc aagccaaagg ccacaactac agcctcgaag
    20341 ccctgctggc aggcaactat ctgatggcgg acctgttccg caacggtacg tttgtgacca
    20401 cttacctctc cccgcgtgac taccatcgcg tacacatgcc gtgcaacggt attctgcgtg
    20461 agatgatcta cgtgccgggc gatctcttct ccgttaacca tctcaccgct cagaacgtgc
    20521 cgaatctgtt tgcccgtaac gaacgcgtga tttgcctttt cgataccgaa tttggcccaa
    20581 tggcgcagat tctggtcgga gcgacgattg ttggcagcat tgagacggtc tgggcgggca
    20641 ccattacgcc gccgcgcgaa ggtatcatca agcgctggac ctggcctgcc ggggaaaacg
    20701 acggttctgt ggcactgctg aaaggccagg aaatgggtcg ctttaaactc ggttccaccg
    20761 ttatcaacct gtttgcaccg ggtaaagtga atctggttga gcaactggaa agcctgtctg
    20821 tcacgaaaat tggccagccg ctggcagtat ctaccgaaac ctttgttacg ccagacgctg
    20881 aacccgcccc acttcctgct gaagagatcg aagcagaaca cgacgccagc ccattggttg
    20941 acgataaaaa agaccaggtc taatccatca aaggaaacgc tgacgtgcgc ctgattatca
    21001 cttttctgat ggcctggtgc ctcagttggg gggcgtacgc cgcgacggcc cccgatagca
    21061 aacaaatcac tcaggaactg gagcaggcaa aagcggcgaa acccgcacag ccggaagtcg
    21121 tagaggcgct ccagtctgcc ttaaatgcgc ttgaggaacg aaaaggttcc cttgagcgca
    21181 tcaaacaata tcagcaagtt atcgataatt atccgaaact ctccgctact ctgcgcgcac
    21241 aattaaacaa catgcgtgac gagccgcgca gcgtgtcgcc gggaatgtct accgacgcgc
    21301 tgaatcagga aattctccag gtcagcagcc agttgctgga taaaagccgt caggcccagc
    21361 aagagcagga gcgcacccgc gagattgccg attcgctgaa tcaactgccg caacagcaaa
    21421 ccgacgcccg ccgtcagtta aatgagatcg agcgccgcct gggaacgctt accggcaata
    21481 ctccgctcaa tcaggcacaa aatttcgcgt tgcagtctga ctctgcacgt cttaaggcgc
    21541 tcgttgatga actggagctg gcgcagctgt ctgccaataa ccgccaggaa ttagcgcgct
    21601 tacgctcaga gctggcggaa aaagagagcc agcaactgga tgcgtatttg caggccttgc
    21661 gtaatcaatt aaacagccaa cgtcagcttg aggcggagcg ggcgctggaa agtaccgaat
    21721 tgctggcaga aaacagcgcc gatttgccga aagatatcgt cgcgcaattc aaaattaacc
    21781 gcgaactatc ggcggctttg aatcaacagg cgcagcggat ggatctcgtt gcctcgcaac
    21841 agcgtcaggc tgccagccag acgttacagg tccggcaggc gttgaatacg ctgcgtgaac
    21901 agtcgcaatg gctgggatcg tccaatctgc tcggcgaagc gctgcgggcg caggtggcac
    21961 ggctgccaga aatgccaaaa ccacaacagc ttgataccga aatggcgcag ttgcgtgtgc
    22021 aacggttacg ttatgaggat ctgcttaata aacagccgct gctacggcaa atccatcagg
    22081 ccgacggtca gccgctgacc gccgagcaaa accgtattct ggaagcacaa ctgcgcactc
    22141 agcgtgagtt gctgaactca ttgttgcagg gtggcgacac gctactattg gaactgacca
    22201 agctgaaagt ctccaacggg caactggagg atgcgctgaa agaggtgaac gaagcaacgc
    22261 accgctatct gttctggacc tctgacgtgc gcccgatgac catcgcctgg ccgctggaaa
    22321 tcgcccagga tctgcgtcgt ctcatttcgc tggacacctt cagtcagttg ggcaaagcca
    22381 gtgtgatgat gctgaccagc aaagagacaa ttttgccgct gtttggcgcg ttgattctgg
    22441 tcggttgcag tatttactcg cgccgctatt tcacccgttt tcttgaacgt tcggcggcga
    22501 aagttggcaa agtgactcag gatcacttct ggctgacgtt gcgcactctt ttctggtcga
    22561 ttctcgtcgc gtcaccgtta ccggtgctgt ggatgacgct gggttacggc ttgcgcgagg
    22621 cgtggcctta tccgctggcg gtcgcgattg gcgatggtgt aacggctacc gtgccgctgc
    22681 tgtgggtagt gatgatttgc gccacctttg cccgcccgaa cggcttgttt atcgctcatt
    22741 ttggctggcc gcgcgaacgt gtttcccgtg ggatgcgcta ctacctgatg agcatcgggc
    22801 ttattgtgcc gctgattatg gcgctgatga tgttcgataa cctcgacgac cgtgaattct
    22861 ccggttcgct gggacggctt tgctttatcc tcatttgcgg tgcgctggcg gtggtcaccc
    22921 tcagcctgaa aaaggccggg atcccactgt atctcaacaa agagggcagc ggcgacaaca
    22981 ttaccaacca tatgctgtgg aacatgatga ttggcgcgcc gttggttgcc attctggcgt
    23041 cggctgtggg ttatctggca acggcacagg cgctgttagc gaggcttgaa acctcggttg
    23101 ccatctggtt cctgctactg gtggtttatc acgttatccg ccgctggatg ctgatccagc
    23161 gccgcaggct ggcgtttgac cgggcgaagc atcgccgggc agagatgtta gcgcaacgtg
    23221 cgcgtggcga agaggaagcg catcatcaca gtagcccgga aggagcaatt gaagtcgatg
    23281 aaagcgaagt cgatctcgat gccatcagtg cgcaatcctt gcggctggtg cgctcaattt
    23341 tgatgttgat cgccctgctt tctgtcattg tgctgtggtc agaaatccat tccgctttcg
    23401 gcttcctcga aaatatttcg ctgtgggatg tcacctccac ggtacaaggc gtagaaagtc
    23461 tggagccaat taccctcggt gcggtgctga ttgccattct ggtgtttatc atcaccacgc
    23521 agctggtgcg caacctgccc gcgctgctgg aactggcgat tttgcagcac ctggatttaa
    23581 cgccgggtac aggctacgcc atcaccacca tcaccaaata tctgctgatg ctgattggcg
    23641 ggctggtcgg cttctcgatg attggtattg aatggtcgaa attgcagtgg ctggtcgccg
    23701 cgctcggtgt tggtctcggt tttggtttgc aggaaatttt cgccaacttt atctctggtc
    23761 tgattatcct gttcgaaaaa ccgattcgca ttggcgatac ggtgacaatt cgcgatctca
    23821 ccggtagcgt gacgaaaatt aacacccgcg ccaccaccat cagcgactgg gaccgtaaag
    23881 agataatcgt gccgaacaag gcgtttatta ccgagcagtt tatcaactgg tcgctctctg
    23941 actcggttac gcgcgtagtg ttgacgattc cggcccctgc tgatgccaac agtgaagaag
    24001 tgacggaaat cctgctcacc gcagcgcgtc gctgctcgct ggtgatcgac aacccggcac
    24061 cggaagtctt cctggtggat ctgcaacaag ggattcagat tttcgagctg cgtatttatg
    24121 ccgctgagat gggtcaccgt atgccgctac gccatgagat ccaccagctg attctggctg
    24181 gcttccatgc ccacggtatc gatatgccat tcccgccctt ccagatgcgt ctggaaagcc
    24241 tcaacggtaa acaaacgggg agaacgctga cgtctgcggg caaaggtcgt caggcgggaa
    24301 gtttgtaatg catgaagctg ccaaatgatg tcaacgtggc tcatttagca gcttaacatc
    24361 atcttcagtt atcaccggcc ctggagagta tcatccatac gcattacttc ctgacaactc
    24421 atcgccggag agacgatgag ttgcaggatt tcatatgatc agtcgagagc taactcaggt
    24481 ttattcgcca tgcgactgca atagttctgg taaatcgcca tcgctaacag cgagaagaag
    24541 acagggccgc caatcatcca caatgtgctg tcccagtctc cggcttcaac cacaggcgga
    24601 ataatggtga agacgttcgc aaatgtcacc accagtacga caaccaccgt cgcaatcatt
    24661 gccgacatac gcgttttgaa aatcacaaat ggtctgtcga gatcctgacg tgctttaaag
    24721 aatgggaaag ccagcgcgag gaacaggtaa ggaag
//
LOCUS       DAJTMC010000048        24632 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.048, whole genome shotgun sequence.
ACCESSION   DAJTMC010000048 DAJTMC010000000
VERSION     DAJTMC010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24632
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.048"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            <1..421
                     /gene="speG"
                     /locus_tag="OO130_001721"
     CDS             <1..421
                     /gene="speG"
                     /locus_tag="OO130_001721"
                     /EC_number="2.3.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
                     /protein_id="HCR5365173.1"
                     /translation="TTSHIHDQSERRFVVECDGEKAGLVELVEINHVHRRAEFQIIIS
                     PEYQGKGLATRAAKLAMDYGFTVLNLYKLYLIVDKENEKAIHIYRKLGFTVEGELMHE
                     FFINGQYRNAIRMCIFQHQYLAEHKTPGQTLLKPTAQ"
     gene            complement(424..1134)
                     /locus_tag="OO130_001722"
     CDS             complement(424..1134)
                     /locus_tag="OO130_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HCR5365174.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            1242..1547
                     /locus_tag="OO130_001723"
     CDS             1242..1547
                     /locus_tag="OO130_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HCR5365175.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYTRTTSANVSAPAQSTQDGAPAEPQ"
     gene            1746..2312
                     /locus_tag="OO130_001724"
                     /pseudo
     CDS             1746..2312
                     /locus_tag="OO130_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416104.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            2294..2422
                     /locus_tag="OO130_001725"
                     /pseudo
     CDS             2294..2422
                     /locus_tag="OO130_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310323.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
     gene            2465..3079
                     /gene="dmsD"
                     /locus_tag="OO130_001726"
     CDS             2465..3079
                     /gene="dmsD"
                     /locus_tag="OO130_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HCR5365176.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQYEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELSRLTLAQWQSQLLIPVAVKPLFR"
     gene            3238..4530
                     /gene="clcB"
                     /locus_tag="OO130_001727"
     CDS             3238..4530
                     /gene="clcB"
                     /locus_tag="OO130_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HCR5365177.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTEMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(4483..5178)
                     /gene="bioD"
                     /locus_tag="OO130_001728"
     CDS             complement(4483..5178)
                     /gene="bioD"
                     /locus_tag="OO130_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCR5365178.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(5303..6523)
                     /gene="mlc"
                     /locus_tag="OO130_001729"
                     /gene_synonym="dgsA"
     CDS             complement(5303..6523)
                     /gene="mlc"
                     /locus_tag="OO130_001729"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HCR5365179.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNLGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPIISDSIRQQALPTYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLHG"
     gene            complement(6658..7551)
                     /locus_tag="OO130_001730"
     CDS             complement(6658..7551)
                     /locus_tag="OO130_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5365180.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPGVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPNVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRAQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPASRNFRIHLLNALR"
     gene            7658..8910
                     /locus_tag="OO130_001731"
                     /pseudo
     CDS             7658..8910
                     /locus_tag="OO130_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            9307..9621
                     /locus_tag="OO130_001732"
     CDS             9307..9621
                     /locus_tag="OO130_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid shock protein"
                     /protein_id="HCR5365181.1"
                     /translation="MKKQIEGMTMKKVLALVVAAAMGLSSAAFAAETATTTKAAPAKT
                     THHKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHSHQQPAKPAAQP
                     AA"
     gene            9714..9797
                     /locus_tag="OO130_001733"
     CDS             9714..9797
                     /locus_tag="OO130_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5365182.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            9897..10718
                     /locus_tag="OO130_001734"
     CDS             9897..10718
                     /locus_tag="OO130_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HCR5365183.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTAQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVVLRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTNDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(10757..11086)
                     /gene="mdtI"
                     /locus_tag="OO130_001735"
     CDS             complement(10757..11086)
                     /gene="mdtI"
                     /locus_tag="OO130_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HCR5365184.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(11073..11438)
                     /gene="mdtJ"
                     /locus_tag="OO130_001736"
     CDS             complement(11073..11438)
                     /gene="mdtJ"
                     /locus_tag="OO130_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HCR5365185.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            11850..12884
                     /gene="tqsA"
                     /locus_tag="OO130_001737"
     CDS             11850..12884
                     /gene="tqsA"
                     /locus_tag="OO130_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HCR5365186.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            complement(12909..14297)
                     /gene="pntB"
                     /locus_tag="OO130_001738"
     CDS             complement(12909..14297)
                     /gene="pntB"
                     /locus_tag="OO130_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HCR5365187.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQDVIVFKRSMNTGYAGVQTPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(14308..15840)
                     /gene="pntA"
                     /locus_tag="OO130_001739"
     CDS             complement(14308..15840)
                     /gene="pntA"
                     /locus_tag="OO130_001739"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HCR5365188.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     TGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRRVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            16364..17308
                     /locus_tag="OO130_001740"
     CDS             16364..17308
                     /locus_tag="OO130_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR5365189.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            17494..18876
                     /locus_tag="OO130_001741"
     CDS             17494..18876
                     /locus_tag="OO130_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5365190.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWFVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLVLGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAASLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            18913..19635
                     /gene="folM"
                     /locus_tag="OO130_001742"
     CDS             18913..19635
                     /gene="folM"
                     /locus_tag="OO130_001742"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HCR5365191.1"
                     /translation="MGKAQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLIKAGAQCIQADFSTNDGVMAFADEVLKSTHGLRAILHNASAWMAEKLGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHIAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(19632..19944)
                     /locus_tag="OO130_001743"
                     /pseudo
     CDS             complement(19632..19944)
                     /locus_tag="OO130_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
     gene            20070..20789
                     /gene="rstA"
                     /locus_tag="OO130_001744"
     CDS             20070..20789
                     /gene="rstA"
                     /locus_tag="OO130_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HCR5365192.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGITICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     MPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRNVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            20793..22094
                     /gene="rstB"
                     /locus_tag="OO130_001745"
     CDS             20793..22094
                     /gene="rstB"
                     /locus_tag="OO130_001745"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HCR5365193.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLYERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLVQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            22170..23099
                     /gene="tus"
                     /locus_tag="OO130_001746"
     CDS             22170..23099
                     /gene="tus"
                     /locus_tag="OO130_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HCR5365194.1"
                     /translation="MARYDLVDRLNTTFRQMEQELAAFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRSLT
                     VLHDPATLRFGWANKHIIKNLHCDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(23096..24499)
                     /gene="fumC"
                     /locus_tag="OO130_001747"
     CDS             complement(23096..24499)
                     /gene="fumC"
                     /locus_tag="OO130_001747"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HCR5365195.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
BASE COUNT         5946 a         6342 c         6021 g         6323 t
ORIGIN      
        1 gaccacttca catattcacg atcagagcga acgacgcttt gtggtggaat gtgacggcga
       61 aaaagccggt ctggtggagc tggtggaaat taaccatgta catcgccggg cagaatttca
      121 gataattatc tccccggagt atcaggggaa aggtctggca acccgtgccg ccaaattagc
      181 aatggactat ggcttcaccg ttctcaatct ctataagctg tatctgatcg tcgataaaga
      241 gaatgaaaaa gcgattcaca tttaccgcaa gcttggcttt acggttgaag gtgaattgat
      301 gcatgagttt tttattaatg gtcaatatcg taatgccatt cgcatgtgta tattccagca
      361 tcagtatctg gcagagcaca aaacaccggg tcagactctc ctgaagccga ccgcacaata
      421 gcattaataa taatcgatcg tatttttgat ggtgtaaacc cgttcgacgg cgggttttac
      481 tccttcatca acaatgatca gctgacagtc caccggattg gcgtgactgt catattcaca
      541 gctctgtttt acattaccaa ctcgttgatt attcagtaaa gtaaccgctg tgtaatctaa
      601 ttttttgatc gggtccgttg atggcgtggc gctgacagat aatgttttgt cgttactttt
      661 cgtggtttta cccagtggat aaccctgatc atcatagcga tattccatct gcatttgttt
      721 gctgctggct ttaatcacga agccattatc atcggtttcc caactcaccc cggcagaagg
      781 taattctgct agctggcatt ttccctgtaa acgtactctc ttctccagcg tctcggcatc
      841 acggtaataa ttggcgtcca gtaccagagc gaccacggta ttattttcca gatccagtaa
      901 ttcgagtgaa tcaaaacagc cttcttccga caaagtccca gaaacacgtt tcgtcacttc
      961 accttgctca tccattaatg tctgagtgaa atcttttacc ggaccacgca gcggatcaaa
     1021 atcgaattca ttagagaaac tggccatctc aggggtaaat gaaagtgtta cctctgtgcg
     1081 gtcacatcct gtgaggaata tcgcgagtaa gcatggtagt aatttgtatt tcacaacagt
     1141 caccagagag tagagatgat tctcaatcat agtagcaaat acagtacttt acacgttaaa
     1201 tgctatgctt aaagaagtta tcttcgcgta aggagcttat gatgaaactc tcaacctgct
     1261 gtgccgcgct tctgctcgcc ctcgcctcac ccgcggtact cgccgcgccg ggatcctgtg
     1321 agcgcataca gagcgatata tcacagcgca ttatcaataa tggcgtaccc gaaagcagct
     1381 tcacattaag tattgtaccc aatgaccagg ttgatcagcc tgattcccag gtcgtcgggc
     1441 attgtgctaa tgatacgcat aaaattctct acacccgcac caccagcgct aacgtctctg
     1501 ctcccgcgca gtctacccag gatggcgcgc ctgccgaacc gcagtaatac attatcgact
     1561 gaacgccgga tatgacacat ccggcgattt gaacatacaa cataatccca ccttattact
     1621 catacccttc tattgatgtg gattaataat tcttaaccca aaatgggtag accccctcta
     1681 ttgttagcgc gctaaatatt caatacataa acatttatat aacgataaag aacagggagt
     1741 gagttatgtc caaaaatgac cgaatggtgg gcatcagccg cagaacactc gttaaatcaa
     1801 ccgcgatagg ttctctggcg ctggctgcag gcggtttttc tttaccgttc accctgcgca
     1861 gtgcagcggc aacggtacaa caggccagcg aaaaagtgat ctggggtgcc tgttccgtca
     1921 actgtggtag ccgctgtgca cttcgtctac atgttaaaga taatgaagtg acctgggtgg
     1981 aaactgacaa taccggcagc gatgagtacg gcaaccatca ggtacgcgcc tgtttgcgcg
     2041 gtcgctccat ccgccggcgt attaatcatc ccgatcgctt gaattaccca atgaaacgcg
     2101 tgggtaaacg cggtgaaggc aaattcgaac ggattagctg ggatgaagcc ctggatacta
     2161 tcgccagtag cctgaaaaaa accgtcgaac aatatggcaa tgaggctgta tatattcagt
     2221 actcttcggg gattgttggc ggcaatatga cccgctcttc gccatcagcc tcggcggtca
     2281 aacgcctgat gaactgctac ggcggttccc tcacgttgcc ggacttattc tggggctaat
     2341 cctcatactt ggcggtgaga tgatcgggcg tgtgctcttt tatggcctgc acatgacagt
     2401 aggaatggct attgcaggtt aacaaattgg tgcggggcaa cccgcacatt tcaggatgtt
     2461 aggaatgacc catttttcac agcaagataa tttttctgtc gcggcgcgcg tgttgggcgc
     2521 gctgttttat tacgctccag agagtgcgga agccgcacct ctggttgcgg tactcaccag
     2581 tgacggctgg gaaactcagt ggcctctacc agaagcgtca ttagcgccgc tggtgacggc
     2641 ttttcagaca cagtgtgaag agactcacgc ccaggcctgg cagcgtttgt ttgtcggccc
     2701 gtgggcactg ccgtctccgc cgtgggggtc ggtctggctg gatcgcgaat ctgtgctgtt
     2761 tggcgattca acattggcac ttcgtcagtg gatgcgcgag aaaggcattc agtttgaaat
     2821 gaagcaatac gaaccggaag atcattttgg ttcactgctg ttgatggctg cgtggttggc
     2881 ggagaatggt cgccagacag aatgcgaaga attgctggca tggcaccttt ttccgtggtc
     2941 aacacgtttt cttgatgttt ttatcgaaaa agcagaacac cctttctacc gtgcactggg
     3001 tgaactgtct cgcttaacgc tggcgcaatg gcaatcacaa ctgttaattc ctgtcgcggt
     3061 taaaccgctg tttcgatagg atcaccgtaa tattgccgga tggtgacgat tacgcgcaat
     3121 ccggcaataa taggttacag tgtcacgttt ttttatctct taaagcacgc actgcttttg
     3181 cggctggcct cttttgccgc aaaatagtcg cccgtgtttc attgcccatt tctgctcatg
     3241 catcatctac acatctatcc ggatctgcgc actatgttcc gccgtctgct tatcgcaaca
     3301 gtcgtcggta ttctcgcggc ctttgccgtt gccgggtttc gtcatgcgat gctgctactg
     3361 gagtggttgt tcctcaataa tgactccggc agtctggtca atgcagcgac aaacctttcc
     3421 ccctggcgac ggttgctaac tccggcgctc ggcggactgg cggcgggttt gttgctgatg
     3481 ggctggcaga aatttaccca acaacgccct catgcgccga ccgattacat ggaagcgttg
     3541 caaaccgatg gacagttcga ttacgcagca agcctggtta aatcgcttgc ctcgctgctg
     3601 gtagtaacca gcggcagtgc aattggtcgc gaaggtgcga tgattctttt agctgccctt
     3661 gccgcctcct gttttgccca acgttttacg ccacgccagg agtggaaatt atggatcgcc
     3721 tgtggcgccg cggcgggaat ggctgcggcc tatcgtgccc cgcttgctgg cagtttattt
     3781 atagccgaag tgctgtttgg cactatgatg ttggcctctc tcggcccggt gattatttcc
     3841 gccgtcgtgg cattgctggt tagcaatctg attaatcata gcgacgcgtt actctacaac
     3901 gtacaactct cagtgacggt tcaggctcgt gactatgcgc tgattatcag tacaggtgtg
     3961 ctggcaggtc tgtgcggacc actgttgtta acgttaatga acgcctgtca tcgtggattt
     4021 gtgagtctca aacttgcgcc gccctggcaa ctggcactag gcgggttgat cgtgggtctg
     4081 ctttccctgt tcacacctgc agtgtggggc aacggctata gcaccgtaca atccttttta
     4141 accgccccac cactgttaat gatcattgcc gggatcttcc tttgtaaact gtgtgccgtg
     4201 ctggcgagta gcggttccgg cgcacccggt ggtgtcttta caccgacgct atttatcggt
     4261 cttgccattg gcatgttgta tggtcgtagc ctgggattat ggttccctga tggcgaagaa
     4321 attacgcttt tactcggatt gaccgagatg gcgacactgt tggcggcaac cacgcacgcg
     4381 ccgattatgt cgacgttgat gatatgtgaa atgaccgggg agtatcagct actccccggt
     4441 ttattgattg cctgcgtcat tgcgtcggta atttcgcgga cgttacaccg tgactctatc
     4501 taccgccagc acactgcgca gcatagctaa gcggatgtat tgccccagtt ctcgctgttc
     4561 ggcgcgcggc agataaggca gttcaccaat gagcggtgcc ggaagttttt tacccagcac
     4621 atcaatgatt tccgcataat gcgccagtcc tgggttgatt cggttagcca cccagccaat
     4681 gagcggcagc ccgtcgttgg caatcgcctg agctgttagc agtgcatggt taatgcaacc
     4741 ttcctgaata ccgacaacca tcaacaccgg caactgttcc tgcaccaccc attcagagag
     4801 tggacgcaaa tcattcatca gactgcgcca gccgccagtc ccttctacca cgacatgatc
     4861 gactttatcg gtcaggtttg ccaggccgtt tgaaatgagg gtgtaattga ttgggcaact
     4921 gtgcgccacg ctactttctt cttcgcttaa cgcgatagga ttaactgctt cataaggtag
     4981 ttcgatggtt gaaacactct gcaacaccag ggcatcttta ttacgcagcc cttcgggtgt
     5041 ctctttgctc cccttcgcta cgggtttata tcccgcaact gtttttccct gggaggctaa
     5101 cgcttgtagc aatgcgcggg aaaccaccgt tttccctaca gaagtgtctg taccggtaat
     5161 aaagaaacgc ttcagcatca ctaactccac cgttatgctt cacaaatata aactaggaaa
     5221 ataattaacc ttgaaagtct aagttatgct ttcctggccc aaattgagat agcgcaaatt
     5281 ttggtagaac agttaaaaaa tgttaaccat gcaacagacg aatcaacaaa gaaccgttat
     5341 acatcgcgtc ttttaccagc gcagcgcctg ccatcgtgcc ctggttagaa aactgagtac
     5401 tctcaacgct aatgtgctga ctatacgtag gaagggcctg ctgacgtatg ctgtccgaga
     5461 tgatagggaa gaggatatct gccgctttac ttaacggtga gccaatcagt attttttgtg
     5521 ggttaaataa attcaccatg atggcaagaa tgcgcccgac atgcgcgccc accccggtaa
     5581 tgatgtcttt tgccagtaga tcgccgcgca atgccgcctg acacaatgag tccacggtta
     5641 acggttgccc atgtaacatc gagctcatgg attgattgag acgcagctgt gccagctcaa
     5701 gaatactgtc cacactggcg atggtttcga ggcagccgag gttcccgcaa taacagcgtt
     5761 taccatatgg gtcgacctgt gtgtggccta tttccacgag actactgctg cctgcgtgta
     5821 gcagatgacc atcggtaatg acgcccgccc ccacgttgtg atcgataacc acctgaatca
     5881 catcgcgcgc cccgcgtgag gcaccaaaca aggcctctgc catcgtccat gcgctgatat
     5941 catgctgaat ataaaccggt acgccggtat gctgctccag cgcctcgccg agcggcatct
     6001 cttttacatc ctcgtagaac ggcatgcgat gtacaatacc attttccgta tcaataattc
     6061 ccggcagggt tatggcaatc gaagttagac gctcaagttt tttctggtgg cggataaaaa
     6121 actgatcgat atgggaaatg atacgatcca gcaatggcaa gtcatctttt aacgccagtt
     6181 cctgcgactc ttccaccacc agtttgctgc tcagatcgcg cagagcaagg aaaatctcac
     6241 cgcgactaat gcgcagagaa agatagtgcc aggcttcagt ttcaaccacc agtcccaccg
     6301 ccggacggcc acggttcccc gcttctttga tttccagctc ttgcaccaag tgtgcttcga
     6361 gcatctcacg gacaatttta gtgatactgg caggagccag ctgcgccaga cgggaaagat
     6421 cgatacgcga gactggacca agctgatcaa tcaggcgata aaccgcgccc gcgttggtct
     6481 gctttatttg atcaatgtgc ccaggctggt tttcagcaac caccgcatac tccctatatt
     6541 ttcgcgctcc gaaataatct gtaggctatg gtgaagcact tcaatacgtg tcgtcaaatt
     6601 tttacttaga catgtgatta acagcacatt tttcgggctt tttcgctgaa atttccctca
     6661 cctgagagca ttcagcagat gaatacgaaa gttacgcgaa gctggacttt gttcatgatg
     6721 tttcggccag accaaccaca tttcagaaac cgcatcctct tcagcaatcg gcacccagcg
     6781 catttcgttg agctgagcac gtttaaatga agcaggcaaa attgaaacac ccagaccggc
     6841 ggaaaccaga ccgatgatgg tcattgcctc gcccacctcc tgagtgatga cgggcgtcaa
     6901 atggtaacgt cgcatcagcc cgagaatatc gtcatacagc cctgtcccga cgtgcggatc
     6961 aaaaaagacg aagggttctt tcgccagttc agccaacgtt acattcgggt tatttgccag
     7021 gggatgatcg tgcgggatca tcgccataag cggttcatgg acgatgactg cgtgttcaag
     7081 cgtctccggt aacgctgtgt tacgcagcaa tcccatatcc agcgttcctt caatgagcgg
     7141 agcgatttgc tcacgagtgt tcatttcgcg ggtttgtaaa tggacaccgg gataatcacg
     7201 gcgaaacagc gataacgtat cggacaccgc ccgaataaaa ggagcggacg aagtaaaacc
     7261 aatgcgcaac tcccccgctt caccctgatg cagtctttca gcgcgagcgg cagcgtcatc
     7321 taccatagac aggatttgcc gactatctgc aagaaactgt tttcctgctg ccgtcagcaa
     7381 tacactgcga ttagttcgtg ccagcagtcg ggcaccaatt tgttgctcca gtgcctgaat
     7441 ctgctgactt agcggtggtt gcgaaatatt caggcgggca gcggcgcgcc cgaaatgcag
     7501 ctcttccgca acagcaacaa agtaacgcag atgacgaagt tcaatattca tatttaaaac
     7561 atcttatttg agattattaa tatattagac agaacaattc gattttccta acctatgtat
     7621 aagcctgatc cacaggcata tttagcaagg atttcaagtg agccgtacta caactgttga
     7681 tggcgctccg gcaagcgaca ctgacaagca aagcatttct cagccaaatc aatttattaa
     7741 acgcggtacg ccgcaattta tgcgcgtcac cctggcgctg ttctctgccg gactggcaac
     7801 ctttgcactc ctctattgtg tgcagcctat ccttccggtg ctttcgcagg agtttggctt
     7861 aacccccgcg aacagtagta tttcactgtc catttccacg gcgatgttgg ctattggttt
     7921 gctgtttact ggccccctat ccgatgccat tggtcgcaaa ccagtgatgg tcacggcgct
     7981 actgttggcc tcaatttgta cgttatttcg acaatgatga ccagctggca cggcattttg
     8041 attatgcgcg ccttgattgg gctttcgtta agtggcgtgg cagctgttgg catgacttat
     8101 cttagcgagg aaatccaccc cagtttcgtg gccttttcga tggggttgta tatcagcggc
     8161 aactcaattg gcggcatgag cggacgctta attagtggtg tcttcacgga ctttttcaac
     8221 tggcgaattg ctctggcggc aatcggttgt ttcgcgctag cctcggcgtt gatgttctgg
     8281 aaaatccttc cggaatcacg ccattttcgc ccgacttcgc tgcgacctaa gacgttgttt
     8341 atcaactttc gtctgcactg gcgtgaccgg ggattaccgt tattgttcgc agaaggcttt
     8401 ttgctgatgg ggtcgttcgt cacgctgttt aattacatcg gctatcggtt gatgctttcc
     8461 ccctggcatg tcagtcaggc cgtggttggc ttattatcgc tggcttattt gaccggtaca
     8521 cggagctcac ccaaagccgg aaccatgacc acccgctacg ggcgcggtcc ggtgatgttg
     8581 ttttcgacgg gggttatgct gtttggttta ctgatgacct tattcagctc gctgtggctg
     8641 atctttgccg gaatgttact cttctcagca ggattcttcg cagcccactc agttgccagc
     8701 agctggatcg gcccccgcgc aaaacgcgct aaaggccagg cctcctcgct gtatctgttc
     8761 agttactatc tgggatcgag tattgccggg acgctgggtg atgttttctg gcataactat
     8821 ggctggaacg gcgtcggcgc atttattgct ctgatgctgg tcattgctct gctggtcggg
     8881 acgcgtttgc atcgtcgtct gcacgcctga aaaataagcc cggactgcgg taactacacg
     8941 tccggactta ttgcttgctc aaaccaacgt taatagccat cctaaaatag acgaagcgcc
     9001 agccaattcc cgcagtgcgt ctagcgtcat caggattata agtacccaaa taaacggatt
     9061 cattttgctg tgtgtcattt attactgatg cgcagttatt ctaccgcttt gtaagtagta
     9121 aaatagttaa cccgatcaag actactatta ttggtagcta aatttccctt aagtcacaat
     9181 acgttattat caatgctgta atttattcag cgtttgtaca tatcgttaca cgctgaaacc
     9241 aaccactcac ggaagtctgc cattcccagg gatatagtta tttcaacggc cccgcagtgg
     9301 ggttaaatga aaaaacaaat tgagggtatg acaatgaaaa aagtattagc tctggttgtt
     9361 gccgctgcta tgggtctgtc ttctgccgcc tttgctgcag agactgcgac gaccaccaaa
     9421 gcagcgccgg cgaaaacgac acatcataaa aaacagcata aagcagcacc tgcccagaaa
     9481 gcgcaggcag ctaaaaagca tcataaaaat acgaaagctg aacagaaagc ccctgaacaa
     9541 aaagcgcagg cagcgaagaa acacgccggg aaacacagtc atcagcaacc ggcaaaacct
     9601 gctgcacaac ccgcagcgta agttttcaac agtaatgctg gcgcgccccc tcgcgcctga
     9661 aaattacggt gctaagcggg taacgtttag caccgccttt agcggagggt agtatggtgg
     9721 gccgttatcg ctttgagttc attcttatca tccttatttt atgcgcactg attgccgccc
     9781 gtttttatct ttcctgactg tagttatctg attttactcc cactttcatc ccgtcccgtc
     9841 tatagtattt atgagggttt gcttttaata atcataatta cccaccagag tgtgatatgc
     9901 gtacaaccat tgctgtagtg ttgggtgcaa ttagtttgac gtctgctttt gtgtttgcag
     9961 ataaaccaga cgttgccaaa tcggcaaacg atgaggtcag caccctgttt tttggtcatg
    10021 atgatcgtgt gccagtgaat gacacggccc aatcaccgtg ggatgcggtt gggcaactgg
    10081 aaacggccag cggcaattta tgtacggcga cgctgattgc acccaatctg gcattaacgg
    10141 caggacactg tttattgaca cctccaaagg gtaaagccga taaagcagtg gtgctgcgtt
    10201 ttgtgtcaaa taaaggtctt tggcgctatg agatccacga catagaaggc cgcgttgatc
    10261 cgacactggg aaagcggtta aaagcagatg gggatggttg gattgtacct cccgcagccg
    10321 cgccgtggga cttcggattg attgtgctac gtaatccccc ttctggcatt acaccgttgc
    10381 cgttatttga gggagataaa gccgcgctta ctgccgcatt aaaatcggct ggccgtaaag
    10441 taactcaggc gggataccct gaagatcatc tcgatacgtt gtacagtcat caaaactgtg
    10501 aagtgactgg ctgggcgcaa acgtcggtga tgtcacatca gtgcgatacc ttgccgggtg
    10561 acagcggttc gcctctgatg ttgcatacca atgacggctg gcaattaatt ggggtgcaaa
    10621 gttcggctcc tgccgcgaaa gatcgctggc gcgccgataa ccgggccatt tctgttaccg
    10681 gttttcgcga caagctggat caactgtcgc aaaaataatg ttcaaacgct gcccgactgt
    10741 gcgggcagcg tgttcatcag gcaagtttca ccatgatcat tccagccaac agcaagacca
    10801 ggccaatcca gcctttacga tttaaccgtt gaccaaacaa gatccaacct gcggctaacg
    10861 tggcggcaat accaaacccg ccccacaatg cataagcgac agacaagtcg atccctttaa
    10921 cggcctgaga aagcgcacta aaggcagcca gcaccgccgc cagggagagc aagccaaata
    10981 ttttgcgacg aaagccgtca gaaaatttca aaaagacgtt agcaacgatt tccagcacga
    11041 ttgccaatgc cagccaggcg gcgtgaaccc attcaaactg cgccatggtt tacctccagt
    11101 tcaggtttac gcgctttacg ggtacctgat tttatcaaca caatcccggc gaccagggtg
    11161 gttaacccgg caattttcat cagcgataaa ctttcgtcga ataacaaaac gctaaacaag
    11221 gtaataaata aaataccgat accttcccac aacgcataag ctacgcctaa ggcgatcttt
    11281 ttaacggcga aagagagaaa tatatacgac agagaaatca tcaccagcat taaaataaag
    11341 ccgccatttc cctcactgac gctcgcccat ttcattgaca gcgtaccggt aatttctgta
    11401 gcaatagcca ggcctaataa aatccaataa atatacattg tccttctcct gcaagagaat
    11461 tattttaatt ttcgcttaat tcagcgaaat caaagtaaag tggtcaagcc ccgcgcatag
    11521 ggcaagctta ggcagaagaa aggactaaag cgcgttgcgc cagtgctgct cacctacgag
    11581 caagatagtg gatgaggtac aaagatgggt aaatgttgaa aataaggtcc tgaacaaatt
    11641 gtccataata ttacaattat ccgcagtgtt gcttctcgtc atcgcggatg ataattgtcc
    11701 tcggtagttg aacacgcctg atttgtatca tagcttaaga attaactcaa aatattttca
    11761 cttctttacc tgagcggttt gattttcgtt atgatgacgg agcgaaaaag acattattat
    11821 tagcaaagga agaaaaaacg gggacaagca tggcaaagcc gatcatcacg ctcaatggcc
    11881 taaaaatcgt cattatgttg ggaatgctgg tcattattct ctgcggtatc cgttttgccg
    11941 ccgagatcat cgtgccgttt attctcgcat tatttattgc tgttattctt aacccgctgg
    12001 tgcaacacat ggtccgctgg cgagtgccgc gtgtactggc ggtgtcgatt ttgatgacca
    12061 tcatcgtgat ggcgatggtg ttgctattag cttatctggg ttccgcgctc aacgagttga
    12121 cgcggacgtt accgcaatat cgcaactcta ttatgacgcc gctgcaagct cttgagccgt
    12181 tgttgcaacg cgtagggatt gacgtctcag ttgaccagct ggcgcattac attgatccga
    12241 acgcagcgat gacgttgctc accaacttat tgacgcagtt atctaatgcc atgtcatcaa
    12301 tatttttatt gctgctgacg gtgctgttta tgctgctcga agtgccacaa ttgcccggaa
    12361 aatttcagca aatgatggcg cgtccggttg aagggatggc ggcgattcaa cgtgcaattg
    12421 acagtgtttc tcattatctg gtgctgaaaa cagccatcag catcatcacc ggcctggtcg
    12481 cctgggcgat gctcgccgca ctcgatgttc gcttcgcttt tgtctgggga ttgctggcct
    12541 ttgcgcttaa ttacatccct aatattggtt cagttctcgc ggcaatcccc cctatcgctc
    12601 aggtactggt gtttaatggc ttctacgaag cgttgctggt gctggcggga tatctgctga
    12661 ttaatctggt cttcggcaat attctggagc cgcgcatcat gggacgtggg ctggggcttt
    12721 ccacattggt ggtatttttg tcgttgattt tttggggatg gttgttagga ccggtgggta
    12781 tgctgctttc cgtgccgtta acaattattg tcaaaattgc gcttgaacaa acagcgggag
    12841 gtcaaagcat cgccgttctg ttaagcgatc tcaataaaga gtgacggcct ccgcagaggc
    12901 cgtcagggtt acagagcttt caggattgca tccacgctgg ctttggcgtc accaaacagc
    12961 atgtgggtgt tttccttgaa gaacagcggg gtttgcacac cagcatagcc agtgttcatc
    13021 gaacgtttaa agacaatcac gtcctgcgct ttccacactt ccagcacagg cataccagca
    13081 ataggactct tcggatcatc ctgcgccgcc gggttaaccg tatcgttagc accaatcacc
    13141 agtacggtat cggtatcagc aaagtcatcg ttgatctcgt ccatttccag cacgatgtca
    13201 tacggtactt ttgcttcagc cagcaatacg ttcatatgtc caggcaaacg ccccgcaacc
    13261 gggtggatac cgaaacgcac gttgatacca cgagcgcgca gtttctcggt aatttcagcg
    13321 acaggatatt gcgcctgcgc gactgccatg ccgtaccccg gagtaatgat cactgaatgg
    13381 gagtttttca gcagttccgc tgtctcttct gcggtgattt cgcggtgctc gcccacttcc
    13441 tgatcatcgc cagtagaaga gccgtcggtg ccgaaaccac ccgcaataac gctgataaag
    13501 gaacggttca tcgccttaca cataatgtaa gaaaggatag cccccgaaga accgaccagc
    13561 gcaccggtca caatcagcag gtcgttgctg agcataaagc ccgcagccgc agccgcccag
    13621 ccggagtacg agttcagcat cgacaccacc actggcatat ctgcaccacc gatggaggcg
    13681 actaaatgcc agccgaatac cagcgcaatg gcggtcatta ttagcaatgc cagcacttgc
    13741 aggccgacgc tgtccgtgcg aacaaataca atcagcagca ggaaggaaac gaccagagcc
    13801 gccaggttca ttttgtgacg gtttggcagc atcaacggtt tagacgaaat cttgccacac
    13861 agtttgccga acgccaccac cgaacccgtg aacgttaccg ccccgatgaa gataccgagg
    13921 aacacttccg tcaggtgaat attgaccaga atcggtgcca ttcccgcgtc atgatgcaga
    13981 tagctgttaa agccaaccag cactgccgcc agacccacga agctgtgcag gatcgccacc
    14041 agttctggca tttcggtcat ttcaactttc ttcgccagac ggataccaat tgccccacca
    14101 atgaccatcg ccagcaagat ccagccaaca ttacccgtat ccggtccaaa aatggttgcg
    14161 attaacgcaa tcgccatccc tgcgataccg aagttgttac cctggcgaga cgtttcatgt
    14221 ttcgaaagac cggccagact gaagataaac aggatcgcgg caacaatgta tgcagctgta
    14281 actaatcctc cagacatatg ttacccctta atttttgcgg aacattttca gcatgcgctg
    14341 agtcacggtg aagccaccga aaatattaat gctggctata agcaccgcga taaaactaag
    14401 gaagctaacc cagccgccct ggccaatctg caacagtgct ccgacaacaa taatccctga
    14461 aatcgcgttg gtgaccgaca tcaacggtgt atgcagcgcg tgcgatacat tccacaccac
    14521 gtaataaccg acaacgcagg ccagcgcgaa aacggtgaag tgcccaagaa actctttcgg
    14581 cgcaacgctt gccagccaac cgaaaaggat gattgccagc gccatcaacg cgtatttacg
    14641 ccacggtgag caggcacatt tttcctcagt tttcacttcc ggtgccgctt tttgtgccgc
    14701 ctgcggctga gctgatacct gaatcggcgg tgccggccag gtaatttcgc ccgcacggat
    14761 cacggtcacg cggcgaatca ccacatcatc aaaatcaaca gtgatattgc cgtctttctc
    14821 tttgcacaac agtttcagca gattaacgag gttagtaccg taaagctgcg aggattgcgt
    14881 cggcagacgg cccggaagat cggtataacc aatcactttg acaccatttt ccgtagtgaa
    14941 gatttcaccc ggcacggtgt attcacagtt gccaccgttt tgggctgcca ggtcgacaat
    15001 cacactgccc gtcttcatgg agtcaaccat ttcacgggta attagcttcg gcgctggttt
    15061 gcctggaata agcgcggtgg tgacaatgat atcgacctct tttgcctggg cggcaaagag
    15121 ttccatttct gctttaatga acgcgtccga catcactttg gcatagccat cgccgctgcc
    15181 cgcttcctct ttaaaatcca gctcgaggaa ttccgcgccc atactttgaa cttgttcttt
    15241 cacttccgga cgggtgtcga atgcacgcac aatcgcgccg agactgtttg ctgcgccaat
    15301 ggcggccaga cctgcaacac ccgcaccaat caccatcact tttgccggtg gcactttccc
    15361 ggccgcagta atttgcccgg taaagaagcg cccaaattca tgtgccgctt caacaatggc
    15421 gcgataaccg gcgatgttcg ccatcgagct tagtgcgtcc agcgattgtg cgcgtgagat
    15481 acgcggcaca gaatccatcg ccatcacggt cacgttacgt tccgcaagtt tttgcattaa
    15541 ttccggattc tgcgcaggcc agataaaact caccagcgtt gtcccaggat tcagtaacgc
    15601 aatttcatca tctaacggcg cattgacctt cagaatgatc tctgactgcc agacgctatt
    15661 cccttctaca atttcagcgc ccgcttgcac aaacgcttta tcgtcaaaac ttgccagttg
    15721 acccgcgccg ctctctaccg cgacggtaaa acccagtttc agcagctgtt ccactgtttt
    15781 tggcgttgct gcaacacggg tttcattggt taaccgttct cttggtatgc caattcgcat
    15841 gatattccct tccatcggtt ttattgatga tggtttgcct gtgtcaggag ccacacaagc
    15901 cgctcatgta cgagctaaat gttactccgt taaaataaat tagtaacaaa cgccctataa
    15961 cgtactgaaa attatgcctg tgatctagcg ccaaaaatca gtatttcggc gtgaactcgc
    16021 aaaatattaa cgattcagcc gtgatagtgg gataaacacc ttagaacgcc ggataaagac
    16081 tgataattgt cttcgacggt cgggtaaaac gagacatcgc cccggcacga ttcactactt
    16141 aacattaaat taacttatac aattcagttg cttcagtagt aatgatgctg atacggctgt
    16201 tttttaagca tagacggtca tttgagcagg attaaaattg gcttaaggaa tgtgatatga
    16261 aaaatgacgc agacagttac accgtttaaa tgcaataatc agccacgttt ctcgttaata
    16321 acaataccag tacctggttt gcgcaaggcg aaggattatt tttatgaagc ttaagaacac
    16381 cctcctggcg tcggcactgc tttctgctac ggcattctcc gttaacgcag caacagaact
    16441 gacaccggag caagcggcag cggttaaacc ttttgaccgt gtagtggtta ccggtcgttt
    16501 taatgctatt ggcgaagcgg tgaaagccgt ttctcgtcgc gcagataaag aaggtgccgc
    16561 ctctttttat gttgtcgaca cttctgattt tggtaacagc ggtaactggc gtgtggtcgc
    16621 tgacctctat aaagccgatg ctgaaaaagc agaagaaaca agtaatcgcg taattaacgg
    16681 tgttgtcgaa ctgccgaaag atcaggctgt tctgattgaa ccgtttgaca cggtcaccgt
    16741 ccagggcttc tatcgtagcc agccagaagt caatgatgcc atcaccaaag cggcaaaagc
    16801 gaaaggtgcc tactctttct acatcgttcg tcaaatcgat gccaaccagg gcggcaacca
    16861 gcgtattact gcattcatct ataaaaaaga tgctaagaaa cgtatcgtcc agagcccgga
    16921 tgtgatcccg gcagattccg aagcaggacg tgcagctctg gctgccggtg gcgaagccgc
    16981 gaagaaagtt gagatcccag gtgttgcgac taccgcatca ccaagttctg aagtcggtcg
    17041 tttctttgaa acccagtcat caaaaggcgg gcgttacacc gtcacgctcc cggatggcac
    17101 taaagtcgaa gaactgaaca aagcgaccgc agcgatgatg gtcccgttcg atagcatcaa
    17161 attctctggc aactacggca acatgaccga agtctcctat caggttgcga aacgtgccgc
    17221 gaagaaaggt gctaagtatt accacatcac ccgccagtgg caggaacgtg gtaataacct
    17281 gaccgtcagc gcagatctgt ataaataact attcagttac tcctataagg cggcttgatg
    17341 ccgcctttcg catttttagt cacaaatttc cccgcgacca ttgcatgcag acacatctct
    17401 tcgtaaaatc ccgcgcttca gtggcaaact ccatttttat gcgttttgcc ctaatattta
    17461 ttcttcttat cacgttttaa tcactggata tcgatggaaa agaaactggg actgagcgca
    17521 ctcaccgcgc tggtattaag ctcaatgctg ggcgcgggtg ttttcagtct gccgcaaaat
    17581 atggcggcag ttgccagccc ggcagcactg ctcatcggct ggggtattac tggcgctggc
    17641 attttattgc tggcctttgc catgctgatc ctcacgcgca ttcgtcccga acttgacggc
    17701 ggtatcttta cctatgcccg cgaagggttt ggcgagctaa tcggcttttg ttccgcatgg
    17761 ggatactggc tgtgcgcagt catcgccaac gtctcctatc tggtgatcgt tttttccgcg
    17821 ttaagctttt ttacggacac gccggaattg cgcctgttcg gcgatggtaa tacctggcaa
    17881 tcgatcgtcg gtgcatcggc tttattgtgg tttgttcact ttttgatcct gcgcggggtg
    17941 caaaccgctg ccagcattaa cctggtggcg acactggcaa aattgttgcc gctgggtctg
    18001 tttgttgtgc tggcgatgat gatgttcaaa ctggatacct tcaagctcga cttcaccgga
    18061 cttgtacttg gcgtacccgt ttgggaacag gtgaaaaaca ccatgctgat caccctgtgg
    18121 gttttcattg gtgtggaagg tgccgtggtt gtttctgcgc gtgcgcgtaa taaacgtgat
    18181 gttggcaaag cgacactgct ggcggttctc tccgctctgg gcgtttactt gctggtaacg
    18241 ctgctttcgc tgggtgtggt ggcacgtcct gaactggctg aaattcgtaa cccgtcaatg
    18301 gccggtctga tggtggaaat gatgggacca tggggcgaaa tcatcatcgc tgccagtttg
    18361 atcgtttccg tttgcggcgc gtacttaagc tggaccatca tggcggcaga agttccgttc
    18421 ctggctgcca ctcataaagc attcccacgc atttttgcgc gccagaacgc acaagctgcg
    18481 ccatccgcct cactgtggtt gaccaacatc tgcgtgcaaa tttgtctggt actcatctgg
    18541 ctgaccggtt ccgattacaa cacgttgctg accatcgcct cagaaatgat tctggtgccc
    18601 tatttcctgg tcggcgcgtt cttgctgaaa atcgcaaccc gtccactgca taaagcggta
    18661 ggtgtcggtg cctgcattta tggcttatgg ttattgtacg cctctggtcc aatgcacttg
    18721 ctgttatccg ttgttctgta tgcgccgggg ctgctggttt tcctgtatgc gcgcaaaacg
    18781 catacacatg ataatgtact gaaccgtcag gaaatggtgc tgattggcat gctgttaatt
    18841 gcctcagttc cggcgacctg gatgctggtg ggataacgtt catcccatcg ttggatagca
    18901 aaggagataa cgatgggtaa agcccagccc ttgccaatat taattactgg cggaggtcgt
    18961 cgcatcggcc tcgccctcgc ctggcatttc attaatcaaa agcaaccggt gattgtcagc
    19021 tatcggacgc actatccagc cattgatgga ctgattaagg caggtgcgca gtgtattcag
    19081 gctgattttt cgaccaacga cggtgtgatg gcgtttgccg atgaagtact aaaaagcacc
    19141 catggtctgc gtgctatttt gcataacgcc agtgcgtgga tggcggaaaa actgggtgcg
    19201 ccactggccg acgtactggc ttgcatgatg cagatccacg ttaatacccc atacctgctc
    19261 aaccatgcgc tggaaagatt actgcgtggg cacggacacg ccgccagcga tatcattcac
    19321 tttaccgatt atgtggtgga gcgcggtagc gacaagcata ttgcgtatgc tgcaagcaaa
    19381 gcggcactgg ataatatgac ccgctcgttt gcccgcaagc tggcaccgga agtgaaagtg
    19441 aattctattg ctccatcgct gatcctgttt aatgaacatg atgatgccga atatcgacaa
    19501 caggcgctga ataaatcact gatgaaaacc gcgcctggcg agaaagaagt gatcgacctg
    19561 gtcgattact tacttaccag ttgctttgtc accggacgca gtttcccact tgatggcggt
    19621 cgtcatctgc gttaatgcag ctttatccag catataatca acacccatgc aacacgcaac
    19681 agaaccaaca aaagcggcgg gcaagcgcat cattccggtg aaataccaca gcgacaccag
    19741 gtaaacaaaa tagggaataa tcgaccacat actaaaaatg atggttgcgc gtaaggcttc
    19801 aatgccgcgt tcgctggcaa caatataatg cgcgataagc gcaaaggtcg ggaaaagtgg
    19861 aatcagcccg gcgatataat aatttttcgt ttttgctaaa acaccaatca acagcactac
    19921 cagcgcaccg agcgcggctt tgattttacc ttaacacttc cataacaagt catcagtaga
    19981 atacctgatg aaaacttgtt tagaaacgat tgatagtaag taaaaacagc gcggtgtatt
    20041 gtgacgtttt tatatctacc gtgaatgtta tgaacactat cgtatttgtg gaagatgatg
    20101 cggaagtcgg ttcactgatt gccgcgtacc tggcaaaaca tgatatgcag gttaccgtag
    20161 agccgcgcgg cgaccaggcc gaagaaacca ttttgcgaga aaatccggat ttggtgttac
    20221 tcgacatcat gctaccaggc aaggacggca ttaccatttg tcgtgattta cgcgcaaagt
    20281 ggtctggacc gattgttctt ctaacctctc tcgatagcga tatgaaccac atcctggcac
    20341 tggaaatggg tgcctgcgac tatattctca aaacgatgcc ccctgctgtt ttgctagcgc
    20401 gtttacgttt gcatttgcgt cagaatgagc aagccacact gaccaaaggt cttcaggaaa
    20461 cgtctctgac tccctacaaa gccctgcatt tcggcacgtt gaccatcgat cccatcaacc
    20521 gcgtagtcac cctggctaac actgaaatct cgctctcgac agctgatttc gaattattgt
    20581 gggaattagc tacccatgcc gggcaaatca tggaccgcga tgcattgctg aaaaatttac
    20641 gcggcgtcag ttatgacgga ctggatcgta acgtggacgt ggctatttcg cggttaagaa
    20701 aaaaactgct cgataacgcc gcagaacctt atcgcattaa aactgtgcgt aacaaaggct
    20761 atctttttgc gcctcatgca tgggaataag cgatgaaaaa actgtttatc cagttttacc
    20821 tgttattgtt tgtctgcttc cttgtgatgt ctctgctggt tgggttggtg tacaaattta
    20881 ccgccgaacg cgcgggcaaa cagtcgctgg atgatttgat gaacagttcg ctgtatctga
    20941 tgcgcagcga attgcgtgag atccccccac acgactgggg taagacgctg aaagagatgg
    21001 atttaaatct ctctttcgat ctgcgtgtcg agccactgag taaataccat cttgatgata
    21061 tttccatgca ccgactgcgt ggcggcgaaa ttgtcgccct ggacgatcag tacacgtttt
    21121 tgcagcgtat cccgcgcagc cactacgtgc tggcagttgg tcctgttcct tatctttatt
    21181 atctccatca gatgcgattg ctggatatcg ccctgatcgc ttttattgct atttccctcg
    21241 cctttccggt gtttatctgg atgcgtccgc actggcagga tatgttaaaa ctggaagcag
    21301 cggcgcaacg atttggcgat gggcatctct atgagcgtat ccactttgat gagggttcga
    21361 gctttgaacg acttggtatc gcatttaacc agatggcgga caatatcaac gccttgattg
    21421 ccagcaaaaa acagcttatt gacggtatcg ctcacgaact gcgaacaccg ttagtgcgcc
    21481 tgcgttatcg actggagatg agcgataacc tgagcgccgc cgagtcccag gcgttgaatc
    21541 gtgatatcag tcaacttgaa gctttaattg aagagctgct gacttatgcc cgactcgatc
    21601 gcccacaaaa cgagcttcat cttagcgaac cagacctgcc gttgtggctg tcaacgcatc
    21661 tggcagatat tcaggcagta acgcccgata aaacggtacg gataaaaacg ctcgtgcaag
    21721 gccattatgc ggcgttggat atgcgcttaa tggagcgcgt gctggataat ttgctcaata
    21781 acgccctgcg gtactgccat tcaacggttg aaaccagcct gctactgtcg gggaatagag
    21841 cgacattaat tgttgaggat gatggcccag ggattgcccc agaaaaccgc gaacatatct
    21901 ttgaaccttt tgttcgcctc gatcccagcc gggatcgctc aaccggcggc tgcgggctgg
    21961 ggctggcaat tgtccactct atagcactgg caatgggcgg tacggttaat tgtgacacca
    22021 gcgaactggg tggtgctcgc ttctcgttta gctggccgtt atggcataac atcccgcaat
    22081 ttacctctgc ctgacactac gcgcacgatg gtcaagtcac caccattgtg ctataaatta
    22141 aggatgttgt aactaaagtg gttaatatta tggcgcgtta cgatctcgta gaccgactca
    22201 acactacctt tcgccagatg gaacaagagc tggctgcatt tgccgctcat cttgagcaac
    22261 acaagctatt ggttgcccgc gtgttctctt tgccggaggt aaaaaaagag gatgagcata
    22321 atccgcttaa tcgtattgag gtgaaacaac atctcggcaa cgacgcgcag tcgctggcgt
    22381 tgcgtcattt ccgccattta tttattcaac aacagtccga aaatcgcagc agcaaagccg
    22441 ctgtccgtct gcctggcgtg ttgtgttacc aggtcgataa cctttcgcaa gcagcgttgg
    22501 tcagtcatat tcagcacatc aataaactca agaccacgtt cgagcatatc gtcacggttg
    22561 agtcagaact ccccaccgcg gcacgttttg aatgggtgca tcgtcatttg ccggggctga
    22621 tcacccttaa tgcttaccgc tcgctcaccg ttctgcacga ccccgccact ttacgttttg
    22681 gctgggctaa taaacatatc attaaaaatt tgcattgtga tgaagtcctg gcacagctgg
    22741 aaaaaagcct gaaatcacca cgcagtgtcg caccgtggac gcgcgaggag tggcaaagaa
    22801 aactggagcg agagtatcag gatatcgctg ccctgccaca gaacgcgaag ttaaaaatca
    22861 aacgtccggt gaaggtgcag ccgattgccc gcgtctggta caaaggagat caaaaacaag
    22921 tgcaacacgc ctgccctaca ccgctgattg cactgattaa tcgggataat ggtgcgggcg
    22981 tgccggacgt tggtgagttg ttaaattacg atgccgacaa tgtgcagcac cgttataaac
    23041 ctcaggcgca gccgcttcgt ttgatcattc cacggctgca cctgtatgtt gcagattaac
    23101 gcccggcttt catactgccg accatctgtt ctggccgtac ccagctgtca aactcggctt
    23161 cgctaagata ccccagcgca agggccgcag cttttaaggt cagcccttct ttatgcgctt
    23221 ttttggcgat ctccgcggct ttgtcataac caatgtgggt gttaagcgca gtcaccagca
    23281 tcagcgattc attgagtaat tgattgattc gctcacgatt cggttcaata cccactgcgc
    23341 agtgtttgtt aaaactttcc atgccatctg ccagcaagcg caccgattgc aggaaattat
    23401 ggatcaccat cggacggaag acgttcagtt caaagttacc ggaagcgcca cccatgttga
    23461 tcgccacgtc gttccccatt acctgacagc agagcatggt taatgcttca cactgggttg
    23521 ggttcacttt ccccggcatg attgagctgc ccggctcatt ttccgggatt gagatttcac
    23581 caattccgca gcgcgggcca gaggccagcc agcggacatc attggcgatt ttcatcagtg
    23641 acgcagccaa ccctttcaac gcgccgtgcg cctgaaccag ggcatcacag gtcgcaagcg
    23701 cttcaaattt gttcggcgcg gtaacaaacg gtgcacaggt aatgactgcc agttcatctg
    23761 ctacgcgacg cgcatactcc ggatgggtat ttagtccagt acccaccgct gtaccgccaa
    23821 gagccagttc cgctacgtga ggcaggctgt attcgatatg tttgagatta tgctcgagca
    23881 tcgctaccca gccggaaatc tcctgcccta gtgttagcgg cgtggcgtcc tgcaagtggg
    23941 ttcgaccgat tttgacgata tcggcaaatg cacgcgattt ttcactcagt gtctgcgtca
    24001 gggttttaag ctgcggaatg agttgcttgc gcagcgccag cagcgccgca acgtgcatcg
    24061 ccgtcggaaa gacatcgttg gaactttggc ttttgttcac gtcgtcgtta gggtgaactt
    24121 tacgttccat cccgcgcacg ccgccgagta attcactggc ccggttagcc agcacttcgt
    24181 tcatgttcat gttactttgc gtgccggagc cggtctgcca gatagccagc gggaattcgt
    24241 cgtcatgctg tcctgccagt acttcatccg ccgcctgccg aatggcgctc gctttctctt
    24301 cagacaacaa gcctaaatct tcattaactt ttgccgctgc gcgcttggtt agcgccagcg
    24361 catgaatcag tgaggtgggc attttctccg tcgaaatgcg gaaatgctcc agcgagcgtt
    24421 gagtttgtgc gccccacagc ttatctgccg ggacatcaat cgcccccatc gaatcttttt
    24481 cgctgcgtac tgtattcatg gcctgctcct cacctgttta attttttctt tctgttttgc
    24541 tttcgctaag caacttttag ctcacttatt atttactatt tgataacaag tgtttggtct
    24601 ttcgtgccat gtaaaaaaac cgccccgaag gg
//
LOCUS       DAJTMC010000049        24556 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.049, whole genome shotgun sequence.
ACCESSION   DAJTMC010000049 DAJTMC010000000
VERSION     DAJTMC010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24556
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.049"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(148..3003)
                     /gene="valS"
                     /locus_tag="OO130_001748"
     CDS             complement(148..3003)
                     /gene="valS"
                     /locus_tag="OO130_001748"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HCR5365196.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVSL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGADVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(3003..3446)
                     /gene="holC"
                     /locus_tag="OO130_001749"
     CDS             complement(3003..3446)
                     /gene="holC"
                     /locus_tag="OO130_001749"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HCR5365197.1"
                     /translation="MKNATFYLLDNDTTVDGLSTVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            complement(3606..5117)
                     /gene="pepA"
                     /locus_tag="OO130_001750"
     CDS             complement(3606..5117)
                     /gene="pepA"
                     /locus_tag="OO130_001750"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HCR5365198.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAILHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            5384..6484
                     /gene="lptF"
                     /locus_tag="OO130_001751"
     CDS             5384..6484
                     /gene="lptF"
                     /locus_tag="OO130_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HCR5365199.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            6484..7566
                     /gene="lptG"
                     /locus_tag="OO130_001752"
     CDS             6484..7566
                     /gene="lptG"
                     /locus_tag="OO130_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HCR5365200.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQTFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(7727..9229)
                     /locus_tag="OO130_001753"
     CDS             complement(7727..9229)
                     /locus_tag="OO130_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HCR5365201.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLTRLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEKQGAAHFFGEPMLDIKDWMRTDTNGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDDVDRESAYEMLQKGFQASTKQQNNPPAKGKAVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRRR"
     gene            complement(9359..10378)
                     /gene="ahr"
                     /locus_tag="OO130_001754"
     CDS             complement(9359..10378)
                     /gene="ahr"
                     /locus_tag="OO130_001754"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HCR5365202.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELRPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALSGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSISGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIKHVRDGKARYRVV
                     LKADF"
     gene            10574..10658
                     /locus_tag="OO130_001755"
     tRNA            10574..10658
                     /locus_tag="OO130_001755"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10608..10610,aa:Leu,seq:caa)
     gene            10822..12087
                     /locus_tag="OO130_001756"
     CDS             10822..12087
                     /locus_tag="OO130_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCR5365203.1"
                     /translation="MKLNARQVETAKPKDKTYKMADGGGLYLEVSAKGSKYWRMKYRR
                     PSDKKEDRLAFGVWPTVTLAQARAKRDQAKKLLAQGIDPKAEQKEAQAENSGAYSFET
                     IAREWHASNKRWSEDHRSRVLRYLELYIFPHIGSSDIRQLKTSHLLAPIKKVDASGKH
                     DVAQRLQQRVTAIMRYAVQNDYIDSNPASDMAGALSTTKARHYPALPSSRFPEFLARL
                     AAYRGRVMTRIAVELSLLTFVRSSELRFARWDEFDFDKSLWRVPAKREEIKGVRYSYR
                     GMKMKEEHIVQLSRQAMILLNQLKQISGDKELLFPGDHDATKVMSENTVNSALRAMGY
                     DTKTEVCGHGFRTMARGALGESGLWSEDAIERQLSHSERNNVRAAYIHTSEHLDERRL
                     MVQWWADYLQANKGKTITPYDFAKINKSR"
     gene            complement(12142..13152)
                     /locus_tag="OO130_001757"
     CDS             complement(12142..13152)
                     /locus_tag="OO130_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5365204.1"
                     /translation="MIYITSESKETLMIKYEGKMKKNIRVIPKNIHSKLRRLGNTVVA
                     GTSIAFTENQLKSGALEHLGIYFDNGVNAYETSVIPDPLQGKYSLKNVFGEEIVRKDL
                     PKETHYTEIESPNWGDSSNGTHTVRLPYEKYPRDIIPPTLIAIEINHKQPSDSHFIFN
                     FRVTRILDKQSNKFDDELLFDLNLLQENLGKCGIEDADKPISTYADTLIVSWEIFPPG
                     SKEETLARIFRGKNITSDKKNVAENRYDFFMSLEPKKIVTGNSTFSNYIGAMLEDDLV
                     VFENIEYGNAIYILYDNWDDISKLSRIDLLSGRAGSNFDRIIHSGNWKDEVRKKVAAG
                     RL"
     gene            complement(13103..13639)
                     /locus_tag="OO130_001758"
     CDS             complement(13103..13639)
                     /locus_tag="OO130_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004152200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5365205.1"
                     /translation="MLKAIAGIAKYTWIYMAIFIISLMIISSSDINYVYNDFANYCTL
                     LVSVSGMVFTIMGIWLAFLYPNALQRIVNPKTIETVDFTEQLEDTRRLEAIVGSVLKS
                     SFVMITLLVVYLVKLVFFKSHLYLESQDVIKNTSLALIIAISYMQIEAVISVILANVM
                     FINDLHNKREQRDIDDQV"
     gene            complement(13640..14719)
                     /locus_tag="OO130_001759"
     CDS             complement(13640..14719)
                     /locus_tag="OO130_001759"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5365206.1"
                     /translation="MNVGHLNFFKVNKCGLYKVNDDNTYGLELSETFDLIQDWVGTKS
                     LALTIPWDPKEKPNSSKCYCKDIYKDENTGDFLVMLWKSDTDSTGSLLGASEDGEIGS
                     SSVVKYTNSYRGKKVIWGRPCFYWVIPELETIVSIKFDHSVCDSELFQDYVHSSITNR
                     VKHSKRVKNKTEKGYIRLSNTDDEDLYKYMYRFDMKLRSLETTHTELGKLIPKITHIV
                     RRETIIINPNDARADWLKTFSTLVPFVSGKKNAKTRQIEIKAEAKPSLNEVQEIIEKY
                     SAEDREKRLWDNVGFATDKGITWVDKYRMRDILNVPSDDYSTYSAAYIYEQISHKRKE
                     FISPILKELKALQSQPRIKKASGED"
     gene            complement(15117..15689)
                     /locus_tag="OO130_001760"
     CDS             complement(15117..15689)
                     /locus_tag="OO130_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Polarity suppression protein"
                     /protein_id="HCR5365207.1"
                     /translation="MESTALQQAFDTCQNNKAAWLQRKNELAAAEQEYLRLLSGEGRN
                     VSRLDELRNIIEVRKWQVNQAAGRYIRSHEAVQHISIRDRLNDFMQQHGTALAAALAP
                     ELMGYSELTAIARNCAIQRATDALREALLSWLAKGEKINYSAQDSDILTTIGFRPDAT
                     SVDDSREKFTPAQNMIFSRKSAQLASRQSV"
     gene            complement(15763..16263)
                     /locus_tag="OO130_001761"
     CDS             complement(15763..16263)
                     /locus_tag="OO130_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HCR5365208.1"
                     /translation="MIYCPSCGHVAHTRRAHFMDDGTKIMIAQCRNIYCSATFEASES
                     FFSDCKDSGMEYISGKQRYRDSLTSASGSMKRPKRMLVTGYCCRRCKGLALSRTSRRL
                     SQEVTERFYVCTDPGCGLVFKTLQTINRFIVRPVTPDELAESLHEKQELPPVRLKTQS
                     YSLRLE"
     gene            complement(16260..16994)
                     /locus_tag="OO130_001762"
     CDS             complement(16260..16994)
                     /locus_tag="OO130_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001600374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoprotein 3"
                     /protein_id="HCR5365209.1"
                     /translation="MSDNTIPEYLQPALAQLEKARAAHLENARLMDETVTAIERAEQE
                     KNALAQADGNDADDWRTAFRAAGGVLSDELKQRHIERVARRELVQEYDNLAVVLNFER
                     ERLKGACDSTATAYRKAHHHLLSLYAEHELEHALNETCEALVRAMHLSILVQENPLAN
                     TTGHQGYVAPEKAVMQQVKSSLEQKIKQMQISLTGEPVLRLTGLSAATLPHMDYEVAG
                     TPAQRKVWQDKIDQQGAELKARGLLS"
     gene            17546..17812
                     /locus_tag="OO130_001763"
     CDS             17546..17812
                     /locus_tag="OO130_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015979398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCR5365210.1"
                     /translation="MQAVFSSPSPAPVTPLMPLPDITQERFLRLPEVMHLCGLSRSTI
                     YELIRKGEFPPQVSLGGKNVAWLHSEVTAWMAGRIAGRKRGYDA"
     gene            17809..18408
                     /locus_tag="OO130_001764"
     CDS             17809..18408
                     /locus_tag="OO130_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000980235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HCR5365211.1"
                     /translation="MMMPALQKLPFSGLPFSGICGYSFSAAAKSAAGRENPSNSQATY
                     DAPRVFFCVAINATERQIMVWCVVSRADGIPCILPASAHYAAESMVAQAGQPPGWPVS
                     CEAGILTPVWAIAIERENSGDSVICYSQEAAIMATTLTPSHPEFVFVFAAVRRADRHP
                     RICMLRTVAGDERSARRSLVRDYVLSLAARLPVVEVSRA"
     gene            18401..18688
                     /locus_tag="OO130_001765"
     CDS             18401..18688
                     /locus_tag="OO130_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Derepression protein"
                     /protein_id="HCR5365212.1"
                     /translation="MRNKKAPQTVSARHDAREHLSIEAYHKLNRASAVSQFVGGDLIH
                     RELSGLHQLYIPHIFSYLNEDIDFVLNELKAKGLCRDFLAQQKDRGDRTHV"
     gene            18681..19136
                     /locus_tag="OO130_001766"
     CDS             18681..19136
                     /locus_tag="OO130_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016569899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5365213.1"
                     /translation="MFDFPQPGEIYRSAGFPDVAVVGILEDGIPWEMPYRCPDIVWNP
                     YRRKFSILVRILADGRTTDIPLGRFLREFTCDRPDLFKRSPVNRHAVLKEMAGDPELQ
                     KWREKYQDIYPQDTVPVSRAAPVAREWREIPRTEPDPEITPDNSYRNYL"
     gene            19272..19592
                     /locus_tag="OO130_001767"
     CDS             19272..19592
                     /locus_tag="OO130_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5375 domain-containing protein"
                     /protein_id="HCR5365214.1"
                     /translation="MKTPLPPVLRAALYRRAVACAWLTVCERQHRYPHLTLESLEAAI
                     AAELEGFYLRQHGEEKGRQIACALLEDLMESGPLKAAPSLSFLGLVVMDELCARHIKA
                     PVLH"
     gene            19607..21940
                     /locus_tag="OO130_001768"
     CDS             19607..21940
                     /locus_tag="OO130_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000783678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HCR5365215.1"
                     /translation="MKMNVTATVSHALGHWPRILPALGIQVLKNHHQPCPVCGGSDRF
                     RFDDREGRGTWYCNQCGAGDGLKLVEKVFGVSPSDVAAKVAAVTGSLPPADPAVTAAA
                     VAETDAARKNAAALAQTLMAKTRPGTGNAYLTRKGFPGRECRMLTGTHRAGGVSWRAG
                     DLVVPLYDDSGELVNLQLISADGRKRTLKGGQVRGTCHTLEGQNQAGKRLWIAEGYAT
                     ALTVHHLTGETVMVALSSVNLLSLASLARQKHPACQIVLAADRDLSGDGQKKAAAAAD
                     ACEGVVALPPVFGDWNDAFTQYGGEVTRKAIYDAIRPPAESPFDTMSEAEFSAMSTSE
                     KAMRIYEHYGEALAVDANGQLLSRYENGVWKVLPPLDFARDVAGLFQRLRAPFSSGKV
                     ASVVDTLKLIIPQQEAPSRRLIGFRNGVLDTQNGTFHPHSPSHWMRTLCDVDFTPPVE
                     GETLETHAPAFWRWLDRAAGGRAEKRDVILAALFMVLANRYDWQLFLEVTGPGGSGKS
                     IMAEIATLLAGEDNATSATIETLESPRERAALTGFSLIRLPDQEKWSGDGAGLKAITG
                     GDAVSVDPKYRDAYSTHIPAVILAVNNNPMRFTDRSGGVSRRRVIIHFPEQIAPQERD
                     PQLKDKITRELAVIVRHLMQKFSDPMLARSLLQSQQNSDEALNIKRDADPTFDFIGYL
                     ETLPQTSGMYMGNASIIPRNYRKYLYHAYLAYMEANGYRNVLSLKMFGLGLPVMLKEY
                     GLNYEKRHTKQGIQTNLTLKEESYGDWLPKCDDPATT"
     gene            22106..22300
                     /locus_tag="OO130_001769"
     CDS             22106..22300
                     /locus_tag="OO130_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR5365216.1"
                     /translation="MWIKDALVWIRATDPNLLIKNYLTFRFIDGLKIDWSEKQWGHKR
                     GHISLLCFIICFLSLTYFDI"
     gene            22518..23786
                     /locus_tag="OO130_001770"
     CDS             22518..23786
                     /locus_tag="OO130_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCR5365217.1"
                     /translation="MALTDTKVRSAKPEEKEYSLVDGDGMSLLVKPGGSKYWRFRFRF
                     GGKQHLMAFGVYPDVSLADARKKREEARKLVAAGIDPREHKRAVKEEQAKEIITFEKV
                     AREWLVTNQKWSEEHANRVKKSLEDNIFPAIGSHNIAELGTRDLLIPIKAVEKSGRLE
                     VASRLQQRTTAIMRYAVQSGLIDYNPAQEMAGAVASSNRQHRPALELKRIPELLQKID
                     EYTGRPLTRWATELTLLIFIRSSELRFARWSEIDFETSMWTIPPEREPIPGVKHSQRG
                     AKMRTPHLVPLSKQALAILKQIKQFCGEHELIFIGDHDPSKPMSENTVNSALRVMGYD
                     TKVEVCGHGFRTMACSSLIESGLWSKDAVERQMSHMERNSVRAAYIHKAEHLDERKLM
                     LQWWADFLDANREKGITPFDYAKINRGNGE"
     gene            24177..>24556
                     /locus_tag="OO130_001771"
     CDS             24177..>24556
                     /locus_tag="OO130_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001009620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5365218.1"
                     /translation="MNFKKKLEEHFKQFEASPVLFVGSGVSRRYLGVPCWQDLLKHFA
                     EAIGENHIKLKTKSNGDLPEYAQLLVSAYAEKWWDTEEGQLALSEKEQEKTFINEQSP
                     LKLSISKYIENAHKNIIDNDELKH"
BASE COUNT         5831 a         6368 c         6100 g         6257 t
ORIGIN      
        1 accttccttt aatcaataaa gaagaacaac cagataaagg cttcttagcc agtatttcac
       61 ggggaattat gccttcttta ccatttctgt aagagataaa aaaggccgga gcatgctccg
      121 gccttcgttt tcatcactgt gttttgatta cagcgcggcg ataacagcct gctgttcaat
      181 cagcttcgct ttcgcttctg catagccttc cagcttctca cgctctttcg cgatgaccgc
      241 ttccggtgcg cgggcgacaa agccttcatt cgccagcttg ttctcgatac ggctgatttc
      301 gccttcgatc ttcgccactt ctttcgccag acgcgccagc tcatcttctt tgttgatgag
      361 gccagccatc gggatcagca gctctgcgcc gtcgatgatc ttcgtaacgg aaaccggacc
      421 tttgtcatcg gcaggcagca cggtgatgct ttccagacgc gccagggttt gcaggaagcc
      481 acggttttca tttacgcgac gttctgcatc cgcgctgcaa ccacgcagca gcagctccag
      541 cggtttgccc ggcgcgatgt tcatttctgc acggatgtta cgtaccgcaa cgatcgcctg
      601 tttcagccac tcggtgtcag ccagtgcggc ttcatcaacc tgagatgcat cgtactgcgg
      661 gaacggctgc agcatgatgg tgtcggcagt gataccgcaa agtactttca cacgctgcca
      721 gatggtttcg gtgatgaacg gaatgatcgg atgcgcgagg cgcagcagac cttccagtac
      781 agtcaccagc gtatggcgag taccgcgcag ttcggcttcg gttccaccgt tcattaccgg
      841 cttagtcagc tccagatacc agtcgcagaa ctggttccag gtgaactcat acagaatgcc
      901 tgcagcgata tcgaagcgga agctgtccag cgcttcgcgg tacgctttga tggtctggtt
      961 gaactctgcc agaatccagc ggtccgccag cgacagcgtc atttcaccac cgttgaagcc
     1021 gcaatcctga ccttccgtgt tcatcagcac aaagcggctg gcgttccaca gcttgttaca
     1081 gaagttacgg tagccttcca ggcgtttcat atcccagttg atgtcacgac cggtagaagc
     1141 cagcgccgcc agggtgaagc gcagggcatc ggtgccgtgc ggctcaatgc cgttcgggaa
     1201 ctgtttctcg gtgcgcttac ggattttgtc cgccagctgc ggctgcatca tattgccggt
     1261 acgtttctcc agcagttccg gcagcgaaat accgtcaacc atatccagcg ggtcaataac
     1321 gttacccttg gatttggaca tcttctggcc ttcgtcgtca cgaatcaggc cggtcatgta
     1381 aacggtgtgg aacggcacct gcggtttgcc attttcatct ttgatgaagt gcatggtcat
     1441 catgatcatg cgggcaatcc agaagaaaat gatgtcgaaa ccagatacca tcacgctggt
     1501 tgggtggaac tggcgcagtg cgtcggtgtt ttccggccag cctagggtag agaaggtcca
     1561 cagcgcagaa gagaaccagg tgtcgagaac gtcttcgtcc tgacgcaggg caacgtcggc
     1621 accgaggtta ttttccttgc gcacttcttc ttcgttgcgg ccaacataaa cgttacctgc
     1681 ttcgtcgtac catgcaggga tacggtgacc ccaccacagc tgacgagaga tacaccagtc
     1741 ctgaatatcg cgcatccagg agaagtacat gttttcgtac tgcttcggta cgaactgaat
     1801 gtcgccgttc tcaaccgcct caaccgccgg tttcgccagt acatcggcac gcacgtacca
     1861 ctggtcggtg agcatcggtt cgataactac gccgccacgg tcgccgtaag gcacggtcag
     1921 gtcgtgcggt ttaatttctt ccagcaggcc gagcgcgtca accgcagcaa cgactgcttt
     1981 acgtgcagca aaacgctcca gtttctggaa ctctgccggg atttcgctgg aataaacgtc
     2041 agattcgtta cctttggtat cgaacacctg ggcgctttca cggatatcgc cgtcaaaggt
     2101 caggatgttg atcatcggca gcgcgtgacg tttacccact tcatagtcgt taaagtcgtg
     2161 cgccggagtg attttcacgc agccggtgcc tttttccatg tcggcgtgtt cgtcgccaac
     2221 gatcggaata cgacggttaa ccagcggtag aatgacatat ttgccaatca ggtctttgta
     2281 acgcggatct tccgggttaa cggccacgcc ggtatcgccc aacagggttt ccggacgggt
     2341 agtcgcaacc accagataat ctttaccgtc tgcggttttc gcgccgtcag ccagcggata
     2401 gcggatgtgc cacatcgaac ctttcgattc gcggttttcc acttccaggt cagagatagc
     2461 ggtgcgcagt ttcggatccc agtttaccag gcgtttgcca cggtaaatca ggtcttcttt
     2521 atacagacta acgaaaactt ctttcaccgc attggacagg ccttcgtcca tcgtgaagcg
     2581 ttcacgctcc cagtcgacgg agttgccgag acggcgcatc tgacgggtaa tcgtgccgcc
     2641 ggattccgct ttccattccc aaattttgtc gatgaaagct tcgcggccgt agtcgtgacg
     2701 ggttttacct tcttctgcgg caatcttgcg ctcaacgacc atctgggtag cgatcccggc
     2761 gtggtcagta ccgacctgcc acagggtgtt tttgccctgc atgcgctgat agcggatcat
     2821 ggtatccatg atggtttgct ggaaggcgtg acccatatgc aaactgccgg tgacgttcgg
     2881 cggcgggatc atgatgcaga aactttcctg gctttcatcg ccattaggct taaagtagcc
     2941 ctgcttttcc cagtgctcgt aaagcggctg ttcgatatct tgtgggttat atgtcttttc
     3001 cattatttcc aggttgccgt attcaggttg aaaccagcca cgcggtaggc tttatagcgt
     3061 tcgcgcgcca gttgtttcag agaatcttca taaggaacga agtctaccac ttctgtgaaa
     3121 gcggtggcaa aatctgcaaa gcttgttcgc agactaatca atatatcgcg cgggctgctg
     3181 ctgcgttttt gcggccaggc gatctccacc ggcgcaccgc cgcgcggtcc ttctcccgct
     3241 aaattatgcg gaacaaagct ttctgccgga cgcgcccaca gggcttcatc cagccggtaa
     3301 gcctgctttt catcttcaca ggcgatgagc acgcgcttac cgctgcgcca acgttctgcg
     3361 gcaatttcac acaccagttg ctcaacggtg cttaagccat cgacggtggt gtcattgtcc
     3421 agaaggtaga acgtcgcgtt tttcatatat ggggcttctt gtggtggatt taaatacgca
     3481 tcaggtatac accgcagcgc ctgatgcgac gctgttgtgt cttatcaggc ctacgagttc
     3541 agtgctgtgt aggtcggata aggcgttcac gccgcatccg gcaataacag ccttgcctga
     3601 cgcaattact cttcgccgtt aaacccagcg cggtttaaca ggaactgtgc cagcaacgcc
     3661 accggacgac cggtggcgcc ttttgcttta ccagaacgcc aggcggttcc ggcgatatcc
     3721 aggtgcgccc agttgtactt acgggtaaag cgcgacagga agcaacctgc ggtaatcgcc
     3781 ccaccaggac gaccgccaat gttcgccata tcggcaaaat tggactccag ctgttcctga
     3841 tactcgtcac ccagcggtaa gcgccatgcg cggtcaccgg attgttcaga cgcggcaatc
     3901 agttcatggg ccagcggatt atggttcgcc atcagaccgg taatgtgatg acccagcgcg
     3961 atcacgcagg caccggtcag cgtcgccacg tcaatcaccg cttccggctc aaaacgctca
     4021 acgtaagtta acacgtcgca cagtaccagg cggccttcag cgtcggtatt cagcacttca
     4081 acagtttgac cggacatggt ggttaacaca tcgcccggac gataggctcg tccgccaggc
     4141 atgttttcgc agcctgccaa cacgccgata acgttaatcg gcagttgtag ctccgcgacc
     4201 atccgcatca cgccgtaaac cgctgccgca ccgcacatat cgtacttcat ctcatccatg
     4261 ccttctgaag gcttgatcga gataccgccg gagtcgaagg ttaaaccttt acccaccagc
     4321 acgattgggc gtgcatcttc cgacgcgttg cctttgtact caatcaccga catcagcgat
     4381 tcgttttgcg aaccctgacc gaccgccaga taggaatgca tccccagctc tttcatctgc
     4441 tgttcgccga taacgcgggt gatgacattc ttgctgtagc tgtcagccag ctggcgcgct
     4501 tgtgaagcga ggtaagcggc gttacagata ttcggcggca tattgccgag atcttttgct
     4561 gctttaatcc cggcggcaat cgccagaccg tgcaggatcg cgcgctcacc gctggtcagt
     4621 tcacggcggg tcggcacgtt gaacaccatt ttacgcagcg gacgacgcgg ttcgctcttg
     4681 ttcgttttca gctgatcgaa actgtagagc gtctcttttg ccgtctcgac agcctgacgc
     4741 actttccagt agttgttacg gcctttaacg tgcagttcag tcagaaagca gaccgcttcc
     4801 attgagccag tatcattcag cgtattaatg gttttctgaa taacctgctt gtactgacgc
     4861 tcatccagct cacgttcttt gccgcaacca ataaggagaa ttcgctcgga aagtacattc
     4921 ggaacatggt gcagcaacaa tgtctgcccc ggttttcctt ccagttcgcc ccgacgtagc
     4981 agggcgctga tgtacccatc gctgatttta tcgagctgtt ctgcaatcgg agaaaggcga
     5041 cgtggttcga agacgcccac gacgatgcag gcactccgct gtttctccgg gctaccgctt
     5101 tttacactaa actccatgca ctacgctcct gaatcttaaa gacaacggcg gtggctacag
     5161 atagaattgc aagctttcgt aactcatgtc cgctgttgcg atgacttcgt gttaatctta
     5221 acgttattac ggcattggca cgtcagaaca aaatctgaga ggtgaatccg ttgagtataa
     5281 ttatcttagc gacgatttcg acgactcaag agaataaatg acgtttaagc catgaaacaa
     5341 gctaaaatcc tgcaaaagac gagtttttac gggcgtattt aaagtgataa tcataagata
     5401 tctggtgcgg gagacgctca aaagccagct ggcgatactc ttcatcttgc ttttgatctt
     5461 cttctgtcaa aagttagtga ggatcctcgg cgcagcggtt gacggcgata ttccggcgaa
     5521 tctggtgctc tcccttctcg ggttgggcgt gccggaaatg gcgcagctta tcctgccatt
     5581 aagcctgttc ctcgggctgc tgatgacgct gggcaaactg tataccgaaa gtgaaattac
     5641 ggtaatgcat gcctgcggcc tgagcaaagc ggttctggtg aaagcggcaa tgatccttgc
     5701 ggtattcact gcaatcgtcg cggcggttaa cgtgatgtgg gcgggaccgt ggtcatcgcg
     5761 tcatcaggat gaagtgttag cagaagcgaa agcgaaccct ggcatggcgg cgctggcgca
     5821 agggcaattc cagcaagcga ctaatggcag ctcggtgctg ttcatcgaaa gcgttgacgg
     5881 cagcgatttc aaagatgtgt tcctcgcgca aattcgacca aaaggtaatg cacgtccttc
     5941 tgtggtggtg gccgattccg gacatttaac ccagctgcgc gacggctccc aggtcgtcac
     6001 tctcaaccag ggaacgcgct tcgaaggcac tgcactgtta cgtgatttcc gcattacgga
     6061 cttccaggat tatcaggcga tcattggtca ccaggcggta gcgctcgacc cgaacgatac
     6121 cgaccagatg gacatgcgta cattgtggaa cactgacacc gatcgtgccc gcgcagaact
     6181 gaactggcgt atcacgttgg tattcaccgt gtttatgatg gcacttatgg tcgtaccgct
     6241 gagcgtggtt aacccgcgtc agggacgcgt actatcgatg ctgccagcca tgctgctgta
     6301 tctacttttc ttcctgatcc agacctccct gaaatcgaac ggcggtaaag gtaagctgga
     6361 cccgacgctg tggatgtgga ccgttaacct gatttatctg gctttggcga ttgttctcaa
     6421 cctttgggac accgtgccgg tccgccgcct gcgcgccagt ttttcgcgta aaggagcggt
     6481 gtgatgcaac cttttggcgt acttgaccgc tatatcggta aaactatttt caccaccatc
     6541 atgatgacgc tgttcatgct ggtgtcgctg tcgggcatta tcaaatttgt cgatcagctg
     6601 aaaaaagccg ggcaggggag ttacgacgcg ttaggcgcag gaatgtatac cttgctgagc
     6661 gtgccgaaag atgtgcaaac attcttcccg atggcggctc tgcttggggc gttgcttggt
     6721 cttgggatgc tggcgcagcg cagcgaactg gtggtgatgc aggcttctgg tttcacccgt
     6781 atgcaggttg cgctgtcggt gatgaaaacc gccattccgc tggtcttgct gacgatggcg
     6841 attggtgaat gggtcgcgcc gcagggcgag cagatggcgc gtaactaccg tgcgcaggcg
     6901 atgtacggcg gctcgttgct ctctacccag caaggcttat gggcgaaaga tggcaacaac
     6961 ttcgtttaca ttgagcgggt taaaggtgac gaagagttag gtggcatcag tatttatgcc
     7021 tttaacgaga atcgtcgtct gcaatccgta cgctatgccg ctactgcgaa gtttgacccg
     7081 gaacataaag tctggcgtct gtcgcaggtt gatgaatctg atctgaccaa tccgaaacag
     7141 atcaccggtt cgcagacggt gagcggcacc tggaaaacca acctcacgcc ggacaaactg
     7201 ggcgtggtgg cgctggaccc ggatgcactc tcaatcagcg gtttgcacaa ctacgtgaag
     7261 tatctgaagt cgagcggtca ggatgccgga cgttatcagc tcaatatgtg gagcaaaatc
     7321 ttccagccgc tatccgtggc ggtgatgatg ctgatggcgc tgtcgttcat ctttggccca
     7381 ctgcgtagcg taccgatggg cgtgcgtgtg gtcaccggta tcagcttcgg ttttgtcttc
     7441 tacgtactgg accagatctt cggcccgctg acgttggttt atggcatccc gccgatcatc
     7501 ggcgcactgt tgccaagcgc cagcttcttc ttaatcagcc tgtggctgtt aatgagaaaa
     7561 tcgtaaccga atgaacgaat aaagggagcg aaagctccct ttattattgt tagcaaagtg
     7621 tgcttcgttc atgcctgaaa aatcatgaaa atccaatata ttgcagtggt gtaggcatga
     7681 taagacgcgc cagcgtcgca tcaggcaaag acaaatctcg cctgacttac cttcttcttc
     7741 cccccagcaa actccccaac atcccacgta caatctgatt cgtcacctgg cgggcggcgc
     7801 ttttcgccat tgtttgcacc acaccatctt tcttcccgcc gcgtggtccg gtagtgccaa
     7861 acaaaatatc cttcaatcca ccaagaatac cgtcatccac cgccaccgct ttccctttcg
     7921 cgggaggatt attttgctgc ttggtactgg cctgaaagcc tttttgcaac atctcatagg
     7981 cggattcccg gtccacgtca tcctcgtact tgccatacac cggggagtgg ttaatcaagc
     8041 cattgcgctc atcttccgtc accggtccca tccgcgaaca aggcgcaatc accatcgccc
     8101 gttccaccac agaaggactt ccttttgcat cgagaaacga gattaacgcc tcgccggtcc
     8161 ccagttcctg gattgccttt tcggtatcaa acgccggatt ggcccgcatg gtttgcgccg
     8221 cagcttttac cgctttctga tctttgggcg taaaagcccg caaagcgtgt tgaacgcgat
     8281 taccgagctg cccgagcaca ttatccggaa tatcagacgg gttttgcgaa acgaaccaga
     8341 cgcccacgcc ttttgagcga ataagcctta ttacctgctc aatcttatcc agcagtacct
     8401 gcggtgcatc gttaaacagc agatgtgcct cgtcgaagaa aaacaccagt tttggtttct
     8461 ccagatcgcc cgcttccggc aattgttcat acaactctga aagcatccac agcaggctgg
     8521 cggcgtacag tttcggcatc tgataaagct tctcggcgct gaggatattg ataacgcctt
     8581 taccgttggt atcggtgcgc atccagtctt tgatatccag catcggctcg ccaaagaaat
     8641 gcgccgcacc ttgcttttcc agcgacagta aaccgcgctg gatggcacca accgatgcgc
     8701 tactgatatt accgtactga ttctggaaag atttggcgtt atcgccgatg tactgggtaa
     8761 tcgcccgcag atctttaaag tcgagcagca acaatccctg atcgtcagca atacggaaga
     8821 tgatattcag cacgccagat tgcacatcgt tgagattcaa cagccgcgcc agcaacagcg
     8881 gccccaggtc tgaaaccgtc gcccgcaccg gatggccttt ctcgccaaag atatcccaca
     8941 ccaccaccgg attagcatgc ggttgccagt cattgacgcc gatattttta agccttgtga
     9001 gcagtttttc cgacgccgtt cctgcctgcg cgataccggt cagatcgcct ttcacatcag
     9061 ccataaacac cggcacgccg atttccgaca atgactctgc cagtttttgc agcgtaacgg
     9121 ttttacccgt ccccgttgcg ccagtaatca gcccgtgacg gttagccatt cccggcagta
     9181 aaaaaagttc tgtgtccggc gtgcgggcaa ttaatagggg ttcgctcatg agatttcctc
     9241 cagtttcctg cctggagtat aggcaacggc tgcgcactct tcaccgctaa attcagcaga
     9301 ttgcgagacg ccttccaaag agatttaagt gccgggcgac aggcacccgg caaagggatc
     9361 agaagtcggc tttcagaacc acgcggtaac gcgccttacc gtcgcgcaca tgcttgatgg
     9421 catcgttaat tttcgacatc gggaacagtt cggtggtcgg cgcaaccttg ctacgggcgg
     9481 caaagcgcat cagctttcgc agctcataag gcgtgccggt agcagaacca gagatgctgc
     9541 ggtcgcccgc aattaatgta aaggccggaa cagacagcgg cgtgagaacc gcaccgaccg
     9601 tatggaaatt accgccatag gtcagcgcct caaaataagg ctgccagtcg aggctgacgt
     9661 tcacagtatt gataatgaga tcaaactgcc ccgacagggc tttcagtgcc tgcggatcgc
     9721 ggctattcac cactttatcg gcacccatcg ccagtacttc ctgctctttc gccggattag
     9781 aactaaaggc cgtcacctcg catcccattg cgtgcagaag ttttatagcg atatgcccca
     9841 gcccgccaat accaattacc ccaacgcggc tggtagcagt gatatggtgc atcaacagtg
     9901 gtttaaagac cgtgataccg ccgcacaaca gcggcccggc ggactcgata tcaatatttt
     9961 caggcagtgg aatcacccat tgccagtccg cacgcaactt ctcggcaaag ccaccgcgat
    10021 tcataatcgt cggcaccgca ccttgctcgc agttgatctg attaccgcta atacaggcgt
    10081 cgcagtgacc acagctacgc gccgtccagc caatcccgac acgctgaccg acctgcaaac
    10141 ctttatcctg cgcggcgctc ccgagtgcca ccacgcgccc aatcacctca tgcccggcaa
    10201 ccagcggata ttgtgaaaat ccccattcat tgtcgatcat cgatagatcg gaatggcaga
    10261 tcccgcaata atccacctgc acttcaacat cttgtggcct cagctcaccg ggatcgtact
    10321 cataaacttc cagttcgccg cccgcttctt ttgcggcata gctttttatc atcgacattt
    10381 tttggtcctt ctctggtgtt gtttgggaag tgtagagcat ggcagggcgg ggtgcctgga
    10441 gcgtgacaaa ggttacacat cgctgtgtgc aatgctgaaa atttcagcac ttagcgaggt
    10501 gcgagcaagc tggcgcttgc atggtggcgt gcgacaggta taatccacaa cgttttccgc
    10561 atacctcttc agtgccgaag tggcgaaatc ggtagacgca gttgattcaa aatcaaccgt
    10621 agaaatacgt gccggttcga gtccggcctt cggcaccatt cttacttcca agaccatccg
    10681 agaaagtcca aatatccctt taaaatcaat gcttgcagcg attttttcgt cctgcgtctt
    10741 ccgaggttgt ccgttgaaat ccggatatca ttgggggcat aattgggggc atcttaactt
    10801 cgattagaaa tgtgccccca aatgaagctc aacgccagac aggtcgagac cgcaaagcca
    10861 aaagacaaaa cctacaaaat ggccgatggt ggtggtttgt atcttgaggt ttcggccaaa
    10921 ggttccaaat actggcgcat gaaatacaga cgtccctctg acaaaaaaga ggatcgtctc
    10981 gcgtttggtg tttggcctac tgtgacgctt gctcaggcaa gagcaaagcg cgatcaagct
    11041 aaaaagctgt tagcgcaggg cattgatcca aaagccgaac agaaagaagc tcaggccgag
    11101 aattcggggg catattcttt cgaaactatc gctcgtgaat ggcatgctag taataagcgc
    11161 tggagtgaag atcaccgatc acgcgttcta cgttatcttg agctttatat ctttcctcat
    11221 attggttcat ctgacatccg tcagctcaaa actagccacc tattagcccc gattaaaaaa
    11281 gttgatgcca gtggtaagca tgacgttgca cagcgccttc agcagcgtgt cacggctatt
    11341 atgcgttatg cagtacagaa cgattacatc gactcaaatc cagccagtga tatggccggt
    11401 gcgctatcga caaccaaagc gcgacattac cccgctttgc cctccagccg gttccctgag
    11461 tttcttgctc gtcttgctgc atatcgtggc cgtgtaatga cgcggatcgc ggttgagctt
    11521 tccttactaa cttttgtgcg ttccagtgag ttacgtttcg cgcgttggga tgagttcgac
    11581 ttcgataaat ctctctggcg agtacctgca aaacgagaag aaattaaagg tgtgcgttac
    11641 tcgtaccgtg gcatgaagat gaaagaggag catatcgttc agcttagtcg gcaggcgatg
    11701 attttgctaa accagttaaa gcagattagt ggagataaag agctgctttt tccgggtgat
    11761 catgacgcaa ctaaggttat gagcgaaaac acggtaaata gcgcattgcg tgcgatgggc
    11821 tatgacacga aaaccgaagt ttgcgggcac gggtttagaa cgatggcccg tggtgcgttg
    11881 ggcgagtccg gattatggag tgaagatgct atagaacgac agttgagcca ttcagagcgt
    11941 aataatgtac gtgctgctta tattcacacc tctgaacatt tggatgaacg tcgattgatg
    12001 gttcaatggt gggctgatta tctccaagca aataagggca aaacaataac accttatgat
    12061 tttgctaaaa taaacaaaag tcgatagtga aaataaacgc ctataatata taggcgttta
    12121 tttctgtatt aaacactttg atcataatct tccagcagca actttttttc gtacttcatc
    12181 tttccaattg ccactatgga ttattctatc gaaattactt cctgctcttc ctgataataa
    12241 gtcaattcga gatagcttgc tgatatcatc ccagttatca tagaggatat atatagcatt
    12301 accatattct atgttttcaa atacaaccaa atcgtcctcc agcatagctc caatataatt
    12361 actgaacgtg ctgtttcctg tgacgatttt tttaggttca agactcatga agaagtcata
    12421 tctattttca gcgacatttt tcttgtcgga agtgatattt ttacctctaa aaattcttgc
    12481 taaagtttct tctttactac ctggtggaaa tatttcccat gagactataa gtgtatcagc
    12541 ataagtcgat atgggtttat cagcatcctc aattccacat ttaccaagat tttcttgaag
    12601 aagatttaaa tcaaataata attcatcatc aaacttattt gattgtttgt ctaaaattct
    12661 agttactctg aagttaaata tgaaatggct gtccgagggc tgtttgtgat taatttcaat
    12721 ggctatgagt gttggtggta taatgtctct gggatatttc tcgtaaggta atctaacagt
    12781 atgtgtacca ttagagctat caccccagtt aggtgactca atttcagtgt aatgagtttc
    12841 ttttgggagg tcttttctga caatttcttc accaaaaaca tttttaagtg aatatttacc
    12901 ctgcaatgga tctggtatga ccgacgtctc ataagcatta acaccattat caaaatatat
    12961 tccaaggtgc tctaatgctc cactttttaa ttggttttcc gtaaatgcaa tagaagtgcc
    13021 agcaacaact gtattaccaa gccttcttaa cttgctatga atatttttag gtataacacg
    13081 tatattcttt ttcatttttc cctcatactt gatcatcaat gtctctttgc tctcgcttgt
    13141 tatgtaaatc attgataaac ataacatttg caagtattac gctaattact gcctcaatct
    13201 gcatgtacga aatagcaata attaatgcta aagatgtgtt ttttattaca tcttggctct
    13261 ctaaataaag atgacttttg aaaaacacta gtttaactaa gtatacaaca agtaaagtaa
    13321 tcataacgaa agatgatttt agaacacttc caactattgc ttctaatctt cttgtatctt
    13381 ccagttgttc ggtgaaatca acggtttcta tagttttggg gttcacaata cgctgcaaag
    13441 catttgggta aagaaacgct aaccaaattc ccataatagt aaaaaccatt ccagaaactg
    13501 atacgagaag agtgcaatag ttggcaaaat cgttatacac atagttaata tcggatgacg
    13561 aaataatcat cagcgatata atgaatatcg ccatgtatat ccatgtgtat ttggctatgc
    13621 ctgctatagc tttcaatatt caatcctctc cagatgcttt ctttattctt ggttgagatt
    13681 gtaatgcttt taattctttt aatatagggg aaataaattc ttttcttttg tgtgatattt
    13741 gttcataaat atatgcggcg gaatatgttg aatagtcgtc agaagggaca ttgagaatat
    13801 cacgcattct atatttatcc acccaagtta tacccttgtc tgtagcaaat ccaacattgt
    13861 cccaaagacg tttttctcta tcctcagccg aatacttttc aatgatttct tgaacttcat
    13921 tcaaggatgg ttttgcttct gcttttattt caatttgtct tgttttggca tttttctttc
    13981 cggacacaaa tggaacgaga gtgctaaagg tctttaacca atcagctcgt gcatcatttg
    14041 gatttataat aatagtttcc ctgcgaacta tgtgtgttat ttttggtatt agtttaccta
    14101 actcagtatg agtggtttct aagcttctga gcttcatatc aaacctgtac atatatttat
    14161 ataagtcttc gtcatcggta ttagatagcc ttatataacc tttctcggtt ttatttttaa
    14221 cccttttgct atgtttaact ctattagtaa tagatgagtg tacatagtct tgaaatagtt
    14281 cagaatcaca aacagaatgg tcgaatttaa tggatactat agtttcaagt tcaggtataa
    14341 cccaataaaa gcatggccgc ccccagataa cttttttccc tctgtagcta ttagtatatt
    14401 taaccaccga tgagcttccg atttcaccgt cttcactagc tcctaataaa gagcccgtac
    14461 tatcagtatc agatttccat aacatgacta agaaatcacc agtattttca tccttataaa
    14521 tatccttgca ataacacttt gaactgtttg gtttttcttt tggatcccat ggtatcgtga
    14581 gtgcaagaga tttagttccc acccaatcct gtatgaggtc aaatgtttcg ctaagttcta
    14641 aaccataggt attgtcatca tttaccttgt ataaaccgca cttattaacc ttgaagaaat
    14701 tgagatgccc tacattcatg ctctttcctt agcttcttat cttgggatat gtatcatgat
    14761 caaaaattaa gcataacccc attgacttcg tgcgggcaat gttttcaccc tagaatttga
    14821 tcggtttaac gttaaatatt cataagttac atgagcattt ttcacttctt cccaattaca
    14881 gacgtcatga gctgaatatt aataaaataa ttatcctcac ggcatggcgc gcaatgctct
    14941 ccccgccacg cccgcccgct ttgcagggcg gttttaatgc agttgcacag tcacgctcag
    15001 gctgcgccgg gaatggcgcg gtctgcagaa aatgaggcag gaaaacgcat gcaaagccat
    15061 gcaccttatc gatgcatggc ttttttcagt aaaaacgggc ggattttcgg ggaattttac
    15121 acagactgac gtgatgccag ttgcgcactt ttacgcgaaa aaatcatgtt ctgcgcaggg
    15181 gtgaattttt cacggctgtc atccaccgaa gtcgcgtcag gcctgaatcc gatggtcgtt
    15241 aaaatgtcac tatcctgtgc ggaataatta attttttccc ccttcgcaag ccaggacaga
    15301 agggcttcac gcagggcatc tgtggcacgc tgtatggcac agtttcgggc aatggccgtc
    15361 agctcactgt agcccatcag ctccggtgcc agtgccgccg ccagtgctgt gccgtgctgc
    15421 tgcataaaat cattcagccg gtcgcggatg ctgatgtgct gaacggcttc atgcgaacga
    15481 atataacgac cggcagcctg attcacctgc cattttctga cttcgataat attgcgtaat
    15541 tcgtccaggc gactgacgtt tctgccttcc ccggacagaa gccgcagata ttcctgttcg
    15601 gccgcagcca gctcattttt gcgttgcagc catgctgctt tgttattctg acaggtgtca
    15661 aaggcctgct gtaaggctgt gctttccatc gttatctctt tctcatcatg ctgaagaata
    15721 aaaatacggt gtgcggcgac ggccggtgtt aaccggcagc cctcattcca gacgcagcga
    15781 atatgattgt gtttttaagc gtactggcgg cagttcctgt ttttcatgca ggctttctgc
    15841 cagttcgtcc ggcgtgaccg ggcggacaat aaaacggttg atggtctgaa gcgttttaaa
    15901 caccagacca cagcccggat ccgtgcacac ataaaaacgc tcggtgactt cctgagacag
    15961 acgccgcgat gttcttgaca gtgcaaggcc tttacatctc cgacaacaat atccggtaac
    16021 aagcattctt ttcgggcgtt tcatactgcc ggaggctgac gtcagtgaat cgcggtatct
    16081 ctgtttgcct gaaatgtatt ccattcctga atctttacag tcagagaaaa agctttcact
    16141 cgcttcaaat gtcgcagagc aataaatatt ccggcactgt gcaatcatta tcttggtgcc
    16201 atcgtccatg aaatgtgcgc gacgggtgtg agcaacatgt ccacacgacg ggcagtaaat
    16261 catgacagca gtcctctggc cttaagctct gctccctgct ggtctatttt gtcctgccac
    16321 accttgcgct gtgccggtgt gcctgccacc tcataatcca tgtgcgggag tgttgccgct
    16381 gacagtccgg tcagccggag aaccggctcg ccggtgaggc tgatttgcat ctgtttaatt
    16441 ttctgttcca gcgatgattt cacctgctgc atgacagcct tttccggtgc gacgtagccc
    16501 tgatggccgg tggtgttggc gagcggattt tcctgtacca ggatactcag atgcattgcc
    16561 cggacaagcg cctcacaggt ttcattcagg gcgtgttcca gctcatgctc tgcatacaga
    16621 ctcagcaggt gatgatgtgc cttccggtag gcggtggccg tgctgtcaca cgcccctttc
    16681 aggcgttcac gttcaaaatt cagcaccacg gccagattgt catattcctg taccagctcc
    16741 cggcgtgcca cgcgctcaat gtggcgctgt ttcagctcgt cgctcaggac accaccggct
    16801 gcacgaaagg ccgtgcgcca gtcgtcagcg tcatttccgt cggcctgcgc cagcgcattt
    16861 ttttcctgct ctgcccgttc aatggccgtg acggtctcat ccatcaggcg ggcgttctca
    16921 aggtgggcgg ctctggcctt ttccagttgt gccagtgcgg gttgcagata ttcagggatg
    16981 gtgttgtcag acattttccg gctcctcgtc acttcaggtt aagaaaattg tgacgtacac
    17041 cggacaacaa cacgacgcat tgcagatgtg ccagccctga cacaggagac tcatcctcag
    17101 accggcaagc caggaaaagg tcgcaggaaa aaccggctta ctgtttgttt ttttatattt
    17161 tactgttcac ctctgttcac cataagaaaa aagataagta atacagtaag ttaaagggtg
    17221 aacaatcgca gtaatgactg ttcaccgtct gttcaccact gttcacccgc tcatagactt
    17281 tttgtgctgt ttattacttt ttatttttat taatccactg aaataaataa gaaaaaacaa
    17341 tttgtatttc actataattt tttccagctc cttccagagc gttttaaagc catctggcgc
    17401 tgatgtataa aaaatacaca gccattgtaa gactgccgga acaaatcccc cctgttgcat
    17461 ctgccaaaaa tattcacaaa ataaagcgct acccgaagcc ggacggactt atccggtgct
    17521 gtatggacat taacgaggta gcccgatgca agctgttttt tcttccccgt ctcccgcccc
    17581 tgtgacgcca ctgatgccgc tgccggacat cacgcaggag cgttttttac gtctgccgga
    17641 agtgatgcac ctgtgcggcc tgtcacgctc gaccatctat gaactcatcc gtaaggggga
    17701 atttccgccg caggtgagtc ttggcggtaa aaatgtggcc tggctgcact ctgaagtcac
    17761 cgcatggatg gccggacgca ttgccggacg caaacggggg tacgacgcat gatgatgccc
    17821 gctctgcaaa aactcccttt ttctggcttg cctttttccg gcatttgcgg atatagtttt
    17881 tccgctgccg caaaatcggc agccgggcgt gagaacccga gcaattcaca ggcgacatat
    17941 gacgcgccac gcgtcttttt ttgtgtcgca atcaatgcca cagagcgcca gattatggtg
    18001 tggtgtgtgg tcagtcgtgc agatggcatt ccgtgcatcc tgcccgcgtc cgctcattat
    18061 gccgcagagt caatggtagc tcaggcgggg cagcctccgg gctggccggt atcctgtgaa
    18121 gccggtattc tcacccccgt ctgggctatc gccatcgagc gtgagaactc cggcgatagc
    18181 gttatttgct attcacagga ggctgccatc atggctacaa cccttacccc ctcacaccct
    18241 gaatttgtct ttgtgtttgc ggctgtccgt cgcgcagacc gtcatccccg tatctgtatg
    18301 cttcgcaccg tggccggtga tgaacgcagt gcccgccgtt cccttgtccg tgactatgtg
    18361 ctctcccttg ctgcccgtct gccggtggtg gaggtgtccc gtgcgtaata aaaaagcccc
    18421 tcagaccgtc tcagcacgtc atgacgcccg tgaacacctc agcattgagg cttaccataa
    18481 gctcaaccgc gccagcgccg tatcccagtt tgttgggggt gatttgattc accgtgaact
    18541 ctccggcctg catcagctct acattccgca tattttcagc tacctgaatg aagatattga
    18601 ttttgtgctg aatgagctga aagccaaagg cctgtgccgc gattttctcg cccagcagaa
    18661 agaccgggga gacaggacgc atgtttgatt ttccccagcc cggtgagatt taccgttctg
    18721 ccggttttcc cgatgtggcc gtggtcggca ttctggaaga cggtattccg tgggaaatgc
    18781 cgtaccgctg cccggacatt gtctggaacc cgtaccgccg taaattcagt atccttgtgc
    18841 gtatcctcgc cgacgggcgc accacagaca tcccgctggg gcgttttctg cgggaattta
    18901 cctgtgaccg tcctgacctg tttaaacgca gccccgtaaa ccggcatgcg gtactgaaag
    18961 aaatggccgg agacccggaa ttacagaaat ggcgggagaa atatcaggat atttacccgc
    19021 aggacactgt tccggtcagc cgggcagcac cggtagcgcg ggaatggcgg gaaattcccc
    19081 gcacggagcc tgacccggaa atcaccccgg ataacagtta ccgcaattat ctgtaattaa
    19141 aaaacgacac ccgaaaaatt aaatgtgcgt attcgcgcag ggatacgcac gtcttcagga
    19201 gacgcagata tgccttatca gttaatgcaa ccggcacgga atgcagtcat ctgtcacagg
    19261 gaggaaagca aatgaaaaca cccttaccgc ccgtcttacg cgcagccctt taccgtcgtg
    19321 ctgtcgcctg tgcctggctg accgtgtgcg aacgtcagca ccgctacccg catctcaccc
    19381 ttgagtcact ggaggcggcc atcgccgctg agctggaagg cttttatctg cgccagcacg
    19441 gtgaggaaaa agggcgtcag atagcctgtg ccctgctgga agatttaatg gaatccggcc
    19501 ccctgaaggc cgcaccgtcg ctgtcctttc tcgggctggt tgtgatggat gaactctgtg
    19561 cccgtcacat aaaagcgccg gtactgcact gaaggagaac aacaccatga aaatgaacgt
    19621 aaccgccacc gtcagccatg cgctcggcca ctggccgcgt attctcccgg cgctggggat
    19681 tcaggtgctg aagaaccatc atcagccctg tccggtctgt ggcgggagtg accgcttccg
    19741 ttttgatgac agggaggggc gcggcacctg gtactgcaat cagtgtggtg ccggtgacgg
    19801 cctgaaactg gttgaaaagg tgtttggtgt ctccccgtcc gacgtggccg caaaggtggc
    19861 tgccgtgacc ggcagtctgc ccccggctga cccggcagtg acggccgccg ccgttgctga
    19921 aacagacgct gcccggaaga acgccgccgc actggcacaa accctgatgg caaaaacccg
    19981 tcccggaacc ggtaacgcct acctgacccg caagggcttt cccggccggg aatgccggat
    20041 gctgaccggc acacacagag ccggtggcgt gagctggcgc gccggtgacc ttgtggtgcc
    20101 actgtatgac gacagcggcg aactggttaa ccttcagtta atcagtgctg acggccgtaa
    20161 gcgcaccctg aaaggcggac aggtcagggg cacctgtcac acccttgaag gacagaatca
    20221 ggccggaaaa cgtctgtgga tagcggaggg atacgcgacc gcacttaccg tacatcacct
    20281 gaccggtgaa acggtgatgg tggcgctttc ttccgtgaac ctcctttctc tggccagcct
    20341 tgcccggcag aagcatccgg cctgtcagat tgtccttgcc gcagaccgtg acctcagcgg
    20401 tgacggccag aaaaaagccg ccgcagccgc agatgcgtgt gaaggtgttg ttgccctgcc
    20461 gccggtcttc ggtgactgga atgatgcctt cacgcagtac ggcggggagg tcacccgtaa
    20521 ggccatttac gatgccatcc ggccaccggc tgaaagcccg ttcgacacca tgagcgaagc
    20581 agagttttcc gccatgagta ccagcgaaaa ggccatgcgt atctatgagc attacggcga
    20641 ggcgctcgcg gtcgatgcca acggccagct tctgtcccgc tatgaaaatg gtgtctggaa
    20701 ggtgctgccg ccactggact ttgcccggga tgtggccggg ctgtttcagc gtctgcgcgc
    20761 gccgttctcc tccgggaagg tggcctccgt ggtggacacc ctgaagctga ttattccgca
    20821 gcaggaagcc ccctcccgcc gcctgattgg ctttcgtaac ggtgtgctcg acacacagaa
    20881 cggcacgttc cacccgcaca gtccgtcaca ctggatgcgt accctgtgcg atgtggattt
    20941 caccccaccg gtggaagggg aaacgctgga aacccacgcc cccgcgttct ggcgctggct
    21001 tgaccgtgcc gccggtggcc gtgcggaaaa acgcgacgtg attctggccg cactgtttat
    21061 ggtgctggca aaccgctacg actggcagct ctttctggag gtgaccggtc ccggcggcag
    21121 cggcaaaagt atcatggccg aaatcgccac cctgctggcc ggggaggata acgccacgtc
    21181 ggccaccatt gagacgctgg aatccccgcg tgaacgtgcc gcgttaactg gcttctcact
    21241 gatacgcctg ccggaccagg aaaaatggag cggcgacggt gccggactca aggccatcac
    21301 cggcggcgat gcggtgtccg tggacccgaa ataccgggat gcgtactcca cgcatatccc
    21361 ggcggtgatt ctggccgtga acaataaccc gatgcgcttc accgaccgca gcggcggcgt
    21421 gtcacgccgg cgggtgatta ttcacttccc ggaacagata gccccgcagg agcgcgaccc
    21481 gcagcttaag gacaaaatca cccgcgagct ggcggtcatc gtgcgtcacc tgatgcagaa
    21541 gttcagcgac ccgatgctcg cccggtcact gcttcagtcc cagcagaact cagacgaggc
    21601 gctgaacatc aaacgggatg ccgacccgac gtttgatttt atcggctatc tggaaaccct
    21661 gccgcagacc agcggcatgt atatggggaa cgccagtatc atcccgcgta attaccgtaa
    21721 atacctctat cacgcctatc tggcctacat ggaggcaaac ggctaccgga atgtactcag
    21781 tctgaaaatg ttcgggctgg ggctgccggt gatgctgaag gaatacggac tgaattacga
    21841 gaagcgccat accaaacagg ggatacagac caacctgaca ctgaaagagg aaagctacgg
    21901 cgactggctg ccaaaatgtg acgaccctgc aacaacctga cccacctgac cggcatctgc
    21961 cggtcttttt ttatcccgac ctcccccgaa ggtgaacaat ccactgttca cccttcaccg
    22021 tatattcacc cgttatcaca ctgaaattaa aagagaaaaa tgaaaggtga acagtgtgaa
    22081 cagttctttc gaaaaaaaat tttttatgtg gataaaagat gcactggttt ggatccgggc
    22141 tacggatcca aacttactga ttaaaaatta tttaactttc cgatttattg atgggctgaa
    22201 aatagattgg tcagaaaaac agtgggggca caaaaggggg catatttcat tgttgtgttt
    22261 tattatttgt tttttatcat taacttattt tgatatttga gtccggcctt cgcaccaaaa
    22321 gtatgtaaat agacctcaac tgaggtcttt ttttatgcct gaaatccgcg ctacacaagg
    22381 cttttccagc ttttcccctc aactgaagtc aacctgactc aacccacatc aacaggcttg
    22441 tgagtatact aatgagtata tttgccgatt cgatattgct tgtatactca cgagatacca
    22501 atggaaggaa gaccattatg gccctaacgg atactaaagt tcgctctgcg aaacctgagg
    22561 agaaagagta ttcacttgtt gacggcgacg gcatgtcctt acttgtaaaa cctggcggtt
    22621 caaagtattg gcgatttcgt ttccgttttg gcggtaaaca acatttgatg gcgttcggcg
    22681 tttacccgga tgtttcgctg gcggatgcgc ggaagaaaag ggaagaggcc agaaagctgg
    22741 tagctgctgg tatcgatcct cgtgagcata agcgtgctgt gaaagaagag caggcgaaag
    22801 agattattac tttcgagaag gttgccagag agtggctcgt aaccaaccaa aaatggtcgg
    22861 aagagcatgc taatcgtgta aaaaagagcc tggaggacaa tatcttcccg gcaattggtt
    22921 cccacaatat tgctgaactg ggcacccgcg atctgttaat tcctattaaa gccgtggaga
    22981 aatccggacg tcttgaagtg gcttcccgcc ttcaacagcg caccacggcc atcatgcgtt
    23041 atgcagtgca aagcgggtta attgattaca atccggcgca agagatggcg ggggcggtag
    23101 cttccagtaa tcgacagcat cgtccggcgc tggagttaaa gcgtatcccg gaattactcc
    23161 agaaaatcga tgaatacacc ggcaggccac taacccgctg ggcaaccgaa cttaccctgc
    23221 ttatttttat tcgatccagt gagctgcgtt ttgctcgctg gtcagaaatc gattttgaaa
    23281 cgtcaatgtg gaccatcccg cctgagcgag aacctattcc cggtgtgaaa cattctcagc
    23341 gaggagcaaa gatgcgtaca cctcatctgg tgccgctttc aaagcaggcg ctagcgatcc
    23401 tgaagcagat aaaacagttc tgtggcgaac atgagctgat ttttattggc gatcacgatc
    23461 cgagtaagcc catgagtgaa aacacggtga acagtgcatt gcgggtgatg ggctatgaca
    23521 ccaaggttga ggtttgcggc cacggcttcc gcactatggc ctgtagctca ttgattgagt
    23581 cagggttatg gtcgaaggat gcggtggaaa ggcagatgag ccatatggaa cgcaattcgg
    23641 tgagggcggc gtatatccat aaagcggaac atctggatga gcgcaagctg atgttgcagt
    23701 ggtgggctga ttttctggat gcgaaccggg agaaggggat tacgccgttt gactatgcga
    23761 agatcaatcg gggtaatggc gagtgagtta tctaaaagcg cacattcgcc tctaaattga
    23821 aagctgctga cttattcaaa cgaggaattg gtcagcagtg ccaaatagcc gagttttcac
    23881 taaggtaagc ctttttattg aacctaatcg gcagtaatgc tccacctaag cctaaattta
    23941 aagtcatgat tacttataaa aggggagtgg acccttgatt gagccctaca tcgtaacaaa
    24001 ggtacagcaa ttcggctaca tcaggagtga tagtcaatta gttaatctaa taattaattg
    24061 aatctaaata attaaatttc ttacgtaaaa ttgaccaatt tactggtggt gtaagccgca
    24121 atatcttaat gtaatcagta taaaggggct tgatttcttt taaagatagt atccgtatga
    24181 attttaagaa aaaactcgaa gagcatttta agcagttcga agcatcccca gtgttatttg
    24241 tcggctccgg tgtgtctcgt cgttatcttg gtgtgccatg ttggcaagac cttttgaagc
    24301 attttgctga ggcaattggg gaaaatcata taaaattaaa aacaaaatct aatggtgact
    24361 tacccgaata tgcacaatta ttggtgtctg catatgctga gaaatggtgg gatactgaag
    24421 aaggacaatt agcattaagt gaaaaagaac aggagaaaac tttcattaac gaacagtctc
    24481 ctttgaagtt gtcaatatct aaatatattg agaatgcaca taaaaatatt atagataatg
    24541 atgagttaaa acatga
//
LOCUS       DAJTMC010000050        24548 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.050, whole genome shotgun sequence.
ACCESSION   DAJTMC010000050 DAJTMC010000000
VERSION     DAJTMC010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24548
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.050"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(<1..146)
                     /locus_tag="OO130_001772"
     CDS             complement(<1..146)
                     /locus_tag="OO130_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HCR5365219.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TNDKR"
     gene            complement(405..1286)
                     /gene="yddG"
                     /locus_tag="OO130_001773"
     CDS             complement(405..1286)
                     /gene="yddG"
                     /locus_tag="OO130_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415990.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HCR5365220.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEAGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGASLWVYYFLTQQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
     gene            1518..4565
                     /gene="fdnG"
                     /locus_tag="OO130_001774"
     CDS             1518..4565
                     /gene="fdnG"
                     /locus_tag="OO130_001774"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:2103..2105,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCR5365221.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIAVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLAGQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGINNGERVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            4578..5462
                     /gene="fdnH"
                     /locus_tag="OO130_001775"
     CDS             4578..5462
                     /gene="fdnH"
                     /locus_tag="OO130_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HCR5365222.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEAPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            5455..6108
                     /gene="fdnI"
                     /locus_tag="OO130_001776"
     CDS             5455..6108
                     /gene="fdnI"
                     /locus_tag="OO130_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HCR5365223.1"
                     /translation="MSKSKMIVRTKFIDRVCHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(6159..6443)
                     /locus_tag="OO130_001777"
     CDS             complement(6159..6443)
                     /locus_tag="OO130_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCR5365224.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVTQ"
     gene            complement(6589..7599)
                     /gene="adhP"
                     /locus_tag="OO130_001778"
                     /pseudo
     CDS             complement(6589..7599)
                     /gene="adhP"
                     /locus_tag="OO130_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415995.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
     gene            complement(7733..9430)
                     /gene="maeA"
                     /locus_tag="OO130_001779"
     CDS             complement(7733..9430)
                     /gene="maeA"
                     /locus_tag="OO130_001779"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCR5365225.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     IDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            complement(9587..9724)
                     /gene="sra"
                     /locus_tag="OO130_001780"
     CDS             complement(9587..9724)
                     /gene="sra"
                     /locus_tag="OO130_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HCR5365226.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            complement(9826..10041)
                     /gene="bdm"
                     /locus_tag="OO130_001781"
     CDS             complement(9826..10041)
                     /gene="bdm"
                     /locus_tag="OO130_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HCR5365227.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAEHGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            10387..10818
                     /gene="osmC"
                     /locus_tag="OO130_001782"
     CDS             10387..10818
                     /gene="osmC"
                     /locus_tag="OO130_001782"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HCR5365228.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSFMLGEAGFTPTSIDTIADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
     gene            complement(10874..11800)
                     /locus_tag="OO130_001783"
     CDS             complement(10874..11800)
                     /locus_tag="OO130_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5365229.1"
                     /translation="MSDTLLTLRGVHINFPARKNWLGKTTEHVHAINGIDLQIRRGET
                     LGIVGESGCGKSTLAQLLMGMLQPSHGQYIRSGSQRIMQMVFQDPLSSLNPRLPVWRI
                     ITEPLWIAKRSSEQQRRALVEELAVQVGIRPEYLDRLPHAFSGGQRQRIAIARALSSQ
                     PDVIVLDEPTSALDISVQAQILNLLVTLQENHGLTYVLISHNVSVIRHMSDRVAVMYL
                     GQIVELGDAQQVLTAPAHPYTRLLLDSLPAIDKPLEEEWALRKTDLPGNRTLPQGCFF
                     YERCPLATSGCEVRQSLAIREDGRELRCWRAL"
     gene            complement(11793..12779)
                     /locus_tag="OO130_001784"
     CDS             complement(11793..12779)
                     /locus_tag="OO130_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5365230.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGDDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIVLLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGVRADVIHHPRHPYTIGLLQCAPEHGVPRQLLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPALTACGDNNQRCACWYPQQEVISV"
     gene            complement(12776..13672)
                     /locus_tag="OO130_001785"
     CDS             complement(12776..13672)
                     /locus_tag="OO130_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HCR5365231.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLML
                     LMMFFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLISWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPRAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            complement(13669..14691)
                     /locus_tag="OO130_001786"
     CDS             complement(13669..14691)
                     /locus_tag="OO130_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5365232.1"
                     /translation="MTFWSILRQRCWGLVLVMAGVCVITFIISHLIPGDPARLLAGDR
                     ASDAIVENIRQQLGLDQPLYVQFYRYVSDLFHGDLGTSIRTGRPVLEELRIFFPATLE
                     LAFCALLLALLIGIPLGILSAVWRNRWLDHLVRIMAITGISTPAFWLGLGVIVLFYGH
                     LQILPGGGRLDDWLDPPTHVTGFYLLDALLEGNGEVFFNALQHLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDYIRTARASGLPGWYIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTETVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGGE"
     gene            complement(14693..16243)
                     /locus_tag="OO130_001787"
     CDS             complement(14693..16243)
                     /locus_tag="OO130_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR5365233.1"
                     /translation="MKRSIPFRPTLLALVLATTFPVAHAAVPKDMLVIGKAADPQTLA
                     PAVTIDNNDWTVTYPSYQRLVQYKTDGDKGSTDVEGDLASSWKASDDQKEWTFTLKDN
                     AKFADGTPVTAEAVKLSFERLLKIGQGPAEAFPKDLKIDAPDEHTVKFTLSQPFAPFL
                     YTLANDGASIINPAVLKEHAADDARGFLAQNTAGSGPFMLKSWQKGQQLVLVPNPHYP
                     GNKPNFKRVSVKIIGESASRRLQLSRGDIDIADALPVDQLNALKQENKVNVAEYPSLR
                     VTYLYLNNSKAPLNQADLRRAISWSTDYQGMVNGILSGNGKQMRGPIPEGMWGYDATA
                     MQYNHDETKAKAEWDKVTSKPTSLTFLYSDKDPNWEPIALATQSSLNKLGINVKLEKL
                     ANATMRDRVGKGDYDIAIGNWSPDFADPYMFMNYWFESDKKGLPGNRSFYENSEVDKL
                     LRNALATTDQTQRTRDYQQAQKIVIDDAAYVYLFQKNYQLAMNKEVKGFVFNPMLEQV
                     FNINTMSK"
     gene            complement(16257..16838)
                     /gene="ddpX"
                     /locus_tag="OO130_001788"
     CDS             complement(16257..16838)
                     /gene="ddpX"
                     /locus_tag="OO130_001788"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HCR5365234.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRPQQAQAMLWQACPDPQYVVDVTVGSNHSRGT
                     AIDLTLRDEHGNILDMGAGFDEMHERSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGIS
                     SEWWHFELPQAASYPLLADQFTCFISPGTQHVS"
     gene            complement(17096..19495)
                     /gene="dosP"
                     /locus_tag="OO130_001789"
                     /gene_synonym="pdeO"
     CDS             complement(17096..19495)
                     /gene="dosP"
                     /locus_tag="OO130_001789"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase"
                     /protein_id="HCR5365235.1"
                     /translation="MKLTDAENAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKL
                     WGYKREEVIGNNIDMLIPRDLRPAHPEYIRHNREGGKARVEGMSRELQLEKKDGSKIW
                     TRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
                     CNRAFTEMFGYCISEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRT
                     GEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGE
                     IICRNIESVLNESHVSLFALRNGMPIHWASSSHGAEVQNAQSWSATIRQRDGAPAGIL
                     QIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTDLPNRNNLHN
                     YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFRGKLKPDQYLCRI
                     EGTQFVLVSLENDVSNITQIADELRNVVSKPIMIDDKPFPLTLSIGISYDVGKNHDYL
                     LSTAHNAIDYIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAISNNQLKLVYQPQIF
                     AETGELYGIEALARWHDPQHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRS
                     QNIHIPALSVNLSALHFRSNQLPNQVSDAMHAWGIDGHQLTVEITESMMMEHDTEIFK
                     RIQILRDMGVGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKRILALLEAI
                     TSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMSSVLPLK
                     I"
     gene            complement(19520..20901)
                     /gene="dosC"
                     /locus_tag="OO130_001790"
                     /pseudo
     CDS             complement(19520..20901)
                     /gene="dosC"
                     /locus_tag="OO130_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
     gene            complement(21273..22592)
                     /locus_tag="OO130_001791"
     CDS             complement(21273..22592)
                     /locus_tag="OO130_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 10 protein"
                     /protein_id="HCR5365236.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIT
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            complement(22723..24258)
                     /gene="gadC"
                     /locus_tag="OO130_001792"
     CDS             complement(22723..24258)
                     /gene="gadC"
                     /locus_tag="OO130_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HCR5365237.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
BASE COUNT         6127 a         6186 c         6190 g         6045 t
ORIGIN      
        1 cgcttatcat tagtaaaata acgtagagcg gtaaccttgc cataaaggtc gagtttatta
       61 ccatccttgt tatagacttc ggcagcgtgt gctacgttcg cagctaacaa accagttaca
      121 accaccgcaa ctatttttaa tttcatttaa cagaatcctt ttaattatcg tcaaacgtat
      181 tttctaaacg aattttaaac ggcgtcattt ataaatgaca tactgttttt aaaatagaat
      241 ggtgattaaa aacaatgaga aaaatagatg agataatatt atttatcgat atgtgatcga
      301 agtcgaaatg agatataagg tgaattactg gtatttgaaa tttatttttt aatattgtcg
      361 gaatttatct gattaactgc cgggccgtag acccggcaat tattttaacc acgacgtgtc
      421 gccagccagc agagcaggga accgccgcag accattagcg cgccttgcca gaatgagaac
      481 gacagcgggg cgctgagcag cacggctgca agcgctgagg aaagtacggg cgtaaaatac
      541 gagcctaccg ccattatggt gacattgcca tgcaatatac cgacattcca tgcagcataa
      601 gcaaatccta aggtaaatgc cgcagagatg agtttaatca tgacgggcgt gctaaatacc
      661 atttctggtt gttgcgtaag aaaatagtaa acccacagac ttgctcccgt tagcaggaca
      721 aaaacggtaa ttccgttaaa tccgcgtgcg tatttattcg ttactgtgca ataggctgcc
      781 cagataaacg caccaataaa cgccaggaaa taactcaatg ggctggtggt gatattattg
      841 atgatttcat cataatgtaa cccattctca ccgcctaaca cccagcagac gccgaccagg
      901 gctaataata atcctggtac aatcagccag ttggttttct gaccattaaa cagaatggca
      961 aagagaattg tcaggctggg ccacagatag ttcaccatac ccgcttcaat cgcctgatga
     1021 cgggtcgccg cataccctaa ggaaagcgcc agacagattt catagctgac gaataacaga
     1081 ctcccggcga gtaaatagcc tttcggaatc tgccgaatac gcggaaatcc aaccgtgaag
     1141 attaacagca gcccgcttaa tgaatagata gcagctgcgc cgccgaccgg gccgagcccc
     1201 tcgctgacac cgcgaatcaa tcctaccatc gtgctccaca ggacgatcgc tattagccct
     1261 atgagcgttg ctttttgtcg tgtcatgctc gctgttttgt ctctcttgcc gttaaaaatt
     1321 aagctgaatt ttatagcatt tttttaactg gcctgtcagg cagtggtgcg tttttctacc
     1381 gctattgagg taggtcaatt tgcgcaggcg gattattttg tggcaaacac gtgttctttt
     1441 tgatttcgcg caaaaagatt cagaatttta ctgttagttt cctcgcgcag taatatccct
     1501 gaaaaaagag gaaagcaatg gacgtcagtc gcagacaatt ttttaaaatc tgcgcgggcg
     1561 gtatggctgg aacaacggta gcggcattgg gctttgcccc taagcaagca ctggctcagg
     1621 cgcgaaacta caaattatta cgcgctaaag agatccgtaa cacctgcaca tactgttccg
     1681 taggttgcgg gctattgatg tatagcctgg gtgatggcgc gaaaaacgcc agagaagcga
     1741 tttatcacat tgaaggtgac ccggatcatc cggtaagccg tggtgcgctg tgcccaaaag
     1801 gggccggttt gctggattac gtcaacagcg aaaaccgtct gcgctacccg gaatatcgtg
     1861 cgccaggttc tgacaaatgg cagcgcatta gctgggaaga agcattctcc cgtattgcaa
     1921 agctgatgaa agctgaccgt gacgctaact ttattgaaaa gaacgagcag ggcgtaacgg
     1981 taaaccgttg gctttctacc ggtatgctgt gtgcctccgg tgccagcaac gaaaccggga
     2041 tgctgacaca gaaatttgcc cgctccctcg ggatgctggc ggtagacaac caggcgcgcg
     2101 tctgacacgg accaacggta gcaagtcttg ctccaacatt tggtcgcggt gcgatgacca
     2161 accactgggt ggatatcaaa aacgctaacg tcgtaatggt aatgggcggt aacgctgctg
     2221 aagcgcatcc cgtcggtttc cgctgggcga tggaagcgaa aaacaacaac gatgcaacct
     2281 tgatcgctgt cgatcctcgt tttacgcgta ccgcttctgt ggcggatatt tacgcaccta
     2341 ttcgttccgg tacggacatt acgttcctgt ctggcgtttt gcgctacctg atcgaaaaca
     2401 acaaaatcaa cgccgaatac gttaaacatt acaccaacgc cagcctgctg gtgcgtgatg
     2461 attttgcttt cgaagacggc ctgttcagcg gctacgacgc tgaaaaacgc cagtatgata
     2521 agtcgtcctg gaactatcag ttcgatgaaa acggctatgc aaaacgcgat gaaacactga
     2581 ctcatccgcg ctgtgtgtgg aacctgctga aagcgcacgt ttcccgctac acgccggacg
     2641 tcgttgaaaa catctgcggt acgccaaaag ccgacttcct gaaagtgtgt gaagtgctgg
     2701 cctccaccag cgcaccggat cgcacaacca ccttcctgta cgcgctgggc tggacgcagc
     2761 acaccgtggg tgcgcagaac atccgtacta tggcgatgat ccagttgctg ctcggtaaca
     2821 tgggtatggc cggtggcggc gtgaacgcat tgcgtggtca ctccaacatt caggggctga
     2881 ctgacttagg tctgctctct accagcctgc caggttatct gacgcttccg tcagaaaaac
     2941 aggttgattt gcagtcgtat ctggaagcga acacgccgaa agcgacgctg gctggtcagg
     3001 tgaactactg gagcaactat ccgaagttct tcgttagcct gatgaaatct ttctatggcg
     3061 atgccgcgca gaaagagaac aactggggct atgactggct gccgaagtgg gaccagacct
     3121 acgacgtcat caagtatttc aacatgatgg acgaaggcaa agtcaccggt tatttctgcc
     3181 agggctttaa cccggttgcg tccttcccgg acaaaaacaa agtggtgagc tgcctgagca
     3241 agctgaagta catggtggtt atcgatccgc tggtgactga aacctctacc ttctggcaga
     3301 accacggcga gtcgaacgat gtcgatccgg cgtctattca gactgaagta ttccgtctgc
     3361 cttcgacctg ctttgccgaa gaagatggtt ctatcgctaa ctccggtcgc tggttgcagt
     3421 ggcactggaa aggtcaggac gcgccgggcg aagcgcgtaa cgacggcgaa attctggcgg
     3481 gtatctacca tcatctgcgc gagctgtacc aggccgaagg tggtaaaggc gtagaaccgc
     3541 tgatgaagat gagctggaac tacaagcagc cgcacgaacc gcaatctgac gaagtggcta
     3601 aagagaacaa cggctacgcg ctggaagatc tctatgacgc taatggcgtg cttattgcga
     3661 agaaaggtca gttgctgagt agctttgcgc atctgcgtga tgacggtaca accgcatctt
     3721 cttgctggat ctacaccggt agctggacag agcagggcaa ccagatggct aaccgcgata
     3781 actccgaccc gtccggtctg gggaatacgc tgggatgggc ctgggcgtgg ccgctcaacc
     3841 gtcgcgtgct gtacaaccgt gcttcggcgg atatcaacgg taaaccgtgg gatccgaaac
     3901 ggatgctgat ccagtggaac ggcagcaagt ggacgggtaa cgatattcct gacttcggca
     3961 atgccgcacc gggtacgcca accgggccgt ttatcatgca gccggaaggg atgggacgcc
     4021 tgtttgccat caacaaaatg gcggaaggtc cgttcccgga acactacgag ccgattgaaa
     4081 cgccgctggg cactaacccg ctgcatccga acgtggtgtc taacccggtt gttcgtctgt
     4141 atgaacaaga cgcgctgcgg atgggtaaaa aagagcagtt cccgtatgtg ggtacgacct
     4201 atcgtctgac cgagcacttc cacacctgga ccaagcacgc attgctcaac gcaattgctc
     4261 agccggaaca gtttgtggaa atcagcgaaa cgctggcggc ggcgaaaggc attaataatg
     4321 gcgagcgtgt cactgtctcc agcaagcgtg gctttatccg cgcggtggct gtggtaacgc
     4381 gtcgtctgaa accgctgaat gtaaatggtc agcaggttga aacggtgggt attccaatcc
     4441 actggggctt tgagggtgtc gcgcgtaaag gttatatcgc taacactctg acgccgaatg
     4501 tcggtgatgc aaactcgcaa acgccggaat ataaagcgtt cttagtcaac atcgagaagg
     4561 cgtaaggggg cgaacagatg gctatggaaa cgcaggacat tatcaaaagg tccgcaacta
     4621 actccatcac gccgccttct caggtgcgtg attacaaagc agaagtcgca aaacttatcg
     4681 acgtttccac ctgtatcggc tgtaaagcct gtcaggtggc gtgttcggag tggaacgaca
     4741 tccgtgatga agtggggcac tgcgtcgggg tttacgataa ccccgccgat ctgagcgcca
     4801 agtcctggac ggtgatgcgc tttagcgaaa ccgaacagaa cggcaagctg gagtggctga
     4861 tccgtaaaga cggctgtatg cactgtgaag ctcccggctg cctgaaggcg tgcccgtctg
     4921 ctggtgcaat cattcagtac gctaacggga ttgtcgattt ccagtcggaa aactgcatcg
     4981 gctgtggtta ctgcattgcc gggtgtccgt ttaatattcc gcgcctcaac aaagaggata
     5041 accgggtata taaatgcacg ctctgcgtcg atcgcgtcag cgtcggccag gaaccggctt
     5101 gtgtgaaaac ctgtccgacc ggggctatcc acttcggcac caagaaggag atgctggagc
     5161 tggcggaaca gcgcgtggcg aaactgaaag cgcgtggtta cgaacatgct ggcgtctaca
     5221 acccggaagg ggtcggtggt acgcacgtta tgtacgtgct gcatcacgcc gatcagccgg
     5281 agctgtatca cggtctgccg aaagatccga agatcgacac ctcggtaagc ctgtggaaag
     5341 gcgcgttgaa accgctggca gcggctggct ttattgccac ttttgccggg ttgattttcc
     5401 actacatcgg tattggcccg aataaggaag tggacgatga cgaggaggat catcatgagt
     5461 aagtcgaaaa tgattgtgcg caccaaattt attgatcgcg tctgtcactg gaccgtggtg
     5521 atttgcttct tcctggtggc gctgtccggg atttcgttct tcttcccgac gctgcaatgg
     5581 ctgacgcaaa ccttcggtac gccgcagatg ggacgcattt tgcacccgtt cttcggcatt
     5641 gcgattttcg tcgcgctgat gtttatgttt gtgcgttttg tgcatcacaa catcccggat
     5701 aagaaagata ttccgtggct attgaacatt gtcgaagtat taaaaggcaa tgagcacaaa
     5761 gtggcggatg ttggtaagta caacgccggg caaaagatga tgttctggtc gatcatgagc
     5821 atgattttcg tgctgttggt gaccggggtg attatctggc gtccgtactt tgcgcagtac
     5881 ttcccgatgc aggttgttcg ctacagcctg ctgatccacg cggctgccgg tatcatcctg
     5941 atccacgcca tcctgatcca tatgtatatg gcattctggg tgaaaggatc gattaaaggg
     6001 atgatcgaag ggaaggtaag ccgtcgctgg gcgaagaaac accatccgcg ctggtatcgt
     6061 gaaatcgaga aggcagaagc gaaaaaagag agtgaagaag ggatataatc tcttttgaac
     6121 tttaagctga aaatggcgct ttaagaagcg ccattttttt attgcgtaac cagacggcgc
     6181 agtcgcgaca catccaccgt tttttcagcc tctgccagac tccaggcgtt ttgcagattc
     6241 agccacattt gcggcgaact gccgatcacc acggaaagtt taattgccat ttctggcgtc
     6301 aaagctgctt ttccggtcag caatcggctt gccgttgagg gcgcaatttc cattgctctg
     6361 gcaaactcgc gcaggctgac attaagttcg tccagtgatt cctgaataat gtcccccggg
     6421 cggggatgat tggccatttt cattagtgtc tttactctca gtagcgttcc ttctctggga
     6481 acactatcat tattgttcct tcctacgcaa caataatcca tttagccatt tcatgttgga
     6541 gagtgtgcga tgctgcgacc cgaacatggg agtcgcagca aagacctctt agtgacggaa
     6601 atcaatcacc atacggccac ggattttgcc ttcttccatc tcggtaaaga tggtgttgat
     6661 gtccgctaac ggacgcaggg cgactttcgg caccactttg ccttcggcgg caaactggaa
     6721 ggcttcggtt agatcctggc gcgtgccgac cagcgaaccg accacttcaa tgccatccag
     6781 cacaagacgt gggatatcca ggctcataga ctccggcggc agaccgacag ccacaacacg
     6841 accgcctgca cggacagcat caactgccga gttaaacgca gctttagcta ctgctgttac
     6901 caccgcagcg tgagcgccac cggttttctc ctgcacaatt taggcggcgt cttcggtgcg
     6961 tgagttaatc gctaaatctg cacccatttc ggttgccagt tttaactgct catcattgac
     7021 atcaatggcg atcactttgg cgttaaagac attcttcgcg tattgcaggg cgaggttacc
     7081 cagaccgcca agaccgtaga tagcaatcca ctgaccggga cgaatttttg acagtttaac
     7141 ggctttgtag gtggtgaccc ccgcacaggt aatgctgctg gccgccgccg agtccagacc
     7201 atctggcact tttaccgcgt aatcggcgac cacgatgcac tcttccgcca tcccgccatc
     7261 aacgctgtat ccggcatttt taactgaacg gcagagcgtt tcgttaccac tgttacagta
     7321 ttcgcaatga ccgcatcctt cgtaaaacca cgccacgctg gcacgatcgc ctggttttaa
     7381 tgaggtgaca cctgggccaa cttctgccac cacaccgatg ccttcatggc ccagaattac
     7441 cccagttttg tcaccaaaat cgccattctt aacatgaaga tcggtatgac atacaccaca
     7501 acactccatt ttcagcaggg cttcgccatg tttcagtgag cgcagtgttt tatccgtaac
     7561 gtcaacatga tgatccttcg taacaactgc agccttcata gttcctcctt ttcggatgat
     7621 gttctgcata gcaggtgagg caaatgagat ttattcgcca ctacccagta tggatgagat
     7681 ctgaaaaagg gagagggaaa tagcccggta gccttcacta ccgggcgcag gcttagatgg
     7741 aggtacggcg gtagtcgcgg tattcggctt gccagaaatt atcgtcaatg gcttgttgca
     7801 aagcttcagc agacgttttc accgccacgc cttgctgctg cgccattttg ccaaccgcaa
     7861 acgcaattgc gcgggagact ttctgaatat ctttcagttc cggtagtacc agaccttcgc
     7921 cgttcagcac cagcggcgaa tactgagcaa gcgtttcact tgctgacatc agcatctcat
     7981 cggtgatacg tgacgcgccg gaagcaataa cacccagccc gatgccaggg aaaataaagg
     8041 cgttgttaca ctgggcgata gggtagattt tatctttcca taccactgga ttaaacgggc
     8101 tgccagtggc gaccagcgcg ttaccttcgg tccaggcgat aatgtcctgc ggtgtggctt
     8161 ccacgcgtga cgtcgggtta gacagcggca tcacgatcgg acgcggacag tgtttatgca
     8221 tctcacggat gatctcttcc gtaaacagcc cggtctgtcc tgagacgcca atcagaatat
     8281 ctggttttac attgcgcacc acatccagca gtgacagcac atcgctgtcg gtatcccagt
     8341 cactgaggtt ttcgcgtttc tgcaccagtt tggtctggaa aggcagcagg ttcggcatct
     8401 tgtccgtcag caggccaaag cgatcgacca taaagacttt ctgccgcgcc gcttcctcgc
     8461 ttaatccttc gcgctgggtc tgggcgatga tcatttcggc aatgccgcat cccgctgaac
     8521 ctgcaccaag gaagacgatt tttttctcgc ttaactgacc acctgccgct cggcttgctg
     8581 cgatcagtgt gccgactgtt accgccgcag tgccctgaat gtcatcgtta aaagaacaaa
     8641 tttcattgcg atagcggtta agtaacggca tcgcattttt ttgagcaaag tcttcaaact
     8701 gcaacagcac gtctggccag cgttgtttca cagcctggat aaattcatca acgaattcat
     8761 agtattcgtc gtcaatgata cgcggattac gccagcccat atacagcgga tcgttaagca
     8821 gctgttggtt gttcgttccg acatccagca ccaccggaag ggtatacgcc gggctgatgc
     8881 cgccacaggc ggtatagagc gacagtttac cgatcggaat gcccatcccg ccgatgccct
     8941 ggtcaccaag ccccagaata cgttcaccgt cagtcaccac aatcactttg atattatggt
     9001 tcggtacgtt ttgcagaata tcgtccatat tgtggcggtt ctggtaagag ataaacacgc
     9061 cgcgtgaacg gcggtagatc tcagaaaaac gctcacaggc tgcgccgacg gttggggtat
     9121 aaataacagg catcatctca tcaagatgat tgtttaccag acggtagaag agggtttcgt
     9181 tggtgtcctg gatgttacgc aggtagatgt gtttgtcgat ttcggttttg aatccctgat
     9241 actggatcca tgctcgttcc gcttgttctt cgatggtttc gaccacttcc ggcagtaacc
     9301 ccagcaggtt gaagttacgg cgttcttcca tgctgaaggc actgccttta ttcaacaacg
     9361 gaaattccag cagtacaggg ccagcgtaag ggatataaag cgaacgctgt ttttttgttt
     9421 ttggttccat gtcactcact cttttttgaa tatccatccc tggcacgctt ttatcgtctt
     9481 tgctttaccg ccagggcgtc ggccccaagt ataaagcaga taaaaacaaa aacaccattg
     9541 cgcaggcaat ggtgtttaat cgtcattgag gactgatggt tatgaattac ttttcagcgg
     9601 ggcgttttct gccggttggg ttatttacca cgctggattt gtcaccttcg gtaactattt
     9661 tgcgctggtt agaaatttta tggtccagtc caagaatatg acgtgcctga cggttcgatt
     9721 tcattttaac tcctcaatcc tgttgctagt tttaaggaca acatcgccgt agcgaagaaa
     9781 cacgtgctaa acccctaaat taggttgccg atcaagcata gcaccttaaa gcgtagggtg
     9841 ctggccactg accacataat tgatcgtttg ctggtagata tcactgagga tgtcgttatc
     9901 agaagcttcc acccatttgg tcagctccat gagaatgtca tcttcagtga caacaccgtg
     9961 ctctgcccga aggccttgct caatggcatt aaccagagcg ggttctgctg ttgaattttc
    10021 tgcctgataa taagtaaaca tagtgattct ccgtgtctgt gtatttatgg tgtctgctac
    10081 ggatcgcaga tttataaagc acattcagca tggcaaatat ttgccgcttt gttgttaaga
    10141 ttagtcctgg ttgatgattt ttatatttta acctcatgat attgataggt attattattg
    10201 tggtgagttg attaatatta atgcaatatc tcttgggtta tatattcccg gttatctatt
    10261 gtggaaattt aatttaagtg cagaagtaat atttctgccg gatttttatt cggaatatcc
    10321 tgcttatcct cgtgctgttt ctcacgtagt ctataatttc ctttttaagc ccacatgaga
    10381 gcaacaatga caatccataa gaaaggtcag gcacactggg aaggcgatat caaacgcggg
    10441 aagggaacag tatccaccga gagtggcgtg ctgaaccaac agccgtatgg atttaacacg
    10501 cgttttgaag gcgaaaaagg aactaaccct gaagaactga ttggcgcagc gcatgccgca
    10561 tgtttctcaa tggcgctttc attcatgctg ggggaagcgg gatttacgcc aacatcgatt
    10621 gataccatcg ccgatgtgtc gctggataaa gtggatgccg gttttgcgat tacgaaaatc
    10681 gcactgaaga gtgaagttgc ggtgccgggt attgatgcct ctacctttga cggcatcatc
    10741 cagaaagcaa aagcaggatg cccggtctct caggtactga aagcggaaat tacgctggat
    10801 taccagttga aatcgtaaag cattgccgga tgacgtgtca ggcgcgtgaa tgcctgatgc
    10861 gttgttagca tctctacagc gcccgccagc accggagctc acgtccgtcc tcccttattg
    10921 ctaatgattg ccggacttca catccgctgg ttgcaagggg gcaacgttcg tagaaaaaac
    10981 agccttgcgg caacgtgcgg tttcccggca gatccgtttt acgtaatgcc cattcttcct
    11041 ccagcggttt atcaatggcg gggagggaat ccagcaataa tcgggtgtat ggatgtgccg
    11101 gagccgttag cacctgttgc gcgtcgccca gttctacaat ctgcccgaga tacatcaccg
    11161 ccacccgatc gctcatatga cgtatcaccg agacattgtg tgaaatcagc acataggtca
    11221 gcccgtgatt ttcctgtagc gttaccagta aattgaggat ctgcgcctgc acggagatat
    11281 ccagcgcaga ggttggctca tcaagcacaa tcacgtcagg ctgcgaagag agtgctctgg
    11341 caatggcgat acgttgccgc tgcccgccgg agaacgcatg aggcaggcgg tcgagatact
    11401 ccggacgaat acccacctgc acagccagct cctctaccaa cgctcgccgc tgttgttcac
    11461 tactacgctt agctatccag agcggttctg tgatgatgcg ccacaccggt aagcgcggat
    11521 tgagcgaaga gagcgggtcc tgaaacacca tctgcataat gcgttgtgag ccagaacgga
    11581 tgtactgccc gtggctcggt tgcagcatac ccattaaaag ctgtgcgagg gtgcttttgc
    11641 cgcagcctga ctcgccgacg atccctaagg tttcaccacg gcggatctgt aaatcaatac
    11701 cattaatcgc atgaacatgt tccgtagttt taccaagcca gtttttacgg gccgggaaat
    11761 tgatatggac gccgcgtaac gttaataacg tgtcagacac ttatgacctc ctgctgcgga
    11821 taccaacagg cgcagcgctg gttgttgtca ccacacgctg tcagcgccgg gacgttttca
    11881 cactgtgcac ctgccgcata gcaacgatcg cgaaaagcgc agccgtcagg caaatgggtg
    11941 aggtttggta ccgtccctgg aatggcgggt aatagctggc gtggtactcc atgttctggt
    12001 gcacattgga gcaaaccaat ggtatacgga tgccggggat gatggataac gtctgccctc
    12061 acgccgcttt caattacgct tccggcatac atcacgtaaa cgctatcgca cagttgcgac
    12121 accacggcca tatcatggct gatgaacaga accgcagttc cactggcgcg ggctttatgt
    12181 ttaagcaaac gcagtacctg caactgcacc gtgacgtcca gcgccgtagt cggttcgtcg
    12241 gcaataatca attgcggctc gcaggagaat gccagcgcaa tcattacccg ctggcgcata
    12301 ccacctgaaa gctcaaacgg atagcgcgac ataacttcca cggcatccgg gatttgcatc
    12361 tcttccagca ggacaatcgc tttagctctg gcttcccgac gacttattgg ttgatgatgg
    12421 cggatcacgt ccatcatctg aagacctatt cgacgtgtcg gattgagggc ggtcatcggt
    12481 tcctgaaaga tcatcgccac tcgtgcgccg cgccactgac gaagctgttt ttcccgagcg
    12541 ttaagaacat cgtctcccag cagtgaaatc tgcccccgat gtacgcaata actgcccgtc
    12601 ggtagcagac gcataatcag cattgcggtg actgatttac ctgagccgga ttctcccacc
    12661 agaccgacaa tttcaccgcg gttaatctgc aaggacacat tgttgagcgc gtgaacatca
    12721 ccgttaaaac cggggaaact caaatgcagc tgttgaatgt ccagaacggg ctgggtcatg
    12781 actgctttcc tcctgctttc gggtcaagca gatcgcgaat accatcacca aagagattaa
    12841 acccaacggc ggtaagcaaa atcgctgccc ttggaaatgc gcaataccac cactgatcga
    12901 gtacatagtt gcgaccattc gctaccatcg cgccccattc agcactcggt tgttgagcac
    12961 ccaggccaat aaatcccaac gtggcggcca ttaaaatcgc gctaccgata tccagcgatg
    13021 cctgcacgat cagcggcggc agggagttac gtaaaatatg ccagctgatc agatgccaac
    13081 gcgacgcacc aaaggttttc gccgcctgaa cataggtata ctggcgtaca actaatgctt
    13141 gcccccgcgc caggcgcaca taaaagggaa ttcgcacaat agcaatcgcc agcatggcgt
    13201 taaataaact cggcccgaga gcggcagcca gtgccattgt cagtaccaac gaaggaatcg
    13261 acagcataat gtccatgatg cgcatgatga tggcgtctgc gcgtccgcca agcacaccgg
    13321 atagacatcc gagtagcgaa ccaatcatac ccgcaatggc aaccacgact aatccggcga
    13381 gaattgattg ctgactgccg accagtacgc ggctgaacag atcgcgtccc acttcatcgg
    13441 tgccaaacca gtgcgccgca gaaggcggca aaaggcgggc ggttaaatca atggcgttgg
    13501 gatcatgcgt cgcaagccac ggtgaaaaaa acatcatcaa tagcattaat acaatgatga
    13561 ctgcgccggt cacggtgagc ggactgcctt tcagcatcca taccagtttt gcaccgttaa
    13621 atcgcgtttg tttttgtggg cgtacggcgg acgtttcctc gcttagcatc attcaccacc
    13681 tccacgtccg atacgcggat caatccacaa atagagcaaa tccaccacca ggttgaccag
    13741 cacataagca aatgaaacca cgacggcaaa gcccatcact gccgggaagt cgagcgcctg
    13801 tattgatgtt actacccatg cacccattcc gggccaggca aaaacggttt cggtgagcac
    13861 tgcgccatac aacaaatcgc ccagcgccag acccaatacg gtaatcgatg ggatcaacgc
    13921 attgggtagc gcataacata aaacgatata ccagccgggc aagccgctgg cccgggcggt
    13981 acgaatgtag tcttcactca attgttccag catcgctgag cggatctggc gagcgacaat
    14041 tcccaggtga acgaacgcca gcgttaatgc cggtaagatg agatgttgca acgcattgaa
    14101 gaagacttca ccgttgcctt caagcagcgc atcgagcaga taaaagccgg taacgtgcgt
    14161 tggtggatcc agccagtcat caagccttcc gccgccggga agaatttgca gatgaccata
    14221 aaacagcaca atgacgccca gtccaagcca aaacgcaggt gtggagattc cggtaatggc
    14281 cattattcgc accagatgat ccagccagcg atttcgccag actgcagaga gtatgcccag
    14341 cgggatgcca atcaggagtg ccagcagcag ggcacaaaaa gccagttcca gcgtcgccgg
    14401 gaaaaatata cgcaactctt ccaacaccgg acgcccggta cgaatggatg ttcccaggtc
    14461 accatgaaac aggtcgctga cgtagcggta aaactgtaca tacagtggct ggtccagtcc
    14521 cagttgctgg cgaatatttt ccacgatagc atcgctggcg cggtcacccg ccagtaaccg
    14581 cgccggatcg ccagggatca gatgcgagat aataaacgta atcacgcaga cgcccgccat
    14641 cacgagcacc agcccccagc agcgttggcg taaaatactc cagaaggtca cgttatttac
    14701 tcatggtatt gatattgaag acctgttcca gcatgggatt gaacacaaag cctttcacct
    14761 ctttgttcat cgccagttgg tagtttttct ggaacaggta cacataagca gcgtcatcaa
    14821 tgacgatttt ctgtgcctgc tggtagtccc gggtacgctg cgtctggtcg gtggtcgcaa
    14881 gcgcattgcg cagtaactta tcgacctcac tgttttcata gaacgagcgg ttacccggca
    14941 gacctttttt gtctgactca aaccagtaat tcataaacat atacgggtcg gcaaaatccg
    15001 gactccagtt gccaatcgca atgtcgtaat cacctttacc cactctgtcg cgcatggtgg
    15061 cgttcgccag cttttccagc ttcacattga tgcccagctt gttgagactg gattgtgtcg
    15121 ccagagcaat aggctcccag ttcggatcct tatcggagta gagaaacgtc aggctggtgg
    15181 gtttgctcgt cactttatcc cattcggctt tggctttcgt ttcgtcatgg ttgtattgca
    15241 ttgccgtcgc atcgtagccc cacatgcctt ccggaatcgg gccgcgcatc tgttttccgt
    15301 taccactcag aatgccgtta accatgccct gataatcggt agaccaggaa atggcccgac
    15361 gcagatccgc ctgattaaga ggcgctttgc tgttattcag atacaggtag gtgacgcgca
    15421 gtgacggata ctctgccaca ttgactttgt tttcctgctt cagggcgttg agttgatcca
    15481 ccggcagcgc atcggcaatg tcaatatcac cacgggagag ctgcaggcga cgggaggcac
    15541 tttcaccaat aatttttacc gatactcgct taaagttcgg tttattgcca gggtaatgcg
    15601 gatttggcac cagaactaat tgctgacctt tttgccagct tttcagcata aatggtccgg
    15661 aaccggcggt attttgcgcg aggaagccgc gggcatcatc cgctgcatgt tcctttaaca
    15721 ccgccggatt gataatggat gcaccgtcat tcgccagcgt gtagaggaac ggtgcgaatg
    15781 gctggctaag ggtaaacttc accgtatgtt cgtcgggagc atcaatcttt aaatctttgg
    15841 gaaatgcttc tgctggcccc tggccgattt ttagtagccg ctcaaaagag agttttactg
    15901 cttcggcagt gaccggtgtg ccatcggcaa atttagcatt gtccttcagg gtgaacgtcc
    15961 actctttttg atcgtcagac gctttccagc tacttgccag atcgccttca acgtcggttg
    16021 agcctttatc accgtccgtt ttgtactgaa ccagccgctg ataagacggg taggtcaccg
    16081 tccagtcgtt attatctatt gttaccgccg gggcgagggt ttgtggatcg gcggccttac
    16141 caatcaccag catatctttc ggtacggcgg cgtgcgcaac cgggaaagtt gtggcaagga
    16201 cgagcgcgag caatgtagga cgaaacggta tcgatctctt catgacagtt tcctgattaa
    16261 ctgacgtgct gtgtgccagg ggatataaaa caggtgaatt gatcagcgag cagtgggtaa
    16321 ctcgctgcct ggggtaattc gaagtgccac cattcgctgg agataccgac aaaaccgcca
    16381 ccagtcataa tcgcattcag caacagccga ttgcgctgag cggcgggcgg gacggaaggg
    16441 tgataggcat gggagcgctc gtgcatttca tcgaaccctg cgcccatatc gaggatgttc
    16501 ccgtgctcat cacgaagcgt caggtcgatc gccgtgccac ggctgtgatt agaaccgacc
    16561 gtcacatcaa caacatattg cgggtctggg caggcttgcc acaacatcgc ctgtgcttgt
    16621 tgtgggcgat acgcatcgta aatcaccagt tgtaagcctg acagctgggc gatactgata
    16681 cttttcgcca gcgcggtaat cgcatccttg tgtagcagac aacgcgcttg ctgataaata
    16741 gctttacctg tgatgttatc agcgcaggcg tatttcaatt cgatctccag atcagggaag
    16801 attacggcta aatcaaccag ttcggtggta tccgacataa acggttcctg tataagacaa
    16861 aaattgctgc gctttccgct tatgcagatc tcatgccatg ccgggataag cgccagaatg
    16921 ctggcttaaa gttatatttt ttaacttttg atcaacattt gtgcagcgta gtgcagtttt
    16981 ggtgcaagag gggaagttaa ggaaggaatc tcccggaatc gtagatgaaa tcacagtatt
    17041 taagtgacgg tgtcacgtta aatgaaaacc cgcgagtgcg ggcgagagga atttgtcaga
    17101 ttttcagcgg taacacgctg ctcatccagc ctggaatttc ttcggcgggt agggggcggg
    17161 aaaagaaata tccctgaata acgcgacagt ggatcttgcg tagcatctca aattgctctt
    17221 tggtttcgac gccttccgcc acgacggtta aattgaggct ttgcccaatg ctggtaatgg
    17281 cttcaagtaa ggcaaggatg cgtttttcgg tcagacaacg atcgacaaaa cttttgtcaa
    17341 ttttgatttc cgttaccgga agactgacta agcgggataa tccggaaaag cccgtaccaa
    17401 aatcatctac cgataagccc acgcccatat cacgcaggat ctgaatgcgc ttaaagattt
    17461 cggtatcgtg ttccatcatc atgctttccg tgatttctac cgtcagctgg tggccgtcaa
    17521 taccccaggc gtgcattgca tcagacacct gattaggcag ttgattactg cgaaagtgca
    17581 gtgccgacaa gttaacggaa agcgccggga tatgaatatt ctggctacgc cattctgcta
    17641 actgacggca agcttccgcg atgacccagc gcccaatatt ttcgatttca ccaatctctt
    17701 ctgcgagagg aataaaccgt gaagggggca catgaccatg ctggggatcg tgccagcgcg
    17761 caagggcttc gatgccgtac agttcacccg tttctgcgaa gatttgcggc tggtaaacaa
    17821 gtttcagttg gttattgcta atcgcttctt tcagcgctgc gcctaaaacc aaacgctctt
    17881 ttaccatttc gttcatcgcc gggctgaaga actgccagcc gttaccgcca ttcttgcgaa
    17941 tataatctat tgcattgtga gcagtggaga gtaggtaatc gtggttttta cccacgtcgt
    18001 agctgatgcc aatactcaag gtaagcggga atggtttatc gtcaatcatt atcggcttgc
    18061 tgaccacatt ccgtagctca tcggcgatct gggtaatgtt actgacgtcg ttttccaggc
    18121 tcacgaggac aaactgcgta ccttcgatac gacagagata ctgatccggt ttgagttttc
    18181 cacgaaagcg attgaccact tccagcaatg cctgatcggc ccacgcatag ccaaggctat
    18241 caatcacatc ctgaatatgg tcaacaccga tgagatacac caccggagag acggctttgt
    18301 cgaccaggtc atcgaggtaa ttgtgcaggt tattgcgatt tggcagatcg gtcatcggat
    18361 caaattggat gagttgttca atatgctggc ggcttttttc ctgttccagc gccagcgcgg
    18421 ccatatgctg actgatatct gccacgcgtt cgataaaggc gctggtttct gctcctgacg
    18481 aggttttaat ttgcaggatc cccgcaggcg cgccatcacg ctgacgaatg gtcgctgacc
    18541 agctttgcgc attttgaact tctgcaccgt gcgaagatga cgcccagtgt atcggcatcc
    18601 cgttgcgcag tgcgaacagc gaaacatgcg attcgttgag aacagattcg atgttacgac
    18661 aaatgatttc ccccatttca tgaaatggcg ggctgctgca catggcggcg agaatattgc
    18721 cttcaagctg gcggatttgc cgttcttcgg tgatatccga gaaagtcatt accaggttct
    18781 gcagatgcgc gagcacgtca taaaccgggc tgatagaggc tttaatccag attttttcac
    18841 cggtgcgcgt caacagcaga aattcgtcct gatcgcgggc ggttttccat agcaactgtt
    18901 gtaaacgaat gcggttatcg gcagggaatt caggaatgtt caggagtgta tcgggctgca
    18961 taccgctggc ttcgctaatg cagtaaccaa acatttcggt aaatgcgcga ttgcactgca
    19021 caatatggcg ttccggatcg aggacaatca ccggtcggtc gagatggtca acggcaataa
    19081 tcaattgtcg ggtctgttct ttttgcgcca tttctacgct ggcatcccgt accagcgcca
    19141 ggtaataaac tttcccctcg gcgctcactt tcgatagcgc aaaacgggtc cagattttac
    19201 tgccgtcttt tttctccagc tgcagctccc gactcatccc ctcgacgcgc gctttaccac
    19261 cttcacggtt atgacgaatg tattcaggat gcgcaggacg caaatcccgc ggaatcagca
    19321 tatcaatgtt attgccaatg acttcttcac gtttgtaccc ccagagcttc tctgcggcgg
    19381 ggttgaaaaa catcacttca tcattttcgt taattaacac cgcgcccatc atattttgtt
    19441 caagggcggg gaaaaaaatg ccatcggcgg cattttccgc atcggttagc ttcatgatta
    19501 cctctgcatc ctggcgcatc taaagactgg ctttccagag ttcaacacgg tttctacctc
    19561 gtcttttggc gatatacaga gcttcatcgg ctatttgaat gaggcgctca tagtcaggat
    19621 gaccattaaa catggcggca ccgatggaaa gtgagagggc aatatcctcg ccgtttgcgg
    19681 ctttcagttt ggttttctcc acccgactgc gaatacgttc tgcggtacgt aacgtttcgt
    19741 tttcagaagc ttcagtcaaa acaatgataa attcatcgcc cccgtagcga aaaacataat
    19801 cactactgcg gacgttgtca taaaaggcct gagagacttt acgcagaatt tcatcgccag
    19861 tgttatggcc ccacgtatcg ttgatctctt tgaatttatc aacgtcaata atcagcactg
    19921 acagcggtgt accggtccgg ttggcatggg caatttcgcg tttgaagata gtcggcagga
    19981 agcggcggtt aagtaatttc gtcagtacat ccataccgac ttcgtggcgc gatacttctt
    20041 caaacaattc acgcagcaag gtaataattt gcgatacagt atttcttatc tgtaataaaa
    20101 atttcacccg cagacttctg ttattcaaat tcctggtgtt acgcatggtt tgattgaata
    20161 taccgtcgaa atcctgaatc agacgggaga tatgcctact tcggcaatgc cactaaaata
    20221 atgtcgacct ttatggttaa accacaggcc aaaatcagcc tggcttaaag gcaaactact
    20281 gcctaaatca gaatccagca ggattttata gataatatct atttcccatg aaagtattga
    20341 ggctatttgc cgttcttttt cttcttcggc gttttctagt aacgagaaga tacgatagtt
    20401 ttcatcttcc tttgaggcac tactgtcact aaaggtaaac gcgcgggtca tcacttccat
    20461 agcgatatca atactgttaa tcgagaaatg gtagacctga agtttttctg cggcagaata
    20521 atccgaagag aagattaccg gatagaggat ttttttcagc acccgaaacc ccatctcgac
    20581 aatttctacc ggaattccta tgcgggcatg cacttccgcg acggtatgct ggatttgtat
    20641 tagcctttcg acatcgtcaa cctgggcaga aagcacgtta ataatccagc gttccatcgc
    20701 acttttcaac tgccgctcaa cttgttcatt actcaagaat tcttcggcat gcgggtcgat
    20761 gcggacaatt cgataaaact cgatactcag ataatgagca tgcgcaacgg caatttccgc
    20821 ggctttagca cgaatgagtg gatctgcccg ttcgacaagt ccggtccact catctttcat
    20881 tcttttaaaa tacatctcca taattcacac ccttataagg ctgggaaatc agacggaatc
    20941 aaaacgcaac gtgcgagatc gactaactgc accatattct cctgaaatat gaagatatac
    21001 tgaaaagaaa taagcgactt aggacagttt caatctacgc tactgttctt cagaagagta
    21061 tagcccatcg taattatttt tcggtgacag cgaatatcgt atggtttttc atattcatac
    21121 atttttatta gggatttata gctgtttaac taagtgtggt taattgcgtg gttaatttga
    21181 cttatgtaag catgattact agtgggatat ttcacaaggt gtaacaagcc ggtgggtaaa
    21241 gcaccggctt gttacaaagt aagcatggga gtttaactgc cccaacgact ttgcagatag
    21301 ctgaccgctt gttgagtctg cggtttattc agatagtcct cacggaacaa gatggtgccg
    21361 ctaatttctg gtaccgcatc gttaagatcg agctgctttt tcagttccgg tacgccgccg
    21421 ttaatcatcc agtctggctc tatctttgaa ggttcaccca ctttatagaa ggcgataccg
    21481 atatacaggc gggtcctggt cggtttaacg acatccgccc accattttgc caacacgtca
    21541 taacgcgcgg cactccgtga gaacggccag taaatttggg gagcaatgta atccagcaat
    21601 ccttgttcca cccatcgacg ggtgtcagcg taggattcgt cataggctgc cgcgcctcgg
    21661 gtatcggaac cgagcggatc gtgtgatcgg ttacgccaca cgcctgccgg gctaacgcca
    21721 aattcgactc ccggcttaat gcttttaatg gtgtgcgata cttttgtaat taactgctga
    21781 gtattgttgc gccgccagtc tgcttttgac gcaaatgcgc ctccgtattt acggtacgtt
    21841 tcgttatcat ttagccgtga acccggtgac tccgtataga aatagtcgtc aaactgcacg
    21901 ccatctaccg gatagcggga aaccacttcg gcgactattg atgtgatcca gtcctgaacc
    21961 tcagggatgc ccgggtcgag gacaaagcga tcgccagacg ttctgatcca gtcacggtgt
    22021 tgcacataga cgctcgccgg ttgttgagac agagtgctat tcagttccct gatagtaccg
    22081 ggcttcgtat taaccgatac gcgatagggg ttaaaccagg cgtgtacttt catcccacgc
    22141 ttgtgggctt cgtcgagcat aaattgcagc ggatcgtaac ccggattttc accaatctta
    22201 ccggtcataa gatcggacca cggcaaaatt tttgatggcc acagggcggt accgtccggc
    22261 ttgacctgga aaaagaccgt gtttataccg agacgttgca gatgatccag tttgtcgatc
    22321 atcgcctgtt gttgtacacg ggcccggctg gtggggttac taatgttaac cgaggaaacc
    22381 ggtggccagt cgaggcgaga caccgtggcc agccagatgc cacgcatcgg ttgtgacgat
    22441 tgttgcgtcg tggctggtgg ctttgaacct gctggtggtg tcaccatgga ctctggaggc
    22501 gtgcttttac aactacacag taaaagtgcc agtgcaacca gtatcgctgg tcttctaatc
    22561 gttaatttct tgtttcggga gcagatatcc atatgtgctg gtttccggta aacagatgtg
    22621 cgctcattct cgtacttatc ccggttaagt caatacgaca gcaagcacga aaaagggagc
    22681 gatgaattat cgctcccttg tcttataacc attcagacat ggttagtgtt tcttgtcatt
    22741 catcacaata tagtgtggtg aacgtgcacg cgggtgcagg aagaagtgac cttttggtgc
    22801 gttctgactg ttgattggct ccagagtgac gccggtattc gctttgcctt tacgatcatg
    22861 aacagcatag agaataaagg gcagggcaag taccaccagg aaactaacga ccagtaattc
    22921 aacatacata tcggtagagt caccctggat gttatccggc ggcaggaagg aaacaataaa
    22981 cgccataatt gaagtcagca gaccgacaat tgccacgacc agttttaccc ctttaccacc
    23041 agggatatta aatgtgcgtt ttaagtcagg atgtttaaga actaacacaa tgtagccaat
    23101 aaacagcatg aaataagcac acagataaat caccaccgtc agcgccagtg cgatcaggaa
    23161 ggacatgttg ttaccgccac cggtattggt gaggatgatc aacgcgatag aagtaatcac
    23221 cagctgcgaa atgaccagcg ttaccggtac gccatttttg ttcattttag cgaatgccgc
    23281 tggcagcagg tttttctgcg ctgtgacata catcccgcga gaaggaccaa caatccagga
    23341 ggcgatttcc gccagaacac ccagcaacag cagtgcggag atcacgcgaa ccgtccactc
    23401 aatttccggt gccacatggg acatcagaac ggtaaaggtt tgcattaccc ctgcggagag
    23461 gttgatttca ttacccggaa tgaccatcgc aatagataaa ccgccaacag agcttaagca
    23521 gattgccgcc accatcagca gtaacatagc cagtggatag tcgcgcccgg ggttgctcat
    23581 ttcattgacg tgagttgcgg aagcttctac gcccatataa ctcaaaatga aggcaacaaa
    23641 tacaaccagt gtgcccactt tagagaagtc agggaagaag gtcttcgaat ccatttcgat
    23701 agcaacgggg gcaccggagt gcagataaat agccgctaat gcgatcaaaa taaatgcagg
    23761 taacaggata ccggcgaaga agccaacttt agcaattcgc gccgtgtatt tcgtgccacc
    23821 aaactgcgtt aatgccagcg cccaaagaat gatgagtgct gcaatagttt tggtaatggg
    23881 gtcttcattc agcgctggcc atttcaggat gtaggagagt gcccctaaca cgaaatagag
    23941 catcggaata aaaccaatgg cgatttgcag atagccaaat gagatcgctg caaatcccca
    24001 tctcggcccc agagtatttg atacccaggc gaagacacca ccttcttccc agccgtcgac
    24061 ggtggccatt tccgcagcac aaagtcccac gggaataaac cataaaatcc cgcctaatag
    24121 caggaagaag actaatgaaa agcccgatgt tgcgaaggta gggtattcat aaacagccat
    24181 taccatcgat gccgttatgg caaagaatcc gagtaatgtg agctgcttag ctttacctgt
    24241 ctgtactgat gtagccatat tatcccccta aaacggtatt cctgtcggaa ccgcacctgt
    24301 tttgttctga agcgtattca gaacaatatt ttccgttgct aatgccagtg aacagacttt
    24361 ggaaattgtc ccgaaacggg ttcgtttcgg gacaccgtta ccgttaaacg ttatcaggta
    24421 tgtttaaagc tgttctgctg ggcaataccc tgcagtttcg ggtgatcgct gagatatttc
    24481 agggaggctt tgtagtcttc cagcaacagt tcagcaaagt ccatttcgaa gccgcgacga
    24541 cacataat
//
LOCUS       DAJTMC010000051        24061 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.051, whole genome shotgun sequence.
ACCESSION   DAJTMC010000051 DAJTMC010000000
VERSION     DAJTMC010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24061)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24061)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24061
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.051"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            324..551
                     /gene="feoA"
                     /locus_tag="OO130_001793"
     CDS             324..551
                     /gene="feoA"
                     /locus_tag="OO130_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HCR5365238.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            568..2889
                     /gene="feoB"
                     /locus_tag="OO130_001794"
     CDS             568..2889
                     /gene="feoB"
                     /locus_tag="OO130_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HCR5365239.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAIDETWQSL
                     KDTFSLSVLMNPIEASKGNGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            2889..3125
                     /gene="feoC"
                     /locus_tag="OO130_001795"
     CDS             2889..3125
                     /gene="feoC"
                     /locus_tag="OO130_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HCR5365240.1"
                     /translation="MASLIQVRDLLALRGRMEAAQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            3328..4206
                     /gene="rpnA"
                     /locus_tag="OO130_001796"
     CDS             3328..4206
                     /gene="rpnA"
                     /locus_tag="OO130_001796"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HCR5365241.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPDTARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMGCLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLTDI
                     MRFTGVSEEELAAASQ"
     gene            complement(4235..5005)
                     /gene="bioH"
                     /locus_tag="OO130_001797"
     CDS             complement(4235..5005)
                     /gene="bioH"
                     /locus_tag="OO130_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HCR5365242.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSQGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDDWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVPMPFLRLYGYLDGLVPRKVVPML
                     DKLWPRSKSYIFAKAAHAPFISHPVEFCHLLVALKQRV"
     gene            5043..5726
                     /gene="gntX"
                     /locus_tag="OO130_001798"
     CDS             5043..5726
                     /gene="gntX"
                     /locus_tag="OO130_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HCR5365243.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRAARTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCRWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            5785..6360
                     /gene="nfuA"
                     /locus_tag="OO130_001799"
     CDS             5785..6360
                     /gene="nfuA"
                     /locus_tag="OO130_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HCR5365244.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            6721..8037
                     /gene="gntT"
                     /locus_tag="OO130_001800"
     CDS             6721..8037
                     /gene="gntT"
                     /locus_tag="OO130_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCR5365245.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(8082..10166)
                     /gene="malQ"
                     /locus_tag="OO130_001801"
     CDS             complement(8082..10166)
                     /gene="malQ"
                     /locus_tag="OO130_001801"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HCR5365246.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMTAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLETMFADDGVNKLLKD
                     LDRRRRAAAKKQ"
     gene            complement(10176..12569)
                     /gene="malP"
                     /locus_tag="OO130_001802"
     CDS             complement(10176..12569)
                     /gene="malP"
                     /locus_tag="OO130_001802"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HCR5365247.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQEVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATIGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKLLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLQDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEISEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            13181..15886
                     /gene="malT"
                     /locus_tag="OO130_001803"
     CDS             13181..15886
                     /gene="malT"
                     /locus_tag="OO130_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HCR5365248.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIATVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSRICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKAFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            complement(15929..16945)
                     /gene="rtcA"
                     /locus_tag="OO130_001804"
     CDS             complement(15929..16945)
                     /gene="rtcA"
                     /locus_tag="OO130_001804"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HCR5365249.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITSIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGAVRGGEYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFNTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGSFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(16949..18175)
                     /gene="rtcB"
                     /locus_tag="OO130_001805"
     CDS             complement(16949..18175)
                     /gene="rtcB"
                     /locus_tag="OO130_001805"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HCR5365250.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKDSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAEDLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPVNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIKICLDESDQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQETLETLPSRD
                     LAYFMEGTEYFDDYLKAVAWAQLFASLNRDVMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMTAQSD
                     LVEVIYTLRQVVCVKG"
     gene            18364..19962
                     /gene="rtcR"
                     /locus_tag="OO130_001806"
     CDS             18364..19962
                     /gene="rtcR"
                     /locus_tag="OO130_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HCR5365251.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWHPTLCLCQQESLVIDRL
                     ELLHDARSCSLFETLKRDIASVSPETEVVSIEIELHNPWDFEEVYACLHDFARGYKFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQTSPPRKKEQPRGAGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFFDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHATLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(19944..20702)
                     /locus_tag="OO130_001807"
     CDS             complement(19944..20702)
                     /locus_tag="OO130_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HCR5365252.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(20719..21549)
                     /gene="glpG"
                     /locus_tag="OO130_001808"
     CDS             complement(20719..21549)
                     /gene="glpG"
                     /locus_tag="OO130_001808"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HCR5365253.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVVMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIIAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(21594..21920)
                     /gene="glpE"
                     /locus_tag="OO130_001809"
     CDS             complement(21594..21920)
                     /gene="glpE"
                     /locus_tag="OO130_001809"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HCR5365254.1"
                     /translation="MDLFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            22110..23615
                     /gene="glpD"
                     /locus_tag="OO130_001810"
     CDS             22110..23615
                     /gene="glpD"
                     /locus_tag="OO130_001810"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HCR5365255.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGAISDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(23667..>24061)
                     /locus_tag="OO130_001811"
     CDS             complement(23667..>24061)
                     /locus_tag="OO130_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5365256.1"
                     /translation="VMAGIGWQFSRRTPPELKNRLHCWLVFSPVYFWLILSNFILYIS
                     PEKSALLGNIRNFFLTFVWPPLNFSPFWPQPWTDFVGPISAQLGFALGYYCHWCRKNR
                     NQRKKWGDWVTCLSLAILAQGPLFNYLQ"
BASE COUNT         5655 a         6554 c         6083 g         5769 t
ORIGIN      
        1 accaaactca taatatattc cggcaatatt tatcacttca ttaacaactg aaaccttaat
       61 taaacattag ccagtccggg taattcacta ttcgaattat attttcgctg cgatataacc
      121 ttgagccaca tcaacattga gtcagattat tattcaaacc aacattcgca cacattttaa
      181 gtattgctga tagaaaccat tctcattatc attgtgttat tgattattta atctctcctt
      241 tgttggcaaa tcatctggtc tcatgtcgct gtcaaacgcc ccatgaggta gttatccagt
      301 taatgagaaa caagtaggca cctatgcaat acactccaga tactgcgtgg aaaatcactg
      361 gcttttcccg tgaaatcagc ccggcatatc gccaaaaact gctttctctt ggcatgttac
      421 ctggctcctc ttttaatgtg gtgcgcgtcg ctccactcgg cgaccccatt catatcgaaa
      481 cccgtcgtgt gagcctggta ttacgcaaaa aagatctggc cttattagaa gtggaagcgg
      541 tttcctgtta atacggtgat aacaacaatg aaaaaattaa ccataggctt aattggtaat
      601 ccaaattctg gcaagacaac cttatttaac cagctcactg gcgcacgtca gcgtgtaggt
      661 aactgggctg gcgttaccgt cgaacgtaaa gaagggcaat tctccaccac cgatcatcag
      721 gtcacgctgg tggacctacc cggcacctat tctctgacca ccatctcatc gcagacctcg
      781 ctcgatgagc aaatcgcctg tcactacatt ttgagtggcg acgccgacct gctgattaac
      841 gtggtggatg cgtctaacct tgagcgtaac ctgtacctga cgctacaact gctggaactc
      901 ggcattccct gcattgtggc actgaacatg ctcgacattg ccgagaagca aaatattcgt
      961 attgaaattg atgctctgtc ggcgcgtctg ggctgtccgg tgatcccgct ggtttcaacc
     1021 cgtggtcgcg gtattgaagc gctcaagctg gcgattgatc gctataaagc taacgagaat
     1081 gtggaactgg tgcattacgc acagccgctg ctcaacgaag cagattcact ggcaaaagtg
     1141 atgccttccg acatcccgct gaaacaacgt cgctggctgg gcctgcaaat gctggaaggc
     1201 gatatctaca gccgcgccta cgccggtgaa gcgtcgcagc atctggatgc cgccctcgcc
     1261 cgtctgcgta atgagatgga cgatccggcg ctgcacattg ccgatgcgcg ttaccagtgc
     1321 attgctgcca tctgtgatgt ggtaagcaac accctgacgg cagaacccag ccgtttcacc
     1381 actgcggtag ataaaatcgt gctcaaccgt ttcctcggtc tgccgatttt cctctttgtg
     1441 atgtacctga tgttcctgct ggctatcaac atcggcgggg cgttacagcc gctgtttgac
     1501 gtcggctccg tggcgctatt tgtgcatggt attcaatgga ttggctacac gctccacttc
     1561 ccggactggc tgactatctt cctcgcccag ggcctgggtg gcggcattaa caccgtgctg
     1621 ccactggtgc cgcagattgg catgatgtac ctgttcctct ccttccttga ggactccggg
     1681 tatatggcgc gtgcggcgtt tgtgatggac cgtctgatgc aggcgctggg cttgccgggg
     1741 aaatcctttg tgccgctgat cgtcggtttc ggttgtaacg taccgtcggt aatgggtgca
     1801 cgtacgcttg atgcaccgcg tgaacgtctg atgaccatca tgatggcacc gtttatgtcc
     1861 tgcggcgcgc gtctggctat cttcgcagta ttcgcggctg ccttcttcgg gcagaacggt
     1921 gcgctggcgg tcttctcgct gtatatgctg ggtattgtga tggcggtgct gactggcctg
     1981 atgctcaagt acaccatcat gcgcggtgaa gcgacgccgt ttgtcatgga gttgccggtc
     2041 tatcatgtac cacacgttaa aagcctgatt atccagacct ggcagcgtct gaaaggcttc
     2101 gttctgcgtg ctggtaaagt gatcatcatc gtcagcattt tcctgagcgc tttcaacagc
     2161 ttctcgctga gcgggaaaat cgtcgataac atcaacgact cggcgctggc gtccgtcagc
     2221 cgggtgatca ccccggtctt caagccaatt ggcgtgcatg aagataactg gcaggcaacg
     2281 gttggcctgt ttacaggtgc catggcgaaa gaagtggtag tgggtacgct caacaccctc
     2341 tacaccgcag aaaatattca ggacgaagag ttcaatccgg cagagtttaa cctcggtgaa
     2401 gagctgttca gtgcgataga tgaaacctgg cagagcctga aagacacctt cagccttagc
     2461 gtactgatga accccattga agccagcaaa ggcaacggcg aaatgggtac cggggcgatg
     2521 ggcgtgatgg atcagaaatt cggtagcgca gcagcagctt acagctacct gattttcgtc
     2581 ctgctgtatg taccatgtat ctcggtgatg ggagccatcg cccgtgaatc aagccgtggc
     2641 tggatgggct tctccatcct gtgggggctg aatatcgctt actcgctggc aacattgttc
     2701 tatcaggtcg ccagctacag tcagcaccca acttacagcc tggtgtgcat tctggcggtt
     2761 atcctgttta acatcgtggt tatcggtctg ctgcgccgcg cgcgtagccg ggtggatatc
     2821 gaactgctgg caacccgcaa gtcggtaagc agttgctgcg cagccagcac caccggtgat
     2881 tgccattaat ggcttcactt attcaggtgc gcgatttgct ggcgttacgg ggccgtatgg
     2941 aagcggccca gataagccag acattgaaca ctccacagcc aatgattaac gccatgctgc
     3001 aacaactgga aagtatgggc aaagccgtgc ggattcagga agaacctgac ggctgcctct
     3061 ctggcagttg taaaagctgc ccggaaggaa aagcctgtct gcgcgagtgg tgggcgcttc
     3121 gttaacctta ctccatcgcc tgttttggaa agcccggtat gcgtctgcat ccgggctttt
     3181 ttgcgtgcgg ctttccataa aaatgcaact cttgcagcac ggcgtaagtt cctttgaaag
     3241 catctcgcag ggatgaaaac tcgctaatac acaggtgtgg agtggcgcgt agagtcgcgg
     3301 cattcaaaca acaggtgaag gaacgccatg agcaaaaagc agagttccac cccacacgat
     3361 gcgctgttca aactcttttt acgccaaccg gacacggctc gtgattttct tgcgtttcat
     3421 ttaccggcac ccattcacgc gctttgtgat atgaaaaccc tcaagctgga gtcgagcagc
     3481 tttattgatg acgatctgcg tgaaagctat tccgatgtgc tgtggtcggt gaaaacggaa
     3541 caaggaccag gatacatcta ttgtctgatt gaacatcaaa gcacctcaaa caaactgatc
     3601 gcatttcgca tgatgcgtta cgctattgcc gcaatgcaaa atcaccttga tgctggatac
     3661 aaaacgttgc cgatggtggt gccattgttg ttttaccacg gtattgaaag cccctatccc
     3721 tattcgctgt gttggctgga ttgtttcgcc gatcccaaac tggcaaggca gctttatgcc
     3781 tccgcatttc cgctgattga tgtcaccgtc atgcctgatg atgaaatcat gcagcaccga
     3841 cgtatggcgc tgctggagtt aattcaaaaa catattcgtc aacgcgacct gatggggcta
     3901 gtagagcaaa tgggctgctt attaagtagt ggatacgcta atgacagaca aatcaaaggg
     3961 ctgtttaatt acatactgca aaccggcgac gcggtacgtt ttaacgattt tatcgacggc
     4021 gttgccgaac gttcaccgaa acacaaggag agtttaatga caattgcgga aagattgcgg
     4081 caggaggggg aacaatccaa agccctgcat atagccaaaa taatgcttga atccggagtc
     4141 cctcttacag acatcatgcg ctttaccggg gtgtcagaag aagagttggc tgcggcgagc
     4201 cagtaaattt ctgtctcgcc atttcaaaag ttgcctacac cctctgcttc aacgccacca
     4261 gcaggtgaca aaactcgacc ggatgcgaaa taaacggcgc atgggccgct ttggcgaaga
     4321 tatatgattt gctgcgaggc cagagtttat ccagcatcgg caccactttg cgcggcacca
     4381 gaccatcgag atagccatac aatcgcaaaa acggcatggg cacgttttgc agcggctgac
     4441 ggagatcaac cgttttcagg atttccagcc cgccattaag cacgtcaacc tccggcatcg
     4501 gtaacgccag aacggttttc ttcaacgccc gcgcatcctg gcgcgccgtt tcagtcccca
     4561 tggtttgtaa cgccaggaac cgctccactg tacgctgaaa atcatcactg agttgctgct
     4621 gaaatcccgc cagcacgtcc ggttttatcc ccggccaatc gtcacgagca ctaaaacaag
     4681 gtgacgacgc cacggtgacc agcgcctgaa cacgctcggg atgggttaac gcaatctggc
     4741 ttgccaccag cccgcccaga ctccagccta accaaatggc tttatcaggt gcctgttgca
     4801 gcacggcttc ggccatatca gcaagtgaca gcgcaccaaa tccctggctg cgcccgaagc
     4861 cgggcagatc aacaaggtgc agcgtaaaat gcgagctaag ttcctcgtca atgcaacgcc
     4921 acacttcggc attcagtccc catccgtgca gcagcacaag atgaacattc ccctgacctt
     4981 tggtctgcca ccagatgtta ttcatccgct attgttctct tttgacttac aaggatgaac
     5041 atatgctaac agtaccggga ttatgctggc tatgccgaat gccgctggcg ttaggtcatt
     5101 gggggatttg ttcggtctgc tcacgcgccg cccgtacaga taaaacgtta tgcccacaat
     5161 gtggattacc cgccacacac tcacatcttc cctgcggtcg ctgcctgcaa aaaccaccgc
     5221 cctggcaaag actggtcacg gttgccgact atgcgccgcc gttaagtccg cttatccacc
     5281 agcttaaatt ttcccggcgc agtgaaatcg ccagcgccct gtcacgtctg ttactattgg
     5341 aagtcttaca cgctcgtcgc accaccgggt tgcaattgcc ggatcgcatc atcagcgttc
     5401 cgttatggca gcggcgtcac tggcgtcggg gatttaatca gagcgatttg ctgtgtcagc
     5461 cgttatcacg ctggttgcac tgccgatggg atagcgaagc cgtcacacga acacgggcca
     5521 ctgcgaccca gcattttctc agtgcccggc tacgcaagcg caacctgaaa aatgcctttc
     5581 gtcttgaatt gcccgtgcaa ggtcgccata tggtgattgt ggatgatgtc gttaccaccg
     5641 gaagtaccgt cgcagagatt gcgcagttgc ttttacgcaa tggtgcggcg actgtccagg
     5701 tctggtgcct ttgtcgaacc ttgtagagcc tcgatgatag gcgtattata accaactaaa
     5761 atagtcaact attaggccat tactatgatc cgtatttccg atgctgcaca agcgcacttt
     5821 gccaaactgc tggcaaacca ggaagaaggg acacaaatcc gcgtatttgt gattaaccct
     5881 ggcacgccta acgctgaatg tggtgtttct tattgtccgc cggatgccgt ggaagccacc
     5941 gacactgccc tgaaatttga cctgctgacc gcgtatgttg atgagttaag cgctccttac
     6001 ctggaagatg cagagatcga ttttgttacc gaccagttgg gttctcagct aacactgaaa
     6061 gcgcctaacg ccaaaatgcg taaagtggca gacgatgcac cactgatgga gcgcgtggag
     6121 tatatgctgc agtcgcagat caacccacag cttgctggtc acggtggccg cgtttcactg
     6181 atggaaatca ccgaagacgg ttacgccatt ctgcaatttg gcggcggctg taacggttgt
     6241 tccatggtcg atgtgacgct gaaagaaggg atcgagaagc agctgctaaa cgaattcccg
     6301 gagctgaaag gtgtgcgcga tctcaccgaa caccagcgcg gcgaacactc ctactactaa
     6361 gttatcccct cttttgtaga ttgcccgatg cgacgctaaa gcgtcatttc gggcctacgt
     6421 tgaacatgtg ccagacgtgg gcgcagcatt gctttggcaa atccctccct tcgtttctcc
     6481 gcgataatat gaccaacctc tcataattta aatttactcc gctctggtga ttctcaaacg
     6541 ccagatgtta ccggtatcat tcacatgggt accaaacata ctcctgacat ctgactacaa
     6601 taattagttt tagtgggtat cagtcgtggt gccgcaatat ctctgttccc gattgggata
     6661 attagagttt gtcgtcagaa aattgacgtt acccgtaaca aatgaaaggc caggtaaatc
     6721 atgccattag tcattgttgc tatcggtgta atcttgttgt tgctcctgat gatccgtttc
     6781 aaaatgaacg gcttcatcgc tctcgtcctc gtggcgcttg ctgttggatt aatgcaaggg
     6841 atgccgctgg ataaagttat tggctccatc aaagccggtg tcggcgggac gctcggtagc
     6901 cttgccctga tcatgggttt tggcgcgatg ctgggcaaaa tgctggcaga ctgcggtggc
     6961 gcacaacgta tcgccaccac cctgattgcc aaatttggta aaaaacacat ccagtgggcg
     7021 gtggtactga ccggttttac cgttggtttt gccctgttct atgaagtggg ctttgtgctg
     7081 atgctgccgc tggtgtttac catcgcggct tctgcgaata ttccactgct gtatgttggt
     7141 gtaccaatgg cagctgcact gtctgtgacc cacggcttcc tgccaccgca tccgggtccg
     7201 actgcgattg ccaccatttt caatgccgac atgggtaaaa ccctgctgta cggtactatt
     7261 ctggcaatcc cgaccgtgat tctcgccggt ccggtttacg ctcgcgtgtt gaaaggtatc
     7321 gataagccga tccctgaagg tctgtacagc gcgaaaacct tcagcgaaga agagatgccg
     7381 agctttggcg tcagcgtctg gacctctctg gtgccggtag tgctgatggc gatgcgtgcg
     7441 attgccgaaa tgatcctgcc gaaaggtcac gctttcctgc cggtagcgga gttcctcggt
     7501 gacccggtaa tggctacgct gattgccgtg ctgattgcga tgttcacctt tggtctgaac
     7561 cgtggtcgtt caatggatca gattaacgac acgctggttt cttccatcaa aatcattgcg
     7621 atgatgctgt tgatcatcgg tggtggcggt gcgttcaagc aggtgctggt agacagcggc
     7681 gtggacaaat acattgcttc catgatgcac gaaaccaaca tttctccgct gctgatggcc
     7741 tggtcgattg ctgccgtact gcgtatcgcg ctgggttccg caaccgttgc ggcaatcact
     7801 gcgggtggta tcgcggcacc gctgattgca acgacgggtg ttagcccgga gctgatggtt
     7861 attgcggtgg gttccggtag tgtgattttc tctcatgtga acgatccggg cttctggctg
     7921 ttcaaagagt actttaacct gactatcggc gagaccatca aatcctggtc gatgctggaa
     7981 accattatct cggtgtgcgg tctggtaggc tgtctgctgc tgaatatggt gatttgataa
     8041 cactgactgc cggatgcatc gcatccggca tcaaccgcac tctactgctt cttcgctgca
     8101 gctctgcgcc gtctgtccaa atccttcagc aacttattca cgccatcatc ggcaaacatc
     8161 gtctcaagcg ttgcggaaag cttgcgtcgc cagtttttat actggtaact ggtgccagga
     8221 atattcaccg gttcggccat atccagccag tcttccggct gtagtcctaa cagagcactg
     8281 ttactgtcgg caatgtagcg ctgcaaacca cgattaagcg tcggcgtcat cgacatcaac
     8341 gatgccttat gcccggcacg ttttggcaga caaccatatt tatgcagtgc atccagcagc
     8401 ccttgcttcg ccagttcgcg atcctgatac agaccgcgca gtaccacttc atccggatac
     8461 agccccaggg ttttgcccaa cgttagatcc ccgctctccc agtaaccgcg cagcgtaggc
     8521 aggtcatgtg tcgccgcaac cgccatcgac tgctccggat acgctttcgg tgcacggaac
     8581 gtcttctcgt ggtcgttttc gaaatagagc actttgtagg agtacacacc gctgctgcgc
     8641 agcttaccga caatctctac cggtacggta ccgagatctt caccaatcac catacagcga
     8701 tgacgtttac tttcgagtgc cagaatcgag agcagatcat ccaccggata gtgaacatac
     8761 gcgccctgat ctgccgtctc gccatacggt atccaccaca aacgcagcat cgacatcaca
     8821 tggtcaattc gtaatgcgcc gcagttttgc atattggcac gcaacagctc gataaacggt
     8881 tcataggcac gcgcggtgat gatatgcggg tccattggcg gtaatcccca gttctgcccc
     8941 aacgggccga ggatatccgg cggcgcgcca accgacgctt tcaggcaata tagttcacgg
     9001 tcacaccagg tttccgcccc accttccgct acgccaaccg ccagatcacg atacaagcca
     9061 atcggcattt catagccctg gcttatctcc cagcaggcgg caaactggct gtaagccagc
     9121 cactgcaacc agagataaaa atcgacgtca tcacgatgtt cttcgcagaa ctgacgcact
     9181 tctggtgaat ccacgttctg atacatctct ggccatgcag gccagcccca gcgcatttcg
     9241 tcctctttca cttgctgggc atgtagcgca tcaaaggctg cctgccagaa caggctgtcg
     9301 ccctgctctg caacaaactg gcgaaacgcg gtcatctgct catcatcacg ttgcgcgaaa
     9361 cctttccacg ccattcgtaa tgctgtcatt tttagggcgg taaccgtgga gtaatcgacc
     9421 cagtcggcat cgcgcgcctg ttgcagcgtc tgttgcgtgg tcggcaactg ccaccaggcc
     9481 tgagcctctt cgctaagatg gaaatcttca acggcgttaa cgtcgatata aatcacattc
     9541 agccaacggc gagaagacgg actgtatggg ctggcactct ccggatttgc cggatagagc
     9601 gcatgaatcg ggttcaggcc gatgaacgac ccgccacgtt ttgccacatc caccagcatc
     9661 gctttgagat cgccaaaatc cccaataccc cagttttttt ccgatcgcag cgtataaagc
     9721 tgaacgcagg caccccacag cttttgttta ttcagcaacg cctgcggttc gtaacagcgt
     9781 ttcggggcga caatcacccg gcaatgcgcg cgctggtcgt cctgggtgag tgtcagcgtg
     9841 tgataacctt ccggcagctt cgtcggtaga ttgaacgctt tgccccccgt tacatggcct
     9901 ttgtactgtg ttccttcttc ggtggtcagc agccagctat attcgccgct gccctccacc
     9961 accatcggca tttttttgcc gctggtataa accatgacat tcgggactgg cgttaccgcc
    10021 actttcgtgg cggtacgttg atgcatcgcg tcaagcaaac gccgtttggt ttcggcgcta
    10081 atcgactgcg gtttaccgtg ggcattgatg taattggggc taatccccgc cgccagcgcg
    10141 gcattatcca gacgtttgct ttccatcgag cttccttagc gttttgcctg ccagatacga
    10201 gcctgataat cgcgaataga gcgatccgag ctaaacatac cgcagcgggc ggtattgagg
    10261 atcgccgcgc gagtccaggc ctcctggtcg cggtacagca catccacctg cttttgtgcc
    10321 tcaacatagg ctgcgaaatc cgccatcacc agatacggat cgccgccttg tttgccgata
    10381 ctgtgcagca tctggtcgaa ggcatgctta tcgccgtcgc tgtatttacc gctttccagc
    10441 tctttcaata ctgcgtccag caccttatct ttcttccgcc atttcaccgg gtcgtagcct
    10501 ttggccagaa ttgccttcac ttgttccacg gtatgaccaa aaataaagat attttcttca
    10561 ccgactttct cggagatttc aacgttcgca ccatccagcg taccgacagt aagcgcacca
    10621 ttgagcgcca gtttcatatt gccggtaccg gaagcttctt tacctgcagt cgaaatttgt
    10681 tcggagatat ccgccgccgg gatcagtttt tccgccgccg aaacgcaata atcctgcagg
    10741 aacaccacct tcaacttatc gccaaccagt ggatcgttgt tgatcacgtc agccactttg
    10801 ttgatcgcaa agataatatt cttcgccagg tagtagcccg gtgccgcttt cgcgccgaag
    10861 aggaagacgc gcggtacgcg atcagcctgc gggttttcac gaatttcttt gtacaacgcc
    10921 agaatatgca gcagattcag gtgctggcgt ttgtactcat gcagacgttt aatctgaata
    10981 tcgaaaatcg cctgtggatt gatctcaata ccggtacgaa ctttcacaaa ctccgccaga
    11041 cggactttat tcgcctgctt gatctcgcga tattgctgac ggaatttcgc atcatcagcg
    11101 aatttttcca gattgatcag ctgatcgaga tcgttagccc actctttttg cagtaattta
    11161 tccaacagag ccgccagtgc agggttgcac tgtttgatcc agcgacgtgg ggtaataccg
    11221 ttggtgacgt tatggaattt gttcggccat agctggtgat attccgggaa cagatctttc
    11281 accaccagat ccgagtgcag cgccgcaaca ccgttcaccg cgaaaccgcc aaccacacac
    11341 aggttcgcca tatgcacttg tttatcgtgc accaccgcca gtttggccca cactttttca
    11401 tcgcccggcc aggttttctc caccagcgtt ttaaagcgag tattaatttc gttaataatc
    11461 tgcatatggc gcggcagtaa gcctttcacc agtttcacat cccagcgttc cagcgcttct
    11521 ggcatcaggg tatggttggt gtaagcgaaa gttttgctgg taatggccca ggcgtcatcc
    11581 cagctcatct ggtgctcatc gatcagcacg cgcagcagtt ctggaatcgc gatagttggg
    11641 tgggtatcgt tcagctgaat aacttcataa tccgccagtt cgtgcagttt acgccccgcc
    11701 agatgatggc gacgcaaaat atccgctacc gaacaggcac actggaagta ctgctgcatc
    11761 aagcgcagct ttttaccggc agtatggttg tcgtttggat agagaacttt ggtcagtttt
    11821 tccgcattga tgccctgctg ttcggcacgc aggaaatcac cgtcgttaaa tttagtcaga
    11881 tcaaacggat gcgcgtgcgt cgcctgccac agacgcagcg gctgcgccac gccattacga
    11941 tagccgacaa cggggagatc ccacgcttga ccggtaatgg taaactccgg ctcccagcgt
    12001 ccgtctttcg tcactttacc gccaatccct acctgcacat ccagtgcttc gttgtggcgg
    12061 aaccacgggt agttactacg atgccagtca tccggcgctt caacctgttt gccatcgaca
    12121 aaagactggc ggaacaaacc atattgatag ttcagaccgt aacccgtcgc agactgaccg
    12181 atagttgcca ttgagtcgag gaagcacgcc gccagacgtc ccagaccacc gttacccagc
    12241 gccgggtcga tctcttcttc cagcaggtcc gtcagattga tgtcataagc cttcaacgaa
    12301 tcctgtactt cctgatacca gccgagattc aacaggttgt tgcccgtcag gcgaccaatc
    12361 aaaaactcca ttgagatgta gttaacatgt cgctgattcg ccaccggctt ggcgaatggc
    12421 tgagcacgca gcatttcggc cagtgcttca ctcactgcta gccaccactg gcgaggagtc
    12481 atttcagccg cagaatttaa gccataacgc tgccactgac gtgaaagcgc ttcctgaaat
    12541 tgcttatcgt taaaaatagg ttgtgacata ggagttccac ttttcttaga ttttcaacac
    12601 aacgttatcg ctagtttgcc aggctcgatg ttgaccttcc tcatcctgcg ggggattagg
    12661 cagggaggag ttgcggggat gagcaaggaa atgtgatctc aaccacttaa agctagtgca
    12721 aaccacagga ttagcagcaa atcaatgcaa tacagcgcag aaaatctgta tctaagtgca
    12781 aaaaatggcc gttgcgtatt ttcaaaaagc ggaaggtaac tctataaatt aagtaaagga
    12841 gtgaaacagt ttcacaagta aaatatccag tgtgctccat ctcattctta atagatttat
    12901 taagatcatc tttttagatg gcactttcat caggaatgaa gaagaaaccc ttgcttaaat
    12961 gaatctgatg aacataaggg aaaccagtat tcacgctgga tcagcgtcgt tttaggtgag
    13021 ttgttaataa agatttggaa ttgtgacaca gtgcaaattc agacacataa aaaaacgtca
    13081 tcgcttgcat tagaaaggtt tctggccgac cttataacca ttaattacga agcgcaaaaa
    13141 aaataatatt tcctcatttt ccacagtgaa gtgattaact atgctgattc cgtcaaaact
    13201 aagtcgtccg gttcgactcg accataccgt ggttcgtgag cgcctgctgg ctaaactttc
    13261 cggcgcgaac aacttccggc tggcgctgat cacgagtcct gcgggctacg gaaagaccac
    13321 cctcatttcc cagtgggcgg caggcaaaaa cgatatcggc tggtactcgc tggatgaagg
    13381 tgataaccag caagagcgtt tcgccagcta tctcattgcc accgtgcagc aggcaaccaa
    13441 cggtcactgt gcgatatgtg agacgatggc gcaaaaacgg caatatgcca gcctgacgtc
    13501 actcttcgcc cagcttttca ttgagctggc ggaatggcat agcccacttt atctggtcat
    13561 cgatgactat catctgatca ctaatccagt gatccacgag tcaatgcgct tctttattcg
    13621 ccatcaacca gaaaatctca ccctggtggt gttgtcacgc aaccttccgc aactgggcat
    13681 tgccaatctg cgtgttcgtg accaactgct ggaaattggc agtcagcaac tggcatttac
    13741 ccatcaggaa gcgaagcagt tttttgattg ccgtctgtca tcgccgattg aagccgcaga
    13801 aagcagtcgg atttgcgatg acgtttccgg ttgggcgacg gcactacagc taatcgccct
    13861 ctccgcccgg cagaataccc actcagccca taagtcggca cgccgcctgg cgggaatcaa
    13921 tgccagccat ctttcggatt atctggtcga tgaggttttg gataacgtcg atctcgcaac
    13981 gcgccatttt ctgttgaaaa gcgccatttt gcgctcaatg aacgatgcgc tcatcacccg
    14041 tgtgaccggc gaagaaaacg ggcaaatgcg tctcgaagag attgagcgtc aggggctgtt
    14101 tttacagcgg atggatgata ccggcgagtg gttctgctat cacccgctgt ttggcaactt
    14161 cctgcgccag cgctgccagt gggaactggc ggcggagctg ccggaaatcc accgtgccgc
    14221 cgcagaaagc tggatggccc agggatttcc cagcgaagcg attcatcatg cgctggcggc
    14281 aggcgatgcg ctgatgctgc gcgatattct gcttaatcac gcctggagtt tgttcaacca
    14341 tagcgaactg tcgctgctgg aagagtcgct taaggccctg ccgtgggaca gtttgctgga
    14401 aaatccgcag ttagtgttat tgcaggcgtg gctaatgcaa agccaacatc gctatggcga
    14461 agttaacacc ctgctagccc gtgctgaaca tgaaatcaag gacatcagag aaggcaccat
    14521 gcacgcagaa tttaacgctc tgcgcgccca ggtggcgatt aacgatggta atccggatga
    14581 agcggaacgg ctggcaaaac tggcactgga agagctgcca ccgggctggt tctatagccg
    14641 cattgtggca acctcggtgc tgggtgaagt gctgcactgc aaaggcgaat tgacccgctc
    14701 actggcgcta atgcagcaaa ccgaacagat ggcacgccag cacgacgtct ggcactacgc
    14761 cttatggagt ttaatccagc aaagtgaaat tctgtttgcc caagggttcc tgcaaaccgc
    14821 gtgggaaaca caggaaaaag cgttccagct gatcaacgag cagcatctgg aacagctgcc
    14881 gatgcatgag tttctggtgc gcatccgagc acaactgtta tgggcctggg cacggctgga
    14941 tgaagccgaa gcgtcggcgc gtagcgggat tgaagtcttg tcgtcttatc agccacagca
    15001 acagcttcag tgcctggcaa tgttgattca atgctcgctg gcccgtggtg atttagataa
    15061 cgcccgtagc cagctgaacc gtctggaaaa cctgctgggg aatggcaaat atcacagcga
    15121 ctggatctct aacgccaaca aagtccgggt gatttactgg caaatgaccg gcgataaagc
    15181 cgccgctgcc aactggttgc gtcatacggc taaaccggag tttgcgaaca accacttcct
    15241 gcaaggtcaa tggcgcaaca ttgcccgcgc acaaatcttg ctgggcgagt ttgaacctgc
    15301 ggaaattgtt ctcgaagaac tcaatgaaaa tgcccgcagt ctgcggttga tgagcgatct
    15361 caaccgtaac ctgttgctgc ttaatcaact gtactggcag gccggacgta aaagtgacgc
    15421 ccagcgcgtg ttgctggacg cattaaaact ggcgaatcgc accggattta tcagtcattt
    15481 tgtcatcgaa ggcgaagcga tggcgcaaca actgcgccag ctgattcaac tcaatacgct
    15541 gccggaactg gaacagcatc gcgcgcagcg tattctgcga gaaatcaatc aacatcatcg
    15601 gcataaattc gcccatttcg atgagaattt cgttgaacgt ctgctaaatc atccggaagt
    15661 gccagaactg atccgcacca gcccgttgac ccaacgtgaa tggcaggtcc tggggctgat
    15721 ctactccggt tacagcaatg agcaaattgc gggcgagctg gaagtggcgg caaccaccat
    15781 caagacgcat atccgcaatc tgtaccaaaa actcggcgtc gcccaccgtc aggccgcggt
    15841 acaacacgcg cagaaactgc tgaagatgat gggatatggg gtgtgagttt agccggataa
    15901 tgcattggat ccggtttaca tctttacatc attcaatact cacccgcgtt acgccatctg
    15961 tttctatcaa accaaaccgc accggcaaga aacgctccac cacggcgata ttggtcagca
    16021 gatggcatga aggatgggcg accgtaaatt ctcccgcgcc cgccagcgcc atcggtagca
    16081 ccagctggtc ggcgagatat tcccccactg ccgccgggct tgccaggtag cgtttcacct
    16141 ctttcactaa ctgcgcggct accacctcgg cactgacgcg cttttcaccg acgacgaaaa
    16201 agcgttcagt gatattttca ctttcgactt ccagcgagac ggtattaccc ggcccctgat
    16261 cgcgcggcag gttatgaata ttctgttcat gcagggaaaa actccccgcc agtgtagcga
    16321 tttcacgctc agcaacatgg cgcggcacac cagctaatag aacttctcca cgcatctgca
    16381 caatgttccc gcgctcgcca agttgcaagg tgttaaacga tgccacgggc gagacttccg
    16441 ttgccaccac gccgcctccg gcaggataaa aaccgtgacg taacagcgtg gtttgctgat
    16501 gaattcctat tttcgccagc agcggctcca gcacccggcg gataaaatcc gcaggcggcg
    16561 ccgacgggtt atcggtgcct ccgctcactt caacacgcga aggtccatcg gcaaaccaca
    16621 gcgcgggcag caccgtttgc agcaccagcg tacagcttcc ggcactaccg atagcaaagc
    16681 ggtactcgcc gccgcgcacg gcgccaggcc ggaagaccag acgctgcgac cccagctccg
    16741 cgccttccac cgttgcccta caaatttccg cagccgcttt taccgcggtc agatgctggc
    16801 gcaacagccc cggtttcgcc cgcccggcac gaatgctggt gatggtaaat ggctggccgg
    16861 ttatcatcga caggctcagc gccgagcgca gaatctgccc gccaccttcg ccctgtgcgc
    16921 catccagcgc aatcatcctt ttcatcattt atccttttac gcacaccacc tgacgcaggg
    16981 tatagataac ttccaccaga tcgctttgtg ccgtcatcac cgcatcaata tctttatacg
    17041 ccatcgggat ttcgtcgatc acttcggcat ctttacggca ttccacatgc gcggtggcac
    17101 gaatttgatc ttccacgctg aacagttttt tcgctttagt tcggctcatt acccgcccgg
    17161 caccgtggct gcacgaacag aacgactctt catttcccag cccacggacg ataaagcttt
    17221 ttgctcccat cgaaccggga ataattccat attgaccagc acgcgcagac accgcgcctt
    17281 tacgcgtcac gtagatctct tcaccaaagt gctgttcttt ttgcacatag ttgtggtgac
    17341 agttgatctc ttccatcgcc agcgtttgtg gctgtctgac cgttttctgc gtaatgctct
    17401 gcaatgccgt taccacgttt tccatcatca catcgcggtt aaggctggca aaaagctgcg
    17461 cccaggccac ggctttcagg taatcatcaa agtattccgt accttccata aagtacgcca
    17521 gatcacgcga cggcaacgtc tcaagcgttt cctgcatctc tttttgtgcc agatcgataa
    17581 agtaagtccc gatggcgtta ccaattccgc gtgaaccgga gtgcagcata atccacacct
    17641 ggtccgactc atcaaggcag attttaataa agtggttacc ggttcccagc gttcccaggt
    17701 gtttatagtt attggtattc aggaaacggg gatatttttg cgttaaccac tgataaccgg
    17761 cttcaagctc agcccatttc gcatcgacgt tgacaggcgg gttttcccat gcgcctttat
    17821 cacgtttaca acgcccggta gtacgcccgt gcggcacggc cgtttcaatc gcctgacgca
    17881 gctctgccag gttttcaggc aggtcttccg ccgttaacgc ggtacgcagc gcgttcattc
    17941 cacagccaat atccacgccc accgccgccg gaataatcgc ccctttggtc gggatcacgc
    18001 taccaatggt ggaatcttta cccaggtgta catcaggcat taccgcaata tgtttgaaaa
    18061 taaacggcat cttcgccgta ttaataagtt gctgacgcgc atcggcttct accggcacgc
    18121 ctttggtcca catttttacc ggggcatttt cagtggtcag taattcgtaa ttcattttgt
    18181 ttttctcttt tcgttgtttg ctgtcctgat aattgcaacc gtcgtgccag aaaattaaat
    18241 aagtaaatta attttttaat tcattgtttt taaaaagatt atatctttat gtccgtaacc
    18301 ggagatttcc cgcaaagccg atttaccgat aatgaaatat cgtcttttat aaggatatct
    18361 aagatgcgta aaacagtagc ttttggcttt gtcggaacgg tgctggatta tgccgggcgc
    18421 ggcagtcagc gctggtcaaa atggcatccg acactctgtt tatgccagca agaatcgttg
    18481 gtcatcgatc gactggaatt attgcatgac gcccgctcgt gctcgctatt tgaaacgctt
    18541 aaacgcgata tcgccagcgt ttcgccagaa acagaagtgg tgagcattga gattgaactg
    18601 cataacccgt gggatttcga agaggtttac gcctgcctgc atgatttcgc ccgtggttac
    18661 aagtttcagc cagaaaaaga agactattta attcacatca ccaccggcac ccacgtcgcg
    18721 cagatttgct ggtttctgct ggcggaagcg cgttatctac ccgcccggtt gatacaaact
    18781 tcaccaccgc gcaaaaaaga acaaccgcgc ggtgccggtg aagtgacgat tatcgatctc
    18841 gatctaagcc gttataacgc catcgccagc cgctttgccg aggaacgcca gcaaacgctt
    18901 gattttctta agtccggcat tgccacgcgt aacccccact tcaaccgcat gattgagcag
    18961 attgaaaaag tggcgattaa atcccgcgca ccgatcctgc ttaacggccc aaccggtgcg
    19021 ggaaagtcat ttctggcgcg acgtatattt gaattaaaac aggcgcggca tcagtttagc
    19081 ggcgcgtttg tggaagtgaa ctgcgccacc ctgcgcggcg ataccgccat gtcgacgctg
    19141 tttggtcatg tgaaaggcgc gtttaccggg gcgcgggaat ctcgtgaagg tttattacgc
    19201 agcgccaacg gcggaatgtt gttttttgat gagattggcg aactgggcgc agacgaacag
    19261 gcaatgctgc taaaagccat tgaagagaaa accttttacc cgtttggcag cgatcgccag
    19321 gtgagcagtg attttcagct tatcgccgga acggtgcgcg atttgcgcca gctggttgcc
    19381 gaaggcaaat ttcgcgaaga tctgtacgcg cggatcaatc tctggacctt caccctgccg
    19441 ggtctacgcc agcgccagga agatattgaa ccgaaccttg attatgaagt ggagcgccac
    19501 gccacactca ctggcgacag cgtgcgtttt aacaccgaag cgcggcgcgc ctggttggct
    19561 tttgcgacct ctccccaggc aacatggcgc ggtaactttc gcgaactttc tgccagcgtc
    19621 acgcggatgg ccacctttgc cactagcgga cgcatcactc tggacgtggt tgaagatgag
    19681 ataaaccgtc tgcgctataa ctggcaggag agccgcccct ccgcgcttac ggcgttgctg
    19741 ggcgcagagg cagaaaacat cgatctcttc gaccgtatgc aactggaaca cgttatcgct
    19801 atctgccgcc aggcaaagtc gctttctgca gccggacgac agctttttga tgtttcgcgc
    19861 cagggaaaag ccagcgtcaa tgacgcggat cggctacgca aatacctggc gcgttttggt
    19921 ctgacgtggg aagccgtgca ggatcagcac agctccagtt gaatatgatg gtccgtcagc
    19981 acctgcatta cgcttactgg cggcggggcg tcggtgtaga cggcatctac catgctgatg
    20041 ctgcccatat tgaccatcgc gttacggcca aatttcgagt gatcgacaac cagcataacg
    20101 tggcgcgagt tctcaataat ggcgcgcttg gtgcgaactt cgtggtaatc gaactccagc
    20161 agcgagccgt cgctatcgat gccgcttatc cccagaatgc cgaaatcaag gcggaactgg
    20221 gagataaaat cgagcgtcgc ttcgccaatg atcccgccat cgcggctgcg taattcgcca
    20281 ccagcgagaa taatgcggaa atcttctttt accatcaacg tgttagcaac attgagattg
    20341 ttggtgacaa tacgcaaatt gctgtgatta agcagcgcgt gcgccaccgc ttccggcgtg
    20401 gtgccgatat cgataaacag tgtcgagccg ttggggattt gctccgccac tttgcgggca
    20461 atcctctctt tttcttcggt ctgggtggct ttgcgatcgt gccacggcgt gttaaccgaa
    20521 ctggaaggca gcgccgcacc gccatggtga cgcaggatca ggttttgctc cgccagctca
    20581 ttgaggtcgc ggcgaatagt ctgcgggctg acggagaaat gctctaccag ctcttcagta
    20641 ctgacataac cctgctgttt aaccagttcg ataataccgt tgtgacgttg tgtttgtttc
    20701 atttataaat ccctggaatt attttcgttt tcgcgcattg agcgaatcaa caaaagccat
    20761 cgctaaaccc acggctaacc cggcgatgtg tgctccgttc gccatcgaca tcccaaacaa
    20821 atcaaaccat ccggcgataa tccagatcag cgcaaagata attaacccac gttgcaggta
    20881 aatgccactt tgcggatcgc gctcaccacg tagccagacg tagcccatca gcgcatacac
    20941 cacgccagaa agcccgccaa accacggccc gctgaatttt tgctgcacat agccgcttaa
    21001 cagggcacta atgagagtaa taacaattag cttaccgcta ccgaggcgtt tttccaccgc
    21061 accgccgaga taccaccacc agagcaggtt aaagaggata tgcatcagcg agaagtgcat
    21121 taacgcgtgg gtgaagtaac gccagaactc aaatttcagt gttggatcga atggccaggc
    21181 cagccataac atcacttcct gatcgccgag aatttgcatg gcgataaaca ccaccacgca
    21241 ggcgatcatc accacccagg ttaccggacc tgcgcgttca cgcaaggcag caaagaaagg
    21301 ataacggcga taatgcagac cactgccggt atggcccgcc tgccagctgg ccgccagata
    21361 acgcggatct gccgggtttt cgagaaaacg cgccagctcc gcccgcacgc gctcggcctg
    21421 ggactcatcc gccagccaga catcgctttg gttatgttgt tgaatcgtaa ggataacacc
    21481 ctgcgtcgcc atgtaatcaa caaacgcctg cgccacgcgg gggttagcaa aagaggtaat
    21541 catcaacatc gttgctgtcg cttattccac acaaaagggg acagtataaa gcgttacgcg
    21601 ccgtacgcca cctctgcggg aaactgacgc tgccaggctt caaaaccgcc gtcaatgcta
    21661 tagaccacat cgtagccctg ttgcagcaga tactgcgccg cgcctttgct gctattgccg
    21721 tgataacaca tcaccatcac cggagtgtca aagtcgttat cacgcataaa cgcgcccagc
    21781 gtgtcgttgg ttaaatggaa agcctgcacc gcatgtccca ttgcgaaact ctgtggatcg
    21841 cgaatatcga ccagcaccgc ctctttttcc tgcaacttct ggtgcgcgtc ggcaacgtta
    21901 atacattcga acagatccat gcgtctctct ttctttacaa acaagtgggc aaatttaccg
    21961 cacagtttac gtcgaagcgg cagataaacg ccataatgtt atacatatca ctctaaaatg
    22021 ttttttcaat gttacctaaa gcgcgattct ttgctaatat gttcgataac gaacatttat
    22081 gagctttaac gaaagtgaat gagggcagca tggaaaccaa agatctgatt gtgataggtg
    22141 gcggcatcaa tggtgctggt atcgcggcag acgccgctgg acgcggttta tccgtgctga
    22201 tgctggaggc gcaggatctc gcttgcgcga cctcttccgc cagttcaaaa ctcattcacg
    22261 gtggcctgcg ctaccttgag cactatgaat tccgcctggt cagcgaggcg ctggctgaac
    22321 gtgaagtgct gctgaaaatg gctccgcata tcgccttccc aatgcgtttt cgcctgccac
    22381 atcgtccgca tctgcgcccg gcgtggatga ttcggattgg tctgtttatg tacgatcatc
    22441 tgggtaaacg caccagcctg ccgggatcaa ccggtttgcg ttttggcgca aactcagtgt
    22501 taaaaccgga aattaagcgc ggattcgaat attctgactg ttgggtagac gacgcccgtc
    22561 tggtactcgc caacgcccag atggtggtgc gtaaaggcgg cgaagtgctt actcggactc
    22621 gcgccacctc tgctcgccgc gaaaacggcc tgtggattgt ggaagcggaa gatatcgata
    22681 ccggcaaaaa atatagctgg caagcgcgcg gcctggttaa cgccaccggc ccgtgggtga
    22741 aacagttctt cgacgagggg atgcatctgc cttcgcctta tggcattcgc ctgatcaaag
    22801 gcagccatat tgtggtgccg cgtgtgcata cccagaagca ggcctacatt ctgcaaaacg
    22861 aagataaacg tattgtgttc gtgatcccgt ggatggacga attttccatc atcggcacca
    22921 ccgacgtaga gtataaaggc gatccgaaag cggtgaagat tgaagagagt gaaatcaact
    22981 atctgctgaa agtgtataac gcgcacttta aaaaacagct tagccgtgac gatatcgtct
    23041 ggacctactc cggtgtgcgt ccgctgtgtg atgatgagtc cgactcgccg caggctatta
    23101 cccgtgatta cacccttgat atccatgatg aaaacggcaa agcaccgctg ctgtcggtat
    23161 tcggcggtaa gctgaccacc taccgaaaac tggcagaaca tgcgctggaa aaactgacgc
    23221 cgtattatca gggtattggc ccggcgtgga cgaaagagag cgtgctgccg ggtggtgcca
    23281 ttgaaggcga ccgcgacgat tacgcagctc gtctgcgccg tcgctatcca ttcctgactg
    23341 aatcgctggc gcgtcattac gcacgcactt acggcagcaa cagcgagctg ctgctcggca
    23401 atgcgggggc gataagcgac ctcggggagg atttcggtca tgagttctac gaagcggaac
    23461 tgaaatacct ggtggatcac gaatgggtcc gccgcaccga cgatgccctt tggcgtcgca
    23521 caaaacaagg catgtggctg aatgcggatc aacaatctcg tgtgagtcag tggctggtgg
    23581 aatatacgca gcagaagttg tcgctggcgt cgtaaggatg atgcccggtt gaagtgcaac
    23641 cgggctattt ggattcctta gactattcat tgtaaataat tgaataacgg cccctgagct
    23701 aaaatcgcca aacttaagca cgttacccaa tcgccccact tcttcctctg gtttctattt
    23761 tttctgcacc aatggcaata atatcccaat gcaaaaccaa gctgtgcact aatcgggccg
    23821 acaaaatcag tccaaggctg cggccaaaaa ggggaaaaat tcaggggagg ccagacaaac
    23881 gtcagaaaga aatttcggat atttcccagc aacgctgatt tctctggaga aatataaaga
    23941 atgaaattcg agagaattaa ccagaaatag accggcgaaa agaccagcca gcaatgcagt
    24001 ctgtttttta attcaggagg cgttctgcgg gagaattgcc aaccgatgcc agccattaca
    24061 c
//
LOCUS       DAJTMC010000052        23492 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.052, whole genome shotgun sequence.
ACCESSION   DAJTMC010000052 DAJTMC010000000
VERSION     DAJTMC010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23492
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.052"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(297..611)
                     /gene="pspE"
                     /locus_tag="OO130_001812"
     CDS             complement(297..611)
                     /gene="pspE"
                     /locus_tag="OO130_001812"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HCR5365257.1"
                     /translation="MFKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            complement(686..907)
                     /gene="pspD"
                     /locus_tag="OO130_001813"
     CDS             complement(686..907)
                     /gene="pspD"
                     /locus_tag="OO130_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HCR5365258.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(916..1275)
                     /gene="pspC"
                     /locus_tag="OO130_001814"
     CDS             complement(916..1275)
                     /gene="pspC"
                     /locus_tag="OO130_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HCR5365259.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(1275..1499)
                     /gene="pspB"
                     /locus_tag="OO130_001815"
     CDS             complement(1275..1499)
                     /gene="pspB"
                     /locus_tag="OO130_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HCR5365260.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(1553..2221)
                     /gene="pspA"
                     /locus_tag="OO130_001816"
     CDS             complement(1553..2221)
                     /gene="pspA"
                     /locus_tag="OO130_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HCR5365261.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKESGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            2388..3365
                     /gene="pspF"
                     /locus_tag="OO130_001817"
     CDS             2388..3365
                     /gene="pspF"
                     /locus_tag="OO130_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HCR5365262.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPATVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFATQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEDAIAVSETTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(3485..4750)
                     /gene="puuE"
                     /locus_tag="OO130_001818"
     CDS             complement(3485..4750)
                     /gene="puuE"
                     /locus_tag="OO130_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HCR5365263.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLKNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALSD"
     gene            complement(4788..6057)
                     /gene="puuB"
                     /locus_tag="OO130_001819"
                     /pseudo
     CDS             complement(4788..6057)
                     /gene="puuB"
                     /locus_tag="OO130_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
     gene            complement(6059..7546)
                     /gene="puuC"
                     /locus_tag="OO130_001820"
     CDS             complement(6059..7546)
                     /gene="puuC"
                     /locus_tag="OO130_001820"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HCR5365264.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FSDCSDLQKAASSTAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDCAHADSVHSFIREGEGKGQLLLDGRNAGLAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEEQALQLANDSQYGLGAAVWMRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(7821..8378)
                     /gene="puuR"
                     /locus_tag="OO130_001821"
     CDS             complement(7821..8378)
                     /gene="puuR"
                     /locus_tag="OO130_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HCR5365265.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(8405..9168)
                     /gene="puuD"
                     /locus_tag="OO130_001822"
                     /pseudo
     CDS             complement(8405..9168)
                     /gene="puuD"
                     /locus_tag="OO130_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
     gene            9380..10798
                     /gene="puuA"
                     /locus_tag="OO130_001823"
     CDS             9380..10798
                     /gene="puuA"
                     /locus_tag="OO130_001823"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HCR5365266.1"
                     /translation="METNIVEVENFVQQPEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQKMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGYLQPPCAPGTDNRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKLLVRLMAEKHKMHSTFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            10947..11112
                     /locus_tag="OO130_001824"
                     /pseudo
     CDS             10947..11112
                     /locus_tag="OO130_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            11102..12487
                     /gene="puuP"
                     /locus_tag="OO130_001825"
     CDS             11102..12487
                     /gene="puuP"
                     /locus_tag="OO130_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HCR5365267.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSINLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YDGDRTPVSET"
     gene            12621..12866
                     /locus_tag="OO130_001826"
     CDS             12621..12866
                     /locus_tag="OO130_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HCR5365268.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVADAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            13179..14822
                     /gene="sapA"
                     /locus_tag="OO130_001827"
     CDS             13179..14822
                     /gene="sapA"
                     /locus_tag="OO130_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HCR5365269.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLKFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            14819..15784
                     /gene="sapB"
                     /locus_tag="OO130_001828"
     CDS             14819..15784
                     /gene="sapB"
                     /locus_tag="OO130_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapB"
                     /protein_id="HCR5365270.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            15771..16661
                     /gene="sapC"
                     /locus_tag="OO130_001829"
     CDS             15771..16661
                     /gene="sapC"
                     /locus_tag="OO130_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HCR5365271.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMLAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            16661..17653
                     /gene="sapD"
                     /locus_tag="OO130_001830"
     CDS             16661..17653
                     /gene="sapD"
                     /locus_tag="OO130_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HCR5365272.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            17655..18461
                     /gene="sapF"
                     /locus_tag="OO130_001831"
     CDS             17655..18461
                     /gene="sapF"
                     /locus_tag="OO130_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HCR5365273.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            18529..18882
                     /locus_tag="OO130_001832"
     CDS             18529..18882
                     /locus_tag="OO130_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HCR5365274.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            19250..20038
                     /gene="fabI"
                     /locus_tag="OO130_001833"
     CDS             19250..20038
                     /gene="fabI"
                     /locus_tag="OO130_001833"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HCR5365275.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            20183..21310
                     /locus_tag="OO130_001834"
     CDS             20183..21310
                     /locus_tag="OO130_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HCR5365276.1"
                     /translation="MEQRHITGKSHWYHETQSSTAEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTARDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLEIQNYADASLFADESIRWRSSYEV
                     EKLPEEYTKSSTAELCQLAETLSLHPISLSLLERLLNSTRVNTQPDNKLAALLCARIN
                     GSPACFAACMDSSNEYKKISPLLRKGENEINQWADRHSVEHATVQAIQWLTRAPDRFS
                     TAQFSPLLEHEQSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            21378..23312
                     /gene="rnb"
                     /locus_tag="OO130_001835"
     CDS             21378..23312
                     /gene="rnb"
                     /locus_tag="OO130_001835"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HCR5365277.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGAIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTDTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAMRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(23390..23492)
                     /locus_tag="OO130_001836"
                     /pseudo
     CDS             complement(23390..23492)
                     /locus_tag="OO130_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         5311 a         6211 c         6114 g         5856 t
ORIGIN      
        1 gacgcgttac aagtttctgt aaatgagttg cggtaaatgt tccaccgtag atttcgtcca
       61 ggtaatcagt aattttctgt ttcatgttgc ctttcagtgg tagcgatccc atgaaacgag
      121 agactactcc gcagaaaata aactgtcaat tgagcacaag ggttttagtc acgcaggaaa
      181 tcatttttta ccagggaaaa agcgtatttg tgagctgacg caaataatct atggggatgt
      241 aaataaggtg tgacagaaaa aaaacggcgc ataagcgccg ctcatggtga attcttttaa
      301 cctttgacct tcggcattgc gatgtctttc aagccaccgg cgttctcaac gtgggtatat
      361 cccatctcgc taaggatctc ttttgcttgc cctgactggc gtccggcatt gcaatacact
      421 ttcacggtgt cgtttttatc cggaacggcg gtggcaatgc gctctttcac ttccttcagg
      481 ggaatattga tggccccctg aacgtgctct tgctgatact gctctggaac acgaacatcg
      541 atccagtgtt cagcggcgaa aacgggcagt gaaaacacca gtgccagagc aagtaagcct
      601 tttttaaaca tgtaagcgtc cttctatgga tgaaaattgc catccattat acgcctgaga
      661 tttaacaatt tttgcgaaac tcccctcacc ttttataacg ctgtgccagt ttattagcag
      721 cccgacttaa cagcggttcc agcgccacag ccagcaacat tttcagcggt cggcgagcaa
      781 ctgattttat cgcccagccc gctacacccg ccgggccata gcgcagtgcg gtaagaagta
      841 ccagcttgcc tgctaatttg aaaccaggct ttaccttttg cccggcctgt tgccagcgag
      901 tattcataac tttcctcaca gttgacggaa acggctacgt aacgtgaaag tatcggaagt
      961 gacataacgt tccatctcgc gtaaacgcgt ttcacttgcc gccagttcac ggtcgacttc
     1021 atccagcaat tcgctgctgg agggtagctg ctcaccaaag gccatgttgt ccggcattgg
     1081 atcaagcgca aatgacaaaa tgatgtaagc aaccagggta aacagcgcca gaccgaagaa
     1141 aatcgacagc accaccagga tacgcaccag ttttaccggt acatcaaaat agttggcaat
     1201 cccggcgcag acgccgcgga ccatgccctg ctgtggaata cgccataatt ttttattgag
     1261 attaatgccc gccattagcg atccctccag ttcggatgtt ctgcatcaag aatagattcc
     1321 agcgcctgaa tacgttcgcg catccgtttt gcttcatcag ccagttgcgc taatcgctgc
     1381 tgctcacttt gcgacaattc actgcgacca gaacgattgc tgtaatgcag ccataaccag
     1441 atcggtaaaa caaacagcac aaaaatggtt aacggaatag ccagaaatag cgcgctcata
     1501 agtactcctt agacgatgag cggcggcgcg ttcggacgcc gcctagatgt tattattgat
     1561 tgtcttgctt cattttggct tttaattgtg ccagttgttc gctgattgca tcatcggctt
     1621 tcagttcggc aaactgatcg tccagcgatt tttgtttacc gaagctgtgg ctttctgctt
     1681 ccgcttccat ctggtcaata cgacgttcga aagattcgaa acgagccatt gcttcatcca
     1741 gtttgccact gtccagctga cgacgcacat cgcgcgacga gtttgccgcc tgatggcgta
     1801 acatcaatgc ctgctggcga gcgcgtgttt cgctcaattt gttttccagc tcaccgctct
     1861 ctttcttcat gcgtgccagc gtatcgtcca ccagcgtcac ttcatgttcc agactcttaa
     1921 tcagatcggt cagtttctgt ttttcaatta acgccgcacg tgccaaatcc tctctctctt
     1981 tcagcagcgc cagttcggct ttttcctgcc attcaacttc acgcgccgac gcttgttcaa
     2041 tacggcgagt cagctgtttc ttttctgcca acgcacgcgc cgaagtagaa cgtacttcaa
     2101 ccagtgtatc ttccatctcc tggatcatca gacgcaccag tttctgtgga tcttccgctt
     2161 tctctaacag agcgttgatg ttggcattca cgatgtcggc aaagcgagaa aaaataccca
     2221 taatgttgtc ctcttgattt ctgcgttcag gattgtcctg ctgaactgtt aatacaattt
     2281 gcgtgccaat tttttatctt tttgatttat aaagatctga ttgaagaatc atcagcaaca
     2341 tgccaggatg aattagcgaa ttacactaac aagtggcgaa tttcatcatg gcagaataca
     2401 aagataattt acttggtgag gcgaacagct ttctcgaagt gctggaacag gtttcgcatc
     2461 tcgcaccgct ggacaaaccg gtgctcatca tcggcgaacg cggcaccggt aaagagctga
     2521 ttgccagccg cctgcattat ctctcctccc gttggcaagg gccgtttatt tcccttaact
     2581 gcgcggcgtt aaatgaaaat ctgctggatt ccgaactgtt tggtcacgaa gcgggggcgt
     2641 ttaccggtgc gcaaaaacgt catccaggga gatttgaacg tgccgacggc ggtacgctat
     2701 ttcttgatga actcgctacg gcaccgatga tggtgcagga gaaattattg cgcgtgattg
     2761 agtacggtga actggagcgc gttggcggca gccagccatt gcaggtgaat gtgcggttgg
     2821 tatgcgcgac gaatgccgat ctcccggcga cggtcaatga aggcactttt cgcgctgacc
     2881 tgctcgaccg actggctttt gatgttgtac aactgccacc actgcgcgag cgcgaaagcg
     2941 acataatgtt gatggcagaa cactttgcca cccagatgtg tcgggaaatc aagctgcctc
     3001 tgttcccggg gtttacggag cgcgccagag aaacattgct gaattatcgt tggccgggaa
     3061 atattcgtga attgaaaaac gtggtggaac gttcagtgta tcgccacggc accagcgatt
     3121 atccgcttga tgacatcatt attgatccct ttaaacggcg tccgcctgaa gacgctatcg
     3181 ccgtttcaga aaccacctcg cttccaacac tgccgctgga tttacgtgag tttcagatgc
     3241 agcaggaaaa agagttgctg caactcagtt tgcaacaagg gaaatataac cagaagcgcg
     3301 cggctgaatt actggggtta acttatcatc agttccgcgc gttgttgaaa aagcaccaga
     3361 tttagcgaag ttgagcaata caacttgccg gatgcggcgt gaatgcttta ttcggcatac
     3421 ataatcaata agtcgtttgc aggcctgata agcgcagcgc atcagacatt attgcgttgg
     3481 gctattaatc gctcagcgca tcctgcaaaa ttttcattgc cgcatcgaat tgcgcatccg
     3541 gaatggtcag cggatacagg aagcgaatca cgttgccgta tgcgccacag gtcagcagga
     3601 gcagcccctg cgccagcgcg cgttgctgga ttttctgtgc aatcgccgct gacggctcgc
     3661 ccgtttgcgg atcgttaaac tctgccgcaa tcatcgaccc caggccgcgt accgcagcaa
     3721 tggccggaac gctttctttg gcatcaatca acgtgttttt gagacgctgg cccagttgat
     3781 tcgcgcgttc gcagagtgat tctttgtcga taatattgag caccgcgtgc gcggcagcca
     3841 ccgccagcgg gttcccggcg taggtgccac ccaacccgcc cggcgcgggc gcgtccataa
     3901 tattcgcgtt accgaccacg cccgaaagcg gcatcccgcc cgcgaggctt ttcgccatcg
     3961 tcattaagtc cggcttatcg gcgtaatgat ccatggcaaa cagtttaccg gtacgcgcaa
     4021 agccgctttg cacttcatcg gcaatcatca caataccgtg ctcgtcgcac aggcggcgaa
     4081 tggcggcaac cagctctttt ggcgcaacgt tgaaaccgcc ctcgccctgc accggttcga
     4141 aaataatggc cgccacctgc ttcgcttcaa tgtccgattt aaacaagcgt tcgatggcgt
     4201 cgagggagtc ctgtgttgaa atgccgtgta aatctgacgg ataaggtacg tgatacaccg
     4261 aaccagggaa cgggccgaag ccgattttgt acggcgcaac ttttccggtc agcgccatgg
     4321 tcatatatgt acgaccgtgg aagccgccgc taaacgcaat cacgccaggt cgtccggtat
     4381 gggcgcgagc aattttcacc gcgttttcca ccgcttccgc gccggtagtg aagaatgcgg
     4441 ttttagcctg cccgctcact ggggcaaggg cgttaatttt ctccgccagc gtgacgtagc
     4501 tttcgtacgg cacaatctga tacgccgtgt gggtaaactg ttgcaattgc tgctccaccg
     4561 ccgcgaccag atcagggtgg cgatgcccgg tattcagcac cgcaatgcct gcggcgaaat
     4621 cgatgtattc gttgccctct acgtccttca gcgtagcgtt ttctgccgac tgggcgaaga
     4681 agttacacat cacgccaacc ccgcgtgggg tagcagaaag acgacgctga tggaattcat
     4741 tgttgctcat gtttcacacc ttttgactct tcactgttaa tcggaaatta aacgcccaga
     4801 cgatcgcgca ggctgtaata agccgcgccc atcgcggtaa atggcacgcg cagcgtgcgc
     4861 ccgccgggga acgggtaatg cggcagattg gcgaaggcat cgaaacgctc ggcgtcaccg
     4921 cgcagcagtt cagcaatcaa acgcccggcg aggtgggtac aggtgacgcc gtggccgctg
     4981 tagccctgca tgtaatagat gttggtatcg aggcgaccga actgcggcat ccgtgaaagg
     5041 gtcagcagga agttgcccgt ccagcgatag tcgattttga cgcccttcag ctgcgggaag
     5101 gttttcagca gtttcggcac aacaagacgc tcaacgtcat ccgggtcgcg cgcgccgtag
     5161 accacgccgc cgccgtacag caaacggttg tcggcggtaa gacggtagta atcaagcaga
     5221 taattacagt cttccacaca gtagtttttc gggatcagcg aacgggctaa atcttccgac
     5281 agccgttcgg tggtgatcac ctgcgtgccg cacggcatgc tgcgtttcgc cagttccggc
     5341 tctactttat cgcccagata cgcattcccg gcgacaatca catacttcgc cgtcacctga
     5401 cctttggcag tgcgcacaac ggctggcgtg gtgtgctgga tctgcgtcac ggcagaaagt
     5461 tcatacacgc gcccgccgtt gaggcggatg gcgtccgctt cgccaatggc gaggtttagc
     5521 gggtgaatat gcccaccgct gtgatccagc agcgcaccgg tatagcgatc gctggctact
     5581 tcacggcgaa tggcgttggc gtccagcaat tccagctgtt tattgccgta gcgttcccag
     5641 ttctctttct gctcttcaag tgtggcgagc tgtttatcat tcatcgccac aaacaggctg
     5701 ccggggcggt agtcgcagtc aatctgataa cgtttgatac gttcgcggat gatctcacca
     5761 ccttcgaaca tcatgctgcc gagcataggg gctgtgtcca tgccgtagct tttttcgatc
     5821 acgtcgatgt cgcggttgta ggagttcaca agctgcccgc cattgcgccc gcttgcgcca
     5881 aagccgatgc gtgaggcttc gagaaccact acgtcaaagc ccgcttccgc cagatgcagc
     5941 gcggaggaga gcccggtata gccgccgcca accacgcaaa cgtcgcaggt gattgactca
     6001 ttcagggtgt cgaatggcac gtatttatgc gtaataactg ctggtatgtt cggtcatttc
     6061 aggcctccag gcttatccag atggttttca gttcggtgaa tttttcaagg gcatgcaggg
     6121 atttgtcgcg accgttgccg ctctgcttat agccgccaaa cggcacggtc atatcgccgt
     6181 cgttgtagtt attgacgaag acggaaccgg ctttcaggcg gcggctcata cggtgcgcgc
     6241 gggagaggtc gcgcatccat accgccgcgc caaggccgta ctggctgtcg ttggcaagct
     6301 gtagcgcctg ttcttctgat gtgaaacgcg tgaccaccag caccggaccg aaaatctctt
     6361 cgcggcttaa ggacgcattc gggtctacat ccacaaagat ggtcgggccg atggcggcag
     6421 ccagcccggc gttacggcca tccagcagca gttgcccttt gccttcgccc tcacgaataa
     6481 agctatggac cgagtcggcg tgggcgcagt cgattaaagt gcccatggtg gttgcggggt
     6541 caagcggatg gcccggctgc cagttttgcg cctgctgttt taacagggct aagaattcat
     6601 cggcgatgct ctcttccagc aacaggcgtg ttccggcgat gcacacctga ccctggttgt
     6661 agaagatgcc tgctgcggtg gagctcgccg ctttttgcag atccgaacaa tcggagaaaa
     6721 cgatgttggc gcttttgccg cccgcttcca gccagacgcg tttcatgttg ctgtcgcccg
     6781 catctttcag cagctgtttc ccggtacggg ttgaaccggt aaaggcaatg gcgtcgatat
     6841 cgttatgacg cgacagcgcc tgccccgctt catgaccaaa acccgtcacc acgttcaaca
     6901 caccatccgg caagcctgct tctttcgcca gccccgcgag acgaatcgca ctgagcggtg
     6961 atttttcaga cggttttaga atcacgctgt ttcccgccgc cagcgccggg ccgagtttcc
     7021 agcaagtcag caacagcggg aagttccacg gcacgatggc ggcaatcacg ccgaccggtt
     7081 cacgcacgat catcgccagc tcatggctac tggtggtcgc cacttcgcca tacactttgt
     7141 cgatcgcttc ggcgtaccag cgaatggcgc gcgccgcgcc gggaatatca tcacgcagac
     7201 tgtgacgaat cggtttgccg gtgtcgagag tttccagcag tgccagctct tcggcgtggg
     7261 cttccattaa atcggcgagt ttattcagta ccgctttacg tttagccgga gaagagagtg
     7321 accagtcgcc gcgttcaaat acgccgcgtg ctgcgctcac cgcacggtcg atatcgacgc
     7381 tcttgccgcg ggcaattttc gccagcggtg cctgggtgac tggatcaacg gtttcaaagg
     7441 tttcattttc cgccgcagca gtatattcac cgttaataaa taagcggttt tcaatggcga
     7501 gacttaacgc tttatcctgc cagtaagcca gatgatgaaa attcattatg actcctgttt
     7561 cacgtctatc agatatatgc agtttgtgaa cgtcagggcg cagagatccc cgggccgtat
     7621 gcccgtttat ttctggcgac attctttata tacacttcat ccttcaagcc gcctctgcct
     7681 tggctgcgtt cgctcccccg aatcacttac ctgagtaagc tcatcgggat tcgctcactt
     7741 gccgccttga tgcaacttga atgattttgt gtatagattg ccgggcgcat tgcgcttacc
     7801 cggcttacag aacaaaaaga ttaaaacgtg gtgggcgtat gggcgctgat aattcggcaa
     7861 atacctgccg acgtattact gaaactgtgc gggatgccgg tattaatggc ataactttga
     7921 cccgcgacga ggtggtaatc ctgaccatta atcgtcagaa caatttcacc ttccagtact
     7981 gtgcctattt cctcaccctg atgcttaatt ctttccccag tggttgtgcc cggctggtac
     8041 gtttcaaaga tcatcgccag cgtgcgattc gggttaccgt tatgaaccag cttcattgac
     8101 acaccctgac tgcccatctc aattaagtcg tcctgattaa tgacgacctg cggctcatca
     8161 ggtttttccg gctcggaaaa gaattccgag agtgacagac catacacctt cagcagcttt
     8221 tgcagcgtac tgatggcagg gctgacttta tcttgttcta tcgtactgat agcactgtga
     8281 gtcagcccgg agagttcggc ggcacgacgt tgtgaaagcc cctgctgctg gcggatttcc
     8341 gacaagcgtt ttcctggcgc cagtccctca tcactcatat ttgcatttcc ttaaactgta
     8401 gtggtcagag tcgctgtttt tcagcgatat ggtgctgaca agcggtgata aagccctcga
     8461 acaatatacg cgaaagagcg tactcgctac tgttccattc cgggtgccac tgtacgccca
     8521 gcgcaaaagg atgattgatg acgctaaccg cctcaactaa gccatccggc gagcgagctt
     8581 caacacgcaa ccgtgggcta acgaccttcg ccccttgtcc atgtagagag tttacccaaa
     8641 agttgctaca ttcaggtaac aacgcagaca gtaatccccc ctcttcaacc tgaacttcat
     8701 gcgacggtgc atattgctgt tccaccggca gttcggatct tcccgatgtt ccagcaattc
     8761 aggctgttcg cacagcttgc gatgcaacga accaccggtt gccaccacca gttcttgtaa
     8821 accccggcag atggcgaaaa tggggatgcg cctttcgagt gcggcattaa ttatcgccat
     8881 gctcagaaga tcacgcccgg gatcggcgtc aggctcatcg ccgttttcac catataggtg
     8941 cggctgcaca ttgctgggac taccaggaag ataaatgcca tcgagtttcg gcaaaagttg
     9001 ttcaagtaac gacggttccg ccagcgcatg tggtagcgca attggcaagc cgcctgcgtg
     9061 gatgatggca ttcaggtact tttcttgcag agtctgggtc gcatgaccct taagcctgtt
     9121 cctgcacatt acgacaccga taaccggatt gttcattata tttttcatgc tcaatctcac
     9181 aaagtggact aaattatcgc cattactgcg ctttaaatga cctttatgtt caatattttt
     9241 tcaatctagc agtgggtttt catttttgca aactaaattt aacatttagc aaacatttag
     9301 tttgcataca gattcgaatg gtggtcatta tattttacgc tttgataacg agcggaaaac
     9361 aaaccaaagg cgaagaatca tggaaaccaa tatcgttgaa gtagagaact ttgttcagca
     9421 gccagaagag aggcggggta gcgcctttac gcaggaagtg aaacgctacc tggagcgcta
     9481 cccgaatacg caatatgttg atgttttact tactgatttg aatggttgct tccgtggaaa
     9541 acgtatccct gtttcaagcc tgaagaagtt ggagaaaggg tgttatttcc cggcctcggt
     9601 gtttgcaatg gatattctgg gcaacgtagt agaagaagcg ggtctgggtc agaaaatggg
     9661 cgagccggat cgtacttgtg ttcctgttct cggttcctta accccttctg ccgccgatcc
     9721 agagtttatc ggtcagatgc tcctgaccat ggtcgatgaa gatggcgctc cctttgacgt
     9781 tgagccgcgg aacgttctta accgcctgtg gcagcagcta cgccagcgcg gattgttccc
     9841 ggttgtagcg gtagagctgg agttctattt actggatcgt cagcgcgacg ctgaagggta
     9901 tctgcaaccg ccctgcgcgc ccggcaccga taaccgcaat acacaaagcc aggtttactc
     9961 cgttgataac ctcaaccact tcgccgacgt gctcaatgat attgatgaac tggcgcagtt
    10021 acagttaatt cccgccgacg gtgcggtcgc cgaagcctcg ccgggtcagt ttgaaatcaa
    10081 cctttaccat accgataacg tgctggaagc ctgcgatgat gcgctggcgc taaaactcct
    10141 ggtgcgtctg atggcagaaa agcataagat gcactccact tttatggcga agccgtatga
    10201 agagcacgcg ggcagcggga tgcatatcca tatcagtatg caaaataatc gtggcgagaa
    10261 tgtgctttct gacacagaag gcgaagattc gccgctgctg aaaaagatgc tcgccgggat
    10321 gattgacctg atgccgtcgt cgatggcgtt gctggcacca aacgtgaact cgtatcgccg
    10381 cttccagccg ggaatgtatg tgccgacgca ggcgtcgtgg ggccataaca accgcaccgt
    10441 cgccctgcgt attccgtgcg gcgaccgtca taatcaccgc gtggaatatc gcgtggcggg
    10501 tgccgatgcc aacccatatc tggtgatggc agcgattttt gccggtattt tgcatggcct
    10561 tgataacgag ctgccgttgc aggaagaagt cgaaggcaac gggctggaac aggaaggctt
    10621 acccttcccg attcgccaga gcgatgccct gggtgagttt atcgagaatg atcacctgcg
    10681 ccgctattta ggcgaacggt tttgccatgt gtatcacgcc tgtaaaaatg atgagctgtt
    10741 gcagtttgag cgcctcatca cagaaacaga aattgagtgg atgttgaaaa acgcatagta
    10801 ccccttgcgg ggttgtttca tcgacctgag cggagccagg cctgacattt gctggtaatc
    10861 cagacatttc ccggatgtcg cgttatggag cgcaggcggc aacgggcttc cgttaacgat
    10921 taagactgtg acgaggaaat gagatgatgc cgatgattaa atccccgcat ggcgagggag
    10981 gctgcgtatg cgcccctcct gctactgact ggactccgtc ccctttgctt cccttgctaa
    11041 acaggtttga tttcaggtcg acacgaccgc aaaccttatt acgcaggggg aggcagcaat
    11101 tatggctatt aattcaccac tgaatattgc tgcgcaaccc ggcaaaaccc gtctgcgaaa
    11161 atcactgaaa ttgtggcagg tggtgatgat gggtctggcc tatctcacgc cgatgaccgt
    11221 atttgatact tttggtattg tctccggcat tagcgacggt cacgttccgg cgtcctattt
    11281 gctggcgctg gcgggcgtgc tgtttaccgc tatcagctac ggcaaactgg ttcgccagtt
    11341 tccggaggcc ggttcggcct atacctacgc gcaaaagtcg attaacccgc acgtcggatt
    11401 tatggtcggc tggtcatcgc tgctggatta tctctttttg ccgatgatca acgtcttgtt
    11461 ggcgaaaatc tatctctccg ccctcttccc ggaagtgccg ccgtgggtgt gggtagtaac
    11521 cttcgtcgcc attttaaccg ccgcgaatct gaagagcatc aacctggtcg ctaacttcaa
    11581 taccctgttt gtactggtgc aaatctccat catggtggtg tttatcttcc tggtagttca
    11641 gggactgcat aaaggagaag gcgttggcac cgtctggtca cttcagccgt ttatcagcga
    11701 gaacgcgcac ctgatcccga ttattaccgg ggcgacgatt gtctgtttct ccttcctcgg
    11761 tttcgatgcg gtgaccacgc tttcggaaga gacgccggac gccgcacgcg tgatcccgaa
    11821 agccatcttc ctgacggcgg tctacggtgg cgttatcttt atcgcggcgt cgttctttat
    11881 gcagctgttc tttcccgata tcagccgctt taaagatccg gatgccgcac tgcctgaaat
    11941 tgcgctgtac gtcggcggca agctcttcca gtcgattttc ctctgcacca cgtttgtgaa
    12001 cacgttagcc tctggcctgg cttcacatgc cagcgtgtcg cgtctgctgt atgtgatggg
    12061 gcgtgacaat gtgtttccgg agcgcgtgtt tggctatgtg cacccaaaat ggcggactcc
    12121 ggcactcaac gtcattatgg tcgggattgt cgcgttgtcg gcgctgttct tcgatttagt
    12181 taccgccaca gcgttaatta acttcggtgc tctggtggcg tttaccttcg tgaatctgtc
    12241 ggtgtttaat catttctggc ggcgtaaagg aatgaataaa agctggaagg atcacttcca
    12301 ctatttgctg atgccgctgg ttggcgcgct gacggtgggt gtgctgtggg ttaacctcga
    12361 gtcaacgtca ctgacactcg gtctggtatg ggcttcgtta ggcggcgcat atttatggta
    12421 tctgatccgc cgctatcgca aagtgccgtt gtatgatggt gacagaacgc cggtgagtga
    12481 aacgtaatag tcggatgcgc ggcgcattat cctgccggag ggtaatgcac cgcgccatta
    12541 actataaaaa gatgtatgac ccggatcacg agtgctatca tccgccctta tgcttagcgc
    12601 attaacccca ggggcaaata atgaaccacg atatcccgct aaaatatttt gatattgccg
    12661 atgagtacgc gaccgaatgc gcagagcctg tcgcagacgc tgaacgcaca ccgctggccc
    12721 actacttcca gttactgctc acccgtttga tgaacaatga agagatcagc gaagaagccc
    12781 aacatgaaat ggctgctgaa gcggggatta atcccgtacg cattgatgag atcgcagagt
    12841 ttttgaatca atggggtaat gagtaattat ttaattgcag acgatcggtc agtatcgttg
    12901 tccggataaa gcgttcacgc cgcatccggc taacaatgac acgcactgca gcgtgcttta
    12961 atgggtttct cacgatcgta ccgctctgct cctcgctgag ttgttctgtg agttatagtt
    13021 atagaggaag cacgggtacg aatgttgaac agatggcgac gccacctgtt caacgaccaa
    13081 ccacacggcc ctcattacga cccctgaaca caaacggtaa tggcaaatgt cattaaatat
    13141 tagtaagata gcctctttac ataaaatccc ttaatattat gcgccaggta ttatcgtctc
    13201 ttttggtgat tgctggactt gtgagtggtc aggcaatcgc cgcgcctgaa tcccccccgc
    13261 atgctgatat ccgcgacagc ggttttgtct attgcgtcag cgggcaagtc aacaccttta
    13321 acccatccaa agcgagcagt gggttaattg tcgataccct tgccgcccag ttttatgatc
    13381 gactgctgga tgtcgatccc tatacctatc gcctgatgcc ggaacttgcc gaaagctggg
    13441 aagtactcga caacggcgcg acctatcgct tccacctgcg tcgcgatgtt ccgtttcaaa
    13501 aaaccgactg gtttactccc actcgtaaaa tgaatgccga cgatgtggtg tttaccttcc
    13561 agcgaatttt tgaccgcaac aacccgtggc ataacgtcaa cggcagcaac ttcccctact
    13621 tcgacagcct gaaatttgcc gataacgtga aaagcgtccg caaactggat aatcataccg
    13681 ttgagttccg actcgcccag ccggatgctt cttttttgtg gcacctcgca acccattatg
    13741 cttcggtcat gtcggcagaa tatgcccgga agttagagaa agaagatcgc caggagcaac
    13801 tcgaccgtca accggtcggc accggtccgt atcagttgtc ggaataccgc gccgggcaat
    13861 ttattcgcct acaacgtcat gatgacttct ggcgcggtaa accgttaatg ccgcaggtag
    13921 tggtggattt aggctccggc ggcaccggac gtctgtcgaa actcctgacc ggggaatgcg
    13981 acgttctggc ctggcctgct gccagccagc tatccatttt gcgtgacgac ccgcgcttgc
    14041 gtttaacgct gcgtcctggg atgaacgtcg cctatctggc atttaacacc gccaaaccgc
    14101 cgctaaataa tcccgctgtc cgccatgcac tggcactggc gattaataac cagcgcctga
    14161 tgcaatccat ctattatggt acggctgaaa cggcggcctc tattttaccg cgcgcctcgt
    14221 gggcctatga caacgaggct aaaattactg aatacaatcc ggcgaaatcg cgcgaacagt
    14281 tgaaggcgtt ggggctggaa aacttaacgc tgaaactgtg ggtgcccaca cgttcgcagg
    14341 cgtggaaccc cagtccactg aaaactgccg aactgattca ggcggatatg gcgcaggttg
    14401 gcgtaaaagt ggtgattgtg ccggtagaag gtcgctttca ggaggcacgg ttgatggata
    14461 tgagccatga tctgacgtta tccggttggg cgacggacag taacgacccg gacagtttct
    14521 tccgtccttt actgagctgc gcggcaattc attcacagac caacctcgcc cactggtgcg
    14581 atccgaaatt cgacagcgtg ttgcgtaagg cgctctcctc gcagcagctg gcggcgcgta
    14641 ttgaagccta tgacgaagcg cagagtattc tggcgcagga attgcccatt ttgccgctgg
    14701 cgtcgtcatt gcgtttgcag gcctatcggt acgatatcaa aggtctggta cttagcccgt
    14761 ttggtaacgc ctcctttgct ggggtgtatc gcgagaaaca ggatgaggtg aaaaaaccat
    14821 gattatcttc accttacgcc gcattttgtt attgattgtc accttgttcc tgctgacctt
    14881 cgttggcttc agcttgagct atttcacccc tcatgcgccg ttacaaggcg cgtcactgtg
    14941 gaatgcctgg gtgttctggt ttaacggcct gatccactgg gattttggtg tttccagcat
    15001 taacggtcaa ccgattgccg aacagttaaa agaggtcttc ccggcgacga tggaactgtg
    15061 catcctcgcc ttcggttttg cgctgattgt tggcatcccc gtggggatga ttgccgggat
    15121 tacgcgccat aagtggcagg ataacttgat caacgccatc gccttactgg gcttttcaat
    15181 cccggtgttc tggctggcgc ttctgttgac gctgttttgt tcactcacgc taggttggtt
    15241 gcccgtttca gggcgtttcg atctgctcta cgaagtgaaa ccgattaccg gttttgcgtt
    15301 gattgatgcc tggctttcgg actcaccatg gcgggatgaa atgatcatga gcgcaatccg
    15361 ccatatgata ttgcccgtga tcaccctgtc ggttgcgcca acaactgaag tgatccgcct
    15421 gatgcgtatc agcactatcg aagtgtatga ccagaactat gtaaaagcgg cggcgacccg
    15481 cggtttgtcg cgctttacta ttttgcgtcg ccacgttctg cataacgcgt taccgccggt
    15541 tattcctcgc ctgggcttac agttttccac catgctgacg ctggcgatga tcaccgaaat
    15601 ggtttttagc tggccgggtt tagggcgctg gttaattaac gccatccgcc agcaggatta
    15661 cgcagccatt tccgccggag tgatggtgtg tggctcactg gtcattattg ttaacgtgat
    15721 ttctgatatt ttgggtgcca tggctaaccc tctgaaacat aaggaatggt atgccttacg
    15781 atagcgtata cagcgaaaag cgcccgccgg gcacgctgcg taccgcctgg cgcaaatttt
    15841 atagtgacgc ctctgcgatg gtcggcctgt acggctgcgc ggggctggct gtactgtgta
    15901 tttttggcgg ctggtttgcg ccttacggca tcgaccagca atttctcggt tatcaattat
    15961 tgccgccatc atggtcgcgc tatggcgaag tttctttctt cctggggact gacgacctcg
    16021 gacgcgatgt tttaagccgt ttactgagcg gagctgcgcc aaccgtgggt ggcgcatttg
    16081 tggtgacgct tgccgcgacg atctgtggcc tagtactggg caccttcgcc ggggcgacac
    16141 acggcctccg ctcagcggtg cttaaccata ttctggatac cttactggca atcccttcgc
    16201 tgctgttggc aattatcgtt gtagcgtttg ccggaccgag tttgtcccac gctatgcttg
    16261 ctgtctggct ggcattgctg ccgcgtatgg tacgttcgat ttacagcatg gtgcatgacg
    16321 aactggaaaa agagtacgtt atcgccgccc gtctggatgg cgcatcaacg ctgaatattc
    16381 tctggtttgc tgtgatgcca aacatcaccg ccgggctggt caccgagatc acccgcgcac
    16441 tgtcgatggc aattctcgat atcgccgcgc tgggctttct cgatctcggc gcacaactcc
    16501 cctcgcctga atggggagcg atgctcggtg atgcgctgga actgatttat gtcgccccgt
    16561 ggactgtcat gctgccaggt gcggcaatta tgattagcgt gttgttggtt aacctccttg
    16621 gcgatggcgt tcgtcgtgcg attattgcgg gggtggaata atgccattac tcgatattcg
    16681 taacctgacc attgaattta aaaccggtga tgagtgggtt aaagccgtcg accgggtaag
    16741 tatgacctta accgaaggtg aaatccgcgg tcttgttggt gaatccggtt ccggcaagag
    16801 tttgattgcg aaagcgattt gtggggtgaa taaagataac tggcgtgtta ctgctgaccg
    16861 tatgcgtttt gatgatatcg atttgctgcg tctctccgca cgcgaacggc gcaaactggt
    16921 tggccataac gtgtcgatga ttttccagga accgcagtcg tgtcttgacc cttcagaacg
    16981 tgtgggtcgc cagttgatgc aaaacatccc agcctggacc tataaaggcc gttggtggca
    17041 gcgttttggc tggcgcaaac gccgtgccat tgaactgctg caccgcgtgg ggattaaaga
    17101 tcacaaagat gcgatgcgca gttttcccta tgagttgacc gaaggtgaat gtcagaaagt
    17161 gatgatagcc attgcactgg cgaatcaacc gcgtctgctg attgctgacg aaccgaccaa
    17221 ctcaatggag ccaacaaccc aggcgcaaat ctttcgcctg ctgacgcgtc tcaaccaaaa
    17281 cagcaatacc actattttgc ttatcagcca tgacttacaa atgcttagcc aatgggcgga
    17341 taaaattaac gtgctttact gcggtcaaac agtggaaacc gcgccgagta aggagttggt
    17401 gacgatgcca catcatcctt atacccaggc gctgatccgc gcgataccag acttcggcag
    17461 cgcgatgccg cataaaagtc gcctcaatac gctgcccggc gctatcccac tgctggaaca
    17521 gttaccgatt ggttgtcgtc tggggccacg ttgcccgtat gcacaacgag aatgcattgt
    17581 gacgccacgt ttgacggggg cgaaaaatca tctctatgcc tgtcatttcc cgctgaacat
    17641 ggagaaagag tgagatgatc gaaacgctgc ttgaagtgcg taatttaagt aaaacgttcc
    17701 gctaccggac cggctggttt cgccgtcaga ccgtagaagc ggtaaaaccc ttgagcttta
    17761 cgctacgtga aggccagaca ctggcgatta ttggcgagaa tggttcgggt aaatccacgc
    17821 tggcaaaaat gctggcggga atgatagagc ctaccagcgg cgagttattg attgacgatc
    17881 atccactgca ttttggcgat tattccttcc gcagtcagcg tattcgcatg atttttcagg
    17941 atccttcgac ctcgttgaat ccccgtcaac gtatctcgca aattctggat tttccactgc
    18001 gcctgaacac cgacctggaa ccggaacagc gtcgcaaaca gattattgaa acgatgcgta
    18061 tggttgggct gctaccggat cacgtcagtt actatccgca tatgttggca cccggacaaa
    18121 aacagcgtct gggtctggcg cgcgcgctga tattgcgccc aaaagtcatt attgccgatg
    18181 aagcactcgc ttcactggat atgtcgatgc gttcgcagtt gattaatctg atgctggagt
    18241 tacaggaaaa acagggcatt tcgtatattt atgttaccca gcatatcgga atgatgaagc
    18301 acattagcga ccaggtgctg gtgatgcatc agggcgaggt tgtcgagcga ggcagcaccg
    18361 cggatgtgct ggcatcgccg ctgcatgaac tcaccaaacg gctgattgcc gggcactttg
    18421 gcgaagcatt gacagcggat gcgtggcgta aagatcgcta attttctgaa tcctccttcc
    18481 agattatcca cctcattctg acaagagatt aaatcttgtc agaatgaggt gatggataaa
    18541 ataaaatcaa atgcgcgtga tttaagacgc aatctcactt tgcaggaacg aaagctctgg
    18601 cgatatcttc gcagccgacg ttttggtgat ttcaaatttc gccgtcaaca tccggtgggg
    18661 agctacattc tcgattttgc ttgctgctcg gcgcgcgtag tcgttgagct ggatggtggg
    18721 cagcatgatt tggcagttgc ctatgatacc aggcgcacta gctggcttga gtcgcagggc
    18781 tggaccgtgc tgcgtttctg gaataacgag attgattgta atgaggaggc ggtgctggag
    18841 attattctgc aggaactgaa ccgccgatca ccctcaccct gaaagggcga gggggccgac
    18901 cgagccgaat agctgttgtg gtgaaaacat ggagacagtg ctggagaata ttcggcaagg
    18961 tctgaaccat cccagccatc gccataaaag ggttaggggc tgtatgagcc tgtttgttgc
    19021 tggggtaaca atatttgcac aatatggtcc cctcgcccct ctggggagag ggttagtgta
    19081 aggggaaaag cgcccaccct gccgcagcct gctccggtcg gacctagcaa ctatagctac
    19141 tcacagccag gttgattata ataaccattt atctgttcgt actgtttact aaaacgacga
    19201 atcgcctgat tttcaggcac aacaagcatc aacaataagg attaaagcta tgggttttct
    19261 ttccggtaag cgcattctgg taaccggtgt tgccagcaaa ctatccatcg cctacggtat
    19321 cgctcaggcg atgcaccgcg aaggagctga actggcattc acctaccaga acgacaaact
    19381 gaaaggccgc gtagaagaat ttgccgctca attgggttct gacatcgttc tgcagtgcga
    19441 tgttgcagaa gatgccagca tcgacaccat gttcgctgaa ctggggaaag tttggccgaa
    19501 atttgacggt ttcgtacact ctattggttt tgcacctggc gatcagctgg atggtgacta
    19561 tgttaacgcc gttacccgtg aaggcttcaa aattgcccac gacatcagct cctacagctt
    19621 cgttgcaatg gcaaaagctt gccgctccat gctgaatccg ggttctgccc tgctgaccct
    19681 ttcctacctt ggcgctgagc gcgctatccc gaactacaac gttatgggtc tggcaaaagc
    19741 gtctctggaa gcgaacgtgc gctatatggc gaacgcgatg ggtccggaag gtgtgcgtgt
    19801 taacgccatc tctgctggtc cgatccgtac tctagcggct tccggtatca aagacttccg
    19861 caaaatgctg gctcattgcg aagccgttac cccgattcgc cgtaccgtta ctattgaaga
    19921 tgtgggtaac tccgcggcat tcctgtgctc cgatctctct gccggtatct ccggtgaagt
    19981 ggtccacgtt gacggcggtt tcagcatcgc tgcaatgaac gaactcgaat tgaaataatc
    20041 attctgttgg taaatattgg gcggcgttct gccgcccgtt atctctgtta tacctttctg
    20101 atatttgtta tcgccgatcc gtctttctcc ccttcccgcc ttgcgtcagg ataacgattt
    20161 cctttacgac caaggagcgc ccatggaaca acgccacatc accggcaaaa gccactggta
    20221 tcatgaaacg caatccagta ctgcggagta tgacgttctg cctctggtcc cggaagccgc
    20281 aaaggtcagc gatccctttc tgctcgacgt gatccttgat gaagaaacgc tagctccctt
    20341 cctttcatgg ctggtccctg cgcgcgttct tgcagtggaa ttgttccctg accagcttac
    20401 cgtgacccgt tcacagacct tcaccgctta tgaacgcttg tctacggccc tgacggttgc
    20461 tcaggtttgc ggcgtccagc ggttatgtaa ctactattcg gcgcgactta cgccgctccc
    20521 cgggcctgat tcctctaggg aaagcaatca taggttggca caaatcacgc aatatgcccg
    20581 ccaactggct agctcgcctt ctattatcga caaccgatcg cgccagcatc tgaatgacgt
    20641 cggtcttact gccagggact gtgtaatcat taaccaaatc attggtttta ttggctttca
    20701 ggcgcggacc attgcgacat ttcaggctta tctcgggcac ccggtacgct ggttacccgg
    20761 tctggaaata caaaactacg ccgacgcgtc actgtttgct gatgaatcaa tacgctggcg
    20821 aagcagctat gaggtggaaa aactacctga agagtacaca aaaagttcaa cagcagaact
    20881 ttgccaactg gccgaaacac tctctctcca ccctatttca ctttcccttc tcgaaaggtt
    20941 gttaaacagt acacgggtta atacacagcc ggataataag cttgcggcgt tgttatgcgc
    21001 gcggataaat ggcagtcctg cttgttttgc cgcctgtatg gattcatcaa atgaatataa
    21061 aaaaatcagc ccccttctgc gcaagggcga aaatgaaatt aaccaatggg ctgaccgtca
    21121 ttctgttgag cacgctaccg ttcaggcgat acaatggctg acccgagcac ccgatcgctt
    21181 tagcaccgcc cagttcagcc ctttactcga acacgaacaa tcatcaacgc agattattaa
    21241 tctgctggta tggagcgggc tgtgtggctg gataaatcgc ttaaaaatcg cgttgggtga
    21301 gacatattaa ccttgccgcg tcagacagat tcgcgtaaaa ctgtcagccg ctctaatggc
    21361 caccaaaata gacaattatg tttcaggaca acccgctgct agcgcagctt aaacagcaac
    21421 tgcattccca gacgccacgc gctgaagggg tggtaaaagc cacagaaaaa ggctttggct
    21481 tcctggaagt cgacgcgcaa aaaagttatt tcattccgcc gccgcagatg aaaaaagtca
    21541 tgcatggcga ccgaattatc gcggtgatcc acagtgaaaa agaacgtgaa tccgcagagc
    21601 cagaagaact ggttgaaccg ttcctgactc gtttcgtggg taaagttcag ggcaaaaatg
    21661 accgtctggc catcgttcct gatcatccac tcttaaaaga cgccattcct tgccgcgcag
    21721 cccgtggcct gaaccacgag tttaaagaag gcgactgggc ggttgccgaa atgcgccgtc
    21781 atccgctgaa aggcgatcgt tctttctatg ctgaattgac acaatacatc acttttggtg
    21841 acgaccactt tgtaccgtgg tgggttacac ttgcgcgcca taatctggaa aaagaagcac
    21901 cagacggcgt cgctaccgaa atgctcgatg aaggtctggt tcgtgaagat ctgaccgcgc
    21961 tggattttgt caccatcgac agtgccagca cagaagatat ggatgacgcc cttttcgcta
    22021 aggcgttgcc ggatgacaaa cttcagctga ttgtggcgat tgccgatcca accgcgtgga
    22081 ttgctgaagg cagcaagctg gacaaagccg cgaaaattcg cgcattcacc aactatctgc
    22141 ctggcttcaa catccctatg ctgcctcgcg agctttctga cgatctctgc tcactgcgcg
    22201 ccaatgaagt ccgcccggta ctggcatgcc gcatgacgct ctccgctgat ggcgccattg
    22261 aagataatat cgaattcttt gccgccacca tcgaatccaa agcaaagctg gtgtatgacc
    22321 aggtttctga ctggctggaa aataccggtg actggcagcc tgaaagtgaa gcaattgccg
    22381 aacaagtccg tttgctagcg caaatttgcc aacgccgcgg cgagtggcgt cataaccacg
    22441 cactggtgtt taaagatcgc ccggattacc gctttattct cggtgaaaaa ggtgaagtgc
    22501 tggatatcgt cgccgagcct cgtcgcattg ccaaccgtat cgtcgaagaa gcgatgattg
    22561 ccgctaacat ttgtgcggcc cgcgtactgc gcgataagct cggttttggc atctataacg
    22621 tgcatatggg ctttgatccg gcgaatgccg acgcgctggc agcgttgctg aaaacgcacg
    22681 gtctgcatgt cgatgccgaa gaagtgctca cgctggacgg tttctgcaaa ctgcgtcgtg
    22741 aactggacgc gcaaccaact ggtttcctcg acagccgcat tcgtcgcttc cagtcatttg
    22801 ctgaaattag cactgaaccc ggtcctcact ttggcctcgg tctggaagca tacgccacct
    22861 ggacttcgcc gatccgtaaa tatggcgaca tgatcaacca ccgtctgctg aaagcggtta
    22921 tcaaaggcga aactgcgacg cgcccacagg atgaaatcac tgtccaaatg gccgagcgtc
    22981 gccgtctcaa ccggatggca gaacgtgatg ttggtgactg gttatacgca cgcttcctga
    23041 aagacaaagc cgggaccgac acccgtttcg cagcggaaat tgtcgatatc agccgtggcg
    23101 gcatgcgtgt gcgtttggtt gataacggcg ctatcgcctt tattccggca cctttcttac
    23161 acgctatgcg cgatgaactg gtttgcagcc aggaaaacgg cactgtacaa attaaaggtg
    23221 aaacggttta caaagtaact gacgttattg acgtcaccat tgccgaagtc cgcatggaaa
    23281 cccgcagcat tattgcgcgc ccggtcgcgt aatctccttt cacggcctat tcctcatgga
    23341 tgggccgttt atttccccgc tctaccttcg ttatatccag gcagattttt taactgactt
    23401 tcgtttgaaa actggcgttt ttccaaaact ggattacaaa taaatatata aaaaaacaat
    23461 gcgtttagcc gccgttaatt tcatgtaagt ca
//
LOCUS       DAJTMC010000053        23235 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.053, whole genome shotgun sequence.
ACCESSION   DAJTMC010000053 DAJTMC010000000
VERSION     DAJTMC010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23235
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.053"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(<1..562)
                     /locus_tag="OO130_001837"
     CDS             complement(<1..562)
                     /locus_tag="OO130_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5365278.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGG"
     gene            complement(781..1758)
                     /gene="epmA"
                     /locus_tag="OO130_001838"
     CDS             complement(781..1758)
                     /gene="epmA"
                     /locus_tag="OO130_001838"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HCR5365279.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            2083..3891
                     /gene="frdA"
                     /locus_tag="OO130_001839"
     CDS             2083..3891
                     /gene="frdA"
                     /locus_tag="OO130_001839"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HCR5365280.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRMAGEQATERAATAGNGNEEAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            3884..4618
                     /gene="frdB"
                     /locus_tag="OO130_001840"
     CDS             3884..4618
                     /gene="frdB"
                     /locus_tag="OO130_001840"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HCR5365281.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4629..5024
                     /gene="frdC"
                     /locus_tag="OO130_001841"
     CDS             4629..5024
                     /gene="frdC"
                     /locus_tag="OO130_001841"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HCR5365282.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPDAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            5035..5394
                     /gene="frdD"
                     /locus_tag="OO130_001842"
     CDS             5035..5394
                     /gene="frdD"
                     /locus_tag="OO130_001842"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HCR5365283.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            5457..6590
                     /locus_tag="OO130_001843"
     CDS             5457..6590
                     /locus_tag="OO130_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HCR5365284.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQMPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLTPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            6679..7212
                     /gene="blc"
                     /locus_tag="OO130_001844"
     CDS             6679..7212
                     /gene="blc"
                     /locus_tag="OO130_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HCR5365285.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDGGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(7209..7526)
                     /gene="sugE"
                     /locus_tag="OO130_001845"
     CDS             complement(7209..7526)
                     /gene="sugE"
                     /locus_tag="OO130_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HCR5365286.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7708..7854)
                     /gene="ecnB"
                     /locus_tag="OO130_001846"
     CDS             complement(7708..7854)
                     /gene="ecnB"
                     /locus_tag="OO130_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HCR5365287.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7965..8090)
                     /gene="ecnA"
                     /locus_tag="OO130_001847"
     CDS             complement(7965..8090)
                     /gene="ecnA"
                     /locus_tag="OO130_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HCR5365288.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(8142..8708)
                     /gene="efp"
                     /locus_tag="OO130_001848"
     CDS             complement(8142..8708)
                     /gene="efp"
                     /locus_tag="OO130_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCR5365289.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8750..9799
                     /gene="epmB"
                     /locus_tag="OO130_001849"
     CDS             8750..9799
                     /gene="epmB"
                     /locus_tag="OO130_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000940527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HCR5365290.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITDALVERFSHSTL
                     QILLVNHINHANEIDETFRQAMAKLRRVGVTLLNQSVLLRDVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQLLIKQGS"
     gene            10173..11042
                     /locus_tag="OO130_001850"
     CDS             10173..11042
                     /locus_tag="OO130_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HCR5365291.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQENGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEVEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(11245..11598)
                     /locus_tag="OO130_001851"
     CDS             complement(11245..11598)
                     /locus_tag="OO130_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HCR5365292.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(11736..13382)
                     /gene="groL"
                     /locus_tag="OO130_001852"
     CDS             complement(11736..13382)
                     /gene="groL"
                     /locus_tag="OO130_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HCR5365293.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(13426..13719)
                     /locus_tag="OO130_001853"
     CDS             complement(13426..13719)
                     /locus_tag="OO130_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HCR5365294.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            13995..15251
                     /gene="yjeH"
                     /locus_tag="OO130_001854"
     CDS             13995..15251
                     /gene="yjeH"
                     /locus_tag="OO130_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HCR5365295.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAAQVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPCAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRSASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPANIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEKMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIVLAGLWLLLPKRKTPENGIIT"
     gene            complement(15267..15743)
                     /gene="fxsA"
                     /locus_tag="OO130_001855"
     CDS             complement(15267..15743)
                     /gene="fxsA"
                     /locus_tag="OO130_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HCR5365296.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDERDRLDHKDDRQD"
     gene            16080..17516
                     /gene="aspA"
                     /locus_tag="OO130_001856"
     CDS             16080..17516
                     /gene="aspA"
                     /locus_tag="OO130_001856"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HCR5365297.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            17634..18935
                     /gene="dcuA"
                     /locus_tag="OO130_001857"
     CDS             17634..18935
                     /gene="dcuA"
                     /locus_tag="OO130_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HCR5365298.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            19051..19389
                     /gene="cutA"
                     /locus_tag="OO130_001858"
     CDS             19051..19389
                     /gene="cutA"
                     /locus_tag="OO130_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HCR5365299.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            19365..21062
                     /gene="dsbD"
                     /locus_tag="OO130_001859"
     CDS             19365..21062
                     /gene="dsbD"
                     /locus_tag="OO130_001859"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HCR5365300.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFTFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVSQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNHALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NITANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            21099..21674
                     /locus_tag="OO130_001860"
     CDS             21099..21674
                     /locus_tag="OO130_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR5365301.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKQLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            21781..21856
                     /locus_tag="OO130_001861"
     tRNA            21781..21856
                     /locus_tag="OO130_001861"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21814..21816,aa:Phe,seq:gaa)
     gene            22054..23025
                     /locus_tag="OO130_001862"
                     /pseudo
     CDS             22054..23025
                     /locus_tag="OO130_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
BASE COUNT         5804 a         5992 c         5747 g         5692 t
ORIGIN      
        1 gcccaccatt taataacaaa atagtaatgc ttactaacag caatactaaa ttcagacaca
       61 ttactgcaat accgcccacc gcagtaatgc gggcaatttt attaatcccc tttgaagcaa
      121 cgacggtgac cagaatcatc catgctactg ccagcagacc aaccacctgc gtaggctcca
      181 gtccggcaat acgccagtgc tgggtcatgt cgctaccata gaggaatgtt gagaacggta
      241 cccaaacttt cgcggaggta ctcaccatcc agatgatata agaggaaaac cacataaacg
      301 taccaatgaa ggcaaaacgt ggtccgacac tattattcat ccaggaatag ataccacctt
      361 cttctttgcg ataagcagct cccatttcag ccatcattaa ggcgaatgga ataaagaata
      421 acaatgcaga aaatatataa aagggaatcg cactataacc cattaagtaa taagccgatg
      481 ggctattggc aaatccaaat acggaagtaa agatcatcaa tatgagtcct atcagactca
      541 tcttttttat cgtgtgaggc atgaaaccat ccttcatttc agcgcgtgta taacacgtgc
      601 ccactgaact gcactgcgca acattgagca ggcagcgtaa aaaccaatac agaattgata
      661 cccggatgat atcaaatatc cagtcttaaa ttgtggcctt taacggaaaa atgaaaacat
      721 atgctacaga atggcgctta tcacgccatt cttcgctgtt aattcagtaa tttttcagaa
      781 ttatgcccgg tcaacgctaa aggcgatgac ttcagccagt gtctccgcgc ccagcgccaa
      841 catcaccaga cgatcaacac ctaatgccac gccggaacag tcaggcatac cgactttcaa
      901 ggcttcaatc agattctggt caatggggtg ctgcggcaaa ccgcgcgccg cgcgcttacg
      961 gttatcttgt tcaaagcgtt gttgctgctc acgggcatcc gttaattcat ggaaaccatt
     1021 cgccagctca atacctttat aataaacctc aaagcgttca gcgacccgat gatcttcggt
     1081 actgatttgc gccagtgatg cctggctggc tggaaagtgg tacacaaagg tcggtttttc
     1141 tttgccaata tttggctcta cgccaaaggt aaacagcaat tgtagcaacg tgtcgcggtc
     1201 ttcttcggta tcagcaacgt tactcaaatc cagtttcgct gcgacttccc gcagttgcgt
     1261 tttgtcggca gagagcgggt caatttccag ataacgcaag aaagcttgtt gataagaaag
     1321 gctttctgct gccgggcagt ccagcacctg ttgtaagaga tcgtccacct cgttcatcaa
     1381 ccggtacata tcatagtgcg gtcgatacca ctcaagcata gtgaactcag ggttgtgata
     1441 acgccccatc tcttcattac ggaagctgcg gcacagctgg aataccggcc cacaaccggc
     1501 aaccagcagg cgtttcatat ggtattccgg gctggtcatt aaccagagat tcatcccctg
     1561 cgaatgcccg gggccaacga aacgtgtctc aaacgggacc aaatgaatat cggttaccgt
     1621 cgcctggctc atacaaggcg tctccacctc cagcactcca cgatcagcaa agaaacgacg
     1681 gatctccgcc ataatcgccg cgcgttttaa taagttagga atggatgcgc tcggctgcca
     1741 ggatgccgtt tcgctcatag ttaaatctcc agtttttgac aagggcacga agtctactcg
     1801 caacgcgacg gcgagacaaa ttttacgcag gaatcaaaca gcggtgggca gtgactaaaa
     1861 aaagcacgat ctgatggttt agtaattaaa ttaatcatct tcagtgataa tttagccctc
     1921 ttgcgcacta aaaaaatcga tctcgtcaaa tttcagactt atccatcaga ctatactgtt
     1981 gtacctataa aggagcagtg gaatagcgtt cgcagaccgt aactttcagg cacttaccct
     2041 gaagtacggg gctgtgggat aaaaacaatc tggaggaatg tcgtgcaaac ctttcaagcc
     2101 gatcttgcca ttgtaggcgc cggtggcgcg ggattacgtg ctgcaattgc tgccgcgcag
     2161 gcaaatccaa atgcaaaaat cgcactaatc tcaaaagtat acccgatgcg tagccatacc
     2221 gttgctgcag aagggggctc cgccgctgtc gcgcaggatc atgacagctt cgaatatcac
     2281 tttcacgata cagtagcggg tggcgactgg ttgtgtgagc aggatgtcgt ggattatttc
     2341 gtccaccact gcccaaccga aatgacccaa ctggaactgt gggggtgccc atggagccgt
     2401 cgcccggatg gtagcgtcaa cgtacgtcgc ttcggcggca tgaaaatcga gcgcacctgg
     2461 ttcgccgccg ataagaccgg cttccatatg ctgcacacgc tgttccagac ctctctgcaa
     2521 ttcccgcaga tccagcgttt tgacgaacat ttcgtgctgg atattctggt tgatgatggt
     2581 catgttcgcg gcctggtagc aatgaacatg atggaaggca cgctggtgca gatccgtgct
     2641 aacgcggtcg ttatggctac tggcggtgcg ggtcgcgttt atcgttacaa caccaacggc
     2701 ggcatcgtta ccggtgacgg tatgggtatg gcgctaagcc acggcgttcc gctgcgtgac
     2761 atggaattcg ttcagtatca cccaaccggt ctgccaggtt ccggtatcct gatgaccgaa
     2821 ggctgccgtg gtgaaggcgg tattctggtc aacaaaaatg gctaccgtta tctgcaagat
     2881 tacggcatgg gcccggaaac tccgctgggc gagccgaaaa acaaatatat ggaactgggt
     2941 ccacgcgaca aagtttctca ggccttctgg cacgaatggc gtaaaggcaa caccatctcc
     3001 acgccgcgtg gcgatgtggt ttatctcgac ttgcgtcacc tcggcgagaa aaaactgcat
     3061 gaacgtctgc cgttcatctg cgaactggcg aaagcgtacg ttggcgtcga tccggttaaa
     3121 gaaccgattc cggtacgtcc gaccgcacac tacaccatgg gcggtatcga aaccgatcag
     3181 aactgtgaaa cccgcattaa aggtctgttc gccgtgggtg aatgttcctc tgttggtctg
     3241 cacggtgcaa accgtctggg ctccaactcc ctggcggaac tggtggtctt cggccgtatg
     3301 gccggtgaac aagcgacaga gcgtgcagca actgccggta atggcaacga agaggcaatt
     3361 gaagcgcagg cagctggcgt tgaacaacgt ctgaaagatc tggttaacca ggatggcggc
     3421 gaaaactggg cgaagatccg cgacgaaatg ggcctagcaa tggaagaagg ttgcggtatc
     3481 taccgtacgc cggaactgat gcagaaaacc atcgacaagc tggcagagct gcaggaacgc
     3541 ttcaagcgcg tgcgcatcac cgacacctcc agcgtgttca acaccgacct gctctacacc
     3601 attgaactgg gccacggtct gaacgttgct gaatgtatgg cgcactccgc aatggcacgt
     3661 aaagagtccc gcggcgcaca ccagcgtctg gacgaaggtt gcaccgagcg tgacgacgtc
     3721 aacttcctca aacacaccct cgccttccgc gatgctgatg gcacgactcg cctggagtac
     3781 agcgacgtga agattactac gctgccgcca gctaaacgcg tttacggtgg cgaagcggat
     3841 gcagccgata aggcggaagc agccaataag aaggagaagg cgaatggctg agatgaaaaa
     3901 cctgaaaatt gaggtggtgc gctataaccc ggaagtcgat accgcaccgc atagcgcatt
     3961 ctatgaagtg ccttatgacg caactacctc attactggat gcgctgggct acatcaaaga
     4021 caacctggca ccggacctga gttaccgctg gtcctgccgt atggcgattt gtggttcctg
     4081 cggcatgatg gttaacaacg tgccaaaact ggcatgtaaa accttcctgc gtgattacac
     4141 cgacggtatg aaggttgaag cgttagctaa cttcccgatt gaacgcgatc tggtggtcga
     4201 tatgacccac ttcatcgaaa gtctggaagc gatcaaaccg tacatcatcg gcaactcccg
     4261 caccgcggat cagggtacta acatccagac cccggcgcag atggcaaagt atcaccagtt
     4321 ctccggttgc atcaactgtg gtctgtgcta cgccgcgtgc ccgcagtttg gcctgaaccc
     4381 agagttcatc ggtccggctg ccattacgct ggcgcatcgt tataacgaag atagccgcga
     4441 ccacggtaag aaggagcgta tggcgcagtt gaacagccag aacggcgtat ggagctgtac
     4501 tttcgtgggc tattgctccg aagtctgccc gaaacacgtc gatccggctg cggccattca
     4561 gcagggcaaa gtagaaagtt cgaaagactt tcttatcgcg accctgaaac cacgctaagg
     4621 agtgcaacat gacgactaaa cgtaaaccgt atgtacggcc aatgacgtcc acctggtgga
     4681 aaaaattgcc gttttatcgc ttttacatgc tgcgcgaagg cacggcggtt ccggctgtgt
     4741 ggttcagcat tgaactgatt ttcgggctgt ttgccctgaa aaatggcccg gacgcctggg
     4801 cgggattcgt cgacttttta caaaacccgg ttatcgtgat cattaacctg atcactctgg
     4861 cggcagctct gctgcacacc aaaacctggt ttgaactggc accgaaagcg gccaatatca
     4921 ttgtaaaaga cgaaaaaatg ggaccagagc caattatcaa aagtctctgg gcggtaactg
     4981 tggttgccac catcgtaatc ctgtttgttg ccctgtactg gtaaggagcc tgagatgatt
     5041 aatccaaatc caaagcgttc tgacgaaccg gtattctggg gcctcttcgg ggccggtggt
     5101 atgtggagcg caattattgc gccggtgatg atcctgctgg tgggtattct gctgccactg
     5161 gggctgtttc cgggtgatgc gctgagctac gagcgcgttc tggcgttcgc gcagagcttc
     5221 attggtcgcg tattcctgtt cctgatgatc gttctgccgc tgtggtgtgg tttacaccgt
     5281 atgcaccacg cgatgcacga tctgaaaatt cacgtgcctg cgggcaaatg ggttttctac
     5341 ggtctggctg ctatcctgac agttgtcacg ctgattggta tcgttacaat ctaacgtatc
     5401 gccaatgtaa atccggcccg cctatggcgg gccgttttgt atggaaacca gaccttatgt
     5461 tcaaaacgac gctctgcgcc ttattaatta ccgcctcttg ctccacattt gctgcccctc
     5521 aacaaatcaa cgatattgtg catcgcacaa ttaccccgct tatagagcaa caaaagatcc
     5581 ccggtatggc ggtggcggta atttatcagg gtaaacctta ttactttacc tggggctatg
     5641 cggacatcgc caaaaagcag cccgtcacac agcaaacgtt gtttgagtta ggttcggtca
     5701 gcaaaacatt tacgggcgtg cttggtggcg atgctattgc tcgaggggaa atcaagttaa
     5761 gcgatcccgc aacaaaatac tggcctgaac ttaccgctaa acagtggaat gggatcacac
     5821 tattacatct tgcgacctac accgctggcg gcctgccatt gcagatgccg gatgaagtga
     5881 aatcctcaag cgacttgctg cgcttctatc aaaactggca gcctgcatgg gctccaggaa
     5941 cacaacgtct gtatgccaac tccagtatcg gtttgttcgg cgcactggct gtgaagccgt
     6001 ctggtttgag ttttgagcag gcgatgcaaa ctcgtgtctt ccagccactc aaactcaacc
     6061 atacgtggat taatgtaccg cccgcagaag aaaagaatta cgcctgggga tatcgcgaag
     6121 gcaaggcagt tcatgtttcg ccaggggcgt tagatgctga agcttatggt gtgaagtcga
     6181 ccattgaaga tatggcccgc tgggtgcaaa gcaatttaac cccccttgat atcaatgaga
     6241 aaacgcttca acaagggata caactggcac aatctcgcta ctggcaaacc ggcgatatgt
     6301 atcagggcct gggctgggaa atgctggact ggccggtcaa tcctgacagc atcattaacg
     6361 gcagtgacaa taaaattgca ctggcagcac gcccggtaaa agcgattaag cccccaactc
     6421 ctgcagtacg cgcatcatgg gtacataaaa cgggggcgac cggcggattt ggtagctatg
     6481 tcgcgtttat tccagaaaaa gagctgggta tcgtgatgct ggctaacaaa aactatccca
     6541 atccagcgag agtcgccgcc gcctgtcaga ttctcaacgc tctacagtaa aatttcatcg
     6601 ggtccgaatt ttcggacctt ttctgcgctt ttccttgctg tcatctacac ttagaaaaaa
     6661 accagtaagg aaacatttat gcgcctgctc cctctcgttg ccgcagcgac agctgcattt
     6721 ctggtcgttg cctgcagttc tcctacgccg ccgcgtggcg tgacagtggt gaataatttc
     6781 gacgctaaac gctatctcgg tacctggtat gagattgccc gttttgatca ccgttttgaa
     6841 cgtggactgg aaaaagtcac cgcaacatac agtctgcgtg atggcggcgg cctgaatgtc
     6901 atcaataaag gctataaccc tgacagagaa atgtggcagc agagtgaagg gaaagcgtac
     6961 tttaccggcg caccaacacg cgctgcgctg aaagtgtcat tctttggtcc tttctatggc
     7021 ggttataacg tcattgcact tgatcgggaa taccgccatg cgctggtttg cggcccggac
     7081 cgcgactacc tgtggatact ctcccgcacg ccaaccattt ctgacgaagt gaaacaggag
     7141 atgctggcag tcgcgacccg ggaagggttt gatgtcagta agtttatttg ggtacagcag
     7201 cccggtagtt agtgagtgct gagtttcaga ccaataatcc ccaatacgat taacgccaga
     7261 ctcgccaggc gcatcgggtt agcggactca ccgagcagca caatgccggt gatagccgcg
     7321 ccgactgcgc caatacccgt ccacacggca taagccgtcc ctactggtaa cgatttcatc
     7381 gcccaggcaa gtagcgccat actgacaatc atcgccgtca cggtaataac actcggcgtc
     7441 aaacgactaa agccgtgggt atatttcagg ccaacggccc ataccacttc cagcagacca
     7501 gcaataacta agataatcca ggacatatca ggctccggag aattggggcc gtccccggtg
     7561 aaaagacgcg tttgcaggtc gtcctgcaaa gctgatggtt gaacggatat tttgccaggt
     7621 ggcaggggaa tttcaactat taccttagtg actatcgcag aaaaaacaaa cggcacgaca
     7681 cagctgtcgt accgttattg cttattgcta ttgctgcgct ttcgttgctg cgccagaaat
     7741 cgcgttaccg ccatcagaaa tgtcttcacc aacgccacgc gtggtgttgc aggcagttaa
     7801 tactgttgaa agcaccagaa cagaaaagat cgctgcaatt gtcttcttca ccataacgtc
     7861 ttccttttag gtaatgttat ttatgtttgc ctatagcaaa ttaataatag acaacatcgc
     7921 caaaaatgac ggaaatctga tgatttttcg gaagagaaga aaaattagct ggcagcgcga
     7981 gagatggagt tgccgagatg tttgatgtct tcgccgaaac cgcgagcggt gttacagccc
     8041 gtgagcagcg tactggcaag caaaacaaga acgataaggc gtttcatcat ctctgacctg
     8101 aataagtgat ggtgcagcct gcaggccgca ccacaaccgc attacttcac gcgagagacg
     8161 tattcaccag agcgggtatc cactttgatg acttcgccga tttgtacgaa cagcggaacc
     8221 ttaaccacag cgccagtaga cagggtagcc ggtttgccgc cagtacctgc ggtatcacct
     8281 ttcaggcccg gatcggtatc aacgatttcc agttcaacga agttcggcgg agtaacggag
     8341 atcggctgac cattccacag agttacgata cactctgcct gatccagcag ccatttagcg
     8401 ttatcgccaa ttgcttttgc atcagcagac agctgctcga aagtttcgtt gttcatgaag
     8461 tgccagaact caccgtcgtt gtacaggtaa gtcaggttca tatcgacaac atcagcgcct
     8521 tcagcggaat cagtagattt gaaggttttt tctacgcgag taccggtcag cagacgacgc
     8581 agtttaacgc gagcaaatgc ctggccttta cccggtttta cgaattcact cgcttcaacc
     8641 gcgtaaggtt cgccgtctaa catgatttta agaccagcac gaaaatcgtt gctatagtac
     8701 gttgccataa ggccctctga aatttgttaa ttggtagcta agccacaaaa tggcgcatat
     8761 tgtaacccta aataccccat ccagagaaga ttggttaacg caacttgccg atgttgtgac
     8821 cgatcctgat gaacttctgc gtcttttgaa tatagacgcg gacgaaaaac tgttggccgg
     8881 acgcagcgcc aaaaagctgt ttgccctgcg tgtgccccgc tcatttatcg atcgcatgga
     8941 gaaaggcaat ccggacgatc ctcttttgcg tcaggtactt acctcgcaag atgagtttgt
     9001 cgtcgcgtcc ggattctcca ccgacccact ggaagaacag cacagcgtag tgcctggttt
     9061 gttgcataaa taccacaacc gggcgctttt gctggtcaaa ggcggctgcg cggtaaattg
     9121 ccgctattgc ttccgtcgcc acttccccta tgccgaaaat cagggcaaca agcgtaactg
     9181 gcagactgca cttgagtatg ttgctgcgca tccggaactg gacgagatga ttttctccgg
     9241 cggcgatccg ctgatggcga aagatcacga gctggactgg ttgctcacac aactggaagc
     9301 catcccgcat atcaaacgtc tgcggattca cagccgtctg ccgattgtga tcccggcacg
     9361 catcacagat gcgctggttg aacgcttttc acattctacg ctgcaaatcc tgctggtgaa
     9421 tcacatcaac catgccaatg agatagatga aacattccgt caggcgatgg ctaagttgcg
     9481 ccgggtaggc gttactttgc tgaaccagag cgttctgtta cgtgatgtga acgataacgc
     9541 acaaacgctg gcaaacctga gtaatgcgtt gttcgatgcc ggcgtgatgc cgtattacct
     9601 gcatgtgctc gataaagtac agggcgcagc gcattttatg gtgagtgatg acgaagcacg
     9661 gcagattatg cgtgagttgc tgacattggt gtcgggatat ctggtgccga aactggcgcg
     9721 agaaattggc ggcgaaccca gcaaaacgcc gctggatctc cagctacgcc agcagttact
     9781 gattaaacag ggaagttaaa cattaaaatt gcctgatgcg ctacgcttat caggcctaca
     9841 tcgtgcattg aaattgaatg gatttgcaat gaactgtagg ccggataagg cgtttacgcc
     9901 gcatccggca agagcaacaa acgcaagtac agcaacctgt cattaaactg agaaatttaa
     9961 tctttgcccg ttgcataacc tttaattatt catatgcatt gctgttgtta ctttttatat
    10021 agctaacacc aataattaac ctttcttttc tgtttatata gagtgagcac ggctatttat
    10081 gttattttaa caactattac taccaagccc atttatctgg cttataaatt aaaattaatt
    10141 atttaattga gaaatggtta gggagaacct acatggccat aagcatcaag ggtgttaata
    10201 ccggtgttat tcgcaagagt aataatttca ttgcactggc gctgaaaatt aaagaacctc
    10261 gcaataaaga atcgttgttc tttatgtctg tcatggaact gcgcgacctg ctgattgcgc
    10321 tggaaagtcg tctgcatcaa aagcataaac tggatgctgc tgcacgcctt cagtatgagc
    10381 aagctcgcga taaagtcatt aagaaaatgg cagaaaacat cccagaaatt ctggttgatg
    10441 agcttaaaaa tgccgatatt aatcgtcggg ttaacacgct ggaacttacc gataatcaag
    10501 gcgaaaatct taccttcgta ttaacgctac atgacggtag caaatgtgag ttagtcgtca
    10561 atgagttgca gattgaaatg ctagcgcgag caatcattca tgcaattaat aatgcggaaa
    10621 tgcgtgaact ggcattacgt attacctcac tgttagattt cctgccgtta tatgacgttg
    10681 actgccagga aaatggcaat ctggaatatg acacctattc ccaaccggaa tggaaacata
    10741 acttgttcga ccattatctg gcggtgcttt accgctttaa agatgaaagt ggcaaagaac
    10801 aatttagcgg cgcagtggtg aaaacgcgtg aagcaacgcc gggtaaagaa gtagaggcga
    10861 tcactcgccg aatgcttgat ttcagcccac gactgaaaaa actcgcgggt gtgccatgtc
    10921 aggtctatgt tcgcaccgta gcggcgaata acgcccaacc gctaacccag gatcaatgct
    10981 tacgcgcgct gcatcactta cgtgttcaat ccaccagcaa aactgcacca caggcgaaat
    11041 aatctcatct ttgctaatgc gaggctggag ccatccagcc tgaaatgact gataacgtga
    11101 tcgttggtca tgccggatgc ggcgtaaacg ccttatccgg cctacaaatt ctcaagcatt
    11161 caatcagttg caatgcctgc tgtaggcctg ataagcgaag cgcatcaggc aataatacgt
    11221 ttgtcaccca gcctctggat cacatcagtt cgggcactta taaacctgac cgataatctg
    11281 gctatccgtc gggacaaaac tggacaacat tccctgcgac gggctgctga tgccataaat
    11341 cacgttaccg cccattgcag ccgcctggtt gcgcagatcg tttgctgcgc cgcgcataga
    11401 accgccctct tctccgtgtt gcccggaaag ccagttgctt tgcttacctg tcgcagtacc
    11461 aatcagctgg cactctgcgc ctggctgctc gtccacaatg cgtacactct gaccggcagc
    11521 actcaattcg ttgctggagc tacaacccgc catcagtagc gcggcaccga caatccctgc
    11581 taagtatttt acgtgcatgt tattccccat aatcaatgag ctggacgcca ctcgcgtcgt
    11641 ccgtgtctga atcttatact agaaagatga ccaaaagaaa aacccccaga catttctgcc
    11701 cgggggtttg tttatttctg cgaggtgcag ggcaattaca tcatgccgcc catgccaccc
    11761 atgccgccca taccgccagc agcgcctaag tcagctgcat cgtttttcgg caggtcggta
    11821 accatgcact cggtggtgat catcaggcca gccacagaag ccgcgtactg cagagcagaa
    11881 cgggttactt tggttggatc caagataccc atgtcgatca tgttgccgta ttcttcggtt
    11941 gctgcgttgt aaccgtagtt gccgtcgccg cctttaacag tgttagcaac aacagacggt
    12001 tcttcgccgc agttcaatac gatctgacgc agcggagctt ccattgcacg cagtgcaact
    12061 ttgataccca cgttctggtc ttcgttctga ccacgcaggt cagccagttt agacgctacg
    12121 cggatcagcg caacaccacc accagcaacc acgccttctt ctaccgcagc acgggtcgcg
    12181 tgcagggcat cttcaacgcg tgcttttttc tctttcattt caacttcggt agcagcacct
    12241 actttgataa ctgcaacgcc gcctgccagt ttcgctacgc gctcctgcag tttttcacgg
    12301 tcgtagtcag aagttgcttc ttcaatctgc tgacggatct gagcaacacg gccctggatt
    12361 gcagcttctt cacccacgcc atcaatgatg gtggtggtgt ctttgttgat cacaacacgt
    12421 ttagcctgac ccaggtcttc cagggttgct ttttccagct ccataccgat ctcttcagag
    12481 attacggtac cgccagtcag ggttgcgata tcctgcagca tagctttacg gcgatcgccg
    12541 aagcccggag ctttaaccgc agcaactttc acgatgccac gcatggtgtt aacaaccaga
    12601 gttgccagcg cttcgccttc aacatcttca gcgatgatca gcagcggttt acctgcttta
    12661 gcaacggctt ccagaaccgg cagcatttcg cggatgttgg agattttctt gtcagccagc
    12721 aggatgaacg ggctttccag ttctactgcg ccagtttccg gcttgttgat gaagtaagga
    12781 gacaggtagc cacggtcgaa ctgcatacct tcaaccacgt ccagttcgtc ctgcagaccg
    12841 gtaccgtctt caacggtgat aacgccttct ttaccgactt tgtccatcgc ttcagcgatc
    12901 agtttaccta cggtttcgtc ggagttagcg gagatggtac caacctgagc aatcgcttta
    12961 gagtcagagc atggtacgga cagcgctttc agttcttcaa ctgcagcggt aacagctttg
    13021 tcgataccac gtttcaggtc catcgggttc atgcccgcag caactgcttt cagaccctca
    13081 gtgatgatag cctgagccag tacggttgca gtggtggtac cgtcgcctgc agcgtcgttc
    13141 gctttagagg caacttcttt caccatctgc gcacccatat tttcgaactt gtcttccagt
    13201 tcgatttcac gagcaacgga aacaccatct ttggtgatgg tcggtgcacc gaaagattta
    13261 tccagaacta cgttacggcc tttcggaccg agggtaactt tcactgcatc tgccagtacg
    13321 tttacgccgc gcagcatttt cacacgagcg tcgttaccga attttacgtc tttagctgcc
    13381 attatcttta ttccttaaat tcgtatgttc agtgtcgtgc gaggattacg cttcaacaat
    13441 tgccagaatg tcgctttcgg acatgatcaa cacttcttca ttgtcgatct tctcagattt
    13501 cacaccgtag ccatcgttga aaataacgat gtcgccaact ttcacatcca gcggcttcac
    13561 ttcgccattt tcaaggatac ggccattgcc gacagccagc acttcgccgc gggtggattt
    13621 agccgctgca gagccggtca gaacgatgcc gccagcagat ttagtttcaa cttctttacg
    13681 cttgacgatc acgcgatcat gcaatggacg aatattcatt gataactctc ctttgagaaa
    13741 gtccgtatct gttatgggtg acgccggagc ttacgtggtt tcccggctgg tgaccagaga
    13801 aatggggatg aggattcgcc ccttcaaggg ggaaaaagaa aaaaaattct gatcagcaca
    13861 aaatcgggtg aaaaccctga ttcacctcac atttcatcgc aatttcttca tcgccgtcat
    13921 aagcgaatct gattgtgcta ccatcgaaaa tctacgcagt tgcccaaaat ttgggcgcaa
    13981 tcggatatca acccatgagt ggactcaaac aagaactggg gctggcccag ggcattggcc
    14041 tgctatcgac gtcattatta ggcaccggcg tgtttgccgt tcctgcgtta gctgcacagg
    14101 tagcaggcaa taacagcctg tgggcgtggc ccgttttgat tatcttagtg ttcccgattg
    14161 cgattgtgtt tgcgattctg ggtcgccact atccctgcgc aggcggtgtc gcgcacttcg
    14221 tcggtatggc gtttggttcg cggcttgagc gagtcaccgg ctggttgttt ttatcggtca
    14281 ttcccgtggg tttgccagcc gcgctacaaa ttgccgccgg gttcggccag gcaatgtttg
    14341 gctggcatag ctggcaactg ttgttggcag aactcggtac gctggcgctg gtgtggtata
    14401 tcggtactcg cagtgccagt tccagtgcta atctacaaac cgttattgcc ggacttatcg
    14461 tcgcgctgat tgtcgctatc tggtgggcgg gcgatatcaa acctgcgaat atccccttcc
    14521 cggcacctgg taatatcgaa cttaccgggt tatttgctgc gttatcagtg atgttctggt
    14581 gttttgtcgg tctggaagca tttgcccatc ttgcctcgga atttaaaaat ccagagcgtg
    14641 attttcctcg cgcattgatg attggtctgc tgctggcagg attagtctac tggggctgta
    14701 cggtagtcgt cttacacttc gacgcctatg gtgaaaaaat ggcggcggca gcatcgcttc
    14761 caaaaattgt agtgcaactg ttcggtgtag gagcgttatg gattgcctgc gtgattggct
    14821 atctggcctg ctttgccagt ctcaacattt atatacagag cttcgcccgc ctggtctggt
    14881 cgcaggcgca acataatcct gaccactacc tggcacgcct ctcttctcgc catatcccga
    14941 ataatgccct caatgcggtg ctcggctgct gtgtggtgag cactttggtg attcatgctt
    15001 tagagatcaa tctggacgct cttattattt atgccaatgg catctttatt atgatttatc
    15061 tgttatgcat gctggcaggc tgtaaattat tgcaaggacg ttatcgacta ctggcggtgg
    15121 ttggcgggct gttatgcgtt ctgttactgg caatggtcgg ctggaaaagt ctctatgcgc
    15181 tgatcgtgct ggcggggtta tggctgttgc tgccaaaacg aaaaacgccg gaaaatggca
    15241 taatcacata atccggcgtt tcgacattaa tcctggcgat cgtctttatg atcaaggcgg
    15301 tcgcgctcat catcctttcg ctggtactca ccatcaaaag tattaccgcc accggtcccg
    15361 gcgctaaaac cgccgcctgg catacgagaa aagcgcaaat gcggcatcaa cttcactgtc
    15421 agatgctttt gcaccggcgg taataaaagc agaagaccga ggaagtcggt gaaaaagccc
    15481 ggtaataaaa gcagcaaacc ggcaatgatc agcgaaacac ttttaatcat ctccgccgct
    15541 gggttttcac ccgccgccat tttttgctgc atcagcacga aattcttaaa gccctggtta
    15601 cgtaccagtg acatcccgat aactgacgtg aatataacca gcacgagggt cagcaatacc
    15661 cccaatacat gggcaacctg aataaagatt gaaatctcaa tatagacata aaggaaaatg
    15721 gcaataaaag gtaaccagcg caaaggtttc tcctgtaata gcagccggta aaacccggct
    15781 acctgaatgg gttgcgaatc gcgttcagct tatattgtgg tcattagcaa aatttcaaga
    15841 tgtttgcgca actatttttg gtagtaatcc caaagcggtg atctatttca caaattaata
    15901 attaaggggt aaaaaccgac acttaaagtg atccagatta cggtagaaat cctcaagcag
    15961 catatgatct cgggtattcg gtcgatgcag gggataatcg tcggtcgaaa aacattcgaa
    16021 accacatata ttctgtgtgt ttaaagcaaa tcattggcag cttgaaaaag aaggttcaca
    16081 tgtcaaacaa cattcgtatc gaagaagatc tgttgggtac cagggaagtt ccagctgatg
    16141 cctactatgg tgttcacact ctgagagcga ttgaaaactt ctatatcagc aacaacaaaa
    16201 tcagtgatat tcctgaattt gttcgcggta tggtaatggt taaaaaagcc gcagctatgg
    16261 caaacaaaga gctgcaaacc attcctaaaa gtgtagcgaa tgccatcatt gccgcatgtg
    16321 atgaagtcct gaacaacgga aaatgcatgg atcagttccc ggtagacgtc taccagggcg
    16381 gcgcaggtac ttccgtaaac atgaacacca acgaagtgct ggccaatatc ggtctggaac
    16441 tgatgggtca ccagaaaggt gaatatcagt acctgaaccc gaacgaccat gttaacaaat
    16501 gtcagtccac taacgacgcc tacccgaccg gtttccgtat cgcagtttac tcttccctga
    16561 ttaagctggt agatgcgatt aaccaactgc gtgaaggctt tgaacgtaaa gctgtcgaat
    16621 tccaggacat cctgaaaatg ggtcgtaccc agctgcagga cgcagtaccg atgaccctcg
    16681 gtcaggaatt ccgcgctttc agcatcctgc tgaaagaaga agtgaaaaat atccaacgta
    16741 ccgctgaact gctgctggaa gttaaccttg gcgcaacagc aatcggtact ggtctgaaca
    16801 cgccgaaaga gtactctccg ctggcagtga aaaaactggc tgaagtcact ggcttcccat
    16861 gcgtaccggc tgaagacctg atcgaagcga cctctgactg cggcgcttat gttatggttc
    16921 acggcgcgct gaaacgcctg gctgtgaaga tgtccaaaat ctgtaacgac ctgcgcttgc
    16981 tctcttctgg cccacgtgcc ggcctgaacg agatcaacct gccggaactg caggcgggct
    17041 cttccatcat gccagctaaa gtaaacccgg ttgttccgga agtggttaac caggtatgct
    17101 tcaaagtcat cggtaacgac accactgtta ccatggcagc agaagcaggt cagctgcagt
    17161 tgaacgttat ggagccggtc attggccagg ccatgttcga atccgttcac attctgacca
    17221 acgcttgcta caacctgctg gaaaaatgca ttaacggcat cactgctaac aaagaagtgt
    17281 gcgaaggtta cgtttacaac tctatcggta tcgttactta cctgaacccg ttcatcggtc
    17341 accacaacgg tgacatcgtg ggtaaaatct gtgccgaaac cggtaagagt gtacgtgaag
    17401 tcgttctgga acgcggtctg ttgactgaag cggaacttga cgatattttc tccgtacaga
    17461 atctgatgca cccggcttac aaagcaaaac gctatactga tgaaagcgaa cagtaatcgt
    17521 acagggtagt acaaataaag aaggcacgtc agatgacgtg ccttttttct tgtgaacagt
    17581 aacttaaaaa caacaatcta atatcaactt gttaaaaaac aaggaaggct aatatgctag
    17641 ttgtagaact catcatagtt ttgctggcga tcttcttggg cgccagattg gggggaatag
    17701 gtattggttt tgcaggcgga ttgggggtgc tggttcttgc cgctattggc gttaaacccg
    17761 gtaacatccc gttcgatgtc atttccatta tcatggcggt tatcgccgct atttctgcca
    17821 tgcaggttgc tggcggtctg gactatctgg ttcatcagac agaaaagctg ctgcgccgta
    17881 acccgaaata catcacgatc ctcgcaccta tcgtgaccta tttcctgaca atctttgctg
    17941 gtactggcaa catctctctg gcgacactgc cagttatcgc tgaagttgcg aaggaacaag
    18001 gcgttaaacc ttgccgtccg ctctctactg cagtggtatc cgcgcagatt gcgatcaccg
    18061 catcgccaat ctcagcggca gtggtttaca tgtcttccgt gatggaaggt catggcatca
    18121 gctacctcca tctgctctcc gtggtcatcc cgtccaccct gctggcggtt ctggtgatgt
    18181 cctttctggt cactatgctg ttcaactcca aactctctga cgatccgatt tatcgcaagc
    18241 gtctggaaga gggcctggtt gaactgcgcg gtgaaaagca gattgaaatc aaatccggtg
    18301 caaaaacgtc cgtctggctg ttcctgctgg gcgtagttgg cgtggttatc tatgcaatca
    18361 tcaacagccc aagcatgggt ctggttgaaa aaccgctgat gaacaccacc aacgcaatcc
    18421 tgatcatcat gctcagcgtt gcaactctga ccaccgttat ctgtaaagtc gataccgaca
    18481 acatcctcaa ctccagcacc ttcaaagcag gtatgagcgc ctgtatttgt atcctgggtg
    18541 ttgcgtggct gggcgatact ttcgtttcca acaacatcga ctggatcaaa gataccgctg
    18601 gtgaagtgat tcagggtcat ccgtggctgc tggccgtcat cttcttcttt gcttctgctc
    18661 tgctgtactc tcaggctgca accgcaaaag cactgatgcc gatggctctg gcactgaacg
    18721 tttcaccgct gaccgctgtt gcttctttcg ctgcggtgtc tggtctgttc attctgccga
    18781 cctacccgac gctggttgct gcggtacaga tggatgacac gggtactacc cgtatcggta
    18841 aattcgtctt caaccatccg ttcttcatcc cgggtactct gggtgttgcc ctggccgttt
    18901 gcttcggctt cgtgctgggt agcttcatgc tgtaatgatc aatcgcgggg cgttcacgcc
    18961 ccgctttctt tcccgccgac taacatcctt cccccgtacg ttgtatagtg acctctctct
    19021 tgcggttcca tctgttcttg cgaggtgttt atgcttgatg aaaaaagttc gaataccgcg
    19081 tctgtcgtgg tgctatgtac ggcaccagat gaagcgacag cccaggattt agctgccaaa
    19141 gtgctggcgg aaaaactggc ggcctgcgcg accttgatcc ccggcgctac ctctctctat
    19201 tactgggaag gtaagctgga gcaagaatac gaagtgcaga tgattttaaa aactaccgta
    19261 tctcaccagc aggcactgct ggaatgcctg aagtctcatc atccatatca aaccccggaa
    19321 cttctggttt tacctgttac acacggagac acagattacc tctcatggct caacgcatct
    19381 ttacgctgat cctgctactt tgcagcactt ccgtttttgc cggattattc gacgcgccgg
    19441 gacgttcaca atttgtcccc gcggatcaag cctttacttt tgattttcag caaaaccaac
    19501 atgaccttaa tctgacctgg cagatcaaag acggttacta cctctaccgt aaacagatcc
    19561 gcattacgcc ggaacacgcg aaaattgccg acgtgcagct gccgcaaggc gtctggcatg
    19621 aagatgagtt ttacggcaaa agcgagattt accgcgatcg gctgacgctt cccgtaacca
    19681 tcaaccaggc gagtgcggga gcgacgttaa ctgtcaccta ccagggctgt gctgatgccg
    19741 gtttctgtta tccgccagaa accaaaaccg ttccgttaag cgaagtggtc gccaacaacg
    19801 cagcgtcaca gcctgtgtct gtttcgcagc aagagcagca caccgcgcaa ttgccctttt
    19861 ccgcgctctg ggcgttgttg atcggtattg gtatcgcctt tacgccatgc gtgctgccaa
    19921 tgtacccact gatttctggc atcgtgctgg gtggtaaaca gcggctctcc actgccagag
    19981 cattgttgct gacctttatt tatgtgcagg ggatggcact gacttacacg gcgctgggtc
    20041 tggtagttgc cgccgcaggg ttacagttcc aggcggcgct acagcaccca tacgtgctca
    20101 ttggcctcgc catcgtcttt accttgctgg cgatgtcaat gtttggcttg tttactctac
    20161 aactcccctc ttcgctgcaa acacgtctca cgttgatgag caatcgccaa cagggcggct
    20221 cacctggcgg tgtgtttgtt atgggggcga ttgccggact tatctgttca ccatgcacca
    20281 ccgcaccgct tagcgcgatt ctgctgtata tcgcccaaag cgggaacatg tggctgggcg
    20341 gcggcacgct ttatctctat gcgttgggca tgggcctgcc gctgatgcta attaccgtct
    20401 ttggtaaccg cttgctgccg aaaagcggcc cgtggatgga acaagtcaaa accgcgtttg
    20461 gttttgtgat cctcgcactg ccggtcttcc tgctggagcg agtgattggt gatgtatggg
    20521 gattacgctt gtggtcggcg ctgggtgtcg cattctttgg ctgggccttt atcaccagcc
    20581 tacaggctaa acgcggctgg atgcgcgtgg tgcaaataat cctgctggca gcggcattgg
    20641 ttagcgtgcg cccacttcag gattgggcat ttggtgcgac gcataccgcg caaactcaga
    20701 cgcatctcaa ctttacacaa atcaaaacgg tagatgagtt aaatcatgcg ctcgttgaag
    20761 ccaaaggcaa accggtgatg ttagatcttt atgccgactg gtgcgtcgcc tgtaaagagt
    20821 ttgagaaata caccttcagc gacccgcagg tgcaaaaagc gttagcagac acggtcttac
    20881 ttcaggccaa catcacggcc aacgacgcac aagatgtggc gctgttaaag catcttaatg
    20941 tccttggcct accgacaatt ctcttttttg acggacaagg ccaggagcat ccacaagcac
    21001 gcgtcacggg ctttatggat gctgaaacct tcagcgcaca tttgcgcgat cgccaaccgt
    21061 gaacgacact tgcagcggga aagacggagg aaaatagcgt gcaacgtgaa gatgtactgg
    21121 gagaagccct gaaattatta gaattacaag ggattgccaa caccacgctg gagatggttg
    21181 ctgaacgtgt ggattatcca ctggacgagc tacgccgctt ctggccagac aaagaggcaa
    21241 tcctctacga tgcgctgcgt tatcttagcc aacagataga tgtctggcgt cgtcagctga
    21301 tgctggacga aacgcaaacc gccgaacaaa agcagctggc acgttatcag gcgttatcgg
    21361 agtgcgttaa aaacaaccgc tatccgggct gtctatttat cgctgcctgt acgttttatc
    21421 ccgatcctgg ccaccctatt catcaactgg ccgatcagca aaaaagcgcg gcctacgatt
    21481 tcacccacga actgttaacc acactggaag ttgacgatcc ggcgatggta gcaaagcaga
    21541 tggaactggt gctggaaggc tgtttaagcc gaatgctggt gaatcgtagc caggcggatg
    21601 tcgacactgc acatcggctg gcggaagata tcctgcgctt cgcccgctgc cgccagggtg
    21661 gtgcattgac ctgacagaaa cacagaaaag aagcgatttg ccgcaatctt aagcagttga
    21721 atcgctttta ctgaaattag gttgacgaga tgtgcagatt acggtttaat gcgccccgtt
    21781 gcccggatag ctcagtcggt agagcagggg attgaaaatc cccgtgtcct tggttcgatt
    21841 ccgagtccgg gcaccaaatt catatcaacg gacctctacg gaggttcgtt tttcgtttca
    21901 gaacgccacg atttaagcgt tctgcctaca aatcaattct accgaactca accagattcc
    21961 ccccacatca accccattgt gtgggtataa ttgcgggcat accccagttc gacagaattt
    22021 gtaccctctt taacgcattc agaaggctga actatggcac tgactgacgc aaaaatccgg
    22081 gctgcaaagc ccactgacaa ggcttataaa ctcactgacg ggactggcat gttcctgctg
    22141 gtacatccta atggctcccg ttactggcgt ctccgttatc gtattctggg taaggagaag
    22201 actctggcac ttggtgtgta tccagaagtt tctctctccg aagctcgtac aaaacgggat
    22261 gaggcccgaa aactgatttc ggagggggtt gacccttgcg aacagaaaag agctaaaaaa
    22321 gtagtccctg atttacagct ctcttttgaa catattgcgc gacgctggca tgccagtaat
    22381 aaacaatggg cacaatcaca cagcgataaa gtactcaaaa gcctcgagac tcacgttttc
    22441 ccctttatcg gcaaccggga tatcacaaca ctcaataccc cggatctgct tatccctgtt
    22501 cgtgctgcag aagctaaaca aatttatgaa atcgccagtc gtctgcagca aagaatatct
    22561 gccgtaatgc gttatgccgt acagtctggc atcatcagat ataatcctgc tctggatatg
    22621 gctggcgcat tgactacggt aaaacgccag catcgccccg ctcttgatct ttctcgcctg
    22681 cctgaacttt tgtcgcgtat tggcagttac aaagggcaaa ctgtcaccca gcttgccgtt
    22741 atgctgaatt tactggtttt tattcgttcc agtgaactca gatacgcccg ctggtctgaa
    22801 attgatattg acaatgccat gtggactatt ccagccgaac gcgaacccct gccaggcgta
    22861 aaattctcac accggggctc caagatgcga acaccacatc ttgtgccact cagcaaacag
    22921 gctgtagcca tactgacaga actacagaca tgggctggtg aaaatggtct gatctttacg
    22981 ggagcacatg acccgcgtaa accaatcagt gaaaatgtaa tgaacgcatc tcacacaatt
    23041 aaacacggag cagtatgatg gttaaaaggg tgaaactggc aggtgtggag cctcgcagat
    23101 ttccggcatc atagtgccca atacgggatg cataaagctc cctcagactg gagactccac
    23161 aaatgaaata tacaccggtt ggcgttgata tcgcaaaaca tgtcattcag attcacttca
    23221 tcaatgagca cacag
//
LOCUS       DAJTMC010000054        22931 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.054, whole genome shotgun sequence.
ACCESSION   DAJTMC010000054 DAJTMC010000000
VERSION     DAJTMC010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22931
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.054"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(<1..745)
                     /gene="fsr"
                     /locus_tag="OO130_001863"
     CDS             complement(<1..745)
                     /gene="fsr"
                     /locus_tag="OO130_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HCR5365302.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSVGAVLLAAALVGTSSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLTAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISS"
     gene            963..2615
                     /gene="ushA"
                     /locus_tag="OO130_001864"
     CDS             963..2615
                     /gene="ushA"
                     /locus_tag="OO130_001864"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HCR5365303.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDVEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQAPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVTKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(2652..3131)
                     /gene="ybaK"
                     /locus_tag="OO130_001865"
     CDS             complement(2652..3131)
                     /gene="ybaK"
                     /locus_tag="OO130_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HCR5365304.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMAAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(3335..4129)
                     /locus_tag="OO130_001866"
     CDS             complement(3335..4129)
                     /locus_tag="OO130_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HCR5365305.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMTPLPTRLLKKLKNADALIVEADVSTSDTPFANLPTCEALEERINEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQITMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            4267..4608
                     /locus_tag="OO130_001867"
     CDS             4267..4608
                     /locus_tag="OO130_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCR5365306.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSTL
                     INNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(4823..7327)
                     /gene="copA"
                     /locus_tag="OO130_001868"
     CDS             complement(4823..7327)
                     /gene="copA"
                     /locus_tag="OO130_001868"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HCR5365307.1"
                     /translation="MSQTIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPLW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARVVTAEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGESVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALI
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNPALLNDQQVDTKAIEADISA
                     QASQGATPVLLAIDGKVVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            7589..8521
                     /gene="glsA"
                     /locus_tag="OO130_001869"
     CDS             7589..8521
                     /gene="glsA"
                     /locus_tag="OO130_001869"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HCR5365308.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAVVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENIEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGLN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            8524..9816
                     /locus_tag="OO130_001870"
     CDS             8524..9816
                     /locus_tag="OO130_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5365309.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVAHAFGAYAVQFLHEGSQEEHLILLYALGSIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGSLLLSLIVAMVMKRNKTV"
     gene            9941..10348
                     /gene="cueR"
                     /locus_tag="OO130_001871"
     CDS             9941..10348
                     /gene="cueR"
                     /locus_tag="OO130_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HCR5365310.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(10349..10807)
                     /locus_tag="OO130_001872"
     CDS             complement(10349..10807)
                     /locus_tag="OO130_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HCR5365311.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(10804..11721)
                     /locus_tag="OO130_001873"
     CDS             complement(10804..11721)
                     /locus_tag="OO130_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HCR5365312.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            11867..12544
                     /gene="fetA"
                     /locus_tag="OO130_001874"
     CDS             11867..12544
                     /gene="fetA"
                     /locus_tag="OO130_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HCR5365313.1"
                     /translation="MQENSPLLQLQNVGYLAGDAKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNRQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            12531..13310
                     /gene="fetB"
                     /locus_tag="OO130_001875"
     CDS             12531..13310
                     /gene="fetB"
                     /locus_tag="OO130_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HCR5365314.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDIFWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(13373..14227)
                     /gene="cnoX"
                     /locus_tag="OO130_001876"
     CDS             complement(13373..14227)
                     /gene="cnoX"
                     /locus_tag="OO130_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HCR5365315.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(14288..15097)
                     /gene="ybbO"
                     /locus_tag="OO130_001877"
     CDS             complement(14288..15097)
                     /gene="ybbO"
                     /locus_tag="OO130_001877"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HCR5365316.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNNMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            complement(15087..15710)
                     /gene="tesA"
                     /locus_tag="OO130_001878"
     CDS             complement(15087..15710)
                     /gene="tesA"
                     /locus_tag="OO130_001878"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HCR5365317.1"
                     /translation="MNFNNVFRWHLPFLFLVLLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            15681..16367
                     /locus_tag="OO130_001879"
     CDS             15681..16367
                     /locus_tag="OO130_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5365318.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            16364..18775
                     /locus_tag="OO130_001880"
                     /pseudo
     CDS             16364..18775
                     /locus_tag="OO130_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            19204..22931
                     /locus_tag="OO130_001881"
                     /pseudo
     CDS             19204..22931
                     /locus_tag="OO130_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
BASE COUNT         5291 a         6004 c         6481 g         5155 t
ORIGIN      
        1 agctgctgat gctcgccata tagaaatatt tcgagaaaat gaggattaac agaatgctga
       61 ctgccagtac cactttgttg cgcggcagtg gattgattat cgtcgctttg ggttttcctt
      121 tattcattcg gtgctgtgcc gagtaccaac ggctaatttg cgccaacacc acgatcgcca
      181 gcagtgccgc aagcacaaac caggcaacgt tgcctttgcc ataaggcgcg ataatcaccg
      241 ccgtcagcaa gggtcccagg gaactgccaa agttgccgcc gacctgaaag atagattgcg
      301 ccaggccatg ccgcccgccg gaagccatac gggctacgcg agaagattcc ggatgaaaga
      361 ccgatgaact ggtaccgacc agcgccgccg ccagcagaac tgcgccaaca ctgcccgcca
      421 gcgcaagcag caccagacca cttaaggtaa agcacatgcc aattggcaac gaccacggca
      481 tcggatattt atcggtccag tagccgacca ctggttgcag tagcgaagag gcgagctgga
      541 aggtgagggt tatcatgcca atctgcataa atgtcagaga aaattctgac tgaagcagag
      601 gataaatcgc cagaatcagc gattggatca tgtcgttcag cagatgtgag aggctgatag
      661 cacctaaaat accaaacgat gttcgggcct tggtcgttga cgcagccgcg cccgccacag
      721 gctgggtttg ttcactcatt gccataggaa agtcactttt tcagggttgc gatgtaaaga
      781 atgatcttat ttgtgattat tacccgacta acatacctgt aagcgtcgcc tgaaggaagt
      841 ctcaacgccg aatacagaat ttctaatctg gatgcagatt tatcttcacc ggacgcagac
      901 ttgtctatta tgttgcgtca tactattttt caacacgttg aaatcaggtc agggagagaa
      961 gtatgaaatt attgcagcgg ggcgtggcgt tagcgctgtt aaccacattt acactggcga
     1021 gtgaaactgc tctggcgtat gagcaggata aaacctacaa aattacagtt ctgcatacca
     1081 atgatcatca tgggcatttt tggcgcaatg aatatggcga atatggtctg gcggcgcaaa
     1141 aaacgctggt ggatggtatc cgcaaagagg ttgcggctga aggcggtagc gtgctgctac
     1201 tttccggtgg cgacattaac actggcgtgc ccgagtctga cttacaggat gccgaacctg
     1261 attttcgcgg tatgaatctg gtgggctatg acgcgatggc gatcggtaat catgaatttg
     1321 ataatccgct caccgtatta cgccagcagg aaaagtgggc caagttcccg ttgctttccg
     1381 cgaatatcta ccagaaaagt accggcgagc gcctgtttaa accatgggcg ctgtttaagc
     1441 gtcaggatct gaaaattgcc gtcattgggc tgacaaccga tgacacagca aaaattggta
     1501 acccggaata cttcactgat gtcgaatttc gtaagcccgc cgatgaagcg aagctggtga
     1561 ttcaggagct gcaacagaca gaaaagccag acattattat cgcggcgacc catatggggc
     1621 attacgataa tggtgagcac ggctctaacg caccgggtga tgtggagatg gcacgcgcgc
     1681 tgcctgccgg atcgctggcg atgatcgtcg gtggtcactc gcaagctccg gtctgcatgg
     1741 cggcagaaaa caaaaaacag gtcgattacg tgccgggtac gccatgcaaa ccagatcaac
     1801 aaaacggcat ctggattgtg caggcgcatg agtggggcaa atacgtggga cgggctgatt
     1861 ttgagtttcg taatggcgaa atgaaaatgg ttaactacca gctgattccg gtgaacctga
     1921 agaagaaagt gacctgggaa gacgggaaaa gcgagcgcgt gctttacact cctgaaatcg
     1981 ctgaaaacca gcaaatgatc tcgctgttat cgccgttcca gaacaaaggc aaagcgcagt
     2041 tggaagtgaa aataggcgaa accaatggtc gtctggaagg cgatcgtgac aaagtgcgtt
     2101 ttgtacagac caatatgggg cggttgattc tggcagccca aatggatcgc actggtgccg
     2161 actttgcggt gatgagtgga ggaggaattc gtgattctat cgaagcaggc gatatcagct
     2221 ataaaaacgt actgaaagtg cagccattcg gcaatgtggt ggtgtatgcc gacatgaccg
     2281 gtaaagaggt gattgattac ctgaccgccg tcgcacaaat gaagccagat tcaggtgcct
     2341 acccgcaatt tgccaacgtt agctttgtga cgaaagacgg caaactcaac gacctgaaaa
     2401 tcaaaggcga accggtcgat ccggcgaaaa cttaccgtat ggcgacatta aacttcaatg
     2461 ccaccggcgg tgatggctat ccgcgccttg ataacaaacc gggctatgtg aataccggct
     2521 ttattgatgc cgaagtgctg aaagcgtata tccagaaaag ctcgccgctg gatgtgagtg
     2581 tttatgaacc gaaaggtgag gtgagctggc agtaatccga aagtgccgga tatttgcatc
     2641 cggcacaatg cttaatcgcg gcgggcgata tcagcaaatt tggcatcgag gatcttcgcc
     2701 agatcgcctg ccgccagttc gatatccagt ccgcgcttgc cgccagaaac ataaatagtg
     2761 gcaaattctt gtgcgggggc gtcgataatc gtcggcagac gttttttctg ccccagtggg
     2821 ctaatccccc caaccaggta tcccgtcgaa cgctgcgcgg ccatcggatc ggccatctca
     2881 actttcttgg cacccagcgc ttttgctact tttttaagat ccagttgacc tgcgaccggc
     2941 gtaacggcca ctgccagatg tttcatatca ccgttcactg ccaccagtag cgttttgtag
     3001 acttgatccg gattcaaacc taattttttg acgacttcat cgccaaaatt ggtttcagcc
     3061 ggatcgtgct cgtaggtatg gatttgaaac gaaatcttgt ttttttcgag taatttaact
     3121 gcgggtgtca tggtaatttt cctgccttaa acaaaaaaac gaagcaagca tacgcctgat
     3181 ggcggtccaa aaaaaagagt catcttgcct aagagtattg gcaggatggt gagattgagc
     3241 gacaatcgag ttacaccgtc gcttaaagtg acggcataat aataaaaaaa tgaaattcct
     3301 ctttgacggg ccaatagcga tattggccat ttttttagcg caacatttgc ggcaaattcc
     3361 cttctccata caggtgtagt gcaccgaccg cgaccacata tcgccccggc ggcatggcgc
     3421 gtaatttatc ccgccaggcg agatttcgct gatgcatcag cacatcgtac agcgactgac
     3481 tgaacgtatt gggcagcgtt atatcattat tttgcggagg tgcattcagc caccagctca
     3541 tcatttgttg cagcaaccgt gcgttggtat gccagtgggt cagcgtatcg tccagcagcg
     3601 ccagtccttt gtcagggagc tggagcaaca tggtaatctg gttttcagcc ccttccagtt
     3661 caatcacggg tttatgttgt tgcttcgccg cctgcaataa ctggtaatcg ataccgtatt
     3721 ctgcccgcag ccccagtttt tgtgcctgcg tcgcctgaag aaccatcgcg atttgccaca
     3781 gcggttgagt agaaaagagc gacggagaaa tgcccatctc ctggctaatg tgctgcagat
     3841 tttggagttg ctcctcgtta atgcgctctt ccagcgcctc gcaagtaggc aaattagcaa
     3901 aaggcgtatc gctggtggaa acatccgcct cgacgatcag cgcatcggcg tttttgagct
     3961 ttttgagcaa acgggtgggc aggggagtca tatcgtggct acccatatga atactgccaa
     4021 tcagatgaaa atggcgattg ccgggaaggg tgatatcgat ggcaggccag gtgtaatgat
     4081 taccgcgcag cgcggcccaa agcgttttta cccggtacaa cagatccata cgacctccct
     4141 ttgtgaaata tcatgctagc gcgcggtgag ggatggcgca aatggagttg gggacttgtt
     4201 taatgtgttt gtatgattca gtatgttctt gcatcgctat tcacagggaa gcaacagtta
     4261 aaaaccatga aacaggcaac aagaaaaccg acgacacccg gagatattct tctctatgaa
     4321 tatctggaac cgctcgattt gaaaatcaat gagttagcag agttgctgca tgttcatcgt
     4381 aatagcgtca gtacactgat caataataat cgtaaactca ccactgagat ggcattccgt
     4441 ctggcgaaag tttttgatac cacagtcgat ttttggctaa acctccaggc ggcagttgat
     4501 ctttgggaag ttgaaaacaa catgcgcacc caggaagaat tgggacggat tgaaacagtg
     4561 gctgaatatt tggcacgccg tgaagagcgt gcaaaaaagg tcgcgtaagg ccaaaacatc
     4621 gcttcatctc gtaggccgga taaggcgttc acgccgcatc cggcatccga gcaccatagc
     4681 ctgatgcgac gcttacccgt cttatcaggc ctacaaacct gtgcctgaac cgtaggccgg
     4741 ataaggcgtt tacgccgcat ccggcagtca tgcgtcgatg ccaaatgcgc cgccctaaag
     4801 cagcgcatcc gcaatgacgt acttattcct tcggtttaaa ccgcagcaac cggttggcgt
     4861 tactcactac ggtaatcgac gagagcgcca ttgccgctcc ggcaactacc gggttaagca
     4921 gtgttccagt aaacggccac aaaataccag cggcgaccgg aataccaatg ctgttgtaga
     4981 taaacgcacc gagcaggttc tgcttcatat tgcgcagcgt tgcgcgggaa atcgcgagcg
     5041 catctgcaac acccatcagg ctatggcgca tcagggtaat cgccgcggtt tcaatggcaa
     5101 catcactgcc gccacccatc gcaataccga catccgcctg agccagcgct ggcgcgtcgt
     5161 taatgccgtc gcccaccatt gccacctgac gtccttcact ttgcagacgt ttgatcgctt
     5221 cggctttacc gtctggcagc accccggcga ttacctcatc aatccctgct tctttggcga
     5281 tcgcattggc ggtggttggg ttatccccgg tcaacatcac cagacgatat cccgctttat
     5341 gcaggcgttg cagcgccgcc acgctatcac tacgcaacgg atcgcgtaca gccagcaggg
     5401 ctactacttt cccgtcaatc gccagcagca caggcgttgc tccttgcgat gcctgagctg
     5461 aaatatcggc ttcaatcgct ttagtatcga cttgctggtc atttaacagc gccggattac
     5521 ccagcaataa cgcatgacct tcagcttcac cgcttacgcc cagcccgcgc aatgtgcgga
     5581 aaccgttgac ctgcggcagc tgcatatcgc ctgctttatc gaggatcgct cgtgccagcg
     5641 gatggctgga accttgctcc agcgccgccg ccagacgcaa tgcttgcgct tcatcaacat
     5701 cagcaaatgt ttttactgcg acaacctgcg gcttcccttc ggtcagcgtc ccggttttat
     5761 cgaacacgac ggtatcgagt gtactggcgc gttgcagcgc gtcggcgtcc cgcaccagca
     5821 cgccaaactc agccgcccgc ccgacgccgg aaataatcga cattggcgtc gccagcccca
     5881 gcgcacacgg acaggcaata atcagcaccg tggtggctat caccagggta tagacaatct
     5941 gcggtgccgg accaaagaaa taccagattg ctgcactgat cagcgcaatc accactacta
     6001 ccggcacaaa tacggctgag attttatccg ccagctgacc gatttctggc ttgctgctct
     6061 gggcctggcg caccatgcga ataatccttg acagcgtggt atggctgcca accgcactgg
     6121 cgcgaaatag cacgctaccg tcctgtacca ctgtcccggc atggacgctt tcgccttcgc
     6181 ctttttgctg cgggattggt tcgcccgtca gcatcgcttc atccagccat gcttcgccct
     6241 gggtaatctc gccatctacc ggcacgcgat cgccggtcgt caggcgcagt aacatccctg
     6301 gctgaacttc cgccagcggc acgctttttt caccctctgc ggtgaccact cgcgccgtcg
     6361 gtggggttaa atcgagtaat ttctccagcg ccttagaaga acgctgacgc gcgcgcgctt
     6421 ccagcatatg gccgagatta atcagaccga taatcatcgc actggcttca tagtaaagat
     6481 gccgcgcttc catcgggaac cacagcggcc acaggttgac gctcatcgaa tagagccacg
     6541 ccacacccgt acccagcgcc accagcgtat ccatcgtcgc cgcaccgttc agcaggcttt
     6601 tccacgcgct gcggtaaaaa tgcccgccgg cgaaaaccat taccgccagg gttatcaggc
     6661 cgataactaa ccacaggctg cggttgtcag cggtgaccat catgttatcg ccgatcatcc
     6721 cccagaccat taccggaata cccaccgcca gtgcgacaat cgcctgccag cggaagcgct
     6781 tcatcgtagc gacggcggtt tcctgctggc gctcgcggcg tttcgcgtca tcttcaatcg
     6841 cttccgcgcc gtagcccgct ttttccaccg cctgcactaa atcttgtggg gaggcactgc
     6901 ccatcaccag cgcagtacgc tccgccaggt ttacccgtgc ctgagtgacg cccggtacgc
     6961 tttgcagcgc attttgtacg cgggtgacac agctggcgca gctcatgccg ctcagcagca
     7021 actgctggct gtcatcgtca tcggcggtcg ctgccggaag cgcctcagaa accgctgtca
     7081 gtgcttccga cgggattgat gactccgcca gcggtttagc ctttgggtgg cttacagatg
     7141 cgtcataacc cgcttgtttg atggtttcga tcagctgttc tgcactggca gtcccggtaa
     7201 cgtgcgcttc agtgatagac acatccgctt gttccacatc cgggcgctgt tcgagacttt
     7261 ctttcacgcg tttaacgcag tgaccgcagg acaggccgtc cagggtcagg tcgatagttt
     7321 gtgacataaa acactccttt aagacagttt tgactggctg tgataaaggt taaaccttcc
     7381 agcaagggga aggtcaagaa attaataaac caggcgggta aaagtccgta aagattaaaa
     7441 aatcggctcg atttgcatca ggattagaca tttatttctt tgttttcctg tagttaagtt
     7501 gctggtgcta agttaaatca ggatgcctga aaatcggcac cggggtgagg aattacctcc
     7561 cgcatctata aaaaggagtt aacaaaagat gttagatgca aacaaattac agcaggcagt
     7621 ggatcaggct tacacccaat ttcactcact taacggcgga caaaatgccg attacattcc
     7681 ctttctggcg aatgtaccag gtcaactggc ggtagtggct atcgtgacct gcgatggcaa
     7741 cgtctatagt gcgggtgaca gtgattaccg ctttgcactg gaatccatct cgaaagtctg
     7801 tacgttagcc cttgcgttag aagatgtcgg cccgcaggcg gtacaggaca aaattggcgc
     7861 tgacccgacc ggattgccct ttaactcagt tatcgcctta gagttgcatg gcggcaaacc
     7921 gctttcgcca ctggtaaatg ctggcgctat tgccaccacc agcctgatta acgctgaaaa
     7981 tattgaacaa cgctggcagc gaattttaca tatccaacag caactggctg gtgagcaggt
     8041 agcgctctct gacgaagtca accagtcgga acaaacaacc aacttccata accgggccat
     8101 agcctggctg ctgtactccg ccggatatct ctattgtgat gcaatggaag cctgtgacgt
     8161 gtacacccgt cagtgctcca cgctcatcaa tactgttgaa ctggcaacgc ttggcgcgac
     8221 gctggcggcg ggtggtctga atccgctgac gcataaacgc gttcttcagg ccgacaacgt
     8281 gccgtacatt ctggccgaaa tgatgatgga agggctgtat ggtcgctccg gtgactgggc
     8341 gtatcgcgtt ggtttaccgg gcaaaagcgg tgtaggtggc ggtattctgg cggtcgtccc
     8401 tggcgtgatg ggaattgccg cgttctcacc accgctggac gaagaaggta acagtgttcg
     8461 cggtcaaaaa atggtggcat cggtcgctaa gcaactcggc tataacgtgt ttaagggctg
     8521 atcatgatga acacggaagg taataacggt aacaaacccc tcggtctatg gaatgtcgtt
     8581 tccatcggta ttggggcaat ggtgggggcg gggatcttcg cgctgctggg gcaggctgcg
     8641 ttgctaatgg aagcctcgac ctgggtcgcc tttgcttttg gcggtattgt ggcgatgttt
     8701 tccggttatg cctatgcgcg tctgggggcg agctatccca gcaatggcgg cattatcgac
     8761 ttctttcgtc gcggattagg caacggcgtc ttttcgctgg cgctctcgtt actgtacctg
     8821 ttgacgctgg cggtgagcat cgccatggtc gcccatgctt ttggcgctta tgccgtgcag
     8881 tttttgcatg aaggcagcca ggaggagcac cttattttgc tctacgcgtt ggggagcatt
     8941 gcggtgatga cgcttttcaa ctccttaagc aaccatgcgg tagggcggct ggaagtgatc
     9001 ctcgtcggca ttaaaatgat gatcctgtta ttgctgatta ttgccggtgt ctggtcgctg
     9061 caaccagcgc atatttccgt ctctgcgccc cccagctccg gtgcgttctt ctcctgtatt
     9121 gggataactt tccttgccta tgcgggcttt ggcatgatgg cgaacgcggc ggataaagtg
     9181 aaagatccgc aggtcattat gccacgggcg tttctggtgg cgattggcgt taccacgttg
     9241 ctttatatct cgctggcact ggttttgctt agcgatgtat cggcattaga gttagaaaaa
     9301 tatgccgata ccgccgtagc gcaggctgct tctccgctgc tcggacatgt gggttatgtg
     9361 atcgtcgtca tcggcgcttt actggcgacg gcttcagcca ttaacgcgaa cctgttcgcc
     9421 gtgtttaaca tcatggacaa catgggcagc gaacgcgaac tgccgaagct aatgaataaa
     9481 tccctgtggc ggcagagtac ctggggcaac atcattgtcg tggtgttgat tatgctgatg
     9541 acggcggcac tgaatttagg ctcactcgcc agcgttgcca gcgccacctt tttgatttgc
     9601 tacctggcgg tgtttgtggt ggcgatccgc ctgcgtcatg atattcacgc ctcgttgccg
     9661 attcttatcg ttggtacgtt ggtgatgttg ttggtgatcg ttggctttat ctacagtctg
     9721 tggtcccagg gtagccgtgc gttgatatgg attattggct cactcttact cagccttatt
     9781 gtggcaatgg tcatgaagcg caataaaacc gtataacatc tctctgtgcg cagtacttcc
     9841 tgtattattg tggtggcggt cgatattcgc actggcaaaa aaacgtgctt gaatatctgt
     9901 tgaaaccctt taacaaagca caggaggcgt tgcgcgaacg atgaacatca gcgatgtagc
     9961 aaaaattacc ggcctgacta gcaaagccat tcgcttctat gaagagaagg ggctggtgac
    10021 gccgccgatg cgcagcgaaa acggttatcg cacctacacg cagcagcatc tcaacgaact
    10081 gaccttactg cgccaggcac ggcaggtggg ctttaacctg gaagagagcg gcgagctggt
    10141 gaatctgttt aacgacccgc agcggcacag cgccgacgtc aaacggcgca cgctggagaa
    10201 ggtggcggag atcgaacgac acattgagga gctgcaatcc atgcgcgacc agctgctggc
    10261 actggcgaat gcctgccctg gcgatgacag cgccgactgc ccgattatcg aaaatctctc
    10321 cggctgctgt catcatcggg cagggtgatt aagacgagac ggctcggata tgtagggtta
    10381 tcccttctat cgcaatgact tcaacatgcg tacctgcacc gagatcctcg ctggcgctga
    10441 caggccatga actgtcaccg acgcgcatat gaccgcgccc gttgaccagc ggagattcca
    10501 gcacaaaacg tcggccaatc agctgctgcc cgcgctggtt taaatgacta tcgctgtgct
    10561 tttgttcgcg cacccgccgc gacaaccatt tccaccacag ccaggcggcg agcagcgtca
    10621 ggatggcaaa cattacccct tgccactccc aacccagcgg caccagccag accaccaggc
    10681 cggtaatcac cgctgccacg ccgctccaca acaaataacc atttccgccc agcatctcgg
    10741 ctgccagcag caaaccgccg agactgagcc agaaaatatg tggatgaacg accattaact
    10801 ccatcatggc tgagtccgtt tgttggcgct gtctttcacc agttcggcaa tcccggcaat
    10861 cgaccccatc aggctgctgg cctctaatgg catcatcact actttgctgt tactggagga
    10921 accgatctgc tgtaacgctt cggtgtattt ctgcgctacg aagtagttca ccgcctgaat
    10981 atcaccggag gcgatagctt cagacaccat tttggtggcg cgggcttctg cttcggcgga
    11041 acgttcacgc gcttcagcct gtaaaaacgc cgactgacgt tcgccttccg ctttcaggat
    11101 ttgcgactgt ttttcacctt cggctttgag gatttccgcc tgacggatcc cttccgcttc
    11161 aagaatgtaa gcgcgtttgg tacgttccgc tttcatctgc gcgttcattg aagagataag
    11221 ctctgccggt gggcgcacgt cgcgaatttc aatacgggtg actttaatcc cccacgggtt
    11281 ggtggcttca tcgacaatac gcagcaggcg tgagttgatg ctgtcgcgct gagagagcat
    11341 ttcgtcaagt tccattgaac ccaacacggt acggatgtta gtcatggtca ggttgatgat
    11401 cgccagctcc agattgctga cttcataggc agcgcgtggc gcgtcaatca cctgaataaa
    11461 acagactgcg tcgatggtaa cgttggcgtt atctttcgag ataacttcct gggaagggat
    11521 atcgagcact tgctccatca tattgatctt gcgaccaatg cgatccataa acggcaccac
    11581 cagactgagc cccggctgta acgttttggt atagcgacca aaacgttcta ccgtccattg
    11641 atagccctgc ggcacgattt tgacgcccgc gccgacaatg accagcgcga caaaaatgag
    11701 aatcgggata aagataagca tcggaaaaac ctcctgttgt accgtccata atcagcaaaa
    11761 ttgctgcttg attaaacaaa ttatacctga ttactgaaag agagttcccc cttattcctg
    11821 cgaaggataa actgttttta gtaaaaatca gaaaaaggga acagcgatgc aggaaaatag
    11881 tcctttgctt cagctacaaa acgtaggata tctggcgggt gatgcgaaga ttcttaataa
    11941 catcaatttt tcgctgcgtg ctggcgaatt taagttaatt accggtcctt ctggttgtgg
    12001 caaaagtacg ctgctaaaaa tagttgcttc attgatcagc ccaaccagcg gaacgttact
    12061 gtttgaaggt gaggatgtca gcacactaaa gccagaaatc taccgccaac aagtctctta
    12121 ctgcgcccag acaccgacgc tgtttggcga tacggtatac gataatctga tctttccttg
    12181 gcagatccgt aaccggcagc ctgacccagc catttttctc gattttctcg aacgcttcgc
    12241 cttgccggac agcattttga cgaaaaatat cgccgagcta tctggtggtg aaaaacaacg
    12301 catctcactg attcgtaacc tgcaatttat gccgaaggtt ttattgctgg atgaaatcac
    12361 cagtgcgctg gatgaaagta ataaacataa cgttaatgag atgatccatc gttatgtgcg
    12421 cgagcaaaat attgccgtgc tgtgggtgac acacgataaa gacgaaatta atcatgcgga
    12481 taaagtgatt acactgcaac cgcatgccgg agaaatgcag gaagcacgct atgaactcgc
    12541 ataatattac taacgaatca ttggcactgg cattaatgct ggtggtggtg gcaatcttaa
    12601 ttagccataa agaaaaactg gcgctggaga aagatatttt ctggagcgtc gggcgcgcga
    12661 taattcagct gattattgtc ggctatgtgc tgaagtatat tttcagcgtg gatgatgcca
    12721 gcctgacatt attgatggtg ttatttatct gctttaatgc ggcgtggaac gcgcaaaaac
    12781 gcagtaaata tattgctaaa gcttttatct catcgtttat tgccattacg gttggggcgg
    12841 gaattaccct ggcggtgctg attctctcag ggtcgattga atttatcccg atgcaggtga
    12901 tccctatcgc cgggatgatt gccggtaacg ccatggtagc ggtggggttg tgttacaaca
    12961 atttagggca acgggtcatt agcgaacagc aacagattca ggagaaactg agtcttggcg
    13021 cgacgccgaa gcaggcttca gcgatattga ttcgcgacag tattcgcgcg gctttaattc
    13081 cgacggtcga ttcagcaaaa acggttggct tagtgagttt accaggaatg atgtccgggc
    13141 tgatatttgc cgggattgat ccggtgaagg cgattaaata tcagattatg gtgaccttta
    13201 tgctgctctc aaccgccagc ttgtcgacca ttattgcctg ctatttaacc tatcgtaagt
    13261 tttataattc gcgccaccag ttggtggtga cgcaattgaa gaagaaatga tgcgatgccg
    13321 gatgcgcagc atcgcatccg gcgttgtggt tcatgtgccg aacaacggaa tatcaataca
    13381 acaatgcata cagctggcgg cgatacttcg acgccagtgc atcacccgta cccagcgcag
    13441 cgaggatctc ctggaacgtt ttacgcgtct gaccgtctgc ggcggtgaga tctttacgca
    13501 gatgcccgaa cagcaactcc agcgcctctt cattgcgccc aacctgatgc agttgcagcg
    13561 ccagttgcgt cgccagtgcg gcatcttctg gattctccgc cacctgctgt tgcaactgtt
    13621 gaatttccgg cgtatcagcc gcctgcttca gcagttcgat ttgcgccacc agcccctggt
    13681 agcgggtgtc ctgatcctgc aacggaatgg ttttcagcac cgcttccgca tcttcagaac
    13741 ggttcagcgc aatcagcgtt tctgccagca gcaggccgat ctccccgttc tgattcgaca
    13801 actgccaggc gtctttcagc aatggcaggg catcggtgta attgccttcc tgcatcagtt
    13861 gcatcgcctg ctgcgctttc agctcttctt cgcgcggcag cactttatcc agcagggcgc
    13921 ggatcgcctc ttccggttgc ggcccctgga agccatctac cggttgcccg ttctggaaca
    13981 gatacacggt cggaatcgcg cgcagaccaa actgggcggc aatcatctgc tccgcgtcgc
    14041 agtccagctt cgccagaata aattgcccgt tgtactgcgc cgcgaggctt tccaggatcg
    14101 gggttaactg caaacagtgc tggctacgtt cagaccaaaa atagaacagc accggagtgg
    14161 tcatcgactg ttcaagaacc tgttgcaggt tagattcgtt aatgttgaca atattttcta
    14221 cggacatgga gtcgctctct gttgtcgata ttttctttga catgggggct taagcgcgcg
    14281 cttcaactca cccctgcaat attttgtcca tcacgcgccc aggcagcagg cgcttgagca
    14341 ccattactgc ccaggtcacc agcgttaccg gatagcgcat cttcggcttc tcgctaataa
    14401 aagcatggcg cactttgtcg accaccgctt ccggtcccaa cgtaaagcgg gcggcgatgc
    14461 cgggattttc gactggttta tcactttgcg tctggttgac gttgtcggtg aagcgagtac
    14521 gaatgggacc gggttcgatc aggctgactt taattccgct gtggcgtagc tccatgcgca
    14581 gcgcgtctga ccacgcctcc agcgcatatt tactggccgc gtaagcgcca cgacccggcg
    14641 tggagattaa tcccatcacc gatgatgtca tcacaatacg cccttcaccg tgcggtaaca
    14701 tcgcgggtaa caggcgcatg gtgagctggt gtgcgccgaa aaagttggcg gaaaactgct
    14761 gttccatctg cgcacggctg atggtggaaa gggggccata catgccgaat ccggcattgt
    14821 taaagatccc atacagacaa ttatcggtca gggcgatcac ctcgtctgct gcgcgatcaa
    14881 cactttctgg tgaatccaga tcgatcaaca cgccggtaaa tcccatgttg ttcatacgct
    14941 caacatcatc cggttttcgg caacctgcca gcacatgaaa accctggcgt tttaattcga
    15001 gcgcgctttc caggccaatt ccactggaac atccggtaat taagaccgat ttttgcataa
    15061 ctttacctgt caggatctcc gttgctttat gagtcatgat ttactaaagg ctgcaactgc
    15121 ttcgccatcc agtcggcaat aaacggctgg gcgtcgcggt tgggatgaat accgtcatcc
    15181 tgcatccatt gtggcttgag gtagacctct tccataaaaa agggcagcag cggaacatca
    15241 aactctttgg cgagtttggg gtaaatggcg ctaaaggctt cattataacg gcgaccatag
    15301 tttgcaggca gacgtatttg cattaacaat ggttcagcgt tggcggcttt gacatcctgc
    15361 aaaatctggc gcagcgtttg ctcggtttgc tgtggctgaa aaccacgcaa accgtcattg
    15421 ccgcccagtt caaccagcac ccagcgcggc tgatgctgtt tcagcagagc cggaaggcgc
    15481 gccagtcctt gttgcgaggt gtcgccgctg atgctggcat taactaccga cgttttactc
    15541 tgccacttat cattcaacaa ggcaggccag gccgcgctgg cagacattcg atacccggcg
    15601 ctcaggctat cacccagaat caataacgtg tccgctgcgg cggcacggaa ggttaacagg
    15661 accaggaaca ggaagggcaa atgccagcgg aaaacattgt tgaagttcat catcttaaga
    15721 agtccgtcgg tcagggggag catgaactct ccatcctcac cggagttgag ctggttgtca
    15781 aacgtggcga gaccatcgca ctggtgggcg agtcgggatc gggtaagtca accttgctgg
    15841 cgatcctcgc cgggcttgat gacggcagca gtggcgaagt gagtctggtg ggacaaccgc
    15901 tacataatat ggacgaagaa gcgcgggcaa agttgcgcgc gaagcacgtc ggctttgttt
    15961 ttcagtcatt tatgttaatt cctaccctta acgcgctgga aaacgtcgag cttccggctc
    16021 tgctgcgcgg tgagagtagc gcggaaagtc gtaacggggc gaaagcgttg ctcgaacagt
    16081 tagggctggg taaacgtctg gatcatcttc cggcacagct ttccggcggt gaacagcaac
    16141 gagtggcgct ggcacgagcc tttaatggtc gacctgatgt gctgtttgcc gacgaaccca
    16201 ccggcaacct tgaccgccag acgggcgata aaattgccga cctgctgttt tccctcaacc
    16261 gtgaacatgg caccacgttg attatggtga cccacgacct gcaactggcg gcacgctgcg
    16321 accgctgctt acggctggtg aacgggcagt tgcaggagga agcatgattg cacgttggtt
    16381 ctggcgcgaa tggcgttcgc cgtcgctatt aattgtctgg ctggcgctaa gcctggcggt
    16441 ggcctgcgtg ctggcgctgg gcaatatcag cgatcgcatg gagaagggct taagccagca
    16501 aagccgtgag tttatggcgg gcgatcgggc gttgcgcagt tcacgcgaag tgccgcaagc
    16561 gtggctggag gaagcgcaaa agcgcggcct gaaagtcggc aagcagctga ctttcgccac
    16621 aatgaccttt gcaggcgaca caccgcagct ggcgaacgtc aaagcggtgg atgatatcta
    16681 cccgatgtat ggcgatctgc aaactaatcc ccctggcctg aaaccgcagg cgggcagcgt
    16741 attgctggcc ccacgcctga tggcactgct taacctgaaa acgggcgaca ccattgacgt
    16801 gggcgatgcc accttgcgga ttgccggaga agtgattcag gaaccggatt ccggttttaa
    16861 ccccttccag atggctccgc gtctgatgat gaatctggcg gatgtcgata aaaccggagc
    16921 cgtgcagccg gggagtcggg tcacctggcg ttataaattc ggcggcaacg agaaccagct
    16981 cgacggctat gagaaatggt tgttacctca gcttaaaccc gaacaacgct ggtacggtct
    17041 ggaacaggac gaaggcgcgc tggggcgatc gatggaacgc tcgcaacagt tcctgctgct
    17101 ttcggcgctt ctgaccttgc tgctggcagt ggcagcggtg gcggtagcga tgaatcatta
    17161 ctgtcgcagt cgctacgatc tggtggcgat cctcaaaacg ctgggggcag ggcgaacgca
    17221 actgcgtaag ctaatcgtcg gtcagtggtt gatggtgctg acgctttcag ccgttaccgg
    17281 tggggccata ggcctgttgt tcgaaaacgt gttgatggtg ctgctcaagc ccgttctgcc
    17341 tgctgcacta ccgccagcca gcctctggcc gtggctgtgg gcgcttggca ccatgacggt
    17401 catctcgctg ctggtggggc tacgaccata ccgcttgttg ctggcaacgc agcctttacg
    17461 cgtattacgt aatgatgtgg tagcgaacgt ctggccgctg aagttttatc tgccgattgt
    17521 cagtatggtg gttgtgctgc tgctcgccgg attaatgggt ggcagcatgc tgctttgggc
    17581 ggtgctggcg ggcgcagtag tactggcttt gctgtgcggt gtgctgggct ggatgcagct
    17641 gaatgtactt tgccgcatga cgctgaaatc gctacctctg cgcctggcgg ttagccgcct
    17701 gttacgtcag ccgtggtcaa cgttaagcca gctttcggca ttttcgctct cctttatgct
    17761 gctggcgctg ttgctggtat tgcgttgcga tctgctcgac cgctggcagc agctaccgcc
    17821 agaaagcccg aactactttt taattaacat cgccacagaa caggtagcac cgctaaaagc
    17881 gttcctcgcg gaacatcata tagtcccgga atcattttat ccggtagtgc gggcgcggct
    17941 gacggcgatt aacgataagc cgacagaagg taatgaagat gaggcgctta atcgcgaact
    18001 caatcttacc tggcaaaata cgcggcccga tcataatccg attgtcgccg gtaactggcc
    18061 gccaaaagcc gatgaagtgt cgatggaaga ggggctggcg aaacgcttaa acgttgccct
    18121 cggtggtacc gtgactttta tgggcgatac ccaggagttc cgcgctaaag tgaccagcct
    18181 gcgcaaagtg gactgggaaa gtctgcggcc taatttctat tttattttcc cggaaggggc
    18241 attagacggg caaccgcaga gctggcttac cagtttccgc tgggagaatg gcaacggcat
    18301 gttgacgcaa ctcaaccgcc agttcccgac cattagcctg ttagatattg gcgcgatttt
    18361 aaaacaggtc ggttaggtgc tggagcaggt aagtcgggcg ctggaggtga tggtggtgct
    18421 ggtcaccgcc tgcggtatgt tgctgttgct ggcacaggtg caggtgggaa tgcgccagcg
    18481 tcatcaggag ctggtggtat ggcgcacact cggtgcgggg aaaaaactgc tgcgcacgac
    18541 attgtggtgt gagttcgcca tgcttgggtt tgtttccggc ctggtggccg caattggtgc
    18601 ggaaacggca ctggcagtgt tgcaggcgaa agtgtttgat ttcccgtggg agccagactg
    18661 gcgattgtgg attgttctgc cgtgcagcgg tgcgctgctg ctgtcgcttt tcggcggctg
    18721 gctgggtgcg cgactggtta agggtaaggc gctgttcagg cagtttgcgg ggtgatgaga
    18781 gtaaataagg ggcgttctgg ttgaatggaa cgccttgttt aaattaattc tacaatatgt
    18841 ttcattgatg atgttattga attggtgatt tcctatcttt ctattgctga tattaatctg
    18901 aattggtgga gtttaataat tttaaatata gccctacaaa aataactcag actaataaaa
    18961 tcataaatca tatgcgttga atggatatta tccatatagt gaatttgttg atgatgaatt
    19021 catctgtgct aaaaatttta gtttaataaa atattgaaag tgacctgtaa taacagttat
    19081 tgttgattga gaacaaataa gtttatgtga aaaatatata aatacattag ctggtcatgt
    19141 gtgtcatttt atttttttgt tgctaacaca gggatatgaa caataactaa aagggcactt
    19201 tatatgagcg gaaaaccagc ggcgcgtcag ggagatatga ctcagtatgg cggtcccatt
    19261 gtccagggtt cggcaggtgt aagaattggc gcgccaactg gcgtggcgtg ctcggtgtgt
    19321 ccgggcggga tgacctcggg caacccggtg aacccgctgc tcggtgcaaa agtacagccc
    19381 ggcgagacgg accttgcgct gcccgacccg ctgccgttca ttctctcccg cacctacagc
    19441 agctaccgga cgaagacgcc tgcgccagtg ggaacttttt ggccccggct ggaaagcgcc
    19501 ttctgatatc cgcttacaga tacgcgatga tgcactggta ctcaacgaca acggcgggcg
    19561 gagcattcac tttgagccgc tgctgccggg gaaggctgtg tacagccgca gtgagtcaat
    19621 gtggctggtg cgtggcggta aggcagcgca gccggacggc cacacgctgg cacgactgtg
    19681 ggcgtcgctg ccgccggata tccggttaag cccgcatctt tacctggcga ccaacagcgc
    19741 acaggggccg tggtggatac tggggtgatc tgagcgggtg ccgggtgctg aggacgtact
    19801 gccagcgccg ctgccgccgt accgggtgct taccgggatg gcggaccgct tcgggcggac
    19861 gctgacgtac cggcgtgagg ccgcaggcga cctggccggg gaaatcaccg gcgtgacgga
    19921 cggtgccggg cgggagttcc gtctggtgct gaccacacag gcgcagcagg cggaagaggc
    19981 ccggcagcag gcggtcgtcg ccggagcaaa acgaccggac attcctgatt cactgccgga
    20041 gagcacagag tacggccccg acaggggtat ccgcctttcg gcggtgtggc tgatgcacga
    20101 cccggcatac ccggagagcc tgcccggtgc gccactggcg cggtacacgt atacggaagc
    20161 cggtgaactg ctggcggtat atgaccgcag caatacgcag gtgcgcgctt tcacgtatga
    20221 cgcgcagcac ccgggccgga tggtggcgca ccgttacgcg ggaaggccgg agatgcgcta
    20281 ccgctacgac gatacggggc gggtggtgga gcaactgaac ccggcagggt taagctaccg
    20341 ctatctttat gagcaggacc gcatcaccgt caccgacagc ctgaaccggc gtgaggtgct
    20401 gcatacagaa ggcggggccg ggctgaaacg ggtggtgaaa aaagaactgg cggacggcag
    20461 cgtcacgcgc agcgggtatg acgcggcagg aaggctcacg gcgcagacgg acgcggcggg
    20521 acggcggaca gagtacggtc tgaatgtggt gtccggcgat atcacggaca tcaccacacc
    20581 ggacgggcgg gagacgaaat tttactataa cgacgggaac cagctgacgg cggtggtgta
    20641 cccggacggg ctggagagca gccgggaata tgatgaaccg ggcaggctgg tatcggagac
    20701 atcgcgcagc ggggagacag tacgctaccg ctacgatgac gcgcacagtg agttaccggc
    20761 gacgacaacg gatgcgacgg gcagcacccg gcagatgacg gcggttcacc gcgaggaagg
    20821 tatcagcctt taccgccact atgacaaccg tggccggtta acctcggtga aagacgcaca
    20881 gggccgtgaa acgcagtatg agtacaacgc cgcaggcgac ctgactgccg ttatcacccc
    20941 ggacggcaac cggagcgaga cacagtacga tgcgtgggga aaggcggtca gcaccacgca
    21001 gggcgggctg acgcgcagta tggagtacga tgctgccgga cgtgtcatca gtctgactaa
    21061 cgagaacggc agtcacagcg acttcagcta cgatgcgctg gaccggctgg tacagcaggg
    21121 cggctttgac gggcggacgc aacgttatca ttatgacctg accggaaaac tcacgcagag
    21181 tgaggatgag gggcttgtca ccctctggca ctacgatgcg tcggaccgca tcacgcaccg
    21241 gacggtgaac ggcgacccgg cagagcagtg gcagtatgac ggccacggct ggctgagaga
    21301 aatcagccac ctgagcgaag gccaccgtgt cgctgtccac tacggctacg acgataaggg
    21361 ccgcctgacg ggcgaacggc agacggtgga gaacccggag acgggggaac tgctgtggca
    21421 gcatgagacg aaacacgcct acaacgaaca ggggctggca aaccgcgtca cgccggacag
    21481 cctgccgccg gtggagtggc tgacctacgg cagcggttac ctggcgggca tgaagctggg
    21541 cgggacgccg ctggtcgagt atacgcggga caggctgcac cgtgagacgg tgcgcagctt
    21601 cggcagcatg gcaggcagta atgccgcata tgaactgacc agcacataca cccccgcagg
    21661 ccagttacag agccagcacc tgaacagcct ggtgtatgac cgtgactacg ggtggaatga
    21721 caacggcgac ctggtgcgca tcagcggtcc gcgacagacg cgggaatacg gctacagcgc
    21781 cacgggcagg ctggagagcg tgcgcaccct cgcaccggac ctggacatcc gcatcccgta
    21841 tgcgaccgac ccggcgggaa accggctgcc ggacccggag ctacacccgg acagcacgct
    21901 cacggcgtgg ccggataacc gcatcgcaga ggatgcgcac tatgtttacc actatgatga
    21961 atacggcaga ctgacggaga agacggaccg catcccggcg ggtgtgatac ggacggacga
    22021 cgagcggacc caccactacc actacgacag ccagcaccgc ctggtgttcc acacgcggat
    22081 acagcatggc gagccactgg tcgagagccg ctacctctac gacccgctga gccgccggac
    22141 ggggaaacgg gtgtggcgtc gcgagcgtga cctgacgggg tggatgtcgc tgtcgcgtaa
    22201 accggaggtg acgtggtacg gatgggacgg cgaccggctg acgacggtac agaccgacac
    22261 cacgcgtatc cagacggtat accagccggg gagcttcgcg ccgctcatcc gcatcgaaac
    22321 agacaacggt gaacgggaga aagcgcagtg ccgcagcctg gcggagaagt tacagcagga
    22381 agggagcgaa gacggtcacg gtgtggtgtt cccggctgaa ctggtggggc tgctggacag
    22441 gctggagggg gaaatccggg caaactgcgt gagcagtgaa agccgccagt ggcttgcgca
    22501 gtgcgggctg acggtggagc ggctggcggc acagatagag ccggtttatt tgcctgagcg
    22561 aaaaatacat ctttatcact gtgaccaccg gggcctgccg ctggcgctta tcagtgaaga
    22621 cggcaatacg gcgtggagcg cggaatatga tgaatggggc aaccagctta atgaggagaa
    22681 cccgcatcac ctgcaccagc cgtaccggct gccggggcag cagtatgata aggagtcggg
    22741 gctgtactac aaccggaacc ggtactacga tccgttgcag gggcggtata tcactcagga
    22801 cccgataggg ctggaggggg gatggagtct gtatgcgtat ccgctgaatc cggtgaatgg
    22861 tattgatcca ttagggttaa gtcccgcaga tgtagcgcta ataagaagaa aagatcaact
    22921 aaaccatcaa a
//
LOCUS       DAJTMC010000055        22865 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.055, whole genome shotgun sequence.
ACCESSION   DAJTMC010000055 DAJTMC010000000
VERSION     DAJTMC010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22865
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.055"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            40..969
                     /gene="aaeR"
                     /locus_tag="OO130_001882"
     CDS             40..969
                     /gene="aaeR"
                     /locus_tag="OO130_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HCR5365319.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            complement(1097..2542)
                     /gene="tldD"
                     /locus_tag="OO130_001883"
     CDS             complement(1097..2542)
                     /gene="tldD"
                     /locus_tag="OO130_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HCR5365320.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVGHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVRGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(2698..6498)
                     /gene="yhdP"
                     /locus_tag="OO130_001884"
     CDS             complement(2698..6498)
                     /gene="yhdP"
                     /locus_tag="OO130_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HCR5365321.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGGNDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFHLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDDVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKLLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPAAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLPVNVKVDGNLSSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDLTISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            complement(6566..8035)
                     /gene="rng"
                     /locus_tag="OO130_001885"
     CDS             complement(6566..8035)
                     /gene="rng"
                     /locus_tag="OO130_001885"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HCR5365322.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            complement(8025..8618)
                     /gene="yhdE"
                     /locus_tag="OO130_001886"
     CDS             complement(8025..8618)
                     /gene="yhdE"
                     /locus_tag="OO130_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HCR5365323.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAHKKAQAGVAQTAQDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVVLADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            complement(8627..9115)
                     /gene="mreD"
                     /locus_tag="OO130_001887"
     CDS             complement(8627..9115)
                     /gene="mreD"
                     /locus_tag="OO130_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HCR5365324.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            complement(9115..10200)
                     /gene="mreC"
                     /locus_tag="OO130_001888"
     CDS             complement(9115..10200)
                     /gene="mreC"
                     /locus_tag="OO130_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HCR5365325.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPTTGIAQPTPQQPTTGNAATA
                     PAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            complement(10266..11309)
                     /gene="mreB"
                     /locus_tag="OO130_001889"
     CDS             complement(10266..11309)
                     /gene="mreB"
                     /locus_tag="OO130_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HCR5365326.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNN
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            complement(11614..13554)
                     /gene="csrD"
                     /locus_tag="OO130_001890"
     CDS             complement(11614..13554)
                     /gene="csrD"
                     /locus_tag="OO130_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HCR5365327.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSTLRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGSSD
                     LFRELSVPLIKYPGMSLRLVYQDPMGNYFHSLMTTAPLTGAISFIIVMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIAGQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTQVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            13706..14680
                     /locus_tag="OO130_001891"
     CDS             13706..14680
                     /locus_tag="OO130_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR5365328.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVMVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAIIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            15658..16128
                     /gene="accB"
                     /locus_tag="OO130_001892"
                     /gene_synonym="fabE"
     CDS             15658..16128
                     /gene="accB"
                     /locus_tag="OO130_001892"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HCR5365329.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            16139..17488
                     /gene="accC"
                     /locus_tag="OO130_001893"
     CDS             16139..17488
                     /gene="accC"
                     /locus_tag="OO130_001893"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HCR5365330.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(17580..18627)
                     /locus_tag="OO130_001894"
                     /pseudo
     CDS             complement(17580..18627)
                     /locus_tag="OO130_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787050.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
     gene            complement(18624..19586)
                     /locus_tag="OO130_001895"
     CDS             complement(18624..19586)
                     /locus_tag="OO130_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCR5365331.1"
                     /translation="MSKVFTIGEILVEIMASKIGQPFDQPGIWNGPYPSGAPAIFIDQ
                     VTRLGVPCGIISCVGNDGFGDINIHRLAADGVDIRGISVLPLEATGSAFVTYHNSGDR
                     DFIFNIKNAACGKLSAQHVDENILKDCTHFHIMGSSLFSFHMVDAVKKAVTIVKANGG
                     VISFDPNIRKEMLDIPEMRDALHFVLELTDIYMPSEGEVLLLSPHSTPERAIAGFLEE
                     GVKEVIVKRGNQGASYYSANEQFHVESYPVEEVDPTGAGDCFGGAWIACRQLGFDVHR
                     ALQYANACGALAVTRRGPMEGTSRLAEIEAFIQRHDMSIREAAQ"
     gene            complement(19608..20597)
                     /locus_tag="OO130_001896"
                     /pseudo
     CDS             complement(19608..20597)
                     /locus_tag="OO130_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525780.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(20598..22097)
                     /locus_tag="OO130_001897"
     CDS             complement(20598..22097)
                     /locus_tag="OO130_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCR5365332.1"
                     /translation="MTDVILDVSHIAKTFGHVQALKDITLSLRKGRVHTLLGENGAGK
                     STLMKILAGVYPPTQGTITLRGETITINNPQHSRQLGIAIIFQELSLSNNMTVAENIY
                     ANNEPRRFGIINDKKMLADCQNLLAERGIPLDPLEMVGNMSMAHRQLVEIAKALSYAA
                     DVVIMDEPTSSLSDNEAEILFNIIEKLKQRGCAVIYISHRMDEIMRISDDISVIRDGE
                     YIATHEKKNSDIQHLIAQMVGREMKNIWPARLGEIPDENVPAKLEVKNLSHPSLFKEI
                     SFAVRPGEVLGFFGLVGAGRSDVMKALFGLVSYQGTVLIDGKEVRITNPKQAIDHGIA
                     FVTENRKEEGLVLMHDVNMNTHHVAFQYNASRMGLINHRQEEAKTLQSIARMNTKVSS
                     VHQAVGALSGGNQQKIVLSKWLEKTPRILLLDEPTRGVDVGAKFEIYNVIRQLAAAGT
                     AIILVSSELPEVMALSDRLVVMRNKTIADIYSCENLTQTQVMTAATGVR"
     gene            complement(22158..>22865)
                     /locus_tag="OO130_001898"
     CDS             complement(22158..>22865)
                     /locus_tag="OO130_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCR5365333.1"
                     /translation="LFANQDLNKQLSDVEDFITKKVDAIIISPVDSKGVQAAIIKADK
                     AGIPVITVDVAAEGVPVVSHVATDNYAGGVEAGKLMGKLLNGKGTVAIISYPALQSVV
                     ARVDGFKKGLSDTPDIKIVAEQPSITRAEALTTAQNIMQANANLNGLFGFGDDAALAA
                     VIAAKSAHNDNIKIIGFDGMKEARDAVDSEKTFAAVIRQYPDQMGAKAIDAAVDHLNG
                     KPVEKMIPVQPGVYTGK"
BASE COUNT         5669 a         6192 c         5460 g         5544 t
ORIGIN      
        1 tattatgtga tctaaatcac ttttaagtca gagtgaataa tggaacgact aaaacgcatg
       61 tcggtgtttg ccaaagtagt tgaatttggc tcttttaccg ccgccgccag acagctacag
      121 atgagcgttt cgtccatcag tcagacggtg tcaaaactgg aagatgagtt gcaggtaaag
      181 ctgttaaacc gtagcacacg cagcattggc ctgaccgaag ccggtagaat ttactaccag
      241 ggctgccgtc gtatgcttca tgaagtgcag gatgttcatg agcaactgta tgctttcaat
      301 aacaccccca tcgggacgct acgcattggc tgttcttcaa ctatggcaca aaatgttctc
      361 gccgggctga cagccaaaat gctgaaagaa tacccaggtt tgagcgtcaa tctggttacc
      421 ggaattccag cccccgacct gattgccgac ggtctggatg tggtgatccg cgtcggcgcg
      481 ttgcaggatt ccagcctgtt ttcccgccgt cttggcgcga tgccaatggt ggtgtgcgcc
      541 gcgaaaagct atctcacaca atacggcata ccggaaaaac ccgccgattt aagtagccat
      601 tcatggcttg aatacagcgt gcggcccgac aatgaatttg aactgattgc accggaaggg
      661 atctcgactc gcctgatccc gcaagggaga tttgtgacta atgatccgat gacgctggtg
      721 cgctggctaa cggcgggtgc cgggatcgcc tacgtgccgc tgatgtgggt gatcaacgag
      781 atcaatcgtg gggagctgga gatcctgctg ccgcgttatc agtcagatcc acgcccggtt
      841 tatgcgttat ataccgaaaa agataagctg ccgctgaagg tacaggtcgt gatcaactcg
      901 ctgacggatt attttgttga ggtcggtaaa ttgtttcagg agatgcacgg gcgcgggaaa
      961 gagaagtaat tcatattgta ctgttacgtt ctacaaacct gtgccaacgg gttcccctca
     1021 ccctaacccc agaggagcga ggggaccgtt cgtgcacgta gaaagattaa ttatccttct
     1081 gaaaataatg aaattattac gcagtaccgc caacagtcag gttatcaact ttcagcgttg
     1141 gctggcccac gccaaccggc aaactttgcc cttctttacc gcagacaccc acgccgttat
     1201 ccagtttcag gtcgttgcca accatcgaaa tctgctgcat ggtttcgata ccggaaccaa
     1261 tcaacgttgc gcctctcacc ggcttcgtta ctttaccgtt ttcaatcaga tatgcttctg
     1321 aagtggagaa aacgaatttg ccggaggtga tatccacctg accgccacca aagttcggtg
     1381 catagatacc gtactcaacg gattcaataa tttcctgcgg ggtcgattta cccggcagca
     1441 tataggtgtt ggtcatacgc ggcatgggca gatgggcgta ggattcacgg cgaccgttgc
     1501 cagtcggcgt catccccatc aaacgcgcgt tgagtttatc ctgcatgtag cctttcagaa
     1561 tgccgttctc aatcagcacg ttgtactggc ctggcgtacc ttcgtcatca atcgccaccg
     1621 aaccacggcg atcgaccatc gtgccgtcat caaccacggt acacagttct gaagccacca
     1681 gctccccgac ctgcccacta aatactgaag tgccgcggcg gttgaagtcg ccttccagac
     1741 cgtgacctac cgcttcatgc aacagcacgc ccggccaacc tgcgccaagt actaccggca
     1801 tggtgcccgc tggtgcagca acggcagaaa gattgaccag cgccatacgc actgcttctt
     1861 ttgcccatgc atccgcacgg acttcgccgt caagatcggc aaggaagaac tcataaccaa
     1921 aacgaccgcc accgccactg gcaccgcgtt cgcgtttgcc atcttcttcg accagaacgc
     1981 tcacggaaag acgcaccagc ggacggacat ccgccgctag cgtgccgtcg gtggccgcaa
     2041 ccagaattaa ttcatagaca ccactgaggc tggcagtcac ttcctgtacg cgcttgtccg
     2101 cttcgcgagc aaccttatcg acgcgacgca ggatatccag cttctcttca cggctcatgc
     2161 tttgcagcgg atctaccgag gtatacaacg ggctatgccc taccgcgccc agcgtctgta
     2221 ctttgccatc accactatca cggacgatgg tgcgcgccgc ttgcgcactc tgttccagcg
     2281 ccagcaggct gatttggtca gcgtaagcaa atccggtttt ttcaccgctg attgcacgca
     2341 cgccaacgcc ctgatcgatg ttgtaagaac catctttaat aatgcggtct tctaaaaccc
     2401 aggattcgtg atagctcgac tgaaaataga gatcgccata atcaaggcga cgttcggcca
     2461 gttgaccgag gatcgcgaac aagtcctgat gtttcaggcc gttcgccgct agcaattgtt
     2521 cacttactag gttaagactc atcgtttttg ctactcgtta gttactgcag tagaggattt
     2581 tttacggctg ccggatgcgg cgtgaacgcc ctatccgacc tacggttatg ttctggctcg
     2641 ttggcctttg gcaacgatta tcatatgaga ttggggcaat tacgcgccct cgtcaaatca
     2701 ttgcgctttt tctttacgcg gttggcgcaa cacttcgttg atttgcggat cgtccagcgg
     2761 acccgaaatg tgatagcgca aaatggagac tttgctccac agcggcccca gcactttact
     2821 ggctgcaaat actgccgcgc caacaatggg gttaaccgca aaagccgcag ccacgcccac
     2881 cgtcgcagaa atctctggtg cgacaaccgc ttccatattc aggtcgcgac gtaccagatt
     2941 taccgaccct ttcatggcga tatccgcctc caggccatcc accagcgtgt cgtcggtgtg
     3001 cataacgccg tctttaatcc acgcggtgct gcgaatggag tcaaaataga acccttcgcc
     3061 aaaagtgtct ctgaaatcaa aacgcagctt acgcatcagg gcatcaacgc tcaataagcg
     3121 cagcaattgc ccggcatgtc cggtattgat ttcggtaatt tcgcctttac ccagttgagt
     3181 atgaatgatg ccattcaacg tcgcctcatc tggttgccag ggtgctttgc gccagtgtaa
     3241 atcgtaatcc acattaaatg acgactggcg tatgggcgtc gtgacaccaa aaaattctgc
     3301 ggcggcatca attttctgcc cgcgcagttt tcctttcagc gaggtacgtt cattccccgg
     3361 attattaacc cattcaccat cggcagtaag ccgcgagaaa ccagtatcaa tcagtccatt
     3421 ggtcagcgtt aacgtatcgc cagaaatggt gagatcactg tcaatacgac cgaatttttg
     3481 cccccagaac cagcactctg tgcatcgtat ttgtgcgtcc ggccagccac ggaagttaat
     3541 gcgctccgtt gtcgggaaag gtgatgacgg tgttgaatca ccacgagttt tcgccacgct
     3601 cgggttgtaa taaagatatt tgatattcgc cagccacggc gcgttattac gcatcgccag
     3661 cgtggcattg atttcacgcc cctgcgcctc aaccagggtg ccatttgccg tcggttgcga
     3721 aacaatactc aggttattcc attgctgatt tcccagtgac aacataggcg tacgtaacgt
     3781 tatgtgttgt gggaaactcg ctgcaccacc gacactctcc gcagcgcctt tctggaacag
     3841 ggccagccac tcggcaccat tcatcggcgg catattgagt tcaacgccac tttgttccgg
     3901 caacggcggg agcgttttac tgtctgccgc ccaaatagca cgatcgagcg tcagcttttg
     3961 accaagcaac cagcggctat tgaaatggtt atccgcacca gcctgtccgg ttaattcaaa
     4021 gctgctgaga ttgccatcaa ccttaacgtt taccggcagt ggttcacccg caggtttggc
     4081 taacggtgaa ggtaagtgac tgctcacatt attcagatcg ccgttcagct ctacgttata
     4141 ggtcgcgcca gcatgataag gcagatcaat gcccacttta ccatcccacg ccacgctgcc
     4201 actcaatgct tcgttcaccg ctgcaggcag aacgccggtt ttcgccggtt gccagttacc
     4261 gttgaggttt accgcaacct ggtaggcttt tgccccttct ttggtggaaa aatccacgtt
     4321 caacggctga ttaaaccagc ttgctgtcag tggttcactt tgcagatcgc cattgataaa
     4381 gctgaattta ccgctcaaat ttttcagggt gctgtcgagt agtttgataa acagactgtt
     4441 attacgcagc gtcacttcac ctttcgcggt taccagttcg ccgttcagcg ggatatcaag
     4501 atgtaagcga gcattcacat cgccgtcgag ctggagttct tgcagggtcg cacccagaga
     4561 atctttcagc ggtgtctcat caaagtaagg gccaacggct ttacccggac ctttaatgtc
     4621 agcgtcaatc agcagttttt ctttcgagta gtcagggatc actgcggtga gattactcgc
     4681 gcgcacgccg cccagattaa cgtcatcggt tttcatccat aaaccgtcgt taataaagtc
     4741 cagttcaata tcaaggttag ttaatgcagg ccagtccggc tggaaggcaa acttcgcgtt
     4801 acgcagcggc accagcactt caaactgacc ttcgttgtgt ttatagggga agagttgcgg
     4861 attgccacca taaaccagcg tcgcgttatc cgcttcaccg ccctgaatcg cgccacttaa
     4921 gtaatcaact aggtctttac ccatcaagtt ttccgggaaa tagcgccagg cttgtgaacc
     4981 atcatcggta ctgatgccag ccagaatacc cagccagggt tcatcgttag caggttgcag
     5041 gtaacgaaaa ccgccgcgcg catggacagc tttggcttta acgtcaatat tacgcccatc
     5101 caggtggaaa cctttgtcat tgttcagcca gcttatagtt gcctggccgt cggcgatttc
     5161 cagtggcgca cggaataccg tttcgtaagg catctttgcc tgcttcatcg acgcggtaag
     5221 caaaccattt tcaacgctgc cggaaagcgt cccggagaag tgttccgcac ccggtaataa
     5281 tttccattgc ttccaggcca gatcgctcca cgatgcctga aaacgggtct tgtctgccgc
     5341 ctgaagcggg atatccagcg ccagagtgtt aatcttgccg ctcggttgtg tggagcgcca
     5401 aacatcaccc agtgcaggtg aaagtttcgc ggccagcggg cgtatgccct ccaggcctgc
     5461 cagctccaga ttactggcgc gaatccggag ttcgtcactg cgtttattgt ctttgccgcc
     5521 aacgtcctgt tccggtatcc aggccagcgt caatgctccg ctcggccagg gtttgccgtc
     5581 catcgtgatc cgtgtatcgg gaatagagaa ctgccagccc ggattttcac gcgtaatatg
     5641 cgcggtcaga ttatccaccg acagcgtatg cgtttgcttc tcgcccaacc agctggcacc
     5701 gccctgtttc agccagacgt caccgccggt tacatcgcct ttgtcgatcg tcatccagcc
     5761 ttcaagggag aactgtgccg tttccagagc aatattgtcc tgcatccatt taccgagcca
     5821 cggcttcagg tcgatgtcat ccgcctgtag ccagacgcga ccattgctta acaacccctc
     5881 atcatcgcgc aaatccatgc gcacctgcat cacgccgtgc tgtccggtaa ggctggagag
     5941 gcttaccagg ccttccgcac ggtgtcgacg tggatcgttc agccaggtga gttgtgggat
     6001 cgccagctcg gcgcgctgac cggatggcgt cagaaaactg acttcactgt cgcggagatc
     6061 gaaatggtca aattgacgaa gaaacagatc gctgatgtga ctggcttcca ggctatcatt
     6121 accaccaccg ctggtgatag gagtgttggt gcgaaagcgc agctgccaga aagtgaggtc
     6181 gcgaaactgc cagcgcatat gtaacaagct ctgccagaca tccagcgcca gagtaacgcg
     6241 tttaaccgaa aattcgccgc catcttttag ttctgcacgg atgtcgtgtg cttcaagcgt
     6301 cgggccaaaa ttctgccagc tggctgagag ctgactggct tctaccggca tgccagtcgc
     6361 ggattctatt ttgttgagga tttccggacg ccaggcgtca agatgcggta aagcaatacg
     6421 caggccgcta accagcaggg ctgcgatcac aacgagcgcg gctccagtaa gcagtaaaat
     6481 ccccggcaat cgcctcaccc gtcactcctt gtctgctaaa aatgtgactc aaaaaccctt
     6541 tgccggatgg cggcccagca tctgtttaca tcattacgac gtcaaactgc tcctggttat
     6601 agagcggttc aatttgtact ttaacctgtt tgccaacgaa aatttccact tccgccagcg
     6661 agtgtgactc ttcgcctttc aaggcttcag ctactgccgg agaagcatag accaggaaac
     6721 ggtcggagtc gtaagcatgg tggacacgaa caatctcgcg catgatttca tagcataccg
     6781 tttccacggt tttcaccgtt ccgcgaccgt ggcaggttgg gcattcgtta cacagtacgt
     6841 gctcaatgct ttcgcgggtg cgtttacgcg tcatctccac cagccccagc gccgaaaaac
     6901 cattaacgct ggttttcacc cggtctttgc tcaacgcctg ctccagcgag tgcagcacgc
     6961 ggcggcggtg atcttcatta ttcatatcga tgaaatcaat aatgataatc ccacccagat
     7021 tacgcaaccg taactggcga gcgatagcct gcgtcgcttc aatattggta ttgaaaatgg
     7081 tgtcgtccag attgcgatga ccgacaaacg ctccggtatt gatgtccacg gtggtcatcg
     7141 cttcggtctg gtcgataatc agataaccac cggatttcag ttctactttg cgttccagcg
     7201 ctcgctggat ttcgttttcg acatcaaaga gatcgaaaat cggctggcgt ccggtgtaat
     7261 gctccagctt gctggtcatc tcgggaatgt actccgaggt aaactccagc aatgcttcgt
     7321 aagtcaggcg tgagtcaacg cgaatgcggt ccagttcggc gtcggcgaaa tcacgcagaa
     7381 cacgctgcgc cagcgccagt tcgccgtaca gctgataacg ggtctgcggg cgttttttac
     7441 gctccataac tttggtccag acgcgtttca gataagcggc atcggaggcc agttcagcct
     7501 cgccaacccc ttccgctgcg gtacggatga taaacccgcc ctgctcgtcg caatactctg
     7561 cgaccacttt tttcaggcgt tcacgttctg attcgctttc aatacgttgg gaaaccccaa
     7621 cgtgagaagc ccctggcata aacaccagat agcgagaagg cagcgtgata tcggtggtca
     7681 ggcgcgcacc tttagtgcca agcggatctt tcaccacctg caccatcaga tcttgcccct
     7741 gacgaaccag ttccgagatg tcgcgcaccg tgaattgctt ttgttcttca cccgccacac
     7801 attcggtgtg cggcatgata tcggatgcat gaagaaacgc ggctttatcc agcccaatat
     7861 ctacaaaagc cgcctgcata cccggaagta cacgacttac acgacccttg tagatattgc
     7921 ctactattcc gcgtcgcgcc tcacgttcaa tatgaatttc ctgcagaata ccgccatcaa
     7981 tatacgccac tcgcgtttcc gaaggcgtta cgtttactaa caattcagcc gtcatgttta
     8041 tcccttttct cacgcagtgc gttaaaatta cttaataatt catacgtttc aaccagcggt
     8101 aagccgacta cggcgtgata gctgccattt atcttcctga caaaacagcc acccagcccc
     8161 tgaataccgt atgcacctgc tttatctaac ggttcaccgc tggcgacata gcccgcgatg
     8221 tcttcgtctg ttaacgttct gaaagtcaca tcggtgacca ccaggcaatc gagaatgtgc
     8281 tggctgtcgg ccaacaccac tgccgtcatc acctgatggg tctgacccga taatttgcgc
     8341 aacatctgcg ccgcatgctc tgcgtcgcgc ggtttctcca gcacttctcc attcaggata
     8401 acgatagtat ccgcacccag caccgggaga tcctgcgccg tttgcgcgac acccgcctgt
     8461 gctttcttgt gtgccagacg cacaacatac tgctgcgcgc tctcctgcgg ctgacgctgc
     8521 tcctcaatgc ccgtaacaat acgttcaaag gtcacgccaa gttgcgcaag taactcctga
     8581 cgacgcggag aaccggaagc taaatacaga gaagtcatag aaacctttat tgcactgcaa
     8641 actgctgacg gactttacgc atcagcaaga aaatccacgg ccagagcacc ccattgacta
     8701 cactactcca gaacacttcc ggtctgaaag agacgttaat cactaaaaac tctgcccaga
     8761 aaacaataat atccaccacc agcgaaagca acatgacgac tagcgcctgc tgccataatg
     8821 cgaggttgcg gaaaagctgg tatttcagcg ccaccaggta agcaatgatg ctcatcgcca
     8881 atacgcgtac gccaagcgtc gagccgctga tcagatccag tatggcaccc atcacaaaac
     8941 ctgtgcccac atttacgcga tggggcaagg ccaggatcca atacaacaag ataagtaaca
     9001 cccagtttgg ccggaaaaca atcaggttat ccggccaggg catgatttgc agcaacagcg
     9061 caatgaggaa agagagccag attacccaac gtccctggct acgatagctc gccactattg
     9121 ccctcccggc gcacgcgcag gcggttgagc accactttgc ggcggcgtag ccctttgtgg
     9181 agagcgatta gcagcaggcg cagtagctgc atttcctgtc gtcggttgct gcggagtcgg
     9241 ctgagcgatc cccgttgtcg gttcaggtaa ctttggcccc atcgcgtctg gcgaaggcaa
     9301 tacctgcggc atcatctgca tcagacgttc attagcaaca cgatgcacct cttccggcgt
     9361 catcgggtta gcgccgttac gatccgcgcc ccacagcagc agcagataac gcaaacgttg
     9421 cagtcctgca gtcggacgcg cctgaatcac agtataagcg cgctgggtat cgagttttac
     9481 ggaagagaca accgcgaccg gatagccttc cgggaaacga ccgcccagac cggaggtcac
     9541 cagcacatca ccaacacgaa tatccgtatt cgctggcaga tgctcaagct gcaaatcatc
     9601 tgtacaaccg ttaccggctg caattacgcg gatatcgttg cgcagcacct ggattggcag
     9661 cgcgtgggtc gcatcacaaa tcagcagcac gcgactggtc agtttagcga cggccaccac
     9721 ctgaccaaca acacctttgt cgctgatgac cggctggcct tcataaacgc cattaacgct
     9781 acctttatcg ataacaactt gatcgctata aggatcgtta accgtggaga taacctgagt
     9841 caccattttc tgctcatcct gacgcagcgg ggaacccagc agctcgcgca gacgcgcgtt
     9901 ctcctgtttg tattgtccaa gcatcagcag ttcactgttt ttcagcaaca gttcctgacg
     9961 taacgcccgg ttttcaagtt ctagttggtc acgcgaggcc agcgtctgcg atacgccatc
    10021 cagcaattca cgaggagcat tggaaacaaa gtagaaagga ctgacggcgg tatccatata
    10081 agtacggatt tgactgaacg tccccaggcg gctgtcggca ataataatgc cgagcgccac
    10141 cagcaccgcc agaataaggc gaatctgtag cgacgggcca cggctaaaaa ttggcttcat
    10201 aagttatgcg tattctcgta tcagactagg cagggtaaac agacatttcc cctgcctgca
    10261 tccgattact cttcgctgaa caggtcgccg ccgtgcatgt cgatcatttc cagcgctttg
    10321 ccgccaccgc gcgccacaca ggtcagcggg tcttcagcaa caacgactgg aatgccggtt
    10381 tcttccatta acaaacggtc aaggttacgc agcagtgcgc caccaccggt gagcaccatg
    10441 ccgcgctcgg agatgtcgga agccagttcc ggcgggcact gttccagtgc aaccattacc
    10501 gcgctcacaa taccggtcag cggttcctgc agtgcttcga ggatttcgtt ggagttcagg
    10561 gtaaaaccgc gtggaacacc ttctgccagg ttacggccac gaacttcgat ttcacggact
    10621 tcatcgcccg ggtaagccga accgatttcg tgcttgatac gttctgcggt ggcttcaccg
    10681 atcagagaac cgtaattacg acgcacatag ttgatgatag cttcgtcgaa acgatcacca
    10741 ccaatgcgca cggaagagga gtaaaccacg ccgttcaagg agataacagc aacttcagtg
    10801 gtaccaccac cgatatcaac caccatagaa ccggtcgctt cagaaaccgg caggccagca
    10861 ccaattgcgg cagccatcgg ttcttcaatc aggaagactt cacgggcacc agcgccctgc
    10921 gcggattcac gaattgcgcg gcgttcaacc tgggtcgcgc caaccggtac acaaaccaga
    10981 acgcgcgggc ttggacgcat aaagttgttg ctgtgcactt gtttgatgaa gtgctggagc
    11041 attttttcag tcacgaagaa gtcggcgata acgccgtctt tcattgggcg aatggcagca
    11101 atattgcccg gcgtacggcc cagcatctgc ttcgcgtcat gacctactgc agctacgctt
    11161 ttcggtgaac cggcacgatc ctgacgaatg gccaccacgg aaggctcatt caatacgatg
    11221 ccttgtcctt ttacataaat gagggtattc gcagtaccca ggtcaatgga caagtcattg
    11281 gaaaacatgc cacgaaattt tttcaacata ctaagggata atcctgaaag ctggggcgga
    11341 aaagaaaatc cgcttacttt accaaccaca cgcagcagcg acaaggcgca aaaatcatct
    11401 gctacggtga aaattagtgc agttcgtttc ctttgttaca aatctctgcc tgagtccaga
    11461 aaggcttaat gcatcagcag cattcctcgc ctgtttgcaa ccgcgtaagg tcattcatct
    11521 gcatatgtgc tgcaacaatc tggcgagcag acaagcacac tcccatgaga cgcagcgcgc
    11581 gttattctac gtgaaaacgg attaaacggc aggttaaacc gagtatcttt gtgaatattt
    11641 tttcacgtta gtatcaagtg gctgtgagga cgcgaaaaaa tccccttgcc cgcctgtaac
    11701 accgcgctga atcagggtct gccactcgct tcgcgaacgc acaccggtgg cgtaaacctg
    11761 ggtgctggtc ccggagcagg cttccaccag gctttgaacc agcagctggt tctccgttcg
    11821 cttctcaatg tttctgacca gccccggatg gagcttgagt aactcaacat taagttcttt
    11881 gatccaactg gtgcttacca gcgtcaaacc agcctggttg acggctaccc gtacccctaa
    11941 agcattcact aaacgaataa caggttgtaa acgactgata tgttgaccta catcggcctc
    12001 tgcaagttca ataattatgc gttttcgttg tgatttttca cattgcatta acgtatcgcg
    12061 cagccaacgc tgaaaacgcg ggcgaatcag cgactcaacg gtaacctgaa tcgccagatt
    12121 ttcctctggc cagtaacgca atagtggaat aagacggctg atttgcagac ggtcatactc
    12181 ttccgataag ccaaactgca agaccatcgg catatactcc gccgagctaa cctcttcatt
    12241 accatcgaag atgcggcaca tgagttcgcg atgatgaacc tgaccttcgc gagtaaccgc
    12301 cggtttttga taaaggcgcg ggccgccgcg actgagcatt tgctcgataa gcgtacgcca
    12361 gcgaacatta ccgcgtcctt tttcaggcaa cgagtcatcg taaatagccc agctattgcc
    12421 gccctgcaat cccgcattac gcgtggcaga ctctgcatgt tccattacct gctcggtatc
    12481 ctgaccacta cgccaggcgc agataccaat gtggatcata tcgtcgcgat cgagcatttt
    12541 attgttcggc aaggtatcga cggctttgat taactgaccg gcgatgctct ctgcctcttt
    12601 taacgtccgg tgcggtaaca gcgcagcaaa atcactgcgg tggtaacgcg ccagcagtgc
    12661 gccagggtag cgcatcataa atgtcgacag cagattcgtc agagtgaaga actgttcttc
    12721 aacctggctg tgcccccaag tatcgctcaa catattgaaa tccggcagac gaatcatcat
    12781 cacgatcccg tgggtaccta ctttctcctg atcttccagt aacgttgcta actgattatc
    12841 gaaaaagagt cggttattga ggccggtttt cacgtcctgg gcggcataag agcggatcag
    12901 cgtatcaaga cggctgtgtt gttcgcgtgc gttctgaatt tcacgaagca gcgtatccag
    12961 cgcactgctg gttctgggcg gccattcata gatggttccc aacacattag agccacgctc
    13021 accgtttaag atacgagtag cccgggtttc cagcaattct tgcccggcaa gttgccgttg
    13081 taaccagcgt accgccaaga agagcataac aatgataaag ctaatcgccc ccgtgagcgg
    13141 cgcggtggtc atcaacgaat ggaaatagtt gcccatcgga tcctgataaa ccagacgcag
    13201 cgacatcccc ggatacttta tcaacggaac gctcagttca cgaaacagat cgctggagcc
    13261 aaccggacga taactaccat ttctggccag ggtataaacc tgtttatcac catggagcag
    13321 gtctacacga acgatatctg ccgacatcat taattcggta atttgtggcc ttaatgtgct
    13381 gaagtcattc gacacaaggt gggtatcaat cgccgtcgcc accgcctgaa cgcgatgact
    13441 aaacttatac tgaattgcgt tgtagaaact tagcgaacag cccagcaaag tcacaaaaat
    13501 tgttaacccg gtgagcagcg taacaaaggc cgaaaatttc gtcgttaatc tcatccttgt
    13561 gttaactccg atagtgagga agcgggcata ctagcaaatc agatttatct cgcaatttat
    13621 tgcgcttcat cggctttgct tttccattag cgagtataat cttcagaaat tattttccaa
    13681 tccatcatgc acatgaggac cacttatgca ggcgttactt ttagaacagc aggacggcaa
    13741 aactctcgca tcagtacaga ctctggacga aagtcgcctg ccggagggcg atgtcatggt
    13801 cgatgttcac tggtcgagcc tgaactataa agatgcgctg gcgattaccg gtaagggaaa
    13861 aatcatccgt aattttccga tgattcctgg gatcgatttt gccggaactg tacgcaccag
    13921 cgaagatccg cgttttcatg ccggtcagga ggtgttactc actggctggg gcgttggtga
    13981 aaaccactgg ggtgggctgg cggagcaggc gcgagtgaaa ggtgactggc tggttgccat
    14041 gccgcaaggg ctggacgcgc gtaaagcaat aattatcggt actgccggtt ttaccgccat
    14101 gctgtgtgtg atggcgctgg aagatgccgg tgttcgcccg caggacgggg agattgtcgt
    14161 gacgggtgcc agtggtggcg tcggcagtac cgccgtggcg ctgctgcata agttgggtta
    14221 tcaggtcgtt gccgtttccg gtcgcgaaag tacccatgaa tatctgaaaa gtttaggtgc
    14281 cagccgtatt ctccctcgtg atgagtttgc cgaatcccgt cctctggaaa aacaagtctg
    14341 ggctggggca attgacactg ttggcgacaa agtgctggca aaagtgctgg cgcaaatgaa
    14401 ttacggcggc tgcgtggcgg cctgtggtct ggcgggtggt tttactctgc caaccacggt
    14461 catgccattt attctgcgta atgtccgttt gcaaggggtg gattcagtaa tgacgccacc
    14521 agaacgccgc gcacaagcct ggcagcgact ggtcgccgat ttaccggaat cattctatac
    14581 ccaggcggca aaagagatat ctctgtcaga ggcaccgaac tttgccgagg ccatcattaa
    14641 taaccagatc cagggtcgca cgctggtgaa ggttaactaa ccatttagca aggaataata
    14701 agagagggaa ctcattttga catcattatt caatcggtta acgggaaaag cggttagccg
    14761 gacagctttc gtcgaacacc ttggtcagga agttgtacaa catcatccaa actggaaagt
    14821 catgatttcg actgaccaca aattgatgcg cattgatact cctctaaaca gccattattg
    14881 atacgcctcc gtcgcctgtt aggtttatgg tgctttgcct gggcgacact acgcttaacc
    14941 tgttactgat ttctggtacc atggggtgaa taatctgatt ttatttgact gcaaattaac
    15001 cactcgaacc tatttaatgc tgagaattgt cagtcggttg attttgcgtg ctttagcatt
    15061 cacatctatc cagacgatgc agtgaaaatt gggtaatccc cagcaaccgc tgcgtaatgt
    15121 cgtctatctt gtcgcgatcc tggcatccct acattatttg tggtctgtga agattatctc
    15181 attgcagccc ctcatcttcg cagggctggc tttacagctt ttcaccttac gttataagaa
    15241 gttccgtcga tgatggcgct aatttcgtga attgtgcggc ttgttgcaaa ttacacggtg
    15301 ttgaaggtta tttacatgtt agctgttgat tatcttccct gataagacca gtatttagct
    15361 gccaattgct acgaaatcgt tataatgtgc gacctcgtcc tccctgacgc agtttttgcg
    15421 ctgcggaaaa ggtgacattg gcgcaacgaa ggtatatttt gttttttgcc ggaggatagc
    15481 agcagatcgc tgcacaatgt ccgtcaagtc taacattgat actctggggc aaaatagacc
    15541 ggcgtcccgg cctgctggaa tttatcgcta tgcatacagc tgtcggggca tacgctttac
    15601 agacggcggt gaaacgcctg tcacaatcac actaaacaaa gagtacggaa cccactcatg
    15661 gatattcgta agattaaaaa actgatcgag ctggttgaag aatcaggcat ctccgaactg
    15721 gaaatttctg aaggcgaaga gtcagtacgc attagccgtg cagctcctgc cgcaagtttc
    15781 cctgtgatgc aacaagctta cgctgcacca atgatgcagc agccagctca atctaacgca
    15841 gccgctccgg cgaccgttcc ttccatggaa gcgccagcag cagcggaaat cagtggtcac
    15901 atcgtacgct ccccgatggt tggtactttc taccgcaccc caagcccgga cgcaaaagcg
    15961 ttcatcgaag tgggtcagaa agtcaacgtg ggcgataccc tgtgcatcgt tgaagccatg
    16021 aaaatgatga accagatcga agcggacaaa tccggtaccg tgaaagcaat tctggtcgaa
    16081 agtggacaac cggtagaatt tgacgagccg ctggtcgtca tcgagtaacg aggcgaacat
    16141 gctggataaa attgttattg ccaaccgcgg cgagattgca ttgcgtattc ttcgtgcctg
    16201 taaagaactg ggcatcaaga ctgtcgctgt gcactccagc gcggatcgcg atctaaaaca
    16261 cgtattactg gcagatgaaa cggtctgtat tggccctgct ccgtcagtaa aaagttatct
    16321 gaacatcccg gcaatcatca gcgccgctga aatcaccggc gcagtagcaa tccatccggg
    16381 ttacggcttc ctctccgaga acgccaactt tgccgagcag gttgaacgct ccggctttat
    16441 cttcattggc ccgaaagcag aaaccattcg cctgatgggc gacaaagtat ccgcaatcgc
    16501 ggcgatgaaa aaagcgggcg tcccttgcgt accgggttct gacggcccgc tgggcgacga
    16561 tatggataaa aaccgtgcca ttgctaaacg cattggttat ccggtgatta tcaaagcctc
    16621 cggcggcggt ggtggtcgcg gtatgcgcgt agtgcgcggc gacgctgaac tggcacaatc
    16681 catctccatg acccgtgcgg aagcgaaagc tgctttcagc aacgatatgg tttacatgga
    16741 gaaatacctg gaaaatcctc gccacgtcga gattcaggta ctggctgacg gtcagggcaa
    16801 cgctatctat ctggcggaac gtgactgctc catgcagcgc cgccaccaga aagtggtcga
    16861 agaagcacca gcaccgggca ttaccccgga actacgtcgc tacatcggcg aacgttgcgc
    16921 taaagcgtgt gttgatatcg gctatcgcgg tgcaggtact ttcgagttcc tgttcgaaaa
    16981 cggcgagttc tatttcatcg aaatgaacac ccgtattcag gtagaacacc cggttacaga
    17041 aatgatcacc ggcgttgacc tgatcaaaga acagctgcgt atcgctgccg gtcaaccgct
    17101 gtcgatcaag caagaagaag ttcacgttcg cggccatgcg gtggaatgtc gtatcaacgc
    17161 cgaagatccg aacaccttcc tgccaagccc aggcaaaatc acccgtttcc acgcacctgg
    17221 cggttttgga gtgcgttggg agtctcatat ctacgcgggc tacaccgtac cgccgtacta
    17281 tgactcaatg atcggtaagc tgatttgcta cggtgaaaac cgtgacgtgg cgattgctcg
    17341 catgaagaat gcgctgcagg agctgatcat cgacggtatc aaaaccaacg ttgatctgca
    17401 gatccgcatc atgaatgacg agaacttcca gcatggtggc actaacatcc actatctgga
    17461 gaaaaaactc ggtcttcagg aaaaataatt tgtgacgcgc ggtgttttca tcgcgcttag
    17521 caaagaacgt aatcaacgcc gggcctgtta acgctgcccg gcgtttttat tgccgttagc
    17581 taacggtagc ctgcaatttc cgctttcaac gcgctcattt cagcatcgcc atcaacaatt
    17641 cccagctcaa agcagtattt ctttgtttcc cctggctcaa taaagggcag acgcccctgc
    17701 gctcgttctt tatcacgacc gtcaggaaaa ctattactga cctccagtcc cataacatac
    17761 tcacccgcgc ctaaattttt ccactggata aagttgggta gctgcatggt gtcgaatttc
    17821 tcgtaaattc cccaccccag ttcggcgttc accagagcaa ctttactcat cccctgactg
    17881 tcgctattca acgtgagata gtaaacctgc tcgtcaaaac cgggctgtgg ggtacaaatc
    17941 tccagatgac tggttaaccc ttccgccgca tgcggagttc gtggcgttac gcttttagcc
    18001 gacagcaaga tttctggcct gtggcgataa taaaggccag ccgtaattaa agtgataaag
    18061 aatttgcagc ggctggcggg tgaaaccatg attagtcacc ttgtcttcaa tgcgcagcac
    18121 attttcgcca taacggcagc gaatggtcct ttccagcgtc agattttcgc cgaacagtcg
    18181 cgtttcccgt actttgccat tgatgacaaa ttcaatgccg tcgtcggtcc gttctgtgcg
    18241 ataccccacc tcttctgctg gagtcgcagc caaacgcccg tgtaacccca gcgtttctcc
    18301 ttcatcctca caaggcgtgc caatgtagct aagtccgcag gtagtcagta accccgcgta
    18361 aaagctacgt aaaaatccgt tgccctgaga ctcaaaaaaa gcgggggcga caatgccgtt
    18421 gggggtgata aaagaaagcg ccgtgtcctt gtagctggcc catgcaatat ccagcgcgcg
    18481 atcttttacc accgtaaatg ccagtcccgc accagtacgg acatcaaacg cttctgtacc
    18541 ctttgctctg ccactacgta actcatagtg tttaattccg gcaacctgcg agagatcgcc
    18601 aatccacgac caaatttcgt ttttcattgt gctgcttccc gaatggacat gtcatggcgc
    18661 tggatgaacg cttcgatttc cgcaaggcga gacgttccct ccattgggcc acgtctggtt
    18721 accgccagcg caccgcaggc attggcatat tgcagcgctc gatggacgtc aaaccctagc
    18781 tggcggcagg caatccacgc gccaccaaag cagtctcccg caccggtcgg gtcaacttct
    18841 tccaccggat agctctcaac atgaaactgc tcattcgcgg agtagtaact ggcaccttga
    18901 ttgccgcgct tcacaatcac ctctttaaca ccttcttcaa gaaaacctgc gattgcacgc
    18961 tccggcgtcg aatgtggcga caagagcaac acttcgccct cgctgggcat atagatatcc
    19021 gttaactcaa gaacaaagtg gagggcgtca cgcatttctg gaatatcgag catctcttta
    19081 cgaatattag ggtcgaaaga aataacacca ccatttgctt taacgatagt taccgctttt
    19141 tttactgcgt ctaccatatg aaacgaaaac agggaagagc ccataatatg aaaatgggta
    19201 cagtccttca gaatattttc atcaacatgc tgcgccgata atttaccgca agcggcattc
    19261 ttaatattaa aaataaaatc gcgatcgcca gagttatgat aagtaacaaa ggcactcccg
    19321 gtagcttcca gaggtaagac tgaaatcccc ctgatatcca caccgtcagc agccagacga
    19381 tgtatattaa tatcaccaaa gccatcattc cccacacagc taatgatgcc gcacggaacg
    19441 ccaagacgtg tcacctggtc gataaaaata gcgggcgcac cactgggata agggccgttc
    19501 cagatacctg gttgatcaaa tggctggccg attttactcg ccataatttc aaccagaatt
    19561 tctccaattg tgaagacctt actcatatga ttaattcctt gaaatgatta gctgcgtttt
    19621 cttttatttc ttacgtcaaa ataaacggct acaagaatga ctaatccttt aaccaccatc
    19681 tggtaaaaat agggaacatt cattaagttc atgccattat taataacgcc gatagttaat
    19741 gcgccgatca ccgtccccac cacggttcca tagccgccgt tcaggctggt gcctcccagt
    19801 acagccgcag caatagcatc cagttcgtag ctcaacgcgg aattcggtta cccggaatag
    19861 aggcgtgagg cgaggatcat cccgctgata cctgccagca cggctgtaat cataaagact
    19921 ttaattttca agcgggtgac atttatccct gaataaattg ccgcttcttt attaccacca
    19981 gtgatataaa tttttcgtcc aaaggttgtt ttagtaagaa tgaagtggtt aatcagcaat
    20041 aagatgatta atatccagat tggaataggt atatagagaa attctccatt ccccagtgcc
    20101 agaaaggcat catctttaat catgatgggc ataccatcgg tcactatata agcgatacca
    20161 cggaagatcc ccatagtagc aacggtgaca ataaacgacg ggatccccgc tatagccgtc
    20221 aagccaccat ttatcatccc cagagccagt gctgcaataa tggataatgg gaccaataaa
    20281 ataaatggaa ttccccatga agccccaagc gcagcaaatg tgccaatcaa tgcaatggtt
    20341 gaaccgaccg ataaatcaat atcgccaagc aaaagtacat aagtcattcc ataggcggta
    20401 attgcaatta tcgaaacctg aagaataata ttagtaatgt tgttggcggt aaaaaaactg
    20461 tcattaataa aactgaacag aatgattaac accaccattc cactgaggat accgccatat
    20521 tgcctgaaga cttttttaaa attatttgca atgggaattt ccgaaggttt cggactggca
    20581 agagttttct ctgtcattta acgtactcct gttgcagcgg tcattacctg tgtctgcgtt
    20641 aaattttcac atgaataaat atcggcaatc gttttattac gcattaccac cagacgatcg
    20701 ctaagtgcca tgacttctgg cagttcagag gacaccagaa taattgctgt ccctgccgca
    20761 gccagttgac gtatcacgtt atagatttca aattttgcgc cgacatccac tccgcgcgtt
    20821 ggctcatcga gcaataaaat acgcggagtt ttctccagcc acttcgataa cacgattttc
    20881 tgttgattac cgccgctcag cgctcccact gcctgatgta cagaactcac tttagtgttc
    20941 attctggcaa ttgattgcag cgtcttcgcc tcttcttgtc ggtgattgat gagtcccatc
    21001 cgcgaagcgt tatattgaaa cgccacatgg tgcgtgttca tattgacgtc gtgcatcagc
    21061 actaaccctt cttccttgcg gttttcggtc acaaaagcaa tgccatgatc aatagcctgt
    21121 ttaggattag tgatcctgac ttccttacca tcaatcaaca cagtcccctg atacgaaacg
    21181 aggccaaaga gcgccttcat cacatcggaa cgcccggccc ccaccaggcc aaaaaatccg
    21241 aggacctccc ccggacgcac agcgaaactg atctctttaa aaagcgaagg atgggaaaga
    21301 ttttttactt ccagttttgc aggaacattt tcatccggta tttccccaag acgtgctggc
    21361 caaatatttt tcatttcccg accaaccatt tgggcaatca aatgctggat atcactgttt
    21421 ttcttttcat gcgtggcgat atactcaccg tcgcggatca ccgaaatatc atctgaaatc
    21481 cgcataattt catccatgcg atgagagata taaataaccg cacagcctcg ctgctttaat
    21541 ttttcaataa tgttaaacag aatctccgct tcattatcac ttagcgaaga tgtcggttca
    21601 tccataatca cgacatccgc tgcatagctt aaggctttag caatttctac cagttgccta
    21661 tgtgccatcg acatattgcc gaccatttct agtgggtcta gtggtatgcc ccgttccgcg
    21721 aggagattct ggcaatctgc gagcattttt ttatcgttaa taatgccaaa acgtcgcggt
    21781 tcattattgg cataaatatt ttccgccacg gtcatattat tactcaggct caattcctga
    21841 aaaataatcg caattcctaa ctggcgtgaa tgctgcggat tattaatggt gatagtttca
    21901 ccacgtagtg ttatcgttcc ctgggttggc ggataaacac cggccagtat tttcattaat
    21961 gtcgatttac cggcaccatt ctcgcctaat aaagtatgca cccgcccttt acgtaaagag
    22021 agggtaatat cttttaatgc ttgaacatga ccaaatgtct tcgcaatatg cgaaacatca
    22081 agaataacat ccgtcataat gacctcggtg tcacaacaaa acgccaataa cgttttgttg
    22141 tgaggtgaag atgtttacta tttacctgta taaacgccgg gttgtaccgg gatcattttt
    22201 tctaccggtt taccattgag gtgatcaaca gcggcatcaa tggctttagc acccatttga
    22261 tcgggatatt gccgaatcac ggccgcaaac gttttttcac tgtcaacggc atcacgcgct
    22321 tctttcatgc catcaaaacc aatgattttg atgttgtcgt tatgcgcaga ttttgctgcg
    22381 ataaccgctg ccagcgccgc atcatcacca aagccaaaca gaccattcag gttggcattt
    22441 gcctgcataa tgttttgcgc agtcgttaac gcttcagccc gcgtaatact aggctgttca
    22501 gcaacaattt taatatccgg tgtatcgctt aaccctttct taaaaccatc aacgcgcgca
    22561 accaccgatt gtagtgccgg ataagaaata atggctacag tacctttacc attcaaaagt
    22621 ttgcccatta acttgcctgc ttcaacgcct cccgcatagt tatcggtcgc aacatgcgat
    22681 acgaccggaa cgccttccgc agcgacgtcg acggtgataa cgggaatacc ggccttatcg
    22741 gctttaatta tcgccgcctg gacgcctttc gaatcgaccg gagagataat aatggcatcc
    22801 acttttttag taataaaatc ctcaacatca gaaagttgtt tgtttaaatc ctgattggca
    22861 aatag
//
LOCUS       DAJTMC010000056        22617 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.056, whole genome shotgun sequence.
ACCESSION   DAJTMC010000056 DAJTMC010000000
VERSION     DAJTMC010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22617
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.056"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(50..1699)
                     /gene="actP"
                     /locus_tag="OO130_001899"
     CDS             complement(50..1699)
                     /gene="actP"
                     /locus_tag="OO130_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HCR5365334.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGMLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGTMLGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(1696..2010)
                     /locus_tag="OO130_001900"
     CDS             complement(1696..2010)
                     /locus_tag="OO130_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HCR5365335.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            complement(2142..4101)
                     /gene="acs"
                     /locus_tag="OO130_001901"
                     /pseudo
     CDS             complement(2142..4101)
                     /gene="acs"
                     /locus_tag="OO130_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
     gene            4494..5930
                     /gene="nrfA"
                     /locus_tag="OO130_001902"
     CDS             4494..5930
                     /gene="nrfA"
                     /locus_tag="OO130_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HCR5365336.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            5975..6541
                     /gene="nrfB"
                     /locus_tag="OO130_001903"
     CDS             5975..6541
                     /gene="nrfB"
                     /locus_tag="OO130_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HCR5365337.1"
                     /translation="MSVLRSLLTARVLASGLLWSLNGITATPAAQASGDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            6538..7209
                     /gene="nrfC"
                     /locus_tag="OO130_001904"
     CDS             6538..7209
                     /gene="nrfC"
                     /locus_tag="OO130_001904"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HCR5365338.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            7206..8162
                     /gene="nrfD"
                     /locus_tag="OO130_001905"
     CDS             7206..8162
                     /gene="nrfD"
                     /locus_tag="OO130_001905"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HCR5365339.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLAQQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKSWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            8182..9900
                     /locus_tag="OO130_001906"
     CDS             8182..9900
                     /locus_tag="OO130_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HCR5365340.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQCALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDAASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELMPERRFYEARRQ
                     QMMEPSIRWNGIHDWYVVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GRKRDE"
     gene            9893..10276
                     /gene="nrfF"
                     /locus_tag="OO130_001907"
     CDS             9893..10276
                     /gene="nrfF"
                     /locus_tag="OO130_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HCR5365341.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVAEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            10273..10869
                     /gene="nrfG"
                     /locus_tag="OO130_001908"
     CDS             10273..10869
                     /gene="nrfG"
                     /locus_tag="OO130_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HCR5365342.1"
                     /translation="MKEPKIPVKMLTTLTILMVFLCVGSYLLSPKWQAVHAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYVALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            11210..12523
                     /gene="gltP"
                     /locus_tag="OO130_001909"
     CDS             11210..12523
                     /gene="gltP"
                     /locus_tag="OO130_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HCR5365343.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(12714..13402)
                     /locus_tag="OO130_001910"
                     /pseudo
     CDS             complement(12714..13402)
                     /locus_tag="OO130_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
     gene            complement(13496..15643)
                     /gene="fdhF"
                     /locus_tag="OO130_001911"
     CDS             complement(13496..15643)
                     /gene="fdhF"
                     /locus_tag="OO130_001911"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(15224..15226),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HCR5365344.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIMANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGEQGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            complement(15841..17307)
                     /gene="mdtP"
                     /locus_tag="OO130_001912"
     CDS             complement(15841..17307)
                     /gene="mdtP"
                     /locus_tag="OO130_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HCR5365345.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPSLGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGIAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDSIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIHSIQLMKSLGGGYQAAPVVEKK"
     gene            complement(17304..19355)
                     /gene="mdtO"
                     /locus_tag="OO130_001913"
     CDS             complement(17304..19355)
                     /gene="mdtO"
                     /locus_tag="OO130_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HCR5365346.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAISQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAVAEGQCWQSDWRISESEAVAARECNLENICQTLLQLGQMNPNTPPTPA
                     AKPPSMVADAFTNPDYIRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLD
                     SEERALLIERSQTVIRQGRDLLHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGL
                     ATALSCSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGAT
                     Q"
     gene            complement(19355..20386)
                     /gene="mdtN"
                     /locus_tag="OO130_001914"
     CDS             complement(19355..20386)
                     /gene="mdtN"
                     /locus_tag="OO130_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HCR5365347.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDVLVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPNDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            complement(20405..20680)
                     /locus_tag="OO130_001915"
     CDS             complement(20405..20680)
                     /locus_tag="OO130_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5365348.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILTLITRRIIQRANINLAFVGIIYTALFAVYAMLFWLAFF"
     gene            complement(20889..>22617)
                     /gene="yjcS"
                     /locus_tag="OO130_001916"
     CDS             complement(20889..>22617)
                     /gene="yjcS"
                     /locus_tag="OO130_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HCR5365349.1"
                     /translation="GEDYKFDINAPAPETVNPSLWRQSQLNGISGLFKVTDRMYQVRG
                     QDISNITFIEGDTGIIVIDPLVTPPSAKAALDLYFQNRPQKPIVAVIYTHSHTDHYGG
                     VKGIISEADVKSGKVQVIAPAGFMDEAISENVLAGNIMSHRALYSYGLLLAHNPQGNI
                     GNGLGVTLASGYPSIIAPNKTITKTGEKMIIDGLEFDFLMTPGSEAPAEMHFYIPALK
                     ALCTAENATHTLHNFYTLRGAKTRDTSKWTEYLNETLDMWGNDAEVLFMPHTWPVRGN
                     KHINDYIGKYRDTIKYIHDQTLHLANQGYTMNEIGDMIKLPPALANNWASRGYYGSVS
                     HNARAVYNFYLGYYDGNPANLHPYGQVEMGKRYVQALGGSARVISLAQEANKQGDYRW
                     SAELLKQVIAANPGDQVAKNLQANNFEQLGYQAESATWRGFYLTGAKELREGVHKFSH
                     GTTGSPDTIRGMSVEMLFDFMSVRLDSAKAAGKNISLNFNMSNGDNLNLTLNDSVLNY
                     RKTLQPQADASFYISREDLHAVLTGQAKMADLVKAKKAKIIGNGAKLEEIIACLDNFD
                     LWVNIVTPN"
BASE COUNT         5074 a         5960 c         6194 g         5389 t
ORIGIN      
        1 ctggagagga agagtattcc ccggtcaggc gaccggggaa ggggagagat taatgtgcgc
       61 ggccttgctc aacgccaaag ccggtctggg agcggataaa ctgcgcacgg aacagttcac
      121 gctcgcgcgc accttccgct gagttatcgg ttgccgagaa gaaccagatg ccgaggaatg
      181 ccacgctgat agagaacagc gccgggtatt catacgggaa gatggctttt tcgtgaccga
      241 ggatctgtac ccaaatagtc gggccgagga tcatcagcac cactgcggta atcaacccca
      301 gccagccgcc cagcatcgtg ccacgggtag tcagtttcga ccagtacatg gaaagcagaa
      361 tgatcgggaa gttacagctc gccgcgatgg caaacgcaag gcctaccata aaggcgatgt
      421 tctgattctc aaacagcatg ccgaggataa tcgcaatcac acccaaaatc aggacggtga
      481 ttttcgatac ccgcagttct tcacgttcgg tcgcaccttt tttgaagacg ttagcgtaca
      541 agtcatgcga aaccgcggat gcgcccgcca gcgtcagacc cgcaaccacc gcgaggatag
      601 tggcgaaagc aaccgctgaa ataaaaccga ggaacaggtt gccgcccact gcattcgcca
      661 ggtgaacggc cgccatgttg ttaccaccaa tcagatggcc cgccgcgtct ttatattccg
      721 gattcgcacc aaccagcatg atcgcgccga agccgataat aaaggtcaga atatagaagt
      781 agcccataaa cccggtggcg tagaacacgc tcttacgtgc ttcgcgggca tcgctgactg
      841 taaagaagcg catcagaatg tgcggcaagc ccgccgtacc aaacatcagt cccagaccca
      901 gagagagcgc ggagatcgga tctttcacca gcccgcccgg cttcatgatg tcgacacctt
      961 tcgggtgtac cgccatcgct tcgctgaaca gattattgaa gctaaagccg acgtgtttca
     1021 tcaccataaa ggccataaag ctggcaccga acagcaacag cacggcttta ataatttgca
     1081 cccaggtggt cgccagcatg ccgccgaaca ggacgtacat catcatcagc acgccgacca
     1141 gcaccaccgc aatgtgatag ttaaggccaa acagcagctc gatcagttta cctgcgccta
     1201 ccatctgggc gataaggtaa agcgccacca ccaccagaga accacaggcc gaaagaatac
     1261 gaatcggccc ttgtttcagg cggtaagagg ctacatcggc aaaggtgtag cgtcccaggt
     1321 tacgcagacg ttcggcaatc aggaacaaaa tgatcggcca gcccaccagg aagcccagcg
     1381 agtaaattaa gccgtcatag ccggaggtaa acaccagcgc ggagatcccc aaaaatgagg
     1441 cggcggacat atagtcaccg gcaatcgcca gcccgttctg gaagcctgtg atattgccgc
     1501 ctgccgtgta gtagtcgcta cgagaacgta cgcgttttga tgcccagtag gtaatgccga
     1561 gcgtaaacac gacgaaaatc aggaacataa taatcgcctg ccagttcgtt ggctggcgct
     1621 ctacggcccc gctaatagca tccgcggcgt tagctgcgaa agggagtgtg gcggcaagcg
     1681 ccgtcagaac tcttttcatg atgcttgtac ctcatgcagg acttcattat taagacggtc
     1741 gaattcgccg ttcgcccgcc agatgtagat accggtgaga acaaaggaga tcacaatcac
     1801 tccaacacca attggaatac cgcgtgtgac gctggtgttc ggattcagcg gcgtgcccag
     1861 ccagccgggc gcgaaggcga tcagtaaaat aaagccgata taaactgcca gcataataat
     1921 cgacaggatg gtggcaaacc gttgccgttt ttcgactaac tccctgaaat gcgcattgtc
     1981 ttctatccgc tgataaatag tgccattcat cacagattct ccagaggtaa tgtagggatt
     2041 gttttaattc ccgctccctt atgggagaag gttaacgctc gggtaaccct tgccgaatgt
     2101 aggccggata aggcgtttac gccgcatccg gcaattgtgg gttacgatgg catcgcgata
     2161 gcctgcttct cttcaagcag cttctcgact acgccaggat cggcaagcgt cgaggtatcg
     2221 cccaggttgc tggtatcgcc cgccgcaatt ttgcgcagaa tacggcgcat aattttgccg
     2281 gagcgggttt taggcaggga gtcggtccag tgcagcacgt ctggcgtcgc cagcgggcca
     2341 atctctttac gcacccagtt gcggacttct gcgtacagtt ctggtgacgg ttcctccccg
     2401 tgattaagcg tgacgtaggc gtagatcgcc tgacctttaa tattgtgcgg aatacctact
     2461 acggcggctt cggcaatctt cggatgcgcc accagcgccg actcaatctc tgccgtcccc
     2521 agacggtgac cggagacgtt cagcacgtcg tccacatgcc cggtgatcca gtaatagcca
     2581 tcttcatcgc gacgcgcgcc gtcgccgctg aaatacatat ttttgaaggt ggagaagtag
     2641 gtctgttcaa aacgttcgtg atcgccaaac agcgtacgcg cctgacccgg ccaggagtcg
     2701 gtgatcacca ggctgccttc ggtagcgcct tccagcgggt taccttcgtt atcgaccagc
     2761 gccggttgca cgccgaagaa cggacgtgtt gccgaaccgg ctttcagccc ggtagcgcca
     2821 ggcagtggcg tgatcatgaa accgccagtt tcggtctgcc accaggtatc gaccaccgga
     2881 catttctcgt tgccgatttt tttccagtac cactcccacg cttccgggtt aattggctcg
     2941 cccacggaac cgagaatgcg cagcgacgaa cggtcggtac cctcgatcgc tttatcgcct
     3001 tccgccatca gcgcgcggat cgccgtgggt gcggtataga gaatattgac ctgatgcttg
     3061 tccaccacct gcgccatacg ggcaggcgtc ggccagttgg gtacgccttc aaacatcagc
     3121 gtggtcgcac cgcaggccag cgggccgtac agcaagtaac tgtgtccggt cacccagccc
     3181 acatcggcgg tgcaccagta gatatcaccc ggatgataat caaagacata tttaaaggtc
     3241 agcgccgcgt acaccagata accgccggta gtatgcagca caccttttgg cttaccggta
     3301 gaaccggagg tgtagagaat aaacagcgga tcttcggcgt tcatctcttc cgcctggtgc
     3361 tgatcgctcg cttgctcaac caggtcgtgc caccacaggt cgcgcccttc ctgccagtca
     3421 attttcccgc cagtacgctt cagtaccacc acatgctcta cgctggtgac gttcgggttt
     3481 ttcagcgcgt catcaacgtt tttcttcagc ggaatactgc cgcccggcac gcacaccttc
     3541 gtcggaagtg atcaccagtc gtgagttgga atcaataatg cgcccggcaa cggcttccgg
     3601 cgagaagccg ccgaaaatca ccgaatgcac cgcgccaatg cgggcgcagg ccagcatcgc
     3661 aaccgcggct tccggcacca tcggcatata aatcgccacc acatcacctt ttttaatgcc
     3721 cagctcgagc agggtattgg cgaagcggca gacgtcgcgg tgcagctctt tatagctgat
     3781 atgtttgctc tggctggcgt cgtcgccttc ccagatgatg gcggtacgat cgccgttttc
     3841 ttgcagatgg cggtcaaggc agtttgccgc cagattcagc gtgccgtcct cgtaccattt
     3901 aatggacaca ttaccggggg caaaggaggt gtttttcacc ttctggtaag gtttgatcca
     3961 gtcaagaatt tttccctgtt cgccccagaa ggtatcaggt acgttaatag attgttgata
     4021 cattgcctcg tactgctgag ggtttatcag gcaacggtct gcgatgttgg caggaatggt
     4081 gtgtttgtga atttggctca tgcttttgtt ctccttgtag aatgttaata atatgtgaca
     4141 taagcgttaa atgtaggggt attggcagtt ttgtttagta tttgggcgac agatcacgca
     4201 aaagtagaat tgtgcaaata aacggcaggg taatttttga aggtcagcaa caaaagttga
     4261 ttaattcttt gaggaacatg cagttatgca tgctgttgaa aaagaggaag atactgacta
     4321 actctaaagt ggtattttac atgcacttac aattgattaa agacaacatt cacagtgtgg
     4381 ttatttgtta cacatagggg cgagcaatgt catgacagtg taggtgcggt tactgtcgtg
     4441 aaaaacaata aaaaccgcca ttgcaacaat ggcgcaattc ggatgaagcc cctatgacaa
     4501 ggataaaaat aaacgcacgc cgtatcttca gcttattgat tccttttttc tttttcactt
     4561 ctgttcacgc tgaacaaacg gcagctcccg caaaacctgt aactgtggaa gcgaagaatg
     4621 aaacctttgc cccgcagcat cccgatcaat atctctcctg gaaagccacc tcggaacagt
     4681 cagagcgtgt tgacgccctg gcggaagatc cacggctggt gatcctgtgg gcggggtatc
     4741 ccttctcgcg cgattacaac aagccgcgtg gacatgcctt tgctgtgacc gatgtgcgtg
     4801 aaaccctgcg taccggtgcg ccgaaaaatg ctgaagatgg tccgctaccg atggcgtgct
     4861 ggagttgtaa aagcccggat gtggcgcgtc tgatccagaa agacggcgaa gatggctact
     4921 tccacggtaa gtgggcgcgc ggcggcccgg aaatcgtcaa caacttaggt tgtgccgact
     4981 gccataacac cgcctcacca gagttcgcca aaggcaaacc ggagttaacc ctttcccgtc
     5041 cgtatgcggc tcgcgcgatg gaagccattg gtaaaccttt tgagaaagcc ggacgtttcg
     5101 accagcaatc gatggtttgc ggtcagtgcc atgtggagta ttacttcgac ggcaaaaaca
     5161 aagcggttaa attcccgtgg gatgacggca tgaaagtcga aaatatggag cagtattacg
     5221 acaaaattgc cttctctgac tggactaact ccctgtcgaa aacgccaatg ctgaaagcgc
     5281 agcacccgga atatgaaacc tggacagcgg gcattcacgg taaaaacaac gtgacctgta
     5341 tcgactgcca tatgccaaaa gtgcagaacg ccgaaggcaa actctacacc gaccataaaa
     5401 ttggtaatcc gtttgataac ttcgcccaga cttgtgcgaa ctgccatacc caggacaaag
     5461 ctgccttgca aaaagtggtc gcggaacgta agcagtcgat taacgacctg aaaatcaagg
     5521 ttgaagatca actggttcac gctcacttcg aagcgaaagc agcgctggat gcaggcgcga
     5581 cggaagctga aatgaagcca attcaggacg atatccgtca tgcccagtgg cgctgggatc
     5641 tggcgatcgc ttcccacggc attcatatgc acgcaccgga agaaggttta cggatgctcg
     5701 gtacggcgat ggataaagcg gcggatgcac gcaccaaact ggcgcgcctg ctggcgacca
     5761 aaggcatcac ccatgaaatc cagatcccgg atatctcaac caaagagaaa gcccagcagg
     5821 ccattggcct gaacatggaa caaatcaagg ccgagaagca ggacttcatc aaaacggtga
     5881 tcccgcagtg ggaagaacag gcacgtaaaa acggtctgtt aagccaataa ccccgttccg
     5941 cctcgcaagg ggcggaaaac acaatggagt gaatatgagc gtattacgtt cgttattaac
     6001 tgctagggtg ctggcgtcgg gcctgttgtg gagcctgaac ggaattaccg ctacccctgc
     6061 ggcgcaggca tctggtgatc gctacgaagt tacccagcag cgtaacccgg atgccgcctg
     6121 tctggactgt cataaaccag ataccgaagg tatgcatggc aaacatgctt ccgttatcaa
     6181 cccgaataac aaattgccgg tcacctgcac caactgtcac ggccagccat caccgcaaca
     6241 ccgcgaaggg gtgaaagatg tgatgcgctt taacgagccg atgtacaagg tcggagagca
     6301 gaacagcgtc tgtatgtcct gtcatctgcc ggaacagttg caaaaagcgt tctggccgca
     6361 cgatgtccac gtaaccaaag tggcgtgcgc cagctgccat tccctgcatc cacagcaaga
     6421 tacgatgcag acgttaagtg acaaaggacg gattaagatt tgcgtcgatt gccacagcga
     6481 tcagcgcacc aatccgaact ttaacccggc gtctgttccg ttgcttaagg agcagccatg
     6541 acctggtctc gtcgccagtt tctcaccggc gtcggcgtgc tggcagccgt cagcggcacc
     6601 gcagggcgcg tggtggcgaa aacgttgaat atcaatgggg tgcgttacgg catggtgcat
     6661 gacgagtcgt tatgcatcgg ctgtaccgcc tgtatggatg cttgtcgaga agtgaacaag
     6721 gtgccggaag gcgtctcgcg cctgacgatt attcgtagcg agccgcaggg cgaatttcct
     6781 gatgtgaaat atcgcttctt ccgtaagtct tgccagcact gcgatcatgc gccgtgcgtt
     6841 gacgtctgcc cgaccggtgc gtcttttcgt gatgccgcta gcgggattgt cgatgttaat
     6901 cctgatctct gcgtcggttg tcagtactgc atcgccgcct gtccgtaccg cgtgcgcttt
     6961 atccatccgg tcacgaagac ggcggacaaa tgcgatttct gccgtaaaac caatttgcag
     7021 gccggtaagc tgcccgcgtg cgttgaggct tgcccgacga aggcgctgac gtttggcaat
     7081 ctggacgatc ctaacagtga gatttcgcaa ctgctgcgcc agaagcctac ttaccgctac
     7141 aagctggcgc tgggaaccaa accgaagctg taccgcgtac cgtttaaata cggggaggtg
     7201 agccaatgac gcagacttcc gcatttcatt ttgaatcgct ggtgtgggac tggccgattg
     7261 ccatctacct gtttttgatt ggtatttctg ccggtctggt gacgctggcc gtgctgttac
     7321 gtcgcttcta cccgcaggcg ggcggtgcag acagtacgtt gctgcgcacc acgctgattg
     7381 tcgggccggg cgcggtgatc ctcggtctgt tgatcctcgt cttccacctg acaagaccgt
     7441 ggaccttctg gaagctgatg ttccactaca gttttacctc ggtgatgtcg atgggggtga
     7501 tgctgtttca gctttacatg gtggtgctgg tgctgtggct ggcgaaaatc tttgaacatg
     7561 atttgcttgc ccagcaacaa cgctggttgc cgaagctggg gatcgtgcaa aaggttctga
     7621 gcctgctgac gcccgttcat cgcggactgg aaacgctgat gctggtgttg gcggtgctgc
     7681 tgggggctta taccggcttt ctgctgtcgg cgctgaaatc gtatccgttc ctcaataacc
     7741 cgatcctgcc ggtgctgttc ctcttctccg gcatctcgtc cggtgcggcg gtggcgctga
     7801 tcgccatggc gatacgccaa cgcagtaacc cgcattccac ggaagcgcac tttgttcacc
     7861 ggatggaaat ccccgtggta tggggtgaaa tcttcctgct ggtggcgttt tttgtcggtc
     7921 tggcgctggg cgatgacggt aaagttcgtg cgctggtggc ggcattaggt ggcggtttct
     7981 ggacgtggtg gttctggctt ggtgtcgccg ggctggggct gattgtgcca atgttgctca
     8041 aatcgtgggt caatcgcagt tccggcattc ctgccgtgct ggcggcgtgt ggggccagtc
     8101 tggtcggcgt gttgatgctg cgctttttca ttctctacgc cggacagttg acggtggcgt
     8161 aagccagaaa agaggtggtt tctggacgta ttccttcctg aagtcggttt tctggcgttg
     8221 ttgttaagtc tcggggtcaa cgtgttgacc ccgttgacgg cctttgcggg agtgcggttg
     8281 cgctggcctg ccatgatgcg actcacttgc atcggcattc tggcgcagtg cgcgctcctg
     8341 ctgctcgcct ttggcgtact gacgtattgt tttctcatca gcgatttctc ggtcatttat
     8401 gtcgcccaac atagctacag cctgctgtcg tgggaactca aactggcggc ggtgtggggc
     8461 ggtcatgaag gttcgctgct gctttgggtg ctgctgcttt ccgcctggag cgcgctgttt
     8521 gcctggcatt atcggcagca aaccgatccg ctatttccgc tgacgctagc cgttttatct
     8581 ctcatgctcg ccgcactgct actgtttgtg gtgctgtggt ccgatccctt cgtgcggata
     8641 tttccaccag caatcgaagg ccgcgatctc aatccgatgc tgcaacatcc cggtcttatc
     8701 tttcatccac cgctgcttta ccttggctat ggcggtttga tggtagcggc gagcgtggcg
     8761 ctggcgagtc tactgcgcgg cgagtttgat gcggccagcg cccgaatttg ctggcgctgg
     8821 gcgttacctg gctggagtgc attaacggcg gggatcatcc tcggttcctg gtgggcctac
     8881 tgcgaactcg gctggggcgg ctggtggttc tgggatccgg tggaaaatgc ctctttatta
     8941 ccctggcttt ctgccactgc gctgctgcac agtttatccc tgacacgcca gcgggggatt
     9001 tttcgccact ggtcgctgtt actggcgata gttactctga tgctgtcgct gctgggcacc
     9061 ttaattgtcc gttctggcat tctggtttcg gttcatgcgt tcgcgctgga taacgtccgc
     9121 gccgtgccgt tgttcagcct gtttgcactg attagccttg cgtctctggc tctgtatggc
     9181 tggcgagcgc gggacggtgg cccggcggtg cgtttttcgg ggttatcgcg ggaaatgtta
     9241 atcctcgcta cgctgttgct gttttgcgca gtgctactga tcgtgctggt gggaacgctt
     9301 tatccgatga tttacggtct gctgggctgg ggacgcctct ccgttggcgc gccgtatttt
     9361 aaccgcgcga cgttaccgtt tggtctgttg atgctggtgg tgattgtgct ggcgacgttt
     9421 gtctctggca aacgcgtgca gcttccggcg ctggtagctc atgcgggcgt gctgttattt
     9481 gccgctggga tcgtggtttc cagcgtcagc cgtcaggaga tcagcctgaa tttacagccg
     9541 ggtcagcagg tgacgctggc aggatacacc ttccgttttg agcgcctcga tctgcaagcc
     9601 aaaggcaatt acaccagcga aaaagcgata gtggcactgt ttgaccatca gcaacgcatt
     9661 ggtgaactga tgccggagcg gcgtttttac gaagcacgtc gtcagcaaat gatggaaccg
     9721 tcaattcgct ggaacggcat ccatgactgg tatgtggtca tgggtgaaaa aaccggagcg
     9781 gatcgttacg cttttcgctt gtatgtacaa agcggtgtgc gctggatctg ggggggagga
     9841 ttgttgatga ttgcgggcgc attgttaagc ggatggcggg ggaggaagcg cgatgaataa
     9901 aggacttctc acgctactgc tgttatttac ctgttttgcg cgcgctcagg tcgtagacac
     9961 ctggcaattc gccaatccgc aacaacagca acaggcgtta aatattgcca gccagttacg
    10021 ttgtccgcag tgccagaatc aaaacttact ggaatccaac gcgccggtgg cagtcagtat
    10081 gcgccatcag gtttacagca tggtggcgga ggggaagaac gaagtcgaaa tcattggctg
    10141 gatgaccgaa cgctacggag attttgttcg ctataaccca ccgttaacgg gtcagacgct
    10201 ggtgttgtgg gcgctgccag tggtgttgtt actgctgatg gcactgatcc tctggcgagt
    10261 gagggcgaag cgatgaaaga gcccaaaata ccggtgaaaa tgctgacaac actcacgatt
    10321 ttgatggtat ttctctgtgt cggcagttat ctgttaagtc caaaatggca ggcggtacat
    10381 gcggaatatc agcgtcagcg cgatccgcta catcagtttg ccagccagca aaccccggaa
    10441 gcgcagcttc aggcattgca ggataaaatc cgcgctaatc cacaaaacag cgaacaatgg
    10501 gcgttactgg gcgagtacta tctgtggcaa aacgattaca gcaattcgct gctggcgtac
    10561 cgtcaggcgt tgcaactacg tggtgagaac gctgaactgt atgtggcgct ggcgacggtg
    10621 ctttattacc aggccagcca gcatatgacc gcccagactc gcgcaatgat cgacaaagcc
    10681 ctcgcgctgg actctaacga aatcaccgct ctgatgctgc tggcttccga tgcgtttatg
    10741 caggcgaatt acgcgcaagc catcgaacta tggcaaaaag tgatggatct caactcaccc
    10801 cggatcaacc ggacacagct ggtcgagtcg attaatatgg cgaaattgtt gcagcggaga
    10861 tcggattaat catctggtga tgaagattat gttattgagc tgcataaaaa ataatcgaat
    10921 gaacatatgc caaaaataat cactaatcag tattattgca gattaaacaa ataaaaatct
    10981 ttccataaca aatggttatt cattaatcct gacataaact cgcgttatta tgcattaatg
    11041 cagcgaaaag ctctgttgtt aaagggttgc gcaacatacc gcgcaaatga tactgatcat
    11101 aagcgttaaa aaaatctaca aaccaacgca acacaattca tgcctggcag tatgtcacgt
    11161 tctcgcgttt ctgaacgggg aacggcgctc cattgaggaa gttattctga tgaaaaatat
    11221 aaaattcagc ctggcctggc agattctgtt tgctatggtg ctgggcattc tcctgggaag
    11281 ctacctgcac taccatagcg acagccgcga ctggctggtc gtcaatttgc tctctccggc
    11341 gggtgatatc ttcatccatc tgattaaaat gattgttgtg ccgattgtga tctccacgct
    11401 ggtggtgggt atcgcgggtg tgggtgatgc caaacagctc gggcgtattg gcgcgaaaac
    11461 cattatttac ttcgaggtga tcaccaccgt cgccatcatt ttggggatca ctctggcgaa
    11521 cgtcttccag cccggtgccg gggtggatat gtcgcagctg gcgaccgtcg atatctcgaa
    11581 atatcagagc actacggaag cggtacaaag cagttcccac ggcattatgg gcacgatttt
    11641 gtcgctggtg ccgacgaaca ttgtggcgtc gatggcgaaa ggcgaaatgc tgccgatcat
    11701 cttcttctcg gtgctgtttg gtctggggct ttcctccctg cccgcgacgc atcgtgaacc
    11761 gctggtgacc gtgttccgct ccatctctga aaccatgttt aaagtgactc acatggtgat
    11821 gcgttatgca ccggtgggtg tgtttgcgct gattgcggtg acggtggcta actttggttt
    11881 ctcttctctg tggccactgg cgaaactggt gctgctggtg catttcgcca ttctgttctt
    11941 cgcgctggta gtgctgggaa ttgtggcgcg cctgtgcggg ttaagcgtct ggatcctgat
    12001 tcgtattctg aaagatgagc tgattctggc gtactccact gccagctctg aaagcgtact
    12061 gccgcgaatt attgagaaga tggaagccta cggagcaccg gcgtcgatca ccagcttcgt
    12121 ggtgccgacc ggttactctt ttaaccttga tggttcgacg ctgtatcaaa gtattgccgc
    12181 tattttcatc gctcagctgt atggcattga cctgtccatc tggcaggaaa tcattctggt
    12241 gctgacgctg atggtgacct cgaaggggat tgctggcgtg ccgggcgtgt cgtttgtggt
    12301 gttgctggca acgctgggta gcgtaggtat tccgctggaa ggtctggcgt ttattgctgg
    12361 tgttgaccgt atcctcgaca tggcgcgtac tgcgctgaac gtggtgggta atgcgctggc
    12421 ggtgctggtg attgccaagt gggaacacaa atttgaccgc aaaaaggcgc tggcttatga
    12481 gcgtgaagtg ctgggcaaat ttgataaaac tgcggatcaa taattgaaga ttgccggggt
    12541 tatccacccg gcaatgtgtg gatgcctgat gcgacgctgg tgcgtcttat caggcctacg
    12601 ccagacagcg caatagcctg atttagcgtg attttgtagg tcggataagg cgtttacgcc
    12661 gcatccgaca tcaacgcctg atgcgacgct taacgcgtct tatcaggcct accttaaccg
    12721 ttggttaatt tttcaaactc ttcacacccg gtatcaaacc cttccataca gctcaggttc
    12781 aaccagtgca gcgccttttg tttgttcttc tcgataaaac cttcttcacc gtttaagaac
    12841 atcattcccg cccagtactc ggaataaccg gttcgggaaa ttgcagagct gcgtttgaaa
    12901 tactaggttg ccttgtcatc gtcggcctta atacccacgc cgttggcgta aatcaaacca
    12961 agcagcattt gggcatcgac ggcagagtcg ttctccagat cttccgaggc ttttctaaca
    13021 gcgaaatcgc ctttggataa tccggtttac ccgcttgagt atttaccaga atatgcgcca
    13081 gcgttacttc accttcttta ctgcccgctt gcgccgcttt ctctgcaaga acttttgctt
    13141 gtggatagtc cagactgccc ggattggtga ttttgatctg cgccaacagc gcgcaggcat
    13201 cagcatcacc gctgtcggcg gctttctgtg cccaatattc ggctttgctc aaatcgccgg
    13261 agctaaacca gctgtcggca agaaaatatt gtgcgcgtcg gtccccggcc tctgctgcct
    13321 ttaaatactg gctgccaggc tcggagtcgt tggcgtgggc aaagaatgtc aaaaacaaca
    13381 tcaatgcgat aatttttttc attttgataa tacgattggg tagggagtaa ccagtataaa
    13441 gagttcgaat ggataaaaaa acagcctccg aaaggaggct gtagaaagga ctgtattacg
    13501 ccagtgccgc ttcgcgcagg cgagttttca acttgttgta ctcgtcaatc acgtactgct
    13561 cggcggcgcg ctgatcggcg atcggctcga cgcgcacggc gcagtatttg tattccggcg
    13621 ttttcgtaat cgggcttaag ttttcggtaa ccagctcgtt acaggcacca atccaccact
    13681 ggtaggtcat gtaaatcgcc cctttgttcg gacgatcgct gacctgcgca cgggtgataa
    13741 ttttgccttt acgcgagtgc acccaaacca atgcctcatc ttcaataccc agacgtttgg
    13801 cgtcttcggt attgatttgt gcgtagccag gttcatcagc cagcgccgcc agtgccgcac
    13861 agttaccggt catcgaacgg caagagtagt gaccaacttc acgcaccgtt gacagtacca
    13921 tcgggtactc gtcggtgagt ttgtcgattg gcgctaccca gtcgcaggtg aagaactgcg
    13981 ccagaccgtt cggggtatca aacttctctt taaacagata agaagtcccc tgatcggcat
    14041 ctgaagtatc gcggcaaggc cactgaatga agccctgttc gcccattttc tcgtaagtcg
    14101 caccgtagaa atccgggcac agatgacgca actcatccca gatctcctgg gtgttgttgt
    14161 agtgcatcgg ataacccata cgggtggcga tttcactgat gatttgccag tccgttttca
    14221 gatcccattt cggttcaact gctttgaaga aacgctggaa gccacggtca gccgcagtaa
    14281 acacgccttc atgctcgccc cacgacgttg acggcaaaat gacatccgcc gccgacgcgg
    14341 ttttggtcat aaagatgtcc tgaacgatga ccagttccag atcttcaaag gctttacgta
    14401 ctgccgacag ctccgcatca gtttgcagcg gatcttcgcc cataatgtac gcggcacgca
    14461 cttcgccatg cgctgcgcgg tgcggcagct cgctgatgcg atagccggta tgagcaggca
    14521 ggctttccac gccccaggct ttggcgaatt tctcgcggtt agccggatct ttcacgtact
    14581 ggtatcccgg atacgtatcc ggcagcgcgc ccatatcgca ggcaccctgc acgttgttct
    14641 gaccacgaac cgggtttaca cccgcatgcg gcttaccgag gttaccggtc agcatcgcga
    14701 ggctggtcag agaacgcacg gtttccacgc cctggtagaa ctgggttaca cccatgcccc
    14761 acaggatggc ggcgcttttc gcctgggcat acatccgtgc cgcctgacga atctcactgg
    14821 cgctgacgcc ggtgatatct tcaaccgact ccggcgtgta gccttcaacg attttacgat
    14881 actcttcaaa gccttctgta cgggaagcga cgaacgcttt gtcgtacaga ttttcttcaa
    14941 taatgacatg gcccatcgca ttcaacagcg cgatgttcga gccgtttttc agtgcaatgt
    15001 gcatgtcagc aatgcgcgcg gtttcaattt tgcgcggatc gcagacgata attttcgccc
    15061 cgttacgttt agcgttaatt acgtgattcg ccatgattgg gtgggaatcc gccgggttgt
    15121 acccgaaaac gaacactaaa tcggtattat caatttcgtt aatagcattg ctcattgcgc
    15181 cattaccgac cgattggtgc agacctgcaa ccgatgggcc gtgtcagaca cgagcgcagc
    15241 agtcaacgtt attggtacca ataacggcgc gcgcaaattt ttgcattaca tagttggttt
    15301 cgttacccgt accacgcgag gagccggtcg tctggatggc atccggaccg tacttctctt
    15361 tgatggcgct caggcgctcg gcaacgtaat tcagtgcctc atcccaggaa acaggttcga
    15421 gtttgccgcc acgctggcga cggatcatgg gggttttcag gcgcggggtc aggatctggg
    15481 tatcgttaat gaagtcccag ccataataac ccttcagaca cagggtaccc tggttggttt
    15541 tcccctgcgc tgcctccgcc cggacgattt tgccgttatc gacgaccagg ttgattttgc
    15601 aacctgatgc gcaatagggg caaaccgtga cgactttttt catcggtctc gctccagtta
    15661 atcaaatcac gcatacgcgc tctcgactac agtatgcatc ttttatgcca cattttattt
    15721 ggggtcattc cctgatatta cgggcactat ttattcaaaa ctctgacgaa aaacaggctg
    15781 tcgtcagttt tgacgtgacg aaacgaaata ccgcgtgaca gccatcacgc ggcagacatt
    15841 ttattttttc tcgacgacgg gagccgcctg atacccgccg cccagcgatt tcatcaactg
    15901 aatgctgtgg atcacccggc ggctgtccag catcagtaat gacatctctt cggcaagcac
    15961 tggcaaccgg gcttcggtgg cctgtaagcg gctggttaag ccgcgctgat aggcggcctc
    16021 ggcagcgcgc tgggtgaagc gcgttgcttc cacgcgttca gcctgcattt ctcgttcgtc
    16081 gttgagcgtt tgcagacgcg tgccgttgac ggcaacgtca cgcaccgcgt tcagtactga
    16141 ctggttgtaa cgttcaatca tcatgttgct ggcggcgcga gtgccttcga gattggcatt
    16201 caaccgtcca ccgtcaaaca gcggcaattt cagacccggg atgaagttga actggcgact
    16261 ggtttttttg aataaggtat ccaggtggat ggagtccaga ccgaaaaacg ctttgatatc
    16321 aaagctcggg tagaacagtg cccgcgcgga atccacctga tctaatgacg cctgaacata
    16381 ccagcgcatg gcttgcagat ccgggcgtct ggcgagcaac tcataagaga gtgtcgccgg
    16441 aatgccggtc tggactcgtg gtaatgccac cggtttgatc tccggcatat cgctggcccc
    16501 cgcgccaatc aatgcacgca gagattctcg cgtttcggtg atttgccctt tgacggcagc
    16561 aatttgttta tcgaccgcca gaatctgtgc ccgcgcgccg tggaaaggca cttgtgcttc
    16621 cagaccgtgc gccactttac tctggtgcgc tttcaccgcg taatcaatca catcgcgagt
    16681 ttgttctaac agatcgagca tctgatagct ggcctgcata ctgtaataaa gctgcgctat
    16741 gcccgtggtc agcgatagct ctactgctgc ggtttctgcc agcgcggcat tatgcgcgcc
    16801 aatggcggcg gcaaccgctg agcgatgcac accccacaaa tcaagatcca gtccggcaaa
    16861 caaacctact gtggcttccg tatagtacgg cccgtccata cccagtgagg gcgcatccat
    16921 cgcataaggg cttaaaaagc cgttcgccga gacgcgttgg cggttgagca tccctaacgc
    16981 tgcgacctgt aattgtgaac cggcatctaa caaatcggcc tgggactgcg ctttttcttc
    17041 ccgcagtttc gcttcggcga gggtgtgtga accacttagc gtccgttgga tcagcgcatc
    17101 cagctgcggg tcattgagtt gtttccacca ctgcgcctgc ggccagccgg agctggcaag
    17161 atgaatatcg tcggccagtt tgatttgttc cggtttgagc tgttgatgag gtgcagaatc
    17221 cttacgtacc agggcacagc cggaaatcag cgtcgtgctg ccgagaatgc tgcacaacag
    17281 cagacgtgaa agttgacgat tgatcattgc gtggctcctt gtgcctgttc cgtggcgggc
    17341 gttaatgccg gggctgtcca gtctggcaag cgggcgaaaa gctggcagac gtgttgctcc
    17401 tgttttaata aggtgggcag gatggcctgc gaggcgggtg tctcttcaag cgttatttga
    17461 ggagaacagc tgagtgcggt tgccagaccg gcagcgtatt tttccagggc gtcggcaaac
    17521 tgacctgctc tgtccggctg tagtgcgtta tccagcgcct gcgccgagtt ccaggtggca
    17581 tcccaggcgt gaagaagatc gcggccctga cgaataaccg tttgcgaacg ttcaatcagt
    17641 aaggcgcgtt cttcgctgtc cagttgacgc tccagcgcca cgcgttggca catctcttca
    17701 caggcattaa acgccgcatg cagacctata cgaatttgca gataagtgcg cagagccgtg
    17761 acttcctgct ggcgtgggat ccgcattact ttacttagca tacccagcgc accggctagt
    17821 ttttgcggca gagtgcgcgc ttcactttca ggccagacaa aggtgtaaat caccgcggac
    17881 accacggtac caatgatgat acccagggcg cgatcgcgaa tttccaccag gtcgtacact
    17941 gggccaaaaa cgttttcgag cgtggcgagc gcgaaggtga cgaccatctg tgtgccgata
    18001 taagaagagc gttcagagct ggtggcaatc catgcgccca acaggaaaat cggtgccagc
    18061 acaaacagca attcgacaat attgtccagc cagggcatga ccagtagcgt gaataacagc
    18121 gccagaatcg cgccgcaaaa ggccccgcca aaacgcagca ccatcttctg gtacgacgaa
    18181 ccgacatttg gattagcgac gatcacgcag gtcagcatac aggtgtgaat gccttcccag
    18241 tccacgccgc tgtaaaaggt gtaacagatc aaacaggcga gcaatgtttt taccgcgtag
    18301 cgtatatagt ctggattggt aaaagcatcg gcgaccattg atggcggttt ggcggcgggc
    18361 gttggcggcg tattcgggtt catctggccc agttgtaaca acgtctggca gatattctcc
    18421 aggttacatt cccgtgccgc caccgcttca ctttcactga tccgccagtc gctttgccag
    18481 cactgacctt cagcaacggc atgctgcagc ttgttgattt ctgaggctaa tttttgccga
    18541 aattcaataa ttgcctgaga atcagcaaaa gaggtgggat cgtagcgatt cagcgtcgag
    18601 taaatgtagg ttaccgttgc cacgcagctt tgccaccatg cgctttgagt tcgccagttg
    18661 gcatcgtccg cgagacaaaa gacattcagt ttttgcagcg ccagcgcctc tctttcaatc
    18721 cgcgtttcgg gtagcggtgc gaggctgtct gtcaggtgac taatggcatc atcaagccga
    18781 tcattaagcg cctgatgcat ttgcgaaatg gcacgactgg gaaaccacag cacgccgatt
    18841 aacgtcatca gcaaggttgg atagaggcca acaacgatac accacagcgt taagcgcacg
    18901 accacttccg gatagtcgag catggcgggg aaggtttgcc cgtaaatggc gacaatggcg
    18961 acagcgaaaa agaccagtcc cagccgatgg gtgcgcatca aaaacatgca gcccatcagg
    19021 atcggcccgg cgatgatcaa tcggatcaac ggttcgccgt atgaccattt gtagatcaaa
    19081 aacaggctgc cgatctccag caccgtggca accacaaaca agatcgccac aaatttggtg
    19141 taaaacgcgt tcgactgaat accgtaaaac agcactgcca gcgataacgc cacaaaaggg
    19201 atctcaaaag tcatcgagat cagaatcacc agcaggcagc ctacccagag ttgcacgatc
    19261 tgcggcacgc ggccgggtcg ccgctcgctt aactcttcat gaaagaacgc cagcagcctg
    19321 accaccggta atggcaggga gttgagcgcg ctcattattg cggctcaaga ttagcgactg
    19381 ccgaagcgcc gatgcggaac atttccgggt caggtttatc gaccatgatt ttgaccggaa
    19441 aacgctgggc aacgcggacc cagttaatag agcgagaaac tttcggcagg ccgcccagca
    19501 ccaggccgcc gtcattcggt agcacaccgt agccaatcga atccacttta ccctcgaagg
    19561 ttttgccgct gtcactcatc aggcgaatcg ttgcgggtgt acctgagcga atatttttca
    19621 gatcggtttc gcggaagttg gcgatcacgt accagtgacg agtgtcgatt agggtaaaaa
    19681 tagggcgcat ggcagaagca aattgcccga cggaggtttt gagggaaatg acccggccat
    19741 caaacggcgc gcgaacggtc gccatttcca gatgcagttt ggtcagggca atatccgctt
    19801 cgaccgccgc acgctgggca actaatacat ccacgccgct gacggcgctg gcggctgact
    19861 gcgcctgtaa caatacggca ttaagatccg cttctgcggc gcgctgcgcc gttcttgcac
    19921 ggtcaacatc ttccgctgag acaaaacctt ctttcagtaa tggctcggtg cggcgtaatg
    19981 tatctgtggc ctgtttcgcg gcggcacgtg ctttttctac cgtggcatta accgagtcgg
    20041 caccaaactg ttgggcgtca acgctacgct gggtgagcat aatttgctta tccagcgccg
    20101 cgagggaggc ttcagctttc gccagattgg cttcgtacgg gcgcgggtca atgcggaaca
    20161 gcaaatcgcc ctgtttgact gcctggttgt cggtgaccgc cagttctaca atgcggccgc
    20221 tgacttccgg caccacatca atggtatctg ctgacgcgta agcgtcatta gttgatggcg
    20281 cactgtctac gcgccagata acgaaaacaa gggcaaccag cgccaacgca accactaaca
    20341 gagcagggaa tttactgcga ggagcttttt tcggcgtact ttccatgagc gcgtctcaaa
    20401 tagattagaa aaatgccagc cagaacagca tggcgtagac agcaaaaagg gcggtataaa
    20461 taattccgac aaatgccaga ttgatatttg cccgctgaat aattcgcctc gtgattagcg
    20521 tcaaaataag gctggcaatg gcgcagaaaa accagctggg ataataagcg ccgatcaccg
    20581 ggattgccgg agataatgag catcccgaaa ccatgaagac gggaattatc caggctgttt
    20641 ttttgagttg catcgccatg cgagagagaa ctgttggcat attaaataca tcctgtttgc
    20701 tgcgaacaat atctgtagtg aatatcaata tccattgaag gcgtcagtat ggctattttc
    20761 ttgatgaata aaaatagctt tattcagcca ccgcattcat aacttatata ttttatgtgt
    20821 taattgattg tatgtatttt tataataaat gcttttgggg gcttaagcgc ccccaaaagt
    20881 gagtggcatt aatttggggt tacgatattc acccacaaat cgaaattatc cagacaggca
    20941 ataatttctt ccagtttcgc gccattgcca ataattttgg ctttcttcgc ttttaccaga
    21001 tccgccattt tggcttgtcc tgtcagcacg gcgtgcagat cttcacggct gatgtagaaa
    21061 gaggcgtcgg cttgcggttg cagtgtttta cggtagttaa gcacgctatc gttcagcgtc
    21121 aggttgaggt tatcgccgtt gctcatattg aagttcaggc tgatattttt acccgcagct
    21181 ttcgcgctat cgaggcgaac ggacataaag tcgaacagca tttcgaccga catcccgcga
    21241 atggtgtccg gggaaccggt ggtgccgtgg ctgaacttat gcaccccttc gcgcagctct
    21301 ttcgcgccgg tcaggtagaa accgcgccag gtggcggact cggcctgata gcccagctgt
    21361 tcaaagttat tcgcttgcag attcttcgcg acctggtcac ccgggttggc ggcaatcacc
    21421 tgtttcagca gttctgccga ccagcggtaa tcaccttgct tgttcgcttc ttgcgccagg
    21481 ctgatgacac gggcagaacc gcccagcgcc tgcacgtaac gtttacccat ctccacctga
    21541 ccatacggat gcaggttagc cgggttaccg tcgtaatagc caagatagaa gttatacacc
    21601 gcgcgggcgt tgtggctgac agaaccgtaa tagccacggc tggcccagtt attggcaagt
    21661 gcaggcggca gtttaatcat gtcgccgatt tcattcatgg tgtagccctg gttcgccagg
    21721 tgcagggtct ggtcgtgaat gtacttgata gtatcgcgat atttaccaat ataatcattg
    21781 atatgcttat tgccccggac cggccaggtg tgcggcataa acaggacttc cgcgtcgtta
    21841 ccccacatat ccagcgtttc gttcagatac tcggtccact tgctggtgtc gcgggttttc
    21901 gcgccgcgca gagtgtagaa gttgtgcagg gtatgcgtgg cgttctcggc ggtacacagc
    21961 gctttcaggg ccggaatata gaagtgcatt tcggctggtg cttcgctacc tggggtcatc
    22021 aggaagtcaa actccaggcc gtcgataatc atcttctcac cggttttggt gatggttttg
    22081 ttcggtgcga tgatgctcgg gtagcccgat gccagcgtta cgccaagacc attgccgatg
    22141 ttaccctgag ggttatgcgc cagcagcagg ccgtaggagt aaagtgcacg gtggctcata
    22201 atattacccg ccagtacgtt ttcgctgatg gcttcgtcca taaagccagc aggggcgata
    22261 acctgcactt taccggattt cacatcagct tcagagataa tgcctttcac gccgccatag
    22321 tggtcggtat ggctgtgggt ataaataacc gcaacaatcg gtttttgcgg gcgattttgg
    22381 aagtaaaggt caagggcggc ttttgcgctc ggtggcgtca ccagcgggtc gatgacaata
    22441 atcccggtat caccttcgat aaaggtgatg ttggagatat cctgaccgcg aacctggtac
    22501 attctgtcgg tgactttaaa caggccggaa ataccgttta actgcgactg acgccacagg
    22561 ctagggttaa cggtttcggg cgcgggcgca ttgatatcaa atttataatc ttccccg
//
LOCUS       DAJTMC010000057        22454 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.057, whole genome shotgun sequence.
ACCESSION   DAJTMC010000057 DAJTMC010000000
VERSION     DAJTMC010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22454
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.057"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            41..232
                     /locus_tag="OO130_001917"
     CDS             41..232
                     /locus_tag="OO130_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR5365350.1"
                     /translation="MKYGKLGYIVPRPQKIRGRWLIDRRAVFVGPGETGIAPEIHTGD
                     DDALKEILTHVTEATKKQH"
     gene            201..1307
                     /locus_tag="OO130_001918"
     CDS             201..1307
                     /locus_tag="OO130_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HCR5365351.1"
                     /translation="MSPRPRKNSTDVAGLYEKFDRRTGRVYYQYKNPVTGKFHGLGTD
                     KGKAEKIASTANQRIAAAEAEYFMRKIDESPSATKRRGIRLKAWVDRYLKIQDTRLKN
                     GDIAATTHKEKTRMAAYLVSRLGNHPLKELEVRDFALILDEWLDKDMVSTARVNRGLW
                     VDIYKEAQHAGEVPPGWNPPEATRKPIPKVTRARLTMEDWQKIYNATPEKHFIRNAML
                     LAIVTGQRRDDICHMRFSDVWNEHLHITQGKTGMRLALPLTLRCDAIGITLKEVIDGC
                     RDRILSPYLIHSRHQKQPKPMSKDNLSDYFAKARDLAGIIPPAGKTPPTFHEQRSLSE
                     RLYRAQGIDTKTLLGHKVQATTDRYNDTRGQEWV"
     gene            1487..2281
                     /gene="potC"
                     /locus_tag="OO130_001919"
     CDS             1487..2281
                     /gene="potC"
                     /locus_tag="OO130_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HCR5365352.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMISPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDETKGNTGDVK"
     gene            2278..3324
                     /gene="potD"
                     /locus_tag="OO130_001920"
     CDS             2278..3324
                     /gene="potD"
                     /locus_tag="OO130_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HCR5365353.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETCIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(3480..4301)
                     /gene="cobB"
                     /locus_tag="OO130_001921"
     CDS             complement(3480..4301)
                     /gene="cobB"
                     /locus_tag="OO130_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HCR5365354.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     KIQPNAAHLALAKLQEALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(4317..5228)
                     /gene="nagK"
                     /locus_tag="OO130_001922"
     CDS             complement(4317..5228)
                     /gene="nagK"
                     /locus_tag="OO130_001922"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HCR5365355.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCRGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQQLQAPEIIALYNQG
                     DEQARAHVERYLDLLAVCLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(5257..6501)
                     /gene="lolE"
                     /locus_tag="OO130_001923"
     CDS             complement(5257..6501)
                     /gene="lolE"
                     /locus_tag="OO130_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HCR5365356.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDKVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHVAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEMTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     LIIGVVVSLQLTPIIERIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(6501..7202)
                     /gene="lolD"
                     /locus_tag="OO130_001924"
     CDS             complement(6501..7202)
                     /gene="lolD"
                     /locus_tag="OO130_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HCR5365357.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(7195..8394)
                     /gene="lolC"
                     /locus_tag="OO130_001925"
     CDS             complement(7195..8394)
                     /gene="lolC"
                     /locus_tag="OO130_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HCR5365358.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRMMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGNEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEDSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMIFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPMAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            8656..9729
                     /locus_tag="OO130_001926"
     CDS             8656..9729
                     /locus_tag="OO130_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HCR5365359.1"
                     /translation="MKQKELWINQIKGLCICPVVIYHSVITFYPHMTTFPHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQIELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            9857..13303
                     /gene="mfd"
                     /locus_tag="OO130_001927"
     CDS             9857..13303
                     /gene="mfd"
                     /locus_tag="OO130_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HCR5365360.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTKHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDVARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            13447..14409
                     /gene="ldtC"
                     /locus_tag="OO130_001928"
     CDS             13447..14409
                     /gene="ldtC"
                     /locus_tag="OO130_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HCR5365361.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPAGSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     TRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(14492..14749)
                     /gene="bhsA"
                     /locus_tag="OO130_001929"
     CDS             complement(14492..14749)
                     /gene="bhsA"
                     /locus_tag="OO130_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HCR5365362.1"
                     /translation="MKNVKTLIAAAILSSMSFASFAAVEVQSTPEGQQKVGTISAKAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            14990..15622
                     /gene="comR"
                     /locus_tag="OO130_001930"
     CDS             14990..15622
                     /gene="comR"
                     /locus_tag="OO130_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HCR5365363.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(15684..16223)
                     /locus_tag="OO130_001931"
     CDS             complement(15684..16223)
                     /locus_tag="OO130_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HCR5365364.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(16450..17754)
                     /gene="ndh"
                     /locus_tag="OO130_001932"
     CDS             complement(16450..17754)
                     /gene="ndh"
                     /locus_tag="OO130_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HCR5365365.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHSELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTHDPDIYAIGDCASCTRPEGGFVPPRAQAA
                     HQMATCAMNNILAQINGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(18154..18696)
                     /locus_tag="OO130_001933"
     CDS             complement(18154..18696)
                     /locus_tag="OO130_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR5365366.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(18719..19744)
                     /gene="nagZ"
                     /locus_tag="OO130_001934"
     CDS             complement(18719..19744)
                     /gene="nagZ"
                     /locus_tag="OO130_001934"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HCR5365367.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPEKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSTLIRENKLDAIMPAHVIYSD
                     VDPRPASGSSYWLKTVLRQELCFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKASSTRLNQLHERW
                     QEEKAGH"
     gene            complement(19755..20579)
                     /gene="thiK"
                     /locus_tag="OO130_001935"
     CDS             complement(19755..20579)
                     /gene="thiK"
                     /locus_tag="OO130_001935"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HCR5365368.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDTALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(20563..21201)
                     /gene="lpoB"
                     /locus_tag="OO130_001936"
     CDS             complement(20563..21201)
                     /gene="lpoB"
                     /locus_tag="OO130_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HCR5365369.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDRTAPPAPHIRHYDWNGAMQPIVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQ"
     gene            complement(21215..21592)
                     /locus_tag="OO130_001937"
     CDS             complement(21215..21592)
                     /locus_tag="OO130_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HCR5365370.1"
                     /translation="MRKGCFGLVSLVLLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQKPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(21595..21954)
                     /gene="hinT"
                     /locus_tag="OO130_001938"
     CDS             complement(21595..21954)
                     /gene="hinT"
                     /locus_tag="OO130_001938"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HCR5365371.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVVAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
BASE COUNT         5592 a         5893 c         5576 g         5393 t
ORIGIN      
        1 cgacgagtgg aaaccaaaac ggttcagtct cactaccctg gtgaaatacg gaaaactagg
       61 atacatcgtt ccaagaccac aaaaaattcg tgggcgttgg ctgatagatc gccgagcagt
      121 atttgttggg ccaggtgaaa cgggaattgc gccggaaatt catactggcg atgatgatgc
      181 actgaaggag attttaactc atgtcaccga ggccacgaaa aaacagcact gacgtagccg
      241 gtctttacga aaagtttgat cgcagaactg gcagagttta ctaccagtat aaaaatcctg
      301 tgactggaaa atttcacgga ctcggaacag acaaaggtaa ggcagaaaaa atcgcttcca
      361 cagccaatca gcgaatagct gcagcagaag ctgaatattt catgcgcaaa attgatgaaa
      421 gtccgtcagc aacaaaacgt cggggtatca gattaaaggc atgggttgat cgatatctga
      481 aaatacagga cacgcgactg aaaaatggag atattgcagc tacaactcac aaagaaaaaa
      541 ctcgaatggc tgcatacctg gtttcccgtc tgggaaacca cccattgaaa gaactggaag
      601 taagagactt tgcattaata ctggatgagt ggctggataa agacatggtc agcacagcga
      661 gagtaaatcg tggattatgg gttgatattt ataaagaagc acagcatgca ggggaagttc
      721 ctcctggatg gaatcctccg gaggctaccc gtaaaccgat ccctaaagta accagagcca
      781 ggctcaccat ggaagactgg caaaaaattt acaatgcaac gcctgaaaaa cactttatcc
      841 gtaacgcaat gcttcttgcg attgttactg gtcagcgccg tgatgacatt tgccacatgc
      901 gtttttcaga tgtgtggaac gaacacttgc atatcaccca gggaaaaacc ggaatgcgtc
      961 tggcgttacc gcttacacta cgctgtgatg ccattgggat aacgttaaaa gaagttattg
     1021 atgggtgccg agacagaata ttaagtccat atctaatcca tagtcggcac cagaaacaac
     1081 cgaagccgat gagtaaagac aacctgagcg actactttgc caaagcacgg gatctggctg
     1141 ggataattcc accagcagga aaaactccgc caacatttca tgaacaacgc tctctatcag
     1201 aacggctgta ccgtgcacag ggtatcgata caaaaacatt actaggacat aaagtccagg
     1261 caaccaccga tcgctataac gatactcgag gtcaggaatg ggtttaagct ggttatttga
     1321 caaacaccat atggaatatc cgtcaccatc tcaggaaaaa agtgttgtaa aatgcgtggc
     1381 ctagttttgg ggaaacgttt tggagagatt ttggagaaag gaaaaaattg aataaattca
     1441 aaatcctgaa agtgctccac cttatgaata agaaggtgga actcgaatga tcggtcgact
     1501 gcttcgcggc ggttttatga ccgctatcta cgcgtacctg tatatcccaa tcattatttt
     1561 gattgtgaac tcctttaaca gctcgcgctt tggcatcaac tggcagggtt ttaccaccaa
     1621 atggtatagc ctgctgatga acaacgacag cctgttacag gcagcgcagc attcactaac
     1681 aatggcggtg ttttcggcga cgtttgctac gcttatcggt tcactgacgg cagttgcgct
     1741 gtaccgttat cgctttcgcg gtaagccgtt cgttagcgga atgctgtttg tggtaatgat
     1801 atcaccagat atcgtgatgg caatttcgct gctggtgctg tttatgctgc tgggtattca
     1861 gcttggcttc tggtcgctgc tgttctcgca tatcaccttc tgcctgccat ttgtggtggt
     1921 gacggtgtat tcgcgcctga aaggttttga cgtgcggatg ctggaagcgg cgaaagatct
     1981 cggtgccagc gaatttacaa ttctgcgaaa aatcatactg ccactggcaa tgccagcggt
     2041 ggcagcgggc tgggtgttaa gctttaccct gtcgatggac gatgtggtag tttcttcgtt
     2101 cgtcaccgga ccaagttatg aaattctgcc gttaaaaatt tattcgatgg tcaaagtcgg
     2161 cgtatcgccg gaagttaacg cgctggcaac catattactg gtgctgtcgc tggtgatggt
     2221 tattgccagt cagcttattg ctcgtgatga aacgaaaggt aacacagggg acgttaaatg
     2281 aaaaaatggt cacgccacct gctcgcggcg ggtgctctgg cactgggcat gagcgccgct
     2341 cacgccgatg acaacaacac gctgtatttc tacaactgga ccgagtacgt gccgccagga
     2401 ctgcttgaac agttcaccaa agaaacctgt attaaggtta tctattcgac ttacgagtcg
     2461 aacgaaacca tgtacgccaa gctgaaaacg tacaaagacg gtgcgtatga cctggtggtt
     2521 ccttcaacct attacgtcga taaaatgcgt aaagaaggga tgatccagaa gatcgacaag
     2581 tcgaagttaa cgaacttcag caatctcgat ccagacatgc tcaacaagcc ttttgacccg
     2641 aataacgact actccattcc gtatatctgg ggggcgacgg cgattggtgt taacggtgat
     2701 gcggtggatc cgaaatctgt caccagctgg gccgatctgt ggaagccaga gtacaaaggc
     2761 agcctgctgt tgaccgacga tgcccgtgaa gtgttccaga tggcgctgcg taagctgggc
     2821 tattccggta acaccaccga tccgaaagag attgaagctg catataacga gctgaaaaaa
     2881 ctgatgccaa acgtcgcagc gtttaactcc gataacccgg ctaacccgta catggaaggc
     2941 gaagttaacc tcggcatgat ctggaacggt tctgctttcg ttgcacgcca ggcgggtacg
     3001 cctattgacg tggtgtggcc gaaagaaggc ggcatattct ggatggacag cctggcgatc
     3061 ccggcaaatg ccaaaaacaa agaaggcgcg ctgaaattga tcaacttcct gctgcgcccg
     3121 gatgtggcaa aacaggttgc tgaaactatc ggttatccaa cgccaaacct tgcggcgcgt
     3181 aagctgttaa gtccagaagt ggcgaacgat aaaacgctct acccggatgc tgaaaccatt
     3241 aaaaatggcg aatggcagaa tgacgttggc gcagccagca gcatttatga agagtattat
     3301 cagaagctga aagcaggacg ttaattcagc aagcgggcgg tggtattacc gcccgttttt
     3361 tatttagcaa ggatgtggct aatgagtcag gacagtagag ttttatccga ctctttcgtc
     3421 ggtattgtgg tccgctcgta ggcctgataa gcgtagcgca tcaggcaatg cttcctgctt
     3481 tataatccct tcagcaactt ttcaacaaac tcaggcacca cctggcttgc cgggccgtaa
     3541 tatttctcgg caaattcatt accgacctga cttggttcga gattcagttc cacggtgtgc
     3601 gcgccatgca gtttcgcttc gtgaacaaac ccagccgccg gataaacatg cccggaagta
     3661 ccaatggcaa tgaaaatatc agccatcgac aacgccatat aaatttcatc catgccgagt
     3721 ggcatttcgc caaaccaaac gacgtgcggg cgcaagggtg ccgggaactg gcaacaatgg
     3781 catttatctt ctggcgtaac gtctccggtc cagtcgagaa cctgaccact ttgcgaacaa
     3841 cgaactttca gcaattcccc atgcatatga atgacattgg tattacctgc gcgttcatgc
     3901 aggttgtcta tattctgcgt taccagcaaa aagcgatcgc cgagagcctc ttgcagttta
     3961 gccagcgcaa gatgcgcggc gttaggctga atttttggct gctgcagctg tcgacgacgg
     4021 gcgttataaa acgcttgcac cagttcagga tcgcgatcga aaccttccgg cgttgccaca
     4081 tcttcaaccc gatgttcttc ccacaggcca tctgcggcgc gaaaggtacg aatacctgat
     4141 tccgcagaaa ttcctgcccc tgtcagtacg agtactcttg gtttttccat tgcttccggc
     4201 accactttat ctctgaaaaa aatacgctga cgcaaacgct cgcgcaggcg gcgtttattt
     4261 ttacgaaaac gacttaaccg atgaccccga cgcgacagca tagcaacctc tgttgtttaa
     4321 tcggttagat gtaggaaggc cgcaccacgc attcctcccg catcaccgtg gcgcgcgcgt
     4381 tcaatgcgcg gaacacgagc tacaggtaag agatgacgag gcagcctgtc cgccagttgc
     4441 gttgtgattg ccgggaaatt cgataagcca ccaccaatga cgaccaggtc agggtcaaca
     4501 atggtcagga tatttcccag acaaaccgct aataaatcca gataacgctc aacgtgcgcc
     4561 cttgcctgct catcgccttg attataaagc gcaataattt cgggagcctg caactgttga
     4621 tgataatagt gttgatacag ccacgcaaaa ccgcgaccag acagataatt ctcaatgcag
     4681 ccatgctgac cacagccgca gcggcgtaac gggaaatcca accccatcat ggttaacgca
     4741 tcaaccggca gacgcatatg gccaaactcg ccggtaatgt agcttttacc ggtaatcggt
     4801 ttgccgttga aaatcagccc accgccaacg ccggtaccaa gaatcaaccc catcaccagt
     4861 ggatattgcg taaattcatc atcccaggct tctgaaaggg caaaacagtt ggcatcgtta
     4921 tcaaggcgta catcgcgatc aagacgtgcg ctcaggtcgg cacgcagcgg tttaccgctg
     4981 gcagcgggga cattggcggc atacagcgta ccatcttctg tttccggcat tcccggtata
     5041 ccaatgccga cagaacctct acagccaaaa cgttgatcag cttcggctac cagctcgcac
     5101 actgcatcta aaaatgcgtc atagctgtca cgcggtgtcg gcacccgctt ttcccactgc
     5161 aactgccgac cgctatcaaa cacgccaagc gcaatttttg ttccaccaat atcaaacccg
     5221 taatacattg ccgctccttg ttttaatgta ctgcctttac tggccgctaa ggactcgcgc
     5281 agggtcaata ttactggcgc gccgcgccgg ataccaactt gccaaaagac tcagcaacaa
     5341 tgctgtgacc agtacgtaga agacgtccag ccagtgcaat tccgatggca ggaagtcaat
     5401 aaaatagata tcgctggaga ggaactgatg accgatcagc ttttcaatcc gctcaataat
     5461 cggggtaagt tgcagtgaaa caactacgcc gataatcagc ccacacaggc tgccgaacag
     5521 ccctgccagc aatccatacc agacaaagat ggcgcgaatt aaaccatctt tcgcccccag
     5581 cgttcttaat actgcgatat cgccactctt atctttcacc gccatcacta aggtggagac
     5641 gatgttgaaa caggccacgc caatcaccag taccatcgcc agatacataa tggcgcggat
     5701 catctggata tcgcgataca tatagccgta agtaccaatc cagcttttaa tataaacata
     5761 gctgttggtc atttcacccg catcgcgtac cagcttattg gcgttgaaaa catccgtcat
     5821 tttaagggca atacctgaca cgctggaacc catatcaaga tattgttggg catccgccag
     5881 cgggatcatg gcaaaactgt gatcgagttg gccactcaac tgcaaaatac ccgcaacgtg
     5941 caaacgcaca cgttttggct gcatcagttt atgctcagga ttcgagttgg ggatcatgat
     6001 cgacacccaa tcgccctgct tcactttcag cgcatccgcc acgcctttgc cgatgataat
     6061 ttgctgttcg cccgctttaa aattgcgcca cgcatccccc tgaacaaacg agggtaatgc
     6121 gctcagacgc tgttcctgtt gtgggttaac gcccttcacc tggattgcgc gcagattcgc
     6181 tccactttcc accagcccgg tgaaattgat atacggcgca gcggcggcaa tacccggcac
     6241 tttctgcact ttatccagtg cttcctgcca gttagtccat ggctgattta ccgcttcgat
     6301 ttcaccatgc ggcaccactg ccagaatgcg gttattcagt tcgcgttcaa agccgttcat
     6361 cgcgcttaag ccgacgatca ataccgccac accgagggca atgccaatgg tagaaatgac
     6421 ggagatcagc gacaccatgc cgccgcgccg ccgtccgcga ctaaaacgca ggccaattaa
     6481 taacgataaa ggcatcgcca ttactccgcc cccatcaggc tcagttccgc cgtcagacgt
     6541 ccatcacgca tttctaactg gcggctcata cgtttcgcca gttgcaggtc gtgagtaacc
     6601 accaggaagg cggtgccctg caagcgattc aattccccaa gcaactggaa gatgctgtcg
     6661 gcgttacgcg catcgaggtt accggtaggc tcatccgcca gtaccagacg cggattattg
     6721 accagcgcac gggcaatcgc tacacgctgg cgttcgccgc cagaaagttc agatgggcgg
     6781 tgattcgcac gatgctccag ccccaccgct tttaacatct ccagtgcacg gctgttgatt
     6841 tcagcgggct ttttcttgcc aatcagcagc ggcatagcca cgttttccag ggcggtaaaa
     6901 tccggcagca ggtggtgaaa ctgataaata aagcccagtt tctggttgcg cagttctgct
     6961 tttgctgccg acgacagttt gctcatcggt tgaccgttaa agatcacatc gccggaggtt
     7021 ggcgtatcca gtccgcccag caggtgcagc aaggtgcttt taccggaacc agagctaccg
     7081 acgatcgcca tcatttcacc ttcgccgacg ctgaaactga cattgtgcag tacatcggtt
     7141 tgcacactgc cttcctgata gcgtttgcac aggttgtcgc attgcaacag gatcttattc
     7201 ataacgtaaa gcctcagcgg gttgtgtggc ggcagcgcgc catgaagggt aaagcgtaga
     7261 cagcagcgcg atagccatcg ccaccagcgc aataacaatg acctgtaaag gttcgatagc
     7321 catcggcagc gccgcgccat caagcaggac gccgattatt ggcatcagat tatttaactg
     7381 gctggcaagc agtgcgccaa gcgccgctcc gaggatcgca ccgataatcc cggcgctggc
     7441 cccttgcacc ataaagatca tcatgatttg tcgcggagtt aagccttgcg tttgcaggat
     7501 cgctacttcg ccctgcttct ccatcaccat cagccccagc gaggtaataa tgttaaacgc
     7561 cgcaacggcg acaatcaggc tcagcagtaa acccatcata tttttttcca tgcgtacggc
     7621 ctggaacaac tcgcctttac gatcacgcca gtcctgccat ttgctgtctt caggcagttt
     7681 ttgctgactt aatgagtcga ctttcagcgg ctcatccagc cacaaacgcc agccggtaat
     7741 attgcctgcc ggataacgca tcagacgcga ggcatcctca atattcacca gcatttcatt
     7801 gccatcgact tcactgttgg cggcgaaagt gccaatcaca ttgaacaggc gctgacttgg
     7861 aatacgcccc atcggcgtga actggctggc agatggtacc atcatgcgga tttgatcacc
     7921 gcgattaacg cctagctgtg aggcaagttg ttcgccgagg atgacattat atttccccgg
     7981 ctcgaggtca gtttgtttca cattgaccag atacggcgtt agtggatctt tttgcgccgg
     8041 atcgataccc agcatcaccc cgaccgccac gctgcgcgcg ctttgcagta ccacatcacc
     8101 ggtagtaata ggtgcgacgc gattaacgcc gtccagtttg actgccgttt ccgggagttg
     8161 ctgcgggtta agagagccat gctcagaaga gagaattgcc tgtggcatca ggccaaggat
     8221 gttgttttgc agctcgcgct caaagccgtt catcactgac aatactgtga ccagcgccat
     8281 caccccgagg gtaatgccga tggtagaaag ccaggagacg aaacgaccga agcgatccgc
     8341 tgcacgccca cgcatgtaac gcaggccaat aaatagagcg acaggttggt acatgaaatc
     8401 cgtctggttg ctgtagcaaa gacacgagta tataagcgaa agcaagatgg gtaaacgtct
     8461 gaactgtaag tgatcgtcag ggctaatctg aaaagccccc gtaaatcaat cggctaaatt
     8521 gccatgtttt atcatcaatc ttgccgcctg atcttaatct gaaaaatcat ccaatacaga
     8581 cagataccca ttacacctcg ggtcataacc cgcaggatca accgcatgta aattaaaaaa
     8641 gagcgtgttg tcctgatgaa acaaaaagag ctatggatta accagatcaa agggttatgt
     8701 atttgtccgg tggtgattta tcactcggtc attacctttt atccgcatat gaccactttt
     8761 ccgcatccgt tatcggaagt cctgagcaaa tgctggatct atttcaatct ttaccttgca
     8821 ccctttcgta tgccggtttt tttctttata tccggctatt tgattcgccg ctatatcgac
     8881 agcgtaccgt ggggaaattg tctcgataaa cggatctgga acatcttctg ggtgctggcg
     8941 ctttggggcg tggtgcagtg gctggcgtta agtgcgctga accagtggct ggcacctgag
     9001 cgcgatttaa gcaatgcctc caatgccgct tatgccgatt ccaccggtga gttcctgcac
     9061 gggatgatca ccgccagcac cagcttgtgg tatctgtatg cgttaattgt ctatttcgtg
     9121 gtatgtaaaa tttttagccg tctggcgctg ccactattcg ccttgtttgt actgctgagt
     9181 gtggcggtta atttcgttcc cacgccgtgg tggggaatga acagtgtgat ccgcaatttg
     9241 ccttattaca gccttggcgc atggtttggc gcaacaataa tgacctgtgt taaagaggta
     9301 ccgttgcgcc gccatctgct gatggcttct ttgcttaccg ttctggcggt cggtgcctgg
     9361 ttgtttacta tctcgctgct gttgtcgctg gtatcgattg tggtgatcat gaagctgttt
     9421 tatcagtacg agcaacgatt cggtatgcgc tccaccagcc tgctgaatgt gattggctcc
     9481 aacaccattg ctatctacac cacccatcgc attctggttg aaatattcag cttaactctg
     9541 cttgcgcaaa tgaacgcagc acgctggtcg ccgcaaatcg aactgacact cctgctggtt
     9601 tatccctttg tcagtttgtt catctgtact gttgcgggct tgctggtaag aaaactttca
     9661 cagcgtgcat tcagcgatct gttgttctcc ccgccttcac tgcccgctgc cgtcagttac
     9721 tcccgctaag ccatcgcccc agccggggcg attcaatttg ctaaaccatg tcgattacgg
     9781 ataatgaaca gcattgctta tcagagatat gcccccatat gttgaggcat atcctaacga
     9841 gaatctgaca accgttatgc ctgaacaata tcgttatacg ctgcccgtca aagcgggtga
     9901 gcagcgtctg ctgggcgagt taaccggcgc agcctgtgca acgctggtag cggaaattgc
     9961 cgaacgtcac gccggtccgg tggtactcat tgcaccagat atgcaaaatg ctctgcgttt
    10021 gcatgatgaa atcagtcagt tcaccgatca gatggtgatg aatctggcgg actgggaaac
    10081 tcttccctac gacagctttt cgcctcatca ggacattatc tcctcgcgcc tttccaccct
    10141 ttaccagcta ccgacgatgc agcgtggcgt actgattgtt ccggtgaata cgcttatgca
    10201 gcgcgtttgc ccacacagtt ttctccacgg tcatgcgctg gtgatgaaaa aaggtcagcg
    10261 cctgtcacga gatgcattac gaacccaact ggacagcgcc ggttatcgcc atgttgacca
    10321 ggtgatggag cacggcgaat acgccacgcg cggcgcgttg ctggatctct tcccgatggg
    10381 gagtgagctg ccttatcgtc ttgatttctt tgatgatgaa atcgacagcc tgcgggtgtt
    10441 tgacgtcgac agccagcgca cgctggagga agtagaagcg atcaatctgc tgcccgcgca
    10501 cgaatttccg accgataaag cggcaattga actgttccgc agccagtggc gcgatacctt
    10561 cgaagtgaag cgcgatccgg aacatattta ccagcaagtg agtaaaggca cattacctgc
    10621 cgggatcgag tactggcagc cattgttctt cagcgaacca ctgccgccgc tgttcagtta
    10681 tttccctgcc aataccttgc tcgtgaatac tggcgatctg gaaaccagtg ccgaacgttt
    10741 ccaggctgac acgctggcgc gttttgagaa tcgcggcgtc gatccgatgc gcccgctgtt
    10801 gccaccacaa tcgctctggc tgcgggtgga cgagctcttc tcagagctga aaaactggcc
    10861 ccgggtgcag ctaaaaacta aacatttacc gacaaaagcc gcgaatgcca atttaggttt
    10921 ccagaaactg ccagacctag ccgttcaggc gcaacaaaaa gcgccgctgg atgcgctgcg
    10981 taagttcctc gagactttcg acggtccggt ggtgttctcg gtagaaagtg aaggtcgccg
    11041 tgaagcgctg ggtgaactgc tcgcacgaat taaaattgct ccgcaacgca ttatgcgtct
    11101 tgatgaagcc agcgaccgtg ggcgttatct gatgattggc gctgccgaac atggttttgt
    11161 cgataaggtg cgtaatctgg cgctgatctg cgaaagcgat ctgctcggtg aacgtgttgc
    11221 ccgccgtcgt caggattctc gccgcaccat caaccccgat acactgatcc gtaaccttgc
    11281 ggagctgcat attggtcagc cggtggtcca tctggagcac ggtgtcgggc gctatgccgg
    11341 aatgaccacg ctcgaagcgg gcggcattac tggcgagtat ttgatgctca cctatgccaa
    11401 cgacgccaaa ctgtatgttc cggtgtcgtc actgcatctg attagccgtt acgcaggtgg
    11461 cgcggaagaa aacgccccgc tgcataaact tggcggcgat gcgtggtcac gcgcgcggca
    11521 gaaagcggcg gaaaaagtgc gtgatgtggc ggcggaattg ctggatatct acgcgcaacg
    11581 cgccgccaaa gagggcttcg cgtttaaaca cgatcgtgag cagtatcagt tgttctgcga
    11641 cagcttcccg tttgaaacca cgccggatca ggcgcaggcc attaatgcgg tacttagcga
    11701 catgtgtcag ccgctggcaa tggatcgtct ggtgtgcggc gatgttggct ttggtaaaac
    11761 agaagtggcg atgcgcgccg ctttcctggc agtagataac cacaagcagg tggcggtgtt
    11821 ggtgcctacc acccttctcg cgcagcagca ttacgacaac ttccgcgacc gtttcgccaa
    11881 ctggccggta cgtatcgaaa tgatctcccg tttccgcagc gccaaagagc agacgcaaat
    11941 ccttgcggaa gtggcggaag ggaaaatcga tattctgatc ggtacgcaca aactgctgca
    12001 aagtgacgtc aagtttaaag atttaggcct gctgattgtc gatgaagaac accgcttcgg
    12061 ggtgcgtcat aaagagcgca ttaaagcgat gcgcgcgaac gtggatattc tgacgcttac
    12121 tgcaacgccg atcccacgca cgctgaatat ggcaatgagc ggaatgcgtg acctgtcgat
    12181 tatcgccacg ccgcccgccc gtcgtctggc agttaaaacc tttgtccgtg agtatgacag
    12241 cctggtggtc cgggaggcga tcctgcgtga aattttgcgc ggggggcagg tttattatct
    12301 ctacaatgat gtggaaaaca tccagaaagc tgccgaacgg ctggcagaac tggtgccaga
    12361 agcgcggatc gccatcggtc acgggcagat gcgcgagcgc gaactggaac gggtgatgaa
    12421 tgatttccat catcaacgtt tcaacgtgct ggtttgtaca accattatcg aaaccgggat
    12481 cgacatcccg acagccaaca ccattatcat tgaacgcgcg gatcacttcg gtctggcgca
    12541 gctgcaccag ttacgcggtc gcgtcggacg ttcacatcat caggcatatg catggctgct
    12601 gacgccgcat ccaaaagcga tgactaccga tgcacaaaaa cgtcttgaag cgattgcctc
    12661 gctggaagat ctcggtgcag gctttgcgct ggcaacgcac gatctggaga tccgcggcgc
    12721 gggtgaactg cttggcgaag aacaaagtgg ctcaatggaa accatcggtt tctcgctgta
    12781 tatggagttg ctggaaaacg ccgtcgatgc actgaaagcc ggacgcgagc cgtcgctgga
    12841 agatctcacc agccagcaaa cagaagtcga gctgcggatg ccgtcgctat tgccagatga
    12901 tttcattcct gacgtgaata cgcgtttgtc gttctataaa cgtattgcca gcgccaaaac
    12961 ggaaaacgaa ctggaagaga tcaaagtcga gcttatcgat cgcttcggcc tgctgccgga
    13021 tccggcgcgt accctgctgg atgttgcccg tctgcgccag caagcgcaga aactggggat
    13081 caggaagctg gaaggtaatg agaaaggcgg ggtgatcgaa tttgccgaga agaatcacgt
    13141 taatccggcc tggttgattg gtttgctgca aaaacagccg cagcattacc gccttgatgg
    13201 tccgacgcgc ctgaaattta ttcaggattt gagtgagcgg aaaacgcgta tcgaatgggt
    13261 acgccagttt atgcgtgaac tggaagagaa cgcgatcgct taatggtaaa tcgggggcgt
    13321 ttctgcgccc ccatcggcaa catttacaaa ttctttgcac ttccctgcac tatccgacac
    13381 tgtcaccatc cataattcag gcttatctgt ttattacaat aaccttatat ttattatgga
    13441 tttttggtga tgatcaaaac gcgtttttct cgctggctaa cgttttttac gttcgccgct
    13501 gccgtggcgc tggcgctacc ggcaaaagcc aacacctggc cgctgccgcc agcgggcagt
    13561 cgtctggttg gcgaaaacaa atttcatgtg gtggaaaatg acggtggttc tctggaagcc
    13621 atcgccaaaa aatataacgt cggctttctc gctctgttac aggctaaccc cggcgttgat
    13681 ccttacgtac cgcgcgcggg cagcgtgtta acgatcccgt tgcaaaccct acttccagac
    13741 gcgccgcgcg aaggcattgt gatcaacatt gcggagctgc gtctctatta ctacccgccg
    13801 ggtaaaaatt cggtaaccgt gtatccaata ggtattggtc agttaggtgg tgacacgctg
    13861 acaccgacaa tggtgaccac cgtttcagac aaacgtgcaa acccaacctg gacgccaacg
    13921 gcaaacaccc gcgcccgtta taaagcacag ggaattgagt tgcctgcggt agtgccggct
    13981 ggaccggata acccaatggg ccatcatgcg attcgtctgg cggcctatgg cggcgtttat
    14041 ttgcttcatg gtacgaacgc cgatttcggc attggcatgc gggtaagttc tggctgtatt
    14101 cgtctgcggg atgacgatat caaaacactc tttagccagg tcaccccagg caccaaagtg
    14161 aatatcatca acactccgat aaaagtctct gccgaaccaa acggtgcgcg tctggttgaa
    14221 gtacatcagc cgctgtcaga gaagattgat gacgatccgc agctgctgcc aattacgctg
    14281 aatagcgcaa tgcaatcatt taaagatgca gcacaaactg acgctgaagt gatgcaacat
    14341 gtgatggatg tccgttccgg gatgccggtg gatgtccgcc gtcatcaagt gagcccacaa
    14401 actctgtaaa tcctggataa aaaaaatccc cgcgacacgg catgtcgcgg ggaaaaaatc
    14461 agcagtagca ggcattaatg agggttaatg cttatttata aattactgct gttccatgga
    14521 gggtattcgg accggttaca gaagtaatac ggaaagattt tgcacccatc tcatccgctt
    14581 tttgcgccag ctgctcttcc agcgatccca gatttgtccc cgctttagca ctgattgtac
    14641 cgactttttg ttggccttct ggcgttgact gaacttcgac agccgcaaag ctggcaaatg
    14701 acatggagct taaaatcgcc gcagcgatga gggtttttac gtttttcata atagtggcct
    14761 tatgcagatg aatgacgtcg caagcaatta cttaacgatc gataactaaa tgacagatgt
    14821 gatctggatc acatacaaga tattttttat aacaatcatt aattaattta aaaatcttta
    14881 aatttcagtt aaatagaaag caattatttt tatgggatct ggactggtga acggaggtga
    14941 ttatttttat aataatcttt cattcacagt caccaggtac aacggcatca tggcaactga
    15001 ctcaacacaa tgtgtaaaaa aaagccgtgg ccgcccaaaa gtgttcgaca gggatgccgc
    15061 gcttgataag gccatgaaat tgttctggca acacggttat gaagcgactt ctcttgcgga
    15121 cctcgtcgaa gcgaccggag ccaaagcgcc cacgctatac gcggaattta ccaacaaaga
    15181 ggggttattt cgtgccgttc tcgaccgcta tatcgatcgt tttgccgcta agcatgaagc
    15241 acagctgttt tgtgaagaga aaagcgtgga gtctgcgctg gctgactatt ttgctgccat
    15301 cgccaactgc tttaccagca aagacacccc ggcaggctgc ttcatgatca acaactgcac
    15361 caccctctcc ccagattcag gagatatcgc caatacgttg aaatcacgcc atgcgatgca
    15421 agagcgcact ttgcagcagt ttttatgtca acgacaagcg cgcggggaaa tcccgaccca
    15481 ctgtgacgtg acacatctgg cagaattcct taattgtatt attcagggga tgtcgatcag
    15541 cgcacgcgaa ggtgcatcgc tggaaaaact aatgcagatt gccggaacga ctttgcgttt
    15601 atggcccgaa ctggtgaaat aagtcgcaaa aaaagcccct cagcctgagc gaatgagggg
    15661 ccaaattgca gagtacagcc ttgttatact ttgttattca aaatcagttg cccattacta
    15721 tctagcggga tctgcgtacc cggatcgcgg tccatgcgga ttttgccctg ctgatcgcca
    15781 atcttatagg tcacatcata accgagcatt ttttctgact tgtcatacac cgttttacaa
    15841 cgctgttgcg tagtcgtgta agtatcgctt tcctggagag agccctggat ctggttaccg
    15901 gcatatccac cacccagcgc ccccacaaca gtggcgacat ctttaccgcg accaccgcca
    15961 aactgatgcc ctatcacgcc gccagcaaca gcgccgagca ccgacccggt aatgcgattt
    16021 tcatcctgca ccggtcgacg atgggtcact gtgacgttgc gacactcctg acgcggtgtt
    16081 ttaaccgttt ccttgattgg ggttgcagaa acaacctgag cgtattgcgg gccacgttca
    16141 aacacgttca gactggccac tgccgctacg cccagcgcag ctgcgacacc aatcccgata
    16201 cccgccaaca ttgatttatt cacgggaaca cctccttctt tgcagttatg ccaatcatgc
    16261 aagcgaagga gaaaagcagc tatcagacag aagatgaaaa gcgcgggaat ggctggaaat
    16321 acttaatgat tcggaggagt ctgaaggaat gcctgatgcg acgcttaacg cgtcttatca
    16381 ggcctaccgc tcgtaccatt tgtccagggc ggatgaggcg tttatgccac atccgccagt
    16441 gtacgtcgat taatgcaact tcaaacgcgg acggataacg cggttaatac tccccaccag
    16501 catcattaat ccggttttaa agtaaccatg cagcgcaatc tgatgcattc ggtatagcga
    16561 gatatataca aagcgcgcaa ttcgtccttc aatcatcatt gagccgcgcg tcaagttacc
    16621 catcaggcta ccaacggtgg agaagttcga cagcgatacc agtgaaccat gatctttata
    16681 ctgataattt ttcagcggct taccgtttat ctgcgccaga atattgttca ttgcgcaagt
    16741 cgccatctgg tgtgcagcct gagcacgcgg tggaacaaag cccccttccg gacgcgtgca
    16801 tgacgcgcag tcgccaatag cgtaaatgtc tggatcgtgg gtggtttgca gcgtcggttc
    16861 caccaccagc tggttgatac ggttagtttc aagaccaccg atatctttca ggaagtctgg
    16921 cgctttgatc ccggctgccc acaccatcag atcagcctca atatattcac catctttagt
    16981 gtgcaggccg ccttcatcag cactggtgac catggtttgc gtcagcacgc gaacgccaag
    17041 tttcgttaac tcactatggg ccgcagcaga gatgcgtggc ggtaatgcag gcaaaatacg
    17101 ttctcccgct tctactagcg ttacgttcag ggcttcgttg gtcagacctt tataaccgta
    17161 gctgtgcagt tgcttgaccg cgttatgcaa ctcagcggag agttctacac ccgtcgcgcc
    17221 gccaccgaca atcgcaatgt tcactttgcc attcgcgccc aggttggcgg agtatttcag
    17281 gaacagattc agcatctcct ggtggaagcg acgcgcctgg tgcgggttat cgaggaaaat
    17341 gcagttctct ttgacgcctg gcgtattgaa atcgttagag gtgctaccca gcgccatgac
    17401 cagggtgtca taggcgattt tacgttccgg aaccagcagt tcacctttct cgtcgcgtag
    17461 ttctgcaata gtgattgttt tcgcttcacg atcaatatca atgacggaac ccaactggaa
    17521 ctggaaacca tgattgcgcg catgggccag atagctcaac gcatcgacgc cttcatcaag
    17581 cgagccagtc gccacttcgt gcagcagcgg tttccacagg tggctgtggt tacgatcgac
    17641 cagcgtaatt ttggcttttt tcttacgtcc cagcttatgc cccagctgtg ttgccatttc
    17701 cagcccacca gcaccgccgc cgacaatcac aatctttttc aatggcgtag tcaacgtgac
    17761 ccccttgaat ttattaacca attgttaatt aatggttatt aacatagctc ttaattaaca
    17821 acaggttaca gcaatgaaaa tgttcttcgc tattgagaat aacatgaatg gtgcattggt
    17881 cataccaaaa ttgatgttaa tcaagttttg ttgctgaaaa gataggcgac tttaccgggt
    17941 gagatcaaaa aatagagttt attgtttatt atttgagcga atttgtgaga gtgatataga
    18001 gtgaggggtg tatgcagggc gttcggtagg cctgataaga ggcgcatcag gcattgatgc
    18061 tcgactgtcg gatgcggcgt aaacgcctta tccgacttac agaattcgga gcgcatcagg
    18121 taccggcaag gttacctgat gcgacgaggg catttacccg agggttttga acgctttaat
    18181 gcgctgcaaa tgcggggaga tattcttgaa tttgtgcgtc tgctcttcgt cccagacaat
    18241 ctcgtaataa tgatgcaact cttcagatgt ccgctggctg ttaagcgctt catcattacg
    18301 cgacaaaatc accaggcaac gatcgcgatt cttctcgcgg aagttggtca cacacttagt
    18361 ggcaatatcg gcatactctt ccgggcgatc aatcttgcct tccatgttct cataagggaa
    18421 caaattaggg ttgaagatca cctggcggat atcgcaaaga aaaccaatcc gttccgccca
    18481 gtatccgccc agaccaacgc cgcaaatcag cggacgctcg tcgacgttca gttgcaacat
    18541 tttgtccact tctttaagca gatgctgcat atcatgtttc ggatgccgcg tactgtagct
    18601 tatcaagcgt acatccgggt caataaactg caattgtaag actttctcgt ggttacccgg
    18661 actgttagag tcaaaaccgt gtaaatagat aatcatcgct tcctcacata agccagggtt
    18721 agtgacctgc tttctcttcc tgccagcgtt catgtaactg attcagacgg gtgctgctcg
    18781 ccttccagcg agccgagtcc atcagttcct gtcgcgaaaa tgaaccttta tgatacaaac
    18841 gtgtaacacg ttctgccttg atcggtgaca gattatctaa cacgctgacg gcccctttac
    18901 gattattgca gaccaggatc atatcgcaac ctgcgtccag tgatgcctgc ccgcgttcgg
    18961 cataactgcc cataatcgcg gcaccttcca tcgataaatc gtcagagaaa attacaccgt
    19021 caaaacacag ttcctgacgc aaaacggttt tcagccagta agaagaaccg ctcgccggac
    19081 gcggatcaac atcactgtag atcacatgcg caggcataat ggcgtcgagt ttattttcgc
    19141 ggattaacgt gctgaaaacc gacatatctt tggcacgaat ttccgcttgc gggcgtgggt
    19201 cgcacggcgt ctctttgtgt gaatctgcag ttactgcacc gtgtcctggg aagtgtttcc
    19261 cggtcgtttt cattccggct tcatgcatcc catcaataaa ccgactagcg attgccaggg
    19321 ctttttctgg gtcggcatga taagaacgct cgccaatcgc cgcgctgata tgtcctacat
    19381 ccagcactgg cgcaaagctg atatcaatat ccatagcgat catttcgctg gccatcagcc
    19441 aacccgcctc ttgcgccagt ttgccgccct cttccattcc caacagcgca gcaaaggatt
    19501 gtgctgccgg taagcgggta aaaccttcgc gaaaacgctg cacgcgtcca ccttcctgat
    19561 ctaccgccac caccagatga ttgcgcgatg ctgcgcggat ctggcgcacc agttcacgta
    19621 actgggcagg atcatgatag ttacgcgtaa agagaatcag ccctcccacc agcggatgcg
    19681 ccagtatttc acgctcttcc gcgtccagtt cataaccttc gacatccaac attactggac
    19741 ccacactgct ctccttattg ttttattaat agctgccgcc aggtgtcatc ggccagcctg
    19801 ataaattgtt gatcgccggt ttgtcgccag cggtactcaa accaccctgc tttgagcatc
    19861 agcagccagg gaaaccatcg cctgacctga cgccataatt gcgccggata aatcttcgcg
    19921 cgagtggcat agtcattgac caattgccgg tgctgttcag tattttccac ccacaccgcc
    19981 gccagttcca gcgcggtatc accatctccg gcatactccc agtcgatgag ttttaaccct
    20041 gatgcgctat gcactaaatt tccggcgtgg acatccatat gcaatggact taagcgtaaa
    20101 ggtcgtggtt cccgcgcttt gcgcagacgt tttaacattc gcagccaacc cacagtccgc
    20161 cgcgccggat cgctttgctg ccagtacagt tccagtaacg gcaacagcgt tattcgccag
    20221 ccaaaacgtg gttgttgatg tagataatac agcaagcctg ccagttcgtt ggtatccggc
    20281 aaatacgttt ttaccgcgcc gggcagatag tcgactacca tccagtcacg gagatataaa
    20341 tgcggcttcg gtgcaatgct tgcgggtagt tgtgataaag cccgatactg gcgcaagaac
    20401 gcggactgcg gcgcatcagg atcgtgcggc tgacgcacaa caaaacgctg gccctgatgt
    20461 tcaatgagaa aactaccgcc actaagccca ctattaaacg tcgtgacggg atgaaactgc
    20521 gggaaaaagc gcgacagcaa ttcgtcgcgc gtgatgggat tattactgcg aaacggcacc
    20581 tttacctgac cagataattt cgcccgtctg caccagcatc agctgcattt gtagggtcgg
    20641 agcgttaacg ttgccagagg cgctggagta cagcacgtaa tgagcgccga cattgcgggc
    20701 aatgcctatg gctttactac gggtgcctaa actgtcctgc ggcgacaaac ctaactgttg
    20761 cttagccatc gacagttgct gggcggaaac cagggtaaat ttcccgttat tagccagcgc
    20821 atttcgcagc gtttcggtcg cttctgcggc attcagcgaa ccgttagtac ggttgttaac
    20881 gctatcaacc agcaggacgc tacccgcagt caccccgtca gccccaagca tcttactgac
    20941 tatcggctgc attgcgccat tccagtcata atggcgaata tgcggcgcag gcggtgccgt
    21001 tcgatcttcg tgctcaattg ggcctggctg ctgcgggatc gtcggcaccg agggcactgt
    21061 ggggacaggc tgttgtggct cggctggttg ttccggcgct ggtttcactt cttctaccgg
    21121 tgcaggttca cgttgcccca cacacccggc gagaaacatc gccagcgcgg taatcaaggc
    21181 gtagcgactc atttttgtca tcaagattca ccccttacaa atatagataa agtctgactt
    21241 tgtgcgcccc cagaaaattg gcgctgccgt acagcgttac cgccgaatgt gcgggaatgg
    21301 taacgctgcg tggcctttcc agaggatgca tctccagccc tctggcgtca taccagtaaa
    21361 aacgataatg aacggtaacg ggtttttgcc tttcgttata aagcgttgag gatgctgaag
    21421 gttgaatatc agacgtcgaa aggacgggct tttctgcact gatgcccgca gccaataaag
    21481 atgactccat caccagcgat tgctcatcat tcaccggaat ttccggatgt gaacgacagc
    21541 ccaccagcag taacaacacc agagacacca gcccaaagca tccttttctc atcgttacag
    21601 acctttatgc gccagcattg gtcccagcgg acggccaccc aacaagtgca tatggatgtg
    21661 gtaaacctct tgtccgccat ggcggttggt attcatgatc agacgatagc catcttcggc
    21721 aataccttct tgctcagcaa tttttgccac tacggtgatc atgcgtccca gcgcctgctc
    21781 atgctcagct gagacgtcgt tcacagtcgg aatgaggata ttcggaatga tcagaatatg
    21841 cgttggcgct tgcggcgaaa tatcgcgaaa cgccgttacc agatcatcct ggtagacgat
    21901 atcggagggg atctcacgac gaataatttt gctgaatata gtttcttctg ccacgacatt
    21961 ttcctttttc aaatagccat gcgttgcgcc atgctacgcc gcgccacact gcgagtatag
    22021 agtatggtcg agttaaacct gctctttcaa ccttaactca cgatttctta agcaaaaaat
    22081 cgttttgctg ccgtatatat cgccattatt cccatttctg cctgcgaatg cgctgaaaag
    22141 acattctctg tttggtttac acgacaaata tgaatgcgta tatttctcat ttgcatttac
    22201 aaacaaaatt attcgcacac taaatataat cgctcatcac cctcctccgg catgagcctg
    22261 acgacaacat aaaccatgag atttcaaatg ctttcaacac aatttaacag ggataatcaa
    22321 tatcaagcca tcaccaaacc gtcactactt gccggttgca tagcactggc actattacct
    22381 tctgccgctt ttgctgcatc agccactgaa gaaacggtga ttgttgaggg ttcagccaca
    22441 gctccagatg atgg
//
LOCUS       DAJTMC010000058        22360 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.058, whole genome shotgun sequence.
ACCESSION   DAJTMC010000058 DAJTMC010000000
VERSION     DAJTMC010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22360
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.058"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            <1..1298
                     /locus_tag="OO130_001939"
     CDS             <1..1298
                     /locus_tag="OO130_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HCR5365372.1"
                     /translation="WLELEGAKDLDDALSLLTILYHHVPSVTSMPVYLGQLDALLQPY
                     VRILTQDEIDVRIKRFWRYLDGTLPDAFMHANIGPSDSPITRAILRADAELKQVSPNL
                     TFIYDPEIPPDDLLLEVAKNICECSKPHIANGPVHDKIFTKGGYGIVSCYNSLPLAGG
                     GSTLVRLNLKAIAERSESLDDFFTRTLPHYCQQQIAIIDARCEFLYQQSHFFENSFLV
                     KEGLINPERFVPMFGMYGLAEAVNLLCEKEGIAARYGKEAAANEVGYRISAQLAEFVA
                     NTPVKYGWQKRAMLHAQSGISSDIGTTPGARLPYGDEPDPIIHLQTVAPHHAYYYSGI
                     SDILTLDETIKRNPQALVQLCLGAFKAGMREFTANVSGNDLVRVTGYMVRLSDLEKYR
                     AEGSRTNTTWLGEEATRNTRILERQPRVISHEQQMRFSQ"
     gene            1270..2133
                     /locus_tag="OO130_001940"
     CDS             1270..2133
                     /locus_tag="OO130_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HCR5365373.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVLWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVGSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQHLTGRDNQQIKRSICLLAERGKLAELRLLVIPDQ
                     VDYLHHIDELATFIKRLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(2347..3129)
                     /locus_tag="OO130_001941"
     CDS             complement(2347..3129)
                     /locus_tag="OO130_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR5365374.1"
                     /translation="MICRFIDTHCHFDFLPFSGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAEKYQPLYAALGLHPGMLEKHSDVSLEQLQQALERRPAKVVAVGEVGLDL
                     FGDDPQLERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLSRTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAVRVFDVLCELRPEPEDEIAEVLLNNTYALFSVSG"
     gene            complement(3126..4199)
                     /locus_tag="OO130_001942"
     CDS             complement(3126..4199)
                     /locus_tag="OO130_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HCR5365375.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGCYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVIDAPQANDPTFNNEDLA"
     gene            complement(4321..4482)
                     /locus_tag="OO130_001943"
     CDS             complement(4321..4482)
                     /locus_tag="OO130_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HCR5365376.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(4609..5214)
                     /gene="osmY"
                     /locus_tag="OO130_001944"
                     /pseudo
     CDS             complement(4609..5214)
                     /gene="osmY"
                     /locus_tag="OO130_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
     gene            complement(5607..7196)
                     /gene="prfC"
                     /locus_tag="OO130_001945"
     CDS             complement(5607..7196)
                     /gene="prfC"
                     /locus_tag="OO130_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HCR5365377.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     SRLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(7287..7964)
                     /gene="yjjG"
                     /locus_tag="OO130_001946"
     CDS             complement(7287..7964)
                     /gene="yjjG"
                     /locus_tag="OO130_001946"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HCR5365378.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGIAKPNKKIFDYAL
                     ELAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHHLEQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(7979..8425)
                     /gene="rimI"
                     /locus_tag="OO130_001947"
     CDS             complement(7979..8425)
                     /gene="rimI"
                     /locus_tag="OO130_001947"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HCR5365379.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKNFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIVVDPDYQRQGLGRVLLKHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(8394..8807)
                     /gene="holD"
                     /locus_tag="OO130_001948"
     CDS             complement(8394..8807)
                     /gene="holD"
                     /locus_tag="OO130_001948"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HCR5365380.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            8910..9941
                     /gene="rsmC"
                     /locus_tag="OO130_001949"
     CDS             8910..9941
                     /gene="rsmC"
                     /locus_tag="OO130_001949"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HCR5365381.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVATVDDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANCVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            10209..10295
                     /locus_tag="OO130_001950"
     tRNA            10209..10295
                     /locus_tag="OO130_001950"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10243..10245,aa:Leu,seq:cag)
     gene            10324..10410
                     /locus_tag="OO130_001951"
     tRNA            10324..10410
                     /locus_tag="OO130_001951"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10358..10360,aa:Leu,seq:cag)
     gene            10446..10532
                     /locus_tag="OO130_001952"
     tRNA            10446..10532
                     /locus_tag="OO130_001952"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10480..10482,aa:Leu,seq:cag)
     gene            complement(10571..10807)
                     /locus_tag="OO130_001953"
     CDS             complement(10571..10807)
                     /locus_tag="OO130_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HCR5365382.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYVLLPSCLALIAAIMLMIMNLNQG"
     gene            10948..11736
                     /gene="fhuF"
                     /locus_tag="OO130_001954"
     CDS             10948..11736
                     /gene="fhuF"
                     /locus_tag="OO130_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HCR5365383.1"
                     /translation="MAYRSAPLYEDIIWRTHLQPQDAGLAQAVRATIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            complement(11774..12450)
                     /gene="bglJ"
                     /locus_tag="OO130_001955"
                     /pseudo
     CDS             complement(11774..12450)
                     /gene="bglJ"
                     /locus_tag="OO130_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
     gene            complement(12408..13133)
                     /locus_tag="OO130_001956"
     CDS             complement(12408..13133)
                     /locus_tag="OO130_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR5365384.1"
                     /translation="MLPGCCKNGIVISKIPVMQAGLKEVMRTHFPEYEIISSASAEDL
                     TLLQLRRSGLVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAVELLM
                     CPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMNETHDFSYRSVILTLSERK
                     VLRLLGKGWGINQIVSLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGARELN
                     LPSVYGDAAEWNTAELRREMSHS"
     gene            13752..14522
                     /locus_tag="OO130_001957"
     CDS             13752..14522
                     /locus_tag="OO130_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HCR5365385.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNKGGWDGAIITFFASTAAMYIRQLLAQ
                     HHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            14513..14986
                     /locus_tag="OO130_001958"
     CDS             14513..14986
                     /locus_tag="OO130_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HCR5365386.1"
                     /translation="MGVIEFLFALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGAI
                     GHGSRMILMTRGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            15094..15633
                     /gene="dnaT"
                     /locus_tag="OO130_001959"
     CDS             15094..15633
                     /gene="dnaT"
                     /locus_tag="OO130_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HCR5365387.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            15636..16373
                     /gene="dnaC"
                     /locus_tag="OO130_001960"
     CDS             15636..16373
                     /gene="dnaC"
                     /locus_tag="OO130_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HCR5365388.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            16422..16916
                     /locus_tag="OO130_001961"
     CDS             16422..16916
                     /locus_tag="OO130_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HCR5365389.1"
                     /translation="MMKTVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            17170..19461
                     /gene="opgB"
                     /locus_tag="OO130_001962"
     CDS             17170..19461
                     /gene="opgB"
                     /locus_tag="OO130_001962"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HCR5365390.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRHRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIAAFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWQGKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(19600..20622)
                     /gene="lgoD"
                     /locus_tag="OO130_001963"
     CDS             complement(19600..20622)
                     /gene="lgoD"
                     /locus_tag="OO130_001963"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HCR5365391.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVRLGKNIADLKNGLQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPLLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            20761..21675
                     /locus_tag="OO130_001964"
     CDS             20761..21675
                     /locus_tag="OO130_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR5365392.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSHLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTLINQR
                     QLRPGETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            21890..>22360
                     /locus_tag="OO130_001965"
     CDS             21890..>22360
                     /locus_tag="OO130_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5365393.1"
                     /translation="MEKENITIDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHNFTQFVLVRIGMGIGEAPMNPCGVKVIND"
BASE COUNT         5524 a         5847 c         5532 g         5457 t
ORIGIN      
        1 aatggctgga gctggaaggc gcgaaagatc ttgatgacgc actctctctg ctgaccattc
       61 tttaccacca cgtaccgtcg gtcacatcga tgccggtcta cctagggcaa ctggatgcgt
      121 tgttgcaacc gtatgttaga attctaacac aagacgagat cgatgttcga ataaaacgtt
      181 tctggcgtta cctcgacgga accctgccag acgcctttat gcacgccaat atcggcccgt
      241 ctgattcgcc cattacccgc gcgatcttac gtgcagacgc agagttgaag caggtttcac
      301 cgaacctgac ctttatctac gatcctgaaa tcccccctga tgacctgctg ctggaagtgg
      361 cgaagaacat ctgtgaatgt agcaaaccgc acatcgccaa cggtccggtg catgataaaa
      421 ttttcacaaa agggggctac gggattgtga gctgttacaa ctcactgccg ctggcgggtg
      481 gtggcagcac gctggtacgc cttaacctga aagccattgc cgagcgcagc gaatcgctgg
      541 atgacttctt tacgcgcact ctaccgcact actgccagca gcagatcgcc atcatcgatg
      601 cgcggtgtga attcctctat caacaatcac acttctttga gaatagcttc ctggtgaaag
      661 aagggctgat taaccctgaa cgttttgtgc caatgtttgg catgtatggg ctggcggaag
      721 cggttaactt gctgtgtgag aaagaaggga ttgccgcgcg ctacggtaaa gaagccgccg
      781 caaatgaagt gggttatcgc atcagcgcgc aactggcgga gtttgtcgcc aatacccccg
      841 tgaaatatgg ctggcaaaaa cgcgccatgt tacacgcaca gtcggggatc agttccgata
      901 tcggcaccac gccgggcgcg cgtttgccgt atggcgatga gccagatccg atcatccatc
      961 tgcaaactgt cgccccgcat catgcttatt attattccgg catcagcgac attctgacgc
     1021 tcgacgaaac catcaaacgt aatccgcagg cgctggtaca gctttgcctc ggtgccttta
     1081 aagccggaat gcgtgaattt accgccaatg tcagcggtaa cgatctggtt cgcgttaccg
     1141 gttatatggt gcgtttgtcg gatttagaaa aatatcgcgc cgaaggttca cgcaccaaca
     1201 ccacctggct gggcgaagaa gccacacgca acactcgtat tctggaacgc cagccgcgcg
     1261 tgataagcca tgaacagcag atgcgcttta gtcagtaaga ttatcccctt ctcctgcgtt
     1321 gacgggccag gcagtcgtct ggctctgttt ctgcagggct gcaatctgcg ctgcaaaaac
     1381 tgtcacaatc cgtggacgat gggacgctgc aatgattgcg gagagtgcgt gccacagtgt
     1441 ccgcatcagg cgttgcagat tgttgacggc aaagtgctgt ggaacgctgt ggtctgcgag
     1501 cagtgtgata cctgcctgaa gatgtgtccg caacatgcca cgccaatggc gcaatccatg
     1561 agcgtggacg aagtgcttag ccatgtccgc aaagcggtgc tgtttattga agggataacg
     1621 gtaagcggcg gcgaagccac gacccaactg ccgtttgtag tggcgctgtt tactgctatc
     1681 aaaaacgatc cgcaactgcg ccacctcacc tgcctggtgg gcagtaacgg catgttgagc
     1741 gaaaccggct gggaaaaatt actcccggtg tgtgacggcg caatgctcga tctcaaagcg
     1801 tgggggagcg aatgtcatca acacttaacc ggacgcgata atcagcagat taagcgcagc
     1861 atctgtttgc ttgcagagcg cggcaagctg gcggaactgc gtttgctggt aattccagac
     1921 caggtggatt atttgcatca catcgatgag ctggcgacgt ttatcaagag acttggcgat
     1981 gttccggttc gcctgaatgc gtttcatgcc cacggcgtgt atggcgaggc gcaaagctgg
     2041 gcgagcgcca cgccggaaga cgttgagccg ttggctgatg cgttaaaggt gcgcggggtg
     2101 agccggttga tatttccggc gctctatttg tgacatgccg gatacggcct acggttcggc
     2161 acagacttgt aggcatgata agacgcgaca agcgtcgcat caggcattgt gcgccaactg
     2221 ccggatgcgg cgtgaacgcc ttatccggcc tacggttcgg cacaaacctg taggcatgat
     2281 aagacgcgac aagcgtcgca tcaggcatta tgcgccgact gccggatact gcctacgaag
     2341 ggtaaactac ccactaacgc taaacaacgc atacgtatta ttaagcaaca cttcggcaat
     2401 ctcatcttct ggttccgggc gcaactcgca aagcacgtca aacacccgca cagcctgttc
     2461 cggtcggtta ggctgcccct gaaagccgtt gagcggcata tccggcgcgt cggtttccag
     2521 caacaacgat gccagcggta attttgcgat gacatcgcgg gttttactgg cgcgtggata
     2581 ggtgatagta ccgcctacgc cgattttgta gcccagctgt acaaaccgct cggcctgttg
     2641 caggctgccg gaaaaaccgt gaaccacgcc agtgcgcgat aaatcgtggc gtttaagatg
     2701 catcgccagt ttgtcgtgtg tgcgccgtga atgcaggatc accggcagat cgtagcgttt
     2761 cgccagtttc agctgttcgt cgagtaacca ctgctgcctc tcaagttgcg gatcgtcgcc
     2821 gaagagatcc agaccgacct cccccactgc caccaccttc gccggacgcc tttccagcgc
     2881 ctgctgtagc tgctcaagag acacatcgct atgtttttcc aacatacctg gatgcaagcc
     2941 caatgcggca tacagcggtt gatatttttc cgctaacgcc tgcacacggg caaaattttc
     3001 cgcctctgtc gccggaacaa tgatcttgcc tacgcccgct tgtgccgcgc gttgcaggct
     3061 ggcctcttca tcgccactaa acggcaggaa atcaaaatgg cagtgggtgt cgataaaacg
     3121 gcaaatcaag ccagatcctc attgttaaat gtcgggtcgt tcgcctgcgg agcgtcaatc
     3181 acttcggcaa ccagcgtatc gttggcgact ggcgcaggcg gaataacgat cgattccggc
     3241 gtcaccactg gcaccagatg gcgggtaagc ggcgcttttt cagttaatag cttgccaacc
     3301 gtggcgagga aataacaacc gcataaacgc ccaagtttat agtcttcacg cagcgccgga
     3361 atccgactgc caagcgcgat actatgtaat ggcttcggcg gataaatttc gaatatccgc
     3421 agcttgcccg gtggtttctc aataaattgc tgaatgtcac gatagctggt ttcatgatgc
     3481 tgcaccagat tgaccagcgg ctgcaggcta ctgtcaccca gccagcgttc catgcgtttg
     3541 aaccactgcg gcgtgtagta catttgtgac ggcacagtgc gaatgacgac caacgtttta
     3601 gcgccctgcc ttgccgcctc tttaaccgga atcgcatcac tgatcccgcc atccaggtag
     3661 ttaatgcctt ccagcgacac tccgctacga taaaagccag gtatcgcact ggaggcgcga
     3721 atcacatcca gccagttttg tttggttggt aaaaagtaat taggcgcgta gtcatcctga
     3781 cgacaggcgc acatataaaa cgatttgccg ctgtcaaaca accgcgcggc ggtatccatt
     3841 tgcaacggca tctggcttgc ggtggcctcc accagccagt cgagatcgat aagatttcct
     3901 ccacggacaa agcgcaatgg atcgaaaaat tcgcgttttg tggtatagcg catgatgact
     3961 ttacgcgcgt aaccgggctg attgcagata aacgccgaga ggttctgcgc cccggcagat
     4021 gtgccgagat aaagatcgaa aggattaaac tgcgcgcgca taaactcatc cagcacgcca
     4081 gccgtgaaaa ttccacgctg tccgccgcct tcgcacacca gagctattcg tccaggctgg
     4141 aacggcctta gcgacaacgg cgcaatatta ccaagcgtta caggtattcg ctgccccacc
     4201 tctgccttcc tgtttttgtt gtgaaagaat gacaaagtaa cgcaatttat tattcgctaa
     4261 aaccgcaaaa gccagtccgc tggactggct ttggataaaa ttttcttaat ggtctgaaat
     4321 ctagggtcgt tttcggccca tgaacaaact caccaggaac agaataatcc cgacgacaaa
     4381 gacaatttta gctgcgcctg cagcggtacc ggccaaacca ccaaacccaa gtgcggcggc
     4441 gattaacgcg ataaccagaa atatgatgcc ccaacgaaac ataagcgtct cctttaccat
     4501 agttaatgtc accgcttgat atgagcgtga aaatcactca atgcgatatt tttagtgtgg
     4561 tgcacatcgc gcctcccgac aaagttcggg aggacgaatt acgacgaatt acttagtttt
     4621 cagatcattt ttaacgcttt tcacaccatc taccgctttg gcgatacttt cagcacggtc
     4681 actttgtgcc tgagaatcta cggtaccgga gagctgaacc acgccgtcgg tggtttcaac
     4741 tttcacatga cgggaaggga cgatatcgtc cgccagcagt ttggctttga tttcactggt
     4801 ggtggcggtg tcacccgcgt agcccttcac cgagccttct ttagcgtcgc gaacgtgcag
     4861 tttgtcgctg acagaggtca ccccttcaac gcctttcgcc actttcactg cctcttcggc
     4921 ctgagcctag ctttcaacga aaccgctcag ggtcacgact ttttgctcgg ttttgacaga
     4981 gatatcggtg ctcttgatgt tgtcatgatc caccagcgcc gccttcactt tcgcggtgat
     5041 ggcgctgtca tccatgaaat taccgacttt attcatagag ctatcgactt tttgccctgc
     5101 gctttcattg gtagtctgtg cgttgttttc cgcgtaggca gagccggtcg cgacggcaga
     5161 ggtcaacatt acagccagca gagttttcga aatcttcagt cttgtcatag tcatcgattt
     5221 attcctgtat gtttgctcgt aatttgagcc tggcaacacg aggttgcatt gctgaatagg
     5281 gagagacttc accctctaca gaagtcaatg gccgccatca caaaagcgat gggtgatgaa
     5341 taacgaccat tacagcctct gaatcagtta ttaatatcgg tagaatgaca atcgacggct
     5401 ttagatactg atatctacgc attgaacggt atttaacgcc gtcagaaatg tcattacttt
     5461 gttaaatata gatcacaatt ttgaaaccgc tcgtgatatc agcgaaaaca taagcaaaag
     5521 tgaatgtttt aagaacattc cgtaagcggc taataaggaa gggaaattga cagggcgcag
     5581 cggctaccgc gccctggagg caagaattaa tgctcgcggg tctggtggaa ctgaacgtcg
     5641 ggataacgtt cctgcgccag gcgcaggttg accatgctgg tagcgatgta agcgaggtta
     5701 tcgccgccat caagcgccag ttggctttcg ttcttacgct tgaactcttc gaatttcttc
     5761 gcgtccgcac attctaccca gcgggcagtg gcaacgttga ctgattcata tactgcttca
     5821 acgttgtatt cgctcttcag gcgcgatacc accacatcaa actgcagcac accaacagca
     5881 ccaacgatca aatcgttgtt ggagatcgga cggaacacct gcaccgcgcc ctcttcggaa
     5941 agctgtacca gccctttgag cagctgtttt tgcttcagcg gatctttcag gcggatacga
     6001 cggaacagtt ctggtgcgaa gttcggaata ccggtgaact tcatcatctc accctgggta
     6061 aaggtgtcgc cgatctgaat agtgccgtgg ttgtgcaggc cgaggatatc gcccggatac
     6121 gcttcttcaa cgtgcgaacg gtcgcccgcc ataaaggtca gcgcgtcgga gataaccaca
     6181 tctttcgcag tacgcacctg gcgcagcttc atgccttttt catatttacc ggatactacg
     6241 cgcataaacg ccacgcggtc gcgatgtttc gggtccatat tggcctgaat tttaaatacg
     6301 aagccggtaa atttgtcttc gctcgcctct acggtacggg tatcagtctg acgcggcatc
     6361 ggcgcagggg cccactccac caggccatcc agcatatgat caacgccgaa gttacccagc
     6421 gcagtaccga agaataccgg agtgatttcg cccgcaagga acagttcttt gtcgaactcg
     6481 ttagacgcgc ctttcaccag ttccagttcg tcacgtagct gctgtgccag atcttcacca
     6541 accgcagcgt cgagatccgg gttattcagc cctttaacaa tacggacttc ctgaatcgtg
     6601 tggcctttac cgctctgata aagataggtt tcgtctttat aaaggtggta aacgccttta
     6661 aacagcttgc cgcagccaat cggccaggtg atcggtgcgc agccgatttt cagctcgttc
     6721 tcaacttcat cgagcagttc catcgggtcg cggatatcac ggtcaagttt gttcataaag
     6781 gtgaggatcg gcgtgtcgcg cagacgggta acttccatca gcttacgggt acgatcttca
     6841 acaccttttg cagcgtcgat aaccatcagg cagcagtcca ccgctgtcag ggtacgatag
     6901 gtatcttccg agaagtcttc gtgccccggg gtgtctagca ggttaaccag gcaatcgtga
     6961 tacggaaact gcatcacaga cgtagtaatg gagatcccac gctgcttttc catctccatc
     7021 cagtccgact tagcgtgctg gttggaacca cggcctttta ctgtaccagc ggtctgaatg
     7081 gcctgtccga acagcagcac cttctcggtg atggtagttt taccggcgtc cgggtgagaa
     7141 ataatggcaa aagtgcggcg cttcgccacc tcttgcaaat aaggagacaa cgtcataatt
     7201 ctttcttctt gagtaagcgg catcgcgccg cgcttgttga aaacgaaaaa ttgcggctat
     7261 tttacccatc aacggggggg aggcaatcag tgtttacaca ggagctgctc cagttcgtgc
     7321 aacgatgaaa cggtccaggt gggcgcgatg ccttctggtt gctcgagatg gtgcgcattc
     7381 agccagcaag tcgcaagccc ggcgttgatg ccaccgagaa tatcggactc ggcagtgtcg
     7441 ccaaccatca gcacgcgtga acggtcagga ttgcccgcca gttccagcgc ataatcgaaa
     7501 attttcttat tcggtttggc aatgccaact tcttcggaaa tcaccagcaa atcaaagtaa
     7561 tcacgcaggc ccgtccgttc cagacgcact tgctgcaagg cgctaaaacc gttggtgatg
     7621 atgccgattt tagcgttgcc acgaatggcg ttaagcagag aaaccgcgcc cggcagcggc
     7681 gtgcagattt ccgccatcgc attaataaag gcatcgttaa gcaagcctgc ttcaactttc
     7741 agccgttcgg cccagctctc gaaacgcccg tgctgaagct gtaatgaagt gatcgcgccg
     7801 ttttgataat ccacccacag tggcttgtta acggcctgat agtcctgaaa atcttcagcg
     7861 gtaaaggtga cgctgtaatc aagaaacatc cgctgcaggc cggtgaatga gtcaaaggta
     7921 aacagcgttt catcggcatc aaagaaaatc cagtcccact tcattattcc accttgtctt
     7981 acatactgat tggcaacgcc atgatgatgg cgtcttcgcg accgtccgtg gtggggtagt
     8041 aattgcggcg aatcgtcgcc tcgttaaagc ctaaactttc gtacagggca atggcggcag
     8101 cgtttgaagc acggacttcc agccatagtg tcgccacgcc gcgtttttcc agttcgtcga
     8161 tcagatgttt cagcagcacc cttcccaatc cctgacgctg atagtcagga tcgaccacaa
     8221 tattgaacaa tgtagcttca tccagcacca cttgcgtaat cgcaaacgcc gccattttgc
     8281 cgttttgcgt taactgaaag ttgagataac gctcgccctg gttgctggca aagttttttt
     8341 cactccacgg aaaggcgtgg gcgcgttgtt caatgtggta agccgctggt aaatcagtcg
     8401 tttcgaggga agaaatcgtg ttcatatgtg caaatctgtt gccataacgc ggcgcgtgcc
     8461 gttgggtttg cccgtaattc ggtgagcgcc ggtgatgcca cctgagcgcc ttccagtgat
     8521 agcggttcgt cagtacccaa ccgccagctg ttgcagcgac tgccttgcgg cagcatcgcg
     8581 attttttctg gcgtcagttg cagcacttga tcggggctga cggttaatgc gcgcagaaca
     8641 tcgctcacta aaggatcggt cagggcggga agatcgtttg ccaccatcac cagacggacg
     8701 tgtgccggga tcgcaatggc aatctccccc tgcaacgcgc cagggcgacg cagcgaccac
     8761 tgggtaatgc ccagttgctg taactgccag tctcgtcggg atgtcatagc gaatcgctcc
     8821 tgttgtcagg ggcgcaaata tagcaaattc gtcgataccg cgccaacata tggctataat
     8881 cgccgccagt atcaattgag gagcattcca tgtctgcatt taccccggca agtgaagtct
     8941 tgctgcgtca cagtgatgat ttcgaacaaa gccgtattct gtttgccgga gacttacagg
     9001 atgacctgcc cgcgcgttta gataccgcgg ccagccgtgc tcatacccag caattccacc
     9061 actggcaggt attaagccgc cagatggggg ataacgcccg tttcagtctg gtcgccacgg
     9121 tggatgacgt cgcagattgc gatacgctga tttactactg gccgaagaac aaaccggaag
     9181 cccagttcca gttgatgaat ttactttctc tgctgccagt ggggacagat atttttgtcg
     9241 ttggcgagaa ccgcagcggc gtgcgcagcg ccgagcagat gctggcagat tatgcgccgt
     9301 tgaataaagt cgacagcgct cgtcgctgtg gcctctattt tggtcgtctg gaaaaacagc
     9361 cggtatttga tgccgataaa ttctggggcg aatacagcgt cgatggcctg acggtcaaaa
     9421 cgctgcctgg cgtgtttagc cgcgacggtc tggatgtcgg tagccagttg ctgctctcga
     9481 cgttaactcc gcacacgaaa ggtaaagtgc tggatgtcgg ctgtggcgcg ggggtgcttt
     9541 cagttgcctt tgcgcgccat tcgccgaaaa ttcgtctcac cttgtgcgat gtctctgcgc
     9601 cagcggtaga agccagccgc gcaacacttg cggccaactg tgttgaaggt gaagtctttg
     9661 ccagcaacgt cttttccgag gtgaaaggtc gttttgatat gatcatctcc aacccgccgt
     9721 tccacgatgg gatgcaaacc agcctggatg cggcgcaaac gctgattcgc ggcgcggtgc
     9781 gtcatcttaa tagtggcggc gagctgcgaa ttgtagcgaa cgccttcctg ccttatccgg
     9841 acgtgctgga tgaaacattt ggcttccatg aagtgattgc gcaaaccggg cgtttcaagg
     9901 tatatcgcgc cattatgacc cgccaggcga aaaaaggtta agtatcccgc cgcaagcctt
     9961 gctggatgcg gcgtaaacgc cttatccgac ctacaaaacc tgcacgttag cccttcgtag
    10021 gccagataag acgcgccagc gtcgcatctg gcatctgccc ccgcatgcct accaaacctg
    10081 cacgctagtt tcctgatgga catttttcca gcaattacac ctccggcgat aattaactat
    10141 tgacgaaaag ctgaaaacca ctagaatgcg cctccgtggt agcaattctt tttaagaatt
    10201 gatggtatgc gaaggtggcg gaattggtag acgcgctagc ttcaggtgtt agtgtcctta
    10261 cggacgtggg ggttcaagtc ccccccctcg caccaaaaac cacgttgata ttgctcgcac
    10321 tgggcgaagg tggcggaatt ggtagacgcg ctagcttcag gtgttagtgt tcttacggac
    10381 gtgggggttc aagtcccccc cctcgcacca aacgaggcga tatcaaaaaa aagtaagatg
    10441 attgtgcgaa ggtggcggaa ttggtagacg cgctagcttc aggtgttagt gtccttacgg
    10501 acgtgggggt tcaagccccc cccctcgcac caattatctt tacttccttt cttgtttctt
    10561 ccttgatttt tcatccctga ttcaaattca ttatcatcag catgatggca gcaataagcg
    10621 ccaggcacga tggtagcaaa acgtaatgcc gtaactgttt gtgatacagc atcaccggcg
    10681 tcaatactaa tcccatcaga atgacaatcc gccactgatc cggcgataaa tccgcataca
    10741 tcagccctgc aatcagcaat cccggcagca acactcccca gccactgccc agcgtacgtt
    10801 gcaacatggt ttcatctctt tcattgataa tgataactaa tatcatatga taatttttat
    10861 catttgcaag ccagagaaat cccttgctat cgggtaaacc tatcgctagg attagcaatc
    10921 attatcattt agattactat cccgattatg gcctatcgtt ccgcaccgct ctatgaagat
    10981 atcatctggc gaacgcatct ccagccgcag gatgctgggc ttgcacaagc cgtacgtgcg
    11041 acgatcgcca aacatcgtga acatttgctc gagtttatcc gcctggatga acccgctccg
    11101 cttaacgcca tgacgctggc gcaatggtca tcaccgaatg cgctaagttc tctactcgcg
    11161 gtctattccg atcatatcta tcgcaaccaa ccaacgatga tccgcgagaa caaaccgctg
    11221 atctcactat gggcacaatg gtatatcggc ctgatggtgc caccattaat gctggcgcta
    11281 ctgacgcagg aaaaggcatt agatgtgtcg ccggaacatt tccatgctga gtttcacgaa
    11341 accggacgcg tcgcctgttt ctgggtcgat gtgtgtgaag ataaaaacgc aacaccacat
    11401 tcgccgcagc agcgaatgga aacgttaatc agccaggcgc tggttccggt tgtgcaggca
    11461 ctagaagcga ccggagagat caacggcaaa cttatctgga gtaataccgg ttatttgatc
    11521 aactggtatc tcactgagat gaagcaactg ctcggcgagg cgacggttga atcgctgcgc
    11581 catgccctct tttttgagaa aacgctcacg aacggtgaag ataatccact ctggcgtacc
    11641 gtcgtgctgc gcgatggcct gctggtgcgc cgaacctgct gccagcgtta tcgcttaccg
    11701 gatgtgcagc aatgtggcga ttgtacgctg aaatgaaagc actgccgggg aagtaaaccc
    11761 ggcatcatgc ggattaatag ggatgcaaca cattacttgc ttcgcaatgc cgcatacata
    11821 acagaatatc tgcggcctcc aacacccctg catcagaact gacgcccagt ttcgacatca
    11881 cattaaattt atgtgcacgg atcgttttga tattgcgttt aagctgctcg gcaatttgtg
    11941 tcattgagta gccacgcgac ataaagcgca atatttctct ctccgtcggg cttagcgtcc
    12001 ggctttggtt aatgtaccac tgattgttca gtcggtcggt cgcctgacgt acaccattta
    12061 atgacaaaaa gagttcctga tgaaaaatct ccagcgttga cgctttactt aatacaccgt
    12121 ccagcggtga tggcgacaat gaaccaatca gccgagcttc aatatcatca tccgcaataa
    12181 ctaaacgccg ggtacgcgga aacttaatcg ccagttcagt caggcaagat aatccctctc
    12241 tgcgctcact tctcatggca gaaaaagaaa aatgaccgcc gcaaatgaga tccgcgacat
    12301 ggcatcctga aatgactcct gactggtata ggtatggagc ttataagggt tacccgcaaa
    12361 ctttctgaat aaactctcaa taccgatact gctcatgacg catttttcta tgagtgcgac
    12421 atttctcttc ttaattcggc tgtgttccat tctgcggcat ctccataaac agaaggcaag
    12481 ttaagttctc ttgcaccctg cgcgctattt atccatgcat acatttcagc gttgctgtga
    12541 attgccagtc gacgcatcgc actgtttttt tgggcgctga tagttttatt acttttctta
    12601 agcaacgaaa ctatctggtt gatgccccat cctttaccta atagccgcag taccttgcgc
    12661 tctgaaagag tgagaatgac ggagcgatag ctaaaatcat gagtttcatt cattgccgga
    12721 gaggtcagca tggcgctgat acgctctgcg tgcgtattgc cggaacgtac ggtcttgacc
    12781 agattctcaa tgggttcaac atcagacaat aacgtcgccg tagggcacat gagcaattct
    12841 actgcctggg aataccagga gcgtgacacc atgaaaaccc agtgaatttc ccgatattgt
    12901 gagattaaag aataataatg ttcacaaaca gaacgtggat cttcactttc accggctaaa
    12961 tcagcaatga ctaatccgga acgacgtaat tgtaataagg taaggtcctc cgcagaggcg
    13021 ctggatatta tttcatattc agggaagtga gtcctcatga cctcttttaa ccctgcttgc
    13081 ataacaggta ttttactgat aacaattcca tttttgcagc atcctggcaa catatttcct
    13141 ccacatccac tatctaaaaa tcatgatggt ttattcagga tattcattcc ttaaatgttt
    13201 ccagcaaatt aatagattaa gcgaataaat tatccttagc attcactttc atatcagcat
    13261 actcctgctg cacaacaact ccactgcatg tgtcagaagg ttagcatcaa atgataacct
    13321 agtgaaatag cctggagaca tttaaaatat ctacattaag ttgttaggaa tacttaatga
    13381 aaaacagaaa tcacattcat ttaatttata attaaatgat atcccttccc gcagaatgaa
    13441 aagtcacaca accaatattt attcaatgaa atctgatggt tttaacttgt taacattaaa
    13501 aaacgtgccc ggttatctat tatcctgatt cgtaaaggcc aaacaataaa ttcattatag
    13561 tgataatatt catgtgatag tttgtttgcg gcgagagata attcgctttt tatcaccgag
    13621 cttgtcccga taaccaggat taatcagcgc aattttgatc ttaacctacc gcaaaacaac
    13681 cgtgcttcat gatacaaatg gattctcatt atctgaacaa tacgcagcac gtctatgaca
    13741 aagggcgagt tatgcaaact gagcaacagc gagccgtaac acggctttgt atccagtgtg
    13801 gattatttct tttgcaacat ggtgcggaaa gcgcgttggt tgatgagctt tcctcacgac
    13861 tgggtcgggc gttggggatg gacagcgtcg aaagttctat ctcttcgaac gccatagtgc
    13921 tgacaaccat taaagatggg caatgcctga catcgacacg taaaaatcac gatcgcggca
    13981 ttaatatgca tgtggtgact gaagtccagc acattgtgat tctggcggag caccatctgc
    14041 tggattacaa aggcgtagag aaacgattta gccaaattca gccattacgt tacccaagat
    14101 ggctggtagc cttaatggtt ggcctttctt gcgcctgttt ctgcaaactc aataaaggtg
    14161 gctgggatgg tgccatcatc accttctttg ccagtacggc cgcgatgtat atccgccagc
    14221 tgctggcaca acatcatctt catccgcaga tcaacttttg cctcaccgct ttcgccgcca
    14281 ccaccatttc cggattgctt ttgcaacttc ccactttcag caatacaccc actatcgcga
    14341 tggccgccag cgttctgctg ctggtgccgg gatttccgtt gattaatgcc gtcgccgata
    14401 tgtttaaagg ccacatcaat accggactgg cacgctgggc gatcgccagt ctgctgacac
    14461 tggctacctg cgtcggcgtg gtgatggcac tgacgatttg ggggctacgc ggatgggtgt
    14521 gatcgagttt ctgtttgcat tggcgcagga tatgatcctc gccgccattc cagcggtcgg
    14581 ctttgcgatg gtgttcaacg ttcccgttcg ggcattacgc tggtgtgcgc tgcttggcgc
    14641 gattggtcat ggttcacgaa tgatcttaat gaccagaggg ctgaatattg agtggtcaac
    14701 ctttatggcc tccatgctgg tcggcaccat tggtattcaa tggtcgcgct ggtatctggc
    14761 gcatccgaaa gtgtttaccg tggcggctgt tatccctatg ttcccgggca tatcggctta
    14821 taccgcaatg atttcggcgg taaaaatcag ccagttaggt tacagcgaac cgttgatgat
    14881 taccctgtta accaactttc ttacagcttc atcgattgtt ggtgcgttat ccatcggtct
    14941 ttccattcct ggattatggt tgtaccgcaa gcgccctcgc gtataaaatt gcctcgctta
    15001 aagcacacgg atgagagaca gcctcctctc ctccgtgtgt tactataaaa gttatctccc
    15061 ttctcgttca tcgttccata tttgagaaac agtatgtctt ccagagtttt gaccccggac
    15121 gtcgttggta ttgacgccct ggtacacgat caccaaaccg ttctggcaaa agctgaaggc
    15181 ggtgtggttg ccgtatttgc taacaatgcc ccggcgtttt atgccgtcac gcctgcacgc
    15241 ctggctgaac tgctggcgct ggaagaaaag ctggcgcgtc cgggaagcga tgtcgctctg
    15301 gacgatcaac tctatcagga accgcaagcc gctcccgttg ctgtacccat ggggaaattc
    15361 gccatgtatc cggactggca acccgatgcc gattttatcc gcctggcggc gctatggggt
    15421 gtggcactaa gagagccggt gaccaccgaa gaactggcct cattcattgc ctactggcag
    15481 gcggaaggta aagtctttca ccatgtgcag tggcaacaaa aactggcgcg cagcctgcaa
    15541 atcggtcgtg ccagcaacgg cggactgccg aaacgagatg tgaatacggt cagcgaacct
    15601 gacagtcaaa ttccaccagg attcagaggg taacgatgaa aaacgttggc gacctgatgc
    15661 aacgcctgca aaaaatgatg cctgcccata tcaaacctgc attcaaaacg ggtgaagaac
    15721 tactggcctg gcagaaagaa caaggagcga tccgctccgc cgctctcgaa cgtgaaaatc
    15781 gggcgatgaa aatgcagcgc acctttaacc gctccggtat tcggccactg catcagaact
    15841 gctcctttga gaactatcgc gttgagtgtg aagggcagat gaatgcgtta agcaaagcgc
    15901 gccagtacgt cgaagagttt gacggcaaca tcgccagctt catcttttct ggtaagccag
    15961 gaaccggcaa aaaccatctg gcggcggcaa tctgcaacga gctgctgcta cgcggtaaat
    16021 ccgtattgat catcaccgtg gccgatatta tgtcggcgat gaaagatacc ttcaggaata
    16081 gcggtaccag tgaagaacaa ctgcttaacg atctgagcaa cgttgatctg ctggtgatcg
    16141 atgagatcgg cgtgcagacc gaatcgaaat acgaaaaagt gatcatcaac cagatcgtcg
    16201 atcgccgctc ttcttccaaa cgcccaaccg ggatgctgac caacagtaat atggaagaga
    16261 tgaccaagtt gctgggcgaa cgcgtgatgg accgtatgcg cctgggtaac agtttgtggg
    16321 tgatcttcaa ctgggatagc taccgtagcc gggtaacagg taaagagtat taatccccgt
    16381 ttctggcggt atactgcgac acaattccac cttgagtaat gatgatgaaa actgttaaac
    16441 atcttctgtg ctgtgccatt gccgccagcg cattaatttc caccggggtg catgctgcgt
    16501 cctggaaaga tgcgctctcc agcgccgcca gcgaacttgg caaccaaaac agcacgacac
    16561 aggaaggcgg ttggtcgctc gcgtcattaa ctaacttgct tagcagcggg aaccaggcct
    16621 taagcgcaga taacatgaac aacgccgcag gcattctgca atactgcgcg aagcaaaagc
    16681 tggcgtcggt aaccgatgcc gaaaacatca agaaccaggt gctggaaaag ctgggcctga
    16741 acagtgaaga gcaaaaagaa gacaccaact atctggatgg cattcagggt ttgctgaaaa
    16801 ccaaagatgg tcagcaactc aatctggata acatcggaac gactccgctg gcagaaaagg
    16861 tgaaaaccaa agcctgcgat ctggtgttaa agcaggggct gaacttcatt tcctgaaccg
    16921 cgaaacgccg cgttttagct gttacccggc tgacgcggct ctctcctgcc cttttccctg
    16981 cttctggacg gttttccccc tgttacacgg gaagcgaccg cgatcaaaaa agtctgttta
    17041 taaactaatt cctaacaaca tcgcattttt ttgacactag aattgcagca atatggcgac
    17101 gccattacat tgttcttctc aaaaagctta aacgttggct aaacggcccg gtttagtgag
    17161 gatcgcccgt tgtcagaact actctctttc gccctttttc tcgcctctgt gctgatttac
    17221 gcatggaaag cgggacgtaa cacctggtgg tttgcagcca cattaacggt gctggggcta
    17281 tttgtcgttt taaatatcac cctgtttgcc agcgactatt ttactggcga tggtattaac
    17341 gacgcggttc tctatacctt aaccaacagc ctgaccggtg ctggcgtcag caaatacatc
    17401 ctgccgggta tcggcattgt gctggggctg acagcggtgt tcggtgcgct gggctggatc
    17461 ctgcgccatc gtcgccatca tccgcaccat tttggttaca gcctgctggc gctcttactg
    17521 gcgctgggtt cggtggacgc cagcccggca tttcgtcaga taacggaact ggtgaaatcc
    17581 cagtcacgcg atggcgaccc ggactttgcg gcttattata aagagccgtc gaaaactatc
    17641 cctgacccga aactcaacct ggtttatatc tacggcgaaa gtctcgagcg gacctatttt
    17701 gataacgaag ctttcccgga tctcacgcct gaactgggcg cgttgaaaaa tgaaggcctg
    17761 gatttcagcc acacgcagca gctgccagga acggattaca cgattgcggg catggtggct
    17821 tctcagtgcg gcataccgct gtttgccccc tttgaaggca acgcctccgc ctctgtctcc
    17881 agcttcttcc cgcagaacat ctgtctgggc gatatcctga aaaactcggg ttatcagaac
    17941 tatttcgtgc agggcgcgaa tctgcgtttt gccggtaaag atgtgttcct gaagtcgcat
    18001 ggcttcgacc acttatacgg ctcagaagag ctgaaaagcg tggtggccga cccgcactat
    18061 cgcaacgact ggggattcta cgacgatacc gttctcgatg aagcgtggaa aaagtttgaa
    18121 gagctttccc gctcaggtca gcgattctca ctgtttaccc tgacagtcga tacccatcac
    18181 ccggatggtt ttatctcgcg tacctgtaac cgcaaaaaat atgattttga cggtaagccg
    18241 aaccagtcat tcagcgcggt aagttgcagc caggagaata tcgcggcgtt tatcaacaaa
    18301 atcaaagcgt caccgtggtt taaagatacc gtcatcgtcg tctcttctga ccatttagcg
    18361 atgaacaaca cggcgtggaa atacctcaat aagcaggatc gcaataacct gttttttgtc
    18421 attcgtggcg acaagccgca gcaagagacg ctggcagtga agcgtaacac gatggataac
    18481 ggcgcgacgg tgctggacat tctcggtggc gataactatc tcggacttgg tcgtagcagt
    18541 ttatccgggc agtcgatgtc ggaaatcttc ctcaatatca aagagaaaac attggcgtgg
    18601 aagccggata tcatccgcct gtggaaattc cctaaagaga tgaaagagtt caccatcgac
    18661 cagcagaaaa acatgattgc cttctcgggt agccatttcc gtttgccgct gctgttgcgg
    18721 gtttcagaca aacgcgtgga accgctgccg gaaagcgaat actcagcacc gctgcgtttc
    18781 cagctggccg atttcgctcc acgcgacaat ttcgtctggg ttgaccgttg ctataagatg
    18841 gcacaactct gggctccgga actggcactc tccaccgact ggtgtgtctc gcaagggcag
    18901 cttggcggtc agcaaattgt tcaacatgtt gacaaaacaa catggcaggg caaaacggca
    18961 tttaaagata cggtcatcga catggcgcgt tataaaggca atgtcgatac gctgaaaatt
    19021 gttgataacg atattcgcta caaagccgac agtttcatct tcaacgtcgc cggtgcgcca
    19081 gaagaggtga aacagtttag tgggatttcc cgtccagagt cgtggggccg ctggtccaac
    19141 gcgcagctgg gcgatgaagt aaaaatcgag tacaagcatc cgctgccaaa gaaatttgac
    19201 ctggtgatta ccgccaaagc atacggcaat aacgccagcc gtcctattcc ggtacgcgta
    19261 ggcaatgaag aacaaaccct tgtgctgggc aatgaagtga ccaccaccac actgcatttc
    19321 gataacccaa ccgatgccga cacactggta attgtgccgc cggaacctgt ctcaaccaac
    19381 gaagggaata tcctcggaca ctcgccacgt aagctcggga tcggcatggt ggaaattaaa
    19441 gtggtggaac gtgaaggata attcctgact gatgtgtagg tcgcataagg tgcgacctac
    19501 acaccttctg cggcctacaa aacatcattg agaaacagaa aaaaacagcg cccaacactt
    19561 ccccggacgc tgcatggcaa ctttaataat ctcgccttat cagaaagtaa ttacaccctt
    19621 aatcaactca cgattgttaa tcacatcgcg ctcgtaggtt tctgccagcg tggcgaacgg
    19681 atagcgatgg gttaacatca tgtcagcagt gatttttcct tccgccatca gtcgaccgac
    19741 tttagcaaaa tcttccggcg tggcgttgcg gctacccatc atcgtcgttt cttttttatg
    19801 gaattccgga tcggagaact gcaactcacc tttaaacagg ccgacaaata ccaccgtgcc
    19861 gccgtgacga atcaaattca cggtgttatt catcgcatgt tgattacctg tcgcgtcgat
    19921 cactttctgc gccagcgaac caccaaactg cgcccgtagc tgcgcgtcaa aatcttcggc
    19981 tgacgggtcc agtaaaggta attccagacg cgttgccaca tgttcacggc gcgccggact
    20041 ggtatccgcc accaccacct gtgcgccatc ggctttagcg attgccgccg cgcccagacc
    20101 gattggcccc gccccgacca ccagcacctg ctcgccggga gcaatggctg cgcgacgcac
    20161 cgcatgagcg ctaatagcga aaggttcaat caatgctgcc gcctgcgggt caataccgtc
    20221 tgcgggcaaa atgttcgcca ccggcacgct caaatactca ctaaaaccgc catcctgatg
    20281 cacgccaatg actgaaattt tttcacagca attggtacgc ccgcttttac acgccgggca
    20341 ttgctgacag gcaacataag ggatcacagc aacttgcaga ccatttttaa gatcagcaat
    20401 atttttaccc agcctaacaa tctccccaca tatttcatgg cctaaaacac gtggataact
    20461 aaaaaatggt tgatttccac cccaggcatg aatatcggta ccgcaaatcc cgacagactt
    20521 aatttttatt aatgcttcat tgtcacccgg aataggtatc tcgcgttgtt tccagactaa
    20581 ttctttcggc tgctggcaaa ttaaaacatt catcgtagac atgattcggc tctccatttt
    20641 ttgttgcctt agatatcaat gaaaatgaaa aatggcagcg gaacggaaaa tctgttttgt
    20701 gaaaacacac gcataaaaat gttttaaatc gggttttaat ggactcacaa ggaggaaaat
    20761 atgagtcgtt cacaaaattt acgccacaat gtgattaacc aggtcattga tgatatggcg
    20821 cggggtcata tcccttcccc acttccatcg caaagtgcgc tggcggaaat gtacaacatc
    20881 agccgcacca cagtgcgtca tattctcagc catttacgcg aatgcggcgt cttgacgcag
    20941 gttggaaacg actatgttat tgcgcgtaaa cctgaccacg atgacggttt cgcctgtact
    21001 accgcttcaa tgagtgaaca aaacaaagtg tttgagcagg cttttttcac gctgataaac
    21061 cagcgccagt tacgcccagg ggaaactttt tctgaactgc aactggctcg ggccgcgggt
    21121 gtcagcccgg tggttgtaag agaatatctt ttaaaattcg gccgttataa tcttattcaa
    21181 agcgaaaagc gcggccagtg gagcatgaag caattcgatc aatcctacgc cgagcagctg
    21241 tttgagctac gcgaaatgct cgaaacacat tcacttcagc actttctcaa cctgcctgat
    21301 catgatcccc gctggctgca ggcgaaaacc atgctcgaac gtcatcggct actgcgtgac
    21361 aacatcggca atagctttcg catgttctcg caacttgaca gagattttca ctccttactg
    21421 ctttcagctg ccgacaatat cttttttgat caatcgcttg agatcatctc agtcatcttt
    21481 cattttcact atcaatggga cgaaagcgat cttaaacagc gcaatatcat tgccgtcgat
    21541 gagcatatga ccattctcag cgctctgatt tgccgcagtg acctcgatgc cacccttgcg
    21601 ctccgtaacc atttgaattc agctaaacaa tcaatgattc gatccattaa tgagaacacg
    21661 cgttatgctc attaagtacg gattaaaaac agcaaactat tagcgggtgc acggttttat
    21721 caaaagataa ataccgggaa attcccgctt acctatgctc acaatcagtc gactaaaaat
    21781 gtttttgtga gcggtagtaa agtcctaaaa ctttaacctg ccgttaatgt caaccttgcg
    21841 taatgcacaa ttacgtaaac aattaaaatt ataaatacag gagtccggcg tggaaaaaga
    21901 aaatatcacc atcgatccgc gttcttcatt tactccatct tcgtcggcag atattcccgt
    21961 gccaccagat ggattagttc aacgcagtac ccgaattaaa cgcattcaaa ccaccgccat
    22021 gttgttatta ttttttgcgg cggtaatcaa ttatctcgac cgcagttcgc tgtcggtagc
    22081 aaatttaacg attcgtgaag aattgggatt aagtgccact gaaatcggcg ctttgctctc
    22141 cgtgttttca ctcgcttacg ggattgcgca acttccttgc ggcccactat tggatcgtaa
    22201 aggcccacgc ctgatgctgg gactggggat gttcttctgg tcactgttcc aggcaatgtc
    22261 tggcatggtg cacaacttta cgcagttcgt gttggtgcgt atcggtatgg ggattggtga
    22321 agcgccgatg aacccatgcg gtgtaaaagt cattaacgac
//
LOCUS       DAJTMC010000059        22184 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.059, whole genome shotgun sequence.
ACCESSION   DAJTMC010000059 DAJTMC010000000
VERSION     DAJTMC010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22184
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.059"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            100..682
                     /locus_tag="OO130_001966"
                     /pseudo
     CDS             100..682
                     /locus_tag="OO130_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417001.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
     gene            complement(777..2354)
                     /gene="guaA"
                     /locus_tag="OO130_001967"
     CDS             complement(777..2354)
                     /gene="guaA"
                     /locus_tag="OO130_001967"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HCR5365394.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMHMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYNKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(2423..3889)
                     /gene="guaB"
                     /locus_tag="OO130_001968"
     CDS             complement(2423..3889)
                     /gene="guaB"
                     /locus_tag="OO130_001968"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HCR5365395.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            4051..5421
                     /gene="xseA"
                     /locus_tag="OO130_001969"
     CDS             4051..5421
                     /gene="xseA"
                     /locus_tag="OO130_001969"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HCR5365396.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     TRQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRTGQQQQRLTQRLNQQNPQPKIHRAQTRIQQLEYRLAETLRVQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            complement(5418..5633)
                     /locus_tag="OO130_001970"
     CDS             complement(5418..5633)
                     /locus_tag="OO130_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HCR5365397.1"
                     /translation="MELHCPQCQHVLDQDNGHARCPSCGEIIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(5702..7174)
                     /gene="der"
                     /locus_tag="OO130_001971"
     CDS             complement(5702..7174)
                     /gene="der"
                     /locus_tag="OO130_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HCR5365398.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEVEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            complement(7292..8470)
                     /gene="bamB"
                     /locus_tag="OO130_001972"
     CDS             complement(7292..8470)
                     /gene="bamB"
                     /locus_tag="OO130_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HCR5365399.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYD
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(8481..9101)
                     /gene="yfgM"
                     /locus_tag="OO130_001973"
     CDS             complement(8481..9101)
                     /gene="yfgM"
                     /locus_tag="OO130_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HCR5365400.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(9119..10393)
                     /gene="hisS"
                     /locus_tag="OO130_001974"
     CDS             complement(9119..10393)
                     /gene="hisS"
                     /locus_tag="OO130_001974"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HCR5365401.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDLNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGISEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPIVDIYLV
                     ASGADTQSAAMALAERLRDELQGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     TNGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(10504..11622)
                     /gene="ispG"
                     /locus_tag="OO130_001975"
                     /gene_synonym="gcpE"
     CDS             complement(10504..11622)
                     /gene="ispG"
                     /locus_tag="OO130_001975"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HCR5365402.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(11649..12662)
                     /gene="rodZ"
                     /locus_tag="OO130_001976"
     CDS             complement(11649..12662)
                     /gene="rodZ"
                     /locus_tag="OO130_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HCR5365403.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLLGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPAPTAATTPDGAAPLPTDQAGVTTPVADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            complement(12947..14101)
                     /gene="trmG/rlmN"
                     /locus_tag="OO130_001977"
     CDS             complement(12947..14101)
                     /gene="trmG/rlmN"
                     /locus_tag="OO130_001977"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HCR5365404.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(14251..14682)
                     /gene="ndk"
                     /locus_tag="OO130_001978"
     CDS             complement(14251..14682)
                     /gene="ndk"
                     /locus_tag="OO130_001978"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HCR5365405.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            complement(14831..17143)
                     /gene="pbpC"
                     /locus_tag="OO130_001979"
     CDS             complement(14831..17143)
                     /gene="pbpC"
                     /locus_tag="OO130_001979"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HCR5365406.1"
                     /translation="MLRLLTKRGCWITLAAVPFLLFLAAWGADKLWPLPLHEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKILQLWRALQLEWHLSKREILILY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAA
                     RNKVLERMAVQGVWSREQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLEWPLMSSGAAWIIRRIMADEAQPLPDSALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSANLPEDPRPNSVTRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLPEQEG
                     INGIRFPIWLDENGKRVAADCPQARQEMINVWPLPLEPWLPASERRAVRLPPASTSCP
                     PYGHYAQLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIATVKFVMQ"
     gene            complement(17144..22105)
                     /gene="yfhM"
                     /locus_tag="OO130_001980"
     CDS             complement(17144..22105)
                     /gene="yfhM"
                     /locus_tag="OO130_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HCR5365407.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     ASSAKLSVPERQKLAQQSAGKVLTLLDLSEVQLDGAATLVLTFSIPLDPDQDFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDYEKTIT
                     TRDIQPSVGFASRGSLLPGKVVEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAAQQGIEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPDQPIKLDVIKPDGQVLRSVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPNDEVKFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPANALPGLRPQFALK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     DEGWQSQFDQKDLIENEQTLDLKADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGSGAVRPDRVTLKLDKASYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVPAQGLDLTIPVDKTWNRHDLYLSTLVVRPSDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPAKMRPNQPLTVKIKASTKNGEKPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPLVNHVNIVAQQA
                     LPVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADDFGSNESKVIVAAPVIAELNMPRF
                     MASGDTSRLTLDITNLTDKPQKLNVALTASGLLELVSDSPAAVELAPGVRTTLFIPVR
                     ALPGYGDGEIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAI
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMMSIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGIALKTMGDATRGEEAIVLALKTPRNSDERIW
                     LGDYGSPLRDNALMLSLLEENKLLPDEQYTLLNTLSQQAFGERWLSTQESNALFLAAR
                     TIQDLPGKWQAQTSFSAEPLTGEKTLNSNLNSDQLATLQVRNSGDQPLWLRMDASGYP
                     QSAPLPANNVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     AGLELENQNLANGSASLEQSGDEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAAEDLLIVRP"
BASE COUNT         5281 a         6221 c         5498 g         5184 t
ORIGIN      
        1 acgacaggtt tagccgttgt tattaccaca acgtataacg aaacgcgtat tcagccaaat
       61 actaaatgtc ctgaaaagag ctaataatca ggaggagtca tgaagaaagt ttttctttgc
      121 gccatcttgg cctccttaag ctatccggct atcgcctcat cattgcagga tcaactctct
      181 gctgtagcag aagcggaaca gcaaggtaaa aatgaagagc aaaggcagca tgacgaatgg
      241 gtcgcggagc gcaacaggga aatccagcaa gagaagcaac gtcgcgcaaa tgcccaggtc
      301 gccgctaaca aaagagcggc aacgtcgcgc aaatgcccag gtcgccgcta acaaaagagc
      361 ggcaacggca gcagcaaata agaaagctcg tcaggataaa ctggacgccg aagcctctgc
      421 ggacaaaaaa cgcgatcaaa gttatgaaga tgagctacgc agcttagaga ttcagaaaca
      481 aaaactggcg ctggcaaaag aagaagcccg cgttaagcga gaaaacgaat ttatcgatca
      541 ggaactgaag cacaaagctg cgcaaaccga tgtggtgcaa tctgaagctg acgccaacag
      601 aaatatgact gaaggcggtc gcgatctgat gaaaagcgtg ggcaaagcag aagagaacaa
      661 atcggacagc tggtttaatt aatcgatgtt agtaacttca agcctatgat tcttgaagat
      721 aaaaaaccct ctgtagtaac agagggtttt gttcattcat agtgcaggga ttaaaatcat
      781 tcccactcaa tggtagctgg cggcttgccg ctgatgtcat acaccacgcg ggaaataccg
      841 ttcacttcat tgataatgcg gttggaaacg cgaccgagga aatcgtacgg cagatgcgcc
      901 cagtgtgcgg tcataaagtc gatggtttcg acagcacgca gagagacaac ccagtcatac
      961 ttacgaccat cgcccattac gccaacggaa cgtaccggca ggaacacagt gaacgcctgg
     1021 ctgactttgt tgtacaggtc cgctttacgc agttcttcaa tgaagatggc gtcagcacgg
     1081 cgcagcaggt cacagtactc tttcttcact tcacccagca cacgaacgcc aaggcctggt
     1141 cccgggaacg ggtgacggta cagcatgtcg tacggcaggc ccagctccag accaatctta
     1201 cgcacttcgt ctttgaacag ctctttcagc ggttcaacca ggcccatctt catctctttc
     1261 ggcaggccgc ccacgttgtg gtgagatttg atgacgtgtg ctttaccggt tgcagaagcc
     1321 gcagattcga taacgtcagg gtagatggtg ccctgcgcca gccacttcac gtcttccagt
     1381 ttcagcgctt cttcatcgaa cacttcaacg aaaacgcgcc cgatgatttt acgttttgct
     1441 tccggatcgt tttcgccagc cagcgctgac aggaagcgat cttctgccgg tacgtgaaca
     1501 atgttcagac caaagtgatc gccaaacata tccagaacct gctctgcttc gttgagacgc
     1561 agcaggccgt tgtcgacgaa tacgcaagtc agatttttac cgatagcgcg gtgcagcagc
     1621 attgcggtta cggaggaatc cacaccacca gagaggccga ggatgacttt atcgtcgcct
     1681 acctgctcgc ggatgcgagc tacagcatcg tcgataattt tcgctggcgt ccacagggct
     1741 tcacactggc agatatcacg cacaaaacgc tccagcatgt gcataccctg gcgggtgtga
     1801 gtcacttccg ggtggaactg tacgccatag aagcgttttt cttcgttagc cataatggca
     1861 aacgggcagc tttcggtgct ggctacggtg acgaagtcgg acgggatagc ggtaacttta
     1921 tcgccgtggc tcatccagac atcgagcagc ggtttaccgt ctgcggtcag cgcatcttcg
     1981 ataccgcgaa ccagtgcgct gtcgtttacg acttcaacct gcgcgtagcc aaattcacgt
     2041 tcgttagagg cttcaacgtg accgcccaac tgcattgcca tggtctgcat gccatagcaa
     2101 acgccgaata ccggtacgcc tgcttcaaag acatactgcg gcgcacgcgg actgttttct
     2161 tcagtagtac tttccgggcc gccggaaaga ataatgccgc ttggattgaa gtcacgaatt
     2221 tgtgcttctg tcacatccca cgcccacagt tcgcagtaaa cacccagctc acgcacgcgg
     2281 cgcgcaacca gttgagtgta ctgagaaccg aagtccagaa tgaggatgcg atgcttatga
     2341 atgttttccg tcattgacgc ttattccgag gcaagtgaaa cagataatat aaatcgcccg
     2401 acatgaagtc gggcgaagag aatcaggagc ccagacggta gttcggggac tctttagtaa
     2461 tggtcacgtc gtgaacgtgg ctttcctgaa tgcccgcacc gctgatacgt acaaactccg
     2521 ctttagtacg cagttcgtcg atagtaccac agccggtcag gcccatacag gagcgcaggc
     2581 cgcccatctg ctggtgaatg atctctttca ggcgaccttt ataggctacg cgaccttcga
     2641 taccttccgg caccagtttg tcggcagcgt tgtcactctg gaagtaacgg tcagaagaac
     2701 ctttggacat cgcacccaga gagcccatac cacggtaaga tttgtaagaa cggccctggt
     2761 agagttcaat ttcacccgga gattcttcag tacccgccag catggaaccc accattaccg
     2821 cgcttgcgcc agcggcgata gctttggcga tgtcgccgga gaagcgaata ccaccatctg
     2881 cgataaccgg aatacctgtg ccttccagcg cttcaactgc gtcagcaacg gcagtgatct
     2941 gcggaacacc tacgccagta acgatacgag tcgtacagat agaaccaggg ccgataccga
     3001 ctttaaccgc gctgcaacca gcttctgcca aagcgcgtgc acctgcagct gttgccacgt
     3061 tgccgccgat gatttgcaga tccggatatt tagcacgggt ttcacggata cgctgcaaaa
     3121 cgccttctga gtggccgtgg gaggagtcga tcagcagaac gtcaacgcca gcggcaacca
     3181 gcgcgtcaac acgctcttcg ttacccgcac ctgcgccaac tgcagcacca acacgcagac
     3241 ggccttgctc gtctttacag gcgttcggtt tacgttccgc tttctggaag tctttcacgg
     3301 tgatcatgcc gatcaggtgg aattcgtcat caaccaccag cgctttttca acgcgttttt
     3361 cgtgcatttt tgccagcacc acttcacggg cttcaccttc acgcacggtg accagacgct
     3421 ctttcggcgt catgtaaacg ctaaccggct ggttcaggtc ggtaacaaaa cgcacgtcac
     3481 gaccggtgat aatacccacc agttcgtttt cttcggtaac aaccggatag cccgcaaaac
     3541 cgttacgctc ggtcagttct ttcacttcgc gcagcgtcgt ggttggcaga acagtctgcg
     3601 gatcagtcac cacaccagat tcgtgttttt tcacacggcg aacttcttct gcctggcgtt
     3661 caatggacat gtttttgtgg ataaagccga taccgccttc ctgagccaga gcaatagcca
     3721 ggcgcgcttc cgttacggta tccattgctg cggaaagcat agggatattc agacgaatag
     3781 ttttcgtcag ctgggtgctg aggtcagcag tattcggcag aacggtagaa tgagcaggaa
     3841 cgaggagaac gtcgtcaaac gtcagagctt ctttagcgat acgtagcatg ggcaatatct
     3901 cgaccagagt ggttaataaa tattgccgcg gcattataca gagcgtaacc gattgcatct
     3961 accccttttt gcaaaaaatg cttgctatcc ccgaagggcg ggttactatc gactgaataa
     4021 cctgctgatt tagaatttga tctcgctcac atgttacctt ctcaatcccc tgcaattttt
     4081 accgttagtc gcctgaatca aacggttcgt ctgctgcttg agcatgagat gggacaggtt
     4141 tggatcagcg gcgaaatctc taatttcaca caaccggctt ccggtcactg gtactttaca
     4201 ctcaaagacg acaccgccca ggtacgctgc gcgatgttcc gcaacagcaa ccgccgggtg
     4261 accttccgtc cacagcatgg acaacaagtt ttagttcgcg ccaatattac gctctacgag
     4321 ccgcgcggtg actaccagat aatcgttgag agtatgcagc cggccggtga agggctgctg
     4381 caacagaagt acgaacagct caaagcgaag ttgcaggctg aaggtttgtt cgatcagcaa
     4441 tacaaaaaac cacttccctc ccctgcgcat tgcgttggtg tgatcacctc aaaaaccggt
     4501 gctgcgctac atgatatttt gcatgtgtta aaacgtcgcg atccttctct accggtgatc
     4561 atctacccca ccgccgttca gggcgatgac gcaccggggc aaattgttcg cgccattgaa
     4621 ctggcgaatc agcgcaatga gtgcgacgtg ttgatcgttg ggcgcggcgg cggttcgctg
     4681 gaagatttat ggagttttaa cgacgaacgc gtagcgcggg cgatttttgc cagccgcatt
     4741 ccggtcgtca gcgccgtcgg gcatgagacg gatgtgacca ttgccgattt tgttgccgat
     4801 ctgcgtgcgc caacgccgtc tgccgccgct gaagtagtga gccgtaatca gcaagagtta
     4861 ctgcgccagg tgcaatcgac ccgtcaacgg ctggagatgg cgatggatta ttatctcgcc
     4921 aaccgcacgc gtcgctttac gcagatccat caccgattac agcaacagca tccgcagctc
     4981 cggctggcac gccagcaaac catgcttgag cgcctgcaaa agcggatgag ctttgcgctg
     5041 gaaaatcaac ttaagcgtac cgggcaacag cagcagcggt taacacagcg gctgaatcag
     5101 caaaatccac agccgaagat tcatcgcgcg caaacgcgca ttcagcaact ggaatatcgt
     5161 ttagcagaaa ccctgcgcgt acagcttagc gccacgcgtg aacgtttcgg taatgcagta
     5221 acgcacctcg aagccgtaag cccactgtca acgctggcgc gtggatacag cgttactact
     5281 gctactgacg gcaatgtact gaaaaaagtg aagcaagtta aagcgggtga aatgctaacc
     5341 acacgtctgg aagacggctg gatagaaagt gaagttaaaa acatccagcc agtaaaaaaa
     5401 tcgcgtaaaa aggtgcatta agccagcacg aattcaacgc gttttttcga tatcaagcca
     5461 tgcccgtgct gacagaagta atctactgca ccgcaggctt ttaacacctg caacggttga
     5521 tggcagtctg gacaaagagc tttcatttca ataatttccc cgcaggatgg gcaacgcgca
     5581 tggccgttat cctgatcaag cacatgctga cattgtggac aatgcagttc catatgaatt
     5641 cctccggtag ccatccctct acattcatag agggaatggc agataaaata cttacggata
     5701 attatttatt tttcttgatg tgcttcatca gacgcttacg tttacgcatc tgggttggcg
     5761 tcagggtgtt acgcttgttc gcatacgggt tttccccttc tttgaactga atacgaatcg
     5821 gcgatcccat tacgtccagc gatttgcgga agtagttcat caagtagcgc ttgtaggaat
     5881 caggcaggtc tttcacctga ttaccgtgaa tcaccacaat cggcgggtta taaccaccag
     5941 cgtgggcata tttcagcttc acacgacgac cgcgtaccag cggcggttgg tgatcttcaa
     6001 cagccatcgt catgatgcgc gtcagcatag aggtccccac acgacgggtg gagctgtcat
     6061 acgcttcacg tactgattca aacaagttac caacaccact gccgtgcaag gcagagataa
     6121 agtgcacacg agcaaaatcg ataaagccca gacggaagtc cagcgtttct ttcacctgct
     6181 ctttcacttc ctggctcagg ccatcccact tattcaccac aatgacaagt gagcgcccac
     6241 tattgagaat aaagcccagc agcgagagat cctgatcgga aataccttcg cgcgcatcaa
     6301 tcactaacat caccacgttg gcatcttcaa tggcctgcaa cgttttgatt acggagaatt
     6361 tctctacagc atcggtgatt ttgccgcgtt tacgtacgcc agcggtgtca atgagcacat
     6421 actcacgtcc atcgcgttcc attggaatgt agatactgtc acgcgtcgtg ccaggcatgt
     6481 cataaacaac aacgcgctct tcaccaagaa tacggttagt gagtgtagac ttacctacgt
     6541 tcggacgacc cacaatcgcc agtttgatcg gcagactttg cgggtcgaag tcgtcttcct
     6601 cttcttcttc gccgttctct tccacttcaa attgcgccca gtattcagcg tcttcgtcga
     6661 cttcctcttg cggtgcgaga tcttccatcc acggcagcag cacatgctcc agcagactta
     6721 atacgccacg accgtgagac gcggcgatcg ggtagatttc acctaagcca agcgagtaga
     6781 aatcaaccac tgcctgatcg ggatccagac cgtcagtttt gtttgccacc aggaaggtcg
     6841 gtttttcacg ggagcgcaga tgtttggcaa tcgcttcatc tgccggcatc aggcccgcgc
     6901 gcgcatccac cataaacagt acgacgtccg cttcttcaat cgccagcagc gactgttccg
     6961 ccatgcgggt ttctacaccg tcttctgtgc catcaatccc gccggtatca atacagataa
     7021 actcacggcc ttcaatttcc gcacgaccgt acttacggtc acgagtcaga cccgggaaat
     7081 ccgcaaccag cgcatctcgg gtgcgagtta gacggttaaa taacgtggat tttcctacgt
     7141 tagggcgccc gacaagcgcg accacaggta ccatgtttaa agcctcattt ttataaatca
     7201 tcagacaacg cacgctatat tcgcgtcgtt gttaaaaaca ggaaaacggc ccctgtccag
     7261 gagccgtttt caaagtgaac gacagagacg attaacgtgt aatagagtac acggttccgt
     7321 cttttgcctg gatcagcagt ttgccgtcag cggcaaccgg ttcagtctgg aaaccggaac
     7381 tatcaacttt ttgctgggca acgaaacgac catcttcgac attaatccag tgcagataac
     7441 cttcactgtc accgaccacc aggttgccat catacagcac cggagaagtc agcaggcgat
     7501 gcaacagatc gctttgtgtc cacagcgtaa cgccgccatc aatggtcaac gccatcaccc
     7561 ggtcattttg atcgaccaga tagatgcgat tgccgtcgac gatgaaatca ttcaccgaac
     7621 ccagttcgcg tttccacata atctgaccac tgcgcagatc aagcgccgtc aggttaccat
     7681 tataggccag cgcgaaaaca acgccgttaa caacgacggg agtcgtgtca acatcgctca
     7741 gacggtcaat ttcggtagaa ccggtcgcct gggaaatacg ctgctgccaa atcatctggc
     7801 cctgttccat cagcactgcg ctgacgcgac cattatcgcc ccccacgacg gccgcaccaa
     7861 aagccgttgc cggcgcagac tcgccacgca aagagagcga aggcatatcg aggttaactg
     7921 tccatttgac agcgccgtca gcttcgttca gcgcttgtaa ctgaccgtta ctggtgtgga
     7981 ttaacaccag accgtcgctg accaccgggc gcgaaagtgc ttcacccgcg actttagttt
     8041 gccatgccac agtaccatcg ctggtattca gcgcgtaaac ctgcgccttt tcgctgccaa
     8101 tgtagacatg cccaccagac acggtcacac cgccagaaag taatgcaggc tctttagaga
     8161 accagccatc tttctcggcc aggctgacag accagatttc tttgccatca tccgcattca
     8221 gcgcttttac caaaccagcg cggtccgctg catagacaac gttgtccgcc agtgccggat
     8281 gaagattgga atagaagttg ccaatgccgc taccaacgga agtactccac gccgtggtcg
     8341 gcgtaaactg gttttcaacg gttggcaatg gggacatctt taccacatct tcttcgctgt
     8401 taaacagcga acagccgctt aaaagggtaa cggaaagcag tcctggcagc agtaatttac
     8461 gcaattgcat cgggtccctc tcagatggac aaattattaa ttttcatctg catcatttcg
     8521 ctcagtgccg gagtaacatc gcttttcacg cctgcttccc atgcactacg cgcaccttgc
     8581 ttatcacctt tgctcagcaa tgcttcacca cgcaggtcgg caacaatggc agcccaccct
     8641 tcacctttga tggtatcaag ggttttcagc gcggcatcag cctgcttgag ctgtacctga
     8701 acgcgagcaa gacgcagatt tatcacggct ttgagatttt catcgctcgt gtctgccagc
     8761 ccctgttgta actgggcggc agctttctct agttcatttt tgtcaacaaa ttgctgcgca
     8821 agttccaaag aagccagcgc accataagta tttttatttt cagcagcaaa tttttccgcc
     8881 gccgggatgc tatccggttt gccttcgctc actgcggtaa ccgcattttg ataggcaaga
     8941 gaagcggagc gtgcagaatc aacctgatgg ctgttccagt agcgccagcc aatcagtgcg
     9001 ccaacgccca aaatcacccc aacagccagt gctttgccat tttcagcaaa aaagcgttta
     9061 accgcttcta cctggtcgtt ttcgttctcg taaatttcca cgctgtcctt ctccttcctt
     9121 aacccagtaa cgtgcgcaaa tgcgcggcta cgctatcctg cgcaactgcc gtttgctcac
     9181 cagagcgcaa atccttcact actgctgtgc cgttagtcac ttcagactca cccagcacca
     9241 cagcaacgcg ggcaccccat ttatcagcac gggcaaactg tttcttaaag ttgccgccgc
     9301 cgtggttggt catcaatttc acgccctgta attcatcacg cagacgctca gctaatgcca
     9361 tagccgcaga ttgtgtatca gcacctgaag ccaccaggta tatatcgaca ataggatcgg
     9421 ctttaaattc cggattaacg gcctgtacta acaatacaag acgttcgagg cccatcgcaa
     9481 aaccgacagc cggtgttgca cgaccgccca gttgttccac aagaccgtca taacgaccgc
     9541 ctgcacacac ggtgccctgg gagccgagac tgttagtcac ccactcgaaa acggtacggt
     9601 tatagtaatc cagaccacgc accagacgct ggtttacggt gtaagcgatc cccgcgctct
     9661 caagcagttt gcacagaccg gcaaaatgct cacgagattc ctcgtccaga taatcaccta
     9721 atgccggagc gtcgttgaga agcgcctgca cttccggatt ttttgaatcc agcacgcgca
     9781 gcgggttagt gtacatgcgg cgtttgcagt cttcgtccag cttttcttta tgctgctcaa
     9841 ggaatgccac cagcgcatcg cggtaattgg cgcgtgcttc cagcgaaccg atagaattca
     9901 gctcaagagt tacgtgttcg gaaataccca gcgcgcgcca ccagcgggcg gtgagcataa
     9961 tcagttcagc gtcgatatcc ggaccttgca gaccgaaaac ttcgcagccc aactgatgga
    10021 actgacgata acgccctttc tgcggacgct cgtgacggaa catcggcccg atataccaca
    10081 gacgctgttc ctgattgtac agaagaccat gctcgatgcc ggcgcgtaca cagcccgccg
    10141 tcccttcagg gcgcagagtc aggctgtcgc cattgagatc ctcaaaggtg tacatctctt
    10201 tttcaaccac gtcggtgact tcaccaatcg cacgtttgaa tagcggggtc tgctctacaa
    10261 tcggcaagcg gatttcactg taaccgtagc tgccgagcac gtttttcagt gtgccttcaa
    10321 tgcgctgcca gatggccgtt tcgccaggca ggtaatcgtt catgccgcga atggcttgaa
    10381 tgttttttgc cacgtttatt ctctttctga atataaaaat gaaccctcaa cgcttccctc
    10441 aatgtttggg gagccatgcg ggttcaatca tacacgggaa gcgaggcgct tcccatcacg
    10501 ttattatttt tcaacctgct gaacgtcaat tcgacgcgct tcatccagct gactggcttt
    10561 cgcacgaatg cgtgcttcca gctggtcgat catatcgttg ttgtccagac ggtctttgcg
    10621 cacgccatct tcatagaggc cgcttttctt gttgccgccg gtgacgccga gtgtagaaac
    10681 cagcgcctca cctgggccat tcaccacgca gccgataata gaaacgtcca tcggagtgat
    10741 gatatcttcc aggcgttgct ccagcgcgtt aaccgtaccg ataacatcaa attcctgacg
    10801 cgaacaggtc gggcaggcga tgaagttgat ccctcgcgaa cggatacgca gcgatttcaa
    10861 aatatcgaaa ccgactttga tctcttcgac cggatcggcc gccagcgata cgcgcagcgt
    10921 gtcgccgatg ccttcagaca gcagcagacc taaaccaatg gcggatttta ctgccccgct
    10981 gcgtgcacca ccggcttcgg tgatccccag atgcagcggc tgatcgatct gttttgccag
    11041 caaacgataa gactcaacag cgaggaagac gtcagacgct ttcacgctga ctttgaactg
    11101 atcgaagttc aggcgatcga gatgatcaac atgacgcatg gcggattcca gcaacgcctg
    11161 cggcgtcggt tcgccatact tttcttgcag atctttttcc agcgatccgg cgttaacgcc
    11221 aatacggatc ggaatgtttt tatcgcgcgc acagtcaacc accatgcgaa tacgctcttc
    11281 attaccgata ttgccagggt taatacgcag acaatcgacg ccgtattccg ctactttcag
    11341 cgcaatgcga tagtcgaagt ggatgtcagc caccagcggc acgttaaccc gctgtttgat
    11401 gagtttgaac gcttctgccg cgtccatcgt cggtacggag acacggacga tatcagcgcc
    11461 aacgcgttcc agcgccttga tttgattgac cgttgcttcg acatctgtcg tacgcgtatt
    11521 ggtcatggac tgtacggcga tgggagcacc atcgccaatc ggcacattcc caacgtaaat
    11581 acgtgttgat tttctacgtt gaattggagc ctggttatgc atgaaaaatc tcccgcgtta
    11641 cccgtctgtt actgcgccgg tgattgttcg gcattgaggg tcagacgcgc aacctggtta
    11701 gttctgataa aacgactcag atcgacaggt ttcccttgat actggatctg tactgcggct
    11761 ggcgcaccaa ttttcagttt gtacggtgcc tggccggtta agttcaaatt accgtcttta
    11821 cgctgcatac cgctaaacaa ttttttaccg gtagcatcag tgacctccag ccagcaatcg
    11881 gcagtaaagt tcatcaccag cgcattcgga tcagccaccg gcgtggtcac gccagcctga
    11941 tcggttggca acggcgcagc accatctggc gttgttgctg ccgttggtgc cggggtcgcg
    12001 gcggtatcaa catttgcctg cgaaggcgaa acaaccgcat tctgttgcgg atcaacagct
    12061 ggtgctggcg cagttacggc aggtgtttgc gtgttggttg cggtagtatc cacagacgct
    12121 ggcggcgtgg acgttgtagc cgggtctgta gttgtcgacg tatttaacgg aacactctgc
    12181 ccctgctcgc tattactgct cagttccgcc gaagattgat cggccatagt ggtgatctct
    12241 tcctgctgag ctttgtggtc ttgccaccac caggcaccgc tcaggccgat aaccacaaac
    12301 aacaccagcc aggtgaaggt catcagccag ccgtcacgtt ttttgcggcg tttaccgagg
    12361 gaaaaactct gcatcggcgc aactttcgca gcccgtagtg gagcctgctt ttccagccct
    12421 agcagcagtt cttcttctgg aatatgtacc agacgcgcat aagagcggat atatccgcgc
    12481 aggaatgttg aagcaagatc ggcgggtgcc ttatcttctt caatgtcgcg taccgtggaa
    12541 accttcaggc aaagtcgctc ggcaacggcc tgctgactaa gtcctagttg ttcgcgagca
    12601 ttacgcaggc gagcgccggt agtaagtgct tcattttggt cgtgcgtggc ttcagtattc
    12661 attcgctgca ggtacgttta aatgagaatt gagatgccgg tgaaccatca tgctcaccca
    12721 caccgcgaaa catccgttaa gttaaccgtt atcatacagt ataagaccgt ctgtgtgctc
    12781 gtgacaaagc ccacattcac taaggctaac ttactgttgc atcgttacat actgccttaa
    12841 agtcagcaaa aacgcaccgt taatattgac cagacaattg caacttaata cttcattcat
    12901 aaaagattac gccacggtac ataaagtaac cgtggcgtaa tggctatcag accgctttaa
    12961 tgtcgatggc ttcaccctgc atccgtttac gcagggtacg tttcgtacgg tcgataacat
    13021 cgcccgccaa ctgaccacag gcagcatcga tatcatcacc acgagtttta cgcacaatag
    13081 tggtgaaacc gtagctcatc agcacttttg agaaacggtc gatacggctg ttcgaactgc
    13141 gtccatacgg cgcgcccggg aacgggttcc acgggatcag gttgatctta cacggcgtat
    13201 ctttcagcag ctccgccagt tggtgcgcgt gttcagtgcc gtcgttaacg tggtcaagca
    13261 tcacgtattc aatagtgact cggccctgat tggcgttgga tttctccaga taacggcgca
    13321 ccgcagcaag gaacgtttcg atattgtact ttttgttgat cggcacaatt tcgtcacgaa
    13381 tttcgtcgtt cggcgcgtgc agggaaattg ccagtgcaac gtcgatcata tcgcccagtt
    13441 tatccagcgc cggaactaca ccggaagtag aaagcgtgac gcgacgttta gacaggccaa
    13501 aaccgaaatc atccagcatg atttccatcg ccggaacgac gttgttcagg ttgagcagcg
    13561 gctcgcccat gcccatcatc actacgttag tgattgggcg ctgaccggtg acttttgctg
    13621 cgccgacgat tttcgccgca cgccacacct ggccgataat ttccgacacc cgcaggttgc
    13681 gattaaagcc ctgctgggcg gtggaacaga atttacactc cagcgcacac cccacctgcg
    13741 aagagacgca gagcgtggca cggtcgtctt ccgggatata caccgtttcg acgcgctgat
    13801 cgccaacggc gatcgcccat ttaatggtgc cgtcggatga acgctgttct tcaaccactt
    13861 ccggtgcgcg gatttccgcc acctctttca gtttgccgcg caacactttg ttgatgtcgg
    13921 tcatctcatc aaagttgtcg cagcaatagt gatacatcca cttcatcacc tgatcggcgc
    13981 ggaagggttt ttcacctaaa tctttaaaaa actcccgcat ctgctgacgg ttgagatcca
    14041 gcaggttgat ttttccatct ttcgtggtga cgttttcagg tgtgactaat tgttcagaca
    14101 tatgctattc cggcctcgtt attacacgtt atggcccctg gagggttgaa aaaagaaacg
    14161 ccccggtgag cggctgctcg tccgggggcg ctgcattgta caaattctgg cgcacggatg
    14221 ccacgtttgc acgcggcatt tacgaaatta ttaacgggtg cgcgggcaca cttcgccttc
    14281 gccaaagaaa taagcgattt cgcgagcggc agattcgacg gaatcagaac cgtgggtacc
    14341 gttttcggtc aggctgtcag cgtaatcagc gcgcagagta ccagccagtg cgtttgccgg
    14401 attggtcgcg cccagcagat cgcggtgacg ctgaacggcg ttttcacctt ccagcacgga
    14461 aaccacgatc gggccagagg tcatgaattc aaccagacca tcaaagaacg gttttccatc
    14521 gtgttcagca taaaagccac gtgcctgttc aacggtcagg tgcagcattt tggtgccgac
    14581 aattttgaac cctgcagctt caaagcgcgc aaagatatta ccaatgacgt tttttgctac
    14641 cgcgttcggt ttgatgatgg aaaaagtacg ttcaatagcc atttttacct ctgtaaattg
    14701 ttctgttgtt gtctgtacca gcgtacgaga tggcgcggat tataatgagc aacagggccg
    14761 ttgactattg atgaaggtaa cattttttta aaataaaatg aggtttagca acaatcaggg
    14821 acaaaacaga ctattgcatg acaaatttca ctgtcgcgat ttgccccacg tcatccatca
    14881 ccagcaattg ataatcacct ttatccgtca aatgcagggt cacgttgcgc ccgcgttcag
    14941 ttaacggttc gccattcaga aaccaccagc gttcacctgc ccctccactt gattgcaacg
    15001 gcaaagtcgc ttccgctgcg cccggtaaac gtttaataat cgcgccatcg cgaacgcctg
    15061 tcagctgcag cgggagctgg gcatagtgac cgtatggcgg gcaacttgtc gaggccggtg
    15121 gtaagcgcac agcgcgacgt tctgatgcgg gcagccaggg ttccagcggt agcggccaga
    15181 catttatcat ttcttgtcgc gcttgcgggc aatcagcggc aacacgtttg ccattttcat
    15241 ccagccagat ggggaaacga atgccattaa tgccttcctg ctccggcagt aatagagttg
    15301 gcggctgact cccgtccagc agccaggttg ccaggcggcg gcgacagtta ccgtcacctt
    15361 ccggcaaaga ctgtccgccc ggccagcaga taacgccacg agtgactgag ttcgggcgcg
    15421 ggtcttccgg cagattcgca ctgcgcgaca gtaagatatt attgacctga ttcaacaatg
    15481 gtacggcact ggcaaagcca aactgaccaa caacgggcgt gccgtccggt ctgccagtcc
    15541 agatcccaat gacatagcga gcgttaaccc caatcgccca ggcgtcacga tagccatagc
    15601 tggtgcccgt tttccatgcc agtggggcga cgcgcggcaa ggcactatcc ggcaagggtt
    15661 gcgcttcatc agccataatc cggcgaatga tccacgccgc ccccgacgac attaaaggcc
    15721 attcaagcag cggatcgtca ggctgtaagc gcaatttgcc tgccttgccg tggcgagcaa
    15781 acgcggtata cgctgccgcc atatcttcca gttttgcacc agcgccgccg agaatgagtg
    15841 aaagattcgg cgcagcaccg ttgggcaaat ataacggcaa tccaacattg cgtaactttg
    15901 ccgcaaaccg tttcggtcca taggcttcca gcacctgcac agcaggtaag ttcagcgagc
    15961 gcaccagcgc ctcgctcatg ctgatcgggc catgaaaacc gctatcaaag ttacctggtc
    16021 gataatcacc ggtgcgccgg gggacgtctt gcagcagtga tgccgggtgg atcaagcctt
    16081 catccagcgc cagaccataa acaaacggtt tgagcaccga tcctggcgat cggatcgcat
    16141 tgaccatatc aacatgacca aagcgtgaat catcgttgag atcaaccgat cccacccagc
    16201 cgcgaacacg catatcggta tgatcaacca cgatcatcgc cagtgagctg cgcggtggca
    16261 accgcccttt ccagttttgc gccagttctt ccagacgtcg ttgaagaccg gcatccagcg
    16321 tagtaacgat tttgtcgctt ttgcttttac cgagcatcat gcgcgaaaac agcggtgcca
    16381 gttgcggcat ttgtcggggt gccagccaga tgggttcttc ccttgactct tttacctgct
    16441 cacgggacca cacaccttgc acggccatcc gttcgagcac tttattacgc gcggcttcgg
    16501 cacgctccgg ccaacgatcc gggcgcagtc ggctgggcgc ttgcggtaac accgccagca
    16561 ttgccgcctc ggaatagctt aaattcgcag gcgattttcc gagataagcc caacttgccg
    16621 caccgatccc ctgcaacgta ccgccaaacg gagcgcggtt aagatacaag atcagaattt
    16681 cacgcttaga caaatgccac tccagttgca acgcgcgcca gagctggaga attttgccac
    16741 caaatgtttt ggggtgagga tcaagcagac gagcaacctg catagtgagc gtgctgccac
    16801 cagaaataac ccgtcccgaa gtgagatctt gccatgctgc gcgcgccacc gagaatggat
    16861 tcacccccgg atgcttccag aaccagcgat cttcataatt gatcagcgct tcaaggtaac
    16921 gtggagacac atcttcgatt gttaccggat aacgccagat gccatcagca tcggcgaagc
    16981 gccagagcgg cgtaccatcc tgcgccacga ccactcgtgc gggattgact tcatgcagcg
    17041 gtagaggcca gagtttatct gctccccacg ctgcaagaaa aaggagaaat ggcacagctg
    17101 ccagcgttat ccagcagccg cgtttggtta acaagcgaag catttacggt cggacaatca
    17161 gcagatcttc agccgcgccg gtcgcccgcc attggggaac atacattgat tccaccatcg
    17221 gttgcggtac ctgatacgtt cccggcgtca ccgcccgcgc cagatacacc agcgttaccg
    17281 gttggtattc atcaacggca accgccgcca caaagcgatc gtcacggaac tcaatgtgct
    17341 taatgctcgc ctgctgcatc tggttcagta agttttgcac ttcgtcacca ctttgctcca
    17401 ggctggcgct accgttcgcc agattctgat tttccagttc cagacccgca ggcagcagat
    17461 ccacgactaa cgcatccggc acgctgttac tggcttttac ctgcaaccag accagcacca
    17521 gatcgccgct acgtaacgag tccagcgatt tgctcttacc atcagtacca agaatatgac
    17581 gctcgatttg cagcacattg ttcgccggta aaggtgcgga ttgcggataa ccgctggcat
    17641 ccatacgcaa ccataacggc tgatcgccac tgtttctcac ctgcaaggtg gcaagttgat
    17701 cgctattcag attgctgttt agcgttttct cacctgtcag cggctcagct gagaaagagg
    17761 tttgcgcctg ccatttaccc ggtaaatcct gaatcgtacg ggcagccagg aacaacgcgt
    17821 tactttcctg cgtcgatagc cagcgttcac caaacgcctg ctgcgaaagt gtgttcagca
    17881 aagtgtactg ctcatcgggt agcagtttat tttcttccag caaggagagc attaacgcgt
    17941 tgtcgcgcag tggactaccg taatcaccca gccatatccg ctcatcacta tttctcggcg
    18001 ttttcagcgc cagcacaatc gcttcttcac cacgcgttgc atcacccatg gttttcagcg
    18061 caatgccaag ttgcagcagc ggtaaaccag aagcggcatc tgcacgatgc tcccagattt
    18121 cacgcagcgc acccagtggt gctttttgct gacgggccag caccagcgca gcgtaagact
    18181 gaacggcgaa tttactggct ttgagattat ccgcgtacgg gatcgacatc atgcccggat
    18241 cttgtaaata gcgcagcaga cgctcattac cccggttaat ggcgtctgtc ggcacgctgt
    18301 aaccctgttc gcctgcgcgg accaggaaat ccatcacgta agccgtcagc cagtactctt
    18361 cgtcaccgtt tttatcccac agcgcaaagc cgccgttatc acgttgcatt tgcagcaaac
    18421 gggaaatgcc gatatcgacc gatgcacggc gtttctcatc actgtcgcct ttgatgccca
    18481 acgcctgcag ttgggctgcg ttggtataaa gtgacgggaa caggccgctg gcggtttgct
    18541 caagacagcc gtacggatac gcttttaact ctttgatata acgtgcaatg ttcagcggtg
    18601 gtttgccgct caacaacaat tgcccttcca gcgtaacagg cgagaagttt tgcaatccat
    18661 ccgccggaat cgcccatgtc tcaccaggct gtaacgccgt accgtaatta accgtttgtg
    18721 ccgggaacgc cggacggacg ccgattttcc actgcttatg ctgatcggca acggtttcac
    18781 ccggtaacgc taacccgcta atggtggcct gaatttctcc atcgccataa cccggcaatg
    18841 ctcgcaccgg gataaacagc gtagtacgca cacctggcgc taattcaacg gctgcgggtg
    18901 aatcgctgac cagttcaagc aaaccactgg cggtcagggc aacgttcagt ttttgcggtt
    18961 tatcggtaag attagtgata tccagcgtca gacgcgaggt atcgccactc gccataaagc
    19021 gcggcatgtt cagttcagca atcaccggtg cggcaactat cactttgctt tcgttgctac
    19081 cgaaatcatc tgccgtccag gcttgcgcca tgacgcgcaa ttcaccgtta aaatcgccaa
    19141 tcggcagtgt aaccgagcct tcgccctgtt cgttgagcgt taccggcagc gcctgctgcg
    19201 cgacaatatt gacgtgattg accagcggtt taccaccacg tttcagctca tcaccatcgc
    19261 caccgaaacg cagagctgcc agacgcccct gaccttcaat aacctgaccg taaatatcgt
    19321 aaatgtctgc gccatagcgt ttctgaccaa agaacgcctg ccacggatct ggcgtgacgt
    19381 agtcagtaat attcagcaca ccactatcaa cggcagacac cagcacattc acctgtttag
    19441 gcttctcgcc atttttagtg ctggctttaa ttttcacggt taatggttga ttgggacgca
    19501 tttttgctgg tgtttccagc gccagatcga ggcgacggtt ttcatcgcca agcggcagat
    19561 gcaacacacc aaccgcgcgt tttggcgtcg cggagcgaga tttatcgcta ggacgtacca
    19621 ccagcgtact taaatacaga tcatgacgat tccaggtttt atcgaccgga atcgtcagat
    19681 ccagcccttg agccggaaca tcaatctctt gccaccacag cggcccttca ctggactcga
    19741 ccatcgcata acctttaccc gccgttggcg cggcgatatg caacttaatg gtgtcgccag
    19801 ggcgataact ggctttatcc agtttcagcg tgacacggtc gggtcgcact gcgccgctac
    19861 cgtcgctgtt gtcctgccaa ctatagccag cccagaaacg aacactactg accgcttcat
    19921 tcggcgcttt gacttccaga cgataagcgc cccactctac cggaaaactg accttgccgg
    19981 tttcgtccgc tttcagatcc agagtttgtt cattttcgat cagatctttt tgatcaaact
    20041 gtgactgcca gccttcatct tctgaccagt tccagtagta atcgcggcgt tcgcgaatca
    20101 ggcgcacctg caagcccgac acggcttttt tcacgccttg tgcatcgcta taaacgatgt
    20161 caaaagcggc gttactgcct tcatcaacaa tgggctgttt caccgtgctg tcagtgcgat
    20221 aatcgtaaac cgatttcaag gcgaactgcg gacggagccc cggcaatgca ttggcaggcc
    20281 agatagcctg ctcagcgcgg cgcgtcaccg ggcgaccgcc cgattccagc aaactgccct
    20341 ggaaaataac ctgcaatggg gaatgcgttt ccttccactg gctttctgta gaaacttcgc
    20401 cgcgcccttt atcatccagc gtcaactgaa cttcatccag cgtgcgggaa aggttttcgg
    20461 cagctatatc gccgaattca aaaccaggta aggctgacac agcctcacgc agtgggcgca
    20521 ggaaaagttg cccttgcaaa gtattaccat tagcaggcgc accgtacagg tagtatccca
    20581 ccacggagaa tttcacttca tcattcggcg taagcggggt tttctcaccg gtcagattca
    20641 gcgccatacg ctctggcata aaatcttcga cgtggaaatc ccacatccgg tactggttat
    20701 cgcccgtgtt ggcgcgaata tgccacatac cggttgccgc attgctatcg agtggccagg
    20761 taaagtggta gaggccattc tccggctgac taacgacgct cctgagtacc tgcccgtcag
    20821 gtttaatcac gtctaacttg atgggttgat cgggcaatgc tttaccgtct gcatcgcgca
    20881 gtaaaccatt gaggattacc gtttcacctg ggcgataaag atcgcgtggg ccaaacatga
    20941 aaaactgttt gctatagcct ggcgcgccag caatgttaaa ttctgctaag tccagcgccg
    21001 gaagttttaa atcgagtagc gttgtctgac cgtctttacg tgccagcagt aatgccgcat
    21061 ttttatcatt ttccagctgc acatgcccct gagcgtcact ggttgcctga gtcagagtct
    21121 gccctttctc atttaataaa gagacttcaa ttccttgctg ggccgcgccg ttttccagac
    21181 tttgggtaaa gatatccaga cgattgtgat aacggtgagc tgaaacgccg atatcactta
    21241 acgtaaacag cgtcgcggga ttactgtaat cgtaacgtcc agcctgattc atcacagcca
    21301 gatacacgcc cgcctgctga agcggtttga tatcgcccag cggcagcaat aatttttcac
    21361 gggtgttacg cgcaggattg agatcaaacc gtccggtgta gaccagatcc gccatctgca
    21421 gcagtttgtc tgactgccag ttcgccagcg aattgcggta ttcccattgg ctaatgaatg
    21481 ctggcagaga ttctggctta acgcggaaga agttaacatc gacattatta acgttgagcg
    21541 ccattaccgg cagcccttca acgactttgc caggcagcag cgaaccacgg ctggcaaaac
    21601 cgacgctggg ttggatgtcg cgggtagtta tagttttttc gtaatcttta ctgaaggttg
    21661 cgttgttgag tgctttgacc tccttgccaa tagtaacgat caaatcacgt ttcggttcga
    21721 ggtggcgtaa acgtagctct ttaagattat ctgacagctc ccaggcacca tccactttgc
    21781 cgcttttttt atcgacgaca tgaataacgc gtgagaaatc ctgatccggg tcgagaggga
    21841 tggagaacgt cagcaccagc gtggctgcac catcaagttg gacttcagag agatccagca
    21901 atgtcagcac cttaccggca ctctgttggg ccagtttttg tctctccggc acggagagtt
    21961 ttgctgaact cgcgttttct gaagaggccg ctttagtaac ttcagaaggc gcatcttttt
    22021 tcaccgctgt tggcgcgtta tcgttgttgt cgcaccctgc cagcgccagc attagcatgc
    22081 aggcggctac gcgtaacttt ttcatttttc atccctgcca caatggcccg ttagcaacgt
    22141 cgaataatta ttatgcgtga gaatttacgt tatgtaattc agta
//
LOCUS       DAJTMC010000060        21372 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.060, whole genome shotgun sequence.
ACCESSION   DAJTMC010000060 DAJTMC010000000
VERSION     DAJTMC010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21372
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.060"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            126..746
                     /locus_tag="OO130_001981"
     CDS             126..746
                     /locus_tag="OO130_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000654791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7726 family protein"
                     /protein_id="HCR5365408.1"
                     /translation="MKDQDVRFAVHHKLLKESHLDPDCLVVDEFSISLGASRADIAVI
                     NGVIHGYELKSEYDSLERLPLQIKHYSSVMDKVTLVVAEKHLEGALKLIPGWWGVKTV
                     SVGPKGAILIKHMRGEKLNRNHDTLMLAQLLWKDECIDVLERWGYSKGIKSKPRFELW
                     NIIAENIPIANLRLEVRTALKKRVGWKVKAWQAESAPTNKAVSRLT"
     gene            complement(688..1755)
                     /locus_tag="OO130_001982"
     CDS             complement(688..1755)
                     /locus_tag="OO130_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001383880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta family protein"
                     /protein_id="HCR5365409.1"
                     /translation="MSQHQYYPQLKWKPAEYESLMLLDQTTLSGFTPIITIPDIDWDY
                     ENECYKKSLSSYLSDFGINLAASWKANRPVLLDVKYLDKHGSSRHHPLDMCIQDARVN
                     GKEIIPVVSPAYSTNYIHAVQRNLINGLAISITPQTWHQFTSLVNHLNIHPSLIDVII
                     DFGDIQNATDSLKQQALSMVNTLSGQAPWRNLILSSTAYPASQAGIPQHQVHHIPRHE
                     YDLWMYVVQNFSNGRTPSFSDYPTASSTITSVDPRFMSQYVSVRYSNDTSWIFVKGTA
                     VKGNGWGQTKNLCTTLVSSPEYQVFGSKFSWGDDYIYQRSLGANKSGGSKEWRKVAHT
                     HHITLVVRQLYWLAQTQPAKP"
     gene            2168..2713
                     /locus_tag="OO130_001983"
     CDS             2168..2713
                     /locus_tag="OO130_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HCR5365410.1"
                     /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
                     AVIKWYAERDAEIENEKLRREVEELRQASEADLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSG"
     gene            2688..4613
                     /locus_tag="OO130_001984"
     CDS             2688..4613
                     /locus_tag="OO130_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HCR5365411.1"
                     /translation="MNISNSQVNRLRHFVRAGLRSLFRPEPQTAVEWADANYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL
                     IWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGKKHRDNTLTMKRFTNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDEDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDKETPFGLKWTPDDPSSVFYLCEH
                     NACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFT
                     TWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAERKEHYSAPVPDR
                     VAYLTAGIDSQLDRYEMRVWGWGPGEESWLIDRQIIMGRHDDEQTLLRVDEAINKTYT
                     RRNGAEMSISRICWDTGGIDPTIVYERSKKHGLFRVIPIKGASVYGKPVASMPRKRNK
                     NGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVHFPNNPDIFDLTEAQQLTAEEQV
                     EKWVDGRKKILWDSKKRRNEALDCFVYALAALRISISRWQLDLSALLASLQEEDGAAT
                     NKKTLADYARALSGEDE"
     gene            4610..4816
                     /locus_tag="OO130_001985"
     CDS             4610..4816
                     /locus_tag="OO130_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family protein"
                     /protein_id="HCR5365412.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTATSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
     gene            4813..6414
                     /locus_tag="OO130_001986"
     CDS             4813..6414
                     /locus_tag="OO130_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCR5365413.1"
                     /translation="MKTSTIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPSES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEE
                     EARAFSREVEAAWKEFAEDDCCCIDVERKRTFTMMIREGVAMHAFNGELFVQATWDTS
                     SSRLFRTQFRMVSPKRISNPNNTGDSRNCRAGVQINDSGAALGYYVSEDGYPGWMPQK
                     WTWIPRELPGGRASFIHVFEPVEDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKA
                     MYAATIESELDTQSAMDFILGANSQEQRERLTGWIGEIAAYYAAAPVRLGGAKVPHLM
                     PGDSLNLQTAQDTDNGYSVFEQSLLRYIAAGLGVSYEQLSRNYAQMSYSTARASANES
                     WAYFMGRRKFVASRQASQMFLCWLEEAIVRRVVTLPSKARFSFQEARSAWGNCDWIGS
                     GRMAIDGLKEVQEAVMLIEAGLSTYEKECAKRGDDYQEIFAQQVRETMERRAAGLKPP
                     AWAAAAFESGLRQSTEEEKSDSRAA"
     gene            6395..7714
                     /locus_tag="OO130_001987"
     CDS             6395..7714
                     /locus_tag="OO130_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HCR5365414.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISRLT
                     DAVSGDSLTAQEALATLALSGDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAALEKQGVEITLIYSGSHK
                     VDGNPYSHLPDDVRETLQSRMDATRQMFAQKVSAYTGLSVQAVLDTEAAVYSGQEAID
                     AGLADELVNSTDAITVMRDALDARKSRLSGGRMTKETQSTTVSATASQADVTDVVPAT
                     EGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQARVLAETPGMTVKTAR
                     RILAAAPQSAQARSDTALDRLMQGAPAPLAAGNPASDAVNDLLNTPV"
     gene            7724..8056
                     /locus_tag="OO130_001988"
     CDS             7724..8056
                     /locus_tag="OO130_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HCR5365415.1"
                     /translation="MTSKETFTHYQPQGNSDPAHTATAPGGLSAKAPAMTPLMLDTSS
                     RKLVAWDGTTDSAAVGILAVAADQTSTTLTFYKSGTFRYEDVLWPEAASDETKKRTAF
                     AGTAISIV"
     gene            8112..9137
                     /locus_tag="OO130_001989"
     CDS             8112..9137
                     /locus_tag="OO130_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HCR5365416.1"
                     /translation="MSMYTTAQLLAANEQKFKFDPLFLRLFFRESYPFTTEKVYLSQI
                     PGLVNMALYVSPIVSGEVIRSRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLA
                     DPAYRRRRIIMQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSAAN
                     NITQSGSTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVREKL
                     DTRRGSHSELETAVKDLGKAVSYKGMYGDVAIVVYSGQYVENGVKKNFLPDNTMVLGN
                     TQARGLRTYGCIQDADAQREGINASARYPKNWVTTGDPAREFTMIQSAPLMLLADPDE
                     FVSVQLA"
     gene            9179..9574
                     /locus_tag="OO130_001990"
     CDS             9179..9574
                     /locus_tag="OO130_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HCR5365417.1"
                     /translation="MTKDELIVRLRSLGEQLNRDVSLTGTKEELALRVAELEEELDDT
                     DDAAGQDTPLRPENALTGHENEVVSAQTDTVTDAAALVTVVALVTLHTDALHATRDEP
                     VAFVLPETAFRVSAGVAAEMTERGLARMQ"
     gene            9586..9939
                     /locus_tag="OO130_001991"
     CDS             9586..9939
                     /locus_tag="OO130_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HCR5365418.1"
                     /translation="MADFDNLFDAAIARADETIRGYMGTSATMTSGEQSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDEVRQLRRGDTLTIGEENFWIDRISPDDGGSCH
                     LWLGRGVPPAVNRRR"
     gene            9951..10529
                     /locus_tag="OO130_001992"
     CDS             9951..10529
                     /locus_tag="OO130_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5365419.1"
                     /translation="MAIKGLEQAVENLSRISKTAVPGAAAMAINRVASSAISQSASQV
                     ARETKVRRKLVKERARLKRATVKNPQARIKVNRGDLPVIKLGNARVVLSRRRRRKKGQ
                     RSSLKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            10526..10921
                     /locus_tag="OO130_001993"
     CDS             10526..10921
                     /locus_tag="OO130_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5365420.1"
                     /translation="MKHTELRAAVLDALEKHDTGATLFDGRPAVFDEEDFPAIAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDAWMESRIYPVMSDIPALSDLITSM
                     VASGYDYRRDDDAGLWSSADLTYVITYEM"
     gene            10929..11669
                     /locus_tag="OO130_001994"
     CDS             10929..11669
                     /locus_tag="OO130_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001605275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5365421.1"
                     /translation="MPVPNPTMPVKGAGTTLWVYKGSGDPYANPLSDVDWSRLAKVKD
                     LTPGELTAESYDDSYLDDKDADWTATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     ATGMTVTPASTSVVKGQSTTLTVAFQPEGATDKSFRAVSADKTKATVSVSGMTITVKG
                     VAAGKVNIPVVSGNGELAVVAEITVTDS"
     gene            11685..12107
                     /locus_tag="OO130_001995"
     CDS             11685..12107
                     /locus_tag="OO130_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HCR5365422.1"
                     /translation="MFLKTESFEYNGVSVTLSELSALQRIEHLALLKRQAEQAGSSLN
                     RQVSVEDLVRTGAFLVAMSLWHSHPQKTKMPSMNEAVKQIEQEVLTTWPTEAIAQAEN
                     VVMRLSGMSEFVVNDAPEQADDAGPAEPVSAGKCSTVS"
     gene            12089..12523
                     /locus_tag="OO130_001996"
     CDS             12089..12523
                     /locus_tag="OO130_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HCR5365423.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSIHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPDMHPLDFSLLNRREADEEPEDDVLMQKAAGLAG
                     GVRFGPDGNEVIPASPDVADMTEDDVMLMTVSEGIAGGVRYG"
     gene            12516..15077
                     /locus_tag="OO130_001997"
     CDS             12516..15077
                     /locus_tag="OO130_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR5365424.1"
                     /translation="MAEPVGDLVVDLSLDAARFDEQMARVRRHFSGTESDAKKTAAVV
                     EQSMNRQALAAQKVGISVGQYKAAMRMLPAQFTDVATQLAGGQSPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGATSLAVATGALAYAWYQGNSTLSDFNKTLVLSGNQA
                     GLTADRMLVLSRAGQAAGLTFNQTSESLSALVKAGVSGEAQIASISQSVARFSSASGV
                     EVDKVAEAFGKLTTDPTSGLTAMARQFHNVTAEQIAYVAQLQRSGDEAGALQAANEAA
                     TKGFDDQTRRLKENMGTLETWADRTARAFKSMWDAVLDIGRPDTAQEMLIKAEAAFKK
                     ADDIWNLRKDDYFVNDEARARYWDDREKARLALEAARKKAEQQTQQDKNAQQQSDTEA
                     SQLKYTEEAQKAYERLQTPLEKYTARQEELNKALKDGKILQADYNTLMAAAKKDYEAT
                     LKKPKQSGVKVSAGDRQEDSAHAALLTLQAELRTLEKHAGANEKISQQRRDLWKAESQ
                     FAVLEEAAQCRQLSAQEKSLLAHKDETLEYKRQLAALGDKVTYQERLNALAQQADKFA
                     QQQRAKRAAIDAKSRGLTDRQAEREATEQRLKEQYGDNPLALNNVMSEQKKTWAAEDQ
                     LRGNWMAGLKSGWSEWEESATDSMSQVKSAATQTFDGIAQNMAAMLTGSEQNWRSFTR
                     SVLSMMTEILLKQAMVGIVGSIGSAIGGAVGGGASASGGTAIQAAAAKFHFATGGFTG
                     TGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYATGGYVGTPGSMADSRSQAS
                     GTFEQNNHVVINNDGTNGQIGPAALKAVYDMARKGARDEIQTQMRDGGLFSGGGR"
     gene            15074..15403
                     /locus_tag="OO130_001998"
     CDS             15074..15403
                     /locus_tag="OO130_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5365425.1"
                     /translation="MKTFRWKVKPGMDVASVPSVRKVRFGDGYSQRAPAGLNANLKTY
                     SVTLSVPREEATVLESFLEEHGGWKSFLWTPPYEWRQIKVTCAKWSSRVSMLRVEFSA
                     EFEQVVN"
     gene            15403..16101
                     /locus_tag="OO130_001999"
     CDS             15403..16101
                     /locus_tag="OO130_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HCR5365426.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQPYPIQGSGFELNGKGTSTRPTLTVSNLYGMVTGMAEDMQSLVGGT
                     VVRRKVYAHFLDAVNFVNGNSYADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYSGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV
                     GNFGGFLSINKLSQ"
     gene            16107..16850
                     /locus_tag="OO130_002000"
     CDS             16107..16850
                     /locus_tag="OO130_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5365427.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVSTPEGERYFPCVNISGEPE
                     AYFRMSPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGTIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFYREDDWWRNGQNLYLDNLEAT
                     GLYQVPLSAAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHREWHASAFTGICNDLAAASTFV"
     gene            16787..17419
                     /locus_tag="OO130_002001"
     CDS             16787..17419
                     /locus_tag="OO130_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HCR5365428.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRALATQLPAFRQKLSD
                     GWYQVRIAGRDVSTSGLTAQLHETLPDGAVIHIVPRVAGAKSGGVFQIVLGAAAIAGS
                     FFTAGATLAAWGAAIGAGGMTGILFSLGASMVLGGVAQMLAPKDRTPRTQTTDNGKQN
                     TCFSSLDNMVAQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            17480..20878
                     /locus_tag="OO130_002002"
     CDS             17480..20878
                     /locus_tag="OO130_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HCR5365429.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFSIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQKVSRNYHLRGRVLQVPSNYNPQTRQYSGIWDGTFKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAWLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPDNGWETATELVEDTQAILRYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISIGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPDGVAGYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVAADDGSERLVSTARTAETTYRFTQ
                     LALGRYTLTVRAVNAWGQQGDPASVSFRIAAPVAPSRIELTPGYFQITATPHLAVYDP
                     TVQFEFWFSETRITDIRQVETTARYLGAGLYWIAASINIKPGHDYYFYIRSVNTVGKS
                     AFVEAVGRASDDAEGYLDFFKGKITESHLGKELLEKVDLTEDNASRLDEFSKEWKDAN
                     DKWNAMWGVKIEQTKDGKHYVAGIGLSMEDTEEGKLSQFLVAANRIAFIDPANGNETP
                     MFVAQGNQIFMNDVFLKRLTAPTITSGGNPPAFSLTPDGKLTAKNADISGNVNANSGT
                     LNNVTINENCQIKGKLSANQIEGDIVKTVSKSFPRTNSYASGTITVRISDDQKFDRQV
                     MIPPVLFRGGKHENFNSNNQQSYWYSTCRLRVTRNGQEIFNQSTTDAQGVFSSVIDMP
                     AGQGTLTLTFTVSSSGANNWTPTTSISDLLVVVMKKSTAGISIS"
     gene            20945..>21372
                     /locus_tag="OO130_002003"
     CDS             20945..>21372
                     /locus_tag="OO130_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HCR5365430.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHQSTLSAGYLHARTNVPG
                     SDDLNGINVKYRYEFTDTLGMVTSFSYAGDKNRQITRYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKKH"
BASE COUNT         5032 a         5282 c         6521 g         4537 t
ORIGIN      
        1 atagtcgtat ggctatgaat cttgttgctg cagataagca ttttgtgatt gaagtggtca
       61 aatctctcaa atgcagtaaa atgcagtgcg ctataattca ataacggagg gagtaaggag
      121 aagtcatgaa agatcaagat gttaggttcg cggtgcatca taagcttttg aaagaatcgc
      181 atttagatcc agactgcctt gtggtcgatg aattttccat atcccttggc gccagtagag
      241 cagacattgc tgtaataaat ggtgttatac acgggtacga gctcaaaagt gaatatgact
      301 ctttggagcg tttgcctctt caaatcaagc attattcttc tgtaatggac aaggttactc
      361 ttgtcgtagc tgagaaacat cttgagggag cattaaagtt aatcccaggt tggtggggcg
      421 ttaaaacggt ttctgttggg ccaaaaggcg ccattcttat aaagcacatg cgtggagaaa
      481 agcttaatcg aaaccatgac acattgatgc tcgctcaatt gctttggaaa gatgaatgta
      541 tcgacgtact tgaacgatgg ggctattcca aaggaatcaa aagcaagccc cgatttgagt
      601 tatggaatat tattgcggaa aatattccaa tagcgaatct caggcttgaa gtcagaacag
      661 ccttaaagaa acgcgtaggc tggaaagtta aggcttggca ggctgagtct gcgccaacca
      721 ataaagctgt ctcacgacta acgtaatatg gtgcgtatgt gcaactttac gccattcttt
      781 agagccgcca gatttgttag cgcctaatga tctttggtaa atgtaatcat ccccccaact
      841 aaatttggaa ccaaagactt gatactctgg cgaactaaca agggtagtac ataagttttt
      901 agtttggccc catccatttc ctttaactgc ggtacctttt acaaagatcc atgaggtatc
      961 gttcgaatat ctcactgaga catactgaga catgaagcgt gggtctacgc tcgtaatggt
     1021 agagctagcg gtgggataat cactaaaact tggcgttctt ccattgctaa aattctgtac
     1081 tacgtacatc caaagatcgt attcatggcg cggaatatga tgaacttgat gttgcggtat
     1141 ccctgcctgt gatgctgggt atgcggttga agataaaatc aagtttctcc acggagcttg
     1201 gcctgataat gtgttgacca tgcttaatgc ttgttgtttt aaactatcag ttgcgttttg
     1261 aatatctcca aaatcaatga ttacatcaat taaactagga tgaatattta agtggttaac
     1321 cagacttgtg aattggtgcc atgtctgggg ggtgatagat atagcgagcc cattgattaa
     1381 gttgcgttga acagcatgta tatagtttgt tgaatatgcg ggagaaacaa cagggataat
     1441 ttccttacca tttactctag catcttggat acacatatct agaggatgat ggcggctcga
     1501 accatgttta tctaaatatt taacatccag caaaacagga cgattggctt tccaggatgc
     1561 cgcaaggtta ataccgaagt cagataagta agaactcaaa ctcttcttgt agcattcgtt
     1621 ttcataatcc cagtctatgt ctggaatggt aatgatcgga gtaaaaccag agagcgtagt
     1681 ttgatctaaa agcatcagag attcatattc agcaggcttc catttcagct gtggataata
     1741 ttgatgttga ctcattaaaa ctccttaaaa tctttactta tcctaagtat agtgttatgg
     1801 cgtaacgcca attgttttta tacacttgaa aacattggga aaagttgagc tatatctaaa
     1861 tttacaaaat tggatgttat agaaatcgag gtttcaacta ggcatggaac tgcaaaattt
     1921 ttaatgcctg cacgcgaaag tcgttggcgg gtcctttccg gtgatccaga ccgttacggg
     1981 gcggcgacct cgcgggtttt cgctatttat gaaaattttc cggtttaagg cgtttccgtt
     2041 cttcttcgtc gtaacttaat gtttttattt aaaatacccc ctgaaaagaa aggaaacgac
     2101 aggtgctgaa aacgaacttt tgggcctttg tcgtttcctt tctctgtttt tggccgtgga
     2161 atgaacaatg gaagtcaaca aaaagcagct ggctgacatt ttcggtgcga gtatccgtac
     2221 cattcagaac tggcaggaac agggaatgcc cgttctgcga ggcggtggca agggtaatga
     2281 ggtgctttat gactctgccg ccgttataaa atggtatgcc gaaagggatg ctgaaattga
     2341 gaacgaaaag ctgcgccggg aggttgaaga actgcgccag gccagcgagg cagatctcca
     2401 gccagggact attgagtacg aacgccatcg acttacgcgt gcgcaggccg acgcacagga
     2461 actgaagaat gccagagact cagctgaagt ggtggaaacc gcattctgta ctttcgtgct
     2521 gtcgcggatc gcaggtgaaa ttgccagtat tctcgacggg atccccctgt cggtgcagcg
     2581 gcgttttccg gaactggaaa accgacatgt tgatttcctg aaacgggata tcatcaaagc
     2641 catgaacaaa gcagccgcgc tggatgaact gataccgggg ttgctgagtg aatatatcga
     2701 acagtcaggt taacaggctg cggcattttg tccgcgccgg gcttcgctca ctgttcaggc
     2761 cggagccaca gaccgccgtt gaatgggcgg atgctaatta ctatctcccg aaagaatccg
     2821 cataccagga agggcgctgg gaaacactgc cctttcagcg ggccatcatg aatgcgatgg
     2881 gcagcgacta catccgtgag gtgaatgtgg tgaagtctgc ccgtgtcggt tattccaaaa
     2941 tgctgctggg tgtttatgcc tactttatag agcataagca gcgcaacacc cttatctggt
     3001 tgccgacgga tggtgatgcc gagaacttta tgaaaaccca cgttgagccg actattcgtg
     3061 atattccgtc gctgctggcg ctggccccgt ggtatggcaa aaagcaccgg gataacacgc
     3121 tcaccatgaa gcgtttcact aatgggcgtg gcttctggtg cctgggcggt aaagcggcaa
     3181 aaaactaccg tgaaaagtcg gtggatgtgg cgggttatga tgaacttgct gcctttgatg
     3241 aggatattga acaggaaggc tctccgacgt tccttggcga caaacgtatt gaaggctcgg
     3301 tctggccaaa gtccatccgt ggctccacgc ccaaagtgag aggcacctgc cagattgagc
     3361 gtgcagccag tgaatccccg cattttatgc gttttcatgt tgcctgcccg cactgcgggg
     3421 aggagcagta tcttaaattt ggcgacaaag agacgccgtt tggcctcaaa tggacgccgg
     3481 atgacccctc cagcgtgttt tatctctgcg agcataatgc ctgcgtcatc cgccagcagg
     3541 agctggactt tactgatgcc cgttatatct gcgaaaagac cgggatctgg acccgtgatg
     3601 gcattctctg gttttcgtca tccggtgaag agattgagcc acctgacagt gtgacctttc
     3661 acatctggac agcgtacagc ccgttcacca cctgggtgca gattgtcaaa gactggatga
     3721 aaacgaaagg ggatacggga aaacgtaaaa ccttcgtaaa caccacgctc ggtgagacgt
     3781 gggaggcgaa aattggcgaa cgtccggatg ctgaagtgat ggcagagcgg aaagagcatt
     3841 attcagcgcc cgttcctgac cgtgtggctt acctgaccgc cggtatcgac tcccagctgg
     3901 accgctacga aatgcgcgta tggggatggg ggccgggtga ggaaagctgg ctgattgacc
     3961 ggcagattat tatgggccgc cacgacgatg aacagacgct gctgcgtgtg gatgaggcca
     4021 tcaataaaac ctatacccgc cggaatggtg cagaaatgtc gatatcccgt atctgctggg
     4081 atactggcgg gattgacccg accattgtgt atgaacgctc gaaaaaacat gggctgttcc
     4141 gggtgatccc cattaaaggg gcatccgtct acggaaagcc ggtggccagc atgccacgta
     4201 agcgaaacaa aaacggggtt taccttaccg aaatcggtac ggataccgcg aaagagcaga
     4261 tttataaccg cttcacactg acgccggaag gggatgaacc gcttcccggt gccgttcact
     4321 tcccgaataa cccggatatt tttgatctga ccgaagcgca gcagctgact gctgaagagc
     4381 aggtcgaaaa atgggtggat ggcaggaaaa aaatactgtg ggacagcaaa aagcgacgca
     4441 atgaggcgct cgactgcttc gtttatgcgc tggcggcgct gcgcatcagt atttcccgct
     4501 ggcagctgga tctcagtgca ctgctggcga gcctgcagga agaggatggt gcagcaacca
     4561 acaagaaaac actggcagat tacgcccgtg ccttatccgg agaggatgaa tgacgcgaca
     4621 ggaagaactt gccgctgccc gtgcggcact gcatgacctg atgacaggaa aacgggtggc
     4681 aacggtacag aaagacggac ggagagtgga gtttacggcc acttccgtgt ctgacctgaa
     4741 aaaatacatt gcggagctgg aagtgcagac cggcatgaca cagcgacgca ggggacctgc
     4801 aggattttat gtatgaaaac gtccaccatt cccacccttc tggggccgga cggcatgaca
     4861 tcgctgcgtg aatatgccgg ttatcacggc ggtggcagcg gatttggagg gcagttgcgg
     4921 gcgtggaacc caccgagtga aagtgtggat gcagccctgc tgcccaactt tacccgtggc
     4981 aatgcccgcg cagacgatct ggtacgcaat aacggctatg ccgccaacgc catccagttg
     5041 catcaggatc atatcgtcgg gtcttttttc cggctcagtc atcgcccaag ctggcgctat
     5101 ctgggcatcg gggaggaaga agcccgtgcc ttttcccgcg aggttgaagc ggcatggaaa
     5161 gagtttgccg aggatgactg ctgctgcatt gacgttgagc gaaaacgcac gtttaccatg
     5221 atgattcggg aaggtgtggc catgcacgcc tttaacggtg aactgttcgt tcaggccacc
     5281 tgggatacca gttcgtcgcg gcttttccgg acacagttcc ggatggtcag cccgaagcgc
     5341 atcagcaacc cgaacaatac cggcgacagc cggaactgcc gtgccggtgt gcagattaat
     5401 gacagcggtg cggcgctggg atattacgtc agcgaggacg ggtatcctgg ctggatgccg
     5461 cagaaatgga catggatacc ccgtgagtta cccggcgggc gcgcctcgtt cattcacgtt
     5521 tttgaacccg tggaggacgg gcagactcgc ggtgcaaatg tgttttacag cgtgatggag
     5581 cagatgaaga tgctcgacac gctgcagaac acgcagctgc agagcgccat tgtgaaggcg
     5641 atgtatgccg ccaccattga gagtgagctg gatacgcagt cagcgatgga ttttattctg
     5701 ggcgcgaaca gtcaggagca gcgggaaagg ctgaccggct ggattggtga aattgccgcg
     5761 tattacgccg cagcgccggt ccggctggga ggcgcaaaag taccgcacct gatgccgggt
     5821 gactcactga acctgcagac ggctcaggat acggataacg gctactccgt gtttgagcag
     5881 tcactgctgc ggtatatcgc tgccgggctg ggtgtctcgt atgagcagct ttcccggaat
     5941 tacgcccaga tgagttactc cacggcacgg gccagtgcga acgagtcgtg ggcgtacttt
     6001 atggggcggc gaaaattcgt cgcatcccgt caggcgagcc agatgtttct gtgctggctg
     6061 gaagaggcca tcgttcgccg cgtggtgacg ttaccttcaa aagcgcgctt cagttttcag
     6121 gaagcccgca gtgcctgggg gaactgcgac tggataggct ccggtcgtat ggccatcgat
     6181 ggtctgaaag aagttcagga agcggtgatg ctgatagaag ccggactgag cacctacgag
     6241 aaagagtgcg cgaaacgcgg tgacgactat caggaaattt ttgcccagca ggtccgtgaa
     6301 acgatggagc gccgtgcagc cggtcttaaa ccgcccgcct gggcggctgc agcatttgaa
     6361 tccgggctgc gacaatcaac agaggaggag aagagtgaca gcagagctgc gtaatctccc
     6421 gcatattgcc agcatggcct ttaatgagcc gctgatgctt gaacccgcct atgcgcgggt
     6481 tttcttttgt gcgcttgcag gccagcttgg gatcagccgc ctgacggatg cggtgtccgg
     6541 cgacagcctg actgcccagg aggcactcgc gacgctggca ttatccggtg atgatgacgg
     6601 accacgacag gcccgcagtt atcaggtcat gaacggcatc gccgtgctgc cggtgtccgg
     6661 cacgctggtc agccggacgc gggcgctgca gccgtactcg gggatgaccg gttacaacgg
     6721 cattatcgcc cgtctgcaac aggctgccag cgatccgatg gtggacggca ttctgctcga
     6781 tatggacacg cccggcggga tggtggcggg ggcatttgac tgcgctgaca tcatcgcccg
     6841 tgtgcgtgac ataaaaccgg tatgggcgct tgccaacgac atgaactgca gtgcaggtca
     6901 gttgcttgcc agtgccgcct cccggcgtct ggtcacgcag accgcccgga caggctccat
     6961 cggcgtcatg atggctcaca gtaattacgg tgctgcgctg gagaaacagg gtgtggaaat
     7021 cacgctgatt tacagcggca gccataaggt ggatggcaac ccctacagcc atcttccgga
     7081 tgacgtccgg gagacactgc agtcccggat ggacgcaacc cgccagatgt ttgcgcagaa
     7141 ggtgtcggca tataccggcc tgtccgtgca ggctgtgctg gataccgagg ctgcagtgta
     7201 cagcggtcag gaggccattg atgccggact ggctgatgaa cttgttaaca gcaccgatgc
     7261 gatcaccgtc atgcgtgatg cactggatgc acgtaaatcc cgtctctcag gagggcgaat
     7321 gaccaaagag actcaatcaa caactgtttc agccactgct tcgcaggctg acgttactga
     7381 cgtggtgcca gcgacggagg gcgagaacgc cagcgcggcg cagccggacg tgaacgcgca
     7441 gatcaccgca gcggttgcgg cagaaaacag ccgcattatg gggatcctca actgtgagga
     7501 ggctcacgga cgcgaagaac aggcacgcgt gctggcagaa acccccggta tgaccgtgaa
     7561 aacggcccgc cgcattctgg ccgcagcacc acagagtgca caggcgcgca gtgacactgc
     7621 gctggatcgt ctgatgcagg gggcaccggc accgctggct gcaggtaacc cggcatctga
     7681 tgccgttaac gatttgctga acacaccagt gtaagggatg tttatgacga gcaaagaaac
     7741 ctttacccat taccagccgc agggcaacag tgacccggct cataccgcaa ccgcgcccgg
     7801 cggattgagt gcgaaagcgc ctgcaatgac cccgctgatg ctggacacct ccagccgtaa
     7861 gctggttgcg tgggatggca ccaccgacag tgctgccgtt ggcattcttg cggttgctgc
     7921 tgaccagacc agcaccacgc tgacgttcta caagtccggc acgttccgtt atgaggatgt
     7981 gctctggccg gaggctgcca gcgacgagac gaaaaaacgg accgcgtttg ccggaacggc
     8041 aatcagcatc gtttaacttt acccttcatc actaaaggcc gcctgtgcgg ctttttttac
     8101 gggatttttt tatgtcgatg tacacaaccg cccaactgct ggcggcaaat gagcagaaat
     8161 ttaagtttga tccgctgttt ctgcgtctct ttttccgtga gagctatccc ttcaccacgg
     8221 agaaagtcta tctctcacaa attccgggac tggtaaacat ggcgctgtac gtttcgccga
     8281 ttgtttccgg tgaggttatc cgttcccgtg gcggctccac ctctgaattt acgccgggat
     8341 atgtcaagcc gaagcatgaa gtgaatccgc agatgaccct gcgtcgcctg ccggatgaag
     8401 atccgcagaa tctggcggac ccggcttacc gccgccgtcg catcatcatg cagaacatgc
     8461 gtgacgaaga gctggccatt gctcaggtcg aagagatgca ggccgtttct gccgtgctca
     8521 agggcaaata caccatgacc ggtgaagcct tcgatccggt tgaggtggat atgggccgca
     8581 gtgcggcgaa caacatcacg cagtccggca gcacggagtg gagcaagcgt gacaagtcca
     8641 cgtatgaccc gaccgacgat atcgaagcct acgcgctgaa cgccagcggc gtggtgaata
     8701 tcatcgtgtt tgatccgaaa ggctgggcgc tgttccgttc cttcaaagcc gtcagggaga
     8761 agctggatac ccgtcgcggc tctcattccg agctggagac agcggtgaaa gacctgggta
     8821 aagcggtgtc ctataagggg atgtatggcg atgtggccat cgtcgtgtat tccggacagt
     8881 acgtggaaaa cggcgtcaaa aagaacttcc tgccggacaa cacgatggta ctggggaaca
     8941 ctcaggcacg cggtctgcgc acctatggct gcattcagga tgcggacgca cagcgcgaag
     9001 gcattaacgc ctctgcccgt tacccgaaaa actgggtgac caccggcgat ccggcgcgtg
     9061 agttcaccat gattcagtca gcaccgctga tgctgctggc tgaccctgat gagttcgtgt
     9121 ccgtacaact ggcgtaatcg tggcccttcg gggccatttt ctctctgtgg aggagtccat
     9181 gacgaaagat gaactgattg tccgtctccg gtcgctgggt gagcaactga accgtgatgt
     9241 cagcctgacg gggacgaaag aagaactggc gctccgtgtg gcagagctgg aagaggagct
     9301 tgatgacacg gatgacgctg ccggtcagga tacccctctc agaccggaaa atgcgctgac
     9361 cggacatgaa aatgaggtgg tatcagcaca gacggatacc gtgactgatg cggctgctct
     9421 ggtcacggtt gtggcactgg tgacgctgca taccgatgca cttcacgcca cgcgggatga
     9481 acctgtggca tttgtgctgc cggaaacggc gttccgtgtc tctgccggtg tggcagctga
     9541 aatgacagag cgcggcctgg ccagaatgca ataacgggag gcgctgtggc tgatttcgat
     9601 aacctgttcg atgctgccat tgcccgcgcc gatgaaacga tacgcgggta catgggaacg
     9661 tcagccacca tgacatccgg tgagcagtcc ggcgcagtaa tacgtggtgt ttttgatgac
     9721 cctgaaaata tcagctatgc cggacagggc gtacgcgttg aaggctccag cccgtccctg
     9781 tttgtccgga ctgatgaggt gcggcagctg cggcgcggcg acacgctgac catcggtgag
     9841 gaaaacttct ggatagaccg gatttcgccg gatgatggcg gaagctgtca tctctggctt
     9901 gggcggggcg taccgcctgc cgttaaccgt cgccgctgaa agggggatgt atggccataa
     9961 aaggtcttga gcaggccgtt gaaaacctca gccgtatcag taaaacggcg gtgcccggtg
    10021 ccgccgcaat ggccattaac cgcgttgctt catccgcgat atcgcagtcg gcgtcacagg
    10081 ttgctcgtga gacaaaggta cgccggaaac tggtaaagga aagggccagg ctgaaaaggg
    10141 ccacggtcaa aaatccgcag gccagaatca aagttaaccg gggggatttg cccgtaatca
    10201 agctgggtaa tgcgcgggtt gtcctttcgc gccgcaggcg tcgtaaaaag gggcagcgtt
    10261 catccctgaa aggtggcggc agcgtgcttg tggtgggaaa ccgtcgtatt cccggcgcgt
    10321 ttattcagca actgaaaaat ggccgctggc atgtcatgca gcgtgtggcc gggaaaaacc
    10381 gttaccccat tgatgtggtg aaaatcccga tggcggtgcc gctgaccacg gcgtttaaac
    10441 agaatattga acggatacgg cgtgaacgtc ttccgaaaga gctgggctat gcgctgcagc
    10501 atcaactgag aatggtaata aagcgatgaa acatactgaa ctccgtgcag ccgtactgga
    10561 tgcactggag aagcatgaca ccggggcgac gctttttgat ggtcgccccg ctgtttttga
    10621 tgaggaagat tttccggcaa ttgccgttta tctcaccggc gctgaataca cgggcgaaga
    10681 gctggacagc gatacctggc aggcggagct gcatattgaa gttttcctgc ctgctcaggt
    10741 gccggattca gagctggatg cgtggatgga gtcccggatt tatccggtga tgagcgatat
    10801 cccggcactg tcagatttga tcaccagtat ggtggccagt ggctatgact accggcgcga
    10861 cgatgatgcg ggcctgtgga gttcagccga tctgacttat gtcattacct atgaaatgtg
    10921 aggacgctat gcctgtacca aatcctacaa tgccggtgaa aggtgccggg accaccctgt
    10981 gggtttataa ggggagcggt gacccttatg cgaacccgct ttcagacgtt gactggtcgc
    11041 gtctggcaaa agttaaagac ctgacgcccg gcgaactgac cgctgagtcc tatgacgaca
    11101 gctatctcga tgataaagat gcagactgga ctgcgaccgg gcaggggcag aaatctgccg
    11161 gagataccag cttcacgctg gcgtggatgc ccggagagca ggggcagcag gcgctgctgg
    11221 cgtggtttaa tgaaggtgat acccgtgcct ataaaatccg cttcccgaac ggcacggtcg
    11281 atgtgttccg tggctgggtc agcagtatcg gtaaggcggt gacggcgaag gaagtgatta
    11341 cccgcacggt gaaggtcacc aatgtgggac gtccgtcgat ggcagaagat cgcagcacgg
    11401 taacagcggc aaccggcatg accgtgacgc ctgccagcac ttcggtggtg aaagggcaga
    11461 gcaccacgct gaccgtggca ttccagccgg aaggcgcaac cgacaagagc ttccgtgcgg
    11521 tgtctgcgga taaaacaaaa gccaccgtgt cggtcagtgg tatgaccatc accgtgaaag
    11581 gtgttgctgc aggcaaggtc aacattccgg ttgtatccgg taatggtgaa cttgctgtgg
    11641 ttgcagaaat caccgtcacc gacagttaat ccggagagtc agcgatgttc ctgaaaaccg
    11701 aatcatttga atataacggt gtgagcgtca cgctttctga actgtcagcc ctgcagcgaa
    11761 ttgagcatct cgccctgctg aaacgacagg cagaacaggc gggatccagt ctcaatcgac
    11821 aggtgagcgt ggaagatctc gtcagaaccg gtgcttttct ggtggcgatg tccctgtggc
    11881 atagccatcc gcagaagaca aagatgccgt ccatgaatga agccgttaaa caaattgagc
    11941 aggaagtgct taccacctgg cccacagagg caattgctca ggctgaaaat gtggtaatgc
    12001 gtctgtccgg tatgtctgag tttgttgtga atgatgcacc tgaacaggca gatgacgccg
    12061 ggccagcaga gcctgtttct gcgggaaagt gttcgacggt gagctgagtt ttgccctgaa
    12121 actggcgcgt gagatggggc gacccgactg gcgcgccatg cttgccggga tgtcatccac
    12181 ggagtatgcc gactggcacc gcttttacag tatccattat tttcatgatg ttctgctgga
    12241 tatgcacttt tccgggctga cgtacaccgt actcagcctg tttttcagcg atccggatat
    12301 gcatccgctg gatttcagtc tgctgaaccg gcgcgaggct gacgaagagc ctgaagatga
    12361 tgtgctgatg cagaaagcgg cagggcttgc cggaggcgtc cgctttggcc cggacgggaa
    12421 tgaagttatc cccgcttccc cggatgtggc ggacatgacg gaggatgacg taatgctgat
    12481 gacagtatca gaagggatcg caggaggagt ccggtatggc tgaaccggta ggcgatctgg
    12541 tcgttgattt gagtctggat gcggccagat ttgacgagca gatggccaga gtcaggcgtc
    12601 atttttccgg tacggaaagt gatgcgaaaa aaacagcggc agtcgttgaa cagtcgatga
    12661 accggcaggc gctggctgca cagaaagtgg gaatttccgt cgggcagtat aaagccgcca
    12721 tgcgtatgct gcctgcgcag ttcactgacg tggccacgca gcttgcaggc gggcaaagtc
    12781 cgtggctgat cctgctgcaa caggggggtc aggtgaagga ctccttcggc gggatgatcc
    12841 ccatgttccg ggggcttgcc ggtgcgatca ccctgccgat ggtgggggcc acctcgctgg
    12901 cggtggcgac cggagcgctg gcgtatgcct ggtatcaggg caactcaacc ctgtccgatt
    12961 tcaacaaaac gctggtcctt tccggcaatc aggcgggact gacggcagat cgtatgctgg
    13021 tcctgtccag agccgggcag gcggcagggc tgacgtttaa ccagaccagc gagtcactca
    13081 gcgcactggt taaggcgggg gtaagcggtg aggctcagat tgcgtccatc agccagagtg
    13141 tggcgcgttt ctcctctgca tccggcgtgg aggtggacaa ggtcgctgaa gccttcggga
    13201 agctgaccac agacccgacg tcggggctga cggcgatggc acgccagttc cataacgtga
    13261 cggcggagca gattgcgtat gttgctcagt tgcagcgttc cggcgatgaa gccggggcat
    13321 tgcaggcggc gaacgaggcc gcaacgaaag ggtttgatga ccagacccga cgcctgaaag
    13381 agaacatggg cacgctggaa acctgggcag acaggacagc acgggcattc aaatccatgt
    13441 gggatgcggt gctggatatt ggtcgtcctg ataccgctca ggagatgctg attaaggcag
    13501 aggctgcgtt taagaaagca gacgacatct ggaatctgcg caaggatgat tattttgtta
    13561 acgatgaagc gcgggcgcgt tactgggatg atcgtgaaaa ggcccgtctt gcgcttgaag
    13621 ccgcccgaaa gaaggctgag cagcagactc aacaggacaa aaatgcgcag cagcagagcg
    13681 atactgaagc gtcacagctg aaatataccg aagaggcgca gaaggcttac gaacggctgc
    13741 agacgccgct ggagaaatat accgcccgtc aggaagaact gaacaaggca ctgaaagacg
    13801 ggaaaatcct gcaggcggat tacaacacgc tgatggcggc ggcgaaaaag gattatgaag
    13861 cgacgctgaa aaagccgaaa cagtccggcg tgaaggtgtc tgcgggcgat cgtcaggaag
    13921 acagtgctca tgctgccctg ctgacgcttc aggcagaact ccggacgctg gagaagcatg
    13981 ccggagcaaa tgagaaaatc agccagcagc gccgggattt gtggaaggcg gagagtcagt
    14041 tcgcggtact ggaggaggcg gcgcaatgtc gccagctgtc tgcacaggag aaatccctgc
    14101 tggcgcataa agatgagacg ctggagtaca aacgccagct ggctgcactt ggcgacaagg
    14161 ttacgtatca ggagcgcctg aacgcgctgg cgcagcaggc ggataaattc gcacagcagc
    14221 aacgggcaaa acgggccgcc attgatgcta aaagccgggg gctgactgac cggcaggcag
    14281 aacgggaagc cacggaacag cgcctgaagg aacagtatgg cgataatccg ctggcgctga
    14341 ataacgtcat gtcagagcag aaaaagactt gggcggctga agaccagctt cgcgggaact
    14401 ggatggcagg cctgaagtcc ggctggagtg agtgggaaga gagcgccacg gacagtatgt
    14461 cgcaggtaaa aagtgcagcc acgcagacct ttgatggtat tgcacagaat atggcggcga
    14521 tgctgaccgg cagtgagcag aactggcgca gcttcacccg ttccgtgctg tccatgatga
    14581 cagaaattct gcttaagcag gcaatggtgg ggattgtcgg gagtatcggc agcgccattg
    14641 gcggggctgt tggtggcggc gcatccgcgt caggcggtac agccattcag gccgctgcgg
    14701 cgaaattcca ttttgcaacc ggaggattta cgggaaccgg cggcaaatat gagccagcgg
    14761 ggattgttca ccgtggtgag tttgtcttca cgaaggaggc aaccagccgg attggcgtgg
    14821 ggaatcttta ccggctgatg cgcggctatg ccaccggcgg ttatgtcggt acaccgggca
    14881 gcatggcaga cagccggtcg caggcgtccg ggacgtttga gcagaataac catgtggtga
    14941 ttaacaacga cggcacgaac gggcagatag gtccggctgc tctgaaggcg gtgtatgaca
    15001 tggcccgcaa gggtgcccgt gatgaaattc agacacagat gcgtgatggt ggcctgttct
    15061 ccggaggtgg acgatgaaga ccttccgctg gaaagtgaaa cccggtatgg atgtggcttc
    15121 ggtcccttct gtaagaaagg tgcgctttgg tgatggctat tctcagcgag cgcctgccgg
    15181 gctgaatgcc aacctgaaaa cgtacagcgt gacgctttct gtcccccgtg aggaggccac
    15241 ggtactggag tcgtttctgg aagagcacgg gggctggaaa tcctttctgt ggacgccgcc
    15301 ttatgagtgg cggcagataa aggtgacctg cgcaaaatgg tcgtcgcggg tcagtatgtt
    15361 gcgtgttgag ttcagcgcag agtttgaaca ggtggtgaac tgatgcagga tatccggcag
    15421 gaaacactga atgaatgcac ccgtgcggag cagtcggcca gcgtggtgct ctgggaaatc
    15481 gacctgacag aggtcggtgg agaacgttat tttttctgta atgagcagaa cgaaaaaggt
    15541 gagccggtca cctggcaggg gcgacagtat cagccgtatc ccattcaggg gagcggtttt
    15601 gaactgaatg gcaaaggcac cagtacgcgc cccacgctga cggtttctaa cctgtacggt
    15661 atggtcaccg ggatggcgga agatatgcag agtctggtcg gcggaacggt ggtccggcgt
    15721 aaggtttacg cccattttct ggatgcggtg aacttcgtca acggaaacag ttacgccgat
    15781 ccggagcagg aggtgatcag ccgctggcgc attgagcagt gcagcgaact gagcgcggtg
    15841 agtgcctcct ttgtactgtc cacgccgacg gaaacggatg gcgctgtttt tccgggacgt
    15901 atcatgctgg ccaacacctg cacctggacc tatcgcggtg acgagtgcgg ttatagcggt
    15961 ccggctgtcg cggatgaata tgaccagcca acgtccgata tcacgaagga taaatgcagc
    16021 aaatgcctga gcggttgtaa gttccgcaat aacgtcggca actttggcgg cttcctttcc
    16081 attaacaaac tttcgcagta aatcccatga cacagacaga atcagcgatt ctggcgcacg
    16141 cccggcgatg tgcgccagcg gagtcgtgcg gcttcgtggt aagcacgccg gagggggaaa
    16201 gatattttcc ctgcgtgaat atctccggtg agccggaggc gtatttccgt atgtcgccgg
    16261 aagactggct gcaggcagaa atgcagggtg agattgtggc gctggtccac agccaccccg
    16321 gtggtctgcc ctggctgagt gaggccgacc ggcggctgca ggtgcagagt gatttgccgt
    16381 ggtggctggt ctgccggggg acgattcata agttccgctg tgtgccgcat ctcaccgggc
    16441 ggcgctttga gcacggtgtg acggactgtt acacactgtt ccgggatgct tatcatctgg
    16501 cggggattga gatgccggac ttttatcgtg aggatgactg gtggcgtaac ggccagaatc
    16561 tctatctgga taatctggag gcgacggggc tgtatcaggt gccgttgtca gcggcacagc
    16621 cgggcgatgt gctgctgtgc tgttttggtt catcagtgcc gaatcacgcc gcaatttact
    16681 gcggcgacgg cgagctgctg caccatattc ctgaacaact gagcaaacga gagaggtata
    16741 ccgacaaatg gcagcgacgc acacactccc tctggcgtca ccgggaatgg cacgcatctg
    16801 cctttacggg gatttgcaac gatttggccg ccgcatcgac cttcgtgtga aaacgggggc
    16861 tgaagccatc cgggcactgg ccacacagct cccggcgttt cgtcagaaac tgagcgacgg
    16921 ctggtatcag gtacggattg ccgggcggga cgtcagcacg tccgggttaa cggcgcagtt
    16981 acatgagact ctgcctgatg gcgctgtgat tcatattgtt cccagagtcg ccggggccaa
    17041 gtcaggtggc gtattccaga ttgtcctggg ggctgccgcc attgccggat cattctttac
    17101 cgccggagcc acccttgcag catggggggc agccattggg gccggtggta tgaccggcat
    17161 cctgttttct ctcggtgcca gtatggtgct cggtggtgtg gcgcagatgc tggcaccgaa
    17221 agacagaact ccccgtacac agacaacgga taacggcaaa cagaacacct gtttctcctc
    17281 actggataac atggttgccc agggcaatgt tctgccggtt ctgtacggtg aaatgcgcgt
    17341 ggggtcacgc gtggtttctc aggagatcag cacggcagac gaaggggacg gtggtcaggt
    17401 tgtggtgatt ggtcgctgat gcaaaatgtt ttatgtgaaa ccgcctccgg gcggttttat
    17461 cgtttatgga gcatgacgaa tgggtaaagg cagcagtaag gggcataccc cgcgcgaagc
    17521 gaaggacaac ctgaagtcca cgcagctgct gagtgtgatc gatgccatca gcgaagggcc
    17581 ggttgaaggt ccggtggatg gattaaaaag cgtgctgctg aacagtacgc cggtgctgga
    17641 cagtgagggg aataccaata tatccggcgt cacggtggtg ttccgggccg gtgagcagga
    17701 gcagacaccg ccggagggat ttgaatcctc cggttccgag acggtgctcg gtacagaagt
    17761 gaaatatgac acgccgatca cccgcaccat tacgtctgca aacatcgacc gtctgcgctt
    17821 taccttcggt gtacaggcac tggtggaaac cacctcaaag ggggacagga atccatcgga
    17881 agtccgcctg ctggttcaga tacagcgtaa cggtggctgg gtgacggaaa aagacatcac
    17941 cattaagggt aaaaccactt cacagtatct ggcctcggtg gtggtggata acctgccgcc
    18001 gcgcccgttc agtatccgga tgcgcaggat gacgccggac agcaccacag accagctgca
    18061 gaacaaaacg ctctggtcgt catacaccga aattatcgat gtgaaacagt gctacccgaa
    18121 cacggcactg gtcggcgtgc aggtggactc ggagcagttc ggcagccaga aggtgagccg
    18181 taattatcat ctgcgcgggc gtgttctgca ggtgccgtcg aattataacc cgcagacgcg
    18241 gcaatacagc ggtatctggg acggaacgtt taaaccggca tacagcaaca acatggcctg
    18301 gtgtctgtgg gatatgctga cccatccgcg ctacggcatg gggaaacgtc ttggtgcggc
    18361 ggatgtggat aaatgggcgc tgtatgtcat cggccagtac tgcgaccagt cagtgccgga
    18421 cggctttggc ggcacagagc cgcgcatcac ctgtaatgcc tggctgacca cgcagcgtaa
    18481 ggcgtgggat gtgctcagtg atttctgctc ggcgatgcgc tgtatgccgg tatggaacgg
    18541 gcagacgctg acgttcgtgc aggaccgacc atcagataag gtgtggacct ataaccgcag
    18601 taatgtggtg atgccggatg atggcgcgcc gttccgctac agcttcagcg ccctgaagga
    18661 ccgccataat gccgttgagg tgaactggat tgacccggat aacggctggg agacggcgac
    18721 agagcttgtg gaggacacgc aggccattct ccgttacggt cgtaacgtca cgaagatgga
    18781 tgcctttggc tgtaccagcc gggggcaggc acaccgcgcc gggctgtggc tgattaaaac
    18841 ggagctgctg gagacgcaga ccgtggattt cagcgtcggc gcagaagggc ttcgccatgt
    18901 gccgggtgat gttattgaaa tctgcgatga tgactatgcg gggatcagca tcggcgggcg
    18961 cgtgctggcg gtgaacagcc agacccggac gctgacgctc gaccgtgaaa tcacgctgcc
    19021 atcctccggt accacgctga taagcctggt tgacggaagt ggcaatccgg tcagcgtgga
    19081 ggtccagtcc gtcaccgacg gcgtgaaggt aaaagtgagc cgtgttcctg acggcgttgc
    19141 cggatacagc gtatgggggc tgaagttgcc gacgttgcgc cagcgcctgt tccgctgcgt
    19201 gagtatccgt gagaacgatg acggcacgta tgccatcacc gccgtgcagc atgtaccgga
    19261 aaaagaggcc atcgtggata acggggcgca ctttgacggc gaccagagcg gcacggtgaa
    19321 tggtgtcacg ccgccagcag tgcagcatct gaccgccgaa gtcactgcag acagcgggga
    19381 atatcaggtg ctggcgcgat gggacacgcc gaaggtggtg aagggcgtga gcttcctgct
    19441 tcgcctgacc gtggcagcgg atgacggcag tgagcggctg gtcagcacgg cccggacggc
    19501 ggaaaccaca taccgcttca cgcagctggc gctgggacgg tacacgctga cagtccgggc
    19561 ggtaaatgcg tggggacagc agggcgatcc ggcgtcggta tcgttcagga ttgccgcacc
    19621 ggtagcaccg tcgcggattg agctgacgcc gggctatttt cagataactg ccacgccgca
    19681 tcttgcggtt tatgatccga cggtacagtt tgagttctgg ttctcggaaa cgcggattac
    19741 cgatatcagg caggttgaaa ccacagcccg ctaccttggc gcggggctgt actggatagc
    19801 cgccagtatc aatatcaaac cgggccatga ttattacttt tatatccgca gtgtgaacac
    19861 cgttggcaaa tcggcattcg tggaggccgt cggtcgggcg agcgatgatg cggaaggtta
    19921 cctggatttt ttcaaaggca agataaccga atcccatctc ggtaaagagc tgctggaaaa
    19981 agttgatctg acggaggata acgccagcag actggatgag ttttcgaaag agtggaagga
    20041 cgctaacgat aaatggaatg cgatgtgggg cgtcaaaatt gagcagacca aagacggcaa
    20101 acattatgtc gcgggtattg gcctcagcat ggaggacacg gaggaaggca agctgagcca
    20161 gtttctggtt gccgctaacc gtatcgcgtt tattgacccg gcaaacggga atgaaacgcc
    20221 gatgtttgtg gcgcagggca accagatatt catgaatgac gtgttcctga aacgcctgac
    20281 ggctcccacc attaccagcg gtggaaatcc gccggcattt tccctgacac cagacggaaa
    20341 gctgaccgct aaaaatgcgg atatcagcgg taatgtgaat gcaaattcag ggacgctcaa
    20401 caatgtcacg attaatgaaa actgtcagat taaggggaaa ctgtcagcca atcagattga
    20461 aggcgatatt gtcaaaacgg tcagcaagtc tttcccccgc acgaacagtt atgccagtgg
    20521 caccatcacg gtaagaatca gtgatgatca gaaatttgac cggcaggtca tgataccgcc
    20581 agtgttattc cgcggtggta agcatgagaa tttcaacagt aataaccaac agtcatactg
    20641 gtattcaacc tgccggttaa gagtgacccg caatggtcag gagattttta atcagtccac
    20701 gacggatgct cagggcgtat tttcctcagt tatagatatg cctgccggac aggggacgct
    20761 gacactgaca ttcaccgtat cttcatcagg agcgaataac tggacaccaa caaccagtat
    20821 cagcgatctg ctggttgtgg tgatgaaaaa atccacagca ggtatcagta tcagctgaat
    20881 tttataaccc agaacgggcg tcagaaatga cgcctttttt attgcagaaa agcgagaggt
    20941 aattatgcgt aaactttatg ccgccatttt gtccgcagcc atttgtctgg ccgtatccgg
    21001 tgcgcctgca tgggcgtctg aacatcagtc cacgctgagc gcggggtatc ttcatgcccg
    21061 gacgaacgtt cccggcagtg atgatctgaa cgggattaac gtgaaatacc gttatgagtt
    21121 tacggacaca ctggggatgg tgacgtcgtt cagctatgca ggagacaaga atcgccagat
    21181 tacccgttac agcgataccc gctggcatga agattccgtt cgtaaccgct ggttcagcgt
    21241 aatggcgggg ccgtctgtgc gcgtgaatga atggttcagc gcgtatgcga tggcgggtgt
    21301 ggcttacagc cgtgtgtcga ctttctccgg ggattatctc cgcgtaactg acaacaaggg
    21361 gaaaaagcac ga
//
LOCUS       DAJTMC010000061        20754 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.061, whole genome shotgun sequence.
ACCESSION   DAJTMC010000061 DAJTMC010000000
VERSION     DAJTMC010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20754
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.061"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(11..556)
                     /gene="hemG"
                     /locus_tag="OO130_002004"
     CDS             complement(11..556)
                     /gene="hemG"
                     /locus_tag="OO130_002004"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HCR5365431.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMSSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAQLTNKPTLK"
     gene            complement(568..2019)
                     /gene="trkH"
                     /locus_tag="OO130_002005"
     CDS             complement(568..2019)
                     /gene="trkH"
                     /locus_tag="OO130_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HCR5365432.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            complement(2058..2672)
                     /locus_tag="OO130_002006"
     CDS             complement(2058..2672)
                     /locus_tag="OO130_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HCR5365433.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGIGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            complement(2672..4003)
                     /gene="pepQ"
                     /locus_tag="OO130_002007"
     CDS             complement(2672..4003)
                     /gene="pepQ"
                     /locus_tag="OO130_002007"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HCR5365434.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHASVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHLQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFSGIRIEDNVVIHENNVENMTRDLKLA"
     gene            4193..6382
                     /gene="fadB"
                     /locus_tag="OO130_002008"
     CDS             4193..6382
                     /gene="fadB"
                     /locus_tag="OO130_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HCR5365435.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLVEGAKAVLRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDVVVEAVVENPKVKKAVLAETEQKVRPDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            6392..7555
                     /gene="fadA"
                     /locus_tag="OO130_002009"
     CDS             6392..7555
                     /gene="fadA"
                     /locus_tag="OO130_002009"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HCR5365436.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSGAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCN
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(7747..8448)
                     /gene="fre"
                     /locus_tag="OO130_002010"
     CDS             complement(7747..8448)
                     /gene="fre"
                     /locus_tag="OO130_002010"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HCR5365437.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDYQIVVDIPYGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLSELEALSLKHP
                     GLRVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(8494..9987)
                     /gene="ubiD"
                     /locus_tag="OO130_002011"
     CDS             complement(8494..9987)
                     /gene="ubiD"
                     /locus_tag="OO130_002011"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HCR5365438.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGVPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            10154..10642
                     /gene="rfaH"
                     /locus_tag="OO130_002012"
     CDS             10154..10642
                     /gene="rfaH"
                     /locus_tag="OO130_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HCR5365439.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            complement(10639..11421)
                     /gene="tatD"
                     /locus_tag="OO130_002013"
     CDS             complement(10639..11421)
                     /gene="tatD"
                     /locus_tag="OO130_002013"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HCR5365440.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDFTPKPSS
                     RRNEPAHLHHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(11463..12239)
                     /gene="tatC"
                     /locus_tag="OO130_002014"
     CDS             complement(11463..12239)
                     /gene="tatC"
                     /locus_tag="OO130_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HCR5365441.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            complement(12242..12757)
                     /gene="tatB"
                     /locus_tag="OO130_002015"
     CDS             complement(12242..12757)
                     /gene="tatB"
                     /locus_tag="OO130_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HCR5365442.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELIQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            complement(12761..13030)
                     /gene="tatA"
                     /locus_tag="OO130_002016"
     CDS             complement(12761..13030)
                     /gene="tatA"
                     /locus_tag="OO130_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HCR5365443.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            complement(13109..14749)
                     /gene="ubiB"
                     /locus_tag="OO130_002017"
     CDS             complement(13109..14749)
                     /gene="ubiB"
                     /locus_tag="OO130_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HCR5365444.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            complement(14746..15351)
                     /gene="ubiJ"
                     /locus_tag="OO130_002018"
     CDS             complement(14746..15351)
                     /gene="ubiJ"
                     /locus_tag="OO130_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HCR5365445.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            complement(15365..16120)
                     /gene="ubiE"
                     /locus_tag="OO130_002019"
     CDS             complement(15365..16120)
                     /gene="ubiE"
                     /locus_tag="OO130_002019"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HCR5365446.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            complement(16215..17642)
                     /gene="rmuC"
                     /locus_tag="OO130_002020"
     CDS             complement(16215..17642)
                     /gene="rmuC"
                     /locus_tag="OO130_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HCR5365447.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            complement(17783..18544)
                     /gene="udp"
                     /locus_tag="OO130_002021"
     CDS             complement(17783..18544)
                     /gene="udp"
                     /locus_tag="OO130_002021"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HCR5365448.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESQAVKIVVEAARRLL"
     gene            18806..19618
                     /locus_tag="OO130_002022"
     CDS             18806..19618
                     /locus_tag="OO130_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCR5365449.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGMHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKRS"
     gene            20012..20672
                     /gene="hcp"
                     /locus_tag="OO130_002023"
                     /pseudo
     CDS             20012..20672
                     /gene="hcp"
                     /locus_tag="OO130_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001680647.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
BASE COUNT         5080 a         5604 c         5210 g         4860 t
ORIGIN      
        1 ttctttatgc ttatttcagc gtcggtttgt tggttaactg ggcgatttct cgggcgaaat
       61 tcgccacctg ctcccaatcg gtatagacaa cttctttgcg cgtatccgtt tcaccgcctg
      121 acatcttcat aatcagctta atcataaaac ggtcgtacca gcgataacgt gggtaacgca
      181 gcgccccggc aatgaccgcg cagcgatcgg gacgccattg tgaactcatc agaaacttgc
      241 gcgcgtagct gttggtctgt ggagtacgct tctccggttt gcgcgccacc aggtttacgg
      301 aataaaaggc gctcggcatc gaattcagtc gcgtcgcatg ttttttgaca aattcctgga
      361 acgcagagtg gtagtgacca tagcgaatag aagcaccaat gaccacacgg tcatagtttt
      421 cccattgtgg ttcttcaatg cggtgcacat tggcgacatc cgtctggatc cccagttctt
      481 tcagttccga agccaggtag gaggcaatct cgcgcgtttg tccgtccctt gttgagaaaa
      541 gaattaatgt tttcacgtat tactccatca ttcacgccag aaagtcgggg taaagagcac
      601 cagcaatgta aagacctcga gacgaccaaa cagcatgttg gcaatcagga tccatttagc
      661 caccgggttc atactggtaa agttatcagc aaccacgcca agccctggcc ccaggttatt
      721 caatgtcgca acaactgagg caaaggcaga aaaatcatcc acgcccgtgg cgataatcgc
      781 cagcatactg acaataaaca ccaatgcata ggcggagaaa aatccccaaa cggcttcgag
      841 gatacgttcc ggcagtgcgc gattccccag cttaatgcta tagacggcgt tcggatgcac
      901 cagtcgtttc agctcacggt tcccctgctt aaacagcagc aggatgcgga tcactttcag
      961 gccaccgccc gttgacccgg cgcaaccgcc gataaaagct gaacataaaa gcagtaccgg
     1021 caaaaagagc ggccagcggg caatgctgtc agtcgtaaac ccggcggttg tcgccatcga
     1081 taccacctgg aaaaacgcct ggttaattgt catcagcgcc gaactgtaga cattatgaaa
     1141 ccacagtacg agtgtacaaa taaccaccag cgtaaactgt acgccgataa acatgcgaaa
     1201 ttccggatcg cgccaataaa ccttcagact acgcccactt aacagtgaaa agtgcagacc
     1261 gtagttacaa ccggagatca gcaggaagat agcaatgata gtgttaatag tcggactgtc
     1321 gaaataaccg atactggcat catgtgtcga gaagccgcca atagcgatag tcgcaaagct
     1381 atggccgatg gcatcaaagg catccattcc ggcaaaccac aacgccagcg cacaggcgac
     1441 ggtcagcaag acataaatca accacagggt tttcgccgtt tccgcaattc gcgggcgcat
     1501 tttgttatct ttcagcgggc cgggcatttc tgcgcgatag agctgcatcc cacccacgcc
     1561 gaggataggc agtatcgcaa ccgctaacac gatgatcccc atcccgccaa accattgcag
     1621 catctggcga taaaagagga tggcatgagg gagcgaatcc agccccacca gcgtagtggc
     1681 cccggtggtg gtcaggccag agaaagattc aaaaaacgca tcggtaatcg tgaggttcgg
     1741 gctttccgag aagataaaag ggagcgcacc gacactaccc agcacggtcc aaaacagcac
     1801 cactatcaga aacccctcac gggatttcag ttcgcccttc tctttgcggt tcggccacca
     1861 cagcatagag ccaatggcga gggcgacaaa aaaggtctgg gtaaaagcgc ggcccgctcc
     1921 atcccggtag atgagtgcta ccagcccagg gataatcatg gtccctgaaa ataagatgac
     1981 cagtagtcca acgattcggg taatggcgcg aaaatgcatc tctgccgctt ccttagttct
     2041 gcaaaatgaa gtggggatta ttcttcaatc gctaacaatt gcaatgaacc acggctaaaa
     2101 tccgccagct ttgcggaaaa ttcagccact ttcgccgccg gaagcgccac ccgcagcaga
     2161 acgaatgcct gataatcact gttgataatt ttgccgtcac actgccccag caacgcttca
     2221 atgccggtta actgactata ttcacattgc aaagtatatt cggttaatgg cgtcttgcgt
     2281 tgggtcgtta gctggcgcag cgcctgattc acgccgccgc catacgcttt cactaaccca
     2341 ccggtgccaa gcaatatgcc gccgtagtag cgcactacca cagcggtaat ttccccgatg
     2401 ccgctgccca ttagctgggc gagcatcggt ttacctgccg ttcccgccgg ctccccgtcg
     2461 tcagagaaac ccagctgttg agaatcatcc ggcgcacccg ccacccacgc cacgcaatgg
     2521 tggcgggcat cggggtgttc tgcccgcacc gattcaacaa acgctttcgc cgcctcaacg
     2581 ccatcggtat gcgccaacat cgttatgaag cggctctttt tgatctcttc aacgaccgtg
     2641 accggtgccg caggaattaa ccagctttcc atcacgccag tttcagatcc cgggtcatgt
     2701 tttccacgtt attttcgtgg atcaccacgt tgtcttcgat acgaatgccg ctgaacggtt
     2761 tcagtgcttc aattttctgc cagttgaagt gcttgctgaa ctgcccttca cgccacggtg
     2821 ccagtagcga ttcgatgaag tagatacccg gttcgatggt taacaccatg cccggctgga
     2881 gaatacgggt gcagcgcagg tacggatatt ttgccggtgc cgcgaggtgt gtaccgctat
     2941 catcctgcat aaaaccggct acgtcatgca cctgcaggcc cagcggatgg ccgataccgt
     3001 gcggcataaa cggtccggtg agatcgtttt cgaccatcgc ttcttcactc atatcggtga
     3061 tgatttgatg tttacgcagc aatttggcga tgcgctgatg gaactggagg tggtaatcca
     3121 catagctgac gccagctttc atggtagcga tcagcgcaag ttgttcatca tttacgtctt
     3181 tcaccagctg tgcgtagtcg ttgtcgcttt ttgccgacca ggtacgagtc aggtcagccg
     3241 catagccgtt atattcggcc ccggcatcca gcaggaagct gcgcatctct tccggtgcct
     3301 gatgatccag tttggtgtaa tgcagcaccg aagcgtgttc gttaagcgcc acaatgttgc
     3361 tgtaaggtac gtcggtatca cgatgaccgg tcgcggtcag ataggcgatg ttgatatcga
     3421 actcgctcat gccagaacgg aacgcttctt ctgccgcacg atgaccgttg accgccattt
     3481 tctgcgcttc acgcatacag gccagttcgt actcggtttt gaaggagcgg tagtaatgca
     3541 ggtagtcgat cacccctttc gggttgatgt tgctggcctc aatacccagt tgcagcgcac
     3601 gttccggcac cggaccgata taaccgatat tgccgcgcgc agcgggcagc aggctaccaa
     3661 tgccatcggc tttcggcagt gcgatcactt ccacatcttc agtccagaag gaggtcggca
     3721 gcggttcgac gttgtgccag taatcaaccg gcagatagaa ccacagtttc ggcttgttca
     3781 cgccatccac cagcaaccag cagtttggca cctgagttac cggcacccac gctttgaatt
     3841 gcgggttcac tttaaacgga tagggatgat cgtcgagaaa aacgttgaac agctcgccgg
     3901 agtgaataag taatgcatcc agcttgaagc gcgccagcgc atcgcgagtc cgttcttgta
     3961 aggtagctat atgattttta tagagcgagg ccagtgattc cattttttac ccttctgttt
     4021 ttttgacctt aagtctccgc atcttagcac atcgttcatc cagagcgtga tttctgccga
     4081 gcgtgatcag atcggcattt ctttaatctt ttatttacat gtttttaaca caaaatacac
     4141 acttcgatgc atctggtacg accagatcac cttgcggatt caggagactg acatgcttta
     4201 caaaggcgac accctgtacc ttgactggct ggaagatggc attgccgaac tggtatttga
     4261 tgccccaggt tcagttaata aactcgacac tgcgaccgtc gccagcctcg gcgaggccat
     4321 cggcgtgctg gaacagcaat cagatctaaa agggctgctg ctgcgttcga acaaagcagc
     4381 ctttatcgtc ggtgctgata tcaccgaatt tttgtccctg ttcctcgttc ctgaagaaca
     4441 gttaagtcag tggctgcact ttgccaatag cgtgtttaat cgcctggaag atctgccggt
     4501 gccgaccatt gctgccgtca acggctatgc gctgggtggt ggctgcgaat gcgtgctggc
     4561 gaccgattat cgtctggcga cgccggatct gcgcatcggt ctgccggaaa ccaaactggg
     4621 catcatgccg ggctttggcg gttctgtacg tatgccacgt atgctgggcg ctgacagtgc
     4681 gctggaaatc attgccgccg gtaaagatgt cggcgcggat caggcgctga aaatcggtct
     4741 ggtggatggc gtagtcaaag cagaaaaact ggttgaaggc gcaaaggcgg ttttacgcca
     4801 ggccattaac ggcgacctcg actggaaagc aaaacgtcag ccgaagctgg aaccactaaa
     4861 actgagcaag attgaagcca ccatgagctt cactatcgct aaagggatgg tcgcacaaac
     4921 ggcggggaaa cattatccgg cgcccatcac cgcagtaaaa accattgaag ctgcggcccg
     4981 ttttggtcgt gaagaagcat taaatctgga aaacaaaagc tttgtcccgc tggcgcacac
     5041 caacgaagcc cgcgcactgg tcggcatttt ccttaacgat caatatgtaa aaggcaaagc
     5101 gaagaaactc accaaagacg ttgaaacacc gaaacaggcc gctgtactgg gcgcaggcat
     5161 catgggtggc ggcattgctt accagtctgc gtggaaaggc gtaccggttg tcatgaaaga
     5221 tatcaacgac aagtcgttaa ccctcggcat gaccgaagcc gcgaaactgc tgaacaagca
     5281 gcttgaacgc ggcaagatcg atggtctgaa actggctggc gtgatctcca cgatccaccc
     5341 aacgctcgac tacgccgggt ttgaccgcgt ggatgttgtg gtagaagcgg ttgtcgaaaa
     5401 cccgaaagtg aaaaaagccg tactggcaga aaccgaacaa aaagtacgcc cggataccgt
     5461 actggcgtct aacacttcaa ccattcctat cagcgaactg gccaacgcgc tggaacgccc
     5521 ggaaaacttc tgcggaatgc acttttttaa cccggtacac cggatgccgt tggtagaaat
     5581 tattcgcggc gagaaaagct ccgacgaaac catcgcgaaa gttgtcgcct gggcgagcaa
     5641 gatgggcaag acgccgattg tggttaacga ctgccccggc ttctttgtta accgcgtgct
     5701 gttcccgtat ttcgccggtt tcagccagct gctgcgcgac ggcgcggatt tccgcaagat
     5761 cgacaaagtg atggaaaaac agtttggctg gcctatgggt ccggcatatc tgctggatgt
     5821 cgtgggcatt gataccgcgc atcacgctca ggctgtcatg gcagcaggct tcccgcagcg
     5881 gatgcagaaa gattaccgcg atgccatcga cgcgctgttt gatgccaacc gctttggtca
     5941 gaagaacggc ctcggtttct ggcgttataa agaagacagc aaaggtaagc cgaagaaaga
     6001 agaagacgcc gccgttgaag acctgctggc agaagtgagc cagccgaagc gcgatttcag
     6061 cgaagaagag attatcgccc gcatgatgat cccgatggtc aacgaagtgg tccgctgtct
     6121 ggaagagggc attatcgcca ctccggcgga agcggatatg gcgctggtct acggcctggg
     6181 cttccctccg ttccacggcg gcgcgttccg ctggctggac accctcggta gcgcaaaata
     6241 cctcgatatg gcacagcaat atcagcacct cggcccgctg tatgaagtgc cggaaggtct
     6301 gcgtaataaa gcgcgtcata acgaaccgta ctatcctccg gttgagccag cccgtccggt
     6361 tggcgacctg aaaacggctt aaggagtcac aatggaacag gttgtcattg tcgatgcaat
     6421 tcgcaccccg atgggccgtt cgaagggcgg tgcttttcgt aacgtgcgtg cagaagatct
     6481 ctccgctcat ttaatgcgta gcctgctggc gcgtaacccg gcgctggaag cggcggccct
     6541 cgacgatatt tactggggtt gtgtgcagca gacgctggag cagggtttta atatcgcccg
     6601 taacgcggcg ctgctagcag aagtgccgca ctctgtcccg gcggttaccg tcaatcgctt
     6661 gtgtggttca tccatgcagg cactgcatga cgcagcacga atgatcatga ctggcgatgc
     6721 gcaggcatgt ctggttggcg gcgtggagca tatgggccat gtgccaatga gtcacggcgt
     6781 cgattttcac cccggcctga gccgcaatgt cgccaaagcg gcgggcatga tgggcttaac
     6841 ggcagaaatg ctggcgcgta tgcacggtat cagccgtgaa atgcaggatg cctttgccgc
     6901 gcggtcacac gcccgcgcct gggccgccac gcagtcgggc gcatttaaaa atgaaatcat
     6961 cccgaccggt ggtcacgatg ccgacggcgt cctgaagcag tttaattacg acgaggtgat
     7021 tcgaccggaa accaccgtgg aagccctcgc cacgctgcgt ccggcgtttg atccagtaaa
     7081 cggtacggta acggcgggca catcttctgc actttccgat ggcgcagctg ccatgctggt
     7141 gatgagtgaa agccgcgccc atgaattagg tcttaagccg cgcgctcgtg tgcgttcgat
     7201 ggcggtcgtt ggttgtaacc catcgattat gggttacggc ccggttccgg cctcgaagct
     7261 ggcgctgaaa aaagcggggc tttctgccag cgatatcggc gtgtttgaga tgaacgaagc
     7321 ctttgccgcg cagatcctgc cgtgcattaa agatctcggg ctaatggagc agattgacga
     7381 gaagatcaac ctcaatggtg gtgcgatcgc gctgggtcat ccgctgggct gttccggtgc
     7441 gcgtatcagc accacgctgc tgaatctgat ggaacgcaaa gacgttcagt ttggtctggc
     7501 gacgatgtgt atcggtctgg gtcagggtat tgcgacggtg tttgagcggg tttaaccact
     7561 tgccggatgc ggcgtgaacg ccttatccgg tctaccgatc ctgcaccaat gtaggcctga
     7621 taagacgcgc cagcgtcgca tcaggcatcg tgcaccgatt gccggatgcg acatagatct
     7681 aaatgccact gaataaagtt ttagttgccg ttcttcccgc ctgtcagggg cgggtttttt
     7741 tatatctcag ataaatgcaa acgcatcgcc aaacagacga tcttcccgcg cattacgctc
     7801 actgcaaaac aggtcacgag caattttcgc catctcaaaa cgtccggcaa tatagatatc
     7861 atgctctgcc agcgtaccat gatcctgcaa gaccgccgtt aacacggtgc cagtacgccc
     7921 acgccagccc gcttccggtt gttcaaccac cggcaccaca cgcaggccag gatgcttcag
     7981 cgaaagcgcc tcaagctcgg agagatcata aagatgttgc tcctcacgcc caccccagta
     8041 aatggtgata tcgcgattgg gattacgcgc caacgccgtc aataaaatcg agcgcgcata
     8101 ggagaacccg gtgccgcccg caatcaaaat catcggacgc tcttcatcat cgcgcagcca
     8161 cgcttctccg tagggaatat cgaccacgat ttgataatct ttgaggatgc ggtccatgac
     8221 tgctttcgcg taaaggttga tttcagaagc gccaatatgc agctctataa accctttttc
     8281 atccggcgtc gaagccattg aaaacggacg tttgtcgcgc tcatccatca ctaccatcaa
     8341 atactgacca gcacgaaaag aaaaggccgc gtctggcacg atgcggacac gatatacggt
     8401 atccgtgata gcttctaccg aggtcacttt acagcttaag gttgtcatgc gctttctctg
     8461 tcggatcgat aaatagggca aaacaaacgc gcatcaggcg cttttaccgt tgttaaaaat
     8521 agccagttca tcccagatgg cgtcaatatg cgcgacaaca tctggatctt ttttgatggg
     8581 acgtccccat tcacgctggg tttcccccgg ccatttattc gtggcatcca gccccatttt
     8641 tgaacccagc ccggagacag gcgaggcaaa atccagataa tcaataggcg tattttctac
     8701 cagaacagta tcccgcgccg ggtccatacg ggtggtaatc gcccaaatca catcgttcca
     8761 gtcgcgtgcg ttgacgtcat catcgcaaac gatcacaaat ttagtgtaca taaactggcg
     8821 taagaacgac cagacgccca tcatgacgcg cttcgcgtgt ccggcgtact gttttttgat
     8881 cgtcactacc gccagacgat aagaacagcc ttccggcggc aggtaaaaat cgacaatttc
     8941 cgggaactgt ttttgcagaa tcggtacaaa cacttcgttc aacgccacgc ccagtaccgc
     9001 gggctcatct ggcggacgcc cggtataggt ggaatggtaa atcgcatctt cacgctgggt
     9061 aatatgcgtc acggtaaata ccgggaaact atcgacttcg ttatagtaac cggtgtggtc
     9121 gccatacggc ccttccggcg cagtttcgcc ttgttcgata tacccttcca gcacaatctc
     9181 cgcactggcg ggcacttcaa ggtcattgga gatacacttc accacttcgg tcttggtacc
     9241 gcgcagcaat ccggcaaacg catactctga aagtgtatcc ggaacggggg tgactgcacc
     9301 gagaatcgtg gcgggatcgg cacccagcgc cacagaaacc gggaaacgtt cgcccggatg
     9361 cgccgcacac cactcctgat aatccagcgc gccgccgcga tgcgacagcc agcgcataat
     9421 cagtttgttt ttaccaatca gctgctggcg ataaatgccc agattctgcc gctctttatg
     9481 cgggccgcgc gtcactgtca gcccccaggt aatcagcggt gcggcatctt ccggccagca
     9541 ggtcataatg ggaatgcgat tgagatcgac gtcatcgcca gagacgattt tttgttggca
     9601 gggcacacca cgcagccgct ttgtcggcat gttcaatact tgcttaaact gcggcagttt
     9661 atcaaacagg tcgcggaaac cttttggcgg ctccggctct ttcagaaacg ccaataattt
     9721 accaacttca cgcagcgccg aaacatcttc ctgtcccatg cccatcgcca cgcgctttgg
     9781 cgtaccgaac aggttgcaca gcaccggcat tgagtagcct ttagggtttt cgaacaacag
     9841 cgcaggccca ccggcacgca aagtgcggtc agcaatttca gtgatttcca gatgcggatc
     9901 caccgggagc gtgatacgtt ttagctcacc ctgctgttca agcagcgtca agaagtcgcg
     9961 taaatcgtta tatttcatgg cgtccattgt agcctcttaa tctacgccca ttatacggcg
    10021 ttcatctttg cgatgctgta aatttgttaa attagcgtga actctgacgg tataacgcaa
    10081 accggggaat ataattaact tagcgtaaag cttttgctat ccttgcgccc cgattaaacg
    10141 gataagagtc attatgcaat cctggtattt actgtactgc aagcgcgggc aacttcaacg
    10201 tgcccaggaa cacctcgaaa gacaggctgt gaattgcctg gcaccgatga tcaccctgga
    10261 aaaaatcgtg cgtggaaaac gtactgcagt cagtgagcca ttgtttccca actacctgtt
    10321 tgtcgaattt gatccagaag tgattcatac cacgactatc aacgcgaccc gcggtgtcag
    10381 ccacttcgtg cgctttggcg cgtcgccagc gatagtccca tcggcggtga ttcatcagct
    10441 atcggtatat aaaccgaaag acattgtcga tccggcaaca ccttatccgg gtgataaggt
    10501 gattattacc gaaggcgcgt tcgaaggctt tcaggccatt ttcaccgaac ccgatggtga
    10561 ggctcgttcc atgctattgc ttaatcttat taataaagag attaagcaca gtgtgaagaa
    10621 caccgagttc cgcaaactct aaaacgcaat cccaaacagt gttttgacat tggcatccgt
    10681 ggtggcagcc agccatgcgg catcttctcc acgccagtgc gcaatacgtt gcaaaatatg
    10741 gtgcagatgg gctggctcgt tgcgccggga tgatggcttt ggcgtgaaat cgcgcgggag
    10801 cagatacggc gcatcagttt cgatcagcaa tttctccgcc ggaatcaacg gcaacaattc
    10861 ccgcagctcc agcccgcgtc gttcatcgca aacccaaccg gtaatgccga tataaattcc
    10921 acgcgccacg cacgcctgca tctcttcgcg tgtgccggta aagcaatgaa gaaccgcacc
    10981 aggcagttta tccagccacg gctccagcaa tgtcataaac cgctcgtggg catcgcgaca
    11041 gtgcataaat accggcatgt ttaattctgc ggcaatgcgt agctgagcaa caaaagcgcg
    11101 ttcctgctct tccggcgtcg aaaagttgcg gttaaagtcg agaccacatt caccaatcgc
    11161 caccacttct ggctgcgcgg ccagctcaat aatcgcttct tcagtcgcag cttgccactg
    11221 gctgctgtcg tgaggatgta cgcccgccgt tgaccaacag gacgaatact gacgcgccag
    11281 cttttgcgcc tgctggcttt cacgcaggtt agtgccggtg atgagtagcc cattaactcc
    11341 cgcgtcaaaa gcgcgcgcta caacatcatc acggtctttc gcaaattgcg aactggtcaa
    11401 attaacgccg atatcaaaca tcctgtactc catatgacaa ccgccctgac gggcggttga
    11461 atttattctt cagttttttc gctttctgct tcagcgtcgt tttcctcttc ccgatttcgc
    11521 cctttaccaa cgtaaaagcg tgagaagaag acaccgattt caaacagaca gtacatcggg
    11581 atcgccaata gcgtttgcga gaagacatcc ggcggcgtca gcaacatccc gacaacgaat
    11641 gcaccaacca gcacatacgg gcgttttttg cgtaagtctt ctggcgaggt aatccccatc
    11701 cagcacagca gcacaattgc caccggcact tcaaaggaga caccaaacgc cataaacagc
    11761 gccataacga agcttaaata gctggcgatg tcggtggata cctgcacccc ttccggcgcg
    11821 gtattggcaa ggaagccaaa tgccagcgga aagaccacaa agtaggcgaa tgccatgccg
    11881 atataaaaca gcagagagct ggaaaccagc agcggcacca ccaggcgacg ttcatgctta
    11941 tacagcgctg gggcgataaa tgcccacacc tgatagagaa tcaccggcgc tgacagaatc
    12001 agcgacacca taaaggtcag cttgatcggc gtaaagaacg gcgaggccac gtcggtggcg
    12061 atcatcgttg aaccttgcgg caactgcttg atcaatggcg cggataccag gtgatagatg
    12121 tcattggcga aatagaccag acacaggaat atcacgatca ccgcgataat gcagttcagc
    12181 agacgcttac gcagctcaat tagatgcgtg ataagcggtt gagtatcttc tacagacatg
    12241 tttacggttt atcactcgac gaaggggaag gtgcagcggt tttcggttca gcgtccgcag
    12301 caggttttac caccggctct ggcgtggttt ctggcttctg ttccggcgaa ctggcctgcg
    12361 tttgtgcagc ggcaggcgtt acgccctcat gcgcagcttc attatctttc accaccgggt
    12421 tatggatggt gtgcgcttca tcgctcgcct tttctggatc gtttgcaacg taggaacgct
    12481 tcatcgactc cgcggcctgg cgtagttcat ccatcgacgc tttcagttcg ggcgtcaggt
    12541 tagtgaggct cgccttttca acctttttca gactgtcctg aaactcctgg agttttaact
    12601 cctggatcag ttcgttctgc accgttgtcg ccagtgaacg caacgcgcga atccagcccg
    12661 ctaccgtttt taccgccaca ggcagtcgtt gcggccccag aacgacgagg ccgatgatga
    12721 acaccaatag cagttcgcta aaaccgatat caaacacgga ttacacctgc tctttatcgt
    12781 ggcgcttcgc gtcttctgtt ttagcctgtt cctgattcgt atccgcctgc ttatcggcga
    12841 tagttttcgc agtaaaatca gcatcctgac tggttttatc ctgctttggt tcatcatcgc
    12901 tcattgcttt tttaaagcct ttgatcgacg caccaagatc ggaaccgatg gagccgagct
    12961 ttttggtgcc aaaaagcagt acaacgatga cggcaataat caataactgc caaatactga
    13021 taccacccat acatgttcct ctgtggtaga tgatgattaa acaaagccgc attatacgtt
    13081 acacggcccg ccttgagcga tgaaaaaatc agcgtgtttt gcgccaaccg acaaaccagg
    13141 cgatcagacc acctgccatt aaccagccgg gcatcagccc ccactcaggt cggctgacca
    13201 acaagaatgt gccacttaat actaacgtag cgccaattcc gagaaaataa cgcgattgtc
    13261 cctgacgtac atgatttgac tgaagctcgc gggcaatctt atcaacactg tgctgtaaat
    13321 acttgccctg gcgcaaactg tcgtaaacca attcaggcag ttctggcatt ttttcgaccc
    13381 agaacggcgc tttttcttta aatgctctca ccagcgcagg aataccgacc tgatctttaa
    13441 tccacgactc caggaaaggc ttcgctgttt tccataaatc gagctgcgga taaagctggc
    13501 gtcctacccc ttcgacatag agcagggttt tctggagtaa caccagttgc ggctgcactt
    13561 ccatattgaa gcgacgcgcc gtattaaaca gatttaacag tacatgtcca aacgaaattt
    13621 cggccagcgg tttctcaaag ataggttcac agaccgtacg aatggcaaat tcgaactctt
    13681 caacgttggt atctggtggc acccagcctg aatcgacgtg tagctctgcc actttgcgat
    13741 agtcgcgatt aaagaaggcg ataaagtttt ctgccagata gcgtttatct tctttgttta
    13801 gcgagccaac aatcccgcaa tcaatgccga tatatttcgg gttttccggg tgttcatagc
    13861 ttacgaagat gttgccaggg tgcatatcgg catggaaaaa gctgtcgcga aagacctgag
    13921 tgaagaacac ctgcacgccg cgttccgcca gcaatttcat gttagtgccg tttttctcca
    13981 gcgccgcaac atcagacacc ggaatgccgt aaatgcgctc catcaccatc atcccttcac
    14041 tacaatagtc agggtaaact tccgggatgt agagcatcgg gctgtcttca aaattgcgcc
    14101 gaagctgaat ggcgttggca gattcccgca gcaaattcag ttcatcaatc aaggtctttt
    14161 cgtactcgcg caccacttcg gttgggcgca gacggcgacc atccggcagc aaacgcggca
    14221 cccagcgagc cagacggtag ataagtttca gatccgcttt aatgaccggc aaaatatccg
    14281 ggcggatgac tttaatcacc acctctttac cattcgattt caatcgcgcg gtatgaacct
    14341 gggcgataga agcagaagcc agcggcttga tttcaaaatc gtcaaaccac gcttctaccg
    14401 gcaagccgcc cattgcagct tcaatctgct gcttcgccag cttgccatca aacggagcta
    14461 ctttgtcctg caataacgcc agctgatcgg caatatgcgg tggaaaaaga tcgcggcggg
    14521 ttgataacat ttgcccgaac ttgatccaaa ccggccccag ttcttgcagg gccagtcgta
    14581 gtcgctcacc taaaagtttg tctttatgcc gatttggcat ccagaataat gagtatcgcc
    14641 atagccgtag cggcagggtg atacgcattt tggggatcag ttcatcaagt ccgtagctta
    14701 aaaaagtgcg aatgatgaaa tataggcgcc gtacttcacc tggcgtcatt tagcctccag
    14761 tttttccagc cgtttggtca gggcatcaac agcacgctcg acggcagccg tctcttccgc
    14821 aaaccaggcc acttcaagcg gaccgggtgc catacgccac tcttcagtaa tggcttccgc
    14881 cacataacgt tgctggcgct taatgccgtg atgcaggaac tttgcgcctc cgcgcatggc
    14941 tttgctgatt ccttcagcgg cgatatcacc ggtataaggg gccagcagtt ccgcagggtc
    15001 gaactctgcc agatctgcca gcgcaacgaa gttttgcacc acctgaatat cgccctgcac
    15061 ttccagctca ccactgcgaa tcagtgcggt aagctgctgg cgatcgcgaa gtttcggcaa
    15121 cacactggcg taggcgataa cggtgcagtc agcatcgcct gcccattcgc ccagtacatc
    15181 aacctggcgt tcgctgaaca ccagaatcaa tgacgtcgaa aagcctttta cctccacgcg
    15241 caatacttta cccagcagac gcgagcgggc tgttttcagc gcgggtgagc gatacaggaa
    15301 ggtgttgagc agactttcaa ttcctgccgt cactaaaggt ttaaaaggca tttccggtct
    15361 cctgtcagaa cttataacca cgatgcagcg ccacaacccc tgccgtcaga ttgtagtagt
    15421 cgacactttc gaatccggca tcctgcatca tggctttcag ggtatcctga tcgggatgca
    15481 tacggatgga ttctgccaga taacggtagc tgtcggcgtc gttcgcgacc agtgagccaa
    15541 tacgcggcag cacatggaag gagtatgcat cataggcttt gctcagcggc tcgataattg
    15601 gcttcgagaa ctcaagcacc agcaggcggc cgccgggttt cagcacgcga tacattgaac
    15661 gcagtgcttt atctttgtcg gtgacgttac gcagaccaaa cgaaatggtg atgcaatcaa
    15721 aggtgttatc cgggaacggc agcgcctcag cgttcgcctg aacatactca acgttgccaa
    15781 tcacaccgat attacgcagc ttctcgcgtc ccattttgag catggattca ttgatatcag
    15841 caaggaccac tttgccagtt tctccgacca ggcgggagaa tttcgctgtc aggtcgccgg
    15901 tgccaccagc cagatccagc acggtctgcc cacggcgtac gccgctgcaa tcaatcgtga
    15961 atcgcttcca caaacgatga ataccaaatg acatcaaatc attcatgaca tcgtattttg
    16021 atgccacgga atggaaaacg tgggcgacca tatccgcttt ttgttccttc gcgacggtct
    16081 gaaaaccaaa gtgcgtcgtt tcttgtgact tatccaccat ctcaatgcct gctcatcaaa
    16141 aaattgttcc agaagtgtaa cagattgggc gtcgatgccc tagatttcta cccggcttaa
    16201 ctacccctaa tgggctagcg cgactgctga ttatattcat catcgcgttg ataagcttca
    16261 tcattcggct gctccggaac cgaccgaagt cgatactctt catcctggct caccgcctgt
    16321 tcagccaaat ccggattaat ctcgcgttta atttctactc ctaaaccgcg aaacgcttct
    16381 gcctgcgcca gcacatttcc gcgacctgaa gagagttttt tcattgcctg gcggtagtta
    16441 tcctgcgctt tgtcgagact ttgaccaatc gcggacatgt catcgatgaa caaacgcatc
    16501 ttgtcgtaca gcttgctggc acgatcggcg atttgctggg cgttgcggct ttgatgctca
    16561 taacgccaca ggttggcaat agtacgcaac gccaccagca gcgtagtcgg gctaaccagc
    16621 atgatattgt ttttcaacgc ttcggtgatc agctccggct ggcggtcaag cgccagtaaa
    16681 aaagcgggtt caacgggaat aaacatcagc acgtaatcca gagttcgcag ccccggcagc
    16741 tgttgataat ctttgcgtcc cagcaaacgg atatggttac gcaccgacgc gatatgttcc
    16801 tgtagcgcgc tttcgcgggt gtagtcgtct tcggcgttaa aatagcgttc ataggcgacc
    16861 agcgtcattt tggcgtcgat caccacatct tttccctgcg gcagccgcac gatgacatcc
    16921 ggctgcatcc gcgagcgggc gtcattttcg atgctgacct gggtttcata ttcataccct
    16981 tcacgcagac cggaagcctc cagcacccgc gtcaatacta cctcgcccca gttgccctgg
    17041 gttttattgt cgcctttcag cgcgcgcgtc aggttgatcg cttcctgggc catttgcgcg
    17101 ttgagttgct ggagattgcg aatttcgtgg gtcagggtat ggcgttcttg tgcttcttta
    17161 ccgaagctgt cctgaacctg acggcggaaa ccgtccagtt gttcacgtag cggcgacaac
    17221 aggctgttca gactctgacg gttttgctca tcaacccggc gattgctgtg ctcaaaaata
    17281 cggttggcga ggttttcaaa ctgctcactg aggcgctgct cgctgttaat catctggcga
    17341 attttatcgt cagcatgttg ctgtgcggct tccatccgcg tggttacttc acgcagatcg
    17401 gcctccagag aggtgttaat actttgcagg ctgcgcactt cgttattgag taactcgcac
    17461 tctgcacgcc agtgctcgct ttgggtaatt tgttgttttg ccgcgcttaa ctccgcgacc
    17521 atctcttcac gttcagctaa ttgctcggct ttttgctgcg catgttgata actggcaaac
    17581 agccagccaa ttgccacacc caccaacgca ataactgcgt aaaccatgat tgagaaatcc
    17641 acaacgcctc ctgacgcaac aacttacccg cacaaaatag aattgtgctg tacaaacgtc
    17701 cagttggaaa ggcatttcct gcgaggcgct acgcaaaaat acaaaaggcc gaacgcgtcg
    17761 gccttcagac aggagaagag aattacagca gacgacgcgc cgcttccacc acgattttca
    17821 ccgcttggct ttcagtttgt ttcatcgtct cagcattcgg gatctcttgc tgggtgcggt
    17881 taacgataac acccgctacc ataccggcac gcaggccctg acttgcacac atggtcagca
    17941 gggttgcaga ttccatttca tagttcatta cgcccatcgc ctgccactct tccatagaac
    18001 ctttaaagtg acgaactacg cgaccagagt aagtatcgta acgttcctgg cctgggtaga
    18061 aggtatcaga agaagctgtc acgccaacgt gagttgtcgc gccaatggat ttcgcagctt
    18121 caaccagcgc agtcgtacat tcgaaatcag cgacagccgg gaattccagc ggtgcgaagt
    18181 gcaggctcgc gccatccaga cggacagacg ccgtggtaac caggacatca cccacattaa
    18241 tatgcggctg aatagcgccc gttgtaccga tacgcaggaa ggtgcgaatg cccagctgtg
    18301 ccagctcttc aacagcaata gaggtagacg ggccgccgat accggtagag cagacgataa
    18361 caggtttacc atccagctct gcacgccagg tggtgaattc gcggtgagat gccagcttaa
    18421 ccggcttatc catcagcgcg gcgatctttt ccacacgatc cgggtcgcca gggacgatgg
    18481 caagcgtagc cccttgtaaa tcgtttttag tgaggccgag atgaaaaaca tcagacttgg
    18541 acatatacaa ctcctctgtg aatcggttta gtcagaagaa gcaaaaagac actttaccga
    18601 agggtttaac attttttcgt gatactcatc accatgacgc aaatgcgttg cataaattta
    18661 cccaccaaaa gtgatgcaaa tcacataaat cactcttcat tctgcaggac tgcataaaag
    18721 atgagccgat tcaggcaatg tgatttgttg catgctgact atagttaaca ttgcgcaaca
    18781 tttttataag ggtacggaga ataccatggc aacaacacaa caatctggat ttgcacctgc
    18841 tgcatcgcct ctcgcttcga ccatcgttca gaccccggac gacgcgattg tggcgggctt
    18901 cacctctatc ccttcacaag gggataacat gcctgcttac catgccagac caaagcaaag
    18961 cgatggccca ctgccagtgg tcattgtagt gcaggaaatt tttggcatgc atgaacatat
    19021 ccgcgacatt tgtcgccgtc tggcgctgga ggggtatctg gctatcgcac ctgaacttta
    19081 cttccgcgaa ggcgatccga atgattttgc cgatatcccc acgttgctta gcggtctggt
    19141 agcaaaagtg cctgactcgc aggtgctggc cgatctcgat catgtcgcca gttgggcttc
    19201 ccgcaacggc ggcgatgttc atcgtttaat gatcaccgga ttctgctggg gtggacgtat
    19261 cacctggctg tatgccgcgc ataatccaca gctaaaagcc gcagtggcgt ggtacggcaa
    19321 actgacgggc gacaagtcgc taaattcacc gaaacaacct gttgatatcg caaccgatct
    19381 taacgcgccg gttctcggct tatatggtgg tcaggataac agcattccgc aggagagcgt
    19441 tgaaaccatg cgccaggcgc tgcgggctgc taatgcgaaa gcagagatta tcgtgtaccc
    19501 ggacgccggg catgcattca acgctgatta tcgcccgagc tatcatgccg aatctgcgaa
    19561 agatggctgg cagcgtatgc tggaatggtt taagcagtat ggtgggaagc ggagttagtt
    19621 tcctcttagt ctatatttga gatggtgagt gaacgagtgc gttttgctgc catctctgtc
    19681 ccccaaaaaa taatcagtat gaattgattg gagattacga ctccgctatt tgaccttaag
    19741 aaagtcggca agtcatagtc ataaatgagt ctcttcaatg gaagacacct tagagattta
    19801 ccatctactc ccattcacaa acaaacatta attattatca acacattcac attaattata
    19861 aatcatctca gaccttcctg ataaaaagat attaaagtga aacattttgt ttttcacatt
    19921 ttagttcatc acttttaggc gtaaataaag gtaaaaaaaa caacaatact atttctcttt
    19981 cacaaaagaa ttaacaaaag gttttttaag gatgagtgat attatttact tgaccataac
    20041 cggagaacaa caaggatgta tttcctctct ttgtggaacg agcgcatcaa taggtaatcg
    20101 ctggcagatt ggccatgaag atgaaatctt cgcgttctcg ctctctaata gtattacgaa
    20161 tgcaggcaaa gggtcacaat tgcatggcct tagtttctgt aaattcttat taacgctatt
    20221 aacaataacg agcagttgtt catggcgttt gacttctatc ggatcaatag atttggtcga
    20281 tgggagaaat attacaacat tcaactccgc ggcgcgctct tatcagcaat caatcacctg
    20341 tttactgaga acaatctgga tacagaagcc atcactgtca gttatgaata tatactctcc
    20401 aggcatctga ttgctaatac tgaatttagt tatttggcat ttcccgataa ctacaaccgt
    20461 ctgtttatac cacggccaaa aactaaagct ggtaatggtt caaaacactt aatagtaaag
    20521 cggttggcag actgcttgct gctggcggca tatacaatgg gaatatcgaa ggattcaggg
    20581 aaacagcaga caagttgggg ggagatgcac ctgctggtta cgatcaattt atggataaca
    20641 aagggttact catcgctggt gcttcaatag ttgctggagc agttctgggg agaatgagac
    20701 tccctgattt ggaatcactt gagcatttcg gagccagagg agcagtatca ggaa
//
LOCUS       DAJTMC010000062        20720 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.062, whole genome shotgun sequence.
ACCESSION   DAJTMC010000062 DAJTMC010000000
VERSION     DAJTMC010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20720
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.062"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(73..4533)
                     /gene="mukB"
                     /locus_tag="OO130_002024"
     CDS             complement(73..4533)
                     /gene="mukB"
                     /locus_tag="OO130_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HCR5365450.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLDGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVVFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVCADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAAYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLVISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGTDEAPSQAS"
     gene            complement(4533..5237)
                     /gene="mukE"
                     /locus_tag="OO130_002025"
     CDS             complement(4533..5237)
                     /gene="mukE"
                     /locus_tag="OO130_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HCR5365451.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEENQPDSGEEE"
     gene            complement(5218..6540)
                     /gene="mukF"
                     /locus_tag="OO130_002026"
     CDS             complement(5218..6540)
                     /gene="mukF"
                     /locus_tag="OO130_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HCR5365452.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLQVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6537..7322)
                     /gene="cmoM"
                     /locus_tag="OO130_002027"
     CDS             complement(6537..7322)
                     /gene="cmoM"
                     /locus_tag="OO130_002027"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HCR5365453.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7458..8237
                     /gene="elyC"
                     /locus_tag="OO130_002028"
     CDS             7458..8237
                     /gene="elyC"
                     /locus_tag="OO130_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HCR5365454.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTAKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(8214..9107)
                     /locus_tag="OO130_002029"
     CDS             complement(8214..9107)
                     /locus_tag="OO130_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HCR5365455.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQEHFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(9261..10007)
                     /gene="kdsB"
                     /locus_tag="OO130_002030"
     CDS             complement(9261..10007)
                     /gene="kdsB"
                     /locus_tag="OO130_002030"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HCR5365456.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(10004..10186)
                     /gene="ycaR"
                     /locus_tag="OO130_002031"
     CDS             complement(10004..10186)
                     /gene="ycaR"
                     /locus_tag="OO130_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HCR5365457.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(10238..11470)
                     /locus_tag="OO130_002032"
     CDS             complement(10238..11470)
                     /locus_tag="OO130_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaQ family DNA glycosylase"
                     /protein_id="HCR5365458.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLALEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(11507..12493)
                     /gene="lpxK"
                     /locus_tag="OO130_002033"
     CDS             complement(11507..12493)
                     /gene="lpxK"
                     /locus_tag="OO130_002033"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HCR5365459.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGSKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(12490..14238)
                     /gene="msbA"
                     /locus_tag="OO130_002034"
     CDS             complement(12490..14238)
                     /gene="msbA"
                     /locus_tag="OO130_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HCR5365460.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DSLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(14275..16547)
                     /locus_tag="OO130_002035"
                     /pseudo
     CDS             complement(14275..16547)
                     /locus_tag="OO130_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
     gene            complement(16754..17038)
                     /gene="ihfB"
                     /locus_tag="OO130_002036"
     CDS             complement(16754..17038)
                     /gene="ihfB"
                     /locus_tag="OO130_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HCR5365461.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(17198..18871)
                     /gene="rpsA"
                     /locus_tag="OO130_002037"
     CDS             complement(17198..18871)
                     /gene="rpsA"
                     /locus_tag="OO130_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HCR5365462.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(18982..19665)
                     /gene="cmk"
                     /locus_tag="OO130_002038"
     CDS             complement(18982..19665)
                     /gene="cmk"
                     /locus_tag="OO130_002038"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HCR5365463.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VATFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            complement(19838..20601)
                     /gene="ycaL"
                     /locus_tag="OO130_002039"
                     /pseudo
     CDS             complement(19838..20601)
                     /gene="ycaL"
                     /locus_tag="OO130_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
BASE COUNT         4990 a         5723 c         5035 g         4972 t
ORIGIN      
        1 tatgtgccat ttttttagtg cagaaacgga gttttcggaa aaagaaaagg cggcattgct
       61 gccgccttaa ttttaactcg cctgagaagg cgcttcgtca gttcctggaa gcgtttcagg
      121 gagttgcggc gcaaatcctc gcaggccgac gacatgaaca tgttcggtat tctggaagac
      181 tttacgcacc agtttatagg tggtgccttt ctccgggctg atattttccg gcgctgcgat
      241 gatgagctgc atttgcaaac gctcacacaa ttcaaacagc gtggcgatag aacgagcatc
      301 cagtcgcgct gcttcatcga ggaacagcag acggcaagga gagatatctt taccgcgcag
      361 gcggcgagat tcatcttccc agctttgtac caccatcacc agaatcgaca taccggtacc
      421 aatcgcctca ccggtcgaca atgcaccaga ctctgcgcgc aaccaaccat cggaaccacg
      481 gttaacctca acttccattt ccagatagtt gcggtaatcc agcagttctt cgccaatggt
      541 ctgcggcgtg cgctgcccca tatcaatctg cggattaaga cgctgataca atttcgccaa
      601 cgcttccgag aaggtcagac ggttgctgtt aaacagatcc tgatgctgct cgtgctgttc
      661 agagagcaca tccagcagca tggcgtgcgt ttcacgcacg ttcacgttga ggcgcacgct
      721 gttcacctga ccaaacgata cgttctgcaa cccctggttg agcatacgga tacggttctg
      781 ctcgcgctga atggttttgc gaatgatgtt cgccacgctg cgggaactga tcaccagttt
      841 ctgttcacgg gaggttaatt cttcggtcag acggctaagt tcgatctcca tctgttcgat
      901 agcttccacc ggatcatcgg tacgaataat atcctgacga atacgttcac gcagatgctg
      961 ataagccgcc acgaagaact gaattttacg ctccggacgt ttcggatctt ccgacatgcg
     1021 cagcacgtcg cgcagatgtt cgttatccgc caccgccaga cgcagcgcac ccaatgcctt
     1081 atccgacata gagcgcaaat catcagcgga gagataagcc agctcacgac ggtgtaagcg
     1141 gcgctcaacg ccgttatctt tcaccatgcg catcaccgca caccagcccg ctttggcggt
     1201 cactacctgc tcgcgcatct caaagtaatc ccgctccagc ttgcgcagtt tgcgggtcag
     1261 gttgtccatc tccgcttcgc agaaggtaag cgctttttcc agttgattgc ggcgtgaacg
     1321 gttattgctc agttgcgcat gcagctcgtc acggcgaata cgcgcccgct cttctgcccc
     1381 gctatcagca cacacgccga tatcctgcaa ttcacgttgc agatcgttga gtagctcttt
     1441 tttggtgtcg taagaacttt tcagcgaagc cagcacctgg ttgtactgac tcaactgcgc
     1501 tgcgtgaccg cgcaacgctt cgcgagcgcg ggtacgctcc gcttccgcct gttccagacg
     1561 ttcacgcagt ttttcgttga gatcgctgtt accgctaagc atttctgccg agtcagagta
     1621 gctaaagtgc gcacgacgct gcaccacttc cgtcagggca aacgcctgct gacgggcatc
     1681 gcgctgcatc tgttgagagt acgcgtaatc ttcttttaac tgttcgaact gttccgggtc
     1741 gctctgcaat accgaaacga tcggttccag tttcgccagt tgattgccaa actgctgaac
     1801 aaaacgcgcg gcttcctggg cttcatccag acgttcgcgg atttcatcga cgcgatccgc
     1861 caggctatca tcagccaaca ggttgagacg cggcagaatg cggttcagcg ccgtaacgcc
     1921 ctctttcgcc tgctcaaact gaatacgctg ctgctggtta tcattttcat gattacttaa
     1981 cgcccgctcc agttcgacgc gacggctgtt cagttgacgg atttctgctt ccgggtcaga
     2041 ctcaaacaca accgccagat gactgccgat aaagcggctg aacgcctgat gcagacgctg
     2101 agttttctgt acatcaaagg agagcgtggc gaagcgttcg gaaagcactt cacgctcggc
     2161 atggaggctt tcaatacggc tttcacgcgc agcacgacca aacagcggca cttccgggaa
     2221 acgtgaataa cgccactgac gatcggcgat tttcaccact accgcttttt ccagctcatc
     2281 aacgctgaac acgctgtcat cgaatgactg cggatctcct tcgatcagat agagatcttc
     2341 cgggcaatcg gtcaagccat ctaggtgttc agttacctgt gacagatctg gcaccacgat
     2401 ggcgtggcgt gacgggccat acagcgctga gaagtacggc gcatcttcca ggctaacgtc
     2461 gtcataaatt tctgacagca gcacaccacc aaaacgctcc gccagcgcgt tcagacgctg
     2521 atcttcagag ccgccaggct ggcttaaacg ttcgatctct tcatcgacgg cgtttttgcg
     2581 cgcgcccact tcatcgcgtt caacaatcgc ctctcgctca cgctccagca actgttgcag
     2641 atattcggtg acgtcctggc tggaggtaaa ctcttcgccg cactgttcgc tcaactggtt
     2701 gagactgttt tgcgctgcca gccaaaccgg cgcacgctgc atcaaactct gaatgcgaga
     2761 ctgcagctgt tcctgctcct ggcgcagtgc catgcgctct tcacgggcgt tagacacgct
     2821 atcggaaaga gaggcaatgc gtgcttccag ttcctgatgc agggcttcca gttcgtcgat
     2881 atcaaaattc ttgccctgac gtttgcagaa atctgccagc agacgctcag cttcttgctg
     2941 ctcgcgtaga cgctgttcca gttcgcttaa tcgcatccgc agcggctgaa cctgctctgc
     3001 caggtgacgc tgatcgaccc cttcgcgcaa tagttcgcga gcgacatccc acgcctcgtt
     3061 acgcgccagt gggccgttga ttgccaccac cagctgataa gcctgctcaa actggctgtg
     3121 cgcggtttgc gccatgctca ttttctgctc aagagagagc attttttcag tcgcttccag
     3181 ctctttcgcc tggaaggttt ccagccattc ggcggcgctg tcggcggtta agtccggcag
     3241 atggcacagt tctttggcac gattaagcgc agcaatcgcc tggttatact ggatcgcgcg
     3301 cgtttgctgg acgtccagcg cctgctggta gtcagcaagc tggcttttca gctcgtccac
     3361 ttccagttcg gcagcttccg cgcgagcctc gttctcttcc tggcgttcga tggcttctgc
     3421 caccacttcg ttttgctctt ccagacggat ctgcagctca tcgagatccg cttcgtagcg
     3481 ttcgatcttc tcctgctgac gcagtgcggt ttgcaccagg ttcaggtgat cgctggccgc
     3541 ctgataatcc gcttccagat cgccttcggc accgttgtgc tctgccagct cacgcgccat
     3601 atcgacgtgt ttgtactgct cagccgccag ttgctgacgc gaagtatgta actcgcgacg
     3661 aaactccagg gctttgtcga gatggacacg gcgctcgttg gcgtgacgca tgtagtccgc
     3721 cgccacgtag ttggtagctt cgctgatcag atgcttaaac aggtcacggt ccgactgggt
     3781 gacacgaatc gcttccagcg tcatgcggtt ttcacgcagc gccgcttcca tgtcctggaa
     3841 cgctttacgc acgccgctgt tttccggcaa cagatagtcg cgcagagaac gggtaatggc
     3901 actggagatc ccgccataca gcgaagcttc gatcagacga tagaatttgc tacggtcaga
     3961 tgcggagcgc agacgacgcg cgatgatgcc cagatcgaac atcagcgagt ggtaatcggt
     4021 aatggagttg aactgtttaa actgcacgcc ttccatcgcc tcgagcttgt ctttcagctc
     4081 gttaagcggc agcacgcgcg cctggcgttc gttcagggtt tcggtcacca gctgtgtcgg
     4141 ctgcaccgac atcggcagtc cctgaatggc aaatggtttg atatccactt tacgatcgcg
     4201 tccggcaacc tgttgcagac gcacaccgac caccacgcgc tggtggcgcg agttaatggt
     4261 gtcgagcatc gaataacaga cacccgcttt cagcttaccg tgcagacctt tatcgcgcga
     4321 accgctggtg gccccggctt ccgtagtgtt acggaaatgc agcagggtca ggtcggggat
     4381 cagcgccgta acgaacgccg ccatggtggt ggatttaccc gccccgttac cgccagaaag
     4441 cgtcgtgacc agctcgtcaa ggtcaaaagt tcgggcaaaa aagccgttcc agttaatcag
     4501 cgtcagtgag cgaaatttac cgcgttcaat cattattctt cctctccgct atctggctga
     4561 ttctcttcgg tttcatcgtt gagttgcaga tgattttcaa tcggcattgc ttcgccatca
     4621 cgaatcaggc gacgctgtgc ttcacgggga tcgtcgccag cacgcacatc ggctccgaag
     4681 cggaacaccg attcggtaat gcgaaacttg ctgctgtcgt ggcccataaa ccacaccatg
     4741 cctaaacgac gcagacggtt gagcgaagaa cgtactttct cctgcaactt ctgacggtca
     4801 acgtctgaac cggttgaacg gttgttcacc agtttcagca gttttgcttc atcggccagg
     4861 gtgagcagtt cgtcgtacag ttcctgctgg gtgaaaatcc cctcattcgc cagccgttcc
     4921 gggctgagat agagataaca gaggattttc ccgaccatca tatccagttc cgacaagacg
     4981 gaacgcggga taagagtggt ggaacgtggg cgtaaataga agaacccttc cggtgcgcga
     5041 ataagctcaa cgttataacg cgcgtaaaac tcttccagat attcctgaaa atccatcagg
     5101 aatgcatgat tatccagttc gtcgaggcca atatggcggc ctgaacgtaa ggcgctgtcc
     5161 agcgccggaa ataacggatt cgccagcgcc tgcgccagct taaccggcat cacttgttca
     5221 atatttgtcg atgacatgcg cctgtacctt ggctccgtaa tcattaatcg gctgccattt
     5281 cgctggcagt ccggtgaaat ctgcttgcgc tacgccaaga cgtaccgcct gatcaataac
     5341 aatacgcgca acgtcaaagt gacgtgcacg cggatactgt gacagatatt cgcgtaccac
     5401 cagaccaaga tccagcggca cttgtctggt tttgtacacg gcaagttgtt cttcgatgat
     5461 cgccgccagc tgttcgcgga tctcgttaaa ctcttcgtat tccagatcct caggaagttc
     5521 cccagtcact tcttcatcgc gcagtgccat ctcttcgtca cgcatatcca gcagacgatc
     5581 ggcattggca taagttagcg cccacggctc atcaaaatag gtttgtaccg actgacgtaa
     5641 ccgctgagca aagacgcggt ttttatccat atcgatcgcg gtacgaataa atttgtgtac
     5701 gtggcggtcg tagccaatcc acaagtcgat ggattgctgg ccccaactga taatacgatc
     5761 gagtttgctc tgcagatcga acaccagacg atcgacgaaa tgcagatcgt catgggtcat
     5821 cgtcgcatcc tgaatgcgca acagattagc ctgcaatttg tcgcctgccg cttccagcgt
     5881 atcctgcaat tcacgcagcg ttccggaagt ttccgaaagc aacaattcac agctggaaat
     5941 cgccgcccgc cagtctttgt tcagcaactg ggcgatatcg tccttcacct gctgctgctg
     6001 ttcgtccatc agacgttgcg tcaggtcgat actgtcgaaa atttctgcta ccgaatattt
     6061 cagtggcgca tagacattac ggtgccagtg aaattcatca ccgccctctt cggcggcatc
     6121 tgctgcgcgt ttgagctcac ccgccacaat cgacaactgc atagaaagac gcagcgtaga
     6181 aaactcgcgc tgacggatgt agtagtcagt aatgccgatg ccgagcggcg tcagacggta
     6241 aattgcgttc ccttccgcct gctcgctggt aaagcggttc agcagacgtt gacgcaccat
     6301 gtcgttgatc gcgttattgg cgcgcacgcc gatggtttcg ctggtttgct caaacgcatc
     6361 actcacatgg cggaatgcat ccaccagctc gccttcactc atctcaccat ccagacgctc
     6421 gccgttcagc gtggcaaccg ccagcagaaa agagagtcgg tctacctgca gcgagatgga
     6481 gaagtcattt tttctggccc aggcaaccag ttcggggact gtctgggaaa attcactcat
     6541 actttatcct tgctctgcgg tttgcgcgcg gtgacatgaa tataacgccc cagggtaata
     6601 tacggttcct gacggcaata acgcgtttct aattcaagta atgcttcata gcagtcgcgc
     6661 tgctggtgct tctcgcgcag ataatcatga aacacgcgaa cgcctgtctt acccataatt
     6721 tgccaaccag cttcttccag ccacagataa acctgcgccg ggtcgcgtgg ataatctggc
     6781 gaaagcgtcc gttttttctt tttcggcatt cccgcctgca cgtaatcaaa attcccggcg
     6841 accatgttat gcatcaacaa accatgcgca ttgtagaaca ttaacgacaa cacgccgcct
     6901 ggacgcaaca ctgaccacag ggtctgcaat acactgcggg gatcggccac ccactcgagc
     6961 accgcatgga acaatatcag atcaacgggc gtttccaaat gcgaagcaac atcctgagcg
     7021 gcgcaatgta taaattgcat gttgtcgctc acaccttttg cttctgccgc ctgttttgcg
     7081 cggtcgatca tctgcgcaga aagatcgcat aaaatgacct gatgcccacg ctcggccatt
     7141 ttgattgcgg tctgcccttc tccaccgcca gcatccagca cacgcagttt ttgcgggccc
     7201 atttccgcca gcacgcgatc gagatcctgc cacagaatag cctgtcgaag ctgccctttg
     7261 gtggtgccgt aaatgttacg ggaaaacttt tccgcaatat catcaaaatt gcgatcctgc
     7321 attgctttct ccaccggttg gctaagacgt tttctgataa agacgctatt ttgtcacagc
     7381 cacggcgaga atgaacccgt ctggtgtaat atcgctggcg atcccttgct atatggttaa
     7441 aaaaggaacc agaaaggatg ctttttacac tgaaaaaagt gattggcaat atgttgctgc
     7501 cacttccgct gatgttgtta attattggcg ctggcctggc gctgctgtgg tttagccgtt
     7561 ttcaaaaaac cgcgaagatt tttattagca ttgggtggct ggcactcttg ttattgagtt
     7621 tgcaaccggt agccgaccgc ctgctgcgtc ccatcgaaag cacctatccg acatggaata
     7681 attcgcaaaa agtggactat atcgtggtgc tcggaggtgg ttatacctgg aacccgcagt
     7741 gggcaccgag ctctaattta atcaataaca gtctgccacg cctgaatgaa ggtattcgct
     7801 tatggcgcga aaatccggga tcaaaactga tcttcacggg aggcgtagca aaaaccaata
     7861 cggtgagtac agcggaagta ggtgccagag ttgcgcaatc gctgggcgtg ccgcgcgagc
     7921 aaattatcac cctggatttg ccaaaagata ccgaagaaga agctgcggca gtgaaacagg
     7981 cgattggtga cgcccctttc ctgctggtga cctccgcatc acatctgccg cgggcgatga
     8041 tcttttttca gcaggaaggg ttaaatcctc taccagcccc ggcgaatcag ttggccatcg
     8101 actcgccgct taatccgtgg gaacgggcaa tcccttctcc ggtctggctg atgcatagcg
     8161 atcgtgttgg ctatgaaacg cttggccgca tctggcagtg gctgaaaggt ccgtcaggcg
     8221 agccacggca agagtgattt tgatgccaga tcgaagttgc gccgactaaa tcgtccggta
     8281 ttaaccagtt gcgcaacttc atcccataac acatacaacc aacgccgcca gatgaacgac
     8341 tccgccactg gcgcgcgttg caggtaactc cagagcaaat cttccgccag agaattatcc
     8401 attagtcgga acagttcgta ttctcgcggt gcccaaagca ttagtcccgg cccgaccatc
     8461 gccagtaact gatcgctgcg cgaatctttc agcatgctgc gtaaacagaa gttaccgtga
     8521 atcaacacac aattgtcgtt aaagccttca aataatgccg ggagacattc gcgagtgcga
     8581 aacaggatcc gcttatcctg catcgtcaga ccggtgttat tgaactgatt gagcgtggtc
     8641 catagcactt caacatgttg ccggtaccat gagggccaga aatgttcctg agtattgtcg
     8701 accgcgccga cgcaaccgcg actgtcctga cggtgccagg ccagtaaggc ttcaacgatt
     8761 tggtctttga gctgttccca gcgttctggt gttcgggctg gtgcctccac cgaaacacca
     8821 cgcatccgct ccagtagcag gacatcaggg ccggggtgtt cttcatgcgt catcacacca
     8881 taaatagtcg gcatacggac agtcccactt cttgccagca tggtggtttt ccatgccagt
     8941 tgtcgggctt ttccgggcgt actaaagctt cttgccatta acggcattgg gtttccctgg
     9001 ctgtcataca aagcccacaa cgccgtatcc gctttttcat tgacgcactc aatacgactg
     9061 agtttttcgc ccagtaaatg gcttaattcg gcacgcagct gttccattca gattaccctc
     9121 ataaaagcta ctgctttcat aatgagcgcc aatataaaag atgtcacctg aatgagatca
     9181 attgttgaaa agaaaaattg aggaaaatcg taacggtacg acactcctcc caaaattggc
     9241 tgaagtgtcg tgaagtgaaa ttagcgcatt tcagcgcgaa cgcgctcaag atcttcaggg
     9301 gtatccacac ctgtgccagg aacttcctga gcaacagcaa catggatttt ttcaccgtac
     9361 cacagaacac gaagctgctc taacatttcg atgtgttcta acggacttgc ctgccagttg
     9421 acgtaacgac ggataaagcc tgcacggtag ccataaatgc caagatgacg caggaagtta
     9481 tcgccaacgg tttcaaggcc ttctgcaaaa cgatcacgat cccaaggaat ggtggcgcgt
     9541 gagaagtaca gtgcataccc ttcagcgtcg agaaccactt tcaccgcatt cgggttaaac
     9601 gcttcttccg cattgtggat tggcaccgcc agagtcgcca tacccacctg acgctgagcg
     9661 aggttatcag caacctgacg aatgattgtc gcagggatca tcggttcatc accctgcaca
     9721 ttaacgatca ccgtgtcgtc gctgaatgcg catttttcga caacttccgc cagacgttct
     9781 gttcctgact gatgatcggc gcgcgtcata catacttcac cgccagcggc ttcaacggcg
     9841 cgggcaacat cctcatgatc ggttgccacg atgatgcgct cggcacctga ttcacgcgcg
     9901 cgttcaagaa catgaacaat catgggtttg ccgttaatat caaccaatgg tttaccgggc
     9961 agacgcgtgg acgcgtagcg cgcgggaata atgaccacaa aactcatgat ttactctcat
    10021 cagcagtcag cacgcgggct tccgtttcca gcaacactgg aatgccatcg cggagcggga
    10081 aagccaggtt atccagtttg caaataagct cttgtttttc ctggttatac caaagttttc
    10141 cgttgcaaac cgggcaggca atgatttcaa gcaaacgatg atccatagtt cctccagatg
    10201 gatcgggtta tgaatgcata aatcttatca taatcattta tgcgacgggg tctatttccc
    10261 agccagtgcg gcaatcagta aagagacctt gcgggcagcg tcctaatgtc acccgcgttg
    10321 cctgctgcca gctcgcgaaa tcagtaatcg cctgacgtaa ccctttttgc agcattgtcg
    10381 ttggtttaat gccttcctgt aaccacagag agataacttc aaggatgcct gtctggcgat
    10441 gcattttggc atccattcgc ccaactaatt gcccacgatg taataatggc agaacaaaat
    10501 agccatactg gcgtttcggc gctggggtat agcactccag ccggtagcta aaatcaaaaa
    10561 gctgctctgc gcgtttgcga tcccagacaa caggatcaaa aggcgaaagt accgcgctgt
    10621 gcgttgcagt gagctttccg gccagcgcac gctcgagtag cggcagcaaa tcagcatgca
    10681 gccaaagatt gcccaatttt tcaacatgca cagcaatgat ttgctgctgt tcagccctcg
    10741 cttcgcgcca cgccgccagt gccggacgtt tcagccgata gtaatctgcc agccactgtt
    10801 cgcggaatat tcccaggcta cgcgcactgt tatccagcat gatgatttct gcttctgttt
    10861 gcgaaacgag atcgcgctca tcatcccagt caggcatgac acggtgggtt aaatcataaa
    10921 cgcgctggaa gttgcgccgt tcaatcacca tcacctttcc ggcagtaaat aaaccttcca
    10981 gatgccgttt atgcggtttc cattcccacc agccgcttgc acctttacga ggatgctcaa
    11041 aatcggctga acgtaccggc cccttatcat gaatatgctg aattaactgt gcaatttccg
    11101 cctcatgttc ctgcatccag gcgtctttgt atttccagcc cattttttca agtgccagca
    11161 tgcggtggcg aataagacga aagtcgctac gcggcataaa gcaggcttca tgcgcccagt
    11221 attccattaa ttcgccacgc gccagagact catccagcca ctgggcagga taatttccca
    11281 gacgactgaa aagcaccaga tatggactac gggcaacaat attgatggta tcgatttgca
    11341 gcaaggacat gcgggagatc gttgccggaa tatcctccaa cgacgctcga cggcggggtt
    11401 tgtttaacag gccttgtgcg gcaaggtgaa gattacgcgc atcagcaagg gagaggtgcg
    11461 gcagcgacat tcatgactcc atcaatcgaa cgctgccgcg gcgtaactag ttgccagaag
    11521 ccagcgaggt tagttgcgta agcagtttcg ctggttcatc acctgaaagc tgtgcgtcta
    11581 caggcaaata ccaccaattt tcttctgcaa aggcccggca tttcaccgca tctttttcag
    11641 tcattaccag cgtttgcccg gcgcttacca acgcactgac atccgcatgg ttcaaagact
    11701 gatgatcggc cagcggtaca catttttccg gttgtacgcc acacatcttc agcgtggcaa
    11761 aaaagcgcgg cggatgccca atccccgcca tcgccactac atgttcaagc tgagcaacgt
    11821 cacaacgcgt accggtacgt aaattcaccg cctgacccgg cagcagatgc atgggaattt
    11881 caccgctgcg agggacacca ccgttgacga ttaccgcatc aaccgacttt aagcgccccg
    11941 ctcgctcacg cattggcccc gccggcaacc accagccatt gccaaagcga cgcacaccat
    12001 caataacgac aatttccaca tcacgcgcca gacggtaatg ctgtaaaccg tcgtcggtta
    12061 cgatgatctg cacatcaggg tgttgcgcca gaatggcttt taccgcatca gaacgaacgg
    12121 gagaaaccgc aacaggcgca tcagtgcgtt gataaatcaa cacaggttca tcacccgcct
    12181 gtgctgttgt ggtatctgcc gacaataaca gcggataaga ttcagcctta ctaccatatc
    12241 cccgcgatac gacccccacg cgaataccgc gctgttgcaa ctgttccacc agccagacaa
    12301 cgaccggggt ttttccgttg ccgcctgcgg tgagattacc aaccacgaca accggtacgg
    12361 gggcacgcca ggcgcgcttc agttttagtt tatagcaaag acggatcgcg ccactcacca
    12421 ggccatacaa ccaggagagt ggcagcaata gccgccacaa aggggattca ccagaccaga
    12481 ttttttcgat cattggccaa actgcatttt gtgaagttgc gcgtaaacgc cgcggtgctc
    12541 aagcaaatcg ttatgcgtac cgcgttccac aatgacacca tcctcgacga ccacgatttc
    12601 gtctgccttt tcaatggtag acaagcggtg ggcaatcacc agagaggtac ggtttttctg
    12661 caactcatcc agtgccgcct gaatcgcacg ttcggattcg gtatccagag ccgaggtagc
    12721 ttcatccaga atcagaatcg ggctatcacg cagcaaggct cgagcgatag caatacgctg
    12781 acgctgaccg ccagagagca gcacgccgtt ttcaccaatc actgtatcga gaccgttatc
    12841 catcttattg atgaagtcca tggcgtaggc catacgcgcc gcttcttcaa tttgctcacg
    12901 gctgtactgt tcagtccgtg cgtaagcaat gttgttagca accgtatcgt taaacagatg
    12961 gacattctgc gacaccagag caacctggtt acgtaacgac gccagggtat actcgcgcag
    13021 atcgtgacca tccatcagga tttcgccttc atcaatatcg taaaaacgag tgatcaggct
    13081 ggcgatggtt gatttacctg aaccagagcg tccaaccaga gcaaccgtct tccctgccgg
    13141 aattttcagg ttgatgttac gcaatgcagg tacgtcacgt cccggataag taaaggtgac
    13201 attgcggaat tccacgtcgc cagtcgcacg ctcgatcacg cgcttacctt cgtctttctc
    13261 ctgctcactg tccagaatgg taaacagcgt ctgacaagcc gccataccgc gctggaactg
    13321 ggcgttaacg ttggtcagtg atttcagcgg acgcatcagt gcaatcattg aagagaaaac
    13381 aacggtaatc gtaccagcag tcaggctatc catgacactt gggaagctcg ccgcatacag
    13441 aacaaacgcc agcgccaaag aggcgatcag ctgaatgatc ggatcagaga tggaagaggc
    13501 tgaaaccatt ttcatcccct gaagacgcat tcggttgctg actttatcaa agcgtttcgt
    13561 ttccacttcc tgaccaccga aaatcaatac ttctttgtgg cccttcagca tttgttcggc
    13621 gctggtggtc acctgcccca tggtgttctg catgttttta ctgatgttgc gaaaacgctt
    13681 cgatactacg cgaatcgcaa tcgaaacaat cggtgccagc acaatcaaaa tgatcgacag
    13741 ttgccaactg taatagaaca tcatgatgaa caggccgatg attgacgcac cttcacgtac
    13801 aaccgttatc agtgcgccgg aagaagaaga agcaacctgt tcggaatcgt aggtaatacg
    13861 tgacaacagc gtccccgttg actgtttgtc aaaaaatgaa accggcattc ccatcatgtg
    13921 accaaacagg cggcgacgca tggtcattac cacctttcct gatacccagg agatacagta
    13981 gctggagaca tagctggtga taccacgtaa aatcatcagc ccgatcacca ccagcggcat
    14041 ccacaccagc acggagcgat ctgttttacc aaagccatca tcaagaagtg gcttaaggag
    14101 cgataacatg aaggtatcgc tggctgcgtt gaggattaac gctacgcccg ccacgatcag
    14161 acccgcttta aaaggcgcaa tggttggcca cagtcggcgg aacgtctgcc acgtagagag
    14221 atctttgtcg ttatgcattc aaaaaaccag catttgttga aatagccgca tattctaccc
    14281 gttatccact ggcacgccaa accactgatg ataccaacga ggtaaaattt gctctctgag
    14341 gctgctaatc cgccagcctt gcgctgaaaa attgaccgtc acttgaccct gatgtggagt
    14401 atcaagccat tgatatcctt gctgttgata gcgatgctta actttattag agggcaatcg
    14461 ccatgcgtta tagcgttata gcgcgatgcc gatgcgagtg ccacttttcc attcactcgc
    14521 tgaattaatg gcaacgatga tgaggtatta ctgccatggt gaggtacctg aagcaatgtt
    14581 gtctgcactt gctgccagta acggcttagc atcttttgtt cagcggggac ttcaatatca
    14641 ccggttagaa gaatgctatt cgtcccgtca taaaccttaa ccacacagga atggttattt
    14701 cctttatcgt tgctagcttg taaaggccag tgcacgctga aacgcaatcc ttgccattgc
    14761 cacgcttcgc cacgcacaca gggctgatga tgttcccagt ttaacggact tctgatccat
    14821 aacatcggcc atgtgtgcaa tattgagtcc agccctcccc ggtgatccag atgttcatga
    14881 ctcagaataa cgccttccgg ttcaagatta tgccagtgga gccaggggat aatcagttgt
    14941 tgcccactat ccccttcggg ccaggccagt cctgtgtcat agagaatcgc tttgccgttt
    15001 ctggctatca ccattgccag cccttgcccg acatcaagca tgtacacctg ccactcgtca
    15061 ggtcgaggtt tttgccacag cggccagcac atcagcaagc ctacagccac acacattgct
    15121 ggcaacgttc gccaggcgtt taatcgccat accaccagta agaaccatgg ggaaaatgat
    15181 agccattgcc aacgttcagc aatgttgatc cacccttctg gcaaactctt taacccccag
    15241 aaaagtaaag ccaaagaccg gtcggcaaga aaccataacc cttgctccag gattaacggc
    15301 ccgcttaaat gcacaaccat cgcggcgagg atcaacggaa ccgtgataaa tgtcaccaag
    15361 ggaattgcta atagatttgc aataaacgag gtcagactaa tgccatgaaa tatgacgatt
    15421 tgcacaggca taagcaggag tgtgattccc agttgcaaat gaatgaggga aacaactgca
    15481 cgcaataccg gcggcagttg ccactcagga cagggaaacc actggtacca aaaaatcagt
    15541 gccgcgaccg cagcggcaga gagccataaa ctttgcgaga gaatggcaac aggatccatc
    15601 agcaaaattg ccgccagaca acatatccat acatcccagc cactccactg tcgcccactt
    15661 aacttaagca ttccccacgt agcaagcgcc accacggtac gcaatgcagg aggttgcatt
    15721 cccgtcaacc aggcataaaa agcagcacag cagattccac caattaatgg catttgccag
    15781 tggatccagc gcccaggcag aaaaacttgc ccaccgcgaa tgagtccggc agccagcaac
    15841 gccgcaaaag cgatgtgcaa tcccgatatc gccattaaat gcgccgttcc agtatcacgc
    15901 atgatatttt tgatttcttt ggggacggat aaccgttccc ccatacctaa accaagaata
    15961 accgcattcc acatataggg ttgcagcgtt gtttgtagtg acgccagata ctgtgcacgc
    16021 aggctacaat tcggttcaat gacacttgcc tgcagaaagc ggccgatgag cggctgatgc
    16081 tgggcaatgg cataacgctg gctatcaaag ccgccatcat taagttggcc atgaactgca
    16141 cgaactttga gtttcattga ccattgttgt ccagcacaaa ccgcttgcgg aagatattcg
    16201 ccatacagca caagacctgg cgcagggaat atacgtcgac cttgtagatg agtaatttga
    16261 ccgtaatgag tggtcatgcc gtcagttgca gtgatctcaa caattgcatc ctgcgtcgcg
    16321 ccagttaagg tttctcctgc ccacaaaatt tgctttgctg ataatatgcc ccaaacgaaa
    16381 aacagcaacg tcagcgcgat ataacggacg gttttaacag ggataaatgc cagtacgcag
    16441 gcgaagagag tcagaaacgc aagggttaat gtcccaggca attggggcaa aatcagcaac
    16501 ggaaaaattc cgcaaattat gcatacgccg actgtcgtta ttttcatgca cccctccctg
    16561 tttatggaaa gtgtgcaact ttgttgagcg gttgtctggt gggaattttg cgttccccga
    16621 ggcacattca gaagaattta acgaaagggt gaaaactgct caaaactctg cgaggcagct
    16681 tccagttcct gattagagaa atgaaaaaag cacccgacag gtgcttttct cgcgttcaag
    16741 tttgagtaaa aacttaaccg taaatattgg cgcgatcgcg cagttcttta ccaggtttaa
    16801 agtgaggaac gtattttcct tccagttcta ctttatcgcc agtcttcgga ttacgtccgg
    16861 tacgtggtgc gcggtagtgc aaagagaaac tgccgaaacc gcggatttca atacgctcgc
    16921 cctgcgcaag agtcgaggcc atatgctcca gcatctcttt tactgcatct tcaaccgtct
    16981 tggcgggaat gtgcgattgc tgggtggcaa gtctttctat caattctgac ttggtcatga
    17041 ttcctccggt tccttaaagg caaattaagc ggctgctgtt gctgcattga ttaagggcgg
    17101 ccgtagccgc ccttagtgct tgattacagg acgaaacctg caatctgtca agtaaactca
    17161 acaaacttcg gaataaaaat cccgaagagt cagagaatta ctcgccttta gctgctttga
    17221 aagcttcagc cattgcgttg ttggagaagt ttgcatcttc ctgtttgtta acagttgcga
    17281 ttgcatcttt ctcgtcagct tcgtctttcg cacgaacaga caggctgatt gcgcggtttt
    17341 tacgatcaac gccggtgaat ttagcttcaa cttcgtcgcc aacgctcaga accagggtag
    17401 cgtcttcaac gcggtcacgg gatgcttcag aagcacgcag gtaaccttca acgccgtcag
    17461 ccagttctac ggttgcgcct ttagcgtcaa ctgcagttac tttaccggtt acgatagcgc
    17521 ctttcttgtt cagagcaacc cagttgttga acggatcttc tgcgagctgt ttaacgccca
    17581 aggagatacg ttcacgttct gcgtcaacct gcagaacaac tgcagcgatt tcgtcgcctt
    17641 ttttgtattc acgaactgct tcttcgcctg caacgttcca ggagatgtca gacaggtgaa
    17701 ccaggccgtc gatgccgccg tctaggccga tgaagatacc gaagtcagtg atagacttga
    17761 ttttaccttc aacacggtcg cccttgttgt gggtttccgc gaactgctgc cacgggttag
    17821 ctttgcactg tttcagaccc agggagatac gacgacgttc ttcgtcgata tccagaacca
    17881 taacttccac tacatcgcca acgttaacaa ctttggacgg gtggatgttt ttgttggtcc
    17941 aatccatttc ggaaacgtgt accaggcctt caacgccttc ttcgatttca acgaagcagc
    18001 cgtagtcggt caggttggtc acgcgaccag tcagtttggt accttccgga taacgtttag
    18061 cgatagctac ccacggatct tcgcccaact gtttcaggcc cagggataca cgggtacgtt
    18121 cgcggtcgaa cttcagcact ttaacagtga tttcgtcgcc cacgttgacg atttcgctcg
    18181 gatgcttaac gcgtttccag gccatgtcag tgatgtgcag caggccgtca acgccgccca
    18241 gatcaacgaa tgcaccgtag tcagtgaggt tcttaacgat acctttaact tccatgcctt
    18301 cctgcaggtt ttccagcagc tgatcgcgct ctgcgctgtt ttcggattcg ataacggcac
    18361 gacgagaaac aacaacgttg ttgcgcttct gatccagctt gattacttta aattcaagct
    18421 ctttgccttc caggtgcaga gtgtcacgca ccggacgaac gtctaccaga gaacctggca
    18481 ggaacgcacg aataccgttc agctcaacag tgaagccgcc cttaactttg ccgttgataa
    18541 caccggtaac agtttcagca tcttcgtaag ctttttccag cgtgatccag gcttcgtgac
    18601 gtttagcttt ctcacgggac agcagagttt caccgaagcc gtcttctact gcgtccagag
    18661 caacgtcaac ttcgtcacct acctggattt ccagctcgcc ctgggcgttt ttgaactgct
    18721 cagccgggat ggcggactca gatttcagac cagcgtcaac cagtactacg tctttgtcga
    18781 tagcaacaac aacgccacga acgatagaac ccgggcgggt ttcgatttct tttaaggact
    18841 cttcaaagag ttgagcaaaa gattcagtca tgtttaatct tcaggtttat atttaacgtc
    18901 cacctggctc catgccggat ggggttgttt aacatacccg ctgtcattcc attgcaacgg
    18961 gggtactgca aattcggtcg cttatgcgag agccaatttc tggcgcgcgt attgtagcgc
    19021 tttttcaatc acttgctcaa tgcttaaggt ggtggaatcc aacactaaag catcggctgc
    19081 cggaaccagt ggcgctaccg ctcggttacg atcgcggtcg tcgcgttctt tgatctcggc
    19141 caaaaggcgc tcaaagttaa cactaaagcc cttctcctgc aactgtagca tgcggcgatg
    19201 cgcacgttct tccgaggagg cgtcaaggaa aattttcact ggtgcatcag ggaataccac
    19261 cgttcccatg tcgcggccat cggcaatcag acctggtaat tcgcgaaacg cgcgttggcg
    19321 acgcaataat gcttcacgaa cgcgtgggaa tgtcgcgact tgtgaagccg cattcgccac
    19381 ttcctgagta cgaatttcgc cgctgacatc ttccccttcg aggatcactt ccagattgcc
    19441 attggttgac acaaaacgta catccagatg ggatgccagc ggtaccagcg catcttctga
    19501 cgcaacatca acatggtgat gtaatgccgc caatgccagt acgcgataaa ttgcacccga
    19561 atccagcaga tgccattgca atgcttccgc catagcctta cacaaggtgc ctttccctgc
    19621 accgcttggg ccatcaatgg taataaccgg ggcaattgcc gtcatcttta tctccttaaa
    19681 acaggcgtac cgttaacata accgcgcgca ttatacgcgc cgacgccaca aatcgttaac
    19741 gttgtgtgca aaaaccagtt ttgtctgtat tcagatgggg atttattgcg caattatagc
    19801 gggctggcag aagaccagcc cgaaagatga caatgattta cttaccagag gcgatacgat
    19861 cacggatgtg ttgcgcacgc tctgttgatg gtgggtgaga gtcaaacatg gactgggtgc
    19921 gaccgccatc cagactagcc agtttttcaa agctgccaac cagcccctag gtgctgatgc
    19981 cacgtttctt caacagatca aaggagaaat catctgcttc ggactcttta tcacgggagt
    20041 atttagcatt aatagcgcct tctgcgatat cacccaattg tgagcgggaa agctgggaag
    20101 ccacaccgct ggtagctgaa atggcatcgc gtgcggcaac gatcgcataa gaaactttca
    20161 tttcagccag cgagtgaccc aacgcgacgt ggcccagttc atggcccaga acaccttcaa
    20221 tttcgttgtc attcatcatg tccatcaggc cactgtagac acggacacag ccgttcgcca
    20281 tcgcccatgc gttgacgtcg ctggtcatat aaacctatag ttgaccggcg tgccattaat
    20341 attgttaccc aatgctttgg cgatttttgc cagacgttta ccgtacttgc tggatttact
    20401 tgcgacttga ttgccgctgt ccatttcggc acagccctga ttggcaatcg cttttgcatc
    20461 ggcatcgctt aatgttgctg ctttataggc atttaaaccg gagctgatag ccatattggt
    20521 atcaataccg tgggtatttt gacaccctgt cagtaatgct gcagaggtcg caatcgccag
    20581 cagtaattta gtattcttca tgtgaatcat ccgttgatta atcatatata aacattatgt
    20641 atcaacaact tacattgaca agcgacgcta ccattaatca aaatgactca caaggtccga
    20701 aaaaaaacgc cgacaatgtg
//
LOCUS       DAJTMC010000063        20698 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.063, whole genome shotgun sequence.
ACCESSION   DAJTMC010000063 DAJTMC010000000
VERSION     DAJTMC010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20698
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.063"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(<1..224)
                     /gene="hyfA"
                     /locus_tag="OO130_002040"
     CDS             complement(<1..224)
                     /gene="hyfA"
                     /locus_tag="OO130_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HCR5365464.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     ITAPVVCHHCEEAPCLQVCPVNAISQRDDA"
     gene            complement(477..947)
                     /gene="bcp"
                     /locus_tag="OO130_002041"
     CDS             complement(477..947)
                     /gene="bcp"
                     /locus_tag="OO130_002041"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HCR5365465.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(947..1519)
                     /locus_tag="OO130_002042"
     CDS             complement(947..1519)
                     /locus_tag="OO130_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HCR5365466.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            1665..2543
                     /gene="dapA"
                     /locus_tag="OO130_002043"
     CDS             1665..2543
                     /gene="dapA"
                     /locus_tag="OO130_002043"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HCR5365467.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            2560..3594
                     /gene="bamC"
                     /locus_tag="OO130_002044"
     CDS             2560..3594
                     /gene="bamC"
                     /locus_tag="OO130_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HCR5365468.1"
                     /translation="MAYSVQKSRQAKVAGVSLILLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGVRTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTIDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSASDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            3807..4520
                     /gene="purC"
                     /locus_tag="OO130_002045"
     CDS             3807..4520
                     /gene="purC"
                     /locus_tag="OO130_002045"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HCR5365469.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKRFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            4688..5551
                     /locus_tag="OO130_002046"
     CDS             4688..5551
                     /locus_tag="OO130_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HCR5365470.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHNMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            5566..7581
                     /gene="tmcA"
                     /locus_tag="OO130_002047"
     CDS             5566..7581
                     /gene="tmcA"
                     /locus_tag="OO130_002047"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HCR5365471.1"
                     /translation="MAELTALYTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALL
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFDDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQYFLQAAGENEIAGALWLVDEGGLSQQLSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGVGQQLIASALQYTQDLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGETLPVD
                     PLNDIVLSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNASDAQ
                     LCTTLKLAGRKMLLVRQREEAAHALFALNDVRTERLRDRITQWQFFH"
     gene            7655..8353
                     /gene="ypfH"
                     /locus_tag="OO130_002048"
     CDS             7655..8353
                     /gene="ypfH"
                     /locus_tag="OO130_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR5365472.1"
                     /translation="MKHDHFVVQSPDKPVQQLLLLFLGVGDNSVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(8463..8663)
                     /locus_tag="OO130_002049"
     CDS             complement(8463..8663)
                     /locus_tag="OO130_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HCR5365473.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(8691..9818)
                     /gene="dapE"
                     /locus_tag="OO130_002050"
     CDS             complement(8691..9818)
                     /gene="dapE"
                     /locus_tag="OO130_002050"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HCR5365474.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(9822..10178)
                     /locus_tag="OO130_002051"
     CDS             complement(9822..10178)
                     /locus_tag="OO130_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HCR5365475.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPSIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            10559..10618
                     /gene="ypfM"
                     /locus_tag="OO130_002052"
     CDS             10559..10618
                     /gene="ypfM"
                     /locus_tag="OO130_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HCR5365476.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            complement(10717..13829)
                     /gene="acrD"
                     /locus_tag="OO130_002053"
                     /pseudo
     CDS             complement(10717..13829)
                     /gene="acrD"
                     /locus_tag="OO130_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
     gene            complement(13992..15692)
                     /gene="narQ"
                     /locus_tag="OO130_002054"
     CDS             complement(13992..15692)
                     /gene="narQ"
                     /locus_tag="OO130_002054"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HCR5365477.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSPVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYLSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGENWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQHLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            15898..17877
                     /gene="aegA"
                     /locus_tag="OO130_002055"
                     /pseudo
     CDS             15898..17877
                     /gene="aegA"
                     /locus_tag="OO130_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416963.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
     gene            17945..18520
                     /gene="nudK"
                     /locus_tag="OO130_002056"
     CDS             17945..18520
                     /gene="nudK"
                     /locus_tag="OO130_002056"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HCR5365478.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKTVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQTSHLMD"
     gene            18646..19689
                     /locus_tag="OO130_002057"
     CDS             18646..19689
                     /locus_tag="OO130_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HCR5365479.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPEASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKNPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(19799..20698)
                     /locus_tag="OO130_002058"
                     /pseudo
     CDS             complement(19799..20698)
                     /locus_tag="OO130_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311354.1"
                     /note="catalyzes the formation of ribose 5-phosphate and
                     xylulose 5-phosphate from sedoheptulose 7-phosphate and
                     glyceraldehyde 3-phosphate; can transfer ketol groups
                     between several groups; in Escherichia coli there are two
                     tkt genes, tktA expressed during exponential growth and
                     the tktB during stationary phase; frameshifted;
                     incomplete; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transketolase"
BASE COUNT         5003 a         5294 c         5540 g         4861 t
ORIGIN      
        1 atcgcatcat ccctctggga gatggcattg accgggcaga cctgcaggca aggggcttcc
       61 tcacagtgat gacacacgac aggggcagtg attgttgacg tcttcgccag ggccaggcgc
      121 gggtgttgct gtaaaccttg cgttttatgc acgtccgaac aggcagcgag acaggtatta
      181 catcctgtac accacagtgg ttcggccacc acaaagcggt tcatgtcggc tccttgaaaa
      241 tgatatgaaa cattgcattt catattgcat aaaggatgcc agtctgatta atttaatata
      301 ttcaataaat tatgtattaa cagggttctg aacggtacaa aaatgatggt cattaacgtt
      361 cagcaaaaaa tggattcgac acccgtgtcg caggggaaag cgagaatgtg atcttgatca
      421 cgagaagtga ggtgtgctga ccgcaagcgc agccagcacg gaatggagca aagtaatcag
      481 gcgtgttctt tcagccagtt cagcacaacg tcgtggtgat tgctggtttt aaaatcgtca
      541 aagacatgtt cgattttgcc atcagcgtca atcaggaagc tgatgcgatg aataccatcg
      601 taggttttgc ccatgaagga cttttcaccc cagacgccga attgttcgca cacctggtgg
      661 tcctcatcag acaggagcgt aaagttaagc agctcttttt ccgcaaaacg ggagagtttt
      721 tcgggtttat cggtgctgat acccagcaca tcaacgcccg cttttttcaa ctcatccatg
      781 ttatcgcgta agccgcaggc ctgtacggta cagccggggg tcatggcttt cgggtagaaa
      841 taaaccagaa cacgctgtcc ctggaagtcg gtcaaattaa cttgttctcc gtcttgatcc
      901 ggcaagctaa atttcggtgc gatatcaccg gctttcagtg gattcattac ttaactccat
      961 cctgttcatc atgttgggaa taattgacga cgttaatact gccttgtgca ttgagttctg
     1021 tacatagggc tttgaacgct tgctcaatat ttgctgcgtc cgcagatgcg gggctgtggg
     1081 cggtgatctg aatatgcaac tgcgcagccc gttcattttc agcaggttgc gtgcgcgaca
     1141 ccagctccgc aatgttcata tgatgcgcgt cgaaaagtgc tgtgaagcgt tcaattaaat
     1201 gcggggagtc tgcaacatcg acctgaaccc agacagatgc tggcattggc ggacgcggac
     1261 gcgccgtcgt gcgcttcatc acgattaaaa gatccagttc ggcacctttc aacggtaacg
     1321 ttgattcaat cagagtaatg gcattccatg aaccggaaag cagcatgata aacgtgaact
     1381 cttctcccag catcgccagg cgactgtctt caatattaca gccgcaacta ctgacatgac
     1441 gggtgatggt gttcacaatt ccagggcgat cggcacccaa cgcagtgatc accagataat
     1501 gttgcgatga cagtgtcaaa ctggttattc ctttaagggg tgagttgttc ttaaggaaag
     1561 cataaaaaaa acatgcatac aacaatcaga acggttctgt ctgcttgctt ttaatgccat
     1621 accaaacgta ccattgagac acttgtttgc acagaggatg gcccatgttc acgggaagta
     1681 ttgtcgcgat tgttactccg atggatgaaa aaggtaatgt ctgtcgggct agcttgaaaa
     1741 aactgattga ttatcatgtc gccagcggta cttcggcgat cgtttctgtt ggcaccactg
     1801 gcgagtccgc taccttaaat catgacgaac atgctgatgt ggtgatgatg acgctggagc
     1861 tggctgacgg gcgcattccg gtgattgccg ggaccggtgc taacgctact gcggaagcca
     1921 ttagcctgac gcagcgcttc aatgacagcg gtatcgtcgg ctgcctgacg gtaacccctt
     1981 actacaatcg tccgtcgcaa gaaggtttgt atcagcattt caaagccatc gctgaacata
     2041 ctgacctgcc gcaaattctg tataatgtgc cgtcccgtac tggctgcgat ctgctcccgg
     2101 aaacggtggg ccgtctggcg aaagtaaaaa atattatcgg aatcaaagag gcaacaggga
     2161 acttaacgcg tgtaaaccag atcaaagagc tggtttcaga tgattttgtt ctgctgagcg
     2221 gcgatgatgc gagcgcgctg gacttcatgc aattaggcgg tcatggggtt atttccgtta
     2281 cggctaacgt cgcagcgcgt gatatggccc agatgtgcaa actggcagca gaagggcatt
     2341 ttgccgaggc acgcgttatt aatcagcgtc tgatgccatt acacaacaaa ctatttgtcg
     2401 aacccaatcc aatcccggtg aaatgggcat gtaaggaact gggtcttgtg gcgaccgata
     2461 cgctgcgcct gccaatgaca ccaatcaccg acagtggtcg tgagacggtc agagcggcgc
     2521 ttaagcatgc cggtttgctg taaagtttag ggagatttga tggcttactc tgttcaaaag
     2581 tcgcgccagg caaaggttgc gggtgtttcg cttattttat tactcgctgc ctgtagttct
     2641 gactcacgct ataagcgtca ggtcagtggt gatgaagcct acctggaagc ggcaccgctt
     2701 gcggagcttc atgccccggc tggaatgatt ttgccggtga cctccggtga ttatgcaatc
     2761 ccggtgacca acggtagtgg tgctgtcggt aaggcgctgg acattcgtcc accagcccag
     2821 ccgctggcac tggtttctgg cgtgcgtacc cagttcacgg gcgataccgc ttcattgctg
     2881 gtggaaaatg gtcgtggcaa tactctgtgg ccgcaggtgg ttagcgtgct gcaggcgaaa
     2941 aactacacca tcacccaacg tgatgatgct ggtcagacac tgaccatcga ttgggtacaa
     3001 tggaaccgtc tggacgaaga cgagcagtat cgtggtcgtt atcaaatctc tgttaagccg
     3061 cagggttatc agcaggcggt tacggttaaa ctgctgaacc tggaacaggc gggcaaaccg
     3121 gttgcagacg cggcttccat gcagcgttac agcacggaga tgatgaacgt tatttccgcc
     3181 ggtctggata aatctgcctc tgacgccgcg aacgctgcgc aaaatcgtgc ctccaccact
     3241 atggacgtac aaagtgcagc tgatgacacc ggtttaccga tgctggtcgt acgcgggccg
     3301 ttcaatgtgg tttggcaacg tctgccagcg gcgctggaaa aagtgggcat gaaagtgacc
     3361 gacagcaccc gttcgcaggg taacatggcc gtaacttata agccgctgtc tgacagcgac
     3421 tggcaggaac tgggcgcgag cgatccaggc ctggcatccg gtgactataa actgcaggtt
     3481 ggcgatttag ataaccgcag cagcctacag ttcatcgatc cgaaaggtca tactctgact
     3541 cagagtcaga acgacgcgct ggtagctgtc ttccaggctg cgtttagcaa gtaaaaatac
     3601 agggctggag tcatccggcc cttttttctg atatgatacg caaacgtgtg cgtcggcaga
     3661 aaaatgcaat tttagcggta attcgcacga aatttgtttg tcggacgtag gtcggataag
     3721 gcgttcttgc cgcatccgac aaaacatccg gcacaccaga cagcaaaaga ttttaaaacg
     3781 ttaattcaca cccaggagtg ataaagatgc aaaagcaagc tgagttgtat cgtggtaaag
     3841 cgaaaaccgt atacagcacg gaaaacccgg acctgttggt gctcgaattc cgcaatgata
     3901 cgtcagcagg ggatggcgcg cgcattgagc agtttgatcg caaaggtatg gtgaacaaca
     3961 agttcaacta cttcattatg agcaaactgg ctgaagcggg tatcccgact caaatggagc
     4021 gtctgctctc cgataccgaa tgtctggtga aaaagctgga tatggtgccg gttgagtgtg
     4081 tcgtgcgtaa ccgtgctgct ggctctctgg tgaaacgtct tggaatcgaa gaaggtattg
     4141 agctgaaccc gccgctgttc gatctgttcc tgaaaaacga tgccatgcac gatccgatgg
     4201 tcaacgaatc ttactgcgaa acctttggct gggtgagcaa agagaacctg gcgcgtatga
     4261 aagagctgac ctacaaagcg aacgacgtgc tgaaaaaacg gttcgatgat gctggtctga
     4321 ttctggtcga cttcaagctg gagtttggtc tgtacaaagg tgaagtggta ctgggcgatg
     4381 agttctcccc ggacggcagc cgcctgtggg acaaagaaac actggagaaa atggacaaag
     4441 accgtttccg ccagagcctc ggtggcctga tcgaagccta tgaagccgtc gcccgccgcc
     4501 tgggtgtaca gctggactga tttttctgtt catcatcttg ccgtgcagct ggcacggcaa
     4561 gacaaccgct ctcggaatat atgcattgaa cgctcattcc tccgccattt catcccgctt
     4621 tcctgtggta atcttcctct gaaccaccta cgatcatttc tataatgaat atattgttga
     4681 ggtatctatg cgttggcaag ggcgacgtga aagtgacaat gttgaagaca ggcgcaacag
     4741 ctccggtggt ccgtctatgg gcggtcccgg ttttcgcctg ccaagcggta aaggcgggct
     4801 gattttactg atagtcgtgc tggttgcagg ctactatggt gttgatttaa ccgggttaat
     4861 gactggacag ccggtttccc aacaacaatc aacgcggtcc attagcccta atgaagacga
     4921 agctgcaaaa ttcacctcgg tgattctggc aaccacggaa gacacctggg gacaacagtt
     4981 cgagaagatg ggtaagacct atcagcaacc gaagctggtc atgtaccgtg gaatgacgcg
     5041 caccggctgc ggggcgggcc agtccattat ggggccgttc tattgcccgg cggatggcac
     5101 agtttatatc gatctctcct tctatgatga catgaaagac aaacttggcg cggatggcga
     5161 ttttgcccag gggtacgtta tcgcccatga agtcggtcat catgtgcaga aactgttagg
     5221 catcgagccg aaagttcgtc aactgcaaca aaacgcgacg caggcggaag tgaaccgctt
     5281 atctgtgcgt atggaactcc aggccgactg ttttgccggt gtctgggggc ataacatgca
     5341 gcagcaaggc gttctggaaa ccggcgatct ggaagaggcg ctgaatgcgg cgcaggccat
     5401 cggcgatgac cgtttacaac agcaaagtca ggggcgagta gtaccagaca gtttcacaca
     5461 tggcacttct cagcaacgct acagctggtt taaacgtggt ttcgacagcg gcgatccggc
     5521 acaatgcaat acttttggta aaagcattta atttccgggg cagggatggc tgaactgact
     5581 gcgctttaca cattaacagc gcaaatgaaa cgtgaaggga tccgccgctt gctggtgttg
     5641 agcggggaag agggttggtg ttttgatcat gcgcttaagt tgcgtgatgc cttacttggc
     5701 gactggctgt ggatttcgcc gcagccagat gctgaaaacc actgttctcc ctcggcgcta
     5761 caaactttac ttgggcgcga gttccggcat gcggtattcg acgcccgcca cggctttgat
     5821 gccgctgcct ttgcggcact tagcggaacg ttgaaagcgg gaagctggct ggttttgtta
     5881 ctccctgtat gggaagagtg ggaaaaccaa cctgatgccg actcgctgcg ctggagtgat
     5941 tgccctgacc ctattgcgac gccgcatttt gtccagcatc tcaaacgcgt acttacggcg
     6001 gataacgacg ctattctctg gcggcaaaac cagccgttct cgttggcgca ttttactccc
     6061 cgtactgact ggcaccccgc gaccggcgca ccacaaccag aacaacagca actcttacag
     6121 cagctactga ccatgccgcc gggcgtggca gcggtaacgg ctgcgcgtgg gcgcggtaag
     6181 tcggcgctgg cagggcaact catttctcgt attgcgggca gtgcgattgt caccgcgccc
     6241 gcaaaagcgg caacggatgt actggcacaa tttgcgggcg agaagtttcg ctttattgcg
     6301 ccggatgcct tgttagccag cgatgagcaa gccgactggc tggtggtcga tgaagccgca
     6361 gccatacctg cgccgttgtt gcatcaactg gtatcgcgtt ttcctcgaac gttgttaacc
     6421 actacggtgc agggctacga aggcaccgga cgtggttttt tgctgaaatt ttgcgctcgc
     6481 tttccgcatt tacaccgttt tgaactgcaa cagccgatcc gctgggcaca gggatgcccg
     6541 ctggaaaaaa tggttagtga ggcactggtt tttgacgatg aaaacttcac ccatacacca
     6601 caaggcaata tcgtcatttc cgcatttgaa caaacgttat ggcgaagcga gccagaaacg
     6661 ccgttaaagg tttatcagtt attgtctggt gcgcactacc ggacttcgcc gctggattta
     6721 cgccggatga tggatgcacc agggcaatat tttttacagg cggctggcga aaacgagatt
     6781 gccggagcgc tgtggctggt ggatgagggg ggattatctc aacaactcag tcaggcggta
     6841 tgggcaggtt ttcgtcgccc gcggggtaat ctggtggccc agtcgctggc ggcgcacggt
     6901 agcaatccac tggcagcgac attgcgtgga cggcgggtca gccggatagc agttcatccg
     6961 gctcgtcagc gcgaaggcgt tgggcaacag ctcattgcca gcgctttgca atatacgcaa
     7021 gacctcgact atctttcggt gagttttggt tacaccgggg agttatggcg tttctggcaa
     7081 cgctgcggtt ttgtgctggt acgaatgggt aatcatcgtg aagccagcag tggttgctat
     7141 acggcgatgg cgctgttacc gatgagtgat gcgggtaaac agctggctga acgtgagcat
     7201 taccgtttac gtcgcgatgc gcaagctctc gcgcagtgga atggcgaaac acttcctgtt
     7261 gatccactaa acgatatcgt gctttctgac gacgactggc ttgaactggc cggttttgct
     7321 tttgctcatc gtccgctatt aacgtcgtta ggttgcttat tgcgtctgct acaaaccagt
     7381 gaactggcat taccggcgct gcgtgggcgt ttacagaaaa atgccagtga tgcgcagtta
     7441 tgtaccacac ttaaacttgc aggccgcaag atgttactgg tccgtcagcg ggaagaggcc
     7501 gcgcatgcgc tgttcgcact taatgatgtt cgcactgagc gtctgcgcga tcgcataacg
     7561 caatggcaat tttttcactg actccttcaa ttttctccca tggtcattgt gagccagcgg
     7621 cgtaaaatta atgacatcag ttaaggagtt cgccatgaaa catgaccatt ttgttgttca
     7681 aagcccggat aaacctgtgc aacagttgtt actgcttttc ctcggtgtcg gggataactc
     7741 ggtggcaatg ggggaaatag gcagttggtt tgcaccgctg tttcctgatg cgctggtggt
     7801 gagtgtcggt ggcgcggagc cgagcggtaa tccggcgggg cgtcagtggt tttcggtgca
     7861 gggtattacg gaagataatc gccaggcgcg tgtggatgcc atcatgccga cgtttattga
     7921 aacggtacgc tactggcaga aacagagtgg agtaggggct aacgccacgg cgttaatcgg
     7981 tttctctcaa ggcgcgatta tggcgctgga gagtattaaa gccgaaccgg gccttgcttc
     8041 acgcgtcatt gcttttaacg gacgctatgc gagcctgccg gaaaccgcgt ctacagcaac
     8101 gacaattcac ctgattcatg gcggtgaaga tccggttatc gatctggcgc atgcggttgc
     8161 ggcgcaggaa gcgttaatca gtgccggtgg tgacgtgacg ctggatatcg tggaggattt
     8221 aggccatgcg atcgacaatc gcagtatgca atttgccctg gatcacttgc gttataccat
     8281 tccgaaacat tatttcgacg aagcgctaag tggcggtaag cctggcgatg atgatgtgat
     8341 tgaaatgatg taaaagacag attacctggc ctgataagac gcgtgagcgt cgcatcaggc
     8401 atcgttcacc attgccggat gcggcgttaa cgccttatcc ggcctacggt tgagcactca
     8461 gcttacttct tcggttgatc ctttttcggc cagtcgtcgt catcgtccca cttatcgttg
     8521 aaatcacgat gcgggggaag ctccggcttg ttggcgagaa atttcttgtg gtcgacgcgc
     8581 ttgagatctt taatcacgtt cagcaggacg cctaccaaaa agacaatcac cagaatccac
     8641 caatatttag ccagccagtc catgcttatt tcctcttgca gaaccactca tcaggcgacg
     8701 agctgttcca tgatacgttg atacatacgg gcaagtagct gcaggtcggc agcgttcaca
     8761 cattcattaa ttttatgaat agtggcattg accggcccga gttccaccac ctgcgccccc
     8821 atgcgagcaa taaagcgccc gtcggacgtt ccgcctgtgg tcagtagctg cggtttaatt
     8881 tcattatagt gctcaaccgc gttaacgacc gcatccacca gtttaccgcg cgcggtcaaa
     8941 aatggctgcc cggaaagcca ccaatccacc gtatagcgca gttgatgttt ttcaagcagg
     9001 gcaagcacct gcgctttgat catctcatca gtcagttcgg tgctgaagcg gaagttaaac
     9061 tgcacaaaca gttcacccgg aataacattg ttactgcccg ttcccgcctg aatattagca
     9121 atctgcatac tggtcgccgg gaaaaattca ttgccctgat cccactcaat agccactaat
     9181 tcattaagga aaggtgctgc gcgatgtact ggattgtcag ccagatgcgg gtaggccaca
     9241 tgcccctgaa cgccatgaat ggtaaggttg caggttaacg atccgcgacg accatttttc
     9301 accacatcac caaccacttc gatactcgac ggttcgccga ccaggcagta atcgagacgc
     9361 tcattacgtg ccatcaacgc ttcgacgact tttaccgtac cgttgtgggc actggcttct
     9421 tcatcagagg tgatcagaaa tgccagtcgc cccgtatggt tgggatgttg tgcgacaaag
     9481 cgttctgccg ctaccaccat cgccgccagc gagcctttca tatctgccgc accgcgcccg
     9541 aataacatgc catcacgaat ggtgggttca aacggcggat tgatccaacg atcggcgtcg
     9601 ccaggcggca ccacgtcggt atgcccggca aaggctaacg tttcaccttg cccacgccat
     9661 gcccaaaaat tctgcgtatc ggcaaagtcc atgcgttcaa cggtaaaacc gatcgcctgc
     9721 aaacgttcaa tcaacaaagc ctggcatcct gcgtcatcag gactcaggga agggcggcga
     9781 ataagctgtt gtgtcagctc aataaccggg cacgacatag actacacctc atggaaaaat
     9841 tgctgataac tggaatcact gaaacccagc agcataggct taccaggcgc gcagagcaat
     9901 ggacgtttga taattgaagg catttcagtc attaatgccg ccgcagaggc cgcatcggtg
     9961 attttattgc gggtggtttc gtccagttta cgccaggttg taccacgggt gttcagtaac
    10021 gcttcccagc ctaattcgtt gataaaaccg ttcaataatt cgctgtccag cccatcgacg
    10081 cggtaatcat gaaaacgata gtcgatgtta ttggcttcca gccaacggcg agccttttta
    10141 atggtgtcac aatttttgat gccgtaaagt gtaaccataa tgaatccttt ttcgatctat
    10201 gagaataaat actcatttaa gggtgaaaac attttgcctg caaatgatta tcaggaaata
    10261 taacattatc gaagtattca aattaacaac tattaaataa tattaaacat ttttaacgta
    10321 aacattaaga atattcatct tgttatctgt ctgtatttta accagaatta aatcagtatt
    10381 agatgccgta tgaatatctc gaaatgttgc tgtgtatcac atacaaatta tcaccgcagg
    10441 accatagtat ttacataagg tttgtccccc ggaggtagtc atcgcagcaa tcgagtaaat
    10501 tagcttcacc acggtatatt gtttccgtag aatacccata attataagag aggttgttat
    10561 gattgaacgt gaactgggga actggaaaga ctttatcgaa gttatgcttc gtaagtaatt
    10621 cctgagacaa agtacaaaca gcaagaaccc gctgagcagg ttcttaatcg gaaagttgaa
    10681 aaaggcgaca cattagcatg tcgccttttt tattgcttat tccgggcgcg gcttcagcgg
    10741 gaagcggcgg cgcaccagta caaagaacag cggcacgaag taaatagcca gaatagtggc
    10801 cgaaatcatc ccgcccatta cgccagtacc caccgcatgc tgaccaccgg aaccggcacc
    10861 cgtgctggtt gccattggca atacgccgaa gataaatgcc agcgaggtca tcagaatcgg
    10921 gcgtaaacgc tgacggcagg cgtggagcgt cgcttcaaac aggtcgtggc ctttttggtt
    10981 catctcgtta gcaaactcga cgatcaggat ggcgtttttc gccgataaac caatgaccgt
    11041 taacaggccc acctggaagt aaacgtcgtt ttccagcccg cgcatccagg ttgccagcag
    11101 cgcgccgata acccccagcg gcacgaccag cattaccgag aacggcaccg accagctttc
    11161 atacaacgcc gccagacaca ggaataccac cagcaaggaa atggcgtaca gcgccggagc
    11221 ctgcgcgccg gaaagccgct cctgatacga catcgccgtc cactccagac caaagccgtt
    11281 tggcaactgc ttcactaacg attccattat atccatcgcc gtaccggtac tgacccccgg
    11341 tgcggcttcc ccaacaatct caaccgcaga ataaccgtta tagcgttcca gacgcggcga
    11401 gccagtctcc cagcgtgagg tcgcgaaagc agagaagggc accatgccgc catctttatt
    11461 tcggacatac cagagattaa tgtcatctgg cagcatgcga tacggcgcag ctgcctgcac
    11521 atagactttc ttcacacgac cgcgatccat aaagtcattc acatagctcg aaccccaggc
    11581 ggtttgcagt gtgtcgttaa tatcgtcgat agcaacgccc agcgcctgag ctttacgctg
    11641 gtcgatatca atctgcaact gcggactgtc atcgaggccg ttatggcgca cacgggttag
    11701 ctccgggttt tccgccgcca gcgccagcaa ctggttacgt gctgccatca gcgcatcgtg
    11761 acctgctcca gcgtggtcct gcaactccat atcaaaaccc gcagaactac caagaccgct
    11821 aattgctggc gggctgctgg cgataacgcg agcttcttta atcttgttaa acgcctttgt
    11881 tgcacgctcg ataatggcaa acgaggtgcc ggtcttactg tcgcgttcgc tccagtcttt
    11941 caggcggata aacattcgcg ccacgttttg cccgttaccc ccagggccag aaccaacggt
    12001 ggcaaacacc gacatgatgt tgtctttttc atgggtgaag taatatttct cgatttgctc
    12061 aacgactttc agggtctgtt gttgggttga accgctgggc aattgtaccg aggtagtaaa
    12121 catgccacgg tcttccagcg gtaagaacga cgtcggcaaa cgcaggaaca ggaacaccat
    12181 gccgccaagc agcaggacat aaatcacaat ccagcgcagg ctgcggtgga gaattttcgc
    12241 cacccctttt tcgtagcgtt cggcgttgcg gttaaacatc tggttaaacc aggcaaaaaa
    12301 gcctttttgc ccgtgatgct cacctttttt cagtggctta agtagtgtgg cacacagagc
    12361 cggagtgagg atcatcgcta ccagtactga cagcaccatc gccgcaacaa tggtaataga
    12421 gaactggcga tagatggcac cggtggtgcc gccgaagaag gccattggta caaataccgc
    12481 cgaaagcacc atcgcaatcc cgaccagtgc cccctggatc tgccccatcg atttacgtgt
    12541 ggcttcgcga ggagcgagtc cttcctcact cataatacgt tcgacgtttt ccaccaccac
    12601 gattgcgtca tccaccagca gaccgatcgc cagtaccatc gcgaacatgg ttaaggtgtt
    12661 gacgctgtaa ccgaaggcgt aaagtacgga gaaggttccc atcaacacca ccggcacggc
    12721 gatagtgggt atcagcgtgg cgcggaagtt ttgcaggaac agatacataa cgaggaaaac
    12781 cagggcgata gcttccagca gcgttttcac cacgtcttca atggaggctt taacaaacga
    12841 ggttgtttca tacgccacct tgtattccag tccgtgcggg aagtactgcg ccagctcgtc
    12901 gagacgattg agcaccagct ccctgtcgcc atttcgtttg cgccggaggc cagttttacc
    12961 cccagcccgg aggctggctt accattgaag cggctaagat aatcgtattt ctccgccccc
    13021 atttcgacgg tggcgacatc gcccagcctt acctctgagc cgtcttgatt cacccgcaag
    13081 gtgatatcgc ggaactgttc tggtgtttgc agcagtgact gggcgttaat ggtggcgttg
    13141 agcgcctgct tatcgacgga aggtgtacca ccaagttgcc caaccgcaat ctgcgcgttc
    13201 tgtgactcaa tggcatcagt gacatcttta gccgtcatct ggaaactgtt gagtttcgcc
    13261 ggggccagcc agatacgcat ggaatattgc gaaccatagg catcgatatc cccgacgcca
    13321 ttcacgcggc ttaacgggtc ctgaatatta ctggcaacat aatcggcaat atcctgttta
    13381 tccatcgaac catcggtaga gacgaaagca atggtcagaa tattggtatc gccggtttta
    13441 cgcaccgtca cgccctgatt ttgcaccgcc tgcggtaact ttcgcatggc tgattgcagc
    13501 tggttttgta cttgctgcac ggcttcgtcc ggatcggtgc ctgctttaaa acttaaagtg
    13561 acagatgcct gaccggtgcc actgctctga gatgacatat acatgagatt atcgaggccg
    13621 gtcatatttt gctcgataac ctggggcacg gtgttttcca gcgtctgggc cgatgcgccg
    13681 ggatagttag cggtcactcg cacattcggt ggcgcgagat cggggtattg ttcaacgggc
    13741 aatgaaaaaa tcgccagggt ccctgtcaga cacaacagga ttgccagcac ccaggcaaaa
    13801 atggggcgat caataaagaa attcgccatt aaaagaggac ctcgtgtttc tgcgtatcgt
    13861 tgtaggcttg acttgcttca ctgtagcggc aaggtacgag ccaatcgtgg agaaaaaagg
    13921 agttaatgta aattatcatg cccttttata ctgaacctta agtgcaagta ttctttggtc
    13981 agtaggaggc attacatcaa ctgactttcc tcaccctctg cagagcgaaa gctaatactc
    14041 actaacgtgc cgccgccgga aggttgtgaa aaagtcagcg tcccacccag ccgttccgcg
    14101 cgttcgcgca tgatattcag accataatga ccttcgggtt ctttcggttc accgatacca
    14161 atcccgttat cacggatata aaccgtgtga ttgccgtccg gcgcggtgac gcaactgacg
    14221 gcgatttcgc tggcgttggc gtgcttcatc gcattcagca ccgcttcgcg aataatttgc
    14281 aacaaatgca cctgcatttg cgcatccagc gcaagggttg gcagacggca gtcgagggtc
    14341 agtttggcgc tggtttgatt ttgtaacgta tccagcattt ccctcaacgc ggaggggaga
    14401 tccgcctgct gcagcgtcag gcggaaagta gtcaacagct cgcgtaactg ccgataagca
    14461 tcattcaacg cctgggaaaa atcggccatg atactttgtg cggtggcgtt atcttccggt
    14521 atcgaacgct tcagtaacgt caactggata cgtaagtaag aaagtacctg agccagcgag
    14581 tcgtgcaatt cgcgggcgat ggttgcacgt tcttccatca acaataaatg ctgaaaatgc
    14641 ttctgcgcct gattaaagta caaaccgcgt cccagcatcg acgaaacgct gttaagcagc
    14701 ggttctgatg atgaaacgtg actattttgc cagtgcagtt cgccgtaaac cgtctcttgc
    14761 attgtcaccg gtaaaatctg catcggcaat tccgggtttg gttgcccttc gctaatccgc
    14821 cagttttcac cgacatttaa ctccagatat tcagccgctt cattgtcgcg aacaatctgc
    14881 aaaatatggc ggaaacaatg cacatcaatc tggcttgtgt tcagcgcctg cgaacattga
    14941 tacaacacct ccagacgacg cttggcctcg tggagatcgc gggtcttttc ttctactgac
    15001 gcctccagcg aaaggtacaa tttatgcagc tcgctcgaca tctggttaaa ggtttttgca
    15061 agcagaccaa gctcattcgg cagactggta tccagcggcg gcgagtcgaa ctgcccgtgt
    15121 tcaatacgtt gactggcggt aaccagctga ttcagcgggg caaccacctg atggcgtata
    15181 cggcgcaaag taaaaaagac cagcgtgaaa ataccgatac cgccagccag ggaaatcgcc
    15241 accaccagca gcattttgcg ttcagcgtag tgctgtaaag ccagcacgaa caggtctatc
    15301 tgattaacat aattattaat attggcctga taccacggca aatcgccctt gctgagccga
    15361 ttattcatct ccagccagtt ggcattcaga tgcgcatagc gagtttttac tgcttctggc
    15421 acataccaga cgttgagatt ggttaatacc ggtgaatgca gtgcctgctg aaacaactgg
    15481 cgatgtgcat tgagttgtgg actgccactt tgcaagtcgt agcccaggcg gtaactctgc
    15541 atacgcagcg atccggcaat attgatagcc tcagcgtcgc gcaaactgct cgccagagtt
    15601 agcagagcga tacccgtgga aagaatcgac agcagcacaa tgtaaaaaaa ggcccgggcc
    15661 agactggccg agacgggtcg tttaacaatc acacgcgtct tctccacaaa aaatcattaa
    15721 tgttccagtt cgctgaaacg caatttagtc agtgatggga ttatttgagc tgccacagtt
    15781 tatagcgaaa tagttactta ttctgggcac atgaacattg ccgattcccc tgtaagtcgg
    15841 gtaataacaa cactcatata aagaataagg tttttacaac caaaaaagaa ggtcgttatg
    15901 aatcgtttta ttatggccaa cagtcagcaa tgtctgggtt gtcatgcttg tgaaatcgcc
    15961 tgtgtcatgg cttacaatga tgagcaacat gtcctgagcc aacaccattt tcatccccga
    16021 attacggtta tcaaacatca gcagcaacgt agtgcagtga cctgtcacca ttgtgaagat
    16081 gcgccctgcg cccgtagctg ccctaatggc gcaatcagcc acgttgatga cagcattcag
    16141 gtcaatcagc aaaagtgtat tggctgtaaa tcctgcgtgg tggcctgtcc gtttggtacg
    16201 atgcaaatcg tcctgacacc cgtcgcggca ggaaaagtaa aagccacggc gcataaatgc
    16261 gacctttgtg cagggcgcga aaacggtcct gcctgtgtcg agaattgccc ggcggacgcg
    16321 ctgcaactgg tcactgacgt cgcactctcc ggcatggcga aatcccgccg cttgcgcacc
    16381 gcacgtcagg agcatcaacc gtggcatgcc agtaccgctg cacaagaaat gccggtaatg
    16441 agtaaagtcg aacaaatgca ggcaacgccc gcgcgtggcg agccggataa actggcgatt
    16501 gaagcgcgca aaaccggttt tgatgaaatt tatctgccat ttcgcgccga ccaggcacaa
    16561 cgggaagcct cgcgctgcct taagtgcggc gagcacagcg tttgtgaatg gacctgcccg
    16621 ctgcataacc atattccgca gtggattgaa ctggtgaaag ccggaaacat cgacgccgcc
    16681 gtcgagcttt ctcaccagac caacaccctg ccggaaatta ccggacgcgt ttgtccgcaa
    16741 gaccgtttgt gtgaaggtgc ctgtactatt cgcgatgagc acggcgcggt aactatcggc
    16801 aacattgaac gctatatttc agatcaggcg ttggcgaaag gttggcgtcc tgacttaagt
    16861 catgtcacca aagtggacaa gcgggtggcg attatcgggg caggtccggc agggctggcc
    16921 tgtgcggatg ttctgacccg caatggcgtg gcggtgacgg tgtacgatcg ccatccagaa
    16981 atcggtggct tgctcacttt cggcattcct tctttcaaac tggataaatc cctgctggca
    17041 cgccgtcggg aaatcttcag cgcgatgggg attcacttcg aactcaattg tgaagtgggt
    17101 aaagatgtct ctttggattc gcttttggaa caatacgacg cggtcttcgt tggcgtaggc
    17161 acttaccgtt ccatgaaagc gggtttaccc aatgaagatg cgccgggcgt ttatgacgcg
    17221 ctgccgttcc tcatcgccaa cactaaacag gtgatggggc tcgaagagct accggaagag
    17281 ccgtttatca ataccgccgg acttaacgtc gtggtactgg gcggcggcga caccgcgatg
    17341 gactgtgtgc gtactgcgct gcgccacggc gcgagtaacg tcacctgcgc ttatcgtcgt
    17401 gatgaagcta acatgccagg ctcgaagaaa gaggtgaaga acgcccgcga agagggggcc
    17461 aacttcgaat ttaacgtcca gccggtggcg cttgagctga atgaacaagg tcacgtttgc
    17521 gggattcgtt tcctgcgcac gcgtcttggc gagccggatg cctaggggcg tcggcgtcca
    17581 gtaccggtgg aaggcagaga atttgtcatg ccagccgacg cggtgattat ggcgtttggc
    17641 ttcaatccgc acgggatgcc gtggctggag tcgcacggtg taacggtaga caaatggggc
    17701 cgcatcatcg cggatgtgga aagccagtac cgttaccaga ccaccaatcc gaaaatcttc
    17761 gccggtggcg acgccgtgcg tggtgcggat ctggtggtta ccgcaatggc agaaggacgt
    17821 catgcggcac aggggattat tgactggctg ggggtaaaat cagtcaaatc tcactgatag
    17881 cctgcgcaga caaacccgac ttcacagcgt aagataattg ttcatttcgc gctgtggagt
    17941 cggtatgacg caacaaatca ccctcattaa agacaaaatt ctctccgata actatttcac
    18001 cctgcacaac attacctacg atctcacgcg caaagatggc gaagttatcc gccataaacg
    18061 tgaagtttac gatcgcggta atggtgcgac gatcctcctg tacaacgcga agaaaaagac
    18121 cgtggttctg attcgtcagt tccgtgtcgc tacctgggtt aatggcaatg aaagcgggca
    18181 gctgattgaa acctgcgccg ggctgctgga taacgacgaa ccggaagtgt gcattcgtaa
    18241 agaagcgatt gaagagacgg gctatgaagt gggcgaagtg cgcaaattat ttgaactgta
    18301 tatgtcgcca ggcggtgtga ctgagctaat ccactttttt atcgccgaat acagtgacaa
    18361 tcagcgcgct aacgccggtg gcggtgtcga agatgaagat attgaagtgc tcgagctgcc
    18421 gttcagccag gcgctggaga tgatcaaaac cggcgagata cgtgacggta agacggtgtt
    18481 attgcttaac tatttgcaaa cgtcacattt aatggactga aaaataacaa taatatttcg
    18541 ttgtttatta ttggctcaat ccgacaaatc cgattgagcc gcgctacctg cgcaacgaag
    18601 atacgacttg tgctgtttgt ttgaacttct ggggtcgtac cgtccatgcg ctatcgcatt
    18661 ttccttctct tttttttcgc tttgttgccg acgtctttgg tgtgggcggc accagcgcaa
    18721 cgggcgtttt ccgactggca ggtcacctgt aataaccaaa acttctgcgt ggcgcgtaat
    18781 acgggcgatc ataatggact ggtgatgacc ctgagccgca gcgccggggc gcataccgat
    18841 gccgttttac gtattgagcg cggcggattg aagtcgccgg aggcgtcaga aggggagata
    18901 gcgccacggc tgctgttaga tggcgagccg ttggcgttaa gtggtgacaa gtggcggatt
    18961 tcaccatggt tattagtaac cgatgatacg gcaaccatca ccgcgttttt gcagatgatt
    19021 caggaaggga aggcgatcac cttacgcgac ggcaatcaga ccatttcgct gagtggctta
    19081 aaagcggcgt tgttgtttat tgatgctcag caaaagcgcg ttggcagtga aaccgcgtgg
    19141 atcaagaaag gggacgaacc gccgctcagc gtaccgcccg cgcctgcgct gaaagaggtc
    19201 gcggtggtta acccaacgcc gacgccactc tcactcgaag aacgcaacga tttgctggat
    19261 tatggaaact ggcggatgaa tggtctgcgc tgctcgcttg atccattgcg tcgtgaggtg
    19321 aatgtcactg cgctgactga tgataaagcg ctgatgatga ttagctgtga ggcaggggcc
    19381 tataacacca ttgatttggc atggattgtg tcgcgtaaaa atccgctggc ctcgcgcccg
    19441 gttcggttgc gtttgccgtt caacaacggg caggagacga atgaactgga actgatgaac
    19501 gcaacatttg atgagaaatc gcgtgaactg gtgaccttag cgaaagggcg cggattaagc
    19561 gattgtggca ttcaggcgcg ctggcgcttt gacggtcagc gttttcgcct ggtgcgttac
    19621 gccgcagaac ccacctgcga taactggcat gggccagacg cctggcccac gttgtggatc
    19681 acccgttaaa caaaatggcg gggacatgta ggccggataa gacgctgtag gccggataag
    19741 acgcgtcaga gtcgcatccg gcctacagcg tccggcaatc agcatccggc gatcaccatc
    19801 aggcaccttt cactcccagc accttatgcg cttttgccac aatattctcg gcggtaaagc
    19861 caaagaacgg gaacagctta tccgccggag cagattcccc ataacctgtc atcccaacaa
    19921 tcgccccttt caaaccaaca tacttgtacc agtaatcggc aatacctgct tccaccgcca
    19981 cgcgagccgc aacgttagaa ggcaacaccg actcccgata ttcctcatcc tgggcgtcga
    20041 aaatatcagt cgagggcagg gaaactacgc gtacattgcg accttctccc gctaatttct
    20101 ctgccgcttg cagggggatt tccatctctg aaccggtggc aatcaaaata atatctggct
    20161 taccgccgct gtctttcagc acataaccgc cacgggcaat ctcttttacc tgatccggcg
    20221 tacgttccac ttgggccaga ttctgccttg agaggatcag tgccgtcggt ccgttgtggc
    20281 gctcaaccgc cagcttccag cccaccgccg cttccacctg atcgcacggt cgccaggtgc
    20341 tgaaatttgg cgttaagcgc aggctggcca gttgctcaac agcctggtgc gtcggaccat
    20401 cttcgcccag gccaattgag tcgtgggtat aaaccataat ctgccgcgct ttcatcagtg
    20461 ccgccatccg cgcggcgtta cgggcgtatt caacaaacat caggaacgtg gcggtatacg
    20521 gcacaaagcc gccgtggtgc gcgatgccgt tggctatagc ggtcatgcca aattcacgca
    20581 ccccgtagtg aatgtagttg cccgccggat cttccttcag cgaaacagaa cctttccaga
    20641 tggtcaggtt gctggagcca gatccgccga accgccgagc aactcaggca gcatcggc
//
LOCUS       DAJTMC010000064        20655 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.064, whole genome shotgun sequence.
ACCESSION   DAJTMC010000064 DAJTMC010000000
VERSION     DAJTMC010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20655
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.064"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            <1..876
                     /locus_tag="OO130_002059"
     CDS             <1..876
                     /locus_tag="OO130_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CS1-pili formation C-terminal domain-containing
                     protein"
                     /protein_id="HCR5365480.1"
                     /translation="DQTSWQLSHTFYNDSFSHKELGVTVGGLNTDTINSAVNGRWDGQ
                     YGNVYATVSDSYDRKNHDHLSAFTGTYSSTLAVSRYGVNLGASGTDDLLGAVLVDVKG
                     FSEQDEESQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGFVEVNDSSQGNQQGTTN
                     IINGAGNRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKKFPIVGLNSAMLLVAED
                     GGFTLEINGSEKELYLLSGQQFLKCPLSVEKKRASIRYSGDVTCSVVTYSQLPELKQP
                     KLRGNVQTAQREVAP"
     gene            873..1962
                     /locus_tag="OO130_002060"
                     /pseudo
     CDS             873..1962
                     /locus_tag="OO130_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CblD"
     gene            complement(2050..3348)
                     /gene="exuT"
                     /locus_tag="OO130_002061"
     CDS             complement(2050..3348)
                     /gene="exuT"
                     /locus_tag="OO130_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HCR5365481.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSRGGLA
                     IARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFSLVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQESHNDPAPQH"
     gene            3831..5243
                     /gene="uxaC"
                     /locus_tag="OO130_002062"
     CDS             3831..5243
                     /gene="uxaC"
                     /locus_tag="OO130_002062"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HCR5365482.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            5258..6745
                     /gene="uxaA"
                     /locus_tag="OO130_002063"
                     /pseudo
     CDS             5258..6745
                     /gene="uxaA"
                     /locus_tag="OO130_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
     gene            6828..7379
                     /locus_tag="OO130_002064"
     CDS             6828..7379
                     /locus_tag="OO130_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HCR5365483.1"
                     /translation="MTAYWLAQGVGFIAFLIGITTFFNRDDRRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTRALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAVDERG"
     gene            complement(7384..8628)
                     /gene="sstT"
                     /locus_tag="OO130_002065"
     CDS             complement(7384..8628)
                     /gene="sstT"
                     /locus_tag="OO130_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HCR5365484.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDLSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(9244..10209)
                     /gene="alx"
                     /locus_tag="OO130_002066"
     CDS             complement(9244..10209)
                     /gene="alx"
                     /locus_tag="OO130_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HCR5365485.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINARVNYRHDKQRGE"
     gene            complement(10491..11477)
                     /locus_tag="OO130_002067"
     CDS             complement(10491..11477)
                     /locus_tag="OO130_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR5365486.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNALHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTASLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLLHSKVSDSVLESEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL"
     gene            complement(11556..12239)
                     /locus_tag="OO130_002068"
     CDS             complement(11556..12239)
                     /locus_tag="OO130_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HCR5365487.1"
                     /translation="MQPRLLLRICFSRRTLKIACLLLLVAGATIFIADRVMVNASKQL
                     TWSDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKNYDE
                     ASGMQKALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRAIWL
                     AKQYSIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHSQPKYLGPSVMIGPF
                     SEHGCPAKE"
     gene            complement(12316..12819)
                     /locus_tag="OO130_002069"
     CDS             complement(12316..12819)
                     /locus_tag="OO130_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HCR5365488.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            12904..14040
                     /gene="rlmG"
                     /locus_tag="OO130_002070"
     CDS             12904..14040
                     /gene="rlmG"
                     /locus_tag="OO130_002070"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HCR5365489.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            14325..14639
                     /gene="higB"
                     /locus_tag="OO130_002071"
     CDS             14325..14639
                     /gene="higB"
                     /locus_tag="OO130_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HCR5365490.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            14636..15052
                     /gene="higA"
                     /locus_tag="OO130_002072"
     CDS             14636..15052
                     /gene="higA"
                     /locus_tag="OO130_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HCR5365491.1"
                     /translation="MIAIADILQAGEKLTAVVPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLADLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(15097..17115)
                     /gene="fadH"
                     /locus_tag="OO130_002073"
     CDS             complement(15097..17115)
                     /gene="fadH"
                     /locus_tag="OO130_002073"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HCR5365492.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFAHCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLHTNQRSDQWGGDYRNRMRFAVEVVRAVRERVGNDFIIIYRLSM
                     LDLVEDGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSESGGQFNIAKQIPGKEEFYETLRYYHRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELNARRAIAQGTRLALEI"
     gene            complement(17541..19892)
                     /gene="ygjK"
                     /locus_tag="OO130_002074"
                     /pseudo
     CDS             complement(17541..19892)
                     /gene="ygjK"
                     /locus_tag="OO130_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417551.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
     gene            complement(19909..20655)
                     /gene="ygjJ"
                     /locus_tag="OO130_002075"
                     /pseudo
     CDS             complement(19909..20655)
                     /gene="ygjJ"
                     /locus_tag="OO130_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417550.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
BASE COUNT         5034 a         5415 c         5429 g         4777 t
ORIGIN      
        1 gatcaaacgt catggcagtt atcgcatacc ttttataatg attcattcag ccataaagaa
       61 cttggcgtaa ccgtcggagg cctgaacacc gataccataa acagcgcggt taacgggcgt
      121 tgggatgggc aatacggaaa tgtctacgct accgtatctg atagttatga ccgtaagaat
      181 catgatcatc tttcggcctt tacgggcact tatagctcta cgctggctgt cagtcgctat
      241 ggcgttaatt tgggagccag tggtacagac gatttgctgg gtgcggtatt ggtggatgtg
      301 aaaggcttct ctgaacagga tgaagagagt caggatctgc aactcgaagc gcgggtggca
      361 ggcagccgaa ctttgcagct tggtcaaagc gacagtgtgt tgttccctta tcctggattt
      421 cagtctggtt ttgttgaggt taacgacagt agccagggca atcagcaggg gacaacaaac
      481 atcattaacg gtgcagggaa tcgtgaatta atgttgttgc ctggcaagct gcgctatcgc
      541 gaagtgtctg ccagctttaa ctacaactat atcggtcgtt tattactgcc ggcagcggtg
      601 aaaaaattcc cgattgtggg gctgaacagc gccatgttac tggtagctga agatggcgga
      661 tttacacttg aaattaacgg cagcgaaaaa gagctctatc tgctttccgg gcagcaattc
      721 cttaagtgtc cactgagtgt tgaaaagaaa cgcgccagca ttcgttacag cggagatgtt
      781 acttgtagtg tggtgactta ttcacaatta ccggagttga aacagcctaa attacgtgga
      841 aacgttcaga cggcgcaaag ggaggttgca ccatgagaaa tcgattgatt gcggcgatat
      901 tgggcttgtt tggcacgctc actggcgttc aggcagctcc tgacgtgacc agtgaaatta
      961 cgtatgattt ggcatctggt agagcggatt attacttctg gaaggatgaa gcgtcggcag
     1021 gaaataatgg atatatgtgg tatgaatgtt cgtatcctga cctccaacaa acctgtacag
     1081 ctaatggaaa tatatcgaca gtacaaatct atttaactga acaacgcagt gggatgcgtt
     1141 ggccggtaaa actcaaagga tttaaaacag ccattgtaag tagcgatgaa gcgccgctag
     1201 gatgcaaggg ggggcaaagg gcttcagacg aatcttaagg attctaatag atcttcatgt
     1261 acagaagatg gtcaacatta ttatatatac gatacaaagt ttcttacgct ctaccttgag
     1321 cagacagaga tgaagaattt gccgattggt ggcgtctgga aggggaaagt taaattacat
     1381 tcgaacagcc cggcccagga ctatttcgca aatattaccc tgaatacgct cgaccccaac
     1441 catattgacg tgttcttccc ggagttcgcc cacgccacgc cgagggtgca gttagacttg
     1501 catccaacag gaagcgttaa cggcagcaac tacgcgcaag atctgaccat gttggacatg
     1561 tgcctgtacg atggttttaa cggtaatgcc atcagttatg aaatcatgct caaagatgaa
     1621 gggcgaccag ctgcagggcg cagagacggt gacttctcta tctatcgtca gggaggaacc
     1681 tctaccgacg agggagaacg cattgattac cgggtcaaaa tgtacaaccc ggaaaccggt
     1741 gggcaaattg atgtgcgcaa taatgaaaat atggtctgga acagcattaa cctgaaaagt
     1801 gtgcgtccgg tggtactgcc cggtattcgc tatgccgtga tgtgtgtgcc gacacccttg
     1861 gctctggcag tcgataaatt cagcgtgatg gataaacagg ccgggtacta catgggcaaa
     1921 ttgtcagtaa tctttacgcc ttccttgcca accatcaatt aaccctgcaa agcggacaaa
     1981 cgcttttttc tatataaacg acgaagccgc ctgataaggc ggcttcgtcg ttataactta
     2041 cgttaaacgt taatgttgcg gtgcgggatc gttatgtgat tcctgcgcta cctcaatcgc
     2101 cggtttgttc tgcaacacgg tccagataac cagcgcacct aacaggtcga acactgccag
     2161 tactgcgaac agcgggctga agccgatggt gtcagccagt gcaccgacaa ccagcgaaaa
     2221 cagcgtactt gccagccatg cggacatccc ggttaaaccg ttcgccgtcg ccacttcgtt
     2281 acgaccgaac acgtcagaag agagcgtaat cagcgcgcca gacagtgcct ggtgagcaaa
     2341 accaccgata cacagcagca taattgcgac atacgggttg gtgaacaggc cgatcatacc
     2401 cgggccaatc atcagcactg cacccagcgt tacgaccatc ttacgggaaa cgatcaggtt
     2461 cacaccaaac caacgctgga acagcggcgg caggtaacca ccgaggatac aaccgaggtc
     2521 agcaaacagc atcggcatcc aggcgaacat cgcgatctct ttcaggttaa agccgtaaac
     2581 tttaaacatg aacagcggga tccacgcgtt aaaagtaccc caggctggtt ctgccaggaa
     2641 acgcggcagc gcgatacccc agaactgacg gttacgcagg atctgaccaa cggacatttt
     2701 cttcgccgtg ctcacctggt gctgggcttc ctgaccatta ataatatagt cgcgttcttc
     2761 atcggtcaga tgcttctggt cgcgcggatg tttatagaaa atcagccatg ccatcgccca
     2821 gataaagctc aatgcaccgg agatgataaa tgccatctgc cagctgtgca ttacgattgc
     2881 ccataccacc agcggcggcg caatcatcgc accaatcgaa gaacctacgt taaagtaacc
     2941 taccgcgatg gaacgctctt tcgccgggaa ccattcggag ctggctttca gacccgccgg
     3001 gatcatcgct gcttccgcgg caccgactgc accacgagca atcgccaggc cacccctgct
     3061 accagccagc gcggttgcac cacagaatac ggcccacagt acagcaaaca ttgcataacc
     3121 gattttcgta cccagcacat ccagtacata gcccgctacc ggttgcatga ccgtataagc
     3181 agcagaatag gctgcgatga tataggaata ctgttgggtg gagatgttta actcttccat
     3241 cagagttggc gcagctgccg ccacagtgtt acgcgtcagg taaccaagca cggtgccaag
     3301 cgtcaccagt gcgatcatat accaacgtaa ccctttaatt ttacgcatgt aaaacctcat
     3361 cgttttgtta tatccgcagg aagcgcggcc atcatcccgc cagaggctga agatgttagt
     3421 tacgaccggg taaccgaaaa aaaaccggca cgccccgaac gttgccatga aagtttcgtg
     3481 ctaattcggc ttccgtaccg gttaacccga ttcatctaac aaatgaatga atcggcaatt
     3541 tgcattccgt cgacttatga tttgcgacgg cagaaagatg acttgtcata caactttaaa
     3601 aggtgagagc catcataaat gtgcgaatat ttgtagggac attacctgac gacagcgagg
     3661 ccagtactgg cgcggcctgc agcgagattt accactttga gagtaatttt tttaactacg
     3721 tttattgatc taactcacga aaatatcttc ggactctgga aattggtgtg ataactttgt
     3781 cagcatcgca ccataagcaa gctagctcac tcgttgagag gaagacgaaa atgactccgt
     3841 ttatgactga agatttcctg ttagataccg aatttgcccg ccgtctgtat cacgactacg
     3901 caaaagacca gccgattttc gattaccatt gccatttgcc gccgcagcag attgcggaag
     3961 actatcgttt taaaaacctg tatgacatct ggctgaaagg cgatcactac aaatggcgcg
     4021 ctatgcgtac caacggtgtg gccgagcgtc tgtgtaccgg tgatgcgtct gaccgtgaaa
     4081 aatttgacgc ctgggcggcg actgttccgc acactatcgg caacccgtta taccactgga
     4141 cgcacctcga actgcgtcgt ccgtttggta tcactggcaa attgctttct ccgtcaactg
     4201 ccgatgaaat ctggaacgaa tgtaacgaac tgctggcgca ggataacttc tccgcgcgcg
     4261 gcatcatgca gcagatgaac gtgaaaatgg tcggcaccac cgatgacccg atcgattctc
     4321 tggagcatca cgcagagatc gccaaagacg gctctttcac catcaaagtg ctgccgagct
     4381 ggcgtccgga caaagccttc aacatcgaac aggcgacctt taacgactac atggcgaagc
     4441 tgggcgaagt ttccgatacc gacattcgcc gctttgctga cctgcaaact gccttgacca
     4501 aacgtctgga tcacttcgcc gctcacggct gtaaagtgtc tgaccatgcg ctggacgtgg
     4561 tgatgtttgc tgaagcgaac gaagcggaac tggacagcat tctggcgcgc cgtctggctg
     4621 gcgaaaccct gagcgagcac gaagtggcac agttcaaaac tgcggtactg gtgttccttg
     4681 gtgccgaata tgcacgtcgc ggctgggtac agcagtacca cattggcgcg ctgcgtaata
     4741 acaacctgcg tcagttcaaa ctgctggggc cggatgtagg ctttgactcc atcaacgacc
     4801 gtccgatggc agaagagttg tctaagctgc tgagcaagca gaacgaagaa aacctgctgc
     4861 cgaaaaccat tctgtactgc ctgaacccgc gcgataacga agtgttgggc accatgatcg
     4921 gtaacttcca gggcgaaggt atgccgggca aaatgcagtt cggttccggc tggtggttta
     4981 acgatcagaa agacggtatg gaacgtcaga tgacccaact ggcgcagctc ggtttgctga
     5041 gccgctttgt cggtatgctg actgacagcc gtagcttcct gtcatacacc cgtcacgaat
     5101 acttccgccg cattctgtgc cagatgatcg gtcgctgggt ggaagcgggc gaagcaccgg
     5161 cggacatcaa cctgctgggc gagatggtga aaaatatttg ctttaacaat gcgcgtgact
     5221 acttcgccat tgaactgaac taaggtctgg ggttgatatg caatacatca agatccatgc
     5281 gctggataac gtcgcggtcg ctttagcgga tttggctgaa ggcacagaag tcagtgtcga
     5341 taaccagact gttacgctgc gccaggatgt tgctcgtgga cataaatttg cgttaacgga
     5401 tatcgcaaaa ggggccaatg tcatcaaata tggcctgccg attggttatg cattggcgga
     5461 tattgcggcg ggggaacacg tccacgccca caatacgcgc acgaatctga gcgatctgga
     5521 tcagtatcgc tatcaacctg attttcagga tctgcctgcg caagcggcag atcctgaagt
     5581 gcagatctat cgtcgcgcta acggcgatgt cggggtgcgt aacgagctgt ggatcctgcc
     5641 aaccgtgggc tgtgtcaacg gcatcgcgcg gcagatccag aaccgttttc tgaaagagac
     5701 caacaacgct gaaggtaccg acggcgtgtt cctcttcagc cacacctacg gctgctcaca
     5761 gctgggcgac gatcacatta atacccgcac catgctgcaa aacatggtgc gccacccgaa
     5821 cgcaggcgca gtgctggtga ttggtctggg ctgtgaaaac aactaggttg ccgcattccg
     5881 tgaaacgctg ggcgatatcg atcctgaacg cgttcatttc atgatctgcc aacagcagga
     5941 tgatgagatc gaagccggga tcgagcattt gcatcagctg tataacgtga tgcgcaacga
     6001 taaacgcgag ccaggcaaac tcagcgaact gaagtttggt ctggagtgcg gtggttctga
     6061 cggtctttct ggtattactg ctaacccgat gctggggcgt ttctctgact acgtgattgc
     6121 taacggcggt actaccgtac tgaccgaagt gccggagatg tttggcgcag agcagttgct
     6181 gatggaccat tgccgcgacg aagcaacgtt tgaaaaactg gtcaccatgg tcaacgactt
     6241 taaacagtac tttattgccc atgatcagcc gatctacgaa aacccatcac cggggaacaa
     6301 agcgggcggt atcaccacgc tggaagacaa atcacttggc tgtacccaga aagcgggttc
     6361 cagtgtcgtg gttgacgtgc tgcgttacgg cgagcgtctg aaaacgccag ggctgaactt
     6421 gttaagtgcg ccgggtaacg atgccgtagc gaccagcgcc ctggcgggtg cgggctgcca
     6481 tatggtgctg ttcagtactg gtcgcggcac gccatacggt ggatttgtgc cgacggtgaa
     6541 aatcgccacc aacagtgaac tggcggcgaa gaaaaaacac tggctcgact ttgacgcggg
     6601 tcagctgatc cacggtaaag cgatgccgca gttgctggaa gaatttatcg acaccatcgt
     6661 tgagtttgcc aacggtaaac aaacctgcaa cgagcgtaac gacttccgcg aactggcgat
     6721 cttcaaaagc ggcgtaacgc tataaagtcg aaatgagttg ttaataaaaa acggcgtttc
     6781 ataatgtgaa gcgccgtttg cttttccata cctgataagg atttatcatg accgcgtatt
     6841 ggctggccca gggcgtgggt ttcatcgcct ttctgattgg tatcacaaca tttttcaatc
     6901 gtgacgaccg tcgcttcaaa aagcagcttt cggtctatag cgccgttatt ggcgtacatt
     6961 tttttcttct gggcacctat cccgctggtg ccagtgccat ccttaatgcc attcgtacat
     7021 tgattacctt acgcacgcgc agcttatggg taatggcgat ttttattgtg ctgactggcg
     7081 gaattggcct cgcgaagttc catcatcctg tcgaactatt gccggttatc ggtacgattg
     7141 tcagtaccag ggcgctgttc cgctgtaaag ggctcaccat gcgctgcgtg atgtggtttt
     7201 caacgtgttg ctgggtgatt cacaacttct gggcggggtc gataggcggc acaatgattg
     7261 agggtagttt tctgcttatg aatggcttga atatcattcg tttctggcgg atgcagaaaa
     7321 ggggaattga tccgtttaaa gtagagaaaa cccctcccgc cgtagacgaa aggggttaaa
     7381 caattaatta cgcagggcgc tatttgccag acgatcgtct tccgcctggc aagctgccgc
     7441 agtgaacagc acgtcagttg aagagttcag cgcggtttcg caagagtcct gcaatacgcc
     7501 gatgataaag ccgacggcaa ccacctgcat ggcgatatcg ttcgaaatac cgaacatatt
     7561 acaggccagc gggatcagca gcagagaacc ccccgccacg ccggatgcgc cacaggcaca
     7621 cagagaagcc accacgctca acagcagcgc cgtgggcaga tcgaccggga tacccagcgt
     7681 attaaccgca gccagcgtca acacggtaat ggtgattgct gcgcccgcca tattgatggt
     7741 ggcacccagc ggaatagaaa cggaataggt atcgcgatcc agattcagct tttcacacag
     7801 tgccatattc accggaatgt tcgccgcaga gctgcgggtg aagaaggcat acacgccgct
     7861 ttcgcgcagg cacagcagca ccagcgggaa cgggttacga cgaattttcc accacaccag
     7921 caatgggtta accaccagcg ccaccagtaa catacagcca accagcacga ccagcagttg
     7981 cgcgtagccc cacagtgtgg agaaaccggt ggttgccagg gtagaagaaa ccagaccaaa
     8041 aataccaatc ggtgcgaagc gaatgaccag tttcaccata aaggtgacgg cattcgacag
     8101 atcgttgacc aggtttttgg tggtctcgtt accgtgacgc agtgcgaaac cgaggccaat
     8161 tgcccacacc aggatcccga tgtagttacc tttcagcagc gcgtcgatgg ggttggaaac
     8221 catgctcatt accagcccgc gcatcacttc tacaatgcct gacggtggcg aaatatcacc
     8281 cgcgctactg gataagtgca gggtagaagg gaaggcgaag ctgaaaacga ctgcggccag
     8341 agcagcagag aaggtaccca gcagatagag gaacaaaata gggcggatat tggttttctg
     8401 cccgtgctgg tggttagcaa tagatgccat caccagcatc aacaccagga tgggggcaac
     8461 ggctttcagt gcgccgacga acaaagtacc taacagacca acagcttccg ccgcgggttt
     8521 tgagatccat gccagaagaa tccccagaac aaggccgacc aggatttgtt ttaccaggct
     8581 gccatgagcc agacgccgga atagccccgg tgaacgttgc gtagtcattt ttcgatcctt
     8641 tcattgtgtt gtcgcacatc cctggtgcgt tctgtcgacg catctttcag gatgtaacaa
     8701 agtgtttgca aggtcgagta taaggaaagt tacagcttcg ggaagcgaaa aatgctggat
     8761 tttaaagaga ggtcatattt tgcaacttaa ttgttacatt tgtttgacat aaacagaggg
     8821 aaggactggc tactaaggct gattagataa catatgggca aaactatttg gaatatgaga
     8881 acggttgcgc tcctttaatg tgctatgcca tataaagtca ctggcgcagg gagcgcgcag
     8941 ggggcagcca atcgccgccg ccccctgcac ccccaggctc tggcgaacaa aatcgccgct
     9001 gcgcgattcc ctcagcttat cccttgcggc taccgggtcg ggcgcaaggg aacgtccctg
     9061 taagatgcgc cctcgccgcg catccatgcg cgtcgccccg gcctccaggg aacgcttcgg
     9121 cgattttgac gccaccaaca cccgtgcggt ctttttgtaa agagctacaa gccaattaca
     9181 taattaatag ctcacctttg tctgggatga gttatctcta ggaatggctt aatatacagc
     9241 gaatcattct ccccgctgct tatcatgccg ataattcacc ctggcgttga taataaacgt
     9301 catcaccaga atgccaaaca ccacgcccag cgagacggcg attggaatat ggtagaagtc
     9361 gacaatcagc atcttgatac cgataaacac caggatcacc gccaggccat atttgagcat
     9421 cgagaaacgc tctgctacgc ccgccagcag gaaatacatc gcacgcaggc cgaggatcgc
     9481 aaacaggttt gaggtcagca caatgaacgg gtcagtggtc acggcgaaga tagccggaat
     9541 gctatccacg gcgaaaatca cgtcgctcaa ttccaccaga atcagtacca gcatcagcgg
     9601 tgtggcgtac agcaatccat tcttacgcac aaagaaatgc tcgttgtcga tggtgtcggt
     9661 catgcgcaaa tgaccgcgta gccagcgcac cagcggcttg tcgccaatgc ctgattcatc
     9721 ttcatgggcg agcgccatct tcacgccggt aaacagcagg aaggcaccga agatatacag
     9781 gatccagtcg aactgcgaaa ttagccagct gccagtgaag atcatgatgg tacgcagaac
     9841 aatcgccccg agcacgccat acaccagcac gcggcgttgt aatgccgccg gaacagagaa
     9901 atagctgaac aacatcagcc agacaaagac gttatcgacc gccagcgatt tctcaatcag
     9961 ataacccgtg agaaaggcca gtgcctgtgg atcggcaacg gcgcgacctt cggtttgcac
    10021 cagataccac cagaaagcgg cgttaaacag taacgacagc gtcacccaaa ccagcgacca
    10081 ggccgcagcc tgtttcatgg tcatggcatg tgccccacga cgcccctgca acaacaggtc
    10141 gatagccagc ataatggcga caacaacagc gaatccgccc catagcaacg gcgtgccgac
    10201 agtattcata gaagttcctt acacataaaa aaaacggcca acgtcggaag acgtcagccg
    10261 ctgcttttta tgcatagacc tcgccttccg gcaaggtctc acttacaaca aaaaagaata
    10321 cgtctgcgta ttcctttcgg gttgcccggt gaccggatgt ggtttttcac acatcgtaat
    10381 gacgatcgac cggcaatgaa gttactcccc tttgcaggta acaaagtatg acagccattc
    10441 agccagtcaa tggtctgtta catacctttt acactgtttt actttggcaa ttatagtttt
    10501 acgctatctg ccggaaaaat caccccagtc tggcggcgga tctcagtcag cagtttggcg
    10561 gtgatgcgac tgaccgccag ccctggatga tccaccagat gctcatccac cagggtagca
    10621 aacagctctg cttcgtagag catggtatta atatgctgcg gctgggtgag atcctgcatc
    10681 tggctgccac gcggcacgaa gcacactttc tggcattcag acagtttttc aatcaccagc
    10741 gatcccgctt cgccctgaat ctcgctttcc aggacagaat cactgacctt tgagtgcagc
    10801 aacgtaacgc tgaaatcacc gtaatccatc accaccacgc cgtgggcatc tacgccgctt
    10861 gccaacaaac tggcggttgc ctgcacgctt ttcggttcac cgaataatgc caccgccgac
    10921 gccaggcagt aaaagccgat atccataatt gaaccgttag agaatgccgg attaaaggtg
    10981 ttgggattct caccatccag gtaacgttga taccgcgagg aatactggca atagttgaaa
    11041 aagactttac gcaatttgcc gactttcggc agcgcctggc gcagcaaatg aaagttcggc
    11101 aggctggcgg ttttaaatgc ctcaaacagc accacctgat tttcccgcgc acaggcaatg
    11161 gcggcatcca cttccgccag attcgacgcc agtggtttct cgcaaatcac atgaatttta
    11221 tggctaagga aaagttgtgt ctgggaaaag tgcagggcat tcgggctggc aatatacacc
    11281 gcgtcaatgg catcgctttc cgccatcgct tccagcgagg taaacagatg ctcgacagaa
    11341 aaatcattgg cgaagtgctg ggcctgttca aggctgcggg aatatacggc ggttaacttg
    11401 tatttaccgc tctcatgggc ggcctcgacg aactggcgag tgatccagtt cgtaccaatc
    11461 acagcgaaac gtatcataac gcgtggatac tccataaagg gattctgtcg tcaatgtagc
    11521 acgccttttt catcttcgac gtgcgcagat acgcattatt cttttgccgg acagccatgc
    11581 tcactaaacg gcccaatcat gaccgatggc ccgagatatt tcggctggct atggaggatt
    11641 ttggcgtcaa tcacggcgct gacacgagcg agtttctctc gcagttgagt ataaaaaccg
    11701 tgcttcatat tgaggtcagg cgcgttaaaa ccgatagcgt cgatgctgta ctgttttgcc
    11761 agccagatgg cgcgttggtt atgaaattct tgcgagataa tggtgatatg gttttcacca
    11821 aaaacctttt tggcacgcac taccgaatcc agcgttgaga atccggcata gtcgcagaag
    11881 atcactttcg ccggtacgcc tttagcgatc aaagccttct gcatacctga tgcttcatcg
    11941 taatttttac gtccgttatc gccacttacc agtagccatt tcacttttcc ggcgtggtac
    12001 aacgctgctg cggtatcaat acgtcgggta aagtagcgat tacccggcct ggcccccagc
    12061 aataagccca cgttacgcgc cggaactgcg ttgacatcgc tccaggtcag ctgcttgctg
    12121 gcgttgacca ttactcgatc ggcgataaaa atggtcgctc ctgccacaag taacagtaag
    12181 caggcaatct ttagagtccg gcgagaaaaa caaatacgga gaagaaggcg gggctgcatg
    12241 gtcgttatcc ttaacgaaag gggggaggtc gattttaggc gctaaagaaa gaaaagataa
    12301 cgcctgacat cattttcaga ttttgtcctg cccaagcgac aactgcgtta gccacagccg
    12361 ggtgtcgaac tccagctggt ggtactgcgg ctccatatgg cagcacaact ggtaaaacgc
    12421 tttgttatgc tctttctcct tcaggtgcgc cagttcgtgc accacgatca tacgtaaaaa
    12481 cggttccggc gcgttgcgaa acacagtagc gacgcggatc tctgcttttg ctttcagctt
    12541 gccgccctgc acacgagaaa cagcggtatg taacccgagc gcgtttttta gcacatggat
    12601 cttattgtca tacatcactt tattgatcgg cggggcattg cgcagaaact gattttttag
    12661 atcctgagta tattgccaga gggctttatc ggtggcgtag tcgtgtgttc ccggatagcg
    12721 ttttgccagc acatcgccca gacgctgttc gttaatcagc gttcgcacct gggaaagtag
    12781 ctgctcggga taaccctgaa gataagtaag attgctcata accgcccacg aaaaagagaa
    12841 aaagggtata ctcacgcacc cttttcaggg gcaacgtcga aattttatca ttcaggaggg
    12901 ccgatgagcc acttagacaa cggtttccgt tcactgacac tacaacgttt tccggcgacg
    12961 gatgacgtta acccgctaca ggcgtgggaa gcggcggatg aatatttgct gcaacagttg
    13021 gacgacacag aaatccgcgg cccggtgttg atcctgaatg atgcctttgg tgcgttaagc
    13081 tgcgcgctgg cggaacataa gccgtacagc attggcgact catacatcag tgaactggcg
    13141 acgcgcgaga atttacgcct caacgggatt gatgagtcga gcgtgaagtt tctcgacagc
    13201 accgccgact acccgcaaca gccgggtgtg gtgctgatca aagtgccgaa aacactggca
    13261 ttgctggaac aacaactgcg tgcgctgcgc aaagtggtca cgtcggatac acgtattatt
    13321 gccggtgcca aagcccgtga cattcacact tccacgctgg aactgttcga aaaagtgctc
    13381 ggcccgacca ccaccacgct ggcatggaaa aaagcgcgcc tgattaattg cacgttcaat
    13441 gaaccgccgc tggccgatgc gccgcagacc gttagctgga agctggaagg tactgactgg
    13501 actatccata accatgcgaa tgtcttctcc cgcaccgggc ttgatatcgg cgcgcgcttc
    13561 tttatgcaac atctgccaga gaatctcgaa ggggagattg tcgatctcgg ttgcggtaat
    13621 ggcgttattg gtctgacgct gcttgataaa aatccgcagg cgaaagtggt gtttgtcgat
    13681 gaatcgccga tggcggttgc ttccagccgt ttgaacgttg aaaccaacat gccagaggcg
    13741 ttggatcgca gcgagtttat gatcaacaac gcgctctccg gcgtggagcc tttccgcttt
    13801 aatgctgtgc tctgcaaccc gccgtttcac caacaacatg cgctgaccga taacgtcgcc
    13861 tgggagatgt tccaccatgc ccgccgctgc ctgaaaatca acggcgagct gtatattgtt
    13921 gccaaccgtc acctggatta cttccataaa ctgaagaaga ttttcggcaa ctgcaccacc
    13981 atcgccacga ataataaatt tgtggtgctg aaagcagtga agctggggcg tcgtcggtaa
    14041 gattgggagt tacctgccgg atgcggcgtt aacgccttat ccggcctacg ttcagcacgt
    14101 tactcccagg ccggataaga cgcggcaagc gtcgcatccg gcaatgtctg cccagttctg
    14161 gaatcgcgct tccataaaac aaaaaatccc tctcaccatc ctctacattc tcttgtttag
    14221 cgtttttcta cgtttattct tccgtcacac agataaattc atacgttgcc aatctgtcaa
    14281 cggatgttat catattgcca atttgtcaac ggacgtgatg aagaatgcac ctgataactc
    14341 aaaaagcatt gaaagatgct gcggaaaaat acccgcaaca taaaacggag ttggtggctc
    14401 tggggaacac gattgctaag ggatatttca aaaaacctga gtcattaaaa gcagtattcc
    14461 catctctgga taacttcaaa tatctggata agcattatgt tttcaatgtt gggggcaatg
    14521 aattacgtgt tgtagcaatg gtcttttttg aatcgcaaaa gtgctacata cgtgaagtta
    14581 tgacgcataa agaatacgat ttctttaccg ctgttcatcg tactaagggg aaaaaatgat
    14641 tgcgattgcc gacatcttgc aagcaggaga aaagctaact gcggtggtac cttttctggc
    14701 gggtattcag aacgaggaac aatacaccca ggcgctggaa ctggtagatc atctgctgct
    14761 caacgatcct gaaaacccct tgctggatct ggtgtgtgcc aaaataaccg cgtgggaaga
    14821 atcagcgccc gaatttgcgg aatttaatgc catggctcaa gccatgcctg gcggtatagc
    14881 cgtgattcgt acccttatgg atcaatatgg tttaaccctt gccgatctgc cggaaattgg
    14941 cagtaaatct atggtgtcac gcgttttgag cgggaagagg aaattaacgc tggaacacgc
    15001 taaaaaattg gcaacgcgat tcggcatttc tcccgccttg tttattgatt aacgcgacgt
    15061 gccggatgcg ttgcatccgg cgcttcaaat aacgatttaa atctccagcg ccagccgtgt
    15121 tccctgggca attgcccgtc gtgcgttcag ctccatagcc acatcgcagc cgccaattaa
    15181 atgcacagtt ttcccgctat caatcagcgg ttgcgccagc gcgcggtttg gctcttgccc
    15241 tgcgcagatc accacattgt ccacagctaa tacctgcgtt tcgccgttga tcaccacatg
    15301 cagcccgtca tcgtcaatct tctgataact tacgcctggg atcattttca caccccgcga
    15361 gagcagggtg gtgcgatgga tccagccggt ggttttgcct aacccctgtc ccggtttgct
    15421 ggctttgcgc tggagcatca caatttgccg tgggctacgc gggatctgca ttccctgcgg
    15481 gcttaagcca ccagcctgtt gtaggctgct gtcgatcccc cactcattac agaacctggc
    15541 gatattctgg ctggttgatt cgcccggctg acttaaatac atcgccgtat caaaaccaat
    15601 cccgccacaa ccgatgatgg caaccttgtt gcctaccggc gctttgtcgc gcagtacatc
    15661 gagataactc aataccttcg gatgatcgat cccgtcgata ggcggaacgc gcggcacgat
    15721 cccactggcg aggatcgttt catcgaacgc ctgtaactga tccgccgtca cggtgtgatt
    15781 gagtttcagc gtcacgcccg tcacttcgat catccggtgg taatagcgca gcgtttcgta
    15841 aaactcctct ttgccgggga tctgtttggc gatattaaac tgcccgccgc tctcgctgtg
    15901 ggcatcaaac aatgttacct gatgcccacg cgccgccgcg ttaatggcaa acgccagccc
    15961 agcaggtccc gcaccgacca ccgccagatt ttttttctgc acggcaggaa ggattggcat
    16021 tttggtttcg tggcaggcgc gaggattcac caggcacgag gtgactttgc caacgaagat
    16081 ctgatcgagg caggcctgat tgcagccaat gcaggtgttg atctcatcgg ctcgccccga
    16141 ttgcgctttt gacagcagtt ccgcatcagc aagaaacggt cgtgccatcg ataccatatc
    16201 ggcatcgccg cgcgagagaa tatcgtcggc aacctgcgga tcgttaatcc ggttggtggt
    16261 taccagcggc agcgagacgt ggcctttcag tttgcgcgtg acccagctaa atgcgccgcg
    16321 cggcacgggc gtggcaatgg tcggaatacg tgcttcatgc cagccaatgc cggtgttgat
    16381 aatggtcgcg cccgccgctt caatggcctg cgccagctct accgtttcgg caaaagtccc
    16441 gccgtcttcg accaggtcga gcatcgacag tcggtagata ataatgaagt cgttgccgac
    16501 gcgttcgcgc accgcacgca ctacttctac ggcaaatcgc atccggttgc ggtaatcgcc
    16561 gccccactgg tcactacgct gattggtgtg cagcgtcaga aattcgttga tcaaataccc
    16621 ttcggacccc attacctcta caccgtcgta tcctgcctcc cgtgccagtt gcgcgcagtg
    16681 ggcgaaatcg tcgatcagtt gcaggatctc ttcatgggtg agttcgtgag gaacgaaacg
    16741 gttgatgggg gcctgcaatg cggagggagc gaccagatgc ggttggtagc tgtagcgccc
    16801 ggtatgcaaa atttgcaggg ctattttgcc gccttcctga tgtaccgctt cggtaatggt
    16861 gcgatggtgt gggatctggc tggcgtcgtt gagcattgcg ccaccttcca tgccaacgcc
    16921 tgttaaatct ggtgcaatgc cgccgctgac aatcagcgcc acgccgtgac gggcgcgttc
    16981 ggcataaaac gctgccagcc gctcggcacc gtccgggtat tcctccagcc cggtgtgcat
    17041 tgagcccatc aacacgcggt tttttaacgt ggtaaaacct aaatccagcg gggcgaacag
    17101 cgacgggtag ctcataaaat tgtccagtat gtaaaataat tgttatgtgg tcggatgagt
    17161 tctaatttag ccgtcgccgg agaaaaggga aaaggggagt gcggggtttg tgatgggatt
    17221 caaaaagaat acgcccggtg gtaacgccgg atgcgacgct tgccgtgttt tatccggcct
    17281 gggggattgc acatgtaggg cggataaggc gttcacgccg catccgccag tggcgcggtg
    17341 cagatgccgg atgcgacgct tgtcgcgtct tatccgacct acgggaacgc acatgtaggg
    17401 cggataaggc gttcacgccg catccgccag tggcgcggtg cagttgccgg atgcaacgct
    17461 tgccgcgttt tatccggcca ggaggattgc acatgtaggg cggataaggc gtttacgccg
    17521 catccgccag cagttgcaaa ttattgctta cggaaaaaat cgttatacaa catatacaaa
    17581 tgcgcggcac tccaggagaa atttggtgcg ccttgctgcg cgccagtcag cgggttgtaa
    17641 ttctcctgaa ttgggccatc ggcggttaat cctttggcgt gccggaagaa cgtatccgcc
    17701 agcttcaggg catcatcacg ataaccgtaa cgctccatcc ctttcagagc aaaccagaac
    17761 tgatccaccc atacgcgccc gcgccagtag atatcagcgc caaaagccgg attggttaac
    17821 gccgccgttc ccagcgggac aaaggtgttg aactctttag gatcgagcat caccttcacc
    17881 accgcgtcgg cattggcctg cgttgccgca ccgttaaaca gcggcgacca gccttccggc
    17941 cctttaccgc gctcaacaat cggtttgccc gcgcagccgt tcgccagtgg tttatcttca
    18001 atacgtacgt catagtagaa ctgggtagtc gggtcgaaca tacaggtgtt gatgtagtcc
    18061 gcgagctgct gtgccaactg gcgatagcgt ttggcctctt ctggcttacc gaggatggtt
    18121 gccatctccg ccagataatg gttatcgctg tacatatagc tggcctgatc caccgactcc
    18181 tgcaatagcg agtagcccag caacgttccg tcctgactgc ggttttcggc gaatttcacc
    18241 gtccagtcgc tacgtttgcc accattagcg acatatttat ccaactgttc tttgtcgata
    18301 aaaccaaaga cggcggcatc atcacgcccc gattcccacg acgcagcgac ctgtgccgga
    18361 atttccagac tgtcgtactg gcctttctcc accacgcggg cgtagttgtt cagcccagac
    18421 tgcgtctctt ctttgttgcc tttcttcacc gcaaacagca tctcgccgct ctcagtgttg
    18481 tgggctttgt cgcgggtcgc gccatattcc ggcacgccgt tgccgttgtg atcgcggtta
    18541 cgtaaccacc agtcgtgata ggcgaccagt ttcgggtaca tctctgccaa ccaggcttta
    18601 tcctgggtga cgttgtacac ttctatcacc gaccaggcgg caaggctggg tttggtatta
    18661 cgttcgttcc agttgccgcc atcgccacca cgctcggggc taaggttcca cgctatcagg
    18721 tcggggacaa agcccacatc ctgcggacgc acgcggtcgc caggctggat ctgccaggag
    18781 aagaccgcgc ggatattctc tttggcgatg tccggattga aatgcgccat cgcaaacgcc
    18841 tgcttctagg tatcccacgg ccaggtctga ttgccggaga accagcgccc ggtcaccgac
    18901 ggtgtaacgg tgttaaattt caccgcaccg cccggtgagc gccagttacc gttgagcgtt
    18961 tcgatcgctt tcaccgcgac gcgcgtctgt tccggcgtcg catccggatt ggttaaccct
    19021 ttcttcaggt attcttccca gcgttgctgc gaggcggtga gataaaacgc cggacgcgcc
    19081 agaatatcgc ggatctgcat ttgctctttg ctaacttcct gagcggtcag cagatgggaa
    19141 taggtggtgt agagcgtggt cgaaccgttg atatgcgcct tactggtaaa gcgattaccg
    19201 ttgatttcag tctgcaccgg cagggattta tgcacctgat attccgattc gccggaggtc
    19261 agcagatccc aggtggcgcg cactttgcca aaggtgactt tcaggccatc acgggtggcg
    19321 ctgattttgc gctgatagtc agggtattcg ccagcaatgg ttttatcgga aagcggtttc
    19381 ccttctttcg cttccagttt ttccagcagt tcgccgtccc acaccagatc cagcggttta
    19441 ttgctggtga ttttggtttc cagtagtgac gtgcgcggcg tggcgaagcg cagagtcatt
    19501 tcgacttgca catcttttgc tgtcagtttt tgcaccagcg caccgggaat actgtatgcc
    19561 tccagcgtaa agtcgacttt cttgccgtcc tgccagacgg tcaggcggtc gaaattgctg
    19621 gccataaagt tgatgtactc ttccgtcagc agcgcaacgc ccggaaagcc gcccatggtg
    19681 ttagggccgt ctggcaacag atgaccatgc caggctccga gatcgaaaaa cggattaaaa
    19741 cgctggtgat cgtcgtaatc gtaatccttc atgtactgcg gcgcgccagt acggttaatc
    19801 acgtttttat aattgtcggc gttagcggca tgggcggaaa aacttatcag cagagcgcag
    19861 gttactggcg ttaaaatagt ttttattttc atgaaattac agtccttatt accagtagaa
    19921 atattgcatc aggaagattt ggtcgctggg atcgtgagag gcatttttca tgtagaagcg
    19981 gctatcaaaa gccgtggcga aacgtccgcc gccgtattcg taccagatac caaactgcat
    20041 gaaagaggtg gtttcatcgt cagcgtggtc agggcgatgt ttggcgtagg tgtaagcggt
    20101 ggagagataa acgcccttta acgcttcata catggcgctc accatcatcc cactttcact
    20161 tcccggtacg tccagcccgt cggcgcgtcc ggtatggtgc caggcgcgag cggcaaagtt
    20221 aggagttagc aggcccgtga gatagagctg tcctgtgccg tcggtgattt ccagaccgtt
    20281 catccaggtg atgccttcgg caaggtcata ctggatgtaa ccgttcatca ttgccggata
    20341 ggtgtatttg tcgtcatagc gatcgtattt gccaaaatgt agccaggctt tgctttcatc
    20401 atgatgtccg gcaggagtcg cggtgacgct ataacgaaag ttgccgctta agttctgtac
    20461 cttcagcgca gccataatgt cgcgggtatt gggaatgacg tagcccagat cgggggtgaa
    20521 atcgccccac cattgcaggt catccagcga ggaatcctaa cgcagactga gcatgatttc
    20581 ggtgccatca ttggtacgat aaccgccata gaaacggtta attccgccct cgaaaccacc
    20641 ccagctatgg tctgg
//
LOCUS       DAJTMC010000065        20603 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.065, whole genome shotgun sequence.
ACCESSION   DAJTMC010000065 DAJTMC010000000
VERSION     DAJTMC010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20603
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.065"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(56..481)
                     /gene="nudI"
                     /locus_tag="OO130_002076"
     CDS             complement(56..481)
                     /gene="nudI"
                     /locus_tag="OO130_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HCR5365493.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVEYGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYTWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            760..1302
                     /locus_tag="OO130_002077"
     CDS             760..1302
                     /locus_tag="OO130_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5365494.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARSTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            1402..2604
                     /locus_tag="OO130_002078"
                     /pseudo
     CDS             1402..2604
                     /locus_tag="OO130_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
     gene            2824..3606
                     /locus_tag="OO130_002079"
     CDS             2824..3606
                     /locus_tag="OO130_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HCR5365495.1"
                     /translation="MLESSKVPALTRAIDILNLIARIGPCSAATIIDTLGIPKSTAYL
                     LLNELRRQRFLSLDHQENFCLWTRLVELSGHALSKMDLRELARPRLTQLMDTTGLLCH
                     LGIIDNGSAYYILKVESSATISVRSHEGKSLSLYRSGIGKCLLSWQPAAVQQSIIEGL
                     VWEQATPTTITHPQQLHEELARIRRQGWSYDNGEDYPDVRCVAAPVFNANNELTAAIS
                     VVGTRLQINEEYRDYLAGKSIACARDISRLLGWKSPFDLQAS"
     gene            3621..4826
                     /gene="rhmD"
                     /locus_tag="OO130_002080"
     CDS             3621..4826
                     /gene="rhmD"
                     /locus_tag="OO130_002080"
                     /EC_number="4.2.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnonate dehydratase"
                     /protein_id="HCR5365496.1"
                     /translation="MTLPKIKQVRAWFTGGATAEKGAGGGDYHDQGANHWIDDHIATP
                     MSKYRDYEQSRQSFGINVLGTLVVEVEAENGQTGFAVSTAGEMGCFIVEKHLNRFIEG
                     KCVSDIKLIHDQMLNATLYYSGSGGLVMNTISCVDLALWDLFGKVVGLPVYKLLGGAV
                     RDEIQFYATGARPDLAKEMGFIGGKMPTHWGPHDGDAGIRKDAAMVADMREKCGEDFW
                     LMLDCWMSQDVNYATKLAHACAPYNLKWIEECLPPQQYEGYRELKRNAPVGMMVTSGE
                     HHGTLQSFRTLSETGIDIMQPDVGWCGGLTTLVEIAAIAKSRGQLVVPHGSSVYSHHA
                     VITFTNTPFSEFLMTSPDCSTMRPQFDPILLNEPVPVNGRIHKSVLDKPGFGVELNRD
                     CNLKRPYSH"
     gene            4883..6172
                     /locus_tag="OO130_002081"
     CDS             4883..6172
                     /locus_tag="OO130_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5365497.1"
                     /translation="MSTALLDAVVKKNRVRLIPFMLALYVLAFLDRSNIGFAKQTYQI
                     DTGLSNEAYALGAGIFFVVYAFLGVPANLLMRKLGARTWIGTTTLLWGFLSAAMAWAD
                     TEAKFLIVRTLLGAAEAGFFPGMIYLTSQWFPQRNRASIMGLFYMGAPLALTLGSPLS
                     GALLEMHGFMGHPGWFWMFVIEGLLAVGAGVFTFFWLDDTPEQARFLSKQEKTLLINQ
                     LASEEQQKVTSRLSDALRNGRVWQLAIIYLTIQVAVYGLIFFLPTQVAALLGTKVGFT
                     ASVVTAIPWVAALFGTWLIPRYSDKTGERRNVAALTLLAAGIGIGLSGLLSPVMAIVA
                     LCVAAIGFIAVQPVFWTMPTQLLSGTALAAGIGFVNLFGAVGGFIAPILRVKAETLFA
                     SDAAGLLTLAAVAVIGSLIIFTLRVNRTVAQTDVAHH"
     gene            6190..6993
                     /gene="yfaU"
                     /locus_tag="OO130_002082"
     CDS             6190..6993
                     /gene="yfaU"
                     /locus_tag="OO130_002082"
                     /EC_number="4.1.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxy-L-rhamnonate aldolase"
                     /protein_id="HCR5365498.1"
                     /translation="MNALLTNPFKERLRKGEVQIGLWLSSTTSYMAEIAATSGYDWLL
                     IDGEHAPNTIQDLYHQLQAVAPYASQPVIRPVEGSKSLIKQVLDIGAQTLLIPMVDTA
                     DQARQVASATRYPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESKTALDN
                     LDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAAGFLAV
                     APDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(7034..7936)
                     /locus_tag="OO130_002083"
     CDS             complement(7034..7936)
                     /locus_tag="OO130_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HCR5365499.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIEEIAERSPLQKERLMTIAERLRQEGHQIGWQEGMHEQAIKIALRMLE
                     QGIDRDQVLAATQLSEADLAANNH"
     gene            complement(8129..9319)
                     /gene="glpC"
                     /locus_tag="OO130_002084"
     CDS             complement(8129..9319)
                     /gene="glpC"
                     /locus_tag="OO130_002084"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HCR5365500.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRKIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            complement(9316..10575)
                     /gene="glpB"
                     /locus_tag="OO130_002085"
     CDS             complement(9316..10575)
                     /gene="glpB"
                     /locus_tag="OO130_002085"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HCR5365501.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVADIHSGLESLRQQAPAHPYSLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLRSTWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLH
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLLCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPHFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            complement(10565..12193)
                     /gene="glpA"
                     /locus_tag="OO130_002086"
     CDS             complement(10565..12193)
                     /gene="glpA"
                     /locus_tag="OO130_002086"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HCR5365502.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            12466..13824
                     /gene="glpT"
                     /locus_tag="OO130_002087"
     CDS             12466..13824
                     /gene="glpT"
                     /locus_tag="OO130_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HCR5365503.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFLYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            13829..14905
                     /gene="glpQ"
                     /locus_tag="OO130_002088"
     CDS             13829..14905
                     /gene="glpQ"
                     /locus_tag="OO130_002088"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCR5365504.1"
                     /translation="MKLTLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMELNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTPDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(14947..15189)
                     /locus_tag="OO130_002089"
                     /pseudo
     CDS             complement(14947..15189)
                     /locus_tag="OO130_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            15368..16018
                     /gene="inaA"
                     /locus_tag="OO130_002090"
                     /pseudo
     CDS             15368..16018
                     /gene="inaA"
                     /locus_tag="OO130_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416740.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
     gene            complement(16072..16326)
                     /gene="yfaE"
                     /locus_tag="OO130_002091"
     CDS             complement(16072..16326)
                     /gene="yfaE"
                     /locus_tag="OO130_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HCR5365505.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEM"
     gene            complement(16326..17456)
                     /gene="nrdB"
                     /locus_tag="OO130_002092"
     CDS             complement(16326..17456)
                     /gene="nrdB"
                     /locus_tag="OO130_002092"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HCR5365506.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDSEVDTDDLSNFQL"
     gene            complement(17603..19888)
                     /gene="nrdA"
                     /locus_tag="OO130_002093"
     CDS             complement(17603..19888)
                     /gene="nrdA"
                     /locus_tag="OO130_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HCR5365507.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINNLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGECP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
BASE COUNT         4844 a         4992 c         5525 g         5242 t
ORIGIN      
        1 aattcgttaa ccactaaatt ttcaatctga cagccagtga tggctgtcat cattgttaaa
       61 gaagaccttt caaacgtaac gtttttcggg tggcaacatt caaatcataa tgcaccagat
      121 cttcaggttt tacccacgtg tagtcctgaa actcttcgtt tattttcact tctcggttgg
      181 cagaaacgca gtcaaaaatc aggtaaatca tataaatctc ttccttgcga ccatctgcat
      241 acgtcttggt gcgaatatca tcgctgaagg tccacggcgt gatttctgtc aaaagcagct
      301 gttctcccag ttcttcgcga atttcgcggc gtagtgcctc ttcaattcgt tcgccatact
      361 ccacgccgcc acccgaaagc gcccattgac cggggaaaac gccgcgatcg tcggccattt
      421 tgcacagcag ataagcacca tcattttgaa ttaaagggca tacaatagtc cgttgtcgca
      481 caggggtatt ccttataaat aaaaagaaac catgagaata cggtaactca gaaatatgtg
      541 aaagtgacag taatctcttt tgcgatgata actgcactta ttattatttt tgtgtgtatc
      601 ggtaaagtgt gtttattttt tcggcgttta attatttaag aagtatttag ttacaataag
      661 tgactagatc ccttttttcg ctcattgaat cctctgtgcc aggcagtaca atcccgcagc
      721 tttgggaatt ttgattaatt gcttgtggga aaggggataa tgaaaaaaat tgcgctagca
      781 ggtctggccg gaatgctgtt ggtttctgca tcggtcaatg caatgagcat cagcggtcag
      841 gcgggtaaag aatacaccaa tattggtgtc ggttttggga ctgaatcgac gggcctggcg
      901 ttaagcggta actggacaca taacgacgac gacggtgacg tcgctggcgt ggggctgggg
      961 ttgaatctgc ctctagggcc gttaatggcg accgttggcg gaaaaggcgt ttacaccaac
     1021 ccgaattacg gcgatgaagg ttatgccgca gcggtaggag gtggtttgca gtggaaaatt
     1081 ggcaacagct tccgtttgtt tggcgagtat tactactctc cggattcact ctccagcggt
     1141 attcaaagtt atgaggaagc gaatgctggc gcgcgttcca ccattatgcg tccagtcagt
     1201 attgaggcgg gttatcgcta cctgaatttg tcgggtaaag atggtaaccg cgacaacgcc
     1261 gtggctgacg gcccgtatgt tggggttaac gccagtttct gatcccttcc ggcgcggcat
     1321 tctgtcgcgc cattcagttt cttactacgc cttatctccc tttgcgttat agtgattcga
     1381 taacaaaagc aggagataac catgttaaaa gtggaaatgt tatccaccgg ggatgaagtg
     1441 ttacacgggc aaatcgttga cactaacgct gcctggctgg ccgatttttt ctttcatcag
     1501 gggctgccat tatctcgcct caatacggtg ggggataacc ttgatgactt agtcaccatt
     1561 cttcgcgaac gtagtcagca cgccgatgtg ctgatcgtta acggcgggct gggaccgacc
     1621 agcgatgatt taagcgcact cgccgctgcg acagcaaaag gtgaaggcct ggtgctgcat
     1681 gaagcctggc tcaaagagat ggaacgctat ttccacgaac gtggacgagt aatggcaccg
     1741 agcaaccgta aacaagcgga gctgcctgcc agtgctgaat ttatcaataa cccggtaggc
     1801 accgcctgtg gttttgccgt gcagcttaat cgttgcctga tgttctttac tcccggcgta
     1861 ccgtcagaat ttaaggtgat ggtcgagcac gaaatcctgc cgcgcctgcg cgagcgtttt
     1921 tctttaccac agccgccggt ttgtctgcgt ttaactacct ttggtcgttc ggaaagcgat
     1981 ctggcacaaa gcctggatac tctacaactg ccgccgggcg taacaatggg ctatcgctct
     2041 tcaatgccga ttatcgaact gaaactcacc ggaccggcaa gcgagcaaca ggcgatggaa
     2101 aaactgtggc tggacgttaa acgtgttgcc ggacagagcg tgattttcga aggcaccgaa
     2161 ggactgcccg tgcagatcag tcgcgaattg caaaaccgcc agttcagcct gacgttgagc
     2221 gagtaattca ccggtggttt attggctttg caactttctc gcgcaggtgc tccattgctg
     2281 gcgtgtgaag tggttccttc acaggaagaa aacctggcgc aaactgcgca ctggattaca
     2341 gaacggcggg ccaaccattt tgccgggctg gcactggctg tttcgggttt cgagaacgaa
     2401 catctcaact ttgcgctagc cacaccagac ggcactttcg ctctgcgtgt gcgtttcagc
     2461 actacgcgct acagcctggc tatccgtcag gaagtgtgcg caatgatggc actgaatatg
     2521 ctgcgccgtt ggttaaacgg ccaggatatc gccagtgagc atggctggat tgaggttatt
     2581 gagtccatga ccttatctgt ctgattccgc cactctgtgc cggtttactg caccggcaca
     2641 ttccccctcc ggcaaaactt gtccaacctg ttgcactcaa taagaaaaaa tccgtgcact
     2701 gattcacaaa tttgtgcgta atttctccag tccgctggac gaggagcggg atttgatgcg
     2761 cttcacaatt ttactccccc tgagcaacga gactgattcc agtatctaag actggagtcc
     2821 agtatgttgg aatcaagcaa agttcccgcc ctgacacggg ctattgatat tctcaatttg
     2881 attgcccgca ttggcccctg tagtgctgcg acgatcattg acacattggg aatccctaaa
     2941 agcacggcct atttgctgct taatgaactc aggcgtcagc gttttcttag ccttgatcac
     3001 caggaaaatt tttgtttgtg gaccaggctg gtggagcttt ctggtcatgc gttaagcaag
     3061 atggatctcc gggaactggc tcgaccgcgt ttgacgcaac tgatggatac caccgggctg
     3121 ttatgtcatc tggggattat tgataacggc agcgcttatt acattttaaa agtggagtca
     3181 tcagccacta tcagcgtgcg ttcccatgaa ggaaaaagcc tttcgcttta tcgctccgga
     3241 ataggtaaat gcctgctttc ctggcaacct gcagcggtac agcaaagtat tatcgaaggg
     3301 ctggtctggg agcaggcgac acccacaacg attactcatc cacagcaact tcacgaagaa
     3361 ctggcgcgta ttcgccgtca gggctggagc tatgacaacg gcgaagatta ccccgatgtc
     3421 cgttgtgtcg ctgcgccggt gtttaacgcc aataacgagc ttaccgctgc gatctcggtg
     3481 gtcggtaccc gcttacaaat caatgaagaa taccgtgatt atctggcggg caagtccatt
     3541 gcctgcgcca gggatatttc acgtctgttg ggatggaaaa gtccctttga cttacaagcc
     3601 tcataacaat ggagaacatc atgaccctac caaaaattaa acaggttcgc gcctggttta
     3661 ctggcggtgc gacagcagaa aaaggcgctg gcggcggtga ttatcacgac cagggggcga
     3721 atcactggat agacgatcat attgccaccc cgatgagtaa ataccgcgat tacgagcagt
     3781 cacgccagtc atttggcatt aacgttcttg gcacgttggt tgttgaagtc gaagcagaaa
     3841 acggccagac cggattcgct gtttcgacag ccggtgaaat gggctgtttt atcgtcgaaa
     3901 aacaccttaa ccgtttcatt gagggtaaat gtgtcagtga tatcaaactg atccacgatc
     3961 aaatgctcaa tgccaccctg tattactccg gctctggtgg cttggtgatg aatacgattt
     4021 cctgtgtcga tctggctctg tgggatctgt tcggcaaagt ggtcgggctt ccggtttata
     4081 aacttttagg cggcgctgtt cgtgatgaga ttcagttcta cgccacaggt gcgcgtccgg
     4141 atctggcaaa agagatgggc tttatcggtg gcaaaatgcc gacgcactgg gggccacatg
     4201 atggcgatgc ggggatccgc aaagatgccg ctatggtcgc ggatatgcgt gaaaaatgcg
     4261 gtgaagattt ctggttaatg ctcgactgct ggatgagtca ggacgtgaac tatgcgacca
     4321 aactagccca cgcttgcgcg ccctataacc tgaaatggat cgaagagtgc ctgccgccac
     4381 agcagtatga aggttatcgc gaactgaaac gcaacgcgcc agtcggaatg atggtcacca
     4441 gcggtgagca ccacggcaca ctgcaatctt ttcgtacgct ttcagaaacc ggtatcgaca
     4501 ttatgcagcc ggatgttggc tggtgcggtg gtttaaccac gctggtggaa attgccgcaa
     4561 tcgccaaatc cagggggcaa ctggtggtgc cgcacggttc gtctgtttac tctcaccatg
     4621 cggtgatcac cttcaccaat acgccattca gcgaattcct gatgaccagc ccggattgtt
     4681 caacgatgcg tccgcagttt gacccgattc tgctcaatga gccggttccg gtgaatggtc
     4741 gtattcataa atcagtgctt gataaacccg gttttggcgt cgaactcaat cgtgactgca
     4801 atttgaaacg cccctacagc cactaatcac tccgggcgtt gctgtgacaa cggtaacgcc
     4861 cacatattct aaggagcttt ttatgagcac cgctttgctt gacgccgtgg tgaagaaaaa
     4921 ccgtgtgcgt ttaattccgt ttatgttggc gctgtatgtg ctggcgtttc tcgaccgttc
     4981 gaatatcggt tttgccaaac agacctacca gattgatacc gggttgagta atgaagctta
     5041 tgcgctggga gcaggcattt tctttgtggt atatgcgttt ctgggtgttc cggcgaatct
     5101 tttgatgcgc aaactggggg ccagaacctg gattggtacg acaacactgc tgtggggatt
     5161 tctttcggca gccatggcat gggccgatac tgaagcgaaa tttctgatag ttcgcactct
     5221 gcttggtgct gcggaggccg gatttttccc tggtatgatt tatctcacct cgcaatggtt
     5281 tccgcagcgt aatcgcgcca gcattatggg gctgttctat atgggcgcac cgctggcgtt
     5341 aacactggga tcaccgcttt ccggcgcgct gttggagatg catggattta tggggcatcc
     5401 cggctggttc tggatgtttg tgattgaagg attgttggca gtcggtgctg gggtattcac
     5461 attcttttgg cttgatgaca caccggagca ggcacgtttt ctgagtaaac aagaaaaaac
     5521 gttgcttatc aatcaactgg caagtgaaga acaacagaaa gtgacttctc ggctgagcga
     5581 tgcgctgcgt aatggccgag tctggcaact agcgattatc tacctgacca ttcaggtggc
     5641 ggtttacgga ttaattttct tcctgccgac ccaggttgcg gcattgctgg gaacaaaagt
     5701 gggctttaca gcgtcggtgg tcaccgccat tccgtgggtt gcggccttgt ttgggacctg
     5761 gcttattccg cgctattccg ataaaaccgg cgaacggcgt aatgtcgcag cgctgacatt
     5821 actggcggcg ggcattggta ttggtctgtc cgggctgctt tctccagtaa tggcgatcgt
     5881 agcgctgtgt gttgcagcta tcgggtttat tgccgtgcag ccggtgttct ggacgatgcc
     5941 gacacaactg ctttccggta cggcgctggc tgcggggatt ggttttgtaa acctgtttgg
     6001 tgcagtgggc gggtttattg ccccgatcct gcgcgtgaaa gcagaaacgt tatttgccag
     6061 cgatgcggca ggattactga cactggcagc ggtggcggtc atcggttcgc tgattatttt
     6121 cactctgcgt gtaaatcgta ctgttgcgca gaccgacgtg gcacatcatt aaataggtta
     6181 aggaacacga tgaacgcatt attaaccaat ccctttaaag aacgtttacg caagggcgaa
     6241 gtgcaaattg gtctgtggtt aagctcgact acttcttata tggcagaaat tgccgctact
     6301 tccggttatg actggttgct gattgacggg gagcacgcgc caaacaccat tcaggatctt
     6361 tatcaccagc tacaggcggt agcgccctat gccagccaac ccgtgatccg tccggtggaa
     6421 ggcagtaaat cgctgattaa acaagttctg gatattggcg cgcaaactct attgatcccg
     6481 atggtcgata ctgccgatca ggctcgtcag gtggcgtctg ccacgcgcta tcctccctac
     6541 ggtgagcgtg gtgttggggc cagtgtggca cgggctgcgc gctggggacg cattgagaat
     6601 tacatggcgc aagtcaacga ttcgctttgt ctgttagtgc aggtggaaag taaaacggca
     6661 ctggataacc tggacgagat cctcgacgtc gaagggattg atggcgtgtt tattggacca
     6721 gcggatcttt ctgcgtcgtt gggctacccg gataacgccg ggcacccgga agtgcagcga
     6781 attattgaaa ccagcattcg gcggatccgt gctgcgggta aagcggctgg ttttctggct
     6841 gtggctcctg atatggcgca gcaatgcctg gcgtggggag cgaactttgt cgctgttggc
     6901 gttgacacga tgctctacag cgatgccctg gatcaacgac tggcgatgtt taaatcaggc
     6961 aaaaatgggc cacgcataaa aggtagttat tgatatcaaa ggcccatggg gatcggctgt
     7021 gggcctgtgt taattagtgg ttattcgctg ccagatcggc ttcgcttagc tgggtggccg
     7081 cgagcacctg gtcacgatca atgccctgtt ccagcatgcg caaagcaatt ttaatggctt
     7141 gttcatgcat accttcctgc cagccaattt gatgcccttc ctgccgtagc cgttcagcaa
     7201 tagtcattaa tctctccttt tgtagtggtg aacgttcggc aatctcctca ataaaacggg
     7261 tgaaacggga ggtatcgccg cattgcagca gataattaaa cagtgtttgt agctggctgt
     7321 cattagtgaa gcctctaacc aaaagcgtgg tgatcctgtc gaccatgccg attaaatcgc
     7381 ggtcgcgaat atgcttttga atcagttcca gcagagctat acgccgatgt tgcatgatct
     7441 catcgtcagg tacgatggtg atatccacca acggaaaagc ttcggtgtac aactgccggg
     7501 caagttgcgg atcgtcaaac tcatccagcc agttaagtga gtacgggtag ggcgatgttt
     7561 cgccatgata aaacagcaat ggcaccacca gcggaactct gtcatagcct ttatccaggt
     7621 gacgctgcat ggcggcagtg gcatagcgca ttagccgaaa agccatattc ttttctgcag
     7681 agctttgatg ttcaatcacg cagtagatat agccgtcacc gtcgctggtt tccacggacc
     7741 acaaaacatc cgagtagtaa gcgcgtaaac ttttttcaat aaaactagtg ggttccaggc
     7801 gtaaggtttg caggttgcaa agcttgcgca gtggttctgg taaatgtatt tcgagaaaat
     7861 cccgtgcggt ttcgggtgtg aacataaagg ttttaaatac cgcatcatgc ggggaggagg
     7921 ttgttgattc ggtcatggtt gtccgtcacc tgatatttat gatgacgcga cattacggtt
     7981 atcctaggcc tttgacatct gccagtttca gcctttgcga gacgttttca gaggaatttt
     8041 cactgcgttg caacgcatac attgggcacg gaatcgtgta cgcaaaaatg cccggtaacc
     8101 acatgcttac cgggcatcag aaaggagttt aagccagcgc ctgggccagt agcgtaatcg
     8161 gatgttcgca gcgaagactt gtggacatct caatctgcca tttacaggtt tcgcagtcgg
     8221 tgaccaccag atctgcgccg ctttcttcta tctggcggaa cagtggtgcg ccgatggctt
     8281 gtgaggtggg gtagttctct tttttgaaac cgtaagtacc cgcaataccg cagcactggg
     8341 aatccagcac cgttaactca agccccggga ttttacgcaa cagctccagg gtgtagagcg
     8401 tccagcccat tttttccata tggcacggag tgtgataaac cactttcagc ggcagcggtt
     8461 tcagcggtaa cgttttgcct tcgtccagct tgcgccacag ccagcgggtt gccagttcga
     8521 tatgatcgcg caagcctttg ttgtcgacat tcagcacttc cgggtattcg tcgcgcaggg
     8581 caaatgtaca ggttgaggag gtggcaatca ccggaatgcc ttttactccc acagcttcgc
     8641 ggatcgactc tacattcgta attgcctgtt tgcgtgcttt atcggtaaag ccgttggcga
     8701 ttagcggtac gccgcagcat ttttctttgc tgagcagttg tacaccggta cccattgcgt
     8761 tgagcacttt aattaaatct ttacctaact gcggatggtt gtagttaacg aagcagccgt
     8821 gaaagaaagc gacctggtct ttatattgtg cttgctgagc cgctatgctg cgataccagc
     8881 gacggaacgt gccgaaggag tatttcggta gcgtgcgacg atgatcgatt tttaacgccg
     8941 catcaagcag ctgccgcacc ggtttcagcg aggtagcggt gttgacgatt ggtgcgaacg
     9001 gcgtggaaac gctacccatc aggtcggtat gactcaacac aaaattacgc agcgacgggc
     9061 gcgtggtgtc atattttgcc cgcgcgcgct ggataatatc gccaatcttc acatcggacg
     9121 ggcaggcgac ttcacaacgt ttgcagttga tgcaatattt cagcgcctcg tcatacagtg
     9181 cgccatcttt caaacgcaga cgctcgccat ccggcccggc ttgttttggc cctggataac
     9241 cgggattcac ccggctcacc gggcaggcgg tggtgcagac ggtgcactta atgcagtttt
     9301 cgaagctggt gtcgttcatt gttggcctcc tgcgcgttgg gcaatctgtt gagcggcatg
     9361 taaagcactg acggcacaaa caccgccgcc gcatccctgg gcgatgggat caaatccgcc
     9421 cagcaccgaa ccgatggcaa acaggttctc aatggtttgc cctgcctgcg acgggcgtag
     9481 cgtctcatca gtggttacac cgaactgctg ccacggttgc ggcgcaaaaa aatcgccctt
     9541 ataccattca ccccgcgtgg cggtttgtag cacatcaagg ccgagaatcg gctcgcgaat
     9601 gccgttacgt tccgctacca gtccgccact aaagaaactg ccgctggcga gaaccgcgaa
     9661 atgtggacgt agcggaatat cggcgtgatt gcgggtccag atttcattca ctacgccatt
     9721 tttacaggtc acttttttca cttcatcgcc cggcatccac acgccaccct ggcgcacaaa
     9781 ctggcgctgt aactggtttt gcagacgaat gcccagcacg gaaggcggca gcgttggcaa
     9841 aagcatcagt gaacaaagta gtttttcatt caaccaacgc cacagtttgt catcggccag
     9901 gccgaagcag gcaggcatca ggatcatttc gcaggtattg gcgacaggaa taagcgcatc
     9961 aagtaacagc ggccagtttt cttcattatc aaggaaacgg gcgatattca cagcgcgaaa
    10021 ttcggtggcg ttattgcgca ggacatccag ttccggcagc tctatttctg cggtttcaac
    10081 cgaaagatcc agttcgtgca acgaagccgc cgcaagatgc gcctgaaaat ccatcaggcc
    10141 gcttatcccg accacacata ttttcttcgc gggcaacggc cagacgggga cttctggcga
    10201 acttagccag gtagagcgca gagtgccgag cggcgtaatc cgctggtgag ccagttctac
    10261 gctgccctgc aattgcgcac cgctttcagc gattaatgcc tgcgcctggc aagcgagatc
    10321 gagcacgcgt tgtggcccga gaagagagta aggatgtgca ggtgcctgct gacgcaaaga
    10381 ttccagtcca ctgtgaatgt ctgccacggg ttgaccatct ggcagatggc tcagcaaatc
    10441 cagcgaaccg gatgagaaat gcaacgcgct ttgaccacgc gtgacaatgg cacagcgcag
    10501 gccgtgtttt tgcagttgca ggccacagag taatccggcg aggccgccgc ccataatgac
    10561 agtatcaaag cgcatctttc tgctccttct ccagaccaca cagtccctga taaacccagc
    10621 gggtaaattc gctttcgcgc agtgcgtctc cccaggcgat gggttgcacg cctttccagc
    10681 gttcgttaag gaaggtggaa agttgctcga tagattgcgc ggacgtcgtg acgttaaacc
    10741 gttgcagcag tccggcagcg cggcaggcgc agagttcgcc ctggcaggtg cccatcccca
    10801 cacgggtacg acggcgtaaa tccagcaggc tattaacgtt taaattttct accgcgtact
    10861 gcacttcacc cgcagttacc gcttcgcact cacataccag gctacggtgc agacggcctt
    10921 cgctcagcca ggcaggcgtg cgatcgccat gacgataaac cgcagaaccg cgcagcgggg
    10981 caggcaggga gatgacttta cgcaaggtaa cttcagcggg ttcttgtgaa ccaggcagtg
    11041 ccagatcggc agtcgtacag gggcgcgtgt tgcccagttt gcggcatacc gcgtcggtag
    11101 cccattcagc catcagccga taggtcatca gtttgccacc ggtgatggtg ataaatccgt
    11161 ccagaccatc gcgttcagca tggtcgagca gcacgatgcc acggctgacg ttacgtccgc
    11221 tcggatcgtc atcgctggca accagcgggc gcacgccaga ataggcccgc aaaatgcgcg
    11281 ttttcgccat cacgggggcc agtttttccc cttcacgcag cagaatatca acctcttctg
    11341 ccgtcactcg attatcgtca atctcgttgt aatcaatacg taaagaggtg gtaccaatca
    11401 gcgaaatggt atcgccaggc accagaatat cggcgtcgga aggtttacgg cagcggttga
    11461 tcacatgctg gttaatgcgg tgatccatga tcagcagcga tcctttcgcc gggaacatgc
    11521 gaatgcgcag atcggcatat tcggcaatgt gttgccccca gatcccagcg gcattaacca
    11581 cgacaggtgc atgaagggcc tgagtttcgc cggtgagatg gttacgtaca cgaacaccgc
    11641 acaccgtcgc gccttcacga atcagccccg tgacttcatg agcggtaagg ataacggcac
    11701 cgtgttcttt ggcatccagc atgtttgctg cggtcagacg aaatggatca acggtgccat
    11761 ccggaacttt caccgcgcca atcagtgccg ggttaacggc aggttcgata atgcgcgctt
    11821 gctgcgggtc tatagcttct gcgctgatcc ctgcttcttc gcaggcgcga ataaaagtgg
    11881 cctggaatga gaggtcatct tccggcaggg tgataaacag gccgttggtt ggttcaacgc
    11941 agtgacgtgc aatgcgtttc aggatctggt tttcactaat gcattcgcgg gccgattccg
    12001 catcggttac cgcatagcgc gcaccgctgt gcagcaggcc gtggttacgc ccggtggcac
    12061 cggttgcgat gtcgtggcgc tcaaccaaaa tcacgcgcag cccgcgcagg gcacagtcgc
    12121 gggcaatccc ggctcccgtt gcgccgccgc caatgataat cacgtcactt gattgcgagt
    12181 cgcgagtttt cattgttatc cctctgaagt tcgtttttta ccatttagcc atagtaaaaa
    12241 catgaattgt ttgatttcgc gcatgttcgc tcataattcg aaagtgaaac gtgatttcat
    12301 gcgtcatttt gaacattttg taaatcttat ttaataatgt gtgcggcaat tcacatttaa
    12361 tttatgaatg ttttcttaac atcgcggcaa ctcaagaaac ggcaggttct ctcactgaat
    12421 caggctgtta atcataaata agaccacggg ccacggaggc tatcaatgtt gagtattttt
    12481 aaaccagcgc cacacaaagc gcgcttacct gctgcggaga tcgatccgac ttatcgtcga
    12541 ttgcgctggc aaattttctt ggggatattc tttggctatg cggcttacta tttggttcgt
    12601 aagaactttg cgcttgctat gccttatctg gtcgagcagg gattctctcg cggtgattta
    12661 ggtttcgcgc tttcggggat ctcgatcgct tatggatttt cgaaattcat aatgggttcg
    12721 gtatcagatc gctcgaatcc gcgcgttttc ctccccgcag gtttgattct ggcggcggca
    12781 gtgatgttgt ttatgggctt tgtgccatgg gcgacgtcga gcattgcggt gatgtttgta
    12841 cttttgttcc tctgcggttg gttccagggg atggggtggc cgccgtgtgg tcgtactatg
    12901 gtgcactggt ggtcgcagaa agaacgtggc ggcattgtgt cagtgtggaa ctgtgcgcac
    12961 aacgttggtg gtggtattcc gccgctgctg ttcctgctgg ggatggcctg gttcaatgac
    13021 tggcatgcgg cgctctatat gcctgctttc tgcgccattc tggtggcatt attcgccttt
    13081 gcgatgatgc gcgatacccc gcaatcctgt ggcttgccgc cgatcgaaga gtacaaaaat
    13141 gattatccgg acgactataa cgaaaaagcg gaacaggagc tgacggcgaa gcaaatcttc
    13201 atgcagtacg tactgccgaa caaactgctg tggtatatcg ccatcgccaa cgtgttcgtt
    13261 tatctgctgc gttacggcat cctcgactgg tcaccgactt atctgaaaga ggttaagcat
    13321 ttcgcgctgg ataaatcctc ctgggcctac ttcctttatg aatatgcagg tattccgggc
    13381 actctgctgt gcggctggat gtcggataaa gtcttccgtg gcaaccgtgg ggcaaccggc
    13441 gttttcttta tgacactggt gaccatcgcg actatcgttt actggatgaa cccggcaggt
    13501 aacccaaccg tcgatatgat ttgtatgatt gttatcggct tcctgatcta cggtcctgtg
    13561 atgctgatcg gtctgcatgc gctggaactg gcaccgaaaa aagcggcagg tacggcagcg
    13621 ggctttaccg ggctgtttgg ttacctgggc ggttcggtgg cggcgagcgc gattgttggc
    13681 tacaccgtgg acttcttcgg ctgggatggc ggctttatgg taatgattgg cggcagcatt
    13741 ctggcggtta tcttgttgat tgttgtgatg attggcgaaa aacgtcgcca tgaacaatta
    13801 ctacaaaaac gcaacggagg ctaatggcat gaaattgacg ctgaaaaacc ttagcatggc
    13861 gatcatgatg agcactatag tcatgggaag cagtgcaatg gcggcggaca gcaacgaaaa
    13921 aatagtcatc gcccatcgcg gtgccagtgg atatttgccg gagcatacgc tgccagcaaa
    13981 agcgatggcg tatgcgcagg gagcggatta tctggaacag gatttggtga tgaccaaaga
    14041 cgaccatctg gttgttctgc atgaccatta tctcgatcgt gttactgatg ttgccgatcg
    14101 tttcccggat cgggcgcgca aagacggtcg ttactacgcg atagatttca cgctggatga
    14161 aattaagtcg ttgaaattta ccgaaggttt cgatattgaa aacggtaaaa aagtgcagac
    14221 ttatccgggg cgtttcccaa tgggtaagtc cgacttccgg gtgcacacct ttgaagaaga
    14281 gattgaattt gttcaggggt taaatcactc taccgggaaa aatatcggta tttatccaga
    14341 aatcaaagcg ccgtggttcc atcatcagga agggaaggat attgcggcaa aaacgctgga
    14401 agtgctgaag aaatatggtt acaccggtaa agacgataaa gtttatttgc aatgttttga
    14461 tgctgatgag ctgaagcgta ttaagaatga gctggaaccc aaaatgggca tggagctcaa
    14521 tctggtacag ctgattgcct ataccgactg gaatgaaacg cagcagaaac agccggatgg
    14581 aagctgggtt aattacaact acgactggat gtttaagccg ggtgccatga aacaggtggc
    14641 ggaatatgca gatggtattg gtccggatta ccatatgttg attgaggaga catcgcagcc
    14701 gggtaatatc aaactcactg gcatggtgca agatgctcag cagaataaac tggtagtgca
    14761 tccttatacc gtgcggtcag ataaactgcc tgaatacact cctgatgtga atcagttata
    14821 tgatgctctg tataacaaag cgggtgtaaa tgggctgttt actgatttcc ctgataaggc
    14881 agtaaaattt cttaataaag agtaatgtta ctgcaggatg gcgttcatgc gccatcctgt
    14941 ctcatatcaa atttttccaa tcgccactaa ttgttccatg cacatttgta gtaaaaacag
    15001 tggactgcct tctaccggct tttctttagc cagcaacgaa atctgtcgat agaaagttgg
    15061 ttcgagagga acggaacaca attcacctgc aatgcttttc agcgttaact ctggttgtaa
    15121 ggcgatgcct aatccctgtc gaataaaatt catcgctgta tccgcctgat taaattcaca
    15181 ttttatctgc tgggcgcact attagtggta gtggctggtg gaatctatgc gctgcaaatg
    15241 aagggtaaaa gcggtgtcaa gctagtagtg gcaaaagaaa ttcattaata cgacacgttt
    15301 cattaagatt ttcctcaggt aaccacctat agtcattgtc gttaataaaa acgacaggcg
    15361 atagggtatg gcagtttcag caaagtacga cgaatttaat cactggtggg caacagaggg
    15421 cgactgggtt gaagagccta actatcgccg taatggtatg agcggggtgc aatgcgtcga
    15481 gcgcaacggc aaaaagctgt atgtaaagcg catgacgcat catctgtttc attctgtacg
    15541 ttatccgttc ggccgaccaa cgattgtccg tgaggttgcg gtaattaaat aactggaacg
    15601 ggccggtgtc atcgtgccga agatcgtttt tggtgaagcg gtgaaaattg agggtgaatg
    15661 gcgagcgttg ctggtgactg aagatatggc ggggttcatc agcattgctg actggtatgc
    15721 ccagcatgca gtatcgcctt attctgacga agtacggcaa gccatgttga aagcagtggc
    15781 gctggcgttt aagaaaatgc atagcattaa tcgtcagcat ggctgttgtt atgttcgcca
    15841 tatctatgtg aaaacagaag gtaaggcaga agctggtttt cttgacctgg agaaaagtcg
    15901 tcgccgcttg cgtcgcgata aagcgattaa tcatgacttc cgccagctgg aaaaatatct
    15961 ggagccaatc cccaaagcgg actgggagca ggtgaaagcg tattactacg ccatgtaatc
    16021 gttgtaggcc agataagaca cataagcgtt gtatctggca ctacaaccaa ttcacatctc
    16081 gatttcaata tcgccttttg cccggcaaca acagggcaaa atttcccccg gctgaataaa
    16141 ggctaacggt tcggcaatcc agtcaacttg acctgcaacc aggcgtgtgc gacaggagcc
    16201 gcagtaacct tcgcgacact ggtactcaac cgccacattg tgggattcca gcgctgccag
    16261 aagggaaggg tgttcatcct ggcacagcag ttgtgtgcca gtgatgcgca gggtaacgcg
    16321 ggccatcaga gctggaagtt actcaaatcg tcggtgtcca cttccgagtc aatctgcccg
    16381 accagataag aactgacttc cacttcctgc ggagcaacct gcacgttatc agacaccagc
    16441 caagtgttga tccacgggat cgggttggag cgcgtctgga acggcagatc caaaccgact
    16501 gcctgcatac ggatattggt gatgtattca acgtactggc agagaatgtc tttattcaga
    16561 ccaatcatcg aaccgtcgcg gaacagataa tccgcccagt ctttctcctg ttgagctgcc
    16621 tgaacaaaca ggtcatagca ctcctgctta cactcttcgg caatttccgc catctcagga
    16681 tcgtccgcgc cgctgcgcag cagattcagc atatgctggg tgccggtcag gtgcagggct
    16741 tcgtcgcggg caatcaggcg aataattttg gcgttgcctt ccatcaattc gcgttctgca
    16801 aaggcaaagg aacaagcaaa gctgacgtag aagcggatcg cttccagcgc gttaacgctc
    16861 atcaggcaaa gatacagttt tttcttcagc tcgcgcaggc taacggttac ggttttaccg
    16921 ttaacggtgt gggtgccttc gcccagaagg tgccagtagc tggtcatttc gatcagttca
    16981 tcgtaatagc tggagatccc ttccgcacgt ttctggatct gctcgttggt gacgatatcg
    17041 tcaaacacaa cagacggatc gttaacgata ttacgaatga tatgagtata ggaacgggaa
    17101 tgaatcgttt ctgagaacgc ccaggtttcg acccaggttt ccagttccgg aatagaaata
    17161 agcggcaata gcgccacgtt cggactacga ccctgaatgg aatccagcag cgtctgatat
    17221 ttcaggttgc tgataaaaat gtgtttttcg tgctccggca gcgcctggta atcgatacgg
    17281 tcgcgggaga cgtcaacttc ttccggacgc cagaagaaag agagctgctt ttcgatcagc
    17341 ttttcgaaga tgtcatattt ttgctgatcg tagcgggcca cgttgaccgg ctgaccaaag
    17401 aacatcggtt ctttgagctg atcatttttc gtctgtgaaa aggtggtata tgccatgagt
    17461 gtgtcctgtt gggagtttaa tgccggataa ggcattttac gccgcatcct gtgcctgatg
    17521 cgacgctggc gcgtcttatc aggcctacaa atccgagccg taggccggat aaggcgttca
    17581 cgctgcatcc ggcatctcaa tatcagatct tacatgcgcc gctttcgcag ccatcgtcct
    17641 ggattgacgg taccagatcg tcctgcgcgt cttcagcgcc gtcacgggtg ttctgataat
    17701 acagcgtttt gaccccgaat ttgtaggcgg tgagcaggtc tttcagcaac tgctgcatcg
    17761 gcacttttcc tgacgggaaa cgtgacggat cgtagttggt gttagcagag atcgactgat
    17821 cgataaattt ctgcatgata cctaccagtt gcaggtagcc atcgttaccc ggcatttccc
    17881 acagcagctc ataggcgtcg tgcaggtgct cgtagtccgg caccacctgg cgcaaaatac
    17941 cgtctttcga cgctttgatg ctgacgtaac cgcgcggcgg ttcaataccg ttagtggcgt
    18001 tagagatctg cgaagaggtc tcggacggca tcagggcaga aagcgtggag ttacgcaggc
    18061 cgtgcgtttt gattgattca cgcaatgctt cccagtcgta atgcagcggc tcattagcga
    18121 tggcatccag atctttctta taggtatcga tcggcaggat ccctttcgcg taagtggttt
    18181 cgttaaacca cgggcactcg ccttgctctt tagccagctc attagacgct ttcagcaggt
    18241 aatactgaat cgcttcgaag gttttatgcg tcaggttgtt ggcgctgccg tcggagtagc
    18301 gtttaccgtg cttcgccagg tagtaagcga agttgatcac accaataccc agcgtacgac
    18361 gacccatcgc tccacgtttg gcggccggga tcgggtaatc ctgataatcc agcagcgcgt
    18421 caagtgcacg aaccgccaga attgccagct cttccagttc atccaggtta ttaattgcgc
    18481 ccaggttgaa agcagacagc gtacacagcg cgatttcacc gttctcgtcg ttgacgtcgt
    18541 tcagcggttt ggtcggcagg gctatctcca ggcacaggtt agactgacgc actggcgcga
    18601 tggccggatc aaacgggcta tgggtattac agtggtcaac gttctgaata tagatacgac
    18661 cggtagacgc acgttcctgc atcatcagcg agaacagctc aacggctttc acacgctgct
    18721 tgcggatgct gtcgtctttc tcatatttgg tatacagacg ttcaaactct tcctgatcgg
    18781 cgaagaacgc gtcgtacagc cccggtacgt cggacgggct gaacagggtg atatcttcac
    18841 ctttcagcag acgggtatac atcagtttgt tgatttgtac cccgtagtcc atatgacgca
    18901 cgcggttgcc ttccacacca cggttgtttt tcaacaccag caggctttcc acttccagat
    18961 gccacatcgg gtagaacagc gttgccgcac cgccgcgcac accgccctga gagcaggatt
    19021 tcaccgctgt ctggaaatgt ttgtagaacg gaatgcagcc ggtatggaac gcttcaccac
    19081 cgcgaatcgg gctacccagc gcacgaatac gcccggcgtt gatgccgatc ccggcacgct
    19141 gggaaacgta tttaacaatc gcgctggagg tggcgttgat ggaatccagg ctgtcaccgc
    19201 actcgatcag tacgcaggag ctgaactgac gagtcggggt acgcacgccg gacatgattg
    19261 gcgtcggcag cgaaatttta aatgtggaaa ccgcgtcgta aaaacgcttc acatattgca
    19321 ggcgcgtttc acgcgggtag ttcgagaaca ggcacgcggc aaccagaata taaaggaact
    19381 gggcgctctc atagatttcg ccggtcacgc ggttctgtac cagatatttg ccttccagct
    19441 gcttaacggc agcataagag aaggtcatat cacggtcgtg atcgataaac gtgtccatct
    19501 gcttgaactc ttcttccgtg tagtcttcca gcagatgatt atcgtatttg cccatctcga
    19561 ccattttcac cacgtggtcg tacagcgcag gcggctcaaa ctggccgtag gcttttttac
    19621 gcaggtggaa gatcgccagg cgcgcggcga gatactgata atccggcgca tcacgggaga
    19681 tcaggtctgc ggcagccttg ataatggttt cgtggatgtc agaggtcttg ataccgtcat
    19741 aaaactgaat gtgggagcgc agctcgacct gggaaatcga aacgttatgc agtccttctg
    19801 ccgcccaatc cagaacgcga tggattttgt cgagattgat gcgctctgtg ctaccgtcgc
    19861 gctttgtcac cagcagattc tgattcatgt atgtcgtacc tgtttttgga attctttccg
    19921 caatagtttc atgcagacta cctgtagtac gggaagctgc ttagaacact atatataggg
    19981 ggtatgtttg ggattcactg caagatagtg tgaaaatggc cctcttgcaa gtgcataact
    20041 ttgtggataa ctcaggaagg aaaaagtggc tttcgcgcac cttaggtcag acaaggtgtc
    20101 cgggaaagtc aatgggaaga gaaaaatttg ttaaaaataa ctgttcggcg atttcttgaa
    20161 cggtagaata ggttggataa attgatatag atggtttatt aatctttttg ctaatgattg
    20221 caaaaattga tctaagcacg agtcatcaca taatctgatg tatcagttgt ggtcaatata
    20281 taagcgaatg taagcgattg ttgatgtcga aatggttgtt ttctattcgg ttattgttaa
    20341 atgcgaagcc attttgttaa tttaccttat attataaaaa aattattcat tactttaatt
    20401 tagtttaaat ataactggta tcccttaagg ttattaactt gaattatgac gcactgatat
    20461 tattcatcaa ataataacaa aatagccatt gcatcgggtt ggacaattta caaaagaaag
    20521 ataacccata ttgtataatg cgtttaaata tgccgtctta tataaaatga tggtcagatt
    20581 aatatgcgga ttatctttct acg
//
LOCUS       DAJTMC010000066        20248 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.066, whole genome shotgun sequence.
ACCESSION   DAJTMC010000066 DAJTMC010000000
VERSION     DAJTMC010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20248
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.066"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(52..1074)
                     /locus_tag="OO130_002094"
                     /pseudo
     CDS             complement(52..1074)
                     /locus_tag="OO130_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
     gene            complement(1084..1896)
                     /gene="hcaB"
                     /locus_tag="OO130_002095"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             complement(1084..1896)
                     /gene="hcaB"
                     /locus_tag="OO130_002095"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HCR5365508.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            complement(1893..2213)
                     /locus_tag="OO130_002096"
     CDS             complement(1893..2213)
                     /locus_tag="OO130_002096"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HCR5365509.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGSDIFIDLP
                     EAQP"
     gene            complement(2213..2731)
                     /locus_tag="OO130_002097"
     CDS             complement(2213..2731)
                     /locus_tag="OO130_002097"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HCR5365510.1"
                     /translation="MSAQVSLELHLRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRPRHLISNC
                     QISETDIPNVFAVRVNYLLYQAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            complement(2728..4089)
                     /locus_tag="OO130_002098"
     CDS             complement(2728..4089)
                     /locus_tag="OO130_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HCR5365511.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKSPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            4225..5115
                     /gene="hcaR"
                     /locus_tag="OO130_002099"
     CDS             4225..5115
                     /gene="hcaR"
                     /locus_tag="OO130_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HCR5365512.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            5275..6414
                     /locus_tag="OO130_002100"
     CDS             5275..6414
                     /locus_tag="OO130_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HCR5365513.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLAMMTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYQVIL
                     ALLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVLQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(6406..7686)
                     /gene="csiE"
                     /locus_tag="OO130_002101"
     CDS             complement(6406..7686)
                     /gene="csiE"
                     /locus_tag="OO130_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HCR5365514.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFETRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(7877..8731)
                     /locus_tag="OO130_002102"
     CDS             complement(7877..8731)
                     /locus_tag="OO130_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR5365515.1"
                     /translation="MALPVNKRVLKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILAVIGQDDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKETKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            complement(8876..9679)
                     /gene="suhB"
                     /locus_tag="OO130_002103"
     CDS             complement(8876..9679)
                     /gene="suhB"
                     /locus_tag="OO130_002103"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HCR5365516.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAVEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            9798..10538
                     /gene="trmJ"
                     /locus_tag="OO130_002104"
     CDS             9798..10538
                     /gene="trmJ"
                     /locus_tag="OO130_002104"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HCR5365517.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            10808..11296
                     /gene="iscR"
                     /locus_tag="OO130_002105"
     CDS             10808..11296
                     /gene="iscR"
                     /locus_tag="OO130_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HCR5365518.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            11408..12622
                     /gene="iscS"
                     /locus_tag="OO130_002106"
     CDS             11408..12622
                     /gene="iscS"
                     /locus_tag="OO130_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCR5365519.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            12650..13036
                     /gene="iscU"
                     /locus_tag="OO130_002107"
     CDS             12650..13036
                     /gene="iscU"
                     /locus_tag="OO130_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HCR5365520.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLVEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            13053..13376
                     /gene="iscA"
                     /locus_tag="OO130_002108"
     CDS             13053..13376
                     /gene="iscA"
                     /locus_tag="OO130_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HCR5365521.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            13472..13987
                     /gene="hscB"
                     /locus_tag="OO130_002109"
     CDS             13472..13987
                     /gene="hscB"
                     /locus_tag="OO130_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HCR5365522.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNETWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            14004..15854
                     /gene="hscA"
                     /locus_tag="OO130_002110"
     CDS             14004..15854
                     /gene="hscA"
                     /locus_tag="OO130_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HCR5365523.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQADIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDNAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            15856..16191
                     /gene="fdx"
                     /locus_tag="OO130_002111"
     CDS             15856..16191
                     /gene="fdx"
                     /locus_tag="OO130_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HCR5365524.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            16203..16403
                     /gene="iscX"
                     /locus_tag="OO130_002112"
     CDS             16203..16403
                     /gene="iscX"
                     /locus_tag="OO130_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HCR5365525.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            16581..17864
                     /gene="pepB"
                     /locus_tag="OO130_002113"
     CDS             16581..17864
                     /gene="pepB"
                     /locus_tag="OO130_002113"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HCR5365526.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRASERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            18006..18782
                     /gene="sseB"
                     /locus_tag="OO130_002114"
     CDS             18006..18782
                     /gene="sseB"
                     /locus_tag="OO130_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HCR5365527.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLFIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIVPFYERRWGGFLRDFKQNRII"
     gene            complement(19275..20120)
                     /gene="sseA"
                     /locus_tag="OO130_002115"
     CDS             complement(19275..20120)
                     /gene="sseA"
                     /locus_tag="OO130_002115"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HCR5365528.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPKTFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPVK"
BASE COUNT         4825 a         5155 c         5512 g         4756 t
ORIGIN      
        1 tgatgccatt ctatttcagg aaggagtgtc cgttaaactc aggctacctc agtcatcgcc
       61 acgcatatcg ccaataaact gtaagttatc actgtactga tcgctccaga accacggcag
      121 tagcggtagc ggtagcccta acattgcggc agcggcaatt tgcgcctggt tattggcgtt
      181 ttcccagctt tcacagcggt gtagtgcacc attatcaaga cgagtgattg ccacatcgcc
      241 accggcaaag atcgcgggat cgcaggtgcg gcaagcctca tcaatgacaa tgccattggc
      301 agtatcaagg ttggcctcgc gagccagttg ctcgttggcg ctgataccaa taccgtaaat
      361 caccacatca gcctgaagcg tttccccact ttgcagcgtc agttctactt tttcaccatc
      421 gaccacatgt tcaatggcat tattgagcag aatgcgcaca ccagcctgct ggtggcgctg
      481 taaaagatag cgttgcacgg gcggtggtgc attacggccc atgacggttg ccgccagttc
      541 aatcactgtc accttacatc tgcgctgcgc tgcgctgcgc tgcgtggcgc tggcagccag
      601 ttccagacca atagttccgg caccgacaat cacgactgac cgttcgggct gcagaacttc
      661 tcgcagtctg gcggcatcac cggcatggcg tagggtaaag cagcgttctc ccagtgcatc
      721 aagcaacggc agcggtcgag ctgccgcgcc ggttgctata aaaagctgat cccagtgcca
      781 gctttcgccg ttggttaaca ctaactctcg tgtgtcgcga cccagcgttt tgatggttac
      841 accggaatgc agatgaacat tgttttcctg ccaccagtta gcgggtaaca cctgctgtaa
      901 ctgcggggaa tcttccagca acatggattt cgagagtgga ggtcgttcat aaggaagatg
      961 tcgctcatcg gaaaacagat gcagctcacc ggtgaaccct tgctggcgta gcgaggccgc
     1021 agccattgcc gccgcttgca cgccaccgac aataatgatc gttttttctt tcatcgtgct
     1081 tccttaaaga tccagcccag ccgctacgtg gcgaatgccg cgaatcgcta aacccgcatc
     1141 agcgttgatc atcacaccgc ttaatgcgcg attattgcgc cgcgatgcca acatcacata
     1201 cggccccgta aaatccgccg gttgcgggaa aaattgcagc ggtaaaatgg cggcaatttt
     1261 ctccggcgtc agagactgca ttatcgaggt ttcactttgc ccgagcgcct gtgggccgcg
     1321 caggtcgctg gccataccac acgggccgac gccattcacc cgcactttcg gtgccagttc
     1381 ataagccagt tggcgaataa gtccggttgc ggcatgttta ctggcggtgt acagcgggcc
     1441 accgccgcca ggataccagg cggcatttga cagtgtgaaa atcatgctgc cttcactggc
     1501 gattaacgcc ggagcgcagg cttttgcgcc cagcaggtaa ccgagtacgt tgacgttaaa
     1561 cagctcgtgg aagccggttt cgagcgtctc tgcgggagta ttaaccagtg aggcattgtg
     1621 atcccagatg cctgcattgc cgataaaaca atccagcttg ccggaacgag tcaggatctg
     1681 atcgaccgcg cgttgataat cggcataaca ggtcacgtta ccttccaccg ccagaatatg
     1741 ttcgccaaat cgctgacgca gactggcgac ttttgccgcc gacagttcca gcgtggcaac
     1801 ctgcgcgcct tcttcgataa atcgctcgac cagcgccagc cctaatcccg atccgccgcc
     1861 ggtaataaaa atggactcgt tatgcagatc gctcatggct gcgcctccgg taagtcgatg
     1921 aaaatgtcac taccttcaac gtgtactgga taagtggtga gcggatcggt ggcgggcagg
     1981 cataacgctt tccccgtttt caggcaaaaa ctggcggcgt gtagcgggca ctccaccgtg
     2041 gcgtcatctt ccagataccc ttctgacatc gacgcattac catggctgca acgatcgtta
     2101 atggcataaa actcgccgcc aacgttgaac agggcgatga cgggcgaggt atcgatccgg
     2161 agagcctcac cctccggcac atccgcaacg ggacacgcat aaattcgatt catcagaaca
     2221 gtacactcag gttatgggaa gtgattaccg cttgatccag gacgatatcc cgttccagca
     2281 agcgccagtt gtcatcttcc agacggcgaa ctttgtcgaa acgcgttcca acatagaatg
     2341 tttcatcgcg ctctttttgt gcctgataaa gcagataatt tacccgcaca gcaaatacgt
     2401 ttgggatgtc ggtttcgctt atctggcagt tgctgattaa gtgacggggg cgtgacggcg
     2461 gctcttctgc ccaggccatg cccgtttcca gacgggcgat tcgccgctcc agctggtctt
     2521 tggtgtcatt aaaaatccag gttgtcggtg gctggacgcc tttgcggcgg tcgcgtgttt
     2581 gcgcgttaac tgtggtgcgc atggtgtaac gaatctcttc gtcgagctgc gccagccagt
     2641 cacgaaattt ccagtcgtcc agtaagctgg cttcgtgaaa gagaaactgg ctaatgcgga
     2701 gatgtaactc tagtgaaact tgcgcactca tttcatcacc tcctgctggt aagcggcggt
     2761 tttatcgagc acttcctgcc agctttcgct actcagaaga tcggcccagc gttggtacat
     2821 tccacgagcg gcagtttctg aaaagatata gttagtaatg ccaggaatgc cgtcgtcgcg
     2881 gcgcttttcc tgaccaagcc ccatttccag acacagtttg ctgttgcggg cgcggtggcc
     2941 tttaagcaat ttctggattt cacaccagtt ctccgagtca tcctgctcga gaaaaccagc
     3001 aggaccaaaa gcacgagtgg cgctgttttc aaaagcggct ttaacttcat cggaggcggc
     3061 tttgtcagta atacagaacg cccacacttc aacttgatca gggccgcgcg gatgccagac
     3121 gcggagtgtg gcagtgccgt ttagccatga aagcgtgggg aatatattgt tatgacccgc
     3181 caggcgcagg gcgcgaactt cacctaaacg ttgttctgct tcggcatagg tttcgcggta
     3241 atagctggaa accgcgccat cgacccagac attagcatcc ggtttttcag taaagaagaa
     3301 accgctaccg tgaccgtcct gaccaaattg cagcgcatct ttggcggttt cccacaccgg
     3361 gcgggcggtt tgtccatcac cgagacgctt atcgctgcca tcatctttcg cccctaatac
     3421 ctgaacggca gaagcatggc tgaacagagc atgatattgg tcactggcga actgctctgc
     3481 cggggatttc cagttacagt tgatcaccca cttttgtacg ccgccgacaa tttcggtgcc
     3541 gccttcgcga cgatccagca tgccatccag ataccaggca atgtcaccaa ggtaatcacg
     3601 caggcccggt gcgctggtat cccagttgcc aaaaatcagc cctttataac tctccacaca
     3661 aggaacttcg tttagccccc agtgggattt acacaaccct tgtgggtagg cgcgaggttc
     3721 cagcggtaca tcgatcaact cgccgttaat gccataagac cagccgtgat acgggcaggt
     3781 aaaggcgcga gtgttgccgc aatctgcata actcacacgc atggcccggt ggcggcattg
     3841 gttgagaaac gccttgatgc tgccgtcttt ctgacgcact acgacaaccg catcttctcc
     3901 catgtaggtg ttaaagaaat caccgggttt tgggatctgg ctttcgtggg cgagaaatag
     3961 ccagcaacga ccgaaaatac gctcaagctc cagttggtaa atatccgggt cggtataaat
     4021 acgcggagtg acccgaccat tttgggtgtc aatcagttgg taaatgttca aatctgaggg
     4081 tgtggtcata ttttttaatc ctgaaagata agatgtattg accttctatt tttgtgccat
     4141 aaggatgttg ctggttggtt gctaatatgt gaaatagtta atttctacca gctgagacgt
     4201 tttttcgata acaaggggaa ggtgatggaa ctacggcatt tacgctattt cgtcgcagtg
     4261 gcgcaggcac tgaactttac ccgtgcggcg gaaaaactgc atacctcaca gccttcgtta
     4321 agcagccaga tccgcgatct tgaaaactgt gtcggtgttc cgttactggt gagggataag
     4381 cgcaaagtcg cgttgacggc ggcgggagaa tgttttctcc aggacgcgct ggcaatcctc
     4441 gaacaagcgg aaaatgccaa attacgggcg cggaaaattg ttcaggaaga cagacaatta
     4501 accattggct ttgtgccatc ggcggaagtg aatttactgc caaaagtatt accgatgttt
     4561 cgtctcagac agccagacac cttgattgag ctggtgagtt taatcaccac gcaacaggag
     4621 gaaaaaattc gccgtggtga actcgacgtc ggcttaatgc gccatcctgt ttatagcccg
     4681 gagattgatt atctggagct ttttgacgaa ccattagtgg ttgtgttacc ggttgatcac
     4741 cctttagcac atgaaaaaga gatcaccgcc gcgcaactgg atggcgtgaa tttcgtcagt
     4801 accgatccgg cgtattccgg ttcgcttgcg ccgatcgtta aagcgtggtt tgcgcaagaa
     4861 aacagccagc caaatatcgt ccaggtggca acgaatattc tggtgaccat gaatctggtg
     4921 ggcatggggc tgggcgtcac tttgataccc ggttatatga ataattttaa taccggacag
     4981 gttgtttttc gtcccattgc cggaaatgtg ccttccattg ctttactgat ggcgtggaag
     5041 aaaggggaga tgaagccggc attacgcgat ttcatcgcca ttgtgcagga acgtttggca
     5101 agcgtaacgg cataattacg cattattttt gcaacatgcg tcgcggatcg cagtttttta
     5161 gtgcttgtga actggcgtta cagacatcgg gaagcaggct cgaatattct tatttttcaa
     5221 cttgcaccaa cattcgatta accccagact aacgcctcct gacgggaggg actcatggtt
     5281 ttgcaatcca cgcgctggtt ggcgctcggc tatttcacat acttttttag ttacggcatt
     5341 tttctacctt tctggagcgt ctggcttaaa gggattggtt taacgccaga aaccatcggc
     5401 ctgttattgg gggcaggtct ggttgcccgt tttctcggga gtttgctcat cgcgccccgc
     5461 gtcagcgatc cttctcgcct gatttccgcc ttgcgcgtgc tggcaatgat gacacttctc
     5521 tttgctgtcg ccttctgggc gggggcgcac gtagcgtggc tgatgctggt gatgattggc
     5581 tttaacctct ttttctcacc gctggtaccg ttgaccgatg cactggcgaa tacgtggcaa
     5641 aagcagttcc cgcttgatta cggcaaagtg cgactgtggg gctcggtggc gtttgtcatt
     5701 ggctcggcgc tgacgggcaa actggtcact atgtttgatt atcaggtgat cctcgcgctg
     5761 ttaacattgg gcgtggcatc catgctgctc ggctttctca tccgtccgac gattcagcca
     5821 caaggggcaa gccgccagca ggagagcacc ggttggtcag cgtggttggc gctggttctt
     5881 cagaactggc gctttctggc ctgcgtttgt ttattgcagg gggcacatgc ggcctattac
     5941 ggttttagcg ccatttactg gcaggcagct ggctactcgg cctcggcggt ggggtatttg
     6001 tggtcgctgg gcgtggtggc ggaagtcatt atctttgcgc tgagtaataa acttttccgc
     6061 cgttgtagtg cacgcgatat gctgttgatc tcggcgattt gcggcgtagt gcgctggggc
     6121 attatgggag caactacggc gttgccgtgg ttgatagtgg tgcaaattct gcattgcggc
     6181 accttcacgg tctgccacct ggccgccatg cgctatattg ccgctcgcca gggtagcgaa
     6241 gtcatccgtt tacaggcggt ttactctgcc gtcgcgatgg gcggcagtat agctatcatg
     6301 accgttttcg ccggtttcct gtatcaatat ctgggccacg gcgtgttctg ggtaatggcg
     6361 ctggtggcgc ttccggcaat gtttttgcgc ccgaaagttg ttccctcatg ctgattcgag
     6421 cattttgcgg atctgctcct gttgatgtgt tgtcagtgtc aggtccgtat agatcagcgg
     6481 tggtgaaaag agcggtaacg gcatggtata aggcgcaata atcagtgccg cgccgcgcgg
     6541 agcgcctgtc tgcaaaaatg cctttaccga catatgttta atgttgagcg gcagtaacgt
     6601 tagttcgcgt aactgctgct caatctgcgc ttctcgctcg ctatcattcc cggtaagaag
     6661 aataatctgt ttttcatgca gatcgttttc ctgcattagc caggcaccga aaatcaccgc
     6721 gaccagacca ctttcctcat cagaaagatg gacgccgtat tcactttcaa atccggccag
     6781 cgccgcgcgg gtggtgcgca ccaggcgtgg gtacagtctg gcgaactctt ctggcagggt
     6841 attatcaata ccgatggcaa acaaactgcg atttaacgcc tgggcgaggt gggtataaag
     6901 ctgatcgcat aacccctgtt catcataaaa acgaacattt cccagctcac gaaaatggtt
     6961 taccagacgt ttgatagact gacgcaattg tctgtctcgc tgatgcgcat cacgcaatgg
     7021 atcgggaacc cgcaacatgg aaaaaagtaa tgccataaac agtggctcat caggcggtac
     7081 aggctggagc gcccgacgct gccagtggcg tccaatttcc tgcgctacct gaaattcaag
     7141 gcaggattcc gcccagcgac gttggagcgg attaaactga ggcgtaattc cagcgtggtg
     7201 ttgcagcaaa caatattgca gatacagaca caggaaatga atatcgcgcg tttcaaagcg
     7261 tttttgcagc cgtcgggagc agagattcac taacgcttgt agattggtat cgtcgtaaaa
     7321 gttacgcgca attccgcgcc gcttcagttc actcttcagg gcgggggtga attggctggt
     7381 aataaatgac ggacacagac ggaaaccacg tcgtagccag tgaaataaac acaaacgttg
     7441 gttaagcact gtaccttcaa cccggtagct accgtcataa ccagtcgtca gtgtgagttg
     7501 atgatagcgc aggatctccc gtcctgtttc gctgatatca agactggcaa tatcatcatc
     7561 cacaccatta agctcgctga aggttgccat ggtggcggtc aaccccggct gaaagagcgt
     7621 cagcaagatc tggcagcggc gctggggagc cgaaaggaca gatggtggag caagcgtagg
     7681 catcatctgt agatctccca taagcataat tgataagaat agctaaagtt tgctcaggta
     7741 aatggcgaag ggaatgctaa tcatcagaaa tgttggcaag cgtcacagaa tttttctcat
     7801 cttaaactgt tctgaccagt ggattagcat gtcttaaggt ggttttgtga atgattatcg
     7861 tgctagcgat catgggttag ttctgcggat tcaacgcgct aaggataaag tccaccatct
     7921 gttcgcgata aacatcgccg tgacgatcgg aaaaagcatc aatgtgttcg ccatccggga
     7981 ttaggatcaa ccgttttgtc tcttttgcca ggctatacaa cttttcgctg tgctgccatg
     8041 ggataacgtg atcagcttta ccgtgaatga gtaaaagcgg gatcgggctg acgctggcga
     8101 tataattttc gccgctgtaa ctctcatcaa gtaagtagcc actgccgggg atcatctgat
     8161 tggcgatggt tgcataagag gcaaatgtgg agtcgaggat caccgcacgt atgccttcac
     8221 gatcatcctg accaataaca gcaagaatat tcgccccgcc aatactctgc ccgaacagca
     8281 ccagccgttg tgggtttaca tcactgcgat ggcgcaccac attgatggca ctttgcgtat
     8341 cgtccagcaa tccggcctgg gacggcgtgc cttttgattt accaaacccg cgataatcaa
     8401 acataaaaac gttgaaatta cgctcgggta accaactgac cagcggccag tgggcggaca
     8461 tatttccggc attgccgtga gcatgaatga tggttgcgat ggcattgtca gcagggcccg
     8521 tcgaagaagg aataaaccag ccttgcagac gagtaccgtc cttagccgta aattcgacgg
     8581 attccgccga ccaggggtcg ggaccgtaaa ttttatcgtc aggataatag aagaagttga
     8641 tggcaacgcg cggcactaaa taaacgcaga aggcaacaac aaagagaata aacagaattt
     8701 tgagaacgcg tttgttcact ggcagcgcca taacctttcc ttgtgacttc tttatcatcc
     8761 attaatcggg gaaagatggt gggagagtga cgaaagaaag taaagcggtg aggaagtatt
     8821 tcggaatagc gcctgaaagg cgagggcggg tgagtgacat cacccgcctg agtcattaac
     8881 gcttcagagc gtcgcttaac tcatcacgca tgttcgccag catggctttc acaacacgcg
     8941 ggttaccagc aacgatgtta ccggtcagca tatagttatg accaccggtg aagtcgctga
     9001 cgatgccgcc cgcttcacga accagcagct cgcctgccgc gaaatcccac ggacgcagac
     9061 cgatttcaaa gaaaccgtca acacgacccg cagcgacgta agccagatcc agcgccgcag
     9121 aaccggtacg acggaagtct gcacattcgt tgaacagttt gccgacgatg ttgatgtagg
     9181 tagtggcgta ctgttttgct ttgaacggga agccggtcgc cagaatggta ccgtcgagat
     9241 cgcgagcggt gctaccgcgc agacggtagc cgttcagctg tgcgccctga ccgcgagtgg
     9301 cggtgaacag ttcgttacgc ataggatcgt ataccacagc aacttcggtg cggcctttga
     9361 tacgaacggc gatagatacc gcgaagtgcg gcagacgttt gataaagttg gtagtaccat
     9421 ccagtggatc gataacccat tgaacatcct gatcagtacc ttcaagttca ccgctttctt
     9481 cggtgatgat ggtgtgctgt gggtaagatt tacgaatcgt gtcgataatc accgcttcga
     9541 cagctttatc tacgttggtc acgaaatcgt tactgccttt ctggctcgct tctacagcgt
     9601 ccggggtttc atagtttttg gcaattaaat tacccgcctt gcgcgctgcg cgcacggcga
     9661 tgttcagcat cggatgcatc ggtctctctc actggatgtt aaagaacggg aaaacggcgc
     9721 agagtatagc agcgagatcg gcatctgtct tcgttttatg ataagatgcg tgaataatct
     9781 ttagatgaag aaagacaatg ctgcaaaata ttcgaattgt gctggtggag acgtcacaca
     9841 ccggcaatat gggttctgtt gcccgtgcca tgaaaacaat gggattaacc aatctgtggc
     9901 tggttaatcc actggtgaaa cccgactccc aggcgattgc cctggcagca ggggccagcg
     9961 atgtgattgg taatgctcac atcgtcgata cgctcgacga agcgttagcc ggttgtagct
    10021 tggtggtggg caccagcgca cgttcccgca cgctgccatg gccgatgctc gacccgcgcg
    10081 aatgcggcct gaaaagcgtc gctgaagcgg caaatacccc ggtggcgctg gtgtttggtc
    10141 gcgagcgcgt cggcttgacc aatgaagagt tgcagaaatg ccattatcat gtcgcgattg
    10201 cggctaaccc ggaatacagc tcgctgaacc tggcgatggc ggttcaggtt attgcctatg
    10261 aagtgcgcat ggcctggctg gcaactcagg aaaacggcga gcaggtcgag catgaagaga
    10321 cgccgtatcc gctggtcgat gatctggagc gtttttatgg tcatctggaa caaacgctgc
    10381 tggcaaccgg ttttatccgt gaaaaccatc cggggcaggt gatgaataaa ttgcgccgtc
    10441 tgtttacccg tgcgcgcccg gaaagccagg aattgaatat cctgcgcggg attctggctt
    10501 ctattgagca gcagaataaa ggtaacaagg ccgaataaca gtagttgcct gatgcgacgc
    10561 ttaacgcgtc ttatcaggcc tacaatgtac agaaccgtag gtcggataag gtgttcacac
    10621 cgcatccgac aacaggtaca aacgccacga taaaaaaatg gcactgaagg ttaaataccc
    10681 gactaaatca gtcaagtaaa tagttgacca atttactcgg gaatgtcaga cttgaccctg
    10741 ctattcaata cccccacttt tacaataaaa aaccccgggc aggggcgagt ttgaggtgaa
    10801 gtaagacatg agactgacat ctaaagggcg ctatgccgtg accgcaatgc ttgacgttgc
    10861 gctcaactct gaagcgggcc cggtaccgtt ggctgatatt tccgaacgtc agggcatttc
    10921 cctttcttat ctggaacaac tgttttcccg tctgcgtaaa aatggtctgg tttccagcgt
    10981 acgtggacca ggcggtggtt atctgttagg caaagatgcc agcagcatcg ccgttggcga
    11041 agtgattagc gccgttgacg aatctgtaga tgccacccgt tgtcagggta aaggcggctg
    11101 ccagggcggc gataaatgcc tgacccacgc gctgtggcgt gatttgagcg accgtctcac
    11161 cggttttctc aacaacatta ctttaggcga actggttaat aaccaggaag tgctggatgt
    11221 gtctggtcgt cagcatactc acgacgcgcc acgcacccgc acacaagacg cgatcgacgt
    11281 taagttacgc gcttaataaa aagaattcag aatcaggccg gagtgctaaa tactccgtaa
    11341 acacggtcgt acatccagcc ggtagcctga ttccttgcat tgagtgatgt acggagttta
    11401 tagagcaatg aaattaccga tttatctcga ctactccgca accacgccgg tggacccgcg
    11461 tgttgccgag aaaatgatgc agttcatgac gatggacgga acctttggta acccggcctc
    11521 tcgttctcac cgtttcggct ggcaggctga agaagcggta gatatcgccc gtaatcagat
    11581 tgccgatctg gtcggcgctg atccgcgtga aatcgtcttt acctctggtg caaccgaatc
    11641 tgacaacctg gcgatcaaag gtgcagccaa cttttatcag aaaaaaggca agcacatcat
    11701 cactagcaaa accgaacaca aagcggtact ggatacctgc cgtcagctgg agcgcgaagg
    11761 ttttgaagtc acctacctgg caccgcagcg taacggcatt atcgacctga aagaacttga
    11821 agcagcgatg cgtgacgaca ccatccttgt ttccatcatg cacgtaaata acgaaatcgg
    11881 cgtggtgcag gatatcgcgg ctatcggcga aatgtgccgt gctcgtggca ttatctatca
    11941 cgttgatgca acccagagcg tgggcaaact gcctatcgac ctgagccagt tgaaagttga
    12001 cctgatgtct ttctccggtc acaaaatcta tggcccgaaa ggtatcggtg cgctgtatgt
    12061 acgtcgtaaa ccgcgcgtac gcatcgaagc gcaaatgcac ggcggcggtc acgagcgcgg
    12121 tatgcgttcc ggcactctgc ctgttcacca gatcgtcgga atgggcgagg cctatcgcat
    12181 cgcaaaagaa gagatggcga ccgagatgga acgtctgcgc ggcctgcgta accgtctgtg
    12241 gaacggcatc aaagatatcg aagaagttta cctgaacggt gacctggaac acggtgcgcc
    12301 gaacattctc aacgtcagct tcaactacgt tgaaggtgag tcgctgatta tggcgctgaa
    12361 agacctcgca gtttcttcag gttccgcctg tacgtcagca agcctcgaac cgtcctacgt
    12421 gctgcgcgcg ctggggctga acgacgagct ggcacatagc tctatccgtt tctctttagg
    12481 tcgttttact actgaagaag agatcgacta caccatcgag ttagttcgta aatccatcgg
    12541 tcgtctgcgt gacctttctc cgctgtggga aatgtacaag cagggcgtgg atctgaacag
    12601 catcgaatgg gctcatcatt aatcggtatc ggaatcagga gaatttataa tggcttacag
    12661 cgaaaaagtt atcgaccatt acgagaatcc gcgtaacgtg ggttcctttg acaacaacga
    12721 cgagaacgtc ggcagcggca tggtgggtgc accggcctgt ggcgacgtga tgaagttgca
    12781 gattaaagtc aacgatgaag gtatcattga agacgcgcgt tttaaaactt acggctgcgg
    12841 ttctgctatc gcttccagct ccctggtcac cgaatgggtg aaagggaagt ctctcgtcga
    12901 agcgcaggcg atcaaaaaca ccgatattgc tgaagaactt gaactgccgc cggtgaaaat
    12961 tcactgttct attctggcag aagacgcgat caaagccgcc attgcggact ataaaagcaa
    13021 acgtgaagca aaataagagt tgaggtttgg ttatgtcgat tacactgagc gacagtgcag
    13081 cagcgcgagt aaataccttt ctggctaacc gcggcaaagg gtttggcctg cgtctgggcg
    13141 tgagaacctc cggatgttca ggtatggctt atgtactgga atttgttgac gaaccgacgc
    13201 cggaagacat cgtgtttgaa gacaaaggcg tgaaagtcgt ggtcgatggc aaaagcctgc
    13261 aatttctgga cggtacgcag ctggacttcg taaaagaagg cctgaatgaa gggtttaaat
    13321 tcaccaaccc gaacgttaaa gatgagtgtg gttgcggcga aagcttccac gtttgatgcg
    13381 cataccgata accccaccgt ggtcgcctgc gcgtggggtt tgttttacct gattcgccgt
    13441 tatcgcggcg gatcgcagcc ctgagaatgt tatggattac ttcaccctct ttggcttgcc
    13501 tgcccgttat caactcgata cccaggcact gagcctgcgt tttcaggatc tacaacgtca
    13561 gtatcatcct gataaattcg ccagcggaag ccaggcggaa caactcgccg ccgtacagca
    13621 atctgcaacc attaaccagg cctggcaaac gctgcgtcat ccgttaatgc gcgcggaata
    13681 tttgctttct ttgcacggtt ttgatctcgc cagcgagcag catactgtgc gcgacaccgc
    13741 gttcctgatg gaacagctgg agctgcgcga agaactggac gagatcgaac aggcgaaaga
    13801 tgaagcgcgg ctggaaagct ttatcaaacg tgtgaaaaag atgtttgata cccgccatca
    13861 gttgatggtt gaacagttag acaacgagac gtgggacgcg gcggctgata ccgtgcgtaa
    13921 gctgcgtttt ctcgataaac tgcgaagcag tgccgaacaa ctcgaagaaa aactgctcga
    13981 tttttaattt ctggaagcta aacatggcct tattacaaat tagtgaacct ggtttgagtg
    14041 ctgcgccgca tcagcgtcgt ctggcggccg gtattgacct gggcacaacc aactcgctgg
    14101 tagcgacagt gcgcagcggt caggccgaaa cgttagccga ccatgaaggc cgtcacctgc
    14161 tgccatctgt tgttcactat caacagcaag ggcattcggt gggttatgac gcgcgtacta
    14221 acgcagcgct cgataccgcc aacaccatta gttctgttaa acgcctgatg ggacgctcgc
    14281 tggctgatat ccagcaacgc tatccgcatt tgccttatca attccaggcc agcgaaaacg
    14341 gcctgccaat gattgaaacg gcggcggggc tgctgaaccc ggtgcgcgtt tctgcggaca
    14401 tcctcaaagc actggcggcg cgggcaactg aagccctggc aggcgagctg gatggtgtcg
    14461 ttatcaccgt tccggcgtac tttgacgatg cccagcgtca gggcaccaaa gacgcggcgc
    14521 gtctggcggg gttgcatgtt ctgcgcttac ttaacgaacc gaccgctgcg gctatcgcct
    14581 acgggctgga ttccggtcag gaaggcgtga tcgccgttta tgacctcggt ggcgggacgt
    14641 ttgatatttc cattctgcgc ttaagtcgcg gcgtgtttga agtgctggca accggcggtg
    14701 attccgcgct cggcggcgat gatttcgacc atctgctggc ggattacatt cgcgagcagg
    14761 cggacattcc tgatcgtagc gataaccgcg ttcagcgtga actgctggat gccgccattg
    14821 cagccaaaat cgcgctgagc gatgcggact ccgtgaccgt taacgttgcg ggctggcagg
    14881 gcgaaatcag ccgtgaacaa ttcaatgaac tgatcgcgcc actggtaaaa cgaaccttac
    14941 tggcttgtcg tcgtgcgctg aaagacgcgg gcgtagaagc tgatgaagtg ctggaagtgg
    15001 tgatggttgg cggttctact cgcgtgccgc tggtgcgtga acgggtaggc gaatttttcg
    15061 gtcgtccacc gctgacttcc atcgacccgg ataaagtcgt cgctattggc gcggcgattc
    15121 aggcggatat tctggtgggt aacaagccgg acagcgaaat gctgctgctt gatgtgatcc
    15181 cactgtcgct gggcctcgaa acgatgggcg gcctggtgga gaaagtgatt ccgcgtaata
    15241 ccactattcc ggtggcccgc gctcaggatt tcaccacctt taaagatggt cagacggcga
    15301 tgtctatcca tgtaatgcag ggtgagcgcg aactggtgca ggactgccgc tcactggcgc
    15361 gttttgcgct gcgtggtatt ccggcgctac cggctggcgg tgcgcatatt cgcgtgacgt
    15421 tccaggtcga tgccgacggt cttttgagcg tgacggcgat ggagaaatcc accggcgttg
    15481 aggcgtctat tcaggtcaaa ccttcttacg gtctgaccga tagcgaaatc gcttcgatga
    15541 tcaaagattc aatgagctat gccgagcagg acgtaaaagc ccgtatgctg gcagaacaaa
    15601 aagtagaagc ggcgcgtgtg ctggaaagtc tgcacggcgc gctggctgct gatgccgcgc
    15661 tgttaagcgc cgcagagcgt caggtcattg acaatgctgc cgctcacctg agtgaagtgg
    15721 ctcagggcga tgatgttgac gccatcgaac aagcgattaa aaacgtagac aaacaaaccc
    15781 aggatttcgc cgctcgccgc atggaccagt cggttcgtcg tgcgctgaaa ggccattccg
    15841 tggacgaggt ttaatatgcc aaagattgtt attttgcctc atcaggacct ctgccctgat
    15901 ggcgctgttc tggaagctaa tagcggtgaa accattctcg acgcagcgct gcgtaacggt
    15961 atcgagattg aacacgcctg tgaaaaatcc tgtgcttgca ccacctgcca ctgcatcgtt
    16021 cgtgaaggtt ttgactcgct gccagaaagc tcagagcagg aagacgacat gctggacaaa
    16081 gcctggggac tggagcccga aagccgttta agctgccagg cacgcgttac cgacgaagat
    16141 ttagtagtcg aaatcccgcg ttacactatc aaccatgcgc gtgagcatta acagaggtta
    16201 gtatgggact taagtggacc gatagccgcg aaattggcga agcactgtac gatgcgtatc
    16261 ctgatcttga tccgaaaacg gttcgattca ccgatatgca tcagtggatt tgcgatctgg
    16321 aagatttcga cgacgacccg caggcatcca acgagaaaat cctcgaagcg attttgttag
    16381 tctggctgga cgaggccgaa taatttccgc gtcatgcttc acgccgcaga tgcgttggct
    16441 gcgctcgttc accccagtca catagttatc tatgctcctg gggcttcact cacttgccgc
    16501 cttcctgcaa cgcgaatcat ttagcggaaa aatcctgggg ctgccaactg gcggcccttt
    16561 tacaaagaag gataactaaa atgacagaag cgatgaagat taccctctct acccaacctg
    16621 ccgacgcgcg ctggggagaa aaagcaactt acagcattaa taatgacggc attaccctgc
    16681 atttgaacgg ggcagacgat ctggggctga tccagcgtgc ggcgcgcaag attgacggtc
    16741 tgggcatcaa gcatgttcag ttaagcggtg aaggctggga tgcggatcgc tgctgggcat
    16801 tctggcaagg ttacaaagcc ccgaaaggca cgcgtaaagt ggagtggccg gatctggacg
    16861 atgcccagcg ccaggaactg gataaccgcc tgatgatcat cgactgggtg cgtgacacca
    16921 tcaacgcacc ggcagaagaa ttgggaccat cgcaactggc acagcgtgct gttgatctga
    16981 tcagcaacgt cgcgggcgat cgtgtgactt atcggatcac caaaggcgaa gatctgcgtg
    17041 agcaaggtta tatggggctg cacaccgtcg gacgcgcttc agaacgttct ccggtattgc
    17101 tggcgctgga ttacaaccca actggcgata aagaagcgcc agtgtacgcg tgcctggtag
    17161 gtaaaggtat cacttttgac tccggcggct acagcatcaa acagactgcg tttatggact
    17221 cgatgaagtc ggacatgggc ggcgcggcaa cggttaccgg ggcgctggca tttgccatta
    17281 cgcgcggact gaacaagcgc gtgaagctgt tcctctgctg tgcggataac ctgattagcg
    17341 gcaatgcgtt caagctgggc gatatcatca cctatcgcaa cggtaaaaaa gttgaagtga
    17401 tgaacactga tgccgaaggg cgtctggtgc ttgccgatgg tctgattgat gccagtgcgc
    17461 agaaaccgga aatgatcatt gatgcggcga ccctcaccgg ggcggcgaaa actgcgctgg
    17521 gtaatgatta tcacgcgctg ttcagttttg acgatgcgct tgccggtcgt ttgctggcga
    17581 gtgcctcaca agagaacgaa ccattctggc gtctgccgct ggcggaattc caccgcagcc
    17641 agctgccgtc taactttgcc gaactgaaca ataccggaag cgcggcgtat ccggcaggcg
    17701 cgagcacggc ggcgggcttc ctgtcgcact ttgttgagaa ctatcagcaa ggctggctgc
    17761 atatcgactg ctcggcgact taccgtaaag cgccggttga acagtggtct gcgggcgcta
    17821 cgggacttgg tgtgcgcacg atagctaatc tgttaacggc gtaaactaat atgccggatg
    17881 cggcgtaaac gccttatccg gcctacaaat tctgcaaatt caacaaattg cgaatccctt
    17941 gtaggcctga taagcgtagc gcatcaggca tttgcgcccc gaacgttgaa tgaattgtgg
    18001 ttcttatgtc cgaaacaaaa aacgaacttg aagacctgct ggaaaaagca gcaaccgaac
    18061 cggcacaccg cccggccttt ttccgtactc tactggaatc caccgtctgg gtgcctggta
    18121 cggcggcgca gggcgaggct gtggttgaag atagtgcgct tgatttacag cactgggaaa
    18181 aagaagacgg caccagcgtc attccttttt tcacctcgtt agaagcgctt caacaggcgg
    18241 ttgaagacga acaggcattt gtcgtaatgc ccgttcgcac attgtttgag atgacccttg
    18301 gcgaaacgct cttccttaat gccaaactgc cgaccggtaa agaatttatg ccgcgtgaaa
    18361 tcagtttgtt gattggtgaa gagggaaatc cgctgagcag ccaggaaatc ctggaaggcg
    18421 gtgaatcgct gatattatcg gaagttgcag agccgccagc acaaatgatt gattcactca
    18481 ccaccttatt taaaaccatt aagccggtga agcgtgcttt tatttgttca attaaagaga
    18541 acgaagaggc acagcctaat ttatttattg gcattgaagc cgatggtgat atcgaagaaa
    18601 ttattcaggc gacgggaagt gtagcgaccg atacattacc tggcgatgaa ccaatcgata
    18661 tttgtcaggt gaaaaaaggg gaaaaaggaa ttagccactt tattaccgaa catattgtgc
    18721 cattctatga acgtcgctgg ggaggttttt tgcgtgactt taaacaaaac cggataatct
    18781 aaaatatgcc gccctaaagg gcggcattcg ggcttacttg ctgctctgaa ggttgttaac
    18841 caccagtaaa ttgtcgccgg agagactccg gttattattt acttgggaaa acagcgcacc
    18901 agaaacaata agtacgatac agaagcaccg tatcttcatt gtgcggtcca tgcgaagacc
    18961 gcatcacgac agcaccactg gcgatattgc cgttagagca agcgctgaga cgcgcttgct
    19021 ctaacggtcg cggaacatcc tttccttaag ccagcgcccg cttgcgagac ggcggaaagg
    19081 gccgcagtgg ataatatact aacagaacaa taataactct cttttgcttg acaaaaagag
    19141 agttactggc gagtattgtc tccgctgaga cgcgcttgca ttactacggt aatacaacct
    19201 gctggaaagg ccgcgaacca gaccagcaat aaaaaaccgc caaattcggc ggttttttat
    19261 tgcctgtaaa atacttattt cactggctca accggtaaat ctgcccgcgc gccccattca
    19321 ctccatgcgc cgtcgtacag tttcacgttt ggcacatcca gcgtcgcgag tgctaacaaa
    19381 accacggctg ccgttacacc agagccgcag ctgacgataa ttggtttgtc gtagctgacg
    19441 ccgcgaccaa aaaatatcgc atccagttca tcggtcgttt ttagttcgcc ttcgcgcacc
    19501 agttccgtcc acggcacatt cagtgctccg ggaatatgtc cgcgacgtaa acctgggcga
    19561 ggttcatcaa cttctgcgtt aaaacgtgca gccgggcggg catcaataat ttgcgccgta
    19621 ttttcatggc ttgccaataa tacatcggtt actttcacca cggcttcagg attaaacgcg
    19681 gcgttaaact ctccttccgg cagctctact gcaccttctt ctaacagcag atcatcgcgc
    19741 tgccagcctg caagtccacc ccccagaatc gacactttct ctacaccaaa ggtgcgcagc
    19801 atccaccatg ctcgtggggc tgagaaaaga ttaccttcgt catagacaat caggtgctta
    19861 tcctggttaa cgcctaattc acgcatcgcc acggcgaacg ttttcgggcg cggcagcatg
    19921 tgcggaagcg gggaagtgtg atcagaaagc gcttcgatat caaaaaacac tgcgccggga
    19981 atatgtccat tcagatactc ctgagcaacg ttacgatctt cctgtccagg cgacgccatg
    20041 cgggcatcga taatctgaat ttccgggtca tcaatatgtt cggcgagcca gtcggctcct
    20101 acaaaccatg tcgtggacat aggcatctcc atttttgccg gggttaggct aaattgtcgg
    20161 atgtttagtg aaaagcgaca agcgaaagag gctgatttgg ctgtctgagc cacaattctc
    20221 actaaaattc ggattgtatg ggcgtgat
//
LOCUS       DAJTMC010000067        19875 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.067, whole genome shotgun sequence.
ACCESSION   DAJTMC010000067 DAJTMC010000000
VERSION     DAJTMC010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19875
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.067"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            54..830
                     /gene="eutC"
                     /locus_tag="OO130_002116"
     CDS             54..830
                     /gene="eutC"
                     /locus_tag="OO130_002116"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutC"
                     /protein_id="HCR5365529.1"
                     /translation="MTSESCALDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTG
                     RAGPRPRTQALLRFLADHSRSKDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDM
                     GRRLCAEAVEALKAQCVANPDVQVVISDGLSTDAITVNYEELLPPLMAGLKQAGLKVG
                     TPFFVRYGRVKIEDQICEILGAKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEAD
                     RTCISNIHQGGTPPVEAAAVIVDLAKRMLEQKASGINMTR"
     gene            840..1499
                     /gene="eutL"
                     /locus_tag="OO130_002117"
     CDS             840..1499
                     /gene="eutL"
                     /locus_tag="OO130_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HCR5365530.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            1512..2012
                     /gene="eutK"
                     /locus_tag="OO130_002118"
     CDS             1512..2012
                     /gene="eutK"
                     /locus_tag="OO130_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HCR5365531.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAAGRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVREPD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            2058..3110
                     /gene="eutR"
                     /locus_tag="OO130_002119"
     CDS             2058..3110
                     /gene="eutR"
                     /locus_tag="OO130_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HCR5365532.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEDAQPMVTAESISHQSYRRLLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFVTDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(3116..4015)
                     /gene="hemF"
                     /locus_tag="OO130_002120"
     CDS             complement(3116..4015)
                     /gene="hemF"
                     /locus_tag="OO130_002120"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HCR5365533.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCNDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(4019..4888)
                     /gene="amiA"
                     /locus_tag="OO130_002121"
     CDS             complement(4019..4888)
                     /gene="amiA"
                     /locus_tag="OO130_002121"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HCR5365534.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            5102..5527
                     /locus_tag="OO130_002122"
     CDS             5102..5527
                     /locus_tag="OO130_002122"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HCR5365535.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADMLSLGKRLIEDEEY"
     gene            5514..5963
                     /locus_tag="OO130_002123"
     CDS             5514..5963
                     /locus_tag="OO130_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HCR5365536.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFIIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRASFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            6170..6745
                     /locus_tag="OO130_002124"
     CDS             6170..6745
                     /locus_tag="OO130_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HCR5365537.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFRGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            6841..7740
                     /gene="yfeX"
                     /locus_tag="OO130_002125"
     CDS             6841..7740
                     /gene="yfeX"
                     /locus_tag="OO130_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HCR5365538.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(7798..9102)
                     /gene="pbp4b"
                     /locus_tag="OO130_002126"
                     /pseudo
     CDS             complement(7798..9102)
                     /gene="pbp4b"
                     /locus_tag="OO130_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311328.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
     gene            complement(9107..10531)
                     /gene="murP"
                     /locus_tag="OO130_002127"
     CDS             complement(9107..10531)
                     /gene="murP"
                     /locus_tag="OO130_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HCR5365539.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKSVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRSQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFVTACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            complement(10535..11431)
                     /gene="murQ"
                     /locus_tag="OO130_002128"
     CDS             complement(10535..11431)
                     /gene="murQ"
                     /locus_tag="OO130_002128"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HCR5365540.1"
                     /translation="MQLEKMITEGSNAASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            11595..12452
                     /gene="murR"
                     /locus_tag="OO130_002129"
     CDS             11595..12452
                     /gene="murR"
                     /locus_tag="OO130_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HCR5365541.1"
                     /translation="MLYLTKISNAGSEFTENEQKIADFLQANVSELQSVSSRQMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNTTALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCVEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVASLKMIQR
                     SSELTQRLK"
     gene            12581..13372
                     /gene="ucpA"
                     /locus_tag="OO130_002130"
     CDS             12581..13372
                     /gene="ucpA"
                     /locus_tag="OO130_002130"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HCR5365542.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DDDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPM
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            13676..14692
                     /gene="cysP"
                     /locus_tag="OO130_002131"
     CDS             13676..14692
                     /gene="cysP"
                     /locus_tag="OO130_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HCR5365543.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKTDVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRN"
     gene            14692..15525
                     /gene="cysT"
                     /locus_tag="OO130_002132"
     CDS             14692..15525
                     /gene="cysT"
                     /locus_tag="OO130_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HCR5365544.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            15525..16400
                     /gene="cysW"
                     /locus_tag="OO130_002133"
     CDS             15525..16400
                     /gene="cysW"
                     /locus_tag="OO130_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HCR5365545.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            16390..17487
                     /gene="cysA"
                     /locus_tag="OO130_002134"
     CDS             16390..17487
                     /gene="cysA"
                     /locus_tag="OO130_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HCR5365546.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPDQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            17621..18532
                     /gene="cysM"
                     /locus_tag="OO130_002135"
     CDS             17621..18532
                     /gene="cysM"
                     /locus_tag="OO130_002135"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HCR5365547.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPNNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(18535..18903)
                     /locus_tag="OO130_002136"
     CDS             complement(18535..18903)
                     /locus_tag="OO130_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HCR5365548.1"
                     /translation="MKKIICLVITLLMTLPAYAKLTAHEEARINAMLEGLSQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            19008..19859
                     /gene="pdxK"
                     /locus_tag="OO130_002137"
                     /gene_synonym="thiJ"
     CDS             19008..19859
                     /gene="pdxK"
                     /locus_tag="OO130_002137"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HCR5365549.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
BASE COUNT         4571 a         5044 c         5610 g         4650 t
ORIGIN      
        1 cggcccccgc gctgtcagaa gcaaagtgca ccaccaccac ctgtgcggca ccggtgacct
       61 cggaaagctg cgcgctggat ttaggttctg cggaagcaaa agcgtggatt ggtgttgaaa
      121 acccgcatcg cgcagacgta ttaacagaac tgcgtcgcag caccgtggcc cgcgtttgta
      181 ccggtcgtgc cggtccgcgt ccgcgtaccc aggcgttgct gcgtttcctg gccgatcact
      241 ctcgttcgaa agataccgta ctgaaagaag tgccggaaga gtgggtgaaa gcgcagggct
      301 tgctggaagt acgctccgaa attagcgaca aaaatctcta cctgactcgc ccggatatgg
      361 gccgccgcct gtgtgcagaa gctgttgaag cgctgaaagc acagtgtgtt gctaatccgg
      421 acgtacaggt cgttatttct gatggcctgt caactgatgc catcaccgtg aactacgaag
      481 agctcctgcc gccgctgatg gcgggcctga aacaggccgg gctgaaagtc ggcacgccat
      541 tctttgttcg ttatggtcgc gtgaagattg aagatcagat ctgtgagatc ctcggcgcga
      601 aagtggtgat cctgctggtg ggggaacgtc cggggttggg tcagtcagaa agcctctcct
      661 gttacgccgt ttactcgccg cgtatggcga ccaccgttga ggccgatcgc acctgcatct
      721 ctaacattca ccagggcggc acgccgccgg ttgaagccgc agccgtcatt gtggatttgg
      781 cgaaacgtat gctggagcag aaagcatccg gcatcaacat gacccgataa ggaggcatca
      841 tgccagcttt agatttgatt cgaccgtcgg taaccgccat gcgggtgatt gcctccgtta
      901 acgctgaatt tgcgcgtgaa ctaaaattgc cgccgcatat tcgtagcctc gggctgattt
      961 ctgctgattc cgatgacgtc acctacattg cggctgacga agcgaccaag caggcgatgg
     1021 tcgaagtggt atatggccgc tcgctgtatg cgggagcagc acacggaccg tcaccgactg
     1081 ccggtgaagt gctgattatg cttggtgggc caaacccggc ggaagtgcgt gctggtctgg
     1141 atgcgatggt tgcgcatatt gaaaatggcg ctgctttcca gtgggctaat gacgcagaaa
     1201 acaccgcatt cctggcacat gtagtttcgc gtaccggttc ttatctctca tcaaccgccg
     1261 ggatcacgct tggcgatccg atggcgtatc tggtggcacc gccgctggaa gcgacctacg
     1321 gcattgatgc agcgttgaaa tctgccgacg tacagctggt gacctatgtc ccgccaccgt
     1381 ctgaaaccaa ctactcggca gcatttttaa ccggtagcca ggccgcgtgt aaagcagcct
     1441 gtaacgcctt taccgatgca gtgctggaaa tcgcgcgtaa tccaatccag cgtgcgtaac
     1501 ggaggttgcc gatgatcaat gcactgggat tgctggaagt ggacggcatg gtcgccgcga
     1561 tagatgcagc ggatgccatg ctcaaagcag ctaacgttcg tctgctcagt cacgaagtgc
     1621 ttgaccctgg tcgcttaacg ctggtggtgg aaggcgatct ggcggcgggt cgtgcggcgc
     1681 tggacgcagg ttgtgctgcc gcgatgcgta ccgggcgtgt catcagccgc aaggagatcg
     1741 gtcggccaga cgatgacacc cagtggctgg ttactggctt taaccgccag ccgaagcaac
     1801 ccgtaaggga acccgacgcg ccagttatcg tcgcggaatc tgctgacgag ttgttggcgc
     1861 tgttaacatc agtacgtcag ggaatgacgg caggagaagt ggctgcccac tttggctggc
     1921 cgctggaaaa agccagaaat gcgctcgaac agctcttttc tgccgggacg ttacgtaaac
     1981 gcagtagtcg ttatcgtctc aagccccatt aacctgtcgg aggtgctggg tgtcctacaa
     2041 cacccggcat taacatcatg aaaaagaccc gtacagccaa tttgcaccat ctttatcatg
     2101 aacccttacc cgaaaacctg aagctcacgc cgaaggtcga agtggataat gttcatcaac
     2161 gacagacaac ggatgtctat gaacatgctt taacgattac cgcctggcag cagatttacg
     2221 atcagctgca tccgggcaag tttcatggtg aatttacgga aattctactc gatgatattc
     2281 aggtttttcg tgaatacacc ggtctggcgc tgcgtcagtc gtgcctggtc tggccgaact
     2341 cgttctggtt tggcattccg gcgacgcgcg gtgagcaggg atttatcggt tcgcaatgtc
     2401 tgggaagcgc ggaaatcgcc acccgccctg gtggcactga atttgaactg agcacgccgg
     2461 atgattacac gatcctgggc gtggtgcttt ctgaagatgt catcacccgg caggctaact
     2521 ttttgcataa cccggatcgg gtattacata tgctgcgtag ccagtcggcg ctggaagtga
     2581 aagagcagca taaagccgcg ctgtggggct ttgtccaaca ggcgctggcg acgttttgcg
     2641 agaacccgga aaatctccat cagcctgcag tgcgaaaagt gctgggggat aatttgctaa
     2701 tggcgatggg ggcgatgctg gaagacgcgc aaccaatggt gacggcggaa agcatcagtc
     2761 atcagagtta ccgtagatta ctttcccgcg cccgtgaata tgtgctggaa aatatgtctg
     2821 agccggtgac ggtgctggac ttgtgtaatc aactgcatgt cagtcgccgc acgctacaaa
     2881 acgcgtttca cgctatttta ggcattggcc caaacgcgtg gctgaaacgc attcgcctga
     2941 acgccgtacg ccgcgaactg ataagcccgt ggtcgcaaag cacaacggta aaagacgccg
     3001 ccatgcagtg gggattctgg catctggggc aatttgtcac ggattaccag cagctgtttg
     3061 ccgagaagcc gtcgttgacg ttgcatcagc ggatgcggga atgggggtga gggagttaca
     3121 cccaatccct gaccttaata aactcactta acgccgcttc tgggctgcca tcttttggct
     3181 gataatcata ctcccagcgt accagtggcg gcattgacat caggatcgac tcggtgcgcc
     3241 cgccagtttg caggccaaac agagtgccgc gatcccagac cagattgaac tcgacataac
     3301 gaccgcgacg gtagagctga aaattgcgct cgcgctcgcc gtaggccatt gctttacgtc
     3361 gctctacaat tggtaaataa gcgtcggtgt agcctttgcc taccgcctgc ataaaggcaa
     3421 aacagtggtc gaaatctggc gtgttcagat catcaaagaa cagcccgcca ataccgcgct
     3481 gttcgttgcg atgtttgagg tagaagtaat cgttgcacca ctttttgtaa cggggataaa
     3541 cgtcttcacc aaatggcagg cacaggtcac gggcggtgcg gtgccagtga atggcgtctt
     3601 cttcaaaacc atagaaaggg gttaaatcga agccgccgcc aaaccaccag acgggctctg
     3661 cacccggttt ttcggcaata aaaaaacgca cattcgcgtg gctggtggga acatacgggt
     3721 tatgcggatg cactaccagt gaaacgccca tcgcctcgaa actgcgcccg gcaagttccg
     3781 ggcgatgagc ggtggcggaa gcaggcatcg cctcgccgtg gacgtgagaa aagttgacgc
     3841 ctgcctgttc gaaaacacca ccattacgca acacccgact acgcccgccg ccgccagctt
     3901 cgcgctgcca actatcttcg acaaattctg cgccatcgac ggcggtcagc tgctgacaaa
     3961 tcgtatcctg aaggttgagc agaaactgtt taacctggtg tgcgtcgggt ttcataactt
     4021 atcgcttttt cgaatgtgct ttctggttgt cgaaccagtg gaaataactg atcacgcctt
     4081 cagcaatcgc tgtggcgatt ttctgacgaa acgccgccgt gcctaacagc cgctcttctt
     4141 ccgggttggt gataaacgag gtttccacca gcaccgaagg aaccgacggc gatttcaaca
     4201 ccacaaacgc ggcttgttcg gtgttgcggc tgtgcagttt atgcaccggc ttaatctttt
     4261 tcagaatatg cgagccaagc gtcaggctgt ttttaatagt atcggtttgc accagatcaa
     4321 acagcacttg ctgcaatagg tgatccttgt cagtcgcctt tttaccggca acttcatcgg
     4381 cgcggttttc gcgttcagac aggtatttcg ccattgcgct acttgcccca cggttagaga
     4441 gggcaaatac cgaagcaccg gcagctttcg ggttggtaaa gccatcggca tgaattgaca
     4501 taaacagatc tgcgccatgt ttatgggcga tttcaacgcg atcgtaaagt gggataaacg
     4561 tatcgccaga acgcgttaaa cgcgcatcaa tcccatgatt acgcaaaatg gaacggacgt
     4621 ttttagcaat cgccagcacc acatgttttt ctttcgaacc gttgcgtccg atcgctccgg
     4681 tatcaattcc gccgtggcct ggatcgagaa caacgacacg tttgccgcca gattttttgg
     4741 ctttcggctt gctgtgtccg ttgctggttt ttaaaggttc gtctttggcg atggcttgcg
     4801 acattcctga caacgtcagg gcagccaaac cggctttcag cacctggcgg cgcgaagtga
     4861 gtgtttttag tggtttaaaa gtgctcatac ggcctgagtt gtaataataa agttccagat
     4921 gttatatcgt atcgcgtaat ccgttacgac ggtttgatgt tgagaattgt tttacttttc
     4981 atttcaatag ttgacaactc cccattatgc cactttttca accgattttc gtcagatatt
     5041 ggctaattct accgttcacg ccataatcag ccttttaacc cgcaaaaagg taacgaatac
     5101 catggagata cgcgtatttc gccaggaaga tttcgaagag gtcatcaccc tttgggagcg
     5161 ttgcgacttg ctgcgtccgt ggaacgatcc ggaaatggac atcgagcgta agatgaacca
     5221 tgacgtcagt ttgtttttgg tcgctgaggt gaacggtgaa gtggtcggaa cggtgatggg
     5281 cggttatgac gggcatcgcg ggtctgctta ttatcttggc gtgcatccag agtttcgtgg
     5341 gcgtgggatt gccaatgcgt tgcttaatcg gctggagaaa aagctgattg ctcgtggctg
     5401 cccgaaaatt cagatcaatg tgccggaaga taacgacatg gtgctcggaa tgtatgaacg
     5461 cctgggatat gaacacgccg acatgctgag tctgggtaag cgtttgattg aagatgaaga
     5521 gtactgagtt ccatcctgtc cattatgatg cgcacggacg cctgcgttta cccttgctct
     5581 tctggcttgt gctattgctt caggcgcgaa cctgggtgct gtttatcatt gccggtgcgt
     5641 cgcgcgagca gggtactgcg ctgctaaatc tgttttatcc cgatcacgat aatttctggc
     5701 tggggttgat tcccggtatt ccggcggtgc tggcgttttt gttgagcggt cggcgggctt
     5761 cgtttcctcg cacctggcat gtgctctatt ttctgttgct gttggcgcag gtggttttac
     5821 tttgctggca accgtggctg tggctgaacg gtgaatccgt tagcggtatc ggtctggcgc
     5881 tggtagtggc ggatattgtg gcgttaatct ggctgctgac caatcgacgt ttacgcgctt
     5941 gttttaatga agaaaaagaa taacgcagag gtgcgctttg tttatgccgg atgcggcgtg
     6001 aacgccttat ccggcctaca aaacatcgaa aattcaatat attgcaggag aaagtaggcc
     6061 tgataagcgt agcgcatcag gcaattttgt gtttgtcatc aacctttaac ggcacttttt
     6121 ggtgaatttg cactccaagc aacgttattg aataaccaaa ggaagtgaca tgaaatcgct
     6181 gcgtttaatg ttatgcgcta tgccgttgat gctgaccggc tgttccacga tgtcgtcagt
     6241 taactggtcg gctgctaacc cgtggaactg gtttggatcg tccaccaaag tgagcgagca
     6301 gggcgtgggt gaattaacgg cgtccacacc actgcaagaa caagccattg ctgatgcgct
     6361 tgatggcgat tatcgcctgc gcagcggaat gaaaaccgcg aacggcaacg tggtgcgctt
     6421 ttttgaagtg atgaaaggcg acaacgtggc gatggtgatt aacggcgatc aaggcacgat
     6481 cagtcgcatt gacgtgctgg atagcgatat tcctgctgac accggtgtta aaatcggtac
     6541 accgtttagc gacctttaca gcaaagcatt tggcaattgc caaaaagctg atggtgatga
     6601 taatcgtgcc gtcgaatgta aagccgaagg cagtcaacat attagctacc agttcagagg
     6661 ggaatggcgt ggtcctgaag ggttaatgcc ttcggacgat accctgaaaa actggaaagt
     6721 cagtaaaatt atctggcggc gttaatttgc gtctgaacaa accgccgccg cgaaaaaaca
     6781 cgtaaaatag cgctcaacaa tgccacggat tgcgtggcat tcttattttc aggaggaaca
     6841 atgtctcagg ttcagagtgg cattttgcca gaacattgcc gcgcggcgat ttggatcgaa
     6901 gccaacgtga aaggggaagt tgacgccctg cgtgcggcca gtaaaacatt tgccgacaaa
     6961 ctggcaactt ttgaagcgaa attcccggac gcgcatcttg gtgcggtggt tgcctttggt
     7021 aacaacacct ggcgcgctct gagcggcggc gttggggcag aagagctgaa agattttccg
     7081 ggctacggta aaggccttgc gccgacgacc cagttcgatg tgttgatcca cattctttct
     7141 ctgcgtcacg acgtaaactt ctctgtcgct caggcggcga tggaagcctt tggtgactgc
     7201 attgaagtga aagaagagat ccacggcttc cgttgggtgg aagagcgtga cctgagcggc
     7261 tttgttgacg gtacagaaaa cccggcgggt gaagagacgc gccgcgaagt ggcggttatc
     7321 aaagacggcg tggatgccgg cggcagctat gtgtttgtgc agcgttggga gcacaacctg
     7381 aagcagctca accggatgag cgttcacgat caggagatga tgatcgggcg caccaaagag
     7441 gccaacgaag agattgacgg cgacgaacgt ccggaaacct ctcacctcac ccgcgttgat
     7501 ctgaaagaag atggcaaagg gctgaagatt gttcgccaga gcctgccgta cggcaccgcc
     7561 agtggcactc acggtctgta cttctgcgcc tactgcgcgc gtctgcataa cattgagcag
     7621 caactgctga gcatgtttgg cgataccgat ggtaagcgtg atgcgatgtt gcgcttcacc
     7681 aaaccggtaa ccggcggcta ttacttcgcg ccgtcgctgg acaagctgat ggcgctgtaa
     7741 gcgtttctga ttctatgatt aaaggccagc cagtaacaaa actgactggc ctttttatta
     7801 cttttgcttc aacgccgcat acacctgatc aactacccaa ccataagttg caatcggtaa
     7861 ctgaccgctt tcgaacatat tgggattttt ttgtggatcg gcaactggcg aatggggctt
     7921 gttgcttaac atcacaatcg ccatatgatt gaccggatcg ataacggtta ctgtcccggt
     7981 ccagccagtg tgaccgtagg tttgcgggct tgccagcgtg ccaaacgtcg gcgtcatggt
     8041 ggcattacca ttcacgcgcc agccgaggcc aaaagtggca tcttccttag agctggtggt
     8101 gaacatcttc accgtttccg cactgaacag ctgcacatcg ccatagcccc cgccgttcag
     8161 catcgtttgc attaacaccg caatatcgcc ggtattggaa aacaaacctg cgtgcccgga
     8221 aacgccgccc atcgaataga aggctttttc atcgtgcacc tgaccccaga gagtggaggt
     8281 gcggatattc gggaaatgga tcacgccatc gcgggtattg ccgttcaatt ccgtggcggc
     8341 aatttgttgc ggtttaaagc ctttcagcag cgggttaaat accgtatgtg tcaggccgag
     8401 cgggcgataa atcgactctt caacatagcg gtcaagcggc tgaccggtaa ccgactcgac
     8461 gataaatcca agcagcatat aatcgacatc gctgtagata tgtttgctgc cgggctgata
     8521 ttccagcggc gtgcgcttga tcatctccag cgtctggcct ttatcctagg aatataacgc
     8581 gcccgcgacc gctttattcg ggtattgcgg atctgccggg aaaccgccgc tgtgatgcag
     8641 caggtcagaa atccgcaggg tgtttttgcc tttaatggtg tcatttggac tatcggcaaa
     8701 ccccggaata tatttcgcaa tccgatcgtc aggatgcagt ttgccttcgc tcatcagctt
     8761 ttgcagggcg aagttcgtgg cgtacatttt ggtgtttgag gccaggtcat acagcgtccc
     8821 ggtggtggct ttgacaggct gttccatcaa cacgctgcca tcgtactttt tcgccgctcc
     8881 ccaggctttg cgatacacaa tctgattatc tttaatgatc agcaggttta cgccgggata
     8941 accggcatca acttgctggc taatccagcg atccatctgg ttaagccgtt tgacgttaaa
     9001 ccctgctttc tctggcgagc tttctgtcag aacagggtat tttgcggcgc tgacgctaca
     9061 actaacagcc agcagagaaa gatagagcat tgtccgtttc atgatatcag tccagattga
     9121 cgttacggca gccaaagagc gtggtgaaaa taaacccgca aacccatgcc accagaatcc
     9181 cgccagcata aaccgccatg gccggaagga tgccttgtgc ggaagtcatc agcggcagcg
     9241 ctaccagacc agacggccca aaggcgctgt ttaagcccat cggcagaccc caccaggcta
     9301 tcaggccgat aaacaaaccg cccgccgcac cgcctaaaca ggcggtgaca aacggcttca
     9361 tgcggggcag ggtgacaccg taaatcagcg gttcaccaac gcccagcagg ccgggaataa
     9421 tcgccccgcg tacctgactg cgtagcgcac tgtgcggttg cgaccgccag tagagtgcca
     9481 gcgccgcgcc cacctggccc gcgcctgcca ttgaaaggat gggaaataag ctgttgaatc
     9541 cctggctgtc cattaacgcg aggtaaacag gaataaagcc ctgatgcacg ccaaatacca
     9601 cggcgatcag gaacagcccg gctaaaaccg cacaaccgaa cggattactg ttcaggtgca
     9661 taaacagcca tgacatacct tcgaataacc agccacccag cggcataatg atcaggtacg
     9721 caagcgtagc ggtgatcagc agggtgatta acgaggtcag cagcatgtcg agatcgtccg
     9781 gcataaagcg gcgcaccatg ccttcaatgc gggcgcaggc ccacgcggca atcagcacgc
     9841 cgataatatt gccgcgcgga tcgatgggca gaccaaagaa atcgtgaaaa ccggcgtagt
     9901 aaccggtggt tgcagcgggg ttataaccga gcaaaaagag tgcggcaata attgcgccat
     9961 tgacgcccgt gccgccaaat gcctgggcgg cgttatagcc caccagaatc accaggaagg
    10021 tgaacaaacc tttgctgaac accttcataa aattcagcgc gtcgggtagc gtgccctgag
    10081 catctgccgg aacgtgcatc accgtggcaa ttaacgtcgc tatccccagc agcagaccgg
    10141 cggcaataaa accggggatc agcggcgtga agatggtggc gaatttggcg agaaattgtt
    10201 gcacgccgga ggtttgtttg gcttttaact gacgtttgtt ctgggcggcg atttctgcgg
    10261 catcctgtac cggtgcctcg cccagcagct cgctcatcgc ttttgcggca cgatgcgctt
    10321 taccaggtcc aaaaacgacc tgcacctggt cgctggtcaa aatgacgctc ttcacgcctt
    10381 ccagcgtttt gatattggga tcaaccagtg aactgtcatg tacacccaga cgcaggcgcg
    10441 tcatacagtt accacaactg gcgatatttc ccggtccgcc gacacgggta agaatggtgt
    10501 tcagaagttc actgctgatc tctttggcca tgggttattc cttgtctaaa acctgacgaa
    10561 taaagccgcc gtgttgatcc aggcgttttt tagcttctgc ggcatcgaga tttttcagca
    10621 ccatcacaat ggccgtttta cagttgcgct cgcaggcaat taacgccgct tccgcttgtt
    10681 cggcattaca tccggtggcg tttttaacga tattgacctg gcgcacatgc agtttttcgt
    10741 tggtggcgac cacatcgacc ataaggttgc cgaacacttt gccggattta atcatcagcc
    10801 cggtggaaag catattgagc accagtttct gcgctgtacc tgctttcatc cgcgaagaac
    10861 cggtaacaac ttcggcacct acaatcggtg taatggcaaa ctcagcggtg gttgaaacgg
    10921 cgctccccgg attacaggaa attcccactg tgcggcagcc gagctggcgt gcgtattcca
    10981 gtccggcaat cacatacggc gtgcgaccgc tggcagcaat gccaaccacc acatcctgtg
    11041 ccgttaaatt aatatttttc agatcattaa caccgccttc ccggctatct tccgcgcctt
    11101 ccaccgcgtg ctgaatggca tattcgccgc cagcaatcaa accaaccacc agacccggtt
    11161 tcacgccgta ggtgggcgga cattcgctgg catccagaat ccccagacga ccggatgttc
    11221 ccgcaccgag gtaaatcaga cgcccgccgc cgcttacctg ggcgtggata acatcgatcg
    11281 ccgcggcgat atccggcagt acgcgctcaa cggcaagcgg tacggtttta tcttcatcgt
    11341 tgataatccg gcacatttcc agcgttgata cgcggtcaat ttcagccgag gcggcgttcg
    11401 agccttcagt aatcatcttt tcaagttgca tcggtggtga ccttactatt tacgatcaaa
    11461 taattgcaga tgtatggaat catatatcct gaattgacag acttaaagga atcattgagt
    11521 ctacactcgc agagtgtgaa gagcgtcata agaacttaag cggaatttat gctgccagcc
    11581 caggagagtg gaagatgttg tacctgacga aaataagcaa tgcggggagt gaattcaccg
    11641 aaaacgagca aaaaatcgct gactttctgc aggccaacgt gagtgagctg caatccgttt
    11701 cttcacggca gatggcgaaa cagctcggca ttagccagtc gagcatagtg aaatttgccc
    11761 aaaaactcgg cgcgcaagga tttactgaat tgcgtatggc gttaataggc gaatacagtg
    11821 ccagccggga gaaaacgaat accacggcgc tgcacctgca cagttcgatt accagtgatg
    11881 attcgctgga agtgattgcc cgcaaattga atcgcgaaaa agagctggcg ctggagcaaa
    11941 cctgtgcgtt gttcgactac gcgcggctgc aaaaaatcat tgaggtcatc agtaaagcac
    12001 cgtttatcca gataacgggc ctgggcggat cggcgctggt ggggcgcgat ctgtcgttca
    12061 aactgatgaa aattggctat cgcgtggcct gcgaagccga cacgcacgta caagccaccg
    12121 tttcccaggc gttgaaaaaa ggcgatgtac aaatcgctat ctcttacagc ggtagtaaaa
    12181 aagagattgt cttgtgcgta gaagcggcac gaaagcaggg ggcaacggtg attgccatta
    12241 cttctctggc tgactctccg ctgcggcggc tggcgcattt cacgctcgat accgtttccg
    12301 gtgaaaccga atggcgtagc tcgtcgatgt ctacccgcac ggcgcaaaat tctgttaccg
    12361 atttactgtt tgttggtctt gtgcaactca atgatgtggc gtcattgaaa atgattcagc
    12421 gcagcagtga gctaacacaa cgcctgaaat gatgcataaa gcagcgactg gattgagatt
    12481 ttcctgaatt agtgagctga tccgcagcaa tattttgttt atcctgtatt ttcagaggga
    12541 atggagtgta acgctctgta ttaacaagga gagcattaaa atgggtaaac tcacgggcaa
    12601 gacagcactg attacgggcg cattgcaggg aattggcgaa ggaattgcca gaacttttgc
    12661 acgtcatggc gcgaacctaa tcttgctgga tatctcccct gagatcgaaa agctggcgga
    12721 cgaactgtgt ggtcgtggtc atcgctgtac ggcggttgtc gccgatgtgc gtgacccggc
    12781 gtcggtagcc gcagctatca aacgcgcgaa ggaaaaagaa gggcgcattg atatcctggt
    12841 gaataacgca ggcgtttgtc gtctgggcag tttcctcgat atgagcgatg acgatcgcga
    12901 tttccatatt gatatcaata ttaaaggcgt atggaacgtc acgaaggcgg tgctgccgga
    12961 gatgattgcg cgcaaagatg gtcgcattgt gatgatgtct tcagtcactg gtgatatggt
    13021 ggccgatcct ggcgaaacgg cgtacgcctt aacgaaagcg gcgattgttg gcctgacaaa
    13081 atcgctggcg gtggagtacg cgcagtctgg tattcgcgtt aacgccattt gcccgggata
    13141 cgtgcgcaca ccaatggcgg aaagcattgc ccgccagtcg aacccggaag atccagagtc
    13201 ggtgctgact gaaatggcga aagcaatccc gatgcgtcgc ctcgccgatc cgctggaagt
    13261 cggcgaactg gcggccttcc tcgcatcgga tgaatccagc tatttaaccg gtacacagaa
    13321 tgtgattgat ggcggcagca cactgccgga gacggttagc gtcggtatct gattcacctc
    13381 tgtttcctcc ctgcatgtgt ggggaggatt tcgtcttgaa ctaagttcac caggctattt
    13441 tatttgtcat tttggccccg ggcagtgctc gaaatactca cgtactatgt gtacgctccg
    13501 gtttctccgc gctgtccgtg tccaaactgc ctgcaacaat tacgcctgtt gaaccaagtt
    13561 cttattccct ttttaacttc caaatcacca aacggtatat aaaaccgtta ctcctttcac
    13621 gtccgttata aatatgatgg ctattagaaa gtcattaaat ttataagggt gcgcaatggc
    13681 cgttaactta ctgaaaaaga actcactcgc gctggtcgct tctctgctgc tggcgggcca
    13741 tgtacaggca acggaactgc tgaacagttc ttatgacgtc tcccgcgagc tgtttgccgc
    13801 cctgaatccg ccgtttgaac agcaatgggc aaaagataac ggcggcgaca aactgacgat
    13861 aaaacaatct catgccgggt catcaaaaca ggcgctggca attttgcagg gcttaaaaac
    13921 cgacgttgtc acttataacc aggtgaccga cgtacaaatt ctgcacgaca aaggcaagct
    13981 gatcccggct gactggcagt cgcgcctgcc gaataatagc tcgccgttct actccaccat
    14041 gggcttcctg gtgcgtaagg gcaacccgaa gaatatccac gactggaacg acctggtgcg
    14101 ctccgacgtg aagctgattt tcccgaaccc gaaaacgtcg ggtaacgcgc gttataccta
    14161 tctggcggca tggggcgcag cggataaagc tgacggtggc gacaaagcca aaaccgaaca
    14221 gtttatgacc cagttcctga aaaacgttga agtgttcgat actggcggtc gtggcgcgac
    14281 caccactttt gccgagcgcg gcctgggcga tgtactgatc agcttcgagt cggaagtgaa
    14341 caacatccgt aaacagtatg aagcgcaagg ctttgaagtg gtgattccga aaaccaacat
    14401 tctggcggaa ttcccggtgg cgtgggtcga taaaaacgtg caggccaacg gtacggaaaa
    14461 agcagcaaaa gcctacctga actggctcta cagcccgcag gcgcagacca tcatcaccga
    14521 ctattactac cgcgtaaata acccggaagt catggacaaa ctgaaagata aattcccgca
    14581 gaccgagctg ttccgcgtgg aagacaaatt tggctcctgg ccggaagtga tgaaaaccca
    14641 cttcaccagc ggcggcgagt tagacaagct gttagcggcg gggcgtaact gatgtttgca
    14701 gtttcatcaa gacgcgtgct gccgggcttt accttaagcc tcggcaccag tctgctgttt
    14761 gtgtgcctga ttttgctgct gccgctctcc gcgctggtga tgcagctggc tcagatgagc
    14821 tgggcgcagt actgggaggt gatcaccaac ccacaagtgg tcgcggccta caaagtgacg
    14881 ctgctgtcgg cgtttgtggc atcgattttt aacggcgttt tcggtctgct gatggcgtgg
    14941 attttaacac gttatcgctt tccgggccgc acgctgcttg atgcgctgat ggatttaccc
    15001 tttgcgctgc caacggcagt cgccggttta acgctggcct cgctcttttc cgtaaacggt
    15061 ttttacggtg aatggctggc gaagtttgat atcaaagtca cctatacctg gcttggtatt
    15121 gcggtggcga tggcctttac cagcattccg tttgtggtgc gtaccgtgca gccggtgctg
    15181 gaagagttag gcccagaata tgaagaagcg gcggaaacgc ttggtgcaac gcgctggcag
    15241 agtttctgca aagtggtgct gccggaactt tctccagcgc tggtggcggg cgtggcgttg
    15301 tcgtttaccc gtagtcttgg tgagtttggc gcggtgatat ttatcgccgg gaacatcgct
    15361 tggaagacgg aagtgacgtc gctgatgatt tttgtgcgct tgcaggagtt tgattacccg
    15421 gcggcgagcg cgattgcttc ggtgatcctc gcggcatccc tgctgctgct gttctcaatt
    15481 aacactctgc aaagtcgctt tggtcggcgt gtggtaggtc attaatggcg gaagttaccc
    15541 aattgaagcg ttatgacgcg cgcccgatta actggggcaa atggtttctg attggcatcg
    15601 ggatgctggt atcggcgttc atcctgctgg tgccgatgat ttatatcttc gtacaggcgt
    15661 tcagcaaagg gctgatgccg gttttacaga atctggccga tccggacatg ctgcacgcca
    15721 tttggctgac ggtgatgatt gcgctgattg ccgtaccggt gaacctggtg ttcggcattc
    15781 tgctggcgtg gctggtaacg cgctttaact tccccggacg ccagttactg ctgacgctac
    15841 tggacattcc gtttgccgta tcgccggtgg ttgcaggtct ggtgtatctg ctgttctacg
    15901 gctctaacgg tccgctgggc ggctggctgg atgaacataa tttgcagatt atgttctcct
    15961 ggccgggaat ggtgctggtc accatctttg tgacctgtcc ttttgtggtg cgcgagctgg
    16021 tgccggtgat gttaagccag ggcagccagg aagacgaagc ggcgattttg cttggcgcgt
    16081 ctggctggca gatgttccgt cgcgtcacat tgccgaacat tcgctgggcg ctgctgtatg
    16141 gtgtggtgct aaccaacgcc cgcgcgattg gcgagtttgg tgcggtgtcg gtggtttccg
    16201 gctcgattcg cggcgaaaca ctttcgctgc cgttacagat tgaattgctg gagcaggact
    16261 acaacaccgt cggctccttt accgccgccg cgctgttgac gctgatggcg attatcaccc
    16321 tgtttttaaa gagtatgttg cagtggcgtc tggagaatca ggaaaaacgc gcgcagcagg
    16381 aggagcatca tgagcattga gattgccaat attaagaagt cgtttggtcg cacccaggtg
    16441 ctgaacgata tctcactgga tattccttct ggtcagatgg tcgcgctgct ggggccgtcc
    16501 ggttccggaa aaaccacgct gctgcgcatt attgccgggc tggagcatca aaccagcggg
    16561 catattcgct tccacggtac cgacgtgagc cgcctgcacg cacgcgatcg taaagtcggt
    16621 ttcgtgttcc agcattacgc gctgttccgt catatgacgg tgttcgacaa tatcgctttt
    16681 ggcctgacgg tgctgccacg tcgcgagcgc ccgaatgccg cagccatcaa agcgaaagtg
    16741 acaaaattgc tggagatggt ccagcttgcg catctggcgg atcgttatcc ggcacagctt
    16801 tccggcggcc agaaacagcg cgtggcgctg gcgcgcgcgc tggctgttga accgcaaatt
    16861 ctcctgcttg atgaaccgtt tggcgcgctg gatgcgcagg tgcgtaaaga gctgcgtcgc
    16921 tggctgcgtc aactgcatga agagctgaaa ttcaccagcg tgttcgtgac ccacgaccag
    16981 gaagaagcga ccgaagtagc tgatcgtgta gtggtgatga gccagggcaa tatcgagcag
    17041 gctgatgcgc cggaccaggt atggcgcgaa ccagcgaccc gatttgtgct cgaatttatg
    17101 ggcgaagtga accgcctgca gggaaccatt cgcggcgggc agttccatgt tggcgcacat
    17161 cgctggccgc tggggtatac gcctgcgtat caggggccgg tggatctctt cctgcgcccg
    17221 tgggaagtgg atatcagccg ccgtaccagt ctcgattcgc cgctgccggt acaggtactg
    17281 gaagccagcc cgaaaggtca ctacacccaa ttagtggtgc agccgctggg gtggtacaac
    17341 gaaccgctga cggtcgtgat gcatggcgac gatgccccgc agcgtggcga gcgtttattc
    17401 gttggtctgc aacatgcgcg gctgtataac ggcgacgagc gtatcgaaac ccgagatgag
    17461 gaacttgctc tcgcacaaag cgcctgatag gttgagtgaa tgttaaacgc ccggaggcgc
    17521 ttcccgcgat ccgggctttt taatggcaag gtttgtaacc tgtagacctg ataagacgcg
    17581 caagcgtcgc atcaggcaac accacgtatg gatagagatc gtgagtacat tagaacaaac
    17641 aataggcaat acgcctctgg taaagttgca gcgaatgggg ccgaataacg gcagtgaagt
    17701 ctggttgaaa ctggaaggca ataacccggc aggttcggtg aaagatcgtg cggcactttc
    17761 gatgatcgtc gaggcggaaa agcgcgggga aattaaaccg ggtgatgtgt taatcgaagc
    17821 caccagtggt aacaccggca ttgcgctggc aatgattgcc gcgcttaaag gctatcgcat
    17881 gaaattgctg atgcccgaca acatgagcca ggaacgccgt gcggcgatgc gtgcttatgg
    17941 tgcggaactg attcttgtca ccaaagagca gggcatggaa ggtgcgcgcg atctggcgct
    18001 ggagatggcg aatcgtggcg aaggaaagct gctcgatcag ttcaataatc ccgataaccc
    18061 ttacgcccat tacaccacta ccgggccgga aatctggcag caaaccggcg ggcgcattac
    18121 ccattttgtc tccagcatgg gaacaaccgg cactatcacc ggcgtttcac gctttatgcg
    18181 cgaacaatcc aaaccggtga ccattgtcgg cctgcagccg gaagaaggca gcagtattcc
    18241 cggcattcgc cgctggcctg cggaatatct gccggggatt ttcaacgctt ctctggtgga
    18301 tgaggtgctg gatattcatc agcgcgatgc ggaaaacacc atgcgcgaac tggcggtgcg
    18361 ggaaggaata ttctgtggcg tcagctcagg cggcgcggtt gctggagcac tgcgggtggc
    18421 aaaagctaac cctggtgcgg tggtggtagc gatcatctgc gatcgtggcg atcgctacct
    18481 ctccaccgga gtgtttgggg aagagcattt tagccagggg gcggggattt aagattattg
    18541 cgcaggcacc gttttatccg tctgcgcaat tagcgcatgt aaaaaaggct gtgcgttctc
    18601 atcgctctta ccggggatct tcacaatata cggcttcccg gtaatcgatg acgacgaagc
    18661 aaccttatca ataaactgct cggcagtgtc aatgcggtta cgggtattgc cgagcttcag
    18721 acgcagatga gaaaccgctt catcgcaggt atgttcatca ccgttgcgca caaatatcaa
    18781 atcctttttc tgtgataatc cctccagcat ggcgttgatg cgggcttctt catgggcagt
    18841 taacttcgcg taagcgggga gtgtcattag tagtgtaatg accagacaaa tgattttctt
    18901 cattgaatta ccttatcctt gttttgtaat agccgatttg actgccagaa tcaggtaaag
    18961 tttcggttgg aatacgatga ggatcatcgg caaggagaaa aaattaaatg agtagtttgt
    19021 tgttgtttaa cgataagagc agggcgctgc aggcggatat cgtcgccgtg cagtcgcagg
    19081 tggtttacgg cagcgtgggc aacagcattg ccgtgcctgc tatcaaacag aacggtctga
    19141 atgtttttgc cgtgccgacg gtattgctga gcaatacgcc gcattatgac actttctacg
    19201 gtggtgcgat tccggatgaa tggtttagcg gttatttgcg tgcgcttcag gagcgtgatg
    19261 cgctgcgcca gctgcgcgct gtaaccacgg gctatatggg aacggcatcg caaatcaaaa
    19321 tccttgccga gtggctgact gcgctacgca aagaccatcc tgacctattg atcatggtcg
    19381 atccggtgat tggcgatatt gatagcggaa tttatgtcaa acctgacctt cccgaggcgt
    19441 atcgacaata tttactgccg ctggcgcagg gaattacccc caatatcttt gagctggaaa
    19501 tcctgaccgg taaaaattgc cgcgatctcg acagtgccat tgctgccgca aaaagtctgc
    19561 tttcagacac attaaaatgg gtggtgatta ccagcgcctc cggtaatgaa gaaaatcagg
    19621 agatgcaggt ggtggtggtc agtgccgaca gcgtgaatgt catttcccat tcacgggtaa
    19681 aaaccgacct gaaagggact ggcgacctgt tttgtgctca gcttatcagt ggtttgctga
    19741 aagggaaggc gttaaacgat gcggtgcacc gtgcggggtt gcgcgtactg gaagtgatgc
    19801 gctacaccca gcagcatgag agcgatgaat tgattttgcc gccgctggcg gaagcataaa
    19861 aaatggcgcc gatgg
//
LOCUS       DAJTMC010000068        19842 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.068, whole genome shotgun sequence.
ACCESSION   DAJTMC010000068 DAJTMC010000000
VERSION     DAJTMC010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19842
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.068"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(<1..467)
                     /gene="yecA"
                     /locus_tag="OO130_002138"
     CDS             complement(<1..467)
                     /gene="yecA"
                     /locus_tag="OO130_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HCR5365550.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTNHAILDVAELDDLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERV"
     gene            complement(663..749)
                     /locus_tag="OO130_002139"
     tRNA            complement(663..749)
                     /locus_tag="OO130_002139"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(713..715),aa:Leu,seq:taa)
     gene            complement(762..835)
                     /locus_tag="OO130_002140"
     tRNA            complement(762..835)
                     /locus_tag="OO130_002140"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(801..803),aa:Cys,seq:gca)
     gene            complement(890..965)
                     /locus_tag="OO130_002141"
     tRNA            complement(890..965)
                     /locus_tag="OO130_002141"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(930..932),aa:Gly,seq:gcc)
     gene            complement(1117..1665)
                     /gene="pgsA"
                     /locus_tag="OO130_002142"
     CDS             complement(1117..1665)
                     /gene="pgsA"
                     /locus_tag="OO130_002142"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HCR5365551.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(1722..3554)
                     /gene="uvrC"
                     /locus_tag="OO130_002143"
     CDS             complement(1722..3554)
                     /gene="uvrC"
                     /locus_tag="OO130_002143"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HCR5365552.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEDEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(3551..4207)
                     /gene="uvrY"
                     /locus_tag="OO130_002144"
     CDS             complement(3551..4207)
                     /gene="uvrY"
                     /locus_tag="OO130_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HCR5365553.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            4502..4678
                     /locus_tag="OO130_002145"
     CDS             4502..4678
                     /locus_tag="OO130_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5365554.1"
                     /translation="MIKIFIGHYINVFYSTADITLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            4665..4889
                     /locus_tag="OO130_002146"
     CDS             4665..4889
                     /locus_tag="OO130_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HCR5365555.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(4956..5678)
                     /gene="sdiA"
                     /locus_tag="OO130_002147"
     CDS             complement(4956..5678)
                     /gene="sdiA"
                     /locus_tag="OO130_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HCR5365556.1"
                     /translation="MQDTDFFSWRRTMLLRFQRMEAAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPESWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFSE
                     AQPLWEAAHAHGLRRGVTQYLMLPNRALGFLSFSRCSTREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILKWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
     gene            complement(5908..6660)
                     /gene="yecC"
                     /locus_tag="OO130_002148"
     CDS             complement(5908..6660)
                     /gene="yecC"
                     /locus_tag="OO130_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HCR5365557.1"
                     /translation="MSAIEVKNLVKKFQGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     LGRIVEQGAAKALFADPQQPRTRQFLEKFLLQ"
     gene            complement(6657..7325)
                     /gene="tcyL"
                     /locus_tag="OO130_002149"
     CDS             complement(6657..7325)
                     /gene="tcyL"
                     /locus_tag="OO130_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HCR5365558.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPILSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(7340..8326)
                     /gene="dcyD"
                     /locus_tag="OO130_002150"
     CDS             complement(7340..8326)
                     /gene="dcyD"
                     /locus_tag="OO130_002150"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HCR5365559.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLNLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(8431..9231)
                     /gene="tcyJ"
                     /locus_tag="OO130_002151"
     CDS             complement(8431..9231)
                     /gene="tcyJ"
                     /locus_tag="OO130_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5365560.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(9319..9870)
                     /gene="fliZ"
                     /locus_tag="OO130_002152"
     CDS             complement(9319..9870)
                     /gene="fliZ"
                     /locus_tag="OO130_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HCR5365561.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQAHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDENGWQVEQQSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(9916..10635)
                     /gene="fliA"
                     /locus_tag="OO130_002153"
     CDS             complement(9916..10635)
                     /gene="fliA"
                     /locus_tag="OO130_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HCR5365562.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLSTKLGKL"
     gene            complement(10955..12661)
                     /locus_tag="OO130_002154"
     CDS             complement(10955..12661)
                     /locus_tag="OO130_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HCR5365563.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRILELTVQASTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGQTITIDL
                     KKIDSDTLGLNGFNVNGKGTIANKAATVSDLTAAGATGTGPYAVTTNNTALSASDALS
                     RLKTGDTVTTTGSSAAIYTYDAAKGNFTTQATVADGDVVNFANTLKPAAGTTASGVYT
                     RSTGDVKFDVDANGDVTIGGKAAYLDATGNLSTNNAGIASSAKLSDLFASGSTLATTG
                     SIQLSGTTYNFGAAATSGVTYTKTVSADTVLSTVQSAATANTAVTGATIKYNTGIQSA
                     TASFGGANTNGAGNSNDTYTDADKELTTTASYTINYNVDKDTGTVTVASNGAGATGKF
                     AATVGAQAYVNSTGKLTTETTSAGTATKDPLAALDEAISSIDKFRSSLGAIQNRLDSA
                     VTNLNNTTTNLSEAQSRIQDADYATEVSNMSKAQIIQQAGNSVLAKANQVPQQVLSLL
                     QG"
     gene            12927..14333
                     /gene="fliD"
                     /locus_tag="OO130_002155"
     CDS             12927..14333
                     /gene="fliD"
                     /locus_tag="OO130_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HCR5365564.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLTIQQGGDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAKNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKAQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSSYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKKQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            14358..14768
                     /gene="fliS"
                     /locus_tag="OO130_002156"
     CDS             14358..14768
                     /gene="fliS"
                     /locus_tag="OO130_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HCR5365565.1"
                     /translation="MYAAKGTQAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSLIQDPV"
     gene            14768..15134
                     /gene="fliT"
                     /locus_tag="OO130_002157"
                     /pseudo
     CDS             14768..15134
                     /gene="fliT"
                     /locus_tag="OO130_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015018.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
     gene            15212..16699
                     /gene="amyA"
                     /locus_tag="OO130_002158"
     CDS             15212..16699
                     /gene="amyA"
                     /locus_tag="OO130_002158"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HCR5365566.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYNLFDLGEFDQKGSIPTKYGDKVQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVITDEHGEATFFCNGGSVSVWVIEEVI"
     gene            complement(16733..17146)
                     /locus_tag="OO130_002159"
     CDS             complement(16733..17146)
                     /locus_tag="OO130_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5365567.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLALMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            17333..18538
                     /gene="yedE"
                     /locus_tag="OO130_002160"
     CDS             17333..18538
                     /gene="yedE"
                     /locus_tag="OO130_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HCR5365568.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFTALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            18535..18768
                     /gene="yedF"
                     /locus_tag="OO130_002161"
     CDS             18535..18768
                     /gene="yedF"
                     /locus_tag="OO130_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HCR5365569.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVRDIQQDGPTIRYLIQK"
     gene            18875..19546
                     /locus_tag="OO130_002162"
     CDS             18875..19546
                     /locus_tag="OO130_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HCR5365570.1"
                     /translation="MCGRFAQSQTREDYLALLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPFFIYRADGQPIFMAAIGSTPFEHGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAAQEWMRQEIGGKEASEIATNGCVPANQFTWHPVSRAVGNVKNQGAEL
                     IQPVC"
     gene            complement(19586..>19842)
                     /locus_tag="OO130_002163"
     CDS             complement(19586..>19842)
                     /locus_tag="OO130_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5365571.1"
                     /translation="GGQVFAVPPACTSQRCACCGHTAKENRLSQSKFRCQVCGYTANA
                     DVNGARNILAAGHAVLACGEMVQSGRPLKQEPTEMIQATA"
BASE COUNT         4895 a         5177 c         4831 g         4939 t
ORIGIN      
        1 acccgctcga agttttcctc agtaccgtgc agcgcaatcg cctccagcgc tggttttaac
       61 gaatcgggca acgttgacca gtcagaaagt gccacgcccc gcatatagcc aaagcaccac
      121 tcctcaacaa tcgtcagctc gctgccatca acttctcgca agccgaataa cggctcaaac
      181 tgctccggga attcgttcag acgctctgcg gtatcggcca tatgttgaaa agccaggttc
      241 ataaagcgcg tcatctcttt ctctgacgcc cagcgcggca catagtcagc cccaccccac
      301 acggcaacca gccactgttc cggttcaatc tcttgcggag aactcaacac cgccgtcaat
      361 aaatcgtcca gctccgccac atcaaggatg gcgtggttag tgttgtattt ggtcagaata
      421 tcgtccagcc attccaactc actttcgttt aacggtcccg ttttcatacg cttttccttg
      481 tggatctcaa ctcgccagca cctatcttac atgccggtcc gtatcagaga tactttttga
      541 gtggctttgc cggtgattaa aaattaagga gggtgtaacg acaagttgca ggcacaaaaa
      601 aaccacccga aggtggtttc acgacactgc ttattgcttt gattttattc ttatctttcc
      661 catggtaccc ggagcgggac ttgaacccgc acagcgcgaa cgccgaggga ttttaaatcc
      721 cttgtgtcta ccgattccac catccgggct cgggaagaaa gtggaggcgc gttccggagt
      781 cgaaccggac tagacggatt tgcaatccgc tacataaccg ctttgttaac gcgccaaatt
      841 cttcaggcct ttcagccaga catccgcttg acgccgatgt cttttaaact ggagcgggaa
      901 acgagactcg aactcgcgac cccgaccttg gcaaggtcgt gctctaccaa ctgagctatt
      961 cccgcattca ttaagcaatc agttaatcac ttgattttat tatcgtctgg caatcagtgc
     1021 cgccgttcga tgcgttgcat tctacttacc tggcgcgatg agtcaacgat atttttcacc
     1081 acttttgatc gtttgctgaa aattacgccg aaacgatcac tgatcaagca aatctgcacg
     1141 cgcagcgctc aaatattgca acattgacca cagagtcagc accgcagcca caaagaaaag
     1201 tgcaataccg gcgtactcaa cccaaatgtt cggacgccac agcagccagg ccaacgccac
     1261 catctgggca gtggttttca ctttcccaat ccaggagaca gccacgctac tgcgtttacc
     1321 caactccgcc atccattcgc gtagcgcaga aataataatt tcacgggcga tcatcgttgc
     1381 cgccggtagg gtcacccacc agctgtgata atgctcggtt accagcacca tggcgatagc
     1441 cacgagaact ttatctgcta cagggtcaag gaaagcacca aaccgggtac tctggttcca
     1501 gcggcgtgcc agaaaaccat cgaaccagtc agtcaccgcc gcgacgcaga aaatgagcgc
     1561 ggcggcaaac ggcgaccagg tgacaggcag ataaaagacc aatacaaaga atgggataag
     1621 gatgacacgg aacagtgtaa gcaacgtagg gatattaaat tgcataatga cgggtaacta
     1681 tctgttgtca gtaagattac ccctatgttg ctacagagac atcaatgttt caacgaccag
     1741 aagatctttt ctgccagacc ttgcgaaata cccggcactt ttgcaatttc ctcgacgctg
     1801 gcgttacgta aaccttgcaa accgcccata tatttcaaca acatttgccg acgttttggc
     1861 ccgacgcctt caatggtttc cagggaactg gtatttttga ccttcgcccg ttttttacgg
     1921 tgcccgccaa tcgcgtgatc atgtgattca tcgcgaatat gctggataac atgcagcgcg
     1981 ggtgaatctg gcggcaaact aaatccctca tcttccggct caaagaacag cgtttccagt
     2041 ccagccttac gatctgctcc tttggcaacg ccaagtagca gcggatgatt tttatcccat
     2101 gagacatcca gttcggcgaa gacatttttc gcctgcgcaa gctggccttt gccgccgtcg
     2161 ataaggatca catccgggat cttactgtcg tcaatggctt taccataacg ccgacgcagc
     2221 acctgattca tcgccgcata atcatcgccc ggcgtgatgc ctgtaatgtt atagcgccga
     2281 tactccgcac gcagcgggcc gttagcatca aacaccacac aggaagcgac ggtttgttcg
     2341 cccatggtat ggctgatgtc aaagcactcc atccgcttca cttccggcaa tttcaacacg
     2401 ctggcaagcg cggtcagtcg ctggtgaacg gtagattgct gcgaaagttt gctggttaag
     2461 gccgtcgccg cattggtgcg cgcgagtttc agataacgcg ccctatcgcc gcgaggtttg
     2521 gtttgaacat taatcttgcg tcccgccagt tctgaaaggg aatcggcgag cagcgtttta
     2581 tcgctaagat taaaatcgag caggatctca cccggtaagg tgcgcatctg gctgccttgt
     2641 aaatagaact gacctacgaa ggtttctacc acctcgctca gttccgtacc gccaggcact
     2701 ttcgggaaat agctgcggct gccgagcact ttgccctgac gaatgaacaa tacgtggaca
     2761 caagccatgc ccgcatcgaa cgccacacca ataacgtcaa ggtcgtcgcc ggtattggaa
     2821 acaaactgtt tttcggtgac gcgtcgcacc gcctgaattt ggtcgcgaat acgtgccgct
     2881 tcttcaaact ccagattctg actggcagtt tccatacggc taatgagttg cgtaagcacc
     2941 tgatcatctt tgccagacaa aaacaggcgc acatactcga cctgctgagc gtattcttct
     3001 tcactcacca gtccttcaac gcacggtccc agacagcgcc ctatctgata ttgcagacac
     3061 ggacgcgagc gattgcgata aacactattt tcgcactggc gaatggggaa aatcttttgc
     3121 agtagcgcca gagtttcacg tacggcatag ccattcggga acgggccgaa atattcacct
     3181 ttggcatgct tcgcaccacg atgcatcgcc agacgcgggt gggtatcgcc actcaggaag
     3241 ataaaaggat aagatttatc atcgcgtagc aaaacgttgt aacgcggctg atagagtttg
     3301 atgtagttgt gttccagcaa cagcgcttcg gtttctgtat gagtaaccgt tacatcaatt
     3361 tgctgaatct gggcgaccag cgcttcggtt ttgcgcgaag cgaggttgct acggaaatag
     3421 ctggaaagcc gttttttcag gtctttcgct ttgccgacat agataaccgt accaccagca
     3481 tcgtacatgc gataaacgcc tggctggctg gttacggttt ttaaaaacgc ttttgcgtca
     3541 aactgatcac tcactgactt gataatgtct ccgcattaca cagaccatgg cgaattgcca
     3601 ggtgagtcag ctcaacatcg ccatgaatgt ttagtttact gaacatacga tagcggtagc
     3661 tgttcaccgt tttcggactg agattgagct gttctgagat ctcattgacc ttctggccct
     3721 tggtgatcat cagcataatc tgcaattcac gttcagacaa actggcaaat gggctttctg
     3781 ttttttctgg ttcgatctgg cttaacgcca tttgttgagc gatgtcagaa gcaatgtaac
     3841 gctgccctga atagacagaa cgaatcgcac tcacgacttc ctgcggagcc gcgcctttgc
     3901 tgaggtagcc cgcagcaccg gcctgcatga ctttcgctgg taaagggttt tctgtatgaa
     3961 cggtaagcat gatgattttg acatcagctg tggaacgcgc gattttacgc gtcgcctcaa
     4021 gaccgccaat gcccggcata ctcatgtcca ttagcaccac gtcaacggca tttgtccggc
     4081 accacttaac ggcgtcttca ccgcacgatg cctcaccgac gacttttata ccctttatat
     4141 cttccagaat gcgtcgtatc cctgcgcgca ccagttcgtg gtcatcaaca agtagaacgt
     4201 tgatcaaaga aatatctcca gaaataggga taacgctact gatagttagt cattcgtata
     4261 ttagcggttt ttattgcaac tttgaaacgt taaaaatggt gggtattcga tttttctctc
     4321 gtttttgtca attcgattgt ccacagttat ggtcacgccc gtcaccaacg gcttaccgga
     4381 tcgtttcaca gtaagcccta cgatgaaatg tgacaaaaat tgactttatt cagcaaaaat
     4441 gaaaatcagc ccgctgaata tgtaacatta attaacccga acaatacggg caaaaacatt
     4501 catgataaaa atatttattg gtcattatat taacgtattt tatagcactg ccgatatcac
     4561 gctcaaaaaa caaccactgc tatttttagc aaagcttatg gtatactccg ccgccttaac
     4621 atttttcacc gcaaattttc attgcaacat gacgaggaaa ataaatgagt acgcctgatt
     4681 tttctactgc cgagaataat caagaactgg caaatgaagt ctcctgcctg aaagcgatgc
     4741 tgacgctgat gctgcaggcg atgggacaag ctgacgcggg ccgcgtgatg ttaaagatgg
     4801 aaaaacagct tgcgctgatc gaagacgaaa cccaggctgc agtattttcc aaaacggtta
     4861 agcaaattaa acaagcctac cgtcagtaat ataaaaccgg ctgagagcgt gcctttcagc
     4921 cggtttttgc atctggcacg cagaaagaaa agcgatcaaa ttaagccagt agcggccgcg
     4981 taacaggcca cctgggtctt atttggtgca ttgatttttt tctgcatgtt tttctgatgg
     5041 aaattgaccg tattctcaga gattgacaaa atcatcgcta tctctgctga tgtcttccct
     5101 tccgccgtcc atttcagaat ttctttttcg cgcttgctga aattcatctc tggcgtcatc
     5161 actatttcat catttaaacg catcagagcc atcagacttt cgcgcaccag taactgcatt
     5221 tttaattgca gttcatcact aagaatgggt atttcgcgcg tgctgcaacg ggaaaaggac
     5281 aaaaagccca gcgcccgatt tggcagcatt aaatactgag tgacaccgcg gcgtaaacca
     5341 tgtgcgtgcg cggcttccca taatggctgt gcttcgctga ataagtcatc attccacatt
     5401 aaatggccct gactaaagtt ttcagggttc agcacaggat caattgcgag aaaatttttt
     5461 gcctgataat aactaaccca cgactcaggg taattggtgt agaaagccac tttaggtcgc
     5521 gtgaatggta ccgggtggcg aacacataac gaatagtaat cgtactccag ctgctgagcc
     5581 tgaagctcta tttcatggta gacctcttct gcggcctcca tcctctgaaa acgcaacagc
     5641 atcgtgcgac gccaactgaa aaaatccgta tcctgcatag taaaccgcaa cgcccctgag
     5701 agtgagtatc atttataatg ataattcaaa ctatcacata aataagattt atatataatt
     5761 tatattattc aggcaatgaa ttacttttgc aagccatcgc attctcttat gttattaatg
     5821 agttatgctg atttgttaag cagttttatc acgcttgaaa tggcgtcccg ccccgacagg
     5881 tgaatcgtcg gggctgattt tttcttatta ttgcagcaga aacttctcga ggaactgtcg
     5941 ggtgcgaggc tgctggggat cggcaaataa cgcttttgct gccccctgct cgactatccg
     6001 ccccaggtcc ataaaaatcg cccggtccgc aacatcccgg gcaaagctca tttcgtgcgt
     6061 cacaatcacc atcgtgcgct tttcctgcgc cagctggcgg attgtgttca ggacttcgcc
     6121 caccagctct ggatccagcg ctgacgttgg ctcgtcaaac aaaatcacct caggacgcat
     6181 tgccagcgca cgcgcaatcg ctacacgctg ctgttgaccg ccagacaaac gacgtggata
     6241 gctggtttct ttaccggcca gcccaacttt cgccagcagc tcgcgagcgc gcgccgtggc
     6301 ctcttctttc ggttcgcctt tgacgatcac cggcccttca ataatgtttt ccagcaccgt
     6361 acgatgcgga aacaaattaa agttctggaa gacaaacccg acgtgctgac gcaactggcg
     6421 aatcagcgat ttttgctgac ttaatgaacg tgcagtatca atagtgatat cgccaacggt
     6481 gatcgttccc gcttccggtt gttccagcag atttatgctg cgtagcaacg tggttttgcc
     6541 ggaaccactc ggaccgataa ttgccaccac ttcgccaggc tttacctcga ggtcgatacc
     6601 gtgcagcacc gtctgacctt ggaatttttt caccaggttc ttaacttcaa tggcactcat
     6661 ttgggttctc tctcctggcg attaagttga ttctcaaaat ggttctgcaa cgtcgataac
     6721 acagtcgcca tgatccagta gatgagcgaa gccgccaggt acatggtgaa cacctccagc
     6781 gtgcgcgagg taatcaactg cgcctgacgg aacagctccg gcacctggat tgtcgcggcc
     6841 agcgacgtat cttttaccag actgataaag ctgttcgaca gcggcggcaa cgccacccgc
     6901 gccgcctgtg gcaaaatcgc gcgacgcatg gtctgccacg gcgtcatacc aatactggca
     6961 gcggcttccc actgaccttt atcaatagaa gaaatggcgg cgcgcagcgt ttcggcggca
     7021 taggcggcag tgttcagcga cagaccaatc attgctgaca gaatgggatc taattcgata
     7081 ccaaactgcg gcaggccgta atagatcata aacagttgtg cgatgagcgg cgtaccacga
     7141 aaaatagaga tataaaaacg cgccagccag cgcacgggcc agattggcga caggcgcatc
     7201 agcgcgagaa taaacccgag tagtaagcca aaaaacatgc cgccgatact gagttgcagg
     7261 gtatacccag cccctttcaa caggaacggc aaggaatcaa taaccagttg tatactttct
     7321 tgcatgagcg ttcccggagc taaacgtggg gatgataggc gaacagcgca ggcgcgccgc
     7381 cggtatgaat aaacagaatc ggcccttcat ctttgaagcg tttctgactg ataccgtcaa
     7441 tcagccccgc catcgctttt ccggtataca caggatcaag cagaatgcct tccagacgcg
     7501 ccagcaattt cactgcttcc atgccttcgt cgttcggcac gccgtagcca ggtgcaaaat
     7561 agtcatccca gagtaaaatt tccgctgatg cggtcagctc cagttctttc gcaatcgcct
     7621 gttgtaggtt aaccactttc ggcaattgat cggcaacgga acgcgacacg gtcacgccaa
     7681 tcagttcgct ttcaggcatc aggtgttcca gcccaacagc caatccggcg tgagttccgg
     7741 cactgcccga tgcgaccacc accgacgaaa tattaaccgc cccttcacac tgttgcgcga
     7801 tttccagcgc actctccaca taacccagcg cgcccagagc attagaaccg ccaaccggaa
     7861 tgacatacgg gcgaaagcct tgtgcttcga ctcgcgtcgc cagctcttcc agttgggcat
     7921 tgggatcggt cagtgcgtcg cacatttcaa tctgggtatt gaacagattc agcaacaaac
     7981 gattgccgtt ggttaaatag ttttctgcgg ttgtgccaat aggattttcc agcagcgcca
     8041 cgcagtgcag gccgagtttc gccgcaactg cggcagtctg ccgcacatgg ttagactgga
     8101 tcgcgccggc agtaatcagc gtatcggcac cttcgcgcag ggcatctgcc gcgagaaatt
     8161 ccagcttacg taatttattg ccgcccattg ccatgggtgt gacgtcatcc cgtttgatga
     8221 aaatttcccg tccaagataa tcagaaaagc gcggcagata ttcgagcggc gttggcgcgc
     8281 cgataaactc cagccgtgga aaacgggtta aattatgcag tggcataata gcctccgatg
     8341 tgtgttgttg tgattttctt attatgcacg ctgaaaacgc gtaaataaaa aaggcgctag
     8401 tgaaagcgcc cttttttgtc attatgctga ttatttggtc acatcagcac caaaccattt
     8461 ttcggaaagg gcttgcagag tgccatcttt ttgcatttcc gcaattgcat cgttcactgc
     8521 tttcagcagg tcttcatttc ctttacgcag cgccacgcca gactcctgac gggagaatgc
     8581 ttcaccggtt actgccagcg tatcgttggt tttcttcacc agatccagcg ccgccagacg
     8641 gtcaacgagg atcgcatcga tacggcctac gcgcagatcc tgatatttgg tcgggtcatc
     8701 atcataggta cgcacatcga cgccctgaac attctgccgc agccactctt catagttggt
     8761 gcccagaccg acacccactt ttttgccttt cagatcagca gccgttttaa tggtgccttc
     8821 gttacctttt ttcaccagcg cctgaatacc agaaatggtg tacggggtag agaaatcgta
     8881 ttttttcttg cgctcatcag aaatggtgac ctgattaatc accacatcaa tacgtttaga
     8941 gtccagcgac gctagcatac cgtcccattt ggtcggtttt agtgacgcct caacgccaag
     9001 atgttttgcc agctgttggg caaattccac ttcaaaaccg gttaatttgc cgtcatcccc
     9061 ctgaaaactg aacggcggat aagttccttc cagccctacc agtagcgtac cgcgctcttt
     9121 gactttatta agcagacctt catctgcaaa acttttaacg ctcatgcccg caaccagcgc
     9181 cacggccatc acacccatca atgcctgacg tcccagatgt gctaatttca tattcacccc
     9241 gaatgttgtt atgtctgttt gcagtgtaga gccattgttt gtaaacacaa aaacaactcc
     9301 gctacatctt attcttattt aatatatatc agaagcaggc aggctggagg atttctgcac
     9361 aagtccggta cacgtttgac gctgatagtg ttgatacttc cgtaatgcga tgcggtaatt
     9421 gttggtgctg gtggtgggca gccacggggc gaggatttca tcaaggaaac cgtcctgcag
     9481 ctgatcgagg gaaatatttt gctcatgtag atgattcccc aaacggcgca aacgaacgac
     9541 atattcacgc accgtacctg ggctcatttc agtttgctca aaaagaaatt gcttaaagcc
     9601 gataatatcg aaaaaatcac tctgcgtttt gcaatgtaaa tcaccgcaaa atcggcacaa
     9661 tgccgcccat gactgttgtt ccacttgcca gccattttca tcaagcagcg tgtccaggcg
     9721 ggaaatctcg attttattca ctattttgcc gtcgcgaact aaggtaatgc gatcgagtaa
     9781 tttacggcaa tgcgcgcaat gggcctggct gtgtttaaag tctttaagat agcggcttaa
     9841 tggccgtctt ttcaggtgct gcaccatcat taagaactcc tggtagtcaa agttaaagtg
     9901 cggcatttac tgacgttata acttacccag tttagtgctt aaccgtttaa tggcctggct
     9961 gtgtaactga ctgacccgcg attccccgac ctccagcacc gcgccaatct ctttgagatt
    10021 cagctcttcc tggtaataga gggttaatac cagtttttcg cgctccggca acgtttcgat
    10081 agcttccatc acccgctggc gcagattact gtccagcagt tgttgtagcg ggttttctcg
    10141 ctgatgatca tcagtaacca gttcgatgct atcgccgtgc tcttcgcgcc actcatcgta
    10201 ggagaaaagc tggctgttat tggtgtcgag caacatttgg cgataatcgg caatatcgat
    10261 ccctaaacgt tccgctacct cagtttccgt ggcgttgcgg ccaagttcct gctccagttg
    10321 ccctattgcc tgtgccactt cacgcgcgtt gcgtcgcacg ctgcgcggca cccagtcacg
    10381 gctgcgaagt tcatccagca tagcgccacg gatacgctgc actgcgtaag ttgtaaatgc
    10441 cgttccttgt agggcgtcat agcgttcgac ggcattaagt aacccaatgc cgcccgcctg
    10501 tagcagatcg tcaagttcca cgctcgcggg cagtcgaacc tgcaggcgca atgcttcgtg
    10561 acgcaccagc gggacataac gctgccacag cgagtgttta tccattacac cttcagcggt
    10621 atagagtgaa ttcacgataa acagccctgc gttatatgag ttatcggcat gattatccgt
    10681 ttctgcaggg tttttaatcg gacgattagt gggtgaaatg aggggttatt tgggggttac
    10741 cggtaaattc catgcagaaa aaaccccgcc ggtggcgggg aagcacgttg ctgacaaatt
    10801 gcgctttatg ttgccggatg cggcgtaaac gccttatccg gcctacaaaa atgtgcaaat
    10861 tcaataaatt gcaattcacc ttgtaggcct gataagtgca gcgcatcagg caatttggcg
    10921 ttgccgtcag tctcagttaa tcaggttacg acgattaacc ctgcagcaga gacagaacct
    10981 gctgcggtac ctggttggct tttgccagca cggagttacc ggcctgctgg atgatctgcg
    11041 ctttcgacat gttggacact tcggtcgcat agtcggcgtc ctgaatacgg gactgcgctt
    11101 cagacaggtt ggtagtggtg ttgttcaggt tggtgactgc agaatccaga cggttctgaa
    11161 tagcacccag ggatgaacgg aatttgtcga tggagctgat agcttcatcc agggcagcca
    11221 gaggatcttt ggttgcagtg cctgcactgg tggtttcagt ggtcagtttg cctgtagagt
    11281 taacataagc ctgtgcccca acagtagctg caaatttacc agttgcacct gcgccatttg
    11341 aagctacagt tactgtaccg gtatccttat cgacgttgta gttgatagtg taagatgcgg
    11401 ttgtggtgag ctctttgtct gcatcagtat aggtgtcatt cgaattacca gcaccattag
    11461 tattcgcacc accgaaggac gccgttgcag actgaatacc tgtattatac ttaattgtcg
    11521 caccagtaac tgctgtgtta gccgttgcag cactctgcac tgtgctcagt acagtatcag
    11581 cgcttacagt tttggtgtag gttacgccag aagttgccgc tgcaccaaag ttataagttg
    11641 tgccagacaa ctggatagaa ccagttgtcg ctaaggtact accgctagca aacagatcgg
    11701 acaatttcgc tgaagatgca atgccggcgt tgtttgtaga taggttacca gtggcgtcca
    11761 ggtacgcggc tttaccaccg atggtcacat cgccattagc atctacatca aacttcacat
    11821 caccagtact acgagtataa acacctgatg cagtagtgcc agccgctggt ttcagagtat
    11881 tcgcaaagtt aacaacatcg ccatctgcaa ctgttgcttg agtggtgaag ttccctttag
    11941 ccgcatcata agtatagatc gcagcactcg agccagtagt agtaactgta tctccggttt
    12001 tcaggcgaga cagtgcatcg ctagcgctga gtgctgtatt gtttgtggtc acagcataag
    12061 gacctgttcc cgttgcacca gcagcggtca gatcgctgac tgtagcagct ttgttcgcaa
    12121 tagtgccttt gccattcacg ttaaaaccat tcagccccag cgtatctgag tcaattttct
    12181 tcagatcaat cgtgatagtc tggccgtcat tcgcaccaac ctgaattttc attgaaccgt
    12241 ctttcgccag tacgttcacg ccgttgaact gggtctggcc agatacgcgg tcaatttcgt
    12301 ccagacggga tttgatttcg tcctgaatgg agtccagatc cgaatcggag ttagtcccgg
    12361 tagaagcctg aaccgtcagt tcaagaatac gctgtaagtt gttgttgatt tcggacagcg
    12421 cgccttcagt ggtctgcgca acggagatac cgtcgttggc gttacgggcc gcctgagtca
    12481 ggcctttaat attagaagta aaacggttag caatcgcctg acctgcggcg tcatccttcg
    12541 cgctgttaat acgcaagcca gaagacagac gctcgataga actcgacagc gcagactggt
    12601 tcttgttgat attattttga gtgatcagcg agaggctgtt ggtattaatg acttgtgcca
    12661 tgattcgtta tcctatattg caagtcgttg attacgtatt gggtttccac ccgtcggctc
    12721 aatcgccgtc aaccctgtta tcgtctgtcg taaaacaacc tttagaattt ttttcacaaa
    12781 cagccatttt ttgttagtcg ccgaaatact cttttctctg ccccttattc ccgctattaa
    12841 aaaaaacaat taaacgtaaa ctttgcgcaa ttcaggccaa taaccccggt attcgtttta
    12901 cgtgtcgaaa gataaaagga aatcgcatgg caagtatttc atcgctggga gtcgggtcag
    12961 gtctggattt aagttcaatc cttgatagcc tcaccgccgc gcaaaaagcg acgctaaccc
    13021 ctatttcaaa tcagcaatcg tcgtttaccg ctaaacttag cgcctacggt acgctgaaaa
    13081 gcgcgctgac gactttccag accgccaata ctgcattgtc taaagccgat cttttttccg
    13141 ccaccagcac taccagcagc accaccgcgt tcagtgccac cactgcgggt aacgccatcg
    13201 ccgggaaata caccatcagc gtcacccatc tggcgcaggc gcaaaccctg accacgcgca
    13261 ccaccagaga tgatacgaaa acggcgatcg ccaccagcga cagcaaactc accattcaac
    13321 aaggcggcga caaagatccg attaccattg atatcagcgc ggctaactca tcgttaagcg
    13381 ggatccgtga tgctatcaac aacgcaaaag cgggcgtaag tgcgagcatc attaacgtgg
    13441 gtaacggtga atatcgtctg tcagtcacat caaatgacac cggccttgat aatgcgatga
    13501 cactctcggt cagcggtgat gatgcgctac aaagttttat gggctatgac gccagtgcca
    13561 gcagcaacgg tatggaggtc tcggtcgccg ccaagaatgc gcagctgacg gtcaacaacg
    13621 tcgccattga gaacagcagc aacaccatca gcgacgcgct ggaaaacatc accctgaacc
    13681 tgaacgatgt caccacgggc aaccagacgc taaccatcac tcaggacacc tccaaagcgc
    13741 aaacggcgat taaagactgg gtgaatgcct ataactcgtt aatagatacc ttcagtagcc
    13801 tgaccaaata caccgccgta gatgcgggag ctgatagcca gagttctagc aatggcgcac
    13861 tgctcggcga ctccacgctg cggacgattc agacgcagtt gaagtcgatg ctgagtaata
    13921 ccgtcagttc ttccagctat aaaacgctgg cgcagattgg tatcacgacc gatcccagcg
    13981 atggcaaact ggaactggat gccgacaaac tcaccgctgc actgaaaaaa gatgccagcg
    14041 gcgtaggtgc attgattgtt ggcgatggta aaaaaaccgg catcacgacc accatcggca
    14101 gcaacctgac cagttggctt tcgacaacgg gcattattaa agccgctacc gatggcgtta
    14161 gtaagaccct gaataagtta actaaagact acaacgccgc cagcgatcgc attgatgcgc
    14221 aggtcgcgcg ctacaaaaaa caatttaccc aactggacgt tttaatgacc tcgttaaaca
    14281 gcaccagcag ctacttaacg cagcagttcg aaaacaacag taattccaag taatcattat
    14341 tcatcgggag acaggtcatg tacgcggcaa aaggcaccca ggcctatgca caaattggcg
    14401 tcgaaagcgc cgtaatgagc gccagccagc agcagctggt caccatgcta tttgatggag
    14461 tgctgagcgc actggttaga gcgcgcctgt ttatgcagga caacaatcag caaggcaaag
    14521 gcgtctcttt gtcaaaagcg atcaacatca ttgagaacgg actgcgggtg agtcttgatg
    14581 aagagagcaa agacgaacta acccaaaact tgattgctct ttatagctat atggtcaggc
    14641 gcttgctgca agccaattta cgcaacgatg tctccgcagt cgaagaagtg gaagcattaa
    14701 tgcgcaatat tgccgatgcc tggaaagagt cgttactctc cccttctttg attcaggacc
    14761 cagtctgatg aaccatgcac cgcatttgta tttcgcctgg caacaactcg tcgaaaaaag
    14821 ccagctcatg ttacgcctgg caacggaaga acaatgggac gaactcatcg ccagcgaaat
    14881 ggcgtatgtg aatgcggtgc aggagtatag cacacctgac tgaagaggtt gccccgtcca
    14941 ccacgatgca ggagcagctc cgcccgatgc tgcgcctgat tctcgacaac gaaagcaagg
    15001 taaagcagtt attacagatt cgtatggatg aactggcgaa actggtcggt cagtcatcgg
    15061 tgcaaaaatc ggtgttaagt gcctatggcg atcagggcgg ctttgtgctg gctccgcaag
    15121 ataacttctc gtgaatctta acgagctaat ggttcgcgaa taatccgatt acggctacgc
    15181 ttctaatgtt ccccttgaat ggagtcgaag aatgcgtaat cccacgctgt tacaatgttt
    15241 tcactggtat tacccggaag gcggtaagct ctggcctgaa ctggccgagc gcgccgacgg
    15301 ttttaatgat attggtatca atatggtctg gttgccgccc gcctataaag gcgcatcggg
    15361 cgggtattcg gtcggctacg actcctataa tttatttgat ttaggcgagt ttgatcagaa
    15421 aggcagcatc cctactaaat atggcgataa agtacaattg ctggccgcca ttgacgccct
    15481 gaaacgtaat gacattgcgg tgctgttgga cgtggtagtc aaccacaaaa tgggcgcgga
    15541 tgaaaaagaa gctattcgcg tgcagcgtgt aaatgctgac gaccgtacgc aaattgatga
    15601 agaaatcatt gagtgcgaag gctggacgcg ttacaccttc cccgcccgtg ccgggcaata
    15661 ctcacagttt atctgggatt tcaaatgttt tagcggtatc gaccatatcg aaaaccctga
    15721 tgaagatggc atatttaaaa ttgttaacga ctacaccggc gaaggctgga acgatcaggt
    15781 tgatgatgaa ttaggtaatt tcgattatct gatgggcgag aatatcgatt ttcgcaatca
    15841 tgccgtgacg gaagagatta aatactgggc gcgctgggtg atggaacaaa cgcaatgcga
    15901 cggttttcgt cttgatgcgg tcaaacatat tccggcgtgg ttttataaag agtggatcga
    15961 acacgtacag gaagttgcgc caaagccgct gtttattgtg gcggagtact ggtcgcatga
    16021 agtcgataaa ctgcaaacgt atattgatca ggtggaaggc aaaaccatgc tgttcgatgc
    16081 gccgctgcag atgaaattcc atgaagcatc gcgcatgggg cgcgactacg acatgacgca
    16141 gattttcacg ggtacattag tggaagccga tcctttccac gccgtgacgc tcgttgccaa
    16201 tcacgacacc caaccgttgc aagccctcga agcgccggtc gaaccgtggt ttaaaccgct
    16261 ggcgtatgcc ttaattttgt tgcgggaaaa tggcgttcct tcggtattct atccggacct
    16321 ctacggtgcg cattacgaag atgtcggtgg tgacgggcaa acctatccga tagatatgcc
    16381 aataatcgaa cagcttgatg agttaattct cgcccgtcag cgtttcgccc acggtgtaca
    16441 gacgttattt ttcgaccatc cgaactgcat tgcctttagc cgcagtggca ccgacgaata
    16501 tcccggctgc gtggtggtca tgtcgaacgg ggatgatggc gaaaaaacca ttcatctggg
    16561 agagaattac ggcaataaaa cctggcgtga ttttctgggg aaccggcaag agagcgtaat
    16621 aaccgacgaa cacggcgaag caaccttctt ttgcaacggc ggcagcgtca gcgtgtgggt
    16681 tatcgaagag gtgatttaaa ttcatccccg gcggcaagcc ggggagattt cattacggca
    16741 gtggcgtcgg taacggcgct ttatccagcg cggcggcaca ctctgccgtc gggcgatcca
    16801 cacgctccat cgtcatacca tcgtactcaa tggtgttacc gtcacgctca acctcgtaca
    16861 gctcacgttt tacggtaacg ttagtgagat catcagacat caatgccaac ttacccggca
    16921 cagcaatcac ccgctgccac tgacggcaat ccagagtatc accctcttta gtgacaatca
    16981 ggctgcctat cgcttccgga ctgaccagcg cacgttgcgg ccctttggtc tgccagtagc
    17041 ccgccagcca gtccggcgcg ggggttttca cgacattgtt atagttttcg acttcggcac
    17101 agcccgccag caacatgagc gcgcctgcaa ttgcgagttt tttcatcatt cgtcctgcat
    17161 gagaagaaaa gaggattgtg gcattaaagc cctgatgtcg ccagcctttc caggttcaga
    17221 gagaaatttg atctaactat tatttttgtg aattattacc ccgaaaataa atttgcatga
    17281 tgtatgattc gcgactcttg tttcaaacct tctgagttca gaggctacat tcatgtcatg
    17341 gcagcaattc aaacacgcct ggttgattaa attctgggca cccatccctg cggtcatcgc
    17401 ggcgggtatt ctctccactt actattttgg cattactggc accttttggg ctgtcacggg
    17461 tgaatttacc cgttggggcg gccagctcct gcagctgttt ggcgtccatg ctgaagagtg
    17521 gggttatttt aaaattatcc atctggaagg atcgccatta acccgcatcg acgggatgat
    17581 gatcctcggc atgtttggcg gctgctttgc cgcagcgctg tgggccaaca atgtcaaact
    17641 gcgcatgcca cgcagccgta tccgcattat gcaggccatt attggcggta ttatcgctgg
    17701 ttttggcgcg cgtctggcaa tgggctgtaa cctggcggcg ttctttaccg gtattcctca
    17761 gttctcgctg catgcctggt tctttgccat cgccactgcc attggttcat ggtttggcgc
    17821 acgctttacc ctgctgccca tcttccgtat tcccgtgaaa atgcagaaag tttctgccgc
    17881 ctcaccgctg acgcaaaaac cggatcaggc gcggcgtcgt tttcgtctcg ggatgctggt
    17941 ctttttcggt atgctgggct gggcgctgct cacggcgatg aaccaaccaa aactggggct
    18001 ggcaatgctg tttggcgtcg gctttggttt actgattgaa cgtgcgcaaa tctgctttac
    18061 ttcggcgttc cgcgatatgt ggatcaccgg acgtacccat atggcgaaag caatcattat
    18121 cggtatggcg gtgagtgcca tcgggatctt cagttacgta cagttaggcg ttgaacccaa
    18181 aatcatgtgg gcgggaccaa acgcggtaat tggtggttta ctgtttggtt ttggcatcgt
    18241 gctggcaggc ggctgcgaaa ccggctggat gtaccgcgcg gtagaaggcc aggtgcacta
    18301 ctggtgggtc ggtctgggca atgtgatcgg ctcgacgatt ctggcgtatt actgggatga
    18361 tttcgctccg gcgctggcca ccgactggga caaaatcaac ctgctgaaaa cctttggccc
    18421 gatgggtggc ctgctggtga catatttgct gttgtttact gcgctgatgt tgattatcgg
    18481 ctgggaaaaa cgcttcttcc gccgtgcggc accgcagact gctaaggaga tcgcatgaaa
    18541 aatatcgttc ctgattaccg ccttgatatg gtgggtgaac cctgccctta tccggcagtc
    18601 gcaacccttg aggcgatgcc gcagttaaaa aaaggggaaa tcctggaagt ggtgagcgac
    18661 tgtccacagt cgatcaataa tattccactg gatgcgcgta atcacggcta tacggtgcgg
    18721 gatattcagc aagacgggcc gaccattcgt tatttaattc agaagtaatc cctcattccc
    18781 gctgtggccc acagcgggaa catcgttgcc tctgacacat agattttacc cgccccctct
    18841 cctattcttt tcagtatgat ctcaggaggt ttctatgtgt ggacgctttg cccaatccca
    18901 aacgcgtgaa gattaccttg cgcttctcgc ggaagatatt gaacgcgata ttccctacga
    18961 tcccgaaccc attggcagat acaacgtcgc gccgggaacc aaagttcttc tgctcagtga
    19021 acgtgatgaa caccttcatc tggatccggt tttctgggga tatgctcccg gatggtggga
    19081 taaaccgccg ctgattaacg cccgcgtaga aactgccgca accagtcgca tgtttaaacc
    19141 gctctggcaa catggtcggg caatctgttt tgccgatggc tggtttgagt ggaaaaaaga
    19201 aggcgacaaa aaacagcctt tttttatcta tcgtgctgat ggacaaccta tttttatggc
    19261 cgcgataggt agcacaccat ttgagcatgg tgacgaagcc gaaggatttt taattgtcac
    19321 tgctgcagca gaccagggtc tggtagacat tcatgaccgc cgcccgctgg ttctgtcgcc
    19381 agaagctgca caggaatgga tgcggcaaga gattggcggt aaagaagcat cagaaattgc
    19441 taccaacggc tgtgtgccag caaaccagtt cacctggcac ccggtatcgc gcgcggtagg
    19501 taatgtcaaa aaccagggcg cggagttaat tcaacctgtt tgttgacatc ctccacgccc
    19561 tgaaggacga ggctccctgc tacgttcagg ctgtcgcctg aatcatttcg gtgggttcct
    19621 gcttcaacgg gcggcctgac tgcaccatct ctccacaggc aagaacggcg tgccccgccg
    19681 ctaaaatgtt acgagcgcca tttacatcgg cgttcgctgt atatccacat acctggcatc
    19741 tgaatttact ttgtgacagg cggttttctt tcgctgtatg accacagcac gcgcaacgct
    19801 ggcttgtgca cgctggcgga acagcaaaca cctgaccgcc ac
//
LOCUS       DAJTMC010000069        19694 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.069, whole genome shotgun sequence.
ACCESSION   DAJTMC010000069 DAJTMC010000000
VERSION     DAJTMC010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19694
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.069"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            51..995
                     /gene="wecH"
                     /locus_tag="OO130_002164"
     CDS             51..995
                     /gene="wecH"
                     /locus_tag="OO130_002164"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000261200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HCR5365572.1"
                     /translation="MVVMIHTTTWYVTNAHSVSPVTWDIANVLNSASRVSVPLFFMIS
                     GYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINMELALKNLLQKPVFYHLW
                     FFFAIAVIYLVSPLIQVKNVGGKMLLVLMAVIGIIANPNTVPQKIDGFEWLPINLYIN
                     GDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFIISRGTLYELQWRGNFADT
                     WYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLGIYGFHALIIHALRTRGI
                     ELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(1037..1474)
                     /locus_tag="OO130_002165"
     CDS             complement(1037..1474)
                     /locus_tag="OO130_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaB family inner membrane protein"
                     /protein_id="HCR5365573.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(1520..1861)
                     /locus_tag="OO130_002166"
     CDS             complement(1520..1861)
                     /locus_tag="OO130_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5365574.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(2026..2217)
                     /locus_tag="OO130_002167"
     CDS             complement(2026..2217)
                     /locus_tag="OO130_002167"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5365575.1"
                     /translation="MGPALGAARLAQIAANPEKSLIELLPQLPLEQSHLPDAQRYAAY
                     QPRRETFRRLYQQLLPLMA"
     gene            complement(2150..3478)
                     /gene="xylB"
                     /locus_tag="OO130_002168"
     CDS             complement(2150..3478)
                     /gene="xylB"
                     /locus_tag="OO130_002168"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HCR5365576.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWVAKLTGLCNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGCGASTGRSKAGADCGESREIAH"
     gene            complement(3595..4872)
                     /gene="xylA"
                     /locus_tag="OO130_002169"
     CDS             complement(3595..4872)
                     /gene="xylA"
                     /locus_tag="OO130_002169"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HCR5365577.1"
                     /translation="MQAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRF
                     AACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVSFYCFHD
                     VDVSPEGASLKEYINNFAQMVDVLAAKQEESGVKLLWGTANCFTNPRYGAGAATNPDP
                     EVFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVE
                     HKHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAG
                     HSFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTG
                     GLNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAARMIEDGELDKRIAQRYSGWNS
                     ELGQQILKGQMSLADLAKYAQEHNLSPVHQSGR"
     gene            5238..6209
                     /gene="xylF"
                     /locus_tag="OO130_002170"
     CDS             5238..6209
                     /gene="xylF"
                     /locus_tag="OO130_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5365578.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNGETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALTANNNKIDAVVASNDATAGG
                     AIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAEIAVELGN
                     GQEPKADTSLNNGLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKESEL"
     gene            6287..7738
                     /locus_tag="OO130_002171"
     CDS             6287..7738
                     /locus_tag="OO130_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter ATP-binding protein"
                     /protein_id="HCR5365579.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRCQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDGQHIGTRDAAGMSEDDIITMMVGRELTALYP
                     NEPHTTGDEILRIEHLTAWHPVNRHIKRVNDVSFSLKRGEILGIAGLVGAGRTETIQC
                     LFGVWPGQWEGKIYIDGKQVDIRNCQQAIAQGIAMVPEDRKRDGIVPVMAVGKNITLA
                     ALNKFTGGISQLDDAAEQKCILESIQQLKVKTSSPDLAIGRLSGGNQQKAILARCLLL
                     NPRILILDEPTRGIDIGAKYEIYKLINQLVQQGIAVIVISSELPEVLGLSDRVLVMHE
                     GKLKANLINHNLTQEQVMEAALRSEHHVEKQSV"
     gene            7716..8896
                     /gene="xylH"
                     /locus_tag="OO130_002172"
                     /pseudo
     CDS             7716..8896
                     /gene="xylH"
                     /locus_tag="OO130_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
     gene            8974..10152
                     /gene="xylR"
                     /locus_tag="OO130_002173"
     CDS             8974..10152
                     /gene="xylR"
                     /locus_tag="OO130_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator XylR"
                     /protein_id="HCR5365580.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDGVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLADWLQTLPPQTGIIAVTDARARHILQVCEHLHIPVPEKLCVIGI
                     DNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEEMPLQRILVPPVRVIERRS
                     TDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRSNLEKRFKEEVGETIHAMI
                     HAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKKAYDTTPKEYRDVNREVML
                     "
     gene            complement(10260..11084)
                     /locus_tag="OO130_002174"
     CDS             complement(10260..11084)
                     /locus_tag="OO130_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HCR5365581.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            11404..13434
                     /gene="malS"
                     /locus_tag="OO130_002175"
     CDS             11404..13434
                     /gene="malS"
                     /locus_tag="OO130_002175"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HCR5365582.1"
                     /translation="MKLASCFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGKYTLQIDTRSGTPT
                     LMISIQNAAEPVANLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDTSAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLINKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTGYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKVIEGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASSALREWKKTNPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ANVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            13612..14865
                     /gene="avtA"
                     /locus_tag="OO130_002176"
     CDS             13612..14865
                     /gene="avtA"
                     /locus_tag="OO130_002176"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HCR5365583.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPENRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNVFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(15016..15489)
                     /locus_tag="OO130_002177"
     CDS             complement(15016..15489)
                     /locus_tag="OO130_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HCR5365584.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTAVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLRQQPV"
     gene            complement(15591..16439)
                     /gene="yiaJ"
                     /locus_tag="OO130_002178"
     CDS             complement(15591..16439)
                     /gene="yiaJ"
                     /locus_tag="OO130_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HCR5365585.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DLGAIT"
     gene            16640..17638
                     /gene="yiaK"
                     /locus_tag="OO130_002179"
     CDS             16640..17638
                     /gene="yiaK"
                     /locus_tag="OO130_002179"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HCR5365586.1"
                     /translation="MKVTFEQLKAAFNRALISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            17650..18118
                     /locus_tag="OO130_002180"
                     /pseudo
     CDS             17650..18118
                     /locus_tag="OO130_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
     gene            18236..18709
                     /gene="yiaM"
                     /locus_tag="OO130_002181"
     CDS             18236..18709
                     /gene="yiaM"
                     /locus_tag="OO130_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter small
                     permease YiaM"
                     /protein_id="HCR5365587.1"
                     /translation="MKKILEVILAINLAVLSCIVFINIILRYGFQTSILSVDELSRYL
                     FVWLTFIGAIVAFMDNAHVQVTFLVEKLSPAWQRRVALVTHSLILFICGALAWGATLK
                     TIQDWSDYSPILGLPIGLMYAACLPTSLVIAFFELRHLYQLITRSNSLTSPPQGA"
     gene            18712..>19694
                     /locus_tag="OO130_002182"
     CDS             18712..>19694
                     /locus_tag="OO130_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HCR5365588.1"
                     /translation="MAVLIFLGCLLGGIAIGLPIAWALLLCGAALMFWLDMFDVQIMA
                     QTLVNGADSFSLLAIPFFVLAGEIMNAGGLSKRIVDLPMKLVGHKPGGLGYVGVLAAM
                     IMASLSGSAVADTAAVAALLVPMMRSANYPVNRAAGLIASGGIIAPIIPPSIPFIIFG
                     VSSGLSISKLFMAGIAPDMMMGATLMLTWWWQASRLNLPRQQKATMQEIWHSFVSGIW
                     ALFLPVIIIGGFRSGLFTPTEAGAVAAFYALFVATVIYREMTFATLWHVLIGAAKTTS
                     VVMFLVASAQVSTWLITIAELPMMVSDLLQPLVDSPRLLFIVIMVAILIVG"
BASE COUNT         4966 a         4933 c         4789 g         5006 t
ORIGIN      
        1 tgcagcccaa aatttactgg attgataacc tgcgagggat agcgtgttta atggtggtga
       61 tgattcacac cactacctgg tatgtgacca atgctcatag tgttagcccc gtcacatggg
      121 atatcgccaa tgttctgaat tctgcctctc gtgtcagcgt gccgctattt ttcatgattt
      181 ccggctatct cttttttggc gaacgcagcg cccagccgcg ccatttcttg cgtatcggct
      241 tatgtctgat tttttatagc gcaatcgcac tgctctacat tgcgctgttt acctccatca
      301 atatggagtt agcgctgaaa aacctgctgc aaaagccagt gttttaccac ttgtggtttt
      361 tcttcgcgat tgcggtgatt tatctggttt caccgctgat tcaggtgaag aacgtcggcg
      421 gaaaaatgtt gctggtgcta atggcggtga ttggcattat cgctaaccca aacacagtgc
      481 cgcagaaaat tgacggtttt gaatggctgc caattaactt atatatcaat ggcgatactt
      541 tttactacat tctgtatggc atgttgggcc gcgctatagg gatgatggac acacagcata
      601 aagcactgtc gtgggtgagc gccgcgctgt ttgcgacggg ggtttttatt atctctcgcg
      661 ggacattata tgaattgcag tggcgcggaa attttgccga tacctggtat ctttactgtg
      721 ggccgatggt ttttatctgc gcaatcgcgc tattgactct ggttaaaaac acgctggata
      781 cgcgtaccat tcgcggactt ggcttaatct cccgccattc attgggtata tacggattcc
      841 acgccttgat tatccatgcg ctgcgcaccc ggggaattga gcttaaaaat tggccaatac
      901 tggatattat ttggatcttt tgcgcgacgt tggcagcgag tttgttactt tctatgctgg
      961 tacaacgaat cgacagaaac agattagtga gttaagtaaa agcccgttca cattggactg
     1021 accgggctta cgtgaattat tcgctgtatt cttcctgggt aacctgtgtt tcttttggcg
     1081 ggtttattcc ggcatcacga atattcttct gcaccgctac tgcaccaaac aagcttaaga
     1141 agaaagccag tccataaaaa cctttttcac tgagtaataa tgtcgcattc cacagaccta
     1201 ccatcagtaa tgcaacagag ataataaaga cagtcaggca ggtcatataa taaatggaag
     1261 tggtcggtat gccttcatac ttgtcccgaa cggtcttttg ataagacgca gcagaaaaca
     1321 gtcctaacac cagtacggca aaataatacc ctttttcatt taactgcatc tctgcattcc
     1381 atagccctaa cagataggta acgataccac caacgagagc tatccatgac acaatactaa
     1441 aggccggtga ataggttgat attttgttgt ccatcacagt attcctttca ttcctgaatg
     1501 tgtaagagct ttatgttgct cataccgagc gaaacaaacg catttgacca aacaagcaga
     1561 caaaaaaggc gatggggtag atcaccattt cataagcatt gataggtgca tttaaaacac
     1621 cgataaacaa gagaccgata gtgattaacg caactaactg gcagatatgg gtaaaaaaat
     1681 tatccagttg cccgagtttt tctgcgcgaa gatatgcata gttaccaaaa gttgctgtca
     1741 ttaacacgcc aagaaaatag ccttttccac ttagcaatgg gcatgagatc catagcccaa
     1801 ccagataaac cagcgccccg acaacaaata atagttttgc cacagttttt gaggttttca
     1861 taacgatctc catatctacc agcgatacat tacgagtaac caacgaaaga caaaactgaa
     1921 aaatgccatt aacaaatgat tttcagaata aattcatact aaatattaat tagtgagata
     1981 tatagatgtg aattatcccc cacccggtca ggcaggggat aacgtttacg ccattaatgg
     2041 cagaagttgc tgatagaggc gacggaacgt ttcacgtcgt ggctgataag cggcataacg
     2101 ctgcgcatct ggtagatgcg actgttctaa cggtagctgc ggcaacaact caatgagcga
     2161 tttctctgga ttcgccgcaa tctgcgccag ccttgctgcg cccagtgctg gccccacatc
     2221 cccccgtacg gtaatcgagc tgctgaccgc tgatatccgc cagcatctga cgccagtact
     2281 cactacgcgc cccgccacca atcaacgtaa cactttgcgg tttaatgccg caggcatgca
     2341 cgacatccat gccatctgcc agcgcataac ccacgccttc cagcactgct cgcgccagtt
     2401 cattggggcc atgttgatga gtcaaaccaa agaaaacccc cttcgcctgg ggattattgt
     2461 gtggtgtacg ctcgccggaa agataaggca gaaaccaaac tggctcggca ctttcatcag
     2521 cctgttgagc tgcagcgatt aaagctggga cattgcacag gccggttaat ttcgcgaccc
     2581 aatccagaca cgatgctgca ctcagcatta cagacattaa atgccaacgc tgcggtagcg
     2641 catggcaaaa gctgtgtaca gcgctttctg gcttgcttaa gaacccttcg ctgacagcaa
     2701 aatagacccc cgacgtcccc agcgataaca ttgcctgatt agcatcaacc attcccacac
     2761 caactgcacc agctgcattg tcgccaccgc ctgcgacaac tggcaccgtc gccatacccc
     2821 acgctttcgc aacttcaggt aacaaagcac cagtaatttc gctgccttcg tataatgcgg
     2881 gcatctggtc acgagataag tcgcaagcct gcagcatgac gtcactccag tcacgctttg
     2941 cgacatccag ccacatggtg ccagctgcgt cagacatatc gctggcaaac tcccccgtca
     3001 tacgcagacg caagtaatct ttcggtaata atactttgtc gatttgacgg aatatctccg
     3061 gctcatgccg ctgaacccat agcaatttag gcgcagtaaa tccgggcatc atcaggttgc
     3121 cggtaatcac tcgtgattgc ggaactctcg cttccagcaa agtgcactct tgcgcacagc
     3181 gcccgtcgtt ccacaaaatg gcagggcgca atacccgttg ttgagcatcc agtaaggttg
     3241 ctccatgcat ctggccggca atacccaatg ctttaacgtc ctgcagagaa tgctgatcgc
     3301 ccagagcttt cattgcgcga tcagttgcct gccaccactg ttccgggtct tgttccgacc
     3361 agagtggatg cgggcgcgaa acggtcagct tttccgtttg cgaagcaacc acctcaccct
     3421 gctcgttgag caaaataact tttacgcccg aggtgccaag atctatcccg atatacatat
     3481 cgatcgttcc ttaaaaaaat gcccggtatc gctaccgata accgggccaa cggactgcac
     3541 agttagccgt tatttgtcga acagataatg atttaccaga ttttccagtt gctcctagcg
     3601 accactctga tgcaccggag acaaattatg ttcctgagca tatttggcta aatctgccag
     3661 tgacatttgg cctttcagga tttgctggcc caattcgcta ttccagccgg aataacgctg
     3721 cgcgatgcgt ttatccagct cgccatcttc aatcatgcgc gctgcaattt tcagcgccag
     3781 tgccatcgta tccatcgcgc cgatatgacc gtaaaacaga tcatatttat cagtactttg
     3841 acgacgtact ttggcatcga agttcagacc accggtggtg aaaccgcctg ctttgagaat
     3901 ttcatacatc accagcgcat tctcttccac actgttcggg aactggtcgg tgtcccagcc
     3961 cagttgcgca tcgccacggt tggcgtcgac agaaccgaac aggccaagcg caatggcggt
     4021 ggctatttca tgatggaaag agtgacctgc cagcgtcgcg tggttagctt caatgttcag
     4081 tttaatctct ttttccagac caaactgttt caggaagcca tagaccgtcg cggcatcgta
     4141 atcatattga tgtttagtcg gttcttgcgg tttcggttcg ataagcaacg tgccctggaa
     4201 gccgatttta tgtttatgct caaccaccat ctgcataaag cggcccagtt gttcacgctc
     4261 ctgacgcaag tcggtattta acagcgtttc gtaaccttca cgaccacccc acaggacata
     4321 gttttcaccg cccaatttat gggttgcttc catcgctgta acaacttgcg ttgccgccca
     4381 gctgaagact tcaggatctg ggttcgtcgc cgcacccgcg ccatagcgag ggtttgtaaa
     4441 gcagttagcg gttccccaca gcagcttcac gccgctctct tcttgcttgg ctgccaggac
     4501 atcaaccatt tgcgcaaaat tattgatgta ctcttttaac gacgcgccct caggggaaac
     4561 atccacatcg tggaagcaat aaaatgacac atgtaacttg tggaaaaact caaatgcgac
     4621 atctgcttta cgcttcgcca acgccagtgc ctcaccaggc tgctgccacg gacgattaaa
     4681 cgcccccaca ccaaacatgt ccgccccgtt ccagcagaag gtgtgccagt aacaggcggc
     4741 aaaacgcaag tgctcttcca tacgcttacc caacaccagt tcgtcgggat tgtagtgacg
     4801 gaatgctaac gggtttgagg attttgagcc ttcataacga acgcgatcga gctggtcaaa
     4861 ataggcttgc atattgaact ccataatcag gtaatgccgc gggtgatgga tgatgtcgta
     4921 atattgggca ctccctttca gttgctcaat tatgttattt cacactgcta ttgagataat
     4981 tcacaagtgt gcgctcgctc gcaaaataaa atgggatgat gaaactgggt aattcctcga
     5041 agataaaaat gcaataagta caattgcgca acaaaagtaa gatctcggtc ataaatcaag
     5101 aaataaacca aaaatcgtaa tcgaaagata aaaatctgta attgttttcc cctgtttagt
     5161 tgctaaaaat tggttacgtt tatcgcggtg attgttactt attaaagctg tcctctaact
     5221 acagaaggcc ctacaccatg aaaataaaga acattctact caccctttgc acctcacttc
     5281 tgcttaccaa cgttgcggca cacgccaaag aagtcaaaat aggtatggcg attgatgatc
     5341 tacgtcttga acgctggcaa aaagatcgag atatttttgt gaaaaaggct gaatctctcg
     5401 gtgcgaaagt atttgtacag tctgcaaatg gcaatggaga aacacaaatg tcgcagattg
     5461 aaaacatgat taaccggggt gtcgatgttc ttgtcattat tccatataac ggccaggtat
     5521 taagtaacgt tgtaaaagaa gccaaacaag aaggtattaa agtattagct tacgaccgta
     5581 tgattaacga tgcggatatc gatttttata tttctttcga taacgaaaaa gtcggtgaac
     5641 tgcaggcaaa agccctggtc gatattgttc cgcaaggtaa ttacttcctg atgggcggct
     5701 cgccggtaga taacaacgcc aagctgttcc gcgccggaca aatgaaagtg ttaaaacctt
     5761 acgttgattc cggaaaaatt aaagtcgttg gtgaccaatg ggttgatggc tggttaccgg
     5821 aaaacgcgct aaccgccaat aataacaaaa ttgatgctgt agttgcctca aacgatgcca
     5881 ccgcaggtgg ggcaattcag gcattaagcg cgcaaggttt atcagggaaa gtagcaattt
     5941 ccggccagga tgcggatctc gcaggtatta aacgtattgc tgccggtacg caaactatga
     6001 cggtgtataa acctattaca ttgctggcaa atactgccgc agaaattgcc gttgaattgg
     6061 gcaatggtca ggagccaaaa gcagatacct cactgaataa tggcctgaaa gatgtcccct
     6121 cccgcctcct gacaccgatc gatgtgaata aaaacaacat caaagatacg gtaattaaag
     6181 acggattcca caaagagagc gagctgtaag cgttacgccc cggcgcggag cgggggcgtg
     6241 atttctctcc atgccgcgtg aatgaattgg cttaggtgga gtcgttatgc cttatctact
     6301 tgaaatgaag aacattacca aaaccttcgg cagtgtgaag gcgattgata acgtcagctt
     6361 gcggttgaat gctggcgaaa tcgtctcact ctgtggggaa aatgggtctg gtaaatcaac
     6421 gctgatgaaa gtgctgtgtg gtatttatcc ccatggctcc tacgaaggcg aaattatttt
     6481 tgcgggagaa gagattcagg cgagtcacat ccgcgatacc gaacgcaaag gtatcgccat
     6541 cattcaccag gaattggccc tggtgaaaga attgaccgtg ctggaaaata tcttcctggg
     6601 taacgaaata acccacaatg gcattatgga ttatgacctg atgacgctac gctgtcagaa
     6661 gctgctcgca caggtcagtt tatccatttc acctgatacc cgcgttggcg atttagggct
     6721 tgggcaacaa caactggttg aaattgccaa ggcacttaat aaacaggtgc gcttgttaat
     6781 tctcgatgaa ccgacagcct cattaactga gcaggaaacg tcggttttac tggatattat
     6841 tcgcgacggt cagcacattg gtacgcgtga tgctgccgga atgagtgaag acgatattat
     6901 caccatgatg gtcgggcgag agttaaccgc gctttaccct aatgaaccac ataccaccgg
     6961 agatgaaata ttacgtattg aacatctgac ggcatggcat ccggttaatc gtcatattaa
     7021 acgagttaat gatgtctcgt tttccctgaa acgtggcgaa atactgggta ttgccggact
     7081 cgttggtgcc ggacgtaccg agaccattca gtgcctgttt ggcgtgtggc ccggacaatg
     7141 ggaaggaaaa atttatattg atggcaaaca ggtagatatt cgtaactgtc agcaagccat
     7201 cgcccagggg attgcgatgg tacccgaaga cagaaagcgc gacggcatcg ttccggtaat
     7261 ggcggttggt aaaaatatta ccctcgccgc actcaataaa tttaccggtg gcattagcca
     7321 gcttgatgac gcggcagagc aaaaatgtat tctggaatca atccagcaac tcaaagttaa
     7381 aacgtcgtcc cccgatcttg ctattggacg tttgagcggc ggcaatcagc aaaaagcgat
     7441 cctcgcccgc tgtctgttac tcaacccgcg cattctcatt cttgatgaac ctaccagggg
     7501 tattgatatt ggcgcgaaat atgagatcta caaattaatt aaccaactcg tccagcaggg
     7561 tattgccgtt attgtcatct cttccgaatt acctgaagtg ctcggcctta gcgatcgtgt
     7621 actggtgatg catgaaggga aactaaaagc caacctgata aatcataacc tgactcagga
     7681 gcaggtgatg gaagccgcat tgaggagcga acatcatgtc gaaaagcaat ccgtctgaag
     7741 tgaaattggc cgtaccgaca tccggtggct tttccgggct gaaatcactg aatttgcagg
     7801 tcttcgtgat gattgcagct atcatcgcaa tcatgctgtt ctttacctgg accaccgatg
     7861 gtgcctactt aagcgcccgt aacgtctcca acctgttacg ccagaccgcg attaccggca
     7921 tcctcgcggt aggaatggtg ttcgtcataa tttctgctga aatcgacctt tccgtcggct
     7981 caatgatggg gctattaggt ggcgtcgcgg cgatttgtga cgtctggtta ggctggcctt
     8041 tgccacttac catcattgtg acgttggttc tgggactgct tctcggtgcg tggaacggat
     8101 ggtgggtcgc gtaccgtaaa gtcccttcat ttattgtcac cctcgcgggc atgttggcat
     8161 ttcgcggcat actcattggc atcaccaacg gcacgactgt atcccccacc agcgccgcga
     8221 tgtcacaaat tgggcaaagc tatctccccg ccagtaccgg cttcatcatt ggccgcttgg
     8281 cttaatggct tttgttggtt ggcaatggcg cggaagaatg cgccgtcagg ctttgggttt
     8341 acagtctccg gcctctaccg cagtagtcgg tcgccaggct ttaaccgcta tcatcgtatt
     8401 aggcgcaatc tggctgttga atgattaccg tggcgttccc actcctgttc tgctgctgac
     8461 gttgctgtta ctcggcggaa tgtttatggc aacgcggacg gcatttggac gacgcattta
     8521 tgccatcggc ggcaatctgg aagcagcacg tctctccggg attaacgttg aacgcaccaa
     8581 acttgccgtg ttcgcgatta acggattaat ggtagccatc gccggattaa tccttagttc
     8641 tcgacttggc gctggttcac cttctgcggg aaatatcgcc gaactggacg ctattgcagc
     8701 atgcgtgatt ggcggcacca gcctggctgg cggtgtggga agcgttgccg gagcagtaat
     8761 gggggcattt atcatggctt cactggataa cggcatgagt atgatggatg taccgacctt
     8821 ctggcagtat atcgttaaag gtgcgattct gttgctggca gtatggatgg actccgcaac
     8881 caaacgccgt tcttgatttt gataaaaatt ttctcaaagc cggttacgta ttaccggttt
     8941 tgagtttttg catgattcag caggaaaaga accatgttta ctaaacgtca ccgcatcaca
     9001 ttactgttca atgccaataa agcctatgac cggcaggtag tagaaggcgt aggggaatat
     9061 ttacaggcgt cacaatcaga atgggatatt ttcattgaag aagatttccg cgcccgcatt
     9121 gataaaatca aggactggtt aggagatggc gtcattgccg acttcgacga caaacaaatc
     9181 gagcaagcgc tggctgatgt cgacgtcccc attgttgggg ttggcggctc gtatcacctt
     9241 gcagaaagtt acccacccgt tcattacatt gccaccgata actatgcgct ggttgaaagc
     9301 gcatttttgc atttaaaaga gaaaggcgtt aaccgctttg ctttttatgg tcttccggaa
     9361 tcaagcggca aacgttgggc cactgaacgc gaatatgcat ttcgtcagct tgtcgccgaa
     9421 gaaaagtatc gcggagtggt ttatcagggg ttagaaaccg cgccagagaa ctggcaacac
     9481 gcgcaaaatc ggctggcaga ctggctacaa acgctgccac cgcaaaccgg gattattgcc
     9541 gttactgacg cccgggcgcg gcatattctg caagtatgtg aacatctaca cattcccgta
     9601 ccggaaaaat tatgcgtgat tggcatcgac aacgaagaac tgacccgcta tctgtcgcgt
     9661 gtcgcccttt cttcggtcgc tcagggcgcg cggcaaatgg gctatcaggc ggcaaaactg
     9721 ttgcatcgat tattagataa agaagaaatg ccgctacagc gaattttggt cccaccagtt
     9781 cgcgtcattg aacggcgctc aacagattat cgctcgctga ccgatcccgc cgttattcag
     9841 gccatgcatt acattcgtaa tcacgcctgt aaagggatta aagtggatca ggtactggat
     9901 gcggtcggga tctcgcgctc caatcttgag aagcgtttta aagaagaggt gggtgaaacc
     9961 atccatgcca tgattcatgc cgagaagctg gagaaagcgc gcagtctgct gatttcaacc
    10021 accttgtcga tcaatgagat atcgcaaatg tgcggttatc catcgctgca atatttctac
    10081 tctgttttta aaaaagcata tgacacaacg ccaaaagagt atcgcgatgt aaatcgtgag
    10141 gtcatgttgt agcgatgatg agaattgtcg gcgtcacatc agggaactca gcacagccac
    10201 ctgatgcgag caaaagcctt atccgaccga caaatcagat aaggctcaag gaaatgcaat
    10261 tacatatgcg ccgcgattaa ccgttggtta tcctggtaca ttgcgaacag gtagttatta
    10321 taactcttcc ctttggtcga gtagcccttc agcttgtgaa tcatcgctgt ggcagtcact
    10381 tcctgatccg ctttacgcag ctgcgcacgc gatttacgga acgaagagta agccgggtgc
    10441 gtattcaggt tagtgacata ggcgctcacc gattctttga cagaactaaa ctgtgagtac
    10501 cctttcactt tacccggcgc attggtacaa cgtcctttca tgcatttcat gccgaacaga
    10561 ttgttattgt tgcgcgccag cttcgacgtt ccccaaccgc tttctgctgc agccatcgtc
    10621 gccaccatac tggtagggat aatgtctacg cgttcaagca aggtattcca cgggattttt
    10681 cgcgtattac cagaccactt caccttgtag cgtttggcga tgtctttcag acgcgcacgc
    10741 tcagcaggtg accattggcc ctgatactgt tttgaaatca gccagttacg ttctgcagta
    10801 atggctgcat tttggctggt aatgtaaggc attacggtcc ggagaaacgc ctttttcctt
    10861 ggtgttccgg aagggtattt tcgcaaatca ggaagtgaac tactctttgc actattgcga
    10921 gaatactctt gtttactgct tacctgttta ttactagctt tagttaagtg ggacttttga
    10981 ctcgctgttg ttgtgtgcgt cttcgctaac acctcactcg aaaacaccag agtgagtaac
    11041 ataagaatca tcgccccata tcgtcgtatg ggagtcaaaa tcatcaggtc tcctggtcgg
    11101 atttaatcat tccaacacct tatatttttc acaaatttga gagttgaatc tcaaatcata
    11161 tcaaaaatag ctgtcaagag caccccaagg aatagtccaa atctgaaact atgtcacgag
    11221 ttaacgattc agattggcgc taaatcgcag aaaatgtggg gtttatcgca aaattcagcc
    11281 gttttttgcg cgcgatcgct cacccttgct tctcatcctg tggacttacc gctcagggat
    11341 gagttttgtc tggcttatcg ctggcaaact gtctgaaatc gcagcaataa ggactcatcc
    11401 accatgaaac tcgcctcctg ttttctgaca ctccttcctg gcttcgccgt tgccgccagc
    11461 tggacttctc cggggttccc tgcctttagc gaacagggaa cgggaacatt tgtcagccac
    11521 gcgcagttgc ccaaaggtac gcgtccactc acgctaaatt ttgaccaaca gtgctggcag
    11581 cctgcagatg cgataaaact caatcagatg ctttccctgc aaccttgtag caacacgccg
    11641 cctcaatggc gattgttcag ggacggcaaa tatacgctgc aaatagacac ccgctccggt
    11701 acgccaacat tgatgatttc catccagaac gccgccgaac cggtagcaaa cctggtccgt
    11761 gaatgcccga aatgggatgg attaccgctc acgctggatg tcagcgccac tttcccggaa
    11821 ggagccgccg tacgggatta ttacagccag caaattgcga tagtgaagaa cggtcaaata
    11881 acgttacaac ccgctgctac cagcaacggt ttactcctgc tggaacgggc agaaactgac
    11941 acatccgccc ctttcgactg gcataacgcc acggtttact ttgtgctgac agatcgtttc
    12001 gaaaacggcg atcccagtaa tgaccagagt tacggacgtc ataaagacgg tatggcggaa
    12061 attggcactt ttcacggcgg cgatttacgc ggcctgatca acaaactgga ttacctccag
    12121 cagttgggcg ttaatgcttt atggataagc gccccatttg agcaaattca cggctgggtc
    12181 ggcggcggta caaaaggcga tttcccgcat tatgcctacc acggttatta cacacaggac
    12241 tggacgaatc ttgatgccaa tatgggcaac gaagccgatc tacggacgct ggttgatagc
    12301 gcacatcagc gcggtattcg tattctcttt gatgtcgtga tgaaccacac cggctatgcc
    12361 acgctggcgg atatgcagga gtatcagttt ggcgcgttat atctttctgg tgacgaagtg
    12421 aaaaaaacgc tgggtgaacg ctggagcgac tggaaacctg ccgccgggca aacctggcat
    12481 agctttaacg attacattaa tttcagcgac aaaacaggct gggataaatg gtggggaaaa
    12541 aactggatca gaacggatat cggcgattac gacaatcctg gattcgacga tctcactatg
    12601 tcgctggcct ttttgccgga tatcaaaacc gaatcaacta ccgcttctgg tctgccggtg
    12661 ttctataaaa acaaaacgga tactcacgct aaagtcatcg aaggctttac acctcgcgat
    12721 tacttaaccc actggttaag tcagtgggtc cgcgactatg ggattgatgg ttttcgggtc
    12781 gataccgcca aacatgttga gttgcccgcc tggcagcaac tgaaaaccga agccagctcc
    12841 gcgcttcgcg aatggaaaaa aactaacccc gacaaagcat tagatgacaa acctttctgg
    12901 atgaccggtg aagcctgggg ccacggcgtg atgcaaagtg actactatcg ccacggcttc
    12961 gatgcgatga tcaatttcga ttatcaggag caggcggcga aagcagtcga ttgtctggcg
    13021 cagatggata cgacctggca gcaaatggcg gagaaattgc agggtttcaa cgtgttgagc
    13081 tacctctcgt cgcatgatac ccgtctgttc cgtgaagggg gcgacaaagc agcagagtta
    13141 ttactattag cgccaggcgc ggtacaaatc ttttatggcg atgaatcctc gcgtccgttc
    13201 ggtcctacag gttctgatcc gctgcaaggt acacgttcgg atatgaactg gcaggatgtt
    13261 agcggtaaat ctgccgccaa cgtcgcgcac tggcagaaaa tcagccagtt ccgcgcccgc
    13321 catcccgcaa ttggcgcggg caaacaaacg acactttcgc tgaagcaggg ctacggcttt
    13381 gttcgtgagc atggcgacga taaagtgctg gtcatctggg ctgggcaaca gtgacttttc
    13441 cggcttcccg ttcatcagta cctcgggaag ccgccaacca ggataaaatg tcagccctaa
    13501 tcagcgttgc aggataaagc accgctcact cttcaacaga ccgatttgca ccccagcaaa
    13561 tgtagcgtta ttgttacctt ccttgctaca gagttcgaca gatatcccgc tatgacattc
    13621 tccctttttg gtgacaaatt tacccgccac tccggcatta cgctgctgat ggaagatctg
    13681 aacgacggtt tacgcacgcc tggcgcgatt atgctcggcg gcggtaatcc ggcgcagatc
    13741 ccggaaatgc aggactactt ccagacgcta ctgaccgaca tgctggaaag tggcaaagcg
    13801 actgatgcac tgtgtaacta cgacggtcca caggggaaaa cggagctact cacactgctt
    13861 gccggaatgc tgcgcgagaa gttgggttgg gatatcgaac cacagaatat tgcactaaca
    13921 aacggcagcc agagcgcgtt tttctactta tttaacctgt ttgccggacg ccgtgccgat
    13981 ggtcgggtca aaaaagtgct gttcccgctg gcaccggaat acattggcta cgctgacgcc
    14041 ggactggaag aagatctgtt tgtctctgcg cgtccgaata ttgaactgct gccggaaggc
    14101 cagtttaaat accacgtcga ttttgagcat ctgcatattg gcgaagaaac cgggatgatt
    14161 tgcgtctccc ggccgacgaa tccaacaggc aatgtgatta ctgacgaaga gttgctgaag
    14221 cttgacgcgc tggcgaatca acacggcatt ccgctggtga ttgataacgc ttatggcgtc
    14281 ccgttcccgg gtatcatctt cagtgaagcg cgcccgctat ggaatccgaa tatcgtgctg
    14341 tgcatgagtc tttccaagct ggggctgcct ggctcccgct gcggcattat catcgccaat
    14401 gaaaaaatca tcactgccat caccaatatg aacggcatta tcagcctggc acctggcggt
    14461 attggtccgg cgatgatgtg tgaaatgatt aagcgtaacg atctgctgcg cctgtctgaa
    14521 acagtcatca aaccgtttta ctaccagcgt gttcaggaaa ctatcgccat cattcgccgc
    14581 tatttaccgg aaaatcgctg cctgattcat aaaccggaag gagccatttt cctctggcta
    14641 tggtttaagg atttgcccat tacgaccgag cagctctatc agcgcctgaa agcacgcggc
    14701 gtgctgatgg tgccggggca caacgtcttc ccgggactgg ataaaccgtg gccgcatacg
    14761 catcaatgta tgcgcatgaa ctacgtacca gagccggaga aaattgaggc gggggtgaag
    14821 attctggcgg aagagataga aagagcctgg gctgaaagtc actaaaaatt gcctgatgcg
    14881 ctacgcttat caggcctaca tgatttctgc aatatattga atttggaaga atttgtaggc
    14941 cggataaggc gtttacgccg catctggcat aaacaaagcg cactattccg gcgttgagaa
    15001 acgccggaaa acgtttcaaa caggctgctg ccgtagccga tgccgctgca ctttctcgac
    15061 atccatgcac tgcaacgcct ttgtcgggca ggcttcaaca caggccggcc ccgtctcccg
    15121 atgccagcac agatcgcact taatcgccct cgcctttttg cgcgaagaaa cgacctccat
    15181 cgcaccaaac gggcaagcca gcatacagct tttacagcca atgcaacgtg tttgttcaac
    15241 gaaaatatgc ccatgttcgc ggcttatcgc gtcaacaggg cagacattcg cgcacggtgc
    15301 atcttcacac tgatgacagg ctactgccgt ggtccagctg tggtctttaa tgacacgaat
    15361 acgggaaata aactcgtctg gtgacaacgc agcgcaatcc tgattctcct gatgcgacac
    15421 tgcgcaagcc acttcacagg tacggcaacc gatacatttc gtcgcatccg caataataaa
    15481 ccggttcatg atcactcctt tgcactaaaa cagcagagtg ccagagagcg cgtccctact
    15541 gctttgatcc aacgaaggaa atgttaaagt tttggcactt gtccaaagcg ttatgtgatc
    15601 gcgcccaggt catccctgac agtaaatccc agttcattag aaatagcctg cgcggtttca
    15661 cgcagtggtt tcaggagatt tttctctccc acctgtttca gacgtgatgt cgaaagcgaa
    15721 atcgacacgg cgtacggcac ccgcccatga atatcaaaca ccggaacagc aatacaggag
    15781 acgccgagtt cgttttcttc tctgtccatc gccgctccgc tttcacgaat gtgcgccagt
    15841 tcgtcgaaca tcgcgggcag ctcggtaatg gtattgcggg ttaacggctg gatctcatgc
    15901 tgatggtttt cccagtatga cttcacgtag tccgggtgac caaacgccat gtagatcttg
    15961 cccattgcgg aacagtagag cggcatatgc tggccaatat aggcacgggt tcgcagcatc
    16021 ccggttgtgg gttccagctt ataaatcaaa atagcgtgat cgtcttcgcg gctggagaag
    16081 ttaatggttt caccagtggc gatgttcagt gcctcaagat gcggagcggc gatatgaatg
    16141 atattcagcg aagacagcgc cttctgcccg acggcaataa atttggtagt caggcggtaa
    16201 ctccctgcgg cgggcgcggt ggtcacatag ccgcaggact gtaatccctg caataagcga
    16261 tggacggtac tcttatttaa accagccagc tccgaaagat gcgccaacgg acaaccgttt
    16321 ggatagttgc tcaaaatctc aatcagcatc aacccgcgaa acagactctg gcttccggct
    16381 ggacgctctt tttcctgcgc catctcgttc tctttttttc ccatcacttc tttccccatt
    16441 ttgtcgcgtc ctgatggtag cgcaaagtgt gccgtagttc acgatctcga cagataattt
    16501 ataaccactt gatttttatg tcttttgaaa ttcatcaatc agattgcttt gttaaaaagt
    16561 gatcgatata tttgaaatca agtttcgcat attgaaattt taagccaaaa aagcgatcaa
    16621 aaaaacaagg aagcctggga tgaaagtgac atttgagcag ttaaaagcag cctttaatcg
    16681 ggccttaatt tcacgcggcg ttgacagcga aacggctgac gcctgtgcag agatgttcgc
    16741 ccgcaccacc gaatccggcg tttattctca cggcgttaat cgtttccctc gtttcattca
    16801 acaactggaa aacggcgata tcattcctga tgcccaaccc aaacgtataa ccagcctcgg
    16861 cgcaattgaa cagtgggatg cccagcgttc gatcggcaac ctgacggcga aaaagatgat
    16921 ggatcgcgcc attgaactgg ctgccgatca cggtattggt ctggtggcac ttcgtaatgc
    16981 caaccactgg atgcgcggcg gcagctacgg ctggcaggcg gcggaaaaag gctatattgg
    17041 catttgctgg accaactcca tcgccgtaat gccgccgtgg ggcgcaaaag agtgtcgcat
    17101 tggcactaac ccgctgatcg tcgccattcc ttccacgccg atcaccatgg tcgatatgtc
    17161 gatgtcgatg ttctcttacg gcatgttaga agttaaccgc ctggcgggcc gtcagctccc
    17221 ggtcgatggt ggctttgatg atgagggcaa tttgaccaaa gaacctggcg ttatcgagaa
    17281 gaatcgccgc attttgccga tgggctactg gaaaggttct ggcatgtcga ttgtgctgga
    17341 tatgatcgct actctccttt ccgacggcgc atcggttgcc gaagtcaccc aggacaacag
    17401 cgacgaatac ggcatttcac aaatttttat tgccattgaa gtggacaagc ttatcgacgg
    17461 tcccacccgc gatgccaagc tgcaacgcat catggattac gttactaccg ctgaacgcgc
    17521 tgacgaaaac caggccatcc gcttacccgg ccacgaattt actaccctgc tggccgaaaa
    17581 ccgccgtaac ggcatcactg ttgatgacag cgtgtgggcc aaaatccagg cgttatgagg
    17641 agataagtca tgatttttgg acatatcgcg cagcccaatc cgtgccgttt gcccgccgcc
    17701 attgaaaagg cgctcgattt tctgcgcgct accaacttca acgtcctgga gccgggcgtt
    17761 gtcgaaatcg acggcaagaa tatttatgcg caaattatcg atttaaccac acgcgaagcg
    17821 gtggaaaacc gtccggaagt gcatcgtcgg tatatcgata ttcagtttct ggcgtggggt
    17881 caagagaaaa tcggcattgc tattgataca ggaaataata aagtcagcga atcactatta
    17941 gagcagcgcg atattatttt ttatcatgtc agtgaacatg aatcatttat agaaatgata
    18001 ccgggcagct acgctatttt cttcccgcag gatgttcacc gaccaggttg tattctgcaa
    18061 acaggcctct gagatacgaa aaattgtggt taaagtcgca ttaacagcgc tgaattaatc
    18121 aaataatcgt ctacgccaga acgactgggt aatgttattg ctctaactat attaataatt
    18181 taggttgctg cacatgcaac ttgaattatg acagacatag cctcaaggaa tagctatgaa
    18241 aaaaatactt gaagtaatac tggcgattaa tctcgccgta ctttcctgta ttgtgtttat
    18301 aaacattatt ttaagatatg gttttcagac aagcatttta tctgtagatg aattgtcacg
    18361 ttatttattt gtctggttaa catttattgg cgcgattgtg gcttttatgg ataacgccca
    18421 cgttcaggtc acctttctgg tggagaaact ctcccccgca tggcaacgac gagtcgctct
    18481 ggttacccat tccttaatct tatttatttg tggcgcactg gcctggggcg caacactaaa
    18541 aacaattcag gactggagcg attattcacc gatcctcggt ttgcccatcg gcctgatgta
    18601 tgccgcttgt ctgcccacca gcctcgttat cgcattcttt gaactgcgtc atttatatca
    18661 actcatcacg cgtagcaatt ctctaacgtc gccaccgcaa ggagcttaat catggctgtg
    18721 ctgatttttc tgggctgtct gttgggtggt attgctatcg gtttgcctat tgcctgggca
    18781 ctgttgttgt gcggggcggc gttaatgttc tggctggaca tgtttgatgt ccagatcatg
    18841 gcacaaacgc tggtgaacgg agccgatagc ttctccctgc tggcgattcc gttctttgtg
    18901 ctggcgggtg aaatcatgaa tgcgggcggc ttgtcaaaac gcattgttga cctgccgatg
    18961 aaactggtgg ggcataaacc aggcgggctg ggctacgtcg gcgtgctggc agctatgatt
    19021 atggcgagcc tttctggttc tgccgttgcg gataccgccg ccgttgccgc tctactggtg
    19081 ccgatgatgc gcagcgccaa ctacccggtc aaccgggcgg cggggctgat tgcctctggc
    19141 ggcattatcg cgccaattat tcccccttcc attccgttta ttatcttcgg cgtttccagc
    19201 ggattatcca tcagtaagct gtttatggcg ggcattgccc ctgacatgat gatgggcgca
    19261 acgctgatgc ttacctggtg gtggcaggcc agtcgcctta atttaccccg ccagcaaaaa
    19321 gcaacgatgc aggaaatctg gcactctttt gtctccggta tctgggctct gtttcttcca
    19381 gtcatcatta ttggcgggtt tcgctccggt ctgtttaccc ctaccgaagc cggggccgtt
    19441 gctgctttct atgcgctgtt tgtcgccaca gttatttacc gtgaaatgac cttcgccaca
    19501 ctttggcatg tactcattgg cgcggcgaaa accacctcag tggttatgtt tctggtggcc
    19561 tcggcacaag tttccacctg gctgattacc attgctgaac tgccgatgat ggtttctgat
    19621 ttactgcaac cgttggtcga ttcaccgcgt ctgctgttta tcgtcattat ggtggctatc
    19681 ctgattgtcg gcat
//
LOCUS       DAJTMC010000070        19606 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.070, whole genome shotgun sequence.
ACCESSION   DAJTMC010000070 DAJTMC010000000
VERSION     DAJTMC010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19606
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.070"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(<1..184)
                     /locus_tag="OO130_002183"
     CDS             complement(<1..184)
                     /locus_tag="OO130_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5365589.1"
                     /translation="MKNIKLLGSAMLVSLALFSQNSLAKEYKIGASLLTQQHPFYIDL
                     ANAMKEEAKKDNVNLSV"
     gene            complement(220..1074)
                     /locus_tag="OO130_002184"
     CDS             complement(220..1074)
                     /locus_tag="OO130_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000275530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose bisphosphate family class II aldolase"
                     /protein_id="HCR5365590.1"
                     /translation="MYIISSKNMLKKAQSEGYAVPAFNIHNLETLQVVVESAAQLRSP
                     VMLAGTPGTYRYGGVGSLISLVQSLAREYNLPLVLHLDHHEESDDIFNKVRAGIRSVM
                     IDGSHLPFEDNIARVQEVTTFCHRFDVSVEAELGRLGGQEDDLLVDEKDSAYTSPAAA
                     AEFVQRTQIDSLAVAIGTAHGLYAHEPRLDFVRLEQIRQRVEIPLVLHGASGLSEQDV
                     QHCIRRGICKVNVATELKIAFADALKGYFYNNPAANDPRHYMQPAKAAMKEVVTRIIG
                     VCGSEGKI"
     gene            1417..2247
                     /locus_tag="OO130_002185"
     CDS             1417..2247
                     /locus_tag="OO130_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR5365591.1"
                     /translation="MKSVNVPRKSSNLLKRRLKIAEIVAAQGEIKVDDLSAQLGVSGV
                     TIRGDLSYLEQQGYLKRSFGGAIATPLSSEPEAIQPIAPLSLAQQMEIARHCARLIRD
                     RDTLFLGHGTICRKIIPLLSEVKKLRLITNDPEHALLANQFIDGEIILAGSEMLRPDT
                     ALAGKPLEQALQHFTITHSILEINHLDADGTLNINVPRLAAAWQLCFDHAQNKTVIVA
                     ADPAPSTAAIGHLHQAENVIVSRSINEQYQQQLQSADFVILYTSNECFTWSNRERLQE
                     "
     gene            2279..3214
                     /locus_tag="OO130_002186"
     CDS             2279..3214
                     /locus_tag="OO130_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001446002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCR5365592.1"
                     /translation="MGELLAEFLSRNPHQKFTQPGEFIGPFPSGAPAIFAAQVAKLSH
                     RAIFFGCVGNDDFGRLIIERLRHEGVITDGIHVMNNAVTGTAFVSYQNPQQRDFVFNI
                     PNSACGLFTAEHIDKDLLKQCNHLHIVGSSLFSFRMIDVMRKAITTIKSAGGTVSFDP
                     NIRKEMLSIPEMAQALDYLIEYTDIFIPSESELPFFARHKNLSEEQIVSDLLHGGVKH
                     VAIKRAQRGASYYKLKNGTLHAQHVTGHDIEIIDPTGAGDCFGATFITLFLSGFPAHK
                     ALQYANASGALAVMRQGPMEGISSLADIEDFLQQH"
     gene            3307..3549
                     /locus_tag="OO130_002187"
     CDS             3307..3549
                     /locus_tag="OO130_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HCR5365593.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLAAAYLPDVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            3539..4990
                     /gene="panF"
                     /locus_tag="OO130_002188"
     CDS             3539..4990
                     /gene="panF"
                     /locus_tag="OO130_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HCR5365594.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVETLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFY
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            5002..5883
                     /gene="prmA"
                     /locus_tag="OO130_002189"
     CDS             5002..5883
                     /gene="prmA"
                     /locus_tag="OO130_002189"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HCR5365595.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            6212..7177
                     /gene="dusB"
                     /locus_tag="OO130_002190"
     CDS             6212..7177
                     /gene="dusB"
                     /locus_tag="OO130_002190"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HCR5365596.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            7203..7499
                     /gene="fis"
                     /locus_tag="OO130_002191"
     CDS             7203..7499
                     /gene="fis"
                     /locus_tag="OO130_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HCR5365597.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            7585..8469
                     /gene="yhdJ"
                     /locus_tag="OO130_002192"
     CDS             7585..8469
                     /gene="yhdJ"
                     /locus_tag="OO130_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCR5365598.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKIPAESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYDPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAVSSGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            8553..8732
                     /locus_tag="OO130_002193"
     CDS             8553..8732
                     /locus_tag="OO130_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HCR5365599.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLTTETDKCRNMNSVNP
                     LKLVNCDELNFQDRM"
     gene            complement(8735..9397)
                     /gene="acrS"
                     /locus_tag="OO130_002194"
     CDS             complement(8735..9397)
                     /gene="acrS"
                     /locus_tag="OO130_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HCR5365600.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTLNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQEHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDEMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIINGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHQTNELS
                     VM"
     gene            9796..10953
                     /gene="acrE"
                     /locus_tag="OO130_002195"
     CDS             9796..10953
                     /gene="acrE"
                     /locus_tag="OO130_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HCR5365601.1"
                     /translation="MTKHARFFLLPSFILISAALIAGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     NYNSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     GTVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDESTGSITLR
                     AVFPNPQHTLLPGMYVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNDKSQVEAR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
     gene            10965..14069
                     /gene="acrF"
                     /locus_tag="OO130_002196"
     CDS             10965..14069
                     /gene="acrF"
                     /locus_tag="OO130_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
                     /protein_id="HCR5365602.1"
                     /translation="MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVS
                     VSANYPGADAQTVQDTVTQVIEQNMNGIDNLMYMSSTNDSAGSVTITLTFQSGTDPDI
                     AQVQVQNKLQLATPLLPQEVQQQGISVEKSSSSYLMVAGFVSDNPGTTQDDISDYVAS
                     NVKDTLSRLNGVGDVQLFGAQYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQ
                     LGGTPALPGQQLNASIIAQTRLKNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYN
                     VIARINGKPAAGLGIKLATGANALDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQ
                     LSIHEVVKTLFEAIMLVFLVMYLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMMEDKLPPKEATEKSMSQIQGALVGIAMVLS
                     AVFIPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATLLKPVSAEHHENK
                     GGFFGWFNTTFDHSVNHYTNSVGKILGSTGRYLLIYALIVAGMVVLFLRLPSSFLPEE
                     DQGVFLTMIQLPAGATQERTQKVLDQVTDYYLKNEKANVESVFTVNGFSFSGQAQNAG
                     MAFVSLKPWEERNGDENSAEAVIHRAKMELGKIRDGFVIPFNMPAIVELGTATGFDFE
                     LIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPNGLEDTAQFKLEVDQEKAQALSV
                     SLSDINQTISTALGGTYVNDFIDRGRVKKVYVQADAKFRMLPEDVDKLYVRSANGEMV
                     PFSAFTTSHWVYGSPRLERYNGLPSMEIQGEAAPGTSSGDAMALMENLASKLPAGIGY
                     DWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSIPVSVMLVVPLGIVGVLLA
                     ATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGKGVVEATLMAVRMRLR
                     PILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLAIFFLPVFFVVIRR
                     CFKG"
     gene            14322..14543
                     /locus_tag="OO130_002197"
     CDS             14322..14543
                     /locus_tag="OO130_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5365603.1"
                     /translation="MKRLIPVALLTALLAGCAHDSPCVPVYDDQGCLVHTNTCMKGTT
                     QDNWETAGAIAGGAAAVAGLTMGIIAMSK"
     gene            14974..16023
                     /locus_tag="OO130_002198"
     CDS             14974..16023
                     /locus_tag="OO130_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5365604.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LLGFSYADADGKFSGIDVDVDVDVDVDVCRSVAAAVFGDDTKVKYTPLTAKERFTALQ
                     SGEVDLLSRNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQ
                     AGTDTELNVADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLS
                     NPAEWIVLPEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAAN
                     PATPDMAHLLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQN
                     NLWNNGGIQYAPPVR"
     gene            16091..17261
                     /locus_tag="OO130_002199"
                     /pseudo
     CDS             16091..17261
                     /locus_tag="OO130_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
     gene            17271..18374
                     /locus_tag="OO130_002200"
     CDS             17271..18374
                     /locus_tag="OO130_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCR5365605.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     VLLIGLVSIAPMFWKILPHRGRYIAVWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            18382..19139
                     /locus_tag="OO130_002201"
                     /pseudo
     CDS             18382..19139
                     /locus_tag="OO130_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
     gene            complement(19371..19486)
                     /gene="rrf"
                     /locus_tag="OO130_002202"
     rRNA            complement(19371..19486)
                     /gene="rrf"
                     /locus_tag="OO130_002202"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(19526..19601)
                     /locus_tag="OO130_002203"
     tRNA            complement(19526..19601)
                     /locus_tag="OO130_002203"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19566..19568),aa:Thr,seq:ggt)
BASE COUNT         4813 a         4682 c         4903 g         5208 t
ORIGIN      
        1 agacacttaa attgacattg tctttttttg cctcttcttt catggcattc gcaagatcaa
       61 tatagaaagg atgttgctgt gttaataatg acgcaccaat tttatactct tttgccaacg
      121 aattttgcga gaacaatgcc aggctcacca acatagctga accaagtaat ttaatatttt
      181 tcataaacaa tcctcattgt ttttgtaggg tacaacgtct taaatttttc cttcactacc
      241 acagacacca ataatacgag ttacgacttc tttcatggcg gcctttgcgg gctgcatata
      301 gtggcgtgga tcatttgccg caggattgtt gtagaaatat ccttttaacg catcggcaaa
      361 cgcaattttc aattctgtcg cgacattcac tttacaaata ccgcggcgaa tacagtgctg
      421 aacgtcctgt tctgataagc ccgaagcacc atgcaacacc agtggaatct ccactcgctg
      481 acgaatctgt tccagccgca caaaatccag tcgtggttca tgcgcatata aaccatgtgc
      541 tgtgccgatt gctacggcca gggagtcaat ttgggtacgt tgaacaaact ctgcagctgc
      601 ggctggtgag gtataagcgg aatctttttc atccaccagc agatcgtcct cctggccgcc
      661 aagacgccct agctcggcct caacgctaac atcaaagcga tggcaaaagg tggtaacttc
      721 ttgcactcgc gcaatgttgt cttcaaatgg caaatgggag ccatcaatca tgacagaacg
      781 gatccctgcg cgaaccttgt taaaaatgtc atcactctct tcatgatggt cgagatgcag
      841 gactaacggc aggttgtact cgcgagccag cgattgtact aacgaaatta aacttcccac
      901 gccaccataa cgataagtac cgggtgttcc cgccagcata acgggagagc gaagttgtgc
      961 agcgctctca acaactacct gtaaagtttc aaggttatga atgttgaaag cggggacggc
     1021 atatccctct gactgggctt ttttcagcat gtttttgctt gaaatgatgt acatcgcagc
     1081 tcctgttatt tgtcgttcgc cttacaaaat gaagaaagag aatattcact ttcattttga
     1141 aagcaaacta gcacagcaaa agcggcaaaa agcgtgatac atgtcgaata tcatcaaaat
     1201 aacttacaaa aaaataactt aatttgtttt tcactttaca agtgaaacca ataacatctc
     1261 tgcataaata tgaataaatt catttatttt tcaatgaatt aatctatttt tgttgcgatt
     1321 tttgacccat gattttcttt tcgaattgtc gatattattg aaggataact ttcaaagaaa
     1381 aatacaaggg gctatagtgt tacgcattaa aggccaatga agagtgttaa cgtgccgaga
     1441 aaatcgtcta acctactgaa acgcagatta aaaattgcag aaattgttgc cgcccaaggt
     1501 gaaatcaaag ttgatgactt atctgcgcaa cttggtgtct ctggcgtgac gatccgcggc
     1561 gatctcagtt atctggagca acagggatat ttaaagcgat catttggcgg tgctattgcg
     1621 acaccactct cctctgaacc agaagcgata caaccgatcg cgccattgtc tctggcacaa
     1681 caaatggaaa tagcacgaca ttgcgcacgg ttaattcgcg atcgtgacac gctatttctt
     1741 ggacatggga caatctgccg taagatcatt ccattgctca gcgaagtgaa aaaactgcgt
     1801 ctgatcacca acgatcccga acatgcgctg ctggcaaatc agtttattga tggcgaaata
     1861 atactggctg gcagtgaaat gctgcgcccc gacaccgcac tggccggaaa accgcttgaa
     1921 caggcacttc agcatttcac cattacacat agcattctgg aaatcaacca tctggatgct
     1981 gatggtactt taaatatcaa cgttcccaga ctggccgctg cctggcagct ctgtttcgat
     2041 catgcccaga ataagacggt tatcgttgcc gccgatcctg cgccatcaac ggctgctatc
     2101 gggcatttgc atcaggcaga aaatgtgatc gtcagccgca gcattaacga acaatatcag
     2161 caacaattgc agtcggctga tttcgttatc ctttatacca gtaatgagtg ctttacctgg
     2221 tccaatcgcg aaaggcttca ggaatgagag gggttttgtg acaataacta tctgcgctat
     2281 gggcgaattg ctggccgagt ttttgtcccg caacccacat caaaaattca ctcagcctgg
     2341 ggagtttatc gggccatttc ccagcggtgc gccagcaatt tttgctgctc aggtggcaaa
     2401 actgtcccat cgggccatct tcttcggatg tgttggtaat gatgattttg gccgactcat
     2461 tatagagcgc ctccgtcatg aaggtgtcat taccgatggg atccatgtta tgaacaatgc
     2521 cgtcacaggt acggcgttcg tgagttatca aaatccccag cagcgcgatt tcgtctttaa
     2581 tatccctaac agcgcctgcg gcttgtttac tgctgagcac attgataagg atctgctaaa
     2641 acagtgtaac catctccata ttgtgggctc atcattgttc tcatttcgca tgatcgatgt
     2701 catgcgtaaa gcaataacga cgatcaaatc ggctggcggc accgtttctt tcgatcccaa
     2761 tattcgcaaa gagatgctga gcattcctga aatggcgcag gctctcgatt atttgattga
     2821 atatacggat attttcatcc ccagcgaaag cgaactccct ttcttcgcgc gtcacaaaaa
     2881 tctgtcagaa gaacagattg ttagcgatct tctccacggc ggcgtaaaac atgtggcgat
     2941 aaaacgcgcc cagcgtgggg ccagctatta caagcttaaa aacggtacat tacacgccca
     3001 gcatgttaca ggccacgata tcgaaattat cgatccaacg ggtgcaggcg actgctttgg
     3061 cgcaacgttt atcactcttt tcttatccgg tttcccggca cataaggcac tgcaatatgc
     3121 aaatgccagc ggcgcgctcg ccgtaatgcg gcaaggtccg atggaaggga tatcctcact
     3181 ggctgacatt gaagactttt tgcagcagca ctgaacggca attttctgtc agattatttt
     3241 ccctgcctga agagttgccg tagaattgcc cgtttctcgt atccccccat gaggaggaac
     3301 ccttccgtgg acactcgttt tgttcaggcc cataaagagg cgcgctgggc gctggggctg
     3361 acccttttgt atctggcagt ttggttagca gccgcttact tacctgacgt tgcccccggt
     3421 tttaccggct ttccgcgctg gtttgagatg gcctgcatcc tgacgccgct gctgtttatt
     3481 ggactgtgct gggcgatggt gaaatttatc tatcgcgata tcccactgga ggatgacgat
     3541 gcagcttgaa gtgattctac cgctggtcgc ctatctggtg gtggtgttcg gtatctctgt
     3601 ttatgcgatg cgtaaacgga gcaccggtac cttccttaat gagtatttcc tcggcagccg
     3661 ctctatgggc ggtattgtgc tggcgatgac gctcaccgcg acctatatca gtgcaagttc
     3721 gtttatcggc gggcctggag ctgcttataa atacggcctg ggttgggtat tgctggcgat
     3781 gattcagctt cctgcagtct ggctttcgct cggtattctc ggcaagaagt tcgcgattct
     3841 tgcgcgccgc tacaatgcag tgacgctgaa cgacatgctg tttgcccgct accagagtcg
     3901 tcttctggtg tggctggcga gtttgagttt gctggttgcg ttcgttgggg caatgaccgt
     3961 gcagtttatc ggcggtgcgc gcctgctgga aaccgcggcg ggtattcctt atgaaaccgg
     4021 gctgctgatt ttcggtatca gcattgcgtt gtataccgcc tttggcggct ttcgcgccag
     4081 cgtgctgaac gacaccatgc aagggcttgt gatgctgatt ggcaccgttg tgctgcttat
     4141 tggcgtggta catgccgcag gtggtttaag taatgcggta gagacgctgc aaaccatcga
     4201 tccgcaactg gttacgccac aaggcgctga cgatattctg tcgcctgcct ttatgacgtc
     4261 gttctgggta ctggtgtgct ttggcgtgat tggcctgccg catactgcgg tgcgctgtat
     4321 ctcttataaa gacagcaaag cggtacaccg ggggatcatc atcggtacga ttgtagtcgc
     4381 aatcctgatg ttcggtatgc acctggccgg agcgttaggt cgtgcagtga tccccgatct
     4441 caccgtaccg gatctggtga tcccgacgtt aatggtaaaa gtgctgccac cgtttgctgc
     4501 cgggatcttt ctggctgcac ctatggctgc gatcatgtcg acaattaacg cccaattgct
     4561 acaaagttcc gctacgatca ttaaagatct ctatctgaat atccgtccgg atcaaatgca
     4621 aaacgagacg cgtctgaagc ggatgtcggc ggtaattacg ttagttctcg gcgcgttgct
     4681 gctgcttgcc gcctggaagc cgccagaaat gatcatctgg ctgaatttgc tggccttcgg
     4741 tgggttggaa gccgttttcc tgtggccgct ggtgctgggt ctttactggg aacgcgccaa
     4801 cgccaaaggc gcgctaagtg cgatgatcgt tggcggcgtg ctgtatgccg tactcgcgac
     4861 gctgaatatt cagtacctgg gcttctaccc tatcgtgccc tcgttactac taagtttgct
     4921 ggctttcctg gtcggaaacc gtttcggtac atccgtcccg caagctaccg ttttgactac
     4981 tgataaataa agagttttgc catgccttgg atccaactga aactgaacac caccggcgcg
     5041 aacgctgaag atcttagcga tgcgctgatg gaagcgggtg ccgtttctat cacttttcag
     5101 gatacccacg atacgccagt atttgagccg ctgccgggcg aaacgcgcct gtggggcgac
     5161 accgatgtga ttggtctgtt cgacgctgaa accgatatga acgacgtggt ggcgattctg
     5221 gaaaaccatc cgctgctcgg cgcaggcttc gcgcataaaa tcgaacaact ggaagataaa
     5281 gactgggagc gcgaatggat ggataatttc cacccgatgc gctttggtga acgactgtgg
     5341 atctgcccaa gctggcgtga tgtgccggac gaaaacgccg tcaacgtgat gttagaccca
     5401 gggctggcgt ttggtacggg tacccatcca actacctctc tgtgcctaca atggctcgac
     5461 agcctcgatt taactggtaa aacagtcatc gactttggct gcggttccgg cattctggct
     5521 atcgcggcgc tgaaactggg tgcagcaaaa gccattggta ttgatatcga tccgcaggcg
     5581 attcaggcta gccgcgacaa cgccgaacgt aatggcgttt ctgaccgtct ggaactctac
     5641 ttaccgaaag atcagccaga agaaatgaaa gccgacgtgg tggtcgcgaa catccttgca
     5701 ggcccattac gtgaactggc accgttaatc agcgtcctgc cggtttcagg cggtttgctg
     5761 ggcctttccg gtattctggc aagccaggca gagagcgttt gtgaagctta tgccgatagc
     5821 ttcgcactgg acccggtcgt ggaaaaagaa gagtggtgcc gtattaccgg tcgtaagaat
     5881 taaccttcgc atcgccgtag ggtgacgcgg ggcgaagtgc gagcaagctc acaaaaggca
     5941 cgtaaatttg ccggttattt acgcaaattt gcgtgccaaa attttcattc ataaagaaaa
     6001 attgagaact tactcaaatt tctttgaatg taaattttag tcactatttt ctaatatgat
     6061 gatttttatg agtaattatc gcaccacgct cattttaaat gcaattcttt gatccatctc
     6121 agaggattgg tcaaagtttg gcctttcatc tcgtgcaaaa aatgcgtaat atacgccgcc
     6181 ttgcagtcac agtatggtca tttcttaact catgcgcatc ggacaatatc agctcagaaa
     6241 tcgcctgatc gcagcgccca tggctggcat tacagacaga ccttttcgga cgttgtgcta
     6301 cgagatggga gccggattga cagtatccga gatgatgtct tctaacccac aggtttggga
     6361 aagcgacaaa tctcgtttac ggatggtgca cattgatgaa cccggtattc gcaccgtgca
     6421 aattgctggt agcgatccga aagaaatggc agatgcagca cgtattaacg tggaaagcgg
     6481 tgcccagatt attgatatca atatgggttg cccggctaaa aaagtgaatc gcaagctcgc
     6541 aggttcagcc ctcttgcagt acccggatgt cgttaaatcg atccttaccg aggtcgtcaa
     6601 tgcagtggac gttcctgtta ccctgaagat tcgcactggc tgggcaccgg aacaccgtaa
     6661 ctgcgaagag attgcccaac tggctgaaga ctgtggcatt caggctctga ccattcatgg
     6721 ccgtacacgc gcctgtttgt tcaatggaga agctgagtac gacagtattc gggcagttaa
     6781 gcagaaagtt tccattccgg ttatcgcgaa tggcgacatt actgacccgc ttaaagccag
     6841 agctgtgctc gactatacag gggcggatgc cctgatgata ggccgcgcag ctcagggaag
     6901 accctggatc tttcgggaaa tccagcatta tctggacact ggggagttgc tgcccccact
     6961 gcctttggca gaggttaagc gcttgctttg cgcgcacgtt cgggaactgc atgactttta
     7021 tggtccggca aaagggtacc gaattgcacg taaacacgtt tcctggtatc tccaggaaca
     7081 cgctccaaat gaccagtttc ggcgcacatt caacgccatt gaggatgcca gcgaacagct
     7141 ggaggcgttg gaggcatact tcgaaaattt tgcgtaaaca gaaataaaga gctgacagaa
     7201 ctatgttcga acaacgcgta aattctgacg tactgaccgt ttctaccgtt aactctcagg
     7261 atcaggtaac ccaaaaaccc ctgcgtgact cggttaaaca ggcactgaag aactattttg
     7321 ctcaactgaa tggtcaggat gtgaatgacc tctatgagct ggtactggct gaagtagaac
     7381 agcccctgtt ggacatggtg atgcaataca cccgtggtaa ccagacccgt gctgcgctga
     7441 tgatgggcat caaccgtggt acgctgcgta aaaaattgaa aaaatacggc atgaactaat
     7501 tcaggttagc taaatgcttg attaaaaagg cgctactcgg catggggaag cgcctttttt
     7561 ataggtgtca caaagggagt gaccatgaga acaggatgtg aaccgacccg gtttggtaat
     7621 gaagctaaga ccattattca cggtgatgcg cttgccgaac ttaaaaagat ccccgccgaa
     7681 agtgtcgatc tgatctttgc cgacccacca tataacatcg gtaaaaattt tgatggtctg
     7741 atcgaagcct ggaaagaaga tctgtttatc gactggctgt ttgaagtgat tgcagagtgc
     7801 caccgcgttc tgaaaaagca gggcagcatg tacatcatga acagcacgga aaacatgccc
     7861 tttatcgatc tccagtgccg caagcttttt accatcaaaa gtcgcatcgt ctggtcatat
     7921 gacagttctg gagtacaggc aaaaaaacac tacggctcca tgtacgaccc catcctgatg
     7981 atggtgaaag acgcaaagaa ctacacattc aacggtgatg ctattctggt agaagccaaa
     8041 accggatcgc agcgcgcgtt gatcgattat cgcaaaaatc ctccacagcc atacaatcat
     8101 caaaaagtac cgggtaacgt ctgggatttt ccgcgcgtgc gttatttgat ggatgaatat
     8161 gagaaccacc cgacgcaaaa accggaagcc ttactgaaac gcattattct cgcctcttcc
     8221 aacccaggcg atatcgttct cgacccgttt gccggtagct ttactaccgg tgccgtagcc
     8281 gtctccagcg ggcgaaaatt cattggtatt gagatcaaca gcgagtacat caaaatgggg
     8341 cttcgacggc tggatgtcgc gtcgcattac tctgcggaag aactggcgaa agtgaaaaaa
     8401 agaaagaccg gcaacctgtc aaaacgaagc cggttaagcg aagttgaccc cgatctcatt
     8461 gcaaagtaaa ggaagtgtaa gcctggtttt cagattattc atttcgtgta tatttcctgc
     8521 cagacttggt taaacatgca caggctcagg taatgattcg caagtattgg tggctcgtcg
     8581 ttttcgctgt cttcgttttt ctgtttgata ctttactgat gcagtggatt gaactgctga
     8641 caacagaaac agacaaatgc cgtaatatga actcagttaa tccactaaaa ctggttaact
     8701 gtgacgaact gaattttcag gacagaatgt gaatttacat gacacttaat tcattcgttt
     8761 gatgaattaa tttcgttatg ttttcatctg gcatgaacat tcttaatacg ttatcgacca
     8821 gcgcgggtgc ttgtttataa agatcataac ccgccatatt cattaaccag ttttgaacaa
     8881 ttccgctgaa ggcaccatta ataataatca tcacaacatc taaatcgagg ttatttgcta
     8941 cacaaccttg ttgctgacac gcctgcaata cttcgcggag agtctgcgga ttaaagccca
     9001 tcttttcgcg tatcactccc tcggccagca tctcatcatt aaattcacat ttgtgatata
     9061 agattttcag caacgcctgc tggcggggaa ttttggcaat atattgcaag ccgacaatca
     9121 atttttcacg caattgttga aacgggtcat gctctaatcc agccgtcaag tgttcctgga
     9181 ttaactcccg caatgaaggc tgttgcaacc acatctcatt aaacagttga gtcttgtttt
     9241 cgaagtgcca gtagatagcg ccacgcgtaa cgttagcggc gtcggcaatg tcgttgagcg
     9301 tcgtcttgct tacgccatgc tgcgcaaact gggcgatggc agtttcaatc agttcttgcc
     9361 gggtcttcag agcttcggct ttggttcttt ttgccatgat taattattca ggaaataaat
     9421 atattcgaca cagggtgaga aaatagcgaa ggttaatcta tcatctaatg tgtatttata
     9481 cgagagggta acattgagtt gctataaatc gtcaaataaa caatgtatat tatttaccta
     9541 aaatacattc gccacatgaa tatatatttc aatttacgag gttttaattc tgcctctttc
     9601 aacccgcgtc aaaataaaac agtagaatat taatcttttt ttgtgtttat gtgccttgag
     9661 acgccagtat tcataactat tccttacatc gacgaatgat aatttgtagg atagcaaact
     9721 gtatttttct ttctgcgagt taacgcgttg cctttctggg taaataacgc gcttttggtt
     9781 ttttgaggaa tagtaatgac gaaacatgcc aggtttttcc tcctgccctc ctttattctg
     9841 atctccgcgg ctttaatcgc cggttgtaac gataagggag aagagaaagc tcacgtcggt
     9901 gaaccgcagg ttaccgttca tattgtgaaa acggccccgt tagaagttaa gactgaatta
     9961 ccaggccgca ccaatgctta ccgtatagcc gaagttcgcc cacaggttag cgggatcgta
    10021 ctgaatcgta atttcactga aggcagcgat gtacaagcag gccagtccct gtaccagatc
    10081 gatcccgcga cctatcaggc aaattataac agcgcgaaag gcgaactggc gaaaagtgaa
    10141 gccgccgccg ccatcgcgca tttgacggta aaacgttacg ttccgctcgt gggtacgaaa
    10201 tacatcagcc agcaggagta cgaccaggcc attgctgatg ctcgccaggc cgatgccgcc
    10261 gtgattgccg caaaaggcac agtcgaaagc gctcgcatca atctcgctta taccaaagtg
    10321 actgcgccaa ttagcggacg tatcggcaaa tcgaccgtga ccgaaggcgc tcttgtcact
    10381 aatgggcaaa cgactgaact ggcgactgtc cagcagctcg atcctatcta cgttgatgtg
    10441 acccaatcca gcaacgattt tatgaggctg aagcaatccg tagagcaagg aaatttgcat
    10501 aaggaaaacg ccaccagcaa cgtagagttg gtcatggaaa acggtcaaac ctatcccctg
    10561 aaaggtacgc tgcaattctc tgatgtgacc gttgatgaaa gcaccggctc cataacccta
    10621 cgtgctgtct tccctaaccc gcaacatacg cttttgccgg gtatgtatgt gcgtgcacgg
    10681 attgatgaag gcgtccaacc tgacgccatt cttatcccgc aacaaggcgt tagccgcaca
    10741 ccgcgtggtg atgcaaccgt gctgattgtt aacgataaaa gtcaggtgga agcgcgccct
    10801 gtcgttgcca gtcaggcgat tggcgataaa tggttgatta gcgaaggtct gaaatctggc
    10861 gatcaagtca ttgtcagcgg cctgcaaaaa gcgcgtccgg gagagcaggt taaagccact
    10921 accgataccc ccgcagatac tgcatcgaag taaggtaatc tgacatggca aactttttta
    10981 ttcgacgacc gatatttgca tgggtgctgg ccattattct gatgatggcg ggcgcactgg
    11041 cgatcctaca attgcccgtc gctcagtatc caaccattgc accgcctgcg gtttctgttt
    11101 cggcaaacta tccaggtgct gatgcgcaga ccgtgcagga tacggtgacg caggttatcg
    11161 aacagaatat gaatggtatc gataacctga tgtatatgtc ctccaccaac gattccgccg
    11221 gtagcgtgac aattaccctt accttccagt ccgggaccga tcctgatatc gcgcaagtgc
    11281 aggtgcagaa caaactccag ctcgccacgc cgttgctgcc gcaggaggtt cagcagcagg
    11341 ggatcagtgt tgaaaagtcc agtagcagct atttgatggt ggcgggtttt gtctctgata
    11401 acccaggcac cacacaggac gatatctcgg actatgtggc ctctaacgtt aaagatacgc
    11461 ttagccgtct gaatggcgtc ggtgacgtac agcttttcgg cgcacagtat gcgatgcgta
    11521 tctggctgga cgccgatctg ctaaacaaat ataaactgac accggttgat gtgattaacc
    11581 agttgaaggt acagaacgat cagatcgctg ccggacagtt gggcggaacg ccagcgttac
    11641 cagggcaaca gttgaatgcc tcgattattg ctcaaacgcg gttaaaaaat ccggaagaat
    11701 tcggcaaagt gaccctgcgc gtaaacagtg acggctcggt ggtacgcctg aaagatgtcg
    11761 cacgggttga acttggcggt gaaaactata acgttatcgc tcgtatcaac ggaaaaccgg
    11821 cggcgggcct ggggattaag ctggcaaccg gcgcgaatgc tctcgatacc gcgaaagcca
    11881 ttaaggcaaa actggcggaa ttacagccat tcttcccgca gggaatgaag gttctctacc
    11941 cttatgacac cacgccattc gtccagcttt ctattcacga agtggtaaaa acgctgttcg
    12001 aagccattat gctggtgttc ctggtgatgt atctgttctt gcagaatatg cgagcaacgc
    12061 tgatccccac cattgcggta cccgtggtgt tgttagggac gtttgccatc ctcgccgctt
    12121 ttggttactc catcaacaca ctaacgatgt tcgggatggt gcttgccatc gggctgctcg
    12181 tcgatgatgc gatagtggtg gtggagaacg tcgagcgcgt gatgatggag gataagctcc
    12241 cgccaaaaga agcgacggaa aaatcgatgt cgcaaattca gggcgcactg gtgggtatcg
    12301 cgatggtgct gtcagcggta tttattccga tggcattctt cggcggttct actggggcaa
    12361 tttatcgcca gttctctatc accatcgttt cggcaatggc actttctgtt ctggtggcat
    12421 tgattcttac ccctgcgtta tgtgcaacgc tgcttaaacc cgtctctgct gagcatcacg
    12481 aaaataaggg cggtttcttc ggctggttta ataccacctt cgatcatagc gttaaccact
    12541 acaccaacag cgtcggcaaa atcctcggtt ccacaggacg atatttactg atctatgcgc
    12601 tgattgttgc aggaatggtg gtgttgtttt tacgtcttcc gtcttccttc ttacctgaag
    12661 aggatcaggg tgtctttctg accatgattc agttacccgc tggcgcgacg caagagcgga
    12721 cgcaaaaagt gttggatcaa gtgacggatt actatctgaa gaacgagaaa gcgaacgttg
    12781 aaagtgtctt tacggttaac ggctttagct tcagcggcca ggcacaaaac gccggtatgg
    12841 ccttcgtcag tctgaaaccg tgggaagagc gtaatggtga tgaaaacagt gcggaagcgg
    12901 taatccatcg tgccaaaatg gaattgggca agatccgcga cggttttgtc attccattca
    12961 atatgccagc cattgttgaa ctgggcacgg caacgggttt cgactttgag ttaattgatc
    13021 aggctgggct gggtcacgat gccctaaccc aggcccgtaa ccagttgctt ggtatggcgg
    13081 cgcaacatcc tgccagctta gtcagcgtgc gtcctaatgg cctggaagac accgcgcagt
    13141 ttaaactgga agttgaccag gaaaaggcgc aggcattaag tgtttcactt tctgacatca
    13201 atcagaccat ttcaacggcg ttgggtggga cttacgttaa cgacttcatc gaccgtggtc
    13261 gcgtgaaaaa ggtgtatgtt caggcggatg ccaaattccg tatgctgcca gaagatgtcg
    13321 ataaacttta tgtccgcagc gccaacggcg aaatggtgcc attctcggcc tttaccactt
    13381 cacattgggt gtatggctct ccgcgactgg aacgctacaa cggtctgccg tcaatggaga
    13441 ttcaggggga agccgcgcca ggaaccagtt ccggcgatgc catggcgttg atggaaaacc
    13501 ttgcgtcaaa attacctgcg ggcattggtt atgactggac gggtatgtcg tatcaggaac
    13561 gcttatcggg aaaccaggct cccgctctag tagcaatttc ctttgtggtt gttttcctgt
    13621 gccttgctgc actctatgaa agctggtcaa ttcctgtctc ggttatgttg gtcgtgccgt
    13681 tagggattgt cggcgtgctg ctggcggcga cactctttaa tcaaaaaaat gacgtctact
    13741 ttatggtggg cttgctaacg acaattggct tgtcggccaa aaacgctatt ttgattgttg
    13801 agttcgctaa agatctcatg gagaaagagg gtaaaggtgt tgttgaagcg acactaatgg
    13861 cagtacgtat gcgtctgcgt cctatcctga tgacctctct cgcctttatt ctcggcgtat
    13921 taccgctagc tatcagtaac ggtgcaggca gtggcgcgca gaacgcagtg ggtatcgggg
    13981 taatgggcgg aatggtctct gcaacgttgc tggcaatctt cttcctaccg gtgttctttg
    14041 tggtgatccg ccgttgcttt aaaggataaa tcagaaacat aaaggcactt tcgggtgcct
    14101 ttattatttt cagtgaaacc cataaaaatt aataagatat tcttccactc actctttaaa
    14161 aactttctat agttcccgct cccttcacta tttttacaat tcacataatt tgtcattatt
    14221 cattccgcaa tatttataac ctccggtgat aaaatggcat tcagctcgtt aataagagag
    14281 ttaacttatt aagcgttagc gtttattact gaggtaacac catgaaaaga ttaattcctg
    14341 tggcattgct caccgcattg ctggcgggct gtgctcacga ttcgccctgc gttccggttt
    14401 atgacgatca gggttgtctg gttcacacca atacctgtat gaaaggcacg actcaggata
    14461 actgggaaac ggctggggct attgccggcg gggcagcggc tgttgctggc ctgacgatgg
    14521 ggatcatcgc tatgtcgaaa taactgaaca gggcgcggag tttcgcgctc tgtttaacga
    14581 tacagtatct ttacataaga taaaatattg ctatattctg ctggctaatt ctctgctgat
    14641 ttagcatgtt atatcgcgtt atttcgctgc tttcgaaata acgcatcaat ttaactttca
    14701 cacttcccac cagaaatata ctcacgctaa caatcattat tctgcttagt aaaacacgcg
    14761 taaatatttt gcgccaaaat gtggcgcatg tttcattttc gcaccattgc ggggcgctgt
    14821 ttttattatt tcctgtctac actcagctta ttgcgacgcg aaatcgtgcg caaaagctgg
    14881 cactactttt gcttataaga ggatggccac agacaggtaa aagacgtttc ccaaacgtcc
    14941 tataacgata attttcgcct cacaggaagc attatgaaaa agatgatgat agccacactg
    15001 gctgccgcca gcgtgctgct tgccgttgca aatcaggcgc atgctggcgc gacgcttgat
    15061 gccgttcaga aaaaaggttt tgtgcaatgc gggatcagtg atggattact tgggttctct
    15121 tatgccgatg cagacggtaa gttttcaggt attgatgttg atgttgatgt tgatgttgat
    15181 gttgatgttt gtcgtagtgt tgccgctgct gtatttggtg acgacacgaa agtgaaatat
    15241 accccactca cagcaaaaga acgcttcacg gctttacagt caggggaagt ggatttgctc
    15301 tcccgtaata cgacctggac ttcatctcgc gacgccggga tgggaatggc atttactggc
    15361 gtcacttatt acgacggcat tggcttcctg acgcacgata aagcggggct aaaaagcgcg
    15421 aaagaactgg atggcgctac cgtctgtatt caggcgggta ctgataccga actcaacgtt
    15481 gccgactatt tcaaggcaaa caatatgaag tacacaccgg tgactttcga tcgctctgac
    15541 gaatcagcga aggcactgga atctggtcgc tgcgatacgc tggcctcgga tcagtcgcaa
    15601 ctgtatgccc tgcgcatcaa attaagcaac ccagctgaat ggattgtctt accggaggtt
    15661 atctctaaag aaccgcttgg gccggtagtt cgtcgtggcg atgatgaatg gttctcgatt
    15721 gtacgctgga cgcttttcgc catgctgaat gctgaagaga tgggcatcaa ttcccagaac
    15781 gtcgatgaaa aagcggctaa tccagcaacg cctgatatgg cacatctgct gggtaaagaa
    15841 ggcgattacg gcaaggatct gaagttggat aataaatggg cctataacat catcaaacag
    15901 gtgggtaact actcggaaat tttcgagcgt aacgtaggtt cagaaagccc gctgaaaatt
    15961 aaacgtgggc aaaataatct ctggaataac ggcggtattc agtacgcgcc gcccgtgcgt
    16021 taagtcggtg tgaggtaacg agcgtcgcta cggcggcgct ctcttcagcg ttatgctttc
    16081 cgaggtttct atgtctcatc gccgctcaac cgttaaaggc tcactctcct ttgccaaccc
    16141 tacggttcgt gcctggttat tccagatcct tgccgttgtt gctgttgtcg gaattgttgg
    16201 ttggttattt cacaacactg taacgaatct cagtaatcgt ggcattactt caggttttgc
    16261 ctttctggat cgcggcgctg gcttcggtat tgtccagcat ttgatcgatt accagcaggg
    16321 cgacacctac ggacgcgttt ttattgtcgg cttactcaat acgctactgg tttctgcatt
    16381 gtgtattgtg ttcgcttctg ttttaggctt ctttatcggt ctgacgagac tttcggataa
    16441 ctgactgcta cgaaagctat ccacaattta tattgagatc ttccgtaata ttcccccgct
    16501 gctgcaaatc ttcttctggt actttgccgt gttgcgcaat ttgcccggac cacgcgccag
    16561 gctgtgagcg cgtttgatct ggcctttttg agcaatcgtg ggctttatat tccgtcaccg
    16621 cagctgggag acggatttat tgcgtttatc ctagctgttg ttatggctat agtcctttct
    16681 gttgggctat tccgctttaa taaaacatac cagataaaga ccggacaact gcgccgcacc
    16741 tggccgttcg ccgcagtgtt gatcatcggt ttgcctttac tggcgcaatg gctttttggc
    16801 gcagcactac actgggatgt cccagcccta cgaggcttta acttccgcgg cggaatggtt
    16861 ttaattcctg aactggcagc cttaacgctg gcactttcgg tttatacctc tgcatttatc
    16921 gccgagatta tccgcgctgg gatccaggca gtgccttatg gtcaacatga agcggctcgg
    16981 tcactggggt tacgctacgc caggtcatta ttccccaggc actgcgagtg attattccgc
    17041 cgttaaccag ccagtatctc aacatcgtca aaaactcctc tcttgccgcc gctattggct
    17101 atcccgatat ggtttcgctg tttgctggca ccgtgctgaa ccagacgggg caagccatcg
    17161 agacgatagc catgaccatg tcggtctatc tgattatcag cctgactatc tcgctgctga
    17221 tgaatatcta taaccgccgc atcgcgatcg ttgaacgcta aggagtcatg atgacaaaag
    17281 tattgctgtc tcagccctcg cgcccagcga gccataactc aagccgcgcg atggtgtggg
    17341 tgcgaaaaaa tctgttctcc agctggagca atagcctgct gactattggc tgcatatggt
    17401 tgatgtggga actgatccca ccgttgctga actgggcatt tttgcaggct aactgggttg
    17461 gctcaacgcg tgccgactgc acaaaagccg gtgcctgttg ggtcttcatc cacgaacgat
    17521 ttggtcagtt tatgtatgga ctttacccac acgaccaacg ctggcgaatt aacctagtat
    17581 tactgattgg ccttgtgtca atcgcaccaa tgttctggaa aatactcccg catcgcggtc
    17641 gctatattgc ggtctgggcg gtgatttacc cactgattgt ctggtggctg atgtatggcg
    17701 gtttttttgg tcttgagcgg gttgaaaccc ggcaatgggg cgggctgacg ctaactttaa
    17761 ttattgcatc cgttgggatt gcgggggcgc taccgtgggg gatcttactg gcgttaggtc
    17821 gccgctccca tatgccgatt gtgcgtatct tatcggtcat ttttatcgag ttctggcgcg
    17881 gcgtaccgct gattaccgta ttgtttatgt cttcggtcat gctgccgttg tttatggcag
    17941 aaggcaccag tatcgacaaa ctgatccgcg cgctggttgg cgtgatcctg tttcagtcag
    18001 catatgttgc ggaagtcgtg cgaggcggat tacaggccct acctaaaggg caatatgaag
    18061 cggcagagtc gctggcgttg gggtactgga aaactcaggg gctggttatt ctgccacagg
    18121 cgttgaagct ggtaattcct gggctggtaa ataccatcat cgcactcttc aaagatacca
    18181 gcctggtgat catcatcggc ttgttcgatc ttttcagtag cgttcagcag gcaaccgttg
    18241 atcccgcctg gttgggtatg tcgacggaag ggtatgtttt cgccgcgctg atctactgga
    18301 tcttttgttt cagcatgtca cgttatagcc aacatctgga aaaacgtttt aacaccgggc
    18361 gtacaccgca ttgaggacac tatgagccaa attttactgc aacctgctaa cgcgatgatt
    18421 acgctggaaa acgtccataa atggtatgga caattccatg ttttgaaaaa tataaattta
    18481 accgtgcaac cgggagaacg gatcgttctg tgtggccctt ctggttccgg taaatcgaca
    18541 accattcgtt gtattaatca tctggaagaa catcaacagg gacggattgt cgtagatggc
    18601 atcgaactta atgaagatat ccgcaatatt gagcgcgtca ggcaagaagt gggaatggta
    18661 tttcagcatt tcaatctctt ccctcatctg accgttttac agaactgtac cctggcaccg
    18721 atttgggtac gcaagatgcc taagaaagag gctgaagctc tggcgatgca ttaccttgag
    18781 cgggtgagaa ttgccgaaca tgcgcataag tttcccggac agatttcagg tggtcagcag
    18841 caacgcgttg ccattgcgcg ttcgctgtgt atgaagccga aaattatgtt gtttgatgaa
    18901 ccaacgtcgg cctcgatccg gagatggtga aagaggtgct ggatacgatg attgggctgg
    18961 cgcagtcggg tatgacaatg ttgtgtgtaa cacatgagat ggggtttgca cgtaccgtcg
    19021 ctgaccgggt aatttttatg gatcgtgggg agatagtgga acaagctgca cctgatgaat
    19081 tttttacgca tcctaaatca gagcgtacga gggcattttt atcgcaggta atccattaat
    19141 tgaatgttag ttcgaaaagc aaaaaggcca tcctttcgga tggccttttt gcttgatttg
    19201 atgtctggca gtttatggcg ggcgtcctgc ccgccaccct ccgggccgtt gcttcgcaac
    19261 gttcaaatcc gctcccggcg gatttgtcct actcgagaga gtgttcaccg acaaacaaca
    19321 gataaaacaa aaggctcagt cggaagactg ggccttttgt tttatttgat gtctggcagt
    19381 tccctactct cgcatgggaa gaccccacac taccatcggc gctacggcgt ttcacttctg
    19441 agttcggcat ggggtcaggt gggaccaccg cgctactgcc gccagacaaa ttcttttcta
    19501 atttgccgaa ctttaaccta aaaagtggtg ctgataccca gagtcgaact ggggacctca
    19561 cccttaccaa gggtgcgctc taccaactga gccatatcag cacgct
//
LOCUS       DAJTMC010000071        19529 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.071, whole genome shotgun sequence.
ACCESSION   DAJTMC010000071 DAJTMC010000000
VERSION     DAJTMC010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19529
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.071"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(291..728)
                     /locus_tag="OO130_002204"
     CDS             complement(291..728)
                     /locus_tag="OO130_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HCR5365606.1"
                     /translation="MNRVLCVVIIALLVACGALSLGLNHYRDNAITYKEQRDKATSTI
                     ADMQKRQRDVAELDARYTKELADANATIESLRADVSAGRKRLQVAATCAKSTTGASGM
                     GNGESPRLTADAELNYYRLRSGIDKITAQVNYLQEYIRTQCLK"
     gene            complement(1027..1560)
                     /locus_tag="OO130_002205"
     CDS             complement(1027..1560)
                     /locus_tag="OO130_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HCR5365607.1"
                     /translation="MNAKIRYGLSAAVLALIAAGAPAPDILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGAILVDGKPVVPGMKLSKEKCDRVNAIERDKALAWVEKNIRVPLSEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRINAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWGIDR"
     gene            complement(1624..1974)
                     /locus_tag="OO130_002206"
     CDS             complement(1624..1974)
                     /locus_tag="OO130_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HCR5365608.1"
                     /translation="MTQNYKLIVKGIRNFENKVTVTLALRDKKRFDGEIFDLDISLDR
                     VEGAALEFYEAAARRSIRQVFLDVAAGLCEGDEQSPEKRPVILEAQNVWITYKGKLPG
                     RITGSLKTPPESQP"
     gene            complement(1979..2194)
                     /locus_tag="OO130_002207"
     CDS             complement(1979..2194)
                     /locus_tag="OO130_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HCR5365609.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
     gene            complement(2344..4197)
                     /locus_tag="OO130_002208"
     CDS             complement(2344..4197)
                     /locus_tag="OO130_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HCR5365610.1"
                     /translation="MAFKHYDVVRAASPSDLAKQLTQKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVVVSGATEPEWYYVIVLAGQSNAMAYGEGLPLPDSYDAPHPRIKQLARRN
                     TVTPGGEVCVFNDIIPADHCLHDVQDMSTINHPRADLSKGQYGCVGQGLHIAKKLLPY
                     IPNNAGILLVPCCRGGSAFTQGTEGTFSESTGASQDSARWGVGKPLYQDLLFRTKAAL
                     QKNPKNVLLAICWMQGEFDMTNASYAQQPAAFLAMVQQFRADLAGLAAQCHGGSPASV
                     PWICGDTTYAWKQEHGTQYEVVYGAYKGKESQQIYFVPFMTDGSGVNTPTNNPSEDPD
                     IAGSGYYGSASRTNKNWVSSNRPTHFSSWARRGIIPDRMATAILNVAGRTLAFISGKA
                     PEIKPSPGGDTPSGPSEDASVRTISLLPTAGDAAAQGWSIKNGGIQLSDGVFKITKQS
                     NKAWSLTRPVDDAVSLLTRGGRLSCKFRLSGALTNNQFGLGIYLYTDVALPDVVAMTG
                     TGNPFLMSFFTQTTDGKLNLMHHKKAGNTKLGEFGNYSNDWQTLELVFTAGSATVTPK
                     LNGVAGPAFQVIKDSLTVGLNALTLTDITKNATYGVEIESLVLEINAPASS"
     gene            complement(5173..5249)
                     /locus_tag="OO130_002209"
     tRNA            complement(5173..5249)
                     /locus_tag="OO130_002209"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5213..5215),aa:Arg,seq:tct)
     gene            complement(5263..5339)
                     /locus_tag="OO130_002210"
     tRNA            complement(5263..5339)
                     /locus_tag="OO130_002210"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5303..5305),aa:Arg,seq:tcg)
     gene            complement(5347..5422)
                     /locus_tag="OO130_002211"
     tRNA            complement(5347..5422)
                     /locus_tag="OO130_002211"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5387..5389),aa:Ile,seq:cat)
     gene            complement(5472..6522)
                     /locus_tag="OO130_002212"
                     /pseudo
     CDS             complement(5472..6522)
                     /locus_tag="OO130_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001575625.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
     gene            complement(6630..6870)
                     /locus_tag="OO130_002213"
                     /pseudo
     CDS             complement(6630..6870)
                     /locus_tag="OO130_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309113.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7097..7918)
                     /locus_tag="OO130_002214"
     CDS             complement(7097..7918)
                     /locus_tag="OO130_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCR5365611.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRERATELLTEYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESVVFTNKVQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGC
                     ERLPSTEAFNAICKVTSAITLDTWKKSVKRFYDTLVVRFDIEEAWAERQLKRVTR"
     gene            complement(7915..8295)
                     /locus_tag="OO130_002215"
     CDS             complement(7915..8295)
                     /locus_tag="OO130_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCR5365612.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRAVALIVR
                     QQRLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLMDDEQFDEINIVR
                     AQPVSGGRLGVKIYPIMLEGQVKK"
     gene            complement(8296..9279)
                     /locus_tag="OO130_002216"
     CDS             complement(8296..9279)
                     /locus_tag="OO130_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCR5365613.1"
                     /translation="MPVFHNTRVLVEPEPKSMRNLPSGVVPAVRQPLAEDKSLLPFFS
                     DERVIRAAGGAGALSDWLLRHVKSCQWPHGDYHHSETVIHRYGTGAMVLCWHCDNQLR
                     DQTSESLGQLAHQNLFAWMIDVIRHAMNGSQERELSLAELSWWAVRNQVADALPEAVL
                     RRSLGLRAEKIRSMYRESDIVPGEQTATSILKQRTKNLAPLPHAHQQNPPQEKTVVSI
                     AVDPESPAQYLQRQKSQREEMPVYTRWVKTQKCMTCGNQADDPHHIIGYGLGGMGTKA
                     DDLFVIPLCRKCHSELHAGVKDFEEKHGSQLLLLIRFLMHARNSGVLKWKA"
     gene            complement(9353..9625)
                     /locus_tag="OO130_002217"
     CDS             complement(9353..9625)
                     /locus_tag="OO130_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5365614.1"
                     /translation="MAHKTKFHNSDTDLAIRHGRRSHAFKTDWYQHPPCTEEQAEWLI
                     HNYRRRGYEIKKALSLDYRHWIISVRLPYSERPPRPSRTFQQRIWR"
     gene            complement(9793..9948)
                     /gene="hokD"
                     /locus_tag="OO130_002218"
     CDS             complement(9793..9948)
                     /gene="hokD"
                     /locus_tag="OO130_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HCR5365615.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            complement(10186..10851)
                     /locus_tag="OO130_002219"
     CDS             complement(10186..10851)
                     /locus_tag="OO130_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HCR5365616.1"
                     /translation="MTIADFKRPKLELPNGANKLLLHSCCAPCSGEVMEALQASGIDY
                     TIFFYNPNIHPQKEYLIRKDENIRFAEQHGVPFIDADYDTDNWFERAKGMEWEPERGI
                     RCTMCFDMRFERTALYAAENGFSVISSSLGISRWKNMQQVNECGRRAVAHYPGMVYWD
                     YNWRKQGGSSRMIEISKREKFYQQEYCGCVYSLRDTNLHRKSQGRPLIKIGQLHYGKE
                     EKE"
     gene            complement(11026..11451)
                     /locus_tag="OO130_002220"
     CDS             complement(11026..11451)
                     /locus_tag="OO130_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HCR5365617.1"
                     /translation="MAKVFTQEEREKIKGQVVELVRLSGRETLRQLEAKTGATRYLMS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKVKKPAVVDPDLIWSLPDG
                     EIRRYDRRLNIICRECRKSEAMQRVLAFYQGNVRYFRRY"
     gene            complement(11467..12237)
                     /locus_tag="OO130_002221"
     CDS             complement(11467..12237)
                     /locus_tag="OO130_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HCR5365618.1"
                     /translation="METVSDALKALKKASSHVVAARLGISREEAVNELWELKRKGVVD
                     KTGHTWFLAGEGESRVTEERPVKSEAQDMLTGEVEQKVTADMMIEFIGQDGAKTCEEL
                     AGKFGVSTRKVASTLAVVTATGRLARVNQNGKFRYCMPGDNLPAEPKAALVTESDGKA
                     FPQPAGAALPVREAATQEEIKTETVADIVQPLPSFTETQADELIFPSLRRANLALRRA
                     KSDVQKWERVCAALRELNKHRDIVRQITDSSRRVVSEK"
     gene            complement(12259..13001)
                     /locus_tag="OO130_002222"
                     /pseudo
     CDS             complement(12259..13001)
                     /locus_tag="OO130_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309101.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            complement(13008..13970)
                     /locus_tag="OO130_002223"
     CDS             complement(13008..13970)
                     /locus_tag="OO130_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5365619.1"
                     /translation="MSSKLHGLVWEGCAFTGMILSRVAVMARLADYSNDEGVSWPAIE
                     TIRRQIGARSESTVKSAIAELAKEGWLTKEERKVGGRNVSNIYRLNVEKLEAAAAAAR
                     ESYKPKRKISPAKNDPLTVDPSNIDPSTVDPSNFDGSTVDKKLPIRGAMIDPDPSVLK
                     PDPSDKRSSCPDASQPDPQTAEQDFLTRHPDAVVFSAKKRQWGSQEDLVCAQWIWGRI
                     VSLYEQAASYDGEITRPKEPNWTAWANDVRTMRMLDGRTHRQICEMFGRLQRDSFWVK
                     NIMSPAKLREKWDELVIRLGRSPAQRCVNHISEPDTEIPPGFRG"
     gene            complement(13993..14418)
                     /locus_tag="OO130_002224"
     CDS             complement(13993..14418)
                     /locus_tag="OO130_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCR5365620.1"
                     /translation="MKIKHEHIRMAMNAWAYPDGEKVPAAEIARTYFELGMTFPELYD
                     DSHPEALARNTQKIFRWLDKDPPDAVEKMQALLPAIEKAMPPLLVARMRSHSSEYYRE
                     IVERRDRLVKDVDDFVASAVVLYDQMNRGGPAGNAVVMH"
     gene            complement(14402..14725)
                     /locus_tag="OO130_002225"
     CDS             complement(14402..14725)
                     /locus_tag="OO130_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR5365621.1"
                     /translation="MKKDNYSFKRACAVVGGQSAMARLLGVSPPSVNQWIKGVRQLPA
                     ERCPAIERATKGGVLCEELRPDVDWTYLRRSSCYSQNMSMKQPNDENDHTRSIKRQMI
                     HENQT"
     gene            14850..15326
                     /locus_tag="OO130_002226"
     CDS             14850..15326
                     /locus_tag="OO130_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5365622.1"
                     /translation="MLSGKDLGRAIEQAINKKIASGAVKSKAEIARHFKVQPPSIHDW
                     IKKGSISKDKLPELWRFFSDVVGPEHWGLNEYPIPTPSTSDTKSELLDINSLYQAASD
                     EKKAIVAFILSGNATEPSWVDYDVRAYIAAMEMKVANYLKNQESKRKSQNITKTGT"
     gene            15645..15797
                     /locus_tag="OO130_002227"
     CDS             15645..15797
                     /locus_tag="OO130_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HCR5365623.1"
                     /translation="MDTIELGNSESLVCGVFPNQDGTFTAMTYTRSKTFKTEAGARRW
                     LTRNTD"
     gene            15809..16129
                     /locus_tag="OO130_002228"
     CDS             15809..16129
                     /locus_tag="OO130_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5365624.1"
                     /translation="MEFKDLPVPFQEMASNVVRSQLATLDLSTVEKETIDNISGNVRR
                     AFIGLYEEKLLSDNQDLHEKYFLDLMDIIDKGFGLLMKKKGIRIEPLENYFATKSINS
                     FDSK"
     gene            complement(16107..16382)
                     /locus_tag="OO130_002229"
     CDS             complement(16107..16382)
                     /locus_tag="OO130_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5365625.1"
                     /translation="MSTENDEIINSLIRQINNFDKALQHAAARSDITLLAISFLASVM
                     DKNEVVRQSLVDYIDSLQPGTFNHESFNHEKEHVKSVINSLILNQKN"
     gene            16944..17132
                     /locus_tag="OO130_002230"
     CDS             16944..17132
                     /locus_tag="OO130_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000854559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HCR5365626.1"
                     /translation="METLLPNINTSEGCFEIGVTISNPVFTEDAINKRKHERELLNKI
                     CILSMLARLRPIQKGCWQ"
     gene            17129..17320
                     /locus_tag="OO130_002231"
     CDS             17129..17320
                     /locus_tag="OO130_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HCR5365627.1"
                     /translation="MNTAFALVLTVFLVSGEPVDIAVSVHRTMQECVTAATEQKIPGN
                     CYPVDKVIHQDNNEIPAGL"
     gene            17413..>19529
                     /locus_tag="OO130_002232"
     CDS             17413..>19529
                     /locus_tag="OO130_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HCR5365628.1"
                     /translation="MSKVFICAAIPDEQAIKEEGAVAVATAIEAGDERRARAKFHWQF
                     LEHYPAAQDCAYKFLVCEDKPGIPRPALDSWDAEYMQENRWDEESASFVPVETESDPM
                     NVTFDKLAPEVQNAVMVKFDTCENITVDMVISAQELLQEDMATFDGHIVEALMKMPEV
                     NAMYPELKLHAIGWVKHKCKPGAKWPEIQAEMRIWKKRREGERKETGKYTSVVDLARA
                     RTNQQHSENSTGKISPVIAAIHREYKQTWKTLDDELAYALWPGDVDAGNIDGSIHRWA
                     KNEVIDNDREDWKRISASMRKQPDALRYDRQTIFGLVRERPIDIHKDPVALNKYITEY
                     LTTKGVFEDEGRNQSATDTLSSPVPETDAVETAIPGNEKTECKVEVEPSVEREGPFYF
                     LFTDKDGEKYGRANKLSGLEKALALGATEITKEEYFARKNGTYSGSQQNTGASDTTAQ
                     PEPVKVTADEVNKIMQAANISQPDADKLLAASRGEFVAGISDPNDPKWVKGIETRDSV
                     NQNQQESEQNDQKAEQNSPNTQQNEPETKQPEPVAQQEPEKVCTACGQSGGGNCPDCG
                     AVMGDATYQETFDEENQVEVQENDPKEMEGAEHPHKENAGSAQDHASDSETGETADPL
                     IAMNGHHVITSTSRTCDHLMIDLETMGKNPDAPIISIGAIFFDPQTGDMGPEFSKTID
                     LETAGGVIDRDVIKRWLKQSREA"
BASE COUNT         4855 a         5219 c         4541 g         4914 t
ORIGIN      
        1 gtgattacct tttgtctgga aaactgacca ttccacgaag atcagatagg tctatagaat
       61 cttggtttgt catagcgttc gccttacttt gagatgaacc tttgctgcat aggagatcag
      121 cccgtcaagg ctcaccagca ctaactgact actcaaaggc tcattccaaa gggtttggtt
      181 cgacgtggtt gagtgcactg cggtgcgcgg tgaaatacct gtacaaaaat gccccgcatc
      241 tgcgaggcat tttcctgaaa gtcacgtatt aaatttcagt gaaattaaaa ttattttaag
      301 cactgcgtcc tgatgtactc ctgcaggtag ttgacctgcg cggttatctt gtcgattcca
      361 cttcggagac ggtaataatt gagttcagca tctgctgtaa gtcttgggct ttctccattg
      421 cccatgccgc tggctccggt cgttgacttt gcacaggtag cggcgacttg caggcgctta
      481 cgcccagcag aaacatcagc acggaggctt tcgatagtcg cgttagcatc agcaagctcc
      541 tttgtatatc tggcatcgag ttctgctacg tcacgttgac gcttctgcat atcagcgatt
      601 gtggatgtgg ctttatcgcg ctgctctttg taggttatgg cgttatcacg gtaatgattc
      661 agccccagac taagcgcacc acaggccacc agcagggcaa tgatgaccac gcacagtacg
      721 cggttcattt caccaccagc gtatctgacc gatgaaataa ccggaggcca taatcacaaa
      781 caccagccag ataagaatga acttccaggt ggataatttt tcagccatca ctcgaatctc
      841 ccgaatcagt ttgctaaaat caaacacact ttctcctttg acttttccgg agtcaggaaa
      901 cacaaaaccc cgcttgcagc caacaaacgg ggtttttact tttattctct tagtttttgt
      961 cagttcgcag gatttcgtgt tatccgtccg tgtgagcaaa ccgcattttt cagcaaaata
     1021 ttctgcttat ctgtcaattc cccagcacgc cagcgcactc tcctggtcgc gacgggatac
     1081 ctgaccgtaa cagttgtttg agcgaatacg gcagtctctg ccaccgtcct taatccacca
     1141 gcgaatcgcc tcacacgctc ccctgcgatc acctgcatta attcgtttat aaaacgtcga
     1201 cggaaaacac ttaccggggc caatgttgta cggacagaat gacgcgatcc ccgctttctg
     1261 gggttcactc aatggcactc tgatgttttt ctccacccat gccagcgcct tatcacgctc
     1321 aatggcgtta acccggtcgc atttttcctt cgacaacttc atgcccggaa cgacaggttt
     1381 gccatccacc aggatggcac cgcggcaaat ggtccagata cccgcgccat cacggtatgc
     1441 cgtggtgtgg ttaccttcct tttcatccag aaactggtcg agaatgtcag gcgcaggcgc
     1501 accagcggca atcagcgcca gaacggcagc cgacaggccg tatctgattt ttgcgttcat
     1561 ggatatttat caggatttat cggtttctgc ccacggacag gtttatctgt tccggtcagt
     1621 gacttaaggt tgtgattccg gaggagtctt cagagaacca gtaattcttc ccggtagctt
     1681 tcctttgtag gttatccata cattctgcgc ctctaaaatt acggggcgct tttccggcga
     1741 ctgctcatcc ccttcacata acccggcagc aacatccagg aagacctgtc tgatgctcct
     1801 tctggctgct gcctcataaa actccagcgc ggcaccttca acacggtcca gcgagatgtc
     1861 caggtcaaaa atttcaccgt caaagcgttt tttgtcccgt aacgctaaag ttaccgtaac
     1921 tttattctca aaattgcgga tccctttcac aatcagttta tagttttgag tcattgaatt
     1981 actctccccg tgcagcctta cgacggtcct ctctgatttt gaaatacagg ttagtcagat
     2041 atgtcagcag cccaaacagc agactcccca gcacgcctat tgccgcccac tgagacgggg
     2101 aaaccctgtc cagcaactgc aggaaccagt agcccgttcc caccgctgac gtggtgtatg
     2161 acacacctgt tgtgattttt tccatctggt acataccccg tctcccgtta tccggaagct
     2221 gacaacaata aaaaagccac cagttaagta ctgatggctc tgataactca tgcaggcgtc
     2281 tcagacgacc cactgacact accggtgagt ttaacgatac cttccatttg actggctcac
     2341 tttttatgat gatgccggtg catttatctc cagcaccaga ctttctatct caacgccata
     2401 cgttgcattt ttggtaatat ccgtcagcgt cagtgcattt agtcccactg tcagactgtc
     2461 ttttatgacc tggaatgccg ggccagccac tccattcagt ttcggagtaa ccgtggcact
     2521 gccggcggtg aacaccagct ccagcgtctg ccagtcgtta ctgtaattcc cgaactcgcc
     2581 caactttgtg tttccggctt tcttgtgatg catcagattc agtttgccgt ctgtggtctg
     2641 ggtgaagaac gacatcagga acgggttacc agtcccggtc atcgccacga cgtcaggtaa
     2701 cgctacatcg gtatacagat aaattcccag accgaactgg ttgttggtca gtgcgcctga
     2761 cagtcgaaac ttacagctca gtctgccacc ccgtgtcagc agggagactg cgtcatccac
     2821 cgggcgcgtc agggaccagg ctttattgct ctgcttggtg atcttaaata caccatctga
     2881 caactgaatt ccgccattct taatgctcca gccctgcgca gcagcatctc cggctgtcgg
     2941 caacagggag attgtgcgta cggatgcatc ttcagacggc cccgatggcg tgtcgccgcc
     3001 gggcgagggt ttgatttccg gtgccttacc actaatgaag gctaaggtgc gaccggctac
     3061 gttcagaata gcagttgcca tacgatcggg aataatgcca cgacgcgccc atgagctgaa
     3121 atgcgtcggg cgatttgatg atacccagtt tttgttcgtt cgggatgccg aaccgtaata
     3181 accagacccg gcaatatcag gatcttctga cgggttgttt gtcggtgtat taactccgct
     3241 accatcggtc ataaagggaa caaaataaat ctgctgggat tctttacctt tatatgcacc
     3301 atataccact tcatattgcg taccgtgttc ttgtttccac gcgtatgtcg tgtcgccaca
     3361 aatccagggg actgatgccg gacttccacc gtgacactgc gctgccagcc cggcaaggtc
     3421 agcacggaac tgctgtacca ttgcaagaaa tgctgctggc tgctgggcgt aactggcatt
     3481 cgtcatatcg aattccccct gcatccagca tatcgccagc aaaacgtttt tcgggttttt
     3541 ctgcaatgct gccttcgtgc ggaaaagcag atcctgatat aacggcttac ccactcccca
     3601 gcgagccgaa tcctgactgg ctcccgtgga ctcgctgaat gtcccctccg tgccctgggt
     3661 gaatgccgaa ccaccacgac agcatggtac cagcaggatc cccgcattat tagggatata
     3721 cggaagcagt tttttggcaa tatgtaagcc ctgtccgaca cagccgtact gccctttgct
     3781 caggtcagcc cggggatggt taatcgtact catatcctga acatcatgca gacaatggtc
     3841 agcaggaatg atgtcgttaa atacgcatac ttcaccaccg ggagtcactg tgttacgacg
     3901 ggccagttgc ttaatgcgcg gatggggcgc atcgtatgaa tccggaagcg gaagcccttc
     3961 accgtaagcc atggcattgg attgcccggc cagtacgatg acgtagtacc actccggctc
     4021 agttgcacca ctgacgacca catcaccttc tgctgcaatc gcctgcatca gggtataagg
     4081 ggttatggcc accggactac caaacggctg ccagccctcc ttcagttttt gtgtcagttg
     4141 tttcgcaagg tctgacggcg atgccgccct gaccacgtca tagtgtttaa atgccatgaa
     4201 tcctcccggg cgggataatg ttgtgagtca gataaggagc aggctgaagt ccggaagtta
     4261 caggacaatg gcagaaggga gactacagcc cgcaattcga aaaagaccgc gcagttgcgc
     4321 agagtgatta ctatggggta ttattcgcca gctgaaatat tacttcacgt tttattgttt
     4381 attccttgcc gcccgcgtct cccagcgcgg gctttttttg tccataagaa agcccctccg
     4441 gagaggggct aaagccgcgt atctgtatca tcatgcacat ggtgccgggt gcctcccggt
     4501 gagttcagcc cggtgccact aaacccgcgt cattctcgtt ttgataatca gagattatac
     4561 cgtcaccagt cgcccctccg ctcaggggga ttcaccatgc gaaatttttt taacaaatgc
     4621 ccagtctgac aggcaactgt caacttactg aattgtgagc aacatagcat ttaacgggga
     4681 acctgttttc tgcagtaaaa aggcccaccg gagcggatgg gcctggaagg atagcggtca
     4741 tgtgatgccg gtttcccggt aactcagcac cggtatctga gtcaacgttt tctctactgg
     4801 gtcatttccg atacgccctg cctgctgaca ggctttcatc acatctgaaa atatagcacc
     4861 ctgactgata ctgtagtacc taaggttcca gaaactgtga tgtatccggc acagaaaagc
     4921 ccctccggag aggggctgga gagtggcgct atgtgccatt gcatggtgcc gggtgcctcc
     4981 cggtgaattc agtaccagca cctgaatccg cgattatccc atatacctac tcgctgattg
     5041 cccctccgca cagggggatt caccatgcca gtttctttta acaaactccc cgcaaaccag
     5101 acaacagtca accgcctgaa ttgtgagaca tttaaaaaaa agcccgcaaa agcgagccag
     5161 ggaaaataag tgtggcgcgt tgtactggat tcgaaccagt gaccgattgc ttagaaggca
     5221 attgctctgt ccggctgagc taacaacgca ggatacagat aatggaccgc cttcggggac
     5281 ccgaactccg cgcaaccagc ttcgaaggct ggcgctcttt cctgatgagc taatggcggt
     5341 atgtgatggt ggcccttgct ggatttgaac cagcgacctg gcgattatga gtcgctcgct
     5401 ctcaccactg agctaaaggg ccgggagcag aataataatg gtgcgtaatt aattctgcaa
     5461 tctcatccgt ttcaaacgat taaatcctga acttccctga ctgtctgctc aaaacgtccg
     5521 gtctccagtt caacgccaat cgcacgacgc ccgagcgcca gtgcagcttt taccgttgaa
     5581 cctgagccca taaaaaaaat ctgcaaccag gtctcccgga cgactgcttg cgctgattat
     5641 ctgctgcagc atttctgccg gtttttcgca cggatgtttc ccgggataga actgcaccgg
     5701 tttatgtgtc cacacatccg tgtacggcac ctgcgccgtc acaccaaaat accgccgcag
     5761 atgcttatat tcactctgca gctccacata ctgccggttc agtgacgtat acgtatccac
     5821 cagctggtgg tggggctttt ccagttcacc gcgctgatgt ttctcttctg ccacccgggc
     5881 aaacagcgac tgtaatttca gataatcgct ttcgttcggt agctgccact gactggcact
     5941 gaaccagtgc gacaccatgt ttttctttcc tgtggcatct gcaatctgtt ttgccgttat
     6001 ccccagggcc gcgcgcgcat cacgaaagta agaaatcagc ggggccatca catgctgttt
     6061 cagtgcactg cccttcgccg catacccggc atctttcgga cgatacggcc cctgataatg
     6121 ttccgcgaac agaatgcgct ctgtggcggg gaaatacgcc cgcaggcttt ccttgttgca
     6181 tccgttccag cgtccggacg gcttcgccca gataatatgg ttcagcacac tgaagcgttc
     6241 acgcatcatg atttcgatat cagatgccag gcgatgacca cagaacaggt aaagacttcc
     6301 ggcaggtttc agcacccgcc agaactgcgc cagacactgg tccagccact tcaggtaatc
     6361 atcgtcgccc ttccactggt tatcccagcc ctcaggcttc actttaaagt acggcgggtc
     6421 cgtgactatc aggtcaacag aattttcggg taacgaccgg ataaattcca ggcagtcggc
     6481 gttgattaac tcacaactgg atatttttac agtattaagc atggatcatt aagcctgtct
     6541 ctgataggct cattctgctt ttgcgcaaag cagtgggcct gaggtttgct tgtgaaccca
     6601 acgcatgagc agatggctgg tgggtgcccc taacacccac cagccgccca tttaccacaa
     6661 ataaaaaagc cttcactgcg gaaggcgtct gtaacaaccg aactgatagt ctgccagacc
     6721 cgccataacc agctgggtca gtattaactg gcagcgttcg cgtgaaaggt aagtattctg
     6781 tgcaatctcc ccgactgtcg ccggttcggt aacgcttaat tcattaaaca ccactctggc
     6841 ggtttctgtc atatcctgct gttttagcat gtctttttcc cttttccggt taacgtgaca
     6901 caccaataac tcttgtcgaa aaagccagca agctgaaaga caggtattca ccgccaccag
     6961 cgcgtttact gtactgacgc gatttcagtc ataaaaaacc cgccaggcgg cggggtgtaa
     7021 aaaatcttct aacgtcaggc ataaaacgcc catcgttaga gcaaatttac cacagattcg
     7081 ggaaaaatca acaacactat cgcgttaccc tctttaactg ccgctccgcc catgcctctt
     7141 caatgtcaaa ccgaaccacc aacgtatcgt aaaagcgttt cactgatttt ttccacgtat
     7201 caagcgtgat agcactcgtc actttgcata tggcattaaa tgcctccgtt gatggtagtc
     7261 tttcacagcc acgaccacca caacgctggc agtctctgat aacaggcata ccacgtttta
     7321 ccgactcttc acgatgaatg gcgacaccac gcccacggca atccttacag gcggtggaaa
     7381 cctcaccctt tccgccacac tccggacagg caacttttac cacctccctg acttttttcc
     7441 attcttccca gtaagacgga tacacacctt tcgtacactt tgcccatacc ggcggcttac
     7501 catccggata ctggaccttg tttgtaaaaa ctacgctttc aataaatttt tccccatagc
     7561 aacaagggca ctgcttttta ctcgctgcgc tgcgggcata atcctcaaaa gcgtacgaag
     7621 ccataatgcg catcactacc ggttttattt ctgccgggag ttttcttaac gccgccacgc
     7681 gatcacaccg actgagtgca tattctgtca gcaattctgt tgcccgctct ctgtcattca
     7741 tactaatgcc cattttccca aggaacgcag aaaaccccat ctcagcccga ttctgtgtca
     7801 tgccctgcgc ggccatcaca tcagtgatac tcagcgcatc tttcgacgtt gaggccgatg
     7861 catcagtcag gccgggggat tttggggagt agtatttcgg taaatcttcc agtttcattt
     7921 tttgacctgc ccttcaagca ttatggggta aatcttcacc cccagacgtc caccagatac
     7981 tggctgagca cgaacgatat tgatttcatc aaactgctca tcgtccatta gcaaccccgc
     8041 atgcgtcagc gcatccagcg gcgctttcag aatattgtcc aggtcacggc ggcgcttatc
     8101 cggtggttct gcaataattt ttattgccaa ccttccggac aggcttaatt tcagccgctg
     8161 ctggcgaaca ataagcgcca ctgcccggcg ataacgctcc ccggcttttg atacaaaata
     8221 tgtgctgcca cgacgacgcc agtaagtgtt caccgttggc gggtaaggca aaacaaattc
     8281 tatgcgttca gtcatttatg ctttccactt cagaacaccc gaatttctcg cgtgcattaa
     8341 aaaacgaatc agcaacaaca gctggctgcc gtgtttttct tcaaaatctt ttaccccggc
     8401 gtgtagttcg ctatggcatt tacggcacag cggaataaca aacaaatcat cagcctttgt
     8461 tcccatccct cccagtccat aaccaatgat gtgatgcgga tcatctgcct gattgccaca
     8521 cgtcatgcat ttctgcgttt ttacccaacg cgtgtataca ggcatctctt cccgttgtga
     8581 tttctggcgc tggagatact gagccggtga ctccggatca acggcaatgc tgaccaccgt
     8641 cttttcctgt ggcgggtttt gctggtgggc gtgaggcagc ggcgcaagat tttttgtgcg
     8701 ctgcttcagt atgctggtgg cggtctgctc tcccggtacg atgtcgcttt cacggtacat
     8761 tgagcggatt ttttccgcac gcaaccccag cgaacgacgt aataccgctt ccggtagcgc
     8821 gtccgccacc tgattgcgga ccgcccacca ggataattca gccagcgata attcccgttc
     8881 ctgcgagcca ttcattgcat ggcgtatgac gtcaatcatc catgcaaaca ggttttggtg
     8941 agcaagttgc ccgagtgatt cggaggtctg gtcgcgcagc tggttgtcgc agtgccagca
     9001 caacaccatt gcgccggtac cataacggtg aatgacggtt tcactgtggt gataatcgcc
     9061 gtgtggccac tggcaggatt taacatggcg cagtaaccag tcagacaatg cgccagcgcc
     9121 accagcagca cgaatcactc gttcgtcgct gaaaaatggc agtaatgatt tatcctccgc
     9181 cagcggctgg cgaacggcag gaacgacccc ggacggcaga ttacgcatgc ttttcggttc
     9241 cggctccacc agtacccggg tattgtggaa taccggcatg gattcacggc ccggcttaac
     9301 gatcaccagc ccgagttccg gtaccagaac aggtcgaagt aatacccgca cgttacctcc
     9361 agatgcgttg ctggaatgtg cgggacggac gcggtgggcg ttcagagtaa ggaagcctga
     9421 cggagattat ccagtgacga taatcgaggc tgagggcttt cttaatctcg tatccgcgtc
     9481 tgcggtagtt atgaattagc cattcggcct gttcttcagt acatggtggg tgttggtacc
     9541 agtcggtttt aaatgcgtgt gaacgccgcc catgccggat ggcaaggtcg gtatcagaat
     9601 tgtgaaattt ggttttgtgc gccatctgtt ttctctgctg gcgcagcagg tgtcaggtgt
     9661 tcaggctgac gtgcgaattg taaaccagaa tgccagaaaa aaacaaaacc cgccgaaccg
     9721 ggttaagtgc gggtgcgttg aggatgcctg acacatcaga ggtggcgagg gatttctccc
     9781 ccgccaggtc tcttactcct caggttcgta agctgtgaag acagcgacct ccgtctggcc
     9841 ggttcggatt cgtacctcgc agaggtcttt cctcgttacc agtgccgtca ctatgacggt
     9901 taaacagatg acgatcaggg cgattaacat cgccttttgc tgcttcatag cctgcttccc
     9961 cttgcctttc ggcacgtaag aggctaacct acatttgtga gacatagatt gggcctcaga
    10021 ttaatgttaa gcgtcttgca ggacgcgaaa tgttaactgg ggcttttctc tatctgcctt
    10081 tcagtgttca tgcctgagac agatagcctc aagcacccgc agccattcta cttaactcac
    10141 gtcacctcgc caatatgaaa tcaatcagaa aggtgatcca taaaatcact ccttctcttc
    10201 ttttccgtag tggagttggc caattttgat aagagggcgt ccctgagatt tgcggtgtag
    10261 attggtatcg cgcagagaat acacacagcc acaatattcc tgctgataga atttttcgcg
    10321 cttgctgatt tcaatcatac gggacgagcc gccctgcttg cgccagttat aatcccagta
    10381 caccataccc ggataatgcg caacagctcg ccgcccacac tcgttaacct gctgcatatt
    10441 tttccagcgt gaaatgccca gtgaactgct gatcacactg aaaccatttt cagcagcgta
    10501 caacgctgtc cgctcaaaac gcatgtcaaa acacatggta caacggatcc ccctctcagg
    10561 ctcccattcc attcctttgg cacgttcaaa ccagttgtcg gtgtcgtaat cagcatcgat
    10621 aaacggcacg ccgtgttgtt cagcaaagcg aatattttca tccttacgaa ttaaatactc
    10681 tttctgagga tgaatgttcg ggttgtagaa aaagatggtg tagtcgattc ccgaggcctg
    10741 aagcgcctcc atcacttcac cggaacatgg agcacagcaa gagtgcagta gtagtttgtt
    10801 tgccccgttt gggagctcca atttaggccg tttgaaatca gcaatagtca taaatatttt
    10861 tattggggtc atgaaaatag cacagagtgt agcatcagag cagggctatc gggaatatat
    10921 gtctaaatct ggtaatatct ggttttgacg caaagcggac aaccacgctg gctctaccct
    10981 gcgccatgaa aatgtcaatt cacatctgaa ctaatgctct ttaatctagt aacgtctaaa
    11041 atacctaaca tttccttgat aaaatgccag tacacgctgc atagcttcgc tcttccggca
    11101 ctcgcgacag attatattca ggcgcctgtc gtagcggcgt atttcgccgt ctggtaacga
    11161 ccagataagg tccggatcaa ccactgcagg tttcttcacc tttgcccttg agagtttttt
    11221 gcgagcattt tgccagtcct tacgcgcctg ttcagacggg aataacccgt aaccagagtt
    11281 gtatacatcg ccactggcaa ccagctctct ggcgagaaca ctcatcagat atcttgtcgc
    11341 acctgtcttg gcttccagtt gccgcaacgt ctcgcgaccg ctcagacgta caagttcaac
    11401 aacctgccct ttaatttttt cccgctcttc ttgtgtaaat acttttgcca taagcgcctc
    11461 cggcaatcac ttttccgata caacacggcg ggaagaatca gtaatctgtc gaacaatatc
    11521 ccggtgcttg ttcagctccc gcagcgcggc gcagactcgc tcccacttct gaacatcact
    11581 tttcgccctg cgcagcgcca ggtttgccct gcgaagggac ggaaaaatca gctcatctgc
    11641 ttgcgtttcg gtaaacgatg gcaacggctg cacaatgtcc gccacagttt ctgttttaat
    11701 ttcttcctgt gttgcggctt cccggactgg taacgcagca cctgctggct gaggaaaggc
    11761 cttaccatca ctttccgtta ccagcgcggc tttcggctct gctggtaaat tatcgcccgg
    11821 catgcagtaa cgaaatttac cgttctgatt aacgcgtgcc agccgccccg ttgcggttac
    11881 caccgccagc gtggaagcaa ccttgcgagt actgacaccg aacttacccg ccagttcctc
    11941 acacgtttta gccccatcct gaccgataaa ctcaatcatc atgtctgcgg taactttttg
    12001 ttcgacctcc ccggtcagca tatcctgtgc ttcagatttt actggccgct cttcggttac
    12061 ccgggattca ccttcgccag ccagaaacca ggtgtgacca gttttatcaa cgacgccttt
    12121 tcttttgagt tcccacagct cgttgacagc ctcttcacga ctgattccaa ggcgagctgc
    12181 caccacatgt gaagaggctt ttttcagtgc tttcagtgcg tcagatacgg tttccattaa
    12241 aatttcctcc ggacaaaatt acttcacaac cctcatattg ctgacatttg gacgccagct
    12301 atcccagtta aacgtcaccc atcgaccacc gttcatggtc atgcggtcca taatcctctc
    12361 accaagaagc gtactcattg cggcatgatt caggtttgtt aacatcccga cactgcacag
    12421 tgatgctgtc cggcgatcaa ttatctggtg caataccacc tgctcgtttt tcgtctcccg
    12481 ctgaacgcct atttcatcca ggaccagcaa atcaaccccg caaagctcct gtatttttcc
    12541 ccggatttgc cgttgtcgta gctgtcatgc aacacgctca tgacgtcaga cacggtgacg
    12601 ataatcacgc tgcgcccctt caccatcagc cggttgccca tcgccgctgc aaggtgattt
    12661 ttcccggtgc cggttttacc gctgaacaca aaattcgtgc acccggtcat cagttcgtca
    12721 gctatggatt tggcctggct cagcgcgtat ttttgcccgt cgttctgcac ctgataattt
    12781 gcaaacgagc atttgctgtg cagaggctgg atgcccgaac gattcaggat tttttccacc
    12841 cgcaactggc gattctggcg gttaatctcc tcgctgcgtt ttcgtccttc agcaagttgc
    12901 cattcccgcc actcctccac cgtccggtac ggtggaaccg acccctgtgg tgcaagtctg
    12961 cgaatacgtt caagaacccc aactgccgca atgtttttca tgacacgtca ccccctgaat
    13021 cccggcggta tttcagtgtc cggttcagaa atgtgattca cacaacgctg cgcaggcgaa
    13081 cgccccaggc ggataaccag ttcatcccat ttttcccgga gttttgccgg actcatgatg
    13141 ttttttaccc agaacgaatc ccgctggaga cgcccaaaca tttcacaaat ttgtctgtga
    13201 gttctgccat ccagcatccg cattgtgcga acgtcattgg cccatgctgt ccagttgggt
    13261 tctttcggtc tagtgatctc gccatcatag ctggccgcct gctcgtaaag actcacgatt
    13321 cgtccccaga tccactgtgc gcacaccaaa tcttcctgac ttccccactg gcgttttttc
    13381 gcactgaaca caaccgcgtc agggtgtcgg gttaaaaaat cctgttcagc cgtctgcggg
    13441 tccggttgcg aagcgtccgg acaagaagat cttttatctg acggatcagg ttttaatact
    13501 gacggatcgg ggtcaatcat cgccccccta atcggcagtt ttttatcaac agttgatcca
    13561 tcaaaatttg acgggtcaac cgttgagggg tcaatatttg acgggtcaac tgttaacggg
    13621 tcattttttg ccgggctaat ttttcttttc ggtttatatg actcacgcgc cgccgccgca
    13681 gctgcttcga gtttttccac attaagccga tagatattgc ttacattacg cccaccgacc
    13741 ttacgctctt ccttcgtcag ccagccctct ttcgccagtt ctgcaatagc cgatttcact
    13801 gtggattcac ttcttgcacc gatctgacgc cggatagttt caatggcagg ccatgacacg
    13861 ccctcgtcat tgctgtagtc tgcaagacgg gccataaccg ccaccctgga taagatcatg
    13921 ccggtgaagg cgcacccttc ccagacaaga ccatgaagct tgctgctcat aaaacccccg
    13981 aacaccgtgc ttttagtgca tcaccacagc attccctgcc gggccgccgc gattcatctg
    14041 gtcatacaaa acaaccgctg acgcaacaaa atcatcgaca tccttcacca gccgatccct
    14101 ccgttcgacg atctcacggt aatattcaga actgtggctg cgcatacggg ccaccagcaa
    14161 aggcggcatc gccttttcga tcgccggtaa cagagcctgc attttttcaa cagcatcagg
    14221 ggggtcttta tccagccaac ggaaaatttt ctgggtatta cgggccaggg cttccggatg
    14281 gctgtcgtcg tacagttcag ggaacgtcat ccccagttcg aaataagtcc gggctatttc
    14341 agctgcagga actttctcac catcagggta tgcccaggca ttcatcgcca tgcggatgtg
    14401 ctcatgtttg attttcatga atcatttgcc tcttgatgct tcgggtatga tcgttttcgt
    14461 catttggttg cttcatcgac atattctgcg aataacatga cgagcgtcgt aagtatgtcc
    14521 aatcaacatc aggacgaagt tcttcacaca ggacaccacc ttttgttgct cgttcaatcg
    14581 caggacatct ctcagcaggt aactgacgta cacctttgat ccattgattt acgcttggag
    14641 gagatacacc taaaagccta gccattgctg attgcccacc gacaacagca caagctcgtt
    14701 tgaatgaata gttatctttt ttcatcgaat gaactccaaa aaacacgcaa caatattagg
    14761 cttagcctaa tgaaattgtc aataggctat gcctaataca tcgagagtag ggattgccta
    14821 acgcgatgcg cataggagac tattaagcaa tgcttagtgg taaagactta ggccgagcga
    14881 tagagcaggc cattaacaaa aaaattgcat caggagccgt caaatcaaag gcggaaatcg
    14941 cacgtcattt caaagtccaa ccaccatcaa tccatgactg gattaagaaa ggttcgataa
    15001 gtaaagacaa acttccagaa ctatggcgtt tcttttctga tgtggttggt ccagagcatt
    15061 gggggcttaa cgaatacccc ataccaaccc catccacttc agatacaaaa agtgaacttt
    15121 tagacataaa cagcctttat caagccgcct ctgatgaaaa aaaagcaatt gtggctttca
    15181 tcttatctgg aaatgctacg gagcctagtt gggttgatta tgacgttcgc gcctacattg
    15241 ccgcaatgga aatgaaggta gctaactatc tgaaaaatca agaatcaaaa cggaaaagcc
    15301 agaacatcac caagacagga acttaaactt atatggtccg acgggaaatt cctagttccc
    15361 gttagttaac tcctactacc tctcccacaa accatcacct attaggttgc gcccaaatta
    15421 ttaggcatag cctattgaca agtaattagg catttcctat agttttccca taccaaccca
    15481 tcccgtccca cacaatacag ggcaatacct cgagttacca ggcagtggtc aggggttaag
    15541 tagccagccc gaggcgtaag aacatgacgg cagggttcaa ctttaataac tatgcagcag
    15601 gtttttgttc cgctaccccg gcgttaaggg gaaatgaggt cagcatggat actatcgagc
    15661 ttggcaacag cgaatctctg gtatgtggcg tgttccccaa ccaggacggt acgttcaccg
    15721 cgatgacgta taccagaagc aaaacgttta aaactgaagc tggcgcgcgt cgctggttaa
    15781 ccagaaacac tgactgatga ggttgacgat ggaatttaaa gatttaccag taccattcca
    15841 ggaaatggca tcgaatgtgg ttcgctctca actggcgact cttgacctga gtaccgtaga
    15901 aaaagaaacc atcgacaata tatccggtaa cgtacgccga gcctttatcg ggctgtacga
    15961 agagaagctg ctctctgata accaggattt acatgaaaaa tacttcctgg atctaatgga
    16021 catcattgat aaggggtttg gcttgttaat gaaaaagaaa gggattcgaa tagaacccct
    16081 tgaaaattac tttgcaacaa aaagcattaa ttcttttgat tcaaaataag agaattaatt
    16141 acagacttaa catgctcttt ctcatgattg aagctctcat gattgaaagt gccgggttga
    16201 agcgagtcga tataatcaac aagactctgt cgtacgactt catttttatc cataacagat
    16261 gcaagaaatg aaattgctaa aagagttata tcactacgcg ccgcagcatg ttgcaatgct
    16321 ttatcaaaat tattaatctg gcgtatcagg gagttaatga tttcatcatt ttcagtcgac
    16381 atttcaccct cctgagggtt ggtgattaag gagttctcca cgggtgaggt ggagtgcgtg
    16441 cgccggacac gggtgagcat ccggcactga cagtttactg aaaggatatt tccctgaaaa
    16501 gtcagaccat aacgcgaaag cgcccggcga ggtagctggt tcatagatag cctgtcgtta
    16561 aattttcgtc gaccgtgcgt ttccggttgt ggcaatccgc gaaatggcgc ggcggtaagt
    16621 atggcggggt tattccttcc ccgttgagga caccgagttg tcaggttgac catacgctta
    16681 agtgacaacc ccgctgcaac gccctctgtt atcaattttc tggtgacgtt tggcggtatc
    16741 agttttactc cgtgactgct ctgccgccct ttttaaagtg aattttgtga tgtggtgaat
    16801 gcggctgagc gcacgcggaa cagttaaaac caaaaacagt gttatgggtg gattctctgt
    16861 atccggcgtt aattgttaac tggttaacgt cacctggagg caccaggcac cgcatcacaa
    16921 aattcattgt tgaggacgcg ataatggaaa cgttattacc aaacattaat acgtctgaag
    16981 gttgttttga aattggtgtc actatcagta accctgtatt tactgaagat gccattaaca
    17041 agagaaaaca cgaacgggag ctattaaata aaatatgcat tctttcaatg ctggcccgtt
    17101 tacgtccgat acaaaaagga tgctggcaat gaatacagca tttgcacttg ttctgacagt
    17161 ttttcttgtt tccggagagc cagttgatat tgcagtcagt gttcacagga caatgcagga
    17221 gtgtgtgact gcagcaaccg aacagaaaat tcccggtaac tgttacccgg tcgataaagt
    17281 tattcaccag gataataacg aaatcccggc aggtctttaa aacagttccg taataaatat
    17341 ccggtttcat tcttatatgc cagcaatggc agggatttgt tcacccttaa atctgtaatg
    17401 aggtaaaaca aaatgagtaa agtctttatt tgcgccgcca ttccggacga acaggcaata
    17461 aaggaagaag gtgccgtcgc tgtagccact gccattgaag ccggtgatga acgtcgcgcc
    17521 cgcgcaaaat ttcactggca attcctggaa cattatccgg ctgctcagga ctgcgcttat
    17581 aaatttcttg tctgcgagga taaacccggt ataccccgcc ctgccctcga ttcctgggat
    17641 gctgaatata tgcaggaaaa ccgctgggat gaggagtctg cttcctttgt cccggttgag
    17701 actgaatcag atccgatgaa cgtcactttt gacaagctgg cccctgaagt acagaacgct
    17761 gtcatggtta agttcgacac atgtgaaaac atcaccgttg atatggttat tagcgcacag
    17821 gaattgttgc aggaagacat ggcaacattc gacggacata tcgttgaagc gttgatgaaa
    17881 atgccagaag ttaacgccat gtatccggag cttaagctgc atgccatcgg gtgggttaag
    17941 cataaatgta agcctggtgc caaatggccc gaaattcagg cagagatgcg catctggaaa
    18001 aaacgtcgcg aaggtgaacg caaggaaacc ggaaaataca cgtctgttgt tgatctcgcc
    18061 cgcgccagaa ccaatcaaca gcacagtgaa aattcaacag gaaaaatcag cccggtcatt
    18121 gctgccattc atcgcgaata caagcagaca tggaaaacac tggatgacga actggcctac
    18181 gctctctggc ctggtgatgt ggatgccgga aacattgacg gcagcatcca tcgctgggca
    18241 aaaaatgaag ttatcgacaa cgaccgcgaa gactggaagc gtatctcggc atcaatgcgc
    18301 aaacagcctg atgcccttcg ctacgaccgc cagactattt ttggccttgt ccgtgaacgt
    18361 ccgatcgaca ttcacaaaga ccctgtggca ctgaacaaat acattactga atacctgact
    18421 acaaagggcg tgtttgaaga tgaaggaaga aatcagagcg caactgatac tctctcgtcg
    18481 ccagtaccag aaactgatgc agtggaaacg gcaattccgg gcaacgaaaa aaccgaatgc
    18541 aaagtggaag tcgaaccatc tgtagagcgt gaggggccgt tctacttcct cttcaccgac
    18601 aaggatggcg aaaaatacgg tcgcgcaaac aaactttctg gtctggaaaa agcactagcc
    18661 ttgggagcta cggaaatcac aaaagaggaa tacttcgcac gtaaaaacgg cacgtactca
    18721 ggttcacaac aaaatactgg tgcatctgac acgaccgcac aaccagagcc ggtaaaagtt
    18781 accgctgacg aagtaaacaa aattatgcag gcagccaata tcagccagcc tgacgccgat
    18841 aagttgcttg ctgcctctcg cggagaattt gttgcaggga ttagcgaccc gaatgatccg
    18901 aaatgggtaa aggggattga aacccgcgat tctgtaaacc agaaccagca agaatcggaa
    18961 cagaacgacc agaaagcgga acaaaacagc ccaaatacgc aacaaaacga gccagaaacg
    19021 aaacaacctg agccagtagc gcaacaggaa ccggaaaaag tctgcaccgc ctgcggtcaa
    19081 agcggtggcg gcaactgccc tgattgtggc gcggtgatgg gtgacgcaac ataccaggaa
    19141 acattcgatg aagagaatca ggttgaagtt caggaaaatg atccgaagga aatggaaggc
    19201 gctgaacatc cacacaagga gaatgctggt agcgctcagg atcacgccag cgatagtgaa
    19261 actggcgaga cggcagatcc cttaattgcg atgaacggtc atcacgttat cacatccacc
    19321 agcaggacgt gtgaccatct aatgatcgac cttgaaacca tgggaaaaaa tcctgatgcc
    19381 ccgattatct caataggtgc aatatttttc gatccgcaaa ccggagatat gggaccggaa
    19441 tttagtaaga ctatcgatct ggaaactgct ggcggagtca ttgatcgtga cgtcattaaa
    19501 aggtggctga agcaatcacg tgaagcgca
//
LOCUS       DAJTMC010000072        19488 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.072, whole genome shotgun sequence.
ACCESSION   DAJTMC010000072 DAJTMC010000000
VERSION     DAJTMC010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19488
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.072"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            <1..1230
                     /locus_tag="OO130_002233"
     CDS             <1..1230
                     /locus_tag="OO130_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HCR5365629.1"
                     /translation="FFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRSS
                     LESAWKYIIICTVDVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLML
                     LAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIIIC
                     QAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGPL
                     GIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGALA
                     LAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQA
                     VNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTWH
                     DFLPSGTASVSEKHSER"
     gene            1220..2935
                     /gene="hyfG"
                     /locus_tag="OO130_002234"
     CDS             1220..2935
                     /gene="hyfG"
                     /locus_tag="OO130_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
                     /protein_id="HCR5365630.1"
                     /translation="MNVNSSSNSGEAILAALKTQFPGAVLDEERQTPEQVTITVKINL
                     LPDVVHYLYYQHDGWLPVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSR
                     EFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDDWPEDMHPLHKDAMDYRL
                     RPEPTTDSETYAFINEGNSDARVIPVGPLHITSDEPGHFRLFVDGEQIVDADYRLFYV
                     HRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSI
                     LLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELLTGSRKTYGLNLIGG
                     VRRDILKEQRLQTLKLVREMRADVSELVEMLLATPNMEQRTQGIGILDRQIARDYSPV
                     GPLIRGSGFARDLRFDHPYADYGNIPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFA
                     LDNMPDTPLLTEGFSYKPHAFALGFVEAPRGEDVHWSMLGDNQKLFRWRCRAATYANW
                     PVLRYMLRGNTVSDAPLIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIERYGIDRN
                     RSPLK"
     gene            2945..3484
                     /gene="hyfH"
                     /locus_tag="OO130_002235"
     CDS             2945..3484
                     /gene="hyfH"
                     /locus_tag="OO130_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HCR5365631.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACPANALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTNKA
                     DLYTRATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLRAQASVCPECKQ
                     RATLINDDTDVPLVAKEQL"
     gene            3481..4239
                     /gene="hyfI"
                     /locus_tag="OO130_002236"
     CDS             3481..4239
                     /gene="hyfI"
                     /locus_tag="OO130_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HCR5365632.1"
                     /translation="MSPVLTQHVSQPITLDEQTQKMKRHLLQDIRRSAYVYRVDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            4232..4645
                     /gene="hyfJ"
                     /locus_tag="OO130_002237"
     CDS             4232..4645
                     /gene="hyfJ"
                     /locus_tag="OO130_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HCR5365633.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHRSQVMYYSLAIGHHVGV
                     IDCLNVAFRCPLTENEDWLAQVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFALLA
                     DDATSVWQARSIQFIHLLDEIVQEPAIYLMARKIA"
     gene            4675..6687
                     /gene="hyfR"
                     /locus_tag="OO130_002238"
                     /pseudo
     CDS             4675..6687
                     /gene="hyfR"
                     /locus_tag="OO130_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416986.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
     gene            6709..7554
                     /locus_tag="OO130_002239"
                     /pseudo
     CDS             6709..7554
                     /locus_tag="OO130_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter"
     gene            complement(7592..8653)
                     /locus_tag="OO130_002240"
     CDS             complement(7592..8653)
                     /locus_tag="OO130_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR5365634.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            8866..10329
                     /gene="bepA"
                     /locus_tag="OO130_002241"
     CDS             8866..10329
                     /gene="bepA"
                     /locus_tag="OO130_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HCR5365635.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADNADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHLTQRHLARAMEDQQRSTPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            10350..10709
                     /gene="arsC"
                     /locus_tag="OO130_002242"
     CDS             10350..10709
                     /gene="arsC"
                     /locus_tag="OO130_002242"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HCR5365636.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLANSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(10847..11593)
                     /gene="hda"
                     /locus_tag="OO130_002243"
     CDS             complement(10847..11593)
                     /gene="hda"
                     /locus_tag="OO130_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HCR5365637.1"
                     /translation="MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(11643..12932)
                     /gene="uraA"
                     /locus_tag="OO130_002244"
     CDS             complement(11643..12932)
                     /gene="uraA"
                     /locus_tag="OO130_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HCR5365638.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEEADITDK"
     gene            complement(13018..13644)
                     /gene="upp"
                     /locus_tag="OO130_002245"
     CDS             complement(13018..13644)
                     /gene="upp"
                     /locus_tag="OO130_002245"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HCR5365639.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            13968..15005
                     /gene="purM"
                     /locus_tag="OO130_002246"
     CDS             13968..15005
                     /gene="purM"
                     /locus_tag="OO130_002246"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HCR5365640.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            15005..15643
                     /gene="purN"
                     /locus_tag="OO130_002247"
     CDS             15005..15643
                     /gene="purN"
                     /locus_tag="OO130_002247"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HCR5365641.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDVTARVQTQEHAIYPLVISWFADGRLKIHENAAWLDGQRLPPQGYAADE"
     gene            15815..17881
                     /gene="ppk1"
                     /locus_tag="OO130_002248"
     CDS             15815..17881
                     /gene="ppk1"
                     /locus_tag="OO130_002248"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HCR5365642.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNPEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPHLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            17886..19427
                     /gene="ppx"
                     /locus_tag="OO130_002249"
     CDS             17886..19427
                     /gene="ppx"
                     /locus_tag="OO130_002249"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HCR5365643.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRVEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
BASE COUNT         4703 a         5093 c         5093 g         4599 t
ORIGIN      
        1 ttcttccatc tgtttttgtt caccatgctg ctggttgtta ccagcaataa cctgattgtg
       61 atgtgggcgg cgatcgaagc caccacctta agctcggcgt ttctggtagg catttacggt
      121 cagcgttcat cgctggaatc agcatggaag tacatcatta tttgtactgt tgatgtcgct
      181 tttggtctgt tcggtaccgt gctggtatac gccaacgccg ccagcgttat gccgcaggca
      241 gaaatggcga tattctggag cgaggttctt aagcaatcgt ccttgcttga cccaacatta
      301 atgctgttgg cctttgtgtt tgtgctaatt ggctttggta ccaaaaccgg gctatttccc
      361 atgcacgcct ggctgccgga tgctcacagt gaagcgccga gtccggtcag cgccctgctc
      421 tccgccgtat tgctgaactg cgcgctgttg gtgctgattc gctattacat cattatttgc
      481 caagccatcg gcagcgattt ccccaaccgg ttgttgctca tcttcggcat gttgtcggtt
      541 gccgtggcgg catttttcat tctggtacag cgggacatta agcgtctgct ggcgtactcc
      601 agcgtggaga acatggggct ggtcgcggtg gcgctaggca ttggcgggcc gctgggaatt
      661 tttgccgcgc tgctgcacac cttaaaccac agtctggcaa aaacgctgct gttctgcggt
      721 tccggcaatg tactgctcaa gtacggcacg cgcgatctca acgtcgtctg tgggatgctc
      781 aaaatcatgc catttaccgc cgtgctgttt ggcggcggtg cgctggcgct ggcagggatg
      841 ccgcccttca acatttttct tagcgaattt atgaccgtta ccgcaggact ggcacgtaat
      901 cacctgctga ttatcgtcct gctgttattg ctgttaacgc tggtgctggc gggcctagta
      961 cggatggctg cgcgggtgtt aatggcgaaa ccgccgcagg ccgttaaccg gggtgatctc
     1021 ggctggttga ccacctcgcc aatggtgatt ctgctggtca tgatgctggc gatgggaacg
     1081 catattccac aacctgtcat caggatcctg gcgggcgctt ccactatagt cctctcaggg
     1141 acgcacgacc tgcctgcaca acgtagcacc tggcatgatt ttttgccttc aggcaccgca
     1201 tctgtttcgg agaaacacag tgaacgttaa ttcatcgtca aatagtggcg aagcgattct
     1261 cgccgccctg aaaacgcagt tccccggcgc ggtactggat gaagagcgac aaacgcctga
     1321 acaggtcacc attacggtga aaatcaatct gctgcctgac gttgtacatt atctttatta
     1381 tcaacatgat ggctggcttc cagtcctgtt tggcaacgac gagcggacac ttaacggtca
     1441 ttacgcggtt tattatgccc tttcaatgga aggggccgaa aaatgctgga tcgtggtgaa
     1501 ggcactggtc gatgccgaca gtcgggagtt tccgtcagtc acaccgcgcg tccctgccgc
     1561 ggtctggggc gagcgagaaa ttcgcgatat gtacgggctg attccggttg gcctgccgga
     1621 tcagcgtcgc ctggtgttgc ccgatgactg gccggaagat atgcatccgc tgcacaaaga
     1681 tgcgatggat tatcgactgc gccctgaacc gacgacagat tccgaaacgt atgcgtttat
     1741 caatgagggc aacagcgatg cgcgggtgat ccctgtcggc ccgctgcata tcacctccga
     1801 tgaaccgggt cacttccgct tgtttgtgga tggcgagcaa attgtcgatg ctgattaccg
     1861 cctgttttat gtccatcgag gcatggagaa actggcagaa acgcggatgg gctacaacga
     1921 agtgaccttc ttatcggacc gcgtgtgtgg gatttgcggt tttgcccaca gtgtggccta
     1981 taccaactcg gttgaaaatg cactggggat tgaggtgccg caacgagcgc ataccattcg
     2041 ctcgattctg ctggaagtcg aacggctaca cagtcatttg ctcaaccttg gcctctcctg
     2101 ccattttgtt ggttttgata ccggctttat gcaatttttc cgcgtgcggg aaaagtcgat
     2161 gacgatggcg gaattgctga ccgggtcgcg taaaacctac ggtctgaatc tgattggtgg
     2221 tgttcgccgc gatattctca aagagcaacg tctgcaaacg ctgaaactgg tgcgcgagat
     2281 gcgcgccgac gtgtcggagc tggtagagat gctgcttgct acgccgaata tggaacaacg
     2341 cactcagggc attggcattc tcgaccgaca aatcgcccgt gattatagcc ctgtagggcc
     2401 gctgatccgc ggcagtggtt ttgcccgtga tttgcgcttt gatcacccct acgccgacta
     2461 cggcaatatt ccaaaaacac tgtttacctt caccggcggc gatgttttct cccgcgtgat
     2521 ggtccgtgtc aaagagacgt ttgattcgct ggcaatgctg gaatttgccc tcgacaacat
     2581 gccggatacc ccactgctga ccgaaggctt tagctataaa cctcacgcat tcgcgctggg
     2641 ctttgttgaa gcgccacgcg gtgaagacgt gcactggagc atgctcggtg ataaccaaaa
     2701 attgttccgc tggcgctgcc gtgccgccac ctacgccaac tggccggtgt tgcgttacat
     2761 gctgcgcggc aataccgttt ctgacgcacc gctgattatc ggtagccttg atccctgcta
     2821 ctcctgtacc gaccgtgtga cgctggtaga tgtgcgcaag cgccagtcaa aaaccgtgcc
     2881 gtataaagag atcgaacgct acggcattga tcgtaaccgt tcgccgctga agtaaggaca
     2941 gaagatgctg aagttactga aaactattat gcgcgccgga accgcgacgg tgaaatatcc
     3001 cttcgcgcca ctggaggtca gcccaggctt tcgcgggaaa ccggacctga tgcccagcca
     3061 atgtattgcc tgcggcgcct gtgcttgtcc ggcaaatgcg ctgactatcc agaccgacga
     3121 ccagcaaaat tcgcgcacct ggcagctcta tctggggcgt tgtatttact gcggacgttg
     3181 tgaagaagtg tgcccgacca gagccatcca gcttaccaat aactttgaac tgaccgtcac
     3241 caataaagcc gatctctata cccgcgcgac gttccatcta caacgttgca gccgttgcga
     3301 acgcccgttt gccccgcaaa aaaccgtcgc actggctgct gaattgttgg cacagcaaca
     3361 aaatgcgcca caaaaccgcg aaatgttgcg ggcgcaagcg agcgtctgcc cggagtgcaa
     3421 acaacgcgcg acgctgatca acgacgatac agatgtaccg ctggtggcta aggagcagct
     3481 atgagtccag tgcttacaca acatgtcagc cagcccatca cgctggatga gcaaacgcaa
     3541 aagatgaagc ggcatttgct acaggatatc cgtcgctcag cctacgttta tcgcgtcgat
     3601 tgcggcggct gcaacgcctg tgaaatcgaa atttttgctg ccatcacccc cgtattcgac
     3661 gcagaacgtt ttggcattaa ggttgtttca tcaccgcgtc acgccgatat tttgttattt
     3721 actggcgcag tcacccgggc gatgcgtatg cctgcacttc gggcgtatga gtctgccccc
     3781 gatcataaaa tctgtgtttc ctacggcgcg tgcggtgtcg gcggcggtat tttccacgat
     3841 ctctacagcg tctggggcgg cagcgacacc attgtcccca ttgatgtttg gatccccggc
     3901 tgcccgccaa caccggccgc caccattcac ggtttcgccg tggcgctcgg tttgctgcaa
     3961 cagaagattc acgctgtgga ttatcgcgat cccaccgggg tgactatgca accgttgtgg
     4021 ccacagatcc cgccatcaca gcgtatcgcc attgagcgag aagcgcggcg gctggcgggc
     4081 tatcgtcagg ggcgagaaat ttgcgatcgg ctcctgcgcc atttaagcga cgatcctacc
     4141 ggaaatcggg ttaacacctg gttgcgcgat gccgacgatc cacgtctcaa tagtatcgtt
     4201 cagcaactct ttcgcgtact ccgggggtta catgactgaa gagtgcgggg aaattgtttt
     4261 ctggacgctg cgaaaaaagt ttgtcgccag tagcgacgag atgccggaac accgctctca
     4321 ggtaatgtat tactcgctgg ctatcggcca tcacgttggc gtgattgatt gtctgaatgt
     4381 cgccttccgc tgcccactga cggaaaacga agattggctt gcacaggtcg aagaggagca
     4441 agcccgacgt aagatgctgg gggtgatgac ctttggtgag attgttattg acgccagcca
     4501 caccgccctg ttgacccggg cattcgcgct actggcagat gacgcgacgt ctgtgtggca
     4561 ggcgcgtagc attcaattca ttcatctgtt ggatgaaatt gtgcaggaac cggccatcta
     4621 tctgatggcc agaaaaattg cgtgagaagg atttctcatt aataaggact gttgatggct
     4681 atgtcagacg aggcgatgtt tgccccgcca caaggaataa caattgaagc ggtaaacgga
     4741 atgctcgcgg agcggttagc acagaaacac ggtaaggcgt ctttattacg cgccttcatc
     4801 ccgatgccgc cgccgttcag cccggtacaa cttattgaac tgcatgttat caaaagcaac
     4861 ttctattacc gctaccatga tgatggcagc gatgtgacgg caacaacaga gtatcagggc
     4921 gagatggtcg attattcgcg tcacgccgtc cttctcggca gtagtggaat ggcggagcta
     4981 cgctttattc gtacccacgg cagccgtttt actccctagg attgcacact gtttaactgg
     5041 ctggcacgca ttatcactcc ggttctgcaa tcatggctca atgatgaaga acaacaggtg
     5101 gcgctgcgtt tgctggagaa agatcgcgat catcatcggg tactggttga tattactaat
     5161 gcagtgctgt cacatcttga tctcgacgat ctgatcgctg acgtcgctcg tgagatccat
     5221 cattttttcg gtctggcttc agtcagtatg gtactgggcg agcatcgaaa gaacgagaag
     5281 tttagcctgt ggtgcagcga tctttctgcc tcacattgtg cgtgtctgcc acgcaatatg
     5341 cctggcgaca gtgtattgct gacacaaacg ctacaaaccc gacaaccgac cttgacgcac
     5401 cgtgcagacg atctgtttct ctggcaacgc gacccgttat tactcttact tgcatctaac
     5461 ggctgcgaat ctgcgctcct tataccgctt acctttggca accatacacc gggtgcattg
     5521 ttgctggcgc atacctcttc cactctcttt agtgaggaaa actgccagct actacaacac
     5581 atagccgatc gcatcgctat tgccgttggc aatgccgatg cctggcgtag catgaccgat
     5641 ttgcaggaaa gtttgcagca agaaaactac cagcttagcg agcagctcct ttcgaatctg
     5701 ggcatcggtg acattatcta tcaaagccag gcaatggaag acctgctcca gcaggtagat
     5761 attgtagcga agagcgacag tacggtgttg atttgtggtg aaaccggaac cggcaaagag
     5821 gtgatcgcca gagcgctcca tcaacttagc acgcgacgcg acaaaccgct ggtcaaaatc
     5881 aactgcgctg ccatccccgc cagtcttctg gaaagtgagt tattcggtca tgacaaaggg
     5941 gcgtttactg gtgcgattaa tacacatcgt ggtcgttttg aaattgccga tggcggcacg
     6001 ttgtttctcg atgaaattgg cgatctgccg ttagaacttc agcctaaact gctgcgcgta
     6061 ttgcaggaac gggagattga gcgtctcggc gggagtagaa cgatcccggt gaatgtcaga
     6121 gtcattgccg ccaccaaccg tgatttgtgg caaatggttg aagatcgcca gtttcgcagc
     6181 aatctctttt atcgcctgaa tgtcttccca ttggaattgc cgccgctacg cgaccgtccg
     6241 gaagatatcc ctcttttagc aaaacatttc acgcaaaaaa tggcgcgcca tatgaatcgc
     6301 gcaattgacg ccatcccgac cgaggcgcta cgccagttga tgtcgtggga ctggccgggc
     6361 aacgtgcacg agctggaaaa cgtgattgag cgagcggtac tgctgactcg cggtaacagt
     6421 ctgaatttac atcttaatgt ccgacaaagc cgtttactgc cgacgctaaa tgaagattca
     6481 gcgcttcgca gttcaatggc gcagttgctg cacccgacga cgccagagaa tgacgaagaa
     6541 gaacgtcagc gcattgttca ggtattgcga gaaaccaatg gcattgttgc cgggccccgt
     6601 ggcgcggcga cacgattagg gatgaagcgc accacgctgc tgtcacgaat gcagcgtctg
     6661 gggatctcgg ttcgcgaggt gttgtaatct gcttttgcag gagtatgcat gagaaacaaa
     6721 ctctctttcg acttgcagtt gagcgccaga aaagcggcaa tcgctgaacg gattgccgtc
     6781 cataaaattg cccgcagtaa agtgtcggtc tttttaatgg cgatgtccgc tggcgtgttt
     6841 atggcgatcg gatttacttt ttacctttcc gttatcgccg atgccccgtc ttcacaggca
     6901 ttaacccatc tggtgggcgg cctttgcttt acactcggct ttattttgct ggcggtttgc
     6961 ggcacagcct gttcacctcg tcggtaatga cggtgatagc aaaaagtcgg ggcgttatta
     7021 gttggcgaac ttggctgatt aacgcacttc tggtggcctg tggtaatctg gcagttattg
     7081 cctgtttcag tttgttaatc tggttttccg ggctggtgat gagtgaaaac gcgatgtggg
     7141 gagtcgcggt tttacactgc gccgagggca aaatgcatca tacatttact gaatctgtca
     7201 gcctcggcat tatgtgcaat ctgatggttt gcctggcgct gtggatgagt tattgcgggc
     7261 gttcgttatg cgacaaaatc gtcgccatga ttttgcccat caccctgttt gtcgccagtg
     7321 gctttgagca ctgtatcgcc aatttgtttg tgattccgtt cgccattgcc attcgccatt
     7381 tcgccccccc ccttctggca actggcgcac agtagcgcag acaattttcc ggcactgacg
     7441 gtcagccatt ttattaccgc caatctgctc ccggtgatgc tgggtaatat tatcggcggt
     7501 gcagtgctgg tgagtatgtg ttatcgggct atttatttac gtcaggaatc ctgaaaaatc
     7561 agcccggcga aacagttcgt cgggctgaag attattcttg cgcgatttgc ccatcgggcc
     7621 aggcgtggat cacggctttg atcagcgtcg ccaatggaat ggcgaagaat acgccccaga
     7681 atccccacaa accaccgaag atcaccaccg ataaaataat caccagcgga tgcaggttaa
     7741 ccgcttcgga gaacaacacc ggtactagca ggttgccgtc cagcgcctga ataatcagat
     7801 acaccgcgaa gcagctccag aattccgtgc ctgcgccaaa ctggaatagc gccacgccaa
     7861 ccaccggaat ggtcaccaca aatgcgccaa tgtacggaat aagaaccgag aagccgacca
     7921 gcaccgccag cagcagcgaa tagttcagcc caaagagcaa gaaccccagc caggtggcga
     7981 tccccaccac gatcatctcc agcactttgc cgcggatata gttggtgatt tgttgattca
     8041 tctccttcca cacctgtcct gccagtccac ggttgcgcgg cagcacccga cgaacggcgt
     8101 tcagcatctg ctctttgtct ttcagcagga agaagaccat caatggcacc agcaccagat
     8161 agacggcaat ggtcagcaaa ccgaccagcg aggcgaggga aattttcacc accgaatcgc
     8221 ccatggtcag catccgactg cgcatatttt cggccattgc atcaataatg cccgcgtcca
     8281 ttaacgccgg atagcggcgc ggcaacgtgg cggcaaagtc agaaagctta ttgagcatcc
     8341 ccggcatatc gcggattaag tagatgccct gttgccaggc gataggcagt accacgaacg
     8401 ccatcagtag caatataccg acaaaaacca ccaatacaat cgacgtcgcc cagcggcggg
     8461 agcagccaat agattgcagg cgcacggttg gccattccag caaataggcc agcacaatag
     8521 ccaccagcaa cggagcaagc aggccactaa agaaaaagat aatgccaaat ccggcaacta
     8581 aaataaccag caaggcaatc gcttccgggt cgctaaaacg gcggcgatac cattgcatca
     8641 acatttcgag cataaaaacc ttccctgaac ctcaagagcg ggattgcgat ccgcaattgt
     8701 atcgaaatgt cacaaaaaag acttcgcttt ttatgacgga ttcaggaaac tgaaaagtca
     8761 tttgagtggg ctaatcttcg ccgttacact caaaggcggc gcggtgggaa cgatatttca
     8821 cagtatcggt caaatgacta tcttcagaaa tacaggatag aggttatgtt caggcagttg
     8881 aaaaaaaacc tggttgcaac cctcattgct gctatgacca ttggtcaggt agccccggcg
     8941 tttgccgaca acgcagacac cttgccggat atgggaacct ccgcaggaag cacgctttcc
     9001 attggtcagg aaatgcagat gggcgactat tatgtccgcc agctacgcgg cagcgcgccg
     9061 ttaattaatg acccgctgtt aacgcaatat attaattcgc tggggatgcg tctggtttcg
     9121 catgccaatt cggttaagac accgtttcat ttttttctaa tcaacaacga cgaaattaac
     9181 gcctttgctt tctttggcgg caacgtggtg ctgcactctg ccctgttccg ttattccgat
     9241 aacgaaagtc aactggcttc agttatggcg cacgaaatct cccacctcac ccaacgtcac
     9301 ctggcgcgag cgatggaaga tcagcagcgc agcacgccgc tgacctgggt cggcgcgtta
     9361 ggttctattt tactggcgat ggccagtccg caggcgggga tggcggcgct gaccggtaca
     9421 ctggcgggaa cgcgtcaggg gatgatcagt ttcacccagc aaaatgaaca ggaagcggac
     9481 cgcattggta ttcaggtgct gcaacgctcg ggattcgatc cgcaggcgat gccaaccttc
     9541 ctcgaaaaat tactcgatca ggcgcgttac tcctcgcgcc cgccggaaat tttattgact
     9601 cacccgttgc cggaaagtcg tctggcagat gcccgcaacc gtgctaatca gatgcgaccg
     9661 atggtggtgc agtcgtcgga agatttctat ctggcgaaag cgcgcacact ggggatgtat
     9721 aattccggac gtaaccagct caccagtgat ttgctggatg aatgggcgaa aggaaacgtt
     9781 cgtcagcaac gagcggcgca atatggtcgt gctttacagg cgatggaagc caataaatac
     9841 gacgaggcgc gtaaaacgct gcaaccgtta ctggcggcag aacctggcaa cgcatggtat
     9901 ctcgatctgg ctaccgatat cgatcttggg caaaacaaag ccaatgaggc aatcaatcgc
     9961 ctgaaaaatg cccgtgattt gcgcaccaat ccggtgttgc agctcaacct ggcaaacgct
    10021 tatctgcaag gcggtcaacc acaagaagcg gccaatattc tcaatcgcta cacctttaat
    10081 aataaagatg acagcaacgg ctgggattta ctggcacagg cggaagccgc gctaaataac
    10141 cgcgatcagg agctggctgc gcgagcagaa ggttatgcgc tcgccggacg actcgatcag
    10201 gccatttcgc tgttgagtag cgccagttcg caggtgaaat taggcagcct gcaacaagcg
    10261 cgttacgatg cgcgcatcga ccagttgcgc cagctgcagg aacgctttaa gccttatacc
    10321 aagatgtaat tcgcattaaa ggaagaacaa tgaccaaaca ggtaaaaatc taccataacc
    10381 cacgctgttc aaagagccgg gaaacgctga acctgctgaa agaaaacggc gtggagccag
    10441 aagtggtact ctaccttgag acacccgccg atgcggcaac gctgcgcgat ttgctgaaaa
    10501 tgctggggat gaatagcgcc cgtgaattga tgcgccagaa agaggatctt tataaagaac
    10561 tgaacctggc taacagttcg cttagcgaag aggcgctgat tcaggcaatg gtggataatc
    10621 cgaagctgat ggagcgcccg attgtggtcg cgaatggcaa agcgcggatt ggtcgtccac
    10681 cggagcaggt actggaaatc gtgggttaag atgtgctcca tcgctagttg aagcacattg
    10741 ccggatgcga cgcttgccat gtcttatccg gcctacgaat actatggatt aacttcgcgt
    10801 agttcggata aggcgttcgc gccgcatccg acaataaaca ccttatctac aacttcagaa
    10861 tttctttcac aaacggaatg gtcagcttac gttgtgcggt aatcgacgca cgatccaact
    10921 gatccaacgt cataaatagc gtgcgcattt ctctgtcgag ccgcttcagc aagaaacgcc
    10981 ccacatcttc cggcagttca aaaccacgca aacgcgcgcg taactgtagc gcctgcaact
    11041 tatcttcatc agaaagtggc tgcaatttgt agatctgccc ccagtcgagt cgcgacgcga
    11101 gatccggtaa tcccagattc aactgccgcg gtggacgatc gccggtgatc aacaaccgtg
    11161 ttttgcccga ttccagaatt cgattgtaga gatcgaaaat cgccatctcc cacaactcat
    11221 cgcctgcaat acactcaatg ttgtcgatac aaaccagcga caaatgctcc ataccgtcga
    11281 gcacttccgg aacaaaccaa gtgcgtttat ccagcgggac atagcccacc gcatcgccac
    11341 gctgcgacaa ttccgcgcaa gccgcgtgca gcagatggct gcgccccgcg ccttcgcgtg
    11401 cccagagata gatgtaaccg ctatgttcct gacgcagcac gttttgcagc gcggccagta
    11461 aagaggagtt atcccccggc cagaaacttg caaaggtttc gtcgtcagga agataaagtg
    11521 gcaaagagag ctgtgccggt gtgttcagag atacctcaac caggatttca caaaatcgcg
    11581 agaagtttac cacagaatcc catgatgttt gaaccgggca gcaacactgc ccggtcggta
    11641 cattatttgt ctgttatatc cgcctcttct gcgtccagca ccacttcttc agggcgcagc
    11701 acactgatca gtttgaaaat caaacttaag ccgataccga cgatggtcgc cagcgccata
    11761 ccttttaact ccgccgcgcc gatgtttacc ttcgcgccac tgacgccgat gatcaaaatc
    11821 acggaagtca ggatcaggtt ctgtgctttg ttgtaatcca ctttcgattc gatcaaaaca
    11881 cgaataccgg aagcaccgat gacaccataa agcagcagcg aaacgccgcc catcaccggc
    11941 aatgggatca tctggatagc ggcagccagt ttaccgacgc aggaaagcag gatagcgaaa
    12001 atcgccgccc cgccgataac ccaggtactg taaacacggg tgatcgccat cacgccaatg
    12061 ttttctccgt aagtggtatt tggcgtggag ccaaagaagc cggagatcac ggtcgacagg
    12121 ccgttagcaa acatcgaacg gtgcagacct ggatcgcgca gcagatcttt tttgacgata
    12181 ttagccgtta ctaccaggtg ccctacgtgt tcggcaataa ccactaacgc cgctggcaga
    12241 atagtcagaa tggcaaacca ctcgaagcgc ggcgtataga gggttggcag cgcaaaccag
    12301 tgagcattaa taatcggcgt ggtatcgaca attcccattg cgaaagagag cgcgtagccc
    12361 accagcacgc caattaaaat cgggataatt gccaggaaac cacgaaacag cacggaacct
    12421 aaaaccgtga ccgccagggt ggtaatagag atgatgatgg ttttggagtc tggcgtttgc
    12481 ccttcagccg ggagtaaacc cgccataccg gcagctacgc ccgccagctc cagaccgatg
    12541 acggcaacga ttgcgcccat tgccgcaggt ggaaacagca cgtccagcca gccggtcccc
    12601 gctttcttca cgataaaaga aaccaggcag aacagcacgc cgcacataat aaagccgccc
    12661 agcgcgactt cataccctaa cggcaacagt aacaataccg gtgaaataaa ggcaaagctg
    12721 gaaccaagat aagccggaat tttcccttta cagatgaaga gatacagcag cgttccaata
    12781 ccgttaaata acagtacagt cgccgggtta atatgaaata agacgggcac caggacggtt
    12841 gcaccaaaca tggcgaacaa atgttgcaaa ctaagcggga ttgtctgtaa aagtggcggt
    12901 ctttcactca ccccgatagc acggcgcgtc atagtattat cctctgtatt atgtgttata
    12961 ggcgctttac tcaaaaaaaa gccgactctt aaagtcggct ttaattattt ttattcttta
    13021 tttcgtacca aagattttgt caccggcatc gccgaggccc ggaataatgt atccgtgctc
    13081 gttcagtccc tgatcaatcg atgcggtata cagttcgacg tccgggtgcg ctttttccag
    13141 cgcagcgata ccttctggcg cagcgaccag caccagaact ttgatgctgc tgcagcccgc
    13201 ttttttcagc aggtcgatgg tcgcgataac ggaaccaccg gttgccagca ttgggtcaac
    13261 gatcagcgcc atacgctcat cgatgttaga aaccagtttc tggaagtacg gtaccggctc
    13321 cagcgtttct tcattacggt acataccgac aacgctgatg cgcgcgctcg gaacattttc
    13381 cagcacaccg tccatcatac caagacccgc acgcagaatt ggcacaacgg taattttctt
    13441 acctttgatc tggtcgattt ctaccgggcc gttccagcct tcgatagtta ctttttccgt
    13501 ttcgaggtcg gcggtcgctt cgtaagtcag caggctaccc acttcggaag cgagttcgcg
    13561 aaagcgcttg gtgctgatat cttgctcacg catcagtccc agcttgtgtt tgacgagtgg
    13621 gtgtttgact tccacgatct tcatactctt tctcctttga ggggcagcca caaaaaaatc
    13681 gacggattat acctcctttc ttcaaggcgg caatattctt ttcgttgact ttagtcaaaa
    13741 tgataacgat ttgagataaa gttattttat attcaaatgg ttatgaaaga agatcattcc
    13801 atccgaaaac taacctttgc cctggcacaa gtcttctttc gccgcgcgtc tggggaaaag
    13861 acgtgcaaaa aggttgtgca aagcagcctc gcaaacgttt gctttccctg ttagaattgc
    13921 gccgaatttt atttttctac cgcaagtaac gcgtggggac ccaagcagtg accgataaaa
    13981 cctctcttag ctacaaagat gccggtgttg atattgacgc gggtaatgct ctggttggaa
    14041 gaatcaaagg cgtagtgaag aaaacgcgtc gtccggaagt gatgggcggt ctgggcggct
    14101 tcggtgcgct gtgtgcattg ccgcaaaaat atcgtgaacc cgtgctggtt tccggcactg
    14161 acggcgtagg taccaagctg cgtctggcaa tggacttaaa acgtcacgac accattggta
    14221 ttgatctggt cgccatgtgc gttaatgacc tggtggtgca aggtgcagaa ccgctgtttt
    14281 tcctcgacta ttacgcaacc ggaaaactgg atgttgatac cgcttcagcg gtgatcagcg
    14341 gcatcgcgga aggttgtctg caatcgggct gttcactggt gggtggcgaa acggcagaaa
    14401 tgccggggat gtatcacggt gaagattacg atgtcgcggg tttctgcgtg ggcgtggtag
    14461 aaaaatcaga aatcatcgac ggctctaaag tcagcgacgg cgatgttctg attgcactcg
    14521 gttccagcgg tccgcactcg aacggctatt cgctggtgcg caaaattctt gaagtcagcg
    14581 gttgtgatcc gcaaaccacc gaacttgatg gtaagccatt agccgatcat ctgctggcac
    14641 cgacccgcat ttacgtgaag tcagtgctgg agttgattga aaaggtcgat gtgcatgcca
    14701 ttgcgcacct gaccggcggc ggcttctggg aaaacattcc acgcgtattg ccagataata
    14761 ctcaggcagt gattgatgaa tcttcctggc agtggccgga agtgttcaac tggctgcaaa
    14821 cggcaggtaa cgttgagcgc catgaaatgt atcgcacctt caactgcggc gtcgggatga
    14881 ttattgccct gcctgctccg gaagtggaca aagccctcgc cctgctcaat gccaacggtg
    14941 aaaacgcgtg gaaaatcggt atcatcaaag cctctgattc cgaacaacgc gtggttatcg
    15001 aataatgaat attgtggtgc ttatttccgg taacggaagt aatttacagg caattattga
    15061 cgcctgtaaa accaacaaaa ttaaaggcac cgtacgggca gttttcagca ataaggccga
    15121 cgcgttcggc cttgaacgcg cccgccaggc gggtattgca acgcatacgc tcatcgccag
    15181 cgcgtttgac agtcgtgaag cctatgaccg ggagttgatt catgaaatcg acatgtacgc
    15241 acccgatgtg gtcgtgctgg ctggttttat gcgcattctc agcccggcgt ttgtctccca
    15301 ctatgccggg cgtttgctga acattcaccc ttctctgctg ccgaaatatc ccggattaca
    15361 cacccatcgt caggcgctgg aaaatggcga tgaagagcac ggtacatcgg tgcatttcgt
    15421 caccgatgaa ctggacggtg gcccggttat tttacaggcg aaagtcccgg tatttgctgg
    15481 tgatacggaa gatgacgtca ccgcccgcgt gcaaacccag gaacacgcta tttatccact
    15541 ggtgattagc tggtttgccg atggtcgtct gaaaatacac gaaaacgccg cgtggctgga
    15601 tggtcaacgt ctgccgccgc agggctacgc tgccgacgag taatgccccc gtagttaaag
    15661 cgccagctct gccgctggcg tttttcaatt cacctgtaaa tcgcaagctc cagcagtttt
    15721 tttccccctt ttctggcata gttggacatc tgccaatatt gctcgccata atatccaggc
    15781 agtgtcccgt gaataaaacg gagtaaaagt ggtaatgggt caggaaaagc tatacatcga
    15841 aaaagagctc agttggttat cgttcaatga acgcgtgctt caggaagcgg cggacaaatc
    15901 taacccgctg attgaaagga tgcgtttcct ggggatctat tccaataacc ttgatgagtt
    15961 ctataaagtc cgcttcgctg aactgaagcg acgcatcatt attagcgaag aacaaggctc
    16021 caactctcat tcccgccatt tactgggcaa aattcagtcc cgggtgctga aagccgatca
    16081 ggaattcgac ggcctctaca acgagctatt gctggagatg gcgcgcaacc agatcttcct
    16141 gattaatgaa cgccagctct ccgtcaatca acaaaactgg cttcgtcatt attttaagca
    16201 gtatctgcgt cagcacatta cgccgatttt aatcaatcct gacactgact tagtgcagtt
    16261 cctgaaggat gattacacct atctggcggt ggaaattatc cgtggagaca ccatccgtta
    16321 cgcgctgctg gagatcccat cagataaagt gccgcgcttt gtgaatttac cgccagaagc
    16381 gccgcgtcga cgcaagccga tgattcttct ggataacatt ctgcgttact gccttgatga
    16441 tattttcaaa ggcttctttg attatgacgc gctgaatgcc tattcaatga agatgacccg
    16501 cgatgccgaa tacgatttag tgcatgagat ggaagccagc ctgatggagt tgatgtcttc
    16561 cagtctcaag cagcgtttaa ctgctgagcc ggtgcgtttt gtttatcagc gcgatatgcc
    16621 caatgcgctg gttgaagtgt tacgcgaaaa actgactatt tcccgctacg actccatcgt
    16681 ccccggcggt cgttatcata attttaaaga ctttattaat ttccccaatg tcggcaaagc
    16741 caatctggtg aacaaaccac tgccgcgttt acgccacatt tggtttgata aagcccagtt
    16801 ccgcaatggt tttgatgcca ttcgcgaacg cgatgtgttg ctctattatc cttatcacac
    16861 ctttgagcat gtgctggaac tgctgcgtca ggcttcgttc gacccgagcg tactggcgat
    16921 taaaattaac atttaccgcg tggcgaaaga ttcacgcatc atcgactcga tgatccacgc
    16981 cgcacataac ggtaagaaag tcactgtggt ggttgagtta caggcgcgtt tcgacgaaga
    17041 agccaacatt cactgggcga agcgcctgac cgaagcaggc gtgcacgtta tcttctctgc
    17101 gccggggctg aaaattcacg ccaaactgtt cctgatttca cgtaaagaaa acggtgaagt
    17161 ggtccgttac gcacacatcg ggaccgggaa ctttaacgaa aaaaccgcgc gtctttatac
    17221 tgactattcg ttgctgaccg cagatgcgcg tatcaccaac gaagtacggc gggtatttaa
    17281 ctttattgaa aacccatacc gcccagtgac atttgattat ttaatggtgt caccgcaaaa
    17341 ctctcgccgt ctgttatatg aaatggtaga ccgcgaaatc gccaacgcgc agcaagggct
    17401 gcccagtggt atcaccctga agctaaataa ccttgtcgat aaaggcctgg ttgatcgtct
    17461 gtatgcggcc tccagctccg gcgtaccggt taatctgctg gttcgcggaa tgtgttcgct
    17521 gatccccaat ccggaaggca ttagcgacaa cattcgtgcc atcagtattg ttgaccgtta
    17581 ccttgaacat gaccgggttt atatttttga aaatggcggc gataaaaagg tctacctttc
    17641 ttccgccgac tggatgacgc gcaatattga ttatcgtatt gaagtggcga caccgctgct
    17701 cgatccgcac ctgaagcagc gggtgctgga catcatcgac atattgttca gcgatacggt
    17761 caaagcacgt tatatcgata aagaactcag taatcgctac gttccccgcg gcaatcgccg
    17821 caaagtacgg gcgcagttgg cgatttacga ctacatcaaa tcactcgaac aacctgaata
    17881 accctatgcc aatacacgat aaatcccctc gtccgcagga gtttgctgcg gtcgatcttg
    17941 gttcaaacag ttttcacatg gtcatagccc gtgtggtaga tggtgccatg cagattattg
    18001 gccgcctgaa acagcgagtg catctggcgg acggcctggg gccagataat atgttgagtg
    18061 aagaggcaat gacgcgcggt ttgaactgtc tgtcgctgtt tgccgaacgg ctacaagggt
    18121 tttctcctgc cagcgtctgt atagtcggca cccatacgct gcgtcaggcg ctgaacgcca
    18181 ctgactttct gaaacgcgtg gaaaaggtca ttccctaccc gattgaaatt atttccggta
    18241 atgaagaagc ccgtctgatt tttatgggcg tggaacatac ccaaccggag aaaggtcgca
    18301 aactggttat tgatattggc ggcggatcta cggaactggt gattggtgaa aatttcgaac
    18361 cgattctcgt tgaaagccgc cggatgggtt gtgtcagctt tgcccagctt tacttccctg
    18421 gcggggtcat caataaagag aattttcagc gcgctcgtat ggcggcagca caaaaactgg
    18481 aaactttaac ctggcaattc cgtattcagg gctggaacgt ggcaatgggc gcttccggta
    18541 ccataaaagc cgcccatgaa gtgttaatgg aaatgggcga gaaagacggg ataattaccc
    18601 cggaacgtct ggaaaaactg gtaaaagaag ttttacgtca ccgtaatttc gcatcgctga
    18661 gtttaccggg tctttccgaa gagcggaaaa cagtcttcgt tcctggactg gcgattttat
    18721 gcggtgtgtt tgatgcttta gccatccgtg aactgcgcct ttctgacggg gcgttacgcg
    18781 aaggcgtact gtacgaaatg gaaggacgtt tccgtcatca ggatgtgcgt agtcgcaccg
    18841 ccagcagcct cgccaaccag tatcacatcg acagcgaaca ggcccgacgg gtgctggata
    18901 ccactatgca aatgtacgaa cagtggcggg aacagcaacc gaagctggcg catccgcaac
    18961 tggaggcgct actgcgatgg gccgccatgc tgcatgaggt cgggttgaat atcaaccaca
    19021 gcggtttgca tcgccactcc gcttatattc tgcaaaacag tgacttgccg ggttttaatc
    19081 aggaacagca gctgatgatg gcgacactgg tgcgctatca ccgcaaagcg attaaactcg
    19141 acgatctgcc gcgctttacc ttgtttaaga agaaacagtt cctgccactg attcagctat
    19201 tgcgccttgg cgtattactc aacaatcaac gtcaggcaac caccacaccg ccaacattga
    19261 cactgattac tgatgacagt cactggacac tgcgtttccc gcatgactgg tttagtcaga
    19321 atgcgttggt actgcttgat ctggaaaagg agcaagaata ctgggaaggc gtggctggct
    19381 ggcggttgaa aattgaagaa gaaagtacac ctgaaatcgc cgcttaatgc tttgcgggcc
    19441 gacgagaaat gtcggcccgc attattcagg cactttcgcg aatgggtt
//
LOCUS       DAJTMC010000073        19368 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.073, whole genome shotgun sequence.
ACCESSION   DAJTMC010000073 DAJTMC010000000
VERSION     DAJTMC010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19368
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.073"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(31..1860)
                     /gene="glmS"
                     /locus_tag="OO130_002250"
     CDS             complement(31..1860)
                     /gene="glmS"
                     /locus_tag="OO130_002250"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HCR5365644.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIMVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(2022..3392)
                     /gene="glmU"
                     /locus_tag="OO130_002251"
     CDS             complement(2022..3392)
                     /gene="glmU"
                     /locus_tag="OO130_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HCR5365645.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQESNTGILISNGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDVNLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(3744..4163)
                     /gene="atpC"
                     /locus_tag="OO130_002252"
     CDS             complement(3744..4163)
                     /gene="atpC"
                     /locus_tag="OO130_002252"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HCR5365646.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQSGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(4184..5566)
                     /gene="atpD"
                     /locus_tag="OO130_002253"
     CDS             complement(4184..5566)
                     /gene="atpD"
                     /locus_tag="OO130_002253"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HCR5365647.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(5593..6456)
                     /gene="atpG"
                     /locus_tag="OO130_002254"
     CDS             complement(5593..6456)
                     /gene="atpG"
                     /locus_tag="OO130_002254"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HCR5365648.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHLYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(6507..8048)
                     /gene="atpA"
                     /locus_tag="OO130_002255"
     CDS             complement(6507..8048)
                     /gene="atpA"
                     /locus_tag="OO130_002255"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HCR5365649.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(8061..8594)
                     /gene="atpH"
                     /locus_tag="OO130_002256"
     CDS             complement(8061..8594)
                     /gene="atpH"
                     /locus_tag="OO130_002256"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HCR5365650.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(8609..9079)
                     /gene="atpF"
                     /locus_tag="OO130_002257"
     CDS             complement(8609..9079)
                     /gene="atpF"
                     /locus_tag="OO130_002257"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HCR5365651.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(9141..9380)
                     /gene="atpE"
                     /locus_tag="OO130_002258"
     CDS             complement(9141..9380)
                     /gene="atpE"
                     /locus_tag="OO130_002258"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HCR5365652.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(9427..10242)
                     /gene="atpB"
                     /locus_tag="OO130_002259"
     CDS             complement(9427..10242)
                     /gene="atpB"
                     /locus_tag="OO130_002259"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HCR5365653.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(10251..10631)
                     /gene="atpI"
                     /locus_tag="OO130_002260"
     CDS             complement(10251..10631)
                     /gene="atpI"
                     /locus_tag="OO130_002260"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HCR5365654.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            complement(11248..11871)
                     /gene="rsmG"
                     /locus_tag="OO130_002261"
     CDS             complement(11248..11871)
                     /gene="rsmG"
                     /locus_tag="OO130_002261"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HCR5365655.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            complement(11935..13824)
                     /gene="mnmG"
                     /locus_tag="OO130_002262"
     CDS             complement(11935..13824)
                     /gene="mnmG"
                     /locus_tag="OO130_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HCR5365656.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(14203..14646)
                     /gene="mioC"
                     /locus_tag="OO130_002263"
     CDS             complement(14203..14646)
                     /gene="mioC"
                     /locus_tag="OO130_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HCR5365657.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLIISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            complement(14736..15194)
                     /gene="asnC"
                     /locus_tag="OO130_002264"
     CDS             complement(14736..15194)
                     /gene="asnC"
                     /locus_tag="OO130_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HCR5365658.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQHPIMRTIKP"
     gene            15346..16338
                     /gene="asnA"
                     /locus_tag="OO130_002265"
     CDS             15346..16338
                     /gene="asnA"
                     /locus_tag="OO130_002265"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HCR5365659.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYLDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            complement(16343..17794)
                     /gene="viaA"
                     /locus_tag="OO130_002266"
     CDS             complement(16343..17794)
                     /gene="viaA"
                     /locus_tag="OO130_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HCR5365660.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(17788..19284)
                     /gene="ravA"
                     /locus_tag="OO130_002267"
     CDS             complement(17788..19284)
                     /gene="ravA"
                     /locus_tag="OO130_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HCR5365661.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARALEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSTQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
BASE COUNT         4627 a         5119 c         4851 g         4771 t
ORIGIN      
        1 tcggcagccc ttttttatct atgatagtga ttactcaacc gtaaccgatt ttgccaagtt
       61 acgcggctgg tcaacgtcgg tgcctttgat cagcgcgaca tggtaagcca gcagctgcag
      121 cggaacggtg tagaagatcg gtgcaatcac ctcttccaca tgcggcatct cgatgatgtg
      181 catgttatcg ctacttacaa aacccgcatc ctgatcggcg aagacataca actgaccgcc
      241 acgcgcgcga acttcttcaa tgttggattt cagtttttcc aacagttcgt tgttcggtgc
      301 aacgacgata accggcatat ccgcgtcaat cagcgccagc gggccgtgtt tcagctcgcc
      361 agcagcgtag gcttcagcgt gaatgtagga gatctctttc agcttcaatg cgccttccag
      421 tgcgattggg tactgatcgc cacggcccag gaacagtgcg tgatgtttgt cagagaaatc
      481 ttctgccaga gcttcaatgc gtttgtcctg agatagcatc tgctcaatac ggctcggcag
      541 cgcctgcaga ccatgcacaa tgtcatgttc aatggaggca tccagacctt tcaggcgaga
      601 cagcttcgcc accagcatca acagcacagt taactgagtg gtgaatgctt tagtggacgc
      661 cacgccgatt tccgtacccg cgttggtcat cagcgccaga tcggattcgc gcaccagaga
      721 agaacccgga acgttacaga ttgccagcga accaaggtaa cccagctctt tcgacagacg
      781 caggcccgcc agggtatccg cggtttcgcc agactgtgac aaggtgatca tcaggctgtt
      841 acgacgcacg gcagatttac gatagcggaa ttcagaggcg atttcgacgt cgcacggaat
      901 acctgccagc gattcaaacc agtagcggga aaccataccg gagttataag aagtaccaca
      961 ggcgaggatc tgaatatgct caaccttcga cagcagttcg tcggcgttcg gtcccagctc
     1021 gcttaaatca acctgaccgt ggctgatgcg cccagtaagg gtgtttttga tcgcgttcgg
     1081 ctgttcgtag atctctttct gcatgtagtg acggtaaata cctttatcgc ccgcgtcata
     1141 ttgcagattg gattcgatat cctgacgttt tacttccgcg ccagttttat cgaagatgtt
     1201 taccgaacgg cgagtaattt ccgcaatatc gccctcttca aggaagataa agcgacgggt
     1261 caccggcagc agcgccagct ggtcagaagc gataaagttt tcgcccatac ccaggccaat
     1321 caccagcgga ctaccagaac gtgccgccag cagggtatcc gggtgacggg agtccatgat
     1381 cactgtaccg tacgcaccgc gcagctgcgg gatagcacgc agaacagcct cacgcagagt
     1441 cccgccttgt tttagctccc agttcaccag atgggcaatc acttcggtgt cggtttcaga
     1501 aacgaaggta tagccacgcg cttttaactc ttcacgcagc ggttcatggt tttcgatgat
     1561 gccgttatgc accaccataa tgtgttcaga aacatgcgga tgcgcgttag cttctgaagg
     1621 ttcaccgtgg gtcgcccagc gagtgtgagc aataccggtg ccgccatgca gaggatgttc
     1681 ttccgctgcc tgagccagca tctggacttt accgaggcga cgcaggcggg tcatatgacc
     1741 ttccgcatca acaacggcca gaccagcaga gtcatatccg cggtattcca gacgacgtaa
     1801 accttcaaga aggatttctg ctacatcacg ttgcgcgatc gcgccaacaa ttccacacat
     1861 agtttttgat tccgatttat atcgttgtcg gtcaacctgt atgcccgttt tttcgggcgc
     1921 cccgagcctt gtagagagtg gggttatttt tatagttact gcttgtggga gggagattat
     1981 gttatctcct catcccagat gaccggatta cccggccaga attacttttt ctttaccgga
     2041 cgacgccagc cttctttctg agtctgcggc acacggctga tagctaatgc attttcgccg
     2101 acattacgcg tcacagttgt acccgcagca atggtcgcgc ctttgcctac tgttaccggg
     2161 gccaccagct gagtgtcgga accgacaaac acatcgtcgc cgataatggt cttaaattta
     2221 ttcgcaccat cgtagttgca ggtaatggtt cccgcgccga tgttaacgtt atcgccaatt
     2281 tccgcatcgc ccaggtaagt cagatgacca gctttcgagc ctttacccag acgcgctttt
     2341 ttcatctcaa caaagttacc gacgtgtgca ccttccagca actcagcacc aggacgcaga
     2401 cgggcaaacg ggccaatagt acaggccgct gccagattca cgtcttccac aacggtatac
     2461 ggactgattt cgcaatcatc gccaatcacg ctgtttttaa tcacgcaacc ggtgccgatt
     2521 ttcacgcgat gaccgagagt cacgttgccc tcgatgataa cgttagtatc aatttcaaca
     2581 tcgcgcccgt gagtaagcgt accgcgcaga tcaaaacgcg ccggatcgcg cagcataacg
     2641 cctgctaaca gcagtttttc agcctgttcg gactggtaaa cgcgctccag acgggagagt
     2701 tgcaggcggt tattcacacc ttctacttcg cttaaacgtt gcggatgaac ggcgacgatt
     2761 tcacgccctt cctgatacgc cagcgcaata atgtcggtga tgtagtattc gccctgagca
     2821 ttattgttgg tcagcttcgc cagccagcgt ttcatatctg cgccgttaga gatcagaatg
     2881 ccggtgttgc tctcctgaat ctgacgctgc tcgtcggtgg catctttgtg ctcaacaatg
     2941 ccggtaactt tgccattttc acgggtgata cgtccataac cggtcggatc atccagtttc
     3001 accgtcagca gaccaatgcc accctgcggt ttagcatcac gcaggcgctg gagtgtttcg
     3061 acagagatca gcggcacgtc gccgtagagc attaaaatgt cttcatcatc ggcaaagaaa
     3121 ggtgcggcct gctgcattgc atgacccgta cccagctgct ctgcctgaag cacccagtta
     3181 aggttgtcgt ctttcagcgc ctgtttaagc agatcgccgc cgtgaccgta caccaggtga
     3241 acgtgcgctg cgcctaattc attcgcagca tcaatgacat gctgaaccat cgctttcccg
     3301 gcaagggtat gcagcacttt cggaagatcg gaatacatgc gcgtgccttt gcctgcggca
     3361 aggatcacta cgctcatagc attattcaac atacgcgtcc tgactgtaat ttgagaacga
     3421 atttaaaccg cttcaccttg aaaaaactac atttttttca tcgtgaaatg gacagaggat
     3481 aaattgttca ataacggatt atccccgctg acgaaagtgc cattttcgac catcgtcacg
     3541 ccttttgtct gcagaaaatt aaggcagtac cttcatttaa gcgtcaggtg gatgtttttg
     3601 ctcttatttc gatcaatgaa taaacgggaa aacaggggtg ttttatatca gcaggatcta
     3661 tgtgaacgct cttcaggacg gatcacgcgt aaaaaaaaaa ccagcctgtt tccagactgg
     3721 cttttgtgct tttcaagccg gtgttacatc gcttttttgg tcaactcgat aacgcgcagc
     3781 tgcgcgatcg ctttggccag ttccgcagac gcctgagcgt aatctacgtc gccgtgagag
     3841 ctgctaatgt gctcttcagc cttacgtttc gcttccatgg ctcgcgcttc gtcgagatcc
     3901 tggccgcgaa ttgcggtgtc ggccagaacg gtcacgttgc cagactgcac ttcaagaatg
     3961 ccgccagaca gatagataaa ctcttcgtga ccgtgctgtt tcacgatgcg aatcatacca
     4021 ggcttaatgg cggtgagcag cggtgcgtgg ccagggtaga tccccagttc accttcgcta
     4081 cccgttacct ggattttctc gaccagacca gagaacattt gttgctctgc gctgacgacg
     4141 tccaggtggt aagtcattgc catatcaccc tccgattaag gcgttaaagt tttttggctt
     4201 tttccacagc ttcttcgatg gaaccgacca tgtagaacgc ctgctccggc aggtgatcgt
     4261 attcgccttc catgatgcct ttaaagccac ggatggtgtc tttcagggag acgtatttac
     4321 ccggagaacc ggtgaatact tctgccacga agaacggctg ggacaggaag cgctggatct
     4381 tacgagcacg cgctaccacc agtttgtctt cttcagacag ttcatccata cccaggatgg
     4441 cgatgatgtc tttcagttcc tgataacgtt gcaggatgga ctgaacgcca cgcgcggtgt
     4501 cgtagtgttc ctgaccaacc accagcgggt ccagctgacg gctggtggag tccagcgggt
     4561 caacggccgg gtagataccc agagacgcga tctgacggct cagtaccacg gttgcgtcaa
     4621 ggtgcgcaaa ggtggttgcc ggagacgggt cagtcaagtc atccgcaggt acgtataccg
     4681 cctgtacgga ggtgatagaa ccagttttgg tggaggtgat acgttcctgc agaacgccca
     4741 tctcttccgc cagggtcggc tgataaccta ccgctgaagg catacggccc agcagtgcgg
     4801 atacttccgt accggccagg gtgtaacgat agatgttgtc aacgaacagc agaacgtcac
     4861 gaccttcgtc acggaatttc tcagccatgg tcagaccggt cagagcaacg cgcagacggt
     4921 ttcccggcgg ctcgttcatc tggccataca ccagggatac tttgtcgata acgttggagt
     4981 cggtcatttc gtggtagaag tcgttaccct cacgagtacg ttcacctacg cccgcaaaca
     5041 cagagtaacc ggagtgctcg atcgcgatgt tacgaatgag ctccatcatg tttacggttt
     5101 tacctacacc cgcaccaccg aacagaccaa ctttaccgcc cttagcgaac ggacacatca
     5161 ggtcgataac tttgataccg gtttccagca gttcctgaga gtttgacagc tcttcgtagg
     5221 aaggtgctgc gcggtgaatc gcccaacgct cttcttcacc gatctcgcct ttcatgtcga
     5281 ccggttcacc cagtacgttc atgatacggc ccagagtcgc tttacctacc gggacttcga
     5341 tcgggtgttc gaggtctttt acatccagac cgcgacgcag accgtcggag gaacccattg
     5401 cgatggtacg cacgataccg ccgccgagct gctgctgaac ttccagcacc agacgctcat
     5461 taccattttg cacctcaaga gcatcgtaca cgcgcggtac ggcatcctga gggaattcga
     5521 cgtcaactac ggcgccgatt acctggacaa tctttccagt agccatctta aatcctctac
     5581 gaaataacct gtttaaaccg cggcggcccc cgagacgatc tcggtgagtt cctgagtaat
     5641 gctggcctga cgagctttgt tgtataccaa ctgcagctct ttaatcaggc tgccgccatt
     5701 gtcggtcgcg gctttcatcg ccaccatacg ggcggcctgc tcgctggcca ggttttcaac
     5761 cacgccctga taaacctgag attcgacata acgacgcagc agggtatcca gcaacgcctt
     5821 cggatcgggt tcgtacaggt aatcccagga tttatgtttc agatcatcat catctgatgc
     5881 cggtaacggc agcagctggc tgatggtcgg aacctgagac atggtgttaa taaatttgtt
     5941 gctgacaatg taaagcttgt ccagacggcc ttcgtcgtag gcctgcaaca tcacttttac
     6001 cggaccgatc agttcggaca gggaagggtt atcccccatg ccggtgacct gggcaacaac
     6061 attgccgccc acggagttga agaacgacac gcctttcgag ccgatcattg cgaggtcgca
     6121 ttgaacgcct ttgtcggtcc aggtcttcat ttccgccagc agttttttga acaggttaat
     6181 gttcaaacca ccgcacaaac cacggtcggt cgacaccacc aggtagccca cgcgtttaac
     6241 gtcgcggtct tccaggtaaa ggtgcttata ttccagatta ccatgtgcaa ggtgaccaat
     6301 cactttgcgc atggtttctg cataaggacg gctggccgcc atgcgatcct gcgatttacg
     6361 cattttggaa gcggcgacca tctccatcgc tttagtgatc ttttgcgtgt tctggacgct
     6421 tgcgatctta ctacgtatct cttttgcgcc ggccatgagc ttctcctcaa tgccttgcgg
     6481 cctgccctaa ggcaagccgc cagacgttac caggattggg ttgctttgaa ggaatcgagg
     6541 atgcctttca gtttgccttc gatttcgtcg ttgtagccac cggtctggtt gatctcttgc
     6601 atcaacggag cgtgatcacg gtcgacgtaa gccagcagag cggcttcgaa gctgccaatt
     6661 ttcgacagtt caacatccgc caggtaacca cgttctgctg cgaacagaac cagagactgc
     6721 tgcgcaacgg acatcggcgc atactgtttc tgtttcagca gttcggtcac tttctgaccg
     6781 tggtcaagct gcttacgtgt tgcatcgtca aggtcggatg caaactgaga gaacgctgcc
     6841 agttcacgat actgtgccag agcggtacgg ataccaccgg acagtttttt catgatcttg
     6901 gtctgtgctg caccaccaac acgggatacg gaaatacccg ggttaaccgc aggacgaata
     6961 ccggcgttga acaggttggt ttccaggaag atctgaccat cggtaatgga gattacgttg
     7021 gtcggaacga acgcagaaac gtcacccgcc tgagtttcga taatcggcag tgcggtcaga
     7081 gaaccggttt tccctttcac ttcacctttg gtgaaggctt caacgtattc ggcgttaaca
     7141 cgtgcagcac gctccagcag acgagagtgg aggtagaaaa cgtcgcccgg gaatgcttca
     7201 cgtcctggcg gacgacggag cagcagggag atctgacggt aagcaacagc ctgtttagac
     7261 aggtcatcgt aaatgatcag cgcatcttca ccgcggtcac ggaagtattc gcccattgcg
     7321 caaccggcat acggtgccag gtattgcagt gcagcggatt cagacgcggt tgctaccaca
     7381 acgatggtgt tagccagtgc gccgtgctct tccagtttac gtaccacgtt agaaatggtg
     7441 gacgctttct ggccgatagc gacatagata catttgatac cggaatcgcg ctggttgatg
     7501 atggcatcga tagccagtgc ggttttacct gtctgacggt caccgatgat caattcacgc
     7561 tgaccacgac cgattgggat catggagtca acggctttat aaccggtctg taccggctga
     7621 tctacggact gacgttcgat aacgcccgga gcgattgctt ctacagcaga gaagccgtcg
     7681 tgatccagcg gacctttacc gtcgattggt gcacccagag tgttaaccac acggcccagc
     7741 aggccacggc caaccggaac ttccaggata cggccagtac acttaacttt catgccttcg
     7801 gcaaggtcag cgtacggacc cataacaacc gcacctacag agtcgcgctc gaggttcagt
     7861 gcgatagcgt aacggttacc cggcagggag atcatttcac cctgcataca atcggccagg
     7921 ccgtgaatgc ggataacacc gtcacttaca gaaacaatag taccttcgtt gtgagcttca
     7981 ctcacaacat tgaactgagc aatgcgctgc ttgatcagtt cgctgatttc ggtggaattc
     8041 agttgcatgc tccagtcccc ttaagactgc aagacgtctg caaggcgctc aagacgaccg
     8101 cgtacgctgc catcaatgac catatcaccc gctcggatga taacgcctgc cattacagac
     8161 ttatcgattt tgcaattcag cttaactttg cgtgacagac gtttttccat cgcagcagaa
     8221 attttcgcga gctgttgttc actcagtgcg gcagcggaaa tgacgtctac ctcagcggta
     8281 gcctcactca cggcacgaag gtgaataaac tgctccagaa catccgggag cgcgttaaga
     8341 cgaccatttt cagccattac ccgaatcagg ttctgaccgt tttcgtccag ttgctcacca
     8401 caaactgcga taaacgactc ggcgagcgtt tctggcgcaa gcgcgccaga gagaagctct
     8461 gccatttgtt cgtttttggt tacctcggcg gcaaacgcca gcatgtcctg ccagcgttct
     8521 acactttggt gttcgacggc aaagtcaaaa gctgctttgg cgtaggggcg agctaccgta
     8581 ataaattcag acatcagccc ctccctcctt acagttcagc gacaagttta tccacgatgt
     8641 cgctgttagc agcttcatcc acggaacgtt cgatgatctt ctcggcgcca gcaacagcca
     8701 ggatagcaac ttgcttacgc agctcttcac gggcacgttt acgctcggct tcaatttctg
     8761 cctgcgcctg ggccacgatt ttagtacgtt cctgttctgc ctcagctttc gcttcgtcca
     8821 gaatctgcga gcggcgtttg ttcgcctgct cgatgattac ctgggcttcc gctttcgctt
     8881 ttttcagctg gtcggtcgcg ctggcctttg caaggtcaag gtccttatgt gctcgttctg
     8941 cggaagcaag gccgtcagca atttcttttt gacgtttttc gatggctgcc attaatggcg
     9001 gccatacgta cttcatgcag aacagaacga acaggacaaa cgcgatggcc tggccgagga
     9061 ttgttgcgtt aagattcaca gcacaatgcc tctatttagt taacgttctg atattgctct
     9121 ttaaataaaa gcaacgctta ctacgcgaca gcgaacatca cgtacagacc cagacctaca
     9181 gcgatcatcg ggatagcatc caccagaccc ataacgataa agaactgagt acgcagcaga
     9241 ggaatcagat caggttgacg cgctgcgcct tccaagaatt tacccccgag gatgccgata
     9301 ccgatcgcag caccgattgc cgccagaccc atcatcacag cggcagccat gtacagcaga
     9361 tccatattca ggttttccat gacagtctcc agtttgtttc agttaaaacg tagtagtgtt
     9421 ggtaaattaa tgttcttcag acgccatcga cagatagacg atcgtcagaa ccatgaagat
     9481 gaaggcttgc agcgtaatga tcaggatgtg gaaaatggcc cacggcacat tcaggatcca
     9541 ctgtgaccac cacggcaaca gaccagcaat cagaatgaaa atcagctcac cggcatacat
     9601 gttaccgaac agtcgcaaac cgagtgaaac tggtttggac agcaggctta ccccttcaag
     9661 gattaagttg acaggaatga acgcccagtg attgaacggc tgcagcgtca actctttcgt
     9721 gaagccgccg atgcctttca ttttgatgct gtagaacaga atcaggataa atacgcccaa
     9781 tgccatagac agcgttacgt tcacgtccgc agacggaacc acacgcagtg caggcagacc
     9841 cagtacatgt tcagcaatgt acggcagcag gtcgataggc agtaaatcca tcaggttcat
     9901 caggaatacc cagacgaaga tcgtcagggc cagcggagca atcagcttgc ttttgccatg
     9961 gtacatgtct ttcacgctac cattaacaaa gccgatcacc agctcaatcg cggtctgaaa
    10021 cttacctggc acaccgctgg tcgccttttt ggctacgcta cggaataaaa ccaggaacaa
    10081 cagacccagc accaccgaga agaacatgga gtcaatattg attgtccaga aggtggctgg
    10141 ggggttttgt ggatccacca gcgagaatgt acgcaggtcc agctgaaggt tattcaggtg
    10201 gtgtcctatg taatcctgcg gcgtcatatt ttctgaagcc atgatgcctt ttaccctttg
    10261 ttgttaatta cagccggtgc cagtatctga accaccagca ccaaaaccca cgtaacgatc
    10321 agcggcaaga ataccgcctt taaaaccgcc aacgccacca ccagtaacac caacatcgcc
    10381 agaactttga aagcttcgcc aaatgcgaat gtccaggcca cccggccttt cgctggtgta
    10441 tgcgcctggt gacgccaggc aaatatcata aacaaaacgt taggcagaaa gactgccagg
    10501 cccccgctta ttgcagagac gccccagaag gggtctttga ggctgaacag caatccactt
    10561 gctatcacca ccagtaactg aacgagcaga agcttccgag caacgtttcg actcacgagc
    10621 gacacagaca tcacgttttt tactcctgct cccttcgagg tatgccgcgt gtcgtataaa
    10681 actttcttta aggcttagag tcaagcatca aaaagcggtc aaattatacg gcgcgccccc
    10741 gtgatttcaa acaataagta gccaaaaggt gaataaatgt ttaaatattt ttccagtgca
    10801 tacaattgcg acttttctac taaccctgtt cgatcatgaa aaactgtaaa taacgcgtaa
    10861 acactggtga taaagcgtgc ttcagatcac atattgcgca tgttcgcgca cagcatattt
    10921 atttacttgg caaatgatgc ctttgcaagt ttatgatatt tcagtctaaa aacagatact
    10981 gttttaataa atgacattta cacaacaaaa accacccatt gacattttta ataatgtttt
    11041 agcagccaat gatggttctt agcgccgatt tttagcagac tgatattttc actaatgact
    11101 tattttctgc ttaccaaaaa aagccacgtt atcttgttga tgcaaaagag tgaacgtggc
    11161 gttaaatgta accagttata tcagtagaaa acctggttgt tgttaacagt ctaaccggtc
    11221 aattttttat gatttttttg ataaaaatta aattttattt gctttaatca ccaccagatg
    11281 acgttcgcca tccagggctg gaacctgaag tttaaccact gattcgacct gatattcttc
    11341 gggcaacaaa gcgatttcat cttccggcat ttgccctttc agcgcgtaga aacggccttg
    11401 ctcaccagga agatggtggc accagctcac catatcgttc agagaggcaa aagcgcggct
    11461 aattacgcca tcaaatggcg gctctgaagg aaactcttct accctgctct gtacaggttc
    11521 aatattctcc agtttaagct catgttgcac ctgacgaagg aaacgcacgc gtttaccaag
    11581 gctatccaac agagtgaaat gggcttcagg acgcacgata gagagtggaa tgcctggcag
    11641 tcctggtccg gtgccgacat cgataaaccg ttcaccttgc agatacggtg ccaccacaat
    11701 gctatcgaga atatggcgta ccagcatctc attaggatcg cggaccgaag tcaggttgta
    11761 cgctttgttc catttatgca gcatattcac gtaggcaata agctggtttt tctggtgatc
    11821 ggtaagcgaa atacctgcgt ctttcagcag taaggagagt ttgttgagca cggtgattac
    11881 ctgttcttga tgcgttgcct ggtaagcggg tgcttaccag gcatttttaa tgcgttatgc
    11941 gctacgacgc agcataccct gttttttcag ccacaccagc agaatggaga tagccgcagg
    12001 cgtgacgcca gaaatacgcg aagcttggcc gatagaggct ggtttgtgat cgttaagttt
    12061 ggcgatcact tcgttagaaa gaccggatac ctggcggtaa tccagtgtcg cgggtagcag
    12121 ggtgttctcg ttacgcagct gcttttcgat ctcatcttgc tggcgcgcga tataaccttc
    12181 gtatttaacc tgaatctcaa cctgttccgc cgcctgttcg tctgtcaacg caggggcaaa
    12241 cggcgtcagc gtggttaatt tttcataagt catttccgga cgacgcagca gatcttcacc
    12301 actggcttca cgggaaagcg gcgcagtcag gtgagcattc acttcggctg cagcttccgc
    12361 cgacggggtt acccaggtcg atttcagacg ctgacgctca cgctcgatat tctcaagttt
    12421 ctcgttaaag cgcgcccaac gttcgtcatc caccaggccc agttcacgac cgatttcagt
    12481 caaacgcaga tccgcattat cttcgcgtag catcagacga tattctgcgc gcgaagtaaa
    12541 catacgatac ggttctttgg ttcctaaagt gcacaggtca tcaactagta cgccgagata
    12601 cgcctgagaa cgtgccggag cccaaccttc tttgtcagca gacagacggg cagcgttaag
    12661 accggccagc aaaccttgcg cagcggcttc ttcgtaaccg gtagtgccgt taatctgacc
    12721 agcaaagaac agcccctgga taaacttgct ctccagcgtc ggtttcaggt cgcgaggatc
    12781 gaagaagtca tactcaatgg cataacccgg acgcacgatc ttcgcgtttt ccatcccctg
    12841 catagagcgg acgatttgca tctgcacatc gaacggcagg ctggtggaga taccgttcgg
    12901 ataaatttca ttagaggtca gtccttccgg ttcaaggaag atctgatgct gatttctgtc
    12961 ggcgaagcgc atgactttgt cttcgatcga cgggcagtag cgtgggccga caccttcgat
    13021 cacccctgca tacattgggc tacgatcgag gttactgcgg atcacatcat gggttttctc
    13081 gttggtatga gtgatataac acggcacctg ctggggatgc tgggacgcat tgcccataaa
    13141 cgagaatacc ggcattgggt tatcgccatg ctgttgcgcc agcacactaa agtcgatagt
    13201 tcgagcatca atacgcggtg gtgtcccggt tttcagacga ccaacgcgca gcggcagttc
    13261 acgcaaacgg cgagaaagcg gaatggacgg cggatcacca gcacggccac cgctgtaatt
    13321 atccagaccg atatgaattt taccgtcgag gaacgtccca acggtgagca cgactgcttt
    13381 ggcacggaac ttcagtccca tttgggtaac agcaccgacc acgcgatcgt tttcgacaat
    13441 aagatcttca accgcctgct ggaagatcat caggttcggt tggttctcca gcgccgtacg
    13501 taccgcctga cggtagagca cacgatccgc ctgagctcgg gtagcgcgaa ccgccggtcc
    13561 tttgcttgcg tttagtatcc taaactggat acccgcttga tcgatcgctt tcgccatcag
    13621 accgccgagt gcatccactt cttttaccag atgtcccttc ccaataccgc cgatcgccgg
    13681 gttgcagctc atctgcccca gagtgtcgat attgtgtgtc aaaagcagag tctgttgacc
    13741 catacgcgcc gcggccatcg cggcctcggt gcctgcatga cccccgccaa tgatgatgac
    13801 gtcaaaagga tccggataaa acatggtgat tgcctcgcat aacgcggtat gaaaatggat
    13861 tgaagcccgg gccgtggatt ctactcaact ttgtcggctt gagaaagacc tgggatcctg
    13921 ggtattaaaa aggagatcta tttatttaga gatctgttct attgtgatct cttattagga
    13981 tcgcactgcc ctgtggataa caaggatccg gcttttaaga tcaacaacct ggaaaggatc
    14041 attaactgtg aatgatcggt gatcctggac cgtataagct gggatcagaa tgaggggtta
    14101 tacacaactc aaaaactgaa caaccgttgt tccttggata actaccggtt gaaccaagct
    14161 tcctgacaga gttatccaca gtagaacgca cgatctgtat acttatttga gtaaattaat
    14221 ccacgatccc agccattctt ctgccggatc ttccggaatg tcgtgatcaa gaatgttgat
    14281 cttcagtgtt tcgcctgtct gttttgcacc ggaatttttg agttctgcct cgagtttatc
    14341 gatagcccca caaaaggtgt catattcacg actaccaata ccgattgcgc caaagcggac
    14401 cgcagaaaga tcgggcttct gttcctgcaa tgcttcatag aaaggagaaa ggttgtccgg
    14461 aatatctccg gcaccgtggg tggagctgat aatcagccag atccccgagg cagataaatc
    14521 ttccaacagc ggaccgtgca gcgtttcggt ggtaaaaccc gcctcttcca gcttttcagc
    14581 caggtgttct gctacatatt cggcaccgcc gagggtgctg ccgctgataa gagtgatatc
    14641 tgccataaac cgccaccttt attaagagtg gcgtattgta cgctgtgaac gcgttgggat
    14701 ctacctgtgg aaaagtatgg gattaaaaaa gccgatcagg gcttgatagt acgcatgatc
    14761 gggtgttgca ggacgatcaa tgtctcggtg gactgaattt catcaattgt ttggatcttg
    14821 ttgataagta catgctggag agcgtcgatc gaacggcaca ttacttttat aaagatgctg
    14881 tagtggcctg ttgtgtaata ggcttcagtg acttcatcaa ggctttccag ctttgccagc
    14941 gcggaagggt agtctttggc gctctttaat ataatgccga taaagcagcc tacgtcataa
    15001 ccgagctgct tcgggctgac atcaatacgc gccccggtaa tgatccccgc ctgcttcatt
    15061 ttctctaccc gaacgtgaat cgtccccgga ctgacgccaa attgtttcgc cagttcggcg
    15121 taagcggtgc gcgcattgcc cattaatgct tccaggatgc cacggtccag attgtcgatc
    15181 agataatttt ccataggatt ttcttatgca gattgatgat tcattctatt ttagccttct
    15241 tttttaatga atcaaaagtg tgttaggctt tttattgaat gattattgca tgtgtgtcgg
    15301 tttttgttgc ttaatcataa gcaacaggac gcaggagtat aaaaaatgaa aaccgcttac
    15361 attgccaaac aacgccaaat tagcttcgtg aaatctcact tttctcgtca actggaagaa
    15421 cgtctggggc tgatcgaagt ccaggcaccg attcttagcc gtgtggggga tggcacgcag
    15481 gataacttgt cgggctgtga aaaagcggtg caggtaaaag tgaaagctct gcctgatgcc
    15541 cagttcgaag tggttcattc actggcgaag tggaaacgtc agaccttagg gcaacacgac
    15601 ttcagcgcgg gcgaagggct gtacacgcac atgaaagccc ttcgccccga tgaagaccgt
    15661 ctttctccgt tgcactcggt ctatgtagac cagtgggact gggaacgcgt aatgggcgac
    15721 ggtgagcgtc aattctcgac tctgaaaagc acggtagagg cgatctgggc gggaattaaa
    15781 gcaaccgaag ctgcggttag cgaagagttt ggcctggcac cgttcctgcc ggatcagatc
    15841 cacttcgtac acagccagga gttactgtct cgttatctgg atctcgatgc caaagggcgt
    15901 gagcgggcga tagcgaaaga tcttggcgcg gtattccttg tcgggattgg cggcaagctg
    15961 agcgatggtc atcgccacga cgtgcgcgca ccggattatg atgactggag caccccgtca
    16021 gagctgggtc atgcgggtct gaacggcgat attctggtgt ggaaccctgt actggaagat
    16081 gcgtttgagc tttcttccat ggggatccgc gtggatgccg acacgctgaa gcatcagctg
    16141 gcgctgaccg gtgatgaaga tcgtctgcag ctggaatggc atcaggcgct gctgcgcggt
    16201 gaaatgccgc agaccatcgg cggcggtatc ggccagtctc gtttgactat gctgctgctg
    16261 caactgccgc atatcggcca ggttcagtgt ggagtatggc cagctgctgt tcgcgagagc
    16321 gtcccttctc tgctgtaata atttatcgcc gccagcgtct gagcaggcgg cttcgcatcc
    16381 cggtatcaaa gcgccagata tgatcgaaaa tgcgcatgat gccgggtttg ccgtgtgccg
    16441 acatcgccac ggcatgaaag cgatgctgat gtacccgctg cagctctttc actttactcg
    16501 tcacgtcgtc aggcaaccgc tgagcgataa aatcagaaat caccaccgca tcggcatcaa
    16561 accattccct gctttgcaag cgttccataa tggcgcgaaa acaactggca agatcggtgc
    16621 cgccacgaaa ctgctggctt aaaaaacgga ttgcttgttc gatgccttgt gggcctgaaa
    16681 gctcataacg gacgatctcg gtggaaaata gcataatata gcagcgccgg ttttctgcga
    16741 gagcaatgcg catcaaggcc aggcagaacg ctttcgcaca ctgttcatta aagccgccca
    16801 ttgagccgga agtatccaca cagacaataa acggcccgcg cggctgttca tcgtaatctt
    16861 tatgtaccac cggacgttcg atcacttttt cacgccacga ctcaccgtgc aggcgatagg
    16921 tgagcaactg tttttccacc agccgacggt aaaactcata ctccagttcc gttatcccta
    16981 gtgtcgccag ttctggcggc aggagacgta aaatatcatc gctttgttgc agaccatcaa
    17041 cctgctcagg aaccgtcgcc ggttcgcgca ccatggtgcg gaaggtttcc atctgcgcat
    17101 cgttgcgcgg tattgatttg gcttcccgag aacgccccag ctgctctgcc aggcgtttca
    17161 gttccggctg ttcgttaaga aattcaccgt atttcacaat caactgatag tcgccacgtt
    17221 taagctggcc ggcgctcata tcccacagac gaccagctgc ggtattgtta tctgcgagaa
    17281 tcggttcaag ttgtccgctc agcgtcatgc gttcctgaac ttcacttaac agttgttcgc
    17341 gttcttcttc taatagctgt tgatttaacg tcgttgcttg cacgatcaga cttaaacgcc
    17401 agcgctggag aaaaagcgtg tgtaacgctg aagttattgt gctgttagca tcaaccaact
    17461 gccgggcttg ttctgcccat ggggaattca gacgatgcag taagtccagg atctgtggta
    17521 gctgcacaat aaactgcggc gtggagagga gctggctttg ctgatagcac atcacctctt
    17581 cggtgagttc tggcgggact cgggcatctt tcagccgact gcgcagcgcc tcacgccagc
    17641 ggggaacatc atcagtgatt gctgccttca gtcgtgggaa tttttcaaag aagaccgcca
    17701 gctgcggtga ggccagcagt gcgatgatca tctcttcgat caatccctct tcgctgacgg
    17761 ccagcatcac attaagcgta tccagcgtta gcattgttgt gcctggcgaa tctgtgcacc
    17821 gacatcctgt aggctggctt caatgcgacc taaccagtcg ccagggataa acagacattt
    17881 ttgctgttcg ctgaataaag cgtgttgctt acgccagtcg ctttcaagct cttccagttg
    17941 ttgttttatt tcacctggca gaccttcagc cgatgaaccg ggaagtgcca gcgtactgcc
    18001 ttgtaaactt acatcgcgta caacaagatg ttgggtgcta tcaacttcca gattcagttt
    18061 ctgggcaaag ccgataccgt tcagtttgcc gcgaatttca ccacctttac tcagccactg
    18121 ttccagcgcg ctacgctcaa aggagatatg aaccacttcc atatcatgca gttttaacgg
    18181 tttttgcagc agcagagtca gagtggaagc agtaacgtta acagggagtt gatactgctg
    18241 acgacggcta aaaatgccgc ccagacgaat taccgttaag gctgttttat cgctttgttg
    18301 ctgctgaagt tgcaggtgac gttgcacaat cgcgcctagg cgggtcaaca tcccttgctg
    18361 ttgccaggcg tgaccggtca tcaatacatc aatttgttgt tgtatcaaat tcaggctttg
    18421 cgcgtcatac cacaggcaat ctttcagcaa aatgagatct accggggcaa cagcactgcg
    18481 accgctaaaa aaggcgctgg cctgcaataa tcggatcgct tttttccagc gacgatccga
    18541 gacataaggc gcatccggta atttatccag ttgctggcgc agcataaaaa tcagctcaaa
    18601 tacatgatcg ggcagcgtaa tttcaccaat ctctttctgc cagcgttcat attcttcatc
    18661 tgtgacctgc aaggcatcag gaaccggatt gtcgttttca tcctgttgac tggtcagcat
    18721 ggagcggaaa ttcgctttat cctgcacttt atctaaccac agacgaatca gcatgcggtc
    18781 atataacgct tccagactgc tgtctgcttc cggcagctcg ttggaggccg ccaccagcag
    18841 acgcatcggg attttttcta cgtgtgcacc gttgcggaac tggcgctcgt taatggcggt
    18901 gagcaaggta ttaagaattg ccgggcccgc tttccagatc tcatccagaa agacaatttc
    18961 tgcttccggc aggtaaccgc tggttaaacg ttcatagcgc ccttcatctt ttagcgcctg
    19021 aatagaaagg ggaccaaaaa cttcttccgg cgtggagaag cgggtcatca gatattcaag
    19081 cgcgcgggca ttctgaaagg cgaattttaa gcgccgggcg atcaaacttt tggcaatacc
    19141 tggcgggcca aggaggaaca cactttcacc acttaatgcc gctaataaac acaagcggat
    19201 ggcgtggcta cgttcataaa gccccttttc caacgaactg ctcaggcggg aaattctttc
    19261 cgctaataaa tgagggtgag ccataatgaa gtggcgtcct ttcgtcaaaa gttctgcgta
    19321 aattgcgagt atagacgttt cttgctggtg gctaaaatag tctcaaag
//
LOCUS       DAJTMC010000074        19312 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.074, whole genome shotgun sequence.
ACCESSION   DAJTMC010000074 DAJTMC010000000
VERSION     DAJTMC010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19312
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.074"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            <1..731
                     /gene="rihB"
                     /locus_tag="OO130_002268"
     CDS             <1..731
                     /gene="rihB"
                     /locus_tag="OO130_002268"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HCR5365662.1"
                     /translation="MRKQIVADNIHGETGLDGPVFESLTRQAESTHAVKYIIDTLMAS
                     DGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFTPSAEFNIFADPEA
                     ARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGELFSDIMNFTLKTQFENY
                     GLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCDELGVLGKPANTKV
                     GITIDTDWFWGLVEECVRGYIKTH"
     gene            831..2081
                     /locus_tag="OO130_002269"
     CDS             831..2081
                     /locus_tag="OO130_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCR5365663.1"
                     /translation="MDVMRSVLGMVVLLTIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPAGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDHLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEAAATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFAHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTGGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(2137..3225)
                     /locus_tag="OO130_002270"
     CDS             complement(2137..3225)
                     /locus_tag="OO130_002270"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCR5365664.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKGKGYILTEQEYCVVVGTINMDIRGIADIRYPQAASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDQT
                     VLAINDTHLLEQLTPQLLNGSRDLLRHAGVVLADCNLTAEALEWVFTLADEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTAPAHTTVDSFGAGDGFMAGLVYSFLEGYSFRDSARFAVA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(3228..4085)
                     /gene="nfo"
                     /locus_tag="OO130_002271"
     CDS             complement(3228..4085)
                     /gene="nfo"
                     /locus_tag="OO130_002271"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HCR5365665.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(4159..5208)
                     /locus_tag="OO130_002272"
     CDS             complement(4159..5208)
                     /locus_tag="OO130_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HCR5365666.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIIVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            5308..6189
                     /locus_tag="OO130_002273"
     CDS             5308..6189
                     /locus_tag="OO130_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5365667.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANIPR"
     gene            6394..7863
                     /gene="lysP"
                     /locus_tag="OO130_002274"
     CDS             6394..7863
                     /gene="lysP"
                     /locus_tag="OO130_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HCR5365668.1"
                     /translation="MVSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            8156..10136
                     /gene="cirA"
                     /locus_tag="OO130_002275"
                     /pseudo
     CDS             8156..10136
                     /gene="cirA"
                     /locus_tag="OO130_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
     gene            complement(10168..11004)
                     /gene="yeiG"
                     /locus_tag="OO130_002276"
     CDS             complement(10168..11004)
                     /gene="yeiG"
                     /locus_tag="OO130_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HCR5365669.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEKVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSSYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            11262..11930
                     /gene="folE"
                     /locus_tag="OO130_002277"
     CDS             11262..11930
                     /gene="folE"
                     /locus_tag="OO130_002277"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HCR5365670.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            11947..13104
                     /locus_tag="OO130_002278"
     CDS             11947..13104
                     /locus_tag="OO130_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 family protein"
                     /protein_id="HCR5365671.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRA
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTMLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGTAISKTSR"
     gene            13246..14286
                     /gene="galS"
                     /locus_tag="OO130_002279"
     CDS             13246..14286
                     /gene="galS"
                     /locus_tag="OO130_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HCR5365672.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAIFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            14566..15564
                     /gene="mglB"
                     /locus_tag="OO130_002280"
     CDS             14566..15564
                     /gene="mglB"
                     /locus_tag="OO130_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HCR5365673.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQSVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            15625..17145
                     /gene="mglA"
                     /locus_tag="OO130_002281"
     CDS             15625..17145
                     /gene="mglA"
                     /locus_tag="OO130_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HCR5365674.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDKSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            17161..18171
                     /gene="mglC"
                     /locus_tag="OO130_002282"
     CDS             17161..18171
                     /gene="mglC"
                     /locus_tag="OO130_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HCR5365675.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPCWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(18414..19312)
                     /gene="preA"
                     /locus_tag="OO130_002283"
                     /pseudo
     CDS             complement(18414..19312)
                     /gene="preA"
                     /locus_tag="OO130_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
BASE COUNT         4601 a         4774 c         5120 g         4817 t
ORIGIN      
        1 ttatgcgtaa acaaatcgtt gccgataata ttcacggtga aaccggactg gatggtccgg
       61 tattcgagtc gctgacccgc caggcagaaa gtacccacgc ggtgaaatac atcattgata
      121 ccctgatggc aagcgatggc gatatcactc tggtgccggt tggtccgctt tcaaatatcg
      181 cggtggcaat gcgtatgcaa cccgcgatcc tgcccaaaat ccgtgaaatt gtgctaatgg
      241 gcggcgctta cggtacaggc aacttcacgc catctgccga gttcaacatc tttgctgatc
      301 cggaagcagc ccgcgtggtg ttcacctccg gcgttccatt agtgatgatg ggcctcgatc
      361 tcaccaacca gactgtttgc accccggacg tgattgcacg gatggaaagg gcaggcggcc
      421 ctgccggaga gctgttcagc gacatcatga acttcactct caaaacgcag ttcgaaaact
      481 acggcctggc tggcggcccg gtgcacgacg ccacctgcat cggttatctg attaaccctg
      541 atggcattaa aacccaggag atgtacgtcg aagtagacgt caacagtggc ccttgctatg
      601 ggcgtaccgt ctgcgacgag ctgggcgttc ttggcaagcc cgctaatacc aaagtcggca
      661 tcactattga taccgactgg ttctggggct tagtcgaaga gtgcgtgcgc ggctacatca
      721 aaacccatta agtgttgata acaagccggg tcagtatcgc aagatccaag cccggctatt
      781 tctgatgggc agaaagccgt cgcccaatat gacatgaaga gataataact atggatgtca
      841 tgagaagtgt tctgggaatg gtggtattgc tgacgattgc gtttttgctg tcagtaaaca
      901 agaagaagat cagcctgcgt accgttggcg cggcgttagt gttacaggtc gtgattggcg
      961 ggattatgct ttggttaccg gcagggcgtt gggtcgctga aaaagtcgct tttggcgtgc
     1021 ataaagtgat ggcgtacagc gacgcgggta gcgcatttat cttcggttcg ctggtcgggc
     1081 cgaaaatgga cacgctgttt gatggcgcag gatttatctt tggtttcagg gtattaccgg
     1141 caattatctt cgtcactgca ctggtgagta ttctctacta catcggtgtg atggggattt
     1201 taattcgcat tctcggcggt atattccaga aagcattaaa tatcagcaag attgagtcat
     1261 tcgtcgcggt caccaccatt ttcctcgggc aaaacgaaat tccggcgatc gtcaaaccct
     1321 ttatcgatca tctgaatcgc aatgaattat ttacagcgat ttgtagtggc atggcctcga
     1381 ttgctggttc gacaatgatt ggttacgccg ccctgggcgt acctgtggaa tatttgctgg
     1441 cggcatcgtt aatggcgatc ccaggcggga tcttgtttgc tcgcctgtta agcccggcta
     1501 cggaatcttc gcaggtttcc tttaataacc tctctttcac cgaaacaccg ccaaaaagca
     1561 ttattgaagc cgctgcgaca ggggcaatga ccgggctgaa aatcgccgcc ggtgtagcga
     1621 cagttgttat ggcatttgtc gccatcattg cgttaattaa tggtattatc ggcggcgttg
     1681 gcggctggtt tggttttgca catgcctcgc tggagtccat tttaggttac ctgttggccc
     1741 cattggcgtg ggtgatgggg gttgactgga gtgatgcaaa tcttgccggg agtttgattg
     1801 ggcagaagct ggcgatcaat gaatttgtcg cttatctcaa tttctcgcca tatctgcaaa
     1861 cgggtggcac tctggatgct aaaaccgtgg cgattatttc tttcgcgttg tgcggtttcg
     1921 ctaactttgg ttctatcggg gtggtggtgg gggcgttttc tgcggttgcg ccacaccgtg
     1981 cgccggaaat cgcccaactt ggtttacgcg cgctggcggc ggcgacactt tctaacctga
     2041 tgagtgctac tattgcagga ttctttattg gtttagcgta gtgcgcaatt tatgagccgg
     2101 ataagatgcg gtcaagcgtc gaatccggca acattgttac accattggca ctaatgaaag
     2161 cgcgttatcg gcagacaggg tagggttgtt taagctgccg ctggcgcgcg aaattgccgc
     2221 gcaggctacc gcaaaacggg cgctgtcgcg gaaactgtat ccttcaagaa agctatatac
     2281 caggcccgcc ataaaaccat cgccagcacc aaaactgtct actgtcgtat gcgctggcgc
     2341 ggtcagcaaa aattgttctc catccttttc gctgcaataa actgactcat cgggcaaata
     2401 aacaaacagt tgctgaacac cttgctgatg caatgcattc actgcggcat tacggtcagc
     2461 atcgctggtg atcgcctgtc cccataaaat ttccagctcc ggtaaagtgg gtttcagggt
     2521 gtgaatatgc gccagccagt gtttgatttt gcccgctttg aattctgaaa cggtatcgac
     2581 aaacaccggg atttcatcag cgagggtaaa gacccattcc agcgcctcgg ctgtcaggtt
     2641 gcaatcagcc agtaccacgc ccgcatgacg aagtaaatcg cgcgacccgt tcagtagttg
     2701 cggtgtcaac tgttccagca gatgggtatc gttaatcgcc agcacggttt gatcgtctcg
     2761 attggcaatt gccagatacg tcgatgtgct ttgaccatgc aaacgaacgc agccggagac
     2821 attcacgccg gcacggcgcg tttcttccag gagcatttcg ccataaaagt catcgccaat
     2881 cactgaaagc aaatggacgt cacggcctaa cagcgccaga ttgtgggcga tgttgcgtcc
     2941 cacgccgccc gccgagcaat gaattgtacc gggatgagaa gccgcttgcg ggtaacggat
     3001 atccgcgatc ccgcgaatat ccatattgat tgtccccacc actacgcagt attcctgctc
     3061 ggtgagaatg taacctttgc ctttaatccg ccctttgcgc attaaatcca taatatgcgc
     3121 cgcaacgcgc gaacgactga tttgcagcat gtccgcaatt tcgttctgct gaatcagcgg
     3181 gttacgccgt aaaattgcaa ggatctcctt ttcccggtta ttcatcttca ggctaccgct
     3241 ttttcagttt gttgcgcttt cagccaggcg atctcttctg cccagatatc cgggttgatg
     3301 gtttcaagga tcagcggaat gccgtcgaaa cggtcgtcct gcatgatcca gcggaacgca
     3361 tcatgaccga tattgccttc accgaggcta tgatggcggt caacgcggct gccaaaggtg
     3421 cttttcgcat cgttaaggtg catcccgcgc agatacttaa agccaacaat acgggcaaaa
     3481 tccgcgaacg ttttctcgca ttcggctgga gtacgcaaat catacccggc agcgaaagca
     3541 tggcaggtat caatgcagac gccgacacgg gatttatctt ccacgccgtc gataatcgcc
     3601 gcgagatgtt cgaatttaaa ccctaagtta ctgccctgac cggcggtgtt ttcaatcacc
     3661 gctgttacac cttgagtttt atccagcgca atgttgatgg attcggcaat acgcgcaagg
     3721 caatcctctt ctgaaatctg catcagatgg ctgccagggt ggaagttgag caaagaaagc
     3781 cccagttgtt cgcaacgctg catttcatct ataaaggcat cgcgcgattt ttccagagct
     3841 tcagtgaccg gatgtccgag gttaatcaga tagctgtcgt ggggaagaat ttgcgccgat
     3901 gtgtagtgat atttttcaca ggctgctttg aattcatcga tggtttgcgt cgtgagcggt
     3961 gcggcacgcc actgacgttg gtttttggtg aacaaggcaa acgcggttgc gtcgatttcg
     4021 gcggcgcgaa ttgcggcatt tgccagaccg ccagcagcac taacgtgcgc tccaatgtat
     4081 ttcatgcgag gactcctgtt aaacccgctg gaggaaaacg gtaatgatag cgggttaaca
     4141 ggagcaagat gtagtggttt atgcgatgac gctttgaatc acatagttaa tcgcaccacc
     4201 accaacaatc agccaggcaa acagtaccag tgccatcagc agaggtttcg ccccggcttt
     4261 tttcagcgcg ctgacgtgag tggtcagacc cagcgccgcc atcgccattg ccagcaggaa
     4321 ggtatccagc gttaccagca tgttcaccac gctctgcggt aacaggtgga acgagttaaa
     4381 gatggcaacg atgatgaaca agatggcaaa ccacggaata gtgattttgc ttttctcgcc
     4441 gctgttcgcc ccagacagct gtttaacacg cgccgccagc aggatgagga acggagccag
     4501 catcatcacg cgcagcattt tggaaataac tgctgcgttt tccgcatccg ggctgatggc
     4561 atgacctgcc gccaccacct gcgccacttc gtgcacagta gaaccaatgt agataccgaa
     4621 agtttccgga ctaaaccatt gagacatcag cggatatatc gccgggtaga ggaaaatcgc
     4681 gacggtcccg aagataacaa cggttgcaac agccacggtt actttactgg cttccgcttt
     4741 cactaccggc tcagtcgcca gtaccgcggc agcaccacag atactgctac cggcaccgat
     4801 caaccagctg gtgtgcttat ccagaccaaa cactttctgc cccaggaagc aagccagcag
     4861 gaaggtactg gacagcgtca acacgtcaat gatgatccca ctgataccga catcggcaat
     4921 ttgcgagaac gtcagacgga agccataaag aatgataccc agacgtaata aatattgctt
     4981 ggcaaacagc acaccaccgt cacagctttt ccagatgtgc ggatagatgg tgttgcctaa
     5041 aaccatcccc aacaagattg cgagggtgag ggcactaaac ccggcacccg caaccgcggg
     5101 aatggaacca ccccacaggg cgaccccggt gataactgca ctcagggcta accccggaat
     5161 aaaatgccac agtgtacgat gttgtttctg taaggtgata ttcgtcataa ccctctcctt
     5221 ataaccgggc ataaggttac gactaactgg tttaaaaata aaattgatta tatatttata
     5281 attaatcttt ataagtggta agcgactatg cacatcaccc tccggcagtt ggaagttttt
     5341 gcagaagtat tgaaaagtgg atcaaccacc caggcgtcgg tgatgctggc gttgtcgcaa
     5401 tcagcagtga gcgcagcctt gaccgacctg gaagggcagc ttggcgtgca actgtttgat
     5461 cgcgtgggga aaagactggt tgttaatgaa cacgggcggc tgctctatcc gcgtgcgttg
     5521 gcattgcttg aacaggcggt tgaaatcgaa caactgtttc gcgaagacaa cggcgcgatt
     5581 cgtatctatg ccagtagtac catcggtaac tacattctgc ctgcagttat cgcccgttat
     5641 cgccatgatt atccgcagtt gccgattgaa cttagcgttg ggaacagcca ggacgtgatg
     5701 caagcggtgc tggatttccg cgttgatatt ggctttattg aaggaccgtg ccacagcact
     5761 gaaatcattt ctgaaccgtg gctggaagac gagctggtgg ttttcgccgc gccgacttcg
     5821 ccgttggctc gtggtccggt caccttagaa cagctggccg ctgcgccgtg gatcctgcgt
     5881 gaacgcggtt ccggcacgcg ggagattgtc gattatctat tgctgtcaca tttaccgaag
     5941 tttgagatgg cgatggaatt aggtaactcc gaggcaatca aacatgcggt gcgtcatggg
     6001 ttgggaatta gttgcctgtc gcgacgggtg attgaagatc aattgcaggc aggcacatta
     6061 agtgaagttg cggtccctct gccgcgcctg atgcgtacgt tgtggcgtat acatcatcgg
     6121 caaaaacacc tttccaacgc gctacggcgc tttctggact attgcgatcc cgcgaatata
     6181 ccgcgttaag ttgctgtaca agaacatgct ggtgctgtgt cgatttcgtg acgcagcgcc
     6241 ctcagcatgc attcgccaga aaagagattg gctgctttac ttataatccc agggcgatca
     6301 tgaaggtgtc ttataaccgt gtatttctgc cggaaggatt gccaatcgtc tgctacaatc
     6361 gcgcctcatt tttaagatgg atagcatttt tgtatggttt ccgaaactaa aaccacagaa
     6421 gcgccgggct tacgccgtga attaaaggcg cgtcacctga cgatgattgc cattggcggt
     6481 tccatcggta caggtctttt tgttgcctct ggcgcaacga tttctcaggc aggtccgggc
     6541 ggggcattgc tctcgtatat gctgattggc ctgatggttt acttcctgat gaccagtctc
     6601 ggtgaactgg ctgcatatat gccggtttcc ggttcgtttg ccacttacgg tcagaactat
     6661 gttgaagaag gctttggctt cgcgctgggc tggaactact ggtacaactg ggcggtgact
     6721 atcgccgttg acctggttgc agctcagctg gtcatgagct ggtggttccc ggatacaccg
     6781 ggctggatct ggagtgcgtt gttcctcggc gttatcttcc tgctgaacta catctcagtt
     6841 cgtggctttg gtgaagcgga atactggttc tcactgatca aagtcacgac agttattgtc
     6901 tttatcatcg ttggcgtgct gatgattatc ggtatcttca aaggcgcgca gcctgcgggc
     6961 tggagcaact ggacaatcgg cgaagcgccg tttgctggtg gttttgcggc gatgatcggc
     7021 gtagctatga ttgtcggctt ctctttccag ggaaccgagc tgatcggtat tgctgcaggc
     7081 gagtccgaag atccggcgaa aaacattcca cgcgcggtac gtcaggtgtt ctggcgaatc
     7141 ctgttgttct atgtgttcgc gatcctgatt atcagcctga ttattccgta caccgatccg
     7201 agcctgctgc gtaacgatgt taaagacatc agcgttagtc cgttcaccct ggtgttccag
     7261 cacgcgggtc tgctctctgc ggcggcggtg atgaacgcag ttattctgac ggcggtgctg
     7321 tcagccggta actccggtat gtatgcgtct actcgtatgc tgtacaccct ggcgtgtgac
     7381 ggtaaagcgc cgcgcatttt cgctaaactg tcgcgtggtg gcgtgccgcg taatgcgctg
     7441 tatgcgacga cggtgattgc cggtctgtgc ttcctgacct ccatgtttgg caaccagacg
     7501 gtatacctgt ggctgctgaa cacctccggg atgacgggtt ttatcgcctg gctggggatt
     7561 gccattagcc actatcgctt ccgtcgcggt tacgtattgc agggacacga cattaacgat
     7621 ctgccgtacc gttcaggttt cttcccactg gggccgatct tcgcattcat tctgtgtctg
     7681 attatcactt tgggccagaa ctacgaagcg ttcctgaaag ataccattga ctggggcggc
     7741 gtagcggcaa cgtatattgg tatcccgctg ttcctgatta tttggttcgg ctacaagctg
     7801 attaaaggaa ctcacttcgt acgctacagc gaaatgaagt tcccgcagaa cgataagaaa
     7861 taagtttcct cccttccttg ctaagccctc tcaaccgaga gggctttttc aattccattt
     7921 ccctgacaaa tcatgcggat ataaaattta acatttggat tgataattgt tatcgtttgc
     7981 attatcgtta cgccgcaatc aaaaaaggct gacaaatcag aggctgttcc ggctttctgg
     8041 gatgatcacc tgcatacaaa taagtccacc gcgatgctgc cgtacgcaag gggacgtgaa
     8101 gaaggtgtga gcgataaccc attttatttt cgtagttacc tcattgagat atggaatgtt
     8161 taggttgaac cctttcgtac gggtcaggct gtgtttgtcc gctatttctt gtgcatggcc
     8221 tgtgttagcg gtcgatgatg atggcgaaac gatggttgtc actgcatctt ccgttgaaca
     8281 aaacctcaaa gatgcacccg ccagtatcag cgtcattacc caggaagacc tgcagcgaaa
     8341 accggtacag aatctgaagg atgtcctcaa agaagtgcct ggcgtacaac tgacgaacga
     8401 aggggataac cgtaagggcg ttagtattcg tggtctggac agcagctaca ccctgattct
     8461 cgtcgacggt aaacgcgtta actcccgcaa tgccgtcttc cgccacaatg atttcgatct
     8521 gaactggatc ccggtcgatt ccatcgaacg tattgaagtg gttcgtggcc cgatgtcgtc
     8581 gctatacggc tccgatgcgc tcggcggtgt agtgaatatc atcaccaaaa aaatcggtca
     8641 gaaatggtcg ggtaccgtta ccgtcgatac caccattcag gaacatcgcg atcgcggtga
     8701 tacctataac ggtcagttct ttaccagtgg accattaatt gatggtgtgc tgggaatgaa
     8761 agcttacggc agcctggcaa aacgtgaaaa ggatgacccg caaaactcaa cgaccaccga
     8821 taccggagaa atgccgcgta ttgaaggatt ctccagccgc gacggcaatg tcgaatttgc
     8881 ctggacaccg aatcaaaatc acgattttac tgccggatac ggtttcgacc gtcaggaact
     8941 cgctggacaa aaaccgcctg gaacgccaga actactccgt cagccataat gggcgttggg
     9001 attacggcac cagcgaactg aaatactacg gtgagaaagt cgagaacaaa aaccctggca
     9061 acagcagccc gataacttcc gaaagcaata cggtcgacgg caaatacacg ttgccgctga
     9121 cggcgattaa tcagtttctc acggttggcg gtgaatggcg tcacgacaaa cttagcgatg
     9181 cggtgaacct gaccggggga accagctcca aaacgtctgc cagccagtac gcgctgtttg
     9241 tggaagatga atggcggatc ttcgagccgc tggcgctgac gaccggcgtg cgtatggacg
     9301 atcacgaaac ctacggtgaa cactggagtc cgcgtgccta cctggtttat aacgccaccg
     9361 acaccgtaac ggtgaaaggg ggctgggcga cggcatttaa agcaccttct ctgttgcaac
     9421 ttagccctga ctggacgagc aattcctgcc gtggcgcatg taagattgtg ggtagcccgg
     9481 atctgaaacc agaaaccagc gaaagttggg agctggggct ttactacatg ggtgaagaag
     9541 gctggctgga agggggggaa tccagcgtta ccgttttccg taacgatgtg aaagatcgta
     9601 tcagcatcag ccgtacgtct gacgtcaacg ctgcaccggg ctaccaaaac tttgttggtt
     9661 ttgagacggg cgctaacgga cggcgtatac cggtatttag ctactacaac gttaacaaag
     9721 ctcgtattca gggcgtggaa accgaactga aaattccgtt caacgatgaa tggaaactgt
     9781 cgatcaacta cacctacaac gatggtcgtg atgtcagcaa cggcgaaaac aaaccgctat
     9841 ccgatctgcc gttccatact gctaacggta cgctggactg gaaaccgctg gcgctggaag
     9901 actggtcatt ctatgtttct ggtcactata ccgggcagaa acgcgccgac agcgcgacgg
     9961 ctaaaacacc gggcggttat accatctgga ataccggcgc ggcctggcag gtgactaaag
    10021 acgtcaaact gcgcgcaggc gtgctgaacc ttggcgacaa ggatctcagt cgtgacgact
    10081 acagctataa cgaagacgga cgtcgttact ttatggcagt ggattatcgc ttctgatgag
    10141 aagatgcccg gcgaaccggg cggactttca cttcagtaaa tactgcgcat ggaagcgcag
    10201 gtgatcctct ataaaagagg cgatgaagta gtaactgtga tcatatcccg gctgaatacg
    10261 cagcgtcatc ggccacgctt tctggcgcgc ggcttctgcc agtaccgcag gttgcaactg
    10321 gtcggcaaga aactgatcat tatcgccctg atcgataagc gtcgggatcg catcctgcgc
    10381 gttactggca tacatcagcg cgcaactgtc ccattccagc catgcatttt tgtcctcacc
    10441 taaatagctg ctaaacgctt tgattcccca cgggacgctg cacggattca caattggcgc
    10501 aaaggccgaa acgctggtgt atttacccgg atttttcagc gccataatca gcgcaccgtg
    10561 accacccatt gagtgaccgc taatggcgca gcggtcgctg acattaaatt gcgactgaac
    10621 cagcgccggt aattcatcgc gcagataatc atacatccgg taatgcgtcg cccacggcgg
    10681 ttgcgtggca ttaagataaa agcctgcgcc ctggcctaaa tcgtagccat cgtcgttggc
    10741 aaccttttcg ccgcgcgggc tggtgtctgg catcaccagt acaatcccca gttctgccgc
    10801 cacgcgctgg gcacccgcct tggtggtgaa gttctcgtca ttgcaggtta atccggaaag
    10861 ccagtacagc actggtggcg gggtgtgatc acgagggggt gggagaaaga tactgaacgt
    10921 catcgggcag tttaaggtac tggagtcgtg tcgccagcgt tgctgccagc cttcaaaaca
    10981 gcggtgctct tcgagcattt ccatgcgtgg ctccttgttg tgttgtttgc aatttcttca
    11041 tcataccgtt tattcaatgt gctgtgagta actttcactt ccgtatttgc ataacgatgt
    11101 tttaacatct gctgatgaaa ggcagcggca attacaataa ttatcgctgt gaatactgga
    11161 ttatgtgcgc cgcctcacgc acaataatca ggctgtaaat cagcttaata actttgcccc
    11221 cacgcagggc ggaggcgtca cacctgcagg agaaatcata aatgccatca ctcagtaaag
    11281 aagcggccct ggttcatgaa gcgttagttg cgcgaggact ggaaacaccg ctgcgcccgc
    11341 ccgtgcatga aatggataac gaaacgcgca aaagccttat tgctggtcat atgaccgaaa
    11401 tcatgcagct gctgaatctc gacctggctg atgacagttt gatggaaacg ccgcatcgca
    11461 tcgctaaaat gtatgtcgat gaaattttct ccggtctgga ttacgccaac ttcccgaaaa
    11521 tcaccctcat tgaaaacaaa atgaaggtcg atgaaatggt caccgtgcgc gatatcactc
    11581 tgaccagtac ctgtgaacac cattttgtta ccatcgatgg caaagcgacg gtggcttata
    11641 tcccgaaaga ttcggtgatc ggtctgtcaa aaattaaccg tatcgtgcag ttctttgccc
    11701 agcgtccgca ggtgcaggaa cgtctgacgc agcaaattct tattgcgcta caaacgctgc
    11761 tgggcaccaa taacgtggct gtctccattg atgcggtgca ttactgcgtg aaagcgcgtg
    11821 gcatccgcga tgcaaccagt gccacaacaa cgacctctct tggtggattg ttcaaatcca
    11881 gtcagaatac gcgccacgag tttctgcgcg ctgtgcgtca tcacaactga ttaaaaggca
    11941 ggaaccatgg agcgcaacgt aacgctcgat tttgttcgcg gcgtcgccat tctggggatc
    12001 ctgctattaa acatcagcgc ctttgggcta ccaaaggcgg cttatctcaa tcccgcctgg
    12061 tacggcgcta ttacgccgca ggatgcatgg acctgggcat ttctcgatct catcggccag
    12121 gtgaaattcc tcacgctttt tgcgctgctg tttggtgcgg gcctgcaaat gttgctgccc
    12181 cgtggcagac gctggatcca gtcgcggtta acgctgttag tcttgctggg ttttattcac
    12241 ggtttattgt tctgggacgg cgatattctg ctggcttacg ggctggtggg cttaatctgc
    12301 tggcggctgg tgcgcgatgc gccatcggta aaaagcctgt ttaatacagg cgtcatgctt
    12361 tatctggtgg ggcttggcgt tttgctgtta ttggggctga tttccgacag ccagaccagc
    12421 cgcgcctgga cgccggatgc atcggctatt ttgtatgaaa aatactggaa gcttcacggc
    12481 ggcgttgaag cgatcagtaa tcgtgccgat ggtgttggca acagtttact ggcactgggc
    12541 gcacagtatg gctggcaact ggcagggatg atgctcattg gtgcggcgct gatgcgcagc
    12601 ggctggctga aagggcagtt cagcttacgt cactatcgtc gtactggttt tgtgctggtg
    12661 gcgattgggg tgaccattaa ccttcctgcc atcgccctgc aatggcagct ggactgggcg
    12721 tatcgctggt gtgccttctt acttcaaatg ccgcgggaac tgagtgcgcc gtttcaggcg
    12781 attggctatg cgtcgctgtt ttatggcttc tggccgcaat tgagccgctt taagctggtg
    12841 cttgcgatcg cctgcgtcgg acggatggcg ctgaccaact atctattgca aacgctgatt
    12901 tgtaccatgc ttttttacca cctcggtttg tttatgcatt ttgaccgcct ggagctgctg
    12961 gcgtttgtta ttccggtatg gctggcgaat atcctcttct ctgttatctg gctgcgttac
    13021 ttccgccagg ggccggtgga atggctctgg cgtcagttaa ctttgcgtgc tgccggaacg
    13081 gcaatatcta aaacatcaag ataacgatct ggatcacatt cgttaacaaa acggctgtaa
    13141 ccgtttccat tgctgtgact cgattcacga agtcctgtat tcagtgctga caaaatagcc
    13201 gccagcaagc agtcatttac tgcaatctca taacaggtag tgaatatgat caccattcgt
    13261 gatgtagcgc gtcaggctgg cgtctctgtg gcaacggttt cccgggtgct caataacagc
    13321 acgctggtca gtgccgacac gcgtgaagca gtaatgaaag ccgtgagtga gctggattat
    13381 cggccaaacg ccaatgccca ggcgctggca actcaggtta gcgacaccat tggcgtggtg
    13441 gtgatggacg tttctgatgc gtttttcggc gcgctggtaa aagcggtgga tctagtcgct
    13501 cagcagcatc agaaatacgt gctaatcggc aatagctatc atgaagcgga aaaagagcgt
    13561 cacgccattg aggtgttaat tcgccagcgt tgtaatgcgt tgattgttca ctcaaaagca
    13621 ttgagtgacg atgaactggc gcaatttatg gataacattc ccggtatggt gttaatcaac
    13681 cgcgttgtgc cggggtacgc ccatcgttgc gtttgcctgg ataatctcag cggtgcccga
    13741 atggcgacgc gcatgttgct gaataacggt catcaacgta ttggttatct ttcttccagt
    13801 cacggcattg aagatgacgc catgcgtaaa gcaggctgga tgagtgcgtt gaaagagcag
    13861 gatattattc cgccggaaag ctggattggc actggtacgc cggacatgcc gggcggtgag
    13921 gcggcgatgg ttgaactgct ggggcgcaat ctacaactta ccgctatatt tgcttataac
    13981 gacaatatgg ccgctggcgc actgacagca ttaaaagata atggcattgc gattccgtta
    14041 catctctcaa tcatcggttt cgatgatatt cccatcgccc gttacaccga cccgcaatta
    14101 acgaccgtgc gttatcccat tgcttcaatg gctaaattag ccaccgaact ggccttgcag
    14161 ggggcagcag gcaatattga tcctcgtgcc agccactgtt ttatgccgac gttagtgcgt
    14221 cgccattctg tcgcaacgcg ccagaatgcg gcggcgatca ctaactcaac aaatcaggcg
    14281 atgtaaccgc tttcaatctg tgagtgattt cacagaatct taacaatgtg atagctatga
    14341 ttgcaccgtt ttaacgttgt aacccgtatg taacagtgaa taatcacttt caccgaggta
    14401 atagcgtcat aacaacaatt aaagccgttt tctggagcgt taccgggcat ggaagaacga
    14461 attttaaaaa gtgagcttcg gcgttcagta acacttcatt aactctactg ccctgccgag
    14521 catttatctc aagcactacc ctgcataaga aaaaccggag ataccatgaa taagaaggtg
    14581 ttaaccctgt ctgctgtgat ggccagcatg ttattcggtg ccgctgcaca cgctgctgat
    14641 actcgcattg gtgtaacaat ctataagtac gacgataact ttatgtctgt agtgcgcaag
    14701 gctattgagc aagatgcgaa agccgcgcca gatgttcagc tgctgatgaa tgattctcag
    14761 aatgaccagt ccaagcagaa cgatcagatc gacgtattgc tggcgaaagg ggtgaaggca
    14821 ctggcaatca acctggttga cccggcagct gcgggtacgg tgattgagaa agcgcgtggg
    14881 caaaacgtgc cggtggtttt cttcaacaaa gaaccgtctc gtaaggcgct ggatagctac
    14941 gacaaagcct actacgttgg cactgactcc aaagagtccg gcattattca gggcgatttg
    15001 attgctaaac actgggcggc gaatcagggt tgggatctga acaaagacgg tcagattcag
    15061 tccgtactgc tgaaaggtga accgggccat ccggatgcag aagcacgtac cacttacgtg
    15121 attaaagaat tgaacgataa aggcatcaaa actgaacagt tacagttaga taccgcaatg
    15181 tgggacaccg ctcaggcgaa agataagatg gacgcctggc tgtctggccc gaacgccaac
    15241 aaaatcgaag tggttatcgc caacaacgat gcgatggcaa tgggcgcggt agaagcactg
    15301 aaagcacaca acaagtccag cattccggtg tttggcgtcg atgcgctgcc agaagcgctg
    15361 gcgctggtga aatccggtgc actggcgggc acagtactga acgatgctaa caaccaggcg
    15421 aaagcgacct ttgatctggc gaaaaacctg gccgatggca aaggtgcggc tgatggcacc
    15481 aactggaaaa tcgacaacaa agtggtccgc gtaccttatg ttggcgtaga taaagacaac
    15541 ctggctgaat tcagcaagaa ataagactga ttgggcgcag tcgtttactg cgcccttatt
    15601 gtatggccaa caaggtaaaa ttatatggtc agctcaacga ctccgtcttc cggggaatac
    15661 ttgttggaaa tgagcggtat caacaagtcc tttcctggtg ttaaggcact tgataacgtt
    15721 aatttaaaag tccggccaca ttctatccat gcattaatgg gggaaaacgg tgcaggaaaa
    15781 tcgacattat taaaatgcct gtttggtatt tatcaaaaag actccggcac cattttattc
    15841 cagggtaaag agatcgattt ccattccgcc aaagaggcac tggaaaatgg tatttcgatg
    15901 gtacaccagg agttaaacct ggtattacaa cgttcggtga tggataacat gtggctgggg
    15961 cgatatccca ccaaaggcat gtttgtcgat caggacaaaa tgtaccgcga aaccaaagcg
    16021 atttttgatg aactggatat tgatatcgat ccgcgtgcgc gcgtcggcac attatccgtt
    16081 tcgcaaatgc agatgatcga aatcgccaaa gcgttttcct ataacgcgaa aattgtgatt
    16141 atggatgaac cgacttcttc gttaaccgaa aaagaggtca atcatctgtt cactattatt
    16201 cgtaaattaa aagagcgcgg ctgcggtatt gtttatatct cgcataaaat ggaagaaatc
    16261 ttccagttat gtgatgaagt taccgtattg cgcgacggtc agtggatcgc caccgaaccg
    16321 ctggcaggac tgacgatgga caagatcatc gccatgatgg ttgggcgttc tcttaatcag
    16381 cgttttcctg acaaagaaaa caagccgggc gaagtcatcc tcgaggtgcg taacctaacg
    16441 tcactgcgcc agccgtcgat tcgcgatgtc tcgtttgatc tgcataaagg ggagatcctc
    16501 ggtattgccg ggctggtggg ggcgaaacgt accgatattg ttgagacgtt atttggtatt
    16561 cgcgagaaat cggctggcac cattacgttg cacggcaaac agatcaataa ccataatgcc
    16621 aacgaagcca taaaccacgg atttgcactg gtaactgagg agcgccgctc aacgggaatt
    16681 tatgcctatc tggatattgg ttttaactcg ttaatttcca atattcgcaa ctacaaaaat
    16741 aaagttggtt tactggataa atcgcggatg aaaagcgata cccagtgggt gattgattcg
    16801 atgcgggtaa aaacaccggg gcatcggacg caaattggtt cgctctcagg tggtaatcag
    16861 caaaaggtga ttattgggcg ctggctacta acgcaaccag aaatattaat gctcgatgaa
    16921 ccgacgcgcg gtattgatgt cggggcgaag tttgagatct atcagttaat tgccgaactg
    16981 gcgaagaaag gcaaagggat tattattatc tcctctgaaa tgcctgagtt gttagggata
    17041 acagaccgta ttctggtcat gagcaatggt ctcgtttccg gaattgtcga cacaaaaaca
    17101 acaacgcaaa acgaaattct gcgtcttgcg tctttgcacc tttaagatta ggggcttccc
    17161 atgagtgcgt taaataagaa aagttttctt acttacctga aagagggcgg tatttacgtc
    17221 gttcttttag ttttgctggc gattattatt ttccaggacc caacattttt aagtctgttg
    17281 aacttaagta atattctcac ccagtcatcg gtgcgtatta ttatcgcgct cggtgtggca
    17341 gggttaattg tcacccaggg gaccgacctt tctgcggggc gtcaagtagg gctggcagca
    17401 gtggtggcag cgacattatt gcagtccatg gataacgcca ataaagtgtt cccggaaatg
    17461 gcgacgatgc cgattgcgct ggttattctg attgtctgtg ccattggtgc ggtgatcggt
    17521 ttgatcaacg gtctgattat cgcttatctc aacgttacgc cgttcattac cacgctcggc
    17581 acgatgatca tcgtctatgg catcaactcg ctctattacg actttgtcgg ggcgtcgcca
    17641 atttctggtt ttgacagtgg cttctctacc tttgctcagg gctttgtcgc gctggggagt
    17701 ttccgtctct cttacatcac cttctacgcg ttgattgcgg tggcgttcgt ctgggtgttg
    17761 tggaacaaaa cccgcttcgg taagaacatt tttgccattg gcggtaaccc ggaagcggca
    17821 aaagtatctg gtgtcaacgt cggcctgaac ctgctgatga tctacgcgtt gtctggcgtg
    17881 ttctatgcct ttggcgggat gttagaagcc ggacgtatcg gctctgccac caacaacctc
    17941 ggctttatgt atgagctgga tgctatcgcg gcgtgcgtgg taggcggtgt atcgttcagc
    18001 ggcggtgtgg ggacggtgat tggcgtggtg accggggtaa ttatttttac cgtcatcaac
    18061 tatggcctga cgtatatcgg cgtaaaccca tgctggcagt acatcatcaa aggggcgatt
    18121 attatcttcg ccgtagcgct ggattcactg aaatacgcgc gtaagaaatg atgtgaatgt
    18181 ggtccgcatt cggtcataaa cgcacataac atcatgatct gtgtggtttt gtaggccgga
    18241 taaggcgttc acgccgcatc cggcaacagg agccaaatgc cggatgcaac gctggcgcgt
    18301 tttatccggc ctacaataag ctcaacatac tgatttgtat ggttttgtag gccggataag
    18361 gcgttcaagc cgcatccggc gacaggaacc aaatgccgga tgtgacgcgg gttctacaac
    18421 gttaccgggt gttctttctc gcctttctta aacttcactt ccccgagctc aatacaaccc
    18481 accgggcaga cgtgaccaca aagcagacaa cccacacatt tctcggtatt acaatgcggt
    18541 gtgcgggttt tctcgctcca ttccatcgcc tggtgaccgc cgtcgtaaca ggaaatataa
    18601 cagcgtccac agccaacaca tttgtcaaga ttgatacggg gatagacaat ataactgcgg
    18661 tctaaatctt ccgccgggac aatattgtta ttcgccagac ctaccatctc ctgcaacgaa
    18721 tcaaatccct gatcggcgag ataatgcgat aacccgctcg ccatatcttc tacgatgcga
    18781 ttgccgtact gcataatgcc ggtggtgacc tgtaacgttg ctgcgccgag caataaaaac
    18841 tccgccgcat cctcccaggt ttcaatgccg ccgataccgc tgattgggaa atcgcgcagt
    18901 tctggatggg tgcgcatttg ctgaatgaag cgcagggcga tcggttttac cgctttaccg
    18961 gaatatccgg aaatactcga tttaccgtta acgatcggca taccgatttt ctgattaaga
    19021 tcgatattgg tgatggattt aacggtgtta attgccgcaa tgccatctgc gccgccgcgc
    19081 ttcgccgcca gcgccacttc gcacatatca ccgatattcg gcgtcatctt cgccagcatt
    19141 ggcagcgtgg aaccgcgttt caccgcccga caatattttt ctaccagcac cggactttgc
    19201 ccgacatcgc tacccatcgc atgagaagtc atttgcggac aggagaagtt acactcgatc
    19261 atatccgcgc cagctcctcc cattgctgct cattttcccc catgatcgaa gc
//
LOCUS       DAJTMC010000075        19256 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.075, whole genome shotgun sequence.
ACCESSION   DAJTMC010000075 DAJTMC010000000
VERSION     DAJTMC010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19256
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.075"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(<1..910)
                     /locus_tag="OO130_002284"
     CDS             complement(<1..910)
                     /locus_tag="OO130_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017441594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HCR5365676.1"
                     /translation="MTNFTTSTPHDALFKSFLTHPDTARDFMEIHLPKDLRELCDLDS
                     LKLESASFVDEKLRALHSDILWSVKTREGDGYIYVVIEHQSREDIHMAFRLMRYSMAV
                     MQRHIEHDKRRPLPLVIPMLFYHGSRSPYPWSLCWLDEFAAPTTARKLYSAAFPLVDV
                     TVVPDDEIVQHRRVALLELIQKHIRQRDLMGLIDQLVVLLVTECANDSQITALLNYIL
                     LTGDEARFKAFISELTRRMPHHRERIMTIAERIHNDGWLLGRERGRKEGKVEGERSLL
                     RLLLQNGADPEWIQRYTGLSAEQMQAL"
     gene            1377..2609
                     /gene="mdtM"
                     /locus_tag="OO130_002285"
     CDS             1377..2609
                     /gene="mdtM"
                     /locus_tag="OO130_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtM"
                     /protein_id="HCR5365677.1"
                     /translation="MPRFFARHAATLFFPMALILYDFAAYLSTDLIQPGIINVVRDFN
                     ADVSLAPAAVSLYLAGGMALQWLLGPLSDRIGRRPVLITGALIFTLACAATMFTTSMT
                     QFLIARAIQGTSICFIATVGYVTVQEAFGQTKGIKLMAIITSIVLIAPIIGPLSGAAL
                     MHFVHWKVLFAIIAVMGFISFVGLLLAMPETVKRGAVPFSAKSVFRDFRNVFCNRLFL
                     FGAATISLSYIPMMSWVAVSPVILIDAGSLTTSQFAWTQVPVFGAVIVANAIVARFVK
                     DPTEPRFIWRAVPIQLVGLALLIIGNLLSPHVWLWSVLGTSLYAFGIGLIFPTLFRFT
                     LFSNNLPKGTVSASLNMVILMVMSVSVEIGRWLWFNGGRLPFHLLAVVAGVIVVFTLA
                     GLLNRVRQHQAAELAEER"
     gene            2650..3930
                     /locus_tag="OO130_002286"
     CDS             2650..3930
                     /locus_tag="OO130_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HCR5365678.1"
                     /translation="MNKLIELRRAKMLALSLLLIAAATFVVTLFLPPNFWVSGVKAIA
                     EAAMVGALADWFAVVALFRRVPIPIISRHTAIIPRNKDRIGENLGQFVQEKFLDTQSL
                     VALIRRHEPALLIGNWFSQPENARRVGQHLLQIMSGFLELTDDARIQRLLKRAVHRAI
                     DKVDLSGTSALMLESMTKNDRHQVLLDTLIAQLIALLQRDKSRKFIAQQIVRWLESEH
                     PLKAKILPTEWLGEHSAELVSDAVNSLLDDISRDRAHQIRHAFDRATFALIDKLKNDP
                     EMAARADAVKSYLKEDEAFNRYLSELWGDLREWLKADINSEDSRVKERIARAGQWFGE
                     TLIADDALRASLNGHLEQAAHRVAPEFSAFLTRHISDTVKSWDARDMSRQIELNIGKD
                     LQFIRVNGTLVGGCIGLILYLLSQLPALFPLSNF"
     gene            4046..5198
                     /locus_tag="OO130_002287"
                     /pseudo
     CDS             4046..5198
                     /locus_tag="OO130_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418755.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase subunit D"
     gene            5208..5976
                     /locus_tag="OO130_002288"
                     /pseudo
     CDS             5208..5976
                     /locus_tag="OO130_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313324.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5973..6230
                     /locus_tag="OO130_002289"
     CDS             5973..6230
                     /locus_tag="OO130_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HCR5365679.1"
                     /translation="MKEFLFLFHSTVGVIQTRKALQAAGMTFRVSDIPRDLRGGCGLC
                     IWLTCPPGEEIQWVIPGLTESIYCQQDGVWRCIAHYRVSPR"
     gene            6184..7155
                     /locus_tag="OO130_002290"
     CDS             6184..7155
                     /locus_tag="OO130_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiK family protein"
                     /protein_id="HCR5365680.1"
                     /translation="MVSGAALHITGFLRVKDSLRSLRILLDILLICLKGITVTKSISL
                     SKRISVIVILFAIVAVCTFFVQSCARKSNHAASFQNYHATIDGKEIAGITNNISSLTW
                     SAQSNTLFSTINKPAAIVEMTTNGDFIRTIPLDFVKDLETIEYIGDNQFVISDERDYA
                     IYVISLTPNSEVKILKKIKIPLQDSPTNCGFEGLAYSRQDHTFWFFKEKNPIEVYKVN
                     GLLSSNELHISKDEALQRQFTLDDVSGAEFNQQKNTLLVLSHESRALQEVTLVGEVIG
                     EISLTKGSRGLSHNIKQAEGIAMDASGNIYIVSEPNRFYRFTPKSSH"
     gene            7223..8395
                     /locus_tag="OO130_002291"
     CDS             7223..8395
                     /locus_tag="OO130_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5365681.1"
                     /translation="MPSSSHPVERFSFSTALFGMLVLTLGMGLGRFLYTPMLPVIMAE
                     GAFSFSQLSWIASGNYAGYLAGSLLFSFGAFHQPSRLRPFLLVSALARGLLILAMAWL
                     PPFILVLLIRFLAGVASAGMLIFGSTLIMQHTRHPFVLAALFSGVGIALGNEYVLAGL
                     HFAFSSQTLWQGAGALSGMMLIALTLLMPSKKHAIAPMPLAKTEQQIMSWWLLAILYG
                     LAGFGYIIVATYLPLMAKDAGSPLLTAHLWTLVGLSIVPGCFGWLWAAKRWGALPCLT
                     ANLLVQAISVLLTLASDSPLLLIISSIGFGGTFMGTTSLVMTIARQLSVPGNLNLLGF
                     VTLIYGIGQILGPALTSMLGNGTSALAGATLCGAAALFIAALISTVQLFKLQVVTS"
     gene            complement(8408..8962)
                     /gene="kptA"
                     /locus_tag="OO130_002292"
     CDS             complement(8408..8962)
                     /gene="kptA"
                     /locus_tag="OO130_002292"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HCR5365682.1"
                     /translation="MAKHNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADIDKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGDRHYVHLSADEATARKVGARHGSPVVLTVKAQEMAKR
                     GIPFWQAENGVWLTSTVAVEFLEW"
     gene            9212..9895
                     /locus_tag="OO130_002293"
     CDS             9212..9895
                     /locus_tag="OO130_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5365683.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFSGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGAGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            9892..10353
                     /locus_tag="OO130_002294"
     CDS             9892..10353
                     /locus_tag="OO130_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HCR5365684.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATAGALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            10366..11538
                     /gene="iadA"
                     /locus_tag="OO130_002295"
     CDS             10366..11538
                     /gene="iadA"
                     /locus_tag="OO130_002295"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCR5365685.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPNCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGSKPGVTVFHMGDSKKALQPIYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNLSGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            11603..12514
                     /gene="hypT"
                     /locus_tag="OO130_002296"
                     /gene_synonym="qseD"
     CDS             11603..12514
                     /gene="hypT"
                     /locus_tag="OO130_002296"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HCR5365686.1"
                     /translation="MDDCCAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEYGEAIFNLAQPHFPLLNYSRNSYMGRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNRDELVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(12507..12899)
                     /gene="iraD"
                     /locus_tag="OO130_002297"
     CDS             complement(12507..12899)
                     /gene="iraD"
                     /locus_tag="OO130_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HCR5365687.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(12896..12979)
                     /gene="idlP"
                     /locus_tag="OO130_002298"
     CDS             complement(12896..12979)
                     /gene="idlP"
                     /locus_tag="OO130_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HCR5365688.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
     gene            13571..14401
                     /locus_tag="OO130_002299"
     CDS             13571..14401
                     /locus_tag="OO130_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HCR5365689.1"
                     /translation="MSIPLSNALQSQIITDNHFLHHPKVDNKLTREYEYTRMDTENIY
                     LLPLTRGNNHNYDGKSVVEIRKLNIGKQSWPFNYATDTCREFNGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGYNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIGMGN
                     DPIIGIEAYPGTAEIHAKMGYNVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(14542..15315)
                     /gene="uxuR"
                     /locus_tag="OO130_002300"
     CDS             complement(14542..15315)
                     /gene="uxuR"
                     /locus_tag="OO130_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HCR5365690.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(15530..16990)
                     /locus_tag="OO130_002301"
     CDS             complement(15530..16990)
                     /locus_tag="OO130_002301"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HCR5365691.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLKAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(17071..18255)
                     /gene="uxuA"
                     /locus_tag="OO130_002302"
     CDS             complement(17071..18255)
                     /gene="uxuA"
                     /locus_tag="OO130_002302"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HCR5365692.1"
                     /translation="MEQTWRWYGPNDLVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            18595..18799
                     /locus_tag="OO130_002303"
                     /pseudo
     CDS             18595..18799
                     /locus_tag="OO130_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646216.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(19042..19161)
                     /locus_tag="OO130_002304"
                     /pseudo
     CDS             complement(19042..19161)
                     /locus_tag="OO130_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313306.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
BASE COUNT         4505 a         4900 c         4833 g         5018 t
ORIGIN      
        1 ctaatgcctg catttgctct gccgaaagtc cggtatatcg ttgtatccat tcaggatcgg
       61 ccccattctg caacaacaat cggaggaggc tccgttcccc ttctactttc ccttctttcc
      121 tccccctctc ccttcccaac agccatccat cattatgaat tcgctctgca attgtcatta
      181 ttcgctccct gtggtgtggc attcgcctgg taagttcgct gataaacgcc ttaaaacgcg
      241 cttcatcgcc agtcagtaaa atgtaattta acagcgcagt tatctggctg tcattagcac
      301 actctgtaac cagtaatact accagttggt caataagccc catcaaatcg cgctggcgaa
      361 tatgcttttg gatcaactcc aacagggcga ctctgcgatg ctgcacaatc tcgtcgtctg
      421 gcacgacagt gacatccacc agcgggaacg ctgcgctata aagtttccgt gcggtagtcg
      481 gggcggcaaa ttcgtccagc cagcacaggg accaggggta aggactacgg ctaccgtgat
      541 aaaacagcat cgggataacc agcggtagcg gccggcgttt atcatgctcg atatggcgct
      601 gcatcaccgc catggaatat cgcatcaggc gaaatgccat atgaatatcc tcgcggctct
      661 gatgttcaat cactacgtaa atataaccat caccttcacg ggtctttacc gaccacagaa
      721 tatcggagtg tagcgcccgc aatttttcat cgacaaagct ggcggattcc agttttaagc
      781 tgtcgagatc gcacagttca cgtaaatctt tgggtaagtg gatctccata aaatcccgcg
      841 cggtgtcagg gtgagtgaga aaggatttaa ataatgcgtc atgtggcgtg ctggtcgtga
      901 agtttgtcat ggcgatccgt cacctgtaga taagatggcc cgactttagc gactaacaaa
      961 accattctca cccggcaatt tctcgttccc cgaggtgctt tccaggcgtc tgcaaacgat
     1021 acatttttcc ggaaacaggc tcgcgtttta catttcctgt acccggcaga tattcccctt
     1081 tccaccgcgc tcttttgatt aaccgcaaat tttaattaca cttaaggtgt atattttcta
     1141 tgcaacccat caattaaaga ggtgtaatgt gctgatgact atttgtaatc gttatacatt
     1201 ctgacctgga gtcagaaggt atttctctgt ctgtgtgttc acaggcagtg tggttgatta
     1261 catgaaattc agtacatttg cagtctcgtt gcccttctcg cctgcctttc gtcattaccg
     1321 acggtattga atttcgtttt ccccgttggg gttctccgaa caaggagttg tttgttatgc
     1381 cacgtttttt tgcccgccat gccgccacgc tgtttttccc gatggcgttg attttgtatg
     1441 actttgccgc gtacctgtcg acggatctga tccagccggg gatcattaat gtggtccgtg
     1501 attttaatgc cgatgtcagt ctggccccgg cagccgtcag tctctatctc gcgggcggta
     1561 tggcgttaca gtggctgctg gggccgcttt ccgacaggat tggccgcagg ccggtgctga
     1621 ttaccggggc gctaatattt acccttgcct gcgctgcgac aatgttcaca acgtctatga
     1681 cacagtttct tatcgcgcgt gcaattcagg gcaccagtat ctgttttatt gccaccgttg
     1741 gttatgtcac ggtgcaggag gcgttcggcc agacaaaagg gatcaagttg atggcgatta
     1801 tcacctccat cgtactgatt gcgccgatta ttggcccgct ttccggcgca gctctgatgc
     1861 actttgtgca ctggaaagtc ctttttgcca tcattgcagt tatgggtttt atctcatttg
     1921 ttggcttact gctggcgatg ccagagacgg tgaagcgcgg cgcggttcca tttagcgcca
     1981 aaagcgtctt tcgcgatttt cgtaatgtct tttgcaatcg gctgttcctc tttggcgcag
     2041 caaccatctc tttaagctat atcccaatga tgagttgggt ggctgtctcg ccggtgatcc
     2101 tgatcgacgc gggcagctta acaacttcgc agttcgcctg gacacaggtt ccggtgttcg
     2161 gcgcggtgat tgtcgcgaat gccatcgtgg cgcgtttcgt taaagatccg accgaaccgc
     2221 gctttatctg gcgtgctgta cccattcaac tggtcggcct cgcgctgttg attatcggca
     2281 atctgctgtc accgcacgtc tggctgtggt cggtgctggg caccagtttg tatgctttcg
     2341 ggattggttt gatcttcccg accttattcc gctttacgct tttttccaat aacttaccga
     2401 aagggaccgt ctccgcatcg ctgaatatgg tgatcctgat ggtgatgtct gtctcggtcg
     2461 aaatcggccg ctggctgtgg tttaacggcg gtcgcttgcc gtttcatctg ttagccgtcg
     2521 tggcgggcgt tatcgtcgtt ttcaccctgg caggattgct taatcgcgtg cgccaacatc
     2581 aggctgccga gctggcggag gagcggtgat ttttgcgcga tcctgccgtc aggctctatt
     2641 cttaacgtta tgaataaact cattgaactc agacgcgcca aaatgttggc gctctcttta
     2701 ctgcttatcg ccgctgctac ctttgtcgtt acgctgtttt tgccgcccaa tttttgggtg
     2761 agcggcgtga aggcgattgc tgaagcggcg atggtcggcg cgctggcgga ctggtttgcg
     2821 gtggtggcgc tgtttcgccg cgtgccgatt ccgatcattt ctcgtcatac ggcgattatc
     2881 ccgcgtaata aagaccggat tggcgaaaat ctcggtcagt tcgtgcagga aaaatttctc
     2941 gatacccagt cgctggtggc attgattcga cgtcacgaac cggcgttgtt gattggcaac
     3001 tggtttagtc agccggagaa cgcccgccgc gttggtcagc atctgttgca gatcatgagc
     3061 ggttttcttg aactgaccga tgatgcgcgt attcagcgcc tgcttaagcg cgcggtccat
     3121 cgggcgattg ataaggtcga tctttccgga accagtgcgt tgatgctgga gagtatgacc
     3181 aaaaacgatc gtcatcaggt gctgctcgat acgctgatcg cacagttgat cgcccttctc
     3241 cagcgcgata aatcgcgcaa gtttattgcc cagcaaattg ttcgctggct ggagagcgag
     3301 catccgctga aagccaaaat tttgcctact gaatggctgg gcgaacatag cgcggagttg
     3361 gtttctgacg cggtgaattc tttgcttgat gatatcagcc gcgatcgtgc gcatcagatc
     3421 cgtcatgcgt ttgatcgcgc cacttttgcc ctgatcgaca agctgaaaaa cgatccagaa
     3481 atggcagcgc gagccgatgc cgtaaaaagt tatctgaaag aagatgaagc ttttaaccgc
     3541 tatctcagtg aattgtgggg ggatttacgg gaatggctga aagcggatat caacagtgaa
     3601 gattctcgtg tgaaagaacg tatcgcgcgg gcgggtcaat ggtttggcga aacgttaatt
     3661 gccgatgatg ccttgcgggc atcgttaaat ggtcacctgg aacaagccgc gcaccgcgtc
     3721 gcgcctgagt tttccgcatt cctgacgcgc cacatcagcg atacagtaaa aagctgggat
     3781 gctcgggata tgtcgcggca aatcgagtta aatatcggca aagatctgca gtttatccgt
     3841 gtcaacggta cgctggttgg cggttgtatt gggctaattt tatatttgtt gtcgcagctc
     3901 ccggccttgt tccccctcag caatttttag aaatccatga aaaactaagt ggctaaccgc
     3961 gagagagatc aaataaacca cactaaaaga atggtaataa tgtggcctct tttcattatc
     4021 tcccgtggta cggggaagga aaatcatgtc acttatcacc gatctacccg ccattttcga
     4081 tcagttctct gaagctcgcc agaaaggctt tctcaccgtc atggatctca aggagcgcgg
     4141 cattccgctg gttggcactt actgcacctt tatgccgcaa gagatcccga tggcagccgg
     4201 tgcggttgtg gtttcgctct gttctacctc tgatgaaacc attgaagaag cggagaaaga
     4261 tctgccgcgc aacctctgcc cgctgattaa aagcagctac ggcttcggca aaaccgataa
     4321 atgcccctac ttctactttt cggatctggt ggtcggtgaa accacctgcg acggcaaaaa
     4381 gaaaatgtat gaatacatgg cggagtttaa gcccgttcat gtgatgcagt tgccgaacag
     4441 cgttaaggac gatgcctcgc gtgcgttatg gaaagccgag atgctgcgct tgcaaaaaac
     4501 ggtagaagaa cgttttgggc acgagattag cgaagatgct ctgcgcgatg ccattgcgct
     4561 gaaaaaccgc gaacgtcgcg cactggccaa tttttatcat cttgggcagt taaatcctcc
     4621 ggcgcttagc ggcagcgaca ttctgaaagt ggtctacggc gcaaccttcc ggttcgataa
     4681 agaggcgttg atcaatgaac tggatgcaat gaccgcccgc gttcgtcagc agtgggaaga
     4741 aggccagcga ctggacccgc gtccacgcat tttaatcacc ggctgcccga ttggcggcgc
     4801 agcagaaaaa gtggtgcggg cgattgaaga gaatggcggc tgggttgtcg gttatgaaaa
     4861 ctgcactggg gcgaaagcga ccgagctaat gcgtggcgga aacgggcgat gtctacgacg
     4921 cgctggcgga taaatatctg gcgattggct gctcctgtgt ttcgccgaac gatcagcgcc
     4981 tgcaaatgct cagccagatg gtggaagaat atcaggtcga tggcgtagtt gatgtgattt
     5041 tgcaggcgtg ccatacctac gcggtggaat cgctggcgat taaacgtcat gtgcgtcagc
     5101 agcacaacat tccttatatc gctattgaaa cagactactc cacctcagat gtcgggcagc
     5161 tcagtacccg tgtcgcggcc tttattgaga tgctgtaagg agtggcagtg acatattcga
     5221 ttggcattga ttctggttct accgccacca aagggatctt actggcagac ggcgtgatta
     5281 cgcgccgttt cctcgttcca acaccctttc gtccggcaac agcaattact gaagcctggg
     5341 aaactctgcg cgaagggtta gagacaacgc cgtttctgac gctcaccggc tatggacggc
     5401 aactggtgga ttttgccgat aaacaagtga cggaaatctc ctgtcacggg ctgggcgcac
     5461 ggtttcttgc gccagcaacg ctgcgcggta atcgacatcg gtggtcagga cagcaaagtg
     5521 attcagcttg atgatgacgg taacctgtgc gatttcctga tgaatgacaa atgcgcggcg
     5581 ggcaccgggc gtttcctgga agtgatctcg cgcacgcttg gcaccagcgt cgagcaactc
     5641 gacagcatta ccgaaaatgt cacgccgcat gccatcacga gtatgtgcac agtgtttgcc
     5701 gaatcagaag tgatcagcct gcgctcagcg ggcgtcgcgc cagaagcgat tctcgccgga
     5761 gtgattaacg cgatggcgcg gaggagtgcc aatttcattg ctcgtctctc ctgtgaagcg
     5821 ccgattctgt ttactggtgg cgttagtcat tgccagacgt ttgcccggat gctggaatct
     5881 cacctgggaa tgccggtaaa tacccatcct gatgcgcaat ttgctggcgc aattggcgcg
     5941 gcggtaattg gtcaacgagt gaggacacgc cgatgaaaga gtttttattt ttatttcact
     6001 ccacagtcgg cgtcatacaa actcgcaaag cgttgcaggc agcgggcatg acttttcgcg
     6061 tcagcgatat tccccgtgat ttacgcggcg gctgcgggtt atgcatttgg ctgacctgtc
     6121 cccccggcga agaaatacaa tgggtgatcc ctgggctgac tgagtcaatt tattgccagc
     6181 aggatggtgt ctggcgctgc attgcacatt accgggtttc tccgcgttaa ggattcatta
     6241 aggtcgctac gtatacttct cgatatctta ttgatttgcc ttaaagggat taccgtgaca
     6301 aaaagtattt cgttaagtaa acgcatctcc gttattgtga ttctttttgc cattgtggca
     6361 gtgtgtactt tcttcgtgca atcttgcgcc cgtaaaagca atcatgcagc atcatttcag
     6421 aattaccacg ccaccattga cggcaaagaa attgcaggca taacgaataa tatctcttcg
     6481 ctgacctggt cggcacaaag caatactctt tttagcacta tcaacaaacc tgccgccatt
     6541 gttgaaatga ccaccaatgg tgattttatc cgcacaattc ctctggattt tgttaaggat
     6601 ctcgagacga tcgaatacat tggcgataat cagtttgtca ttagcgatga gcgcgattac
     6661 gccatttatg tgatttcact gaccccaaac tcggaagtaa aaatcctcaa aaaaataaaa
     6721 atcccgctgc aagactcccc aaccaattgc ggttttgaag ggttagctta ttctcgccag
     6781 gatcacactt tctggttttt taaagagaaa aatccgattg aggtctacaa agttaacggg
     6841 ctgttaagta gcaatgaatt acacatcagc aaagacgagg cactacaacg gcaatttact
     6901 ttagatgatg tgtccggcgc agaatttaac cagcaaaaaa atacgcttct ggtgctgtct
     6961 catgaatctc gcgcactcca ggaagtaacc ctggttggcg aggttatcgg tgaaatatca
     7021 ctaaccaaag gtagtcgagg tttgtcgcat aacatcaaac aggccgaagg gattgcgatg
     7081 gatgcctctg gtaatattta tatcgttagc gaaccaaatc gtttttatcg ctttacccct
     7141 aaatcatcgc attaacaggc tggcatattt cacctttttg ttattaactt gttaacaaca
     7201 aaaatgacat acaaggttaa acatgccttc gtcctcgcat cccgtagaac gcttttcttt
     7261 cagcaccgcg cttttcggga tgctggttct gaccctaggt atgggattag gtcgcttcct
     7321 ttatacgcct atgttgcccg tcattatggc ggaaggggcg ttttcattta gccagctctc
     7381 gtggattgcc agcggcaact atgcggggta tctggctggc agtctgctat tttcgtttgg
     7441 cgcatttcac cagccgtcgc gcctacgccc atttctgttg gtttctgccc tggcgagagg
     7501 gttgttgatc ctcgcaatgg catggttgcc gccatttatt ctggtgttat tgattcgctt
     7561 cctggcgggt gtcgccagcg ccggtatgct gatttttggt tcgacgctga ttatgcagca
     7621 cacgcgccat ccttttgtgc tggcagcttt gttttctggc gttggcattg cactgggcaa
     7681 cgaatatgtt ctggcaggcc tgcattttgc cttctcctcg caaacgttat ggcaaggcgc
     7741 cggggcgctt tctggcatga tgctgatcgc acttacgctt ttaatgccct cgaaaaaaca
     7801 cgccatcgca ccaatgccat tggcaaaaac ggagcaacag ataatgagct ggtggttact
     7861 ggctattctg tacggccttg cgggatttgg ttatatcatc gtcgcgactt acctgccgct
     7921 catggcgaaa gatgcaggct caccattgtt aaccgcccat ctctggacgt tagtcggctt
     7981 atcaattgtg ccaggctgct ttggctggct atgggcagca aaacgttggg gggctttgcc
     8041 ttgcctgacg gcgaatttgc tggtgcaggc tatcagtgtg ctgcttactc tcgccagcga
     8101 ctctcctctc ttgcttatca ttagtagtat cggttttggc ggtaccttta tgggcacgac
     8161 atctctggtg atgactatcg cccgccaact gagcgttccg ggaaatctca atcttttagg
     8221 ctttgtgacg ctcatttacg gcattggaca aatcctcggc ccggcgctga ccagtatgct
     8281 cggcaatgga acatcggcgc ttgccggcgc cactctctgc ggcgcggcgg cgttgtttat
     8341 tgctgcgctt atcagcacag tacaattatt taagctgcaa gtggtcactt catgaggagt
     8401 gtttccctta ccactcaaga aattcgaccg caaccgttga tgtcagccag actccgtttt
     8461 ccgcttgcca gaaaggaatg cctcgtttcg ccatttcctg tgctttgacg gttaagacaa
     8521 ccggcgatcc gtgtcttgct cccactttgc gtgccgttgc ttcatcagca gagagatgca
     8581 cgtaatgacg atcccctgca atcagcccct gtttttttat ctcgtcaagg aaacggcttg
     8641 ccgtaccgtg gtagagaaac tgtggcggtg ttttttcggc aaaggaaata gcgacctgcg
     8701 aagtcgaatg cccctgaacc gcgcggatgc acctaccatc actggaataa ctaaaacgct
     8761 ttttatcgct ggttgcgacc acggtatcca gcaatgcacg ggttaatcgc ttgcccgctt
     8821 tttgggcaca aagtatgagc ttatcgatat ccgcccatcc ttcacggtcc agtacgatac
     8881 caatcgcctc cggtttgtgt cggaggacaa aacttaaaaa cttgctcgta tccgccagct
     8941 ctttttcgtt gtgctttgcc atctcgtttt cgtccctgga ttttctgttt ttatttacca
     9001 atgtcattgt cactgggttt atgaattttc tgcaactcaa tcctgcgcag aaccttttcc
     9061 acacagcgta atgtttcccc tatgcaaaaa acgcatagcc agaaacatac ttgcattttc
     9121 cccataccca catggctgat aaggtttttc agttccagga attttcctgg ctcaagcaac
     9181 acacaaacac aaaaaaacaa tggggattgt tatgacacaa caaggggatg ctgtagccgg
     9241 ggagctggcg acagaaaaag tgggcattaa agggtatctg gctttttttc tcaccataat
     9301 ctttttttcc ggtgtttttt ccggaacaga cagctggtgg cgcgtttttg atttttccgt
     9361 tctgaacgga tcgtttgggc aactgcctgg tgcgaacgga gccaccacct catttcgcgg
     9421 cgcgggtggt gcgggggcta aagatggctt tctctttgcg ctggagctgg caccgtcagt
     9481 cattctctct ctggggatta tttcaattac cgatggtctt ggtggattac gcgccgcgca
     9541 gcagttaatg acacctgtgc taaaaccgtt gttgggcatt ccagggatct gctcgctggc
     9601 gctgatcgct aacttgcaaa acactgatgc cgccgccggt atgaccaaag agctggcgca
     9661 ggaaggtgaa attaccgaac gcgataaagt gattttcgcc gcatatcaaa ccagcggtag
     9721 cgccatcatc accaactact tctcttccgg cgtcgcggtg ttcgcctttc tgggcacgtc
     9781 cgtaatcgta cctttagccg tcatcctggt gtttaaattt gtcggagcca acattttgcg
     9841 cgtctggctt aacttcgaag aacgtcgcaa cccaacgcaa ggagcgcaag catgacaact
     9901 caggtccgca aaaacgtcat ggatatgttt atcgatggcg cgcgccgtgg ctttactatc
     9961 gccacgacca acctgctgcc aaacgtggtg atggcgtttg tcatcattca ggcgctgaaa
    10021 attaccggcc tgctcgactg ggtggggcat atctgtgaac ccgtaatggc gctgtgggga
    10081 ttacccggtg aagcggcaac cgtcctttta gccgcattaa tgagtatggg gggcgcagtc
    10141 ggtgttgccg ccagtctggc aaccgctggc gcattaaccg gtcacgatgt caccgtttta
    10201 ctgcccgcga tgtatttaat gggcaacccg gtgcaaaatg tcggtcgctg cctggggaca
    10261 gccgaagtga atgccaaata ttacccgcat atcattacgg tctgcgtgat taacgcattg
    10321 ctgtcgatct gggttatgca gcttattgtt taacaaggag ttaccatgat tgattatacc
    10381 gcagccggtt ttaccctgct gcagggagcg catttgtatg cgccggaaga tcggggaatt
    10441 tgcgatgtcc tcgtcgctaa cggcaaaatt atcgccgttg ccagcaatat cccttctgac
    10501 attgtaccga actgcacggt tgtcgatctc agtgggcaga tcctctgccc aggttttatt
    10561 gatcaacacg tccatttgat tggcggtggc ggcgaagcag gtcccacgac gcgcacgccg
    10621 gaagtggcgc taagtcgcct gacggaagcg ggcgtcacgt cagtggttgg tctgctgggc
    10681 accgactcta tctctcgcca cccggaatcc ctgctcgcca agacccgtgc gctcaatgaa
    10741 gaaggcatca gcgcctggat gctgaccggc gcttatcatg tcccttcccg cactattacg
    10801 ggttccgtgg aaaaagacgt ggcgattatc gatcgtgtga ttggcgtgaa atgcgccatc
    10861 tctgatcacc gttctgccgc accggacgtt tatcacctgg ctaatatggc ggcagaatcc
    10921 cgcgttggcg gtttgctcgg cagtaaacct ggcgtcaccg tgttccacat gggcgacagt
    10981 aaaaaggcgt tacagcctat ttatgacctg ctggaaaact gcgatgtgcc gatcagcaag
    11041 ctgctgccga cccacgttaa ccgcaacgta ccgttgtttg agcaggcgct ggagttcgcg
    11101 cgcaaaggcg gcaccatcga tatcaccagc agcattgacg aaccggtcgc ccctgccgaa
    11161 ggtattgccc gcgccgttca ggcgggtatt ccgctggcac gcgtcaccct cagctccgac
    11221 ggcaacggta gccagccgtt cttcgatgac gaagggaatt taacccatat cggtgttgca
    11281 ggttttgaaa cgttgctgga aaccgtgcag gtgctggtca aagactatga tttcagtatc
    11341 agcgatgccc tgcgcccgct caccagtagc gtagccggtt tccttaacct gagcgggaaa
    11401 ggcgaaattc tgcctggcaa tgacgcagac ttactagtca tgacgccaga attgcgcatt
    11461 gagcaggtat atgctcgcgg caaactgatg gtcaaagacg gcaaagcctg cgtgaaagga
    11521 acgtttgaaa cggcttaaac ggttgtcagc cgccctaatg gacgtagaat gcccgcaagg
    11581 gcggctgaca gagtaaaacg taatggatga ctgttgtgcg gttttgcata atattgaaac
    11641 caaatggctt tatgattttc tgaccctgga aaaatgccgc aatttttccc aggcggcagt
    11701 cagtcgtaac gtctcgcaac cggcattcag ccgccgcatc cgcgcgctgg aacaggcgat
    11761 tggcgttgaa ttgtttaacc gccaggtgac gccgctgcaa ctctcagaac aagggaaaat
    11821 cttccattcg cagatccgcc atctgttgca acagttagaa agtaaccttg cagagctgcg
    11881 tggcggcagc gattacgcgc aacgtaaaat caagatagcc gctgcacact ctctttccct
    11941 cggtctgtta ccgtccatta tcagccagat gccgccgctc tttacctggg cgattgaagc
    12001 tattgatgtc gatgaagcag tcgataaact gcgtgaaggg caaagtgact gtattttttc
    12061 ctttcacgac gaagatttgc tggaagcacc gtttgaccac attcgcttat ttgaatctca
    12121 attgttcccc gtttgcgcca gtgacgaata cggagaagca atttttaacc tcgcgcagcc
    12181 acactttccg ttactgaatt acagccgcaa ctcctacatg gggcgattga ttaatcgcac
    12241 cctgacgcgc cacagcgagt taagtttcag cacctttttt gtctcttcga tgagcgagct
    12301 attaaagcag gttgccctcg acggctgtgg gattgcctgg ctgccggagt atgccatcca
    12361 acaagaaatt cgcagcgggc aactcgttgt gctgaatcgg gacgaactgg tgatcccgat
    12421 tcaggcctac gcataccgta tgaatacccg catgaatcca gttgccgaac gcttctggcg
    12481 tgaactgcgc gagctggaga ttgtgcttag ctgacattct caagcgtcgc actgcgccaa
    12541 ccgccattat cccagaccag atcaaacgtt agcggttcat cgtcgtggta cagcattatc
    12601 tcaaggctga tcgccagagt gtggcagtac gcttctttca aattaacctg caacgccgca
    12661 atccgtggct catgaaataa cagtgcagaa cgcagctgac ggcaatacca gctctggaga
    12721 tgtttgtcgc tcgggttatg cacttgccac tcgggcatgc ctgaaacaag taatgacggt
    12781 aatgccgaat gaggagaatt aaaaagagtg aggagatcgc tgcataccga tttacgcagc
    12841 aatgacgttt tattgcctga aatttcaggt aaaacagcct gaagtgattg gcgcatcatt
    12901 ttgcgcactc ctgacgttta gcaacatatg cggcttgccc tgaacaccgg gcaccactgt
    12961 attgatgctc attttccata gccattccct tttgttcatt acatccatgt cagtctgaga
    13021 caggcctgaa tcctgtcagc aaagtataaa accttacttg cctatgaata tctactgact
    13081 tttcaggaaa aaagtcatga tttgataaaa atcatcaaac tcaaaaaaaa accgctagta
    13141 tattctgaat attccgaata gttacgtaga tgaaaatact tgtcaccgcc gctacttaat
    13201 tccggtggaa aattatttat ttccgcataa tcagccatgt attatttgaa acaattagtt
    13261 tgtaaataat ataaattgat aacatatcaa atacatcaaa aataccattc ataggcatta
    13321 ttaatataga catcaatata gcaacatcaa attccaatta gtaataagat atgcttttta
    13381 attgcatgaa aataatttca aatgagcgtt ttttgatact ttagagtgtt tcctaccctt
    13441 taatatcacc gtgtagagtt gatatcacta caacagaaac atgcatgtca tttttttact
    13501 tcttttttac tacagaaaaa tttatctatt tttctaaaca tcggtgaaat actcataaaa
    13561 ggtaactatt atgtctatac cattaagcaa tgcccttcag agtcaaatta ttacagataa
    13621 tcacttcctt catcatccaa aagtcgacaa taagttaaca cgtgaatatg aatatacacg
    13681 catggatacg gaaaatatct atcttctgcc tcttactcgt ggaaataacc ataattacga
    13741 tggaaaaagc gttgtcgaaa ttagaaaact taatattggc aaacagtcct ggccatttaa
    13801 ttatgcgaca gatacatgca gagaattcaa tggtattaca acaacaggac gtatgctcta
    13861 tcgaaacttg aaaatcacct cagctttaga cgaaatatat ggtggcatat gtaaaaaggc
    13921 gcatgctgct acagagttag cagaaggttt acggctaaat ttatttatga agagcccttt
    13981 cgatcctgtt gaggactata cagttcatga aataacatta ggccctggtt acaatgttcc
    14041 tggttatgcc ggaaccacga taggatatat ttcaaccctt ccagcttctc aggcaaaaag
    14101 atggaccaat gagcagccaa gaattgatat ctatattgat cagataataa ctgtctcagg
    14161 ggtggcaaat tcttccggat tcgcccttgc cgcattactt aatgctaata ttggaatggg
    14221 aaatgaccca ataattggta tagaagccta tcctggtacc gcggagatcc atgcaaaaat
    14281 gggatataac gttatccccg gtgatgaaga tgcgccactc aaacgcatga ctctgcaacc
    14341 gtcatctctt ccagaactgt ttgaattaaa aaatggtgaa tggaattata ttggcaaata
    14401 accactgaga agaatgagta ctaacattca ggttatatag atatttaaac aataccctgc
    14461 aatttacaaa gtaatactca ttgcgacttc ttaatttaag aaagcgcaag gaacgtttac
    14521 ccttgcgctt atcataatca gtcaggcgtc gactttatcc agcggccatg aatcaaacag
    14581 atagccgtca aaataaatat cgtcaacgtt cgagaattcc agcagacgtt gcttaacgtt
    14641 ttccagatgc tgccacattg ccagcttcgc cgctcgggca tcttttttga ttaacgcggc
    14701 aaggatctgt ttgtgatcgc ccaaccactc tttgcgatac aggctgtcat ccagatggct
    14761 gtgcaactgg atccacattg gattgttttc ccgccactgc caggactgac ggaataactc
    14821 caccagcatg ctgttatgcg ttgcttcagc aatggcgaga tggaactgca tgtcaccgct
    14881 ttcgctgcta cccggcgcgc tggaagccag ttcacgctct tccagttgca atgcctgacg
    14941 cattttgacg atatcttcgc gggtagcctg cagggcggca aattccgcga tgttgctctc
    15001 aagaagctgt cgcgcctgca acaattcaaa ggggccggca tcattgcaga cgttggcatc
    15061 cggactgtct gtgttgtggc tgcctgagct atcaagaaca tagataccgg caccccggcg
    15121 cacttccacc agccctttga tctccagcat gatcagcgct tcacgtacca ccgtccgcgt
    15181 gacatcaagc atttctgcaa tttcacgctc cggcggcagc cgttcgccag gattgtacgg
    15241 cgtctttatg atcagatcgc ggatcatcgc cccgacttcc tggtaaggtc tttgcgcaga
    15301 ggtggcagat ttcatggtgt tgtgtccgtt aggtgcggtt aatcaaattt ttgccggatg
    15361 cggcgtgaac gccttatccg gcctacaacg ggcaatttta cggcccccgt taatctcaaa
    15421 ctataccatt agcactggaa gttggcacgt acaggtgggc aaaaaaacga aaactggtca
    15481 accaaatatc agtttgattg accagtaagt ctgtagggta atcagttagt tacagcgcag
    15541 ccacacactc gcgcgcaccg cgttcgcaca gctgctgata tgccgccgtc accgcgccaa
    15601 caaagtcggc attctgcggc agatcatcgg caaaaatacc gctcaggccc agcagcgctt
    15661 tcacgcggtc tgcgccctga tactgcgcgt taatcttctg gaactcggcc agcatcgggt
    15721 cgaccacgtc aatggcatta ccctgctcat ccacgccctg ggtgtaacgc atccagccag
    15781 ccacgcccag cgccaggtga cgccagctgc cgccgttttg caggtgcaga cgcaccgggt
    15841 ccagcagacg ctgcggtaac ttctggctgc cgtccatcgc aatctgccag gtacggtgac
    15901 gcagagacgg gttgctgaaa cgctcgatca gcagcgtcgc ataggccttc aggtccgtac
    15961 cctccggcat agacagcgtt ggcgcttgtt cctgcatcat cagggcaaag gccgctttgc
    16021 gataatccgg gttagtcatg gtgtcggcaa tagtttcata gccgccgagg taaccgaggt
    16081 atgccaggaa agagtggctg ccgttcagca tacgcagctt catcatttcg aacggcacaa
    16141 cgtctgcaac gaactgtgcg cccactttat cccagtccgg gcgaccatta acgaagttat
    16201 cttcaatcac ccactgacgg aacggttcgc aggcaatggc gcacgggtcg taaacaccca
    16261 gctggtcagc aatttcctgt aaggtttctg gcgtcgccgc cggaacgatg cggtcaacca
    16321 tggtgcacgg gaaggtgacg ttctcttcaa tccatgccgc cagctgcggg tcacgtgcct
    16381 gagccagccc cagtaccgcg accttcgcca catgaccgtt ttcacgcacg ttatcgcagg
    16441 acatcaccgt aaatgctttc agcccttttt cacgacgcag acgcaaggct tcgacgatgt
    16501 aaccaatcgc ggacttcggc gcagtcgggt tttctagatc gtgcttgatc agcgggttat
    16561 tgagatccag ttgaccgctt gccgcatcag cgcagtagcc tttttccgtg accgttagag
    16621 agacaatcgc cgtttgcgga cgcgccatcg cgttgagaat accttcgcag ccgtcgattt
    16681 ccggatgcag cgcttctttc atcgaaccga taattttcag ctcggtgctt tctgcgcctt
    16741 tttccgctac ggtgtacagc agttgctgtt ttttcaggtt ttcgatcagc acgcggtcgt
    16801 tgcctggcat caggttaact tcgcagatgc cccagtcgct gtcggtgctt tccagcagat
    16861 gatgggtata cagcgcctga tgtgcgcggt gaaacgcccc gcaaccgaga tgcacaatgc
    16921 gtgattccag acgagaatga tcccatgacg gacgggcaac cggcagattg ctgtcaacaa
    16981 tagtagtcat cgtaaactcc agaatccgtc ttcagagagt gacggcaagg aagaaggaac
    17041 cggcacggcg cgcagccatg ccggtggata ttaacggcta aagaaagcgc gctggatcgc
    17101 cagttcgaca ccgcgaactt cggccaggcc tttcagacga ccaattgcgg agtaacctgg
    17161 gttggttttc ttcttcaggt cgtccagcat ctgatgaccg tggtccggac gcatcgggat
    17221 caggtcttct ttgccttccg ctttacgacg gtgttcttct tcaacaatcg ctttcaccac
    17281 ttcgtacata tcaacgtcac cgttcaggtg cgccgcttcg tggaaggttt tcgggttatc
    17341 ttcacgcatg gtggagcgca gatgggtgaa gtaaatacgc ggaccgaact gcttgatcat
    17401 atcaaccaga tcgttgtcag cacgcacgcc gtaggaaccg gtgcacatgg tgaaaccgtt
    17461 cgccatgctg tttacggtat caaccatcca ctgcatatct tcaatggtgg aaacaatgcg
    17521 cggcaggccg aggatcgggc gcggcggatc gtccgggtga accgccatac gcacgccaac
    17581 ttcttcagca actggaataa tcgctttcag gaagacggca aagttttcgc gcagcttcgc
    17641 tttatcgata tctttgtaca gctccaggtg cttacggaac tggtcgaggg tatacccttc
    17701 ttccgcgccc ggcagaccgg caatgatgtt acgcgtcaga cgcgctttgt cttcatcgct
    17761 catagtggcg aagcgttcag ctgcctgagc aatttcttct tcggtgtaat ccgcttccgc
    17821 gcctggacgt ttcaggatat gcatttcgaa tgcagcgaat tcgatctggt cgaagcgcag
    17881 agctttggag ccgtctggca gcacgtattc gaggtcagta cgggtccagt cgagcaccgg
    17941 catgaagttg tagcacacgg tgcgaatgcc gcactgcgcc aggttgcgca gagtctgctg
    18001 atagttagcg atccactgct catagttgcc agtgtgggtt ttgatatctt cgtgaattgg
    18061 tacgctttct acgacagacc acaccaggcc tgcgtcttca acgatcgcct tgcgtttgag
    18121 gatctcttct acggaccata cttcgccgtt cgggatatgg tgcagcgcgg taaccacgcc
    18181 agttgcgccc gcctgacgga catcagctaa agaaaccaga tcgtttgggc cgtaccagcg
    18241 ccaggtctgt tccatgattt ttcctctttc acatagtggg ttccacagca aagagacgcg
    18301 gaaccctgga gttgtttagg tatccggatg aacaaccaga ccgcagccgt ggcgttgcat
    18361 cggatgagat ggcgtataag ttctaccttt tggtaagata tgtcaaccca aatcctgaaa
    18421 ttggttaacc aaatcacaag aatttcactc tcatggttgt tgctgcaaat tgcagcaaaa
    18481 ggtaatcgat ggttattgat cagaggatgt gacattcatc gcaacaatgg ttgaccaatt
    18541 tacataacat gtcgcccaaa ataacaacgg ttcaaccagg taaggtaaaa cgttatgcat
    18601 gtgcttaaca ttctctgggt agtattcggc attggtctga tgctggtact gacaacgacc
    18661 gcaggttcag acacattcac tgcattagca atgcctctta gattattttg acctgtcagt
    18721 aaaagacacg ttgaaaacct ggggtctgct ggaactggtc aactccgtgg ttgggctgat
    18781 tattagcaag gtagcgtaaa agaacaccag agcctgccaa tggcaggctc tgactgatga
    18841 caaagtctaa aaatgtgccc ggacagtccc ctcgcccctc cggggagagg gttagggtga
    18901 ggggaacaga ccagcaaagt gcgctttgtt tatgccagat gcgacgctga cgcgtcttat
    18961 ctggcctaca aagggctaac gtgcaggttt ttagcttcag gtaatattgc gtaactgcat
    19021 tagcaatgcc ctgtgatttc tttattgata aacaaaagtc acgccaataa tcgattgcac
    19081 attccctgca gtcacctgcc ctccggtacg tgcgtaattt gccgttaatc ccagacttac
    19141 cgccgaagtt cctactgaac cgccccggga atcctggaga ctaaactccc tgagaaagag
    19201 gtaaacagga tgactaaaaa tactcgtttt tcccccgaag tccgtcagag ggcgat
//
LOCUS       DAJTMC010000076        19086 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.076, whole genome shotgun sequence.
ACCESSION   DAJTMC010000076 DAJTMC010000000
VERSION     DAJTMC010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19086
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.076"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(31..2478)
                     /gene="glgP"
                     /locus_tag="OO130_002305"
     CDS             complement(31..2478)
                     /gene="glgP"
                     /locus_tag="OO130_002305"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HCR5365693.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(2497..3930)
                     /gene="glgA"
                     /locus_tag="OO130_002306"
     CDS             complement(2497..3930)
                     /gene="glgA"
                     /locus_tag="OO130_002306"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HCR5365694.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRASLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(3930..5225)
                     /gene="glgC"
                     /locus_tag="OO130_002307"
     CDS             complement(3930..5225)
                     /gene="glgC"
                     /locus_tag="OO130_002307"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HCR5365695.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLKAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(5243..7216)
                     /gene="glgX"
                     /locus_tag="OO130_002308"
     CDS             complement(5243..7216)
                     /gene="glgX"
                     /locus_tag="OO130_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HCR5365696.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGSLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            complement(7213..9399)
                     /gene="glgB"
                     /locus_tag="OO130_002309"
     CDS             complement(7213..9399)
                     /gene="glgB"
                     /locus_tag="OO130_002309"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HCR5365697.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDASISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPVYRQYHHDKLT
                     FGILYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(9672..10775)
                     /gene="asd"
                     /locus_tag="OO130_002310"
     CDS             complement(9672..10775)
                     /gene="asd"
                     /locus_tag="OO130_002310"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCR5365698.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            10967..11560
                     /gene="yhgN"
                     /locus_tag="OO130_002311"
     CDS             10967..11560
                     /gene="yhgN"
                     /locus_tag="OO130_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HCR5365699.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(11617..12957)
                     /gene="gntU"
                     /locus_tag="OO130_002312"
     CDS             complement(11617..12957)
                     /gene="gntU"
                     /locus_tag="OO130_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCR5365700.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGPLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(12961..13488)
                     /gene="gntK"
                     /locus_tag="OO130_002313"
     CDS             complement(12961..13488)
                     /gene="gntK"
                     /locus_tag="OO130_002313"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HCR5365701.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMLRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(13627..14622)
                     /gene="gntR"
                     /locus_tag="OO130_002314"
     CDS             complement(13627..14622)
                     /gene="gntR"
                     /locus_tag="OO130_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HCR5365702.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHCHIAYLGARLDERTIIKQKGYEQAMLDAGLVSYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(14846..15541)
                     /gene="yhhW"
                     /locus_tag="OO130_002315"
     CDS             complement(14846..15541)
                     /gene="yhhW"
                     /locus_tag="OO130_002315"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HCR5365703.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(15664..16701)
                     /locus_tag="OO130_002316"
     CDS             complement(15664..16701)
                     /locus_tag="OO130_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR5365704.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFISDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLSVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     PEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAN"
     gene            17033..17521
                     /gene="yhhY"
                     /locus_tag="OO130_002317"
     CDS             17033..17521
                     /gene="yhhY"
                     /locus_tag="OO130_002317"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR5365705.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRSSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKYGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
     gene            17758..18957
                     /locus_tag="OO130_002318"
     CDS             17758..18957
                     /locus_tag="OO130_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp1 family type VI secretion system effector"
                     /protein_id="HCR5365706.1"
                     /translation="MSNIVYLTVTGEQQGSISAGCGTSESTGNRWQSGHEDEIFTFSL
                     LNNINNTGLGSQFHGITFCKLIDKSTPLFINSINNNEQLFMGFDFYRINRFGRLEKYY
                     YIQLRGAFLSAIHHQIIENQLDTETITISYEFILCQHLIANTEFSYLALPENYNRLFL
                     PNSKNQTNNRFKTLNSKAIGRLLAAGGVYNGNIEGFRDTAEKLGGDAIKGYDQILNEK
                     TAGIAIATASILLTKRSNVDTYTEINSYLGKLRGQQKLLDGIDIIEIIYIKRPSKDLA
                     NLRKEFNKTVRKNFLIKLSKTSEASGRFNAEDLLRMRKGNVPLNYNVHHKLSLDDGGT
                     NDFENLVLIENEPYHKVFTNMQSRIAKGILVGESKITPWAIPSGSIYPPMKNVMNASH
                     TIKHGAV"
BASE COUNT         4885 a         5050 c         4646 g         4505 t
ORIGIN      
        1 cctttggccc cgttctattt attggtgaac ttacaatctc accggatcga tatgccagat
       61 atgatcggcg tactctttga tagtacggtc agaagagaag tagcccatat tggcaatgtt
      121 cagcatcgct tttgcggtcc actcttcctg aagctcgtag agttcgtcga ctttatcctg
      181 acaatcgaca tagctgcgat aatccgccag aacctgatag tgatcgccga agttgatcag
      241 cgaatcaacc agatcgcgat agcgacccgg atcttccgga ctgaatacac cgctgccgat
      301 ttgcgtcagc acctgatgca gctcctcatc tttctcgtag tattcacgcg gtttgtagcc
      361 ctgacgacgc agttcttcca cttcttccgc tgtgttacca aaaataaaga tattgtcagc
      421 accgacatga tccagcatct cgacattcgc accgtccaac gtaccgatag tcagcgcacc
      481 gttaagcgca aacttcatgt tactggtgcc ggaagcttcc gtccctgcca gcgaaatctg
      541 ttcagacaga tctgccgccg gaatgatcaa ctgcgccagg ctaacgctgt agttcgggat
      601 gaacacgact ttcagcttat cgccaatctg cggatcgttg ttgatcactt tcgctacgtc
      661 attgatcaaa tgaataatgt gcttcgccat gtaataggcc gaagccgcct taccgccaaa
      721 aatattcacg cgcggtaccc acttcgcatc cgggtcggcc ttgatgcggt tatagcgggt
      781 aatcacatgc aacacattca tcaattgacg tttgtattcg tgaatacgtt tgatttgtac
      841 atcgaacaac gcctttggat tcaccaccac attcagctgc tgggcgatat actctgccag
      901 acgctttttg ttctccagct tcgcctgatg cacagcgtga ttaaccattg ggaaatcaca
      961 gtgttgttgc agctcattaa gcaggctaag gtcggtgcgc cagttacggc ccaggtgttc
     1021 gtccagcacg gctgaaagcg atgggttcgc taccgctagc cagcgacgcg gcgtcacacc
     1081 gttggtgacg ttggtgaaac gacccgggaa gattttcgca aagtcggcaa acaacgattg
     1141 caccatcaga ttagagtgca gttccgatac accgttaact ttgtggctca caacaaccgc
     1201 cagccaggcc atacgcacac gacgaccgtt ggattcatca atgatcgacg cccgtcccag
     1261 cagatcggta tcgttcggat actgttcctg caaggttttc aggaaatagt cgttgatttc
     1321 aaagatgatc tgcaggtgac gcggcagaat tttacccagc atatcaaccg gccaggtttc
     1381 cagcgcctcg ctcatcagcg tgtggttagt gtaggagaag acctgacaac acacctcaaa
     1441 cgcgtcgtcc cagctaaatt ggtgctcatc gatcagcaga cgcatcatct caggaatcga
     1501 cagtaccgga tgggtatcat tgagatgaat cgcgatttta tccgccaggt tatcgtaggt
     1561 tttatgcaac tgataatggc ggcttaaaat gtcctgaatg gtcgaggaaa ccagaaagta
     1621 ttcctgacgc aggcgcagct cacgcccgga gtaggtggag tcatccggat acagtacgcg
     1681 agatacgttc tcggagtggt ttttatcttc cactgccgcg aagtagtcac cttggttgaa
     1741 tttaccgagg ttaatttcgc tactggcttg cgcactccac aaacgcaacg tgttggtcgc
     1801 gtcggtgtcg taaccaggga ttatctgatc gtaagcgact cccagaatct cttcggtttc
     1861 aatccagcgc gtttttttac cttcctgctg aatgcgacca ccaaaacgga ctttatagcg
     1921 cgtgttgtgg cgtttgaatt cccacgggtt accgtattcc agccagtagt ctggcgactc
     1981 tttctggcta ccgttaacga tgttctgctt gaacataccg tagtcatagc ggatgccgta
     2041 accgcgcccc ggcaacccta acgtcgccag agaatcaagg aagcaagccg ccagacgtcc
     2101 caggccaccg ttaccgaggc ctgggtcatt ttcttcatca atcagctctt cgagatttaa
     2161 ccccatcgct tccagtgcgc cctgtacatc ttcgtaaatt cctagcgaca acatggcgtt
     2221 ggagagcgta cggccaatca aaaactccat cgacaggtag taaacctgac gagtttcttg
     2281 cgacaactgg gcacggtttg aacgtaacca gcgctccacg agacgatcgc gcacagcaaa
     2341 taacgttgcg ttcagccatt catgtttatt ggcgacgacc gggtcctttc caatcgtaaa
     2401 catcagcttg taagcgatag agtgcttaag agcttctacg ctaagcgtgg gcgatgaata
     2461 tgtaaacgga gcattcatat aggcgtttcc tgaaaactat ttcaagcgat agtaaagctc
     2521 acggtacgac ttcgccgcga cctgccagct aaaatccatt gccatagcct gacgttgcac
     2581 aaaccgccac agtgaagcac gagaccacag tacaaaagca cgtcgaatag cccgtaacag
     2641 cgaccaggca ttactatctt caaagacaaa cccactggcg acgccatctg caaggttctc
     2701 aagagaacag tcagaaaccg tatcagcaag cccaccggtg cgccgcacta acggcagcgt
     2761 accgtacttc aatccataaa gttgcgttaa gccgcacggt tcaaaacggc tgggcaccag
     2821 aatgacgtcc gcgccgccca taatgcgatg cgaaaatgct tcgtgatagc caatctgaac
     2881 gcccacctgg ccggggtatt ccgctgccgc cgcaaggaaa ccttcctgca gcaccggatc
     2941 gcccgcgccg agtagcgcca gctgcccgcc ctgctccaga agacccggta aggcttccag
     3001 caccaggtcg agacctttct ggctggtcag acggctcacc actgcaaaaa gcggcacttt
     3061 atcgtcaacc ttaagcccca ttgcgatttg taactggcgc ttattttccg ctttatcttc
     3121 caacgtatcg cgggtgtaac gcgaggccaa cagtaagtcc gtctctggac tccagatttt
     3181 ctcgtccacg ccgttcagta cgccggaaag acgcccttca cggtgacgct gttgcaacag
     3241 accttccata ccgtaggcaa actgcggttc ggtgatctcg cgagcgtagg ttggactgac
     3301 cgccgtaatg tgatcggcat agtacagacc ggccttcagg aaagagattt gtccgttgaa
     3361 ttccagccca tgaatattaa agaatgacca tggcaattgg atgtcattca tgtgatgtgc
     3421 ataaaacatg ccttgatagg ccaagttgtg cacagtaaac accgacttcg ccggacgccc
     3481 gcgcgccgcc agatacgcag gcgcaaggcc tgcatgccag tcgtgcgcat gcaccacatc
     3541 aggacgccag aatgggtcaa gcccgctggc catttctgcc ccaacccacc ccagcagcgc
     3601 aaaacgcaat acgttgtcgg tataggcaaa taagttggta tcgtgatacg ggcttcccgg
     3661 acgatcatag agatgcggcg cgtcaatcag gtatatgcca accccgttgt aatgaccgaa
     3721 caacagcgtg atatgtccgg cgaaggtatc acgacgggat actacctgcg catcggtcac
     3781 gccacggcga atatcgggaa atgcaggcaa cagtacgcga gcgtcaacgc cgtctgcgat
     3841 ttgtgctgcg ggtaatgccc caataacatc agccagaccg ccggttttaa gcagcgggaa
     3901 catctctgaa catacatgta aaacttgcat tatcgctcct gtttatgccc taacttccgt
     3961 agcatttcgc gcgttaccag cacgatgcct tcttctgaac gatagaaacg acgtgcatct
     4021 tcctctgcgt tttcaccaat caccatgcct tccgggataa cacaagcacg atcgatgacg
     4081 cagcggcgca gacggcacga gcgacctacc catacttccg gtaacaatac ggcggaatca
     4141 atgttgcaga atgaattcac gcgaacgcgc gagaacagaa cggactgcac caccaccgaa
     4201 ccggagatca cacaaccgcc ggataccagt gagttaaggg tcatcccgtg gctaccggag
     4261 cgatcctgca cgaatttcgc tggcggtaat gattcattgt aggtgcgaat tggccaattg
     4321 cgatcgtaca tatccagttc cggcaccaca gaggccagat cgagattcgc tttccagtaa
     4381 gctttcagcg tacccacatc gcgccagtac ggctcggcat ccgggtcgga ttgtacgcaa
     4441 gagagcggga acgggtgcgc ataggccaga ccggcttcgg tgatcttggg aatcaaatct
     4501 ttgccaaagt cgtggctgga gttctcatca cgatcgtctt cttccagcag ttcatacaga
     4561 tagtcggcgt caaagacgta gatacccata ctcgccagag atttgctcgg atcgttcggc
     4621 attgacggtg ggttagcagg tttttcaacg aattcgataa ttttatcgtt ctcatcaacc
     4681 gccataacgc caaatgcgga ggcttcttca atcggtactg gcatacaagc aacggtgcaa
     4741 cgtgcgcctt tttcgacgtg atcgataagc atacgcgagt agtcttgctt gtagatatgg
     4801 tcgcccgcca ggatcaccac gtattccgct ttataacggc ggataatgtc gaggttttgg
     4861 gtgaccgcat ctgcggtgcc gcgataccag ttttcccctt tcattctctg ctgtgctggc
     4921 agcagatcga caaactcgtt catttcttca ttgaagaatg accagccgcg ctgaatgtgc
     4981 tgcaccagag tgtgggactg gtactgggtg atcacgccca tacgacggat cccggagttg
     5041 atgcagttag acagcgcaaa gtcgataatg cggaacttac cgccgaagtg tacggccggt
     5101 tttgctcgct tattggttaa atccttcagg cgggtaccac gtcctcccgc cagtatcagg
     5161 gcaacagatt tcaatggcag ctggcgcgcc aacattaagt gatcgttctt ctctaaacta
     5221 accatgacta actccttttt tatcatctct ggaacacaca caatccgtgt gcaggtccct
     5281 gccagacagc cgtaatcact gggttatcct ctccagcgaa tgggggaatg gcgtgccact
     5341 ccccagcagg taaaacaatc tctgttacct caagcgtggc gttaattgcg atcaaaaagc
     5401 gatccgagag cagaatttgc agctgtttcg gcccgttttg ccactcatcc gtgcttaaag
     5461 gttgagcata tcgatttagc caacggacat tgccatcgcc ttcttcccac cagcgattct
     5521 ccaccaaagc gggaatgcgc ttgcgcagat ggattaacgc ggcggtaaat gcggttaaac
     5581 cactgcttgc ctgcgaccag tccaaccagg ttaattggtt atcctgacag taggcattgt
     5641 tattgccatg ctggctgtga ccatgttcgt caccggccag taacatcggc gtaccctggg
     5701 agagcaacaa cgttgttaac aaggcgtgaa tactgtcgcg ccgccgttca accaggtcaa
     5761 gagaaccgcc taacccttct ttaccatgat tgttactgta attgttgttg gtcccgtcgc
     5821 gattttcttc tccgtttgct tcattgtgtt tatggttgaa gcaaacgcag tcgcgaagcg
     5881 taaaaccgtc atgcgcagtg acgagattaa tcgcggcact cggcagacga ccattacgtt
     5941 taaaaacatc gctggaggca gcaaaacgcc cggcaaacgc ccccagaggc aaatcataat
     6001 gtagccagaa acgacgggca gcatcgcgga aatgatcgtt ccactcggca aacagcggcg
     6061 ggaaatttcc cacctgataa ccaccagggg cgatatccca cggttcagca attaacttca
     6121 cctgcgagag caccgggcag ttctggatag cggtaaacaa cggcgcatcc tgacggaact
     6181 ctggcgtacg gcccatgact gccgccagat caaagcggaa accatcgacg tgacaggttt
     6241 ctacccaata acgcaggcag gcgctggcat aatccaccac cgccggatga ctcaaattga
     6301 gcgtgttgcc gcaaccggtc cagttgtgat aatcgccgtc ttctcttatc caataatagc
     6361 tacggttatc gatcccacgc agtgagaata acgggccgtc gaggtccagt tccgcactat
     6421 ggttgagcac gatatcaaga atgacttcga tacccgcttt atgcagtgct ttgattgcat
     6481 cgcgaaactc atccagcgcc gtttctggcg agcaggcata cgccggatgc agcgcaaaca
     6541 tcgccaccgg gttgtaaccc cagtagttac ttagccccat gcgttgcaga cgtggttcac
     6601 tggcaaactg cgccactggc agcagttcca gcgcggtaat gcccaattgt ttcaaatagt
     6661 tgatcatcac cggatgcccg agggctttat aagtgccacg gatctcgacc ggaatctccg
     6721 ggtgcaagta cgttaatcct ttgacatggg cttcataaat gatggtgctg ccccacggcg
     6781 tgcgcggcgg ggcatcatct tcccagtcat agtgatcaac caccactacg catttcggcg
     6841 caatggcggc gttgtcgcga tagtcaggtt cattatgacc ggcgtgcagc agcgggttat
     6901 ctttaaattc cccgtcaatt tgccgcgcgc aaggatcaat caacaacttc gccgggttaa
     6961 agcgatgccc ctcggcgggt tgccaggggc catgaacgcg ataaccataa cgcaaacccg
     7021 ggcgcgcatc cggcagataa ccgtgccaaa tgtcgccact gtgccctggc aagtcatagc
     7081 gatgttcctg gccattggcg tcaaagacac acagttctac ccgctcggca tgagcggaga
     7141 aaagtgtgaa gttgacgccc tgaccgtcgt aatgcgcgcc gaggggagcg ggtttgccaa
     7201 tggcgagttg tgtcattctg cctcccgaac cagccagata gtggccagcg gtggtagtgt
     7261 caggcttagt gaatgctgac gaccgtggct ggcaatctca tcgctgtgta ccgtgccgcc
     7321 attgcctgca ttactgccgt gatagtgcat ggaatcggta ttgaggattt cacgccattt
     7381 gcctggctga ttaatgccga agcgataatc atgacgcggc accggcgtaa agttactggc
     7441 aacgataatt tcgttaccct ctttatcgcg acgcacaaag atcagcaccg agcgttcttt
     7501 gtcatccacc accagccatt caaagccgta cgggtcaaaa tccagttcat gcattgcttt
     7561 atggtggcgg taggtgaggt tcagatcgcg caccagacgc tggacaccgt ggtgccagtt
     7621 atcgccacct tccaacagat gccagtcgag gctggcgtca tggttccact cacggccctg
     7681 ggcaaattcg ttccccatga acagcagttt cttgcccggg aatgcccaca tccagccata
     7741 gtaggcgcgc aggttagcga atttctgcca tgcgtcgccc ggcatgcggt cgagaatcga
     7801 ttttttaccg tggaccactt catcatgcga caacggcagg acgaagtttt cagtgtagtt
     7861 gtagagaatc ccgaaggtga gtttatcgtg atgatactga cgataaaccg ggtcgagctt
     7921 catgtagtcc agggtgtcat gcatccagcc gaggttccac ttgtaccaga agcccagacc
     7981 gcccatatcc tgcggacgag aaacgccagg gaaatcggta gactcctcag ccattgtcac
     8041 cgcaccggaa acctgctcac caagaatacg gttggtatta cgcaagaatt caatcgcttc
     8101 aagattctcg cgcccgccaa attcgttcgg gatccactcc ccctctttac ggctgtagtc
     8161 gcgataaatc attgacgcca ccgcatcgac gcgcagcgca tcaataccaa aacgttcaat
     8221 ccagtaaagc gcgttaccga cgaggaagtt actgacttca cggcgaccat aattgtagat
     8281 cagcgtattc cagtcctgat gatagccttc gcgcggatcg ctgtgttcat acaagttcgt
     8341 gccatcaaat tcggcaagcg caaagtcatc ggtcgggaag tggcctggca cccagtcgag
     8401 aatcacgttc agacccgctg cgtgtgcggc atcaatgaaa taacggaagt cgtcgcgagt
     8461 accaaaacgg cgggttggtg catacaggcc ggttggctga taaccccaac tgccatcgaa
     8521 gggatgctcg ttaatgggca gtagttcgag gtgggtaaag cccatccatt tagcataagg
     8581 caccagttga tcggccagct cgcggtagct caaccagaaa ttgttatcgg tgtggcgacg
     8641 ccaggagccc aggtgaactt cataaataga gattgacgca tcaaactgat tcgctttttt
     8701 gcgctcttca gtctgtacaa ccttttccgg cagcccgcaa ataagagacg cggtttccgg
     8761 gcgcatttgc gcttcgaagg cataagggtc ggacttcaga cgcaagttgc cattggcatc
     8821 aatcatctcg tatttataga gctgaccgtt atgcgcccca gggataaaca gttcccaaat
     8881 gccgctctct ttacgcagac gcatcgggtg acggcgaccg tcccagtagt tgaattgccc
     8941 aaccaccgag acccgacggg cgtttggagc ccaaacagag aaacgcgtac ctgtgacgcc
     9001 atccatagta tctgcatgcg cgcctaaggt ttcatacggg cgcaggtgag taccttcaga
     9061 taatagccag gcatccattt cctggattag cgggccaaaa cggtaaggat catcaatcag
     9121 gttttgctga ccatgccaga caacagccaa ctgatagcgg aaaaaattct tacgtcgcgg
     9181 aatgacaccg ctaaagaatc cccgtgagtc aagacactcc agttttgcga gtttgcgccc
     9241 ggttttcggt tcaatcaccc acacatcggt agcgtcgggt aaaagggcac ggacttccag
     9301 tcccgcggtg gttttatgca ttcccagtac ggaaaaagga tccgcaaaat ggcctgcaat
     9361 tagcgcgtta atcacgtctc tatcgatacg atcggacatg cttgtcttcc tgttttattg
     9421 tgtcacccca tccaattgga tttttgactt ctggttgtga catttttttg acctgaacgg
     9481 cgcagcactc tgtgcatcct ctctgcgtcg tcctcacttc aggtaaggct gtgaatactc
     9541 atgtattcag ccacccttaa agaatagcca atgctctatt taactcccgg taaatcatga
     9601 aacatctgcg cttactcctg tattacgcac taacaggggc ggcatcgcgc cccagattta
     9661 atgaataaag attacgccag ttgacgaagc atccgacgca gcggctccgc ggccccccac
     9721 agcagctggt cacccacggt aaaggctgac aggaactccg gtcccatatt cagcttacgc
     9781 aggcggccta ccggcgtggt cagcgtgccg gtaacggcag ctggggttag ctcacgcata
     9841 gtgatttccc gatcgttcgg aacgactttc gcccacggat tgtgcgcagc cagcagttct
     9901 tcgacggtcg gaatagacac atcttttttc aatttaatag tgaatgcctg gctgtggcag
     9961 cgcaatgccc cgacacgcac acataaacca tctaccggaa ttacggaaga tgtgttgagg
    10021 atcttgttgg tttccgcctg ccctttccac tcttcgcggc tctgaccgtt atcgagctgt
    10081 ttgtcgatcc acggaatcag gctacccgcc agcggcacgc caaagttatc caccggcagc
    10141 tcaccgctac gggttaaggt tgtgactttg cgttcgatat cgagaatagc agaggacggg
    10201 gtcgcgagtt catctgccac atggccatac agatggccca tctgggttaa taactcacgc
    10261 atatgtcgcg caccaccgcc ggaagcggcc tggtaggttg caacggacac ccaatcaaca
    10321 agatcattgg cgaataaacc acccaacgac atcaacatca ggcttacggt acagttaccg
    10381 ccaacaaaag tcctgatgcc attatttaat ccgtcggtaa tgacgtcctg attgacgggg
    10441 tcaagaatga tgatggcgtc atctttcatg cgcagagacg atgctgcgtc aatccagtaa
    10501 ccttgccatc cgctttcacg aagctttgga tagatttcgt tggtataatc gccgccctga
    10561 caggtcacaa tgatatcgag ggcctttagc gcctccagat caaaggcatc ctgaagtgtg
    10621 ccagtggttc cgccaaaaga cggcgcagcc tggccaagct gagaagtaga aaagaagaca
    10681 gggcgaatgg cgtcgaagtc gcgctcttca accatgcgtt gcatgagaac ggagccgacc
    10741 ataccgcgcc agccgataaa accaacattt ttcataagcg ttttttcctg caaagatgtg
    10801 tgctgtataa atgtgccggt ctcctcttgg cacatctttc accatacaaa aagcagccaa
    10861 agtcgcaagt gaaattaatc aatgatagcg aagccatcag taatgcgact tatcctgctt
    10921 tgttagcaca cagaaagtcc gcggcaatta tcagggaatt tgagttatga atgaaatcat
    10981 ttctgcagca gttttattga tcctgattat ggatccgctc ggaaacctgc ctattttcat
    11041 gtccgtactg aaacatactg aaccgaaaag acggcgggca atcatggtgc gagagttgct
    11101 tattgctctc ctggtgatgc tggtgttcct gtttgcgggt gagaaaattc tggcatttct
    11161 cagcctgcga gcagaaaccg tctccatttc tggcggcatc attctgtttc taatcgccat
    11221 taagatgatt ttccccagcg cttcaggaaa tagcagcggg cttccggcag gtgaagagcc
    11281 atttatcgtg ccgttggcaa ttccgttagt cgccgggccg actattctcg ccacgctgat
    11341 gttgttgtct catcagtacc cgaatcagat gggacatctg gtgattgctc tgctgctggc
    11401 ctggggcggc acctttgtca tcctgctaca gtcttcgcta tttttacgtc tgctgggcga
    11461 gaaaggggtg aacgcacttg aacgcctgat gggattgatt ctggtgatga tggcaaccca
    11521 gatgttcctc gacggcattc gaatgtggat gaaggggtaa cgaggagagt ttttcgaaaa
    11581 gaacccggta gcgtcacaac taccgggcga acaaacttaa ctcaacagct ggaacgcaat
    11641 catcccaacg atggcaccaa cagtgccgag gatggtttcc atcatggtcc aggttttcag
    11701 cgtttcggct tcggtcgcgc cggtaaattt accgaacaac cagaaaccgg cgtcgttaac
    11761 gtggctgaca acaatcgaac caccagcgat acaaatcgac agtgccgcca tttgcgcacc
    11821 ggagtagttc agttgttcaa tgaccggcat caccagcccc accgccgtta aacaggcaac
    11881 ggtggcagac ccctgaatga tgcgcactgc agcggccagc acgaagcagg tgatagcaat
    11941 cggcaggccc atgccggtta acgcttcgcc cagtgccgga cctacgccag agtcaaccag
    12001 cacctgtttg aacacgccgc ctgcaccaat caccagcaga atgatccccg ccggttgtag
    12061 cgcgtgaccg caaatctcca tcactttgtc ttttggcatg ccctgacgca ttgccaggcc
    12121 ataaatcgct accagacaag caaccagaat cgcggtaaat ggatgaccaa taaactcgaa
    12181 ccattcgtaa gcagttgatc cttctggcac aaaacgagcg gcaatggttt tcagccccac
    12241 cagcaccagc ggcaacagga tcagcgacag gctgaatccg aaggacggca ttttgccttc
    12301 gccgagatgc ggttcgctga tgtcgtcagg aatatgcaac tccacgtaac ggctgatgaa
    12361 attaccccac agcggcccgg caataatcat tcccggaatt gccgcacaca gaccaatcag
    12421 gatcatccag ccaaagtcgg cattcatctg cgatgccagc aacatcggcg ctggtccagg
    12481 caccagaaat gccgccgctg ccgccacgcc tgcgaataat gggattacca gcttcaccag
    12541 gttcgtaccg gtgtggcgcg ccattgagaa agcaacgcta atcagcagaa ctatcgccac
    12601 ttcaaagaac agcggtagcg cacagaccaa ccccgcaagg ccgatggcat aatgcgcgcg
    12661 gctgtgaccg aaggatttga gcattttgac ggcaatctga tcgactgcgc cggtttcatg
    12721 taagatcttg ccaaacatag ctcccagggc gacaaccacc gccaggaagc cgagggggcc
    12781 tcccatccct ttttccatcg tcgctgcgat tttatcgagc ggcataccgg aaaaaaggcc
    12841 agcccccatg gacaccacca tcaaagccag gaaagcgtgc atacgcgcct tcatgactaa
    12901 aaacagcagc agtaaaacag accctactgc tgttaaaaca agcgttaatg tagtcactac
    12961 ttatttgcct tttttaataa cctcaatggt gcttgccaca acaccttcca gcggttgatc
    13021 gatatccacc accagtacat cggtttcgtc cgcacccggc tcctgcagcg tttcaaactg
    13081 cgtcaccaac atttgggttt taaagaaatg gcctttgcgc gctttcaagc gactttcaat
    13141 cacatcaaaa tcgcctttca ggtagatgaa agagagattc ggattacctt cacgcagcaa
    13201 gtcgcgatag tgctttttca gtgcagaaca gacgatcagc gacactttat tggtgcgcag
    13261 catagcaaac gcggcgtcgt tcagcgcctg caaccagggt ttgcgatcgt cgtcattcag
    13321 cggttcgcca gacgccattt tttcgatatt gcgccgtgga tggaggaaat caccatcaag
    13381 aaacgcggca tgaagttgat gcgccacttc actggcgacc gcagatttgc cgctgcccga
    13441 tacgcccatc aagacgtaaa tgtggtgatc atggttagtc gtgctcaaag tggtgccccc
    13501 acaatacaag aattaaaaat gttacgggta actgttatcg gtaacattgt ccagccggac
    13561 aatatcagaa gcaatatcca tccgtgcctt aagtgtataa gtgtgagcta cttcaaattt
    13621 gtaggcttaa atagatccgc ccggtgacaa ggtgaaacct aaatctaaca ttttcggtgt
    13681 cacagattcg ccacgaatac gcgccagcag gcgttcagcg ccaatactgc ccatccgctc
    13741 acgcggcgtc agcacgctcg ctaaccgcgg ctccatcacc tggccaatgt catgaccgtg
    13801 gaaaccggca atcgccatat cgtcaggaac cttcaatccc agacgctgac attcaaacgc
    13861 cgcgccgacc gccaggtcat cgttggtaca gaacacgcca tccagctgcg gatattcccg
    13921 ccgcgcctgg cgaatcagtt caataccgga agagtaagaa gaagattgct caaccatcac
    13981 gctatacgac accagacctg catccagcat cgcctgttcg tatcccttct gtttgatgat
    14041 agtacgttcg tcgagacgcg cgccgagata ggcaatgtgg caatgcccgc gagcaataat
    14101 ggcggtggtc atctggcgtg ccgcttcaaa gttatcaaaa ccgacggcga tatcgaggca
    14161 cggcgactgg ctgtccatga gttccaccac cgggatcccc gccacttcaa tcatctttaa
    14221 ggtgcgcggc gtgtgggtac gttcggtgag gatcagtccg tcgatattcc atgagagcat
    14281 ggattcgaga cgttcttgct ccatttccgg tttataaccg tagtgagcca gcatggtctg
    14341 ataaccgtgc gcgtcggtga cgctttcgat tccgcgtaat acttccgcga aaacctggtt
    14401 ggtgagagaa ggtaacagga cgccaatcgc ccggctggtg gcgttagaga ggatatcggg
    14461 cgcacgattg ggaatatagc ccagttcatc aagagcggcg gcaatcttgc cgcgtagagc
    14521 gacggaaacc tgctccgggt tgcgtaaaaa acggctgacc gtcattttgg tcacgcctac
    14581 acggtcagcc acatcctgaa gtacgggtct tttctttttc atcgtcctga aggtacaaaa
    14641 gagatagatt ctcttagttt aacacggacg catcagaacc ttcccgggga aaacagtgct
    14701 tgtttacaat tattagatat acactaaatc aattgcatcg cattgtgcta acgacgcggc
    14761 aaaaccccgg aagcgtgcat cagcacgcga ccggagcgag cgaggaagcc tgcaaaaagg
    14821 caggcttcaa agatgccgtt gagttttaaa ccggcggcag atcgaacagt aacacttcgc
    14881 tgtcgctatc cgcatggatg gagattgcct gctcatccca gattgccaga ccatcgctgg
    14941 tcgaggcttt cacgccgtta atggtgacat tgcctttcac cacctggatc cagacgcggc
    15001 gttcagcggc aatctgatgc accgactgct catctttcag caacgcccag cggtacagtt
    15061 ccatatcctg atgcactttc aatgaaccat ctcgcgcatc cggcgagagc accagctgtt
    15121 tgccctgtac ggcatcgaag cgacgctgtt cataacgcgg cgtaataccg ttttcttcag
    15181 gcatgatcca gatctgatac agatgcagac gctcggtgct gcttgggttg tactctgagt
    15241 gacgaatacc cgtacccgca ctcataatct ggaactcacc cgccggaacc tgctctttat
    15301 tgcccatgct gtcctgatgc tcaacagtac cttccagcac gtaggtcaaa atttccatat
    15361 ctttatgcgg gtgagtgccg aagccctgcc ctgcttcaat cacgtcgtcg ttaatcacgc
    15421 gcagcgcgga gaagcccata aagttcggat cgtaatagtt ggcaaaagag aaagtatgcc
    15481 aggagtccag ccagccatga tttgcatgac cacgttcatt tgctttgcgt aagtagatca
    15541 tttgtattcg ccccctgaat gatttcgatg ggcttagtgt ggacccgatc tgcctgggat
    15601 gatagagggt gaaaattgcc ccctctgttc aaaaaaattg aactattcga ggggccatca
    15661 aacttaattc gcgagagtca ctgtggaggg agaggcttgc tcaaatccgc gttcaaggat
    15721 ttccagattg gtaagaactt cagattcctt gacgtaattt ggcgcaccgt gggtgatggt
    15781 ttgatacaac gcatcataaa cgcgcccgta atcgcccatc tccggcttca tctcttctct
    15841 gaccgtcacg ccctcgtcat tgacatactc cggcacaccg accgaatcat ccgctgcgaa
    15901 tcccggttcg cccggcataa tattagcctt caggctggtt tcctgctggt cgataccgta
    15961 tttaataaac gaacctttct taccgtgaac gataaatttc ggataatcga ttttcaccag
    16021 atggctggtt ttgacgatgg ctttcaggtc gccataaaac agttgcgctt caaaggtgtc
    16081 gtcaggattg gctttattac gcaggctgcg gatgtcataa gcgacgtgat ccgggcgacc
    16141 gaacagagaa ataatctggt ccatcgtatg cacaccaagg ccataaaacg cgccatcctg
    16201 cggcagccca ggtttggttt ctgccaccgg gcggtaatag tcaaaatggc tttccacttc
    16261 aacaatctct cccaacttgc cactttcaat cgcttttttc gctgtcagga agcaggagtc
    16321 aaagcgacga ttctgatacg gcgtgacgct cagccctttg cttttcgcca acgcaaacag
    16381 ctccttcgcc tgggcaagcg tcggggtgaa cggtttttcg accagcacat ttttcccggc
    16441 ttccagcgcg cgtttcgcgt actcgaaatg gctgtccgcg tgggtgcaga caacaaccag
    16501 cttaacatcg ggatcgttta gtacttcgtc gagatcgctg atgaaatgga tatgggaata
    16561 aatgggagcc tgttcttccg gcttcgcatg gcgacgaaaa atatgcgcga catgccagct
    16621 atccttgcgg ttaagtacat acggcagatg gtaacgggtg gtgcttttgc cgaagccaat
    16681 aaaggcgcag ttgatgacca tgatttcgtc ctttttaagg tggttattta caccttagcg
    16741 caaagcggac gtggttccta ctggagtgcg cataacgaac acaagcactc ccgtggataa
    16801 attgagaacg aaagatcaaa aaaaagccag cacccggctg gctaagtaat actggaagca
    16861 atgtgagcaa tgtcgtgctt tcaggttctc cgcgagggtc ttcctgatcg cgagacaata
    16921 ataatcattc tcattcgcgc ttgtccaaca ctttttgcaa aaaaatgcat ttgactcgca
    16981 tttgaaagtc aatgatgttg aaagggacat ttaccccaaa gaggacaaag gaatgagtga
    17041 gatagtaata cgccacgcag aaacacggga ttacgaggcc atcaggcaga ttcacgccca
    17101 gccggaggtg tattgcaaca cactacaggt gcctcatcct tccgatcata tgtggcagga
    17161 gcgactcgcc gatcgtcccg gcatcaagca actcgtcgcc tgtattgatg gagacgtcgt
    17221 gggccatctc accattgacg tgcaacagcg cccacgcagc agtcatgttg ccgattttgg
    17281 tatctgtgtc gactctcgtt ggaagaaccg cggcgtcgcc agcgccctga tgcgagagat
    17341 gattgaaatg tgcgacaact ggttgcgggt agatcgcatt gaactaaccg tgtttgtcga
    17401 taacgccccg gcaattaagg tctataaaaa atacggcttt gaaattgaag ggactggtaa
    17461 gaagtacgca ttgcgtaatg gtgaatatgt cgatgcatat tatatggcgc gggtgaagta
    17521 agatagtgcc ctttttctga gatggaaaaa gggtgtcatt caaaatcgac ataccttcct
    17581 ttaaggtatt tatttaccca atacatatat tgagatttaa ctcataatta attcctaaag
    17641 tgtaatattt tattttttaa tatatacgcc tacaatttcc tggagtaaat aaataacaat
    17701 taacaagcat aatattgcca ttgataaaat agcatgccat aaaaggactt ttcagggatg
    17761 agtaatattg tttacctgac agtaacggga gaacaacaag gaagcatctc cgcaggttgt
    17821 gggacttctg agtctacagg taatcgttgg cagagcgggc atgaggatga aatatttaca
    17881 ttctcactct taaataatat taataatacg gggcttggtt cacagttcca tggtataaca
    17941 ttttgtaaat taattgataa aagcactcca ttatttatta attccattaa caataatgaa
    18001 caattattta tgggatttga cttctatcga ataaatagat ttggtagatt ggaaaagtat
    18061 tattatatac aactaagagg cgctttttta tcggctattc atcaccagat cattgaaaac
    18121 caactggata cagaaacaat aactattagt tatgaattta tcctctgtca acatcttatc
    18181 gcaaataccg agttcagcta tttggcactc cctgaaaatt ataaccgatt gtttttacca
    18241 aattcaaaaa accaaacaaa taatcgtttc aaaacgttaa acagcaaagc tattggcagg
    18301 ctacttgctg ctggtggcgt atacaatggg aacattgaag gattcagaga tactgcggaa
    18361 aaactgggtg gagatgcaat aaaaggctat gatcaaatac taaatgaaaa aacagcgggc
    18421 atagcgatag caacagcatc tattctttta acaaagcgtt ctaatgttga tacatataca
    18481 gaaataaata gttacttagg caaacttaga ggtcaacaaa aacttcttga tggtatagac
    18541 ataatagaaa taatatacat taagagacct tcaaaagact tagctaactt acgaaaggag
    18601 tttaataaaa ctgtaagaaa aaattttctt atcaaacttt caaaaacctc cgaagcatct
    18661 ggaagattca acgccgaaga ccttttaaga atgagaaagg gcaatgttcc tctaaattat
    18721 aatgttcacc ataaactatc tctagatgat ggtggtacta atgatttcga aaatttagta
    18781 ttaatcgaaa acgaaccata tcataaagtt tttactaaca tgcaatcacg aatagctaag
    18841 ggaatattag taggtgaaag caaaatcact ccctgggcca ttccatctgg ctcaatttat
    18901 cctcccatga aaaatgtaat gaacgcatcc cacacaatta aacacggagc agtatgatgg
    18961 ttaaaagggt gaaactggca ggtgtggagc ctcgcagatt tccggcatca tagtgcccaa
    19021 tacgggatgc ataaagctcc ctcagactgg aaactccaca aatgaaatat acaccggttg
    19081 gcgttg
//
LOCUS       DAJTMC010000077        18745 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.077, whole genome shotgun sequence.
ACCESSION   DAJTMC010000077 DAJTMC010000000
VERSION     DAJTMC010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18745
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.077"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            <1..893
                     /locus_tag="OO130_002319"
     CDS             <1..893
                     /locus_tag="OO130_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5365707.1"
                     /translation="WFNIKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFIT
                     IGVLGIFLAIGWYMLYRNREHVELTAVEQAYLNAGSVNARRDPLSFAEWRSLFRNRTM
                     WGMMLGFSGINYTAWLYLAWLPGYLQTAYNLDLKSTGLMTAIPFLFGAAGMLVNGYVT
                     DWLVKGGMAPIKSRKICIIAGMFCSAAFTLIVPQATTSMTAVLLIGMALFCIHFAGTS
                     CRGLIHVAVASRMTASVGSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTA
                     AGALAYIFLVRQPINDPRKD"
     gene            complement(942..2597)
                     /gene="tsr"
                     /locus_tag="OO130_002320"
     CDS             complement(942..2597)
                     /gene="tsr"
                     /locus_tag="OO130_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HCR5365708.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGIAFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQRETSAVVKTVTPAAPRKMAVADSEENWETF"
     gene            complement(2716..3162)
                     /gene="hpaR"
                     /locus_tag="OO130_002321"
     CDS             complement(2716..3162)
                     /gene="hpaR"
                     /locus_tag="OO130_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HCR5365709.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEAIPGDNE"
     gene            3434..4723
                     /locus_tag="OO130_002322"
     CDS             3434..4723
                     /locus_tag="OO130_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HCR5365710.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPGDRVRVLAEGFPPLENPVVDEREVTTRKSFPTQ
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEDTAK"
     gene            4720..6186
                     /gene="hpaE"
                     /locus_tag="OO130_002323"
     CDS             4720..6186
                     /gene="hpaE"
                     /locus_tag="OO130_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HCR5365711.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWANLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALVSPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADRLGELALEAGIPAGVLNVVQGYGATAGDALVRDHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVGALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            6188..7039
                     /gene="hpaD"
                     /locus_tag="OO130_002324"
     CDS             6188..7039
                     /gene="hpaD"
                     /locus_tag="OO130_002324"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HCR5365712.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     ADEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            7049..7429
                     /locus_tag="OO130_002325"
     CDS             7049..7429
                     /locus_tag="OO130_002325"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HCR5365713.1"
                     /translation="MPHFIVECSDNIREEADLPGLFAKVNPTLAATGIFPLAGIRSRV
                     HWVDTWQMADGQRDYAFVHMTLKIGAGRSLESRQQAGEMLFELIKTHFAALMESRLLA
                     LSFEIEELHPTLNFKQNNVHALFK"
     gene            7540..8343
                     /gene="hpaH"
                     /locus_tag="OO130_002326"
     CDS             7540..8343
                     /gene="hpaH"
                     /locus_tag="OO130_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HCR5365714.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAEGRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSISCRFV"
     gene            8354..9142
                     /gene="hpaI"
                     /locus_tag="OO130_002327"
     CDS             8354..9142
                     /gene="hpaI"
                     /locus_tag="OO130_002327"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HCR5365715.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGAGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     EAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIRESGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGAQATAVKPGVY"
     gene            9317..10693
                     /gene="hpaX"
                     /locus_tag="OO130_002328"
     CDS             9317..10693
                     /gene="hpaX"
                     /locus_tag="OO130_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HCR5365716.1"
                     /translation="MSDTSPAIPESIDPANQHKALTAGQQAVIKKLFRRLIVFLFVLF
                     IFSFLDRINIGFAGLTMGRDLGLSATMFGLATTLFYAAYVIFGIPSNIMLSIVGARRW
                     IATIMVLWGIASTATMFATGPTSLYVLRILVGITEAGFLPGILLYLTFWFPAYFRARA
                     NALFMVAMPVTTALGSIVSGYILSLDGVMALKGWQWLFLLEGFPSVLLGVMVWFWLDD
                     SPDKAKWLTKEDKKCLQEMMDNDRLTLVQPEGAISHHAMQQRSMWREIFTPVVMMYTL
                     AYFCLTNTLSAISIWTPQILQSFNQGSSNITIGLLAAVPQICTILGMVYWSRHSDRRQ
                     ERRHHTALPYLFAAAGWLLASATDHNMIQMLGIIMASTGSFSAMAIFWTTPDQSISLR
                     ARAIGIAVINATGNIGSALSPFMIGWLKDLTGSFNSGLWFVAALLVIGAGIIWAIPMQ
                     SSRPRATP"
     gene            10703..11589
                     /gene="hpaA"
                     /locus_tag="OO130_002329"
                     /pseudo
     CDS             10703..11589
                     /gene="hpaA"
                     /locus_tag="OO130_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224927.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
     gene            11840..13402
                     /gene="hpaB"
                     /locus_tag="OO130_002330"
     CDS             11840..13402
                     /gene="hpaB"
                     /locus_tag="OO130_002330"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
                     /protein_id="HCR5365717.1"
                     /translation="MKPEDFRASTQRPFTGEEYLKSLQDGREIYIYGERVKDVTTHPA
                     FRNAAASVAQLYDALHKPEMQDSLCWNTDTGSGGYTHKFFRVAKSANDLRQQRDAIAE
                     WSRLSYGWMGRTPDYKAAFGCALGANPGFYGQFEQNARNWYTRIQETGLYFNHAIVNP
                     PIDRHLPTDKVKDVYIKLEKETDAGIIVSGAKVVATNSALTHYNMIGFGSAQVMGENP
                     DFALMFVAPMDADGVKLISRASYEMVAGATGSPYDYPLSSRFDENDAILVMDNVLIPW
                     ENVLIYRDFDRCRRWTMEGGFARMYPLQACVRLAVKLDFITALLKKSLECTGTLEFRG
                     VQADLGEVVAWRNTFWALSDSMCSEATPWVNGAYLPDHAALQTYRVLAPMAYAKIKNI
                     IERNVTSGLIYLPSSARDLNNPQIDQYLAKYVRGSNGMDHVQHIKILKLMWDAIGSEF
                     GGRHELYEINYSGSQDEIRLQCLRQAQSSGNMDKMMAMVDRCLSEYDQNGWTVPHLHN
                     NDDINMLDKLLK"
     gene            13420..13932
                     /locus_tag="OO130_002331"
     CDS             13420..13932
                     /locus_tag="OO130_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HCR5365718.1"
                     /translation="MQLDEQRLRFRDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            14325..16475
                     /gene="btsT"
                     /locus_tag="OO130_002332"
     CDS             14325..16475
                     /gene="btsT"
                     /locus_tag="OO130_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HCR5365719.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            16606..16809
                     /locus_tag="OO130_002333"
     CDS             16606..16809
                     /locus_tag="OO130_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HCR5365720.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            16820..17776
                     /gene="yjiA"
                     /locus_tag="OO130_002334"
     CDS             16820..17776
                     /gene="yjiA"
                     /locus_tag="OO130_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HCR5365721.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEDEIRAAFAGLKK"
     gene            complement(17878..>18745)
                     /locus_tag="OO130_002335"
     CDS             complement(17878..>18745)
                     /locus_tag="OO130_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HCR5365722.1"
                     /translation="DRVTEIDRLLEQKPRLTADQAWDVIRQTSRQDLNLRLFLPTLQA
                     ATSGLTQSDPRRQLVDTLTRWDGINLLNDDGKTWQQPGSAILNVWLTSMLKRTVVAAV
                     PMPFDKWYSASGYETTQDGPTGSLNISVGAKILYEAVQGDKSPIPQAVDLFAGKPQQE
                     VVLAALEDTWETLSKRYGNNVSNWKTPAMALTFRANNFFDVPQAAAEETRHQAEYQNR
                     GTENDMIVFSPTTSDRPVLAWDVVAPGQSGFIAPDGTVDQHYEDQLKMYENFGRKSLW
                     LTKQDVEAHKES"
BASE COUNT         4289 a         5186 c         4992 g         4278 t
ORIGIN      
        1 actggttcaa catcaaagag cgcggacgcc cgatgggctt cttcaacgca gcttctacca
       61 ttggcgttgc cgtaagccca ccgattctgg cggcgatgat gctggtgatg ggctggcgcg
      121 ggatgtttat taccattggt gtactgggga tttttctcgc catcggctgg tatatgctct
      181 atcgcaaccg cgagcacgta gaactgactg ccgttgagca agcttatctc aatgcaggta
      241 gcgtcaatgc ccgccgagat ccgctcagtt ttgccgaatg gcgcagcctg ttccgtaacc
      301 gtacaatgtg gggaatgatg ctcggattca gtggcatcaa ctacactgcg tggctgtatc
      361 tggcctggct tcctggttac ctgcaaacag cctataacct ggatttaaaa agcacagggt
      421 tgatgacggc tatccctttc ctgtttgggg ctgccgggat gctggtcaac ggttacgtta
      481 ccgactggct ggtcaaaggg ggaatggctc cgattaaaag ccgtaagatc tgcattattg
      541 ccgggatgtt ctgttctgcc gcctttacgc tgatagtacc acaagcgaca acatccatga
      601 cggcggttct gctgattggc atggcactgt tctgtattca ctttgccgga acatcctgcc
      661 ggggcttgat ccacgtcgca gttgcttctc gcatgactgc gtcggtgggc agtatccaga
      721 actttgccag cttcatctgc gcctcttttg cgccgatcat tactggtttt attgttgata
      781 ccacccactc attccgtctg gcactaatca tctgcggttg cgtcaccgca gcgggggcac
      841 tggcgtacat cttcctggtt cgtcagccga tcaacgaccc acgtaaagat taatctcctt
      901 atgcccgata acattttgct tatcgggcat tttcatggcg attaaaatgt ttcccagttc
      961 tcctcgctat ctgccacggc cattttacgc ggcgcagctg gcgtcacggt ttttaccaca
     1021 gccgatgttt cacgctgctg ttgctgaatc cggaacactg ccactgcttc ggtcaggcga
     1081 ctggcctgct cttccagcgc ggcggcggca gcggcagact cttccaccag cgcggcgttc
     1141 tgttgagtta cccggtccat ctcagcaacc gctaagccaa cctgatcgat accacggctc
     1201 tgctcatcag aagcagaagc aatttcgccc ataatgtccg tcacgcgggt cacggcgctg
     1261 acaatctccg ccattgtttc cccggcgctt tcgaccagcg tagagccaac atccactttc
     1321 cccaccgagt cttcaatcag gcttttaatt tcacgagccg cctgggcgct gcgctgggcc
     1381 agattacgca cttctcccgc gaccaccgca aaaccgcgcc cttgctcacc cgcacgcgca
     1441 gcctcaaccg ccgcgttcaa agccagaata ttggtctgga aggcaatgcc gtcaattacg
     1501 ctgataatat cggcgatttt ctgcgaactg gtggagatat cgcgcatagt ctgcaccacg
     1561 ttatctacca ctttgccgcc gcgttgcgcc gtttcagaag cacttaacgc cagatggctg
     1621 gcctggcgcg cattttcggc gttctgcttc accgttgcgg tcagttgctc catgctggct
     1681 gccgtctctt ccagcgaagc ggcctgttgc tcggtgcgcg aagagagatc gttattgccg
     1741 gtggcgattt cgctggcacc gctatagatg gcattagccc cgttgcgcac atcaccgacg
     1801 gtacgcatca gctctccctg catatggcgc aaactctctg ccagttgccc catctcatta
     1861 gagccatcca cctcaatcgg tttcaccaga tcgccgcctg caatatgacg aatgctgtca
     1921 atcaggcgat tcattggcgc taccagcgag gctttaatac cgaaccagac ggcgaagatg
     1981 accgccagta cgacgatcat cacgcccacc agaatccaca tcgcctggct gtaggaggca
     2041 ttgttatcgc tgacggcgat atcatagagc cgatcgtttt gctccatgta agccacatac
     2101 tgcttctcga aaccgtcctg atatccctgg gtcggctgat caaagaactc gttgattttg
     2161 cctgcaccta acagttggat cagctccgcc agcgcattgt gataaatatc gtaattacgt
     2221 ttgatctctg ccgctgcggc ggtgctctga cgcgggtcac gcggcaacgc ttcgtaatcc
     2281 gcccagtttt tttccgcctg tttcagcgaa atactggcac tctgcatcag ctcagcaacg
     2341 gttgaaccgc taccaatatt attctgatcc atcatgtagc ggatacccgc gcggttgagg
     2401 gtgttacgcg tctgcaacaa cgcgacccag ctgccattca gcgtggattg ctgctggcga
     2461 atggtttgta aaacagtgaa attttctttg tcattcttta aggcattaaa gaacagaccg
     2521 cctgatgtca gttgtaaaag gccaaaaacg gccaaaacca gcagtaagct ggtcacgatc
     2581 ttgatacgtt ttaacatggt ttctctttcc tgtggaccga tgcaagattt tcggcctgga
     2641 aagcaaaaac tttatgaaaa ggtgtaaccc gccatccatg gaaaaaccag gcgctggtta
     2701 tcagatacta aaaagttatt cattatcgcc cgggatggct tcttgtcggg aattgcccag
     2761 agcaataaat tcctctaaca gatgggttaa ctgttgcatt ttctcggcag taaactgagc
     2821 ttcaatctgc cgataggctt cttcaatctg tgtctgagcg cggttataca gcgcctgccc
     2881 ctctttggtt agcgagatat agagcttccg ctgatcattg atcggcttta atcgcaacac
     2941 taaaccgtcg cgttccatcc gcgtgagaat cccggtcagg cttgggcgca aaatgcaggc
     3001 gcgatacgcc agatcgtgaa aatccattga tgggctttcc gccagaatac gcacgatgcg
     3061 ccactgttgc tcagtcaaat tatgccgttt aacgattggg cgaaaataac tcatcgccgc
     3121 ttctcgcgcc tggagcaacg caatggttag tgagtcgtgc attatctttc ccctgaaaaa
     3181 ttcaaaatca ttaatataga aacagttaat cgtcacgtta ccgggactgc gctgcccctg
     3241 caattcctga ttttatcaaa attgatgata ttttattaac ttactgatat ttaatatttt
     3301 attatcttta aatgtaaata gtttgttaat tagatcacat ttacatcact tacttttgcc
     3361 aaactacgca atcgggaata aaagcaatca ttaacatatt aatgattaag ataatcgaat
     3421 ccgaggagtg gtaatgaaag gcactatctt cgccgtagcg ttgaaccatc gcagccaact
     3481 tgatgcatgg caggaagcgt tccagcaatc cccctacaaa gccccgccta aaactgcggt
     3541 ctggtttatt aaaccgcgca atacggtgat tggttgcggt gaaccgattc cctttccaca
     3601 gggtgaaaag gtactgagcg gtgcgactgt tgcgctgatt gtgggaaaaa cggcgacgaa
     3661 agtacgtgaa gaagatgcgg cagagtacat cgccggatat gcgctggcta acgacgtcag
     3721 cctgccggaa gagagctttt accgcccggc aatcaaagca aaatgtcgtg atggattctg
     3781 ccccattggc gaaaccgtgg ctctcagcaa tgtcgataat ctgaccatct ataccgagat
     3841 caacgggcgt cctgccgatc actggaatac cgccgattta caacgtaacg ccgcgcagtt
     3901 gctgagcgcc ctgagcgaat ttgccacgct gaatccaggc gacgccattc tgctcggcac
     3961 gccacaggcg cgcgtggaaa tacagccagg cgatcgcgtt cgtgttctcg cagaaggttt
     4021 cccgccgctg gaaaatccgg tagtggacga acgtgaagtg accacgcgca agagcttccc
     4081 aacgcagcca cacccgcacg gcacgctgtt tgccctcggc ctgaactacg ccgaccacgc
     4141 cagcgaactg gaatttaagc caccggaaga accgctggta ttcctgaaag cgccaaatac
     4201 cctcactggc gataaccaga cctccgtgcg tccaaacaat attgaataca tgcactatga
     4261 agcggagctg gtggtagtta ttggcaaaca ggcgcgtaac gtcagcgaag ccgatgccat
     4321 ggattatgtc gcgggctaca ccgtgtgtaa cgactacgcc attcgtgact atctggaaaa
     4381 ctactaccgc cctaacctgc gggtaaaaag ccgcgacgga ctgacgccga tgctttcaac
     4441 catcgtgccg aaagaggcga tcccggaccc gcataatctg acccttcgca ccttcgtcaa
     4501 cggcgagtta cgccagcaag gcaccaccgc cgatctgatc ttcagcgtgc ccttcctgat
     4561 cgcctactta agtgaattta tgaccctgaa tccgggcgac atgatcgcca ccggcacacc
     4621 caaaggctta tctgacgtgg tgcctggcga tgaagtagtg gtggaagtag aaggcgtggg
     4681 ccgcctggtg aaccgaattg tgagtgagga tacagcgaaa tgaaaaaagt aaatcattgg
     4741 atcaacggca aaaatgttgc aggtaacgac tacttccaga ccaccaatcc ggcaacgggt
     4801 gaagtgctgg cggatgtggc ctctggcggt gaagcggaga tcaatcaggc ggtagcggca
     4861 gcgaaagagg cgttcccgaa atgggccaat ctgccgatga aagagcgtgc gcgcctgatg
     4921 cgccgtctgg gcgatctgat cgaccagaac gtgccagaga tcgccgcgat ggaaaccgcg
     4981 gacaccggcc tgccgatcca tcagaccaaa aatgtgttga tcccacgcgc ttcccacaac
     5041 tttgaatttt tcgcggaagt ctgccagcag atgaacggca agacctatcc ggttgacgac
     5101 aagatgctca actacacgct ggtgcagccg gtgggcgttt gtgcgctggt atcgccgtgg
     5161 aacgtaccgt ttatgaccgc cacatggaag gtcgcgccgt gtctggcgct gggcaatacc
     5221 gcggtactga aaatgtcgga actctccccg ctgaccgctg accgcctggg tgagctggcg
     5281 ctggaagccg gtattccggc aggcgtgctg aacgtggtac agggctacgg cgcaaccgca
     5341 ggggatgcgc tggttcgtga tcatgacgta cgtgccgtgt cgttcaccgg cggtacggcc
     5401 accgggcgca acatcatgaa aaacgccggg ctgaaaaaat actccatgga actgggcggt
     5461 aaatcgccgg tgctgatttt tgaagatgcc gatattgaac gcgcgctgga cgccgccctg
     5521 ttcaccatct tctcgatcaa cggcgaacgc tgcaccgccg gttcacgcat ctttattcag
     5581 caaagcatct acccggaatt cgttaaacgc tttgccgaac gcgccaaccg tctgcgcgtg
     5641 ggcgatccga acgatccgaa tacccaggtt ggcgcgctta tcagccagca gcactgggaa
     5701 aaagtctccg gctatatccg tctcggcatt gaagaaggcg ccaccctgct ggcgggcggc
     5761 ccggataaac cgtctgacct gcctgcacac ctgaaaggcg gcaacttcct gcgcccaacg
     5821 gtgctggcag acgttgataa ccgtatgcga gtcgcccaag aagagatttt cgggccggtc
     5881 gcctgcctgc tgccgtttaa agacgaagct gaaggcttac gcctggcaaa cgacgtggag
     5941 tacggcctcg cgtcgtacat ctggacacag gatgtcagca aagtgttacg cctggcgcgt
     6001 ggcattgaag ctggcatggt gttcgtcaac acccagaacg tgcgtgacct gcgccagcca
     6061 tttggcggcg taaaagcctc cggcaccggg cgtgaaggcg gtgagtacag cttcgaagtg
     6121 ttcgcggaaa tgaagaacgt ctgcatttcc atgggcgacc atccaattcc gaaatgggga
     6181 gtctgatatg ggtaagttag cgttagccgc caagatcacg cacgtaccgt cgatgtatct
     6241 ctctgaactg ccagggaaaa accacggttg ccgccagggc gcgatcgacg ggcataaaga
     6301 gatcagcaag cgttgccggg aaatgggcgt cgataccatt atcgttttcg atacccactg
     6361 gctggtcaac agtgcttatc acatcaactg tgcagaccat tttgaaggcg tctacaccag
     6421 caacgagctg ccgcatttta tccgcgatat gacctacaac tacgagggca acccggagtt
     6481 ggggcagctt attgccgatg aagccttaaa gctcggcgtg cgggcaaaag cgcacaacat
     6541 tcccagcctg aaactggaat acggcacgct ggtgccgatg cgctacatga atgaagacaa
     6601 gcacttcaaa gtggtctcca tttcggcttt ctgcacggtt cacgattttg ccgacagccg
     6661 caagctgggc gaagcgattc tgaaagcgat cgaacagtac gacggcaccg tggcggtcct
     6721 tgccagcggt tcgttatcgc accgctttat tgacgatcag cgtgcagaag aagggatgaa
     6781 cagctacacc cgcgagttcg accgccagat ggacgagcgc gtggtgaagt tgtggcgcga
     6841 aggccagttc aaagagttct gcaatatgct gccggagtac gccgactact gctacggcga
     6901 aggcaatatg cacgacacgg tgatgctgct ggggatgctc ggctgggata aatacgacgg
     6961 caaggtggag tttatcaccg agctattccc aagctctggc accggtcagg ttaacgctgt
     7021 tttcccgctt cccgcgtaag gacgttttat gccgcacttt atcgttgaat gcagtgacaa
     7081 catccgcgaa gaagccgacc tgccggggtt gttcgccaaa gtgaatccga cgctggcagc
     7141 cacgggtatt tttccgctgg cgggtattcg cagccgcgtg cattgggtcg atacctggca
     7201 gatggccgac gggcagcgtg attatgcctt cgtgcatatg acgttgaaaa tcggcgcagg
     7261 tcgcagcctg gaaagccgtc agcaggcggg tgaaatgctg tttgaactga ttaaaacgca
     7321 cttcgccgcc ctgatggaga gccgcctgct ggcgttgtcg tttgagattg aagagctgca
     7381 tccgacgctg aattttaaac aaaacaacgt gcacgcattg tttaagtagc gcgcagattg
     7441 cccggtggcg ctgcgcttac cgggcctaca aaaccccaaa ccgtacaccg taggccggat
     7501 aaggcgcagc cgcatccggc aatgccacag gatatcgcta tgttcgataa acacacccac
     7561 accctgatcg cccagcgtct ggatcaggca gaaaaacagc gcgaacagat ccgcgcgatc
     7621 tcgctggatt acccagagat caccatcgaa gacgcttacg cggtgcagcg cgaatgggtt
     7681 cgcctgaaaa tcgccgaagg ccgtacgctg aaaggccaca aaatcggtct gacctcaaaa
     7741 gcaatgcagg ccagctcgca gatcagcgaa ccagattacg gtgccctgct ggatgacatg
     7801 ttcttccacg atggcagcga tatcccgacc gatcgcttta tcgtgccgcg cattgaagtg
     7861 gagctggctt tcgtgctggc aaaaccgctg cgtgggccaa actgcacgct gttcgacgtc
     7921 tacaacgcca cggactacgt gatcccggcg ctggagctga tcgacgctcg ctgccacaac
     7981 atcgatccgg aaacccagcg cccgcgcaaa gtgttcgaca ccatttctga taacgccgcc
     8041 aacgccgggg tgatcctcgg tggtcgtccg atcaaaccag acgaactgga tctgcgttgg
     8101 atctccgccc tgatgtatcg caatggcgtg attgaagaaa ccggcgtcgc cgctggcgtg
     8161 ctgaatcatc cggcaaacgg cgtggcctgg ctggcgaaca aactcgcacc gtatgacgta
     8221 caactggaag ccgggcaaat cattctcggt ggttcgttca cccgcccggt tccggcgcgt
     8281 aagggcgaca ccttccacgt cgattacggc aacatgggtt ccattagctg ccgctttgtt
     8341 taaggagaga acgatggaaa acagctttaa agcggcgctg aaagcaggcc gtccgcagat
     8401 cggattatgg ctggggctga gtagcagcta cagcgcagag ttactggccg gagcaggatt
     8461 cgactggttg ttgatcgacg gtgagcacgc gccgaataac gtgcaaaccg tgctcaccca
     8521 gctacaggcg attgcgccct accccagcca gccggtagta cgtccgtcgt ggaacgatcc
     8581 ggtgcaaatc aaacaactgc tggacgtcgg cacacaaacc ttgctggtgc cgatggtaca
     8641 aaacgctgac gaagcccgtg aagcggtacg cgccacccgt tatccccccg ccggtattcg
     8701 cggtgtgggc agtgcgctgg cccgcgcctc gcgctggaat cgtattcctg attacctgca
     8761 aaaagccaac gatcaaatgt gcgtgctggt gcagatcgaa acgcgtgagg caatgaagaa
     8821 cttaccgcag attctggacg tggaaggcgt cgacggcgtg tttatcggcc cggcggatct
     8881 gagcgccgat atgggttatg ccggtaatcc gcagcacccg gaagtacagg ccgccattga
     8941 gcaggcgatc gtgcagatcc gtgaatcggg caaagcgccg gggatcctga tcgccaatga
     9001 gcaactggca aaacgctatc tggaactggg cgcgctattt gtcgccgtcg gcgttgacac
     9061 caccctgctc gcccgcgccg ctgaagcgct ggcagcacgg tttggtgcgc aggccaccgc
     9121 cgtgaagccc ggcgtgtatt aatgccggat gcgctgcact tatccggcct acactcgcac
     9181 caaaccgtag gccggataag gcgtttacgc cgcatccggc aaaaagccgt accaaatcgc
     9241 ggaccggata aggcgttacg ccgcatccgc cataaacctt gtcgtaccct acaaaaatcc
     9301 cattagagga agaaaaatga gcgacacctc acctgccata ccggagagta tcgatccggc
     9361 gaatcagcat aaagcgctga ctgccggaca acaggcggtt attaagaagc tatttcgccg
     9421 cctgatcgtc tttctgttcg tgctgtttat cttctcgttc cttgatcgca tcaacatcgg
     9481 ctttgccgga ctcacgatgg gacgcgacct cggtctgagc gccaccatgt ttggcctcgc
     9541 taccaccctg ttctatgccg cttatgtcat cttcggcatt cccagcaaca ttatgctgag
     9601 tattgtcggt gcgcggcgct ggatcgccac catcatggtg ctctggggca tcgcctctac
     9661 tgccaccatg tttgccactg gccccaccag cttgtacgta ctgcgtatac tggttggcat
     9721 taccgaagcc ggctttctgc ctggtattct gctgtattta accttctggt ttccggccta
     9781 tttccgcgcc cgtgccaacg ccttgtttat ggtagcaatg ccggtaacga cagcgttggg
     9841 ctcgatcgtt tccggctaca ttttgtcgct ggatggcgtc atggcattaa aaggctggca
     9901 gtggctgttt ttgctggaag gcttcccgtc ggtattactc ggcgtcatgg tgtggttctg
     9961 gcttgatgac tcaccggaca aagctaagtg gctgacgaaa gaagacaaaa aatgcctgca
    10021 agagatgatg gataacgatc gtctgacgct ggttcagcca gagggagcca tcagccacca
    10081 cgccatgcaa caacgcagta tgtggcggga gatcttcact ccggtggtga tgatgtatac
    10141 cctggcgtat ttctgcctga ccaacacact tagtgcgatc agcatctgga caccgcagat
    10201 cctgcaaagt tttaatcagg gcagcagtaa tatcaccatc ggcctgctgg ccgccgtacc
    10261 gcagatttgt accattctcg ggatggtcta ctggagccgt cactcagatc gccgccagga
    10321 acgaaggcat cacaccgccc ttccttattt gttcgctgcc gcaggttggt tactggcttc
    10381 ggcaactgat cacaacatga tccagatgct ggggatcatt atggcttcga ccggatcatt
    10441 cagcgcaatg gcgattttct ggacaacacc ggatcagtcc atcagcctgc gggcacgagc
    10501 gatcggtatt gcggtgatca acgccactgg caacattggt tcagcgttaa gtccgtttat
    10561 gatcggctgg ttgaaagatt tgaccggcag ctttaacagt ggattgtggt ttgttgccgc
    10621 gctgctggtg attggtgcgg ggattatctg ggcgattcca atgcagtcct cccgtccgcg
    10681 agcgaccccg taaggaacga cgatgtgtga ccgtcagatt gccaatattg atatcagcaa
    10741 agagtacgat gaaagcctgg gcacggacga tgtgcattat cagtcgttcg cccgcatggc
    10801 ggcatttttt ggccgccata tgctgccaca tcgccacgaa cagtactttc agatgcattt
    10861 cctcaatagc ggacagattg agctacagct tgacgatcat cgctactcgg tggaagcgcc
    10921 cctgtttgtc ctgacgccgc cgtcagtacc tcatgcgttt attacggagt ctgacgccga
    10981 cggtcatgtg ttgacggtac gggaagatct gatctggccc ctgctggaag ttctttatcc
    11041 gggcactcgg gaaaccttcg gcctgccggg ggcctgtcac tggcagataa acccgacgaa
    11101 ctggcggcgc tggaacacta ttggcaactg atagagcggg aatcggtgga acaactgcct
    11161 ggacgggaac acaccctgac gttactggca caggcagtgt tcaccctact gctgcgtaac
    11221 gcaaaactcg acgaccatgc cgccagcggg atgcgcggag aattaaaact gttccagcgt
    11281 tttaatatgc tgattgaaag ccattttcat cagcactgga cagtaccgga ttacgctaac
    11341 gaactgcata tcaccgaatc acgactcacg gacatctgcc gtcgctttgc caaccgtccg
    11401 ccaaaacggt tgattttcga caggcagctg cgagaagcca agcggctgct gctgttttct
    11461 gataacgccg tgaacaatat tgcctggcaa ctcggtttta aggatccggc ttattttgcg
    11521 cgctttttta atcgcttagt cggttgctcg cccagtgctt atcgcgccaa aaaagtacct
    11581 gtgacgtgaa aatcccttag ccttaacggg aaaccaggca ccacctgcta ttcccctttc
    11641 tccgcctgga gcaaggggat ttagtaacgt caaaaatcgc taaaagcgac ctcgatcaca
    11701 aaaccgtacc cgacctgaaa agtaccagcc attgtcccaa aagtctcttc ccgtaatcgc
    11761 ccctgcaagc tttaattgaa taacaaaaac aacacacaaa attaacaatc gtattccgat
    11821 taatactgta gaggtcgaca tgaaaccaga agatttccgc gccagtaccc aacgtccgtt
    11881 caccggggaa gagtatctga aaagcctgca ggatggtcgc gagatctata tctatggcga
    11941 gcgagtgaaa gacgtcacta ctcatccggc atttcgtaat gcggcagcgt ctgttgccca
    12001 actgtacgac gcgctacaca aaccggagat gcaggactct ctgtgctgga acaccgacac
    12061 cggcagcggc ggctataccc ataaattctt ccgcgtggcg aaaagtgcca acgacctgcg
    12121 ccagcaacgc gatgccatcg ctgagtggtc acgcctgagc tatggctgga tgggccgtac
    12181 cccagactac aaagctgctt tcggttgcgc actgggcgcg aatccgggct tttacggtca
    12241 gttcgagcag aacgcccgta actggtacac ccgtattcag gaaactggcc tctactttaa
    12301 ccacgcgatt gttaacccac cgatcgatcg tcatttgccg accgataaag taaaagacgt
    12361 ttacatcaag ctggaaaaag agactgacgc cgggattatc gtcagcggtg cgaaagtggt
    12421 tgccaccaac tcggcgctga ctcactacaa catgattggc ttcggctcgg cacaagtgat
    12481 gggcgaaaac ccggacttcg cactgatgtt cgttgcgcca atggatgccg atggcgtaaa
    12541 attaatctct cgcgcctctt atgagatggt cgcgggtgct accggctcgc catacgacta
    12601 cccgctctcc agccgcttcg atgagaacga tgcgattctg gtgatggata acgtgctgat
    12661 cccatgggaa aacgtactga tctaccgcga tttcgatcgc tgccgtcgct ggacaatgga
    12721 aggcggtttt gcccgtatgt atccgctgca agcctgtgtg cgcctggcag tgaaattaga
    12781 cttcattaca gcactgctga aaaaatcact cgaatgtacc ggcaccctgg agttccgtgg
    12841 tgtacaggcc gatctcggtg aagtggtggc gtggcgcaac accttctggg cattgagtga
    12901 ctcgatgtgt tctgaagcga cgccgtgggt caacggggct tatttaccgg atcatgccgc
    12961 actgcaaacc tatcgcgtac tggcaccaat ggcctacgcg aagatcaaaa acattatcga
    13021 acgcaacgtt accagtggcc tgatctatct cccttccagt gcccgtgacc tgaacaatcc
    13081 gcagatcgac cagtatctgg cgaagtatgt gcgcggttcg aatggtatgg atcacgtcca
    13141 gcacatcaag atcctcaaac tgatgtggga cgccattggc agcgagtttg gtggtcgcca
    13201 cgaactgtat gaaatcaact actccggtag ccaggatgag attcgcctgc agtgtctgcg
    13261 ccaggcacaa agctccggca atatggacaa gatgatggcg atggttgatc gctgcctgtc
    13321 ggaatacgac cagaacggct ggactgtgcc gcacttgcac aacaacgacg atatcaacat
    13381 gctggataag ctgctgaaat aacgcagcag gaggttaaga tgcaattaga tgaacagcgc
    13441 ctgcgctttc gtgacgcgat ggccagcctg tcggcagcgg taaatattat caccaccgag
    13501 ggcgacgccg gacaatgcgg gattacggca acggccgtct gctcggtcac ggatacacca
    13561 ccatcgctga tggtgtgcat taacgccaac agtgcgatga acccggtttt tcagggcaac
    13621 ggtaagttgt gcgtcaacgt cctcaaccat gagcaggaac tgatggcacg ccacttcgcg
    13681 ggcatgacag gcatggcgat ggaagagcgt tttagcctct catgctggca aaaaggtccg
    13741 ctggcgcagc cggtgctgaa aggttcgctg gccagtcttg aaggtgagat ccgcgatgtg
    13801 caggcaattg gcacacatct ggtgtatctg gtggagatta aaaacatcat cctcagtgca
    13861 gaaggtcacg gacttatcta ctttaaacgc cgtttccatc cagtgatgct ggaaatggaa
    13921 gctgcgattt aagctccagc cccttcacgt attcgtcgaa ggggcatttt tattgtgatc
    13981 tcaatcacat atccacccga tgaataattc ttgcccaacc gctcaccgcc attcctgacc
    14041 gcttacgtcc ctaaaataac cactcagtta tttaccttac tttacgcgcg cgtaactctg
    14101 gcaacatcac tgcaggatag cggtcaattt acctcctcaa acgcaacgca aacctagaac
    14161 ggcttcggcc tatgattaac aaattactct atgaattttg gtttgcagga aggcagcaag
    14221 tgagtgaatc cccgggagct tacaacagta agtgacagag gtgaacgaac gcagctgccg
    14281 cacctgtaag ccaaaagacg acgagtaaaa tgccaggttt tactatggat acgaaaaaac
    14341 tattcaagca cataccctgg gtgattctcg gaatcatcgg tgcattctgc ctcgcggtag
    14401 ttgcattacg tcggggggag cacgtcagcg ccctgtggat cgtggtcgcc tctgtgtcgg
    14461 tgtatctggt ggcgtatcgc tactacagtc tgtacatcgc ccagaaggtg atgaaactcg
    14521 accccacgcg cgcgacgcct gcggttatta acaacgacgg tctgaactac gttccgacca
    14581 accgttacgt gttgtttggt caccacttcg ccgctatcgc cggtgccggt ccgctggttg
    14641 gtccggtgct cgccgcgcag atgggctacc tgcctggcac gctgtggctg ctggcggggg
    14701 tagtactggc cggtgcggtt caggacttta tggtgctgtt tatctcctct cgccgtaacg
    14761 gcgcatctct cggtgagatg atcaaagaag agatgggacc agtaccgggg actatcgcgc
    14821 tgtttggctg tttcttaatc atgatcatca tcctcgccgt tctggcgctg attgtggtta
    14881 aagccctggc cgaaagtccg tggggtgtct tcaccgtttg ctcaaccgta ccgattgcgc
    14941 tgtttatggg tatctacatg cgctttatcc gtccggggcg tgtaggtgaa gtttctgtta
    15001 ttggtatcgt gctgctggtt gcctctatct atttcggtgg cgtgattgct cacgatccgt
    15061 actggggtcc ggcactgacc tttaaagaca ccaccattac cttcgcgctg attggctatg
    15121 cgtttgtttc cgcactgctg ccggtgtggc tgatcctcgc accgcgcgac tatctggcaa
    15181 ccttcctgaa aatcggcgtt atcgtcggcc tggcgctggg tatcgtggta ctgaacccgg
    15241 aactgaaaat gcctgcaatg acccagtaca ttgacggtac tggcccgctg tggaaaggcg
    15301 ctctgttccc gttcctgttc atcaccatcg cctgtggtgc ggtatctggc ttccacgcgc
    15361 tgatctcttc cggtacgacg ccgaaattgc tggctaacga aaccgacgcg cgtttcattg
    15421 gctacggcgc aatgctgatg gaatccttcg tggcgattat ggcgctggtt gctgcgtcca
    15481 tcatcgaacc gggtctttac ttcgcgatga ataccccgcc tgctggcctt ggcatcacca
    15541 tgcctaacct gcatgaaatg ggtggcgaga acgcgccgat catcatggcg cagctgaaag
    15601 acgttaccgc acacgcggcg gcgaccgtca gctcctgggg cttcgtgatt tcgccagagc
    15661 agatcctgca aaccgcgaaa gacattggtg agccttctgt cctgaaccgt gcaggtggcg
    15721 cgccgacgct ggcggtaggt atcgctcacg tgttccacaa agtgctgccg atggctgaca
    15781 tgggcttctg gtatcacttc ggtattctgt tcgaagccct gttcatcctg accgcgctgg
    15841 atgcgggtac tcgttctggc cgctttatgc tgcaagacct gctgggtaac ttcatcccgt
    15901 tcctgaaaaa aaccgactct ctggttgccg gtatcatcgg tactgcgggc tgtgtgggtc
    15961 tgtggggcta cctgctgtat cagggcgtgg tcgatccgct gggcggcgtt aagagcctgt
    16021 ggccgctgtt cggtatttcc aaccagatgc tggcagccgt agcgctggta ctgggcaccg
    16081 ttgtgctgat taagatgaag cgcacccaat acatctgggt aactgttgtt ccggctgtat
    16141 ggctgcttat ctgcaccacc tgggcgctgg gtctgaaact gttcagcacc aacccgcaga
    16201 tggaaggctt cttctacatg gcaagccagt acaaagagaa gattgctaac ggtactgacc
    16261 tgacggcgca gcagattgcc aacatgaacc acatcgttgt gaacaactac accaacgcag
    16321 gcctgagtat tctgttcctg attgtggtgt acagcatcat cttctacggt ttcaaaacct
    16381 ggcttgcggt gcgtaacagc gacaaacgta ctgacaaaga aactccgtac gttccaatcc
    16441 cggaaggcgg cgtgaagatc tcttcgcacc actaaccggt tgccggatgc gctgcgcgta
    16501 tccggcctac aaagtgtcta tcgtaggccg gataaggcgt ttacgccgca tccggcaaat
    16561 ccctagcccg gaccttccgg gctttacgca tatctggaag gcaaaatgtt tggtaactta
    16621 ggacaggcaa aaaaatatct cggccaggcg gcgaagatgt tgattggcat tccagactac
    16681 gacaactacg tcgagcatat gaagaccaac catcccgaca agccgtacat gagctatgaa
    16741 gaattcttcc gcgaacgcca gaacgcgcgc tacggcggcg atggtaaagg cggtatgcgc
    16801 tgttgttaat ggagagagca tgaacccgat tgcagttacc ctactcaccg gttttttagg
    16861 cgcgggcaaa accaccctgc tgcgccatat tcttaacgaa caacacggct acaagattgc
    16921 cgtgattgaa aacgaattcg gcgaagtctc cgttgatgat caactgattg gcgaccgcgc
    16981 cacgcagatc aaaacgctga ccaacggctg catctgctgt tcgcgctcca acgagctgga
    17041 agacgcacta ctcgacctgc tggataatct cgacaagggc aatattcagt tcgaccgtct
    17101 ggtcattgaa tgcaccggca tggccgatcc cggcccgatt attcagacct tcttctccca
    17161 tgagatttta tgccagcgct acctgctgga cggggtgatt gcgctggtgg atgcggtaca
    17221 tgccgatgag cagatgaacc agttcaccat cgcccagtcc caggttggct acgccgaccg
    17281 tattctgctg acgaaaaccg acgtcgcagg cgaagcagag aaactgcgtg aacgcctggc
    17341 gcgcatcaac gcccgcgcac cggtctacac cgttacccac ggcgatatcg acctgggcct
    17401 tctgttcaac accaacggtt ttatgctgga agaaaacgtc gtcagcacca aaccgcgttt
    17461 ccactttatc gcggacaaac aaaacgatat ttcgtcgatt gtggtggaac tggattaccc
    17521 ggtagatatc agcgaagttt cccgggtgat ggaaaacctg ctgctggaat cggcggataa
    17581 actgctgcgt tacaaaggga tgctgtggat tgacggcgaa cctaaccgcc tgctgttcca
    17641 gggcgtccag cgcctctaca gcgccgactg ggaccgcccg tggggcgatg aaaaaccgca
    17701 tagcacgatg gtgtttatcg ggattcagtt gccggaggat gagattcggg cggcgtttgc
    17761 gggactcaaa aagtaaccag ccctccaaca tcacaatacc tgagacttta tcaccctcat
    17821 attcctccag aatatgaggg cattcgggct taattatctc tgaacgtgca acacttccta
    17881 cgactcctta tgcgcctcca catcctgctt cgttaaccag agcgacttac gaccaaaatt
    17941 ttcgtacatt ttcagctgat cttcatagtg ctgatcaact gttccatcgg gagcaataaa
    18001 cccactctga ccgggtgcga ccacatccca ggcaagcacc ggacgatcgc ttgtcgttgg
    18061 tgagaaaaca atcatatcgt tttctgttcc acggttttga tactccgcct gatgacgcgt
    18121 ttcttccgct gcggcctgcg gtacatcaaa gaaattattt gcccggaacg ttaaggccat
    18181 tgcaggtgtt ttccagttac tcacattatt gccatagcgt ttggaaagag tctcccaggt
    18241 atcttccagc gcagccaaca caacctcctg ctgtggtttc ccagcaaaca gatcaaccgc
    18301 ctgtgggatt ggtgatttgt ctccctgcac cgcctcatac aaaatttttg ctccaacact
    18361 tatattcagc gaaccagttg ggccgtcctg ggttgtttcg tagccactgg cgctgtacca
    18421 cttatcaaat ggcataggta cggcagccac tacggtacgc ttcaacatac tggtcagcca
    18481 aacgttcagg atggcagagc ctggctgctg ccaggtttta ccatcatcat taagcaaatt
    18541 gatgccatcc caacgtgtta atgtatccac caactgacgg cgcggatcgc tctgtgtcaa
    18601 accagatgtc gctgcttgca gagtaggtaa aaaaagcctc aggttaagat cctgacgact
    18661 ggtttggcga ataacatccc atgcctgatc agcagttaag cgtggctttt gctcaagcag
    18721 tcggtcgatc tccgtaacgc gatct
//
LOCUS       DAJTMC010000078        17937 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.078, whole genome shotgun sequence.
ACCESSION   DAJTMC010000078 DAJTMC010000000
VERSION     DAJTMC010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17937)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17937)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17937
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.078"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            679..1044
                     /locus_tag="OO130_002336"
     CDS             679..1044
                     /locus_tag="OO130_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCR5365723.1"
                     /translation="MEKCDFYHIIVLSLNFPGYLKMEYGSTKMEERLSRSPGGKLALW
                     AFYTWCGYFVWAMARYIWVMSRIPDAPVSGFESDLGSTAGKWLGALVGFLFMALVGAL
                     LGSIAWYTRPRPARSRRYE"
     gene            1084..1788
                     /locus_tag="OO130_002337"
     CDS             1084..1788
                     /locus_tag="OO130_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HCR5365724.1"
                     /translation="MDLQHWQAQFENWLKNHHQHQDAAHDVCHFRRVWATAQKLAADD
                     DVDMLVILTACYFHDIVSLAKNHPQRQRSSILAAEETRRLLREEFEQFPAEKIEAVCH
                     VIAAHSFSAQIAPLTTEAKIVQDADRLEALGAIGLARVFAVSGALGVALFDGEDPFAQ
                     HRPLDDKRYALDHFQTKLLKLPQTMQTMQTARGKQLAQHNAHFLVEFMAKLSAELAGE
                     NEGVDHKVIDAFSPAG"
     gene            1855..3273
                     /gene="dcm"
                     /locus_tag="OO130_002338"
     CDS             1855..3273
                     /gene="dcm"
                     /locus_tag="OO130_002338"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HCR5365725.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHKEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMAKGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            3254..3724
                     /gene="vsr"
                     /locus_tag="OO130_002339"
     CDS             3254..3724
                     /gene="vsr"
                     /locus_tag="OO130_002339"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HCR5365726.1"
                     /translation="MADVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGSPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGREKLTDEALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(3713..4633)
                     /gene="yedA"
                     /locus_tag="OO130_002340"
     CDS             complement(3713..4633)
                     /gene="yedA"
                     /locus_tag="OO130_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HCR5365727.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVESWPPLMMAGVRF
                     LAAGILLMAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     VATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            4806..5723
                     /locus_tag="OO130_002341"
     CDS             4806..5723
                     /locus_tag="OO130_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HCR5365728.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKIRGQAH"
     gene            5802..5984
                     /locus_tag="OO130_002342"
     CDS             5802..5984
                     /locus_tag="OO130_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HCR5365729.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRSAEEV"
     gene            6155..7849
                     /gene="yedQ"
                     /locus_tag="OO130_002343"
                     /pseudo
     CDS             6155..7849
                     /gene="yedQ"
                     /locus_tag="OO130_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310721.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
     gene            complement(7846..8661)
                     /locus_tag="OO130_002344"
                     /pseudo
     CDS             complement(7846..8661)
                     /locus_tag="OO130_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
     gene            complement(8959..9175)
                     /gene="yodD"
                     /locus_tag="OO130_002345"
                     /pseudo
     CDS             complement(8959..9175)
                     /gene="yodD"
                     /locus_tag="OO130_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
     gene            9338..9526
                     /gene="dsrB"
                     /locus_tag="OO130_002346"
     CDS             9338..9526
                     /gene="dsrB"
                     /locus_tag="OO130_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HCR5365730.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            complement(9570..10193)
                     /gene="rcsA"
                     /locus_tag="OO130_002347"
     CDS             complement(9570..10193)
                     /gene="rcsA"
                     /locus_tag="OO130_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HCR5365731.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQHIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(10478..11263)
                     /gene="fliR"
                     /locus_tag="OO130_002348"
     CDS             complement(10478..11263)
                     /gene="fliR"
                     /locus_tag="OO130_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliR"
                     /protein_id="HCR5365732.1"
                     /translation="MMQVTSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFVIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEMFNLLADIISELPLI"
     gene            complement(11272..11541)
                     /gene="fliQ"
                     /locus_tag="OO130_002349"
     CDS             complement(11272..11541)
                     /gene="fliQ"
                     /locus_tag="OO130_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HCR5365733.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            complement(11551..12287)
                     /gene="fliP"
                     /locus_tag="OO130_002350"
                     /pseudo
     CDS             complement(11551..12287)
                     /gene="fliP"
                     /locus_tag="OO130_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly.; frameshifted; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
     gene            complement(12287..12652)
                     /gene="fliO"
                     /locus_tag="OO130_002351"
     CDS             complement(12287..12652)
                     /gene="fliO"
                     /locus_tag="OO130_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliO"
                     /protein_id="HCR5365734.1"
                     /translation="MNNHATVQSSTPVSAAPLLQVSGALIAIIALILAAAWQVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRSGRS"
     gene            complement(12655..13068)
                     /gene="fliN"
                     /locus_tag="OO130_002352"
     CDS             complement(12655..13068)
                     /gene="fliN"
                     /locus_tag="OO130_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HCR5365735.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSSKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMHRLSR"
     gene            complement(13065..14069)
                     /gene="fliM"
                     /locus_tag="OO130_002353"
     CDS             complement(13065..14069)
                     /gene="fliM"
                     /locus_tag="OO130_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HCR5365736.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            complement(14074..14538)
                     /gene="fliL"
                     /locus_tag="OO130_002354"
     CDS             complement(14074..14538)
                     /gene="fliL"
                     /locus_tag="OO130_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HCR5365737.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALNTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            complement(14643..15770)
                     /gene="fliK"
                     /locus_tag="OO130_002355"
     CDS             complement(14643..15770)
                     /gene="fliK"
                     /locus_tag="OO130_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook length control protein FliK"
                     /protein_id="HCR5365738.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLALLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLISDILADAQQADLLIPVDETLPVINDEQSTSTPLTTAQ
                     TMTLAAVADQNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDVPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTAAASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTVNHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            complement(15767..16210)
                     /gene="fliJ"
                     /locus_tag="OO130_002356"
     CDS             complement(15767..16210)
                     /gene="fliJ"
                     /locus_tag="OO130_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FliJ"
                     /protein_id="HCR5365739.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERLSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            complement(16229..17597)
                     /gene="fliI"
                     /locus_tag="OO130_002357"
                     /pseudo
     CDS             complement(16229..17597)
                     /gene="fliI"
                     /locus_tag="OO130_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
     gene            complement(17597..>17937)
                     /gene="fliH"
                     /locus_tag="OO130_002358"
     CDS             complement(17597..>17937)
                     /gene="fliH"
                     /locus_tag="OO130_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HCR5365740.1"
                     /translation="ASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
BASE COUNT         4404 a         4490 c         4717 g         4326 t
ORIGIN      
        1 caaccatctt gccgtagaaa tcaactttat tgccatcttt attgtaaatt tcagccgcat
       61 ttgctgcgcc agcaactaat aacgccggga ccagcattgc cagaactttt cttttcatta
      121 tgtattccct tgtgattata atcttcatga atatatcaat aagtgccgtt atccaaaaaa
      181 aagcacattt ggatactatt ctatgaagtt catttcattt taagtattac atgcaacaaa
      241 tgtatttaaa agatttcaaa atgtttctaa tctatttaat ataattcaga gatataaatt
      301 ataaatattc atgaaaatta taaatgtata tcaatatgtt atgagttgag cggaaacata
      361 gggggtatca atgattgttg cgccaaacgg ctggtgtgat caggcgcaca ttaatgaagg
      421 cataaaaaaa agccggcatc atgccggccc aaaaaatata aacaagacga taaaaggcta
      481 ataacggaag catcatgaca cagaataata aaaaggtata attgtaatca gtgctaatat
      541 attgattaaa tgaattaata aagccgatat caaagataaa taaaatcgct caaaacgatt
      601 tttcctgtat tcactgccgt tgcgcaaaat ttatctattt gttcaaaaaa tgattgagtc
      661 ttgactggct catccaatgt ggaaaaatgt gacttttatc acataatagt gctaagtctg
      721 aattttccgg gttatctcaa aatggaatac ggttcgacaa agatggaaga gagactctct
      781 cgcagccctg gcggcaaact ggctttgtgg gctttctata catggtgtgg ctatttcgtc
      841 tgggcaatgg cgcgttacat ctgggtgatg agccggatcc ccgatgcccc cgtgtcggga
      901 tttgaaagcg atttaggctc taccgcggga aagtggttag gggcactggt cggattttta
      961 tttatggctc tggtcggggc attgttaggc agcattgcct ggtacacccg acctcgtccc
     1021 gcacgtagca gacgctatga ataaaaactg ctaaggtggc gactctttgg caacggagcg
     1081 cgtatggact tacaacactg gcaggcacag ttcgaaaact ggttgaaaaa ccatcatcag
     1141 caccaggacg cggcccatga cgtgtgtcat tttcgccgcg tctgggctac ggcacagaag
     1201 ctggcggcag atgacgatgt cgatatgctg gtgattttaa ccgcctgtta ttttcacgat
     1261 attgtgagcc tggcgaaaaa tcatccgcaa aggcagcgtt cttcaatcct ggcggcagaa
     1321 gagacgcgtc gcctgctccg tgaagagttc gagcaatttc cggcggagaa aatcgaggcc
     1381 gtttgtcatg tcattgccgc tcacagtttc agcgcgcaaa tcgccccctt aacaacggaa
     1441 gctaaaatcg tccaggatgc ggatcggctg gaagccctgg gggcgattgg cctggcgcgc
     1501 gtgtttgccg tttcaggggc attgggcgtg gcgctgtttg atggtgaaga tccgttcgca
     1561 cagcatcgcc cgcttgatga taaacgctac gcgctggatc atttccagac taagttgctg
     1621 aagctaccgc aaaccatgca aaccatgcaa accgcaagag gcaagcagct ggcgcagcac
     1681 aacgcgcatt ttttagtcga gtttatggcg aagctcagtg ccgaactggc gggggagaat
     1741 gaaggtgtcg atcacaaggt gatagatgcg ttttcacccg cgggataagc gcgtggccct
     1801 aaatggctgt aattatgtta acctgtcggc catctcagat ggccggttaa atctatgcag
     1861 gaaaatatat cagtaaccga ttcatacagc accgggaatg ccgcacaggc aatgctggag
     1921 aaactgctgc aaatttatga tgttaaaacg ctggtggcgc agcttaatgg tgtgggtgag
     1981 aatcactgga gcgcggcaat tttaaaacgt gcgctggcga atgactcggc atggcaccgt
     2041 ttaagtgaga aagagttcgc ccatctgcaa acgttgttac ccaaaccacc ggcacatcat
     2101 ccgcattatg cgtttcgctt tatcgatcta tttgccggaa ttggcggcat ccgtcgcggt
     2161 tttgaatcga ttggcggaca atgcgtgttt accagcgaat ggaacaaaca tgcggtacgc
     2221 acttataaag ccaaccatta ttgcgatccg gcgacgcatc attttaatga agatatccgc
     2281 gatatcaccc tcagccataa agaaggcgtg agtgatgagg cggcggcgga acatattcgt
     2341 caacacattc ctgaacacga tgttttactg gccggtttcc cttgtcagcc attttcgctg
     2401 gctggcgtat cgaaaaagaa ctcgctcggg cgggcgcacg gttttgcctg cgatacccag
     2461 ggcacgctgt tttttgatgt ggtacgcatt atcgacgcgc gtcgtccggc gatgtttgtg
     2521 ctcgaaaacg tcaaaaacct gaaaagtcac gaccagggta aaacgttccg catcatcatg
     2581 cagacgctgg acgaactggg ctatgacgtg gctgatgcag aagataacgg gccggacgat
     2641 ccgaaaatca tcgatggtaa acattttctg ccgcagcacc gtgaacgcat cgtgctggtg
     2701 ggttttcgtc gcgatcttaa tctgaaagcc gattttactc tgcgtgatat cagcgaatgt
     2761 ttccctgcac agcgagtgac gctggcgcag ctgttggacc cgatggtcga ggcgaaatat
     2821 atcctgacgc cggtgctgtg gaagtacctc tatcgatatg cgaaaaaaca tcaggcgcgc
     2881 ggtaacggct tcggttatgg aatggtttat ccgaacaatc cgcaaagcgt cacgcgtacg
     2941 ctgtctgcgc gttattacaa agatggcgcg gaaattttaa tcgatcgcgg ctgggatatg
     3001 gccaaaggtg agaaagactt tgacgatccg ctgaatcagc aacatcgtcc acgtcggtta
     3061 acgcctcggg aatgcgcgcg cttaatgggt tttgaagcgc cgggagaagc gaaattccgc
     3121 attccggttt cggacactca ggcctatcgc cagttcggta actcggtggt cgtgccggtc
     3181 tttgccgcgg tggcaaaact gcttgagcca aaaatcaaac aggcggtggc gttgcgtcag
     3241 caagaggcac aacatggccg acgttcacga taaggccact cgcagcaaaa atatgcgcgc
     3301 gattgccacg cgtgatacgg cgattgagaa gcgcctcgcc agtctgttaa ccgggcaggg
     3361 cctggcgttt cgcgttcagg acgccagtct gcccggaagc ccggattttg tcgttgatga
     3421 atatcgctgc gtgatattta cccatggctg cttctggcat catcatcact gctatctgtt
     3481 taaagtgcct gcgactcgaa ccgagttctg gctggagaag ataggtaaaa atgttgaacg
     3541 cgatcgccgc gatatcagtc gtttgcagga actcggctgg cgcgtactga ttgtctggga
     3601 gtgtgcatta cgtgggcgcg agaagctgac ggatgaagcg cttaccgagc gtctggaaga
     3661 gtggatctgc ggcgaaggtg ccagcgcgca gatcgacacg caggggattc atttactcgc
     3721 ttgatgcgtc ctgaataact ggcgcaacta cgggttttgc cgggaagaga tattttccca
     3781 acgtgaccag taccaccgcg aagacaatta cgccgagcgc cagccattca atcttcgaca
     3841 gcgtctctcc gcccagtccc gtacccagca agaccgcgac caccgggtta acgtaggcgt
     3901 agctggtggc gagagccgga ctgacattac ggattaaata catataagcg ttgatggcga
     3961 taatcgaacc aaacagcgcc agatagccga ccgcaaggaa gcctgaaagg gaagggagcg
     4021 ccgtcagttt ttcacctgca atcatcgacg cgatcattaa caccacgcct gccgccagca
     4081 tctcaatcgc acccgccatc atccctacag gtaaggtaat gcgcgagcca taaaccgagc
     4141 caaacgccca gctaatcgag ccgattaaaa tcagaatcgc gccccacgga ttgccgctta
     4201 aatttccacc gctattgagc atgatgattc cggcaagccc aatggcgata cccacccatt
     4261 ccaatttgcg cgttttaatg ccaaacaggc ggctgaagca cagggtaaag aggggcacgg
     4321 ttgcaaccac aacggcggcg atgccggaag gaacattttg atgctcggca accgtcacca
     4381 tgccattacc gacagccagc aataacaggc caatcagcgc ggcattgagc agcggacgta
     4441 gcggggggag cttgtgtccg cgcagtagca aaaatgccat caataaaata ccggctgcca
     4501 ggaatcgaac gcctgccatc attaacggag gccagctttc cacgccaatc cgaatgacaa
     4561 aataggttga gccccaaatg atatacaacg caaacagcgc gccaaaaagc ggtaacaact
     4621 ggcggaaacg cataatccct cacggtggaa ataaaaaggt ggttcatagt aaacgtgaaa
     4681 atcattctgc tggcgagaga tataattgca cttgattgtt aaaaaaatgt tgacctgtga
     4741 agcaagtatc agagctgccg tttttgcttc atacttacac ccttcaacaa taaaaatgag
     4801 agggaatgct tttggccggg agtagtttac tgacgttgct cgatgatatc gccacactac
     4861 tggacgatat ctccgtgatg ggcaaactgg cggcgaagaa aaccgccggt gtattagggg
     4921 atgacttatc gctcaatgcg caacaagttt caggcgtgcg ggccaaccgg gaacttcccg
     4981 tggtctgggg cgtggcgaaa ggatcgctga ttaataaagt gattctggtg ccgctggcgc
     5041 tgatcatcag tgcgtttatc ccgtgggcga ttacgcctct gttgatgatt ggtggcgcgt
     5101 ttctctgctt tgaaggagta gagaaagtgc tgcatatgct ggaggcgcgt aaacataaag
     5161 aagatccggc gcagagccag cagcgtctgg agaagctggc ggcgcaggat ccgctgaagt
     5221 ttgaaaagga caaaataaaa ggggcgattc gtaccgattt tatattgtct gccgaaatcg
     5281 tcgccatcac gctggggatt gtggccgaag cgccgttgct taatcaggtg ctggtgcttt
     5341 caggcatcgc gctggtagtg accgtgggcg tctacggtct ggtaggggtt atcgttaaga
     5401 ttgatgacct ggggtattgg ctggcggaaa aatccagcgc gctgatgcag gcattaggta
     5461 aagggttatt gatcattgcg ccctggctga tgaaagcgtt atcgattgtc ggcacgctgg
     5521 cgatgttcct cgtcggcggc gggattgtgg tacatggtat tgcgccgctg catcacgcca
     5581 ttgaacattt cgccgggcag caaagtgcag tggtggcgat gatattaccg actgttttaa
     5641 atctgattct tggatttatc atcggcggca tcgtggtgct gggagtgaaa gccgtagcga
     5701 aaatacgcgg tcaggcacat taatactcgc acaggctatt atgcaaaatt cgccatcttc
     5761 aactaaggtg aacaagtttc actcgaaaaa aggaggcgag tatgagcttt atggttagtg
     5821 aggaagttac ggttaaagag ggcggcccgc ggatgattgt caccggatac tccagcggta
     5881 tggttgagtg tcgttggtat gacggttacg gggtcaagcg ggaagctttt catgaaaccg
     5941 agcttgttcc gggggagggg agtcgttctg cggaagaagt ttgaaagggt atcattaaca
     6001 ccaggccgga tcacttccgg cctggtgtgc caatgcctgc aatgaatgat agtcgcgtct
     6061 tatcatgcca ccattataaa attatcgctg ttttgcggta cgctatcgcc aggttatgcc
     6121 agaatcataa aaaagcaggt tgggagtcgt cagggtgcag cacgagacaa aaatggaaaa
     6181 ccagagctgg ttgaaaaaaa tcgcacgccg cctggggcct ggtcatgtcg ttaatctctg
     6241 ctttatcgtg gtattgcttt tttccacctt gctgacctgg cgtgaagtgg tagtgctgga
     6301 agatgcctat atctccagtc agcgtaatca tctggaaaac gtagccaacg cgctcgataa
     6361 gcatttgcag tataacgtcg acaaactgat ctttttgcgt aatggcatgc gcgaagctct
     6421 cgtagcgcca ctggatttca cttcactgcg taatgctgta accgagttcg aacagcatcg
     6481 cgacgagcac gcctggcaaa ttgaactcaa ccgacgacgc accctgtcag tcaatggcgt
     6541 atcggatgca ttagtcagcg aggggaatct cctgtctcgc gaaaatgaaa gcctcgacaa
     6601 tgaaattacc gctgcactgg aagttggtta cttgctgcga ctggcgcaca acacctcgtc
     6661 gatggttgaa caggcgatgt atgtctcgcg tgccggattt tacgtttcga cgcagccgac
     6721 cttgtttacg cgcaatgtac caacgcgtta ttacggctat gtcacccaac cctggtttat
     6781 cggccattcg caatgagaaa atcgtcaccg cgcggtacgc tggtttactt cgcaaccgga
     6841 acacgccagc aatactgaac cgcaggttac cgtcagtgtt ccggtagaca gtaataacta
     6901 ctggtatggc gtgctgggga tgagtattcc cgtgcgtacc atgcagcaat ttttaagaaa
     6961 cgccatcgat aaaaacctcg atggtgagta tcagctctat gacagtaagc tgagattttt
     7021 gacctcttcc aatcctgacc atccaacagg gaatattttt gatcctcgtg aactggcctt
     7081 gctggcgcag gcgatggaac atgacacgcg gggcggcatt cgtatggaca gtcgctatgt
     7141 tagctgggaa cgtctggacc atttcgacgg tgtgctggtg cgcgtccata cgctaagcga
     7201 aggcgtgcgc ggcgatttcg gcagtatcag cattgcatta accctgctgt gggcgctctt
     7261 taccaccatg ttactcatct cctggtatgt gattcgccgg atggttagca acatgtatgt
     7321 tctgcaaagc tcgttgcagt ggcaggcgtg gcacgacacc ttaacccgtt tatataatcg
     7381 tggcgcactg tttgaaaaag ctcgtccgct cgcgaaattg tgtcagacgc accaacatcc
     7441 tttttctgtc attcaggtcg accttgacca ttttaaagcg attaatgacc gctttagtca
     7501 tcaggcgggc gaccgtgttc tttctcatgc tgccggatta attagcagtt ccttgcgtgc
     7561 gcaggacgtt gccgggcggg tcggtggtga ggagttttgt gtgattctgc caggcgcgag
     7621 tctgacggag gctgcggaag tcgcagaacg tattcgcctg aagttaaatg aaaaagagat
     7681 gttgattgct aagagtacga cgatacgcat cagtgcctcg ttgggggtaa gtagcagcga
     7741 ggaagccggt gattatgatt ttgaacaact ccagtcactg gctgaccgtc ggctttatct
     7801 cgctaaacag gccgggcgta atcgggtatg cgcgagcgat aacgcttaac gagcggagaa
     7861 aaaatggtcc agcccttcac gccatccttc cggtccttca cgctgcgttc gccagacgcg
     7921 ggccggatcc tcatcatgca gatgcacccc ttcacggttt agccctttca caatcaccgc
     7981 gtaatccatt acctccagta agggcgcatc gtttggccca tcgcccaggc caagtgtggt
     8041 tgggcgtttg cctgataatt gttgataggt cgcgataatc cagttggcgg cctgatcttt
     8101 tccggcagag gcatccagga cgtgccagaa gcgcgcaccc tgcataaact gtaagcccag
     8161 ttcgttcaga cgagcggtaa attgtgccat acgctcgtca ctgtcgcgcc agattagcgt
     8221 taccgacgcc tcatgcagct gcgtcagcgc cgcctggcta cggcttaatc ccgtccattc
     8281 ggcgatggtt gcatcgtcga catcatcgaa agtcgtgaat ttaaaatgtt ctttctcgcg
     8341 tagcgtattt aaaacctggc tgatttcgcc atggctaata cctgagatga tgcgtggaaa
     8401 accgtctata tcctgccatt gttcagcaag ctggatcact gcgccatttt cggcaatcag
     8461 tggtacccct tgtaacccca acgttttttg caagtacagc atttccgctg aagttttact
     8521 gctacagaga atgacgggaa cattcgcttc gcgtaaacgg ctgagctagg gggcagccgg
     8581 ttgccagtca taactatgac tgtccagcag ggtgccatca agatcgctaa aaaccagtag
     8641 tggttgttga attgaaaaca tggtcgcgca gttctcccct taccaggatg attctcaaaa
     8701 cagcacaaat aatcaaagat gattttttcg ggagcgatcc cgttgacgcc agagccacca
     8761 aggggtgacg cacgtcacat ttctattcat aagtagcgtt aatcattcat atcgcgaata
     8821 ttttcttgtc agcgaaaaaa attgcggata aggtgatgaa cacatcagat ttcctggtgt
     8881 aacgaatttt ttaagtgctt cttgcttaag caagtttcat cccgaccccc tcagggtcgg
     8941 gattttttta ttgtgcattc aacgattcac ttcagagacg ctgaagtcat gaatatccag
     9001 ttcgaaggca tccgccaatt cacgccatgt atgatattca cgtccatcga cgctaacgtg
     9061 ttcagaatcg ttctggctgc gatgaactta tttcattgat cgccgccagc agggcatcga
     9121 catcgacgct gacctcacgt tttgcggtat cgctgtactc ttttgcggtt ttcatcatct
     9181 ttcctcgcaa ccgttactct gttgttgccg ccagtcgtta agtccgcctg gcatctctta
     9241 agtatagacc gtcgagaaaa tcagcactcg cgcgcgcctg gcgctgccag aaaaacgaaa
     9301 ttgttctaca ctggcacaaa gccacaggag gaaaacgatg aaggtgaatg atcgggtaac
     9361 agtcaaaacg gatggcggtc cgcgtcgtcc tggcgtggta ctagcagttg aggagtttag
     9421 tgaaggcaca atgtacctgg tttcgctgga agactacccg ctcggcatct ggttctttaa
     9481 tgaagcaggg catcaggacg gtatctttgt ggagaaagca gagtaatctt attcagcctg
     9541 actggtggga aaccaccagt cagaatgtgt tagcgcatgt tgacaaaaat accattagtc
     9601 acattatccg tcagtcggac gacatggtag ataacctgtt tattatgcgt tttgatctta
     9661 cgtttaatat tacctttatg cgatgaaacg gtcttggctt tgatattcat ttggtcagag
     9721 atttgaatgg ttccctgacc tgccatccac attcgcaaca tactcgattc ggttcggctc
     9781 aatgataacg tcggcatatt taaaaaagag gttatcgttg tctctttttt cagaatatcg
     9841 ccaaggatat cgtcgagaga ttccggttta atcgatttag aacttatcaa taaatttttt
     9901 ctgaccaata gatattcatc aaaatgaaca ttggcaattg ccataaaaac gataaataac
     9961 gtattgggat gttgattaat gatgtgcttg atgtgctgac tgttagaagc atcatggatg
    10021 aaacagtcct cattaataaa caccactgaa gggcgctgtg aatcacaagc tatggcaagg
    10081 tcatcaacgg tttcaatgtc gttgatttct ctttttttaa cccctctact caacagatac
    10141 ccggttaaac ctagtcgggt gtaactacat aaatccataa taatcgttga catggcatac
    10201 cctcactcaa tgcgtaacga taattcccct tacctgaata tttcatcatg actaaacgga
    10261 acaacatggg tcacctaatg cgccactctc gcgatttttc aggcggactt actatcccgt
    10321 aaagtgttgt ataatttgcc tggaattgtc ttaaagtaaa tgttgcgata tgtgagtgag
    10381 cttaaaacaa atatttcgcc gcaggagtat cctggaagat gttcgtaggt attatttttc
    10441 ggataatcct taggataaca tgataaacgt tacggaatta tattaatggc aattcactaa
    10501 taatatcagc cagcaaatta aacatctcac tgaataaatg ttcgcaaaaa ggtgcaatta
    10561 acggcattaa tgccgccatt aaagagatac cgacagtcag agttaatgga aatccaataa
    10621 caaaaatgga taattgcggg gccatacgat tcagtaaacc taatgccaga ttcagggtca
    10681 gcagcagagt aatgagcggt aacgccagca tcagcccgtt aaggaaaatc aaactccccg
    10741 ctttggtgag tgccagaaac gcattgctgt tcaacggttc gccaccaatt ggcagggtgt
    10801 gaaaggtatc gaccagcagt gaaatcaacc ataaatgacc gttaaatgtc aggaacagca
    10861 gtaacgccag catatccatg atacgcgcta aaacgggcat attgagatgg ctggccggat
    10921 cgacaaacgt cgcaaatgac agccccattt gcagaccgat aatttcgcca gcggttcgca
    10981 cagcggcaaa ggcaaattgc atggtaaaac caagcgcaat gccgatcagg atctgctgca
    11041 cggccagcca cagagcaaag aacgaaaaaa caggaacatc gttggcaggt aatgatgggg
    11101 caatgacgaa cgtgatcatc attgccagcc ccagttttac ccgtttcggc acgctgcgtt
    11161 cgctcagaat tggtgcggta gaaatgagcg ccagcacgcg cagtaacggc cagaagtaca
    11221 ggcttaacca ggaaagccat tgatcgcttg tcacctgcat cattgtacgg cctacccgat
    11281 gatatacggc aggttagtga acaaggtgcg gacgtaatcc agcaacagat tgagcatcca
    11341 cggtccggca ataataatgg caataaatac ggcgatgatt tttggaataa acgacagcgt
    11401 catttcgttg atctgcgtgg cggcctgcaa aatactgatg ataaggcccg tgaccagcgc
    11461 caccagcaac agcggggcag ccagtgccag cgcgactttc atcgcttcag tccccatcat
    11521 catgaccgat tcaggtgtca ttttgcctct ctagctgtaa aagctctgcg ccagcgaacc
    11581 gaccagcaat tgccagccat ccaccagtac aaacagcatc agtttaaagg gcagagcaat
    11641 ggtggcgggg ggaccatcat catccccaat gccatcaaca cgctggcaat caccaggtcg
    11701 ataatcaaaa aaggaatgaa aatcgtgaag cctatctgaa atgcggtttt caactcgctg
    11761 gtcacgtagg ccgggcgcaa aatgcgcatc ggcacggctt caggtccctg caacgggccg
    11821 gtattcgcta gtctggcaaa caaccctaaa tctgcctcac gggtctgacg cagcataaac
    11881 tcacgtagcg gctgcgcccc tttttccagc gcctcctgca ttgatatttt ctcttcgctg
    11941 aatggctggt acgcatcgac ataaattttg tcgatcaccg gtgacataat aaaaaaggtc
    12001 aaaaatagtg ccagccccag caatacctgg ttaggtggcg cggagggcgt tcccagcgcg
    12061 ttacgcaata aaccaaaaac aatgatgatg cgggtgaaac tggtcatcat cagtaaaatt
    12121 gccggaataa acgtcaacga ggtgatgaac accagcgtct gcaccgggag cgaccagctt
    12181 tgtccaccgc caggcagcgg ctggctggtg atacccggca gttgcgcgaa ggcgaggggc
    12241 gtaaccagcc agagaaggac aggtgcgaca gacaacaaac gacgcatcag gatctcccac
    12301 tacgcttaag caaatttttc atgaccgact gaaaatcggt ctgcggtatc tcttctgttg
    12361 gtgctgaagg gggaagttta tgcagcagat tgatttgccc tgcggtaacg ccgagcacca
    12421 gccgtgcatc ttccacatcg accaccacaa cccgttcacg cgcgcccagc gaagcactgg
    12481 cgctaatttt cagaccgtta acgccagtgc gtttcggggc aaatcccaac cgttttacct
    12541 gccaggcagc agcgaggatc agggcaataa tggcgatgag tgcgccgctg acctgcagca
    12601 gtggcgcagc agaaaccggc gtggaagatt gcacagtagc gtggttattc atcactaacg
    12661 gctcaggcga tgcattcgct cagacggagt aatgatatcg gtgatccgca cgccatattt
    12721 atcggcaacg accaccactt cgccctgggc gattaaataa ccgttgatca gaatatccag
    12781 tggttcgccc gccagaccgt ccagcgccac gacggaccct tgcgtcagac gcaacagctc
    12841 tttgatggtc atccgcgtac ggcccagctc gacggtcagc ttgaccggaa tatccattat
    12901 caggtcgata tcctgcaacg ttccgctgac atcaccaccg ccaaattgct ggaacaccgc
    12961 gtcggcagcg cttttgctgc tggttgattt ttgttcgctc aacgcttcag cccacagatc
    13021 gtccattgcg ccgttgttgt catcggccgg attattcatg tcactcattt gggctgttcc
    13081 tcgttcagag aatttaaaat cgggttaatc aaatgttcta tccgtaacgc atactgaccg
    13141 ttgagagtgc cgtactgact ggtcagcacc gggacgccgt caacatgggc gatgatgcga
    13201 tcaggttttt ctatcggcag gacgtcgccg ggtttcagtt ttaaaatctg cgacaggcgt
    13261 agcgagatat cggcaaagtt ggcgaccagc tccagctgtg aatgctgcac ctggcgcacc
    13321 aggttatcgc gccagttctg atcttcatta cgcgagtttt ccagcggcgg gttaaccaac
    13381 aattcccgta gcggctcgat catgctgaat ggcagacaga tattaaattc gccggtcagg
    13441 ttgccaatct ccacatggaa cggcgtgtta accacaatgt cgttcggcga ggtggtgata
    13501 ttggtaaatt tcacctgcat ttccgaacgc acgtactcaa cttccagcgg attaatcgcc
    13561 ttccaggcgt cgctatagcc ttcaagcgcc agctttaaca tgcggttgat gacgcgctgt
    13621 tcggtatggg taaactcgcg accttccact ttggtcggga agcgtccatc gccgccaaac
    13681 aggttatcca cggcgataaa caccagactc ggtgaaaaca ccaccagccc tgtgccgcgc
    13741 agcggtttca gatggataag gttcaggttg gtcggcaccg gcaggttgcg ggcaaattca
    13801 tggtacggct gaatgcggat ggccccgacg gttatatccg ggctacgacg cagcaggttg
    13861 aacagcccca tacgaaaatg gcgggcaaag cgctcattaa tgatttccag cgcctgcaaa
    13921 cgttcgcgca caacccgtcg ttgggtattc ggatcgtacg gacgaatgtc actttcgccg
    13981 ctaacactgg ctgtcggttc gtctttgact tcgctgtcac cattcaacag cgcatcaatt
    14041 tcagcttgag aaagaatact atcgcccatg tcgttatcgc agaataaaag cggtatacag
    14101 cacgtcggtg acatcctgtt tcggttgccc ggcaacaagc ggggtggaaa gcgtggtttt
    14161 aatctcggca atcaggtttt tcttgccttc ttctgtcgcc agtacggcag catcctgacg
    14221 cgaaaacagt aacagcaaac ggctacggac ttccggcaaa tactcactca gccgcgagcg
    14281 ggtagcttca tctttcaggc gcagggttat gccgatataa agcacgcgat ccgcatcgcc
    14341 caaattgacc gtgaaggtat tcagcgcgta gaagaccggg gagggtacga cgcgttgctg
    14401 cgctttgtcg tcagcggcaa cctgatgcga atgccagtag ctgtaacctg cgctggcaca
    14461 ggccgcgagg gtaatgaata ccagaatcgg gatccaaagc gatcgcttgc ttttcttgct
    14521 tatcgcgtaa tcagtcatgt gttgcggtct tcctgtgtcg ctactgctta tcggctaatt
    14581 atcctgcgtt ttgagcgcaa caaagcgggg aaaagacgcg gattacggtg ctacctctga
    14641 cgttaggcga aaatatcaac gccgctgttg cctgttacac gcccttgtaa agagacggga
    14701 accggaagcg tatcgtcgtc ttcccccgcc agaggttcat ggtttactgt gcgttggctt
    14761 tgctgttgtt gggaagcggc ctgctgctga ccactaaagc tttcgccact gatgttgctt
    14821 tgccctaact gaatgccact ttcggccagc tgcgtacgca gtaccggaag cgctgcttcc
    14881 agcgcggcgc gtacatgctg atgcggtgaa accatctgga tttgcgcctg gttatcatcc
    14941 actttgaggg agatttgcac ttcacctaaa tcctgcgggt gcagacgcaa ctctgcactt
    15001 tgttgcccct ggcgggtgaa cagcgaaata tgctggctta atgattgttg ccattcgtga
    15061 gaacccagcg gtgcactcaa aacaggcgcg gcgacggtgg gcagtggctg tgtctggtgt
    15121 ggagtaatta gcgggctggc ggcagcggtc accggtgacg gtgtgctgat gacttccgct
    15181 ttactctggg cttctgctac cagcggtgtt aatggctgag ctggcgtgcc gggggcatca
    15241 tcaggctgtg ctgttgtgag ttgcgcagaa gtcagttttg tgaagagcgt tggtttctct
    15301 gccggtaaca cggttgacgg cacatcagtc actttgggcg tattgtcaaa acccggcaac
    15361 atcgcaaaaa gggcgctcag gcttgcggtg acgtcttcat tcagatcatc cgctttttcg
    15421 tcttttgtcg tattttggtc agccaccgca gccaacgtca tcgtctgagc ggtggttaac
    15481 ggtgttgatg tggattgttc gtcgttgatg acaggcagtg tttcatccac ggggatcagt
    15541 aaatccgctt gttgcgcgtc ggcaagaata tcgctgatca gcggctcgcc ttttgtcgtg
    15601 ggcttatctg ttgccaccag caactggggg gccgctttgt cggtagttgt ctcgcctgct
    15661 aatgcttcgc tcaacaacgc gagaaaatct tgtgcagcat cgctggcttt gccgccaggc
    15721 aatgtggtgg tgtcaacgtc ggcggtaatt aagggcgcta agcgaatcat tcaggtttcc
    15781 tcatggcggc gcgctgggcg aactcatcca tctttttctg atcgaggcgg ttttctgcaa
    15841 gcagtgccgc cgtggatagc cgttcctgca gtgtctgcca ggcctgcaaa cgttgtttct
    15901 tttctcgcca gctgttcagg gcaatgtcaa ctttctgcgt ccactgatta agttgctggc
    15961 gatgctgagt aatggctttt tccagcgtct ggataaactg ctgatagttg atccagcggt
    16021 tgctggtcat cccggcactc atatcgctgt tgaggttatt gcgatattca ttctgataat
    16081 cgatcagcat tttgagctgt tcttccgcct gctgacaccc gcgacgcatt tcacccagca
    16141 ggcgcgcggc atcctctacc tctttttctg ccagatcttt cagggtcgcc agcgcgccat
    16201 gttctgccat ctgccgttat ctcctgggtt atgacactgt cgggaaaata cgctccagcc
    16261 cctggagaga cgcttcccag tccgcgcgtt caaaaatgcc ttgttgcaaa tagccctcca
    16321 gctgcggcca cagggcgatg gctttgtcga gcatcggatc gctgcctttg gcatacgcgc
    16381 cgacgctaac cagatcgcgg ttacgctgaa aactcgacaa cagctgtttg aaggtgcgca
    16441 ctcgcgcgta atgttgctca ctgatcaacg ccgtcattgc gcggctgatc gacgcttcaa
    16501 tatcgatagc cggatagtgc ccggcttccg ccagtcggcg agacagcaca atgtgaccgt
    16561 cgaggattgc ccgcgcagag tcggcaatgg ggtcctgctg gtcatcgcct tcagtgagca
    16621 cggtataaaa cgcggtaatc gatccgccgc cgctaatgcc atttccggca cgctcgacca
    16681 gtgccggtaa tttggcaaac accgacgggg gataaccttt agtggcgggt ggttcgccaa
    16741 tcgccagcgc aatttcacgc tgggccatcg cgtagcgggt gagggagtcc ataatcagca
    16801 acacatgctg accacgatcg cgaaaatctt cggcaatgcg cgtggcatag gcggcaccct
    16861 gcattcgcag gagaggagaa acatccgccg gagcggcaat caccacggag cgtgcacgcc
    16921 cttcggcacc gaggatattc tcaataaaat cttttacttc gcgtccgcgt tcaccaatca
    16981 aacccacgac aatgacatcg gcgcgggtgt aacgtgccat catccccagc agcacacttt
    17041 taccaacgcc ggacccggca aacagcccca tacgctgccc acgcccaacg gtaagcaggg
    17101 cattgattgg gcgcacgccg gtgtccagca catgttcaat cggtgtacgt tgcaacgggt
    17161 taaatggcgg ggtaatcagc gcaccggttt ccgtcgtatc gggggaggcc atcgagcggt
    17221 ttaccgctgc cgtccagaac acgacctaat aacgccggac cgagcggtaa ctgtttgccg
    17281 ctttgcagcc cttctgccga aatgtttttg gcataaacac gcgcgccggg caggacacct
    17341 tcgacttcct ccagcggcat taaaaacagc cgttgaccgt taaagccaac gacttcactt
    17401 tctacttcgt gcgtttcagt gccgttctgg cgctcaatca cacaggttgc gccgagcggc
    17461 aattgtaatc cggtggcttc cagcaccagc ccggtagcgc gggttaatcg cccgtagcga
    17521 cgtaccgcag gcaactgcgc cattttggct tcaaagttat ccagcgtggt tagccagcga
    17581 gtcaggcgcg tggtcattac accactcctg gtgctgccag acggcagagt tcttgccagc
    17641 gagtggcgac actggcgtcg agatcgcctt catcggcgga gactttacag ccgccaggat
    17701 ggagggtggg atcgccccgc aagcgccagc catgcaaact taaggtagcg ccgagcatat
    17761 catccacacg ttgcagatca tccgggtgca cgcgcagctg tggtttaccg ctgaataacg
    17821 gttcttgctg caacaactgt tggatctgtt tgatgagtgc cgagttatcc actgttggcg
    17881 tctgaccgat gacctgacgt gctgcctcca gcgccatctg catcaggcgc gaagcaa
//
LOCUS       DAJTMC010000079        17501 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.079, whole genome shotgun sequence.
ACCESSION   DAJTMC010000079 DAJTMC010000000
VERSION     DAJTMC010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17501
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.079"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            <1..744
                     /locus_tag="OO130_002359"
     CDS             <1..744
                     /locus_tag="OO130_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HCR5365741.1"
                     /translation="SSTDKPAKLKFHQYARATLLSNRGRDHNCDGRRTVEIHKLDLSD
                     WQAFNKLATKCNAYDGVTMNGDNSFGWNHEATLDNIHAQKYNKAYAGARLTAELKYLL
                     QDVESFEPNSKYTIHEVVLGPGYGTPDYTGQTIGYVVTLPAQMPNCWSSELPTIDLYI
                     DQLHTVTGVSNALGFIIAALLNAYSDLPHDLKIGLRSLSNSAAIYSGLGFERVPQDRD
                     IRSDRMHLTPANHPDLWTQENGEWIYLRN"
     gene            complement(815..1264)
                     /gene="rplI"
                     /locus_tag="OO130_002360"
     CDS             complement(815..1264)
                     /gene="rplI"
                     /locus_tag="OO130_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HCR5365742.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(1306..1533)
                     /gene="rpsR"
                     /locus_tag="OO130_002361"
     CDS             complement(1306..1533)
                     /gene="rpsR"
                     /locus_tag="OO130_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HCR5365743.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            complement(1538..1852)
                     /gene="priB"
                     /locus_tag="OO130_002362"
     CDS             complement(1538..1852)
                     /gene="priB"
                     /locus_tag="OO130_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HCR5365744.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            complement(1859..2254)
                     /gene="rpsF"
                     /locus_tag="OO130_002363"
     CDS             complement(1859..2254)
                     /gene="rpsF"
                     /locus_tag="OO130_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HCR5365745.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            2581..2856
                     /locus_tag="OO130_002364"
     CDS             2581..2856
                     /locus_tag="OO130_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR5365746.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            complement(2931..3482)
                     /locus_tag="OO130_002365"
     CDS             complement(2931..3482)
                     /locus_tag="OO130_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1440 domain-containing protein"
                     /protein_id="HCR5365747.1"
                     /translation="MAINGLFVRTDPQRRRYGVALLVGVLSGIISAFVKWGAEVPLPP
                     RTFSGGRDEFNPPYIFLRDYLGIDPTQTVYTFSEHIINPVMVTHIIFSLVFAIGYCIV
                     AEIFPKVKLWQGILAGLIVTVAVHGIFCPALNLTPPLTQLPFDEYASEILGHIFWFWI
                     IEIMRRDLRNRITHEPDPEVAIR"
     gene            complement(3579..4265)
                     /gene="ulaF"
                     /locus_tag="OO130_002366"
     CDS             complement(3579..4265)
                     /gene="ulaF"
                     /locus_tag="OO130_002366"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HCR5365748.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGKYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGDAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSFLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(4265..5119)
                     /gene="ulaE"
                     /locus_tag="OO130_002367"
     CDS             complement(4265..5119)
                     /gene="ulaE"
                     /locus_tag="OO130_002367"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HCR5365749.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETD
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVVKARDWVKARMA
                     KAGMVEAA"
     gene            complement(5129..5779)
                     /gene="ulaD"
                     /locus_tag="OO130_002368"
     CDS             complement(5129..5779)
                     /gene="ulaD"
                     /locus_tag="OO130_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HCR5365750.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYQRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            complement(5793..6257)
                     /gene="ulaC"
                     /locus_tag="OO130_002369"
     CDS             complement(5793..6257)
                     /gene="ulaC"
                     /locus_tag="OO130_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HCR5365751.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            complement(6267..6572)
                     /gene="ulaB"
                     /locus_tag="OO130_002370"
     CDS             complement(6267..6572)
                     /gene="ulaB"
                     /locus_tag="OO130_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HCR5365752.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            complement(6588..8036)
                     /gene="ulaA"
                     /locus_tag="OO130_002371"
     CDS             complement(6588..8036)
                     /gene="ulaA"
                     /locus_tag="OO130_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008786013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HCR5365753.1"
                     /translation="MSAQHSGCAEYPVTSSSGDRLYNIFTVFFNQVMTNAPLLLGIVT
                     CLGYILLRKSVSVIIKGTIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISD
                     TYASMMATIDRMGDAYSWVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGL
                     IAVTLFIFGYSMWTTIICTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASW
                     IAYKVAPFLGKKEESVEDLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAM
                     AGKVNWTVYILQTGFSFAVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAA
                     IYSFAPNAVVWGFMWGTIGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFG
                     GWRAALKICLVMGMIEIFGCVWAVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMA
                     VIIVIALAYMFFAGRALRAEEDAEKQLAEQSA"
     gene            8340..9404
                     /gene="ulaG"
                     /locus_tag="OO130_002372"
     CDS             8340..9404
                     /gene="ulaG"
                     /locus_tag="OO130_002372"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HCR5365754.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQAAPQEIRVQWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            9512..10267
                     /gene="ulaR"
                     /locus_tag="OO130_002373"
     CDS             9512..10267
                     /gene="ulaR"
                     /locus_tag="OO130_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HCR5365755.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERVGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(10264..11013)
                     /gene="yjfP"
                     /locus_tag="OO130_002374"
     CDS             complement(10264..11013)
                     /gene="yjfP"
                     /locus_tag="OO130_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR5365756.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSSDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNNIIAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            11195..11524
                     /gene="bsmA"
                     /locus_tag="OO130_002375"
     CDS             11195..11524
                     /gene="bsmA"
                     /locus_tag="OO130_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HCR5365757.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTNHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            11673..11948
                     /gene="yjfN"
                     /locus_tag="OO130_002376"
     CDS             11673..11948
                     /gene="yjfN"
                     /locus_tag="OO130_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HCR5365758.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(12065..13690)
                     /locus_tag="OO130_002377"
     CDS             complement(12065..13690)
                     /locus_tag="OO130_002377"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HCR5365759.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSLLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTTGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMCHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCV"
     gene            complement(13774..14832)
                     /locus_tag="OO130_002378"
     CDS             complement(13774..14832)
                     /locus_tag="OO130_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCR5365760.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNAIQERLISRFSELYSREPFYFCCCQDTDEDRSTVLYLQDCAQ
                     QAGQESRFIYIEDLGLGVGGVLTDLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQW
                     VEPLWKSILSNKGLMPLLWRFFPGHPNLLASWFEGEKPQIAAGESYVRKPIYSREGGN
                     VTIFDGQNNVVDHADGDYADEPMIYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIRED
                     NTLITKDTSRFVPHYIAG"
     gene            complement(14835..15473)
                     /locus_tag="OO130_002379"
     CDS             complement(14835..15473)
                     /locus_tag="OO130_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HCR5365761.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCGDSGDVDNDGDGTFYSTVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWLNTSGDYSWRSGSGKKESYSSGGFTTKKASTISRGGYGRSSSARGHWGG"
     gene            complement(15483..15881)
                     /locus_tag="OO130_002380"
     CDS             complement(15483..15881)
                     /locus_tag="OO130_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HCR5365762.1"
                     /translation="MHILDSLLAFSAYFFIGVAMVIIFLFIYSKITPHNEWQLIKNNN
                     TAVSLAFSGTLLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMPALSE
                     KIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            complement(15899..16558)
                     /locus_tag="OO130_002381"
     CDS             complement(15899..16558)
                     /locus_tag="OO130_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfK family protein"
                     /protein_id="HCR5365763.1"
                     /translation="MSGFFQRLFGKDNKPAIARGPLGLHLNSGFTLDTLAFRLLEDEL
                     LIALPGEEFTVAAVSRIDLGGGSQIFRYYTSGDEFLQINTTGGEDIDDIDDIKLFVYE
                     ESYGISKESHWREAINAKTMGAMTLNWQEKRWQRFFNSEEPGNIEPVYMLEKVENQNH
                     AKWEVHNFTMGYQRQVTEDTYEYLLLNGEESFNDLGEPEWLFSRALGVDIPLTSLHII
                     G"
     gene            complement(16609..17307)
                     /locus_tag="OO130_002382"
     CDS             complement(16609..17307)
                     /locus_tag="OO130_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HCR5365764.1"
                     /translation="MGILKSLFTLGKSFISQAEESIEETQGVRMLEQHIRDAKAELDK
                     AGKSRVDLLARVKLSHDKLKDLRERKASLEARALEALSKNVNPSLINEVAEEIARLEN
                     LITAEEQVLSNLEVSRDGVEKAVTATAQRIAQFEQQMEVVKATEAMQRAQQAVTTSTV
                     GASSSVSTAAESLKRLQTRQAERQARLDAAAQLEKVADGRDLDEKLAEAGIGGSNKSR
                     AQDVLARLQRQQGE"
     gene            complement(17380..>17501)
                     /locus_tag="OO130_002383"
     CDS             complement(17380..>17501)
                     /locus_tag="OO130_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2170 family protein"
                     /protein_id="HCR5365765.1"
                     /translation="KMMPLSSVGISSVQQEEYYIVFGALSLKSSLEDILLEIT"
BASE COUNT         4465 a         4677 c         4186 g         4173 t
ORIGIN      
        1 tcgtccaccg acaaaccagc aaaattaaaa tttcatcaat acgcacgcgc tacattactg
       61 tcaaatcgtg gaagagatca taattgcgat ggcagacgta ctgttgagat ccacaaacta
      121 gatctctctg actggcaagc attcaataag ttagcaacga agtgtaatgc ttacgatgga
      181 gtaacaatga atggtgataa ttcttttgga tggaaccatg aagctacatt agataatatt
      241 cacgcccaga aatacaataa agcttacgcg ggtgcgaggt taaccgcaga actgaaatat
      301 ttattgcaag atgtcgaatc cttcgaaccc aacagtaaat acacaataca cgaagtggtt
      361 ttgggacctg gttatggtac tccagactat accggccaaa cgataggata cgttgtaacc
      421 cttcctgccc aaatgcctaa ttgctggtca tctgaactac caacaatcga tctttatatc
      481 gaccaacttc acactgttac aggcgtttct aatgccctcg gattcatcat tgctgcattg
      541 ctaaacgcgt attctgattt accccatgat ttgaaaattg gtctgcgttc cttatcaaat
      601 tcagcagcaa tctactcggg tttaggtttc gagcgagtgc cgcaggaccg agacataagg
      661 agtgatcgta tgcatctaac gccagcaaac catcctgact tatggacaca agagaatggt
      721 gaatggatct acttgcgaaa ctaatacctg acgaaaaata gaaagcaaaa cgccgaccaa
      781 tggtcggcgt ttttacgtct cgttgaataa cgaattattc agctactacg tttacgatca
      841 ctttcgcgaa tacttcgctg tgaacctgga agctcacttc gtgttcgcca gtggtacgca
      901 gaacgccgtt cggcagacga acttcgctct tagccacttc aacgccagct gcagttacag
      961 cgtcagcgat gtcgcgagta ccgatggaac cgaacagttt accttcgtcg ccagctttag
     1021 acgcgatggt aacagtttcc agtgcattga ttttctcagc gcgagcatta gcagctgcca
     1081 gaacttcagc cagtttagct tccagttcag cgcgacgtgc ttcgaagaat tcaatgtttt
     1141 tcttggtagc tggaacagct ttaccctgcg gtaccaggaa gttacgagca tagcccgctt
     1201 taacgtttac ctgatcacct aggctaccca ggtttgctac tttatcaagc agaataactt
     1261 gcattacctt atcctctcaa agtcgtatta atggaccgtg accgattact gatggcgatc
     1321 agtgtacggc agcagggaca ggtagcgagc gcgtttgata gcgcgagcca gctgacgctg
     1381 gtattttgca cgggtaccgg tgatacggct tgggacaatc ttaccgcttt cggtgatgta
     1441 gtttttcagc gtagcgatat ctttatagtc gatctcttga acgccttccg cggtgaaacg
     1501 gcagaacttg cgacgacgga aataacgtgc catatggcta gtctccagaa tctatcaatt
     1561 caatctgctc ggcatgcaaa accattttgc tcagtccgtt ctttgccttg tggcatgaaa
     1621 tgaacccctg aacggttatg cgactgccga ccgttatact gtgagtaatg gcctggtttt
     1681 cgtgtccgct aacaataacg ggcatttggc accacgcctg ccggtgaaaa ccggcttcct
     1741 cctgcacaga acgatgctca agcacgaact ggcagtgagg aattcctgat ggactgacct
     1801 ttcgaagggg agtcctgcac acggtgccgg acaacaccag acggttggtc atcagaaatt
     1861 actcttcaga atccccagct tcagcatcat cagcggtttc gtttgcgaaa tcatcgcgac
     1921 gctcacggcg ctcgtctttc gctttaacca tcggagatgc ttcggtaaca gcgtgcttgg
     1981 tacgcataac catgctgcgg ataacggcat cgttgaagcg gaaggtagtt tccagctcat
     2041 cgatcacttc ctgcggagct tcaacgttca tcagaacgta gtgtgctttg tgcagtttgt
     2101 tgatcgggta agccagctga cggcggcccc agtcttccag acggtggatc ttgccttctg
     2161 caccagtgat ggcagcagtg tagcgctcga tcatgcccgg aacctgttcg ctctggtcag
     2221 gatggaccat aaaaacgatt tcgtaatgac gcatcgaatt gctccttacg gattattcag
     2281 cctcctgtct gggtcagccg aatcccgggg aggcaaggaa cgtgttaaag gtcggctgaa
     2341 aaatgacgcg ttatagtact tgtatgcccc actaaactca aggccagccg acaaataaat
     2401 ctcatctttc gctcaaaaga actaactcgc tgatttgaag ttcatcacat tccgtcacag
     2461 tgttttttcg aacaactgca tcagaaatgg tcaccatcaa cttctttatc attgattaaa
     2521 attaaatcag aagatcatca cagaggagag cacacaaagt cagccgtcga ggaggtatct
     2581 atgttcagtc gtgttttagc ccttctggct gtgcttttgc taagtgcaaa tacatgggca
     2641 gccattgaaa ttaataacca ccaggccaga aatatggacg acgtacagag cttaggcgtg
     2701 atttatatca atcataattt cgccactgaa agcgaagcac gtcaggcatt aaatgaagag
     2761 acagatgcgc agggcgcaac gtactaccac gtaattctga tgcgggaacc ggggagtaac
     2821 ggcaatatgc acgccagcgc ggatatttat cgctagcacc aggtataacc aacgaaacat
     2881 tgccatagtt tgctttgccc ccttcgcagg gggctttttt atacggttat tcaccggatt
     2941 gcgacttccg gatctggctc atgagtaatg cgattacgta agtcgcggcg cataatttct
     3001 attatccaga accagaaaat atgccccagg atttcagagg catattcgtc aaaaggcaat
     3061 tgagtgagcg gcggcgttaa attcagcgcc gggcaaaata taccgtggac tgccactgtc
     3121 acaataagcc ctgctaatat cccctgccat aatttcactt tcgggaaaat ttccgcgaca
     3181 atacaatacc ctatcgcaaa aaccagtgag aagatgatat gcgtcaccat caccgggttg
     3241 ataatatgtt ccgagaatgt atacacagtt tgggttgggt cgatacctaa ataatcccgt
     3301 agaaagatat acggcgggtt aaattcatcg cgaccaccgg aaaatgtgcg cggtggcaag
     3361 gggacttccg ctccccattt aacaaatgct gaaataattc ctgaaagtac acccacaagt
     3421 aacgcaacgc catagcggcg acgttgcgga tccgttctga caaacaatcc attgatagcc
     3481 ataaaaacac ctgttgttta tacgattttt taactgaagc ggccccggaa tcagaccaat
     3541 tacatcccgc aggaggtaat aattccgggg cgcactgctt acttctgccc gtaataggcg
     3601 ttaggaccgt gcttacgcat gaagtgttta ttcatgagga agctgtcgat gtgattcagt
     3661 tgtgggttaa tactgcgcgc aatccacgcc attttcgcca cttcttccat caccaccgcg
     3721 ttatgcaccg cgtcgtgggc atctttcccc caggcgaacg gcccgtgctg atacaccaca
     3781 attcccggcg tatgcagcgg ctcggcgtca cccagcgttt cgataatcac tttgccggtg
     3841 ttcagttcat actcgccctg cacctcttct tcgcttaatc cacgcgtaca cgggatgtcg
     3901 ccaaagaagt agtcggcgtg cgtggtgcct aacgccggga tcgccagccc cgcctgcgcc
     3961 catgcggtgg catgagtgga atgggtatgg acaatgccgc cgagcgacgg gtaacgacgg
     4021 tagagttcaa gatgcgtcgc ggtgtcagaa gacgggcgat acttcccttc taccaccttg
     4081 ccgctcatat cgaccaccac catatcgtct gctttcatgg tttcgtaggc aacgccgctg
     4141 ggcttgatca ccaccagccc gcgttcgcgg tcgatagcgc tgacgttgcc ccaggtaaag
     4201 gtcaccagcc catagcgcgg cagatccatg ttggcttcaa acacctgctg ttttagcttt
     4261 tgcattatgc cgcctccacc atgcctgctt tggccatgcg cgctttcacc caatcacgcg
     4321 ctttcaccac ttctgccgcc gggtcttccg ccgtttcgct ccacatctca atcaggtacg
     4381 gcccgcaata gccactctgt ttgagcgttt cgaagcaacg ttcgaaatcc accacacctt
     4441 cgccaaatgg cacgtttttg aagacgcccg gtttggtgtc tttcacatgc accgcgacga
     4501 tatgcccgat tccggcctgc aactccatct gcacatcgtt gtcccacgcc gacaggttgc
     4561 cgatatctgg gtagagctgg aaccacgggt tgttgagata gtgcgcgtaa cccagcgcct
     4621 tgctgatgga gttcatcaac ggataatcca tgatctccat cgccagcgtc acctgcgcgc
     4681 ggctcgccat ctcaacgctc tctttcaggc cgtcacggaa acgacgacgc gtttcgttat
     4741 tggcttcctg atagtaaacg tcatagcccg ccagctggat cacgcgaatg ccaacgtcct
     4801 gggcgaactg gatggcttta cgcataatct ccagcccctg cgcccgcacc gcgtcatctt
     4861 cgctgcccaa cgggaaacga cgatgggcag aaaggcacat ggacggcaca cgcacgccgg
     4921 tttcaacaat cgcattcacc agcgccagac gctgctcgcg gctccagtcg aggcgcgaca
     4981 ggcgctcgtc agtttcatct accgacatct cgacaaaatc gaagcccaac gtttttgcca
     5041 gtcgcaggcg ttccagccag cactccccgg cggggagcgc tttttcatag atgccaagcg
     5101 ggatttgttt ggacaacata tccgctcctt agccccacag ttcggcgatg gaacgtttga
     5161 actgacgcgc ggcttcaacc ggagaagcgg catcacggat gctgcgaccg gcgataaaca
     5221 catgaattgg aatgcctttg aacagcggca gatcttccag cgccaggcca ccggtgacgg
     5281 tgactttgaa gcccatatcg gaaagacgtt tgatcgcagt gatatcggct tcaccccacg
     5341 ccacgccagc ggcctgcgcg tcacggctgc gctgataaac cacctgctga atacctgcgt
     5401 cgcgccactg ttgcgcctgt tcccaggtcc agtaaccggt cagttcgatc tgcacgtcgc
     5461 cgttaaactc ttttgctacg tccagcgcac ctttggcggt gttgatatcc gcacaacaaa
     5521 tcacggtcac ccagtcagcg ttggcttcga agcacatacg agaaagaatt ttgcccgcat
     5581 cggcaatttt ggcgtctgcc agcacgattt tgtgcgggta gagcgctttc agatcgcgaa
     5641 ccgcacgcac gccttcgccc acgcacagaa tggtacccac ttcgataatg tcgacttctt
     5701 cggcaatcag gcgagtggtt tcgtaggcgc tatccatagt ctggttgtcc agcgcgactt
     5761 gcaacatcgg taatgacatc ttcaattcct tcttaagctg ccgcgttggt gcggtcaatg
     5821 agatccagta cttcctgctc ggtacggcag gcacgtaaac ggtcgaaatt ttcttcatct
     5881 tcaaacaggt tgacgatctg catgatgccc acttcctggt gcgtgttggc atcgaccgcc
     5941 gccatggtga tgaggatatc caccgggtcg ttatcttcgt ggttgaactc cagcggcttt
     6001 ttcagcgtca ccagcgcaaa accggttttc ttaacgccct cttccgggcg accgtgcggc
     6061 attgccaggc ctggtgcgag cacaaagtac ggaccgtgct gttcaaccgc atccagaatc
     6121 gcctggtagt aacgcggctc gacaacatcc gccgccacca gcaggtcaac gccgattttt
     6181 actgcgtcct gccatgtctc agcttcagcc tgcaggcgga tggatttatt ctccgccagc
     6241 gaatcacgta atttcatggc gcgtccttac ttcacatcct gcgggaaatg cgctttgatc
     6301 acttccagca gtttcgggcc aaagtcggca ggagagagca tgttgcgcac gccaaccacg
     6361 tatttgttgc cggtcacggt gatttcgccc gcaatgtgcg tagaagcgat gatgatatcc
     6421 gcgccgctca actcgctttt gtattcgcca accgcgcagc tatttaccgt gtggtcaatg
     6481 tttgattggg ttaaaaactg gtccactttc atcttcatga tcatggaact gccttgtccg
     6541 ttgccacaca cagccagaat acgtacggtc ataatcaaaa ctccttatta agcagactgt
     6601 tctgccagtt gtttttctgc atcttcttct gcgcgcagtg cgcggccagc gaagaacata
     6661 taagccagtg caattacaat gatgacggcc ataaaggcga taccgatgga gaagaagccc
     6721 tgcatcatcg gcggtgccag aatcgaccag tccgccatgc ccatccaggc gctcataccg
     6781 gtgagtttca ccgcccagac gcagccaaag atttcaatca tccccatcac cagacatatc
     6841 ttcagcgccg cacgccagcc gccgaagtgg ttagcgaaca cgccgatggt ggcgttggag
     6901 aagaacatcg ggataaagcc gggaataatc aggatcgagg aaccgcaggc gaccaggatg
     6961 ccaaccgcaa tcagctgacc gatggtgccc cacataaagc cccaaaccac ggcgttcggc
     7021 gcgaagctat agatagccgc acagtcaatc gccagaaccg caccagggat caggcgctgg
     7081 gagataccgt taaatgcttc agagagttcc gccacaaaca tgcgcacgcc ctgagtgatg
     7141 atgaagatcg ccaccgcgaa ggagaaacca gtttgcagga tgtacaccgt ccagttcact
     7201 ttgcctgcca tcgcctgcac ggtgtcgata ccgaaggaga gtagaatggc accaaagaag
     7261 atggtcatca caatcgccgt ggagacgatg ttgtcgtgga aaatattcag ccagcctggc
     7321 agtttgaggt cttcaacgct ttcctctttt ttgccgagga acggcgcaac tttataggca
     7381 atccatgatg caaactgttg ctggtgaccg atggagaaac cgcagccgtc cgtcacttcc
     7441 tgagtcggct tgtacatcat attggaggta atgccccagt agagcgaaac cagaatcgcc
     7501 gtgcagataa tggttgtcca catggagtag ccgaaaataa acaacgtaac ggcaatcagc
     7561 cccgcctgct ggaacatgat atgaccagtc agcatgatgg tacgaatacc ggtaatgcga
     7621 cgcagcagca cgtaacagat gttcagcgcc agcgctaaca acaccgcgta acctacccag
     7681 ctataggcat cgcccatgcg gtcgatggtt gccatcatcg acgcgtaagt atcggaaatt
     7741 gcgccgttaa tgccgtagac ttcggacatt ttcgccacca ccggtttgaa ggtgctggtg
     7801 aggatgccgg accctgcctg caacaacatg aaaccaatta tggttttaat cgtgccttta
     7861 ataataacgc tgacactttt gcgcagtagg atgtagccca gacaggtcac aatacccagc
     7921 aacaacgggg cattggtcat gacctggtta aaaaacacgg taaagatgtt gtagagacga
     7981 tctcctgaag aagaggtaac cgggtattcc gcacacccgg aatgttgtgc actcactcta
     8041 attttcaaaa gcaatcacaa caagattatt tatgattaaa tgtgacgcga cccgcaaata
     8101 attccatacg gatagagtgg gatccacaca acgaaacaat tatttattta aatttagtta
     8161 aattcattta gttaatttaa ctatacattc cattaaatct ttccacactc aaaatcaaca
     8221 acaacttccc tggcaaaaag tcaaaaaatg gtcattgcct tcctgaaaaa atagtgtcag
     8281 gattaatcaa aaccaatcac atattgattc gaattgaact atcatgaagg taaatggcga
     8341 tgagtaaagt gaaaagtatc acccgagaat cctggatcct gagcactttc ccggagtggg
     8401 gtagctggct gaatgaagaa attgaacaag agcaggtcgc ccctggcaca tttgcgatgt
     8461 ggtggctagg ctgcaccggg atctggttga aatcagaagg tggcaccaac gtttgcgttg
     8521 atttctggtg cggcactggc aaacaaagtc acggtaaccc gttaatgaaa cagggtcacc
     8581 agatgcagcg catggctggc gtgaaaaaac tgcagccaaa cctacgcacc accccgtttg
     8641 ttcttgatcc gtttgcgatt cgccagatcg acgcggtact ggcgactcac gatcacaacg
     8701 atcatatcga cgttaacgtc gctgctgccg tgatgcagaa ctgtgctgat gacgtaccat
     8761 tcattggacc gaaaacctgc gttgatttgt ggattggctg gggcgtaccg aaagagcgtt
     8821 gcatcgtggt caaaccgggc gatgtggtga aagtgaaaga cattgaaatt catgcgcttg
     8881 atgctttcga ccgtactgca ctgatcaccc tgcctgccga tcaaaaagcg gctggcgtac
     8941 tgccggacgg catggacgat cgcgcggtga actatctgtt caaaacgcct ggcggctccc
     9001 tgtatcacag cggcgactcc cactactcta actattatgc gaagcacggt aacgaacatc
     9061 agatcgacgt ggctttaggt tcatacggcg aaaacccgcg cggtatcacc gacaaaatga
     9121 ccagcgccga tatgctgcgt atgggtgaag ccctgaatgc gaaagtggtt atcccgttcc
     9181 accacgatat ctggtcaaac ttccaggccg ctccgcaaga gatccgcgtg cagtgggaga
     9241 tgaaaaaaga tcgtctgaag tatggcttca agccgtttat ctggcaggtt ggcggcaaat
     9301 ttacctggcc actggataaa gacaacttcg agtaccacta tccgcgcggt ttcgatgatt
     9361 gcttcactat tgaaccggat ctgccgttca aatcattcct gtaatctgtc gtttataccg
     9421 gatagcgtgg agaagttcgc gctatccggc gtagtatttc ctggctattt caaatatcat
     9481 ctaaaaaaat caaattttat cggaatagct catgactgaa gcacaaagac atcaaatcct
     9541 cctggaaatg ctcgcacaat tgggctttgt gaccgttgag aaagtcgttg agcgtctggg
     9601 aatttcgcct gccactgcgc gacgcgatat caataaactt gacgaaagcg gcaaactcaa
     9661 aaaggtgcgc aatggcgcag aagctattac ccaacagcgc ccgcgctgga cgccgatgaa
     9721 tctgcatcag gcgcagaatc acgatgaaaa agtacgtatt gctaaagcgg cctcgcagct
     9781 ggttaatccg ggcgaaagcg tagtcatcaa ctgcggctcc accgcgtttc tgcttgggcg
     9841 ggaaatgtgt ggcaagccag tgcaaatcat cactaattat ctaccgctgg caaattacct
     9901 gatcgatcaa gaacatgaca gcgtgatcat tatgggtgga cagtacaaca aaagtcagtc
     9961 catcacttta agcccgcagg gcagcgaaaa cagtctctat gccgggcact ggatgtttac
    10021 cagcggaaaa gggctgaccg cagaagggtt gtataaaacc gatatgctga cagcaatggc
    10081 agagcagaag atgctgagcg tggtagggaa actggtggta ctggttgata gcagtaagat
    10141 tggcgaacgc gtgggaatgc tttttagccg tgccgatcaa atcgatatgc ttatcaccgg
    10201 caaaaatgct aacccggaaa tcctgcaaca actggaagcg caaggggtca gcattctgcg
    10261 tgtttaaaga tgctggcgga aaaatgtcac ggcagcatct aacgcctcag gcgtaatgcg
    10321 gtgacgcacg cctggctgcc atgaacaggt tagcagttta tcccgtcccg tctcgcttaa
    10381 ggcctgctgc aaacgtagtg attcgtcggc aggcacaacg tcatcgtcga ggccatgcca
    10441 cagcagtaga ggtctgtccc caagttgttc caggtggttt gtcgcttccc actctgccag
    10501 cggcgcgata atgttattga attcattctg ctgtgctgcc gtttcaggta tcagcggtgg
    10561 aaacagtgaa cgggcgagcg atgtaaaata gcccgatccc atcatgctgg cggtacattt
    10621 caccgtgggg tggcgagcgg taattcccag tgccgtcatc gcgcccatcg acgcgccacc
    10681 gactgccaga cggtcatcaa gcagccagtt ttcttcggct attgccgcac gtaaagtagt
    10741 gaattcctgc atactttgta gcaagatttg ccagaattga tttaaccgcc gcgcttcgtc
    10801 gctactaaaa cggctaccgt gatcgggcgc atccggcatg atcacccgca aaccagcctg
    10861 cgccagcgca acggcaaaat agctatacac caggctggat gaagtaaagc cgtgataaaa
    10921 aattacgcac ggtaacgggg tatctttttg cccgacagga taagcatgaa gaacgggaat
    10981 atctgccagc tcgcgtgatt ctatttcaat catctgtctc tcctttttgt ctggtatgac
    11041 atgaagaaat ccttagcaca agtttctgct ggcggtatta gttcataatg ttgagatgtg
    11101 ggttacgctt tcgtcacgtt ttcattcgaa aatcgcgtac caggtaacaa ttcgggaaca
    11161 ttcccccaaa gttaaattaa taaggcacta cactatggtt agcaggaaac gtaatagcgt
    11221 tatttaccgg tttgccagtt tattattggt gttgatgtta agtgcctgta gcgcactgca
    11281 aggtacgcca cagccagcac cgccagtaac gaaccatccg caagagattc gccgcgacca
    11341 gacgcaaggg ttacagcgaa taggtagcgt aagcacgatg gttcggggtt ctccggatga
    11401 cgcattagca gaaatcagag caaaagctgt cgctgcaaaa gctgattatt acgttgtcgt
    11461 catggtggac gaaaccatcg tgacaggaca gtggtattca caagccattt tgtaccgtaa
    11521 ataaatcaga caacttacat agtcagcttt acattgcttt gcgtccctat gcgtttacct
    11581 gtgcacaata aattaccggc tggaagcctg ggcgtttatt accgcgatgg aatgccctgc
    11641 aacatgtggg gaaacgaaaa atggagctga cgatgaaaca attacttgct tcaccctcgc
    11701 tgcaattagt gacttatcct gcgagcgcca cggcacagtc tgcagaattc gccagtgctg
    11761 actgcgtgac gggcctgaat gaaataggtc agatctcagt tagcaatatt tcaggggatc
    11821 cgcaggacgt ggaacgtatc gtggcgttaa aggccgatga acaaggtgcg tcatggtacc
    11881 gcattattac catgtacgaa gaccagcagc ctgataactg gcgcgtacag gcgatactgt
    11941 atgcatgagt gtcatctaca gcaaatgaac aatgccggat tcggcgcgag cgccttgtcc
    12001 ggcctacaat tttcacatat ttcattgagt taatcgaaac cagcgtcgca tcagtcgtat
    12061 acgcttacac acacactccc cccgtcgccc gcagcaataa atcattctgg atctgctctg
    12121 acaaccgtac gccgccgcgc gtatctaaca tcacctgaca ccacgcctgc gccattggcg
    12181 gagaagcata tttcaacatt tgcgcaccgc agcccagcag gaatagctga tgagtaatct
    12241 ctcgccccag ttcttcagct ggcttacgca gctgctgctg taaacgacga accgcgcgat
    12301 caaaatagcg atcctgccct ttcacttcca caaatgcttc cgacaataag tcgtatacgc
    12361 ccgcttgctt attgagaacg cgcaacacat ccaggcacat aatattgccg gaaccttccc
    12421 aaatactgtt taccggcatc tcccggtaaa gccgcggcag ctcgctctcc tcgcaataac
    12481 caatgccgcc cagcacctcc atcgcttcgg ccacaaacgg catacctctt ttgcagatca
    12541 caaattttgc cgcaggcgta aataaacgcg cccacagggc ttctttggca tcggcacgcc
    12601 ggtcccacgc tcgcgcaaga cgaaacagca acgccgtttg cccttcaagc tgaagtgcca
    12661 tgcgacttaa gacatgacac ataaggggct gttggatcaa tggattacca aaaacatggc
    12721 gttgatgtgc atgataaatc gccagcgaaa atgcacggcg catcatggca tggctaccca
    12781 gggcgcaatc aaaacgcgtc atcccaccca ttttcaggat cagacgaatt ccttcccctt
    12841 cctgccccaa caaccagcca atggcatcct gaaactccac ttcgcaactg gcgttagagc
    12901 gattacccag cttatctttc agccgctcga ggcgaatcgc gttgcgttgc ccatcaggca
    12961 aaaagcgcgg cacaaaaaag caggacaaac cgcccgtagt ctgcgccagc accagatgcg
    13021 catcgctttg cggcaccgag aaaaaccatt tatgccccac cagccgataa gagccatctt
    13081 ccagacgctc tgcacgggtg gtgttgctca tgacatcgga accgccctgc ttttccgtca
    13141 ttcccatgcc aatcaacaaa ccgcgttttt gcccacctgg caataagaga gaatcgtagc
    13201 gatcgctcag cagcggcgtg gtccagtcct gaaacggcgt gggtaacatc tgtaacaaca
    13261 atggcgtggc ggcaaaggtc atcgttattg gacataacga cccagcctca acctgcgcat
    13321 gtaacataaa acgcgccgcg cgcgccacaa atgcgccgga gcgagcgtct tcttcccagg
    13381 caagattgtg cacccgattg gtacataacg cctgcatcag caggtgccag gcggggtgaa
    13441 aacgcacatc gtccagacgg cgtccttgcg catcgtagcg caataattca ggcggattca
    13501 cattcgccag ccgccccagt tcaagagatt cagccgttcc taactgctga ccaatactgg
    13561 ctagaaaatc gctatcccag ccagcacctt cacgcgttac cgcttcacag agcgcgccat
    13621 cagacaggta taagttgctg ttatttaatg gtataggttg attaaaaacg gtgtgagttt
    13681 gccagtgcac tgtgtctccc tccatcaatg gcagtcaccc ttagtatggt cactgccatg
    13741 gattcctgac agaaagagat tgctaaaaca ttcttatcca gcaatgtaat gcggaacgaa
    13801 acgtgaggtg tctttggtga tcagcgtgtt atcttcacgg atccccattc cgcacgcttc
    13861 atcatcgaca atccagctac cgatgagtgt gtagctatcg ccaaatcgcg gcagaggttg
    13921 aaacgcctgg tagatcatcg gttcatcggc gtaatcacca tcagcgtggt caacgacgtt
    13981 attctgaccg tcgaaaatgg tgacgtttcc gccttcgcgt gagtagatcg gtttacgcac
    14041 atagctttcg ccagcggcaa tctgcggttt ttcgccctca aaccaggatg caagaaggtt
    14101 aggatgacca ggaaagaagc gccaaagcag cggcattagc cctttattac tcaagatact
    14161 tttccataac ggctccaccc attgctcgcg acgcttgcgc agcagcggac cgttatcgtc
    14221 acgcatcatc cactccagcg gatacagctt aaatgcacgc tggatgacat tatcatcaag
    14281 atcggtcagt acgccgccga cgcccaaacc gagatcttca atgtagataa accgcgactc
    14341 ctgccctgcc tgctgggcgc agtcctgcaa gtacagcacg gtactcctgt cttcatcggt
    14401 gtcctgacag cagcaaaaat aaaacggttc ccgactgtaa agctcactaa agcgcgaaat
    14461 caggcgttcc tgaatggcgt tgtactccag cagcttcacc ggcgcattgc cacaccaggc
    14521 aaaatccatc cggccataca gcgaaggatc gcgtgcgcgc cagctttcgg cgatcacatc
    14581 ccagtacaac ggcgggatcg ccagttgcgt caggatctct tcgtctttaa ccgcgcgatc
    14641 caccacctca aggcacatct gatgcagttc cgcagtcggt ttttcgatct gctcttcaat
    14701 ctggcgcaga gtaaaacggt aagcccgact ctcgtcccaa tagatttcat tgtcgatgat
    14761 atgaaagtcg aaaccgttgt tagcggcgat ctggtccaga tcccgtcgca caggaacgtt
    14821 gtgtctcagc atgattagcc tccccaatgc ccacgggcgc tggaagaacg accatagccg
    14881 ccgcgcgaaa tggttgacgc ttttttggtg gtaaagccgc ctgaagagta agactctttt
    14941 ttgccagaac cggagcgcca ggagtaatca ccggaagtgt tgagccagac ggggcgggaa
    15001 gcaaatgagg atccgccgct gttatagacg aacggctcat cgcgatcttt acgaataacc
    15061 cggcttaaca aaaatccaga aacgaccggg atccaactct gttcaacatt gtcgtagtag
    15121 caattttcgt acttagactg acaattctgc tgagtcatat tcttcggaac atcggcataa
    15181 aatgccgctt tggcgttgtt ccagccacgc gcgcaaatgt cggcattatt accgtcatca
    15241 atacaatcct gcactgtcga ataaaacgtt ccgtcgccgt cgttatcgac atcgccacta
    15301 tcgccacaac ctttcaatac aaaaaaagcg gcaccgccca ttagcgccaa cgttaagtat
    15361 ttttgtgcgt agcgattgcg acgcggttca aaaggattat tcggtctacc aatgcgactg
    15421 atcgctccgt gaccaatttt actgttattt cttgatttgc gttttctggc catgatcgtc
    15481 cattaccatg tcatacaagc tgcgttaaaa ataccgccag ccagcgcggc ggttcccata
    15541 aacattcctg ctgcggtatt gtgattaata attttttcgc ttaatgcggg catataaagt
    15601 ctgacaccag caaaaacgag taactgaatc accagtgcaa tcccgcccca ggcgaaatag
    15661 tctggaatac tcaccgcatt gattgccgca ctggataagg ggatcacgta acccaacaat
    15721 gtaccgctga atgccagtga caccgcggta ttattgtttt tgattaattg ccattcgttg
    15781 tgcggtgtaa ttttggagta gataaacaga aaaataatca ccatagccac gccaataaaa
    15841 aaataggcac taaaggcaag aagtgaatcc agtatatgca ttgtaaacgt ccttgtaatt
    15901 aaccaataat atgaagtgat gtcagcggga tatcgacacc caacgcgcgc gaaaataacc
    15961 actctggctc gccgagatcg ttaaaagatt cctcaccatt taacagtaaa tattcgtaag
    16021 tatcttcggt tacttgccgc tggtagccca tggtaaaatt atggacctcc catttggcat
    16081 ggttttgatt ttctactttt tccagcatgt agacgggttc gatatttccc ggttcttcgc
    16141 tgttaaaaaa tcgctgccag cgtttttcct gccagtttaa ggtcattgcc cccatcgttt
    16201 tggcgttgat cgcctcgcgc cagtgactct ctttactgat accgtagctc tcttcataga
    16261 caaagagctt gatatcatca atgtcatcaa tatcttcgcc gccggtggta ttgatttgca
    16321 gaaattcatc gcctgaagtg tagtaacgga aaatctggct accgcctccc agatcgatgc
    16381 ggctgacagc ggctaccgta aattcctcac ccggtagcgc aatcagcaat tcatcttcca
    16441 gcaaacgaaa cgctaacgtg tcgagcgtaa agccactatt gagatgaagt cccagcggac
    16501 cgcgagcgat agcaggctta ttatccttgc caaacagacg ctggaaaaaa ccagacatat
    16561 tttatcctta aaatagttgc cagcctttta gcgaggctgg caaaaaaatt actcgccctg
    16621 ttggcgttgc agtcttgcta atacatcctg ggctctactt ttattgctac cgccaattcc
    16681 ggcttccgcc agcttttcgt caaggtcgcg accgtctgcg actttctcca actgtgcggc
    16741 agcatccagg cgagcctgac gttcggcctg acgcgtttgc aggcgtttta aggattctgc
    16801 cgctgtcgaa acgctggaag atgcgccaac ggtagaggtt gttacagcct gttgtgcacg
    16861 ctgcatggct tcagtggctt taacgacttc catttgctgc tcaaactgag cgatacgctg
    16921 cgctgtcgct gtaactgctt tttccacgcc atcacgggag acttccagat tcgacaacac
    16981 ttgctcttca gcggtaatga gattctcaag gcgcgcgatt tcttcagcaa cttcgttaat
    17041 caacgacgga ttaacgttct tgcttaacgc ttccagcgca cgggcttcca gactggcttt
    17101 gcgctcacgt aaatctttca gcttatcgtg acttaatttc acccgcgcca gcagatcaac
    17161 gcgagatttt ccggctttat cgagttcagc tttagcgtca cgaatatgct gttccagcat
    17221 gcgcacgccc tgggtttctt caatggattc ttccgcctgg gagataaacg atttccccag
    17281 cgtaaataaa ctttttaaaa ttcccataaa tacccgtcct tgtaattagt gtgaatattc
    17341 ttctgtaatt tcagccagat ccaatgcgtt atctaccagc taggttatct caagcaggat
    17401 atcttcaaga gaagatttaa gcgataacgc cccgaaaaca atgtaatact cttcctgttg
    17461 cacgctggag attcctaccg atgacaacgg catcatcttc t
//
LOCUS       DAJTMC010000080        17483 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.080, whole genome shotgun sequence.
ACCESSION   DAJTMC010000080 DAJTMC010000000
VERSION     DAJTMC010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17483
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.080"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(34..2631)
                     /gene="topA"
                     /locus_tag="OO130_002384"
     CDS             complement(34..2631)
                     /gene="topA"
                     /locus_tag="OO130_002384"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HCR5365766.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVNKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            3011..3262
                     /locus_tag="OO130_002385"
     CDS             3011..3262
                     /locus_tag="OO130_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HCR5365767.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            complement(3298..4347)
                     /gene="sohB"
                     /locus_tag="OO130_002386"
     CDS             complement(3298..4347)
                     /gene="sohB"
                     /locus_tag="OO130_002386"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HCR5365768.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMGSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKY
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            4567..5325
                     /locus_tag="OO130_002387"
     CDS             4567..5325
                     /locus_tag="OO130_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HCR5365769.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDICPMSEQNPQVWQDVMQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            5322..5913
                     /gene="cobO"
                     /locus_tag="OO130_002388"
                     /pseudo
     CDS             5322..5913
                     /gene="cobO"
                     /locus_tag="OO130_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
     gene            complement(5953..6825)
                     /gene="rluB"
                     /locus_tag="OO130_002389"
     CDS             complement(5953..6825)
                     /gene="rluB"
                     /locus_tag="OO130_002389"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HCR5365770.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNG"
     gene            complement(7036..8931)
                     /locus_tag="OO130_002390"
     CDS             complement(7036..8931)
                     /locus_tag="OO130_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HCR5365771.1"
                     /translation="MAGKFRCILLLIAGLFVSSLSYAENPEIPSYEEGISLFDVEATL
                     QPDGVLDIKENIHFQARNQQIKHGFYRDLPRLWMQPDGDAALLNYHIVGVTRDGIPEP
                     WHLDWHIGLMSIVVGDKQRFLPQGDYHYQIHYQVKNAFLREGDSDLLIWNVTGNHWPF
                     EIYKTRFSLKFPDIAGNPFSEIDLFTGEEGDTYRNGRILEDGRIESRDPFYREDFTVL
                     YRWPHALLSNAPAPQTTNIFSHLLLPSTSSLLIWFPCLFLVCGWLYLWKRRPQFTPVD
                     VIETDVIPPDYTPGMLRLDAKLVYDDKGFCADIVNLIVKGKIHLEDQYDKNQQILICV
                     NEGATRNNEVLLPAEQLLLEALFRKGDKVVLTGRRNRVLRRAFLRMQKFYLPRKKSLF
                     YRPDTFLQWGGLAILAIILYGNLSPVGWAGMSLVGDMFIMICWIIPFLFCSLELLFAR
                     DDDKPCVNRAIITLFLPLICSGVAFYSLYINVGDVFFYWYMPAGYFSAVCLTGYLTGM
                     GYIFLPKFTQTGQQRYARGEAIVNYLARKEAATHSGRRRKGETRKLDYALLGWAISAN
                     LGREWALRIAPSLSSAIRAPEIARNGVLFSLQTHLSCGANTSLLGRSYSGGGAGGGAG
                     GGGGGGW"
     gene            complement(8959..9579)
                     /locus_tag="OO130_002391"
     CDS             complement(8959..9579)
                     /locus_tag="OO130_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HCR5365772.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            complement(9576..10457)
                     /gene="yciV"
                     /locus_tag="OO130_002392"
     CDS             complement(9576..10457)
                     /gene="yciV"
                     /locus_tag="OO130_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HCR5365773.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQQIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLTTLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            10595..10639
                     /gene="trpL"
                     /locus_tag="OO130_002393"
     CDS             10595..10639
                     /gene="trpL"
                     /locus_tag="OO130_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HCR5365774.1"
                     /translation="MKAIFVLKAWWRTS"
     misc_feature    10616..10709
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            10731..12293
                     /gene="trpE"
                     /locus_tag="OO130_002394"
     CDS             10731..12293
                     /gene="trpE"
                     /locus_tag="OO130_002394"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HCR5365775.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGDRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQALSGNGEALLTLLDNALPAGVENEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKNTRIQASLFAPNEEEKQRLTARLNDLRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRTGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGIVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     gene            12293..13888
                     /gene="trpD"
                     /locus_tag="OO130_002395"
     CDS             12293..13888
                     /gene="trpD"
                     /locus_tag="OO130_002395"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HCR5365776.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQRKLEPTNTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            13889..15250
                     /gene="trpCF"
                     /locus_tag="OO130_002396"
     CDS             13889..15250
                     /gene="trpCF"
                     /locus_tag="OO130_002396"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HCR5365777.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFNPARIAAIYKHYASAISVLTDEKYFQGSFDFLP
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMG
                     GLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPTHVAIWKALSVGETLPAREFQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            15262..16455
                     /gene="trpB"
                     /locus_tag="OO130_002397"
     CDS             15262..16455
                     /gene="trpB"
                     /locus_tag="OO130_002397"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HCR5365778.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVCEFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRETPEKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            16455..17261
                     /gene="trpA"
                     /locus_tag="OO130_002398"
     CDS             16455..17261
                     /gene="trpA"
                     /locus_tag="OO130_002398"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HCR5365779.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLRDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
BASE COUNT         4220 a         4673 c         4345 g         4245 t
ORIGIN      
        1 cctttgttta taaaaacctg acagaattaa aggttatttt tttccttcaa cccatttgcc
       61 atcaacataa aatgctgacc agccagtcgc ctttccgtct ttttccgaag agacatattg
      121 ctgtttggtt ttacggctaa agcgaaccat ggtcttatta ccttccggat cctgctgtgg
      181 cgcatcggcc agataacgca gtttttccgg cagacggtcg cggaagcgat aaagctcttc
      241 caccagtggc gcacgcgttt cacgcgattt cgggaaagtg ttggcagcca ggaaaacacc
      301 cgcagcgccg tcacgcagca cgaaataagc atctgatttt tcgcacggca gctcaggtaa
      361 tggcaccgga tcttctttcg gtggtgccac ttcgccgtta cgtaaaatct tacgtgtgtt
      421 tttacactct tcgttggtgc aggccatgta tttaccgaaa cgccccattt tcaggtgcat
      481 ttcagagcca catttttcac actcaacgat cgggccgtca taacctttaa tgcggaattc
      541 gccctcttcg atctcgtaac cgtcgcaggt tgggttatta ccacagacat gcaacttacg
      601 tttcggatcg atgagatagc tgtccatcgc cgtgccgcat ttcgggcaac gacgttttgc
      661 gcgcagcgcg ttggtttcag cgtcttcgcc ttccagtacg ttcagcactt cgttttccgg
      721 caccaggtta atggtggttt tgcaacgctc tttcggcggc agcgcataac cagaacagcc
      781 aaggaatacc ccggtgctcg ctgtgcgaat ccccattttg cgaccacaag tcgggcagtc
      841 aatgctggtc agaaccatct ggttcgggcg cataccaccc tcttccggat ctttttcagc
      901 tttatctaac tgctgggtga aatccgagaa gaagtgatcc agtacagctt tccactctgc
      961 ttcgtgattt gccacctggt cgaggctgtt ttccatctgt gcggtgaagt cgtagttcat
     1021 taactcgcgg aaattttctt caaggcgatc ggtgacgatt tcgcccattt tttccgcata
     1081 gaaacgacga ttttctactc gcacgtagcc acgatcctga atggtcgaaa tgatcgacgc
     1141 ataggtagac ggacgaccga taccgcgttt ttccagctct ttaaccagcg atgcttcact
     1201 gaaacgggct ggcggcttgg taaagtgctg ggctggtgta agttcaacga gcgtcagagc
     1261 atcgccttta ttaactgctg gtaagatgcg atcttcatcg cctttacgca acgcaggcat
     1321 cacttttgtc cagccatcaa aacgcaaaat acgacctcgt gctttcaggc ggaaatcgcc
     1381 cgcaccaacg gtcagcgtcg tggagtcata tttcgctggg gtcatctggc aggctacgaa
     1441 ctgacgccag attaactggt acagtttctg cgcatctgct tccatatcct tcagcgattc
     1501 cgccatcaca ttgacgtcag aaggacgaat cgcttcgtgc gcttcctgtg agttttcttt
     1561 gctggcgtac tgattcggac tttccggcag atatttctta ccaaaattat cgctgatata
     1621 accgcgaacc atatttaccg cgtcctgact caggttagtg gagtcggtac gcatgtaagt
     1681 gatatagcct gcttcataca aacgctgcgc catcatcatg gtttttttca cgccaaatcc
     1741 aagacgggtg ctggcagctt gttgcagcgt agaggtaata aaaggagcgc caggtttact
     1801 ggttgtcggt ttgtcttcac gttccagcac gctgtagcgc gctttttcca gcagacttac
     1861 cgcagcctga gtttgttctt tgttgaccgg acggaacggt ttgtcgttct gatgagtcac
     1921 ctgtaacgcc aacgcttcac cagatggcgt ggtcgtgctg gcatcgactt cccagaactc
     1981 ttccggcacg aacgctttaa tttcacgctc acgctcgacc accaggcgaa ccgccaccga
     2041 ctgcacacga ccggcagaca gaccacgagc gatctttttc catagcagcg gcgaaaccat
     2101 ataccccacc acgcggtcca taaagcgacg cgcctgctgg gcattaacac gatcaatatt
     2161 cagctcaccc ggtttgttaa atgcctggcg gatcgcgttt ttagtaattt cgttaaacac
     2221 cactcggcta tagcgcgcat catcaccccc aatcacttcc cgcaggtgcc atgcaatggc
     2281 ttccccttcg cggtcaaggt cggttgcgag atagatgtgg tcggcttttt cagccagttg
     2341 tttcagttca gagacgacct tctctttacc aggcaacact tcatagtgcg cctcccaatt
     2401 gtgccacggg tcaaccccca tacggttgac gagagcgcca cgttcatcct ttttaggctt
     2461 tttagccgtc ttggtggagg tagagtcggc actctttttg gcagctgagc cactggtcgg
     2521 caaatcgcgg atgtgaccga cgctggattt caccacgtag tcactaccca gatacttgtt
     2581 gatcgttttg gcttttgccg gggactcaac gatgacaaga gctttaccca tattcacctt
     2641 acctaatttg attcttccag gaatgcgtcg cacgttgata aaccttccac tggcaacgag
     2701 ttaccgatat ggactcgttc tctgcggata tcaacccctt gtcgtgactc tgttcacgaa
     2761 aattcaggtg actgagttaa caggtcttgg cctacaggcg ctatagcacg acggaatttt
     2821 ggtcgaatgt caagcaaatc tattgccaga ttgacgaaag cgtcacactg tacctgataa
     2881 aatccgatac gcaactttat tagcatgcaa aatccaaagt tgccagtaag gcgctcttgc
     2941 caccagcgtg gtatatttgt ctgcaagcca tgcctggcgt agactaatgt ccttatttaa
     3001 ggagtaaata atgaacaaag agacacaacc catcgaccgc gaaacgctgt tgaaagaggc
     3061 caacaaaatc attcgtgagc atgaggatac gctcgcaggg attgaagcca ccggcgtcac
     3121 gcagcgtaat ggcgtgctgg tatttaccgg cgactacttt ttagatgaac aaggattgcc
     3181 aacagccaaa agcaccgcgg tgtttaatat gttcaaacac ctggcccatg tgctttcaga
     3241 aaaatatcat ctggttgatt aaagcaaaac gcgaggtctt agcctcgcgt ttgtctttta
     3301 catcaatggc ttttgacccc gctgccacca gcgtagcaac aatcgatcgg cgctctctgc
     3361 cgcgctgccg gtgaatcggt caatgagtcg tttacgctgc atatagcgta cattaaccac
     3421 ttcacggcct tccatcaggc taagaataac ttcatcactg gtgttgattt catcaaccag
     3481 gcctttctct accgcctgtt gtccgtacca gtgttcacct gttgccacct gttcaatatc
     3541 cagagacgga cgcatacgct tcacaaaatc tttaaacaac tgatgcgttt cgttcaactc
     3601 ttcgcggaat ttctcccgcc cttcttcggt attttcaccc agcaacgtca gagtacgctt
     3661 atactgcccg gcagtgtgca gttcgatatc aatatatttg cttttcagga agcggttaaa
     3721 gttgggcatt tgcgccacca ccccaatgga acccacaata gcaaacggtg cggaaacaat
     3781 tttgtccgcc acacaggcca tcatgtaacc gccgctggca gcgactttgt ctaccgtaac
     3841 agttaaagga atgtttttat cacgcagacg ctgcagctgc gaagccgcca acccgtaacc
     3901 atgcaccatg ccaccagggc tttccagacg gagcacaacc tgatcctgcg gtttgaatgc
     3961 tgcgagtacc gccgttatct cttcacgtag cgagttcact tcatgggcgt ccatgctgcc
     4021 tttaaaatcc agcacccaga cgcgaggttt actgtcagtt gccacctcgc ccagtttggc
     4081 tttcgctttt gctgctttcg cttcttgctt gtgctttttc ttctgcgctt tgtgccactg
     4141 tttttgctga tgtgagccca tcagcgccgc ggccagttct tctttcatct ccttatactg
     4201 ttcgctgaga ttgttgaccc gtaactcgcc acgctggcgt ttattacgtt gagcaacatt
     4261 gacaataatg gcggcaatcg ccgcaatcgc tagcacaacg gtaacgattt tcgccaaaaa
     4321 caaaccatat tcagacaaca attccacgag tccccacctt ggttaaacaa cgctttggct
     4381 cacagtttac aacaggcatg ttaagccgtc tcgcagagat gttattgcag cgaaagtatg
     4441 acaaagtatc cattttgcgg tgttatcgcc ccaggctgat tgaaatgccc ttctgtttca
     4501 ggcataaagc cccaaagtca taaagtacac tggcagcgcg acagacaacg tcgttgagga
     4561 gtagccatgc attaccagcc aaaacaagat ttactcaatg atcgcattat cctggtgacg
     4621 ggagccagcg atgggattgg tcgtgaagcc gcgatgacgt atgcacgcta tggtgcgaca
     4681 gtgattctgt tgggccgtaa tgaagaaaaa ttacgtcagg tagccagcca cataaacgaa
     4741 gaaactgggc gtcagccaca gtggtttatt ctcgatttgc tgacctgcac gtccgaaaat
     4801 tgccaacaac tggcacagcg cattgtcgtt aattatccgc gtctggatgg tgttttgcat
     4861 aatgccggat tgctcggcga tatttgccca atgagcgaac aaaatccgca ggtctggcag
     4921 gacgtcatgc aggtcaacgt taatgccacc tttatgctca cccaggcact gcttccttta
     4981 ttactcaaat cggacgccgg ttcactggtc tttacttcat caagcgttgg acgtcaggga
     5041 cgagctaact ggggtgcata tgcagcgtcg aaatttgcca ccgaagggat gatgcaggta
     5101 ctggccgatg aatatcagca gcgcctgcgc gtcaactgca ttaacccagg cggtacgcgc
     5161 accgcaatgc gcgcaagcgc cttcccgacc gaagatccac agaaacttaa aacacccgct
     5221 gatatcatgc cgctctacct ctggctgatg ggcgatgaca gccgccgtaa aaccggcatg
     5281 acctttgacg cccaaccggg ccgtaaacca ggaatttccc aatgagtgat gaacgctacc
     5341 aacagcgtca gcagcgagtg aaagaaaaag tagatgctcg tgtggcacag gccccaggat
     5401 gaacgcggta ttatcatcgt ctttaccggc aatggaaaag gcaaaaccac cgcggcattt
     5461 ggtacggcaa cacgcgcagt tggtcacgga aaaaaagtgg gcgtcgtgca gtttattaaa
     5521 ggcacctggc ctaatggcga acgcaatctg ctggaaccac atggcgttga gtttcaggtg
     5581 atggcaacgg gctttacctg ggatacacaa aaccgcgagt ctgataccgc cgcctgccgc
     5641 gaagtctggc aacatgcaaa gcggatgctt gctgattcct cactggatat ggttttgctt
     5701 gatgaactga cgtatatggt ggcgtatgac tatttgccac tggaagaagt ggtgcaggcg
     5761 ttaaatgaac gtccacatca acagacggtg attatcacgg gtcgaggctg tcatcgggat
     5821 attcttgaac tggcggatac ggtaagtgag ctgcgcccca tcaaacatgc gtttgatgcc
     5881 ggtgttaagg cgcaaatagg gattgattat taatctgtaa aaatgccgga tatgacgtcc
     5941 ggcatacttt gattaaccgt tattacgccc gccagaacga cgaccgccgc tcacctgact
     6001 gtgacgtttc accgcacgac gaatctgatt agccttcatg cgacgacggt ctttttctac
     6061 agcgacttta gagctggttt ctggcggaag ctccaccagt tcgcgcagat agttagtctg
     6121 ggcgaggtcc agctccgtcc agccaccgcg tggcagacct tttggcagtg ggatatcacc
     6181 gtaacgaaca cggatcaggc ggctcacctg cacaccaacc gcttcccaca gacgacgaac
     6241 ttcacggtta cggccttcgg tcagagtcac gttgtaccac tggttgatcc cttcgccgcc
     6301 gctgaacttg atggttttaa aagctgccgg accatcttcc agttgcacgc cacgactcaa
     6361 atcacgcagt ttcgcgtcgt caacctgacc aaatacacgc acggcgtatt cacgttcaac
     6421 ttcacggctt ggatgcatta aacggtttgc cagttcacca tcggtggtga acagcagcag
     6481 accacaggtg ttaacgtcca gacgacctac ggcaatccag cgtgcgccac gcagctttgg
     6541 cagacggtca aacaccgtcg gacgcccttc cggatcgtta cgggtacaca actcaccttc
     6601 cggtttgtaa taggccagaa cgcgacaaat ttgttcagcg gactcacgta ccgaaatcag
     6661 atgaccatcg atacgaattt tcagcccagg ggtaacttca acgcgatcgc cgagtttagc
     6721 aattttgcca tcaacactca cacgaccggc ttcaataata gattcgattt cacggcggga
     6781 gccgtggcca gcgcgggcca gcactttctg taatttttcg ctcatagagc ttccttttgg
     6841 tgtcgccttc ccaggcgtct gatatgcttt tgtgcaacag tgagtcagaa cttacatctg
     6901 actcactgat agtaaagtct gtttgaactc ttcaggcctt aagtaaacga ctcgacgttt
     6961 atcggcctgg ctttgaggcg caacatacta cactatcttt atggttaaag cttgtttcga
     7021 ttaacatcag cttaattacc agccaccacc gcctccgcca cccgcgccgc cgccagcacc
     7081 accaccggaa taacttcgcc ccaacaaact ggtattggcc ccgcaactta ggtgcgtctg
     7141 taatgagaat aaaacgccgt tacgggcaat ctctggagcg cgaatcgccg aagaaagcga
     7201 aggggcaatg cgtaacgccc attccctccc caaatttgcc gatatagccc aacctagcaa
     7261 cgcgtaatcc agtttccgtg tttccccttt ccgccgacgt ccactgtgtg ttgctgcctc
     7321 tttacgcgca agatagttaa cgatagcttc accgcgggca taacgttgct gcccagtttg
     7381 ggtaaacttt ggcagaaaaa tataccccat gccagtgaga taaccggtca ggcaaacagc
     7441 gctaaaataa cccgctggca tataccagta aaagaataca tctccgacat tgatatagag
     7501 agaatagaag gccacgcctg aacaaatcag tggtaaaaac aaagtgatga ttgcacgatt
     7561 aacgcaaggc ttgtcatcat cgcgggcaaa caaaagctca agggaacaaa ataaaaaagg
     7621 aataatccag cagatcataa taaacatatc gcctaccaga ctcattcctg cccaacctac
     7681 gggactcagg ttaccgtaga gaatgatcgc caatattgcc agtccacccc attgcaaaaa
     7741 cgtatcaggc cggtaaaaca aagacttttt acgcggcaga taaaatttct gcatccgtaa
     7801 aaatgccctg cgtaagactc tgttgcgtct ccccgtaaga acgaccttat cgcctttacg
     7861 aaataacgct tccagtaata actgctctgc gggcagtaat acctcattat ttctggtcgc
     7921 gccttcatta acacaaatca ggatttgctg gttcttatca tactgatctt ccagatgaat
     7981 ttttcccttc acgatcagat ttacgatatc ggcacagaaa cctttatcgt cgtaaaccag
     8041 cttcgcatcg agacgtaaca tgccgggcgt gtaatctggc ggaatgacat cggtttcaat
     8101 cacatctacc ggcgtaaatt gcggcctgcg cttccagaga tataaccatc cacaaaccag
     8161 gaatagacac ggaaaccaaa ttaacaacga tgacgtggag ggtaaaagaa gatggctgaa
     8221 aatattcgtc gtttgtggag ccggggcatt gctaagtaac gcgtgaggcc agcggtagag
     8281 taccgtgaaa tcttcacgat aaaacggatc gcgggattct attcttccgt cctcaaggat
     8341 gcggccattt cgatatgtgt cgccctcttc tccagtaaag agatcgattt cgctaaatgg
     8401 attacccgcg atatcaggga acttgagtga aaatcgggtc ttatagattt caaacggcca
     8461 gtggttacca gtcacgttcc agattaacag atctgaatct ccctcacgca ggaaagcatt
     8521 tttaacctga taatgaattt gataatgata gtcgccttga ggcaagaaac gttgtttatc
     8581 gcccacgaca atactcatta acccgatatg ccagtcaaga tgccagggtt caggaatacc
     8641 atcacgagtg acgccaacaa tatgatagtt cagcagtgca gcgtccccat caggctgcat
     8701 ccagagtcgt ggtaaatcac gataaaatcc gtgcttaatc tgctgatttc gcgcctgaaa
     8761 atgaatattt tctttgatgt cgagcacccc atccggttgc agagtggctt caacatcaaa
     8821 gagcgagatc ccttcttcat aagaagggat ctccgggttt tctgcataac ttagagatga
     8881 tacaaacagc cctgctatca acagcaaaat gcagcgaaac ttccctgcca tagtcttcca
     8941 tccgtggaac gcaatttgtc ataagaaagg cttcacatca cccacgcctt cacgcaccac
     9001 gaccggcgta tcatcggtaa gatcaatgac cgttgtcggt ttctgtccga gatagccgcc
     9061 gtggataatc aaatccacct gtttttccag acggtcttta atttcttctg gatcggattc
     9121 ggtaaattcg ctgcctggca gcattagcga agtggaaagc atcggttcgc ccagtgcttc
     9181 aagcaacgcc tgcgcaatcg ggttagaagg cacacgcata ccgatggttt tgcgtttttc
     9241 ctgcaacaga cggcgtggca cctccttcgt ccccttcagg atgaaggtat agttgcccgg
     9301 cgtgttgttt ttcattaaac gaaacgcaac gttatccacg aatgaatagg tcgaaagttc
     9361 agaaagatcg cgacacatca gggtaaagtt gtgaccgtcc ggcagctggc gaatacgaca
     9421 aatacgctcc atcgcgtttt tatcttcaat tttacagccg agcgcatagc cggaatcagt
     9481 tggataaaca atcaccccgc ctttacgcac gatctccacc gcctggttga tcagacgttg
     9541 ctgcgggtta tcaggatgaa tataaaaaaa ctggctcata attccctctc tgtggtgttc
     9601 tgcggctgtt cccatagctg ccagacgcct tcaacgcctg cgggcagcca gagtttacga
     9661 cccagttcga tccacgggca tggctgatga aaatcagatc cttgtgatgc ccataaatga
     9721 tgctgacgcg caagtgtcgt caactgggtg cgttcattgg gcgattgctg gcactgcgcg
     9781 acttccatcg cgtcgccgtg gtgttcggca aaatgcgcaa ccaatctttt cagccattta
     9841 gcactaagat tgtaccgccc aggatgagct agcaccgcct taccgccaga atgatgaatg
     9901 acatcaatag cttgttctat tgtacaccac tgtggcggaa cgtatccggt tttcccgcgc
     9961 gccagatact ttttaaagac atctgccatt gaactggctt tgccgcactc aacaaggaaa
    10021 cgcgcaaaat gaccgcgcgt cactgcgccg ccctgcgcca gtcgttgtgc gccttccagc
    10081 gccccaggta tttgcgcttt ttcaagtctt tcggcaattt gctgcgcccg ctgattgcgc
    10141 cgttctgtct gctgcgcgag gaactcacac attagcggat gagtaatatc aatattcagc
    10201 ccgacaatat gaatttcatg attttcccag accgtggaaa tttccacgcc gggaataaga
    10261 ttcagcgcca gtcccgaacg tgaaatttcc tctcttgctg gcgcgattgc agctgtggtg
    10321 tcatggtcgg tgatcgccag ggtgccgacg cgcatctcga ctgcacggtg caccaatgct
    10381 tctggcgtca ggcagccatc ggaagctgtg gtatggctgt gcaggtcgta aatcactgcg
    10441 taattcgtgt cgctcaaggc gcactcccgt tctggataat gttttttgcg ccgacatcat
    10501 aacggttctg gcaaatattc tgaaatcagc tgttgacaat taatcatcga actagctaac
    10561 tagtacgcaa gttcacgtaa aaagggtatc gacaatgaaa gcaattttcg tactgaaagc
    10621 ttggtggcgc acttcctgaa acgggcagtg tattcaccat gcgtaaagca atcagatacc
    10681 cagcccgcct aatgagcggg cttttttttg aacaaaatta gagaataaca atgcaaacac
    10741 aaaaaccgac tctcgaactg ctaacctgcg aaggcgctta tcgcgacaac ccgactgcgc
    10801 tttttcacca gttgtgtggg gatcgtccgg caacgctgct gctggaatcc gcagatatcg
    10861 acagcaaaga tgatttaaaa agcctgctac tggtagacag tgcgctgcgc attacagcat
    10921 taggtgacac tgtcacaatt caggcacttt ccggcaacgg cgaagccctg ctgacactac
    10981 tggataacgc cctgcctgcg ggtgtggaaa atgaacaatc accaaactgc cgtgtgctgc
    11041 gcttcccccc tgtcagtcca ctgctggatg aagacgctcg cttatgctcc ctttcggttt
    11101 ttgacgcttt ccgtttattg cagaatctgt tgaatgtacc gaaggaagaa cgagaagcaa
    11161 tgttcttcgg cggcctgttc tcttatgacc ttgtggcggg atttgaagat ttaccgcaac
    11221 tgtcagcgga aaataactgc cctgatttct gtttttatct cgctgaaacg ctgatggtga
    11281 ttgaccatca gaaaaaaaac acccgcattc aggccagcct gtttgctccg aatgaagaag
    11341 aaaaacaacg tctcactgct cgcctgaacg atcttcgcca gcagctgacc gaagccgcgc
    11401 cgccgctgcc ggtggtttcc gtgccgcata tgcgttgtga atgtaaccag agcgatgaag
    11461 agttcggtgg cgtggtgcgt ttgttgcaaa aagcgattcg caccggagaa attttccagg
    11521 tggtgccgtc tcgccgtttc tctctgccct gcccgtcacc gctggcggcc tattacgtgc
    11581 tgaaaaagag taatcccagc ccgtacatgt tttttatgca ggataatgat ttcaccctgt
    11641 ttggcgcgtc gccggaaagt tcgctcaagt atgacgccac cagccgccag attgagatct
    11701 acccgattgc cggaacacgc ccgcgcggtc gtcgcgccga tggttcactg gacagagacc
    11761 tcgacagccg catcgaactg gaaatgcgta ccgatcataa agagctttct gaacatctga
    11821 tgctggtgga tctcgcccgt aatgatctgg cacgcatttg cacccccggc agccgctacg
    11881 tcgccgatct taccaaagtt gaccgttact cttacgtgat gcacctggtc tcccgcgtgg
    11941 tcggtgagct gcgccacgat ctcgacgccc tgcacgctta ccgcgcctgt atgaatatgg
    12001 ggacgttaag cggtgcgccg aaagtacgcg ccatgcagtt aattgccgag gcggaaggtc
    12061 gtcgccgcgg cagctacggc ggcgcggtag gttatttcac cgcgcatggc gatctcgaca
    12121 cctgcattgt gatccgctcg gcgctggtgg aaaacggtat cgccaccgtg caagccggtg
    12181 ctggcatagt ccttgattct gttccgcagt cggaagccga cgaaacccgt aataaagccc
    12241 gcgctgtact gcgcgctatt gccaccgcgc atcatgcaca ggagactttc tgatggctga
    12301 cattctgctg ctcgataata tcgactcttt tacgtacaac ctggcagatc agttgcgcag
    12361 caatgggcat aatgtggtga tttaccgcaa ccatattccg gcgcagacct taattgaacg
    12421 cctggcgacg atgagcaatc cggtgctgat gctttctcct ggccccggtg tgccgagcga
    12481 agccggttgt atgccggaac tcctcacccg cttgcgtggc aagctgccca ttattggcat
    12541 ttgcctcgga catcaggcga ttgtcgaagc ttacgggggc tatgtcggtc aggcgggcga
    12601 aattctccac ggtaaagcct ccagcattga acacgacggt caggcgatgt ttgccggatt
    12661 aacaaacccg ctgccagtgg cgcgttatca ctcgctggtt ggcagtaaca ttccggccgg
    12721 tttaaccatc aacgcccatt ttaatggcat ggtgatggca gtacgtcacg atgcggatcg
    12781 cgtttgtgga ttccagttcc atccggaatc cattcttact acccagggcg ctcgcctgct
    12841 ggaacaaacg ctggcctggg cgcagcggaa actagagcca accaacacgc tgcaaccgat
    12901 tctggaaaaa ctgtatcagg cgcagacgct tagccagcaa gaaagccacc agctgttttc
    12961 agcagtggta cgtggcgaac tgaaaccgga acaactggcg gcggcgctgg tgagcatgaa
    13021 aattcgcggc gagcacccga acgagatcgc cggagcagca accgcgttac tggaaaacgc
    13081 cgcgccgttc ccgcgcccgg attatctgtt tgccgatatc gtcggcaccg gtggtgacgg
    13141 cagtaacagc atcaatattt ccaccgccag tgcgtttgtc gccgcggcct gtgggctgaa
    13201 agtggcgaaa cacggcaacc gtagcgtctc cagtaaatct ggttcgtccg atctgctggc
    13261 ggcgttcggt attaatcttg atatgaatgc cgataaatcg cgccaagcgc tggatgagtt
    13321 aggcgtatgt ttcctctttg cgccgaaata tcacaccgga tttcgccatg cgatgccggt
    13381 tcgccagcaa ctgaaaaccc gcactctgtt caacgtgctg ggaccattga ttaacccggc
    13441 gcatccgccg ctggcgttaa ttggtgttta tagtccggaa ctggtgctgc cgattgccga
    13501 aaccttgcgc gtgctggggt atcaacgcgc ggcggtagtg cacagcggcg gtatggatga
    13561 agtttcatta cacgcgccga caatcgttgc cgaactgcat gacggtgaaa ttaagagcta
    13621 tcagctaact gcagaagatt ttggcctgac accctaccac caggagcaac tggcaggcgg
    13681 aacaccggaa gaaaaccgtg acattttaac acgcttgtta caaggtaaag gcgacgccgc
    13741 tcacgaagca gccgtcgcgg cgaatgtcgc catgttaatg cgcctgcatg gccatgaaga
    13801 tctgcaagcc aatgcgcaaa ccgttcttga ggtactgcgc agtggttccg cttacgacag
    13861 agttaccgca ctggcggcac gagggtaaat gatgcaaacc gttttagcga aaatcgtcgc
    13921 agacaaggcg atttgggtag aaacccgcaa acagcagcaa ccgctggcca gttttcagaa
    13981 tgaggttcag ccgagcacgc gacattttta tgatgcgcta cagggtgcac gcacggcgtt
    14041 tattctggag tgcaagaaag cgtcgccgtc aaaaggcgtg atccgtgatg atttcaatcc
    14101 ggcacgcatt gccgccattt ataaacatta cgcttcggca atttcggtgc tgactgatga
    14161 gaaatatttt caggggagct ttgatttcct ccccatcgtc agccaaatcg ccccgcagcc
    14221 gattttatgt aaagacttca ttatcgaccc ttaccagatc tatctggcgc gctattacca
    14281 ggccgatgcc tgcttattaa tgctttcagt actggatgac gaccaatatc gccagcttgc
    14341 agccgtcgct cacagtctgg agatgggggg gctgaccgaa gtcagtaatg aagaggaact
    14401 ggagcgcgcc attgcattag gggcaaaggt cgttggcatc aacaaccgcg atctgcgtga
    14461 tttgtcgatt gatctcaacc gtacccgcga gcttgcgccg aaactggggc acaacgtaac
    14521 ggtaatcagc gaatccggca tcaatactta cgctcaggtg cgcgagttaa gccacttcgc
    14581 taacggtttt ctgattggtt cggcgttgat ggcccatgac gatttgcacg ccgccgtgcg
    14641 ccgggtgttg ctgggtgaga ataaagtatg tggcctgacg cgtgggcaag atgctaaagc
    14701 agcttatgac gcgggcgcga tttacggtgg gttgattttt gtcgcgacat caccgcgttg
    14761 cgtcaacgtt gaacaggcgc aggaagtgat ggctgcggca ccgttgcagt atgttggcgt
    14821 gttccgcaat cacgatattg ccgatgtggt ggacaaagct aaggtgttat cgctggcggc
    14881 agtgcaactg catggtaatg aagatcagct gtatatcgat acgctgcgtg aagctctgcc
    14941 aacacatgtt gccatctgga aagcattaag cgtcggtgaa accctgcccg cccgcgagtt
    15001 tcagcacgtt gataaatatg ttttagacaa cggccagggt ggaagcgggc aacgttttga
    15061 ctggtcacta ttaaatggtc aatcgcttgg caacgttctg ctggcggggg gcttaggcgc
    15121 agataactgc gtggaagcgg cacaaaccgg ctgcgccgga cttgatttta attctgctgt
    15181 agagtcgcaa ccgggcatca aagacgcacg tcttttggcc tcggttttcc agacgctgcg
    15241 cgcatattaa ggaaaggaac aatgacaaca ttacttaacc cctattttgg tgagtttggc
    15301 ggcatgtacg tgccacaaat cctgatgcct gctctgcgcc agctggaaga agcttttgtc
    15361 agcgcgcaaa aagatcctga atttcaggct cagttcaacg acctgctgaa aaactatgcc
    15421 gggcgtccaa ccgcgctgac caaatgccag aacattacgg ctgggacgaa caccacgctg
    15481 tatctgaagc gcgaagattt gctgcacggc ggcgcgcata aaactaacca ggtgctcggt
    15541 caggctttac tggcgaagcg gatgggtaaa accgaaatca tcgccgaaac cggtgccggt
    15601 cagcatggcg tggcgtcggc ccttgccagc gccctgctcg gcctgaaatg ccgtatttat
    15661 atgggtgcca aagacgttga acgccagtcg cctaacgttt ttcgtatgcg cttaatgggt
    15721 gcggaagtga tcccggtaca tagcggttcc gcgacgctga aagatgcctg taacgaggcg
    15781 ctgcgcgact ggtccggcag ttatgaaacc gcgcactata tgctgggcac cgcagctggc
    15841 ccacatcctt atccgaccat tgtgtgtgag tttcagcgga tgattggcga agaaacgaaa
    15901 gcgcagattc tggaaagaga aggtcgcctg ccggatgccg ttatcgcctg tgttggcggc
    15961 ggttcgaatg ccatcggcat gtttgcagat ttcatcaacg aaaccaacgt cggcctgatt
    16021 ggtgtggagc cgggtggtca cggtatcgaa actggcgagc acggcgcacc gctaaaacat
    16081 ggtcgcgtgg gcatttattt cggtatgaaa gcgccgatga tgcaaaccga agacgggcag
    16141 attgaagaat cttactccat ctccgccgga ctggatttcc cgtctgtcgg cccacaacac
    16201 gcgtatctta acagcactgg acgcgctgat tacgtgtcta ttaccgatga tgaagccctt
    16261 gaagccttca aaacgctgtg cctgcacgaa gggattatcc cggcgctgga gtcttcccac
    16321 gccctggccc atgcgctgaa aatgatgcgc gaaaccccgg aaaaagagca gctactggtg
    16381 gttaaccttt ccggtcgcgg cgataaagac atcttcaccg ttcacgatat tttgaaagca
    16441 cgaggggaaa tctgatggaa cgctacgaat ctctgtttgc ccagttgaag gagcgcaaag
    16501 aaggcgcatt cgttcctttc gtcacgctca gggatccggg cattgagcag tcattgaaaa
    16561 ttatcgatac gctaattgaa gccggtgctg acgcgctgga gttaggtatc cccttctccg
    16621 acccactggc ggatggcccg accattcaaa acgccactct gcgcgccttt gcggcaggtg
    16681 tgactccggc acaatgtttt gaaatgctgg cactgattcg ccagaaacac ccgaccattc
    16741 ccattggcct gttgatgtat gccaatctgg tgtttaacaa aggcattgat gagttttatg
    16801 cccagtgcga gaaagtcggc gtcgattcgg tgctggttgc cgatgtgcca gttgaagagt
    16861 ccgcgccctt ccgccaggcc gcgttgcgcc ataatgtcgc acctatcttc atctgcccgc
    16921 caaatgccga tgacgacctg ctgcgccaga tagcctctta cggtcgtggt tacacctatt
    16981 tgctgtcacg agcgggcgtg accggcgcag aaaaccgcgc cgcgttaccc ctcaatcatc
    17041 tggttgcgaa gctgaaagag tacaacgctg cacctccatt gcaggggttt ggtatttccg
    17101 ccccggatca ggtaaaagca gcgattgatg caggagctgc gggcgcgatt tctggttcgg
    17161 ccattgttaa aatcatcgag caacatatta atgagccaga gaaaatgctg gcggcactga
    17221 aagtttttgt acaaccgatg aaagcggcga cgcgcagtta atcccacagc cgccagttcc
    17281 gttggcggca ttttaacttt ctttaatgaa gccggaaaaa tcctaaattc atttaatatt
    17341 tatcttttta ccgcttcgct tacccctgtc gaacgtcaac ttacgtcatt tttccgccca
    17401 acagtaatat aatcaaacaa attaatcccg caacataaca ccagtaaaat caataatttt
    17461 ctctaagtca cttattcctc aga
//
LOCUS       DAJTMC010000081        17341 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.081, whole genome shotgun sequence.
ACCESSION   DAJTMC010000081 DAJTMC010000000
VERSION     DAJTMC010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17341
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.081"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            189..2036
                     /gene="ldtD"
                     /locus_tag="OO130_002399"
     CDS             189..2036
                     /gene="ldtD"
                     /locus_tag="OO130_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR5365780.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            2217..2765
                     /locus_tag="OO130_002400"
     CDS             2217..2765
                     /locus_tag="OO130_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HCR5365781.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2792..3439
                     /gene="gloC"
                     /locus_tag="OO130_002401"
     CDS             2792..3439
                     /gene="gloC"
                     /locus_tag="OO130_002401"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HCR5365782.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3661..4851)
                     /gene="aspC"
                     /locus_tag="OO130_002402"
     CDS             complement(3661..4851)
                     /gene="aspC"
                     /locus_tag="OO130_002402"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HCR5365783.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARMAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(5036..6124)
                     /gene="ompF"
                     /locus_tag="OO130_002403"
     CDS             complement(5036..6124)
                     /gene="ompF"
                     /locus_tag="OO130_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HCR5365784.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYAHLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
     gene            complement(6726..8126)
                     /gene="asnS"
                     /locus_tag="OO130_002404"
     CDS             complement(6726..8126)
                     /gene="asnS"
                     /locus_tag="OO130_002404"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HCR5365785.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(8295..9497)
                     /gene="pncB"
                     /locus_tag="OO130_002405"
     CDS             complement(8295..9497)
                     /gene="pncB"
                     /locus_tag="OO130_002405"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HCR5365786.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYKKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            9763..12375
                     /gene="pepN"
                     /locus_tag="OO130_002406"
     CDS             9763..12375
                     /gene="pepN"
                     /locus_tag="OO130_002406"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HCR5365787.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPEHFTLKIVNEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKTKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPIVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVISLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQFHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            complement(12418..13169)
                     /gene="ssuB"
                     /locus_tag="OO130_002407"
                     /pseudo
     CDS             complement(12418..13169)
                     /gene="ssuB"
                     /locus_tag="OO130_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309043.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
     gene            complement(13166..13903)
                     /gene="ssuC"
                     /locus_tag="OO130_002408"
                     /pseudo
     CDS             complement(13166..13903)
                     /gene="ssuC"
                     /locus_tag="OO130_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001714028.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
     gene            complement(13968..15113)
                     /gene="ssuD"
                     /locus_tag="OO130_002409"
                     /pseudo
     CDS             complement(13968..15113)
                     /gene="ssuD"
                     /locus_tag="OO130_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
     gene            complement(15110..16069)
                     /gene="ssuA"
                     /locus_tag="OO130_002410"
     CDS             complement(15110..16069)
                     /gene="ssuA"
                     /locus_tag="OO130_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HCR5365788.1"
                     /translation="MRNIIKLALVGLLSVSTIAVAAESSPEALCIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            complement(16062..16637)
                     /gene="ssuE"
                     /locus_tag="OO130_002411"
     CDS             complement(16062..16637)
                     /gene="ssuE"
                     /locus_tag="OO130_002411"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HCR5365789.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFVP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSALKAQEILHGVFADDSQVIDYHHRPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLLSLRGNAHA"
     gene            16993..>17341
                     /locus_tag="OO130_002412"
     CDS             16993..>17341
                     /locus_tag="OO130_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5365790.1"
                     /translation="MKKSVLTAFITVVCATSSVMAADDNAITDGKVTFNGKVIAPACT
                     LVAATKDSVVTLPNVSATKLQTNGAVSGVKTDVPIALEGCDVTVTKNATFTFSGTADG
                     VQPTAFANQATTDA"
BASE COUNT         4371 a         4434 c         4398 g         4138 t
ORIGIN      
        1 acggcgcgct tttctttaaa cttctttaca ttaggttatg taaaaacgta tcggcgttta
       61 tatactgaag ataagcctga tgagtaacag gcttgctcgt catactttcg tgagtattgg
      121 cgttgtacag gcaagtcgta aaataacagc ctggctattc agagtatgat aaaaacaggg
      181 ggcaagggat gttgcttaat atgatgtgtg gtcgtcggct gtcggcaatc agtttgtgcc
      241 tggccgtaac attcgctcca ctgttcaatg cgcaggccga tgagcctgaa gtaatccctg
      301 gcgacagccc ggtggctgtc agtgaacagg gcgaggcact gccgcaggcg caagccacgg
      361 caataatggc ggggatccag ccattgcctg aaggtgcggc agaaaaagcc cgcacgcaaa
      421 tcgaatctca attacccgca ggttacaagc cggtttatct taaccagctt caactgttgt
      481 atgccgcacg cgatatgcaa cccatgtggg aaaaccgtga tgctgttaaa gccttccagc
      541 aacagctggc agaggtggcg attgccggtt tccagccgca gtttaataaa tgggtagagt
      601 tactgaccga tcctggtgtt aacgggatgg cacgcgacgt agtgctctct gatgcgatga
      661 tgggctatct ccatttcatt gcaaatattc cggtcaaagg cactcgctgg ctatatagca
      721 gtaaacctta tgcacttgca acgccgccgc tctcggtgat taaccaatgg cagctggcgc
      781 tggataaagg tcaattgcct acgtttgttg caggactggc accgcagcat ccgcaatatg
      841 cggcgatgca tgaatcgtta ctggccttac tcagtgacac caaaccgtgg ccccaactga
      901 ccggcaaagc aacgttgcgc ccagggcagt ggagtaacga cgtaccggcg ttgcgcgaaa
      961 tattgcaacg cacaggcatg ttggacgggg ggccgaaaat tactctacct ggcgatgaca
     1021 cgccaactga cgcggtagtc agcccatccg ctgttactgt tgaaacagca gaaactaagc
     1081 cgatggacaa gcaaacgacg tctcgtagta aacctgcgcc tgccgttcgc gccgcctacg
     1141 ataatgaact ggtggaagcc gttaaacgtt ttcaggcatg gcaaggattg ggggcagatg
     1201 gtgctattgg cccggcaaca cgtgactggt taaacgtaac gcccgcccag cgtgctggcg
     1261 tgttggctct caacatccag cgattgcgct tgctgccaac agagctttct accgggatca
     1321 tggttaacat tccggcctat tcgctggtct actatcagaa cggcaatcag gtgctggatt
     1381 cgcgagtcat tgtcggtcgc cccgatcgca aaacgccgat gatgagcagt gcccttaaca
     1441 acgtagtggt aaacccgccg tggaacgtac ctccaactct ggcacgcaaa gatattctgc
     1501 caaaagtgcg caacgatccg ggatatctcg aaagccatgg ctatacggtg atgcgtggct
     1561 ggaacagcag agaagcgatt gacccatggc aggttgactg gtctacaatc acggcctcga
     1621 atttaccgtt ccgcttccag caggctccag gcccacggaa ctcgctgggg cgctataaat
     1681 tcaatatgcc gagttcagag gccatttatt tgcatgacac gccgaaccac aatctgttca
     1741 agcgtgatac acgcgcattg agctcaggct gtgtacgagt gaataaagct tccgatctgg
     1801 cgaatatgct gttgcaggat gcaggctgga atgacaaacg tatttctgat gcgctgaagc
     1861 agggtgatac acgttacgtc aatattcggc agtcgattcc ggtgaatctc tactacctga
     1921 cggcctttgt tggtgcagat ggtcgtaccc agtatcgtac agatatttac aattatgatc
     1981 tgcctgcgcg atccagctcg caaatcgtat cgaaagcgga acaattaatc aggtaaatga
     2041 agtagttcgg ggaaatcagt tgtcgtaata attagcatga ttgggggcga ttctctgcag
     2101 cccccgtcac tgctggggtt gagtcatctt gacgtctgct ttacgggcgg ttaaggtgcc
     2161 tcttgtgcgc cagaagtgca tataaacgat aacattgacc tgtagacttg attatcatgg
     2221 acaaattcga cgctaatcgc cgcaaattgc tggcgcttgg tggcgttgca ctcggtgccg
     2281 ccatcctgcc gacccctgcg tttgcaacac tctctacccc acgcccgcgc attttgacac
     2341 tcaataatct tcataccgga gagtcaatca aagcggagtt tttcgatggc agaggctata
     2401 ttcaggaaga attggcaaaa cttaaccatt ttttccgcga ttaccgcgcg aacaaaataa
     2461 agtccatcga cccaggatta ttcgaccagt tgtatcgcct gcaagggttg ttaggcacgc
     2521 gcaaaccggt gcaactcatt tccggttatc gttccattga taccaacaat gaactacgcg
     2581 cccgcagccg tggagtagcg aagaaaagct atcacactaa aggccaggcg atggatttcc
     2641 atattgaagg tatcgcgtta agcaatattc gcaaagccgc gttatctatg cgcgcaggtg
     2701 gtgtaggata ttacccacgt agtaactttg tgcatattga taccgggcca gcacggcact
     2761 ggtagcaatc gcttaacgaa acaggggcag tatgaactat cgtattattc cggtcaccgc
     2821 attctcccag aactgttcat taatctggtg tgaacaaacc cgtctggccg cactggtcga
     2881 tcctggcggc gatgcggaaa aaatcaaaca ggaagttgat gacagcggcc tgacactgat
     2941 gcagatcctg ctgacgcatg gtcatctgga ccacgttggc gcagcggcgg aactggcgca
     3001 acattacggc gtgccggttt ttggcccgga aaaagaagat gagttctggc tgcaaggctt
     3061 gcctgcgcaa agtcgtatgt ttggtctgga agagtgccag ccgctgacgc cagatcgttg
     3121 gctgaacgaa ggcgatacca tcagcatagg aaatgtgact ttacaggtgt tacattgccc
     3181 tgggcatacg ccgggtcatg tcgtgttttt tgatgatcgg gcaaagctgc tgatttctgg
     3241 cgatgttatt ttcaaaggcg gagtagggcg cagtgacttc ccgcgtggcg atcataatca
     3301 actgatttct tcaatcaaag ataaattgct gccactgggg gatgacgtga catttattcc
     3361 gggtcacgga ccattatcca cacttggtta tgaacgcctg cataatccct tcctgcaaga
     3421 cgaaatgccc gtctggtaag gcacataaaa aagcccgctt ttaatgcggg cctggatttc
     3481 tggcaaagtg cgctttgttt atgccggatg cggcacgagc gccttatccg gcctacaaaa
     3541 tcgtgcaaat tcaaaatatt gcaggggacg cgtaggcctg ataagcgtag cgcatcaggc
     3601 aatgttgcgt ttgtcatcag tctcagcccg cttttcagcg ggcttcattg tttttaatgc
     3661 ttacagcact gccacaatcg cttcgcacag cggagccatg ttatctggtg tcatcccggc
     3721 cacatttacg cgaccagaag caaccgcata tacgccaaac tcttcgcgca gacgcagcac
     3781 ttgttctttt gtcaggccac tgaaggagaa catgccgttc tgtttgatga taaagctgaa
     3841 gtcgcggttt gcgccttttt cctgcagcgt attgacgaac aactgacgca tacgctgaat
     3901 acgctggcgc atatcagtca gctcttgttc ccaaatcgca cgtaacgcat cgttgctcag
     3961 gatggtggca acaacagaag cgccgtgtgc tggtgggtta gagtagttag cgcgaatcgc
     4021 cgctttcatt tggctgaatg cgcgatcaac ggtttcactg tcggcagcaa ccagagtaca
     4081 agcgccaaca cgctcgttgt acaggccaaa gtttttagag taggaactgg caacaatcag
     4141 ctctttatgc atagccgcga aagcgcgcag tccttcagca tcttcttcca gaccacgggc
     4201 aaaaccctgg taagcgaagt caaacagcgg taaccagcct ttctcaacgg agagttgtgc
     4261 cagtgtctgc cattgttcca gcgtagggtc gataccggtt gggttatggc agcagccatg
     4321 gaacagcact acgtcgccag cctgagcttc attcaggctg ttaatcagtg catcgaagtc
     4381 aagagtgtga ttttccgcat cataataagc gtattcacga acttccagac ctgcagagtt
     4441 aaagacgctc ttatggttcg gccagcttgg gttgctcacc cacactcgct taacgctggt
     4501 attttttgcc aggaaatcgg cagctacgcg tagtgcgcca gtgccccccg gagtctgtgc
     4561 catgcgagca cgtttgtcat tgatcagggc gctaccttta ccaaacagca gttcctgagt
     4621 gcagcgacca aattcaggga tgccgtcaat gccgaggtaa tttttggtgg tttcattttc
     4681 gagcagatac tgttcagcct ttttcacgct ggtcagtacc ggggttttgc ccgtctcatc
     4741 tttatagaca ccaatcccga ggttaatttt gccgggacgt tcatcggcac gaaacagatc
     4801 ggccaggccc agaatcgggt cggcaggagc ggcggtaatg ttctcaaaca tgacgaggtt
     4861 ccattatggt tacagaaggg aagtccgcta tcagggtaac gggagattta caaaattcca
     4921 actattactg atgaaaacgc aggctgtttt tgcaagacgt gagattgctc tggaaggtat
     4981 aaaaaaaaca ggaccaaagt cctgtttttt cggcatttaa caaagaggtg tgctattaga
     5041 actggtaaac gatacccaca gcaacggtgt cgtctgaacc tacgcccagt ttgttatcag
     5101 aatcgatctg gttgatgatg tagtcaacat aggtggacat gtttttgttg aagtagtagg
     5161 ttgcgcccac ttcaaagtag ttcaccagat caacatcacc gataccttct acgtctttcg
     5221 ctttagattt ggtgtaagcg atggacggac gcagaccgaa atcgaactgg tattgcgcaa
     5281 ctaacagaac gtcttgcgtt ttgttggcga agccgctggt gtttgtaaat ttattagtga
     5341 tcggcgtagc gttacgggtt tcaccgtagt tcgctgccag gtagatgttg ttcgcgtcgt
     5401 acttcagacc agtagcccac tgttcagctt ttttaccgtt gccaagaggt tgagcttctt
     5461 gcaggttggt acggtcagct gcaccataag caccaacgat accaaagcct tcgtattcgt
     5521 agctgataga accgccaaca ccgtcgccgt tagaacggcg tgcagtgtca cgctcgtttt
     5581 tacccaggta ctgaacagcg aagttcaggc catcaaccag accaaagaag ttggagttac
     5641 gataggtagc aacgccgcca acacgaccaa cgaagaagtc atcgctgtat gcagtatcac
     5701 caccaaattc tggcagcata tcggtgtaac ccagtgcatc ataaaccaca ccgtagttac
     5761 ggccgtaatc gaaagaacca acgtcagcgt atttaagacc cgcgaatgcc agacgcgttt
     5821 tgttaccagt ttgagcgtca gcgccttcag agttgttacc ttggaagtta tattcccact
     5881 gaccataacc ggtcagatcg gaattgattt gagtttcccc tttaaaacca agatgggcat
     5941 aggtcatgtc gccattgcca ccgtaactgt tttcaccgtt acccttggaa aaataatgca
     6001 gaccaacagc tttaccgtac agatctactt tgttgccatc tttgttatag atttctgcag
     6061 cgtttgcagt acctgctact aacagagcag ggacgatcac tgccagaata ttgcgcttca
     6121 tcattattta ttaccctcat ggtttttttt atgacacctg ccactgccgt caataagttc
     6181 tgtcaataaa aatttacgga actattgatg agagtttggt gtctttatgt gtctgcaggc
     6241 atctttccat tcaaactaac gtttcgctac cgtgaaagtg ctgcaaagat aaagatttag
     6301 tttcaaaaag aaaaaatatg taaccaaaag taaaaattaa ggaactttgt gaacaccgtc
     6361 atatttccat agaaacgtga tgatatttac agcaattttt atctatttat atgatttact
     6421 tatatttaaa ttaactaagc ggaaattttg tttctgatgg aaactttatc gacctggcac
     6481 aaaatcctct ttcagacatc cagaatgcca aaaaatagta tgaaaattgt gctatcagct
     6541 aagaaaaaac taatccgcaa taagaatacg ggttattttt tgtgcgtaaa tgttgcaatg
     6601 taatattccg taacaggatg atcgttatgg cggggttgca aatagattgc ttgacttaac
     6661 aaacagataa cttgacagaa aggataaaaa agagccagca ggcgctggct ctttgaggaa
     6721 gaaaatcaga agctggcgtt gcgcggagta cgtgggaacg gaatcacatc acgcacgttt
     6781 tgcacgccag ttacgtaagc aatcaggcgt tcaaaaccaa gaccgaaacc tgaatgcgga
     6841 acagtaccgt agcgacgcag atcgcgatac caccagtaat cttctttatt caggcccatt
     6901 tccagcatac gctcgtccag aacgtccaga cgttcttcac gctgggagcc tccaatgatc
     6961 tcaccgatgc ccggagccag aacgtccata gccgcaacgg ttttaccgtc ttcgttgagg
     7021 cgcatataga acgctttaat atctttcgga tagtttttaa ccactaccgg tgctttaaag
     7081 tgttcttccg ccagataacg ctcatgctca gaagagagat cgacgcccca gtaaaccggg
     7141 ttttcaaact tcctgccgca gttttcgaga atggtcactg cgtcggtgta atccacctgc
     7201 gcgaaatcgg cctcaatgaa gcgttccaga cgtgaaacgg catctttatc tacgcgttca
     7261 gcgaagaatt tcatgtcgtc tgcgcgttct tcgagaaccg ctttgaagac atatttcagc
     7321 atggcttcag ccagacccgc gatatcgttc aggttagcaa acgccacttc cggctccagc
     7381 atccagaatt ccgccaggtg acggctggtg ttggagtttt cagcacggaa agtcgggccg
     7441 aaggtataaa ttttggataa tgcgcaagcg taggtttcgc cgttcaactg gccagatacg
     7501 gtcaggaaag actctttacc aaagaagtct ttgtcgaaat ccactttgcc ctgatcgtta
     7561 cgcggcaggt tttccagatc cagcgtagaa acgcggaaca tttcgcctgc accttcggta
     7621 tcagatgcgg taatcagtgg cgttgaaacc cagaagaatc cctgctcgtt aaagaagcga
     7681 tgcagcgcct gcgccagcgt atggcgaacg cgcgcgacgg caccaatcag gtttgtgcgc
     7741 ggacgcaggt gagcgacttc acgcagatac tcaatgctgt ggcgttttgc cgccatcggg
     7801 taagtgtctg gatcttcaac ccaaccagca acttcaactt tgctggcctg aatttcaaat
     7861 tgttgcccct ggcccggcga cgccacgact ttacccgtca caatgaccga gcagccggtg
     7921 gtcagacgca ggacgtcttc attgtaattg ggcagagaat tattgatgac agcctgtaca
     7981 ggatcaaagc aggaaccgtc ataaacggcg aggaaggaga tgccagcttt tgaatctcgg
     8041 cgggtacgta cccatccgcg tacggtgact tcgctgtcaa cggctacgcg gccctggagt
     8101 acgtcggcta caggcacaac gctcataata ttctctctgt taatagtcgg aaaaaataaa
     8161 cacttgtcca cccgtaaatg gggggatacc tatgttacct ggcatctgca atcagacaag
     8221 cagaattcgc aaatgcagcg aaagatttcg gaattaaagg taaaaaaagg gagctgatta
     8281 gctccctgat gatattaact ggctttttta atatgcggaa ggtcgaacgc tttgcgcagc
     8341 gcccgaacaa acgctttatc atggcagata gttttgccag ggctgtcaga aagtttcgcc
     8401 accggtttac cgttacactc taccaactta atcacaatat tcaggggttt tacctggggg
     8461 atatcgcagg tcaggcgagt cccaatacca aaacttaatt gcacgcggga agagaagtgg
     8521 cggtatagct caaccgcttt gcgtaaatcc agattgtcag agaaaaccag cgttttactc
     8581 tgtggatcaa ttcccagctt tttataatgt gcaatggctt tttcacccca ttcaaccggg
     8641 tcgccagagt catgacgcag gccctgatac cgactggcga actcgacacc gaaatcacgc
     8701 aggaaagcat ccatagtgat gcagtcggtt aatgcaatgc caagttggtc gggatattct
     8761 tccagccagg cagcaagtgc agctcgctgg ctgttggcta ggtccgggct gatttgctga
     8821 tgtgcctgga accattcgtg cgcctgtgtt cccatcggcg tgagggaaag ccgacgcgcc
     8881 agatcgtagt tgctggtgcc cacgaaccag gattcctgtt gcagacgctt aacgatggtt
     8941 tcttgtactt cgcgagaaaa acggcgacgg gtgccaaaat ccatcagatg gaagcgcgac
     9001 atatcaagac cggcggttaa cgccgagaag tcgactaatt tgctttccag cgtgtcgagg
     9061 gcttgcgcaa cgtcggcctg cggtgagcga tagcgatgta ccatttcact gataaccgcc
     9121 agcaaaggaa cttcccagag gatgacttca cgccacggac cgcttaagcg aatatccagc
     9181 ttgccattat cgttggacac ggtgacttgt tccgggttaa agcggaattc gcgtaaccag
     9241 ttaagatagt cggccttaaa gaaaggcagg gcagagagcc actgatattc atcatcctgc
     9301 aggcgcaggt gctgcatcgc ctgaatctgt tcacgaatag catcggcata aatacccagc
     9361 agatcgtcac ctcggcaacg aaactccgcc gcgacatgca catcgtaata gtgatgaaac
     9421 acggcttgct gcatatgcaa cttataagca tctgtatcca gcaacgagtg cagaacagga
     9481 gaagcgaatt gtgtcatagg tgcgcagtag catcctcgta caggagcgtt tagtacaata
     9541 aacatcttca ggaactgctg gagtatacct tgtttcgcaa tttattgaac cccgatcaca
     9601 ccatatgcca cctttctggt cgatggcatt acgcggtgta tgttataaaa atgtagcaat
     9661 aaaggcgttt gtacctgaaa agatgaagat tctgcatagc gcgatttacg caacaggaat
     9721 agactgaaca ccagactcta taaaagatgc taaaggttat ttatgactca acagccacaa
     9781 gccaaatacc gtcacgatta tcgtgcgccg gattaccaga ttactgatat tgacttgacc
     9841 tttgacctcg acgcgcaaaa gacggtcgtt accgcggtca gccaggctgt ccgtcatggt
     9901 gcatcagatg ctccgcttcg tctcaacggc gaagacctca aactggtttc tgttcatatt
     9961 aatgatgagc cgtggaccgc ctggaaagaa gaagagggcg cactggtcat cagtaatttg
    10021 ccggagcatt ttacgcttaa gatcgttaat gaaataagcc cggcggcgaa taccgcgctg
    10081 gaagggcttt atcagtcagg cgatgcgctt tgcacccagt gtgaagccga aggtttccgc
    10141 catattacgt attatctcga ccgcccggac gtgctggcgc gttttaccac caaaattatt
    10201 gccgataaaa ccaaatatcc cttcctgctt tccaacggta accgcgttgc gcaaggtgaa
    10261 ctggaaaacg gacgccattg ggtacagtgg caggacccgt tcccgaaacc gtgctacctg
    10321 tttgcgctgg tggcaggcga ctttgatgta ctgcgcgaca cctttaccac gcgttctggt
    10381 cgcgaagtgg cgctggagct gtacgtcgat cgcggcaacc ttgatcgcgc gccgtgggcg
    10441 atgacctcgc tgaaaaactc aatgaaatgg gatgaagaac gcttcggcct ggagtatgac
    10501 ctcgacatct atatgatcgt cgcggtggat ttcttcaata tgggcgcaat ggagaataag
    10561 ggtttgaata tctttaactc caaatatgtg ctggcccgca ccgacaccgc cactgacaaa
    10621 gattacctcg atattgaacg cgttatcggc catgaatatt tccataactg gaccggtaac
    10681 cgagtcacct gccgcgactg gttccagctc agcctgaaag aaggtttaac cgtcttccgc
    10741 gatcaggaat tcagctctga ccttggttcc cgtgcagtta accgcatcaa taatgtgcgc
    10801 accatgcgcg gattgcagtt tgcggaagac gccagcccga tggcgcaccc gatccgcccg
    10861 gacatggtca ttgagatgaa caacttctat accctgaccg tttacgagaa gggcgcggaa
    10921 gtgatccgta tgatccacac cctgctgggc gaagaaaact tccagaaagg gatgcaactc
    10981 tatttcgagc gccatgatgg cagcgcggca acctgcgacg actttgtgca ggcgatggaa
    11041 gatgcgtcga atgtcgatct ctcccatttc cgccgttggt acagccagtc cggcacgccg
    11101 attgtgaccg tcaaagacga ctacaatccg gaaaccgagc agtacaccct gaccatcagc
    11161 cagcgcacgc cagccacgcc ggatcaggca gaaaaacagc cgctgcatat tccgtttgcc
    11221 atcgaattgt atgacaacga aggcaaagtg atctcgttgc agaaaggtgg tcatccggtg
    11281 aattccgtgc tgaacgtcac ccaggcggaa cagacctttg tttttgataa tgtctacttc
    11341 cagccggtgc ctgcgctgct gtgcgaattc tctgcgccag tgaaactgga atataagtgg
    11401 agcgatcagc aactgacctt cctgatgcgt catgcgcgta atgatttctc ccgctgggat
    11461 gcggcacaaa gcctgctggc aacctacatc aagctgaacg tcgcccgaca tcagcaaggg
    11521 cagccgctgt ctctgccggt acatgtggct gacgctttcc gcgcagtgct gctcgatgag
    11581 aagattgatc cggcgctggc ggcagaaatc ctgacgctgc cttctgtcaa tgaaatggct
    11641 gaactgttcg atatcatcga cccgattgct attgccgaag tacgcgaagc actcactcgt
    11701 actctggcga ctgaactggc ggatgagctg ctggctattt acaacgcgaa ttaccagagc
    11761 gagtaccgtg ttgagcatga agatattgcg aaacgcactc tgcgtaatgc ctgcctgcgc
    11821 ttccttgctt ttggtgaaac gcatctggct gacgtgctgg tgagcaagca gttccacgaa
    11881 gcgaacaata tgaccgatgc gctggcggcg ctttctgcgg cggttgccgc acagctgcca
    11941 tgccgtgatg cgctgatgca ggaatacgac gataagtggc atcaggacgg tctggtgatg
    12001 gataaatggt ttatcctgca agccaccagc ccggcggcga atgtgctgga gacggtgcgc
    12061 ggtctgttgc agcatcgctc atttaccatg agcaacccga accgtattcg ttcgttgatt
    12121 ggcgcgtttg cgggcagcaa tccggcagcg ttccatgccg aagatgggag tggttaccag
    12181 ttcctggtgg aaatgcttac cgacctcaac agccgtaacc cgcaggtggc ttcacgtctg
    12241 attgaaccgc tgattcgcct gaaacgttat gatgccaaac gtcaggagaa aatgcgcgcg
    12301 gcgctggaac agttgaaagg gctggaaaat ctctctggcg atctgtacga gaagatcacc
    12361 aaagcactgg cttgataaat aaccgaatgg cggcaatagc gccgccattc ggggaattta
    12421 cccctgtttt ctcaggcgaa tttcagattc accacgctgc atcacccttt gcaacacttc
    12481 cgattccagc tccgctaatc gcaccgaccc taatcgacgc ggagggggga tatccactgt
    12541 caaatccaga ccaatttttc cctcttcaat taataacacc cggtcagcca ttgctaccgc
    12601 ttcgctcaca tcatgcgtca ccaacagtac ggtaaagccg tgctcctgcc aaagtgacac
    12661 aatcaaatcc tgcatctcga gtcgcgttaa ggcgtccagt gccccgagcg gttcatcaag
    12721 caacaataaa cccggtcgat gaatcaacgc tcttgccagc accactcgct gtttctgccc
    12781 gcctgaaagt gcggcaggcc attccccggc gcgattctcc agtcctaccg cagccaacgc
    12841 ccgacgcgcg gcatcgcgcc actggccttt aaggcctaac ccaacgttat caatcaccga
    12901 tttccacggt agcagacgcg catcctgaaa catcattcgc gtgtcttcct gaatttcagc
    12961 cagcggtgtg gtgcctgcca acacatcgcc tgcggttggc gtttccagcc cggccagcag
    13021 gcgcagcagg gtacttttgc caccaccgcc acaaactgac ctgccggaat atgtaaatcc
    13081 agttggttca ggacgatatt ttccgcgtaa tgtttgctta ctgcattgag caacaatggt
    13141 gtgccctggt tcagacgagc agtattcata ccgtggcctc cttcaaatga taagccgggt
    13201 tccagcgcaa ccagaggcgc tctaacagct gcgcgctgac gtcagcaagt ttgccgagca
    13261 gggcgtaaag aataatagcg actaccacca cgtccgtttg caaaaactct cgcgcattca
    13321 tcgccagata accaatgccg gaattggcag aaatggtttc ggcaacaatc agcgtcagcc
    13381 acatcaggcc caacgcaaaa cgcacgccga ccataattga gggcagggca ccaggcagga
    13441 tcacatggat aaacagcggt atgccggata atccatagct acgcgccatc tccaccagcc
    13501 cgcgatcgat attacggatg ccatgccagg tgttgatata aatgggaaac agcgtaccga
    13561 gcgccaccag aaagattttt gcggattcat caatgccaaa ccacaaaatc accagcggga
    13621 tcagcgccag atgcggcacg ttgcgcaaca tctgaattga ggtatccagc agccgttctc
    13681 cccagcgaga tagcccgcta atcagtccca aaatcaaccc cagcgatccg ccaattgaaa
    13741 agccaatcag cgcccgctag gaactgatcg ccagatgctg ccacagttcg ccgctggcgg
    13801 agagcgtcca gaacgccgtc accacccctt ccggtgaggg caaaatacgc gtcgacagcc
    13861 agccaactga ggaggccagt tgccacaccg ccacgatgcc catcggtaaa aactaggggg
    13921 caacgcgcaa taaccacttc ttcactggcg ttgccattat ttcttcctta gctttgcgcg
    13981 actttacggg ggataaaatc attcgccacc gcttcgcctt gcggattcag cggctgcggc
    14041 tggggaattt ccggaatggc gacatccaga tgtgggaaca gcaactcgcc gacccgatac
    14101 gcttcttcca gatgcggata gcccgaaagc acaaagctgt cgatgccaag cgcggcgtat
    14161 tcgttgattc gcgcagcgac cgtaggacca tcgcccacca gcgccgttcc ggcaccgccg
    14221 cgcactaagc caacgcccgc ccataaattg gggctgatct ccagattgtc gcgcttgccg
    14281 ttatgtaacg ccgccattcg ctgttgtcct acggaatccg tccgggcgaa tgcggcctgt
    14341 gctttggcga tagtttcatc atcaagatgc gagattaacc gctcggcggc ctgccacgct
    14401 tcgtcgttag tttcacgaac aatcacatgc agacgaatac cgaaacgaat tttgcgtcca
    14461 tgcgcggcag ctttcgcccg cacttgttcg atcttctctt ttactagttc tggcggttcg
    14521 ccccaggtga ggtagagatc aacttgttct gccgccagct cctgggcgac gtctgacgat
    14581 ccgccaaagt aaagcggagg ataaggctgt tgaatcggcg ggaagagcag ttttgctccg
    14641 cgcacatgaa tatgtttacc gttgaaattg acggtttccc ccagcaataa gcgccgccag
    14701 acctaggtaa attccgccga ggcttcgtag cgctcgctat gatcaaggaa cacgccgtcg
    14761 cctgccagct cttgtggatc gctgcctgtg accagattaa acaacgcacg accatttgag
    14821 agacggtcaa gcgtggcggc ctggcgagcg gcaacggtag gtgaggttac gctggggcgc
    14881 agggcgacaa gaaacttcag ccgctgcgtc accgggatca tcgacgccgc caccagccac
    14941 gcatcttcac acgatcgccc cgttgggatc agcacaccgg tatagcccag acgatccgcc
    15001 gcttgcgcaa tttgttgcag ataaccgtga tcaaccgggc gtgaaccttc ttccgttccc
    15061 agatagtgcc cgtcaccgtg ggtcggtaaa aaccagaaca tattcagact cataattgtt
    15121 ttccttccag ttgagtgggc tgccagatgc gctggcgaat atcgactttt ttcggcacca
    15181 gacgattttc ataaaacaga tctgccgttt gctgctgtaa ggcggcaacc tcggcgttaa
    15241 ccggtttgat ggtggtagga gggcgatgat ctaagtaaga ggcaatcacc ggtgccggta
    15301 agcccatcgt ttttgccagt aaagcgatgc tttgctcgcg ctggctgcgg gttaacgcat
    15361 cggcctcact aaaggttgcc agtacgccct gaataaaagc gccgtttttt tctgcatagg
    15421 gacgagctgc cagataaaac gatccagttt gattgagatc ggtgccgtct ttcagcaccc
    15481 gcacgccgcc ctgtaataat gcagcagagt agtagggatc ccagatagcc caggcgtcaa
    15541 cgttaccttg ctggaacgcg gcgcgggcat cagcgggcgt caggtaagtg ggttggatat
    15601 cggtaaactt aagtccggcc tgacgcagtg cacgcagtaa aaggttgtgt gaactggaac
    15661 ctttctgaaa ggcaactttg tgacctttaa gatcggctac ggttttgatc gggctgtttt
    15721 ctgccaccag aatcacttcg gctttgggct tcggtggctc gacgcccaca tataccaaat
    15781 cagccccggc agcctgggca aagattggcg gaatatcccc ggtactgccg agatcaatac
    15841 tgccaacgtt taaggcttcc aacatttgcg gacccgcggg gaactccacc caggagattt
    15901 ttgattgcgg atagcgtttt tccagtaact ggtggctttt tgccagtacc ataccaatac
    15961 tgcctttctg atagcctata cataacgctt caggcgagga ttctgcagca accgcaatcg
    16021 tagagacgct aagcaatccc accagcgcca gtttaatgat gttacgcatg ggcattacct
    16081 cgcagagaca gaaggtcagg aacctgaaca tcgcggcggt gcaatgcctg ccagaaagtt
    16141 tctagcgcgg tatcaagacg ggtttgcaga tttggcgtga actggggtct gtgatggtaa
    16201 tcaattactt gtgagtcatc ggcaaacacg ccgtgcagga tctcctgagc tttcagtgcg
    16261 cttaaaactg gtttaagggc ataatcgacc gccagcagat gggccacggt accgcccgtc
    16321 gccagcggta gcaccacttt gccttgcaaa gcgcgctctg gcagcaggtc gagcagggtt
    16381 ttcagcgcgc cggaataggc ggctttatac acaggtgtag cgactatcag tccatcagcc
    16441 tgttgcagct gttcggtgaa ggtcttgagt gccggactat cgaaacgagc ataaagcaga
    16501 tcttccggga cgaagttttg cagattccag tgataaacct ctacatccag gccatttagt
    16561 ttttcccgcg catattccag caaggagctg gagcgagaag gaaagcgagg actacccgcc
    16621 agggtgatga cacgcatact ctctccttat aaccaattgt tctttttttg ttaacattga
    16681 taacaattcg gtcagtctgt cggagagaca agaaaatgcc aaatataaat tttgtgtatc
    16741 tttttctgaa aataaactaa agaaagggct ttatatatca cgcatattta tttgttggat
    16801 agtcattaga tgttgaatca atgaattact cgttcagtta atatttaaat attcatgaaa
    16861 tctataaatt aaagatttgt cacttattgg atttagtatg cttgatttaa atgacggtca
    16921 attattgtta ttattaagtc taatgtcatt taaaggcatt atcatattta cgaagagatc
    16981 aggatgatga cgatgaaaaa aagtgtattg acggcgttta taactgtggt atgtgcaacg
    17041 tccagcgtta tggcagctga tgataatgct atcacggatg gcaaagtaac atttaatggt
    17101 aaagttattg ctccagcttg taccctggta gctgcgacga aagattcagt agtcacattg
    17161 ccaaatgtca gcgccaccaa gttgcaaact aatggagcag tctctggcgt taaaactgat
    17221 gtaccaattg ccttagaagg ctgtgatgtt actgtaacta aaaatgcgac gtttaccttt
    17281 agtggtacgg ctgacggcgt gcaacctaca gcatttgcca accaggctac caccgatgca
    17341 g
//
LOCUS       DAJTMC010000082        17108 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.082, whole genome shotgun sequence.
ACCESSION   DAJTMC010000082 DAJTMC010000000
VERSION     DAJTMC010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17108
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.082"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(<1..307)
                     /gene="hlyE"
                     /locus_tag="OO130_002413"
     CDS             complement(<1..307)
                     /gene="hlyE"
                     /locus_tag="OO130_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
                     /protein_id="HCR5365791.1"
                     /translation="MTEIVADKMVEVVKNAIETADGALDLYNKYLDQVIPWQTFDETI
                     KELSRFKQEYSQAASVLVGDIKTLLMDSQDKYFEATQTVYEWCGVATQLLAAYILLFD
                     "
     gene            680..1099
                     /locus_tag="OO130_002414"
     CDS             680..1099
                     /locus_tag="OO130_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HCR5365792.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            1099..2366
                     /gene="umuC"
                     /locus_tag="OO130_002415"
                     /pseudo
     CDS             1099..2366
                     /gene="umuC"
                     /locus_tag="OO130_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
     gene            complement(2412..2942)
                     /gene="dsbB"
                     /locus_tag="OO130_002416"
     CDS             complement(2412..2942)
                     /gene="dsbB"
                     /locus_tag="OO130_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HCR5365793.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGLEMPQWLLGIFIAYLIVAVLV
                     MISQPFKAKKRDLFGR"
     gene            complement(3088..4629)
                     /gene="nhaB"
                     /locus_tag="OO130_002417"
     CDS             complement(3088..4629)
                     /gene="nhaB"
                     /locus_tag="OO130_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HCR5365794.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            4851..5570
                     /gene="fadR"
                     /locus_tag="OO130_002418"
     CDS             4851..5570
                     /gene="fadR"
                     /locus_tag="OO130_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HCR5365795.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(5622..7154)
                     /locus_tag="OO130_002419"
     CDS             complement(5622..7154)
                     /locus_tag="OO130_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HCR5365796.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSVELLERCPPRMG
                     NL"
     gene            7484..8782
                     /gene="dadA"
                     /locus_tag="OO130_002420"
     CDS             7484..8782
                     /gene="dadA"
                     /locus_tag="OO130_002420"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HCR5365797.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            8792..9851
                     /gene="dadX"
                     /locus_tag="OO130_002421"
                     /pseudo
     CDS             8792..9851
                     /gene="dadX"
                     /locus_tag="OO130_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
     gene            complement(10238..11974)
                     /locus_tag="OO130_002422"
     CDS             complement(10238..11974)
                     /locus_tag="OO130_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HCR5365798.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGTAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(12069..12983)
                     /gene="ldcA"
                     /locus_tag="OO130_002423"
     CDS             complement(12069..12983)
                     /gene="ldcA"
                     /locus_tag="OO130_002423"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HCR5365799.1"
                     /translation="MSLFHLIAPSGYSIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNKTFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVSAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGTQLTISGHPVLKM"
     gene            13083..13694
                     /gene="emtA"
                     /locus_tag="OO130_002424"
     CDS             13083..13694
                     /gene="emtA"
                     /locus_tag="OO130_002424"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HCR5365800.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(13696..14430)
                     /locus_tag="OO130_002425"
     CDS             complement(13696..14430)
                     /locus_tag="OO130_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HCR5365801.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            14631..14885
                     /locus_tag="OO130_002426"
     CDS             14631..14885
                     /locus_tag="OO130_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCR5365802.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFQTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGLFRLLRRE"
     gene            15064..15504
                     /locus_tag="OO130_002427"
     CDS             15064..15504
                     /locus_tag="OO130_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5365803.1"
                     /translation="MIQKINILDSSQSGVKNGADSEAVLAALIEHLELINPSGRLSQN
                     TRSAMLQLREEEWSEFFFWLLNSLECLDYVIINLTPESKKTLMSEHRNNIQVAIDALY
                     RQRRCKSPGDDSETLTRRNDAIFGNHVWQTFAQYFPPGLEKPAV"
     gene            complement(15583..17108)
                     /locus_tag="OO130_002428"
                     /pseudo
     CDS             complement(15583..17108)
                     /locus_tag="OO130_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
BASE COUNT         4312 a         4226 c         4422 g         4148 t
ORIGIN      
        1 catcaaatag caaaatatac gctgcgagca attgcgtcgc aacaccacac cattcataca
       61 ctgtttgggt tgcttcaaaa tacttatcct ggctatccat aagtaaggtt ttaatatcgc
      121 cgactaaaac ggaggctgcc tgtgaatact cctgtttaaa gcgacttaac tcttttatgg
      181 tttcatcaaa ggtctgccag gggatgacct gatcgagata tttattataa agatctaatg
      241 ctccatctgc ggtttcgatt gcgtttttaa ctacttctac cattttatct gcaacgattt
      301 cagtcataat cattcgcctc tttaaatata taaattgtaa tgaaactcct gttttacaac
      361 tattaataaa ttttacttca tctaattcat agttagccgg gtgggatgcg tcaatgtctt
      421 tatttctatt aatatgataa atatcaaaca atgtttaatg tcattatggc gaatgcttct
      481 attctatttt tttgccgggt gatatttttc atttctgctg gatgagcgtc gtcgccagaa
      541 ggccacgtga gcacaggata agagaacgaa aaatcagcag tctatacagc gacaaatatt
      601 gatagcctga atcagtattg atctgctggc aagaatagac tactgtatat aaaaacagta
      661 taacttcagg cagattatta tgttgtttat caagcctgcg gatctccgcg aaattgtgac
      721 ttttccgcta tttagcgatc ttgttcagtg tggctttcct tcaccggcag cagattacgt
      781 cgaacagcgc atcgatctga atcaactgtt gatccagcat cccagcgcga cttacttcgt
      841 caaagcaagt ggtgattcta tgattgatgg tggaattagt gacggtgatt tactgattgt
      901 cgatagcgct attaccgcca gccatggtga tattgtcatc gctgctgttg acggcgagtt
      961 tacggtgaaa aaattgcaac tacgcccgac ggtacagctt attcccatga acagtgcgta
     1021 ctcgcccatt accatcagta gcgaagatac gctggatgtc tttggtgtgg tgatccacgt
     1081 cgttaaggcg atgcgctgat gtttgctctc tgtgatgtaa acgcgtttta tgccagctgt
     1141 gagacggtgt ttcgccctga tttatggggt aaaccggtgg ttgtactatc gaataatgac
     1201 ggttgcgtta tcgcccgaaa cgctgaggca aaggcgctgg gtgttaaaat gggcgatccc
     1261 tggttcaaac aaaaagatct gtttcgtcgc tgtggtgtgg tttgctttag cagcaattat
     1321 gagctttacg cagacatgag caatcgggtg atgtcgacgc tggaagaact atcgccccgc
     1381 gtcgagattt acagtattga tgaggcattc tgcgatctga caggtgtgcg taattgtcgc
     1441 gatctgactg attttggcag agaaattcgc gcaacggtgc tacaacgtac ccatcttact
     1501 gttggtgtgg ggatcgccca gaccaaaacg ctggctaagc ttgccaatca tgcggcaaaa
     1561 aaatggcagc ggcagacggg ggggtggtgg atttatcaaa tctggaacgc cagcgtaaat
     1621 taatgtctgc tctccccgtg gatgacgtct gggggattgg acggcggatc agcaaaaaac
     1681 tggacgcgat ggggatcaaa accgttcttg atttggcgga tacagatatc cggtttatcc
     1741 gtaaacattt taatgtcgtg ctcgaaagaa cggtgcgtga actgcgcggt gaaccctgtt
     1801 tgcaactgga agaatttgca ccgacaaagc aggaaattat ctgttcccgc tcgtttggtg
     1861 aacgtatcac ggattatacg tcgatgcggc aggccatttg tagttacgct gcccgggcgg
     1921 cggaaaaact tcgcagtgag catcaatatt gtcggtttat ctccacattt attaagacgt
     1981 caccatttgc gctcaatgaa ccttattacg gcaatagcgc gtcggtaaaa ctgctgacgc
     2041 ccactcagga cagcagggat atcattaacg ccgctacgcg atctctggat gccatctggc
     2101 aagcgggcca tcgctaccaa aaagcgggcg tgatgctggg ggatttcttc agtcagggag
     2161 tcgcgcagct caatttattc gatgacaacg caccgcgccc cgggagtgag caattgatga
     2221 cggtaatgga tacactgaat gctaaagagg gcagaggaac actctatttt gccgggcagg
     2281 ggatccaaca acaatggcag atgaagcgag ccatgctttc accacgttat acaacgcgaa
     2341 gttctgattt actgcgggtc aaataaatat agcggcagga aaaaagtgct cccgcaggag
     2401 cgccgaatgg attagcgacc gaacagatca cgttttttcg ctttaaacgg ctgggaaatc
     2461 atcaccagca ccgcgacaat caggtaagcg ataaaaatac cgagcagcca ctgcggcatt
     2521 tccagaccta aaaaatccca ctgacgctcg gcgcaatcgc cagaggcgac aaacacttgc
     2581 ggcacccatt tatccagcgg cagccattcc gggaaacgaa ccataaaatc acaggtggca
     2641 aacggcgaag gatagagctg aagcatggtg tgctcgtaag ttaactgcac accgcggaac
     2701 gcactataca accagataac catcgctaca taacgcagcg gagttttcgg ggcgatcgcg
     2761 ccaatcagcg cagcacccag aacgccgaat aacgcgcagc gttcataaat acagagcacg
     2821 caaggtttca gtaacatcac atgctggaac cacagcgccg tcagttccag tgccagagca
     2881 gtaaacgcca tcaacagcca cgcgccccgg ccttgtgaac attggttcaa aaatcgcaac
     2941 ataatcattt ccctgcaata tgcatagagt gcgcagttta aaccaattca ttcgctgcgc
     3001 caccaggagg cagcaaaaat aggacgcaat tggacaatta tcgggcgaaa aggcaaaccg
     3061 ggcatttata tgcccggtaa gttgttatca aagcgttgct atccagccca tttgcataaa
     3121 ccattcggtt acaggggcaa gcgtaaactc gacgcagagc agcccgacga gtgtcaggac
     3181 gagggtgtaa ggcagggcca tccacaccat gcggccataa gagaggcgaa tcaatggcgc
     3241 gagtgcagag gtcagcagga acaggaacgc agcctgaccg ttcggcgtag cgacagaggg
     3301 cagattggta ccggtattaa tggcgaccgc cagcagctcg tattgcttca acgtgatagc
     3361 gccactttcc attgccgctt tcgcttcgtt gatataaatc gtccccacga agacgttatc
     3421 cgaaatggat gacagcagac cgttgaaaat atagaacaac gacagctgag catgttccga
     3481 tgcctgcaac acaaactgaa taatcggcga aaacagttgt tggtcgataa tcaccgcgac
     3541 taccgagaaa aacaccgtca acagtgcggt gaatggcaga gattcggtga aggctttacc
     3601 gatagcatgt tcatcggtga caccggtcaa tgatgtcgcc agaataatga ccgacaaacc
     3661 aatcaagcca acctccgcca gatgcaacgc cagcgcagtc accagccaga cgccgataat
     3721 cgcctggaca atcagacgga ttttatcctg acgggtacgc tggtggcggc tttgatcgtc
     3781 aaactgttgc aacacttcgc ggactttctc cggcagcgtt tcaccgtagc caaaccaacg
     3841 cagcttctct accagcaggc aggttaacag gccacaaatc agaaccggaa cggtcaccgg
     3901 cgacatgcgc aggaagaaat cgccaaaatg ccagccagcc gctttagcga tgatcaggtt
     3961 ctgtggttcg cccaccatgg tcattacgcc gcctaatgcg gtgccgacac cggcatgcat
     4021 catcaggcta cgcagaaagc cacggaactg ttccagaacc actttgtaat gcttgtcgat
     4081 atgactatcg tcttgcaggt cggtatcttc agtacgggaa gaggctacgc ggtgataaat
     4141 accataaaaa ccgactgcaa cgctgatcac caccgccacg acggttaagg catcgaggaa
     4201 cgcggagagg aacgccgccg ccacgcaaaa agagagcgac agcagcattt tggaacgaat
     4261 gcttaacagc aaacgggtaa atatgaacag caatagctgt ttcataaaat agatacccgc
     4321 caccataaac atcagtaaca gcaagacttc aagatttgcc gccacctctt cacggacgtg
     4381 ttccgcgctg gtcatgccga tgaataccgc ttcaatagcc aacagaccac cggggagtag
     4441 cgggtagcat ttcagggcca tcgccagagt gaaaataaat tccgctacca gcaaccagcc
     4501 agcgacgaaa gggctgatga ggaaaattaa cggatttacg attaagaaaa tgatgagggc
     4561 gagtttgtac cagtcggggg actggcccaa aaaattgcgc catagcgcgc ggccccagga
     4621 gatctccatg atggtttccc ttaccttaca aataatcaat gatgttttta tgtttaaacg
     4681 caaagcttaa cggtcaggca ggagtgaggc aagtcttgat agtcaagggg aaagagatgc
     4741 ggaaaatgaa gccttgatcc cttttttctt ctttttgtct gctatcagcg tagttagccc
     4801 tctggtatga tgagtccaac tttgttttgc tgtgttatgg aaatctcact atggtcatta
     4861 aggcgcaaag cccggcgggt ttcgcggaag agtacattat tgaaagtatc tggaataacc
     4921 gcttccctcc cgggactatt ttgcccgcag aacgtgaact ttcagaatta attggcgtaa
     4981 cgcgtactac gttacgtgaa gtgttacagc gtctggcacg agatggctgg ttgaccattc
     5041 aacatggcaa gccgacgaag gtgaataatt tctgggaaac ctctggttta aatatccttg
     5101 aaacactggc gcgactggat cacgaaagtg tgccgcagct tattgataat ttgctgtcgg
     5161 tgcgtaccaa tatttccact atttttattc gcaccgcgtt tcgtcagcat cccgataaag
     5221 cgcaggaagt gctggctacc gctaatgaag tggccgatca cgccgatgcc tttgccgagc
     5281 tggattacaa catattccgc ggcctggcgt ttgcttccgg caacccgatt tacggtctga
     5341 tccttaacgg gatgaaaggg ctgtatacgc gcattggtcg tcactatttc gccaatccgg
     5401 aagcgcgcag tctggcgctg ggcttctacc acaaactgtc ggcgttgtgc agtgaaggcg
     5461 cgcacgatca ggtgtacgaa acagtgcgtc gctatgggca tgagagtggc gagatttggc
     5521 accggatgca gaaaaatctg ccgggtgatt tagccattca ggggcgataa tcccttccgt
     5581 ttaaaaagca aacccctcaa acgaggggtt ttttgttgtt tttacagatt tcccattctt
     5641 ggcgggcaac gttccagcaa ctcgacgctg ccatcttcgt tttgctgttc cagcatcaca
     5701 tcaaatcccc acaggcgatg cacatgcttc agcacttctt tgcgcccccg atccagcggt
     5761 gcgcgattat gtggaatgta acgcagcgtc agcgaacggt cgccgcgcaa atccacgttc
     5821 cagatctgaa tattcggctc cagattactt aagttatatt gcgacgataa ccggttacgg
     5881 atctcccgat aaccttcttc attatgaata gcggaaatct ccagataatt atgccgatcg
     5941 tcatccagca cggtgaagaa gcggaaatca cgcatcactt tcggtgacag aaactggctg
     6001 ataaaactct catctttgaa atcacgcatc gcgaaatgca gcgtttccag ccagtcggaa
     6061 ccggcgatat ccgggaacca gtatttgtct tcttccgttg gcgactgaca aatccgttta
     6121 atatcctgga acatggcgaa cccgagggca tacgggttga tgccgctgta ccacgggctg
     6181 ttataggggg gctggaagac cacattggtg tggctgtgca aaaactccag cataaaacgt
     6241 tccgttactt tcccttcatc atacagatgg ttaaggatgg tgtagtgcca gaaggtggcc
     6301 cagccttcgt tcatcacctg agtctgtttt tgcggataaa aatactggct caccttacgc
     6361 acaatacgca ggatttcacg ctgccatgat tccagcagtg gcgcattttt ctccataaaa
     6421 tagagcaggt tttcttgtgg ttcggacgga tagcggcgcg cttcagcaac cgttttctct
     6481 tcctcgcgct tcggcagggt acgccagagc atattgacct gactttgtag atactcttcg
     6541 cgacttttct gccgggcttt ctcttcttgc agcgagattt tttgcgggcg tttgtaccgg
     6601 tccacgccgt agttcatcag cgcgtggcac gagtccagaa gccgttctac ttcatccacg
     6661 ccataacgtt cttcgcactc ggtaatatat ttacgggcaa aaatcagata atcgacaatc
     6721 gaactggcgt cggtccaact gcggaataag taattgtttt taaagaaaga gttatgccca
     6781 tagcaggcgt gtgccatcac cagcgcttgc atggtaatgg tgttctcttc catcaggtaa
     6841 gcgatacacg ggttagagtt aatgacgatt tcataggcca gtccttgctg accgtgctta
     6901 tacagccgtt cagtctcgat aaacttttta ccgaatgacc agtgcggata gttaattggc
     6961 atgccgacgc tggagtaggc atccatcatc tgttctgagg ttatcacttc aatctggtgc
     7021 gggtaggtat ccagccggta gagtttcgcc acccggtcta tctctgccag ataaacatcc
     7081 agcaggtcga acgtccagtc gggtccatcg ctcaaccgtg tggtgtcctt attcatagaa
     7141 tcgatcgtcg ccatacgcgc acctcattgt tgtcggcgct ctctgtgtgg agcacctcat
     7201 ttcaagcata gaacacctgt taaaaaccgc gtcgccggag aatttttttc tttgcgattt
     7261 cttattatca gagtgccact aatccgcttc tgaacggaat tttatgctgg ataaaaaggg
     7321 cgttcagcag gagatactaa agacgccata ttgccgcaga gtcagggaga tgtgagccag
     7381 ctcaccataa aaaagctgta tgttgaataa tattttcaac tgagttatca agatgtaatt
     7441 agattattat tcttttactg tatctaccgt tatcggagtg gctatgcgag ttgtcatact
     7501 gggaagtggt gtggtaggcg ttgccagcgc ctggtactta aatcaggcag gacatgaggt
     7561 caccgtcatt gatcgggagc cgggggccgc cctggaaacc agcgccgcga atgccgggca
     7621 aatctccccc ggatatgctg caccgtgggc ggcaccaggt gtgcctttaa aagcgatcaa
     7681 atggatgttc cagcgccatg cgccgctggc ggttcgtctc gacggtacgc agtttcagtt
     7741 gaaatggatg tggcaaatgt tacgtaactg cgacaccagc cactacatgg aaaacaaagg
     7801 gcggatggtg cgtctggcgg aatacagccg tgattgcctg aaagcattgc gcgccgaaac
     7861 caatattcag tatgaagggc gtcagggtgg gacgctgcaa ctgttccgta ccgaacaaca
     7921 gtatgaaaat gcgacccgcg atatcgccgt gctggaagat gccggcgtac cgtatcagct
     7981 gctggaatcc agccgcctgg cggaagtgga gccagcgctg gcagaagtag cgcacaaact
     8041 gacgggtggt ctgcaactgc ccaatgatga aaccggagac tgccagctgt ttacccagaa
     8101 tctggcgcgg atggcagagc aggcgggggt taaattccgc tttaatacgc ccgttgacca
     8161 actgctttgc gacggcgagc aaatctacgg cgtgaagtgt ggcgatgaag tgattaaggc
     8221 cgatgcgtat gtgatggcgt ttggttctta ctcgacggcg atgctcaaag gcattgttga
     8281 tattccggtg tatccgctga aaggctactc gctgactatt ccaattgcgc aggaagatgg
     8341 tgcgccagta tccaccattc ttgatgaaac ctacaaaatc gccattaccc gtttcgataa
     8401 ccgcattcgt gttggcggaa tggcggaaat tgttggtttt aacaccgagc tgttgcaacc
     8461 gcgtcgtgaa acgctggaaa tggtggttcg cgatctctat ccacgcggcg gtcatgtcga
     8521 gcaggcgact ttctggacgg gtttgcgccc gatgacgcca gacggtacgc cggttgtcgg
     8581 gcgtacacgc tttaaaaatc tgtggctgaa taccggtcac ggcacgctcg gctggacgat
     8641 ggcttgcggt tccggtcagt tgttaagcga tctgctctct ggtcgcacgc cagcgatccc
     8701 atatgaggat ctaagcgtag cgcgctacag ccgtggattt acgccatcac gtccgggcca
     8761 tttacatggc gcacacagct aaggaaacga gatgacccgt ccgatacagg ccagcctcga
     8821 tctgcaggca ttaaaacaga atctgtccat tgtccgccag gccgcgccgc acgcgggtgg
     8881 taaaagcgaa cgcttacggg catggtattg agcgtatctg gagcgcgctc ggggccaccg
     8941 atggctttgc attacttaac ctggaagagg caataacgtt acgtgagcgc ggctggaagg
     9001 ggccgatcct gatgctggaa ggatttttcc atgctcagga tctggagatt tatgaccagc
     9061 accgcctgac cacctgcgta cacagcaact ggcagctcaa agcactgcaa aatgcgcggc
     9121 taaaagcacc gttggatatt tatcttaaag tgaacagtgg gatgaatcgg ttgggcttcc
     9181 agcccgatcg cgtgcttacc gtctggcagc agttgcgggc aatggcgaat gttggcgaga
     9241 tgaccctgat gtcgcatttt gccgaagcgg aacatcctga tggaatttcc agcgcgatgg
     9301 cgcgtattga gcaggcggcg gaagggctgg agtgtcggcg ttcgttgtcc aattcggcgg
     9361 cgactctgtg gcacccggaa gcgcattttg actgggttcg gcctggcatt attttgtatg
     9421 gcgcttcgcc gtccggtcag tggcgtgata tcgccaatac cggattacgt ccggtgatga
     9481 cgctaagcag tgagattatt ggtgtccaga cgctaaaagc gggtgagcgt gtgggctacg
     9541 gcggtcgcta tactgcgcgc gatgaacagc gaatcggcat tgtcgccgca gggtacgccg
     9601 acggttatcc gcgccacgcg cctaccggta cccctgtttt agtggacggc gtgcgcacca
     9661 tgacggtggg gaccgtctcg atggatatgc tggcggtcga tttaacgcct tgcccgcagg
     9721 cgggtattgg tacgccggtt gagctgtggg gcaaggagat caaaattgat gatgtcgccg
     9781 ccgctgccgg aacggtgggc tatgagttga tgtgcgcgct ggcgctacgc gtcccggttg
     9841 tgacggtgta acttgttgta agccggatcg gaggcaacgt cttctgggtg caaaaaaaat
     9901 catccatccg gctggtcagc aactgtagtt gttaatgtga cagagccatt gcccatgata
     9961 gtgtccatta aaaggatgga cactatttcc ccggaacctg aactcaccgc acaggcgttc
    10021 tacataaaac gcttacgctt cattgttgac tcggagtcga cttcagataa atcacgcttt
    10081 acccttgatg gagcctgtac atagatttgt gtaattgcct gattttgata tgttcaattc
    10141 aacatcaaat gaaggttaaa ttatggacga caaacaattg caggctcagg ctgcgttcag
    10201 caaagcatcg caaccggcga tagatgcttc attaaattta agattcagct tcctcttcag
    10261 ccacccgtac gccaatcttc aacacttcat tatcttcttt ctcggccacc gtccagatca
    10321 tcccggcaaa ctctacctgg tcaccgacaa ccggtgccgt gcctaacaac tgctggacaa
    10381 tttcacccag cgtttgctgc ttatcacgat attctcgccc gtcttccagg ccatatatca
    10441 gcgcaacatc agcatatttg gcgctggctt cgagaatgaa gtcaccaaag aagcgttgat
    10501 ccagcgcgac cggcggcgac tggctgaaca gtttaccgag cgccggaaga tcgcgttccc
    10561 gaccaattac acacaacaca tcgccttcgc gcagtcgggt gctgccggta ggatgaagca
    10621 actggttatc acgaaacagt gccgcaatac gcgtctcttt tggcatatgc aaatcgcgca
    10681 gtgccgcgcc cacgcaccat ttatcggcac tcaattgata aacaaactgc tcccacggat
    10741 tttccggatg aatatccagg ccaacgcgtg acaccggacg tcccactggc ggaaccacca
    10801 ctttggcttt tttagccgcc cacgagagtg atgttccctg caagagcagt gaaaccagaa
    10861 ccacaaagaa ggcgacatta aagaacagac gtgcattctc cagccccgcc atcatcggga
    10921 acacggccag gatgattggc accgcgccgc gtaatcctac ccagctgata aacacgcgct
    10981 cacgcagatt gaaaccgcgg aaggggagca atccagcaaa taccgaaagg ggacgggcga
    11041 agaatatcat ccatgccgac aaaattagcg ccggaatggc aataggcagc agatcgcttg
    11101 ggttaaccaa tagccccagc accaggaaca tggcgatttg cgccagccag gcgaggccgt
    11161 cgaaattttg caggatgccg tagcggttgc gaatcggccg attacccagc agaaaaccgc
    11221 acagataaac agccagaata ccgctgcctt ccagcgccgt ggttaaagca aaaattaaaa
    11281 taccgccgct taatgccagc aatggatata atccggcagg cagggcgatg cgattaatca
    11341 tttgcagcag taaataacca ccgccaagtc cgatgacaat cccgaggcca aattgctgca
    11401 gaatatcgac cacgaacatc caactgacac tgctttcatg ttgctggatc atcgcaatca
    11461 gggtaatcgt cagaaagacc gccattggat cattactgcc ggattcaatt tccagcgtcg
    11521 agccaacacg ttcgttaagc cccttaccac ccagcagaga aaagaccgct gcagcatcgg
    11581 tagagccgac gatagcgccg attaataagc cttcaatcaa atcaagatta aacagccacg
    11641 ccgccatcat gccggttaaa ccagaggtga taagcacgcc cagcgtcgcc agcgacagtg
    11701 ccggtcctaa cgccacacga aaggaactgg cctgagtgcg catcccgccg tcgagcaaaa
    11761 taatcgccag tgccaggtta cttaccatgt aggcgaaggg gtaattatca aacgggatac
    11821 cgccgacgcc atcgactcct gccagcatgc cgatcgccag aaaaataacc agaataggaa
    11881 tgccaagacg ggaagaaaat gaactaagta atatactgct ggtgacaagg atggatccta
    11941 agatgaaaag gctaattatt gttgtggcat ccaacggtcg gttactcctg attacgctgt
    12001 ctcttatata aaccctacca tattagcggc agagacagcg ttttacttag ccctgaagcg
    12061 tgattttttt acattttaag aacaggatga ccgctaatcg tcaactgagt gccttcccgg
    12121 gtgttattca gaatggcatg tgcgcccaac ggcagcgtaa ccgtgcgttg ttcatgacca
    12181 aaatcgaggc cggtaatgag tggaatcgac aggcgggaac gcaaaaacgc ggacactgac
    12241 tccaggttgt aacctgcgtc ataatcattg ggcgtgctgc cgctaaagct accgagaata
    12301 atcgccttct gacgtggcaa aattcccgca tgatagagct gcaacagcat acgttcgacg
    12361 cggaaagggt gctcgttaat atcttccagt accaggatac cgttctcaat ttttggcatc
    12421 cacggtgtac caatcagtga aatcagcatc gcaagattgc ctccccacaa cgtgccttcg
    12481 gtctgacatg tcgggccttc cccttgccat tcaatggtga aggttttatt gcgtaacgcc
    12541 agccagaaat ggtgctcggt aaaggcgttc agttcatccg caccaaagtt tgctaccagc
    12601 atggggccgc taaaggtgat gacattgcct tgcgccagaa gaccgcactg aatggcggta
    12661 aaatcgctat gtccgcaaat gagtaacggg tcatgttgtt ggcgagccac cagtgcctgc
    12721 cagtcaatat ctgccagtaa acgactggca ccgtaaccgc cgcgtacagc cagcacgatg
    12781 gtgttgggtg tagtcagtct ggcaagggaa ttgagatctt ccagacgctc cgtttccgta
    12841 cccgcaaaac gctcacaacg acgggcaata acctcgacgt tattgacttg atgccccgcg
    12901 tcggtaaggc gctggatacc acgcagcgcg gcgtgctgtt taatgctgta acccgatggg
    12961 gcaattaagt gaaacagaga catggcaatt ccttgctgac aacagaaacg aaatgtatat
    13021 catgccgctt aggtgtgccg ttgtcacctc aacggcgatt ccaggctata aggatagaag
    13081 aagtgaaatt gagatggttt gcctttttga ttgtgttatt agcgggttgt tcatcaaagc
    13141 atgactatac gaacccgccg tggaacgcga aagttccggt gcaacgtgcg atgcagtgga
    13201 tgccaataag ccagaaagcc ggtgcagcct ggggcgtcga tccacaattg atcacggcga
    13261 ttatcgctat cgaatcgggt ggtaatccga acgcggtgag taaatcgaat gccattggtt
    13321 tgatgcagtt aaaagcttca acctccggac gtgatgttta tcgccgtatg ggctggagtg
    13381 gtgagccgac gaccagcgag ctgaaaaatc cagagcgtaa tatttcaatg ggggcggctt
    13441 acctgaatat tctggaaacc ggcccgctgg caggcattga agatccgaag gtactgcaat
    13501 atgcgctggt ggtgtcatac gctaacgggg caggtgcgct gctacggact ttctcgtcag
    13561 atcggaaaaa ggcgatcagc aaaatcaacg atttggatgc tgacgagttc ctcgatcacg
    13621 tagcgcgcaa tcatcctgcg ccgcaggctc cgcgctatat ctacaaactt gagcaggcac
    13681 tggacgcgat gtaaatcagt cgcgtacttt gtccgctttc tcccgggctt cccgctcgag
    13741 agagaaaata atccgttgta attgccgctc caccgtcggg ctgacgttaa gaaaacggaa
    13801 gctcagacgg ggagtggtga tggtttcatt cttgccatca atcactttgc gctcgctgat
    13861 ggagattaac tgggcgtcaa agtgaaaaac accccattgc cccatgttga cttcaatctg
    13921 agcgaagcgc atgccttctt gtaattcggc aggctttgct gtttccagta atgcgcccat
    13981 gccgcctaac gacaaatcat acaggcggaa acgtaacgta ctgttatccg ccagtttggt
    14041 ctggcaaaaa taaggcggat ggagtggggc ggagatgcgg aaatatcggc gtcgttgtac
    14101 aaaccataag gtgggaggcg gtacggtaat aaatgccgga agctgcaagt attcactctg
    14161 ctgtagttgt tcaacagtaa actcgacttt cgcaccctga gtttcggcgg taatggtaat
    14221 atgctgcgcc tttagcacgg cgatgttgtc ttcggcttga ctgccgaaat ccagcaccag
    14281 tttatccggg gttattgcca gtattttgct gatcagctgc ccgccattcc aactgagacg
    14341 caaaggaatt gcggctttgt gcaaatcgcg taacacgccc aggacggcta acggattttg
    14401 tttcaggaac tgctcatggt aatgactcac gcggaaaaac tcctgactga caaactgtct
    14461 ttggtttatc ggtcacagtt aacaaaactt aatacaaatg cgtgaatatt ttttacatgt
    14521 tgttcttaaa tcagccgaca tacgcccagc atcgatcccg cccttgccta tacttagagc
    14581 gttgacgtaa gcatttcttg cgtccgattc atcgaaacga gggcttgaac atgggaatta
    14641 ttgcctggat tatttttggc ctgatagccg gcattatcgc caagctaatc atgccggggc
    14701 gtgatggtgg tggatttttc cagacctgta ttctcgggat agtcggtgcg gtggtcggcg
    14761 gctggctggc gaccatgttt ggcattggcg gctccatcag cggttttaat ttgcacagct
    14821 tcctggtggc ggtggtggga gctattctcg ttctgggctt attccgcctg ctgcgaagag
    14881 aataagattt tcataaggcg gatagcgata cagatgccgc tatccgcttt tcacatcaga
    14941 acgtatattc gacacctgct ttacgggtga aaaaaatcaa tatagcactt cgatatccga
    15001 taaagtcggt tcccggtaac aacaacagaa tcctttttta attattgttt cgttgttttt
    15061 ggtgtgattc agaaaataaa tattttagac agtagccagt caggcgttaa aaatggggcg
    15121 gatagtgagg cggttttggc tgcgcttatt gaacatttag aattgatcaa tccttctggc
    15181 agattaagcc aaaatacccg ttcggcaatg cttcagttac gcgaagagga atggtctgaa
    15241 ttcttttttt ggttgctaaa ttctctggag tgtctggatt atgtgattat taatttaact
    15301 ccagaaagca aaaaaacgtt gatgagtgaa caccgtaaca atattcaggt agcaatcgat
    15361 gctttatata ggcaacgtcg gtgtaaatca ccgggggatg atagtgaaac acttacccgt
    15421 agaaatgacg ctatcttcgg caaccacgta tggcaaacat ttgcgcaata cttcccgcca
    15481 ggacttgaaa aaccggctgt gtagaaaaca ccatagctat ggtgttatct gccgacaacg
    15541 ccatccggag aagccgaatg acgttgtcgg agtaagcgct ggttaaggtg tgggttgtgc
    15601 ctctttggtt gagggttgcg tcgttgctga cttaacggtc ggacgcgtcg ccggaacatt
    15661 gtcacacggt tgctctttcg ggcagatcaa atccagcatt ttcagcgtca cgccattggt
    15721 ccagccaaag ccatcctgta atggatattc gccacctctt tttgcccgta gttttgtaat
    15781 ccttctgtcg cgacccactg taacggtgcc cagccatttg gcgcatccca ttgttgccca
    15841 cttttcaccg acgtggtgtt caggccgccg ggttgcagca gatgtgtttt cgtcgccgtc
    15901 gccattttgt tggcgcgatc tttcgctgcc gcattgacgt acagcgggaa cagggcggcc
    15961 gcggttaact gattgcgcac tttatgactt ttcaggtcgt aatcggcata ccagccttgt
    16021 tgatcgttcc acaggtattt ttcgattcct ttttgacggg cattcgccag cgtttcgtac
    16081 tggtttgcca tcgcgttatc tcctgcagct ttgctggcgc gggcgaggat tttttccatt
    16141 ttaaacatca ggctgttcag atcgaccggt acgatgctgg tggtgcgtaa ggtatttaac
    16201 tgctgcgggt tgtccatcca gcgcgagctg aaatcccagc cagacgcagc ggcagagcgc
    16261 aggtcgcggt aaatttcagt ggcaggtcga ttcggattgc ttttggcggt ggcaatatct
    16321 tccacccatg actctggtcg tggcgtatcg cgatcgtccc agtagcggtt gagaagggta
    16381 ccatcctgaa gtttgacaac gcgtttttcc tgttgtccgg cttgcaggtt ttcaacaccg
    16441 tccatccagt aagcatattc tttttgcatt tgcggcaggt attgcttcaa cgcggcatcg
    16501 ccttcatgct gcgccagtaa ctctaccatc agggcaaaga agggcggttg cgagcggctt
    16561 aaatagtaac tgcggttgcc gttgggaata tgaccgtaag tgtctatttc atgagcaaaa
    16621 ttggccacca tatccgcgac tttatcccag tgaccgcttt cagcaagtcc taacatggtg
    16681 aagtaactgt cccagtaata tacctcgcga aaacgtccgc ccggcacgac ataaggttcc
    16741 ggcagcggta acagagaatc ccatttttcg gtgttttcgg tagaacgcgt taataccggc
    16801 caaagtccgt caatatgttc gcgcagtgac tgcccctctg gcggaacata tttctcgcct
    16861 tctttcggca gggtgaaatt gacgttaacg aaatggcgca gatcaaatcc gctctggttt
    16921 tgctgcatcc gataatcagc ggatcgctgt tcggcacggc atcggcaaag gttttttggt
    16981 ccggaaaaag tttggcgttt tgcacatcat taaacagcgg ccctaataaa atatcaggcg
    17041 gctatggtgt taccggtgtt tcttctgcct gcaccgatag cgcagcgaaa cacaaaaaga
    17101 tacaggct
//
LOCUS       DAJTMC010000083        17011 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.083, whole genome shotgun sequence.
ACCESSION   DAJTMC010000083 DAJTMC010000000
VERSION     DAJTMC010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17011
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.083"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            177..614
                     /locus_tag="OO130_002429"
     CDS             177..614
                     /locus_tag="OO130_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HCR5365804.1"
                     /translation="MKTIKPESIALFCLLSGDVDLAKHLADILPITCFVGEDWQQDFQ
                     PLDGGPVQSLSDSYSDYSVLLLIAAESEISVPEWLAHSEGTTVILIAEHNARLLTGPA
                     GGAVALIHYLNEICHSRETAVNDAFCRTLTRGSLAIPQCHLLS"
     gene            748..1292
                     /gene="cobU"
                     /locus_tag="OO130_002430"
                     /pseudo
     CDS             748..1292
                     /gene="cobU"
                     /locus_tag="OO130_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
     gene            1289..2032
                     /gene="cobS"
                     /locus_tag="OO130_002431"
     CDS             1289..2032
                     /gene="cobS"
                     /locus_tag="OO130_002431"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HCR5365805.1"
                     /translation="MSKLFWTMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGVPLAALFSVLVLALMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILALLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            2044..3123
                     /gene="cobT"
                     /locus_tag="OO130_002432"
     CDS             2044..3123
                     /gene="cobT"
                     /locus_tag="OO130_002432"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HCR5365806.1"
                     /translation="MQTLADLLNTIPAIDPAAMSRAQRHIDGLLKPVGSLGRLEALAI
                     QLAGMPGLNGVPHVGKKAVLVMCADHGVWEEGGAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVYVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRHQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRAPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGMAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMEMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            3185..4120
                     /gene="ldtA"
                     /locus_tag="OO130_002433"
     CDS             3185..4120
                     /gene="ldtA"
                     /locus_tag="OO130_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR5365807.1"
                     /translation="MMRRVNIFCSFALLFASHTSLAVTYPLPPEGSRLVGQSYTVTVP
                     DHNTQPLETFAAQNGQGLSNMLEANPGADVFLPKSGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSNWLEVHEPLSRNRAEYEFDRKVPLPVTPSLR
                     AFISGQEVDVNRANAALQRRSGMPVPISSGSRQMF"
     gene            complement(4176..4251)
                     /locus_tag="OO130_002434"
     tRNA            complement(4176..4251)
                     /locus_tag="OO130_002434"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4216..4218),aa:Asn,seq:gtt)
     gene            4578..4949
                     /locus_tag="OO130_002435"
                     /pseudo
     CDS             4578..4949
                     /locus_tag="OO130_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416493.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            4948..5382
                     /locus_tag="OO130_002436"
                     /pseudo
     CDS             4948..5382
                     /locus_tag="OO130_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Catalyzes the hydrolysis of AMP to form adenine and
                     ribose 5-phosphate using water as the nucleophile;
                     incomplete; partial in the middle of a contig; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
     gene            5725..6441
                     /locus_tag="OO130_002437"
     CDS             5725..6441
                     /locus_tag="OO130_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR5365808.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGSDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
     gene            complement(6897..6972)
                     /locus_tag="OO130_002438"
     tRNA            complement(6897..6972)
                     /locus_tag="OO130_002438"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6937..6939),aa:Asn,seq:gtt)
     gene            complement(7073..8716)
                     /gene="yeeO"
                     /locus_tag="OO130_002439"
     CDS             complement(7073..8716)
                     /gene="yeeO"
                     /locus_tag="OO130_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HCR5365809.1"
                     /translation="MLRHILTAKNLLSNPIFKFPNCLPFLSTVCCICRQFVGENLCSF
                     ADSPSLFEMWFHFLQLRSALNISSALRQVVHGTRWHAKRKSYKVLFWREITPLAVPIF
                     MENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVVVAFSLG
                     KRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKVLALTYLEL
                     TVLSYPAAAITLIGSGALRGAGNTKIPLLINGSLNILNIIISGILIYGLFSWPGLGFV
                     GAGLGLTISRYIGAVAILWVLAIGFNPALRISLKSYFKPLNFSIIWEVMGIGIPASVE
                     SVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITGRRLGVG
                     QIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGVMASFYTQDPQVKHVVVILIWLNALF
                     MPIWSASWGLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLGIMLGWGVVGVWMGMF
                     ADWAVRAVLFYWRMVTGRWLWKYPRSEPQKCEKKPVVSE"
     gene            8721..8796
                     /locus_tag="OO130_002440"
     tRNA            8721..8796
                     /locus_tag="OO130_002440"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8754..8756,aa:Asn,seq:gtt)
     gene            8958..10220
                     /locus_tag="OO130_002441"
     CDS             8958..10220
                     /locus_tag="OO130_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCR5365810.1"
                     /translation="MSLNDSKIRNLKPSSKPVKLSDSHGLYLLVNQGGSRIWYLKYRF
                     SGKESRVSLGAYPLVSLAEARQRRDDIRKLLTQNINPAHQRMSDKAAASPEKYFKAVA
                     LAWHKTNKKWSPDYAARILASMENHIFPAIGHLPVTTLKTQNFTALLRVIEDKGFLEV
                     ASRTRQQLCNIMRYAVQQGFTENNLAQHLEGVTAPPVKNHYPALPLERLPELFERIGD
                     YQQGRQLTRLAVVLTLHLFIRSSELRFARWGEIDFRNKIWTIPATREAIEKVRFSGRG
                     AKMRTPYIVPLSRQAIAILKQIKEISGHLELVFPGDHNPYKPMSENTVNRALRLMGYD
                     TKTDVCGHGFRTMACSALVETELWSRDTVERQMSHQERNSVRAAYIHRAEHLDARTAM
                     MQWWSDYLDVSREGYVAPYIYARRHKAA"
     gene            complement(10932..11816)
                     /locus_tag="OO130_002442"
     CDS             complement(10932..11816)
                     /locus_tag="OO130_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000511771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase domain-containing protein"
                     /protein_id="HCR5365811.1"
                     /translation="MGILEQEMKRLAQQAGGSHKTVHDRIKLVQRFCERLVLAQNVQI
                     RRVEQLKARYIEGYVRERLAQGITKRSLQNEMAAVRCILKQAGRDRLAQSERLNNRSL
                     GLSGASRNGTKRAITPEHYRYVLETARAKDPGMAAALELSRLMGLRSQEVVQSAQSLR
                     TWKQSLERGEPRLTVVFGTKGGRPRETVILDAVAIRKALDNALVVAEDRHGRLIDKPD
                     LKSAMKYWHSQASRLGLTGTYSPHSLRYAWAQDAIRHYLAQGFSEKEALAMTAMDLGH
                     GDGRGRYVAQVYGRKDTD"
     gene            12375..12521
                     /locus_tag="OO130_002443"
     CDS             12375..12521
                     /locus_tag="OO130_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5365812.1"
                     /translation="MYRIKTLASVAMFCDPVFAEVCTGKRIDRPDNRRFFQVVKRWAL
                     STTA"
     gene            complement(12681..12998)
                     /locus_tag="OO130_002444"
     CDS             complement(12681..12998)
                     /locus_tag="OO130_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016161430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCR5365813.1"
                     /translation="MDRGEIWLVSLDPTTGHEQSGKRPVLIVSKASFNTFTRLPVVVP
                     VTSGGNFAREAGFTVSLDGAGTKTTGVIRCDQPRTIDMGARSGKRLERIPDAVVNEVL
                     ARL"
     gene            complement(12999..13256)
                     /locus_tag="OO130_002445"
     CDS             complement(12999..13256)
                     /locus_tag="OO130_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000557619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HCR5365814.1"
                     /translation="MYTTRLKKVGGSVMLAVPPAVLKTLGLSTDSEVGMTIDNGCLII
                     EPQKRPRYSLEELLAQCDPHAEISEEGREWIDAPAVGKEIL"
     gene            complement(13605..13811)
                     /locus_tag="OO130_002446"
     CDS             complement(13605..13811)
                     /locus_tag="OO130_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003842122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5365815.1"
                     /translation="MMLQDWHPADIIAGLKKRGTSLSALSRQAGLASSTLANALTRRW
                     PKGERLIAEALGIKPEQIWPSRYR"
     gene            complement(13808..14269)
                     /locus_tag="OO130_002447"
     CDS             complement(13808..14269)
                     /locus_tag="OO130_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5365816.1"
                     /translation="MATIPTQTHPLLAVPFSAATDFTVLADHCENVAETLIESEDPVL
                     RLALCGRLAACLTLLQPTLNDPIPSHLTDSLTVDALPADMPRFVPDAELLCDYSLTLA
                     QLLIGQGMQPKVEQTITGLLCELVWYFAAEMKAPRWLRTADGVKFIDEVAA"
     gene            complement(14640..16244)
                     /locus_tag="OO130_002448"
     CDS             complement(14640..16244)
                     /locus_tag="OO130_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5365817.1"
                     /translation="MITKELVDESNFISQLVDIGRFDESYQQSVAFLNKLERITIRND
                     NYFIVLANIAGALVDIGQMQNNKNASELGCNLMEDNKEAFISVLGECLYYYNYGNALS
                     NLVSITNPNDHTFKSIEELVSLKNIYWRSFKLSAEEQEEFQAELSVNLANSLRSQFRL
                     SESLRYYDLTNLKGLDIPQAWVNRSMSLMVLNLISSSFSIKLLKEVRAGYIKASVSKN
                     IPPQWESFYLERIAQTNEKIAEYAVDDETDEHDEALTQQEFEVLSPYRQFCLRNHLTL
                     SEHGLYCPCVGSATDDLVIASRGGVTGDFIIPMEMVLNRLKSEFSLARHLYFDYLHPQ
                     NTDYIKYESHFLELYNDEVLGIEIEKIRTAFRLCFGILDKIAVAICELYNLYPTTKKG
                     TPQKNIYFQNFWQLDVDNRRQLFEKVKSPGLLALYSIATDLNKDKGGELAFYKEWRNG
                     LEHKFLVVHKSDKPEDIYESYQLIEDILFIKESDFIHHFEHLLQITRSAIFSFAFMVR
                     QEGMKEKKEGIHYMTRELHAINYSAD"
     gene            complement(16257..16622)
                     /locus_tag="OO130_002449"
     CDS             complement(16257..16622)
                     /locus_tag="OO130_002449"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5365818.1"
                     /translation="MVSDKRADIIVSLPQIKIPIEIKRDYHRDVWTALNGQLDKLYTK
                     NPDAAGHGIYLVFWFGSARPNSLPHLAKNTSQPENASAMENMLNETVPVDKRDRLSAI
                     VIDVSCEGIPPVEAPKSSV"
     gene            complement(16650..16859)
                     /locus_tag="OO130_002450"
     CDS             complement(16650..16859)
                     /locus_tag="OO130_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001044500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5365819.1"
                     /translation="MNKTPANIMDLYSLLLDHIRDISNRIANENTDIYKQFWNEDSYG
                     RTLTPKPEESCRNIFLDLLRVRLNP"
BASE COUNT         4479 a         4013 c         4080 g         4439 t
ORIGIN      
        1 aatttctttt tttcattgga gcacctgtgt tgttctgagg tgagcatatc acctctgttc
       61 aggtggccaa attcagtgtg ccacttcacc agggctgcat tcaaggtgtg gcaatattgt
      121 ttcatggctt atcaaccaga gcgccattac aggaaattaa gtggagggcg agaggcatga
      181 aaacgataaa acccgaatcg atagctcttt tttgcctctt atctggagat gtggatctgg
      241 cgaaacatct ggctgatata ttgccaataa cctgttttgt tggtgaggac tggcagcaag
      301 atttccagcc tcttgatggt gggcctgtgc aatcgttatc agattcgtat agcgattatt
      361 cagtgctgct attgatagca gctgaaagcg agatatccgt accagaatgg ctcgctcatt
      421 ctgagggaac gacggtaatt ttgattgcag aacacaatgc ccgtttgctg actggtccgg
      481 ctggtggggc tgtagcatta atccattatc tgaatgaaat ctgtcattcc agagaaaccg
      541 ctgtcaatga cgcattttgt cggacgttaa cacgaggaag tctggctatt ccgcaatgcc
      601 atcttctttc gtaatggatt gagaatgctg gatttttaat tatattacgc gcagtattaa
      661 tcgcgtaaat gttttgcggt cagcaacggt gggggatcca ttgttctgtt caatgaatac
      721 catcatacaa ttgaatcagg agtcggtatg atgatttcgg tgacgggggg catacggagc
      781 gggaagagtc gccacgcaga ggcgcttatt ggggactctt cacaggttct gtatacgcca
      841 cctcgcaaat ccttgatgat gagatggctg cacggataga acatcatcgg caaggccgcc
      901 cgacgcactg gcacacagtg gagcgctggc aacatcttga tgaattaatt catgcagaca
      961 ttaacccgca tgaggctgta ttgctggaat gcgttaccac catggtgact aatctgttgt
     1021 ttgattatgg cggcgataaa gaccctgatg aatgggatta tcaggcgatg gaacaggcga
     1081 ttaatgctga gattcagtcg ttgattgctg cctgccaacg ttgtcccgca aaggttgtat
     1141 tagtgactaa cgaagtggga atggggattg tgccggagag tcgtctggca cgacattttc
     1201 gtgatattgc cgggcgggta aatcagcagc tggccgctgc ggcaaatgaa gtatggttgg
     1261 tggtttcggg tattggagta aaaatcaaat gagtaaatta ttttggacga tgctctcttt
     1321 tattacgcgt ctgcctgtac cgcgtcgctg gtcccaggga ctggatttcg agcattattc
     1381 tcgcggtatt attacttttc ctttaattgg attattgctt ggcgcgatta gcgggctggt
     1441 cttcatggtg ctgcaggcat ggtgtggcgt accactggcg gcactgttta gcgtacttgt
     1501 gctggcgctg atgaccgggg gattccacct ggacggcctc gccgatacct gtgatggcgt
     1561 attttctgca cgtagccgcg atcgcatgtt ggaaatcatg cgtgatagcc gtttaggcac
     1621 ccacggcggt ctggcattga tttttgttgt actggcaaag attctggtat taagcgagtt
     1681 ggctctgcgt ggcgaaccga tccttgcatt gctggcggca gcatgtgcgg tcagccgcgg
     1741 gactgccgca ttattgatgt atcgtcatcg ttatgcccgg gaagaaggac ttggcaatgt
     1801 gttcattggc aaaattgatg ggcgacaaac ctgcgtcaca ctcggcttag ccgctatttt
     1861 tgctgccgta ttattgccgg gtatgcatgg tgtggctgct atggtggtga cgatggtggc
     1921 gattttcatc ctcggtcagc ttctcaaacg tacgctgggc gggcaaaccg gcgatacgct
     1981 gggagctgct atagagcttg gtgaactggt cttcctgctg gcactgctat aaagagacgt
     2041 gacattcaaa cacttgccga tttactgaat acgatccctg ctatcgatcc tgccgctatg
     2101 tcgcgtgcac aacggcatat cgacgggtta ctcaaacctg ttggtagcct gggaaggctg
     2161 gaggcgcttg ccatacaact ggcaggaatg ccggggttga atggcgttcc gcatgtgggc
     2221 aaaaaagcgg tactggtcat gtgtgccgat cacggcgtct gggaggaagg gggcgctatt
     2281 tccccaaaag aagtgacagc cattcaggct gaaaatatga cccgcggaac aaccggcgtg
     2341 tgtgtgctgg cagcacaagc gggcgctaac gtctacgtaa ttgatgttgg tattgatact
     2401 gctgagccta tccccgggct tatcaacatg cgtgttgctc gaggtagtgg caatattgct
     2461 tcagctccgg caatgagtcg ccatcaggca gaaaagttgc ttttggacgt catatgttat
     2521 acgcgggagc tggcaaaaaa cggtgttacg ctgtttggtg taggtgaact ggggatggca
     2581 aacacgacac cggcagcggc aatagtcagc acaatcactg gccgggctcc tgaagaagtg
     2641 gttgggattg gcgcaaacct gccgacagat aaactggcta ataaaattga tgttgtgcgc
     2701 cgggcgatta cgttgaatca accaaatccg caggatggcg ttgatgtcct ggcaaaagtg
     2761 ggtggatttg atttggtcgg aatggctggc gtgatgttag gcgctgcgtc ctgcggttta
     2821 cccgtgttgc tggatggatt tctttcttat gctgctgcgc tcgcagcctg tcagatgtct
     2881 cctgcaatca aaccgtatct cattccttct cacctgtcgg cagaaaaagg agcgcgtata
     2941 gcgctgtcgc atttggggct ggagccttat ctcaatatgg agatgcgttt aggtgagggg
     3001 agtggcgcag ctctggcgat gcccatcatc gaagctgcct gtgcgatata taacaacatg
     3061 ggcgaacttg ctgccagtaa tattgttcta ccggggaata cgacttctga tttgaacagt
     3121 taaaaacgaa aagtctgata aaagctatac ttaacggata gctttcgcga cttaggaaag
     3181 ggacatgatg cgtcgtgtaa atattttttg ctcatttgct ctgctttttg ccagccatac
     3241 tagcctggcg gtaacttacc cattacctcc agagggtagc cgtttagtgg ggcagtcgta
     3301 tactgtaact gttcctgatc acaataccca gccgctggag acttttgccg cacaaaacgg
     3361 gcaagggtta agtaacatgc tggaagcgaa cccgggtgct gatgttttct tgccgaagtc
     3421 cggctcgcaa ctcaccattc cgcagcaact gattttgccc gccactgttc gtaaagggat
     3481 tgtggttaac gtcgctgaga tgcgtcttta ttactaccca ccagacagta atactgtgga
     3541 agtctttcct attggtatcg gccaggctgg gcgagaaacc ccgcgtaact gggtgactac
     3601 cgttgaacgt aaacaagaag ctccaacctg gacgccaacg ccgaacactc ggcgcgaata
     3661 tgcgaaacga ggggagagtt tgcccgcatt tgttcctgcg ggccccgata atcccatggg
     3721 gctgtacgcg atttatattg gcaggttgta tgccatccat ggtaccaatg ccaattttgg
     3781 tattgggcta cgggtaagtc agggctgtat tcgtctgcgc aatgacgata tcaaatatct
     3841 gtttgataat gttcctgttg ggacgcgtgt gcaaattatc gaccagccag taaaatacac
     3901 cactgaacca gatggctcga actggctgga agttcatgag ccgttgtcgc gcaatcgtgc
     3961 agaatatgag tttgaccgaa aagtgccatt gccggtaacc ccatctttgc gggcgtttat
     4021 cagcgggcaa gaagttgatg taaatcgcgc aaatgctgcg ttgcaacgtc gatcgggaat
     4081 gcctgtgcca attagttctg gttcaagaca gatgttttaa gagcgttggt aattagaaag
     4141 caaaaagcct gctagaaagc aggctttttt gaatttggct cctctgactg gactcgaacc
     4201 agtgacatac ggattaacag tccgccgttc taccgactga actacagagg aatcgtgtga
     4261 acggggcgca tattaatggc agtgcatggt gatgtcaaag ggtgaaatgc atttttttgt
     4321 ttgtttgctg tcaatttcaa caaagtggtg agctttcaat cttattgggt aatgaaccat
     4381 cgtggtgcat accctccttt tatagggcag gggaacgcga cagctgatta aaggagcaaa
     4441 tgacgaatct atcataatct cttatttgac agttggttag cttgtacatc aacgccaaaa
     4501 taaaactggc aagcatcttg caatctggtt gtaagtaatg gcggcacttg ggccgattct
     4561 taaaaaccgg aggcaacatg aacttcagac gcctgaaata cttcgtaaaa attgtagata
     4621 ttggtagcct gacccaggct gctgaagtat tgcatatcgc acaaccagcg ctcagccagc
     4681 aggttgccac actggaaggt gagttaaatc aacaactttt gatccgtaca aagcggggcg
     4741 ttacgccaac agacgccgga aaaattctct atacccatgc gcgggccatt ttacgtcagt
     4801 gtgaacaggc ccaactggcg gtgcataacg ttggtcaggc attatcgggg caagtctcga
     4861 ttggctttgc accaggaacc gctgcgtcat ccatcaccat gcccttatta caggcggttc
     4921 gcgctgaatt tccggagatc gttatctatc ccgagcattg ctgaagtgca acgtgcgctt
     4981 tatgacgcca ccaagctggt gagcggcagg cccggtgagg aagtcaaaca gcggctacgt
     5041 actggtactg tggtaaccac agatgacagg aactgggaat tacgttactc agcttctgca
     5101 cttcgtttta acttaagccg ggccgtagca attgatatgg aaagtgcaac cattgccgcg
     5161 caaggatatc gtttccgcgt gccatacggg acactactgt gtgtttcaga taaaccgttg
     5221 catggcgaga ttaaacttcc cggccaggct aaccgttttt atgaaggcgc tatttccgaa
     5281 catctgcaaa ttggcattcg ggcgatcgat ttgctgcgcg cagaaggcga ccgactgcat
     5341 tcgcgtaaat tacgaacctt taatgagccg ccgttccgat aataagttct gaaccttctt
     5401 atcagaaata gatgtgaagg agtaagtaag acacctggca aatagcctgc aataatcgtg
     5461 ggctgtttgc ttccttgggc ggatacgtgt tttattatcg tcttaatgat ttccacatat
     5521 taaaagcaag tatgctttca aaacacaatt ataaaaaatc ccgccaacaa tataagtttt
     5581 tataaaatta aatataagat tatggcttta gaatattttt atttctaata gacgagattt
     5641 ttcctgttat gatataatat gccgaattaa cacatgttaa cgatttacca gtaatgtaaa
     5701 taaattttcg aggagatcat tccagtggga cgtaaatggg ccaatattgt tgctaaaaaa
     5761 acggctaaag acggtgcaac gtctaaaatt tatgcaaaat tcggtgtaga aatctatgct
     5821 gctgctaaac aaggtgaacc cgatccagaa ttaaacacat ctttaaaatt cgttattgaa
     5881 cgtgcaaagc aggcacaagt tccaaagcac gttattgata aagcaattga taaagccaaa
     5941 ggcggcggag atgaaacgtt cgtgcaggga cgttatgaag gctttggtcc taatggctca
     6001 atgattatcg ccgagacatt gacttcaaat gttaaccgta cgattgctaa cgttcgcaca
     6061 attttcaata aaaaaggcgg caatatcgga gcggcaggtt ctgtcagcta tatgtttgac
     6121 aatactggtg tgattgtatt taaaggatca gaccctgacc atatttttga aattttactt
     6181 gaagctgaag ttgatgttcg tgatgtgact gaagaagaag gtaacattgt tatttatacc
     6241 gaacctactg accttcataa aggaatcgcg gctctaaaag cagctggaat cactgagttc
     6301 tcaacaacag aattagaaat gattgctcaa tctgaagttg agctttcccc agaagattta
     6361 gaaatctttg aagggcttgt tgatgccctt gaagatgacg acgatgtaca aaaagtttat
     6421 cataacgtcg caaatctcta attatatatt aaagaaatct gtctttatgg cagatttctt
     6481 taatctcata taatccttat aaaaaatata atgttcaact cgtcatattg cttataaaaa
     6541 atcctcgttc ccagagaaat aatatttatt ataattccag ttcttctttt tctgattaca
     6601 gaaggcaaag tgacaattac gcatagtttc ccgataaaga cgcgacagct acatcccgca
     6661 taaggcattt ttctctttat ccttgtacgg tacttcatgg aacagagttt ttgaccttgc
     6721 gaatcgtgat gtctgttggg gaggggcaat ttgctcactg aagcgtgaga ctcgattaag
     6781 cgcacgaaac acagaaatca aaaaacccgg gcactttttt acaaggtaac cgggtaaaaa
     6841 taatttttat tttttaactg ttttgagact catagagatg tctcaaaact aaaatttggc
     6901 tcctctgact ggacttgaac cagtgacata cggattaaca gtccgccgtt ctaccgactg
     6961 aactacagag gaatcgtgag aacgaggcga atattagcga tgcccaccca caatgtcaaa
     7021 gcctgttatt taaatttgaa atcgtttgct gaaataatct gcattttgtc gtttattccg
     7081 acacaactgg ctttttttca cacttttgcg gttcggatcg agggtatttc cacagccaac
     7141 gtccagtaac cattcgccag taaaacagca cggcccgcac agcccagtcg gcaaacattc
     7201 ccatccagac accaaccaca ccccagccaa gcatgatgcc cagcacataa ccgacgacaa
     7261 cccggcaacc ccacatgctc aacatagaaa cccacatggc gtaacgggca tcgcgagcac
     7321 ctttaaatcc agcgggtagc ccccatgagg cggaccaaat aggcataaat aaagcattta
     7381 gccaaatcag aatcacaacg acatgtttaa cctgtggatc ctgggtgtaa aacgatgcca
     7441 taaccccggc aaagggagcc gttagccagg cgatggccgt taatccaaga gtggaaagcc
     7501 agaacacatg ccgcaactga atctctgctt gcgctatctg ccctaccccc aaccttcggc
     7561 ctgtaatgat cgtagaagca gagccgagcg cacttccggg taagttgata agagccgcaa
     7621 ttgaaaacgc gataaaattt ccggcaataa cactggtccc catcccggca acgaacattt
     7681 gggttaataa ccgaccactg gtaaataaca ctgattcgac actcgcggga ataccaatcc
     7741 ccatgacttc ccagataatg ctaaaattca gcggtttaaa atagctcttt aacgaaatcc
     7801 ttagcgcagg attaaaacca atcgccagca cccacaaaat tgcaactgcg ccaatataac
     7861 gagaaatggt taaacccagc cctgccccga caaatcccag tcccggccag gagaaaaggc
     7921 cgtaaatcaa tatgccgcta ataataatat taagaatatt caggctaccg ttaatcaata
     7981 gcggtatttt cgtattccct gcaccacgaa gtgccccgct accaataaga gtgatggcag
     8041 ctgctggata actgagtacc gtcagctcca gataagtcaa cgccagtact ttaacttctg
     8101 tcgtggcatc acccgcgacg aaatcaataa tttgttcgcc aaaatgatga ataagcgttg
     8161 ccaacagtac ggcaaacaac gtcatgatca ccaatgactg ccgcgtcgcc accctcgctc
     8221 gtcgtcgatc ccgcttaccg agactaaatg ccacaacgac agtagtacca agatcgatag
     8281 cagcaaaaaa agccataatg accatattga agctgtccgc caatcccacg ccggccatcg
     8341 catcttttcc cagccagctg accagaaaag tgctcagaac ccccatcaac aggacacagg
     8401 cattctccat gaagatagga acagcaagcg gggttatctc gcgccagaac aacactttgt
     8461 agctcttgcg tttagcgtgc cagcgagtgc cgtgaacaac ctggcgtaaa gcagaggaga
     8521 tattcaaagc cgaccttaat tgcagaaagt gaaaccacat ttcaaataat gagggagaat
     8581 cagcaaagct gcaaagattt tcgccaacaa attgtctgca aatgcaacaa actgttgata
     8641 gaaacggcaa acagttgggg aatttaaaaa tcgggtttga caaaagattt ttcgccgtta
     8701 agatgtgcct caacagcgat tcctctgtag ttcagtcggt agaacggcgg actgttaatc
     8761 cgtatgtcac tggttcgagt ccagtcagag gagccaaatt ccgattctta cgctatctta
     8821 ttagtcctta tttgatattg attcaataag ttagcgtgaa aatctttcca gatgtgtatt
     8881 cagttttccc tccgcatccg gttaatttgg tggtcagatt tgggggcagg ttggttcgat
     8941 tctggagtga cccccaaatg tctttaaacg attcaaaaat ccgtaactta aaaccctctt
     9001 caaaacccgt aaaactgtct gattcgcacg gtctgtatct gctggtcaat cagggcggtt
     9061 cgcgtatctg gtatctcaag tatcgcttca gtggtaaaga gtcccgcgtc agtctgggcg
     9121 catatcctct ggtttcactt gctgaagcaa gacaacgacg tgacgatatc cgtaagctac
     9181 tgacgcagaa tattaacccc gcccaccagc gcatgtctga taaagccgca gcgtcaccgg
     9241 aaaagtattt taaagccgtg gcgcttgcat ggcacaaaac caacaaaaaa tggtcgcctg
     9301 attatgccgc gcgcatcctc gccagcatgg aaaatcatat ctttccggcg attggtcatc
     9361 tgcctgtcac tacgctgaaa acgcagaatt tcactgcgtt actgcgggtt attgaggata
     9421 aaggttttct ggaagtcgcg tcccgaaccc ggcagcaact ctgcaacatc atgcgttacg
     9481 ccgtacagca gggattcacc gaaaacaacc tggcgcagca tctggaaggc gtcaccgcgc
     9541 cgccggttaa gaatcactat cccgccctgc cgctggagcg gttgcccgaa ttgtttgagc
     9601 gcattggcga ctatcagcag ggacggcagc ttacgaggct ggcggtggtg ttgactctcc
     9661 acctgtttat ccgttccagc gagctgcgct ttgcccgctg gggtgagata gatttcagaa
     9721 acaaaatctg gaccatcccc gccacaaggg aagcgattga gaaagtccgt ttctctggcc
     9781 gtggcgcaaa aatgcgtact ccatatatag tgccgctttc tcgtcaggct atcgccattc
     9841 tgaagcagat aaaggaaatc tccggccatc tggaactggt gttccccggc gaccataacc
     9901 cgtataagcc gatgagcgaa aacactgtca accgggcgtt gcgtcttatg ggctatgata
     9961 ccaaaactga tgtctgtggg catggattcc ggacaatggc gtgtagcgca ttagtggaaa
    10021 ctgaactctg gtcacgggat accgtggagc ggcagatgag tcatcaggag cgcaacagcg
    10081 ttcgggcagc gtatatccac cgggctgaac atctcgacgc ccgtacagcc atgatgcagt
    10141 ggtggtcgga ttatctggat gtaagtcgcg aggggtacgt tgcgccgtat atttatgcgc
    10201 ggcggcataa ggcggcctga gacagtggct atataatttg ttaaaaatga aaaagcagat
    10261 cctgattgaa tccggatctg ctttttacat taggcaatcg ctgccagaca atctgcacca
    10321 gaaacgccat aactgcacca gttcagtacc accgcttacc tgcgccagtc tgccgtcgcc
    10381 tgtctgtgtc aggactgcgc cagatcttat cgaataaaca tcaaactgcg ccagtgttac
    10441 ggccctttca gccctttggc cttttccggt ctggcgctgt tacccttagc cgccccttac
    10501 cctttccggc ctttttcctt tgctggcgca atcgccagaa aaatcgtcaa gctgcaccag
    10561 aagcctgcca ataggtcata ccgttcagaa gcatcgttat tgagaggaaa ccgtacaaaa
    10621 aatttctacg tagaaatttt ttcggtggat aacgatgaat aaagagtatt ttataatgtg
    10681 atggctatca aaaatcgacc atccaaaaat aattcattga tttataatga ataaccatga
    10741 atttaatagt cgatgattat tgtttttatc agtattgata ggaaagtata caaaaaattt
    10801 tggccccaaa attttttgta cgaaaaccga tgaataacgc gaaattaata ggaaaccatc
    10861 caaaattatt tggggcaaat aattttgccc gaaaaccgat ggataactcg atcttttcaa
    10921 agcaacggct atcaatcagt atcctttctt ccgtaaacct gcgccacata ccgcccgcgc
    10981 ccgtcgccgt gtcccaaatc catcgccgtc attgccagcg cttctttctc actgaatccc
    11041 tgcgccagat aatgacgaat ggcatcctgc gcccacgcat agcgcaacga gtgcggggaa
    11101 tatgtgccag ttaacccgag acgtgacgcc tggctgtgcc agtatttcat cgcgcttttc
    11161 aggtcgggtt tatctatcag cctgccgtgg cgatcttctg ccacaaccaa cgcattatcc
    11221 agcgcttttc tgatagcgac agcatccaga ataacggttt ctcgcggacg cccgcctttg
    11281 gtgccgaaca ccacggttaa acggggctca ccccgctcca gcgattgctt ccacgttctt
    11341 aatgactgcg cactttgcac cacctcctgc gaacgtaatc ccatcagcct tgagagttcc
    11401 agcgccgctg ccatccccgg atcttttgcg cgggcggttt ccagcacata gcggtaatgc
    11461 tccggcgtga tggcccgctt cgtaccgtta cgggatgcgc cagacaggcc cagcgagcgg
    11521 ttattcagtc gctcactctg cgccagcctg tcacgcccgg cctgtttcag aatgcagcgc
    11581 accgccgcca tctcattttg cagggaacgc ttcgtgatac cctgcgccag tcgttcacga
    11641 acatatccct caatataccg cgccttaagc tgctccaccc ggcggatctg cacattctgt
    11701 gccagaacca gacgctcaca aaaccgctgc accagcttaa tacggtcgtg aacggtctta
    11761 tggctgccgc ccgcctgttg tgccaatcgc ttcatttcct gctccagaat ccccatacat
    11821 tccccctttc atgattgccg caaactcatc cccaaaaact caacactgat acagcgttcc
    11881 ccggcgcaca ggccgatagc ccctgagttt tgtcggggct gcgaacgata cctgagcgcc
    11941 ggggcggcag ccgttgaggt gtggtggggc ttcggttgtg ctctcagagc agccgcccgc
    12001 ttgcgcgggt catcccccgg atcgtccaga tccgcctcgg tatcggttca tattctcctt
    12061 atgggtaaat ggcctaaaaa ggcggtgtca gaatccagcc atgagctggc gcagttgagt
    12121 tttctggtgc aggtcgaaag ggcttgcgtg cgtcactttc ccgctttgcg cggttaaagt
    12181 gacgcacgga tctggtgcag catctgagat ttatcgtctg gtgcagtttt acggtcacgc
    12241 ggcagcaaag cctgacggca atgcaggccg cagcccgtaa ggtaaaaggc gctgagggtg
    12301 tcagcagatg caggttagcc gggagaattc cggcgcggtg cagattcaga ccagcaggct
    12361 ggcgaagtaa aaggatgtat cgtattaaaa ctctggcctc cgtcgcgatg ttctgcgacc
    12421 cggtgtttgc agaggtctgc accggtaaac ggatcgaccg tcctgataac aggcgcttct
    12481 ttcaggttgt gaagcgttgg gcgctgtcta cgacagcata gagcaagggg gagactacgc
    12541 ctgtgccgct ggcgcggaaa ggagaaactg tttttacctg caaaaggatt ttttaatcac
    12601 ttgttgttat aaaaaatgag ggcgctgtat gtgacagcgc cctcgttcag gttacataaa
    12661 aaatatcagc tcagaatagc ttacagtcga gccagcacct cattcacgac agcatcgggt
    12721 atacgttcca gccgcttacc gctccgagct cccatatcaa tggttctggg ctggtcgcag
    12781 cgaataacgc ccgttgtttt tgttcccgcc ccgtccagtg aaacggtaaa accggcttcg
    12841 cgggcaaagt ttccgccgct ggtgacaggg acaacaacag gcaaccgggt gaacgtgtta
    12901 aacgatgcct tcgatacgat gagtaccgga cgttttccac tttgttcatg gccagtggtg
    12961 ggatccagtg aaacgagcca gatttctcct ctgtccatct acaggatctc cttgcccacc
    13021 gcaggcgcat caatccattc ccgaccctct tcgcttattt cagcgtgcgg atcgcactgc
    13081 gccaacagtt cttcgagcga atagcgggga cgtttttgag gttcaataat caggcagcca
    13141 ttatcaatgg tcatgcccac ttcgctgtcc gtcgacagcc ctagcgtttt cagcactgcg
    13201 gggggaaccg ccagcatgac ggatccgcca acctttttca ggcgagtggt atacatagag
    13261 cacctccgga tattatattt taatataaca tccatgatag acgatgcaca actattcatc
    13321 agattcattg tggtttcctt agtgtaataa agcgtgaaga aagccttaat ccatcggaag
    13381 acagatccgg cagttttcaa aggcgacatt gccggtaaaa gggcaaccgt cctgatacat
    13441 caggcgcttc tttcaggtca tgaagcattt cgtacaggcg ggagcttagt gcgtaacaaa
    13501 gaaggccgat aataggaaac tgcttttgca gttcaaagga ttatgacggt tcggataaac
    13561 aaaatgcggc gcagcttccg tatccgggct gcgccgcagg aacatcagcg gtagcgcgaa
    13621 ggccagattt gctcaggctt gatgcctaac gcttcggcaa ttaacctttc gcctttgggc
    13681 cagcggcggg taagcgcatt agccagcgtg gaggacgcca gacctgcctg acgcgaaagc
    13741 gctgacagcg aagtgcctcg ttttttcagc ccggcgataa tgtcggcagg atgccagtcc
    13801 tgcagcatca tgcagccacc tcgtcgataa acttcacgcc atcggcggtg cggagccagc
    13861 gcggggcttt catttcggcg gcaaaatacc agaccagttc acataataag cctgttatcg
    13921 tctgctccac ttttggctgc atcccctgcc cgataagaag ttgcgccagc gtcagtgagt
    13981 aatcacaaag taattctgcg tcggggacaa atcggggcat atcggcaggt agagcatcaa
    14041 cggtcaggct gtcagtcaga tgagacggga tggggtcgtt cagcgtgggt tgcagcaaag
    14101 tgagacaggc ggcaagtcgc ccgcaaagcg ccagccgcag caccggatct tcgctttcga
    14161 tcagggtttc ggcgacgttt tcgcagtggt ctgccagtac ggtaaaatct gtggcagcac
    14221 tgaagggaac agcaagtaac gggtgtgtct gagtggggat cgtagccata aaggcagcct
    14281 ccagtatgtg gtttgaaaac caccaccaga ggttccaatc tcatgggtgg tggactgaac
    14341 gaggttggaa ctaccggcca tactggaacc ggcgcacctt acggtgcccc cgcccagccc
    14401 accataattt tggtgtagct aagcgcgcga cataaaaaaa gacgcagacg cgtcacgtgt
    14461 cgccagtatg gttatatttc ggggttccaa tcccggcacc tgattttgca ggtgcgggta
    14521 aaagataccg ctttcggttg aaaacggcaa ggggaagtcg tggaatagag atcgcagatt
    14581 gttataaata tacacaatag ttaatgttaa caaagacggc acatatatta actttactct
    14641 caatctgctg aatagtttat cgcatgtaat tctcgggtca tatagtggat accctccttc
    14701 ttttctttca tcccctcttg cctcaccata aaagcaaacg aaaaaattgc tgaacgagtt
    14761 atctgaagta aatgctcaaa atggtgaata aaatcacttt ctttgataaa aagaatatct
    14821 tctattagtt gataagattc gtaaatgtcc tcaggtttat cacttttatg tacaactaaa
    14881 aacttatgct ctaaaccatt gcgccattct ttatagaaag ccaactcccc acccttatct
    14941 ttattcaggt cggtagcaat actgtacagc gccagaagcc ctggtgattt taccttttca
    15001 aataattgtc ttctgttatc aacatctaac tgccagaaat tctgaaaata gatattcttc
    15061 tgaggtgtac ctttttttgt tgttgggtaa agattataaa gttcacatat ggctaccgca
    15121 attttatcaa gaataccaaa gcaaagacga aatgccgtcc tgattttttc aatttctatt
    15181 cctaaaactt catcattata caattccaga aaatggcttt cgtacttaat gtaatccgta
    15241 ttttgtggat gaagataatc aaaataaaga tggcgtgcga gtgaaaactc cgatttaagg
    15301 cgatttaata ccatctccat gggaatgata aaatcacctg ttacaccgcc tcgcgaagca
    15361 atcacaagat catctgtagc actccctaca catgggcaat acaggccatg ttctgataaa
    15421 gtcagatgat ttctaagaca aaactgtcga tatgggctta acacttcgaa ctcttgttgt
    15481 gtaagtgctt catcgtgttc gtcagtttca tcatcaacag catattctgc gattttctcg
    15541 ttagtctgcg ctatgcgttc taaatagaac gattcccatt gtggtgggat attttttgat
    15601 acagacgctt taatatatcc tgctctcact tcttttagta acttgattga aaatgaagat
    15661 gaaataagat tgaggaccat aagtgacatc gaacggttga cccaagcctg tggtatatct
    15721 aatcccttaa gattggtaag atcataatat ctcaatgact cactgagccg aaattgactt
    15781 cttaatgaat tagccaaatt aacagatagt tcagcctgaa attcctcctg ctcctcagca
    15841 gatagcttaa aagaacgcca gtagatattt tttaaggaaa ccaattcttc tatcgatttg
    15901 aatgtatggt cattggggtt agtaatgctc accagattag atagcgcatt accatagtta
    15961 taatagtaaa ggcactctcc taacactgat ataaatgctt ctttgttatc ttccatcagg
    16021 ttgcaaccaa gctctgacgc atttttatta ttttgcatct gcccgatgtc aacaagagca
    16081 cccgcaatgt tcgccaatac tataaagtag ttatcgttcc tgattgttat tctttccagt
    16141 ttgttcagga acgcaactga ctgttgataa gattcgtcaa atcttccaat atcaaccagt
    16201 tggctaatga aattactttc atcgacaagt tcttttgtta tcaaaataaa ttttccttat
    16261 acagaggatt tcggtgcttc aacaggaggt atgccctcac aggagacatc tataactatc
    16321 gctgataagc ggtctctttt atcaaccggc acagtttcat taagcatatt ttccattgca
    16381 ctggcgtttt caggttgaga tgtatttttc gccaaatgag gcagggaatt cggtcgagca
    16441 gaaccaaacc aaaaaaccag atagataccg tgaccagcag catcggggtt tttagtataa
    16501 agcttgtcca attgcccatt aagtgcagtc cagacatcac gatgataatc acgctttatc
    16561 tcgataggta tttttatctg cggtagtgaa acaatgatat ctgccctttt atcagaaacc
    16621 atgtgtcctt ctggttcaca gcttattttt tatggattaa gccggacacg gagtaaatcc
    16681 agaaaaatat tacggcaact ctcttcaggt ttaggcgtta gggtacggcc atagctgtct
    16741 tcattccaga attgcttata aatatcggta ttttcattgg caattcggtt agaaatatcc
    16801 cgtatatgat caagcaataa tgagtaaaga tccattatgt tggcaggcgt tttattcatc
    16861 aatgtactta cagtctgcct ccagtcggca tgatggaact gtgattctct ataacgaatt
    16921 ttctgattgc tttgggcatg tgagagatga tcccgataac tttcacattc aggcagcagt
    16981 aataagcgct caagtacttc actagcatcg t
//
LOCUS       DAJTMC010000084        16749 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.084, whole genome shotgun sequence.
ACCESSION   DAJTMC010000084 DAJTMC010000000
VERSION     DAJTMC010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16749
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.084"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            <1..2017
                     /gene="fhuE"
                     /locus_tag="OO130_002451"
     CDS             <1..2017
                     /gene="fhuE"
                     /locus_tag="OO130_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HCR5365820.1"
                     /translation="DYSVTSTSAGTKMQMTQRDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLEISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAIDMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTTLSAGYEYQRIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQATLNATHSEVE
                     FDSKMMYVDAYVNKADGMLVGPYSNYGPGFDYVGGTGWNSGKRKVDALDLFADGSYEL
                     FGRQHNLMFGGSYSKQNNRYFSSWANIFPDEIGSFYNFNGNFPQTDWSPQSLAQDDTT
                     HMKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLAPITGNNYELGLKSGWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNMEGSIVYGAPRNFSITGTYQF"
     gene            complement(2077..3510)
                     /gene="ptsG"
                     /locus_tag="OO130_002452"
     CDS             complement(2077..3510)
                     /gene="ptsG"
                     /locus_tag="OO130_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HCR5365821.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMHDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(3805..4602)
                     /locus_tag="OO130_002453"
     CDS             complement(3805..4602)
                     /locus_tag="OO130_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR5365822.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(4613..5617)
                     /gene="holB"
                     /locus_tag="OO130_002454"
     CDS             complement(4613..5617)
                     /gene="holB"
                     /locus_tag="OO130_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HCR5365823.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPSAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(5614..6255)
                     /locus_tag="OO130_002455"
     CDS             complement(5614..6255)
                     /locus_tag="OO130_002455"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HCR5365824.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(6245..7267)
                     /gene="yceG"
                     /locus_tag="OO130_002456"
     CDS             complement(6245..7267)
                     /gene="yceG"
                     /locus_tag="OO130_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HCR5365825.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(7270..8079)
                     /gene="pabC"
                     /locus_tag="OO130_002457"
     CDS             complement(7270..8079)
                     /gene="pabC"
                     /locus_tag="OO130_002457"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HCR5365826.1"
                     /translation="MFLINGHKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMIFCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTVYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(8199..9440)
                     /gene="fabF"
                     /locus_tag="OO130_002458"
     CDS             complement(8199..9440)
                     /gene="fabF"
                     /locus_tag="OO130_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HCR5365827.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKAIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNLDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(9528..9764)
                     /gene="acpP"
                     /locus_tag="OO130_002459"
     CDS             complement(9528..9764)
                     /gene="acpP"
                     /locus_tag="OO130_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR5365828.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(9975..10709)
                     /gene="fabG"
                     /locus_tag="OO130_002460"
     CDS             complement(9975..10709)
                     /gene="fabG"
                     /locus_tag="OO130_002460"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HCR5365829.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(10722..11651)
                     /gene="fabD"
                     /locus_tag="OO130_002461"
     CDS             complement(10722..11651)
                     /gene="fabD"
                     /locus_tag="OO130_002461"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HCR5365830.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVPVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(11667..12620)
                     /gene="fabH"
                     /locus_tag="OO130_002462"
     CDS             complement(11667..12620)
                     /gene="fabH"
                     /locus_tag="OO130_002462"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HCR5365831.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVANQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(12688..13758)
                     /gene="plsX"
                     /locus_tag="OO130_002463"
     CDS             complement(12688..13758)
                     /gene="plsX"
                     /locus_tag="OO130_002463"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HCR5365832.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRMALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLRPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(13839..14012)
                     /gene="rpmF"
                     /locus_tag="OO130_002464"
     CDS             complement(13839..14012)
                     /gene="rpmF"
                     /locus_tag="OO130_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HCR5365833.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(14064..14585)
                     /gene="yceD"
                     /locus_tag="OO130_002465"
     CDS             complement(14064..14585)
                     /gene="yceD"
                     /locus_tag="OO130_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HCR5365834.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            14784..15368
                     /gene="yceF"
                     /locus_tag="OO130_002466"
     CDS             14784..15368
                     /gene="yceF"
                     /locus_tag="OO130_002466"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HCR5365835.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLCEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(15480..16439)
                     /gene="rluC"
                     /locus_tag="OO130_002467"
     CDS             complement(15480..16439)
                     /gene="rluC"
                     /locus_tag="OO130_002467"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HCR5365836.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRSQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
BASE COUNT         4159 a         4546 c         4047 g         3997 t
ORIGIN      
        1 tgattacagc gtaacgtcta cctctgcggg taccaaaatg cagatgactc aacgtgatat
       61 tcctcagtcg gtcactattg ttagccagca gcggatggaa gatcagcagt tacaaacgct
      121 gggcgaagtg atggaaaaca cgctggagat cagcaaaagt caggcggatt ccgatcgtgc
      181 tctttattat tcccgcggat tccagatcga taactatatg gttgatggta tccccaccta
      241 ttttgaatcg cgctggaatc tgggcgatgc actttctgat atggcactgt ttgaacgcgt
      301 agaagtagtg cgtggcgcga caggactcat gaccgggacg ggtaatccat ctgcggcaat
      361 tgatatggtt cgaaaacacg cgaccagtcg tgaatttaaa ggcgatgtct cggcggaata
      421 cggtagctgg aacaaagaac ggtatgtggc ggatttacaa agcccactca ccgaagacgg
      481 taaaatccgc gcgcgaattg tcggcggcta ccagaataac gactcatggc tggaccgcta
      541 caacagtgaa aagaccttct tctccggcat tgtcgatgct gatttaggcg atcttacgac
      601 gctttcagcc ggttacgaat atcagcgcat tgatgttaat agccctacct ggggcggttt
      661 accgcgctgg aatactgatg gcagcagcaa cagttacgat cgtgcacgca gtaccgcacc
      721 tgactgggcg tacaacgata aagagatcaa caaggtcttt atgaccctga agcagcggtt
      781 tgctgatacc tggcaagcga cactgaatgc cacccactct gaagtcgaat ttgacagcaa
      841 aatgatgtat gtcgatgcct atgtaaacaa agcggatggt atgctggttg ggccatacag
      901 taattatgga cctggctttg attatgtcgg cggcaccggt tggaacagtg gcaaacgtaa
      961 agttgatgcg ctggatttgt tcgctgacgg tagttatgaa ttgtttggtc gtcagcacaa
     1021 tctaatgttt ggtggcagtt acagcaaaca aaacaatcgt tacttcagtt catgggccaa
     1081 catcttcccg gatgaaattg gcagtttcta caactttaat ggcaatttcc cacaaaccga
     1141 ctggtcacca cagagcctgg cacaggacga taccacacat atgaaatcgt tatatgctgc
     1201 cactcgagtc acccttgccg atccgctgca tctgatcctc ggcgcacgtt ataccaactg
     1261 gcgggttgat acgctgactt acagcatgga gaaaaaccac accacgcctt acgctggtct
     1321 ggtgtttgac atcaatgaca actggtcgac ctacgccagc tatacctcta ttttccagcc
     1381 gcaaaatgat cgtgacagtt caggcaaata tctggctcca atcaccggta acaactacga
     1441 gctgggtctg aaatcgggct ggatgaatag ccgtctgacc accacgttag ccatcttccg
     1501 tattgagcag gataatgtcg ctcagtccac cggtacacct atccccggca gcaacggcga
     1561 aaccgcctat aaagcggtgg atgggacagt cagtaaaggg gtggaatttg aactcaacgg
     1621 cgcaattacc gacaactggc agctgacatt tggcgcaacg cgctatattg cagaggataa
     1681 cgaaggaaac gccgttaatc ctaatctgcc acgcaccacg gttaaaatgt tcaccagcta
     1741 tcggttgcct gtcatgccag agttgacagt cggcggtggt gtgaactggc aaaatcgcgt
     1801 gtataccgac accgtgacac cgtatggcac cttccgcgcc gagcaaggta gctacgcgct
     1861 ggtggatctc ttcacccgct accaggtgac gaaaaacttc tcgttacagg ggaacgtcaa
     1921 taacctgttc gacaaaacct acgataccaa catggaaggt tctatcgtct acggcgcacc
     1981 gcgtaatttc agcattaccg gcacgtatca attctgaata acacctgtaa aaaaggcagc
     2041 catctggctg ccttagtctc cccaacgtct tacggattag tggttacgga tgtactcatc
     2101 catctcggtt ttcaggttat cggatttagt accgaaaatc gcctgaacac cagaacccgc
     2161 aaccactacg cccgctgcgc ccagtttctt caggccagcc tgatccactt tagacacatc
     2221 agcaacgctg acgcgcagac gggtaataca tgcgtcgagg ttagtaatgt tttctttacc
     2281 accaaatgca gcaaccagag ccggtgccat ttcgctggta cctgttgctt ttgcatcttc
     2341 agtcgcgtct tcacgacccg gcgttttcag atccagtgct ttaatcagca cgcggaagat
     2401 ggtgtagtaa acaatcgcat aaccgatacc gacgatcggg aacagccaca gtttgctgct
     2461 gttaccagac agaacgataa agtcgatcag accgtgcgag aacgacgtac cgtcatgcat
     2521 ccccagaaga atacagattg ggaatgccag gcctgccaga atcgcgtgga tgatgtacag
     2581 gatcggcgca acgaacatga aggagaactc gatcggctcg gtgataccgg tcaggaacga
     2641 ggtcagcgcc gcggagatca taataccgcc cactttcgcg cggttttctg gtttagcaga
     2701 gtgccagata gcaattgcgg cagctggcag accgtacatt ttgaacagga agccaccaga
     2761 cagtttaccc gcagtcgggt cacccgccat ataacgcgga atgtcgccgt ggaaaacctg
     2821 acctgctgcg ttggtgtatt caccaatctg catctggaaa ggtacgttcc agatgtggtg
     2881 cagaccaaac ggtaccaggc aacgttcgat gaaaccgtaa atgccaaacg caactaccgg
     2941 gttctggtaa gcagcccact gagagaaggt ctggattgca gaaccaatcg gcggccaaat
     3001 gaaggacagc acaacgccag taaagatggc agccaggcca gaaatgatcg gcacaaagcg
     3061 tttaccggca aagaagccaa gatactcagg cagcttaata cggtagaaac ggttaaacat
     3121 gtacgctgcg atcgcaccgg agataatccc tccgagtacg ccagtatccg ctaggtgttt
     3181 agaggcgatt tcttcagcag gtaaatgcag taccagtggc gcaaccacgg ccatggtttt
     3241 aaccatgatg ccataggcaa caactgcggc cagcgcggat acgccatcgt tattggtaaa
     3301 gccgagggcg acaccgatcg caaaaatcag tggcatgttt gcaaagacgg aaccgcctgc
     3361 ttctgccata acatgcgata caacggcggg cagccagctg aaattcgcgg aaccgacgcc
     3421 cagcagaata cctgcgatag gtagtacgga taccggcagc atcagcgatt taccgacctt
     3481 ttgcaggtta gcaaatgcat tcttaaacat aattgagagt gctcctgagt atgggtgctt
     3541 tttttacgtt ctcacgcatg gcaagggggg agagcctcgc cgtgtacagg gcatctaagc
     3601 gccctttatt tattacacag agtaaaataa ttcagtgcca atttgtttga cggctatcac
     3661 gtttcaactt cagatcattg gagaagttgc acattttgac aaaacatatg tcacaatatt
     3721 tcaaacgcgg ttattcaccg cccaattttc ggcggtgaac tttactcgtt ttagccatta
     3781 attacgagct ttaaaaaaac tcattcaacg gatggattga aggcgggaag cgtcgatgtg
     3841 aaacagacgg gcgaagttat cggtggttac ctgcgccagt tcttcaacgg caacaccttt
     3901 caacacagcc atgtattctg caacgtcacg aaccatcgca ggttgattct cttttcctcg
     3961 atgcggtacc ggcgcaaggt aaggtgagtc agtttccacc agtaatcgat ccagggggac
     4021 ataacgcgca gcatcgcgca gttgctccgc attacggaag gtcacaatgc cggaaaagga
     4081 gatgtaaaat ccgagatcca gtaatttacc cgccgtttct ctgtcctctg taaaacagtg
     4141 tagtacgcca ccgcaatccg tcactttttc ttcgcgcaga attgccagcg tatcggcacg
     4201 ggcgtcacgg gtatggacga taaccggctt gttcagttca cgaccaatct ggatatgatg
     4261 gatgaaggac tcttgctgac gtactttagt ttccggtgtg taataataat ccagcccggt
     4321 ttcacccagc gctacaacac cctcttctgc cgccagacgg cgtaaatctt ctacatcgta
     4381 gggatcattc tggttgagtg gatgcacgcc acaagaaaat acgacgttgt cacgttcgcc
     4441 taccagatcc cgcatatgta aataacccgg taatgttgtg gcgactgcca gacaaaattt
     4501 cacatcgcgt gcggcggctt tcgccagaac gtcatccacg tccttatgca aagattcata
     4561 atccagacca tcgagatggc agtgtgagtc gactaaaaac atgatgtctc tcttaaagat
     4621 gaggaaccgg tagcacaacg cccggttgca ggtaatgctc aatacgcagt aaaagatcgg
     4681 tgatgagaag ctcgcggttg atgcctgtga cagacattaa ctgttcacga atgtggcaaa
     4741 catcccccag tatagcctgc aggcgcgagg gagaaagatg gtttgccagt tcggcgacca
     4801 ggcccggcac atcaacattg gtcacctgtg cagcgccatg atggcgtttt agcgcatcca
     4861 tcagcaacgt tgccagccag tgtaaacgcg ccggagcttg ttcatgatta agggccgcca
     4921 gcagcgaata ccagtcgccc gatggtacgc tatatgccaa cgcctgacac aatgtttcac
     4981 gagcctgcca gttatctccc tgaaacaacg ccagtgccgc gctaggcgaa ccggcactta
     5041 agcgcaatgc ggcaagtaat gcatcctgtg acattgtcac ttcgcgtgaa agccaggtca
     5101 cggcgtactg ttccggcggc ggcgcaaggt aatgtaaccg acaacgacta cgtaatgttg
     5161 ccagtaaacg ttcaggctcg cgggtagcca ggaaaaacca ggtttctgct ggtggctctt
     5221 caagcgtttt cagcaatgcg ttagccgcgg cgtcggtcag taaggcagca tcggttaccc
     5281 agacaacttt cgcaccacct aggcgtgcgt gctcattcag cttttcggtg acctcacgta
     5341 ccgcatcaat gcccagcgta tttttccctt tttcgggagc cagggtgtag taatcgggat
     5401 gcgtgccagc ctgcatcaac tgacatccac gacagtgacc gcaacttttg tggccctgcg
     5461 gttgttggca gagtaaataa cggctcaggg cgtagattaa agcatcatcg cccatgcccg
     5521 gtaacgcctg aatgagtagc gcatggtgac ctcttccggc ctgatagctg gctaccagtt
     5581 tttcgaaatc aggtcgtaac catggatacc atctcatgcg tccaactcct tcacccagtg
     5641 ggtcacggta gtgcggattg catccatcac ggcctccagc ggctgggtgg catcaatggt
     5701 atgaatactt ttatcttgtg cagccagttc cagatagcgg gcgcgggtgc gattaaagaa
     5761 atcgaaagac tcttgctcaa tacgatccag ctcgccgcgc gcacgcgcgc gttttaagcc
     5821 aacttccggg gtaacatcga gatagagcgt taagtcaggg cgaaaatccc cgagaacagc
     5881 atcacgcagt gttgccagca tatgttggtc aataccacgt cctccgccct gatacgcctg
     5941 agtggagaga tcgtggcgat cgccaatcac ccaggtgccg ttagccagag ctggtttgat
     6001 gaccgtttct accagttgaa cgcgcgcggc ataaaacatc agaacttcgg ctttatcggt
     6061 aatgacttca tcgcctaccg atttgatatc cagcaccagg cttcttaact tttcggcaag
     6121 ttgcgtaccg ccaggttccc gagtgaaaac catgtcgcgg atacccagtt gctcaagcgt
     6181 ctcaaccacc acattacgcg cggtagtttt gcctgcgcct tccagcccct caatgacgat
     6241 atacttactg cgcatttttt tccttaagca ctttcagata atcctgcaca gacttgttat
     6301 ggctggcaag attggtatta aacgtgtgac cacctttacc atcggccaca aaatagagat
     6361 acggcgtttt tgccggatgc gcagcagcct tcagcgaatc cgcccccggc gtagctatcg
     6421 cacctggcgg caaaccggta atggtatagg tgttatacgc tgtcggcgtt tccaggtctg
     6481 cacgagaaag tttgccatta taacgctctc ccatcccgta aatcacggtt gggtcggtct
     6541 gcaggcgcat accaatgcgt aaacggttga taaataccga ggcaacctga tcgcgttcac
     6601 tggcaacggc ggtttctttt tcgataattg atgccatcgt caccagctgg tttttatctt
     6661 tataaggcag gccgtccgca cgcccttccc aggcgctatc gaccgctttc accattttct
     6721 tgtgcgctcg cttgagtaac gcgacatcgg tggtattggc ggtatacatc caggtgtctg
     6781 gccagaacca accttcaatc cactccgggt tttccagttc aagtgcctgc gctacggtgg
     6841 cgtacttatc gtcactcagc gtgtgcttga tatacggagc ctcacgcaat tgcttgaggt
     6901 agtcgctcag acgcatccct tctaccagtc gcagcgggaa ctgtgcttct ttaccgcttt
     6961 ccagcaattt cagcatctcg cgcacggtca tctgcggtgt aaagcggtaa gtcccggctt
     7021 taaagtgaga aagatccggt tcgatacgca gcaaccattg aaaaacccgt gggcgattga
     7081 tgatcttatc ggcataaagc tgttcaccaa gcgccagacg tccggtccct ggcttcaggg
     7141 taaatatcgt ctcttctttg ataagcaatt tgctgtcggc aagatggcga accttccaga
     7201 cgcccacacc agcggcgata cccagtacca ccagcaataa caagataatc aataacactt
     7261 ttttcatgac taattcgggc gctcacaaag tggggctaaa tattcatata acgttgctga
     7321 cgaaaaggag acatcgccac aggcacgtac gggcatcact ggcattaacg cattacaaat
     7381 aaccatctca tctgcctgca acgcctcttc cagagaggct tgcacttcga caagctgata
     7441 agaggattgt gccagcaaac ggatacagaa ttgtcgcata atgccgttaa cacctgcctg
     7501 atccagtcgc ggcgtataaa ctacattgcc cttccgccag aacaaattag ccgcacagca
     7561 ttccgtaacc cacccttcgc tgtcaaggac cagcgcctca tcagcgtttg tctgctcaag
     7621 atgagagcgg atcaatactt gctcaagccg attaagatgt ttaatacctg caagatgggg
     7681 attgcgcccc agccgcaccg ggcttagcgc caacgtcatc ccttcgttac gcaaacggtc
     7741 gtaatgtgca ggataaaccg taacggagag aatccgcgtt gccggtccac tgttcaatgt
     7801 gctgtaccct cgcccgccac taccgcgact gatcacgact ttcagcacac cattttgctg
     7861 ttctgctgcc agcgttttca tctcttgttc aagctgaggc cagaagtcac agaaaatcat
     7921 caaccgctga caagcatcct gtagtcgctg gatatgcgcc gataacaaac tgactttacc
     7981 gtcgataact ctggcggtgg taaaacaacc atcaccaaac tgcgttgccc gatcgcttac
     8041 tgccagcgat tcctgcttat gaccgttaat taagaacata ccggctcctt atcagagaat
     8101 tgttagtgtg gcagcatgct cactacggaa caagtcggaa taaaagctaa gaaaaaaggc
     8161 ccgcaagcgg accttttata tgggtgggaa atgacaactt agatcttttt aaagatcaaa
     8221 gaaccattag tgccaccgaa gccgaaggag ttacacagcg tgtattccat tccgctgacc
     8281 tgacgtgctt catgcggtac gaaatccaga tcgcaacctt catccaggtt atccaggttg
     8341 atggttggcg gaacagcctg atcgcgcagc gccaggatgg agtagataga ctctactgcc
     8401 cccgccgcac ctaacaggtg accggtcata gatttcgtgg agcttaccaa cacacggctt
     8461 gcagcttcac cgaagatggc tttcaccgcc tgcgcttcag ctttatcgcc agccggcgta
     8521 gaagtaccgt gcgcgttaac gtagccaatc tgactcgctt caatgcctgc atcacgcaga
     8581 gcatttgcca ttgccagagc tgcgcctgcg ccattttctg gcggtgacgt catatgataa
     8641 gcatcgctgc tcataccaaa gccgacgagt tcagcgtaaa ttttcgcacc gcgttttttc
     8701 gcgtgttcgt actcttctag taccagcata ccggcaccat cgcccagtac gaagccatca
     8761 cgctctttat cccacgggcg gctcgccgct tgcgggttat cattgcgggt agataatgca
     8821 cgtgccgcgc caaaaccacc aacgcccagc ggcgtactgg ctttctctgc gccacctgca
     8881 accatcacgt cagcatcgcc atacgcgata atacgcgcag catgaccaat gttgtgcacg
     8941 ccggaagtac aggcggtcgc gatagagatg cttgggccac gcaggccata catgatagtc
     9001 agatgacctg ccaccatgtt cacaatcgtt gacggaacga agaatgggct gatcttacgt
     9061 ggaccaccgt tcatcagaga tgtgtggttt tcttcgatca gtccgaggcc gccaatcccg
     9121 gagccaattg cggcaccaat gcgggttgcg ttctcttccg ttatttcaag gccagaatcc
     9181 tgcatggcct gaacgccagc gacaattcca tattgaatga aggcatccat cttgcgctgt
     9241 tctttgcgcg agataatgtc ctcacagtta aaatccttta ctaagccagc aaatttcgtt
     9301 gcataggcgc tagtatcgaa atggtcgatt aggctgatgc cactctgacc ggcaagcaga
     9361 gctttccagg tagactctac ggtattgccg acaggagaca acatgcccag tccggtcaca
     9421 actacacgac gcttagacac gtttgtcctc cagggaggga aaaaatgatt ctagtgggac
     9481 aaaaagataa aactcaggcg gtcgaacgac cgcctggaga tgttcactta cgcctggtgg
     9541 ccgttgatgt aatcaatggc agcctgaacg gtggtgattt tctcagcttc ttcgtccgga
     9601 atctcagtat caaactcttc ttccagagcc attaccagct caacggtgtc aagagaatcc
     9661 gcgcccaggt cttcaacgaa agaagcattg ttggtaactt cttcctgctt aacgcccagc
     9721 tgttcgccga taattttctt aacgcgttct tcgatagtgc tcatactctt aaatttccta
     9781 tcaaaactcg ctttcgcgat ggttttcgta gtgtataaaa tgttgaaaaa tttgcaacta
     9841 aatcccggca ggtcttacca cgattttacg ttattttgag gccttacccc ccaataacgc
     9901 aaatcatttt gcactaatta ttactcataa ccacgcagac tgcgcgcaac atgagcattt
     9961 tgtgcaaatc gcggtcagac catgtacatc ccgccgttca catgcaaagt ttcacccgtg
    10021 atgtaagctg cttcgtcgga tgccaggaat gcaaccgcgt tggcgatttc ctgtgcgccg
    10081 ccgaggcgac ccgcaggaac ctgcgccagg atacccgcac gctggtcatc gctcagcgca
    10141 cgtgtcatgt ccgtttcaat aaagcccgga gcaacaacgt ttacagtaat accgcgtgac
    10201 gcaacttcgc gcgccagtga tttactgaag ccgatcaagc ccgctttcgc cgcagcgtag
    10261 ttggcctgac cgccatttcc catggtacca accacagaac cgatagtgat aatacgacca
    10321 tgacgctttt tcatcatagc gcgcattacc gcttttgaca gacggaaaac agatgaaagg
    10381 ttggtttcga taatatcgtt ccactcttca tctttcattc gcattaacag gttatcacga
    10441 gtgataccgg cattattgac caggatatcc acttcaccaa attctgcgcg aattttttcc
    10501 agaacagatt cgatagatgc cgggtcggtc acattcaaca tcagaccttt gccgttagca
    10561 cctaaataat cactgatcgc ctgagcgcca ttttcactgg tcgcagtgcc aataactttc
    10621 gcgccacggg ctgcgagcgt ttcagcaatt gcgcggccaa ttccgcggct tgcaccggtt
    10681 accagtgcga tttttccttc aaaattcatg attttcctct tttaaagctc gagcgccgct
    10741 gccatcgctg aaggttcgtt cagcgccgag gcggtcaggg tgtcgacaat gcgtttcgtc
    10801 aggccagtta gcactttgcc cgggccgact tcatagagat gttctacgcc ttgcgctgcc
    10861 atgtactcaa cagacttcgt ccactgaacc gggttataca actgacgtac cagtgcgtca
    10921 cgaatggcat caccattggt ttcgcatttc acatcaacgt tattcacaac aggaactgtt
    10981 ggtgcgttaa aggtgatttt cgctaattct actgccagtt tgtcggctgc tggtttcatc
    11041 agcgcacagt gagacggtac gctcactggt aacggcagcg cgcgttttgc gcccgcagct
    11101 ttacaggcag cgccagcacg ctcaaccgcc tctttatgac cggcaataac cacctgtccc
    11161 ggagagttaa agtttaccgg agaaacgacc tgaccttctg cagcttcttc acacgctttc
    11221 gcaatagacg catcatccag accgatgatt gccgccatag cgcccgtgcc ttctggtacg
    11281 gcttcttgca tgaacttgcc gcgcatctca accagacgca ccgcatcagc gaaatcaatc
    11341 acgccagcgc aaaccaacgc ggagtattcc cccaggctgt gaccggccat cattgccggt
    11401 gctttaccgc cctgctgctg ccatacgcga tacagcgcaa cagatgcagt caacagcgct
    11461 ggctgggttt gccaggtttt attcagttct tcagctggcc cctgctgggt cagcgcccac
    11521 aggtcgtagc ccagcgccgc agaagcttca gcaaacgttt cttcgacaat tggatagctc
    11581 gccgccatat cagccagcat tccaacggtt tgagaaccct gtccagggaa cacaaatgca
    11641 aattgcgtca tgttttaatc cttatcctag aaacgaacca gcgcggagcc ccaggtgaat
    11701 ccaccgccaa aggcttcaag cagaaccaac tgccccggct taatgcgccc gtcgcgtaca
    11761 gcttcatcca gcgcgcacgg gacagaggcc gcagaggtat taccgtggcg atccaacgtc
    11821 accacgacat tgtccataga cataccgagt tttttcgccg ttgcactgat aatacgcagg
    11881 ttagcctgat gcggaaccag ccagtccagt tgagaacggt caagattatt cgccgccagc
    11941 gtctcatcaa cgatgtgcgc cagttccgtt accgcaacct tgaagacttc gttgcccgcc
    12001 atcgtcagat gaattgaatt ctctggattc acgcggtcgg cgtttggcag cgtcagcaat
    12061 tcaccataac taccgtcggc atgcagatgg gtggagatga ttcccggctc ttcagaggca
    12121 gccagcaccg cagcgcccgc gccatcgcca aaaataataa tagtcccacg atctgttgga
    12181 tcgcaggtgc gcgccagtac atcggaaccg acgaccagag catacttcac cgccccagat
    12241 ttcacgtatt gattggctac gcttaatgca taggtgaaac ctgcgcaggc tgctgcaacg
    12301 tcaaatgccg ggcaaccttt aatgcccaac atgctttgaa tctgacaagc tgcgctcggg
    12361 aaagcgtgcg tagcagaagt cgttgccaca acgatcaggc caatctggtc tttctcaatg
    12421 cccgccatct caattgcgcg tgtcgccgct tcaaagccca tggttgaaac ggtttcgttt
    12481 ggcgcggcaa tgtggcgttc gcggataccg gtacgagtga caatccactc gtcagaggtg
    12541 tccaccattt tttccaaatc ggcgtttgtc cgcacttgtt cgggcagata gctgccagta
    12601 ccaataatct tcgtatacat gtacgctcag tcacttttcg gttatatacc gtcacttgca
    12661 aactgcgagt tcgctggcag cgtcctgcta ccgcagagtt ccgcttttgc caccgtccag
    12721 cagctcaaaa ccagctgggt atacagattc caggcgagcg gcaattcgct gaggaacttg
    12781 tcgctgcacc gcctgcactg cctgttcaat cgcgaccgca aaagctcgct gattggctgc
    12841 accatgactt tttatcaccg tgccgcgcaa tcctaacaga caggcgccgt tatactggtc
    12901 ggggttgagg tgactgaatc gcctcgtcag gctcttttgt agccaacgct ttaataacag
    12961 tagccaccac gaccgttttt tcccttcacc ctgagatttc agcagagaaa ggaacatcct
    13021 gacaacacct tccatcgtct ttaacgtgac atttcctgta aagccgtcac aaaccagcac
    13081 atctgtcttg ccagttaaca actcattggc ttcaagatag ccgatataat tgatagaagg
    13141 gattgtttta agcaccgctg aggcatcccg aatactgtcg agacccttta cttcttcttc
    13201 accaatattg agcaacgcca cgcgaggatt gggaatttcc accacctctt cagccagaac
    13261 tgagcccata atggcaaatt gcaccaacat tgtgctgtca caatcgacgt tggcccctaa
    13321 atcgaggacc accgttttgc ccttttgctg atgtggtaat accgtcacca gcgccggacg
    13381 ctcaatcccc tccaggggcc tgagtaataa ttttgccagc cccatcagtg ccccggtatt
    13441 accggcactg acacaggctt gcgctcgacc ttctttcacc agctccaggg ccatacgcat
    13501 tgaactccca cgactagcgc ggatagcttg cgaaggccgg gcatcactgg cgataactga
    13561 ctgcgcagga ataatctgca gacgcgaacg ttgttcaaag tcagctttag caagtaatgg
    13621 cgtgatggcg tcgggattgc cgactaaaag aagagtgagt tgcgaattag aattcagtgc
    13681 ctgcaatgct gcaggcactg tcacggaagg gccaaaatcc cctcccatga catctaacgc
    13741 cagggttaga cgtgtcaagg tatcgtcgcc gcccggtttg gtctttcccc agttgcctgg
    13801 ggaaaatcct cactaagctt catcacgcag acgcgtgatt acttagcgat gaccttgcgg
    13861 ccgcggtagt aaccgtcggc agtgatatgg tgacgcaggt gtttttcacc agaagttttg
    13921 tctacagaca ggctggtgac tgcggtcagc gcgtcatggg aacgacgcat gccacgtttg
    13981 gaacgggttg gtttattctg ttgtacggcc atggacctta ctcctcaatt acggtttatc
    14041 cccgatccaa cggggagcac caattactta cgctttaagc tggctaatac ggcaaatggg
    14101 tttggctttt gcacttcttc aggcagttca ccaaagacca tgtccgcttc ggacacttca
    14161 cagtgttcag aatcatgcac cggaactacc ggcaaggcga ggatgatttc atcttcaacc
    14221 attgcaagca gatcgatttc accgaattcg ttaacctcaa tcggttcata cgcttccggc
    14281 agtgcttcag cctgttcgtc tgaacgcaca ggactaaaac aatacgttgt gtagacctga
    14341 tgagtaaacg gcttcccgca acgctgacac tcgagcgtta ccgtcacctt cgcatcgccg
    14401 tttaacactg cgagacgttg gttatcgata gcgaacgaca tggagcattc cacatcactg
    14461 tccacactga ctacggattc ggcgacgcgc tcaacctgat caggggtata gataccctgg
    14521 taatcaaggc gtttttgagc cgtacgaacc ggatcgagag tcaggggtaa ttttaccttt
    14581 tgcatagggc gcgcatatta actttgtaac gtcatagagt caaagaaaaa ggcagcctgt
    14641 ggttgccttt tgccaataat tcgcacacat tgcgggttac tgctttattt tcgttcagtg
    14701 ctgataacga acatcaacct gcaaaacgaa gcgcatagtt taaaatggct atcatcaata
    14761 cgctatatct gatggaaaaa aatatgccta aacttatttt agcctccacc tcgccatggc
    14821 gtcgcgctct tctggaaaaa ctgcaaatct cttttgaatg tgcagcacca gaggtcgacg
    14881 aaaccccacg cagcgacgaa tcaccgcgac agttggtgct tcgactggca caagaaaaag
    14941 cgcaatctct ggcgtcacgt tatccggatc atttaattat tggttcagac caggtatgtg
    15001 ttcttgatgg cgaaatcacc ggtaaaccgt taacggaaga aaatgcccgt ctgcaattac
    15061 gcaaagccag cggcaatatc gtcaccttct atactggact ggcgttgttt aattcggcga
    15121 atgggcatct acaaacagaa gtggagcctt ttgacgtcca tttccgtcac ctgtgcgagg
    15181 cggagattga taattacgtg cgtaaggagc atcccctgca ctgcgcgggt agctttaaga
    15241 gtgaaggatt tggcattacg ctgtttgagc gtttagaggg acgtgaccct aacacgctgg
    15301 ttggtttacc gctaatcgcg ctgtgtcaga tgttacgtcg ggaagggaaa aacccgctga
    15361 tgggatagtt gtttgcgccg gatgcggtta cgtcgcatcc ggcaatcggt gcaggcggcc
    15421 tgatgcggcg taaacgcctt atcaggccaa ccgcccatta tcaggcttat atgcttgtat
    15481 tagcgcgcgt tacgcagctt ttgcaaacaa cgcttcaaac cttcatccat cggcgcttcg
    15541 atacgcatca cctcaccggt ccccggatgg gtaaacttca acgctgcggc gtgcaggaac
    15601 agacgattta atcccgtgcc tgcttcagtg agctgtctgt caaattcacg gtcaccgtag
    15661 cgatcgtcaa aggcaatcgg atgacccgca tactgtgtat gcacacggat ctgatgagta
    15721 cgtccggtta ccgggctaca acgcaccagg gtggcaaatg catagcgttc ttccacttta
    15781 aagcgtgttt ccgacggttt gccttcctga ctgacacgca cgatacgttc gccgctttgc
    15841 agaatatttt tcaataacgg cgcttgaacg ctcttcacat gcgactgcca ctgactgcgc
    15901 accagcgcca ggtaatcttt ttgcattcct ttttcacgta attgctcatg cagagaacgc
    15961 aacgccgagc gttttttcgc taccagcaac acacctgaag tgtcccggtc gagacgatga
    16021 accagttcaa ggaaccgcgc ttccgggcgc aacgcccgca aaccttcgat aacgccgaag
    16081 cttaaaccac tgccgccatg taccgccgta ccggaaggtt tattcagcac caggatatga
    16141 tcatcttcat ataagatgac gtccgccagc gccgccacct tttgcagatg tggcgaaacc
    16201 gcctcttctt cccgctcagc aacgcgaacc ggtgggatac gcacctcatc acccgcttcg
    16261 agtttatatt caggcttaat acgttttttg ttcacccgca cttcgccttt acgcaaaata
    16321 cggtaaatca tactttttgg tacgcctttc aattgggtac gcaaaaagtt gtcgatacgt
    16381 tgccccgctt cgtcagcggt gatagcaaca atttttacgg atggagtctc tgttttcatg
    16441 gtgggcgatt ctaattagcc aacaggattc gcgccactca tttttctatg cttatattta
    16501 gttttacacc ttattacttc actacgtgat cactttattg atggttatta aaccaatcac
    16561 cagcaagaag tgaaaaatgt aatgaacgca tcccacacaa ttaaacacgg agcagtatga
    16621 tggttaaaag ggtgaaactg gcaggtgtgg agcctcgcag atttccggca tcatagtgcc
    16681 caatacggga tgcataaagc tccctcagac tggagactcc acaaatgaaa tatacaccgg
    16741 ttggcgttg
//
LOCUS       DAJTMC010000085        16609 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.085, whole genome shotgun sequence.
ACCESSION   DAJTMC010000085 DAJTMC010000000
VERSION     DAJTMC010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16609
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.085"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(28..2217)
                     /locus_tag="OO130_002468"
     CDS             complement(28..2217)
                     /locus_tag="OO130_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
                     /protein_id="HCR5365837.1"
                     /translation="MFVEHNLIKNIKIFTLAFTLTVVLIQLSRFISPLAIIHSSYIFL
                     AWMPLCVMLSILFIFGWRGVVPVLCGMFCTNLWNFHLSFLQTAVMLGSQTFVVLCACA
                     ILRWQLGTRWRYGLTSRYVWQRLFWLGLVTPIGIKCSMYLVGSFFDFPLKISTFFGDA
                     DAIFTVVDLLSLFTAVLIYNMLFYYLTRMIVSPHFAQILWRRDIAPSLGKEKRAFTLS
                     WLAALSVLLLLLCTPYENDFIAGYLVPVFFIIFTLGVGKLRYPFLNLTWAVSTLCLLN
                     YNQNFLQGVETEYSLAFILAVLISFSVCLLYMVRIYHRSEWLNRRWHLQALTDPLTLL
                     PNFRALEQAPEQEAGKSFCCLRIDNLEFMSRHYGLMMRVHCIRSICRTLLPLMQENEK
                     LYQLPGSELLLVLSGPETEGRLQHMVNILNSRQIHWNNTGLDMGYGAAWGRFDGNQET
                     LQPLLGQLSWLAEQSCAHHHVLALDSREEMVSGQTTKQVLLLNTIRTALDQGDLLLYA
                     QPIRNKEGEGYDEILARLKYDGGIMTPDKFLPLIAQFNLSARFDLQVLESLLKWLATH
                     PCDKKGPRFSVNLMPLTLLQKNIAGRIIRLFKRYHISPQAVILEITEEQAFSNAESSM
                     YNIEQLHKFGFRIAIDDFGTGYANYERLKRLQADIIKIDGVFVKDIVTNTLDAMIVRS
                     ITDLAKAKSLSVVAEFVETQQQQALLHKLGVQYLQGYLIGRPQPLAD"
     gene            complement(2426..2501)
                     /locus_tag="OO130_002469"
     tRNA            complement(2426..2501)
                     /locus_tag="OO130_002469"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2466..2468),aa:Ala,seq:ggc)
     gene            complement(2541..2616)
                     /locus_tag="OO130_002470"
     tRNA            complement(2541..2616)
                     /locus_tag="OO130_002470"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2581..2583),aa:Ala,seq:ggc)
     gene            2837..3196
                     /locus_tag="OO130_002471"
     CDS             2837..3196
                     /locus_tag="OO130_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCR5365838.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            3198..3590
                     /locus_tag="OO130_002472"
     CDS             3198..3590
                     /locus_tag="OO130_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCR5365839.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGVRAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(3641..5056)
                     /gene="gltX"
                     /locus_tag="OO130_002473"
     CDS             complement(3641..5056)
                     /gene="gltX"
                     /locus_tag="OO130_002473"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HCR5365840.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGR
                     TRSIERINKALAFIAERENQQ"
     gene            5316..5391
                     /locus_tag="OO130_002474"
     tRNA            5316..5391
                     /locus_tag="OO130_002474"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5349..5351,aa:Val,seq:tac)
     gene            5436..5511
                     /locus_tag="OO130_002475"
     tRNA            5436..5511
                     /locus_tag="OO130_002475"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5469..5471,aa:Val,seq:tac)
     gene            5558..5633
                     /locus_tag="OO130_002476"
     tRNA            5558..5633
                     /locus_tag="OO130_002476"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5591..5593,aa:Val,seq:tac)
     gene            5638..5713
                     /locus_tag="OO130_002477"
     tRNA            5638..5713
                     /locus_tag="OO130_002477"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5671..5673,aa:Lys,seq:ttt)
     gene            5833..6174
                     /locus_tag="OO130_002478"
     CDS             5833..6174
                     /locus_tag="OO130_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HCR5365841.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASQIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQKQQAEKAQEKEQRLSLDVSLLNP
                     VEKTTHIDIYI"
     gene            complement(6165..7091)
                     /locus_tag="OO130_002479"
     CDS             complement(6165..7091)
                     /locus_tag="OO130_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5365842.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            7181..8179
                     /locus_tag="OO130_002480"
     CDS             7181..8179
                     /locus_tag="OO130_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HCR5365843.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCTLPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(8176..8394)
                     /locus_tag="OO130_002481"
     CDS             complement(8176..8394)
                     /locus_tag="OO130_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HCR5365844.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(8396..10411)
                     /gene="ligA"
                     /locus_tag="OO130_002482"
     CDS             complement(8396..10411)
                     /gene="ligA"
                     /locus_tag="OO130_002482"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HCR5365845.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIVGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(10482..11468)
                     /gene="zipA"
                     /locus_tag="OO130_002483"
     CDS             complement(10482..11468)
                     /gene="zipA"
                     /locus_tag="OO130_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HCR5365846.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPVYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            11698..12459
                     /gene="cysZ"
                     /locus_tag="OO130_002484"
     CDS             11698..12459
                     /gene="cysZ"
                     /locus_tag="OO130_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HCR5365847.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            12644..13615
                     /gene="cysK"
                     /locus_tag="OO130_002485"
     CDS             12644..13615
                     /gene="cysK"
                     /locus_tag="OO130_002485"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HCR5365848.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            13999..14256
                     /gene="ptsH"
                     /locus_tag="OO130_002486"
     CDS             13999..14256
                     /gene="ptsH"
                     /locus_tag="OO130_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HCR5365849.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            14301..16028
                     /gene="ptsI"
                     /locus_tag="OO130_002487"
     CDS             14301..16028
                     /gene="ptsI"
                     /locus_tag="OO130_002487"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HCR5365850.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            16069..16578
                     /gene="crr"
                     /locus_tag="OO130_002488"
     CDS             16069..16578
                     /gene="crr"
                     /locus_tag="OO130_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR5365851.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLPGSVTVG
                     ETPVIRIKK"
BASE COUNT         4196 a         4275 c         4073 g         4065 t
ORIGIN      
        1 gataattccc cgtttttttt acgcctgtta atcagctaat ggctgcgggc gaccaatcaa
       61 atacccttgc agatattgca ccccgagctt atgcaatagc gcctgctgct gttgcgtctc
      121 gacaaactcc gcgaccacac tcaatgactt cgctttcgcc agatcggtaa ttgatctcac
      181 aatcatcgca tccagcgtgt tcgtgacaat atctttcaca aagacgccat caattttgat
      241 gatatcagcc tgcaaacgct ttaaccgttc gtagttggca tagccggtgc caaagtcatc
      301 aatcgcaatc cggaaaccaa acttatgcag ctgctcgatg ttgtacatgc tgctttctgc
      361 gttagaaaac gcctgctcct cggtgatctc aagaatgacc gcctgcgggg agatgtgata
      421 acgcttaaac agacgaataa tccgcccggc aatattcttt tgcagcagcg tgagcggcat
      481 taaattgact gaaaagcgcg gtcctttttt gtcgcaaggg tgtgttgcca gccacttcaa
      541 caaggattcc agcacttgca aatcaaaacg cgcgctaagg ttaaactgag caataagggg
      601 cagaaactta tccggggtca taatgccgcc gtcatatttc agtcgcgcga ggatctcatc
      661 ataaccttca ccctctttgt tgcgaattgg ctgggcgtag agcagcaaat caccctgatc
      721 taacgccgtg cgaatggtat tcagcaatag cacctgttta gtggtctgcc cggaaaccat
      781 ctcctctctg ctatccagcg ccagcacatg atgatgtgcg caggattgct ccgccagcca
      841 gcttaactgc cccaacaagg gttgcagggt ttcctgattt ccatcaaaac gcccccaggc
      901 agcaccatag cccatatcca gcccggtatt gttccagtga atttgccgac tattcaggat
      961 gttaaccata tgctggagtc gcccttccgt ttccggcccg ctcagcacta acagcagttc
     1021 actacccggc aattgataca acttttcgtt ttcctgcatc aacggcagca gcgtacggca
     1081 aattgagcgg atacagtgaa cgcgcatcat taagccgtaa tgacgactca taaactcaag
     1141 attatcaatg cgcaggcagc aaaaactctt gcccgcctct tgctccggcg cttgttccaa
     1201 cgcacggaag ttgggcagga gcgttaacgg atcggtcagc gcctgcaaat gccagcggcg
     1261 attaagccat tcactgcgat gataaatgcg caccatatag agcaggcaaa cgctaaagga
     1321 aatcagcacc gcaagaataa atgccagcga atattcggtt tccacccctt gcaaaaagtt
     1381 ctggttgtaa ttcagaaggc aaagcgttga aacagcccag gtgagattta aaaacggata
     1441 gcgaagctta ccgaccccga gggtaaagat gatgaagaaa acgggtacca ggtaaccggc
     1501 aataaagtcg ttttcataag gtgtgcacaa cagaagtaga agcacgctta acgctgccag
     1561 ccagcttaag gtaaatgcgc gtttctcttt gcccaacgac ggcgcgatat ccctgcgcca
     1621 caatatctgc gcaaagtggg gacttacaat catgcgagtg agatagtaga aaagcatgtt
     1681 gtaaatcagc acagcggtga aaaggcttag caaatcaacg accgtgaaaa tggcatccgc
     1741 atcgccgaaa aaggtggata tctttagcgg gaaatcaaag aagctcccca caagatacat
     1801 gctgcatttg atgccgatcg gcgtcaccaa accaagccag aacagacgtt gccagacata
     1861 tcggctggtc aatccataac gccaacgcgt ccccagctgc cagcgtaata ttgcgcaggc
     1921 acacaacacg acaaacgtct ggctaccgag catgaccgca gtctgtaaaa aagagagatg
     1981 aaagttccac agattggtgc aaaacatccc gcataaaacg ggaacgacac ctcgccagcc
     2041 aaagataaac aagattgaca gcattacgca cagtggcatc cacgccagaa agatataact
     2101 ggaatggata atggcaagtg gcgaaataaa acgggatagc tgaataagta ccacggtgag
     2161 cgtaaacgct agtgtgaata tcttgatatt ttttatcagg ttatgctcca caaacatgag
     2221 tacactactc tcactgatcg gagttttcag gcacacaaat aataagttta ttcgcatcgc
     2281 gaagcaaaca gggggccagc ctgttgccga tttttcatcc aatgatgctt tcaccaggcg
     2341 gcactaaccc ggacgtagac tgtattacaa aagcggcaaa aagcagagac aaaaaacccc
     2401 cgctttgcag cgagggttgg aaatttggtg gagctaagcg ggatcgaacc gctgacctct
     2461 tgcatgccat gcaagcgctc tcccagctga gctatagccc cacgatgcgt ttacgtacca
     2521 aatttgctgg gtgcaaaatt tggtggagct aagcgggatc gaaccgctga cctcttgcat
     2581 gccatgcaag cgctctccca gctgagctat agccccgtca cgtaaagctt gtcgagttga
     2641 cgggcggcat catatgaatt ccgcccgaat gtgtcaacgg caaattgcaa cgtgtagttt
     2701 caatcgctga aaaatcaggc aaatgaacaa ttttggaaaa ccgctcgcat tcagtagtta
     2761 ttcatgtcac ggtttcctgt aaagtggtgt tataaaatga actactaata gacccacata
     2821 cattcaggga attgttatgt tcaaggagag gatgacacca gatgaacttg ccagactgac
     2881 cggctatagc cgccagacca ttaataaatg ggtacgcaag gaaggctgga caacgtcacc
     2941 aaaacctggc gtccagggtg gcaaagcccg actggttcac gtcaatgaac aggttcgtga
     3001 atatattcgc aatgctgaac gtccagaagg tcagggagaa gcgcctgccc tttctggtga
     3061 tgcgccgctt gaagtattgc tggtgacact ggcgaaagag atgacgccag ttgaacaaaa
     3121 acagtttaca tccttgcttc tgcgggaagg gattatcgga ttgttacaac gcttagggat
     3181 tcgcgatagc aaataatatg aaaagattac gcaataaaat gaccaccgaa gaactggctg
     3241 aatgcctcgg tgtggctaaa caaactgtta accgttggat cagagaaaaa ggctggaaaa
     3301 cggaaaaatt tcccggcgtg aaaggcggtc gtgccagact tattctggtc gatacgcaag
     3361 tttgcgagtt tattcagaac acgccagcct tccataacac accaatgttg atggaagctg
     3421 aagagagaat tgctgaatac gctcctggag tacgcgcccc ggcgtatcga caaatcatca
     3481 acgcgataga taatatgaca gacattgagc aggaaaaagt cgcgcaattt ttgtcacgtg
     3541 aaggaattcg caatttcctc gcccgtctcg atattgacga atcagcataa ataaaaaacg
     3601 gcaggatatt atctcctgcc gtttatcttt tatgcgctaa ttactgctgg ttttcgcgtt
     3661 cagcaataaa agccagcgct ttgttgatac gctcaatact gcgggtccta ccaattgcgt
     3721 gaacggtaac atccagtgcc ggagactgcc ccgcaccggt tacggcgaca cgcagcggca
     3781 taccaacttt acccataccc acttccagct catccgccgt cgcctgaata gcgtgatgaa
     3841 cgttttcagc ggtccagtca gtaatcgcgg ccagtttgtc acgaaccact tccagcggct
     3901 gacgcgctac cggacgcaga tgttttttcg cggcgtcggc atcgaactca gcaaaatctt
     3961 cgtagaaata acggcagctc tgtgccatct ctttcagcgt cttgcagcgt tcgcccagca
     4021 gtttcaccag atcagccagc tgcgggccgt tacgggtatc gatattttcc tgctcaatgt
     4081 gccactgtaa gtgagtagca acatactccg gcggcagcgc gttaatgtag tgatggttca
     4141 gccacagcag cttgtcggtg ttgaacgcac tggcagattt gctgacggca ttcagggtga
     4201 agtatttgat catctcttca cgagtgaaga tttcctgatc gccgtgggac cagcccagac
     4261 gcaccagata gttcagcagt gcttctggca aataaccgtc atcacgatac tgcattacgc
     4321 tgaccgcccc gtgacgtttg gacagttttt taccgtcatc gccgttgatc atagaaacgt
     4381 gcgcgtaaac cggcaccggc gctttcaggg ccttaagaat gttgatctgg cgtggcgtgt
     4441 tgttgatatg gtcttcgcca cggataacgt gggtgatttc catatcccag tcatcgacaa
     4501 ccacacagaa gttataggtt ggggaaccat cggtacggcg aataataaga tcgtccagtt
     4561 cctggttgct gaactcgatc ggaccacgga tctgatcgtc aaaaacaaca gaaccttcct
     4621 gcgggttagc aaaacgtaca acgcacggtt catcatcagc gtgatgctca tggctgtggc
     4681 ggcagcgacc gtcataacgc ggcttctcac ctttcgccat ttgctcttcg cgcagcgctt
     4741 ccaggcgctc tttagagcaa tagcatttat aagcagtgcc ctcttccagc atctgatcga
     4801 tcaccgcgtt gtagcgatca aaacgtttgg tctggtagta cggaccttca tcccactcca
     4861 ggctcagcca gttcatgcca tccataatgg cttcgatagc ttccggcgtg gaacgctcaa
     4921 gatcggtgtc ttcaatacgc agcacgaact caccgccgtg gttacgtgca aaaagccagg
     4981 agtaaagggc agtacgcgcg ccgccaacgt gcagatagcc tgttgggctt ggcgcgaagc
     5041 gagttttgat tttcatgaaa tggccttacg tttagaaaga tgccgacaac cggcaaatcc
     5101 tggaaaaatt gagtgggcga tattctatca ctcccgcctg attcctcaat gtagttcggg
     5161 tttctacctt acgcattatt gttttcgttg acaaattgcg caacacggtc tattttgcga
     5221 tcatcttgct taaatttacg acgaacgaac aatttcttta gaaaatgcgt tgactcattt
     5281 tgaactctcc ctataatgcg actccacaca gcggggggtg attagctcag ctgggagagc
     5341 acctccctta caaggagggg gtcggcggtt cgatcccgtc atcacccacc aactacttta
     5401 tgtagtctcc gccgtgtagc aagaaattga gaagtgggtg attagctcag ctgggagagc
     5461 acctccctta caaggagggg gtcggcggtt cgatcccgtc atcacccacc actttctcac
     5521 cagctaaatt tcttgtaaca atgtgaagta ccgaagtggg tgattagctc agctgggaga
     5581 gcacctccct tacaaggagg gggtcggcgg ttcgatcccg tcatcaccca ccacttcggg
     5641 tcgttagctc agttggtaga gcagttgact tttaatcaat tggtcgcagg ttcgaatcct
     5701 gcacgaccca ccaatgtaaa aaagcgccct aaaggcgctt ttttgctatc tgcgatttgt
     5761 aagattgatt tttcttctca agttccttgt tcttctaccg atattacctc gtacagtgac
     5821 gaggactgaa caatggccac tatctcttca acttcaattc cttccataca aacacagagt
     5881 agtaacaggg catcacaagg tagcgatgtt gcttcacaaa tcgcgcggat aagtcagcaa
     5941 atcatcaaac tcactcaaca aattaaagag attgtagaca ctagcggcag tgctgaagac
     6001 aagcaaaaac aggccgaatt gattcaacag caaatcaccc tactggaaac acaactggcg
     6061 cagttgcaga aacagcaggc agaaaaggcg caggagaaag agcagcgttt gtctctggat
     6121 gtatcccttc taaatcctgt tgaaaaaacg actcatatcg atatctatat ctgatacaac
     6181 ggatcccctt cccgaccagc cattagcgca ttgccctggt ctcgtaccac gtcccataac
     6241 gcttcagcgg cggtggaaag tgaacggttc ttcctgcgca ccagcatcaa ctggcgttca
     6301 acaaccggcg taatgcgctt taccaccagt gggctacctt caggcaatgg tagcgccagt
     6361 gcgggaagaa tactgatgcc gatgcctgcg gctaccatcg ggaacagcgt cgccggatgt
     6421 ccaatctcct gcacaatatt cgcctgaata ccattgcgcg ccagcgctgc gtcaatcagc
     6481 ggtcggctgc ctgacgcgta atcctgcaac accaatttcg ctccctgtaa tgcctgccag
     6541 ggtacataat cttccacggc caaagcgcta tcgcgatggc aaagcagaaa gaaaggctcg
     6601 gaaagaatcg cttcacattg cagatcgccc actgggcctg gatcgatgac gatgccaaag
     6661 tcgacatctc cctggcgaat actttccatc acccactgct gcggacgatc gtgcaggaca
     6721 aactgaatat ctggatagcg gcgatggctt tcagcaatgc attgcggaat aagatgcgcg
     6781 gaaatggtct ggctggcagc gacccgaact tttccgctca gttgttgccc catacgcccg
     6841 gtatcgcgca acgtgctgtt aagttcatcc agcagtcgct caagacgcaa agccaactgc
     6901 tgccctgcat ctgtaagcac cacttcacgc gtggttctgt ccagcaggcg aacaccagta
     6961 tgattttcca gctccttcac actgtgactt actgccgact ggctcaagcc gatacgctct
     7021 cctgcacgac taaaactttt ctcctgcgct actgtgacga aaacctttaa ttgttttaaa
     7081 gaataattca tctgttttct tcatgaatgg atgcaataaa tcaattttat ttctcaaact
     7141 ggaagaagca caatagaacc atcgatcatc tggagtcttt atgaaacttt ttcgtatcct
     7201 cgatcctttc accttaaccc tgatcacggt ggtgttgctg gcctctttct ttccggccag
     7261 aggcgatttc gtccccttct ttgaaaatct gaccaccgca gctattgccc tgctgttctt
     7321 tatgcacggc gcgaagttgt cgcgtgaggc gattattgct ggcggtggtc actggcgact
     7381 gcatttgtgg gtaatgtgca gcaccttcgt gctgtttccg attctgggtg tactgtttgc
     7441 ctggtggaaa ccggtaaatg tcgacccgat gctctactcc ggttttctct acttgtgcac
     7501 tctcccggct accgtgcagt ctgcaatcgc cttcacgtca atggcgggcg gtaacgtcgc
     7561 ggcggcggtt tgttctgcgt cagcatccag cctgctggga attttccttt caccattgct
     7621 ggttggtctg gtgatgaatg ttcacggtgc agggggcagc cttgagcagg tcggtaaaat
     7681 tatgctgcaa ctgctgctgc cgtttgtgtt ggggcatctt tcccggccgt ggattggtga
     7741 ctgggtgtcg cgcaataaaa aatggattgc gaaaactgac cagacgtcca ttctgttggt
     7801 ggtttataca gcgttcagcg aagccgtcgt taatggtatc tggcataaag ttggctgggg
     7861 atcattgctg tttatcgtgg tggtcagctg cgttcttctg gctatcgtga ttgtagttaa
     7921 cgtctttatg gcacgccgac tgggcttcaa taaggcagat gaaattacta tcgtcttttg
     7981 tggttcgaaa aagagtctgg caaatggcat cccgatggca aacattctgt tccccacatc
     8041 ggtgatcggt atgatggtgc tgcccctgat gattttccat cagatccaat tgatggtctg
     8101 tgcggtgctg gcgcgtcgat acaaacgcca gaccgaacag ttacaggcgc agcaggaaag
     8161 cagcgccgat aaagcttaaa gcggacgctt caggggctgg accagttgcg tcagcccctc
     8221 ggttttaatc agcagcgtga tttgcattag ctcaccgagc ttccctgccg ggaactcatc
     8281 tttgcgggca aaccacagca gatactcctc cggcaggtcg attaagcgac gccctttgta
     8341 tttgccaaac ggcattatcg tattggctat ttcaatcagc tgctcttttt ccatctcagc
     8401 tacccagcaa acgcagcatt tccgcttcgt cgatgacttc aatgcccagt tcctgcgcct
     8461 tcgccagttt agatcctgca gcttcaccca caatcaccag atcggttttc ttcgacacgc
     8521 tgcccgcgac tttcgcgccc agttcgacca gtcgagcttt agcgtcatca cgcgacatct
     8581 ggcttaagct gcccgtaagc accacggttt taccagcaaa cgggctgtca atctcttccg
     8641 cgttgataac gatcggcgca ggccagtgaa caccttccgc caacagctcg ctgatgacat
     8701 tgcggttgct ttcttcggca aagaagttgt gaacgtggga tgcaacgaca atgccaacat
     8761 caggcacctt ttgcagctct tcaatcgaag cggcttccag cgcttccagc gtgccgaaat
     8821 atgccgccag acctgctgcg gtggcctcgc cgacttcacg gatgccaagt gcatagagga
     8881 agcgagcaaa ggtggtttct ttcgcttttt ccagcgcgtt aaccacgttt tgtgccgatt
     8941 ttggccccat acgctccagt ccggtcagtt tgcctgcggt gagtttgaac agatctgccg
     9001 gagtgtggac atattctttt tcaaccagct gatcgatgat tttgtcgccc attccgtcaa
     9061 catccatcgc acggcgggaa acaaagtgtt tcagcgactc tttacgctgc gcaccgcaaa
     9121 tcaggccacc ggtacagcgg gcaaccgctt caccttccac acgctcaacg tcagaaccac
     9181 ataccggaca atgcgtcggg aatacaacct cacgggtatc ttccgggcgt tcagaaagca
     9241 cgacgttaac cacctgcggg atcacgtcgc cagcgcggcg aatcaccact ttatcgccaa
     9301 tgcgtaaacc aagacgttcg atttcatccg cattgtgtaa ggttgcgtta ctcaccagca
     9361 cgccagcaac atggacaggt tccagacgcg caacaggcgt aatcgcgcca gtacgcccaa
     9421 cctgaaactc gacgtcacgc acaaaggtca tctgctcctg cgccgggaat ttaaacgcta
     9481 ccgcccagcg cggggcacgc gcgacaaagc caagctgctc ctgctgttcc agtgagttga
     9541 ccttaatcac cacgccgtcg atatcaaagc ccagcgtcgg gcggtcttct tccactttgt
     9601 gatagaacgc cagcacttct tccgccgatt cacaaagcgt tacccgatcg ctgaccggca
     9661 acccccactt tttaaattgc agtaaacggc caagatgagt atccggcagc tcgccacctt
     9721 ccagaacacc aacgccatag cagaaaaaag tgagcggtcg cttcgctgta atacgcggat
     9781 caagctgacg cagtgaacca gctgccgcat tacgtgggtt agcaaaaact ttcccgcccg
     9841 tgcgtcgcgc atcttcgtta atcttttcga accccgcctg cggcaggaac acttcaccac
     9901 gcacttccag acgcgccggg atattctctc catgcagctt cagcggaatg gcgcgaatag
     9961 tacgcacatt agacgtgata tcttccccgg tggtgccatc gccacgggtc gcggcactga
    10021 ctaaaacgcc attttcatac agaatactga cggcaaggcc atccagcttc agctcacagc
    10081 accaggtgac tttctcgttg tttttcagac ggtcctgcac acgtttgttg aaagcaagaa
    10141 agctttcttc atcaaaaacg ttatccagtg acagcattgg tacttcatgg cgtatctggc
    10201 tgaaagccgc cagcggcgca gcacctacac gttgggtagg cgaatcaggc gtaatcagtt
    10261 ctggatgttt agtttccagc tcgcgcagtt cgcgcatcag cctgtcgtat tcagcgtcgg
    10321 gaatttccgg cgcatccatc acatgataaa gatattcatg atggcgaagc gtcgttcgca
    10381 gttctgtcag ttgttgttcg attgattcca tatcgcacca tcaatgctaa aaacccccga
    10441 caagcggggg ttcgaagaag agttaatttg ccttaagtgt atcaggcgtt ggcgtctttg
    10501 acttcgcgga tgatgtcctg gtactcgcgc aatttctgcg gagtcatcat acggcgctga
    10561 tcgtcaagca cgacaccgcc cacttcatcg gcaatatgct gcgcagattg cagcatcagc
    10621 ttgaagttct gcagctcgtc accgtaagac ggtacctgca taaagatagt gacacccgga
    10681 gtagtgaaat ccttcatttc aggatcaaag gttcccggtt tcaccatatt cgccaggctg
    10741 aataacgccg ggccgctgcc atccgggcta agatgacgat ggtaaatatt catatcgcca
    10801 aaaatgaagc ccgcttgttg aatgctgtta agaagcagtt caccgtttag ctcgctaccg
    10861 tgatgcgccg cgacgttcat gataatcacc gcttctttgc gcttcggttt atccataact
    10921 ggagccggtt ccgctacagg ctcaggctgt ggtgccgcta cgggttctgc aggctggaaa
    10981 gcctgttgtg ccggttgcgg tgctgaatgc acaggctgcg gcgctggcgc gacctgtggc
    11041 gaaactggct gctgcaacgg ctgttcaggc tgcggctgat agacaggctg ctgcaccggc
    11101 tgcgctggac gcggagcatg ttgcggcggt acctgcgctt caggcggctg ctggaccggt
    11161 tgacgcggct gcgcagacgc ataaggcggt tggtactggt gttgcggcga cggacgagca
    11221 gcctcatgct cctgagcgtt agccggggca tgattcacgc ggtgaacacg aacctcacca
    11281 acgccctcat catcttcgac atcctcgtca taagaatcgt cgtcacgttt tgacttcatt
    11341 cgttttaatg gccgatcgcg gaacatagaa gatcgttctt tacggctggt ccagaaacca
    11401 tgtaccagta aagcgattat ggcgatcgcg ccaacaatga ttaatatcag acgcaaatcc
    11461 tgcatcatta tattctctgt tgttctaaca ccttgccacc acggcaaaca tttactcact
    11521 aagagtattt gccgattacc tcaagtgcaa gtgcactatt aactttcaca gcacaaagat
    11581 agatgaaatc gtgctttttg ctgttttttc gaacatatcc taactgtcca ttgcgcaatt
    11641 acccggtaaa atacgcagaa ttttcctagg attggtcaaa aggagctcat cctgattatg
    11701 gtttcatcat tcacatctgc cccacgcagc ggtttttact attttgcgca aggctggaag
    11761 ctcgtctcgc aacctgggat tcggcgtttc gttattttac cgctgctggt caatattttg
    11821 ttgatggggg gcgcattctg gtggctcttt acacagctcg atgtctggat cccgactctc
    11881 atgagttacg ttccggactg gctacaatgg ctgagttatc tgttgtggcc tctggctgtc
    11941 atctctgtgc tgttagtgtt tggctatttc ttctccacga ttgctaactg gattgccgct
    12001 ccgtttaacg gtttattggc tgaacaactg gaagcacgat tgactggcgc tacaccgcca
    12061 gataccggga ttttcggtat catgaaagat gtgccgcgaa tcatgaaacg cgaatggcaa
    12121 aaatttgcct ggtatctgcc gcgcgcaatt gtattactaa ttctttactt catccctggt
    12181 attgggcaaa ccgtcgcgcc ggtactgtgg ttcctgttta gcgcctggat gttagccatc
    12241 cagtattgcg attacccctt cgataaccac aaagtgccgt ttaaagagat gcgcaccgcc
    12301 ctgcgcacac gcaaaatcac caatatgcag tttggtgctt taaccagcct gtttacgatg
    12361 atcccgctgc ttaatctgtt catcatgccc gttgccgttt gtggcgcgac ggcgatgtgg
    12421 gtcgattgct atcgcgataa acacgcgatg tggcggtaac aatctaccgg ttattttgta
    12481 aaccgtttgt gtgaaacagg ggtggcttat gccgcccctt attccatctt gcatgtcatt
    12541 atttcccttc tgtatataga tatgctaaat ccttacttcc gcatattctc tgagcgggta
    12601 tgctacctgt tgtatcccaa tttcatacag ttaaggacag gccatgagta agatttttga
    12661 agataactcg ctgactatcg gtcacacgcc gctggttcgc ctgaatcgca tcggtaacgg
    12721 acgcattctg gcgaaggtgg aatctcgtaa ccccagcttc agcgttaagt gccgtatcgg
    12781 tgccaacatg atttgggatg ccgaaaagcg cggcgtgctg aaaccaggcg ttgaactggt
    12841 tgaaccgacc agcggtaata ccgggattgc actggcctat gtagctgccg ctcgcggtta
    12901 caaactcacc ctgaccatgc cagaaaccat gagtattgaa cgccgcaagc tgctgaaagc
    12961 gttaggtgca aacctggtgc tgacggaagg tgctaaaggc atgaaaggcg caatccaaaa
    13021 agcagaagaa attgtcgcca gcaatccaga gaaatacctg ctgctgcaac aattcagcaa
    13081 tccggcaaac cctgaaattc acgaaaaaac caccggtccg gagatctggg aagataccga
    13141 cggtcaggtt gatgtattta ttgctggcgt tgggactggc ggtacgctga ctggcgtcag
    13201 ccgctacatt aaaggcacca aaggcaagac cgatcttatc tctgtcgccg ttgagccaac
    13261 cgattctcca gttatcgccc aggcgctggc aggtgaagag attaaacctg gcccgcataa
    13321 aattcagggt attggcgctg gttttatccc ggctaacctc gatctcaagc tggtcgataa
    13381 agtcattggc atcaccaatg aagaagccat ttctaccgcc cgtcgtctaa tggaagaaga
    13441 aggtattctt gcaggtatct cttctggagc agctgttgcc gcggcgttga aactacaaga
    13501 agatgaaagc tttaccaaca agaatattgt ggttattcta ccatcatcgg gtgagcgtta
    13561 tttaagcacc gcattgtttg ccgatctctt cactgagaaa gaattgcaac agtaatgcca
    13621 gcttgttaaa aatgcgtaaa aaagcacctt tttaggtgct tttttgtggc ctgcttcaaa
    13681 ctttcgcccc tcctggcatt gattcagcct gtcggaactg gtatttaacc agactaatta
    13741 ttttgatgcg cgaaattaat cgttacagga aaagccaaag ctgaatcgat tttatgattt
    13801 ggttcaattc ttcctttagc ggcataatgt ttaatgacgt acgaaacgtc agcggtcaac
    13861 acccgccagc aatggactgt attgcgctct tcgtgcgtcg cgtctattaa aaactggcgc
    13921 taacaataca ggctaaagtc gaaccgccag gctagacttt agttccacaa cactaaacct
    13981 ataagttggg gaaatacaat gttccagcaa gaagttacca ttacagctcc gaacggtctg
    14041 cacacccgcc ctgctgccca gtttgtaaaa gaagctaagg gcttcacttc tgaaattact
    14101 gtgacttcca acggcaaaag cgccagcgcg aaaagcctgt ttaaactgca gactctgggc
    14161 ctgactcaag gtaccgttgt gactatctcc gcagaaggcg aagacgagca aaaagcggtt
    14221 gaacatctgg ttaaactgat ggcggaactc gagtaatttc ccgggttctt ttaaaaatca
    14281 gtcacaagta aggtagggtt atgatttcag gcattttagc atccccgggt atcgctttcg
    14341 gtaaagctct gcttctgaaa gaagacgaaa ttgtcattga ccggaaaaaa atttctgccg
    14401 accaggttga tcaggaagtt gaacgttttc tgagcggtcg tgccaaggca tcagcccagc
    14461 tggaaacgat caaaacgaaa gctggtgaaa cgttcggtga agaaaaagaa gccatctttg
    14521 aagggcatat tatgctgctc gaagatgagg agctggagca ggaaatcata gccctgatta
    14581 aagataaaca catgacagct gacgcagctg ctcatgaagt tatcgaaggt caggcttctg
    14641 ccctggaaga gctggatgat gaatacctga aagaacgtgc ggctgacgta cgtgatatcg
    14701 gtaagcgcct gctgcgcaac atcctgggcc tgaagattat cgacctgagc gccattcagg
    14761 atgaagtcat tctggttgcc gctgacctga cgccgtccga aaccgcacag ctgaacctga
    14821 agaaggtgct gggtttcatc accgacgcgg gtggccgtac ttcccacacc tctatcatgg
    14881 cgcgttctct ggaactgcct gctatcgtgg gtaccggtag cgtcacctct caggtgaaaa
    14941 atgacgacta tctgattctg gatgccgtaa ataatcaggt ttacgtcaat ccaaccaacg
    15001 aagttattga taaaatgcgt gctgttcagg agcaagtggc ttctgaaaaa gcagagcttg
    15061 ctaaactgaa agatctgcca gctattacgc tggacggtca ccaggtagaa gtatgcgcta
    15121 acattggtac ggttcgtgac gttgaaggtg cagagcgtaa cggcgctgaa ggcgttggtc
    15181 tgtatcgtac tgagttccta ttcatggacc gcgacgcgct gcccactgaa gaagaacagt
    15241 ttgctgctta caaagcagtg gctgaagcgt gtggctcgca ggcggttatc gttcgtacca
    15301 tggacatcgg cggcgacaaa gagctgccat acatgaactt cccgaaagaa gagaacccgt
    15361 tcctcggctg gcgcgctatc cgtatcgcga tggatcgtaa agagatcctg cgcgatcaac
    15421 tccgcgctat cctgcgtgcc tcggctttcg gtaaattgcg cattatgttc ccgatgatca
    15481 tctctgttga agaagtgcgt gcactgcgca aagagatcga aatctacaaa caggaactgc
    15541 gcgacgaagg taaagcgttt gacgagtcaa ttgaaatcgg cgtaatggtg gaaacaccgg
    15601 ctgccgcaac aattgcacgt catttagcca aagaagttga tttctttagt atcggcacca
    15661 atgatttaac gcagtacact ctggcagttg accgtggtaa tgatatgatt tcacaccttt
    15721 accagccaat gtcaccgtcc gtgctgaact tgatcaagca agttattgat gcttctcatg
    15781 ctgaaggcaa atggactggc atgtgtggtg agcttgctgg cgatgaacgt gctacacttc
    15841 tgttgctggg gatgggtctg gacgaattct ctatgagcgc catttctatc ccgcgcatta
    15901 agaagattat ccgtaacacg aacttcgaag atgcgaaggt gttagcagag caggctcttg
    15961 ctcaaccgac aacggacgag ttaatgacgc tggttaacaa gttcattgaa gaaaaaacaa
    16021 tctgctaatc cacgagatgc ggcccaattt actgcttagg agaagatcat gggtttgttc
    16081 gataaactga aatctctggt ttccgacgac aagaaggata ccggaactat tgagatcatt
    16141 gctccgctct ctggcgagat cgtcaatatc gaagacgtgc cggatgtcgt ttttgcggaa
    16201 aaaatcgttg gtgatggtat tgctatcaaa ccaacgggta acaaaatggt cgcgcctgta
    16261 gacggtacca ttggtaaaat ctttgaaacc aatcacgcgt tctctatcga atctgatagc
    16321 ggcgttgaac tgttcgtcca cttcggtatc gacaccgttg aactgaaagg cgaaggcttc
    16381 aagcgtattg cagaagaagg tcagcgcgtg aaagttggcg atactgtcat tgaatttgat
    16441 ctgccgctgc tggaagagaa agccaagtct accctgactc cggttgttat ctccaacatg
    16501 gacgaaatca aagaactgat caaactgccc ggtagcgtaa ccgtgggtga aaccccggtt
    16561 atccgcatca agaagtaatt cttgccgcag tgaaaaatgg cgcccatcg
//
LOCUS       DAJTMC010000086        16566 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.086, whole genome shotgun sequence.
ACCESSION   DAJTMC010000086 DAJTMC010000000
VERSION     DAJTMC010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16566
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.086"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            4..1104
                     /gene="arnB"
                     /locus_tag="OO130_002489"
     CDS             4..1104
                     /gene="arnB"
                     /locus_tag="OO130_002489"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HCR5365852.1"
                     /translation="MEELAAVKEVLESGWITTGPKNQALEQAFCQLTGNQHAIAVSSA
                     TAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDVDRDTLMVTPEAIE
                     SAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVGTYYKGRHIGAKGT
                     AIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDRQTWGRAPQAEVLT
                     PGYKYNLTDINAAIALTQLAKLEHLNTRRREIAQQYQQALAALPFQPLSLPAWPHVHA
                     WHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYRERFPTLSLPNTEW
                     NSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            1108..2076
                     /gene="arnC"
                     /locus_tag="OO130_002490"
     CDS             1108..2076
                     /gene="arnC"
                     /locus_tag="OO130_002490"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HCR5365853.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            2076..4058
                     /gene="arnA"
                     /locus_tag="OO130_002491"
     CDS             2076..4058
                     /gene="arnA"
                     /locus_tag="OO130_002491"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HCR5365854.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGS
                     VAHLAAERGIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHATRQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISIAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQTLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NAHRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            4055..4945
                     /locus_tag="OO130_002492"
     CDS             4055..4945
                     /locus_tag="OO130_002492"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HCR5365855.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKTEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            4945..6597
                     /gene="arnT"
                     /locus_tag="OO130_002493"
     CDS             4945..6597
                     /gene="arnT"
                     /locus_tag="OO130_002493"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HCR5365856.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFASLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            6594..6929
                     /gene="arnE"
                     /locus_tag="OO130_002494"
     CDS             6594..6929
                     /gene="arnE"
                     /locus_tag="OO130_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HCR5365857.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            6929..7315
                     /gene="arnF"
                     /locus_tag="OO130_002495"
     CDS             6929..7315
                     /gene="arnF"
                     /locus_tag="OO130_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HCR5365858.1"
                     /translation="MGLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(7309..7575)
                     /gene="pmrD"
                     /locus_tag="OO130_002496"
     CDS             complement(7309..7575)
                     /gene="pmrD"
                     /locus_tag="OO130_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HCR5365859.1"
                     /translation="MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCTAAGKTQ"
     gene            complement(7685..9040)
                     /gene="menE"
                     /locus_tag="OO130_002497"
     CDS             complement(7685..9040)
                     /gene="menE"
                     /locus_tag="OO130_002497"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HCR5365860.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQY
                     ALVLEGENTFPALTSLHIQMVEGTHSALWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLLFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(9037..9999)
                     /gene="menC"
                     /locus_tag="OO130_002498"
     CDS             complement(9037..9999)
                     /gene="menC"
                     /locus_tag="OO130_002498"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HCR5365861.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQSVLLAWVNNWLAGDCELLQMPSVAFGVSCALAELADTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALRLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSTLPVVEVDALERLL"
     gene            complement(9999..10856)
                     /gene="menB"
                     /locus_tag="OO130_002499"
     CDS             complement(9999..10856)
                     /gene="menB"
                     /locus_tag="OO130_002499"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HCR5365862.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNVGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(10871..11629)
                     /gene="menH"
                     /locus_tag="OO130_002500"
     CDS             complement(10871..11629)
                     /gene="menH"
                     /locus_tag="OO130_002500"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HCR5365863.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLAG
                     LCGVIVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(11626..13296)
                     /gene="menD"
                     /locus_tag="OO130_002501"
     CDS             complement(11626..13296)
                     /gene="menD"
                     /locus_tag="OO130_002501"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HCR5365864.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPSLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(13385..14680)
                     /gene="menF"
                     /locus_tag="OO130_002502"
     CDS             complement(13385..14680)
                     /gene="menF"
                     /locus_tag="OO130_002502"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HCR5365865.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLEEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(14759..15064)
                     /gene="elaB"
                     /locus_tag="OO130_002503"
     CDS             complement(14759..15064)
                     /gene="elaB"
                     /locus_tag="OO130_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HCR5365866.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQVSDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(15119..15580)
                     /locus_tag="OO130_002504"
     CDS             complement(15119..15580)
                     /locus_tag="OO130_002504"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5365867.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLDT
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            15645..16562
                     /gene="rbn"
                     /locus_tag="OO130_002505"
     CDS             15645..16562
                     /gene="rbn"
                     /locus_tag="OO130_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HCR5365868.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
BASE COUNT         3819 a         4507 c         4257 g         3983 t
ORIGIN      
        1 ggcgtggagg aactcgctgc agttaaagaa gttctcgaat ccggttggat cacaaccggt
       61 ccgaagaatc aggcgcttga gcaagccttt tgccagttga cgggaaatca gcatgccatc
      121 gcggtcagtt cagccaccgc cggaatgcat atcacgctaa tggcgttgga aattggcaag
      181 ggcgatgaag tgattacgcc ttccctgacc tgggtttcaa ccctcaatat gatttccttg
      241 ttgggtgcaa cgccggtaat ggtggatgtc gaccgcgata cgctgatggt cacgcctgaa
      301 gcgatcgagt cagccattac gccacgcact aaagccatca ttccggtgca ttatgccggt
      361 gcgccagcag atattgacgc cattcgcgcc attggcgaac gttacggcat cgcagttatc
      421 gaagatgctg cccatgccgt cggtacgtat tacaaagggc gacatattgg cgcaaaaggt
      481 accgctattt tttcatttca tgccattaaa aatattacct gtgcggaagg tggcctgatt
      541 gtaactgata atgaaaacct tgcccgccag ctacggatgc tgaaatttca cggcctgggt
      601 gtcgatgcct acgacagaca aacctggggc cgagcaccgc aggctgaagt cttaacaccg
      661 ggctataagt acaatctgac cgatattaac gccgcaattg ccctgacaca gttagcaaaa
      721 ttagagcacc tcaacacccg tcggcgcgaa attgcccagc aatatcagca agcactggca
      781 gctctcccct ttcagccatt aagccttccc gcctggccgc acgttcacgc ctggcatctg
      841 tttattattc gtgtcgatga acaacgttgt ggtatcagtc gcgatgcgtt gatggaagcg
      901 ttaaaagaaa gaggcattgg taccgggtta catttccgcg ccgctcacac acaaaaatat
      961 tatcgcgagc gttttcccac gctgtcgtta ccgaataccg aatggaatag cgaacgcatc
     1021 tgttctttgc cgctgttccc ggatatgact accgccgatg ccgaccgtgt catcacagcc
     1081 cttcagcagc tcgcaggaca ataagccatg tttgaaatcc accctgttaa gaaagtctcg
     1141 gtggttattc ccgtttataa cgagcaggaa agcttaccgg aattaatcag gcgcaccacc
     1201 acagcctgtg aatcgttggg gaaagagtat gagatcctgc tgattgatga cggcagtagc
     1261 gataattccg cgcatatgct ggtcgaagcc tcacaagcgg agaacagcca tattgtgtct
     1321 attttgctta accgcaatta cgggcaacat tcagcgatta tggcgggatt cagtcacgtt
     1381 actggcgact taattattac ccttgatgcc gatctccaga atccgccaga agaaatcccc
     1441 cgcctagtgg caaaagccga tgaaggttac gacgtggtag ggactgtacg ccagaaccgc
     1501 caggacagct ggtttcgtaa aacagcttcg aagatgatta accggcttat tcagcgcacc
     1561 accggcaaag cgatgggtga ctacggttgt atgctgcgcg cctatcgccg tcatattgtc
     1621 gatgcgatgt tgcactgcca tgaacgtagc acctttatcc cgattctggc gaatatcttc
     1681 gcccgccgtg ccattgaaat tccagtacat catgccgagc gtgagtttgg tgaatccaaa
     1741 tacagtttta tgcgcctgat taatttgatg tacgacctgg tgacctgcct taccacaacg
     1801 ccgctacgta tgctgagtct gctcggcagc attattgcga ttggaggttt tagcattgcg
     1861 gtgctgctgg tgattttacg cctgaccttc ggaccacaat gggcggcaga aggcgtcttt
     1921 atgctatttg ccgtgctgtt tacttttatt ggcgctcagt ttatcggcat gggattactc
     1981 ggtgaatata tcggcaggat ctacaccgat gtccgcgccc gcccccgcta ttttgttcag
     2041 caagttatcc gtccatccag caaggaaaat gaataatgaa aaccgtcgtt tttgcctacc
     2101 acgatatggg atgcctcggt attgaagctc tgctggctgc cggttacgaa attagcgcca
     2161 tttttaccca taccgataat cccggtgaaa aagcctttta tggttcggtg gctcatctgg
     2221 cggcggaaag aggcattccg gtttatgcgc cggataacgt taatcatccg ctgtgggtgg
     2281 aacgcattgc ccaactgtca ccagaggtga ttttctcttt ttattatcgc catcttattt
     2341 gcgacgagat tttgcagctc gctcccgcag gtgcatttaa tctgcacggt tcgctgttac
     2401 caaaatatcg tggtcgcgcg ccgctgaact gggtgctggt gaacggtgaa acggaaacgg
     2461 gcgttacatt gcaccgaatg gtaaaacgtg ccgatgctgg ggccattgtg gcgcaactgc
     2521 gcgttgccat tgcgccagac gatatcgcta ttacgctgca tcataaattg tgccatgcca
     2581 cgcgccagct actggaacag acattacccg ccattaaaca cggtaatatt ctggaaatcg
     2641 cccagcgcga aaacgaagcc acctgttttg gtcgcagaac gccggatgac agtttccttg
     2701 aatggcataa accggcatcc gtactgcaca acatggtacg tgccgttgcc gatccgtggc
     2761 cgggtgcctt cagctatgtt ggcaatcaga aattcaccgt ctggtcgtcg cgtgttcatc
     2821 ctcatgccag caaagcacag ccggggagcg tgatttctat tgcaccgctg ttgattgcct
     2881 gtggcgatgg cgcgctggaa atcgtcaccg gacaggcggg cgacggcatt actatgcagg
     2941 gctcgcaatt agcgcagacg ctgggcctgg tgcaaggttc acgcttgaat agccagcctg
     3001 cctgcaccgc ccgacgccgt acccgggtac tcatcctcgg ggtgaatggc tttattggca
     3061 accatctgac agaacgcctg ctgcgcgaag atcattatga agtttacggt ctggatattg
     3121 gcagcgatgc gataagccgt tttctgaatc atccgcattt tcactttgtt gaaggcgata
     3181 tcagtattca ttccgaatgg attgagtatc atgtcaaaaa atgtgatgtc gtcttgccgc
     3241 tggtggcgat agccacgccg attgaatata cccgcaaccc gctgcgcgta tttgaactcg
     3301 attttgaaga gaatctgcgc attatccgct actgcgtgaa gtaccgtaag cgaatcatct
     3361 tcccgtcaac ttcagaagtt tatgggatgt gtagcgataa atacttcgat gaggaccatt
     3421 ctaatttaat cgtcggcccg gtgaataaac cacgctggat ttattcggta tcaaaacaat
     3481 tacttgatcg ggtgatctgg gcctatggcg aaaaagaggg tttacagttc accctcttcc
     3541 gcccgtttaa ctggatggga ccacgactgg ataaccttaa tgcggcacga atcggcagct
     3601 cccgcgctat tacgcaactc attctcaatc tggtagaagg ttcaccgatt aagctgattg
     3661 atggcggaaa acaaaaacgc tgctttactg atattcgcga tggtatcgag gcgttatacc
     3721 gcattatcga aaatgcggga aatcgctgcg acggtgaaat tatcaacatt ggcaatcctg
     3781 agaacgaagc gagcattgag gaactgggcg agatgctgct ggcgagcttc gaaaaacatc
     3841 cgctgcgcca tcatttccca ccgtttgcgg gctttcgcgt tgtcgaaagt agcagctact
     3901 acggcaaagg atatcaggac gtagagcatc gtaaaccgag catccgcaat gcccaccgct
     3961 gcctggactg ggagccgaaa attgatatgc aggaaaccat cgacgaaacg ctggatttct
     4021 tcctgcgcac cgttgatctt acggataaac catcatgacc aaagtaggct tacgcattga
     4081 tgtcgatacc tttcgtggca cccgtgaagg cgtgccgcgt ctgctggaaa tcttgagtaa
     4141 gcataatatt caggccagca tttttttcag cgtcggcccg gacaatatgg gccgccatct
     4201 ctggcgactg gtgaagccac agtttttgtg gaagatgctg cgctcaaacg cggcatcgct
     4261 ttatggctgg gatattttac tggcaggtac ggcctggcca ggtaaagaga ttggtcatgc
     4321 caatgccgat atcattcgtg aagcggctaa acatcacgaa gtcggcctgc acgcctggga
     4381 tcaccatgcc tggcaagccc gtagcggtaa ctgggatcgg caaacaatga tcgacgatat
     4441 tgcacgcggt cttcgcactc tggaagagat tatcggtcaa ccggtaacct gttctgccgc
     4501 tgcgggctgg cgtgccgacc agaaggtgat cgaagcaaaa gaagcgttcc atttgcgcta
     4561 caacagcgat tgtcgtgggg ccatgccgtt ccgtccattg ctcgaatcag gaaaccctgg
     4621 cactgcgcaa attccggtga ccttacccac ctgggatgaa gtgattggtc gggatgtgaa
     4681 aacagaagat tttaacggtt ggttactcaa ccgcatcctg cgagataaag gcacgccggt
     4741 ttataccatt catgcagaag ttgaaggctg cgcttatcag cataattttg tggatctcct
     4801 caaacgcgca gctcaggaag gcgtgacatt ttgcccttta agcgaactgt tatcagagac
     4861 gttgccgctc ggacaagttg ttcgcggaaa tattgccgga cgtgaaggct ggctgggttg
     4921 ccaacaaatt gcgggtagtc gctgatgaaa tcggtacgtt accttatcgg cctcttcgcg
     4981 tttattgcct gctattacct gttaccgatc agcacgcgtc tgctctggca acccgatgaa
     5041 acgcgttatg cggaaatcag tcgagaaatg ctagcatccg gcgactggat tgtgccccat
     5101 ctgttagggc tacgttattt cgaaaaaccc attgccggat actggattaa cagcattggg
     5161 caatggctat ttggcgcgaa taactttggt gtgcgggcag gcgttatctt tgcgaccctg
     5221 ttaactgccg cgctggtgac ctggtttact ctgcgcttat ggcgcgataa acgtctggct
     5281 ctactcgcca cagtaattta tctctcattg tttattgtct atgccatcgg cacttatgcc
     5341 gtgctcgatc cgtttattgc attctggctg gtggcgggaa tgtgcagctt ctggctggca
     5401 atgcaggcac agacgtggaa aggcaaaagc gcaggatttt tactgctggg aatcacctgc
     5461 ggcatggggg tgatgaccaa aggttttctc gcccttgccg tgccggtatt aagcgtgctg
     5521 ccatgggtag cgacgcaaaa acgctggaaa gatctcttta tttacggctg gctggcggtt
     5581 atcagttgcg tactgacggt tctcccttgg ggactggcga tagcgcagcg ggagcctgac
     5641 ttctggcact attttttctg ggttgagcat attcaacgct ttgcactgga tgatgcccaa
     5701 catagagctc cgttctggta ctacgtgccg gtcatcattg ccggtagcct gccgtggctg
     5761 ggattactcc ccggtgcact gtacacaggc tggaaaaacc gcaagcattc cgcaaccgtc
     5821 tatttgttga gctggacgat aatgccgctg ctgtttttct ccgtcgctaa aggtaaattg
     5881 cccacctata ttctttcctg ctttgcatct ctggcaatgc tgatggcgca ttacgctttg
     5941 ctggcagcaa aaaataatcc tctggcgctg cggattaatg gctggattaa catcgctttt
     6001 ggcgtcactg gcattattgc cacatttgtg gtctccccgt ggggaccaat gaacacgccg
     6061 gtgtggcaaa ccttcgagag ctataaagtc ttttgtgcct ggtcgatttt ttcgctatgg
     6121 gcatttttcg gctggtacac cttaacaaac gtcgaaaaga cctggtcttt cgccgcgctt
     6181 tgcccgctgg ggctggcgtt gctggtagga ttttcaattc ctgacagagt catggaagga
     6241 aaacatccgc aattttttgt cgagatgaca caagaatcac ttcaaccaag ccgctatatt
     6301 cttactgata gcgtcggtgt tgccgcaggt ctggcatgga gcctgcaacg cgatgacatc
     6361 atcatgtatc gccagacagg tgagttgaaa tacggcctta attatccgga tgcgaaaggg
     6421 agatttgtca gcggtgatga gttcgcaaac tggcttaatc aacatcgtca ggaggggatt
     6481 attacactcg tgctttcggt tgaccgcgat gaagatatca acagtctcgc cattccgccc
     6541 gcagatgcca tcgatcgtca ggagcgtctg gtgctgattc agtatcgtcc caaatgatct
     6601 ggctaacatt agtctttgcc agcttgctta gcgttgccgg gcagttgtgt cagaaacagg
     6661 caacctgctt tgtggcgata aacaaacggc gcaaacatat cgtgctgtgg ctgggactgg
     6721 cgctggcttg tcttggtctt gccatggtgc tctggctgct ggtcttgcag aacgtaccgg
     6781 taggcattgc ctacccgatg ttaagtctga attttgtctg ggtgacgctg gctgcagtaa
     6841 aactgtggca cgaaccggta tcgccgcgtc actggtgtgg ggtggcgttc attattggtg
     6901 gcattgtgat cctcgggagt acggtgtaat gggcctgata tggggattat tcagcgtcat
     6961 tattgcctca gttgcgcaat taagcctggg ttttgcggca agtcatctgc cgccgatgac
     7021 gcacttatgg gattttatcg cggctctgtt agcttttggc ctggatgccc ggatactgtt
     7081 actcgggctg ctgggttatc tgctttccgt tttttgttgg tataaaacgc tacataaact
     7141 tgcgctaagc aaagcctatg ccttgctcag tatgagttat gtgctggtat ggattgcttc
     7201 aatggtactt cccggctggg aagggacttt ttcgctgaaa gcactactgg gagtagcctg
     7261 tattatgagc gggttgatgc tgattttcct gcccacgaca aaacaacgtt actgagtttt
     7321 ccctgccgct gtacactgcc gttcccactc atcggggcta taactgcttg ccgagaggac
     7381 tttcaccgta tgcagctttt cacgattaat acaataaagc gcattctgca aaggcgagag
     7441 taaatccccc actttcacgg cgaagtcact cttcacttcg gcgatcattt ttatcgcgcc
     7501 gccagcatcg cagagcataa gtacatgtct gttatcttgt ttattgcagc acgatttttt
     7561 gaccagccat tccattgcat tatcctgttt gctaagagtt ttcacatcaa tttacagatt
     7621 aacagataaa ctctttatca acggcaatgg aatggcagaa atgagaagtg aaacggaggg
     7681 ttaattattg ctgacgttgc acccactctt ttagcgcctg acgtgaaatt ttaataccgc
     7741 cgtttttcag ctccggcggt aaagtcagcc agcgcaccgg ttgttgaaaa cgagccagct
     7801 tatctttcac ccattcgcca agatcaacgg tttgctggtc atactccacc accgctaccg
     7861 gccgatgacc aaactccttg tcggcaacgg gaacgataaa cacctgcaaa accgcaggat
     7921 gtgcagcaat aacacgctcg acttcttccg gctgaatacc ctcaccgcca ctgaataaca
     7981 gattgtctaa acgtccgaca atggtcagct tgccattatg catctcaccg cgatcgcgcg
     8041 tagcgtacca gccttcgtca ttaaccagtg aaaccagttg cccgttacgc cagtaacctt
     8101 ctgccatact ggcagcccgc agccacactt cattattaac gattttcact tcccgacccg
     8161 gcagcggcga accaacgtct gccaggccgt cggcttcttt cgcacatacc gtggaggcaa
     8221 actcggtcag accatagccg caaaagcaac gaatcccctg ctcgcgcgcc tgttccgtca
     8281 actcgaccgg gatagccgcg ccgccaagta acaccgcttt cagggaaacg gaactgcggt
     8341 taaccagcaa acgccagagt tgtgttggca ccagtgaagc gtgagtacag cctgccagca
     8401 tttgctccaa tggctgttta tcacgcaccg tcatccgcgc accagcgtat aaccagcgcc
     8461 acataattcc ctgaccggag acgtgaaaca gcggtaaaga gagcaaccag tcatcgtgat
     8521 cgccaaacgg aatcagcgat aacacacctt gcgcactggc aagatgggcc tgataagtat
     8581 ggacagcggc tttcggtaaa ccggtggagc cggaggtcaa tgtcattgaa caaagacgtg
     8641 ttggttgcca cagagctgaa tgtgtgcctt caaccatctg aatatgcagt gaagttaacg
     8701 ccgggaaagt attttctcct tccagcacca gcgcatattg cagtgtaaga tttggcagta
     8761 attcctcaag caacggttgc ggaagctgtg gatttacggg cagcacccgc gccccgcatt
     8821 gcagtaacgc cagccaggcg agcagcgttt gcggcgtatt ccacgcccgc aacatcacgc
     8881 cgctgccctc aaccacaccc tgcgccgcaa agcccgtagc taactcatcg acgcgagcac
     8941 aaagctcgcg ccagttgagt tgctcgtcat taagacgtaa ggcgatggct tctccccgca
     9001 cttgccgcca gtgacgccac ggccagtcag agaagatcat aacaaccgct ccagtgcatc
     9061 aacttccacg acaggcagcg tgctacccgg ccagcgacgt acctgctgcg cctgcatcag
     9121 atccagcgtg tccagccctg gaatggtgtc cggcgttaac caggcggcaa tccgcgccag
     9181 ttgcgttaag cctaagctcg actcaataga agaactgatc accgccgtca gcctcaacgc
     9241 gtgcgccgcc tgtacctgct cgcgtacttt ttccagactg cccgtgagcg tgggtttgat
     9301 aactaccgcg cgcacgccct cttcagccac aaaggcaaaa tccggctcgc gcaggctttc
     9361 atcccaggca atggcaatgc cggtttcacg ggcaaacgct cgcgaatcat cgcgggtttt
     9421 gcacggctct tcgagaaacg cgatgcggtg gcgataatcc gggttaacat atttggcaaa
     9481 ctgctgacct ttcagcggtg tccaggcgcg atttgcgtca agacgcaaat gcagatccgg
     9541 aattgcctcc agcaacagat taaccaccat gccgtcgcgc acggcttcgt acaatcccac
     9601 tttgaccttc gccactttct cgcctggcat atcagcaagt ttgaggatca gatcgtcagg
     9661 atcgccatta cacagtggtg ccgcacggta attggccgcc tgcgggagcg tatctgccag
     9721 ctccgccaat gcacagctta cgccaaaggc aacggaaggc atctgcagta gctcgcaatc
     9781 gcctgccagc cagttattta cccaggcaag cagcacgctt tgcgcctctt cccaggtttc
     9841 ctggctgaag cccggcagtg gggagatctc cccccaccct tcgcgctcgc cctcacgcag
     9901 gcaaacgtac agcccgtcgc gggtttttaa ccgcctgtcg cgcagaacca cccccgcgtc
     9961 catggggatc tgccagcggt atacctgcgc gctacgcatt acggattccg tttaaatttg
    10021 ctgaaatcag gctgacgttt ctggttgaaa gcgttgcgac cttcctgacc ttcttccgtc
    10081 atgtagaaca gcatggtggc gttgcccgcc agctcctgca gccccgcctg cccgtcacag
    10141 tcggcgttca gtgcagcttt caggcagcgc agcgccatcg gactgttttg cagcatttcg
    10201 cggcaccagc gaacggtctc tttttccaga tccgccagcg gtaccacggt gttgacaagg
    10261 cccatatcca gcgcctgttt cgcgtcgtac tgacggcaca ggaaccagat ttcccgcgct
    10321 tttttctgtc cgacgatgcg agccatgtag gaagcgcccc agccgccgtc gaaggaaccg
    10381 actttcgggc cagtctgacc gaagatggca ttatctgccg cgatagtcag gtcgcacatc
    10441 atgtgcagaa cgtgaccgcc gccgatggag tagccagcca ccatcgcgac aaccggtttc
    10501 ggacaggtac ggatctgacg ctggaagtcc agcacgttca ggtgatgtac gccggaatca
    10561 tctttatagc cgccgtaatc accacgcact ttctggtcac cgccggagca gaacgcttta
    10621 tcacctgcgc cagtcagaat gatcacgccg acgttgtcgt cataacgcgc atctgccagt
    10681 gcctggatca tctcttttac cgtcagagga cggaaggcat tgcgcacctg cggacgatta
    10741 atggtgattt ttgcgatacc atcggtggat ttttcataac gaatgtcctc gaaaccttcg
    10801 gagcagtcgt gccattcaac cggtgcgtaa agcattgctt catcaggata aatcatattg
    10861 tgtcctttag tcagaaacgc aagatctgcg ccagacttgc gattacgcca gcgggatttt
    10921 cccgatgcgc gttatgtccg gcgcgaggaa tgacatggca gtcggcagcc agttccgccg
    10981 ccagggcgcg gaatttgctg tcacgttcac cacataaata ataaaacgca aatgtgcggg
    11041 cgctaaggtt agcacgtaaa tcaggctgga cggcgagaga agtcgcctcc agcatggcgg
    11101 caagggttgc gccattattg ttgctgcgca gcgccaccag ctcccggcgt tgatcgtcat
    11161 tgagcgaggc aaaaacaggc tgttgatacc agtcggcaaa taccgccgtt aacggttctg
    11221 tgcgaaaacg ctgcgcccat tggcgatcgg aacgctgacg ttccgcacgt tgttcagcat
    11281 tttgcagccc cggatgcccg ccttcgacaa taaccccaca aagccccgcc agcccctggc
    11341 aagccgccat catcgccacc cgtccgccaa gcgagtaccc caccagccag aagtcaagga
    11401 tgttgtaact aaccaaggtt ttacgcagta agtcggtgac atcatcaaat ccatcgacgc
    11461 taatcgccgc cgaaccacca tgacctggga gatcaacata caaccgtgag tagtcggcaa
    11521 acgcctcgcc cacttcttgc cattcgtggc aatcgccgga aaaaccgtgc aaaaacacca
    11581 gccagggtaa acctggtttt ccgtgttttg cctgcgcgtg caggatcata aatggcttac
    11641 ctgcgccaga agttgctgga gcgtttgcgc accatcggtg tcgttaacca ccatttcaat
    11701 caccgtggtg gttggcgtgc gccaggcgtc ggcaaatgcc gtttcaagtt cctgccagtt
    11761 ttgcggacga tgatatttca gctcgaacat cgcggcggcg tgctcaaaat ggacgttttg
    11821 cggcatcaga tagaaacgct cacgctcgct ttgcggcgtt ggcaacagcg agaaaatttg
    11881 cccgccgttg ttgttcacca caattaatac cagcggcgca gaaacctgac gcaataacgc
    11941 cagcgcgttg agatcgtaaa gtgcggagag atcgcccaca atcgccagcg tcggtttgcc
    12001 gcttgcccgc tgaacgcctg cggcggtcga aagcagtccg tcgataccgc tggcccctcg
    12061 gttgctgtac accgggtaac ctgccggaag ttgcgaaagc gcatcaatca gacgtaccac
    12121 caggctgtta ccgacaaaca attgcccctg ttcaggcaga tagtcgctga tgcgatgcgc
    12181 cagttgcgct tcgccaaacg catcgcgacg ggcaataacc gcctgcattg cctgttccgc
    12241 caggcgcggg atttcaacgc accagggctg gcgtttttct gccggatgca gctccagcca
    12301 gtcggcaata ttggcaatta agcggcgtcc acgatggtgc gccggatcaa gccgcccttc
    12361 gatgtcatcc acaatccagt actcttcggg ttcacagctt gcctgccatt gcaggagccg
    12421 tttgcccgtc aggctgcttc ccagttgcac cacaatttgc gcctgctgca gctcgctggt
    12481 cgctttggca ttgcctaacc aaagatcggc acacggcagc ggctgcccgg tttgtgacag
    12541 cacatcgcca atcagcggcc agccaagagt ttgcgcccac agggcaactt ttttgccctc
    12601 ttccgcactc atgcgcccgg caaccaccac gccgcgcttt tgtcgccaga agaaccagtc
    12661 gcgctgtttt tcactttcca gactaggcgc ttcacgcagc cacggtttgt cgtcctgcca
    12721 ccagtcaccc agacgctgtt gccagctaag cccggtatcg tccatttcgc catacagcgg
    12781 ttcagcaaac gggcagttga tatggactcc ccccgcatga agcgtaccga gagcgtggtc
    12841 gatggtagaa accagccaac gtgcggggat atcctgggtc gggcgcggca atgaaatact
    12901 gtgcgtgggg tgagaggcga acattcccgg ctggcgaatt gcctgattcg cgccgcagtc
    12961 aattagctcc ggcgggcgat cggcggttaa gagaatcagt ttttctccgg ttaacccggc
    13021 ttcaatcagt gccggataga gatttgccac cgccgtgccg gaggtcacaa tcaccgccac
    13081 cggctgcttg ctgactttcg ccagccccag cgccagatgc cccaacccac gctcatcgaa
    13141 atgggtgtgg tgaatgaatg cggaattctc cgccgccgct aacgttaacg gtgtagaacg
    13201 cgagcctggg gcgatacaga tgtgtctgac gccgtgacgc gttaatgctt ccagaatgac
    13261 cgccgcccag cgtcggttaa atgcgcttac tgacatgagt ttgtccggta tcaatattgc
    13321 ggctaagtat aaggagctac aaaaatagat tattgatatg aatcggtaat gatgcgactc
    13381 attactattc catttgtaat aaagtacgca accctgccgc tttgttgtcg atttcctgcc
    13441 actcttgctc ggggtcggaa ccacggacaa tgcccgcgcc agcatataat cgcacgacat
    13501 tgccgctaat ttttgctgag cgcagggaaa cgcagaattc gctttgttgt aatgagagat
    13561 agcccgcaga accggcgtac cattctcggg tgaacggttc gtgacgggcg ataaactgtc
    13621 gcgccagatc gcgcggtaag ccagcaactg ctgccgttgg ctgcaactga tgtaaacaga
    13681 tcacatcatc cgctttgttg agtgaagtcc agatacagcg gcgaagatgc tgcactttac
    13741 gcagacgcag tacctgcggc ggtaaaacat ccagcgtctg ggtatcggcc tgtaatcgtt
    13801 ggcagatatc ttccaccacc agcatgttct cgcgctggtt tttatcatcc gccatcagcc
    13861 actcttctaa ctgctgcgcc tgcttatcat caggatgatt tgctactgtt cccgccagcg
    13921 cttcagtacg cagcgctttg tcacgccgcc gccataaccg ttccggtgaa gagccaagaa
    13981 aagcattttc gccatcaaag gccatgtaaa aatggtagca attcagattc agtcgacgac
    14041 tggcagccat catcgccgcc gcgttgaccg gacttgcgaa atgcaggtca gttgcccgag
    14101 cgagcaccac tttgtcgagc tcaccttcgg cgatggtttt cgttgccagt tcgattaatt
    14161 gcgtccagcc cgttttgtcc ggccagtgtt gttctcgcgt ggtggttaaa tgtaacccag
    14221 gcaagggctt gatactcacc agtgtggcga taaattcttt tgcctgaatc gcatcgtgct
    14281 gaagggagct ttcgctgaat agcgtcagcc gcagcgtggc tttaccgcca cagcgtcgcc
    14341 attccaggcg gggtaaaagt aaattgccct gcgacgggtc aaaagcattc agcccccaaa
    14401 tgcgtaagtc ggcgtgttcc gggtgctggc gaagaaaacg ttgtgcctgg tccaacgacg
    14461 taaaacgggt aatcgcgccc aggacggcag cttcttcatc accattacgt tgttgccagt
    14521 agaattgcgg gtacgtttgc tgactggcca gccagctcaa ggcatcaaaa gcgtctttga
    14581 gagggaaagg aatatcgata acccgaatgc cgggtgtcgc cggaatctct tgcgacaaat
    14641 ggcgcagtag attttccagc gccgtagtaa gtgattgcac gcggacctct ccctgttaaa
    14701 agcctcacat tatacggggt actacaaaaa aaatgcagta ccccggtgta gggaggtttt
    14761 aacggcgtgc cagcaacagt cctagtacca gcccaacggc cgcgcccaca ccaattcctt
    14821 gccaaggttt ttcgtggacg tagtcatcag cacgataaac agcctgcttc gcccgatagt
    14881 aataactgtc tgaaacctgg ctaacccgtt ttttcacatc atccagcgct ttttctgcac
    14941 gcgctttcag ctcaacatat ttctgatcag cgggatcgcc agaggagcgg agcacctctt
    15001 ccagtgtctc actaagcagc gtcaggtcgt catcgatacg tgtatcacca aactgattag
    15061 acattctcgt tctccatttg cgtaaaacct gtcactaact atagacaagg gttgttgatt
    15121 acgcctgaat tacctcgcgt gccatcccaa tgtgtgggat accatcttct tcatagacct
    15181 ccgtcacagg gataaaaccg aaactctggt agaagttttg cagatgcgcc tgcgccccga
    15241 ggtaaacagg cttatcaggc cagtgatgcg tgcaggtgtc gagggtttta ctcatcagtt
    15301 gctgccccac tttttcgcca cgcaacgctt cactgacaat cacccgacca ataacgaccg
    15361 gctcaagatc atcatcactt ttcagaatcc tcgcatatgc caccagttca tcatttttcc
    15421 aaccgagaat atggcgatta tcccccgtca aatcatcacc atcgatatcc tgataagggc
    15481 aattctgctc gacaacaaac accgcgcaac gcagttgtaa taaggcataa agctgggaaa
    15541 ccgataattc agagtgatgc agatcttgcc attcaatcat aatgctctcc ttttagatgt
    15601 ataccttata ctagccggaa tattttttga aacgtgatga actcatggaa ttaatttttt
    15661 taggtacttc agccggtgtg ccaacccgca cgcgcaatgt cacggcaata ttgcttaacc
    15721 tgcaacatcc gacccagagc ggactttggt tgtttgactg cggtgaaggc acccagcatc
    15781 agctactgca taccgccttt aaccctggaa aactggacaa gatttttatc agtcaccttc
    15841 atggcgatca tctttttggt ttacccggct tgctgtgcag tcgttctatg tcaggcatta
    15901 tccaaccctt aacgatttat ggtccgcaag gtatccgtga atttgtggaa accgcgctgc
    15961 ggattagcgg ctcatggacc gattatccgc tggaaattgt cgaaattggc gctggcgaaa
    16021 ttctcgatga tggcctgcgc aaagtaaccg cttatccgct ggaacaccca ctggaatgtt
    16081 atggctatcg tattgaagaa catgataaac cgggtgcatt aaatgcccag gcattaaaag
    16141 ctgctggcgt gccgcctggc ccactgtttc aggaattaaa agcgggcaaa acaatcacgc
    16201 tggaagatgg aaggcagatt aacggcgcag attacttagc tgctccagtg ccaggtaaag
    16261 cgctcgctat tttcggcgat accggcccct gcgatgccgc acttgacctg gctaaaggtg
    16321 tcgatgtcat ggtgcacgaa gcgacgctgg atataaccat ggaagccaaa gccaatagtc
    16381 gcggccatag ctctacacgc caggctgcga cactagcccg tgaggctgga gtcggtaagc
    16441 taatcattac ccacgtcagc tcgcgctatg atgacaaagg ttgtcagcac ctgttacgtg
    16501 aatgcaggtc aattttcccg gcgactgaac tagcgaatga tttcaccgtg tttaacgttt
    16561 aactct
//
LOCUS       DAJTMC010000087        16535 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.087, whole genome shotgun sequence.
ACCESSION   DAJTMC010000087 DAJTMC010000000
VERSION     DAJTMC010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16535
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.087"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(181..1464)
                     /gene="gltA"
                     /locus_tag="OO130_002506"
     CDS             complement(181..1464)
                     /gene="gltA"
                     /locus_tag="OO130_002506"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HCR5365869.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            complement(1670..1777)
                     /locus_tag="OO130_002507"
     CDS             complement(1670..1777)
                     /locus_tag="OO130_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5365870.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            2158..2562
                     /gene="sdhC"
                     /locus_tag="OO130_002508"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             2158..2562
                     /gene="sdhC"
                     /locus_tag="OO130_002508"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCR5365871.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            2556..2903
                     /gene="sdhD"
                     /locus_tag="OO130_002509"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             2556..2903
                     /gene="sdhD"
                     /locus_tag="OO130_002509"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HCR5365872.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            2903..4669
                     /gene="sdhA"
                     /locus_tag="OO130_002510"
     CDS             2903..4669
                     /gene="sdhA"
                     /locus_tag="OO130_002510"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HCR5365873.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            4685..5401
                     /gene="sdhB"
                     /locus_tag="OO130_002511"
     CDS             4685..5401
                     /gene="sdhB"
                     /locus_tag="OO130_002511"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HCR5365874.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            5702..8503
                     /gene="sucA"
                     /locus_tag="OO130_002512"
     CDS             5702..8503
                     /gene="sucA"
                     /locus_tag="OO130_002512"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HCR5365875.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            8518..9735
                     /gene="odhB"
                     /locus_tag="OO130_002513"
     CDS             8518..9735
                     /gene="odhB"
                     /locus_tag="OO130_002513"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HCR5365876.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKSPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            10011..11177
                     /gene="sucC"
                     /locus_tag="OO130_002514"
     CDS             10011..11177
                     /gene="sucC"
                     /locus_tag="OO130_002514"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HCR5365877.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPQLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQLDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKVVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            11177..12046
                     /gene="sucD"
                     /locus_tag="OO130_002515"
     CDS             11177..12046
                     /gene="sucD"
                     /locus_tag="OO130_002515"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HCR5365878.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(12150..12872)
                     /gene="mngR"
                     /locus_tag="OO130_002516"
     CDS             complement(12150..12872)
                     /gene="mngR"
                     /locus_tag="OO130_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HCR5365879.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITAQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            13041..14957
                     /gene="mngA"
                     /locus_tag="OO130_002517"
     CDS             13041..14957
                     /gene="mngA"
                     /locus_tag="OO130_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HCR5365880.1"
                     /translation="MNLTTLTHRDALRLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LEEVYRRESLGPTALGEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPPNEAGTMHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVPACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQALTLKRSDETRTVQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLMRWVKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAIFGFMCSFDLGGPVNKAAYAFCLGAMVNGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            14975..>16535
                     /locus_tag="OO130_002518"
     CDS             14975..>16535
                     /locus_tag="OO130_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HCR5365881.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRVKKQVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITRTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVLPLGYAIGKYLPTDENGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMDKLREIYPQRKFVMSRFEEVFEKIEAQRESLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKIAHARIENKIVNLLEPLATLAWTLGFEYHHGLLEKMWKEILK
                     NHAHDSIGCCCSDKVHREIVARFELAEDMADNLLRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLRDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGSLQLV
                     DKDSGVRYDRVL"
BASE COUNT         3974 a         4147 c         4496 g         3918 t
ORIGIN      
        1 gcaagcacct taaaatcacc atcatacact aaatcagtaa gttggcagca ttaccttttt
       61 tagaatatat gaatgtccga tagtggattt tatttttaca attttttgtg tttaggcata
      121 taaaaattaa cccgccattt gaacggcggg ttaaaatatt tacaacttag caatcaacca
      181 ttaacgcttg atatcgcttt taaagtcgcg tttttcatat cctgtataca gctgacgcgg
      241 acgggcaatc ttcataccgt cactgtgcat ttcgctccag tgggcgatcc agccaacggt
      301 acgtgccatt gcgaaaatga cggtgaacat ggaagacgga atacccatcg ctttcaggat
      361 gataccagag tagaaatcga cgttcgggta cagtttcttc tcgataaagt acgggtcgtt
      421 cagcgcgatg ttttccagct ccatagccac ttccagcagg tcatctttgg tgcccagctc
      481 tttcagaact tcatggcagg tttcacgcat tacggtggcg cgcgggtcgt aatttttgta
      541 cacgcggtga ccgaagccca tcaggcggaa agaatcattt ttatctttcg cacgacgaac
      601 aaattccgga atgtgtttaa cggagctaat ttcttccagc attttcagcg ccgcttcgtt
      661 agcaccaccg tgcgcaggtc cccacagtga agcaatacct gctgcgatac aggcaaacgg
      721 gttcgcaccc gaagagccag cggtacgcac ggtggaggta gaggcgttct gttcatggtc
      781 agcgtgcagg atcagaatac ggtccatagc acgttccaga atcggattaa cttcatacgg
      841 ttcgcacggc gtggagaaca tcatattcag gaagttaccg gcataggaga gatcgttgcg
      901 cggataaaca aatggctgac caatggaata cttgtaacac atcgcggcca tggtcggcat
      961 tttcgacagc aggcggaacg cggcaatttc acgatgacga ggattgttaa catccagcga
     1021 gtcgtgatag aacgccgcca gcgcgccggt aataccacac atgactgcca ttggatgtga
     1081 gtcgcgacgg aaagcgtgga acagacgggt aatctgctcg tggatcatgg tatgacgggt
     1141 caccgtagtt ttaaattcgt catactgttc ctgagtcggt ttttcaccat tcagcaggat
     1201 gtaacaaact tccaggtagt tagaatcggt cgccagctga tcgatcggga aaccgcggtg
     1261 cagcaaaata ccttcatcac catcaataaa agtaatttta gattcgcagg atgcggttga
     1321 agtgaagcct gggtcaaagg tgaacacacc ttttgaaccg agagtacgga tatcaataac
     1381 atcttgaccc agcgtgcctt tcagcacatc cagttcaaca gctgtgtccc cgttgagggt
     1441 gagttttgct tttgtatcag ccatttaagg tctccttagc gccttattgc gtaagactgc
     1501 cggaacttaa atttgccttc gcacatcaac ctggctttac ccgtttgtta tttggctcgc
     1561 cgctctgtga aagaggggaa aacctgggta cagagctctg ggcgcttgca ggtaaaggat
     1621 ccattgatga cgaataaatg gcgaatcaag tacttagcaa tccgaattat taaacttgtc
     1681 taccactaat aactgtcccg aatgaattgg tcaatacttc cacactgtta cataagttaa
     1741 tcttaggtga aataccgact tcataacttt tacgcattat atgcttttcc tggtaatgtt
     1801 tgtaacaact ttgttgagtg attgtcaaat tagatgatta aaaattaaat aaatgttgtt
     1861 gtcgtgacct ggatcactgt tcaggataaa acccgacaaa ctatatgtag gttaattgta
     1921 atgattttgt gaacagccta tactgccgct aggtctccgg aacaccctgc aatcccgagc
     1981 cacccagcgt tgtaacgtgt cgttttcgca tctggaagca gtgttttgca tgacgcgcag
     2041 ttatagaaag gacgctgtct gacccgcaag cagaccggag gaaggaaatc ccgacgtctc
     2101 caggtaacag aaagttaacc tctgtgcccg tagtccccag ggaataataa gaacagcatg
     2161 tgggcgttat tcatgataag aaatgtgaaa aaacaaagac ctgttaatct ggacctacag
     2221 accatccggt tccccatcac ggcgatagcg tccattctcc atcgcgtttc cggtgtgatc
     2281 acctttgttg cagtgggcat cctgctgtgg cttctgggta ccagcctctc ttcccctgaa
     2341 ggtttcgagc aagcttccgc gattatgggc agcttcttcg tcaaatttat catgtggggc
     2401 atccttaccg ctctggcgta tcacgtcgtc gtaggtattc gccacatgat gatggatttt
     2461 ggctatctgg aagaaacatt cgaagcgggt aaacgctccg ccaaaatctc ctttgttatt
     2521 actgtcgtgc tttcacttct cgcaggagtc ctcgtatggt aagcaacgcc tccgcattag
     2581 gacgcaatgg cgtacatgat ttcatcctcg ttcgcgctac cgctatcgtc ctgacgctct
     2641 acatcattta tatggtcggt tttttcgcca ccagtggcga actgacatat gaagtctgga
     2701 tcggtttctt cgcctctgcg ttcaccaaag tgttcaccct gctggcgctg ttttctatct
     2761 tgattcatgc ctggatcggc atgtggcagg tgttgaccga ctacgttaaa ccgctggcat
     2821 tgcgcctgat gctgcaactg gtgattgtcg ttgcactggt ggtttacgtg atttatggat
     2881 tcgttgtggt gtggggtgtg tgatgaaatt gccagtcaga gaatttgatg cagttgtgat
     2941 tggtgccggt ggcgcaggta tgcgcgcggc gctgcaaatt tcccagagcg gccagacctg
     3001 tgcgctgctc tctaaagtct tcccgacccg ttcccatacc gtttctgcgc aaggcggcat
     3061 taccgttgcg ctgggtaata cccatgaaga taactgggaa tggcatatgt acgacaccgt
     3121 aaaagggtcg gactatatcg gtgaccagga cgcgattgaa tatatgtgta aaaccgggcc
     3181 ggaagcgatt ctggaactcg aacacatggg cctgccgttc tcgcgtcttg atgatggtcg
     3241 tatctatcaa cgtccgtttg gcggtcagtc gaaaaacttc ggcggcgagc aggcggcacg
     3301 cactgcggcg gcggctgacc gtaccggtca cgcactgttg cacacgcttt atcagcagaa
     3361 cctgaaaaac cacaccacca ttttctccga gtggtatgcg ctggatctgg tgaagaacca
     3421 ggatggcgcg gtggtgggtt gtactgcact gtgcatcgaa actggtgaag tggtttattt
     3481 caaagcccgc gctaccgtgc tggcgactgg cggggcaggg cgtatttatc agtccaccac
     3541 taacgcccac attaacactg gcgacggcgt cggcatggct atccgcgcag gcgtaccggt
     3601 gcaggatatg gaaatgtggc agttccaccc gaccggtatt gccggtgcgg gcgtactggt
     3661 caccgaaggt tgccgtggtg aaggcggtta tctgctgaac aaacatggcg aacgctttat
     3721 ggagcgttat gcgccgaacg ccaaagacct ggcgggccgt gatgtggtgg cgcgttccat
     3781 catgatcgaa atccgtgaag gtcgcggctg tgatggtccg tgggggccac acgcgaaact
     3841 gaaactcgac cacctgggta aagaagttct cgaatcccgt ctgccgggca tcctggaact
     3901 ctcccgtacc ttcgctcacg tcgatccggt gaaagagccg atcccggtta tcccaacctg
     3961 tcattacatg atgggcggta ttccgaccaa agtgaccggt caggcgctga ctgtgaatga
     4021 gaaaggcgaa gatgtggttg ttccgggact gtttgccgtt ggtgaaatcg cttgtgtatc
     4081 ggtacacggc gctaaccgtc tgggcggcaa ctcgctgctg gacctggtgg tctttggtcg
     4141 tgcggcaggt ctgcatctgc aagagtctat cgccgagcag ggcgcattgc gcgatgccag
     4201 cgagtctgat gttgaagcat ctctggatcg tctgaaccgc tggaacaata atcgtaacgg
     4261 tgaagatccg gtggcgattc gtaaagcgct gcaagaatgt atgcagcata acttctcggt
     4321 cttccgtgaa ggtgatgcga tggcgaaagg gcttgagcag ttgaaagtta tccgcgagcg
     4381 tctgaaaaat gcccgtctgg atgacacttc cagcgagttc aacacccagc gcgttgagtg
     4441 cctggaactg gataacctga tggaaacggc gtatgcaacg gctgtttctg ccaacttccg
     4501 taccgaaagc cgtggcgcgc atagccgctt cgacttcccg gatcgtgatg atgaaaactg
     4561 gctgtgccac tccctgtatc tgccagagtc ggaatccatg acgcgccgaa gcgtcaacat
     4621 ggaaccgaaa ctgcgcccgg cattcccgcc gaagattcgt acttactaat gcggagacag
     4681 gaaaatgaga ctcgagtttt caatttatcg ctataacccg gatgttgatg atgctccgcg
     4741 tatgcaggat tacaccctgg aagcggaaga aggtcgcgac atgatgctgc tggatgcgct
     4801 tatccagctg aaagagaaag atcccagcct gtcgttccgc cgctcctgtc gtgaaggtgt
     4861 gtgcggttcc gacggtctga acatgaacgg caagaatggt ctggcctgta ttaccccgat
     4921 ttcggcactc aaccagccgg gcaagaagat tgtgattcgc ccgctgccag gtttaccggt
     4981 gatccgcgat ttggtggtag acatgggaca attctatgcg caatatgaga aaattaagcc
     5041 ttacctgttg aataatggac aaaatccgcc agctcgcgag catttacaga tgccagagca
     5101 gcgcgaaaaa ctcgacgggc tgtatgaatg tattctctgc gcatgttgtt caacctcttg
     5161 tccgtctttc tggtggaatc ccgataagtt tatcggcccg gcaggcttgt tagcggcata
     5221 tcgtttcctg atcgatagcc gtgataccga gactgacagc cgcctcgacg gtttgagcga
     5281 tgcattcagc gtattccgct gtcacagcat catgaactgc gtcagtgtat gtccgaaggg
     5341 gctgaacccg acgcgcgcca tcggccatat caagtcgatg ttgttgcaac gtaatgcgta
     5401 aaccgtaggc ctgataagac gcgcaagcgt cgcatcaggc aaccagtgcc ggatgcggcg
     5461 tgaacgcctt atccggccta caagtcatta cccgtaggcc tgataagcgc agcgcatcag
     5521 gcgtaacaaa gaaatgcagg aaatctttaa aaactgcccc tgacactaag acagttttta
     5581 aaggttcctt cgcgagccac tacgtagaca agagctcgca agtgaacccc ggcacgcaca
     5641 tcactgtgcg tggtagtatc cacggcgaag taagcataaa aaagatgctt aagggatcac
     5701 gatgcagaac agcgctttga aagcctggtt ggactcttct tacctctctg gcgcaaacca
     5761 gagctggata gaacagctct atgaagactt cttaaccgat cctgactcgg ttgacgctaa
     5821 ctggcgttcg acgttccagc agttacctgg tacgggagtc aaaccggatc aattccactc
     5881 tcaaacgcgt gaatatttcc gccgcctggc gaaagacgct tcacgttact cttcaacgat
     5941 ctccgaccct gacaccaatg tgaagcaggt taaagtcctg cagctcatta acgcataccg
     6001 cttccgtggt caccagcatg cgaatctcga tccgctggga ctgtggcagc aagataaagt
     6061 ggccgatctg gatccgtctt tccacgatct gaccgaagca gacttccagg aaaccttcaa
     6121 cgtcggttca tttgccagcg gcaaagaaac catgaaactc ggcgagctgc tggaagccct
     6181 caagcaaacc tactgcggcc cgattggtgc cgagtatatg cacatcacca gcaccgaaga
     6241 aaaacgctgg atccaacagc gtatcgagtc tggtcgcgcg actttcaata gcgaagagaa
     6301 aaaacgcttc ttaagcgaac tgaccgccgc tgaaggcctt gaacgttacc tcggcgcaaa
     6361 attccctggc gcaaaacgct tctcgctgga aggcggtgac gcgttaatcc cgatgcttaa
     6421 agagatgatc cgccacgctg gcaacagcgg cacccgcgaa gtggttctcg ggatggcgca
     6481 ccgtggtcgt ctgaacgtgc tggtgaacgt gctgggtaaa aaaccgcaag acttgttcga
     6541 cgagttcgcc ggtaaacata aagaacacct cggcacgggt gacgtgaaat accacatggg
     6601 cttctcgtct gacttccaga ccgatggcgg cctggtgcac ctggcgctgg cgtttaaccc
     6661 gtctcacctt gagattgtaa gcccggtagt tatcggttct gttcgtgccc gtctggacag
     6721 acttgatgag ccgagcagca acaaagtgct gccaatcacc atccacggtg acgccgcagt
     6781 gaccgggcag ggcgtggttc aggaaaccct gaacatgtcg aaagcgcgtg gttatgaagt
     6841 tggcggtacg gtacgtatcg ttatcaacaa ccaggttggt ttcaccacct ctaatccgct
     6901 ggatgcccgt tctacgccgt actgtactga tatcggtaag atggttcagg ccccgatttt
     6961 ccacgttaac gcggacgatc cggaagccgt tgcctttgtg acccgtctgg cgctcgattt
     7021 ccgtaacacc tttaaacgtg atgtcttcat cgacctggtg tgctaccgcc gtcacggcca
     7081 caacgaagcc gacgagccga gcgcaaccca gccgctgatg tatcagaaaa tcaaaaaaca
     7141 tccgacaccg cgcaaaatct acgctgacaa gctggagcag gaaaaagtgg cgacgctgga
     7201 agatgccacc gagatggtta acctgtaccg cgatgcgctg gatgctggcg attgcgtagt
     7261 ggcagagtgg cgtccgatga acatgcactc tttcacctgg tcgccgtacc tcaaccacga
     7321 atgggacgaa gagtacccga acaaagttga gatgaagcgc ctgcaggagc tggcgaaacg
     7381 catcagcacg gtgccggaag cagttgaaat gcagtctcgc gttgccaaga tttatggcga
     7441 tcgccaggcg atggctgccg gtgagaaact gttcgactgg ggcggtgcgg aaaacctcgc
     7501 ttacgccacg ctggttgacg aaggcattcc ggttcgcctg tcgggtgaag actccggtcg
     7561 cggtaccttc ttccaccgcc acgcggtgat ccacaaccag tctaacggtt ccacttacac
     7621 gccgctgcaa cacatccata acgggcaggg cgcgttccgc gtctgggact ccgtactgtc
     7681 tgaagaagca gtgctggcgt ttgaatacgg ttatgccacc gcagaaccac gcactctgac
     7741 catctgggaa gcgcagttcg gtgacttcgc taacggtgct caggtggtta tcgaccagtt
     7801 catctcctct ggcgaacaga aatggggccg gatgtgtggt ctggtgatgt tgctgccgca
     7861 cggttatgaa gggcaggggc cggagcactc ctccgcgcgt ctggaacgtt atctgcaact
     7921 ttgtgctgag caaaacatgc aggtgtgcgt accgtctacc ccggcacagg tttaccacat
     7981 gctgcgtcgt caggcgctgc gcgggatgcg tcgtccgctg gtcgtgatgt cgccgaaatc
     8041 cctgctgcgt catccgctgg cggtttccag cctcgaagaa ctggcgaacg gcaccttcct
     8101 gccagccatc ggtgaaatcg acgagcttga tccgaagggc gtgaagcgcg tagtgatgtg
     8161 ttctggtaag gtttattacg acctgctgga acagcgtcgt aagaacaatc aacacgatgt
     8221 cgccattgtg cgtatcgagc aactctaccc gttcccgcat aaagcgatgc aggaagtgtt
     8281 gcagcagttt gctcacgtca aggattttgt ctggtgccag gaagagccgc tcaaccaggg
     8341 cgcatggtac tgcagccagc atcatttccg tgaagtgatt ccgtttgggg cttctctgcg
     8401 ttatgcaggc cgcccggcct ccgcctctcc ggcggtaggg tatatgtccg ttcaccagaa
     8461 acagcaacaa gatctggtta atgacgcgct gaacgtcgaa taaataaagg atacacaatg
     8521 agtagcgtag atattctggt ccctgacctg cctgaatccg tagccgatgc caccgtcgca
     8581 acctggcata aaaaacccgg cgacgcagtc gtacgtgatg aagtgctggt agaaatcgaa
     8641 actgacaaag tggtactgga agtaccggca tcagcagacg gcattctgga tgcggttctg
     8701 gaagatgaag gtacaaccgt aacgtctcgt cagatccttg gtcgcctgcg tgaaggcaac
     8761 agcgccggta aagaaaccag cgccaaatct gaagagaaag cgtccactcc ggcgcaacgc
     8821 cagcaggcgt ctctggaaga gcaaaacaac gatgcgttaa gcccggcgat ccgtcgcttg
     8881 ctggctgaac ataacctcga cgccagcgct attaaaggca ccggcgtggg tggtcgtctg
     8941 acccgtgaag atgtggaaaa acatctggcg aaatccccgg cgaaagagtc tgcaccggca
     9001 gcggctgctc cggcggcgca accggctctg gctgcacgta gtgaaaaacg tgtcccgatg
     9061 actcgcctgc gtaagcgtgt ggcagagcgt ctgctggaag cgaaaaactc caccgccatg
     9121 ctgaccacgt tcaacgaagt caacatgaag ccgattatgg atctgcgtaa gcagtacggt
     9181 gaagcgtttg aaaaacgcca cggcatccgt ctgggcttta tgtccttcta cgtgaaagcg
     9241 gtggttgaag ccctgaaacg ttacccggaa gtgaatgctt ctatcgacgg cgatgacgtg
     9301 gtttaccaca actatttcga cgtcagcatg gcggtttcta cgccgcgcgg cctagtgacg
     9361 ccggttctgc gtgatgtcga taccctcggc atggcagaca tcgagaagaa aatcaaagag
     9421 ctggcagtca aaggccgtga tggcaagctg accgttgaag atctgaccgg tggtaacttc
     9481 accatcacca acggtggtgt gttcggttcc ctgatgtcta cgccgatcat caacccgccg
     9541 cagagcgcaa ttctgggtat gcacgctatc aaagatcgtc cgatggcggt gaatggtcag
     9601 gttgagatcc tgccgatgat gtacctggcg ctgtcctacg atcaccgtct gatcgatggt
     9661 cgcgaatccg tgggcttcct ggtaacgatc aaagagttgc tggaagatcc gacgcgtctg
     9721 ctgctggacg tgtagtagtt taagtttcac ctgcactgta gacaggataa ggcattatcg
     9781 ccttctccgg caattgaagc ctgatgcgac gcttaacgcg tcttatcagg cctacgggaa
     9841 gcaccaatgt aggtcggata aggcgcttgc gccgcatccg acaagcgatg cctgatgcga
     9901 cacttacgcg tcttatcagg cctacaggtg accgacaatg cccggaggcg atacgaaata
     9961 ttcggtctac ggtttaaaag ataacgattg ctgaaggatg gacagaacac atgaacttac
    10021 atgaatatca ggcaaaacaa ctttttgccc gctatggctt accagcaccg gtgggttatg
    10081 cctgtactac tccgcgcgaa gcagaagaag ccgcttcaaa aatcggtgcc ggtccgtggg
    10141 tagtgaaatg tcaggttcac gctggtggcc gcggtaaagc gggcggtgtg aaagttgtaa
    10201 acagcaaaga agacatccgt gcttttgcag aaaattggct cggcaagcgt ctggtaacgt
    10261 atcaaacaga tgccaatggc caaccggtta accagattct ggttgaagca gcgaccgata
    10321 tcgctaaaga gctgtatctc ggtgccgttg tagaccgtag ttcccgtcgt gtggtcttta
    10381 tggcctccac tgaaggcggc gtggaaatcg aaaaagtggc ggaagaaact ccgcagctga
    10441 tccataaagt tgcgcttgat ccgctgacag gcccgatgcc gtatcaggga cgcgagctgg
    10501 cgttcaaact gggtctggaa ggtaaactgg ttcagcagtt caccaaaatc ttcatgggtc
    10561 tggcgaccat tttcctggag cgcgacctgg cgttgatcga aatcaacccg ctggtcatca
    10621 ccaaacaggg tgatctgatt tgcctcgacg gcaaactggg cgctgatggc aacgcactgt
    10681 tccgccagct tgatctgcgc gaaatgcgtg accagtcgca ggaagatccg cgtgaagcac
    10741 aggctgcaca gtgggaactg aactacgttg cgctggacgg taacatcggt tgtatggtta
    10801 acggcgcagg tctggcgatg ggtacgatgg acatcgttaa actgcacggc ggcgaaccgg
    10861 ctaacttcct tgacgttggc ggcggtgcaa ccaaagaacg tgtaaccgaa gcgttcaaaa
    10921 tcatcctctc tgacgacaaa gtgaaagtcg ttctggttaa catcttcggc ggtatcgttc
    10981 gttgcgacct gatcgctgac ggtatcatcg gcgcggtagc agaagtgggt gttaacgtac
    11041 cggtcgtggt acgtctggaa ggtaacaacg ccgaactcgg cgcgaagaaa ctggctgaca
    11101 gcggcctgaa tattattgca gcaaaaggtc tgacggatgc agctcagcag gttgttgccg
    11161 cagtggaggg gaaataatgt ccattttaat tgataaaaac accaaggtta tctgccaggg
    11221 ctttactggt agccagggga ctttccactc agaacaggcc attgcatacg gcactaaaat
    11281 ggttggcggc gtaaccccag gtaaaggcgg tactacccac ctcggcctgc cggtgttcaa
    11341 caccgtgcgt gaagctgttg ctgctactgg cgctaccgct tctgttatct acgtaccagc
    11401 accgttctgc aaagactcca ttctggaagc tattgatgca ggcatcaaac tgattatcac
    11461 catcactgaa ggcatcccga cgctggatat gctgactgtg aaagtgaaac tggacgaagc
    11521 cggcgttcgt atgatcggcc cgaactgccc gggcgttatc actccgggtg aatgcaaaat
    11581 cggtatccag ccgggtcaca ttcacaaacc gggtaaagtg ggtatcgttt cccgttccgg
    11641 tacactgacc tatgaagcgg ttaaacagac cacggattac ggtttcggtc agtcgacctg
    11701 tgttggtatc ggcggtgacc cgatcccagg ctctaacttt atcgacatcc tcgaaatgtt
    11761 cgaaaaagat ccgcagaccg aagcgatcgt gatgatcggt gagatcggcg gtagcgctga
    11821 agaagaagcg gcagcgtaca tcaaagagca cgttaccaag ccagttgtag gttacatcgc
    11881 tggtgtgact gcgccgaaag gcaaacgtat gggccacgcg ggtgcgatca ttgccggtgg
    11941 gaaagggact gcggatgaga aattcgctgc tctggaagcc gcaggcgtga aaaccgttcg
    12001 cagcctggcg gatatcggtg aagcactgaa aactgttctg aaataaatat ctgtaataag
    12061 aaatagccct cgccgcttcc ctctacagga atggcgaagg gctgtcggtt tcgacatggt
    12121 tggccatcgt atgatggcct tttttgtgct tatcgcgatg attttcgctg cgctatcagg
    12181 gtaaatttat agtcatcggt attaaaagcg ttgcggctat attcaaacac ccgaccatca
    12241 actaaatatc cacgcgatac tttttcaaga atcggctttg tctggctgat attaagcaga
    12301 cggctcatct cttcggttgg catcagagga atgatttcct gttcgctacg atcgataacc
    12361 atcttcttca cttcttcaat aaagtgatat ttcgaatttt ccatgacctg ccaggtgaga
    12421 tccgggaaca acgcaagcgg catccaggtt tcttccagcg ccattggctt ttgcttgcga
    12481 tagcgcacgc gcttcacatg ccacacacga tcctgcgcgg tgatttgtag ctgttgctga
    12541 agaaaatcgt cagccggaat cacttcaaat atcaggactt cactgtgtgt atcgacgtga
    12601 cggtccgaca gtttttcatc aaaactggtt aactgaaaaa tatcgtaatt gacccgctct
    12661 tctttgacgt aagtcccgct gccctgaatg ctttcgagga tctgctgctc gactagctgg
    12721 cgcaaagcct gacgcaccgt aacccggctg acgccaaact ctgtttgtag cgctgattca
    12781 gtgggtaacg catcgccagg tttaagctcg ccacgcgcaa tttgttcacg aatgcgatcg
    12841 gcaatctgcc ggtataaggg cttgtgtccc atttttagta tctcattaat acgaatttag
    12901 ccattatgcc cgataaattc atcctgtaaa taatgcaaat acaatacaaa taatttcaat
    12961 caagtgaaat tgatcatata atggtattgt tttatcgggc acactggcgc gactataaaa
    13021 acgatcaagt gaggatcatg atgaatctga cgactctgac ccaccgcgat gcgttgcgtc
    13081 tgaatgcgcg ctttaccagc cgtgaagagg ccatccacgc gttgactcaa cgtcttgctg
    13141 ctctggggaa aatttccagt actgagcaat ttctggaaga agtgtatcgc cgtgaaagcc
    13201 ttggcccgac cgccttaggt gaagggttgg ctgtgccgca tggcaaaact gctgcggtaa
    13261 aagaagcggc gtttgcggtc gccacactca gcgagccgct tcagtgggaa ggcgttgatg
    13321 gcccggaagc agttgattta gtggtgctgc tggcgattcc ccccaatgaa gcgggtacta
    13381 tgcatatgca actgctgaca gcgctgacca cgcgccttgc ggatgatgag attcgggcgc
    13441 gtatacagtc ggcgacgacg cctgatgagt tgctctcggc gctggatgac aagggaggca
    13501 cgcaaccttc tgcctctttt tccaacgcgc caactatcgt ctgcgtaccg gcctgtccgg
    13561 cgggtattgc tcacacctat atggctgcgg aatatctgga aaaagccgga cgcaaactcg
    13621 gcgtaaatgt ttacgttgaa aaacaaggcg ctaacggcat tgaagggcgt ttaacggcgg
    13681 atcaactcaa tagtgcaacc gcctgtattt ttgcggctga agtcgccatc aaggagagtg
    13741 agcgttttaa tggcattccc gcgctttcag tgcctgttgc cgagccgatt cgccatgcag
    13801 aagcgttgat ccaacaagcg cttaccctca agcgtagcga tgagacgcgt accgtacagc
    13861 aagatacgca accggtgaaa agtgtcaaaa cggagctgaa acaggcactg ttgagcggaa
    13921 tctcttttgc cgtaccgttg attgtcgcgg ggggcacggt gctggcggtc gcggtattac
    13981 tgtcgcaaat cttcgggcta caagatctgt ttaatgaaga aaactcctgg ctgtggatgt
    14041 accgcaagct gggcggcggg ctgctcggaa ttttgatggt accggtgctc gcggcctata
    14101 ctgcctattc tctggcagat aaaccggcgt tagcgccagg ctttgcggct ggacttgccg
    14161 ccaacatgat cggctccggg tttctcggcg cggtcgttgg cggattgata gccggttact
    14221 tgatgcgctg ggtgaaaaat cacttgcgtc ttagcagtaa attcaatgga ttcctgactt
    14281 tttatctcta cccggtgctc ggtacgttgg gagcgggcag tctgatgctg tttgtggtgg
    14341 gagaacctgt cgcatggatc aataactcgc ttaccgcctg gctgaacggt ctgtcaggaa
    14401 gtaacgcgct gttgctgggt gccattttcg gttttatgtg ttcctttgat cttggagggc
    14461 cagtgaacaa agccgcttat gcattctgcc tgggcgcaat ggtgaacggc gtttacggcc
    14521 cgtatgccat tttcgcctcc gtcaaaatgg tttcggcatt taccgtaacc gcttccacga
    14581 tgctcgcacc gcgcctgttt aaagagtttg aaattgagac cgggaaatcc acctggctgt
    14641 tagggctggc aggtattacc gaaggggcga tcccgatggc gattgaagat ccgctgcggg
    14701 ttattggttc gtttgtgctg ggctctatgg taacgggcgc tattgtcggt gcgatgaata
    14761 tcggcctttc gacacccggt gccggcattt tctcgctctt tttacttcat gataatggcg
    14821 cgggcggtgt tatggcggca attggctggt ttggtgcggc attggtgggg gctgcaatct
    14881 cgactgcaat tctcctgatc tggcggcgtc acgcggttaa gcatggcaac tatctgactg
    14941 atggcgtaat gccataaaca aaaaggaaac gacgatgaaa gcggtatctc gtgttcacat
    15001 caccccgcat atgcactggg atcgagagtg gtatttcacc accgaagagt cacgtattct
    15061 gctggtcaat aatatggaag agatcctgtg ccgactggaa caggacaacg aatacaaata
    15121 ttacgtactc gacgggcaaa cggcgatcct cgaagattat ttcgcggtga aaccggaaaa
    15181 caaagaccgt gtgaagaaac aggtagaagc cggcaagttg attatcggcc cctggtatac
    15241 ccagaccgat accacgattg tttctgcgga atccatcgtc cgtaatctga tgtacggaat
    15301 gcgtgactgc ctcgcgtttg gcgagccgat gaaaataggt tatttaccag attcctttgg
    15361 catgtccggg caactgccgc atatctacaa tggatttggc attacccgca ccatgttctg
    15421 gcgcggatgt tcggagcgcc acggtactga taaaaccgag tttttgtggc aaagcagtga
    15481 cggtagcgaa gtgacggcgc aggtgctgcc gctgggctac gccataggta agtacttacc
    15541 taccgacgaa aacggattac gtaaacgcct cgacagttat tttgacgtgc tggaaaaagc
    15601 gtctgtaacc aaagagattt tgctgccgaa tggtcatgac cagatgccat tgcagcaaaa
    15661 tatcttcgaa gtgatggata agctacgtga gatctaccct caacgtaagt ttgtgatgag
    15721 ccgctttgaa gaggtatttg agaagatcga agcgcagcgt gaaagtctgg ccaccctgaa
    15781 aggtgaattt atcgatggca aatatatgcg cgtacatcgc accatcggtt ccacgcgcat
    15841 ggatatcaaa attgcccacg cacgtattga aaataagatt gttaatctgc tggaaccgct
    15901 ggcaacgctg gcctggacgt tgggttttga ataccaccac ggcttgctgg agaaaatgtg
    15961 gaaagagatc ttaaaaaatc atgcccacga cagtatcggt tgttgttgca gtgacaaagt
    16021 tcatcgcgaa attgtcgccc gcttcgaact ggctgaagac atggcggata atctgctgcg
    16081 tttctacatg cgcaaaattg ccgataacat gccacagagt gatgccgaca aactcgtgct
    16141 gtttaacctg atgccctggc cgcgggaaga agttattaac accactgtgc ggctgcgcgc
    16201 cagccagttt aatctacgag acgatcgcgg tcagcctgta ccgtatttta ttcgccatgc
    16261 ccgtgagatc gatccaggcc taatcgatcg gcaaatagtt cattacggta attacgatcc
    16321 ctttatggag tttgatatac agatcaacca gattgtccct tctatgggct atcgcacgct
    16381 ttatatcgaa gcgaatcagc ctggcaacgt gattgcggca aaaagtgacg ctgaagggat
    16441 actggaaaat gctttctggc aaattgcgct caatgaggat ggttctctgc aactggtaga
    16501 taaagacagc ggtgtgcgct atgaccgagt attgc
//
LOCUS       DAJTMC010000088        16519 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.088, whole genome shotgun sequence.
ACCESSION   DAJTMC010000088 DAJTMC010000000
VERSION     DAJTMC010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16519
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.088"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            93..2321
                     /gene="crfC"
                     /locus_tag="OO130_002519"
     CDS             93..2321
                     /gene="crfC"
                     /locus_tag="OO130_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HCR5365882.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAAQLAL
                     PWSQPNIAERYAMLNNELRKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARYELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDALNAQLAALFQD
                     DSEPLSEIRTCCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHVNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHGWIYTDAQLLRDDIQTLFTAE
                     RY"
     gene            2318..3201
                     /locus_tag="OO130_002520"
                     /pseudo
     CDS             2318..3201
                     /locus_tag="OO130_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
     gene            3465..4967
                     /gene="proP"
                     /locus_tag="OO130_002521"
     CDS             3465..4967
                     /gene="proP"
                     /locus_tag="OO130_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HCR5365883.1"
                     /translation="MLKRKKVKPITLRDVTIINDSKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            5033..5170
                     /gene="pmrR"
                     /locus_tag="OO130_002522"
     CDS             5033..5170
                     /gene="pmrR"
                     /locus_tag="OO130_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HCR5365884.1"
                     /translation="MSDAHTLLQVNGGECNEKPCLYESLTTVFSVLVVSSFLYIWFAT
                     F"
     gene            complement(5146..6246)
                     /gene="pmrB"
                     /locus_tag="OO130_002523"
     CDS             complement(5146..6246)
                     /gene="pmrB"
                     /locus_tag="OO130_002523"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HCR5365885.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(6247..6915)
                     /gene="basR"
                     /locus_tag="OO130_002524"
     CDS             complement(6247..6915)
                     /gene="basR"
                     /locus_tag="OO130_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HCR5365886.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKSRIRTVRGFGYMLVA
                     NEEN"
     gene            complement(6912..8555)
                     /gene="eptA"
                     /locus_tag="OO130_002525"
     CDS             complement(6912..8555)
                     /gene="eptA"
                     /locus_tag="OO130_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HCR5365887.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWIKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTV
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPGQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSK"
     gene            complement(8659..9996)
                     /gene="adiC"
                     /locus_tag="OO130_002526"
     CDS             complement(8659..9996)
                     /gene="adiC"
                     /locus_tag="OO130_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HCR5365888.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTPGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            complement(10133..10894)
                     /gene="adiY"
                     /locus_tag="OO130_002527"
     CDS             complement(10133..10894)
                     /gene="adiY"
                     /locus_tag="OO130_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HCR5365889.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            complement(11219..13486)
                     /gene="adiA"
                     /locus_tag="OO130_002528"
     CDS             complement(11219..13486)
                     /gene="adiA"
                     /locus_tag="OO130_002528"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HCR5365890.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTTDFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     YWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHAMCVKA"
     gene            complement(13685..14593)
                     /gene="melR"
                     /locus_tag="OO130_002529"
     CDS             complement(13685..14593)
                     /gene="melR"
                     /locus_tag="OO130_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HCR5365891.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMMIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            14876..16231
                     /gene="melA"
                     /locus_tag="OO130_002530"
     CDS             14876..16231
                     /gene="melA"
                     /locus_tag="OO130_002530"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HCR5365892.1"
                     /translation="MMTAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALQDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATLLNYVNPMAMNTWAM
                     YARYPHIKQVGLCHSVQGTAEELARDLNIDPSTLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPHIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGESSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            16334..>16519
                     /locus_tag="OO130_002531"
     CDS             16334..>16519
                     /locus_tag="OO130_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5365893.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIM"
BASE COUNT         3986 a         4001 c         4348 g         4184 t
ORIGIN      
        1 tttcttgcag taattataaa agccgaaaac agaactcaaa aacaatctgg ctaaataaaa
       61 gtaacaaaat ttgctttaag gaagaatttt ctatgtacac acagaccctg tatgagttaa
      121 gtcaggaggc tgaacgcctg ttacagcttt ctcgccaaca gttgcagtta ctggaaaaaa
      181 tgcctctctc tgtacccgga gacgatgcgg cacaactggc tttaccctgg agtcagccta
      241 atatcgccga acgttacgcg atgctgaata atgagttgcg taaaatttcc cgactggaaa
      301 tggtgctggc gattgtcggt actatgaaag cagggaaatc aaccaccatt aatgccattg
      361 ttggtacgga ggttctgcct aatcgtaatc gcccaatgac tgcgctgccg acgcttattc
      421 gccatacgcc cgggcaaaag gaaccggtac tgcatttttc acatgtcgcg ccaatcgatt
      481 gtttaattca acaattacaa cagcgcctgc gtgattgcga tattaagcat ctgaccgatg
      541 tgctggaaat agataaagat atgcgtgcgc ttatgcagcg gatcgaaaat ggcgtcgctt
      601 tcgaaaaata ttatctgggt gcccagccta tttttcattg tctgaaaagt ttgaatgatt
      661 tagtgcgact ggcgaaggcg ctggacgtcg attttccttt ttctgcttac gccgccattg
      721 agcatattcc cgtgattgaa gtggagtttg tccatctggc ggggctggaa agttatcccg
      781 gtcagttgac gttactggat acccccgggc caaatgaagc cgggcaaccg catctgcaaa
      841 aaatgcttaa ccagcagctg gcacgcgcct cggcggtact ggcggtgctg gattatacgc
      901 aactgaaatc gatctccgat gaagaggtcc gtgaggcgat tttggcggtg gggcaatcgg
      961 tgccgctgta tgtgctggtc aataagttcg atcaacagga tcgtaacagt gacgacgccg
     1021 accaggtgcg ggcactgatt tccgggacgc tgatgaaagg ctgtattacg ccacagcaga
     1081 tatttccggt gtcgtcgatg tggggctacc tggcgaatcg ggcgcgctat gagttagcca
     1141 acaacggtaa gttaccagcg ccagagcaac aacgctgggt ggaagatttt gcccatgccg
     1201 cgctcggcag gcgctggcgt catgccgatc tggcggacct cgaacatatt cgtcatgctg
     1261 ccgatcagtt gtgggaagat tcgctgttcg cccagccaat tcaggcgttg cttcatgccg
     1321 cttacgctaa cgcctcattg tatgctctgc gatctgctgc gcataaactg ttgaattacg
     1381 cgcagcaggc gcgggaatac ctggattttc gtgcgcacgg gttaaacgtc gcttgtgaac
     1441 aattgcggca aaatatccat caggtcgaag aaagtttgca gctattgcaa ctcaatcagg
     1501 cgcaggtgag cggcgagatt aaacatgaaa tcgagctggc cctgacctcc gccaaccact
     1561 ttctgcgtca acagcaagat gcgctgaatg cgcagttagc cgccttgttt caggatgatt
     1621 cggagccgtt aagcgagatt cgtacctgct gtgagacact gttacagacg gcgcagaaca
     1681 ccatcagtcg cgactttacg ctgcgttttg ccgagcttga atccaccctt tgccgggtgt
     1741 taaccgatgt tattcggccc attgagcaac aagtcaaaat ggaattgagc gagtcagggt
     1801 ttcgtcctgg gtttcatttt cctgtttttc acggagtagt tccccacgtc aacactcgcc
     1861 agctgttcag tgaagtcatt tcgcgccagg aggcaacgga cgagcagagc acgcgtttag
     1921 gcgttgtgcg tgagactttt tcgcgctggt tgaatcagcc cgactgggga cggggaaatg
     1981 agaaatcccc gacagaaacg gttgattaca gtgtgttgca acgagcatta agcgcagaag
     2041 tcgatcttta ttgccaacaa atggctaaag ttctggcaga gcaggtcgat gaatctgtta
     2101 cggcaggcat gaatactttt ttcgctgagt tcgcttcatg tttgacggaa ttacagacgc
     2161 gtttacgcga aagcctggct ctgcgtcaac aaaatgaatc ggtggtcagg ctgatgcagc
     2221 agcaattgca gcagactgtg atgactcacg gctggattta caccgacgcc cagctgttac
     2281 gcgatgatat tcaaacactt ttcacggcag aacgatattg accaagacgt tactggacgg
     2341 ccccggtcgc gtgctggagt cggtttatcc ccgcttttta gtggatctgg cgcagggcga
     2401 tgatgcccgc cttccacaag cccatcagca gcagtttcgt gaacgactga tgcaggaact
     2461 tctttcgcgt gtgcagcttc agacatggac gaacggcggc atgttaaatg cgccgcttag
     2521 cctgcgactg acaatggtgg aaaaactggc gtcgatgctg gatcccggtc atctggcgct
     2581 gacgcagatc gcgcagcatc tggcgctgct gcaaaaaatg gatcaccgcc agcactctgc
     2641 tttcccggag ctcccccagc aaattgccgc cttgtatgag tggttttcag cccgttgtcg
     2701 ctggaaggaa aaggcgttaa cgcaacgagg ccaggcctac tggtgcaggc aggtgatcag
     2761 agcgagcaaa tttttacccg ctggcgtgct ggggcgtata acgcctggtc gttgcctggg
     2821 cgctgtttta tcgttctcga agagttgcgt tggggggcat ttggcgatgc ctgtcgtctg
     2881 ggaagcccgc aagcggtggc gttgctgctg ggtgatttgc gcgtgaaagc gacacaacat
     2941 ctggcagaga gtatcaatgc ggccccgacc acgcgtcact attaccatca gtggtttgcc
     3001 tcttcgaccg ttctgacggg cggggatcat gctgattttt taagttggct gggaaagtgg
     3061 accacggcag ataaacaacc cgtttgctgg tcagtgaccc aacgttggca aactgtcgcg
     3121 ctggggatgc cacgactctg ttcagcgcag cgtttggtgg gggcaatggt cgaggaaatc
     3181 ttctctgtaa atttggcgta aataatcagt tacatcaatg agtcctaaac gaaatccatg
     3241 tgtgaagttg atcacaaatt taaatactgg tagggtaaaa aggtcattaa ctgcccaatt
     3301 caggcgtcaa ctggtttgat tgtacattcc ttaaccggag ggtgtaagca aacccgctac
     3361 gcttgttaca gagattgcat cctgcaattc ccgctcccct tttgcggccg tcgcgctgat
     3421 ttttctggcg tttgcggaaa tgggccaact ctgcgaggaa agctatgctg aaaaggaaaa
     3481 aagtaaaacc gattaccctt cgtgatgtca ccattattaa tgacagtaaa ctgcgtaaag
     3541 ccattaccgc agcatcactg ggtaatgcaa tggaatggtt cgattttggt gtttatggtt
     3601 ttgttgctta cgcattaggt aaagtttttt tcccgggggc tgaccccagc gtgcagatgg
     3661 ttgctgcact tgccactttc tccgttccct ttctgattcg accgcttggc ggactcttct
     3721 ttggtatgtt gggcgataaa tatggtcgcc agaagatcct cgctatcact attgtgatta
     3781 tgtcgatcag tacgttctgt attggcttaa taccgtccta cgacacgatt ggtatttggg
     3841 caccgattct gctgttgatc tgtaagatgg cacaaggttt ctcggtcggc ggtgaatata
     3901 ccggggcgtc gatatttgtt gcggaatact cccctgaccg taaacgtggc tttatgggca
     3961 gctggctgga cttcggttct attgccgggt ttgtgctggg tgcgggcgtg gtggtgttaa
     4021 tttcgaccat tgtcggcgaa gcgaacttcc tcgactgggg ctggcgtatt ccgttcttta
     4081 ttgctctgcc gttagggatt atcgggcttt acctgcgcca tgcgctggaa gaaactccgg
     4141 cgttccagca gcatgttgat aaactggaac agggcgaccg cgaaggtttg caggatggcc
     4201 cgaaagtctc gtttaaagag attgccacta aatactggcg cagcctgttg acatgtattg
     4261 gtctggtaat tgccaccaac gtgacttact acatgttgct gacctatatg ccgagttatt
     4321 tgtcgcataa cctgcattac tccgaagacc acggggtgct gattattatc gccattatga
     4381 tcggtatgct gtttgtccag ccggtgatgg gcttgctgag tgaccgtttt ggccgtcgtc
     4441 cgtttgtgct acttggtagt gttgccctgt ttgtgttggc gatcccggcg tttattctga
     4501 ttaacagtaa cgtcatcggc ctgatttttg ccgggttact gatgctggcg gtgatcctta
     4561 actgctttac gggcgttatg gcttctacct tgccagcgat gttcccgacg catatccgtt
     4621 acagcgcgct ggcggcggca tttaatattt cggtgctggt tgccggtctg acgccaacac
     4681 tggcggcctg gctggtcgaa agctcgcaga atctgatgat gcctgcctat tacctgatgg
     4741 tagtggcggt gattggttta atcaccggcg taaccatgaa agagacggca aatcgtccgt
     4801 tgaaaggtgc gacaccggcg gcgtcagata tacaggaagc gaaggaaatt ctcgtcgagc
     4861 attacgataa tatcgagcag aaaatcgata atattgacca cgagattgcc gatttgcagg
     4921 cgaaacgtac ccgcctggtg cagcaacatc cgcgaattga tgaataagct gaaacggatg
     4981 gcctgatgtg acgctgtctt atcaggccaa ttgaactctt aaggttcact taatctctga
     5041 cgcgcatact ctcctccagg ttaacggagg agagtgcaat gaaaaaccgt gtttatatga
     5101 aagtttaact accgtgttca gcgtgctggt ggtcagcagc tttctttata tctggtttgc
     5161 cacgttctga tctttcttca gccgtaccca ggcccgcgtg ccggaagtct cttgccggtt
     5221 ttgcaggaaa aactgcccgt gatgcaactg tgtaatgcgg ctgacaatac ttaaccccag
     5281 accaatcccg ccataacggc tgtccatacg tacaaacgct ttactcaact ccccgcattt
     5341 actctcatca atacctggtc cttcatcttc aactgccatg accgctccgc catcttcttg
     5401 cagcttaatc ataatgttgc tgccttgcgg gctgtaacga tgggcgtttt ctaccaggtt
     5461 tcgcaataac atccgcagca gggttgcatc accctgaacg gtgatgtcgg cggcgctctc
     5521 tggcaatagc agggtttgct gtcgctggtc gagcatggta ctgagttcgt catacgaggg
     5581 gagaatgaca tcttccagca gttttacatg ttgataatta ccggaagaaa atgactgtcc
     5641 ggcacgcgcc agttgcagca gctgggagac gctctccatc atctgatcaa gccgtgccac
     5701 taacggtgct acatcaatgt gatgcgtttt cgccagcagt tccagatgca aacgcacccc
     5761 cgccagtggc gttcgcagtt cgtgcgcgac gtcagcggta aacaaccttt cgttatccag
     5821 cgtgctggtc aggcgactga ccagatcgtt caacgccgaa accaccgctt cgatttcgag
     5881 ggtggcgctg tgaatggcaa tgggcgttaa gttgtcggca gtgcgcgctt ccagctcttt
     5941 ttgcagctcc gccagcgggc gggtgatgcg gcgtaccgcc tgatagcaga taaatagcgt
     6001 caggctgacc ataaagacgc cggggacaat caggctggcg accgcctcgc ggatctcacg
     6061 catgatgtgg cgatcgttgt tgcgattgtc gcgtagcgcc tgctcaaaca gctgaatctg
     6121 ctcggtactt tcatgccata gccagaagac gctgatcagc tcaaacacca acaaaatggc
     6181 cccgatagtc aatatcagcc gttggcgcag cgatattggt cggcgcagaa aacgcatcag
     6241 attcaattag ttttcctcat tcgcgaccag catatagcca aagccgcgca cggtgcggat
     6301 acgggatttg cccactttgt cgcgcagatt gtggatatgc acttccaggg tgttggtcga
     6361 gggttcattg tcccagttat agatgtcgtt gtagagaatt tcccgatgta ccggactgcc
     6421 tgctttgagc attaaccgtg acagcagagc atattctttg ggcgtcagaa tcaactcttc
     6481 accgcccatc catacctgac ggcgacccat attcagcgtc agattgccaa caatcagctc
     6541 actttcgccc tgattattat ggcgtcgtag cagggcgcgg atacgggcgt gtaactcttc
     6601 cagcgcaaaa ggcttcacca gatagtcatc ggcaccgaca tccagcccgg cgattttgtc
     6661 ggtcagcgta tcgcgagcgg tgaggatcag taccggcagg gtatattttt tctgccggat
     6721 acgggcgaga aaatgcagtc catcttcgtc gggtaaccct aaatccagta ccaccaggct
     6781 gtaatgaccg gcttcaaggc tttgttccgc catccgcgcg gttgtcacgc catcgcacgc
     6841 gtagccttcg gtttgcgccg ccagaatcag tccctgcaat aacagcgtat cgtcttcaac
     6901 aatcagaatt ttcatttact cactctcctg caagtttgca gaatatcatc cgcagcctgg
     6961 taatacttcg tctcaacgcc agttaatccc aatagcgtgg agaataaatt gtcttgtgaa
     7021 tagtgttgcg tttgcgcctg tttttgcagg cagttctggt caacctgata ccgtttttga
     7081 taatcctccg acagccacag cagcatcggc acctgttttt ggctatccgg ggcgatggca
     7141 taaggcagac cgtgcagata gatgccattt tcacctaacg attcaccgtg gtcagaaaga
     7201 taaaccaggc tggtggtaaa tttatcctga tgttctttca gcagattaat cgctttatca
     7261 acaatatagt cgacgtaaac cagcgtgttg tcgtaagtgt tcaccagttg ctctttggta
     7321 caggtctgga tctcattggt gtcgcaggtt ggggtaaatt tcctgaactg cggcggatag
     7381 cggttgtaat aggtcggacc gtggctgccg atggtgtgta agacaatcac gccatcaccc
     7441 tgcaggttat tgatgtactc ttccagcccg tggaacagca cttcgtcata gcattcgccg
     7501 ttgatgcact gaccaggcag attcagcgcg gtgacgttct ggtgaggcac gcggtcgcag
     7561 gcacctttac agccgccatc gttgtcattc cacagcacgt tgatgcccgc tcgctgaatg
     7621 atatccagca cgccttcctg gtgctgtgcc agctcttctt tgtaatgctc acgcggcata
     7681 tccgagaaca tgcacggtac tgaaactgcc gttgccgtgc cgcaagatac ggtattaggg
     7741 aaatagacca cgttatcttt cgccagccgc gggttagttt cacgcgggta gccgttgagg
     7801 gagaagttct ccgcccgcga ggtttcgccg acaatcagga tggtcaaatt tttacgtttt
     7861 tcgttctgca ttaacgggtt gcggtgggcg tcttcaccaa ttcgcaccag cggtagattt
     7921 gccagtcgct gatgggagta ccatgaccag ctggcaacaa tgctgttaga ggggcttaag
     7981 gatttcacca gctctttgtt attgcggaac aacgaggcgt agtctttata aaacagtgcg
     8041 gcgaccagca aaatcagtag tacagaaacc agaatattgg ctccacggaa aagaacactg
     8101 cgcagacgcg aggtggcagg tttgattttt atccagcagg caatcagcgc agcaagcacg
     8161 ccgctgaatc ccagcgttaa taacatttgc ggtgtcatca gcgcataact ttctgccgga
     8221 gtggtatcaa taatattggc aatcatcgag cggtcgatga cgatgccgta agtcattatg
     8281 aaatattgtg cagccgcgcc aaccagaata aacaggcagg ccagtggtcg attaagccat
     8341 aagaaagagc ttagtgtcag gacaatatta atcacgctga aagcgacgac cggcatcgac
     8401 aagaaaacca gtacgttatg cagcgaatcc agcggcagcg cctgcaacac ctgtttaaaa
     8461 aaggcaatat tcaggcagat agagatataa aaagcggcca acaatagcca ggcgagtaaa
     8521 ttcaaagagg gtctttttag taggcgcttc aacatcacgg tgtttccatc gaacaaagtg
     8581 cgcatatgct cgcaaagcaa aattaagcca accttaagtt cttaaggttg gcttttatgt
     8641 ttgctggatt aaggggaatt aatctttgct tattggtgca tctaagggat acgggttttt
     8701 atgtagccgg ttgtaattca gcgcatacat ggcggtgatg accatcaggg tgacaaatga
     8761 ccacataacc tctttcgctc cggaccccac cacggcccag atgcagtaga ggaaggcaat
     8821 ggtagtaact gccagatatg ccgggcgtgc tttaccaaag tgaccgtgtc cgagcagcag
     8881 taacgccgca caggtgtaaa gatatggcac cagtgtaaag atgaccgaga cggaagaaac
     8941 cagaccgaac tctttggtcg cgtttggtga aatgctgctg agctggaaga tggtcatcaa
     9001 aataccgacg ataatcagcc ccgccactgg cgtacccgct ttatttacac gggcaaaaat
     9061 cggtgggaac agtccgtcat cggcagcggc tttcgccgtt tgacccgcca gcaacgtcca
     9121 gccgcccagt gaacctaagc aacccgcagc tgcgcagaag gaaacaatgg ccccgggggt
     9181 gtcacccaac gccatccgtg cggcatcacc gaatggcgaa gcagaaacgc gcagtgcggc
     9241 attagggatc atccccataa tcgcggtggt agaaagtaca tagcaaacgg cggcaatcaa
     9301 taccccacca atggtggcga tagggacatt gcgtttcggg tttttcacca cacctgcggc
     9361 aacggaggca ctttccacac cgatgaacga ccacagcgta acgttaaggg tactttgaat
     9421 tgcaccgaag gtacccaggc cgctgacgtt ccatgccgcc atataggttt caccacggaa
     9481 ccagaaccag ccaaatacgg caatcccgac gatggggatc agcgccagca cggtggcaac
     9541 tgcctgcaca cgggtgatca ttttcggacc aacaatgttc agcaggacga agatccacag
     9601 caccacgacg caggtgatgg ttaataccaa tggatctttc agaatcggga agaagtaact
     9661 taaatatcct acgccaatga ccaccatggc gatattgccg atccagcagg ccagccagta
     9721 gaggacgttg gtttgataac cgagaaacgg gccaaagcag cggcgggcgt aagcgtaaga
     9781 accaccagga cttgggtcga ggaacgacat tttggcgtat accatcgaga gccccagtgc
     9841 accgataatc gtcaccaacc atccataaat ggcaatcccg ccagtggagg ccaggtttgc
     9901 aggtaacaga aaaacacctg accccataat attccccgac accatcaggg tgacggggat
     9961 taagcccact ttgtgagcat cagcatccga agacataatt aaactcctgc gaaggcgagc
    10021 ttcgtgacaa taaattacgt catatcatac gcctgcattc gatagttgaa ttattatcga
    10081 ccgggttatc gctgatgccg ttattaatat agctcttcgt taaggttaaa aatcaggcga
    10141 cagtgcgttc tctgtgctga ctaacataat gcagcggcgt cataccgtag aagtctttaa
    10201 agacagaaat aaagtacgac gtactgttgt agccgcagga ctgtgatacc tgagagatat
    10261 ttttaccgtc catcattaat tcatttacgg cataacgcat gcggcaggtg gtgattattt
    10321 ggctataact ggtgttttcg cttttcagct ttttctttaa cagacttggg ctaagacata
    10381 aacaactggc taccatacta agattccagt ctttgtgaat atcgctttca ataatttgat
    10441 aaacgctgtc acttacacaa ttacgtaaca tatacatcat taatgaaagg aattttttac
    10501 tgtcgagaaa acgggatagc accgtgaata ataatgcgcg tgttcgttcc ttttctgact
    10561 cagtttctgc gggcatcatg ctgtgttgcg cggcaacgcg aaagacgtct ggcgtcaggc
    10621 atggcagggt gagtatgggc gtagcgctac gttgccatac tggtatttgc gagagatctt
    10681 tattcagaaa ccggaggtaa tctttgatga tgtcgtgact aatatgagca accaaagtgt
    10741 tgttaagaga ggagacgtcg ataatattat tttcacaatt taataacgcc atatggttag
    10801 ctttaaggct aataggttct ttcccattca cccttatcca gacatctttt tcagtcaata
    10861 aaacaataca aggttggtcg ctgcaaatcc tcatacatgt actcctgagt gcgaataaaa
    10921 gaccagttct cgctaaagca aagcgatacc gagaaatggt tgtataattg tatgacgaat
    10981 ataaacatgc ataaaaataa cgagttttac taatattaca aatttttata gtaatttctc
    11041 atgtacatat ttttttacag ggtaaataac aggcaaaaaa aagcgcgggg tggggccgcg
    11101 caaaaagtat gacatttttg tttgcagcaa tttttatgaa aacgttgcca tactcaaaag
    11161 gcctaaggcc aggcggcgta atgttattta aacaattacg ccttcagcgg aatagtggtt
    11221 acgctttcac acacatagcg tggtaaatac cgtcaataat ttcagtccct tcagtttcgt
    11281 gttcaaatcc agggaaatgg tggtcccagg attgcagcga gcgtaaataa cttacttgcg
    11341 gactgttttt atcgccgaag ttttcaccag acagcagcat cgggattcct ggcggatatg
    11401 ggataactga gtttgccgcg atgcgtcctg gcagattttc aatggatacc agttcgacat
    11461 tgttgtcgac aatcgcgttg tacgcttcac gcggggtgat ttccgccacc ggcaggccgg
    11521 aataggcttc gttcaaccgt gcgccagggt tgttttcttt cagccaggca aacatggtgt
    11581 cacccagatc gtgaatcccc atgttcgcgt aagtgtcagg atattgttca acaagttccg
    11641 gcatcacctg cgccagcggt gtgttggcgt catagtggcg tttgaaggag caaagggtgt
    11701 taaccagagt cccccatttc ccacgggtta cgcccataga gaacaggaac ataatttgga
    11761 agtcagtggt gcgggtaggc acaatgccgt ggcgaccaag ccaggcagtg accagcgccg
    11821 ccggaacacc ggtttcttcc agttcaccat cttcacccat tcccggagca aggatgctga
    11881 ctttaatcgg gtcgagcata ctccagttat ccggaatatc tttgaagccg tgccagcttt
    11941 cgcccggatg cattacccag tagtcctgaa cggtggtcag cagtttggtt ggtgcgtcag
    12001 caaagtcata ggttttgccg gtttgtgggt cggtgacgac ttctttgttc cacggtttga
    12061 agaaccagct accgtcagcg gtgaactctt tatacagccg cgccatcgcc tgacggaaat
    12121 caaccgcttc gtcgatcact tcctgcgtca gtgacaggcc gctgttgccg tccatcatcg
    12181 acaccgccac gtcgttggat gcgcagatgg catacagcgg ggaggtggtg gcatgcatca
    12241 tgtaggcctg gttgaagcgg gagaagttaa tcgccccacg accttcacgt acatgaatat
    12301 aagaagcctg tgaaagcgca ttcagcagtt tgtgggtgga gtgggtggcg aaaacggtag
    12361 gaccgttgtg atcgccaggt tcgccgcgca tggcatagtg atcggcatag atcgggttga
    12421 aacgtgcata gccgtaccag gcttcgtcaa agtgcagacg atcggaggtt ttttccagca
    12481 gatcctgcgc ttctttagcg ttataacaca cgccgtcata ggtgcagttg gtcaccacgc
    12541 agtaagacgg tttttgcccg gctttgtctt tagtcagcgg gctttcactg attttcttct
    12601 gcaaggtttc aggttgcatt tcctgcggat agattggccc gataatgccg tagcggttgc
    12661 ggcttggcac catgtagacc ggtttcgcgc ctgtcagcat caagccttgt tcgatggatt
    12721 tatggcagtt acggtcaacg accacgacat cgttatcggt catgcaagcc tgcatgatgg
    12781 tgcggttaga gccggaagta ccgacgacta ccgaccagga gcgatcggca ccaaatacgc
    12841 gtgcggcata tttttcgctt tcgccaaatg cgccagtatg gtcaagcaaa gaaccgaggg
    12901 aagttcgttc gatgcccata tcggtgcgga acagattttc tccatagtag tcatggtaga
    12961 aacgtccggc gggtgttttg gtaaaaccaa cgccgccctg gtggcctggc gctgcccagg
    13021 aatattcatg gatgtcgcta tacttcatca gcgcgctgaa cagtggcggc aacagctgct
    13081 ggcggtagcg ggtcatcgcg gcaacggcgc gtccggcgat aaagtcggtg gtatcttcca
    13141 gaatccaggc gaattcatcg acaagctcca gcaggtcgcg atccattgcg gcgagggctt
    13201 tttcccgatc gcccaacagg aagaccggca cgttttgttg gcgctcatga agcttaccga
    13261 tcaattgtct gacgttttga tgttcgtccg gatgttccat ttgatagctg aacatcaggc
    13321 agtcaatggc ttcgtttgaa gagagaatgg caaaaccatc atcaaaggag gtggatttaa
    13381 tcacggtaac attttgctgg cttaaagcat ctgccagacg ctcaacggcg ttaccgaccc
    13441 aggtgtcttg atggagaaac tcgctttcaa caattaatac tttcatcatt gcttacccgg
    13501 ttatgaagga atcttcgttg cgggcaagta tcttccggcc ttttcgggct gtaaagcgcg
    13561 tgaaaaacaa acatttttga aatatttatt tttataaaca atgcgttatg tgattatttt
    13621 aaattatttt cactgcttgt tatcgacggc taaactattt ttttggctga tactgatatc
    13681 gtctttagcc gggaaacgtc tggcggcgct gttggctaag tttgcggtat tgttgcggcg
    13741 acatgccgac atatttgccg aacgtgctgt aaaaacgact acttgaacga aagcctgccg
    13801 tcagggcaat atcgagaata cttttatcgg tatcgctcag taacgcgcga acgtggttga
    13861 tgcgcatcgc ggtaatgtac tgtttcatcg tcaattgcat gacccgctgg aatatcccca
    13921 ttgcatagtt ggcgttaagt ttgacgtgct cagccacatc gttgatggtc agcgcctgat
    13981 catagttttc ggcaataaag cccagcatct ggctaacata aaattgcgca tggcgcgaga
    14041 cgctgttttt gtgtgtgcgc gaggttttat tgaccagaat cggttcccag ccagagaggc
    14101 taaatcgctt gagcatcagg ccaatttcat caatggcgag ctggcgaatt tgctcgttcg
    14161 gactgtttaa ttcctgctgc cagcggcgca cttcaaacgg gctaagttgc tgtgtggcca
    14221 gtgatttgat catcatgccg tgagtgacgt ggttaatcag gtctttatcc agcggccagg
    14281 agagaaacag atgcatcggc agattaaaaa tcgccatgct ctgacaggtt ccggtatctg
    14341 ttagttggtg cggtgtacag gcccagaaca gcgtgatatg accctgattg atattcactt
    14401 tttcattgtt aatcaggtat tccacatcgc catcgaaagg cacattcact tcgacctgac
    14461 catgccagtg gctggtgggc atgatatgcg gtgcgcgaaa ctcaatctcc atccgctggt
    14521 attccgaata cagcgacagc gggctgcggg tctgtttttc gtcgctgctg cacataaacg
    14581 tatctgtatt catggatggc tctctttcct ggaatatcag aattatggca ggagtgaggg
    14641 aggatgactg cgagtgggag cacggttttc accctcttcc cagagaggcg aggggagcgt
    14701 ccgagcatca tgaggccgaa aactctgctt ttcaggtaac ttattaccat aaactcagat
    14761 ttactgctgc ttcacgcagg atctgagttt atgggaatgc tcaacctgga agccggaggt
    14821 tttctgcaga ttcgcctgcc atgatgaagt tattcaagca agccaggaga tctgcatgat
    14881 gactgcaccc aaaattacat ttatcggcgc tggttcgacg attttcgtta aaaatattct
    14941 tggtgatgtg ttccatcgag aggcgctgaa aacggcgcat attgccctga tggacattga
    15001 tcccacccgc ctggaagagt cgcatattgt ggtgcgtaag ctgatggatt cagcaggggc
    15061 cagcggcaaa atcacctgcc acacccaaca gaaagaagcc ttacaggatg ccgattttgt
    15121 cgtggtggca tttcagattg gcggttatga accttgcacg gtgactgatt tcgaggtctg
    15181 taagcggcat ggtctggaac aaaccattgc cgatacgttg gggccgggcg gtattatgcg
    15241 cgcgctacgt accattccgc atctgtggca aatttgcgag gacatgacgg aagtctgccc
    15301 cgatgccaca ttgctcaact acgttaaccc aatggcgatg aatacctggg cgatgtatgc
    15361 ccgctatccg catatcaaac aggtagggct gtgccattcg gtgcagggaa cggcggaaga
    15421 gctggcgcgt gacctcaata tcgatccgtc cacgctgcgt taccgttgcg ctggtatcaa
    15481 ccatatggcg ttttacctgg aactggagcg taaaaccgcc gacggcagtt acgtgaatct
    15541 ctacccggaa ctgctggcgg cttatgaagc agggcaggca ccgaagccac atattcatgg
    15601 taatactcgc tgccagaata ttgtgcgcta cgaaatgttc aaaaagctgg gctacttcgt
    15661 cacggaatcg tcagaacatt ttgccgaata tacgccgtgg tttattaagc caggtcgtga
    15721 ggatttgatt gagcgttata aagtaccgct ggatgagtac ccgaaacgct gcgtcgagca
    15781 gctggcgaac tggcataaag agctggagga gtataaaaac gcctcccgga ttgatattaa
    15841 accgtcacgg gaatatgcca gcacaatcat gaacgctatc tggactggcg agtcgagtgt
    15901 gatttacggc aacgtccgta acgatggttt gattgataac ctgccacaag gatgttgcgt
    15961 ggaagtagcc tgtctggttg atgctaatgg cattcagccg accaaagtcg gtacgctacc
    16021 ttcgcatctg gccgccctga tgcaaaccaa catcaacgta cagacgctgc tgaccgaagc
    16081 tattcttacg gaaaatcgcg accgtgttta ccacgccgcg atgatggacc cgcatactgc
    16141 cgccgtgctg ggcattgacg aaatatatgc tcttgttgac gacctgattg ccgcccacgg
    16201 cgactggctg ccaggctggt tgcaccgtta aaacgcgact aaacgctact gcgccggggg
    16261 atttattccg gcgcacacct ctgacgatac caataacaga aggcgggcgt tggtaacagc
    16321 gacccgatac cctatgagca tttcaatgac tacaaaactc agttatggat ttggagcgtt
    16381 cgggaaggat tttgcgatcg gcattgtgta tatgtacctc atgtattact acaccgatgt
    16441 cgttgggctg tctgtgggtt tggtcggtac tttgtttctg gtggcgagga tctgggatgc
    16501 tattaacgat ccgattatg
//
LOCUS       DAJTMC010000089        15966 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.089, whole genome shotgun sequence.
ACCESSION   DAJTMC010000089 DAJTMC010000000
VERSION     DAJTMC010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15966
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.089"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(<1..243)
                     /locus_tag="OO130_002532"
     CDS             complement(<1..243)
                     /locus_tag="OO130_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCR5365894.1"
                     /translation="MSSWLAMLQDAAEMFVFLAVELSLLFIVISAGVSLIRQKVPDHK
                     IQQMMGARKGRGYLLAALLGAVTPFCSCSTIPMLRGL"
     gene            350..646
                     /locus_tag="OO130_002533"
     CDS             350..646
                     /locus_tag="OO130_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5365895.1"
                     /translation="MQLEEVAKALKELGHPTRLFIFKHLVKAGEQGLPVGELQKQLGI
                     PGSTLSHHISALVSVGLVKQNRESRTLMCVSQYAMLEAIIEFLREECCVNSKIA"
     gene            complement(780..1205)
                     /gene="arsC"
                     /locus_tag="OO130_002534"
     CDS             complement(780..1205)
                     /gene="arsC"
                     /locus_tag="OO130_002534"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HCR5365896.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(1218..2507)
                     /gene="arsB"
                     /locus_tag="OO130_002535"
     CDS             complement(1218..2507)
                     /gene="arsB"
                     /locus_tag="OO130_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HCR5365897.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(2562..2915)
                     /gene="arsR"
                     /locus_tag="OO130_002536"
     CDS             complement(2562..2915)
                     /gene="arsR"
                     /locus_tag="OO130_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HCR5365898.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKIQAIVRN
                     LARQNCSADSKNICS"
     gene            3654..3737
                     /locus_tag="OO130_002537"
     CDS             3654..3737
                     /locus_tag="OO130_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HCR5365899.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(3791..5143)
                     /gene="gorA"
                     /locus_tag="OO130_002538"
     CDS             complement(3791..5143)
                     /gene="gorA"
                     /locus_tag="OO130_002538"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HCR5365900.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKMLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSEHLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGSEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(5215..6057)
                     /gene="rlmJ"
                     /locus_tag="OO130_002539"
     CDS             complement(5215..6057)
                     /gene="rlmJ"
                     /locus_tag="OO130_002539"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HCR5365901.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATASW
                     IVPE"
     gene            6260..8302
                     /gene="prlC"
                     /locus_tag="OO130_002540"
     CDS             6260..8302
                     /gene="prlC"
                     /locus_tag="OO130_002540"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HCR5365902.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTQALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAELAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELAQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            8310..9062
                     /gene="rsmJ"
                     /locus_tag="OO130_002541"
     CDS             8310..9062
                     /gene="rsmJ"
                     /locus_tag="OO130_002541"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HCR5365903.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVTTPNAVVTKGHRFDIYAGTPV"
     gene            complement(9111..10580)
                     /gene="dtpB"
                     /locus_tag="OO130_002542"
     CDS             complement(9111..10580)
                     /gene="dtpB"
                     /locus_tag="OO130_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HCR5365904.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADRFGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFKPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINSVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            complement(10898..11332)
                     /gene="uspA"
                     /locus_tag="OO130_002543"
     CDS             complement(10898..11332)
                     /gene="uspA"
                     /locus_tag="OO130_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HCR5365905.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            11723..12058
                     /gene="uspB"
                     /locus_tag="OO130_002544"
     CDS             11723..12058
                     /gene="uspB"
                     /locus_tag="OO130_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HCR5365906.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(12302..13801)
                     /gene="pitA"
                     /locus_tag="OO130_002545"
     CDS             complement(12302..13801)
                     /gene="pitA"
                     /locus_tag="OO130_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HCR5365907.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKIPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            14033..15235
                     /locus_tag="OO130_002546"
     CDS             14033..15235
                     /locus_tag="OO130_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCR5365908.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLELNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLIAAKSS"
     gene            complement(15550..>15966)
                     /locus_tag="OO130_002547"
     CDS             complement(15550..>15966)
                     /locus_tag="OO130_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 family protein"
                     /protein_id="HCR5365909.1"
                     /translation="QTRNTFSTKEHWLWCYWVIFLGSITVLQGIYVLVSSDASARLAP
                     GIILIYLGMICYSIFSKVWLLALVWRRTCSLANRIPMIPVFTCLFCLFLASFLAEMAQ
                     TDMGYFIPSRVLVGLGAVCFTLFSIVSILEAGSAKK"
BASE COUNT         4057 a         4073 c         4170 g         3666 t
ORIGIN      
        1 caatccacgt aacatgggta ttgtcgagca actacagaac ggggtaacgg ctcccaacag
       61 agcagccagg agatagcctc tcccttttct ggcccccatc atctgctgga ttttatggtc
      121 tggcaccttt tgtcttatca ggctgacacc ggcactaatc actataaaca gcaacgaaag
      181 ctcgacggcg agaaacacaa acatctctgc ggcatcttgc agcatcgcaa gccaactact
      241 catacgtaac ctcattggat aaatctatat ttccagaata atcgaaatga aattcagatc
      301 aagatatttc tggtattatc gaaatatcaa ccagacagag gggtgtcata tgcagttgga
      361 agaagtcgct aaagcgctga aagagctagg tcacccgacc cgtttgttca tatttaaaca
      421 tcttgtcaaa gccggggaac aagggttgcc ggtcggtgag ttgcaaaagc agcttgggat
      481 acctggctcc acactcagcc accatatatc agcactggta tcggtaggac tggtcaaaca
      541 aaaccgtgag agtcggacgc tgatgtgtgt ttcgcaatac gcaatgctgg aagcgataat
      601 tgaattctta cgcgaggagt gctgcgtaaa tagcaaaatt gcctgatgcg ctacgctcta
      661 catatttcat gccatatatt gaatttgcac ggttttgtag gtcggataag acgcgttagc
      721 gtcgcatccg acataaacaa agcgcacttt accaactatc ggcagtggat atcgccgctt
      781 tatttcaggc gcttacccgc ttcatcaacc actttctcgc catcttcctt ggagaatgcg
      841 cctttttgcg catctggcag aatttccagc accacttctg aagggcggca caggcgagtt
      901 cccagcggcg tcaccacaat cgggcgatta atcagaatcg ggtgctgaag cataaagtcg
      961 attaaccgat cgtcagtaaa tttatcttcc gcaaggccca gctcctcata cggttcgacg
     1021 tttttacgca gcagcgcgcg tacggaaatc cccatatcgg caatgagttt gaccagttca
     1081 tcgcgcgttg gcggagtttc cagataatgg ataatggtcg gttctgtgcc gctgttgcgg
     1141 atcatctcca gcgtattacg cgacgtgccg caggccgggt tgtgataaat ggtaatgttg
     1201 ctcatatcag tatctcatta caaagtgaaa gagagacgta gcgccagcgc agccagcgtc
     1261 acaaacagca caggcagggt catgataatc cctgtacgga agtaatatcc ccagctgata
     1321 gtcatattct tctgcgaaag tacgtgcagc cagagtagtg tagccaggct accaattggg
     1381 gtaattttcg gtcccaaatc gcagccaatc acattggcat aaaccatcgc ttctttgata
     1441 acgccagatg ccgtgctgcc atcaatggac aacgcgccaa ccagtaccgt cggcatattg
     1501 ttcataatag aagagaggaa ggcggtgagg aatccggtgc cgagcgtcgc ggcccacagg
     1561 ccgttatccg ccagcacgtt gagtacgcca gaaagatatt ccgttaatcc ggcattgcgc
     1621 aggccataaa ccaccagata catgccgagc gagaagatga caatttgcca gggggcaccg
     1681 cgcaggactt tacccgtatt aatcgcatga ccgcgtttag cgacgacaaa taatatcagc
     1741 gcgcccacag ctgcaatggc gctcaccgga atgccgagcg gttccaggac gaaaaatccc
     1801 accagcagaa gcagtaaaac aacccagcca gttttgaacg tagcaggatc tttgatcgct
     1861 tctgcgggag atttcagcag cgccatatcg tagttctgcg gaatatcttt gcgaaaatag
     1921 agatgtagca tcaccagcgt ggcaacaatc gcggcgatat ccaccggcac catcaccgag
     1981 gcgtattcgc gaaagccgag gccaaagaaa tcagcggaaa cgatattcac caggttggag
     2041 acaataagcg gcaggctggc ggtatcggca atgaatccgg ccgccatcac gaacgccagc
     2101 gtcgtgcctt tactgaatcc taaagccagc agcatggcga tgacaatcgg cgtcaaaata
     2161 agcgctgcgc catcattggc aaacagggcg gcaacggcag caccgagcag gacaatccag
     2221 gtaaacagca agcgaccacg accattaccc cagcgtgaga cgtgcagcgc cgcccattca
     2281 aaaaagccgg actcatccag cagcaggctg atgataatga cggcgataaa cgcagccgtc
     2341 gcgttccaga cgatattcca caccaccgga atatcacccg gatggaccac gcccgtaact
     2401 aacgctagta ctgcgccgag cgttgcactc cagccgatgc ctaaaccttt cggctgccag
     2461 ataaccaata caatggtcag gacaaagata gcgcctgcca gtaacataat gcctcctgga
     2521 taaaacacat gcgaaaatac atatgtgttt agctaaattt tttaactgca aatgttctta
     2581 ctgtctgcgg aacagttttg tcgagccagg ttgcggacaa ttgcctgaat cttttcctgt
     2641 tcacatcgcc aggcttcatc aattattttc gccgcccatg ctggaatatg cggcgataag
     2701 cggtaatgaa cccatttacc ttgcttgcgg tccagcaata gcccgctttc acgcagcaat
     2761 gccaggtggc gggagatctt gggctgcgac tggtcgagag cagtgcagag atcgcagacg
     2821 cataactctc ccagttcgct gagcagtaaa acgatgccca gacgggtttc atcagcaaga
     2881 attttgaaca attggatggg taacagaaat gacatattgc gctcctgatt gttgcaggta
     2941 gtgtctctct tcgaagcgga taagtcaaaa acatatatga tttaacgaat gtgtaagtgc
     3001 agaggaaggt aataggtgtg aattttgagt tggctattca tttgaaagga ggtctgaatc
     3061 aggacgtaaa aaaaccgact ttgcgtcggt tttttacttt ccagccctga gttggtggct
     3121 ctggctggag tgagagagtt cttatctaac agctcaatac tattagccag tggctaacat
     3181 tgcaagaatt agcgatttct tcagctggcg gagggagcac ttcggtatat atcgcagtga
     3241 cgatttatat actttcactg ggtcatcgtc atattaagcc ttgtccgaat ccggatgtaa
     3301 aaaaccgact ttgcgtcggt tttttacttt ccagccctga gttggtggct ctgactggag
     3361 tgagatagcc atcatctaac gcatcaatgc taataccata ctgaaactat tgcaaggacg
     3421 tgctggtttt ataacctgca tgtactgtat gattatccag ttagctctga ggcattttca
     3481 ctctggcaat gcgcataaac gctttcaaag tcctggtcag aagtacgggt ggtgccgtta
     3541 actgatgctc tggccggagt gagagagttc ttatctaaca atgagacatg cgccgtgaca
     3601 ggcagtggat gagtaagcgg atgcattctc actccatcgc atggagaaaa cgggtgattg
     3661 ataaagcaat catcgttcta ggggcgttaa ttgcgctgct ggaactgatc cgctttctgc
     3721 ttcagcttct gaactgatag cggaaacgta attaagggct aagagcacac tactcttagc
     3781 cctttaacat ttaacgcatt gtcacgaact cttctgccgc cgttgggtga atggcgacgg
     3841 tattgtcgaa gtcttttttg gttgccccca tcttcagcgc caccgcgaag ccctgcaaca
     3901 tttcgtccat accaaagcca atgccgtgaa taccgacaat cttctcttcc gatccaacgc
     3961 acaccagctt catgcggcac ggctggcggt gagtggtgac ggcggtatac atcgcggtga
     4021 aagaggattt atacactttc acctgatcgt cgccatactg ctcgcgcgcc tgcggttccg
     4081 ttaaaccaac agtaccaatc ggcggatggc tgaagaccac ggtcggaatg ttgctgtaat
     4141 ccagatgctc atccggcttg ttattaaaca ggtgttcaga gagacggcga cccgctgcaa
     4201 ctgccaccgg tgtcagctcc actgcacccg tgttatcgcc caccgcgtaa ataccttcaa
     4261 tgttggtgtt ttgatattta tcgacgacga tatagccttt ttcgttggtt ttaacgccag
     4321 cggcttcgag gttgatgttg tcattggcag gctcgcgacc aatcgcccaa atcaggcaat
     4381 ccaccgtttc actgcgacca tcttccagct ccagcgtcag gctaccatcg gcatttttca
     4441 ctaccgcttt cgggatggcg ttggtgtgca gctgcgggcc ttcggcgttc atcacttcga
     4501 ccagcgtttc ggaaatcatc gggtcgaagc tgcgcagcgg cgcatgttta cgcacaaaca
     4561 gatgcgtttt cgcgccgagg ccgttaatca cgcccgccag ctcaacggcg atgtaacccg
     4621 cgccaacaac cgccacgcgc tctggcaaag caggaagggc gaagaagcca tcagaatcaa
     4681 taccgtattc cacgcccgga atatccgggt ggctcggacg accgcctgtg gcgatcagaa
     4741 tatgatcggc cgtgatggtt tcgccgttta cctccagcat tttggcatca acgaagcggg
     4801 caaagccttt gattacatca acgttatttt taccgagcac gttttcatag gaagtatgaa
     4861 tacggtcgat ataggcggta cggctggcga tcaacgtttc ccagttgaac ttgttgatgg
     4921 tggtatcgaa accataatca gggccgtaca tatggatcgc ttcacggatt tgcgccgcgt
     4981 gccacatcac ttttttcggc acgcagccga cgttcacgca ggtgccgccc agctctttgg
     5041 cttcaatcag tgcgcatttc tggccgtaca tagccgcgcg gttgatggag gctataccgc
     5101 cgctgccgcc gccgatggcg atgtaatcat agtgtttagt catgacagag tgtccttatc
     5161 gttgattacc gcgattgtag cgcgagacgt aataggtgcc agcaatggct gcaattactc
     5221 cggcacgatc cagcttgcgg tggcgtgccc ggtgcctgcc ggaaccagtt tgctgtgcag
     5281 ccacggcagc acgttattca tctgctgttc cagtttccac ggcgggttaa tcacaatcat
     5341 gccggaagcg gtcatgccac ggcgatcgct gtctggcagt accgccagtt caatttgcag
     5401 aattttgcga ataccggtcg cttccagatc gtggatcatg cgcttaattt gctgacgcag
     5461 caccaccgga taccacagtg cgtaagtacc agtggcgaaa cgtttgtaac cttctgctat
     5521 cccgctgacc accgcctgat agtcggtttt catttcatac ggcgggtcga taaggattaa
     5581 accacggcgg gaaaccggcg gcagtttggc cttaagctgc tggaaaccat cggctttttc
     5641 gacgcgcgca cggctgtctt tctgaaattc agaacgcagc aacgggtaat cgctcgggtg
     5701 cagttcggtc agttgcaggc tgtcttgttc gcgcagtagc tggcgagcaa tcaatggcga
     5761 accggggtag taacgcaact gaccgctacg gttgaagtgt tttaccacat tgatgtacgc
     5821 ctccagttct gcgggcaaat cgtcctgctg ccagatacgg gcgatgcctt cgagatattc
     5881 gccggtacgc tcggcatgtt cgctgcctaa ctgataacgc ccggcccctg cgtgggtgtc
     5941 gagatagaga aacggtttat ctttctcttt cagcgactcg atgatcaggc tctgaacggt
     6001 atgtttaagg acgtcggcgt ggttgccagc gtgaaagctg tggcgataac tgagcatggg
     6061 taaaggtgtt ccggtaagta aaaatcggcc cgtattcggg gcgcacaaaa gcgtatcagg
     6121 acagtatacc gaaaagaggc cgccgccgcg aaagcgtaac gtttctcatt gaaattcact
     6181 acacttaacc ccatgctaca cacattatgt aaagcgcctg ttgagcgctt ccttaacctc
     6241 tttaaccagg actgcgcgaa tgacgaatcc gttactgact ccctttgaat tgcctccgtt
     6301 ttctaaaatt ctcccggaac atgtcgttcc agccgtgact caggcgctga acgattgccg
     6361 cgaaaatgtg gagcgcgtag tagcgcaagg ggcaccgtac acctgggaaa atctctgcca
     6421 gccgttggcg gaagtggacg atgtgttggg gcgtatcttc tccccggtca gccacctgaa
     6481 ctcggtgaaa aatagcccgg aactgcgtga agcctacgag caaaccctgc cgctgctgtc
     6541 ggaatacagc acctgggtag ggcaacatga agggctgtat aaagcgtacc gcgacctgcg
     6601 cgatggcgat cattacgcca cgctgaacac ggcgcagaag aaagcggttg ataacgccct
     6661 gcgtgatttt gaactctctg gcataggtct gccgaaagag aaacagcagc gttacggcga
     6721 aattgcgacc cgtctttctg aactgggcaa ccagtacagc aacaacgtcc tcgatgcgac
     6781 aatgggctgg accaaactcg ttaccgacga agcggagctg gcggggatgc ctgaaagcgc
     6841 gctggctgcg gcaaaagccc aggccgaagc gaaagagctg gaaggctacc tgctgacgct
     6901 ggatatccca agctacctgc cggtaatgac ctactgcgac aaccaggcct tgcgtgaaga
     6961 gatgtatcgc gcttacagca cccgcgcctc cgatcaaggc ccgaacgccg gtaaatggga
     7021 taacagcaag gtgatggaag agatcctcgc tctgcgtcac gaactggcgc aactgctggg
     7081 ctttgaaaac tacgccttta aatcccttgc cactaaaatg gcagaaaacc cgcagcaggt
     7141 gctggatttc ttaaccgatc tggcaaaacg cgcgcgtccg caaggcgaaa aagagctggc
     7201 acaactgcgc gccttcgcca aagcggaatt tggcgtcgat gagttgcagc cgtgggatat
     7261 cgcgtactac agcgaaaaac aaaaacagca cctctacagc atcagtgacg aacagctgcg
     7321 tccgtacttc ccggaaaaca aagcggttaa cggcctgttt gaagtggtga aacgtattta
     7381 cggcatcacc gctaaagagc gtaaagatgt tgatgtctgg catccggatg tacgtttctt
     7441 cgaactgtat gacgagaaca acgaactgcg cggcagcttc tacctcgatc tgtatgcccg
     7501 tgaaaacaag cgcggcgggg cgtggatgga tgactgcgta ggccagatgc gtaaagctga
     7561 cggttctctg caaaaaccgg tcgcgtattt gacttgtaac ttcaaccgcc cggtaaatgg
     7621 taaaccggcg ctgttcactc acgacgaagt gatcaccctg ttccacgagt tcggtcacgg
     7681 cctgcaccat atgctgaccc gcatcgaaac cgctggtgtt tccggtatca gcggtgtgcc
     7741 gtgggatgcg gtcgaactgc cgagtcagtt tatggaaaac tggtgctggg agccggaggc
     7801 gctggcgttt atctctggtc actatgaaac cggcgaaccg ctgccgaaag agttgctgga
     7861 taaaatgctg gcggcgaaga actaccaggc ggcgctgttt attctgcgcc agctggagtt
     7921 cggcctgttc gatttccgcc tccatgccga gttccgcccg gatcagggtg cgaaaatcct
     7981 cgaaactctg gcagaaatca agaaactggt tgccgtagta ccgtctccat cctggggccg
     8041 tttcccgcac gctttcagcc atattttcgc cggtggttat gccgcaggtt actacagcta
     8101 cctgtgggct gacgtactgg cggcagatgc cttctcgcgc tttgaggaag agggcatttt
     8161 caaccgtgaa accgggcagt cgttcctcga caacattctg agccgtggcg gttcagaaga
     8221 gccgatggat ctgttcaaac gcttccgtgg tcgtgaaccg cagctggatg cgatgctgga
     8281 gcattacggc attaagggct gatcattcag tgaaaatctg cttaattgat gaaacaggcg
     8341 ccggagacgg cgccttatct gttctggcgg cccgctgggg gctggagcac gatgaagaca
     8401 acctgatggc gctggtgtta acgccggaac atctggaatt gcgcaagcgt gatgagccaa
     8461 aacttggcgg catctttgtt gattttgttg gcggagcgat ggcgcaccga cgcaaattcg
     8521 gcggtggtcg cggtgaggcg gtggcgaaag cggtgggcat taaaggcgat tatttgccgg
     8581 atgtggtgga cgccaccgca gggctggggc gcgatgcctt tgtactggct tcagtcggct
     8641 gccgcgtgcg gatgctggag cgtaatccag tggttgccgc gctactcgac gacggcctgg
     8701 cgcgtggtta tgcggatgcg gaaatcggcg gctggttgca ggagcggttg cagttaattc
     8761 acgcctccag cctgacggcg ctaaccgata ttaccccacg cccgcaggtg gtctatctcg
     8821 atccgatgtt cccgcataag cagaaaagcg cgctagtgaa aaaagagatg cgtgtctttc
     8881 agtcgctggt gggaccggat cttgatgccg atggattact ggagcctgca cgcttgctgg
     8941 cgaccaaacg cgtggtggta aagcgcccgg actacgcgcc accgctagcg aatgtcacca
     9001 cgccaaacgc ggttgtgact aaagggcatc gctttgatat ctatgcaggt acgcccgtgt
     9061 aaaagtaaag cagggtagcg ataacgctac cctgctttct gcaagaataa ttaatggctt
     9121 tccggcgtcg caatcatgcg tttcagccac ggcaccatca acagcatcac tactgcaacg
     9181 cccagcgtga ccagaccaat cttaccaaac acgttggtat agacgggcaa cgtctcaagc
     9241 ggatcggtaa tgttgtccgg caccgcggta aatgttgcca catagccgcc cagcaagaac
     9301 gcggcagcct gcgtcaggaa ccacatcccg agaataaagc ccatcaaatg ctgcggcacc
     9361 agggcagcaa tcatcgccag gccaagggcg ctaataaaca gttcacctaa gctctggaat
     9421 aagtacacca gcacgataaa ccatggcgat gtcagccctt gtgcatccgc aaaccacatt
     9481 cccgcagctg ccgccgtcaa aaagcccagt gagcacataa acatgccgag agtaaatttc
     9541 atcggcatcg agaggtcttt gcctttgtta cccagatgcg tgtaaatgcc tgccagtatt
     9601 gggctggcga gtaccaccca gaacgggtta agcgcctgga agctgaccga gttgatggaa
     9661 aaaccgagaa tttcatgatg cacgttgttg atggcaaaga agttcagcga tgttggcatc
     9721 tgggcgtaga gaatgtaaaa caccaccgct tcgagcatca ggacaaaggc gacaaacatt
     9781 ttattgcgcc cggttttatc cagcttgaat gcctgacgaa agaagatgat ggtgacgacg
     9841 atggagagaa caatcagcac cagattggcg acttctacgt tgtgcatcag ccatgcacat
     9901 acgaagatca tcaccacgct gccaagtaat acgtacaaca gtttgctgaa gctcatcggc
     9961 ttgaagtcgg gttcagaacc aatgtctttc accattccac gacaggcgat gtaaaccagt
    10021 aatgcgataa ttaaccccgc cccgcacagg ttgtaggtga ctgaataacc gaatctatca
    10081 gcgatcacag gggccagcga taacgctatc aacgagccga tattgatcga catatagaac
    10141 agggtgaatg cgccatcaag ccgcggatct ttcggcggat agcacttcga aagcaagctg
    10201 gctgggttag ctttaaacag gccgttaccg acagcgatag tccccagggc gatgaaaatc
    10261 aggtcaggct taagtagcga catgccggtc atgaagtagc caatcgccag cacaagtgct
    10321 ccgagaacaa tggtgcgttt ggtccccagc aggtggtcgc cgacatagcc gccaatggaa
    10381 atgaggccat agaccagcgc agcaaaagca ccaaaagtga caaaagcctg ctcttgcgag
    10441 aatccaagct gtttaacgaa gaaaaccgcc agtacgccct gcacgccgta gtagccgaat
    10501 cgctcccata actcgacaaa aaagatcatg aaaaatgggc gaggttgctg cagcatcccc
    10561 atgggtgttg ttgtattcat atttattaac cttccaatac catcctgaaa agtatgaacg
    10621 cgtaagggat aacgcgcgaa aaagtttaca tctgtcagag attcttaata ttgatgcggt
    10681 gaatggatta atacactatt acaacagaaa ataaccagct aatcacacta atgatggaaa
    10741 atatgatggt gttaaatgtt gtattggcag gagattttga ttgcgtacgc agatcgtagg
    10801 ccagataagg ggtttacgct gcatcaggta acaaggcgcg ccaaaaagca aaacgcccgc
    10861 atatgcgggc gttcaggaac acgtcgtgga gggaagatta ttcttcttcg tcgcgcagcg
    10921 gaacaatcag catatcaacg tgaacggtgt tgatcagctg acgtgcggaa gacatcagtt
    10981 tgctccagaa gtcctggtgg tgaccacaaa ccaccagatc catatcgtat ttcttgattg
    11041 catcgaccag aacctggccc aggtcgccgc tgccgctcag ggtttcagtg attgggtagc
    11101 ctgcattagt ggaaagctcg gtcagcgcat gatgtgtctc ttcagagatg cgtttctgca
    11161 tatcacccag attcacatca ataagcccgg tgtataggtc agagtagttt acatctacgt
    11221 ggatcagaga aactttcgca ttgtaggggc gagccataga gactgctttc tctaccagaa
    11281 ctttgctttc cggggagaga tcgaccgcga tgagaatgtg tttataagcc atagtgttac
    11341 tccttccata aagttgtcga tgactggcca gctagcgttt cttgtgccgt tacgtcaccc
    11401 gcgtcctgcg aactacgtgc gcggggctaa cccagccgta aagattcagt gttaagacca
    11461 tccttacctt atagcgaccc ggatgatccg tcaatccgcc ttgcttaccc gttagataaa
    11521 caaattttcg acatgaatat attgatagtg gttaaccttc tggaaaaaaa acaacctgat
    11581 ctcctacact atctatagag ccgctcggat gttggtcacc acaaaagcgg tttctccagg
    11641 acctggtgtt aactgattgg cagcgtatcg ggagcggtag ccccggggtg gaactccgcg
    11701 ggcaggtcgc cggggaggag gtatgataag caccgtcgca ttattttggg ctttatgtgt
    11761 cgtttgcatt gttaacatgg cgcgctattt ctcatcacta cgcgcgttgt tagtggtact
    11821 gcgtaactgc gatccattgc tctatcaata tgttgatgga gggggctttt ttacctcaca
    11881 tggccaaccc aacaaacagg tgcgtctcgt ttggtatatc tatgcccaac gttatcgcga
    11941 tcatcacgat gatgagttta ttcgccgctg tgagcgggtg cgtcggcagt ttattctgac
    12001 tagcgcattg tgtggtctgg tggtggtcag cctgattgca ttgatgattt ggcattaagc
    12061 acttcagcaa taataacgca taaaaaaagc tggccagtaa actgacccgc tcgagactga
    12121 ttacaaacgc aaaattgcct gatgcgctac gcttatcagg cctgcataat ctctgcaatc
    12181 tattgaattt gcatgttttt gtaggccgaa taaggcgttc acgccgcatc aggcatgaac
    12241 aaagcgcact atgtcacaat ctgaagcggg ccagctgacc cgctcgtttt ggtgcgtacg
    12301 attacaggaa ctgcaaggag agccagtaca gcccgccgga aagcagtacc gcagcaggaa
    12361 gggtaaacac ccaggccatc agaatgctgg tcacggtttt acgctgtaag ccgccaccat
    12421 ctaccaccat cgtccccgcg acagaagagg agagtacgtg agtagtggaa accggcatcc
    12481 cggtataact cgccaggccg atagacactg ccgccgtcat ctgggcagac atcccctgag
    12541 cgtaggtcat gcctttctta ccgattttct caccgatagt cgttgccaca cgacgccagc
    12601 caatcatcgt accgatacct aacgccagcg cgaccgccat gatgatccac accggtgcat
    12661 actcgatggt gctaagcatg tcggacttca gtttcttcaa caggcgctga tcgtcagcac
    12721 tcacgccagg tatcttcact actttgtcga tagtgtcaga aacgcacagc ataatgcggc
    12781 gcatctggct acgttgatca agcgacagct tgtcgtagct ttccacatcg gtagttaaca
    12841 tacctttcag gcggttgagc gcgttaatgg tattcgacgg atggcaatgg aactccgcgg
    12901 gttgcgttgc gccagcttcc ggagccggta ctaactgatc agcaccggta gcctgtttca
    12961 gcagcgcagg atgctgctca aagtaagctt cgacgttgtt gatggcatca cgggtacggg
    13021 tgatttcgta gccagtggca ttcatattca ccacgaagcc tgctggtgcg acaccaatca
    13081 ataccaacat aaccagacca atgcctttct gaccatcgtt cgcgccgtgc gaaaacgcca
    13141 cgccgatagc ggaaaggatc agcgcaatac gcgtccagaa cggcggcttt ttcttgccgt
    13201 ctttcttttc acgctccgct ggggtcaggt ggatacgggc gcgtttcttg gtgccgctcc
    13261 agtagcgacg cagcaagaaa atcagaccgc cagcaaacac caggccgaca ataggggaaa
    13321 caatcagaga accgaaaata cttaatactt tcgggatatt gagtgcatcc accactgacg
    13381 tcccggtcat caacgcattg gttaaaccaa tcccgatgat cgcgccaatc agtgtatgag
    13441 agctggatgc aggtaaacca aagtaccagg tacccaggtt ccagataatc gccgccagca
    13501 acatagagaa caccatggca aggccatgag acgatcccat attaagcagc agatccgtcg
    13561 gcagcatatg cacaatggca taggcaacac tcagaccacc cagcaaaaca cccaaaaagt
    13621 tgaacaccgc cgccataacc acggcgagct gagaacgcat cgcgcgggta tagataacgg
    13681 ttgccacggc gttggctgtg tcatggaaac cattgatggc ttcgtagaac agcacaaaag
    13741 ccagtgcaag caataataac agcccggtat gcaaatccag gccagcaaac aaatgtagca
    13801 taggacgtta cgccattttg aggacatgaa cgcggcgcat tatcagtgac tttcgcggcg
    13861 cgggcaaagt gaaatataga ctttttttga tttgcctcct gtatggattt cactcaaaaa
    13921 ataattatct tatataattc aggcaaatac ttccttttag taatattgat gctggtgtga
    13981 ccactgagga atctttacaa ttcacgctcg ttttttctaa gaggagcgca acgtggaaag
    14041 gtttgatgcc attattatag gcgctggtgc ggcgggtatg ttctgttctg cgctggcagg
    14101 tcaggcagga cgccgggttc tgctgatcga taatggtaaa aaaccagggc gcaaaatcct
    14161 tatgtctggc ggtgggcgct gcaactttac caacctttat gtcgaaccag gcgcttatct
    14221 gagccagaat ccgcattttt gtaagtctgc actcgcgcgt tttacccagt gggatttcat
    14281 tgatctggtc aataaacacg gcatcgcctg gcacgagaaa acgttaggac aactcttctg
    14341 cgatgactcc gcgcagcaga ttgtcgacat gctggtggat gagtgcgaga agggcaatgt
    14401 aaccttcaga ttgcgtagcg aagtgctgag tgtggcgaag gatgaaacag gcttcacgct
    14461 tgaactgaac ggcatgactg tcggttgcga aaagctggtc atcgcgaccg gtgggctgtc
    14521 aatgccgggg ctgggcgcat cgccgtttgg ttataagatt gccgaacaat ttggcctcaa
    14581 cgtgctgccg acccgtgcgg gtctggtgcc attcactctg cataaaccgt tgctcgaaga
    14641 gttacaggtg ctggcgggcg tggcggtgcc ttccgtgatt accgctgaaa acggcaccgt
    14701 tttccgtgag aacttactct tcacccaccg cggcttatct ggaccggcgg tgttgcagat
    14761 ttccagctac tggcaaccgg gtgaatttgt cagtatcaat ctgctaccgg atgtggacct
    14821 cgaaaccttc ctgaatgagc agcgtaacgc acatccgaat cagagcctga aaaacacact
    14881 ggcggttcat ctaccgaagc ggttggttga acgcttacag caactcgggc aaatcccgga
    14941 tgtttcgcta aaacagctca acgtgcgtga ccaacaggca ctgattagca cattgaccga
    15001 ctggcgcgta caacccaacg gcactgaagg ctatcgcact gccgaagtga cgctcggcgg
    15061 cgtggacacc aacgaactct cttcacggac gatggaagcg cgcaaagtgc ctgggctgta
    15121 cttcatcggc gaagtgatgg acgttaccgg ctggctgggg ggctataact tccagtgggc
    15181 gtggtcgagt gcgtgggctt gtgcgcagga tttgattgca gcgaagtcgt cctgacctgt
    15241 tacctaaaga tgaaaattat tagagccgca ttaaaaatga gctaattttg atagtggcta
    15301 tcttgtgatt attttctaat gagcccgtga actgaaaccc tccaggctta atataaggtg
    15361 gaaggaaaga tgattgaaaa ctcacccagt ggccagatct ttataaaata tgaacaggct
    15421 gaaaaagaca gtaactttgt tgttttttat ccttaaccac tatgcattaa tgctgcgtta
    15481 tttcatgatg cctaagaaaa accagcgtta cgcaaatggt caacgctggt tttatccggt
    15541 acgttgcaat tattttttag cagaacctgc ttctaatatt gaaacgattg agaacaacgt
    15601 aaagcatacc gctcccaaac cgaccaaaac ccgcgaagga ataaaatatc ccatgtcggt
    15661 ctgcgccatt tccgcaagaa acgatgccag gaaaaggcaa aacaggcagg tgaagacggg
    15721 aatcatcggt attctgttgg ctaacgaaca ggtacgtctc cataccagtg ccagtagcca
    15781 gacttttgag aatatgctgt aacagatcat tccgaggtaa ataagaataa tgccgggagc
    15841 cagtcgggcg cttgcatcgg aactgactaa gacgtatatc ccctgcagta ccgtgattga
    15901 gccgagaaaa ataacccaat aacaccacag ccagtgttct ttagttgaaa acgtattacg
    15961 tgtttg
//
LOCUS       DAJTMC010000090        15959 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.090, whole genome shotgun sequence.
ACCESSION   DAJTMC010000090 DAJTMC010000000
VERSION     DAJTMC010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15959
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.090"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            6..1241
                     /locus_tag="OO130_002548"
     CDS             6..1241
                     /locus_tag="OO130_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR5365910.1"
                     /translation="MSKLPTGVEIRGKYIRIWFMFRGKRCRETLKGWEITNSNIKKAG
                     NLRALIVHEISSGEFEYLRRFPQSSTGAKMVTTRVIKTFGELCDIWTKIKETELTTNT
                     MKKTKSQLKTLRIIICESTPISHIRYSDILNYRNELLHGETLYLDNPRSNKKGRTVRT
                     VDSYIALLCSLLRFAYQSGFISTKPFEGVKKLQRNRIKPDPLSKTEFNALMESEKGQS
                     QNLWKFAVYSGLRHGELAALAWEDVDFEKGIVNVRRNLTILDMFGPPKTNAGIRTVTL
                     LQPALEALKEQYKLTGHHRKSEITFYHREYGRTEKQKLHFVFMPRVCNEKQKPYYSVS
                     SLGARWNAAVKRAGIRRRNPYHTRHTFACWLLTAGANPAFIASQMGHETAQMVYEIYG
                     MWIDDMNDEQIAMLNARLS"
     gene            1293..2228
                     /gene="ttcA"
                     /locus_tag="OO130_002549"
     CDS             1293..2228
                     /gene="ttcA"
                     /locus_tag="OO130_002549"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HCR5365911.1"
                     /translation="MQENQQITKKEQYSLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPVYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGIPHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(2357..3730)
                     /gene="dbpA"
                     /locus_tag="OO130_002550"
     CDS             complement(2357..3730)
                     /gene="dbpA"
                     /locus_tag="OO130_002550"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HCR5365912.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAVISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVPLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     CRVRLLK"
     gene            complement(4176..5159)
                     /gene="zntB"
                     /locus_tag="OO130_002551"
     CDS             complement(4176..5159)
                     /gene="zntB"
                     /locus_tag="OO130_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HCR5365913.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            5414..6646
                     /gene="dgcM"
                     /locus_tag="OO130_002552"
     CDS             5414..6646
                     /gene="dgcM"
                     /locus_tag="OO130_002552"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HCR5365914.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALSCRLVLRELTETEPVI
                     IFEGIEAPATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFVHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            complement(6667..7230)
                     /gene="smrA"
                     /locus_tag="OO130_002553"
     CDS             complement(6667..7230)
                     /gene="smrA"
                     /locus_tag="OO130_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HCR5365915.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMV
                     FSFIHQALADGLRNVLIIHGKGREDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(7560..8468)
                     /locus_tag="OO130_002554"
     CDS             complement(7560..8468)
                     /locus_tag="OO130_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5365916.1"
                     /translation="MAFQVKIHQIRAFVEVARQGSIRGASRMLNMSQPALSKSIQELE
                     EGLAAQLFFRRSKGVTLTDAGESFYQHASLILEELRAAQEDIRQRQGQLAGQINIGMG
                     ASISRSLMPAVISRFHQQHPQVKVRIMEGQLVSMINELRQGELDFTINTYYQGPYDHE
                     FTFEKLLEKQFAIFCRPGHPAIGARSIKQLLDYSWTMPTPHGSYYKQLSELLDDQAQT
                     PQVGVVCETFSACISLVAKSDFLSILPEEMGCDPLHGQGLVMLPVSEILPKAAYYLIQ
                     RRDSRQTPLTPSLITQFRRECGYLQS"
     gene            8644..9944
                     /gene="abgA"
                     /locus_tag="OO130_002555"
                     /pseudo
     CDS             8644..9944
                     /gene="abgA"
                     /locus_tag="OO130_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415854.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgA"
     gene            9944..11389
                     /gene="abgB"
                     /locus_tag="OO130_002556"
     CDS             9944..11389
                     /gene="abgB"
                     /locus_tag="OO130_002556"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgB"
                     /protein_id="HCR5365917.1"
                     /translation="MQEIYRFIDDAIEADRQRYTDIADQIWDHPETRFEEFWSAEHLA
                     SALESAGFTVTRNVGYIPNAFIASFGQGKPVIALLGEYDALAGLSQQAGCAQPTSVTP
                     GENGHGCGHNLLGTAAFAAAIAVKKWLEQYGQGGTVRFYGCPGEEGGSGKTFMVREGV
                     FDDVDAALTWHPEAFAGMFNTRTLANIQASWRFKGIAAHAANFPHLGRSALDAVTLMT
                     TGTNFLNEHIIEKARVHYAITNSGGISPNVVQAQAEVLYLIRAPEMTDVQHIYDRVAK
                     IAEGAALMTETTVECRFDKACSSYLPNRTLENAMYHALSHFGTPEWNSEELAFTKQIQ
                     ATLPPNDRQNSLNNIAATGGENGKAFALRHRETVLANEVAPYAATDNVLAASTDVGDV
                     SWKLPVAQCFSPCFAVGTPLHTWQLVSQGRTSIAHKGMLLAAKTMAATTLNLFIDSGL
                     LQECQQEHQQVTDTQPYHCPIPKNVTLSSLK"
     gene            11426..12952
                     /gene="abgT"
                     /locus_tag="OO130_002557"
                     /pseudo
     CDS             11426..12952
                     /gene="abgT"
                     /locus_tag="OO130_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate transporter"
     gene            12963..13478
                     /gene="ogt"
                     /locus_tag="OO130_002558"
     CDS             12963..13478
                     /gene="ogt"
                     /locus_tag="OO130_002558"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HCR5365918.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLSDYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            13673..14425
                     /gene="fnr"
                     /locus_tag="OO130_002559"
     CDS             13673..14425
                     /gene="fnr"
                     /locus_tag="OO130_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HCR5365919.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            14577..15527
                     /gene="uspE"
                     /locus_tag="OO130_002560"
     CDS             14577..15527
                     /gene="uspE"
                     /locus_tag="OO130_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HCR5365920.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFEPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(15577..15835)
                     /locus_tag="OO130_002561"
                     /pseudo
     CDS             complement(15577..15835)
                     /locus_tag="OO130_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
BASE COUNT         4134 a         3950 c         3914 g         3961 t
ORIGIN      
        1 atgatatgtc taaattacca acaggtgtcg agattcgagg taaatacatt cgcatctggt
       61 tcatgtttcg aggaaaacga tgtcgggaaa cattgaaagg ctgggagatt actaacagta
      121 acattaaaaa ggccgggaat ttaagagcgt tgatagttca tgaaatcagt tccggtgaat
      181 ttgagtattt aagacgtttt ccccagtcca gcactggggc aaaaatggtg acaacgaggg
      241 tcataaaaac attcggggag ctttgtgata tctggacaaa aattaaagag acagagttaa
      301 caacaaacac aatgaagaaa acgaaatcac aattaaaaac actcaggata ataatttgtg
      361 agagtacccc gatatcgcat attcgttata gcgatatctt aaactaccgg aatgaactgc
      421 tgcatggaga aacgctttac ctagataatc caagatccaa caaaaaagga agaaccgtgc
      481 gcacagttga tagctatatc gccctgctct gttcgttgtt acgttttgcg tatcagtcgg
      541 gatttatatc aaccaaacca tttgaaggag taaaaaaatt acagcgaaac agaataaaac
      601 ctgacccgtt atctaaaaca gaattcaatg cattaatgga aagtgaaaaa ggacagagcc
      661 aaaacttgtg gaaatttgcc gtttactccg ggcttcgtca cggggaactg gcagctctgg
      721 cgtgggagga tgtggatttc gagaagggaa ttgtgaatgt cagaagaaac ctgacgatac
      781 ttgatatgtt cggtccccca aaaacaaatg ccgggatccg gacggtaaca ttactgcagc
      841 cggctcttga agcactgaag gagcaataca aactgaccgg gcatcatcgc aaaagcgaaa
      901 tcacttttta tcatcgggag tacggcagaa ctgaaaagca aaaactgcat tttgttttca
      961 tgcccagggt gtgtaacgaa aaacagaaac cttattactc ggtaagcagt ttgggtgcaa
     1021 gatggaatgc agcagtaaaa cgtgctggta ttcgccgccg taatccgtac catacgcgac
     1081 atacttttgc ctgctggctg ttgacggcag gagcgaaccc ggcatttata gccagccaga
     1141 tggggcatga aactgcgcaa atggtgtatg aaatttacgg tatgtggatt gatgacatga
     1201 acgacgaaca gatagccatg ttgaatgcgc ggttatcgta gttgcaaagt ttgcccccaa
     1261 tttgccccat ttagtaccag agaactgaaa taatgcaaga aaatcaacaa attacaaaga
     1321 aagaacaata cagcctgaac aaattacaaa aacgtctacg tcgtaacgtg ggcgaagcca
     1381 ttgctgactt caatatgatt gaagaaggcg atcgcatcat ggtttgcctc tcagggggta
     1441 aagacagcta taccatgctg gagattctgc gcaatttgca gcaaagcgcg ccaatcaatt
     1501 tttcgctggt ggctgttaac ctcgatcaaa agcaaccggg cttcccagaa cacgttctgc
     1561 ccgtgtatct tgaaaagctg ggcgttgagt acaagattgt cgaagagaac acttacggta
     1621 tcgtgaaaga gaagattcca gagggcaaaa ccacttgctc actgtgttct cgccttcgtc
     1681 gcggtatcct ttatcgtacc gcaacggaac tgggggcgac gaagatcgcg ctgggtcacc
     1741 atcgtgacga tatcctgcag acgttgttct taaatatgtt ctacggcggc aagatgaaag
     1801 gtatgcctcc gaaactgatg agcgatgatg gcaaacatat cgttattcgt ccgctggcct
     1861 actgccgcga gaaagatatt cagcgatttg ccgatgcaaa agcgttcccg attattccgt
     1921 gcaacctgtg cggttcacag cctaacctgc aacgtcaggt gattgctgac atgttgcgtg
     1981 actgggataa acgttatcca gggcgtatcg agacgatgtt cagcgcgatg cagaatgtgg
     2041 tgccgtcgca tctgtgcgat accaacctgt tcgatttcaa aggcatcccc cacggttctg
     2101 aagtggttaa cgggggtgat ctggcgtttg atcgcgaaga gatcccacta caaccggcgg
     2161 gctggcagcc agaagaagat gaaaatcagt tggatgagtt acggctgaat gtggttgaag
     2221 tgaaataacc aggatagcgc ccgatgcgca agcgtatcgg gctactctta tggaggccgg
     2281 ataagacgcg gccaacgtcg catccggcaa tgccgaagaa gatgtttact cttgcacccg
     2341 gcaattcaac atttcattat tttaataacc gcacccggca cgtttttcct ttaatcttcc
     2401 cgccctgtaa ctgtttccat gctttatgag caacagcctg acggaccgcg acatagacat
     2461 gcgccggatg cacggcgatt ttgccaatat ctgcgccatc aagcccgata tctcctgtca
     2521 gtgcacctaa tacatcaccc gggcgcattt tggctttttt cccgccatcg atacacaacg
     2581 ttgccatttc tgcttccagc ggcacaatgg aactattagc tggcggcgtt tgccagttaa
     2641 gttttatctg caacatgtca gaaatgatat tggcccgctg tgcttcttcc ggagcacaga
     2701 aactgatcgc cagaccgcta tttcctgcac gagctgtacg gccgatgcga tgtacatgaa
     2761 cttcagggtc ccacgccagc tcaaagttca ccaccagctc aagcgattta atatccagac
     2821 cacgcgcagc aacatcagtc gcaaccagta cacgggcgct accgttagca aaacgtacca
     2881 gggtctgatc gcgatcgcgt tgctccaaat cgccgtgtaa tgacaatgca ctttgcccta
     2941 cttcattcag cgcgtcgcag acagcctggc aatctttttt ggtattgcaa aacaccacgc
     3001 aagaggatgg ctgatgcaag cttaataacc gttgcaacag aggaattttg cctttgctgg
     3061 atgtctcata aaattgttgt tcaatgggtg gcaaagcatc tgttgagtca atttcaatcg
     3121 ccaaaggatc gcgttgcact cgtccgctga ttacggcgat ggcttccggc caggttgccg
     3181 aaaacagaag cgtctgtcga gatgcaggcg caaaacggat gacatcatca atggcatcgc
     3241 taaatcccat atccagcatg cggtcggcct catccatcac caaagtattc agcgcatcca
     3301 gtgataccgt gcctttttgc aagtgatcca gcaaacgacc cggcgttgcc acgataatat
     3361 gcggcgcatg ttgcaacgaa tcacgctgca taccgaacgg ttgaccaccg cacaacgtca
     3421 aaattttggt atttggcaga aaacgcgcca gccgacgcaa ttcacctgcc acctgatccg
     3481 ccagttcacg cgtaggacac agcactaaag cctgggtttg aaatagcgac gcatcaattt
     3541 gctgtaacaa gccgaggcca aaagccgccg ttttgccgct gccggttttc gcctgcacgc
     3601 gaacatcttt tccggcaagg atcgccggaa gcgcggcggc ctgcaccggc gtcatagtta
     3661 aataacccaa ctcattaagg ttcgtgagtt gggcgggagg caaaacattc agggtagaaa
     3721 aagcggtcac aatctattct cgtggtcatc gacgcaaagt tagcaggcgc gtatcctcgc
     3781 agatctacgc gggtggtggg tcaggcatgg gttgcgggcg agggatcgga tcgggcactg
     3841 gaacaggatc gccaggaatc ggttcaggga caggaatttg caaataaata agtgtcgtca
     3901 tatttccctc cggtcattgg gtggactctt aaagggtaga cgctgataaa taacaggcaa
     3961 aaaaaagccg actcatcaaa gtcggcgtcg tacgaatcaa ttgtgctatg cagtaattca
     4021 aaaaaggaag taagacaata tggagcgcaa cgcccatcgc ttgacgttgc attcacctgc
     4081 aagggagata ttgccctgaa tgggtagaga gtttattgac ttcgctcaaa ctttgcggcg
     4141 tttttgtata cagacagccg gaaaaattgc ttttgttaca accatttact acgatgcaac
     4201 cataaagcaa caccaccaat aagaacaact aacagaatac aaaaaattga aaatccgaat
     4261 tgccacccgc cgccagggat cccaccaagg ttgacgccaa ataaccctgt cagaaaggta
     4321 ctgggtaaaa agaccattgc catcaatgac attgtatagg tacgacgagc taaattttcc
     4381 tgcatcacct gagcgatttc atccgccatc acgccagtcc gtgctataca ggcgtcgatt
     4441 tcgtcaagac cgcgcccaag gcgatcggca atatcctgca tccgacggcg ttggtcatcg
     4501 ctcatccacg gcaaacgttc actggcaaga cgagcataaa catcacgttg cggtgccata
     4561 tagcgacgca tcacgattaa ttgtttgcgc agcagagcca ggaatccacg cggtggaatt
     4621 tgctgatcaa ggagattatc ttcaaggtcg ataattttat cgtgcagctg ctcgataaat
     4681 tcgctggaat gatcggtcaa cgcatcgcac acatccacca gccaccctcc gcaatcggtc
     4741 ggacccgtgc cctcttccag atcgctcacc acatcgtcca gcgccagcac tttgcgttgt
     4801 cgggtcgaaa caattaaccg cccgtccata tatacacgca tggcgaccag ttgatcgggg
     4861 cgttcatcgg tgctgccgtt tatacagcgc aatgtaatca gcgtgccttc accgagacgg
     4921 ctgactcggg gccgtgtgct ctcgcctgcc agcgcatcac gtacgttatt gggaagcagc
     4981 ggtgttgtcg ccagccattg ggcgctatca tggtgtacat aattaaggtg gagccagcag
     5041 ggatgcgctt catcaatcac atctgtattt tccagcggtt taacgccgcc tctaccatcc
     5101 agcatccagg caaatactgc atccgggaca ttaacgtccg atcccttaat cgcttccaca
     5161 gtgcctccat catcaacgca ttattttgta gtctagcctt ctggccctgt tacgcaacat
     5221 ctcatcaccc cattaccctg aaatgattaa taaaattctg tctaaattga atacaaaaag
     5281 caaaatgctt ttccgtatac aaaccgtgtg aagtgttaaa tagcgtctat cattatcaga
     5341 attatctgat catatgacgt ggcttttttg cgatcggata gcaacaaaaa ttgataaaaa
     5401 taacgggatc tcaatgatta cgcacaactt caataccctg gacttactca ccagtcctgt
     5461 ctggattgtt tcgccctttg aggaacagtt aatttatgcc aatagcgcgg cgcgactgtt
     5521 gatgcaagac ctcacgttta gtcagctacg aaccggaccc tattccgtct cctcacaaaa
     5581 agaactgccg aaatacctct ccgatctgca aaaccaacac gatattatcg aaatcctcac
     5641 tgttcagcgt aaagaagagg aaacagcatt aagctgtcgg cttgttttgc gagagctgac
     5701 agaaacagaa ccggtgatta ttttcgaagg tatcgaagcg ccggcaacgc tgggtttaaa
     5761 agccagtcgc tcggcaaatt atcagcgcaa aaaacaaggt ttttatgcgc gcttttttct
     5821 gactaactct gcaccaatgt tgttgattga cccctcacga gatggccaaa tcgtcgatgc
     5881 taacctcgcc gcgctcaatt tctacggtta taaccatgaa acgatgtgcc agaaacatac
     5941 ctgggaaatt aatatgctcg ggcgtcgcgt catgcctatc atgcatgaaa tttcgcattt
     6001 acccggcggt cataaacctt tgaattttgt tcataaactg gcggatggat ctactcgtca
     6061 tgtgcagacc tatgccgggc cgattgaaat ttatggcgac aagctcatgt tatgtattgt
     6121 gcatgatatt actgagcaaa aacggctgga ggagcagctg gaacatgctg ctcaccatga
     6181 cgcgatgacc ggattactga atcggcgaca gttttatcac attacggaac caggccaaat
     6241 gcagcacctc gccatcgccc aggattacag cttgttgctc atcgacaccg atcgttttaa
     6301 acacattaac gatctctatg ggcattctaa aggtgatgag gtgttatgcg ccctcgcccg
     6361 caccctcgaa agttgcgctc gcaaaggcga tttggtgttt cgttggggag gcgaagagtt
     6421 tgtcttattg ctaccaagaa ccccactgga taccgcgctt tcgctggctg aaactatccg
     6481 tgtaagcgtg gccaaagtga gtatttcggg cttaccacgt tttaccgtca gcattggtgt
     6541 ggcgcatcac gaaggaaatg aaagcatcga tgaactgttt aaacgcgttg atgatgcttt
     6601 gtatcgggcg aaaaatgatg gacgcaaccg cgtgctggcg gcataagccg cggatgcgtc
     6661 tcgagatcaa cggctgcgct tagcgtggcg ctcccagttt tcttgcttcg cctgcgccgt
     6721 tttacgtagt gcgacgtaac acgccccgct gccgccatga tgcggtagcg cggtgcaata
     6781 tgcctgaaca tcatcaaatt cggtcagcca gcgcgccaca tagctgcgga caatattggc
     6841 atgcgattta tcttcccgcc ctttaccatg aataatcagc acattacgca aaccatccgc
     6901 cagggcttgg tgaatgaaac tgaacaccat tttgcggcac tcttccaccg gctggcgcaa
     6961 aagattcagg ctcgcctgtt gcggatattt accactgcgc agcttatcca gcaccccatg
     7021 ttgcaaccct tcccgccgaa actccagcgg ctgacttagc gggatgatgt cgagaaatcc
     7081 ggtggtgagg aaattatcaa gctgcagcgt gtcgatacgc tgcggcgcac gttggttacg
     7141 cgttggatgc cagtggacat ctgtagcacg tttcagcggc tggacatctt ccatggcgtc
     7201 aagaaacagc gatttgtcgt caaggttcat gttacatcct cccgcaatta agagcgcgat
     7261 atgataacca gaccgggtcg gtccaacaac gtattaccaa aaattccagt aataagttcc
     7321 aaatattgcc gatattttaa gcaaaatgct tatgcatgat tattcattca cgatattaat
     7381 aatgtaactt atattttcgt gaaatctgtc actgaagaaa attggcaact aaaggttaaa
     7441 accgttataa cacagtcacc ggcgcagagg agacaatgcc ggacttaaga cgcggatgca
     7501 ctgctgtgtg tactgtagag tctggcggat gtcgacagac tctatttttt tatgcagttt
     7561 taactttgca gatagccgca ttctcgccgg aattgcgtga ttaatgacgg ggtcagtggt
     7621 gtctggcgac tatcacgccg ctgaatcaaa taataggccg ctttcggtaa aatttcgcta
     7681 accggcaaca tcaccagccc ctgtccgtgc aagggatcgc agcccatttc ttcaggcagt
     7741 atgctgagaa aatcgctttt tgccaccaga ctgatacagg ctgagaacgt ctcgcagact
     7801 acaccgacct gtggcgtttg cgcctgatcg tcaagcaatt cactcaactg tttgtagtaa
     7861 ctgccgtgtg gcgtcggcat tgtccagctg taatccagta actgtttgat cgaacgggca
     7921 ccaatggcgg ggtgtcccgg gcggcaaaag atcgcgaatt gcttttccag taatttctca
     7981 aaagtaaatt cgtggtcgta cggtccctga taataggtat tgatggtgaa atccaattct
     8041 ccctgacgca attcattaat catcgacacc agttgccctt ccataatgcg tacttttacc
     8101 tgcggatgct gctgatgaaa acgagatatg acagctggca tcagactgcg ggaaatactg
     8161 gcccccatgc cgatattaat ctgccctgcc agttgccctt gtcgttggcg aatatcctct
     8221 tgggctgcgc gcagctcttc aagaataaga ctggcgtgct gataaaaact ttcaccggca
     8281 tcagttaacg tcacgccttt actacggcga aaaaagagtt gcgccgctaa tccttcttcc
     8341 aactcctgaa tagatttact cagtgccggt tgcgacatat tcaacattcg gctcgctccg
     8401 cgaatgctgc cctgacgagc cacttcaaca aaagcacgaa tttgatgaat ttttacctga
     8461 aaagccatga cgccaccgat aaccgttatt tatcagacca aagaaactgg catctacttt
     8521 aatgcagatg attgtgtcag ggtaatttat gaacggttaa aactgtgaaa aatcagttag
     8581 tgataagtaa aaactatcgc tacgtgaacc gggtcacact ttttactgat gacgggaaag
     8641 gttatggagt ctttgaatca atttgttaat tcgcttgccc caaaattatc gcactggcga
     8701 cgtgattttc atcactatgc agagtctggc tgggtggaat tccgcactgc cacccttgtt
     8761 gcggaagaat tgcagcagct cggctattca ctggcgctgg gccgcgaagt agttaatgaa
     8821 agtagccgga tgggactacc tgatgaactc actctacaac gcgaattcga gcgcgctcgt
     8881 caacagggtg cgctagcaca atggattgcg gtttttgaag gtggtttcac tggtatcgtc
     8941 gccaccctgg ataccggtcg ccccggtccg gtgatggctt tccgtgtcga tatggacgcg
     9001 ctggatctca gtgaagagca ggatgtcagc catcgcccct accgcgacgg ttttgcgtca
     9061 tgtaacgccg gaatgatgca tgcctgtggt catgatggac ataccgccat tgggcttggg
     9121 ctagcgcata cccttaaaca attcgagtcc ggactacatg gcgtcatcaa actgattttt
     9181 cagcctgcag aggaaggtac gcgtggcgcg cgggcgatgg tcgatgcagg tgtcgtagat
     9241 gatgttgatt attttactgc cgtgcacatt ggcactggcg tacctgcggg caccgtggtg
     9301 tgcggcagtg ataattttat ggcaaccacc aaatttgacg cgcacttcac cggtaccgcc
     9361 gctcacgcag gcgcaaaacc agaagacggt cacaatgcct tgttggcggc agcacaagcc
     9421 actcttgcac tgcatgcaat cgccccggag cttccagagt aaacgtgggc gttatgcagg
     9481 caggaagcgg tcgtaacgtt gttcctgcct cggcgttgct gaaagtggaa acacgcgggg
     9541 ccagcgacgt cattaatcaa tatgtttttg accgtgcaca acaagcgatt cagggcgcag
     9601 caaccatgta tggtgtcggc gttgaaactc gtctgatggg tgcagctacc gccagttctc
     9661 cttcgccgca atgggtcgca tggttgcaaa gtcaggcggc tcaggtcgcg gggggcaatc
     9721 aggccattga acgtgttgaa gcgcctgcgg gttccgaaga tgccacatta atgatggccc
     9781 gcgtgcagca acatcaaggg caagcctcct acgtggtgtt tggcacacag ctggcggcag
     9841 gtcatcacaa cgaaaaattc gattttgacg agcaggttct cgctattgcc gtcgaaacgc
     9901 tggcgcgcac cgcgctcaat tttccctgga cgcgaggtat ctgatgcagg aaatctatcg
     9961 ttttatcgac gatgcgattg aagccgatcg ccaacgttat accgatattg ccgatcaaat
    10021 ctgggatcat ccagaaacac gttttgaaga gttctggtca gcggagcatc tggcttcggc
    10081 gctggaatct gcaggcttca ccgttacccg caacgtaggc tatatcccaa atgcctttat
    10141 tgcttcgttt ggtcaaggca aaccggttat cgccctgctg ggagaatatg acgccctggc
    10201 aggtttaagt cagcaagcag gttgcgcgca acctacatcc gtgacgcccg gtgaaaatgg
    10261 tcacggttgc ggacacaatt tgctgggaac cgccgccttt gccgctgcaa tagccgtcaa
    10321 gaaatggctg gaacaatatg ggcaaggcgg cacggtgcgc ttttatggtt gtcctggcga
    10381 agaaggcggc tcgggtaaaa cgttcatggt tcgcgagggg gtatttgatg atgtggatgc
    10441 ggcactcact tggcacccgg aagcctttgc cggtatgttc aatacccgca cgctggcaaa
    10501 cattcaggca tcatggcgct ttaaagggat cgcagcacat gccgcgaatt tccctcattt
    10561 gggacgcagc gcccttgatg ccgtaacgtt gatgaccact ggcaccaact tcctcaacga
    10621 acatattatt gaaaaagcgc gcgtacacta tgccatcaca aatagcggcg ggatctcgcc
    10681 caacgtggtc caggcgcagg cagaagtgct ttatcttatc cgcgcccccg aaatgaccga
    10741 cgtgcagcat atttatgatc gggtcgccaa aatcgccgaa ggtgcggcat tgatgaccga
    10801 aaccacggtt gaatgccgct tcgacaaagc ctgttccagt tatctcccga atcgcacctt
    10861 agaaaatgcc atgtaccatg ccctatccca ttttggtacc ccggaatgga actccgaaga
    10921 actggctttt acgaaacaaa ttcaggctac gctccccccc aacgatcggc aaaacagtct
    10981 gaataatatc gccgcaaccg gtggcgaaaa cggcaaggct tttgcactac gtcatcgtga
    11041 aacggtactg gcgaatgagg tcgctccata tgccgccacc gataacgtgc ttgcggcatc
    11101 gactgatgtc ggcgacgtca gttggaaact gcctgttgcc cagtgtttca gcccctgttt
    11161 tgccgtcggt acaccgctac atacgtggca actggttagc caggggcgaa catctattgc
    11221 tcataaagga atgctgctgg cggcgaaaac tatggcagca accacactca atctcttcat
    11281 tgattcaggg ctattgcaag aatgccaaca agagcatcag caagttacgg acacgcaacc
    11341 gtatcactgc cctatcccaa aaaacgtgac actgtcatct ttaaaataac aacaacaacg
    11401 caaacacaac aaccgaggaa tgcccatgag tatgtcatcc ataccgtcgt cctcctaatc
    11461 cgggaagctc tatggctggg tcgaaagaat tggtaacaag gttccccatc cttttttgct
    11521 ctttatctat ttgattatcg tactcatggt gacgacggca attttgtcgg cctttggcgt
    11581 cagtgcgaaa aacccgaccg atggaacgcc ggtggtggtg aaaaacctgc tcagtgtgga
    11641 aggattacac tggtttttac ccaatgttat taaaaacttt agcggttttg ctccacttgg
    11701 tgcgatcctg gcgctggttt taggtgccgg tctggcggag cgcgtcggct tactgccagc
    11761 gctaatggtt aaaatggcat cgcatgttaa tgtccgctac gccagttata tggtgctgtt
    11821 tattgctttt ttcagccaca tttcttccga tgcggcgtta gtgatcatgc caccgatggg
    11881 tgcgctgatt tttctggcgg tgggcaggca tccagttgca ggtttactgg ctgccattgc
    11941 aggcgtaggt tacggcttta cggctaattt actgattgtc acaaccgacg tgttgctgtc
    12001 ggggatccgc acggaagcgg cggctgcgtt caatccgcaa atgcacgtca gtgtaattga
    12061 taactggtat tttatggcca gctccgtagt cgtactgacg attgttggcg gcctgataac
    12121 cgacaaaatc atcgagccac ggttaggtca atggcaggga aacagcgatg agaaactgca
    12181 gacattgacc gaaagtcagc gttttggttt acgcatagca ggtgtcgtat cgctactttt
    12241 tattgctgcg atcgcgctga tggtgatccc ggaaaacggg atattgcgcg atccgattaa
    12301 tcacaccgtg atgccatcac cctttattaa aggtatcgtg ccactaatca ttcttttttt
    12361 ctttgtggtt tcgctggctt atggcatcgc tacccgcaca attcgacatc aggcggattt
    12421 accgcattta atgattgaac cgatgaaaga gatggcggga tttatcgtga tggtttttcc
    12481 cctcgcccag tttgtcgcca tgtttaactg gagcaacatg gggaaattca tcgccgtggg
    12541 gctgaccgat atactggaaa gttcagggct tagcggcatc ccggcgtttg tcggtctggc
    12601 gttgctttcc tctttcttat gcatgtttat tgccagtggt tccgcaatct ggtcgattct
    12661 ggcccccatt ttcgtaccaa tgtttatgct acttggcttt cacccggcat ttgcgcaaat
    12721 cctctttcgt attgccgact catccgtatt gcctttagcg ccggtatctc cttttgttcc
    12781 actgtttctt ggattcctgc aacgctacaa accagacgcg aaactgggta cttactattc
    12841 gttagtcttg ccctatccgc ttatcttttt ggtggtatgg ctgctgatgt tgctggcgtg
    12901 gtatcttgtt ggtctgccga taggtccggg gatttaccca cgtttgtctt aagagagaac
    12961 ggatgctgag attacttgaa gaaaaaattg ccacgccact gggtccactg tgggtgattt
    13021 gcgatgagca atttcgcctg cgggcggttg aatgggaaga gtacagcgaa cgcatggtgc
    13081 agctgctgga catccattat cgcaaagaag gctatgagcg catttctgcc accaacccag
    13141 gcggtttaag cgacaagctt agtgattatt ttgccggtaa tcttagcatt attgatacgc
    13201 ttcccactgc cacagggggg acgccatttc agcgcgaagt ctggaaaact ctacgcacta
    13261 tcccctgcgg gcaggtaatg cattacggcc aactggctga acaattgggc cgccctggcg
    13321 cggcgcgtgc cgttggtgcg gcaaacggat cgaatcccat cagcatcgtc gtaccttgcc
    13381 atcgggttat tggccgaaac ggcaccatga ccggatatgc aggcggagtt cagcgaaaag
    13441 agtggttatt gcgccacgaa ggttatcttt tgctgtaaac attaaacaat ttgtgccagc
    13501 ttgttcacac ttttatgtaa agttaccctt aacaacttaa tggttttcaa atagatagac
    13561 atatatttac atctaatatc ggaattctct gctgttaagg tttgcttaga cttacttgct
    13621 ccctaaaaag atgttaaaat tgacaaatat caattacggc ttgagcagac ctatgatccc
    13681 ggaaaagcga attatacggc gcattcagtc tggcggttgt gctatccatt gccaggattg
    13741 cagcatcagc cagctttgca tcccgttcac actcaacgaa catgagcttg atcagcttga
    13801 taatatcatt gagcggaaga agcctattca gaaaggccag acgctgttta aggctggtga
    13861 tgaacttaaa tcgctttatg ccatccgctc cggtacgatt aaaagttata ccatcactga
    13921 gcaaggcgac gagcaaatca ctggtttcca tttagctggc gacctggtgg gatttgacgc
    13981 catcggcagc ggccatcacc cgagcttcgc tcaggcgctg gaaacctcga tggtatgtga
    14041 aatcccgttc gaaacgctgg acgatttgtc cggtaaaatg ccgaatctgc gtcagcagat
    14101 gatgcgtctg atgagcggtg aaatcaaagg cgatcaggac atgatcctgc tgttgtcgaa
    14161 gaaaaatgcc gaggaacgtc tggctgcatt catctacaac ctgtcccgtc gttttgccca
    14221 acgcggcttc tcccctcgtg aattccgcct gacgatgact cgtggcgata tcggtaacta
    14281 tctgggcctg acggtagaaa ccatcagccg tctgctaggt cgcttccaga aaagcggtat
    14341 gctggcagtc aaaggtaaat acatcaccat cgaaaataac gatgcgctgg cccagcttgc
    14401 tggtcatacg cgtaatgttg cctgattttt ccgcataact cactatcctt ctgtcatatc
    14461 attaaatttt tctgatttat tgatctggca gaaggttcat cactgtttca ttcaccagat
    14521 atgggttaat cttttaatta caaactgcgt tgacagttgt tgtaaggaga ccctgtatgg
    14581 ctatgtatca gaacatgctc gttgttatcg atcctaacca ggacgaccaa ccagcattgc
    14641 ggcgagctgt ttatttacat caacggattg gtggcaaaat taaagccttt ttgccgatct
    14701 atgacttctc atacgaaatg accaccctgc tctccccgga cgaacgtacc gctatgcgtc
    14761 agggcgtcat cagccagcgt acagcctgga tccacgagca ggcaaaatat tatctcaatg
    14821 ctggcgttcc cattgaaatt aaagtggtct ggcataaccg tcctttcgaa gccatcattc
    14881 aggaagtgat cagcggcggg cacgatttgg tgctaaaaat ggcgcaccaa catgaccgtc
    14941 tggaagcggt gatttttacg ccaacggact ggcatctgtt acgcaaatgc ccaagcccgg
    15001 tgtggatggt gaaagaccag ccgtggccgg aaggaggtaa ggcgctggtg gcggtgaatc
    15061 tcgccagtga agagccgtat cataatgcgc tcaatgaaaa actggtcaaa gagacgatcg
    15121 aactggcaga acaagtcaac cataccgaag ttcatctggt tggcgcttac ccagtaacgc
    15181 caattaatat cgcgattgaa ctgccggaat ttgagccgag cgtttataac gatgccattc
    15241 gtgggcaaca tttgctggca atgaaagccc tgcggcagaa attcggcatt aatgaaaaca
    15301 tgacgcacgt agaaaaaggt ctgcccgaag aggttattcc tgatttggcg gagcacttac
    15361 aggcggggat tgtggttctg ggcacggtag gacgcaccgg tatttcagca gcattcctcg
    15421 gcaacacggc ggaacaggtg attgatcatc ttcgctgcga tctgttggtc attaaacctg
    15481 accagtatca gacacccgtt gaactggatg acgaagaaga cgattaacga ctcttcaata
    15541 actgacaagg cccgagttat cgggcctatt ttttacttac tgctcttcgc cgccaaggaa
    15601 ataaattccc aacgggattg ccagcaacac agtaaagacc aggctataaa caaaaatcat
    15661 cgatgactga atcacataca ttgatgtcgt ccactcagac agcggaatat tgtactgttc
    15721 tataacgccg ccgatagtcg cgcgagtcac aaccgacgcc gcaataatga caaaccgcca
    15781 acaaaccaaa gagaaatttc ttccctttcg ctgacttcag tttcgccata atcatgatgt
    15841 aatccttgtg cagacaaatt tcttgttacc taaacgtaac aataccatga catttcaccg
    15901 ttgtctgctc tgaaaacctc acctcttagc tggatttaca atcaacgcat tgattagtt
//
LOCUS       DAJTMC010000091        15689 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.091, whole genome shotgun sequence.
ACCESSION   DAJTMC010000091 DAJTMC010000000
VERSION     DAJTMC010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15689
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.091"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            131..379
                     /locus_tag="OO130_002562"
     CDS             131..379
                     /locus_tag="OO130_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HCR5365921.1"
                     /translation="MARLILLTEWAKEEFSEPVPTPSTLSKYAKAGMIFPLPKKVGRR
                     WRVDPQARFVGMVNKPEVIATDHPALKRILEDGAPAKI"
     gene            357..1487
                     /locus_tag="OO130_002563"
     CDS             357..1487
                     /locus_tag="OO130_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HCR5365922.1"
                     /translation="MARPRKYKTDVPGLSPYFDKRNNKVYWRYRHPITGKNHGLGSID
                     QKLAETIAAEANSRLARQQMEQMLSLQEKIISDTGGSSTVTIFLNNYRKIQQERYENG
                     EIKLNTLKQKAAPLRVFDERFGTRRLDAITVKDVVSVLEEYKARGHNRMGQIFRKVLI
                     DVFREAQQTGDVPPGFNPAESAKKPQVRISRQRLTFDEWMMIYNAAEKDGYFLQRGML
                     LALMTGQRLSDICKMQFSDIRDGYLHVEQQKTGTRIAIPLALRCDKLNLTLDDVVSSC
                     RDCVLSPWLLHHHHAKGTAKRGGMVKPATLTVAFKKARDSVDYNWRANGTPPSFHEQR
                     SLSERLFREQGVDTKILLGHSNQKMTDIYNDARGKEWKKLVI"
     gene            complement(1533..2171)
                     /gene="ompW"
                     /locus_tag="OO130_002564"
     CDS             complement(1533..2171)
                     /gene="ompW"
                     /locus_tag="OO130_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HCR5365923.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTAKYKSGVTTVKDSVRLDPWVFMFSAGYRF"
     gene            2411..2500
                     /locus_tag="OO130_002565"
                     /pseudo
     CDS             2411..2500
                     /locus_tag="OO130_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            2528..3271
                     /locus_tag="OO130_002566"
     CDS             2528..3271
                     /locus_tag="OO130_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HCR5365924.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDSVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRISALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAVLLIYLFRLYMLIRQ"
     gene            3301..3840
                     /locus_tag="OO130_002567"
     CDS             3301..3840
                     /locus_tag="OO130_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HCR5365925.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            3945..4343
                     /gene="yciA"
                     /locus_tag="OO130_002568"
     CDS             3945..4343
                     /gene="yciA"
                     /locus_tag="OO130_002568"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HCR5365926.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(4383..5111)
                     /gene="tonB"
                     /locus_tag="OO130_002569"
     CDS             complement(4383..5111)
                     /gene="tonB"
                     /locus_tag="OO130_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HCR5365927.1"
                     /translation="MTSMTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAP
                     AQPISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPK
                     PKPVKKVQEQPKRDVKPVESRPASPFENTALARLTSSTATAATSKPVTSVASGPRALS
                     RNQPQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRY
                     EPGKPGSGIVVNILFKINGTTEIQ"
     gene            5326..5622
                     /locus_tag="OO130_002570"
     CDS             5326..5622
                     /locus_tag="OO130_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HCR5365928.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            5922..7175
                     /gene="kch"
                     /locus_tag="OO130_002571"
     CDS             5922..7175
                     /gene="kch"
                     /locus_tag="OO130_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HCR5365929.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCLAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
     gene            complement(7230..7403)
                     /locus_tag="OO130_002572"
     CDS             complement(7230..7403)
                     /locus_tag="OO130_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HCR5365930.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            7546..9006
                     /gene="cls"
                     /locus_tag="OO130_002573"
     CDS             7546..9006
                     /gene="cls"
                     /locus_tag="OO130_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCR5365931.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEIVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            9041..9370
                     /locus_tag="OO130_002574"
     CDS             9041..9370
                     /locus_tag="OO130_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HCR5365932.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPVEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(9423..10427)
                     /gene="oppF"
                     /locus_tag="OO130_002575"
     CDS             complement(9423..10427)
                     /gene="oppF"
                     /locus_tag="OO130_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HCR5365933.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGIKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(10424..11437)
                     /gene="oppD"
                     /locus_tag="OO130_002576"
     CDS             complement(10424..11437)
                     /gene="oppD"
                     /locus_tag="OO130_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HCR5365934.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTTVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPEHELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTLGRLCACFKPVE
                     ELL"
     gene            complement(11449..12357)
                     /gene="oppC"
                     /locus_tag="OO130_002577"
     CDS             complement(11449..12357)
                     /gene="oppC"
                     /locus_tag="OO130_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HCR5365935.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFIHNRAAVASLM
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTSGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            complement(12372..13292)
                     /gene="oppB"
                     /locus_tag="OO130_002578"
     CDS             complement(12372..13292)
                     /gene="oppB"
                     /locus_tag="OO130_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HCR5365936.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            complement(13378..15009)
                     /gene="oppA"
                     /locus_tag="OO130_002579"
     CDS             complement(13378..15009)
                     /gene="oppA"
                     /locus_tag="OO130_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HCR5365937.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            15300..15596
                     /locus_tag="OO130_002580"
     CDS             15300..15596
                     /locus_tag="OO130_002580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5365938.1"
                     /translation="MRFLLANTFCKIVKQFSFIDRIRIYSLHPCLNKHLSIQSNRDWL
                     SMSIFSDYSFSIEMHNNLTIDYYLALSSTKGSGITNIISIILQQAQDYDVAKIT"
BASE COUNT         4039 a         3799 c         3681 g         4170 t
ORIGIN      
        1 cgttaccagg caaaatacat ttcagttatc tggcaaaaac tgatcccgaa tcaggctgat
       61 ttttaatgtt caaccccggt cgttgcccac cagctatagt ggcggcgacc atgattagcg
      121 aacgacgctc atggcaagac ttattctgct cactgagtgg gcaaaagagg aattcagtga
      181 accggtccct actccgagta cgttaagtaa atacgctaaa gccggaatga tatttcctct
      241 ccccaaaaaa gttggaagac gctggcgagt ggatccgcaa gctcgctttg tcggaatggt
      301 aaacaagccg gaggtgatcg ccacagatca ccctgctttg aagaggatac tggaagatgg
      361 cgcgcccgcg aaaatataaa accgatgttc cgggattatc tccgtatttt gacaaaagaa
      421 ataacaaagt ttactggcgt tacaggcatc ccataacagg caaaaatcac ggtctcggca
      481 gtattgacca gaaactggca gaaactattg cagcagaagc gaacagccgt cttgcccggc
      541 agcaaatgga acaaatgctc agtctgcagg agaaaattat tagtgatacc ggcggttcat
      601 caactgttac catttttctg aataattaca gaaaaattca acaggaaaga tatgaaaacg
      661 gcgagatcaa actcaacacg ctgaaacaga aagcggcccc tctcagggta tttgatgaac
      721 gttttggcac cagacggtta gatgccataa ccgtaaaaga tgtggtatca gtactggaag
      781 agtacaaggc cagaggacat aacagaatgg gacaaatttt caggaaagta ctgatcgatg
      841 ttttccggga agctcagcaa acgggcgatg tcccgccagg ctttaaccct gcagaatcgg
      901 caaaaaaacc gcaggtgcgg atatcaagac agcgactgac ttttgatgag tggatgatga
      961 tttataacgc agcggaaaag gatggttact ttttacagcg cggtatgctg ctggcactga
     1021 tgacaggcca gcgcctttca gatatttgca aaatgcaatt ttcggatatc cgggatggtt
     1081 atcttcatgt cgaacagcaa aaaacaggaa cccggattgc catccctctg gctctgcgtt
     1141 gcgataaatt aaatctcacc ctggatgatg tggtgtcatc ctgccgcgat tgcgttctta
     1201 gtccgtggct attgcaccac catcacgcga aagggacagc taagcgcggc gggatggtta
     1261 agccagcaac attaaccgtt gcatttaaaa aagcccggga ttctgtggat tacaactggc
     1321 gtgctaatgg cacccctccc tctttccatg agcagagatc tttatcagag cgattgttca
     1381 gagagcaggg ggttgatacc aaaattttgc taggccattc gaatcaaaaa atgaccgata
     1441 tttacaacga cgcacgcggt aaggaatgga aaaaactggt catttgatga ccagttttgc
     1501 agaggggttt tgcagaggtt ttgcagagaa aattaaaaac gatatcctgc ggagaacata
     1561 aacacccacg gatccagtcg taccgagtct tttacggtag taacaccaga tttgtactta
     1621 gccgtggtat cgatatccat gtaccagact gacatgttaa ccaaccagtc acggttaatc
     1681 agataatcaa cccccacctg cccggcagct ccccaggaat ctttcagact gagatcggaa
     1741 agccctgcct ctttgccatg atcgttaaat ccattatcaa agaaggtggt gtagttaata
     1801 cctgccccaa cgtaaggacg gaatttgctg ctggcatcac caaaatacca ctgcgccatc
     1861 agtgttggcg gcagatggtg aacggttgca atatcgccgg tcgcccgggt gccgatttta
     1921 tggcggaacg gcgtcgctgc cagtaattcc acaccaatgt tgtcggtcgc catataagta
     1981 aacgtcaagc ccagttgagt gttattggtc acgctgaatc cacccagact tcctaacgta
     2041 ccaccagcac cttctgttgg acgtacggtt gcagaacctg cacgcataaa aaattcgcct
     2101 gcttcatgcg caaaggcact gccagagaga agagttgtta ctgccaaagc cgccactgtt
     2161 aactttttca tatccgctcc gtcgttatgg ttataaaaac gtgacgaata tacctatatt
     2221 ggagtaacaa atgatcctac atagatcaca ttacatatga taattcaccc tttattgatc
     2281 tggattaatt tagaagttgc attgagaatc ctggttaacg tgatttaaat caatttttaa
     2341 atattttcgc tacgcaaata ttaactcgat cgccacttgc cgcggtttgc ccacgcctga
     2401 aggccagcaa ttacctccgc tttacaaaga tatgcttttc catacaagac ctgatgctgc
     2461 cacccgaggc ttaaccaggg tacaattgcc cgctaattaa cacctgcata aaactcaagg
     2521 agagtgcatg tctatcacgg cgcagtccgt ataccgtgac accggaaatt tcttccgtaa
     2581 tcaatttatg accattctgt tggtatcgtt gctatgtgcg tttatcacag tggtgttagg
     2641 gcatgttttc tcacccagtg atgcacagct tgcgcagctc aatgacagcg tgcccgttag
     2701 cggcagtagt gggttgttcg acctggttca gaatatgtca ccggaacagc aacaaatttt
     2761 gctgcaggct tcagcggcgt ccactttttc aggattaatc ggtaacgcca ttctcgccgg
     2821 aggcgtaata ttaattatcc agctggtgtc tgcgggtcag agaatcagtg cgctgcgagc
     2881 tattggtgcc agtgcaccga tattgccaaa gttatttatt ctgatttttc tgactaccct
     2941 tttagtacag attggcatca tgctagtggt cgttccggga attatcatgg ccattttact
     3001 ggccctggca ccggtgatgc tggtacagga caaaatgggc atttttgcct cgatgcgtag
     3061 cagtatgcgg ctgacttggg cgaatatgcg tctggtggca cccgcagtac tgagctggtt
     3121 gctggcaaaa acactgttgc tgctttttgc ctcttctttt gccgcattaa ccccggaaat
     3181 tggtgccgta ctggcgaaca ccttgagcaa cctgatttca gccgtattgc tcatctatct
     3241 gttccgtctg tatatgttga ttcgccaata accttaagca tttgattacg gaatcgtaaa
     3301 atgaagcagt ttcttgattt tttaccgctg gttgtctttt tcgcgtttta caagatttat
     3361 gacatctatg cggctactgc ggcgctgatc gtcgccacgg cgattgtgct tatctatagc
     3421 tgggttcgct ttcgtaaggt tgagaagatg gccctgatca cttttgttct ggtggtcgtc
     3481 ttcggtggct tgacgctgtt cttccacaat gatgagttta ttaaatggaa ggttacagtc
     3541 atttatgctc tgtttgcggg agccctgtta gtcagccaat gggtgatgaa aaagccgcta
     3601 attcagcgga tgctgggtaa agaactcacg ctgccgcaac cggtatggtc gaagctgaat
     3661 ctggcctggg ctgttttctt tatcctttgc ggtctggcaa acatctacat cgcattctgg
     3721 ctgccgcaaa atatttgggt caactttaaa gtctttggac tgaccgccct taccttaatc
     3781 ttcacattgt taagcggtat ctatatctac cgccacatgc cgcaggaaga taaatcctaa
     3841 ccagactgcc agtccttcgg ggctggcgct gtatctcctc tgttctcctc aggaaaatca
     3901 tagtagcatc gcgcctgtga ttttcctttt aagtcggttt taccatgtct acaacacata
     3961 acgtccctca gggcgatctt gttttacgta ctttagccat gcccgccgat accaatgcca
     4021 atggtgacat ctttggtggt tggttaatgt cacaaatgga tattggcggc gctattctgg
     4081 cgaaagaaat tgcccacggt cgcgtagtga ccgtgcgggt tgaaggaatg actttcttac
     4141 ggccggttgc ggtcggcgat gtggtgtgct gctatgcacg ctgtgtccag aaagggacga
     4201 catcggtcag cattaatatt gaagtgtggg tgaaaaaagt agcgtctgaa ccaatcgggc
     4261 aacgctataa agcgacagaa gcattattta agtatgtcgc ggttgatcct gaaggaaaac
     4321 ctcgcgcctt acctgttgag taatagtcaa aagcctccgg tcggaggctt ttgactttct
     4381 gcttactgaa tttcggtggt gccgttaatt ttaaacagga tattcaccac aatcccactg
     4441 cctggcttac ccggctcata acgccatctg cgcatcgcat ttttcacctc acgctcaaac
     4501 atgttcgcag gcttggctga gaggatttgt acgttatcca cgcgaccatc cggcgtgacg
     4561 tcaaatttaa ctttaacctg cccttcaatg cgcaatgcct gtgctcgtgc cggatactgc
     4621 ggctgattac ggcttaatgc gcgtggtcct gaagccacac tggtaaccgg cttgctggtt
     4681 gcagccgttg ctgtacttga tgtcaggcgt gccagtgccg tattttcaaa cggtgatgcc
     4741 ggacgcgact ctacgggttt gacatcgcgt tttggctgct cctgtacctt tttcaccggc
     4801 ttcggttttg gcttaggttt cggcttcggc ttttcaatga ccaccggtgc ttcttttggc
     4861 ggttcgggga tcggctcagg ttccggttct ggctctacca ccggctccgg tggcggctga
     4921 acggcttgtg gcggttcgag atcagcaggc gcaaccatcg tgacagaaat tggctgcgca
     4981 ggcgcaggta gttcaataac ctgatgtacc gaggtataga gcagacccgc cacaacagca
     5041 ccatgaatgc agaccgaaag taacgtcggc caggggaagc ggcgaggtaa atcaagggtc
     5101 attgaagtca taatcatttc agtagaaaaa ccaagcctcg attttaaatg caaatagcaa
     5161 tcatattcaa taaggcaaga cgatcaggat cagtgaaaat gtcatttttg ccgtaattaa
     5221 ccttacttta tgcatggtct taacaaagac atcatctttc cgttgcagat cttcgccctt
     5281 tcaaataacg tactttacaa ctttcccgaa caaggagttg tgcccgtgtt gtatgttatt
     5341 tacgctcaag acaaagctga tagcctcgaa aaacgccttt ccgttcgtcc ggcacattta
     5401 gcacgtttac agttactgca tgatgaaggt cgtttgttga cagctggtcc aatgccagcg
     5461 gtagacagta acgatccagg ggctgcggga tttactggct caaccgttat tgctgaattt
     5521 gaatcgctgg aagcagctca ggcctgggcc gatgctgacc cgtacgtagc ggcaggagtc
     5581 tatgaacacg tttcagtgaa accatttaag aaagtgttct gaatagagat tcaatattta
     5641 agggcacggt ttttgcaacc gtgttcttgt ttggcctttc cttatagccg gtcagtgcat
     5701 tcaccaccac aagcagataa taactttatc tgtatttctt tatatgatgt gagcatattc
     5761 aggaaaaatg acctttcctg aggtacagtt gctcgctcaa aacctttgat tactgcggat
     5821 aaagtcatct actattgttt aacacattca cgcgctgcat gtatgttcag aaaatgtgca
     5881 cgctaagata attaaacaaa attgtggtta cagaggtatc agtgagtcac tgggctacat
     5941 tcaaacaaac agcaacaaat ttatgggtga ccctacggca cgacattctc gcgctggccg
     6001 tctttttaaa tggattgctt atttttaaaa caatctatgg tatgtcggtc aatttgctag
     6061 atattttcca tatcaaagca ttttcagagt tggatctttc cttgctggca aacgccccac
     6121 tatttatgct cggcgtcttt cttgtcctga actccattgg cttactgttc cgggcaaagc
     6181 tcgcatgggc aatcagtatc attttgttgt tgatagcgct aatttatacc ctgcattttt
     6241 atccctggtt gaaatttagt attggatttt gcatttttac gctggtgttt ttgctgatac
     6301 tgcgcaaaga cttctcccac agtagcgccg cagccgggac aatttttgca tttattagtt
     6361 tcacgacatt actgttttac tcaacctacg gtgcgcttta tttaagcgaa ggttttaatc
     6421 cgcgaataga aagtttgatg accgcgttct atttttcgat agaaaccatg tcaaccgtcg
     6481 gctatggcga tattgtccct gtttctgaat cagcacgatt gttcactatt tcggtcatta
     6541 tttccggtat taccgttttt gccacatcta tgacctcaat ttttggtccg cttatccgcg
     6601 ggggattcaa caaacttgta aaaggaaaca atcatacaat gcatcgtaaa gatcatttta
     6661 ttgtttgcgg acattcgatt ctcgccatca atacgattct gcaactgaat caacgcggac
     6721 aaaacgtaac ggttatcagc aacttgcctg aagatgatat caagcaactt gagcaacgct
     6781 taggcgataa cgctgatgtt atccccggtg acagtaatga cagttcagta ttaaagaaag
     6841 cgggaatcga tcgttgcctg gccattctgg cgctgagtga taacgatgca gataacgcgt
     6901 ttgttgtact ctcggcaaaa gatatgagca gtgatgtcaa aacagttctc gccgtcagtg
     6961 atagcaaaaa cctgaataag attaagatgg tacatccgga tatcattctc tcaccgcaac
     7021 tgtttggcag cgaaattctg gcgcgagttt taaatggtga agagattaat aatgatatgc
     7081 tcgtttcaat gttgttgaac tccggtcatg gcattttcag cgataacgat gaacaagaaa
     7141 cgaaagctga cagtaaagaa tcggcgcaaa aatagagttt aaagatggag gagtaaactt
     7201 attgggcacc gcaatggtgc ccagtacatt tagatattgt agatcgcaaa caataacgag
     7261 ttacgctgtt tgtttagaat gcacttcctg atgctgtgga tacgcatatt tcggcgcgat
     7321 tgcccctcaa gccaacgcga tttacgtcgg ctagcctgac gctgcatgcg ccagcgcccc
     7381 acttccgttc tactccgctt catgttaact actctatgca ataacagaat ggtcattata
     7441 cccccgcccc cgacgctgac cagtgctttt cccgcgtttt tatttgccag atgaatccat
     7501 atgcgtaaac tcataacaat gcgctttcaa aaggatttct aatctatgac aaccgtttat
     7561 acgttggtga gttggttggc cattctggga tactggttgc tcattgcagg cgtaacttta
     7621 cgcattctaa tgaaacgacg cgcagttccc tccgcgatgg cctggctgtt gattatttac
     7681 attctgccgt tagtcggaat tattgcctat cttgccgttg gcgagctcca tttaggcaaa
     7741 cgccgcgctg agcgcgccag agcgatgtgg ccttccaccg caaaatggct taacgacctt
     7801 aaagcctgta agcatatctt cgccgaagaa aatagcagtg tcgctgcgcc attattcaag
     7861 ctttgcgagc gtcgtcaggg gatcgctggg gtcaaaggga atcagctaca actgatgacc
     7921 gagtcagatg atgtgatgca ggcgttaatc cgcgacatcc agctcgcgcg ccataatatt
     7981 gagattgtgt tttatatctg gcagcccggc ggcatggcgg accaggtggc tgaatcatta
     8041 atggccgctg cgcgacgcgg cattcattgc cgattgatgc tcgactccgc cgggagtgtg
     8101 gcttttttcc gcagcccgtg gcccgagcta atgcgtaatg ccggtattga agtggtcgaa
     8161 gccttaaagg tcaatctgat gcgtgtgttt ttacgccgta tggacctgcg ccaacatcgc
     8221 aagatgatca tgatcgataa ttacatcgcg tacaccggca gcatgaatat ggtcgatcct
     8281 cgctacttca aacaagatgc gggcgtaggg caatggattg atctgatggc gcgtatggaa
     8341 ggccccatcg ccaccgcgat ggggattatt tattcctgcg actgggagat tgaaaccgga
     8401 aaacgtattc tgccgccacc accagatgtc aatattatgc cgtttgaaca ggccagcggt
     8461 cacaccattc acacaattgc ttctggcccc ggctttccgg aagatctcat tcaccaggca
     8521 ttattgactg cggcttattc ggcgcgtgaa tatttgatca tgaccacgcc ctactttgtg
     8581 ccaagcgatg atttacttca tgcgatttgc acggcggcac agcgcggggt ggatgtcagt
     8641 attatccttc cgcgaaaaaa tgactcgatg ctggtcggct gggccagtcg cgcattcttt
     8701 acggaacttc ttgctgctgg ggttaaaatt tatcagtttg aaggcgggtt actgcatacc
     8761 aagagcgtgc tggtcgatgg cgaactaagt ctggttggca ccgttaacct tgatatgcgt
     8821 agtctgtggc tgaatttcga gattaccctg gcaatcgacg ataaaggttt tggtgcagac
     8881 cttgccgccg ttcaggacga ttatatttcg cgttcacgtc tgctcgatgc ccgtttatgg
     8941 ctaaaacgtc cattatggca acgtgtcgcc gagcgactgt tttacttctt cagcccgttg
     9001 ctgtaaaacg tctccaacag aggttaaaca ggtagtgact atggatatgg atctaaacaa
     9061 tcgcctgact gaagatgaaa cgcttgagca ggcctacgat atttttctcg aactggctgc
     9121 cgacaatctc gacccagctg atgttctgct gttcaatctt cagtttgaag agcgcggcgg
     9181 cgcggagtta ttcgatccgg tggaagactg gcaggaacat gttgatttcg atttgaaccc
     9241 ggactttttt gccgaagtgg tgattggtct ggcggacagt gaggatggtg aaatcaacga
     9301 tgttttcgcc cgcattctgt tatgccgcga aaaagatcac aaactttgcc atatcatctg
     9361 gcgggaataa ataatcgctt tcggcgtgaa aaagggctga caactgtcag cccttattgt
     9421 gtttaaagcg gatcgacttt caggcaagaa acggcgtgtc tgaaactccc ttccagcaca
     9481 ggacgtgttt tggcgcactc cggaccggca atcgggcaac gggtacggaa aacacaaccg
     9541 gaaggcgggt tgatcggcga cggtaattcc ccttccagta actggatggt tttgttcttc
     9601 tccagatccg gatcaggtat ggggactgcc gacatcaatg ccctggtgta aggatgtagt
     9661 ggattgtggt agacctcatc ataggtcccc agttctaccg catggccgag atacatcacc
     9721 aacacacgat cggaaatgtg ttttaccacg gccaggtcat gagcaataaa aattaatgac
     9781 aatcccatct cacgttgcag ctgctggagc aggttgacca cctgcgcctg aattgacacg
     9841 tccagcgccg acaccggctc atcacagata atcagcttcg gttcaagaat aagagcacga
     9901 gcaatcccga tacgctggca ctgcccacca gagaactcat gcggatagcg gttaatcagg
     9961 ttaggcaata acccgacttt cagcatcatc gccttcacgc gctcgcgaac ttcctgacgt
    10021 gacattttcg gatgataagt acgcagtggt tcagcgatga tctcgccgat ggtcatgcgc
    10081 gggtttagcg atgccaacgg atcctggaaa atcatctgaa tatcactgcg aacggcacgc
    10141 cattcatcgg gcttaatgcc cagcaactct ttacctaacc aggcaacatg accgtcggtc
    10201 gctttaacca ggccaatgat ggcgcgagca aaggtggact taccgcatcc cgattcccct
    10261 accacaccta atgtttcccc ttcatacagg cgaagcgtga caccatcgac ggctttgagc
    10321 gttttcggcg gttgccagaa ccactgtttg ccatctttga tttcaaagtg cactttcaga
    10381 tcggcgattt caaggaggac ttttcttcct tcggttacag cattcataac agttcctcca
    10441 ccggtttaaa gcaagcacac agacggccaa gcgtaaactc ttccagcggc ggtgcgctac
    10501 tacaaatttc catcgcatgc ggacaacgtg gctggaacgg gcaacctttc ggtaatcgca
    10561 gcaggtttgg cggattaccc gggatggtca acattgtttc accttccgca tcgagacgcg
    10621 gcaccgcgtt gagcaaaccg atagaataag gatgaacggg ttgataaaag acatcgcgcg
    10681 cgttgccata ttccatcgtg cgcccggcgt acattaccag cactttgtca cagatccccg
    10741 ccaccacgcc aagatcgtgg gtaatcataa tgatggcggt attaaattcc cgtttcagtt
    10801 catttaatag cgtcatgatc tgcgcctgta cggtgacgtc cagcgctgta gtgggttcat
    10861 ccgcaatcag cagcttaggt cgacatagca atgccatcgc aatcataact cgctgacgca
    10921 tgccgccaga aaactcgtgc gggtacattt tcatgcgttt acgtgcttcc ggcattttta
    10981 ccgcatcgag catccgcacc gactcttcaa acgcctcagc tttgctcatg ttcttatgca
    11041 gcatcagcac ttccatcaac tgctcaccga cgcgcatata gggattcaac gaagtcattg
    11101 ggtcctgaaa aatcattgag atttgttcag cgcgcagttt attgagttca tgctctggca
    11161 aattgaggat ttcacgccca ttgaaggttg ccgatccgcc aatacgtccg ttggcagcca
    11221 acaggcccat caacgcaaat gcagtttgcg atttacccga accagactca cctacaatac
    11281 ccagcgtttc tccggcacgt agggaaaaat tcaaatcatt gaccgtcgtg acgtcgccgt
    11341 ccggggtact aaaggtgaca cgcaaatctt tcacgttcag cagtgcgtca gcctgttgtt
    11401 gtgcgagcgg cacagttgca gtttcaatta cgctcatggc tgcactcctt aacgatcttt
    11461 cgggtcgagg gcatcacgca agccatcgcc gataaagttg aaacaaaaca gcgtcaccac
    11521 gaggaatccc gctgggaaca acaataacca tggagagact tccatcgagt tcgcgccatc
    11581 actcagcaat gcaccccagc tgcttaacgg ctcttgcgta cccaacccca ggaagctaag
    11641 gaaagattca aagaggatca tgctgggcac cagaagtgat gcgtagacca ccaccacacc
    11701 gagtacattc ggcacaatgt ggcgaataac aatgcccgat gtcgatacac cgccaacttg
    11761 tgccgcctca ataaactctt tgcgcttcag actcagggtt tgcccacgca caatacgagc
    11821 catatccagc caggaaacca tgcctatcgc cacgaaaatc agcaggatgt tttgaccgaa
    11881 aaaggtcacc agcaaaatga cgaagaacat gaatgggaag gagttgagga tttccagcag
    11941 acgcatcatt accgaatcca ctttaccgcc cagataaccg gaaagcgaac cataaagtgt
    12001 cccgacgacc acagccacca gtgccgcagc aacaccgacc atgagtgaga tacgcccacc
    12061 aatcgcaacg cgcacaagca ggtcgcgacc ggatgagtca gtaccaaagt agtgaccgga
    12121 ctccatatcc ggggcgctgg acatcatcgc ccagtcagta tcgtcatagg caaactggga
    12181 aagcatcggt gccaggatta caaataacgc gatcagcacc agcaccatca gactggcaac
    12241 cgccgcacgg ttatgaataa aacgtcgacg tgcgtcctgc cacaagctgc gcccttcgac
    12301 ctccagcttt tcactgaaat tttccagcgt ctcgctgttt ttcttactta acatcatcgc
    12361 gagctccagt atcagtaacg gattttcggg tcgataaccg catatagcac atcgacaatg
    12421 gcattaaaca aaatggttaa agcaccaacc aggatggtca ggcttaacac taaggaatag
    12481 tcacggttca atgcgccatt aacgaacagt tgtccaatcc ccggcaaacc ataaatggtt
    12541 tcgataacca tagaaccggt aataatgccg acaaatgcgg ggcccatata agagagcacg
    12601 ggtaacagag caggttttaa tgcgtgacgt aaaatgatcc gccgcatagg taaccctttc
    12661 gcccgggcag tacgaataaa attggagtgt aatacttcaa tcatagagcc acgggtaata
    12721 cgcgcaatac tggcgatata agcgagtgac aacgccacca tcggcaatat catgaattta
    12781 agcgccccac cattccagcc accgcccggc agccaatgca aaatgatcgc aaatatcatg
    12841 actaataatg gtgcaaccac aaaactgggg ataacaaccc cggtcattgc cagccccatc
    12901 acggtatagt cccatttggt gttttgtttt aatgcggcaa taacgccagc actaacaccc
    12961 agtattaccg caaggaaaaa tgctgcggct cccagtttgg cagaaacggg aaaactggat
    13021 gcaaccaagt cattgaccga ataatcttta tatttaaacg atggaccgaa atcaccgtgc
    13081 gccagttgtt tcaggtagct gaaatactgt gtcatgatcg gatcattaag atgatatttc
    13141 gcttcgatat tggccatcac ttctggcggt aaagtacgtt cgccggtaaa agggcttccc
    13201 ggcgcgaggc gcatcataaa gaacgaaata gtaataagaa taaatagcgt cggaatcgct
    13261 tccagacagc gacgtagaat aaattttaac attgccctac cttctggcct gtgccttctg
    13321 taatgcgata aaaatcagac accgtggagc aggacactcc tgccccacgt attgccatta
    13381 gtgcttcaca atgtacatat tccgggtata ggtattatcc agcggatctt tgccggtata
    13441 gccaccaacc cacggtttca ccagacgcgc attcacgtag taataaacag gaacaatggc
    13501 cgaatcctta tccagctgtt gttctgcttt agtgtacaga gctgtgcgct gcgcctcgtc
    13561 agtcactttc agcgtttccg ccataatgct gtcaaaggcc gggctcttat aatgcgcggt
    13621 attcatcgag ctgttcgaaa gcatggtgtt caggaaggaa gttggttcgt tgtagtcagc
    13681 acaccagcct gcacgggcca catcaaaagt accctggtga cgggtgtcga ggaacgtttt
    13741 ccactcctgg ttaaccagtt tgacgtttac accaatgttt ttcttccaca atgaagaggc
    13801 agcaatcgcc agctttttat gcagatcgga ggtgttatac aacaggttga ttgtcaacgg
    13861 tttgtctgcg gtataacccg cttcagccag cagttttttc gcttcttcgt tacgtttttc
    13921 ctggctccag ccaaaccatt ccggctgagt caattttgcg ccatcagtat acggtggagt
    13981 gtaaccatag gcgggcatgt cgccctgcgc tttcacttta ttaacaatga tatcgcggtc
    14041 catacctagt ttcagtgcgg tacgcacacg cacatcgttg aatggcggtt tctggttgtt
    14101 aatttcgtaa tagtaagtgc acaggtatgg atcaacgtga acttcgtccg ggatctcttt
    14161 tttcagcttc tggaacaatt cgatcggcat gttgttataa gtcatgtcga tttcaccact
    14221 acggtagcgg ttgacatcgg taacttcaga agcaataggc aaataggtta cctggttaat
    14281 aacggttttc gcgttgttcc agtaggtcgg gctgcgttca agaacgattc gttcgtttac
    14341 gacccaatct tttaaggtat aggcaccgtt ggtgacgata ttaccaggct gggtccattt
    14401 ttcgccgaat ttctcgatag cggcttttgg caccggtgaa gttgatgggt gaacaagtaa
    14461 tttatagaag tacggaacgg gttcacttaa ggtgacttct aatgtgtgat catcaatagc
    14521 tttcacaccg agatcggtaa tcggtttttt cccttcaaga atttcatcaa taccggcgat
    14581 atgcccatat tgcagataac tggcatacgg agaagcagtg ttcggatcaa cagaacgttg
    14641 ccagctatac acaaagtctt gtgctgtgac tggcgtacca tcagaccatt tcgcatcttt
    14701 acgcaaatgg aaggtccaga ctttcgcgtc tttattatcc caggattcag cgacgccagg
    14761 tgccggatga ccgtcaagat cgctgaccag taagccttca aacaggtctc ggctgatatt
    14821 agactccgga acaccttcaa ttttgtgcgg atctaatgac tgaacttctg aaccattgtt
    14881 acgtaccagc gtttgttttt ccgccagtgt gacgcctgcg ggtacatcag ctgccagcgc
    14941 gacattccct gccattagcg cagccagaac gccagctgct actaaacttc tcttggtgat
    15001 gttggtcatt gttttttgga ctccctcatt ataattactg gtattaacca gcatgtgtaa
    15061 tcccggattg ggaccctgca caggctcgga gactttctgc tgtcaggtta ctttatcgtt
    15121 acggattgct atcaccgact ttatttattc tggtgattca gacgatcacc ttatgtcgat
    15181 tcgtactgtg tctcccctgt cgagacacat tattctttcg cgacgctctt taattgttaa
    15241 ttgttaatta ttctcattac caataaagaa tcgtctggcg gttgcccgga aagtactaaa
    15301 tgcggttctt actcgccaat acattttgca aaattgttaa gcaattctct tttattgacc
    15361 gtatcagaat ctatagcttg catccatgcc ttaacaaaca tctttcaatt caatcaaata
    15421 gagattggct aagcatgtct atcttttctg attacagttt cagtattgaa atgcacaata
    15481 atttaacaat tgattattat ttagcacttt cctctaccaa aggcagtgga attactaata
    15541 tcatttcaat tattcttcag caagcccagg attatgatgt ggcgaaaata acatgatggt
    15601 gaggagaagc agttaagaaa atccttataa ctaactgtaa tttgagaaga atatttttac
    15661 ctattccttt tgcttttgat aactgcgcg
//
LOCUS       DAJTMC010000092        15633 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.092, whole genome shotgun sequence.
ACCESSION   DAJTMC010000092 DAJTMC010000000
VERSION     DAJTMC010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15633
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.092"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            336..428
                     /locus_tag="OO130_002581"
                     /pseudo
     CDS             336..428
                     /locus_tag="OO130_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004120448.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            432..611
                     /locus_tag="OO130_002582"
                     /pseudo
     CDS             432..611
                     /locus_tag="OO130_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            945..3224
                     /gene="ydeP"
                     /locus_tag="OO130_002583"
     CDS             945..3224
                     /gene="ydeP"
                     /locus_tag="OO130_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HCR5365939.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRQDVIAMFDMNKP
                     EGFDCPGCAWPDPKHTASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIEHISGLSQTQIAELADAYAAADRTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFTPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            3472..3669
                     /gene="safA"
                     /locus_tag="OO130_002584"
     CDS             3472..3669
                     /gene="safA"
                     /locus_tag="OO130_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HCR5365940.1"
                     /translation="MHATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLG
                     ISEMGRIARDLTHFIINSLQD"
     gene            3744..4505
                     /gene="ydeO"
                     /locus_tag="OO130_002585"
     CDS             3744..4505
                     /gene="ydeO"
                     /locus_tag="OO130_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HCR5365941.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFTENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQSHTGMNTGNTMSALAI"
     gene            4907..6589
                     /locus_tag="OO130_002586"
     CDS             4907..6589
                     /locus_tag="OO130_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HCR5365942.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQDKKQGEPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYNLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            6641..7798
                     /locus_tag="OO130_002587"
     CDS             6641..7798
                     /locus_tag="OO130_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416014.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HCR5365943.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIAASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CSFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(7764..7886)
                     /locus_tag="OO130_002588"
     CDS             complement(7764..7886)
                     /locus_tag="OO130_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5365944.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
     gene            8095..9780
                     /locus_tag="OO130_002589"
     CDS             8095..9780
                     /locus_tag="OO130_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HCR5365945.1"
                     /translation="MITIPITFCMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGTLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQAELIALSNAESLQRQELRDNFHTIKENWHRLMNRQR
                     WLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDE
                     LAELAAVIDRLYEFHQLTEQRPTNKPKNCQHAVQVANASIRTPDNKIILENLNFHVSP
                     GKWLLLKGYSGAGKTTLLKTLSHCWPWFKGDIFSPADSWYVSQTPLIKTGLLKEIICK
                     ALPLPVDDKSLSEVLHQVGLGKLAARIHDHDRWGDILSSGEKQRIALARLILRRPKWI
                     FLDETTSHLEEQEAIRLLRLVREKLPTSGVIMVTHQPGVWNLADDICDISAVL"
     gene            9818..12190
                     /locus_tag="OO130_002590"
     CDS             9818..12190
                     /locus_tag="OO130_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HCR5365946.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRSDWLTSHIDENNKSAFNQGSSGSTYFSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVVGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYSGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQNLKTPYNDELAMGLQQKIGKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHIRQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            12247..15030
                     /locus_tag="OO130_002591"
     CDS             12247..15030
                     /locus_tag="OO130_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCR5365947.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNGLRYMI
                     YPHAQPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKKALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFIEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNELAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGARVILAKSAGEEQKLQITAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIVQFTA
                     ADALAADRQLFSSPADITFVIVGNVSEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLAKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNTYTGVLLPK"
     gene            15392..>15633
                     /locus_tag="OO130_002592"
     CDS             15392..>15633
                     /locus_tag="OO130_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR5365948.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSI"
BASE COUNT         4552 a         3398 c         3426 g         4257 t
ORIGIN      
        1 tggtgacggc agttgaaacc aatgtgttga ggttgcatgg acggttaagc cgagaatacg
       61 gtagtaagtg agatatgggg tttggtcgtt gttaatgagc aaccatgacc atgaccgcca
      121 acgactggat tctcccttgg ttatgttggt gcgcataatg tatattatgt taaatcatat
      181 ctgagtgatg cgaagtgctt ttctggtagt cgttattcgt tcagggttcc agaacttaag
      241 cctctcctct ttcataattg gcctgtgcat gacggatata ggctggctgg tatgcagatt
      301 tgccgtttgc cgcatcttcc cagcgttgaa gactcaagct ggcaaaccgc gtgcaaatga
      361 gcacgccctc tttccatcaa cacttcaggc agcttgcggg aatgagtcca ctacggtatc
      421 agaaatggga agtgggtgtc tctctgatgc gtaatggaaa aatactggct acgggagaaa
      481 atgtctccct ggggacagta aataaaagca aagtatctct gggtttaagc gcaacctatg
      541 gacaaacagg aaacaaggtt gcagccggaa ccgtccagtc agtcattggt gtcactttta
      601 tctatgaata attgtgattt tgatcttagt ctatgcgctc cattcacgaa aattgcgcgg
      661 ataattattt tgtgaaggct attatcctac acctgtaaga aaatccgcgc acaattgaag
      721 ccagagtctt attttttata ttgaaaaata tgttaattta aacactatga gagatgatgc
      781 ttaacttatc gtatttaatc tattgtttaa cgataataga ttaaaaatag aaagagagta
      841 gtcccctact cttatatatc catgttggcg ataatccctt tgtatgtact gtgcatcatc
      901 gctattacaa atcctaataa ttcatttcca cacaggataa gtagatgaag aaaaaaattg
      961 aatcctacca gggtgctgca ggtggttggg gtgctgttaa atccgtagcg aatgcagtac
     1021 gtaagcagat ggatatacgc caggatgtta ttgccatgtt tgacatgaat aagccagagg
     1081 gctttgactg tccgggttgt gcatggccag atcctaagca cactgcgtca ttcgacattt
     1141 gtgaaaacgg cgcaaaagca atcgcctggg aagtcacgga taagcaggta aatgcctctt
     1201 tctttgctga gaatacggtt caatcattac ttacctgggg agatcacgag cttgaggctg
     1261 cgggacgtct cactcagcct ttgaaatatg atgccgtcag cgactgttac aagccattaa
     1321 gctggcaaca agctttcgac gaaattggcg cacgccttca aagctatagt gatcccaatc
     1381 aggttgaatt ctatacttcg ggccgcactt ccaatgaagc tgcctttctt tatcagcttt
     1441 ttgcccgtga atacgggagc aataactttc ccgactgctc caacatgtgc catgaaccga
     1501 ccagtgtggg tttggcagcg agtatcggcg tgggtaaagg gactgtgttg ctggaagact
     1561 ttgagaagtg cgatttagtc atttgcattg ggcataaccc tggtacaaac cacccccgca
     1621 tgctgacttc gttgcgcgct ttagtgaaac ggggagcgaa aatgatcgcc atcaatcctt
     1681 tacaggaacg tggcctggag cgatttaccg caccgcaaaa cccgtttgaa atgctgacga
     1741 actctgagac tcagttggcc agtgcgtact acaacgtgcg cattggtggc gatatggcgt
     1801 tgctcaaggg gatgatgcgc ctgttaattg agcgcgatga tgctgcaagt gccgcaggtc
     1861 ggccctcatt gcttgatgac gaatttattc aaacgcatac cgtcggcttt gacgagctac
     1921 gccgtgacgt tctcaattcc gagtggaaag atatcgaaca tatttctgga ctaagtcaga
     1981 cacaaatcgc cgaactggct gacgcatatg ccgctgccga tcgcaccatt atctgttacg
     2041 gaatggggat cactcagcac gaacatggta cccagaatgt acagcaactg gtcaatctgc
     2101 tgttgatgaa aggtaacatt ggcaagcctg gtgcgggtat ctgcccacta cgaggtcact
     2161 ctaatgtaca gggcgaccga accgtcggta tcaccgagaa accgtctgca gagtttctgg
     2221 ctcgcctggg tgagcgctat ggcttcaccc cacctcatgc acctggacat gctgcaattg
     2281 ccagcatgca agcaatatgc acggggcagg ctcgagcatt gatctgcatg gggggcaact
     2341 ttgcactggc aatgccagat cgggaagcga gcgctgtacc gttaacgcaa ttagatttgg
     2401 cggtacacgt agccactaag cttaaccgct ctcatctgct gaccgcacgg catagctata
     2461 ttctgccggt tctggggcgt agcgagattg acatgcaaaa aagcggtgcg caggcggtaa
     2521 ccgttgagga ttcaatgtcg atgattcatg cctcgcgtgg cgtgttaaaa cccgccggtg
     2581 taatgctgaa atcagagtgt gcagtggtcg cgggaatcgc gcaggcagca ctaccccaga
     2641 gcgtggtagc ctgggagtat ctggtggaag attatgatcg cattcgcaat gacattgaag
     2701 ctgtgttacc agagttcgcc gactataacc agcgcatccg acatcctgga ggttttcacc
     2761 tgataaatgc agctgctgaa aggcgctgga tgacgccgtc aggtaaggct aatttcatta
     2821 ccagcaaagg gctgttagaa gatccctctt cagcgtttaa cagtaagctg gtcatggcga
     2881 cagtacgcag ccacgatcag tacaacacga cgatttatgg tatggatgat cgctatcgag
     2941 gggtattcgg tcaacgagat gtggtcttta tgagtgctaa acaagctaaa atttgccgtg
     3001 taaaaaacgg cgaaagagtt aatcttattg cgcttacgcc agacggtaag cgcagctcac
     3061 gccgcatgga tagattaaaa gtggtcattt accctatggc tgaccgctca ctggtgacct
     3121 attttccaga atcgaatcac atgctaactc ttgataacca cgatccatta agtggcattc
     3181 ctggttataa aagtattccg gttgaattag aaccatcaaa ttaattcgcc tcctcatttc
     3241 ttctgctgtc atccgcacag cagaagaatt tctcattgac tattatcgcg caatttgctc
     3301 acattgatca aattaaacca cacactataa gatataaacc tctgcctaca gctgtaagaa
     3361 actccgctca gtactgaagt gccagtccta tttcctcttt tctccagcct gttatattaa
     3421 gcatactgat taacaatttt taacgttatc cgctaaataa acatatttga aatgcatgcg
     3481 accacagtga aaaacaaaat cacgcaaaga gacaactata aagaaatcat gtctgtaatt
     3541 gtggttgtct tattactgac ccttacgttg atagccattt tttcggcaat tgatcagctg
     3601 ggtatttcag aaatgggtcg cattgcaaga gatcttacac atttcattat caatagtttg
     3661 caagactgaa aataaacagc aaattataaa tatgaaatgt taaaaaagta tcgataaaaa
     3721 ctttattgtt ttaaggagat aaaatgtcgc tcgtttgttc tgttatattt attcatcatg
     3781 ccttcaacgc taacatttta gataaagatt acgccttctc tgacggcgag atcctgatgg
     3841 tagataacgc tgttcgtacg cattttgaac cttatgagcg gcattttaaa gagatcggat
     3901 ttactgaaaa taccattaaa aaatatctac aatgcactaa catccagaca gtgacggtgc
     3961 ctgttcctgc gaagttttta cgtgcttcaa atgtaccgac tggattgctt aatgaaatga
     4021 ttgcttatct caactcggaa gaacgcaatc atcataattt ttcagaactt ttgctttttt
     4081 cttgcctgtc tatttttgcc gcatgcaaag gtttcattac actattaact aacggtgtgc
     4141 tatccgtttc cggaaaagtg agaaatattg tcaacatgaa gctggcgcac ccatggaagc
     4201 tgaaagatat ttgtgactgc ctgtacatca gtgaaagcct gttgaagaaa aaactaaagc
     4261 aagagcaaac gacattctca cagattctat tagatgccag aatgcagcac gcaaaaaatt
     4321 tgatacgcgt agaaggttca gtcaataaaa ttgccgaaca atgtggttat gccagtactt
     4381 cttattttat ttatgcgttc cgcaaacatt tcggcaacag tccgaagaga gtttctaagg
     4441 agtaccgttg tcaaagtcac acgggtatga atacgggcaa cacgatgagt gctttagcta
     4501 tttgattatt tgctaacgag tagtcaacca cgcacgctac gtaagaatta tatggggcag
     4561 ccattccctg ccccgcgttg tttttaggcg atatatttat tgaaataaat aagtgacatc
     4621 catcacatat ttatgcactt gcataacctg ttgcatgatt atttatgttc tcaattcagc
     4681 atcttgtcag taaaatgcaa taatttatta aatatcaata aattagttgt ttatcggcga
     4741 gaaattactt aatagaacag aaagtaatgt caacgcttta tggactgtta tttccctttt
     4801 tttagctaaa tctgctatct ctttatgtga ctaacttcac ttacatccac ttatttctct
     4861 tcgtaaaatt acttcggaat taagtacaat aagaagagga acatttatga agtctgcatt
     4921 aaagaaaagt gtcgtaagta cctcgatatc tttgatactg gcatctggta tggctgcatt
     4981 tgctgctcat gcggcagatg atgtaaagct gaaagcaacc aaaacaaacg ttgctttctc
     5041 agactttacg ccgactgaat acagtaccaa aggaaagcca aatattatcg tactaaccat
     5101 ggatgatctt ggttatggac aacttccttt tgataaggga tcttttgacc caaaaacaat
     5161 ggaaaatcgt gaagttgtcg atacctacaa aatagggata gataaagcca ttgaagctgc
     5221 acaaaaatca acgccgacgc tcctttcatt aatggatgaa ggcgtacgtt ttactaacgg
     5281 ctatgtggca cacggtgttt ccggcccctc ccgtgccgca ataatgaccg gtcgagctcc
     5341 cgcccgcttt ggtgtctatt ccaataccga tgctcaggat ggtattccgc taacagaaac
     5401 tttcttgcct gaattattcc agaatcatgg ttattacact gcagcagtag gtaaatggca
     5461 cttgtcaaaa atcagtaatg tgccggtacc ggaagataaa caaacgcgtg actatcatga
     5521 caacttcacc acattttctg cggaagaatg gcaacctcaa aaccgtggct ttgattactt
     5581 tatgggattc cacgctgcag gaacggcata ttacaactcc ccttcattgt tcaaaaatcg
     5641 tgaacgtgtc cccgcaaaag gttatatcag cgatcagtta accgatgagg caattggcgt
     5701 tgttgatcgt gccaaaacac ttgaccagcc ttttatgctt tacctggctt ataatgctcc
     5761 gcacctgcca aatgataatc ctgcaccgga tcaatatcag aagcaattta ataccggtag
     5821 tcaaacagca gataactact acgcttccgt ttattctgtt gatcagggtg taaaacgcat
     5881 tctcgaacaa ctgaagaaaa acggacagta tgataatact attattctct ttacctccga
     5941 taatggtgcg gttatcgatg gtcctctgcc gctgaacggg gcgcaaaaag gctataagag
     6001 tcagacctat cctggcggta ctcacacccc aatgtttatg tggtggaaag gaaaacttca
     6061 acccggtaat tatgacaagc tgatttccgc aatggatttc tacccgacag ctcttgatgc
     6121 agccgatatc agcattccaa aagaccttaa gctggatggc gtttccttgc tgccctggtt
     6181 gcaagataag aaacaaggcg agccacataa aaatctgacc tggataacct cttattctca
     6241 ctggtttgac gaggaaaata ttccattctg ggataattac cacaaatttg ttcgccatca
     6301 gtcagacgat tacccgcata accccaacac tgaggactta agccaattct cttatacggt
     6361 gagaaataac gattattcgc ttgtctatac agtagaaaac aatcagttag gtctctacaa
     6421 tctgacggat ctacagcaaa aagataacct tgccgccgcc aatccgcagg tcgttaaaga
     6481 gatgcaaggc gtggtaagag agtttatcga cagcagccag ccaccgctta gcgaggtaaa
     6541 tcaggagaag tttaacaata tcaagaaagc actaagcgaa gcgaaataac taaaccttca
     6601 tgcggcggaa ttttccgccg ccttattgag cgagatagcg atgcacgtta cagccaagcc
     6661 ctccagtttt caatgtaatc tcaaatgtga ttactgtttt taccttgaaa aagagtcgca
     6721 gtttactcat gaaaaatgga tggatgacag cactctgaaa gagttcatca aacaatatat
     6781 cgcagcgtct ggcaatcagg tctattttac ctggcaaggc ggtgaaccca ctctggctgg
     6841 cctggatttt ttccgtaaag ttattcacta tcaacaacgc tatgcaggcc aaaaacgtat
     6901 ttttaatgca ttacaaacga atggcatttt attgaataat gaatggtgtt cctttctcaa
     6961 agaacatgaa tttctggttg gtatctcgat cgatggcccc caggagttac atgaccgtta
     7021 cagacgcagt aattcaggta acggtacttt tgcaaaagtg atagcagcca tcgagcgtct
     7081 gaaatcatat caagttgagt ttaatacgtt aaccgtcatt aataacgtta atgtccatta
     7141 ccctcttgag gtttatcatt ttttaaaatc tatcggcagt aaacatatgc aatttatcga
     7201 attgctcgaa accgggacgc cgaatattga tttcagtggt catagtgaga acacattccg
     7261 tatcattgat ttttctgtgc ctcccacggc ttatggcaag tttatgtcaa ccatttttat
     7321 gcaatgggtt aaaaacgatg tgggtgaaat tttcattcgt cagtttgaaa gctttgtcag
     7381 ccgttttttg gggaatgggc atacaagttg tattttccag gagtcctgca aggataatct
     7441 ggttgttgaa agtaatggag acatttacga atgcgaccat tttgtctatc cacagtacaa
     7501 aattggaaac attaataaat ctgaactcaa aacgatgaac agtgtacaac tgacagcgca
     7561 aaaaaaacgg atttcagcga aatgtcagca atgtgcatat aaacctatct gcaatggcgg
     7621 ttgtcctaag catcgtatta ctaaagtaaa caatgagact gtttcttatt tttgcgaagg
     7681 ttataaaatc cttttttcaa ccatggtacc ttatatgaac gccatggttg agttagctaa
     7741 gaacagagta ccgctttacc acattatgga tgttgcaaaa caaatggaga ataattaata
     7801 acgctctctt ttaactccat tcttagcctc cttcttatga ccttaacaag gcacccgaca
     7861 taaattcctg tcgggtgttt cgtcatcatc atcttccctt gcgaaaatcc tggcttacgt
     7921 ggtatgcgtc agggcattag caataccctt gattatcaat agcataacca taaccataac
     7981 cactgaaaat taaaaatttc tatatatttc atattgttaa atgatactta aataaatttc
     8041 atcagtaaat cattgccata gataatcatt ggatggcaca atcaattttc gctaatgata
     8101 accattccca ttacgttttg tatgttgata gcaaagtatc tctgtctgct gaagcccttt
     8161 tggctgcgta agaataataa aacttcagtg ttgttaatca ttattatcct cgcaatgatc
     8221 cttggcgtgg ttaaaattca ggtctggtta aacgactgga ataatgattt tttcaatgcg
     8281 ctaagccaga aagaaaccga taagctttgg cagcttgttc tttggttccc tgccctgctg
     8341 gggatctttg tattaatatc cgtaaacaaa acatggttaa ttaaactact gactattcgc
     8401 tggcgcgaat ggttaacaga ttactacctt aatcgctggt ttgcagataa aaactactac
     8461 ttcacgcaga tctacggcga gcataaaaat acagataacc ctgaccaacg tatcgctgaa
     8521 gacattctct tactgatcag caaaacgctc agtctttcat ttggcttcat ccagtcactt
     8581 agcatgctga tcacctttac cgttattctt tggcaaagcg cgggtacgct ctcattcact
     8641 gttggtggaa cagaatggaa tatccagggg tatatggtct ataccgtcgt gctcattgtg
     8701 atcggtggaa ctttatttac ccataaggtt ggtaagcgta ttcgtccgct taatgtggaa
     8761 aaacaacgta gcgaagcgac atttcgaact aatcttgtgc agcataataa gcaggcagaa
     8821 ctgattgcgc taagcaatgc tgagtctcta caacgccagg agttgcgcga caattttcat
     8881 accattaaag agaactggca tcgtttaatg aatcgtcagc ggtggcttga ttactggcaa
     8941 aatatttatt cgcgctcgct tagcgttctt ccctactttc tgttattgcc gcagtttatt
     9001 agcgggcaga tcaatctggg cggactgatg aaatcgcgcc aggcatttat gctagtatcg
     9061 aacaatttaa gctggtttat ttataaatat gacgaacttg ctgaactggc tgcggttatc
     9121 gatcgcttgt atgagttcca tcaactcact gaacagcgcc ctacgaataa gcctaaaaat
     9181 tgccaacatg cggtacaagt ggctaatgcg agtattcgta cgcctgataa taagatcata
     9241 ttagagaact tgaactttca tgtttcgcca ggcaaatggc tattactgaa aggctactct
     9301 ggtgcgggaa aaaccacact gcttaaaaca ttatcccact gctggccgtg gtttaaaggt
     9361 gatatttttt ctcctgctga cagttggtat gtgtcacaaa caccgttaat caaaaccggc
     9421 ttactgaaag agattatttg taaagcactt cccctgcccg tagacgataa atcgttgagc
     9481 gaagtactgc atcaggttgg tcttgggaaa ttggctgcgc gtattcatga ccacgatcgc
     9541 tggggagata ttctttccag cggcgaaaaa caacgtatcg ccctggcacg attaatttta
     9601 cgacgtccga aatggatatt tcttgacgaa actacctctc atcttgagga acaagaggct
     9661 atccgcttac tgcgtttagt gcgtgaaaaa ctacccacaa gcggcgtcat tatggttaca
     9721 catcaacccg gtgtctggaa cctggccgat gatatttgtg acattagcgc ggttttataa
     9781 aaacaataaa agccctgctg tacggagata taaataaatg aagcgagttc ttattcctgg
     9841 cgtcatttta tgtggcgctg atgtggcgca ggccgtcgat gacaaaaaca tgtacatgca
     9901 tttttttgaa gagatgacgg tctatgctcc tgtccctgta cccgtaaacg gcaacacgca
     9961 ttacaccagt gaaagcatcg agcgtttacc gaccgggaat ggcaatatca gcgatctgct
    10021 gagaaccaac cctgcggtac gcatggattc aacgcaaagt acctcgttga accagggaga
    10081 tattcgccct gagaaaatct ctattcacgg tgcgtcgccc taccagaatg cctatttgat
    10141 tgacggtatt agtgcaacta ataacctgaa cccagcgaat gagtccgatg ccagtagtgc
    10201 aaccaatatt agcgggatgt cacaggggta ttatcttgat gtcagcttac tggacaatgt
    10261 gacgctttat gacagttttg tgccggttga atttggtcgc ttcaatggcg gggtaattga
    10321 tgcaaagatc aaacgcttca acgctgatga tagcaaggtg aaattgggtt atcgcactac
    10381 gcgttcggac tggttaacat cgcatatcga tgagaataac aagagcgcat ttaatcaagg
    10441 ttcttcagga agtacttatt tctctccaga ttttaaaaag aacttttata ccttgtcgtt
    10501 taatcaggag ctggctgata actttggcgt taccgccggt ttatcgcgcc gccagtctga
    10561 tatcacccgc gcggattatg tttcgaatga cggcattgtc gtcggtcggg cacagtataa
    10621 aaacgttatc gatactgcat tgagcaaatt tacctggttt gccagcgacc gctttaccca
    10681 cgatttaacc ttaaaatata ccggctccag ccgtgattat aataccagca ccttcccgca
    10741 gtctgatcgc gaaatgggta ataaatccta tggtcttgca tgggatatgg atacgcaact
    10801 cgcatgggcc aaactacgta ccaccgttgg ttgggatcat attagtgatt atacccgtca
    10861 cgatcatgac atctggtaca ccgaactttc atgtacatat ggtgatatta cagggcgttg
    10921 tacccgtggc ggattaggac acatttccca ggctgtagat aattacacct tcaaaacacg
    10981 cctggactgg caaaaattcg ccgtgggtga tgtttcgcat caaccctact ccggcgcgga
    11041 atacatctat tccgatgcat ggactgaacg ccataaccag tctgaatcct atgtgattaa
    11101 tgctgcagga aagaaaacta accataccat ttaccataaa ggtaaaggca gcctaggaat
    11161 tgacaactac acgctgtata tggcggatca cattagctgg cggaatgtgt cgttaatgcc
    11221 cggtgtgcgt tatgactatg acaactatct gtcaaaccac aatatctccc cgcgctttat
    11281 gacggaatgg gatatttttg ctgatcaaac ctcaatgatt accgccggtt ataaccgtta
    11341 ctatggcggg aatattcttg atatgggatt acgtgatatc cgcaatagct ggacggaatc
    11401 ggtatcaggt aataaaaccc tgacgcgtta tcagaatttg aaaacgcctt ataacgatga
    11461 actggcaatg ggattgcagc aaaaaatcgg taagaacgtt attgcacgcg caaactatgt
    11521 ttaccgtgaa gcgcatgatc aaatcagcaa aagcagtcgt accgacagcg cgactaaaac
    11581 caccattact gaatataaca acgatggcaa aaccaaaacg cattcgttta acctcagttt
    11641 tgaactggcc gaacccctgc atatccgcca ggtagatatt aacccgcaaa ttgtctttag
    11701 ctatatcaag agcaagggca acttgtcgtt aaacaatggt tatgaggaga gcaataccgg
    11761 tgataaccag gtggtttata acggtaatct ggtctcttac gatagcgttc cagtggcaga
    11821 ttttaataac ccattaaaga tctccttaaa catggatttc acgcatcaac cgagcgggtt
    11881 agtgtgggcg aatacgctgg cgtggcaaga agcgcgtaaa gctcgcatta tccttggtaa
    11941 gacaaatgcg caatacatca gcgaatattc agattacaag cagtatgttg acgaaaaact
    12001 ggatagcagc ctgacctggg acacccgctt gtcctggacg ccacaatttc tgaaacaaca
    12061 aaacctgacg atcagtgccg atattctcaa tgtactggat agcaaaaccg ctgttgatac
    12121 aacgaatacc ggtgtggcga cctacgccag tggccgtact ttctggcttg atgtcagcat
    12181 gaaattttaa tagttcggat tttacttaac cgggtaaatt cgcccggttt tcgcatggag
    12241 ataattatga gaaacctctg tttcttactg acgttagtgg caactctgtt gctccccggg
    12301 cggctgattg ccgccgcctt accgcaggat gaaaagttaa ttaccgggca actggacaat
    12361 ggcttgcgat atatgattta tccgcatgct caaccaaagg atcaggtaaa tttatggctg
    12421 caaattcata ccggttcatt gcaggaagaa gacaatgagc gcggcgtggc tcattttgta
    12481 gaacatatga tgtttaacgg cacaaaaaca tggccgggta ataaagtcat cgaaacattt
    12541 gagtcaatgg gcctgcgttt tggtcgcgat gttaatgcct ataccagcta tgacgaaacg
    12601 gtgtatcagg tgagtttgcc gactacgcag aaacaaaatc tgcaacaagt gatggcaatc
    12661 ttcagtgaat ggagtaatgc cgcaaccttt gaaaaactcg aagtagacgc tgaacgtggc
    12721 gtaattactg aggaatggcg tgcccatcag gatgcgaaat ggcgcacctc tcaggcgcgc
    12781 cgccctttcc tgctggctaa tacccgtaat ttagaccgtg aacctatcgg cctgatggat
    12841 actgtcgcca cggtcacacc ggcacaattg cgccaatttt atcaacgctg gtatcaacca
    12901 aataatatga cctttatcgt ggtcggcgat atcgacagta aaaaagcgct ggcgctgata
    12961 aaggataatt taagtaagct tccggctaac aaagcagctg aaaatcgcgt ctggccgaca
    13021 aaagccgaaa accacctgcg ctttaatatc attaatgata aagaaaaccg ggtgaacggc
    13081 atcgcactct attatcgcct gccaatggta caagtgaacg atgagcaaag ctttatcgaa
    13141 caagctgaat ggagcatgtt agttcagctg ttcaatcaac gtctgcagga acgcatacag
    13201 tcgggcgagt tgaagactat ttctggcggc actgcgcgca gcgttaaaat tgcacccgat
    13261 tatcagtcgc tgtttttccg tgtaaatgca cgagacgata atatgcagga tgctgcgaat
    13321 gcattaatgg cagagttggc aaccattgat cagcatggtt tttctgctga agaactcgat
    13381 gatgtcaaat ctacccgcct cacctggctg aaaaatgcgg ttgatcagca agctgaacgt
    13441 gatttacgta tgctgaccag tcgcctggca tccagctcat taaataatac gccgttcttg
    13501 tcgccggaag agacatatca actttcgaaa cgtctgtggc agcaaattac cgtgcaaagt
    13561 ctggcggaaa aatggcagca gttaagaaag aaccaggacg cattttggga gcaaatggta
    13621 aacaatgagc ttgccgccaa aaaagcattg tctcctgcgg ctatcctggc gctggaaaaa
    13681 gagtacgcca acaaaaagct ggcggcttac atcttcccag gcagaaattt atcgttaaca
    13741 gtagacgctg acccacaggc ggaaattagc agcaaagaaa cgctggctga gaatctgaca
    13801 tcattaacac tttccaatgg tgccagggtt attctggcaa aatccgcggg tgaagagcaa
    13861 aagctacaaa ttactgccgt atcgaataaa ggcgatttaa gtttccctgc gcagcaaaaa
    13921 tcacttatcg cgctggcaaa taaagcagta agcggaagcg gcgttggtga gctctcctct
    13981 tccagcctga aacgctggag tgcggaaaat tcggtaacca tgagcagtaa agtcagtggc
    14041 atgaatacgt tgctctccgt tagcgcgcgg actaataacc ctgaacctgg ttttcagttg
    14101 attaaccagc gaatcaccca cagcacgatt aacgacaata tttgggcatc gctacaaaat
    14161 gctcaaattc aggcgttgaa aacgctcgac cagcgtccag cggagaaatt cgcccagcag
    14221 atgtatgaga cgcgctatgc tgatgaccgc acgaaattac tgcaagaaaa tcagattgta
    14281 cagtttactg ccgcagatgc gctggctgcc gatcgccagt tgttttcatc tccagcggat
    14341 atcacgtttg tcattgtcgg taatgtctca gaagacaaac tcgtggcgtt aattacgcgt
    14401 tacttaggat caatcaaaca ctctgattcg ccattagccg caggtaaacc attaactcgc
    14461 gcgacggaca acgcatcggt tactgtaaaa gaacaaaatg aacctgtggc acaggtttca
    14521 cagtggaagc gttatgattc ccggacacct gttaatctgg cgacgcgtat ggcgctcgat
    14581 gcttttaacg tcgcactggc aaaagatcta cgtgttaata ttcgtgaaca ggcatctgga
    14641 gcatacagcg tttcttctcg cctctcggtt gatcctcagg ccaaagatat cagtcatttg
    14701 ctggctttta cttgtcaacc agaacgacat gatgaactgt taacgttggc gaatgaagtg
    14761 atggttaagc gtctggctaa agggatcagt gagcaagaac tgaatgaata ccagcaaaac
    14821 gttcagcgca gcctcgatat ccaacagcgt agcgttcaac aattagcgaa cactattgta
    14881 aatagtctta ttcaatatga cgatcctgca gcatggactg agcaggagca attgttgaaa
    14941 caaatgacgg tagagaatgt taacactgcc gttaaacaat atctttctca tccggtaaat
    15001 acttataccg gagtattatt gccaaaataa taacagcccc gtcaacacat cgttggcggg
    15061 gattttagca atattcgcta tttttatgta ataattttat aaatgcgttc aaaataataa
    15121 tcaagtacta atagtgatat tttaaggtct gatttttacg tgataattca ggagacacag
    15181 aatgcgcata aaaataacag cataaaacac cttaccacca cccaagaatt tcatattgta
    15241 ttgtttttca atgaaaaaac attattcgcg taatatctca cgataaataa cattaggatt
    15301 ttgttattta aacacgagtc ctttgcactt gcttacttta tcgataaatc ctactttttt
    15361 aatgcgatcc aatcatttta aggagtttaa aatggataag aagcaagtaa cggatttaag
    15421 gtcggaacta ctcgattcac gttttggtgc gaagtctatt tccactatcg cagaatcaaa
    15481 acgttttccg ctgcacgaaa tgcgcgacga tgtcgcattc cagattatca atgacgaatt
    15541 atatcttgat ggcaacgctc gtcagaacct ggccactttc tgccagacct gggacgacga
    15601 aaatgtccac aaattgatgg atttatccat taa
//
LOCUS       DAJTMC010000093        15613 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.093, whole genome shotgun sequence.
ACCESSION   DAJTMC010000093 DAJTMC010000000
VERSION     DAJTMC010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15613
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.093"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            1..604
                     /locus_tag="OO130_002593"
                     /pseudo
     CDS             1..604
                     /locus_tag="OO130_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490532.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            818..1966
                     /locus_tag="OO130_002594"
     CDS             818..1966
                     /locus_tag="OO130_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000570109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCR5365949.1"
                     /translation="MFEIDSLLLITSVIILSLLAVKLFDEISPIQLVDHGRNNQIDGM
                     RGFLAIFVLIHHAAIWHGYLLTGVWKTPSSNLLTNLGQVGVSFFFMITGYLFFSKIRS
                     SDQDWVRLYISRFLRLTPMFIVSLCLVLLVIGFKSRWSVHVSPASLTVSLMRWAPFTA
                     LGMPNINGVKDSFTINAAVTWTLVYEWFFYFSLPVIAALFKRRVSIHMIIISVLVLVI
                     FLCYPSKKIHVISFLFGLLASYSNNYVHRVAKAKITPIMIIALLSYEMIYFPTTYAPL
                     PLIICGVCFILIASGCDFYGILRLNLTRKLGETTYSIYLLHGVFLYCVMTWVIPSNYS
                     NYFFIMLISITAFCVTLLSCITFKLIELPFINITKQTAMKIRGIIKNT"
     gene            complement(2246..2857)
                     /locus_tag="OO130_002595"
     CDS             complement(2246..2857)
                     /locus_tag="OO130_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001057695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR5365950.1"
                     /translation="MNNAELNSELIATMAGEITVYNFDVMSREYISASTEYLAIGVGI
                     PAYSCLDAPGAYKAGYAICRSADFNSWEYVPDHRGEIVFSTETGESKEITAPGDYPDN
                     TTTIVPLTPYDKWDGEKWVTDTEAQHSAAVDAAEAQRQSLIDTAMASINLIQLKLQAG
                     RNLTQAETSRLNTVLDYIDAVTATDTSTAPDVIWPELLDAHNM"
     gene            complement(2857..3639)
                     /locus_tag="OO130_002596"
     CDS             complement(2857..3639)
                     /locus_tag="OO130_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HCR5365951.1"
                     /translation="MHRIDTKTAQKDKFGAGKNGFTRGNPQTGTPATDLDDDYFDMLQ
                     EELCSVVEASGASLEKGRHDQLLTALRALLLSRKNPFGDIKSDGTVKTALENLGLGEG
                     SALPVGVPVPWPSATPPTGWLKCNGAAFSAEEYPELAKAYPTNKLPDLRGEFIRGWDD
                     GRGVDNGRGLLTLQDGAIVSHNHYWGIWTSRTNDQTLESFTGTTILKQITPLSPSIDF
                     DNYPIPNPAITEGGVVAATTKPAGANETRPRNVAFNYIVRAA"
     gene            complement(3643..4227)
                     /locus_tag="OO130_002597"
     CDS             complement(3643..4227)
                     /locus_tag="OO130_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HCR5365952.1"
                     /translation="MDVTNDDYIRLLSALLPPGPAWSASDPAIAGAAPSLTRVHQRAD
                     ALMQELDPRTTTELINRWERLCGLPDECIPAGTQTLRQRQQRLDAKVNLAGGINEDFY
                     LAQLAALGRPDATITRYDKSTFTCSSACTDAVNAPEWRYYWQVNMPAATNTTWMTCGD
                     PCDSALRFWGDTVVECVLNKLCPSHTYVIFKYPE"
     gene            complement(4218..5276)
                     /locus_tag="OO130_002598"
     CDS             complement(4218..5276)
                     /locus_tag="OO130_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HCR5365953.1"
                     /translation="MADSEFQRPTLAENISMLRNDLFARLDVSDTLRRMDEDVRAKVY
                     AAALHTVYGYIDYLAMNMLPDLCDESWLARHAAMKRCPRKGATVASGYMRWEGVSDGL
                     KVTAGSVIQRDDLVQYTTTDDAISSGGVLRVPIACSSAGAVGNADDGTALILVTPVNG
                     LPSSGVADTLTGGFDTEELETWRARVIERYYWTPQGGADGDYVVWAKEVPGITRAWTY
                     RHWMGTGTVGVMIASSDLINPIPEESTETAARQHIGPLAPVAGSDLYVFRPVAHTVDF
                     HIRVTPDTPEIRAAITAELRSFLLRDGYPQGELKVSRISEAISGANGEYSHQLLAPAD
                     NITIAKNELAVLGAISWT"
     gene            complement(5263..5688)
                     /locus_tag="OO130_002599"
     CDS             complement(5263..5688)
                     /locus_tag="OO130_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HCR5365954.1"
                     /translation="MELWLTVNGKRTCASAPLDPLTRAVVISLFTWRRAEPDDNADVP
                     MGWWGDTWPAVQNDRYGSRLWLLQRSKLTNQLVQTVRGYIRECLQWMTDDGVVSRIDL
                     DIRRTGINELGNSITLRRRDGPVMISFDDLWSAITHGGQ"
     gene            complement(5688..6236)
                     /locus_tag="OO130_002600"
     CDS             complement(5688..6236)
                     /locus_tag="OO130_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein"
                     /protein_id="HCR5365955.1"
                     /translation="MRTIEAMQRQLLGLIGRAVVKSISAATKCQTVDVSLIAGEPKAG
                     VEHLEPYGFTARANSGAEAVVLFPDGDRSHAVVVTVSDRRYRLKGLQTGEVAVYDDQG
                     QSVTLTREGIVVDGAGKTITFRNAPEARFEMDLEVTGQVKDLCDSGGTTMSAMRLAYN
                     GHRHRENGQGSNTDKPDKAMEA"
     gene            complement(6236..7315)
                     /locus_tag="OO130_002601"
     CDS             complement(6236..7315)
                     /locus_tag="OO130_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate protein"
                     /protein_id="HCR5365956.1"
                     /translation="MNDNVTLRVNGREWNGWTSVRIGAGIERLARDFSVEITRQWPGD
                     EGITTLQPRIKNGSKVEVLIGDELVITGWVEATPVRYDARSVSTGIAGRSLTADLIDC
                     AAEPTQFNGRSLVQIAQALAAPFGIEVVNSGAPSGVIPDVQPDHGETVIEVINKILGQ
                     QQALAYDDPHGRLVIGGIGSTRAHTALVLGENILSCDTEKSIRERFSVYQVAGQRAGN
                     DDDFGEATTTALRARTEDAFIARYRPMYIRQTGQATGAGCIARADFEARKRAARTDET
                     TYVVQGWRQGNGTLWQPNQRVIVFDPVCGFDNTELLVSEVTFTQDQNGTLTEIRVGPP
                     DAYLPEPEAPGARKKKKARVQEDPF"
     gene            complement(7312..8640)
                     /locus_tag="OO130_002602"
     CDS             complement(7312..8640)
                     /locus_tag="OO130_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization N-terminal domain-containing
                     protein"
                     /protein_id="HCR5365957.1"
                     /translation="MTWKDRLQDASFRGVPFKVEEESAGTGRRVETHEYPNRDKPYTE
                     DLGKITFRPSITAYVVGDDCFDQRDRLIDALNKPGPGTLVHPTYGELKVCVDGEVRVS
                     TSKSEGRIVRFDLKFVEAGELSYPTSGAATAQTLMSSCSALDDCISDSFSGFSIDGVA
                     DFVQNDVVGNASTMLGYVSDAMKVVDSAVSDAARLLQGDISVLLPPPSSGKNFVEQVQ
                     KMWRTGKRLYGNASDLVTMIKTLSGVSLGSDLQPRGVWKTDSKTTATATQQRNVVAST
                     LRTTAISEAAYAVTRLPAPTTSAVMQNATVGQSTTPAQSTGWPSVTHPALNNAPAVKN
                     TVDLPTWEELTDIRDTLNTAIDKELSRTTSDALFLALRRVKADLNADINTRLEQSARI
                     IQRTPDEVLPALVLAATWFDNAARDADIIRRNAITHPGFVPVIPLKVPVQ"
     gene            complement(8701..10536)
                     /locus_tag="OO130_002603"
     CDS             complement(8701..10536)
                     /locus_tag="OO130_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR5365958.1"
                     /translation="MAEFELKALITGVDRLSPALSKMQKKIRGFKRQAEEASQGGLAL
                     GGGLAAGLTLSLKSYADQENAATGLKVAMMDANGEVGKSFQDINKLAIGLGNQLPGTT
                     ADFQNMMQMLVRQGIPAENILGGVGKATAYLAVQLKKTPEAAAEFAAKMQDATGTASE
                     DMMGLFDTIQKAFYLGVDDTNMLSFFTKTSSVLKMVNKDGLQAAQSLAPISVMMDQMG
                     MNGESAGNALRKVIQSGLSVKKIRDVNKIMARQKLGVQLDFTDGKGSFGGLDNMFRQL
                     AKLRKLTDVKRTGVLKAIFGDDAETLQVVNALIDKGKDGYDQIQQKMNKQASLNKRVQ
                     AQLGTLSNLWEAMTGTATNGLAAIGGAFSGDAKNITQWLGELGEKFTKFADENPRVIR
                     GVVGLAAGLAILKLGLMGVGGAISIVSRIMSMTPIGMIATAIALAAGLIITNWDVVGP
                     YFKKLWETIGPYFEAGWELLKKVFAWSPLGMVINNWGPVVKWFQDMWDKLKPIIEWFT
                     DSSGDTVDAINSAQWGAGAYDAYGTGIPARGYTPYQAVDPAQSNNASGATGPNPFMIN
                     KASAPKVDGEIKVSFVNSPPGMRVMETRSSGFDVSHDVGYTRFGR"
     gene            complement(10678..10947)
                     /locus_tag="OO130_002604"
     CDS             complement(10678..10947)
                     /locus_tag="OO130_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001526907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HCR5365959.1"
                     /translation="MKELELKKPITAHGETLSVLEFDEPTGKDVRELGYPYQMNQDES
                     VRLLAHVVSKYIVRLAKVPQSSVDQMSPADLNAAAWLVAGFFLQA"
     gene            complement(10947..11303)
                     /locus_tag="OO130_002605"
     CDS             complement(10947..11303)
                     /locus_tag="OO130_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HCR5365960.1"
                     /translation="MARIGGTCYFKIDGQQLSLTGGIEVPMNKTVNDDIIGLDGSVDR
                     KETHRAPYVKGTFKVPKNFPVSKITSSDEMTITAELANGQVYVLSSAWLHGEANHNAE
                     EGTVDLEFHGEEGDYQ"
     gene            complement(11303..12799)
                     /locus_tag="OO130_002606"
                     /pseudo
     CDS             complement(11303..12799)
                     /locus_tag="OO130_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
     gene            complement(12783..12953)
                     /locus_tag="OO130_002607"
     CDS             complement(12783..12953)
                     /locus_tag="OO130_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HCR5365961.1"
                     /translation="MFVKPVKGRSVPDPARGDLLPAEGRNVDENNYWLRREAAGDIRR
                     VNKKVNTDDDKL"
     gene            complement(12980..13522)
                     /locus_tag="OO130_002608"
     CDS             complement(12980..13522)
                     /locus_tag="OO130_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5365962.1"
                     /translation="MKLTPVIAALRARCPYFENRVAGAAQFKNLPEVGKLKLPAAYVV
                     PGDDSPGENKSQTDYWQELKEGFSVVVILSNGRDERGQFASYDVVDDVRQMLFKALLG
                     WNPEACGNPITYDGGTLLDLNRHELIYQFDFSVISELTEDDTRQQDDLNSLDELQTLA
                     IDVDYLEPGNGPDGDIEHHT"
     gene            complement(13519..14025)
                     /locus_tag="OO130_002609"
     CDS             complement(13519..14025)
                     /locus_tag="OO130_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5365963.1"
                     /translation="MATPFFHVDVQQPAEMRFNRARVRRAFVTIGQRHMRDARRLVMR
                     RARSAPGENPGYQTGRLARSIGYMVPRASKKRAGFMTRIAPNQRNGKGNRMISGDFYP
                     AFLFFGVRGGAKRRRSHHRGASGGSGWRLAPRNNFMVETLEKNRSWTRYFLARELRKS
                     LKPERRHR"
     gene            complement(14000..14410)
                     /locus_tag="OO130_002610"
     CDS             complement(14000..14410)
                     /locus_tag="OO130_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HCR5365964.1"
                     /translation="MKIRQAQTSATYILPDPGELNKRVLIRQRVDMPADNFGVEPQYP
                     VTFRTWAKVIQTSATTWQETAQTGDAITHYITIRYRRGITADYEVVCDDSVYRVKRQR
                     DLNGARRFLLLECTELGECRQSHGGSNGDSLFSR"
     gene            complement(14407..14730)
                     /locus_tag="OO130_002611"
     CDS             complement(14407..14730)
                     /locus_tag="OO130_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001492759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HCR5365965.1"
                     /translation="MLLTMEEIKAQLRLDEDFDADDRHLQLLACAAQKRTETYLNRKL
                     YAPDETIPDSDPDGLHLPDDIRLGMLMLISHFYENRSSVTEVEKLDMPQSFGWLVGPY
                     RYFPQ"
     gene            complement(14733..14933)
                     /locus_tag="OO130_002612"
     CDS             complement(14733..14933)
                     /locus_tag="OO130_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001735262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5365966.1"
                     /translation="MILKQDLKWSPDGMRVEVIRAGEYDDGALPARVQEIALQAGLAE
                     RGTSAKSSKATKEKKATTSKEG"
     gene            complement(14982..>15613)
                     /locus_tag="OO130_002613"
     CDS             complement(14982..>15613)
                     /locus_tag="OO130_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HCR5365967.1"
                     /translation="PQATQKMLDDAFFNVEAWINSELATEFAEQEEIAFTTGDGTKKP
                     KGFLANESTDETDKVRAFGKLQHIVSGDATAVTADAIIKLIYTLRKAHRTGAKFMMNN
                     NSLFAIRLLKDSEGNYLWRPGLELGQPSSLAGYGIAENEQMPDIAADAKAIAFGNFKR
                     GYTIVDRIGTRILRDPYTNKPFVGFYTTKRTGGMLVDSQAIKLLKIAVA"
BASE COUNT         3586 a         4436 c         3764 g         3827 t
ORIGIN      
        1 gcccgcacca acgctgaaaa tcaaagaccc gcactatgat gaaaagcacc ttgctattga
       61 gcaggcgctg actcaggggc agacgaaaca tcctgtgttt tatcaggatg aagtcgacat
      121 cgacctgaat acgaaaatcg gcgtggactg gatgcccaaa gggcaacaga aacgcatcgc
      181 tacgccggga cagaacctga agcattatct ggctggcgca ctgcactcag gtacgggacg
      241 ggttcactag gccagtggca gcagcaaaag ttctgattta tttatcagtc tgttagaggc
      301 attacggcgt agatatcggc gggcgaaaac catcacactg gtggcggata actacatcat
      361 ccataaaggc cgtaaagtgg agcgatggct ggaagaaaac ccgaagttcc ggttgctgtt
      421 cctgccgacg tattcaccgt ggctgaatcc gatagagcaa ctttggttat cgttacacaa
      481 gaccataacg cgaaatcatc agtgccggta tatgtggcag atactgaaac aggtaacgca
      541 gtttatgaat gctgctttac cgtttccggg taatcaacac ggtatggcca aagtggagcg
      601 gtaatatgcg aagctattta gattcagttt gaatctcagc cctgacattc attctttacc
      661 gacgttccat tagcccaaat ctgagtatat aacctcaatt caactgacta aatttaacac
      721 aatatcaatg acaaatgtct ttaccattgg tacatacaat tgcaaaatca ccatctctaa
      781 gcaataagac tacgcatatt aaagtgagtt atattaaatg tttgaaattg atagcctatt
      841 attaataaca tccgtgataa tcttgtcatt attggctgta aaattatttg atgaaatttc
      901 tcctatacaa cttgtcgatc atggaagaaa taatcagata gatggaatga gaggattctt
      961 agccatcttc gtacttattc atcatgccgc tatttggcat ggctacttat taaccggggt
     1021 atggaaaact ccgtcatcaa acttattaac aaatttaggc caagttggtg tctcattctt
     1081 tttcatgatc accggatatc tatttttttc gaagattcgc tcaagtgacc aggattgggt
     1141 aagactatat atatcgagat ttctgcgatt aactcctatg tttattgtaa gtttatgttt
     1201 agttcttctt gttataggtt ttaaatcaag atggagcgta catgtctccc ctgcctctct
     1261 tactgtgtca ctcatgagat gggcaccatt caccgcatta gggatgccaa atattaacgg
     1321 tgttaaggac tcattcacta tcaatgcagc tgtaacatgg accctcgttt acgaatggtt
     1381 tttttatttt tcattaccgg ttatagctgc actcttcaaa agaagagtca gcatccacat
     1441 gattataata agtgttttag ttctagtgat tttcttatgt tacccatcaa aaaaaataca
     1501 tgttatttca tttttatttg gactgttagc ctcttattca aataattatg tacatagagt
     1561 tgctaaagct aaaataacgc ctattatgat aattgcatta ctaagttacg aaatgatata
     1621 ttttcctaca acctacgctc cactccctct cataatttgc ggtgtttgct ttatacttat
     1681 cgcatcaggc tgtgattttt atggtatatt gagattaaat ttaacaagaa aacttggtga
     1741 aacaacatat agtatttatc ttttacatgg agtattcctt tattgtgtaa tgacatgggt
     1801 catccctagt aattattcta attatttttt tattatgtta ataagcatta ctgctttttg
     1861 cgtcacactt ttatcatgca taacatttaa attaattgaa ctacctttca ttaatataac
     1921 aaaacaaacc gctatgaaaa tcaggggcat aataaaaaac acttagtaat caattgtcac
     1981 ctaatcaaga ggtggtttat agataacaat aaagaacctt cgccatttcc ggaggttctt
     2041 ttatatcctt atggtaaata taaattataa atattatttt tatggtgttc cactatgaac
     2101 agggaagcta aataatgatt acaaaaatat gtaacttcag gcaatgcagg ggctatgtta
     2161 ttgccttaac accataatga gtaatattgt gtcccgagga taaaagtact gttaattctt
     2221 attgatgctt caatagcact caaaatcaca tattatgtgc atccaacaac tcaggccaga
     2281 tgacatccgg cgcggtgctg gtatctgttg ccgtcaccgc gtcaatgtaa tccagcaccg
     2341 tgttaagtcg gctggtttct gcctgcgtca gattccgtcc ggcctgtaat tttagttgaa
     2401 tcagattgat ggaagccatt gcagtatcaa tcagcgactg gcgttgtgct tctgctgcat
     2461 cgactgcggc gctatgttgt gcctcggtat ccgtcaccca tttctcacca tcccatttat
     2521 cgtatggtgt taacgggacg atagtggttg tattatcagg gtaatcaccc ggagctgtga
     2581 tttcttttga ttctcctgtt tcggtgctaa agacgatttc accgcgatgg tctggcacat
     2641 attcccatga gttaaaatct gcagaacggc agattgcata accagctttg tatgcgcctg
     2701 gagcatctaa acaggaatat gccggaatgc cgacaccaat agcaagatat tcagttgaag
     2761 ctgaaatata ctcccgactc atgacatcaa agttataaac agtaatttct cctgccatag
     2821 tggcaattaa ttcactgttt aattctgcgt tattcattat gcagccctca caatatagtt
     2881 aaaagcgaca tttcgtgggc gtgtttcatt cgcacctgca ggtttagtcg ttgccgcaac
     2941 aacaccaccc tctgtaatag ccgggttggg aattgggtaa ttatcgaagt caatggacgg
     3001 agacaggggc gtgatttgtt ttaaaatcgt ggtgcctgta aaactttcca gagtctggtc
     3061 gttagttcgt gaagtccaga ttccccaata gtggttatga ctgacaatag caccgtcctg
     3121 aagcgttaat aatccccttc cgttatccac tccacgcccg tcatcccagc cccgaataaa
     3181 ctcaccgcgt aaatcaggta atttatttgt cggataagcc tttgccagtt ccgggtattc
     3241 ttcagcagaa aaagctgctc cgttgcattt cagccagcct gttggcggag tggctgaagg
     3301 ccacggaaca ggcacaccaa cgggtaatgc tgagccttct cccaaaccaa ggttttcgag
     3361 agctgttttc accgtgccat ccgatttgat atcgccaaac ggattcttgc ggcttaacag
     3421 cagcgcacga agcgcggtaa gcagctggtc atgccgcccc ttctccaggc tggcaccgga
     3481 tgcctccaca acgctgcaaa gctcctcctg caacatgtca aagtagtcat catccagatc
     3541 ggtggcaggc gtgccggtct gggggttacc acgggtaaaa ccgttcttac ccgcgccgaa
     3601 cttatccttc tgcgcggttt tcgtgtctat acgatgcatg gattactccg gatatttaaa
     3661 aatcacatag gtatgcgacg ggcagagttt gttaagaaca cactcgacaa cggtgtcccc
     3721 ccagaagcgc agcgcggaat cacagggatc gccacatgtc atccaggtgg tgttggtggc
     3781 ggctggcatg ttgacctgcc agtaataccg ccattccggc gcattcaccg cgtcagtaca
     3841 ggcagatgag caggtgaacg tgcttttgtc gtatcgcgtg atggtggcgt ctggtctgcc
     3901 caaggcagca agctgtgcaa ggtaaaaatc ctcattgatg ccgcccgcca ggttaacctt
     3961 cgcatccagc cgttgctgac gctggcgaag ggtctgcgtc cctgccggaa tacattcatc
     4021 cggcaggccg cacagacgct cccagcggtt tatcagttcg gtggtggtgc gcggatccag
     4081 ctcctgcatc agggcatccg cacgctgatg aacacgggtt aatgacggtg ccgcaccggc
     4141 aatcgccgga tcgctggctg accacgccgg accgggcggc aacagtgctg acaacagacg
     4201 gatgtaatca tcgtttgtca cgtccatgaa atcgccccca gaaccgccag ttcatttttc
     4261 gcaatggtga tattgtccgc cggtgcaagc aactgatggc tgtattcccc gttcgcaccg
     4321 gaaatcgctt cactgatacg tgacaccttc agttctccct gcggataacc atcacgcagc
     4381 aggaacgaac gcaactccgc ggtgatggca gcccgtattt ccggtgtgtc cggcgtcaca
     4441 cggatatgaa aatccaccgt atgtgccacc ggcctgaaca catacaaatc agagcctgcc
     4501 accggggcca gtggcccgat atgttgtctt gccgccgttt ccgttgattc ttccggaatg
     4561 ggattaatca ggtcactgct ggcaatcatc acaccgacag tccccgttcc catccagtgt
     4621 cggtatgtcc atgcgcgggt aatgccaggc acttctttag cccagacgac atagtccccg
     4681 tcagccccgc cctgcggcgt ccagtaatac cgctcaatga cgcgggcgcg ccacgtttcc
     4741 agctcttcag tatcaaatcc gcctgtcagg gtgtcagcca caccggaaga cggcagacca
     4801 ttcaccggcg tgaccaggat taatgccgta ccgtcgtcag cgttaccgac cgcacctgca
     4861 cttgagcagg cgatcggcac gcgcaggaca ccaccggagc tgattgcatc gtcagttgtc
     4921 gtgtactgca ccaggtcatc gcgctgaata acactcccgg cagtcacctt caggccatcg
     4981 ctgacacctt cccagcgcat atacccgctg gcaaccgtgg cccccttgcg cggacaccgt
     5041 ttcatcgcag catgtcgcgc cagccaggac tcatcgcaca ggtcaggcag catattcatt
     5101 gccagataat cgatgtaacc gtagaccgta tgcagcgccg ccgcatacac ctttgcccgc
     5161 acgtcttcat ccatgcgccg gagcgtgtcg ctgacgtcca gcctggcgaa taaatcgtta
     5221 cggagcatac tgatattttc tgccagcgtc gggcgctgaa attcactgtc cgccatgcgt
     5281 tatcgcactc cacagatcat caaaagaaat cattactggt ccatcacgac gcctgagggt
     5341 gatactgtta cccagctcat taatcccggt gcggcggata tccagatcaa tacgggacac
     5401 cacgccgtca tcagtcatcc attgcaggca ttcgcggata tatcccctta ccgtctgcac
     5461 cagctgattg gtcagtttgc tgcgctgaag caaccacagt cgggagccgt aacggtcatt
     5521 ctgtaccgca ggccaggtat ccccccacca tcccatcggg acgtcggcat tgtcatcagg
     5581 ctccgcccgc cgccaggtga acagggaaat caccacggcg cgggtcagcg gatccagcgg
     5641 tgcgctggcg caggtgcgtt taccgttcac cgtcagccac agttccatca tgcctccatc
     5701 gctttatcag gtttgtcggt gttactgccc tgaccgttct ctctgtgacg atgcccgtta
     5761 taggcaagcc gcatcgctga catggtggtg ccgccggagt cgcacaggtc tttcacctgt
     5821 ccggtcactt ccagatccat ttcaaaacgt gcttcaggtg cattgcgaaa cgtgatcgtt
     5881 ttccctgcac cgtccaccac gatcccctcc cgggtcagcg tcacagactg cccctgatcg
     5941 tcatagacag ccacctcacc cgtttgcagc cctttcaggc ggtagcgccg gtccgacacc
     6001 gtaacaacca ccgcatgaga acggtcgcca tccggaaaca acaccaccgc ttccgcaccg
     6061 ctgtttgccc ttgcggtaaa accgtagggt tcaagatgtt caaccccggc tttgggttca
     6121 ccggcaatca gggacacatc cacggtctga catttcgtgg cggcactgat gcttttcacc
     6181 actgcccgcc caatcaggcc gagaagttgt cgctgcatgg cttcaatcgt cctcatcaga
     6241 acgggtcctc ctgtactctg gcttttttct ttttccgcgc gccgggggct tcgggttcag
     6301 gcagataagc gtcaggcggg ccgacacgga tttccgtcag ggtgccgttc tggtcctgag
     6361 taaacgtgac ttccgagaca agcagttcgg tattgtcgaa accacagacc ggatcgaaga
     6421 caatcacccg ctggttgggc tgccacagcg taccgttacc ctgtcgccag ccctgcacca
     6481 cataggtggt ttcatccgtc cgcgccgccc gttttcgggc ttcaaagtca gcacgcgcaa
     6541 tacagcctgc ccccgtggcc tgccctgtct gcctgatata catcggacgg taacgggcaa
     6601 taaatgcgtc ctctgtgcgg gcccgcagcg cggtggtggt ggcctcaccg aaatcatcgt
     6661 cgtttccggc acgctgcccc gccacctggt aaacagaaaa ccgctcccgg atactcttct
     6721 ccgtatcaca ggaaaggatg ttttccccaa gtaccagcgc ggtatgtgcc cgcgttgagc
     6781 caataccgcc aatcaccagc ctgccgtgcg ggtcgtcgta agccagcgcc tgctgctgac
     6841 cgagtatttt gttgattacc tcaatcaccg tttcaccgtg atcaggctga acatcaggaa
     6901 taacacccga cggcgcaccg ctgttcacca cctcaatgcc gaaaggcgca gcaagcgcct
     6961 gcgcaatctg caccagcgag cgtccgttaa actgtgtcgg ttcggctgca cagtcaatca
     7021 ggtcagccgt cagactacgt ccggcaatac cggtgctgac cgaacgggca tcgtaacgaa
     7081 cgggagtcgc ctccacccag ccggtgatca ccagctcatc accaatcagc acttccactt
     7141 ttgaaccgtt tttaatgcgc ggctgaagcg tggtgatacc ctcatctccc ggccattggc
     7201 gagtgatctc cacactgaaa tcccgcgcca gccgttcaat accggcaccg atgcgcaccg
     7261 atgtccagcc attccactcc cggccattta cccgtagcgt gacattgtcg ttcattgcac
     7321 tggcaccttc agagggatca ccggcacaaa gccgggatgc gtaatggcat tacgccgaat
     7381 aatgtccgcg tcacgcgccg cgttatcaaa ccaggtcgcc gccagcacca gcgcgggtaa
     7441 aacctcatcc ggcgtgcgct gaatgatccg tgcagactgt tcaaggcgcg tgttgatatc
     7501 cgcattcaga tctgctttca cccggcgcag cgccagaaac agcgcatcac tggttgtacg
     7561 ggacaactcc ttatcaattg ccgtattcag tgtgtcgcga atgtcggtca gttcttccca
     7621 cgttggcagg tcaaccgtgt ttttcaccgc cggtgcattg ttcagtgccg gatgcgtgac
     7681 agaaggccag ccggtgctct gcgcgggtgt tgttgactgc cctactgtgg cattctgcat
     7741 caccgcggaa gttgtgggcg caggcaatcg tgtgacggca tacgccgctt cgctgattgc
     7801 ggtcgtacga agggtgctgg caaccacgtt acgctgctgc gtcgccgtgg cggtagtttt
     7861 actgtccgtt ttccagacgc cgcgcggttg cagatcgctg ccgaggctga caccggaaag
     7921 cgttttgatc atggtgacca ggtcgctggc gttaccataa aggcgtttcc cggtacgcca
     7981 cattttctgc acctgctcaa cgaaattttt gcctgacgat ggtggcggca gaagtaccga
     8041 gatatccccc tgcaacagcc tggcggcatc cgatacggca gaatccacca ctttcatcgc
     8101 atcagaaaca tacccaagca ttgtgctggc attaccgacg acgtcgttct gcacaaaatc
     8161 tgccacgcca tcgatactga aaccactgaa gctgtcactg atgcagtcat ccagtgcaga
     8221 acaggatgac atcagcgtct gcgccgtcgc cgcacctgaa gtggggtaag agagttctcc
     8281 cgcttcgaca aacttcaggt caaagcggac aatacgccct tcactcttcg atgtgctgac
     8341 ccgaacttcc ccgtcaacac agactttcag ctcaccgtaa gtcggatgga caagcgtgcc
     8401 gggaccgggt ttattcagcg cgtcaatcag gcgatcgcgc tggtcaaagc agtcatctcc
     8461 caccacataa gctgtgatgg acgggcggaa agtgattttc cccaggtctt cggtataggg
     8521 tttgtcgcgg ttcgggtatt cgtgcgtttc cacacgacga ccggttcccg cactttcttc
     8581 ttcaacctta aacggcacac cgcgaaatga cgcgtcctga agtctgtctt tccacgtcat
     8641 ataaactccg tacataaaaa atcccaccgg agtgggactc attaacagat taatttttca
     8701 ttacctgcca aagcgcgtat agccaacatc atggctgaca tcaaaaccgc tggatcgcgt
     8761 ttccataacc cgcatacccg gaggcgaatt cacaaaagag accttgatct caccatcaac
     8821 ttttggcgca gaagctttgt taatcatgaa gggattcggg cctgtggcac cggaggcgtt
     8881 gtttgactga gccggatcta ccgcctgata aggtgtgtat ccccgtgccg gtattcccgt
     8941 cccataagca tcataagcac ccgcgcccca ctgcgcagag ttaatggcat cgaccgtgtc
     9001 accggaactg tcggtaaacc actcaataat tggcttcagt ttgtcccaca tatcctgaaa
     9061 ccacttaaca accggtcccc agttattgat taccatcccc agcggcgacc aggcaaaaac
     9121 cttcttcaga agttcccagc cagcctcaaa ataaggacca atggtttccc agagcttctt
     9181 gaaataaggt ccgacaacat cccagttagt gataattaat cccgcagcca gggctatcgc
     9241 cgtcgcaatc attccaatcg gcgtcatcga catgatcctg ctgacaatac tgatggcacc
     9301 gccaacgccc atcaatccca gtttcagaat cgcaagaccg gcagcaagcc cgacgacgcc
     9361 gcgaataacc cggggatttt catccgcaaa cttcgtgaat ttttccccta actcccccag
     9421 ccattgcgtg atatttttgg cgtcaccaga aaatgcgccg ccaatagccg caaggccgtt
     9481 agttgcggtc cccgtcattg cctcccacag gttggacagc gtaccaagct gggcctgaac
     9541 acgtttattc aggctggcct gtttattcat cttctgctgg atctgatcgt agccatcctt
     9601 tcctttatcg attagtgcat tgaccacctg aagggtttcg gcatcatcac caaatattgc
     9661 cttaagtaca cctgttcgct taacgtcggt cagttttcgc agctttgcca gttgcctgaa
     9721 catgttatca agaccgccaa aacttccttt gccgtcagta aaatcgagct gtaccccgag
     9781 tttctggcgg gccatgattt tattgacgtc cctgattttc ttaacgctta atccggactg
     9841 gataactttt cgcagggcgt tacctgccga ctccccgttc atccccatct gatccatcat
     9901 gacgctgatg ggggcaaggc tctgtgcagc ctgaagaccg tccttgttca ccatcttcag
     9961 aacagagctg gttttagtga agaaggacaa catgttggta tcgtcaacac ccagataaaa
    10021 cgccttctgg atagtgtcga acagccccat catgtcttct gacgccgttc cggtagcatc
    10081 ctgcatcttt gcagcaaact cagcagccgc ttccggtgtt tttttcagtt gtaccgcaag
    10141 ataagctgtc gctttaccca caccgccaag aatgttttct gccgggatcc cctgacgcac
    10201 cagcatctgc atcatgttct ggaaatcagc cgttgtaccg ggtagctggt tacccaggcc
    10261 aatagccagt ttattgatgt cctgaaagct ctttccgacc tcgccgttcg catccatcat
    10321 ggcgactttc agcccggtgg cggcgttttc ctgatcggca taagatttca gggaaagcgt
    10381 cagacccgct gccagtccgc caccaagcgc cagcccaccc tgtgacgctt cttccgcctg
    10441 gcgtttaaat ccccggattt tcttttgcat tttcgacagc gcgggagaaa gcctgtcgac
    10501 accggtgatc aacgccttaa gctcaaattc cgccatgtgt gcgtttctcc tgctctatcc
    10561 tgtttgcctg actgaccagc aaggtaattt cactgatcgg catattcagc aattcgaaag
    10621 gattaatgcg ccagtaactg gcgcagtcaa agaagcgatc agtgaggtat tcagccgtca
    10681 ggcctggagg aaaaaaccag ccacaagcca cgccgctgca ttcaggtctg ccggagacat
    10741 ctggtcgaca gagctttgcg gcactttcgc cagccgcaca atgtatttcg acaccacatg
    10801 cgccagaagt ctgactgact catcctgatt catctggtag ggatacccca gctcgcggac
    10861 atccttcccg gtgggttcat caaactccag tacggagagt gtctcgccat gagcagtaat
    10921 cggtttcttt aactcaagct ctttcattac tggtaatccc cttcttcacc gtggaactca
    10981 agatcaaccg tgccttcttc ggcattatgg ttcgcttcgc cgtgcagcca ggcagacgac
    11041 agtacataga cctgaccgtt cgccagctcg gcagtgatgg tcatctcatc agacgaggtg
    11101 attttgctta ccggaaaatt cttcggcacc ttgaaggtcc ctttgacata aggcgcacgg
    11161 tgagtttcct tgcggtccac tgaaccgtcc aggccgatga tgtcatcgtt aaccgttttg
    11221 ttcatgggca cctcaatgcc gccggtcagc gatagctgct gaccgtcaat tttgaaataa
    11281 caggttcccc cgatacgggc cattatgcag actcctctga atactgaaga cggaactgat
    11341 taaccacggc aaagacacgc agctggttaa catagtcagg cgggaacagc gtgttcaggc
    11401 ggttcggatc gctggcatca cgctccacaa ccaggtactg cttaaacagt tcgtagtttt
    11461 ccacgatccc cgcacgctca agctgacggt aggttgccag cagttcccct ttgatcaccg
    11521 ccggggtgac aatcgcctga ccgggaccaa agcgggtacc gtcactggca agcttgtgac
    11581 gcccgtactt actggtaatg acggatttca gtttgcgcag tacatacgcg ctggtatgca
    11641 gcgtctcact gtcgaggtaa ctgttatccg caaccccgta agcgtttttc ctgtacgtgg
    11701 tgacatcacg ctgaatgcgt agtaccccgc tttcgacata cgccgttgcc acgccatgag
    11761 acagcagggt ctgttgttcg gtcatcgtga accgtttccc cttcggcgca ggcagcatac
    11821 ccaccagctc accggtctgc gtgggacgtg ccggatcgtt gcggataaac accgctgcgc
    11881 gggcggtacg gcttgccgcc agctcgtcgg caggcgtctg ggtctctttt tcgtaccccg
    11941 ccagggtaat gtgctgctgg ttaaactggt cacctgcggt caccagttct gacagcgtgc
    12001 cgatctttgc cgtatacaca tgaccataca gctgacgcgc atagctccag cgaccgctgg
    12061 tatcgttcat ctcggtcacc agcgtgttaa cggaggccgt gtcgttgaac ggcaggccga
    12121 tataatcaaa cggctcatcc gccattgcag ccaccgcgcc ggtgagaacc ggagcacccg
    12181 ttccggcggt acccgtcgcc acggcaatct gtacgcccgc tggcagcact tcgcccccac
    12241 caaagccgta gtaattgagg ctgacaggaa tttcattccc acaaagcccc ttatgacgcg
    12301 cggtcagtgt gaccacgcct gccgaagatg aagccgtaaa cggcagggtc ggaacggcat
    12361 tgatggcatc ctagatactg ctggcaatca tcgtgacgtt atcgccgtta gtcaccggtg
    12421 cctgcacgcg ggtacgtcct acatacacat tcaccgtgcc ggtttcggtt gccgccccgg
    12481 tcaccgtcag cgtaactgtt gccgccgcac ctgtggattc aggaacggca atcacataca
    12541 gctcgccaaa cgggtcagtc tggcgataag cctcgaccat acgcgccagc tgacttcccg
    12601 caccacaaat ctggcgtgca tagtctgccg acgacatcag taccagactg ttggcaacaa
    12661 tctctgcacc gttattggca tgaccaatca gcagcgatgc tccgctgtcc tgtgcagtat
    12721 tcgccgcctg gttatccatt tccgcataaa acaacggaac cagcgtattc gacggaatgg
    12781 tgttaaagct tatcgtcatc ggtgttcacc tttttattca cgcgccggat atcacccgct
    12841 gcttcacggc gcagccagta gttgttctcg tcaacatttc gcccttcggc gggcaaaagg
    12901 tcgccgcggg cagggtcagg cactgaccgc cctttaacag gtttgacaaa catgaggatc
    12961 ctcaggaagg aagggttatt taggtgtgat gttcgatatc gccgtcaggc ccgttaccgg
    13021 gctcgagata atcaacatca atcgccagcg tttgcagttc atccagactg ttcagatcat
    13081 cctgctggcg ggtatcgtct tcagtcagct cgctgatgac cgaaaaatcg aactgataaa
    13141 tcagctcatg acgattcaga tccagcagcg tgccgccgtc ataggtaatc gggttaccgc
    13201 acgcctccgg gttccagccc agcagagcct taaagagcat ctgccggaca tcgtccacca
    13261 catcatacga ggcaaactga ccacgctcat cacgcccatt actcagtatg acaaccacgg
    13321 agaaaccctc tttcagctcc tgccagtagt cggtctggct tttgttttct cccggagaat
    13381 catcacccgg tacaacatat gccgccggga gtttcagctt tccgacctcc ggcagatttt
    13441 tgaactgggc cgcgcctgca acccggtttt caaaatacgg acaacgggca cgcagtgcag
    13501 caataacagg agtcagtttc atctgtgtcg tcgctccggc ttcagtgatt tacgcaattc
    13561 ccgcgccaga aaatagcgtg tccagctgcg gttcttttca agcgtttcca ccatgaagtt
    13621 attacgtgga gccagtcgcc agccgctgcc accggatgca ccacgatgat gactacgacg
    13681 acgttttgct cctccccgga caccaaaaaa cagaaacgcc ggatagaagt caccagagat
    13741 catccggttc cccttcccgt tgcgctggtt aggggcaatg cgtgtcataa aaccggctcg
    13801 ctttttactg gctctcggca ccatataacc aatcgaacga gccaggcgtc cggtctgata
    13861 accggggttt tcacccggtg ccgaccgcgc acggcgcatc accagccgac gggcatcacg
    13921 catatgacgc tgcccaatcg tgacaaacgc ccgccggaca cgagcgcggt taaagcgcat
    13981 ctcggcgggc tgctgaacat caacgtgaaa aaagggagtc gccattgctg cctccgtgac
    14041 tctgcctaca ttcgcccagc tccgtacact ccagcagcag aaagcgccgc gccccgttca
    14101 gatcacgttg acgtttcacc cggtacacac tgtcatcaca gaccacctca taatcagcag
    14161 tgatcccccg gcggtaacga atggtgatgt aatgggtgat ggcgtccccg gtctgcgcgg
    14221 tttcctgcca ggtggtggca ctggtctgga taaccttcgc ccatgtccgg aacgtaaccg
    14281 ggtattgagg ctccacgcca aagttatccg cgggcatatc cacccgctgg cggatcagga
    14341 cgcgtttatt cagttcaccg gggtccggca gaatgtaggt tgcgctggtc tgcgcctgac
    14401 gaattttcat tgcggaaagt acctgtacgg gccgacaagc cagccaaaac tctgcggcat
    14461 gtcgagtttc tccacttccg taaccgacga gcggttttcg taaaaatggc tgataagcat
    14521 cagcatcccc agacgaatat catccggcag gtgcagcccg tccggatcgc tgtccggaat
    14581 ggtttcatcc ggtgcataga gcttccggtt cagatacgtt tccgtccgct tttgtgccgc
    14641 acaggccagc agttgcagat ggcggtcatc agcatcgaaa tcctcatcca gccggagttg
    14701 ggctttaatc tcttccattg tcagaagcat actcagccct ctttactggt cgtggctttt
    14761 ttctcttttg tcgctttact gctttttgca ctggttccgc gctctgctaa cccggcctga
    14821 agtgcaatct cctgcacccg ggcaggaagc gccccgtcgt catactcacc ggcccgaatg
    14881 acctcaacac gcataccgtc cggtgaccat ttcagatctt gtttcaggat catgattctt
    14941 cacccgtcag aacaggggcg cggttccgcg cccctgagtg attacgccac tgcaatcttc
    15001 agcagtttga tggcctgcga atcgaccagc atgccgccgg tgcgtttggt ggtataaaaa
    15061 ccgacaaacg gtttattggt gtacgggtca cgcagaatgc gggtgccgat acggtcaacg
    15121 atggtgtaac cccgtttgaa gttaccaaat gcaatggctt tcgcatcagc ggcgatatcc
    15181 ggcatctgtt cgttttcagc gataccgtaa cccgccagag aggacggctg ccccagctcc
    15241 agccccggac gccacagata gttaccctcg ctgtctttca gcagacggat ggcaaacagg
    15301 ctattgttgt tcatcatgaa cttcgcgccg gtgcggtgtg ccttacgcag cgtgtaaatc
    15361 agtttgataa tggcgtctgc ggtcaccgcc gtcgcgtcgc cggatacaat atgctgaagt
    15421 ttgccgaacg cccggacctt atcggtttca tccgtggatt cattcgccag gaaccctttc
    15481 ggcttcttgg taccatcgcc ggtggtaaag gcaatttctt cctgttcggc aaattcggtt
    15541 gccagctcgc tgttgatcca tgcttccacg ttgaaaaagg catcatccag cattttctgg
    15601 gtggcctgcg ggt
//
LOCUS       DAJTMC010000094        15312 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.094, whole genome shotgun sequence.
ACCESSION   DAJTMC010000094 DAJTMC010000000
VERSION     DAJTMC010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15312
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.094"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(<1..321)
                     /locus_tag="OO130_002614"
     CDS             complement(<1..321)
                     /locus_tag="OO130_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HCR5365968.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTDINAERKASYQQLAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVR"
     gene            complement(326..511)
                     /locus_tag="OO130_002615"
     CDS             complement(326..511)
                     /locus_tag="OO130_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HCR5365969.1"
                     /translation="MKILLAALTSSFMLAACTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLY"
     gene            complement(508..3147)
                     /locus_tag="OO130_002616"
     CDS             complement(508..3147)
                     /locus_tag="OO130_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HCR5365970.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVIAFEYQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALISGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLTDPTLAFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTNINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            3355..4344
                     /gene="ldhA"
                     /locus_tag="OO130_002617"
     CDS             3355..4344
                     /gene="ldhA"
                     /locus_tag="OO130_002617"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCR5365971.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPANYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            4455..4877
                     /gene="hslJ"
                     /locus_tag="OO130_002618"
     CDS             4455..4877
                     /gene="hslJ"
                     /locus_tag="OO130_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HCR5365972.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(4874..5140)
                     /locus_tag="OO130_002619"
     CDS             complement(4874..5140)
                     /locus_tag="OO130_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HCR5365973.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            5414..8938
                     /gene="nifJ"
                     /locus_tag="OO130_002620"
     CDS             5414..8938
                     /gene="nifJ"
                     /locus_tag="OO130_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HCR5365974.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DSYFQSREATNPWYNAVYDHVEQAMNDFSAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQTLPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGKRETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTQPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVEGNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
     gene            9304..10437
                     /gene="ompN"
                     /locus_tag="OO130_002621"
     CDS             9304..10437
                     /gene="ompN"
                     /locus_tag="OO130_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HCR5365975.1"
                     /translation="MKSKVLALLIPALLAAGAAHAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNSAKDGDQSYARLGFKGETQINDQLTGYGQWEYNIQANNTESSKNQSWTRLAFAGL
                     KFADYGSFDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFF
                     GLVNGLNFAVQYQGNNEGASNGQEGTNNGRDVRHENGDGWGFSTTYDLGMGFSAGAAY
                     TSSDRTNDQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYAVAN
                     KTQNFEVTAQYQFDFGLRPAVSFLMSKGRDLHAAGGADNPAGVDDKDLVKYADVGATY
                     YFNKNMSTYVDYKINLLDEDDSFYAANGISTDDIVALGLVYQF"
     gene            10578..11012
                     /gene="uspF"
                     /locus_tag="OO130_002622"
     CDS             10578..11012
                     /gene="uspF"
                     /locus_tag="OO130_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HCR5365976.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            11569..12483
                     /gene="sitA"
                     /locus_tag="OO130_002623"
     CDS             11569..12483
                     /gene="sitA"
                     /locus_tag="OO130_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HCR5365977.1"
                     /translation="MHSIKKVTMLLGCLALTCSIAFQASATEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
     gene            12483..13310
                     /gene="sitB"
                     /locus_tag="OO130_002624"
     CDS             12483..13310
                     /gene="sitB"
                     /locus_tag="OO130_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HCR5365978.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGYMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNGSEESIITDDERPFVAHRPSAVQREAR"
     gene            13307..14164
                     /gene="sitC"
                     /locus_tag="OO130_002625"
     CDS             13307..14164
                     /gene="sitC"
                     /locus_tag="OO130_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HCR5365979.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            14161..15018
                     /gene="sitD"
                     /locus_tag="OO130_002626"
     CDS             14161..15018
                     /gene="sitD"
                     /locus_tag="OO130_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HCR5365980.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLTWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAIKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINARTKGNA
                     HTQDLLSPN"
BASE COUNT         3846 a         3851 c         3704 g         3911 t
ORIGIN      
        1 tcgtacccat ttaccgttta tcccttgtac atattccccc ggtttggccc gagctacgag
       61 cttttgcccg gcgagtttcg cgatatcatc tacagacaca ttattttgct ttgccagttg
      121 ttggtaactc gctttacgtt cggcattaat gtcggttact aatttctctg tttcagcatc
      181 cgttttcagc gcaaccagat aaccataaaa tgtttccccg acccgcccct gcgttctggc
      241 ttcatccaga gtcaatgcca ttacattgct gcttaccagc ccaacaagaa acgcacaaag
      301 aagtaatgtt ttcttcatca tcgcctcagt aaagatcgct acgagtttca agcagctctt
      361 cgacatcttt gtctgccttg atgatgatct catgctcaat tttaacgttc atattgatag
      421 tgatcggttc cttgggcgca gcgacttcaa tacgcggcgt acaggcagcc agcataaaag
      481 atgacgtcaa cgcagccagt aaaattttca ttgtttttcc tcacactctt ttccttgcgg
      541 acagtcattt cccggcagac gtgcgttctg ctccaaccat gcctggagat tatcgccaaa
      601 gcgtaaactg cgccacagcg taaaaatatt ctcttcatga tggtaattaa ggtttaccgt
      661 accgctttta ccatcaacgc gactggtacc tgtaatgttg gcctgcatgg ttaataaacc
      721 gagattatct aaattaatat ttgtcgatga acggctaatt tccatatagc gcaaccagtt
      781 aattgctgaa cccgcagtca tattgtcttt caccaccgca tctgcggtgt ctttatccag
      841 ccgcaaggtc attggcccgc tatttgccag ccaaccatct ttcactatcc atttttcgtt
      901 gtttagccat aacggcaatg caccactaaa tgccccggac atggcgaatt gtttcggatt
      961 gacggcgcta accagttcgc tggatgagag attattcagc cgcaacagcg ccgggtcatg
     1021 ttgcggcata cgtaattgtt tcatcagtac gttaccgcct aacacatcga cgctaacatc
     1081 agtcaacagc aagggttctt cttcagtcca cggataacgc ccttgcaaat cagccgtaat
     1141 atttttcgct gtcaccagat taatcacttc ggcaattcgc aacgtaacgg ggccgcgagt
     1201 ccccagatgc caggctccat cggcaaaacg gaaaggcagg acaaaatcga caccgttaac
     1261 ctgattatct ggcatccagg cactaccgcc tttcaacacg ccgtgccctc ccgcgcggaa
     1321 tccttgttca ggcgcagcag aaaatgcaac ctgagcatac agttcaccat cgcgtaagtt
     1381 catcttccag tcgggtggca ccagcggctg gaatacagtc agtgattgtt ttggccacca
     1441 ggcgttaccg cgcagacgaa taccgtccca gcgaccattt acccgaaccg ggccaatctc
     1501 accagcatgt aaatcgcctt taaagaggaa atacgtcgga tcgcgcccat cgacgctaaa
     1561 ttttaaggtt gatggcggta acacactgcc gccagtgaac agcgtttgcc cggcgtccag
     1621 tgacagcgcg ccgctgaagg agggatgctg tgcgtcacgt acccagacga cgggcttgtc
     1681 cagaattaat cgcggctttt ctaccgtcat cgtaccgtat tgtaactggt cgaaaccggt
     1741 ggagaggtcg gtcagcgtaa tcgtgctgtc atgccattca ccttttcctg cgacatccca
     1801 ggtggcattc atcggtgtaa aactgccttt tccccagtag cgccactgcc agcggcccgc
     1861 gtcagggaga aaatcattcg ccagcccatc catatgcagc acgaaatcgc ccagttcatt
     1921 ttcatgcgcc tgcaaaatgg cctgcaaacg tccgtcaaca ccacgttggg tgacttttac
     1981 acccgctaaa ggccagcgga tttcatcgat gtccagcgaa tcgatgactc ttccctttga
     2041 acgaagtaac gcgcctggct caaaggccag cgttgggtca gtcagacttc cacttagctg
     2101 cgcgggtaag cgggcatata aaatgaggtc cgcctgtttc gcttcaccgg tcagctgcag
     2161 aggcagctgg ctgttatcca tgcttaattt tcctgggcca aaatttagca ccgcgttgcc
     2221 tttacccgct tgcccctggg tcagcacact cagtcgtccg ctgatcagag cgttctcaag
     2281 ccctgcctgc cagttgtcga ctttgacacc cagtcggcca ctcaaaggaa aacctgcata
     2341 cggccaactc cagcgaccat cgcttacggt caattgttga cgagtaattt gccacggcaa
     2401 atcgagcaac ggatcgccgt tatcacgtgc cagcacaatc aattgcccgc tattttcctg
     2461 ccagtccagc tctgcatcca ccagtgacgg ttcctgcggc aagtttaacg tcgcagtagc
     2521 atgcccactt acaggaagtc catccggcac gagcggcata gtaaattccc ccaccagttt
     2581 aaccggcggc tgatattcaa acgcgatgac atccagttcg ctgactgtaa gttgttgccc
     2641 tttcagctgg ccttgaaatt taactttttc gccctgataa cgcagttgct ggatatcaga
     2701 ggttaatgcg agagagagtt ttccctgcca ttcctgccag ggagaaaaaa tcagtttatc
     2761 gatattgatc caggtgttag gcagcatgga ctgccactgc gcgagggttt ttggagcagc
     2821 tggcgattgc tccgtctgcg gcaatttcgc cagacaagca gaatcaagtt ctaccgtgcc
     2881 gacgttcaat aaccagcggc tgggatgtga aaggctggcg ttggtgatat gcgcaagctg
     2941 acaatctccc accagataac ggagatcggg aatgattaaa cctttacgcg taatgcgtgg
     3001 gctttcatct aatgcaatac gtgtcccgag cggtagccag atgcccgcca gcgtgggaac
     3061 ccacagcccg agcgtcatca gcagcgtcaa cggcacaaga ataatcagta ataacagcgc
     3121 gagaacggct ttatatttac ccagcatggg tagttaatat cctgatttag cgaaaaatta
     3181 agcattcaat acgggtattg tggcatgttt aaccgttcag ttgaaggttg cgcctacact
     3241 aagcatagtt gttgatgaat ttttcaatat cgccatggtt ttcaattata tttgaaattt
     3301 tgtaaaatat ttttagtggc ttaaatgtga ttcaacatca ctggagaaag tcttatgaaa
     3361 ctcgccgttt atagcacaaa acagtacgac aagaagtacc tgcaacaggt gaacgagtcc
     3421 tttggctttg agctggaatt ttttgacttt ctgctgacgg aaaaaaccgc taaaactgcc
     3481 aatggctgcg aagcggtatg tattttcgta aacgatgacg gcagccgccc ggtgctggaa
     3541 gagctgaaaa agcacggcgt taaatatatc gccctgcgct gtgccggttt caataacgtc
     3601 gaccttgatg cggcaaaaga actggggctg aaagtagtcc gtgttccagc ctatgatcca
     3661 gaggccgttg ctgaacacgc catcggtatg atgatgacgc tgaaccgccg tattcaccgc
     3721 gcgtatcagc gtacccgtga cgctaacttc tctctggaag gtctgaccgg ctttactatg
     3781 tatggcaaaa cggcaggcgt tatcggtacc ggtaaaatcg gtgtggcgat gctgcgtatt
     3841 ctgaaaggtt ttggtatgcg tctgctggcg ttcgatccgt atccaagtgc agcggcgctg
     3901 gaactcggtg tggagtatgt cgatctgcca accctgttct ctgaatcaga cgttatctct
     3961 ctgcactgcc cgctgacacc ggcaaactac catctgttga acgaagccgc cttcgatcag
     4021 atgaaaaatg gcgtgatgat cgtcaatacc agtcgcggtg cattgattga ttctcaggca
     4081 gcaattgaag cgctgaaaaa tcagaaaatt ggttcgttgg gtatggacgt gtatgagaac
     4141 gaacgcgatc tgttctttga agataaatcc aacgacgtga tccaggatga cgtattccgt
     4201 cgcctgtctg cctgccacaa cgtgctgttt accgggcacc aggcattcct gacagcagaa
     4261 gctctgacca gtatttctca gactacgctg caaaacttaa gcaatctgga aaaaggcgaa
     4321 acctgcccga acgaactggt ttaatgttgc agctcccctg caatccaggg gagctgattc
     4381 agataatccc caatgacctt tcattctcta ttcttaaaat agtcctgagt cagaaactgt
     4441 aattgagaac cacaatgaag aaagtagccg cgtttgttgc gctaagcctg ctgatggcgg
     4501 gatgtgtaag taatgacaaa attgctgtta cgccagaaca gctacagcat catcgctttg
     4561 tgctggaaag cgtaaacggt aagcccgtga ccagcgataa aaatccgcca gaaatcagtt
     4621 ttggtgaaaa aatgatgatt tccggcagca tgtgtaaccg ctttagcggt gaaggcaaac
     4681 tgtctaatgg tgaactgaca gccaaagggc tggcaatgac ccgtatgatg tgcgctaacc
     4741 cgcagcttaa tgaactcgat aacaccatta gcgaaatgct gaaagaaggt gcacaagtgg
     4801 atctgaccgc gaaccagtta acgctggcga ccgcgaaaca gacattaact tataagctgg
     4861 cggatttaat gaattaatag ctgccacagc tcccggcggc aagtgactgt tcgctacagc
     4921 gtttgccgtt gggtaatgca cacatcccaa tcgccgtgcc atccagttga cgggcaacag
     4981 aaagcgaacc gccgatcatt gcacaatttg cttctccact actggacatc gacgctttta
     5041 aacctggcgc tacgtgcgcc gcagtcgcct gctgaacagg ttcactacta cacgccgaca
     5101 acaataaagc ggcacaccct acccaaaacg ctgctcgcat cttttcttcc tctgatcttc
     5161 aagccaaacg acaccgccat aaataatagg cagcacagag ggcgacatcg agagctgtcc
     5221 tgcgcgttgc cccgcatttt tactttttta tggctatttt tttgccctct gtttgatcaa
     5281 aacattcatt acgctgatgt gggggacaca aaagcgaaaa tgcagaagaa agccatttgc
     5341 taaaattgaa agattactac tgggcgcgca gcaatttcgt gcgcccctca tttgcgcaat
     5401 gtaagggtgt catatgatta ctattgacgg taatggcgcg gttgcttcgg tggcgtttcg
     5461 caccagtgaa gttatcgcca tctaccctat tacccccagt tccacgatgg cagaacaggc
     5521 tgatgcctgg gccggaaacg gcttgaagaa cgtttgggga gacacaccac gcgtggttga
     5581 aatgcagtcg gaagcgggtg ctatcgctac cgtgcatggc gctttgcaga cgggtgccct
     5641 ttcaacatcg tttacgtcat cgcagggttt gctgctgatg atcccgacac tgtacaaact
     5701 ggcaggcgaa ctcacaccgt ttgtcctgca tgtagcggca cgtaccgttg ccacacatgc
     5761 actctctatt tttggcgatc attccgacgt catggcggtg cgccagacgg gttgcgcgat
     5821 gttgtgtgcg gcaaacgtcc aggaagcgca agactttgct ctcatttcgc acatcgcgac
     5881 gctgaaaagc cgcgtgccat ttattcattt ctttgatggt ttccgcacgt cccacgaaat
     5941 caataaaatt gtcccgctgg ccgatgacac gattcttgat ctcatgccgc aggccgaaat
     6001 tgatgctcat cgcgcccggg cgctcaaccc ggaacatccg gtgatccgtg gtacgtccgc
     6061 caatcctgac tcttatttcc agtctcgcga agccaccaac ccctggtaca acgcagtcta
     6121 tgaccatgtt gaacaggcga tgaatgattt ctctgccgcg acaggtcgtc agtatcagcc
     6181 gtttgaatat tacgggcatc cgcaagctga acgggtgatt atcctgatgg gctcagccat
     6241 tggcacctgt gaagaagtgg ttgatgaatt gctaacccgt ggcgaaaaag tcggcgtgct
     6301 gaaagttcgc ctgtaccgcc ccttctcagc caaacacttg ctacaaactc taccgggatc
     6361 cgtacgcagc gtggcggtac tggacagaac caaagaaccc ggtgcccagg cagaaccgct
     6421 ctatctggat gtgatgaccg cactggcaga agcctttaat aatggcaagc gcgaaactct
     6481 gccccgtgtc attggtgggc gctatggtct ttcatccaaa gaatttggcc cggactgtgt
     6541 actggcggta tttgccgagc tcaacgcggc taaaccgaaa gcgcgcttta cggttggtat
     6601 ttacgatgat gtaaccaatc tgtccctgcc gttgccggaa aacacccagc caaactcggc
     6661 gaaacttgaa gccttgtttt atggccttgg tagtgatggc agcgtttccg cgaccaaaaa
     6721 caatatcaag attatcggta attccacgcc gtggtacgca cagggctatt ttgtttacga
     6781 ctccaaaaaa gcgggcggcc tgacggtttc tcaccttcgc gtgagcgaac agccgattcg
     6841 ttccgcttat ctcatttccc aggctgattt tgttggctgc caccagttgc agtttatcga
     6901 taaatatcag atggctgagc gtttaaaacc tggcggcatt ttcctgctca acacgccgta
     6961 cagcgcagat gaagtgtggt cgcgcttgcc gcaagaagtt caggccgtgt taaaccagaa
     7021 aaaagcgcgc ttctatgtga ttaacgcggc gaaaatcgcc cgcgaatgtg gcctggcggc
     7081 ccgtattaat accgtcatgc agatggcttt tttccatctg acgcaaattc tgcctggcga
     7141 tagcgccctc gcagaattgc agggtgcgat tgccaaaagt tacagtagca aaggccagga
     7201 tctggtggaa ggcaactggc aggctctggc gctggcgcgt gaatccgtag aagaagttcc
     7261 gttgcaaccg gtaaatccgc acagcgccaa tcgaccgcca gtggtttccg atgccgcccc
     7321 tgatttcgtg aaaaccgtaa ccgcagcgat gctcgcaggg cttggtgatg ccctccccgt
     7381 ttcggcgctg ccgccagacg gcacctggcc gatggggacc actcgctggg aaaaacgcaa
     7441 tatcgccgaa gagatcccca tctggaaaga ggaactctgt acccaatgta accactgcgt
     7501 cgccgcttgc ccacactcag ctattcgcgc aaaagtggta ccacccgaag cgatggaaaa
     7561 cgcccctgcc agcctgcatt cgctggatgt gaaatcgcgt gatatgcgcg ggcagaaata
     7621 tgtcttgcag gtggctccgg aagattgcac cggttgtaac ctgtgcgtcg aagtttgccc
     7681 ggcgaaagac cgtcagaatc cagagattaa agccatcaat atgatgtcgc gcctggaaca
     7741 tgtcgaagaa gagaaaatca attacgattt cttcctcaac ctgccagaaa tcgaccgtag
     7801 caaactggaa cgtattgata ttcgtacatc gcagctgatt acaccgctgt ttgaatattc
     7861 cggtgcttgc tccggttgtg gcgagacgcc gtatattaaa ttgctgactc agctctatgg
     7921 cgaccggatg ttgatcgcta acgccaccgg ctgttcttca atttatggcg gtaacctgcc
     7981 ctctacaccg tataccaccg atgccaacgg tcgtgggccg gcatgggcga actctctatt
     8041 tgaagataat gccgaatttg gccttggttt ccgcctgacg gtcgatcaac accgtgtccg
     8101 cgtgctgcgt ctgctggatc aatttgccga taaaatcccg gcggaattac tgacggcgtt
     8161 gaaatcagac gctacgccag aggttcgtcg agaacaggtc gcagctttac gccagcaact
     8221 caacgatgtt gccgaagcac atgaactgct acgtgatgca gatgcactgg tggaaaaatc
     8281 aatctggctg attggtggtg atggctgggc ttacgatatc ggctttggcg gtctggatca
     8341 tgtattgagt ttgacggaaa acgtcaacat tctggtgctg gatacgcaat gctattccaa
     8401 caccggtggt caggcgtcga aagcgacacc gctgggtgca gtaactaaat ttggcgagca
     8461 cggcaaacgt aaagcgcgta aagatcttgg cgtcagtatg atgatgtacg gtcatgttta
     8521 tgtggcgcag atttctctcg gcgcgcagct gaatcagacg gtgaaagcga ttcaggaagc
     8581 ggaagcgtat ccgggaccat cgctgatcat tgcttatagc ccgtgtgaag agcatggtta
     8641 cgatctggca ctcagccatg accagatgcg ccaactcaca gctaccggct tctggccgct
     8701 atatcgcttc gatcctcgtc gtgccgatga aggcaaactg ccgctggcgc tggattcacg
     8761 cccgccgtca gaagcactgg aagaaacgtt acttcacgag caacgtttcc gtcgtctgaa
     8821 ttcgcagcag ccagaagtgg cagaacagtt atggaaagat gctgcagctg atttgcaaaa
     8881 acgctatgac ttcctggcac aaatggccgg aaaagcggaa aaaagcaaca ccgattaaat
     8941 gctctggata aggattatcc aattctaaaa aaaagcccgg acgactgttc gggcttgtct
     9001 ttttatatgt tggaaaatca gtggcaatgc aatggcccaa cagaaaataa aatctgtgac
     9061 aataaaggca tataacccgc gcagaataac gtatagaaaa ttaaaagtat aatttttatt
     9121 ttgtatgtat tatcttcgat acattcacca gacttattat catttcagaa gaattatttg
     9181 attaaggttt tacttaaggc gtaacaaatg atatttatcg gctaactgaa cttctccttt
     9241 aggatgtttt cactccccgg aatggggata aaaggcaaat aaaataacta aaggatttat
     9301 tcaatgaaaa gcaaagtact ggcactttta attcctgccc tgctcgccgc aggtgctgca
     9361 catgcagccg aagtttataa taaagacggc aacaaattag atctgtatgg caaagttgat
     9421 ggcctgcatt atttttctga taattcagcg aaagatggcg atcagagcta tgcgcgtctg
     9481 ggttttaaag gcgaaaccca aattaacgat caactcactg gctacggtca atgggaatac
     9541 aatattcaag caaacaacac tgaatcttca aaaaaccagt catggacccg tctggcattt
     9601 gccgggctga aatttgcaga ttacggttct ttcgattacg gacgtaatta tggcgtaatg
     9661 tacgacatcg aaggctggac cgatatgctg cctgaatttg gcggtgactc ttataccaat
     9721 gcagacaact ttatgactgg tcgagccaat ggcgtcgcga cttatcgtaa tactgatttc
     9781 ttcggtctgg taaatggtct gaacttcgcg gtgcagtatc aaggtaacaa cgaaggagcc
     9841 agtaatggtc aggaaggcac caacaacgga cgtgatgttc gccatgaaaa cggtgacggc
     9901 tggggttttt ccacaacata tgatttaggc atgggcttta gcgctggtgc ggcatacacc
     9961 tcttctgacc gcaccaatga ccaggttaac catactgcgg cgggtggtga taaagcagac
    10021 gcgtggactg ctgggctaaa atacgatgct aacaatattt acctagcaac catgtattca
    10081 gaaacgcgta atatgacccc gtttggcgac agcgattatg ctgtggcaaa caaaacccag
    10141 aattttgaag tcactgccca gtaccagttt gattttggtc tgcgtccggc agtctctttc
    10201 ctgatgtcta aaggccgtga cctgcacgct gccggtggtg cagacaaccc ggcaggtgtt
    10261 gatgataaag atctggttaa atacgccgat gttggcgcga cttactattt caataaaaac
    10321 atgtccacct acgttgacta taaaatcaac ctgttggatg aagatgacag cttctacgct
    10381 gccaatggca tctctaccga tgatattgtc gctttaggtc tggtttatca gttctaaatc
    10441 ctcctgcccg ctgttatggc gggctttttc tgcttattct tcctcctttg atctaaatta
    10501 aaaatgtgaa ctccatcatt acacaaaaag tgtcatctgg cgttacactt tatgcggata
    10561 ctaaaacagg aggttttatg aacagaacga ttcttgtccc tatcgatatt tccgattcag
    10621 aattaactca acgcgtgatt agccacgttg aggcagaggc aaagattgac gatgcagagg
    10681 ttcatttcct gacggtaata ccttcactgc cctactatgc ctctctgggt ttagcgtatt
    10741 ccgcagaatt accggcaatg gatgacctga aagcggaagc caaatcgcaa ctggaagaga
    10801 tcattaaaaa atttaaactg ccaaccgaca gagtgcatgt ccatgttgag gaaggctcgc
    10861 ccaaagaccg cattctggaa ttggcgaaga agatccccgc tcatatgatc atcattgctt
    10921 cccatcgacc ggatatcacc acttatctgc tcggttccaa cgccgcagct gtagtgcgtc
    10981 acgcagagtg ctccgtgctg gttgtgcgct gacactaacg cccgcacatt gctgcgggct
    11041 ttttgattca tttcgcaaat gtgctgacat tttcccctct aatccgtacc atacacgcca
    11101 ctgtttttat atcagatttc ttatgctggg cattccggca tgacgcatac tcttctgatg
    11161 ccatataacg aattgagtcg cttttaaatg tcgcaaaatc aagaaattag taagaaagaa
    11221 caatacaacc tgaacaagta agggcaaaaa tcacaactat ctgactcata agtattttat
    11281 ttatttttca atatgttaaa cattatcagc gacaacaata agctacgata acccactctt
    11341 tttttgcccc atttttgcca tcaacaattt tcctcataaa gtcatttcaa agcctaatag
    11401 ctgctctact tttttcctca atatcatgtc gccagcagga aacaacctaa tccctgtttt
    11461 aggcctcaaa gctgttaatg ataattattt tcattttgta taggttatgc tataaaacat
    11521 agccgttgct atgtttgttc taattttgct cactataggt actaaattat gcactcaata
    11581 aaaaaagtaa ccatgctctt ggggtgcctc gccctcacct gctcgatcgc atttcaggca
    11641 agtgcaactg aaaaattcaa ggtcattaca acatttacca tcatcgcgga tatggccaaa
    11701 aacgtggctg gagatgctgc agaagtctca tccataacca agcctggtgc agaaattcat
    11761 gagtatcagc ctacccctgg cgatattaaa cgtgcacagg gggcacaact gattctcgcc
    11821 aatggtatga atctggaatt gtggttccaa cgcttttacc agcatctcaa tggggttcca
    11881 gaagtaattg tctcttcggg tgtgacgcca gtagggatca ccgaaggacc ctatgagggc
    11941 aaacctaatc cccatgcatg gatgtcgcca gataatgctc tgatttacgt cgataatatt
    12001 cgtgatgcgt tgataaaata cgatccggca aatgcacaaa cctaccaacg taatgccgat
    12061 acttataaag ccaagattac ccaaaccctt gcccccctgc gtaagcagat tacggaactc
    12121 cctgagaatc agcgatggat ggtcaccagt gaaggggctt tttcttatct cgcacgcgat
    12181 ttggggctaa aagagcttta tctgtggccg attaatgccg atcaacaagg aacaccgcag
    12241 caggtacgta aggttgttga tatagttaag aaaaatcata tcccggcagt ctttagcgag
    12301 agtacgattt ccgataaacc agcgcgtcag gttgcgcgtg aaaccggcgc acactacggc
    12361 ggtgtgctct atgtcgattc cctgagcaca gaaaacggcc cggtacctac gtatatcgac
    12421 cttcttaagg ttactaccag tacgctggta cagggaatta aagccgggaa aagggagaaa
    12481 taatgatgca atctgcaggc attgtcgtta atgatgttac tgtcacctgg cgtaacgggc
    12541 acacagcact gcgcgatgca tccttcacag taccaagtgg atctattgcc gctctggttg
    12601 gggtaaacgg ttccggaaaa tcgacgttgt ttaaagcgat tatgggattt gtgcgtctga
    12661 ccagcggaaa gatatctgtt ctgggtattc ccacgcgaca ggcgctacag aaaaacctgg
    12721 ttgcctacgt ccctcagtca gaagaggttg actggtcatt tcctgtactg gtggaagatg
    12781 tggtgatgat gggccgctat gggtatatgg gtatgttgcg tatagccaaa aagagggatc
    12841 gtcagattgt caccgatgcg ctggaacgtg tcgatatggt ggattttcgc catcgtcaaa
    12901 tcggcgagct ttccggtgga caaaagaaac gcgtctttct ggcgagagcg attgcacagc
    12961 agggtgatgt gatcctgctg gatgaacctt ttaccggcgt tgatgtgaaa accgaagcga
    13021 aaatcatcag cctgttgagg gagttacgcg cggaaggcaa aacgatgctt gtctcaacgc
    13081 acaatctcgg ctcagtcacg acattttgcg attatacggt catggtcaaa ggcaccgtcc
    13141 tggcaagcgg gccgacagac accactttta cagccgagaa cctggagctg gcttttagcg
    13201 gcgtactgcg ccatgtcaca cttaacggct cggaagaaag cattattact gacgatgaac
    13261 gcccttttgt ggcacatcga ccgtcagcgg tgcagaggga agcaagatga acgtgctact
    13321 ggaacccttc agctatgaat atatgctcaa tgcgatgtgg gtctcggcaa tggtcggcgg
    13381 tctctgcgct ttcctgtcat gctatctgat gctaaaaggc tggtcgctga ttggcgatgc
    13441 gctgtcgcac tctattgtcc cgggtgttgc gggggcatat atgctggggc ttccgttttc
    13501 tctgggagcc ttcttttccg gcggactggc agcgggcagc atgctgtttc ttaaccaacg
    13561 cacccgctta aaagaagatg ccatcatcgg cctgatcttc tcgtcctttt ttggcctggg
    13621 gctgtttatg gtgtcattga acccgacatc cgtgaacatt cagaccatcg tcctgggcaa
    13681 tattctggca atcgatccgg cggatatcct gcaactcact atcattggca ttctctcgat
    13741 catcgtgctt tttttcaaat ggaaagatct tatggtgacc tttttcgacg agaatcacgc
    13801 ccgtgctatt ggattacatc cgggcagatt aaaaatcctc ttcttcaccc tgctgtcggt
    13861 ttcgaccgtt gcggcgctgc aaacggttgg tgcctttctg gtgatctgtc tggtggtgac
    13921 tcccggtgca acagcatggt tgttaaccga tcgttttccc cgcctgctca taattgctgt
    13981 aactattggc agtgtaacca gtttcctcgg cgcgtgggtc agttactttc tggacggcgc
    14041 gacaggtgga atcatcgtgg tggcgcagac actgctgttt cttttagcat ttgtatttgc
    14101 gcccacccat ggacttttgg caaatcgtcg gcgtgcgcat aaagcgctgg aggatcgctc
    14161 atgatggcat tattactgga gccactgcag ttcaccttta tgagtcacgc actgctgatt
    14221 tcactggtag tttccatacc ctgtgcgctg ctgtcggtct ttctggtcct gaaaggttgg
    14281 gcactgatgg gtgacgcgat gagtcatgcg gtttttcctg gcatcgtgct tacctggatt
    14341 ctggggttac ctctggctac aggggcattc gtcgccggag tattctgcgc ggtggcgacg
    14401 ggatacctga aagacaacag ccgaatcaag caagatacag ttatggggat tgttttttct
    14461 ggcatgtttg ccgcaggtct gatcttgtat attgcaatca aaccagacgt acatcttgac
    14521 catattcttt tcggcgacat gctcgggata accatcggcg atataatcca gacggtgatt
    14581 attgccgggc tggttacact tgttattagc gtaaaatggc gggatttttt gctgttcagc
    14641 tttgattatc aacaggcgca ggtaagcggt ttgcatacga gatggctgca ttatgggctg
    14701 ctgtgcatgg tttccctgac cattgtggcg acgctgaaag cagtaggtat cattctctct
    14761 atttctctgc tcattgcgcc tggcgccatt gcagtactcc tgacacaacg ctttcatatt
    14821 gcgctcctac tggcgactgg catatcggta atagtgtcaa tgactggtgt ctggctgtcc
    14881 ttttttatcg acagcgcacc agctccgacg attgtcgtct tattcgcggt cgtgttcatc
    14941 atgacgttta ccgttaccag catcaacgca cgcacaaagg gaaacgccca tacacaggat
    15001 ctgttatcac ccaattagca caatatccga ggacagcatc gtccgccagc gtccgccagc
    15061 ctacagaagt tcggaaagcc atggaaggtc gttgggagga ggtacgaatt ctatcatgca
    15121 aaaaatacgt aaaatcgata acgcctagaa atcattcaat actcgcacta tcgaaagttt
    15181 accagccaac cgcggcacgt tcctgcatac gacgtgccgc agcttctgtt attccacgat
    15241 aaaattccta accgaattca aatccttcag actattcagc tcttccttca gctcccgctg
    15301 acggcgataa at
//
LOCUS       DAJTMC010000095        15033 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.095, whole genome shotgun sequence.
ACCESSION   DAJTMC010000095 DAJTMC010000000
VERSION     DAJTMC010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15033
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.095"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            <1..453
                     /locus_tag="OO130_002627"
     CDS             <1..453
                     /locus_tag="OO130_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 family protein"
                     /protein_id="HCR5365981.1"
                     /translation="LQEVNAALFKEYLPLLQQSEPTIKQPVRWKNALGELNANLDISI
                     ADPAKSSSSTNKDIKSLNFDVKLPLNVVTETAKQLNLSEGMDAEKAQKRADKQISGMM
                     TLGQMFQLITIDNNTASLQLRYTPGKVVFNGQEMSEEEFMSRAGRFVH"
     gene            complement(494..1426)
                     /locus_tag="OO130_002628"
     CDS             complement(494..1426)
                     /locus_tag="OO130_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCR5365982.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQDK"
     gene            1790..4576
                     /gene="polA"
                     /locus_tag="OO130_002629"
     CDS             1790..4576
                     /gene="polA"
                     /locus_tag="OO130_002629"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HCR5365983.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNPLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            complement(4958..5590)
                     /locus_tag="OO130_002630"
     CDS             complement(4958..5590)
                     /locus_tag="OO130_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HCR5365984.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRHSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            6172..6681
                     /gene="yihI"
                     /locus_tag="OO130_002631"
     CDS             6172..6681
                     /gene="yihI"
                     /locus_tag="OO130_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HCR5365985.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            6870..8243
                     /gene="hemN"
                     /locus_tag="OO130_002632"
     CDS             6870..8243
                     /gene="hemN"
                     /locus_tag="OO130_002632"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HCR5365986.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(8433..8543)
                     /locus_tag="OO130_002633"
     CDS             complement(8433..8543)
                     /locus_tag="OO130_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HCR5365987.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(8654..10063)
                     /gene="glnG"
                     /locus_tag="OO130_002634"
     CDS             complement(8654..10063)
                     /gene="glnG"
                     /locus_tag="OO130_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HCR5365988.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            complement(10075..11124)
                     /gene="glnL"
                     /locus_tag="OO130_002635"
     CDS             complement(10075..11124)
                     /gene="glnL"
                     /locus_tag="OO130_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HCR5365989.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLEIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            complement(11298..12707)
                     /gene="glnA"
                     /locus_tag="OO130_002636"
     CDS             complement(11298..12707)
                     /gene="glnA"
                     /locus_tag="OO130_002636"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HCR5365990.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            13080..14903
                     /gene="typA"
                     /locus_tag="OO130_002637"
     CDS             13080..14903
                     /gene="typA"
                     /locus_tag="OO130_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000570679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HCR5365991.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDY"
BASE COUNT         3879 a         3771 c         3878 g         3505 t
ORIGIN      
        1 ctgcaagaag tgaatgctgc attgttcaaa gagtatttac cgttattaca acaaagtgag
       61 ccgaccatta aacaaccggt aagatggaag aacgcactcg gcgaactaaa tgccaatctg
      121 gatatcagta ttgccgaccc agccaaatct tcatcatcca caaacaaaga tatcaaatcg
      181 ctcaattttg atgtgaagtt accgcttaat gtcgtcacag aaaccgcaaa acagcttaat
      241 ttatctgaag gaatggatgc ggaaaaagcg caaaagcggg ctgataaaca aatcagcggg
      301 atgatgacct taggtcagat gtttcagtta atcacgattg acaacaatac cgcctcgctg
      361 caactgcgtt atacaccggg taaagttgtt tttaacggac aggagatgag cgaagaagaa
      421 tttatgtctc gtgccggacg ttttgttcat taaaatactc actcgcctct gctcattcag
      481 aggcgagcaa aatttacttg tcctgccgct gtgatgacat caacgacgtc agaagtcggt
      541 ctttttcctg ccacagtgag ttcagccatt gctgaaaatg gcgcttaaag cttttgtcat
      601 tgatgtagtc gccatgtaac tcatcggcaa taggctgtaa atccacatga acgacaattc
      661 gcgtcagttt accgcttaac atatcgaaga acggctgacg gttattgtcc ggataacaca
      721 gcgtaacatt aagcagttta tcgaattgtt tacccagtac atttagcgcc atcgcaatgc
      781 ctgccgcctt tggtggcaac aagttttgaa aagtggagtg ggtttgctga tgtttttctt
      841 gcgtgaaacg ggagccttca acgaaattga caatggtggt gggatgcagg cgaaactttt
      901 cacaagaacg ccgagtggtt tcaacatctt taccacgtcg ctccggatga cgtaataaat
      961 aagcgcggga ataacgcttc ataaacggca tatccagcgc ccagcacgcc aggccaagaa
     1021 atggcaccca ggccagctgt tgcttgagga aatatttatt catcggaatg tgcttacgaa
     1081 acagcacgca cagtacgaca atatctgccc aactacggtg gttacaaata agcagatacc
     1141 agttcttctt acttaaccct tccagcccgt gaacttccca ttgcaggtgt gggttcagat
     1201 gcagtaatac cgccagacct tcacaccagc aatacatcat aaaatcacaa aaacgcgaaa
     1261 cctttcgcca aatgactggc acaggtagca atagttttac aatcccggca atgatgatcg
     1321 ggacggaaca aaaaatagtg accaaaatgg tcaatacgat actcagcaaa agggttatcg
     1381 cagcgagtat tctcgtcata atgaatttat tcaaaaggtt agccatagac agtgcgctaa
     1441 agaagcgcaa ggcgcagatt ttagcagaaa acgaccgaaa taacggatga tccttaagga
     1501 gaaaaataat tcatatctat ccacattaga aaaaatccca ttatctcaat tattagggat
     1561 ggctttattt ttaactacat gaaaaacaaa gacaaacatc atactgtaaa aagcatgata
     1621 ataaattaaa agcgatgtaa ataatttatg cacaaagtta tccacatgac gatttgcgag
     1681 cgatccagaa gatctacaaa agattttcac gaaaagcggt gaaaaactca tgtttttatc
     1741 ctgtctgtgg catcctttac ccataatctg ataaacaggc acggacatta tggttcagat
     1801 cccccaaaat ccacttatcc ttgtagatgg ttcatcttat ctttatcgcg catatcacgc
     1861 gtttcccccg ctgactaaca gcgcaggcga gccgaccggt gcgatgtatg gtgtcctcaa
     1921 catgctgcgc agtctgatca tgcaatataa accgacgcat gcagcggtgg tctttgacgc
     1981 caagggaaaa acctttcgtg atgaactgtt tgaacattac aaatcacatc gcccgccaat
     2041 gccggacgat ctgcgagcac aaatcgaacc tctgcacgcg atggttaaag cgatgggact
     2101 gccgctgctg gcggtttctg gcgtagaagc ggacgacgtt atcggtactc tggcgcgcga
     2161 agccgaaaaa gccgggcgtc cggtgctgat cagcactggc gataaagata tggcgcagct
     2221 ggtgactcca aatattacgc ttatcaatac catgacgaat accatcctcg gaccggaaga
     2281 ggtggtgaat aagtacggcg tgccgccaga actgatcatc gatttcctgg cgctgatggg
     2341 tgactcctcc gataacatcc ctggcgtacc gggcgtcggt gaaaaaaccg cgcaggcatt
     2401 gctgcaaggt ctgggcgggc tggatacgct gtatgccgag ccagaaaaaa ttgctgggtt
     2461 gagcttccgt ggcgcgaaaa caatggcagc gaagctcgag caaaacaaag aagttgctta
     2521 tctctcatac cagctggcga cgattaaaac cgacgttgaa ctggagctga cctgtgaaca
     2581 actggaagtg cagcaaccgg cagcggaaga gttgttgggg ctgttcaaaa agtatgagtt
     2641 caaacgctgg actgctgatg tcgaagcggg caaatggtta caggccaaag gggcaaaacc
     2701 agccgcgaag ccgcaggaaa ccagtgttgc agacgaagcg ccagaagtga cggcaacggt
     2761 gatttcttat gacaactacg tcaccatcct tgatgaagaa acactgaaag cgtggattgc
     2821 gaagctggaa aaagcgccgg tatttgcatt tgataccgaa accgacagcc ttgataacat
     2881 ctctgctaac ctggtcgggc tttcttttgc tatcgagcca ggcgtagcgg catatattcc
     2941 ggttgctcat gattatcttg atgcgcccga tcaaatctct cgcgagcgtg cactcgagtt
     3001 gctaaaaccg ctgctggaag atgaaaaggc gctgaaggtc gggcaaaacc tgaaatacga
     3061 tcgcggtatt ctggcgaatt acggcattga gctgcgtggg attgcgtttg ataccatgct
     3121 ggagtcctac attctcaata gcgttgccgg gcgtcacgat atggacagcc tcgcggaacg
     3181 ttggttgaag cacaaaacca tcacttttga agagattgcg ggtaaaggca aaaatccact
     3241 gacctttaac cagattgccc tcgaagaggc cggacgttac gccgccgaag atgctgatgt
     3301 caccttgcag ttgcatctga aaatgtggcc ggacctgcaa aaacacaaag ggccgttgaa
     3361 cgtcttcgag aatatcgaaa tgccgctggt gccggtgctt tcacgcattg aacgtaacgg
     3421 tgtgaagatc gatccgaaag tgctgcacaa tcattctgaa gagctcacct tgcgtctggc
     3481 tgagctggaa aagaaagcgc atgaaattgc aggtgaagaa tttaaccttt cttccaccaa
     3541 gcagttgcaa accattctgt ttgaaaaaca gggtattaaa ccgctgaaga aaacgccggg
     3601 tggcgcgccg tcaacgtcgg aagaggtact ggaagaactg gcgctggact atccgttgcc
     3661 aaaagtgatt ctggagtatc gtggtctggc gaagctgaaa tcgacctaca ccgacaagct
     3721 gccgctgatg atcaacccga aaaccgggcg tgtgcatacc tcttatcacc aggcagtaac
     3781 tgcaacggga cgtttatcgt caaccgatcc taacctgcaa aacattccgg tgcgtaacga
     3841 agaaggtcgt cgtatccgcc aggcgtttat tgcgccagag gattatgtga ttgtctcagc
     3901 ggactactcg cagattgaac tgcgcattat ggcgcatctt tcgcgtgaca aaggcttgct
     3961 gaccgcattc gcggaaggaa aagatatcca ccgggcaacg gcggcagaag tgtttggttt
     4021 gccactggaa acagtcacca gcgagcaacg ccgtagcgcg aaagcgatca actttggtct
     4081 gatttatggc atgagtgctt tcggtctggc gcggcaattg aacattccac gtaaagaagc
     4141 gcagaagtac atggaccttt acttcgaacg ctaccctggc gtgctggagt atatggaacg
     4201 cacccgtgct caggcgaaag agcagggcta cgttgaaacg ctggacggac gccgtctgta
     4261 tctgccggat atcaaatcca gcaatggtgc tcgtcgtgca gcggctgaac gtgcagccat
     4321 taacgcgcca atgcagggaa ccgccgccga cattatcaaa cgggcgatga ttgccgttga
     4381 tgcgtggtta caggctgagc aaccgcgtgt acgtatgatc atgcaggtac acgatgaact
     4441 ggtatttgaa gttcataaag atgatgttga tgccgtcgcg aagcagattc atcaactgat
     4501 ggaaaactgt acccgtctgg atgtgccgtt gctggtggaa gtggggagtg gcgaaaactg
     4561 ggatcaggcg cactaagatt cgcctgaaca tgcccttttt tcgtaagtaa gcaacataag
     4621 ctgtcacgtt ttgtgatggc tattagaaat tcctatgcaa caactgaaaa aaaattacaa
     4681 aaagtgcttt ctgaactgaa caaaaaagag taaagttagt cgcgtagggt acagaggtaa
     4741 gatgttctat ctttcagacc ttttacttca cgtaatcgga tttggctgaa tattttagcc
     4801 gccccagtca gtaatgactg gggcgttttt tattgggcga aagaaaagat ccgtaatgcc
     4861 tgatgcgcta tgtttatcag gccaacggta gaattgtaat ctattgaatt tacgggccgg
     4921 atacgccaca tccggcacaa gcattaaggc aagaaaatta ttcgccgtcc tgcgtttctt
     4981 ctacaggctg catctcgcta aaccaggtat ccagtttctg ccgcagcttg tccacgcctt
     5041 gtttcttcaa cgaagaaaac gtttcaacct gcacatcacc gttaaacgcc agtacagctt
     5101 cacgcaccat attcaattgc gctttacgtg cgccgcttgc cagtttgtcc gctttggtca
     5161 gcagcaccag aacggcgata ttgctgtcta ccgcccactc aatcatctgc tgatccaaat
     5221 ctttcagcgg atggcgaata tccattagca ccaccagacc ttgcaggcta tgacgttttt
     5281 cgaggtattc gccgagcgca cgctgccatt tgcgcttcat ctcttccggg acttccgcat
     5341 aaccgtaccc aggcaagtca accagacgct tgccgtcagc cacttcaaac aggttgataa
     5401 gctgggtgcg ccctggggtt tttgaggtac gagccaggct tttctggtta gtcagcgtgt
     5461 tcagcgcgct ggatttacct gcgttggaac ggcctgcaaa agccacttca attccggtat
     5521 cggaaggtag gtggcgaata tcaggcgcac tcatcacaaa atgcgtctgt tgataattca
     5581 aattagtcaa agcggtcgtc tccgtcagtc aaagctttgt ggcgattata cctgaaccat
     5641 aataaaaggc ggatttttcg gcgtgagcgt tgtaagtaaa aaccatacgc tttgtgagac
     5701 attgccgata gtctttatgc gaaatagcag agaaaattct gcgatgcatg tcaaataagc
     5761 tatataaatc agtgaattgc ttttatataa aactctgaaa atgtgcaaat actgatggcg
     5821 gttgattgtt tgtttaaagc gaaggcgtaa agtagcaccc atagagcgag gacgctaaca
     5881 ggaacaatga ctcaggatga gggtcaggag cgccaggagg cgaagacaga ggattgtcag
     5941 gaagacaaac gtccggagac gtaattaaac ggaaatggaa tcaacacgga ttgttccggc
     6001 taaaggaaaa acagggtgtg ttggcggcct gcaaggattg taagacccgt taagggttat
     6061 gagtcaggaa aaaaggcgac agagtaatct gtcgcctttt ttctttgctt gctttctgtt
     6121 agattccgcc gcaaatcgat actgaataaa acggctaaaa gacgaaccat tatgaaacca
     6181 tcatcttcaa actcacgcag caaaggtcat gcaaaagcgc gtcgaaaaac acgcgaggag
     6241 ctggatcagg aagctcgtga tcgcaagcgt cagaaaaaac gtcgtggtca tgcgccgggc
     6301 agccgcgcag cgggcggaaa caccacgtca ggcagcaaag gccagaacgc accaaaagat
     6361 ccacgtattg gcagtaaaac ccctattcca ttgggcgtga ctgaaaaagt caccaaacag
     6421 cacaaaccga agagtgagaa acctatgctt tcaccgcagg cggagttgga attactggaa
     6481 acggatgagc gtctggatgc gctgctggaa cgtctggaag caggcgaaac cctgagtgcc
     6541 gaagagcaat cctgggtgga tgctaaactg gatcgtattg atgagttgat gcagaaactc
     6601 ggcctctctt atgacgatga cgaagaagag gaagaagacg agaagcaaga agacatgatg
     6661 cgtctgctgc ggggcaacta acggattgcc gccgtgggcc ttcccgttct gctgataacc
     6721 cttccggtta tatgttatct ggtgtggtta ttcgttaaac tacagcggtt gtcgcggcga
     6781 caaaagtggc tgcgcaaccg gctgatgacc cgaaacgggc atccgccggt acgccgtagc
     6841 cgccagagac gccatcggaa ggagtgagca tgtctgtaca gcaaatcgac tgggatctgg
     6901 ccctgatcca gaaatataac tattccgggc cacgatacac ctcgtacccg accgcgctgg
     6961 agttttcaga agacttcggc gaacaggcgt ttttacaagc cgtggcgcgc tatcctgagc
     7021 gtccattatc tctctacgta catatcccgt tctgccataa gctttgttac ttctgcggtt
     7081 gcaataagat tgttactcgc cagcagcaca aggccgatca gtatctggac gcgctggagc
     7141 aagaaatcgt ccatcgtgca ccgctgttcg ccgggcgtca cgtcagccaa ttgcactggg
     7201 gcggcggaac gccgacgtat ctgaataaag cgcaaatcag ccgcctgatg aagctgctgc
     7261 gcgaaaactt ccagttcaat gccgatgcgg agatttcgat cgaagtcgat ccgcgggaaa
     7321 tcgaactgga tgtactcgat catttacgcg ccgaaggctt taatcgcctg agcatgggcg
     7381 tgcaggactt caacaaagaa gtgcaacgtc tggttaaccg cgagcaggat gaagagttca
     7441 tctttgcact gcttaaccat gcgcgtgaga ttggttttac ctccaccaac atcgacctga
     7501 tttacggcct gccgaaacag acgccggaga gtttcgcctt taccctgaaa cgtgtggcgg
     7561 agctgaaccc cgatcgtctg agtgtcttta actacgcgca tctgccgacc atttttgctg
     7621 ctcagcgcaa aatcaaagat gctgacctgc cgagtccgca gcaaaaactc gatatcctgc
     7681 aggaaaccat cgccttcctg acgcaatcgg gctatcagtt tatcggtatg gatcactttg
     7741 cccgtccgga tgacgagctg gcggtggccc agcgtgaagg cgtgctgcat cgtaacttcc
     7801 agggctacac cactcagggc gataccgatc tgctggggat gggcgtttcc gccatcagca
     7861 tgattggcga ctgctacgcg cagaaccaga aagagttgaa gcagtactat cagcaagtgg
     7921 atgaacaagg caatgcgctg tggcgtggta ttgcgctaac gcgtgatgac tgtattcgcc
     7981 gcgatgtgat taagtcgctc atctgcaact tccgtctgga ttacgcccct attgagaaac
     8041 agtgggattt gcacttcgct gattactttg cggaagatct caagctgctc gccccgttag
     8101 caaaagatgg gctggtggat gtggatgaga agggaataca ggtgacggcg aaaggtcgct
     8161 tgctgatccg caacatttgc atgtgctttg atacctatct gcgccagaaa gcgcggatgc
     8221 agcagttctc acgggtgatt tgaatagtga atggcgcttc gtttacaaag tagaaaacga
     8281 aagcgccatc aatgtgctga gaacaagatt gcctgatgcg ctacgcttat caggcctaca
     8341 aaacctgttg aaaatattgc ttttgtaggc cggataaggc gttcacgccg catccggcat
     8401 gaacaaagag cacttcatca gcaatctgaa actcaactaa acagcccaat cattgcggca
     8461 cacagcacgg cagcaacggc agtttgcggt gtgtggctgt caactgcgcc actcgatacc
     8521 agattaatta ttgattccag cataatgact ctccccgttt tccgggcaga tcatactgaa
     8581 cttatcggaa cagtaaagcg taaaatacca gcaatttgcg ctcaataatc aatctttaca
     8641 cacaagctgt gaatcactcc atccccagct cttttaactt acgcgtcagg gtgttgcggc
     8701 cccagccgag tagccgcgcc gcttcctgtt tatgcccctg cgtatgtcgc aacgcggtcg
     8761 tcagtaacgt ccgctccagc tctggctgcg cttcggaaag cagattttga tgaccggaac
     8821 gcagcgctct gtctgcccac tgcgcaagaa gcgtcgccca gctgtccggt tgcatttgcg
     8881 aagtactctc cgcaaccgtt gattcaaaca gttcgccggg caaatcctga atcaacactt
     8941 cctgcccggc ggccatcacc gttagccagc ggcaggtgtt ttccagctgg cgcacgttgc
     9001 ctggccacgc cagacgcgtc agagcagctt cggtttccgg atgcagtaac ttcgcttcta
     9061 cgcccagttc gcgcgcggca acctgtaaaa aatggcgcgc cagacgggga atatcttccc
     9121 gacgttcgcg cagcggcggc agatgaacgc ggataacgtt taggcggtgg aacagatcct
     9181 cacggaactt accttcctgc actcgctgtt cgagattctg gtgagtggca gcgataatcc
     9241 gcacatccac tttcaccggc gcatagccgc caacgcggta aaactgaccg tctgccagca
     9301 cgcgcagcaa acgcgtctgc acatccagcg gcatatcacc aatttcgtcg aggaataatg
     9361 taccgccatc ggcctgttca aaacgcccct gacgaatggt attcgcgcca gtaaacgcgc
     9421 ctttctcgtg gccaaacagt tctgattcga tcaaatcttt tgggatagct gccatattca
     9481 gcgcgataaa cggtgctttg gcgcgcggac tgtggcgatg cagggcatga gcgaccagtt
     9541 ctttaccggt gccggattcg ccgttaatca gcacgctaat agaagaacgc gaaagccgac
     9601 cgataatacg gaacacgtcc tgcatggctg gcgcttcgcc gatgatatcg gtcgttgggc
     9661 cgttaagctg aatattacgc ggctgctgct gttcctggta atgactgata gcgcgctcaa
     9721 ccagcgccac ggcttcgtcg atatcaaacg gtttgggcag ataatcaaac gccccttgtt
     9781 gataggcgct gacggcagca tccagatcgg aatgtgcggt cataatgatg accggaagca
     9841 ttggatggcg ctgtttaatc tgcttgagca gcgccagccc gtccattccc ggcatacgga
     9901 tatctgaaag cagcacatcc ggcgttttgc tcgccagcgc ctccagtact tccgcgccgt
     9961 tctcaaatgt cgtacaggtt aaacccgctc cagcgagcgc acgttcaagc acccaacgga
    10021 tggaactatc gtcatcgact acccagacta tccctcgttg cataaacgtc acctttattt
    10081 cctgataggc aggtaaaccg agaactcggt atgacctggc caactggtaa attcaatttt
    10141 gcctgaatgc tgatcaatca aattacgggc gatggataag ccaagcccgg tgccaccttc
    10201 gcggccgctg accatcgggt aaaacagcgt atcctgcaaa tgaggcggaa tgcccggccc
    10261 gttatcttcc acatcaatcc gcgccgccag ccggtagcgc tcgccgtgta aggtcagttg
    10321 aaacgcggtg cgggtacgca gaatgatttc accgccttcc ggccccagcg cctgtagcgc
    10381 attgcgcaca atattcagca agacctgttc aatttgatcc gggtcgtgcg ccagttccgg
    10441 caggctggga tcgtaatcac gaatcaaccg cacgttgtcc ggcagttcca tcgacaccag
    10501 cgttaccacg cgttcagcca ctttgtgaat actttcggta acgcgcgtac cgggcagctg
    10561 cggccccaac agacggtcga ccagatttcg cagccggtcc gcctgttcga taatcacttt
    10621 ggtatattcg agtagtgatg ggtcaggtaa cgctttgctg agcagctgcg ccgcgccacg
    10681 taaaccgcca agcggatttt taatctcatg tgccaggccg cgcactaaat cacgggcagc
    10741 aacctgctgg gcgtgctgta gctgttcctg acttaagcgg cgctggttat ccatcggagc
    10801 catctccagc aggatcatgc cgtccggcat acgctgggcc gtcacagaaa ggatatgcga
    10861 gcgcccgtcg atgaccagcg tcacttcgtt atcggtaaaa ccttgccccg cctccagact
    10921 ttcttgcatc agctcgatat ttaatgagaa gtagctcaac agttccggta acggtgtacc
    10981 aaacaatttg cgggagcttt gggcgagcag ttgttgcgcg gcagggttgg cgtaatggat
    11041 ctccaggttg tcatcgatta acaaaatact gttaatcagc gagttgagga tctgcccagc
    11101 atcgggctgc gtgcctgttg ccataaagca gtctcctgaa caggttgcac cattttagtg
    11161 cattatagct ttttacggat aaaaagcgcg aagcatcaga gaattgacgg agaaaaaagc
    11221 ccatgcagag atgggctgaa agtttccacg gcaactaaat cccggcgttg ttgcgccggg
    11281 tagtacaact tcaactatta gacgctgtag tacagctcaa actctaccgg atgcggagtc
    11341 atacgcacgc ggtcatcttc ttcgcgacgc agagcgatgt acgcatcaat tgcttcgtca
    11401 gtgaacacgc caccggcttt caggaactcg cggtccagat ccagttcgtt cagtgcttct
    11461 tccagagagc ctgcaacctg tgggatctct ttcgcttctt ctggcggcag gtcatacagg
    11521 tttttgtcca tggcttcacc cggatggatc ttgttcttga taccatcaag accggccatc
    11581 agcagggcag caaagcacag gtacgggtta gctgccggat ccgggaaacg tacttcgata
    11641 cgacgtgctt tcggagaaga aaccaccgga atacggatag acgcagaacg gttacgcgca
    11701 gagtaagcca gcattaccgg tgcttcatag cccgggacca gacgcttata agagttggtg
    11761 gtcgggtttg ccagggcgtt aatcgcttta gcgtgtttga ttacgccgcc aatgtagtac
    11821 agcgcctgct cagacagacc tgcgtatttg tcgcctgcga acaggttaac gccgttttta
    11881 gacagagaca tgtggcagtg cataccggag ccgttatcac cgaacatcgg ttttggcata
    11941 aaggtcgcgg ttttaccgaa gcggtgcgct acgttgtgca caacatattt gtagatctga
    12001 atttcgtcag cttttttggt catggtattg aagcgggtag ccacttcgtt ctgaccagca
    12061 gtcgctactt cgtggtgatg ggcttcaacc accagcccca tctgttccat caccagacac
    12121 atttcagaac gaatatcctg agccgagtct accggtggaa ccgggaagta accgcctttc
    12181 actgccggac ggtgaccttt gttaccacct tcgtattggg tggaggagtt ccatgcgcct
    12241 tcgatatcgt cgatagcaac gtgggaaccg gagatagatg atccgaaacg gatgtcatcg
    12301 aacaggaaga attcaggttc tggcccgaac agtacggtgt cggcaatgcc agtggaacgc
    12361 aggtaatctt cggcgcgctt cgcaatggag cgcgggtcac ggtcatagcc ttgcagggtg
    12421 ccaggttcaa ggatgtcgca acggataatc agggtggagt cggcgaagaa cgggtcaatc
    12481 actgcggtgg atgcgtctgg catcagcacc atgtcggact cgttaatgcc tttccagccg
    12541 ccaatcgagg agccgtcaaa cattttgcct tcttcgaaga attcagcatt cacctgatga
    12601 gcagggatag tgacgtgctg ttctttacct ttagtatcgg tgaagcgcaa atcaacaaac
    12661 ttcacttcgt gctcgttcag catcgtcagt acgtgttcag cggacatact ttaactctcc
    12721 tggattggtc atggtcgtcg tggtaacgaa atctgcaatt attttggccg tgtcgccgta
    12781 aaaaagataa agcgaaatct gtgccaactt ttaaattgcc cctaaaaggc gttatcatgc
    12841 gcaccatcgt gcaaaagggc tgcaccacga tgtgaatgtt gcaccaatat agtgcttcaa
    12901 tggaaacatt aagcaccatg ttggtgcaat gacctttgga taaccctttt tatgctccgt
    12961 gaaagcgatc acaaagggac tctgcaatac ttgtttgcgg aggatgtttg tgatcctgtt
    13021 ttgtagtgcg attaatccgt gtacaataac gcgctatttc taatgcctga ggcaaagttg
    13081 tgatcgaaaa attgcgtaat atcgccatca tcgcgcacgt agaccatggt aaaaccaccc
    13141 tggtagacaa gctgctccaa caatccggta cgttcgactc tcgtgccgaa acccaagagc
    13201 gcgtgatgga ctccaacgat ttggagaaag agcgtgggat taccatcctc gcgaaaaaca
    13261 ccgctatcaa atggaatgat taccgtatca acatcgttga taccccgggg cacgccgact
    13321 tcggtggtga agttgaacgt gtaatgtcca tggtagactc agtgctgctg gtggttgacg
    13381 catttgacgg cccgatgccg caaacgcgct tcgtaaccaa aaaagcgttt gcttacggcc
    13441 tgaagccgat tgttgttatc aacaaagttg accgccctgg cgcgcgtcct gattgggttg
    13501 tggatcaggt attcgatctg ttcgttaacc tcgacgcgac cgacgagcag ctggacttcc
    13561 cgatcgttta cgcttctgcg ctgaacggta tcgcgggtct ggaccacgaa gatatggcgg
    13621 aagacatgac cccgctgtac caggcgattg ttgaccacgt tcctgcgccg gacgttgacc
    13681 ttgacggtcc gttccagatg cagatttctc agctcgatta caacagctat gttggcgtta
    13741 tcggcattgg ccgcatcaag cgcggtaaag tgaagccgaa ccagcaggtc actatcatcg
    13801 atagcgaagg caaaacccgc aacgcgaaag tcggtaaagt gctgggccac ctcggtctgg
    13861 aacgtatcga aaccgacctg gcggaagctg gcgatatcgt tgcgatcacg ggccttggcg
    13921 aactgaacat ttctgacacc gtttgcgaca cgcaaaacgt tgaagcgctg ccggcactct
    13981 ccgttgatga gccgactgtt tctatgttct tctgcgttaa cacctcgccg ttctgcggta
    14041 aagaaggtaa gttcgtaacg tctcgtcaga tcctggatcg tctgaacaaa gaactggtac
    14101 acaacgttgc gctgcgcgta gaagaaaccg aagacgctga tgcgttccgc gtttctggtc
    14161 gtggcgaact acacctgtct gttctgatcg aaaacatgcg tcgtgaaggt ttcgaactgg
    14221 cggtatcccg tccgaaagtt atcttccgtg aaatcgacgg tcgtaaacaa gagccatatg
    14281 aaaacgtgac tctggacgtt gaagaacagc atcagggttc tgtaatgcag gcgctgggcg
    14341 aacgtaaagg cgacctgaaa aacatgaatc cagacggtaa aggccgcgta cgtctcgact
    14401 acgtgatccc aagccgtggt ctgattggct tccgttctga gttcatgacc atgacttccg
    14461 gtactggtct gctgtactcc accttcagcc actacgacga cgtacgtccg ggtgaagtgg
    14521 gtcagcgtca gaacggcgta ctgatctcta acggtcaggg taaagcggtc gcgttcgcgc
    14581 tgttcggtct gcaggatcgc ggtaagctgt tcctcggtca cggtgcagaa gtttacgaag
    14641 gtcagattat cggtattcat agccgctcta acgacctgac tgtaaactgc ctgaccggta
    14701 agaaactgac caacatgcgt gcttccggta ctgacgaagc cgttgttctg gttccgccta
    14761 tccgcatgac tctggaacaa gctctggagt tcatcgatga tgacgaactg gtagaagtga
    14821 ctccgacctc tatccgtatt cgtaaacgtc acctgacgga aaacgatcgt cgtcgcgcca
    14881 accgcgcacc gaaagactat taatttcgtt ttttagttat aaaaaacctg ccatcgctgg
    14941 ctaatgccag tcagttaagc aactgactgg ctctttttcg gggctgtggg gtatttccag
    15001 ggcctctcct ttaccactct cgggaaggcc ctt
//
LOCUS       DAJTMC010000096        14624 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.096, whole genome shotgun sequence.
ACCESSION   DAJTMC010000096 DAJTMC010000000
VERSION     DAJTMC010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14624)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14624)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14624
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.096"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(<1..1264)
                     /locus_tag="OO130_002638"
     CDS             complement(<1..1264)
                     /locus_tag="OO130_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HCR5365992.1"
                     /translation="MSTTTRKFKTIITDTGAKKLAQVAAPDGKPVRLTHMAVGDGGGT
                     LPTPDSKQTRLVHEVWRHTVNRVILDATHQNRIIAELVIPPETGGFWIREIGVFDEHG
                     DLIAVGNTAESYKPAVAEGSGRAQTFRTILTVSSTATVALTVDNTMVMATVDYVDDKL
                     KEHEQSRRHPDASLTAKGFVQLSSATNSVSETQAATPKAVKAAYDLANGKYTAQDAST
                     TRKGLVQLSSATNSTSETQAATPKAVKAAYDLANAKYTAQDATTAQKGIVQLSSATNS
                     TSETLAATSKAVKAVMDETNKKAPLNSPALTGTPTTPAARQGTNNTQIASTAFVMAAI
                     AALVDSSPDALNTLNELAAALGNAPNFATTMTNALAGKQPKDATLTALAGLATAADRF
                     PYFTGNDVASLATLTKVGRDILAKSTVAA"
     gene            complement(1267..1797)
                     /locus_tag="OO130_002639"
     CDS             complement(1267..1797)
                     /locus_tag="OO130_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HCR5365993.1"
                     /translation="MSNSLLPPSASNFMRCAEAVGTRITDIPVDLNTLWSPDTCPVHL
                     LPYLAWAFSVDRWDRNWPEETKRQVIRDAWLIHRHKGTISALRRAIEPLGYLIRVSEW
                     WEFGGEPGTFTVEVGTLDSGVTEEMYLEMERLIADARPVSRHMTGLNIIQEIPGDIFA
                     AAATYDGEVITIYPGD"
     gene            complement(1790..2686)
                     /locus_tag="OO130_002640"
     CDS             complement(1790..2686)
                     /locus_tag="OO130_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HCR5365994.1"
                     /translation="MPAVDLSQLPEPAIIAEPDFEAILADTKAMMIASYPAEQREAVS
                     AALELESEPLNVIAQTMSFREMLLRQRVNEGARACMLSHSAGTDLDNLAGNMNTKRLV
                     ITPATDTTDAVMESDTSLRLRAQRAYDGLSVAGPSGAYEYFARSASGLVRDARAISPS
                     PANVTVSILSTEGDGTATEALLNTVRAVLNAEDTRPVADRLTVQSARIVTWRLNAKLY
                     FYPGPESEPILAAAESSFRKWLAEQGLIGQDVALSAIAAALHVHGVQRVEIIEPTQNM
                     AISDIQAARCASFTISEGGRNE"
     gene            complement(2690..3019)
                     /locus_tag="OO130_002641"
     CDS             complement(2690..3019)
                     /locus_tag="OO130_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001705832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HCR5365995.1"
                     /translation="MNPDGTGNLNDMEHLKQSVRDILTTPLASRVMRREYGSLVPDLI
                     DEPMNNTTRLQCMSAAVIALTRWEPRIALDAIDVVWKAGGRAGVTLSGTVMQTMQNVE
                     LTITLRE"
     gene            complement(3037..3603)
                     /locus_tag="OO130_002642"
     CDS             complement(3037..3603)
                     /locus_tag="OO130_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HCR5365996.1"
                     /translation="MRLLSNIIRTGIISEVDEKSWRVRVRSGELETGWLRWNTSRAGA
                     FNVWLPPSPGEQVVIACIGGNPETAMIIGSLWSDAIPAPGKSLKEIVVSAPDGAVFRY
                     DADAGALSASGMKTATLQASVSVTLDTPVVECTDLLRTATLDVTKGGKMSGNITHSGG
                     NFTSNGITVHTHKHGGVKGGSDSTGGPQ"
     gene            complement(3615..4250)
                     /locus_tag="OO130_002643"
     CDS             complement(3615..4250)
                     /locus_tag="OO130_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HCR5365997.1"
                     /translation="MDKAFTRVDETFEAIRDSLNQQAINNIARKLAQDLRRAQQARIR
                     PQKAPDGTAWTPRRRHVTRIQERIRFIWNNEARTLKNWHHDTGKYGRTITGWDEDKNS
                     IRTFYRDDIDRFLEIRTRRINQDSTKRVPMFVKLRTARYLKARADASGVTVGYSGVAA
                     RIARVHQFGERDQVAPGIFTDYPVRELLGISQADERLIYNTVLGRIAEAVR"
     gene            complement(4243..4710)
                     /locus_tag="OO130_002644"
     CDS             complement(4243..4710)
                     /locus_tag="OO130_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5365998.1"
                     /translation="MLKTDSLREAMTRSCRWCQANPEKFTIFVESGNIETTGETPSFV
                     YRYQMVMFVMDYAGELDDLTLPLLAWLSENQPQLLLNPERNQDIKFSAVINDDDSADL
                     LFTLPLRERVRITRSSQGTPQAEHLLEPKPRLPSSEGDWSHVFQDVTWGESDG"
     gene            complement(4848..5255)
                     /locus_tag="OO130_002645"
     CDS             complement(4848..5255)
                     /locus_tag="OO130_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2570 domain-containing protein"
                     /protein_id="HCR5365999.1"
                     /translation="MKLWPTLGVAFLLTAGWGTSMRLSWSLGRENARNEAQASTLKST
                     ADTLNIISAGVQDMQQVLAQLRVENQQRNQDGEVRREQLRNDIAKDECAHALPDARFT
                     DRLRRHAERATASAVSPAYTADADHTGNASPLP"
     gene            complement(5252..5644)
                     /locus_tag="OO130_002646"
     CDS             complement(5252..5644)
                     /locus_tag="OO130_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HCR5366000.1"
                     /translation="MSNTFKFSSRSEKNLQGVNPDLVKVTRRALEISEVDFGITEGLR
                     SRYRQKQLVATGKSQTMNSRHLTGHAVDVVAYIGSQMSWEWPLYEKIAAAFRQASREL
                     NIPVEWGGDWKTLKDGPHFQLPHGAYPA"
     gene            complement(5641..5964)
                     /locus_tag="OO130_002647"
     CDS             complement(5641..5964)
                     /locus_tag="OO130_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HCR5366001.1"
                     /translation="MSTIQTGITEQVIAWLFDHLPTVYAVGAAVSISALMSLYDGRTL
                     VQTVTGSLACGVLAMAVAGSLRFFGFPEDAVTFIGASIGFMGAEKARDKVIAAFNRRV
                     KEKDE"
     gene            complement(5967..6167)
                     /locus_tag="OO130_002648"
     CDS             complement(5967..6167)
                     /locus_tag="OO130_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HCR5366002.1"
                     /translation="MKVKALEGDTVDSLCFRYYGTTQGVTEKVLDANPGLCQQVFLDA
                     GQEVEMPEPEKKKREMIQLWGE"
     gene            complement(6167..6661)
                     /locus_tag="OO130_002649"
     CDS             complement(6167..6661)
                     /locus_tag="OO130_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HCR5366003.1"
                     /translation="MTIVARTEPGPAEDDITDTDDGDTRISAGAFWPDIVLRELRLAV
                     RLPGRVTTSRLLHTATGAVAHVTRELEAWQQEQQAAGHQTLADVPAPVINGESVNLWH
                     WRNAVYTATRALILERYRDADTTDKGDRRADALDIQTSDLWRDVSWAISDILCRPRIF
                     AELC"
     gene            complement(6829..7575)
                     /locus_tag="OO130_002650"
     CDS             complement(6829..7575)
                     /locus_tag="OO130_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004983471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HCR5366004.1"
                     /translation="MITPAQQHWQNVMAQRAGRANEGVDHAARTAHEEVLYRLRLAQA
                     RLKGVQARSAKAAIKKELLPDFSGWIEGTLEADGGQQDEVIATLMVWAIDCGDLPLAL
                     RIGAYVVRHNLIMPDNFGRTAATVLTEEICNPVLTQAGTDADADLSAFIEPLDTLREI
                     VTDQDMPDEVRAKLCKACAFARRGLSDADSMALSLKLLREAMHLNPNAGVKREIATLS
                     RALKKADSAAEPEDASAQQAQDESSKKQQK"
     gene            complement(7621..8493)
                     /locus_tag="OO130_002651"
     CDS             complement(7621..8493)
                     /locus_tag="OO130_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005136493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P2 family phage major capsid protein"
                     /protein_id="HCR5366005.1"
                     /translation="MIGVTDQKGEKILLDTTGPIARTNTSYDGTKRRNPNNVVDLKNR
                     KYQCEQVNYDTFISYPQLDAWSAHPDFQSRISAQIARQVALDRIMIGFNGTSHADESN
                     FSTNKLLQDVNVGWLEHIRTDASERVMNDVTLTSRNMDNTVAHAGKYANADALVQDAR
                     SSLLDEWHKEADDLVVIMGRNLFNSLRLPVLNSISGQNPNAELLAGQLILSSRTIGGL
                     GVFLAPFFPDATMLITSFNNLSIYWQKGSMRRLMKDEPEYNRIATYQSINDAYVVEDY
                     GKCAMVTGLKFADS"
     gene            complement(8490..8672)
                     /locus_tag="OO130_002652"
     CDS             complement(8490..8672)
                     /locus_tag="OO130_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001344562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P2 family phage major capsid protein"
                     /protein_id="HCR5366006.1"
                     /translation="MNLVMSDITRNKLGCYMAQQVSLNNIPVSALVSRFTVEPAVQQR
                     FENASKESTEFTKKIT"
     gene            complement(8696..9532)
                     /locus_tag="OO130_002653"
                     /pseudo
     CDS             complement(8696..9532)
                     /locus_tag="OO130_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262634.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
     gene            9687..11438
                     /locus_tag="OO130_002654"
     CDS             9687..11438
                     /locus_tag="OO130_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HCR5366007.1"
                     /translation="MIQDAFVRQRARQLYWQGYPPAEISRLMGINPNTIYAWKKRDQW
                     DETPPGQRVTQSIDARLIQLTEKQNKTGGDFKEIDLLTRQLKKLHDGQPDVMAAGKKG
                     RAKKLKNHFTPEQIAALREKIISRLEWHQRGWFDSLTLCREAGIRNRMILKSRQIGAT
                     WYFAQEALLMALRDDVAQPYQRNQIFLSASRRQAFQFKSIIQKAAAEVDVELKGGDKI
                     ILSNGAELHFLGTSAASAQSYTGNFYFDEFFWVSRFAELRKVAGAMATLSGLRRTYFS
                     TPSTETHEAYAYWNGDRWNEKKASHKRQRFSVDWKTLHNGLICPDRTWRQIVTLEDVV
                     NHGWKHTDIDEIRDENTEDEFLNLYMCEFVREGESAFNLNILIGCGVDGYDDWKDWKP
                     FAPRPMGNRPVWIGYDANGSSGNGDSGAVSVVVPPSVPGGRFRTVETRRVQGLEFEEQ
                     ARVIEEFTCRYNVEHIGIDVTGGNGEAVYQIVKRFFPAAIPYTFTLSSKRSLVLKMLQ
                     IMRAGRWEYDRAERELVAAFNAVRKVKTPGGFITYETDRARGISHGDLAWATMLAVIN
                     EPIGGEGENERFTVMEF"
     gene            11438..12474
                     /locus_tag="OO130_002655"
                     /pseudo
     CDS             11438..12474
                     /locus_tag="OO130_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000087802.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            complement(12953..13543)
                     /locus_tag="OO130_002656"
     CDS             complement(12953..13543)
                     /locus_tag="OO130_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HCR5366008.1"
                     /translation="MSEINYQALRERYSPVPVPKCPICGEEMSIQRISGAQVVYACSG
                     YGDDGDFKIGRTLADKHYEKSRVTVLDVGDPEVLALLDWLETKDNRIAELEKIATDYA
                     LKFQKAQDALKHAALLHSRTAQQTNNFAVSLPDISEYFINDVFQPLRYERDVERAIIK
                     AGGKALWQEKHEDRTHQSCDVNCGWFSPLTTDKNNT"
     gene            complement(13607..13918)
                     /locus_tag="OO130_002657"
     CDS             complement(13607..13918)
                     /locus_tag="OO130_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012907691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HCR5366009.1"
                     /translation="MTTPTRRISFYLKPTAVKNEGEACAWLDSLTPEARKSGQRVAFL
                     AGLALLKMNPAEAYRLAAWAGDEALSVTQTRTERPASQPVSTAQITSQMAGNIRALFP
                     E"
     gene            complement(13923..14528)
                     /locus_tag="OO130_002658"
                     /pseudo
     CDS             complement(13923..14528)
                     /locus_tag="OO130_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310661.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
BASE COUNT         2972 a         4067 c         4029 g         3556 t
ORIGIN      
        1 cggcagcaac ggtcgatttc gcaagaatat cccgcccgac ttttgtcagg gttgccaggc
       61 tggcgacatc attccccgta aaatacggaa acctgtctgc cgcagtagca agcccggcca
      121 gcgccgtcag ggtggcatct ttcggttgct tacccgcaag cgcgttagtc atggtggtcg
      181 caaaattcgg ggcgtttccc agcgccgccg ctaactcgtt cagcgtattc agtgcgtcag
      241 gtgacgaatc tacaagggcg gcaatcgcag ccataacgaa agccgtgctt gcgatttggg
      301 tattattcgt tccctgtcgc gcagctggcg ttgttggcgt tccggtcagc gcaggactgt
      361 ttaagggcgc tttcttgttc gtttcatcca ttaccgcctt aaccgctttc gatgtcgcgg
      421 ccagtgtttc agacgtgctg ttggtggcac tactgagctg gactatccct ttttgtgccg
      481 tcgtggcgtc ctgagcggta tattttgcgt tagcaaggtc atacgcggcc tttactgctt
      541 tcggcgttgc agcctgcgtt tcagacgtgc tgttggtggc gctactgagc tggacaaggc
      601 cttttcgcgt cgtgctggca tcctgcgcag tatatttacc gttagcaaga tcatacgcgg
      661 cctttactgc tttcggcgtt gcagcctgcg tttcagacac gctgttagtg gcgctactga
      721 gttgaacaaa gccttttgcg gtcagcgagg cgtccgggtg acgtcgtgac tgttcatgct
      781 ctttcagttt gtcatccacg taatccactg tggccatcac catggtgttg tccacggtaa
      841 gcgccacggt ggcagtgctg gatacggtca gaatggtgcg aaatgtttgt gcacgaccgg
      901 acccttcggc aacggctggc ttgtaacttt cggcagtatt tcccaccgcg attaaatcgc
      961 cgtgctcatc aaatacacca atttcccgga tccagaatcc gcccgtttca ggaggaataa
     1021 ccagctccgc aataatgcgg ttctgatgtg ttgcgtccag gatgacgcga ttaacagtat
     1081 gtcgccacac ctcatgcacc agacgggtct gcttactgtc tggtgtgggc aacgtgccgc
     1141 caccgtcgcc cacggccata tgagtcaggc ggacaggctt accatctggc gcggctacct
     1201 gagctaattt ttttgcaccc gtatcggtga taatggtttt aaattttcgt gttgtggtac
     1261 tcatgcttaa tcgcctggat aaatggtaat aacttcaccg tcataagttg ccgccgctgc
     1321 gaaaatatcc cccggaattt cctgaatgat attcagccct gtcatgtggc ggctgaccgg
     1381 gcgggcatca gcaatcaacc gctccatttc cagatacatt tcctccgtca cgccactgtc
     1441 cagcgtgccg acttcaacgg taaatgttcc cggttctccg ccgaactccc accactcaga
     1501 cacgcgaatg aggtatccca gcggctcaat ggcccggcgc agtgcgctga tggtcccttt
     1561 gtgtcggtgt atcagccatg catcacgaat aacctgtcgc tttgtctctt ccggccagtt
     1621 gcgatcccag cggtcaacgg aaaacgccca ggcgagataa ggcagcagat gcaccgggca
     1681 ggtgtccggc gaccacagcg tgttgaggtc taccggaatg tctgtaatgc gtgttccgac
     1741 ggcttcggca caacgcatga aattgctggc tgatggtggt aacaacgaat tactcattgc
     1801 gtccaccttc gctgatggtg aatgacgcac agcgcgccgc ctgtatgtcg ctgatggcca
     1861 tattctgtgt gggttcgatt atctccacgc gttgcacacc gtgcacatgc agtgcggcag
     1921 caatggcgga caacgccacg tcctgaccga taagcccctg ctcagccagc cacttcctga
     1981 acgacgattc agccgcggcc agaataggtt cggattccgg gccggggtaa aagtacagtt
     2041 ttgcattcag ccgccatgtc acgattctgg cactctgtac ggtcaggcgg tcggccaccg
     2101 ggcgggtatc ctctgcattc agaacggcgc gaacggtatt aagcaacgcc tccgttgctg
     2161 tgccgtcgcc ctcagtggac aggatggaaa ccgtcacgtt ggccggagac gggctgatgg
     2221 cccgcgcatc gcgtaccaga ccgctggcgc tgcgtgcaaa atactcgtat gcacctgacg
     2281 ggccagcaac actcagaccg tcatatgccc gttgcgcccg tagtctcagc gaggtgtcac
     2341 tttccatcac tgcatcggtg gtatccgttg ccggagtgat aaccaggcgc tttgtgttca
     2401 tattgcccgc gaggttgtcc aggtctgtcc cggcgctgtg gcttagcatg caggcgcgtg
     2461 caccctcatt gacccgctgg cgtaacagca tttcacgaaa cgacatggtt tgagcgataa
     2521 cgttaagggg ttccgattcc agctccagcg cggcggaaac ggcttcacgc tgttcggcgg
     2581 gataggacgc aatcatcatg gcctttgtgt cagccagaat tgcctcaaag tcaggctccg
     2641 cgatgatggc gggttccggt aactgtgaaa ggtcaacggc aggcatgatt tactccctca
     2701 gcgtgatggt taattcaaca ttctgcatgg tctgcatgac agtgcccgac agcgtcaccc
     2761 cggcgcggcc tcctgccttc cagacaacgt cgatagcgtc cagggcaatg cggggttccc
     2821 atcgtgtcag cgcaatcacg gcagcactca tgcattgcag acgcgtggtg ttattcatgg
     2881 gttcgtcaat caaatcaggc acaaggctgc catattcccg tcgcataacc cggcttgcca
     2941 gcggggtggt caggatgtcc ctgactgact gtttcaggtg ctccatatcg ttcaggtttc
     3001 ccgttccgtc cgggttcatt cctgtgtagc gggttgtcac tgcgggcctc ctgtcgaatc
     3061 gctgccacct ttaacgccac cgtgtttatg cgtatgcact gtgatgccgt ttgaggtgaa
     3121 attaccgccg ctgtgcgtga tattgccgct catctttccc ccttttgtga cgtcaagcgt
     3181 cgccgttctc agaaggtctg tgcattccac gacgggcgta tccagtgtca cgctgacgga
     3241 tgcctgcagg gtggctgttt tcatgccgct ggcgctcagt gcgcctgcgt ccgcgtcgta
     3301 gcggaacacc gcgccatccg gcgcgctgac cacgatttct ttcaggcttt tgccgggggc
     3361 cggaatggca tcactccaca ggctgccaat tatcatggcg gtttccgggt tgccgccaat
     3421 gcaggcaatt accacctgtt cgcctggtga tggcggcagc cacacattga aggctcccgc
     3481 gcgagaggtg ttccagcgca gccaacctgt ttccagttcg ccgctgcgaa cgcgcacgcg
     3541 ccaggacttc tcatcaactt cagagatgat cccggtgcgg atgatattgc tcagtagccg
     3601 catgagttct gcgctcaccg tacagcctcc gcaatccggc ccagcaccgt gttataaatc
     3661 aggcgctcat ctgcctggct gatacccagc agctcacgta ccgggtaatc ggtgaaaatg
     3721 cccggcgcaa cctgatcgcg ctcaccgaac tgatgaacgc gtgcaatacg tgcggccacg
     3781 ccgctgtaac ccaccgtcac accggaagca tctgcacggg ctttcaggta gcgggcggtg
     3841 cgcagtttta cgaacatggg gacgcgcttt gtgctgtcct ggttgatgcg ccgggtgcgt
     3901 atttccagaa agcggtcgat gtcatcccgg taaaacgtgc ggatgctgtt tttatcctca
     3961 tcccacccgg tgatggttcg cccgtatttc cccgtgtcgt gatgccagtt tttcagcgtg
     4021 cgtgcttcgt tattccagat aaagcgaatg cgctcctgta tccgggttac gtggcgtctg
     4081 cgtggtgtcc atgcggtccc gtccggcgct ttctgtggcc ggatacgtgc ctgctgggcg
     4141 cggcgtaaat cctgtgccag ctttctggcg atgttattga tggcctgctg attcaggctg
     4201 tcgcggatgg cctcaaaggt ttcatccacg cgagtgaatg ccttatccat cgctttcacc
     4261 ccacgtcaca tcctggaata catgcgacca gtcgccttcg gaagatggca ggcggggttt
     4321 tggctccagc aggtgttctg cctgcggtgt gccctgactg ctgcgcgtga tgcgaacgcg
     4381 ttcccgcagg gggagcgtaa acaggagatc ggcgctgtca tcgtcattga taacggcgga
     4441 aaatttgatg tcctgattac gctcagggtt gagcaacaac tgtggctgat tttcggataa
     4501 ccacgccagc agcggcagcg tgaggtcgtc cagctccccg gcgtaatcca tgacaaacat
     4561 caccatctga tagcggtaaa caaacgatgg ggtttctccg gtcgtttcaa tgttgccgct
     4621 ctccacgaaa atggtgaatt tttccgggtt agcctgacac catcggcatg aacgggtcat
     4681 ggcttcacgc agggaatcag ttttcagcat ggttgttatc ctcgttgttc agtcgttgca
     4741 gcctgcgctg ttccagtaat tcaatggccc gtttatccgc gttacaggtt tccagtgcat
     4801 ccagaaggcg gtcgccccat ataccgagat ttccccatgt gggagtgtca gggaaggggg
     4861 gaggcgttac cggtatggtc agcgtctgcg gtataagccg gactgacggc gctggcagtg
     4921 gcgcgttctg cgtgcctgcg cagcctgtca gtaaaacgag cgtcaggcaa agcgtgggcg
     4981 cattcatctt ttgcaatatc gttgcgtagc tgttcacgtc ttacctctcc gtcctgattg
     5041 cgttgctgat tttccacgcg gagttgcgcc agcacctgct gcatatcctg taccccggcg
     5101 ctgatgatat tcagggtgtc ggcggtactt ttcagggtgc tggcctgcgc ttcgtttctg
     5161 gcgttctccc ggcccagcga ccacgacaga cgcatggatg ttccccatcc ggcagtcaga
     5221 aggaaagcga cgccaagcgt gggccagagc ttcatgccgg ataggctccg tgtggtaact
     5281 gaaaatgcgg tccgtctttc agggtcttcc agtcgccgcc ccattccacc ggaatattca
     5341 gttcccggct ggcctgtctg aatgctgctg cgattttttc gtacagcggc cattcccatg
     5401 acatctggct gccgatataa gccacaacat ccacggcatg ccccgtaagg tggcggctgt
     5461 tcatggtctg gctcttaccc gtggccacca gttgcttctg gcggtaacgg ctgcgcaacc
     5521 cttcggtgat accaaaatcc acttccgaga tttccagtgc ccgtcgggtc actttcacca
     5581 gatcaggatt tacaccctgc aaattctttt cgctccggct gctgaattta aatgtgttgc
     5641 tcattcgtcc ttctccttca ccctgcgatt aaaggccgca ataaccttgt cgcgtgcttt
     5701 ctctgcaccc ataaaaccga ttgatgcgcc gataaacgtc acggcatctt caggaaaccc
     5761 gaagaagcgc aacgacccgg ccacggccat ggcaagaacg ccgcacgcca gcgatcccgt
     5821 tacggtctga accagtgttc gtccgtcata aaggctcatc agcgcggaaa tgctgaccgc
     5881 cgcgcctact gcatacaccg ttggcaggtg gtcaaagagc cacgcaataa cctgctctgt
     5941 gatccctgtt tgaatggtgc tcactgctac tccccccaca actgaatcat ttctcgtttc
     6001 ttcttctccg gctccggcat ctccacttcc tgcccggcgt ccagaaatac ctgctgacag
     6061 agtccggggt tggcatccag caccttttcg gtgacgccct gcgtcgtgcc gtagtaccgg
     6121 aaacagagcg aatccacggt gtcgccttcc agtgccttca ctttcatcag cacaactccg
     6181 caaagattcg cgggcggcac agaatgtcag agatggccca gctcacatca cgccacaaat
     6241 ccgatgtctg tatatccaga gcgtctgccc ggcggtcgcc cttgtccgta gtgtctgcat
     6301 cacgataacg ctccagaatc agggcgcgcg tggcggtata aacagcattg cgccagtgcc
     6361 agagattgac gctttctccg ttaattacgg gtgccggaac atcggccagc gtctgatggc
     6421 cagctgcctg ctgttcctgc tgccatgctt ccagctcgcg ggtaacgtgt gccacagccc
     6481 cggtggcagt atgcagcagg cgggaggtgg tcacgcgccc cggcagtcgt accgccagac
     6541 gcagctcgcg cagcacaata tccggccaga atgcacccgc tgaaatgcgg gtatcgccat
     6601 catcggtatc ggtgatgtcg tcctctgcgg gtccgggttc agttctggca actatagtca
     6661 tggggttcac tcctgaaaaa aaatcgggcg gtgggtgcgc ggtgtaaacg gtcacggagt
     6721 caaactggaa caccgcgcac gccgcccgct gacggggtca gtcgttaacc gcgcttcgcc
     6781 ttctgcgtcg cggtggtttt tcgtgttgca ggcttccgcg ttgtcttttt acttttgctg
     6841 ctttttgctg ctttcgtcct gcgcctgctg tgcgctggcg tcttctggtt cggctgcgga
     6901 atcggctttt ttcagggcgc gggaaagggt tgcaatctcg cgtttcacac ctgcgttcgg
     6961 gttcaggtgc attgcttcgc gcagcagctt cagtgacagg gccatgctgt ccgcatcact
     7021 caggccacgg cgggcaaagg cgcacgcttt gcataatttg gcgcgcactt cgtccggcat
     7081 gtcctggtcg gtgacaatct cccggagggt gtccagtggt tcgataaagg cggacaaatc
     7141 cgcgtcggca tccgtcccgg cctgcgtcag taccggatta cagatttctt cggtcagtac
     7201 cgtggcagca gtacggccaa agttatccgg catgatgagg ttgtgacgga ctacatacgc
     7261 gccgatacgc agcgcaagcg gaagatcgcc acagtcaatc gcccacacca tcagcgtggc
     7321 aatcacttca tcctgctgcc cgccgtcagc ctccagcgtt ccctcaatcc agccggaaaa
     7381 atccggcaac aactcttttt tgatggcggc tttcgcgctt ctggcctgta cgcccttaag
     7441 ccgggcctgt gccagacgca gacgatacag cacctcttca tgcgcggtac gcgcggcgtg
     7501 gtccacgcct tcattcgccc ggcctgcgcg ctgtgccatc acgttctgcc agtgttgttg
     7561 tgcaggagta atcatttttg ctctccgtta caggcgggca tgatgcccgc cgtgagttga
     7621 ttagctgtcg gcgaacttca ggccagtaac catcgcgcac ttgccatagt cttcaacgac
     7681 ataagcgtca ttgatggact ggtaggtggc gatgcggttg tattccggct cgtctttcat
     7741 caggcgacgc attgaacctt tctgccagta aatcgacagg ttgttgaacg aggtgatcag
     7801 catcgttgca tccgggaaga acggcgcaag gaacacgccc agcccgccaa tggtgcgcga
     7861 tgacaggatg agctgcccgg cgagtaattc cgcattggga ttctggccgc tgatgctgtt
     7921 cagcacgggc agacgcagcg agttaaacag gttgcgcccc ataatcacca cgaggtcgtc
     7981 agcttccttg tgccattcat ctagcaggga tgaacgcgcg tcctgtacca gtgcatcagc
     8041 gttcgcatac ttacccgcgt gcgccacggt gttgtccatg ttgcgggagg tcagcgtcac
     8101 gtcattcata acgcgttcgc tggcgtcggt tctgatgtgc tccagccagc ccacgttaac
     8161 gtcctgaagc agcttgttgg tgctgaagtt ggactcatcc gcgtgagacg tgccgttgaa
     8221 accgatcatg atgcggtcaa gcgccacctg tcgggcaatc tgtgcgctga tgcgggactg
     8281 aaaatcaggg tgtgccgacc aggcatcaag ctgcggatac gaaataaacg tgtcgtagtt
     8341 cacctgttca cactgatatt tgcggttttt cagatcaacc acgttattcg ggttacggcg
     8401 ttttgtgccg tcataactgg tattcgtgcg cgcaatcggt cctgtggtat ccaggaggat
     8461 tttttcgcct ttctggtcgg tcacgccgat cacgttattt ttttcgtaaa ttcggtactt
     8521 tcctttgagg cgttttcaaa acgctgctgc accgcgggtt ccacggtaaa tcgcgacacc
     8581 agtgcagata ccgggatatt attaagcgac acctgctgcg ccatatagca acccagcttg
     8641 ttgcgggtaa tatctgacat caccagattc ataaaaaatt tgctcctttg tctaatcaga
     8701 agtcggccag ctggtcggag gctgcgcccg ttgcggtgaa gcggttctgc ggatcgccgt
     8761 cctgcgtgcg cagtttttcc ttcagtgctg ccagctctgt ggtcagtgac gtaattttct
     8821 ggcggtcctg ctgatggcgg gtttccagca cattaaaacg gtcgataatg tcggcctgtg
     8881 acgttgcgac gccttccacc gcttcctgaa tacgggaaaa actggcgtca tccgctttgc
     8941 ggccacggcc aataatcccc attacgcggt taaaccactg ggtgccttct tcctggcgtt
     9001 gttctgccat ttcgatgatt tcagactcga tggcttcgga gataagcggc gcttcaccct
     9061 ggatactgtt aaacgtcatc accgcctaac gttgctgtgc cgtgaatttc aggcgctcag
     9121 tgcccaggct tgccggggtg tcggtcatcg ccagcccgac cagataggcg cgcccgttaa
     9181 cggagaactg cgggtgcagt tcgatactgg aatagatttt cttgccgtcc gcaacaagct
     9241 gcttcatgcg ctcggtcggt tcgatttctg catacagcgc agtacgtccg gccagcggac
     9301 cttccgtaat gtcttccgta ctcagtgcgg tgacatcgcc cattgcggaa aattcgcttg
     9361 acgggcatgg cgagagatag tgctcaacgt tcacgcgggc agcgtaaaca tccgggttga
     9421 agttctcggc ggcttcacgc agatgcaccg gactgatttc acggccatca acagttgatc
     9481 cggagacagc cacgcgaaac tttttgcggg atgtcttttt ttcattagcc atagtttttg
     9541 cccctctgac tggttcttca gtcatgatgg caaagcgtaa caggctgata caaagggctt
     9601 ttgttgtaag aaaacggcca gaacagaggg ttaaggagaa cagtttcgcg cgcgggtaat
     9661 cttcctgtaa ttactcaggg ggagcaatga ttcaggacgc ttttgtgcgc cagcgtgcgc
     9721 ggcaacttta ctggcagggt tatccgcccg cagaaatatc acgtctgatg ggaataaacc
     9781 cgaacacgat ttatgcgtgg aaaaaacgtg accagtggga tgaaacgcca cccgggcagc
     9841 gtgtcacgca gtccatcgat gcgcgcctca tccagcttac tgaaaaacag aataaaacag
     9901 gcggtgactt taaggaaata gacctgctga cccggcagct taaaaagctg catgatggcc
     9961 agccggatgt gatggccgca ggaaagaaag gccgggcgaa aaaactcaaa aatcatttca
    10021 cgccggaaca gattgccgca ctgcgggaaa aaatcatcag caggctggag tggcatcagc
    10081 ggggctggtt tgactccctg accctttgca gggaagccgg gatacgtaac aggatgatcc
    10141 tgaaatcccg acagattggg gcgacctggt attttgcaca ggaagcactg ctgatggcgc
    10201 tgcgtgacga tgtggcgcaa ccttaccagc gtaaccagat ttttttgtct gcgtcgcgtc
    10261 gtcaggcgtt ccagtttaaa agcattattc agaaggccgc ggctgaagtt gatgtggagc
    10321 tgaaaggggg cgataaaatc atcctctcca acggcgcaga gctgcatttt ctcggcactt
    10381 ctgctgcgtc ggcacagtcc tatacgggca atttttattt tgatgaattt ttctgggtca
    10441 gtcgctttgc tgaactgcgc aaggtggctg gcgctatggc aaccctcagc ggactgcggc
    10501 gcacctactt ctccacgcca tccaccgaaa cgcacgaggc atacgcctac tggaacggcg
    10561 accgctggaa cgagaaaaag gcctcgcata aacgccagcg tttttctgtg gactggaaaa
    10621 cgctgcataa cgggcttatc tgccctgacc ggacgtggcg gcaaattgtc acgctggaag
    10681 atgtggttaa tcacggctgg aaacacaccg atatcgacga aattcgtgat gaaaacaccg
    10741 aagacgagtt cctcaatctc tatatgtgtg agtttgtccg cgaaggggaa tcggcattta
    10801 acctgaatat cctgattggc tgcggcgttg acggatacga cgactggaaa gactggaaac
    10861 cttttgctcc ccgcccgatg gggaatcgtc cggtatggat tgggtatgac gcaaacggca
    10921 gcagtggcaa cggcgacagc ggcgctgtgt ccgtggtggt tcctccgtct gttcctggtg
    10981 gccgttttcg aacggtggag acgcgacgcg ttcaggggct ggagtttgaa gaacaggcca
    11041 gagtcattga agagttcacg tgtcgctaca acgtggaaca catcggcatt gatgtgacgg
    11101 gcgggaacgg ggaggctgtt tatcagatag tgaaacggtt tttccctgcc gctattccgt
    11161 acaccttcac gctgtcatca aaacggtcgc tggtactgaa aatgctgcaa ataatgcgtg
    11221 ccgggcggtg ggaatacgat cgcgccgaac gcgagctggt cgcggccttt aacgccgtgc
    11281 gtaaggtgaa aacaccgggc ggctttatca cttacgaaac ggaccgagcg agggggatca
    11341 gccacggcga ccttgcgtgg gcaaccatgc ttgctgtcat taacgaaccg attggcggcg
    11401 aaggagaaaa cgagcgtttc acggttatgg agttctgatg agcagaaaaa ataaaaaagt
    11461 gcgcatgagt tcacgcattg atctcgctga tgcgctcagg aaagaatcat cgctcagtgc
    11521 attcacattt gatggtcctt atcgcctgac ctgctggaca atatgtactg tgctgataac
    11581 gggcggtggt atgaaacccc ggtggactgg tacggtctgg caagagctgc ccggcaaacg
    11641 tcctggcatc agtctgcgct ttattttaag cgcaatgtat tgctcggttg ctacatcccg
    11701 cacccgctgc tttcccggca ggatttctcg gcgctggcgc tggactggtt tgtgttcggt
    11761 aacgcattcc ttgagcttcg aagcaatatg ctcggcgaac cgcttaaatt acggcacgcc
    11821 ctggcgaaat acatgcgacg cggaagcgat cttgaatcat ggaggtatgt gcaggatggc
    11881 aaggatgcgt ttcagtttcg cccaggcaaa gtgtgtcacc tgatgaatcc tgacattaac
    11941 caggaaatct acggcatgcc ggaatatctc ggcgcattac tctcggccag cctgtctcat
    12001 tcggcggaca tgttcagaaa actgtattac gacaacggat cccacgccgg gtgcatcatc
    12061 tacatcggtg ccgcgcaggt aaaccgcgaa agcatggact ccctgaaaga aacgctacag
    12121 ggtgcgcgtg gtggtggtgc gtttaaaaac gtgctcattc atgcgcccaa cgggggcaaa
    12181 gagggggtgc aaattttgcc gttccagcag atcaccgcaa aggatgagtt catgaatgtt
    12241 aaggcggcat cccgtgatga tgtgctggct gcgcaccgcg ttccgccgca actgatgggg
    12301 gcgatgccgg gtgaaaaaag tgcgtttggt gatgtggaga aggccgcgcg ggtttacgca
    12361 attaacgagc tgatgcccgt catggaggcc atgaagcaca tcaatgactg gcttggcgaa
    12421 gaggtgatcc gctttaaccc ttacgcactg ttagacatcc agcccacatc ctgacgcgct
    12481 tcgcttgtct gctgcttcgc cggggcataa aaaatttatg ccccgactct ccagctcctg
    12541 tatcagtcag ataatttcac gacgccttcc agtttatcgc caccatcgac ggtcagactc
    12601 ttacgcaatc ccaccgcgtt gactgcatgt tctcgccgcc tcagtgcgat tttgacggcc
    12661 ttacctttca ccccatcaaa tcagaatccc tcacgtattt ttcacgctca gcgtgagaaa
    12721 tacggccatt ctgtcgtgtc gctgcgacat cgttaaggga acgctattta ccccctgaaa
    12781 cgcgggctgt tcccccgtca cctgcgcgca gaaaaagcgc gtttttttgt gcacgcacgg
    12841 atccttgacg gatccagccg ccacgcgggc tggaagggca aaaattcgtt caaaaaaatt
    12901 gtgcaaatgt gcagcagggc gatgtgcgat tatcgccctg gtttgagggg gatcaggtgt
    12961 tatttttatc tgtcgtcagt gggctaaacc atccacaatt tacatcgcat gactgatgcg
    13021 ttctgtcctc gtgtttctcc tgccacaatg cttttccgcc agcctttatg atggctcttt
    13081 caacatcccg ctcgtatcgc aagggctgaa atacgtcatt aatgaaatat tcgcttatgt
    13141 ccggaagcga tactgcaaaa ttatttgttt gttgcgccgt cctgctatgc agcaaagcgg
    13201 catgctttaa tgcgtcctgt gctttctgga atttaagtgc atagtcagtg gcgatttttt
    13261 ccagttcagc gattcggttg tctttggttt ccagccaatc aagtagcgcc aatacttcag
    13321 gatccccgac atccaacact gttacgcgtg atttttcata atgtttgtca gcaagagttc
    13381 ggccaatttt gaaatctcca tcatcaccat aaccggagca ggcataaaca acctgtgctc
    13441 cagatattcg ctgaattgac atttcctcgc cgcaaatagg gcatttcggt actggcacag
    13501 gtgaataacg ttcacgtaat gcctgatagt tgatttcgct cattaagtcg ttctccttgt
    13561 ttatgttctg aggtggagag cagggcgaac tcgccctgat gttgtgttat tcaggaaata
    13621 acgcccggat atttccggcc atctgactgg ttatctgtgc ggttgatact ggctgtgatg
    13681 cggggcgttc tgtcctggtt tgtgtcactg ataacgcttc atcaccagcc catgcagcca
    13741 gtcgataagc ctctgccgga ttcattttca gaagtgccag cccggccaga aaagccacgc
    13801 gttggccgct tttgcgggct tctggtgtaa ggctgtccag ccaggcgcat gcttctcctt
    13861 cgttcttgac ggcggtgggc ttcagataga aacttatccg tctggttgga gtcgtcattg
    13921 gtttactcct tgtccattgc gtacagccca ttaaccagag caaactgtgg caccccgtcc
    13981 gcgatgaaag tcgcattaac tccgcaggct tcgcggatag cgggtgccac aatctccgcc
    14041 cctccaccga caaccatcac ccgcccgtaa cccgaaaaaa ccgccagcgc gcggatcacg
    14101 cgttgtttca gtgtttcttc cttttcacga attaccgcca tcaggctggc gtaatgcgcg
    14161 tcattgtgga tgtgctggcg cagccaggct tcatcatggc gatgttcgat aatggtattg
    14221 gcgatgtggt gactggtgcg cataccgtta gtggccatca ccgacagtac ggcatcggcc
    14281 atcagagaaa cgcccacgtg tggatcgcaa aacacctggc tgatacctgc cagttgcccc
    14341 tgaacctttg ccacatccag cgtggttccg cccaaatcca caatcagcag ggattcaaac
    14401 ggactcatgt cagccagtgc cttaaagcca gccggaatgg attcaggcat aacctgtacg
    14461 ttacggatag tgaatgcttc gccgttctgg tactccaccg ggcgcatgac gttcgctttt
    14521 ttgcggtttg aagtggtcaa caaaaactgg ccaccgagtt agagtttttc cagtatcgat
    14581 tttccgattc gtttgggggt aacccaccgt tatattcgtg cggt
//
LOCUS       DAJTMC010000097        14608 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.097, whole genome shotgun sequence.
ACCESSION   DAJTMC010000097 DAJTMC010000000
VERSION     DAJTMC010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14608
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.097"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(98..889)
                     /gene="fimH"
                     /locus_tag="OO130_002659"
                     /pseudo
     CDS             complement(98..889)
                     /gene="fimH"
                     /locus_tag="OO130_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418740.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
     gene            complement(909..1412)
                     /gene="fimG"
                     /locus_tag="OO130_002660"
     CDS             complement(909..1412)
                     /gene="fimG"
                     /locus_tag="OO130_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HCR5366010.1"
                     /translation="MKWRKRGYLLAAILALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGETKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(1425..1955)
                     /gene="fimF"
                     /locus_tag="OO130_002661"
     CDS             complement(1425..1955)
                     /gene="fimF"
                     /locus_tag="OO130_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HCR5366011.1"
                     /translation="MRNKPFYLLCAFLWLAVSRVLAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAASGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(1965..4601)
                     /gene="fimD"
                     /locus_tag="OO130_002662"
                     /pseudo
     CDS             complement(1965..4601)
                     /gene="fimD"
                     /locus_tag="OO130_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
     gene            complement(4668..5393)
                     /gene="fimC"
                     /locus_tag="OO130_002663"
     CDS             complement(4668..5393)
                     /gene="fimC"
                     /locus_tag="OO130_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418736.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HCR5366012.1"
                     /translation="MSNKNANVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWMENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENTLQFAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(5430..5969)
                     /locus_tag="OO130_002664"
     CDS             complement(5430..5969)
                     /locus_tag="OO130_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5366013.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAVGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIATNIGVALFDDEGNLVPINRPPVNWKRLYSGSTSLHFIAKYRATG
                     RWVTGGIANAQAWFSLTYQ"
     gene            complement(6034..6582)
                     /gene="fimA"
                     /locus_tag="OO130_002665"
     CDS             complement(6034..6582)
                     /gene="fimA"
                     /locus_tag="OO130_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HCR5366014.1"
                     /translation="MKIKTLAIVVLSALSLSSTAALAAATTVNGGTVHFKGEVVNAAC
                     AVDAGSVDQIVQLGQVRTASLAQEGATSSAVGFNIQLNDCDTNVASKAAVAFLGTAID
                     AGHTNVLALQSSAAGSATNVGVQILDRTGAALTLDGATFSEQTTLNNGTNTIPFQARY
                     YATGVATPGAANADATFKVQYQ"
     gene            complement(7063..7659)
                     /gene="fimE"
                     /locus_tag="OO130_002666"
     CDS             complement(7063..7659)
                     /gene="fimE"
                     /locus_tag="OO130_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313299.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HCR5366015.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTLERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
     gene            complement(8137..8739)
                     /gene="fimB"
                     /locus_tag="OO130_002667"
     CDS             complement(8137..8739)
                     /gene="fimB"
                     /locus_tag="OO130_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
                     /protein_id="HCR5366016.1"
                     /translation="MKNKADNKKRNFLTHSEIESLLKAANTGPHAARNYCLTLLCFIH
                     GFRASEICRLRISDIDLKAKCIYIHRLKKGFSTTPPLLNKEVQALKNWLSIRTSYPHA
                     ESEWVFLSRKGNPLSRQQFYHIISTSGGNAGLSLEIHPHMLRHSCGFALANMGIDTRL
                     IQDYLGHRNIRHTVWYTASNAGRFYGIWDRARGRQRHAVL"
     gene            10195..10911
                     /gene="nanC"
                     /locus_tag="OO130_002668"
     CDS             10195..10911
                     /gene="nanC"
                     /locus_tag="OO130_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HCR5366017.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSSDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            10931..12037
                     /gene="nanM"
                     /locus_tag="OO130_002669"
     CDS             10931..12037
                     /gene="nanM"
                     /locus_tag="OO130_002669"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HCR5366018.1"
                     /translation="MNKTITALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPRDQATSAFIDGNLYVFGGIGKNREGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAVDKINAYYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLAPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQHGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            12102..13082
                     /locus_tag="OO130_002670"
     CDS             12102..13082
                     /locus_tag="OO130_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HCR5366019.1"
                     /translation="MDAIISPDYYYVLTIAGQSNAMAYGEGLPLPAREDAPHPRIKQL
                     ARFAHTHPGGPSCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLSFIPDNAGVLIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHAYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLIMGYYGSAYRSPENWTTALRSSHFSAAARRGIISDRFVEAILQFWREK"
     gene            complement(13090..13212)
                     /locus_tag="OO130_002671"
     CDS             complement(13090..13212)
                     /locus_tag="OO130_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012139841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR5366020.1"
                     /translation="MISFVFLLLVCPNCHAMLHRRKPPFTPEELKALMDANKSN"
     gene            13294..14166
                     /locus_tag="OO130_002672"
     CDS             13294..14166
                     /locus_tag="OO130_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004186812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR5366021.1"
                     /translation="MASSKSLQQAIANIKIWHKGEQRAPHKPLLLLYVLAGYLNGHPR
                     LFDYGSEIYEPLHSLLERFGPQRSQYRPDMPFWRLQGDGFWQLHNAELCSTAGSSRQP
                     PVKELNEYHVAGGFDEQHYALVTGNKKLINTLAQQILEAHFTESIQEEIADELGFDLQ
                     QIRKQRDPLFRKNVLRAYNYQCAICGFNMRHDDTTVALEAAHIKWKQHGGPCEIPNGL
                     ALCAIHHKAFDKGSIGLDENMRVLVSDAVNGGGSVERLFWDFDGKIIALPQVRKNYPY
                     EGFVEWHRNEVFRG"
     gene            14211..14390
                     /locus_tag="OO130_002673"
     CDS             14211..14390
                     /locus_tag="OO130_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5366022.1"
                     /translation="MQDLAASLQLAFDSGAKRVLLPISWAMDIPTVPAELFTKFQVSF
                     YSEPVDAVYKALDVN"
BASE COUNT         4095 a         3371 c         3189 g         3953 t
ORIGIN      
        1 agaagcagaa aaagcttatt atgcttccat cggaaacgat gatctggcag cctgggttca
       61 cagataaaac actctccagg aaacccgggg cggttcatac tgctcctaac gataccgtgt
      121 tattcgctgg aataatcgta ccgttgcgcg tcaactgtac gccgacgccc tgcgctggtg
      181 aaaacgacgc ggtattggtg aaaatcgagt tgcccgcatc tgcggttgtg ccggagaggt
      241 aatcccccag gttttggctt ttcgcacaat aaacggtaag aggaattggc actgaaccag
      301 ggtagtccgg cagagtaacg gtgacatcac gagcagaaac atcgcagccg ccagtaggca
      361 ccaccacatc attattggcg taaatattcc acacaaactg gaaatcatcg ctgttatagt
      421 tgttggtctg tcgcaaaata agcacggcaa ttaatgagcc agctttaatc gccaccccgc
      481 ccgcactgct cacaggcgtc aaataaagcg ccaccggcca cggcttatcc gttctcgaat
      541 tataaacaac ccgcggcgtt tcgctggtgg tcggaaatgg atagctactg ccactatatt
      601 ttacggtccc ggaaaaatta gataacacgc cgccataagc cgagcctcgt tgcagtgtga
      661 catagtctgt aatggtttcc ggataatcgt tatggcaaaa gatttgcgtc gaaagatcta
      721 cgaccaggtt ttgccccaca ttcacgacag gcgcaaggtt tacataaaca ttagcgctgc
      781 caccgccaat agggatagca gtaccattgg cggttttaca ggcgaatgac caggcattta
      841 ccgaccagcc catcagcagt acagcaaaca gggtaataac tcgtttcatt acaattatct
      901 cttcgggttc agctgtaggt ataggtgatg ctaatcactg cctgaatggt tccctgagtg
      961 gctccgccat ttaccgtcaa tgctctgacc tgtaacggga agtgcgctga ttgtgaggaa
     1021 tcatccacct gaactgtttt ggtttcgcca gtattcaatg tgttgccact gtcatcctgt
     1081 agctctaact ggatgttttg cgcggtcccc tggtttttat aatatccggt actgtcggct
     1141 gccccgctga agctggcagt gaccctcgac gttcccaccg gacaattagt caactcaagc
     1201 gcaacatcat gccaggccga tgccgccccg gcagacatca gactgaaaga ataaagatcg
     1261 ccgagatcaa ccgtggcatt ggtggtggaa actgtgcacg gtttggcgac gaccttaccg
     1321 ttcaccgtga tggtgacatc ggctgcctgt attgtcgcac ttgcgagcgc caatattgcc
     1381 gccaataaat acccacgttt gcgccatttc atgagcatct ccagttactg atattcaaga
     1441 gtgaaggtag ccgtggcatt gatatgcccc gcagtgacag gcacctgtgt cgccattagc
     1501 cgggcgtaaa aattcaacgt atttggttta cccggcgtca gggtcgtcca cgaaatcgcg
     1561 gacgatggag cattaagggg tatctgattt tgctgctcat tcagaagctg tattcccagt
     1621 cccgaagccg ctgacaccgt attttcaagt gcaagcaggt tggcattgtg gctatctgca
     1681 acgccggtaa acccaacctt tacggcagaa acggcgttac cacagggtga cagcaaaata
     1741 cgaaatggaa cgacaggagt cgttgcgcca atgttgttaa attgcttcgc cgcgttttcc
     1801 atcagatcaa cagtaaaatt ggttgattca gcggccacac tacagccgtt atctctgaca
     1861 tagccgcgga tagtaatcgt gctatccgca gccaaaacgc gacttaccgc cagccacaaa
     1921 aaagcgcaca gaagataaaa aggtttgttt ctcatcacgc ccccttaacg acattcagct
     1981 gatagctggg ttaataactg ctgctgactc tctggtggca gttgataatt ggcgatacag
     2041 tgagcatttt cctcttctcc ccatttcacc tgaacttttc ccgctaaagg cattccgctg
     2101 aggtaaacct gaccattatc cgcaacaatg ccgctactct ggctactctc tgatgtcacc
     2161 atcgccccaa acggcagcgg cttattattg tgggttagcg tcatgagcag ttttatccca
     2221 acgcgcgctt taaactctgc tcgcacgatc gccccacgag tgggaacaac gttagcgacc
     2281 gcgttatcta aatcgacgtt atcagccagg gtattggtat ccagcgccac tctgttttcc
     2341 cgatattcgg tggcataagg cagcacggca taaccgcgcc agtcggtacg cacccccgtc
     2401 tggttttcga cttttgcatc ttttgcgcca ggcgctttaa caagtaccac cgtatcgttt
     2461 aacggctgcc ccagcgttac gccattggca tgagccagta ccccaccgct gactccgtaa
     2521 tagagctgct taatatcatc gctatggctg taaccgatat tggcattgcc gtaaccaccg
     2581 cgataattca gcgtggcgta gcctgtactt ccgctattac catcgcctcc cccggcatag
     2641 ccggtttgca cgctatagct gaggttgttg tcttccagca acgtaccgta tacaccagcc
     2701 agattggtca tccgaccgtt gagatcgtgt gacacgctgt agctggcact ggcatgtcgc
     2761 cactgagatt tactgtcaga acgcagccag tggctgaaag gaatattgac gttaagcgct
     2821 aacatctgat cccgcccttt ttgccaggcg tttttcgtca ggctatagct gagcgtccag
     2881 ttgatatctt cgaacgcagt atttaatcca gcctagaatt gctcatcgac attactcgtt
     2941 ccccaataag tttgatggct accactcaaa tacagtgttg atgtgcgccc gagttgctga
     3001 gtaacggtga gctgtaattt cccgcgtttg ttataagcga ggttgtaata gtcggtgaat
     3061 ttcggcttaa cctgaataac tccatcctgt gtttcgatgt tgtagccatt cattcgactg
     3121 tatgttgtat cagcgaaatt aaaatatccg ctggtcgaat aacggtaacc cactaactga
     3181 atattcgtgc ctgactcatt gagcgatttg ttatagagaa aacgcaccga ttgtccgtca
     3241 tgctgactgt catcgggaag tgtggaatta gcctgcgtca tatccacaga cagagcgccc
     3301 agtgccccca tgtttttccc gataccgaaa ttaaaagcac gataacgatc cgccagttgc
     3361 gttccaccat atattgtcca gccagccgga aggccgtgga gtaatgtact ttggaaaaag
     3421 cggggttttt cctgctgcgt atttccacta cggtattctc ctgccgtaat ggaataacga
     3481 gtatgccctt cacgttgcaa aagcgggact gacgaatagg gtacggtaaa aatctgcgtg
     3541 ctgccgtcag cctctttgat cgttacctgc aagtcaccac tattacctgc ggcatagata
     3601 tcgttgatgg taaaaggccc cggtggcacc gtactattat aaatgtcata cccattttgt
     3661 ttaatagtga cctgtgcagt accacgagca ataccgtgga tcaccggggc aaatcctctt
     3721 tggctatcgg gtaacatatt gtcatctgag gccaattgtg cgccgcgaaa gttaatacca
     3781 tcgaaaatat cgccctgggt ataaccatca cccagcgtca gccgggaacg taacggtatt
     3841 atgtctcgct caagccaggt attgatatgc tgccatttat ttttgctacc tgatgatctg
     3901 tcgctactgt tataactcca gctggtattg tcgcgtaaac accacgcacc aatatttaac
     3961 ccactctgta ggtttaaata tgcataatgg ctgttacccc caatctgatt ctgtacacta
     4021 tttccgctga aattataatt gagcaatccg gcattaatac cgggatccca taactcagga
     4081 ggaatataac cacgcgcgcg attactcata aatgcctgag ggatcgtcag gttcagtcgc
     4141 tgctgaccaa catccagatg cgcagtagcg tcatggatca ttgaggttaa tggcacgcag
     4201 gcatcatctg ccagcagatt cataccggag acagaagccg cattcagccc catactggca
     4261 agttgcgcgc gtgtcaggca gggaacaatc ccttgttcac tgtcgcccgt attaaatgtg
     4321 acatcacgcg ttgccatata accattattc aaatagatat cgacgcgata cgtccctggc
     4381 ggtaattctt gcccattttc aaaacgcgat aaatcggcca cagcctgggg atcatccgct
     4441 aaaaagcgcg gattaaaata gagttcggca gatgacaaag gtgcctgtgc ggcaaaagca
     4501 caggcgacaa cgagccggac aaaaaaacca gccaaacgat gcttacgaat atgcaagcat
     4561 tgtgtgtttc gctggtaaag tcttaaattc agatatgaca ttattcctcc ggtttaaaat
     4621 tacccggcag tcaattcttt ttattcaggc gagaaattcc ccctgcgtta ttccattacg
     4681 cccgtcattt tgggggtaag tgcgccataa tcatttattg ttcggtaagt aatattgctt
     4741 cctgcatcag aaggcaattt aaccgtgctt tcgcccattg gaggcaccaa tgcattttca
     4801 agaacccggg ttccggcatt caactctgtt accgtcagat aatagggtgt cgggttaatc
     4861 agcgtcagag aattcgcgct acgacgaaat cttaattttt ctgcggcctg atcgggtggc
     4921 aacgctaatt tagccgggcg atagtacagt ttaatgcggc tgataattgc gaactgtagc
     4981 gtattctcag tcaattttga tttatccatt gacggaatcg ctttaacatt catccagaat
     5041 aaactttccc ggtcctgtgg caattggtta tttgttgcat caagaatacg caaggtattc
     5101 tctttttttc ccttcatcgc aaacagagga ggcgtcacga taaaacgacc atcctttaca
     5161 ccatcggcat tttccatcca tgattgaatt aaataggtac tattttcatc attatttgtc
     5221 acggcaagtt gcacttgttt ttgccctgcc ggataaatta cgcgagtcgc acctaaggcc
     5281 actcccgctt cagcgcgtcc ggcaaccatc attgccatga acatcaggat acctgccagc
     5341 aagcagaatg ttatttcctg cgatttcctt acattggcgt ttttattact cactgtcctg
     5401 ttcctgttat cacattatct gctgaacaat tactgatagg ttaaagagaa ccaggcctgg
     5461 gcattggcga tgccgccagt aacccaacgc ccggtagcac gatatttggc gatgaaatgt
     5521 agcgaagtag agcctgagta aagccgtttc cagtttactg gaggacgatt aatcggtacg
     5581 aggtttcctt catcatcaaa caacgctacg ccaatattgg tggctatccc tggcccctct
     5641 cccacggaaa gcacatccgg atttttacca tccgcgacac cgtgaaacgc cacacccaca
     5701 cgttcactca ccaccgtgct acattcccgt aaatgaataa caaaaggcac cggtgcgcta
     5761 tcttccccta ccgcatgaaa ccggttactg ctgatttgcc ccatattgac cgtcatttgt
     5821 ttatcaccgg cttcaatccg gcaagtttcc gcaataatga cgccctgaaa ttgcatattt
     5881 ccgccgggca gcgtagtatt ccatttattt ccggccagag caaacattgg cagcaacgac
     5941 gctaatagaa ataatctctt ccttttcatc gttattttta tcgcacaagg gtgggcatcc
     6001 ctgcccgtaa tgacgtccct gaacctgggt aggttattga tactgaacct tgaaggtcgc
     6061 atccgcatta gctgcgccag gggttgcgac accggttgca taataacgcg cctggaacgg
     6121 aatggtgtta gtaccgttat tcagggttgt ttgctcactg aatgtcgcac catccagcgt
     6181 cagcgcagca cccgttctgt ccaggatctg tacaccaacg tttgttgcgc tacccgcagc
     6241 tgaactctgc agagccagaa cgttggtatg acccgcatca atcgccgtac ctaaaaaggc
     6301 aactgcggct ttagatgcaa cattggtatc gcaatcattc agctgaatgt taaaaccgac
     6361 agcagaactg gttgctcctt cctgtgccag cgatgcggta cgaacctgtc ctaactgaac
     6421 gatttgatca acagagcctg catcaactgc gcaagcggcg ttaacaactt cccctttaaa
     6481 gtgaacggtc ccaccattaa ccgtcgtggc agcggccaga gccgctgtag aactgaggga
     6541 cagagccgac agaacaacga ttgccagagt tttaattttc atgctgcttt cctttaaaaa
     6601 aactatttct aaatcgacat gggcagtcgt tctgtacact ttgttttgtc aacgagtttg
     6661 actgccaaca ctgcacagtt ttcccccaaa agatgaaaca tttggggcca ttttgactca
     6721 tagaggaaag catcgcgcac aaactttttc agtttatttg ttggcttaat attctattgt
     6781 tatctttatt tatagatttt tatattgcat gaggtggttt ttggagagaa gaatgaggaa
     6841 gatgcgtcga gccacagaaa cgttagcttt acatatagcg gaggtgatgt gaaattaatt
     6901 tacaagagaa ataatttaca tatcaaacag ttagatgatt tttgtcgttt tttaaaattt
     6961 ttatgcttga gaaaaaatac gtaacttatt tatgatatgg acaatttggc cccaattgtc
     7021 ttgtatttat ttatttttta actttattat caataagtta attcaaacct cttctctttt
     7081 taatttttcg tttatgagat tatttctttc ccataatccg gcaaaacgag cagcattact
     7141 ggcggtataa cgtacagtat ggcgaatatt tcgatgcccg agataatcct gaattaaacg
     7201 agtatctgca ccacgctccg ccagttcata accgcaagca tgccttaaca tatgaggatg
     7261 agtctgcgtt acggttccag cttcaatacc ggcatcacga ataatgcgat aggcctgctg
     7321 gcgagaaagc cgactcccgc ggcgagaaat aaatatggcg tcggtccggt cagcgccttt
     7381 ccagttagca cgttccaggg tccagcgttc cacagcttca cgctcatcaa aacgtaacgg
     7441 gtgaacggta gaaaatccgt tcttcagtcg gcgaatattt attcgacctt cattaaggtc
     7501 aagatcctga taatgcagat caagcagttc actaatacgc atcccatgcc gatatgccaa
     7561 cagaataaga caataatctc tggctcccgt tgccccgtaa caaaccgcct gcatcatggc
     7621 ctgaacttct ttaccggtaa gataacgacg tttactcaca atagtagtac tcctaactga
     7681 gatatatttg aatgaatacc tataggaaac ctcaatcggt caaaattagc ccaatagata
     7741 aaaagataac cacacacgaa gtgatgtggc tatcagtcaa ttacattaaa tatcatacaa
     7801 aaataaaata tcactgatgt gacaacatct attttcattg atttgattca atataacaat
     7861 agattgtagt agattagctt ccagttgctt aaaaaataaa gaaaaaagtg cgaaaacagg
     7921 agaatttaag tgatgtgtaa atttatgaat gattttgttt atttattttg aagaacataa
     7981 accattgtat tttgtatgga tatggttaac ttaatgctta ttatgataat taagaattat
     8041 acttacaata acatcaatta ataaatggtt aatcattttt gatatatcgt aagaataatg
     8101 tagtttttaa caccatccct ggtatctcaa ctatctctat aaaacagcgt gacgctgtcg
     8161 tcctctggct ctatcccaga tgccgtaaaa acgccctgca ttgctggcgg tataccagac
     8221 agtatgacga atattgcgat gcccaagata atcctggata agtcgcgtat ctattcccat
     8281 attcgccaaa gcaaaaccac acgaatggcg taacatgtgc ggatgaatct ccagtgacaa
     8341 cccggcatta ccaccggaag tcgagataat atggtaaaac tgttgccgag aaagcggatt
     8401 ccccttacgc gataaaaata cccactcgct ctcagcatgc gggtacgaag tacggatact
     8461 caaccagttt tttaaagcct gaacttcttt attcaatagc gggggcgttg ttgaaaagcc
     8521 tttttttaat cgatggatat atatacactt tgccttaaga tcaatatccg aaatcctcaa
     8581 tcgacaaatt tcactcgccc ggaaaccatg aataaaacaa agcaaagtca gacaataatt
     8641 acgtgctgca tgaggcccgg tatttgctgc tttaaggagt gattcgattt cactatgggt
     8701 caggaagttc ctttttttgt tatcagcctt attcttcatc gtttttccct tataattaca
     8761 gacgcgcact agctgtgctg ggttagcaat aatccaacat ttttatcctg attatgttta
     8821 taaaagcgaa cgtttgctta ataactaacg taagtggacc agttcttctg agtgaactta
     8881 aatggggtag caacagttaa ttataataac gttgccatac aaacgctatg ctcttgcatg
     8941 ctatgtacct ttatatattt atcaatcgga tacgaatcta ataatacccc tcatcattaa
     9001 tgcaactaat cttatctata tatcatgtga tatgttctgt aacaagtaaa atcagcataa
     9061 aaaattcagc aaaatttaat attcgttcaa ctagccgtct cacatatgac attattagtc
     9121 aactccattt gccacaaaat ggctatttca tggaacgcag taatttctta gtggctatta
     9181 tcatgctaat tcttaggtcc ctagcgatta ttcctggctg attcgtcagc ttacgccatg
     9241 agattgttac attccttttt atcctggaga tatgggggaa cgcaggagat gattttttca
     9301 tctacattcg ttggtaaata tagctttggt cactacgact taaaccttcc tcaatcacag
     9361 ccattcgccg ggcgatacgg caaacaaact aagtgaaaca tcatcctgaa aacaccaaca
     9421 tcaacaagcc tctccagatc gacttcagaa gtgaccagtt acaagccaca aaccaggaaa
     9481 catatttcat gatgagaatt atgctcagcc tgatggcggg taaacactga ttttcaccaa
     9541 gctcactgag ttagcttgca aagcttccct tgtttagact ccaaataaat tatttataaa
     9601 acaaacattt aaacaaagat ctttagtttt tgaagaccac cgcaagtgtt cgtctggctt
     9661 cacatggcat cttcctctta gaaaaagatc gacatatttt gtgacacgaa ttgcaaatct
     9721 ggttttgttg tatggattgc gtgatttttg atctggtata acaggtataa aggtgcacca
     9781 agatagtcaa tgagacaggg catctcgcaa tctatggcaa acatcacttc agttctttct
     9841 catcgggtga tgaaaacgca cttcagtctg aaaggaatat gaaaatgaga tcaacagaca
     9901 ctctatttta tgactctggg taaaatggat tgagtaagtg atatagctta cgaaaattca
     9961 aatcaattaa acatcagaag agattttata ctcaggtatt taatctggat ctctgtttat
    10021 ttaaataatg tgaaaagaga tttttcacag gagaccttat acaaaaaaat ataaaataca
    10081 gctaccggtt gccaaagaca ctataagcct ggcaaaaaat attacacaac ataaatgcta
    10141 attgtttatg cgggctttgt attgctttct gtatcctaca aatgagtgaa atttatgaaa
    10201 aaggctaaaa tactttctgg cgtattatta ctgtgctttt cgtccccatt aatttctcag
    10261 gctgcgacac tggacgtacg tggtggatat cgtagtggaa gccacgccta tgagactcga
    10321 ctcaaagtca gtgagggatg gcaaaatgga tggtgggcaa gcatggaaag taatacctgg
    10381 aataccattc atgataataa aaaggaaaat gccgcactca atgatgttca ggttgaagtt
    10441 aattacgcga ttaaacttga tgatcaatgg acggtgcgcc cgggaatgtt aacgcatttt
    10501 agcagcaacg gcacacgcta cggaccctac gtaaaactgt cctgggacgc gacaaaagat
    10561 cttaattttg gcattcgcta tcgttacgac tggaaagctt accgacaaca agacttatcc
    10621 agtgatatgt ctcgtgataa cgttcatcgt tgggatggat atgtcactta ccatattaat
    10681 agtgatttca ccttcgcatg gcaaacgacg ctatacagca aacagaacga ttatcgctat
    10741 gcaaaccata agaaatgggc gacggaaaat gcatttgttc tacaatacca tatgacgccc
    10801 gatattacgc catacataga atatgactac cttgaccgtc agggtgttta caacggcaga
    10861 gataatttat cggaaaacag ttatcgcatt ggtgtgtcat ttaaactgta gtagacagga
    10921 gacagtcaca atgaataaaa caataacggc gcttgctatc atgatggctt catttgccgc
    10981 aaacgcgtct gtattaccgg aaactcctgt gccatttaaa agtggtaccg gagcaattga
    11041 taacgacact gtctacattg gtttaggtag cgcaggtacg gcatggtaca agctggatac
    11101 acaggccaaa gataaaaaat ggacagcgtt agctgcattc cctggtggac caagagatca
    11161 agcaacctct gcatttattg atggcaatct gtatgtgttt ggcggcattg gcaaaaacag
    11221 agagggcttg actcaggtat ttaatgacgt acacaaatac aaccccaaaa ccaatagctg
    11281 ggttaaattg atgtcgcacg caccgatggg catggcgggt catgtgactt ttgtacacaa
    11341 cggcaaggct tatgttactg gcggtgttaa ccagaatatc ttcaatggct attttgaaga
    11401 tctcaacgag gctggaaaag attcaaccgc tgtagataaa atcaatgcct actattttga
    11461 caaaaaagca gaagattatt tcttcaataa gtttctgttg tcttttgatc cctcaacaca
    11521 gcaatggagt tacgctggcg aatcgccctg gtacggaacg gctggtgcgg cggttgtgaa
    11581 taaaggtgat aaaacctggc ttattaatgg cgaagccaaa cccggattgc gaacggatgc
    11641 cgtatttgaa cttgatttca ccggtaataa tttaaaatgg aataagcttg ctcccgtctc
    11701 atcaccagat ggcgtcgctg gcggttttgc ggggataagc aatgattctc ttatatttgc
    11761 cggaggggcc ggattcaaag gttcacgaga aaattaccag cacggaaaga actatgcgca
    11821 tgaaggcctg aaaaaatcat atagcactga tattcatctt tggcataacg ggaaatggga
    11881 taaatcgggt gaattatcgc aaggtcgggc ctacggagta tcattgccct ggaataatag
    11941 tctattgatt attggcggtg aaactgcagg cggcaaagcg gtgacggatt cagttttgat
    12001 ctctgtgaag gataataaag tcacagtaca aaactaacgc ttcagggccc cagtaaaggg
    12061 gccctgctat caatttatca tttcaattac gcgaaaatta tatggacgca ataatatcgc
    12121 ccgattatta ctatgttctt accattgctg gtcagtctaa tgctatggcg tatggcgaag
    12181 gactgccatt accggccagg gaagatgcgc ctcatcccag aattaaacaa ttagcgagat
    12241 ttgcgcatac gcatcccgga ggcccgtcat gtcactttaa cgacattatt ccactaactc
    12301 actgcccaca cgatgttcag gatatgcagg gttatcacca tcctctggca acgaatcatc
    12361 aaacacagta cggcaccgtt ggccaggcac tgcatattgc acggaaatta ctgtccttta
    12421 ttcctgataa cgcaggggtt ctcatcgttc cgtgttgccg tggcggatcg gcttttaccg
    12481 cgggcagcga agggacatat tcagaacggc acggagccag ccatgatgct tgtcgttggg
    12541 gaacggatac tccgctatac caggatttag tcagcagaac gcgagccgca ctggcaaaaa
    12601 atccgcagaa caaattcctc ggcgtatgct ggatgcaagg cgaatttgac ttaatgacca
    12661 gtgactacgc gtcacaccct caacacttta atcatatggt tgaagccttt cgtagggatc
    12721 taaaacaata ccattctcag cttaataata ttactgacgc gccgtggttt tgcggcgata
    12781 ccacctggta ctggaaagaa aatttccctc atgcgtatga agctatttat ggcaattatc
    12841 aaaataatgt tttagctaat attattttcg tcgacttcca gcaacaaggt gaaagaggac
    12901 tgacgaacgc gcctgatgaa gacccggacg atttaatcat gggatattac ggttcagcgt
    12961 accgctcacc ggagaactgg acgacggcac tgcgaagcag tcatttcagc gcggcagccc
    13021 gtcgggggat tatttctgac aggtttgtag aagcaatttt gcagttttgg cgcgaaaagt
    13081 gagcgttcat taatttgatt tattcgcatc catcaacgct tttaactcct ccggcgtaaa
    13141 cggtggtttg cgtcggtgca acatcgcatg gcaattaggg cacacaagca gcaaaaaaac
    13201 aaatgagatc aaggtaatat atcttcactt gcaggcttga acagcagaaa atttgcgagc
    13261 atgatcgcaa ttatttcact gctagggata tcaatggctt ccagtaaatc gctacagcag
    13321 gcaattgcca atataaaaat ctggcataag ggtgaacagc gcgcaccgca taaaccattg
    13381 ttattgctat acgtattagc gggatacctg aatggacatc cacgcctttt cgattatgga
    13441 tcggaaatct acgaacctct gcacagttta ctggaacgtt ttgggccaca gcgttcacag
    13501 taccgacctg atatgccgtt ctggcgatta caaggcgacg gattctggca attacacaat
    13561 gccgagcttt gctcaacagc agggagcagc cgacaaccgc cagtgaagga actgaacgaa
    13621 taccacgttg caggcggttt cgatgaacag cactacgccc tggtaaccgg caataaaaaa
    13681 ctcatcaata ctctagcgca acagatactc gaagcgcact tcaccgagag tattcaggaa
    13741 gaaattgctg acgagctggg ttttgatctc cagcaaatcc gtaaacagcg cgacccgtta
    13801 tttcgcaaaa atgtattacg cgcttataac taccagtgcg cgatatgtgg ctttaatatg
    13861 cgccacgacg acaccaccgt cgcgctggaa gcggcgcata taaaatggaa acagcacggc
    13921 ggtccttgtg aaattcctaa tggcctggcg ctttgcgcaa ttcaccataa agcctttgat
    13981 aaaggatcga ttggcctgga cgaaaatatg cgggtactgg tatcagatgc agtaaacggc
    14041 ggagggagcg ttgagagatt attctgggat tttgacggga aaattattgc gttgccgcag
    14101 gtgcgtaaaa attaccctta tgaggggttt gtggagtggc atcggaatga ggtttttagg
    14161 gggtaaggtt gataactatc gcagtatgtt caatattgct gacataatca gtgcaggatc
    14221 ttgccgctag tttacaactc gccttcgata gcggcgcaaa acgagttctg ttgccgatat
    14281 cctgggctat ggatattcca acagttccgg cagagttgtt taccaagttt caggtaagtt
    14341 tttactcaga accggttgat gcggtttata aggcgctgga tgtgaattaa cacattcctc
    14401 agcttattta caaaaggatt attcccattt gatataatat cagtgccaat ttactttttt
    14461 caatggaata tatctcggct aaataacaat aaaacttatc gctaatggat taaataaaca
    14521 attttctaat tcatttcacg gtaatgactc caacttactg atagtgtttt atgttcagat
    14581 aatgcccgat gaccttgtca tgcagctc
//
LOCUS       DAJTMC010000098        14564 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.098, whole genome shotgun sequence.
ACCESSION   DAJTMC010000098 DAJTMC010000000
VERSION     DAJTMC010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14564
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.098"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            <1..420
                     /locus_tag="OO130_002674"
     CDS             <1..420
                     /locus_tag="OO130_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCR5366023.1"
                     /translation="LMNNSDARKTSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRL
                     AEFGGLRHKAYRLGGDEFAMVLYDVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTL
                     SIGYAMTIEHASAEKLQELADHNMYQAKHQRAEKLVR"
     gene            436..918
                     /locus_tag="OO130_002675"
     CDS             436..918
                     /locus_tag="OO130_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HCR5366024.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(995..1342)
                     /gene="rplS"
                     /locus_tag="OO130_002676"
     CDS             complement(995..1342)
                     /gene="rplS"
                     /locus_tag="OO130_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HCR5366025.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(1384..2151)
                     /gene="trmD"
                     /locus_tag="OO130_002677"
     CDS             complement(1384..2151)
                     /gene="trmD"
                     /locus_tag="OO130_002677"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HCR5366026.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(2182..2730)
                     /gene="rimM"
                     /locus_tag="OO130_002678"
     CDS             complement(2182..2730)
                     /gene="rimM"
                     /locus_tag="OO130_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HCR5366027.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEDYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(2749..2997)
                     /gene="rpsP"
                     /locus_tag="OO130_002679"
     CDS             complement(2749..2997)
                     /gene="rpsP"
                     /locus_tag="OO130_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HCR5366028.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(3134..4495)
                     /gene="ffh"
                     /locus_tag="OO130_002680"
     CDS             complement(3134..4495)
                     /gene="ffh"
                     /locus_tag="OO130_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HCR5366029.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            4662..5453
                     /locus_tag="OO130_002681"
     CDS             4662..5453
                     /locus_tag="OO130_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HCR5366030.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            5474..6760
                     /locus_tag="OO130_002682"
     CDS             5474..6760
                     /locus_tag="OO130_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCR5366031.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(6815..7408)
                     /gene="grpE"
                     /locus_tag="OO130_002683"
     CDS             complement(6815..7408)
                     /gene="grpE"
                     /locus_tag="OO130_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HCR5366032.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            7531..8409
                     /gene="nadK"
                     /locus_tag="OO130_002684"
     CDS             7531..8409
                     /gene="nadK"
                     /locus_tag="OO130_002684"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HCR5366033.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCKKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            8495..10156
                     /gene="recN"
                     /locus_tag="OO130_002685"
     CDS             8495..10156
                     /gene="recN"
                     /locus_tag="OO130_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HCR5366034.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMAYGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALEIARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALATQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLNKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            10305..10646
                     /gene="bamE"
                     /locus_tag="OO130_002686"
     CDS             10305..10646
                     /gene="bamE"
                     /locus_tag="OO130_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HCR5366035.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(10708..10998)
                     /locus_tag="OO130_002687"
     CDS             complement(10708..10998)
                     /locus_tag="OO130_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HCR5366036.1"
                     /translation="MPGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(10988..11425)
                     /gene="ratA"
                     /locus_tag="OO130_002688"
     CDS             complement(10988..11425)
                     /gene="ratA"
                     /locus_tag="OO130_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HCR5366037.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELASNMVQAFTVRAKEVYSAR"
     gene            11596..12078
                     /gene="smpB"
                     /locus_tag="OO130_002689"
     CDS             11596..12078
                     /gene="smpB"
                     /locus_tag="OO130_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HCR5366038.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            12293..12655
                     /gene="ssrA"
                     /locus_tag="OO130_002690"
     tmRNA           12293..12655
                     /gene="ssrA"
                     /locus_tag="OO130_002690"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(12816..14564)
                     /locus_tag="OO130_002691"
                     /pseudo
     CDS             complement(12816..14564)
                     /locus_tag="OO130_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417134.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
BASE COUNT         3592 a         3911 c         3431 g         3630 t
ORIGIN      
        1 ttgatgaaca attccgatgc ccgaaaaacg tcggcgttac tatttcttga tggcgataat
       61 ttcaaatata tcaatgatac ctggggtcat gcgacgggcg atagggtctt gattgaaatc
      121 gcaaaacggt tagctgaatt tggcgggctg cgacataaag cataccgcct gggcggcgat
      181 gaattcgcta tggtgctcta tgatgtacag tcggaatctg aagtgcaaca gatatgctca
      241 gcactgacac aaatctttaa tctcccgttt gatcttcata atggccatca gaccaccatg
      301 acattaagca ttggttacgc gatgaccatt gagcacgcct ctgcggaaaa attacaagag
      361 cttgccgatc acaatatgta tcaggccaaa caccagcgtg ccgaaaagct ggtgagataa
      421 caaggatata tcgatatgat aaagcacctg gtagcccccc tgattttcac ctcactaata
      481 ctgactggct gccagtcccc tcagggaaag tttactcctg agcaagtcgc cgctatgcaa
      541 tcttatggat ttactgaatc cgccggcgac tggtcgctgg gcttatcaga tgccattctg
      601 ttcgcaaaaa atgactacaa attgctcccg gaaagccagc aacagatcca aaccatggca
      661 gccaaattgg cctcgacagg gctaacacat gcccgtatgg atggacacac cgataactat
      721 ggtgaagaca gttacaacga aggcttatca ttgaaacggg cgaatgtcgt ggccgatgca
      781 tgggctattg gtggacaaat tccacgcagc aatctcacca cacagggttt aggaaaaaaa
      841 tatcccatag ccagtaacaa gaccgcccag ggccgcgccg agaaccgccg cgtcgcagtg
      901 gtgattacaa ccccttaaaa cacgagcaaa cagacaaaaa aagggccagc caattggcca
      961 gcccttctta acaggatgtc gcttaagcga aatcttagtt aagacgctct ttgatacgag
     1021 cagccttacc agtacgctca cgcaggtagt acagtttagc tttacgaaca gcaccacgac
     1081 gtttgacaga aatgctgtca actaccggag agtgagtctg gaagacacgc tcaacgcctt
     1141 cgccgttgga aattttacga acagtgaatg cagagtgcag accgcggtta cgaatagcga
     1201 taaccacgcc ctcgaatgcc tgcagacgtt ttttggaacc ttcaacaacc catactttca
     1261 cttccacggt atcacccgga cggaaggaag gtacgtcctg cttcatctgc tcttgttcaa
     1321 gttgcttaat aatgttgctc ataatttaat ctcttatcct gggtaaactg atatctcggg
     1381 ggcttacgcc atcccatcat gtttatgttg ctgttgtgcg tgttccgttt tgaactccgc
     1441 cagcaacctt gcttgctctt cagtcagagc caggttttcc agaagttcag gtcttctaag
     1501 ccaggtacgg cccagcgact gtttcaaacg ccagcgacgt atctcggcat ggttgcccga
     1561 cagtaacact ggcggaactt ccatcccttc taacacctca ggccgcgtat agtgcgggca
     1621 atccagcaat ccttcagcaa aggaatcttc cgttgccgag gcttcatgtc ccagtacccc
     1681 cggaataaac cgggaaacgg agtcaatcag cgtcattgct ggtaactcac caccactgag
     1741 aacgtaatcg ccgattgacc attcttcgtc aatttcggtt tggatcacgc gctcatctat
     1801 accttcgtag cgaccgcaca ccagaatcaa tttttgattc gttgccaatt cgctgacgcc
     1861 cgcttgatca agcttgcgtc cctgtggtga cagataaatc acctttgcgc cttcacccgc
     1921 cgcggctttt gctgcatgaa tggcgtcccg caagggttgc accatcatta acatccccgg
     1981 tccgccgccg taaggacgat cgtccacggt acggtgccgg tcatgcgtga agtcgcgagg
     2041 actccagctc tggatgctca gcaggccatt tttaactgcc cggccagtta ccccgtaatc
     2101 ggtaattgcg cggaacattt caggaaacag gctaattatg ccaatccaca tagcgccgtc
     2161 ttttaccgtt tatccggtgg tttaaaaacc aggatcccaa tctacttcga ttgaacgagt
     2221 agtgagatcg actttcttga taacctgccc atcgaggaac ggtacgagac gttccttgat
     2281 accaaacgca tctttcaggt ttgccttaat gacgagaacg tcattagatc cggtttccat
     2341 catatcgacg actttaccga gatcgtagtc ttcagtggtt actacctggc agcccatcag
     2401 gtctttccag tagtagtcac cctcttcaag ctgaggcaac tgcgatgaat ccacgacaat
     2461 ttcacaattc gtcagcaggt tcgccgcatc acgatcgtca acgcctttca gcttgatgat
     2521 catgtcctga ttgtggtgct tccagctttc cagctggact tgctgccact gacccgcctt
     2581 ctggataaac cagggctgat agtcaaaaat gctttcggcg tcttcggtgg aagaaaacac
     2641 tctgagccac ccacgaatac cgtaagacga acccattttt cccaaaacga tgggatcaac
     2701 aggtgcttgc gcggtgagtt gtttgctcat catgaccacc gtgacagatt aagctgcttt
     2761 gtttacttct ttgatcagcg cagcaacgcg atcagaaata gttgcgccct ggccaaccca
     2821 gtgagcgatg cgatccagat ccaggcgagt gccttcttct ttttcgctag cgattgggtt
     2881 gaagaaacca acgcgctcga tgaagcgacc gttgcgtgca ttacggctgt cagcgacaac
     2941 aacctggtag aacggacgct ttttagcgcc gtgacgtgct aaacgaatag ttaccataac
     3001 atcctcttgt gtgaataaaa caaccggacc ccatcgagga acggagtccg gtgtcatatt
     3061 aaaagcccga aaattttact gatttctgga gaaaatgcaa tcagcagtta ataactctgt
     3121 cgcataaggt catttagcga ccaggaaatc ccggcggcat catccccttc atgcttctca
     3181 tcatcttcgc cattccgccc ttcttcattt tcttcatcat gcgctgcatg tcgtcgaact
     3241 gtttcagaag acggttaacg tcctgcacct gcataccgca accggcagca atacggcgtt
     3301 tacgcgaacc tttgatgatt tctggcttag cgcgctcttt catcgtcatc gagttgatga
     3361 tggcttccat acgcaccagc actttatcgt ccatctgtga cttgacgtta tccgggatct
     3421 gccccatgcc cggcagcttg cccatcaggc tggccatgcc gcccatattt ttcatctggc
     3481 gcagctgctc aagaaagtcg ttgagatcga agccgtcacc ttttttcagc ttgctggcta
     3541 atttctctgc ctgcgcgcgg tcaactttgc tttcgatatc ttcgatcagc gacagtacgt
     3601 cgcccatgcc gagaatacgc gacgcgatgc ggtccggatg gaacggctcc agcgcctcag
     3661 ttttctcgcc aacaccgagg aacttgatcg gtttgccagt gatgtgacga atagagagcg
     3721 ccgcaccgcc gcgggcatcg ccgtccactt tggtcaacac tacgccggta agcggtaacg
     3781 cttcattgaa tgcttttgcc gtattggccg catcctgacc ggtcatggcg tcaaccacaa
     3841 acagggtttc aaccgggtta atcgacgcat ggacttgttt gatctcgtcc atcatcgctt
     3901 cgtcaacgtg cagacgacca gcggtatcca ccagcagcac gtcgtagaat ttcagtttgg
     3961 cttctttcag cgccgcgtta acgatatcta ccggcttctg accaacatca gaagggaaga
     4021 aatcaacgcc cacctgctct gccagcgtct caagctgttt gattgccgcc gggcgataaa
     4081 cgtcggcaga aaccaccagc actttcttct tgtgcttctc gcgcaggaac ttaccgagtt
     4141 taccaacgct ggttgtttta ccggcacctt gcaggcccgc catcagcacg accgcaggcg
     4201 gttgcgccgc caggttcagg gtctggttct cttcgcccat cgccgcaacc agttcgttac
     4261 ggacgatttt gacgaactcc tgccccggcg tcaggctctt attaacttca tgaccaaccg
     4321 ctttctcttt tacgcgattg ataaactcac gcactaccgg cagagcgaca tccgcctcca
     4381 gcagcgccat gcgcacttcg cgcagcgtat cttttacgtt gtcttcagtt aggcgtccac
     4441 ggccactgat attgcgcagc gtgcgcgaca aacgatcggt taaattatca aacattgtct
     4501 ctcgcctggg gtggaaacgg ttggccgcaa tcgcgacaca tcatcagtat tttgccgcag
     4561 tataacatga aggcgtcttt gttgttatgc aacggttgga gcagcgttca cctgacgcta
     4621 tactgcttct ctttcttatt gctcaaactg ttgacatcac tatgcccgtt tttgctctgc
     4681 tcgcgcttgt cgcctactcc gtcagtcttg cgctgattgt tcccggtctg ctgcaaaaaa
     4741 acggcggctg gcggcgcatg gctattattt ctgcggtcat tgcgctggtc tgccacgcaa
     4801 tcgctctgga agcccgcatc ctgcccgacg gtgatagcgg acaaaacctc agcctgctga
     4861 acgttggttc attggtcagt ttgatgatct gtacggtaat gaccattgtg gcttctcgca
     4921 atcgtggctg gttgctgcta cccattgtct atgcctttgc gcttatcaac ctggcgctgg
     4981 cgaccttcat gcccaatgaa tacatcaccc atctggaagc aacgcctggg atgctggtgc
     5041 acattggctt atcgctcttt tcctatgcca cgctaattat cgccgccctg tacgcgctgc
     5101 aactggcgtg gattgattac caactgaaga acaagaagct ggcatttaac caggaaatgc
     5161 cgccattgat gagtatcgag cgtaaaatgt tccacatcac gcagattggc gtggtgctgc
     5221 taacgctcac gctttgcact ggcctgttct acatgcacaa cctatttagc atggaaaata
     5281 tcgacaaggc tgtgctctct atcgtggcgt ggtttgtcta tattgtgctg ctgtggggac
     5341 attaccatga aggatggcgt ggacgccgcg tcgtctggtt taacgttgcg ggcgcggtaa
     5401 ttctgacact ggcctacttc ggcagccgaa ttgtccagca gttaatcagc taaacccaga
     5461 aaaggagttt cccctggaac acatttctac tactacgttg atcattattc tgatcatcat
     5521 ggtggtcatt tcagcctatt tttccgggtc cgaaaccgga atgatgaccc tcaaccgcta
     5581 tcgtctgcga catatggcga aacagggtaa tcgctcggcc aaacgcgtcg aaaaattgct
     5641 gcgtaagcca gaccgcctga taagcctggt gttaatcggc aataacctgg tcaatattct
     5701 tgcctccgcg ctcggcacta ttgttgggat gcgtttgtac ggcgatgcgg gcgtggcaat
     5761 tgcgactggt gtgctgactt ttgtcgtact ggtatttgct gaggtattgc cgaaaaccat
     5821 tgccgcgctg tacccggaaa aagtcgctta tccgagtagt tttctgctgg ctccgctgca
     5881 aattttgatg atgccgctgg tctggttgct gaatgctatc acccgtatgc tgatgcgcat
     5941 gatgggtatc aaaaccgata tcgtggttag cggctctttg agcaaagaag agttgcgcac
     6001 tatcgtgcac gaatcacgct cacaaatttc ccgtcgcaat caggatatgc tgctgtcggt
     6061 gctcgatctg gaaaaaatga ccgttgatga catcatggtg ccgcgcagtg aaattatcgg
     6121 tattgatatc aacgatgact ggaaatcgat tctgcgccaa ctctcccact cacctcacgg
     6181 gcgcatcgtg ctctaccgtg attcgctgga cgacgccatc agtatgctgc gagtacgtga
     6241 agcctggcga ttgatgtcgg agaaaaaaga gttcaccaaa gaaaccatgc tgcgcgccgc
     6301 ggacgagatc tattttgtgc cggaaggtac gccgctcagc acgcagttgg taaagtttca
     6361 gcgcaacaaa aagaaagtcg gcctggtcgt caacgagtat ggagacattc aggggctggt
     6421 gacggttgaa gatattctgg aagagattgt cggcgatttc actacgtcga tgtcgccaac
     6481 acttgccgaa gaggtcacgc cgcaaaacga cggttcggtg attatcgatg gcaccgccaa
     6541 cgtgcgggaa atcaacaaag cctttaactg gcatctaccg gaagatgatg cccgcacggt
     6601 taatggcgtc attcttgagg cactggaaga gatccctgtc gcaggcaccc gcgtgcgtat
     6661 tggcgagtac gatatagata ttctcgacgt acaggacaat atgattaagc aggtaaaagt
     6721 ttttcctgtg aaaccgctgc gcgagagtgt ggcggagtaa cgaaaacggc cctgcattcg
     6781 catgcagggc cgtgaattat tacgaaaaca gaaattacgc ttttgctttc gctacagtta
     6841 ccatcgccgc acgaatcgta cgaccattca gcgtataacc cttctgcata atgcccagta
     6901 cgttacctgg cgcaacgtca tcagattcca ccattgcgat agcctgatgc acattcgggt
     6961 ccagcggcac gttagtttcg gcgatcactt caacaccaaa cttacgcaca acatccagca
     7021 tcgacttcag cgtcagctca atgccttcaa ccatcgcaga catatccggg ttagctttat
     7081 cagccacttc cagcgcacga tccaggctat caatcaccgg cagcaattcg ttgatgaatt
     7141 tctccagcgc aaatttgtgg gctttttcaa tatccagttc agtacgtcga cgcaggtttt
     7201 ccatttcggc ttttacacgc aaaatgccgt cacgttcacg ggtctgggct tcagccagct
     7261 gagcttcgag attcgcaatt ttttcatcgc gcggatccac ctgctcagca gaagcttctg
     7321 gctcaactgc ctcaatctct tcgtgctgat ccatgataat ttcttccggg gcttgcccct
     7381 caggcgtttt ctgttcttta ctactcatga atttctccgc gtttttttcg cattcatctc
     7441 gctaacttcg cttattatgg ggatcagttt cagggtttca agggaagcac tcacattgtc
     7501 atcaatcttc gcaacaagga cctcggaaaa atgaataatc atttcaagtg tattggcatt
     7561 gtgggacacc cacggcaccc cactgcactg acaacacatg aaatgctcta ccgctggctg
     7621 tgcaaaaaag gttacgaggt catcgttgag caacaaatcg ctcacgaact gcaactgaag
     7681 aatgtgaaaa ctggcacgct cgcggagatt gggcaactag ctgatctcgc ggtagtcgtt
     7741 ggtggcgacg gtaatatgct gggcgcggca cgcacactcg cccgttacga tattaaagtt
     7801 attggaatca accgtggcaa cctgggtttc ctgactgacc ttgaccccga taacgcccag
     7861 caacagttag ccgatgtact ggaaggccac tacatcagcg agaaacgttt tttgctggaa
     7921 gcgcaagtct gtcagcaaga ttgccagaaa cgcatcagca ccgcgataaa tgaagtggtg
     7981 cttcatccag gcaaagtggc acatatgatt gagttcgaag tgtatatcga cgagatcttt
     8041 gcgttttctc agcgatctga tggactaatt atttcgacgc caacaggctc caccgcctat
     8101 tccctctctg caggcggtcc tattctgacc ccctctctgg atgcgattac cctggtgccc
     8161 atgttcccgc atacgttgtc agcacgacca ctggtcataa acagcagcag cacgatccgt
     8221 ctgcgttttt cgcatcgccg taacgacctg gaaatcagtt gcgacagcca gatagcactg
     8281 ccgattcagg aaggtgaaga tgtcctgatt cgtcgctgtg attaccatct gaatctgatt
     8341 catccgaaag attacagtta tttcaacaca ttaagcacca agctcggctg gtcaaaaaaa
     8401 ttattctaat tttacgccag cctctttact gtatataaaa ccagtttata ctgtacgcaa
     8461 taacagtaat ggtttttcat acaggaaaac gactatgttg gcacaactga ccatcagcaa
     8521 ctttgctatc gttcgtgagc ttgagattga ttttcatagc ggcatgaccg taataactgg
     8581 cgagaccggc gcgggtaaat ctattgcaat agatgccctc ggtctttgtc tcggtggtcg
     8641 cgctgaagcc gacatggtgc gtaccggcgc tgctcgcgct gacctgtgcg cccgtttttc
     8701 tctgaaagat acgccagcgg ctctgcgctg gctggaagaa aatcagcttg aagacgggca
     8761 tgaatgtttg cttcgtcgcg taatcagcag cgatggtcgc tcccgtggtt tcatcaacgg
     8821 tacagctgtt cctctgtcac aactgcgcga actgggtcag ttgctgattc agatccatgg
     8881 tcagcacgct catcaattac tcaccaaacc tgagcaccaa aaattcctgc ttgatggcta
     8941 tgccaatgaa acctctctac tgcaggaaat gaccgcacgt tatcagttgt ggcatcaaag
     9001 ctgccgtgac ctcgcgcatc atcaacagtt aagtcaggaa cgcgccgccc gtgcggaact
     9061 gctgcaatac caattaaaag aacttaacga atttaatccg cagcccggag agtttgaaca
     9121 aatcgacgaa gagtacaaac gtctggcgaa cagcggtcaa ttgctgacca ccagccagaa
     9181 tgcattggca ttaatggcct acggtgaaga cgcaaacctg caaagtcagc tttacacggc
     9241 taaacaactg gtgagcgaat tgattggcat ggacagcaaa ctgtccggcg tacttgatat
     9301 gctggaagaa gctaccatcc agattgctga agccagcgat gaactgcgcc actactgcga
     9361 tcgtctggat ctcgatccca accgactatt tgaacttgaa cagcgcatct caaaacagat
     9421 ttcgctggca cgtaaacatc acgtcagccc tgaggcattg ccacagtatt accagtcgct
     9481 actggaagaa cagcagcaac tggacgatca ggccgactca caagaaacgc ttgcgctggc
     9541 ggtaacgaaa catcatcagc aggcactgga aatcgcgcgc gcattacacc aacaacgcca
     9601 gcaatatgca gaagaacttg cacagctgat caccgacagt atgcatgcgc tctcaatgcc
     9661 gcatgggcag tttacgatcg atgttaaatt tgacgagcat cacctgggcg ctgacggtgc
     9721 cgatcgtatt gagtttcggg taaccaccaa cccaggtcag ccaatgcagc ctattgccaa
     9781 agtcgcatcc ggtggtgaat tgtcccgcat cgcactggca acccaggtca tcacggcgcg
     9841 taaaatggaa accccggcac tgatttttga tgaagtggat gtagggatta gcggtccaac
     9901 agcggcagtt gtcggcaaac tgctgcgtca actcggcgaa tcaactcagg tgatgtgtgt
     9961 tacccacctg ccacaagtcg cgggatgtgg tcatcaacac tattttgtca gcaaagaaac
    10021 cgatggtgcg atgacagaaa cgcatatgca atccctgaat aaaaaagcgc ggttacaaga
    10081 gctggcgcgc ctgcttggtg gcagtgaagt cacacgtaat acactggcga atgcgaaaga
    10141 actgcttgca gcgtaaactt ttttcctgct tcacggtcag agtaaacagc aaaacgccgt
    10201 aagaccggaa agcaaaaggt tttaaagtga tgaaaggtct attatcatcg gcatattaca
    10261 gatgagccac gtactgctag ggcccgaaaa ggaatcaaat cactatgcgc tgtaaaacgc
    10321 tgactgctgc agcagcagta ctattgatgt tgaccgcagg ctgttccact ctggagcgag
    10381 tggtttaccg tcctgacatc aaccagggga actatctgac cgctaacgac gtatccaaaa
    10441 tacgtgttgg catgacgcaa caacaagttg cgtacgcatt gggtacaccg ctgatgtccg
    10501 atccatttgg tacgaatacc tggttctatg tcttccgcca gcaaccaggt catgaaggtg
    10561 taacgcagca aacgctgacg ctgaccttta acagtagcgg tgtgttgacc aatattgata
    10621 acaaacctgc gctgagtggt aactaataat aaagttgctc tcaaagacgt taaaaaaggt
    10681 gctcaatgag cacctttttt ctgtctgtta tttattcgct gatttttctg ctcgttgcct
    10741 gcgaagctct ttcggatcgg caatgagagg gcgataaatc tccacccgat cgccatcatg
    10801 cacactatcg cttagttttg ccggacggct gtaaatgccg actttgtttt tagttaaatc
    10861 gatatcggta cgcaattcca gcaagccact ggcgcgaata gcttcttcaa ccgtcgcgcc
    10921 ctcctgcagc gtcacgcgct gcaggtactg cttctcaggt agcgcataag ccacctcaac
    10981 ggcaatttta cctggcactg taaacctctt tcgcacgaac cgtaaaagcc tggaccatat
    11041 tagacgccag ctctttaaac acgcgaccaa aggcgagttc aatcaactta ttggtaaact
    11101 caaagtcgag atgaaactcg atacgacacg cctcctggct cagcggcgta aacttccatc
    11161 caccaatcaa tttcttgaac ggcccatcca ccagattcat aagaatactt tggttactgg
    11221 tcaactggtt gcgggtagta aacgttttgc tgatcccagc cttagagaca tctaccgccg
    11281 cagtcatctg cccaggagtg gactccagaa tccgacttcc ggtacaaccc ggcaaaaact
    11341 gaggataaga ctgaacgtca ttcactaact gatacatttg ctccgcgctg tagggtacca
    11401 gtgcggtccg gctaatctga ggcataacta tttccatcaa caaaaatcca acaaataata
    11461 tcatttatcc tgctgtaaaa aaaacgctat cccggcgctg ggtaacatcg ggttcatgct
    11521 aagatagagc cttgtccccc gcaggattga tatggggtgt tttcgatttc agattaccga
    11581 tgattcacga cgcttatgac gaagaaaaaa gcacataaac ctggttcagc gaccatcgcg
    11641 cttaacaagc gcgcccgtca cgaatacttt atcgaagaag agttcgaagc gggacttgcc
    11701 ctgcaaggct gggaagttaa atccctgcgc gcaggaaaag ccaatatcag cgacagctac
    11761 gtccttctgc gtgacggaga ggcatttctg tttggcgcta acatcacgcc aatggccgtg
    11821 gcctccacgc atgtggtgtg cgatcctacc cgtacccgca agttacttct caaccagcgc
    11881 gaactggact cattgtacgg tcgcgtcaat cgagaaggct ataccgtagt ggcgctctcc
    11941 ctgtactgga aaaatgcctg gtgcaaagtg aaaatcggcg tcgccaaagg taaaaaacag
    12001 cacgataaac gttcagatat caaagaacgc gaatggcagg tggataaagc acgtatcatg
    12061 aaaaacgccc accgttaaac ctgcactcca attattgacc agttcctcac cgcgcctccc
    12121 tctccggcgg cgcgaatgaa catcttattg gctatcacat ccgacacaaa tgttgccatc
    12181 ccattgctta atcgaataaa aatcaggcta catgggtgct aaatctttaa cgataacgcc
    12241 attgaggctg gtcatggcgc tcataaatct ggtatacttg ccgttacaca ttggggctga
    12301 ttctggattc gacgggattt gcgaaaccca aggtgcatgc cgaggggcgg ttggcctcgt
    12361 aaaaagccgc aaaaaatagt cgcaaacgac gaaaactacg ctttagcagc ttaataacct
    12421 gcttagagcc ctctctccct agcctccgct cttaggacgg ggatcaagag aggtcaaacc
    12481 caaaagagat cgcgtggaag ccctgcctgg ggttgaagcg ttaaaactta atcaggctag
    12541 tttgttagtg gcgtgtccgt ccgcagctgg caagcgaatg taaagactga ctaagcatgt
    12601 agtaccgagg acgtaggaat ttcggacgcg ggttcaactc ccgccagctc caccaatcat
    12661 gattggacgg tataaggaca acaccaataa aaacaggagg ttagcagtct cagcaggaca
    12721 ccgaccagac ggtgaagaga cataaaagga tacgcaaagg agccgcggct cctggtgaca
    12781 tgaaagccca cagatgtggg ctttttcgtt gatggtcaga acgaccagtt cacaccagct
    12841 accccgttcc acggggattc cacaccggca ccatggctat accccacccc aagatgcccg
    12901 cttaacgtac tgctgaatga ggctttaata cctgcctggt atattccacg tctgcccgac
    12961 aaatcattga cgaaattacc gtcactattc actttcaccc ggttatcatc gacaaattct
    13021 ttgcgcacag ccgccttcag ccacggctca acttccatac cgttccccag acgcatgttg
    13081 taactcagcg ttgcacccag ttcacgatat aaacctgcgg gtatcgactg atttcgattc
    13141 catgccattg gataaatgat attcagggtt atcagcggtg aacccccgtt aacgatgcat
    13201 acggcgtcag gttccagtta ccatcggtaa atcgcatccc ggtttcaatg tgaccgccca
    13261 gcccgttgct gcgataactg ccgttggtgg ctccaccgct gccaaccgtg gagtcgcgag
    13321 cttcggtacc tgctaatacc aataaattac cgccattttc cagcaacata ttggtcgcta
    13381 aattgccgga aatggaaaaa gtgccgtact ggtaagtacc gctgatttca ataccgttag
    13441 ccgtgctcgt ctggagagcg gcaccgctgt tctggacgat atctgtcgct ttgccattat
    13501 cgttaactgt cagcgtaccg ccttcattga tctttgtttt tattgcctct ccgttagctg
    13561 aaactgtttg tattccgccg tcgttaatcg ttgtctcatt cgccaccccc tcgacgattt
    13621 gttcgccgcc ggtgagcgtc gtgcctgtcg cagtggcttt tgttttgacg atctcccgtc
    13681 cgcccgtatt gacctgtgtt ttgtcagaag aggtgtctga ctccacggtt aacacgccgc
    13741 catttgccag caggatattg ttcgccgcac cctgctcgat gctgaacgcg acgccatccg
    13801 cgcgtgttcc tgtgacccgc gtcgccctgg tggttgcaac caaagcgccc tggctactct
    13861 gctgtatccc cgttgcgctg cctttctccc gcacatcgag tgtgccgccg tcattaagca
    13921 ccgagttttc agccagaccg ccctcattaa ctacctgtga acccccatta ataatggaac
    13981 cttccgctgt cccgtttgcc ataatttgtt gtaggccaga gacgatatcg gtattgattg
    14041 ccttaccata attctgaacg gtttgcgtgc caccattgat gtgtgttttc tctgttgacc
    14101 caccatcaac aatttgttca ccactttcga tatttgcctc agtggctaat ccatataccg
    14161 tttgcttgcc acctttgata tttgctttat cagaagtggc actggcatat attgtttggg
    14221 tgccagcact attgagtaca gtgccaacat cttttccata aacatccatt ttgccgttgg
    14281 cattaataat cgtatcaact gcccgggaac cagtgacgac tgttaatgag ccagcgtttt
    14341 ccagcactac atttttagct tctgaattcc tgatgtagaa agcatcacca taactgttgg
    14401 ttcctttgat aagggttccg gaagttgtgg aagcaattaa tgcgccgccg gattgttgct
    14461 caacatgctt agcctcacca ccgtcctgaa cctccagaac gccaccatta ttaagtctgg
    14521 tggtatctgt tttagcctcc ttctggacaa tcagcttacc gcca
//
LOCUS       DAJTMC010000099        14563 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.099, whole genome shotgun sequence.
ACCESSION   DAJTMC010000099 DAJTMC010000000
VERSION     DAJTMC010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14563
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.099"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OO130_002692"
     CDS             complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OO130_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR5366039.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(280..2394)
                     /gene="fusA"
                     /locus_tag="OO130_002693"
     CDS             complement(280..2394)
                     /gene="fusA"
                     /locus_tag="OO130_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCR5366040.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="OO130_002694"
     CDS             complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="OO130_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HCR5366041.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="OO130_002695"
     CDS             complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="OO130_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HCR5366042.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="OO130_002696"
     CDS             complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="OO130_002696"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HCR5366043.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQISNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="OO130_002697"
     CDS             complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="OO130_002697"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HCR5366044.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLGLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="OO130_002698"
     CDS             complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="OO130_002698"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HCR5366045.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIVEGHELSSVFFYRGGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4598..5320)
                     /locus_tag="OO130_002699"
     CDS             complement(4598..5320)
                     /locus_tag="OO130_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR5366046.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="OO130_002700"
     CDS             complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="OO130_002700"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR5366047.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6520..6738
                     /locus_tag="OO130_002701"
     CDS             6520..6738
                     /locus_tag="OO130_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HCR5366048.1"
                     /translation="MQDLSLEARLAELESRLAFQESTIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="OO130_002702"
     CDS             complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="OO130_002702"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR5366049.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7472..7672)
                     /locus_tag="OO130_002703"
     CDS             complement(7472..7672)
                     /locus_tag="OO130_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HCR5366050.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(7682..9493)
                     /gene="kefB"
                     /locus_tag="OO130_002704"
     CDS             complement(7682..9493)
                     /gene="kefB"
                     /locus_tag="OO130_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HCR5366051.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEE
                     DEKPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYY
                     GDATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHE
                     LLQAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHA
                     DTVQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9493..10044)
                     /gene="kefG"
                     /locus_tag="OO130_002705"
     CDS             complement(9493..10044)
                     /gene="kefG"
                     /locus_tag="OO130_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HCR5366052.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     KELANHARAYGDWLANPLSPGGR"
     gene            10175..12088
                     /locus_tag="OO130_002706"
     CDS             10175..12088
                     /locus_tag="OO130_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5366053.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALSQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12088..13110
                     /locus_tag="OO130_002707"
     CDS             12088..13110
                     /locus_tag="OO130_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR5366054.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEAPAQARHKPRLVIFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPSQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13104..13322
                     /locus_tag="OO130_002708"
     CDS             13104..13322
                     /locus_tag="OO130_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HCR5366055.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13376..14245
                     /locus_tag="OO130_002709"
     CDS             13376..14245
                     /locus_tag="OO130_002709"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HCR5366056.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14300..>14563)
                     /locus_tag="OO130_002710"
     CDS             complement(14300..>14563)
                     /locus_tag="OO130_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HCR5366057.1"
                     /translation="SAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIV
                     TGRDLKDAAVARAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
BASE COUNT         3601 a         3971 c         3585 g         3406 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcacgagc agcaccgccg taggttttag ccagtacggt ggtgattgca
      121 gcggtcagag tagttttacc gtggtcaacg tggccgatag taccaacgtt aacgtgcggt
      181 tttgtacgtt caaatttttc tttagacacg gctatattcc ttactatagt gctctcccct
      241 tcaggagaga gcacgggact ttggtattaa cccttaggct tatttaccac gggcttcaat
      301 tacggcctga gcaacgttac tcggcgcttc atcatacttc aggaattcca tagtgtatga
      361 tgcacgacct ttggtcagag aacgcagctg agttgcgtat ccgaacattt cagacagcgg
      421 tacttcagcg tggatcttaa cgccagtaac ttcagattcc tgacctttga gcataccacg
      481 acgacggctc aagtcaccga taacgtcacc ggtgttctct tccggagttt ctacttcaac
      541 cttcatgatc ggctcaagca gaactggttt cgctttctta aagccttctt taaaggcgat
      601 agaagcagcc agtttaaacg ccagttcaga ggagtcaacg tcatggtaag aaccgaagtg
      661 cagacgaata cccatgtcta ctaccgggta gcctgccagc ggacctgctt tcagctgttc
      721 ctggatacct ttatcaacgg ccgggatgta ttcgccaggg attacaccac ctttaatgtc
      781 gttgatgaac tcgtagcctt tcgggtttga acccggctcc agcgggtaca tgtcgataac
      841 aacatgacca tactgaccac gaccaccaga ctgtttcgcg tgtttacctt caacatcagt
      901 aactttctgg cggatagttt cacggtaagc aacctgcggt ttacctacgt tcgcttcaac
      961 gttgaattca cgcttcatac ggtcaacgat gatgtcgagg tgcagttcgc ccatacccgc
     1021 gatgattgtc tggttagatt cttcgtcagt ccatacacgg aaagacgggt cttctttagc
     1081 cagacggccc agagccagac ccattttttc ctggtcagct ttggttttcg gttcaactgc
     1141 gatggagatt accggctcag ggaattccat acgttccaga atgatcggcg catccgggtc
     1201 acacagggtg tcaccagtgg ttacgtcttt cagaccgata gcagcagcga tgtcgcccgc
     1261 gcgaacttct ttgatctctt cacgtttgtt agcgtgcatc tgaacgatac gaccgaaacg
     1321 ctcacgtgca gctttcacgg agttcagtac ggtatcacca gagttaacca caccggagta
     1381 aacacggaag aaggtcaggt taccaacaaa cgggtcggta gcgattttga acgccagtgc
     1441 agagaacggc tcgtcatcac ttgcgtgacg ttcagccgga gtgtctttac cgtcgtccag
     1501 gataccgttg atcgcaggta cgtcaaccgg ggatggcagg taatcaatta ccgcatccag
     1561 catcgcctga acacctttgt tcttgaacgc agaaccacag gttaccagga tgatttcgtt
     1621 gttcagaacg cgctgacgca gagcaccttt gatttctgct tcagtcagtt cttcaccacc
     1681 caggtatttt tccatcagct cttcagaagc ttcagctgcg gattcgatca ggttctggtg
     1741 ccattcgtta gccagttcaa ccatgtctgc cgggatatct tcgtattcga aggttacgcc
     1801 ctggtcagcg tcgttccagt taatagcttt cattttcacc aggtcaacaa caccggtgaa
     1861 atgttcttca gcaccaatcg ccagctgcag cggaaccgga ttcgcgccca gacgggtttt
     1921 gatctggtta acaactttca ggaagttcgc acccatgcgg tccattttgt taacgaacgc
     1981 aatgcgcgga actttatatt tgtttgcctg acgccatacg gtttcagact gcggctgaac
     2041 accaccaact gcgcagtaaa ccattaccgc accatcgaga acacgcatgg aacgttctac
     2101 ttcgattgtg aagtcaacgt gccccggggt gtcgatgatg ttgatgcgat gcggctcata
     2161 ctgcttagcc ataccagacc agaatgcagt agtcgcagcg gaagtgatgg taataccacg
     2221 ttcctgctcc tgctccatcc agtccatggt tgcagcgccg tcatgaactt cacctatttt
     2281 atggtttaca ccggtgtaga acagaatacg ttcggtagta gtggttttac cggcgtcgat
     2341 gtgcgcactg ataccgatgt tacggtagcg tgcgatgggt gttgtacgag ccatttgttt
     2401 cctcgtttat cttttaggcg ttcaatttaa gtagcccaaa gcgggctgct tactggaagc
     2461 gcccgcctgg tgactaaaac tccgaaggga ttaccaacgg tagtgtgcga acgccttgtt
     2521 ggcttcggcc atacggtgaa cgtcttcacg tttcttaact gcagtacctt tgttttctgc
     2581 agcatcagaa agttcgttcg ccaggcgcag agccatggat ttatcaccgc gtttacgagc
     2641 agcttcaacg atccaacgca ttgccagagc attacgacga accggacgga cttcaactgg
     2701 tacctgataa gtagaaccac caacgcggcg agacttaact tctacagtcg ggcgcacgtt
     2761 ttcgagagct acttcgaatg cttccagttc agatttacca gagcgctgag ccagggtctc
     2821 cagcgcgctg tatacgatag attcagcagt agatttttta ccatctacca tcaggatatt
     2881 tacaaattta gccagcagtt ctgatccgaa cttcggatcc ggcagaattt tacgctgacc
     2941 aatgacgcga cgacgtggca tggaaatact ccgttgttaa ttcaggattg tccaaaactc
     3001 tacgagttta gtttgacatt taagttaaaa cgtttggcct tacttaacgg agaaccatta
     3061 agccttagga cgcttcacgc catacttgga acgagcctgc ttacggtctt taacgccgga
     3121 gcagtcaagc gcaccacgta cggtgtggta acgaacaccc gggaggtctt taacacgacc
     3181 gccacggatc aggatcacgg agtgctcctg caggttgtga ccttcaccac cgatgtagga
     3241 agtcacttcg aaaccgttag tcagacgaac acggcatact ttacgcagcg cggagttcgg
     3301 ttttttagga gtggtagtat atacacgagt acatacgcca cgtttttgcg ggcatgcttc
     3361 cagcgcaggc acgttgcttt tcgcaacttt gcgagcacgt ggtttgcgta ccagctggtt
     3421 aactgttgcc attaaatagc tcctggtttt agcttttgct tcgtaaacac gtaataaaac
     3481 gtcctcacac aatatgagga cgccgaattt tagggcgatg ccgaaaaggt gtcaagaaat
     3541 atacaacgat cccgccatca ccaggccatc tggctggagt gcttaaccgt aagtctgacg
     3601 aaatcagtat agtcaatggg aatgatgtcg ttcgaaattt gaccagtcaa accgcgggca
     3661 ataaggtctt cgttcagggc atagacctta atgggggcat tacgcagact ttcaaggtag
     3721 cggttaccgt caactgcggc agttacgcca tcttgcaata atagcagttc gtctccttca
     3781 ctgagcagac gcagcagcgc agcaaaatcc gtcagccagg gtgaacgatg taacgtgtgc
     3841 agcataaacg cctcaaaacc tcaaaataac atcgtagttg gcgagttcgc ggcgtaaggc
     3901 atctgcttcg agcggcgtgg cttcgacaac aaagggtgtc tgcggatcta acccgcgttc
     3961 gcgcagtgaa gccgcacaaa cccagcactg ttcaatgtcg tacagaccca acaatttaaa
     4021 agtggcaatg taatcacgcg ccagcactgc atcgggcttt tgtcctgaca gcagctgaaa
     4081 aacgccatca gcaataaaga agacggccag atcgtcagtt aatgcggaag ttgccagtaa
     4141 agcatctaaa ccttcccgac cagcagctgt accatgaggt gcagtagaaa aaacaaacgc
     4201 aattcgtttc atcagaactg taccaccctg tcacaggtca gcgaggcttc cgccagcgcg
     4261 ccaagtccgc ttaaggtaaa tcccggctga aggtttgacg aagccagccc cagccttcct
     4321 gcttcagttt catcgacgat accgcggcgt aacgctgccg ctacgcagat attcagcgcc
     4381 acaccatgtt gcgcattcaa ctgttgccag ccgcgcacaa ggtcaaattc gtcacttgcc
     4441 ggtgaggtca attggttagc gttatagacc cctccccgat agaaaaagac gctgcttaac
     4501 tcatggcctt ctactatcag cgcctgcgca aactgaaaag cactactcgc ctgttgcgta
     4561 ccgtatgctg gcccggtcac cacgatggca aaacgcatta cttatcttgc ccctggaaat
     4621 caccgctctt gaactggcgg atgtagagat agacagtgtg tttggagatg ttcaggcggt
     4681 cagcaacctg gttgatcgca tctttaatat cgaagatccc tttctcgtag agattcagca
     4741 cgatctgacg atttttggcg ttattagaaa cattgcgatc ggcattcact tcttcgatgg
     4801 tgaactccag cgtttgggta accagatctt caacagaaga ggcaaagttg acgcttgaac
     4861 cgacatccgg ggtttctggc ggcacaaaag tgctcataat ctgcgagaag ggaacatcaa
     4921 gattcatatt gatgcacagc agaccaatta cacgctgttc gcggttacga atcgcgatag
     4981 tcagggactt cattaatacg ccgcttttgg cgcgagtaaa gtagcattta gaaacgctgc
     5041 tatccgctcc cgtcatatcg tgcagcatac gtagcgccag gtcagtaatt ggcgaaccaa
     5101 tcttccggcc tgtatgttca ccgttagcaa tgcgaatggc tgaacatttt agatcctgca
     5161 aagagtgcaa aacgatttca cagtgggagc caataagcat cgctaacccg tccaccaccg
     5221 cttcgtagga tttcagaata tcaaaatcgg tctggtcgaa aggacgttga tccagtaaat
     5281 ccaactcact ggtttcgttg gttaaaagcg acctggacat gaaaaaaaac actccttttc
     5341 aggagcctgt cgttaacttt tcagggcagg ctcattaatg atgcgggtaa ctaaattaat
     5401 acagcggagg ttccgctttc cagcactaat tatatccggc ctgtaataaa aaaaccgccg
     5461 cctggtcagg cggcggttct taatgcttat tttttagcag aatctgcggc tttcgcatca
     5521 gcttccggct ttgcatcagc cttcggtgct ggtttcacat ccagcagctc tacgtcaaac
     5581 accagggtag aattcggtgg gatccccgga acacccgctt tgccgtaagc cagttctggt
     5641 ggaataacca gtttgatctt accgcctttc ttgatgttct tcagaccttc tgtccaaccc
     5701 gggataacac cgtccagacg gaaagaaagc ggttcaccac gggtgtaaga gttgtcgaac
     5761 tctttaccgt cgatcagcgt acctttgtag ttcactacaa cagtatcgct gtctttcggt
     5821 gcttcgcctt taccggcttc tactacctga taaaccagac cagttgaaga ggttttcaca
     5881 cctttctctt tggcaaattt ctcgcggtac tctttacctt ttgcttcgtt atcagccgcg
     5941 tctttttcca tcttcgcctg agcagaagac ttcacgcgag cttcgaatgc ttgcagagtc
     6001 tgttcgatct cttggtcgga gagtttgctc ttatcagcaa atgcatcctg aacaccagcg
     6061 atcagctgat ctttatccag tttgatgccc agtttttctt gttctttaag agagttttcc
     6121 atgtaacgac ccagcgaagc acccagtgca taagctgatt tctgatcgtc atttttgaac
     6181 gctgctttgc tgtcagcagt tgtagcaggt tttgcagctt cagcagcaaa agtgattggt
     6241 gcatgcaggg caacggccat tgtggtcgcc agcagcgtta ctttaaacag tgatttcatc
     6301 catatctcca ggatcggggc atctcacccc agggttaact attatcagaa gggtactata
     6361 aagcgttgtc gaacaaatct acatacagac acgccctatt atcatctatt ttcagactct
     6421 ttttgtttaa attagtttcg atgaccgcga aatgagtgct gtctcgggca gcaaagttaa
     6481 gtagaatccg cggcaaccat tcgacaaaag aggtgaatca tgcaggattt atcattggaa
     6541 gcacgcctgg cagagctgga gagccgactg gcttttcagg aaagcaccat tgaagaactg
     6601 aacgtcacgg tgaccgctca tgaaatggag atggcgaaac tgcgcgatca tctgcgtctg
     6661 ctgaccgaga agttaaaagc cagccagccg tcgaacatcg cgtcgcaggc tgaagaaacg
     6721 ccaccgccac attattgagg cgtaaaaaaa gcggggattc cccgcttttt tgtcaccttt
     6781 tcggtattag tggcaaccac aaccgccgtt gcctttaccg ccacagcagc cttcgccacc
     6841 gtgttcatga ccgtgatcgt ggccatgacc gccgcagcaa ccgtcgtggt cgtgatcgtg
     6901 gtgatgatcg tgcgcgccgt gaacgtgacc atgagccagt tcttcttcgg tcgcttcacg
     6961 aatcgccaca acctcaacgt tgaatttcag gttctgaccg gccagcatgt ggttaccatc
     7021 aaccactacg tgatcgtctt caaccgcagt gatttcaacc ggtaccggcc cctggtcggt
     7081 ttcagccagg aaacgcatac ctacctgcag ttcatcaacg cccataaata cgtctttagg
     7141 aacacgttgc accaggtttt cgtcgtactg accgtaagcg tcgttcgcgc caacagcgac
     7201 atcaaatttg tcgccaactt catgaccttc cagcgccgtt tccaggccag agatcaggga
     7261 accgtgacca tgcaggtagt ccagcggcgc actcaccgga gactcatcaa ccaacacacc
     7321 gtcttctgta cgtacctgat aggccaggct gaccaccaag tcttttgcta ctttcatgat
     7381 atctcctgag catgggaaga atagtggcgc agattgtagc ggaattctgc agccgtgtac
     7441 tcactagctt aaaaaaactc ggcgcatatc gctagtccgg atgaaaaatc ccgatcactt
     7501 gctcatcttt gcgaacgtga tcgcgggctt ctttgtctgc ttctcgcatc tgatgtccgc
     7561 acttaacgca ttcaacaata tcaatattat tttcgcgcca catcgccatt gaatcctgtg
     7621 cctgacaggc cgggcatttt gcgcccgcaa taaaacgttt tcggattgcc atctttaccc
     7681 tctactcaaa ttcatcccag ccgtccagct ggcgtcgttc ttgttgcatt tcacgctgga
     7741 aaatctcttc cagttcgcgt cgggcttccc tggcgcgaga aatttgtaca gtatcggcat
     7801 gcattgggat gagctctcgc agcatccgca tatccaggcg gcgaaaatgc agttgcgccc
     7861 gctgcgcctg atgcggatgc atcccaagcg tgaccaaagt cttgcgcccc agctctaacg
     7921 cactggagaa tgtttcacgg gaaaactgcg tcacccctgc ctgtaataac tcatgcgctt
     7981 ccacacgtcc gcgcgctcgc gcaagaatat gcaaatgcgg aaagtgctgc tggcatattt
     8041 ccaccagctt catggtgtct tccggctcgt tacaggtaat gacgatagac tcagcggcct
     8101 ctgcacccgc agaacgtaat agatcgacct gcgtggcgtc gccgtaataa actttgtagc
     8161 catatttacg catcaggtta acggcgctga tatcccgctc cagcacggta atgcgcattt
     8221 tatttgccat cagcaaacga ccaatcacct gaccaaaacg cccgaagccc acgacaatga
     8281 cctggggttt atcatcgttg acccacggtt tttcgtcttc ttcttccggt ccgttaaact
     8341 ggcgggataa ccatttatcc accagcttca tcagcaacgg cgtggtcatc atggaaagcg
     8401 tcaccgtcac cgtcaccagc aacagcgcca tctggtcgcc ctggaataaa cgttgtgaag
     8461 aagcggtaga aaagaggaca aaggcaaact caccgccctg actcaacacg ccagcaaact
     8521 gcatccgctc tgagctacgc acgccataca atcgcgccag cagatacagc acgagaattt
     8581 tcaccgccac cagcacaacc acgcttatca ctacccacaa cagatgggta taaagcaccc
     8641 cgaggttgag cgacatgccg acagagataa agaacaaacc gagcagcaag cctttgaagg
     8701 gatcgatagc cgtttccagt tcatggcgat attcactttc cgccagcagc acgcccgcaa
     8761 taaatgtacc gagcgccatc gacagcccca gcgcatccat aaacaatgcg gaacccaaca
     8821 ccagcagcag cgtcgcggcg gtgaacactt cccgcacgcc agaagctgca ataaagcgga
     8881 ataccggacg cagtaaatag cgcccaccaa tcagcatgcc gacaaacgcc agcaccttca
     8941 tgccgatctt catccagtcg aaatgttcgt ctgccgaccc cgccaacaac ggcactaacg
     9001 ccagtgctgg gatcactgcc agatcctgaa acagcagaac cgaaaacccg agctggccgg
     9061 attcgctgcg attcatccct ttctcacgca tcaattgcaa cgccattgca gttgaagaca
     9121 tcgcaaggcc aatgccaccg accaccgccg cctgccaggc gaaatccgtc agcatcaata
     9181 atcccgccag caacgccgcg cttaacagca cctgtgccgc gcctacgcca aaaatcgaac
     9241 gccgcagttg ccaaagtttg gagggattca actcaaggcc gatgataaac atcaggaata
     9301 ccacgccgag ttccgaaaag tggaggatct catcgacgtc gctaataaac cccagccccc
     9361 acgggccaat tgcaatccct gccagcaaat atcccaacac agcgccaatc cccagccgcg
     9421 atgccaacgg caccgcagcc accgccgcga agagaaacag cactcctgcg agtaaaaaat
     9481 cggaaccttc catcagcggc ctcctggaga cagcggattt gccagccagt caccgtaggc
     9541 tctggcgtgg ttcgccagct cctttgcgct ttgccgtctc gcccagtaaa taatgatggg
     9601 acttaaccaa tgcatccggc acatgcccgc cgccagttca aaggggcgca gcacatcgct
     9661 catcgggtag cgattcagcg cgtcataacg gtaagcactt tccggctcgc cggtggtaat
     9721 cacgttacgc cagtactttc ccgccagttg gtttcctccc ggcccgctgg caaaaccacg
     9781 acttaatacc cggtccagcc actctttcag tagcgccggg cagctatagg tataaagagg
     9841 atgctgaaag acaatcacct cgtgctcgcg cagtaatgcc tgctcacggg ggatatcaat
     9901 aaaaaaatcg ggatagtgcg cgtaaaggtc gtgcacggta acattgctga gctgcgtggc
     9961 cggtttaagc agtacccggt ttgccaccga gtcctgagat tccggatggg catacagcag
    10021 caaaactttc gctggctgag acatcatccc cctcccggta tggtttttgt gtatagtcgc
    10081 tgttttgggc taccattgcg cccggtgcgg cagctcgccc atacattaca ttatcataat
    10141 gataagttaa catagtctga acatacggcg ccttatgatt gttttctcct cgttacaaat
    10201 tcgtcgcggc gtgcgcgtcc tgctggataa tgccaccgcc accatcaacc ctgggcagaa
    10261 agtcggcctg gtgggtaaaa acggctgtgg taaatctacc ctgctggcat tgctgaaaaa
    10321 tgaaatcagc gccgacggcg gcagctacac ctttccggga agctggcaac tggcgtgggt
    10381 gaatcaggaa acgccggcgt tatcgcaagc ggcgctggaa tatgtcattg acggcgaccg
    10441 tgaatatcgt caactggaag cgcagctgca cgacgccaac gaacgtaacg acgggcacgc
    10501 cattgcgacc attcatggca agctggatgc tattgacgca tggagtattc gctcccgtgc
    10561 cgccagcctg ctgcacggcc tcggtttcag caatgaacaa ctggagcgcc cggtaagtga
    10621 tttctccggt ggctggcgta tgcgtcttaa ccttgcccag gcgctgattt gccgttcaga
    10681 cttgctgctg ctcgacgaac cgactaacca cctcgatctc gatgccgtta tctggctgga
    10741 aaaatggttg aagagctatc aaggcacgct gatcctgatc tctcacgacc gcgacttcct
    10801 cgatccgatc gttgataaaa ttattcatat cgaacaacaa agcatgttcg agtacaccgg
    10861 caactacagt tcgtttgaag tacagcgcgc cacccgtctg gcgcaacaac aagcgatgta
    10921 tgaaagccag caggaacgcg tggcacatct gcaaagttat atcgaccgtt tccgtgccaa
    10981 agccaccaaa gcgaagcaag cccagagccg cattaagatg cttgagcgta tggagctgat
    11041 tgcccccgcg cacgtcgaca acccgttccg ctttagcttc cgcgcgccgg aaagcctgcc
    11101 aaatccgtta ctgaagatgg aaaaagtcag cgcgggctat ggcgatcgca ttattctcga
    11161 ctcgattaaa ctgaacctgg tgcccggctc gcgcattggt ctgttaggcc gcaacggcgc
    11221 gggtaaatcg acattaatca aactgttagc cggtgaactt gcgccagtca gcggtgaaat
    11281 tggtctggcg aaagggatca agctcggcta cttcgcccag catcaacttg aatacctgcg
    11341 cgccgacgaa tcacctattc aacatctggc acgtttagcg ccgcaggagc tggaacaaaa
    11401 actgcgtgac tacctcggcg gctttggttt ccagggcgat aaagtaaccg aagaaacgcg
    11461 ccgcttctcc ggtggggaaa aagcccgcct ggtgctggca ttaattgtct ggcagcgtcc
    11521 gaatctgctg ctgctcgacg aaccgactaa ccaccttgac ctcgacatgc gtcaggcact
    11581 caccgaagca ttaatcgagt ttgaaggcgc gctggttgtc gtttcgcacg accgtcattt
    11641 gctgcgttcc accactgacg atctctacct ggttcacgat cgtaaagtcg aaccgttcga
    11701 cggcgatctg gaagattatc aacagtggtt gagcgacgta caaaagcagg aaaaccagac
    11761 cgacgaagcg ccaaaagaga acgcgaacag cgcccaggca cgtaaagatc agaagcgtcg
    11821 ggaagcggag ctgcgtgcgc aaacccagcc gctgcgtaaa gagattgccc gtctggaaaa
    11881 agagatggag aagctgaacg cgcaactggc gcaggcggaa gagaaactcg gcgacagcga
    11941 actgtatgac cagagccgta aagcggagtt gaccgcctgc ctgcaacagc aagccagcgc
    12001 caaatccggc ctggaagagt gcgaaatggc gtggctggaa gcccaggagc agcttgagca
    12061 gatgctgctg gaaggccaaa gcaactgatg gcgcagataa cgacgaccga tgccaatgaa
    12121 ttcagcagca gtgctgaatt cacccctatg cgcggcttta gcaattgtca tctacaaact
    12181 atgctgccgc gtttgtttcg tcgtcaggtg aaattcaccc cgtactggca gcggctggag
    12241 ttgcccgacg gcgattttgt cgatctcgcg tggagtgaag cccctgcaca ggctcggcat
    12301 aaaccgcgtt tagtgatatt tcacgggctg gaaggcagtc tcaatagccc ttacgcccac
    12361 ggtctggttg aggcggcgca aaagcgcggc tggctgggcg tggtgatgca ttttcgcgga
    12421 tgcagcggtg aaccaaaccg tatgcaccgc atttaccatt cgggcgaaac cgaagacgcc
    12481 agttggtttt tacgctggct gcaacgcgaa tttggtcatg cgccaacggc tgccgtcggc
    12541 tattcgctcg gcggtaatat gctggcctgt ttgctggcaa aggaaggcaa tgatctcccg
    12601 gttgatgcgg cggtgattgt ctctgcgccg tttatgctgg aagcctgtag ctatcatatg
    12661 gaaaagggct tttcccgcgt ttatcagcgt tacttgctga acctgttaaa agccaatgcc
    12721 gcgcgcaagc tggcagccta ccccggaacg ctgccgatta atctcgcgca gttaaaatcg
    12781 gtacgtcgca tccgtgaatt tgacgatcta atcaccgcca gaattcacgg ctacgccgac
    12841 gctatcgact attatcgtca gtgtagcgcc atgccgatgc tgaaccggat cgccaaaccg
    12901 acgctgatta ttcacgccaa agacgatccg tttatggatc atcaggtgat cccgaaaccg
    12961 gaaagtctcc cctcgcaggt ggagtatcaa ctgactgaac atggcggtca tgttggcttt
    13021 attggcggta cattacttca tccgcaaatg tggctggagt cacgcattcc tgactggtta
    13081 acaacgtatc tggaggcgaa atcatgttga ttccgtggca agacctctcc cccgaaacgc
    13141 tggaaaattt aattgaaagc tttgtgttac gtgaaggcac cgattatggt gaacatgagc
    13201 gtacacttga acagaaagtc gccgacgtca aacgccagct acagtgcgga gaagcggtgc
    13261 tggtatggtc ggagctgcac gaaacggtca atattatgcc gcgcagtcag tttcgcgaat
    13321 aacctgcaac cgtcggcgga cgagataata atgatagcta agtcatggag ttactatgtc
    13381 tgccaaacat ccggtcattg cggtaacagg atccagcggc gcggggacca ccaccaccag
    13441 cctcgcgttt cgtaaaatat tcgcgcagtt aaatctgcat gcagctgagg tggaaggcga
    13501 cagttttcac cgttacaccc gcccggaaat ggacatggcg atccgcaaag cgcgcgacgc
    13561 cgggcggcat atcagctact tcggccccga agcgaacgac ttcggcctgc tggaacaaac
    13621 cttcattgaa tacggtcaga gcggcaaagg gaaatctcgc aaatatctgc atacctacga
    13681 cgaagccgta ccgtggaatc aggtaccggg aacattcacc ccctggcaac ctttaccaga
    13741 acccactgat gtactgtttt atgaaggttt acacggcggc gtagtcacgc cacagcataa
    13801 cgttgcgcag catgtggact tactggtcgg cgtagtgcct atcgttaacc ttgagtggat
    13861 tcaaaaactg atccgcgaca ccagcgagcg cgggcactca cgagaagcag tgatggactc
    13921 agtagtgcgt tcaatggaag actatatcaa ctacatcaca ccgcagtttt cccgcaccca
    13981 tcttaacttc cagcgcgttc ccaccgtcga cacttcaaac ccgttcgcgg caaaaggtat
    14041 cccgtcgctg gatgaaagct ttgtggtgat ccattttcgt aatctggaag ggatcgattt
    14101 cccctggctg ctggcgatgt tgcaaggctc attcatttcc cacatcaata cgttagtggt
    14161 accgggcggc aaaatgggtc tggcaatgga attaattatg ctgccgctgg tgcaacgatt
    14221 gatggaagga aagaaaatcg agtaactctg ctattacgcc ggataaaata ctatccggct
    14281 tcacaacggg atagttaaat cacgcggcaa ccacttcata cgagtgagta atattcaccg
    14341 ctttttccag catcaacgcc actgaacaat atttctcggc agagagatca accgcacgcg
    14401 caaccgctgc gtctttcagg tcgcgaccgg tgacgataaa atgcagatta atgtgcgtaa
    14461 acaggcgtgg tgcctcttcg cggcgttcag aggtcaattt tacttcacaa tcgaccacat
    14521 cctgacgccc tttttgcagg atcgaaacca catcgatggc act
//
LOCUS       DAJTMC010000100        14222 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.100, whole genome shotgun sequence.
ACCESSION   DAJTMC010000100 DAJTMC010000000
VERSION     DAJTMC010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14222
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.100"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            505..1329
                     /gene="gadX"
                     /locus_tag="OO130_002711"
     CDS             505..1329
                     /gene="gadX"
                     /locus_tag="OO130_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HCR5366058.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTDRSAEGIRL"
     gene            1697..2425
                     /gene="gadW"
                     /locus_tag="OO130_002712"
     CDS             1697..2425
                     /gene="gadW"
                     /locus_tag="OO130_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HCR5366059.1"
                     /translation="MTHVCSVILIRRSFDIYHEQHKISLHNESIVLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSLGTFS"
     gene            complement(2788..5901)
                     /locus_tag="OO130_002713"
     CDS             complement(2788..5901)
                     /locus_tag="OO130_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HCR5366060.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIE
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKDNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPDQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIGAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(5926..7083)
                     /gene="mdtE"
                     /locus_tag="OO130_002714"
     CDS             complement(5926..7083)
                     /gene="mdtE"
                     /locus_tag="OO130_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HCR5366061.1"
                     /translation="MNRRRKLLIPLLFCGAMLTACDDKSAENAAAMTPEVGVVTLSPG
                     SVNVLSELPGRTVPYEVAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELN
                     SAKGSLAKALSTASNARITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAV
                     EQATINLQYANVTSPITGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQD
                     FLRMKEEVASGQIKQVQGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIF
                     PNPNGDLLPGMYVTALVDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIE
                     ASKAIGDQWVVTSGLQAGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            7143..7421
                     /locus_tag="OO130_002715"
     CDS             7143..7421
                     /locus_tag="OO130_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5366062.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNKVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(7422..7949)
                     /gene="gadE"
                     /locus_tag="OO130_002716"
     CDS             complement(7422..7949)
                     /gene="gadE"
                     /locus_tag="OO130_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HCR5366063.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITLTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(8748..9320)
                     /gene="hdeD"
                     /locus_tag="OO130_002717"
     CDS             complement(8748..9320)
                     /gene="hdeD"
                     /locus_tag="OO130_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HCR5366064.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            9575..9907
                     /gene="hdeA"
                     /locus_tag="OO130_002718"
     CDS             9575..9907
                     /gene="hdeA"
                     /locus_tag="OO130_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HCR5366065.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            10023..10349
                     /gene="hdeB"
                     /locus_tag="OO130_002719"
     CDS             10023..10349
                     /gene="hdeB"
                     /locus_tag="OO130_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HCR5366066.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            10413..11060
                     /locus_tag="OO130_002720"
                     /pseudo
     CDS             10413..11060
                     /locus_tag="OO130_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
     gene            complement(11102..11632)
                     /locus_tag="OO130_002721"
     CDS             complement(11102..11632)
                     /locus_tag="OO130_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5366067.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDGMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIIFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEAAVSLSRKQHQVLSCIANQMTTEDILEKLKITLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(11788..12354)
                     /gene="slp"
                     /locus_tag="OO130_002722"
     CDS             complement(11788..12354)
                     /gene="slp"
                     /locus_tag="OO130_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HCR5366068.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
     gene            complement(12709..13826)
                     /locus_tag="OO130_002723"
                     /pseudo
     CDS             complement(12709..13826)
                     /locus_tag="OO130_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312403.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         3946 a         3019 c         3155 g         4102 t
ORIGIN      
        1 attatgtgtc gtcgcggctt cgaaatggac tttgctgaac tgttgctgga agactacaaa
       61 gcctccctga aatatctcag cgatcacccg aaactgcagg gtattgccca gcagaacagc
      121 tttaaacaca cctgataaca taacgttgta aaaaccgaat gcccagcctt taaaaaaaca
      181 gctgggcatt cggttgctta ttaatcgcaa taatatattg gctgtctatt catcgtgttg
      241 ataagatata tgttatatga atgtttatta aatgtagcga ttgcctttaa tgtcacgaaa
      301 agggattatt tgcttgctat taatttcact gcgttcttaa acgcgttttt gtattataaa
      361 tccagtcatc ctgcccgtag aacatcacac attatcatcc tgttctcccg cttcgtttaa
      421 atttatttat caatcaattt gacttaagag ggcggcgtgc tacattaatt aacagtaata
      481 tgtttatgta atattaagtc aactatgcaa tcattacatg ggaattgtct aattgcgtac
      541 gcaagacata aatatattct caccatggtt aatggtgaat atcgctattt taatggtggt
      601 gacctggttt ttgcggatgc aagccaaatt cgagtagata agtgtgttga aaattttgta
      661 ttagtatcaa gggatacgct ttcattattt ctgccgatgc tcaaggagga ggcattaaat
      721 cttcatgcac ataaaaaaat ttcttcatta ctcgttcatc actgtagcag agatattcct
      781 gtttttcagg aagttgcgca actatcgcag aataagaatc ttcgctatgc agaaatgcta
      841 cgtaaaagag cattaatctt tgcgttgtta tctgtttttc ttgaggatga gcactttata
      901 ccgctgcttt tgaacgtttt gcaaccgaac atgcgaacaa gagtttgtac ggttatcaat
      961 aataatatcg cccatgagtg gacactagcc cgaatcgcca gcgagctgtt gatgagtcca
     1021 agcctgttaa agaaaaaatt gcgcgaagaa gagacatcat attcacagtt gcttactgag
     1081 tgtagaatgc aacgtgcttt gcaacttatt gttatacatg gcttttcaat taagcgagtc
     1141 gcagtgtcct gtggatatca cagcgtgtcg tatttcattt acgtctttcg aaattattat
     1201 gggatgacgc ccacagagta tcaggagcga tcggcgcagg gattgccgaa ccgtgactcg
     1261 gcggcaagta ttgttgcgca aggaaatttt tacggcactg accgttctgc ggaaggaata
     1321 agattataga attttactca gacataaaaa aaacctggca taggggaccg ggaagaggat
     1381 agtctgccgt ctccagacta ataaaccgct ataacactcc ctgttggcac gggaaacttt
     1441 gtgctctcag taagttaaat ataactttta ctggaaataa gatcagccat ttttttataa
     1501 acataagcta tacgctgtgc gaaaatatat tcgtgctgca tttacttatt atcaattaac
     1561 tgttatgcaa aactactttg tggataaatt ttggtcctac caaatctggc agtttttgcg
     1621 ctaagaaaca gtctggcaac atttcattag tatactgaaa ttgaaataat cgcagtatga
     1681 aatataaggg ataatcatga ctcatgtctg ctcggtgatc ctcattcgtc gttcattcga
     1741 tatttatcat gaacagcata aaatatcgct gcataacgag agtatcgtgc tgctggagaa
     1801 aaatttggca gacgattttg cgttttgttc accggatacg cgacgactgg atatcgatga
     1861 gctgacagtt tgccattact tacaaaatat tcgtcagcta ccacgcaatt tagggttaca
     1921 tagcaaagac cgtctattaa ttaaccagtc accccccatg ccgctggtga cggcgatttt
     1981 tgatagcttc aatgaatccg gggtaaattc accgatactg agcaatatgc tctacctttc
     2041 ctgtttatcg atgttttctc ataagaaaga actgatcccc ttacttttca atagcatcag
     2101 cactgtttca ggaaaagttg aacgccttat tagctttgat atcgccaaac gttggtatct
     2161 gcgcgatatc gcggaaagaa tgtataccag cgagagtctc atcaaaaaaa agttgcagga
     2221 tgaaaatacc tgtttcagta aaatattact cgcttccagg atgtcgatgg ccagacgatt
     2281 actcgagtta cgtcaaattc ctctgcatac tattgcagaa aaatgtggct atagcagtac
     2341 gtcgtacttt ataaacacat ttcgacaata ttatggtgta acgccacatc agtttgcgca
     2401 acattcgcta ggtacctttt cctgacatat tttgcatttg aatattggtc aggatctcac
     2461 acctgcttca tgtgaaactc ttccctgatg atttctgccg ggctaccggc tagttctctt
     2521 tcgcagtttg ccaggcggta taggtatttt caggaggaaa agactcggca tgtttgggat
     2581 tattaagctg acaattcata cgattacggg aatgtgggtc agtattgtgc tgttcaaatt
     2641 gatgacgaat ggctggagtg gtttctactt tcagtgctgt gtactaagcc tggtattcct
     2701 gacggttagc tggttgttga gtggtgaatg gttagcagga aagagtaagg ctgaaccttc
     2761 acgttcaacc ttactctcat ttacacgtta cgctttttta aagcgggcaa agagatgttc
     2821 cactacaacg aaaaagaccg gaacgaagta aattgccagc actgttgcgg caaacatccc
     2881 gcccatcacg ccggtaccta ccgcgttttg cgcgccagaa ccggcaccat gactgataac
     2941 cagcggcagc acgccgagaa taaaggccag agaggtcatc aggattgggc gtaaacgcat
     3001 ccgcgccgct tcgatgattg cccctatcgg cgttttccct tctttctgca tcatctcaac
     3061 ggcaaattcg acaatcagga tggcgttctt ggcggaaagc ccgatggtgg tcagcaaacc
     3121 aacctggaag tagacgtcat tacttaagcc gcgcagatcg gtggccagta atgcgccaac
     3181 gacgcctaac ggaacaacca acatcaccga gaacggaatt gaccagctct catagagtgc
     3241 ggcgagggcg aggaacacca cgaccagtga aatggcatac agtgcaggag cctggtttga
     3301 ggataacgct tcctgatacg acagtccggt ccatgagtag ccgacgcctg ccggaagttt
     3361 agcgaccagg tctgccataa atttcatagc gtcaccggta cttttcccgg ccgccgcttc
     3421 acctaaaatc tccattgacg ggatgccgtt gtagcgttcc aatcgcggtg aaccataggt
     3481 ccattcggta gacgagtagg cagaaagcgg tgccatcgtg ccagaggcgt tgcgtacata
     3541 ccattggttg atgttatccg gcaacatacg gaacggcgtg cctgcctgga catacacttt
     3601 tttcacccgc ccctggttga ggaagtcgtt cacgtagctg ctgccgaagg cggtggaaat
     3661 tgtctggttg atatcagaca gcgccacacc cattgcctca gctttcgcgg cgttgacgtt
     3721 cactttgaac atcggcgtat cttccaggcc gttcgggcgt accccggtga cctggtcagg
     3781 cgattgcgct gccagtgata acagctcgtt tcgtgcctgg gttagttttt cgtgccccag
     3841 gttaccgttg tccagcagtt ccatatcaaa acctgacgcg gtacccagtt cagccaccgc
     3901 gggtaagttg aacgggaaga cgacggcttt attgatactg cttaacgcaa tcattgcccg
     3961 ctgaatgatc gcggtaaccg agttttcctc accgacacgt tcagaccacg gcttgagact
     4021 gataaacgcc aggccgttgt tttgcccctg gccgctgaag ccaaagccgc caacggtaaa
     4081 caccgactga acattatctt tctcttttgt aagataataa tccgtcacct gttgcagcac
     4141 tttcgtggtg ttaaccatgg tggcaccgga aggtaactgc gcggtggtca taaatacccc
     4201 ctgatcctct tctggtaaga aagaggtcgg tgtacgcagg aacagcaccg ccatcccggc
     4261 gcaaatcagc aggtagacca ccatgtagcg accggtacaa cgcaacagcg agcgggtgct
     4321 atcggtatag tgctgagttg atttttcaaa cagcgtgttg aagcgtgcga acagggcgtt
     4381 aggtttgtga ccgccttccg gcgcggcttt cagaatggtg gcgcacaggg caggggtcag
     4441 gctcattgcc acaaatactg aaagcagcat ggaggagatc agcgtgatgg agaactggcg
     4501 gtagatctcc ccggttgcgc cgctcataaa ggccatcggc ataaacactg cggaaagaac
     4561 aacagcaata ccgaccagcg cacgttggat ctgccccatc gatttatgcg tcgcttcctt
     4621 cggtggtagc ttatcttccg caatgacacg ctcgacgttc tccaccacca cgatggcgtc
     4681 atccaccagt aaccctatcg ccagcaccat cccgaacata gttaacgtgt tgatggtgaa
     4741 accgaccgcc gacaagatcg caaacgtccc gagaataacc accggtacgg caatcgtcgg
     4801 gatgattgtg gcacggaaat tctgcaaaaa cagatacatg accaggaaga cgaggatgat
     4861 agcctcaacc agtgttttaa aaacttcctg aatagaaatt tcgataaacg gcgtggtgtc
     4921 gtaaggataa accgtcttca gacttgcggg gaaataggct gataagcggt tcagctcctc
     4981 tttgactgcc cgcgaggtat ccagggcgtt tgctccggta gccagtttga tggcgatccc
     5041 ggcagcaggt ttgccgttat agcgcgccac ggtggaataa tcttccgctc caagttcgac
     5101 gcgggcgaca tcacgcagca gcacttgcga accatcttgc tgaactttca acaggatttt
     5161 gccaaattct tccggcgttt gcagacgcgt ctgcacaatg atcgaggcgt ttagttgctg
     5221 gtctgccgcc tgtggcatgc cacccagttg accaccggaa atctggttgt tttgcacctt
     5281 aatctgggaa ataacatcgg aaggtaccag attgtattta ttgagttttt gcgggtccag
     5341 ccagatacgc atggcgtact cggaaccaaa gagctgtacg ctaccaacgc ccgcggtacg
     5401 gcttagcggg tctttgatat tagacgctac atagtccgcg atatcgtact ggttgaggct
     5461 gccgttatca gaaataaacg ccgctaccat cagaatatta ctgctcgact tatcaacgct
     5521 aatcccctgc tgctgcaccg cttcaggtaa tgaaggcata gcgagttgca gtttattttg
     5581 cacctgaacc tgtgcgatat caggagatgt cccagtctcg aaggtcagag tgatagaggc
     5641 attacccgcc gcatcactgg ttgaagacat gtacatcagg ccatcaagcc cattcatatt
     5701 ttgctcaatc acctgagtga ccgagtcttc taccgtttgc gcatcggcac ctggataggt
     5761 agcgctgacg gtaatggtcg gtggcgcaat ctgcggatac tgcgcaaccg gtaagttcat
     5821 gatcgccaga ccacctgcaa gcatcataat aatggcaagt acccaggcaa aaaccgggcg
     5881 atcaataaaa tagttagcca tgaaagtccc cttaagcctg caacgttatt gtttcgattc
     5941 ggtgctggcg ttttcctggc tggaggaaat tgctcgtgct ttgatacccg gacgaatgcg
     6001 ttgcaaaccg gaaacgatca cccgatcgcc agcctgcaag ccagaggtga caacccactg
     6061 gtcgccgatg gctttgctgg cttcaatttc gcgtagctgc acaacatcgt ctttatccag
     6121 aatgagcgcc gttgctttac cctgggcgtt gtgggtgacg ccttcctgcg gcaccagtaa
     6181 tacattctgg cggctacctt catccactaa tgccgtgacg tacatgccag gcagcaagtc
     6241 accatttggg ttggggaaaa tcgcccgtaa cgtcacggag cccgtggttt catccaccgt
     6301 cgggtcggag aatttcagtg tgccggtctg gctgtagcgt ttaccatttt ccagattgag
     6361 ctgtactggc gtactgccct gaacctgttt gatttgccca ctggcgacct cttctttcat
     6421 gcgcaagaaa tcctgcaccg actgcgtgag atcgacataa atcgggtcca gacgttgtac
     6481 ggtaaccagc gaatctgcct gattagcggt aacgagtgcg ccgacggtca ccgacgattt
     6541 cccgctgacg cccgtaatcg gcgaggtgac attcgcgtat tgcagattga tcgtcgcctg
     6601 ttcaacagcc gctttggcga cggtgacatt ggcttctgct tcattcagct gggtgcgcgc
     6661 ggtgtcgtaa tcctgacggc taacgtagtt ggtcttcagc aacgatgcct ggcggttaaa
     6721 ggtgatgcgg gcattgctgg cggtagagag cgctttcgcc agcgagcctt tggcggagtt
     6781 tagctcggcc tgtaaaggtg caggatcaat ctgatacagc gaatcgccct ggttcacttt
     6841 atcgccttcg ataaagttgc gtttaatgat aataccgccc acctggggac gtatctcggc
     6901 aacttcataa ggaacggttc taccgggcaa ttcgctcaac acattgaccg aaccggggga
     6961 gagtgtgacg acaccgacct caggcgtcat ggcggcggcg ttttccgccg atttgtcatc
     7021 gcaggcggtg agcatcgcgc cgcagaataa caacggtatt aacagctttc ttcttctgtt
     7081 cattttagtc cctgaaaatt cttgagaaat aacggttctt taaattgcgc gcgaggagag
     7141 atatgaggga tttacagacc agcggcatag tggggttgtc agccagtaag gtgggataca
     7201 ggcacagtga tcgacatggt gaggtcaacg acaaaaaaaa tgttgccgtt ctgccaacag
     7261 ttcctgccag cattcgggca acgaagggtt ctactggtgg atacacatac caggggaata
     7321 aagtcgtttt agcctctcct ttagtaatca ccgggggtaa tgaaatcagc atctgcatcc
     7381 ctcgtcatgc cagccatcaa tttcagttgc ttatgtcctg actaaaaata agatgtgata
     7441 cccagggtga cgatgtcgct catacgtctt agtttcaact tgtacataat gttgaatttc
     7501 tgaatcttta atgtttgttg cgaaatattg agcgtcaacg tgatattgct ttcattttta
     7561 tttttcagaa tcagttgcat aattttgaac tgattatgtg aaaaacataa ttgtgaattc
     7621 ttatggggca agtgtttacc ataagtaatc tcaagaagat ttttgatact ttctttgcgg
     7681 ctaacaaaat aaataggcgc tttagctttt agtttactga tgtgataagg tgccaaaaca
     7741 ataattctct cggcatctaa tttctccaga aatttaatcg cttcttcatc aaggatatga
     7801 ttgtgatagg tatcaatgat aaccttgaag ggtttattgc ctactttttt gatctctgac
     7861 agggaattga tttttatcat ttcgtgatta tctttcaact gccaaaagcc ctgtaaaaga
     7921 aaagaatctt tcgtcatgag aaaaatcata acttgctcct tagccgttat cgtttcgaac
     7981 atgtcatcct tttcattatt tacatccttg tccgaatcgt tgttcaatat agtaaacgcc
     8041 tataagaact ttacccttcg tgaactaata cgtctattaa cactaatcaa tacttcagat
     8101 ttatccatca cgcactccat atattgatcc taagtaagat tcctggttgt tatcagcttg
     8161 taaatagtaa acaaaaagtt aacgtttgcg gattactgcc caagaataag ttatcaataa
     8221 ccagtttgtg atctctgaag aatattacta aagttaaaat gtaatccgat ttaaatatcg
     8281 agtctccttg tttcgactta agctggcaat tggattgcca gctttctttt aacgcattga
     8341 aattacctga gtaaataacg cattcatgtc ttcaggtttt tatatagcgt ctggagacac
     8401 ggatatttat gcaatgtaat caacgccagt tgcaaaaaaa tgaacccgga ttacttattt
     8461 aaccgcccat agcatcaacg cttacggaac tctttgttca ctttcccaca gcacttttaa
     8521 aacattaaac ctgactacgg aaaatatcag ccatgataat gtttgaaatg gctaatttgc
     8581 catggagtga aaaaaattag atgaaattca gtaggttgaa ataatcacta gcaggtaatt
     8641 atttcaatga tagtgcgcaa ttgatctaca acactgcgta gcggagagag tattaatcgg
     8701 atcatagtca catcaagtga ctatgatccg ggtgacaacc ggggtaatta ttgctgctta
     8761 acgaacaaac tggcgaagct gaacaggctg gcggcgctaa atatcagttc aattcccacc
     8821 agtgtggaaa ccagcgttac agacaccatc ggcgttgcac caaggaatat ccaggcaatg
     8881 acgatatcca gcacaccaat aacgagctgt agccagctgc ctttcattga acgctgacga
     8941 taccaactca tcaggcgaat aacccctgca acacagaaca aaccggcaat aaatgccgca
     9001 atggcaaaaa tgcccagctc cggtgcgcgg atgaagaaat agccgatcaa taaataggcg
     9061 actgcgacga ggaaaccgga taataccggc cagaaattat gactgcggtt gctgaataac
     9121 ccgacaataa gcgcaatacc cgagcagatt aataatgcac ccactactgt gcttaaaata
     9181 tcgccagaga cgaacgggaa actgatacac agcaacccga cgataaacag cagcacggca
     9241 ataaactgga ttgctctgcg atgtttttta agcatctcca gatcaaactt caaaattgtt
     9301 gccttatcta tatataacat agaaccaccc tataaaatta agaagaaaat cccctgctat
     9361 caatctatgc caaaaacgcg tctaagaatg cagtcgattt aataaaaatt tcctaattgc
     9421 agtatctgat gcatctgtaa ctcattgtat tgaaataaaa atatctgatt ttgatatttt
     9481 ccatcaacat gacatataca gaaaaccagg ttataacctc agtgtcgaaa ttgatttgtg
     9541 acggctcttt cactttatag ttgaggatat tacgatgaaa aaagtattag gcgttattct
     9601 tggtggtctg cttcttctgc cagttgtgag caatgcagcg gatgcgcaaa aagcagctga
     9661 taacaaaaaa ccggtcaact cctggacctg tgaagatttc ctggctgtgg acgaatcctt
     9721 ccagccaact gcagttggtt ttgctgaagc gctgaacaac aaagataaac cagaagatgc
     9781 ggttttagat gttcagggta ttgcaaccgt aaccccagct atcgttcagg cttgtactca
     9841 ggataaacaa gccaacttta aagataaagt taaaggcgaa tgggacaaaa ttaagaaaga
     9901 tatgtaattc cgggaatgcg ttacatcgta cttccttgca tattgaacag gccggaatat
     9961 cttctttaaa agcagctatt cctcctgttc atatataatc tatatattga atgggttaca
    10021 aaatgaatat ttcatctctc cgtaaagcgt ttatttttat gggcgctgta gcggctttgt
    10081 cactggtgaa cgcacaatct gcgctggcag ccaatgaatc cgctaaagat atgacctgcc
    10141 aggaatttat tgatctgaat ccaaaagcaa tgaccccggt tgcatggtgg atgctgcatg
    10201 aagaaacagt atataaaggt ggcgataccg ttactttaaa tgaaaccgat ctcactcaaa
    10261 ttcctaaagt gatcgaatac tgtaagaaaa acccgcagaa aaatttgtat accttcaaaa
    10321 atcaagcatc taatgacttg ccgaattaat gaggtacaag taaaaaggag tagcaagttg
    10381 agccatcttg ctgctccttt ttgcattttt atatgacagc agaatttatt atacgtctta
    10441 tactcgcggc aattgcctgt ggcgctattg gcatggaaag gcaaatgtga ggcaaaggag
    10501 cagggttacg cacacatgta ttaattggca tgggaagcgc cctgtttatg attgtttcga
    10561 aatatggttt tgctgacgtg ctgtctttag atcacgtcgg actcgacccc agccgtattg
    10621 ctgctcaggt ggtgacgggc gtcgggttta tcggtgcagg taacattctc gttcgtaacc
    10681 aaaatattgt aggtttgacg acggcagcgg atatctggat gaccgccgct ataggtatgg
    10741 ttattggcag tggtatgtac gaattgggta tgtatggttc agtgatgacc ttgctggtgc
    10801 tggaagtctt ccatcaatta accttccgcc tgatgaacaa aaattaccat ttacagctga
    10861 cattagtgaa cggtaatacg gtatcaatgc tcgactggtt taagcagcaa aaaataaaaa
    10921 cggatttagt ttcgttgcag gaaaatgaag atcatgaagt ggtcgccatt gatatccagc
    10981 ttcatgccac aacgtcgata gaagatctgt tgcgattatt aaaaggtatg gcaggggtga
    11041 aaggcgtttc aattagctaa tctgtgtggc agcgtagtca gactcaccgt aagcctgaaa
    11101 ttcacaccag ataatcaata tgctgatggc gtaccagctc attgatccgc ttaagattga
    11161 ggatcatcat gatattgtgt ttatggcagt agaacgtttt tagcgttatt ttcagtttct
    11221 ccagaatatc ctccgttgtc atttgattcg caatgcagct cagaacctga tgttgcttgc
    11281 gcgaaagtga cacagccgct tcaggcacag agcagtaact gccattgatg agtgcgacaa
    11341 aatcgatgat agtgatagtc cgcggaacaa aggtcaccgg cactttcccc aggcagcgtt
    11401 taatattaaa gggggagaaa ataatcacat ggacaggttt taatcgttca atttgagtga
    11461 gaaaattaga atgaaataca ttattcatta atgtatcaat aatgacaaag gcattctgat
    11521 gcaacattcc gtcgatctct tcttcgttct gtatatgaac gacattacct ttgctcaaaa
    11581 tgtttttcat cgcggtgaag aacatcgtat ccctggtaat tataagaaac ataaccgact
    11641 cctggtgcgg acattaattt tataatcagt ttgccaaagg taattaacag gacagggctt
    11701 ataataataa tagttagctt gctgtgtatt aattttatgt aatgcacgat gcagatgctg
    11761 agaaagcatc tgcatctttc ggtggtgtta tttgaccagc tcaggtgtta cctgactcac
    11821 cgcattggtg tagtaaggcg caccccagcc cggttccggc cagaatgcac catagccgta
    11881 atcccatagg ttataggtgg tattaaccac ttctctcaaa tgccacacct ggatgccctg
    11941 catattcact tccaggaagt tatacgggac tttattgata aagccaggtt gttcaccctg
    12001 aatggtaccg aggatggtaa caaagtgatt acgatagttc actgggtcaa ggaagccgct
    12061 ttgtctggcg agcagtcggc cctgatagtt ggcttcaata tcaggtttcg catagctatc
    12121 caacggtaat acagcgattt ctaacaacgt atccgttttg ccattgataa cgttgataac
    12181 cttcccacca aagcgcgctt gttgaccaac atataacccc ggctggttat gaacagcaac
    12241 aaaacttttt tgaatatcag gttggttatt gcctttgata ttttgcggaa ttgaactaca
    12301 tgcggcaagc aaaaatgaaa ggctgaggat gagtgcacct tttgtcatgt tcatgttact
    12361 atccttatca acaaatatta aaaccataat agatatttct ttatagtttt catctgattc
    12421 tgaggcgata tgcctgatag taacgtaaac ctgtctgatg tttatatccc ttttgtttag
    12481 ttcaattcca ttgattttgg ggcgtggcat aagtatccat cgccccataa tataatcctt
    12541 tgttttaaaa ttacataaaa tctattttta ttcaataatt ataaagtgct aataatcaat
    12601 attatggtat tatattctga taatgatttg ataagcgaat tcttttgcca gatggtattt
    12661 ctatttccac cccataattt atatcttttt cattaggaag tggcctcttt aaataaggcg
    12721 tgtgtgacat tttttttggg ttaagcgtct cgaattgaaa tacttcgaac tgatattcag
    12781 acatttctgc ccatgtttga attttattgt atcgagagag tgtcgtagag gctttaactt
    12841 gcttataaca ttgtttttgg aattcttgct gatattttaa tatgtcattg tatgaaagat
    12901 tggttatcct ggcctctaaa aatttaatta ttgtcgaagc aatttccgct tttgctcgta
    12961 aaaagttgtc attaatggtt aataatgttg aaaataaacc ttcgatttgt ttgtcaagat
    13021 attgtccatc tttatataat ttgggggggt agggtgttct ttatgtaaaa aatagtgtca
    13081 cctaaatttg cgtcagtgaa atttatatta agtagatctt cgaaataaaa ccttgacaag
    13141 gttagagcga acccggaaag gttctgtcct gtcatattga aatcaataaa gttggttccg
    13201 gcaaagactt tcatcctgtt tttattttca ctgtgctcaa tatataactt cgtcattttt
    13261 tgatacaggc tttgaatatc ttttatatta gttggtaagt cagctcgttg ttcagcaata
    13321 aaattagtat ctgaaaatat ggtttggaca ctgaatgtta tgggtattcc ctcttctacg
    13381 ctaattttag ttatgattgc cttgtcgggt ttaggattag cgacaacaat gattcgacat
    13441 tcagcacttt tgtttttttc atatctaatg ttattaatct gattaagtat tgattgtgtc
    13501 tgttgcgtaa gtggtgtcga acttatcgtt tcaatgttat taataagact tacaagttga
    13561 accacaactt ttgagtagtc tgcatttgta tatgtggtgt tcgtgtggca tgcattaatt
    13621 tgttggagtt gttgttgcgc taaatcaaat tgttcagggt tgtatccttc tccagagaat
    13681 gtggaattaa tgggttgttg ttcttcccaa ttatcacaag gccacttcgg tgtgtctggc
    13741 gttttaacat tatcggtatt tgttgtgcag ttataaatat tgccgtgata tgtatttatt
    13801 atacctgggg taaagtcaat tgacataaca ttccttattc atcatctgtt ttatgctaag
    13861 gtttttaaat tggataatta gaacaggaat ggattttctt ctacacacaa taaatataat
    13921 gcgttatatc ttcctgatat aattaccttt aaattccctt tcatttccac ccggtaatat
    13981 ttgtgtataa aaaaagcagt gatagtatcg taaaggaaaa cgttaacaac tatcaaaaag
    14041 cgctcatttt aaaaagttat tatgttctcc gatagtgtgt gaagtaatat ttttgtgatg
    14101 ttttagatgt gtaataatga accaggttaa agtgtattta aaaatgtgtt gtattagcga
    14161 ctgaaatctg ccttttaaaa aggaggaggg tactgagaca aagagtcatt gcttctccga
    14221 ta
//
LOCUS       DAJTMC010000101        14109 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.101, whole genome shotgun sequence.
ACCESSION   DAJTMC010000101 DAJTMC010000000
VERSION     DAJTMC010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14109
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.101"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            220..1167
                     /locus_tag="OO130_002724"
     CDS             220..1167
                     /locus_tag="OO130_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001361319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HCR5366069.1"
                     /translation="MKNVLVTVPSFSARCVSASKLLRENNFNLIIKNNVEHLLKSEST
                     ALRESICAVIAGKDCYQADTLSLLPGVKIISRFGTGIDNIDLRAAQQSGIVVNNAVGI
                     NSNAVAEFIIGLIFASMRNIPGSYHAMQNGYWGESHGYELQGKRIGMVGYGNIGKTLA
                     KRLSGFDVELLAYDKQPDYQVADKVGVQFVSIEDIFMQSHVIIVLLPFSPELENFISH
                     KYLSMMRNGALIINAARGKLLDEGALLQVIEERNVFAALDVFSSEPLAQFSPLLHAKN
                     IITTPHIAAATVESYQQTGTHVAQSIIDYFAGREIKNVL"
     gene            complement(1246..1322)
                     /locus_tag="OO130_002725"
     tRNA            complement(1246..1322)
                     /locus_tag="OO130_002725"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1286..1288),aa:Met,seq:cat)
     gene            complement(1355..1431)
                     /locus_tag="OO130_002726"
     tRNA            complement(1355..1431)
                     /locus_tag="OO130_002726"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1395..1397),aa:Met,seq:cat)
     gene            complement(1465..1541)
                     /locus_tag="OO130_002727"
     tRNA            complement(1465..1541)
                     /locus_tag="OO130_002727"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1505..1507),aa:Met,seq:cat)
     gene            1750..2847
                     /gene="mltA"
                     /locus_tag="OO130_002728"
     CDS             1750..2847
                     /gene="mltA"
                     /locus_tag="OO130_002728"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HCR5366070.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKSTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LGDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            2924..3730
                     /gene="tcdA"
                     /locus_tag="OO130_002729"
     CDS             2924..3730
                     /gene="tcdA"
                     /locus_tag="OO130_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HCR5366071.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSIGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(3781..4224)
                     /gene="csdE"
                     /locus_tag="OO130_002730"
     CDS             complement(3781..4224)
                     /gene="csdE"
                     /locus_tag="OO130_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HCR5366072.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLTQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGDSEGRIVLGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRGQLSASRSQGLNALSEAIIAAAKQV"
     gene            complement(4224..5429)
                     /gene="csdA"
                     /locus_tag="OO130_002731"
     CDS             complement(4224..5429)
                     /gene="csdA"
                     /locus_tag="OO130_002731"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HCR5366073.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALSQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAENWSHSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            5621..5848
                     /locus_tag="OO130_002732"
     CDS             5621..5848
                     /locus_tag="OO130_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HCR5366074.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            6199..7116
                     /gene="gcvA"
                     /locus_tag="OO130_002733"
     CDS             6199..7116
                     /gene="gcvA"
                     /locus_tag="OO130_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HCR5366075.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            7135..7530
                     /locus_tag="OO130_002734"
     CDS             7135..7530
                     /locus_tag="OO130_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HCR5366076.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            7523..8623
                     /gene="rlmM"
                     /locus_tag="OO130_002735"
     CDS             7523..8623
                     /gene="rlmM"
                     /locus_tag="OO130_002735"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HCR5366077.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENSGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(8667..9398)
                     /gene="fucR"
                     /locus_tag="OO130_002736"
     CDS             complement(8667..9398)
                     /gene="fucR"
                     /locus_tag="OO130_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HCR5366078.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICYELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(9456..9878)
                     /gene="fucU"
                     /locus_tag="OO130_002737"
     CDS             complement(9456..9878)
                     /gene="fucU"
                     /locus_tag="OO130_002737"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HCR5366079.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            complement(9880..11298)
                     /gene="fucK"
                     /locus_tag="OO130_002738"
     CDS             complement(9880..11298)
                     /gene="fucK"
                     /locus_tag="OO130_002738"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HCR5366080.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTECHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIEQLISAQRLQAISGVGAFSFNTLYKLVWLKENHPQLLERTHAWFFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGADQNEPVLSSGTWEILIVRSAQVDT
                     SLLSQYAGSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     SADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            complement(11295..13040)
                     /gene="fucI"
                     /locus_tag="OO130_002739"
     CDS             complement(11295..13040)
                     /gene="fucI"
                     /locus_tag="OO130_002739"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HCR5366081.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEVLQERL"
     gene            complement(13073..>14109)
                     /gene="fucP"
                     /locus_tag="OO130_002740"
     CDS             complement(13073..>14109)
                     /gene="fucP"
                     /locus_tag="OO130_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HCR5366082.1"
                     /translation="ITGLFLYALGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAA
                     NPFVTVLGPESSGHFRLNLAQTFNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQ
                     LSAYKHSLVLSVQTPYMIIVAIVLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRL
                     ARIRHWRWAVLAQFCYVGAQTACWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGR
                     FTGTWLISRFAPHKVLAAYALIAMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFS
                     LGIKNLGQDTKYGSSFIVMTIIGGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIF
                     ARFRSQTATN"
BASE COUNT         3406 a         3469 c         3708 g         3526 t
ORIGIN      
        1 aggtttagca ccgtcatgct cgtctactgt tgttctggtt gttggtgatg cggtggcgct
       61 tgcactctct gaattgaaaa aatttactcg tgctgatttc ggcttatatc atccaggagg
      121 tgcactcggc attaaagcaa attcataatt tcaaagtata aatacagtga ccggtcacgc
      181 catcttttgg ggtggcgttc attgaagtga gtgtcagcta tgaaaaatgt tctggtcacc
      241 gtccccagct tttcagcacg ctgtgtttcc gccagcaagt tgctaagaga gaataatttt
      301 aatcttatta ttaagaataa tgtggagcat ctgcttaaat cagaatccac tgcattacga
      361 gaatctattt gcgcggtcat cgccggaaaa gattgttatc aggcggacac gctatcattg
      421 ctgcctggtg tgaaaataat atcgagattc gggacgggga tagataatat tgatcttcgt
      481 gcagcacaac aatccggtat tgtagtcaat aatgcggttg ggattaattc aaatgctgtc
      541 gcggaattta tcattggact tatcttcgcg agcatgagaa atattcccgg cagctatcat
      601 gcgatgcaaa atggctactg gggtgagtcg catggctatg aattacaagg caaacggatt
      661 ggcatggttg gctacggcaa tataggtaaa accctggcaa aaaggttatc cggttttgat
      721 gttgagcttt tagcttatga caaacaaccc gattatcagg ttgctgacaa agttggagtt
      781 cagtttgtat caattgaaga tatctttatg cagtcgcatg tcatcattgt tcttttgcct
      841 ttttctcctg agctggagaa cttcattagt cataaatatt tgtcgatgat gcgcaatggt
      901 gctctaatca tcaatgccgc aaggggaaag ttgctggatg agggcgcttt actacaggtg
      961 attgaagaac gaaatgtgtt tgcggcgctg gatgtgttta gctctgaacc gttggcgcaa
     1021 tttagcccac tgctacatgc caaaaatatc attacaacgc cgcatattgc ggccgccacc
     1081 gttgagtcat accagcaaac cggtacacac gttgcccagt cgataattga ttacttcgca
     1141 ggaagggaga taaaaaacgt gttgtgagtt cagcgaacga agtttttaaa tacaaaaaaa
     1201 ccgcaatttg tggaacacat tgcggtttat cagcattcta agtattggtt gcgggggccg
     1261 gatttgaacc gacgaccttc gggttatgag cccgacgagc taccaggctg ctccaccccg
     1321 cgtcaccgta ctgctttatt tcatcaaatt tgattggttg cgggggccgg atttgaaccg
     1381 acgaccttcg ggttatgagc ccgacgagct accaggctgc tccaccccgc gtcaccgtac
     1441 tgctttactt catcaaattt taattggttg cgggggccgg atttgaaccg acgaccttcg
     1501 ggttatgagc ccgacgagct accaggctgc tccaccccgc gtccgtggat gcgcactata
     1561 ctccgctcgc gttttcatgc aacctttttt ttaccccgat cacggattta gcatgatttt
     1621 gaacaaaaac aaaccactaa agcctatttt ttgtgcaaaa tttgcgtcag ggagaggctg
     1681 ctcatttcat taaacgcggc ggtttgttat cttcgttgcg ccttattttt taacctgaag
     1741 aagagaacaa tgaaaggacg ttgggtaaag taccttctta tgggcacggt tgtggcaatg
     1801 cttgccgcct gctcttccaa atcaaccgat cgcggacagc aatataaaga cgggaaattt
     1861 acccagcctt tctctctggt gaaccagcca gatgccgttg gcgcgccgat taacgccggt
     1921 gattttgccg agcaaattaa ccatatccgt aattcgtcac cgcgtctgta tggcaaccag
     1981 agtaatgttt ataacgcggt gcaagagtgg ctgcgcgcag gcggtgatac ccgcaatatg
     2041 cgccagttcg gcattgatgc ctggcagatg gaaggtgccg acaactatgg taacgtgcag
     2101 tttacaggct attacacgcc ggtaattcag gcgcgccata cccgccaggg cgagttccag
     2161 tatcctattt accgtatgcc gccaaaacgt ggtcgtctgc cgtctcgtgc ggagatctac
     2221 gcgggggcat tgggcgataa atacatcctc gcttacagca actctctgat ggataacttc
     2281 attatggatg tgcagggtag tgggtatatc gactttggtg atggcagtcc gcttaacttt
     2341 ttcagctatg cagggaaaaa cggtcatgcc tatcgcagca ttggtaaggt gctgatcgac
     2401 cgtggcgaag tgaaaaaaga agatatgtcg atgcaggcga ttcgtcattg gggcgaaaca
     2461 cacagtgaag ccgaggttcg cgagctgctg gaacagaacc cgtctttcgt cttctttaaa
     2521 ccgcaatctt ttgcaccggt gaaaggggca agtgcggtgc cgctggttgg ccgtgcttct
     2581 gttgcctccg atcgttcaat tattccgcca ggtactacct tgctggcaga agtgccgctg
     2641 ctggataata acggcaaatt taacggtcag tacgaattgc gtctgatggt ggcgctggat
     2701 gtcggtggcg caatcaaagg ccaacacttc gatatctatc aagggatcgg gccggaagcc
     2761 ggacatcgcg caggttggta caaccactat ggacgtgtct gggtgctgaa aaccgccccg
     2821 ggcgcaggta acgtctttag cggctgatgt gttattctgt ataccgtttt tacggatacg
     2881 acagggtgag ggataacctc acccttttta attctgaggt gctatgtctg tggtaattag
     2941 tgatgcatgg cgtcagcgtt ttggtggcac agcgcgtctg tatggtgaaa aagcgttgca
     3001 actgtttgct gacgcgcata tttgtgtggt tggtatcggt ggtgtcggtt cctgggcggc
     3061 ggaagcgctg gcgcgcacgg ggattggcgc aatcacgctt atcgatatgg atgatgtgtg
     3121 cgtcaccaat accaatcggc aaattcacgc tctgcgcgat aacgtcgggc tggcaaaagc
     3181 ggaagtcatg gcggagcgca ttcgccagat taacccggag tgccgtgtaa cggtggtgga
     3241 tgatttcgtg acgccggata acgtagcgca atatatgagt gccggttatt cgtacgtgat
     3301 tgatgccatt gatagtgtac gacccaaagc ggcgctgatt gcttattgtc ggcgcaataa
     3361 aatcccgctg gtcacgaccg gtggcgcggg tgggcagatt gacccgacgc agattcaggt
     3421 taccgatctg gcgaaaacga ttcaggaccc gctggcggcg aagttgcgcg agcgcctgaa
     3481 aagcgatttt ggcgtagtga aaaacagtat aggtaagctc ggcgtggatt gcgtgttttc
     3541 tactgaagcg ctggtgtacc cgcagtcaga cggtacggtg tgtgcgatga aagccacggc
     3601 agaagggccg aagcggatgg attgtgcatc tggatttggc gcggcaacga tggtgaccgc
     3661 cacctttggg tttgttgcgg tttctcatgc gctgaagaag atgatggcga aagcagcgcg
     3721 tcagggttaa acaatcaggt cggatgcgac gcgaacgtct tatccgacct ggttctgtgc
     3781 ttaaacctgc ttcgccgcag cgataattgc ctcgcttaac gcattcaacc cctggctgcg
     3841 tgaggcgcta agctgcccac gcaatcccag ctcatcaaac aatgccagcg gcgactgtgc
     3901 ctgtaattcg gcggcggttt tcccctcaac ggcagtcaac aacaccgcca acaggccgag
     3961 cacaatgcgg ccttcgctgt cgccaaagaa gtgcatttta ccgttttcag ccacggtata
     4021 tcccagccag acgcggtttt cgcatccggc aatctcttta gcctgcgctt ttaactcgtc
     4081 tggcaaagcc ggaagctgtt tccccagcat gatcaactgg cgatatttat cttcccattg
     4141 cgtcaacggt gcgaaggtat tgcgtaacgt ttctgcggtt acggttgtgc cgaacggatg
     4201 tccggcgaat tgcgggtttg tcattaatcc accaataatt ccagcgcgcg gtcaacggca
     4261 ttcaccagcg catccacatc actctttgta ttatatggcg caaaagaggc gcgcagtgtg
     4321 ccggttacgc ctaattctgc cagtagcggc tgagcacaat gttgcccggc ccgcaaggca
     4381 ataccgtact ccgccagtag cgtcaccata tcgctgtgat gaacgccagc aaaatcaaag
     4441 gccagcaggc tggaatcctg gcagcggaat gaacgaaagc ctggacgttt cgccagcgca
     4501 tcttccgcca gcgttgctaa gctatggctc cagttttcgg cctggttgat atcgtaatct
     4561 gccagccatt ccagcgccgc gcttaatcct atgacaccag ccacatttgg cgttcctgct
     4621 tccagtttcc acggcgcaga ttgagtcgtg aagccgtcaa aactcacttc gtgaatcatt
     4681 ttgccgccac ccagccaggg cgacatcgct tccagcagtt ctgatttgcc atacagcacg
     4741 ccgatacccg tcgggccata cagtttgtga ccagagaaag catagaaatc gatatccagt
     4801 tgctgaacat ccgcggggaa atgcaccgcc ccctgagcac catcaaccat caccaccatc
     4861 cctgctgaat gagcaaaggt aatcgctcgc gccagatccg ggcaaccgcc agtaacgttc
     4921 gacatctgac tcaacgccag aatccgacta cggggagtaa tcagttctgg caacaaatcg
     4981 acatccggca gtcgctgcgc attaagcggc aatttcacca ctttggctcc agtttgttgg
     5041 gcgaccatca gccaggggac gaggttggcg tggtgttctg ccacgctgac aataatctca
     5101 tcgcccggtt gcagacgcgg acgcgcatag cattgtgcca ccatgttgat ggattcagtg
     5161 gtgccgcgcg tccagacgat agttttatca tccggtgcat tcagtaattg cgccactttc
     5221 tctcgtgcag cttcataacg cgcggtcagg cgttgggctt cggcaaactg gctgcgatgg
     5281 acgtttccgg cgctcaggct gtaaaactgt cgagtggctt caaccacggc ttcaggttta
     5341 agcgcggttg cggcgctgtc gagatagacg cccgcatcct gtagtgcggg aaactgggcg
     5401 cgaaactgcg cgggattaaa aacgttcatg gtactcctcg gcttgatgca ccgatcgtga
     5461 cgcaaaattc actttgatac aaggagagtg ctaaccatca gaattatctg gatatcctgg
     5521 cgaagccgcg taagtaacat atgctgaata gtgttaggcg ttaattttag cctgttaatc
     5581 aatactatta agatttatta atcgctttaa ggagaagatg atgaaaaaga ctgccgcaat
     5641 tatttctgcc tgtatgctga cttttgccct gagcgcctgt tccggttcga actatgtgat
     5701 gcacaccaat gacggacgta ccatcgtctc tgacggcaaa ccacagactg ataacgatac
     5761 cggtatgatt tcgtataaag acgctaatgg caacaaacag cagatcaacc gtactgacgt
     5821 gaaagagatg gtcgaactgg atcagtaatt cgcgatcgca aggtaaaaaa aagcaccgca
     5881 attaggcggt gctacattaa tcactatgga cagacagggt aaatgtacag gaagtgaaaa
     5941 aaggtagctt tgctaccatg gtctgaatcg cagaccaatt gcaaacacaa caacacaaca
     6001 tcacaaccgt aagccaaaag ttcaccagaa cacgcattcc gataaaactt ttcgttccgg
     6061 ctcaggaagt gccgccacta taggtatttg ctggtagaag ctcaacggac aatttataat
     6121 ggctcagatt aaaaaaacta ataggttaca tagtgtgatc taattgttaa attcatttaa
     6181 catcaaagtt taatagccat gtctaaacga ttaccaccgc taaatgcctt acgagttttt
     6241 gatgccgcag cacgccattt aagtttcact cgcgcagcag aagagctttt tgtgacccag
     6301 gccgcagtaa gtcatcaaat caagtctctt gaggattttt tggggctaaa actgttccgc
     6361 cgccgtaatc gttcactcct gctgaccgag gaagggcaaa gctatttcct cgatatcaaa
     6421 gagatatttt cgcaattaac cgaagcgacg cgtaaactcc aggcccgtag cgccaagggg
     6481 gcgttgacgg tcagtttact ccccagtttc gccattcatt ggttggttcc gcgactttcc
     6541 agctttaatt cagcttatcc gggaattgac gttcgaatcc aggcggttga tcgtcaggaa
     6601 gataagctgg cggatgatgt tgatgtggcg atattttatg gtcggggcaa ctggccgggg
     6661 ctacgggtgg aaaaactgta cgccgaatat ttattgccgg tgtgttcgcc gctactgctg
     6721 actggcgaaa aacccttgaa gacaccggaa gatctggcta aacatacgtt attacatgat
     6781 gcttcgcgcc gtgactggca gacatatacc cgacagttgg ggttaaatca tatcaacgtt
     6841 cagcaagggc caatttttag ccatagcgcc atggtgctgc aagcggctat ccacgggcag
     6901 ggagtggcgc tggcaaataa cgtgatggcg caatctgaaa tcgaggccgg acgtcttgtt
     6961 tgcccgttta atgatgttct ggtcagtaaa aacgcttttt atctggtttg tcatgacagc
     7021 caggcagaac tgggtaaaat agccgccttt cgccaatgga tcctggcgaa agccgctgct
     7081 gaacaagaaa aattccgctt tcgttatgaa caataattta cgtagggtac gaccatgacc
     7141 agccgtttta tgctgatttt cgccgccatt agcggcttca tttttgtggc tctgggcgct
     7201 tttggcgcgc atgtgttaag taaaaccatg ggagccgttg agatgggctg gatccagacc
     7261 ggcctcgaat accaggcgtt tcatacgctg gcgatcttag gtctggcggt ggcaatgcag
     7321 cgtcgcatca gtatctggtt ttactggagt agcgttttcc tcgcgttagg cacggtgttg
     7381 ttcagcggca gcctttattg cctggcgctg tcccatctgc gtttgtgggc gtttgtcact
     7441 ccggttggcg gcgtgagctt cctcgcgggc tgggcgttaa tgttagttgg tgctatccgt
     7501 ttaaagcgca agggcgtaag tcatgaataa ggttgtattg ctgtgccgtc cgggctttga
     7561 aaaagagtgc gccgcagaaa ttaccgataa agccggccag cgggaaattt tcggttttgc
     7621 ccgcgtgaaa gagaattcgg gttatgtcat ttatgaatgt tatcaacctg atgatggcga
     7681 taagttaatc cgtgagctgc cgttcagttc attaattttt gcccgccagt ggtttgtggt
     7741 gggggaactc ctgcagcatt tgccgccaga agatcgtatt acccccattg tcggcatgtt
     7801 acagggcgta gtagagaagg gcggtgaact gcgtgttgaa gttgccgata ccaacgaaag
     7861 caaagagttg ctgaaattct gccgtaaatt tactgttccg ctacgcgctg ccttgcgcga
     7921 tgccggggtg ctggcgaact atgaaacgcc gaagcgtccg gttgtgcatg tattctttat
     7981 tgcaccaggc tgctgctata ccggttactc atacagcaac aataattcgc cgttctatat
     8041 gggcattccg cgcctgaaat ttccggcaga ggcgccgagt cgttccacgc tcaaactgga
     8101 agaggcattt catgtgttta ttcctgcgga tgagtgggat gaacgcctgg cgaacgggat
     8161 gtgggcggtg gatttaggcg cttgccctgg cggctggacc taccaactgg tgaagcgcaa
     8221 catgtgggtt tattccgtcg acaacggccc gatggcgcaa agtctgatgg ataccgggca
     8281 ggtgacgtgg ctgcgggaag acggtttcaa attccgtccg acacgcagca atatctcctg
     8341 gatggtatgc gatatggttg aaaaaccggc taaagttgcg gcattgatgg cgcagtggct
     8401 ggttaatggc tggtgccgtg aaaccatttt caatctcaaa ctgccgatga aaaaacgcta
     8461 cgaagaagta tcacacaatc tggcgtatat tcaggcacag cttgatgaac atggcataaa
     8521 tgctcagatt caggcgcggc agttgtatca cgatcgcgaa gaagtgacgg tgcacgtccg
     8581 ccgcatctgg gctgcggtgg gtggtcgtcg cgacgagcga taacaggcaa ggccggggcg
     8641 tcgtcttcgg cctgctaccc tttccgtcag gctgttacca aagaagttgc aacctggcgc
     8701 tcatcagaaa taatgtgcgt tacctcatcc agatgcccga tgcgggcttc ccctgaacga
     8761 ttaaatttac ttttatcaat caataacaac gattgcgcgg cacgttttaa tagcatcgat
     8821 ttgtaatcag cgttgatcgc attggagtcc cacagtgcgc cgctgctatc gatcccttca
     8881 caagaaaaaa taaacagatc gatttccagc gatttaagtt gggaaatcag cgagggattg
     8941 acgtaacagc catatttgcg ctcaagtgtg ccgccggaac tgatcagttg aatgcgttcg
     9001 cgtttaccga gttcatagca aatcggatgg ctattggtga agacctgaat gttgatgtca
     9061 ggcaactggc gtgccagata ccagcaagtt gaactggcgt ctaaggctat caccatccct
     9121 tcttcaatcc acgcgagcgc ctcgcgcgcg atatctgctt tatgcgcata atggcttttc
     9181 agcctgatgt gaaagggatc gccactgtct tgattttgac ggtggatata tttagcgcgt
     9241 ccatgattgc gcagaatttt accctgcgtc tgtaattcat tgagatcgcg acgaatggtt
     9301 tctttactga cctttagctg ttcagagaga gcttccgtgg tcaggctggt atggttcagc
     9361 agcaggtcga ctatcgcttg ctggcgtgcc gctttcatat ttttcacccc atcattcgat
     9421 aaccgcccgc gtcatgcggg cgtaccggta tgagattacg gtgttacccc tttttttaaa
     9481 agaatattcc cgtacttcgc tcgttcgcct gtgataacga tcgcaaaggc tttttgcgcc
     9541 cgttcataaa acgcaaaacg attgatgcgg atgatgtcag gacacggggc ttgtagtgaa
     9601 agcgcattac ggtaacgtcg ttctacttca ggatcgagag tgtcaccttc taccgccgcc
     9661 atcatcacca gcggcggtgc ataactgtcc agttcaaata acgggataat cgcctggagc
     9721 aagtcgctca ccaacaggcc atcagcgcgg atcacctgcg gtcccatcga atgggcggga
     9781 aagtgagcat cggaaaaaat aatttcatct ccatgtccca tctctgccag cactttcaat
     9841 agttcgggag aaattaacgg cgaaattgtt ttcagcattt cacacttcct ctataaattc
     9901 aggttcagtt tgcgggtaga aataacggta ctgataatga atctgtgcgc gggcttcttc
     9961 agggctgtta aattccccta cgccatacca accgaacagc gcagctcctg cgacggtcgt
    10021 ttcggcgtcg tcgagaactt ttaccggaat atcaagcata ttggctttaa tctgattcca
    10081 caatgtgttg cgacttcctc caccgactaa caataattca gaggccttaa agtgcccgat
    10141 tttttccagc atctgtagat tgcgctgtaa ttgcgcagtt aacccttcca gcgccgcgcg
    10201 atagaaatgc ccccgcgtgg tattaagcgt cactccttgc cagccagcgt tctgacacga
    10261 caataaatca cactgcattt ttacgccatc cgcgctaggc gcgataagac gagcttcttc
    10321 aatcaacatt tgccagggtg tttcagccgt ccagaacagt tttctcaccc attccagcac
    10381 gccggatgcc agccattgca tacctgggtt atacaaccct gcctggctat ccagttcgca
    10441 ggtggaaccg gcgtactgac ttaacagcga agtatcaacc tgggcgctac ggactattaa
    10501 aatttcccat gtaccggaag agagcacggg ttcattttgg tcggcaccag cgccaaaaag
    10561 ggcgaactgg gtatcgtgac ctgcggaaat caccggtatg ccaacgggta agccgagcat
    10621 tgctgcggcg ctgttctgta gcgtaccaat ctgttcaccc gcttccacca gacgagggaa
    10681 gagtcggcgt ggaataccag tggcttgtaa aatttgcgga ctgaaatcgc gttgctggat
    10741 atccagcatc tggctggttc cggccatcgt gatatcagta gtgaattcgc cggttaaacg
    10801 gtggttaatc agcgacgaaa taaagaacca ggcgtgcgtg cgttccagca gttgtggatg
    10861 attttctttc aaccacacca acttatataa cgtattgaaa ctaaaggctc cgacgccaga
    10921 aatagcctgc aaccgctgtg cggagatcaa ctgttcaata ttgtccataa ccgctgctgt
    10981 tcgcggacat ttccagctaa tgatcggata gagcagattg ccttgcttat ctaccagagc
    11041 gccatccaca ccaaaggtgg tgacggcgat accgcggatg tggcattcag tcagttcact
    11101 attgatttgc cgacagcaat cagcaaagcg ttgcaaaatg gcgtctaaag accactggtg
    11161 ccaggtgttg ttttccatcg cgatatcgct ggcattaggc gttgaggcgc gggcaacaat
    11221 ttttccctgc cgattaaccg cgatggccct gacattggtc gcgccacagt cgagtaccag
    11281 gataacttct tgtttcataa tcgctcctgc aatacttcaa tatccatgcc gtgcgcagcc
    11341 caggcagaag gacgatacac tttggtctct tcaacgttgt gcatacatac cgggatacgc
    11401 agcatggagg cgagagtgat aaagtcagcg ccaacgtggc cgatggtcag aaccccatgg
    11461 ttagcgcccc agttcgccat taccgagtac acatccgtaa acggcccttt accggtgagg
    11521 cgcggtgcaa accaggtggt tggccaggtt gagttggtgc gtttgttgag gatgtcatgc
    11581 acatccttcg gcaattccac gctccagcct tccgcgattt gcagtaccgg tcccaggcct
    11641 ttgatgatgt tgacacgagt catggtgaac gggacgccgc cttcggtaag gaagcgggaa
    11701 gagtaaccgc cgccacggaa gtattcgtgg atcgccgggc accattcggt agcggcgagg
    11761 caagcgtcag cctcttgctg agagatttcc cagtgcggct tcatcgtcgg gttaccttcg
    11821 ctgtcgcgtt gtttacagga accgtccagc gcagcagaac ctgagttgat caaatggatg
    11881 atgccgtgtt ctgccagtcc atcgagttta tgccccgtta cacgctcaat tgcttctggt
    11941 gaccagtagg tacgcacatc ggcaaatacc tgagcggtgc cggtgagctg gtgacccatt
    12001 agcattgcca cgccgttaag actgtcgttt tcggtcgcca cgacaaaggg ttcgcgcacg
    12061 ccattccagt caaatgaact gttgaggatc gcttcggcgg tgtcaccatt gggatattga
    12121 tcggtccagt gacgttgccc ctggaagccc gcagcgatgg cgttgtagcc aagtgattct
    12181 tccacgcgac caatatcggc cagtttgctg ttgccttgca tcatgtcgcg gatacacatc
    12241 gccatcagta aactttcgcg cagaactgcg cggctttgct cggcattgcg ttgatactgt
    12301 ttgttatttt catcttcgcc atagcggaag tttttatcag cccaggccag tgccatttcc
    12361 agttcggctt cgtcgtaaat cttctgatcg atacggcgac gcagttcggt catatccacc
    12421 gcctggactt tcattcccag ccaggattca aagaagttgt gatcaacgat ggaaccggcg
    12481 atacccatcg aaacgccgcc cagcgacaga tagcttttac ctttcatgct ggcgacggcc
    12541 aaaccggcgc gggcaaagcg cagcagtttt tcttcaacat cggcaggaat cgatgtgtca
    12601 tcggcatcct gaacgtcatg accgtaaatg gagaatgccg ggatgccttt ctggctgtga
    12661 gctgccagag ccgctgccag gtaaacagcg ccgggacgtt cagtgccgtt aaagccccaa
    12721 atggctttcg ggcgggttgg atccatgtcg atggtttcac tgccatagca ccagcaaggc
    12781 gttaccgtaa tggtgaggcc tacattctgg ctgctgaatt tttcttcgca agcagcggct
    12841 tcagccatac ccgcgataca ggtatcggaa atgacacact cgacggcagc tccgcaggca
    12901 tggcgcagtt tctcggtcag cagtgcggcc gtagctttcg ccatattcat tgtttgttct
    12961 tcaagcgact cacgaacacc catgcgacga ccgtcaataa ccgggcggat accaattttc
    13021 ggtaagctga tttttttcat tacagattcc tcactttatt cggaaaatat gttcagttag
    13081 ttgccgtttg agagcggaaa cgggcaaaga taaagatgac cgcgaagcag agtgcgggga
    13141 tcagttcagc agtggggatg ttgcccgccg cgtcactgac aaaacccatg accggagtga
    13201 caataccgcc gccaataatg gtcataacga tgaaggacga accgtatttg gtgtcctggc
    13261 cgagattctt aatgcccagc gagaagattg ttgggtactg aatcgacata aaggcgctgc
    13321 ataaagtcag ggctattaag cccacatgac cgccagcgaa ggctgagatc aggcacagtg
    13381 ccatagcgat taatgcgtag gcggccagga ctttgtgtgg tgcgaagcga ctgatgagcc
    13441 aggtaccggt gaaacgacca ataaagaagc acaccatggt tccggttaaa tagttagcgg
    13501 caaagcctgc agtcatacct ggaatttctt ctacagcgta gcgaatcaaa tagctccagc
    13561 aggccgtttg tgcgccaacg tagcagaatt gcgctaatac tgcccagcgc cagtggcgaa
    13621 tacgcgccag gcgagaaagc gatgcggaga acgatccttg tttggcgtca ctgtgattat
    13681 cactctgcaa tgccgggaat ttcgtcagca tgatcagcag ggcgaccagt aacacgatag
    13741 ccacgatgat catataaggt gtctgtaccg ataataccag gctgtgttta tacgcactca
    13801 attgctctgg agacatttta tcgagaacgt cttgcgattg atgtggcacg ttagacaaaa
    13861 taagactttg cccaaagaca accgcgataa ttgcgccaaa cgagttaaat gtttgcgcaa
    13921 gatttaagcg gaagtgacca ctgctttccg gccctaatac cgtaacaaaa gggtttgcgg
    13981 cagtttccag acaacctaat ccggctgcaa taataaatag gccaactaaa aataaggtgt
    14041 agttcattat ttctgcagcg ggccagaata atgcagcgcc cagggcatat aaaaataacc
    14101 cagtaataa
//
LOCUS       DAJTMC010000102        13938 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.102, whole genome shotgun sequence.
ACCESSION   DAJTMC010000102 DAJTMC010000000
VERSION     DAJTMC010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13938
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.102"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(<1..206)
                     /locus_tag="OO130_002741"
     CDS             complement(<1..206)
                     /locus_tag="OO130_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HCR5366083.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECA"
     gene            complement(594..1340)
                     /gene="cutC"
                     /locus_tag="OO130_002742"
     CDS             complement(594..1340)
                     /gene="cutC"
                     /locus_tag="OO130_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HCR5366084.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQWVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLNTLSNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DAHADEYSRYVVDGAAVAEMKGIIERHQAK"
     gene            complement(1354..1920)
                     /locus_tag="OO130_002743"
     CDS             complement(1354..1920)
                     /locus_tag="OO130_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR5366085.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNVTAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEYERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            2136..3869
                     /gene="argS"
                     /locus_tag="OO130_002744"
     CDS             2136..3869
                     /gene="argS"
                     /locus_tag="OO130_002744"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HCR5366086.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVSTPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            4046..4534
                     /locus_tag="OO130_002745"
     CDS             4046..4534
                     /locus_tag="OO130_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HCR5366087.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDISAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGVKKPDIEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
     gene            complement(4654..5046)
                     /gene="flhe"
                     /locus_tag="OO130_002746"
     CDS             complement(4654..5046)
                     /gene="flhe"
                     /locus_tag="OO130_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HCR5366088.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     TRQPASGLMTLVAWRYQLIGPTPSGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEVPGGGRLIPPLKVQRNEVIVNYR"
     gene            complement(5046..7124)
                     /gene="flhA"
                     /locus_tag="OO130_002747"
     CDS             complement(5046..7124)
                     /gene="flhA"
                     /locus_tag="OO130_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HCR5366089.1"
                     /translation="MSNLAAMLRLPANLKSTQWQILAGPILILLILSMMVLPLPAFIL
                     DLLFTFNIALSIMVLLVAMFTQRTLEFAAFPTILLFTTLLRLALNVASTRIILMEGHT
                     GAAAAGKVVEAFGHFLVGGNFAIGIVVFVILVIINFMVITKGAGRIAEVGARFVLDGM
                     PGKQMAIDADLNAGLIGEDEAKKRRSEVTQEADFYGSMDGASKFVRGDAIAGILIMVI
                     NVVGGLLVGVLQHGMSMGHAAESYTLLTIGDGLVAQIPALVISTAAGVIVTRVSTDQD
                     VGEQMVNQLFSNPSVMLLSAAVLGLLGLVPGMPNLVFLLFTAGLLGLAWWIRGCEQKA
                     PAEPKPVKMAENNTVVEATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRIRSIRK
                     KFAQEMGFLPPVVHIRDNMDLQPARYRILMKGVEIGSGDAYPGRWLAINPGTAAGTLP
                     GEATVDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGRQE
                     AQQLLDRVAQEMPKLTEDLVPGVVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAP
                     IQSDPHELTAVVRVALGRAITQQWFPGKDEVHVIGLDTPLERLLLQALQGGGGLEPGL
                     ADRLLAQTQEALSRQEMLGAPPVLLVNHALRPLLSRFLRRSLPQLVVLSNLELSDNRH
                     IRMTATIGGK"
     gene            complement(7117..8220)
                     /gene="flhB"
                     /locus_tag="OO130_002748"
                     /pseudo
     CDS             complement(7117..8220)
                     /gene="flhB"
                     /locus_tag="OO130_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
     gene            complement(8303..8947)
                     /gene="cheZ"
                     /locus_tag="OO130_002749"
     CDS             complement(8303..8947)
                     /gene="cheZ"
                     /locus_tag="OO130_002749"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HCR5366090.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLAEARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(8958..9347)
                     /gene="cheY"
                     /locus_tag="OO130_002750"
     CDS             complement(8958..9347)
                     /gene="cheY"
                     /locus_tag="OO130_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HCR5366091.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFDKLDM"
     gene            complement(9362..10300)
                     /locus_tag="OO130_002751"
     CDS             complement(9362..10300)
                     /locus_tag="OO130_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HCR5366092.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPVGMLAMRQAGAWTLAQNEASCVVFG
                     MPREAINMGGVCEVVDLSQVSQQMLAKISAGQAIRI"
     gene            complement(10303..11115)
                     /gene="cheR"
                     /locus_tag="OO130_002752"
     CDS             complement(10303..11115)
                     /gene="cheR"
                     /locus_tag="OO130_002752"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005063150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HCR5366093.1"
                     /translation="MRLPGCSRYSFRRISQLIYQRAGIVLADHKRDMVYNRLVRRLRS
                     LGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHHFPLLADHARRRSGEYR
                     VWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVLEKARSGIYRHEELKNL
                     TPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQYTVPGPFDAIFCRNVMI
                     YFDQNTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLRGQTVYALSKD"
     gene            complement(11253..11819)
                     /gene="flhC"
                     /locus_tag="OO130_002753"
     CDS             complement(11253..11819)
                     /gene="flhC"
                     /locus_tag="OO130_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HCR5366094.1"
                     /translation="MSEKSIVQEARDIELITLGARLQMLESETQLSRGRLIKLYKELR
                     GSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLYLEQC
                     PQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLCQPPS
                     RAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(11822..12172)
                     /gene="flhD"
                     /locus_tag="OO130_002754"
     CDS             complement(11822..12172)
                     /gene="flhD"
                     /locus_tag="OO130_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HCR5366095.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVNDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
     gene            12950..13378
                     /gene="uspC"
                     /locus_tag="OO130_002755"
     CDS             12950..13378
                     /gene="uspC"
                     /locus_tag="OO130_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HCR5366096.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCHKHH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
     gene            complement(13385..>13938)
                     /locus_tag="OO130_002756"
     CDS             complement(13385..>13938)
                     /locus_tag="OO130_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HCR5366097.1"
                     /translation="LHTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLYY
                     LNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAAN
                     ELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISDL
                     KQIVPRSAESQQRDKVATFPKLA"
BASE COUNT         3588 a         3695 c         3268 g         3387 t
ORIGIN      
        1 gcacattccg cacgtatccc tttctggttc gaaaagtgga cagttccctg atattcctca
       61 aaaggtttac tcatcgagtg gcaggaaagg caaaatgctg tatccgacgt tttatgtaag
      121 gctttttgcg ccagcaataa cccgccgcca ccaaccacaa ccgctatcag caaaaacaat
      181 aacccaatgc gttttttccc tcgcatagat tcctctaaat aaattccaga acaccgaaat
      241 cattggataa ttaaatacac aacgaattaa ccgcttgcat aactcggcac aacactgcct
      301 gaaataatcg ataaagaata tgatttatta caatgtaatc attaattgct aaggaataac
      361 cctgttgtta tttagaatat atatttgtca gttttagcaa tttaaacccc ataattaatg
      421 tgaatatata aaatattttt agcacaacgc tatgttcact gtcacccgct caacagatca
      481 cggttatcgt acgtttttta tactgctcag ggataaaaaa aggcccctgt cgaaattgca
      541 ggggcctggt acgagcaagc atcatattgg gcgacatgat gcaacggtaa aaatcatttg
      601 gcctgatggc gttcaatgat tcctttcatt tcagcaaccg ccgccccgtc tacgacataa
      661 cgcgaatact cgtccgcgtg tgcatctgat gacatggaca atccttgatt acgataacgc
      721 atcggtgaag cttgccacgc tcccgcggag ctatggactt ccagcactcc ggcatcgagg
      781 aagtggtgca ggttttctgc acggactcct gctccggcca taatgattgg agcatcacga
      841 tgggcaataa gttccataat ttttgataaa ccttgcagtg catctgattt ttgccctgat
      901 gtcagcaccc gggcgatgcc caattccgca agattactga gtgtatttaa agggttagcg
      961 cacatatcga aggcgcgatg aaaagtcact gccagcggac cggcagcagc cattattttt
     1021 tccattcgtg gcatatcgac attcccgtca acatcgagca cgcccgtcac cagtccagga
     1081 aaacctaatt cgcggactgt gcgcacatcc tcaagaatgg cggcaaactc accgtcgctg
     1141 tagcaaaaat caccaccgcg tgggcggata atcggatgca cagggatcgt cacccactgg
     1201 cgcacggatt tcagtacacc caacgacggc gttaagcccc cctcttttgg ggctgcgcat
     1261 aattcaactc tgtctgcgcc gttttgctgc gccgttagtg cacattccat gctgtaacag
     1321 caaatttcca gtaatgccat ttttactcct taattacgcc gactgctcgc tggcaacgat
     1381 ctcttcaatg gaccacggat gaaacttaat agttgttttg ccatcagtga ccgccagcgt
     1441 cggattcggt aatcgctcat actcaccttt tggcgaactg gttttcactg agatccccgg
     1501 taggctgagt ccttcatcag aaagcaatgc cagcgcacgg gcattcagcg tttctggatc
     1561 acccggcaga acgatttcaa tatgttccca accttcgtgt gggtaacgtt tttccccggg
     1621 ccacggtagc tccacaatgg aaaactgcca gtgcgcaacc tgtaccggtt catgcaattt
     1681 aaacagacaa atcggtctgc cattgatcat attttctgac aaaagctcgc cacactgttc
     1741 aaaaccgcga cgccagcgct cagcggtgac gttttgatgg caacgcaaag aaatgtgatc
     1801 ggcagtcagc ggagtgatat ccaaccccag acggcgggag agttcatcta atgcgtgggt
     1861 aaatcgcggt aaatccgatg caatatcctg cagctcgtcg atagattgcc agttcgccat
     1921 aatcactctt cgtctttcag taaaagcgtt aatttaccct gttgccctgc gccaaccaac
     1981 cgctgatttc acgccgcttc tgatgcaata gtgaaaacgg caatacgcca cgcgcacgtt
     2041 gctgacgaaa acagccattt gcagtatact cccgccctaa tttctttaac tggtgcgggc
     2101 aatttttgct cgcttcatca atgtaaggta ttccggtgaa tattcaggct cttctctcag
     2161 aaaaagtccg tcaggccatg attgcggcag gcgcgcctgc ggattgcgaa ccgcaggttc
     2221 gtcagtcagc aaaagttcag ttcggcgact atcaggctaa cggcatgatg gcagttgcta
     2281 aaaaactggg tatggcaccg cgacaattag cagagcaggt gctgactcat ctggatctta
     2341 acggtatcgc cagcaaagtt gagatcgccg gtccaggctt tatcaacatt ttccttgatc
     2401 cggcattcct ggctgaacat gttcagcagg cgctggcgtc cgatcgtctc ggtgtttcta
     2461 cgccagaaaa acagaccatt gtggttgact actctgcgcc aaacgtggcg aaagagatgc
     2521 atgtcggtca cctgcgctct accattattg gtgacgcagc agtgcgtact ctggagttcc
     2581 tcggtcacaa agtgattcgc gcaaaccacg tcggcgactg gggcactcag ttcggtatgc
     2641 tgattgcatg gctggaaaag cagcagcagg aaaacgccgg tgaaatggag ctggctgacc
     2701 ttgaaggttt ctaccgcgat gcgaaaaagc attacgacga agatgaagag tttgccgagc
     2761 gtgcacgtaa ctacgtggta aaactgcaaa gcggtgacga atatttccgc gagatgtggc
     2821 gcaaactggt cgacatcacc atgacgcaga accagatcac ctacgatcgt ctcaacgtga
     2881 cgctgacccg tgatgacgtg atgggcgaaa gcctctacaa cccaatgctg ccaggaattg
     2941 tggcggatct caaagccaaa ggtctggcag tagaaagcga aggggcgacc gtcgtattcc
     3001 ttgatgagtt taaaaacaag gaaggcgaac cgatgggcgt gatcattcag aagaaagatg
     3061 gcggctatct ctacaccacc actgatatcg cctgtgcgaa atatcgttat gaaacactgc
     3121 atgccgatcg cgtgctgtat tacatcgact cccgtcagca tcaacacctg atgcaggcat
     3181 gggcgatcgt ccgtaaagca ggctatgtac cggaatccgt accgctggaa caccacatgt
     3241 tcggcatgat gctgggtaaa gacggcaaac cgttcaaaac ccgcgcgggt ggtacagtga
     3301 aactggccga tctgctggat gaagccttgg aacgtgcacg ccgtctggtg gcagaaaaga
     3361 acccggatat gccagctgac gagctggaaa aactggctaa cgcggttggt attggtgcgg
     3421 tgaaatatgc ggatctctcc aaaaaccgca ccacggacta catcttcgac tgggacaaca
     3481 tgctggcgtt tgagggtaat accgcgccat acatgcagta tgcttacacg cgtgtattgt
     3541 ccgtgttccg taaggcagaa attaacgaag agcaactggc tgcagctccg gtaatcatcc
     3601 gtgaagatcg tgaagcgcaa ctggcagctc gcctgctgca gtttgaagaa accctcaccg
     3661 tggttgcccg tgaaggcacg ccgcatgtga tgtgtgctta cctgtacgat ctggctggtc
     3721 tgttctctgg cttctacgag cactgcccga tcctcagcgc agaaaacgaa gaagtgcgta
     3781 acagccgtct gaaactggca caactgacag cgaagacgct aaagctgggt ctggatacgc
     3841 tgggtattga gacagtagag cgtatgtaat cgatttttcg tgagagtaaa gcctgatcag
     3901 ggttacctga tcaggctttt ttattgccat ctaaatgtat tctgaaaatg gacatgccat
     3961 tgttttctga ctgttggata agaggccaga agcgtaatat ccggccccag ggaaacgata
     4021 acggttgaat ttaaggaata ccgcagtgtt taaatttctt gtattaacat taggcattat
     4081 ctcttgccag gcttacgcag aagatacggt tatagtaaac gaccatgaca tttcagccat
     4141 caaagattgt tggcaaaaaa attcagatga tgatactgac attaacgtga tcaaatcatg
     4201 cctgcgacaa gaatacaatc tcgtcgatgc gcaattaaat aaagcctatg gtgaagctta
     4261 tcgttatata gaacaagtgc cacgcacagg tgtaaaaaaa cctgatatcg aacaacttaa
     4321 cttgcttaaa aaatcacagc gagcctggct ggattttagg gacaaagaat gtgaattaat
     4381 cctttcaaat gaggacgttc aggatttaag tgacccttat tctgaatcag aatggctctc
     4441 atgtatgatc atacagacca atacgcgaac tcgccagttg cagctatacc gtaactctga
     4501 agatttttat ccaagccctt tgacaagagg ataattcaca tctttttggc atgttttgtt
     4561 gcaagctatt cctgataaat aattgcaaca agacatcgag cctttttcac tgagttatta
     4621 aacatactcg cgagcgcgta atttttttgt ccttcagcga taattcacaa tcacttcatt
     4681 acgttgtacc tttagcggtg gaattaaccg cccaccgcct ggcacttccc agataaatcg
     4741 caacggttct gctgccgcta taccggaaaa ggccacggtg gttccgctct gcccctctaa
     4801 ttcgacacaa cgagattgcg aacacaagcg aacccgcagt cctgaaggtg tcgggccgat
     4861 aagctgataa cgccacgcta ccagcgtcat caatcctgaa gcaggttgtc gcgtagaaag
     4921 aggcgcagac gacatcgact caccgcgatg atttagcgta attcctacac tacttgcctg
     4981 ccacatcccc tccccggcgg cttgcaccag cagcggaaac aataatattg ctaataaggt
     5041 tctcattatt tgccgccaat tgtcgccgtc atgcggatat gtcggttatc agacagttcc
     5101 agattcgaca ggaccactaa ctgcggcaag ctgcggcgca ggaagcgaga caataatggt
     5161 cgcagcgcgt ggttcaccaa caatactggc ggcgcaccca gcatctcctg acgggatagc
     5221 gcttcctgag tttgcgccag taaacgatcc gccagccctg gctccagtcc tcccccgccc
     5281 tgcagcgcct gtagtaacaa acgttccagc ggtgtatcga ggccaataac atggacttca
     5341 tctttgccag gaaaccactg ctgggtaatc gcccgtccca acgccacgcg cacgacggcg
     5401 gttaattcat gtggatcgct ttggatgggc gcatgttccg ccagcgtttc gagaatggtg
     5461 cgcatatcgc gaatcggtac tttttcatcg aggagatttt gcagcacttt atgcagtgtg
     5521 gtgagcgtga cgacgccagg aacgagatct tccgtcagct ttggcatctc ctgggcgacg
     5581 cgatccaaca gctgttgcgc ctcctgacga ccaaacagct ctgcggcatg ctggctaatg
     5641 aggtggttaa gatgcgttgc taccaccgtg ctggcctcaa ccactgtgta cccctgaatc
     5701 tgcgcctgtt cttttagcgc actttcaatc cagatagcat tcaggccaaa tgccggatcg
     5761 acggtcgcct caccaggtaa cgtcccggca gcggttccag ggttaatcgc caaccagcgc
     5821 cccggataag catcaccact gccaatctcc acgcctttca tcaaaatgcg atagcgggca
     5881 ggttgcagat ccatattgtc gcgaatgtgc accactggcg gcagaaatcc catctcctgg
     5941 gcaaatttct tgcggatact gcgtatacgg cccaacaact caccatcctg ctggaaatcg
     6001 accatcggga tcagtcgata acccacttcc attcccagag aatcttccag ttgtacatcg
     6061 ttccacgtcg cttcgacaac ggtattattc tctgccattt ttaccggttt gggttcggca
     6121 ggcgcttttt gttcgcatcc gcgtatccac caggccagcc ccagcaatcc ggcagtgaac
     6181 agcaaaaata ccaggttcgg cattccaggc accaagccga gtaaaccgag cacggcggcg
     6241 cttaacaaca taacgcttgg gttactgaaa agctgattca ccatctgctc gccaacatcc
     6301 tgatcggtgc tgacacgcgt aacgatgacc cccgcggcgg tagaaatcac cagcgccgga
     6361 atttgtgcca ccagaccgtc gccaatggtc aatagcgtat aactttccgc cgcgtgtccc
     6421 atgctcatgc catgttgcag cacgccgacc agcaacccgc cgacaacgtt aatgaccatg
     6481 atgaggatcc cggcgatggc atcgccgcga acaaacttac ttgccccgtc cattgagccg
     6541 taaaaatcgg cttcctgagt cacttcggag cggcgttttt tcgcctcatc ttcaccaatc
     6601 aatccagcgt taaggtcggc gtcaatcgcc atctgcttac ccggcatacc atcgagaaca
     6661 aagcgcgcac ccacttctgc gatacgcccg gcacctttgg taatgaccat aaagttgatg
     6721 atcacgagaa tgacaaacac cacgatacct atagcgaaat tgccaccaac gaggaagtga
     6781 ccgaacgctt cgaccacctt ccctgccgcc gccgcgccgg tatgcccttc catcaaaatg
     6841 atacgggttg aagccacgtt aagtgccaga cgcaacaacg tagtaaacaa cagaatggtc
     6901 ggaaacgcag caaactcaag cgtgcgctgg gtaaacatcg ccaccagcaa caccatgatc
     6961 gacaaggcaa tattgaaggt aaacaacagg tcgagtatga atgcgggcag tggcagcacc
     7021 atcatcgaca agatcaacag gatcaaaatc ggtccggcaa ggatctgcca ttgtgtcgat
     7081 ttcaggtttg cgggcaggcg cagcatcgcg gccagattac tcatgggtcg gtttctcgtt
     7141 aataaaatcc agggcttccg gcaccggaag atgagtaggt tgtacagggc gctgtccacc
     7201 agccagacgc cagcgtttca gttgccagat ccaggccagc acttccgcca ccgcggcgta
     7261 cagttgaccc gggatttgtt gaccaatctc cgcatgtcga tacagcgctc gcgccagcgg
     7321 cggcgcttca agcgtcggga cgttattttc agcgccaatt tcacgaatgc gcagcgcgac
     7381 cagccctgca cctttagcga ccactttcgg tgcgctcatt ttgttttcgt catactgcaa
     7441 cgctaccgaa tagtgggtcg ggttattgac aatgacatcc gctttcggca catcggccat
     7501 catccgacgc cgtgcggcag ctcgctgcat ctgacggatc cgtcctttaa catgggggtc
     7561 gccttcgctt tgtttgaact catcacgaat atcctgccgc gacattcgca gctttttcag
     7621 gtggctgaag atttggaaaa agacgtcaaa tcccaccatt ggaatgacac caagcaccac
     7681 cagcagtgcg catagcccta ccaaatccat cgcattaccc atggcggtaa tcggagactc
     7741 ggccagtaag cgcatcatct gcggccagtg atgccagaga aaaaaccccg tcacgctgcc
     7801 aaccaagatg gttttcaaaa ttgctttaag caactccgcg ccagtctgtg ccgagaacat
     7861 ccgtttaatg cccggtagcg ggttgagttt ggaaaacttc ggctgcaagg attttccgct
     7921 aaataccagc ccccccagca tgaccggaga aatgagcgct accagcacca cgccgctaat
     7981 cagcggtagc agcgccagca tggcttctct gatcagcaga ataatctgcc cgaggatcag
     8041 attcgggtct ttgataatac tgtgatcaaa atgcagccca gcggagagca tgcccgacaa
     8101 tcgacgggcc agcgacacac cgccgaacca gataacacac acgcccacca gcaaaatcag
     8161 cagtgaggtc agttcacggg aacgcgggat ttagggcttt tgctgccaca catcaagcat
     8221 agtgtgcgtt tgtcggatgc ggcgtcatcg ccttatcaga ccgcctgata tgacgtggtc
     8281 acgccacatc aggcaataca aatcaaaatc caagactatc caacaaatcg tccacctgat
     8341 cctgactggc taccacaccg gctttgctgg tatcgacctg aggtccatta agcaaactct
     8401 ggttttcacg ttttggacgc gactcctgtt ccgggatgtt ttccaacagc accatcagca
     8461 actggcgttc gatctcctga atgacatcca tcatccgctt aataacctgc ccggtgagat
     8521 cctgaaaatc ctgcgccatc atgatttcca gcagttgcgc gttagtaaag ctggtatgcg
     8581 cgggtacatc tgccagaaat tgtcgtgtat ctgttaccag ttcacgggcc tcggcaaggt
     8641 caatcggatc ggcaaaccag tcatcccaac gttgggttaa cgcttttgct gatttctcca
     8701 tttgatcctg atgcggttgt gacgcctcaa cactgttcag cgcccgctcc gcagcctggg
     8761 cggtcatctg cacaacatag tacaaacgat cgcgcgcatc ggggatggct tccgccgctt
     8821 cggcaatggc ctgatccagc cccagttccc gcaaactgtc gcgcagcata cgcgtcaggc
     8881 tgccgatgcg cgcaatgata tcgccagctg aatgctcgtc agcaggtttg attgatggtt
     8941 gcatcatagt cgcatcctca catgtccagt ttatcaaaga ttttgttgag tttttcctcc
     9001 agcgtcgcgg cggtaaatgg cttcaccaca tagccactgg cccccgcttg cgccgcagca
     9061 atgatgttct ctttcttcgc ttctgcagtc accattaaca ctggcaatgc cgacatcgcg
     9121 ccatccgcac gaattgtttt cagcaattcc aggccatcca tattgggcat gttccagtcg
     9181 gagataacaa atccataacc gcctgcctgc aacttattga gagcgtcgac gccatcttcc
     9241 gcttcctcaa cattattgaa tcccagctct ttcagcaggt tacgcactat gcgtcgcatg
     9301 gtggaaaagt catccacaac caaaaattta agttctttat ccgccatttc acactcctga
     9361 tttaaatacg tatcgcctgt ccggcactaa tttttgccaa catttgctgg cttacctggc
     9421 taagatcgac cacttcgcag acaccaccca tattgatggc ctcgcgcggc atgccgaaca
     9481 ccacgcaact tgcttcgttt tgcgcaaggg tccatgcccc cgcctgacgc atcgccaaca
     9541 ttcccaccgg gccgtcgtga attttgattt gataatttgc gccactacgc gccagttcca
     9601 tatgccgatc gcccggcgca atataggcat gccccggcaa gacacgttct ccgtcttcgg
     9661 cttctttaac cccgatctgg caaagcttat taagtctgtc ggcaaaagag cgggtgaaac
     9721 cgggcggcat atgttgggta attaacaatg ccgggctgga aagcggcaac ggttgcagta
     9781 cgtgacgaat tgcctcagtt ccacccgttg aagcaccaat cgcaatcagt ttttcagaac
     9841 tcaacaacgg acccgccttc agcgttgtcg gtgctgacaa tggcttatgt gctgcaaggc
     9901 tcgcctttgc tgccgtacgc accttttcag caatcatttc gctatacgcc agcattcctt
     9961 cgcgaatacc cagttgcggt ttggtgacaa aatctattgc ccccagctcc agcgcgcgca
    10021 gcgtgacttc tgatcctttg ccggtcaagg aagaaaccat cacaacgggc attggacgca
    10081 aacgcattaa tttttcgagg aaatccagtc cgtccatccg cggcatttca acatccagcg
    10141 tcagcacatc gggattgaat ttcttaatca agtcacgcgc gaccagcgga tcaggcgcgg
    10201 tcgccaccat ttccatgtcg ctatggctgt tgatgatttc tgtcatgatc tggcgcatca
    10261 gtgccgaatc atcgacagat aacaccctga ttttgctcat cgttaatcct tacttagcgc
    10321 atacaccgtc tgaccacgca gcgtgaagcg gcgctcaagg tggctaaagt tttcagagtg
    10381 acccgcaaac agcaatccgt cgggtttaag gagcggaaca aagcggcgca aaatctcctg
    10441 ctgggtattt tgatcgaagt agatcatgac gttacgacag aagatcgcat caaacggccc
    10501 cggcacggtg tactgtttcg ccagtagatt cagcggggca aaatcaacat agttcgccag
    10561 ctcctgacgc acgcgtacca gcccttcatg cggtcccgtc cctcgcatga aataccgttg
    10621 cagttgctgc ggcgtcaggt ttttcaactc ttcatggcga taaataccgc ttctggcttt
    10681 ttccagcact tcggtgtcga tatcactggc aaacactttc cagcgtccgg gcgcggtgcc
    10741 caatgtgtca gccagcgtca tcgcaatgct gtacggctct tcgccggttg aagccgccgc
    10801 gctccatacg cgatactcgc cagaacggcg acgtgcgtga tccgcgagca gagggaaatg
    10861 atgtgcctca cggaaaaatg ccgtcagatt cgtggtcagc gaattgataa acgcctgcca
    10921 ctcaccgctg tgctgattag attccagcaa gttcagataa tgaccgaaat ccgtcagtcc
    10981 cagcgaacgc aaacgacgaa ccagtcggtt gtaaaccatg tcgcgtttat gatcggccag
    11041 gacgatcccg gctcgttgat agatcaattg acttatccgc cgaaaactgt accgagaaca
    11101 accaggtaac ctaataagat aagcacgaca tcatccttcc actgttgacc atgacaggat
    11161 gttcagtcgt caggcgttaa cgcgcgattg gggcaaaaaa aagcagcggt acgtcgttac
    11221 cgctgctggc atgttgcgcc acaccgtatc agttaaacag cctgtactct ctgttcatcc
    11281 agcagttgtg ggataatatc ggcaggattc tgggaaagtt tacgtctttt tactgcccgg
    11341 gatggcggtt gacataagct gcaggcaaag ctgccaacag gctggtgagc gtgggtaata
    11401 aaattgccgc cgcagcagtt gcagctggaa agttgcagta atccactttc aacaaaccgc
    11461 accaatgtcc aggcacgggt taatgccagc agtggtcctt cttctgcttg tgggcactgt
    11521 tcaaggtata aacggtaggc tttgatcacc gcatcgacgc cattacacaa acctgttttc
    11581 agtaaaaact gccatgcatt acagaacatc gaagcatgaa cgttttgttc ccaggtcata
    11641 aaccagtcgg ttgagaatgg cagcatgcct ttcggcggtg ggcttccgcg cagttcttta
    11701 taaagtttta tcaggcgtcc gcgacttaac tgtgtttcgc tttccagcat ctgcaaacga
    11761 gcgcccaggg tgatcaattc aatatcccgc gcttcctgaa caatgctttt ttcactcatg
    11821 atcaggccct tttcttgcgc agcgcttctt caggctgatt aacatcattc agcaagcgtg
    11881 ttgagagcat gatgccggta tgaatttgct ggagatcgtt aacgcgggaa tcttgcgtca
    11941 actgagtaat cgtctggtgg ctgtcaaaac ggaagtgaca aaccagttga ttggtttctg
    12001 ccagcttaac catttgcgga agagtcagtg ccgctaacgt tgtcgccatt tcttcattta
    12061 tgccgagacg aaacatagcg gacgctttgt cctgaacaat caaacgctgt gcaagtagta
    12121 aatatgacaa gttgatgtca taaatgtgtt tcagcaactc ggaggtatgc attattccca
    12181 cccagaataa ccaactttat ttttatgcgg tttcaccgca ccccgtgatg tcgccgggaa
    12241 gccccggtaa aaaataatta gcattagaat agtgcgataa gctgcaataa gcagaaccac
    12301 ctttttggtt taatatgtcc ttacaaatag aaatgggtct ttacacttat ctaagatttt
    12361 tcctaaatcg acgcaactgt actcgtcact acacgcacat acaacggagg gggctgcgat
    12421 tttcaataat gtgtgatgca gatcacacaa aacactcaat tacttaacat aaatgtataa
    12481 gccatacttt tgttttgggt gtatttcaat ctgttaaaaa gtttttcgct acgctagcaa
    12541 gcaaaaatga aactggaata atcgaaatgg gatgttgcgc acagttaaaa taactcaccg
    12601 taaataatca tctgctataa ataatcactt tcatgcaata ccagataagc tatttttaaa
    12661 cagacactta ccgcacaaca aactaattaa atacaaagaa aggcaacaat caacttaatg
    12721 ttaatgaaat gttttacata tattaaacac ttgtttaatg cggagaatca gtacactatt
    12781 ttcttataat tacatttgaa attatatgat cagcgggagt gattgattaa ttgtctttac
    12841 taataatcag actaatattt acctgtttga ccgagttggg attgcgtcgt ttctccatta
    12901 ggagtaaagc tttaatgtca cctgaagttc acagaataag gaacaggtta tgagctatag
    12961 caatattctt gtcgctgttg cggttacacc ggaaagtcag caactgctgg caaaagcggt
    13021 atctatcgcc aggccagtaa agggacacat cagtttaatt actctcgctt ccgacccgga
    13081 aatgtacaat caattagccg cgccgatgct tgaagattta cgcagcgtga tgcaagaaga
    13141 aacgcaaagc tttcttgata agttaattca ggatgcgggg tatcccgttg acaagacgtt
    13201 tattgcctac ggtgagttaa gcgaacatat tctggaagta tgtcacaaac atcatttcga
    13261 tttggtgatt tgcggtaatc acaatcacag tttcttttcg cgagcatcct gctcggcgaa
    13321 aagagtgatt gcctcaagtg aggtcgatgt gctgttagtt ccacttacgg gtgattaacc
    13381 gctcctacgc aagctttgga aaggtagcaa ctttatcgcg ctgctggctt tccgcgcttc
    13441 gcggaactat ctgctttagg tcgctaatga agcactcctg ccagtggtta atatcgtttt
    13501 tcacgataac gtccagcatt tctgcatgac gggaaatacg ttccgccagc gacatagtca
    13561 atgcacgatc cagcgcagct gcaacttcgt cacgatcgta ggggttaaca attaacgccg
    13621 acgttaactc gtttgccgct cccgcaaatt gcgaaagaac aagaacgccc gggttggctg
    13681 ggtcctgagc agcaacatac tcttttgcta ccaggttcat cccgtcacgc agtggcgtca
    13741 ctaagcccac gtcagagtag cggaatattt tcatcagtaa tttacggtca aaatgctgat
    13801 tcaaataata aagcggcgtc cagcctaatt gcccgtattt accattaatt cgtccagctt
    13861 cattttcgag ctgatgacga atatcctgat aggcttgcac atcaccacgc gacgttggtg
    13921 caatctgggt atgaagtg
//
LOCUS       DAJTMC010000103        13720 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.103, whole genome shotgun sequence.
ACCESSION   DAJTMC010000103 DAJTMC010000000
VERSION     DAJTMC010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13720
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.103"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(89..775)
                     /locus_tag="OO130_002757"
     CDS             complement(89..775)
                     /locus_tag="OO130_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HCR5366098.1"
                     /translation="MSFMAFVTCLTIAFIAGFAVARKLTLRKVASSVIIAKSQPVPSS
                     ATSPAAKASTRSYIPQKTYRDSRWQERDELFQGMVAEGILLERKEWLITRCEKMYFDN
                     LEKWFGHYCHIHCQVSLGRLINFPEQPEFSEEERKRFFAIYNGMAMDYVLVSKKNNKI
                     VCVIELDDPSHERADRIIRDRWLDTIMTLARVPFVRASVTKLEEIPPVWETRDKIRPL
                     VAATKSEPVH"
     gene            complement(1356..2144)
                     /gene="phoH"
                     /locus_tag="OO130_002758"
     CDS             complement(1356..2144)
                     /gene="phoH"
                     /locus_tag="OO130_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HCR5366099.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMSGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(2766..4037)
                     /gene="efeB"
                     /locus_tag="OO130_002759"
     CDS             complement(2766..4037)
                     /gene="efeB"
                     /locus_tag="OO130_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HCR5366100.1"
                     /translation="MQYKDENGVNEPSRRRLLKVIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPFDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHA
                     ARSKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFSLPGVKDANDYLGSALLRV"
     gene            complement(4043..5170)
                     /gene="efeO"
                     /locus_tag="OO130_002760"
     CDS             complement(4043..5170)
                     /gene="efeO"
                     /locus_tag="OO130_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HCR5366101.1"
                     /translation="MTINFCRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYAEQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITAQAEDLAQLRGVLGLD"
     gene            complement(5228..6056)
                     /locus_tag="OO130_002761"
                     /pseudo
     CDS             complement(5228..6056)
                     /locus_tag="OO130_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
     gene            complement(6725..8233)
                     /gene="putP"
                     /locus_tag="OO130_002762"
     CDS             complement(6725..8233)
                     /gene="putP"
                     /locus_tag="OO130_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HCR5366102.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALWAGAAATILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKHASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQES"
     gene            complement(8392..8601)
                     /locus_tag="OO130_002763"
     CDS             complement(8392..8601)
                     /locus_tag="OO130_002763"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5366103.1"
                     /translation="MMLQFCATMLNVTCVASLKMNEMLIKEIDMTGIKKITQTFSLRQ
                     LTFLKGATAKNVRECNLMKNSVAEH"
     gene            8656..12618
                     /gene="putA"
                     /locus_tag="OO130_002764"
     CDS             8656..12618
                     /gene="putA"
                     /locus_tag="OO130_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HCR5366104.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNCNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLENLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLVDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTYETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQPVAAGEMSPVINPAEPKDIVGFVREATPR
                     EVEQALESAVNNAPIWFATPPAERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGQGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQCNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVVDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRPVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELHKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGIQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSAVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            complement(12658..13296)
                     /gene="rutR"
                     /locus_tag="OO130_002765"
     CDS             complement(12658..13296)
                     /gene="rutR"
                     /locus_tag="OO130_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HCR5366105.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            13584..>13720
                     /locus_tag="OO130_002766"
     CDS             13584..>13720
                     /locus_tag="OO130_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HCR5366106.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     AL"
BASE COUNT         3406 a         3733 c         3376 g         3205 t
ORIGIN      
        1 tttaaagaag agctcgtacg aaggtacggg ctttttttcg tatattgcac acaccggggg
       61 gatgagaagc cccgaccggg atacttagtt aatgcaccgg ttcactcttt gtcgccgcga
      121 ctaatgggcg aattttgtca cgagtctccc agaccggagg aatttcctcc aatttagtga
      181 cagaagctcg tacaaaaggc acccgtgcaa gagtcataat tgtatccaac caacggtcac
      241 gaataatgcg atccgccctt tcatgactcg ggtcatccag ctcaataaca cagactattt
      301 tattgttctt ctttgaaacc agaacataat ccattgccat gccattataa attgcaaaga
      361 aacgctttct ttcttcttcg ctaaactcag gttgttctgg aaagttgatc aagcgcccca
      421 gagagacctg gcaatggata tgacaataat gtccaaacca tttttccaga ttatcgaaat
      481 acattttttc acaacgagtg attaaccact cttttctttc caataaaata ccttcggcaa
      541 ccattccctg aaataactca tctcgctctt gccaacgaga gtcacgatag gttttctgcg
      601 gtatatagct ccttgtcgac gccttagcgg caggggaagt tgcagatgaa gggacgggtt
      661 gcgatttagc gatgattacc gatgaggcaa cttttcggag tgtcaatttt ctggcaacag
      721 caaaaccggc gataaatgca attgtcaaac aagttacaaa agccataaag ctcataatta
      781 attttcacct atccatgttg attatatttc attaccaatg ttactcgctg cagcgtgtta
      841 catgccaaca atgcaacgat caatatatag ttagtatcat gttttgatta ttacaaccat
      901 ccctatttag ccattgcggc ttgccttcca caaaaaaata acaacacaca atattagcaa
      961 agaattaatt aagattaacc ttacatttat aatattatcc ctaaagaata aatcagagga
     1021 atatattcgg tgttaaacag tcagtcaaag tatagcccat tgttgattaa acctcataag
     1081 caaaattggc accagatctc ctgtacaggt tgaatggccg aaaaaagata ggcagatgct
     1141 catttttttg gcttctggaa gaaaaactct ccgcgctggc agtattacac tggtctgatg
     1201 ctatgacgaa aaatgcctgg taagagtatc taaaccagac tccagttcag cttatcaaca
     1261 cgctcaaagt cgcccccctg aacgctaacc tgaagtgaaa atctgttgaa ataaaacaaa
     1321 gcccggttcg cccgggctct gcaccgataa cacacttaac tgtatgcatg cagcgtacgt
     1381 tggcaaagtg ccgaacgtac gcaatcctct tttccgaacc ggacgatccc aaccatttca
     1441 tcttcttcga aacgttccag cgcgtcactt aatccggagc acacgccgcg aggcaaatcg
     1501 cattgcgtga tatcaccgtt gacgataacc gtcacgttct ccccgaggcg ggttaaaaac
     1561 attttcattt gcgcggcagt cacattctgc gcctcgtcaa gaatgacgac tgcattttca
     1621 aaggtacgtc cacgcatata ggcgaacggc gcgatttcca ccttccctat ttccggtcgc
     1681 aggcagtact gcataaagga agcccctaag cgccggacca gcacgtcgta gaccgggcga
     1741 aaatagggag caaacttttc tgcgatatca ccaggtaaga agccaagatc ttcatcggct
     1801 tgcagaactg gacgggtgac gataatcctg tcgacatcct tatgtatcag ggcctctgcc
     1861 gcttttgctg cgctgatcca ggtttttccg cacccggctt cgcccgtggc gaatatcagc
     1921 tgcttactct caatagcctt cagatagtgc aattgcgctt catttcgcgc gaggatgggc
     1981 gaggtatcgc gactgtcgcg ggccatacca atggcttcta cgccgctcat ctgcacaagc
     2041 gaggtgaccg attcttcttc acgctgctta tggctgcgcg aatcccgtct cagcacacgt
     2101 tttgcctcgc gacgagcttt gatcactgct ttttgtcttc ccatggagag caccttgagt
     2161 tgtttgtatt catcacacgc gccgttggca gcgcgattat gcgcacgaac atcagagggt
     2221 tggcttcctt gtaagccata gtttgctttt ggataaaatg ccgaaaaacg gctacgcgca
     2281 ccgtttacgg cgtcggtaac acatgaaaag aaaggatgag gttgaaaatg caaagtgacg
     2341 agatgactac cggaggagaa aactccgcga gtggtggcgc gttgattatc taaaacatgt
     2401 ccagtacagg acgttaccat ccgcgatctc catagtgact gactatcact gccgggaact
     2461 tccgctgcta cttaataagt acaacagatc tcgcatttta ttgcaacaat atatttactt
     2521 atatttaact ataaaacact atttcagtga cattagtttc tactggaaag atgacagagt
     2581 gatgacagtg atgaaaaaag ctgtgtgctt tcagcaggat ttgcatcagg catcagtgca
     2641 cgatcgccgg atgcggcgtg aacgccttat ccagcctacc aaacccgcac tgattgtagg
     2701 catgataaga cgcgcaagcg tcgcatcagg catctgcgca caaatgcctt atccgcctaa
     2761 aaacattaaa cccgcaataa cgcgcttccg agataatcgt tcgcgtcctt cactcccggc
     2821 agcgaaaaaa aataaccgcc gccgataggt ttaacgtatt cctccagcgc ttcgccattg
     2881 agcctttttt gtactgtcag gaagcctttt tccagatcgt gttggtagca gacaaacagc
     2941 aaccccatat ccagttgtcc ggagttggtg acccccaacg aataactgta gccgcgacgc
     3001 agcatcaggc tggactcact ctccgccgtg cggggattcg ccagccggat atggctgtcc
     3061 agcgcgatca ccttcccttc cgggtcgctg gcgtaatcag gcacatcatg ctcatgctgc
     3121 attcccagcg gcgcaccggt ttgcttatca cggccaaaaa tcgtctgctg ttctttcagc
     3181 ggcgttctgt cccaaaattc cactcgaaac tgaatcaagc gtactgcctg atagctgcca
     3241 ccgattgtcc acgcaggctc ctgctgatct gccgttaccc acaccacttt ttgcatcaac
     3301 ttatcattct ggctatcggg attggcagtg ccgtctttaa agcccagcaa attaatcggc
     3361 gtctctttgc ctttactacg cgctgcgtga tcggaaataa acccttcccg cttccagcgc
     3421 acgctgagca aatccggcgt gtgtttgatg atatcgcgca gcgcatggat caccgtgtcc
     3481 tgggtgttgg cgcaaatttg tagcaacaca tcaccatggc ataacgccgc atccagcgag
     3541 tcgttgggga aacgcgtcat cttctgcaac tttttcggca tctgtggcgc aaggccaaac
     3601 cgctcatcaa acaatgagtg acccaccgat aacgtgatgg tgagattatc gggcgcaatg
     3661 tagccgccaa gaatgccgga atcgaatggt ggcaggcgcg gatttggcgt ttctggtgcc
     3721 gctccgccct gagtcagaaa agcaaaacgc tgagtcaaca agcgaaacaa ccgttcaaga
     3781 tcggctttat cgctggcaag cacatcaaac gccaccagca tcattgcggc ctgttgtggc
     3841 gtcaggatcc ctgcctgatg ctcaccataa aacggctgtt tctcattgcg tgcatccggt
     3901 gaaagcgtac ccggcgcact ttgcgttttt tgtgcatgag cgaccggaca acttcccgcc
     3961 agcgccagcg cacctatcac tttcagtaaa cgtcggcgtg acggttcatt cacgccgttt
     4021 tcatctttat actgcataac gcttaatcca gtcccagcac accgcgaagt tgcgccagat
     4081 cttccgcctg cgcagtaatc ggtcctttca gtgcattccg gtcggcatcg gtcaatttgt
     4141 cgtaggtttc aaaaccgtct ttagtacggt atttcgccag aatggtatcg acctttttaa
     4201 agttggcatc gacttttgcc agcagttccg ggttggcttt ttgcagttgt ggacgcagca
     4261 aatcgacaat tttctgcgag ccttcaacgt tagcctggaa atcccacaga tcggtgtggc
     4321 tgtagcgatc ttcttcaccg ctaattttgc tggctgccac ttcctcaatc agtccggctg
     4381 cgccgccgac cacttttgaa ggtgggaaag ccagttcact gatgcgtttt tgcaaatcga
     4441 ccacatctgt ataaagctgc tcagcgtact gatccatccc tttggtggtg ttgtcgccaa
     4501 acaatgcttt ttccagacgg tggaaaccag tgaattttgg gtcggcggct ttttgctcgt
     4561 aatcatcttc acgggcgtca atgctgccat ccagatcgga gaacagttca gcaatcggtt
     4621 caatgcgctc atagtgctgg cgcgtcggcg catacagtgc tttcgctttt tcgatatcgc
     4681 cagctttaat cgcgtcggta aaggctttgg tgtcggtcac cagctgcgtg gtttccgcca
     4741 tgacatacgc tttatatgca gtaattgcac caccaagact taacagcgca tcactttgcg
     4801 ccgcatccgc cgttgcctca cctttgacga tcaacttccc tttcgggtta gtcagcagac
     4861 cgcaggtcat atcgtattcg ccaggctgta aattcgccgt cattttctgg ctaaaaccag
     4921 gggcgatatt ttcccgctct tccaccacca tcacgccttt gaggatctcc cactccagcg
     4981 ccttctggct atggttctga ataatgaact gtgttttccc ggcgttaacc gtaatggtca
     5041 tcggttcgca ctgcttatcc gtcacggtca ctttgacctg cggcacatca gcggcgttag
     5101 ccataaaagc agaagaaaac agcgcagcca cgctcaactg caatgcgtta cggcagaagt
     5161 taatggtcat gacgagacta tccctttaaa gtatgttgta actaagcaag agtttgcgtc
     5221 gtatttgtta cgcggagcga gacgctgtcg cccctgcgcg tggtggcata gcaaatgcca
     5281 ccagcgccgg gatgagataa ataaaccaga cggcgacttc gctgacgctc ggcgcttcct
     5341 gatagccaaa aatcccttcc atcagcgtgc caaacagcga gtgagttgag agcaccgcac
     5401 tcatatcgaa ggcgatttcc tgaaagtggt tccacaatcc ggcttcatga aatgcgcgaa
     5461 tggcaccagc tgccagccct gcggcgacga agagaataaa caggctggtc catttaaaaa
     5521 atgcaccaag attgaggcga ataccgcccc agtagagcag aaagccgagc accacggctg
     5581 tagcaagacc gagcattgca cccagcggcg gccagatccc gacatcttgt tgaaatgccg
     5641 ccagcaggaa aaagaccgac tccagccctt cccttgcaac ggcaaaaaag accatcatca
     5701 ccagcgccca gccatgatga tttccacgct acaatgcgct atcgactgcc tgttccagtt
     5761 gcacttacgt tgcgcgacac tttgcgcatc cagaaaacca tccaggtaag gatcaccacg
     5821 gcgatcaccg ccacgatacc ttcaaacagt tcctgttctt tttgcggaaa ttcgccggtg
     5881 gtttcgttaa tgaagatccc caggcccagg cacaacgcag cggcaagcaa cacgccaatc
     5941 cacatcacac caatccatcg gcctcgctgg gtacgcttaa gatagctggc aatcaaactg
     6001 acaatcagcg cggcttcaag tccttcgcgc aacataatga gaaacggaac aaacatgcca
     6061 cccaccctta aacgtttttc tgagtcaatc tatgcaaaga aaagtaaatc gcagtgatag
     6121 cgattatcat tacggtaaag aaaaatacaa gcggaatgtt tataaaatga tgttggaatg
     6181 taaacaaaga gaggtaatgc caggggatat aaacgattat tccagggtag aaagatggga
     6241 ttgtttgcaa agaaccatgt gttcttgccg gatgccgcgt aaacgccata tccagccgac
     6301 aaaactgtcc agactcaatg catcagagct tccatgtaaa actgataagc gggtgtatca
     6361 agcaatttcg tccatccgcg attatcaaat ctaacttttt gcccggaaca taataacagc
     6421 tcgggtgttg gtggcgtcaa aatcgctgaa gcgttccccg aaggccggga cagcccacat
     6481 ggatgtgggc tgagggcgcg tcttacagga cgttacctcg cgcccgaccc ggtagccgaa
     6541 ggggataagc tgaaggtacc gcgcagcggc gattttgttc gccagagccc gggggtgcac
     6601 agggggcggc ggcgattggc cgccccctgc gcgctccctg caccattcaa tttatatggc
     6661 gaggcacatt aagggagcgc aacccgctgc tgaagccaaa caaaaccgcc gcaggctaag
     6721 tcccttagct ttcctgcaac cgtgacggcg gagccgaatg atagtgcgca tcggcctcgg
     6781 caaagcgttt ttgcatcgcc gctgacggcg ctttacccag caaactaaac actacaatcc
     6841 cgatactgcc gaagatgaag cccggaataa tttcgtacag tcccagccag ccgaactgct
     6901 tccagacgat aaccgtcagc gcaccgatga tcatccccgc cagtgcaccg ttacgcgtca
     6961 tgcgtgacca catcaccgag aacagcacca ccggaccaaa cgccgcgcca aagcctgccc
     7021 acgcgtagct cactaagccc agcacgcggt tttccgggtt tgccgccagc gcaatcgcca
     7081 ccagcgccac caccagcacc atcacacgcc ctacccacac cagctctttc tggctggcat
     7141 gtttacgcag aaacgctttg tataaatctt cggtaatcgc actggagcac accagcagtt
     7201 ggcaacttaa ggttgacatt accgccgcca gaattgccga cagcagaatc ccggcaatcc
     7261 acgggttaaa cagaatttgc gccagttcga taaacacgcg ctcggcgttc tggtttaccg
     7321 cgcctgctac cgccgggtgc tcattaaagt aagcaatccc aaagaagccg acagccaccg
     7381 cccctgccag gcagaggatc atccaggtca tactgatacg acgcgcatgg acaatgctgt
     7441 ggtgagaatc cgccgccata aaacgcgcca gaatgtgcgg ctgcccgaag taacccagcc
     7501 cccaacccat cagtgagata atggcaacaa agttcagacc tttgagcata tcaacgtttt
     7561 cgatgctctt ttgtttgatc acttccagcg agtcaccaaa gccaccgaca ctgataatga
     7621 cgataaccgg tgtcaggatc agggcaaaaa tcatcaggct ggcctgtaca gtgtcagtcc
     7681 agctcaccgc gaggaaaccg ccaataaagg tgtaaaggat cgtcgccgca gctcccgccc
     7741 acagagccgt ttcgtagctc atgccaaagg tactttcaaa cagacgcgcg cctgccacaa
     7801 tgcccgaagc gcaataaatg gtgaagaaca gcaaaataac cagcgcggag ataatgcgca
     7861 aaatgcggct tttatcttca aaacgcccgg tgaaataatc cggcaatgtt aaggcgttat
     7921 tattgtattc ggtatgcaca cgcaaccgcc cggccaccag cttccagttg atccacgcgc
     7981 ctaatgtcag gccaatggcg atccagcttt cggaaatccc ggaaagaaaa accgcgccag
     8041 gcaaccccat taacagccag ccgctcatat ccgacgcacc cgccgataat gccgtcacaa
     8101 atggcccaag actacgaccg cccagaatat agtcgtcaaa gtttttcgtt gatcgccagg
     8161 cgataaaccc aatcaatatc atgccaaaga tatagacaca aaatgtcacc aacatcggtg
     8221 tgctaatagc catctaaagt ctccaaaaaa ttattatcgg caatgtcgaa acttgccgtt
     8281 atatctgcaa ccggaacggg gtaacagagt ttatttttta ccagggcgac cgtatcctgc
     8341 cggaagcgct ggttattcac aatcgattta acacaccatt tacattaaat tttagtgctc
     8401 agcgacacta tttttcatca ggttgcactc tctcacattt tttgcggttg cacctttcaa
     8461 aaatgttaac tgccgcagag aaaaagtctg agttattttt ttaatccctg tcatatcaat
     8521 ttcttttatt aacatttcat tcatttttaa gcttgctacg caggtcacat ttaacatggt
     8581 tgcacaaaat tgcaacatca tggatatttc acgataacgt taagttgcac ctttcagaac
     8641 aacaggagta atggcatggg aaccaccacc atgggggtta agctggacga cgcgacgcgt
     8701 gagcgtatta agtctgccgc gacacgtatc gatcgcacgc cacactggtt aattaagcag
     8761 gcgatttttt cttatctcga acaactggaa aacagcgata ctctgccaga gctacctgcg
     8821 ctgctttctg gtgctgccaa tgagagcgat gaagcaccga ctccggcaga ggaaccacac
     8881 cagccattcc tcgactttgc cgagcaaatt ttgccccagt cggtttcccg cgccgcgatc
     8941 accgcggcct atcgtcgccc ggaaaccgaa gcggtttcta tgctgctgga acaagcccgc
     9001 ctgccgcagc cagttgctga acaggcgcat aaactggcat atcaactggc cgataaactg
     9061 cgtaatcaaa aaaatgccag tggtcgcgca ggtatggtcc aagggttatt gcaggagttt
     9121 tcgctgtcat cgcaggaagg cgtggcgctg atgtgtctgg cggaagcgtt gttgcgtatt
     9181 cccgacaaag ccacccgcga cgcgttaatt cgcgacaaaa tcagcaactg taactggcag
     9241 tcacacattg gtcgtagccc gtcactgttt gttaatgccg ctacctgggg gctgttgttt
     9301 actggcaaac tggtttccac ccataacgaa gccagcctct cccgctcgct gaaccgcatt
     9361 atcggtaaaa gcggtgaacc gctgatccgc aaaggtgtgg atatggcgat gcgcctgatg
     9421 ggcgagcagt tcgtcacggg tgaaaccatc gcggaagcgt tagccaatgc ccgcaagctg
     9481 gaagagaaag gtttccgtta ctcttacgat atgctgggcg aagctgcact gactgccgca
     9541 gacgcacagg cgtatatggt ttcctatcag caggcgattc acgccattgg taaagcgtct
     9601 aacggtcgtg gcatctatga aggtccgggc atttcaatca agctgtcggc gctgcatccg
     9661 cgctacagcc gcgcccagta tgaccgggta atggaagagc tttacccgcg tctgaaatca
     9721 ctcaccctgc tggcgcgtca gtatgatatt ggtattaaca tcgatgccga agaggccgat
     9781 cgtctggaga tctccctcga tctgctggaa aatctctgtt tcgagccgga actggcaggc
     9841 tggaacggca tcggttttgt tattcaggct tatcaaaaac gctgcccgtt ggtgatcgat
     9901 tacctggttg atctcgccac ccgcagccgt cgccgtctga tgattcgcct ggtgaaaggc
     9961 gcgtactggg atagtgaaat caagcgtgcg cagatggacg gccttgaagg ctatccggtt
    10021 tatacccgca aggtgtatac cgacgtttct tatctcgcct gtgcgaaaaa gctgctggcg
    10081 gtgccgaatc taatctaccc gcagttcgcg acgcacaacg cccatacgct ggcagcgatt
    10141 tatcaactgg caggtcagaa ctactatccg ggtcagtacg agttccagtg cctgcatggt
    10201 atgggcgagc cactgtacga gcaggtcaca gggaaagttg ccgacggtaa acttaaccga
    10261 ccgtgtcgta tttatgcccc ggttggcaca tatgaaacgc tgctggcgta tctggtgcgt
    10321 cgcctgctgg aaaacggtgc taacacctcg tttgttaacc gtattgccga cacctctttg
    10381 ccactggatg aactggtcgc cgatccggtc actgctgtag aaaaactggc gcaacaggaa
    10441 gggcaaactg gattaccgca tccgaaaatt cccctgccgc gcgatcttta cggtcacggg
    10501 cgcgacaact cggcagggct ggatctcgct aacgaacacc gtctggcctc gctctcctct
    10561 gccctgctca atagtgcact gcaaaaatgg caggccttgc caatgctgga acaaccggta
    10621 gcggcaggtg agatgtcgcc cgttattaac cctgcggaac cgaaagatat tgtgggcttt
    10681 gtgcgtgaag ccacgccgcg tgaagtagaa caggcgctgg aaagtgcggt taataacgcg
    10741 ccaatctggt ttgccacgcc tccggctgaa cgcgcggcga ttttgcatcg cgctgccgtg
    10801 ctaatggaaa gccagatgca gcaactgatt ggtattctgg tgcgtgaagc cggaaaaacc
    10861 ttcagtaacg ctattgccga agtgcgcgaa gcggtcgatt ttctccacta ctacgccgga
    10921 caggtgcggg atgatttcgc taacgaaacc caccgtccat tagggcctgt ggtgtgtatc
    10981 agtccgtgga acttcccgct ggctattttc accgggcaga tcgctgccgc actggcggca
    11041 ggtaacagcg tgctggcaaa accggcagaa caaacgccgc tgattgccgc gcaagggatc
    11101 gccattttgc tggaagcggg tgtaccccct ggcgtggtgc aattgctgcc aggtcagggg
    11161 gaaaccgtgg gcgcgcaact gacgggtgat gatcgcgtgc gcggggtgat gtttaccggt
    11221 tcaaccgaag tcgctacgtt actgcagtgc aatatcgcca gccgcctgga cgctcagggt
    11281 cgccctattc cgctcatcgc tgaaaccggc ggcatgaacg cgatgattgt cgattcttca
    11341 gcactgaccg aacaagtcgt cgtggatgtg ctggcctcgg cgttcgacag tgcgggtcag
    11401 cgttgttcgg cactgcgcgt gctgtgcctg caagatgaga ttgccgacca cacgttgaaa
    11461 atgctgcgcg gtgcaatggc cgaatgccgg atgggtaatc cgggtcgcct gaccaccgat
    11521 atcggtccgg tgattgatag cgaagcgaaa gccaatattg agcgccatat tcagaccatg
    11581 cgtagcaaag gccgtccggt gttccaggcg gtgcgggaaa acagcgaaga tgcccgtgaa
    11641 tggcaaagcg gcacctttgt cgccccgacg ctgatcgaac tggatgactt tgccgaattg
    11701 cacaaagagg tctttggtcc ggtgctgcat gtggtgcgtt acaaccgtaa ccagctacca
    11761 gagctgatcg agcagattaa cgcttccggc tatggtctga cgcttggtgt ccatacgcgt
    11821 attgatgaaa ccatcgccca ggtcactggc tcggcccatg ttggtaacct gtatgttaac
    11881 cgtaatatgg tgggcgcagt ggttggtgtg cagccgttcg gcggcgaagg gttgtccggt
    11941 accgggccga aagcaggcgg tccgctctat ctctaccgtc tgctggcgaa tcgcccggaa
    12001 agtgcgctgg cagtgacgct cgcgcgtcag gatgcagagt atccggtcga tgcacagttg
    12061 aaagccgcat tgactcagcc gctaaatgca ctgcgggaat gggcagcaaa tcgtccagaa
    12121 ttgcaggcgt tatgtacgca atatggcgag ctggcgcagg caggaataca acgattgctg
    12181 ccggggccga cgggtgaacg caacacctgg acgctgctgc cgcgtgagcg cgtgttgtgt
    12241 attgccgatg atgagcagga tgcgctgact cagctcgccg ccgtgctggc ggtgggcagc
    12301 caggtactgt ggccggatga cgcgctgcat cgtcagctag taaaggcatt gccatcggca
    12361 gtcagcgaac gtattcaact ggcgaaagcg gaaaatataa ccgctcaacc ctttgatgcg
    12421 gtgatcttcc acggtgattc ggatcagctt cgcgcattgt gtgaagccgt tgctgcgcgg
    12481 gatggagcaa ttgtttcggt gcagggtttt gcccgtggcg aaagcaatat ccttctggaa
    12541 cggctgtata tcgaacgttc gctgagtgtg aataccgctg ccgctggcgg taacgccagt
    12601 ttaatgacga tcggttaaac aggtagccgg aggatgttac accctcctcc ggcatcttta
    12661 acgtggtcga atcccctcaa taataatccg ctgcacgttt tcaaccgttt gattgaaaaa
    12721 tacctcatcg cgcaacgttg cgcctgtcac cgcctccacc tgaggggcga aatcggcgta
    12781 atgttgagtg gaagcccaaa tcataaaaat caaatgctgc ggatcaatcg gcgcgagttt
    12841 gccgcttttg acccaaccgg caatcagcgc cgatttctca tcaattaatg ccttcaaatc
    12901 gcccgtcagt tcatccatta acagcggcgc gcctgccagc atctccatac agaacaggcg
    12961 cgaagcctgc ggataatcgc gtgagacttc cagcttcaga cggatgtact ctttgatcgc
    13021 cgccagcggg gcgaaatctt cacgaaacgc ttttaacggt gccagccaga tatcgagaat
    13081 ctgccgcagc acggcaatat acagcgcctc ttttgacgga aagtaataca gcagattggt
    13141 ttttgaaaca cccgccaact ctgcgatctg ctccagcctt gtgccgtgaa aaccgaattg
    13201 tgaaaaagtg tccagtgctg cgctaagaat cgcttttttc ttcgcgctta ctgcgcgcga
    13261 acgtttaccc gttgttttca ctgcgccttg cgtcatgcgc tctcccctct tgtagattgg
    13321 caacaggata gcaaaacccc cgcccggcct cgaccttctg cactctcatc gcgcacggat
    13381 gcatgtttta tgtgcaactg ttttgaccgt ttagtccact ttttaccaga tattttagat
    13441 aacacaatgc taacacatta ataacattca ctttttaaaa actggcatcc gctttgcaaa
    13501 caagccaata cgcggctgaa aaggatatgc aggatgcagc gccccgtctg actttcactt
    13561 tacgagacga agagaggttg atgatgaaaa ttggcgtatt cgtacctatt ggcaacaacg
    13621 gctggctcat ttcgacccac gcgccgcagt acatgccgac ctttgaactg aataaagcca
    13681 ttgtgcaaaa agcggagcat taccatttcg atttcgccct
//
LOCUS       DAJTMC010000104        13677 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.104, whole genome shotgun sequence.
ACCESSION   DAJTMC010000104 DAJTMC010000000
VERSION     DAJTMC010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13677
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.104"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            1..679
                     /gene="csgG"
                     /locus_tag="OO130_002767"
                     /pseudo
     CDS             1..679
                     /gene="csgG"
                     /locus_tag="OO130_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
     gene            complement(743..1234)
                     /locus_tag="OO130_002768"
     CDS             complement(743..1234)
                     /locus_tag="OO130_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HCR5366107.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(1336..1890)
                     /gene="ycdY"
                     /locus_tag="OO130_002769"
     CDS             complement(1336..1890)
                     /gene="ycdY"
                     /locus_tag="OO130_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HCR5366108.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMAQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWMEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(1914..2651)
                     /gene="ycdX"
                     /locus_tag="OO130_002770"
     CDS             complement(1914..2651)
                     /gene="ycdX"
                     /locus_tag="OO130_002770"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HCR5366109.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(2706..3644)
                     /gene="ghrA"
                     /locus_tag="OO130_002771"
     CDS             complement(2706..3644)
                     /gene="ghrA"
                     /locus_tag="OO130_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HCR5366110.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLNPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHWEDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVEYISRTIAQLEKGERVCGQVDRARGY"
     gene            3878..3965
                     /locus_tag="OO130_002772"
     tRNA            3878..3965
                     /locus_tag="OO130_002772"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3912..3914,aa:Ser,seq:gga)
     gene            complement(4115..4957)
                     /locus_tag="OO130_002773"
     CDS             complement(4115..4957)
                     /locus_tag="OO130_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HCR5366111.1"
                     /translation="MSDITISRPEVVNGHTDVICSTSIRHILAVRKSTLLQIDTLIRQ
                     LAEISVLTESIGGKTAPDWAMKQDFRCGCWLMEKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDTQARDTWYRSLEYDNFPEISEANILSTFEQLHQNKDEVFERGVINVFRGLN
                     WNYKTNCPCKFGSKIIVNNLVRWDRWGFHLITGQQADRLADLERMLHLFSGKTIPDNR
                     GNITLRLDDHIQSVQGKESYEDEMFSIRYFKKGSAHITFRKPELVDRLNDIIAKHYPD
                     MLAV"
     gene            complement(5042..5239)
                     /locus_tag="OO130_002774"
     CDS             complement(5042..5239)
                     /locus_tag="OO130_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5366112.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCGSGIIFDNDEDKTDSAALLPC
                     IEQAREDVRTLRHLQLLHQNR"
     gene            complement(5251..5742)
                     /locus_tag="OO130_002775"
     CDS             complement(5251..5742)
                     /locus_tag="OO130_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309434.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5366113.1"
                     /translation="MMKLALTLEADSVNVQALNMGRIVVDVDGIELAELINMVCDNGY
                     SLRVVDESDQTSAECTPPFATLTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHF
                     TGSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAECLPGLPT
                     FDW"
     gene            complement(5739..6113)
                     /locus_tag="OO130_002776"
     CDS             complement(5739..6113)
                     /locus_tag="OO130_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HCR5366114.1"
                     /translation="MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFV
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKYPEAK"
     gene            complement(6160..6534)
                     /locus_tag="OO130_002777"
     CDS             complement(6160..6534)
                     /locus_tag="OO130_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HCR5366115.1"
                     /translation="MSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLYYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRYQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPAPATTS"
     gene            complement(6697..6918)
                     /locus_tag="OO130_002778"
     CDS             complement(6697..6918)
                     /locus_tag="OO130_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HCR5366116.1"
                     /translation="MRIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(6981..7457)
                     /locus_tag="OO130_002779"
     CDS             complement(6981..7457)
                     /locus_tag="OO130_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HCR5366117.1"
                     /translation="MQQLSFLPGNMTPGERSLILRALQTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            complement(7473..7946)
                     /locus_tag="OO130_002780"
     CDS             complement(7473..7946)
                     /locus_tag="OO130_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HCR5366118.1"
                     /translation="MKTLSQNTTASACAPETGLQQLVATPVPDEQRISFWPQHFGLIP
                     QWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMNGN
                     RAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            complement(8288..9106)
                     /locus_tag="OO130_002781"
     CDS             complement(8288..9106)
                     /locus_tag="OO130_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HCR5366119.1"
                     /translation="MRLASRFGYANQIRRDRPLTHEELMHYVPSIFGEDRHTSRSKRY
                     AYIPTITVLESLQQEGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVEKVIEGAYEVVGVFD
                     RIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSAKGKRIHTRAIHNIDTDIKLNRALWVMAETLLESLR"
     gene            complement(9261..9419)
                     /locus_tag="OO130_002782"
     CDS             complement(9261..9419)
                     /locus_tag="OO130_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001550547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HCR5366120.1"
                     /translation="MPGCTSRLLPEGPFSREQAVAVKTAYRNVFTEDDQGTYSRLVIR
                     NAEGQLRW"
     gene            complement(9490..12336)
                     /gene="ag43"
                     /locus_tag="OO130_002783"
     CDS             complement(9490..12336)
                     /gene="ag43"
                     /locus_tag="OO130_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HCR5366121.1"
                     /translation="MKRHLNTSYRLVWNHITGTLVVASELARSRGKRAGVAVVLSLAA
                     VTSVPALAADTIVQAGETVNGGTLTNHDNQIVLGTANGMTISSGLEYGPDNEANTGGQ
                     WIQNGGIANNTTVTGGGLQRVNAGGSVSDTVISAGGGQSLQGQAVNTTLNGGEQWVHE
                     GGIATGTVINEKGWQTVKSGAMATDTVVNTGAEGGPDAENGDTGQFVRGNAVRTTINE
                     NGRQIVAAEGTANTTVVYAGGDQTVHGHALDTTLNGGYQYVHNGGTASGTVVNSDGWQ
                     IIKEGGLADFTTVNQKGKLQVNAGGTATHVTLKQGGALVTSTAATVLGSNRLGNFTVE
                     NGKADGVVLESGGRLDVLEGHSAQKTRVDDGGTLAVSAGGKATGVTMTSGGALIADSG
                     ATVEGTNASGKFSIDGTSGQASGLLLENGGSFTVNAGGLASNTTVGHRGTLTLAAGGS
                     LSGRTQLSKGASMVLNGDVVSTGDIVNAGEIRFDNQTTPDAALSRAVAKGDSPVTFHK
                     LTTSNLTGQGGTINMRVRLDGSNTSDQLVINGGQATGKTWLAFTNVGNSNLGVATSGQ
                     GIRVVDAQNGATTEEGAFALSRPLQAGAFNYTLNRDSDEDWYLRSENAYRAEVPLYTS
                     MLTQAMDYDRILAGSRSHQTGVNGENNSVRLSIQGGHLGHDNNGGIARGATPESSGSY
                     GFVRLEGDLLRTEVAGMSLTTGVYGAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLNL
                     THTSSGLWADIVAQGTRHSMKASSDNNDFRARGWGWLGSLETGLPFSITDNLMLEPQL
                     HYTWQGLTLDDGQDNAGYVKFGHGSAQHVRAGFRLGSHNDMSFGEGTSSRDTLRDSTK
                     HRVSELPVNWWVQPSVIRTFSSRGDMSMGTAAAGSNMTFSPSRNGTSLDLQAGLEARV
                     RENITLGVQAGYAHSVSGSSAEGYNGQATLNVTF"
     gene            complement(12708..13580)
                     /locus_tag="OO130_002784"
     CDS             complement(12708..13580)
                     /locus_tag="OO130_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HCR5366122.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVQRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILHRGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQAQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDELRTESVRGQAREQFTGAVDRIFDTAESVCVASVARTVLRAVRDT
                     VVSVARAVWNWIFF"
BASE COUNT         3198 a         3682 c         3549 g         3248 t
ORIGIN      
        1 ggtatacaac attcaggacg aaaccgggca atttaaaccc tacccggcaa gtaacttctc
       61 cactgctgtt ccgcaaagcg ccacggcaat gctggtcacg gcattgaaag attctcgctg
      121 gtttataccg ctggagcgct agggcttaca aaacctgctt aacgagcgca agattattcg
      181 tgcggcacaa gaaaacggca cggttgccat taataaccga atcccgctgc aatctttaac
      241 ggcggcaaat attatggttg aaggttcgat tatcggttat gaaagcaacg ttaaatcggg
      301 cggtgttggg gcaagatatt ttgggatcgg tgcagacacg caataccagc tcgatcagat
      361 tgccgtgaac ctgcgtgtcg tcaatgtgag taccggtgag atcctctcct cggtgaacac
      421 cagtaagacg atactttcct atgaagttca ggccggggtt ttccgcttta ttgactacca
      481 gcgcttgctg gaaggggaag tgggttacac ctcaaacgaa cccgttatgc tgtgtctgat
      541 gtcggctatc gaaacagggg tcattttcct gattaatgat ggtatcgacc gtggtctgtg
      601 ggatttgcaa aataaagcag aacggcagaa tgacattctg gtgaaatacc gccatatgtc
      661 ggttccaccg gaatcctgac agataaaaaa gccggaatta tcatccggca ttttactgtt
      721 taagcgaaag cgtgagtgcc cctcacgctt tatttttgat ttcctgctca cggtgagccg
      781 tctttgcact acgcgccgcc agccacaggc cgctgttttt cattgcgtaa ccaaatacca
      841 gcccaagcgc cagcgaaggt aacaccagtt tccagtcacc ctgcccggca aacgtcgcgc
      901 atgcgcctat aaacgtcccc ggaacaaatg aaagcagcag ctgtttggcc tgaatacaca
      961 tcagaaacgc cacaataccg gttatgacat aaccgagaat ttccagatgt ggtgccagtg
     1021 cactaccgta aataatgacc atcgcccaca ccacgccact aagcagggtt gcagcggaga
     1081 tcgccagccc tttcagccca ccttgcgggc aggcaaagta ggccgtacag cccaggaagc
     1141 ccgcccagct tagtaagccc agggaaacag ccacccatcc ccagataccg gagagaatgc
     1201 ctgttgtgat tgcaatagag agaagtatgt tcatgccgca catgatagca gaaaacgcgg
     1261 ccatgaatga gatcgagcac acattttatg caacaacaaa ttatctgttg cagttatgag
     1321 tgacgatgat cacatttact cttcagaatc ttcttccagc tcgtcccaca ttgcactaat
     1381 ggcatcacgg gttagcggtg ccatggtgcg ccagaaaggc gtggttgcat gggcctccac
     1441 tttgccgagg aacgcaccac accagggtaa cagatactca ctgaacagtg tttccagtgc
     1501 ttcgctctca tcttccgttg actgatcttc catccaggaa gccgcaagca gcaatgtgcc
     1561 gatgtgatcg gctggcgtat ccgccagtgg catccctcgt tcggaaagaa aagcgcgcac
     1621 ttccgcttcc gttgcgtcct caacccatgc gctacgatat ggcggcacgg cacattcatc
     1681 gccgataaac aacgcattgt aatcggcaga gacttgcgcc atatcacagc ttttctgtaa
     1741 acgcgtcagt aactcatcct gctccagtgg ccagttcgca gccagtttcc cctcacgaat
     1801 cagggtaaac agcggcacca gtaaaggatc ttgcggttgg cggtaataga gcgaacccag
     1861 cacacgacag aggatagaaa actcgttcat taatatattt ccattactag taattaaagg
     1921 tctgcaaatt ccgcaatcgg tgccataccg cgagattcaa ggaagttcag taagcggcgc
     1981 ggagaaacat tcaaaatgcg ctctggcgga aaatctaccg cgtcgaggat tttaagacac
     2041 tcttcaaatt cccccatggt aaacgcggtg tgagaatccg agcctaatgc cacccaacct
     2101 ccagcatcac gtaccgctgc ggctaccgca cgacagttgt cttcactgcc cttacgtgag
     2161 tgtaaaaatg aagaattatt gatttccagc gccacctgat gtttcgcggc tgcttcagca
     2221 acggctttca catctatttc atatttggga tttccgggat ggcttattat atgcacattg
     2281 ccgctggcga tagtggcgat catcgcttgt gtattggtcg ctttgtcatg tggtgcaaaa
     2341 accggctcat gaaaaccggc aataatcaga tccagcgagt caaacatttt accgctgcaa
     2401 tcaatttcac catcaacatt tttaatgtta gcttcgatgc cgcgcaggat ccctacccca
     2461 tcaaccactc gcggccagat acgcatgtta atgaagtgcc agtgatgcgg agcatcttcc
     2521 atatccgggc catgatcggt gatcgcaaaa agtttaatgc ccttttgttt ggcctgggca
     2581 atgtaatcac tcaatgtgct atatgcatgt gtgctggcaa cggtatgcat atgaaggtcg
     2641 acgggataca taactctctc ctgtattcaa ttcccgccaa ggatagcagg aatcctgatg
     2701 ctttattagt agccgcgtgc gcggtcgact tgcccgcaga ccctctcccc tttttcgagc
     2761 tgggcaatgg tgcgagaaat gtactccaca gcttcagcgg gacgggtaat cgcggcgaca
     2821 tgtggtgtta tcgtcacgcg tggatgttgc cagagcggac tttcaggcgg taagggttca
     2881 cgattaaaaa catccaacat tgcgccttta actttgccgc tatccagcgc cgcgagcagg
     2941 tcatcttcca caacatgaac accacgcgcc aggttgagga gatacgcgcc atccggtaat
     3001 ttttcgagta attgttgatt aataatgccg acggtttcag gggtattcgg taacaaatta
     3061 atcaataccc gacattggct cagaaatgca gacagttctt cccgtccggc aaagctttgc
     3121 acgccaggcc acgatttacg ggttcgactc cagcaacgca gcggaaagcg ccaggtttgc
     3181 agactctgag caactttact gcccagtacg cctgcgccca aaatgccgat ggtaaaatct
     3241 tcccaatgat attcaggcag cggttgccaa tgcgaactat tttgctggat gcgataatcg
     3301 tcaaaacgtc gaaaccaatg cagcacctga ctgacagcat attcctgcat ttgctcgccc
     3361 ataccagtat cttccaggcg aaaaagtgga acagaagggt tcagcatttc agggtgtgcc
     3421 tgtagcttgc tcaaaataga atcaacaccg gccccgagtg cgaacaccgc tttaagatcg
     3481 cgccctgcca gcatttcaac aggaggatgc cagactaaag cataatcagc agagtcatta
     3541 tctccgcttt tccatgctct gactcttgcc tgaggaatag ctttgcgcag tgcctcaatc
     3601 caccattggg tatcgaacgt tgggtgataa aagatgatat ccatactgac tcccgaaaag
     3661 cgttgtgcga aatttattcg cacttatcgt tatgatctac aaaggccacc agcataacaa
     3721 atccgtggtc ggtggcaaaa aaagcagatt tcgcttatta aaaccacaca ttgattgaag
     3781 tttgaataaa cgcgcgattt tttcaaaaag tttgttgacc tcaggtcatg atttccctaa
     3841 attagcgccc gttccagcaa gacaggaaca acaatttggt gaggtgtccg agcggttgaa
     3901 ggagcacgcc tggaaagtgt gtatacggca acgtatcggg ggttcgaatc cccccctcac
     3961 cgccagatta taagcgcatg ttattgttta acatgcgctt ttttattttt ccttccagac
     4021 agtatgccat cgggtatgcc atctgaaacc ggtttatatg ccccgcgatc atatcttatc
     4081 ggacaatttc cgatctttga gcatactggc aaagctatac tgccagcata tcaggatagt
     4141 gttttgcgat aatatcattc agtctgtcaa ccagttctgg ttttctgaac gtgatatgcg
     4201 cggagccctt cttaaagtat ctgatgctga acatctcatc ttcatagctc tctttaccct
     4261 gaacagactg aatgtgatca tccagacgaa gggtgatgtt tccccggttg tcggggattg
     4321 ttttaccgct gaacaggtgc aacattcttt ccagatcggc gagtcggtca gcctgttgtc
     4381 cggtgataag atgaaatccc caccggtccc atctcaccag attgttgacg ataattttac
     4441 tgccaaattt acaggggcaa ttggttttgt aattccagtt cagccctctg aagacgttga
     4501 tcacccctcg ctcaaacacc tcatccttat tctggtgcag ttgttcaaat gtgctcagaa
     4561 tgttcgcttc gctgatttcc ggaaaattat cgtactccag tgaccggtac catgtatcac
     4621 gggcctgcgt atccattaat gacagcatcc cggaacgttg catcaggtca cgccagattt
     4681 cgcgatcaag attccgggta atggctttca ttgctgtttc gggtttctcc atcagccagc
     4741 agccgcagcg aaaatcctgt ttcatggccc agtccggggc agttttacct ccgatgcttt
     4801 ccgtcagcac cgagatttca gccagttgtc ggataagtgt gtcgatttgc aacagtgtac
     4861 tctttcgtac agccagaatg tggcgaatgc tggttgagca gataacgtcc gtatgcccgt
     4921 tgaccacttc cggacgggag attgtgatat ctgacataat cgatattcct ttttgcagat
     4981 aaatgaaaac gccagccctg tgcaggctgg cgtcagagga atgaagtctg gtgagtgagc
     5041 ttcaccggtt ctggtgcaga agctgcagat gacgcagggt acggacatcc tcccgggcct
     5101 gttcgataca gggcaacagt gctgctgaat ccgttttatc ctcatcgttg tcaaagataa
     5161 ttcccgatcc gcagtcgata ttgtcctgca gccacgcaat cagaatatcc agcgctgttt
     5221 ccgtggttaa tgatttcatg ttgttcctgg ttaccagtcg aaagtgggta aacccggcag
     5281 gcattcagcg ctggcatcca gatgaatgag actgacgcca taacgccgga tgagtgtgac
     5341 gaccagacga cggaacgttt ttgacagccc caggcgttta agccgcagaa ccgggtatga
     5401 ccacgcatcg gtacgtaaca gatatccgct acctgtaaaa tgaatccatt ctgattcacc
     5461 gaagtcactg gtctggtgtg acagcgagta caaccaggcg ttgtccgttt ccgtgatatg
     5521 tgcggtactg cagcgtatgc cggtaagggt agcaaatggt ggcgtgcatt ctgctgaggt
     5581 ctggtcagat tcatcaacaa cacgaaggga gtagccgtta tcgcagacca tgttaatcag
     5641 ttcagcgagc tcaataccat cgacgtcaac gacaatgcgc cccatgttca gtgcctgtac
     5701 gttaacgctg tcggcttcca gtgtcagggc cagtttcatc atttcgcctc cggatactta
     5761 cccagggtaa tgttatttac cgttctgtaa ttgtcgcggg tcatcaggcc ggtcgcccgg
     5821 cgagcccgga ggatatcaat actgtttatt aactgagagc ggggacaggt gctgaatccc
     5881 ggctggtcgg tacgcaccag cgcgtatttt tccacgagaa aattaactgc atcacacagt
     5941 gaaatgcctg cctcaatatg ctgctcaatc acacgttcat cgacaaacgg tgtgtcattc
     6001 agtgtgaggc cataatgctg gtccagcagt cggatgagca gtgtctgcca gatggtgacg
     6061 ggagacgggc agcgagatac ctcccgtaca tgtgtgtcgg ataatgtttt catcttaagt
     6121 tctctgtaac tggtagtgct aaaggtgaga gcttcggggt tatgaggtgg ttgcgggtgc
     6181 tggtgtcgga taaacagcca gataaacgta accacaggag ccgagggtgt cggcttcgca
     6241 ggtcagccct tttgcataca gcgtgacggt atgctgatat cggggattca gttcaccgct
     6301 ggtgagcatg agttccagtt gtttcatcag cagcggaaag gcctggtcca gatggtatgc
     6361 atctgcgtcg ctgaagcggc ctctgatacc ggcgcggtcg gcaaggtaat acaggcggtt
     6421 accctcttgc accagacgag cgccaaaaca gggcctcact gtgcagggaa gcccccacca
     6481 ggggcggtcg tgattgtcat cgggatgagt tatcccggag agtttgtctg acacgataaa
     6541 atcctctctg aaaagtggtt aagaatggtt taacgatgac aggtcagccg gcgcgctgac
     6601 cggtttcggt gaagatgtat gcattgatga gaatggcctc atccacagcg gcgtctgagg
     6661 tcatctggtc gtactcgttt cccgggtgac ggaacatcag ttcagggtga cactcatcag
     6721 ccggacatac gggccaaaac tgtccttacg gcgttctgca aacacggcaa gaacgccggg
     6781 aatatcctgc acttcacgac cagtatacgc ttcagcactg ccgtgccagc gatacttacc
     6841 ggtacagaac ggaaaaagac gggatgcagg atgctgttgg tgaatacgca tggcttcacc
     6901 acgggtgatg attctcatga tgtgatacct ctgaagacag aagataaaag tgaaaacagg
     6961 tgtgatgtgg ttgtgacggg ttaaagcaga ccatgttcgg caaaggagaa aacctggttg
     7021 ccaccgacta tcagatggtc cggcacccgg atatccacca ggcccagtgc ctgtaccaga
     7081 cgttcggtga taagccggtc tgccttactg ggtgtgactt caccggacgg gtgattgtgc
     7141 gccagcacca cggcagcggc attgtggtac agggcgcgtt taatcacttc ccggggatga
     7201 acttccgtac ggttgatggt gccggtgaag agggtttcac cggcaatcag ctgattctgg
     7261 ttgttcagat acagcacccg gaactcttcc cgctccagtc ccgccatgtt cagaatcagc
     7321 cattcccgtg ccgcacgggt ggaggtgaag gccacgccgg gttcatgaag atggcggtcc
     7381 agggtttgca gggcccgcag aatgagactg cgctcgccgg gcgtcatgtt tccgggcaga
     7441 aaggaaagct gctgcattgt ccctgcctcc attcagtcga tgatgcgcat aatggcgctg
     7501 cattccggat gctgcagggc gtaatcccgc agccggtaat aatggaccgt catggcataa
     7561 cactccgtac gacaggcatg atgactgtac gtcatcagac aggcagcaat gccggcggct
     7621 tccgggctca tttcagcgcg gttaccgttc atggcattga acagtaccca tgtttcgtca
     7681 tcatcgtcat ccggttcggg tgccataaat gccccgccgt tgttcagggt gtacagattc
     7741 cagataccac cgcagtagtc ttcacacaga cggtccatcc agccgaagac acggggttcc
     7801 agggtgaccc actgtggaat gaggccaaaa tgctgcggcc agaagctgat gcgctgttca
     7861 tcagggacgg gagtggcaac cagttgttgc aggccagtct ccggtgcaca ggccgaagcg
     7921 gtggtgttct gagacaatgt tttcatgtat gaaatccttg taatgtgaat aaatgagagg
     7981 gagtaaaggt cgggagctgg caacatcacg ttcatcactg accgtgatga cccggtaata
     8041 tcagggatga atgatattcg gctgaaattc cggacagccg tacgcgtaac cgttccggtc
     8101 acagacggat ggccagatgc cattgcctgt atactcagtc tgcacagact gatttctccg
     8161 ggacggtaaa gcagacgggt acaccaggcg cacaggtcat catcagaaat ggaacgacac
     8221 cccggcagcg cctccggggc gtcgggtgat tctggtgcct gaatcacgcc ttcagggaca
     8281 tgctgcatca gcgcaggctc tccagcagcg tttctgccat tacccacaat gcgcggttga
     8341 gcttaatatc ggtgtcgatg ttgtgaatgg cacgggtgtg gatacgtttt ccttttgcac
     8401 tgcggccgga aatcccgcct ttcagcatat tctcctggat ggtctgatat gcgctccaca
     8461 ggtctttacc gtaatcctcc ctgcgtcgtg gtgtcagaat gtcagcggtg gtgactggct
     8521 gatgttcgtc accataacgg taagtcagtg ccgcctgtgc cagcgcctgg cgtgccggtg
     8581 gcggcaggac cagcgactgc atggcatcac gcttttcctc aatccggtca aaaaccccca
     8641 ccacttcgta agccccttcg ataactttct ccaccacatt tccccggtgt ggaacacgca
     8701 cttcccccag agactgaccg cagacgcagc cattctggca gacgaatctg aagtaacccg
     8761 gcagcatctg gtagctggag gtaccgtcat gagagttgag cagaataatt tcagggacat
     8821 gttctccgtt tatctctccg gcccgccgca gacgcagcat gtgttttgtg tatccccggc
     8881 ggcccgggtc gcgcacgcgg gtctggcagg cgaagaatgg ctgaaaacct tcctgctgca
     8941 ggctttccag tacggtgatg gtgggaatgt acgcatatcg tttactgcgg gaagtatgcc
     9001 ggtcttcacc gaaaatactg ggaacatagt gcatcagttc ttcgtgtgtc agcggacggt
     9061 cacggcgtat ctggttcgca taaccaaaac gactggctag tcgcataatt tgctccttat
     9121 cggtggttac gatttactgg tgtaataaat gaaaaagctg cgtctcccgg agaagacgcg
     9181 gcctgacaga tgaaatgaat gacgtttatt gtctgagaag cccttaactg gcgagctgag
     9241 tattaagctg tgttccggca tcaccagcgc aactgacctt cagcattacg gataaccagc
     9301 cgggaatatg ttccctggtc atcttcagta aacacattgc ggtaagctgt tttgacggca
     9361 acagcctgtt cgcgtgagaa agggccttcg ggcaataaac gtgatgtaca acccggcata
     9421 tctgttgctc cctgaaagta aaaagccccg gtcatgatga ccggggcctg aaggagagtg
     9481 acctgattat caaaaagtca cattcagcgt ggcctgaccg ttataacctt cagcgctgct
     9541 gccgctgacg ctgtgggcat aaccggcctg aacgcccagg gtgatatttt cccggacacg
     9601 ggcttccagt ccggcctgca ggtccagtga cgtgccattc cgggacggtg agaacgtcat
     9661 gttactgccg gcggcggctg tccccatgct catgtcaccc cgggagctga aagtgcggat
     9721 aacagaaggc tgtacccacc agttcaccgg cagttcactc acacggtgtt ttgtactgtc
     9781 gcgcagagtg tcacgggatg aggtgccttc accaaagctc atatcgttgt ggctgcccag
     9841 acggaaaccg gcacgcacat gttgtgcact gccatgcccg aacttcacat aaccggcgtt
     9901 atcctggccg tcatccaggg taagtccctg ccaggtatag tgcagttgtg gctccagcat
     9961 caggttgtca gtgatactga agggcagacc ggtttccagt gagcccagcc agccccagcc
    10021 ccgggcacgg aagtcgttat tgtccgatga cgctttcatg ctgtggcggg ttccctgtgc
    10081 cacaatgtca gcccacaggc cggaggacgt gtgtgtcaga ttcaggtatc cgcccaggct
    10141 gccggcatca tcccggaccg tgccggcgcg ggaaccgtca tcatccttaa catcaacgga
    10201 agaatggcct gcggcaccat acacccctgt cgtcagagac ataccggcaa cctctgttct
    10261 cagcaggtca ccctccagac ggacgaagcc atagctgccg ctgctttccg gcgtggctcc
    10321 acgggcaata ccgccgttgt tatcgtgacc gagatgaccg ccctgaatgc tgagacggac
    10381 gctgttattt tcaccgttta caccggtctg atggctgcgg gagcctgcca gaatccggtc
    10441 atagtccatt gcctgtgtca gcatggatgt atacaggggg acttcagcac gataagcatt
    10501 ttcactgcgc aggtaccagt cttcatcgct gtcacggttc agggtgtagt taaaggcgcc
    10561 ggcctgaagc gggcgactca gggcaaacgc accttcttct gtggtggcgc cattctgtgc
    10621 atccacaacc cggataccct gtccggaggt tgccaccccg aggttactgt ttccgacatt
    10681 tgtaaacgca agccaggttt tgccggttgc ctgaccacca ttaatcacca gctggtcaga
    10741 ggtattgctg ccatcaaggc gaacacgcat attgatggtg ccaccctgac cggtgaggtt
    10801 actggtggtc agtttatgga acgttaccgg ggagtcgcct tttgcaacag cacggctcag
    10861 cgcggcatcc ggtgtcgtct gattatcaaa gcgaatctcc cctgcgttaa caatatcgcc
    10921 ggtactgacc acatcgccat tcagtaccat gctggcacct ttactgagct gtgttctgcc
    10981 actcagactt cccccggcag ccagcgtcag tgttccacga tgtccgacag tggtgttgct
    11041 ggccagtcct ccggcattaa ccgtaaagct gccgccattt tccagcagca gtccgctggc
    11101 ctgaccggat gtgccatcaa tactgaactt accgctggca ttggtcccct caacagtggc
    11161 accactgtct gcaatcaggg caccaccgga tgtcatggtg acacctgttg ccttaccacc
    11221 ggcagacact gccagggttc cgccgtcatc cacccgtgtt ttctgcgctg aatggccttc
    11281 cagtacatcc aggcgaccgc cggactccag aacaactccg tcagccttac cgttttccac
    11341 cgtgaaattg cccagacggt tgctgccaag aaccgttgcc gcagtactgg taaccagtgc
    11401 tccaccctgt ttcagggtga catgtgtggc ggtaccaccg gcgttcacct gcagtttgcc
    11461 tttctggtta acggtggtga aatccgccag accaccttcc ttgataatct gccagccgtc
    11521 actgtttaca acagtgccag acgccgtacc gccgttgtgc acatactggt aaccgccatt
    11581 cagtgtggta tccagcgcat gcccgtgtac cgtctggtcg ccgccggcat aaaccacagt
    11641 ggtatttgct gttccttcag cagccacaat ctgacgacca ttttcattga tagtggtacg
    11701 tacggcattt ccgcgaacaa actgcccggt atcaccattt tctgcatctg gtcccccttc
    11761 cgcgccggta ttcacaaccg tgtcggttgc cattgcgccg gatttgacgg tctgccagcc
    11821 cttctcatta atgacggtac ccgttgcaat cccgccttca tgtacccact gctcaccgcc
    11881 gttcagagtg gtattcactg cctgcccctg aaggctctgt ccgcctccgg cactgataac
    11941 cgtgtctgaa acgcttcctc cggcattcac tctctgaaga ccaccaccgg tgacagtagt
    12001 gttgttggcg ataccgccat tttgtatcca ttgcccgccg gtattggcct cgttatccgg
    12061 cccatactcc agaccgctac tgatggtcat tccgttggcc gtaccgagaa caatctggtt
    12121 gtcatgattt gtcagtgttc cgccgttcac ggtttctccc gcctgtacaa tcgtgtcagc
    12181 agccagtgcc gggactgatg tgacagcagc aagagacagc acaaccgcca caccggcgcg
    12241 ttttccccgt gagcgggcca gttcggaagc caccaccagg gtgcccgtaa tgtgattcca
    12301 taccagcctg tagctggtgt tcagatgtcg tttcatcagc ttttccttac agagggtgaa
    12361 taaaaaagcc ggtacccaca aaagtggata ccggcaggta caaaacagca tggtcagata
    12421 aagtggtttt tcccggacat aaatgcttcc cctctctcat ccgccagaga agtatcagtc
    12481 cgttgtgtgc aattccgttc tgcatgatga agagattcag ctgccttttc gcagaccatt
    12541 aaccactata aacgatcgat agcaacgatt gatacctgtt ttatcgatcg ttttattcta
    12601 ttaacgacaa ggcggtcacg caagagaaac aggaggagta tgaggggttt gtgtcatgtt
    12661 ttcagttggt taacgtaatg ttatcaggca tttccctggc agactgttca gaagaaaatc
    12721 cagttccata cagcgcgggc aacagagacc accgtgtcac ggacggcacg cagaaccgta
    12781 cgggcaacag aggcaacaca gacgctctcc gccgtgtcaa atatccggtc caccgcaccg
    12841 gtaaactgtt cacgggcctg accccggaca gattccgtgc gcagctcgtc ctgcaggcgg
    12901 gtcatcaggg gactggcggc atggtcggga agtgctgtca ttagcgcact gaccagcgta
    12961 tccagttccc agccggtgcg ggccgatacg gcaacaaccg gatgtacggg ccggaacaga
    13021 cggaataccg cctccgtttt ttcgcgaata ttctgtgcct gtgcgggaga aggctgaata
    13081 ccggccatat cccattcatg gcagggctcc gttttgtcgg cctgcgtcac cacaaacagc
    13141 acctgctgat gtccccggtg caggatgtgt cgccagaaat actcatccac agacagggca
    13201 cggtcatcgg ctttaatcag ccacagtacc aggtcaagtt caggcagaat gtcacggtac
    13261 agggcttcat actctgcatc tctgtcccgg ctctcgccca ccccgggcag gtcagtgata
    13321 accatgctgt gaccatggcc actcagacgg aaacgctgca cttcccgggt gccggcgtga
    13381 acatcactga ccggggtgac ctccccctga aacagtgcat tacagagtga ggatttaccg
    13441 gccccgcttt tacccataat gccaatcacg ggttcgtgac tggtgagttt gcgcagatgt
    13501 tccaggatgt gacgggaaag tgagtaaggc agggaggaga gcggtttttc aattgcctca
    13561 atggcatcag acggattcat acatgtattc ccggtgaaaa aagacaaaac ccccgcacac
    13621 cggaaaggtg gcgggggact cacggagata atatatgtct gaaattatct ggaatgt
//
LOCUS       DAJTMC010000105        13638 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.105, whole genome shotgun sequence.
ACCESSION   DAJTMC010000105 DAJTMC010000000
VERSION     DAJTMC010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13638
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.105"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(1..1867)
                     /gene="speC"
                     /locus_tag="OO130_002785"
                     /pseudo
     CDS             complement(1..1867)
                     /gene="speC"
                     /locus_tag="OO130_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
     gene            2265..2963
                     /locus_tag="OO130_002786"
     CDS             2265..2963
                     /locus_tag="OO130_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HCR5366123.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRISMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPTHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWFA"
     gene            3069..3144
                     /locus_tag="OO130_002787"
     tRNA            3069..3144
                     /locus_tag="OO130_002787"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3102..3104,aa:Phe,seq:gaa)
     gene            complement(3293..3829)
                     /gene="yghD"
                     /locus_tag="OO130_002788"
     CDS             complement(3293..3829)
                     /gene="yghD"
                     /locus_tag="OO130_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspM family type II secretion system protein
                     YghD"
                     /protein_id="HCR5366124.1"
                     /translation="MLRDKFIHYFQQWRERQLSRGEHWLAQHLAGRSPREKGMLLAAV
                     VFLFSVGYYVLIWQPLSERIVQQETMLQQLVAMNTRLRSAAPDIIAARKSATTTPAQV
                     SRVISDSASAHSVVIKRIAERGENIQVWIDPVVFNDLLNWLNALDEKYALRVTQIDAS
                     AAEKTGMVNVQRLEFGRG"
     gene            complement(3831..5009)
                     /gene="gspL"
                     /locus_tag="OO130_002789"
     CDS             complement(3831..5009)
                     /gene="gspL"
                     /locus_tag="OO130_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HCR5366125.1"
                     /translation="MSSILEIFFPLCAADPIHWQRRTPDVEHGIWSDVANEQLQQWLQ
                     TDAIRLYIPGEWISVWQVELPDVARKQIPTILPALLEEELNQDIDELHFAPLNIDQQL
                     ATVAVIHQQHMRNIAQWLQENGITRATVAPDWMSIPCGVMAGDAQRVICRIDECRGWS
                     AGRALAPVMFRAQLNEQDLPLSLTVVGIAPEKLSAWAGADAERLTVTALPAVTTYGEP
                     EGNLLTGPWQPRVSYRKQWARWRVMILPILLILVALAVERGVTLWSVSEQVAQSRTQA
                     EEQFLTLFPEQKRIVNLRSQVTMALKKYRPQADDTRLLAELSAIASTLKSASLSDIEM
                     RGFTFDQKRQILHLQLRAANFASFDKLRSALATDYVVQQDALQKEGDAVSGGVTLRRK
                     "
     gene            complement(5006..5983)
                     /gene="gspK"
                     /locus_tag="OO130_002790"
     CDS             complement(5006..5983)
                     /gene="gspK"
                     /locus_tag="OO130_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HCR5366126.1"
                     /translation="MITSPPKRGMALVVVLVLLAVMMLVTITLSGRMQQQLGRTRSQQ
                     EYQQALWYSASAESLALSALSLSLKNEKRVHLAQPWASGPRFFPLPQGQIAVTLRDAQ
                     ACFNLNALAQPTTASRPLAVQQLIALISRLDVPAYRAELIAESLWEFIDEDRSVQTRL
                     GREDSEYLARSVPFYAANQPLADISEMRVVQGMDAGLYQKLKPLVCALPMIRQQININ
                     TLDVTQSVILEALFDPWLSPVQARALLQQRPAKGWEDVDQFLAQPLLADVDERTKKQL
                     KTVLSVDSNYFWLRSDITVNEIELTMNSLIVRMGPQHFSVLWHQTGESE"
     gene            complement(5986..6585)
                     /gene="gspJ"
                     /locus_tag="OO130_002791"
     CDS             complement(5986..6585)
                     /gene="gspJ"
                     /locus_tag="OO130_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HCR5366127.1"
                     /translation="MRRTRAGFTLLEMLVAIAIFASLALMAQQVTNGVTRVNSAVADH
                     DQKLNLMQQTMSFLTHDLTQMMPRPVRGDQGQREPALLAGAGVLASESEGMRFVRGGV
                     VNPLMRLPRSNLLTVGYRIHDGYLERLAWPLTDAAGSVKPTMQKLIPADSLRLQFYDG
                     TRWQESWSSVQAIPVAVRMTLHSPQWGEIERIWLLRGPQ"
     gene            complement(6582..6953)
                     /gene="gspI"
                     /locus_tag="OO130_002792"
     CDS             complement(6582..6953)
                     /gene="gspI"
                     /locus_tag="OO130_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HCR5366128.1"
                     /translation="MKRGFTLLEVMLVLAIFALAATAVLQIASGALSNQHVLEEKTVA
                     GWVAENQTALLYLMTREQRAVRHQGESDMAGSRWYWRTIPLNTGNALLQAVDIEVSRH
                     EDFSSVIQSRRAWFSAVGGQQ"
     gene            complement(7013..7513)
                     /gene="gspH"
                     /locus_tag="OO130_002793"
     CDS             complement(7013..7513)
                     /gene="gspH"
                     /locus_tag="OO130_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001115132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HCR5366129.1"
                     /translation="MPERGFTLLEIMLVIFLIGLASAGVVQTFATDSEPPAKKAAQDF
                     LTRFAQFKDRAVIEGQTLGVLIDPPGYQFMQRRQGQWLPVSATRLSAQVTVPKQVQML
                     LQPGSDIWQKEYALELQRRRLTLHDIELELQKEAKKKTPQIRFSPFEPATPFTLRFYS
                     AAQNAC"
     gene            complement(7517..7972)
                     /gene="gspG"
                     /locus_tag="OO130_002794"
     CDS             complement(7517..7972)
                     /gene="gspG"
                     /locus_tag="OO130_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003912123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HCR5366130.1"
                     /translation="MNSLSRTHKPRAGFTLLEVMVVIVILGVLARTVVPNLLGNKEKA
                     NRQKAISDIVALENALDMYRLDNGRYPTTEQGLEALIQQPANMADSRNYRTGGYIKRL
                     PKDPWGNDYQYLSPGEKGLFDVYTLGADGQENGEGAGADIGNWNLQEFQ"
     gene            complement(7989..9212)
                     /gene="gspF"
                     /locus_tag="OO130_002795"
     CDS             complement(7989..9212)
                     /gene="gspF"
                     /locus_tag="OO130_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HCR5366131.1"
                     /translation="MALFYYQALERNGRKTKGMIEADSARHARQLLRGKELIPVHIEA
                     RMNASAGGMLQRRRHAHRRVAAADLALFTRQLATLVQAAMPLETCLQAVSEQSEKLHV
                     KSLGMALRSRIQEGYTLSDSLREHPRVFDSLFCSMVAAGEKSGHLDVVLNRLADYTEQ
                     RQRLKSRLLQAMLYPLVMLVVATGVVTILLTAVVPKIIEQFDHLGHALPSSTRALIAM
                     SDALQASGVYWLAGLLALLVLGQWLLKNPTMRLRWDKTLLRLPVTGRVARGLNTARFS
                     RTLSILTASSVPLLEGIQTAAAVSANRYVEQQLLLAADRVREGSSLRAALAELRLFPP
                     MMLYIIASGEQRGELETMLEQAAVNQEREFDTQVGLALGLFEPALVVMMAGVVLFIVI
                     AILEPMLQLNNMVGM"
     gene            complement(9212..10704)
                     /gene="gspE"
                     /locus_tag="OO130_002796"
                     /pseudo
     CDS             complement(9212..10704)
                     /gene="gspE"
                     /locus_tag="OO130_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525635.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
     gene            complement(10704..12764)
                     /gene="gspD"
                     /locus_tag="OO130_002797"
                     /pseudo
     CDS             complement(10704..12764)
                     /gene="gspD"
                     /locus_tag="OO130_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234996.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
     gene            complement(12794..13623)
                     /gene="gspC"
                     /locus_tag="OO130_002798"
                     /pseudo
     CDS             complement(12794..13623)
                     /gene="gspC"
                     /locus_tag="OO130_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238556.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
BASE COUNT         3054 a         3931 c         3518 g         3135 t
ORIGIN      
        1 agcgctatgc gcatcctggg ggttcatcac cactgacggg aaactctgct ggcggaacat
       61 cgctttttgt aggtctttga cgtcgaaact gacatacaga tcgtgcatct cctgacacaa
      121 ctggcgcaaa gtgtagtcgc gatagcgcac cggatatttg ttataaacgc tcggcaacac
      181 ctcagccagc ggcgagtcat cctcaatatg ctgttcaaac tgcgccaaca tctctaccag
      241 ttgtgccagc ttctcgtggc tttccgccgg agttaataag aacagaatgg agttgagatc
      301 gcacttctcc ggcacaatgc cgttctcacg cagatagtgc gccagaatcg tcgccggaac
      361 gccaaagtcg ctatattcgc cggtttcggc attgatacct ggtgtagtga gtaacagctt
      421 gcacggatca acaaaatact gatccgcggc atatccttca aagccgtgcc acttcgcccc
      481 cggctcaaaa ctgaaaaaac ggcggtcgct ggctaacact gatgtcggat aatcctgcca
      541 caatttgcca tcaacaacgg gcgggataaa cgggcggaac agcttacagc gcgcaagaat
      601 agccttgcgc gactcaatac ccaactcaac gcactcagcc cacagccgac gcccactctc
      661 cccttcatga attttggcgt taacatccag tgcagcaaac agcggataga aagggctggt
      721 agaagcatgg agcataaagg cgttattcaa ccgcttatgc gggcaaaaac gcgcctgtcc
      781 gcggatatgg ttatcttttt tatggatctg cgacgtctgt gagaatcccg cctgctgttt
      841 gtgcaccgac tgagtcacaa agatctccgg atcgttttcg ttaagttcta atagcagcgg
      901 cgagctatcc gccatcatcg ggataaactg ttcgtaaccg acccacgcgg aatcaaacag
      961 aatgtaatca cacagatgcc caacggtatc gatcacctga cgggcgttat agacagttcc
     1021 gtcataggtt cccagctgaa taatcgccag gcgaaacggg cgcggcagct cggctttttc
     1081 tggcgcaacg tcgcgaattt gctggcgcag atactcttca ttaaaacagt gcgcatcaat
     1141 accgccaatg aaaccaaacg ggttgcgtga agcttccaga tagaccggcg tcgcccccgc
     1201 ctgaatcagc gcgccgtgat gattcgactt atggttgtta cggtcgaaga gcaccagatc
     1261 gccacgcgtt aacagcgcat tcgtcaccac tttattcgct gccgatgtgc cgttcagcac
     1321 aaaataggtt ttatcggcat gaaagacttt ggctgcgaat ttctgcgcat ctttcgccaa
     1381 taagcagatc gcccaatttt acgtcagcgt tacacatatc ggcgcgaaag acgttctcac
     1441 caaagaaatc gtaaaaatgg cgtccggcag gatgcttttt aaaaaacgca ccatgttgat
     1501 gtccagggca agcaaaggtg ctgttgccca tctcaacgta ctgcgtcagc gtgtcataaa
     1561 acggtggcag caaattctct tcatactgac aggctgcgga ttccagctcc agccactgct
     1621 gctcgttgcc gttgattacc gccgtaacgc ccgcaggtaa ttcaacagca tgttcggaat
     1681 acaaaaacac cggtagatga aaaccggtgc gcttaagcaa cgcaagaatg ccactgcgac
     1741 tatccgcagc ggtaatgacg actgccacga cgtccgtaaa atcagtatct cccaacgcca
     1801 ccacgcgacg atgagaagaa agtcgggata ccagttcact actggcggca atattcattg
     1861 atttcataag cgcaaacccg tttcggggaa gtaagaatac cggacaaggt ggaaaaccct
     1921 gccccatgag atatgggtca aactggtcac cagctccgac cgccagacat cagtaaaagc
     1981 agaaacgctc tgattttact gttgtcctgc agtgagcgtg cgttaacttc accgcatgag
     2041 cagtaacata gaaagggaaa cgtttcgcgc gaaacggcga taagcgagag aatgtaagga
     2101 ggtggcgtgc atcgggcaaa ctccgtacag agaggagaaa attcgcgcaa tcatggcacc
     2161 tttcgctaaa gcgtgcaagc caggactttg cgaccagaca agccatcaac aaggcaaaaa
     2221 gttcataata agaaaatcaa acaatacagt taacaggaac tcttgtggtc atcggccctt
     2281 ttatcaacgc tagtgcagtc ttactgggtg gcgttctcgg cgcactgctc agccaacgct
     2341 taccggaacg tatccgcatc tccatgacat ctatttttgg tctggcatcg ctggggattg
     2401 gtattttact ggttgtgaaa tgcgccaacc ttccagcgat ggttttagct acgctacttg
     2461 gcgctctaat tggcgaaatt tgtttgctgg agaaaggtgt caatacagca gtcgccaaag
     2521 cacaaaatct gtttcgtcac tcacgtaaga agccaacgca tgaatctttt attcagaatt
     2581 atgtcgcgat tattgtcctg ttttgcgcca gcggcaccgg gatcttcggg gcgatgaacg
     2641 aagggatgac cggcgatccg agtattttaa tcgccaagtc atttcttgat ttctttacgg
     2701 cgatgatctt cgcctgctcg ctgggtattg cggtgtcggt aattagtatc ccattactga
     2761 tcatccagtt aacgctggcg tgggctgccg cgctgatatt accgctgacc acaccgtcga
     2821 tgatggcaga cttcagcgcc gtaggcggtt tattgctgct ggcaaccgga ttacgcatct
     2881 gtggcattaa aatgttcccg gtggtcaaca tgcttccagc actccttctg gcaatgccgc
     2941 tttccgccgc ctggtttgcc tgacaatgcg tgcaatagcg gcaaagtgat gatagattgt
     3001 gcagtctgca gtaaattgaa gaaatttgat tgacgagacg aggcgaatca ggtttaatgc
     3061 gccccgttgc ccggatagct cagtcggtag agcaggggat tgaaaatccc cgtgtccttg
     3121 gttcgattcc gagtccgggc accactaatt cttaagaacc cgcccacaag gcgggttttt
     3181 gcttttggat ctgacaataa ccttcacgaa aaaagtttgc ttataaagtc tgggggaatt
     3241 actctcgcca cgttaacgag agtaatttta ttgatactaa tctcctgata ctttaccccc
     3301 gtccaaactc cagccgctgg acattcacca tcccagtctt ctcagcagca ctggcatcaa
     3361 tttgtgtcac ccgcagcgca tacttttcat ccagcgcgtt taaccagttc agcaggtcat
     3421 taaacaccac cggatctatc cagacctgaa tattctcccc acgctcggct atccgcttga
     3481 tgactaccga gtgcgcggaa gcactgtcac tgataacccg cgatacctgc gcaggcgttg
     3541 ttgtggcgga ttttcgcgcc gcaataatat ccggcgcggc gctcctcagt ctggtgttca
     3601 tcgccaccag ttgctgcaac attgtctcct gttgtactat ccgttcgctc aacggctgcc
     3661 agatgaggac gtaatatccg acgctaaaca ggaacaccac cgctgccagt aacatgccct
     3721 tttcacgcgg agaacgcccc gccaggtgtt gtgccagcca gtgttcgcca cggcttaact
     3781 ggcgttcacg ccattgctga aaatagtgaa taaatttatc gcgtaacatg ttatttcctc
     3841 cgcaacgtta cgccgccgga aaccgcatca ccctctttct gtaacgcgtc ctgttgcaca
     3901 acataatcgg ttgccagtgc actacgcagt ttgtcgaaac tggcaaagtt cgcggcccgt
     3961 agctggaggt gaagtatctg gcgtttttga tcaaaggtaa aaccacgcat ttcgatgtcg
     4021 gaaagtgacg ctgatttcag ggtgctggct atcgctgaca actcggcgag cagccgggta
     4081 tcgtcggcct gtgggcgata ttttttcagc gccatcgtca cctgagagcg taaattcaca
     4141 atccgcttct gctccgggaa caacgttaag aactgttcct ccgcctgggt gcggctttgc
     4201 gccacctgtt cgctgacgct ccataacgtc acgccccgct ccactgccag cgcaactaga
     4261 atcagcaata tcggcagaat catcacccgc cagcgcgccc actgttttcg gtagctgacg
     4321 cgaggctgcc acggccctgt cagcaggttc ccttccggtt cgccataagt ggtaacggcg
     4381 ggcagagctg taacggtcag gcgttcagcg tctgcgccag cccatgctga tagcttttcc
     4441 ggtgcaatgc cgactacggt tagcgaaagc ggtaaatcct gctcattgag ctgggcgcgg
     4501 aacatgaccg gagccagcgc ccgcccggcg ctccatcccc ggcattcatc aatgcggcag
     4561 ataacccgtt gcgcatcgcc agccataacc ccacaaggaa tggacatcca gtctggcgcg
     4621 acggtagcgc gggtgatgcc gttttcctgc aaccactgcg caatgttgcg catatgctgt
     4681 tggtgaatca ctgctacggt tgccagttgc tggtcgatat tcaacggggc gaaatgcagt
     4741 tcatcgatat cctggttcag ctcttcttcc agtaaggcgg gcagaatggt cggtatctgc
     4801 ttacgggcga catcaggcag ttcaacctgc cagacgctga tccattcgcc gggaatgtag
     4861 agtcgaatcg catcggtttg cagccattgc tggagctgtt cgttagcaac gtcagaccag
     4921 ataccgtgct ccacatcggg tgtacggcgc tgccaatgga tgggatcagc ggcgcaaagc
     4981 gggaaaaaaa tctcaaggat ggaactcact cactttctcc tgtctgatgc cagagaaccg
     5041 aaaagtgttg tgggcccatg cggacaatta acgagttcat cgtcagttca atctcattca
     5101 cggtgatatc tgaacgcagc cagaagtaat tgctgtccac gctcaggacg gtttttagct
     5161 gttttttagt acgctcatcg acgtcagcaa gtagcggctg tgcaagaaac tgatcgacat
     5221 cttcccagcc cttcgccgga cgttgttgta ataacgcccg cgcctgaacg gggcttaacc
     5281 acgggtcaaa cagcgcctca agaatcacac tttgcgtgac gtctaaagta ttgatgttga
     5341 tttgctggcg gatcatcggc agcgcacaga ccagcggttt cagtttttga taaagcccgg
     5401 cgtccattcc ctgtaccacg cgcatctcac tgatatcagc cagcggttga ttggcggcgt
     5461 agaacggcac cgaacgggcg agatactcgc tgtcttcacg gcccagacgt gtctgcacgc
     5521 tgcggtcctc gtcaataaac tcccacaggc tttcggctat cagttcggcc cgataagcag
     5581 gcacatccag gcgcgagatc agggcaatca actgttgtac cgcaagcgga cgcgacgccg
     5641 ttgtcggctg agcaagggca ttcaggttaa agcaagcctg tgcgtcacgc agggtgaccg
     5701 caatttgccc ctgcggcaga gggaagaaac gagggccgga tgcccacggc tgtgccagat
     5761 gcacgcgctt ttcatttttc aggctcagac tgagcgcgct cagcgccagg ctttccgcac
     5821 tggcgctgta ccacagcgcc tgctggtact cctgctggct gcgcgttcgc ccaagttgtt
     5881 gctgcattcg cccggaaagc gtgatggtta ccagcatcat aaccgccagc aataccagca
     5941 ccacgaccag tgccattccg cgttttggtg gtgaggtgat catgattatt gcggcccgcg
     6001 taacaaccag atgcgttcaa tctcgcccca ttgcggcgaa tgcagggtca tgcgtaccgc
     6061 cacagggatc gcctgtactg acgaccagct ctcctgccag cgcgtgccgt cgtagaactg
     6121 caaacggagc gaatcggccg gaatcaactt ttgcattgtc ggcttcacgc tgcctgcggc
     6181 atcggtcagc ggccaggcta accgttcgag ataaccgtca tgaatgcggt aaccgacggt
     6241 gagcagattg ctgcgcggca gacgcatcaa cgggttaacc acaccgccgc gcacaaaacg
     6301 cattccttca ctctcagacg ccagcacgcc agcgccagcc agtaaagctg gttcacgctg
     6361 gccctgatcg cctcttaccg gacgcggcat catttgggtc agatcgtggg tcagaaaact
     6421 catcgtttgc tgcatgaggt ttagtttttg atcgtgatcg gcgacggcgc tattcacgcg
     6481 cgtgacgccg ttagtcacct gctgcgccat cagtgccagc gaggcaaaaa tggcaattgc
     6541 caccaacatt tccagtaacg tgaaaccagc gcgagtcctt ctcactgttg gcctcccacg
     6601 gcgctaaacc aggcgcgtcg tgactgaatc accgacgaaa agtcttcgtg acggctgact
     6661 tcaatatcca ccgcctggag cagcgcgttg cctgtattca atgggattgt tcgccagtac
     6721 cagcggcttc ctgccatatc gctctcgccc tgatgcctga ccgcccgctg ctcacgggtc
     6781 atcaggtaga gcagcgcagt ctggttttca gctacccagc ccgctaccgt tttttcctca
     6841 agaacgtgct gattactcag cgcaccgctg gcaatctgta acaccgccgt ggcagccagc
     6901 gcaaaaatcg ccagcacgag catcacttcc agcaaggtaa atccgcgctt catggcatcc
     6961 tctcatcaca ttgattgagg gataacgcgc catcgtgtgc cagctttacc gctcaacatg
     7021 cgttttgcgc cgccgagtag aagcgcagcg taaacggcgt ggcgggttca aaaggcgaaa
     7081 aacggatctg cggcgtcttc tttttcgcct ctttttgcaa ctccagttca atatcgtgca
     7141 gcgtcaggcg acgacgttgc agctccagcg catattcctt ctgccagata tcacttccgg
     7201 gttgtaacag catctgcacc tgtttcggca ccgtaacctg tgccgataac cgggtcgcag
     7261 aaacgggcaa ccactgcccc tgacgacgct gcataaactg atagccaggc gggtcgatta
     7321 gcacaccgag tgtctgtcct tcaatcactg ccctgtcctt aaactgcgca aagcgagtca
     7381 gaaaatcctg tgccgctttt ttcgcgggcg gttcagaatc ggtcgcaaac gtctgtacca
     7441 cgcccgcact ggcaaggccg ataaggaaaa tcaccagcat gatttccaga agtgtgaatc
     7501 cgcgttcagg cactgcttac tgaaactcct gcaaattcca gttaccgata tctgcgccag
     7561 cgccttcccc gttttcctga ccatccgccc ccagggtata aacatcaaac agtccttttt
     7621 cacccgggct gagatactga taatcattgc cccacggatc ctttggcagt cgtttgatgt
     7681 atccaccagt acggtagttg cgggagtccg ccatattggc tggctgctgg atcagcgctt
     7741 ccagcccctg ttctgtggtc ggataacgcc cgttatccag tcggtacata tctagtgcgt
     7801 tttccagcgc cacgatatcg ctgatggctt tttgccgatt ggctttctct ttgttgccca
     7861 acaggttagg caccaccgtc cttgccagga cgccaagaat aacaatcacc accatcactt
     7921 ccagcagggt aaaacctgcc cgtggtttgt gtgtgcggga taacgaattc atgtgataac
     7981 tccgtaaatt acattccaac catattattc agttgcagca tcggctcgag gatggcgatg
     8041 acaataaaca gcaccacgcc cgccatcatc accaccagcg caggctcaaa cagccccaac
     8101 gccagcccca cctgggtatc aaattcccgt tcctggttaa cagcggcctg ctcaagcatg
     8161 gtttccagtt cgcctctctg ttcgccggaa gcgataatgt acagcatcat cggcgggaac
     8221 aggcgcaact ccgccagcgc ggcgcgcaga ctgcttcctt cgcggacgcg atctgccgcc
     8281 agcagcagtt gttgttcgac atagcgattt gccgacacgg cggcagcggt ctgaatgcct
     8341 tccagtagcg gaacactgct ggcggtgagg atgcttaatg tgcgggaaaa acgcgccgta
     8401 ttcagcccgc gcgcaacacg gcccgtcacg ggcagacgca gcaaggtttt atcccagcgc
     8461 aggcgcatag taggattttt aagtagccat tgccccagca ccagaagcgc cagcaatccc
     8521 gccagccagt aaacgccgct ggcctgtaac gcatcactca tagcgataag cgcacgggtg
     8581 gaggagggta gcgcgtgtcc gagatgatca aactgttcga tgattttcgg caccactgcc
     8641 gtcagcaaaa tagtgaccac gcccgttgcc accaccagca taaccagcgg atagagcatg
     8701 gcctgcaata gacgtgattt caggcgctgt cgctgttcgg tgtaatctgc caggcgattg
     8761 agcaccacgt cgagatgccc ggatttttct ccggcagcga ccatcgaaca aaacagggaa
     8821 tcaaagacgc ggggatgttc gcgcaggctg tccgacaggg tataaccttc ctgaatccgg
     8881 ctgcgcagcg ccattccgag gctcttcaca tgcagttttt cactttgctc actgaccgcc
     8941 tgcaagcagg tttccaacgg cattgctgcc tgcaccagcg ttgccagttg gcgcgtgaac
     9001 agcgccagat ctgccgccgc cacgcgacga tgtgcgtgcc gccgacgctg caacattccc
     9061 cctgccgatg cattcatcct ggcttcaatg tgcacgggga taagctcttt accgcgcaac
     9121 aactggcggg catgacgcgc ggaatccgcc tcaatcatgc ctttggtttt gcgaccatta
     9181 cgctccagcg cctgatagta aaacagtgcc attacgcctc catggttacc cgcagaactt
     9241 catcgagaga ggtttctccg gcgagcactt tctcaatgcc gttgctgcgg atacccgcag
     9301 agtgttgccg gacataacgt tccagctcca gctccccggc ctgacggtgg atcaaatcac
     9361 gcaatgtggc atccaccacg tcagctcatg gatggcagtc cgtccgcgga aacctttgtg
     9421 attacaggcg gggcagccct gcggatggta cagagtgacg gttcgcgcat cggtaatgcc
     9481 cagcaggcgt ttttcttcgt cagtggcagg cgcggcctga cggcagtcgg gacacagcgt
     9541 gcggaccagt cgctgcgcca tcacgcccgt cagactggaa gagagcagga aaggctccac
     9601 gcccatatcc tgcaaacgcg tgatcgcccc caccgccgtg ttggtatgca gcgtggaaag
     9661 gaccaggtgt ccggtaagcg atgcctgaac agcgatttct gcggtttcgg tatcgcggat
     9721 ttcaccgacc atcaccacat ccgggtcctg acgcaaaatc gcgcgcaggc cacgggcgaa
     9781 ggtcatgccg acgcgggtgt taacctgtgt ctgaccgatc ccttcaatca tgtattcgat
     9841 ggggtcttca accgtgagaa tgttacgcga atggttgttc agctcctgca atccggcgta
     9901 cagcgtggtg cttttgccgg aacccgtcgg ccccgtcacc agaaagatgc cgtgcggttt
     9961 gtgtaacagc tggcgcaact gcacggtcag ttgctggcta agccccagac gctccagcgt
    10021 caggcgggcc tggtttttgt ccagcagtcg cagcaccacg cgctcgcccc aggcagaagg
    10081 catggtggag acacgcacgt caatcgcccg gccgcccagc agcagcgcaa tacgtccatc
    10141 ctgcggtacg cgcttttcgg cgatatccag ccgtgccatc accttgatac gcgataccag
    10201 cagcgaggcc agtttgcgcc ccgggcgcag catttcatgt aatgtgccgt caacgcgaaa
    10261 acggatcacc agactctttt caaacgtctc gatgtggata tccgaagcgc cttctttaat
    10321 cgcctctgcc agcatggcgt tgatcagttt gatgatcggc gcatcgtcgt cactttccag
    10381 cagatcttcc gtttctggca gttcttccgc gagagtaaaa aagtcctcag cagaaccgag
    10441 atcttccatc agctggcgag cctcggaaga gtcccgctgc cagaccgcat tcagccgctg
    10501 ttcaaattcg gcctcgtcga tttgccgcag cgtaaagggc gcgttcagcc cccgctgcag
    10561 ctcctgcaaa acagagagcg atagcggatg aacgtggagg atctccagcg acgcttcgca
    10621 ccatgccacc aggctaaacc gacggctgaa actgtagggc agacgcacgg tcttggcggt
    10681 ggtttcctgt gctacaggca ccattaacgc gttctcccgg cattgaggaa cgcgcgaact
    10741 tccggcggta aggcctggtt ttgcgctggc agtaccggct gtgcggtgtg cggcatcagg
    10801 cttaaacctt gctcatcacg atagatctgt tcggcgcgca tatagttata tttgcgctgc
    10861 gacacgccgt ctgccgccat accgtcatgc agaatggtcg ggcggataaa caccatcagg
    10921 ttacgttttt cttttttatc cgccgtcgat ttaaacaggt taccaatcaa tgggatatcg
    10981 cccagcagag gcactttcgc cacgctttct cccgcctgat cgtccatcag accaccaagc
    11041 acaatcagct caccatcgtt agccagcacg gtggttttca gtttgcgctc accaaacacc
    11101 acgtcgaggc tggtctgtcc ttccaccttc gagacttcct gctcaatcac catctgtacc
    11161 gcgttacctt cgttaatctg cggcgtgact ttcagcatga tgccgacttt tttcctttct
    11221 actgtattga aaggattgct gttattggag ccaacggtag agccggttaa taccggaacg
    11281 tcctagccca ccatgaagaa ggcttcctgg ttgtccagcg tagtgatgct cggcgtggag
    11341 agaacgttcg agctggagtc gtttttaacc gcctgcacca gtgccatcca gtcgcctttc
    11401 accacgccaa ccgccgtacc gctaaatcca gaaagaagct gggcgagtgt ggagagatcg
    11461 ccgttggtat ccggattaat ggtggtagcg ccgttttcac tgatcaccgt cgagcctttc
    11521 tgcggtttcg cctgagaaat ggctgccccc agcgtaccga tagggatctg cgtaccatta
    11581 gcaaactgca ttaatccggc atctttcgat gcccactgca cgccgaagtt gatattgcta
    11641 ccttcggcaa cttccacgat caacgcctcg acgtgcacct gagcacggcg aatatccagt
    11701 tgttcaatta cgctttgcag cgactgcatg atgtcctgcg gcgcggttac aatcagggca
    11761 ttactgtgtt tgctggcggc gatggataca acctcacgcc cgctaccgac cgtgccttcc
    11821 gcctcttctt tagccgccgt gagcgtgccg ctgacctgtt tcagcacatc gaccagatct
    11881 tcggctttgc tgtatttgag atagaaaacc tggctgttgc cgctgcgttc catttctgag
    11941 tccagccgac ggatcaggcg gcgcattttg tcccgcgtgg ccgggtcacc actgacaatc
    12001 acactgttgg tgcgttcgtc ggcgacaatt tgagatttca gcgtcgcagg ctggttctcg
    12061 ccgctgtttt tcgtcaggct ttccagcacg cgggcgattt ccgaagccga ggcgttatcc
    12121 agcgggatca cctcttcggt gcgattaccc gcgtgatcca cgcgctggat cacttccgtc
    12181 aggcgctcca caacggaggc gcgtccggtg agcataatca cgttggaggg atcgtaatta
    12241 acaacgttgc ctgagcctgc gctgtcgatc atctggcgca gaatcggtgc cagctcgcgt
    12301 accgacacat tacgtaccgg cactactttg gtgaccattt catcgcccgc gtagttgtcg
    12361 ctgccttcac cgaccagcgg cagcggctcg actttcgcag cgctggattt caccaccttc
    12421 agcacgtcgt tttccatcgg tacgacggca tacccctgtg cttccagcag gttaaggaat
    12481 agctggtaat actggcgttc attgagcggg gtcatggtgc gaatactcac tttcccctgt
    12541 acgcccggcc ccataatgat ggttttatta aggttagcgc cgacggtttc gatgaacgat
    12601 ttcaggtcgg tatctttaaa attagcggtg aaagtggctt cttccgccca gaccggtgaa
    12661 ctgcataatg ccgctgccag caccagcggc agtaaacgct tttttgtttt gaggccagtt
    12721 gtcttcttac gccagaccga caaggtaata tcacgccaaa acacgatgaa tgattctcct
    12781 ggattaaatg cggttagcgc agcgcgatgg aaatgtcgtg gcgcgcaccc ttgcgtaaaa
    12841 ccgtaagttg aatggaatcc attgaaggta actgccgcat cagagcaatc attgctcgtg
    12901 gatcagtgaa atcctgctga tttagcgcaa tggcgatatc gccttccttg aaaccgctgg
    12961 catcgaacag agaacgatct gcccccggct tcactgcata accgacaatt ccctccttac
    13021 gcacaggcgt aagctggata tagttaaaaa ttttctgcgg atctttcgcc agtgcctgac
    13081 gcacggcagt cggtatctca actggcgcac tgacagcagg ttcagcaaca gcttgctttg
    13141 cttcgtcact gacagctttt ttgttggtcg cggcaacggt ggaacgctcc tcttcagcca
    13201 ggctcaggcg ctctattttt ccctgatagc gcagcatcac atggtcgcga ttgatttcct
    13261 caatcaccgc gttgtgcgag ccaagcgttt caccctgcaa atagacctgc tgtttaccgc
    13321 cttcttcaat aaccgcgccg ggtctggcac caaaggcgat cccgcgcgca ccacattaag
    13381 acgcgtttct gccacaggca caggttcggg ttgttttacc ggcgtggcga cgggctgata
    13441 tttgccaaac cagttttgct ggctgattaa ttgcacgtca tttttatcga acgtttttgc
    13501 atccgcacgg ggcggtttat tcgccgatgg ggaaacagcc gtatattccg cagaaaagga
    13561 gaaatagcgc cagagtgaat gcgccacttt tgcagaaata ataagcagca tcagccagaa
    13621 catcccgcgt acaatttt
//
LOCUS       DAJTMC010000106        13591 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.106, whole genome shotgun sequence.
ACCESSION   DAJTMC010000106 DAJTMC010000000
VERSION     DAJTMC010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13591
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.106"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            307..642
                     /locus_tag="OO130_002799"
     CDS             307..642
                     /locus_tag="OO130_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HCR5366132.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDTEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            802..1245
                     /gene="yjdN"
                     /locus_tag="OO130_002800"
     CDS             802..1245
                     /gene="yjdN"
                     /locus_tag="OO130_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HCR5366133.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            1378..2166
                     /gene="phnC"
                     /locus_tag="OO130_002801"
     CDS             1378..2166
                     /gene="phnC"
                     /locus_tag="OO130_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HCR5366134.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHSEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRAHTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTREQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQQFDNERFDHLYRSINRIEENAKAA"
     gene            2191..3207
                     /gene="phnD"
                     /locus_tag="OO130_002802"
                     /pseudo
     CDS             2191..3207
                     /gene="phnD"
                     /locus_tag="OO130_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
     gene            3333..4112
                     /gene="phnE"
                     /locus_tag="OO130_002803"
     CDS             3333..4112
                     /gene="phnE"
                     /locus_tag="OO130_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HCR5366135.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFVADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            4133..4858
                     /gene="phnF"
                     /locus_tag="OO130_002804"
     CDS             4133..4858
                     /gene="phnF"
                     /locus_tag="OO130_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HCR5366136.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGIALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            4859..5311
                     /gene="phnG"
                     /locus_tag="OO130_002805"
     CDS             4859..5311
                     /gene="phnG"
                     /locus_tag="OO130_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HCR5366137.1"
                     /translation="MHADIATRQHWMSMLAHSQPAELAARLNALNITANYEVIRTAET
                     GLVQIQPRMGGTGERFFAGDATLTRAAVRLTDGPLGYSWVLGRDKQHAERCALIDALM
                     QQSRHFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            5308..5892
                     /gene="phnH"
                     /locus_tag="OO130_002806"
     CDS             5308..5892
                     /gene="phnH"
                     /locus_tag="OO130_002806"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HCR5366138.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLSAPLSNDIVSQSLRFHTNAPLVNQSELATFAVTDEAIS
                     SEQLNALSSGTAVAPEAGATLILQVVSLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            5892..6950
                     /gene="phnI"
                     /locus_tag="OO130_002807"
     CDS             5892..6950
                     /gene="phnI"
                     /locus_tag="OO130_002807"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HCR5366139.1"
                     /translation="MYVAVKGGEKEIDAAHALQESRRRGNTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQSPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIMPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHYVD
                     FQAELELLKRLQQEQNHG"
     gene            6943..7788
                     /gene="phnJ"
                     /locus_tag="OO130_002808"
     CDS             6943..7788
                     /gene="phnJ"
                     /locus_tag="OO130_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HCR5366140.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     LIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKNQ"
     gene            7785..8543
                     /gene="phnK"
                     /locus_tag="OO130_002809"
     CDS             7785..8543
                     /gene="phnK"
                     /locus_tag="OO130_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HCR5366141.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIHYESRSLYAMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            8654..9334
                     /gene="phnL"
                     /locus_tag="OO130_002810"
     CDS             8654..9334
                     /gene="phnL"
                     /locus_tag="OO130_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HCR5366142.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLQRASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIHEAKARGAAIVGIFHDEAVRNDVADR
                     LHPMGASS"
     gene            9331..10467
                     /gene="phnM"
                     /locus_tag="OO130_002811"
     CDS             9331..10467
                     /gene="phnM"
                     /locus_tag="OO130_002811"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HCR5366143.1"
                     /translation="MIINNVKLVLENEVVHGSLEVQDGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLNAVAIGDVR
                     DGGDQLENLEKMINTIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAL
                     CRARQIALASHDDATHAHVAESHQLGSVIAEFTTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDESNRFTLPQAVRLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRQGNHIHIDHVWRQGKRVF"
     gene            10467..11024
                     /gene="phnN"
                     /locus_tag="OO130_002812"
     CDS             10467..11024
                     /gene="phnN"
                     /locus_tag="OO130_002812"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HCR5366144.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAELRLREQTQLLVAHRYITRDASA
                     GSENHIALSEREFFTRAGQNLLALSWHANGLYYGVGIEIDLWLHAGFDVVVNGSRAHL
                     PQARARYQSALLPVCLQVSPEILRQRLKNRGRENTSEINARLARAARYTPQDCHTLNN
                     DGSLRQSVDTLLTLIHQKEKHHACL"
     gene            11011..11460
                     /gene="phnO"
                     /locus_tag="OO130_002813"
     CDS             11011..11460
                     /gene="phnO"
                     /locus_tag="OO130_002813"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HCR5366145.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFNANLRD
                     PSMRYPSMRYHLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQACGLNVSSKL
                     LAWAEEEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSHFRFTKAL"
     gene            11462..12220
                     /gene="phnP"
                     /locus_tag="OO130_002814"
     CDS             11462..12220
                     /gene="phnP"
                     /locus_tag="OO130_002814"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HCR5366146.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCECVACARARRSPQYRRQPCSGVV
                     KFNDAITLIDAGLHDLADRWPPGSFQQFLLTHYHMDHVQGLFPLRWGVGDPIPVYGPP
                     DEQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLRNNQPQVMVIDCSHPPREDAPRSHCDLNTVLALNQVIRSPRV
                     ILTHISHQFDAWLMENALPSGFEAGFDGMEIGVA"
     gene            complement(12367..12696)
                     /gene="yjdP"
                     /locus_tag="OO130_002815"
     CDS             complement(12367..12696)
                     /gene="yjdP"
                     /locus_tag="OO130_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjdP"
                     /protein_id="HCR5366147.1"
                     /translation="MKRFPLFLLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNPDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(12765..13082)
                     /locus_tag="OO130_002816"
     CDS             complement(12765..13082)
                     /locus_tag="OO130_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HCR5366148.1"
                     /translation="MQPVFLFKKRTGAMGGHVAGTAGFCVAVPAFYSWSMSEVIDKDT
                     RSSERTDYPHYASEVALAFGSRVVGLELAKMIVDAWLGAQYEGGRHQQRVEAITAIEQ
                     RRN"
     gene            13335..>13591
                     /locus_tag="OO130_002817"
     CDS             13335..>13591
                     /locus_tag="OO130_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5366149.1"
                     /translation="MNNSRLFRLSRIVIALTAASGMMVNTANAKEEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKPYYR"
BASE COUNT         3058 a         4029 c         3747 g         2757 t
ORIGIN      
        1 attttatatt tagtgtgttg ttttttatca gaataaataa cgtcttctga tgcgtttaaa
       61 acgtcagaaa gataaaaata tcatgtgaat aaaaaaagaa caggtagagc attaacatta
      121 tcttaaataa taaatagagg caaaaagatt attttctttt tgcgtttcct ttcaaatgaa
      181 aacgatcgtc gtctaaaatc agcagtaccc ccgacaaact cagggatttt gtgtataatt
      241 gcggcctttt tcggcaatct gccgtttttt ggcgcttttg ccctgctgac ttttgaggaa
      301 atccacatgt cattaccaca ctgcccaaaa tgcaactccg aatacactta cgaagataac
      361 ggcatgtaca tctgcccgga atgtgcctac gaatggaacg acacagaacc tgcacaggaa
      421 agcgacgagc tgatcgttaa agatgctaac ggcaatctgc tggctgacgg cgacagcgtt
      481 accatcatta aagatctgaa ggtgaaaggt agctcttcga tgctgaaaat tggcaccaaa
      541 gtgaaaaaca tccgcctggt tgaaggcgac cataacatcg attgcaaaat cgacggtttt
      601 ggtccgatga aactgaaatc tgagtttgtg aaaaagaact gattgtattg tgatcggtaa
      661 gccggataag gcgctcgcgc cacatccggc aacgatgccg actgcctgat gcgacgctga
      721 ccgcgcctta tcaggcttcc ctctcatctg tataaatttc gaactacact taactggctt
      781 ctcttaactg aggtcaccat catgccgtta agtccctacc tctcttttgc cggtaactgt
      841 tccgacgcga ttgcctatta tcaacgtacg ttgggcgcgg aactgctcta taaaatcagc
      901 ttcggcgaaa tgccaaaatc agcgcaggac agcgccgaga actgcccttc cggaatgcaa
      961 tttcccgata ccgccatcgc ccacgccaac gtgcgcattg ccggaagcga catcatgatg
     1021 agcgatgcta taccgtcagg aaaagccagc tactccggct ttacgctggt gctcgattcg
     1081 caacaggtcg aagaaggaaa acgctggttt gataaccttg ccgctaacgg aaaaattgaa
     1141 atggcctggc aggaaacttt ctgggcgcat ggcttcggca aagtcaccga taaatttggc
     1201 gtaccgtgga tgattaatgt cgtcaaacaa caaccaacgc aataacccgc cgggaggccc
     1261 gccctcccgc actgtcatcg aattcccgtt aactcttcat ctgttagtca cttttaatta
     1321 accaaatcgt cacaataatc cgccacgatg gagccacttt tttagggagg ctgcatcatg
     1381 caaacgatta tccgtgtcga gaagctcgcc aaaaccttca atcagcatca agcgctgcat
     1441 gcggttgatc tgaacattca tcacagtgaa atggtggctc tgcttgggcc gtcgggttcc
     1501 ggcaaatcca cacttttacg tcacttaagc ggtttgatta ccggcgataa atccgccggc
     1561 agccatatcg agctgctggg ccgcacagtc cagcgcgaag gccgtctggc gcgcgatatc
     1621 cgcaaaagcc gcgcccatac cggctacatc ttccaacaat tcaacctggt gaatcgcctg
     1681 agcgtactgg agaacgtgct gattggcgcg ctcggcagca cgccgttctg gcgcacctgt
     1741 tttagctggt tcacccgcga gcagaaacaa cgcgcgttac aggcgctgac ccgcgttggc
     1801 atggtgcatt ttgcccatca acgcgtttcc accctctccg gcggacagca gcagcgtgtg
     1861 gcgattgccc gcgcgctgat gcagcaggcg aaggtgattc tggccgatga acccatcgcc
     1921 tcgctggacc cggaatccgc ccgcatcgtg atggacaccc tgcgcgacat caatcagaac
     1981 gacggcatca ccgtagtcgt cacgctgcat caggtggatt acgccctgcg ctactgcgaa
     2041 cgcatcgtcg ccctgcgcca ggggcacgtt ttctacgacg gcagcagcca acagtttgat
     2101 aacgaacgtt ttgaccatct ctaccgcagc attaatcgca tcgaagagaa cgcgaaagct
     2161 gcctgacatc cccatcattg aggaaaacga atgaacgcta agataattgc ctcgctggcc
     2221 ttcaccagca tgttcagcct cagcaccctg ttaagcccgg cacacgccga agagcaggaa
     2281 aaggcgctga atttcggcat tatttcaacg gaatcacagc aaaacctgaa accgcaatag
     2341 acgccattct tacaggatat ggagaagaag ctgggcgtga aggtgaacgc cttctttgcc
     2401 ccagactacg caggcattat ccagggaatg cgcttcaata aagtggatat cgcctggtac
     2461 ggcaacctgt cggcaatgga agcggtggat cgcgccaacg gccaggtctt cgcccagacg
     2521 gtcgcggcgg atggatcgcc aggttactgg agcgtgttga tcgtcaacaa agatagtccg
     2581 atcaacaacc tgaacgatct gctggcgaag cggaaagatc tcaccttcgg caatggcgat
     2641 cctaactcca cctctggctt cctcgtcccc ggttactacg tctttgccaa aaacaatatc
     2701 tccgccagcg actttaagcg caccgtcaac gctgggcatg aaaccaacgc gctggccgtc
     2761 gccaacaagc aggtggatgt tgccaccaac aacaccgaaa acctcgacaa gctgaaaacc
     2821 tccgcgccag agaagctgaa agaactgaag gtgatctgga agtcgccgct gatcccaggc
     2881 gatccgatcg tctggcgcaa gaatctctcc gaaaccacca aagacaagat ctacgacttc
     2941 tttatgaact acggaaaaac gccggaagag aaagcggtgc tggaacgcct gggctgggcc
     3001 ccgttccgcg cctccagcga cctgcaactg gtgccgattc gccagctcgc actgtttaaa
     3061 gagatgcagg gcgtaaaaag caataaagga ctgaatgagc aggacaagct ggcgaaaacc
     3121 accgagattc aggcgcagct ggatgacttg gaccgcctga acaacgcgtt aagcgcgatg
     3181 agttcggtga gtaaagcggt gcagtaatgt gctcgatcgg tcccctctcc cttgagggag
     3241 agggttaggg tgagggggaa atgcgcacgc tgatgccctc atcccggccc tctcccacag
     3301 ggagagggag aaaacattaa acggagccaa acatgcaaac catcaccatc gccccaccca
     3361 agcgtagctg gttctcgctt ctgagctggg ccgttgttct cgccgtgctg gtcgtctcgt
     3421 ggcagggcgc ggaaatggcc ccgcttacgc tgattaaaga cggcggcaac atggcaacct
     3481 tcgttgccga cttcttcccg cccgatttca gccagtggca ggattacctc accgaaatgg
     3541 ccgtcacgct gcaaatcgcc gtctggggca ccgcgctggc agtggttctc tccatcccct
     3601 tcggtctgat gagcgccgaa aacctggtgc cgtggtgggt ttaccagccc gttcgccgcc
     3661 tgatggatgc ctgccgcgcc attaacgaaa tggtcttcgc catgctgttc gtggtcgccg
     3721 tcggtctcgg cccgttcgct ggcgtgctgg cgctatttat ccacaccacc ggcgtgctct
     3781 ccaagctgct ttccgaagcg gtggaagcga tcgaacccgg cccggtagaa ggcattcgcg
     3841 ccaccggcgc caacaagctg gaagagatcc tttacggcgt gctgccacag gtaatgccgc
     3901 tgctgatctc ctactccctc tatcgcttcg aatccaacgt ccgctcggcg accgtcgtcg
     3961 gcatggtcgg tgcaggcggg atcggcgtca ccctgtggga agcgattcgc ggttttcagt
     4021 tccaacaaac ctgcgccctg atggtgctta tcatcgtcac ggtcagcctg ctggatttcc
     4081 tctctcaacg gttgcgtaag cactttatct gataagcgag gcattgatat ctatgcactt
     4141 gtctacacat ccgaccagct acccaacacg ctatcaggag atagccgcaa aacttgagca
     4201 ggagcttcgt caacactatc gctgcggcga ctatcttccc gccgagcagc agctggcggc
     4261 gcgctttgag gtaaatcgcc acaccctgcg ccgcgccatc gaccaactgg tggaaaaagg
     4321 ctgggtacag cgccgtcagg gcgtcggcgt gctggtgctg atgcgcccgt tcgattaccc
     4381 gctcaacgcc caggcgcgtt ttagccagaa cctgctggat cagggcagcc atcccaccag
     4441 cgaaaaactg ctgtcggtgc tgcgcccggc atctggtcac gtcgctgacg cgctggggat
     4501 aaccgagggg gagaacgtca tccacctgcg caccctgcgc cgggtcaacg gcatcgcgct
     4561 ctgtttaatc gaccactact ttgcggacct caccctttgg ccgacgctgc aacgcttcga
     4621 cagcggctcg ctgcacgatt ttctgcgcga gcaaaccggg attgcgctgc gccgcagcca
     4681 gacgcggatc agcgcccgcc gcgcccaggc caaagagtgc cagcgtcttg aaatcccgaa
     4741 tatgtcgccg ctgctgtgcg tgcgcaccct taaccaccgt gacggtgaaa gcagcccggc
     4801 ggagtactcc gtcagcctga cgcgcgccga catgattgaa ttcactatgg agcactgaat
     4861 gcacgcagat atcgcgaccc gccagcactg gatgtccatg ctggcgcaca gccaaccggc
     4921 tgaactggca gcccgcctga acgcgctgaa catcaccgcc aactatgaag tgatccgcac
     4981 cgccgaaacc ggcctggtac agattcagcc gcgaatgggc ggtaccggcg aacgcttttt
     5041 tgccggcgac gccacgctga cccgcgccgc cgtacgcctg actgacggcc ccctcggcta
     5101 cagctgggtg ctggggcgcg acaaacagca cgccgaacgc tgcgcgctga ttgacgcgtt
     5161 gatgcagcaa tctcgccatt ttcaaaactt atcagaaacc cttattgccc cactggacgc
     5221 tgaccgtatg gcacgcattg ccgcacgcca ggccgaagtg aacgccagcc gggtcgactt
     5281 ctttacgatg gttcgcggag acaacgcatg accctggaaa ccgcttttat gcttcctgtg
     5341 caggatgccc agcacagttt tcgtcgcctg ttaaaggcca tgagcgagcc gggcgtgatt
     5401 gtcgccctgc atcagctcaa acgcggctgg cagccgctga atatcgccac caccagcgtg
     5461 ctgctgacgc tggctgataa cgacacgccg gtgtggcttt ccgcgccatt aagtaacgat
     5521 atcgtcagcc agagcctgcg ttttcatacc aacgcgccgc tggtcaatca gtcggaactg
     5581 gcgaccttcg cggtaacgga tgaggcgatt tccagcgaac agctcaacgc tctttccagc
     5641 ggcaccgctg ttgcgccgga agcgggcgcg acgctgattt tacaggtcgt cagcctgagc
     5701 ggcgggcgca tgctgcgcct tactggcgcg ggtattgccg aagaacgaat gatcgctccg
     5761 cagctgccgg agtgcattct gcacgaactc accgagcgcc cgcacccgtt cccgctcggc
     5821 atcgacctga tcctgacctg cggcgagcgg ctgctggcta tcccgcgaac cactcatgtg
     5881 gaggtgtgct gatgtacgtt gccgtgaaag ggggcgagaa ggagatcgac gccgcccacg
     5941 ccctgcaaga gagccgacgc cggggcaata ccgatttgcc tgaactgagc gtcgcccaga
     6001 ttgaacagca gcttaatctt gcagtagatc gcgtgatgac cgaaggcggc attgccgacc
     6061 gcgaactggc ggcgctggcg ctgaaacagg ccagcggcga taacgtcgaa gcgattttcc
     6121 tgctacgcgc ctaccgcacc acgctggcga agctggcggt aagcgagcca ctcgacacca
     6181 cggagatgcg tctcgaacgg cgtatttcag cggtttataa agatattccc ggcggtcagt
     6241 tgcttggccc aacctacgac tacacccatc gcctgctcga tttcaccctg ctggcaaacg
     6301 gcgaagcgcc gacgctgacc accgcagaca gcgaacaatc cccgtcgccg cacgttttca
     6361 gcctgctggc gcgtcagggg ctggcgaagt ttgaagagga tagcggcgca cagccggatg
     6421 acatcacccg cactccgcca gtttacccct gttcacgttc ttcccgtttg cagcagttga
     6481 tgcgcggcga cgaaggctat ttgctggcgc tggcctactc tacccagcgc ggttacggac
     6541 gcaatcaccc gttcgcgggc gaaatccgca gtggttacat cgacgtgtcg attatgccgg
     6601 aagagctggg atttgcggtg aacgtcggcg aactgctgat gaccgagtgt gaaatggtca
     6661 acggttttat tgacccgccg gatgagccgc cgcacttcac gcgcggctac gggctggtgt
     6721 tcggcatgag cgagcgcaaa gcgatggcgc tggttgaccg tgcgttgcag gctccggagt
     6781 acggcgagca cgcgacaggc ccggcgcagg atgaagagtt cgtgctggca catgccgaca
     6841 acgtcgaagc cgcaggcttt gtctcgcacc tcaaactccc ccactacgtc gatttccagg
     6901 ccgaactgga gctactcaaa cgtctgcaac aggagcagaa ccatggctaa tctgagcggc
     6961 tacaactttg cctacctcga cgagcagacc aaacgcatga tccgccgcgc catcttaaaa
     7021 gcggtggcga tccccggtta tcaggtgccg tttggcgggc gcgagatgcc gatgccctac
     7081 ggctggggaa ccggcggcat tcagctcacc gccagcgtga ttggcgaaag cgacgtgctg
     7141 aaggtgattg accagggcgc agacgacacc accaacgccg tgtcgattcg caacttcttt
     7201 aagcgcgtga ccggggtaaa caccactgaa cgtacggacg atgcgacgct tatccagacg
     7261 cgccatcgca tccccgaaac gccgctgacc gaagatcaga tcattatctt ccaggtgccg
     7321 atccccgaac cgctgcgctt tatcgagccg cgcgaaacgg aaacccgcac catgcacgcg
     7381 ctggaagagt acggcgtgat gcaggtgaaa ctgtatgaag atatcgcccg cttcggtcat
     7441 atcgccacta cctacgccta tccggtgaag gtgaacgggc gctacgtgat ggacccgtcg
     7501 ccgatcccga aattcgataa cccaaaaatg gacatgatgc ccgccctgca actgttcggc
     7561 gcggggcgcg agaagcgcat ctatgcggtg ccgccgttta cccgcgtgga aagtctcgat
     7621 ttcgacgatc acccgttcac cgttcagcag tgggatgagc catgcgccat ctgcggatcg
     7681 acccacagct atcttgatga agtggtgctg gatgacgccg gaaaccgcat gtttgtctgc
     7741 tccgataccg attattgccg ccaacagagc gaggcaaaaa accaatgaat caaccgttac
     7801 tttcggtcaa taacctgacc cacctttacg cgccgggcaa aggctttagc gatgtctctt
     7861 ttgatttatg gccgggggaa gtgctgggca ttgtcgggga gtccggctcc ggaaagacca
     7921 cgctgctgaa atcaatctcc gcgcgcctga cgccgcagca gggggagatt cactatgaga
     7981 gccgttcgct gtacgcaatg agcgaggccg accgccgtcg cctgctgcgc accgaatggg
     8041 gcgtagtgca tcagcatccg ctcgacggcc tgcgccgcca ggtgtcggca ggcggcaata
     8101 tcggcgagcg gctgatggcg accggggcac gtcattacgg cgatattcgt gccaccgcgc
     8161 agaagtggct ggaagaggtg gaaattcccg ccaaccggat cgacgacctg ccgaccacct
     8221 tttccggcgg catgcagcaa cgtttgcaga tcgcccgcaa cctggtgacg catccgaagc
     8281 tggtgtttat ggacgagccg accggcggac tggatgtgtc ggtacaggca cgcctgctcg
     8341 acctgctgcg cggcctggtg gtggagctga acctcgcggt agtgattgtc acccacgatt
     8401 taggcgtcgc ccgcctgctg gcggaccgtt tgctggtgat gaagcagggg caagtggtgg
     8461 agagtgggtt aaccgaccgc gtgctcgacg acccgcacca tccgtataca caactgctgg
     8521 tgtcatcggt tttgcagaat tgagccggtg ccggatgcgg cgtaaacgcc ttatccggcc
     8581 tacaaatgcg ctccccgtag gtcggataag acgcgtcagc gtcgcatccg acacccgaac
     8641 cacgaggcga aaaatgatta acgtacaaaa cgtcagtaaa accttcatcc tgcatcagca
     8701 aaacggcgta cgcctgcccg tcttacaacg tgcctcgctc accgtcaacg caggcgaatg
     8761 cgtggtgctc cacggtcact ccggcagcgg caaatcaacc ctgctgcgct cgctgtacgc
     8821 caactatcta cccgacgaag gtcaaatcca gatcaaacac ggtgacgagt gggtagacct
     8881 ggtcaccgcg ccagcgcgca aagtggtgga aatccgcaaa accacagtcg gctgggtgag
     8941 ccagtttctg cgcgtcatcc cgcgtatctc agcactggaa gtggtgatgc agccgctgct
     9001 cgataccggc gttccgcgtg aagcctgcgc cgctaaagcc gcgcgtcttc tcacccgcct
     9061 gaacgtgccg gaacgcctgt ggcacctggc accatcgacc ttctccggcg gcgaacagca
     9121 gcgcgtcaac atcgcccgcg gctttatcgt cgactacccc attctgctgc tcgacgaacc
     9181 taccgcctcg ctggacgcca aaaacagcgc cgcggtggtg gaactgattc acgaagccaa
     9241 agcccgtggc gcagccatcg tcggcatctt ccatgacgaa gctgtacgta atgacgtcgc
     9301 cgaccgcctg cacccaatgg gagcctcttc atgattatca ataacgttaa gctggtgctg
     9361 gaaaacgagg tggtgcacgg ttcgctggag gtgcaggatg gcgaaatccg cgcctttgcc
     9421 gaaagccaga gccgcctgcc agaagcgatg gacggcgaag gtggctggct actacccggc
     9481 ctgattgagc tgcataccga caacctcgat aaattcttca ccccgcgccc gaaagtcgac
     9541 tggcccgccc attcggcgat gagcagccac gacgcgctga tggtggcaag cggcatcacc
     9601 accgtgctga acgcggtggc gattggcgac gtgcgcgacg gcggcgatca gctggagaat
     9661 ctggagaaga tgatcaacac catcgaagag acgcagaaac gcggcgtcaa ccgcgccgag
     9721 caccgtctgc acctgcgctg cgagctgccg catcacacca cactgccgct gtttgaaaaa
     9781 ctggttcagc gcgaaccggt gacgctggtg tcgctaatgg accactcacc gggccagcgc
     9841 cagttcgcta accgcgagaa gtatcgcgaa tattatcagg gcaaatactc cctcaccgat
     9901 gcgcagatgc agcagtacga agaagagcaa ttggcgctcg ccgcacgctg gtcgcagccg
     9961 aatcgcgaat ccatcgccgc cctgtgccgc gcgcgacaaa ttgcgctcgc cagccacgat
    10021 gacgccacgc acgcccacgt tgccgaatcc caccaacttg gcagcgtgat cgccgaattt
    10081 accaccacgt tcgaagcggc ggaagcctcg cgtaagcatg gcatgaacgt gctgatgggt
    10141 gcgccaaata tcgtgcgtgg cggctcgcac tccggcaatg tggcggccag tgaactggcg
    10201 cagcttggtc tgctggatat cctctcttcc gactactacc ccgccagcct gctggatgcg
    10261 gcgttccgcg tcgccgatga cgagagcaac cgctttacgc taccgcaggc ggtgaggctg
    10321 gtgaccaaaa atccggcgca ggcgctgaat cttcaggatc gcggggtgat tggcgagggt
    10381 aaacgcgctg acctggtgct ggcgcatcgc cagggcaatc acattcatat cgaccacgtc
    10441 tggcgtcagg gtaaaagggt gttctgatga tgggaaaact gatttggtta atggggccgt
    10501 ccggctccgg gaaagacagc ctgctggcgg aactccgcct gcgggaacaa acgcagttac
    10561 tggtggcgca tcgctacatc acgcgcgacg ccagcgccgg aagtgaaaac catatcgccc
    10621 tgagtgagcg ggagtttttt acccgcgcgg ggcaaaacct gctggcctta agctggcacg
    10681 ctaacggtct gtattatggc gtcggcatcg agattgacct ctggctgcac gctggattcg
    10741 acgtagtcgt caacggttca cgcgcgcatt tgccgcaggc acgggcgcgc tatcaatcgg
    10801 cgctgctgcc cgtctgttta caggtttcgc cggagatcct gcgccagcgc ctgaaaaacc
    10861 gtggccgtga aaacaccagt gaaattaacg cccggctggc gcgcgccgcc cgctatactc
    10921 cacaggattg ccatacgctc aacaatgacg gcagcctgcg ccagtcggtc gacacactgc
    10981 tgacgctgat tcatcagaag gagaaacacc atgcctgctt gtgagcttcg cccggccacg
    11041 cagtacgata ccgacgcggt ttacgcgctg atttgtgagc taaaacaggc ggagtttgac
    11101 caccacgcgt ttcgcgtggg ctttaacgcc aacctgcgcg acccgagcat gcgctacccg
    11161 agcatgcgct accatctggc gctgctggat ggcgaagttg tcggcatgat cggcctgcat
    11221 ttgcagtttc atctgcatca tgtcaactgg atcggcgaaa ttcaggaact ggtggtgatg
    11281 ccgcaggcgt gcggtctgaa cgtcagcagt aagttactgg cgtgggcaga agaagaagcc
    11341 cgccaggccg gggccgaaat gaccgaactt tccaccaacg tgaagcgcca cgacgcgcac
    11401 cgtttttacc tgcgtgaagg ctacgagcag agccacttcc gcttcaccaa ggcgctgtaa
    11461 catgagcctg accctcacgc tcaccggcac cggcggcgca cagggcgttc cggcatgggg
    11521 ctgcgagtgc gtagcctgcg ccagagcgcg gcgctcaccg cagtatcgcc gccagccatg
    11581 cagcggcgta gtgaagttta acgacgcaat caccctgatc gacgccgggc tgcacgatct
    11641 cgccgatcgc tggccgcccg gatcgttcca gcagtttttg ctgacgcact accatatgga
    11701 tcacgtgcag gggctattcc cgctgcgctg gggcgtgggc gatccgatcc cggtttacgg
    11761 cccgccggat gaacagggct gcgacgatct gtttaaacat ccgggcctgc ttgatttcag
    11821 ccacacggtg gaaccgtttg tggtgtttga tttgcagggg ttacaggtca cgcccctgcc
    11881 gctcaaccac tcaaaactga ccttcggtta tctgctggaa acggcacaca gccgggtggc
    11941 gtggctgtct gacaccgcag gcttgccgga aaaaacgctg aaatttttac gcaataatca
    12001 gccgcaggta atggtgattg attgcagcca cccgccgcgc gaggatgcgc cacgtagtca
    12061 ctgtgattta aacaccgtgc tggcgctgaa tcaggttatc cgctcgccac gggtgattct
    12121 gacccatatc agccaccagt ttgatgcgtg gctgatggaa aacgcactgc cgtcggggtt
    12181 tgaggcggga tttgatggga tggagattgg ggtggcgtga tgagagggaa tgtgcgcgct
    12241 ggccccctca ccctaaccct ctccccagag gggcgagggg accgattgtg ctcgatattg
    12301 aatattgcgc tggttttctc cctctcccca ttggggtgag gggcgatgcc tgctccatac
    12361 ccaacctcat cgcccatact catcttccat tctccgctct tcatcttcta gctgccgacg
    12421 ctcctgatca agctggcgct ggcgatcgtc cagctgcctg cggcgatctt caaactggcg
    12481 gcggcggtcg tcatattgtc tgcgccgatc gtcgctcact tcacgctgcc agccgtcgtc
    12541 gcgcgaatct tcatagtctc gcccacggtc agggttataa gcgtcgttaa tcgcctgctg
    12601 aatattgcca atggtgtcgt cgataatatc ggcctgggcc ggaacggtgg acagcgtgag
    12661 cagggtgaat aaaagaaata gcggaaagcg tttcattagc caacctcaaa aagaaactct
    12721 atccacatta atcattactc atccatgcaa gtagtggatg aatctcaatt tctccgctgc
    12781 tctattgccg taatcgcctc cacgcgttgt tgatgacgac cgccttcgta ctgtgcgccc
    12841 agccacgcat ccacaatcat ttttgccagt tcgaggccaa ccactcgtga accaaaagcc
    12901 agtgcaactt cactggcata atgcggataa tcagtgcgct ctgacgacct ggtgtcttta
    12961 tcaatcacct cactcattga ccatgaataa aaggctggca cagcaacgca gaatcctgcg
    13021 gtgccagcga catgaccgcc cattgctccg gtacgttttt taaaaagaaa caccggttgc
    13081 attgataaat tgtgctcagt atttgccgaa gtacaatata tataataaat atataaaaca
    13141 cctcatatta tttacagaga taataaattc gcgtaatttt ctctcttcag cacaaatgcc
    13201 taataaaatg gatcgttcag ctcacacttc cacttattaa agtcgcaaat tcccaattat
    13261 gataacccca gctttaaatg acgtattttg tatggattac atatttacga cataaatgat
    13321 aaatggagat ttatatgaat aactctcggt tattccgttt gagcagaatt gttattgcgt
    13381 taactgccgc cagcggcatg atggtaaata ccgctaacgc gaaagaggaa gcgaaagccg
    13441 ccactcaata tacccaacag gttaatcaga attacgccaa atcattaccg tttagcgatc
    13501 gtcaggattt tgacgacgct cagcgtggat ttatcgctcc gctgctggat gaaggtattc
    13561 tgcgcgatgc taatggcaaa ccatattatc g
//
LOCUS       DAJTMC010000107        13584 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.107, whole genome shotgun sequence.
ACCESSION   DAJTMC010000107 DAJTMC010000000
VERSION     DAJTMC010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13584
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.107"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(241..1614)
                     /gene="cobA"
                     /locus_tag="OO130_002818"
     CDS             complement(241..1614)
                     /gene="cobA"
                     /locus_tag="OO130_002818"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCR5366150.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFTTMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            complement(1633..2439)
                     /gene="nirC"
                     /locus_tag="OO130_002819"
     CDS             complement(1633..2439)
                     /gene="nirC"
                     /locus_tag="OO130_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HCR5366151.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKSNRPVADKFNQTETAAG"
     gene            complement(2566..2892)
                     /gene="nirD"
                     /locus_tag="OO130_002820"
     CDS             complement(2566..2892)
                     /gene="nirD"
                     /locus_tag="OO130_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HCR5366152.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            complement(2889..5432)
                     /gene="nirB"
                     /locus_tag="OO130_002821"
     CDS             complement(2889..5432)
                     /gene="nirB"
                     /locus_tag="OO130_002821"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HCR5366153.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRIFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            complement(5694..6875)
                     /gene="tsgA"
                     /locus_tag="OO130_002822"
     CDS             complement(5694..6875)
                     /gene="tsgA"
                     /locus_tag="OO130_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HCR5366154.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSTA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            7146..7718
                     /gene="ppiA"
                     /locus_tag="OO130_002823"
                     /gene_synonym="rotA"
     CDS             7146..7718
                     /gene="ppiA"
                     /locus_tag="OO130_002823"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HCR5366155.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGAPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            7823..7990
                     /locus_tag="OO130_002824"
     CDS             7823..7990
                     /locus_tag="OO130_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HCR5366156.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLVELRRHYER"
     gene            7980..8582
                     /locus_tag="OO130_002825"
     CDS             7980..8582
                     /locus_tag="OO130_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HCR5366157.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKTKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            8614..9177
                     /gene="pabA"
                     /locus_tag="OO130_002826"
     CDS             8614..9177
                     /gene="pabA"
                     /locus_tag="OO130_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HCR5366158.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHNGEGVFRGLANPLTVTRYHSLVVEPGSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHL"
     gene            9263..10483
                     /gene="argD/dapC"
                     /locus_tag="OO130_002827"
     CDS             9263..10483
                     /gene="argD/dapC"
                     /locus_tag="OO130_002827"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HCR5366159.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTASTPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMYYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPHYKGRARDFLYAGAEVGVMVLNAGPDVMRLAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(10550..12640)
                     /locus_tag="OO130_002828"
     CDS             complement(10550..12640)
                     /locus_tag="OO130_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCR5366160.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQLLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(12691..13323)
                     /gene="crp"
                     /locus_tag="OO130_002829"
     CDS             complement(12691..13323)
                     /gene="crp"
                     /locus_tag="OO130_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HCR5366161.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
BASE COUNT         3288 a         3495 c         3557 g         3244 t
ORIGIN      
        1 tccctatgta ttatatatta attaatattc attgattata tgaatataag cagactgctc
       61 gttaagaacg atacaggaat atttaattgg cgtcagtgag cgaatgactt aattttgtaa
      121 gtcgctgtaa cggtgaatat aacccattag cagataatat ttattaaggg gttatgtttt
      181 atttgctgaa tatataaaaa gagtcattaa gaaggccaga attctctggc ccgggttaaa
      241 ttaatggttg gagaaccagt tcagtttatc gcgcaggcca acaacccgac caataataat
      301 tagcgatgga ctgttcattt gctgagcaag ttcaccgagt tgcgtgagcg taccgtcaat
      361 cacgcgctgc gtgactgccg ttccgttttc gacaattgcc accggcattt cgccaggcat
      421 accgtgttca atcagctttt gctgaatagt cgcggcctga ttcaacccca tatagaacac
      481 cagcgtctgt ttttctgccg ccaggttttc ccagtccagc tcgccaccgg tttttaagtg
      541 tccggtaatt aagcgtacgc tctgggcata atcgcgatgc gtgagtggaa tacccgaata
      601 ggcagagcaa ccagaagctg cggtaatacc cggaaccacc gagaacggaa tacccgcgtt
      661 gcacagtgtt tccagctctt cgccaccacg gccaaaaata aacggatcgc cacctttcag
      721 ccgcaccacg cgtttgcctt tttgcgcttc ccgcagcagg atctggttaa tctcttcctg
      781 cggtacacag tggtatcccg cgcgtttgcc gacgaaaacg cgatcagcat cgcggcgtac
      841 cagattcata atatcgtcag aaaccagacg gtcgtagacc accacatctg cctgctgaat
      901 ttgttgcagt cctttcagcg tcagcagccc ggcatcgccc ggacctgcac caaccagcac
      961 cacttcaccg cgatggtcga gcggttcgtt gattaactgt tcggtcgttt cagtaatggc
     1021 tttctgatcg ttgtttgcca gcgactgcgc caggcggtcg ttaacgaaca atttctccca
     1081 gaaacggcga cgctcactca tcgtggtgaa ctgttgtttc actcgcccgc gtaattgccc
     1141 ggcatatttc gccacctggc ccagatgcaa cggcagcagt gattcgagtt tttcgcgcaa
     1201 caggcgcgcc agaaccggag aggtgccgcc agaggagacc gctaccatta gcggtgagcg
     1261 gtcaataatc gacggcataa taaagctggc ggctttcggc gcatcgacta cgttacagaa
     1321 gatgcgacga gcttcagcgg cttcgctgac gcgctggtta agcgtgtcat catccgtcgc
     1381 tgcaatcgcc agccagcagg tgtcgagaag gctttcatca aatggccctt cgacgagggt
     1441 taacatgcct gcatctgccc atgcggtgaa ctgtggaata aacgctaatg cattcaccgt
     1501 taagcgagcg cctgcgtcta acagcaacct tgctttgcgt tccgcgacat caccaccgcc
     1561 gacaatcaga cagtcgcgat cgcgtaattg gcaaaatata ggcaaatgat ccacgtaaaa
     1621 accccttagt aattaaccgg cagccgtttc agtttgatta aatttgtccg caaccggacg
     1681 attcgatttc ggcgtagcat accaataacc caatcccatg aatacggcac ctgataaagt
     1741 attacccagc gtcacccaca gcaggttatg accaataccc gccagcgtgt aggcttcgct
     1801 gtggttgccg aaccaggaga gcgcgaacag cgtcatgtta gcgatagagt gctcgtagcc
     1861 ggacgcgata aatgccagca gacaccacca gatagcgata aatttcgccg ccccttcagt
     1921 gcgcagcgcc atccagattg ccaggcaaac cagccagtta cacaatgcac ctttgaagaa
     1981 gagtaccatt gccggtgcag tggttttagc cagcgcgacg gagtgaacga tgctggtatc
     2041 taccggcagc aggctaccgc cgccccagct atagagcatg gcaacgaaga cggaaccgac
     2101 caggttaccc agccaggttt gcggcaggat tgcccacatt tgcccgtggc tgatgctgcc
     2161 cgcttttacc ccaaaggtga ggaacatggt gtgtccggtg aacagttcag aaccggcgat
     2221 aatcaccagc gttaaggcga taccaaaggt cgcgcccatc accagcggac gtacggacgg
     2281 gtcgagcaaa ttaccgagcg tgaaaatcaa gatgatccca agacccacat acgcgcccgc
     2341 catggcggag ctgacccaaa agccgagcgg gttatttgcc gacaggcgtg caatgcgcgc
     2401 agcgttagcc gcacacttat taatagtgtc tgtaaacatt tgattatcct ttaaaaaata
     2461 aaaacaaaaa aataaaacaa aaagtttaaa aaatgtcctg caatataccg aaggtaatta
     2521 caggaccatg caaaaagggg aggcattgcg cctcccgtta aaacattaac cgcgcagctg
     2581 caccacgccg tctttcactc gtgcttcgta atgtttgacg gaaaactgtt cgtcttccat
     2641 gcacaagcca tcacttaagc gaaaacgctg ttttttcagc gggctggcga cccacagctc
     2701 gccctggtgt tccgcaatca gcccgcgtga cagcacgctg gactcgaaga acggatcgat
     2761 gttgctgatc gcaaacacct ggtcgctgtg atacgggcgg aaaatcgcga cctgctcgtc
     2821 gcctaacagc gcgcagacgc cggtttcagg caggatgtca tcgattttgc agatgtcttt
     2881 ccactggctc atgcgttgtc ctccaccaga gttaccggga tacgttcata cggcgttgcc
     2941 ggacggtgct gttcgcgctc tggcaccatc tgcacgttcg ggtcacgctt gtcgctgttg
     3001 atgaagtgtt tgaagcgagt ctgcgcggac ggtgtattga ccgtttcagt ccactcacac
     3061 actaccgctt cacgcaggcg cgccatctct tcttccagat gagcgttcag ccccagtttg
     3121 tcgtcaatga tcaccgcttt caggtaatcg atgccgcctt cgaggttttc taaccacggc
     3181 gcggtacgcg tcagtttgtc ggcggtacgg atgtagaaca tcatgaagcg gtcgagatat
     3241 ttgatcagcg tttcgcgatc gatatcagct gccagcagat ccgcatgacg cggtttcatg
     3301 ccgccgttgc cgcaaacata caggttccag cctttttcag tggcgataat acccacgtct
     3361 ttaccctgag cttctgaaca ttcacgggta cagccggaga caccgaactt cattttgtgc
     3421 ggcgtacgga tgcctttgta gcggttttcc agttccacgc cgaggccgac gctgtcgcca
     3481 acgccgtagc ggcaccaggt gctacccacg caggttttcg ccatacgcag tgctttcgca
     3541 taggcatgac cggtttcgaa gccggcttca atcagctgac gccagatctc tggcagatcg
     3601 tctttctgtg cgccaaacat cgccaggcgc tgggaaccgg tgattttggt gtagagatta
     3661 aattcacgcg cgatacgacc cactgccatc agcccttccg gggtgatttc accgcccgga
     3721 gaacgcggga tcaccgagta ggtcccgtct ttctggatgt tagcgaggaa gttgtcgtta
     3781 gaatcttgca gcggagtatg ttctggcttc agaatgtatt cgttccagca ggaggccagc
     3841 agcgaaccga cggtcggttt acaaacttca caaccgtagc ctttgccgtg tttcgccagc
     3901 agttcttcga aggttttaat gccttcaacg cggatcaaat ggaacagttc ctgacgcgaa
     3961 taagcaaagt gttcgcacag gttgttgtta acttcgatgc cctgtttcgc cagttccgcg
     4021 ttcagtacct gagtgaccag cgggatacag ccaccgcagc cagtacccgc tttggtttca
     4081 gctttcagcg ccgcaactgt gtggcagcct ttgttgatgg cagcaatcag atcaccttta
     4141 gtgacgtcga agcaggagca gatttgcgcg ctgtccggca gtttatcaac accgatagac
     4201 ggcttaccgc tacccgagtg cgccggcaga atcagagaat ccgggttttc cggcagctcg
     4261 atagcattca gcaccagttg cagcaagtta ccgtagtcgc tggtatcgcc caccagtacc
     4321 gcaccgagca gggttttgtt gtcttcgctg acgatcaggc gtttgtagat ctctttgctt
     4381 tcgtcgaggt aaacgtagct acgtgcgcca ggcgtgcgac catgagcatc accaataccg
     4441 cctacgtcca cgcccagcag tttcagcttg gcgctaaggt cagcaccttc aaaggcgttt
     4501 tcgctaccga gaatatggtc aacggcgacc tgcgccattt tgtagccagg tgctaccaga
     4561 ccaaatatac ggttgttcca gcttgcgcat tcaccgatag cgtagatatc cggatcggaa
     4621 gtttggcagg aatcattaat gacgataccc ccacgcggag caacgtccag accacactgg
     4681 gttgccagct tatcgcgcgg acggataccg gtagagaaga cgataaagtc gacttccagt
     4741 tcgctgccgt cggcaaaacg catggtttta cgtgcttcga caccttcctg cacaatctca
     4801 agggtgtttt tgctggtgtg aacgcgcacg cccatacttt cgattttgcg acgcagctgc
     4861 tcgccaccca tctgatcaag ctgttccgcc atcagcatag gggcaaattc gataacgtgg
     4921 gtttcaatac ctaagttttt cagcgcgcca gcggcttcca gacctaacag gccgccacca
     4981 acaacggcac cgcgtttgct gcgacgggcg caggattcaa tggcgttgag gtcttcaatg
     5041 gtgcgataga caaagcagtc ctgagtatca gaacctttga ttggtgggat ccacgggtag
     5101 gaaccggttg ccatgatcag cttgtcataa aaaacggtac gtccggcgct ggagtgaatc
     5161 accttctcct gacggttgat ggtgatagcg cgttcgccga ccagaacttt gatgccgtgt
     5221 ttctcgtaga agccttcgcg caccagcgac agctcttcgg cggtgtggtg agagaagtaa
     5281 gacgagaggt gtacgcggtc ataagcgatg cgcggttctt cacagaaaac ggtaatatca
     5341 aagttggccg catcagattt atcaagaaga tcttcgataa agcgatggcc gaccataccg
     5401 ttaccgataa ttgcgagtct gactttgctc atttttgcct cgatttcttt tctattaccg
     5461 cctaccttaa cgattcagca accccgctta ttgatgtaaa tcaaattcac ctttatatac
     5521 cccttaaggg gtatgttgct gatttgtata aattttctta agtcacggaa ttgtctgaca
     5581 tttcatgttt gtgcaaaaaa tagacaaaat gaacgggttt atagaaatag atgcacgtag
     5641 cggggttaga gaggaaaagg ccggacgacg atagtgccgt ccggcattag caattaatga
     5701 gaggtcaggg tgttatgctg acggtgacgg ctgacgaaac ctaacaggaa gcacatcaca
     5761 aagacgacag cgtacagacc gtttgctgta agcagtgccg cctgcggacc gctatgttca
     5821 acaatcgggc cggtaaccac aaaggtcaac atagtaccga tggttccgca ggtcaggaca
     5881 aagttaacca gttttggcga cggtactttg gtctgctgtg aacccagggt gatgatggtg
     5941 gtatagatcg cgctggagaa gaagcccaga gcgagaattg accacgccat atgtgctggt
     6001 gttccggtgt taaagacgta catcagaatc gcagccagac cagccagtac ggtcagaatg
     6061 cgttgcaaat caaagaagcg aagaataaag ctgaacgccc acatgccgac catgtatgac
     6121 atccagaagt tactcaccag cgtgcccgcg tcgttcaggc tcatgcccag gcctttggca
     6181 tactcaggca cccaggagat aaaacctaac tgaccgagga tgtagcacag cgccgcaaca
     6241 gagagaaaca gtacgccgat cccccacttt tctttctcta ccggagcatc cgtttttggt
     6301 gcatgtttgc ccagtgctgg gaactcacag ccgaaggtca gaataaaaat agcgacatac
     6361 accagcccga tgcaggcata aacccagtac cactcaatgc tgcgcgccag tagaaacgcg
     6421 gcgatcattg ggaaaatcat cccagccata ctgaagaagg agtcagtaaa taacaggcgg
     6481 gaaccgcgct gacgcccttc atacatttgt gttatcagga atgtaccaat cgacatagta
     6541 atgccgctga ccaccccgag aatgaacatc gccgtcgaga acagcgccag gctgtggctg
     6601 aacatcaaac cggcaaccgc cagcaccatc aggagaaagc caaaacgtaa ctgcgttttc
     6661 aacgggacga tttccatcag ccaggcgttg aggaagatag agattaaaat gccggcgttg
     6721 aggaaggtga aggtgttact catactggaa acaggcagat tgaaataatc ggcgatattt
     6781 cccatcacca tcccggtgac aataaccaac gcaccagtca gtgcgtagga gagaaagcta
     6841 atccatgtga gcttgatgcg attgctgtta gtcatgattg gcctgcgttc aaaaataaaa
     6901 tggcatagcg ggatatgccg cgagcgggtg attttaggtg attttgtgat ctgtttaaat
     6961 gttttattgc aatcggttgc taaattgcat tttaagaagt gatcttgatc acggaataaa
     7021 aagtgatcgt caggttacat atatttcaga tacgtaaaat taggtaaagg gatggccttg
     7081 ttcttgaagg ctatttagaa tctcttcact tgcttttttt ctgctctgtt tgttaaggaa
     7141 atctcatgtt caaatcgacc ctggcggcga tggctgctgt tttcgctctt tctgctcttt
     7201 ctcccgcagc aatggcagcg aaaggggccc cgcacgtatt gttgacaacc tcagctggta
     7261 acatcgaact ggagctggat aaacaaaaag cgccagtgtc tgtgcaaaac tttgtcgatt
     7321 atgtgaacag cggtttttat aacaacacta cctttcaccg cgtcattcct ggctttatga
     7381 ttcagggcgg cggtttcacc gagcagatgc agcagaaaaa accaaacccg ccaatcaaaa
     7441 atgaagccga taacggcctg cgcaacacgc gtggcaccat cgcgatggcg cgtaccgctg
     7501 acaaagatag cgccaccagc cagttcttta tcaacgttgc cgataacgcc ttccttgacc
     7561 atggtcagcg tgatttcggt tacgcggtat ttggtaaagt ggtgaaaggc atggacgtgg
     7621 ccgataagat ttcccaggtg ccgactcatg acgttggtcc gtaccagaat gtgccgtcaa
     7681 aaccggtagt tatcctttcc gctaaagtcc tgccgtaatg atttctcgcg cgggcaatct
     7741 tgcccgcgct tctgctctcc cggcgtaacc cggatttgcc gcttatactt gtggcaaatg
     7801 gacacgttca gggaggcatc aagtgaagaa actcaccgat aagcaaaagt cccgtctctg
     7861 ggagcttcag cgtaatcgta atttccaggc cagtcgccgt cttgaaggcg tcgagatgcc
     7921 tttagtcact cttactgccg cagaggcttt agcgcgcctt gtagagctga ggaggcacta
     7981 tgagcgataa attcggcgaa gggcgcgatc cgtatcttta tccaggcctt gatatcatgc
     8041 gtaaccggct gaacatccgc cagcaacaac ggctggaaca ggccgcttac gaaatgacgg
     8101 cgctgcgtgc tgcgaccatt gagcttggtc cgctggtgcg cggtttaccg catttgcgaa
     8161 ctatccatcg ccagctgtat caggatattt tcgactgggc agggcaactg cgtgaagttg
     8221 atatttatca gggcgatacg ccgttctgcc actttgctta tatagaaaaa gagggcaatg
     8281 ccctgatgca ggatctggag gaagaaggtt atctggttgg cctggagaaa acgaagttcg
     8341 tcgagcggct ggcgcattac tattgtgaaa tcaacgtgct gcatcccttc cgggtgggaa
     8401 gtggtctggc acagcggatc ttcttcgagc aactggcgat tcatgccgga tatcaactga
     8461 gctggcaggg tatcgaaaaa gaggcctgga atcaggcaaa tcagagtggg gcaatggggg
     8521 atctcaccgc actgcagatg atatttagca aagtggtaag cgaagccggg gaatctgagt
     8581 aaaatagcgc ggttctttcg taccggagcc gccatgatcc tgcttattga taactacgat
     8641 tcttttacct ggaacctcta ccagtacttt tgtgaactgg gggcggatgt gctggttaag
     8701 cgcaacgatg cgttgacgtt ggcggatatc gacgccctta aaccacaaaa aattgtcatc
     8761 tcacccggcc cctgtacgcc agatgaagcc gggatctccc ttgacgttat tcgccactat
     8821 gccgggcgct tgccgattct tggcgtctgc ctcggtcatc aggcaatggc gcaggcattt
     8881 ggcggtaaag ttgtgcgcgc cgcaaaggtc atgcacggca aaacctcgcc gattacacat
     8941 aacggtgagg gcgtatttcg ggggctggca aatccactta ccgtgacacg ctaccattcg
     9001 ctggtggtgg aacctggctc attaccagcg tgctttgacg tgacggcctg gagcgaaacc
     9061 cgcgagatta tggggattcg ccatcgccag tgggatctgg aaggtgtgca gttccatcca
     9121 gaaagtattc ttagcgaaca aggacatcaa ctgctggcta atttcctgca tctctgattt
     9181 ccgattgcca ttcagtgatt ttttatgcat attttgtgat tataatttca tatttattta
     9241 tgcgtaacag ggtgatcatg agatggcaat tgaacaaaca gcaattacac gcgcgacttt
     9301 cgatgaagtg atcctgccga tttatgctcc ggcagagttt attccggtaa aaggtcaggg
     9361 cagccgaatc tgggatcagc aaggcaagga gtatgtcgat ttcgcgggtg gcattgcagt
     9421 tacggcgttg ggccattgcc atcctgcgct ggtgaacgcg ttaaaaaccc agggcgaaac
     9481 tctgtggcat atcagtaacg ttttcaccaa tgaaccggcg ctgcgtcttg ggcgcaaact
     9541 gattgaggcg acgtttgccg aacgcgtggt gtttatgaac tccggcacgg aagctaacga
     9601 aaccgccttt aaactggcac gccattacgc ctgtgtgcgt catagcccgt tcaaaaccaa
     9661 aattattgcc ttccataacg cttttcatgg tcgctcgctg tttaccgttt cggtgggtgg
     9721 gcagccaaaa tattccgacg gctttgggcc gaaaccggca gacatcatcc acgttccctt
     9781 taacgatctc catgcagtga aagcggtgat ggatgatcac acctgtgcgg tggtggttga
     9841 gccgatccag ggcgagggcg gtgtgacggc atcgacgcca gagtttttgc agggcttgcg
     9901 cgagctgtgc gatcaacatc aggcattatt ggtgtttgat gaagtgcagt gcgggatggg
     9961 gcggactggc gatttgtttg cttacatgta ctacggtgtg acgccggata ttctgacttc
    10021 tgcgaaagcg ttaggcggcg gcttcccgat tagcgccatg ctgaccacgg cggaaattgc
    10081 ttctgcgttt catcctggtt ctcacggttc cacctacggc ggtaatcctc tggcctgtgc
    10141 agtagcgggc gcggcgtttg atatcatcaa tacccctgaa gtgctggaag gcattcaggc
    10201 gaaacgccag cgttttgttg accatttgca gaagatcgat cagcagtacg atgtgtttag
    10261 cgatattcgc ggtatggggc tgttaattgg cgcggagctg aaaccacact acaaaggtcg
    10321 ggcgcgtgat ttcctgtatg cgggtgcaga ggttggcgta atggtgctga atgccggacc
    10381 ggatgtgatg cgtttagcac cgtcgctggt ggtggaagat gcggatatcg atgaagggat
    10441 gcaacgtttc gcccacgcgg tggcgaaggt ggttggggcg taaataagac gcatcaggca
    10501 aaatatgtcg gatgcggcgt gaatgcctta tccgatggtt tagcctgtat tacgccttcg
    10561 aatcccgcaa cttgcgactc agccaaatcc cgtgatgcgg tcgctgacgc catgccatcg
    10621 acgaaatggt gtgcatggtg ttcagatgac caataacccg ctgtaaatgc tgctccagcg
    10681 tacctgccgc gccttcgtgc ggctgcatct ccggcgcatc catgatattg gcatcgccag
    10741 aactacccgg ttcgtcatac tccagtcgct gctgacaacg ctgaatggcg atttcacaag
    10801 actgtaaata ctcttgtgcc agttcaggtg gcaatgcccg gtgttcccgc gccagcgtgg
    10861 tcatggcatt aatatgctca acaataaact ggctgtgcgt tacccacagt ttcatatctg
    10921 ccagataatg gctgttaaac gccggttcct gcatcgcctg attcaatgag ttatacagag
    10981 tgttatgtgc ctgatttacc cgcattcgct gccaggccag tggcgtaggt tgcggatcct
    11041 cgctaagaat caagcgaatc gcttcctgat aggcttctaa agcgtcatgg gcgtttttac
    11101 gcaataaccc gctctgccac tgcggccaca gccagacagt accgccgaaa gcaattaaac
    11161 aaccaataat ggtatcgata agacgcggaa ggatgtattg ctcgccgttc aaccacaata
    11221 gttgcagggt atacactgcg gtaatagtaa aaccgaccgt cgcccagccg tagtttttgc
    11281 gcaatatcag gtagctggcg agggtggtaa tcagcatcaa cgtcagggtg taaccttcgg
    11341 gaattttaaa gtgcagcgcc acgcccgcaa tgattaaccc gaccacggtt cccacggagc
    11401 gattcacaat cctcagacgg gttgcgccat agccattttg tgtcaccagc aataccgtca
    11461 tcaggatcca gtacgacttc ggcagatgca gcgcggtgcc catcaggctg gcaacgctta
    11521 acatcacact gagtcgtccg gcattgcgta gcgccggaga ctttagtgac agataacttt
    11581 tcagcgcagg aagtaatggc attcgccgct gtttatcggc cagtaagtca cgggcataga
    11641 gcggtttttg ggtgcgcagc acgcgggcga tgcggctgaa atgccagtag cagaattgcc
    11701 caaccggatt atccggatgc tggcgggcga ttttttccag tgcgccaatt tgcttttcca
    11761 tcgtaaaacg cgttggcagg cgatggtaaa gaatgtcatc agccagcacg cgcaggcgag
    11821 cggcgacggt ttgcgcattc cagcggataa cttcttccgc atggctacgc tcgaccagct
    11881 tttgcacctc ttccggctga tgcaaactga ccgaaatatg ttcctgtaaa tccagcgcct
    11941 cctggaaaat acgcagcatc cgcttgtagt cagtattatt ttgcgcggaa agcatatgca
    12001 tttgctgata gcactgggta attagatcga ccgctttttg ctggcgcacc agcagcggcg
    12061 gcagcgcttt ttcagggtcg gtgtgctggg taagcaggct gtatttggct tcacaataat
    12121 ctgccagttc acggtacagc agacttagtg actcgcgcag cggttgttcg cgccagatcc
    12181 agaaccaaaa ccagttaaac aatccgtacc agagagtgcc caacgcatag atgagcaacg
    12241 gttcccagac cggcatgtat cccgccagac tgagggtaaa aatggctgcc agcagcgatg
    12301 cgggaagcaa ttttgcgtgc aatggcccca actcagcagt gacgccaagt accagcgtta
    12361 atccggtcag caaaaagggc aggggaacat cttttgccag tagcagctgt gtcagtaagc
    12421 tacaggtggc aaacagcgac gcaccaatga ttaagcgttt gaaaaaacgt ttatgaggcg
    12481 tatcaaggcc cgcaatattg caacaggcag gaacgagaga gaagagcaga ccgaatcgta
    12541 attcgccaag cattaaccca acggccacgg gcaaacatag caccagcgtt tgtcgaagtg
    12601 catagttgat atcggggtga taaatcagtc tgcgccacat cgggggaaac aaaatggcgc
    12661 gctaccaggt aacgcgccac tccgacggga ttaacgagtg ccgtaaacga cgatggtttt
    12721 accgtgtgcg gagatcaggt tctgatcttc aagcatcttc agaatgcgtc ccacggtttc
    12781 acgagaacag ccgacaatct gaccgatttc ctgacgggta attttgattt gcataccgtc
    12841 cgggtgagtc atagcgtctg gttgttttgc cagattcagc agagtctgtg caatgcggcc
    12901 cgtcacgtcg aggaacgcca ggttgcccac tttctctgaa gtgacttgca gacgacgcgc
    12961 catctgtgca gacagacgca tcagaatgtc cgggtttacc tgaatcaatt ggcgaaattt
    13021 tttgtacgaa atttcagcca cttcacaggc ggttttcgca cgtacccatg cgctacgttc
    13081 ctggccctct tcaaacaggc ccagttcgcc aataaaatca ccctgattca gataggagag
    13141 gatcatttct ttaccctctt cgtctttgat cagcactgcc acagagcctt taacgatgta
    13201 gtacagcgtt tccgcttttt caccctggtg aataagcgtg ctcttggatg ggtacttatg
    13261 aatgtggcaa tgagacaaga accattcgag agtcgggtct gtttgcggtt tgccaagcac
    13321 catgcgcggt tatcctctgt tataagcttt ctccagagcc agataacgcc gctgtctctg
    13381 gattgccgaa atatgcttcc cgctacctgg gaaggggcta tcaactgtac tgcacggtaa
    13441 tgtgacgtcc tttgcataca tgcagtacat caatgtatta ctgtagcatc ctgactgttt
    13501 tagcatagct ttcgctttgt gtctcctggt gtctcgcttc agcatgaccc aggtcgcctt
    13561 ccgttgcgcg atttggttag tacg
//
LOCUS       DAJTMC010000108        13520 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.108, whole genome shotgun sequence.
ACCESSION   DAJTMC010000108 DAJTMC010000000
VERSION     DAJTMC010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13520
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.108"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            <1..186
                     /locus_tag="OO130_002830"
     CDS             <1..186
                     /locus_tag="OO130_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR5366162.1"
                     /translation="CWSLAAGANPSFIASQMGHTNAQMVFNVYGAWMKDNNHEQIELL
                     NRRLSESVPCMPHKKAG"
     gene            207..908
                     /gene="mlrA"
                     /locus_tag="OO130_002831"
     CDS             207..908
                     /gene="mlrA"
                     /locus_tag="OO130_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HCR5366163.1"
                     /translation="MGLPLIPVTLRAWQRRYGLLKPQRTDDGHRLFNDADIDRIREIK
                     RWIDNGVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRTWIKERGQDYP
                     AQTLTTHLFIPLRRRLQCQQPTLQALLAILDGVLINYIAICLASARRKQGKDALVVGW
                     NIQDTTRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGENRTSAQQQQL
                     TSWQEQGHDIFPLGI"
     gene            968..1075
                     /locus_tag="OO130_002832"
     CDS             968..1075
                     /locus_tag="OO130_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HCR5366164.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(1056..1787)
                     /gene="yehW"
                     /locus_tag="OO130_002833"
     CDS             complement(1056..1787)
                     /gene="yehW"
                     /locus_tag="OO130_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HCR5366165.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFVVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(1792..2718)
                     /gene="yehX"
                     /locus_tag="OO130_002834"
     CDS             complement(1792..2718)
                     /gene="yehX"
                     /locus_tag="OO130_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HCR5366166.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVRLLSLRSVADYVCREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLEEA"
     gene            complement(2711..3868)
                     /gene="yehY"
                     /locus_tag="OO130_002835"
     CDS             complement(2711..3868)
                     /gene="yehY"
                     /locus_tag="OO130_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HCR5366167.1"
                     /translation="MTYFRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVGCAWLTACFIPGKKGSICALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            complement(3875..4792)
                     /gene="osmF"
                     /locus_tag="OO130_002836"
     CDS             complement(3875..4792)
                     /gene="osmF"
                     /locus_tag="OO130_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HCR5366168.1"
                     /translation="MPLSKVWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAQ
                     QGYEKVKKLDSEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLKEGGTF
                     KLAASAEFIERADALPAFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQISGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWTK"
     gene            complement(5044..7341)
                     /gene="bglX"
                     /locus_tag="OO130_002837"
     CDS             complement(5044..7341)
                     /gene="bglX"
                     /locus_tag="OO130_002837"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HCR5366169.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPFTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSTMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMEELDDAARHVLNVKYDMGLFNDPYSHLGSKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNAV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDMVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            7537..9252
                     /gene="dld"
                     /locus_tag="OO130_002838"
     CDS             7537..9252
                     /gene="dld"
                     /locus_tag="OO130_002838"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCR5366170.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPSSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALQRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(9291..10223)
                     /gene="pbpG"
                     /locus_tag="OO130_002839"
     CDS             complement(9291..10223)
                     /gene="pbpG"
                     /locus_tag="OO130_002839"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HCR5366171.1"
                     /translation="MPKFRVSLFSLALMLAVPLAPQAVAKTAAAITASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(10397..10984)
                     /locus_tag="OO130_002840"
     CDS             complement(10397..10984)
                     /locus_tag="OO130_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HCR5366172.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            11154..11732
                     /locus_tag="OO130_002841"
     CDS             11154..11732
                     /locus_tag="OO130_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5366173.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDHQA"
     gene            complement(11862..12623)
                     /locus_tag="OO130_002842"
     CDS             complement(11862..12623)
                     /locus_tag="OO130_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR5366174.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLDLGKLPEGAQALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKAMALELVRHKILVNAVAPGAIATPMNGMDDSDVKPDAEPSIPLRRFGATHEIA
                     SLVAWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(12676..>13520)
                     /gene="mdtQ"
                     /locus_tag="OO130_002843"
     CDS             complement(12676..>13520)
                     /gene="mdtQ"
                     /locus_tag="OO130_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HCR5366175.1"
                     /translation="AALNTVLQQIEKEQNTIIATDRQLYQTGITSSVEGVETDINASK
                     TRQQLNDVAGKMKIIEARLSALTNNQTKSLKLKPVALPKVASQLPDELGYSLLARRAD
                     LQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQDALHLSDLFRHSAQQMGVTAGLTL
                     PIFDSGRLNANLDIAKAESNLSIASYNKAVVEAVNDVARAASQVQTLAEKNQHQAQIE
                     RDTLRVVGLAQARFNAGIIAGSRVSEARIPALRERANGLLLQGQWLDASIQLTGALGG
                     GYKR"
BASE COUNT         3242 a         3779 c         3411 g         3088 t
ORIGIN      
        1 tgctggtcac ttgccgcagg agctaatcct agttttatcg caagccagat gggccacaca
       61 aacgcacaaa tggtattcaa tgtttacgga gcatggatga aagacaacaa tcacgaacag
      121 atagaacttc ttaacagaag actatctgaa agtgtcccat gcatgcccca taagaaagcg
      181 gggtaaaata aaaacttgca aaatcaattg gtttaccttt aatccctgtc acgttacgcg
      241 cgtggcagag gcgttacggg ttgctgaaac cgcaacggac agacgacggt catcgactgt
      301 tcaacgatgc cgatattgac cggatccgcg agatcaaacg ctggatcgac aacggcgtgc
      361 aggtcagcaa agttaaaatg ctgctcagta atgaaaatgt tgatgtgcag aacggctggc
      421 gcgatcagca agaaacatta ctgacctacc tgcaaagcgg caatctgcat agcctgcgaa
      481 cgtggatcaa agagcgcggt caggattacc ccgcccagac actcaccaca catctgttta
      541 ttcctctgcg ccgacggctt cagtgccaac aaccgactct ccaggcgctg ctggcgatcc
      601 tcgacggcgt actgatcaac tacatcgcca tttgtctggc ttcggcacgt agaaaacagg
      661 gtaaagatgc gctggtggtt ggctggaata ttcaggatac cacccgtctg tggctggagg
      721 gctggattgc cagtcaacaa ggatggcgca ttgatgtcct cgcccactcg ctcaatcaac
      781 tacgccctga actattcgaa ggccgtacat tgctggtgtg gtgcggtgaa aatcgaacct
      841 ccgcccaaca gcagcaactc accagttggc aagaacaagg ccatgatatt ttcccactcg
      901 gcatttaatg attcgttaac aaatgcgctt tactgtacaa tcctttcgtt aacataagga
      961 gtgcaatatg cgcatagcta aaattggggt catcgccctg ttcctgttta tggcgttagg
     1021 cggaattggt ggcgtcatgc tcgcaggtta tacctttatt ttgcgtgctg gctaagcgcc
     1081 tgcaccagcc tttcaaacag gcggtctgca atgatcgccg ccagtgccac cagtaacgcc
     1141 ccctggatca catacgcggt attaaatccg ctaagcccga tgatgatggg cgtacccagc
     1201 gtgctggccc ctaccgttga ggcgatcgtc gccgtaccaa tgttgataat caccgaagtt
     1261 cgcacgcccg ccagaatcac cggagccgcc agcggtagct cgaccttacg cagtcgctga
     1321 ccacgactca ttcccatacc tttcgcaact tctgtcacgc tggcatcaat cgctcccagc
     1381 ccggcaagtg tcgcctgcag gacgggcagc acaccgtaaa ggatcaaggc gataatcgct
     1441 ggttgcagac caaagccgat caccggaacg gcaatcgcca gcactgcgac aggcggaaaa
     1501 gtctgtccaa cggcggcaat agtttccacc agtgggcgaa attccgcgcc ccacgggcga
     1561 gtgacagcaa ttccggcacc agtgccaatg atcaccacaa acaaactcga aattcccacc
     1621 agccagaaat gagccagtgc cagagctgca aaactttctt gctgataaac gggtcgtggc
     1681 agttgtggga acaaggcagc aaacagcggc tggctgtaag gcagccagaa aatcagtgcc
     1741 acaaacaaag caatgagcca gaacagcgga tcgcgcaaca tcttcatacg cttacgcctc
     1801 ctccagcaga tcctgaaaat gcagcgtgcc gcaaggctgg ccctgcgtgt tcaccaccgg
     1861 cagcacctcg catccccgcg cgacaaacag ggagagcgca tcgcgtagcg tcatctcttc
     1921 tgccagtgcc tcaccttctg cccgttcttc gcgacacacg taatccgcca cactacgtaa
     1981 cgaaagcagg cgcacaccca gttcgctgcg cccaaaaaac tggcggacaa aatcgtttgc
     2041 cggacgagtc agcatcgtca gcggattgcc ctgctgcacc acttcaccgt gatccatcaa
     2101 taccagatgt tctgccagcc gtagcgcctc atcaatatca tgagtgacca gcacaatggt
     2161 acgccccagc aaacggtgaa tgcgcgtcat ctcttgttgc aacgcgccgc gcgttaccgg
     2221 gtccagtgcg ccaaaaggtt catccatcag taagacttgc ggatcggcag ccagtgcgcg
     2281 cgccactccc acacgttgct gctgaccacc ggaaagctga tgcggataac gctcacgtaa
     2341 atttgactcc agccccagta gcgccattaa ttcgtcgata cgatcgtcaa tccgcgcccg
     2401 cgaccatttt tgtaattgcg gcacggtagc gatgttttgc gccacgctcc agtgggggaa
     2461 caggccaata gattgaatag catagcccat ccggcggcgc aactccagta ctggcagcga
     2521 gcgaatttct tctccggcaa agcggatcac tccgctgtca tgctccacca ggcggttaat
     2581 cattttcagg gtggtggatt tgccggagcc agatgtgcca atcagcaccg aaaaactccc
     2641 ttcctgaaaa ttgagattga gatcgttaac ggctttttgt gcgccgaaca gtttgctgac
     2701 atggctaaat tcaatcatta cgtttcacct tcagcagtgc gataagcaaa tcgaacagcg
     2761 cgtcggtaag caccgccaga acaattaccg ggatcacccc cagcaacact aaatcaatgg
     2821 cgctgcttag cagcccctgg aaaaccagcg caccaaaacc gcctgcgccg attaacgccg
     2881 caatcaccgc catacctaca gtttgcacca tcaccacccg caggctgcgc agaaataccg
     2941 gtaacgccag tggtaactga acatgcagga atcgctgcgc cccgctcatg cccatcgctc
     3001 tggcactctc cagcacatcg cgcgggatct ggttcaaacc gactaccacg ccgcgcacca
     3061 gcggcagcaa ggcatagagc accagcgcaa tcagtgcggg tgtcattccg gttcctgcta
     3121 tgccgagctt ccccagccac ggaaaggccg tcaccagcgc ggcaagcggc gcaatcaaca
     3181 ggccaaagag cgccaccgaa ggcacggtct gaatgacatt gagcagagaa aaaattgccc
     3241 cctgccgagc agtggaaaag tagcaccaga tgcccaacgg cacaccaatc actaacgcag
     3301 gcagcaccgc accaaacagc aacgtcagat gttgtgccag cgcgtcgtca aacacatcct
     3361 gacggttggc gtattctttc attagtgaga gatcgttaag cgtgccggag tacagcaacc
     3421 acagcggaat aatggcaatc tgcatatgca acaaccagcg ccacagcgga tgcgtggaga
     3481 ttcggcggat ggcatcgcta caggccagca atgccagcgc cgcagccagc cagaaaccac
     3541 tgccgaggct ggtacgcgcc agcgcactgc cattttgcgc cagttgtgtc gccgcctttc
     3601 ccgcgcccca caccagcaat acgaagacga attgcgccag aatgagtgca caaatgctgc
     3661 cttttttacc gggaataaaa caggccgtca gccaggcgca accaacgccc accagcatcc
     3721 agatcgtttg cggccacagc tgccagagat gacgcccctc acccgaaact aaacgattag
     3781 gcgcgtaact gataaacggc agcgccgctg cgattgccgt cagcaacagc agcagcgcca
     3841 gaacaggatt aatacggaaa taagtcacgg gaaattactt cgtccaccct ttttgtttca
     3901 ggtagtcggc agccactttt ttggcatcca gtccttccac agcaatgctg gcattcagtt
     3961 gctgcaatgt ttttgcatcg aggctggcga agactggctg tagccactgt gccatttgcg
     4021 gatactcctt caacaccgac tcacgcacca ctggtgcagg cgcgtagata ggttgcacac
     4081 cttgcggatc gcttaaggtt tgcagcccca gcgccgctac cgggccgtca gtgccgtaag
     4141 ccattgcagc attaacgcca gagatttgct gggcagcggc tttgatcgtc accgccgtgt
     4201 cgccaccagc cagtgacagc aactgatcct gaccgagctt aaagccatag gctttttcaa
     4261 acgcgggtaa cgcatcggcg cgttcgataa actctgccga ggctgccagt ttgaaggtgc
     4321 cgccctcttt cagataacga ctcaggtcgg caagcgaagt gagtttattt ttctctgcca
     4381 catcctgacg cacggcgata gtccaggtgt tatttgcagg cgcgggcgtc agccagatta
     4441 acttgttgtg ctccgaatcg agctttttga ctttctcgta accttgctgc gcgtttttcc
     4501 acgctgcatc gttttcatct ttaaagaaga aagcgccatt gccggtatat tccggataga
     4561 tatccagttc accggaagta atcgccccgc gcaccacagg agtcgttccg agttgcactt
     4621 tattcaccgt cggtacgccg tggctttcca gcacctgcaa gatgatgttg ccgagtagcg
     4681 cgccttcggt atcgattttt gaaccgactt taacggggga agccgcctgt agcggcaggc
     4741 tcacggctgc caacataacc agtgaacctg cccagacctt tgagagtggc atgatgcttt
     4801 cctcattctt ttactgttgt tttcagcgaa ttaagagaaa agcatagttg ataatgacag
     4861 agttagcctg agcggaagga ttcagttgca gaatcagata aatacttaaa gggcatattt
     4921 tgcccggcgt ttcttgccgg atgcggcgta aacgccttat ccggcctaca aaggtcgaaa
     4981 attcagcaag ttaagatatg aacatcaggc aaagggcgca aacgtcgcgc ccttactaaa
     5041 gcattacagc aactcaaact cgcctttctt aacgcgtgcg gaatcagtgc cgataaagac
     5101 attgaacttg ccaggctcgg cgtcatattt catctgttga ttccagaact tcagcgcctc
     5161 gatatcaatc gggaagctga cagtctgagt ttcgcccggt ttcagggtga ttttctcaaa
     5221 gcctttcagc tgcttcactg gacggctcat ggaagccgtc acatcctgca agtacatctg
     5281 tactaccgtg gccccttcgc gtttaccggt gttcgtcacc tgcacgctgg ccgtcacttt
     5341 gccgtcacgc ttcatggtcg gcgcagaaag tttcacatca gagacggtga aagtggtata
     5401 gctcagacca tagccgaacg gataaagcgc cccgttagct tcatcaaaat aacgcgaagt
     5461 gtatttgttc ggcttgtcgg cattatacgg acgaccggtg ttcagatggc tgtagtacac
     5521 cgggatctgc ccgacagaac gcgggaagga catcggcagc ttgccggacg ggttgtaatc
     5581 gccaaacaac acatcggcaa ttgcattacc gccttcagtc cccgcaaacc aggtttccag
     5641 aatcgcatca gcctgctgat cttctttcac cagcgccagc ggacgcccgt tcatcagcac
     5701 cagcaccagc ggtttaccgg tggctttcag cgccgcaatc aagtcacgtt ggctttgcgg
     5761 aatagtgata tcggtacggc tggaggcctc gtgcgccatc ccctgtgctt caccgaccac
     5821 agccaccacc atatcagatt gtttcgccgt ctgcaccgct tcatcaatca tctcttgcgg
     5881 cgagcgcgga tcgactttga ccgcttcttc atactgattc aggaaatcga taatgccttt
     5941 gtcactggta acgttcgccc ctttggcata cagcacttta ccgttttcac cgacggcatt
     6001 tttaatcccg gtcagtacgg tcacggattg atcggcaaca ccggctgcgg accagctgcc
     6061 catcacgtcg cgtttactgt ccgccagtgg cccaaccacc gcaatggtgg ccgatttttt
     6121 cagcggtaac gtttcgagac ggtttttcag caacaccagg ctttcgcgcg ccacttcacg
     6181 tgcttcttta cggtgcaggc ggctttcggc attggtatcc accgggtcag actctttcga
     6241 ccccaaatgg ctgtatgggt cgttaaacaa tcccatatca tatttaacgt tcagtacatg
     6301 gcgggcagca tcgtccagct cttccatcgt cactttgccg gatttgatca acccaggcag
     6361 atacttcgag taatactcgt cgctcatact catgttgatt ccggatttca gcgccacgcg
     6421 caccgcatct tccgggtctg ccgccgtgcc atgtttaatc agctctttga ttgcaccgtg
     6481 atcggaaacg gtgatgcctt taaagcccca ctggtcgcgc agaacatctt tcagcagcca
     6541 ggaatcggag gtggctggcg tgccgttcag cgagttcagc gccaccatca ccgcgccgct
     6601 gcctgcgtcc agccccgctt tgtacggcgg catataatca ttaaacaggc gctgcggact
     6661 catatcgacg gtgttgtact ctttaccgcc ttctaccgcg ccgtatgcgg caaagtgttt
     6721 gacgctggtc atcaccgagt agcgatctgc cgggctttta ccctgcatcg cttccaccat
     6781 ggttttaccc attgttgagg tgagatacgt atcttcgcca aaaccttcgg aagcacgtcc
     6841 ccagcgcgga tcgcgcgaga catcgaccat cggtgcccag gtcatattca ggccatcatc
     6901 tgccgcttca taagcagaga cacgtccgac cgttttcact gcatcgaggt taaaagacga
     6961 ggccagaccg aggctaatcg ggaacaccgt gcgctgaccg tgcagcacgt cgtaagcaaa
     7021 gaaaagagga attttcaggc ggcttaattc catcacctga tcctgcatgg cgcggatatc
     7081 ctgacgggtt acggtgttga aaatcgcccc aacctgaccg tctttgatca tctcgcggat
     7141 cgcctctttc gggttatccg ggccgacgct gattaagcgc agctgaccaa ttttctcatc
     7201 aactgtcatt ttcttaagca attcggtgac gaacgcatcc cgcgcttcgg gcgtgaatgg
     7261 atggttgccg aataaatcat ccgccagtgc tggctgcagg gccagactca ccgcgattcc
     7321 tactgaacat agccatttca tatggatttt tcttcctctg ttgcccgacg aggcagcgaa
     7381 aatggtgcaa aaaccgtagt ttgccataag catgatggag agaaaaaaag aatgctcagt
     7441 ttattgtctg aattttcaaa atattcactc gctgaattgt catacaaggc gctattctag
     7501 tttgtgatat tttttcgcca ccacaaggag tggaaaatgt cttccatgac aacaactgat
     7561 aataaagcct ttttgaatga acttgcccgt ctggtcggtc attcacacct gctcaccgat
     7621 cccgcaaaaa cggcccgcta tcgcaagggc ttccgttctg gtcagggcga cgcgctggct
     7681 gtcgttttcc ctagctcact actggaattg tggcgggtgc tgaaagcctg tgtcaccgcc
     7741 gacaaaatta ttctgatgca ggctgccaat acaggcctga ccgaaggatc gacgccaaac
     7801 ggtaacgact atgatcgcga tatcgtgatc atcagcaccc tgcgtctcga caagctgcac
     7861 gttcttggca agggcgaaca ggtgctggcc tatccgggca ccacgctcta ttcgctggaa
     7921 aaagccctca aaccgctggg acgcgaaccg cactcagtga ttggatcatc gtgtataggc
     7981 gcatcggtca tcggcggtat ttgtaacaac tccggcggct cactggtgca acgtggcccg
     8041 gcgtataccg aaatgtcgtt attcgcgcgt ataaatgaag acggcaaact gacgctggtg
     8101 aaccatctgg ggattgatct gggcgaaacg ccggagcaga tccttagcaa gctggatgat
     8161 gatcgcatca aagatgacga tgtgcgtcac gatggtcgtc acgcccacga ttatgactat
     8221 gtccaccgcg ttcgtgatat tgaagccgac acgcccgcac gttataacgc cgatcctgat
     8281 cggttatttg aatcttctgg ttgcgccggg aagctggcgg tctttgcagt acgtctagat
     8341 accttcgaag cggaaaaaaa tcagcaggtg ttttatatcg gcaccaacca gccggaagtg
     8401 ctgaccgaaa tccgccgtca tattctggct aacttcgaaa atctgccggt tgccggggaa
     8461 tatatgcacc gggatatcta cgatattgcg gaaaaatacg gcaaagacac cttcctgatg
     8521 attgataagt taggcaccga caagatgccg ttcttcttta atctcaaggg acgcaccgat
     8581 gcgatgctgg agaaagtgaa attcttccgt ccgcatttta ctgaccgtgc gatgcaaaaa
     8641 ttcggtcacc tgttccccag ccatttaccg ccgcgcatga aaaactggcg cgataaatac
     8701 gagcatcatc tgctgttaaa aatggcgggc gatggcgtcg gtgaagccaa atcgtggctg
     8761 gtagattatt tcaaacaggc cgaaggcgat ttctttgtct gtacgccgga ggaaggcagc
     8821 aaagcgtttt tacaccgttt cgccgctgcg ggcgcagcaa ttcgttatca ggcggtgcat
     8881 tccgatgaag tcgaagacat tctggcgttg gatatcgctc tgcagcgtaa cgacaccgag
     8941 tggtatgagc atttaccgcc ggagatcgac agccagctgg tgcacaagct ctattacggc
     9001 cattttatgt gctatgtctt ccatcaggat tacatagtga aaaaaggcgt ggatgtgcat
     9061 gcgttgaaag aacagatgct ggaactgcta cagcagcgtg gcgcgcaata ccctgccgag
     9121 cataacgtcg gtcatttgta taaagcaccg gagacgttgc agaagtttta tcgcgagaac
     9181 gatccgacca acagtatgaa tccggggatc ggtaaaacca gtaagcggaa aaactggcag
     9241 gaagtggagt aaaaattacg gatggcatag tatcgccatc cgtcattcac ttaatcgttc
     9301 tgtgccgtct gccccgccgc cgccatttgg gcggcttttt gttttttata gctcaacgct
     9361 gctgctggaa caggcatcac tttaccggtt tcaatccagg tacgcaggcg gctggcatcg
     9421 gcaaaatggg tatatttgcc aaacgcatcc atcactacca gcgccaccgg tttattattg
     9481 ataaccgtac gcatcaccag acaatggccc gccgcattgg taaaaccggt tttggttaac
     9541 tgaatattcc agttatcgcg atacaccaga tggttagtat tgcggaacgg cagcgtatac
     9601 gtcgggttag agaaggttgc catatcttcc cgagtagtac ttaactgccc gatcaacgga
     9661 tattgtttgc tggcaatgag cagtttggtt aagtcacggg cagttgaaac gttatgcacc
     9721 gacaatccgg taggttcaac aaagcgcgtg ttgttcattc cgagcgattt cgctttcgca
     9781 ttcattgcct taataaaggc tttgtaacca ccgggataat ggtgtgcaag gcttgccgcc
     9841 gcgcggtttt ctgaagacat cagcgccagc aacagcatat ctttacggct gatttcgcta
     9901 ttcagtcgta cgcgcgaata gacccctttc atctccggcg tctggctgat atccactttt
     9961 agtttttcat ccagcggcag tcgtgcatcc agcacaacca tcgcggtcat taatttgctg
    10021 atagacgcaa tcggacgcac cagatccggg tggttcgagt agatcacttt gttggtattc
    10081 agatcaacaa tcatcgcgct accggaggca atttcaggtt gtgaagcggt gatagcggct
    10141 gccgttttcg caacggcctg cggtgccaaa ggcacagcca gcatcagggc caggctaaat
    10201 aaagaaactc gaaatttcgg catgatgagc attcagatag tggttcacgc gcacaggttg
    10261 cgcaccgccg gagtaaggat ttactgaggc tagcgacgcc atcataacga gcaaaaagtg
    10321 cgatcgtcaa aggagaatcg tgaggaaatg ctgcattgct gacatttacg ccagcaatgc
    10381 aacgtcaaag aactttctag aacaaacgat aaccgtagcc ccatagtata acggttaggg
    10441 cgagcagcac ttccagtacc agcacgccaa tcgccagcgt cgaactagag aagctaaggc
    10501 cttcctcttt gttgatattc aggaagctcg gaataccaag gtaaagcagg tagccggtgt
    10561 aaaacagcgc caccgtgccg accagcgcac acaaccagac cagtggataa agcgccacca
    10621 aaccgcttaa aaacagcgga gttgcaacgt agcccgcgaa gaccatgcag tgcgcaagtg
    10681 acggacgctg cggataatta cgcgccatcc accagatgac ccgccccatt accgcaaccc
    10741 cagccagcat aacgccataa aacaggacag ccagcgccag tccggtaaac caggataact
    10801 tcaggatagt gccatcgcca aaattccagc caatctgtgt agtgccaatg aaggcgcaaa
    10861 tcaccggaat cgccgccatc agcaaaacgt ggtgggtgta atggtgagaa atcgtttcgt
    10921 tttcgcgatt aatcacctgc atttcacgat cgggatggga aaacagtccc cagacatggc
    10981 tcataccgcc cccttgttgt gagttcatga acctgacagt tcaagtataa gtcagcttgt
    11041 gattattttt tgttcgctca tgtaatttgc accgtctgat gtacgtcctt tttcagcgtg
    11101 aatgatttcc agggtgtatg cttacaggca accaaaggga gacagactgg cctatggatc
    11161 tcaatacact catctcacaa tatggttatg ccgcgctggt gatcggtagc ctggcggaag
    11221 gtgaaaccgt gactttgctg ggaggcgttg cggcacatca ggggctatta aagttcccgc
    11281 tggtggtact ttctgtggcg cttggcggca tgattggcga ccaggtgctt tatctgtgcg
    11341 ggcggcggtt tggcggcaag ctgttacgcc gtttctcgaa acatcaggat aaaattgagc
    11401 gggcgcagaa acttatccaa cgccatccgt atctgtttgt cattggtacg cgctttatgt
    11461 atggctttcg ggtgattggc ccgacgctga ttggtgccag ccagctgccg ccgaaaatct
    11521 ttctgccgct gaatattctc ggcgcatttg cctgggcgtt gatttttacc actattggtt
    11581 acgctggtgg tcaggtgatt gcgccgtggt tgcacaatct cgaccagcat ttgaagcact
    11641 gggtctggtt gattctggtc gtggttctgg tggtgggcgt gcgctggtgg ctgaaacggc
    11701 gcgggaagaa aaagccggat catcaggcgt aaaaccattg ccctgggtaa ggcgttcgcg
    11761 ccgcatccga catccggtgc accatgcctg atgcgacgct tgtgcgtctt atcatgccta
    11821 ccaaaatttt acccattgaa ataaccacgc acctcactgc gctattctgg gttgaactgc
    11881 ggattcgcca acataaagcc gccatccact atcaacgact gcccggtggt gtaatttgcg
    11941 ccctctgagc acagccacgc taccaggctg gcaatctcat gcgttgcgcc aaaacgccgc
    12001 agaggaatcg aaggctccgc gtcgggcttc acgtcgctgt catccatgcc attcattggc
    12061 gtggcgatcg ccccaggcgc gactgcgttc accaaaatct tatgcctgac cagctccagc
    12121 gccatcgctt tggttaaccc accgagcgca tgtttagcgg ctgtgtaggc gctggcatcc
    12181 ggcagcggcg tatgttcatg taccgatgta atgttgatga tgcgaccgcc ctgcccttgt
    12241 ttcaccatct ggcgggccgc aatttgtgag cataaaaatg caccatcgac atcaacggta
    12301 aaaatcttgc gccactcatc gaaagccata tcaagaaacg gcgctttggt cattgcgccc
    12361 gcattattca ccagcacatc aatacgcccc agccgttgaa tgagtttctc cagcgcctgc
    12421 gcaccttctg gcagtttacc gagatccagc tgcacgatct ccgcacgtac gccgtggcta
    12481 actacctcac gcgcggtatc ttttgcccct tcttcatctg agtgccaggt aatgccaata
    12541 tcaaaccctt gctgcgccag taataacgcg cactctttgc cgatccccga atcggaggcg
    12601 gtaataatcg caacctgtgc catcgagttc tccacttaac gctgaataaa cgttaagtat
    12661 agaaggcgca tatcatcagc gtttgtaacc cccgcccaac gcaccagtga gttgaatgga
    12721 ggcatccagc cactgccctt gcaataacag gccattggcc cgctcacgca gcgcggggat
    12781 tctggcttcg ctgacgcggg aaccagcaat gatgcccgcg ttaaagcgcg cctgcgcaag
    12841 acctaccaca cgcaaggtat cgcgctcaat ttgcgcctga tgctggtttt tctccgcaag
    12901 tgtctgaacc tgactggctg cccgcgccac gtcattcacc gcttcaacca ccgctttgtt
    12961 gtagctggcg atagacaagt tgctttcggc ttttgcgata tcgagattgg cgttaagacg
    13021 accactatcg aaaatgggta gcgtcaggcc tgccgtaacg cccatttgct gcgcggaatg
    13081 acggaacaga tcgcttaagt gcaacgcatc ctgttgcagg aaggccatca ggttgatgtc
    13141 aggataaaat gccgcttttg ccgcatcaat ggtgcttagc gatgactcaa cgtaccagtg
    13201 cgccgcctgc aaatctgccc gccgggccag taaggagtac cccagttcat caggaagctg
    13261 gcttgccact ttcggcaacg cgaccggttt aagcttcaat gactttgtct ggttatttgt
    13321 aagtgcgctt aaccgtgcct caataatttt cattttcccc gcgacatcgt tgagctgctg
    13381 ccgggttttg ctggcattaa tatcggtttc cacaccttca actgaagaag taatcccggt
    13441 ctgatatagc tggcgatcgg tcgcgataat ggtgttctgc tctttttcta tttgctgcaa
    13501 gaccgtgttt aacgccgcct
//
LOCUS       DAJTMC010000109        13479 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.109, whole genome shotgun sequence.
ACCESSION   DAJTMC010000109 DAJTMC010000000
VERSION     DAJTMC010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13479
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.109"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(102..3323)
                     /gene="carB"
                     /locus_tag="OO130_002844"
     CDS             complement(102..3323)
                     /gene="carB"
                     /locus_tag="OO130_002844"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HCR5366176.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     ERLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(3341..4489)
                     /gene="carA"
                     /locus_tag="OO130_002845"
     CDS             complement(3341..4489)
                     /gene="carA"
                     /locus_tag="OO130_002845"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HCR5366177.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
     gene            complement(4944..5765)
                     /gene="dapB"
                     /locus_tag="OO130_002846"
     CDS             complement(4944..5765)
                     /gene="dapB"
                     /locus_tag="OO130_002846"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HCR5366178.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAVKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSRESHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNSL"
     gene            complement(5932..6846)
                     /gene="rihC"
                     /locus_tag="OO130_002847"
     CDS             complement(5932..6846)
                     /gene="rihC"
                     /locus_tag="OO130_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HCR5366179.1"
                     /translation="MHLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMSGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPELFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVKVALDLDVKGFQQWVAEVLALAS"
     gene            complement(6912..7862)
                     /gene="ispH"
                     /locus_tag="OO130_002848"
                     /gene_synonym="lytB"
     CDS             complement(6912..7862)
                     /gene="ispH"
                     /locus_tag="OO130_002848"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HCR5366180.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            complement(7864..8313)
                     /gene="fkpB"
                     /locus_tag="OO130_002849"
     CDS             complement(7864..8313)
                     /gene="fkpB"
                     /locus_tag="OO130_002849"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR5366181.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            complement(8382..8876)
                     /gene="lspA"
                     /locus_tag="OO130_002850"
     CDS             complement(8382..8876)
                     /gene="lspA"
                     /locus_tag="OO130_002850"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HCR5366182.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSR
                     AKKQ"
     gene            complement(8876..11692)
                     /gene="ileS"
                     /locus_tag="OO130_002851"
     CDS             complement(8876..11692)
                     /gene="ileS"
                     /locus_tag="OO130_002851"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HCR5366183.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFAVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYHDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            complement(11735..12676)
                     /gene="ribF"
                     /locus_tag="OO130_002852"
     CDS             complement(11735..12676)
                     /gene="ribF"
                     /locus_tag="OO130_002852"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HCR5366184.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYVVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            complement(12684..12902)
                     /locus_tag="OO130_002853"
     CDS             complement(12684..12902)
                     /locus_tag="OO130_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HCR5366185.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGHFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            13005..13268
                     /gene="rpsT"
                     /locus_tag="OO130_002854"
     CDS             13005..13268
                     /gene="rpsT"
                     /locus_tag="OO130_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HCR5366186.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
BASE COUNT         3148 a         3805 c         3398 g         3128 t
ORIGIN      
        1 aaaagattta cttaatgggc aaaaaaaacg gtcctcatca gagaaccgta ttacttattc
       61 gatcaaatta gcggatgaaa aatatccgcc atgacacgct attatttgat ctgcgcgtgc
      121 atttcctgca ccgaaattac tttttcggtc gcgtcggcat tcagcgccat cgcggtagcg
      181 aaaccaccgt tcagggtggt gtcataatgc actttatatt gcagcgcact gcgacggatc
      241 acgcgggagt cttcaatcgc gcgacggcct gaagtggtgt tgatgatgta ggtatattcg
      301 ccattcttga tgcggtcctg aatgtgcgga cggccttcat gcaccttgtt caccagacgc
      361 ggattaatgc ccgcttcgcc cagcacaatc gccgtgccgt gggtcgcatc cagctcgaag
      421 ccctgtttca gcagttttgc cgccaggtcc accacgcgtt ctttatcgcc ttcgcgcacg
      481 gaaagcaacg cacgaccgtg tttcttcatg gtggagttgc tgcccagctg cgctttggca
      541 aacgcttcag cgaaggtgcg gcccacgccc atcacttccc cggtggagcg catttctggc
      601 cctaacagcg ggtcaacacc cgggaattta ttgaacggca gcaccacttc tttcaccgag
      661 tagtacggcg ggataacttc tttggttacg ccctgctcag ccagcgattt gccagccatc
      721 acacgcgccg ccacttttgc cagcggtacg ccggtggctt tggagacgaa cggaacggta
      781 cgcgccgcac gcgggttaac ttcaatcagg tagacttcgt tgtttttcac cgcaaactgc
      841 acgttcatca gaccgcgcac ctgcaattcg aaggccagtt tctgcacctg ctggcgcatc
      901 acatcctgaa tttcctgact taaggtgtag gctggcagag aacatgcgga gtcaccggag
      961 tgcacgcccg cctgctcgat gtgctccatg atgccgccaa tcagcaccat ttcgccgtcg
     1021 cagatggcat ccacgtcaac ttctaccgcg tcatcaagga agtgatccag caacactggc
     1081 gcatcgttag acacgctgac cgccgtctgg aagtagcgac gcaggtcagc ttcgtcatag
     1141 acgatttcca tcgcccgacc gccgagaacg taagacggac gtaccaccag cgggtagcca
     1201 atctctttcg ccttctctac cgccatttca atagcggtaa cggtggcgtt cgccggttgt
     1261 ttcagtttca gacgctcaac cgcatgctgg aagcgttcac ggtcttctgc acggtcgata
     1321 gcatccgggc tggtgccgat aaccggtacg ccagcagctt ccagcgcgcg cgccagtttc
     1381 agcggggtct gaccgccgta ctgaacgata acgcctttcg gcttctcaat acgcacgatt
     1441 tccagcacat cttccagagt taccggctcg aagtagaggc gatcggaagt gtcgtagtcg
     1501 gtggagacgg tttccgggtt acagttaacc ataatggttt cgtaaccgtc ttcgcgcagc
     1561 gccagcgagg cgtgtacaca gcagtagtcg aattcgatac cctgaccgat acggttcggg
     1621 ccgccgccga gaaccatgat tttttcacgg tcggtagacg gattcgcttc gcactcttct
     1681 tcataagtgg agtacatgta agcggtgtcg gtggcgaact ctgccgcaca ggtatccacg
     1741 cgcttataaa ccgggtgcag gtcatactgg tcgcgcagct tgcggatttc cgcttcgcgc
     1801 acgcccgcca gttttgccag acgcgcatcg gcaaagcctt tgcgtttcag ctggcgcagg
     1861 aattcagcgt tcaggccagt gatgcccact tccgctactt tctcttccag acgcaccagt
     1921 tcttcaatct gtaccaggaa ccagcggtca atattggtca ggttgaacac gccgtccaca
     1981 gacaggcccg cacggaaggc atcggcgatg taccagatac gctctgcgcc agcatctttc
     2041 agttcgcgac ggattttggt taacgcttcc gggtcatcga ggctcacttt cgggtcgaat
     2101 ccagtcgcac cgacttccag gccgcgcagc gctttttgca gggattcctg ctgcgtgcga
     2161 ccaatcgcca tcacttcgcc aaccgatttc atctgagtgg tcagacggtc gttagcacca
     2221 gcgaattttt cgaagttgaa gcgaggaatt ttagtaacca catagtcgat ggacggctcg
     2281 aaggaggccg gagtacgtcc gccagtgatg tcgttcatca gttcgtcgag ggtgtaaccc
     2341 accgccagtt tcgccgccac tttagcaatc gggaaaccgg tcgctttcga cgccagcgcc
     2401 gaagaacggg acacgcgtgg gttcatttcg ataacaatca ggcgaccgtt tttcgggttc
     2461 accgcaaact ggacattgga accaccggtt tcaacaccga tttcacgcag caccgccatc
     2521 gaggcgttac gcatgatttg atattctttg tcggtcagcg tttgggctgg cgcgacagtg
     2581 atggagtcac cggtatggat gcccatcgca tcaaagtttt cgatagagca gacgatgatg
     2641 cagttgtcgt ttttatcacg caccacttcc atctcgtact ctttccagcc gatcagcgac
     2701 tcatcaatca gcagctcttt ggttggggag agatccagac cgcgggcgca aatttcttca
     2761 aactcttcgc ggttataagc gataccgccg ccgctaccgc ccatggtaaa ggatgggcga
     2821 ataatgcacg ggaagcccac gtcagcggca accgccagcg cttcttccat cgtgtgtgcg
     2881 ataccggaac gcgcggtttc cagaccaatt ttcttcatcg ctacgtcgaa acgacggcgg
     2941 tcttctgctt tatcaatcgc atcggcggtc gcaccaatca tagtcacgcc gaactcttcc
     3001 aacacgccct gacgctccag ctccagagcg cagttcagcg ccgtctgacc gcccatcgtt
     3061 ggcagcaccg cgtccgggcg ctctttttca ataatcttgc gtactacttc ccagtgaatc
     3121 ggctcgatgt aggtcgcatc ggccatttcc gggtcggtca tgatggtcgc cgggttggag
     3181 ttcaccagaa tgacgcggta accctcttcg cgcagggctt tacatgcttg cgcgccagag
     3241 tagtcaaact cacacgcctg accgataaca atcgggcccg cacccagaat caggatactt
     3301 tttatatctg tacgttttgg catggctctt ttactcctga ttacttagcg gttttacggt
     3361 actgctcaat taactcgata aagtggtcga acaacggcgc tgcgtcgtgt ggcccagggc
     3421 tggcttccgg gtgaccctgg aagctgaacg ccggtttatc agtgcgatga atgccctgta
     3481 acgtaccgtc gaacagggat ttatgcgtga cacgcaggtt tgcaggtaat gttgcttcat
     3541 ccaccgcaaa accgtggttc tgggcggtga tcatcaccac gtttttctcc acatctttaa
     3601 ccggatggtt gccgccgtgg tgaccaaatt tcattttgac agtcttcgca ccgctcgcca
     3661 gcgccagcag ctgatgaccg aggcagatgc cgaataccgg aatatcggtt tcgaggaatt
     3721 tctggatggc ggtaatggcg taatcgcacg gggccgggtc gccaggaccg ttggagagga
     3781 agatgccgtc tggattcatt ttcagcacat cttccgcaga agtttgcgcc ggaacgatgg
     3841 tcaggcgaca gcctctatcc accagcatcc gcaggatgtt gcgcttggca ccaaaatcat
     3901 aagccacgac gtggaacggc agctcgtctt cttttttcgc ttctggcagg ccgccggtca
     3961 acgtccagct cccttgtgtc cagctatagg cttctgcggt ggtcacttct tttgccagat
     4021 ccatgccatt cagacctggg aacgcgcggg ctttttctaa cgccaacgcc gcatccgggt
     4081 tatcgcctgc gataatgcag ccattctgtg cgcctttctc gcgcagtaaa cgcgtcagct
     4141 tacgggtatc gatatcggca atcgccacga tgttatggcg cttcaggtaa gaagagaggt
     4201 cttcggtatt acggaagttg ctggcaatca gcggcaggtc gcgaatcacc agaccttgtg
     4261 catgtacctg agaagattct tcatcggcgt cattagtgcc gacattgcca atatggggat
     4321 aagtaagagt aacgatttga cgagaatagg aaggatcagt gaggatttct tgataaccgg
     4381 tcattgaagt attgaaaacg acttccccaa ccgccgaacc tgttgcccct atggcccgac
     4441 cgtgaaactg ggttccgtct tccagaacca atagcgctga cttaatcaaa acaccctcca
     4501 gagaatattc actcacttta tttgcatatt aattcacaat gatgacgtaa atcaatgcaa
     4561 atctgcttac cgacgaattt ctggcaaacg gcggcattct ggagatatgg gcgctaaaag
     4621 tcaacttaat ggcgggattt tttatattat tttgtggctc atgcatgttt tacaggcatt
     4681 aagcgacaaa aagatcatat aacctgttct ggaaaacgct tgcacagcaa gaagattgca
     4741 tgaaataaaa gcgagcaggg aaaagtggac caaatggtca aattttacat taaaacaaca
     4801 aaaacagaag acaaaacatg taatttttgg cacttataga caatttaaaa acataacact
     4861 aaaaataaaa gggccaaaat taaagccctt tataatcaat aaattaaatg tgttattttt
     4921 gcaccataac aaatattttg tggttataaa ctattgagat caagcacatc tcgcatatca
     4981 aaaagaccac tttccttacc actcaaccac aaagccgatc ttaccgcgcc gttagcaaat
     5041 gtcatgcggc tggacgcctt atgggtgatc tccagacgct cgccaatatc ggcaaacatc
     5101 gcggtatgtt cgccaacgat gtcacctgca cgcacggtgg caaaaccaat ggtgccaggc
     5161 acacgttcac cggtgtggct ttcacgactg tagaccgcgc aatctttcag atctttatca
     5221 agggcgtggg cgatcgcctc tcccattgcc agtgcggtgc ctgacggcgc atcaacttta
     5281 tgtctatgat gtgcttcaat aatttcgata tcggtgtagt cacccatcac tttggctgct
     5341 ttctccagca gcttaagcat gacgttaacg ccaacgctaa aattggcggc aaagacaatc
     5401 gcaatatcgg cggcagcgtc acgaatcgcc tgtttaccgg cttcgtcaaa ccccgtagtg
     5461 ccgatcacca tccctttgcc atgctggcga caaaaagcga gatggttcag cgtaccttcc
     5521 ggacgggtaa aatctataaa cacatcaaaa tcatctttta ccgcatcgag gctgctttgc
     5581 acggtaacgc ctgttttccc tgcaccagcc agctcaccgg cgtcgctgcc cagtaaagaa
     5641 gatccttcgc gctccagcgc cgcgcccagc tgcacgcctt ctaatgccag cgccgcctga
     5701 atcaactggc ggcccatacg ccccccggct cccgcgatgg caacgcggat gtttgcatca
     5761 tgcatagcta ttctcttttg ttaatttgca tagaccgttt tcagagtaac cagcgcatcg
     5821 ctacgccgcc agccaaaaac gctgataatt agaaattaat gataaacagg gagggatatc
     5881 agtgaaaggc atgagtcgat gaatgactgc atgccgataa catgtgacag gttacgacgc
     5941 cagagccagc acctcagcca cccactgctg gaagcctttc acatccagat ccaatgccac
     6001 ctttacattg gctggtttgc ccaggcaacc gtcgatatca accaccgtcg tgcctgaggt
     6061 aaattcgccc tgagtttcca ctgccacaaa acacggtttg agagtgaaca attccgggcg
     6121 caccagccag gcaatagcgc aaagatcgtg catacgcagt ccactttgca tactgccgct
     6181 acggtagtgg ctaaacaggg cgtggagcat ttttccggtg cgattcaact ccggcaaagt
     6241 agcgagatag tcaggggtta atattgcctg attggtgaca tccagaccgc tcatgacgat
     6301 ttcgatgccg ctacggaaga cacaggcagc cgcttctgga tcggcagcga tattaaactc
     6361 ggcgtttggt gtgcagttgc cgcgaccagc agaaccgccc atgatcacca gacgacgaat
     6421 atacggttta cattccgggc attgtgaaag taacagcgca atattggtta acgggccgat
     6481 ggccaccagg gtaacgggct ctggtgcacg catcagggca tcccgaatcg ccagaaacgc
     6541 tggtatcccg agcggctttc ggttgtgctc aacaaagtcg tagccagcca ttcccgattc
     6601 gccgtgcaca gatgccgcat cacgcggtgc gcgtaccagc ggcacagcgg ctccctgggc
     6661 gagcggaatc tccgcattcc agaaatgcag cagttgcagg gcgttgcggg tggttttctc
     6721 aaccgagaca ttacccgcga cggtggtcat cagttgcagg tcgagttcgg gtgcaaaaat
     6781 cgcggcggca atggcgacgg catcgtcaat gccggggtca gtatcgagga agataggtaa
     6841 atgcatgttt tctccataaa aaatgccggt aacaagaccg gcattttcgc ataacttagg
     6901 ctgctaatga cttaatcgac ttcacgaata tcgacacgca gctctttcgg cacttcgaaa
     6961 acaatgtttt cttcacggcc ttccagcgga atggcttcac caccgcccag ctgctgcaaa
     7021 cgtgccacca cattctgcac cagaatatcc ggagccgatg cgcccgcagt cacgccgacg
     7081 catttaacct ctttcaccca ctcttcctgg atgtctttcg catcgtcaat caaaaacgcg
     7141 cgtttgccca tacgctgggc cagctccgcc agacggttgg agttggagga gtttttcgaa
     7201 ccgaccacca acacaacttc cgcctgttct gccagggcgc gtaccgcttc ctgacggtta
     7261 gtcgtggcgt agcagatgtc atctttgcgc ggaccgacaa ttttcgggaa gcgtttacgc
     7321 agcgcgtcga tcacatcaga cgtgtcatcc accgacagcg tggtctgggt cataaaggag
     7381 agcttctctt cgtttttgac cgtcagtttc cacacatcgt ccggcgattc gaccagatac
     7441 attccccctt ccgggttact gtactggccc attgtccctt ccacttccgg gtgcccggcg
     7501 tgaccgatga gaatagattc ttcgccacgg cgactggcgc gggcgacttc catatgcact
     7561 ttggtcacca gcggacaggt cgcgtcaaat accgtcaaat cgcgactttt tgcttcgtta
     7621 cgtaccgcct gagaaacacc gtgtgcggag aaaatcagga tcgcgccgtc cggtacttcg
     7681 ctaatctgct caataaagat agccccacgc tcacgcaagc tatcgaccac atagcggtta
     7741 tgtaccactt cgtgacggac atatatcggt gcgccgtaaa tggccagcgc gttttcaaca
     7801 atgctgatag cgcggtctac cccggcacaa aaaccacgcg ggttggccaa caggatctgc
     7861 atgttacgcc tccaatgccg gatcgatttc cagcacttca atatcaaaat gaacggtctg
     7921 cccggccaga ggatggttga aatcaacggt aatggagtcg ccgttaattt cgcggatcac
     7981 gccaggcatc tcactgccat ccattgcggt aaaaagcatg attgcgccaa tttctggctc
     8041 gcctgcatcc ataaattcac ggcgggagaa gtactgaatc aggtccggtg acggcacgcc
     8101 aaacgccgca tctggctcca acgagaaggt ggttttatcg cccactttca gccccaacag
     8161 gtgttgctcc agcccttcag aaagagaagc atcacccagg cggaacagcg ccggtttacc
     8221 gttgttgcgg gtagactcgg cggtggtgcc atcgtcgagt tttagcgtga agtgcaccag
     8281 gacggcgcta ttgctctgta cagattcaga catgcaggtt tgctcttata tttgaggatt
     8341 tttgccggat gcggcgtaaa cgccttatcc ggcagggttt attattgttt tttcgctcta
     8401 gaaggcaaaa aaccttccag cacaatcagt gccgcaccga cacagatggc agtatcggca
     8461 aggttgaagg tggcgaagtg ccagtcgccg acgtagaagt cgatcatatc gacaacgaag
     8521 ccgtgccaca ggcggtcgaa caggttgccc agcgcgccgc caataatcag cgcgtaagcg
     8581 atattgttta gcttctgcgt ggccttcgag cgatacatca tcactgccag gatcacgcta
     8641 ataccaatcg caataccggc aaagaaccaa cgctgccagc cgccgctatc ggcaaggaaa
     8701 ctaaacgccg cgccatagtt acgcgcataa tgcagattaa gcgacgggaa cagcgggacc
     8761 gtatccccca gagcaaagtt ctggaggatc aggtatttgc tgcccagatc gataatcagc
     8821 acgactacca ccagccacag ccagcgtagc cctgttgaac agatcgattg actcatcagg
     8881 caaacttacg tttttcaccg tcaccggcga cgttgctgac acagcggccg cagatttctg
     8941 cgtgttccgc caccttgccg acatcctggg tgtagtgcca gcagcgtggg cacttctcac
     9001 cttcggcttt actcaacgcg actttcagcc ctttgagtac ttcgctctgc tgagcatcag
     9061 caggtgcgtc gtgatagtct gcaacggtag cgccggaggt caacaggaca aatcgtaatt
     9121 catcgcccag cgcggtcagt ttcgccgcca gttccggttc tgcatacaag gttactgccg
     9181 cttccagcga gccacccact ttcttgtcgg cacgcgcttg ctcaatgact ttgttcactt
     9241 cgccacgcac tttcaacagc tcgtcccaga acgcatcgtt catcgcttcg ctgtctgcca
     9301 ggccaaacag gccttcgtac cactcaccgg tgaagacgta tttttcacgt tcgcccggca
     9361 ggtagcccca cacttcatca gcggtgaagg agaggattgg tgccatccag cgcaccagcg
     9421 cttctgcgat gtgatacagc gcagtctggc agctacgacg cgccacactg tccgctttgg
     9481 cggtgtactg acggtctttg atgatgtcga ggtagaagga acccatctca acggagcaga
     9541 agcgcatcag acgctgtacc acttcgtgga aatcgtatgc ttcgtacgcc ttgaggatgt
     9601 cttcctgtgc cgctttcgca caacctacgg cccagcgatc cagtaccacc atctcttccg
     9661 gtttcaccat atcttttgct ggatcaaaac cgttcaggtt tgccagcagg aagcgcgcgg
     9721 tgttacggat acgacgatag ctatcggcag cacgtttcag gatctcgtca gaaacggcca
     9781 tttcaccggt gtagtcggtt gatgccaccc acagacgcag aatatccgcg cccagtttgt
     9841 tcatcacatc ctgcggcgaa acggtattgc cgatggattt agacatcttg cggccctgac
     9901 catccacggt aaagccgtgg gtcagtacct gacgatacgg cgctttgcct ttcatcgcgg
     9961 tggagatcat tagggaagac atgaaccaac cacggtgctg gtcagaacct tccagataca
    10021 tgtccgctgc gtgaccggca aattccggac gcacgtcaac aacagaagag tgggtagatc
    10081 cggagtcaaa ccatacatcc aatgtgtccg gcactttcac gtactgatca gcttcatcgc
    10141 cgaggatctc tttcgcatca agatcccacc acgcctggat gccgtcaact tcaacgcgtt
    10201 ttgccacttc ttccattagt tcgagagtac gcggatgcag ctcttccgtg tctttgtgca
    10261 cgaacagtga catcggtacg ccccaggtgc gctgacggga gatacaccag tcaggacggt
    10321 tagcaaccat cgactcgata cgcgcctggc cccagtccgg gatccactgc acgcctttga
    10381 tctctttcag tgactgcgca cgcagacctt tctgatccat gctgacgaac cactgcggcg
    10441 tcgcgcggaa gatgatcggc gttttgtgac gccagcagca cggatagctg tgctgcattt
    10501 tctcaacgtg cagcagcgcg cctttttcct gcagcagcgc aacgacgatg tcgttcgctt
    10561 tgaagacgtt cacgccatcc agcgtcggat aagtgcccgg cagataagtg ccgtccgggc
    10621 caaccgggtt agcggtttcc aggccgtatt tctgaccgat cacatagtcg tccgggccgt
    10681 ggccaggcgc ggtgtgaacg gcaccggtac cggcatccag ggtaacgtga tcgccgagga
    10741 ttgccggaac gtcgaagccc ataaacggat gggtaaagcg cagcaactca agctccgcac
    10801 cttttaccgt gccgagaatg gtgtaatcgg tcacgccgat acgctgcatt acgctttcaa
    10861 ccagatcttt cgccagaatc acggcctgac cgtcgatctg caccagcgca tagtcgaaat
    10921 caggtgcaat agagattgcg cggttcgcag gcagagtcca cggcgtagtg gtccagatca
    10981 ccagcgagat tgggccgtta acgttgctta cggcaaattt tgctttcagt gcatcctgat
    11041 cgaccgcctg aaaagcaacg tcgatggacg gagaagtttt gtcgtaatac tcaacttccg
    11101 cttccgccag cgcagaacgg cagtcaacgc accagtgtac cggcttcgcg cctttgtgca
    11161 ggtgaccgtt gccgatgatt ttgcccagcg cgcggatgat gttggcttca gttttgaagt
    11221 ccatggtcag gtacgggtgc gaccagtcgc ccagcacgcc cagacggata aagtctttgc
    11281 gttgaccgtc aacctgggtc gccgcgtatt cgcggcactt ggcgcggaac tcggcggcgg
    11341 tgaatttctc acccggctta ccgtattctt gctctacttt cagctcgatc ggcagaccgt
    11401 ggcagtccca gccaggcaca tacggcgagt cataaccgga aagccctttg gacttcacga
    11461 taatgtcttt cagaatcttg ttaaccgagt gaccaatatg aatgctgcca ttcgcataag
    11521 gagggccatc atgcagaatg aaggtttttt tgcctttttt agccgcacgg atgatgccgt
    11581 acagatcatc atcagtccaa cgcgccagca ttcccggttc gcgcttggcg agatcgccac
    11641 gcatcgggaa ccctgtttcc ggcaaattca gggttgattt atagtcactc atcagattct
    11701 cggttccgtg tttcggtttg attacataac aggcttaagc cggttttgtt agcccaaaaa
    11761 attcgcgggc ggttaattca tcacgcgcaa tctgcgcttt cagttcgtcc agcgacgcaa
    11821 atcgctgctc attgcgtatt tttttacgca gcactacttg tatatggcga ccgtaaaggt
    11881 ccattgcaac atctaacaaa tgcacttcca gctgctggcg aataccggca accgttgggc
    11941 gtgttccgat gtttgccacg ccgggtaacg gcttttcacc gaggcccagc acttctacca
    12001 cataaacccc tttcaccggg gaaacctgac ggcgtagcgg tacattcgcc gtcgggaaac
    12061 ctatagtgcg ccctaattca tcaccgtgga ctacacgccc ggagatggca aacgggtgcc
    12121 ccagtaaact ttctgccaga gccagattgt catccgccag cgcctgacgc acagcggtgc
    12181 tgctaatacg cacgccacct tcgcaaaaag tttgcgtact agtgatatcg aagccgtatt
    12241 ccatgccagc tttctgtaat aacaagaaat cgccttcacg accagcgcca aagcggaaat
    12301 catcccctac ggcaagaaat tttacgcgca aatgcttcac cagaagatcg ctgatgaaat
    12361 tttgcgcggt taacgccgcg aaacgcctgt cgaaacgcac gcacagcacg taatcaacgc
    12421 cacactctgc aaggtaacgc agtttttccc gcagccgggt cagtcttgcc ggggctttat
    12481 cggtagcaaa cagttccagt ggttgaggtt caaaaagcat caccatcacc ggtaagttgc
    12541 gcttgcgccc ttcttcctgc aagccctgta acagcgcgcg atgaccgcga tgcacgccgt
    12601 cgaaattacc aatagtcagc acacaccctt cttgcggggc ctggctgaga ttatgtatgc
    12661 cgcgtatcag cttcatgtct ggctcaaaac agtgaaaatc gtccgagtat accttgtaca
    12721 gcggtcaagg ttaaccggcg attgagtacc gaaatcagaa agaagtggct tttcattgcc
    12781 atggcgcaaa tcacgggaag aaagtgaccg cctgctgcaa tttttatcgc ggaaaagctg
    12841 tattcacacc ccgcaagctg gtagaatcct gcgccatcac tacgtaacga gtgccggcac
    12901 attaacggcg cttatttgca caaatccatt gacaaaagaa ggctaaaagg gcatattcct
    12961 cggcctttga attgtccata tagaacacat ttgggagttg gaccttggct aatatcaaat
    13021 cagctaagaa gcgcgccatt cagtctgaaa aggctcgtaa gcacaacgca agccgtcgct
    13081 ctatgatgcg tactttcatc aagaaagtat acgcagctat cgaagctggc gacaaagctg
    13141 ctgcacagaa agcatttaac gaaatgcaac cgatcgtgga ccgtcaggct gctaaaggtc
    13201 tgatccacaa aaacaaagct gcacgtcata aggctaacct gactgcacag atcaacaaac
    13261 tggcttaatc gccaatttgc tgaagctttg tgaaaaagcc cgcgcaagcg ggttttttta
    13321 tgcctgctgc ttttatgatg cgtgagagaa cacactaccg gttgccagat agtcaaaaga
    13381 tgctggcttt tgcagacacg ccgttacact ttctgaactc ttcgtggctt aagaatcgta
    13441 gaaccatcgg ctgatacatt ctgaacaaga atatctact
//
LOCUS       DAJTMC010000110        13446 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.110, whole genome shotgun sequence.
ACCESSION   DAJTMC010000110 DAJTMC010000000
VERSION     DAJTMC010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13446
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.110"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            127..942
                     /gene="mepH"
                     /locus_tag="OO130_002855"
     CDS             127..942
                     /gene="mepH"
                     /locus_tag="OO130_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HCR5366187.1"
                     /translation="MAQINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNKTASA
                     SFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPYRWGG
                     SSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFFRTQG
                     RGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            1070..1651
                     /gene="sodB"
                     /locus_tag="OO130_002856"
     CDS             1070..1651
                     /gene="sodB"
                     /locus_tag="OO130_002856"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HCR5366188.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(1797..2966)
                     /locus_tag="OO130_002857"
     CDS             complement(1797..2966)
                     /locus_tag="OO130_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5366189.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVEPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGAGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYPGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAAAFAVVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(3132..3221)
                     /gene="ynhF"
                     /locus_tag="OO130_002858"
     CDS             complement(3132..3221)
                     /gene="ynhF"
                     /locus_tag="OO130_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HCR5366190.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            3520..4545
                     /gene="purR"
                     /locus_tag="OO130_002859"
     CDS             3520..4545
                     /gene="purR"
                     /locus_tag="OO130_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HCR5366191.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(4542..5474)
                     /locus_tag="OO130_002860"
     CDS             complement(4542..5474)
                     /locus_tag="OO130_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5366192.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     VVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            5587..6798
                     /locus_tag="OO130_002861"
     CDS             5587..6798
                     /locus_tag="OO130_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HCR5366193.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKAGNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEAGCQNH
                     GNAEVAHSESH"
     gene            7089..8237
                     /gene="cfa"
                     /locus_tag="OO130_002862"
     CDS             7089..8237
                     /gene="cfa"
                     /locus_tag="OO130_002862"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HCR5366194.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSMVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(8277..8918)
                     /gene="ribE"
                     /locus_tag="OO130_002863"
     CDS             complement(8277..8918)
                     /gene="ribE"
                     /locus_tag="OO130_002863"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HCR5366195.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            9133..10506
                     /gene="mdtK"
                     /locus_tag="OO130_002864"
     CDS             9133..10506
                     /gene="mdtK"
                     /locus_tag="OO130_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HCR5366196.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRLPSVI
                     ILQRASR"
     gene            complement(10547..11803)
                     /locus_tag="OO130_002865"
     CDS             complement(10547..11803)
                     /locus_tag="OO130_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HCR5366197.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQFTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPGGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGADLQQSTITVQQGGVLILDGSTVKGDGVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVTGDIHVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            12111..12187
                     /locus_tag="OO130_002866"
     tRNA            12111..12187
                     /locus_tag="OO130_002866"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12145..12147,aa:Val,seq:gac)
     gene            12192..12268
                     /locus_tag="OO130_002867"
     tRNA            12192..12268
                     /locus_tag="OO130_002867"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12226..12228,aa:Val,seq:gac)
     gene            12376..12681
                     /locus_tag="OO130_002868"
     CDS             12376..12681
                     /locus_tag="OO130_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HCR5366198.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            12807..>13446
                     /locus_tag="OO130_002869"
     CDS             12807..>13446
                     /locus_tag="OO130_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HCR5366199.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPINCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQ"
BASE COUNT         3316 a         3467 c         3203 g         3460 t
ORIGIN      
        1 tcgccaacga aacgtatttt ttaacaataa aagctattaa ctttctcttc ttctatgcat
       61 tagaatcatc aagttttgta aatcagacgc aggcatgata gacctgcctt aacagaggga
      121 cgctcagtgg cgcagataaa ccgtatttcg atcacgctct gtgctttact ttttaccacc
      181 ctgcctttaa cgcctatggc ccatgcttca aagcaagcca gggagagttc tgctaccact
      241 catatcacca aaaaagcaga taaaaagaaa agcacggcaa ccaccaaaaa gacccagaaa
      301 acagcgaaaa aagccgccag taaaagtacg accaaaagca aaaccgcttc ttccgttaaa
      361 aaatcttcca ttaccgcttc taaaaacgcc aaaactcgca gcaaacacgc cgtcaataaa
      421 acggcctcag ccagctttac cgaaaagtgt accaaacgta agggctataa atcgcattgt
      481 gtgaaagtca aaaatgccgc gtcaggaact cttgccgacg cgcacaaagc gaaggtgcaa
      541 aaagctacga aagtggcaat gaataaactg atgcagcaaa ttggtaagcc atatcgttgg
      601 ggtggcagct caccgcgtac cggttttgat tgcagcggcc tggtttatta cgcttataaa
      661 gatttggtga aaattcgtat tccgcgtacg gcgaatgaaa tgtatcacct gcgtgatgca
      721 gcgccaatcg aacgtagtga actgaaaaac ggcgacctgg tctttttccg tactcaggga
      781 cgcggcacag ccgatcatgt cggcgtgtat gtcggcaacg gcaaatttat tcagtcaccg
      841 cgcacaggtc aggaaattca aatcacctct ctcagtgaag actactggca gcgccactat
      901 gttggcgctc gtcgggtaat gaccccaaaa acacttcgct aaaactttac cctgttgtta
      961 cggcaacagg gtaagttcat cttttgtctc accttttaat ttgctaccct atccatacgc
     1021 acaataaggc tattgtacgt atgcaaatta ataataaagg agagtagcaa tgtcattcga
     1081 attacctgca ctaccatatg ctaaagatgc tctggcaccg cacatttctg cggaaactat
     1141 cgagtatcac tacggcaagc atcatcagac ttatgtcact aacctgaaca acctgattaa
     1201 aggtaccgcg tttgaaggta aatcactgga agagattatt cgcagctctg aaggtggcgt
     1261 attcaacaac gcagctcagg tctggaacca tactttctac tggaactgcc tggcaccgaa
     1321 cgccggtggc gaaccgactg gaaaagtcgc tgaagctatc gccgcatctt ttggcagctt
     1381 tgccgatttc aaagcgcagt ttactgatgc agcgatcaaa aactttggtt ctggctggac
     1441 ctggctggtg aaaaacagcg atggcaaact ggctatcgtt tcaacctcta acgcgggtac
     1501 tccgctgacc accgatgcga ctccgctgct gaccgttgat gtctgggaac acgcttatta
     1561 catcgactat cgcaatgcac gtcctggcta tctggagcac ttctgggcgc tggtgaactg
     1621 ggaattcgta gcgaaaaatc tcgctgcata ataactgatg gcaaatgcag cattgcctga
     1681 tgcgcttcgt ttaccatgcc aacgcggatg aggcgttaac agcgcattcg gcaagataaa
     1741 tcgcacgttg tcagcaactg taacgcagaa ggttatcctt ctgcgttttt gtttaattag
     1801 ctgttagcaa cgcaaactgt ttcaggttgt tttctggctg acataaacac cagcaataat
     1861 gccagtcccg cgacaatcgc tcccatcacc ggcacaaagc tgtatcccag cccagcggaa
     1921 attaccgcac caccagcagc tgctcccagc gcatttccaa gattaaaggc accaatattg
     1981 actgatgaag acagacctgg cgcttcactg gcgacacgca tcacgcgcat ctgtaacggc
     2041 ggtacgaccg caaaggctgc tgcgccccac accaccatgc taatagctgc gccgaactca
     2101 ttgcgggcca ggaacgggat tgccagcata atcaccatca acaacaacaa aaagcctttc
     2161 aacgtgccgt taactgaacg atctgccagt ttgccgccgg gatagttacc gatagagaat
     2221 ccgacaccaa tcagcaccag cattgccgtg acgaacaccg gtgttgcgtg ggtaatactt
     2281 tgcagtaccg gagagatata ggtgtagaga gtaaacattg caccagctcc cagtaccgtc
     2341 gtcagcaatg cagacaacac ctgcggacgc attaataccg ccagctcttt tttcacttca
     2401 ggtcgtgccc ctgcaccacc tttaggtaat gagaagaaca gacttaccat tgaaatcact
     2461 cccagccccg ccgttgccag aaatgacatc cgccagccga tggtttcacc caaccaggtc
     2521 gccgccggca cgccaccgat atttgccagg gttaacccca taaacatagt ggcaactgcg
     2581 ctggcctgtt tatgttttgg ctccacgctt gcggccacga ctgaacccaa accaaaaaat
     2641 gctccgtgat tcaggctggt caaaatgcgt gaaagcatca gggtcatata atccggcgcg
     2701 atggcggaaa gtacgttgcc gagcgtgaaa attgccatca ggaaaatcaa cgcactgcgg
     2761 cgggcacgat gagaaagtag aagcgtcatc agcggcgcgc caaccattac gccaactgca
     2821 taggcactga ttaacattcc ggcagcggga atcgagacat ccacaccgcg cgcaatgacg
     2881 ggcaacaagc ccattggcga gaactccgtt gtcccgatac caaacgcgcc aatcgccaga
     2941 gccagcaacg gatagttaat tttcatgcct tatctccacc tcttcgcgtc attacgcgat
     3001 attcattaaa gtggcgaaag catgacagca atcacaaaaa aatgaaaata acaaaaagag
     3061 aaaacacttt tgccattttg ctaacaaaca ggaaggagat gcgagggaga acgcgctccc
     3121 tcgagaggaa atcagtgcag cgcggcagtc aaacccacgg ccacgatcaa accgaggacg
     3181 ataatcgttg ttaccagtga aaatttaagg tcggtgctca tcaagttttc tcctttttta
     3241 ttaccacaca aatagtgata ttacgcattt ttacacactg tgatgaaaaa atctcccgtc
     3301 atttataatg ataagtgttt ttaccacttc cccttttcgg caagatcggc caaaattcca
     3361 cgcttacact atttgcgtac tggccattga ccccttcctg acgctccgtg tcgtttttcc
     3421 ggcgtaccgc aacacttttg ttgtgcgtaa ggcgtgtaaa ggcaaacgtt taccttgcga
     3481 ttttgcagga gctgaagtta gggtctggag tgaaatggaa tggcaacaat aaaagatgta
     3541 gcgaaacgag caaacgtttc cactacaact gtgtcacacg taatcaacaa aacacgtttc
     3601 gtcgctgaag aaacgcgcaa cgccgtgtgg gcagcgatta aagaattaca ctactcccct
     3661 agcgcggtgg cgcgtagcct gaaggttaac cacaccaagt ctatcggttt gctggcgacc
     3721 agcagcgaag cggcctattt tgccgagatc attgaagcgg ttgaaaaaaa ttgcttccag
     3781 aaaggttaca ccctgattct gggtaatgcg tggaacaatc ttgagaaaca gcgggcttat
     3841 ctgtcgatga tggcgcaaaa acgcgtcgat ggtctgctgg tgatgtgttc tgagtaccca
     3901 gagccgttgc tggcgatgct ggaagagtat cgccatatcc caatggtggt gatggactgg
     3961 ggtgaagcaa aagctgactt caccgatgcg gtcattgata acgcgttcga aggcggctac
     4021 atggccgggc gttatctgat tgaacgcggt caccgcgaaa tcggcgtcat ccccggcccg
     4081 ctggaacgta acaccggcgc aggccgcctt gccggtttta tgaaggcgat ggaagaagcg
     4141 atgatcaagg tgccggaaag ctggattgtt cagggtgact ttgaacccga atctggttat
     4201 cgcgccatgc agcaaatact gtcgcagccg catcgcccta ctgccgtctt ctgtggtggc
     4261 gatatcatgg caatgggcgc actttgtgct gctgatgaaa tgggtctgcg cgtcccgcag
     4321 gatgtttcgc tgatcggtta tgataacgtg cgcaacgccc gctattttac gccggcgctg
     4381 accacgatcc accagccaaa agattcgctg ggtgaaacag cgttcaacat gctgttggat
     4441 cgtatcgtca acaaacgtga agaaccgcag tccattgaag tgcatccgcg cttgattgaa
     4501 cgccgctccg tggctgacgg cccgttccgc gactatcgtc gttaatcacc cgttgcggga
     4561 gtctcttccg gctcccgcag ccactcctta ttcagcgtct cactatcgcc gagatactca
     4621 agcagccagg ttaacgcagg cgacatatca ttttgctgcc atgtcagaca acatgccgaa
     4681 tccgggaagg ggttttccaa ttcaagtgct acccacttcc cctcattaag ccacggtttg
     4741 gcgaaatgtg ttggcaccat ccctatgcat aatcctgccg agatacaggt tgccgatgat
     4801 tcccagtcag gcacgacaac tcttttttgg ttatccagta accaggtaat acgtttaggt
     4861 agcgttcgcg aggtgtcttc gcgcaccaac gacggccagt tgcgcaacgt atcatcgctg
     4921 aatgggccat ccatcaacgc cagcgggtgg tggctggcaa caacgcaact ccagcttagc
     4981 atccccatat cccggaaggc ataacgaccg cctaccggaa tcgcgcgtgt tgcgccaatc
     5041 gccagttcca cgcgcccgtc ggaaagcgca tcccagacac cgttgaacac ttcctgaaag
     5101 acaagaagtt cgacatcatc aaaatggcga taaaaatcaa cgatcatctg ccgtgtacgt
     5161 tctggcctga caatattatc cactgcgata gctaactgac cgcgccagcc gttcgctatc
     5221 tgctgacatt gctggcgggt gatctgcatt tttttgacaa cagagcgccc ttctttgaga
     5281 aaccacgctc cagcagcggt cagctccaca tcacggtgcc gtcgttcaaa gagcggcacc
     5341 gccagccact cttccagctg acgcacggta tagctgaccg cagaaggaac gcgatgcagc
     5401 tcctgtgccg cagcgctaaa actaccatta cgcgctaccg catcaacaac ttcgagtgaa
     5461 tattctgacc acatagtctg cctgcaaaat ttttgaaatc agtcatcaaa tattaccgtt
     5521 tcacaacact aatttcactc cctacacttt gcggcggtgt ttaattgaga gatttagaga
     5581 atatacatgc aacctgggaa aagattttta gtctggctgg cgggtttgag cgtactcggt
     5641 tttctggcaa ccgatatgta tctgcctgct ttcgccgcca tacaggccga cctgcaaacg
     5701 cctgcgtctg ctgtcagtgc cagccttagt ctgttccttg ccggttttgc cgcagcccag
     5761 cttctgtggg ggccactctc cgaccgttat ggtcgtaaac cggtgttatt aatcggcctg
     5821 acaatttttg cgttaggtag tctggggatg ctgtgggtag aaaacgccgc tacgctgctg
     5881 gtattgcgtt ttgtacaggc tgtgggtgtc tgcgccgcgg cggttatctg gcaagcgtta
     5941 gtgacggatt attatccttc acagaaagtt aaccgaattt ttgcgaccat catgccgctg
     6001 gtgggtctat ctccggccct ggctcctctg ttaggaagct ggctgctggt ccatttttcc
     6061 tggcaggcga ttttcgccac cctgtttgcc attaccgtgg tgctgattct gcctattttc
     6121 tggctcaaac ccacgacgaa ggccggtaac aatagtcagg atggtctgac ctttaccgac
     6181 ctgctacgtt ctaaaaccta tcgcggcaac gtgctgatat acgcggcctg ttcagccagt
     6241 ttttttgcat ggctgaccgg ttcaccgttc atccttagtg aaatgggcta cagcccggca
     6301 gttattggtt taagttatgt cccgcaaact atcgcgtttc tgattggtgg ttatggctgt
     6361 cgcgccgcgc tgcagaaatg gcaaggcaag cagttattac cgtggttgct ggtgctgttt
     6421 gctgtcagcg tcattgcgac ctgggctgcg ggcttcatta gccatgtgtc gctggtcgaa
     6481 atcctgatcc cattctgtgt gatggcgatt gccaatggcg cgatctaccc tattgttgtc
     6541 gcccaggcgc tgcgtccctt cccacacgca actggtcgcg ccgcagcgtt gcagaacact
     6601 ctacaactgg gtctgtgctt cctcgcaagt ctggtagttt cctggcttat tagtatcagc
     6661 acgccattgc tcaccaccac cagcgtgatg ttatcaacag tagtgctggt cgcgctgggt
     6721 tacatgatgc aacgttgtga agaagctggc tgccagaatc atggcaatgc cgaagtcgct
     6781 catagcgaat cacactgatc tatatcgata tacttatact taggctgcta acaaaatttt
     6841 gttgtatgat tgaaattagc ggcctatact aatttcgagt tgttaaagct acgataaata
     6901 ttatgttttt acggggacag gatcgttccc gactcactat ggatggtcat ttcggcaagg
     6961 gttcctcctt tccctctgtt ctacgtcgga ttatagactc gcggtttttt ctgcgagatt
     7021 tctcacaaag cccaaaaagc gtctacgctg ttttaaggtt ctgatcaccg accagtgatg
     7081 gagaaactat gagttcatcg tgtatagaag aagtcagcgt accggatgac aactggtacc
     7141 gtatcgccaa cgaattactt agccgtgccg gtatagccat taacggttct gccccggcgg
     7201 atattcgtgt gaaaaacccc gattttttta aacgcgttct gcaagaaggc tctttggggt
     7261 taggcgaaag ttatatggat ggctggtggg aatgtgaccg actggatatg ttttttagca
     7321 aagtcttacg cgcaggtctc gagaaccaac tcccccatca tttcaaagac acgctgcgta
     7381 ttgccggcgc tcgtctcttc aatctgcaga gtaaaaaacg tgcctggata gtcggcaaag
     7441 agcattacga tttgggtaat gacttgttca gccgcatgct tgatcccttc atgcaatatt
     7501 cctgtgctta ctggaaagat gccgataatc tggaatctgc ccagcaggcg aagctcaaaa
     7561 tgatttgtga aaaattgcag ttaaaaccag ggatgcgcgt actggatatt ggctgcggct
     7621 ggggcggact ggcacactac atggcatcta attatgacgt aagcatggtg ggcgtcacca
     7681 tttctgccga acagcaaaaa atggctcagg aacgctgtga aggcctggat gtcaccattt
     7741 tgctgcaaga ttatcgtgac ctgaacgacc agtttgatcg tattgtttct gtggggatgt
     7801 tcgagcacgt cggaccgaaa aattacgata cctattttgc ggtggtggat cgtaatttga
     7861 aaccggaagg catattcctg ctccatacta tcggttcgaa aaaaaccgat ctgaatgttg
     7921 atccctggat taataaatat atttttccga acggttgcct gccctctgta cgccagattg
     7981 ctcagtccag cgaaccccac tttgtgatgg aagactggca taacttcggt gctgattacg
     8041 atactacgtt gatggcgtgg tatgaacgat tcctcgccgc atggccagaa attgcggata
     8101 actacagtga acgctttaaa cggatgttta cctattatct gaatgcctgc gcaggtgctt
     8161 tccgcgcccg tgatattcag ctctggcagg tcgtgttctc acgcggtgtt gaaaacggcc
     8221 ttcgagtcgc tcgctaaagg ctattctatc gccccctctc cgggggcgat ttcagatcag
     8281 gcttctgtac ctggttgatt catggcattt tctcgtgccg ccagcacacg ttctaccgta
     8341 tctaccactg cctgagtttg tggatcaatt tcaatgttga cgcgtgcgcc gagttttttc
     8401 ttcccaagag tcgtgcgttc cagagtttcc ggaattaaat ggacgcaaaa acgcgttggc
     8461 gtgacttcgc cgacggtcag gctaataccg tcgatgccaa taaatccttt gtacaggata
     8521 tatttcatca actgactatc ctggacttta aaccagatct ggcgattatt ttccgaggtt
     8581 aatattttcg ccacttcagc ggtggtcata atatgacctg acattaagtg tccgccaatt
     8641 tcatcactga atttcgccgc acgctcaacg tttacccaat cccccacttt taaatcgcca
     8701 agattggtaa tgcgtaacgt ttctttcatc aggtcaaaac tgacatggtt gccgttaatt
     8761 tccgtcacgg tcaggcagca accgttatgc gccacggaag caccggtttc caggccgtcc
     8821 agcatgtggt cgggtaactc caccacatgc gtacgaaaat ttggtttctc gtcaatcgac
     8881 accagttttg cggtgccctg tacaatcccc gtaaacatac ttacaactcc tgaaatcagt
     8941 taagacattc tgttcagcac aatagcaggt ggcaaacgcc cttaccagtg aaggggtaag
     9001 aatgactatt ttttcactgg agaattaata aatcctcgct acaatagact gaatttcccc
     9061 tgcttcttct ttttgctgcc cattcaggcg gctttttagt ctctcatata actacaaata
     9121 aaaggtgttc acgtgcagaa gtatatcagt gaagcgcgtc tgttattagc attagcaatc
     9181 ccggtgattc tcgcgcaaat cgcccaaact gcgatgggtt ttgtcgatac cgtgatggcg
     9241 ggcggctata gtgccaccga catggcggcg gtcgctatcg gtacttctat ctggcttccg
     9301 gcgatcctct ttggtcacgg actgctgctg gcattaacgc cggttatcgc gcaattaaat
     9361 ggttccggtc gacgtgagcg cattgcgcat caggtgcgac aaggtttctg gctggcaggt
     9421 tttgtttccg ttctcattat gctggtgctg tggaatgcag gttacattat ccgctccatg
     9481 gaaaacatcg atccggctct ggcggacaaa gccgtgggtt atctgcgtgc gttgttgtgg
     9541 ggcgcgccgg gctatctgtt cttccaggtt gcccgtaacc aatgtgaagg tctggcaaaa
     9601 accaagccgg gtatggtaat gggctttatc ggcctgctgg tgaacattcc ggtgaactat
     9661 atctttattt atggtcattt cggtatgcct gagctcggtg gcgttggttg tggcgtggct
     9721 actgcggcgg tgtattgggt catgttcctt gccatggttt cttacattaa acgcgcccgc
     9781 tccatgcgcg atattcgtaa cgaaaaaggc accgcgaaac ccgatcctgc ggttatgaaa
     9841 cgactgattc aactcggttt accgattgcg ctggcactat tctttgaagt gacactgttt
     9901 gccgtcgtgg ctctgttagt gtctccgctc ggtattgttg atgtcgcagg acaccagatt
     9961 gccctgaact ttagttcact gatgtttgtg cttccaatgt cgctggcggc agcggtaact
    10021 atccgcgtag gttatcgtct gggtcagggc tcaacgctgg atgcgcaaac cgctgcgcgg
    10081 accgggctta tggtgggtgt ctgtatggca accctgacgg ccattttcac ggtttcactc
    10141 cgggagcaaa tcgccctgtt gtacaacgac aatcccgagg ttgtaacgct ggctgcgcat
    10201 ttgatgttgc tggcggcggt atatcagatt tctgactcaa tccaggtgat tggcagtggg
    10261 attttgcgtg gttataaaga tacgcgttcc attttctata tcacctttac agcttactgg
    10321 gtgctgggct tgccaagcgg ctatattctg gcactgaccg atctggtcgt tgaacctatg
    10381 gggccagcag gcttctggat aggctttatt atcggcctga catcggcagc cattatgatg
    10441 atgttgcgta tgcggttcct gcaacgtctg ccgtcagtta tcattctgca acgagcatcc
    10501 cgctaataaa gacaaggcgc aaccttcacg ggttgcgcct gtgtttttac gcaggctgga
    10561 gcgttgcgcc aatcccgtct tcgtctggct gtaatttcag agcgttgcac agagtttgcc
    10621 gactggcatc tgttatctca acgtgtatat caccggtaac ttcccctttc acattgatga
    10681 agtcaggtga gatttctttc gcactggttt gcaggcaaat cgctccctct ccgcgcaggc
    10741 gtttcacttt cagttgcaca tgcgttgccg caccagtgat cagccacagt tttccgccat
    10801 tcagattgat gttaccaaca ataaaagtga caccgtcacc ttttaccgta ctgccgtcga
    10861 gaatcaacac accgccctgc tgtacagtaa tcgttgattg ttgcagatct gcgccatagc
    10921 caataatcat tgaagcgccg tccagtaatg caacactccc ggtcagttca accggtgcgc
    10981 gatcttcctc tacgacaaaa ttttcgtccg cgctggtggg tgacaacagg atcactgttc
    11041 caccttgtat taccgcattt tccagcgtac cggcatgcac ttcagcccga ccacccacat
    11101 tcacggacag gttttgcgtc ttaccggaac tgtagagctg aaggccatcc gtccccagac
    11161 gataaatgga tccattctca acaatggtgt cagagtcttc accaccgaga ttattcatgg
    11221 tgccaccagc aagcaacgta tcactggctt tcccggccag cataaaaacg ccgccctgat
    11281 taatctcaca attgctggct tccccacctg gatacaccaa caattcacca ccttcatcta
    11341 ccacgaccgc tgaggttgtc ccattaacca acaggccgcc ctcttgatcg agaatgattt
    11401 tttccgcgcg atgtccttcc agtacacgca ggttaccgcc attttccagc aacaaaccgc
    11461 aggcataacc ctgatcaacg ctgaattcac cttcgggatg gcggccatta accgtagcga
    11521 gcgtagagag actaattgcg cctccctcgg actgaacaat atctgtggca atggcaccgt
    11581 cgctgaccga tagcgtgcca ttgttaccgt actgtgtgca tttggcgatc ccaccggttg
    11641 ccactttcaa ccagccagcc cccgtgacta cggtattttc ggctcttccg ccagcttcaa
    11701 caatcaactc gccttcagta aattgcgtcg cgccaatgac ctcgccggtc ttaacaattt
    11761 gcacattccc gtcgctcatc aagtttttcg catcagatcc catgatttat tcctttgctg
    11821 catctgtgtg cctttattgc tacctaaatg taaaggctac ggaggattta tccacgacag
    11881 atttgagatg gtggcaaaca actctgttta aactctgata cacgaattat tgggttgtat
    11941 cagatgtaaa tgcgatcctg aataaaaatc gcccttgcaa atcaacaaaa tatagccaat
    12001 ttgcttaaaa gctctgcaaa cggtcgaaat agcgcagaaa attacgtttt gcctcttgcc
    12061 accttcccac tctgccgcta atattcgtcc ccgttgtcac ctacaacgtt gcgttcatag
    12121 ctcagttggt tagagcacca ccttgacatg gtgggggtcg ttggttcgag tccaattgaa
    12181 cgcaccatcc tgcgtccgta gctcagttgg ttagagcacc accttgacat ggtgggggtc
    12241 ggtggttcga gtccactcgg acgcaccaga ttttcttaat ctggtcttct cctttttccc
    12301 tctgtttctt ctctgtatcc aatacgttaa aagatttaca ctgtcttcgt atgcgttatc
    12361 agaaggagaa tcgctatggc aactttgtta caacttcatt ttgcttttaa tggcccattt
    12421 ggcgacgcaa tggctgagca gcttaaaccg cttgctgagt cgattaatca ggaacctggt
    12481 tttctgtgga aggtatggac agaaagtgaa aagaaccacg aagccggtgg gatctatctt
    12541 tttactgatg aaaaaagcgc ccttgcctat ctggaaaaac atactgcccg acttaaaaat
    12601 ctcggcgttg aggaagttgt cgccaaagtt ttcgatgtca atgagccact gagtcaaatc
    12661 aatcaggcaa aactcgcctg acagaattta atcaagggcg gttagcgccc ttttcatccc
    12721 tgtctgaaat ttctcaaatt ctaaaaatct caaccaaact tatctgataa cactaaattc
    12781 gaaagaatgc gtacaggtaa gtaacaatga aaaaaattgc tattgtgggt gccgggccta
    12841 cggggatcta caccttattc tcgcttctac agcaacaaac tccactttct atttctatct
    12901 tcgagcaggc tgacgaggcc ggtgtcggga tgccatacag tgatgaggaa aactcaaaaa
    12961 tgatgctggc aaatattgcc agtattgaaa taccgccgat taattgtacg tatctcgaat
    13021 ggctacaaaa gcaagaagcc agccatctcc agcgttatgg cgttaaaaaa gaaaccttgc
    13081 acgatcgtca gtttttaccg cgaattctgc tgggcgaata tttccgcgat caatttttac
    13141 gattagtaga ccaggcacga cagcaaaaat ttgcagtggc tgtttatgaa tcatgccagg
    13201 ttaccgatct gcaaattaca aatgctggcg tcatgctcgc tacaaatcag gatttaccca
    13261 gcgagacgtt tgatttagcg gtgattgcca cgggtcacgt ctggcctgat gaagaagaag
    13321 caaccagaac gtattttccc agtccgtggt caggcctgat ggaagcaaag gtcgatgcgt
    13381 gtaacgtggg tattatggga acatccttga gcggactgga tgcggcaatg gcagtggcta
    13441 ttcagc
//
LOCUS       DAJTMC010000111        13369 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.111, whole genome shotgun sequence.
ACCESSION   DAJTMC010000111 DAJTMC010000000
VERSION     DAJTMC010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13369
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.111"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(171..1094)
                     /gene="dmlR"
                     /locus_tag="OO130_002870"
     CDS             complement(171..1094)
                     /gene="dmlR"
                     /locus_tag="OO130_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DmlR"
                     /protein_id="HCR5366200.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDVGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVS
                     RLATSAKVRITVEFLRQYFAEHYPNFSLEHA"
     gene            1197..2282
                     /gene="dmlA"
                     /locus_tag="OO130_002871"
     CDS             1197..2282
                     /gene="dmlA"
                     /locus_tag="OO130_002871"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HCR5366201.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFDLSFEQMG
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YINLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTYATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIA
                     HGPKTPDMKGNATTPQVADAICKIILR"
     gene            2532..4142
                     /gene="yeaV"
                     /locus_tag="OO130_002872"
     CDS             2532..4142
                     /gene="yeaV"
                     /locus_tag="OO130_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HCR5366202.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAAALSLFPQQSADAANAIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICAGLGSST
                     LYWGVAEWAYYYQTPGLNIAPRSQQALEFSVPYSFFHWGISAWATYTLASLIMAYHFH
                     VRKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSA
                     LTGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGAFLLPLLVLIVGPT
                     EFITNSIINAIGLTTQNFLQMSLFTDPLGDGSFTRNWTVFYWLWWISYTPGVAMFVTR
                     VSRGRKIKEVIWGLILGSTVGCWFFFGVMESYAIHQFINGVINVPQVLETLGGETAVQ
                     QVLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLQEGDDPDRGLRLFWC
                     VVITLIPLSILFTGASLETMKTTVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHQV
                     EHYLPQTPVEALEKTPVIPAGTVFKGDN"
     gene            4174..5298
                     /gene="yeaW"
                     /locus_tag="OO130_002873"
     CDS             4174..5298
                     /gene="yeaW"
                     /locus_tag="OO130_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaW"
                     /protein_id="HCR5366203.1"
                     /translation="MSNLSPDFVLPENFCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSSELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGEGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVHDLKLAARFTTRTSANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            5354..6319
                     /gene="yeaX"
                     /locus_tag="OO130_002874"
     CDS             5354..6319
                     /gene="yeaX"
                     /locus_tag="OO130_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaX"
                     /protein_id="HCR5366204.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHVI
                     VQMSDGDNQYSNAYSLLSSPHDTSCYQIAVRLEENSRGGSRFLHQQVKVGNRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDVDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALNEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(6373..7488)
                     /gene="rnd"
                     /locus_tag="OO130_002875"
     CDS             complement(6373..7488)
                     /gene="rnd"
                     /locus_tag="OO130_002875"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HCR5366205.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     SAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLTLVE
                     KAQTLPEEALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(7569..9254)
                     /gene="fadD"
                     /locus_tag="OO130_002876"
     CDS             complement(7569..9254)
                     /gene="fadD"
                     /locus_tag="OO130_002876"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HCR5366206.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGVGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAVVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(9459..10040)
                     /gene="yeaY"
                     /locus_tag="OO130_002877"
     CDS             complement(9459..10040)
                     /gene="yeaY"
                     /locus_tag="OO130_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HCR5366207.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGRSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            complement(10080..10775)
                     /gene="tsaB"
                     /locus_tag="OO130_002878"
     CDS             complement(10080..10775)
                     /gene="tsaB"
                     /locus_tag="OO130_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HCR5366208.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLAAIDARMGEVYWAEYQRDENGIWRGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPATEDMLPIACQIFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(10833..12743)
                     /locus_tag="OO130_002879"
     CDS             complement(10833..12743)
                     /locus_tag="OO130_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HCR5366209.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            12875..13219
                     /locus_tag="OO130_002880"
     CDS             12875..13219
                     /locus_tag="OO130_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCR5366210.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
BASE COUNT         3200 a         3555 c         3335 g         3279 t
ORIGIN      
        1 accagatagg tccagtaatt cagaaccccg tattcagcga acacattaac ctctttaatt
       61 atctttcgat catgcgcgat taaaggtgaa tatgctaacc aatctgtagc ggcttagaaa
      121 ggagaaaatc aggttttaac ccgatatcaa cccgataatt gaatcattaa tcaggcatgc
      181 tccagtgaaa aattcgggta gtgctcggca aaatactggc gtaaaaattc taccgttatc
      241 cgcactttcg ctgacgtcgc cagccttgaa acataaacgg cccagacgtt cgctggctgg
      301 taatattccg gtagcacttg cactaaatga ccactggcaa tgttttcgct aacatcccac
      361 caggaacgca gagcaatccc ctgcccatcg agacaccact gatggacaat ttctccatga
      421 ttagacgaca gtgggccggt gactttaatc gcatggtggc cttctttatt gcgtaattgc
      481 cagacaccaa aaggatgatc gcgctcttta atgaccaggc acggcaatgc agaaagatcg
      541 gttaaatgtt tcggcgcgcc atgttgcgca ataaattccg gcgacgcgca gagaatgcgg
      601 taattggtcg ccagtttgcg ggcgattaaa ttaggggcga tgtcatcgcc aatgcgaata
      661 tcgagatcga caccttcatt gaccaaatcg accagccggt cttccacatc aaaacgtaat
      721 tccagttgcg gatacgcctt cgccagcgct gataacgccg gagccaccac ctgtcgtcca
      781 aaaccaaagc tgctgataat acgcagcatc ccctgtggca cctgacgcac gtcagaaagt
      841 tcgtccatca tctgaccgac atcctgcaaa atccgctgcg cccattcata aatccgctct
      901 ccttcttcgg taatggtgac gcggcgggtg gtgcggtgta acaacaccac gtttagcgtt
      961 tgctccagca aggcgatgcg cttgctgacg aacgccggtg aaacgcccag ttcttccgcc
     1021 acggcggcaa aaccagcccg acgagccacc agcataaaga cgcgcaaatc attcagcagc
     1081 ggtaaattat tcatgattcg tgttttatgt ttcaccagtt acggggatta attccttttc
     1141 cgtcaattat aggatggtga tgttgtcaat tttgatggtc aggaagtgag aacccaatga
     1201 tgaaaacgat gcgtattgct gcgatcccgg gagacgggat tggtaaagaa gtccttcctg
     1261 aagggattcg cgtgttacag gctgccgctg agcgctgggg cttcgacttg agttttgagc
     1321 aaatggggtg ggcgagctgc gagtattaca gccatcacgg taaaatgatg ccggacgact
     1381 ggcatgagca acttagccgt ttcgacgcca tctattttgg tgccgtcggc tggccggata
     1441 ccgttccgga ccatatttcg ttgtggggtt cgctgctgaa atttcgtcgt gaattcgacc
     1501 agtacatcaa cctgcgcccg gttcgtctct ttcctggcgt tccctgcccg ctggcgggaa
     1561 aacagcctgg cgacatcgat ttttacgtgg tcagggaaaa caccgaaggc gaatattcct
     1621 cgctcggcgg tagagtgaat gaaggtacag agcatgaagt cgtcattcag gaatcggtat
     1681 ttacacgtcg tggtgtcgat cgcattttgc gttatgcctt cgaacttgcg caaagccgcc
     1741 cacgtaagac gctaacttat gccactaaat cgaacggttt agccatcagc atgccgtact
     1801 gggatgagcg agtggaagca atggccgaga attacccgga gatccgctgg gacaagcagc
     1861 atattgatat tctctgcgcg cgttttgtga tgcagccgga acgcttcgat gtggtggtgg
     1921 cgtccaattt gtttggcgat atcctttccg atcttggccc ggcctgcacc ggcaccattg
     1981 gcattgcccc atccgccaac ctgaatccgg aacgcacttt cccgtcgctc ttcgagcctg
     2041 tccacggttc cgcgccggat atctacggga aaaatattgc taaccctatc gccacgattt
     2101 gggccggggc aatgatgctc gattttctcg gcaatggcga tgagcgtttc cagcaagcgc
     2161 ataacggtat tctggcagca attgaagaag tgattgctca cgggccgaaa acacctgata
     2221 tgaaaggcaa tgccaccacg ccacaggttg ccgacgcgat ttgcaaaatt attttgcgtt
     2281 aaggtcaaac cagtttattt gaaccgcgtc actgacgcgg ttttttattc gttctttgca
     2341 gtaaataacc tgcgtcattt caccttttat tgtttccgtt tcgtgtttta tggctttccg
     2401 cattcttaat tgtttaattt atgtaatatt caaataatca atttgttaaa cacaacatac
     2461 aactcattac cctgcaaata attacaactg gcaaaagcca gagacgggct tttatgccca
     2521 ggagatgact catgatgagc aatgttaaga aaaaagatgt gccgctgata agcatcagcc
     2581 tggtggccat tcttttcatc gcagctgcat taagcctttt cccacaacaa tcggccgacg
     2641 cggccaacgc aatatacact tttgttacgc gtacgttagg ttccgccgta caggtattgg
     2701 ttttgctggc aatgggactg gtgatttatt tagccaccag taaatacggc aatattcgtc
     2761 ttggcgaagg aaaaccggaa tacagcacgc tctcctggct gtttatgttt atttgtgccg
     2821 gtttaggttc ttctacgctt tattgggggg ttgctgaatg ggcctattat tatcagacac
     2881 ctggattaaa tatcgcaccg cgttcacaac aggcactcga atttagcgtt ccctactctt
     2941 tcttccactg gggcatcagc gcctgggcaa cttatacgct ggcctcatta atcatggctt
     3001 atcactttca tgtgcggaaa aacaaaggtc tgagcctttc cggcattatt gctgctatta
     3061 ccggcgttcg cccgcaaggc ccatggggaa aactggttga tttgatgttc ctgatcgcca
     3121 ctgtcggcgc actgactatt tcccttgttg tcaccgcagc aacctttact cgtggacttt
     3181 ccgcactgac aggtttaccc gacaacttca ccgtgcaggc atttgtgatc ctgctttctg
     3241 gcggcatttt ttgcctaagc tcgtggattg gtatcaacaa cggtttgcaa cgtctgagca
     3301 aaatggttgg ctggggcgcg ttcctgctgc cattactggt gctgattgtc ggcccaaccg
     3361 aatttattac caacagcatc atcaatgcca tcggcctgac cacgcaaaac ttcctgcaaa
     3421 tgagcttatt caccgatccg cttggcgatg gttcatttac ccgcaactgg accgttttct
     3481 actggctgtg gtggatctca tacacccctg gcgtagcaat gtttgtcacc cgcgtttccc
     3541 gcggtcgtaa gattaaagaa gttatctggg gactgatcct cggcagcacc gtcggttgct
     3601 ggttcttctt cggcgtaatg gaaagctatg ccattcatca gtttatcaat ggcgtaatca
     3661 acgtcccaca ggtgctggaa acactgggcg gcgaaacagc tgtacagcaa gttctgatgt
     3721 cgttgccagc cggtaaattg ttcctcgccg catacctggg cgtgatgatt attttccttg
     3781 cctcgcatat ggatgcagtg gcctacacca tggcggcgac cagtacgcgt aatctccagg
     3841 aaggtgacga tcctgaccgt gggctgcgtc ttttctggtg cgtggtgatc actctgatcc
     3901 cgctttccat cttgtttacc ggtgcttcgc tggaaacgat gaaaaccacc gtcgtgctca
     3961 cagcccttcc cttcctcgtc attttactgg tgaaagtcgg cggatttatt cgctggctga
     4021 aacaggatta cgccgacatt ccggctcatc aagttgaaca ttatctcccg cagacaccgg
     4081 ttgaagccct ggaaaaaaca ccagtgatcc ctgcgggaac cgtattcaaa ggcgacaact
     4141 gagcgccatc atcctaacga taaaggtatc cctatgagca atctgagccc tgactttgta
     4201 ctacccgaaa atttttgcgc taacccgcaa gaggcgtgga ccattcctgc ccgtttttat
     4261 accgatcaga acgcgtttga acacgaaaaa gagaacgtct tcgccaaaag ctggatttgc
     4321 gtcgctcaca gcagcgaact ggcgaatgcc aatgattatg tgacgcgtga gatcattggc
     4381 gaaagcatcg tgctggtacg cggtcgtgat aaggttttgc gcgcgttcta taacgtgtgt
     4441 ccgcaccgtg gtcatcagtt gttgagcggt gaaggaaaag caaaaaatgt gattacctgc
     4501 ccgtatcacg catgggcatt caaactcgat ggcaatctgg cccatgcacg taactgcgaa
     4561 aacgtcgcca atttcgatag cgacaaagcg caactggttc cggtgcgtct ggaagaatat
     4621 gccggattcg tcttcatcaa catggacccc aacgccacca gcgtagaaga tcagttaccc
     4681 ggtctgggcg cgaaagtgct ggaagcctgc ccggaagtcc acgatctgaa actggcggcc
     4741 cgctttacca cccgcacgtc tgccaactgg aagaacattg tcgataacta tctcgagtgc
     4801 tatcactgtg gtccggcgca tccaggtttc tccgactccg tacaggttga tcgttactgg
     4861 cacaccatgc acggtaactg gacgctgcaa tacggtttcg ccaaaccgtc cgaacagtcg
     4921 tttaaatttg aagagggtac ggatgcggca ttccacggtt tctggctgtg gccgtgcacg
     4981 atgctgaacg tcaccccgat caaagggatg atgacggtca tttatgaatt cccggtggat
     5041 tctgaaacta ccctgcaaaa ctacgatatt tacttcacca atgaagagtt aaccgacgag
     5101 caaaaatcgc tgattgagtg gtatcgcgat gtgttccgtc cggaagattt acgtctggtt
     5161 gaaagcgtac agaaagggct gaaatcgcgt ggctatcgtg gtcaggggcg catcatggcc
     5221 gacagtagcg gtagtggcat ttccgaacat ggtatcgccc atttccataa tctgctggcg
     5281 caggtgttta aggactaatg acatcggcgg cggtattttc cgctgctggg ctgatttttg
     5341 atggagtaca gcaatatcag actatcaaat gtttgaagta caggtgagcc aggttgaacc
     5401 ccttaccgaa caggtgaaac gcttcacgct ggtggcaacc gatggcaaac cgctgcctgc
     5461 gttcaccgga ggaagtcacg tcattgtgca gatgagcgat ggtgataacc agtacagcaa
     5521 tgcgtattca ctactgagtt cgccgcatga cacctcttgt tatcagattg ccgttcggct
     5581 ggaggaaaac tcgcgcggcg gttcccgctt tttgcatcag caggtaaaag tgggcaatcg
     5641 gttaacgatt tcaacgccta ataacctgtt tgcgctaatt ccctcagcca gaaagcatct
     5701 gtttatcgcg ggcggtattg gtatcacccc tttcctgtcg cacatggcag agctgcaaca
     5761 cagcgacgtc gactggcagc tacattactg ctcgcgaaat ccagaaagtt gcgcatttcg
     5821 tgatgagcta gtccagcatc cgcaggctga gaaagttcac ttgcatcact catcaaccgg
     5881 aacacgactg gaattagcgc gattattggc ggatatcgaa cctggcacac acgtttatac
     5941 ctgtggtccc gaggcgctaa atgaagcggt aagaagtgaa gctgcgcgtc tggacatcgc
     6001 cgccgatacg ctgcactttg agcaatttgc tatcgaagac aaaaccggcg atgcctttac
     6061 cctggtgctt gcccgttccg gaaaagagtt tgtggtgccg gaagagatga ctattttgca
     6121 ggttattgaa aataataaag ccgcgaaagt ggaatgttta tgtcgtgaag gggtatgcgg
     6181 aacctgcgaa acagcaatac tggaaggtga agctgaccat cgggatcagt attttagcga
     6241 tgaagagcgt gccagccagc aaagtatgtt gatctgttgt tcgcgtgcga agggtaaacg
     6301 cctggtgttg gatttgtagt ttgccaaagc cggatgtggc gctgagcgcg cccagtccgg
     6361 cttcggaaga ttttactgcg gatattcctg caataaattg tgtaatgctt ccgccatcag
     6421 ctcaccacgc cagccggaaa tcagctccgg caaattgttc tgcggtttca gtttccagtg
     6481 ccagttcagc agttggttga tttgccgacg cgatgccagc aattcggcgc tgatcttatg
     6541 cgtttcgctc acgtcagtaa tcagcgactt aatcgcttta aacgctttac gataaccagg
     6601 catgtccatc aggttaagca tcggctgcgg taaggcctct tccggcaatg tctgcgcttt
     6661 ttccaccagc gtcagcagcg ttttaccgtg aaagcggatt tcgctaccgg ataaacccag
     6721 gctgtccagt tcgcctaaac ttcccggcat ataacgcgcg accgaccaca aatgctcttc
     6781 acgcacgaca aagttcaccg ccagatcacg ctctcgcgcc ttgcgcagtc gccagtcggc
     6841 taacagttgc agacaggcca gttggcgtgt gcgtaactgc caggcattgg tgatatcacg
     6901 ccaggcatct tccggcgcaa cgacttcctg acgacgcatt tgcatcaggc ggcattcatc
     6961 cagcgccgca ggtagccagc cggaggcctc cgtttctacc ataagcttgg cggtgatcgg
     7021 taacagatac cagacatccg ccgctgcgta ttcacactga cgttcggtca gcggtctggc
     7081 cagccagtcg gtgcgcgatt cactcttgtc cagcgtaacg ccggaatact cttccaccat
     7141 ggaagcgaaa ccccatgaca tcgggcgtcc gcagaaggca gaaaggattt gcgtgtcaat
     7201 caagggttgt ggtaattcgc caaagacatt gaggaacact tccagatctt cactgcctgc
     7261 atggagaaat tttgtgatgg acggatcgcg caggatcgct ttcagcggtg accagtcggt
     7321 gatcccgaga ggatcaatta gcgccagatg ctcgccatcg aaaagttgaa tcaaccccag
     7381 ctgcgggtaa taagtgcgcg tacgaacaaa ttcagtatcc agggctatcg ccggaaaggc
     7441 acggacggct tcacacaaag aagccagcgc atcgtccgtg gtaatcattt ggtaattcaa
     7501 attgttttct ctttagtggg cgtcaaaaaa acgccggatt aaccggcgtc tgacgactga
     7561 cttaacgctc aggctttatt gtccactttg ccgcgcgctt cgtcacgtaa ttctcgtcgc
     7621 aaaatttttc cgacgttaga tttcggtaac tcatcacgaa actccaccag cttcggtact
     7681 ttgtatcccg tgagctgacg gcggcaaaaa gtcaccagtg actcttcggt aagcgatgga
     7741 tcttttttca ctacgaagat tttcaccgct tcaccactgg agccggaagg tacgccaaca
     7801 gccgcgactt cctgtacgcc aggatgctgc atgacgacat cttcaatctc gttgggatag
     7861 acgttaaaac cggaaaccag aatcatgtct tttttacgat cgacaatgcg caggaatcct
     7921 tcttcatcca ttaccgcgat gtcgccggtg tgtaaccagc catttttgat gatttcatcg
     7981 gtagcatcgg gacgctgcca gtaacccagc atcacctgcg gtcctttgac acaaagctca
     8041 cccggttgac ctggtggtac ttcattatca tcatcatcca ccagtttggc ttccgtcgac
     8101 ggcaccggca aaccgatgct accactatga taatcaatat catatgggtt aacgctgacc
     8161 agcggcgcac actcggtaag gccatagcct tccagcagat actgtccggt cagtttcacc
     8221 caacgctctg ccaccacttg ctgcactggc atcccaccgc ctgcggaaag atgcagactg
     8281 gagaaatcca gctgctggaa ctctttattg ttcagcaacg cattgaacaa ggtgttaacg
     8341 ccagtgatag cggtaaacgg atatttcgct aactctttta ccaaccctgg aatatcacgc
     8401 gggttagtga taagcaggtt ctgcccaccc agttcgataa acagcaggca gttaatggtc
     8461 agggcaaaaa tgtgatacag cggcagtgcc gttactacca gttctttacc tggatgcagc
     8521 aacggcccat aggtggcgtt gacctgttcc aggttcgcca gcatattgcg gtgagtcagc
     8581 atcgcgcctt tcgccacacc tgtggtgccg ccggtgtatt gcaggaaggc taaatcttcc
     8641 ggcaccagtt cgggtttgac gtactgcatc cggtagccgt tatgcagtgc gctacgaaat
     8701 gaaatggcat ctggcagatg gtatttcggc accaaacgct tgatgtattt aacaacgaaa
     8761 ttgactaccg tgccttttgc cgtagatagc tgatcgccca tacgggtcag aattacgtgc
     8821 tgaacggcgg ttttatcaac cactttttcc agtgtgtgag caaagttaga cacgataaca
     8881 accgccgatg cgccgctatc gttaagctga tgctcaagct cacgcggggt atacaacggg
     8941 ttaacgttta cgacgatcat cccggcacgc aaaatgccaa acagcgccac cggatattgc
     9001 aataaattag gcatcatcaa cgcaacgcga tcgcctttct tcagccccac cccttgttgc
     9061 aaataagcgg caaacgcgcg actgcgttct tccagcttgc ggaaggtcat cacctccccc
     9121 atattcacaa acgcaggctg atcggcgtag cgcgcgaccg actgctcaaa catatctacc
     9181 agagattgat aacggtcagg gttgatctcc gtcggaacgt ccgcgggata acggttaagc
     9241 caaaccttct tcaattcttc acctctaaaa tgcgtgttcg tcgtcatcgc aaccccaaat
     9301 gatatacatg ccgttaacat aatattaact catcatacca gcttgataat tacccaacga
     9361 aaaggttgcg aagcgcgtca ctatttattt ttatctttac cgtaagaatg cagaaacagc
     9421 ggaccagccg ctgtttcttt ttctttaaaa acaagcaatt attcagttac aactgtttgt
     9481 actctcgcgg gaccgggatt ataccagccc catccgccgt agccataggg ccagccacga
     9541 ccgccataaa accatggatc aatcggctga ggcggcataa tcacctgctg ggttaaatgc
     9601 caacgtttgt aacccgttac ttgcatcacc ataaatttat agggcgtatt gccgattttg
     9661 ccgtcaaccg caccagtgat tggcccgact accgtaacca gttgtccacg gaaatccacc
     9721 gggtccagaa aaccgttcac atcggcataa atgcgaccgc gagaaggttc tcccagcgtc
     9781 ggtctggctc cgctgtccag cggtacggta gcaatttcca ggcgggtttt cccttgctgg
     9841 ttttgtaccg caaccacttt gccaccaaag cgtgcctcct gaccaacgta cagctgcggc
     9901 gcactcatca cccgaactaa atcttgttgc ggcgtgggac tgcggccttt aatggcgtcc
     9961 ggcacagtga cacaaccgct cagcattagc gcaaacgtac ctgccagtat gcctttgata
    10021 acattctttt gaaccgccat gatgcgactc ctttttttct cagagcatac tcttaagatt
    10081 cattctttgc ccggaagttt cttccatgcg acgttgttac gtaaataaac cggttcggca
    10141 tgttccaccg ccaccgtttt accctcggca aatatctgac acgcaatcgg cagcatatct
    10201 tccgtcgctg gcagtaaaac ttctccgtcg cgcaaaacca gcccgctctc tttaccgaga
    10261 tccggccagg cttgccagcc cgtccctacc gttacccatt cgccggaaag ctgttgcatt
    10321 cgttcatgaa cgagttcggg tttgagtacg gcttcggttt cttcaccgcg ccagataccg
    10381 ttttcatcac gctgatattc ggcccagtaa acttcgccca tccgcgcatc aatggcagcc
    10441 agcacgcggg ttgcgccgtt tttgcgccac gccccttgcg ccattgtcat tagcgtggag
    10501 acgccaatca tcggcagctc tgcgccaagt gccagccctt gcgcaatgcc aataccaatg
    10561 cgcacgccgg taaagctacc ggggccgcgc ccgtaagcca gagcgttaat atcagtcagg
    10621 gaagttccgc tggtggtcag gatatcctgc accatcggta agattcgttg agtatgttca
    10681 cgagggcaaa gctcaaaatg agcgttgaca gtaccgtcgt tccacagggc gacagagcag
    10741 gcctctgtcg cggtatcgat agccagaatt cgcatgggtc ttcgtgctta gatcaataaa
    10801 aaggcgcgca tcataccata ctccgtaaca aattacctgg aggatggtat cgcaaggaaa
    10861 cgaaccgcac gggcaatgtc acgggtgcgc ggcgcgggcg gcagactggc gagaaacgtc
    10921 gcgccgtaag ggcgcatcac cagccgattg tcacaaatca ccaaaacccc gcgatcgtcg
    10981 gcgtcgcgaa tcagtcgccc taccccctgc ttgagagtaa tcaccgcatc cggcagttgt
    11041 acttcatcga acgggtcgcc accgcgcaaa cggcaatctt ccatgcgcgc ttttaacagc
    11101 gggtcatccg gtgaggtaaa cggcaacttg tcgataatca ccaatgacaa tgtatcgcca
    11161 cgcacgtcca ccccttccca gaaactactg gttgccacaa gaagcgcgtt accggcgctg
    11221 acaaattgct gcaacagttg ccctttgctg gtttccccct gcaacaaaac gggaagcgtc
    11281 atggtagcgc ggaactgctc ggccagatca cgcatcatgg cgtgcgaggt acaaagcata
    11341 aaacaacgac cgttgttagc ttcgatgatc ggtcgcagca ttgccgctaa ctggcgagca
    11401 gaacctggct ggttggtttg cggcagattg cgcggcacac agagtaacgc ctggcggctg
    11461 taatcaaacg ggcttggcaa tagcaacgac tccgcctgtt ctatcccaag ccgcgaggtg
    11521 aaatgatgca gatcgtcgtt caccgacagc gttgctgagg tgaagatcca gctaccaggc
    11581 ttttgcgcca ttaactcttt gaatttatcc gccacgctga gcggcgtgag agccagagta
    11641 aaatggcgcg aagtgcattc gtaccagtag ctgtagcccg gctgattgat ctcttttagc
    11701 cgcttcagcc gtgtgcgata caacgtagcg cgctcaaatg ccgcatccag caaggcggaa
    11761 cgccccagtg acagtttcgc cacgtcataa caaagttcca gggtgtcatc gagcagtaaa
    11821 aatgcccgct gaatttgcgg attagctaac agctcacgca ggttaccgcg ataacctggc
    11881 tcaccgagtt gcagacgaaa atcctgcgca ctctgggcaa gacgatcagc gcacttttgt
    11941 aactgctggg tgtcttttaa ttcggtgcgg taggcgatgg tgatgtcttt tgccaggtcg
    12001 agcagttgtc gactggagag tgactgacca aaatactggc tggcaatgtc cggtagctgg
    12061 tgggcttcgt cgaagatcat gacgtccgct tccgggatca gttcgccaaa tccactctct
    12121 ttaaccacca tatccgccag aaagagatga tggtttacca ccaccacatc ggcgtccatc
    12181 gcttttttac gtgctttgac cacaaagcaa tctttataca tcgggcagtc gctgccaaga
    12241 cagttgtcgt tggtgctggt aaccagcggc cacgcctgtg aatcttccgc cacgctgacg
    12301 caggtgctga tatcaccatc gactgtttga ttagaccagg agcgcagcag gatcacatcg
    12361 cttaagattt gtaccggcag atcgcccccc gccagcgcct gctgttcgag acgttcgagg
    12421 cagaggtagt ttgagcgccc tttcagcagc gccacgttgc ccgtatattt caatgccttt
    12481 gagactgttg gcaaatcgcg gctgtagagc tgatcctgca acgcttttga gccggtcgag
    12541 ataatgactt tctttttcgc ccgcagcgca ggagccaggt aagcgtaggt tttgcccgta
    12601 ccggttcctg cttccaccac cagcggctgg cctttttcta tcgcctgggt gacggctacc
    12661 gccatctgtc gctgtggttc tcgcggctta aagcctggta tcgctttcgc cagctgaccg
    12721 tctggtgcaa aatcgtccgt cacactaccc cctgttgatt tgaacgggga ttatgtcagg
    12781 atgagggcgc tttcgccagt tgaagtggtg acggcaacct cacattgtgg cagtctttgc
    12841 agcacgaaaa tggaagtata atgaggacaa aatgatgact atcgttcgta tcgatgctga
    12901 agcccgctgg tctgatgtag taatccacaa caacacgctc tactacactg gtgtaccgga
    12961 aaacctcgac gccgatgctt ttgagcaaac cgccaacacg ctggcgcaga ttgacgccgt
    13021 gctggaaaaa cagggcagca ataaatcgag cattctggat gccaccattt tcctggctga
    13081 taaaaacgac ttcgcggcga tgaataaagc gtgggatgcc tgggttgtgg cgggtcatgc
    13141 gccggtgcgc tgcacggtgc aagcggggct gatgaacccg aagtataaag ttgaaattaa
    13201 gattgtggct gcggtgtaag ctttatcgaa gcaaaataag tcagacgata atttatcgat
    13261 aatactggtc ggttttacat aaatcgacca gagattaaga tattactgtt gatgctcctg
    13321 ctaattcatt atttattttt ataatttcaa ttttagctat aaagctaac
//
LOCUS       DAJTMC010000112        13313 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.112, whole genome shotgun sequence.
ACCESSION   DAJTMC010000112 DAJTMC010000000
VERSION     DAJTMC010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13313
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.112"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            <1..309
                     /locus_tag="OO130_002881"
     CDS             <1..309
                     /locus_tag="OO130_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCR5366211.1"
                     /translation="VVLNGKINGNACIVVPLSTTRDHDKLNRGMHVEIASNVINGLQF
                     FDQQIRWAKADLVQQVSRNRLNRARTYRGYLNQCLPHELVADIQRAVIKSINAILLIN
                     "
     gene            508..789
                     /gene="ppiC"
                     /locus_tag="OO130_002882"
     CDS             508..789
                     /gene="ppiC"
                     /locus_tag="OO130_002882"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HCR5366212.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(876..2351)
                     /gene="ilvC"
                     /locus_tag="OO130_002883"
     CDS             complement(876..2351)
                     /gene="ilvC"
                     /locus_tag="OO130_002883"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HCR5366213.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRCHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            2501..3391
                     /gene="ilvY"
                     /locus_tag="OO130_002884"
     CDS             2501..3391
                     /gene="ilvY"
                     /locus_tag="OO130_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HCR5366214.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSSEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(3388..4932)
                     /gene="ilvA"
                     /locus_tag="OO130_002885"
     CDS             complement(3388..4932)
                     /gene="ilvA"
                     /locus_tag="OO130_002885"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HCR5366215.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRAVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(4935..6785)
                     /gene="ilvD"
                     /locus_tag="OO130_002886"
     CDS             complement(4935..6785)
                     /gene="ilvD"
                     /locus_tag="OO130_002886"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HCR5366216.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDTLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(6850..7779)
                     /gene="ilvE"
                     /locus_tag="OO130_002887"
     CDS             complement(6850..7779)
                     /gene="ilvE"
                     /locus_tag="OO130_002887"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HCR5366217.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(7799..8062)
                     /gene="ilvM"
                     /locus_tag="OO130_002888"
     CDS             complement(7799..8062)
                     /gene="ilvM"
                     /locus_tag="OO130_002888"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HCR5366218.1"
                     /translation="MMQYQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(8059..9705)
                     /locus_tag="OO130_002889"
     CDS             complement(8059..9705)
                     /locus_tag="OO130_002889"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HCR5366219.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLATTKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPELQQPLNINDWQQYC
                     AQLRDEHAWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(9708..9758)
                     /locus_tag="OO130_002890"
     CDS             complement(9708..9758)
                     /locus_tag="OO130_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5366220.1"
                     /translation="MNNSTKFCFSTFSTGN"
     gene            complement(9845..9943)
                     /gene="ilvL"
                     /locus_tag="OO130_002891"
     CDS             complement(9845..9943)
                     /gene="ilvL"
                     /locus_tag="OO130_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HCR5366221.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            10295..11815
                     /locus_tag="OO130_002892"
                     /pseudo
     CDS             10295..11815
                     /locus_tag="OO130_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
     gene            complement(11840..12178)
                     /gene="maoP"
                     /locus_tag="OO130_002893"
     CDS             complement(11840..12178)
                     /gene="maoP"
                     /locus_tag="OO130_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HCR5366222.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            12297..13136
                     /gene="hdfR"
                     /locus_tag="OO130_002894"
     CDS             12297..13136
                     /gene="hdfR"
                     /locus_tag="OO130_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HCR5366223.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(13232..13307)
                     /locus_tag="OO130_002895"
     tRNA            complement(13232..13307)
                     /locus_tag="OO130_002895"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13272..13274),aa:Trp,seq:cca)
BASE COUNT         3093 a         3600 c         3448 g         3172 t
ORIGIN      
        1 gtcgtgctca acggaaaaat taatggcaac gcatgtatcg tcgttccatt atcaaccact
       61 cgcgatcatg acaagttaaa tcgtggaatg catgttgaaa tagccagtaa tgttatcaat
      121 ggcctgcaat tctttgatca gcagattcgt tgggccaaag ccgacctggt acaacaagtc
      181 agtcgaaacc gattaaatag agcaaggact tacagagggt atcttaatca atgtttgcca
      241 catgagttgg ttgcagacat acagagggcg gtcatcaagt caatcaatgc catattgctc
      301 ataaattgac aataaaataa ccatccccga taatgacttt gtagcccata gaggccctaa
      361 gttttctccc cttgagggac ctgcgaagcc cggattttcc gggcttttgc catatatgta
      421 ccagcccctc cctgactgac aattacgtta gcatctgcgg atcatgctaa aatcgccgcc
      481 tgacaattat tcactcaagg cacgatcatg gcaaaaacag cagcagcact gcatatcctt
      541 gtaaaagaag agaaactggc tctggatctt ctcgagcaga ttaagaacgg tgccgatttc
      601 ggcaagctgg cgaagaaaca ctccatttgc ccatcaggca aacgcggcgg tgatttaggt
      661 gaattccgcc agggtcagat ggttccggcg ttcgataaag ttgtgttctc ttgtccggta
      721 ctggagccga ccggcccgct gcacacccag ttcggttacc acatcattaa ggtgctgtac
      781 cgtaactaat agcaaggcct tctccaggag aaggccttga gtgttttctc cctctccctg
      841 tgggagaggg tcggggtgag ggcatcagcg cgcacttaac ccgcaactgc aatacgtttc
      901 atatccgtca tatagccgcg cagtttctta cctacctgct caatcgcatg gcagcgaatc
      961 gcttcgttca catcacgcag ttgcgcgtta tctaccgcac cttccggaat agctttaccc
     1021 agatcgcccg gttgcagctc tgccataaac ggtttcagca acggcacaca agcgtaagag
     1081 aacagatagt taccgtactc agcggtatca gagataacca cgttcatttc gtacagacgc
     1141 ttacgggcga tggtgttggc aatcagcggc agctcgtgca gtgattcata atatgcagac
     1201 tcttcaatga tgccggaatc gaccatggtt tcaaacgcca gctcaacgcc cgctttcacc
     1261 atcgcgatca taagtacgcc tttatcgaag tactcctgct cgccgatttt gccttcatac
     1321 tgcggcgcgg tttcgaaggc ggttttgccg gtctcttcac gccaggtcag cagtttctta
     1381 tcatcgttgg cccagtccgc catcataccg gaggagaatt cgccggagat gatgtcgtcc
     1441 atatgtttct ggaacagggg tgccatgatc tctttcagct gttcagacag cgcgtaagca
     1501 cgcagtttcg ccgggttaga gagacggtcc atcatcaggg tgatgccgcc ctgcttcagc
     1561 gcttcggtga tggtttccca accgaactga attagttttt ctgcgtatgc cggatcggtg
     1621 ccctcttcca ccagcttgtc gaagcacagc agcgagccag cctgcaacat accgcacagg
     1681 atggtttgct cacccatcag gtcagatttc acttccgcaa cgaaggacga ttccagcacg
     1741 cccgcacggt gaccaccggt tgctgccgcc catgctttag cgatcgccat gccttcgcct
     1801 ttcggatcgt tttccgggtg aacggcaatc agcgtcggta cgccgaatcc acgtttgtac
     1861 tcttcacgca cttcggtgcc agggcatttc ggcgcaacca tgactacggt gatgtcttta
     1921 cggatctgct cgcccacttc gacgatgttg aaaccgtgcg agtaacccag cgccgcgccg
     1981 tctttcatca gtggctgtac ggtgcgcact acgtcggagt gctgcttgtc tggcgtcagg
     2041 ttaaccacca aatccgcctg cgggatcagt tcttcgtaag tgcccacttt aaaaccgttt
     2101 tcggtcgctt tacgccagga tgcgcgcttc tcggcaatcg cttctttacg cagagcgtag
     2161 gagatatcga gaccagaatc acgcatgttc aggccctggt tcagcccctg tgcgccacag
     2221 ccgacgataa ccactttttt accctgaagg tagctcgcgc cgtcggcgaa ttcgtcgcgc
     2281 cccataaagc gacatttgcc cagctgtgcc agctgctggc gcaggttcag tgtattgaag
     2341 tagttagcca tggtgattcc tcgtgatgtt gtgcttctta ttgttcggtt cgcgtttgcg
     2401 agattgaatt cactatatga caggaaattt attgcggaaa ttgatatatt cacaacgtca
     2461 cattgcaatt tctgcaacgt caacattgag ggctgctcca ttggatttac gcgatctgaa
     2521 aaccttcctg catctggcgg aaagccgcca ttttggccgc agcgcgcggg cgatgcacgt
     2581 tagcccatcc acgctctcac ggcagattca gcgcctggaa gaagatcttg gtcagccgct
     2641 atttgtgcgc gataaccgca cggtgacgct gactgaagcg ggcgaagagc tgcgcgtttt
     2701 cgcccagcaa acgctgttgc agtatcagca gttgcgccac accatcgatc agcaagggcc
     2761 gtcgctctct ggcgaattac atatcttctg ctcggtgacc gctgcctaca gccatctgcc
     2821 accgattctc gaccgcttcc gcgcggaaca cccgtcggtg gagatcaaac taaccactgg
     2881 tgatgcggca gatgcgatgg aaaaggtagt cactggcgaa gcggatctgg cgattgccgg
     2941 gaaaccggag actctgcccg gcgcagtggc gttttcgatg ctggagaatc tggcagtagt
     3001 gctgattgcg cccgcgctgc cctgcccggt gcgtaaccag gtttcgtcag agaagccgga
     3061 ctggtctaca gtgccgttta tcatggccga tcagggaccg gtacgccgcc gcattgaact
     3121 gtggtttcga cgcaataaaa tcagcaaccc gatgatttac gccacggttg gcgggcatga
     3181 agcgatggta tcgatggtgg cgctcggctg tggcgtggcg ctgttgccgg aagtagtgct
     3241 ggaaaacagc cccgaaccgg tgcgtaaccg cgtgatgatt ttagagcgca gcgatgaaaa
     3301 aacgccgttt gaactgggcg tttgtgcgca gaaaaagcgg ctgcatgagc cgctgattga
     3361 ggcgttctgg aagattttgc cgaaccatta acccgccaaa aagaacctga acgccggatt
     3421 attggtttcg tcgtggcaat cgtagcccag ctcatttagc cgggtttcga aatccggttc
     3481 atggtcgccg agttcgaacg ccgccagtac gcgcccgtag tcggtgccat ggctgcgata
     3541 gtggaacaaa gaaatgttcc agtgcgtacc cagcgtgttg aggaagcgca gcagcgcgcc
     3601 cggtgattcc gggaattcga agctgtagag gcgttcctgc aacggatgtg acggacgccc
     3661 gccgaccata tagcgcacat gcaacttcgc catttcgtcg tcggagagat ccaccacgct
     3721 gtagccgccg tcgttgagca tctgcaaaat ttctttgcgc tcttcgaggc cgcggctcag
     3781 gcgcacgccg acaaagatgc aggcgttttt ggcatcggca aaacggtagt tgaactcggt
     3841 gaccgctcgc ccgccaagca gttggcagaa tttgaggaag ctgccttttt cttccggaat
     3901 ggtcaccgcc aacaacgctt cacgctgttc gcccagttcg cagcgttccg agacgtagcg
     3961 caggccgtgg aagttcacgt tggcaccgga aagaatatgc gccagccgtt cgccgcgaat
     4021 gttgtgctgg gcgatatatt ttttcattcc cgccagcgcc agcgcgccag agggttccgc
     4081 caccgcgcgc acatcttcga ataaatcctt catcgccgca cagatcgcat cactatcgac
     4141 ggtgatgatg tcgtcgagat actcctggca taaacggaag gtttcgtcac cgatgcgttt
     4201 taccgctacg ccttcagcaa atagccctac gcgcggcaga tcaaccggat gacccgcatc
     4261 cagcgctgct ttcaggcagg cggagtcttc cgcttctacg gcgatcactt tgatttgtgg
     4321 catcagttgt ttgatcagca ccgcaacgcc cgccgccaga ccgccgccgc cgactggcac
     4381 aaatacgcgg tcgagatggg cgtcctgctg gagcagttcc agcgccagcg tgccttgccc
     4441 ggcaatcacc atcggatggt cgaacggcgg cacccaggtg aacccctgct gctgtgacag
     4501 ttcgatcgct ttagctttcg cttcgtcaaa gttcgcgcca tgaagcagca cttcgccgcc
     4561 gaatccacgt accgcatcga ctttgatgtc ggcggtggcg gttggcataa cgatcagggc
     4621 cttcacgcct aaccgcgcag aagaaaacgc gacgccctgc gcgtggttac ccgcagaagc
     4681 agtgatcacg ccgtgcgctt tttgttcttc cgtcaggccc gccatcatgg cgtatgcgcc
     4741 gcgcagctta aagctgtgca ctggctggcg atcttcgcgc ttcaccagaa tgacgttatc
     4801 aagacgcgac gacagttttt ccattttttg tagcggcgta acctgcgctg cctcgtaaac
     4861 cggcgcgcgc agcactgctc ttaaatattc ggcaccttcc ggagcaccag acaggggttg
     4921 cgagtcagcc attattaacc ccccagtttc gatttatcgc gcaccgcacc tttgtcggcg
     4981 ctggttgcca ggctggcgta ggcgcgcagg gcaaaggaaa cctgacgttc acggtttttc
     5041 ggcgtccagg ctttgtcacc ccgagcttcc tgcgcttcac gacgcgccgc cagttcggca
     5101 tcgcttacct gtaactgaat gccacggttc gggatgtcga tagcgatcag gtcaccatct
     5161 tcaatcagac caatgctgcc gccgcttgcc gcttccggtg agacgtggcc gatggaaaga
     5221 ccagaggtgc cgccagagaa acgaccgtcg gtgatcagcg cacaggcttt accgagaccc
     5281 attgatttca ggaagctggt tgggtagagc atttcctgca tgccagggcc gcctttcggg
     5341 ccttcatagc ggatcaccac cacatcgccc gcgacaactt tgccaccgag gatcgcttct
     5401 accgcgtcgt cctggctttc gtacactttt gccgggccgg tgaatttgag gatgctgtca
     5461 tcgacgcccg cggttttcac gatgcagccg ttttcagcaa aattaccgta gagcaccgcc
     5521 aggccgccgt ctttgctgta ggcgtgttcc agcgagcgga tacagccgtt tgcgcgatcg
     5581 tcgtccagcg tatcccaacg gcaatcttgc gagaatgcct gggtggtacg gatacccgca
     5641 gggccggcgc ggaacatatt ttttaccgcg tcatcctggg tcagcataac gtcgtattgc
     5701 tccagcgttt gcggcaacgt caggccgagc acgtttttca catcacggtt cagtaacccc
     5761 gcgcgatcca gttcgccgag aataccgata acaccaccag cacggtgaac atcttccata
     5821 tggtatttct gggtgctcgg cgcaacttta cacagctgtg gaaccttgcg ggaaagctta
     5881 tcgatatcac tcatggtgaa gtcgatttcc gcttcctgcg ccgccgccag caggtgaagt
     5941 acggtgttag tcgatccacc catcgcgata tccagcgtca tggcgttttc aaacgccgcc
     6001 ttactggcga tattacgcgg cagtgcactt tcgtcgtttt gctcgtaata acgtttggtc
     6061 aattcaacaa tgcgtttacc agcattaagg aacagctgct tacggtccgc gtgggttgcc
     6121 agcagcgagc cgttgcctgg ctgcgacaaa cccagcgctt cggtcaggca gttcattgag
     6181 ttagcggtaa acatcccgga gcaggaaccg caggttgggc aggcggaacg ttcaacctga
     6241 tcgctctggg agtcagaaac tttcgggtct gcgccctgga tcatcgcatc aaccagatcg
     6301 agtttgatta tctgatcgga cagtttggtt ttcccggcct ccatcgggcc gccggaaaca
     6361 aagatcaccg gaatattcag gcgcagggaa gccatcagca tccccggggt gattttgtcg
     6421 cagttagaga tgcagaccat ggcgtcggcg cagtgggcgt tgaccatata ctcaacggaa
     6481 tcagcgatca gttcgcgaga tggaagtgaa taaagcatcc ccccgtggcc catggcaatc
     6541 ccatcatcca ccgcaatggt gttgaactct ttggcaacgc cgccagccgc ttcaatttgt
     6601 tcggcgacca gtttaccgag atcgcgcaga tggacgtgac ccggtacaaa ttgggtgaac
     6661 gagttcacaa ccgcgataat cggcttgcca aaatcggcgt cggtcattcc ggtggcgcgc
     6721 cacagcgcac gagcacccgc catattacga ccatgagtgg tggtggcgga acggtactta
     6781 ggcatacttt atttactccc agtgtctgtc tcgtaaatgg gacggtgcgt gccgtcccat
     6841 tttttgtatt tattgattaa cttgatctaa ccagccccat ttatcttcgg tttcgccagt
     6901 gaagaggccg aagaaggctt gctgaatgcg tttggtaacc gggccacaac ggccttcgcc
     6961 aacctgaata ccgtctacgc tgcgcactgg cgtgatttct gccgcagtac cggacataaa
     7021 cacttcatcc gccaggtaca gggattcgcg cgacagcacc tgctcacgca cttcaattcc
     7081 cagctctttc gccagtttga tgatggcatc acgggtaata cccggcagcg cggatgaggt
     7141 aaacggtggg gtgaacagca cgccgtcttt cacttcaaac aggttttcac ctgcgccttc
     7201 agagatgtaa ccgttcacat ccagcgcgat accttcctga taaccgtggc ggcgcgcttc
     7261 gctacccacc agcagggaag agaggtagtt accaccggct tttgccgccg tcgggatggt
     7321 gtttggtgct gcgcggttcc aggaggaaac catcgcatcg atcccctgct ccagcgcttc
     7381 tgcgcccaga tacgctcccc acgggaaagc agcgataatc acgtcggttg agtatcccgc
     7441 tggcgggttt actcccatgc caacatcacc gacgaagatc agcggacgga tataggcgct
     7501 ggtgagatta tttttgcgga tcacgtcacg acaagcttcc atcagctcat caatgctctg
     7561 cgaaaccggg aagcgataga ttttggcgga gtcatgcaga cgctgcatat gctcacgatg
     7621 gcggaataca accggtcctt tgtgcgagtc gtagcaacgg atgccttcaa aaaccgaagt
     7681 gccatagtgc agcgcgtgcg acatcacatg caccttcgcg tcttcccagc gaaccatctc
     7741 cccattgaac caaatgtaat cagctttctt cgtggtcatt tttatattcc ttttgcgctc
     7801 aggcgcggat ttgttgtgat gtggttgtgc tctggcagat ggcaacgtgt gcgacgtcca
     7861 ccagtttatt taactgacta aacagtaagt cgaccgaccg tgggctggca acggtcaatt
     7921 cgatatttat attttgtgca tcgctggcgg cggccatatt cattgagcag acgtggaaac
     7981 cacgatgacg caccacgcgt aaaacacgtt ctaaggtttc cggattgaag cgagccgata
     8041 cattgacctg atattgcatc atgataattt ctccaacatt tctgaattac tggcgccagg
     8101 cggcaccagc ggccagacgt tctcaagttc gtcgattgag acatgaagca ggtatggccc
     8161 atcactgttc agcatggtgt cgagtgccgc ttcaacctgg tctttacggg tgatgtgttg
     8221 gccagggatg ccgaaggcgc tggctaacat gaggaaatcg gggttatcag taagggtggt
     8281 ttcgctgtat cgttcctgaa aaaacagttg ctgccattgt cgaaccatcc ctaaccgttg
     8341 gttatcgagt aagacgattt tcaacggtaa ctgcttgcgt tttacggtgc ccagctcttg
     8401 cacattcatc atgaaagagc cgtcaccgga gatacagacg acggtatcgt tcggtcgcgc
     8461 gacttgtgcg ccaaccgccg ctggtaaacc aaaacccata gtgcctaagc cgctggaggt
     8521 gatgaaattt tccgggcgag tgtgggcgat gtgttgcgca gcccacatct ggtgctgccc
     8581 cacatctgtg gtcacgacgc aatccgcagg tttacgatcc gacagttgtt ttaacaacaa
     8641 cggcgcgtag atagcgtcgc cgggatggtc gtaacgccag gcatgttcat cacgcagctg
     8701 cgcgcagtat tgctgccagt cattgatatt taacggctgc tgtaattctg gtaacagagc
     8761 atttaaatca ccttgtaatg ccacatgtgc ctgacgcagc ttgttcattt ctgccgggtc
     8821 gatatccata tggataacgc tggcgtgtgg tgcgaaggtg ttcagtttgc cggtcacccg
     8881 gtcatcaaaa cgtgcaccca cggcgatcag caagtcgcac tcctgcaccg cgaagttcgc
     8941 cgctttggtg ccatgcattc ccagcatgcc cagatagtac ggataatctg cttcaactgc
     9001 gcccagccct ttcagcgtgc aggtggcagg catttttgtg gtagcgagaa attctcgtaa
     9061 agcaggaact gcctgcgcca tacccacgcc accaccaacg tacagcatcg gtttttgcgc
     9121 ttttgccagc atctggcgcg cttgctcaac ttcggcatgt gggaaagtca cttcgttttc
     9181 aacggtggtg aaccacggtt ccaggtcacc gctggctagc tggatatctt ttgggatatc
     9241 gaccagaacc ggaccaggac gacctgagct ggcaacgtcg aatgcttcag ccataatgcg
     9301 cggcaactct tccagcgact gcaccagaaa gctgtgcttg gtacaggcta acgacaatcc
     9361 caggacatcc acttcctgaa atgcatcagt gccgataaac ggtgcggaca cttgaccggt
     9421 gatggcaaca acagggatag aatctaacag tgcgtccgca agcccggtta tcaggttggt
     9481 tgcgcccgga ccagacgtgg cgatacatac gccagttttg ccggtagcac gggcataacc
     9541 gatagccgcc attgccgcac cctgctcatg tcggcacagc aagtgctcca cgccgccgtc
     9601 atacaatgca tcgtaaaccg gcataattgc gccacccgga taaccgaaaa cggtgttcac
     9661 accctgtgcc cgcaacgcat gtaccaccca ctgtgcgcca ttcatagtta gttccccgtg
     9721 ctgaatgttg agaaacagaa ttttgtgctg ttattcattg tctgctcctc ggttatgttt
     9781 ttaaggtcaa aaaaaacccc cggacctttc ggtgcggggg tcttagttcg ttaaggcttg
     9841 atttctaagc ctttcctcgt ccaagtgcag ccccgcacgg tgggataata atcaccacca
     9901 cgctaatcac gaccaggcta atcactcgta gaagggctgt cattttgtct tttcttgcat
     9961 cttgttcgaa ggaatgccta aagagttacc ataggttttg taaatagtac aagatatttt
    10021 ttggccgatt tttatgatgc gtttaataat ttcaatataa gttgttgttt tttaaaaata
    10081 aaatagtgag taaaaaaatt caatttcagt atgaatgttg ttcccttcct cgtagttcat
    10141 cctttgtccg ctgcattttt gcgagcatca ttccaccgcc agaacaactc aacttcactt
    10201 gcacaaataa acaggaatca gccactaaaa ttcacgaatt agcgggctac agcagcgata
    10261 tcttatgcac ataatggtga ctcaaggagg gcttatgtca ctgtcaattg ttcatacccg
    10321 cgcagccctg ggagtaaatg cgcccccgat cactgttgag gtacatatca gtaaaggtct
    10381 acccggctta acgaaggtgg gcttaccaga aacaacggta aaagaagctc gcgatcgcgt
    10441 gcgcagcgcc attatcaata gcggatatga atatccggcg aaaaaaatca ccatcaacct
    10501 ggctccagct gatctgccaa aagaaggggg acgatatgat ttacctatcg ccattgcgtt
    10561 gctggcggcc tcagaacagc ttacagccaa taagttagat gaatatggat tagtcggaga
    10621 actggcgctt acaggcgctc tgcgtggcgt tcccggcgca atctccagta caactgaagc
    10681 tattaagtcg ggcagaaaaa ttatcgtcgc gaaagataac gaagatgaag tggggctaat
    10741 taacggtgaa ggatgcctga tagccgatca tctgcaagct gtctgtgcgt ttctggaagg
    10801 taagcacgct ctcgaacgcc cgaaaccaac tgatacagta tcccgggcgc tacaacatga
    10861 tctcagtgat gttatcggtc aggaacaagg aaagcgagga ctggaaatta ccgccgctgg
    10921 cgggcacaac cttttactga ttgggccgcc gggaacaggt aaaacaatgc tcgccagccg
    10981 tattaatggc ctgttgccag atttaagcaa tgaagaggca ctggagagtg ctgcgatatt
    11041 aagtctggta aacgctgaat cagtacaaaa acaatggcgg cagcgcccgt tccgctcacc
    11101 tcatcacagt gcatcgttaa ctgcgatggt aggcggtggc gcaattccag ggcccgatga
    11161 aatttcgctg gcgcataacg gcgtgctttt tcttgatgag ttacctgaat ttgaaaggcg
    11221 tacactggat gccttgcgag agccgattga atccgggcag atccatcttt cacgcacgcg
    11281 agcaaaaata acctatccag cccgtttcca gcttgtcgcg gcgatgaatc ccagccctac
    11341 cggacattat cagggaaacc ataaccgctg cacaccagag caaacgctgc gttatctcaa
    11401 ccgactctct ggtccctttc tcgaccgctt cgatctctca ctggagatcc cattaccgcc
    11461 tcccggtatt ttgagtaaaa cggtagtgcc gggagaaagc agcgccaccg ttaaacaacg
    11521 cgtaatggcc gccagagagc gccaatttaa gcggcagaat aaactgaacg cctggctgga
    11581 tagtccggaa atacgccaat tctgcaaact tgagagcgaa gatgcgatgt ggctggaaga
    11641 aacgctgatc catctggggt tatcgattcg tgcctagcag cggttattga aagttgcacg
    11701 aaccattgct gatattgatc agtctgacat tatcacacgt caacatttgc aggaggcagt
    11761 tagctatcga gcgattgacc gtttgctcat ccatctgcag aaactactga cataaaaaaa
    11821 gggcatttcg ccctttttat taatcgtcag aatcggtgta gtcttcagca ccttcaacct
    11881 gcggtttacc gccggaaagg gtgtgaaaac gttttggacg cttaatacgc gtcatatact
    11941 tggaccacac gcgttctgct tctgtcactg gctcacgttc gccacggcat actgctacga
    12001 agagtttctc ttcctcggta accggctcgc gtttgccaag atccaactca ttgaaggcat
    12061 aaccatgacg ctcaagcagt tgtgcctctt tgatggtgaa atcaccatga cgagagaatc
    12121 cacgtggata atgtttattg tcgaaatatc gattagtcgt cgtaaagctt tccgccatcc
    12181 tgcacgctcc taattctttg accgagctag ttatggcgcg gagtattagt tacgcttgac
    12241 agagtgtaaa acaaaacatt taaatcataa cgacaaataa ttttgcggag agcactgtgg
    12301 atacggaatt gttaaaaact ttcctggaag ttagccgaac gcgtcacttt ggtcgagcgg
    12361 ctgaatcgct ctatctgacc cagtcagcag tgagctttcg aatcagacaa ctggaaaatc
    12421 aactgggtgt gaaccttttc acccgccaca gaaacaatat ccgtttaacc gctgccggtg
    12481 aaaaactact gccttatgca gaaacgctca tgagcacctg gcaggccgcc cgcaaggagg
    12541 tggcgcatac ctcacgacat aacgagtttt ctatcggtgc cagcgcctca ttgtgggaat
    12601 gtatgcttaa tcagtggctg ggacgtttgt atcaaaatca ggatgcccat acaggcttac
    12661 agttcgaagc gcgaattgcc caacggcagt ctctggtaaa acagctgcat gaacgccagc
    12721 ttgatcttct tattaccact gaagcgccca aaatggacga atttagtagt cagttgctgg
    12781 gatatttcac tttagcgctt tataccagtg ccccttcaaa actaaaggga gatcttaatt
    12841 atctgcgact tgagtggggg ccagattttc aacagcatga ggcaggtttg atcggtgctg
    12901 acgaagtgcc cattctgaca accagttctg ctgaactggc acagcaacag attgcgatgc
    12961 ttaatggttg cacctggcta cccgtcagct gggcgcgtaa aaaaggcggc ctgcataccg
    13021 ttgtcgatag cacaacactt tcacggccgc tttatgccat atggctgcaa aatagcgata
    13081 aaaatgcgtt gattcgcgat cttttgaaaa ttaacgtgct ggatgaagtg tattaatatg
    13141 aatggctggc aaggatgccg gtagaaggat ttacttcgga gagggttatt tcagataaaa
    13201 aaaatcctta gctttcgcta aggatgattt ctggcagggg cggagagact cgaactccca
    13261 acacccggtt ttggagaccg gtgctctacc aattgaacta cgcccctaat tag
//
LOCUS       DAJTMC010000113        13100 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.113, whole genome shotgun sequence.
ACCESSION   DAJTMC010000113 DAJTMC010000000
VERSION     DAJTMC010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13100
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.113"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            179..1153
                     /gene="cysB"
                     /locus_tag="OO130_002896"
     CDS             179..1153
                     /gene="cysB"
                     /locus_tag="OO130_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HCR5366224.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QTRYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            1448..1612
                     /locus_tag="OO130_002897"
     CDS             1448..1612
                     /locus_tag="OO130_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HCR5366225.1"
                     /translation="MLISDGDYMRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVV
                     VALLIWKVWG"
     gene            1615..1782
                     /locus_tag="OO130_002898"
     CDS             1615..1782
                     /locus_tag="OO130_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5366226.1"
                     /translation="MVGQEQLESSPLCQHSDNETETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            1896..1991
                     /locus_tag="OO130_002899"
     CDS             1896..1991
                     /locus_tag="OO130_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5366227.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            2155..4830
                     /gene="acnA"
                     /locus_tag="OO130_002900"
                     /pseudo
     CDS             2155..4830
                     /gene="acnA"
                     /locus_tag="OO130_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
     gene            complement(4894..5484)
                     /gene="ribA"
                     /locus_tag="OO130_002901"
     CDS             complement(4894..5484)
                     /gene="ribA"
                     /locus_tag="OO130_002901"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HCR5366228.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            5654..6418
                     /gene="pgpB"
                     /locus_tag="OO130_002902"
     CDS             5654..6418
                     /gene="pgpB"
                     /locus_tag="OO130_002902"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HCR5366229.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLLWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            6567..6875
                     /gene="lapA"
                     /locus_tag="OO130_002903"
     CDS             6567..6875
                     /gene="lapA"
                     /locus_tag="OO130_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HCR5366230.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            6882..8051
                     /gene="lapB"
                     /locus_tag="OO130_002904"
     CDS             6882..8051
                     /gene="lapB"
                     /locus_tag="OO130_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HCR5366231.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNRARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            8244..8981
                     /gene="pyrF"
                     /locus_tag="OO130_002905"
     CDS             8244..8981
                     /gene="pyrF"
                     /locus_tag="OO130_002905"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HCR5366232.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSDAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            8981..9307
                     /gene="yciH"
                     /locus_tag="OO130_002906"
     CDS             8981..9307
                     /gene="yciH"
                     /locus_tag="OO130_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HCR5366233.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGIDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(9433..9651)
                     /gene="osmB"
                     /locus_tag="OO130_002907"
     CDS             complement(9433..9651)
                     /gene="osmB"
                     /locus_tag="OO130_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HCR5366234.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(9920..10669)
                     /locus_tag="OO130_002908"
     CDS             complement(9920..10669)
                     /locus_tag="OO130_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR5366235.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEVIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            complement(10759..10932)
                     /locus_tag="OO130_002909"
     CDS             complement(10759..10932)
                     /locus_tag="OO130_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5366236.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            complement(11079..13064)
                     /gene="pdeR"
                     /locus_tag="OO130_002910"
     CDS             complement(11079..13064)
                     /gene="pdeR"
                     /locus_tag="OO130_002910"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HCR5366237.1"
                     /translation="MKTVRESTTLYNFLGSHNPYWRLTESSDVLRFSTTETTEPDRTL
                     QLSAEQAARIREMTVITSSLMMSLTVDESDLSVHLVGRKINKREWAGNASAWHDTPAV
                     ARDLSHGLSFAEQVVSEAHSAIVILDSRGNIQRFNRLCEDYTGLKEHDVIGQSVFKLF
                     MSRREAAASRRNNRVFFRSGNAYEVELWIPTCKGQRLFLFRNKFVHSGSGKSEIFLIC
                     SGTDITEERRAQERLRILANTDSITGLPNRNAMQDLIDHAINHADNNKVGVVYLDLDN
                     FKKVNDAYGHLFGDQLLRDVSLAILSCLEHDQVLARPGGDEFLVLASNTSQSALEAMA
                     SRILTRLRLPFRIGLIEVYTSCSVGIALSPEHGSDSTAIIRHADTAMYTAKEGGRGQF
                     CVFTPEMNQRVFEYLWLDTNLRKALENDQLVIHYQPKITWRGEVRSLEALVRWQSPER
                     GLIPPLDFISYAEESGLIVPLGRWVILDVVRQVAKWRDKGINLRVAVNISARQLADQT
                     IFTALKQVLQELNFEYCPIDVELTESCLIENDELALSVIQQFSQLGAQVHLDDFGTGY
                     SSLSQLARFPIDAIKLDQVFVRDIHKQPVSQSLVRAIVAVAQALNLQVIAEGVESAKE
                     DAFLTKNGINERQGFLFAKPMPAVAFERWYKRYLKRA"
BASE COUNT         3334 a         3083 c         3296 g         3387 t
ORIGIN      
        1 gggtcgcgaa agcggccctt ttttattgca tattattttt tcttcacacc tatacactaa
       61 ggctataaat gatatagtgg ttatagttag cacctttttt attattaaat cgtattagtc
      121 acccgccagg tgtgacgaaa aaacgatgtt ctgatggcgt ctaagtggat ggtttaacat
      181 gaaattacaa caacttcgct atattgttga ggtggtcaat cataacctga atgtctcatc
      241 aacagcggaa ggactttaca catcacaacc cgggatcagt aaacaagtca gaatgctgga
      301 agacgagcta ggcattcaaa ttttttcccg aagcggcaag cacctgacgc aggtaacgcc
      361 agcagggcaa gaaataattc gtatcgctcg cgaagtcctg tcgaaagtcg atgccataaa
      421 atcggttgcc ggagagcaca cctggccgga taaaggctca ctgtatatcg ccaccacgca
      481 tacccagaca cgctacgcat taccaaacgt catcaaaggc tttattgagc gttatcctcg
      541 cgtttctttg catatgcacc agggctcgcc gacacaaatt gctgatgccg tctctaaagg
      601 caatgccgat ttcgctatcg ccacagaagc gctgcatctg tatgaagatt tagtgatgtt
      661 accgtgctac cactggaatc gggctattgt agtcactccg gatcacccgc tggcaggcaa
      721 aaaagccatt accattgaag aactggcgca atatccgttg gtgacatata ccttcggctt
      781 taccggacgt tcagaactgg atactgcctt taaccgcgca gggttaacgc cgcgtatcgt
      841 tttcacggca acggatgctg acgtcattaa aacttacgtc cggttagggc tgggggtagg
      901 ggtcattgcc agcatggcgg tggatccggt cgccgatccg gaccttgtgc gcgttgatgc
      961 tcacgatatc ttcagccaca gtacaaccaa aattggtttt cgccgtagta cttttttgcg
     1021 cagttatatg tatgatttca ttcagcgttt tgcaccgcat ttaacgcgtg atgtcgttga
     1081 tgcggctgtc gcattgcgct ctaatgaaga aattgaggtc atgtttaaag atataaaact
     1141 gccggaaaaa taatttcttg cgttattttc ggcacctttt atgtagcgaa ggtgccggaa
     1201 tatattctct tttgttactt atttttatac cgtgcacaat atttttatcc ctcatattta
     1261 tagggtaaat tacctgtaaa gtagtgtcta attacccgcc tcgcaaattc cagttttcaa
     1321 atatctattt caggtcaaaa gattgaatta ttcactggag acgattcgta aattcgctgg
     1381 atttttgact aaagtttctt taggatttgt ctcatcgtat gattaattac accaatcgtt
     1441 caacgtcttg ctgataagtg atggcgatta tatgaggtta gcaatgcctt ctggaaatca
     1501 ggaaccccgc cgcgatcctg aattaaaacg taaagcctgg ctggcggttt ttcttggttc
     1561 tgcacttttc tgggtggttg tcgcactgct gatttggaaa gtgtggggat aactatggtc
     1621 ggtcaggagc aactggagtc gtcaccatta tgccagcata gtgacaatga aacagaaacg
     1681 aaacgggaat gttccgtcgt tattccagac gactggcaac taacatcgca gcagcaagcc
     1741 tttatagaac tgtttgctga agatgatcag ccgaaacaat aattatcatc attcttatta
     1801 cccattttta atgaattaaa gggcttttaa tacaccgcag caataacagc ttgagttatc
     1861 tcaacacaaa ataataaccg ttaagggtgt agcctatgat caacacaaat atgaaatatt
     1921 ggtcctggat gggcgcgttt tctctgtcga tgctcttctg ggccgaactc ctctggatca
     1981 ttactcactg atccttgacc ccgctgcggc ggggttgtca tttgctttgc cacaaggttt
     2041 ctcctctttt atcaatttgg gttgttatca aatcgttacg cgatgtttgt gttatcttta
     2101 atattcaccc tgaagagaat cagggcttcg caaccctgtc attaaggagg agctatgtcg
     2161 tcaaccctac gagaagccag taaggacacg ttgcaggcca aagataaaac ttaccactac
     2221 tacagcctgc cgcttgctgc taaatcactg ggcgatatca cccgtctacc caagtcactc
     2281 aaagttttgc tcgaaaacct gctgcgctgg caggatggta actcggttac cgaagaggat
     2341 atccacgcgc tggcaggatg gctgaaaaat gcccatgctg accgtgaaat tgcctaccgc
     2401 ccggcaaggg tgctgatgta ggactttacc ggcgtacctg ccgttgttga tctggcggca
     2461 atgcgcgaag cggttaaacg cctcggcggc gatactgcaa aggttaaccc gctctcaccg
     2521 gtcgacctgg tcattgacca ctcggtgacc gtcgatcgtt ttggtgatga tgaggcattt
     2581 gaagaaaacg tacgcctgga aatggagcgc aaccacgaac gttatgtgtt cctgaaatgg
     2641 ggaaagcaag cgttcagtcg gtttagcgtc gtgccgccag gcacaggcat ttgccatcag
     2701 gttaacctcg aatatctcgg caaagcagtg tggagtgaat tgcaggacgg tgaatggatt
     2761 gcttatccgg atacactcgt tggtactgac tcgcacacca ctatgatcaa cggccttggc
     2821 gtgctggggt ggggcgtggg tgggatcgaa gcagaagccg caatgttagg ccagccggtt
     2881 tccatgctta tcccggacgt agtgggcttt aaacttaccg gtaaattacg tgaaggtatt
     2941 accgccacag acctggttct cactgttacc caaatgctgc gcaaacatgg cgtggtgggg
     3001 aaattcgtcg aattttatgg tgatggtctg gattcactac cgctggcgga tcgcgccacc
     3061 attgccaata tgtcgccaga atatggtgcc acctgtggct tcttcccaat cgatgctgta
     3121 accctcgatt acatgcgttt aagcgggcgc agcgaagatc aggttgagtt ggtcgaaaaa
     3181 tatgccaaag cgcagggcat gtggcgtaac ccgggcgatg aaccgatttt taccagtacg
     3241 ttagaactgg atatgaatga cgttgaagcg agcctggcag ggccaaaacg tccacaggat
     3301 cgcgttgcac tgcccgatgt accaaaagca tttgccgcca gtaacgaact ggaagtgaat
     3361 gccacgcata aagatcgcca gccggtcgat tatgttatga acggacatca gtatcagtta
     3421 cctgatggcg ctgtggtcat tgctgcgata acctcgtgca ccaacacctc taacccaagt
     3481 gtgctgatgg ccgcaggctt gctggcgaaa aaagccgtaa ctctgggcct caagcggcaa
     3541 ccgtgggtca aagcgtcgct ggcaccgggt tcgaaagtcg tttctgatta tctggcaaaa
     3601 gcgaaactga caccgtatct cgacgaactg gggtttaacc ttgtgggata cggttgtacc
     3661 acctgtattg gtaactctgg gccgctgccc gatcctatcg aaacggcaat caaaaaaggc
     3721 gatttaaccg tcggtgcggt gctgtccggc aaccgtaact ttgaaggccg tatccatccg
     3781 ctggttaaaa ctaactggct ggcctcgccg ccgctggtgg ttgcctatgc gctggcggga
     3841 aatatgaata tcaacctggc tgctgagcct attggtcatg atcgtaaagg cgacccggtt
     3901 tatctgaaag atatctggcc atcggcacag gaaattgccc gtgcggtaga acaagtctcc
     3961 acagaaatgt tccgtaaaga gtacgcagaa gtttttgaag gcacagcaga gtggaaggaa
     4021 attaacgtca cacgatccga tacctacggt tggcaggagg attcaaccta tattcgctta
     4081 tcgcctttct ttgatgaaat gcaggcaaca ccagcacctg tggaagatat tcacggtgcg
     4141 cggatcctcg caatgctggg ggattcagtc accactgacc atatctctcc ggcgggcagt
     4201 attaagcccg acagcccggc gggtcgatat ctacaaggtc ggggtgttga gcgaaaagac
     4261 tttaactcct acggttcgcg gcgtggtaac catgaagtga tgatgcgcgg caccttcgcc
     4321 aatattcgca tccgtaatga aatggtgcct ggcgttgaag gggggatgac gcggcattta
     4381 cctgacagcg atgttgtctc tatctatgat gccgcgatgc gctataagca ggagcaaacg
     4441 ccgctggcgg tgattgccgg gaaagagtat ggatcaggct ccagccgtga ctggacggca
     4501 aaaggtccgc gtctgcttgg tattcgtgtg gtgattgccg aatcgtttga acgaattcac
     4561 cgttcgaatt taattggcat gggcatcttg ccgctggaat ttccgcaagg cgtgacgcgt
     4621 aaaacgttag ggctaaccgg ggaagagaag attgatattg gcgacctgca aaatctacaa
     4681 cccggcgcga cggttccggt gacgcttacg cgcgcggatg gtagccagga agtcgtaccc
     4741 tgccgttgtc gtatcgacac cgcgacggag ttgacctact accagaacga cggcattttg
     4801 cattatgtca ttcgtaatat gctgaagtaa caagtatttg cttgccggtt attttgcttc
     4861 cggcaagcaa attaattcca caatgcgaga gggttatttg ttcagcaaat gacccatttt
     4921 ctcggctttg gtatcgagat aatgttcgtt attggggtta cgacctacaa tcaatggtac
     4981 gcgttcaaca atattaatcc ctgcttcggt cagaatttcg acttttttcg ggttattggt
     5041 taacaagcgg acttcattga cgccaaggag tttgaacata tcagcgcaaa gagtgaagtc
     5101 gcgctcatca gcggcgaagc ctaactggtg gttagcctct acggtatcgt aaccttgatc
     5161 ctgcagtgcg taagcgcgga ttttattcag cagaccaatg ttacgacctt cctgacggtg
     5221 atacagcaaa ataccacggc cttcctcggc aatttgcgtc aatgccgctt cgagctggaa
     5281 gccacaatcg cagcgcaagc tgaacagggc gtcaccggtc agacattcgg aatggacgcg
     5341 cgcaagtacc ggggtatgcc cggaaatatc gccatagact agcgcgacat gatcgtgtcc
     5401 ggttgccagt tcttcaaatc ccaccatcag gaaatcgccc catggggttg gcagtttggc
     5461 ttctgccaca cgtttaagct gcatgaaatt ctccagataa tgctggttct gtattggctt
     5521 attttgccat aacgagaagg ggttcaccta atcacgacgc gtcgatcgtt cacggaatgg
     5581 cacaaatctg tcaatttttc ctggaactgg cgttttcagt tatgattgtg ggacttatca
     5641 aaaaggagag gccatgcgtt cgattgccag acgtaccgca gtgggagctg cactattgct
     5701 tgtcatgcca gtagccgtat ggatttctgg ctggcgttgg caacctggag aacaaagttg
     5761 gctactaaaa gcggcttttt gggttactga aactgtcacc cagccctggg gcgtcattac
     5821 acatttgatt ttattcggct ggtttctctg gtgtctgcgt tttcgcatta aggctgcctt
     5881 tgtattattt gccattctgg cggccgcaat ccttgtggga caaggcgtta aatcctggat
     5941 caaagacaaa gtccaggaac cacgaccttt tgttatctgg ctggaaaaaa cacatcatat
     6001 tccggttgat gagttctaca ctttaaagcg agcagaacgc ggaaatctag tgaaagaaca
     6061 gttggctgaa gagaaaaata tcccacaata tttgcgttca cactggcaga aagagacggg
     6121 gtttgccttt ccttccggtc acacgatgtt tgctgccagt tgggcactgc tggccgttgg
     6181 tttgctgtgg ccgcgtcggc gaacgttaac cattgctatc ttgctgctct gggcaacggg
     6241 agtcatggga agccgcctgc tgctcgggat gcattggcca cgcgatctgg tagtagctac
     6301 attgatttcg tgggcgctgg tggcggtggc aacgtggctt gcgcaacgaa tttgtgggcc
     6361 attaacacca cctgcggaag aaaatcgcga aatagcgcaa cgagaacaag aaagttaacg
     6421 ctggttgatt ttccgaattt agcccttaaa tcatcaacaa tgcgcgtgga tgccatttcg
     6481 cagacggcgc gaaaatggta ctttaaaggg ctattgcggt aagttgacca taatttattc
     6541 gctctaacca cataacggga agtaatgtga aatatttact cattttctta ctggtgttag
     6601 cgatcttcgt gatttcggtc acgttgggtg cgcagaacga tcaacaggtg acgtttaatt
     6661 atctgttagc gcaaggggag taccgtattt ccacattgct ggcggtattg tttgctgcgg
     6721 ggtttgctat cggttggttg atttgtggcc tgttctggct gcgagttcgt gtttccctgg
     6781 cgcgcgctga acgtaaaata aagcgactgg aaaaccagct ttcacccgcg actgacgtgg
     6841 ctgtagtgcc gcactcgtca gcggcgaagg aataactttc tatgctggag ttgttgtttc
     6901 tgcttttgcc tgtagccgct gcctatggct ggtatatggg ccgcagaagt gcgcaacaaa
     6961 acaagcaaga tgaagccaac cgcttgtcgc gtgattacgt agcgggggtt aacttcctgc
     7021 ttagtaatca acaggataaa gcggtagacc tgtttctcga tatgcttaaa gaggatacag
     7081 gcaccgttga agcccacctt acgctcggaa acctgttccg ttcgcgtggc gaagttgatc
     7141 gcgctattcg catccatcag accctaatgg aaagcgcctc gctgacctat gaacagcgtc
     7201 tgttggcgat tcaacaacta gggcgtgatt acatggccgc cgggttatac gaccgcgcgg
     7261 aagacatgtt caatcagctg accgatgaaa ctgacttccg cattggcgct ctgcaacagt
     7321 tgctacaaat ctaccaggct accagtgagt ggcagaaagc aattgatgtt gccgaacgcc
     7381 tggtgaagct gggtaaagat aaacagcgcg tcgaaattgc ccatttctac tgtgagttag
     7441 ccctgcagca tatggccagc gacgatctcg atcgtgccat gaccttgcta aaaaaagggg
     7501 cggcggcaga taaaaacaga gcccgcgtat ccatcatgat gggacgcgtg tttatggcga
     7561 aaggagaata cgccaaagcc gtcgaaagtt tgcaacgcgt gatatcccag gacagagaac
     7621 tggtcagcga aacgctggaa atgttgcaaa cctgctatca gcagttgggt aaaactgccg
     7681 aatgggcaga attcctgcaa cgcgcggtgg aagagaacac cggtgccgat gctgaattga
     7741 tgcttgctga tatcatcgaa gcgcgcgacg gtagtgaggc cgcacaggtc tatattacgc
     7801 gccagcttca gcgtcatccg accatgcgtg tgttccataa gttaatggat taccacttaa
     7861 atgaagcgga agaagggcgt gccaaagaga gcctgatggt gctgcgtgac atggttggcg
     7921 aaaaggtacg tagtaagcct cgttatcgct gccagaaatg tggttttacc gcatacaccc
     7981 tctactggca ttgtccgtct tgtcgggcct ggtcaaccat taaaccgatt cgcggtcttg
     8041 atggcctgta atttttaaaa aaatccgact ttagttacaa catgctagtt attgatgttc
     8101 cattgtgctc cggcaacgac ggagcagaaa aagcctgcca ggggaaaaat cgcaactgtt
     8161 aattttttat ttccacgggt agaatgctcg ccgtttacct gtttcgcgcc acttccggtg
     8221 cccatcatca agaaggtctg gtcatgacgt taactgcttc atcttcttcc cgcgctgtta
     8281 cgaattctcc tgtggttgtt gcccttgatt atcataatcg tgatgacgcg ctgtcctttg
     8341 tcgacaagat cgacccacgc gattgtcgtc tgaaggtcgg caaagagatg tttacattgt
     8401 ttgggccaca gtttgtgcgc gaacttcaac agcgtggttt tgatatcttt cttgacctga
     8461 aattccacga tattcctaac actgcagcgc acgctgtcgc tgctgcagct gacttaggcg
     8521 tgtggatggt gaatgttcat gcctctggtg gggcgcgtat gatgaccgca gcgcgtgagg
     8581 cactggttcc gtttggcaaa gatgcaccgc ttttgattgc tgtaacagtg ttgaccagca
     8641 tggaagctag cgacctggcc gatcttggcg tgacactgtc acctgcagat tatgcagaac
     8701 gtctggcggc actgacgcaa aaatgtggcc ttgatggtgt ggtgtgttct gctcaggaag
     8761 ctgtgcgctt taaacaggta ttcggtcagg agttcaaact ggttacaccg ggcattcgtc
     8821 cgcaggggag tgatgctggt gaccagcgcc gcattatgac gccagaacag gcgttggcgg
     8881 ctggtgttga ttatatggtg attggtcgcc cggtaacgca atcggtagat ccagcgcaaa
     8941 cactgaaagc gatcaacgcc tctttacagc ggagtgcatg atgagtgatt ccaacagccg
     9001 tctggtctac tcaacggaaa ccggacgtat tgatgagcca aaagcggccc cagtacgccc
     9061 taaaggcgat ggtgtggtgc gtattcagcg tcagaccagt ggacgtaaag gtaagggcgt
     9121 ttgcctgatt accggtatcg atctcgatga tgccgaactg acaaaacttg cagcggaact
     9181 gaagaaaaaa tgcggctgcg gcggagcagt taaagatgga gttattgaaa tccagggcga
     9241 taagcgtgat ttattaaaat cactacttga agcgaaaggg atgaaggtaa aactcgcagg
     9301 cggttaacat aaaaagccac ggatatatcc gtggctttcg aatattttac tgtgtgtatt
     9361 attcagttat ttcgtactga acaaaatcaa gcgtattata attgcgagtc gataatcgac
     9421 atttatttac gattatttac cgacctggtg accaataaca ccaccgacag ctgcaccacc
     9481 taatgtaccc aacgtactgc catcggtcag tactgcaccg cctaatgccc ctgcacccgc
     9541 gccgattgcg gtgttgcggt cccgtttaga ccagttagaa caggcactca gagacattgc
     9601 caaagtaatt gccagaacag ccgcggtcat ttttttgctc gttacaaaca taatactctc
     9661 tcctgaattt atgattcacg gaagtaagct ctctataact ataatagcta agaataagcc
     9721 tggtgaaatt attccgtgaa atctgctcgc gcgcagggtt atatcacgca ggtgataatg
     9781 acttcctgtt atatcgctga taataatttt atatcttaag cgtgttaata acaggtaaat
     9841 agtcttaatt atcaaccagg aatcatctta gagcggatga tttgccaaac tgcaaatcat
     9901 ccgtagagaa gggaaatggt taaatgtcaa cgacgtgaac ggtgagttta ctgtgctcaa
     9961 gatgcatcag atcgctggcg cgtattttcg aatcggtaat cacgcgattg aatcgctcaa
    10021 cgggaccgat ggagtatgaa tgtacagcac caaatttcga gctgtcagtc aggactatca
    10081 cttcgcactc tttttccagc acggcattga ccacatcggt acgcatcata tcgcgaccgg
    10141 tgaatccagt ttcaggttgc cagccatcaa taccaataaa tgctttgctg aaatgcacct
    10201 gctggatgca ctggcgtgtc aacgggccaa ccatactttc gctttttttc tggtacacgc
    10261 caccgagcaa aatgacttca caaggcgcgt ctttcagcaa atgcgcgatg tagctgctga
    10321 ccgtgatgat agtgacattt ttcttctgct cccccagagt ccgggcgagc agggcattgc
    10381 tgctgccatt ttcgataaag atggtttcgc ccggttgaac cagtgacgcg gcaaactcgg
    10441 cgagttcacg cttcagcgta tagttgctca tcatacgggt ttcgacgtca tcactatcaa
    10501 gcgaaactgc aaagccatgt gcacggcgga ggtaactcag tttttcgagg gtgttgagat
    10561 cctggcgaat ggtaacttca gaaactccag tggcttttgc cagatcggtt acgctaacct
    10621 gaccctggtc aatgaccatc tgtagaatag tttgttgtcg ggaattcatc gctgtaattc
    10681 ttataacgtt ataatactaa ataaaaaaat actgacgcgc cgggcatcct caaataggaa
    10741 aacctgaagg cacgattgtt aaatctccca cggtgccttc gggatgtctg gcgtagattc
    10801 agcaacctgg cgcaagagtt ctgctttaag tatctcgaga ttatggatcg cagagtggta
    10861 atcgcctgcc acaaggatat ctaacacaat atcaatacgt tcggcaacgc gctgggcatc
    10921 aaattcagac atcaaggaca tccttatagc aaaagtggaa aatgaataat gcaaaaattg
    10981 ccgctaaaag agaagtgttt aacagcaacg gctaattatc atccaggaat acgatatata
    11041 atgacgggat atagcgctaa gtatatatat tcatctactt atgcgcgctt cagatagcgt
    11101 ttataccagc gttcgaaggc gacggcgggc atcggtttgg caaacaaaaa tccttgccgc
    11161 tcattgatcc cgttcttggt taaaaaagca tcttcctttg cactctctac accttcggcg
    11221 atcacctgaa gattcaatgc ctgggccaca gcgacgatcg cccggaccag tgactgcgag
    11281 acaggttgtt tgtgaatatc tcgaacaaaa acctggtcaa gtttgatggc atcgatcgga
    11341 aagcgcgcca gttgcgaaag tgaagagtag ccggtaccaa aatcatccag atgcacttgc
    11401 gcacctagtt ggctaaattg ttgaataaca gacagtgcca gttcatcatt ctcaatcaga
    11461 caactctctg tcagttcaac atctataggg cagtattcaa aattgagttc ctggagaacc
    11521 tgtttcaggg cggtgaaaat ggtttgatcg gcgagctgac gtgcagaaat atttaccgcc
    11581 actcgcaggt ttatgccttt atcccgccac tttgccacct ggcgtacgac atcgagaatc
    11641 acccaacggc ctaaaggcac aattagccct gactcttcgg cgtaggaaat gaagtccaac
    11701 ggtggaatca acccacgttc aggtgactgc caacgtacta gtgcttccag actgcgcact
    11761 tcgccacgcc aggtgatttt cggttgatag tgaataacca actgatcgtt ttccagtgct
    11821 ttacgcaagt tggtatccag ccagagatat tcaaataccc gttgattcat ttctggggta
    11881 aaaacgcaaa attgtcctcg tccgccttcc ttcgctgtgt acattgctgt gtcggcgtga
    11941 cgaataatag ccgtgctgtc tgaaccatgt tcgggagaga gtgcaatacc tactgaacag
    12001 ctggtataaa cttcaattaa accaatgcga aaggggagcc gtaagcgggt caaaattcgt
    12061 gatgccattg cttccagcgc gctttgtgag gtgttggatg ccagtaccag aaactcatcc
    12121 ccacctggac gcgccaacac ctggtcatgt tcgagacagc ttaaaatagc caatgacacg
    12181 tcgcgtaata actggtcacc aaacaaatgc ccataggcgt cgttgacctt tttgaaatta
    12241 tccaaatcaa gatacacaac cccaacttta ttgttatctg catgattaat agcgtgatcg
    12301 attaaatcct gcattgcgtt acgattcggc agtccggtga tactgtcggt atttgccaga
    12361 atacgcagtc gctcctgagc gcggcgctct tcggtaatgt cggtgccgga acagattaaa
    12421 aaaatctcgc ttttgccact gccgctgtgg acaaatttat tgcgaaacag aaacagccgc
    12481 tggcctttac atgttggtat ccacagttcg acttcatatg cattgccgct tcgaaaaaat
    12541 acacggttat tgcgcctgga tgccgcagct tcacgacggc tcataaacag tttaaacacg
    12601 ctttggccaa tgacgtcgtg ttctttcaac ccagtgtaat cttcacataa ccgattgaag
    12661 cgttggatat tcccccggct gtcgagaatc actattgcgg aatgtgcttc agaaactacc
    12721 tgctcagcaa aggaaagccc gtgtgataaa tcacgagcaa ctgccggtgt gtcatgccat
    12781 gcagacgcgt tgccagccca ttcccgttta ttgatttttc gtcctaccag atgcacagaa
    12841 agatcgctct catcgacggt cagactcatc atcaggctgg aggtgatgac cgtcatttcc
    12901 ctgatgcgag cagcctgttc ggcagataac tgcaaagtac gatcaggttc tgtggtttcg
    12961 gtggtagaaa agcgcaaaac atcgctgctt tccgtcaacc gccagtatgg attgtgcgat
    13021 ccgagaaagt tgtacaacgt tgtggactcc ctaacggttt tcatgatgca tatcctccgg
    13081 ttaaataaaa ggaacaacat
//
LOCUS       DAJTMC010000114        13098 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.114, whole genome shotgun sequence.
ACCESSION   DAJTMC010000114 DAJTMC010000000
VERSION     DAJTMC010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13098
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.114"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            4..1632
                     /locus_tag="OO130_002911"
     CDS             4..1632
                     /locus_tag="OO130_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001338794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HCR5366238.1"
                     /translation="MFTAIIAVCILFIIGIIFARLYRRASAEQAFVRTGLGGQKVVMS
                     GGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFFVRVKQSVEGIA
                     TAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENFVQGVQNTVAED
                     LSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLIQETERRRRERNEVEQDVEV
                     AVREKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNARIAAFEAERRREAEQTRI
                     LAERQIQETEIEREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKSIAIAAKSEQQS
                     QAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETKAVELTVRAKAE
                     KEAAEMQAAAIVKLAEATRKKGLAEAEAQRALNDAINVLSDEQTSLKFKLALLQALPA
                     VIEKSVEPMKSIDGIKIIQVDGLNRGSAAGDANTGNVGGGNLAEQALSAALSYRTQAP
                     LIDSLLNEIGVSGGSLAALTSPLTSTTPVAENVE"
     gene            1871..1930
                     /locus_tag="OO130_002912"
     CDS             1871..1930
                     /locus_tag="OO130_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCR5366239.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            2247..2306
                     /gene="ibsE"
                     /locus_tag="OO130_002913"
     CDS             2247..2306
                     /gene="ibsE"
                     /locus_tag="OO130_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HCR5366240.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(2426..3859)
                     /gene="hldE"
                     /locus_tag="OO130_002914"
     CDS             complement(2426..3859)
                     /gene="hldE"
                     /locus_tag="OO130_002914"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HCR5366241.1"
                     /translation="MKVTLSEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(3907..6747)
                     /gene="glnE"
                     /locus_tag="OO130_002915"
     CDS             complement(3907..6747)
                     /gene="glnE"
                     /locus_tag="OO130_002915"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HCR5366242.1"
                     /translation="MKPLSSPLQQYWQTVVERLPEPLAEKSLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQSPQADEWQHYAAWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSHLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSRSLLPTLSVIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNDREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFN
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMMLPREGKTLQTEVREMREKMRAHLGNKHRNRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERDLVRASWQKWLVEE"
     gene            complement(6770..8071)
                     /gene="ygiF"
                     /locus_tag="OO130_002916"
     CDS             complement(6770..8071)
                     /gene="ygiF"
                     /locus_tag="OO130_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HCR5366243.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRGHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVAVDDSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELALAQWQYHEELWVRGNDAAKEQVLAAIGLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTKTAMAKLALTEWLVSKAWQPFLDAKAQSKM
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLTRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            8313..8933
                     /locus_tag="OO130_002917"
     CDS             8313..8933
                     /locus_tag="OO130_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HCR5366244.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPTRKRKERWMN"
     gene            8997..10235
                     /gene="cca"
                     /locus_tag="OO130_002918"
     CDS             8997..10235
                     /gene="cca"
                     /locus_tag="OO130_002918"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HCR5366245.1"
                     /translation="MKIYLVGGAVRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(10392..11213)
                     /gene="bacA"
                     /locus_tag="OO130_002919"
     CDS             complement(10392..11213)
                     /gene="bacA"
                     /locus_tag="OO130_002919"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCR5366246.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(11304..11672)
                     /gene="folB"
                     /locus_tag="OO130_002920"
     CDS             complement(11304..11672)
                     /gene="folB"
                     /locus_tag="OO130_002920"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HCR5366247.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            11777..12394
                     /gene="plsY"
                     /locus_tag="OO130_002921"
     CDS             11777..12394
                     /gene="plsY"
                     /locus_tag="OO130_002921"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HCR5366248.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(12407..>13098)
                     /gene="ttdR"
                     /locus_tag="OO130_002922"
                     /gene_synonym="dan"
     CDS             complement(12407..>13098)
                     /gene="ttdR"
                     /locus_tag="OO130_002922"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
                     /protein_id="HCR5366249.1"
                     /translation="RLVDDVTQIKTRPEGMIRIGCSFGFGRSHIAPAITELMRNYPEL
                     QVHFELFDRQIDLVQDNIDLDIRINDEIPDYYIAHLLTKNKRILCAAPEYLQKYPQPQ
                     SLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVKVSGHLSSNSGEIVLQWALEGKGI
                     MLRSEWDVLPFLESGKLVQVLPEYAQSANIWAVYREPLYRSMKLRVCVEFLVAWCQQR
                     LGKPDEGYQVM"
BASE COUNT         3165 a         3682 c         3281 g         2970 t
ORIGIN      
        1 tggatgttta ccgcgattat tgccgtatgc attctgttta ttattggaat tattttcgct
       61 aggctctatc gtcgcgcttc ggcagagcaa gcttttgttc gtactggttt aggtgggcaa
      121 aaagtggtga tgagcggtgg cgcaatcgtg atgccgatct ttcatgaaat aatccccatc
      181 aatatgaata ctctgaagct ggaagtcagc cgctcaacca ttgatagcct gattacgaaa
      241 gatcgtatgc gtgtcgatgt agtagtcgct ttctttgtac gggtaaaaca gtctgtagaa
      301 ggcatcgcca ctgcggcgca gacgttgggg caacgcacgc tatcaccaga agacttacgt
      361 atgttggttg aagataaatt tgtcgatgcc ctccgtgcaa cagctgcaca aatgaccatg
      421 catgagttac aggatacccg cgagaacttc gtgcagggag tacaaaatac tgtcgcagaa
      481 gacctgtcga aaaacggtct tgagctggaa agcgtttcac ttaccaactt taaccagacc
      541 tcgaaagaac atttcaatcc gaacaatgcc tttgacgccg aaggtttaac caaactgatt
      601 caggagacgg agcgccgtcg tcgcgaacgt aacgaagttg aacaggatgt agaagttgcg
      661 gtgcgtgaga aaaaccgtga tgcactttcg cgcaagttgg agattgaaca acaagaagcg
      721 tttatgacgc ttgagcagga gcagcaggtt aaaacccgta ctgccgaaca gaatgcacgt
      781 attgcggctt ttgaagctga acgtcgtcgt gaagcagagc agacgcgaat tctggctgaa
      841 cgacaaattc aggaaacgga gatcgagcgc gagcaggccg tccgctcaag aaaggttgag
      901 gctgaacgtg aagttcgtat taaagagatc gaacagcagc aggtcaccga aatcgccaac
      961 cagacgaaat cgatcgctat tgccgccaaa tcggaacaac agtcccaggc agaagcgcgt
     1021 gctaaccttg cactcgcaga agcagtaagt gcgcaacaaa acgtagaaac cactcgccag
     1081 accgcagaag ccgatcgtgc taaacaagtt gccctaatcg ctgccgcgca ggatgcagaa
     1141 accaaagcgg ttgaactgac cgtgcgggcg aaagcagaaa aagaagccgc agagatgcag
     1201 gcggcggcta tcgttaagtt agccgaagct acacgtaaaa agggtctggc ggaagcagaa
     1261 gcacaacgtg cgctgaacga tgctatcaac gtactttctg atgaacaaac cagccttaaa
     1321 ttcaaactgg ccttgttgca ggcgctacct gcggtaatag aaaaatccgt tgagccgatg
     1381 aaatctattg acggtatcaa gattattcag gtcgatggtc tgaatcgtgg cagcgctgcg
     1441 ggtgatgcaa acacgggtaa tgtggggggc ggaaacctgg cggaacaagc attatcagcc
     1501 gctctctctt accgcacaca ggcaccgctg attgactcct tgctcaatga aattggcgtt
     1561 tcaggcggct cactggcggc attgacttca cccttaacct caacaactcc cgtcgccgaa
     1621 aacgtagaat aaattttcag ctatgaacgt ccctggcaca aaaagccggg gattttttat
     1681 atctgcgttc cgctaaaaac tgcaaatgat caggtcgttg caacgttttg cgtgaccgct
     1741 cggggaaggc aaaactgcct ctgggaaagc gttgctcgag gtccggcgct catcaacaat
     1801 cggggagtca gcaaagggct gaaacgggaa agcccctccc gaggaagggg ccttacagaa
     1861 ggaaagggtt atgatgaagc tcgtcatcat actgattgtg ttgttactcg taagtttcgc
     1921 agcttattaa cagccaatca gaggggggag aaatcctccc tcacccttgt tcctttactt
     1981 taggttgaaa aaaaatcagc gtcaagaagg tttcattaac acatatccct agcccaaaaa
     2041 gccggggatt ttttatatct gcgttccgct aaaaggtgca aatgctcagg acgttgcagc
     2101 gttttgcgtg accgctcggg gaaggcaaaa ctgcttcagg gaaagcgttg cgcggggtcc
     2161 ggcgctcatc aacaatcggg gagtcagcaa ggggctgaaa cgggaaagcc cctcccgagg
     2221 aaggggcctt atagaaggaa agggttatga tgaagttcgt catcatactg gttgtgttgt
     2281 tactgttaag tttcccgact tactaacaac tgatcagagg ggggagaaat cctcccttac
     2341 ccttgttcct ttactttagg ttgaaaaaac aacagcgtca ataggcctgc ctgctacgaa
     2401 gcgagatctg tgaaccgctt tccggttagc cttttttatc ctgttggatc ttcttgatga
     2461 tgttagtggt cgagcaaccg tcttcgaagt tgagcaccag cacttcgccg ccgttggccc
     2521 agacttcttt actcccggcg atctcttctg gtttatagtc gccgcccttc accagtaaat
     2581 ctggcaggat cccggcgatc aagcgctgcg gcgtgtcttc ttcaaacgac accacccagt
     2641 cgaccgcttc cagtgcgccc agcacaatca tacgctgttc gagcgggtta acggggcggg
     2701 aatccccttt cagccgtttg gtggaggcat cgctgttcac agcgacaatc agacggtcac
     2761 ccagtttgcg agcatttgcc agataggaga cgtgcccggc atgcaggatg tcaaagacac
     2821 cgttggtcat taccaccttt tcgccgcgtt tacgcgctgc cgctacggcc agcttcagtt
     2881 cctcttcggt catcacgcca aagccggtat ctgcgcgccc acgcactgca ttttccagct
     2941 cgatcggcga aaccgtggag gttcccagtt tgccgaccac cacgccagcc gccgcattgg
     3001 caaagaagca ggcttcttcc agcgaattac ccgctgccag cgttgccgcc aggacgccaa
     3061 tcaccgtgtc gcccgcaccg gtaacgtcat acacttcctg cgcctgggtt ggcatatgca
     3121 gcggcgcttt acccggttgc agcagcgaca taccctgttc ggaacgggtc actaacagag
     3181 ccgagagttc gtaatcggca atcagtttca tgccgcgctc aacaatctct tcttcggtct
     3241 tacatttacc gacaacagct tcaaattccg agagattcgg cgttaacagc gtagcgccgc
     3301 ggtagcgctc aaaatcggta ccttttggat caatcagcac cggaacgccc gctttacgcg
     3361 ccagttggat catctgctgt acgcttgcca gcgcaccttt ggcgtagtca gaaagcacca
     3421 gcgcgccaat cgaactcagc gcctgattaa tccgctcgtg cagcggctgc ggatcaacgc
     3481 cttcgaaacc ttcttcgaaa tccagacgga tcagctgttg gttgcgggaa agtacccgta
     3541 atttggtgat ggtcggatgc gtcggtacag aaacgaagtc gcatttgacg ttgacgtcgg
     3601 ccagagattt actcagcgcg cgcgctgcat cgtcaatgcc cgtcaacccg accaggcgtg
     3661 cattagcacc gagagaagcg atattcatcg ccacgttagc cgcgccgccc ggacgttctt
     3721 cgatggtatt cactttaacc acgggcaccg gcgcttccgg cgagatacgg ctggtgggac
     3781 cgtaccagta acgatccagc attacatcac caaccaccat cactcctgca cgttcaaact
     3841 ctgacagcgt tactttcatt cctgtctcct gagagattca aaatttgcgc gcgataatac
     3901 catacttcat tcttccacca gccacttctg ccagcttgcc cgcaccagat cacgctctgc
     3961 ggtgaagcaa tcctccgaca catggcccgg caattcctgt aatgccagat gatgaagttc
     4021 atcgcgcagc gtcgtgtaag cacgggtcag cgccatcgct tcctgctctt ccataatgtc
     4081 gttttgcgcc agcagctcca gaatacgcac gttgtctgac cagcgcgtta acttcggttt
     4141 ttcatgagcg tagcgcaaca ccagatattg ggtaataaat tcgatatcgg taattccgcc
     4201 ttcatcggct ttgatatcaa agcgattacg atgtttattg ccgagatgag cgcgcatttt
     4261 ctcgcgcatt tcccgcactt ccgtttgcag agttttacct tcacgcggca gcatcataat
     4321 ctcgcgacgc actgcgtcaa agtgcgcggt gagctgcgga tcgccgtaca ctacacgcgc
     4381 acgcaccagc gcctgatgct cccacgtcca ggcctcgttt ttctgataat cagcaaatgc
     4441 ttctgcggat gtcaccagca ttcccgccgc cccggacgga cgcagtcgtg catccacttc
     4501 atacaaaatg ccggaagagg ttcgcgtatt gaacagatgc ataatgcgtt gcgccagacg
     4561 cagataaaac tgtcgcccgt cgatttcccg ctcgccgtca gtcatcgcat ccattgggca
     4621 atcatggagg aagatcaggt caagatcgga actgtagcct aactcccagc cgcccagctt
     4681 gccgtagccg accaccgcaa aaccacggcc ttcgcgatcg ttgaggtgat ttggcttacc
     4741 gtagcgggcc accatttgaa cccacgcctg ctgaacgacg gcatctatca tagcctctgc
     4801 cagccaggtt aagtgatcgc tcactttcat caccggtagc gtaccagcga tatccgctgc
     4861 ggcgatgcgt aacagctgcg cctgtttgaa ctgacgcagc gcctcaagct gttgctcttc
     4921 gtcatcttcc ggcacgcgca gcaaatactg gcgcaactca tcgcggtagg catcggtcgc
     4981 cgtcggctgg taaagagtgt ttggatcgag caattcatcc agcaataatg gataacgcgc
     5041 cagctggctg gcaatcatcg gcgatgcggc acacagagaa atcaaatgtt taagcgccgc
     5101 agggaattca ctgagcaatt ctaaataggt ggtgcgggta acaatcccca ccagcaaggc
     5161 ggtaatgcgc gacagcgtaa cggcagcgtc ttcacgcgca cagacatcac ttagcagatg
     5221 tggcatcaga tggtcgagca cctgacgtcc tcgcggcccg atagtacgct tatccagctc
     5281 tttgcggaaa tcagcaatca gcgttaggac ctgtttgcga tcatcctcgc taagatgcgc
     5341 cagcactggc gtagtgtcat cttcctgcaa cgcatcctgc cacagctcac gccactgttc
     5401 cgacagcgac tcttcctgag tttcactttc gtcgtcgcca atcaattcat taaacacccg
     5461 gcgcacattg gtcatatgtg cggtcagcgc cccggtcagt tgcggccagt cagcaaagtc
     5521 catcgcccac gccagccgcg cacggttaag ctcatcagaa ggaagcgttt gggtttgttc
     5581 gtcgttaatg ctttgcagca ggttttccag acgccgcagg aacagatatg ccactcgcaa
     5641 ttgttcagca tcgttttcag aaagcagatg cagcgcggca atgacgctga gcgttggtag
     5701 taaagagcgc gactgcagcg acggttcgcg cccaccgcga atgagctgga acacctgaac
     5761 gataaattca atttcgcgaa tgccgcctgc gccgagtttg atattgtcgg tcaaaccacg
     5821 tcgacgcact tcacgggcaa tcatcccttt catgttgcgc agcgactgaa tcacgctgaa
     5881 atcgatgtaa cgacggaaaa caaacgggcg cagcatcgca cgcaactcgt tagcatagac
     5941 gccttcgcta tcgcccataa tccgcgcctt gaccatcgcg taacgctccc agtcgcgccc
     6001 ctgctcctgg taataatcct ccaacgcggc aaagctcagc accagcgggc cactttcgcc
     6061 aaacggacgc agccgcatat ccacgcgata gacgaagcca tcctgcgttg gttgatccaa
     6121 cactttaatc agccgctgcc ccatgcgggt aaaaaactgc gcgttatcca gttcccggcg
     6181 tccgccctgc gtacaaccat gttccggcca ggcaaaaatc agatcgatat ctgaggagaa
     6241 attcagttcc ccaccgccca gcttacccat gcctaaaatc agcagcggtt gcgcttcgcc
     6301 ctgcgcattg cacggcgttc cccactcgcg gcagcaagcg tcatacagcc agtcacgcgc
     6361 ggcgacaatc agcgtctccg ccagatggct gagctgctgc aatatactct cttcagtaac
     6421 caaagccagc gtttgcgccc aggcgatgcg caccataatg cgccgccgga atagccgcag
     6481 ctcgcgcatt aacccggctt cgtcactcac attgctgagc gcctcctgca accatgccgc
     6541 gtaatgctgc cattcatcag cctgcggcga ttggctttcc agttccgtca gccactctgg
     6601 atgcgcaatc acgctgtcct gcacaaaatc actgaaagta agtactgact tcgcctgtgc
     6661 gctaagtgat ttctcggcta aaggctctgg cagccgctca acaacggtct gccagtactg
     6721 ctgtaacggt gaagagagcg gcttcataag cgattttatc cttgcctgat taacgttttc
     6781 cgctgtgcaa ccagaacggt tcctgattgt ttgcctcatt acggaaatgt tcaatttcaa
     6841 tgcgttgccc ggtcgcaata gcgtgacgca gcccctgcca gttctccagc caggcttgcg
     6901 cgacgacagg atcatagtaa cccgccagca acagtattga gtcaatatca cgcgtcagac
     6961 gtggcaactg gtcacggtag cgatcgccca acggctggca gaaaacgctt ttcagttcag
     7021 cggcatggcg ggaaagatgg atatcggcaa agcgtttgaa ggaatcgctc attttactct
     7081 gcgctttggc atctaaaaac ggctgccagg ctttgcttac cagccattcg gtcaacgcca
     7141 gcttcgccat tgccgttttg gtagagtaga ccgccgtcac ggcagaaacc gcagaagcga
     7201 tggtcgcttc gcattgagtc agcaaatcgc gtaagtgagt gctcgctttg cgcggcacaa
     7261 taccaccgaa cagcatcagt gtatgacgga ccagaccaat ggctgccagc acctgttctt
     7321 tcgccgcatc gttgccgcgt acccacagtt cttcatgata ctgccattgc gctaacgcca
     7381 gctcgagcgc cgcttccagc ccctgttcca catcggcttt tgccgcaaca tgcaaaatgg
     7441 tggtcggttt gatttcacgc gccggattgc cctgcgccag atgatagcca cgcgccgctt
     7501 tgctcaggct gccctggcgt aatccggttt gcgataccag ttggttcgcc agtttcagca
     7561 ccgcgcgcgt gtcgccgcta agcagttcca gttccagctc acagataggt tcagcaaatt
     7621 cccccgcttt cacctccccc tgatcgagag cgatttcaat tcggctgtca tcgaccgcca
     7681 ccagccattt ttcgcgataa aaatcggtgc tgaacagcgg ttgtacgcgg gaagcgagat
     7741 cggcgggcaa ttcgccgttc ggccagactt ccgtcggtaa ctgcgccagg tcgagcgtcg
     7801 gttcgctcaa cgccacgtta tattccgggc gctggtgtaa gccgcctgtc actcttcctg
     7861 caactttcat ggtcatctca tagcgaccgt tttcgccacg aatacgtaag cccatatcgt
     7921 gcccacgcag ccagttatcc ggcgtttcgt agtaaatatt cagcaactgc acggggtcat
     7981 ggtgctcgcc gcccagcgta ttgagatggt cacgcaacgc ctcaacggca ctgtgattaa
     8041 caataaactt taattcgatt tcctgagcca tggcctttac ttatcggtta tgtcacataa
     8101 gtgacgatga acggcgaact taatgcgatc ttttttgtca gtagatagta ttttgcgcca
     8161 aattgccatg caacgggcaa tttgacgggc gtaaaagttt gaagcagtgg caagacctga
     8221 cacagaggat tccgattgat gtcgaatgct ttgcgtagcg acactgatgc cactactatc
     8281 gttccacttt ccatgacaat aacgacagcc tgatgccaaa attacgcctg atcggattaa
     8341 ctttactcgc acttagcgcc actgccgtct cacacgctga agaaacgcgc tatgtttctg
     8401 atgaactgaa tacctgggtc cgtagcggtc cgggagatca ttatcgcctc gtgggcacgg
     8461 ttaacgccgg cgaggaagtg accttattac aaactgacgc caacaccaat tatgcccagg
     8521 tgaaagacag ctctggccgt accgcctgga tcccgttgaa acaacttagc actgagccaa
     8581 gcctgcgctc ccgtgtgcca gatctggaaa atcaggtcaa aaccctgacc gataaactca
     8641 ccaatatcga taacacctgg aatcagcgca ccgcagaaat gcagcaaaaa gtggcgcaga
     8701 gcgacagcgt gatcaacgga ttaaaagaag aaaatcagaa attgaaaaac gagctgattg
     8761 tcgcgcagaa aaaggtcgat gccgccagcg tacagctgga tgacaaacag cgcaccatca
     8821 tcatgcaatg gtttatgtat ggcggtggcg tgctggggct tggcttgctg ctcggtctgg
     8881 tactgccaca cctgatccca acgcgcaaac gcaaagagcg ctggatgaac taaatcgcct
     8941 tcttcgttgc actgtcttat tattaggaat tgtgtatttg acgaagggat gatggcgtga
     9001 agatttatct ggtcggtggt gctgttcggg atgcattgtt agggctaccg gtcaaagaca
     9061 gagattgggt ggtggtcggc agtacgccac aggagatgct cgacgcgggc taccagcagg
     9121 taggccgcga ttttcctgtg tttctgcatc cgcaaacgca tgaagagtat gcgctggcac
     9181 gtaccgaacg gaaatccggt tccggttaca ccggttttac ttgctatgcc gcaccggatg
     9241 tcacgctgga agatgatctt aagcgtcgcg atctgaccat taatgcgctg gcccaggacg
     9301 ataacggcga gattatcgac ccgtacaacg gtctgggcga tctgcaaaat cgtctgttgc
     9361 gccatgtttc ccccgctttt ggcgaagatc cgttacgcgt tctgcgcgtg gcgcgttttg
     9421 ctgcgcgtta tgcccacctc ggttttcgta ttgccgatga aactctgacg ctgatgcgcg
     9481 agatgaccca tgcgggtgaa ctggaacacc tgacgcctga acgggtatgg aaagagacgg
     9541 aaagcgccct caccacccgc aatccccagg tgttcttcca ggtactgcgc gattgcggtg
     9601 cacttcgcgt tttattcccg gaaattgacg ccctgtttgg cgttccggcc cctgccaggt
     9661 ggcatccgga aatcgacacc ggtattcata ccttaatgac gctctcaatg gcggcgatgc
     9721 tgagtccgca ggttgatgtc cgtttcgcaa ctttatgcca cgatctcggt aaaggactga
     9781 cgccgccaga actttggccg cgtcatcatg ggcatggccc ggcgggtgtt aagttagtgg
     9841 aacaactatg ccagcgtctg cgcgtgccaa atgaaattcg cgatttagcc agactggtgg
     9901 ctgagtttca cgatctcatc cacaccttcc caatgctgaa cccaaaaacc atcgtcaaat
     9961 tatttgattc catcgatgcc tggcgtaaac cgcagcgtgt cgagcaactg gcgctgacca
    10021 gcgaggctga cgtacgcggc agaaccggtt ttgaatcagc ggactacccg caaggccgtt
    10081 ggttgcgcga agcctgggaa gtggcgcagt cagtgccgac aaaagcggtc gttgaagcgg
    10141 gatttaaagg tgtggagatt cgcgaggagc tgacccgacg gcggattgcg gcggtagcca
    10201 gttggaagga acaacgttgc ccaaagcctg actgagtata aaactaaacg ttaacacgaa
    10261 ccatcccctc gccccttcgg ggagagggtg aggggaacag accagcaccg gtgcgaacaa
    10321 tcacccccac cccggccctc tccctacaga gcgagggaga aaaccaggcc tgctcccgaa
    10381 ccttttctca atcaaaagaa cacgacgtac accgcagccg ccacaataaa gcgataaatg
    10441 gcgaacggga taaacgaaat gcgcttaatc aattgcagga aggttttaat cgctatcagc
    10501 gccaccacaa aagcggtgat aaacccaacg gcaaacatcg ggatatcgcc ggttgtcagg
    10561 aagccccagc ttttgtagag atcgagcgcc gttgcgccca tcatcatcgg taccgccagc
    10621 aggaacgaga actcggaagc agcgtaacgg ctcacgccca tcagcatccc acctgaaatg
    10681 gtggccccgg aacgggagaa acccggccac agcgccagac actggaaaca gccaatcata
    10741 aacgcctgac gataggtcat atcatcaaga cccggcgcac gcggctcttt cggcttcagg
    10801 cattcggcgg caatcagcaa caaaccgcca acgaccagtg catacatcac atttatcggg
    10861 ttaaacaatg acttaatcgt gtcgtggaac aacagcccca gtaccaccgc cggaatcatc
    10921 cccagcaaaa tgtggatcag cgttaaacga cctttgcttt ctccttcgtg ctgcaacggg
    10981 cggccaaagt ggatgccaat caggccaaac agacgccgcc agaacatcac tactaccgcc
    11041 agaattgatc ctaactggat cacaacttca aaggttttcg ccgtgtcgcc ctcgaacccc
    11101 aacaagtgac cgacaataat catatggccc gtgctggata ccggcagaaa ttctgtcaat
    11161 ccttcgacca cacccaatat tgccgctatc agcagcgagt gcatatcgct catcaataaa
    11221 cccctaaatt attaaaatgt accgcttgtc cggactactg cagtaagacc aggttataac
    11281 cgtttggttt aacagctgta aaattaatta ttttctttca gattattgcc acgctcaatg
    11341 attacaccaa cattcgccgc ccgcgccact gcgcctggct tgctgagttt gatgcgcacc
    11401 cacggcgagt tgaagcgtgc taacagcagc tccgccacct cttcagccac gcgttccacc
    11461 agcgcaaaac gcgccccctc gacgtggctg accaccgttt ctgcaatgtc agcgtaactg
    11521 aggcaatccg ccacatcatc acttttcgcc gctttacggt tatcccatgc catttcgata
    11581 tcgaacacta acttctgttc gatggtctgt tcccagtcgt aaacaccaat agtggtgatt
    11641 accgaaagtt gctctataaa tacaatatcc atcacgtcct gcctgctttt tggctaaccg
    11701 gataccactt ccggcgaaat gtgcgtatta tccacagatt catcgttgaa cacgaatttt
    11761 caaaacggaa cagcttatga gtgcaatcgc gcctggaatg atcctcatcg cgtacctctg
    11821 cggctccatt tccagtgcca ttctggtttg ccgcttgtgt gggctgcccg atccgcgaac
    11881 cagcggctcc ggcaatccag gcgcaaccaa tgtgttacgt atcggtggca agggagcagc
    11941 cgtagcagta ctgattttcg acgttctgaa aggaatgttg cccgtctggg gcgcgtatga
    12001 attaggtgtc agccccttct ggctaggctt aattgccatc gccgcctgtc ttggacacat
    12061 ctggcccgtt ttcttcggat ttaaaggagg aaaaggcgtt gctaccgctt ttggtgccat
    12121 cgcacccatt ggctgggatc tcaccggagt aatggcggga acctggttac tgaccgtgct
    12181 attgagcgga tactcgtcgc tgggagcgat tgtcagtgca ctgattgctc cgttttatgt
    12241 ctggtggttt aagccacaat tcaccttccc agtttcgatg ctctcttgcc tgatcctgct
    12301 gcgtcatcat gacaacatcc agcgtctgtg gcgtcgtcag gagacaaaaa tctggacgaa
    12361 attcaaaaga aagcgcgaaa aggatcccga gtgatttctg gtggatctac atgacctgat
    12421 agccttcatc gggcttgccc agccgttgct ggcaccatgc caccagaaat tccacgcaga
    12481 cacgtaattt catgctgcga tagagcggct cccggtaaac agcccagata ttggcgcttt
    12541 gtgcatactc cggcaatact tgcaccagtt tgccactctc cagaaacggc aacacatccc
    12601 actcggaacg cagcataatc cctttgccct ccagcgccca ttgcaacact atctcgccgc
    12661 tattggagga aagatgcccg ctaaccttca ccgatttttt ctcctgaccg ttccccaact
    12721 cccatattcc gtgggtcata tcgcgctctt tggtcaccag acagtcatga cgacttaatt
    12781 cctgtaatga ttgtggctgc ggatattttt gcagatactc aggtgctgcg cataatattc
    12841 ttttattttt cgttaacaga tgcgcaatat aataatcagg aatttcgtca ttaatacgaa
    12901 tatccagatc aatattatcc tgcactaaat caatttgccg atcgaacaat tcaaaatgca
    12961 cctgtaactc aggataattg cgcataagtt cggtaatggc tggcgcaata tggctgcgcc
    13021 caaaaccaaa actgcaacca atacgaatca tcccctccgg gcgcgttttg atttgcgtga
    13081 cgtcatcgac cagtcgct
//
LOCUS       DAJTMC010000115        12952 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.115, whole genome shotgun sequence.
ACCESSION   DAJTMC010000115 DAJTMC010000000
VERSION     DAJTMC010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12952
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.115"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(234..1574)
                     /gene="fadL"
                     /locus_tag="OO130_002923"
     CDS             complement(234..1574)
                     /gene="fadL"
                     /locus_tag="OO130_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HCR5366250.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGNTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            1946..2230
                     /locus_tag="OO130_002924"
     CDS             1946..2230
                     /locus_tag="OO130_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HCR5366251.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            2410..3720
                     /gene="fadI"
                     /locus_tag="OO130_002925"
     CDS             2410..3720
                     /gene="fadI"
                     /locus_tag="OO130_002925"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HCR5366252.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLFGPAWSTPLALERAGLTM
                     SDLTLIDMHEAFAAQTLANIQLLGSERFACEVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGAWMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            3720..5864
                     /gene="fadJ"
                     /locus_tag="OO130_002926"
     CDS             3720..5864
                     /gene="fadJ"
                     /locus_tag="OO130_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HCR5366253.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPDEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIGNCKTVQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTTLEMILTGKQLRAKQALKLGLVDDVVPHSILLEVAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHATRPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTKGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            6067..6552
                     /gene="sixA"
                     /locus_tag="OO130_002927"
     CDS             6067..6552
                     /gene="sixA"
                     /locus_tag="OO130_002927"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HCR5366254.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            7227..7487
                     /locus_tag="OO130_002928"
     CDS             7227..7487
                     /locus_tag="OO130_002928"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5366255.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDT"
     gene            7494..7790
                     /locus_tag="OO130_002929"
     CDS             7494..7790
                     /locus_tag="OO130_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5366256.1"
                     /translation="MTTTFTGTVSSANSGNYYTIFNTDTGAAFNNVSLAIGDSLGTSY
                     KSGMGIDQKIVKDTSTNKGKAKQTLNFKAWLVGAADAPDLGNFEANTTFQITYL"
     gene            7872..10514
                     /gene="yfcU"
                     /locus_tag="OO130_002930"
     CDS             7872..10514
                     /gene="yfcU"
                     /locus_tag="OO130_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
                     /protein_id="HCR5366257.1"
                     /translation="MPDHSLFRLRILPWCIALAMSGSYSSVWAEDDIQFDSRFLELKG
                     DTKIDLKRFSSQGYVEPGKYNLQVQLNKQPLAEEYDIYWYAGEDDASKSYACLTPELV
                     AQFGLKEDVAKNLQWSHDAKCLKSGQLEGMEIKADLSQSALVISLPQAYLEYTYPDWD
                     PPSRWDDGISGIVADYSINAQTRHEENGGDDSNEISGNGTVGVNLGPWRMRADWQTNY
                     QHTRSNDDDEFSGDETQKKWEWSRYYAWRALPSLKAKLALGEDYLRSDIFDGFNYVGG
                     SVSTDDQMLPPNLRGYAPDISGVAHTTAKVTVSQMGRVIYETQVPAGPFRIQDLGDSV
                     SGTLHIRIEEQNGQVQEYDISTASMPYLTRPGQIRYKIMMGRPQEWGHHVEGEFFSGA
                     EASWGIANGWSLYGGALGDENYQSAALGVGRDLSTFGAVAFDVTHSHTKLDKDTAYGK
                     GSLDGNSFRVSYSKDFDQLNSRVTFAGYRFSEENFMTMSEYLDASDSGMVRTGNDKEM
                     YTATYNQNFRDAGVSVYLNYTRHTYWDREEQINYNIMLSHYFNMGSIRNVSISMTGYR
                     YEYDNQADKGMYISLSMPWGDNSTVSYNGNYGSGTDSSQVGYFSRVDDATHYQLNVGT
                     SDKHTSVDGYYSHDGSLAQVDLSANYHEGQYTSAGLSLQGGATLTAHGGALHRTQNMG
                     GTRLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNRLPENAEAT
                     QSVVQATLTEGAIGYRKFAVISGQKAMAVLRLSDGSHPPFGAEVKNDNEQTVGLVDDD
                     GNVYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPITSPAV
                     KPAIQEQTQRVTPTEPPTSISVNQ"
     gene            10534..11286
                     /locus_tag="OO130_002931"
     CDS             10534..11286
                     /locus_tag="OO130_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000482750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCR5366258.1"
                     /translation="MFNLTNTAKIVVPALALLATAVSFSSHASVTPDRTRLVFNESDK
                     SISVTLRNNDPTLPYLAQSWIEDAKGNKISSPLAVLPPVQRIDAMMNGQVKVQGLPDI
                     NKLPADRESVFYFNVREIPPKSNKPNTLQIALQTRIKLFWRPKALENTSMKSPWQHKV
                     TLIRNGQDFTVNNPTPYYVIISNASAKKGGNPPAGFTPLMMEPKTSAPLNAKMDSVPV
                     LTYINDYGARMPLYFKCTGNSCQVDEEQSRKG"
     gene            11302..11811
                     /locus_tag="OO130_002932"
     CDS             11302..11811
                     /locus_tag="OO130_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001733155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5366259.1"
                     /translation="MSHKFVAGAGLLVLAGWGISGLAVAGSDIKQVDLTLRILVDGPP
                     PCSVKGSAVKFGNVIINRIDGTNYQQDAKYTLNCGNKVSDDLRMQLKGNTAVINGETV
                     LSTDIAGLGVRIENAADNSLFAVGENNWTPFNVNTQPQLKAVPVKQSGVQLTPSEFNA
                     TMTMVVDYQ"
     gene            11808..12296
                     /locus_tag="OO130_002933"
     CDS             11808..12296
                     /locus_tag="OO130_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5366260.1"
                     /translation="MKKFLLLLLCAGCGAVLAAEDDITFHGTLMSPPACSISGGQTIE
                     VQFRDLIIDSIDGNYGRQKVDYELSCDSDVRDPEWNMTLSWTGTATSYNDSAIETDVP
                     GFGIELQHDGQRFKLNEPLSINATDFSQKPKLEAVPVKAADAVLTDTTFSAYATLRVD
                     YQ"
     gene            12293..12832
                     /locus_tag="OO130_002934"
                     /pseudo
     CDS             12293..12832
                     /locus_tag="OO130_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416836.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
BASE COUNT         3258 a         3089 c         3441 g         3164 t
ORIGIN      
        1 gtatcctgga aatgcagaaa agtaaactct ttgagaagga cgagcagaac gcctgaaaat
       61 atgcctttcc cttcaggttt gtctgtggat accgcttatt gatttgaaag ggcaagctgc
      121 caacaggctg tttgcaaaac tcaggaggta agcaatgagg acactacttt cggtgaagtg
      181 ggggagttaa agtcacctgc tatgcaggtg actttatcca ggcgaacgcg ttatcagaac
      241 gcgtagttaa agttcgtacc gaacagccag gctttacctt cagactcgaa ctggtatggg
      301 ccttcgttaa ttttcacgct ctgaccgtgc atataagaaa caccaacgtc gactgaagca
      361 tctttattaa atgcgtaagt cgtacctgca ctcagccaga aacggtcctg gtccggaatg
      421 gagatagaac gattctgagc cggaactggg ctgtcatcaa aggcgatacc ggtacggaag
      481 gtccagttat catcgtagta ataagtggta cccaacgcga tgcggtaagc atctttaaag
      541 ccttcatgtt tctggaacag cgtgttgcca ctggttgagg tcgctttcag ctgctggaac
      601 tgactccagc tggtgtaagc caggctatag tgaatcgccc actgtggatc aacacggtta
      661 taacctgaca cttcccacat ttcaggcagg ttcagcgtca gataacccga ttgcgttgcg
      721 ccacctgtcg cggtaggaat tggcaaaccg tagttattaa acgcacgatt aagatcgctg
      781 ctgtagttac ctttgaagtc aattttcact tcagaacggt aggtcagtgc atagcggtta
      841 tttttatcca gttcatacag gattccggcg ttccagccaa agccccactg gttaccgttc
      901 agatgagcga ttttggtatt actgtcgata ccgttggcgg tagctgccaa tgcttgccct
      961 tgaggagttt gcccggcagg agattgcata atctgaccag caaccaactg ccccagatcg
     1021 cctgcgaaac gttcaatttt cgcgcgagcg tagacggcgt tgaaaccaag accaaagctc
     1081 catgcattat ttaagcgata cgcaccgctt aagttcaggt tcatggtttc aaggtcagtt
     1141 gtacccccga cagagccgcc tgcataagta tcattaaact ctgttgccag accatagtta
     1201 gaggtaatag aagcgcccca accaaattgg tcgttaatcg gtgcaacaaa gtgcatgttc
     1261 ggaacccatg ccgtaggcgc gatgttatcg gctttcaggc tacgaccaga cggagacgtt
     1321 ccgctgatat tcacatccgg gtcaatataa accgcacccg cagaaaatgt cgggcggtca
     1381 aacatggtaa tcaatgcggg gttacggcta acgttacctg catcatcggc aattgcgcct
     1441 tcccctgaat aagcccggcc caggccagag gaagaaaatt cgtttaactg aaagcctgcc
     1501 gaccaggcct gggtggagat aagtgccact gcgactgcga gagcagactt tgtaaacagg
     1561 gttttctggc tcatgaccat aacctcaatg atttattttt atacaaacta tgttacgtgc
     1621 tgtaacagga gcgcgaagtg tagggtctga ggtacatctt agaaatcaga ccagtggcga
     1681 gagtataggt cggaccagct ggaatgttgc aagtatgttt ccatcttttt tcaaatatga
     1741 tctaagaaac gcgatccagc tggcaaaatt tggaggttgc gcactcgcgg attcgggtga
     1801 attttgtttt agatcatttt taagtgtgat ttcggtcact tatccgattt tgcagaatta
     1861 acaactggag cagcactttc cggaagcgta aaatatccac aacgtttatc tggcaccttg
     1921 ggtgcacata cagaggaaat caacgatgag taaatgtagt gctgatgaaa ccccggtttg
     1981 ctgctgtatg gatgttggca ccattatgga caactccgac tgcaccgcgt cttacagccg
     2041 cgtgtttgca aaccgcgcag aagcagaaca aacgctggcg gcgttgactg aaaaagcccg
     2101 tagcgtggaa tccgaaccgt gcaaaatctc cccgactttc actgaagagt ctgacggtgt
     2161 gcgcctggat atcgatttca cttttgcctg cgaagctgaa atgctgatct tccagctggg
     2221 tctgcgttaa ttcctctcgg gtacgttcgc ccctgtcagt tgctggcagg ggcgttttct
     2281 tttctttctc ctgtcttcac tgtgtcatag ctcccacttt cctttttctc cgcttggcta
     2341 aatgtaaaaa aatggttaag actatgatca ggtcagacca ctttatttat ttttttacag
     2401 gggagtgtta tgggtcaggt tttaccgctg gttacccgcc agggcgatcg tatcgccatt
     2461 gttagcggtt tacgtacgcc ttttgcccgc caggcgacgg cttttcatgg cattcccgcg
     2521 gttgatttag ggaagatggt ggtaggcgaa ctgctggcac gcagcgagat ccccgctgaa
     2581 gtgattgaac agctggtctt tggtcaggtc gtacaaatgc ctgaagcccc caacattgcg
     2641 cgtgaaattg ttctcggtac cggaatgaat gtgcataccg atgcttacag cgtcagccgc
     2701 gcttgcgcta ccagtttcca ggcggttgca aacgtcgcag aaagcctgat ggcgggaact
     2761 atccgagcgg ggattgccgg tggggcagat tcctcttccg tattgccaat tggcgtcagt
     2821 aaaaaactgg cgcgcgtgct ggttgatgtc aacaaagctc gaaccatgag ccagcgcctg
     2881 aaactcttct ctcgcctgcg tttgcgcgat ttaatgcccg taccgcctgc agtagcagaa
     2941 tattctaccg gcttgcggat gggtgacacc gcagagcaaa tggcgaaaac ctacggcatc
     3001 acccgagaac agcaagatgc attagcgcac cgttcgcatc agcgtgccgc tcaggcatgg
     3061 tcagacggaa aactcaaaga agaggtgatg actgccttta tccctcctta taaacaaccg
     3121 cttgtcgaag acaacaatat tcgcggtaat tcctcgcttg ccgattacgc aaagctgcgc
     3181 ccggcgtttg atcgcaaaca cggaacggta acggcggcaa acagtacgcc gctgaccgat
     3241 ggcgcggcag cggtgatcct gatgactgaa tcccgggcga aagaattagg gctggtgccg
     3301 ctggggtatc tgcgcagcta cgcatttact gcgattgatg tctggcagga catgttgttc
     3361 ggtccggcct ggtcaacacc gctggcgctg gaacgtgccg gtttgacaat gagcgatctg
     3421 acattgatcg atatgcacga agcctttgca gctcagacac tggcgaatat tcagttgctg
     3481 ggtagtgaac gttttgcttg tgaagtactg gggcgtgcac atgccactgg cgaagtggac
     3541 gatagcaaat ttaacgtgct tggcggttcg attgcttacg ggcatccctt cgcggcgacc
     3601 ggcgcgtgga tgattaccca gacattgcat gaacttcgcc gtcgcggcgg tggatttggt
     3661 ttagttaccg cctgtgctgc cggtgggctt ggcgcggcaa tggttctgga ggcggaataa
     3721 tggaaatggc atcagcgttt actcttaatg ttcgtctgga caacattgct atcatcacca
     3781 tcgacgtacc ggatgagaaa atgaataccc tgaaggcgga atttgcctcg caggtgcgcg
     3841 ccattattaa gcaactccgt gaaaacaaag agttgcgcgg cgtggtgttt atctccgcga
     3901 aaccggacaa ttttatcgcc ggagcagaca tcaacatgat cggcaactgc aaaacggtgc
     3961 aagaagcgga agctctggcg cggcagggcc aacagttgat ggcggagatt catgctttgc
     4021 ccattccggt tatcgcggct attcatggcg cttgcctggg tggtgggctg gagttggcgc
     4081 tggcgtgcca cggtcgcgtt tgtactgacg atcctaaaac ggtgctcggt ttgcctgaag
     4141 tacaacttgg attgttaccc ggttcaggcg gcacccagcg tttaccgcgt ctgataggcg
     4201 tcagcacaac attagagatg atcctcaccg gaaaacaact tcgggcgaaa caggcattaa
     4261 agctggggct ggtggatgac gttgttccgc actccatttt gctggaagtc gctgttgagc
     4321 tggcgaagaa ggatcgccca tcttcccgcc ctctacctgt acgcgagcgt attctggcgg
     4381 ggccgttagg tcgtgcgctg ctgttcaaaa tggtcggcaa gaaaacagaa cacaaaactc
     4441 aaggcaatta tccggcgaca gaacgcatcc tggaggttgt tgaaacggga ttagcgcagg
     4501 gcaccagcag cggttatgac gccgaagctc gggcgtttgg cgaactggcg atgacgccac
     4561 aatcgcaggc gctgcgtagt atcttttttg ccagtacgga cgtgaagaaa gatcccggta
     4621 gtgatgcacc gcctgcgcca ttaaacagcg tgggaatttt aggtggtggc ttgatgggcg
     4681 gcggtattgc ttatgtcact gcttgtaaag cggggcttcc ggtcagaatt aaagatatca
     4741 acccgcaggg cataaatcat gcgctgaagt acagttggga tcagctggag ggcaaagttc
     4801 gccgtcgtca tctcaaagcc agcgaacgtg acaaacagct ggcattaatc tccggaacga
     4861 cggactatcg cggctttgcc catcgcgatc tgattattga agcggtgttt gaaaatctcg
     4921 aattgaaaca acagatggtg gcggaagttg agcaaaattg cgccgctcat accatctttg
     4981 cttcgaatac gtcatcttta ccgattggtg atatcgccgc tcacgccacg cgacctgagc
     5041 aagttatcgg cctgcatttc ttcagtccgg tggaaaaaat gccgctggtg gagattattc
     5101 ctcatgcggg tacatcagcg caaaccatcg ccactacggt aaaactggcg aaaaaacagg
     5161 gcaaaacgcc cattgtcgtg cgtgacaaag ccgggtttta cgtcaatcgc atcttagcgc
     5221 cttacattaa tgaagctatc cgcatgctga ccaaaggtga acgggtagag cacattgatg
     5281 ccgcgctagt gaaatttggt tttccggtag gtccaatcca acttttggat gaggtaggaa
     5341 tcgacaccgg gactaaaatt attcctgtac tggaagccgc ttatggagaa cgttttagcg
     5401 cgcctgcaaa tgttgtttct tcaattttga acgacgatcg caaaggcaga aaaaatggtc
     5461 ggggtttcta tctttatggt cagaaagggc gtaaaagcaa aaaacaggtc gatcccgcca
     5521 tttacccgct cattggcgca caagggcagg ggcgactctc cgcaccgcag gttgctgaac
     5581 ggtgtgtgat gttgatgctg aatgaagcag tacgttgtgt tgatgagcag gttatccgta
     5641 gcgtgcgtga cggggatatt ggtgcggtat ttggcattgg ttttccgcca tttctcggtg
     5701 gaccgttccg ctatatcgat tctctcggcg cgggcgaagt ggttgcaata atgcaacgac
     5761 tggccacgca gtatggttcc cgttttaccc cttgcgagcg tttggtcgag atgggcgcgc
     5821 gtggggaaag tttttggaaa acaactgcaa ctgacctgca ataagaaggt caaagctata
     5881 tgaatccgcg ctgaatggcg gagtgttggt caaaatgtaa acgcatattg actatactta
     5941 cgccattgag gtaaaaaaca gcgtttcatt cggtgaatgg ataaggcaca atgccggcca
     6001 ccgttttctt tctctggttt cagatgaaag aaaatgggcg aatctggtta acaaaagcgg
     6061 tgcaatatgc aagtttttat catgcgtcac ggcgacgcag ccctcgatgc cgccagtgat
     6121 tcggttcgtc ctctgaccac taatggttgt gacgaatctc gcctgatggc gaactggctg
     6181 aaaggtcaaa aagtggaaat cgaacgcgtt ctggtgagcc cgttcctgcg agccgagcaa
     6241 acactggaag aagtagggga ttgtctgaac ctgccttcca gtgcagaagt tctgccggag
     6301 ttaacgccct gcggcgatgt tggtctggtc agtgcctatt tgcaggcgtt gaccaatgaa
     6361 ggtgttgctt cggtactggt gatctctcac ttaccgttag tcgggtatct ggttgccgag
     6421 ttatgtccgg gcgaaacgcc gcctatgttt accacctcgg ccattgccag cgtaacgctt
     6481 gatgaaagcg gaaacggtac attcaactgg cagatgagcc cgtgtaatct gaagatggca
     6541 aaagctatct gatgtgttcc aaattatgcc caatgaggta gattcattgg gcataattgc
     6601 tcatcaggca gcggatatct gtcaacctca ctactatcga agactctcct tatctataaa
     6661 taaaacactt tctacattca ttcggaaatt attcttctgc acgtatcttg attttgccca
     6721 aagttacatg ttgaaatgaa taatgtctgt ttgacactca ttgtcattta ttaatacaga
     6781 taatagagtt gaataatcag gcaggatgta attgaaactt gattaaattc tggctatagt
     6841 gtttatatat tatattgtca aaagtggata aatgccaatc gcattatata ttcattgttt
     6901 gttttttaag ctgttgatat tcattattga tatactttct attttttcta aatacttatt
     6961 ccgtattttt taaggatgtc cttaagataa tctttcttct gtcaatatat taattaaatc
     7021 tacatttact ctttagtaaa attaaattgc atcaggggtg tggaaacatc actatgcggc
     7081 cccacaccaa cgaatacaat tttctacgac ctgttctttt tggatcgcat gccgtattca
     7141 atggctgggt ataaacattg gctattgact gaatcaatgt attgcaatgt ttatattttt
     7201 aattatttat agtttaagga caaataatga gtaagtttgt taaaacagct attgctgcgg
     7261 caatggtgat gggcgtgttc acctctacgg caacaatcgc tgcgggtaac aacggtactg
     7321 cacgttttta cggcaccatt gaagactcag tgtgttctat cgttccggac gatcacaaac
     7381 tggaagttga tatgggtgat atcggtgccg aaaaactgaa aaataacggc accaccacgc
     7441 cgaaaagttt ccagattcgt ctgcaagatt gcgtatttga tacttaggaa acaatgacca
     7501 ctacctttac tggtaccgtt tcttctgcaa atagcggcaa ttattacacc attttcaata
     7561 ccgataccgg tgcggcattt aacaatgtta gcctggcgat cggtgactct ctgggtacct
     7621 cttacaaaag cggcatgggt attgaccaga aaatagtgaa agacacttct actaataaag
     7681 gcaaagcgaa gcagacactg aactttaaag cctggctggt cggcgcagct gatgcgccag
     7741 atctgggtaa tttcgaagca aataccacct tccagattac ttacctgtaa ggcatacccc
     7801 catagcgaca ctgctatggg ggatttaaat ggattttgac cgaacaagaa taaaaacgga
     7861 tagaaacgtg tatgcctgac cattctcttt ttcgattacg gattcttccg tggtgcattg
     7921 cgctggcaat gtcagggagt tatagcagtg tctgggctga agacgacatt cagtttgatt
     7981 cccgttttct ggaattaaaa ggcgacacaa aaattgatct gaagcgtttt tccagccagg
     8041 gatatgttga gcccggaaaa tacaatttac aggttcaact aaataaacag ccattggcgg
     8101 aagagtacga tatttactgg tatgctggtg aagatgacgc gagcaaaagc tatgcttgtc
     8161 tgacaccgga actggtagcg cagtttggtt taaaagaaga cgtggcgaaa aatctgcaat
     8221 ggagccacga tgctaaatgc ctgaaatccg gtcaactgga aggcatggaa attaaggctg
     8281 atttaagcca gtccgcatta gtcatttcac tgccacaggc ttacctcgaa tatacttatc
     8341 ccgactggga tccgccttca cgttgggatg acggcatctc cgggatcgtc gcggactaca
     8401 gcatcaacgc acaaacccgg cacgaagaaa atggcggtga tgatagtaac gagatcagcg
     8461 gcaacgggac ggtcggggtt aacctggggc cgtggcgtat gcgtgctgac tggcagacta
     8521 actatcaaca tactcgcagt aatgatgacg atgaattcag cggcgatgaa actcaaaaaa
     8581 aatgggagtg gagtcgctac tatgcctggc gggcgttacc atcattaaaa gccaaactgg
     8641 cgctgggcga ggattacctc agatccgata tttttgatgg ttttaactat gttggtggta
     8701 gtgtcagtac tgacgatcaa atgttgcctc ccaatctgcg cggctacgcg ccagacattt
     8761 ccggcgtggc acacaccaca gcaaaagtga ccgtcagcca gatggggcgt gtgatttacg
     8821 aaacgcaggt tccggctgga ccgtttcgta ttcaggatct tggtgattct gtctccggta
     8881 cgttgcatat tcgcattgaa gaacagaacg gccaggtgca ggaatatgac atcagcaccg
     8941 cctcgatgcc atacctcact cgtccaggtc agattcgcta taagatcatg atgggccgtc
     9001 cgcaagagtg gggacaccat gtcgagggtg aatttttttc tggtgctgaa gcttcctggg
     9061 ggatcgctaa cggctggtcg ttatatggcg gcgcactggg agatgaaaac tatcagtctg
     9121 cggcgcttgg cgtcggtcgc gatttgtcta cattcggcgc ggtcgcgttt gatgttactc
     9181 actcgcacac caaactggat aaagacaccg cttatggcaa aggttcgctg gacggtaact
     9241 ccttccgtgt gagttattcc aaagactttg accagctcaa cagccgcgtt acttttgctg
     9301 gatatcgctt ctcggaagag aactttatga ccatgagcga gtatctggat gccagtgaca
     9361 gcggaatggt acgcacgggc aacgacaaag agatgtacac cgccacttat aaccagaact
     9421 tccgcgatgc gggtgtttcg gtttatctca actatacccg ccatacctac tgggatcgcg
     9481 aggagcagat aaactacaac atcatgctct cgcactattt caatatgggc agtattcgta
     9541 atgtcagcat ctcgatgact ggctaccgtt acgagtatga caaccaggcc gacaaaggca
     9601 tgtacatttc gctcagtatg ccgtggggcg acaacagtac cgttagctat aacggtaact
     9661 atggcagtgg gacggacagc agtcaggtcg gttatttcag ccgtgtcgat gacgcgactc
     9721 actatcagtt gaacgtcggc accagtgaca aacacaccag cgttgacggc tattacagcc
     9781 atgatggttc gctggcgcag gttgacctca gtgcgaacta ccatgaaggg caatacacct
     9841 ctgcgggctt gtcgttacag ggcggcgcaa cgcttactgc ccacggtggc gcacttcacc
     9901 gtacccagaa tatgggcggg acacgcttgt tgattgatgc cgatggcgtt gccgatgttc
     9961 cggtggaagg taacggggct gctgtttata ccaatatgtt tggtaaagcc gtcgtttctg
    10021 acgtcaataa ctattaccgc aatcaggcgt atatcgacct caacagattg cctgaaaacg
    10081 ctgaagcaac ccagtcggtg gtgcaagcca cgctaactga aggtgccatt ggctaccgta
    10141 aatttgctgt cattagcgga caaaaagcga tggcggtgct gcgcctgagc gacggcagcc
    10201 atcctccgtt tggcgcagaa gtaaaaaatg ataacgagca gacagtgggc cttgtcgatg
    10261 atgacggcaa tgtttatctg gctggggtga aacctggcga acacatgagt gtgttctgga
    10321 gtggtgttgc gcattgcgat atcaacctgc cggacccgct gcctgccgat ctgtttaacg
    10381 gcttgttact gccatgccag cataaaggca atgtagcacc tatcacttcg ccggcggtca
    10441 aaccggcgat tcaggaacag acacagcggg tgacgccaac ggaacctccg acttcaattt
    10501 cagtaaacca gtaacgtgat taaggaatga tccatgttta atctgactaa cactgcaaaa
    10561 atcgttgtcc cggcactggc gctgctggcg acagcggtca gtttctccag ccacgcttcg
    10621 gtgacgcccg acaggacacg gctggtgttt aacgaaagcg acaagtccat cagcgtcacg
    10681 ctgcgcaaca acgatcccac actgccttat ctggcgcaga gctggataga ggatgcaaag
    10741 ggcaacaaaa tcagctctcc gcttgcggtg cttccgccag tgcagcgcat cgatgcgatg
    10801 atgaacggcc aggtgaaggt gcaggggctg ccggatatca acaaactgcc ggccgaccgc
    10861 gagagcgtgt tctatttcaa cgtgcgcgaa attccgccga aatcaaataa accgaacaca
    10921 ctgcagattg cgctgcagac gcgtatcaaa ctgttctggc gaccgaaggc gctggagaac
    10981 accagcatga aaagtccgtg gcagcataag gtgacgctca tccgtaacgg ccaggacttc
    11041 accgtcaata acccgacgcc gtactacgtg atcatcagca acgccagcgc taaaaaaggc
    11101 ggcaacccgc cggcgggatt cacaccgctg atgatggagc cgaaaacctc cgcaccgctg
    11161 aatgcgaaga tggacagcgt gccggtgctg acttacatca acgactacgg cgcgcggatg
    11221 ccgctgtact tcaaatgcac cggcaacagc tgccaggtgg atgaagagca gagccgcaaa
    11281 ggctgatgcc gggagggact tatgagtcac aagtttgtag cgggcgcagg gttgctggtg
    11341 ctggccggat ggggcatcag cggtttggct gttgctggca gtgatatcaa acaggtggat
    11401 ctgacgctgc gaatcctagt cgatggaccg ccgccttgct ccgtgaaggg aagcgctgtg
    11461 aaatttggca atgtgataat caacaggatc gatggtacca attaccaaca ggatgcgaaa
    11521 tacaccctca actgcggtaa taaagtcagc gatgacttac ggatgcagct caaaggcaat
    11581 accgccgtga tcaacggaga aacggttctc agcacggata tcgccggcct gggggtgcgg
    11641 atagaaaacg ccgccgacaa ctccctgttt gcagttggtg aaaataactg gacgcctttt
    11701 aatgtcaaca ctcagcctca gctgaaagcg gtgccggtaa aacagagcgg ggtgcaactg
    11761 actccgtcgg agtttaacgc cacgatgacc atggtggtgg attaccaatg aaaaaattcc
    11821 tgctcctttt actgtgcgct ggttgcgggg ctgtgctggc ggcggaagat gacatcacat
    11881 tccacggtac gctgatgtcg ccgcccgcct gtagcatcag cggcgggcaa accatcgaag
    11941 tgcaatttcg cgatctcatt atcgacagca ttgatggcaa ttatggtcgt cagaaagtgg
    12001 actacgaact gagctgcgac tcggatgtcc gcgatcccga gtggaatatg acactctcct
    12061 ggacggggac tgccacctcc tacaacgact ctgctattga gacggatgta ccgggcttcg
    12121 gaattgagct tcagcatgac ggtcagcgtt ttaagctcaa cgagccgctt tccattaacg
    12181 ccaccgattt tagccagaag ccaaagctgg aggcggtacc ggtcaaagcg gctgatgcgg
    12241 tgctgacgga cacgaccttt tctgcttacg caaccttacg ggtggattac caatgaaaac
    12301 agcacacatt tattcacccg gttatgcgct gttagcaggc ctgctgctga ccgccagcac
    12361 aggagcacag gcagtcgaca acaacctgca tttttacggc aacctgctga gtaaatcctg
    12421 cacgctggtg gtggatggtg ccaacctggc agaaatccat tttcccaccg tcagcaaccg
    12481 ggatttgatg gtatctgggc agtccgcgca tctgccggtt gtgtttaagc tgaaagactg
    12541 caaagggccg gcgggataca acgtcaaagt cacgctgagc ggcaccgaag acagcggtca
    12601 gccgggtttt ctggcagtcg atgacacctc caccgcgcag ggcgtgggga tagggatgga
    12661 aacgctcgac ggcaagcggg tggatattaa caacaccacc ggggcgacct ttgcgcttac
    12721 caacggcaac aatgatatta atttccgggc ctggatctag gcgaaaagcg gtcgtgatgt
    12781 aaccatcggt gatttcaccg ccagcctgac agcaactttt gagtatattt aaaatgaaaa
    12841 tccttcgtca tgttttatgt cttttattgc tgttaatgtt caatacatgc ggccaggcgg
    12901 cgacgaaagt tggtgagtta tatgtgccag ctggtgccag ttcacctgcg ac
//
LOCUS       DAJTMC010000116        12921 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.116, whole genome shotgun sequence.
ACCESSION   DAJTMC010000116 DAJTMC010000000
VERSION     DAJTMC010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12921
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.116"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(<1..880)
                     /gene="rarD"
                     /locus_tag="OO130_002935"
     CDS             complement(<1..880)
                     /gene="rarD"
                     /locus_tag="OO130_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HCR5366261.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMLLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFAFI
                     WVALAIFVMDAIYTQRR"
     gene            complement(932..1405)
                     /locus_tag="OO130_002936"
     CDS             complement(932..1405)
                     /locus_tag="OO130_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HCR5366262.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATATYMVG"
     gene            1570..2439
                     /gene="pldA"
                     /locus_tag="OO130_002937"
     CDS             1570..2439
                     /gene="pldA"
                     /locus_tag="OO130_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HCR5366263.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            2572..4401
                     /gene="recQ"
                     /locus_tag="OO130_002938"
     CDS             2572..4401
                     /gene="recQ"
                     /locus_tag="OO130_002938"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HCR5366264.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMCKLERFGKPFMALIRAHVDGDDEE"
     gene            4465..5085
                     /gene="rhtC"
                     /locus_tag="OO130_002939"
     CDS             4465..5085
                     /gene="rhtC"
                     /locus_tag="OO130_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HCR5366265.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQIELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTARWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(5147..5767)
                     /gene="rhtB"
                     /locus_tag="OO130_002940"
     CDS             complement(5147..5767)
                     /gene="rhtB"
                     /locus_tag="OO130_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HCR5366266.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            5878..6900
                     /gene="pldB"
                     /locus_tag="OO130_002941"
     CDS             5878..6900
                     /gene="pldB"
                     /locus_tag="OO130_002941"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HCR5366267.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            6908..7708
                     /gene="yigL"
                     /locus_tag="OO130_002942"
     CDS             6908..7708
                     /gene="yigL"
                     /locus_tag="OO130_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HCR5366268.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            7784..8683
                     /gene="bioP"
                     /locus_tag="OO130_002943"
     CDS             7784..8683
                     /gene="bioP"
                     /locus_tag="OO130_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HCR5366269.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMNKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTLITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(8571..9524)
                     /gene="metR"
                     /locus_tag="OO130_002944"
     CDS             complement(8571..9524)
                     /gene="metR"
                     /locus_tag="OO130_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HCR5366270.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            9760..12021
                     /gene="metE"
                     /locus_tag="OO130_002945"
     CDS             9760..12021
                     /gene="metE"
                     /locus_tag="OO130_002945"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HCR5366271.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLAVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPARHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHNVKPV
                     LLGPVTWLWLGKVKGEQFDRLSLLNDILPVYQQVLAELEKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDGVAEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(12306..12551)
                     /locus_tag="OO130_002946"
     CDS             complement(12306..12551)
                     /locus_tag="OO130_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR5366272.1"
                     /translation="MITETEINVLKVMAEKDINWNWMNLDRTLSIRQIPGFGNVVNIV
                     NKLANEGLVIIEDSGHKSMPHYHVTEKGYNFVKSENK"
     gene            complement(12541..>12921)
                     /locus_tag="OO130_002947"
     CDS             complement(12541..>12921)
                     /locus_tag="OO130_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5366273.1"
                     /translation="PFNPDLAGGPIENLTTDGVTINREGIAIVEKHIARFGHDPVNEV
                     MINRLKDIEKGKIPPEQVDLNFYTHECREYQRYCNLGWETGQPDGDAGYALWNHTHTA
                     TLEDYKLKGELNDLYHQDALDYDN"
BASE COUNT         3052 a         3262 c         3447 g         3160 t
ORIGIN      
        1 ttctacgctg cgtataaatc gcatccatca caaaaattgc cagcgccacc caaataaagg
       61 cgaaggtcac catcttatcg gcacccggtt tttcaccata aaacgtcact gccaacagga
      121 acatcagcgt cgggccaata tactggaaga agcctaacgt ggagagacgc aggcgtgtag
      181 cagctgcggt aaaacacaac agcggtacgg tggtgacaat accggcggcg atcagcagca
      241 aattcagcaa catcgggttt tgccccatat ggctggttga gctgtcggca atagcaaaca
      301 gataaatcgc cgccacgggc agcagccaca tggtttcgat taacatgccg gtttgcgctt
      361 caacggcaat tttcttgcgt accagtccgt agaaggcaaa actaaacgcc agccccagcg
      421 cgatgattgg cagcgaacca aaagtccaca actgaaccaa cacgccacat atcgccagaa
      481 tcaccgccag ccattgcatc cggcggaatc gctcgccgag gaaaatcatc cccagcacaa
      541 tgttcaccag cgggttaata aagtaaccaa ggctcgcttc cagcatatgg tgattgttca
      601 ccgcccagat aaacagtagc cagttgccac caatcagcac ggcagagact gccagcataa
      661 aaattttctg tggcgtctga atcagcgttt ttaaatagga ccactggcgg caaatgctca
      721 tcagcaccac cataaagaaa aacgaccaga tcacgcgatg cgtcaggatc tcatcggcgg
      781 gcacgtagta aatcagcttg aagtatgctg gcgcaatacc ccaaataaaa taagcggcaa
      841 gagcgagtaa tacgccctgc cgcgtttgtt ttgcatccat cgggaatact catttttaat
      901 tggtaacagc agtttacctg cttttatgtc ttcaacctac catataggtg gcggtggcgg
      961 tggcactggc aatataaagc tgttcttcat tgtgtaattc aacgcgggcg acggcgactt
     1021 tattgcctgc acgcaacagg ctactggtag cagtaaaacg ctcgcccctg cctgggcgca
     1081 gataatcaac gcgaagatca atggtcccca tccgcgatag ccgctggcgt agttcatctt
     1141 cactgatggt ttcgtggcgg gttaaggtac ttcccacgca caccagaccg gcggcgacat
     1201 ccagcgccga cgcaatgact ccgccgtgca aaatgctttg cgcccagtta cccaccatca
     1261 tcggctgatt tttaaaggcc agctgtgcga actctttttc gtaacgctcc agctccatcc
     1321 ctaacgcgcg gttaaatggc atgtgataaa caaacatctc acccactaat ttcagggctt
     1381 gttcagcggt cagtacggca gacatatcat ccttacactt cattggttaa tgaaatgttg
     1441 attttatgct tctttgttgt tggtttctac tttaggaagg gataactaac ggcaatggag
     1501 ttaagtatgt aaaatagccc gcagaaaaac attcacctta tcaataattc gttacggaga
     1561 acacgaccga tgcggactct gcagggctgg ttgttgccgg tgtttatgtt gcctatggca
     1621 gtatatgcac aagaggcaac ggtgaaagag gtgcatgacg cgccagcggt gcgtggcagt
     1681 attatcgcca atatgctgca ggagcatgac aatccgttca cgctctatcc ttatgacacc
     1741 aactacctca tctacaccca aaccagcgat ctgaacaaag aagcgattgc cagttacgac
     1801 tgggcggaaa atgcgcgtaa ggatgaagta aagtttcagt tgagcctggc atttccactg
     1861 tggcgtggga ttttaggccc gaactcggtg ttgggtgcgt cttatacgca aaaatcctgg
     1921 tggcaactgt ccaatagcga agagtcttca ccgtttcgtg aaaccaacta cgaaccgcaa
     1981 ttgttcctcg gttttgccac cgattaccgt tttgcaggtt ggacgctgcg cgatgtggag
     2041 atggggtata accacgactc taacgggcgt tccgacccga cctcccgcag ctggaaccgc
     2101 ctttatactc gcctgatggc agaaaacggt aactggctgg tagaagtgaa gccgtggtat
     2161 gtggtgggta atactgacga taacccggat atcaccaaat atatgggtta ctaccagctt
     2221 aaaatcggct atcacctcgg tgatgcggtg ttgagtgcga aaggacagta caactggaat
     2281 accggttacg gcggcgcgga gttaggctta agttacccga tcaccaaaca tgtgcgcctt
     2341 tatactcagg tttacagcgg ctatggcgaa tcgctcatcg actataactt caaccagacc
     2401 cgtgtcggtg taggagttat gctaaacgac ttgttttgat gaacggttga gtggttggca
     2461 aatctggaac ccagcatcca ggattaccct ctcagagact aaaagcattg cagtttctcg
     2521 cgcaggcgct gaaaatagcg cctgttttta tttcaggcaa tcggggtgaa tgtggcgcag
     2581 gcggaagtgt tgaatctgga gtccggagct aaacaggttt tacaagaaac ctttggctac
     2641 caacagtttc gccccggcca ggaagaaatt atcgacactg tgctttccgg tcgcgattgc
     2701 ctggtcgtca tgcccaccgg tggcggaaaa tccctttgct atcaaattcc tgccttattg
     2761 ctaaacggcc ttaccgtggt tgtttcaccg ctgatttcgt tgatgaaaga tcaggtggat
     2821 caactgcaag ccaacggcgt ggcggcggcg tgccttaact cgacgcaaac ccgcgagcag
     2881 caacttgaag tgatgacagg ctgccgcacc gggcaaattc gtctgcttta tatcgccccg
     2941 gaacgcctga tgctggataa ctttcttgag catctggcgc actggaatcc ggtgttatta
     3001 gccgttgatg aagcgcactg tatctcccaa tggggccacg atttccgccc ggaatatgcc
     3061 gcgcttggtc agttacgcca gcgtttcccg accctgccgt ttatggcgct gactgccaca
     3121 gccgacgaca ccacgcgcca ggatatcgtg cgcttgctgg ggctgaacga tccgctgatt
     3181 caaatcagca gttttgaccg tccgaatatt cgctacatgc tgatggagaa gttcaaaccg
     3241 ctcgatcagt tgatgcgcta cgtgcaggaa cagcgcggta agtcaggcat tatctactgc
     3301 aacagccgcg cgaaagtaga agacaccgct gcgcgcctgc aaagcaaggg aattagcgcg
     3361 gcggcctatc atgctgggct ggaaaataat gttcgcgccg acgtgcagga aaaatttcag
     3421 cgcgatgacc tgcaaattgt ggtggcgacg gtggcgttcg gcatgggcat caataaacca
     3481 aacgttcgct tcgtggtcca ctttgatatt ccgcgcaata tcgaatccta ttatcaggaa
     3541 accggtcgcg ccgggcgtga tggcctgccc gcggaagcga tgctgtttta cgatccggcc
     3601 gatatggcgt ggctgcgccg ttgcctggaa gagaagccgc aggggcagtt acaggatatc
     3661 gaacgtcata aactcaatgc gatgggcgct tttgccgaag cgcaaacttg ccgtcgtctg
     3721 gtattgctga actattttgg cgaagggcgg caagagccgt gcgggaactg cgatatctgc
     3781 ctcgatccgc cgaaacagta cgacggttca accgatgctc agattgccct ttccaccatt
     3841 ggtcgtgtga atcagcggtt tgggatgggt tatgtggtgg aagtgattcg tggtgctaat
     3901 aaccagcgta tccgcgacta tggtcatgac aaactgaaag tctatggcat gggccgtgat
     3961 aaaagccatg aacattgggt gagcgtgatc cgccagctga ttcacctcgg cctggtgacg
     4021 caaaatattg cccagcattc tgccctacaa ctgacagagg ccgcgcgccc ggtgctgcgc
     4081 ggcgaatcct ctttgcaact tgccgtgccg cgtatcgtgg cgctcaaacc gaaagcgatg
     4141 cagaaatcgt tcggcggcaa ctatgatcgc aaactgttcg ccaaattacg caaactgcgt
     4201 aaatcgattg ccgatgaaag taatgtcccg ccgtacgtgg tgtttaacga cgcaaccttg
     4261 attgagatgg ctgaacagat gccgatcacc gccagcgaaa tgctcagcgt taacggcgtt
     4321 gggatgtgca agctggaacg ctttggtaaa ccgtttatgg cgctgattcg tgcgcatgtc
     4381 gatggcgacg acgaagagta gtcagcagca taaaaaagtg ccaggatgaa gactccgtaa
     4441 acgtttcccc cgcgagtcaa atgtatgttg atgttatttc tcaccgtcgc catggtgcac
     4501 attgtggcgc ttatgagccc cggtcccgat ttcttttttg tctctcagac cgctgtcagt
     4561 cgttcccgta aagaagcgat gatgggcgtg ctgggtatta cctgcggcgt tatggtttgg
     4621 gcagggattg cgttgcttgg cctgcatttg attatcgaaa aaatggcctg gctgcatacg
     4681 ctgattatgg ttggcggtgg cctgtatctc tgctggatgg gttaccagat gctacgcggt
     4741 gcactgaaaa aagaggctgt atctgcgcct gcaccacaga ttgagctggc gaaaagcggg
     4801 cgcagtttcc tgaaaggttt actgaccaat ctcgctaatc cgaaagcgat tatctacttt
     4861 ggctcggtgt tctcattgtt tgtcggtgat aacgttggca ctaccgcgcg ctggggcatt
     4921 tttgcgctga tcattgtcga aacactggcg tggtttaccg tcgttgccag cctgtttgcc
     4981 ctgccgcaaa tgcgccgtgg ttatcaacgt ctggcgaagt ggattgatgg ttttgccggg
     5041 gcgttatttg ccggatttgg cattcatttg attatttcgc ggtgatgcca gacgcgtctt
     5101 cagagtaagt cggataaggc gtttacgccg catccgacat tatttttcac gcatgcctcg
     5161 ccgatgctaa cagcgctccc accagcataa acaacgagcc gaaaatctta ttcagcgcct
     5221 tcatctgctt tggtccttta atccatagag caatccgttg agcaagggtg gcgtaaccga
     5281 tcatcacaat aatatcgact acaatagtgg tgacgccgag cacgatatac tgcatcagtt
     5341 gcggctgctg tggcatgatg aattgcggaa atagcgccgc cagaaacaca atacttttgg
     5401 gattggtgag attcacaaaa actgcgcgct ggaacaaatg tcgacgcgat tgagtagagg
     5461 caagcgattt aaggtcaatt gcaccagcgg cgcgccactg ctggattccc agccaaatca
     5521 agtaagccgc gcctgcccac ttcaacactt caaacgcaat cactgagcgg gaaaatagcg
     5581 tccccaaacc cacgccaacc agcacaatat gaatcgccag tccggtctga agcccagcaa
     5641 tagacgccac cgcgccgcga taaccgtggt tgagcgaggt ggtcatagtg ttgattgcac
     5701 cagagcctgg cgacaggctt aaaatgatcg atgtcagcag gtaggcaaac caccattcta
     5761 aggtcatgat gaactcccgg tgtgtctatt tttgtgccac aatacgctac tgtcgcagcg
     5821 ttgtgtcagg cacgctaaaa aaaacgattt tacgtggttt aagaggcaga ttacccgatg
     5881 tttcagcagc aaaaagactg ggaaacaaga gaaaacgcgt ttgctgcttt taccatggga
     5941 ccgctgactg atttctggcg tcagcgtgat gaagcagagt ttactggtgt ggatgacatt
     6001 ccggtgcgct ttgtccgttt tcgcgcacag caccatgacc gggtggtagt catctgcccg
     6061 gggcgtattg agagctacgt aaaatatgcg gaactggcct atgacctgtt ccatttgggg
     6121 tttgatgtct taatcatcga ccatcgcggg cagggacgtt ccggtcgcct gttagccgat
     6181 ccgcatctcg ggcatgttaa tcgctttaat gattatgttg atgatctggc ggcattctgg
     6241 cagcaggagg tccagcccgg tccgtggcgt aaacgctata tactggcaca ttcgatgggc
     6301 ggtgcgatct ccacattatt tctgcaacgc catccaggtg tatgtgacgc cattgcgcta
     6361 actgcgccaa tgtttgggat cgtgattcgt atgccgtcat ttatggcacg gcagatcctc
     6421 aactgggccg aagcgcatcc acgtttccgt gatggctatg caataggcac cgggcgctgg
     6481 cgcgcgttgc cgtttgctat caacgtactg acccacagca gacagcgata tcgacgtaac
     6541 ttacgcttct atgctgatga cccaacgatt cgcgtcggtg ggccgaccta ccattgggta
     6601 cgcgaaagta ttctggctgg cgaacaggtg ttagccggtg cgggtgatga cgccacgcca
     6661 acgcttctct tgcaggctga agaggaacgc gtggtggata accgcatgca tgaccgtttt
     6721 tgtgaactcc gcaccgccgc gggccatcct gtcgaaggag gacggccgtt ggtaattaaa
     6781 ggtgcttacc atgagatcct ttttgaaaag gacgcaatgc gctcagtcgc gctccacgcc
     6841 atcgttgatt ttttcaacag gcataactca cccagcggaa accgctctac agaggtttaa
     6901 atttcttatg taccaggttg ttgcgtctga tttagatggc acgttacttt ctcccgacca
     6961 tacgttatcc ccttacgcca aagaaactct gaagctgctc accgcgcgcg gcatcaactt
     7021 tgtgtttgcg accggtcgtc accacgttga tgtggggcaa attcgcgata atctggagat
     7081 taagtcttac atgattacct ccaatggtgc gcgcgttcac gatctggatg gtaatctgat
     7141 ttttgctcat aacctggatc gcgacattgc cagcgatctg tttggcgtag tcaacgacaa
     7201 tccggacatc attactaacg tttatcgcga cgacgaatgg tttatgaatc gccatcgccc
     7261 ggaagagatg cgctttttta aagaagcggt gttcaaatat gcgctgtatg agcctggatt
     7321 actggagccg gaaggcgtca gcaaagtgtt cttcacctgc gattcccatg aacaactgct
     7381 gccgctggag caggcgatta acgctcgttg gggcgatcgc gtcaacgtca gtttctctac
     7441 cttaacctgt ctggaagtga tggcgggcgg cgtttcaaaa ggccatgcgc tggaagcggt
     7501 ggcgaagaaa ctgggctaca gcctgaagga ttgtattgcg tttggtgacg ggatgaacga
     7561 cgccgaaatg ctgtcgatgg cggggaaagg ctgcattatg ggcagtgcgc accagcgtct
     7621 gaaagacctt catcccgagc tggaagtgat tggtactaat gccgacgacg cggtgccgca
     7681 ttatctgcgt aaactctatt tatcgtaatc gttctttatt tggtcagttg tcaacctgat
     7741 acttcgctac aatggatact cgttaatcaa agagttttcc attgtggcgc tacttatcat
     7801 caccacgatt ctgtgggcct tctcctttag cttttatggc gagtaccttg cggggcacgt
     7861 cgatagctat tttgcggtgc tggtgcgcgt tggcctggcg gcactcgttt ttctgccgtt
     7921 tctgcgtacc cgtggcaata gcctgaaaac agtcggcctg tatatgctgg tgggcgcgat
     7981 gcagctcggc gtgatgtata tgctgagttt ccgcgcttat ctctatctga cggtttccga
     8041 actgctgctg ttcaccgtac tgacgccgct ctacatcacg ctgatttatg acatcatgaa
     8101 taagcgccgt ctgcgctggg gatatgcttt tagtgccttg ttggcggtga ttggtgccgg
     8161 gattattcgc tatgatcagg tcaccgacca tttctggact ggcttgttgc tggtgcaact
     8221 ctccaatatc acttttgcca tcggcatggt gggttacaaa cgcctgatgg aaactcgccc
     8281 gatgccgcag cataacgcct ttgcgtggtt ctatcttggc gcgttcctgg tggcggtaat
     8341 tgcatggttc ttgctgggaa atgcgcagaa aatgccgcaa accacgttgc aatggggcat
     8401 tctggtgttt cttggcgtgg tggcttccgg gattggctac tttatgtgga actacggcgc
     8461 gacgcaggtg gacgccggaa cgctgggaat tatgaataat atgcacgttc cggcagggct
     8521 gctggtaaac ctggctatct ggcaccaaca gccgcactgg ccaacgttga ttacaggcgc
     8581 gctggtgatc ctggcctcac tgtgggtgca tcgtaagtgg gtcgctccgc gctcttcaca
     8641 aacggcagat gatcgcaggc gtgattgcgc gctgagcgaa taaacgcttc cgtaactggc
     8701 tgacgctgct cgccatcgcg cacggcggcg tacagtcggc tccacaagcc ttcgcccagg
     8761 gttttggtca ccaccagacc ctggcgctca aaactctcta ctacccaatg cggtagcgcg
     8821 gcaataccca tccgcgcggc aaccatctga atcaacaata aggtgttatc gacgcttttc
     8881 agtgacggac tgacgcctgc cggctgaaga aaatgccgcc agacatccag tcgactacgc
     8941 tgcaccggat aaattaatag cgtctcgctg gcgagatctt ccggtgtaat acgcgttttt
     9001 gccgccagtg gatggtcagg tgctaacacc agacgcactt catagtcgaa catcggcgaa
     9061 taatgcagac cactgcgcgg cagaatatcg gacgtcatta ccagatccag ctctccctgt
     9121 tgcaaggcgg gctgcgggtc aaatgtcacg cccgatttaa agtccatctc gacctgcggc
     9181 cagttcttat ggaaattttc taacgcgggc gtcagccact gtatacagct atggcactca
     9241 atggcaatgc gcagacgcgt ctgctgcggt tcattgcagg cttgcagtgc ctggctaatt
     9301 tgtggcagca cctggtttgc cagttgcaac aggatttctc cctgcggtgt aaagcgaagc
     9361 ggctggcttt tacgcacaaa tagccggaag ccaaggcgtt gttccagatc gctaaactgg
     9421 tgagacaggg cggattgcgt ttgatgcaac gtcgccgcag cggctgcgag tgagccgcag
     9481 ttccgcaacg cttgtagcgt tttcagatgt tttacttcga tcatgaaagt ccttcacttc
     9541 ggcatgaata atttgcgctt gaggaatata cagtaaccgc caattatgga tgtgtaaaca
     9601 tctggacggc taaaatcctt cgtcttttaa atttatggtg cgttggctgc gtttctccac
     9661 cccggtcact tacttcagta agctcccggg gatgaataaa cttgccgcct tccctaaatt
     9721 caaaatccat aggatttaca tataattaga ggaagaaaaa tgacaatatt gaatcacacc
     9781 ctcggtttcc ctcgcgttgg cctgcgtcgc gagctgaaaa aagcgcaaga gagttattgg
     9841 gcagggaact ccacgcgtga agaactgctg gcggtagggc gtgaattgcg tgctcgtcac
     9901 tgggatcaac aaaagcaagc gggtatcgac ctgctgccgg tgggcgattt tgcctggtac
     9961 gatcatgtac tgaccaccag tctgctgctg ggtaacgttc cggcgcgtca tcagaacaaa
    10021 gatggttcgg tagatattga caccctgttc cgtattggtc gtggacgtgc gccgactggc
    10081 gaacctgcgg cggcagcgga aatgaccaaa tggtttaaca ccaactatca ctacatggtg
    10141 ccggaattcg ttaaaggcca acagttcaaa ctgacctgga cgcagctgct ggaggaagtg
    10201 gacgaggcgc tggcgctggg ccacaacgtt aagcctgtgc tgctggggcc ggttacctgg
    10261 ctgtggctgg ggaaagtgaa aggtgaacag tttgaccgcc tgagcctgct gaacgacatt
    10321 ctgccggttt atcagcaagt gctggcagaa ctggagaaac gcggcatcga gtgggtacag
    10381 attgatgaac ccgcgctggt actggaacta ccacaggcgt ggctggacgc atacaaaccc
    10441 gcttacgacg cgctccaggg acaggtgaaa ctgctgctga ctacctattt tgaaggcgtt
    10501 acgccaaatc tcgacacgat tactgcgctg cctgttcagg gactgcatgt ggaccttgta
    10561 catggtaaag atggcgttgc tgaactgcac aagcgcctgc cttctgactg gctgctgtct
    10621 gcgggactga tcaatggtcg taacgtctgg cgcgccgatc ttactgagaa atatgcgcaa
    10681 attaaggaca ttgtcggcaa acgtgatttg tgggtggcat cttcctgctc gttgctgcac
    10741 agccccatcg acctgagcgt ggaaacgcgt cttgatgcag aagtgaaaag ctggtttgcc
    10801 ttcgccttac aaaaatgcca tgaactggca ctgctgcgcg atgcgctgaa cagtggtgac
    10861 acggcagctc tggcagagtg gagcgcccca attcaggcgc gtcgtcactc tactcgtgta
    10921 cataatccgg cggtagaaaa gcgtctggcg gcgatcaccg cccaggacag ccagcgtgcg
    10981 aatgtctatg aagtgcgtgc tgaagcccag cgtgcgcgtt ttaaactgcc agcgtggccg
    11041 accaccacga ttggttcctt cccgcaaacc acggaaattc gtaccctgcg tctggatttc
    11101 aaaaagggca atctcgacgc caacaactac cgcacgggca ttgcggaaca tatcaagcag
    11161 gccattgttg agcaggaacg tttgggactg gatgtgctgg tacatggcga ggccgagcgt
    11221 aatgacatgg tggaatactt tggcgagcac ctcgacggat ttgtctttac gcaaaacggt
    11281 tgggtacaga gctacggttc ccgctgcgtg aagccaccga ttgttattgg tgacgttagt
    11341 cgcccggcac cgattaccgt ggagtgggcg aagtatgcgc aatctctgac tgataaaccg
    11401 gtgaaaggga tgctgactgg cccggtgacc attctctgct ggtcgttccc gcgtgaagat
    11461 gtcagccgtg aaaccatcgc caaacaaatt gcgctggcgc tgcgtgatga agtggcggac
    11521 ctggaagccg caggaattgg catcatccag attgatgaac cggcgctgcg cgaaggttta
    11581 ccgctgcgtc gtagcgactg ggatgcatat ctccagtggg gcgtggaggc cttccgtatc
    11641 aacgccgccg tggcgaaaga tgacacacaa atccacactc acatgtgtta ttgcgagttc
    11701 aacgacatca tggattccat tgcggcactg gatgcagacg tcatcaccat cgaaacctcg
    11761 cgttccgaca tggagttgct ggagtcgttt gaagagtttg attatccgaa tgaaatcggt
    11821 ccaggcgtct atgacattca ctcgccaaac gtaccgagcg tagaatggat tgaagccttg
    11881 ctgaagaaag cggcaaaacg cattccggca gagcgcctgt gggtcaaccc ggactgtggc
    11941 ctgaaaacgc gcggctggcc agaaacccgc gcggcactgg cgaacatggt gcaggcggcg
    12001 cagaatttgc gtcggggatg atgaatatta acgagtcact gtaaattgca gtacgatgaa
    12061 agaggcaaaa ctgcctgata ccaggcctac ataatttatg caatttattg aatttttgag
    12121 ttttgttggc cggacaaggc gttcacgccg catccggcat gaacaacgcg cactttgcca
    12181 ataatctgat ggcttccatc accggaagcc attttgctat ctgcgcccgt tcagatacag
    12241 aaataaccga atcattttta cgcaaaatat aatcctgagc gatttgttca gtaatcaacg
    12301 ctacgttatt tattctcact tttcacgaaa ttgtatccct tttcagtaac gtggtagtga
    12361 ggcatagact tatgtccgct atcctcaata ataaccaaac cctcgttagc taatttgtta
    12421 acaatgttaa caacattacc gaatcctgga atttgcctta tggatagtgt gcgatcgaga
    12481 ttcatccagt tccagtttat atctttctca gccataactt tcagtacgtt aatttcagtt
    12541 tcagttatca tagtcaagtg catcctggtg gtataagtca ttaagttctc ccttaagttt
    12601 ataatcttct agagtagcag tatgcgtatg attccagagt gcatagcctg cgtcaccatc
    12661 aggttgtcct gtttcccaac caagattaca gtaacgctga tattcccgac actcatgagt
    12721 atagaagtta agatctactt gttctggggg gatttttcct ttctcaatgt ctttcagtct
    12781 attgatcatc acttcattta cagggtcatg accaaatctg gctatgtgtt tttcaacaat
    12841 agcgatacct tccctgttga ttgttacgcc atctgtcgtc agattttcta ttggaccacc
    12901 ggcaaggtcg ggattaaatg g
//
LOCUS       DAJTMC010000117        12846 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.117, whole genome shotgun sequence.
ACCESSION   DAJTMC010000117 DAJTMC010000000
VERSION     DAJTMC010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12846)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12846)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12846
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.117"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(94..1185)
                     /gene="ychF"
                     /locus_tag="OO130_002948"
     CDS             complement(94..1185)
                     /gene="ychF"
                     /locus_tag="OO130_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HCR5366274.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(1302..1886)
                     /gene="pth"
                     /locus_tag="OO130_002949"
     CDS             complement(1302..1886)
                     /gene="pth"
                     /locus_tag="OO130_002949"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCR5366275.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            2164..2442
                     /gene="ychH"
                     /locus_tag="OO130_002950"
     CDS             2164..2442
                     /gene="ychH"
                     /locus_tag="OO130_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HCR5366276.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(2497..4176)
                     /gene="dauA"
                     /locus_tag="OO130_002951"
     CDS             complement(2497..4176)
                     /gene="dauA"
                     /locus_tag="OO130_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HCR5366277.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVIIYPVSQQFGL
                     AGLLVAALLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPISGRLAFFPNRRAAMADL"
     gene            complement(4301..5248)
                     /gene="prs"
                     /locus_tag="OO130_002952"
     CDS             complement(4301..5248)
                     /gene="prs"
                     /locus_tag="OO130_002952"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HCR5366278.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(5399..6250)
                     /gene="ispE"
                     /locus_tag="OO130_002953"
     CDS             complement(5399..6250)
                     /gene="ispE"
                     /locus_tag="OO130_002953"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HCR5366279.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            complement(6250..6873)
                     /gene="lolB"
                     /locus_tag="OO130_002954"
     CDS             complement(6250..6873)
                     /gene="lolB"
                     /locus_tag="OO130_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HCR5366280.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTHPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            7087..8343
                     /gene="hemA"
                     /locus_tag="OO130_002955"
     CDS             7087..8343
                     /gene="hemA"
                     /locus_tag="OO130_002955"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HCR5366281.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            8385..9467
                     /gene="prfA"
                     /locus_tag="OO130_002956"
     CDS             8385..9467
                     /gene="prfA"
                     /locus_tag="OO130_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HCR5366282.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELRKAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            9467..10300
                     /gene="prmC"
                     /locus_tag="OO130_002957"
     CDS             9467..10300
                     /gene="prmC"
                     /locus_tag="OO130_002957"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCR5366283.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            10297..10689
                     /locus_tag="OO130_002958"
     CDS             10297..10689
                     /locus_tag="OO130_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HCR5366284.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            10693..11502
                     /locus_tag="OO130_002959"
     CDS             10693..11502
                     /locus_tag="OO130_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
                     /protein_id="HCR5366285.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            11538..12392
                     /gene="kdsA"
                     /locus_tag="OO130_002960"
     CDS             11538..12392
                     /gene="kdsA"
                     /locus_tag="OO130_002960"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HCR5366286.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(12541..12648)
                     /locus_tag="OO130_002961"
     CDS             complement(12541..12648)
                     /locus_tag="OO130_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCR5366287.1"
                     /translation="MTLAQFAMIFWHDLAAPILAGIITAAIVGWWRNRK"
BASE COUNT         3190 a         3272 c         3333 g         3051 t
ORIGIN      
        1 cggtgaaatg aaaaatccac gcaattgcgt ggattttata tacttttgcg ctcttcatga
       61 gatttagcga aacctcatga gacaataaat taattagacg ttgaaaagga agttcatcac
      121 atcgccatct ttcacgatgt aatctttacc ttctgcacgc attttgcctg cttctttcgc
      181 gccttgttca cctttgtaag tgatgaaatc ttcaaacgag atggtttgtg cacggataaa
      241 gcctttttca aaatcagtat ggattttgcc cgctgcctgc ggcgcggttg ctccaaccgg
      301 aatggtccat gcacgcactt ctttcacccc agcggtgaag taagtttgca ggttcagcag
      361 cttataaccg gcacggatca cacggttcag gcccggctct tccagcccaa gctcctgcat
      421 aaactcgtca cgttcttcgt cgtccagttc ggcaatgtct gcttcaacag cagcacaaac
      481 cggaaccaca acagaacctt ctttcgccgc gatttcacgc acctggtcaa gatatgggtt
      541 gttttcaaaa ccgtcttcgt tgacgttggc gatgtacatt gttggtttca gcgtcaggaa
      601 gctcaggtaa cgaatagccg ctttctcttc agcgcttaaa tccagcgcgc gcagcatacc
      661 tgcattttcc aactggggca ggcatttttc caggaccgcc agctcagctt tcgcgtcttt
      721 atcgccacct ttggctttct tctgtacgcg atgaatcgca cgttcgcagg tgtcgaggtc
      781 tgccagcgcc agttcggtgt tgataacttc aatatcgtca gccgggttaa ctttgccgga
      841 aacgtgaatg atgttgtcat tttcaaagca gcgaacaacg tgaccgatcg cttcggtttc
      901 acggatgttg gtcaggaact ggttacccag accttcgcct ttcgatgcgc cttttaccag
      961 accggcgata tcgacaaatt ccatggtcgt gggaagcgta cgctgcggtt ttacgatttc
     1021 agccagttga tccaggcgag gatcaggcat tggtacgacg cctgtgttcg gctcaatggt
     1081 gcagaatgga aagttggccg cttcaatacc ggctttggtc agcgcgttga acagggtaga
     1141 tttcccgacg ttgggcaaac cgacgatacc gcatttgaat cccatgattt aaatcacctt
     1201 aatatcttaa taatcaacct gttattgcta acagattgca gaaatggaaa taactttgcc
     1261 tattatacac ggcactcggc aaaaatgccg cagacaacga cttattgcgc tttaaaggcg
     1321 tgcaatcggt tcgttgcttt ggtcaagcca tctgtaaacc acatttcagt acaacgcgcc
     1381 gcttcgtcaa tggcttcatc aattaacttc tgttcactaa caggcggttt gcctaacaca
     1441 aaaccgacaa ctttattttt atcgcccgga tgaccgattc cgatgcgtaa acggtgaaag
     1501 ttagggttat tacccaattt actgatgatg tctttcagtc cattgtgacc accatggcca
     1561 ccgcccaatt taaatttggc gacgccagga ggcagatcca gttcgtcgtg ggccaccaga
     1621 atttcgtccg gattaatgcg gaaaaaactg gccatcgccg caacggcttt gccgctgaga
     1681 ttcataaatg tagtcgggac taacaggcgg acatcttcgc ctccaagagt gactcgcgaa
     1741 gtataaccaa agaatttagc ctcttcgcgc agcggagcgc gcaaacgctc tgccagtaag
     1801 tcaacgaacc aggcaccagc attatgtcgc gttgcggcgt attcagcacc ggggttcgcc
     1861 aggccgacaa tcaatttaat cgtcacgttt ttttgtcctg agtgtgtaca taactggcgc
     1921 gtagtttact ggttgcgacc ccgcttgaca aaaaactgcg tatcaaatgc agataacgta
     1981 ataattgcct gagtgggcta ttagaaagtc aaggtgttca ggcgtttatt tgtaaagttt
     2041 tgttgaaata agggttgtaa ttgtgatcac gcccgcacat aacccacagg gtgttgtcta
     2101 tactttacac ataaggaaga ggggtattcc ctgttacaac ccagaaagtt ccggaggtga
     2161 catatgaaac gcaaaaacgc ttcgttactc ggtaacgtgc tcatggggtt gggtctggtg
     2221 gtaatggtgg tcggcgtggg gtattcaatc ctcaaccagt taccacagtt taatatgccc
     2281 cagtatttcg cacatggtgc agtgctaagt attttcgtcg gtgccattct ctggctggcg
     2341 ggtgcccgtg ttggcgggca tgaacaggtg tgcgaccgtt actggtgggt tcgccactat
     2401 gacaaacgtt gccgccgtag cgataatcgc cgtcatagct aacaaataat gcagtttgct
     2461 gaccagtcag tttgcgctga ctggtcaatc tcgtacttat aaatccgcca tcgccgcgcg
     2521 acgattcggg aagaacgcga ggcgtcccga gattggttga atgcctgcgc gagccatagt
     2581 gcgcagtggc tggaattcca cgttgcacac gcgcagttca catccttcgg gcagacgctt
     2641 cacaaaacgc tggaacgcat caagaccacc agcatcaagt accggaacgg catcccactt
     2701 cagaatcaca atccgtttgc cttcaagacg tgactccagg tccgtgaaca agccttcagc
     2761 agcagcaaaa aacagcgggc caataacgcg cagtaccaga acatcgtctg gaacatctac
     2821 gactaccggt gccaggcgag tcatacgtgc gatacgacgc ataaacagca gcgatgccag
     2881 cacgatcccc acgctgatgg caataaccat atcaaacagc acggtcagcg acatgcacag
     2941 cagcatgaca atgatgtcat ctttcggtgc atgacgcagc aagtcgacca ctttatgcgc
     3001 ttcactcatg ttccacgcca ccatcaacag cagggctgcc atagcggaaa gcggcagcca
     3061 ggagagcagc ggtgccagta ccagcagggc aagaataacc agaatagagt ggatcaccgc
     3121 tgagataggg gaagttgccc cggcacggac gttagcggca gaacgcgcga tggcagctgt
     3181 agcggtaata ccaccaaaga atggagcgat aatattcccc agtccctgtc caaccagttc
     3241 gctgttcgcc ttgtgtttcg tcccggtcat accatccagt accacggcgc agagcagaga
     3301 ttcgattgcg ccgagcattg ccattgagaa tgccgcaggc agcagtgtgc gaatagaatc
     3361 ccaggttagc gtgaattctg aattaggcag atcccacggc agcaccagtt gcggcagcag
     3421 ttgcggaata ccgttaccct gagaaccatc ggccaggacg tagtggaatt gcgaaccgat
     3481 ggtagcaaca tgtccgccga gcaggttaac aatccccatc accgcgcaac cagccagcaa
     3541 ggccggaagg tgaccgggta aacgaatgcc cagacgcggc caaaaaacaa gaatacctag
     3601 cgtcacaatg ccaatggcag catcacccac attaatggtc ggcagcgcca taaataatgc
     3661 gccgactttt tgtagataat gttccgggac atgggccatt tgcagaccga gaaaatcttt
     3721 aatctgcatg gtaccgatgg tgatcccgat acccgaggtg aaacctaagg tgacggaaac
     3781 cggaatatac tcaatcaggc gaccaaagcg tgccagaccc atcagaatca aaaagatccc
     3841 cgacagcaag gccgcaacca gcagtcctgc cagtccaaac tgttgcgaca cgggatagat
     3901 aattaccaca aatgccgcag tcggaccgga aacgctaaag cgtgacccac ccgtcagagc
     3961 aatgacaatc cccgcaacag ctgcggtata taaaccgtac tggggtgcca caccactacc
     4021 aatagccaac gccatcgcca gcgggatagc aataatcccg acggttatcc cggcaatcag
     4081 gtcacgggta aaccgtgcgg cagtatattt ttctttccag caagcgtcga tcagagcgcg
     4141 gaaaggcatc acatgtgagg aaaatatttt gttcacaata atgtttcatc cgtgagcgca
     4201 tcatctgtca actaaatggc aggtgaagga ggcataggtc atacaaatgg atattacaga
     4261 caaaaaaacc cgccgcagcg ggtctttgag ccgggttcga ttagtgttcg aacatggcag
     4321 agatcgattc ttcgttgctg atacgacgaa tcgcttcggc cagcatacct gacagggtca
     4381 gagtacgcac gttcggcagt gatttgattt catcgctcag cggaatggta tcgcagacaa
     4441 cgacttcatc aattacagag ttacgcaggt tgttcgccgc gttgccagag aagatcgggt
     4501 gagtcgcgta cgcaaataca cgtttagcac cacgttcttt cagagcttca gcagctttac
     4561 acagcgtacc gccagtgtcg atcatatcat cgaccagtac gcagtcacga cctgcaacgt
     4621 caccgatgat atgcatcacc tgggaaacgt tcgcacgcgg acgacgtttg tcgatgattg
     4681 ccatatcggt atcgttcagc agcttagcga tagcgcgggc acgcacaacg ccgccgatgt
     4741 ccggagaaac cacaattggg ttatccagat tcagctgcag catgtcttcc agcaggatcg
     4801 ggctaccaaa tacgttatca accggaacgt cgaagaaacc ctgaatctgt tcagcgtgca
     4861 gatccactgt cagcacacgg tcaacaccga cgctggagag gaagtctgca accactttcg
     4921 cagtgattgg tacacgagcg gaacggacgc gacggtcctg gcgcgcatag ccaaagtagg
     4981 ggataacagc ggtgatacga cctgcggaag cacgacgcag ggcatcaacc ataacgacta
     5041 attccatcag gttgtcgtta gtaggggcac aagtggactg gatgatgaaa atatcaccac
     5101 cgcgtacatt ttcattaatt tgtacgctga cttcgccatc gctaaagcga cctacagcgg
     5161 cgtcgccgag tgaagtgtac aggcggttgg caatacgttg tgctagttcc ggggtggcgt
     5221 taccagcaaa aagcttcata tcaggcacga gaagaacctc aggcatgcgt ccattggtgg
     5281 aaagaatctg ccgaaaactg tgcgggccag gcatgatcct ttccaggcag tgtattaaag
     5341 agcgcgatgc aacgtctgga acaaggtgac gttgtcaccg aaactcagct tgcccggctt
     5401 aaagcatggc tctgtgcaat ggggaaagat taacgccttt cgccacaaag ccattgagcc
     5461 attccggggc ttgctctagc acctggcggg cttcagactc tgtatcaaat tcagcaaaga
     5521 cacaggcccc tgtcccagtc aggcgcgacg gggcgtattc taacagccag gaaagcaccg
     5581 catcaacctc gcgaaaacgt tttcttgcga taacctcgca atcattgctg aattcacatt
     5641 ttagcaacgt ttctattgac ctttttggcg tattgcgcgg gagttcagga tctttaaaaa
     5701 tcaccggagt cggaatactt acaccagggt gcgccaccag ataccacttc tctggcggat
     5761 ccaccggcgt tagtatttca ccaacgcctt cggcaaacgc ggcatgcccc cgaacaaaga
     5821 caggaacatc tgcgcccagc gtcagcccca tttccgccag ctcatccatg cttagcccgc
     5881 attgccagag atgatttaat gccaccagga ccgtcgcggc attggatgaa ccaccgccga
     5941 gaccgccgcc catcggcaaa cgcttgtcaa tgctgatatt cgcaccgctt cccgtcggaa
     6001 gacgcccgct gtctgccgca gttttcatca acaatcgcgc tgcgcgaacg atcaggttat
     6061 cttcatgttc cacgccttca acgggcgtta acagacgaat atccccatcg tcacgaagct
     6121 caatgctgat ggtgtcgccg taatcaagaa actgaaacag cgtttgcagc gtgtggtaac
     6181 catccgcacg ctgaccggta atgtataaaa acagattaag ttttgccgga gagggccact
     6241 gtgtccgcat tatttcacta tccagttatc catttttaac ttgatgcgtt gaccaccgtc
     6301 ggtgagttcc atattggctg gcatcgcagg atgcgttttg gtgtcataac caccataaac
     6361 aaccttccag tttttgccat tctggctgta ggtaatttcg ctcaggcggt actggtcgtc
     6421 cagtttgtag tcggttgcat cacccggtaa acctaaaatc cactggcgca agctgttgag
     6481 cggaattggc attccggtca atttgccaat catctcttcg gcgtcatcgg cggtataacg
     6541 ctgaccttta ttgtcgacta actgcacgtt acccggttga gcattcagct ccagttccgt
     6601 gctgcccaat gggttagtga gcagcagacg gtagcgatcc tggccggttt gctgccagaa
     6661 aaagcgggcg tacacttttt gttggtcaga aatataagcg aacgcgccgc gagtctgata
     6721 ctgattaaga ttgcgcacgt cttgctgatg ctgacgccat tgtggcgaat ccgggctttt
     6781 gccaggacct ttgggcgtgg taacggaaca ggcagtgagc acaagagcag ccagcggtag
     6841 caggcggata agacgaaaat cgggcagggg catagtgatg acaagtcctt gagatacgtt
     6901 gcagttataa cccttaatgc tagcgttacc gtccgctatc gtctatgttc aagttgtctt
     6961 aattgccaga atctaacggc tttcggcaat tactccaaaa gggggcgctc tcttttattg
     7021 atcttacgca tcctgtatga tgcaagcaga ctaactctat caacgttggt attatttccc
     7081 gcagacatga cccttttagc actcggtatc aaccataaaa cggcacctgt atcgctgcga
     7141 gaacgtgtat cgttttcgcc ggataagctc gatcaggcgc ttgacagcct gcttgcgcag
     7201 ccgatggtgc agggcggcgt ggtgctgtcg acgtgcaacc gcacggaact ttatcttagc
     7261 gttgaagagc aggataacct gcaagaggcg ttaatccgct ggctttgcga ttatcacaat
     7321 cttaatgaag aagatctgcg taaaagcctc tactggcatc aggataacga cgcggttagc
     7381 catttaatgc gtgttgccag cggcctggat tcactggttc ttggggagcc gcagatcctc
     7441 ggtcaggtta aaaaagcgtt tgccgattcg caaaaaggtc atatgaaggc cagcgaactg
     7501 gaacgcatgt tccagaaatc tttctctgta gcgaaacgcg ttcgcactga aacagatatc
     7561 ggtgccagtg ctgtgtctgt cgcttttgcg gcttgtacgc tggcgcggca gatctttgaa
     7621 tcgctctcta cggtcacagt gttgctggta ggcgcgggcg aaaccatcga gctggtagcg
     7681 cgtcatctgc gcgaacataa agtacagaag atgattatcg ccaaccgcac tcgcgaacgt
     7741 gcccaaattc tggcagatga agttggcgcg gaagtgattg ccctgagtga gatcgacgaa
     7801 cgtctgcgcg aagccgatat catcatcagt tccaccgcca gcccgttacc gattatcggg
     7861 aaaggcatgg tggagcgcgc attaaaaagt cgtcgtaacc aaccaatgct gttggtggat
     7921 attgccgttc cgcgcgatgt tgagccggaa gttggcaaac tggcgaatgc ttatctttat
     7981 agtgtggacg atctgcaaag catcatttcg cacaacctgg cgcagcgtaa agccgcagcg
     8041 gttgaggcgg aaactattgt cgctcaggaa accagcgaat ttatggcgtg gctgcgagca
     8101 caaagcgcca gcgaaaccat tcgcgagtat cgcagccagg cagagcacgt tcgcgatgag
     8161 ttaaccgcca aagcgttagc ggcccttgag cagggcggcg acgcgcaagc cattatgcag
     8221 gatctggcat ggaaactgac taaccgcttg atccatgcgc caacgaaatc acttcaacag
     8281 gccgcccgtg acggggataa cgaacgcctg aatattctgc gcgacagcct cgggctggag
     8341 tagcagtaca tcattttctt tttttacagg gtgcatttac gcctatgaag ccttctatcg
     8401 ttgccaaact ggaagccctg catgaacgcc atgaagaagt tcaggcgttg ctgggtgacg
     8461 cgcaaactat cgccgaccag gaacgttttc gcgcattatc acgcgaatat gcgcagttaa
     8521 gtgatgtttc gcgctgtttt accgactggc aacaggttca ggaagatatc gaaaccgcac
     8581 agatgatgct cgatgatcct gaaatgcgtg agatggcgca ggatgaactg cgcaaagcta
     8641 aagaaaaaag cgagcaactg gaacagcaat tacaggttct gttactgcca aaagatcctg
     8701 atgacgaacg taacgccttc ctcgaagtcc gagccggaac cggcggcgac gaagcggcgc
     8761 tgttcgcagg cgatctgttc cgtatgtaca gccgttacgc cgaagcccgc cgctggcggg
     8821 tagaaatcat gagcgccagc gagggtgaac atggtggtta taaagagatc atcgccaaaa
     8881 ttagcggtga tggtgtgtat ggtcgtctga aatttgaatc tggcggtcat cgcgtgcagc
     8941 gtgttcctgc tacggaatcg cagggtcgta ttcatacttc tgcttgtacc gttgcggtaa
     9001 tgccagaact gcctgacgca gaactgccgg acatcaaccc agcagattta cgcattgata
     9061 ctttccgctc gtcaggggcg ggtggtcagc acgttaacac caccgattcg gcaattcgta
     9121 ttactcactt gccgaccggg attgttgttg aatgtcagga tgaacgttca caacataaaa
     9181 acaaagcgaa ggcgctttca gtgctcggtg ctcgcatcca cgctgctgaa atggcaaaac
     9241 gccaacaggc cgaagcgtct acccgtcgta acctgctggg gagtggcgat cgcagcgacc
     9301 gtaaccgtac ttacaatttc ccgcaggggc gcgttaccga tcaccgcatc aacctgacgc
     9361 tctaccgcct ggatgaagtg atggaaggta agctggatat gctgattgaa ccgattatcc
     9421 aggaacatca ggccgaccaa ctggcggcgt tgtccgagca ggaataatgg aatatcaaca
     9481 ctggttacgt gaagcaataa gccaacttca ggcgagcgaa agcccgcggc gtgatgctga
     9541 aatcctgctg gagcatgtta ccggcagagg gcgtactttt attctcgcct ttggtgaaac
     9601 gcagctgact gacgaacaat gtcagcaact tgatgcgcta ctgacacgtc gtcgcgatgg
     9661 tgaacccatt gctcatttaa ccggggtgcg agaattctgg tcgttgccgt tatttgtttc
     9721 gccagcgacc ttaattccgc gcccggatac ggagtgtctg gtggagcagg cactggcgcg
     9781 gttgcctgaa caaccttgcc gtattcttga tctcgggacg ggtaccgggg cgattgcgct
     9841 tgcgctggct agcgagcgcc cggactgcga aattatcgct gtagatcgta tgcctgatgc
     9901 tgtctccctg gcacaacgta atgcccagca tctggcgatc aaaaatatcc acattctgca
     9961 aagcgactgg tttagcgcgc tagccgggca gcagtttgcg atgattgtca gcaatccgcc
    10021 gtatattgac gagcaggacc cacatcttca acaaggcgat gtccgctttg agccgctcac
    10081 tgcgctggtt gcggcagaca gtggaatggc agacatcgtg catatcatcg aacagtcgcg
    10141 taacgcgctg gtatccggcg gctttctgct tctggaacat ggctggcagc agggcgaagc
    10201 ggtgcgacaa gcatttatcc tcgcggggta tcatgacgtc gaaacctgcc gtgactatgg
    10261 tgataacgag cgcgtaacgc tcggccgcta ttatcaatga caagtttttc tacactgctt
    10321 agtgttcatc ttattagtat cgcgctttct gttgggctat taaccttacg tttctggcta
    10381 cgttatcaga agcatccaca ggcatttgct cgctggacgc gcattgtgcc gccggttgtc
    10441 gatacggtgt tattgttaag cggcattgcg ttgatggcta aagcgcacat cctgccattt
    10501 tccgggcagg cacagtggct gactgaaaag ctgtttggag ttatcattta tatcgttttg
    10561 ggttttattg cactcgatta tcgtcgtatg cacagtcagc aggcgcgcat tattgccttc
    10621 ccgctggcgt tggtggtgct gtacatcatc attaaactcg ccaccacaaa agtaccgtta
    10681 ctggggtaag tcatgagatc gttagctgat ttcgaattta ataaagcgcc attgtgcgaa
    10741 ggcatgatcc tggcttgcga agcaatccgc cgcgattttc cctcgcaaga tgtttacgac
    10801 gaactggagc gtctcgttag tctggcgaag gaagaaatca gccagcttct gcctttagaa
    10861 gagcagctgg aaaaactgat cgcgctgttt tacggcgact ggggatttaa agcctcacgc
    10921 ggcgtttatc gtctttccga tgcattatgg ctggaccagg tgttaaagaa tcgacagggc
    10981 agtgcggtat cattaggtgc ggttttatta tgggtcgcga atcgtctcga tttgccgctg
    11041 ctgccggtga ttttccctac gcagctgata ttgcgcattg aatgtccgga tggcgaaatt
    11101 tggctgatta atccttttaa cggtgaatcg ttaagcgaac atatgctgga cgtatggtta
    11161 aagggaaata tcagcccgtc ggcggaactg ttttatgaag accttgatga agctgataac
    11221 attgaggtaa tccgcaaatt gctggataca ctcaaagcct cgttgatgga agaaaatcag
    11281 atggagctgg cgttacgcac cagcgaagct ttattacaat tcaaccctga agatccctat
    11341 gaaattcgcg atcgcgggtt gatttatgcg caactggatt gcgaacacgt tgcgttgaac
    11401 gatttaagtt atttcgttga acagtgtccg gaagacccga tcagcgaaat gatccgtgcg
    11461 caaataaata acatcgcgca taaacatatt gtgctgcatt aattaatcga cattttactc
    11521 aagattaagg cgatcctatg aaacaaaaag tggttagcat tggcgacatc aacgtagcaa
    11581 atgacctgcc gttcgtactg tttggcggta tgaacgtgct ggaatctcgc gatctggcga
    11641 tgcgcatttg cgagcactac gtaactgtga cccagaaact gggtatccct tacgtgttca
    11701 aagcctcttt tgacaaagcc aaccgctcct ccatccactc ttatcgtgga ccgggcctgg
    11761 aagaagggat gaaaatcttc caagagttga agcagacttt tggcgtgaaa attatcaccg
    11821 acgttcacga accaagtcag gcacagcccg ttgctgatgt cgtggatgtg attcagttgc
    11881 cggcgtttct tgctcgccag actgacctgg ttgaagccat ggcgaaaacc ggtgcggtaa
    11941 ttaacgtcaa gaaaccacag tttgtcagcc cgggacagat gggtaatatc gttgataaat
    12001 tcaaagaagg cggcaacgaa aaagtgattc tttgcgatcg tggtgctaac ttcggctatg
    12061 acaacctggt tgtcgatatg ctgggcttca gcattatgaa gaaagtgtct ggtaactcgc
    12121 cggtgatttt cgacgtgacc cacgcactgc aatgccgcga tccgtttggc gcagcttccg
    12181 gtggtcgtcg tgctcaggtg gctgagctgg cacgagccgg tatggcggta ggtctggcgg
    12241 ggctgtttat tgaagcgcat ccggatccgg aacatgcgaa atgtgatggt ccatccgcgc
    12301 tgccgctggc taaactggaa ccgttcctca agcagatgaa agcgattgat gatctggtga
    12361 aaggtttcga agaactggat accagcaagt aatctttttt gcttgaaaaa taaagtctta
    12421 gcgttctgcg ttaagacttt tttcatgggt gccagataca aaaaaggccg caggctgtta
    12481 cccctgcggc cggtttcggg cgcatattgc catcacggca gcctgacgcc cgttttcacc
    12541 ttacttccgg ttacgccacc agccgacaat cgctgcggta ataattcccg ccaggatcgg
    12601 tgctgccagg tcgtgccaga aaatcatggc aaactgcgcg agcgtcatat agccgccttg
    12661 ttgtaatgac aacattttgc ggctattctt gaattgttct ggttcaagat tagcccccgt
    12721 tctattgtca ggttaaacct ctcaacgtgc gggggttttc tctttccagc aaccaatgcc
    12781 accagggata aagcccccgc aacattgcgc ctcaccggat aacgccggct tgatgtggat
    12841 actacg
//
LOCUS       DAJTMC010000118        12783 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.118, whole genome shotgun sequence.
ACCESSION   DAJTMC010000118 DAJTMC010000000
VERSION     DAJTMC010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12783
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.118"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(<1..454)
                     /locus_tag="OO130_002962"
     CDS             complement(<1..454)
                     /locus_tag="OO130_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5366288.1"
                     /translation="MKKVFAKSLLVAAMFSVAGSALAVQKDITVTANVDAALDMTQTD
                     NTALPKAVEMQYLPGQGLQSYQLMTKIWSNDVTKDVKMQLVSPAQLVQSLDASKIVPL
                     TVTWGGEEIKADAATTFTATKIFASDALTNGSLAKNLMFAQTTKGVLET"
     gene            complement(484..1200)
                     /locus_tag="OO130_002963"
                     /pseudo
     CDS             complement(484..1200)
                     /locus_tag="OO130_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225871.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            1509..2285
                     /gene="exuR"
                     /locus_tag="OO130_002964"
     CDS             1509..2285
                     /gene="exuR"
                     /locus_tag="OO130_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HCR5366289.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            2630..3292
                     /locus_tag="OO130_002965"
     CDS             2630..3292
                     /locus_tag="OO130_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5366290.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            3296..3679
                     /gene="mzrA"
                     /locus_tag="OO130_002966"
     CDS             3296..3679
                     /gene="mzrA"
                     /locus_tag="OO130_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HCR5366291.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            3826..4194
                     /locus_tag="OO130_002967"
     CDS             3826..4194
                     /locus_tag="OO130_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
                     /protein_id="HCR5366292.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHHKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            4232..4537
                     /locus_tag="OO130_002968"
     CDS             4232..4537
                     /locus_tag="OO130_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HCR5366293.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            4540..4944
                     /locus_tag="OO130_002969"
     CDS             4540..4944
                     /locus_tag="OO130_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HCR5366294.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            4934..5233
                     /locus_tag="OO130_002970"
     CDS             4934..5233
                     /locus_tag="OO130_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HCR5366295.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            5419..5811
                     /locus_tag="OO130_002971"
     CDS             5419..5811
                     /locus_tag="OO130_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCR5366296.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSKFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            5881..6784
                     /locus_tag="OO130_002972"
                     /pseudo
     CDS             5881..6784
                     /locus_tag="OO130_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
     gene            7077..7442
                     /locus_tag="OO130_002973"
     CDS             7077..7442
                     /locus_tag="OO130_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCR5366297.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEP"
     gene            7684..8040
                     /locus_tag="OO130_002974"
     CDS             7684..8040
                     /locus_tag="OO130_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCR5366298.1"
                     /translation="MQWYLAVLKNYVGFSGRARRKEYWMFTLINAIVGAIINVIQLIL
                     GLEFPFLSLIYLAATIIPVIALCVRRLHDTDRSGVWALLYLVPIIGWLVLFVFACLEG
                     NSGSNRYGNDPKFGSN"
     gene            complement(8091..8983)
                     /gene="yhaJ"
                     /locus_tag="OO130_002975"
                     /pseudo
     CDS             complement(8091..8983)
                     /gene="yhaJ"
                     /locus_tag="OO130_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
     gene            9088..9789
                     /locus_tag="OO130_002976"
     CDS             9088..9789
                     /locus_tag="OO130_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HCR5366299.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQQLIASPEGTMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            9812..9976
                     /locus_tag="OO130_002977"
     CDS             9812..9976
                     /locus_tag="OO130_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5366300.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(10110..11420)
                     /locus_tag="OO130_002978"
     CDS             complement(10110..11420)
                     /locus_tag="OO130_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HCR5366301.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNS
                     VVFTQQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(11448..12780)
                     /locus_tag="OO130_002979"
                     /pseudo
     CDS             complement(11448..12780)
                     /locus_tag="OO130_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
BASE COUNT         3284 a         3245 c         3033 g         3221 t
ORIGIN      
        1 ctgtctccag aacacctttg gtggtttgag caaacatcag gtttttagcc agagaaccgt
       61 tagttaacgc atcggaagca aagattttgg ttgcggtaaa agtggttgcc gcatcagctt
      121 taatttcttc accgccccag gttactgtca gcggaacaat cttgctagca tccaggcttt
      181 gaaccagttg cgccggggaa actaactgca tttttacatc tttggtaacg tcgttagacc
      241 agattttggt catcaactgg taagattgca gaccctgccc cggcagatat tgcatttcta
      301 ctgctttcgg caacgcggtg ttatcggtct gtgtcatatc cagagcggca tcaacgttgg
      361 ccgttacagt aatatccttt tgtactgcca atgcggagcc tgcaacagaa aacatcgctg
      421 cgaccagaag agattttgca aatacctttt tcattactat tccttcgttt attacaatga
      481 taattaatgg accgccagtt caaactcgcc attatctttg gtgatccagt tattatattt
      541 gacaacgatt ttgtcgtagc cagatatgct ttcaagattt ctttcctgac cagggaagat
      601 gtttttattt acggtctgta tcttacaatt ttctttgcta ttatttttac aataagcgat
      661 cgttaaaatc tttaagcgtt gattccctgc attaactaat ttttgggcag catttacttc
      721 taacttagca ataggttgct gaggtggaac actaacaaca atcccccaaa ttaagctaac
      781 agttaactgc atagagaaat cttttttatc tgtggcaacg tcatcaatac tgggaaccgc
      841 ttcaaattta acgcgttaat ttttttcttt ttgctctggt tccattgcaa caaagcgcac
      901 agttttgcta ctaccggctg gtaaagcgaa tttaggtggc ataacaacca gatgatttgc
      961 atcaccggat ttaatctcaa cttcattttc ctgaggcgtc gatggattat cgatacggaa
     1021 tactgtcgcc ttgatgtact gaacttcatt acttttagat attacgcgaa cgttgccttc
     1081 atcctgacta ttaatggaaa cagccatagg ataaacagtc atatttgact gtgccaaaaa
     1141 aggagcgaac acgaataaaa gcggcgcaag ccgtgataag agattaattt tcgccaccat
     1201 ataaatttga tcttacaata ttgtttccaa aaagttggtg tattagagat gtagatttat
     1261 tgtaagtcaa tataaaaaag catttttagt taatattaaa tttaaaggct gggaattaac
     1321 ttgctgaaat ttagtagctt tcacaatgac gatacttatt ttttaggaat ttataaatat
     1381 tcacaatatt aattcaatac attaattcaa tacattaatt caatcaccaa atttatatat
     1441 gaccaatggc atacccatga ataaagtggt atgataactt cgtccgcatt ccactccggg
     1501 agacgcatat ggaaatcact gaaccacgcc gtttgtatca acaacttgcc gctgacctga
     1561 aagagcgcat tgaacagggc gtctatctgg tgggtgataa actgcctgcc gaacgcttta
     1621 ttgccgatga aaagaacgtc agccgaacgg tagttcgtga agccatcatc atgctggaag
     1681 ttgaaggcta tgtggaggtg cgtaaaggtt caggtattca tgtggtttcc aaccagccgc
     1741 gccatcagca ggcggctgac aataatatgg aattcgccaa ttacggtccg tttgagttgc
     1801 ttcaggctcg ccagctcatc gaaagtaata ttgccgagtt cgcggcaact caggtaacga
     1861 aacaagacat catgaaactg atggccattc aggaacaggc gcgcggcgaa caatgcttcc
     1921 gtgattccga gtgggattta cagttccaca ttcaggtagc cctggcgacg cagaactccg
     1981 ccctggcggc catcgttgaa aaaatgtgga cccagcgtag ccataacccg tactggaaaa
     2041 aactacacga acacattgat tcccgtaccg ttgataactg gtgtgatgac cacgatcaaa
     2101 ttctcaaggc gttaattcgc aaagatcccc atgctgctaa gctggcaatg tggcagcatc
     2161 tggaaaacac caaaatcatg ttatttaacg aaaccagcga cgatttcgag ttcaatgccg
     2221 accgctatct attcgccgaa aacccggtag tgcatctcga tacggccacc agtggcagta
     2281 aatgaaactc ctgtccgaca ggcgctgcga cgcgcctgtt cgcttcgtcg gtaagcgaaa
     2341 agtataaagt gtcagcctgt gtaaatcctc tcgccaccct cccctgcatt cagcaaaatc
     2401 agactccacg gacatgtaat tttgataacg aacaacgttg acctttgtta caattagatt
     2461 caatttgaat ttatgttttt gaatgctttc ttatctcacg atttaacagg gaatagttca
     2521 ggctgtgttg atgtatcaaa cccgcagaac ataccaaaac agcaataaca ttgcggtagt
     2581 gcatctttta aaaccagcgt ggcgttaacc gattcaccag gaataatgaa tggaactttt
     2641 gacccaattg ctgcaagccc tgtgggcgca ggattttgaa accctggcca atccatcgat
     2701 gattggcatg ttgtattttg tcttgtttgt aattttgttc cttgaaaacg gcttgcttcc
     2761 agcggccttt ttaccgggcg acagtttact ggtattggtc ggcgtgttga ttgcgaaagg
     2821 cgcgatgggt tacccgcaaa cgattctgct gctgaccgtt gccgccagcc tcggctgctg
     2881 ggtcagctat attcaggggc gatggctggg caatacccgc accgtacaaa actggctatc
     2941 tcatttaccc gcgcattatc atcaacgcgc acaccatctt tttcataaac acggtttatc
     3001 ggcgctgtta attggtcgct ttattgcgtt tgtcagaaca ctgctgccga cgattgccgg
     3061 gttatcaggg ctgaataacg cgcgctttca gtttttcaac tggatgagcg gtctgctgtg
     3121 ggtattgatc ctgacaactc tgggttacat gctcggcaaa acgccggtat ttttaaagta
     3181 cgaggaccag ctgatgtcat gcctgatgct gctcccggtg gtgctgctgg tgtttggcct
     3241 ggcaggttct ctggtcgtgt tatggaaaaa gaaatatgga aatcgggggt aagggatgca
     3301 aatacctcgc atgtcgcttc gccagctagc ctggtccggc actgttttac ttctggtcgg
     3361 cacgctgttg ctggcctggt ccgcggttcg ccagcaagag tctacgctgg cgattcgtgc
     3421 cgttcatcaa ggcacaacga tgccagacgg tttttcgatc tggcatcacc ttgacgctca
     3481 tggcattcct ttcaaaagta tcacccccaa aaacgacact ctgttaatta catttgactc
     3541 cagcgaccag agcgccgccg caaaagcggt cctcgacaga acattgcccc atggctacat
     3601 cattgcgcag caggacaata acagtcaggc tatgcagtgg ctgacccggt tacgggataa
     3661 ctctcatcgc ttcggataac ttcctgttat ccgaaacata tcactcactt tggtgatttc
     3721 accgtaactg tctatgatta atgaggcggt ggttaacact accgcttcgt tgtctggaca
     3781 atcgttcctt tgtaacaggt ccgaatcaca atggaaggtt caagaatgaa ataccgcatc
     3841 gctttagctg tttctctctt tgctcttagt gccggtagtt atgccactac cctgtgtcag
     3901 gaaaaggagc aaaatatcct taaggagatc agctatgccg aaaaacacca aaaccagaat
     3961 cgtattgacg gtctgaataa agccctgagt gaagtccggg ccaactgttc agatagccag
     4021 ctgcgtgccg atcatcacaa gaaaatcgca aagcagaaag atgaggtggc ggaacgccag
     4081 caagatttag ccgaggcgaa gcaaaaaggc gatgccgata agattgccaa acgcgaacgg
     4141 aaactggcag aagcgcagga agagctgaaa aagctggaag cgcgcgacta ctaactcaca
     4201 atagtcacta cttactcacc tggagaaaac tatgtcgaaa gaacacacta cggaacatct
     4261 gcgtgctgag ttgaaatccc tttccgatac gctggaagag gtgcttagct catctggcga
     4321 gaagtcgaaa gaagagttga gtaagattcg tagcaaagcg gagcaggcac tgaaacagag
     4381 ccgttatcgc ctgggtgaaa ccggtgatgc catcgccaaa caaacccgtg tcgcggcggc
     4441 gcgtgctgat gagtatgtgc gtgaaaatcc gtggaccggc gtgggaattg gcgctgcaat
     4501 cggtgtagtg ctcggcgttc tgctgtcgcg tcgttaatta tggcggacac tcatcacgcg
     4561 caagggcccg gtaaaagcgt tctgggcatc gggcagcgaa ttgtttccat catggttgaa
     4621 atggtggaaa cacgtctgcg gctggcggtg gtggagctgg aagaggaaaa agctaatctc
     4681 tttcaacttt tactgatgct gggcctgacg atgcttttcg ctgcatttgg tcttatgagc
     4741 ctgatggtgc taattatttg ggcggttgat ccgcaatatc gcctgaatgc gatgattgcc
     4801 accaccgtgg tgttgctgct actggcactg attggcggta tctggacgtt acgtaaatcg
     4861 cgtaaatcca ccttgctgcg ccatacacgc catgagctgg caaacgatcg gcaactgctc
     4921 gaggaggagt cccgtgagca gtaaagtcga acgtgaacga cgtaaggcgc aactgcttag
     4981 ccagatccag caacaacggc tggatctttc cgccagtcgt cgtgaatggc tggaggcaac
     5041 aggcgcttac gatcgtcgct ggaatatgct gctaagtctg cgctcctggg cgctggttgg
     5101 cagtagcgtg atggcgatct ggacgattcg ccatcctaat atgctggtcc gctgggccag
     5161 acgcggtttt ggcgtatgga gcgcctggcg tctggttaaa acgaccctca agcagcaaca
     5221 gcttcgcggt taacttccct ctggccggag ccattccggc cctatcactc aaattttttg
     5281 aagatttttg acagttttcc ttgctaacaa tcatcattca ccacgtttat gattctctcc
     5341 atcgacagca acgacgctaa taccgcgccc ttgcacaaaa aaacaatcag cagcctgagt
     5401 ggcgcagtgg agagtatgat gaaaaaatta gaagatgttg gtgtactggt agcgcgcatt
     5461 ttaatgccga ttctgtttat taccgcaggc tggggaaaaa tcaccgctta ctctggtacc
     5521 caacaatata tggaagcaat gggtgtcccg ggttttatgc tgccgctggt gattctgctc
     5581 gagttcggtg gtggtctggc aatcctgttc ggtttcctga ctcgcaccac agccctgttt
     5641 actgcgggct ttacgctgct gacggcattt ttatttcaca gcaagtttgc tgaaggcgtc
     5701 aactcgctga tgttcatgaa aaacctgaca atttctggcg gattcctgct gctggcaatt
     5761 accggtccgg gcgcgtatag catcgaccgc ctgctgaata aaaagtggta agcacgcgct
     5821 atacttaacg ataaaaaacg aggaggaagc tcctcgtttt tgctattgga ggagagaaaa
     5881 atggggcaac tgattgacgg cgtctggcat gacacctggc cccactggca caggcggttt
     5941 tatcgcagag aaagatcgtt atcatctcta tgtttcactc gcctgcccgt gggcgcaccg
     6001 cacgctgatc atgcgcaaac tcaaaggact ggaaccgttt atttccgttt ccgtagtgaa
     6061 cccgctgatg ctggaaaacg gctggacctt tgatgacagt tttccgggag caaccggaga
     6121 tacgctctat caacatgaat ttctgtatca gctttatctc cacgccgatc cacactacag
     6181 cggacgagtt actgttcccg tgctgtggga caaaaagaac cacaccattg tcagcaacga
     6241 atcagcagaa atcatccgca tgttcaatac cgcgtttgat gcgctgggcg caaaagcggg
     6301 tgattactac ccaccagccc tgcaaacgaa aattgacgaa cttaacggct ggatttatga
     6361 caccgttaac aacggcgtgt ataaagctgg ttttgccacc agccagcaag cctacgacga
     6421 ggcggtggcg aaagtgtttg aatcgctggc gcgactggag cagattttag gccagcatcg
     6481 ttacctgacc ggcaaccagc taaccgaagc cgatattcgc ctgtggacta cgctggtgcg
     6541 ttttgatcca gtgtatgtga cccacttcaa gtgtgataag caccgcatca gcgattacct
     6601 gaatctgtat ggcttcctgc gcgatatcta ccagatgccg gggatcgccg aaactgtcaa
     6661 tttcgatcac atccgtaatc attacttccg cagccataag accatcaacc ctacggggat
     6721 tatttcaatt ggtccgtggc aggatctcga tgaaccgcat ggacgagatg ttcgcttcgg
     6781 ttaaaaaaag gcaccttacg aggtgcctct gataattagc taagtgcatg ttgttcatgt
     6841 cggatgcggc gtgaacgcct tatcaaacct acaaaacgtt tatattcaat atgttaaaat
     6901 gaccgcgtag gccagataag cgtagcgcat ctggcgattt tgcgcttgtc atcagtctta
     6961 gcgcaccttc acggcacttt attaattcct tgaacgaata tttactgcca tttcattcac
     7021 gtctattctt aatttgctgc tttaaaaaca agtgattgag caaaattgag gcaaaaatgg
     7081 actggtatct gaaagtacta aaaaattatg tcggtttccg gggtcgtgcg cggcgcaaag
     7141 agtactggat gtttattctg gtcaacatca tctttacgtt cgtgctgggg ctgctggata
     7201 aaatgttagg ctggcaacgc gccggtggcg aaggcatcct gacgacaatc tacggtattc
     7261 tggtgttttt accgtggtgg gcggttcagt tccgccgcct gcacgacacc gaccgctcgg
     7321 cgtggtgggc actgttgttc ttaatcccgt ttatcggctg gcttattatc atcgtcttta
     7381 actgccaggc gggtacgcca ggcgaaaacc gctttggacc agatccgaaa ctggaacctt
     7441 aaaacaaatc tgctcgccgg atgtctgact catccggctt ttatcatcat gttgatgaat
     7501 gaataattta ctaatgttta tttaaaatat ttcaatgtcg ttatataaat tacctataaa
     7561 aaacaaccat gtatttatct taaatatcaa aacctttata acatccgaaa aagaaagata
     7621 cgaatagcat ttacacaata cccggaatag actttcgtca cttttaatta aagggatgtt
     7681 tttatgcagt ggtatttagc cgtattaaaa aattatgttg gtttctctgg tcgcgcacgt
     7741 cgtaaagagt actggatgtt tactctgatt aacgccatcg tcggcgctat tatcaatgtc
     7801 attcaattga ttttaggttt agagtttcca ttcctttctc tcatttacct ggcagcaaca
     7861 atcattccgg tcattgctct gtgtgttcgt cgtctgcacg atactgaccg ttcaggtgtg
     7921 tgggcgcttc tctatctggt accgatcatt ggttggttag ttctgtttgt atttgcctgt
     7981 ttagaaggca attctggcag caatcgttac ggaaacgatc ctaagtttgg ttcaaattaa
     8041 tcttagaatt ggggcgatat ttcgcccctt tttattaaca ataataaata ttattttccg
     8101 ctaaaaagtt tgggaatttc ccgcagacac caggatttcg cttcccccat actgtcacga
     8161 cgccaggcca taataatatc gatctcgctg gtcgattccg ggctgacgac acgcaaccgc
     8221 ccttccgcaa tatctttttc gaccatcgga tacggcatcg tcgccacgcc aagccccgcc
     8281 agtaatgcct gacgtttatc ttcaatcgtg ctcaccgtta agcgcggctg tttgtccagc
     8341 agctgtacgg tcaacaccgg gcgctcacga gcggtatccg ctaccgcaat tccacgatat
     8401 ttcacgcgcg tcacttcaga taacggttcc ggctcctgat gaatcgggtg atcaggcgcg
     8461 gcaacgtaga cgttcattaa ggtatagagc ttgcgcgagt tgatctccga cgaggaacga
     8521 aaatgcatat ccggcgcgat aacgatatcc gcccgcccct gctccagtcg ttcccacgcc
     8581 cccgccagca cttcggtgat gattgccagt tgggtattgg cttttgccgc cagtttgtcg
     8641 attaacggga aaaaggcagg tgtcggtacc agcgcctcgg tcacaatggt gagatgcgtt
     8701 tcccaaccgc gcgcgagggc ttccgcatcg gtagtcagtt tatctgctgc ttccagcaaa
     8761 acgcgccctc gctccagcaa catcctcccg acattggtga atttggtgcg atggcccgag
     8821 cggtcaaaca gcaccacatc cagctcttct tccagttttt gcatggtgta gctaagtgcg
     8881 gaaggcacgc gtcccagctc atccgccgcc gccgcaaaac tgccccgacg atcgcatcca
     8941 taacccgtag tgcttccagc gttaatgccc tttctttggc catttcgttc tcattcagga
     9001 aatttgaaca taccgggcag aatatctggc taacaatgta gcgtccagcc ccttaccata
     9061 aaaggaagta aagagaggtc aagaattatg attactaccc gaactgccag gcagtgtgga
     9121 caagcagact acggatggtt gcaggcccgg tatacttttt cctttggaca ctacttcgac
     9181 ccgaaattgt taggctatgc ctccctgcgt gtgcttaacc aggaagtgct ggccccaggt
     9241 gccgcctttc agccgcgaac ctatcccaaa gtcgatattt taaatgtgat tctggatgga
     9301 gaagcagagt atcgcgacag cgaaggcaat catgttcagg ccagcgccgg tgaggcgttg
     9361 ctgctctcta cccagccggg tgtcagctac agcgaacaca atctcagcaa agacaaaccg
     9421 ttaacacgaa tgcagctttg gctggacgcc tgcccgcagc gagagaatcc gctgattcaa
     9481 aaactggcgc ttaatatggg caagcagcaa ttaatcgcct cgccagaggg tacgatggga
     9541 agcctgcaat tacgccagca agtgtggctg caccatatcg tgctcgacaa aggcgaaagt
     9601 gcgaatttcc agttgcatgg gccacgcgcg tatttgcaat cgattcacgg gaaatttcat
     9661 gcgcttacgc atcatgaaga gaaagcggcg ctgacctgcg gtgatggggc gtttattcgt
     9721 gacgaggcta acattacgct ggttgccgat tccccactgc gcgctttgct gatagatttg
     9781 cctgtctagt tgtttttaca gggagatgat gatgagtaaa aaatcggcca aaaagcgcca
     9841 gccagtgaag cccgtggtgg cgaaagaacc tgctcgcacc gccaaaaatt ttggctatga
     9901 agagatgttg agcgagctgg aagccatcgt cgcggatgct gaaacgcgtt tagccgagga
     9961 tgaagctacc gcgtaaagac aggttgccgg atgcggcgaa aacgccttat ccggcctaca
    10021 aaatccaaca acattaacga attaaacaac ccgtaggccg gataaggtgc gccagcatcg
    10081 catccggcac aatactcaaa acctggcgtt tatctggcct tgctcgccat aatctcgata
    10141 atctgccgat ccgtttgctg catcgaatgg cttgctaacg cacacaggtt ggcaatcgac
    10201 tgctcaacat catgcgccac gatcccttca ttgccggtca cggcggtatc atccagcgcc
    10261 attaacaccg ctttccacgc agccgaagca ctggtcgaaa ccttcatcgc gcagctgttc
    10321 gacgcaccat cgcaaatcat gccgctgaca tcgccgatca tactgctgat cgccatcgag
    10381 atagtttcat aacgcccatc caccagccat gccatcccgg cggcggcccc cattgctgcg
    10441 gtcgttgcgg cacaaagtgc agacaaacgc ggtaactggt tatggatgta aattgcgctc
    10501 aaatgagaaa gcatcagcgc acgcgccagt cgttcatcat ccgctccgaa gtgttctgct
    10561 acaaccacca caggcattgt tgcggtgatc ccctggttac ccgagccgga gttactcata
    10621 gccggaagcg tagcgccgcc catacgcgca tcggatgccg cgctggtacg aatcacaatg
    10681 gatgaagaga gatctttcgc cagcaaaccg cgctcgcact gtttttccag cgtcgcgcca
    10741 atatgcagcc cccacttacc gctcaaacct tcctgcgata acgcacaatt tagcttcgcg
    10801 gaatcgagaa taaagcggat cgccgcaaac gggacttcat tgacgaactt caggatctca
    10861 gccagcgtcg ttctggaaag caccgtcagc ggagactctt gctcgccctc tgccacacac
    10921 gcctgctggg taaacaccac actattgtgc gtctcaatat gcacaatgtt ggtatgcccg
    10981 ccgacgatgg tgacacacgc ccacttctca ccgttccaga ctttggcgcg tgagaagagg
    11041 atttcattgc aaggttcctg gatcttaacg gagactttcc ccgccgccag cagtgctttg
    11101 gcatcggcaa ttgcctgcgc agttgcgtct ttcagcactt ccagcccggc gttggcattt
    11161 ccacctaacg cccccagcgc cgccgcaatc ggcagcccca ccattcccgt gccgggaacg
    11221 gtgacgccca gaccgttctt catcagattt ggcgaaaccc aggcttctac acgttcaacc
    11281 ggaccttcca gttctgccgc agcaaccgcc gccgccagcg ccagtgaaat cggttcagta
    11341 catcccagcg ccggttttac ttcctcctga acggcgagga tgtaacgctg ccataacgga
    11401 tttaaagtcg aatcaaacat attaaaaacc ttaaaatttc aggtaaatca agaaaatgcc
    11461 aggaacggag aaacacaaag caacaaacca gtgacgataa tcaggtacag agacatccct
    11521 ttgtatttgt gcaatgccgg tactttgtaa accagccacg ccgggatcag gcaccctacc
    11581 atgccgaaaa tcgggctaca gatagaggtg aagctcaaca ccggtgcgtt cagtacgatg
    11641 gcgctccagg ccagcaaaat ggcgaaaatc atgatgccgc gctgaacaag attttcgtta
    11701 atcttctcgg caggcatctt gcgacgcagg atgttcatta cgatcccttg cgttgcttcg
    11761 cgaaagccta aatagacgcc aaagaatgca gtcatgacag caaagatatt gagaatgacg
    11821 ctgaccactt tcacccacgc tgcgccgtca ccgctaataa actgtgcggc aatcgccagc
    11881 gcggaaatat tctgctcgta ggcttttacc gcttcgtcat gtcccatcgc cagcgtgaac
    11941 gacacggcgt agaaaaagac ggtgacaaat aaaatgccaa acgcgatatt cattgcccgc
    12001 aatgctttat gccgcgccac ttcaattgat ttttcccgcg agcgataaga gatcaccatc
    12061 gggcttaacg tctggataaa cagaatcgac gtcagggtaa acggcagcgt aataatggcg
    12121 tttttcacca gcagccccag cggcggtagc gaaccgacgt tatacagatg ccacattccc
    12181 accatcgaca cgcccagcgc agcgactacc agcagcttgg tcagcaccat gccggtcgaa
    12241 attttgaata acaatttctc gccgcgtgaa gagatcgcca ccagaatgca aatcagtacc
    12301 agaccataaa agggactgtc tgacagcacc ccttccgtca cgccgaaggt atgcagatag
    12361 gaagcactat cgttggtgat ggcggtggaa tagacgaaca tccaaatcac cagcattacg
    12421 aaatagagcg cacctaacag gatgccccag tttttaccta aataaccgct aatgacactc
    12481 gggtaatctt tacattctgt gtgattctgc cagcgtatta ataaacaacc gctgaaacag
    12541 atacattgcc gggtaaccaa tcaccgatga gagcaaaaat acccacaatc ccatcaaacc
    12601 gacctgcacc gggagaaaaa caatccccgc gccaatcgcc atcccgatac tcataatcac
    12661 ccagccggtg tcagtactgt cgaatttgat cgcttctcgc cactcgctct cactcattcc
    12721 agcacgaccc gccggggtcg aagcgtctgc aatgacgcct ttattcgatg caatttccat
    12781 aat
//
LOCUS       DAJTMC010000119        12782 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.119, whole genome shotgun sequence.
ACCESSION   DAJTMC010000119 DAJTMC010000000
VERSION     DAJTMC010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12782
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.119"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            47..520
                     /locus_tag="OO130_002980"
                     /pseudo
     CDS             47..520
                     /locus_tag="OO130_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001589353.1"
                     /note="type IV secretion system coupling protein; similar
                     to F plasmid TraD; incomplete; partial in the middle of a
                     contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraD"
     gene            complement(529..927)
                     /gene="vapC"
                     /locus_tag="OO130_002981"
     CDS             complement(529..927)
                     /gene="vapC"
                     /locus_tag="OO130_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(fMet)-specific endonuclease VapC"
                     /protein_id="HCR5366302.1"
                     /translation="MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIIVTNNTREFERVGGLRTEDWS"
     gene            complement(927..1154)
                     /gene="vapB"
                     /locus_tag="OO130_002982"
     CDS             complement(927..1154)
                     /gene="vapB"
                     /locus_tag="OO130_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HCR5366303.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAG
                     ETWDEWFDGHSVSADFMDNREQPGMQERESF"
     gene            1236..1655
                     /locus_tag="OO130_002983"
     CDS             1236..1655
                     /locus_tag="OO130_002983"
                     /inference="COORDINATES: protein motif:HMM:NF020332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative relaxase"
                     /protein_id="HCR5366304.1"
                     /translation="MMSIAQVRSAGSAGNYYTNKDNYYVLGSMGERWAGQGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNRHRPGYDLTFSAPKSVSMMAMLGGDKRL
                     IEAHNQAVDFAVRHHPCGGTGLHTDDDGRTVRNGADR"
     gene            1642..6501
                     /gene="traI"
                     /locus_tag="OO130_002984"
     CDS             1642..6501
                     /gene="traI"
                     /locus_tag="OO130_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005079803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HCR5366305.1"
                     /translation="MLTGNLVMALFNHDTSRDQEPQLHTHAVVTNVTQHNGEWKTLSS
                     DKVGKTGFIENVYANQIAFGRLYREKLKEQVEALGYETEVVGKHGMWEMPGVPVEAFS
                     GRSQTIREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAEWMQTLKETGFDIRAYRD
                     AAEQRAYTRTQTPGPASQDGPDVQQAVTQAIAGLSEFMYTDLLARTVGILPPENGVIE
                     RARAGIDEAISREQLIPLDREKGLFTSGIHMLDELSVRALSRDIMKQNRVTVHPEKSV
                     PRTAGYSYAVSVLAQDRPSLAIVSGQGGAAGQRERVAELVMMAREQGREVQIIAADRR
                     SQMNLKQDERLSGELITGRRQLLEGMAFTPGSTVIVDQGEKLSLKETLTLLDGAARHN
                     VQVLITDSGQRTGTGSALMAMKDAGVNTYRWQGGEQRPATIISEPDRYVRYARLAGDF
                     AASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVLGHPEVTMTALSPVWLDSRSRY
                     LRDMYRPGMVMEQWNPETRSHDRYVTERVTAQSHSLTLRNAQGETQVVRISSLDSSWS
                     LFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASVSEDAMTVVVPGRAEPATLPVS
                     DSPFTALKLENGWVETPGHSVSDSATVFASVTQMAMDNATLNGLARSGRDVRLYSSLD
                     ETRTAEKLARHPSFTVVSEQIKARAGETLLETAISLQKAGLHTPAQQAIHLALPVLES
                     KNLAFSMVDLLTEAKSFAAEGTGFADLGGEINAQIKRGDLLYVDVAKGYGTGLLVSRA
                     SYEAEKSILRHILEGKEAVTPLMERVPGELMEKLTSGQRAAARMILETSDRFTVVQGY
                     AGVGKTTQFRAVMSAVNMLPESERPRVVGLGPTHRAVGEMRSAGVDAQTLASFLHDTQ
                     LQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIAAGGGRAVASGDTDQLQAIAPG
                     QPFRLQQTRSAADVVIMKEIVRQTPELREAVYSLINRDVERALSGLERVKPSQVPRLE
                     GAWAPEHSVTEFSHSQEAKLAEAQQKAMLKGEAFPDVPMTLYAAIVRDYTGRTPEARE
                     QTLIVTHLNEDRRVLNSMIHDAREKAGELGQVQVMVPVLNTANIRDGELRRLSTWENN
                     PDALALVDNVYHRIAGISKDDGLITLQDAEGNTRLISPREAVAEGVTLYTPDTIRVGT
                     GDRIRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQQTRVIRPGQERAEQHIDLAYA
                     ITAHGAQGASETFAIALEGTEGNRKLMAGFESAYVALSRMKQYVQVYTDNRQGWTDAI
                     NNAVQKGTAHDVFEPKPDREVMNAERLFSTARELRDVAAGRAVLRQAGLAGGDSPARF
                     IAPGRKYPQPYVALPAFDRNGKSAGIWLNPLTTDDGNGLRGFSGEGRVKGSGDAQFVA
                     LQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGEGRPWNPGAITGGRVWGDIPDN
                     SVQPGAGNGEPVMAEVLAQRQAEEAIRRETERRADEIVRKMAENKPDLPDGKTEQAVR
                     EIAGQERDRAAITEREAALPESVLREPQRVREAVREVARENLLQERLQQMERDMVRDL
                     QKEKTPGGD"
     gene            6521..7267
                     /gene="traX"
                     /locus_tag="OO130_002985"
     CDS             6521..7267
                     /gene="traX"
                     /locus_tag="OO130_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HCR5366306.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSFLVWSPGQRDIIKTVALVLM
                     VLDHINLIFQLKQEWMFLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSNRLYRAEDRAERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVLC
                     AGKSLPRFWPGDFFPTFYACHLAVLGVLAL"
     gene            7322..7882
                     /locus_tag="OO130_002986"
     CDS             7322..7882
                     /locus_tag="OO130_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HCR5366307.1"
                     /translation="MTEQKRPVLTLKRKTEGTATVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAGARLAKIRRQNRIKAELQAVLDEK"
     gene            8013..8225
                     /locus_tag="OO130_002987"
     CDS             8013..8225
                     /locus_tag="OO130_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HCR5366308.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGGRAVILEVSGRWMEAAEAWRRAAC
                     VAPRTDWQQFARKRAEHCHRRCRGRG"
     gene            8469..8930
                     /locus_tag="OO130_002988"
     CDS             8469..8930
                     /locus_tag="OO130_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HCR5366309.1"
                     /translation="MRKYIPLVLFIFSWPVLSADIHGRVVRVLDGDTIEVMDSLKAVR
                     IRLVNIDAPEKKQDYGRWSTDMMKSLVAGKTVTVTYFQRDRYGRILGQVYAPDGMNIN
                     QFMVRAGAAWVYEQYNTDPVLPVLQNEARQQKRGLWSDADPVPPWIWRHRK"
     gene            8976..9185
                     /gene="hha"
                     /locus_tag="OO130_002989"
     CDS             8976..9185
                     /gene="hha"
                     /locus_tag="OO130_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HCR5366310.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLSNSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
     gene            9224..9814
                     /locus_tag="OO130_002990"
     CDS             9224..9814
                     /locus_tag="OO130_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HCR5366311.1"
                     /translation="MKLIIFILIVLIIAALLIRIILRSVNQHSPLLMQLHAAGIRTGD
                     AERILSSGEYWQRQTTLLTEREVSFMKGLFRIVDMTRWYLCPQVRVADIVQLNGNIRP
                     RSRQWWQLFRMVSQWHVDVVIVELRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQ
                     AGIPLLRSHDARKLLQMTGEWLNTTGADQQSPEHRS"
     gene            10054..10314
                     /gene="repA"
                     /locus_tag="OO130_002991"
     CDS             10054..10314
                     /gene="repA"
                     /locus_tag="OO130_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HCR5366312.1"
                     /translation="MSQTENAVTSSLSQKRFVRRGKPMTDSEKQMAAVARKRLTHKEI
                     KVFVKNPLKDLMVEYCEREGITQAQFVEKIIKDELQRLDILK"
     gene            10538..10612
                     /gene="tap"
                     /locus_tag="OO130_002992"
     CDS             10538..10612
                     /gene="tap"
                     /locus_tag="OO130_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HCR5366313.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            10605..11462
                     /gene="repA"
                     /locus_tag="OO130_002993"
     CDS             10605..11462
                     /gene="repA"
                     /locus_tag="OO130_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HCR5366314.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGARTLPFCGKLMEKAVGFTSR
                     FDFAIHVAHARSLGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLAELGLITYQTEYDPLIGCYIPTDITFTSALFAALDV
                     SEEAVAAARRSRVEWENRQRKKQGLDTLGMDELMAKAWRFVRERFRSYQTELKSRGMK
                     RARARRDADRQRQDIVTLVKRQLTREISEGRFTASREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            complement(12524..>12782)
                     /locus_tag="OO130_002994"
     CDS             complement(12524..>12782)
                     /locus_tag="OO130_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000484486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCR5366315.1"
                     /translation="AAAFLGVVPVEKYSGTSVRGCPKLSKIGPPEIRAKLYLASLCGL
                     RFNPLMKAMYERLCLRGKAKMCAIGALMRKLVHWCYGVLKT"
BASE COUNT         3104 a         3223 c         3784 g         2671 t
ORIGIN      
        1 ttaaaatcac catcatacac taaatcagta agttggcagc attaccaaat atcaggaacg
       61 accgaaggtc gctccggagt ttattccgcg tgacatcaac ccggaaatgg agaaccgtct
      121 gagtgccgta cttgccgcaa gggaagcaga aggtcgtcag atggccagcc tcttcgaacc
      181 ggatgtcccg gaggttgttt ccggagaaga cgtgactcag gctgaacagc cgcaacagcc
      241 ggtgtctcct gtcatcaacg ataagaagtc agattcaggt gtgaatgttc cggcaggggg
      301 gatcgagcag gagctgaaaa tgaaaccgga agaagagatg gaacagcaac tgccacccgg
      361 gatcagtgaa tccggtgaag tggtggatat ggccgcttat gaggcatggc agcaggaaaa
      421 tcatccggac acctggcagc agatgcagcg tcgtgaagag gtgaacatca atgtgcaccg
      481 ggaacgcggg gaggatgttg agccgggaga tgatttctga tgaacaggtc agctccagtc
      541 ttcagttctc aggccgccca cacgttcaaa ttcccgggtg ttattagtca caataatcag
      601 cccccgactg cgggcatgac ctgcgatcat ttgatcaaat ggcccgacag ggcgtccctg
      661 acgggcaagt tctgctctta tctggccggt gtgtgtggct gcagcagcgt cgtaatccag
      721 aacatcaatg cgggaaacaa atccctcgat cacagcgaga ttacgttcag gcatctggct
      781 tttttctgca ccatatatca gctccatcag ggtgaccgaa ctgatacaca ttctcccctg
      841 gttcaggtta aaacgctccc tgacgctggc gggtttgttc tttatcgtaa aaatgcagat
      901 gttggtatcg agcataaact tcagcatcag aatgactccc tttcctgcat gccgggctgt
      961 tccctgttat ccataaaatc ggcgctgacg ctgtgtccgt cgaaccattc gtcccatgtc
     1021 tctccggctg gcgtaatgat tctggttcgt ccgacagcaa tcacttcaac gcgttttacg
     1081 ttttccggca atgcaaccgc ttttggcagt ctgaccgctt ggctgcggtt gctgagaaat
     1141 acggtggttt ccattatttg cctccttatg tggatatgtc ttgtgtatat ccattgtggg
     1201 attgataccg ggatatgtca aagggatata cgtttatgat gagtatcgcg caggtcagat
     1261 cggccggaag tgccggtaat tattatacca acaaggataa ttactatgtg ctgggcagca
     1321 tgggagaacg ctgggccggc cagggggctg aacaactggg gctgcagggc agtgtcgata
     1381 aggatgtctt tacccgcctt ctggagggca ggctgccgga cggggcggat ttaagccgca
     1441 tgcaggatgg cagtaacagg catcgtcccg gctacgacct gaccttctcc gctcccaaaa
     1501 gtgtctccat gatggccatg ctcggggggg acaaacgtct tattgaagca cataaccagg
     1561 ccgtggattt tgctgtccgt catcacccgt gtggaggcac tggcctccac acggatgatg
     1621 acggacggac agtcagaaac ggtgctgacc ggtaatctgg tgatggcact gtttaaccac
     1681 gacaccagtc gcgatcagga accacagtta cacacgcatg cggtggtgac taatgttacg
     1741 cagcataacg gcgaatggaa gacactgagc agtgacaaag tggggaaaac ggggttcatt
     1801 gagaatgtgt acgctaatca gattgccttt ggcaggctct accgggaaaa actgaaagag
     1861 caggttgagg cgctgggcta tgaaactgaa gtggtcggta agcacggtat gtgggaaatg
     1921 ccgggtgtac cggtggaggc cttttccgga cgctcacaga ctatccggga ggccgtcggg
     1981 gaagacgcct cgctgaaatc ccgggatgtg gcggcgctgg atacgcgtaa atccaaacag
     2041 cacgtcgatc ctgaagtcag aatggccgag tggatgcaga cgctgaagga aaccgggttc
     2101 gacatccggg catatcgtga cgcagcggaa caacgggcat atacccgcac gcagacaccc
     2161 ggacccgctt cacaggacgg gccggatgtg cagcaggcgg tgacacaggc cattgccgga
     2221 ttaagtgagt ttatgtatac ggacctgctg gccaggacgg tcggcatact gccgccggaa
     2281 aatggtgtga ttgaacgggc acgcgccggt atcgatgagg ccatcagccg tgagcagctt
     2341 atccccctcg atcgtgagaa ggggctgttc acgtccggga ttcatatgct cgatgagctg
     2401 tcagtccggg cactcagtcg tgacatcatg aaacagaacc gggtgaccgt acatccggag
     2461 aaaagtgtcc cccggacggc cggttacagc tatgccgtca gtgttctggc acaggaccgt
     2521 ccgtcgctgg ccattgtgtc cgggcagggc ggtgcagccg ggcagcgtga gcgggtggct
     2581 gaactggtca tgatggcccg ggagcagggg cgggaggtgc agattatcgc tgctgaccgt
     2641 cgctcgcaga tgaacctgaa gcaggatgaa cggctgtccg gtgaactgat aaccggacgt
     2701 cgtcagttgc tggaaggcat ggccttcacg ccgggcagta ccgttatcgt cgaccagggc
     2761 gaaaaactct ccctgaaaga gacgttaacc ctgctggatg gtgccgcacg tcataacgta
     2821 caggtcctga taactgacag cgggcagcga accggtacag gcagtgcact gatggccatg
     2881 aaggatgccg gggtgaacac atatcgctgg caggggggag aacagcgacc ggccaccatc
     2941 atcagtgaac cggaccgtta tgtccgctat gcccggctgg ccggagattt tgcggccagc
     3001 gtgaaagccg gagaagagag cgtggcacag gtcagcggag tacgggaaca ggccatactg
     3061 acacaggcca ttcgcagtga gctgaaaaca cagggcgtgc tcggacaccc ggaggtaacc
     3121 atgaccgccc tgtcaccggt ctggctggac agccggagcc gttatctgcg ggatatgtac
     3181 cgcccgggaa tggtgatgga gcagtggaac ccggagacac gcagtcatga ccgctatgtg
     3241 acagaacgtg tgacggcgca gagtcacagc ctgaccctgc ggaatgcgca gggcgaaacg
     3301 caggtggtgc gtatttcttc cctggacagc agctggtcgc tgttccggcc ggaaaaaatg
     3361 ccggtggcag acggtgagcg tctgagggtg acagggaaaa ttcccggact ccgcgtctcc
     3421 ggcggtgacc gcctgcaggt ggcatccgtc agtgaagatg cgatgacggt tgttgtgccg
     3481 ggacgggctg aaccggccac cctgcctgtg agcgattcac cgttcacggc actgaagctg
     3541 gagaacggct gggtggaaac gcccgggcat tccgtcagcg acagtgcgac ggtttttgcc
     3601 tccgtcacac agatggcaat ggacaatgcc accctgaacg gtctggcccg cagtggtcgt
     3661 gatgtccggc tgtattcctc actggatgaa acccgtactg cggaaaaact tgcccgccat
     3721 ccttccttta cggtggtttc tgagcagata aaggcgcgtg ccggtgagac attgctggaa
     3781 accgctatca gtctgcagaa agccgggctt cacacgccgg cacagcaggc cattcatctg
     3841 gcccttcctg tgctggaaag taaaaacctg gccttcagca tggtggacct gctgacagag
     3901 gcgaagtcgt ttgctgcaga aggaaccggt tttgctgacc tgggagggga aatcaatgcg
     3961 cagataaaac ggggtgattt actgtatgtg gatgtggcaa aaggctatgg cacaggcctg
     4021 ctggtttccc gtgcgtcgta tgaggcagaa aagagcattc ttcgccatat tctcgaaggt
     4081 aaggaggcgg tcacgccgct gatggagaga gtacctggcg aactcatgga gaaactgaca
     4141 tcaggacagc gcgccgccgc ccgtatgata ctggaaacgt ccgaccgttt cacggtggtg
     4201 cagggctatg ccggtgtggg taagaccaca cagttccggg cggtgatgtc agccgtgaac
     4261 atgctgccgg agagtgaacg tccccgtgtc gtggggctgg ggcccacaca ccgtgcggtc
     4321 ggggagatgc gcagcgccgg cgtggatgca cagacactgg cgtcctttct gcatgacacg
     4381 cagctgcagc agcgcagcgg agaaacgccg gatttcagca acacgctgtt ccttctcgat
     4441 gagagctcta tggtgggcaa taccgacatg gcacgggcat acgccctgat tgcggccggt
     4501 ggcggtcggg ctgtggccag cggtgacacg gaccagctgc aggccatcgc gcccggtcag
     4561 cctttccgtc tccagcagac gcgcagtgct gccgatgtgg tcatcatgaa ggagattgtg
     4621 cgtcagacgc cggaactgcg ggaggcggta tacagcctga ttaaccggga tgtggaaagg
     4681 gcgctgtccg ggcttgagcg tgtgaaaccg tctcaggtgc cacgtctgga gggcgcatgg
     4741 gcaccggagc actccgtgac ggagttcagt cacagccagg aagcgaaact ggcagaagcg
     4801 cagcagaagg cgatgctgaa aggcgaggct tttccggatg tccccatgac actgtatgca
     4861 gccattgtcc gcgactatac cggcaggaca ccggaagcac gggagcagac gctgattgtc
     4921 acgcacctga atgaggaccg gcgcgtactg aacagcatga ttcatgatgc acgggaaaag
     4981 gccggagaac tggggcaggt gcaggtcatg gtgcctgtcc tgaacacagc gaatatacgc
     5041 gacggggagc tgcgtcgtct ctccacctgg gagaataacc cggatgccct tgccctggtg
     5101 gataatgtgt atcaccggat tgccggtatc agcaaggatg acgggctgat aaccctgcag
     5161 gatgcggaag gtaacacgcg gctgatttcg ccccgggagg cggtggctga aggcgtcacg
     5221 ctgtacaccc cggacaccat ccgggtgggg accggtgaca ggatacgctt cacgaagagt
     5281 gaccgggagc gcggttatgt ggccaacagc gtctggacgg tgacagcggt ttccggtgac
     5341 agtgtcacac tttctgacgg tcagcagacc cgtgtgattc gccccggtca ggagcgggca
     5401 gagcaacata ttgacctggc ctatgccatc accgcccacg gtgcacaggg ggcgagtgaa
     5461 acctttgcca tcgcgcttga aggcacggaa ggtaaccgga aactgatggc cggctttgag
     5521 tcagcctacg tggccctgtc gcgtatgaag cagtatgtgc aggtgtacac cgataaccgt
     5581 cagggctgga cggatgccat caacaatgcc gtacagaaag gaactgccca cgatgtgttt
     5641 gagccgaaac cggaccggga ggtcatgaat gcagagcggc tgttcagtac ggcgcgggag
     5701 ctgcgggacg tggcggcagg ccgtgccgtt ctccgtcagg cggggctggc cgggggagac
     5761 agtcctgcac ggtttattgc tccgggacgt aaatatccgc aaccgtatgt ggcactgccg
     5821 gcgtttgacc gtaacggcaa gtccgccggt atctggctga acccgctgac cacggatgac
     5881 ggaaacgggc tgcggggatt cagcggtgaa ggtcgggtaa aaggcagcgg ggatgcgcag
     5941 ttcgtggccc tgcagggcag ccgtaacgga gaaagcctgc tggctgataa tatgcaggat
     6001 ggcgtccgga ttgcccgtga taatcctgac agtggtgtgg tggtaagaat cgccggtgaa
     6061 ggccgtccgt ggaatcccgg tgccatcacc ggtggccgcg tgtgggggga tatcccggac
     6121 aacagcgtcc agccgggagc cggaaatggc gaaccggtca tggcagaggt actggcacag
     6181 cggcaggctg aagaggccat ccgccgtgaa acggaacgtc gcgcagatga aattgtccgt
     6241 aaaatggcag agaacaaacc tgacctgccg gacggcaaaa cagagcaggc tgtcagggaa
     6301 attgccgggc aggagcgtga ccgggctgcc ataactgaac gggaagccgc gctgccggag
     6361 agtgtactgc gtgaaccaca acgggtgcgg gaagcggtcc gggaagttgc ccgggaaaat
     6421 ttgctgcagg agcgtctgca gcagatggag cgggatatgg ttcgtgacct gcagaaagag
     6481 aaaaccccgg gcggagactg atacaggaag acaaacactg atgacaacgg ataacacgaa
     6541 cacgacacgt aacgattcac tggctgcacg gaccgatacc tggttgcagt catttctggt
     6601 ctggtcaccc ggacagcggg atatcatcaa aacggtggca ctggtgctga tggtactgga
     6661 ccacatcaat ctgatattcc agctgaagca ggaatggatg tttctggccg ggcgaggagc
     6721 ctttccgctt tttgccctgg tgtgggggct gaatctgtcc cgtcatgcgc atatccggca
     6781 accggccatt aaccggctgt ggggatgggg aattattgcc cagttcgcat attatctggc
     6841 aggctttccc tggtatgagg ggaatatcct gtttgccttt gcagtggcgg cacaggtgct
     6901 gacgtggtgt gaaacgcgca gtgggtggcg tacagcagcg gccatactcc tgatggccct
     6961 gtgggggcct ttgtccggca ccagttacgg tattgccggg ttgctgatgc tggcagtcag
     7021 caaccggctg taccgggcgg aagacagagc ggaacgtctg gcgctggtcg cctgcctgct
     7081 ggctgttatt ccggcgctta accttgccac cagtgatgca gcggcggtgg ccggtcttgt
     7141 gatgacggtg ctgaccgttg gtctggtgtt gtgtgccgga aaatcattac cccgtttctg
     7201 gcccggagac tttttcccga cgttctatgc ctgtcatctg gctgtgctgg gcgttctggc
     7261 gctgtgacgg gtgtggtatc tttggccgca agaggatgat tcgtcagagg cagaacacag
     7321 catgacagag cagaagcgac cggtactgac actgaagcgg aagacagagg gaacagcgac
     7381 tgtccgcagc cggaaaacca tcatcaatgt caccacgcca ccaaagtgga aggtgaaaaa
     7441 gcagaagctg gccgagaaag cggcccgtga agcagagctg gcggcaaaaa aagcgcaggc
     7501 cagacaggcg ctgtccattt atctgaacct gcccacgctg gatgaggctg tgaataccct
     7561 gaagccctgg tggccgggat tatttgacgg tgacacgccc cggcttctgg cctgcggtat
     7621 ccgggacgtg ttactggaag acgtggcgca gcggaatatc ccgctctcgc ataaaaaact
     7681 gcgcagggcg ctgaaggcca tcacccgttc agaaagctat ctgtgtgcca tgaaagccgg
     7741 tgcctgccgg tatgacacgg aagggtatgt gacggagcat atttctcagg aggaggaagc
     7801 gtatgcggga gcgcgtctgg cgaaaattcg tcgccagaac cggataaagg cagaacttca
     7861 ggccgtgctt gatgagaaat aaaaaaagcc tcccccagag agaggaggca gggaaataag
     7921 gcttaaaagg aaataacttc cgcttccgaa aggaagccag tccactgaat tatttatcac
     7981 aataatactg acaactaaag ggggacaaga aaatgaatgg attcagaaac agttcacgga
     8041 acggccaggt ctggcgttac cagcgtgccg gcgggcgggc cgttattctg gaagtcagtg
     8101 gacgctggat ggaagcggca gaagcatggc gacgggctgc ctgtgttgcg cctaggacag
     8161 actggcagca gtttgcccgg aaaagggctg aacactgcca ccggcgctgc cggggaaggg
     8221 ggtaacgaaa aaagcagcct tcattactcc aggagtcaga aggctgaaag gagtttatga
     8281 gatttgcttc ttaatatccg gtcgggcagt atgaccggac agttctgagt agcacagagt
     8341 cttttccgca ttaagtcgat tttttatatt ttgtgattaa tttcacaaaa taaggtgtta
     8401 ttcaatgtgt gctgcactat tcaggggggc tgaaatacag gtgccattta tctgatatgg
     8461 agaataacat gaggaaatat attccactgg tattatttat cttttcatgg ccggtattgt
     8521 ctgcagatat tcatggacgg gttgttcgtg tactggacgg tgataccatc gaggtcatgg
     8581 actcactgaa ggcggtgcgg atcaggctgg taaatattga tgcaccggaa aagaaacagg
     8641 attatggccg gtggtcaaca gacatgatga aatcactggt tgcaggaaaa acggttaccg
     8701 tcacatattt tcagcgagat cgttatggtc gtattctcgg gcaggtttat gcaccagacg
     8761 gaatgaatat taatcagttt atggtccggg ccggggctgc ctgggtatat gagcagtaca
     8821 acacagatcc cgtgctacct gtccttcaga acgaagccag acaacaaaaa cgtgggctct
     8881 ggtcagatgc tgatccggtt cccccctgga tatggaggca ccgtaaataa agcgatggca
     8941 atcctatcat tcatctgttc tcagcgggaa taaaaatggc aaaaacaaaa caggaatggc
     9001 tgtatcagtt acggcgatgt tcatcagtca atacgctgga aaaaattatt cataaaaaca
     9061 gagacagttt atcaaacagt gaacgtgaat catttaattc agcagcagac caccggctag
     9121 cggaattaat caccggaaag ttatatgaca ggataccaaa agagatatgg aaatatgtaa
     9181 ggtaataatt cagcaataat taattgccag gagcagataa ataatgaagt tgattatttt
     9241 tattctcatt gttctgatta ttgcagcgct acttatccgg attatcctca gaagtgtaaa
     9301 tcagcattct cctttgctga tgcaattgca tgcggcaggt atacggaccg gtgatgcaga
     9361 gcgaatactg tccagcggtg aatactggca acgtcagacg acgctgctga cagaaaggga
     9421 agtcagtttt atgaaaggac tgttcagaat tgtggatatg acgcggtggt atctgtgtcc
     9481 gcaggtacgg gtcgcggata tcgtccagct gaacgggaat atccggccac gatcacgcca
     9541 gtggtggcag ttattcagga tggtgtctca gtggcatgtt gatgtggtta tcgttgagct
     9601 gcgttcgttc agtattgttg cagcagtaga gctggatgat gccagccatt tacgaccgga
     9661 acgcagacgc cgggatattc ttctggaaga ggttctgagg caggctggta ttccgttgct
     9721 cagaagccac gatgccagaa aactgctgca gatgaccgga gaatggctga atacaacagg
     9781 ggctgatcag cagtcaccgg aacatcgtag ctgacgcctt cgcgttgctc agttgtccaa
     9841 ccccggaaac gggaaaaagc aagttttcct cgctcccggc gtttccataa ctgaaaacca
     9901 tactatttca cagtttaaat cacattaaac gacagtaatc cccgttgatt tgtgcgccaa
     9961 cacagatctt cgccacaatt ctcaagtcgc tgatttcaaa aaactgtagt atcctctgcg
    10021 aaacgatccc tgtttgagta ttgaggaggc gagatgtcgc agacagaaaa tgcagtgact
    10081 tcctcattga gtcaaaagcg gtttgtgcgc agaggtaagc ctatgactga ctctgagaaa
    10141 caaatggccg ctgttgcaag aaaacgtctt acacacaaag agataaaagt ttttgtcaaa
    10201 aatcctctga aagatctcat ggttgagtac tgcgagagag aggggataac acaggctcag
    10261 ttcgttgaga aaatcatcaa agatgaactg cagagactgg atatactaaa gtaaagactt
    10321 tactttgtgg cgtagcatgc tagattactg atcgtttaag gaattttatg gctggccacg
    10381 ccataaggtg gcagggaact ggttctgatg tagatgtaca ggagccagaa aagcaaaaac
    10441 cccgataatc ttctttaact ttggcgagtc agaaagatta ccggggccca cttaaaccgt
    10501 atagccaaca atcaagctat gcggggagta tagttatatg cccggaaaag ttcaagactt
    10561 ctttctgtgc tcgctccttc tgcgcattgt aagtgcagga tggtgtgact gatcttcaac
    10621 aaacttatta ccgccaggta aagaacccga atccggtgtt cacaccccgt gaaggtgccc
    10681 ggacgcttcc gttctgcggg aaactgatgg aaaaggcggt gggcttcacc tcccgttttg
    10741 atttcgccat tcatgtggca catgcccgtt cgcttggtct gcgtcggcgc atgccaccgg
    10801 tactacgtcg ccgggccatt gatgcgctgc tgcaggggct gtgttttcac tatgacccac
    10861 tggccaaccg cgtccagtgt tccatcacca cgctggccat tgagtgcgga ctggcgacgg
    10921 agtctgctgc cggaaaactc tccatcaccc gggccacccg ggccctgacg ttccttgcag
    10981 agctgggact gattacctac cagacggaat atgacccgct tatcgggtgc tacattccga
    11041 ccgatatcac gttcacatct gcactgtttg ctgccctcga tgtatcagag gaggcagtgg
    11101 ccgccgcgcg ccgcagtcgt gtggaatggg aaaacagaca gcgcaaaaag caggggctgg
    11161 ataccctggg tatggatgaa ctgatggcga aagcctggcg ttttgttcgt gagcgtttcc
    11221 gcagttacca gacagagctt aagtcccgtg gaatgaagcg tgcccgtgcg cgtcgtgatg
    11281 cggacaggca gcgtcaggat attgtcaccc tggtgaaacg gcaactgacg cgtgaaatct
    11341 cggaagggcg cttcactgcc agtcgtgagg cggtaaaacg tgaagtggag cgtcgtgtga
    11401 aggagcgcat gattctgtca cgtaaccgta attacagccg gctggccaca gcttccccct
    11461 gaaagtgacc tcctcagaat aatccggcac gcgccggagg cattcgcacg cctgaagccc
    11521 gccagtgcac aaaaaatcag cgctacatac aaaaaacaac ctcatcatcc accttctggt
    11581 gcatccggtc ccccctgttt ttgatacaaa acacgcctca cagacgggga attttgctta
    11641 tccacattaa actgcaaggg acttcaccat aaagttacaa ccgttcacgt cataaagcgc
    11701 cagccgtcag ccctacaggg tgcaatgtat cttttaaaca cctgtttata tctcctttaa
    11761 actacttaat tacattcatt taaaaagaaa acctgttcac tgcctgtcct gtggacagac
    11821 agatatgcac ctcccaccac aagcggcggg ccccgaccga agccggttta gttacaacat
    11881 gcaaacataa acccctaaaa aacccaagtc cgacgcagaa ctgaaaccac aaagcccctc
    11941 cctcataact gaaaagcggc cccgccccgg cccgaagggc cggaacagag tcgcttttaa
    12001 ttatgaatgt tgtaactata ttatcatcgc tgtcagtcct ctggctggac gtatcgagta
    12061 cacgctcgta agcggccctc acggccctca cggccctcac ggcccgctaa cgcggagata
    12121 cgccccgact gcgggtaaac ccttgtcggg ctccgaccgc gcacagaagc tctgtcatgg
    12181 ctgaaaacgg gtatggcttg gcagctacca tgttctgtca agaaaaatcg cttgaactcc
    12241 gacgtacagc cccctaagta ctgccagcaa gctgtcagca ctaagtcccc gatacagcgc
    12301 ggcgtagccg cgctaacggg gttaaaaggg cttacctata cttcatcatc gctgaaagcc
    12361 cgcatggcct cgcagggcag agatgggata ggtgaaacct atcaatacgt accggcttac
    12421 gccgggcttc ggcggtgtta ctcctgtacc atatgtaact acatcatgcc gccttacatg
    12481 cgctacgcgc atatgaagac aaatagccca catcaaacgg tttttaggtc ttcagtacgc
    12541 catagcacca gtgcacgagt tttctcatca gcgcaccaat ggcacacatt ttggccttac
    12601 ctctcaggca taatcgctca tacatcgctt tcatcaatgg gttaaatctc agaccacaca
    12661 acgaagccag atacagtttc gcccggattt ccggcggacc aatttttgac agtttcggac
    12721 accctctgac cgaggtgccg gaatattttt caacgggaac gacacctaaa aatgcagccg
    12781 cc
//
LOCUS       DAJTMC010000120        12743 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.120, whole genome shotgun sequence.
ACCESSION   DAJTMC010000120 DAJTMC010000000
VERSION     DAJTMC010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12743
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.120"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            <1..153
                     /locus_tag="OO130_002995"
     CDS             <1..153
                     /locus_tag="OO130_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCR5366316.1"
                     /translation="GMSNVDRLYQTVPQLIKQFVFGGECETPVRKAKHGDSSGVRGAA
                     WLWPQE"
     gene            complement(252..1484)
                     /gene="araJ"
                     /locus_tag="OO130_002996"
     CDS             complement(252..1484)
                     /gene="araJ"
                     /locus_tag="OO130_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HCR5366317.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGKLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMFFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADTPVLAGE
                     TTGVGYSLGVHYLLPDAA"
     gene            complement(1610..4753)
                     /gene="sbcC"
                     /locus_tag="OO130_002997"
     CDS             complement(1610..4753)
                     /gene="sbcC"
                     /locus_tag="OO130_002997"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HCR5366318.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVALLTPEQV
                     QSLTASLQVLTDEEKQLLTAQQQEQQSLNWLTRLDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHTRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NGSLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVKAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHKRQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTTLQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            complement(4750..5952)
                     /gene="sbcD"
                     /locus_tag="OO130_002998"
     CDS             complement(4750..5952)
                     /gene="sbcD"
                     /locus_tag="OO130_002998"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HCR5366319.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIISSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALAESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            6142..6831
                     /gene="phoB"
                     /locus_tag="OO130_002999"
     CDS             6142..6831
                     /gene="phoB"
                     /locus_tag="OO130_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HCR5366320.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            6889..8184
                     /gene="phoR"
                     /locus_tag="OO130_003000"
     CDS             6889..8184
                     /gene="phoR"
                     /locus_tag="OO130_003000"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HCR5366321.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            8591..9910
                     /gene="brnQ"
                     /locus_tag="OO130_003001"
     CDS             8591..9910
                     /gene="brnQ"
                     /locus_tag="OO130_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HCR5366322.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            9986..11359
                     /gene="proY"
                     /locus_tag="OO130_003002"
     CDS             9986..11359
                     /gene="proY"
                     /locus_tag="OO130_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HCR5366323.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            11515..12743
                     /gene="malZ"
                     /locus_tag="OO130_003003"
                     /pseudo
     CDS             11515..12743
                     /gene="malZ"
                     /locus_tag="OO130_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308480.3"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
BASE COUNT         2765 a         3268 c         3463 g         3247 t
ORIGIN      
        1 gggatgagca atgtagaccg tttatatcaa acggttccgc agttgattaa acaatttgtc
       61 ttcggcggcg aatgtgaaac gccggtgcgt aaggcgaagc acggtgattc cagcggcgta
      121 cgcggcgctg cgtggttatg gccacaagag taaaaaacgt aggcaattgg cgcatcatgc
      181 ctgatgcgac gcttgccgcg tcttatcagg cctgcaaaat gtgccagaac cgcgtagggc
      241 ggataaggcg ttcacgccgc atccggcaat aagtaatgaa caccgagact ataacctacc
      301 ccagtggttt cgccagcaag caccggagta tccgccgctt gctggcgctt atagcgacca
      361 tacagcagca acgacgacat cgcagcaaac gaaagcaggg cggcaggcag cgccacgtaa
      421 ttatatgcca gccccagcgt cagcatcata ccaccgcaat atgcgccgac ggcgctaccg
      481 aggttaaacg ctatttgccc acctgcggca cctaataact ctccgccttt ggcgttttgt
      541 agtaacaata tttgtagcgg tgctgaaagg gcaaataatc ccgcgcaaca aataaaagca
      601 aaaataagcg acgttgtttt catgccgccg cagaaaaaga acatcagcag tgccagtaca
      661 attataaagt cagtcactgc tgcaatgcgc agtggtgaat aacgtcctga aatcctgcca
      721 cttagcatat ttcccagcac catccctagc ccaactaaca tcataataaa ggtcatcgcc
      781 gtttccgaaa aaccggaaat aaacatcatg tatggcttta cgtagctgaa ccaggcaaac
      841 acacctgcgt tgccaaacat ggtggcggcg aaaattaacc acggggccgg gctgcgcaaa
      901 aagtgaaatt gttcgcgcag ctttcctttc gcctcgtcgc gaatatccgg cacccaaaaa
      961 tagaccgatg ccatcaccgc aatattaaaa acagcgatca ataaaaaggt gtaacgccag
     1021 ctaaattcct gacttaaata cgttcccagc ggaatgccca gcaaattggc gactgtcatc
     1081 ccggaaacca tccccgccac ggcggcggtg acttttccgg gtttgataat ttttgataac
     1141 acgatcgcgc cgacgccaaa aaatgcgcca tgcggaaagc cggataccag ccgaccaatg
     1201 gcgagcatca ggtaagacga agagagcgtg aacatggcgt tgccaatgac gcacaacgcc
     1261 accagaaaca acaagatatg tttgagtgag tagcggctgg aaaagagtgc gatgattggc
     1321 gcaccgacca ccactcccag tgcataatac gagatcatat gcccggcagc aggaatcgaa
     1381 attcctacgt tatgcgccag ctccgtgagc acgcccataa tgccaaattc ggccatcccc
     1441 aaaccaaacg tgcccagagc caaagataaa atgacttttt tcataccacc atcacaacca
     1501 ccagaacggc taccaatcac cgtagccaat aaagtgatag cttgcaggat aacgaccagc
     1561 aaggccaaga gataattcgc ccctctgtat tcattatcct gctgaatagt tatttcactg
     1621 caaacgtact ttccagtttg ctgtagccca ggccgttgat ctttttcact ttgatctgca
     1681 ccggaatacg ctctttcatc gcttctacgt ggctaatcac accgatggtt ttgccactgg
     1741 cgttcagggc atccagcgca tcaagggcgg tatccagcgt ttcgctatcc agcgtgccaa
     1801 aaccttcatc aaggaacagc gagtcaatac gtgttttatg gctgaccaga tccgaaagcg
     1861 ccagcgccag cgccagacta acgaggaaac tttcgccgcc ggaaagggta cgggtatcgc
     1921 gtaccgcatc tgcctgccag gtatcaacaa cctcgacttc cagcgcctcg ctggctttgc
     1981 gctgtaacag atagcgcccg tgcagccggg taagttgctg attagcgaga tggactaaat
     2041 tatccagcgt cagcccctgg gcaaacttgc ggaatttatc gccctctttg gaacctatta
     2101 gcgaattcag atatccccag tcctcaacct gctgcgtcat ttgagcaatt tgctgcatta
     2161 aggtttgttg ttgctgacgg ttatctgcat cctgcttcag ctgctggcga atctcgcctt
     2221 gactcgtggt gttttcacgc aacttttggt gagtttgcgc taactcttgc tgaatctgct
     2281 ccaccgtcac agtgagagcc aacccgtcag gtcggtgttg ttgatgctgt gccagcgttt
     2341 ctgctgtctg agtgaccagc gtttgcgcct gacggcgctg gttttccaga ttctgcttga
     2401 gctgttccag ctgcgttagt gtttgttcat ccattagcgc cgcaaggaac gcctgctgat
     2461 cgtcaaagac gctggcctgt agcgcggtgt caaactgcgc ctgggctttt tgcagacttt
     2521 gcgccgccag aacatcctgt tgctgtaacg tctgctgctg gctgtgtaat gcgagacatt
     2581 gttcatgaac ctgccgccag ttatccagcg ccacagtttc ttcgctgtgc gggagatcat
     2641 cactttgcgg caacgtttcc agaatcggcg tcagctgctg aatacggttt tgcagcgtgg
     2701 ttaattcgtt ctggcgttgc tgccagctct gcgcttcttg ctgacgtgtc gccaaccagc
     2761 tctcttcttc atcttcctgt ggcaatgtca gtgcataacc cgccaatgcc gttaagagct
     2821 gctgctggcg ttgttcaatt tgctgttgat actggataat ttgctgatta tgcgcggcaa
     2881 tctgcccttg taattcatgt cgttggctga gtaaccgcag ctggcgtttg tgctcatctt
     2941 gtgcatccag ccacggttga atatcgtcct gtggctgcaa ggtgatattg aggctggccg
     3001 tgacggcttg ccattgttga gtaagtgctt gctcatcttg tcggaggctt tgcgcttcgt
     3061 tttcatcgcg ctgaagctgc ttcgttaatg catccagttg cccgcgcagc gccgcacctt
     3121 cttcgccgag ctttttaact tcgttttcca gcgccagtaa tcgagactga ttaacaccag
     3181 gctccagcgc ctgatacgcc ttgaccgccg ggtggctggt ggaaccacaa agtggacaag
     3241 gctgacccgc ctgtaactgt gcacgctggg cttccagcgt tttgatgcgc gcttcctgct
     3301 cgcaaatggt tttcacatcg gcaagttgct gcgtcttttc tttataacgc tggcgcattt
     3361 cgttaagtgc gacattacgt tgcgtctgtt ccagagatcc attctggata gtgaccatta
     3421 actgcgccag acgtttttgt tggggaacaa tctgcccatg cagcgcgacc aggcgctgac
     3481 gcagtgggcg ttgctcagca tgttgcgcca gggcggtagc aacttcatcg gcggttaacg
     3541 tcaacgtgat cgccgcaagc gcattaagtt tttgctcagc atgggttaac tgttgctgcc
     3601 attgccgcag atgctcgcga tcgctggttt gttgggagaa ctgcgcacgc caacccgcca
     3661 gttcgttgtt ccactgacgg aagcggtcgt gttcctgtaa ccaggtattc aggctttgtt
     3721 gctgctgctg taattctgct gactgcttcg ccgcgtggtg gcgaatgctc gcgcgaagcg
     3781 ccattgtgct ctgtaagcga gtatttactt cttcaatctg ctggcgagta tgcgccagcg
     3841 ccgcgctgtg ttctgcgatg cgttcccagt gcggacgaag atttcgtgcc ggttgtgcca
     3901 gactgagcgc cgccagttga ggttgcgctt tttcttcttc ggctaacgcc tgttgcaagg
     3961 cctgctgacg gcggctggct tcttgctgca attcgtccag acgcgttaac cagtttagtg
     4021 attgttgttc ttgctgctgc gcggtaagta actgtttttc ttcgtcagta agtacctgca
     4081 aactcgctgt cagcgattgc acttgttccg gcgtgagcaa cgcgacgccg ctggcctgcg
     4141 cttgcagctt ctccagctct gtgcgggccg atttgtgctg ctcaaaaacc atcgccgaga
     4201 tttgcccgta gatttcagtg ccggttaact cctcgagcaa ttccgcgcgt tctttgggtt
     4261 tggcattcag gaaggcagca aattgcccct gcgaaagcag catcgaacgg gtgaagcgcc
     4321 cgtaatccag cccggttaac gtcgctgtca gttccagctt atctttcact ttgtcggcga
     4381 gaattttgcc gtcggcgcag cgcgccagct ctacgcgtgg cacctgcaaa ttaccgtcgg
     4441 gttggttacg cgcccgattc tggctccaga atgcacggta cgcttcacct ttcacttcaa
     4501 actccacctc cgccagacat tcggcggtat cgcgggtcat gagatcattt tgcgattgtg
     4561 aaacgttaga gagacgcgga gtttcgtgat acagcgccag acaaatggcg tccagcaggg
     4621 tggttttccc cgcaccggtt gggccggtaa tggcaaacag cccgttgctg gcgaacggct
     4681 cgcgggtgaa atcaatcttc cattcgcctt ttaatgagtt caggtttttc aggcgcaggc
     4741 tgagaatttt catgcttcgt gttctccggc gagggtatgc aacgtcgtgg tgaaaagatg
     4801 ctgcagacgt tgctgctgcg attcatccag ttcttccagt gccagacggc gattgaacac
     4861 ctcttcgacg ctgagttcgc tgagggtttc acgctgttgg ctggctaaca cacgctcgcg
     4921 ctgttcacga ctccgacgta ccagcaatac ttcgacaggc aatgattcgg ctaatgcctg
     4981 gattttgcgc tgaatatcat gcagatactc atcagtagtg atttcgatat ccagccagac
     5041 aggtggctcc tgcgatacat cgcgccactg ttccagctgt gcggtaatcg acgccagatc
     5101 gcctttcagc accgccatgg gttgagttac cggtacgttc aggttttcca cgctctctaa
     5161 tttgccgttt gaaaatgtca ccagatggac atatttactc ttaccgcatt catcaaaact
     5221 cagtggaatg ggggaaccgc aatagcgaac atgttccatg ccgccaataa tctgtgcgcg
     5281 gtgaatatgc ccgagcgcga tgtagtcggc tggtggaaag ttttgtgccg gaaacgcgtc
     5341 cagcgtgcca atataaatgt cacgcacggc gtcactttta ctggcaccca cggtcgttaa
     5401 atgtcccgtg gcgatgatgg gcagaggctg atcgccgcgc agtttgcagg catcggcata
     5461 gtgttgttgg taataatcgg taatcgctgc cagtaaatgc tgctgttttt caataccgtt
     5521 aagccccgcc tggctggaaa taatgtcacg cgggcgtaaa aacggaatgg ggcacagcac
     5581 tgcgcctggc gtcccgtcgc gacgaggcaa gatttgcggc gcatgtccgg cgctggcgac
     5641 cacggttgta ttgaggaacg ccatgatatc gcgcgattca ttcagcgtgg cgaccgagtc
     5701 atggtttcct gccagtacca ccagatgaca gccagtttgc tgtaaattga caacaaaacg
     5761 gttgtataac gtgcgggcgt aactgggcgg cgagccggta tcgaaaacat caccagcaac
     5821 aataatcgca tccacctgat gggtttgtgc tgtctccagc agccagtcaa gaaaagcctg
     5881 atgttcagct tcgcggcttt tactgtagaa gttctggccg agatgccagt ctgaggtgtg
     5941 aaggatgcgc ataacggttc cctggcgaaa aagcatggtc gcgattatac ccaaacagat
     6001 gtgccatttg cttttttctg cgccacggaa atcaataacc tgaagatatg tgcgacgagc
     6061 ttttcataaa tctgtcataa atctgacgca taatgacgtc gcattaatga tcgcaaccta
     6121 tttattacaa cagggcaaat catggcgaga cgtattctgg tcgtagaaga tgaagctcca
     6181 attcgcgaaa tggtctgctt cgtgctcgaa caaaatggct ttcagccggt cgaagcggaa
     6241 gattatgaca gtgctgtgaa tcaactgaat gaaccctggc cggatttaat tctccttgac
     6301 tggatgttac ctggcggctc cggtatccag ttcatcaaac acctcaagcg cgagtcgatg
     6361 acccgggata ttccagtggt gatgttgacc gccagagggg aagaagaaga tcgcgtgcgc
     6421 ggccttgaaa ccggcgcgga tgactatatc accaagccgt tttcgccgaa ggagctggtg
     6481 gcgcgaatca aagcggtaat gcgccgtatt tcgccaatgg cggtggaaga ggtgattgag
     6541 atgcagggat taagtctcga cccgacatct caccgagtga tggcgggcga agagccgctg
     6601 gagatggggc cgacagaatt taaactgctg cactttttta tgacgcatcc tgagcgcgtg
     6661 tacagccgcg agcagctgtt aaaccacgtc tggggaacta acgtgtatgt ggaagaccgc
     6721 acggtcgatg tccacattcg tcgcctgcgt aaagcactgg agcccggcgg gcatgaccgc
     6781 atggtgcaga ccgtgcgcgg tacaggatat cgtttttcaa cccgctttta acgccttgct
     6841 catcggacgc agagcagggc ttatgatttc ttaactggag tatcttacgt gctggaacgg
     6901 ctgtcgtgga aaaggctggt gctggagctg ctactttgtt gcctcccggc tttcatcctg
     6961 ggtgcatttt ttggttacct gccctggttt ttgctggcat cggtaacagg actgcttatc
     7021 tggcatttct ggaatttatt gcgcctttca tggtggttgt gggtggatcg cagtatgacc
     7081 ccgccaccgg ggcgtggtag ctgggaaccg ctactatacg gcttacacca gatgcagctg
     7141 cgaaataaaa aacgccgccg tgaactgggc aatctgatta aacgctttcg tagcggcgcg
     7201 gagtcgctgc ccgacgcggt ggtgctgacc acggaagagg gcggtatttt ctggtgtaac
     7261 ggtctggcgc aacaaattct tggtttgcgc tggccggaag ataacgggca gaacatcctt
     7321 aacctactgc gttacccgga gtttacgcaa tatctgaaaa cgcgtgattt ttctcgcccg
     7381 ctcaatctgg tgctcaacac cgggcggcat ctggaaattc gcgtcatgcc ttatacccac
     7441 aaacagttgc tgatggtggc gcgtgatgtc acgcaaatgc atcaactgga aggggcgcgg
     7501 cgtaactttt ttgccaacgt gagccatgag ttacgtacgc cattgaccgt gttacagggt
     7561 tacctggaga tgatggatga gcagccgctg gaaggcgcgg tacgcgaaaa agcgttgcac
     7621 accatgcgcg agcagaccca gcggatggaa gggctggtga agcagttgct aacgctgtcg
     7681 aaaattgaag ccgcgccgac gcaattgctc aatgaaaagg ttgatgtgcc gatgatgctg
     7741 cgcgttgttg agcgcgaggc tcagactctg agtcagaaaa aacagacatt tacctttgag
     7801 atagataacg gcctcaaagt gtctggcaac gaagatcagc tacgcagtgc gatttcgaac
     7861 ctggtctata acgccgtgaa tcatactcca gaaggcacgc atatcaccgt acgctggcag
     7921 cgagtgccgc acggtgccga atttagcgtt gaagataacg gaccgggcat tgcaccggag
     7981 catattccgc gcctgaccga gcgtttttat cgcgttgata aagcgcgttc ccggcaaacc
     8041 ggcggtagcg gattagggtt agcgatcgtg aaacatgctg tgaatcatca cgaaagtcgc
     8101 ctgaatattg agagtacagt aggaaaagga acacgtttca gttttgttat cccggaacgt
     8161 ttaattgcca aaaacagcga ttaatccgcc tttgtcatct tttattgcca taagccagtc
     8221 gatgctggct tattttcttt gcagtcaaaa tacgggcgtt agattttaca acggttggtg
     8281 attttttgtt cgcatgatta gccatgtctt tttcacggaa atagtgtttt atactggttg
     8341 gtgatttctt atcgctatat acctctggtt tttagatccc tccttgcttt aaaacgttat
     8401 aagcgtttaa attgcgcttc aggtgctgtc atactgactg cattaacgcg gtaaatcgaa
     8461 aaactattct tcgccgcgcc aggttgggag tatttcccgc taaaattgtt taaatatacc
     8521 gctgtatcat ccccagggat tggcacaaaa atttaacgtt acaacaccac atccacaggc
     8581 agtatgattt atgacccatc aattaagatc gcgcgatatc atcgctctgg gctttatgac
     8641 atttgcgttg ttcgtcggcg caggtaacat tattttccct ccaatggtcg gcttgcaggc
     8701 aggcgaacac gtctggactg cggcattcgg cttcctcatt actgccgttg gcctgccggt
     8761 attaacggta gtggcgctgg caaaagttgg cggcggtgtt gacagcctca gcacgccaat
     8821 cggtaaagtc gctggcgtgc tgctggcaac ggtttgttac ttggcggtgg ggccgctttt
     8881 cgctacgccg cgtacagcta ccgtttcctt tgaagtgggg attgcgccgc tgacgggtga
     8941 ttccgcgctg ccgctgttta tctacagcct ggtctatttc gctatcgtta ttctggtttc
     9001 gctctatccg ggcaagctgc tggataccgt gggcaacttc cttgcgccgc tgaaaattat
     9061 cgcgctggtc atcctgtctg ttgccgctat tgtctggccg gcgggttcta tcagcacggc
     9121 gactgaggct tatcaaaacg ctgcgttttc taacggcttc gttaacggct atctgaccat
     9181 ggatacgctg ggcgcaatgg tgtttggtat cgttattgtt aacgcggcgc gttctcgtgg
     9241 cgttaccgaa gcgcgtctgc tgacccgtta taccgtctgg gctggcctga tggcgggtgt
     9301 tggtctgact ctgctgtacc tggcgctgtt ccgtctgggt tcagacagcg cgtcgctggt
     9361 cgatcagtct gcaaacggcg ctgctattct gcatgcttac gttcagcaca catttggcgg
     9421 cggcggtagc ttcctgctgg cggcgttaat cttcatcgcc tgcctggtaa cggcagttgg
     9481 cctgacctgt gcttgtgcag aattctttgc ccagtacgta ccgctctctt atcgtacgct
     9541 ggtgtttatc ctcggcggct tctcgatggt ggtgtctaac ctcggcttaa gccagctgat
     9601 ccagatctcc gtaccggtgc tgaccgctat ttatccgccg tgtatcgcac tggttgtatt
     9661 aagttttaca cgctcatggt ggcataattc gtcccgcgtg attgccccgc cgatgtttat
     9721 cagcctgctt tttggtattc tcgacgggat caaagcatct gcattcagcg atatcttacc
     9781 gtcctgggcg cagcgtttac cgctggccga acaaggcctg gcgtggttaa tgccaacagt
     9841 ggtgatggtg gttctggcca ttatctggga tcgcgcggca ggtcgtcagg tgacctccag
     9901 cgctcactaa atcactgaac atttgtttta accacggggc tgcgatgccc cgtggttttt
     9961 tattgtgttg atgggttagg aattgatgga aagtaagaac aagctaaagc gtgggctaag
    10021 tacccgccac atacgcttta tggcactggg ttcagcaatt ggcaccgggc tgttttacgg
    10081 ttcggcagac gccatcaaaa tggccggtcc gagcgtgttg ttggcctata ttatcggtgg
    10141 tatcgcggcg tatatcatta tgcgtgcgct gggggaaatg tcggtacata acccggccgc
    10201 cagctctttc tcgcgttatg cgcaggaaaa cctcggcccg ctggcaggtt acattaccgg
    10261 ctggacctac tgctttgaaa tcctaattgt cgccatcgcc gatgtgaccg cttttggtat
    10321 ctatatgggt gtctggttcc cgacggtgcc gcactggatt tgggtactga gcgtggtgct
    10381 gatcatttgc gccgtaaacc tgatgagcgt gaaggtattc ggtgagctgg aattctggtt
    10441 ctcgttcttt aaagtcgcca ccatcatcat catgattgtc gccggtttcg gcatcatcat
    10501 ctgggggatt ggcaacggcg ggcaaccgac cggtattcat aacctgtgga gcaacggcgg
    10561 cttcttcagt aacggctggc ttggcatggt aatgtcgttg caaatggtga tgtttgctta
    10621 cggtgggatc gaaattatcg ggattaccgc cggtgaagcg aaagatcctg agaaatcgat
    10681 tccgcgtgcg attaactccg tgccgatgcg tattctggtg ttctacgtcg gtacgctgtt
    10741 cgtcattatg tctatctacc cgtggaatca ggttggcact gccggtagcc cgttcgtgct
    10801 gacgttccag catatgggca ttacctttgc cgccagcatt cttaactttg ttgtgctgac
    10861 cgcttcgctg tcggcaatta acagtgacgt atttggcgta ggccgtatgc tccacggtat
    10921 ggcagagcag ggcagcgcac cgaaaatttt cagcaaaacg tcgcgtcgcg gtattccgtg
    10981 ggttacggtg ctggtgatga ctaccgcgct gctgtttgcg gtgtatctga actacatcat
    11041 gccggaaaac gtcttcctgg tgatcgcttc gctggcaacc ttcgccacgg tatgggtgtg
    11101 gattatgatc ctgctgtcgc aaatcgcctt ccgtcgccgt ttgccgccag aagaagttaa
    11161 ggcgctgaaa tttaaagtgc cgggtggggt agcaacgacc atcggcgggc tgattttcct
    11221 gctctttatt atcgggttga ttggttatca cccggatacg cgtatctcgc tgtatgtcgg
    11281 tttcgcgtgg attgttgtgc tgttgattgg ctggatgttt aagcgtcgcc acgatcgtca
    11341 gctggctgaa aaccagtaat ccttgcgttc tgtaagccgg ttcaggagga aatcctgatc
    11401 cggctattcc gaaagttatc cgcccccgtc ctcctccccc aaatatcctt cagatgatga
    11461 gtgatcctgc attaggctat ggcaaggtga tcagattttc atcacagggg aattatgatg
    11521 ttaaatgcat ggcacctgcc ggtgccccca tttgttaaac aaagcaaaga tcaactgctc
    11581 attacactgt ggctgacggg cgaagaccca ccgcagcgca ttatgctgcg tacagaacac
    11641 gataacgaag aaatgtcagt accaatgcat aagcagcgca gtcagccgca gccaggcgtc
    11701 accgcagggc ttcagccgaa tgccgccggc acgactggag cagtttgccg tcgatgtacc
    11761 ggatatcggc ccacaatggg ctgcggatca gattttttat cagatcttcc ctgatcgttt
    11821 tgcgcgtagt cttcctcgtg aagctgaaca ggatcatgtc tattaccatc atgcagccgg
    11881 acaagagatc atcttgcgtg actgggatga accggtcacg gcgcaggcgg gcggatcaac
    11941 gttctatggc ggcgatctgg acgggataag cgaaaaactg ccgtatctga aaaagcttgg
    12001 cgtgacagcg ctgtatctca atccggtgtt taaagctccc agcgtacatc aatacgatac
    12061 cgaggattat cgccatgtcg atccgcagtt tggcggtgat ggggcgttgc tgcgtttgcg
    12121 acacaatacg cagcagctgg gaatgcggct ggtgctggac ggcgtgttta accacagtgg
    12181 cgattcccat gcctggtttg acaggcacaa tcgtggcacg gggggagctt gttacaaccc
    12241 tgaatcgccc tggcgcgact ggtactcgtt tagtgatgat ggcacggcgc tcgactggct
    12301 tggctatgcc agcttgccga agctggatta tcagtcggaa agtctggtga atgaaattta
    12361 tcgcggggaa gacagtattg tccgccattg gctgacagcg ccgtggagta tggacggctg
    12421 gcggctggat gtggtgcata tgctggggga ggcgggtggg gcgcgcaata atatgcagca
    12481 cgttgctggg atcaccgaag cggcgaaaga aacccagccg gaagcgtata ttgtcggcga
    12541 acattttggc gatgcacggc aatggttaca ggccgatgtg gaagatgccg ccatgaacta
    12601 tcgtggcttc acattcccgt tgtggggatt tcttgccaat accgatatct cttacgatcc
    12661 gcagcaaatt gatgcccaaa cctgtatggc ctggatggat aattaccgcg cagggctttc
    12721 tcatcaacaa caattacgta tgt
//
LOCUS       DAJTMC010000121        12658 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.121, whole genome shotgun sequence.
ACCESSION   DAJTMC010000121 DAJTMC010000000
VERSION     DAJTMC010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12658
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.121"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(4..324)
                     /gene="chbB"
                     /locus_tag="OO130_003004"
     CDS             complement(4..324)
                     /gene="chbB"
                     /locus_tag="OO130_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HCR5366324.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(622..960)
                     /gene="osmE"
                     /locus_tag="OO130_003005"
     CDS             complement(622..960)
                     /gene="osmE"
                     /locus_tag="OO130_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HCR5366325.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            1162..1989
                     /gene="nadE"
                     /locus_tag="OO130_003006"
     CDS             1162..1989
                     /gene="nadE"
                     /locus_tag="OO130_003006"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HCR5366326.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLAALACPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPQQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            2219..3106
                     /gene="cho"
                     /locus_tag="OO130_003007"
     CDS             2219..3106
                     /gene="cho"
                     /locus_tag="OO130_003007"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HCR5366327.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQTIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEDATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(3066..3641)
                     /gene="ves"
                     /locus_tag="OO130_003008"
     CDS             complement(3066..3641)
                     /gene="ves"
                     /locus_tag="OO130_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HCR5366328.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFAADQVVKAKLTEGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(3844..4314)
                     /gene="spy"
                     /locus_tag="OO130_003009"
     CDS             complement(3844..4314)
                     /gene="spy"
                     /locus_tag="OO130_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HCR5366329.1"
                     /translation="MRKLTALFVASTLALGAANLAHAAAPADAKPMMHHKGKFGPHQD
                     MMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIVSDTFDKAKAEAQIAK
                     MEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATAE"
     gene            complement(4644..5612)
                     /gene="astE"
                     /locus_tag="OO130_003010"
     CDS             complement(4644..5612)
                     /gene="astE"
                     /locus_tag="OO130_003010"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HCR5366330.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(5605..6948)
                     /gene="astB"
                     /locus_tag="OO130_003011"
     CDS             complement(5605..6948)
                     /gene="astB"
                     /locus_tag="OO130_003011"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HCR5366331.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRLAAKQ
                     GLLKMKTLADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAPVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            complement(6945..8423)
                     /gene="astD"
                     /locus_tag="OO130_003012"
     CDS             complement(6945..8423)
                     /gene="astD"
                     /locus_tag="OO130_003012"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HCR5366332.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVEQACR
                     AARAAFPRWARLSLAERQVVVERFAGLLESNKAELTAIIARETGKLRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGRANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGAQGDAFLARLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPRLLQLETSLLTPGIIEMTGVAGVPDEEVFGPLLRVWRYDSFEEA
                     ILMANNTRFGLSCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(8420..9454)
                     /gene="astA"
                     /locus_tag="OO130_003013"
     CDS             complement(8420..9454)
                     /gene="astA"
                     /locus_tag="OO130_003013"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HCR5366333.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWHNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAVEKTA"
     gene            complement(9451..10671)
                     /gene="astC"
                     /locus_tag="OO130_003014"
     CDS             complement(9451..10671)
                     /gene="astC"
                     /locus_tag="OO130_003014"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HCR5366334.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIMAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPPDIRHAAYNDINSASALIDDATCAVIVEPIQGEGGVVPASNAFLQGLRELCDRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGDFPVGALLTTEECASVMTVG
                     THGTTYGGNPLASAVAGKVLELINTPEMLNGVKQRHDWFVERLNTINHRYGLFSEVRG
                     LGLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAAACEHFVSRGSS"
     gene            11117..11923
                     /gene="xthA"
                     /locus_tag="OO130_003015"
     CDS             11117..11923
                     /gene="xthA"
                     /locus_tag="OO130_003015"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HCR5366335.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            12090..>12658
                     /locus_tag="OO130_003016"
     CDS             12090..>12658
                     /locus_tag="OO130_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCR5366336.1"
                     /translation="MNAERKFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFLLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITL"
BASE COUNT         3076 a         3426 c         3098 g         3058 t
ORIGIN      
        1 aaattaattt gctgcggctt ttttaatcgc tgcaaccgca gccttaagca cgcctaaacc
       61 atcgactttg ccataaagca gcgagtcaat tacttcaacc ggtttgttgg gtaacaaacg
      121 ctggatttcg ggcaacatat aagcaatctg cggccctaat aacacgacat cggcattctg
      181 acctttttca ccagccagtg tttccggaaa tgcttcaata atgaccggaa cttcatattt
      241 ttctgcctgt gcgcgcattt ttgataccag taaagaggta gacatgcccg cagaacaaaa
      301 cagataaatg tgtttctttt ccataaaact gccctcatcg acgattatct gtcagccaga
      361 cactccgcaa gccttaacct gcttccatgc tctgggtaac ttgcgaaacc aacatgactt
      421 tttttgtggc tgaaaggagt atacggtata gacctgagca atagtatttc tttgacctta
      481 taaattgtct ctccttgacc tgcggttatg accctcttca catttcgggc aaataattcg
      541 cgaagataat taagaactca ggccaaaaaa accggcgcaa tggccggttt ccgttgttac
      601 tcaagctttc aaacgaagtg attacttcgc agcctgtgga tcagtgtcgt attcagcaca
      661 ggtctgataa ccggagttga tgacatgtcc ggtatcatct aacgcgacaa agtaggtttc
      721 tgctttacca tcacgttgac ccaggatgta ggtctggcaa gtaccacgag catggatcat
      781 gctcacttca gacgaaggtt tacccgcaat ttgtgcaacc tgcgcccggc tcatgccttt
      841 tttgacgtct ttcaccacag gctgtacaaa ctggtctttg gtacgatcat aagccgtaca
      901 acccgccagc atggttaata ccgccgctgc actcagaatt cctgccatat tcttgttcat
      961 attccgtcct cttgtttatc agcgtgttag ataagcctgg aatacattgg gcgctttttc
     1021 aagcccgtga acgaaacggc tccgctttca gaggattcct gtatgacgtt ttatccacca
     1081 ttcaacccgc tgtcgcttgt cgtttcagta gcgacgggtt agctttaagg aagttttgtc
     1141 ttttctgtct ggaggggttc aatgacattg caacaacaaa taataaaggc gctgggcgca
     1201 aaaccgcaga ttaatgctga agaggaaatt cgtcgtagtg tcgattttct gaaaagctac
     1261 ctgcaaactt atccgttcat taaatcactg gtgctcggga tcagcggcgg tcaggactcc
     1321 acgcttgccg gtaagctgtg ccagatggcg attaatgagc tgcgccagga gaccggcaac
     1381 gaatcactgc aatttattgc cgtacgcctg ccctatggtg ttcaggccga cgaacaagat
     1441 tgccaggatg ccattgcctt tattcaaccg gatcgcgtat taaccgttaa tatcaagggc
     1501 gcggtattgg ccagcgaaca ggcgctgcgc gaagcgggca ttgaactgag cgattttgtc
     1561 cgtggcaatg aaaaagcgcg tgagcggatg aaagcacaat atagcattgc gggtatgacc
     1621 agcggtgtcg tggtgggcac cgatcatgca gcagaagcta ttaccggatt cttcactaaa
     1681 tatggtgacg gcggtacgga cattaatccg ctgtatcgtc tcaacaaacg tcagggtaaa
     1741 cagttactgg cggcattagc ttgcccggaa cacctttata agaaagcgcc aacggccgat
     1801 ctggaagatg atcgcccttc tctgccagat gaagtggcac tcggcgtgac ctatgacaat
     1861 atcgacgact atctggaagg gaaaaacgta cctcaacagg tcgccagaac aatagagaac
     1921 tggtatctga aaaccgaaca taaacgccgt ccgccaatta ccgttttcga tgatttctgg
     1981 aaaaagtaat aatttgcaca tattggattg tgcgaaaaag agtaatttgt tcacgccgga
     2041 tgcggcgtga acgccttatt cgacctataa aactatgcaa attcaataca ttgcaggagt
     2101 cgaataggcc tgacaggcgt agcacgtcag acggtgtaac ctttgtcatc gacccgcctc
     2161 ttttttaatc gcttcccgcc tgttacactg gatagataac cagcattcgg agtcaacagt
     2221 ggtacggcgt ttaacttctc cgcggctcga atttgaagct gcggcaattt atgaatatcc
     2281 cgaacattta cgttcattcc ttaatgactt acccacccga ccaggggtgt atctgtttca
     2341 tggtgaaagt gacaccatgc cgctctatat cggcaaaagc gttaacatcc gcagccgcgt
     2401 cctttctcat ttacgtaccc cggatgaagc cgccatgcta cggcaatccc gacggatcag
     2461 ctggatatgt accgccggtg aaatcggcgc tctgctcctt gaagcgcgat taatcaaaga
     2521 acaacagccg ctgtttaata aacggttgcg ccgcaatcgt cagctctgtg ccctgcaatt
     2581 aaatgaaaag cgcgtcgatg tggtgtatgc caaagaggtg gatttttcac gagcccccaa
     2641 cctgtttggc ctgtttgcca ataggcgcgc agctttgcaa gcattgcaga ccatcgctga
     2701 tgaacaaaaa ctttgttatg gcctgctggg actggaaccg ttaagtcgcg gtcgtgcatg
     2761 ttttcgttca gcgctaaaac gttgcgccgg agcatgctgc ggtaaagaga gccatgagga
     2821 acatgcgcta cgcttgcgcc agtctctgga gcgtttgcgg gtggtgtgtt ggccttggca
     2881 aggggcggtg gcgctgaaag aacagcaccc ggaaatgact caatatcata ttattcaaaa
     2941 ctggctgtgg ctgggggcgg ttaattcgct ggaagacgcg acaacgttaa ttcggacacc
     3001 cgccgggttt gatcacgacg gttataaaat tctttgtaag ccgctgcttt ccggtaacta
     3061 tgaaattact gaacttgatc cggcgaatga ccagcgagcc agttgatttc actgaacaac
     3121 agtttacctt gcggttgtaa aagccgcagg gtgtgcctgc catcgaacca gcacgcgccc
     3181 tggtcggtag tgagcaattt atcgcccagt tgccacgccc cgttaatcac aaacaccacc
     3241 ccgccgcgtg agccaaaggt agtaaacgta cgttcggcaa ttctgacttt cgccttacag
     3301 acatccaggc gtgtcatgat gttgaaatcc atcgacatct gcccttccgt cagtttcgct
     3361 tttaccacct ggtccgctgc aaaggcaaaa ggctgtaacg gttttaaggt atggttaaag
     3421 cggtctgcgc tttcaaggag catctcgccg ccttccagca acgtcactat cctttccatg
     3481 ccgggaaaca aagaaaactc cccattcgcc gcgatggatg caatgctggc acgccagtaa
     3541 aaatcacgtt ttgccggtgg gaaagtgcaa atttcgcgcg tttcaccggc agcatttcgc
     3601 cacagattca ccgacatttt acgcatatca aagtattcca tactcgctcc cggcccgttc
     3661 ttttcattgt tatcgttcag gtaaccgtgg atggttatcg gcaactcggg tgatttacgt
     3721 tagtggtgat caggcttttt ttacaactgt ttgagaagag aaaagaaaac cgccgatcct
     3781 gtccaccgca ttactgcaag gtagtggaca agaccggcgg tcttaagttt tttggctgaa
     3841 agattattca gcagttgcag gcattttacc ttttgccgct ggacgttctg tcagacgctt
     3901 ctcaaaatta gcattaaatt gctttttctg ttccggcgtc aggatgttgt aaattttgtt
     3961 ctgggtttcc atgtgcgcca gcatgttagc tttgcgctgt tcttccattt ttgcgatctg
     4021 cgcttcagct tttgctttat cgaaggtatc gctgacaatg atgtcatgca ttgcgcggcg
     4081 ttcttccagc ggcggacgtt tcatctggtc acgctggcct ttcatgattt cgcggatctg
     4141 ctgtttctgc gcgtcggtca ggttcaggtc tttgaacatc atgtcctgat gcggaccgaa
     4201 cttgcctttg tggtgcatca tcggcttcgc gtcagccggt gctgcggcat gggccaggtt
     4261 agccgcgcca agagccaggg tagaggcaac aaacagtgca gttaatttac gcatattcta
     4321 tatccttcct ttcagttatt tattacggct ttcttaagta gcgtgccgtg ttgacaagat
     4381 taactttact gactttagcg tcaattaatc agagcaacgg taaaacaatg aaagtgtaaa
     4441 aaacactttt gcgccaatta tggagaaaaa aagaaaattt gatggagagt gatgagagaa
     4501 tattacaaca caatgatttt gcagagatta tgaagaacta tactggatga ctggtgataa
     4561 ataaagcaaa taaccaggat taatctgtat taatttataa gaaagcaact taatacccgc
     4621 agaatgattt ctgcgggtaa gtattagctt attttttcga gcattaatcc cgcgcgtaat
     4681 cccaacgcta ccaacggatt agggaataac acatactcta catcatgggt tacggtaaaa
     4741 cgttcctctc cgtcctgcgc cagcaatgtt cctttctcaa acggcataaa attcagcgtg
     4801 tcacttgcca tatgcatttc gaaggacggc gagtgacgag taatttgcga aaccacccga
     4861 taacggagcg gcggtgttct cacgataccg acactctcac cagatagcag cgcagcaatt
     4921 gcgctggcag ttactgcaaa ctggcgaaga tcgttttgcc caaagggcaa cgctttgcca
     4981 agttccagcg tacaggccag cgcgccaaaa tgtctggcgc tgaaatgggt aaacgtacca
     5041 ccaggttcct gatggaacac cagcgcctcc agccccgccg cacccagcca cgtcagaaat
     5101 ttctcgtccc agggaatgtc gcgttgcggt aatacaccga actgcggatg caaggagcca
     5161 cgaattgcgg tatgtagatc aaggtgccag cgcacagatt ctttgccctg gtcataaaaa
     5221 tcttccaggc actgttccag ttcgcgcgcc cgacaggttt ctccgctttc agcaaatagc
     5281 tgccaacgac cgccaaacat tcgattcata tcgctatggc aataacgttt cccttgcttc
     5341 agcgcaggag gattcccgag gatcaccagc aaccgccaac gtaacgggat ctcgccgtga
     5401 gatatcgcgc caagcaacgc gtccagcatt tccacaggtg ccgtctcatt accgtggatc
     5461 cccgcagaaa tcaccagcgc gccttgcggt ggcgttaatg gcgtcagttc cagcacacca
     5521 tcgcccagcc agcgccagcg aacgccgttg atttctcgct cggtgataac cggtttttta
     5581 cccgttaagg tcagagcaag aaaattatcc attgccccct ccctcgcgct ggaacggata
     5641 aaccgaaccg agattcagta attggctcaa tacatccagt gcttcccgcc cttcgcgcag
     5701 caattgcggg tcggccagat cggcagcagt aagacgatcg cggtagtaac gatccaccca
     5761 gtcattgagc gcattaaaca aggtatcgtt catcatcacc gccggattca ccgcccggcg
     5821 ttcttcttct gtcaatacca cccgcaaccg caggcacgcc gggccgccgc cattcgccat
     5881 gctttcacgt aagtcaaaga cttttagttc gctaatcggg ttgtcagcgg caaggagttc
     5941 attgagataa ccccatactc cggcgtgttc ccgacactcc tgaggcagca ccaacatcat
     6001 ggaaccatca tcgcggctca acagttggct gttaaacaga taggtagaca ccgcatccga
     6061 cacggaaacc tgagttgccg gaacttctat cgccataaaa ccgttcaccc gcgcccgcag
     6121 gtttgccagt aactgtgact ggcgagcgaa cgcctgttgg tggcaaaaca gcacctggcg
     6181 gttacttacg gcaatcacgt cattatgaaa aacaccctgg tcgataacgt ccgggttttg
     6241 ctgggcgaaa atcacctgtt ggggattcac ctgattaagc cttgccaccg cctcgctggc
     6301 ttcgcgagtc tgtcgcgccg gataacggga aggccgggta tcattgcctt cttctcgccc
     6361 gtagacaaaa agttgcatac ccggctcacc gtaatgaccg ccgagacgat tgtggtttgc
     6421 cgccccctca tcaccgagca acgctacctg cggcaacgcc gaatggacgc taaatttctc
     6481 ttcgtcgtta aaaatagctt ttaacagcga ttcagtgacg ggcgcttcca gcgaacggtg
     6541 aaatttattg ttcaggttgg caacggtgag atgcactttg ccatccagcg tatcggcaga
     6601 tggcgcgatc gttgccgcat tggctaccca cattggcgaa gcggagctga cgctggaaag
     6661 ccagtgcggt gcctggcgtg caactttttc cagtacctgc tcatcgctac cactgaatcc
     6721 caactgacgc agcaccggaa taaacggacg ctcgtgcggc gggatcacgg cctgggggaa
     6781 tcctgcatcg gcaagggttt tcattttcag taagccctgt ttcgccgcca gtcgcgggtt
     6841 agacacctga aaacggtgac gggtagaggc ttcattacca aacgacaggc cagcgtaatg
     6901 atgcgtcagc cctaccagcc cgtcgaaatt aacttcccag gcgttcatcg caccacctca
     6961 tcggaaaaat ccagcccggg gttaagcgtt gcgggcaaag ttaacgagtc cgactccagg
     7021 ctcgccatag gccatgcgca gtaatctgcg gcataccagg cgctggggcg atggttaccg
     7081 gatgcaccaa tgccgccgaa tggcgcggta ctggcagcac cagtaagcgg tttgttccag
     7141 ttaacaatcc ccgcccgcgc ctccagcaac agttgatcga atttttcccg ttcgggggaa
     7201 accagaccgc aagagagtcc gaagcgagtg ttattcgcca ttagaatcgc ttcctcgaaa
     7261 gaatcataac gccagacgcg caataacggt ccgaacacct cttcatctgg tacgccagca
     7321 acgcctgtca tttcaatgat ccccggcgtc agcaacgatg tctctaattg taataagcgc
     7381 ggcgcaagca gggttcgtcc gcccatcgct tccagttgct gccaggcagt aaccacctgc
     7441 tgtgcggcct gttcagaaat cagcccgcca ataaacggct gcggttcgtc atcccagttg
     7501 cccggcgtta atcgctggct gacggcaacc agacgagcaa gaaacgcatc gccctgcgct
     7561 ccgcttttga gcaataaacg gcgggcgcag gtgcagcgtt gcccggctgt gacaaacgcc
     7621 gactgaatgg tcagatggac agccgcgtcg atatccgcca cctcatcgat aattagcgga
     7681 ttattaccgc ccatctcaag ggcgagaatt ttctccggct gaccggagag ctggcgatgc
     7741 agctggtagc cggtattggc gcgaccggta aacagcaaac cgtcgagatc ctccagcgca
     7801 ctcagcgcct gacccgtttc acgcccgccc tgcaccaggt tcagcacgcc cggcggcaag
     7861 ccagcctgct gccataaacg cattaccgct tcgccactcc acggtgtcag ttcgctgggt
     7921 ttaaagataa tggtgttacc tgccagcaat gccggaacga tatgtccgtt cggcaaatga
     7981 ccagggaaat tatacggccc aaacaccgcc agcacgccgt gcgggcgatg tcgcaggctc
     8041 gccgcaccgt ccggcatttc actacgctgc tcgccggtac gaacgtgata cgccttaatt
     8101 gatatcgcga ttttattgat catcgccgtc acttcggttg ccgcttccca gcgcagctta
     8161 cccgtttctc tggcaataat cgcggttaat tcggctttat tgctttccag caatccggca
     8221 aagcgttcga caacgacctg acgttcagcc aatgagagcc gcgcccagcg cggaaacgcc
     8281 gcacgggctg cccgacaagc ctgttcgacc tgagcggcat cggcatcatt gccttgccat
     8341 aacacctcgc ccgataccgg attacgcttc acacgcgatg cgccctggcc cgttatccag
     8401 tcaccgttaa tccataaagt catgctgttt tctccactgc gcacaggcgc accagacgaa
     8461 cgcgatcccc tgcgtggcat ttgagggcat ccagttgtgc ggcggttaaa atcaaacgct
     8521 cggttgccgg atcggtacgc gccagcacca cgcggaaatg gtgataattt tcattggcga
     8581 ccaggcaggc cggaaaatcg ccctgcgcag gctgcccttc tgccacttcc accagccgac
     8641 ttttacggat ggcgcgcacg cggtcgatgt cacactcaag cgttggcccg ccgtcgaaga
     8701 tgtcgatata gttgcggtaa cgaaaacctt ctttctccag caccgcgcgg gcaggcgcgg
     8761 tttgcggatg tacctgaccg atgacgtcct gggcttcctg ggataaaaag tgggtataga
     8821 tcggatgttt cggcatcagt tctgcaataa atgccttttg cccggtgccg cagagaaaat
     8881 cggcgcggct aaaatccatc gaaaagaagc gtttaccgag gctttgccag aacggtgaat
     8941 agccgtgttc gtcaatcacc ccgcgcattt cagcaaccac tttgtcatta aacttgtcgc
     9001 gaaaagccgc cataaacata aagcgcgatt tcgacagcaa atagccgttg ccctctttgc
     9061 gccagtccgg gtcgagaaac agcgtgcaca gctcgctgct gccggtgtga tcgttactaa
     9121 gaaacagcgt cggcaatgcg ttatagacat tcagctcttt tgaggcgtga accaacgtgc
     9181 cgacccgata gttgtgccag ggatcgttca gcccaaccgc cacctcaatg gcgcaaatcc
     9241 ccgccacggt gcctgtctcg ctatcttcca gcacgaacac atagccctgc tcgcttttgg
     9301 gcagttcgcc ttgccaggtt ttgattgccc tttcgatacg cgcggaaagc gtggcttcat
     9361 tagcgggaag cgacgtcagg ccgccgcccg ttttgctggc aagctgcatc agcgccgaga
     9421 catctgagcg ctcaacggga cggatgacca tcatgatgaa cctcggctaa caaaatgttc
     9481 gcaagcagct gcaaagcgat ccagtccggt cgtcacctct tcttcgctga cattgagcgc
     9541 aggcgcaaaa cgcaccacgt tgccacccgc aatcagtacc ataacgcctg ctttcgccgc
     9601 ctcctgagag atctgtttcg cttgtccggc gtaatcggca ttcagtacac agccaatcaa
     9661 caaacctaag ccgcgaactt cactgaacaa accgtagcgg tgattaatgg tattaagacg
     9721 ctcaacaaac cagtcgtgac gctgtttaac gccattaagc atctctggtg tgttgatgag
     9781 ctccagcact ttgcccgcca ccgccgaggc cagcggattg ccgccatagg tggtgccatg
     9841 agtgccaacg gtcatcacgc tggcgcactc ttcggttgtt aacaacgcac cgaccgggaa
     9901 atcgccgccc agcgctttgg cggtagttaa cagatcaggt gtcacgccgt agtgcatata
     9961 ggcatacagt tccccggtgc gcccgacgcc ggtttgtact tcatcaaaaa tcaacagcgc
    10021 attgtggcgg tcacacaatt cacgcagacc ttgtaaaaac gcgttgctgg ctggcaccac
    10081 accgccttcc ccctggatgg gttcgacaat caccgcacag gtggcgtcgt caatcagcgc
    10141 gctggcagag ttaatatcgt tatatgcagc atgacggata tctggtggca gtggcgcaaa
    10201 atcctgtgaa taggctggct gcccacccgc actgacagta aacagcgtgc gaccatgaaa
    10261 cgcattttta aacgccatga taccgctctt atggctgccg tagcggtcgt gagcgaattt
    10321 acgcgccagt tttagcgccg cttcgttggc ttccgcaccg gagttacaaa agaagacgcg
    10381 atcggcaaac gtggcgtcga tcaatttttt cgccagtcgc agtaccggct cgttggtgta
    10441 accgttgccg gtatgccaga acttactcgc ctgttcgttc agcgcttcac gcagttccgg
    10501 atgcgcatgg cccagcgcgt tcaccgcaat gccacccgcg aagtcgatat actctttccc
    10561 ctgctgatcc cacaagcgcg aaccttcgcc acgtaccggt ataaagggtg ccggagcgta
    10621 aacaggtatc atccattcat caaagttttc acgcgtaatt ggctgagaca tagcgacctc
    10681 tacagtaaat aattcgttat ttatatgtta acaataagta atgtttgcgc tgtaaatgta
    10741 gattgcaggg ttcgtgccag ccagtgataa aagtgcataa acggcggagg ctaactggaa
    10801 atcaaggagt tataaccaaa ccatatgcat ttaaagtgca tataaagtga atacgtttgc
    10861 gatgtgggtg aataaaaaga ataaaaaacg caatgttatg cagaagtaaa atataattct
    10921 ggaattgtga tcattgacga aatttactgg aaattactgc gccattctga cgcagcgcgc
    10981 accaaaagcg ggcatttttt gcgccatcgt tgacatcatt aacaaccatc gatcaaatca
    11041 cttaacaaca ggcggtaagc aacgcgaaat tctgctacca tccacgcact ctttatctga
    11101 ataaatggca gcgactatga aatttgtctc ttttaatatc aacggcctgc gcgccagacc
    11161 tcaccagctt gaagccatcg tcgaaaagca ccaaccggat gtgattggcc tgcaggagac
    11221 aaaagttcat gacgatatgt ttccgctcga agaggtggcg aagctcggct acaacgtgtt
    11281 ttatcacggg cagaaaggcc attatggcgt ggcgctgctg accaaagaga cgccgattgc
    11341 cgtgcgtcgc ggctttcccg gtgacgacga agaggcgcag cggcggatta ttatggcgga
    11401 aatcccctca ctgctgggta atgtcaccgt gatcaacggt tacttcccgc agggtgaaag
    11461 ccgcgaccat ccgataaaat tcccggcaaa agcgcagttt tatcagaatc tgcaaaacta
    11521 cctggaaacc gaactcaaac gtgataatcc ggtactgatt atgggcgata tgaatatcag
    11581 ccctacagat ctggatatcg gcattggcga agaaaaccgt aagcgctggc tgcgtaccgg
    11641 taaatgctct ttcctgccgg aagagcgcga atggatggac aggctgatga gctgggggtt
    11701 ggtcgatacc ttccgccatg ctaatccgca aacagcagat cgtttctcat ggtttgatta
    11761 ccgctcaaaa ggttttgacg ataaccgtgg tctgcgcatc gacctgctgc tcgccagcca
    11821 accgctggca gaatgttgcg tagaaaccgg catcgactat gaaattcgca gcatggaaaa
    11881 accgtccgat cacgcccccg tctgggcgac cttccgccgc taatttagca gctctcctgg
    11941 ctcaaactgg gtcaggagaa ttaaccttga gaaaaatcaa caaactgtca gtaatgattt
    12001 gttgcctgcc gtcctttgtt ataccgtcac tgcgttttta gttgtctgac cacttctcta
    12061 ttatcaagtt tgatatagga aactccacga tgaatgctga gcgtaaattt cttttcgcct
    12121 gtcttatttt tgcgctggtc atttacgcta tccacgcttt cggtttattc gatctgctca
    12181 ccgatttacc ccacttacag acactcattc gccagagcgg attgttcggt tatagcctct
    12241 atattctgtt attcatcatt gccaccctct ttctgttacc aggaagcata ctggtgatcg
    12301 ctggaggaat agtttttggc ccgctcttag ggacactact ctcattaatt gccgccacgc
    12361 tggcctcctc gtgctcattc ctgctggcgc ggtggctggg acgtgattta ctgctgaaat
    12421 acgtgggcca tagccatacc ttccaggcca ttgaaaaagg cattgcgcgt aacggtattg
    12481 attttcttat tctgacccgc ttaatcccgt tgtttcctta caatattcaa aattacgctt
    12541 acggattaac caccatcgcc ttctggcctt atacccttat ttcggcactc acgaccctgc
    12601 ccggtattgt tatttatacc gtgatggcaa gcgatctcgc caatgaaggc attacgct
//
LOCUS       DAJTMC010000122        12602 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.122, whole genome shotgun sequence.
ACCESSION   DAJTMC010000122 DAJTMC010000000
VERSION     DAJTMC010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12602
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.122"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(14..520)
                     /gene="mug"
                     /locus_tag="OO130_003017"
     CDS             complement(14..520)
                     /gene="mug"
                     /locus_tag="OO130_003017"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HCR5366337.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            645..720
                     /locus_tag="OO130_003018"
     tRNA            645..720
                     /locus_tag="OO130_003018"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:678..680,aa:Ile,seq:cat)
     gene            complement(774..1538)
                     /gene="nfeF"
                     /locus_tag="OO130_003019"
     CDS             complement(774..1538)
                     /gene="nfeF"
                     /locus_tag="OO130_003019"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
                     /protein_id="HCR5366338.1"
                     /translation="MNNSPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHDGGVASGWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
     gene            1826..2449
                     /gene="nfeR"
                     /locus_tag="OO130_003020"
                     /gene_synonym="yqjI"
     CDS             1826..2449
                     /gene="nfeR"
                     /locus_tag="OO130_003020"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HCR5366339.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            complement(2603..4123)
                     /gene="aer"
                     /locus_tag="OO130_003021"
     CDS             complement(2603..4123)
                     /gene="aer"
                     /locus_tag="OO130_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HCR5366340.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLAAMLWFVAAPVVTYFLCVLVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSIIASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDVSADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            4540..5919
                     /gene="ygjG"
                     /locus_tag="OO130_003022"
     CDS             4540..5919
                     /gene="ygjG"
                     /locus_tag="OO130_003022"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HCR5366341.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGSLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLLAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            complement(5961..6293)
                     /locus_tag="OO130_003023"
     CDS             complement(5961..6293)
                     /locus_tag="OO130_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HCR5366342.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRVV"
     gene            6512..7495
                     /gene="ebgR"
                     /locus_tag="OO130_003024"
     CDS             6512..7495
                     /gene="ebgR"
                     /locus_tag="OO130_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HCR5366343.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLGIELTN
                     CYEHSGLPDIKNVTGILIVGKPTPALRAAACALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAQEDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            7679..10771
                     /gene="ebgA"
                     /locus_tag="OO130_003025"
     CDS             7679..10771
                     /gene="ebgA"
                     /locus_tag="OO130_003025"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HCR5366344.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLGNLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDHGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGPGLKEYKQVIAPVKIHARDLTRGELKVENKLWFTT
                     LDDYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSR
                     TRYSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKM
                     SGKPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQ
                     SDGEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFT
                     MGINGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRW
                     TALTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLG
                     SNSWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ
                     "
     gene            10768..11217
                     /locus_tag="OO130_003026"
     CDS             10768..11217
                     /locus_tag="OO130_003026"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HCR5366345.1"
                     /translation="MRIIDNLEQFRQIYASSKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            11280..>12602
                     /locus_tag="OO130_003027"
     CDS             11280..>12602
                     /locus_tag="OO130_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5366346.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVG"
BASE COUNT         3014 a         3407 c         3171 g         3010 t
ORIGIN      
        1 tcggtggcgt tttttatcgc ccacgcacta ccagcgcctg gtccagctcg cgatacgctt
       61 caaccagttt ctctagtgaa acgcgactta aaccgctggg atttggcagc acccaaatct
      121 gcgtcgaacc aatggtgagc gtttgtttcc cccactgtgc accgcgctgg ctgaatccct
      181 gttcatatgc ttgtttgccc agaatcgcca acgcctgcgg ctgataatct tcaatttttt
      241 caatcagctt acgcccacct gcgtgcagct cctgctttga aacttcattg gcttgcaccg
      301 ttggacggtc taccagtttg gtgacgccac aacgataatc cagcagatgc tgtgcctcct
      361 gcggcttcaa ctgacggtcg gtaaacccgg cctgatatat caccttccag aagcgatttg
      421 ctggatgagc aaagggaaaa ccagtcccgg cggatgaaag cccagggttg ataccgcaaa
      481 acacgacccg taaccctgga gccaaaatat cctcaaccat atttactcct ttctaacatc
      541 accaggaaaa gtataacgga ttgaaaatac attgtttata aaaacagcag gcgcacggta
      601 atggctggat tgcgacacgg agttacttta taatccgcta ccatggcccc ttagctcagt
      661 ggttagagca ggcgactcat aatcgcttgg tcgctggttc aagtccagca ggggccacca
      721 gatatagcaa aggctgacga gaaatcgtca gcctttttct ttttatatct cagttacttt
      781 gcgtgccagt aagccgctgc acgtacccgc tgtgggtcat actgttccgc ttcaaagcgg
      841 cggcttaaat tcttaacgac tttaccttcg ccggttatcc agatgaagta atcatcggca
      901 gggatttgca tctgcgccag acgcgcatct accgcctgct catcatgtgc cagccattcg
      961 atattaaaac catcaaggtg cgcgagataa tcctgacagg cgttatcccg cacgctaacc
     1021 agcgcactaa cttgcggttt aacggcaagt ttgctcaacg tttccaggcg gcggcgcagt
     1081 gcgggcattc cggattcatc gcagacatac agctgatacg cgtaatcttc cggcaccacc
     1141 agcgaaccgc gcggaccagc caccgtaagt ttatcgcccg gttgcgcctg catcgcccag
     1201 ccgctggcga ccccaccgtc gtgaataaag aaatcgatcg ccagttcatg gcgtagttcg
     1261 tcatacagcg gcgtatagtc acgcgacggt gggcgtggtc cttccggcca gacgatgccc
     1321 tcttccgtta ccgttggcgg cacaaagtga gcgtcaggtt gaggaaagaa gagtttgctg
     1381 tgatcgtcaa agccgcgcga tgtaaaaccg tccagcgcct cgccgccgag gacaatgcgc
     1441 tgaaaaccgg cgctgatgcg ctcaacgcgt aacacagtca gttcacggaa gcgcagatca
     1501 ttgcgaacgc gctgcgggta gcggggggag ttattcattg ttatcgcctt cgtgatggta
     1561 atcagatata tctaaataat tttcgtaaat gataatgatt gttaatcatg ataaatgcaa
     1621 gcgattagta gaactgatat gtctatagtc tgataagacg aaccgcctct tctcaggcat
     1681 cattactcaa cgccggatgc ggcgtgaacg ccttatccgg cctacgtgtg agatgagtcc
     1741 acatttaaaa atcattttta ttcttgcaag agtgctcata tcagatataa attagatata
     1801 tctaatcaag aaaaaggagg ctgatatgag ccatcatcac gaagggtgtt gtaaacatga
     1861 aggccagcca cgccatgagg gctgctgcaa aggtgagaag tcagaacacg agcactgcgg
     1921 acacggtcac cagcatgaac acggtcaatg ctgcggtggt cgccacggtc gcggcggcgg
     1981 tcgtcggcaa cgtttctttg gtcacggtga attgcgtctg gtgattctgg atattctctc
     2041 gcgcgatgac agccacggtt acgaattgat taaagcgatt gagaatctaa cccaggggaa
     2101 ttacacccca agcccgggcg tcatctaccc aacgctggat tttctgcagg agcagtcgct
     2161 gattaccatc cgcgaagagg aaggtggtaa gaagcagatt gcgctgaccg aacaaggcgc
     2221 gcagtggctg gaagaaaacc gcgaacaggt ggagatgatt gaagaacgca tcaaagcgcg
     2281 ttgcgttggc gcggcgctgc gccagaaccc gcaaatgaag cgggcgctgg ataattttaa
     2341 agcggtgctg gatttacgcg tcaaccagag cgatatcagt gatgcacaaa taaaaaagat
     2401 cattgcggtg atcgaccgcg ccgcttttga tattacgcaa ctggattaat cgccgcatcc
     2461 gccagtggcg cggtgcaatt gccggatgcg acgcttgacg cgccttatcc ggcctacacc
     2521 cactacacac cccgcaggcc tgataagatg cgccagcatc gcatcaggca ttgtgctcca
     2581 accgccggat ccggcatacc gattaatgca gcaccgtcac cgcgtcttcc agtcggctgg
     2641 cgcggtgttt caccatcgcc gacacctgcg cactctcttc caccagctca gcatttttct
     2701 gggtgatcag gttaagctca tccactgcac gggtcaggct ggaaagccca tcggcctgtt
     2761 ccagcgttga atggctaatc tgggcgatca actgggtgac gtttttcacc tgtgccacaa
     2821 tatcttccat cgtccgtccg gcggcgtgta cctgctgcga accggattgc accttatcag
     2881 cactgacatc aatcagcttg cgaatatcgt tggcagcatt agcgctgcgg ctggctaaat
     2941 gacgcacttc ccctgccacc actgcaaaac ctttgccctg ttcgccggca cgcgccgctt
     3001 ccaccgccgc attcagggcc agaatattgg tctgaaacgc aatatcgtta atcagcgaag
     3061 taatggtgcc aatgcgctgg gtactgtcgg cgatatcgtc cattgtcttg atcaccgtgg
     3121 tcatcgcctc cccaccctgc actgccgcat tactggcgat gatagaaagt ttatcggcag
     3181 ccgacgccgt ggcagagttc tgtttcaccg acgccgccat ttggttcatg gtcgccaccg
     3241 tttgctgaac gttatcaact gtctgctggg tatgttcgtt cagttcatcg gtgcctttcg
     3301 ccagcgtctc actgccattt ctgacactgg acacctggct tgagacatcg ttaattagcc
     3361 aacggcacat caggccaagc tgccctaccg cgcgtaatgt cagccccagc tcatcgctgc
     3421 gattcaggtg ctcaacacta ttacgctctc cggtcgccac cttcagtgcc tgacgggcga
     3481 cattttctat cgggcgcaca atctgccatt caaaacaagc gcttgccaac aataccacta
     3541 acacacagag gaaatacgtc accaccgggg cagcaacaaa ccaaagcatg gccgccagca
     3601 agataaacat cagggtcatc actccacgcg cccgccagcg aagcggtaat gaaggcagtt
     3661 tacccagcca gcctttacgc accaccaggc ctttatgaat acgcttactg gtacgtccgg
     3721 cgttcagcgc tttgtacagc ggctccaccg ccgcaatctc ttcatccgtc gcccgggtac
     3781 gaatcgacat atagccactg atttttccct cgcgcaccat cggtaccgca ttggcccgca
     3841 cccaataatg gtcgccattt ttgcggcgat ttttcacgat gccgctccag ggctcccctt
     3901 ttttcagggt gaaccacata tccgcaaacg ccgcttttgg catatccggg tgacgcacca
     3961 tgttgtgcgg ctgcccttgt aactcttgca aggtaaagcc gctcacctgc acaaaagtgt
     4021 cattagcatg agtgatatag ctttgcagat cggtagtgga catcagagtg gtatcgtccg
     4081 ccagcggggt attttgctgg gtgacatacg gatgagaaga catgttcgcg tcctatgcag
     4141 gttatatggt tgttaacttc ttgtcagagt ttatgtcggc cccgctgcgg ttatctttaa
     4201 ccgattaatt tgatttagat cgcaatttgc gattaaagca caaatctaat tccttgattt
     4261 aaaatacttt cactctgtta ctatacgaaa acgttaatta tcttgcccaa aatcaggcaa
     4321 ttatcgccct gaaaacgtgc atttgcacag caatcatcaa atccataccc gacaaaaacc
     4381 gtgcaaaata acaacgaatg ttaacaaata gcattaaata ctgcgcaaat gataaccaca
     4441 tttgtgttta tcccgatttt cgcgatcgca gccggagtgg cgcaatccct gcaatactta
     4501 aatcggtatc atgtgatacg cgagcctccg gagcatattt tgaacaggtt accttcgagc
     4561 gcatcggctt tagcgtgcag cgcccacgcc ctgaatctca ttgagaagcg aacgctggat
     4621 catgaggaga tgaaagcact taaccgagag gtgattgaat acttcaaaga gcatgtcaat
     4681 ccggggtttt tagagtatcg caaatctgtt accgccggcg gggattacgg agccgtagag
     4741 tggcaagcgg gaagtttaaa tacgcttgtc gacacccagg gacaggagtt tatcgactgc
     4801 ctgggaggtt ttggaatttt caacgtgggg caccgtaatc cagttgtggt ttccgccgta
     4861 cagaatcaac ttgcgaaaca accgctgcac agccaggagc tgctcgatcc gttacgggcg
     4921 atgttggcga aaacccttgc tgcgctaacg cccggtaaac tgaaatacag cttcttctgt
     4981 aatagcggca ccgagtccgt tgaagcagcg ctgaagctgg cgaaagctta ccagtcaccg
     5041 cgcggcaagt ttacttttat tgccaccagc ggcgcgttcc acggtaaatc acttggcgcg
     5101 ctgtcggcca cggcgaaatc gaccttccgc aaaccgttta tgccgttact gccgggcttc
     5161 cgtcatgtgc cgtttggcaa tatcgaagcc atgcgcacgg ctcttaacga gtgcaaaaaa
     5221 accggtgatg atgtggctgc ggtgatcctc gaaccgattc agggtgaagg tggcgtaatt
     5281 ctgccgccgc cgggctatct caccgccgta cgtaagctat gcgatgagtt cggcgcactg
     5341 atgatcctcg atgaagtaca aacgggcatg gggcgcacgg gcaagatgtt cgcctgcgag
     5401 catgagaacg tacagccgga tatcctctgc cttgccaaag cgctcggcgg cggcgtgatg
     5461 ccgattggcg cgaccatcgc cactgaagag gtgttttcag ttctgttcga caacccattc
     5521 ctgcatacca ccacctttgg cggcaacccg ctggcctgtg cggcggcgct ggcgaccatc
     5581 aatgtgttgc tggagcagaa cttactggct caggctgagc aaaaaggcga tatgttgctg
     5641 gacggtttcc gtcaactggc gcgggaatat cccgatctgg tacaggaagc gcgtggtaaa
     5701 gggatgttga tggcaattga gtttgttgat aacgaaatcg gctataactt tgccagcgag
     5761 atgttccgcc agcgcgtact ggtggccgga acgctcaata acgccaaaac gatccgcatt
     5821 gaaccgccac tgacactgac cattgaacag tgtgaactgg tgatcaaagc ggcgcgtaag
     5881 gcgctggcgg ccatgcgagt aagtgtcgaa gaagcgtaat atcaaatcgg atggcgatac
     5941 gacgtcgcca tccgatcttt ttatacgacg cgtacgcccg caggcatcat ccgctccggc
     6001 gttaacaaca cgctttcact gccgtcatcg gtttccgcac acaacagcat gcattccgac
     6061 gtttcaccac gcattttcgc tttttgcaga ttgcataaga ccaccaccgt tttccccatc
     6121 agctcttctt cgctgtagta cggcaccagg ctggtcacgg tttgcagtgt tttttccccc
     6181 acatcaacct gtacgatgta cagcttgtcg gcgttttcat ggcgtttcac ttccacaatc
     6241 tttccgacgc gcatttccag acgtgcaaaa tcagcgtaag ccacggtttc cattgtttcc
     6301 ttccttcggg taaaatttta ctaaactata gcaaaagttt ttctcaatcc tgtaggctaa
     6361 aaatggagaa tgcaggcgtg atcacattcg tgagccgctg tgttaccgtt acagcgtcaa
     6421 agaaacgcgc tttatttact gaaaacaggt gacccgataa gtacttcctc tacaatgggg
     6481 gcgcacatca gggaaagtaa aaaaggtaaa catggcaaca ctaaaagaca tcgcaatcga
     6541 agctggcgta tccctggcga cagtatccag ggtcttaaat gacgatccga cattgaatgt
     6601 gaaagaagag acgaaacatc gcattctcga gatcgccgaa aagctggagt acaagaccag
     6661 tagtgcccgc aaactccaga caggtgcagt caaccaacac catattctgg ctatctacag
     6721 ctaccaacag gagctggaga tcaacgatcc ttactatctg gcgatccgcc acggcattga
     6781 aactcagtgc gaaaagctgg gcattgagct caccaactgt tatgaacaca gcggattacc
     6841 agacattaaa aacgtcaccg gtattttaat tgtcggcaaa cccacgcccg cccttcgcgc
     6901 cgccgcctgt gcgttgaccg acaatatctg ttttatcgac tttcacgaac ccggcagcgg
     6961 ttacgatgcg gtggatatcg atctggcacg catcagtaaa gaaatcatcg acttctatat
     7021 caatcagggc gttaatcgta ttggttttat tggcggtgaa gatgagccag gcaaagcgga
     7081 tattcgtgag gtcgcctttg cggaatatgg ccgactgaaa caagtggtac gcgaagagga
     7141 tatctggcgc ggaggctttt ccagttcgtc gggttatgaa ctggcaaaac agatgctggc
     7201 gcaggaagac tatccgaagg cactgtttgt tgcttccgat tccattgcta tcggcgtact
     7261 gcgggcaatt catgaacgag gcctgaacat cccacaggat atttcgctta tcagcgttaa
     7321 cgatatcccc accgcgcgat ttacctttcc gccgctctcc accgtgcgca tccattccga
     7381 gatgatggga agtcagggcg ttaacctggt gtatgaaaaa gcccgcgatg gtcgcgcgct
     7441 gccgctgtta gtcttcgttc ccagcaaatt aaaactgcgc ggcacgaccc gttaaatccc
     7501 cttacacact gtccggcaat cgtttttgcc ggacagtgct gccgtttatt ttcgtgatcc
     7561 agttaaagta aatgcattta tctgctactt tttagtaaaa attttactga actccccagc
     7621 aattacacaa actaccatca ccatgaatgg ttccgatttc tctctaccgg gaggccctat
     7681 gaatcgctgg gaaaacattc agctcaccca cgaaaaccga cttgcgccgc gtgcgtactt
     7741 tttttcatat gattctgttg cgcaagcgcg tacctttgcc cgcgaaacca gcagcctgtt
     7801 tctgccctta agcggtcagt ggaatttcca cttttttgac catccgctgc aagtaccaga
     7861 agccttcacc tctgagttaa tggctgactg ggggcatatt accgtccccg ccatgtggca
     7921 aatggaaggt cacggcaaac tgcaatatac cgacgaaggt tttccgttcc ccatcgatgt
     7981 gccgtttgtc cccagcgata acccaaccgg tgcctatcaa cgtattttca ccctcagcga
     8041 cggctggcag ggtaaacaga cgctgattaa atttgacggc gtcgaaacct attttgaagt
     8101 ctatgttaac ggtcagtatg tgggtttcag caagggcagt cgcctgaccg cagagtttga
     8161 catcagcgcg atggttaaaa ccggcgacaa cctgttgtgt gtgcgcgtga tgcagtgggc
     8221 ggactctacc tacgtggaag accaggatat gtggtggtca gcggggatct tccgcgatgt
     8281 ttatctggtc ggaaaacacc taacgcatat taacgatttc actgtgcgta ccgactttga
     8341 cgaagcctat tgcgatgcca cgctttcctg cgaagtggtg ctgggaaatc tcgccgcctc
     8401 ccctgtcgtc acgacgctgg aatataccct gtttgatggc gaacgcgtgg tgcacagcag
     8461 cgccattgat catttggcaa ttgaaaaact gaccagcgcc agctttgctt ttactgtcga
     8521 acagccgcag caatggtcag cagaatcccc ttatctttac catctggtca tgacgctgaa
     8581 agacgccaac ggcaacgttc tggaagtggt gccacaacgc gttggcttcc gtgatatcaa
     8641 agtgcgcgac ggtctgttct ggatcaataa ccgttatgtg atgctgcacg gcgtcaaccg
     8701 tcacgacaac gatcatcgca aaggccgcgc cgttggaatg gatcgcgtcg agaaagatct
     8761 ccagttgatg aagcagcaca atatcaactc cgtgcgtacc gctcactacc cgaacgatcc
     8821 gcgtttttac gaactgtgtg atatctacgg cctgtttgtg atggcggaaa ccgacgtcga
     8881 atcgcacggc tttgctaatg tcggcgatat cagccgtatt accgacgatc cgcagtggga
     8941 aaaagtctac gtcgagcgca ttgttcgcca tatccacgcg cagaaaaacc atccgtcgat
     9001 catcatctgg tcgctgggca atgaatccgg ctatggctgt aacatccgcg cgatgtacca
     9061 tgcggcgaaa gcgctggatg acacgcgact ggtgcattac gaagaagatc gcgatgctga
     9121 agtggtcgat attatttcca ccatgtacac ccgcgtgccg ctgatgaatg agtttggtga
     9181 atacccgcat ccgaagccgc gcatcatctg tgaatatgct catgcgatgg ggaacggacc
     9241 gggcgggctg acggagtacc agaacgtctt ctataagcac gattgcattc agggtcatta
     9301 tgtctgggag tggtgcgacc acgggatcca ggcacaggac gaccacggca atgtctggta
     9361 taaattcggc ggcgactacg gcgactatcc caacaactat aacttctgtc ttgatggttt
     9421 gatctattcc gatcagacgc cgggaccggg cctgaaagag tacaaacagg ttatcgcgcc
     9481 ggtaaaaatc cacgcgcggg atctgactcg cggcgagttg aaagtcgaaa ataaactgtg
     9541 gtttaccacg cttgatgact acaccctgca cgcagaggtg cgcgccgaag gtgaaacgct
     9601 cgcgacgcag cagattaaac tgcgcgacgt tgcgccgaac agcgaagccc ccttgcagat
     9661 cacgctgccg cagctggacg cccgcgaagc gttcctcaac attacggtga ccaaagattc
     9721 ccgcacccgc tacagcgaag ccgggcattc tatcgccact tatcagttcc cgctgaagga
     9781 aaacaccgcg cagccagtgc ctttcgcacc aaataatgcg cgtccgctga cgctggaaga
     9841 cgatcgtttg agctgcaccg ttcgcggcta caacttcgcg atcaccttct caaaaatgag
     9901 tggcaaaccg acatcctggc aggtaaatgg cgagtcgctg ctgacccgcg agccaaagat
     9961 caacttcttc aagccgatga tcgacaacca caagcaggag tacgaagggt tgtggcagcc
    10021 gaatcatttg cagatcatgc aggaacatct gcgcgacttt gccgtagaac agagcgatgg
    10081 tgaagtgttg atcatcagcc gcacagttat tgcaccgccg gtgtttgact tcgggatgcg
    10141 ctgtacctac atctggcgca tcgctgccga tggccaggtt aacgtggcgc tttccggcga
    10201 gcgttacggc gactatccgc acatcattcc gtgcatcggt ttcaccatgg ggattaacgg
    10261 cgaatacgat caggtggcgt attacggtcg tggaccgggc gaaaactacg ccgacagcca
    10321 gcaggctaac atcatcgata tctggcgcag caccgtcgat gccatgttcg agaactatcc
    10381 cttcccgcag aacaacggca accgtcagca tgtccgctgg acggcactga ctaaccgcca
    10441 cggcaacggt ctgctggtgg ttccgcagcg cccaattaac ttcagcgcct ggcactatac
    10501 ccaggaaaac atccacgctg cccagcactg taacgagctg cagcgcagtg atgacatcac
    10561 cctgaacctc gaccaccagc tacttggcct cggctccaac tcctggggca gcgaggtgct
    10621 ggactcctgg cgcgtctggt tccgtgactt cagctacggc tttacgttgc tgccggtttc
    10681 tggcggagaa gctaccgcgc aaagtctggc gtcgtatgag ttcggcgcag ggttcttttc
    10741 cacgaatttg cacagcgaga ataagcaatg aggatcatcg ataacttaga acagttccgc
    10801 cagatttacg cctctagcaa gaagtggcaa cgctgcgttg aagcgattga aaatatcgac
    10861 aacattcagc ctggcgtcgc ccactccatc ggtgactcat tgacttaccg cgtggagaca
    10921 gactccgcga ccgatgcgct atttaccggg catcgacgct attttgaagt gcattactac
    10981 ctgcaagggc agcaaaaaat tgaatatgcg ccgaaagaga cattacaggt agtggaatat
    11041 tatcgtgatg aaactgaccg tgaatattta aaaggctgcg gagaaaccgt tgaggtccac
    11101 gaagggcaaa tcgttatttg cgatatccat gaagcgtatc ggtttatctg caataacgcg
    11161 gtcaaaaaag tggttctcaa agtcaccatc gaagatggtt atttccataa caaataacaa
    11221 ctacggcggc aaaaggagtt tgccgccacc gctaccctac acattttcgg agatgtgtta
    11281 tgtctgatac caaacgtaat acaatcggca aattcggctt gctctcgctg acttttgctg
    11341 ccgtttacag ctttaacaac gttatcaata ataatattga gcttggactg gcctcggcac
    11401 cgatgttttt cctcgcgacg attttttatt ttattccctt ctgtctgatc atcgcagaat
    11461 ttgtttcgtt aaataaaaac tcagaagccg gtgtctacgc gtgggtaaaa agttcgctgg
    11521 gcggacgttg ggcatttatt actgcctata cctactggtt cgtaaacctg ttctttttca
    11581 cctcactgtt gccgcgcgtt attgcttatg cttcgtatgc cttcctcgga tacgaatata
    11641 ttatgacgcc ggttgccacc accattatca gtatggtgct gttcgccttc tccacctggg
    11701 tttccaccaa cggggcgaaa atgttggggc caattacctc cgtcacttca acgctgatgc
    11761 tgctgttaac gctctcctac attttactgg caggtacggc gctggttggc ggcgtacagc
    11821 ctgctgaccc catcaccgtt gacgcgatga tcccgaactt caactgggcg ttcctcggcg
    11881 ttaccacctg gatctttatg gccgcaggtg gcgcggagtc cgtcgctgtg tacgttaacg
    11941 acgtcaaagg cggttcgaaa tcgttcgtta aagtgatcat cctcgccggg atttttatcg
    12001 gcgtactgta ttccgtctcc tcggtgctga ttaacgtctt cgtcagcagc aaagagttga
    12061 aatttaccgg cggatcggtg caggtattcc atggcatggc ggcgtatttt ggtctaccgg
    12121 aagcgttgat gaatcgcttt gtcggtctgg tgtcctttac cgcgatgttc ggttccctgc
    12181 tgatgtggac cgcaacgccg gtgaaaattt tcttctccga aatcccggaa ggcatctttg
    12241 gtaagaaaac cgtcgaactg aacgaaaacg gcgttccggc gcgcgcagcg tggatccagt
    12301 tcctgatcgt catcccgctg atgattatcc cgatgctcgg ttccaatacc gtgcaggatc
    12361 tgatgaatac aattattaat atgaccgccg cagcgtccat gcttccgccg ttattcatca
    12421 tgctggctta cctgaattta cgcgccaaat tagatcacct gccacgcgat ttccgtatgg
    12481 gctcccgccg caccggtatt atcgttgttt caatgctgat tgcgatattt gccgtagggt
    12541 ttgtcgcttc gacattcccg actggcgcga atattctgac catcattttt tataacgtcg
    12601 gc
//
LOCUS       DAJTMC010000123        12152 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.123, whole genome shotgun sequence.
ACCESSION   DAJTMC010000123 DAJTMC010000000
VERSION     DAJTMC010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12152
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.123"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(32..1741)
                     /locus_tag="OO130_003028"
     CDS             complement(32..1741)
                     /locus_tag="OO130_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCR5366347.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPKAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDAHLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTTLDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANAPLKPTVSGQLHAVNAAKQQVTQTMNAGIVSSGEVTSAE
                     LVQ"
     gene            complement(1862..3253)
                     /gene="xanP"
                     /locus_tag="OO130_003029"
     CDS             complement(1862..3253)
                     /gene="xanP"
                     /locus_tag="OO130_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HCR5366348.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKTWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITSTSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            3533..4738
                     /gene="gltS"
                     /locus_tag="OO130_003030"
     CDS             3533..4738
                     /gene="gltS"
                     /locus_tag="OO130_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HCR5366349.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            complement(4772..6853)
                     /gene="recG"
                     /locus_tag="OO130_003031"
     CDS             complement(4772..6853)
                     /gene="recG"
                     /locus_tag="OO130_003031"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HCR5366350.1"
                     /translation="MTGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDALKNKLLAALPFKPTGAQARV
                     VAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFEPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(6859..7548)
                     /gene="trmH"
                     /locus_tag="OO130_003032"
     CDS             complement(6859..7548)
                     /gene="trmH"
                     /locus_tag="OO130_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HCR5366351.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            complement(7555..9663)
                     /gene="spoT"
                     /locus_tag="OO130_003033"
     CDS             complement(7555..9663)
                     /gene="spoT"
                     /locus_tag="OO130_003033"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HCR5366352.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            complement(9682..9957)
                     /gene="rpoZ"
                     /locus_tag="OO130_003034"
     CDS             complement(9682..9957)
                     /gene="rpoZ"
                     /locus_tag="OO130_003034"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HCR5366353.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            complement(10012..10635)
                     /gene="gmk"
                     /locus_tag="OO130_003035"
     CDS             complement(10012..10635)
                     /gene="gmk"
                     /locus_tag="OO130_003035"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HCR5366354.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     DEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            10893..>12152
                     /gene="ligB"
                     /locus_tag="OO130_003036"
     CDS             10893..>12152
                     /gene="ligB"
                     /locus_tag="OO130_003036"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418104.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HCR5366355.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEIFRQQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQDDSDTLNSL
                     GVFVWAWPDGPQLMSDRLKELATAGFTLTQTYTRAVKNADEVARVRNEWWKAELPFVT
                     DGVVVRAAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     SVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVSL"
BASE COUNT         2709 a         3189 c         3327 g         2927 t
ORIGIN      
        1 acgtctgagt cgtacccggc gagattgatg tttactgcac cagctctgcc gacgtaactt
       61 caccgctgga aacgatgcca gcattcatgg tttgagtcac ttgctgcttt gcggcgttca
      121 cggcatgcaa ttgcccgcta accgtaggtt tcagcggggc attggcctga atatctccac
      181 tggcggtaag ctgaatattg ccgtcgccag tcaacggtaa ttcaggccat ccccattgtt
      241 gcaaaatatt caccggcaca ccgcgtccat tcaggctgat atgggtctga cgttgtggcg
      301 tttgtgaaac actggcagtg gcttccagaa tgcctttttc agtaaatgca ctcagttcgc
      361 tgatattcac cgtgctgctg ttggcggtca gcgccagcga cgggcgacga acatcaacac
      421 gattgaatgt cgcggcggcg gcattcagat tcgccgagcc actccagacg ccccatttat
      481 gatcggtaac cagcgtcagg ttggcaccgt aaccatcgag cgtggtgagc tgccacggga
      541 agtcagggtc gatatcaatg atcagattgc ggctggcgct aaatctcttc agttgcaggc
      601 tgtttaacca accgggtgtc gtttccatcc acaactgttg ccagtttttc ggcaaggtat
      661 attccagccc ggcaattgcc gcatcatcaa ggatcaacgt tttcccgtca cgcagccagt
      721 tccctgacgt tctgaccata cccccttccc agcggctggt gaactggcgc agcgctacgc
      781 cctgcgggga aaattccgcg tttataatcg ggtcaaataa atgcagcgaa ccataaatga
      841 actcgctagc gttcatcgac agtttgcctt cttgtgtctg ccagtcatct ttactgaagg
      901 tcatgttgcg caagctgaga tcgaggtcgg tcaccgccca gtccggacct tgcaaatgag
      961 catcgatcac ttccaggcga ccaatctgca acgacgggac agagcgtaat ggcgcaaaga
     1021 agtcggttag cgatttttcg ctttgtagac gtatatccgc catgcgcagg ttttccactt
     1081 gccagctgcc gtcggcgtta cgctgcgcgt ttccggttaa tgtcccgcgg gcgatgtcgg
     1141 cacccaggtt agtcagcgta acgcgatcgt tatcaatact gccttcaatc agtacattgg
     1201 tggcaggaac atcattgagc gaaagcgatc cggcactaaa ctgaatctgc gccttcgtac
     1261 ccagcacttt accggctttt ggtgaccacg gaactacgcc gccatttacc cgctgcgcgc
     1321 tcagtttcca ttcgctattc gggctattaa acgccatatc acgcagttgc agacgatcgg
     1381 ctttgaacgg tagcggcgcg gtctggtcgg tgagattcag cgtcccgttt tccagcagga
     1441 tggtatcgac atggcgtggt tcggtcagtt gccgactgct tagcgcaatg tcgacacttt
     1501 tggccaccag ggtcgcgggc tgaccatcac gaccaaacgt gacattctcc agcacgatat
     1561 gagatggcgc ggaaaaacgg tgatccatcg ccccgaaggc cagatgatag tcgctattct
     1621 cggaaaccca tgcgctgata tgttctgctc cccagcgggt ttgcagaaga aaatagaggc
     1681 cagcgatcac cactaacaga gcgatgagaa tgtagagaag cagcttccca ataaatttca
     1741 tggtcttcca ttccgtgaaa tgcacataag agagttatgc acgatttacg cgcaatcctc
     1801 aaggcgggaa tggtgaaaga ggtgagactg cggcggtaat tatcttaccg ccgccagtga
     1861 attactgttt ttctggtggg aaaatcagat tcagcacgat ggcagtaata ccgcccgcgg
     1921 cgatcccgga ggagagcagg tttttcagcc attcaggggc aaactgcaaa atcagcggct
     1981 gctgagacac gcccagacca accgccagcg acagcgcgat aatcagaatc gcccgacggt
     2041 tcagcggctc acgagaaacg atacgcacac cggaggcggc gatggtgcca aacattacaa
     2101 gcgttgcgcc gcccagaacc ggttctggaa tgtgttgtac aaaaccgctc actgccggga
     2161 acagacccag cacgatcaac atcagcgcga cgacaaaacc gacatagcgg ctggcaacac
     2221 cagtcaactg gatcactccg ttgttttgcc cgaagcagga gttcgggaag gtgttaaaca
     2281 ccgccgaaac aaacgagttc aggccgtttg ccagcacgcc gcctttcagg cgtttcatgt
     2341 acagcggacc ggacactggc tgttcggaaa cgtcagaggt cgacgtgata tcgccaatgg
     2401 tttccagcga agtgatcata aagaccagca tcagcggcag cagcagactc cattcaatgc
     2461 caagaccgta atagagcggc gttggcacca taatcagttc ttgcgtcatc ggttcgttgc
     2521 tttctggcaa catgcccata aaccacgcca gcgcatatcc ggccgccatc gcaatcacca
     2581 gtgaggccac gcgtaagtaa gggttacgtt gacggttaag caggataatt aaggctaaga
     2641 ccacgcctgc cagcagcaga tttttcggtg cgccgaaggt gttatcgctc atggctgcgt
     2701 aaccgccgcc aatggacgtt aacccaacct gaattagcga caggccgata atcatcacca
     2761 caacgccaga aaccagcggc gtaataatgc ggcgcgccag atgcagaacg cgggagatca
     2821 ccatctcggt gcaacttgcc agcatcaacg tgccgaacaa agccgccatc atggtaggaa
     2881 catcagcacc accggttttc agcgcggtac cgcccataat cagcggggca acaaagttga
     2941 agctggtgcc ctgaatagac aacagcccgg agccaaccgg accccaggtc ttaatttgaa
     3001 taatcgatgc cacaccggag gcaaacagcg acatactaat aatgtgttgc gtgtcttgtg
     3061 ccggtaaacc cagcgcctgg cagattaata gcgctggcgt gatcaccgca acgaacatcg
     3121 ccagcagatg ctgacaggcg gcaaacaggg tttgaggaag cggcggacga tcttcaagac
     3181 ggtaaatcag ttcgctgttt tgagtctgcg caaccggttg cgcattttct gactcgaggg
     3241 tggaaacaga catcgaaaac aatcccgtgg tagcaaagcg ggcattttag ctgaccgaaa
     3301 gagaaaagca aacgtttgcc acatcctcat caaaatattt aaatgagata aatgcacttt
     3361 tttatataac ttttggtatt tttctgctta aaatccatgc catttgttca ttaacagctg
     3421 agaatttgac ccaggaaagc ataactcgat acccgtcata gttcatgaag ttgccactgc
     3481 gcgatgaagt atgacgagta tgaaagagtg atgcggatac aaaggagtaa ctatgtttca
     3541 tctcgatact ttagcaacgc ttgttgccgc aacgctgacg ttgctgctcg ggcgtaagtt
     3601 ggtccattcc gtctcctttt tgaagaaata caccataccg gaacctgttg cgggtggttt
     3661 gttggtggcg ctggcgctac tagtactgaa aaaaagcatg ggctgggaag tcaactttga
     3721 tatgtccctg cgcgatccgt taatgctggc tttcttcgcc accattggcc tgaacgccaa
     3781 cattgccagt ttgcgtgccg gtgggcgtgt ggttggcatc ttcttgattg tggttgttgg
     3841 tctgttggtg atgcaaaatg ccattggcat tggtatggct agcctgttag ggcttgatcc
     3901 gctgatgggg ctgttggccg gttctattac gctttccggc ggtcacggta cgggcgctgc
     3961 gtggagtaaa ttgttcattg aacgttatgg cttcaccaat gcgacggaag tggcgatggc
     4021 ctgtgcaacg ttcggtctgg tgctgggcgg cttgattggc ggtccggtgg cgcgctatct
     4081 ggtgaaacac tccaccacgc cgaacggtat tccggatgac caggaagtcc cgacggcgtt
     4141 tgaaaagccg gatgtgggac gcatgatcac ctcgttggtg ctgattgaaa ctatcgcgct
     4201 gattgctatc tgcctgacgg tggggaaaat tgttgcgcaa cttttggctg gcactgcttt
     4261 tgaactgccg accttcgtct gtgtactgtt tgttggcgtg attctgagca acggtctgtc
     4321 aataatgggc ttttaccgcg tctttgagcg tgcggtatcc gtgctgggta acgtaagctt
     4381 gtcgttgttc ctggcgatgg cgttgatggg gctgaaactg tgggagctgg cttcgctggc
     4441 gctgccgatg ctggcgattc tggtggtaca gaccatcttc atggcgttgt atgccatctt
     4501 cgttacctgg cgcatgatgg gcaaaaacta cgatgcggca gtgctggctg cgggtcactg
     4561 tggttttggc ctcggtgcaa cgccaacggc aatcgccaac atgcaggcga tcactgaacg
     4621 ctttggcccg tcgcacatgg cgtttttggt ggtgccgatg gtcggtgcgt tctttatcga
     4681 tatcgtcaat gcgctggtaa ttaagttgta tttgatgttg ccgatttttg ccggttaacc
     4741 gatgaagcgg cggtcttctc accgccgcct tttacgcatt cgagtaacgt tctgtctccg
     4801 gcatccagcg ttctatcaag gcttttgcct gttgtgggta acgttcgtga atatgtcgcg
     4861 ccaggcgctg aacttccggg atcatcgcct gatcgcgcag taaatccgcc actttaaatt
     4921 cagcattacc cgtctgacgc gtgcctaaca attcgccagg gccgcgaatc tccagatctt
     4981 tttgcgcaat cacaaaaccg tcgttactgt cgcgcagcac ttgcaggcga atttgcgccg
     5041 ttttagaaag cggcgttttg tagagcagca cgcagtgaga agccaccgcg ccacgaccta
     5101 cgcgcccgcg cagctggtgt aactgcgcca gacccagacg ctcagggttt tcgataatca
     5161 tcaggctggc gttaggcaca tcaacaccga cttcaataac ggtagtggca accagcagat
     5221 gtagctcacc ttgtttaaac gacgccatca ccgcctgttt ctcggctggt ttcatccgcc
     5281 cgtgtaccag gccaacattc aattctggta gcgccagttt caactcttcc caggtagctt
     5341 ccgccgcctg cgcttccagt aactctgact cttcaatcaa cgtacaaacc cagtacgcct
     5401 gacgaccttc agttatgcag gcatggcgca cgcggtcaat gatgtcggta cggcgggtat
     5461 caggaatagc gaccgtagtc actggcgtgc ggcctggcgg cagctcatct atcaccgagg
     5521 tatcgagatc ggcatacgcc gtcattgcca gcgtgcgggg gatcggcgtg gcggtcatga
     5581 tcaactgatg cggatggaag ccttgctgct ggcctttctc ccataacgcc agacgctgat
     5641 gcacgccaaa gcgatgttgt tcgtcgataa tcaccagcgc cagaccgtta aactgcacct
     5701 gttcctggaa gatggcgtgc gtaccaacaa tcatctgcac ctgaccgctg gcgatggctt
     5761 cctgctgaga cagtcgcgct ttgcctttct gcttaccggc gagccagccc acttcgatac
     5821 cgagcggttc aaaccagttg cggaagttat tggcgtgctg ttcggcgagt aattcggttg
     5881 gtgccatcaa tgccacctgt ttgccgtggg caatcgcacg cagcgcggcg agggcggcga
     5941 ccagcgtttt accggaacct acatcgccct gtaccaggcg catcatcggc acatccagcg
     6001 ccatatcgcg ctcgatctcc gccactacgc gtgcctgtgc gcccgttggc ttgaacggta
     6061 aggctgcgag gagtttattt ttcagcgcat cattggcgct cagcggctgg gcatgaaaac
     6121 gctgtgctcc ggcacgtaag gctaacatgc tgaggttgtg cgccagcagt tcttccagaa
     6181 tcagacgacg ttgcgccgga tgctgcccgg tttccagatc gctaagctgt agcgtcggtg
     6241 gcgggcggtg caaagtgcgc aacgcttccg gtagcgtcat cattccttgt gacagttccg
     6301 gcggcaggag ttcttcaatg gcgcaggtgt cgagcagatc cagcgcctgg tcggttaatt
     6361 tacgcagcgt ggcctgcttt acgccttccg ttgttggata aaccggcgtg agcgtttcct
     6421 gtaattctgg cgtgctgaga tcgccctgca cgcggtattc cgggtggatc atctccgcac
     6481 catatttacc gcgctttgct tcgccataag ccagtacacg gcggcccgtc gccaggctat
     6541 ttttcattgc cgcgctgaaa ttgaaaaagc gcatggtgag gatgccggaa ccgtcgctga
     6601 tctggcaggt catcatccgc cgaccgccga aggagatatt gcagttcagc acttcgcctt
     6661 ccaccgtggc ataaacgccc ggcagtagtt ctccgatggg gtagagatgg gtgcgatctt
     6721 cgtagcgcag gggaaggtgt aagagtagat cctgcacggt atgcaggttg attttcgcca
     6781 gtttgttact aagtgctgcg ccaacgcccg ttagggaact gagtgggaca gcatctaaca
     6841 ggcgacctgt catggcactt accctgcagc ctgcatagta gcccaccagt cggcatcagc
     6901 ttcgatctcg ccttgctgat tgacgtgggg ataaggcagg cctttgcgtt ttgcgacttt
     6961 cgccagcacc ggatagccgc cttcaaacaa caggcgttgt tgctctgctt ccggcaacat
     7021 gctgttttca cgcaggtaca tgcctgcatt ttgccgctga cgctgggctt cgtaaagaat
     7081 gagggctgag gcaacggaaa cattcagcga ctgcaccatg ccgatcatcg gaatgatgat
     7141 gtcctgatcc gccagggcca atgcttcctg cgtgatgccc gttttctcct gtcccatcaa
     7201 aatgcaggtc gggcgagtgt aatcaatttc gcggaaatcg acagcgttat cagaaagatg
     7261 ggttgccaga atctgcatgc cctggccttt gagatgagcg acggcatcgc caatggtgcg
     7321 gtgtgttttc acctgtaccc agctgttact acccgccgct gccgaagcca tggtgcgcat
     7381 gcggctacca ggccagacgg cgtgaacttc atgtacgcca acggcatctg cggtacgaat
     7441 aatcgcagaa acgttatgag gtttgtggac ctgctccatg cagacggtca gatcaggctg
     7501 ccgcctggcg agcatttcgc agatgcgtgc ataacgtgtt gggttcataa aacattaatt
     7561 tcggtttcgg gtgactttaa tcacgtctgg catcacgcgg attttgcgca tgatattcgc
     7621 cagatgcaca cggtcacgag cggtcagacg aataaaggcg ctgtagacgc gaccatcttt
     7681 ctcttccgta ttcaaacttt gaatattcga agtcgtggtg ttaattgccg ccgtcaggtt
     7741 tgccagcgca ccctgatgat tgaacatctc caccttgatt tcggtgatga actcctgcgc
     7801 cgtctcttta tcccattcca cagccataaa cttctctggc tctttctggt agccacggat
     7861 gttacggcag gattcatggt ggatcaccag acctttaccg gggctgacgt gggcgataat
     7921 cgggtcgcca ggaatagggc ggcagcattt cgcaaaggtg atcagcacgc catccgcgcc
     7981 tttgatcggc agatgcccgt ggctttgagt cgccggtgga atggaggcgt ccccatgttg
     8041 tagatttttc gcgaccacca cgctcattgc gttaccaagg ccgatttctg ccagcagatc
     8101 gtcaagcgtt gccagcttca tgcgatccag ctcgcgctga atattttcct gcgggatttc
     8161 attgagctta cggctaccac ccaaagcatg gttgagcaga cgacggccca ggcttacaga
     8221 atcatcacgc ttgaggtttt tcagcaactg acgaattttg gcgcgcgctt tcgagctaac
     8281 gacaaagttc agccaagcgg cattcgggcg agcgcccgga gcggtaatga tttcaacggt
     8341 ctgaccgcta gtaagcggct gcgacagcgg gtaaggctgg cggtcgacgc gtgcgcccac
     8401 gcaggcatgg ccgatatcgg tatgcactgc ataagcgaag tcgacgggcg ttgcaccggc
     8461 aggcagctcg acaatgcgcc cttccggtgt gaaaacgtaa atctcatccg ggaagagatc
     8521 ggatttaacg ctctcgataa attcaaacga actaccggcg ctctgttgca gctccagcag
     8581 gctttgcatc cagcgctggg cgcgtatttg tgcggtagta ctggtttcgc cgtgctcttt
     8641 ataagcccag tgcgcggcaa cacccatctc cgccatctga tccatatctt cggtacggat
     8701 ctggacctca accggcacgc cgtgcgggcc aatcatcgag gtgtgcaacg actgatagcc
     8761 gttcgctttt ggaatggcga tatagtcttt cacgcggccc ggacgcggct tgtacaggct
     8821 gtgcatctgg cccagcacgc gataacaggt gtcagaatca ttgacgatca cgcggaaagc
     8881 gtagatgtcc atgattgagt gaaaacgctg ctctttgagc accattttgc agtaaatcga
     8941 ataaagatgc ttttcgcgac cactgacgcg gcacggtatt cccgcttcct gcaaacgccc
     9001 ttcgatttca gaaagaatct tctggatcat ctctttacgg ttgccgcgcg cggctttcac
     9061 cacttctttg attacgcgat aacggttggg atacagcgcc tcaaaaccca gctcttcgag
     9121 ttcggtttta atgtggtgga tacctaaacg gtgcgccagc gggctgtaaa tttcgagagt
     9181 ttcacgggcg atgcggcgac gtttgtccgg gcgaagtgag cccagcgtgc gcatgttgtg
     9241 ggtacggtcg gcaagtttga tgaggatgac gcggatatcc tgcaccatcg ccataatcat
     9301 cttgcgaaag ttttcggcct gcgcctcttt cttatcgcgg aacttgagtt tatcaagttt
     9361 cgacaccccc tctaccagct cggcgacgct tttaccaaaa agctgttcca tatcctggta
     9421 ggtggcggga gtatcttcaa tcacgtcatg cagcagcgcc gccatcagcg tttcatagtc
     9481 gagtttcatc tcggccagaa tgcaggcaac cgctaccggg tgcgtgatat agggttcacc
     9541 gcttgaacgt gtttgcccct cgtgagcatc acgtgcaacg agatacgcct gccgcagacg
     9601 cttgatttgg tcttccggca ggtaggtttg aatcagttga ttcaggcttt caaacagata
     9661 caagggcgac ccgctttgtg attaacgacg accttcagca atagcggtaa cggcttgtaa
     9721 ttcagcggct tcctgctctt gctgttcctg gcgttcgcga acgtcgagga tctggttgtt
     9781 gatcagacct tcttcgattt cgcgcagcgc gattacagtg gttttatcgt tttcttccgg
     9841 taccagcgga tcctttccgc ctacctgcat ctgacgagcg cgacgcgcgg cgaccagtac
     9901 caggtcaaaa cggttaccaa ttttctctac agcgtcctga acagttacgc gtgccatact
     9961 taaaaagctc cacaggtgaa gaaatgactg ggcatgatac tgaaatcagg ttcagtctgc
    10021 caacaatttg ctgattaaag cgtcatgacg ctgcttttgg cggctcatgc gcagacgttc
    10081 ggcgcgaata atggtcttca aatcggtcaa cgcggtatcg aagtcatcat tcacaatcag
    10141 gtaatcatat tcgtcgtaat ggctcatttc tgcaacagct tgcgccatac gctttgcaat
    10201 gacctcttcg ctgtcctgac cgcgaccgcg tagacggcgg tccagttcaa ttttagacgg
    10261 cggtaaaata aagatactcc gcgcgtgcgg catcttctgg cgaatttgct gcgcgccctg
    10321 ccagtcgata tcgagaaaaa catcgacacc ggtcgccagt acttgctcaa tggcctcacg
    10381 cgaagtgcca tagtaattac caaaaacttc tgcgtgttcg aggaacgcat ctctgctaat
    10441 catttcttta aattcatcat gattaacaaa gaaataatgt tcaccgtgga cttcaccagg
    10501 acgcggttgg cgtgtggtgt gtgaaacaga aacctgggtg tcatacaacg gttgggtttt
    10561 taataaagcc tgaatcaggc tggatttacc cgcgccactg ggggcagaaa caatataaag
    10621 cgtgccttga gccatgagta tctttcgtat gtgattagcg aaataaagcc tacatacgag
    10681 cttattatac acggcgcggc catgtgacgt agcccttgct cacacttttt gcgcaaagtt
    10741 gttgcgtttt gcgtagctgc ttccgcattt gcctgtgatt tactgcaata aagacgattc
    10801 tcaggaagcg tcctcgcaga ctccgttttt gccactcggc tttgccttca ccacgttgta
    10861 tatctgccag cctgactaca gcggaggtga agatgaaagt atggatggcg atattaataa
    10921 gtatcttgtg ctggcaatca tctgtgtggg cggtctgtcc ggcctggtcg ccagccagag
    10981 cacaggaaga aattttccgc cagcaacagc aaataaaaca gtgggacgat gactactgga
    11041 aggaaggaaa aagtgaggtg gaagacggtg tttacgatca gttaagcgcc cgtcttacgc
    11101 agtggcaacg ctgctttgtg agcgagcccc gcgacgtgat gatgccgcct ttaaatggtg
    11161 cggttatgca tccggttgcc catacgggtg tacgcaaaat ggcggataaa aacgcattaa
    11221 gtctgtggat gcgagaacgt agcgatcttt gggtgcagcc aaaagttgat ggcgttgcgg
    11281 taaccctggt ttatcgggac gggaaactga acaaagcaat cagtcgcggt aatggcctga
    11341 aaggcgagga ctggacgcag aaagttagct taatttccgc tgtgccgcaa accgttagcg
    11401 ggcctttagc caacagtacg cttcaggggg aaatatttct ccagcgcgag gggcatatcc
    11461 aacaacaaat ggggggaata aatgcccgcg caaaagttgc tggcttgatg atgcgccagg
    11521 acgatagcga cacgctgaat tctctgggcg tttttgtctg ggcatggccg gatggaccgc
    11581 agttaatgtc tgatcgttta aaagagctgg ctaccgcagg ttttactcta acgcagacgt
    11641 atacccgtgc ggtgaaaaat gctgatgaag ttgcgcgcgt acgcaatgag tggtggaaag
    11701 cggaattacc cttcgtcacc gatggcgtag ttgtacgagc ggcgaaagag ccagaatccc
    11761 gccattggct accgggccag gcagagtggc tggtggcctg gaaatatcaa cctgtagctc
    11821 aggttgccga agtgaaggcg attcagtttg cggtgggtaa gagcggtaaa atatcggtgg
    11881 ttgcgtcact cgcatctgtc atgctggatg ataaaaaagt ccagcgggtg aatattggtt
    11941 ccgtcaggcg ctggcaggag tgggatattg cgcctggtga tcagattctc gtcagccttg
    12001 ccggtcaggg tattcctcgc attgatgatg tggtgtggcg cggtgcagaa cgtacaaaac
    12061 cgacaccgcc agaaaaccgc tttaactcgt tgacctgcta ctttgcttct gatgtttgtc
    12121 aggaacagtt catttcacgc ttagtcagtc tg
//
LOCUS       DAJTMC010000124        12075 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.124, whole genome shotgun sequence.
ACCESSION   DAJTMC010000124 DAJTMC010000000
VERSION     DAJTMC010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12075
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.124"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            <1..192
                     /locus_tag="OO130_003037"
     CDS             <1..192
                     /locus_tag="OO130_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCR5366356.1"
                     /translation="RGAFFISALFFFNAVCIVSDNLLIIESFGEMAYNISYLTRVPGT
                     NTLLACCCLLRPEEVNSEY"
     gene            182..355
                     /locus_tag="OO130_003038"
     CDS             182..355
                     /locus_tag="OO130_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HCR5366357.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(404..1476)
                     /locus_tag="OO130_003039"
                     /pseudo
     CDS             complement(404..1476)
                     /locus_tag="OO130_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415512.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
     gene            complement(1548..4292)
                     /gene="torS"
                     /locus_tag="OO130_003040"
     CDS             complement(1548..4292)
                     /gene="torS"
                     /locus_tag="OO130_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HCR5366358.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFAAQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     GFDTTAIEQQEQEISRSLRQQGELVGRRLQLRQQQQQLSQQIVAAADEIARLAQGQAN
                     NATTSAGATQAGIYDLIEQDQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPMLEKQLNNAVKILQRRQIRIEDPGVRAQVATTLTTVSQYSDLLALY
                     QQDSEISNHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHLEKASARGQYSLL
                     LLGMVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     RLMDAFRSTVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATAIADDVPTALMGDPRRIRQVI
                     TNLLSNALRFTDEGQIVLRSRTDGEQWLVEVEDSGCGIDPAKLAEIFQPFVQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTPEVGSCFCLRLPLRVATAPVPKTVNQAVRLDGL
                     RLLLIEDNPLTQRITVEMLNTSGAQVVAVGNAAQALETLQNSEPFAAALVDFDLPDVD
                     GITLARQLAQQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYL
                     QLQVNNDQSLDVSQLNEDAQLMGTEKIHEWLVLFTQHALPLLDEIDIARASQDSEKIK
                     RAAHQLKSSCSSLGMRIASQLCAQLEQQPLSAPLPHEEITRSVAALEAWLHKKDLNAI
                     "
     gene            4375..5364
                     /gene="torT"
                     /locus_tag="OO130_003041"
     CDS             4375..5364
                     /gene="torT"
                     /locus_tag="OO130_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HCR5366359.1"
                     /translation="MRVLLFLLLSLFMLSAFSADNLLRWHDAQHYTVQASTPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAGGYSQLATQQAQIDQCKQWGAEAILLGSSTTSF
                     PDLQKQVANLPVIELVNALDDPLVKSRVGVPWFQMGYQPGRYLVQWAHGKPLNVLLMP
                     GPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQEMLERHPEIDVVAG
                     TAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASDQMIWQGELAVEQA
                     IRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFYQHTSAAKK"
     gene            complement(5337..6029)
                     /gene="torR"
                     /locus_tag="OO130_003042"
     CDS             complement(5337..6029)
                     /gene="torR"
                     /locus_tag="OO130_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HCR5366360.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQT
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGECYFLAADVC"
     gene            6149..7321
                     /gene="torC"
                     /locus_tag="OO130_003043"
     CDS             6149..7321
                     /gene="torC"
                     /locus_tag="OO130_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HCR5366361.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHAKQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEISHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDIAGKAHGDKKEEK"
     gene            7321..9917
                     /gene="torA"
                     /locus_tag="OO130_003044"
                     /pseudo
     CDS             7321..9917
                     /gene="torA"
                     /locus_tag="OO130_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
     gene            9914..10513
                     /gene="torD"
                     /locus_tag="OO130_003045"
     CDS             9914..10513
                     /gene="torD"
                     /locus_tag="OO130_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HCR5366362.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLAAAVNELENCIATLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQ
                     EIKRLLVEAGMETSGNFNEPADHLAIYLELLSHLHFSLGEGTVPARRIDSLRQKTLTA
                     LWQWLPEFAARCHQYDSFGFYAALSQLLLVLVECDHQNK"
     gene            complement(10606..10911)
                     /gene="cbpM"
                     /locus_tag="OO130_003046"
     CDS             complement(10606..10911)
                     /gene="cbpM"
                     /locus_tag="OO130_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HCR5366363.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(10911..11831)
                     /gene="cbpA"
                     /locus_tag="OO130_003047"
     CDS             complement(10911..11831)
                     /gene="cbpA"
                     /locus_tag="OO130_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HCR5366364.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGAKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
BASE COUNT         2897 a         3100 c         3200 g         2878 t
ORIGIN      
        1 cgtggagcct tttttatatc tgccttattt ttcttcaacg ctgtatgtat agtaagcgat
       61 aacctgttga ttattgaatc tttcggggag atggcttata acatttctta cctgaccagg
      121 gtaccgggaa ccaacacctt actggcgtgt tgctgtcttt taagaccaga agaggttaac
      181 agtgaatatt gaagagttaa aaaaacaagc cgaaacggaa atcgccgact ttatcgcgca
      241 aaaaatcgcc gagctgaaca agaatacagg gaaagaagtc tctgaaattc gcttcaccgc
      301 acgagaaaaa atgaccgggc ttgaaagtta tgatgtcaaa atcaaaataa tgtgattttg
      361 tgaacatcac cccgtgcgag gtgatgttcc gcttgttgct aatttagtga tcaatcattg
      421 gcgcttgtgg aattaagcgt cggtacaatt cctccggcac cgggctttgc catactcccg
      481 catacattgc gtaaccaatc accgcgaaca taatccccag aaccagtagc gtcattaacc
      541 agccagacaa cgcaaaagct tttttatttg ccgcaggttt ttgcagtgaa aaggtcaatg
      601 ttgaggctac cggacatgac tctacgcaag tcatacagcc ggtacattcc actgttcgta
      661 cctgaattaa tttatctacc gggatccgtg atgggcaatt ttttgcgcat ttgccacagc
      721 cgatacaact ttcggcattg cgacgaatct taaacggcga taatagcgaa accacgccca
      781 tcagcgcgcc atatgggcaa agataacgac accaggcatg gcgaataaac aggctggcaa
      841 tcagcaaaac ggtcacgctg attaatgtcg cggtccccat atgacgaaag aaatcgagca
      901 tttttacgtc catcaccacg ctgtagggcg acaacataaa atagtgaatc gcctgagcgg
      961 gcatcaataa cgcgatatag agaaaaaaac tcaacagcaa atacttcacg ccgcgcagag
     1021 gaatatccag ccagcgggga aggacacatt gccgaccaaa cagtttgtta ccgagatcgc
     1081 cgattaattc agaaagcgta ccaaccgggc ataaccatga gcaaaaggcc tttttgagta
     1141 atagactgat gacgataaaa gcgaccaata acagcatcgc ggagcgtgga cggacggtaa
     1201 ctgacctgtt gtaaggctat atttcagatt catcagaccg gcaatcggta gccagccttc
     1261 gatacctccc ggtctggcga caaatgtcgt gctacttgcc gtttcgtaat agcgcaccca
     1321 ataccagaac gtgatggcaa tataaatatt cattgccaac agtaataatt gcgtcgcttt
     1381 acgccaggtc gtggcattgc gccagtcatt ccacggtaat ttgccgcccg tcgtgcctgg
     1441 ccgccgctgc cagcgggttc tttttttctc tgccatgatt ttgccagtcc gttaagttgc
     1501 ataccaaatg ccactatttt agttgttctt aactggctga tattgattca aatcgcgttc
     1561 aggtctttct tatgcaacca tgcttccaga gcggcaacac tgcgtgtaat ttcttcgtgt
     1621 ggaagggggg cagataatgg ctgctgctcc agttgtgcgc atagctggct ggcgatacgc
     1681 attcccaaac tcgagcaact gctttttagc tgatgcgcgg cacgctttat tttctcgcta
     1741 tcctgactgg cgcgggcaat gtcgatttca tcgagaagcg gcagggcatg ttgtgtaaat
     1801 aataccagcc attcgtggat cttctccgtc cccattaact gagcatcttc attgagttgc
     1861 gatacatcca gcgattgatc gttattgact tgcagttgga gatagtgcgc cagtaactga
     1921 ccgagcactt cacgcggcac cggtttaggg ataatcccgc ggaataatga actggtacgc
     1981 tggcgcagcg tttcgtcaat gacatgggcg ctaaagccaa tcaaaaccag cgacggatat
     2041 tgctgtgcca gttgtcgggc aagcgtaatg ccgtcgacat ccggcagatc aaaatccacc
     2101 agtgcggcag caaacggttc gctattttgc agtgtctcta aagcctgcgc ggcattgcca
     2161 acagcaacaa cctgcgcacc actggtgttc agcatctcaa cggtaattcg ctgggttagc
     2221 gggttatctt caattaacag caaacgtaaa ccgtcaagac gcaccgcctg attgactgtt
     2281 tttggcacgg gtgccgtggc aacacgtaac ggcaagcgta aacaaaaaca gcttccaacc
     2341 tccggcgtgc tggtggcgct cagttcgccg cccatcgcct gggccagacg gctactgata
     2401 gtcagtccca gcccggtgcc gccgcgtttg ccgcttacct gcacaaatgg ctggaagatt
     2461 tctgccagtt tcgcgggatc aataccgcag ccgctgtctt ccacttcgac cagccattgc
     2521 tcgccatcag tgcggctacg taaaacgatc tgcccttcgt cagtaaaacg cagggcgttg
     2581 ctcaacaggt tggttataac ctgacgaata cgtcgcggat cgcccattaa cgcggtcggt
     2641 acatcgtcgg caattgctgt tgccaggcga atcgggcgac ctttcacccg tccgctcatt
     2701 aattgcaggg tactttccag cagcgggcgc ggttcaaagg gctcatcgct gaccgaaaca
     2761 ttcttgccac ctgcttcgat agccgaataa tcgagaatat cgttgaggat ggtcagcaac
     2821 gattcgccag agtcggtaat cgcccgcaaa tcatcacgct gggcgttaag tgcggggtta
     2881 tctgccagca gttgagcagt gccgagaata ccgtacagcg gtgtgcggat ctcatggctc
     2941 atcgccgcca gaaaggccga ttttgcctgg ctggcttttt ctgcttccgc ccgtgcctgt
     3001 cggtgttcta tcaccagttc ctgcaattca gctgtacgcg ctttgacctg cgccgccagc
     3061 tgttcacggt ggcgattcag tgcatgaaca gtgctgcgaa acgcatccat cagccgcccg
     3121 atggtatcca gctcccgtac gccagcggtt tccgggaaag gggagtcaat atcaccgtcc
     3181 agcagccgtt gcagcgcctg cgtttgttcg gcaagtggac gcgtgactga gcgataaacc
     3241 acgcgccaaa ggatcagaat cagtgcgcaa agtgaaacca tccccagcaa taacaggctg
     3301 tattgcccgc gtgcactggc tttttccaga tgcgccagtc cgtgctgatt acgcagctca
     3361 atggtgtcga ccagctgact gacttcgcta ctaaactgcg cgaactgggc gatgttattt
     3421 tgtgcgagag tttgtaggtg attgctgatt tcactgtcct gctgatacag cgccagcaaa
     3481 tcgctatatt ggctaacggt agttaacgtt gttgcgacct gcgcacgaac acccggatct
     3541 tcaatgcgta tttgccgacg ttgcagaatt ttcaccgcat tattgagctg cttttccagc
     3601 attggtgcat ttttctggat ctgctccagc cccagattca tcaccatttg ctgcacccgc
     3661 agagcgctaa ggcgcagttc attcatctgg ttaacatact caagatcgat atcaatcagc
     3721 cgatcgagtg cactttcagc agcctgacgc tgatcttgtt cgatcaaatc gtaaatcccg
     3781 gcctgggtcg ctccagcgga agttgtcgca ttattcgcct gaccttgcgc caggcgtgcg
     3841 atctcatcgg cggcagcgac tatctgctga ctgagttgct gttgttgctg gcgtagttgc
     3901 agacgccgcc ccaccagttc cccttgctga cgtaacgaac gggagatctc ctgctcctgt
     3961 tgttcaatag cggtggtatc aaaaccttgt tcccgtaacg cttgcagcaa cgcattaatc
     4021 ttcaggcttt gtgcggtgag cattcgcccc tgcgcctgcc acatcttttc gttatcggca
     4081 ctggtcaggt tctgcgcggc gaaaagttcc caggcgctgg cttcgctcaa ctggcgcgcc
     4141 atattcatgg taggaatcaa tgcctgagtg ttgtcttttt ccacctggct gataaagcgc
     4201 aggttgtacc atcccaccag ggtactggtc agggttaaca gcgccatcag ggcaaagccc
     4261 atccagagtc ttcgggtcag ggttaaattc acggtcggtg cactttaggt gaaaaaggtt
     4321 gagtcgcaaa gcagaatgca tctagcataa agccttatta ttgattaggc tatcatgcgc
     4381 gtactgctat ttttacttct ttcccttttc atgttatcgg cattttcggc tgataacctg
     4441 ttgcgctggc atgatgcgca gcattacacg gtgcaagcct ctacgccgct taaagccaaa
     4501 cgcgcatgga aactgtgcgc gctttatccc agcctgaaag attcatactg gttatcgttg
     4561 aactatggta tgcaggaggc aggcggctac agccagttgg ctacccagca agcacaaatc
     4621 gaccagtgta aacagtgggg cgcagaggcc attttgctcg gtagtagcac tacgtcattt
     4681 cccgacctgc aaaagcaggt agcaaatctg ccggtgatcg aactggtaaa tgctcttgat
     4741 gatcccctgg tgaaaagccg cgttggtgta ccctggtttc agatgggcta tcaacctggg
     4801 cgatatttgg tgcaatgggc gcacggtaaa ccactgaacg tgctgttgat gcccggaccc
     4861 gataatgccg ggggcagtaa agagatggtc gagggttttc gcgcagccat tgccggaagc
     4921 ccggtgcgta ttgttgatat tgcgcttggt gataacgata ttgaaatcca gcgtaacctg
     4981 ttgcaggaga tgctggaacg ccacccagaa atcgacgtcg ttgccggaac agccattgcg
     5041 gcagaggcgg caatgggtga agggcgtaac ctgaaaacgc cgcttaccgt ggtgtcgttt
     5101 tatctttcac atcaggtgta tcgcgggctg aagcggggaa gagtgattat ggctgccagc
     5161 gatcaaatga tctggcaggg ggaactggcg gttgagcagg ccatcaggca attacagggg
     5221 caatcggtgt ctgataatgt cagcccaccg attttagttc tgacgccgaa aaatgccgac
     5281 cgtgaacata ttcgccgctc gctgtcacca gggggatttc gtccggtcta tttttatcag
     5341 cacacatcag cggctaagaa ataacattca ccatgttgcg tcaccagtaa atccgcgctg
     5401 agtttatgac gtaaacgacg aattaacaca tcgacggtgc gcaggtcagg gttttccacc
     5461 cgacgcgcag aaagcatacg tagcagacgt tcacggctga gaatttcgcc cggattcgtc
     5521 acaaatgcca ccaacatttc atactctgcg cgggtcagtt taatcggctc gccatcccgc
     5581 tccagcgtat ggcgcgacac attcaggcaa taaccggcaa agcgatagca gttgtcctga
     5641 gtgtgcggtt gagcttgtcg cgcgaggtcg attcgccaga gcagattttt cacccgtact
     5701 accagttcgc gcagttccag cggtttggtg acgtaatcgt ctgcgcccat ttccagccca
     5761 acaatacggt caatccgatc gctgcgtccg gtaaccagaa taatccccac cgttgagcgt
     5821 tctcgcaggg cgcgggttaa catcaggcca ttttcatcgg gtaagttgat atccagcaga
     5881 attaaatcta ccggctgagt ctgcataatt tcccgcagcc cggcaccgct cgctgtaacg
     5941 gaaacggtat acccctcctg agtgaagtaa gattgtaatc gcgcctgggt aaccggctca
     6001 tcttcaacaa taacaatgtg atgtggcatc agagggtttt actcattctg ttcatattct
     6061 gccgtaagcc gttcatcctg accagtgccg ctgttcatat ttgctcatta agatcgcttc
     6121 actaaaccat aattttacag gggctattat gcggaaactc tggaacgcgc tacgccgacc
     6181 cagtgctcgt tggtcggtac tggcgctggt cgcaattggg attgtgattg gcattgcgct
     6241 gattgtattg ccacacgttg ggatcaaagt caccagcaca accgaatttt gtgtcagttg
     6301 ccacagtatg caaccggtgt atgaagaata taaacagtcg gtgcatttcc agaacgcctc
     6361 cggcgtgcga gctgaatgcc atgactgtca tatcccgccg gatattccag gcatggtgaa
     6421 gcgcaaactg gaagcgagca atgatatcta ccagaccttt attgctcact ccattgatac
     6481 acctgaaaaa ttcgaagcca aacgcgcgga acttgccgag cgtgaatggg cgcgaatgaa
     6541 agaaaacaac tcggcaacct gccgctcctg ccataactac gatgcgatgg atcatgcgaa
     6601 gcagcatcct gaagcggcac gtcagatgaa ggtggcagcg aaagataatc aatcctgcat
     6661 cgactgtcat aaaggtattg cccaccagtt accggatatg agtagcggct tccgtaagca
     6721 gttcgatgag ctgcgcgcca gtgctaatga cagtggtgac acgctgtact ctattgatat
     6781 taagccaatt tatgcggcga aaggcgataa agaagcctct ggttctctgc tgcctgcttc
     6841 ggaagtgaaa gtccttaaac gtgacggcga ctggctgcaa attgaaatta ccggctggac
     6901 ggaaagcgcc ggacgtcagc gtgtactcac ccaattccca ggtaaacgca tctttgttgc
     6961 ctcgattcgt ggtgatgtgc agcagcaggt aaaaacgctg gagaaaacca ccgttgccga
     7021 caccaatacc gagtggagca agttgcaggc cactgcgtgg atgaagaaag gtgatatggt
     7081 gaacgatatc aaaccgatct gggcttatgc ggattcgttg tacaacggca cctgtaacca
     7141 gtgccacggc gcaccggaaa tctctcactt tgatgctaac ggctggattg gcacgctcaa
     7201 cggcatgatt ggctttacca gtctcgataa acgtgaagaa cgcaccttgt tgaaatatct
     7261 gcaaatgaat gcgtctgaca tcgcaggtaa ggctcacggc gataagaagg aagaaaaata
     7321 atgaacaata acgatctctt tcaggcatca cgtcggcgtt ttctggcaca actcggcggc
     7381 ttaacagtcg ccgggatgct ggggccgtca ttgttaacgc cgcgcagtgc gactgcggcg
     7441 caagcggcgg atgtcatctc gaaagagggc attcttaccg ggtcgcactg gggggctatc
     7501 cgcgcgacgg tgaaggatgg tcgctttgtg gcggcaaaac cgttcgaact ggataaatat
     7561 ccgtcgaaaa tgattgccgg attgccggat tgccggattg ccggattgcc ggattgccgg
     7621 attgccggat tgccggattg ccggatcacg tacacaacgc ggcgcgtatt cgttatccga
     7681 tggtacgcgt ggactggctg cgtaagcgcc atctcagcga tacctcccag cgcggtgata
     7741 accgttttgt gcgcgtgagc tgggatgaag ccctcgacat gttctatgaa gaactggaac
     7801 gcgtgcagaa aactcacggg ccgagtgcct tgctgaccgc cagtggttgg caatcgacgg
     7861 ggatgttcca taacgcttcg gggatgctgg cgaaagctat tgccttgcat ggtaatagcg
     7921 ttggtacggg cggagattac tctaccggtg ctgcgcaggt gatcctgccg cgcgtagtcg
     7981 gttcgatgga agtgtatgaa cagcaaacct cctggctgct ggtattgcag aacagcaaaa
     8041 ccattatgct gtggggctcc gatttgctga aaaaccagca agcgaactgg tggtgcccgg
     8101 atcacgatgt ttatgaatat tacgagcaac taaaagcgaa agtcgccgcc ggtgaaattg
     8161 aggtcatcag catcgatccg gttgtcacat ccacccatga gtatctgggg cgcgagcatg
     8221 tgaagcacat tgcggttaac ccgcaaactg acgtgccgct gcaactggcg ctggcgtata
     8281 cgctgtacag tgaaaacctg tacgacaaaa actttctcgc taactactgt gtaggttttg
     8341 agcagttcct gccgtatctg ctgggtgaga aagacggtca gccgaaagat gccgcatggg
     8401 ctgaaaaact gaccggcatt gatgccgaaa ccattcgtgg gctggcgcgg cagatggcgg
     8461 cgaacagaac gcaaattatt gctggctggt gcgtgcagcg tatgcagcac ggtgaacagt
     8521 gggcgtggat gattgtggtt ctggcggcga tgctggggca aattggcctg ccaggtggtg
     8581 gttttggttt tggctggcac tacaacggcg caggcacgcc ggggcgtaaa ggcgttattc
     8641 tgagtggttt ctccggctct acgtcgattc cgcctgttca cgacaacagt gactacaaag
     8701 gctacagcag caccattccg attgcccgtt ttatcgatgc gatcctcgaa ccggggaaag
     8761 tgatcaactg gaacggtaaa tcggtaaaac tgccgccact gaaaatgtgt atttttgccg
     8821 gaactaaccc attccatcgc catcagcaga tcaaccgcat tatttaaggc tggcgcaagc
     8881 tggaaacggt tatcgccata gataaccagt ggacctcaac ctgccgcttt gccgatatcg
     8941 tactgcctgc gaccacgcag tttgagcgta acgatctcga ccagtacggc aaccactcca
     9001 accgtggcat tatcgccatg aaacaggtgg tgccgccgca gttcgaggcg cgcaacgact
     9061 ttgatatttt ccgcgagctg tgccgtcgct ttaatcgcga agaagccttt accgaagggc
     9121 tggacgaaat gggctggctg aaacgcatct ggcaggaagg tgtacaacaa ggcaaaggac
     9181 gcggcgttca tctgccagcg tttgatgact tctggaataa caaagagtac gtcgagtttg
     9241 accatccgca gatgtttgtt cgccaccagg cattccgcga agatccggat ctcgaaccgc
     9301 tgggcacgcc gagtggcctg attgagatct actcgaaaac catcgccgat atgaactacg
     9361 acgattgtca ggggcatccg atgtggtttg agaaaatcga acgctcccac ggtgggcccg
     9421 gctcgcagaa gtatccgttg catctgcaat ctgtgcatcc ggatttccga cttcactcgc
     9481 agttatgtga gtcggaaact ctgcgtcagc aatatacggt agcgggtaaa gagccagtat
     9541 tcattaaccc gcaggatgcc agcgcgcgcg gtattcgtaa cggtgatgtg gtacgcgtct
     9601 ttaacgctcg cggtcaggtg ttggcagggg cagtagtttc tgaccgctat gcacccggcg
     9661 tggcacgaat tcacgaaggg gcatggtacg atccagataa aggcggcgag cctggtgcgc
     9721 tgtgcaaata cggtaacccc aacgtgctga ccatcgacat cggtacttcg cagctggcgc
     9781 aggcgaccag tgcgcacact acgctggtgg aaattgagaa gtacaatgga gcagtggagc
     9841 aggtgacggc gtttaacggc cctgtggaga tagtggcgca gtgcgaatat gttcccgcgt
     9901 cgcaggtgaa atcatgacca cgctgacagc acaacagatt gcctgtgttt acgcctggct
     9961 ggcgcagttg ttctcccgtg aactggacga tgaacaactg acgcaaatcg ccagtgcgca
    10021 gatggcggaa tggttttcgt tgctgaaaag cgaaccgccg ctggctgcgg cggtgaatga
    10081 gctggaaaac tgtattgcca cgctgacagt acgtgacgat gcccgtctgg aactggccgc
    10141 tgacttttgc ggcctgtttt tgatgactga taagcaggcg gcgctgccgt atgcatcggc
    10201 ctacaaacag gacgagcaag agattaaacg cttgttagtt gaggccggga tggaaaccag
    10261 cggcaatttc aacgaaccgg cagaccatct ggcgatctat ctcgagttgt tgagtcatct
    10321 gcatttttcg ctgggagagg ggaccgttcc tgcgcgaaga atcgacagtt tgcggcaaaa
    10381 aacactgacg gcgctgtggc aatggttacc agagtttgct gcgcgttgcc atcagtatga
    10441 cagctttggt ttttacgcgg cactaagcca gttattgctg gtgttagtgg agtgcgacca
    10501 ccaaaacaaa taacgtcgtt tatgcgcctg aaaagacgcg tttagcgtcg catcaggcat
    10561 tatggcgcag ttgccggatg cgtcatcaca tccggcaaac acacatcacg gatgagctac
    10621 aaaccgggaa agccgctggc gtagcaggcg gttttcctgc ttcaggtgtg caatatcatc
    10681 cattaacgtc agcgccaccg cgatccccgg ccagtccaga gccagttcat gacgcaggcg
    10741 taccgcgcgt tgcaccacaa tggcggcatg gtcgtcaaat acccaggttg tttcctggat
    10801 ctcacgcggt tcaaccaccc ccaaaccgac aatttcattc aactcctctt cagagatgcc
    10861 ggtatgcagg caaaattcgg taatagtaaa agtcaccgta acattagcca tcatgctttc
    10921 ccccaatctt tacgtggatc aaaagacgac tgggcgtctg ccagttgctg ccacagcgcg
    10981 gcagtgtttt catccggttt cggcggcatc acgattttca gtaccgcata cagatcgccg
    11041 gtctgttttt tgctcaccag acctttgcct ttaacgcgca atcgttgccc ggcctggctg
    11101 cctggcggga tagtcagcaa aatgctttct ttcagtgttg gaacggtgac tttagcaccc
    11161 agcgccgctt cccacgggct aaccggcacc acaatttcca gatcctggcc gacaatatcg
    11221 aacagcggat gtggcgcaat atgaatcacc agccacaaat cgccatttgg accaccgttt
    11281 tcgcccggcg tcccctggcc tttcagacgg atgcgttgac cattgccgac gcccgccggg
    11341 atcttcacat tcagcgtttt cgggatttcc tgttcgatca tgccaaaggc gttataaacc
    11401 ggcaggttat agctgatggt acgcttatgc tcagtcagcg tttcttcgag gaataccgcc
    11461 acttcgattt caatatcgtg gccgcgtgcg gcggggcgtt gacggctctg gcgggcatgc
    11521 tgaccgaaaa ttgacgagaa gatatcgtca aaatcttcgg cattaaaact ctgaccgtcg
    11581 ccatgatgga actgacggtt aaattgcgga tcgttgcgat gttgccacat ctgatcatac
    11641 tcagcgcgac gttgttcatc acttaacact tcccaggctt cagcgacctc tttgaagcgg
    11701 gcttcggcat ccggttcttt gctgacatca ggatggtatt tacgggccag tcgacgatag
    11761 gcggtcttga ttgtcttgag atcgtccgtc ggtttcacgc ccatgatggc gtaataatcc
    11821 tttaattcca tagcgttatc tcgcgtaaat caacacaaat tgaaggaacc cctgtaaggt
    11881 aactcctata agtgtagggt aatcctcaaa atttcatatg ccaacacaga atatgttatt
    11941 gaaatcatcg tggagaggag gtcgccatca atatgggttg ctgaacatat tttaaacagg
    12001 tgaaaaaggg tgagcgattt ttgatagttg aaccaggcac tttaagttta actagggcgt
    12061 cattatttat taaat
//
LOCUS       DAJTMC010000125        11966 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.125, whole genome shotgun sequence.
ACCESSION   DAJTMC010000125 DAJTMC010000000
VERSION     DAJTMC010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11966
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.125"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(<1..412)
                     /locus_tag="OO130_003048"
     CDS             complement(<1..412)
                     /locus_tag="OO130_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCR5366365.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYVLTTQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVARRVTKAGYIALAPDGLSSV"
     gene            568..1608
                     /gene="gpr"
                     /locus_tag="OO130_003049"
     CDS             568..1608
                     /gene="gpr"
                     /locus_tag="OO130_003049"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HCR5366366.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGVSRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(1648..2142)
                     /locus_tag="OO130_003050"
     CDS             complement(1648..2142)
                     /locus_tag="OO130_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HCR5366367.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            2333..3217
                     /gene="yghA"
                     /locus_tag="OO130_003051"
     CDS             2333..3217
                     /gene="yghA"
                     /locus_tag="OO130_003051"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HCR5366368.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQQTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(3489..3914)
                     /gene="exbD"
                     /locus_tag="OO130_003052"
     CDS             complement(3489..3914)
                     /gene="exbD"
                     /locus_tag="OO130_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HCR5366369.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDLVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(3921..4655)
                     /gene="exbB"
                     /locus_tag="OO130_003053"
     CDS             complement(3921..4655)
                     /gene="exbB"
                     /locus_tag="OO130_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCR5366370.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLFNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            4907..6094
                     /gene="metC"
                     /locus_tag="OO130_003054"
     CDS             4907..6094
                     /gene="metC"
                     /locus_tag="OO130_003054"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HCR5366371.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFESMEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLSNEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            6234..6893
                     /locus_tag="OO130_003055"
     CDS             6234..6893
                     /locus_tag="OO130_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5366372.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGMLFVVIKKKYCN
                     A"
     gene            complement(6933..7832)
                     /gene="yqhC"
                     /locus_tag="OO130_003056"
     CDS             complement(6933..7832)
                     /gene="yqhC"
                     /locus_tag="OO130_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HCR5366373.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            8026..9189
                     /gene="yqhD"
                     /locus_tag="OO130_003057"
     CDS             8026..9189
                     /gene="yqhD"
                     /locus_tag="OO130_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HCR5366374.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAADNYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRDTKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            9294..10121
                     /gene="dkgA"
                     /locus_tag="OO130_003058"
     CDS             9294..10121
                     /gene="dkgA"
                     /locus_tag="OO130_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HCR5366375.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            10321..11247
                     /locus_tag="OO130_003059"
     CDS             10321..11247
                     /locus_tag="OO130_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HCR5366376.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     ADHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            11298..11555
                     /gene="yqhH"
                     /locus_tag="OO130_003060"
     CDS             11298..11555
                     /gene="yqhH"
                     /locus_tag="OO130_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HCR5366377.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(11590..11898)
                     /locus_tag="OO130_003061"
     CDS             complement(11590..11898)
                     /locus_tag="OO130_003061"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5366378.1"
                     /translation="MDYFCRNESVIDTATQKIIIQPGCHFARSSAHITSQIETEFIGY
                     WYIVITGQWLVQTFYRFINRFFAQHVSAEATEQQNKKTGVPASHNHVLMFGFFLANSL
                     "
BASE COUNT         3096 a         3042 c         2982 g         2846 t
ORIGIN      
        1 caacggaact taagccgtca ggtgccaggg cgatataccc cgccttcgtc actcgccgtg
       61 ccacatcttc gatatacgga ttcagtccac gattctcatg caccaccacc acggctggcg
      121 ttttgccgct catttttgcg ggtttcacca gataaccccg tacctcgccg tgaccatttg
      181 gcgaaggata cgtgatgtac tcagcaacaa tctccgggtc ggtaaactct acctgagtcg
      241 tcagcacata atttggcttg agcaaatcaa acaacgctaa tgccgtcatc ccgcccaccg
      301 catacttcgc cgcaaggttg aggaactcac gtttcgaaat tttcccgtga gcgtaatagt
      361 cgtagtaatc caacaactct tgtgggaaat ctttggcggt taaacgcggc atcgttgcat
      421 tcctcaactg gtgtttttta agcaaagcat aagcgcgtgt ttttgcccag tttttcgtca
      481 ctctgtgagc cagactacgg gatacgcgct ggcgaatcgc taaactagaa acattgtttc
      541 gaaattgaac ggtggaaagg agaggtcatg gtctggttag cgaatcccga acgttacggg
      601 cagatgcagt accgctattg cggaaaaagt ggtttacgcc tgcccgcgtt atcgctcggt
      661 ttatggcaca atttcggtca cgttaacgcg ctggaatcac agcgtgcaat cctgcgtaaa
      721 gcgtttgatt taggcattac gcactttgat ttagccaaca attacgggcc gcctccagga
      781 agcgcagaag agaactttgg tcgcctgctg cgggaggatt ttgccgctta tcgcgatgaa
      841 ctgattatct ctaccaaggc tggctacgat atgtggcccg gcccttacgg ctctggcggt
      901 tcacgtaaat acctgctcgc cagcctcgac caaagcctga agcgtatggg gcttgagtat
      961 gtcgatatct tttactctca tcgcgtcgat gaaaatacgc cgatggaaga aaccgcctct
     1021 gcgctggctc atgcggtaca aagcggtaag gcgctgtatg tcgggatctc ctcttactcg
     1081 ccagagcgga cgcaaaaaat ggtcgagttg ctgcacgagt ggaaaattcc gcttttaatt
     1141 catcaacctt cgtacaattt actgaaccgc tgggtggata aaagcggcct gctggatacc
     1201 ctgcaaaata acggcgtggg ctgtattgcc tttactcctc tggctcaggg attgctgacc
     1261 ggaaaatatc tcaacggcat tccgcaagat tcacggatgc atcgtgaagg gaataaagtt
     1321 cgtggtctga cgccgaaaat gcttaccgaa gccaacctca acagcctgcg cttattgaat
     1381 gaaatggcac agcagcgtgg acaatcaatg gcgcaaatgg cgttaagctg gttgctgaaa
     1441 gatgatcgcg tgacgtcggt attgattggt gtcagccgcg cggagcaact agaggagaac
     1501 gtgcaggcgc tgaataatct gacatttagc accgaggagc tggcgcagat tgatcagcat
     1561 atcgccgatg gcgagctgaa tctgtggcag gcgtcttccg ataaatgacc tgttaataac
     1621 gggcgcgcga accgcgcccg aatcagatca gtgattatga cgagtcagtc ggtcaagata
     1681 gcccatcaca aatgcagaga gcacaaacgt cagatggata atgacgtacc acatcagttt
     1741 gttatccggg acatttttcg catccataaa gacgcgcagt aagtgaatgg aagaaattgc
     1801 cacaatcgac gctgctactt tgtttttcag cgacgttgcg tccattttcc ccagccagtt
     1861 cagcttctct ttgttctcgg agatatccag ctgcgagacg aaattctcat aaccggaaaa
     1921 catcaccatc accagcaaac cgccaaccag cgtcatatcc accagcgaca gcaacacgag
     1981 gatcaaatct gattccgcca tcgagaagat attcggcagt acgtgaataa tctcctggaa
     2041 gaacttcagc gccagggcaa ctaacgccag cgaaaggcca aagtacacgg gggcaagcag
     2101 ccagcgagaa gcatacattg cattttcaag aaaacgttcc atacattcct gtcgtgtagt
     2161 taaaacgggc aacagtatat cgcaattgag taaacagcac gcaacagcaa atcaacatat
     2221 acgttaaacc tttgtcgcat aattctgagg tttagggatc ttcctggttc ccttatcaac
     2281 gcatcgagag cgtctaccct taaaagacgt tgaacctgaa gggagaaaaa caatgtctca
     2341 tttaaaagac ccgactacgc agtattacac tggtgaatat cccaaacaga aacaaccgac
     2401 gccaggcatc caggcgaaga tgacaccggt accggattgc ggcgagaaaa cctatgttgg
     2461 tagcggtcgc ctgaaagatc gtaaagcact ggtgacaggg ggcgattccg gaataggtcg
     2521 cgctgccgcc atcgcttacg cgcgtgaagg ggctgacgtg gcgatcagtt atcttcccgt
     2581 ggaagaagaa gacgctcagg atgtgaaaaa gatcattgaa gaatgcggac gcaaagccgt
     2641 tctgctgcca ggcgatttaa gcgatgagaa attcgcccgt tcgctggttc acgaagcgca
     2701 caaggcgtta ggcgggctgg atattatggc gctggtcgcc gggaaacagg ttgccattcc
     2761 ggatattgca gacctcacca gcgaacagtt tcaaaagacc tttgccatta acgttttcgc
     2821 gctgttctgg ctaacccagg aagcgatccc cctgctaccg aaaggtgcaa gtatcatcac
     2881 cacttcgtca atccaggcat accagccaag tccgcattta ctggactatg cggctacgaa
     2941 ggcggcgatt ctgaactaca gccgtggcct ggcaaaacag gtcgcggaga aaggtattcg
     3001 ggtgaatatt gtcgcgccag gccctatctg gacggcactg caaatttccg gcggacaaac
     3061 gcaggataag atcccgcagt ttggtcagca aacgccgatg aagcgtgcgg ggcaaccggc
     3121 ggaactggcc cctgtatatg tttatctggc aagtcaggag tcgagctacg tcaccgcaga
     3181 agtgcacggt gtgtgcggcg gcgagcattt aggttaaaaa aatgcccggt tgtgaaaagc
     3241 aaccgggcat cattgtgaat tcactcttac ctgatgtggc gaaaacgcct tatccgccta
     3301 caaacgcatg aagattcaac atgttgcaat agattttgta ggcctgataa gcgtagcgca
     3361 tcaggcattg agcacctaat gccggatgcg gcgtgaacgc cttatccggc ctacaaacgc
     3421 acgaagattc aacatgttgc aatagatttt gtaggcctga tgagcgtagc gcatcaggca
     3481 attctacttt acttcgcttt ggcggtttct tcgccgacca gacctatctt caggtaaccc
     3541 gcctgatgca gcgtatccat taccttcatc aacgtctcgt aatcgacggt tttatccgct
     3601 cggaagaaga tggtggtgtc tttcttgcct tcggttaacg cattcaacgc cgtaatcatt
     3661 gtttcatcgg tgaccagatc gttaccgata aacatcgagt tgtctgcctt caccgacaga
     3721 taaaccggtt tttccgggcg cggctgcggc gtgctggtag aagcaggcaa gttcaccttc
     3781 acatctaccg tcgctaacgg tgccgccacc ataaagataa tcagcagaac caacatcacg
     3841 tcgataaacg gcgtcacgtt gatatcatgc atttcgccgt tatcgtcgag gttttcgtta
     3901 agatgcattg ccatcggata ttatcctgcg cgtaattttt gtgcgacacg aaccggatgc
     3961 gcagcggcgc tggcttccag atccaggtca cggctttgca gcaacaatac ctgcgctgca
     4021 acatcaccca gcatcgcttt aaagccgcca atctggcgtg caaatacgtt atagataacg
     4081 accgcaggaa tcgctgccac gaggccgatt gccgttgcta acagagcttc tgcgataccc
     4141 ggcgcaacga ctgccaggtt agtggtctgc gtttgcgcga taccaataaa gctgttcatg
     4201 atgccccaga ccgtaccaaa cagaccaacg aacggagaaa tcgcgccgat ggttgccaga
     4261 tagccgttac cgcggcccat ttgacgaccc actgcggcga cccgacgctc cagacggaag
     4321 ctggtacgtt ctttaatacc ttcgttatcg tcgctgcctt ctgacagttc cagctcattc
     4381 tgcgcttcat tgaacaaatg caggcttaag cttttgctac caaaatcagc ggcgatatcg
     4441 ttggcctggt ttaaggaacg cgcttcagcc agcagttgct gctcgcgctt taggcgacgc
     4501 ttctgattga agaactctac gctcttactg aagaagattg cccaggtgac tacggaggcc
     4561 aaaataagcc caatcatcac gcacttaacg acaatatcgg cgtgctgata cataccccag
     4621 acggaaaggt ctgtctgcat taaattatta cccactgtgt atctccagga cgcaagtcac
     4681 aaaatctgcg cataataata tcaaaacgac gtcgaattga tagtcattct cattactatt
     4741 tgcatactgc cgtacctttg ctttcttttc cttgcgttta cgcagtaaaa aagtcaccag
     4801 cacgccattt gcgaaaattt tctgctttat gccaattctt caggatgcgc ccgcgaatat
     4861 tcatgctagt ttagacatcc agacgtataa aaacaggaat cccgacatgg cggacaaaaa
     4921 gcttgatact caactggtga atgcaggacg cagcaaaaaa tacactcttg gcgcggtaaa
     4981 tagcgtgatt cagcgcgctt cttcgctggt atttgagagt atggaagcca aaaagcacgc
     5041 gacgcgcaat cgcgccaatg gcgagttgtt ctatggacgg cgcggaacgt taacccattt
     5101 ttccttacaa caagcgatgt gtgaactgga aggtggcgca ggctgcgcgc tatttccctg
     5161 cggggcggca gcggttgcta attcgattct tgcttttgtc gaacagggcg atcatgtgtt
     5221 gatgaccaac actgcctatg aaccgagtca ggatttctgt agcaaaatcc tcagcaaact
     5281 gggcgtaacg acatcgtggt ttgatccgct gattggtgcc gatatcgtta agcatctgca
     5341 gccaaacact aaaatcgtgt ttctggaatc gccaggctcc atcaccatgg aagtccacga
     5401 cgttccggcg attgttgccg ccgtacgcag tgtggtgccg gatgccatca ttatgatcga
     5461 caacacctgg gcagccggtg tgctgtttaa ggcactggat tttggcatcg atgtttctat
     5521 tcaagccgcc accaaatatc tggttgggca ttcagatgcg atgattggca ctgccgtgtg
     5581 caatgcccgt tgctgggagc agctacggga aaacgcctat ctgatgggcc agatggtcga
     5641 tgccgatacc gcctatataa ccagccgtgg cctgcgcaca ttaggtgtgc gtttgcgtca
     5701 acatcatgaa agcagtctga aagtggctga atggctggca gaacatccgc aagtagcgcg
     5761 agttaaccac cctgctctgc ctggcagtaa aggacacgaa ttctggaaac gagactttac
     5821 aggtagcagc gggctgtttt cctttgtgct taagaaaaaa ctcagtaatg aagagctggc
     5881 gaactatctg gataacttca gtttattcag catggcctac tcgtggggcg ggtatgaatc
     5941 gttgatcctg gcgaatcaac cagaacatat cgccgccatt cgcccacaag gcgagatcga
     6001 ttttagcggg accttgattc gcctgcatat tggtctggaa gatgtcgacg atctgattgc
     6061 cgatctggac gccggttttg cgcgaattgt ataacattgc cacttttgga caattttgca
     6121 gacatttaat tgtgaaaagt cttaaattgt tgcgtccggg atcaaggcgt cccggacgaa
     6181 tcaggagtac aataggcaga taaaggctta aacgctgttc cacaggaaag tccatggctg
     6241 ttattcaaga tatcatcgct gcgctctggc aacacgactt tgccgcgctg gcggatcctc
     6301 atattgttag cgttgtttac tttgtcatgt ttgccacgct gtttttagaa aacggcctgc
     6361 tgcccgcctc atttttgcca ggcgacagct tgttgatact ggcaggcgca ttgattgccc
     6421 agggggttat ggattttctg cctacgattg cgattctgac cgccgcagca agtctgggct
     6481 gctggctaag ttatattcag gggcgctggt tagggaatac caaaacggtg aaaggctggc
     6541 tggcacagct tcctgctaaa tatcaccagc gcgccacctg catgtttgac cgccacggtc
     6601 tgctggcgct gctggctgga cgttttcttg catttgtccg tacgctgctg ccaaccatgg
     6661 cgggaatttc cggtctgcca aaccgccgct tccagttttt caactggtta agtggattgc
     6721 tgtgggtcag cgtggtaacc agttttggct atgccttaag tatgattccg ttcgttaaac
     6781 gccatgaaga tcaggtaatg acgttcctga tgatcctgcc aattgccttg ttaaccgctg
     6841 gcttgttagg catgctgttt gtggtgatta aaaaaaaata ctgtaacgcc tgacgatttt
     6901 ccccgttccc ggctgctgta ccgggaacgt atttaattcc cctgcatcgc ccgcattctt
     6961 gccgcatctt cccccggcgt cacaccgaag taacgtttaa actcacggct aaattgcgat
     7021 gcgctttcat agccgacgcg catcgctgct gcgctggcct tcatgccgtc atggatgatc
     7081 atcatccgcg ccttatgcag acggtaattc ttcaaatact gcaacggcga ggtgctggtg
     7141 acagacttaa aattatggtg gaacgccgat acgctcatgt tggcttctgc cgccagttgc
     7201 tcgacgctca ggttttcggt gtatttattc tcaatccgtt tcagcacgcg gctaatcaga
     7261 ctgaagtgag tctggcgact gaccagcgcc agtaacgcgc cgccgcaagg tccggtcagc
     7321 acgtagtaca gaatttcgcg gatgatctgt ttgccgagaa tacgcgcatc cagtggtcgc
     7381 tccatcacgt cgagtaaccg ctccgccgcg cataaaatct cttctgataa cgtggcggag
     7441 ttaatcccgc tggctgccat cgacggctgg aaatgctcat cttcgccaat gtccatcaac
     7501 agttcctgta actgcaaaat atcgacattg agacgcaacc ctgccagcgg cacctctgac
     7561 gtggcatagg tttcgcactc aaacggcaac ggcaccgtca gcagcaggta ttcattggca
     7621 tcataacgaa acacgcgttc attgatataa ccgattttat gcccggaaaa gagaattatg
     7681 atgccaggct cgtacatcac cggtgtacgt gcgaaaggcg tctcaccata caacaaacgc
     7741 acatcgggca acagttctga caaactattt tctttatttt tcagtttatt aactttatcc
     7801 gccagcaagc ggcaaatctc ttcacgtttc atatcgcgta atttcttagg aataatgcgg
     7861 caatttgatt gtgcgcaatt ttgtagcatt tctccagcac tctggagaaa taggcaagac
     7921 attggcagaa atgagcattg agagccaggg cgctggcgat cacaatgaaa aacatcaggc
     7981 agatcgttct ctgccctcat attggcccag caaagggagc aagtaatgaa caactttaat
     8041 ctgcacaccc caacccgcat tctgtttggt aaaggcgcaa tcgctggttt acgcgaacaa
     8101 attcctcacg atgctcgcgt attgattacc tacggtggcg gcagcgtgaa aaaaaccggc
     8161 gttctcgatc aagttctgga tgccctgaaa ggcatggacg tgctggaatt tggcggtatt
     8221 gagccaaacc cggcttatga aacgctgatg aacgccgtga aactggttcg cgaacagaaa
     8281 gtgactttcc tgctggcggt tggcggcggt tctgtactgg acggcaccaa atttatcgcc
     8341 gcagcggata actatccgga aaatatcgat ccgtggcaca ttctgcaaac gggcggtaaa
     8401 gagattaaaa gcgccatccc gatgggctgt gtgctgacgc tgccagcaac cggttcagaa
     8461 tccaacgcag gcgcggtgat ctcccgtaaa accactggcg acaagcaggc gttccattct
     8521 gcccatgttc agccggtatt tgccgtgctc gatccggttt atacctacac cctgccgccg
     8581 cgtcaggtgg ctaacggcgt agtggacgcc tttgtacaca ccgtggaaca gtatgttacc
     8641 aaaccggttg atgccaaaat tcaggaccgt ttcgcagaag gcattttgct gacgctgatc
     8701 gaagatggtc cgaaagccct gaaagagcca gaaaactacg atgtgcgcgc caacgtcatg
     8761 tgggcggcga cgcaggcgct gaacggtttg attggcgctg gcgtaccgca ggactgggca
     8821 acgcatatgc tgggccacga actgactgcg atgcacggtc tggatcacgc gcaaacactg
     8881 gctatcgtcc tgcctgcact gtggaatgaa aaacgcgata ccaagcgcgc taagctgctg
     8941 caatatgctg aacgcgtctg gaacatcact gaaggttccg acgatgagcg tattgacgcc
     9001 gcgattgccg caacccgcaa tttctttgag caattaggcg tgccgaccca cctctccgac
     9061 tacggtctgg acggcagctc catcccggct ttgctgaaaa aactggaaga gcacggcatg
     9121 acccaactgg gcgaaaatca tgacattacg ttggatgtca gccgccgtat atacgaagcc
     9181 gcccgctaag ctttttacgc ctcaaacttt cgttttcggg catttcgtcc agacttaagt
     9241 tcacaacacc tcaccggagc ctgctccggt gagttcatat aaaggaggaa cgtatggcta
     9301 atccaaccgt tattaagcta caggatggca atgtcatgcc ccagctggga ctgggcgtct
     9361 ggcaagcaag taatgaggaa gtaatcaccg ccattcaaaa agcgttagaa gtgggttatc
     9421 gctcgattga taccgccgcg gcctacaaga acgaagaagg tgtcggcaaa gccctgaaaa
     9481 atgcctcagt caacagagaa gaactgttca tcaccactaa gctgtggaac gacgaccaca
     9541 agcgcccccg cgaagccctg ctcgacagcc tgaaaaaact ccagcttgat tatatcgacc
     9601 tctacttaat gcactggccc gttcccgcta tcgaccatta tgtcgaagca tggaaaggca
     9661 tgatcgaatt gcaaaaagag ggattaatca aaagcatcgg cgtgtgcaac ttccagatcc
     9721 atcacctgca acgcctgatt gatgaaactg gcgtgacgcc tgtgataaac cagatcgaac
     9781 ttcatccgct gatgcaacaa cgccagctac acgcctggaa cgcgacacac aaaatccaga
     9841 ccgaatcctg gagcccatta gcgcaaggag ggaaaggcgt tttcgatcag aaagtcattc
     9901 gcgatctggc agataaatac ggcaaaaccc cggcgcagat tgttatccgc tggcatctgg
     9961 atagcggcct ggtggtgatc ccgaaatcgg tcacaccttc acgtattgcc gaaaactttg
    10021 atgtctggga tttccgtctc gacaaagacg aactcggcga aattgcaaaa ctcgatcagg
    10081 gcaagcgtct cggtcccgat cctgaccagt tcggcggcta acatgcaaat tctcccggtg
    10141 gcggtaatgt tccgctaccg gacttttcag aaatcattta ttcccctcgc gtcccgcccg
    10201 ttgttactct tccttgttca ggaatgccaa atataaggac atcatcatgc agagccggaa
    10261 gctcttaaaa gaacaactca tctatatccg ggataaacgc aacggagagg tgaaaaacag
    10321 atgaaaataa tacttctgtt tttagcagcc ctggcaagtt ttaccgtaca cgcgcagcct
    10381 ccttcacaga ccgtagaaca aacagtccgg cagatttatc agaactataa atcagatgcc
    10441 agtacgcctt atttcggtga aaccggagag cgggcgataa cttcagcgcg tattcaacag
    10501 gcgcttaccc tgaacgacaa tcttacgctg ccgggcaata ttggctggct ggattatgat
    10561 ccggtttgtg attgtcagga ttttggcgat ctggtgctag aaagcgttgc gataacccaa
    10621 actgacgccg atcatgccga tgccgttgtg cgctttcgta tctttaaaga tgataaagaa
    10681 aagaccacgc agacactgaa aatggtggcg gaaaatggtc gttgggtcat tgacgatatt
    10741 gtcagcaatc atggcagtgt cttacaagca gttaatagcg agaatgaaaa aacgctggcc
    10801 gctttagctt cgttgcaaaa agaacagccg gaagcctttg ttgccgaact ctttgaacat
    10861 attgctgatt atagctggcc gtggacgtgg gtggtttccg actcttaccg ccaggcggtt
    10921 aatgccttct ataaaaccac cttcaagacg gccaataatc ccgatgaaga tatgcaaata
    10981 gaacggcaat ttatttacga caatccgatc tgttttggcg aagagtcgct attttcacgc
    11041 gttgatgaaa ttcgagtcct ggagaaaacc gccgattccg cccgcattca tgttcgtttt
    11101 acgctgacca atggcaacaa cgaagagcaa gaactggttt tacagcggcg cgaaggcaag
    11161 tgggaaatcg ctgattttat ccgcccgaac agcggcagcc tacttaagca gattgaggca
    11221 aaaactgccg ccagattaaa gcaatgagct gaattaaata acaattagcc ggaacaataa
    11281 ataaaaggga acactatatg aaaacgattt tcaccgtggg agctgttgtt ctggcaacct
    11341 gcttgctcag tggctgcgtc aatgagcaaa aggtcaatca gctggcgagc aatgtgcaaa
    11401 cattaaatgc caaaatcgcc cggcttgagc aggatatgaa agcactacgc ccacaaatct
    11461 atgctgccaa atccgaagct aacagagcca atacgcgtct tgatgcacag gactattttg
    11521 attgcctgcg ctgcttgcgt atgtacgcag aatgataaaa aaatccccgg cagcatgtca
    11581 gttgccgggt tataggctgt ttgctaaaaa aaagccaaac ataaggacat gattatgcga
    11641 cgctggcaca cctgtttttt tattctgctg ttctgtggct tcagcggaaa cgtgttgtgc
    11701 aaagaatcga ttaatgaagc gatagaacgt ttgtaccaac cattgaccag taatgacgat
    11761 ataccaataa ccgatgaatt cagtctcgat ttgcgaagta atatgagcgc tactcctggc
    11821 aaaatgacac cctggctgaa taataatctt ttgtgtcgct gtgtcgatga cgctttcgtt
    11881 tctgcagaaa taatccaaag cacacaccta aatggcggcc gaacgcatgt actgttgcag
    11941 ctggttatca aacaaaatcg cacaga
//
LOCUS       DAJTMC010000126        11926 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.126, whole genome shotgun sequence.
ACCESSION   DAJTMC010000126 DAJTMC010000000
VERSION     DAJTMC010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11926
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.126"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            216..1325
                     /gene="livK"
                     /locus_tag="OO130_003062"
     CDS             216..1325
                     /gene="livK"
                     /locus_tag="OO130_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HCR5366379.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            1373..2299
                     /gene="livH"
                     /locus_tag="OO130_003063"
     CDS             1373..2299
                     /gene="livH"
                     /locus_tag="OO130_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HCR5366380.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            2296..3573
                     /gene="livM"
                     /locus_tag="OO130_003064"
     CDS             2296..3573
                     /gene="livM"
                     /locus_tag="OO130_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HCR5366381.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWVFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTFFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            3570..4337
                     /gene="livG"
                     /locus_tag="OO130_003065"
     CDS             3570..4337
                     /gene="livG"
                     /locus_tag="OO130_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HCR5366382.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            4339..5052
                     /gene="livF"
                     /locus_tag="OO130_003066"
     CDS             4339..5052
                     /gene="livF"
                     /locus_tag="OO130_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HCR5366383.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRAASGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            5449..6120
                     /locus_tag="OO130_003067"
     CDS             5449..6120
                     /locus_tag="OO130_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000803216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCR5366384.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYAAKLKASGMKCGYASGWQGWIQLENRHLPGDRRRHVRRAGSGH
                     HDADL"
     gene            6050..6253
                     /locus_tag="OO130_003068"
                     /pseudo
     CDS             6050..6253
                     /locus_tag="OO130_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="with UgpEC is involved in the uptake of
                     glycerol-3-phosphate; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter permease"
     gene            6250..7095
                     /gene="ugpE"
                     /locus_tag="OO130_003069"
     CDS             6250..7095
                     /gene="ugpE"
                     /locus_tag="OO130_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HCR5366385.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATTTF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            7097..8167
                     /gene="ugpC"
                     /locus_tag="OO130_003070"
     CDS             7097..8167
                     /gene="ugpC"
                     /locus_tag="OO130_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HCR5366386.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTEGDIWINDQRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVRDPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLTGRVNNEGTHFELDGGIELPLNGGYRQYAGRKM
                     TLGIRPEHIVLSSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGS
                     TLWLHLAENQLHLFDGETGQRV"
     gene            8164..8907
                     /gene="ugpQ"
                     /locus_tag="OO130_003071"
     CDS             8164..8907
                     /gene="ugpQ"
                     /locus_tag="OO130_003071"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCR5366387.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVVGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQLAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(8894..9334)
                     /locus_tag="OO130_003072"
     CDS             complement(8894..9334)
                     /locus_tag="OO130_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HCR5366388.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            9454..11187
                     /gene="ggt"
                     /locus_tag="OO130_003073"
     CDS             9454..11187
                     /gene="ggt"
                     /locus_tag="OO130_003073"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HCR5366389.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAVAAPPVSYGVEEDIFHPVRAK
                     QGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIRSKN
                     GNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDKYGT
                     MPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKKGDK
                     LVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERTPIS
                     GDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAYADR
                     SEYLGDPDFVKVPWQALTNKAYAKSIADQIDINKAQPSSEIRPGKLAPYESNQTTHYS
                     VVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGDANA
                     VGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEATNA
                     PRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYGASD
                     PRSVDDLTAGY"
     gene            complement(11225..11509)
                     /locus_tag="OO130_003074"
                     /pseudo
     CDS             complement(11225..11509)
                     /locus_tag="OO130_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417903.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YrhB family protein"
     gene            complement(11709..11864)
                     /locus_tag="OO130_003075"
     CDS             complement(11709..11864)
                     /locus_tag="OO130_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5366390.1"
                     /translation="MEKLTTELHSLSEMDRRHVVSILAEIANGYDDFNDMLIYLEFYP
                     NHKILIS"
BASE COUNT         2707 a         2955 c         3388 g         2876 t
ORIGIN      
        1 aaaggcactt ttttctgttt atctatcaat aaattcagaa tattatctgt tcttaatcga
       61 ctgaaagata gggattttaa tcactattat cacaaaatac tgcgctaaac ccttaatcag
      121 acaggcaaaa acagtgcagt ataaaaaaag aacagtctga tttgttaaca cataaaaaca
      181 aagcaacaca acatcacgaa tggggatttt tgactatgaa acggaatgcg aaaactatca
      241 tcgcagggat gattgcactg gcaatttcac acaccgctat ggctgacgat attaaagtcg
      301 ccgttgtcgg cgcgatgtcc ggcccgattg cccagtgggg cgatatggaa tttaacggcg
      361 cgcgtcaggc gattaaagac attaatgcca aagggggaat taagggcgac aagctggttg
      421 gcgtggaata tgacgacgcc tgcgacccga aacaagccgt tgcggtcgcc aacaaaatcg
      481 ttaatgacgg cattaaatac gttattggtc atctgtgttc ttcttctacc caacctgcat
      541 cagatatcta cgaagacgaa ggtattctga tgatctcacc gggagcgacc aacccggagc
      601 tgacccaacg cggttatcaa cacattatgc gtactgccgg gctggactct tcccaggggc
      661 caacggcggc aaaatacatt cttgagacgg tgaagcccca gcgcatcgcc atcattcacg
      721 acaaacaaca gtatggcgaa gggctggcgc gttcggtgca ggacgggctg aaagcggcta
      781 acgccaacgt cgtcttcttc gacggtatta ccgccgggga gaaagatttc tccgcgctga
      841 tcgcccgcct gaaaaaagaa aacatcgact tcgtttacta cggcggttac tacccggaaa
      901 tggggcagat gctgcgccag gcccgttccg ttggcctgaa aactcagttt atggggccgg
      961 aaggtgtggg taacgcatca ttgtcgaata ttgccggtga tgctgccgaa ggcatgttgg
     1021 tcactatgcc aaaacgctat gaccaggatc cggcaaacca gggcatcgtt gatgcgctga
     1081 aagcagacaa gaaagatccg tccgggcctt atgtctggat cacctacgcg gcggtgcaat
     1141 ctctggcgac tgcccttgag cgtactggca gcgatgagcc gctggcgctg gtgaaagatt
     1201 taaaagctaa cggtgcaaac accgtgattg ggccgctgaa ctgggatgaa aaaggcgatc
     1261 ttaagggatt tgattttggt gtcttccagt ggcacgccga cggttcatcc acggcagcca
     1321 agtgatcatc ccaccgcccg taaaatgcgg gcgggtttag aaaggttacc ttatgtctga
     1381 gcagtttttg tatttcttgc agcagatgtt taacggcgtc acgctgggca gtacctacgc
     1441 gctgatagcc atcggctaca ccatggttta cggcattatc ggcatgatca acttcgccca
     1501 cggcgaggtt tatatgattg gcagctacgt ctcatttatg atcatcgccg cgctgatgat
     1561 gatgggcatt gataccggct ggctgctggt ggcggcggga ttcgtcggcg caatcgtcat
     1621 tgccagcgcc tacggctgga gtatcgaacg ggtagcctat cgcccggtgc gtaactctaa
     1681 gcgcctgatt gcgctcatct cggcaatcgg catgtccatc ttcctgcaaa actacgttag
     1741 cctgactgaa ggttcgcgtg acgtggcgct gcccagtctg tttaacggtc agtgggtggt
     1801 ggggcatagc gaaaacttct ctgcctctat taccaccatg caggcagtga tctggattgt
     1861 tactttcctc gccatgctgg cgctgacgat tttcattcgt tattcccgta tggggcgcgc
     1921 ctgccgtgcc tgcgcggaag atctgaaaat ggcgagcctg cttggcatca acaccgaccg
     1981 ggtgattgct ttgacctttg ttattggcgc ggcgatggcg gcagtggctg gcgtactgct
     2041 cggtcagttc tacggcgtca ttaaccccta tatcggcttt atggccggga tgaaagcctt
     2101 taccgctgcg gtgctcggtg ggattggcag cattccaggg gcgatgattg gtgggctgat
     2161 cctggggatt gcggaggcgc tctcttctgc ctatctgagt acggaatata aggatgtggt
     2221 ttcatttgcc ctgctgattc tggtgctgct ggtgatgccg accggtattc tgggtcgccc
     2281 ggaggtagag aaagtatgaa accgatgcat attgcaatgg cgctgctctc tgccgcgatg
     2341 ttttttgtcc tggcgggcgt ctttatgggc gtgcaactgg agctggatgg caccaaactg
     2401 gtggtcgaca cggcttcaga catccgttgg cagtgggtgt ttatcggcac ggcggtggtc
     2461 tttttcttcc agcttttgcg accggctttc cagaaagggc taaaaagcgt ttccggaccg
     2521 aagtttattc tgcccgcgat tgatggctcc acggtgaagc agaaactgtt cctcgtggcg
     2581 ctgttggtgc ttgcggtggc gtggccgttt atggtgtcac gtgggacggt ggatatcgcc
     2641 actctgacca tgatctacat tatcctcggt ctcgggctga atgtggttgt tggtctttct
     2701 ggtctgctgg tgctggggta cggcggtttt tacgccatcg gcgcttacac ctttgcgctg
     2761 ctcaatcact attacggctt gggcttctgg acttgtctgc cgattgccgg gttaatggca
     2821 gcggcggcgg gcttcctgct cggctttccg gtgctgcgtc tgcgcggtga ttatctggcg
     2881 atcgttaccc tcggcttcgg cgaaattgtg cgcatattgc tgctcaataa caccgaaatt
     2941 accggcggcc cgaacggaat cagtcagatc ccgaaaccga ctttcttcgg ccttgagttc
     3001 agccgtaccg cccgtgaagg cggttgggac acgttcagta atttctttgg cctgaaatac
     3061 gatccctccg atcgcgtcat cttcctgtat ctggtggcgt tgctgctggt ggtgctaagc
     3121 ctgtttgtca ttaaccgcct gctgcggatg ccgctggggc gtgcgtggga agcgttgcgt
     3181 gaagatgaga tcgcctgccg ttcgctgggc ttaagcccgc gtcgtatcaa gctgaccgct
     3241 tttaccatca gtgccgcgtt tgccggtttt gccggaacgc tgtttgctgc gcgccagggc
     3301 tttgtcagcc cggaatcctt cacctttgct gaatcggcgt ttgtactggc gatagtggtg
     3361 ctcggcggta tgggatcgca gtttgcggtg attctggcgg caattttgct ggtggtgtcg
     3421 cgcgagttga tgcgtgattt caacgaatac agcatgttaa tgctcggtgg tttgatggtg
     3481 ctgatgatga tctggcgtcc gcagggcttg ctgcccatga cgcgcccgca actgaagctg
     3541 aaaaacggcg cagcgaaagg agagcaggca tgagtcagcc attattatct gtgaacggcc
     3601 tgatgatgcg cttcggcggc ctgctggcgg tgaacaacgt caatcttgaa ctgtacccgc
     3661 aggagatcgt ctcgttaatc ggtcctaacg gtgccggaaa aaccacggtc tttaactgcc
     3721 tgaccggatt ctacaaaccc actggaggca ccattttact gcgcgatcag catctggaag
     3781 gtttaccggg gcagcaaatt gcccgcatgg gcgtggtgcg taccttccag catgtgcgtc
     3841 tgttccgtga aatgacggtg attgaaaacc tgctggtggc gcagcatcag caactgaaaa
     3901 ccgggctgtt ctctggcctg ttgaaaacgc catccttccg tcgcgcccag agcgaagcgc
     3961 tcgatcgcgc cgcgacctgg cttgagcgca ttggtttgct ggaacacgcc aaccgtcagg
     4021 cgagtaacct ggcctatggt gaccagcgcc gtcttgagat tgcccgctgc atggtgacgc
     4081 agccggagat tttaatgctc gacgaacctg cggcaggtct taacccgaaa gagacgaaag
     4141 agctggatga gctgattgcc gaactgcgca atcatcacaa caccactatc ttgttgattg
     4201 aacacgatat gaagctggtg atgggaattt cggaccgaat ttacgtggtc aatcagggga
     4261 cgccactggc aaacggtacg ccggagcaaa tccgtaataa cccggacgtg atccgtgcct
     4321 atttaggtga ggcataagat ggaaaaagtc atgttgtcct ttgacaaagt cagcgcccac
     4381 tacggcaaaa tccaggcact gcatgaggtg agcctgcata tcaatcaggg cgagattgtc
     4441 acgctgattg gcgcgaacgg ggcggggaaa accaccttgc tcggcacgtt atgcggcgat
     4501 ccgcgcgccg ccagcgggcg aattgtgttt gatgataaag acattaccga ctggcagacg
     4561 gcgaaaatca tgcgcgaagc ggtagcgatt gtcccggaag ggcgtcgcgt cttctcgcgg
     4621 atgacggtgg aagagaacct ggcgatgggc ggcttttttg ccgaacgcga ccagttccag
     4681 gagcgcataa agtgggtgta tgaactgttt ccacgtctgc atgagcgccg tgttcagcgg
     4741 gcaggcacca tgtccggcgg tgagcaacaa atgctggcga ttggtcgcgc gctgatgagc
     4801 aacccgcgtt tgctgctact tgatgagcca tcgctcggtc ttgcgccgat tatcatccag
     4861 caaattttcg acaccatcga gcagctgcgc gagcagggga tgactatctt cctcgtcgag
     4921 cagaacgcca accaggcgct aaagctggcg gatcgcggct acgtgctgga aaacggccat
     4981 gtggtgctct ccgataccgg cgatgcgctg ctggcgaacg aagcagtgag aagtgcgtat
     5041 ttaggcgggt aataacacgt tgattgataa ggagtcaaaa gactcctttg agacaggtga
     5101 caaatgtaaa attgcctgat gcgctgcgct tatcaggcct actgggtgag ttgcaatata
     5161 ttgaatttgc acgatcttgt aggccggata aggcgtttac gccgcatccg gcatgaaacg
     5221 atgagtaatc tgtagagttt gattcagacc ttctattttt ccgcttatcc gtgccccatc
     5281 tcccattttc cctcacccac gccgtcaccg ccttgtcatc tttctgacac cttactatct
     5341 tacaaatgta acaaaaaagt tatttttctg taattcgagc atgtcatgtt accccgcgag
     5401 cataaaacgc gtgaattcgc gcattcggta caacaagaga gataaacgat gaaaccgtta
     5461 cattatacag cttcagcact ggcgctcgga ctggcgttaa tggggaatgc acaggcagtg
     5521 acgaccattc cgttctggca ttctatggaa ggggaactgg gtaaagaggt ggattctctg
     5581 gcccaacgtt ttaacgccga aaatccggat tacaaaattg taccgaccta taaaggcaac
     5641 tacgaacaga atttaagcgc ggggattgcc gcatttcgta ccggcaacgc tccggctatt
     5701 ttgcaggttt atgaagttgg caccgccacc atgatggcat cgaaagccat taaaccggta
     5761 tatgacgtgt ttaaagaggc ggggattcaa ttcgatgagt cgcagtttgt gccgacggtt
     5821 tccggttact actccgacag caaaacgggc cacttactct cccagccatt caacagctcg
     5881 actcccgttc tctattacaa caaagacgcc ttcaagaaag cggggttaga cccggaacag
     5941 ccgccgaaaa cctggcagga tctggcggac tatgccgcga aactgaaagc ctccggcatg
     6001 aagtgcggct acgccagcgg ctggcagggc tggatccaac tggaaaatcg acaccttccc
     6061 ggtgatcgac gccgccacgt ccggcgggcc ggttcaggcc accacgacgc tgatttataa
     6121 gatctaccgc gaaggtttta ccggactgga tctggcttcg tctgccgcac agtcggtggt
     6181 gttgatgttc ctcgtcatcg tgctgacggt ggtgcagttc cgctatgttg aaagcaaggt
     6241 gcgttaccaa tgattgagaa ccgtccgtgg ctgacgatat tcagccatac catgctgatc
     6301 ctcgggatcg cggtgattct cttcccgctg tacgtggcgt ttgtcgcggc gacgctggat
     6361 aaacaggccg tctatgccgc gccgatgacg ctcatccccg gcacacatct gctggaaaat
     6421 atccacaaca tctgggttaa cggggtaggt acgaatagcg cgccgttctg gcggatgttg
     6481 cttaacagct ttgtgatggc gttcagcatt acgctcggca aaattaccgt ctcgatgctc
     6541 tcggcatttg ccattgtctg gtttcgtttt ccgctacgta acctcttctt ctggatgatt
     6601 tttatcaccc tgatgctgcc ggttgaagta cgtatcttcc cgacggtgga agtcatcgcc
     6661 aacctgaaga tgctcgacag ctacgccggt ttaacgctgc cgctgatggc ctcggcgacc
     6721 accacgtttc tgtttcgcca gttctttatg acgctgccgg atgaactggt ggaagcggcg
     6781 cggatcgacg gcgcatcgcc aatgcgcttc ttttgcgaca tcgtttttcc gctctccaaa
     6841 actaatctgg cggcgctgtt tgtgatcacc tttatttacg gctggaacca gtatttgtgg
     6901 ccgttgttga ttattaccga cgtggatctc ggcaccaccg tggcagggat caaagggatg
     6961 atcgccactg gcgaaggcac cacggaatgg aactcagtga tggcggcgat gttgttaacc
     7021 ctgatcccac cagtagtgat tgttttagtg atgcagcgtg ccttcgtgcg cggcctggtc
     7081 gatagtgaga aataagatgg caggactgaa attacaggca gtaaccaaaa gctgggatgg
     7141 caaaacccag gtcattaaac cgctgaccct tgatgtggct gatggcgaat ttatcgtgat
     7201 ggtcgggccg tctggctgcg ggaaatcgac gctgctgcgt atggttgccg ggctggagcg
     7261 ggtgaccgaa ggcgatatct ggatcaacga ccagcgcgtg actgaaatgg agccgaaaga
     7321 tcgcgggatt gcgatggtgt tccagaacta cgcgctttat ccgcatatga gcgtcgaaga
     7381 aaacatggcg tgggggctta aaattcgcgg catgggcaag cagcaaattg ccgagcgcgt
     7441 taaagaagcg gcgcgcattc tggagctgga cggtctgctc aaacgtcgcc cgcgtgagct
     7501 atccggcggt cagcgtcagc gtgtagcgat gggccgcgcg attgtgcgcg atccggcggt
     7561 gttcctgttt gatgagccgc tctctaacct cgatgccaag ctgcgcgtgc agatgcgtct
     7621 tgaactgcaa cagttgcacc gtcgcctgaa aacgacttca ctctacgtta ctcacgatca
     7681 ggttgaagcg atgacgctcg cccagcgagt aatggtgatg aacggcggcg ttgccgaaca
     7741 gattggcaca ccagttgaag tctacgaaaa gcccgccagc ctgtttgtag cgagttttat
     7801 cggcagtccg gcgatgaacc tgctgacagg ccgcgtgaat aacgaaggca cgcatttcga
     7861 actggacggc ggtattgagc tgccgctaaa cggtggctac cgtcagtatg ccgggcgtaa
     7921 aatgactctc ggcattcgcc cggaacatat tgtgctaagc tcgcaggcag aaggcggcgt
     7981 accgctggtg atggacacgc tggagatcct cggcgcagat aacctggcgc acggacgctg
     8041 gggcgagcag aagctggtgg tgcgactggc gcatcaggag cgcccgacgg caggcagcac
     8101 gctgtggctg catctggcgg aaaatcagct gcatcttttt gatggtgaaa caggacaacg
     8161 agtatgagta actggcctta tccccgcatc gtcgctcatc gtggcggcgg taagctggcc
     8221 ccggaaaaca ccctggcggc aatcgacgtc ggggcaaaat acggtcataa gatgatcgaa
     8281 tttgacgcga agttatcgaa agatggcgag atcttcctgc tccatgacga caatctcgaa
     8341 cgcaccagca acggctgggg cgtcgtgggt gaactgaact ggcaggattt actgcgcgtg
     8401 gatgcgggca gttggtacag caaagcgttt aaaggtgagc cgctgccgtt gctttcgcag
     8461 gtggcggaac gctgtcgcga acatgggatg atggcgaata tcgaaatcaa acccaccact
     8521 ggcactggac cattaacggg caaaatggtg gcgctggcgg cacgccaact gtgggccggt
     8581 atgacgccgc cgctgctgtc atcgtttgag attgatgctt tagaagctgc acagctggcg
     8641 gcaccggaac tgccgcgtgg tttattgctt gatgagtggc gcgacgactg gcgcgaactg
     8701 accgcgcggc tgggctgcgt ctctattcat ctcaatcata agttactcga taaagcgcga
     8761 gtgatgcagt tgaaagacgc cggattacgg attctggttt ataccgtcaa taaaccccag
     8821 cgcgcggcag agttgctgcg ctggggcgtg gattgcatct gtaccgatgc gattgacgtg
     8881 attggtccga actttacggc ccaatagttt tcaacggaat gtcaggctgc ggcgtgctgg
     8941 gctgattcag catgtcgccg tttctcctct ccggcagcat atgctgctga ttcaacgaac
     9001 tatccggatt acggttgctg tttaacatcc cgccgttggt gttgggcagc atttgctgcc
     9061 gggcgggatt tcgctccccc ggctgtgact gcaacacccg ctgagaatta ttgtttatct
     9121 ggttttctaa atgctgctgt tgcaactgag tttgcgtttt cagttgctga ttcagcatcc
     9181 ctttttgctg gatttgctga gtttgcatct gggtttgcat ccgctgctgg ctgggtatct
     9241 gataccccgg ctggttaggg ttgttcagag tattaatggg ctgtgcaaag ccgacaaacg
     9301 gcaggagtgc cgtaagaatc agaagtcgtt tcatcgcgta tcctcctctg aagatatcct
     9361 ttaagtttac tcgcttcccg acaaaacgat gattaattca gagttatata ccaggcttag
     9421 ctggggttgc cccttaatct ctggagaata acgatgataa aaccgacgtt tttacgccgg
     9481 gtagccattg ctgctctgct ctcaggaagt tgttttagcg ccgtcgccgc gcctcctgtt
     9541 tcttacggcg tggaggaaga tatcttccat ccggtacgcg cgaaacaggg gatggtggcg
     9601 tctgtcgatg ccactgccac ccaagtgggg gtagatattc taaaggaggg cgggaatgcc
     9661 gttgatgccg ccgtggcggt gggctacgcg ctggcggtaa cgcatccgca ggcagggaat
     9721 ctgggcggtg gtggttttat gctgatccgc tcgaaaaatg gcaataccac cgctatcgat
     9781 ttccgcgaga tggcacccgc taaagcgacc cgcgatatgt tccttgatga ccagggcaac
     9841 ccggacagca aaaaatcact cacttcgcat ctggcttccg gcacgcccgg tacggtcgct
     9901 ggtttctcgc tggcgttgga taagtacggc actatgccgt tgaacaaagt ggtgcagccc
     9961 gcgttcaaac tggcacgcga tggttttatc gttaacgatg ctttggcaga cgatctcaaa
    10021 acctacggta gcgaagtgtt gccgaatcac gaaaacagca aagctatctt ctggaaagag
    10081 ggcgaaccgc tgaaaaaggg cgacaagctg gtgcaggcga acctggcaaa gagcctggag
    10141 atgattgccg aaaacggccc ggacgaattc tataaaggca cgatagcgga acagattgcc
    10201 caggagatgc agaaaaacgg tggcttgatc actaaagaag atttagcggc gtataaagcg
    10261 gtcgaacgca caccgataag tggcgattat cgcgggtatc aggtttactc catgccaccg
    10321 ccatcctccg gcgggatcca tatcgtacaa atcctcaata ttctggaaaa cttcgatatg
    10381 aagaaatacg gctttggcag cgccgatgcg atgcaaatca tggcagaagc ggagaaatac
    10441 gcctacgccg accgctcgga atatcttggc gacccggatt ttgtcaaagt accgtggcag
    10501 gcgctgacca ataaagccta tgccaaatct attgccgatc aaattgatat caataaagcg
    10561 cagccatcca gcgaaattcg ccccggcaag cttgcgcctt atgagagtaa tcaaactacc
    10621 cattactcag tggtggataa agatggtaac gcggtggcgg tgacctatac gctgaacacc
    10681 accttcggta cgggcattgt cgcgggcgag agcggtattc tgcttaataa tcagatggat
    10741 gatttctccg ctaaaccggg cgtaccgaac gtttacgggc tggtgggcgg tgatgccaac
    10801 gccgtcgggc cgaataaacg tccgctgtcg tcaatgtcac caacgattgt ggtgaaagac
    10861 ggtaaaacct ggctggtcac cggtagccct ggcgggagcc ggatcatcac tacagtgctg
    10921 caaatggtgg tgaatagcat cgattatggc atgaacgtcg ccgaagcgac caatgcgccg
    10981 cgtttccacc atcagtggtt gccggacgag ctgcgtgtcg aaaaagggtt tagcccggat
    11041 acgctcaagc tgctggaagc aaaaggtcag aaagtggcgc tgaaagaggc gatgggcagt
    11101 acacaaagca ttatggttgg gccggacggt gagttgtacg gcgcatccga cccgcgctcg
    11161 gtggatgatt taacggcggg gtactaaggt tagcggccct cttcgtggga agagggctat
    11221 tttgtcaggg caagccgaag gtagcctttt ttatttcgta atcctgtaga tattcttcca
    11281 gcggctttgc cgttcccaga gaatgaattt cagcactgtc tttatcaata ataaaaggtg
    11341 cgttaccagc taagcgcgcg gcctcatctc cagtttcgag aaattctcgt gcttcgaaac
    11401 agaaatacca gccctagcta aagcgtccat gtagagtaat gacgaccggg agatctgcat
    11461 catcaaggta atggttcgct ttcgcgaatg cgtcgtgata agtaatcata attataataa
    11521 atattattgt tgagtgttat attattattc ctgtgtgata cattgagcaa agacgcgttc
    11581 atcttcgtca aagatattat taaatgctgg ttttgaaaaa agttcatcgg ataagatata
    11641 gtcacgaaat aagtatctgc taatatttta tgatttcttt ttcagaacgt cgaacgggat
    11701 tttcttattt aagatatcag aatcttatga ttcgggtaaa attctaaata aatcaacatg
    11761 tcattaaaat catcatagcc attagctatt tcggctaaaa tagagactac atgtcttcgg
    11821 tccatctcac ttaaggagtg tagttccgtt gtaagttttt ccatagcttg cactgctaaa
    11881 tttcgaacaa ggaattttct gctggtaatc tctaaaaaga tggcat
//
LOCUS       DAJTMC010000127        11892 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.127, whole genome shotgun sequence.
ACCESSION   DAJTMC010000127 DAJTMC010000000
VERSION     DAJTMC010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11892
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.127"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(48..362)
                     /locus_tag="OO130_003076"
                     /pseudo
     CDS             complement(48..362)
                     /locus_tag="OO130_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
     gene            complement(477..875)
                     /locus_tag="OO130_003077"
     CDS             complement(477..875)
                     /locus_tag="OO130_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF937 domain-containing protein"
                     /protein_id="HCR5366391.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQSNQSVSGEQLESAPGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSSQANNDLLSAGMELLKGKLFR"
     gene            complement(1115..3529)
                     /gene="gyrB"
                     /locus_tag="OO130_003078"
     CDS             complement(1115..3529)
                     /gene="gyrB"
                     /locus_tag="OO130_003078"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HCR5366392.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADLLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(3558..4631)
                     /gene="recF"
                     /locus_tag="OO130_003079"
     CDS             complement(3558..4631)
                     /gene="recF"
                     /locus_tag="OO130_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HCR5366393.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWACFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLVEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(4631..5731)
                     /gene="dnaN"
                     /locus_tag="OO130_003080"
     CDS             complement(4631..5731)
                     /gene="dnaN"
                     /locus_tag="OO130_003080"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HCR5366394.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(5736..7139)
                     /gene="dnaA"
                     /locus_tag="OO130_003081"
     CDS             complement(5736..7139)
                     /gene="dnaA"
                     /locus_tag="OO130_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HCR5366395.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            7436..7516
                     /locus_tag="OO130_003082"
     CDS             7436..7516
                     /locus_tag="OO130_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5366396.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            7746..7886
                     /gene="rpmH"
                     /locus_tag="OO130_003083"
     CDS             7746..7886
                     /gene="rpmH"
                     /locus_tag="OO130_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HCR5366397.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            7903..8262
                     /gene="rnpA"
                     /locus_tag="OO130_003084"
     CDS             7903..8262
                     /gene="rnpA"
                     /locus_tag="OO130_003084"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HCR5366398.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            8226..8483
                     /gene="yidD"
                     /locus_tag="OO130_003085"
     CDS             8226..8483
                     /gene="yidD"
                     /locus_tag="OO130_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HCR5366399.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            8486..10132
                     /gene="yidC"
                     /locus_tag="OO130_003086"
     CDS             8486..10132
                     /gene="yidC"
                     /locus_tag="OO130_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HCR5366400.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISTFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKTQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            10238..11602
                     /gene="mnmE"
                     /locus_tag="OO130_003087"
     CDS             10238..11602
                     /gene="mnmE"
                     /locus_tag="OO130_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HCR5366401.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
     gene            11845..>11892
                     /gene="tnaC"
                     /locus_tag="OO130_003088"
     CDS             11845..>11892
                     /gene="tnaC"
                     /locus_tag="OO130_003088"
                     /inference="COORDINATES: protein motif:HMM:NF019665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HCR5366402.1"
                     /translation="MNILHICVTSKWFNID"
BASE COUNT         2721 a         3255 c         3071 g         2845 t
ORIGIN      
        1 ataaggcgcg gacaggggta tcgcttagtt tttcaatttc ctatacgtga accgacttcg
       61 cgagagagtt tttccaggaa acgatagtcg tcataactga gagcagtttg cgccacggtg
      121 ctaccatcag cggccttctg taccagcgcg ccgttataag taatgcagta gtcgcccggc
      181 tgttccatat gcagctcttt caggtagttg tgaacgcctg cataagggcg acccgtcgtt
      241 agcacgacat tcacgccacg ggcgcgagct gcggcaatcg catttttaac ggcgggtgaa
      301 atggtgtgat cgggcagcag aagggtgcca tccatatcga tagcaatgag tttgatagcc
      361 atgagttccc caggtagatt agttcctgac ccatgctaac gcgattccgc tcaaaaatca
      421 gtacaacacc cgagggaaaa gggggatgca acgcgggttg catgctcccc atttgcttag
      481 cggaagagtt tccctttcag cagttccata cctgcggaaa gcagatcgtt attggcttgt
      541 gaagacactt caccttgcgg tgagagcgca tcaataatct tcggcaattg ttctgccagt
      601 aaactggaag ccgtactggt atccacgcca agtttttgcc cgagatcgga caccgcattt
      661 gtgccgggcg ccgattccag ttgctcgccg ctaactgatt gattgctctg ctgattactc
      721 aaccaggttg agagaatggc ccctaagccg ccactttgca gtttttccag cagcacctga
      781 atgccgccct gctcctcaac ccaacttaaa atagcctgat atttccccgc atcgcctttc
      841 agaaaggcac cgacaacttc atcaaaaagc cccatgataa tcacctgtaa agcgttacgt
      901 gttgacccaa aaagtataga tctgcgggat gataattgcg gattgcagaa ataaaaaggg
      961 cggagatgat ctccgccctt tttttatagc ttcttgccgg atgcggcgtt aacgtcttac
     1021 ctggcctaca aaatcattaa aattcaatac attgcaagat tttcgtaggc ctgataagcg
     1081 tagcgcatca ggcacgctcg catggttaac gccattaaat atcaatattc gccgctttca
     1141 gggcgttctc ttcaataaac gcacgacgcg gttctacggc gtcgcccatc agcgtggtga
     1201 acaacaggtc agcggcaatc gcatccttaa cggtaacgcg cagcatacga cgactttccg
     1261 ggtccatggt ggtttcccac agctgttccg ggttcatctc gcccagacct ttataacgct
     1321 ggatggagag accgcgacga gactctttca ccagccagtc cagcgcctgc tcgaagctgg
     1381 ctaccggctg acgacgctcg ccacgttcga taaatgcatc ttcttccagc aagccacgca
     1441 gtttctcacc cagcgtgcag atacgacgat attcgccacc agtgataaat tcgtgatcca
     1501 gcggatagtc agtatccaca ccgtgggtac gcacgcgaac aatcggctcg aacaggtttt
     1561 gttcggcatt ggtgtggaca tcaaacttcc actggctgcc gtgctgttct ttgtcgttca
     1621 gttcgctgac cagcgcgttc acccagcggg taacggtctg ctcatcagag aggtcggctt
     1681 ccgtcagcgt cggctgatag ataagctctt tcagcattgc tttcggataa cgacgctcca
     1741 tgcggttgat cattttctgc gtcgcgttgt actcagacac cagtttctct aacgcttcgc
     1801 cagccaatgc cggtgcgctg gcgttggtgt gcagcgttgc gccgtccagc gcgatagaga
     1861 tctggtactg atccatcgct tcgtcgtctt taatgtactg ttcctgcttg cctttcttca
     1921 ctttgtacag cggcggctga gcgatgtaga cgtgaccgcg ttcaacgatt tccggcatct
     1981 gacgatagaa gaaggtcaac agcagcgtac gaatgtgcga gccgtcgacg tccgcatcgg
     2041 tcatgatgat gatgctgtga taacgcagtt tgtccgggtt gtactcgtca cgaccgatac
     2101 cacagccaag cgcggtgata agcgtcgcca cttcctgaga agagagcatc ttatcgaagc
     2161 gcgctttctc aacgttgagg attttaccct tcagcggcag aatcgcctgg ttcttgcggt
     2221 tacgcccctg cttcgcagag ccgcccgcgg agtccccttc caccaggtac agttcggaaa
     2281 gcgccggatc gcgttcctgg cagtctgcca gtttgcccgg caggcccgct aagtcgagcg
     2341 cacctttacg gcgggtcatt tcacgcgcgc gacgcgccgc ttcacgggca cgggcagcat
     2401 cgataatttt gccaaccacg attttcgcgt cggttgggtt ttccagcagg tattctgcca
     2461 gcagttcgtt catctgctgt tcaaccgccg atttcacctc agaagaaacc agtttgtctt
     2521 tggtctggga ggagaatttc gggtccggca cttttacgga aacgaccgca atcaggcctt
     2581 cacgcgcatc gtcaccggtg gcgctgactt tggctttttt gctgtagcct tctttgtcca
     2641 tgtaggcgtt cagggtacgg gtcatcgccg cacggaagcc tgccaggtga gtaccgccgt
     2701 cacgctgcgg aatgttgttg gtaaagcagt agatgttttc ctggaagcca tcgttccact
     2761 gcaacgccac ttcgacgcca ataccgtctt tttcggtgga gaagtagaag atattcggat
     2821 ggatcggcgt tttgttcttg ttcagatatt caacgaacgc cttgatgccg ccttcatagt
     2881 ggaagtggtc ttctttgccg tcacgcttgt cgcgcagacg aatggaaacg ccggagttga
     2941 ggaacgacaa ctcacgcaga cgtttcgcca gaatttcata ttcgaactcg gtcacattgg
     3001 tgaaggtttc gaggctgggc cagaaacgca ccatggtgcc ggttttttca gtctcgccgg
     3061 taaccgccag cggagcctgc ggtacaccgt gttcgtagat ctgacggtga attttaccct
     3121 cgcgctggat aaccagctcc agtttttgcg acagggcgtt tactaccgaa acaccaacgc
     3181 cgtgcagacc gccggacact ttataggagt tatcgtcaaa tttaccgcct gcgtgcagaa
     3241 cggtcatgat cacttccgcc gccgatacgc cctcttccgg gtgaataccg gtcggaatgc
     3301 cgcgcccgtc atcctgtaca gagacagagt tatcggcgtg aatggtgacg ataatttctt
     3361 tacagtgacc cgcgagcgct tcgtcgatag cgttatctac cacctcgaat accatgtggt
     3421 gcagaccggt gccgtcatcc gtgtcgccga tatacatacc cgggcgctta cgcaccgcat
     3481 ccagcccttt caggactttg atactggagg agtcataaga attcgacatc aacgtttctc
     3541 gctcatttat acttgggtta atccgttatt ttaccctttt ccacggtaaa catcttcgaa
     3601 ttttcgtccg acatgtctat aacgtgttca gcactgatcg cgctgacaaa gacctgtgat
     3661 tgcgtcgctt ttaagcggct ggcaagcagc ccgcgacgct catcatcaag ctcagaggca
     3721 aaatcatcta tcaggtagag acaccgccgc ccgctttcac gggtgaggaa ctctccttgc
     3781 gccagacgta aggcgcacat caacagctta agctgcccac gcgataaggt atcttccacc
     3841 ggcgcaccgt cggcgcgaat gcgtaagtcc gctttatgcg ggccatgcgc ggtgtaggtt
     3901 agctggcgat cgcgttcaaa attacgttcc agcacctcag catattctgt ctctttctcc
     3961 cagccgcgct ggaaagagaa agtcagagaa aactcaggga gaaattgctt acaagtatcg
     4021 gccatatcag ccgcgatacc ggcgctatac tccgcgcgcc aggtgctgat ttgctccacc
     4081 agcgggatca gctctttatc ccacgggcgt agctgttcgt aacgtgtcac ctggcgcagc
     4141 gccgcattgc gctgcttgag caatcgcttg agattgctcc aggcggtgaa aaatccgggt
     4201 tcgttgtgaa agcatgccca gtcgagaaat gctcttctgt atttggggcc gccgttgagt
     4261 aaagtaaacc cttctggcgt tatcagctgc atcggcatca ggtgcgccag ctccgcgact
     4321 ttatgcccgt cagtaccgtc gatgcggact ttgctgtcgc cttgtttgtc tttggttaag
     4381 ccaatcgccg tctcgcgctc ttcgccctgt aatcgcccat ggagaacaaa tgcctcctgc
     4441 tcatggcgaa tgacgcgacc aatctgcaaa ctgcgaaacg cccgaccatg gccgagcgta
     4501 tagatggctt ccagcacact ggttttgcca ctgccgttgg cacctaccag aaagttaaag
     4561 ccgggagata aggcgagatc cgcggtttcg atattgcgga aatcgcggat caacaagcgg
     4621 gtgagggaca ttacagtctc attggcatga caacataagc cgcgctctgg ctggccgcat
     4681 cttcaatctg cacgctggaa accgaatcgg tcagcatcat gcggacgttt tcgcatttca
     4741 gcgcgttcag aacatccagc acatagctga cgttgaagcc gatttccatc tccgcaccgc
     4801 tataggtaac gtcgaggatc tcttccgctt cttcctgttc cgggttgttg gcagtgattt
     4861 tcagctggtt ttcgctgaca taaagacgta cgccgcggaa tttctcgtta gagagaattg
     4921 ccgcacgggc aaacgcctgc ttgagcagat cgcagccagc ttccagatgt ttgtccgggt
     4981 tcttcggtag aacgcggcgg taatccggga agcgaccatc caccagtttg gaggtgaaga
     5041 taaagtcgcc aacgtgggca cgaatgttat tgctgccaat ctgcacgcgc agcggattgt
     5101 cgccgccgtc gagcatacgc atcagttcaa tcacgccttt acgcggcacg atcaccgaat
     5161 ggcttggcaa agattgacca attggcattg aacagactgc cagacggtga ccatcggtcg
     5221 ctacggtgcg cagttcttcg ccttcggttt caaacagcat accgtttaag taatagcgaa
     5281 catcctgatg cgccatcgaa aactgggtcg cttcaatcag acgcttcatc gtcgcctgcg
     5341 gcagggtaaa ttcgacttcg ctctgccagt catcgaggtt cgggaaatcc gccgctggca
     5401 gggtagacag cgaaaaacgg ctacgcccgg agcgcaccag catccgttca ccttccagct
     5461 gcacggcaat ttccgcgcct tcaggcagac cacggcagat atcaaagaat ttgcgcgccg
     5521 gaacggtcgt tgctcctggc tcgtgtggct gaaccagcgc aacacgtgcc accatttcca
     5581 tctcgagatc agtaccggtc agcgacaacg taccgtcagc aacctgtaac agcagattac
     5641 cgagaatcgg tagcgtagga cgaccaccta acggaccgct cacctgttgt agcggtttta
     5701 ataaatgctc acgttctacg gtaaatttca taggtttacg atgacaatgt tctgattaaa
     5761 tttgaaaaat cttctttgat atcgtggctc tcttcacgca actgctcgat cttacggcag
     5821 gcatgaagca ccgtcgtgtg gtcacgacca ccaaacgcat cgccaatctc cggcagactg
     5881 tggttagtca gctctttcgc cagcgccatc gccatctggc gcggacgcgc caccgagcgg
     5941 gatcgacgct tggaaaggag atccgcgact ttgatcttgt agtactccgc caccgtcttc
     6001 tgaatattgt cgatggtgac cagtttttcc tgcaatgcca gcaagtcgcg cagcgcctca
     6061 cgcacgaagt cgatggtgat cgcccgtccg gtaaagttgg cattggcaat gacgcggttc
     6121 agcgcccctt ccagctcacg tacgttagat cgtagacgct tggcgataaa gaacgccact
     6181 tcgcccggca aacgaatgtc gttttcgtcg gcctttttca tcaggatcgc cacacgggtt
     6241 tccagctctg gcggttcgat cgccacagtc agtccccaac cgaagcggga tttcaaacga
     6301 tcctcaacgc cgttgatctc tttcggatag cgatccgagg tgagaatgat ctgttgatta
     6361 ccttccagca gggcgttgaa ggtatggaaa aactcttcct gagatcgttc tttattagca
     6421 aaaaactgaa tatcgtcgat cagcagtgca tctacggaac ggtagtagcg tttaaactct
     6481 tcgatcgcgt tgttttgcag ggctttaacc atgtcctgaa caaagcgctc ggagtgcata
     6541 taaaccactt tggcattcgg cttgcgcgcc ataatgccgt tacccaccgc atgcagtagg
     6601 tgagttttac ccagacccgt gccgccataa aggaacaacg ggttataggc accgccaggg
     6661 ttatccgcca cctggcgagc cgccgcgcgc gccagttggt tagatttacc ttcaacgaag
     6721 ttatcaaacg tgtgtttgac gtttacgtta gaacgatagg tcggttctgc cggggccggg
     6781 acgttatccc aacctgagcg cgtagaaggc gcagcacgtt gcggctgcgt ttgcgccacc
     6841 tgtgcagggg ccgcgacgtt gctcgtcact gccgcttgtg gcgtttgcgt caccggtttg
     6901 gtgccgactt caaaacgcag ctgtggggca tccgctccgc agaaactggt tagcagtcca
     6961 ttgatattat taaggtactt gtcccgtacc caatcgagga caaaacggtt tggcgcgtac
     7021 agggccagcg tgttatcgct cagttccgcc tgcaatgggc gtatccacat actgaattct
     7081 gtggctggta actcatcctg caatcgggca agacactgct gccaaagcga aagtgacacg
     7141 gcggactcca ctcgaacaaa agtcgataat gactaaggct gaaacattca tgattgttga
     7201 cgtacgtcga aaagaccctg cttgagggtg acgcacgaac cgctgtctgc ggttatatgc
     7261 ccgatcaaga tcctgcaaaa cgatcgggac cgcggatcat agcctaaact gcgcaagaga
     7321 tcttctgttt ctcacagatt ttcccgattt atccacagga ctttccagaa ctcgctaagt
     7381 gtaaacgatc ctgccgcgag gcgggcacga tttacgccac atattgaaaa atttaatgac
     7441 catagacaaa aattggctta atcgatctaa taaagatcca ggacgatcct tgcgctttac
     7501 ccatcagccc gtataatcct ccacccggcg cgccatgctg gtttccactg gtgtgaggtc
     7561 gtacattttc cctgcgaaaa ggtgcggaaa agcgcggtaa ataaggaaag agaattgact
     7621 ccggagtgta caattattac aatccggcct ctttaatcac ccatggcttc ggtgtccatc
     7681 gtttcatttt tcggcggata tccaataaag ccattgaatt tattcaagtt taggtagaaa
     7741 tcgccatgaa acgcactttt caaccgtctg tactgaagcg caaccgttct cacggcttcc
     7801 gtgctcgtat ggctactaaa aatggtcgtc aggttctggc acgtcgtcgt gctaaaggcc
     7861 gcgctcgtct gaccgtttct aagtaatcaa gctaacccct gagtggttaa gctcgcattt
     7921 cccagggagt tacgcttgtt aactcccagt caattcacat tcgtcttcca gcagccacaa
     7981 cgggctggca cgccgcaaat taccattctc ggccgcctga attcgctggg gcatccccgt
     8041 atcggtctta cagtcgccaa gaaaaacgtt cgacgcgccc atgaacgcaa tcggattaaa
     8101 cgtctgacgc gtgaaagctt ccgtctgcgc caacatgaac tcccggctat ggatttcgtg
     8161 gtggtggcga aaaaaggggt tgccgacctc gataaccgtg ctctctcgga agcgttggaa
     8221 aaattatggc gccgccactg tcgcctggct cgcgggtcct gatagccctc attcgggtct
     8281 atcaacgcct gattagtccg ctactcgggc cgcattgtcg tttcactcca acctgttcaa
     8341 gctacggaat tgaggcattg cgcaggtttg gagtgataaa aggcagttgg ttgacggtga
     8401 aacgcgtatt aaaatgccac cctttacacc ctggtggtga cgatcccgtc ccgcccggac
     8461 catttgatac cagagaacac taacgatgga ttcgcaacgc aatcttttag tcatcgcttt
     8521 gctgttcgtg tctttcatga tctggcaagc ctgggagcag gataaaaacc cgcaacctca
     8581 ggcccaacag accacgcaga caacgaccac cgcagcgggt agcgccgccg accagggcgt
     8641 accggccagt ggccagggga aactgatctc ggttaagacc gacgtgcttg atctgaccat
     8701 caacacccgt ggtggtgatg ttgagcaagc tctgctgcct gcttacccga aagagctgaa
     8761 ctctacccag ccgttccagc tgctggaaac ttcaccgcag tttatttatc aggcacagag
     8821 cggtctgacc ggtcgtgatg gcccggataa cccggctaac ggcccgcgtc cgctgtataa
     8881 cgttgaaaaa gacgcttatg tgctggctga aggtcaaaac gaactgcagg tgccgatgac
     8941 gtataccgac gcggcaggca acacgtttac caaaacgttt gtcctgaaac gtggtgatta
     9001 cgctgtcaac gtcaactaca acgtgcagaa cgctggcgag aaaccgctgg aaatctccac
     9061 ctttggtcag ttgaagcaat ccatcactct gccaccgcat ctcgataccg gaagcagcaa
     9121 cttcgcactg cacaccttcc gcggcgcggc gtactccacg cctgacgaga agtacgagaa
     9181 atacaagttc gataccattg ccgataacga aaacctgaac atctcttcga aaggtggttg
     9241 ggtggcaatg ctgcaacagt atttcgcgac ggcgtggatc ccgcataacg acggtaccaa
     9301 caacttctat accgctaatc tgggtaacgg catcgccgct atcggctata aatctcagcc
     9361 ggtactggtt cagcctggtc agactggcgc gatgaacagc accctgtggg ttggcccgga
     9421 aatccaggac aaaatggcag ctgttgctcc gcacctggat ctgaccgttg attacggttg
     9481 gttgtggttc atctctcagc cgctgttcaa actgctgaaa tggatccata gctttgtggg
     9541 taactggggc ttctccatta tcatcatcac ctttatcgtt cgtggcatca tgtacccgct
     9601 gaccaaaacg cagtacacct ccatggcgaa gatgcgtatg ctgcagccga agattcaggc
     9661 aatgcgtgag cgtctgggcg atgacaaaca gcgtatcagc caggaaatga tggcgctgta
     9721 caaagctgag aaggttaacc cgctgggcgg ctgcttcccg ctgctgatcc agatgccaat
     9781 cttcctggcg ttgtactaca tgctgatggg ttccgttgaa ctgcgtcagg caccgtttgc
     9841 actgtggatc cacgacctgt cggcacagga cccgtactac atcctgccga tcctgatggg
     9901 cgtaacgatg ttcttcattc agaagatgtc gccgaccaca gtgaccgacc cgatgcagca
     9961 gaagatcatg acctttatgc cggtcatctt caccgtgttc ttcctgtggt tcccgtcagg
    10021 tctggtgctg tactatatcg tcagcaacct ggtaaccatt attcagcagc agctgattta
    10081 ccgtggtctg gaaaaacgtg gcctgcatag ccgcgagaag aaaaaatcct gattcggtga
    10141 gttttcgcta aaataagggc ggtcagttga ccgccttttt tcttttcgta gggcggataa
    10201 gcaccgcgca tccgccacac aaagcaacag gaacatcatg agcgataatg acactatcgt
    10261 agcccaggcc acgcctccgg gacgtggcgg cgttggcatc ctgcgcatct ccggcttcaa
    10321 agcccgtgaa gttgccgaaa ccgtgctggg taaactgcct aagccgcgct acgccgatta
    10381 tcttccgttt aaagacgccg acggcagcgt actcgatcag gggattgcgc tatggttccc
    10441 tggcccgaac tcgttcaccg gcgaagatgt gctggaactg caaggtcatg gcggtccggt
    10501 gatcctcgac ctgctgttaa aacgtattct gaccattccc ggcctgcgga ttgctcgccc
    10561 tggtgagttt tccgaacgcg cgtttcttaa cgataaactt gacttagccc aggccgaggc
    10621 gattgccgat cttatcgacg ccagttcgga acaggcggcc cgttcggcac ttaactcgct
    10681 gcaaggcgca ttctccgcac gggttaatca tctggtagaa gccctcaccc acttgcgcat
    10741 ttacgtcgaa gcggcaattg atttccccga tgaagagatc gatttcctct ccgacggaaa
    10801 aattgaagcc cagctcaatg acgttattgc cgatcttgat gcagtgcgtg ctgaagcacg
    10861 tcagggtagt ttgttgcgcg aagggatgaa agtggtgatt gccggacgtc ctaacgccgg
    10921 taaatcgagc ctgttaaacg cgctggcggg gcgtgaagcg gcaatcgtaa ccgatatcgc
    10981 cggaactacg cgtgacgtgc tgcgtgagca tatccacatt gacggaatgc cgctgcatat
    11041 catcgatacc gccgggctac gtgaagccag tgacgaagta gaacgtattg gtatcgagcg
    11101 cgcgtggcag gaaattgaac aggccgaccg cgtgctgttt atggtcgatg gcaccacaac
    11161 agacgccgtg gatccggcag agatctggcc ggaatttatt gcccgtctgc cagcgaaact
    11221 gccgatcacc gtggtgcgca ataaagccga tatcaccggc gaaacgctgg gaatgagtga
    11281 agtgaacggt cacgcgttaa ttcgtctctc ggcaaggact ggtgaaggcg tggacgtgct
    11341 gcgtaaccat ctcaaacaga gcatgggctt tgacaccaac atggaaggcg gcttcctggc
    11401 gcgtcgtcgc cacctacagg cgctggaaca ggcagcggaa catctacaac agggcaaagc
    11461 gcaactgttg ggagcctggg caggtgaact gctggcggaa gagttgcgtc tggcacagca
    11521 gaacttaagc gaaatcaccg gggaatttac ttcagacgac ctgctggggc ggattttctc
    11581 cagcttctgt attggtaagt aaccgcgctt acgaagccgt attctgactg tcagatgcgg
    11641 cttcgcttca ttgttagcac tcctgttatt cctcaaccct ttttttaaac attaaaattc
    11701 ttacgtaatt tataatctta aaaaaagcat ttaatattgc tccccgaacg attgtgattc
    11761 gattcacatt taaacaattt tagaatagac aaaattcttg agtattatac tgtagcctcg
    11821 tgtctttcga ggaataagtg cattatgaat atcttacata tatgtgtgac ctcaaaatgg
    11881 ttcaatatcg ac
//
LOCUS       DAJTMC010000128        11793 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.128, whole genome shotgun sequence.
ACCESSION   DAJTMC010000128 DAJTMC010000000
VERSION     DAJTMC010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11793
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.128"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            485..1141
                     /gene="pphA"
                     /locus_tag="OO130_003089"
     CDS             485..1141
                     /gene="pphA"
                     /locus_tag="OO130_003089"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HCR5366403.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVCAVRGNHEQMAMDALASQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
                     VLWSRSRLGERQKGQGITGADHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTLVQLQ
                     "
     gene            complement(1142..1333)
                     /locus_tag="OO130_003090"
     CDS             complement(1142..1333)
                     /locus_tag="OO130_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HCR5366404.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRQGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(1438..1674)
                     /locus_tag="OO130_003091"
     CDS             complement(1438..1674)
                     /locus_tag="OO130_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HCR5366405.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            complement(1792..3231)
                     /gene="rsmF"
                     /locus_tag="OO130_003092"
     CDS             complement(1792..3231)
                     /gene="rsmF"
                     /locus_tag="OO130_003092"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HCR5366406.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNQEENEAVCLWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKD
                     REAGQIRQAAAGVGLNWDENLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALATPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTGNA"
     gene            complement(3311..5944)
                     /gene="yebT"
                     /locus_tag="OO130_003093"
     CDS             complement(3311..5944)
                     /gene="yebT"
                     /locus_tag="OO130_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HCR5366407.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RSNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGTGKEQDHFVALDTQPKYRLNNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVDPSVVTL
                     LRENTRIELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLHEPDV
                     LTLTLTAPESYGIDTGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVSGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISATGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            complement(5913..7196)
                     /gene="yebS"
                     /locus_tag="OO130_003094"
     CDS             complement(5913..7196)
                     /gene="yebS"
                     /locus_tag="OO130_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HCR5366408.1"
                     /translation="MALNTPQITPTKKITVRSIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDTITGAMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERYY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            7326..7823
                     /gene="msrC"
                     /locus_tag="OO130_003095"
     CDS             7326..7823
                     /gene="msrC"
                     /locus_tag="OO130_003095"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HCR5366409.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            7920..8618
                     /gene="proQ"
                     /locus_tag="OO130_003096"
     CDS             7920..8618
                     /gene="proQ"
                     /locus_tag="OO130_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HCR5366410.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            8638..10686
                     /gene="prc"
                     /locus_tag="OO130_003097"
     CDS             8638..10686
                     /gene="prc"
                     /locus_tag="OO130_003097"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HCR5366411.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FGPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            10878..11759
                     /gene="htpX"
                     /locus_tag="OO130_003098"
     CDS             10878..11759
                     /gene="htpX"
                     /locus_tag="OO130_003098"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HCR5366412.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
BASE COUNT         3033 a         2906 c         3043 g         2811 t
ORIGIN      
        1 caaccccaaa tcgtaaagga aaccgtagca cgtcgtatgc aagaacgtgc cacggctggc
       61 tgatggatgt tcgatagcgc gagtttgaat gaaaatcagc cggagatgat tttacataat
      121 tgctacggaa ttattcaata caggaattgc ttgcgtctgc atggattgac ctgaaatttt
      181 cccgaaaatt tctctaaaaa actcgaaaaa aatggtaact ggttgaatgt attaatatgc
      241 aatggtacgt gtcagggatt aaaagatgaa cgtaaattta ttcaacgcat taattttaaa
      301 gggttttatt gtttgttgac gaaaacagga atcgtgttcg gtctcttttt atctgttaaa
      361 agccagaagc atttccttcg ctgactttat agtcaaccat aacacacact ctactgtctg
      421 agtccagcgt tttttaacat tcttgttaag attatgtgat ctttagcacg ggaggaaaat
      481 attgatgaaa cagcctgcgc ccgtttatca gagaattgcg ggtcatcaat ggcgacatat
      541 ctggctttct ggcgatatac acggttgtct tgagcagttg cgccgcaaat tatggcattg
      601 tcgttttgat ccgtggcgag atttacttat ctcagtggga gacgttatcg atcgtgggcc
      661 gcaaagttta cgttgtctgc agttactgga acaacattgg gtttgtgcgg taagaggcaa
      721 tcatgaacag atggcgatgg atgcgctggc atcccagcag atgtctttgt ggttgatgaa
      781 tggcggcgac tggtttattg cgctggcaga taatcaacag aaacaagcga aaacggcgct
      841 ggaaaaatgt cagcatttgc cctttattct tgaagtacac agtcgcaccg gcaagcatgt
      901 tattgctcat gcggattatc cagatgatgt ttatgaatgg caaaaggacg ttgatttgca
      961 tcaggtcttg tggagccgct cgcgattagg tgaacgccaa aaagggcagg gaattacagg
     1021 tgctgatcat ttctggtttg gtcatacacc gttgcgacat cgcgtggata ttggcaacct
     1081 gcattatatt gataccggtg ctgtctttgg gggcgaactg actcttgtgc aattgcaatg
     1141 attaaaaatc accgtactcc tgtgcaggtc gccagaaacc atctataaaa tcctcaatcg
     1201 gaaaacaacc gccctggcgg atccgttgat cgctcataga ataaagacac tgctgttccg
     1261 tgttgtagac atccacaaca atatcttcac aaccgccatc caggtagcaa acaaaaagta
     1321 ccagcgcgaa catttcatcc ccgaagtgtg gtgccgtacc gttaagttta ggagagattt
     1381 tacaacgggg gaataaccag gacaaataac ccgccatgcc gacgggttct ttttggatca
     1441 ggcaagacgc ataatccagg catcagattg ttgatcgtaa cgggtacggt aaaggacaga
     1501 gtgttcgcca tttagcaggg cagggatact ggtttcagca tcccaggcat cgagttgcat
     1561 acataaatcg gggtcagatt tacaaggaat ggttaacgtt cgatcacccg gcgattcacc
     1621 gtgtaattcg ccgtcgtcga tttcaaaagc gcctatgcgc acactggttt tcatcttcgt
     1681 gctctccatt tagctgccgg ttgtggtacg accagtccga tcgcaccatt tttcagccta
     1741 gttgaggcag ccgaatgtgc cagtcaaaaa gtgcgctttt tttgtgcgct gtcaggcgtt
     1801 accggtaaaa agtttcccat cgcgcaccag ttcacgcgga tagctgtttt tcaatcgcga
     1861 accaatccgt ttggctaaac caatcggttg atgctggaaa gtaaccaaca cgtcatccgc
     1921 cactggcgcg gcttgcgggt aaacatcgcg cccgcgatac cactcctccg cttcctgcgg
     1981 tgtcagttca aaagcgttca cattgtcggg ggtggcaagg gcaataactg cttcatgctg
     2041 ccagcgataa cctttgttgt gcgtttcggc aagtttaatc cccaagcgag aaaatcggac
     2101 tttaccgatc agggcttcaa tgcccaccgg gaacaaccac agttctttgt cacgctgcca
     2161 gaggcgcagg ttttcatccc agtttaagcc aacacctgca gccgcctggc gaatttgtcc
     2221 agcttcgcga tctttcaccg ggctgaacgg aaaattaccg actttgtatt tgggggcggg
     2281 taaggcggga atcgcctgag ttttacgcag acgagcaacg aagaagcctt cgcagtcgta
     2341 aatttgtggg aaaacatgca aaaagccttc ttcggtcagc gctttgtttg caccagggaa
     2401 gagatcgcca agtggtaaaa actctactgc gtcggggtaa gtctctttca gccacaggca
     2461 aacggcttcg ttttcttcct ggtttaaggt acaggtcgag taaaccagcg taccaccagg
     2521 acgtaatgca tgaaaggcgc tgtcgataag ctcccgttgt gtagctgcga tttcctgatt
     2581 gctttctggt gaccagtttt ttagcgcatc gggatcttta cgcaccacgc cttcgccgga
     2641 gcagggagcg tccagcaaaa tggcatcaaa catttctggc actgccgcac caaacacgcg
     2701 gccatcaaaa tgtgtgagcg caacattact gatgccacag cggctgatat tggcatgtaa
     2761 cacttttacc cgactggcgg aaaactcatt ggcaaggatt gccccttcgt tattcatccg
     2821 cgcggcaatt tgcgtcgttt ttgagcctgg cgcagcagcg acatccatca cccgctgtgg
     2881 tgcattaccg tcagcaaaca aggcggcgac gggcaacatt gaactggctt cctgaatata
     2941 aaacaggcca cttaaatgct cggcggtact acccaatggc aatgcatctt cattgtcgcg
     3001 ttcaatccag aaaccttctt cacaccacgg aattggcgta agcgtccagc cataaggagc
     3061 ggttaattgc aggaaatcag caacggagat tttcagcgta ttaacgcgaa tgctgcggcg
     3121 caacgggcgc tgacaggcgg caagaaaatc atcaaatgag agcgtcgaag gcatagcttc
     3181 gcgcatttgt gtcagaaacg cgtccgggaa ataaacggtg tgttgggcca cgagcatgta
     3241 ccactggcaa aaaaacaggc gcgcagttta gcataaacgc tccggcgcag gcacgccgga
     3301 gcagtgggca ttatttggga agcgcagttc cccattcacg ccactctttc ggttcacttt
     3361 cctgcaacag gaagtgtttg ccttcctggg ctttcggtgc cagtggcgta cccggaggcg
     3421 tggcgaaggc gatgccgcca cggataaact ggttaaaggt gccggttttc actacgccgc
     3481 ccgtcagacc aaagtccaga ctgtaacccg atgccaacca gaagacggaa ttgttacgca
     3541 ccaggtgttg atagcgttta ctgatgcgca tcgcaatcat cacgcgatct gacaatgtcc
     3601 ccagcgtcat tccggtaacc gtacctactt ccagaccacg gaacagcaca ggcgtaccaa
     3661 tacctaacga accggcttcc ggtgcttcga caataatgct taagccatcc aggtaacgcg
     3721 aatcagtaat agtagcttct tgcaactcaa agtcacggcg aggattaccc cgacctggtt
     3781 cgacattgat atacggctgg aggatagtat ctagatgctc aacgcccgtg gctgaaattt
     3841 gcggtgtgac caccgagaag cgcgtaccgc cgcgggcgaa ggtctggaca tactccggat
     3901 agagcaccgc ctttgcctgt acttcattgc gcgcggtaat cagatccagc gtctggattt
     3961 gcccgatatc aataccgaga tagcgaattg gcatcccgac cgccagtttt ccggcatcga
     4021 aagcgtgaag cgtaatctgc ccactaaccg cacgggccgc tgtttcggaa gcatacagaa
     4081 ttcgtttgtc gcctttacgc tgactggcgc tggcaccgct gaggttatcg aagctaatgg
     4141 ctccctttaa tgctctggag agcggggatg cctgtacggt caggccacta ccattcagct
     4201 gaactttcgc cccgccttct gcccagaaca cgctattgct ggtcagaagg ttgcgatact
     4261 ccggcttaat atgcagatcg atatcaaacg cgttagctcg cggacgcacg gtaatcactt
     4321 caccaacttc aaatttacgg tacagcacta ccgatcctgc ctgcacatcc ggcagcgtct
     4381 ctgcactcaa actcacggtt gtggtgggta aatcgctaag gctgttctcc agcgcttttt
     4441 ccagattggc atacagtgga tagctggctt tcatctcacc tttatcgcct ggcagaatac
     4501 gtatcccgcc gttaatccat tctgaggcgc tggcaccaag aaactcaacg ccatccagcc
     4561 ccaccttcac gtcgacacgg ctgttgacga caaatttgct atcgcctttt accagttcgc
     4621 gatgctgagg ctcgatggcg acggtaaagg taacgccttt gctggtgagt ttacgatcaa
     4681 taacctggcc tacctgcacg ccgtgaagaa tgagcggctg acccgtatca ataccgtaac
     4741 tttccggcgc ggtcagggtc agcgtcagaa catcaggttc atgcagcagt gctttttcgc
     4801 ctggcacaac aacgaactct ttgcgtggct cgccatcgcc gggtaccaac tcgaaggttt
     4861 tgccagtcag cagggcgctg agattggcat cgctaaggga taatttcgga ttacgtaatt
     4921 cgatgcgggt attttcacga agcagggtaa cgacgctggg atcaactgtc atttccccag
     4981 tgactttacc accaggattt aaatccagtt tagttagctg tccgacttcc agtccctgat
     5041 acattaatgg cgtcgagtcg gcggttaatc cggccccact cggcagttcc agtttgatta
     5101 ttacgccacg ctggctatgg gcgaggtctt catacagacc aaaggtatct tccgcctcgg
     5161 caggtttcga ctcttctggt gaatcgaagg caatcgcacc gttaaccagt gccgccaggc
     5221 tttccagttt caccttcgcg ccactgatac tgacattggc atcaacgccg gaaacgttcc
     5281 agaaacggct accttttttc accagatcgg taaaacgccg ctcgatcagg acatcaatca
     5341 ccacgccttg tttgttggga ttgatggcat agtcgtagac ttttcccacc gggatcttgc
     5401 ggaaatagac caatgaaccg ctgttcagcg aaccgagatc gggggcttgc aggtggatca
     5461 tcagatcgcc attgttcagc cgatatttcg gttgggtatc gagtgcgaca aagtgatcct
     5521 gctctttacc tgtacccggc atcatgccga tatagttccc accgacgagg gcgtccagcc
     5581 cggagacacc tgccaacgat gcttttggcg tcaccagcca gaattgagtc tcttcgcgca
     5641 gcgcatcttt catatcggac ttgatgctga ccttgacttc aatcttacga agatcgtcgc
     5701 tgaggctgat atcctgcact gttccgactt caacgccctg ataacgaaca ggcgtacggc
     5761 ccgggacaat accatccgcc gacataaagt cgatggtgac ggtattactt cggtcctgat
     5821 aactgtccca aatcagccaa ccggcaatca ttagcgcgat gaaaggcagc agccagaaag
     5881 gtgagatacg gcgtttattt ttaatctgcg cttcagtcgt cgaagcgggc gtttcctgac
     5941 tcatgtgcat cccaaagtaa gcggctgtcc agccattcca cagcaagaat agtcaaaatt
     6001 acggctgcgc caaaataaaa cgcagccggt cccatagtaa aagcgaggat ctgatcgcga
     6061 ttaatcagcg acatggttaa agatatgaca aacaggtcga gcatcgacca gcgtccaatc
     6121 caggtcacca tccgcagtaa cagaatgcgt gtgcgtaaac cttgctggca tttaaaatga
     6181 atgctcaaca gtaaagtgaa catgacgatc actttagtaa acggtaccag aatactggcg
     6241 ataaacacga ttccggcgac cgcaatgttg ctacttgcca gcgacataat cccggaaaga
     6301 atcgtatctt cctgccggcc accattcaga taaatgatag aaataggcaa taggttggct
     6361 ggtaacaaca aaacgattga cgctaacagc gccgcccagc atttttgcaa actatgacga
     6421 cgacgcaggc gtagcgggat atggcaacgc gggcagcgac cacgctgatc aggatagccg
     6481 gtaaaatggc acccaagaca gacacgaagt ttctcgtccc tacgcgtagc ggggcgctgc
     6541 ggataatatc gctcccacag ctcctcaaca ttaagatgtg acaacgtcac cgtcgttaaa
     6601 atcaccaacg ccacaaaaga gaacaagccg acacccgcct ggatatgggc ataatcctgt
     6661 acctttatag aagcaacgcc aatgccgacc aggtagatat ccagcattac ccactcttta
     6721 agacgctcaa gcatcagcag taccggacgt aaattcattc ccagtcggtt accaaaccat
     6781 aaatatgcta tggaggacac cagaatgagg ggggcaccaa taacgcagaa aaagaccatc
     6841 gcccccgtta tcgtatcgcc ctgtttggtc atttgccaga tgccttgcat gacgtttgcg
     6901 tcgatacgaa tacctaacag ccagatatgc aacagcggtt cgccccaggc aaacggcatc
     6961 agcaacagca tggtgaaagc cattgccgcc aggcgcgtta gcgaccagtc gcgcccgtcg
     7021 cgaatttttg cctgacagcg cggacaatag gcactctgat gagaatttat ctcgggcagg
     7081 ctaaacagca tgtcacattg cgggcaacgt tggtaatcac cacgcggcag ttcctcgccg
     7141 attgacctca ctgttatctt ttttgtcggc gtaatttgtg gtgtgttaag agccatgacg
     7201 tgccaaatgt aataagaatt aacattaatc ttaacccatg atgggaatga tcttgagcat
     7261 taacgcattt aatgcttatt ttaataggct ggcagttcca ggtaagacaa acaagtgatt
     7321 atataatgaa caaaacagaa ttttacgcgg atttaaatcg cgactttaac gcgctgatgg
     7381 cgggagaaac cagttttctg gcaacgcttg cgaacaccag tgcgttgtta tacgagcgtc
     7441 tcactgacgt aaactgggca ggtttttatt tgcttgagga cgatacactg gtactcggac
     7501 catttcaggg caaaattgcc tgtgtccgga tacccgtcgg gcgcggggtg tgcggcactg
     7561 cggttgcccg caatcaagtg cagcgtatcg aggatgttca cgcgtttgac gggcatattg
     7621 cctgtgatgc ggcgagtaat tctgaaattg ttctgccgct ggtggtgaaa aatcagatta
     7681 ttggtgttct tgacatcgat agtaccgtct tcggtcgctt tacagacgag gacgagcaag
     7741 gcttacgtca gcttgtggca cagcttgaaa aagtgcttgc aacgacggat tacaaaaaat
     7801 tctttgcgag cgtcgcagga taatcaacgg ataacgtagc atttactggt agcgtcatta
     7861 taatgacgcc tgttcatgcc tgcgcttgtt ggctacgtcc gttgtaatca ggaaatttca
     7921 tggaaaatca acctaagttg aatagcagta aagaagtaat cgcgtttctg gccgaacgtt
     7981 ttccccactg tttcagtgcg gaaggtgaag cgcgtccgct gaaaatcggt atttttcagg
     8041 atttggtcga tcgtgttgcc ggggaaatga acctgagcaa aacgcaatta cgatccgctt
     8101 tacgtctcta cacttcgagc tggcgttatc tttacggtgt taaacccggc gcaacgcgtg
     8161 tcgatcttga cggcaaccca tgcggtgagc tggacgagca acatgtagag catgctcgca
     8221 agcagcttga agaagcgaaa gcgcgtgttc aggcacagcg tgctgaacag caagcgaaaa
     8281 aacgcgaagc tgccgcagct gctggtgaga aagaagacgc accgcgccgc gaacgcaagc
     8341 cacgtccgac cacgccacgc cgcaaagaag gcgctgaacg taaacctcgt gcgcaaaagc
     8401 cggtagagaa agcgccaaaa acagtaaaag cacctcgcga agaacagcac accccggttt
     8461 ctgacatttc agctctgact gtcggacaag ctctgaaggt gaaagcgggt caaaacgcga
     8521 tggatgccac cgtattagaa atcaccaaag acggcgtccg cgtccagctg aattcgggta
     8581 tgtctttgat tgtgcgcgca gaacacctgg tgttctgaaa cggaggccgg gccaggcatg
     8641 aacatgtttt ttaggcttac cgcgttagct ggcctgcttg caatagcagg ccagaccttc
     8701 gctgtagaag atatcacgcg tgctgatcaa attccggtat taaaggaaga gacgcagcat
     8761 gcgacggtaa gtgagcgcgt aacgtcgcgc ttcacccgtt ctcattatcg ccagttcgac
     8821 ctcgatcagg cattttcggc caaaatcttt gaccgctacc tgaatctgct cgattacagc
     8881 cacaacgtgc tgctggcaag cgatgttgaa cagttcgcga aaaagaaaac cgagttaggc
     8941 gatgaactgc gttcaggcaa actcgacgtt ttctacgatc tctacaatct ggcgcaaaag
     9001 cgccgttttg agcgttacca gtacgctttg tcggtactgg aaaagccgat ggatttcacc
     9061 ggcaacgaca cttataacct tgaccgcagc aaagcgccct ggccgaaaaa cgaggctgag
     9121 ttgaacgcgc tgtgggacag taaagtcaaa ttcgacgagt taagcctgaa gctggcagga
     9181 aaaacggata aagaaattcg tgaaaccctg acacgccgct acaaatttgc cattcgtcgt
     9241 ctggcgcaaa ccaacagcga agatgttttc tcgctggcaa tgacggcgtt tgcgcgtgaa
     9301 atcgacccgc ataccaacta tctttccccg cgtaataccg aacagttcaa cactgaaatg
     9361 agtttgtcgc tggaaggtat tggcgcagtg ctgcaaatgg atgatgacta caccgttatc
     9421 aattcgatgg tggcaggtgg tccggcagcg aagagtaaag ctatcagcgt tggtgacaaa
     9481 attgtcggtg ttggtcaaac aggcaagccg atggttgacg tgattggctg gcgtcttgat
     9541 gatgtggttg ccttaattaa agggccgaag ggcagtaaag ttcgtctgga aattttacct
     9601 gctggtaaag ggaccaagac ccgcactgta acattgaccc gtgaacgtat tcgtctcgaa
     9661 gaccgcgcgg ttaaaatgtc ggtgaagacc gtcggtaaag agaaagtcgg cgtgctggat
     9721 attccgggct tctatgtggg tttgacagac gatgtcaaag ttcaactgca gaaactggaa
     9781 aaacagaatg tcagcagtgt gatcatcgac ctgcgtagca atggcggtgg ggcgttgact
     9841 gaagcggtat cgctctcagg tctgtttatt cctgcgggtc ccattgttca ggtccgcgat
     9901 aacaacggca aggttcgtga agacagcgat accgacgggc aggtgttcta taaaggcccg
     9961 ctggtggtac tggttgaccg tttcagtgct tcggcttcag aaatctttgc cgcggcaatg
    10021 caggattacg gtcgtgcgct ggttgtgggt gaaccgacgt ttggtaaagg caccgttcag
    10081 caataccgtt cattgaaccg tatttacgat cagatgttac gtcctgaatg gccagcgctg
    10141 ggttctgtgc agtacacgat ccagaaattc tatcgcgtta acggcggcag tacgcaacgt
    10201 aaaggcgtaa cgccagacat catcatgccg acgggtaatg aagaaacgga aacgggtgag
    10261 aaattcgaag ataacgcgct gccgtgggat agcattgatg ccgcgactta tgtgaaatca
    10321 ggcgatttaa cggcctttgg gccggagctg ctgaaggaac ataatgcgcg tatcgcgaaa
    10381 gatcctgagt tccagaacat catgaaggat atcgcgcgct tcaacgctat gaaggacaag
    10441 cgcaatatcg tttctctgaa ctacgctgtg cgtgagaaag agaataatga agatgatgcg
    10501 acgcgtctgg cgcgtttgaa cgaacgcttt aaacgcgaag gtaaaccgga gttgaagaag
    10561 ctggatgatc taccgaaaga ttaccaagag ccggatcctt atctggatga gacggtgaat
    10621 atcgcactcg atctggcgaa gcttgaaaaa gccagacccg cggaacaacc cgctcccgtc
    10681 aagtaatatc aatcaggcac aagaaattgt gcctgatttt ttaacagcgg caagatgccg
    10741 taaatcagat gctacaaaat gtaaagttgt gtctttctgg tgacttacgc actatccaga
    10801 cttgaaaata gtcgcgtaac ccatacgatg tgggtatcgc atattgcgtt ttgttaaact
    10861 gaggtaaaaa gaaaattatg atgcgaatcg cgctcttcct gctaacgaac ctggccgtaa
    10921 tggtcgtttt cgggctggta ctgagcctga cagggataca gtcgagcagc gttcaggggc
    10981 tgatgatcat ggccttgctg ttcggttttg gtggttcctt cgtttcgctt ctgatgtcca
    11041 aatggatggc attacgatct gttggcgggg aagtgatcga gcaaccgcgt aacgaaaggg
    11101 aacgttggct ggtcaatact gtagcaaccc aggctcgtca ggcagggatc gctatgccgc
    11161 aagtggctat ctaccatgcg ccggacatca acgcttttgc aaccggtgcg cgccgtgatg
    11221 cctctctggt tgctgtcagc accggtttgc tgcagaacat gagcccggat gaagccgagg
    11281 cggtaattgc tcacgaaatc agccacatcg ccaatggtga tatggtcacc atgacgctga
    11341 ttcagggcgt ggtgaacacc ttcgttatct ttatttcccg tattctggcg cagcttgccg
    11401 cgggttttat gggcggaaat cgtgatgaag gtgaagagag caacggcaac ccgctgatct
    11461 actttgcggt tgcaacggtt ctggaactgg tgtttggtat tctggcgagc attatcacca
    11521 tgtggttctc gcgtcatcgt gaattccatg ctgatgccgg ttcggcaaaa ctggttggtc
    11581 gcgagaaaat gattgccgcg ctgcagcgcc tgaaaaccag ctatgaaccg caagaagcaa
    11641 ccagcatgat ggctttctgc attaatggta agtcgaaatc gctcagtgag ttgttcatga
    11701 ctcacccgcc gctggataaa cgaattgaag ctctgcgtac gggtgaatac ctgaagtaat
    11761 atgaagtaaa tactaaaaag tgcgtcctga tcg
//
LOCUS       DAJTMC010000129        11575 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.129, whole genome shotgun sequence.
ACCESSION   DAJTMC010000129 DAJTMC010000000
VERSION     DAJTMC010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11575
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.129"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(<1..985)
                     /locus_tag="OO130_003099"
     CDS             complement(<1..985)
                     /locus_tag="OO130_003099"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310582.1"
                     /note="PtrB; oligopeptidase that cleaves peptide bonds
                     following arginine and lysine residues; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HCR5366413.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRLRDEQQWEELIPPRENIMLEGFTLFTDWL"
     gene            complement(1194..1853)
                     /locus_tag="OO130_003100"
     CDS             complement(1194..1853)
                     /locus_tag="OO130_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HCR5366414.1"
                     /translation="MANWLNQLQSLLGQSSSSISSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVRDGIERDLEQQKRTLA
                     E"
     gene            complement(2180..2536)
                     /locus_tag="OO130_003101"
     CDS             complement(2180..2536)
                     /locus_tag="OO130_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HCR5366415.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(2603..2897)
                     /gene="yebG"
                     /locus_tag="OO130_003102"
                     /pseudo
     CDS             complement(2603..2897)
                     /gene="yebG"
                     /locus_tag="OO130_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
     gene            3031..4209
                     /gene="purT"
                     /locus_tag="OO130_003103"
     CDS             3031..4209
                     /gene="purT"
                     /locus_tag="OO130_003103"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HCR5366416.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSESLFREAVADIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWKYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCSDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVALATAESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(4265..4906)
                     /gene="kdgA"
                     /locus_tag="OO130_003104"
                     /gene_synonym="eda"
     CDS             complement(4265..4906)
                     /gene="kdgA"
                     /locus_tag="OO130_003104"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HCR5366417.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(4943..6754)
                     /gene="edd"
                     /locus_tag="OO130_003105"
     CDS             complement(4943..6754)
                     /gene="edd"
                     /locus_tag="OO130_003105"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HCR5366418.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQSPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRRFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(6989..8464)
                     /gene="zwf"
                     /locus_tag="OO130_003106"
     CDS             complement(6989..8464)
                     /gene="zwf"
                     /locus_tag="OO130_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HCR5366419.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            8801..9670
                     /gene="hexR"
                     /locus_tag="OO130_003107"
                     /gene_synonym="yebK"
     CDS             8801..9670
                     /gene="hexR"
                     /locus_tag="OO130_003107"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HCR5366420.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAAMALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            9798..11240
                     /gene="pyk"
                     /locus_tag="OO130_003108"
     CDS             9798..11240
                     /gene="pyk"
                     /locus_tag="OO130_003108"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HCR5366421.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(11300..11518)
                     /locus_tag="OO130_003109"
     CDS             complement(11300..11518)
                     /locus_tag="OO130_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001499009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5366422.1"
                     /translation="MEYQADYYITINDSIKTWVQTQYSKDSGLPMAVIGHSVAEEAGM
                     RRLASYLDLHSGYPCIHFTGGCDYDWIE"
BASE COUNT         2800 a         2981 c         2895 g         2899 t
ORIGIN      
        1 ccagccagtc ggtaaacagc gtaaaccctt ccagcatgat gttttcgcgt ggcggaatta
       61 actcttccca ctgttgctca tcacgcaggc gggtacggta taagccaaag tttttgccgt
      121 ggcggttgga acgcagataa aaccgatgct ggtagtgatc aaggctgtat tcgtgatctt
      181 tgcggcgcgg cagaaaaaca aacggctcgg catcggccat ttccgcgtcc agcaggcgaa
      241 cttcactggt ggtggcgctg gccaaatgaa tgactacata gtgcttcgag gtcgttttat
      301 gcaggctgac gtaataggta tcgtcttttt cttcgtagat cagtttatct tgcgatgctg
      361 gcgtaccgat ggcgtgacgc cagacctgat aaggcagcag cgtcaccgga tgcttgcgaa
      421 catagtagaa agtccaggag tcatttgccc agacaaagct gggttcaacg ttatccagca
      481 gttccgggta ccagttacca gtttccagat tacgaaaacg aatgccgtac tggcgtcggg
      541 aaagaaaatc ttctgccagc gccataatgg tgttatcggg cgtaatcgcc attccgccca
      601 tcgaataaaa ctcactatga gctgcgcgct tattggcatc gagcaatgtt tcccactcat
      661 cccactcttc actgaatgcc gattgacgct ggtagatagc atattcacag cctggttcat
      721 aaatatgccg atagcggtag ccatttttga tgtagggcgc agaaacttct cgttgcggaa
      781 tgcggtcgat gatttccttt aagatgcgat cctgcaaggc ttgttgtgag gccatcaccc
      841 gatgaccgta actattttct tgttgcaggt agtccaggac ttctggctga gaacgcgtat
      901 cgtcccgcag ccagtagtaa ttatcgatgc gcgtatcgcc atgaagcgtc atggcgtggg
      961 gaatgcgggc ggcttttggt agcatgttat tgttctttct ggttgaaaca tcttataagg
     1021 gtggcaaaac tcaccgggga tgcaagcgaa acaggggagt cattgcttag atgatgacag
     1081 gtaatggcgc ggatatcgaa tgttatgcag acagagaaaa tcagcctgtt caaatggctg
     1141 tgcgattctg gatagcccga aatagtcaac ttcaggctat ccagagagcg gaattattcc
     1201 gccaaagtgc gtttttgctg ttcgagatcg cgttcaatgc cgtcacgaac atcctggggg
     1261 attttcagcg cgtcacccag tgcattcagg taactgcgtt ccataaaatg gtcaatatca
     1321 atagccgcgc aactcaggaa atagatttcc agcgcctctt cttcattgcg gaccccggta
     1381 gccaagcgtt gtggatccag cggttgttcg attgcctgct caatgagtac acgcccctgc
     1441 tcttccacgc cggcttcacg caattgctgg tcgattgccg cacgttcttt ggcatcaata
     1501 tgaccatcac ttttagcggc aaagaccagc gcaaggatca aacgttctgt acgctcatcc
     1561 agcggcgtac tttgcgcgcc aaactgcggt tcgtcctgat gcgccgcgcg aattttatct
     1621 ttgtatttat tccacagcac cgtacccgct accgctccgc cgccaaccag taacgcgttg
     1681 gtgccatatt tcgtaagaag tttacgtgct gatttatttg cgaccagcag cccagccagt
     1741 ccgcctaatg cgcctggcac taacagtttg cccaatccct gatccgcaga cgaggagata
     1801 gaagaactgc tttgcccaag cagggattgc agttgattta accagttagc catatttgct
     1861 cctcaataac catttttagt gcctgtagca tagcggatga ggatgtcaga aaatgtatat
     1921 cgagacaaaa gatgcgcaaa taagcggggc ggtaggccgt tattcaaaga aattcgcgcc
     1981 aggtaaggta tttactgtat atcaggtgat aagggtattt taggtgaaag tagtttgtcg
     2041 ttttgagtgg cattgtttga tgctgattgc ccgatgcgac gctgttgcgt cttctcaggc
     2101 ctacgattgt ggtgcgttct ggggcggaca aggcactcgc gccgcatccg ccaacggtca
     2161 atcgaattat cagaagccat taacgccgct gatattccgc cattcccgct ttgcagtcca
     2221 cgctgacctg gtaatgaata tcggcacttt taccacgcac ggttagcggt actgaccatt
     2281 tatcatcttt accctgaatg tcctgcaaac tgacccaagc cacgggatcg gcctgaccga
     2341 caattttttg atcatctgcc caacgcgcca cgcgattttg ttgataatca cgttttacgc
     2401 tcgcggcaat tccggcagca tccagacctt cacactttgg gaaagtgacc gacttgctgg
     2461 tttcattatt ggcagcgaaa actgatgcgc aggcagaaac caacaacagc cctaaaaacg
     2521 cccctctttt tttcatgttt ttctccatag cacaatgatt caggagaaag catggtacaa
     2581 attgtcagga gcgcaagttg cttcaggccg cgtgtgaggc atcttcctct tcggattcag
     2641 caccgacgac ctgtgggtgg ggacggtaat ttgccggttt tcagaatggt gctcagcaca
     2701 tctttttgtt ctgccagcca tagcgaaagg gcttcacgtt gctcgtcttc catttgcact
     2761 ggagaattag tcagccaggt gtcaagcaga tccgccgtat caagcatttt gtcataagca
     2821 tcggcttcct ttttgctggt aaacgacatt ttctcttcgc cctcacgaat gactacgtat
     2881 ttaacttcaa ccgccatttg cagcctctca taataactgt gattttatac agtgtatttc
     2941 ttttcggttg agaaatcaaa atcaacaata aagacacacg caaacgtttt cgtttatact
     3001 gcgcgcggaa ttaatcaggg gatattcgtt atgacgttat taggcactgc gctgcgtccg
     3061 gcagcaactc gcgtgatgtt attaggctcc ggtgaactgg gtaaagaagt ggcaatcgag
     3121 tgtcagcgtc tcggcgtaga ggtgattgcc gtcgatcgct atgccgacgc accagccatg
     3181 catgtcgcgc atcgctccca tgtcattaat atgcttgatg gtgatgcatt acgccgtgtg
     3241 gttgaactgg aaaaaccaca ttatatcgtg ccggagatcg aagctattgc caccgatatg
     3301 ctgatccaac ttgaagagga aggactgaat gttgtcccct gcgctcgcgc aacgaaatta
     3361 acgatgaatc gcgagggtat ccgtcgcctg gcggcagaag agctgcagct gcccacttcc
     3421 acttatcgtt ttgccgatag cgaaagcctt ttccgcgagg cggttgctga cattggctat
     3481 ccctgcattg taaaaccggt gatgagctct tccggcaagg ggcagacgtt tattcgttct
     3541 gcagagcaac ttgctcaggc atggaagtac gctcagcaag gcggtcgcgc cggagcgggc
     3601 cgcgtaattg ttgaaggcgt cgttaagttt gacttcgaaa ttaccttgct aaccgtcagc
     3661 gcggtggatg gcgtccattt ctgtgcaccg gtaggtcatc gccaggaaga tggcgactac
     3721 cgtgaatcct ggcaaccaca gcaaatgagc ccgcttgccc ttgaacgtgc gcaggagatt
     3781 gcccgtaaag tggtgctggc actgggcggt tatggtctgt ttggtgtcga gctgtttgtc
     3841 tgtagtgatg aggtgatttt cagtgaggtc tcccctcgtc cacatgatac cgggatggtg
     3901 acgttaattt ctcaagatct ttcagagttt gccctgcatg tacgtgcctt cctcggactt
     3961 ccggttggcg ggatccgtca gtatggtcct gcagcttctg ccgttattct gccacaactg
     4021 accagtcaga atgtcacgtt tgataatgtg cagaatgccg taggcgcaga tttgcagatt
     4081 cgtttatttg gtaagccgga aattgatggc agccgtcgtc tgggtgttgc gctggcgacc
     4141 gctgagagtg ttgttgacgc cattgaacgc gcgaagcacg ccgccggaca ggtaaaagta
     4201 cagggttaaa cccggcaaaa aaacgctaca aaaatgcccg atcgtagatc gggcgttttg
     4261 acttttacag cttagcgcct tctacagctt cacgcgccag cttagtaatg cggtcgtaat
     4321 cgcccgcttc cagcgcatct gccggaacca gccaggaacc accgatgcac agcacgcttt
     4381 tcagcgccag gtagtcacgg tagttagccg gagaaatacc acccgtcggg cagaaacgga
     4441 cctgggagaa cggacccgcg atcgcctgca gggctttcac gccgccgtta gcttcagccg
     4501 ggaagaattt gaactctttc aaaccgtagt ccatacccag catcagttcg gaaacagtgc
     4561 tgatccccgg aattagagga atagtccctt cggtagcagc tttcagcagc ggctcggtca
     4621 gacccgggct aattgcgaac tgtgcacccg cttcagtgac ttctgccagc tgctgtggat
     4681 tcagcaccgt accggcaccc acaatcgctt caggcacttc tttggcgata gcacggatag
     4741 cgtcaactgc acactcggta cgcagagtca cttccagaac gcgcacccca ccagcaacca
     4801 acgcttttgc catcggcact gcgtgttcca gtttttttac cacgataacc ggtacaaccg
     4861 ggccggtggt caggattgat tctgcacttg ttttccagtt tttcatcaga gttttctctc
     4921 gcctgattac aaatttgtcg tcttaaaaag tgatacaggt tgcgccctgt tcggcaccgg
     4981 acagtttttc acgcaaggcg ctgaataatt cacgtcctgt ccccacgcgt gacgcgctca
     5041 ggtcaggaat gtgcggttcg cgagcagcca gttccgcttc gtctaccagc agcgtcagtt
     5101 cgcctgtctg tccattcaca cgaatgatgt ccccgtcgcg cacttttgcc agcagcccgc
     5161 catcgtaggc ttctggtgtt acgtggatag ctgacggcac tttacctgaa gcgccggaga
     5221 gtcgtccatc ggtaactaac gcaattttga aacgccggtc caataataca ccaagtggcg
     5281 gcatgagttt atgtaattct ggcattccgt tcgcttttgg cccctgatga cggacaacaa
     5341 cgacacaatc gcggtccagc aaacccgctt caaaggccgg cataacgtca tgctggcttt
     5401 caaaaacaac cgctggcgct tcaatcacct ggttctcaac cggcacggca gaggttttca
     5461 taaccgcacg gcccaggtta ccgcttaaca cttttgtccc accatgatga gagaaaggtt
     5521 gttcgaagga agcgatcaca ttgctgtcga gtgatttttc cgccccttcc cgccagtcca
     5581 gttcaccatt attcagccat ggttcaaggg tataacgaga cagaccaaag cctgccaccg
     5641 tattgacatc ttcatgcagc aggcctgctt tgagcagttc acgcaccaga accggtacgc
     5701 cacctgccgc ctggaagtgg ttaatatcgg ccggaccgtt cgggtagaga cgtgccatca
     5761 gcggtacaac atcagaaagg tcagagaagt catcccagtt aatctgaata ccggccgcgc
     5821 gcgccatcgc caccaggtgc atggtgtggt tagtggaacc accggtcgcc agcagtgcaa
     5881 cgataccgtt caccaccact ttctcatcga tcatcttacc gatcggcatc cattcattac
     5941 cattaccggt catgcgtgta acctgacgcg cagcggcagc ggtcaaagca tcgcgcagcg
     6001 gagaatccgg atgaacaaaa gaagagcctg gcgactgcat ccccataaat tccaccacca
     6061 tctggttggt gttggcagta ccgtagaaag tacatgttcc cggcgcatgg taagacgcgg
     6121 cttctgactc cagtaaggcc atgcggtcca ctttaccttc ggcataaagc tggcgaatac
     6181 gcactttttc tttatttggc aaaccgcttg ccatcggtcc agacggcaca aacaccgcag
     6241 gcaaatgacc aaacgacagg gctgccatcg tcagacccgg gacaatcttg tcgcacacac
     6301 cgaggaacag agcaccatca aacatgttat gggacagccc caccgccgca gacatcgcta
     6361 tcacttcgcg gcttagcagc gacaattcca ttccatcctg cccctgggtg acaccatcac
     6421 acatcgccgg aacaccgccc gcaacctgac caaccgcatt cgcttcatgc agggctttac
     6481 gaatgatttc tggatagtgt tcataaggct ggtgcgcgga gagcatgtcg ttataggagg
     6541 tgatgatggc gatattgtta cgcaacatgc ttttcaaaga ggctttgtct tctggctggc
     6601 aggcagcgaa accgtgtgcc aggttaccgc atgccaactg cgaacgatga acggtcgacg
     6661 ttttcgcttg ttctatccgg gcgagataag cagagcgagt ctcgcgcgaa cgttcaatga
     6721 ttcgatttgt tacgcgtaac aattgtggat tcataaaggc tcctgaaatt gagttgtcag
     6781 agcaggatga ttcacaacgc gtttcattca gaggatttat gagtgaaacg cctgtaaccg
     6841 gagctcatag ggcaaaaacg tttcagtcag tgtaataaaa aaagcctcgt gggtgaatcc
     6901 gcacgaggcc tgaaagtgta aaaattgttc tacaatctgt gcaagatcat gttaccggta
     6961 aaataaccat aaaggataag cgcagatatt actcaaactc attccaggaa cgaccatcac
     7021 gggtaatcat cgccaccgag gcaacgggtc cccaggttcc ggcctgatac ggtttcggcg
     7081 catcattgtc catcgcccac gcctcagtaa tggagtctac ccatttccag gcttcttcca
     7141 cttcatcacg acgtacaaac agtgcctgaa taccacgcat ggtttccagc agcagacgtt
     7201 cataggcatc cgccagatgc gtctgattaa aggtttctga atagctcaga tccagcttgg
     7261 tgatttgcag gttatgtttg tggtcaaggc caggaacttt attcagtacc tggatatcca
     7321 cgccttcatc aggttgcaga cggatagtca gtttattctg cggcagatcc tgccacgact
     7381 ctttaaacag attcagttca ggtgttttga aatagaccac gacttcagaa catttggtcg
     7441 gcagacgttt accagtacgc aggtagaatg gcacaccggc ccagcgccag ttatcaatgt
     7501 cgacgcggat cgccacgaag gtttctgtat tgctgctctt gttcgcaccc tcttcttcca
     7561 gatatcccgg cacttttttg ccctgggcga agcccgcagt atattgccca cgtacggttt
     7621 tttcgcgtac gttggagcgg tcgatgcggc gcagggactt cagtactttc actttttcat
     7681 cgcggatgct gtctgcgctc aggtcagatg gcggagacat cgcaatcatg cagagaattt
     7741 gtagcaggtg gttctgaatc atgtcacgca tctgaccggc tttatcaaaa tagccccagc
     7801 gcccttcgat ccccacttct tctgccacgg taatctcaac atgatcaatg gtgcgattgt
     7861 cccagttatt cacaaacagg gagttagcaa aacgcagcgc caacaggttc agcaccgttt
     7921 ctttaccaag atagtggtcg atacggtaaa cctggcactc ctcgaagtat tcgccaacct
     7981 gatcattgat ttcctgcgag gtcgccagcg acgtccccag cggtttctcc atgactacgc
     8041 gtgccggttt agcattcagt tttgcctcgc caagcccttt gcaaattgcg ccaaaagtgc
     8101 tgggcggcat ggcaaagtag ttaatggtga tacgattttt ttgatccagc atcgcgccga
     8161 gacggttgaa tgcagcagtg tcattgacat cgagattaca aaaatccaga cgtgcactca
     8221 gggtgtccca taaaccttca tcaatggttt ctttcatgaa agtttcgagc gcctcgcgga
     8281 caactttggt atatgccgct ttatcccagt cagcacgccc tacgccgata atccgggtgt
     8341 ccgggttgag ctgaccggct ttttccagtt gatacaggga aggcagcaat ttacgacgcg
     8401 caaggtcgcc tttcgcgccg aaaatgacca ggtcacaggc ctgggctgtt tgcgttaccg
     8461 ccatgtcatt ctccttaagt taactaaccc ggtacttaag ccagggtata cttgtaattt
     8521 tcttacggtg cactgtactg cttttacgag cttgcgaaaa ctgtaaacgc ttatccaccc
     8581 gtgcgattac gggaaaagcg cgcaaagcgc ggcaaaactg ataaaaaatc atcgttttcc
     8641 attcggtaaa aatctgacac tgatcatgtt atgaaaaaaa ataacaactt ttttatctgc
     8701 ttttgtcatt aacacggcac acaggcgtaa tatctgacaa aactgcattt cgatttcttt
     8761 cagtgcggaa atcgtcatta cccgtgagtc tctttacatc atgaatatgc tggaaaaaat
     8821 ccagtctcag ctggaacatt tgagcaaatc agagcgcaaa gttgccgagg tcattctggc
     8881 ttcgcccgat aacgcgatcc attcgagtat tgctgctatg gcactggaag ccaatgttag
     8941 cgaaccgacg gtgaatcgtt tctgtcgcag catggacacg cgcggttttc ctgattttaa
     9001 acttcatctg gcacagagtc tggcgaatgg cactccctat gttaatcgca atgtcaatga
     9061 agatgacagc gttgaatcat acacagggaa aatatttgag tccgcaatgg caacgcttga
     9121 tcatgtccgt cattcactgg ataaatctgc catcaaccgc gccgtcgact tgctcactca
     9181 ggcaaaaaaa atcgcctttt tcggattagg ctcttcagcc gccgttgccc acgatgcgat
     9241 gaataagttc tttcgtttta atgttccggt ggtgtactcc gatgatatcg tgctgcaacg
     9301 catgagttgt atgaattgta gcgacggaga cgtggtggtg ctgatttctc acactggaag
     9361 aacaaaaaat ctggtcgagc tggcgcagct ggctcgcgaa aacgacgcaa tggtgattgc
     9421 cctcacctct gcgggtaccc cgctcgcccg ggaagcaacg ctggcaatta ccctcgacgt
     9481 accggaagat actgacattt atatgcccat ggtttctcga cttgcacagc tgaccgtgat
     9541 agatgtgctg gcgacaggat ttactttgcg acgcggtgca aaattcagag ataacttgaa
     9601 gcgggtcaag gaagcgctga aggaatcgcg ttttgataag cagttactta atttaagtga
     9661 cgatcgctaa aaacgactgt cactgtccta atcttatacg acatccgaat gagattaatt
     9721 tatcgccatc gcggcgttat ttcattcgga tttcatgttc aagcaacacc tggttgtttc
     9781 agtcaacgga gtattacatg tccagaaggc ttcgcagaac aaaaatcgtt accacgttag
     9841 gcccagcaac agatcgcgat aataatcttg aaaaagttat cgcggcgggt gccaacgttg
     9901 tacgtatgaa cttttctcac ggctcgcctg aagatcacaa aatgcgcgcg gataaagttc
     9961 gtgagattgc cgcaaaactg gggcgtcatg tggctattct gggtgacctc caggggccca
    10021 aaatccgtgt atccaccttt aaagaaggca aagttttcct caatattggg gataaattcc
    10081 tgctcgacgc caacctgggt aaaggtgaag gcgacaaaga aaaagtcggt atcgactaca
    10141 aaggcctgcc tgctgacgtc gtgcctggtg acatcctgct gctggacgat ggtcgcgtcc
    10201 agttaaaagt actggaagtt cagggcatga aagtgttcac cgaagtcacc gtcggtggtc
    10261 ccctctccaa caataaaggt atcaacaaac ttggcggcgg tttgtcggct gaagcgctga
    10321 ccgaaaaaga caaagcagac attaagactg cggcgttgat tggcgtagat tacctggctg
    10381 tctccttccc acgctgtggc gaagatctga actatgcccg tcgcttggca cgcgatgcag
    10441 gatgtgatgc gaaaattgtt gccaaggttg aacgtgcgga agccgtttgc agccaggatg
    10501 caatggatga catcatcctc gcctctgacg tggtaatggt tgcacgtggc gacctcggtg
    10561 tggaaattgg cgacccggaa ctggtcggca ttcagaaagc gttgatccgt cgtgcgcgtc
    10621 agctaaaccg agcggtaatc acggcgaccc agatgatgga gtcaatgatt actaacccga
    10681 tgccgacgcg tgcagaagtc atggacgtag caaacgccgt tctggatggt actgacgctg
    10741 tgatgctgtc tgcagaaact gccgcagggc agtatccgtc agaaactgtt gcagccatgg
    10801 cgcgcgtttg cctgggtgcg gaaaaaatcc cgagcatcaa cgtttctaaa caccgtctgg
    10861 acgttcagtt cgacaatgtg gaagaagcta ttgccatgtc agcaatgtac gcagctaacc
    10921 acctgaaagg cgttacggcg attattacca tgaccgaatc gggtcgtacc gcgctgatga
    10981 cctcccgtat cagctctggt ctaccaattt tcgccatgtc gcgccatgaa cgtacgctga
    11041 acctgactgc tctctatcgt ggcgttacgc cggtgcactt tgatagcgct aatgacggcg
    11101 tagcagctgc cagcgaagcg gttaatctgc tgcgcgataa aggttacttg atgtctggtg
    11161 acctggtgat tgtcacccag ggcgacgtga tgagtaccgt gggttctact aataccacgc
    11221 gtattttaac ggtagagtaa tctcacctgc cccggccgct gatggccggg gtatttagca
    11281 acacataaat taggactact tattcaatcc aatcataatc acagccccct gtaaaatgaa
    11341 tacagggata gcctgaatgg agatctaaat agcttgcaag acgtcgcata cccgcttcct
    11401 ctgcaacact atgtccaata actgccatcg gtaagccact atctttggag tattgtgtct
    11461 gcacccatgt tttaattgaa tcgttaatcg taatataata atcagcctga tattccatat
    11521 attgtattgg atcacaaaaa cagcctgccc caatgccaac agttctgact actct
//
LOCUS       DAJTMC010000130        11485 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.130, whole genome shotgun sequence.
ACCESSION   DAJTMC010000130 DAJTMC010000000
VERSION     DAJTMC010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11485
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.130"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            <1..154
                     /locus_tag="OO130_003110"
     CDS             <1..154
                     /locus_tag="OO130_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5366423.1"
                     /translation="LQLCRNDSLMIGRESFSVDRITPDDGGCSYIRLRREGAGNVRAG
                     GYYEGA"
     gene            141..728
                     /locus_tag="OO130_003111"
     CDS             141..728
                     /locus_tag="OO130_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001214489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5366424.1"
                     /translation="MRGLENAIRNLNSLDTRMVPQASAWAVNRVAAKVVSVATRQVAR
                     NTVAGDNQVKGGPLKLVRQRVRVFKASPAGKMYARIRVNRGNLPAIKLGTTCRRAGDG
                     LKVGKYFFRGAFIQQLANGRWHVLRRLPEACFETGKDSKGRPRKNRLPVEVVKIPLAG
                     PLTQAFEDARDRIIAAEMPKQLGYALKQQLRIHLG"
     gene            725..1126
                     /locus_tag="OO130_003112"
     CDS             725..1126
                     /locus_tag="OO130_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5366425.1"
                     /translation="MNRHTQIRQAVLASLKGECGGNVVLFDGLPAFIDAQELPAVAVW
                     LSDAQYTGKMTDEDDWQAVLHIAVFVRAQAPDSELDTWMENTIFPAMKDIPVLSGLID
                     TMIPLGFNYQRDNEMATWAMAEITYQITYTN"
     gene            1137..1877
                     /locus_tag="OO130_003113"
     CDS             1137..1877
                     /locus_tag="OO130_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HCR5366426.1"
                     /translation="MTTPNPLAKTKGAGTTFWMYTGKGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTLATLAWRPGDSGQKKLVQLFDS
                     GEVCAFRIKYPNGTVDVFRGWLSSLGKTITSKEVMTRTVKISGVGRPYLAEEGAETVG
                     VTGLTVAPASVSVKAGATTTLTFTVKPDGASDKAISVHSSDPQTASVTLSGLVATVKG
                     VKQGSVSIVGMTSDGEFVAVAAVTVSAP"
     gene            1936..2322
                     /locus_tag="OO130_003114"
                     /pseudo
     CDS             1936..2322
                     /locus_tag="OO130_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707739.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
     gene            2331..2659
                     /locus_tag="OO130_003115"
                     /pseudo
     CDS             2331..2659
                     /locus_tag="OO130_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308863.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
     gene            2631..5672
                     /locus_tag="OO130_003116"
     CDS             2631..5672
                     /locus_tag="OO130_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR5366427.1"
                     /translation="MDQIANLVIDLGIDAAEFKNEIPRIKNLLNGAAGDAERSSARMQ
                     RFMERQTQAARQTMHAASSAATAASAHAQTVEKNARAHERMAREVEQTRLRVDALNQK
                     MREEQAQARALAEAQDKAAAAFYRQIDSVKQAGAGLQELQRIQQQIRQARNSGGVGQQ
                     DYLALISEITAKTRALTQAEEQATRQKAAFIRQLKEQATRQNLSSSELLRARAAQLGV
                     SSAAEVYIRKMERAGKATHSLGLKSAAARRELGVLISQMARGNFGALRGSGITLANRA
                     GWTGALMSPKGMMTGGVIGGLVAAVLGLGKAWHDGRKEGEEFNRQLALTGHYAGVTVG
                     QLWKLSRAISGNGITQHAAAGALAQVVGSGAFHGNDIGMVAKAAAQMERSVGQSVSDT
                     INQFKRLKDDPVNAAKALDNALHFLTATQLEQIRVLGEQGRSSDAARIAMSALAEETG
                     KRTSDIDNNLNALGSTLQTLSDWWKQFWDAAMNIGREDSLDAQIDALQEKIQRAKKYP
                     WTNASTQVEYDQQRLNDLQEKKRRKDLQDAKAQAERNYQEQQKRRNAENAALNRMNET
                     EAARHQREIARINAMQYADQAVRDAAIQRENERYEKAIKKNTRATRNDEATRLLLQYS
                     QQQAQVEGQIAAARQSAGIATERMTEAHKQLLALQQRISDLDGKKLTADEKSVLARKN
                     ELIQALTLLDVKQQELQKQTALNDLRKKTVQLTSQLADKERALREQHNLDIATAGMGD
                     KQRQRYQAQLRIRQEYRQQLQQLENDSRQKGTYGTEDYRRAEEVLKGSLKRQLNENKR
                     YWQELEVAQGDWKNGAMRAFQNFTADADNAVGTAEQMFTVAFSSAGNALATFCTTGKL
                     NFKSFTSSLLSDMARIMAQMAMMQAVKGVGSLFGFTTNADGGVYQSADLSRYSGTVVN
                     RPTFFAFAKGAGVMGEAGPEAILPLRRGADGKLGVVADTGGSGMVMFAPQYNIGINND
                     GTNGQIGPAALKVVYDLGKKAAADFMQQQARDGGRLSGAYR"
     gene            5672..6001
                     /locus_tag="OO130_003117"
     CDS             5672..6001
                     /locus_tag="OO130_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5366428.1"
                     /translation="METFRWKVRPDMNVVSEPKVVAVKLGDGYEQRRPAGLNNLLSTY
                     SVTIRVRKGEHPPLKAFLERHGGVRAFQWTPPYDWKPIRVACRKWSASVGALWVTVTA
                     DFEQVVN"
     gene            6001..6700
                     /locus_tag="OO130_003118"
                     /pseudo
     CDS             6001..6700
                     /locus_tag="OO130_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706643.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
     gene            6705..7448
                     /locus_tag="OO130_003119"
     CDS             6705..7448
                     /locus_tag="OO130_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5366429.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVRTPEGERYIPCVNISAEPE
                     AYFRIAPEDWLRAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRHGQNLYLDNLEAT
                     GLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHREWHASAFTGICNDLAAASTFV"
     gene            7385..7987
                     /locus_tag="OO130_003120"
     CDS             7385..7987
                     /locus_tag="OO130_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HCR5366430.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRALATQLPAFRQKLSD
                     GWYQVRIAGRDAGETELSARLNEPLANGAVIHIVPRLVGAKSGGVFQAVLGAAVMAVA
                     IWMPGVGIMASNLLFSLGASMTLGGVAQMLAPKARTPRTQTTDNGKQNTCFSSLDNMV
                     AQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            8048..>11485
                     /locus_tag="OO130_003121"
     CDS             8048..>11485
                     /locus_tag="OO130_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HCR5366431.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVDGPVDGLKS
                     VLLNGTPVLDSEGKTNFSGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRLTFSVQALVENTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVGNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGNQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMVWCR
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNVVEVNWIDPDNGHETATELVEDAQAILRYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLD
                     REITLPSSCTTLISLVDGNGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDRRGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVAADDGSERLVSTARTTETTYRFRQ
                     LALGNYSLTVRAVNARGQQGDPASVSFRIAAPAAPVTIELIPGYFQITAVPKLAVYDP
                     TVQFEFWFSEKRIADIRQVETSARYLGTALYWIAASINIRPGHDYYFYVRSVNTVGKS
                     AFVEAVGRASDDAEGYLSFYKGLINNTHLGKELWTQIDNGQLAPDLTEIRTSITNVSN
                     EITQTVNKKLENQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMKDGRLYIAGIGAGIEN
                     TPAGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNDVFLKRLTAPTITSGGNP
                     PAFSLTPGGRLTAKNADISGNVNANSGTLNNVTINKNCRVLGKLSANQIEGDLVKTVG
                     KPFPRDSRAPERWPSGTITVRVYDDQPFDRQIVIPAVAFRGAKHERKNNNIYSSCSLI
                     VKKNGAEIYNRTTLDNTLIYTGVIDMPAGHGHMTLEFSVSAWLVNGWYPTASISDLLV
                     "
BASE COUNT         2691 a         2719 c         3699 g         2376 t
ORIGIN      
        1 tttacagctg tgtcgcaatg attcgctgat gattggccgg gagtctttca gcgtggatcg
       61 tattactcct gatgacggcg gatgtagtta tatccggttg cggcgtgaag gagcaggaaa
      121 cgtaagggca gggggatatt atgagggggc ttgagaatgc catccgcaat ctgaacagtc
      181 ttgatacccg tatggtgccg caggccagcg catgggcagt aaaccgggtt gctgcaaaag
      241 ttgtctcggt tgccacccgg caggtcgcca ggaataccgt tgcgggagat aaccaggtga
      301 aggggggccc cctgaaactg gtgcgtcagc gcgtccgggt atttaaagcc agtccggcag
      361 gaaaaatgta tgcccgtatc cgtgttaacc ggggcaatct gcccgccatt aagcttggca
      421 caacatgccg gcgtgccggt gacggactga aggtggggaa atactttttt cgtggtgcgt
      481 ttatccagca gctggcgaac ggtcgctggc atgttctcag gcgtcttcct gaagcgtgtt
      541 ttgaaacagg aaaggacagt aaggggcggc ccagaaaaaa tcgtctgccc gtggaggttg
      601 tgaaaattcc gctggccgga ccgctgacac aggcatttga ggatgcccgc gaccgcatca
      661 ttgccgcgga aatgccgaaa cagctggggt atgccctgaa acagcaactg aggatacatt
      721 tgggatgaac aggcacacac aaatccgcca ggcagtactg gcaagcctga agggcgaatg
      781 cggggggaat gtcgtgcttt ttgacgggct tccggcattt attgatgcgc aggaactgcc
      841 cgccgtggcg gtgtggctga gtgatgcgca gtacaccgga aaaatgacgg atgaggacga
      901 ctggcaggct gttctgcata ttgccgtttt cgtccgtgca caggcgccgg attctgagct
      961 ggatacgtgg atggagaaca ccatatttcc ggccatgaag gatattccgg tgctttccgg
     1021 actcatcgac accatgatcc cactcggttt taactatcaa cgtgataatg agatggccac
     1081 ctgggcgatg gcggaaatca cgtaccagat cacgtacacg aattaaggag gtggcaatga
     1141 ccacaccaaa tccactggca aagacgaaag gtgcgggaac gacgttctgg atgtacaccg
     1201 gcaagggcga tgcgtttgcg aaccctttgt cggacactga ctggctgcgt cttgcgatgg
     1261 tgaaggatct gcaacctggc gaaatgaccg ctgatgcaga agatgacact tatctcgatg
     1321 atgaagatgc agactggaaa acgacaaccc aggggcagaa atccgtcggt gatactttgg
     1381 cgacgctggc ctggcgtccg ggtgacagcg ggcagaaaaa actggttcag ttgttcgatt
     1441 ccggtgaagt ctgcgcgttt cgtatcaaat accccaacgg tacggttgat gttttccgtg
     1501 gctggctgag ttcactgggt aaaaccatta cctcaaaaga ggtgatgacc cgtactgtaa
     1561 aaatcagcgg tgtggggcgt ccgtatctgg cagaggaggg cgctgaaacc gtgggtgtta
     1621 ccggactgac ggtggcaccg gcatctgtca gtgtcaaagc gggagcaacc accacactga
     1681 cctttacagt aaaacctgac ggggccagtg acaaagcgat cagtgtgcat tcgtcagatc
     1741 ctcagactgc ttcggtgacc ctgagcgggc ttgtggccac ggtgaaaggc gtgaagcagg
     1801 gcagtgtcag catcgtgggc atgacctctg acggagagtt tgtggcagtg gctgcggtga
     1861 ccgtcagcgc accataacag gacgatactc atcattgccc cggttatccg gggctttttg
     1921 catccggaga acatgatgtt tctgaaacag gatacgttta attatgaaaa acagtccgtg
     1981 gtgctcagtg agctgtccgg gctgcagaga attgaatatc tgacgtttgt tcagcagcga
     2041 acggcaaagt ttgatgcaca ggagggagaa ctgccggagg ctgaacgaca gattgctttt
     2101 ctgcgtatgg gaatggatat caatgcctgg ctggtttccc gctcactgtg gaatgctgag
     2161 cagtctcagg atgttgagac gctttgagca tccattatga caacatggtc gtatgatgcg
     2221 ctgggcgcgg gggcggagag ggttctgtcg ctgagcggta tggggaccat tgagaatgcc
     2281 ggggatgatg atcatgaggc gctgacgccg gaaaagtcct gacgcgggaa atgcagtttg
     2341 tcatgcggct tgcccgggag ttccggcggg cagactggcg gcggatgctg tcggaaatgt
     2401 cggccactga gcttggtgag ggggcgatta tttccggatg cagagcttca gtgatgtgtg
     2461 gatggatgcg cagtttgcct cgctgaaggc attgatcgtg agaatggtgt ccggcagcag
     2521 tgatgctgcg gtggctgatt tcagcctttt accggaagag aacgggatac cggagcgaac
     2581 ggacgaagaa ctgatgcatc ttggggaagg tatttccgga ggtgtgcgtt atggaccaga
     2641 tagccaacct ggtcattgat ttggggattg atgcggcaga gtttaaaaat gaaattcccc
     2701 gtatcaaaaa ccttctgaat ggtgcagccg gtgatgcaga acggtcttct gcccgtatgc
     2761 agcgttttat ggagcgtcag actcaggcgg cccggcagac aatgcatgcg gcttcttcgg
     2821 ctgcaacagc agcatcagcc catgcgcaga cggtggagaa gaatgcacgg gctcatgaac
     2881 gcatggcccg tgaggtggaa caaacccgtc tgcgcgtgga tgccctgaat cagaaaatgc
     2941 gcgaggaaca ggcgcaggcc agggcactgg cggaggcgca ggataaagcg gctgccgcct
     3001 tttatcgcca gattgacagt gtgaaacagg ccggtgcggg gcttcaggaa ttacagcgta
     3061 ttcagcagca gatccgacag gccagaaaca gtggcggggt tggtcagcag gattatctgg
     3121 cgctgatttc ggagatcacg gcgaaaaccc gtgccctgac gcaggcagag gaacaggcca
     3181 cccggcagaa agcggcgttt atccgccagc ttaaagagca ggcaacccgc cagaatctgt
     3241 cgtcttctga gttgcttcgc gccagggcgg ctcagctggg ggtaagcagt gctgcagaag
     3301 tgtatatccg caaaatggag cgggcaggaa aagccacaca ttcgctgggg ctgaaaagtg
     3361 cggcagcccg gcgggagctg ggggtgttaa tcagtcagat ggcgcgcggc aattttggtg
     3421 cgctgagggg atccgggata acgctggcta accgtgccgg atggacaggc gcactgatgt
     3481 cgccgaaagg catgatgact ggcggcgtta ttggcggact tgtcgcggcg gtcctgggtc
     3541 tgggtaaagc ctggcatgac ggccggaagg agggcgagga atttaaccgt cagctggcgc
     3601 tgacgggaca ttatgccggt gtcactgtcg ggcagttgtg gaaactcagc cgggccatat
     3661 ccgggaatgg tatcacgcaa catgcggcag ccggtgcgct ggcacaggtg gtgggcagtg
     3721 gggcatttca tggaaacgat attggtatgg tggcgaaagc tgccgcacag atggagcgat
     3781 cggttggcca gtcggtcagc gataccataa atcagtttaa gcggctgaag gatgatcctg
     3841 taaatgccgc gaaggcgctg gacaatgcgc tgcatttttt gactgccact cagcttgagc
     3901 agatacgcgt ccttggggag caggggcggt ccagtgatgc ggcccggatc gccatgtctg
     3961 cgctggcaga ggaaaccggt aaacgcacat ctgatattga taataatctc aatgcgctgg
     4021 gtagtacgct gcaaaccttg tctgactggt ggaagcagtt ctgggatgcg gccatgaata
     4081 ttggtcgtga agactcgctg gatgcgcaga ttgatgcgtt acaggaaaaa attcagcgcg
     4141 cgaaaaaata tccgtggaca aacgcctcca cacaggtgga gtacgatcag cagcgtctta
     4201 acgatcttca ggagaaaaaa cgccggaagg atttgcagga tgcaaaagcg caggcagaac
     4261 ggaattacca ggagcaacag aaacgccgga atgctgaaaa tgccgcgctg aaccggatga
     4321 atgaaacgga agctgcacga catcagcggg aaattgcgcg tattaatgcc atgcagtacg
     4381 ccgaccaggc tgtcagggat gcggcgatac agcgtgaaaa tgaacgttac gaaaaagcca
     4441 ttaagaaaaa tacacgggca acccgtaatg atgaggccac ccggttattg ctgcagtaca
     4501 gtcagcagca ggcacaggtg gaagggcaga ttgctgctgc cagacagtca gcaggcattg
     4561 ccactgaaag gatgactgaa gcgcataaac agcttctggc cctgcagcag cgcatcagcg
     4621 acctggacgg gaaaaagctg acggcagatg aaaagagtgt gctggcccgt aagaatgagc
     4681 tgattcaggc gctgacgctg ctggatgtga aacagcagga gctgcagaaa cagacagcgc
     4741 ttaacgacct gagaaaaaaa acggttcagc tgaccagcca gctggcagac aaagaacgtg
     4801 cactgcgtga gcagcacaat ctggatattg ccactgcagg tatgggggat aagcagcggc
     4861 agcgctacca ggcacagttg cgcatccggc aggaataccg gcaacagttg caacagcttg
     4921 agaatgacag tcgccagaaa ggcacttacg ggacggagga ctaccggagg gctgaggagg
     4981 tgctgaaggg gagcctgaag cgacaactga atgaaaacaa acgctactgg caggaactgg
     5041 aagtggcgca gggcgactgg aaaaacggtg ccatgcgggc gtttcagaat tttacggcgg
     5101 atgcggataa tgcggtggga acggcagaac agatgtttac agtggcattc agcagtgccg
     5161 gtaatgcact ggcgacattc tgtaccaccg gtaagctgaa ttttaaatcc ttcacctctt
     5221 ccctgttgtc agatatggcc agaattatgg cacagatggc catgatgcag gcggtaaagg
     5281 gcgtcggctc tttattcggc ttcacgacta atgctgatgg cggtgtttac cagtctgctg
     5341 atttgagtcg ctacagtggc acggtggtta accgtccgac gttttttgct tttgcaaaag
     5401 gcgcgggtgt gatgggggaa gcgggacctg aagccattct gccactgcgt cgcggtgctg
     5461 atggtaagct gggggttgtg gcggatactg gtggttcagg catggtgatg tttgccccgc
     5521 agtacaacat cgggatcaat aacgacggca cgaacgggca gataggtccg gcagcactga
     5581 aggtggttta tgacctcggg aaaaaagcgg ccgcggactt tatgcaacag caggcccgtg
     5641 atggtggccg gttaagtgga gcataccggt aatggagacg tttcgctgga aggtgcgccc
     5701 ggatatgaat gtggtatcag agccaaaagt ggtggcagtg aagctgggtg atggttatga
     5761 acagcgtcgc ccggcgggac tgaataatct gctgtcgact tacagcgtga cgatacgtgt
     5821 tcgtaaaggt gaacacccac ctttaaaagc ctttctggaa cggcacggtg gcgtccgcgc
     5881 atttcagtgg acgccacctt atgactggaa accgatcagg gtggcttgtc gtaaatggtc
     5941 ggcaagcgtg ggggcgttat gggtgactgt aacggccgat tttgaacagg tggtgaactg
     6001 atgcaggata tccggcagga aacactgaat gaatgcaccc gtgcggagca gtcggccagc
     6061 gtggtgctct gggaaatcga cctgacagag gtcggtggag aacgttattt tttctgtaat
     6121 gagcagaacg aaaaaggtga gccggtcacc tggcaggggc gacagtatca gccgtatccc
     6181 attcaggggg agtggttttg aactgaatgg caaaggcacc agtacgcgcc ccacgctgac
     6241 ggtttctaac ctgtacggta tggtcgccgg gatggcggaa gatctgcaga gtctggtcgg
     6301 cggaacggtg gtccggcgta aggtttacgc ccgttttctg gatgcggtga acttcgtcaa
     6361 cggaaacagt gacgccgatc cggagcagga ggtgatcagc cgctggcgca tcgagcagtg
     6421 cagcgaactg agcgcggtca gtgcctcttt tgtgctggcc acaccaacgg agacggatgg
     6481 cgcggttttc ccggggcgta tcatgctggc gaatacctgt atgtggacct accgttctga
     6541 tgagtgtggt tacacgggca gggcagtggc tgacgagttc gacaaaccaa cgacggatat
     6601 ccggaaggac aaatgcagca agtgtatgcg cgggtgtgag ttgcgcaaca ataccggtaa
     6661 tttcggcggt ttcctttcca tcaataaact ttctcagtaa atccatgaca cagacagaat
     6721 cagcgattct ggcgcacgcc cggcgatgtg cgccagcgga gtcgtgtggc ttcgtggtga
     6781 gaacgccgga gggggagcgg tatatccctt gtgtgaatat ttctgcagag ccggaggcgt
     6841 attttcgtat cgcaccggaa gactggctgc gggcagagat gcagggggag attgtggcac
     6901 tggtccacag tcatcccggt ggtctgccct ggctgagtga ggctgaccgg cggctgcagg
     6961 tgcagagtga tttgccgtgg tggctggtct gccggggggc gattcacaag ttccgctgtg
     7021 tgccgcatct caccgggcgg cgctttgagc acggggtgac ggactgttac acgctgttcc
     7081 gggatgctta tcatctggcg gggattgaga tgccggattt tcatcgcggg gatgactggt
     7141 ggcgtcacgg tcagaatctc tatctggata atctggaggc cacagggctg tatcaggtgc
     7201 cgttgtcatc agcacaaccg ggcgatgtgc tgctgtgctg ttttggttca tcggtgccga
     7261 atcatgccgc catttactgc ggcgacggtg agctgctgca ccatattcct gaacaactga
     7321 gtaaacgaga gaggtatacc gacaaatggc agcgacgcac acactccctc tggcgtcacc
     7381 gggaatggca cgcatctgcc tttacgggga tttgcaacga tttggccgcc gcatcgacct
     7441 tcgtgtgaaa acgggggctg aagccatccg cgcactggcc acacagctcc cggcgtttcg
     7501 tcagaaactg agtgacggct ggtatcaggt gcgcattgcc gggcgtgatg caggtgaaac
     7561 cgaattgtct gcccgtctta atgagccgct ggcaaatggt gcagtgatcc acatagtacc
     7621 gcgtctggtg ggagctaaaa gtggcggtgt gtttcaggcg gtgctggggg cagctgttat
     7681 ggcggttgct atatggatgc cgggggtagg aattatggcg agtaatctgc tgttttctct
     7741 cggtgccagt atgacgcttg gcggtgttgc acagatgctg gcaccgaaag ccagaactcc
     7801 ccgtacacag acaacggata acggcaaaca gaacacctgt ttctcctcac tggataacat
     7861 ggttgcccag ggcaatgttc tgcctgttct gtacggtgaa atgcgcgtgg ggtcgcgggt
     7921 ggtatctcag gagatcagca cggcagacga aggggatggt ggtcaggttg tggtgattgg
     7981 tcgctgatgc aaaatgtttc atgtgaaacc gcctgcgggc ggttttgtcg tttatggagc
     8041 gtgaggaatg ggtaaaggca gcagtaaggg gcataccccg cgcgaagcga aggacaacct
     8101 gaaatccacg cagttgctga gtgtgattga tgccatcagc gaagggccgg ttgacggtcc
     8161 ggtggatgga ttaaaaagcg tgctgctgaa cggtacgcca gtcctggaca gcgaggggaa
     8221 gacaaacttt tccggtgtta cggtggtgtt ccgcgccggc gagcaggagc agacaccgcc
     8281 ggaggggttt gaatcttccg gctcagagac tgtgctgggt acggaagtga aatacgacac
     8341 gccgatcacc cggaccatca cgtcggcaaa cattgatcgt ctgcgcctga ccttcagtgt
     8401 gcaggcactg gtggaaaaca cctcaaaggg ggaccggaat ccgtcggaag tccgcctgct
     8461 ggttcagata cagcgtaacg gtggctgggt gacggaaaaa gacatcacca ttaagggcaa
     8521 aaccacttcg cagtatctgg cctcggtggt ggtgggtaac ctgccgccgc gcccgtttaa
     8581 tatccggatg cgcaggatga caccggacag caccacagac cagctgcaga acaaaacgct
     8641 ctggtcgtcg tacaccgaaa tcatcgatgt gaaacagtgc tacccgaaca cggcgctggt
     8701 cggcgtgcag gtggattcag agcagttcgg taaccagcag gtgagtcgca attatcatct
     8761 tcgcgggcgc attctgcagg tgccgtcgaa ctataacccg cagacgcggc aatacagcgg
     8821 tatctgggac ggaacgctta agccagcata cagcaacaac atggtctggt gtcggtggga
     8881 catgctcact catccgcgct acggcatggg gaaacgtctt ggtgcggcag atgtggacaa
     8941 atgggcgctg tatgtcatcg gccagtactg cgaccagtca gtgccggacg gatttggcgg
     9001 cacggagccg cgcatcacct gtaacgctta cctgaccaca cagcgtaagg cgtgggatgt
     9061 tctcagtgat ttctgctcgg cgatgcgctg tatgccggta tggaacgggc agacgctgac
     9121 gttcgtgcag gaccgaccgt cggataaagt gtggacctat aaccgcagta atgtggtgat
     9181 gccggatgat ggcgcgccgt tccgctacag cttcagcgcc ctgaaggacc gccataatgt
     9241 cgttgaggtg aactggattg atccggacaa cggtcatgag acggcgacag agcttgtgga
     9301 ggacgcgcag gccattctcc gttacggtcg taacgtcacg aagatggatg cctttggctg
     9361 taccagccgg ggacaggcgc accgcgccgg gctgtggctg attaaaacgg aactgctgga
     9421 aacgcagacc gtggacttca gcgtcggcgc agaagggctt cgccatgtac cgggtgatgt
     9481 cattgaaatc tgcgatgatg attatgccgg tatcagcacc ggtggtcgcg tactggcggt
     9541 gaacagccag acccggacgc tgacgctcga ccgtgaaatc acgctgccgt cctcctgtac
     9601 cacgctgata agcctggttg acggaaatgg caatccggtc agcgtggagg ttcagtccgt
     9661 caccgacggc gtgaaggtga aagtgagccg ggttcctgac ggcgttgcag aatacagcgt
     9721 gtgggggctg aagctgccga cgctgcgcca gcgcctgttc cgctgcgtga gtatccgtga
     9781 gaacgacgac ggcacgtatg ccatcaccgc cgtgcagcat gtgccggaaa aagaggccat
     9841 cgtggataac ggggcacact ttgacggtga ccggcgcggc acggtgaatg gtgtcacgcc
     9901 gccagcggtg cagcatctga ccgccgaagt caccgcagac agcggggaat atcaggtgct
     9961 ggcgcgatgg gacacgccga aggtggtgaa gggtgtgagc ttcctgcttc gcctgaccgt
    10021 ggcagcggac gacggcagtg agcggctggt cagcacagcc aggacgacgg aaaccacata
    10081 ccgcttcagg cagctggcgc tggggaatta cagtctgaca gtccgggcgg taaatgcccg
    10141 ggggcagcag ggcgatccgg cgtcggtatc gttccggatt gcggcaccgg cagcgcctgt
    10201 cactattgaa ctgataccgg ggtattttca gataacggcg gtcccgaaac tggctgtata
    10261 cgacccgacg gtgcagtttg agttctggtt ttcggaaaag cggattgcgg atatcaggca
    10321 ggttgaaacc agcgcccgct atcttggcac ggcgctgtac tggatagccg ccagtatcaa
    10381 tatcaggccg ggccatgatt attattttta cgttcgcagt gtgaacaccg ttggcaaatc
    10441 ggcatttgtg gaggccgtcg gtcgggcgag cgatgatgcg gaaggttatc tgagttttta
    10501 taaagggttg atcaataaca cgcatctcgg caaggagttg tggacgcaga ttgataacgg
    10561 tcagcttgcg ccggacctga ctgaaatcag gacgtccatt acgaatgtca gcaatgaaat
    10621 cacgcaaacc gtcaataaaa aactggaaaa tcagagtgct gcaatccagc agatacagaa
    10681 agttcaggtt gatacaaata ataacctgaa cagcatgtgg gctgtgaagc tgcaacagat
    10741 gaaggacgga cgcctttata ttgcgggtat cggtgccggt attgagaata cgccagcagg
    10801 aatgcagagt caggtgctgc tggcggcaga caggattgcg atgattaatc ctgcgaatgg
    10861 caacacaaag ccgatgtttg ttggtcaggg cgatcagata ttcatgaacg acgtgttcct
    10921 gaaacgcctg acggctccga ccattaccag cggcggtaat cctccggcat tttccctgac
    10981 acctggcgga cggctgacgg cgaaaaatgc cgatatcagc ggtaacgtga acgcgaactc
    11041 cgggacgctc aacaacgtca cgattaataa gaactgtcgg gttctgggaa aattgtccgc
    11101 gaaccagatt gaaggcgatc tcgttaaaac agtgggcaaa cctttcccac gggactcccg
    11161 ggcaccggag aggtggccat cagggaccat taccgtcagg gtttatgacg atcagccgtt
    11221 tgatcggcaa attgttattc ccgcggtggc gtttcgcggt gctaaacatg agcggaagaa
    11281 taacaatatt tattcgtcat gcagcctgat agtgaagaaa aacggtgctg aaatttataa
    11341 ccgaacgacc ctggataata cgctgatata tacgggtgtt attgatatgc ctgccggtca
    11401 cggtcacatg acgctggagt tttctgtatc ggcatggctg gtaaatggct ggtatcccac
    11461 agcaagtatc agcgatttgc tggtt
//
LOCUS       DAJTMC010000131        11406 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.131, whole genome shotgun sequence.
ACCESSION   DAJTMC010000131 DAJTMC010000000
VERSION     DAJTMC010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11406
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.131"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(<1..748)
                     /gene="mak"
                     /locus_tag="OO130_003122"
     CDS             complement(<1..748)
                     /gene="mak"
                     /locus_tag="OO130_003122"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HCR5366432.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRAHISGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFAMDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIV"
     gene            873..1784
                     /gene="rdgC"
                     /locus_tag="OO130_003123"
     CDS             873..1784
                     /gene="rdgC"
                     /locus_tag="OO130_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HCR5366433.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(1862..1945)
                     /locus_tag="OO130_003124"
     CDS             complement(1862..1945)
                     /locus_tag="OO130_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5366434.1"
                     /translation="MTQRPWSKLQHKTHNIAALKIIVRRSE"
     gene            complement(1942..2283)
                     /locus_tag="OO130_003125"
                     /pseudo
     CDS             complement(1942..2283)
                     /locus_tag="OO130_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            complement(2431..2715)
                     /gene="ppnP"
                     /locus_tag="OO130_003126"
     CDS             complement(2431..2715)
                     /gene="ppnP"
                     /locus_tag="OO130_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HCR5366435.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(2787..3464)
                     /locus_tag="OO130_003127"
     CDS             complement(2787..3464)
                     /locus_tag="OO130_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HCR5366436.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLPVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            complement(3722..3913)
                     /gene="yaiA"
                     /locus_tag="OO130_003128"
     CDS             complement(3722..3913)
                     /gene="yaiA"
                     /locus_tag="OO130_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HCR5366437.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            complement(3963..4487)
                     /gene="aroL"
                     /locus_tag="OO130_003129"
     CDS             complement(3963..4487)
                     /gene="aroL"
                     /locus_tag="OO130_003129"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HCR5366438.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(4670..5128)
                     /locus_tag="OO130_003130"
     CDS             complement(4670..5128)
                     /locus_tag="OO130_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HCR5366439.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            5248..6057
                     /gene="proC"
                     /locus_tag="OO130_003131"
     CDS             5248..6057
                     /gene="proC"
                     /locus_tag="OO130_003131"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HCR5366440.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPNKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(6074..7189)
                     /gene="adrA"
                     /locus_tag="OO130_003132"
                     /gene_synonym="dgcC"
     CDS             complement(6074..7189)
                     /gene="adrA"
                     /locus_tag="OO130_003132"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HCR5366441.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(7291..7611)
                     /gene="psiF"
                     /locus_tag="OO130_003133"
     CDS             complement(7291..7611)
                     /gene="psiF"
                     /locus_tag="OO130_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HCR5366442.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(7730..9145)
                     /gene="phoA"
                     /locus_tag="OO130_003134"
     CDS             complement(7730..9145)
                     /gene="phoA"
                     /locus_tag="OO130_003134"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HCR5366443.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVSDAASLNAVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKDGNTLVIVTADH
                     AHASQIVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(9246..9506)
                     /gene="iraP"
                     /locus_tag="OO130_003135"
     CDS             complement(9246..9506)
                     /gene="iraP"
                     /locus_tag="OO130_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HCR5366444.1"
                     /translation="MKNLIAELLLKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIEQVEGALYEVKPDASIPDDDTELLRNYVKKLLKHPRQ"
     gene            complement(9733..9890)
                     /locus_tag="OO130_003136"
                     /pseudo
     CDS             complement(9733..9890)
                     /locus_tag="OO130_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            9968..11062
                     /gene="ddlA"
                     /locus_tag="OO130_003137"
     CDS             9968..11062
                     /gene="ddlA"
                     /locus_tag="OO130_003137"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCR5366445.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPSKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLRLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(11086..11298)
                     /locus_tag="OO130_003138"
     CDS             complement(11086..11298)
                     /locus_tag="OO130_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HCR5366446.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGAVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
BASE COUNT         2844 a         2955 c         2725 g         2882 t
ORIGIN      
        1 ggacaatcac atccggatcg agaatattca cgacatgtgc cagcgatttt gccagccgca
       61 gctcgtagcg acgcaatgcc agttccgcta ccggatcgct ttcttcaacc aggcggataa
      121 tttcactgcc tttcagcgca tgtccgctca aacgacgata atccatcgcg aatcccgtgc
      181 ccgaaataaa ggtttcaata caaccttgtt taccgcaata acaagggact tcctcgcgat
      241 aacgcagttc gtcttcgtcc atccacggta gcggattgtg tccccactca cctgccgtgc
      301 cattgccgct gatatgcgcc cgcccattga atgccacgcc cgcgccgcat cccgtgccga
      361 taatcacggc aaataccgtc tgcgctcccg ctgccgcgcc atctactgct tctgaaaccg
      421 ccagacagtt agcgtcattt gccagccgca cttcccgctg caacctcgcg cttaagtctt
      481 tatcgaatgg ctgaccgttg agccaggttg aattggcatt cttcaccaca ccggtgtaag
      541 gcgaaattga gccaggaatg cccataccta ccgttccgcg ctgccccgtc gcctgctccg
      601 ccatatcaac caacgtggcg atcgtttcaa tagtctgccg gtaatcatca cgcggcgtgg
      661 gcagacgatg gcggtacaac tgctcccctg catcgcccag tgcaatcact tcagttttgg
      721 tgccgcctaa atcgatacct atacgcacgg tactctcctt atttttttca atatcaatag
      781 cgtagagacg gacaaccgga ttggcaatgc aaggccgccg acaattcgtt atcatgcccg
      841 ctaaatttaa caacaaggcc gtggaaatta tcatgctgtg gttcaaaaat ttaatggttt
      901 accgtcttag ccgcgagatt tcgctgcgtg cagaagagat ggaaaaacag ctagcctcga
      961 tggcatttac cccatgcggc agccaggaca tggcgaagat gggctgggtt cctccgatgg
     1021 gatcgcacag cgatgcgtta acgcacgttg ccaatggtca aattgttatc tgcgcgcgca
     1081 aagaagaaaa aatcctcccg tctccggtga ttaagcaggc gctggaagcg aaaatcgcca
     1141 aactggaagc ggaacaggcg cgtaagctga agaaaaccga aaaagattcg ctgaaagacg
     1201 aagtgctgca ctctctgttg ccgcgtgctt tcagccgttt tagccagaca atgatgtgga
     1261 tcgacacggt taacggtttg attatggtgg actgcgccag tgccaaaaaa gcggaagata
     1321 cgctggcact gctgcgtaaa agcctggggt cgttacctgt ggtaccattg agcatggaaa
     1381 acccgattga actgacgctg accgaatggg ttcgctccgg tagcgcggca cagggcttcc
     1441 agctgcttga tgaagccgag ctgaaatcgt tgctggaaga tggcggcgtg atccgcgcga
     1501 agaaacaaga tctgaccagc gaagagatca ccaatcacat cgaagccgga aaagtggtga
     1561 ctaaactggc gctcgactgg cagcagcgca ttcagtttgt gatgtgcgac gatggttcgc
     1621 tcaagcgtct gaagttttgc gacgagctgc gcgatcaaaa cgaagatatc gaccgtgaag
     1681 atttcgccca gcgttttgat gccgatttca tcctgatgac tggtgaactg gcagcgttaa
     1741 ttcaaaacct gattgaagga ttaggtggcg aagcacaacg ttaattgctg attttccttt
     1801 aatgccggat gcgacgcctg ccgcgtctta tccggcgtac gaagccacac cagggatata
     1861 attattcgct acggcggaca ataattttta gcgcagcaat attatgcgtt ttatgctgta
     1921 acttgctcca tggacgttgt gtcattgttt ttcctcaagc cagtgacgcg cttcactgcg
     1981 aattttccgc attgccccgg ttttcactaa atcccgtaac agcgaaagat cgggttgttc
     2041 gacaaataag agtaatgatg gatcctcttt taaccacgtc gttttcacat cggcagcgag
     2101 ctgtggatta ttcatagcta gcgaccgatg ccgagactcg gtcaatgtcg ttaacaacga
     2161 cgccggggta tgtgcgttgc gtaatgccgt ttggcgcaaa tgatcattgc gactttgcag
     2221 caatgcacgc caccattcat catctgattt atcttttatc gtagccagtt ccgcaacaat
     2281 atctggagat cttcactgat atttatatca accttgatga attatgaata tatcggaaat
     2341 tgaaaaaatc gcagcaaagg aaatattatt atgggttaaa gagaggatat ttcagaaaaa
     2401 tgccctcccg ttcaagggga aggcgaggaa ttacagatag cggcacagat aagaggtggg
     2461 ttcggcaact tgcagatgaa actcactgtg accgggaaca ttaaacaccg aaccggcttc
     2521 atacacctgc cagtcggtcg catcaggcag taacacattc agcgcgccac tgattaccgt
     2581 catctcttcc ggctcagcgg tgctgaaggt gtattcgcct tcaaccataa cacccacgct
     2641 ggcgcgacca gtgctgctgc tggaaaagcc gattgatttc actttgccgg aaaagtactc
     2701 attactttga agcataaact ggccctgtct ggtagttgaa catctacttc actatagggg
     2761 ccagaggcgc tggctgtcac gcaaaattac accagtaatt ccgcagccag ccgtgcaatc
     2821 aatacgttag acagcaagac aggaacatcg agctgttttt gcagtaaatc gcgatgacgt
     2881 tggtgaaatc ctaaacaatc cagcatgatg acatcagccc cttttgccag taattctttc
     2941 cctgcatcaa tgattttttg ttctgaatca tgaatggggt tacccaatga aaataccggc
     3001 gatttctgca aaatttgcca tttttgcgcc tgaacgggca gcatctcctc aaccggaacg
     3061 ataaccccca cctgatgatc ttcaacaata gaggaaacca gtggaggcaa tattcgcgac
     3121 ggctcaagaa agatcgtatt acgcgcagtc atactactaa tgtttgctgt actcattaat
     3181 aaaatgacgt cataaccctg attatcgagc acttcaacca caccttgcag gtctcgctcc
     3241 actttacgac gcgaaacatg ggccagctgg ttgtcattta ataatgtgag aatggtgtct
     3301 tcgcctgctt ctggcgcgta ctccgccatc acttcttcac gacttaactt ccccagcagg
     3361 ctatgatggg aaatattatc ttcgtcaatg tattccgtca ggagcggcaa aacctcctgc
     3421 ataggtacaa tgccgatggt caggatcgcc aacgacgcac tcattgttca tccaccttac
     3481 ttttctttcg tcgttaatta ccgggcaagt gtgaagcacc actgctgaca cttacttgtg
     3541 ctacaaatga caaaaagcgt agcagcagac gtgcacggca taatgtaaag attccaaatg
     3601 attccagtaa tggattgtta ttgtttaata ttctaattat tagaaaaaca tgaattatga
     3661 aaaatgtgac gcagatccct tgtagtggca ctcagaatcc cttccgagca gtctgatgcg
     3721 gtcactcttt gtcagggtcc tcatagcgtt ttttcgcatc catcgcttcc tgagcggtcg
     3781 gatgttcact gatcaacgag tctggtttag gatgatcggc tctgagttgg taccaggtta
     3841 ccgtctgtcc tggctttcct ttttcaatcg tcactatata tgcttcacga ggataaggcg
     3901 gtttcgttgg catcgttctt ccttatttca cggaatgaac gttaagtata ggcgctctaa
     3961 aatcaacaat tgatcgtctg tgccagggcg ctacgaattt cagaaatcac ctggctgggt
     4021 tcgtttgttg cgtcgatgat aatatgcgca acttcgcgat atagcgcatc gcgttcttcc
     4081 agcacttcct gaacttcttc gctcagcggt tttcccgtta aggttggccg taaatcttct
     4141 tccggtgcag cttgcagtcg gttaaccagg actgatactg gcgcacacaa ataaaccacg
     4201 atcccgttat tttgcatgaa gtgacgatta aattccgtca gaataatgcc gccgcctgta
     4261 gcgataacgg tggatggcgc agttaccgct tccagcgccg ccgtttctct ggcgcgaaat
     4321 cccgcccact cttccctttc gacgatctcc gcgaccgtca tattgagctg tgattgcaac
     4381 cactgatcgg tatcgacaaa ccgacggtta agcgaatcgg caagggccat tccgaccgtt
     4441 gttttaccac agccccgagg cccgatcaga aaaagaggtt gtgtcatcgt gggttttccc
     4501 caataggtcg caatgcggcg aaagccggtg tcatgagaat agcgatcatt ccatcaaact
     4561 agtacaattt cgattgtaaa gaaaaaattc cacttaaagt gaaaatctca atacatccct
     4621 tactatacca ataaatattc aagaatgaag tgtaaataat aaattacatt tagccacgac
     4681 tacgttgcac ttccagccac cacttctcca gctccgcggc aaaggcctgg cggtcacgtt
     4741 gtgaaaggct atctggtccg ccggtctgga tcccactggc acgtaaggta tccataaaat
     4801 cgcgcatcgt caggcgctca cgaatggtcg ctggcgtgta acgttcgccg cgcggattaa
     4861 gcgccgctgc gcctttctcg atggcttcag cagccaaagg tatatctgcg gtgatcacca
     4921 aatcgcccgc ttcacactgc cggacaattt cgttatcggc aacgtcgaaa cctgccgcga
     4981 cgcgcagcgt acgaataaat cgcgatggcg gcacgcgtaa actctggttt gctaccagta
     5041 ccagcggcat ctgcatacgt tccgccgcgc gatacaaaat ctctttaatt acattgggac
     5101 acgcgtcagc atccacccaa attgtcataa agtcatcctt tgttgggtaa tcctctattg
     5161 tgtcgcgctt ttgccttccg gcatagttct gtttatgctt ctgccagcga ttatcaaaac
     5221 aatgaatttc acggcaggag tgaggcaatg gaaaagaaaa tcggttttat tggctgcggc
     5281 aatatgggaa aagccattct cggcggtctg attgccagcg gtcaggtgct tccagggcaa
     5341 atctgggtat acaccccctc cccgaataaa gtcgccgccc tgcatgacca gttcggcatc
     5401 aacgccgcag aatcggcgca agaagtggcg caaatcgccg acatcatttt tgctgccgtt
     5461 aaacctggca tcatgattaa agtgcttagc gaaatcacct ccagcctgaa taaagactct
     5521 ctggtcgttt ctattgctgc aggtgtcacg ctcgaccagc ttgcccgcgc gctgggccat
     5581 gaccggaaaa ttatccgcgc catgccgaac actcccgcgc tggttaatgc cgggatgacc
     5641 tccgtaacgc caaacgcgct ggtaacccca gaagataccg ctgatgtgct gaatattttc
     5701 cgctgctttg gcgaagcgga agtaattgct gagccgatga tccacccggt agtcggtgtg
     5761 agcggttctt cgccagccta cgtatttatg tttatcgaag cgatggccga cgccgccgtg
     5821 ctgggcggga tgccacgcgc ccaggcgtat aaatttgccg ctcaggcggt aatgggttcc
     5881 gcaaaaatgg tgctggaaac gggagaacat ccgggggcac tgaaagatat ggtctgctca
     5941 ccgggaggca ccaccattga agcggtacgc gtactggaag agaaaggctt ccgtgccgca
     6001 gtgatcgaag cgatgacgaa gtgtatggaa aaatcagaaa aactcagcaa atcctgatga
     6061 ctttcgcctg acgtcaggcc gccacttcgg tgcggttacg tccggctttc tttgctttgt
     6121 aaagcgccaa atctgccgat ttcaaccact cacgatagtg actcatttgt gggttcagcg
     6181 gcgcaacccc cacactaatc cgtaaagtta cctgtggcgt attcggcaag cgtaacgtgt
     6241 ttagcccttc acgcacccgt aacatggcgg taatggcgct ctcagctggc gtaccggaca
     6301 tgattactgc aaactcatcg ccgccaaacc gaccaatcac atcgctaccg cgcagggtaa
     6361 tttgtaactg tcgggtaagc gccacaatcg cttcatcgcc cacatcatga ccccaggtat
     6421 cgttgatgct cttgaaatgg tcgatatcga taatcagtaa cgttgcatcg cgattatgcc
     6481 gccgacagtt atcaaattca ttgcgtaaca tagtttccca atgacgtcgg ttatacacgc
     6541 ccgtcatgcc gtcgcgggta ctcatgacct gcaacctgcg tttatgttcc gccagtttgg
     6601 ttgccgtctg gtagctgacc cagccaaaca gcagaggata aacgacaata atgggaaggg
     6661 agagccacca ttccagcggc gcactattga acgacacggt aatgcccgta agctcgaggg
     6721 tgacaaggca ggaaaccacc atcaacacca gacccgcgac aaacagacgg gggccgcctg
     6781 cccccatcaa attcagacac ataatcatca acatagcggt ggaaggcagc acgtttacgc
     6841 ccattacgcc tacccacatt cccgctaata ctgcatcggt ttttaagttg taaatttccc
     6901 ggctaagcgg atcgacggcc ctgctcgcta tctgccaggc taaatgcggc cagacgaacg
     6961 cccagccgac caacactagc caccaccagc ccggcggcgg gtgcgaaacc agcgttgaag
     7021 caatcggtaa gaacatgcca gccaggccaa ccgcacgggg tagtctgacg cgacgggcga
     7081 agcgcagccc ggaccgctga tgttcgtttt gaggagttaa aggaggttcc tccccatgcg
     7141 ccgccgcttt tttgaaaaag ttttcatcat tcattatttt tgggaacatt cagaaacaat
     7201 tttcccaaat tatagagacg gggctaaagg cagaagtatg aaatttcggg tgagccactt
     7261 tcgtagctca cccgttagca ctatcaggta tcaggcggct ttcttgaggc aggcactcat
     7321 aaacttatta cgatcatcac ctttcagaga ttgttgcgtt gcttgattat tacattcgcg
     7381 catcttttgc tgctgtggcg tcaaactttt ttcaccaggc gcagacttgc tgttcttcag
     7441 gcaatcactc atgtaggtct tacgagcatc ccctttcaac gcctgcgccg tcgcctgctg
     7501 attacaggag gtcatacgct gttgttgtgg ggttaacgtt ctctcggcag cgccgactgt
     7561 ggttaaaaaa accagaccga aaagcaaggt aaccagtaat gtaattttca tagcaccatc
     7621 cctcttcatg ttttaaccat gagcgtatgc gcccgagatt tgccattaag tctggttgct
     7681 aacagcaaaa aaaccacccg gcagcgaaaa ttcactgccg ggcgcggttt tatttcagcc
     7741 ccagggcggc tttcatggtg tagaagagat cggtctggtc ggtcagtcca acgacattgg
     7801 cggcatgcgg gccatacgcc gcaatacgca actgactgcc ggtatgttct tgtgaatcct
     7861 cttcggagtt cccgtagctc atcaccatca ctgcgccatc tttggtattt agcgcctggg
     7921 tgaggcccgg agctttggta tccggcgcga caatctggct ggcgtgggcg tgatcagcgg
     7981 tgactatgac cagcgtgttg ccatcctttt tagcgaattc cagcgcccgt tgtacggctt
     8041 catcgagatc gaccgtctcg ccaatttgcc cacaaggatt cgcagcgtga tcctgtttat
     8101 cgattgacgc accttcaact tgcaggaaaa atcctttctc atttttactc aacaattcaa
     8161 tggctttgtc ggtcatctgc gccagggttg gtacactgtc attacgttgc ggattaggcg
     8221 tacaggtgac tgcgggctta tcgatattgc cgtggtacgt tgctttcggt cctagccagc
     8281 gcactggcat attgccgtca gcaaacagtc caagcagagg tttttgctga ttcgcttccg
     8341 tcaccgcatt tagcgaggcg gcatcgctga ccaactgata accacgcgcc tgtgcctgtt
     8401 cacgcagcgt ttttccctgc cattcaccgg cggttgccgt ttcagcaaag gtttttgcgc
     8461 caccgccaag cgtaacgtcg gcacgagcat taagcagctg ttcggtaatc gatccttttc
     8521 cgcctttttc cagagcgtta cccggacatt tttcactggt cgcgctcgga ccgtagcatt
     8581 tgcgcgaagt cacatgtgcc accagcgcag cgggcgtggc atcctgcaac tctgcggtag
     8641 aaacgttacc ggtcgccaga cctgcggctt ttgccatttc cagaatcgtt gggtgatctt
     8701 tttcgtgaat atcgacgccc agcgcgccgt tataggtttt gacaccggtt gaccaggcgg
     8761 ttgctgatgc agccgaatcg gtgacgtagt ccggtttgcc ggttttttta ttcagcgcat
     8821 agtgagtgta ttgcccggta agcggtaagg catcgatacc tttaaaaaag ccgcccgcac
     8881 cttcggcata attacgtgcg gctgtaattt ccgaatcccc catcccatcg ccaatcagca
     8941 aaataatatt ttttgcaggt ttatcgctaa gagaatcacg cagagcggca gtctgatcac
     9001 ccgttaaacg gcgagcaccg ccgggtgcag taatatcgcc ctgagcagcc cggttttcca
     9061 gaacaggcat ttctggtgtc cgggcttttg tcacaggggt aaacagtaac ggtaagagtg
     9121 ccagtgcaat agtgctttgt ttcactttat tttctccatg tacaaataca ttaaaaaata
     9181 aaaacaaagc gactataagt ctcggccgtg acaactttat gacagctgtt gaaaagatta
     9241 actttttact gacgaggatg cttcaataac ttctttacat aattgcgcag cagctccgta
     9301 tcgtcgtcag gaatgctggc atcaggcttt acctcgtaaa gagccccctc cacctgttca
     9361 atcaagcgct gttggtcatt ttgtgccata ttgcgaagca ttgcagtgac gataatctcc
     9421 aaagcttcta cctgcgcgca cagttctttc gactcttctt ctttttgggc aagctttaat
     9481 aacaactcag caatgagatt tttcatgtct gtatttcctt atccaacgta tggagaagtt
     9541 aacattatgt ttcacaactt catagtgtct ggaagtatta tttttatgac aaaggaaata
     9601 ctttgcgcaa atgttctttg tttgattacc agcgaaacgt ttctcatatt taaataatta
     9661 accacacaaa gaacaggcct ttaattatca acctttatta taaacaaaag ttatgaaata
     9721 tattctctgc gtttacttac aactaagaga aaaaactcac cgcatggcca tttaagccag
     9781 gaataaatta gcttcccgtt catcattttt gtgatctcgc gcacatcttt gtaataacgc
     9841 actttcttca ccattacgtt gcacgctttg tcagcagaag attgcaacat tctgtcagaa
     9901 ggccgcgaat cccccgtttt gtcgtggtaa tgtatgcact ttgtgttaga cgggataggt
     9961 ttttaagatg gaaaaactgc gggtaggaat cgtttttggt ggtaaatcag cggaacatga
    10021 agtgtctctg caatcggcaa aaaacattgt cgatgccatt gataaaagtc gcttcgacgt
    10081 tgtgctgctg ggcattgata aacaagggca atggcacgtc agcgatgcca gcaattatct
    10141 gctaaatgca gacgatcctg cccatattgc gttgcgccct tcggcgacta gccttgcgca
    10201 ggtgccaagt aaacatgagc atcagcttat cgacgcgcaa aacggtcagc cgttaccgac
    10261 ggtggatgtc attttcccga ttgtccacgg tacgctgggc gaagatggtt ccttgcaggg
    10321 aatgctgcgg gtcgccaatt taccgtttgt aggttctgat gttctggctt cagcagcctg
    10381 tatggataaa gatgttacca aacgtctgct gcgcgatgcc gggctgaaca ttgcgccatt
    10441 tattaccctg acgcgcgcca atcgtcacaa cattagtttt gccgaagtgg agtctaaact
    10501 gaggttacca ctgtttgtaa aaccggctaa tcagggctct tctgttggtg tcagcaaagt
    10561 aaccagtgaa gaacagtacg caattgccgt cgatctggcg ttcgaattcg atcataaagt
    10621 gatcgttgag caagggatca aaggtcgtga gatcgaatgc gcagttctgg gcaacgacaa
    10681 cccgcaagcc agcacctgtg gcgagatcgt actcaccagc gacttctatg cctacgacac
    10741 caagtacatt gacgaagatg gcgcgaaagt ggtagttccg gcagccattg cgccagaaat
    10801 caacgataag atccgggcga ttgccgttca ggcttatcaa acgttgggat gcgcaggcat
    10861 ggcgcgtgta gacgtgtttt taaccccaga gaacgaagtg gtgatcaacg agatcaacac
    10921 actgcctggc ttcaccaaca tcagtatgta tccgaagctg tggcaagcca gcggtctggg
    10981 ttacaccgat ctgatcacac gtttgattga gctggcgctg gagcgtcacg ctgcggataa
    11041 cgcattgaaa accacaatgt aatattttaa cgcccgataa tattcttatt gggcgttttc
    11101 agtttcttca tcgcgacgac gaataatcaa ccccgccagc cagaaactaa tcacccacgt
    11161 caccagaccg acggcatagg tctgccaacc ttttgcttca aatcccagta accccaccgc
    11221 gccattaaga atgaatataa ggccaatggc gaacgcatag tagtgccagt cacggcggat
    11281 ttttacaggc agattcatgg cagctccgtt aacgaaaaac aacatccttg catattctgt
    11341 aacaaccgtc tgtggtgtag acaacatttt cgtaattgtt aataaattta acttaaggtt
    11401 gccatt
//
LOCUS       DAJTMC010000132        11405 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.132, whole genome shotgun sequence.
ACCESSION   DAJTMC010000132 DAJTMC010000000
VERSION     DAJTMC010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11405
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.132"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            <1..921
                     /gene="gap"
                     /locus_tag="OO130_003139"
     CDS             <1..921
                     /gene="gap"
                     /locus_tag="OO130_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCR5366447.1"
                     /translation="DVVAINDLTSPKILAYLLKHDSNYGPFPWSVDFTEDSLIVDGKS
                     IAVYAEKEAKNIPWKAKGAEIIVECTGFYTSAEKSQAHLDAGAKKVLISAPAGEMKTI
                     VYNVNDDTLDGNDTIVSVASCTTNCLAPMAKALHDSFGIEVGTMTTIHAYTGTQSLVD
                     GPRGKDLRASRAAAENIIPHTTGAAKAIGLEIPELSGKLKGHAQRVPVKTGSVTELVS
                     ILGKKATAEEVNNALKQATTNNESFGYTDEEIVSSDIIGSHFGSVFDATQTEITAVGD
                     LQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            complement(963..2403)
                     /gene="aldA"
                     /locus_tag="OO130_003140"
                     /pseudo
     CDS             complement(963..2403)
                     /gene="aldA"
                     /locus_tag="OO130_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
     gene            complement(2558..3400)
                     /locus_tag="OO130_003141"
     CDS             complement(2558..3400)
                     /locus_tag="OO130_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HCR5366448.1"
                     /translation="MNITPFPTLSPATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIL
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALFNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFINQLQGLWPVERYLSLLTGELPR
                     LRDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKHDAVLIEAMESRSLRQKLPVCEPLV
                     CKRA"
     gene            complement(3672..7574)
                     /gene="hrpA"
                     /locus_tag="OO130_003142"
     CDS             complement(3672..7574)
                     /gene="hrpA"
                     /locus_tag="OO130_003142"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HCR5366449.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DPALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            7775..8380
                     /gene="azoR"
                     /locus_tag="OO130_003143"
     CDS             7775..8380
                     /gene="azoR"
                     /locus_tag="OO130_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HCR5366450.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            complement(8431..9747)
                     /locus_tag="OO130_003144"
     CDS             complement(8431..9747)
                     /locus_tag="OO130_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HCR5366451.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDLNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSTTFEQRRLVHRLILATVMACCG
                     FLLYPLKFSFIRPEVSGVTGWPFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAER
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKPDQRRIK
                     IALPYVVGAGSCIVLMELMVMIQLWWPVWLCWPVLSLLIIGRGYGGLGAITTGKDSQG
                     KLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKITAGVYLGAFPRHIPAQNAVLDVTF
                     EFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSA
                     LVVAAWLLCYGHCKTVDEAISYIRARRSHIVLKEDHKAMLKLWENR"
     gene            complement(9737..11405)
                     /locus_tag="OO130_003145"
                     /pseudo
     CDS             complement(9737..11405)
                     /locus_tag="OO130_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415928.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
BASE COUNT         2841 a         3097 c         2714 g         2753 t
ORIGIN      
        1 gacgttgtcg ctattaatga tctcacttcc ccaaaaattc tcgcctacct gctgaaacat
       61 gattcaaact acggaccatt cccctggagc gttgatttta cggaagattc acttatcgtt
      121 gatgggaaaa gtatcgcggt ttacgccgaa aaagaggcta aaaatattcc gtggaaagcg
      181 aaaggtgcag aaatcattgt cgaatgtact ggcttttata cctccgccga gaaatcgcag
      241 gcgcatcttg atgctggtgc gaagaaggtg ttgatttccg cccctgccgg tgaaatgaaa
      301 actatcgttt ataacgtcaa tgacgacact ctggatggca acgacaccat tgtttccgtg
      361 gcgtcatgca ccactaactg tcttgcgccg atggccaaag ccttgcatga cagtttcggg
      421 atagaagtcg gcacgatgac gaccattcat gcctatactg gcacccagtc actggtggat
      481 ggcccacgtg gtaaagattt acgtgcttca cgcgcagcgg cagaaaatat cattccccac
      541 actacggggg cggcaaaagc cattggtctg gagatcccgg aactgagcgg caaactgaaa
      601 ggtcatgcgc aacgcgtgcc ggtgaaaaca ggttcggtca ctgaactggt atcgattctc
      661 ggaaaaaaag cgactgccga agaggtgaat aacgcactta aacaagcaac caccaataac
      721 gagtcatttg gttataccga tgaagaaata gtctcttccg atatcattgg cagccatttc
      781 ggttcggtgt ttgatgccac gcaaacggaa attaccgccg tgggcgattt acaactggtg
      841 aaaacggtcg cctggtacga taacgaatat ggcttcgtca cgcagcttat tcgcaccctc
      901 gaaaaattcg ctaaactctg acgcgcacag gcggaggaaa aaacctccgc ctcttttact
      961 cattacgact gtaaataaac cacctgggtc tgcagatatt catgcaggcc atgtttacca
     1021 tctgcgccgc caataccgga tttacgccat ccggcgtgga agccttgcat agcttcgaac
     1081 ttttcacggt tgatgtaagt ttcaccaaac ttcagccctt taatggcttt catcgcgacg
     1141 ttcagatttt gggtatagat ttgatgaggt caggccgtaa tcactgtcat tagccattga
     1201 gatagcctct tccagcgtgt caaatgcgac caccggcaga accgggccaa aggtttcctc
     1261 atgcataatc gacatttcct ggcgaacatc cagcagcaat gtcggcggat aataatatcc
     1321 tttcccctct accgctttgc cacccaacgc cactctcgcc ccttcttcta ctgcgcgcgc
     1381 cactttttgc tcaacccttt ccaacgccgc ggcgttaatc aacggcccca tcgcaatgtc
     1441 gttgcgttca gcggggttac caaattgaac cgcctgcatc gcttcaccca gccgattgac
     1501 gaactgatca taaatgcctt tctgtacata aacacgttct gcacagttac acacttgccc
     1561 actattaatg acgcgtgaat caacgatggc tttgactgcc agttcaagat cggcatcgtc
     1621 cattacgata gctggtgctt taccccccaa ttccagacac actttggtga tgtttttcgc
     1681 cgcagtcgcc atgatcttct cacctgcaga gacgctgcct gtcatactga ccattgcgac
     1741 ctttgggtta cccgccagtt cttgcccaac ggtttcacca cgccccagta caaggttaaa
     1801 cacgccgcgc ggaaggccta tttcatcgac gattttggcg aatgcaatcg cattgtttgg
     1861 cgtaaattca ctaggtttaa tgacgatggt attaccggtc aaaagagcgg gagccatttt
     1921 gcgggcaatg aggaagaacg ggaagttcca cggcagaatg ccggtagtca caccaagcgc
     1981 acgtttaaac aaaagaatat tttctcctgg acgatcgctt tgaataatct cgccctcgta
     2041 acgccgtgcc cactccgcca tgtaatcgat atagtcggca gtaaaagcca cttcgacttc
     2101 agccagctgc tggatcttgc ccccttcttc aacaatcagc gcactgattt cactggcgcg
     2161 ttcgcggatc ccggcggaga ttttgcgcaa ccaactggcg cgttcaatag caggcaacgc
     2221 ttcccattct ggttgtgcac gttctgctgc atcgattgcc ttacgggcat cctcggcctg
     2281 accatcgggt atgcgggaaa tgacagcctc tgtagcaggg tttaccacat caatccatgc
     2341 gtctccacgc caggtaacaa actgtccatc gatatacata ggatgttgaa cgggtactga
     2401 catgggcgac tcctgtgatt tatatgtttg ttcttggtaa attcattgtt aattaacatc
     2461 aatcatcata gagacggcat cgtgaaagga caattctgtg aagggcttca taaaaacaag
     2521 tgtaaaagga ataaaaggca gaaataaaag tcaggagtca tgcccgtttg caaacaagtg
     2581 gttcacaaac gggcaatttt tgacgtaacg agcgactttc catcgcctcg atgagcaccg
     2641 catcgtgttt aagcgtttgc catgcatgga tgacttctgc cggaaaatca acgtgaacga
     2701 taaaatctcg cccgcgggga ccgtagccat cgctatcatc gcgtaaacgc ggcagctcgc
     2761 cagtgagtag tgagagataa cgctcaaccg gccataatcc ttgtaactga ttaataaaga
     2821 ttacactgtc tgcgttattt cctaactgag gaacgaatcc gggataactt aaccagcgtg
     2881 gtgcatcggg attgtcccca gtcatacggc ggaacgtcgc catcgtgcgc cgctgcatgg
     2941 tggggtcctg aacaacgata gccgtatgaa ctcgttctac ggcctgatta aatagcgcga
     3001 tgctaaagcg tgcgttttca ccgcagtttg ttgactggtc ttcaatccag attttttcat
     3061 gcggaatgtg ccagaactga tgagcgatat ccgccaggat ggtcgcttct gctctgccag
     3121 tggtgcggat agtgttgtag tgcggatgct gtgcgatggc gctatacaaa aaagttgtcg
     3181 agtgaccgat accaccacta atcagtaaag gaatttgctg atcgcgagca atcttacatg
     3241 ccgcgtcgat agtcggcata accgcattgc ctgcaaggat cacgcaatcg gcctgatacg
     3301 gcacctcacc ggagaaatca tcctgcgcca gccactgtcc gataacattt atggcatcta
     3361 tagttgccgg cgaaagcgtc ggaaacgggg taatgttcat ggttcctcct tatcgttccc
     3421 ttaagaatac tgcgtgagat aatagtgaga agctgagcac tctgaaagga ggaagtcgta
     3481 tgtccttaac cgccactccg caagcctgac atatctttta aggaaatgct gtagataaac
     3541 attattcatc ttgcagcaga gaattgtatt tttctgaatt cctgaatttt tacccttacc
     3601 gttttagaat taataaaaaa cccggcgcta aatgcttacc gggttttaga tttattaggc
     3661 aaatagcagg gttaaccgct aatctgctcc atcgcctgca aaatacgctt atctgaaatc
     3721 ggataaggcg taccaagttg ttgagcgaag taactaacgc gcagctcttc tatcatccaa
     3781 cggatctctt tcacgtcttc atcctcacga cgtgcaggcg gcagtttgtt gatccattgc
     3841 tgccacgcct gctggacgtt ttcgactttc agcatctgtg cacgatcgcg atgtggatca
     3901 accgccagtt tttccagtcg tttttcaatc gcctgcaaat atcgcagcgt gtcgcccagc
     3961 cgtttgaagc cgttaccagt gacaaaaccg cgatatacca acccgcccat ctgcgcttta
     4021 atgtcagaaa gccccagtgc catggtcata tccacccgcc ctttcagacg tttgttgata
     4081 ttgaacactg ccgtaagaat ttgctcgacc tgcttcgcaa tatctaccac cgtgtcgttc
     4141 agttcggcac gcactttctc atgcagcgca gcaaagcctt cttccgtcca gaccgggcca
     4201 ccattggcgt cgatcagttg atccacaccg caggagatac agtcgtcgat cagctccagc
     4261 actttgccat acgggttgaa gtacagtccc agcttggctt tgttcggtaa cttttcatgt
     4321 aaatacttga tcggcgaggg aatatttagc agcagtagac ggcgaagacc attccacatt
     4381 gcctgttttt gctccagcgg gttatcaaac agtttgatcg ccacgctgtc gcgttcatcc
     4441 accagcgccg gccacgcctt cactttatag ttgccacgct tctgttcgta actttccggc
     4501 agctgaccaa aactccagat atgtaagccg ctctgctcga taccgtcatc cgccaccgcg
     4561 gacagcgttt cctgcacttt gcctttcagc gcatctttca gatcttgtag cgagcgccct
     4621 tcttttagct tcttgttttt gtcatccacc acgcggaagg tgattttcag gtgatcgggc
     4681 acctgatccc agtgccagtc ttcgcggtca acggtaacgc cggtcatccg ccgtaactcg
     4741 cgctcaaggc tgtcgagcaa cggtaactcc agcggtgtga cgcggcctaa aaacgcttcg
     4801 gcgtagtttg gtgcaggcac aaaattacgg cgtaccggtt tcggcaacga tttaatcaga
     4861 gcaatcacca attcgcggcg cagaccgggg atctgccatt caaacccgct ttcctcaacc
     4921 tggttaagta acggcagcgg aatatgtacg gtcacaccgt ccgcgtctgc gcctggctca
     4981 aactgatagc tcaaacgcag cttgagattg ccctgatgcc agaagttcgg gtaatccagc
     5041 ttgctgattt tttccgcccc ttctttgatc aacatgctct tttcaaagtt gagcaaatca
     5101 ggcgtttcgc ggctgacttt tttccaccag ctatcgaagt gacgagcgga gattacatcg
     5161 tggctgatgc gctggtcgta gaactcaaac agcgtttcgt catcaaccag aatatcgcgg
     5221 cgacgtgatt tgtgttccag ctcttccact tcggcccgca gtttcaggtt ttcacggaag
     5281 aatgcgtgac gcgtctgcca gtcaccttcc accagcgcat ggcgaataaa gagttcacga
     5341 cataacgccg gatcgatctg gctgtagttg accttgcgcg cggcaacaat cggcaaacca
     5401 taaacagtga ctttttccgt tgccatcacc gcgccctgcg cccgttccca gtgcggttcg
     5461 ctgtaggtac gtttaatcaa atgctgagcg acaggctcca cccattccgg gtcgatacgc
     5521 gcagcaatgc gcccccacag gcggctggtt tctaccagtt ccgccaccat tacccatttc
     5581 ggcggttttt tgaataaacc agaaccgggg aagatggaga aacgcgcgtt acgtgcgccg
     5641 gtatattctt gtttatcggc atctttcatg ccgatatggg aaagtaaacc cgtcagcaac
     5701 gcaatgtgaa tttcgcgata ctccgccggt tcgctgttaa ccggaatgcc aagttctttc
     5761 accacctgac gcaactgggt gtagatatcc tgccattcgc gcacgcgcag atagttgaga
     5821 taatcggtac gacacaggcg acggaaggcg tttgaagaaa gcgccttttg ctgctcgcca
     5881 agataattcc acagatttac aaacgcgagg aagtcggatt ctttgtcgtg gaagcgacga
     5941 tgtttttcgt ccgatgcctg ctgtttgtcc atcggccgct cacgcgggtc ctgaatggag
     6001 agcgcggacg tgataatcat cgcctcacgc acgcagccat gtttctgcgc ttccagcacc
     6061 atacgcgcca gacgaggatc gaccggcagt tgcgagagct ggcgaccgag cggcgtcagt
     6121 ttataggcgc tggcctgttc atccgtggtg atcgcgccca gttcttcgag cagacgcacg
     6181 ccatcctgga tattgcgttt atccggtgct tcgacaaacg ggaacgcagc gatatcgccc
     6241 agccccagcg cggtcatctg caaaataacc gaggccaggt tggtacgcag aatctccgga
     6301 tcggtaaact ccgggcgcga gaggaaatcg tcttcggaat agagacgaat acagatccct
     6361 tcggacacac gaccacaacg gcctttacgc tgattggcag acgcctgtga aatgggttca
     6421 atcggcaaac gctgcacttt ggtgcgatag ctgtagcggc tgatacgcgc cgtaccgggg
     6481 tcgataacgt atttaatccc cggtacagtc agcgaggttt ccgcgacgtt ggtcgccagc
     6541 acaatgcgtc gcccgctgtg cgactggaac acgcggttct gttcgctatt cgaaagccgc
     6601 gcataaagcg gcaagatttc ggtatggcgt aagttcagct tgttcagcgc atcggcggta
     6661 tcgcgaattt cccgctcgcc gctcataaag atcagaatgt cgccagggct ttcctggctc
     6721 agttcgtcta cggcgtcaaa aatcgcctgc aactgatcgc gctcggtgtc atcggcttct
     6781 tcaacaatcg ggcgatagcg cacttccacc ggataggtcc gaccggagac ttcgataatc
     6841 ggcgcattat taaagtggcg agaaaagcgt tccgggtcga tagtcgcgga agtgataatg
     6901 atttttaggt caggacgccg cggcagcaac tctttcaaat agccgagcag aaaatcgata
     6961 ttcaggctgc gttcgtgtgc ttcgtcaata atgatggtgt cgtactgcat cagcaggcgg
     7021 tcttgctgga tctccgccag caggataccg tcggtcatca gcttgaccat cgtgttatca
     7081 cttacgtgat cgctgaaacg cactttgtaa ccgatgcaac cgcctggctc cgttttcagc
     7141 tcttccgcaa tacggttcgc caccgttctt gccgccagac gacgcggctg ggtatggccg
     7201 atcagtcctt taatcccgcg ccccagctcc atacagattt tcggtagctg ggtcgtttta
     7261 ccggaaccgg tttccccggc gacgatcacc acctggtgat cacgaatcgc ttcgagaata
     7321 tcctgttttt tctgactaac tggcaaattg tccggataag tgatttccgg tcgtgccgct
     7381 tcacgcagca ggactttccc tgccgcctgg tcaatctctt tcgccatctc ctggaaaatg
     7441 gcctgttgtg catcaggatt tttaaccttc ttaacaccat gcagacggcg agaaaaacgc
     7501 agtctgtcgc gcagcatcaa cgaatccagc cgctgctgca aagcagtaaa ggtcaatttt
     7561 tgttgttctg tcataacgtt agtgggcagt agtctgcctg attaattttc ttttaaatga
     7621 tagatataga gtaccacatc gcggctttct atgcgttgtt caataaattc gaacataggc
     7681 ttcgatttat tgcgctatct ctgtggcagg attgtgaata aagtgtcagc aagcaacggg
     7741 gcatcgccaa ttcaaacatc tataaggaaa caccatgagc aaggtattag ttcttaaatc
     7801 cagcatcctg gcagggtact ctcagtctaa tcagttgtcc gattattttg ttgaacaatg
     7861 gcgcgaaaag cactccgctg atgaaatcac cgttcgcgac ctggctgcaa atccgattcc
     7921 ggtactggat ggcgaactgg ttggcgctct gcgtccgagc gatgcgccgc tgactccgcg
     7981 tcagcaggaa gctctggcgc tttccgatga gctgattgcc gagctgaaag cccacgacgt
     8041 tatcgttatt gcggcaccga tgtataactt caacatctca actcagttga aaaactattt
     8101 tgacctggtt gcacgcgcag gcgttacttt ccgctatacc gagaacggtc cggaaggtct
     8161 ggtaacgggt aaaaaagcca tcgttattac cagccgcggt gggatccaca aagatggacc
     8221 aacggacctg gtgacgccgt atctgtccac gttcctcggc tttatcggca ttaccgatgt
     8281 gaaatttgtc ttcgccgaag ggatcgcata cggtccggaa atggcagcga aagcgcagtc
     8341 tgacgcgaaa gcggccatcg acagcattgt tgctgcataa ccggacacga tcttgatgtg
     8401 gactgtagtt cagtccacat ctcaatccac ttacctgttt tcccataatt tcagcatcgc
     8461 tttgtgatct tccttaagca caatatgcga acgtctggct cgaatataac taatcgcttc
     8521 atcaacggtt ttacagtgtc cgtaacataa caaccatgcc gccactacca gcgcactgcg
     8581 cgataatccc aacgcgcaat ggaccagaac gctgccttgc tcttcgcgta atgtttccag
     8641 catcgccacg gcctgtcgga gctccccctc ttccggaacc accagatcca gcatcggtac
     8701 acagaaatag agtcgatctt ttgtcgctcg tccccgaggg aattcaaagg tgacgtccag
     8761 aaccgcattc tgtgccggaa tatgtcgtgg aaacgcccct aaataaacac cagcagtaat
     8821 tttgctcacc ggctccaggc gacgacaaaa ccaacgcata gacagccaca tcccgatgcg
     8881 ccagggcaat gtcagccagt aaacggcggg cgggagtttc ccctgactat ctttccctgt
     8941 tgttatcgcg ccaagcccac cgtacccacg gccaataatg agtagcgata atactggcca
     9001 acataaccag actggccacc ataactgaat catcaccatt agctccatca acacaatgca
     9061 cgagcccgcg cctacgacat atggcagtgc tattttgatt cgacgttgat caggtttctg
     9121 ataattccaa cgacgatcga cgggcaccat ccagtcaatc aacataccta ccgccagccc
     9181 tgtgatgaca tcaataaaat gatgctgcca ggtcgtcaga gtagaaatag cgatgagtaa
     9241 aaaccatccg ccacagactt tacgccacct ctcagccaga tgctgacgaa agtgacgcca
     9301 aagtagccag cagagaataa tatgcagcga aggagactgg ttataaggca gatcaaacag
     9361 ttcaagttgc gaaaatggcc atcccgtcac cccactcact tcaggacgga taaaactaaa
     9421 cttcagcgga tagagcaaaa aaccgcagca ggccattacc gttgccagaa taagccggtg
     9481 gacaagtcgg cgctgttcga atgtcgtgct acaaacgaac agcgaaaatc catataaaag
     9541 atccagactc cagtaaggaa caatagtcca gggaagaaaa gggatcgccg tttcccaacc
     9601 gaatacctga ctggggatat catggctgtt aaggtcctga accgcggtga actgattaag
     9661 agatccatag gtgaagaaaa aaaacggggc cagcaacaat aaccagccag cgccttgtag
     9721 caatacgttc cgacgttcag ttatcacgac gcaccgccat cgaaaccgta aaaatacccc
     9781 actcatcaat ccgttgtgtg catttatcaa atccggcatc acgcacgagt gaatccatct
     9841 ccccttgcga acgcacgcgc attacccacg gtttaccatc tttatgactg gttaacaccc
     9901 cggcaatcat ctccagttga gggtgccacg gctgcccggt gtagatgaga atgccgaccg
     9961 gttcgatggc attggcaaga cctgcgagtg agttttttac ctgctcattt tcgggaaaaa
    10021 gctcatacag tccagagaca attgccagcg taggccgcgg agttaacgcg ctgagttcct
    10081 ccgggttaaa cgcatcgccc tgttcaaaac gcacccgccc agacattccc cgttgggcaa
    10141 tcatctcttg cccctgtgca acatttaact cgctgtaatc acgtaacaaa atatcgctta
    10201 cggcaggctc gtttgccagc gcatccagta catagcgccc atgccctgcg gcaatgtcaa
    10261 ccacgcggac ggctaaacct ttggcgtgga gatcggcaac ggcttgttta atcagtattt
    10321 gtaaatgggt tttgcgctgg cgaataccgc gccagccaac actgttcagg tagattttgt
    10381 cgattaagcg cccgaatgcg ttactacctt gcggttgatt ttgatagaca taatccagcg
    10441 aactgcctga atcaaagccg gtgctcattc cgagatgcag gcccgcagag tgcgtcccga
    10501 acaatttcat cgcctttcgc ataaagcgat acgctaaatc aaccggcgat aatagcacgg
    10561 gtccaccaga aagtagccgc cattgatccg ctgatggtcc tgtgcagtct tcatgctgat
    10621 aatcaaattt ttgcgatttg ttagcatata aacgactaat aaagctttgc attttttcaa
    10681 atgccagcgc cctgttctct tcacccaacg tgtcgtgata aaagcctggc agcagatgca
    10741 gctctttcag agggctacgt aatctctgat aaaaatcaat ctgcggttgg cgatgcacca
    10801 catagtcatc gcctgatatc agaagttgcg tggggagcgt aatcgccgcc gcatctctaa
    10861 taatacgttc agacgtttta tagagatcga gcaagatatt gacggcaatc gcccgtgtga
    10921 tcagcggatc attattgaaa ctcgccaccc gttgccgatc gtgggtcaaa tagcgtcctt
    10981 tcacatagga attaataaaa aacagaccac gcagacgatg ccataacgcc agcgcaggac
    11041 gtgccagcgg cacatacaat ttaaccttaa aggccggaga agccagcacc agcccgcgaa
    11101 ttgcaggcgc ataatcatga acctatgtgg caaccagcac tgcgccgacg ctttgcgcga
    11161 tcacaaccac ctcttccagt ccaacctagc tgtcgctggc agcaaaacgg acaaattcat
    11221 cgacatcctg cactgaacgc gcaagagatg ggctataacc acgcggcccc gaagtttttc
    11281 catgcccccg agcatcccat gcataaaaag cagtatctgg catcgccagt tcatcaacga
    11341 tatgttgtag acgaccagaa tgttcatggc cacgatgaaa taagacaatg acctttttcg
    11401 cccct
//
LOCUS       DAJTMC010000133        11296 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.133, whole genome shotgun sequence.
ACCESSION   DAJTMC010000133 DAJTMC010000000
VERSION     DAJTMC010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11296
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.133"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            289..1266
                     /gene="csiD"
                     /locus_tag="OO130_003146"
     CDS             289..1266
                     /gene="csiD"
                     /locus_tag="OO130_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation induced protein CsiD"
                     /protein_id="HCR5366452.1"
                     /translation="MNALIAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKPLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            1286..2554
                     /gene="lhgO"
                     /locus_tag="OO130_003147"
     CDS             1286..2554
                     /gene="lhgO"
                     /locus_tag="OO130_003147"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HCR5366453.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNDIRYDNCGKMLIATSDLEMERM
                     RALWERTAANGIEREWLNAEELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVVRTRQGGEYEASTLISCSGLMANRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFNDTLEILGSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRATRSVDALHAAFNQ"
     gene            2577..4012
                     /gene="gabD"
                     /locus_tag="OO130_003148"
                     /pseudo
     CDS             2577..4012
                     /gene="gabD"
                     /locus_tag="OO130_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
     gene            4026..5292
                     /gene="gabT"
                     /locus_tag="OO130_003149"
                     /pseudo
     CDS             4026..5292
                     /gene="gabT"
                     /locus_tag="OO130_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417148.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
     gene            5530..6891
                     /gene="gabP"
                     /locus_tag="OO130_003150"
     CDS             5530..6891
                     /gene="gabP"
                     /locus_tag="OO130_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005114089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HCR5366454.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYVPVKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTDLLAIGIICTVPIMAA
                     GIS"
     gene            6950..7612
                     /gene="csiR"
                     /locus_tag="OO130_003151"
     CDS             6950..7612
                     /gene="csiR"
                     /locus_tag="OO130_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HCR5366455.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGSDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(7613..8062)
                     /gene="kbp"
                     /locus_tag="OO130_003152"
     CDS             complement(7613..8062)
                     /gene="kbp"
                     /locus_tag="OO130_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HCR5366456.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(8146..8304)
                     /locus_tag="OO130_003153"
     CDS             complement(8146..8304)
                     /locus_tag="OO130_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HCR5366457.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            8487..8786
                     /locus_tag="OO130_003154"
     CDS             8487..8786
                     /locus_tag="OO130_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5366458.1"
                     /translation="MTELAQLQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLIDSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            8796..9320
                     /gene="ygaP"
                     /locus_tag="OO130_003155"
     CDS             8796..9320
                     /gene="ygaP"
                     /locus_tag="OO130_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HCR5366459.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDRIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(9367..9771)
                     /gene="stpA"
                     /locus_tag="OO130_003156"
     CDS             complement(9367..9771)
                     /gene="stpA"
                     /locus_tag="OO130_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HCR5366460.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            10439..10888
                     /gene="alaE"
                     /locus_tag="OO130_003157"
     CDS             10439..10888
                     /gene="alaE"
                     /locus_tag="OO130_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HCR5366461.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(10925..11269)
                     /locus_tag="OO130_003158"
     CDS             complement(10925..11269)
                     /locus_tag="OO130_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaC family protein"
                     /protein_id="HCR5366462.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
BASE COUNT         2664 a         2857 c         2982 g         2793 t
ORIGIN      
        1 gtttactaac ggtcattttg cagtgaagcc atttactgtt ttttatcgac cagataatct
       61 gttctctaat gttaactccc cctaacctgt tgctttagtt attcatttcc tgtctcactt
      121 tgccttaata ccctacgtta aatgttacta atttgttgtt tttgatcaca ataagaaaac
      181 aatatgtcgc ttttgtgcgc atttttcaga aatgtagata tttttagatt atggctacga
      241 aatgagcatc gccatgtcac cctacatctc ataagaggat cgcttctgat gaatgcactg
      301 atcgccgtac aaaataacgc tgtcgattca ggccaggact atagcggatt cactctcatc
      361 ccgtcggcgc aatccccgcg tctgctggaa ctcaccttca ccgaacagac gaccaaacag
      421 tttctcgaac aggttgccga atggcccgtg caggcgctgg agtacaaatc gtttctgcgt
      481 tttcgggtag gcaaaattct tgacgatctg tgtgcgaatc agctgcaacc attgctgttg
      541 aagcccctgc taaaccgtgc tgaaggtgcg ctgttgatca atgcggtggg tatcgatgat
      601 gtggcgcagg cagatgagat ggtgaagctg gcaacggcgg tagcgcatct gattggtcgc
      661 tcaaatttcg atgcgatgag cggtcagtat tacgcgcgtt tcgtggtgaa aaatgtcgat
      721 aactcagaca gctatctgcg tcagccgcac cgcgtgatgg aactgcacaa cgacggcacg
      781 tatgtcgaag agatcaccga ttacgtgctg atgatgaaaa tcgatgagca aaacatgcag
      841 ggtggaaatt cgttgctgct gcatctcgat gactgggaac atctggacca ctatttccgc
      901 catccactgg cgcgtcgccc gatgcgcttt gccgcgccgc cgagcaaaaa cgtcagcaaa
      961 gatgttttcc atccagtgtt cgacgttgat caacagggtc gcccggtgat gcgctatatc
     1021 gaccagttcg tccagccaaa agattttgaa gaaggcgtgt ggttgagcga gctttccgat
     1081 gccattgaaa ccagcaaagg cattctttct gtacccgttc ccgttggcaa attcctgttg
     1141 ataaacaacc tgttctggct gcacggtcgc gaccgcttta ctccgcaccc ggatctgcgc
     1201 cgtgaactga tgcgtcagcg tggctatttc gcttacgcca ctcaccacta ccagacgcat
     1261 cagtaagcgc aaaggaattg agcggatgta tgattttgtg attattggcg gcggcatcat
     1321 cggcatgtcg accgccatgc aactgattga tgtttatccg gatgcccgca ttgcgttgct
     1381 ggaaaaagag tccggcccgg cctgtcacca gacgggccac aacagcggcg tgatccatgc
     1441 cggggtctat tacacgcccg gcagcctgaa ggcgcagttt tgcctggcgg gaaaccgcgc
     1501 caccaaagcc ttttgcgatc aaaacgacat tcgctatgac aactgcggca agatgctgat
     1561 cgccacgtcc gatctcgaaa tggaacggat gcgcgcgttg tgggaacgca cggcggcgaa
     1621 cggtatcgag cgcgagtggt taaacgccga ggaactgcgc gagcgcgaac cgaatatcac
     1681 cgggctcggc ggcatttttg tgccgtccag cggcattgtc agctatcgcg aagtcacagc
     1741 ggcaatggca aaaatcttcc aggccagagg cggtgagatt atttataacg ccgaagtcag
     1801 cgccctcagt gagcataaaa acggcgtggt cgtccgaacc cgtcagggcg gcgaatatga
     1861 agcatcgacg ctgattagct gttccgggct gatggctaac cggctggtga aaatgctcgg
     1921 cctcgaaccg ggttttatta tctgcccgtt ccgtggcgag tatttccgcc ttgcgccgga
     1981 gcataaccag attgttaacc atctgattta ccccatccct gacccggcaa tgccgttttt
     2041 gggcgtgcat ctcacccgca tgatcgacgg cagcgtgacc gtcgggccaa acgcggtgct
     2101 ggcttttaaa cgcgaaggct atcgcaagcg cgacttctca tttaacgaca cgctggaaat
     2161 tttaggctcg tcggggattc gccgggtgct gcaaaaccat ctacgctcag gactgggcga
     2221 gatgaaaaac tcgctgtgca aaagcggcta tctgcggctg gtgcaaaagt attgtccccg
     2281 gctttcgtta agcgatctcc agccgtggcc cgccggtgtg cgggcgcagg cggtatcgcc
     2341 ggacggcaag ctgattgacg attttctgtt tgtcaccacc ccgcgcacga tccacacctg
     2401 caatgcgccc tccccggcag cgacatcagc aattcctatt ggtgcgcata ttgtcagcaa
     2461 ggtacaaacg ctgttggcaa gccagagtaa ccccggacgc acgctgcgag cgacacgtag
     2521 tgtggatgcc ttacacgccg catttaatca ataacctttg aaaacaggac gtaacaatga
     2581 aacttaacga cagtaactta ttccgccagc aggcgttgat taacggggaa tggctggacg
     2641 ccaacaatgg cgggatcatc gacgtcacca atccggcgaa cggcgacaag ctgggtagcg
     2701 tgccgaaaat gggcgctgat gaaacccgcg ccgctatcga cgccgccaac cgcgccctgc
     2761 ccgccaggcg tgcgctcacc gccaaagaac gcgccaacat tctgcgcaac tggttcaatt
     2821 tgatgatgga gcatcaggac gatttagcgc gcctgatgac cctcgaacag ggcaaaccac
     2881 tggccgaagc gaaaggcgaa atcagctacg ctgcctcttt tattgagtgg tttgccgaag
     2941 aaggcaaacg tatttatggc gataccattc ccggtcatca ggccgataag cgcctgattg
     3001 ttatcaagca gccgattggc gttaccgcag ctatcacgcc gtggaacttc ccggcggcga
     3061 tgattacccg taaagccggt ccggcgctgg cggcaggctg caccatggtg ctgaagcccg
     3121 ccagtcagac accgttctct gcgctggcgc tggcggagct ggcgattcgc gcgggcattg
     3181 cggctggcgt gtttaacgtg gtcaccggtt cggcaggcgc ggtcggtaat gaactgacca
     3241 gcaacccgct ggtacgcaaa ctgtcgttta ccggttcgac cgaaattggc cgacagttaa
     3301 tggaacagtg cgcgaaagac atcaaaaaag tgtcgctgga gctgggcggc aacgcgccgt
     3361 ttatcgtctt tgacgatgcc gatctcgata aagccgtgga aggcgcgctg gcctcgaaat
     3421 tccgcaacgc cgggcaaacc tgcgtctgcg ccaaccgttt ggcgtgtatg accgctttgc
     3481 cgaaaaattg cagcaggcgg tgagcaaact gcacatcggc gacgggctgg ataaaggcgt
     3541 caccatcggg ccgctgatcg atgaaaaagc ggtagcaaaa gtggaagagc atattgccga
     3601 tgcgctggag aaaggtgcgc gtgtggtttg cggcgctaaa gcggatgaac gcggcggcaa
     3661 cttcttccag ccgaccattc tggtggacgt gccggccaac gccaaagtgt cgaaagaaga
     3721 gacgttcggc cccctcgccc cgctgttccg ctttaaagat gaagccgatg tgattgcgca
     3781 agccaatgac accgagtttg gccttgccgc ctatttctac gcccgtgatt taagccgcgt
     3841 cttccgcgtg ggcgaagcgc tggagtacgg catcgtcggc atcaataccg gcattatttc
     3901 caatgaagtg gccccgttcg gcggcatcaa agcctcgggt ctgggtcgtg aaggttcgaa
     3961 gtatggcatc gaagattact tagaaatcaa atatatgtgc atcggtcttt aactggagaa
     4021 tgcgaatgaa cagcaataaa gagttaatgc agcgccgcag tcaggcgatt ccccgtggcg
     4081 ttgggcaaat tcacccgatt ttcgctgacc gcgcggaaaa ctgccgggtg tgggacgttg
     4141 aaggccgtga gtatcttgat ttcgcgggcg ggattgcggt gctcaatacc gggcacctgc
     4201 atccgaaggt ggtggccgcg gtggaagcgc agttgaaaaa actgtcgcac acctgcttcc
     4261 aagtgctggc ttacgagccg tatctggagc tgtgcgagat tatgaatcag aaggtgccgg
     4321 gcgatttcgc caagaaaacg ctgctggtta cgaccggttc cgaagcggtg gaaaacgcgg
     4381 taaaaatcgc ccgcgccgcc accaaacgta gcggcaccat cgcttttagc ggcgcgtatc
     4441 acgggcgcac gcattacacg ctggcgctga ccggcaaggt gaatccgtac tctgcgggca
     4501 tggggctgat gccgggtcat gtttatcgcg cgctttatcc ttgcccgctg cacggcataa
     4561 gcgaggatga cgctatcgcc agcatccacc ggatcttcaa aaatgatgcc gcgccggaag
     4621 atatcgccgc catcgtgatt gagccggttc agggcgaagg cggtttctac gccgcgtcgc
     4681 cagcctttat gcagcgttta cgcgctctgt gtgacgagca cgggatcatg ctgattgccg
     4741 atgaagtgca gagcggcgcg gggcgtaccg gcacgctgtt tgcggtggag cagatgggcg
     4801 tggcgccgga tcttaccacc tttgcgaaat cgatcgctgg cgggcgtcac cgggcgcgcg
     4861 gaagtgatgg acgctgttgc accgggcggt ctgggcggca cctatgccgg taacccgatt
     4921 gcctgcgtgg ctgcgctgga agtgttgaag gtgttcgagc aggaaaatct gctgcaaaaa
     4981 gccaacgatc tggggcagaa gctaaaagac ggattgttgg cgatagccga aaaacacccg
     5041 gagatcggcg acgtacgcgg gctgggagcg atgattgcca tcgagctgtt tgaagatggc
     5101 gatcacagca aaccggacgc aaaactcacc gccgggatcg tggcacgcgc ccgcgataag
     5161 ggcctgattc ttctctcctg cggcccgtat tacaacgtgc tgcgcatcct tgtactgctc
     5221 accattgaag acgctcagat ccgtcagggt ctggagatta tcggtcagtg ttttgatgag
     5281 gcgaagcagt agcaccgtcc ccatgcagga ggcgacgctg cgcgtcttgt ccggcctacg
     5341 agaatccagg tcggataagg cgtttacgcc gcatccgaca atctgtacgt gaacaggaat
     5401 aaatctatgt cggccggata atgcggagcc gctctccggc aaaaagaatc aataacaatt
     5461 aaacgcgtga cccggcgcgg gaaatgtcgg ggcgctctcc caagtgacac actttcgaga
     5521 ggattcagga tggggcaatc atcgcaacca catgagttag gcggcgggct gaaatcacgc
     5581 cacgtcacca tgttgtctat tgccggtgtt atcggcgcaa gtctgtttgt cggttccagc
     5641 gtcgccatcg ccgaagcggg cccggcagta ttactggcct atctgttcgc cgggctactg
     5701 gtggttatga ttatgcggat gttggcggaa atggcggttg ccacgcccga taccggttcg
     5761 ttttccacct atgccgataa agccattggt cgctgggcgg gctataccat cggctggttg
     5821 tactggtggt tttgggtact ggttatcccg ctggaagcca acatcgccgc catgatcctg
     5881 cactcgtggg ttccaggcat tcccatctgg ttattttccc tcgtcattac ccttgcctta
     5941 actggcagta atttattaag cgttaaaaac tacggcgaat ttgagttctg gctggcgctg
     6001 tgcaaagtca tcgctatcct ggcctttatt ttccttggtg cagtcgcaat tagcggtttt
     6061 tacccttatg ccgaagtgag cgggatctca agattgtggg atagcggcgg ctttatgccc
     6121 aacggcttcg gtgcggtatt aagcgcgatg ttgatcacca tgttctcgtt tatgggcgca
     6181 gaaattgtca ccattgccgc cgcggaatcc gacacgccgg aaaaacatat tgtccgcgcc
     6241 actaactcgg ttatctggcg tatttctatc ttctatttgt gctctatttt tgtcgtcgtg
     6301 gcattaattc cgtggaatat gcccggacta aaagccgtcg gttcttatcg ctcggtactg
     6361 gaattgctca atattcccca tgcgaaatta atcatggact gtgtgatatt actttccgta
     6421 accagctgcc tgaactcggc gctgtatacc gcttccagga tgctctactc cttaagccgt
     6481 cgcggagatg cgcctgcggt aatgggtaaa atcaaccgca gtaaaacccc gtacgtggcg
     6541 gtgttactct ccaccggcgc ggcattctta acggtggtgg tgaactatta cgtgcctgtg
     6601 aaggtattta aatttctgat cgacagctcc ggcgctatcg ccctgctggt ttatttagtc
     6661 atcgccgttt cacagttgcg gatgcgcaaa attctgcgag cagaaggaag cgaaattcgc
     6721 ttgcgcatgt ggctttatcc ctggctcacc tggctggtca tcggctttat tacctttgtg
     6781 ttggtagtga tgctattccg cccggcgcaa cagttagaag tgatctccac cgacttatta
     6841 gcaataggga ttatctgtac cgtgccaatt atggcggctg gaataagctg ataatgtggc
     6901 aaaaaacacc cgttcataat acgcgctgat aataatcagg agtcacacca tgaccattac
     6961 gtctctggat ggctatcgct ggctgaagaa cgatattatt cgcggtaatt ttcaaccgga
     7021 tgaaaaatta cggatgagtt tgctgacatc gcgttatgca cttggcgttg ggccgttgcg
     7081 ggaagcactt tctcaactgg tggcggaacg gctggtcacg gtggtgaatc aaaaagggta
     7141 tcgggtggcg tctatgtcag agcaggagct gctcgatatt ttcgatgccc gcgccaatat
     7201 ggaagcgatg ttagtgagtc tggcgattgc ccgcggtagc gatgagtggg aggcagacgt
     7261 tctcgcaaaa gcgcatctgc tcagtaagct ggaagcctgt gacgccagcg agaaaatgct
     7321 tgatgagtgg gatctgcgtc atcaggcgtt tcatacagca attgtggcgg gctgtggttc
     7381 tcactatttg ctgcaaatgc gtgaacggtt gtttgatctg gcggcgcgct atcgatttat
     7441 ctggctgcgg cgaacggtgc tttcggtaga aatgctggag gataaacacg atcagcacca
     7501 gaccctgact gcggcggtac tggcgcgaga taccgcgcgc gccagtgagt taatgcgcca
     7561 gcatttactg acgccaattc ccattatcca gcaggcgatg gctggcaatt aactactctt
     7621 ccggaatacg caacacttgc cccggataaa ttttatccgg gctttttagc atcggtttat
     7681 tcgcttcgaa gattttattg tacagattag cgttaccgta gacctgtttg gaaatggcac
     7741 tcagagtgtc gccagactta acggtataaa actggctggc agtggctggt gtcgccgttt
     7801 tcacctgatc atcaacactg gcaataccgg aaatattccc caccgcaaca aggattttct
     7861 ccttcgcctc ctgactcagg ccgtcaccag tgaccgtcgc tttgccgtcg gcaatttgaa
     7921 tgttcacttt atcggcatca ggtataccgg ttttgctcag atgctcctgc accttcttcg
     7981 cctgatcgtc tttatcgtgc tgacctgtaa ccgcgtccca gagtttttct ccggcatctt
     8041 tcacaaaatt gaacagaccc atagctacct cattcgttaa cggaaacact cagaaagtgt
     8101 agcagaggaa cgcggcgcgt taacgccgcg ggggggagca caggtttaat cgcgggtttg
     8161 cacccagaac gcgtgaatca aaccggggat atacccaagc agtgtcagca gaatattaat
     8221 aatgaacgcc caaccgaacc ctttaccgag cagcacgccg agcggcggca gaatgatggt
     8281 gatgacgatt ctccagaaac ccatatgtac tccctataag aaaattactc attgtttaaa
     8341 aagagatttt atctcttaag cgtagtaatt ttagcggagc ctgccagttt cagcttacgc
     8401 ttttactctc atttacgctg ctgaggctgg tatatgttgc atttttagtt tagagtttac
     8461 ttaatttaga aaatacttaa ataaatatga ctgaactcgc gcaattacag gccagtgccg
     8521 aacaggcagc ggccttattg aaagcaatga gccatcctaa acgattgctg attctgtgca
     8581 tgcttagcgg ttcccccggc accagcgcgg gagagctgac gcgcattacc ggactgagtg
     8641 cctctgcgac atcacagcat ctcgctcgta tgcgggacga agggcttatc gacagccaac
     8701 gggatgccca acgcattcta tattccatta aaaatgaggc ggtaaatgcc attatcgcca
     8761 ccctgaaaaa tgtctattgt ccgtaaggag tcactatggc tttgacaacc atttcgccgc
     8821 atgatgcaca agaattaatc gcacgcggcg caaagttaat cgatattcgt gacgctgatg
     8881 aatatcttcg tgaacatatt cctgaagcag atctggctcc attatccgtg ctggaacagt
     8941 caggtcttcc acctaaatta cgtcgcgagc aaattatatt ccactgccag gcaggtaaac
     9001 gcaccagtaa taacgccgat aaattagcag cgattgccgc ccccgcagaa atatttttac
     9061 tcgaagatag gattgatggc tggaaaagag ctggattgcc agtagcggta aataaatctc
     9121 aacccttgcc gttaatgcgc caggtgcaga tcgctgcggg tggtttaata ttaatcggcg
     9181 ttgtactggg ttataccgta aatagcggtt tcttcttatt aagtggcttt gttggtgccg
     9241 ggttactgtt tgcaggaatc agcggttttt gtggaatggc aaggttgtta gataagatgc
     9301 cgtggaacca acgagcttga gaagcgacgc cggacgcgcc ctagcagcga catccggcct
     9361 cagtaattag atcaggaaat cgtcgagaga tttaccttct gccagcgcct gagcaattgg
     9421 cttcggtgta cggccctgac cggtccaggt cttagtttca ccgttaacat cagtgaattt
     9481 atatttcgcc ggacgcggct ggcgtttttt accagcacgt ggtgcagcag cagagctatt
     9541 acccaataac tcttccgggt taattccgtc agctttcatc agctccagcc aggtgctaat
     9601 tttttcctga cgctcagcca gttcacgctg ctgctgttct tcttcttcac gtctttcttt
     9661 agtgacaacc ctgaattttt cgagcatttc ttcaagaacg tcaatggaga attcgcgagc
     9721 catcgcacgg agggttcgaa tgttatttaa actttgtaac attacggaca taataaagaa
     9781 aaccttttaa cgccaaaaca aaaaagtatt tcgtcgcgta gtgtaagcga tcacttatta
     9841 tatttccaca aaatttaatc tatttacagt cctgcgcgag attatttcag ctgttatttt
     9901 ctgaaactct cagaataatt tcaagattat atattagcca ttttgatgag taatttaggt
     9961 tattggtgaa cagagtggcg ctctctgctc gctaactccg caagcctgat aaaaccgaag
    10021 tgaataaaaa tgcaatataa cagaataaaa gaaaacaccc ctcactggtg ctttctgctg
    10081 ttaatgtaca ttttctggca tcaaaaatgc tgtaaatgac ccggtcacga caaacaccga
    10141 attctctacg cttatagcgt tacctcaccc ccaaactttc attagtgaaa ttaaaggcaa
    10201 ggtcatatcc aattgattta attgaaaata aatcatatcg gcatgtaaaa atccacaatg
    10261 tacaaaaaac gagctgttac ggaatatttt atctacaaaa aactgactaa ataaaagttt
    10321 ttcactaatt gattagtcat agccagcgat atacgctatg cgaaaatgca gatggcaatg
    10381 agatccactg ctttcatctc cattaacatc ccattacgct ttattaagga gcattagcat
    10441 gttctcaccg cagtcacgct tgcgtcatgc agttgcagat acgttcgcga tggttgttta
    10501 ctgttctgtc gtgaacatgt gtattgaagt tttcctctcc ggaatgagct tcgaacagtc
    10561 tttttattcc agattggtag cgattccggt gaacatctta attgcatggc catacggtat
    10621 gtaccgcgat ctgtttatgc gcgcggcacg caaagttagc ccgtcgggct ggataaaaaa
    10681 tctggctgat atcctggctt atgtgacgtt ccagtcaccg gtgtatgtgg cgatcttgtt
    10741 agtggtgggc gcagactggc atcagattat ggcggcggtc agttcaaaca tcgttgtttc
    10801 gatgttgatg ggggcggttt atggctactt cctcgattat tgccgccgac tgtttaaagt
    10861 cagccgttac cagcaggtaa aagcctgact cttcttttcg cgactggcat cgccagtcgc
    10921 gtctttaact ggcttcgcca aataaaccat tcaaataacg ttcaagcgca acacgcgaac
    10981 tgaagccatg cgggataccg taatgctcgt gtcgctcgcc tgctaaatag agcggaaatt
    11041 gctggtaatg atcgcaagtt ttaatatcgg aagcgggttc agccagtgtt gaactgcgtt
    11101 cttttaatgt tggatgaatt accagagcgg tacgtcccat tcgcgcttcg cgattaacat
    11161 agacataatt ttccccacgg cggtaaccat acgctttctg ggttaccaca tcgacagtaa
    11221 aaccgacttt ttcaagtacg cgcgccacct cgtctggtcg taaatacata tttgatcctc
    11281 gttattttca accgcg
//
LOCUS       DAJTMC010000134        11259 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.134, whole genome shotgun sequence.
ACCESSION   DAJTMC010000134 DAJTMC010000000
VERSION     DAJTMC010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11259
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.134"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            1..699
                     /gene="hdhA"
                     /locus_tag="OO130_003159"
                     /pseudo
     CDS             1..699
                     /gene="hdhA"
                     /locus_tag="OO130_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
     gene            928..1518
                     /gene="uidR"
                     /locus_tag="OO130_003160"
     CDS             928..1518
                     /gene="uidR"
                     /locus_tag="OO130_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HCR5366463.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTSMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            1906..3717
                     /gene="uidA"
                     /locus_tag="OO130_003161"
     CDS             1906..3717
                     /gene="uidA"
                     /locus_tag="OO130_003161"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HCR5366464.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMVYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KDEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLEWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            3714..5087
                     /gene="uidB"
                     /locus_tag="OO130_003162"
     CDS             3714..5087
                     /gene="uidB"
                     /locus_tag="OO130_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HCR5366465.1"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGALFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHSSKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYERYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARSGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQVQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKVQQQ
                     LISDITN"
     gene            5126..6391
                     /gene="uidC"
                     /locus_tag="OO130_003163"
     CDS             5126..6391
                     /gene="uidC"
                     /locus_tag="OO130_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HCR5366466.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQRGLFDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPCENSLLKLGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRLTPQFTLTFAPIVTRGYESSKRDVRIEGTGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(6617..8125)
                     /locus_tag="OO130_003164"
     CDS             complement(6617..8125)
                     /locus_tag="OO130_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HCR5366467.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITVAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATEFMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYTNGQITLNGQKMSLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(8226..9401)
                     /gene="manA"
                     /locus_tag="OO130_003165"
     CDS             complement(8226..9401)
                     /gene="manA"
                     /locus_tag="OO130_003165"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416130.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HCR5366468.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     RNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPNAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            9600..11246
                     /gene="fumB"
                     /locus_tag="OO130_003166"
     CDS             9600..11246
                     /gene="fumB"
                     /locus_tag="OO130_003166"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCR5366469.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGCDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
BASE COUNT         2809 a         2633 c         2906 g         2911 t
ORIGIN      
        1 tggtaaagaa atcgccatta cattcgcgac agctggcgca tctgtggtgg tcagtgatat
       61 taacgccgac gcagctaacc atgttgtaga cgaaattcaa caactggggg ggcaggcatt
      121 tgcctgccgt tgtgatatta cttccgaaca ggaactctct gcactggcag atttgctatc
      181 agtaagctgg gtaaagttga tattctggtt aacaacgccg gtggcggtgg acctaaaccg
      241 tttgatatgc caatggcgga ttttcgctgt gcttatgaac tgaatgtgtt ttcttttttc
      301 catctgtcac aacttgttgc gccagaaatg gaaaaaaatg gcggtggcgt tattctgacc
      361 atcacttcta tggcggcaga aaataaaaat ataaacatga cttcctatgc atcatctaaa
      421 gctgcggcca gtcatctggt cagaaatatg gcatttgacc tgggtgaaaa aaatattcgg
      481 gtgaatggca ttgcgccggg ggcaatatta accgatgccc tgaaatccgt tattacacca
      541 gaaattgaac aaaaaatgtt acagcacacg ccgatcagac gtctgggtca accgcaagat
      601 attgctaacg cggcgctgtt cctttgctcg cctgcagcca gctgggtaag cggacaaatt
      661 ctcaccgtct ccggtggtgg ggtacaggag ctcaattaat acactaacga accggtaaac
      721 agccgtatgt gttgtttacc gggatagcgt gtgcaattca tcaacgtctc tgctaaacaa
      781 ctggcagcca aatcacggct attggttaac caatagctgg atgaaaagca tacgaataga
      841 gtgtgccttc gcactattca acagcaatga taggcgctca cctgacaacg cggtaaacta
      901 gttattcacg ctaactataa tggtttaatg atggataaca tgcagactga agcacaaccg
      961 acacggaccc ggatcctcaa tgctgccaga gagatttttt cagaaaatgg atttcacagt
     1021 gcctcgatga aagccatctg taaatcctgc gccattagtc ccgggacgct ttatcaccat
     1081 ttcatctcca aagaagcctt gattcaggcg attatcttac aggaccagga gagggcgctg
     1141 gcccgtttcc gggaaccgat tgaagggatt cattttgttg actatatggt cgagtctatt
     1201 gtctctctca cccatgaagc ctttgggcaa cgggcgctgg tggtcgaaat tatggcggaa
     1261 gggatgcgta acccacaggt cgccgccatg cttaaaaata agcatatgac gatcactgaa
     1321 tttgttgccc agcggatgcg tgatgcccag caaaaaggcg agataagccc agacatcaac
     1381 acgtcaatga cttcacgttt actgctggat ctgacctacg gtgtactggc cgatatcgaa
     1441 gcggaagatc tggcgcgtga agcgtcgttt gctcagggat tacgcgcgat gattggcggt
     1501 atcttaaccg catcctgatc ctctcatttc gcgggctggt gataactgtg cccgcgtttc
     1561 atactttaat ttccctgtac aaaatacaat ctagccggga tcggagaatt cccccccaaa
     1621 atattcaccg tagccatatg tcatgagagt ttatcgttct caagatgccc gaacgaacgt
     1681 tcgattgctt attttgtggc ttctgtcaac gctgttttaa agattaatgc gatctatatc
     1741 acgctgtgcg tattgcagat tttggttttt tgatcgcggt gtcagttctt tttatttcca
     1801 tttctcttcc atgggtttct cacagataac tgtgtgcaac acagaattgg ttaactaatc
     1861 agattaaagg ttgaccagta tttttatttg aatgaggagt cccttatgtt acgtcctgta
     1921 gaaaccccaa cccgtgaaat caaaaaactc gacggcctgt gggcattcag tctggatcgc
     1981 gaaaactgtg gaattgatca gcgttggtgg gaaagcgcgt tacaagaaag ccgggcaatt
     2041 gctgtgccag gcagttttaa cgatcagttc gccgatgcag atattcgtaa ttatgtgggc
     2101 aacgtctggt atcagcgcga agtctttata ccgaaaggtt gggcaggcca gcgtatcgtg
     2161 ctgcgtttcg atgcggtcac tcattacggc aaagtgtggg tcaataatca ggaagtgatg
     2221 gagcatcagg gcggctatac gccatttgaa gccgatgtca cgccgtatgt tattgccggg
     2281 aaaagtgtac gtatcaccgt ttgtgtgaac aacgaactga actggcagac tatcccgccg
     2341 ggaatggtga ttaccgatga aaacggcaag aaaaagcagt cttacttcca tgatttcttt
     2401 aactatgccg ggatccatcg cagcgtaatg gtctacacca cgccgaacac ctgggtggac
     2461 gatatcaccg tggtgacgca tgttgcgcaa gactgtaacc acgcgtctgt tgactggcag
     2521 gtggtagcaa atggtgatgt cagcgttgaa ctgcgtgatg cggatcagca ggtggttgca
     2581 actggacaag gtaccagcgg gactttgcaa gtggtgaatc cccacctctg gcaaccgggt
     2641 gaaggttatc tctatgaact gtgcgtcaca gccaaaagcc agacagagtg tgatatctac
     2701 ccgctgcgcg tcggcatccg gtcagtggca gtgaaggacg aacagttcct gattaaccac
     2761 aaaccgttct actttactgg ctttggtcgt catgaagatg cggatttgcg aggcaaagga
     2821 ttcgataacg tgctgatggt gcacgatcac gcattaatgg actggattgg ggccaactcc
     2881 taccgaacct cgcattaccc ttacgctgaa gagatgctcg agtgggcaga tgaacatggc
     2941 atcgtggtga ttgatgaaac tgcagctgtc ggctttaacc tctctttagg cattggtttc
     3001 gaagcgggca acaagccgaa agaactgtac agcgaagagg ctgtcaacgg ggaaactcag
     3061 caggcgcact tacaggcgat taaagagctg atagcgcgtg acaaaaacca cccaagcgtg
     3121 gtgatgtgga gtattgccaa cgaaccggat acccgtccgc aaggtgcacg agaatatttc
     3181 gcgccactgg cggaagcaac gcgtaaactc gacccgacgc gtccgatcac ctgcgtcaat
     3241 gtaatgttct gcgacgctca caccgatacc atcagcgatc tctttgatgt gctgtgcctg
     3301 aaccgttatt acggatggta tgtccaaagc ggcgatttgg aaacggcaga gaaggtactg
     3361 gaaaaagaac ttctagcctg gcaggagaaa ctgcatcagc cgattatcat caccgaatac
     3421 ggcgtggata cgttagccgg gctgcactca atgtacaccg acatgtggag tgaagagtat
     3481 cagtgtgcat ggctggatat gtatcaccgc gtctttgatc gcgtcagcgc cgtcgtcggt
     3541 gaacaggtat ggaatttcgc cgattttgcg acctcgcaag gcatattgcg cgttggcggt
     3601 aacaagaagg ggatcttcac ccgcgaccgc aaaccgaagt cggcggcttt tctgctgcaa
     3661 aaacgctgga ctggcatgaa cttcggtgaa aaaccgcagc agggaggcaa acaatgaatc
     3721 aacaactctc ctggcgcacc atcgtcggct acagcctcgg tgacgtcgcc aataacttcg
     3781 ccttcgcaat gggggcgctc ttcctgttga gttactacac cgacgtcgct ggcgtcggtg
     3841 ccgctgcggc gggcaccatg ctgttattgg tgcgggtatt cgatgccttc gccgacgtct
     3901 ttgccggacg agtggtggac agtgtgaata cccgctgggg aaaattccgc ccgtttttac
     3961 tcttcggtac tgcgccgtta atgatcttca gcgtgctggt attctgggta ccgaccgact
     4021 ggagccatag cagcaaagta gtgtatgcat atttgaccta catgggcctc gggctttgct
     4081 atagcctggt gaatattcct tatggttcac ttgctaccgc gatgacccaa caaccacaat
     4141 cccgcgcccg tctgggcgcg gctcgtggga ttgccgcttc attgaccttt gtctgcctgg
     4201 catttctgat agggccgagc attaagaact ccagcccgga agagatggtg tcggtatacc
     4261 atttctggac gattgtgctg gcgattgccg gaatggtgct ttacttcatc tgcttcaaat
     4321 cgacgcgtga gaatgtggta cgtatcgttg cgcagccgtc attgaagatc agtctgcaaa
     4381 ccctgaaacg gaatcgcccg ctgtttatgt tgtgcatcgg tgcgctgtgt gtgctgattt
     4441 cgacctttgc ggtcagcgcc tcgtcgttgt tctacgagcg ctatgtgtta aatgataccg
     4501 ggctgttcac tgtgctggta ctggtgcaaa acttggttgg tactgtggca tcggcaccgc
     4561 tggtgccggg gatggtcgcg aggagcggta aaaagaatac cttcctgatt ggcgctttgc
     4621 tgggaacctg cggttatctg ctattcttct gggtttccgt ctggtcactg ccggtggcgt
     4681 tggttgcgtt ggccatcgct tcaattggtc agggcgttac tatgactgtg atgtgggcac
     4741 tggaagctga taccgtagaa tacggtgaat acctgaccgg cgtgcgaatt gaagggctca
     4801 cctattcact attctcattt acccgtaaat gcggtcaggc aatcggaggt tcaattcctg
     4861 cctttatttt gggattaagc ggatatatcg ccaatcaggt gcaaacgccg gaagttatta
     4921 tgggcatccg cacatcaatt gccttagtac cttgcggatt tatgctactg gcattcgtta
     4981 ttatctggtt ttatccgctc acggataaaa aattcaaaga aatcgtggtt gaaattgata
     5041 atcgtaaaaa agtgcagcag caattaatca gcgatatcac taattaatat tcaataaaaa
     5101 taatcagaac atcaaaggtg caactatgag aaaaatagtg gccatggccg ttatttgcct
     5161 gacggctgcc tctggcctta cctctgctta tgcggcgcaa ctggctgacg atgaagcggg
     5221 actacgcatc agactgaaaa acgaattgcg cagggcggat aagcccagtg ctggcgcggg
     5281 aagagatatt tacgcatggg tacagagagg attgttcgat ttcaatagtg gttattattc
     5341 caatattatt ggcgttgaag gcggggcata ttatgtttat aaattaggtg ctcgtgctga
     5401 tatgagtacc cggtggtatc ttgatggtga taaaagtttt ggctttgccc tgggggcagt
     5461 aaaaataaaa ccctgtgaaa atagcctgct taaattaggt cgcttcggga cggattatag
     5521 ttatggtagc ttaccttatc gtattccgtt aatggctggc agttcgcaac gtacattacc
     5581 gacagtttct gaaggagcat taggttattg ggctttaaca ccaaatattg atctgtgggg
     5641 aatgtggcgt tcacgagtat ttttatggac tgattcaaca accggtattc gtgatgaagg
     5701 ggtgtataac agccagacgg gaaaatacga taaacatcgc gcacgttctt ttttagccgc
     5761 cagttggcat gatgatacca gtcgctattc tctgggggca tcggtacaga aagatgtttc
     5821 caatcagata caaagtattc tcgagaaaag cataccgctc gacccgaatt atacgttgaa
     5881 aggggaattg ctcggctttt acgcgcagct cgaaggttta agtcgtaata ccagccagcc
     5941 caatgaaacg gcgttggtta gtggacaatt gacctggaat gcgccgtggg gaagcgtatt
     6001 tggcagtggt ggttatttgc ggcatgcaat gaatggtgcc gtggtggata ccgacattgg
     6061 ctatcccttt tcattaagtc ttgatcgtaa ccgtgaagga atgcagtcct ggcaattggg
     6121 cgtcaactat cgtttaacgc cgcaatttac actgacattt gcaccgattg tgactcgcgg
     6181 ctatgaatcc agtaaacgag atgtgcggat tgaaggcacg ggtatcttag gtggtatgaa
     6241 ctatcgggtc agcgaagggc cgttacaagg gatgaatttc tttcttgctg ccgataaagg
     6301 gcgggaaaag cgcgatggca gtacgctggg cgatcgcctg aattactggg atgtgaaaat
     6361 gagtattcag tatgacttta tgctgaagta agaaataacg ccggagagaa aaacctccgg
     6421 cgtttcagat tgttgacaaa gtgcgcgttt tttatgccgg atgcggcgta aacgccttat
     6481 ccagcctaca aaaactcata aattcaaagt attgcaggaa aaggtaggcc tgataagcgt
     6541 agcgcatcag gcaatctctg gtttgttttc agatgaaaac gccgaagtga aaattctccg
     6601 gcgttttggc cgtgaattac tgctgcggaa ttgccggtac agccggaacg ttaagagctg
     6661 gcatcgcaaa catgccaaca aaatcttcta acgacatttt ctgcccattt aacgttatct
     6721 gaccgttagt atattgcagg ctggtggtga tggtattgtc ctgcaaggtg gtcagacgga
     6781 acatctgccc cattgctgat gcaccttcaa cttgctgttt cgccagtttt ttcgcttgat
     6841 cttcctgata accttccagc ttcgctacct gagtcataaa ctcagttgcc atatccaccg
     6901 gaatggtcag tttcgcatcc agagatttaa ccgaacgatc tacttcctgc gccagcgttt
     6961 gcggcgcttc tttagtcgtt gccggatctt tcaggaacag cgacagattc agcgcacttt
     7021 caccctgact gtttttccag cttagcggcg cgacagtaat caccggatcg cctttcaaca
     7081 tcagcggcag ggcgctaaag aaggcttccg tcactttctc ctgataaagt tcgggattgt
     7141 tggcaatttc tggctgtgcc agcagcgcct gagtttgcgc gttatattgc tggctgaact
     7201 gatgccaggc ttcaccatca atctggccga cttttaaagt cagcttgccg ctgcccagat
     7261 cctgattctg taccttcagg ctgtttagcg agtaatccag ttggctattg atcgttttac
     7321 cgtcattgac cagatccgat ttaccgctga tctccatgcc ttccagcagt gccagttctt
     7381 tgccttccac tgaaatggtc attttttcca gtgacagttt ttggtttcct acgcgctcac
     7441 caaaacttgc cagcgtgctg gaaccgtcgg ttttcagatt attaaaggtc aactgcactt
     7501 tctggttgta ttcgttaact gcgtctatcc gaccactttg cgcctccccg gaaagggaga
     7561 tggctttgcc gtctctgtca gcatttaact ggaactcgcc gccgctaaag gcgacttttt
     7621 catccttttg ctcgtaattc agtggcttga gcgaaatatc ggaactggaa tcaccgctgt
     7681 aaccaatgcg cgagttaatc tcaaaaggcg tttcaccttt tgccatatca aacagtggtt
     7741 tgcttacttc gttattaacc agcgtggttt gaattgatgc catcgacggg atcaggttca
     7801 gttttttaag ctgggcaagc gggaagggac catgatcaac cgattcgttg aagatgacgc
     7861 tctgaccgct tttaatccac ggattttctt tcccggcaat gggtttcacc aacagttgca
     7921 actggctgct gaatacgccg cgatgatagt tttgataact cacttccagg ttggattcag
     7981 gagctgtcag ttttagttgc gcgttcgcct gcgcgaccat gtcttcgaga tgggtttcaa
     8041 tcttcttgcc tgtataccat gcgccgcctg tccagactac gcctagcgca acaatgacgc
     8101 ctaccgctac cagcgattta ttcataatga ttatccataa aatgaaatca ggcggactgg
     8161 ccgcctgaag gtgttataag cctttaataa gcttagcaag agatgttaat tttttcagta
     8221 agctcttaca gcttgttgta aacacgcgct aaacggccgt ggcctttgac agtcaccggt
     8281 gattcgttgg cggcaataaa cgctgattca cccggtttaa gctgtaactg ctgagaacct
     8341 ttccacaacg ttgcatcgcc ttcgacgcag aacaaaatgg cggcactctg ctggctaatg
     8401 gtggtttctt tatcactaag gtcatgcagc gagaaggcaa aatcatccac tggaatcggg
     8461 aagtccagtt ctgcaccttg tttcaccggc tgggtcaaca actggttagc cggtttggct
     8521 tcgaatttca cattggcaac cagttccgga atatcaatgt acttaggcgt cagacccgca
     8581 cgcagcacgt tatcggagtt cgccatcact tccagcgcca cgccttgcag gtaagcgtgc
     8641 ggtgtttcag cgaacaggaa catcgcttcg ccaggattca atttcaccac attcagtaac
     8701 agcggggaga acaggccgct gtcttccggg taaaattcag aaattaaacg aatcgtttgc
     8761 cacggttcac cctgctgact gtcgagcgcc gattttaaaa tcgccagcgc gcgggatttt
     8821 tcttcaccct gcatattcaa caggctggcg aacagttcgc ttaaacgttc ggcattaggc
     8881 tgttgtaaaa agtgagcaat tgccggatgt gcacctgcga ctggctggag tagggagaca
     8941 atctcggaaa actcacgaaa cgcgttcatc gcaaggaaag gcgtcagcgc aaaaaccagc
     9001 tccggcttgt ggttaggatc tttatagtta cgctcggcgg catccatcgg gatacctgcg
     9061 gcattttctt tggcaaaacc gatttcagaa ttgcgtttgt ttggatgaac ctgaatggag
     9121 agcggctgtg ctgcgcataa tactttgaac aggaaaggca gttcgccaaa gcgtttggca
     9181 acggcctctc cgagcagagt cgatttatca ctctcaatca catcacgcag tgaaacgata
     9241 tctccggcgg cattctgcac tcgtgaactg cttttcggat gtgcgcccat ccacagctcg
     9301 gccatcggct ggctggacgg attttccata ccataaagtt cagtcaacgc cgttttgctg
     9361 ccccaggcat agttttgcac tgagttaatg agtttttgca tgatcaatcc ctgttttaat
     9421 gtggaaatta atcccactat taaagcaaga atcctacggg aagtaacctg gagccgcaaa
     9481 aagtcgtact agtctcagtt tttgttaaaa aagtgtgtag gatattgtta ctcgctttta
     9541 acagggcaac ggaacacccg cccagagcat aaccaaacca ggcagtaagt gagagaacaa
     9601 tgtcaaacaa accctttcat tatcaggctc cttttccact caaaaaagat gatactgagt
     9661 attacctgct aaccagcgaa cacgttagcg tatctgaatt tgaagggcag gagattttga
     9721 aagtcgcacc cgaagcgtta actctgttgg cgcgtcaggc gtttcatgat gcgtcattta
     9781 tgctgcgtcc ggctcaccaa caacaggtgg ccgacattct gcgtgacccg gaagccagcg
     9841 aaaatgataa atatgtggcg ctgcaattcc tgcgcaactc cgacatcgcg gcgaaaggtg
     9901 ttctgccaac ctgtcaggat accggcactg cgattattgt tggtaaaaaa gggcagcgtg
     9961 tatggaccgg tggctgtgat gaagcggcgc tggcgcgcgg tgtctataac acttatatcg
    10021 aagataattt gcgctactcg caaaacgcgc cgctggatat gtataaagag gtgaataccg
    10081 gcaccaatct gccagcgcag atcgatcttt atgccgttga tggcgacgag tacaaattcc
    10141 tctgtatcgc caaaggtggt ggttcggcaa acaagacgta tctctatcag gaaaccaaag
    10201 cgttactaac gccggggaaa ctgaaaaatt acctggttga gaagatgcgc acgctgggta
    10261 cggcggcctg tcctccgtat catattgcgt tcgtaattgg tggaacttct gcagaaacta
    10321 accttaaaac agtgaaactg gcttccgcta aatactatga cgaactgcca acggaaggga
    10381 atgagcacgg tcaggcattc cgcgatgtgg aactggaaaa agaattgctg atcgaagcgc
    10441 aaaatctcgg tctgggtgcg cagtttggtg gtaaatactt cgctcacgac atacgcgtga
    10501 ttcgcctgcc acgtcacggc gcatcctgtc cggttggtat gggtgtttcc tgctctgctg
    10561 accgtaatat caaagcgaag atcaaccgtc aggggatctg gatcgaaaaa ctggaacata
    10621 atccaggtaa atatatccct gaagagctgc gtaaagcggg agaaggcgaa gcggtgcgcg
    10681 ttgaccttaa ccgtccgatg aaagagatcc tcgcacagtt gtcgcagtat cccgtttcta
    10741 cacgcttatc gcttaacggc acgattatcg tcggtcgtga tattgctcac gccaaactga
    10801 aagagcggat ggataacggt gaagggctgc cgcagtacat taaagatcat ccgatttact
    10861 acgcgggtcc ggccaaaacg ccggaaggtt atgcctccgg ttctcttggc ccaacgaccg
    10921 ccggacggat ggattcttat gtcgatcaac tgcaagcgca gggcggaagt atgatcatgc
    10981 tggcgaaagg caaccgcagc cagcaggtga cggatgcctg taaaaaacac ggcggcttct
    11041 accttggcag tatcggtggt ccggccgctg tattggcgca gggaagtatt aagagcctgg
    11101 aatgtgttga atatccggaa ctgggaatgg aagccatctg gaaaattgaa gtggaagatt
    11161 tcccggcgtt tatccttgtg gatgataaag gaaatgactt cttccagcag atacaactca
    11221 cacagtgcac tcgctgtgtg aaataaacag agccgccct
//
LOCUS       DAJTMC010000135        11245 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.135, whole genome shotgun sequence.
ACCESSION   DAJTMC010000135 DAJTMC010000000
VERSION     DAJTMC010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11245
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.135"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(1..210)
                     /locus_tag="OO130_003167"
                     /pseudo
     CDS             complement(1..210)
                     /locus_tag="OO130_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            487..1362
                     /locus_tag="OO130_003168"
     CDS             487..1362
                     /locus_tag="OO130_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HCR5366470.1"
                     /translation="MRKFILISMITLASVSLGACRQNVTIKQDAPGQKAFLIDGQIYD
                     LHSGKIISSSELLADLATARHLIIGEKHDNAEHHQIELWLIQNLLIQRPQGSVLLEML
                     TSDQQPRVNQVKCWLKDNPVVRDSRVQELLNWQKGWSWEMYGDIVMQLLRGPYPLLNA
                     NIGREQMLALYKKNEFPKGKKSTAPVVQEALRETIIAMHEGNLESQQLTSMLSVQQQR
                     DRYMARQLLSAPVPSLLIAGGYHASKNMGVPLHMEDLATGTHPVVLMLAEKGMNITVD
                     HADYVWFVAPDTTKR"
     gene            1370..2146
                     /locus_tag="OO130_003169"
     CDS             1370..2146
                     /locus_tag="OO130_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000981087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HCR5366471.1"
                     /translation="MMNILHFPQSVKWSSWFICSLLLHGLIFLTFIWRFSEVQPAMSP
                     APAIMLQWAEIIEAPSSPLSLPVGIAQQESAVTEEKQQTEDRQQRPVTEDSDATIEIT
                     RKKKSSDGEKKKTRPPRKIKAQTSDSNPTAVSSNAAPQALVESSRIAAPFNSDSTKRD
                     NSEASWESRVKGHLNRYKRYPGDARKRARTGTAVVTFTVNTEGTIVSSFLEISSGTLS
                     LDREAIAVLERAQPLPKPPPEILEGGLFKVKMPITFKLKE"
     gene            2315..4576
                     /locus_tag="OO130_003170"
     CDS             2315..4576
                     /locus_tag="OO130_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HCR5366472.1"
                     /translation="MNVIKLAIGSGILLLSCGAYSQSISEKTNSDKKGAAEFSPLSVS
                     VGKTTSEQEALEKTGATSSRTTDKNLQSLDATVRSMPGTYTQIDPGQGAISVNIRGMS
                     GFGRVNTMVDGITQSFYGTSTSGTTAHGSTNNMAGVLIDPNLLVAVDVTRGDSSGSEG
                     INALAGSANMRTIGVDDVIFNGNTYGLRSRFSVGSNGLGRSGMIALGGKSDAFTDTGS
                     IGVMAAVSGSSVYSNFSNGSGINSKEFGYDKYMKQNPKSQLYKMDIRPDEFNSFELSA
                     RTYENKFTRRDITSDDYYIKYHYTPFSELIDFNVTASTSRGNQKYRDGSLYTFYKTSA
                     QNRSDALDINNTSRFTVADNELEFMLGSKLMRTRYDRTIHSAAGDPKANQESIENNPF
                     APSGQQDISALYTGLKVTRGIWEADFNLNYTRNRITGYKPACDSRVICVPQGSYDIDD
                     KEGGFNPSVQLSAQVTPWLQPFIGYSKSMRAPNIQEMFFSNSGGASMNPFLKPERAET
                     WQAGFNIDTRDLLVEQDALRFKALAYRSRIQNYIYSESYLVCSGGRKCSMAEVIGNDW
                     EGISDEYSDNMYIYVNSASDVIAKGFELEMDYDAGFAFGRLSFSQQQTDQPTSIASTY
                     FGAGDMTELPRKYMTLDTGVRFFDNALTLGTIIKYTGKARRLSPDFEQDEHTGAIIKQ
                     DLPQIPTIIDLYGTYEYNRNLTLKLSVQNLMNRDYSEALNKLNMMPGLGDESHPANSA
                     RGRTWIFGGDIRF"
     gene            4645..5820
                     /gene="senB"
                     /locus_tag="OO130_003171"
     CDS             4645..5820
                     /gene="senB"
                     /locus_tag="OO130_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterotoxin production-related protein TieB"
                     /protein_id="HCR5366473.1"
                     /translation="MNIFTLSKAPLYLLISLFLPTMAMAIDPPERELSRFALKTNYLQ
                     SPDEGVYELAFDNASKKVFAAVTDRVNREANKGYLYSFNSDSLKVENKYTMPYRAFSL
                     AINQDKHQLYIGHTQSASLRISMFDTPTGKLVRTSDRLSFKAANAADSRFEHLRHMVY
                     SQDSDTLFVSYSNMLKTAEGMKPLHKLLMLDGTTLALKGEVKDAYKGTAYGLTMDEKT
                     QKIYVGGRDYINEIDAKNQTLLRTIPLKDPRPQITSVQNLAVDSASDRAFVVVFDHDD
                     RSGTKDGLYIFDLRDGKQLGYVHTGAGANAVKYNPKYNELYVTNFTSGTISVVDATKY
                     SITREFNMPVYPNQMVLSDDMDTLYIGIKEGFNRDWDPDVFVEGAKERILSIDLKKS"
     gene            6279..6629
                     /locus_tag="OO130_003172"
     CDS             6279..6629
                     /locus_tag="OO130_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR5366474.1"
                     /translation="MPSGRHTARIRTGHDSGTVSPFVHSTLSPLVPVYPVFITVIADH
                     DKPQPSGCLLESQGSLCPICRQRITHETGWNVHHKVKKVMGGGEELSNLVLLHPNCHR
                     QLHSCEAGSLKKGL"
     gene            6772..6993
                     /locus_tag="OO130_003173"
                     /pseudo
     CDS             6772..6993
                     /locus_tag="OO130_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(7014..7076)
                     /locus_tag="OO130_003174"
                     /pseudo
     CDS             complement(7014..7076)
                     /locus_tag="OO130_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            7527..8939
                     /gene="ospC1"
                     /locus_tag="OO130_003175"
     CDS             7527..8939
                     /gene="ospC1"
                     /locus_tag="OO130_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC1"
                     /protein_id="HCR5366475.1"
                     /translation="MNISETLNSANTQCNIDSMDNRLHTLFPKVTSVRNAAQQTMPDE
                     KNLKDSANIIKDFFRKTIAAQSYSRMFSQGSNFKSLNIAIDAPSDAKASFKAIEHLDR
                     LSKHYISEIREKLHPLSAEELNLLSLIINSDLIFRHQSNSDLSDKILNIKSFNKIQSE
                     GICTKRNTYADDIKKIANHDFVFFGVEISSHQKKHPLNTKHHTVDFGANAYIIDHDSP
                     YGYMTLTDHFDNAIPPVFYHEHQSFLDKFSEVNKEVSRYVHGSKGIIDVPIFNTKDMK
                     LGLGLYLIDFIRKSEDQSFKEFCYGKNLAPVDLDRIINFVFQPEYHIPRMVSTENFKK
                     VKIREISLEEAVTASNYEEINKQVTNKKIALQTLFLSITNQKEDVALYILSNFEITRQ
                     DVISIKHELYDIEYLLSAHNSSCKVLEYFINKGLVDVNTKFKKTNSGDCMLDNAIKYE
                     NAEMIKLLLKYGATSDNKYI"
     gene            complement(9043..9191)
                     /locus_tag="OO130_003176"
                     /pseudo
     CDS             complement(9043..9191)
                     /locus_tag="OO130_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            9267..10964
                     /gene="ospD3"
                     /locus_tag="OO130_003177"
     CDS             9267..10964
                     /gene="ospD3"
                     /locus_tag="OO130_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspD3"
                     /protein_id="HCR5366476.1"
                     /translation="MPSVNLIPSRKICLQNMINKDNVSVETIQSLLHSKQLPYFSDKR
                     SFLLNLNCQVTDHSGKLIVCRHLASYWIAQFNKSSGHVDYHHFAFPDEIKNYVSVSEE
                     EKAINVPAIIYFVENGSWGDIIFYIFNEMIFHSEKSRALEISTSNHNMALGLKIKETK
                     NGGDFVIQLYDPNHTATHLRAEFNKFNLAKIKKLTVDNFLDEKHQKCYGLISDGMSIF
                     VDRHTPTSMSSIIRWPNNLLHPKVIYHAMRMGLTELIQKVTRVVQLSDLSDNTLELLL
                     AAKNDDGLSGLLLALQNGHSDTILAYGELLETSGLNLDKTVELLTAEGMGGRISGLSQ
                     ALQNGHAETIKTYGRLLKKRAINIEYNKLKNLLTAYYYDEVHRQIPGLMFALQNGHAD
                     AIRAYGELILSPPLLNSEDIVNLLASRRYDNVPGLLLALNNGQADAILAYGDILNEAK
                     LNLDKKAELLEAKDSNGLSGLFVALHNGCVETIIAYGKILHTADLTPHQASKLLAAEG
                     PNGVSGLIIAFQNRNFEAIKTYMGIIKNENITPEEIAEHLDKKNGSDFLEIMKNIKI"
     gene            11081..11200
                     /locus_tag="OO130_003178"
                     /pseudo
     CDS             11081..11200
                     /locus_tag="OO130_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355812.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
BASE COUNT         3447 a         2407 c         2394 g         2997 t
ORIGIN      
        1 gatgtgctgt ttcagcctcg cgaccagctc acgctgtgtc agcgtttccg tcgccgtttc
       61 tcccgttttg tggtccaggt aacggaagct caggctcgcc cctccgttgt aatgtgccag
      121 ccgggaggcc gctatcggcg gcttcttcag ataacgaccc aggtagcgcg ccgtattcct
      181 cccgccggcc attttcttcg acatgtacat caggcgcgga actcatcggg gcgcgggaat
      241 gaaatcccat tcagactccg gatagattca agcaagccca cttatcgtca aaatcggtgt
      301 tgcaaaaacg ggagtgacca tagatccgtt ttccaagcgg accccataat atcggctggt
      361 aaacatcact cgctagtgca ttctttttgt tggtaatgat aattatttac atttattctt
      421 tgatgtttta tcataattaa tgttttatca ataaatatat ggaaatatat tgtttcagat
      481 gggaatatga gaaagttcat ccttatttct atgataacgt tagctagtgt tagtctggga
      541 gcctgcagac agaatgtcac cataaagcag gatgcgccag ggcagaaagc tttcctgatt
      601 gacgggcaga tttacgattt gcacagtggc aaaattattt ctagcagcga gcttttggcg
      661 gatcttgcta ccgctcgaca tcttattatt ggtgaaaaac acgataatgc cgagcatcat
      721 cagattgagc tttggcttat tcaaaatctg ctcatccagc gaccacaggg gagtgtgttg
      781 cttgagatgt tgacgtcaga ccaacagccc cgagttaacc aagttaaatg ttggctgaaa
      841 gataatcctg tggttcgaga tagccgtgtt caggaactac ttaactggca aaaagggtgg
      901 tcctgggaga tgtatggcga catcgtcatg cagctattac gtgggcctta cccgctgctc
      961 aatgccaaca ttggccggga gcagatgttg gcactgtaca aaaaaaacga atttcctaaa
     1021 ggaaaaaaat ccactgcacc tgtggtacaa gaagctctgc gtgaaaccat catagctatg
     1081 catgaaggca accttgaaag ccagcaactg acgtcaatgc tctctgttca gcagcagcgg
     1141 gatcgctaca tggcccgcca gcttctgagt gccccggtac cgtcgttgtt gattgcaggt
     1201 ggttatcacg cgtcaaaaaa catgggcgta ccgttacaca tggaagatct tgccaccggc
     1261 acccatccag tggtgttgat gcttgcagaa aaaggaatga acatcacagt agatcatgct
     1321 gactacgtgt ggtttgtagc gccagataca acaaaaaggt aaaccatgga tgatgaatat
     1381 tctccacttc ccacagtcgg taaaatggag cagctggttt atctgttctc tactgcttca
     1441 tggactgatt ttccttacgt ttatctggcg tttttccgaa gttcagcccg ctatgtctcc
     1501 tgcaccggcg atcatgctgc aatgggcaga aattatagag gctccttctt ctccactttc
     1561 gcttccggtg ggaatcgctc aacaggagtc cgcagtgaca gaagaaaagc aacaaactga
     1621 ggacagacaa caacgtcctg tcactgaaga cagtgatgca accattgaaa taactaggaa
     1681 gaaaaagtca tccgatggag aaaagaaaaa gacacgtcca cctcgtaaga taaaggctca
     1741 aaccagcgac agcaacccca cagccgtttc cagtaatgct gcacctcagg ctctggtcga
     1801 atcgtcccgt attgctgcgc cttttaacag tgactcaaca aagcgagata attctgaggc
     1861 atcttgggaa agcagagtaa aaggccacct taaccgttat aaacgttacc ctggcgatgc
     1921 cagaaaacgt gcacgaaccg gcacagccgt tgtgactttt accgtaaaca cggaaggcac
     1981 tattgtcagt agtttcttgg aaatctcgtc aggaaccctt tctcttgacc gcgaagctat
     2041 tgctgtttta gaacgcgctc aaccacttcc caaaccaccg cctgaaatat tggagggagg
     2101 attatttaaa gtaaaaatgc cgattacctt taagttgaag gagtaaaggg ataattggcc
     2161 gttcaagttc atttaactga aatttaattt tcatttttat gctgaacatt ttatcaaact
     2221 tttccactgt cgaaaaaatc aaaaagacag tagatcataa ttaataaaca aatagttata
     2281 cttatcttta ttaatattat atccaaggaa atttatgaac gttataaaac tggctatcgg
     2341 ctcaggcata ttattgctca gctgcggtgc ttactcacaa tccatcagtg aaaaaactaa
     2401 ttccgacaaa aaaggagcgg cagaattcag tccgctcagc gtttctgtcg ggaagacgac
     2461 cagtgagcag gaagctctcg agaaaacagg cgcgaccagt tcccggacaa cggacaaaaa
     2521 cctgcaatca cttgacgcaa cagtgcgtag tatgcctggt acttatactc aaatagatcc
     2581 tggtcaggga gcaatcagtg tgaatattcg aggcatgagc ggatttggtc gtgtaaacac
     2641 tatggtcgat ggtattaccc agagttttta cggaacctct acctccggaa caacggcgca
     2701 tggttcaact aacaatatgg ctggcgtact tatagatcct aacttactgg tagcagttga
     2761 tgttacacgc ggtgacagca gtggctctga agggatcaac gcccttgccg gtagtgcaaa
     2821 tatgcgtact attggcgttg acgatgtaat atttaacggt aatacatatg gccttcgttc
     2881 acgtttctct gtcggtagta atgggctggg acgcagcgga atgatcgccc ttggtggaaa
     2941 aagcgacgct tttacggata cgggaagcat tggcgttatg gctgctgtga gtggcagttc
     3001 tgtgtactct aatttctcaa atggttctgg aattaacagc aaagagtttg gttatgataa
     3061 atatatgaag cagaacccca aatcccaact gtataaaatg gatatcagac cagacgaatt
     3121 taacagcttc gaactttccg ctcgaaccta tgaaaataaa tttacacgtc gtgatataac
     3181 cagtgacgac tattacatta aatatcatta cacccctttt tctgaattaa ttgactttaa
     3241 cgtaacggcc agtaccagtc gcggtaatca aaagtatcgt gatggctcgc tgtatacttt
     3301 ctacaaaacc tcagcgcaaa atcgttctga cgcgctggat atcaacaata ccagccggtt
     3361 cactgtcgcg gacaatgaac tggagtttat gctgggcagc aaactgatgc gtacccgcta
     3421 tgaccggacc attcactcag cggcgggcga cccgaaagcg aatcaggaat cgatcgagaa
     3481 caatccgttc gcaccctccg gccagcagga tatttcagcg ctgtataccg ggctgaaggt
     3541 tacgcgcggc atctgggagg cagatttcaa tctcaactac acacgtaaca ggatcacagg
     3601 gtacaagccc gcctgcgatt cacgcgttat ctgcgtgcca cagggtagct acgatattga
     3661 cgataaagag ggtggcttca acccttcagt tcagctttct gctcaggtaa caccatggct
     3721 tcagccgttc attggctaca gcaaatccat gcgcgccccg aacatccagg agatgttctt
     3781 ctctaattca ggaggcgcat ccatgaaccc attcctgaag cctgaacgtg cagaaacctg
     3841 gcaggcgggt tttaacattg ataccagaga tttactggtc gaacaggatg ccctgcgctt
     3901 taaggctctg gcgtaccgca gcaggatcca gaactacatc tacagcgagt cttatctggt
     3961 ttgttctgga ggtcgtaaat gcagtatggc tgaggtgatt ggcaatgact gggagggcat
     4021 tagcgatgaa tacagcgaca atatgtacat ctacgttaac tcggcaagcg acgttattgc
     4081 aaagggcttc gaactggaga tggattatga tgcaggtttt gcttttggcc gactctcttt
     4141 cagccagcag caaacagacc agccaacctc catcgccagc acctactttg gcgcagggga
     4201 tatgaccgaa ctgcccagaa aatacatgac gctggatact ggtgttcgct tcttcgataa
     4261 cgcgttgacc ctgggcacta tcataaaata cacaggcaag gctcgtcgcc tgtcgcctga
     4321 ttttgagcag gacgaacata ccggcgcaat aatcaaacag gatttgccgc agatcccaac
     4381 gattatcgat ctctatggta cttacgagta caaccgcaac ctgacactga aactttcggt
     4441 acaaaacctg atgaacagag attattcgga ggcgctgaat aagctcaaca tgatgccagg
     4501 tcttggtgac gagagccacc cagccaattc cgcgcgtggc agaacatgga tatttggcgg
     4561 ggacattcgt ttctgatgac aatttccgcc aggttcagac aatacgtttt ctctcttatg
     4621 tcaattttat tgcaggaacg aaaaatgaat attttcactt tatccaaagc accgctatac
     4681 ttgttaattt cactattttt acccacgatg gccatggcta tcgatccacc tgaacgcgaa
     4741 ctttcgcgat ttgccctgaa aacgaattac cttcagtccc ctgatgaagg cgtctatgaa
     4801 ctggcgtttg ataatgccag taaaaaggtg tttgcagcag tcaccgatcg tgtaaatcgt
     4861 gaagccaata aaggctatct gtattcgttt aattcagatt cgctgaaagt cgaaaataaa
     4921 tacacgatgc cataccgggc attttcgctg gcgataaatc aggataaaca tcagctctat
     4981 atcggacaca cccagtcagc gtccctgcgt atcagtatgt ttgacacccc aaccggtaaa
     5041 ctggtaagaa ccagcgacag gttaagtttt aaagcggcaa acgctgcaga ttcgcgtttt
     5101 gagcatttgc gccatatggt ttacagccag gattccgata ccctgtttgt gagttatagc
     5161 aatatgctga aaacggccga gggcatgaag cctctgcata agctgttaat gctcgacggg
     5221 acgacgcttg ccttaaaagg cgaggttaag gatgcttaca aaggtacagc gtatggtctg
     5281 acgatggatg aaaaaacaca gaaaatctac gttggcggaa gagattacat caacgaaatt
     5341 gatgcgaaaa atcagacgct gctgcgtacc atcccgttga aagatccgag accacaaatc
     5401 acaagtgtgc agaatctggc ggtggactcc gcttctgacc gtgcctttgt ggtggtattc
     5461 gaccatgacg atcgttccgg tacaaaagat ggcctctata tttttgactt acgcgacggt
     5521 aaacagcttg gctatgtgca cacaggagcc ggagctaacg cggtgaaata caatccgaaa
     5581 tataacgaac tgtatgtcac caacttcact agcggcacca tcagcgtagt ggatgccacc
     5641 aaatacagca tcacccgtga atttaacatg ccggtctacc caaaccagat ggtgttgtcg
     5701 gacgatatgg atacccttta cattggcatc aaagaaggct ttaaccgcga ttgggatcct
     5761 gatgtgtttg tggaaggagc taaagaacgt attctgagca ttgatttgaa aaagtcgtga
     5821 tcgggggaaa aaacatggga taaaaactcc gttattccac taaactggcc ggactaggaa
     5881 aagcaaacat ctctgtcctg ccggtacgcc tgttacgccg ctccatgagg ctgaggaatc
     5941 tccagaaaac gagacaggca cagcattgta cgatcaactg atttgtgcca ataattcaat
     6001 gaaatcacag ctatgcctgt tcacgtatag gaaattgaaa aactaagcga taccactgtc
     6061 cgcgtcttat gcctcgtgcg gtaagctgtt tttcacatca cgccggtgtt tttttctttc
     6121 tgtggacggt tttaatactg taatcggccg ttttttatac ttaccccgat tcccgggcgc
     6181 acttatcctg catcaccccg ggatatacct cagcagactg atatcccgcg cgtttttctt
     6241 cagcccctcc acattcagtc ccgcgatggc ccggcggtat accatccggc cgccacacag
     6301 cacgcattcg aacgggtcac gactcaggaa ctgtttcacc atttgtgcat agcacacttt
     6361 cgccactggt tccggtttat cctgttttta taacggtaat tgcagaccat gacaaaccgc
     6421 agccgtcagg ctgcctactc gagtcacaag gctctctgtg tccgatatgc cggcagcgca
     6481 ttacccatga aactggctgg aacgtacacc ataaggtgaa gaaagttatg gggggcggtg
     6541 aagaattatc taaccttgtc ttgttacatc caaattgcca tcgacagtta cacagttgtg
     6601 aagccggctc tctcaaaaag ggcttataaa ggcttgagcg gtgtaccggg aaaccggtat
     6661 gcaccgttct taggggaggg cagtccagca atgaactgtc cttacccgac aaacatccaa
     6721 aacgaacgga acgtgctgga atccgcatat aacccgttgt aaagtgagga aatggacacc
     6781 tcacttgctc atgagaacgc ccgcctgcgg gcactgttgc agacgcaaca ggacactatc
     6841 caccagatgg ctgaatacaa ccgcctgctc tcacagcgga tggcggctta tgcttccgaa
     6901 atcaaccgac tgaaggcgct ggttgcgaaa ctgcagcgta tgcagttcgg taaaagccca
     6961 gaaaaacttc gcgcaaaaac cgaacggcag attccatttt cacgggcgat ctacgccaca
     7021 caggctctgg gttgcagtga ctgctccatt aaggcgattt tgaactcata agggaagtaa
     7081 caaaaaaact gcaaaagtac aataattata tatggccaaa ctaacctcta ttaaaaaaac
     7141 atcagttttt cacacttagt tgagcttcat gaaaaaatca tcgaggattt cattgcagaa
     7201 tacacattcc actaactgga agcactccat aagaggaatc agagaaagta tcgttttttt
     7261 atagtaaaat tcattgctgt tcaataaaat tgatataatt attaaattat ggaaataatt
     7321 tcacaaaaaa gattaacttg agttcttaag catcggattt tagatatttt gaaatatctg
     7381 gcatcacata acatcaaaag tatataatac ccttatctaa ttacatgcag tatattgtat
     7441 atatactcaa aaattgtaat tcatacccta ctatatcttg tggggaaaga tattaattaa
     7501 aactgttttc atataaggtt cattttatga atatatcaga aacactgaac tcagcaaata
     7561 cccaatgcaa tatagattct atggataaca gattacatac attgtttcca aaagtgacat
     7621 cagtgcgaaa cgctgcacaa caaactatgc cagatgaaaa aaatttaaaa gatagtgcaa
     7681 atattattaa agatttcttt aggaaaacta tagcagcaca aagttatagt agaatgttct
     7741 ctcaaggctc taactttaaa tctttaaata tagcaattga tgcaccatca gacgctaaag
     7801 cctcatttaa ggctattgag caccttgaca gattatcgaa gcattatata tctgaaataa
     7861 gggaaaaact tcatcctctt tctgcagagg aactcaattt gctttcgcta attattaatt
     7921 ctgatttaat cttcagacat caaagtaatt ctgatttgtc tgataaaatt ttaaacatta
     7981 agtcattcaa taaaattcag tctgaaggaa tatgcacaaa acgaaacaca tacgctgatg
     8041 atataaaaaa aatagctaat catgactttg tgttttttgg cgttgaaatc tctagccatc
     8101 agaaaaaaca ccccctgaat acaaaacatc acactgttga ttttggtgca aatgcgtata
     8161 tcattgatca tgactctcca tatggatata tgacattaac cgatcacttt gataatgcta
     8221 ttccacctgt tttttaccat gagcaccaat catttttaga taaattttca gaggttaata
     8281 aagaagttag tcgatacgta catggaagta aaggaattat agatgtacca atattcaata
     8341 ctaaagatat gaagttaggg ctcggattat acctgattga ctttattaga aaaagtgaag
     8401 accaaagctt caaggagttt tgctatggaa aaaatcttgc ccctgtggat ctggatagaa
     8461 tcataaactt tgtttttcag ccagagtacc atatacctag gatggtaagt acagaaaact
     8521 tcaaaaaagt taagattaga gaaatatcct tagaggaggc tgttacagca tctaattacg
     8581 aagaaattaa caagcaggtc actaacaaaa aaattgctct ccagactctt tttctttcga
     8641 ttactaatca aaaagaggat gtcgccttat atatattatc taattttgag ataactagac
     8701 aagatgttat ttccataaag catgagttgt atgatattga gtatctactt agcgctcata
     8761 attcaagctg taaagtactt gagtatttta tcaataaggg attggttgat gtaaacacaa
     8821 agttcaaaaa aactaatagt ggggattgta tgttggataa cgcaataaaa tatgagaatg
     8881 cagaaatgat aaaactatta ttgaaatatg gtgcaacatc tgacaataaa tatatttaat
     8941 caaaattgaa tatcgtttag caaaaggcag tatttgcaca aaatatcgat atattgttta
     9001 ttgtcagtat ggctcaatgt gataatagtt ggaaagtttg atgggtttcg ccccgttgta
     9061 gcggtagtcg agctggaagg tcttcaggca ctgcttacag cgatagagca gcccccctga
     9121 actggaatgg ccgttccgga taccccctga gtttcagagt aacggggaca aaccacatca
     9181 atctttgcca tcaatcatcc aaagggcaaa gagtacaaca acactaagtc tgcgtcacaa
     9241 cccatcaatg aaaggaatat atacatatgc catcagtaaa tttaatccca tcaaggaaaa
     9301 tatgtttgca aaatatgata aataaagaca acgtctctgt tgagacaatc cagtctctat
     9361 tgcactcaaa acaattgcca tatttttctg acaagaggag ttttttatta aatctaaatt
     9421 gccaagttac cgatcactct ggaaaactta ttgtctgtcg acacttagct tcctactgga
     9481 tagcacagtt taacaaaagt agtggtcacg tggattatca tcactttgct tttccggatg
     9541 aaattaaaaa ttatgtttca gtgagtgaag aagaaaaggc tattaatgtg cctgctatta
     9601 tttattttgt tgaaaacggt tcatggggag atattatttt ttatattttc aatgaaatga
     9661 tttttcattc cgaaaaaagc agagcactag aaataagtac atcaaatcac aatatggcat
     9721 taggcttgaa gattaaagaa actaaaaatg ggggggattt tgtcattcag ctttatgatc
     9781 ccaaccatac agcaactcat ttacgagcag agtttaacaa atttaactta gctaaaataa
     9841 aaaaactgac tgtagataat tttcttgatg aaaaacatca gaaatgttat ggtcttatat
     9901 ccgacggtat gtctatattt gtggacagac atactccaac aagcatgtcc tccataatca
     9961 gatggcctaa taatttactt caccccaaag ttatttatca cgcgatgcgt atgggattga
    10021 ctgagctaat ccaaaaagta acaagagtcg tacaactatc tgacctttca gacaatacgt
    10081 tagaattact tttggcagcc aaaaatgacg atggtttgtc aggattgctt ttagctttac
    10141 aaaatgggca ttcagataca atcttagcat acggagaact cctggaaact tctggactta
    10201 accttgataa aacggtagaa ctactaactg cggaaggaat gggaggacga atatcgggtt
    10261 tatcccaagc acttcaaaat gggcatgcag aaactatcaa aacatacgga aggcttctca
    10321 agaagagagc aataaatatc gaatacaata agctgaaaaa tttgctgacc gcttattatt
    10381 atgatgaagt acacagacag atacccggac taatgtttgc tcttcaaaat ggacatgcag
    10441 atgctatacg cgcatacggt gagctcattc ttagcccccc tctcctcaac tcagaggata
    10501 ttgtaaattt gctggcctca aggagatatg acaatgttcc cggacttctg ttagcattga
    10561 ataatggaca ggctgatgca atcttagctt atggtgatat cttgaatgag gcaaaactta
    10621 acttggataa aaaagcagag ctgttagaag cgaaagattc taatggttta tctggattgt
    10681 ttgtagcctt gcataatgga tgtgtagaaa caattattgc ttatgggaaa atacttcaca
    10741 ctgcagacct tactccacat caggcatcaa aattactggc agcagaaggc ccaaatgggg
    10801 tatctggatt aattatagct tttcaaaata ggaattttga ggcaataaaa acttatatgg
    10861 gaataataaa aaatgaaaat attacacctg aagaaatagc agaacacttg gacaaaaaaa
    10921 atggaagtga ttttctagaa attatgaaga atataaaaat ctgaatatta tctcgtggtg
    10981 atacactgaa tttgaggtaa ccggctcatt taaactgtct ggtctgttac ctccggttcc
    11041 acaaaaataa tgtccatcat tttgaagtgg acactatcgt atggaatacc ggacctggat
    11101 cactgaagct ttacgccttc atttcgaaga acatttacca cgggttgtgg ccggacgtcg
    11161 cctgggcgta ccaaaatcaa ctgtctgtgg tatgttcgtg tgaaccgccc ccggtatccc
    11221 gctcatgctg gcgaacccag acacg
//
LOCUS       DAJTMC010000136        11228 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.136, whole genome shotgun sequence.
ACCESSION   DAJTMC010000136 DAJTMC010000000
VERSION     DAJTMC010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11228
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.136"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            55..653
                     /locus_tag="OO130_003179"
                     /pseudo
     CDS             55..653
                     /locus_tag="OO130_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905087.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(682..1170)
                     /locus_tag="OO130_003180"
     CDS             complement(682..1170)
                     /locus_tag="OO130_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001761054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5366477.1"
                     /translation="MMMIYGMFVFELRTLPHQQLQQNKSWRHVKNERVNRSASWQYIG
                     AGDDRIVLSGVLYPEITGGEVSLSLLTTQAYTGRPWPLIDGVGQIYGMYVLTETNTTR
                     SEFDRYGKAKKIEFSLTLERCDEDLRERLQSSSFSDMLSGFKDKVTSSLNSAASSVKG
                     LF"
     gene            complement(1183..3990)
                     /locus_tag="OO130_003181"
     CDS             complement(1183..3990)
                     /locus_tag="OO130_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000853438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR5366478.1"
                     /translation="MKTLDIRVAFSAVDRLTRPAENARRLMGQFGDSIQRTQGAIKNL
                     ERQARSFERARDAVSKADAGIVKARRQLNALNQLQRTGTVLSEKQQKLMQQLSTRLER
                     LNESRTREIQKMRELGGELKRHGISLTGSDNTIQQAIRRTEQYNNQLERERQALARVT
                     RARERYSRAQETAGKLKTGGALAIGAAAAGGYAAGRFLQPAIGFGKEMSRVQALTRID
                     QNSPQFKALREQALKLGSETQFTAGDAASGQAFLAMAGFTPQAIQAALPGVLSMATAG
                     GMDLGETADIGSNILTQFGLSADQMDRVGDTLTAAFTRTNTDLRALGETMKYAGPVAG
                     KLGISLEQAAAMAGVLANMGIRGSDAGTAMRASLARLASPPKAAAEALKELGVSVSDA
                     GGKMRPMEDVLADLYKATRKYGEVDRVSFFKDIAGEEAFTSFMALVDAAGDGSLPKLR
                     KELEGARGEAERTAKVMANNLDGDLKSLSSAWEGLRIRIADLIDGPLRSVTQWLTRVV
                     SKVTALAQAHPALTRQLLIAGGALLAMTATVGSLSLAIGVLAGPLAKLRLGFSLLTGS
                     MNAVRVLPALWGMVTGSVSLLGGAIGALFSPVGLIVAALAGAAVLIWKYWDPIRAFFA
                     GVFSGIMERLTPLRETFERFGPVFDAIGSGISQVFNWFKSLLSPMESSKETLDKCTSA
                     GEIFGNVLGGALQLVLTPAKMLLDTLAWILEKLGVLPDEAERARKKIEDAQRAAILQD
                     KVALLQGDLAKINPPKPVENGNGTGGDNPKDNKPLTDSNTGTLRRLSKIADNTGKLVE
                     ETKKRIGPGDIVFKNLPRALAVRGEWQERKIAQVSKPAPAINITPVVPAPLPPALVPV
                     VAASSRPVAEAIRSPVASVPVTSRNREPVAFGFGGEIHVHLHNVVTQNPRELAKLVGE
                     MVRAEMERRARAGRGCFYDKD"
     gene            complement(4028..4132)
                     /locus_tag="OO130_003182"
     CDS             complement(4028..4132)
                     /locus_tag="OO130_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HCR5366479.1"
                     /translation="MCFEHIEDLVADIAAIFNWSPAEIFMMTPGEVVS"
     gene            complement(4141..4515)
                     /locus_tag="OO130_003183"
     CDS             complement(4141..4515)
                     /locus_tag="OO130_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5366480.1"
                     /translation="MKDIDTETRNNTVADNVTAVEDMAVERGVKLTRPIERGGEKITY
                     VEITGAIEQAGSLRGLSLSDVLNLKADTMFTLLPRVTSPRLDEVMIKKMSSRDFIQLC
                     AVAVNFMSEPDSGAKSVQETAA"
     gene            complement(4571..5081)
                     /locus_tag="OO130_003184"
                     /pseudo
     CDS             complement(4571..5081)
                     /locus_tag="OO130_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310671.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
     gene            complement(5081..6265)
                     /locus_tag="OO130_003185"
     CDS             complement(5081..6265)
                     /locus_tag="OO130_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath family protein"
                     /protein_id="HCR5366481.1"
                     /translation="MSETRFHGARVTENTDLVTAINDVDSSVIGIVATADDADAKLFP
                     LNKPTLLTRVNDVLGKCGTTGTLYRALKAIADQVSTKVIVVRVAEHKEEDGKTQDQLV
                     IGGSEDDGSYTGMYALLVAEQDESIGYRPRILAAPELDTEAVTKSLCVIADKLRAFVY
                     ASCHGCNTMAEAITYRQKFNEREVMLLWPDFIAYNPKNGKNETFPAPAYACGLRAYID
                     HEQGWHKSLSNVPVKNVLGMSRHVFWSLQAEDSDANSLNNKEITTIIRRNGFRFWGNR
                     TPETNAYIFEVYTRTAQVLADSIAEAQFETIDSPLTPANVKDVISAIRAKLDSLVTAG
                     KLIGAECWYDVVDNSTTDLRQGRVRIRYKYTPVPPLEDMELYQTFTDEYFEPAFAVLG
                     GA"
     gene            6423..7532
                     /locus_tag="OO130_003186"
                     /pseudo
     CDS             6423..7532
                     /locus_tag="OO130_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494339.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
     gene            7781..10900
                     /locus_tag="OO130_003187"
     CDS             7781..10900
                     /locus_tag="OO130_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5366482.1"
                     /translation="MIGQFLSATEILAKNYVRNKMVKNPFYSNLKWNFIEKNIIRLTS
                     SPVKSVLCISAFSFVLLYVGYLNELFIKNNLLHYFPFRHSLTEWQTTILSGQLTIIGI
                     VYPLVIGLVSVLFQKKADRKIAQTAYQRYSGFMLAGLSGLFLSGFILLSVLIKTVFGS
                     YLYGIACLISILWLLINIVLSIWFFIVSLEILDDVKRQIIIKRYIAFEIVMPHICNKI
                     SAKLRLYPIYQKHNYSNLEITQADYKGEYISVASSYSKEDELSLYHRPFQLTLNLINY
                     QLKKKNHFASFVIGDNRTKETESTGKILFSVKNIKPDSLLIKILKQCFYRAPIKGGDF
                     SVSLTMQAITADTYMYLRDSDLISFDNAISALINNFNNLCDLYFFQDDNTNNNFLLIT
                     TELFERSFQYEFSDEVYKISNNSMDKINLSERFFELCLWSGVRIINNRKHLISNELCI
                     YMGITRSQWSILTEWFRNNQSLLNASLRSRYNRILRTYITVWEQYQESINFRFCNTEN
                     SDLFELFCKTQLQELPSMIIDATQTRDPSTIDTAVDLINRWQHSMNIDSHSVEKYSYK
                     GQLFNPGFFISKKLNFNSDREWFNIAIINALTDMRICTCLYLTSRINTSDKLMTHYIK
                     LILEGKLIDQTGGYETPTEEIDNASQLIKILIRICLWTWSENMEHNGWMNSLARRLRD
                     YDKTDMVMGRVYSNVFDCGFIDMEQSWVQLLLIFSNKNDSVSKEIKEAIENNYITYRE
                     KQRLIGVLSKICNSIEYTKIKLTLTLDDLQTKKENLRKLLQEHINMLKKDLDMRLQDA
                     AIDVHRLDSTARKTSEHLRKRIKKTLPLSLFKSIDFKQASDCFTKHKISIKIDKEPYA
                     EGIESIPYINEGDIQADLILKDIQRIILSNLFSTGCSQHTVIEDFNMLIDHIKSSADL
                     AGKLVLVMSKEIFQQYNRMLFDNPNLRELMRKNDDGSMNITTESGTRKVYFLPFVNQP
                     FSLVVKDNYFTKLIIREYDNNKLVNVTSENIKSDSDKFKLTLNYELNIVFEGNADLKI
                     AHSQRVTSE"
     gene            11036..>11228
                     /locus_tag="OO130_003188"
     CDS             11036..>11228
                     /locus_tag="OO130_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001512982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HCR5366483.1"
                     /translation="MFRCPLCGASARIRTSRPENDSNTVRQKYYQCNNLECGVCFSTL
                     EAFHKFTSKHASPIHSSEGI"
BASE COUNT         3071 a         2817 c         2515 g         2825 t
ORIGIN      
        1 tggccgtttc tggcctgcat ggcatgattt gcgcttttgg acgggagatt cagtgtgctg
       61 attggctatg taagggtatc aacaaatgac cagaatacag acctgcaacg aaacgctctt
      121 gtttgtgcag gatgtgaaca aatatttgaa gataaattaa gcgggacaaa gacagaccga
      181 ccgggattaa aacgcgcttt aaagcgcctt caaaaggtga cacgctggtt gtctggaaac
      241 tggatcgcct cgggcgaagc atgaaacatc tgatttctct cgtcggggaa ctacgggagc
      301 gagggattaa ttttcgcagt ctgaccgaca gcatagacac atcttctcca atggggcgtt
      361 ttttcttcca cgtgatgggt gccctggctg aaatggaacg tgaattaatt gttgaacgta
      421 cactggccgg gctggcggca gcgcgcgcaa gggggcgcac aggcggacgt cgaccgaagc
      481 tgacaaaaga acagcatgag caaatagcaa ggctaatcaa aaacgggcac gacagaaaac
      541 aactggcaat aatttacggc attggcacat cgacgattta tcgttatcac cctgtaggcg
      601 atatacagac tgaagaaaca accgggcaga ctcaggaaaa tgaaaaccgc taatctgacc
      661 attagcggtt tttgtgttaa atcagaacag ccctttaact gaactggccg cgctgttaag
      721 agatgatgtc accttatctt tgaagccgga cagcatatca ctgaacgatg aggattgcag
      781 gcgctcccgc aaatcctcat cacagcgttc aagagtcagt gaaaattcta tctttttcgc
      841 cttaccgtag cgatcaaact cggagcgggt cgtattcgtt tcagtcagga catacatgcc
      901 gtaaatctgc ccgacgccat caatcaaagg ccagggtcgt cctgtatacg cctgcgtagt
      961 cagcagcgac agcgacactt cgccacctgt aatttcagga taaagcacac cagaaagaac
     1021 gatgcgatca tcacctgcac cgatatactg ccagcttgct gaacggttaa cgcgttcatt
     1081 tttcacatgc cgccagcttt tgttttgctg taactgctga tgcggcagcg tgcgcagctc
     1141 aaaaacaaac atgccgtaga tcatcatcat ggccatgact cctcaatctt tatcgtaaaa
     1201 acagccacgc ccggcacggg cgcgccgttc catttctgcc ctgaccattt caccgaccag
     1261 tttcgccagt tcgcggggat tctgcgtaac aacgttatgc agatgaacat gaatttcacc
     1321 accaaatccg aaggcaacag gctcccggtt acgggaagtt acaggaactg atgccactgg
     1381 agatcgtata gcctccgcca ccgggcggga gctggccgca acaacaggga ccagcgccgg
     1441 aggcagcgga gccggaacca cgggtgtgat attaattgcg ggggcaggct tactgacctg
     1501 cgcaatcttc cgctcctgcc actccccacg aacagcaagt gctcggggca ggtttttaaa
     1561 gacaatatcg ccggggccaa tgcgtttttt cgtctcctca accagcttac ctgtgttatc
     1621 agcaattttg ctgagtctgc gcagcgtacc ggtattgctg tctgtgagcg gtttattgtc
     1681 tttggggtta tcacctccgg tgccattgcc attttccaca ggcttcggcg gattgatttt
     1741 cgccagatcc ccctgaagca aggcaacctt gtcctgaaga atggccgcac gctgtgcgtc
     1801 ttcgattttc ttgcgcgccc tttccgcttc atccggaagc acaccaagct tttcaagtat
     1861 ccacgccagc gtatccagca acatttttgc aggtgtcaga acaagttgta acgcaccgcc
     1921 aagaacgtta ccgaatatct cgccagcact ggtacattta tccagagttt ccttgctgga
     1981 ctccatcggt gacagcagcg atttaaacca gttaaacacc tggctgatcc cactcccgat
     2041 tgcgtcaaaa acaggaccaa accgttcaaa ggtttcgcgc aacggggtca gcctttccat
     2101 aatcccgctg aacaccccgg caaaaaatgc cctgatggga tcccagtatt tccagataag
     2161 aacggcagct ccggcaagcg cagccacgat aagaccaacc ggactgaaca acgccccgat
     2221 agcgcctccc agtaaagaaa cggaacccgt caccattccc catagtgctg gcagaaccct
     2281 gacagcattc attgatccgg tcaggaggga aaaaccaaga cgcagttttg ccagcgggcc
     2341 agcaagcaca ccaatagcca gcgacaacga gccaaccgtt gcagtcattg ccagcaatgc
     2401 accgcctgca atcagtagct ggcgcgtcag tgcgggatgg gcctgcgcca gcgccgtcac
     2461 ctttgatacc acacgcgtga gccactgcgt gacagaacgc agcggaccgt caatcagatc
     2521 tgcaatgcgg atgcgcaacc cttcccatgc actgctgagt gatttcagat cgccgtcaag
     2581 gttgttggcc ataacctttg ccgtgcgttc agcctcaccg cgcgcgcctt caagttcttt
     2641 tctcagtttg ggtaaggagc cgtcacctgc cgcatcaacg agggccataa atgatgtgaa
     2701 agcctcttct ccggcaatgt ccttaaagaa cgatacccgg tcaacttccc cgtatttgcg
     2761 ggtagcttta taaaggtcag ccagcacatc ctccatcggg cgcattttgc ccccggcatc
     2821 cgagacagac acgccaagct ctttcagcgc ctctgctgcc gcctttggcg gtgatgccag
     2881 acgagccagg ctggcacgca ttgccgtacc agcatcactc cccctgatac ccatattcgc
     2941 cagcacgccc gccatcgctg cggcctgctc cagagatatt cccagcttac ccgccaccgg
     3001 acctgcatat ttcatggttt cacccagtgc gcgaaggtca gtgttggtac gggtaaacgc
     3061 tgcggtgagt gtgtcgccga cccggtccat ctggtcagca gaaagaccga actgcgtcag
     3121 aatatttgag ccaatatccg ccgtctcacc gaggtccata ccgccagccg ttgccatgct
     3181 cagtacgcca gggagcgcag cctgaatggc ctgtggtgta aagccagcca ttgcaagaaa
     3241 tgcctgtcca ctggcggcat cgcctgcggt gaactgcgtt tcagagccaa gttttaacgc
     3301 ctgctcacgc agcgccttaa actgcgggct gttctggtcg attcgcgtca gtgcctgaac
     3361 gcgggacatc tctttgccga acccgatcgc aggctgcaaa aaacgcccgg cagcatagcc
     3421 gcccgccgct gccgcaccaa ttgccagcgc accacctgtt ttcagttttc ccgctgtttc
     3481 ctgcgcgcgc gaataccgct cacgcgcccg tgttacacgc gcaagcgcct gccgttcgcg
     3541 ttcaagctgg ttgttgtact gttcggtgcg tctgatggcc tgctggatgg tgttatcgct
     3601 gcctgtcagg gaaatgccgt ggcgtttcag ctctccgcca agctcccgca ttttctgaat
     3661 ttcccgtgtg cgcgattcat tcaggcgttc aagccgggtg cttaactgct gcatcagctt
     3721 ttgttgtttt tcgctgagca ctgtacccgt gcgttgtaac tgattaaggg cgttaagctg
     3781 gcgtcgtgct ttcacgatgc cagcatccgc tttactgaca gcgtcacggg cgcgctcaaa
     3841 tgatcgcgcc tgacgctcga gatttttgat cgccccctgc gttcgctgga tggagtcacc
     3901 aaactgcccc atcaggcggc gggcgttttc ggcaggccgg gtcagcctgt caacggcgct
     3961 gaaagcgacc cggatatcaa gagtcttcat tgtctgcatt cccgctacga agtgccgccc
     4021 gctcacgtca gctaaccact tcgccgggcg tcatcatgaa gatttcggcg ggcgaccagt
     4081 taaaaatggc ggcaatatcc gccaccagat cttcgatgtg ctcaaagcac accagggtga
     4141 ttacgctgcc gtctcctgca cgctcttcgc gccagagtct ggctcgctca taaaatttac
     4201 agccacagcg cacaactgaa taaaatcgcg tgacgacatt tttttaatca tcacttcatc
     4261 cagtcgtggc gaggtcacgc gaggcaacag cgtgaacatg gtatccgctt tcagattcag
     4321 cacatcagac agcgacagac cacgcaggga tccagcctgc tcaatagccc cggtgatctc
     4381 cacatacgtg attttttcgc caccacgctc aattggtcgg gtcagtttta cgccacgttc
     4441 gacagccata tcctcaactg ccgtcacatt atccgccacg gtgttattcc gggtttcagt
     4501 atcgatgtct ttcatctgtt gtctcctttt cagtcagagg cgacgcactg cgccgccagc
     4561 atattactta tcagccaagc ccaagcgcgg aacggatgcg atcgggcaca atgtccttgc
     4621 cgtccttccg gtaaatgaag ttcagcaggt caatctccca caacgggcga tcgttaacac
     4681 tcagcttgta gtaggtgttt taatggcgta agtgtgtgat gtggcttcgc cctgtttggc
     4741 ttcccccata tcaatttccg tcacacgtcc gcgcatttcg acttcataca ggtcgctttc
     4801 tgcatcggtg tagtattcac ccgcaaaacg cagcagcgtg ccgtcaatcg tgccgccata
     4861 cttaaggaac agctcacgaa ctgcgccccc atgacaaagc tcgcatcaag cgcggagtcg
     4921 tccagaccga gatcaatact taccgcaccc atcatgccac caccccggta gctgtcggtt
     4981 ttgcgcgtca gcttaggcag agtgacggac gtcacattac ccacttcgtt ttcaccatcc
     5041 acaaacagcg taaaaaagcg aagatgtttt ggcacagcca tcaggcacct cccagcaccg
     5101 caaatgcggg ttcaaagtat tcatcagtaa acgtctggta aagctccatg tcttccagtg
     5161 gcggaacggg cgtatatttg tagcgaatac gcacacgtcc ctgacgtaaa tccgtggtgc
     5221 tgttatccac cacgtcatac cagcactccg cgccaatcag tttcccggca gtcaccagtg
     5281 aatccagttt tgccctgatg gcactgataa catccttcac attcgcaggc gtcagtggac
     5341 tgtcgatggt ttcaaactgc gcttccgcaa ttgaatcagc cagcacctgt gcggttcggg
     5401 tatacacctc aaagatgtag gcgttcgttt ccggtgtgcg gttaccccag aagcggaacc
     5461 cgttgcgacg aataatggtc gtgatttctt tgttgttgag gctgttggca tcgctgtctt
     5521 cggcctgcaa cgaccagaac acatgcctcg acatccccag cacattttta accggaacgt
     5581 tggacagcga tttgtgccat ccctgttcat ggtcaatgta cgcacgaagg ccgcacgcat
     5641 aggcaggcgc ggggaacgtt tcgtttttgc catttttcgg gttgtaggcg atgaagtccg
     5701 gccataagag catcacctca cgttcgttga atttctggcg gtaggtaatt gcctcggcca
     5761 tcgtgttaca gccatgacat gaggcataca caaacgcgcg cagtttatct gcaatcacgc
     5821 acagggattt tgttaccgcc tccgtgtcca gctccggcgc ggccagaata cgcggacggt
     5881 atccgatgct ttcatcctgc tctgcaacaa gcagcgcata catccccgta tagctgccgt
     5941 catcctcaga accaccgata accagttgat cctgcgtctt tccgtcttct tctttgtgtt
     6001 cagccacgcg aacgacgatc acctttgtgc tcacctggtc tgcgatggcc ttaagcgcac
     6061 gataaagcgt ccccgttgtc ccgcattttc ccagcacgtc attgacgcgg gtcagcagtg
     6121 tgggcttgtt cagcgggaac agcttcgcgt ccgcatcatc cgccgttgcc acgataccga
     6181 taacgctgga atcaacatcg ttaatcgctg ttaccaggtc ggtattctcc gtaacacggg
     6241 caccatgaaa acgagtttca ctcatagctt cagccccttg tatccgttaa atgattcagc
     6301 aacaatcatc acccaccacg cgcgtaatct cacccctgcg ccgttctccc gccacggcga
     6361 caacaaaaag cagtaacccc ctccgcacgc acatgcgacc atgccgcaca gggagggagc
     6421 agatgaccga caccaccatg caattgctca gtcagggcac ggcccccgtg aaaatgccgg
     6481 attttgatat tctctcggag ggtaaaacgc tgtcaggcgt ggcggagcgc ctgatgagcc
     6541 tgtaactgac cgacaaccgg ggatttgagg cggaccagct caccatcacg ctggatgatg
     6601 cggatggtca gttgcagcta ccgccacggg gcgcgcgtct gacggttctc attggctgga
     6661 aaggggaacc gctgacagaa aaaggcactt acattgttga tgaaatcgct cacgaaggac
     6721 cgccggacag gctgactgtt tcagccagaa gcgcagattt tcgggatgaa tttaacgtta
     6781 aacgtgaggt gtcctggcat gatgtgaccg ttgagcgtgt ggtatccgcc atcactcatc
     6841 ggtacggtct gaaaccgcaa atcagcgaaa tgctgatgga tatcgaaatc gaccacgccg
     6901 accagaccga agaaagcgac atgtcctttc ttacgcgcat ggcggaaatg ctgggagcaa
     6961 tcgccacggt aaaaagcggc aatctgttat tcatcatgcc aggtggtggc gtgaacgcac
     7021 aggggcagcc gttgccctcg ttcgccatta cacgcagcag cggcgatcgc catcagttcc
     7081 gcattgctga ccgcgaggcg tatacggggg tacgcgccta ctggcttgat cttaattacg
     7141 ggaaaaagaa aaaagtcagc gtgaaacgcc gcaaaccgcc aaaacccaaa aaggagaaaa
     7201 gcagcagtcg ggaaggcgac tatatggaag gtgcggaagg caacgtgttt gtgttacgca
     7261 agacttatca gaacgagcag gcagcaagac gcgcagcggc ggcaaagtgg cagcagctac
     7321 aacgcggagc cgcatcattc tccatcacgc tggcgcgtgg tcgtgcagaa ctctatcccg
     7381 aaatgcatgg cacagtgaca ggctttaaaa gcgagattga ttatcaggac tggactattg
     7441 caaaagccga gcacaccatt gataacagcg gctttaccac gcagcttgaa cttgaagcaa
     7501 aaatcccgga atggatagca gaaacagaat aaatctctta aaaatattat ttttgagatg
     7561 tgcttctaca ctgacagtaa gaacaaaaga taatgtatca gcccactaga tgtcagaggt
     7621 tggggcatca accagatgct aacaataata cggatgtttt tcccacatga cagaggaagt
     7681 caagtataat cttcacagtc catcccgtcc aagggaatat gactcaatag taaaagttcc
     7741 agtttagtta gtagacccac actttataga agagtaattc atgattggac aatttttaag
     7801 tgcaacagag attttagcaa agaactatgt tcgtaataaa atggttaaaa atccatttta
     7861 ttcgaattta aaatggaatt ttattgaaaa aaacatcata agattaacat cctcaccggt
     7921 taaatcagta ctttgcatat cagctttttc atttgtacta ctttatgttg gatatttaaa
     7981 cgaattattt ataaaaaata atcttttaca ttatttcccg tttaggcatt cattaaccga
     8041 atggcaaaca accattttaa gtggccaatt gacaattatt ggtattgtat acccccttgt
     8101 tataggttta gtaagtgtct tattccaaaa aaaggcagat agaaaaatag cccaaactgc
     8161 ttatcagcgt tactcaggat tcatgcttgc tggactcagc gggcttttct tatcaggatt
     8221 tatacttctc agtgtactaa ttaaaactgt ctttggaagt tatctttatg gtatagcttg
     8281 tctaatcagc atattatggc ttttgataaa catagttctt tctatttggt tctttatcgt
     8341 gagtcttgaa attcttgatg atgtaaaacg gcaaataatt ataaagcgat acattgcctt
     8401 cgaaatagta atgccccata tttgtaacaa aatatcagcc aagcttaggt tataccctat
     8461 ctatcaaaaa cataactatt caaatttaga aatcacgcaa gccgattaca aaggagaata
     8521 catatctgtg gctagcagtt attctaagga agatgagtta agtttatatc accgtccttt
     8581 tcaacttact cttaatctta ttaattacca actaaaaaag aagaatcatt ttgcgtcatt
     8641 tgtcattggt gataatcgta caaaagaaac agaatccacg ggtaaaatac tttttagcgt
     8701 taaaaatatc aaaccagaca gcttattaat aaaaatactt aagcaatgtt tttatagggc
     8761 tccgattaaa ggaggagatt tttccgtaag tcttacaatg caggctataa cagcagatac
     8821 gtatatgtat ctgcgggatt ctgatctcat tagttttgac aatgctatct ctgcattaat
     8881 taataacttt aataatctct gcgatttgta tttttttcaa gatgataata ccaacaataa
     8941 cttcttactg atcactacag aattatttga acggagtttt caatatgagt tttcagatga
     9001 agtttataaa atatcaaata attccatgga taaaattaat ctttctgaaa gattttttga
     9061 attatgtctt tggagcggag tgcgtattat caataataga aaacatctta tcagcaatga
     9121 actttgtatc tatatgggaa ttactcgttc acaatggtca atattaacag aatggttccg
     9181 aaacaaccaa tcactcttaa atgcctcgct tcgttcacgt tataatcgta tattaagaac
     9241 ctatatcact gtttgggagc aatatcaaga aagcataaac tttcgtttct gcaatacaga
     9301 gaattcagat ttatttgaat tattttgcaa aacgcaacta caagaacttc catcgatgat
     9361 tattgatgca acacagactc gcgatccttc tactattgat acagccgtcg atcttattaa
     9421 ccgatggcag cactcaatga atattgacag ccattcggta gaaaaataca gttacaaagg
     9481 ccagcttttt aacccgggtt ttttcatatc caaaaaacta aacttcaatt cagacagaga
     9541 gtggttcaat attgccatca taaatgcgtt gactgacatg cgtatatgca catgtcttta
     9601 tctgacatca agaatcaata cttctgacaa actaatgaca cattacatta aacttatttt
     9661 agagggtaaa cttattgacc aaaccggtgg atatgaaact cccactgaag aaattgataa
     9721 tgcaagccaa ctaatcaaga tattaattag aatctgcctg tggacatggt ctgaaaatat
     9781 ggagcacaat gggtggatga atagtttagc aaggcgttta cgtgactatg ataaaacaga
     9841 catggtgatg ggcagagttt acagtaatgt ttttgactgt ggatttattg atatggaaca
     9901 atcatgggtt cagctattat taattttttc taacaagaac gatagcgtat caaaagaaat
     9961 aaaagaagca atagaaaaca actatataac atatcgtgag aagcaacgac tcattggagt
    10021 attatcaaaa atttgtaact ccatcgaata cactaaaatc aagctgactt taacattgga
    10081 cgatctccaa acaaaaaaag aaaacttaag aaagttgctt caagagcata ttaacatgct
    10141 aaagaaagat cttgatatga gacttcagga tgctgccata gatgtacatc gactagatag
    10201 cactgcacgt aaaacatcag aacatcttcg taaacgcatc aagaaaacgc tccctctttc
    10261 acttttcaaa tctattgatt ttaagcaggc tagcgattgt ttcacaaaac ataagatatc
    10321 tattaagata gataaagaac cgtatgccga aggaatagaa tccatccctt atatcaatga
    10381 aggtgatatt caagcagact taattctcaa ggacatccag agaataatac tttcaaattt
    10441 atttagcact ggctgttcgc aacataccgt aatagaagat ttcaatatgt tgattgatca
    10501 cataaaatca tctgctgact tggctggcaa actggttctt gtgatgagta aagagatctt
    10561 tcagcaatat aatcgtatgc ttttcgataa tcccaacctc agagaactaa tgagaaaaaa
    10621 tgatgatggc tctatgaaca taactactga atccggtacc cgtaaggttt acttcttacc
    10681 atttgttaat caaccatttt ctcttgtggt aaaggataac tacttcacta aattaattat
    10741 tagagaatat gataataata agttagtaaa tgtcacttca gagaatatca aaagtgactc
    10801 agataaattc aagctaactc ttaactatga gttgaatata gtatttgaag gaaacgctga
    10861 tttaaaaata gctcactcgc aaagagtgac atcagaataa tatctttgac ggcgctcctt
    10921 agagtgccgt tcgtttatta actaatgcta aaaatcatta tatagagaaa gtcatgttta
    10981 aatcttagag aatacaacct agaatagtgt aagcactaca ataagagagg tcgctatgtt
    11041 ccgttgtccg ctttgtggcg catctgcccg tatccgcacc agtcgtccgg aaaatgattc
    11101 aaacaccgtg agacaaaagt attatcagtg taacaatcta gaatgcggcg tatgtttctc
    11161 aacactggaa gccttccaca aattcacttc aaagcacgcc tcccccattc actcatcaga
    11221 gggcatcc
//
LOCUS       DAJTMC010000137        11184 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.137, whole genome shotgun sequence.
ACCESSION   DAJTMC010000137 DAJTMC010000000
VERSION     DAJTMC010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11184
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.137"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(169..375)
                     /locus_tag="OO130_003189"
     CDS             complement(169..375)
                     /locus_tag="OO130_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HCR5366484.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMEQAEKATGDFY"
     gene            687..776
                     /gene="kdpF"
                     /locus_tag="OO130_003190"
     CDS             687..776
                     /gene="kdpF"
                     /locus_tag="OO130_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HCR5366485.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            776..2449
                     /gene="kdpA"
                     /locus_tag="OO130_003191"
     CDS             776..2449
                     /gene="kdpA"
                     /locus_tag="OO130_003191"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HCR5366486.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFAFIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALSADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2472..4520
                     /gene="kdpB"
                     /locus_tag="OO130_003192"
     CDS             2472..4520
                     /gene="kdpB"
                     /locus_tag="OO130_003192"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HCR5366487.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGAMPGNALFSAAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHVEANGGHFPADVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADIAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSITNDVAKYFAIIPAAFAATYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            4529..5072
                     /gene="kdpC"
                     /locus_tag="OO130_003193"
                     /pseudo
     CDS             4529..5072
                     /gene="kdpC"
                     /locus_tag="OO130_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
     gene            5065..7749
                     /gene="kdpD"
                     /locus_tag="OO130_003194"
     CDS             5065..7749
                     /gene="kdpD"
                     /locus_tag="OO130_003194"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HCR5366488.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTDAMLEGLAVLPPKRLAYRGRHICEFDLDAALARR
                     PALILMDELAHSNALGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPGEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSPQDIAATIEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDTIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANALERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHVEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEEFHEDM"
     gene            7746..8423
                     /gene="kdpE"
                     /locus_tag="OO130_003195"
     CDS             7746..8423
                     /gene="kdpE"
                     /locus_tag="OO130_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HCR5366489.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVYEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSAVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFHLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYRFML"
     gene            complement(8639..8896)
                     /locus_tag="OO130_003196"
     CDS             complement(8639..8896)
                     /locus_tag="OO130_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5366490.1"
                     /translation="MQKPLPVRWLIIQGLTENEHCGIQAYACNTESQEDLYAEVPLVG
                     RLTGIEEVKVEAIAMSKLHNMSCPPYMGHSAAVIFHLIYLS"
     gene            9019..>11184
                     /gene="speF"
                     /locus_tag="OO130_003197"
     CDS             9019..>11184
                     /gene="speF"
                     /locus_tag="OO130_003197"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HCR5366491.1"
                     /translation="MSKLKIAVSDSCPDCFTTQRECIYINESRNIDVAAIVLSLNDVT
                     CGKLDEIDATGYGIPVFIATENQERVPAEYLPCISGVFENCESRREFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGEALFRSDLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELIAEVAPQR
                     TKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLDLNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELV
                     DGKPWQSYETAQIAVDLRFFQFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGIDARNG
                     EYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLL
                     ESDAPLAEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQPQKEMFRKEHFPRV
                     SMNPQEANYAYSRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFS
                     ALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDA"
BASE COUNT         2500 a         2842 c         3065 g         2777 t
ORIGIN      
        1 aagtgagata tccatcagat agaaacaatt aggaaaatat ttatatattt ttcctgaata
       61 tatttcagct gggtaatacc atatctaaat atttacctga acatcttcct aataaaaaaa
      121 ccgccagcat ctccactagc ggttttcaat gtattcaaaa ctcggcaatc agtaaaaatc
      181 accggttgcc ttttcggcct gttccatcca aaccggttta tcgctggttt tcgaccagac
      241 gcggtgcagg tagctgtaaa agcgggcgcg gtctttccag aaaagcatga aaggcagcgc
      301 cagaacgccc gctgcaaccg catatgtacg gcgtaaaaag ataagccatg caggatattc
      361 tctgtagagt tccatacttt tctccccctt ttgtatctac ccggtgaata gcaccggaaa
      421 aatgaatttg tttatctgat gaaaatagta ccgccttttg tgtaatttta ctactcatcc
      481 gaccacttat ttttgcttat tgatggttta tttacattca ctttgtaatt aagttgcaca
      541 aaagttaaat taatgctaaa cattagttaa accatggctt ttgccatttt tatacttttt
      601 tacaccccgc ccgcagattt ttgcgaaatc tttgcagcca gaattctact cttccggtat
      661 cacttttagg ccactggagg tgcactgtga gtgcaggcgt gatagccggc gtgttgctgg
      721 tgtttttatt actgggttat ttggtttatg ccctgatcaa tgcggaggcg ttctgatggc
      781 tgcgcaaggg ttcttactga ttgccacgtt tttactggta ttaatggtgc tggcgcgtcc
      841 tttaggcagc gggctggcgc ggctgattaa tgacattcct cttccaggta cagcgggcgt
      901 tgaacgtata ctctttcgtt tacctggcgt ctctgaccat gagatgaact ggaagcaata
      961 tctttgtgcc attctcggcc tgaacatgct ggggctggcg gtgctgtttt ttatgttgct
     1021 cggtcagcac tatctgccgc ttaatccaca gcagttgccg gggctgtcgt gggatctggc
     1081 gctgaatacc gccgtcagct ttgtcacgaa taccaactgg caatcttata gcggtgaaac
     1141 cacgttgagc tatttcagcc agatggcagg cttaacggta caaaactttc tttctgccgc
     1201 cagcgggatt gcggtgattt ttgccttcat ccgtgcgttt actcgccaga gcatgagcac
     1261 actcgggaat gcctgggtcg atctgctacg catcacgtta tgggtgctag tccctgtggc
     1321 gttgttgatc gcactgtttt ttattcaaca aggtgcgctg caaaactttc tgccctatca
     1381 ggcagtgaat accgttgaag gagcgcaaca gctgttaccc atgggaccag tagcttctca
     1441 ggaagcgatc aagatgctcg gtactaacgg cggtggcttc tttaatgcca actcgtcgca
     1501 tccgtttgaa aacccaaccg cactgaccaa cttcgtgcag atgctggcga tcttcttgat
     1561 cccaacagct ctgtgctttg cctttggtga agtgacgggc gatcgccgcc aggggcgcat
     1621 gttgctgtgg gcgatgtcag tgatttttgt catctgcgta ggcgtggtta tgtgggcaga
     1681 agttcagggt aatcctcatc tgctggcgct cagcgcagac agcagcatca atatggaagg
     1741 taaagagagc cgtttcggcg tgctggtcag tagcctgttt gcggtcgtga ctacggcggc
     1801 gtcctgtggc gcggtgattg cgatgcatga ttcgtttacc gctctcggcg gcatggtgcc
     1861 gatgtggcta atgcaaattg gtgaagtggt gttcggcggt gtcggttctg gtctttacgg
     1921 catgatgctg tttgtcctgc tggcggtgtt tattgccggg ctgatgattg gtcgtacacc
     1981 ggaatatctg ggtaaaaaaa tcgacgtacg cgagatgaaa ctgactgcac tggcgattct
     2041 ggttactcca acactggtac tgatgggcgc ggcgttggcg atgatgaccg acgccggacg
     2101 tagcgccatg cttaaccctg gcccgcatgg ttttagcgaa gtgctgtacg ccgtgtcgtc
     2161 cgccgctaac aacaacggca gcgcctttgc cggattaagc gccaactctc cgttctggaa
     2221 ctgtttactg gcgttctgca tgtttgtcgg tcgcttcggg gtgattatcc cggtgatggc
     2281 aattgccggt tcgctggtga gtaaaaagag ccaacccgcc agctccggca cgctgccaac
     2341 gcacggcccg ctgtttgttg gcctgttaat cggcaccgtg ttgctggttg gcgcactgac
     2401 ctttatccct gccctggcgc ttggtccggt ggcggaatat ctctcctgat gatattgagt
     2461 gagcactgaa tatgagtcgt aaacaactgg cgctattcga accaacactt gtcgttcagg
     2521 cgctgaaaga agcggtgaaa aaattaaacc cgcaggcgca atggcgcaat ccggtgatgt
     2581 ttatcgtctg gatcggcagt ctgctgacca cctgtattag catcgcgatg gcaagcggtg
     2641 cgatgcctgg caatgcgctg tttagcgcgg caattagcgg ctggctgtgg gtcaccgtac
     2701 tgttcgctaa tttcgccgag gcgctggcag aaggccgcag taaagcgcag gccaacagtc
     2761 ttaaaggggt gaaaaaaact gcctttgccc gcaagctgcg tgagccgaaa tatggcgctg
     2821 cggcggacaa agttcctgcc gatcaactgc gtaaaggtga catcgtgctg gtagaagctg
     2881 gcgatattat cccctgcgat ggtgaagtca ttgaaggcgg cgcatcggtc gatgaaagcg
     2941 ccatcaccgg ggaatcggca ccggtgatcc gtgaatccgg cggcgatttt gcctccgtca
     3001 ccggcggcac gcgtattctt tctgactggc tggtgattga gtgtagcgtt aaccccggtg
     3061 agacatttct ggatcggatg atcgcgatgg tggaaggcgc acagcgacgc aaaacaccga
     3121 acgagattgc cctgactatc ctgctgattg ctctgaccat cgtcttttta ctggcaaccg
     3181 ccacgctgtg gccgttttcc gcgtggggcg gtaatgccgt gagtgtaacg gtactggtgg
     3241 cgctgctggt ctgtctgatc ccaaccacta ttggcggcct gttgtcagcg atcggcgtcg
     3301 ccgggatgag tcggatgctg ggcgcgaatg tgattgccac cagcggacgt gcagttgaag
     3361 cggcaggtga cgttgacgtt ctgctactgg ataaaaccgg caccatcaca ctcggtaacc
     3421 gtcaggcgtc ggagtttatc cctgcgcagg gcgtggatga aaaaacgctg gctgacgccg
     3481 cacaactggc ttcgctggct gatgaaacgc cggaaggccg cagtattgtg atcctcgcca
     3541 agcaacgttt taacctgcgc gagcgcgatg tgcagtcgct ccatgccacc tttgtaccgt
     3601 ttactgcgca aagccggatg agcgggatca acatcgacaa ccgcatgatc cgtaaaggtt
     3661 ctgtcgatgc cattcgccgc catgttgagg ctaacggcgg tcacttccct gccgatgttg
     3721 atcaaaaagt cgatcaggtt gcgcgtcagg gggccacgcc gctggtggtg gtggaaggtt
     3781 cccgtgtgct gggcgttatt gcgctgaaag atatcgtcaa aggcggtatt aaagagcgct
     3841 tcgcccagct gcgcaaaatg ggcattaaaa cggtgatgat taccggcgat aaccgtctga
     3901 ctgccgccgc cattgctgcg gaagcgggcg tcgatgattt tctcgccgaa gcgacaccgg
     3961 aggccaagct ggcattgatt cgtcagtatc aggcggaagg ccgtttggtg gcgatgaccg
     4021 gcgacggcac caacgatgct ccggcgctgg cgcaagcaga tatcgcggtg gcgatgaact
     4081 ccggcaccca ggcggcgaaa gaggcgggca atatggtcga tctcgactct aacccgacca
     4141 agttgatcga ggtggtgcat attggcaaac agatgctgat gacccgtggc tcgctgacca
     4201 ccttcagcat taccaacgat gtggcgaaat acttcgccat tattccggcg gcgttcgcgg
     4261 caacgtatcc gcagttaaat gcgctgaaca tcatgcgcct gcattcgccc gactccgcca
     4321 ttctcagtgc agtcattttc aacgccttga ttatcgtctt tttgattcct ctggcgttaa
     4381 aaggcgtgag ttataaaccg cttaccgctt ctgccatgtt gcgccgtaac ttatggattt
     4441 acggtctggg tgggctgctg gtgccgttta ttggtatcaa agtcattgat ttactgctga
     4501 ccgtttgcgg tctggtgtga ggtttaccat gcgtggctta cgtccggcat tatcgacatt
     4561 tatctttctg ttattgatta ctggcggcgt ttacccgctg ctgaccaccg tgctggggca
     4621 atggtggttt ccctggcagg ccaacggttc gttgattcgt gaaggtgata cggtgcgggg
     4681 ttcggcatta atcgggcaga attttaccgg caacggcaga aatgccctat aatccacagg
     4741 cttctggcgg gagcaatctg gcggtcagta accctgagct ggataaacaa atagccgcac
     4801 gcgttgctgc attacgggcc gctaacccgg atgccagtac gaacgttccg gttgaactgg
     4861 tgacggcatc ggcaagcggg ctggacaata atatcacccc gcaagcggcg gcctggcaga
     4921 tcccacgcat cgcgaaagcg cgtaatctca gcgttgaaca gctcacgcag ctgatcgcaa
     4981 aatacaacca acaaccgctg gtgaaatata tcggccagcc ggtcgtcaac attgtcgaac
     5041 tcaatctggc gctggataag cttgatgaat aacgaaccct tacgtcccga ccccgatcgt
     5101 ctgctggaac aaactgccgc gccgcatcgg gggaagctga aagttttctt cggtgcctgt
     5161 gcaggcgtcg ggaagacctg ggcgatgctg gcagaagccc agcgactgcg ggcgcaaggg
     5221 ctggatattg tggttggcgt ggtagaaacc cacgggcgaa aagataccga cgcaatgctg
     5281 gaagggctgg ctgttctgcc gccaaaacgc ctggcgtacc gtgggcggca catctgcgag
     5341 tttgacctcg atgccgccct cgcccgccgt ccggcgctga tcttaatgga cgaactggcg
     5401 cacagtaatg cgctaggttc tcgtcaccct aaacgctggc aggacatcga agagctgctg
     5461 gaagcaggca ttgatgtttt cactaccgtc aacgttcagc atctggaaag tctgaatgat
     5521 gtggtcagcg gcgtcaccgg aattcaggta cgggaaaccg tgcccgatcc ttttttcgat
     5581 gccgccgacg acgtggttct ggtggacttg cccccggacg atctgcgcca gcggctgaaa
     5641 gaaggcaaag tctatattgc cgggcaggcg gagcgcgcca ttgaacattt tttccgcaaa
     5701 ggcaatctga tcgccctgcg cgaactggca ctgcgccgta ctgccgatcg cgttgatgag
     5761 caaatgcgcg cctggcgggg gcatcctggc gaagagaaag tctggcacac gcgcgacgcg
     5821 atccttttat gcatcggcca taacaccggt agtgagaagc tggtccgcgc ggcggcgcgg
     5881 ctggcgtcac ggctgggtag cgtctggcac gcggtgtatg ttgaaacccc tgccctgcac
     5941 cgcttaccgg aaaaaaaacg tcgggcaatt ctcagcgcct tacgtctggc gcaggagctg
     6001 ggcgcggaga cggcaacact ttctgatcca gcggaagaaa aagcagtagt gcgttatgcc
     6061 cgtgaacata atctcggcaa gattattctc ggtcgcccgg cctcgcgccg ctggtggcgt
     6121 cgggaaacgt ttgctgaccg actggcgcgc atcgcccccg atctcgatca ggtgctggtc
     6181 gcgcttgatg aaccacccgc ccgcacgatt aacaacgcgc cggatagccg ctcttttaaa
     6241 gacaagtggc gggtacagat tcagggatgc gtggttgccg ccgcgttatg cgccgttatc
     6301 actttaattg ccatgcagtg gctgatggcg tttgatgccg ccaacctggt gatgctgtat
     6361 ctgcttggcg tggtggtggt ggcgctattt tatggacgct ggccttcggt ggttgctacc
     6421 gtcattaatg tggtgagttt cgatctcttt tttatcgccc cacgcggcac gctcgccgtc
     6481 tctgatgtgc aatatctgct gaccttcgca gtgatgttaa ccgtcgggct ggtgatcggg
     6541 aaccttactg ctggcgtgcg ttatcaggcg cgggtagccc gttaccgcga gcaacgcaca
     6601 cggcacttat atgaaatgtc gaaagctctg gcggtgggcc gcagtccgca ggacatcgcc
     6661 gccaccatcg aacaatttat tgcctccacg tttcatgccc gcagtcaggt gttgttgccc
     6721 gatgacaacg gtaaattgca gccgttaaca catccgcaag gaatgacgcc atgggacgat
     6781 accatcgcgc agtggagttt tgataaaggc ctgcctgcgg gcgcgggcac cgacacgtta
     6841 cccggtgtac cgtaccagat tttgccgcta aaaagcggcg agaaaaccta cgggctggtg
     6901 gtggtggagc cggggaatct gcgccagttg atgatcccgg aacagcagcg cctgctggag
     6961 acgtttacgc tgttagtcgc caatgccctt gagcggctga cgctaaccgc cagcgaagaa
     7021 caggcgcgga tggcaagcga acgtgaacag atccgcaacg ccctactggc agcgctttcg
     7081 catgatttac gcacgccgct cacggtgctg ttcggtcagg cagaaatctt aacgctcgat
     7141 ctggcaagcg aaggatcgcc ccacgcccgc caggccagcg agattcgtca gcatgtgctg
     7201 aacactaccc gactggtgaa taatctactg gatatggcgc gaattcagtc cggcggcttt
     7261 aatttgaaga aagagtggtt aacgctggaa gaagtggtcg gtagcgcgct gcaaatgctg
     7321 gaaccgggtt tatcgtcgcc catcaatctt tctctgccag aaccgctgac cttaatccac
     7381 gttgacgggc cactctttga acgggtgctg attaatctgc tggagaacgc ggtgaaatat
     7441 gcgggtgcgc aggccgaaat tggtatcgat gcccacgttg agggcgaaaa tctacaactg
     7501 gatgtctggg ataacggccc cggtcttccg ccaggccagg agcagacgat atttgataag
     7561 tttgctcgcg ggaataaaga gtcggcagta ccgggggtag ggcttggact ggcaatttgt
     7621 cgggcgatag tggatgtaca cgggggcact attaccgcgt tcaaccgacc ggaaggtggt
     7681 gcctgttttc gtgttacact tccccagcaa actgcccctg aacttgaaga atttcatgag
     7741 gatatgtgac aaacgttctg attgttgaag atgaacaggc tattcgtcgc tttctgcgca
     7801 cggcgctgga gggcgacggg atgcgcgtct atgaggccga aacgctgcaa cgcggcttgc
     7861 tggaagcggc tacccgtaag ccagatttga ttattctcga tctcggcctg cccgatggtg
     7921 atgggattga gtttatccgc gacctgcgcc agtggagcgc ggtgccggtg attgtgcttt
     7981 ccgcacgcag cgaagagagc gacaaaatcg ccgcgctgga tgccggagcg gatgattatc
     8041 tgagtaagcc gtttggcatt ggcgaattgc aggcccgtct gcgcgtcgct ttacgccgcc
     8101 actccgctac cgccgcgccc gatccgctgg tgaaattttc cgatgttacc gtcgatttag
     8161 ccgcccgcgt gattcaccgg ggtgaggaag aggtgcatct cacaccaatt gagttccacc
     8221 tgctggcggt gctgctcaac aatgccggaa aagtactcac ccagcgccag ctccttaacc
     8281 aggtgtgggg gccaaacgcg gtcgaacaca gtcactattt gcgtatttat atgggacatc
     8341 tgcgacaaaa actggaacag gatcccgccc gcccacgcca tttcattact gaaaccggta
     8401 ttggctatcg gtttatgctt tgaatattaa ttttaataca gcctgccttt tattaattaa
     8461 agccgtaata ataaatacgg ctttttatct taaacaacac acaaaaataa caattcaata
     8521 ttttatatta ctgagtaaaa agctcatcat taaataaatt aagattgatc attttttatt
     8581 gatcaccttc acagttcaac cgtatttcct ggatagaata tcttcacctt cattcacatc
     8641 aggaaaggta aattaaatgg aaaataacag ccgcactatg ccccatataa ggcggacaac
     8701 tcatattatg aagtttgctc atcgcaatag cttcgacttt cacttcttca atgcccgtta
     8761 gtctaccgac taagggcact tcagcgtaca ggtcttcctg actctctgta ttacaggcat
     8821 atgcctgtat tccgcagtgc tcattctctg ttaacccttg tattatcagc caccggactg
     8881 gtaagggttt ctgcattctg aaaaaagcaa tctacctgat ttatttatca gcggggtaac
     8941 tttgcttttt tccggcacga tcgatttctc attcgagaaa ttgaggacct gctattacct
     9001 aaaataaaga gatgaaaaat gtcaaaatta aaaattgcgg ttagtgattc ttgcccggac
     9061 tgttttacca cgcagcgaga atgtatctac attaatgaaa gtcgtaatat cgatgtggcg
     9121 gcaatcgttt tatcgctcaa cgatgttaca tgcggaaaac tcgatgaaat cgatgccacg
     9181 ggttatggca tcccggtatt tattgctact gaaaatcaag aacgtgtacc cgcagagtat
     9241 ttgccctgta tttcgggtgt ctttgagaat tgcgaatcgc gacgagaatt ttatggtcgt
     9301 cagttagaaa ccgctgccag ccattatgaa actcaactgc gcccaccttt cttccgcgca
     9361 ctggtcgatt atgtcaatca aggtaacagc gcgtttgatt gccctggtca tcagggcggc
     9421 gaatttttcc gtcgccatcc ggcggggaat cagtttgtgg aatactttgg tgaggcgctg
     9481 ttccgttccg acttgtgcaa cgcagacgta gcaatgggtg atctgctgat tcacgaaggc
     9541 gcgccatgca ttgcacagca acatgcggca aaagtgttca atgccgataa aacatacttc
     9601 gttctaaatg gcacttcatc ttctaacaaa gtggttttaa acgccctgct gacaccgggt
     9661 gatctggtgc tgtttgaccg caataaccac aaatctaacc accacggagc gttgctacag
     9721 gctggtgcaa caccggttta tctggaaacc gcacgtaacc cgtatggctt tatcggtggc
     9781 attgatgcgc actgttttga agaaagttac ctgcgtgagc tgatcgcgga agtcgcaccg
     9841 cagcggacaa aagaggctcg tcctttccgc ctcgccgtta ttcagctagg cacctacgac
     9901 ggtacgattt ataacgcccg ccaggtggtg gataaaattg gtcatctgtg tgactacatt
     9961 ctgtttgact ccgcatgggt cggctatgag caatttatcc cgatgatggc tgactgttca
    10021 ccgctgttgc tggatcttaa tgagaacgat ccgggcattc tggttacgca atctgtgcat
    10081 aaacagcagg ctggtttttc tcaaacttca caaatccata aaaaagacag ccacatcaaa
    10141 gggcaacagc gttatgtacc gcacaaacgc atgaacaacg cctttatgat gcatgcctcc
    10201 accagcccgt tctatccgct gtttgccgca ctggatatca acgccaaaat gcatgaaggt
    10261 gtcagcggtc gtaatatgtg gatggattgt gtggtaaatg gcattaatgc ccgcaagctg
    10321 atcctcgata actgtcagca tattcgtccg ttcgtacctg aactagtgga tggtaaaccc
    10381 tggcagtcgt atgaaacagc gcaaattgcg gttgatctgc gcttcttcca gtttgtacca
    10441 ggggaacact ggcactcttt tgaaggctat gcagagaatc aatactttgt cgatccatgc
    10501 aaactgttgc tgacaacccc aggtattgat gcacgtaacg gcgaatatga agcgttcggt
    10561 gtacccgcga cgattcttgc taacttcctg cgcgaaaatg gcgtagtgcc ggaaaaatgc
    10621 gatcttaact ccatcctctt cctgctgact ccggcagaag atatggccaa acttcagcaa
    10681 cttgttgccc tgctggtacg cttcgaaaaa ctgcttgagt ccgacgcgcc attagcagaa
    10741 gtgctgcctt ccatctacaa acagcatgaa gagcgctacg ccggttatac ccttcgtcag
    10801 ttatgccagg aaatgcatga tttgtatgcc cgtcacaacg tgaaacaacc gcaaaaagag
    10861 atgttccgta aggaacactt cccacgcgta agcatgaatc cacaagaagc caactacgcc
    10921 tattcacgcg gtgaagtgga gctggttcgt ctgccggatg ccgaaggccg tatcgctgcc
    10981 gaaggtgcgc ttccttatcc tccgggtgtg ctgtgtgttg ttccgggtga aatctggggt
    11041 ggtgctgttt tgcgttactt cagcgctctg gaagaaggga tcaacctgct gccaggtttt
    11101 gcaccggagc tgcagggtgt ctatatcgaa gaacatgatg gtcgtaagca agtttggtgc
    11161 tatgtcatca agcctcgtga tgcg
//
LOCUS       DAJTMC010000138        11118 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.138, whole genome shotgun sequence.
ACCESSION   DAJTMC010000138 DAJTMC010000000
VERSION     DAJTMC010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11118
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.138"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            1..243
                     /locus_tag="OO130_003198"
                     /pseudo
     CDS             1..243
                     /locus_tag="OO130_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
     gene            414..761
                     /locus_tag="OO130_003199"
     CDS             414..761
                     /locus_tag="OO130_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HCR5366492.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            751..1113
                     /locus_tag="OO130_003200"
     CDS             751..1113
                     /locus_tag="OO130_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HCR5366493.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            1110..1607
                     /locus_tag="OO130_003201"
     CDS             1110..1607
                     /locus_tag="OO130_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HCR5366494.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGGENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(1615..2799)
                     /gene="emrD"
                     /locus_tag="OO130_003202"
     CDS             complement(1615..2799)
                     /gene="emrD"
                     /locus_tag="OO130_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HCR5366495.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCVGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(3218..3307)
                     /gene="tisB"
                     /locus_tag="OO130_003203"
     CDS             complement(3218..3307)
                     /gene="tisB"
                     /locus_tag="OO130_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HCR5366496.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            3872..3970
                     /gene="ivbL"
                     /locus_tag="OO130_003204"
     CDS             3872..3970
                     /gene="ivbL"
                     /locus_tag="OO130_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HCR5366497.1"
                     /translation="MTTSMFNAKLLPTALSAAVVVVRVVVVVGNAP"
     gene            4076..5764
                     /gene="ilvB"
                     /locus_tag="OO130_003205"
     CDS             4076..5764
                     /gene="ilvB"
                     /locus_tag="OO130_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HCR5366498.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIETQPAMAEKAAAPAFSEESIRDAAAMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQDIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            5768..6058
                     /gene="ilvN"
                     /locus_tag="OO130_003206"
     CDS             5768..6058
                     /gene="ilvN"
                     /locus_tag="OO130_003206"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCR5366499.1"
                     /translation="MQNTTHNNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            6132..6722
                     /gene="uhpA"
                     /locus_tag="OO130_003207"
     CDS             6132..6722
                     /gene="uhpA"
                     /locus_tag="OO130_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HCR5366500.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            6722..8224
                     /gene="uhpB"
                     /locus_tag="OO130_003208"
     CDS             6722..8224
                     /gene="uhpB"
                     /locus_tag="OO130_003208"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HCR5366501.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            8234..9553
                     /gene="uhpC"
                     /locus_tag="OO130_003209"
     CDS             8234..9553
                     /gene="uhpC"
                     /locus_tag="OO130_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HCR5366502.1"
                     /translation="MLPFLKAPADAPLMTDKYEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAAAALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            9691..11082
                     /gene="uhpT"
                     /locus_tag="OO130_003210"
     CDS             9691..11082
                     /gene="uhpT"
                     /locus_tag="OO130_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HCR5366503.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
BASE COUNT         2443 a         2893 c         3143 g         2639 t
ORIGIN      
        1 gtggcggtca ggtgcttgct gttccgccag cgttgcgcgt gctgtggtca tacagcgaaa
       61 gaaaatcgcc tgtcacaaag taaattcaga tgccaggtat gtggatatac agcgaacgcc
      121 gatgtaaatg gcgctcgtaa cattttagcg gcggggcacg ccgttcttgc ctgtggagag
      181 atggtgcagt caggccgccc gttgaagcag gaacccaccg aaatgattca ggcgacagcc
      241 tgaacgtagc agggatcctc gtccttcagg gcgtggagga tgtcaatttg cgctatgata
      301 cccatctgac ctccttgtag cggatggcaa aagcatatca ggaaacgggc ggacgaaatt
      361 cttaaaaaaa accgctaaag atagagcgtt agtgaaattg caaaggagca acaatgaaaa
      421 tttcccgcct tggagaagcg cccgattacc gcttctcgct ggctaatgag cgtacttttc
      481 tggcgtggat ccgtaccgca ctaggatttc tggcggcggg ggttgggctt gatcaacttg
      541 cgccagattt cgccacgccc gtcattcgtg aactgctggc gttgttgttg tgtctgtttt
      601 ccggcgggtt agcaatgtat ggctacctgc gctggttacg caatgaaaaa gcgatgcgtc
      661 tgaaagagga cctgccctac accaacagct tattaatcat tagcttaata ttgatggtcg
      721 tggcggtgat cgttatggga ctggtgttgt atgccggata gccgcaaagc cagacgcatt
      781 gccgacccag gactgcagcc ggagcgcaca tcactggcgt ggtttcgtac catgctgggc
      841 tacggcgcgt tgatggcgtt ggctatcaaa cacaactggc accaggcggg catgttattc
      901 tggatttcga ttggcattct cgccatcgtg gcgctgatcc tctggcacta cacccgtaat
      961 cgcaatttaa tggatgtcac gaatagcgat ttttcccaat ttcacgtagt ccgtgacaaa
     1021 tttttgatct ccctcgcggt gttatctctc gcaatactgt ttgctgtaac gcatatacat
     1081 caacttatcg tatttattga gagagtcgca tgacaggaag tcaggtcatt gatgctgaag
     1141 aggacaggca taaactggtt gtagagtaca aggatgctct gcaacctgcc gatttttatc
     1201 acaatttcaa acagcggggc atccgctccg tacagctcat tccctatctt gaatttgatg
     1261 accggggcga tctgacggcc gcttcggtga cagcagagct gtgggggaaa tttttaattg
     1321 ccctgttcga gtgttgggtg cgggcagata tcagtcgtat ctcgattgag ctttttgacg
     1381 ccaccttaca aaaatggtgt ggcggcgaaa acccgcaacc gcgccgtgac tgccaggcgt
     1441 gcgactggca tcgcctgtgc ccgcacgctc ggcaggagac gccagacagc gtgctttgtg
     1501 cgggctatca ggcgttttat tcttactccg caccgcatat gcgggtgatg cgtgatctga
     1561 taaagcagca tcgttctccc atggagctga tgacgatgct gcggtgacgt gcgcttaaac
     1621 gggctgcccc tgatgcgaca tccgcgtcgc cagcggcagc cagcacagca cgatcaacaa
     1681 tcccattaag gtcatcaata accccaggct accctgaccg gtttgcggca acatcgcaga
     1741 gagcgacgcc agcacgccgg aaccaatgtt ttgcagaccg ccgaccagcg cgccagccgt
     1801 gcccgccagg aaggggaacg gctccatcgc gccgctggtc gccagcggaa acagcatccc
     1861 ggcaccgaaa aagaacagcg cggcgggaac gagcagcgtc cagacattca tcacgccaaa
     1921 ccagtcgggg atccacatca gcaagccagc cagcaggcag cagataaccg actgccacat
     1981 taacgtggag aagcgtttat tgggacgtcc ggcaaaccat gcgccaaaaa acgctgccgg
     2041 aatcggcaga ataaacaaaa tactgaccgt catactgctc agccctaaca ccgcgcccat
     2101 cagcacgccg gagcaggctt caaaggcggc aatcccggcc agaccgccaa tcagcatcag
     2161 caaataacag ttaaaaccgc tgttaccgaa aagcgtttta taactggtaa gcaggcgcgt
     2221 gcgcggtgca tcgaccggac gcgtttccgg catccagcgg gccatactga aggtcacacc
     2281 aacacaaaga accaacaaaa agagataaca ggcgcgccag ttccacatcg tatccagcag
     2341 accgccgatt agcggtgcga gcaacggact gacgagaatc cccatgttta acaggctgtt
     2401 agcatggcgc aactgtgtcc gttcatataa atctcgcggc aaagtacgcg ccattacgcc
     2461 gccaacgccg gtgcccatcc cctgcatcgc gctggcggca atcaataccg tcaaactgga
     2521 ggtcgtgacc gcgaccagcg ttgccagcat aaaaatggac attccgacga ggatcaccgg
     2581 tcggcgaccc acacggtcgg aaatcgggcc ataaaacagc tgtgagacac cgtaagtcag
     2641 cagataagcg cccattacgc tctgcaccgc cccttcacgg acgttgagat cgcgcgccat
     2701 atcggcaata gctggaatat aaatggtttg cgccatctga ccgacggcca cgagtaatac
     2761 caacatcaat aacaaattga cgtttctttg ccttttcatt atcacggatg cttttataaa
     2821 aataattaga ataagtcact acgcattccc ataaatgcgg gagaggaatc tatcacattg
     2881 atgaatgtta gccagatata cgccaggaat ggggaattgt ttagcggcag aatatgtaaa
     2941 caaaagcggc aataaatgtt gtcgggaggg attacgtgcg aactgtaacc ctctccccaa
     3001 aggggcgagg gggaaaccac cgttcccttt cagaaaatga gctaaacatt gcacgggacg
     3061 gtcccctcgc ccctttgggg agagggctag ggtgaggggc acaggcagcg caggtgcaaa
     3121 cattctgctg gtagaagaag cccccaactc caacccgcac gctaaatata aaaggggagc
     3181 ggttacccgc tcccctttgg tgcgacttga atctgaatta cttcaggtat ttcagaacag
     3241 catcaagcag ttgcagtgct gcaacaatga gtttgaggat aagaatggcg atatccacca
     3301 ggctcatacg cgtctcctgt ggttcaggag cactgccagc tgacgtacct ttccgctgcc
     3361 tgttgccagc tgttgtgcac gcggtatctc aaggagaaac gttgtgcttg ttgacataac
     3421 acagtgtgct cacgcggggt taaggcgctg acggcaccac ccgtttcagc caggacttag
     3481 tgcgccgggt aacgatgcgg cccccgcata atacattgcg tacagtgata ttatagaagt
     3541 ttactgtata aataaacagt gatatttgga caaaacgcaa actgtgatcg atactcaact
     3601 atgccctaaa tacgtcaaaa ttcgtgccga aattgcgcgt tctgcgcgga acacgtatac
     3661 tttcagtgtt gacataatac agtgtgcttt gcggttacca gctgcaggcg gctgatgaaa
     3721 cctcgctccg gcggggtttt ttgttacctg caattcagta caaaacgtga tcagcccctc
     3781 aattttccct tgctgaaaaa ttttccattg tctcccctgt aaagctgtgc ttgtataaat
     3841 attgttaaac acaaaaccaa caaggtcccc aatgactact tccatgttca acgcaaaact
     3901 actaccaact gcgctatccg ccgcagtggt cgtcgtgcgt gtggtggtgg tcgtcggcaa
     3961 tgcgccgtag ggactggaac aacacacgat tccaaaaccc cgccggcgca aaccgggcgg
     4021 ggtttttcgt ttaagcacct cccggaaagt cggcccagaa gaaaaggact ggagcatggc
     4081 aagttcgggc acaacatcga cgcgtaagcg ctttaccggc gcagaattta tcgttcattt
     4141 cctggaacag cagggcatta agattgtgac gggcattccg ggcggttcta tcctgcctgt
     4201 ttacgatgcc ttaagccaaa gcacgcaaat ccgccatatt ctggcccgtc atgaacaggg
     4261 cgcgggattt atcgctcagg ggatggcgcg caccgacggt aaaccggcgg tctgtatggc
     4321 ctgtagcgga ccgggtgcga ctaacctggt gaccgccatt gccgatgcgc ggctggactc
     4381 catcccgctg atttgcatca ccggtcaggt tcccgcctcg atgatcggca ccgacgcctt
     4441 ccaggaagtg gacacctacg gcatctctat ccccatcacc aaacacaact atctggtcag
     4501 acatatcgaa gaactcccgc aggtcatgag cgatgccttc cgcattgcgc aatcaggccg
     4561 cccaggcccg gtgtggatag acattcctaa ggatgtgcaa acggcagttt ttgagattga
     4621 aacacagccc gctatggcag aaaaagccgc cgcccccgcc tttagtgaag aaagcattcg
     4681 tgacgcagcg gcgatgatta acgctgccaa acgcccggtg ctttatctgg gcggcggtgt
     4741 gatcaatgcg cccgcacggg tgcgtgaact ggcggagaaa gcgcaactgc ctaccaccat
     4801 gactttaatg gcgctgggca tgttgccaaa agcgcatccg ttgtcgctgg gtatgctggg
     4861 gatgcacggc gtgcgcagca ccaactatat tttgcaggag gcggatttgt tgatagtgct
     4921 cggtgcgcgt tttgatgacc gggcaattgg caaaaccgag cagttctgtc cgaatgccaa
     4981 aattattcat gtcgatatcg atcgcgcaga gctgggtaaa atcaagcagc cgcatgtggc
     5041 gattcaggcg gatgttgatg acgtgctggc gcaattgatc ccgcaggtgg aagcgcaacc
     5101 gcgtgcagag tggcaccagt tggtagcgga tttgcagcgc gaattcccgt gtccaatccc
     5161 gaaagcgtgc gatccattaa gccattacgg cctgatcaac gccgttgccg cctgtgtcga
     5221 tgacaatgcg attatcacca ccgatgtggg tcagcatcag atgtggaccg cgcaagctta
     5281 tccgctcaat cgcccacgcc agtggctgac ctccggtggg ctgggcacga tgggttttgg
     5341 cctgcctgcg gcgattggcg cggcgctggc gaacccggat cgcaaagtgt tgtgtttctc
     5401 cggcgacggc agcctgatga tgaatattca ggagatggcg accgccagtg aaaatcagct
     5461 ggatgtcaaa atcattctga tgaacaacga agcgctgggg ctggtgcatc agcaacagag
     5521 tctgttctac gagcaaggcg tttttgccgc cacctatccg ggcaaaatca actttatgca
     5581 gattgccgcc ggattcggcc tcgaaacctg tgatttgaat aacgaagccg atccgcaggc
     5641 tgcattgcag gatatcatca atcgccctgg cccggcgctg atccatgtgc gcattgatgc
     5701 cgaagaaaaa gtttacccga tggtgccgcc aggtgcggcg aatactgaaa tggtggggga
     5761 ataagccatg caaaacacaa ctcataacaa cgtaattctg gagctcaccg ttcgcaacca
     5821 tccgggcgta atgacccacg tttgtggcct ttttgcccgc cgcgctttta acgttgaagg
     5881 cattctttgt ctgccgattc aggacagcga caaaagccat atctggctac tggtcaatga
     5941 cgaccagcgt ctggagcaga tgataagcca aatcgataag ctggaagatg ttgtgaaagt
     6001 gcagcgtaat cagtccgatc cgacgatgtt taacaagatc gcggtgtttt ttcagtaacc
     6061 gctcaaggct tgaacaacat cgcgcttatc gttaaggtaa gcgcgttttt tttacccgcc
     6121 aggacaagac catgatcacc gttgccctta tagacgatca cctcatcgtc cgctccggct
     6181 ttgcgcagct gctggggctg gaacctgatt tgcaggtagt tgccgagttt ggttcggggc
     6241 gcgaggcgct ggcggggctg ccggggcgcg gtgtgcaggt gtgtatttgc gatatctcca
     6301 tgcccgatat ctccggtctg gagctgctaa gccagctgcc gaaaggtatg gcgacgatta
     6361 tgctctccgt tcacgacagt cctgcgctgg ttgagcaggc gcttaacgcg ggggcacgcg
     6421 gctttctttc caaacgctgt agcccggatg aactcattgc tgcggtgcat acggttgcca
     6481 cgggcggctg ttatctgacg ccggatattg ccattaaact ggcatccggt cgtcaggacc
     6541 cgctaaccaa acgtgaacgc caggtggcgg aaaaactggc gcaaggaatg gcggtgaaag
     6601 agattgccgc cgaactgggc ttgtcaccga aaacggtaca cgtccatcgc gccaatctga
     6661 tggaaaaact gggcgtcagt aacgacgtag agctggcgcg ccgcatgttt gatggctggt
     6721 gatgaagacg ttgttctccc gcttaattac cgttattgcc tgctttttta tcttctctgc
     6781 cgcatggttt tgcctgtgga gtatcagcct gcatctggtt gagcgccctg atatggcggt
     6841 gctgttattt ccgtttggtc tgcgtctggg gctaatgctg caatgcccgc gcggatactg
     6901 gccggtgctg ctgggcgcgg agtggctgct gatttactgg ctaacgcagg cggtcggttt
     6961 aacccatttt ccgttattga tgatcggtag tttactgacg ttactgcccg tagcgctgat
     7021 ctcgcgctat cgccatcagc gtgactggcg caccttgctg ttacaggggg cggcgttaac
     7081 ggcggcggcg ttgttgcaat cgctgccctg gctttggcac ggcaaagagt cgtggaatgc
     7141 gctgttgctg actttaactg gcggcctgac gctggccccg atatgtctgg tgttctggca
     7201 ctatctcgcc aataacacct ggctgccgct cggcccgtca ctggtttctc agccaatcaa
     7261 ctggcgcggg cgacatctgg tctggtactt gctgctgttt gttatcagtc tctggctcca
     7321 gttgggattg ccggacgaac tgtcgcgctt tacgccattc tgtctggcgc tgccgattat
     7381 cgcgctggcc tggcactatg gttggcaagg ggcgctgatt gcgacgttga tgaacgccat
     7441 cgcgctgatc gccagtcaaa cctggcgcga tcatccggtg gatttattgc tctcgctgct
     7501 ggtgcaaagt ctgacagggt tgttgcttgg cgctggcatc cagcggttgc gtgaacttaa
     7561 ccagtcgctg caaaaggaac tggcgcgcaa tcagcatctg gctgaacggt tgctggaaac
     7621 cgaagagagc gtgcgccgtg atgtggcgcg tgagctgcat gatgatatcg gtcagaccat
     7681 cactgctatt cgtactcagg cgggcattgt tcagcggctg gcggcagata acgccagcgt
     7741 gaagcagagc gggcagctca tcgaacaact atcgctgggc gtttacgacg cggtgcgccg
     7801 tttgttgggt cggttacgtc cgcgccagtt ggatgatctc accctggagc aggccatccg
     7861 ctcactgatg cgggaaatgg agctggaagg gcgcggtatt gtcagccatc tcgaatggcg
     7921 aatcgatgaa tcagcgttaa gcgaaaacca gcgcgtgacg ctgtttcgtg tctgccagga
     7981 agggctgaac aacattgtga aacatgctga tgccagcgcg gtcaccctgc aaggctggca
     8041 gcaggatgaa cggttgatgc tggttattga agacgatggc agcggtttgc cgccgggttc
     8101 cgggcaacaa ggttttggcc tcaccggaat gcgcgagcgc gtaacggcgc tgggtggcac
     8161 attacacatt tcctgtctgc acggcacgcg tgtcagcgtt tctctacctc aacgctatgt
     8221 ctaaggtttg atgatgttgc cgtttctgaa agcgcctgcc gatgcgccat taatgactga
     8281 taaatatgaa attgatgccc gctatcgcta ctggcgtcgg catattctgc tgaccatctg
     8341 gctgggttac gcgctgtttt acttcacgcg gaaaagtttt aacgccgccg taccagaaat
     8401 ccttgctaac ggcgtgctca gccgtagcga tatcggcctg ttagcgaccc tgttttacat
     8461 tacctatggc gtgtcgaagt ttgtctccgg cattgtcagc gatcgctcaa atgcccgtta
     8521 ttttatgggg atagggctta tcgccacggg cattatcaac attctgtttg gcttctcgac
     8581 gtcgctatgg gcgtttgccg tactctgggt gctgaacgcc tttttccagg gctggggttc
     8641 accggtgtgt gcgcgtctgt taacggcctg gtattcacgt accgagcgcg gtggttggtg
     8701 ggcattatgg aacacggcgc ataacgtcgg cggcgcactc attcccattg tgatggcagc
     8761 ggctgcacta cattacggct ggcgtgccgg gatgatgatt gctggttgta tggcgatcgt
     8821 cgtgggaatt tttctctgct ggcggctgcg cgatcgcccg caggcgttag gtttaccggc
     8881 ggtcggtgaa tggcgacacg acgcgctgga gatagctcaa caacaagaag gggcagggct
     8941 gacgcgtaaa gagatcctca ccaaatatgt gttgctgaat ccgtatatct ggctgctttc
     9001 gttttgctat gtgctggtct atgtggtccg ggcggcgatc aacgactggg gcaatttgta
     9061 tatgtccgag acgctgggcg tcgatctggt cacggcgaat acggcagtga cgatgtttga
     9121 actgggtgga tttatcggtg cgctggtagc gggttggggc tcggacaaat tgtttaacgg
     9181 caaccgaggg ccgatgaatt tgattttcgc cgccggaatt ttgctttcag tcggctccct
     9241 gtggctgatg ccatttgcca gctacgtgat gcaggcaacc tgcttcttca ccattggttt
     9301 ttttgtcttt ggcccacaga tgttaatcgg tatggcggcg gcagagtgtt cccacaaaga
     9361 ggcggcaggg gcggcgacgg ggtttgtcgg cttgtttgct tatctggggg cgtcgcttgc
     9421 tggttggccg ctggcgaaag tactcgatac ctggcactgg agcggatttt ttgtggttat
     9481 ctctatcgcc gccgggattt ccgcactgct gttactgccc tttttgaacg cccagacacc
     9541 gcgcgaagcg tgatgcatct caccttttca cttcatatcc ggcaaaacta agaaattttc
     9601 caggttttgc ctggacgcta tctcaggcct gatttgctgc tgatttttac aatgcatgcc
     9661 tcacgcaggt attcatttca ggagtaaccc atgctggctt tcctaaacca ggttcgcaag
     9721 ccgaccctgg accttccgct cgaagtgcgg cgcaaaatgt ggttcaaacc gttcatgcaa
     9781 tcctacctgg tggtctttat cggctacctg acgatgtacc tgattcgcaa gaactttaac
     9841 atcgcgcaga acgatatgat ttcgacctac gggttgagca tgacgcagct ggggatgatc
     9901 ggcctgggtt tctccatcac ttatggcgtg ggtaaaacgt tggtttcata ctacgccgac
     9961 ggcaaaaaca ccaaacaatt cctgccgttc atgctgatcc tgtctgccat ttgtatgctg
    10021 ggcttcagtg ccagtatggg cagcggctcg gttagcctgt tcctgatgat tgccttctac
    10081 gccttaagcg gctttttcca gagtaccggc ggttcgtgca gttactccac catcaccaaa
    10141 tggacgccgc gtcgtaaacg cgggacattc ctcggtttct ggaatatttc tcacaacctt
    10201 ggcggtgcag gcgcagcagg tgtggcgctg ttcggggcaa attacctgtt cgatggccat
    10261 gtcatcggca tgtttatctt cccgtcgatt atcgcgctga ttgtcggttt tatcggcctg
    10321 cgttacggca gcgactcccc agaatcttat ggcctcggca aagctgaaga actgttcggc
    10381 gaggagatca gcgaagagga caaagagaca gaatctaccg atatgaccaa gtggcagatc
    10441 tttgttgagt atgtgctgaa aaacaaagtg atctggctgc tgtgcttcgc caacattttc
    10501 ctctatgtgg tacgtattgg tatcgaccag tggtcaaccg tatacgcgtt ccaggaactg
    10561 aaactctcta aagcggtggc gattcagggc tttacgctgt ttgaagctgg tgcgctggtc
    10621 ggtacgctgc tgtggggctg gctctctgac ctggcgaacg gtcgccgtgg cctggtggcc
    10681 tgcatcgcgc tggcgctgat tatcgccacg ctcggtgtgt atcaacatgc cagcaacgaa
    10741 tatatctatc tggcttctct ctttgcgttg ggtttcctgg tctttggccc gcaattgttg
    10801 attggtgtgg ctgctgttgg ctttgtacct aaaaaagcga ttggcgctgc cgatggtatt
    10861 aaaggcacct ttgcttacct gattggtgac agctttgcca agttaggtct gggaatgatt
    10921 gccgatggga cgccggtatt cggccttacc ggctgggcag gcaccttcgc cgcacttgat
    10981 atcgccgcga ttggttgtat ctgcctgatg gcgatagtgg cggtgatgga agaacgcaaa
    11041 atccgccgcg agaaaaaaat tcagcagttg acagtggcat aaacgtaact ggtgactttt
    11101 gcccggcatg acgccggg
//
LOCUS       DAJTMC010000139        10950 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.139, whole genome shotgun sequence.
ACCESSION   DAJTMC010000139 DAJTMC010000000
VERSION     DAJTMC010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10950
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.139"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(136..267)
                     /locus_tag="OO130_003211"
                     /pseudo
     CDS             complement(136..267)
                     /locus_tag="OO130_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            549..1907
                     /locus_tag="OO130_003212"
     CDS             549..1907
                     /locus_tag="OO130_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000823236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase-like activity of phytase family
                     protein"
                     /protein_id="HCR5366504.1"
                     /translation="MKRKIIPVLIGCTLSFSALAAQPTAERYVVSFPEGTHVNYAGAF
                     ASAFPNGLPVGIGSGLLFTGKQGDALTFATITDRGPNADSPKEGKNETKIFVTPDFAP
                     LLMTIRVQNGKAEAIDPRPLHDDKGAINGLPLASDVIGSTNEVAFSDTLHRLKGDNRG
                     LDTEGITPDGKGGYWLCDEYGPFLINIDSKGKILAIHGPQAAEGEKAIAGGLPNILKW
                     RQANRGFEGLTRMPDGRIIVAVQSTLDIDAKSKKKALFTRLVSFDPASGKTAMYGYPI
                     DSAAYSKNSDAKIGDIVALDNQHILLIEQGRDKNNRMRNLIYEVDLNKASDLSGFDKP
                     GEYPEFDDEKTLSQRGITLAQKTQVVDLRSLGWQQEKAEGLALIDSKTLAVANDNDFG
                     VKVAMQHPVEGKTFKDYRVNAEGKLTLDDKQVETTLRVKPLEKPESDSELWIVTLPEA
                     LK"
     gene            complement(2146..3531)
                     /locus_tag="OO130_003213"
     CDS             complement(2146..3531)
                     /locus_tag="OO130_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HCR5366505.1"
                     /translation="MNDISSDDIFLLKQRLAEQEALIHALQEKLSNREREIDHLQAQL
                     DKLRRMNFGSRSEKVSRRIAQMEADLNRLQKESDTLTGRVYDPAVQRPLRQTRTRKPF
                     PESLPRDEKRLLPAAPCCPNCGGSLSYLGEDTAEQLELMRSAFRVIRTVREKHACTQC
                     DTIVQAPAPSRPIERGIAGPGLLARVLTSKYAEHTPLYCQSEIYGRQGVELSRSLLSG
                     WVDACCRLLSPLEEALHGYVMTDGKLHADDTPVQVLLPGNKKTKTGRLWAYVRDDRNA
                     GSALAPAVWFAYSPDRKSIHPQTHLACFSGVLQADAYAGFNELYRNGGITEAACWAHA
                     RRKIHDVHVRIPSALTEEALEQIGQLYAIEADIRGMPAEQRLAERQRKTKPLLKSLES
                     WLREKMKTLFFGSGHGGERGALLYSLIGTCKLNDVDPESYLRHVLGVIADWPVNRVSE
                     LLPWRIALPAE"
     gene            complement(3581..3928)
                     /gene="tnpB"
                     /locus_tag="OO130_003214"
     CDS             complement(3581..3928)
                     /gene="tnpB"
                     /locus_tag="OO130_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR5366506.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNSFNGLAAKVQTALKDDPMSGHIF
                     IFRGRSGSQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDW
                     RQPKRLLTSLTML"
     gene            complement(3925..4305)
                     /locus_tag="OO130_003215"
     CDS             complement(3925..4305)
                     /locus_tag="OO130_003215"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HCR5366507.1"
                     /translation="MQKNVTPGRRKGCPNYPPEFKQLLVAASCEPGISISKLALENGI
                     NANLLFKWRQQWREGKLLLPSSESPQLLPVTLDAAAEQPESLAEDPETLSISCEVTFR
                     HGTLRFNGNVSEKLLTLLIQELKR"
     gene            complement(4660..5094)
                     /locus_tag="OO130_003216"
     CDS             complement(4660..5094)
                     /locus_tag="OO130_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HCR5366508.1"
                     /translation="MRILNCYMANDSKGHFVTAKEAAKHNRQDVLCCVSCGCPLTLQR
                     GNDGQPPWFEHDQMTVAEKILLRCTWLDPAEKEARRLHLQGMTVPDYTVKVRKWFCVM
                     CDEDYEGEKCCPRCGTGVYSRAWGRQEVPSEDARADNPLQRL"
     gene            complement(5082..5483)
                     /locus_tag="OO130_003217"
     CDS             complement(5082..5483)
                     /locus_tag="OO130_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5366509.1"
                     /translation="MYAKSFIALDGNGRLTGARTAQAAPYANYTCHLCGSALRYHPQY
                     ETELPWFEHTDDRLTEHGQQCPYVRPERREIQLIKRLQQFVPDALPVVRKASWHCRQC
                     HHDYYGEQYCTHCQTGGFSIPRTTQEEICEF"
     gene            complement(5743..6312)
                     /locus_tag="OO130_003218"
     CDS             complement(5743..6312)
                     /locus_tag="OO130_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HCR5366510.1"
                     /translation="MTYKYNPFWQQRIRETVRHALNVHPRLTALRVDLRLPDVPAATD
                     AAVISRFINTLKARIDAYQKRKHREGKRVHPTTLHYVWAREFGECKGKKHYHLMLLVN
                     RDTWCRAGDYRAPESLAGMIKQAWCSALGVDVGCHATLVHFPAWPAVWLARNDDTGFQ
                     QVLERADYLAKEHTKAHCTGERNFGCSRS"
     gene            complement(7057..7200)
                     /locus_tag="OO130_003219"
                     /pseudo
     CDS             complement(7057..7200)
                     /locus_tag="OO130_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453335.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin activation protein"
     gene            complement(7534..7800)
                     /locus_tag="OO130_003220"
     CDS             complement(7534..7800)
                     /locus_tag="OO130_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5366511.1"
                     /translation="MAEPYRYTGWLLRKDASARTRDKEGIVFVRTGREAGVLIIAELF
                     SEVVTVSQPVPGEEETDITDGIRLSDWYYKYKKSSESNVKDLPE"
     gene            7941..8147
                     /locus_tag="OO130_003221"
     CDS             7941..8147
                     /locus_tag="OO130_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCR5366512.1"
                     /translation="MATPVSLMDDQMVDMAFITQLTGLTDKWFYRLIRDGAFPAPIKL
                     GRSSRWLKSEVEAWLQARIAQPRP"
     gene            8242..8844
                     /locus_tag="OO130_003222"
     CDS             8242..8844
                     /locus_tag="OO130_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5366513.1"
                     /translation="MKQQYQTRYEWLHESYQKWLTGFTRHAVSWGVCHPNIYYFHNLT
                     PGWVSFNGEKPEIAIVPQSLHRLIYGPDKRATPPLDDDLIVNLCTSEHLLVHHPMLEG
                     ILLSECERLRQRSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNSLEDWKENLKRK
                     SEKELEEWIINRQRQSAALTDLMDQYVLLAYRTTVDDSRN"
     gene            10044..>10950
                     /locus_tag="OO130_003223"
     CDS             10044..>10950
                     /locus_tag="OO130_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5366514.1"
                     /translation="MVQKILSDKVMNERTNAYYSYYLGERNISVLPLNVYDPPERFIA
                     YIKKNRENLNITLSDFELEQIISGMRLKALAFLVPLEKISWIAGSERACLFSWYLLMQ
                     FIQNNRAKISADLLQKNKLYLKEEYLEGNAFPSDSSTQFRQILRVLDILSDKNLRDEW
                     IIQTKDRWMRAFKSKSPFSYLLPENEHECIWTWNYLKGKNIALEKLASFPGSADIYHA
                     IHLSFDIWVTCPLTSPDDIKNFRNSFNKAKAQRKYKKMQEDKVNVQFFLDAETRAQLK
                     ELSRVRRLSTGEMLHDLIVEEYKRYR"
BASE COUNT         2634 a         2889 c         2749 g         2678 t
ORIGIN      
        1 gcttctgcta cctgaccatc ataatcacgc agcttcagtg aagcagtgac cacctgttta
       61 acccggtgta tcgttatgat cttatccagc aattgaaccg ccccgggttt cttggagagt
      121 gttttatctg tgaactcagg ctgccagatc atcgtttccg atggaagcat aataagcttt
      181 ttctgcttct gccggtggga tatggccctg cctttccagc aatcgtcgat tgttatacca
      241 gtccacccac gtgagtgtgg tcagctcatc atactgacac gtgcattacc cctaccgcga
      301 taatgcaacc gtacaatata gggcgtttcc taaacgcagc cctcatcact ccaatccaac
      361 aagacacagc atttttgatc acgaagaaag acaaataatt tccgtaaatg tttccgttgt
      421 tacaaataac aacagtgttt tacatttctt tggcctcagg cccgcgcatg ttgccattcg
      481 atgtcatttt cctgactcgt ttcttcttta tggtgtagtg tgatcttcac tttatagatg
      541 ctaacaatat gaaaagaaaa attattcccg tgctaatcgg ttgtacgctc tctttctctg
      601 ccctggcggc acaacctacg gctgagcgct atgtcgtcag ttttccagaa ggcacgcacg
      661 tgaactacgc cggcgcattt gccagtgcat tcccgaacgg ccttccggtt gggataggtt
      721 ctggcctgct gttcaccggt aaacagggcg atgccctgac attcgcaacc attaccgatc
      781 gcggtccgaa cgcggattca ccgaaagagg ggaaaaacga aactaaaatt ttcgttacgc
      841 cagatttcgc gccgctgctg atgacgattc gtgtacaaaa cggcaaagcg gaggccatcg
      901 atccccgtcc tctgcatgac gataaaggcg cgatcaacgg tctgccgctg gcaagcgacg
      961 tgattggctc caccaatgaa gtggccttca gcgacacgtt acatagactg aagggggata
     1021 atcgcgggct ggatacggaa gggatcactc cggatggtaa aggaggttac tggctgtgcg
     1081 atgagtatgg cccgttcctg attaacatcg acagtaaagg gaaaatcctg gcaatccacg
     1141 gtccgcaggc ggcggaaggg gaaaaagcga ttgcgggcgg tttgccaaat atcctcaaat
     1201 ggcgtcaggc gaaccgcggc tttgaaggcc tcacccgtat gccggacggg cgtattattg
     1261 tcgccgtgca aagtacgctg gatatcgacg ctaagagcaa gaaaaaggcg ctgtttacgc
     1321 gtctggtgag cttcgatccg gcaagcggaa aaaccgcgat gtacggttat cctatcgaca
     1381 gcgcggccta cagcaaaaac agcgacgcta aaatcggaga catcgtggcg ctcgataacc
     1441 agcacatcct gctgatcgaa cagggaagag ataaaaacaa cagaatgcgt aacctgattt
     1501 acgaggtcga tctcaataag gcgagcgacc tgtcaggctt cgacaagccg ggtgagtatc
     1561 cggagtttga tgatgagaaa accctgtcac agcgcggcat tacccttgcg cagaaaacgc
     1621 aggttgttga tctgcgctcc ttgggctggc agcaggagaa ggctgaaggg ttggcgctga
     1681 tcgacagcaa aacgctggcg gtggcgaacg ataacgactt tggcgtgaag gtggcgatgc
     1741 aacatccggt cgagggcaag acattcaagg attatcgggt gaacgcggaa ggaaagctga
     1801 cgcttgacga caagcaggtt gaaacgaccc tcagagtgaa gccactggag aaaccggagt
     1861 ctgacagtga actgtggatt gtgacgctgc cggaagcgtt gaaataagtt tataaggtac
     1921 ggacagcagt atcgcttagt ttttcaaatt catgtatgtg aacaatattg gggttccggc
     1981 gtagaccccc aatgaaagcg tcaccacgtg aaaacatgcc tgcacggttt acacgtcatc
     2041 ccatggtggc agccggttaa ctttcctgcg ccaccagtgg aaaaaggcca taagcagtcc
     2101 gaacaaaagt aagcgtacag cgagggccgt attgacgggg atgtgttatt cagctggcag
     2161 tgctatgcgc cacggaagca gttcgctgac ccggttgacc ggccagtctg ctatgacgcc
     2221 aagcacatgg cgaaggtagc tttctggatc cacgtcattc agtttgcacg tcccgatcag
     2281 gctgtacagt agcgctcccc gctcaccacc atggccagag ccgaagaaca gggtcttcat
     2341 cttttcacgc aaccagcttt ccagggattt caacagcggt ttcgtttttc gctgacgttc
     2401 agcaagccgc tgctctgccg gcattcccct tatatccgcc tctatggcgt acaactgacc
     2461 gatctgctcc agggcttctt ccgtcagtgc tgacgggatg cggacgtgca catcgtggat
     2521 ctttcggcgg gcatgagccc agcaggcagc ttccgttatc ccaccattgc gatacagctc
     2581 gttgaacccg gcgtacgcat ccgcttgcag cacaccgctg aagcaagcaa gatgagtctg
     2641 cggatggatg ctttttctgt ccgggctgta agcgaaccac actgcaggtg ccaacgctga
     2701 cccggcattg cggtcatcac gaacatacgc ccacaaccgc ccggtcttcg tcttcttatt
     2761 acccggcagc agtacctgga ccggggtatc atcggcatgg agtttgccgt cagtcatgac
     2821 atagccatga agcgcctctt ccagcggaga cagcagccgg cagcatgcat ccacccagcc
     2881 cgacagcagt gaacggctca gctccacacc ttgccggccg tatatttctg actggcaata
     2941 cagcggggtg tgctctgcat acttcgaggt cagcacgcgg gccagcagcc ccggtccggc
     3001 gataccccgc tcgatgggcc gcgaaggtgc aggtgcctgc acgatggtat cgcactgagt
     3061 acaggcatgt ttttcccgta ccgtccggat aacccggaag gcgctacgca tcaactccag
     3121 ctgttcggcg gtatcctcgc ccagatagct cagtgaaccg ccgcagttcg ggcagcacgg
     3181 cgccgcaggc aacagtcgct tttcgtcacg gggtagtgat tcagggaacg gcttacgggt
     3241 gcgggtctga cgcaacggac gctgtactgc cgggtcatac accctaccag tcagcgtatc
     3301 gctctctttc tgaagccggt tcagatcggc ttccatttgt gcgatacggc gggagacttt
     3361 ttcggaacga ctgccgaagt tcatccggcg gagtttatcc agctgcgcct gcagatggtc
     3421 tatttcgcgc tcccggttgc tcagcttttc ctgcagggcg tggatcagcg cttcctgttc
     3481 ggccaggcgc tgtttcagca ggaagatgtc gtcagaagag atgtcgttca taagcccgta
     3541 ttttaccggg cttattctgt gacaaccagg ataaagagat ttacagcatg gtcagggagg
     3601 tcagcagccg cttgggctgt cgccagtcga taccttccag cagcatcgcc agctgcgcct
     3661 gcgtaaggaa cactttgcca tcacgggctg acggccaggc gaagcgccca cgctccagcc
     3721 gtttggtcag gaggcacagt ccgtcgccgg tggaccacag cagtttaacc tgactgccgc
     3781 tgcggccccg gaaaatgaaa atatggccgg acatgggatc gtctttcagc gccgtttgta
     3841 ctttcgcagc caggccgttg aagctatttc tcatatcggt gataccggca accagccaaa
     3901 ttttggtccc ggaaggtaac gggatcatcg cttcagttcc tgtatcagca gagtcaggag
     3961 cttttcgctg acattgccat tgaagcggag cgtcccgtgc cggaacgtta cctcacagct
     4021 gatactgagg gtttccgggt cctctgcgag cgattctggc tgttcggcag ctgcatcgag
     4081 agtcacagga agtagctggg ggctctctga agaaggtaat agcagctttc cctcgcgcca
     4141 ttgttgtcgc catttgaaca acagattggc gttaatgcca ttttcaagag caagttttga
     4201 gatggatatc ccgggttcac aggaggcagc aacgagcagc tgtttaaatt cgggaggata
     4261 attagggcag ccttttcgcc tgccgggagt cacatttttc tgcatatctg acactttggt
     4321 tcccactact tatttggtgg acaccacttt gtctaattcg tcagattctg accagacggt
     4381 tcaggctgta cgcttaccac tattttatcc ctgttgaatt tggttaagcc ggtgcagacg
     4441 cgccgggaaa cgcacacctg aaccggcaca ataaaataca atacaacccc ctgttactgg
     4501 agatacgaat ggaatgacga ggcagcctgt cagtcacagg gggctgttgg cataaaatca
     4561 acggaaaggc caccaacacc ggaattaagc tccacagtcc ccgcactggc aatccattcc
     4621 cgggaaaggc atcaccgcaa actgctccgg aggcaaccat tacagcctct gtaacggatt
     4681 atcagccctg gcatcttccg acggcacctc ctgccgcccc cacgccctgc tgtatacccc
     4741 ggtaccgcag cgtgggcagc acttttcccc ctcataatct tcgtcacaca tcacacaaaa
     4801 ccactttctc accttcaccg tataatccgg aaccgtcatg ccctgcagat gcaaacgacg
     4861 ggcctctttc tctgccgggt caagccaggt acatcgcagc aggatttttt cagcgacagt
     4921 catctggtca tgttcaaacc acggtggttg tccgtcattg ccccgctgaa gtgttaacgg
     4981 gcatccacag gacacacagc acaaaacgtc ctgtcggttg tgcttcgcag cttctttcgc
     5041 cgtaacaaaa tggcctttgc tgtcattcgc catataacag ttcagaattc gcatatttcc
     5101 tcctgagttg tccggggaat actgaatcct ccggtctggc agtgtgtgca gtactgctcc
     5161 ccataataat cgtggtgaca ttgtctgcag tgccagctgg ctttacgcac cacgggtaag
     5221 gcatccggta cgaattgctg cagacgttta atcaactgta tttctctgcg ctccggcctg
     5281 acataagggc actgttgacc gtgctctgtc agcctgtcgt cagtgtgttc aaaccaggga
     5341 agttcagttt cgtattgcgg atggtatctg agtgcactgc cacacaagtg gcaggtgtag
     5401 ttagcataag gtgcggcctg tgcagtacgg gcgcccgtca gacgtccgtt gccatcaaga
     5461 gcgataaagg attttgcgta catagtcata tacctcagcc gccagacgac acgcaggcaa
     5521 cagccgtccc ggtacgggca gcgtggtcag ggtgtgaatg gcggttcagt taaaatctgg
     5581 actggagttt gggtaaagca ggctggttgc tgttacagca ctgtggttgt cacacaatgg
     5641 aacactgacg taaaaaacag tcagtgggcg agtggataca tcgttgccgc cagacaacaa
     5701 cccatacttt tggtttgccg gatattggcg cgccagtcgt gctcaactcc ggctacagcc
     5761 aaaattgcgc tcaccggtgc agtgagcttt ggtatgctcc ttcgccagat agtcagcacg
     5821 ttccagcact tgctgaaagc cggtgtcatc attacgcgcc agccacaccg ccggccaggc
     5881 cggaaaatgc accagcgtgg catggcaccc gacatccact cccagggcac tgcaccacgc
     5941 ctgtttaatc atcccggcca gagactccgg agcgcggtaa tcaccggcac gacaccaggt
     6001 atcccggttg accagcagca tcaggtgata gtgtttttta cctttgcact ccccaaactc
     6061 ccgggcccag acgtaatgca gggttgtggg atgcacgcgt ttaccttccc gatgcttacg
     6121 tttctggtaa gcgtcgattc gggctttcag ggtattgatg aagcgggata tcacagctgc
     6181 gtccgttgct gccggtacat ccgggagacg caggtcaacc cgcaatgccg tcaggcgggg
     6241 atgaacattc agtgcgtgcc gcaccgtctc acgaatacgt tgctgccaga aggggttgta
     6301 tttgtaggtc atggttaaat ctccgtatgg ttcatacgga atagccacgt cgtaaaaaat
     6361 acgcagagtc cctgaagtga cctccggcag gacactgtct caggcactct gtgataaccc
     6421 gaacggtcgt ctccggactg acggttgaat tttcagcgtc gttttttaat gtaaaaaacc
     6481 ggttacgggt aaggctgtga ggagggagtg atggtgatac gcaaaaagaa gtgcagggac
     6541 tgtggaaacg cgattactca taatacagtg tgttgcccgt attgtggttc tgtcgacccc
     6601 ttcggctact accggaatac agaccgtatc gttaccatac tgctggcgct gattattgtg
     6661 gtcctgctga ctactgtctg cgtcagtgtg tgtatactgt gtagctggtg agataacggt
     6721 tcatgtatcc cctgagctga cagtgtaccg tgtatattaa gcgtaaccga caggttctcc
     6781 ttcataccat attaaatttt ccagggattg ccgctgcctt atttcgtccc atacaggaaa
     6841 actcatctgg gtttctgaca aatacgcgaa actcacagcc cctcaatatc aaccacacgt
     6901 tgtaagccgg taatacctga aaatcagcct attaccgccc acgcctccgg caccattccc
     6961 ttttgatttc gaattctttg ttgtcgctat accgcttaat gttcccggtc ctccggtgca
     7021 gataacaccg gagtcatcgt agcgctgctt caatattcag gtgacgtatt tccagacgga
     7081 agccggcgcg cgatcgtaaa gtttgttcat tgtcagttct gccagccggt tgtcaacggc
     7141 agagttaaag gcttccagct ccgcaagggt aagtttataa cgcgtgtggg aattcacttt
     7201 tcccgcgtct cctgacgtat tacgggctgc cacttacctg gtgtcccgca atacagcctt
     7261 agtgttccgt tatcggggat ccggacgcac cgcaacaacc ggggaagtat ttctggttta
     7321 gagcagtgtc aagagggatt tctcagcccc tcactcaccg gctcagtctc agggcaggag
     7381 cattaatacg ctcagttcat gtcttataca ggaatgggca cagggggagc tggcgtgtca
     7441 ctgctgattt tacgcatcag cgttcatgta tctgcactct gatacatgaa cgattcacag
     7501 cgctctgtta ccggagagat ttactctctt agcttactct ggcaaatcct ttacgttact
     7561 ctctgatgac ttcttatatt tataatacca atcagacagc cttatcccat cggtgatatc
     7621 agtctcctcc tcgccaggaa cgggctgact cactgtaact acctccgaaa acaactctgc
     7681 aattatcaga acgcctgctt ctctccctgt cctcacgaaa actatcccct ctttatcgcg
     7741 cgtgcgtgcg gaagcatctt ttcgcaacaa ccacccggta taccggtacg gctcagccac
     7801 cgcaacctcc ccgtttatct cctggcggtc acattcccga ttatttttta cgtttctccc
     7861 cggttgttat gccggtgaag gtggcgcgct gttttcatga ccacaccggc tggtttaaca
     7921 acatccggag gaacattctc atggctaccc cagtttcact gatggatgac cagatggtcg
     7981 acatggcatt tatcactcaa ctgaccggcc tgacagataa gtggttttac aggctcatca
     8041 gggatggggc ctttccggct cccatcaagc tgggccgcag ctcccgctgg ctgaaaagtg
     8101 aagtggaagc ctggctacag gcgcgtattg cacagccccg cccgtaattt ctgaccgtta
     8161 tccgttcacc cgcagcagac gtaatccccg gcctgccggc ggcattctgc tgcctgttat
     8221 atccccgtga ggaatatgaa aatgaaacaa cagtatcaga cccgctacga atggctccac
     8281 gaaagctacc agaaatggct gaccggcttc acccggcacg ccgtatcctg gggcgtgtgt
     8341 catccgaata tctactactt ccataatctg acgcccggtt gggtgtcatt caatggtgaa
     8401 aaaccggaga ttgccatcgt accccagagc ctgcaccggc tgatttatgg ccctgacaaa
     8461 cgggccacgc cgcccctgga tgatgatctg atagtgaatt tatgcaccag tgaacatctg
     8521 ctggttcatc atccgatgct ggaaggcatt ctcctgtctg aatgtgaacg tctgaggcag
     8581 cgttctctgg caaataaact gatcagcctc ttccgtcagt ttggtggcac ggagttgcgc
     8641 ctcaaactgg tctggctttg ctggcttgat ttaatgaccg gaaacagcct tgaagactgg
     8701 aaagagaacc tgaaacggaa atcagaaaaa gagctggagg aatggatcat caaccgtcag
     8761 aggcagagcg cagcactgac ggatctgatg gatcagtacg tgctcctggc ataccgcaca
     8821 acggttgacg atagccgcaa ctgatgccgg ttgcttccgg aaggtgtgac aaaatacgga
     8881 aatgatgatg ctcccggccc ccatgctcac ctgcagagcg tgttcgtcgt catcatactg
     8941 agccatgacg ggcgtcttaa gggcatcctg tcattgctga gcaggcaaca ccaccagagg
     9001 agccacggac agggatgttc tttacagccc gatacacgcc aggttctcag cagactgaat
     9061 gtgcaggtgc ggttattcac cagaaatggc atgctgcggt acatacccat cctggtgaca
     9121 atatgtacgt ggcgacagcg atcccggcac tgtggaagag actcactcag cctgcaggat
     9181 attctttccg gcatccggga atgtccgcct gaatgcaaac aggatgatat ttgaatccgt
     9241 aatactgttc tttcatcata aataatttat gcagatacag ttaaccgtta cagtagccgg
     9301 tttgttttac cgggaaataa tatccgtcag actgataccg gaacatatct gaaacacgtt
     9361 atgatgtttt gttatcagaa atgttgtata ttcttatccg tggcgacagt ttaatgccct
     9421 ctggtatcac ctgataatgt atatcctgcg atgattcgtt atatttacgg gacacgcctc
     9481 ttttcattgt gctccttttc agacttcatt ttccgtaagt gttccccggc ataaaggagg
     9541 tacctttctg gcgtttataa ctctctttat cggtgacgcc ggtaatacag ggaataccac
     9601 accgacaaac gaggtattaa cagacgatat ttcagtgtcc ataatttttc tgtccggtta
     9661 aataacgtca gtctgctgcc ctctgactgt aatcatcaaa gccggaatgc ataccgggtt
     9721 acaacgtaaa aaaagtgatg ctggcccccg gtgcagctgg cgggcgaaac atgtttacct
     9781 ttcaataccc cctgtctgca gtctccactc ttccatcctc ttaagctacg tcggccacat
     9841 gagtgcactt ccatgataac aggtattcct gtcagcataa cgaagccggt taccttccct
     9901 tcctcctgca tttcctgtca gaatgactat acagcgcgca gattgccttt ttattgacca
     9961 tcttaatata ccgttataaa cttcttttta tcatattatt atcaccttat tatgtttaat
    10021 ttttaagcag tcggagaaag aggatggttc agaaaatttt gtcggataag gttatgaatg
    10081 aaagaacaaa tgcgtattac tcttattatc ttggagagcg taatatctcc gtactgcccc
    10141 ttaatgttta tgatcccccg gaacgtttta tcgcttacat aaaaaaaaac agagaaaatc
    10201 tgaacatcac actttcagat tttgaacttg aacaaataat ttctggtatg cgtctgaagg
    10261 ccctggcctt cctggtcccc ctggaaaaaa tatcctggat cgcgggcagt gaacgcgcat
    10321 gcttattttc ctggtatctg ttgatgcagt ttatccagaa taacagggca aaaataagcg
    10381 cagacttgct tcaaaagaat aaactgtatc ttaaagaaga gtatctggaa gggaatgcct
    10441 tcccgtcgga ctcatcgaca cagttcagac aaatactcag agttctcgat attctttctg
    10501 ataagaattt gcgggatgag tggataatcc agaccaaaga cagatggatg cgggcattta
    10561 aatcaaagag tcctttcagt tacctcctcc cggagaacga acatgaatgc atatggacat
    10621 ggaattattt gaagggaaaa aatattgcac tggaaaaact ggccagtttt cctggttctg
    10681 ctgatattta ccacgcaatt cacctttcat ttgacatatg ggttacctgt ccactcacct
    10741 cacctgacga tataaaaaat ttcagaaaca gtttcaataa ggccaaggcg cagcgcaagt
    10801 acaaaaaaat gcaggaagat aaagtcaatg tacagttttt tcttgacgca gaaaccaggg
    10861 cgcagctcaa agaattatcg agggtcagaa gactcagtac cggcgaaatg ctgcatgacc
    10921 tgattgttga agagtataaa aggtaccgac
//
LOCUS       DAJTMC010000140        10902 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.140, whole genome shotgun sequence.
ACCESSION   DAJTMC010000140 DAJTMC010000000
VERSION     DAJTMC010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10902
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.140"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            <1..184
                     /locus_tag="OO130_003224"
     CDS             <1..184
                     /locus_tag="OO130_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415678.1"
                     /note="interacts RssB preventing the degradation of the
                     stress sigma factor RpoS; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HCR5366515.1"
                     /translation="KLGIILQEKEYPITIYNIAPFNKNLWSLLKSSQECPPGESKITN
                     KCLHNSCIIKICSICY"
     gene            complement(252..1502)
                     /gene="icd"
                     /locus_tag="OO130_003225"
     CDS             complement(252..1502)
                     /gene="icd"
                     /locus_tag="OO130_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HCR5366516.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGEAIIENM"
     gene            1674..2327
                     /gene="rluE"
                     /locus_tag="OO130_003226"
     CDS             1674..2327
                     /gene="rluE"
                     /locus_tag="OO130_003226"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309634.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HCR5366517.1"
                     /translation="MRQFIISENTMQKTSFRNHLVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYTLDNLANGEWREATE"
     gene            2337..2798
                     /gene="nudJ"
                     /locus_tag="OO130_003227"
     CDS             2337..2798
                     /gene="nudJ"
                     /locus_tag="OO130_003227"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HCR5366518.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILKASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            2852..3958
                     /gene="mnmA"
                     /locus_tag="OO130_003228"
     CDS             2852..3958
                     /gene="mnmA"
                     /locus_tag="OO130_003228"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HCR5366519.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            3994..4635
                     /gene="hflD"
                     /locus_tag="OO130_003229"
     CDS             3994..4635
                     /gene="hflD"
                     /locus_tag="OO130_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HCR5366520.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVPAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            4639..6009
                     /gene="purB"
                     /locus_tag="OO130_003230"
     CDS             4639..6009
                     /gene="purB"
                     /locus_tag="OO130_003230"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HCR5366521.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            6177..6848
                     /gene="phoP"
                     /locus_tag="OO130_003231"
     CDS             6177..6848
                     /gene="phoP"
                     /locus_tag="OO130_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HCR5366522.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            6848..8308
                     /gene="phoQ"
                     /locus_tag="OO130_003232"
     CDS             6848..8308
                     /gene="phoQ"
                     /locus_tag="OO130_003232"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HCR5366523.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            8384..9505
                     /gene="roxA"
                     /locus_tag="OO130_003233"
                     /gene_synonym="ycfD"
     CDS             8384..9505
                     /gene="roxA"
                     /locus_tag="OO130_003233"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HCR5366524.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(9554..10780)
                     /gene="pepT"
                     /locus_tag="OO130_003234"
     CDS             complement(9554..10780)
                     /gene="pepT"
                     /locus_tag="OO130_003234"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HCR5366525.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLAGMKGTVERADMH
                     YIIRDFDRKQFEARKCKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRK"
BASE COUNT         2695 a         2787 c         2727 g         2693 t
ORIGIN      
        1 taagcttggt attatccttc aggaaaaaga atatcctatt actatttata acattgcacc
       61 attcaacaag aatttatgga gtttactcaa aagcagtcaa gagtgtcctc caggagaaag
      121 caaaataaca aataaatgtt tacataatag ttgcattata aaaatatgct cgatatgcta
      181 ttaataatat ccacataaat aaaacaacgg gcgtgttata cgcccgtttc aatatttaac
      241 tcatggagag attacatgtt ttcgatgatc gcttcaccaa actctgaaca tttcagcagc
      301 ttagcgcctt ccatcagacg ttcaaagtca taagttacgg ttttcgcgtt gatagcacct
      361 tccatacctt taacaattaa atcagccgct tcagtccacc ccatatggcg cagcatcatc
      421 tcagcggaga ggatgataga gcctggattt actttgtcct gaccggcgta tttcggcgca
      481 gtaccgtggg tggcttcaaa cagggcgcat tcgtcaccga tgtttgctcc aggagcgata
      541 ccgataccgc caacctgcgc tgccagggcg tcagaaatgt agtcaccgtt caggttcata
      601 caggcgataa catcatattc agccggacgc agcaggatct gttgcaggaa tgcatcagca
      661 atcacgtctt taatgacgat ctctttgccg gtgttcgggt ttttaacttt cagccacggg
      721 ccgccgtcga tcagttcacc gccaaactct tcacgcgcca gctggtagcc ccagtcttta
      781 aacgcgcctt cggtgaactt catgatgttg cctttgtgca ccagagtcac agagtcacga
      841 tcgttagcaa ttgcgtattc gatcgctgca cgaaccagac gtttggtgcc ttcttccgaa
      901 cacggcttaa taccgatacc acaatgttcc gggaagcgaa ttttcttcac ccccatctct
      961 tcacgcagga atttaatcac tttctcggcg tcggcagagt ctgctttcca ttcgataccc
     1021 gcataaatgt cttccgagtt ttcacggaag ataaccatat cggtcagttc agggtgttta
     1081 accgggcttg gagtgccctg atagtaacgt accggacgca ggcagatgta gagatccagt
     1141 tcctggcgca gggcaacgtt cagagagcga ataccgccac caacaggagt ggtcagcgga
     1201 cctttaatgg caacgcgata ttcacgaatc agatcaaggg tttcagcagg cagccagaca
     1261 tcctgaccat aaacctgtgt ggatttttca ccggtgtaaa tttccatcca ggagatttta
     1321 cgctcgcctt tataggcttt ctcgactgca gcgtcgacca ctttcagcat ggctggggtt
     1381 acatctacac cgattccatc accttcaatg taagggataa tcggattttc aggaacgttg
     1441 agtttgccgt tttgcagggt gatcttcttg ccttgtgccg gaacaactac tttactttcc
     1501 attcacctct ccttcgagcg ctactggttt gctcgtctgc caccacgttg catatgcgtt
     1561 tgcgtcctgc gatacggatg ctttagagca attttttgtt aatgatttgt aattggcttg
     1621 tcaatactac cctattgttt gccgtcatga aagatgcgcg ttattaggct ataatgcggc
     1681 aattcataat ctctgaaaat accatgcaaa aaacttcttt tagaaatcac ctggttaagc
     1741 gattcagctc gcaacgttct accaggcgta aacctgaaaa ccagcccacg cgtgtgatcc
     1801 tgttcaataa accctacgat gttcttccgc agttcaccga tgaagccgga cgcaaaacat
     1861 taaaagaatt catcccggtt cagggcgttt atgcagcagg tcgtcttgac cgcgatagcg
     1921 aagggttact ggtgctgacc aataacggcg cgttgcaggc gcgtttaacc cagccgggca
     1981 aacgcaccgg gaaaatctat tatgtgcagg tggaaggtat tcccacacaa gacgcacttg
     2041 aagccttgcg caatggcgta accttaaatg atggccctac cctgcccgcc ggcgcggagc
     2101 tggttgacga accagcgtgg ttatggccgc ggaatccacc aattcgtgaa cgcaaaagta
     2161 ttcccaccag ctggctgaag atcaccttat atgaaggacg taatcgccag gtgcgccgca
     2221 tgaccgccca tgttggtttc cccacgctgc gactgattcg ttatgcgatg ggtgattaca
     2281 ctttggataa ccttgccaat ggcgaatggc gagaagcaac agaataagga tgcacaatgt
     2341 ttaaaccaca cgttaccgtt gcttgcgtag tgcacgcaga gggtaaattt ttagtcgttg
     2401 aagagacgat taatggtaaa gcgttatgga accaacctgc cgggcatctg gaagccgatg
     2461 aaaccttagt ggaagctgcc gcccgtgagc tatgggaaga aaccggcatc agcgcgcaac
     2521 cgcaacactt tattcgtatg catcagtgga ttgcgccaga taaaacgccg tttttgcgtt
     2581 tcctctttgc cattgagctt gagcaaatat gcccgaccca gcctcatgac agcgatatcg
     2641 actgctgtcg ttgggtcagc gccgaagaaa ttttaaaggc gtcaaatctt cgttcgccgc
     2701 tggtggcgga aagtatccgt tgttatcaaa gcgggcaacg ctatccgctg gagatgattg
     2761 gcgattttaa ctggcctttt acaaagggtg tcatctaaaa ggctgcgtga tagaatacgc
     2821 cgccttgaag ttcaatgtcg tgagtgatcc aatgtctgaa accgcaaaaa aagtaatcgt
     2881 cggcatgtcc ggcggtgtcg attcctccgt ttctgcctgg ctgttgcaac aacagggata
     2941 tcaggttgaa ggcctgttta tgaagaactg ggaagaagac gacggtgagg aatattgcac
     3001 agcggcagcg gatctggctg atgcccaagc tgtctgcgac aagctcggca ttgaactgca
     3061 caccgttaac tttgctgccg agtactggga caacgtcttc gaactgttcc ttgccgaata
     3121 taaagccggt cgcacgccga atccggatat tctgtgcaac aaagagatca aatttaaagc
     3181 tttcctcgaa tttgccgccg aagatttagg tgccgattat atcgctaccg gtcattacgt
     3241 acgtcgggcc gatgtcgatg gcaagagccg cctgctgcgt ggtctggaca gcaataaaga
     3301 ccagagctac ttcctttata cgctcagcca tgagcagatt gcgcaaagcc tgttcccggt
     3361 cggcgaactg gaaaaaccgc aggtgcgtaa gattgctgaa gatcttggtc tggtcaccgc
     3421 gaagaaaaaa gactctaccg gcatttgctt cattggcgaa cgtaaattcc gcgagttcct
     3481 gggccgttat ctcccggcgc aaccgggcaa aatcattacc gtcgatggcg atgaaattgg
     3541 tgagcaccag gggctgatgt atcacactct cggtcagcgt aaaggtctgg gtatcggtgg
     3601 caccaaagaa ggcaccgaag aaccgtggta tgtggtggac aaagacgtcg aaaacaacat
     3661 tctggttgtt gcgcagggcc atgaacaccc gcggctgatg tctgtcgggt tgattgccca
     3721 gcagttgcac tgggtcgatc gcgaaccatt caccggtact atgcgttgca cggtaaaaac
     3781 ccgctatcgc cagaccgata tcccttgcac cgtcaaggcg ctggacgatg atcgcattga
     3841 agtgattttc gatgaaccgg ttgccgccgt gacgccgggc cagtctgccg tcttctataa
     3901 cggtgaagtg tgcctcggtg gcggtattat tgagcagcgt ctgccgctgc cggtctgatt
     3961 attatcttta cttaaaacaa ggaagcagtg aacgtggcaa agaattacta tgacatcacc
     4021 ctcgccctgg ccggtatttg tcagtcggca cgcctggtgc aacaactcgc tcaccagggg
     4081 cattgtgatg ccgatgcgct acacgtctca ctcaacagta ttattgatat gaaccccagc
     4141 tcgacgctgg cggtttttgg cggtagcgaa gccaacctgc gcgtcgggct ggaaaccctg
     4201 ctcggcgtgc tcaatgccag cagtcgccag ggcttaaacg ccgaattaac ccgctacaca
     4261 ctcagcttga tggtgcttga gcgcaaactc tcctcagcga aaggcgcgct cgacactctg
     4321 ggcaaccgga tcaacggcct gcaacgccag ctcgaacact tcgatttaca gtccgaaacg
     4381 ctgatgagcg cgatggctgc tatctatgtt gatgtgatca gcccgcttgg cccgcgcatt
     4441 caggtcaccg gttcccctgc tgtactgcaa agcccacaag tgccggcgaa agttcgcgca
     4501 accctgctgg caggcattcg cgccgccgtg ctctggcacc aggtcggcgg cggacgtctg
     4561 caactgatgt tttctcgtaa tcgcctgacc actcaggcaa aacaaattct tgctcattta
     4621 accccggagt tgtgatctat ggaattatcc tcactgaccg ccgtttcccc tgtcgatgga
     4681 cgctacggcg ataaagtcag cgcgctgcgc gggattttca gcgaatatgg tttgctgaaa
     4741 ttccgtgtac aagttgaagt acgttggctg caaaaactgg ccgcgcacgc agcgatcaag
     4801 gaagttcctg cttttgctgc cgacgcaatc ggttaccttg atgcaattgt cgccaatttc
     4861 agcgaagaag atgccgcacg catcaaaacc atcgagcgta ccactaacca cgacgttaaa
     4921 gcggttgagt atttcctgaa agaaaaagtg gcggagatcc cggaactgca cgcggtttct
     4981 gaattcatcc actttgcctg tacttcggaa gatatcaata acctctccca cgcattaatg
     5041 ctgaaaaccg cgcgtgatga agtgatcctg ccgtactggc gtcaactgat tgatggcatt
     5101 aaagatctcg ccgttcagta ccgcgatatc ccgctgctgt ctcgtaccca cggtcagcca
     5161 gccacgccgt caaccatcgg taaagagatg gcaaacgtcg cctaccgtat ggagcgccag
     5221 taccgccagc ttaaccaggt ggagatcctc ggcaaaatca acggcgcggt cggtaactat
     5281 aacgcccaca tcgccgctta cccggaagtt gactggcatc agttcagcga agagttcgtc
     5341 acctcgctgg gtattcagtg gaacccgtac actacccaga ttgaaccgca cgactacatt
     5401 gccgaactgt ttgattgcgt tgcgcgcttc aacaccattc tgatcgactt tgaccgtgac
     5461 gtctggggtt atatcgccct taaccacttc aaacagaaaa ccattgctgg tgagattggt
     5521 tcttccacca tgccgcataa agttaacccg atcgacttcg agaactccga agggaatctg
     5581 ggcctttcca acgcggtatt acagcatctg gcaagcaaac tgccggtttc ccgctggcag
     5641 cgtgacctga ccgactccac cgtgctgcgt aacctcggcg tgggtatcgg ttatgcgctg
     5701 attgcgtatc aatccaccct gaaaggcgtg agcaaactgg aagtgaaccg tgaccatctg
     5761 ctggatgagc tggatcacaa ctgggaagtg ctggctgagc caatccagac agttatgcgt
     5821 cgctatggca tcgaaaaacc gtacgagaag ctgaaagagc tgactcgtgg taagcgcgtt
     5881 gacgccgaag gcatgaagca gtttatcgac ggtctggcgt tgccagaaga agagaaagcc
     5941 cgcctgaaag cgatgacgcc ggcaaactac attggtcgcg ccatcaccat ggttgatgag
     6001 ctgaaataaa ctcgtatcag tgccggatgg cgatgctgtc cggcctgctt attaagatta
     6061 tccgcttttt attttttcac tttacctccc ctccccgctg gtttatttaa tgtttacccc
     6121 cataaccaca taatcgcgtt acactatttt aataattaag acagggagaa ataaaaatgc
     6181 gcgtactggt tgttgaagac aatgcgttgt tacgtcacca ccttaaagtt cagattcagg
     6241 atgctggtca tcaggtcgat gatgcagaag atgccaaaga agccgattat tatctcaatg
     6301 aacatttacc ggatattgcg attgtcgatc tcggattgcc agacgaggac ggtctgtcac
     6361 tgattcgccg ctggcgtagc aatgatgttt cactgccgat tctggtatta accgcccgtg
     6421 aaagctggca ggacaaagtc gaagtattaa gtgctggtgc tgatgattac gtgactaaac
     6481 catttcatat tgaagaggtg atggcgcgaa tgcaggcatt aatgcggcgt aatagcggtc
     6541 tggcttcaca ggtcatttcg ctacctccgt ttcaggttga tctctctcgc cgtgaattat
     6601 ctattaatga cgaagtgatc aaactgaccg cgttcgaata caccattatg gaaacgttga
     6661 tacgcaataa tggcaaagtg gtgagcaaag attcgttaat gctccaactc tatccggatg
     6721 cggagctgcg ggaaagccat accattgatg tactgatggg acgtctgcgc aaaaaaattc
     6781 aggcacaata tccgcaagaa gtgattacta ccgttcgcgg ccagggctat ttgttcgaat
     6841 tgcgctgatg aaaaaattac tgcgtctttt tttcccgctc tcgctgcggg tacgttttct
     6901 gttggcaacg gctgcggtag tactggtgct ttcgcttgcc tacggaatgg tcgcgctgat
     6961 cggttatagc gtcagtttcg ataaaactac gtttcggctg ttacgtggcg agagcaatct
     7021 gttctatacc cttgcgaagt gggaaaacaa taagttgcat gtcgagttac ccgaaaatat
     7081 cgacaagcaa agccccacca tgacgctaat ttatgatgag aacgggcagc ttttatgggc
     7141 gcaacgtgac gtgccctggc tgatgaagat gatccagcct gactggctga aatcgaatgg
     7201 ttttcatgaa attgaagcgg atgttaacga taccagcctc ttgctgagtg gagatcattc
     7261 gatacagcaa cagttgcagg aagtgcggga agatgatgac gacgcggaga tgacccactc
     7321 ggtggcagta aacgtctacc cggcaacatc gcggatgcca aaattaacca ttgtggtggt
     7381 ggataccatt ccggtggagc taaaaagttc ctatatggtc tggagctggt ttatctatgt
     7441 gctctcagcc aatctgctgt tagtgatccc gctgctgtgg gtcgccgcct ggtggagttt
     7501 acgccccatc gaagccctgg caaaagaagt ccgcgaactg gaagaacata accgcgaatt
     7561 gctcaatcca gccacaacgc gagaactgac cagtctggta cgaaacctga accgattgtt
     7621 aaaaagtgaa cgcgaacgtt acgacaaata ccgtacgacg ctcaccgacc tgacccatag
     7681 tctgaaaacg ccactggcgg tgctgcaaag tacgctgcgt tctctgcgta gtgaaaagat
     7741 gagcgtcagt gatgctgagc cggtaatgct ggagcaaatc agccgcattt cacagcaaat
     7801 tggctactac ctgcatcgtg ccagtatgcg cggcgggaca ttgctcagcc gcgagctgca
     7861 tccggtcgcc ccactgctgg acaatctcac ctcagcgctg aacaaagtgt atcaacgcaa
     7921 aggggtcaat atctctctcg atatttcgcc agagatcagc tttgtcggtg agcagaacga
     7981 ttttgtcgag gtgatgggca acgtgctgga taatgcctgt aaatattgcc tcgagtttgt
     8041 cgaaatttct gcaaggcaaa ccgacgagca tctctatatt gtggtcgagg atgatggccc
     8101 cggtattcca ttaagcaagc gagaggtcat tttcgaccgt ggtcaacggg ttgatacttt
     8161 acgccctggg caaggtgtag ggctggcggt agcccgcgaa atcaccgagc aatatgaggg
     8221 taaaatcgtc gccggagaga gcatgctggg cggtgcgcgg atggaggtga tttttggtcg
     8281 ccagcattct gcgccgaaag atgaataaat atgtccatac ttcacgcatt acgttaagca
     8341 tccgttataa tcggttgcag ataccagcct gtggatgctt aacatggaat accaactcac
     8401 tcttaactgg cccgattttc ttgaacgtca ctggcagaaa cgcccggtgg tgttaaaacg
     8461 cggctttaat aattttattg acccgatctc tccagacgag ttggcgggtc tggcgatgga
     8521 aagcgaagtt gacagtcgac tggtcagtca ccaggatggc aaatggcagg tcagccacgg
     8581 cccgttcgaa agctacgatc atctcggtga aaccaactgg tcattactgg tacaggcagt
     8641 gaaccactgg catgagccga ccgccgcgct gatgcgaccg ttccgtgaac taccggactg
     8701 gcgtattgat gatctgatga tttctttttc tgtacctggc ggcggcgtcg gcccgcatct
     8761 cgatcagtac gacgtgttta tcattcaggg taccggacgt cgtcgctggc gagtgggcga
     8821 aaagctgcaa atgaaacagc actgcccaca tccggatctg ttacaggtcg atccgttcga
     8881 agccatcatc gatgaagagc tggagcctgg tgatattctt tatattccgc caggattccc
     8941 gcatgaaggc tacgcgctgg aaaatgcgat gaactattcc gtgggctttc gcgcgccaaa
     9001 tacgcgggaa ctgattagtg gatttgccga ttatgtgctg caacgtgaac tgggcggcaa
     9061 ctactacagc gatcctgatg ttccacctcg cgctcatcct gcggatgttc tgcctcaaga
     9121 gatggataaa ctgcgtgaga tgatgctcga attgatcaac cagccggaac actttaagca
     9181 atggtttggc gagtttatat cccagtcacg tcatgaactg gatatcgcgc cgccggaacc
     9241 gccttatcag ccagatgaaa tctacgatgc gctgaaacaa ggtgatgtgc tggtgcgcct
     9301 gggtggtctg cgcgtattgc gcattggcga cgacgtgtat gccaatggtg agaagatcga
     9361 ttccccgcac cgtccggcac tggatgcact cgccagcaac attgcgctga ctgcggagaa
     9421 ttttggcgat gcgctggaag atccgtcatt cctcgcgatg ctcgcggcgc tggtcaatag
     9481 cgggtattgg ttcttcgaag ggtaagtttg gatttaagcc ggatgcggca tcacatgccg
     9541 catccctttt cgcttacttc cgttgcgccg ttaactcggc aatacggacg atcacctgca
     9601 ccgctttttc cataccttcc agagtcacaa actcatgctt accatgatag ttgtaaccgc
     9661 cagtgaacaa gttcgggcac ggtaatccca taaacgacaa ctgcgcgccg tcggtaccgc
     9721 cgcggatcgg tttcagttcc ggttcaatat cgcagtcacg cattgcctgc tgggcgatat
     9781 cgagaatatg cggatgctca accactttct cgcgcatatt gtagtaactg tcttcaatca
     9841 ccagttcaat gtagcaatca ggatgtaacc ctttgcccac ttttttggcg atctccatca
     9901 ttttacattt acgcgcttca aactgtttac ggtcgaaatc acggatgatg tagtgcatat
     9961 cggcccgttc aacggtgcct ttcatgcccg ccaggtgata gaaaccttca tagccttctg
    10021 tcatttccgg gctttcatcc gccggaactt ccgcatgaat acgtgccgcc agcgacagcg
    10081 catttaccat cactcctttc gccgtgcccg gatgaacatt gttaccgaca attttgatat
    10141 tgaccgatgc ggcgttgaag ttttcaaact ccagttcgcc tacgccacca ccgtcaacag
    10201 tgtaagccca gcgggcatcg aaggcatcaa catcaaaatg tttcgcccct ttgcccactt
    10261 cttcatccgg ggtaaaggcg acgcgaatat caccatgcgg aatgtttttc tgttgcaata
    10321 ccgccagcgc ggtcatgatt tctgcaatac ctgctttgtc atcggcacct aacaaggttt
    10381 taccatcggt ggtaatcaat gtctgaccca gtagctgatg cagcaccggg aacataaccg
    10441 gtgataaaac ttcatcgccg atacccagcg caatatcgcc accgcgatag ttttcaacaa
    10501 tttgcggatt aacatttttg ccgctgcaat ccggtgaggt atccacatga gaaataaagc
    10561 caatcgccgg gatatcgcca gggacgttag ccggtaacgt cgccatcaaa gtgcccttct
    10621 cacttaaggt cacattgata agccccattt cttcgagctg ctctttcagc agatgcaata
    10681 acttccattg gccttccgtg ctgggaacct gtctcacccc tgcttttgat tgggtatcca
    10741 gagacacgta gttcaaaaat cgctcaagta gtttatccat gtagtcaccc tcactttttg
    10801 tgacaacatt attaagaagc aagaaaagac aaatattgcg tcaggtcact tttacccctg
    10861 caagcgggaa tatttatcag cattacctta atgaatataa ag
//
LOCUS       DAJTMC010000141        10897 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.141, whole genome shotgun sequence.
ACCESSION   DAJTMC010000141 DAJTMC010000000
VERSION     DAJTMC010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10897
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.141"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(228..2087)
                     /gene="gss"
                     /locus_tag="OO130_003235"
     CDS             complement(228..2087)
                     /gene="gss"
                     /locus_tag="OO130_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HCR5366526.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            2292..3158
                     /gene="yghU"
                     /locus_tag="OO130_003236"
     CDS             2292..3158
                     /gene="yghU"
                     /locus_tag="OO130_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HCR5366527.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPICVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(3281..3529)
                     /gene="hybG"
                     /locus_tag="OO130_003237"
     CDS             complement(3281..3529)
                     /gene="hybG"
                     /locus_tag="OO130_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HCR5366528.1"
                     /translation="MCIGVPGQVLAVGEDIHLLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(3542..3883)
                     /gene="hypA"
                     /locus_tag="OO130_003238"
     CDS             complement(3542..3883)
                     /gene="hypA"
                     /locus_tag="OO130_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCR5366529.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(3876..4364)
                     /gene="hybE"
                     /locus_tag="OO130_003239"
     CDS             complement(3876..4364)
                     /gene="hybE"
                     /locus_tag="OO130_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HCR5366530.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(4357..4851)
                     /locus_tag="OO130_003240"
     CDS             complement(4357..4851)
                     /locus_tag="OO130_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HCR5366531.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(4851..6554)
                     /gene="hybC"
                     /locus_tag="OO130_003241"
     CDS             complement(4851..6554)
                     /gene="hybC"
                     /locus_tag="OO130_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HCR5366532.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(6551..7729)
                     /gene="hybB"
                     /locus_tag="OO130_003242"
     CDS             complement(6551..7729)
                     /gene="hybB"
                     /locus_tag="OO130_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HCR5366533.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLKRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTREELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(7719..8705)
                     /gene="hybA"
                     /locus_tag="OO130_003243"
     CDS             complement(7719..8705)
                     /gene="hybA"
                     /locus_tag="OO130_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HCR5366534.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(8708..9826)
                     /gene="hybO"
                     /locus_tag="OO130_003244"
     CDS             complement(8708..9826)
                     /gene="hybO"
                     /locus_tag="OO130_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HCR5366535.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(10015..10302)
                     /locus_tag="OO130_003245"
     CDS             complement(10015..10302)
                     /locus_tag="OO130_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HCR5366536.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(10421..10897)
                     /locus_tag="OO130_003246"
                     /pseudo
     CDS             complement(10421..10897)
                     /locus_tag="OO130_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
BASE COUNT         2660 a         2990 c         2692 g         2555 t
ORIGIN      
        1 caaagtgaaa tatcattttt taacgataaa aagagtgtta tttcttgtgt atcagacaat
       61 aagcattcat tcaaaacctt tagaaataat caaaacacag gtattccact ggttaatata
      121 ttcacatatg aaatgaataa atattagaac atataaatat tgaatatttt gattaatccc
      181 cgtactgatt attcttcata atcagtacgg ggttgctaca acatgtatta ctttttcacc
      241 acaattaacg gttcaatatc actctctttt ttgatgacca gtgattcatc accgcgcaaa
      301 cacgtcccac cgtagttgcc gcccacggtg aaggtacata cctgaatgta tttaccgtcc
      361 actttcggca aacaccacag ttgctgatag atgtttttct gctcggcaaa tttaccgctg
      421 gttttgtcca gcacctcttc atgatggctg accaggtcga tattgctacc acagcgaccg
      481 gcaatcggtt tcactgcgta acctgttttc accaattcat cattaacagt gaaatcggta
      541 tccagcaggt aacgatggtg cgggaacagc gaccagagga tcggcagaat cgctttgttg
      601 ccggggatca ccgtccacag cggctcaaag actagcactt ccgggcgcag caatacgtcg
      661 ataagacgca cttcgttttg cggatgaccg gtacggattg gcaccgcagc aaactcacgg
      721 tcgctaactt cacgaatctg atcaaacgcg gtttcccacg cccaggtttt ccacacgcag
      781 ttaaccagtc gcccttcccc atcaatcagt tgcccggcag catcccagcc cagttcatcc
      841 aatccacgca agatacgcgt ttcaaagccc gcctggtgca gcgcctgctc cataaactgc
      901 gcgtgatagt tttcctcgat atctttgtcc tgcatgatat ggacaaacgg acgtgcacga
      961 ctgtgtttcc aggcaccagc caattcgtta atcagccctt ccgccggatt gaagccgttg
     1021 cctttatagc cctgctccgc ccaacgttcg aggatcaagc ccgcttcggt atgacaggag
     1081 gcggagtcgg cgttgtactc gtaaaccttc aggccacgct catccatgca gaaatccata
     1141 cgaccagtga tcatatggtg acggcgacgc tgccaggaga gacgcaaacg tggccagagg
     1201 attttcggaa tgtcgaacag cgccagcagg ttgtcatctt tcagtacctt gtcggttgcg
     1261 tgaagataca tcaggtgcag ctcgttggtg gctttaatta gctcctgctc ggcactctcg
     1321 gtaatggtgt agtaatgata aggatcctga ttgatcacct gaccgttggc ctgcacatag
     1381 gcgttttgca gcggatcttt ttcatccagc catttaccgt caaactggcc tttgttttcc
     1441 aggcgcgctc cgctgatttt cagcagctcg cctgcaattt ccggctgcgg taagctgtat
     1501 tcagtatctt ccgtctggat catccagccc agaatggtgg tgtcatcaaa agtgtctttc
     1561 agggtatagc agccgttttc gaccaccatc tccagctcac gcgtccactg ttgcccttgc
     1621 ggcaacgggg aatgaatcac gttctgttcc gcaatacgga ctttgttgcc atgcaattgg
     1681 gtaatgatgg cgacatggcc agtgtcttta aattcaccgc ctttatccca gataagaagc
     1741 gcacccgcga ccggcgcacg cggcgagccg ttaggaaatg cctgcaatgg caggatgttg
     1801 tcattaacca cttctcgcag gaagcgcagc gagaaaatct cccacgccat acccacgtca
     1861 gtaaagacca caccgtaatt cagaaagaga aaacggcgag caaattcaac gcattgccac
     1921 ttgtggccca tatattcgtc gtcgatatag ctacggaata cggcgtcatc ttcgtattcc
     1981 tgcggatcga gagaactgta atctgaagag tagattgcta ccccacctgg ggcgtagccc
     2041 aataatgtcc cgaacggggc atcctggctg gtcgttcctt tgctcatcac tttaccttta
     2101 acaatacaac ctaagcagtt ggcgtaattg ttgtgctctg attacctgct cagcgacatt
     2161 aaccggacag aggtcagact aatcatacac ctcatcgcag cggctggcgc aaagggttaa
     2221 aaaattcaca ttctgtacag caagtgacct gccacactgc ttcaacacta ccactcagaa
     2281 ggcaactcac tatgacagac aatacttatc agcccgcgaa agtctggacg tgggataaat
     2341 ccgctggcgg cgcgttcgcc aatatcaatc gcccggtttc tggtccgacg catgaaaaaa
     2401 cgctgcccgt tggcaaacac ccattgcaac tttattcgct gggaacgccg aacggtcaga
     2461 aagtaacgat tatgcttgag gagctgctgg cgctgggcgt tactggtgca gagtacgacg
     2521 cctggctgat tcgtattggc gatggcgatc aattctccag cggctttgtc gaagtgaacc
     2581 caaactcgaa gatcccggcg ctgcgcgatc atacgcataa tccgccgatc tgtgtgtttg
     2641 aatctggttc gatcctgctt tatctggcgg agaaatttgg ctacttcctg ccgcaggatt
     2701 tggcaaagcg tactgaaacg atgaactggc tgttctggtt acagggcgcg gcaccgttcc
     2761 tcggcggtgg ttttggtcac ttttaccatt acgcaccggt aaagattgag tacgccatca
     2821 accgctttac catggaagcc aaacgtctgc tcgacgtgct ggataagcaa ctggcgcagc
     2881 ataagtttgt tgcgggcgat gagtacacca ttgcggatat ggcgatttgg ccgtggtttg
     2941 gcaacgtggt gttaggtggt gtgtatgatg ccgctgagtt tcttgatgcg ggcagttata
     3001 agcatgtaca acgctgggcg aaagaagtag gcgaacgtcc ggcggtgaaa cgtgggcgta
     3061 ttgttaaccg caccaacgga ccgctgaatg agcagttgca tgagcgccat gacgccagtg
     3121 atttcgagac gaatacggaa gataagcgtc aggggtaagg gttggtgttc gtcgcagcaa
     3181 gccatccagg ccggataagg cgttcacgcc gcatccggca atcgtgcaca atgcctgatg
     3241 cgacgctgcc gcgtcttatc aggcctacga aagctaatca tcacgcgctg gtaatgtcgt
     3301 aatccatttg gcgcagtgcg tctaatgtgg ctttggcttc atcttcgtcg atgatgctca
     3361 tggcaaatcc gacgtgtacc agcacccact ggcccagtag atcggcaggg ttaccttcac
     3421 aaatcagggc gatattcacg tcgcgcttga taccacatac ttcaacctgc gcaagcaggt
     3481 gaatatcttc accgacagcc agcacctggc ctggaacgcc aatacacatt attaactccg
     3541 gttattcaac ttcaatactt ttgacgatca gcgaatcgcc ggtatcgaca cgcaaccgct
     3601 cgccgtgaca gagcggacac tgcgcatcgt gctgatgaat ctccaccacc tggctgcaat
     3661 cccagcacca ggcctgggcg ggtttataga cgatatgtaa atcgcaccct tgcgccaccg
     3721 ttccgtggca gacaatttca aaactaaaac ggacggcgct ctcctcaacg caggagagcg
     3781 cgccaatttc cagccacacg gcggtgacgc gcttaacatc gtgctgctcc gcctgccgtt
     3841 ggataatttc aacggcgctc tggcaaagcg acaactcatg cattttcgcc actcctgcga
     3901 ccaaacagca gggcgcgacg ccctgcgtgt ggtacatccg gattcgtgac tggcagcgaa
     3961 aggatcattc gtgcgcagtc atccgtcagg cgttggccct cttcaatcga catactgtgc
     4021 gaaagcggtg acatcagcga acaggagagg tattgcgaaa caccgtccag ttcgccaacg
     4081 gtaaaggtca tggtgccata cggcagttgc agaccaattt tttcactcac tttgcgcagc
     4141 ggccagagtt gatccgggcc ggggaagata actgcactca gcatccacgg ggtgatcaca
     4201 caccccgtcc actgaccttc gaacagcgta aaatcagaaa catacaccgg cattgaagga
     4261 tgcagaaaag agagatcgtg catcgaacgc cgggcaattt cttcaaacgc tgcctgtact
     4321 tgcgccttcg gggaggtctg gaaacctgct atctcttcag tcatgaatcg cctcccgtgg
     4381 gatggcttcc acgcctgatt cgcgcagcgc agccagaacc tgctcaagcg caggttcaat
     4441 cattgcttca accgtcggcg ttaagccgat gtgtggctcc agcgattccg ggatcacgcc
     4501 gaccagggtc agctttttcg gaaactcacc ggtgaagcgc agggccgaca atacgtcggc
     4561 caggccaagc tgatgcggag agattttgtt ggtaaacaac gccggaactt cttcatcccg
     4621 caggatcatc atcgttcccg gcgtgttctt tttcgacaca atggcatccg caataatcag
     4681 atgatcgcga tttgccatgt cgccaagcag ctccattccc gccgtgccgc catcgaggat
     4741 ctcaacataa tccggcagaa tgtatcgttg ctctaacgct tcgacaatcc gcacaccgat
     4801 ggcttcatcg gtcagcaaaa tattgccgac ccctaagact aaaatacgca ttacagaacc
     4861 ttcactgaaa ccacttcgtt gccgtcggca tccactacgt gtaccgcaca ggccatgcac
     4921 gggtcgaagg agtgaatggt acgcaccact tccagcggtt tattcggatc agcaaccggt
     4981 gtacccacca gcgactgctc gtaaggaccg acgtcatcat tgaagttacg cggaccagag
     5041 ttccaggttg atggaacaac tgcctggtag ttactgataa taccgtcttt aataaccatc
     5101 cagtgagaga gcataccgcg cggcgcttcg aggaagccaa cacctttgaa ctcacccgtt
     5161 gccggaatgt tcggtttcac aaaggtggtg tgatcgcctt tgccgatatt ggtgatcagt
     5221 gcactgtatt gattttgcag gatatcctgc aattcgcagc agtgaacggt acgaccaata
     5281 atacggccca gcgtggagtg cagctgcgcc acttccagcg tgttgccagt cagtttctga
     5341 taaatcgcaa cgatttcatt cagtttgttc tgggtagatt cgcgacctgc cgccagtttc
     5401 accagcatat tagccagcgg acccacttct accgttttgc cgtagaaagt cggtgatttc
     5461 acccaggaat atttcccgtc gtcagaccaa ccatcataag ccggaatggt ggtaccttcc
     5521 cacggtgcct gcggcgcttc gtctttatac caggagtgct tcgcgctttc ctgaatgcct
     5581 ttgatcaggt attcatcgga atgagaagtg atcggacgat acgaggacag atccgcattc
     5641 tcaatgtagc cgcccgggaa caggaagctg ccgtttttac tgtcggtcgg gaattccggc
     5701 acgctcaggt agttcaccgc acctttaccg cgcgtcagcc attccgggta gaacgcggca
     5761 ataactgcgg tatcaacctt ataaacctgt tcaacaaagt cgctcagctt gtcgatgaaa
     5821 gacttgatgt acatcaggcg ctcaaggttc agcacgccca aaccgtcgag gttgatcggg
     5881 ttcgcgacac cacctaccgc caggttctga atgtgcggcg ttttaccgcc cagcagcgcc
     5941 acgacgcggt tagcgtcacg ctggcactcc agcgcctgca ggtagtgcgc taccgcaatc
     6001 aggttcactt ccggcggcag tttcatcgcc gggtgacccc agtagccatt ggcgaaaata
     6061 cccaactgac cgctggcaac cagatctttg atcttgttct gaactttagt gaactcttcc
     6121 ggactgttca gatgccaggt cgaaacgcct ttcagcattt cggaggcttt ggttgggtca
     6181 gcttgcagtg cagaagtgat gtccacccag tccagcgccg aaagctgata gaaatgaacg
     6241 atatggtcat gcgtggtgtg cgcagccaga atgatgttac ggatgtattg cgcgttaacc
     6301 ggaacgtcga tattcagcgc actttctgcc gcacgaacgg aagacagcgc gtgagtggta
     6361 gtacatacgc cacagatacg ttgcacaatc atccatgcat cgcgcggatc gcggtttttc
     6421 acgatctctt ccatgccgcg ccacatggta ccggaagccc atgctttcga aacgacgcca
     6481 ttttcgattt cgcaatcgat gcgtaaatgc ccctcaatac gggttaccgg atcaatagta
     6541 attctctggc tcatgctttg ctcgcctcat gacgattatg atcgttttgt tttaaaggag
     6601 gaagtatcgg cagtagacga atgagtacga tgtaagcgca aatctcaata gccacaaaac
     6661 caatagaaat caacagttct tccctggtcg ggaagtaggc gtaaccgccg cccgggttga
     6721 atgccaccag cgaataggtc agacgccagg ttgcacaacc taacagtgcg ctcagtgctg
     6781 acaggaacag catgcgggaa tcattacgca gcttcgccac acgcagaacg accagcggga
     6841 agagcatcag caggacttca atccagaaca tcacggagta gaagtcaccg gcaaacgcta
     6901 acgacagctt gtcgcgatag ataagctcgc caaagcgcag cacgataaaa atcgccagca
     6961 acacactgat ggtgttggtc agcttgacga acagactctt ttcatccgga ccgttgccac
     7021 gcagacccgc ctgcaccagc gaaccttcaa agatgacaat cgagaagccc atgatgaacg
     7081 ccgtcagcag cgagaacagc ggcaacattt catagctctg ccacaacgga tgcaccttgt
     7141 agcccgccga gatcatcagt gaccccattg aagactggtg catggtcggc aacagcgcgc
     7201 caagtgcaat gatgaagaac atcaccttgt tcagacgctt gagagaaacc ttccagccca
     7261 gacgttcaaa cagtgccgga gcaaactcca gtgccatcac gccgatatag atggtcatac
     7321 agaccgccgt ctcgaacagt accgagttca cgttgaagtg acccggaatg tagaagtacg
     7381 gcaggttcca gtagcgaccc acgtcgatag tgatcgacaa gccacccagt gagtaaccaa
     7441 acagactcgc caacagcgcc ggacgcacca gcggatggta ttgcccacgg ttaaagacgt
     7501 ataccgccca cgccagcgcc cagccgccac aggcaaagcc ggtaccaatc agcaggtcaa
     7561 acgcgatcca cacgccccac gggaagccgc cgttcaggtc agagacagag cccagaccga
     7621 acaccagacg cttcacaatc aggagcatac agatgacgat taacggtcca aaaatcatga
     7681 ccggtttact gatgattttg ccgcccagcg gttgtggatc atgactcatg atcgtctcct
     7741 ccgtcgtgat ggtcgttttt ggtgttgcga cgaaccagca cggttaagcc cgccagcaca
     7801 gccagtggta gcatcatgcc tttatacagg gtgtgttgaa tattttcgga acgcgcaccg
     7861 gtagaaagat cgtccagttt cggcaggtcg agattttcat aaggcacacc cgtcagtacc
     7921 agaacctgag taccgccgcc ctctttctcg ccgtacagat gcggataata tttcggcacc
     7981 gtatgcaggt aagtgtcgcc agatttcagc gtctgacgcg gatagtggta ttcgctgcca
     8041 ggcttcagcg ccagacgttt tttcgcctcc gccatcagct cttcacgcgt accgaaaatc
     8101 accgcgcccg ccgggcacac ttctacgcag ccaggcagac cgcctttatc gagacgttcc
     8161 acacctttct ggttgcacag ctcgcactta tgcagcgcac caaacgggtt gttgtagtcg
     8221 tacttcggca cgttgtacgg acaggcgacc atgcagtaac ggcaaccggt gcacacgtct
     8281 ttgtcgtaat ggacaatgcc ggttttcgga tcttttttca gtgcagagac cgggcacaca
     8341 gagacacagt tcggatcgac gcagtgcata cactgtttct taatgtacgc gtagccgttc
     8401 tcctcctggt ctttgttgac ccctgtgccg ctggtccaca cctgaatgat gttattggta
     8461 tacggcgaca gtttgtcgtt gttcgaccag gtctgttccc cttgcgggtt acgttcaggg
     8521 aagttgatat cctgacactt ggtgacgcaa gcctggcagc ctacgcacaa ggtcgagtcg
     8581 tacaacatcc ccagcgatcc cggaattggc gggcggtttt cagcagccgc atgactgaca
     8641 gacggcagcg cgcccgtcag caatgccccg caggaggctg ctttaataaa attacgtctg
     8701 ttcacggtta ttctccccgt gagtcagcgt tatctttctt ttgctgacga cccagttcac
     8761 gcaccgccat cacgctgaca ccggcaacca gcccaaccac accgccgagc aaaccaatag
     8821 cgcctgcaga gacgttaccg ccctctttag cgttaacatc cggtttctgt gaacgcggag
     8881 tctgattttc gacgttggca agctgatgga tgcctttatg gaagccgata ccttcttcgt
     8941 tacagccata gcatgggtga ccaatcgcta ccggccacac gccgccaaca tcgcagaatt
     9001 gcagcgttga gcagttgccg taagtttctg gccctttaca gccgaggtgg tacaggcacc
     9061 agccttcacg gtggccttca tcaccgaact ctttggcaaa acgaccagca tcgaagtgcg
     9121 ggcgacgttc gcagtgttcg tgaatcagac ggccataggc gaaggtcgga cgatttttgt
     9181 catccagttt cggcggtttg ccgtaagtga tgatgtgcgc aacggtcgcg aggaagttgt
     9241 gcgggttcgg cgggcagccc ggaatattga taacggtttt gcctggcaga acttcttgca
     9301 ggctgactgc gccagttggg ttaactccag ctgcggcaac accgccccac gcagagcagg
     9361 aaccgatagc gataatcgct gctgcgcctt ctgccgcttt gcggatgtga tccacaatcg
     9421 gctcaccagc aaccatgcaa taaataccgt tatcttttaa tgggatggaa ccatccacaa
     9481 ccaacacata ctgccctttg tacttctcaa gtgcgttatg tttgttctct tcgacctgat
     9541 gaccgaaggc ggcggaaagc acttcgtgat actccagaga gatagtctcc agcacgaggt
     9601 tttctaccgt tggatgcgtt gcacgaagca gagattccgt acaaccggtg cactcctgcg
     9661 cgccaatcca gataactggc ggacgctgcg ggttagtaac cgattcggcc atctctgcag
     9721 cggctttgct acttaacccc atggtggcgg ctaatgctgc acaaagcttc atgaaatcac
     9781 gacggttaat gccgtgagaa tggatgaggg tgttatctcc agtcatttat agttattccg
     9841 ttgcgaagac ctggcatata ttttgcctca atcgcaaaat caataatgcg atcgatgcgc
     9901 catttaccac acatttatta tggttatcga tatcatgata ctgcggcggg gcgctaaaac
     9961 gaagggaaga agcattagtg taattaatta aagcaagata atacgtatgt ttgatcaatt
    10021 ttcgaggcga taaccggcca taaaaaagcg caacgtagaa caggaattat tctcacgaaa
    10081 gaaatccatt accatctctt tatccagtcc atagcggcgc gtcagaatac ccgcttccca
    10141 ggcgctaagc tggcgatctc cggtcttttc gtacatccgg tgtgagtagc ctaatacaaa
    10201 accgcgttta taatcggcac agaattttgt cacattaacc gcactgtcgg catgcgttgc
    10261 ttttaatccc gccattaagc ctttaccaaa atggttattc atcgttcacc tcattcgcta
    10321 tatattatca tatatagcaa tattttaggc aaagaacgac ttttgcgcac gctatttttt
    10381 catgcttgtt taagacgtaa tcccgtaaga gataaaccca tcaggagaga tatttatcga
    10441 accatttcag cgtcctttgc caggcaagat cggcggcaga tttgtcataa cgaggcgtgg
    10501 aatcattatg gaatccgtga ttaacccccg gatagatata cgcctcatag accttattat
    10561 tggctttcaa cgccgcctca taagcgggcc agccctcgtt gattcgggtg tccagttccg
    10621 cgaagtggag tagtaaaggc gcttcaatct tcgccacatc ggcagtgggt gcctgacgac
    10681 cataaaacgg caccgcgcag gccagttccg gatacgcgac agccgccgcg ttcgatacac
    10741 caccgccata gcaaaatccg gtaataccca cttatgcctg tcgcttgcgg atagcgttgc
    10801 ataaactcaa ttgcggcaaa gaaatcattc atcagtttgg ttggatcaac ctgctgttgc
    10861 agctcacgac ctttatcatc atttcccgga taacctc
//
LOCUS       DAJTMC010000142        10700 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.142, whole genome shotgun sequence.
ACCESSION   DAJTMC010000142 DAJTMC010000000
VERSION     DAJTMC010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10700
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.142"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(89..238)
                     /gene="hokB"
                     /locus_tag="OO130_003247"
     CDS             complement(89..238)
                     /gene="hokB"
                     /locus_tag="OO130_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HCR5366537.1"
                     /translation="MKHNPLVVCLLIICITILTFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            637..2250
                     /gene="trg"
                     /locus_tag="OO130_003248"
                     /pseudo
     CDS             637..2250
                     /gene="trg"
                     /locus_tag="OO130_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
     gene            complement(2288..3210)
                     /locus_tag="OO130_003249"
                     /pseudo
     CDS             complement(2288..3210)
                     /locus_tag="OO130_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            3427..4770
                     /locus_tag="OO130_003250"
     CDS             3427..4770
                     /locus_tag="OO130_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR5366538.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGPLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GGPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEYRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHLGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            4995..6650
                     /gene="opgD"
                     /locus_tag="OO130_003251"
                     /gene_synonym="mdoD"
     CDS             4995..6650
                     /gene="opgD"
                     /locus_tag="OO130_003251"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HCR5366539.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            6790..7014
                     /locus_tag="OO130_003252"
     CDS             6790..7014
                     /locus_tag="OO130_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HCR5366540.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGFDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKGV"
     gene            7077..7613
                     /gene="rimL"
                     /locus_tag="OO130_003253"
     CDS             7077..7613
                     /gene="rimL"
                     /locus_tag="OO130_003253"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HCR5366541.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(7608..8588)
                     /locus_tag="OO130_003254"
                     /pseudo
     CDS             complement(7608..8588)
                     /locus_tag="OO130_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8712..9704
                     /gene="tehA"
                     /locus_tag="OO130_003255"
     CDS             8712..9704
                     /gene="tehA"
                     /locus_tag="OO130_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HCR5366542.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATMPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSENGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            9701..10294
                     /gene="tehB"
                     /locus_tag="OO130_003256"
     CDS             9701..10294
                     /gene="tehB"
                     /locus_tag="OO130_003256"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HCR5366543.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDRQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKLGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            10597..>10700
                     /locus_tag="OO130_003257"
     CDS             10597..>10700
                     /locus_tag="OO130_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HCR5366544.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYSGF"
BASE COUNT         2737 a         2497 c         2711 g         2755 t
ORIGIN      
        1 aaacatgcac taactgaagt gtaaaagggg tgccatgaga aaatgaatct gctagtcaga
       61 tgcgcgggga aaatccccgc gcttgccctt acctggacgt gcaggccatg agcgcagcaa
      121 cctccttatc accgtcccgg aaccgcagtt cgtagagcgt ttgtcgggtc aggagtgtga
      181 atgtcagaat cgtaatgcag ataatgagca gacacaccac cagagggttg tgcttcatag
      241 cctttctcct tgccggatgg cgggtaagag gctaagatct gaattgctag gttcattcgt
      301 tggcctcggt tgatagaaat atcggtcggg gccttcgtct ttctgattcc cggttagcct
      361 gaaaacagaa agtctcaggc acccgcaggc atcctatgag gtttccttag ggacgaaaat
      421 aatcacttca cgaaattgcg tgctgttttc cagaattttt cgtcattcgg attagccagt
      481 ttagccattc gttactctct tcattccaat agcattaatt ttctctgcaa taattgttgt
      541 aaaaatgtga cgcaaagagg tttttggtca taagtaatta ccgtcaagtg ccgatgactt
      601 tctatcagga gtaaacctgg acgagagaca acggtaatga atacaactcc ctcacagcga
      661 ttaggttttt tgcatcacat caggctggtt ccgttatttg cctgcattct tggcggtatc
      721 ttagttctat tcgcattaag ttctgctctg gcaggccgat cgcgatcagc gtgatgttac
      781 tgcggagatt gagattcgga ccgggttagc gaacagttca gattttttgc gttcagcccg
      841 gatcaatatg attcaggccg gggctgcgag tcgtattgcg gaaatggaag caatgaagcg
      901 aaatattgcg caagccgaat cggagattaa acagtcgcag caaggttatc gtgcttatca
      961 gaatcgaccg gtgaaaacac ctgctgatga agccctcgac actgaattaa atcaacgctt
     1021 tcaggcttat atcacgggta tgcaacctat gttgaaatat gccaaaaatg gcatgtttga
     1081 agcgattatc aatcatgaaa gtgagcagat ccgaccgctg gataatgctt ataccgatat
     1141 tttgaacaaa gccgttaaga tacgtagcac cagagccaac caactggcgg aactggccca
     1201 tcagcgcacc cgcctgggtg ggatgttcat gattggcgcg tttgtgcgtg ccctggtcat
     1261 gacgctgata acatttatgg tgctacgtcg gatcgtcatt cgtccactgc aacatgccgc
     1321 acaacggatt gaaaaaatcg ccagtggcga tctgacgatg aatgatgaat cggcgggtcg
     1381 taatgaaatc ggtcgcttaa gtcgtcattt acagcaaatg cagcattcac tggggatgac
     1441 agtagggact gttcgacagg gcgcggaaga gatttatcgt ggcaccagcg aaatttcagc
     1501 tggcaatgcg gacctgtcat ctcgcaccga agaacaagcg gcggctatcg aacaaactgc
     1561 cgccagcatg gagcaactca ctgcgacggt gaaacagaat gcggataacg cgcatcatgc
     1621 cagcaaactg gcgcaagagg cttctattaa agccagcgat ggcgggcaga cggagtaaaa
     1681 acgatgggcg ctatctccac gagttcgaag aaaatttctg agatcaccgc cgtcatcaac
     1741 agtattgctt tccagacgaa tattctggca ctgaatgctg ccgttgaagc cgcgcgagcg
     1801 ggtgagcaag ggcgtggatt tgccgttgtc gccagcgaag tacggacact cgcaagtcgc
     1861 agcgctcagg cggcgaaaga gattgaaggc ttgatcagtg aatcagtcag gttaattgac
     1921 ctggggtcgg atgaggtggc aacggccggg aaaaccatga gcactattgt tgatgccgtc
     1981 gcgagtgtca cacatatcat gcaggaaatc gccgccgcct cggatgaaca aagtagaggc
     2041 ataacgcagg ttagccaggc gatttctgaa atggataagg tgacgcaaca gaatgcttct
     2101 ctggtagaag aggcctcagc ggcggcggtg tcccttgaag aacaggcggc acgattaact
     2161 gaggcggtgg atgtattccg tctgaacaaa cattctgtgt cggcagaacc tcgcggagcg
     2221 ggtgaaccag ttagtttcgc tacggtgtga aaatgttcaa ggagggatcg acagatccct
     2281 tcacctttca gaacggcatt gattttcgaa tagcgttaat catcaactgg caaccagaag
     2341 agaacatcgc atctacgcgg gtcagtattc caatcggttc gcctgcacca tgtcccggaa
     2401 caggctgggc caccagcgtg gcatgacgca ggtcgtcttt taccgcgcca gaagggacaa
     2461 accacacgta gtcgtattca accgtaagtt gacgagatag cgaagcagac agcgtttcga
     2521 tacaacccga aggaatttta catccctggc tctgtactaa tgcatctgaa tgctggcgtg
     2581 gcgcagtgcc ttctggtgat acaacgaccg gccatttcag cactcggctt agcgttacgt
     2641 tctcctgaag tagcgggtga ttagggcgga caaccagctt cagcgattca agaaacagca
     2701 gttcgtaatt aagcccggtc atcagttcag gatctgacat ccgaccaatg ccgatatcga
     2761 tttccccggt tttcaaaccc gccagaatca tagggttact cattgtcgca acttgcaagg
     2821 tcgtctcttt ttgttgctga tgaaactgac ctataaccga aggtaatatc cccagtgccg
     2881 cagtaggtag tgcaccaatc ctgacgacat cattattaag accttcttta cgatgaagcg
     2941 actgtccggc agtgttgatg gcgtcaagaa ctctgactgc atgcgttaaa aattgttcgc
     3001 cgggtaaggt aagttgcgcc ccctgacgac cacgctcaaa caagcgagcg cccgtcagct
     3061 gctacagttc attcaatgtc ttagagagcg caggttgact caaattaagg gtttcagccg
     3121 cgcccccaaa gttccttgtt gtgcgacagc tacgaatgta tgaaggtggc gcaaacggat
     3181 gcgctgacta aacagactat ttttttccat aagcgatgtt aaaaacgaag cggtgtcgct
     3241 gacaagtgaa gttgtttgat tatgataact tgattgcaaa atataattaa caattaaagc
     3301 aattgtgtta cagcaaaatg gataatattg atgttttcgc ggcgagatca cagtttgtaa
     3361 attcttcccg ccagagtgaa tgcggttacc tacactccag attactgacc actggaggca
     3421 gacactatgg cgaacagcat cacggcggat gagattcggg aacagttttc gcaggcaatg
     3481 tcagccatgt accagcaaga agttccgcag tacggcccgc tgctggaact ggtagctgat
     3541 gtgaatctgg ctgtgctgga aaacaatcct caactgcacg aaaaaatggt aaatgcagat
     3601 gagctggcgc gactgaatgt tgaacgtcat ggggcgattc gcgttgggac tgcacaagag
     3661 cttgctactc ttcggcggat gtttgccatt atggggatgt acccggtgag ctattacgat
     3721 ctctcgcagg caggggggcc ggtacattcg acagcatttc ggcccattga tgatgcttct
     3781 ctggcgcgta atcccttccg cgtttttacc tccttactcc gccttgagct tatcgagaac
     3841 gaaattttgc gccagaaagc ggcggagatt ctgcgtcagc gcgatatctt caccccacgt
     3901 tgtcgacaac tgttagagga atatgagcag cagggcggtt ttaacgaaac acaggcacag
     3961 gaatttgtgc aagaggccct ggaaacgttt cgctggcacc agtcagcaac ggtagatgaa
     4021 gaaacctatc gcgccttgca caacgaatat cggttgattg ctgatgtggt ctgttttcct
     4081 ggatgccata tcaaccacct gacgccacgt acgctggata ttgaccgggt gcagtcgatg
     4141 atgcctgaat gcggaattga acccaaaatt ctcatcgagg ggccgccgcg ccgcgaggta
     4201 ccgattttac tacgccagac cagctttaaa gcactggaag agacggtgtt gtttgcgggg
     4261 cagaaacagg gcacgcatac cgcgcgcttt ggtgaaattg agcagcgtgg cgtggcatta
     4321 acgccgaaag ggcgacaact gtatgatgat cttctgcgta acgctggaac cgggcaggat
     4381 aatctcactc accaaatgca tttacaggaa accttccgca cttttcctga cagtgagttt
     4441 ttaatgcgtc agcaagggtt ggcatggttc cggtaccgtc tgacgccttc gggtgaggcg
     4501 catcgtcagg cgattcatct tggagacgat ccacagcctt taattgaacg tggttgggta
     4561 gtggcgcaac ccatcaccta tgaagatttc ttgcccgtta gcgcggcggg gatcttccag
     4621 tcaaatctgg gtaatgaaac gcagacacgc agtcacggta atgccagtcg cgaagcattt
     4681 gagcaggcgt tgggttgtcc ggttttggat gagttccagc tttaccagga agcggaagaa
     4741 cgcagtaaac gtcgctgtgg tttgctttaa attctgacca tccgcctttg caaaaatttg
     4801 cctgatttta caaacgaatc aggctcatcc catcgacata aaaaaaatgc cgatttatgc
     4861 atattctctc agttcaacaa ttggattatt aataaatatt gtctagagtg agcggtcata
     4921 aataagcact ttcttgccgc tgaaaacgac cagcgcggga ccattcacaa caccagaagg
     4981 actcactttc aggtatggat cgtagacgat ttattaaagg ttcaatggct atggccgccg
     5041 tgtgcggtac cagcggcatt gcttctcttt tttctcaggc ggcattcgcg gcagattctg
     5101 atattgccga cgggcaaacc cagcgttttg acttctccat tctacagtca atggcgcacg
     5161 acttagcgca aacagcgtgg cgtggtgcgc ctcgtccgtt acctgacacg ctggcgacaa
     5221 tgacgccgca ggcttataac agtattcaat acgacgccga aaaatcgctc tggcataacg
     5281 ttgagaaccg tcaactggac gctcagttct tccatatggg aatgggattc cgtcgccgcg
     5341 ttcgtatgtt ttctgtagat ccagcaacac atctggcgcg tgaaattcac tttcgcccgg
     5401 agttgttcaa atacaacgat gcgggtgttg atacaaaaca attagaaggg caaagcgatc
     5461 tcggctttgc cggttttcgc gtgtttaaag cccccgaact ggcgcgccgt gatgtagtat
     5521 catttctcgg cgcgagttat ttccgcgccg ttgatgatac atatcaatac ggtttgtcgg
     5581 cccgcggcct ggcgatcgac acttacaccg acagtaaaga agagttcccc gactttaccg
     5641 ccttctggtt tgatacggta aaaccggggg caactacctt taccgtttat gcgttgctcg
     5701 atagcgccag cattactggt gcctataagt tcactatcca ttgtgagaaa agtcaggtga
     5761 ttatggatgt ggaaaatcac ctgtatgcgc gcaaagacat taaacagctg ggcattgcgc
     5821 cgatgaccag tatgttcagc tgcggtacta atgaacgtcg gatgtgcgat acaattcatc
     5881 cgcaaattca tgactctgat cgtctgtcca tgtggcgggg caacggcgag tggatttgcc
     5941 gtccgctgaa taatccgcaa aaattgcagt tcaatgctta caccgacaac aacccgaaag
     6001 ggtttggttt attgcaactg gatcgtgact tctcccatta tcaggacatt atgggctggt
     6061 ataacaaacg cccaagtctg tgggtggaac cgcgtaacaa gtggggtaag ggcaccatcg
     6121 gcctgatgga aatcccaaca acgggcgaaa cgctggataa cattgtctgt ttctggcagc
     6181 cagaaaaagc tgtaaaagca ggtgatgagt ttgcattcca gtatcgtctg tactggagtg
     6241 cgcaaccgcc tgttcattgc ccattagcgc gcgttatggc gacgcgtacc ggcatgggcg
     6301 gtttcccgga aggttgggcg ccaggtgaac actatcccga aaaatgggcg cgtcgttttg
     6361 ccgtcgattt cgttggtggt gatctgaaag ctgccgcgcc aaaaggcatt gagccggtga
     6421 ttacgctttc cagtggggaa gcgaagcaaa tcgaaattct ctatattgaa cccatcgatg
     6481 gttatcgtat tcagtttgac tggtatccga cttcggactc cactgatccg gtcgatatgc
     6541 ggatgtatct acgttgtcag ggggacgcta tcagtgaaac atggctgtat cagtatttcc
     6601 cgccagcgcc ggataaacgt cagtatgttg acgaccgcgt gatgagttaa ttgttttttc
     6661 ttcggcacct tcttcgggag gtgccgtctg gttaaacacg atcccgctcg catttttccc
     6721 taagttaaat gagtaatctg atggtgtgta tttcagatac accttgtcag ccactaacag
     6781 ggagtgcgta tgtttccaga ataccgagat ttaatatccc gtctgaaaaa cgaaaatcct
     6841 cgctttatgt ccttgttcga taaacacaat aaacttgatc atgaaattgc cagaaaggaa
     6901 ggtttcgacg gtcgagggta caatgcggaa gtggtccgca tgaaaaaaca aaagctacag
     6961 ttaaaagatg agatgctcaa aatcctgcag caggagagcg tcaaaggggt gtaaactttc
     7021 ctaagccgcc agccaggcgg cttttttaac aacagcatgg attgactgga gataagatga
     7081 ctgaaacgat aaaagtaagc gaatcacttg aattacatgc tgttgcggaa agtcacgtca
     7141 cacctcttta tcagttaatc tgtaaaaata aaacctggtt acagcagtcg ctaaactggc
     7201 cgcagttcgt tcagagtgaa gaggacacgc gaaaaacggt gcagggtaat gtgatgttgc
     7261 atcaacgcgg ctatgccaaa atgttcatga ttttcaaaga agatgaactt atcggcgtta
     7321 tctcgtttaa tcgtattgaa ccactgaata aaactgctga aatcggctac tggctggacg
     7381 aatctcatca ggggcagggg atcatttctc aggcgctgca ggcattgatt catcattacg
     7441 ctcagtctgg tgaacttaga cgcttcgtga tcaaatgtcg ggtggacaat ccgcaaagca
     7501 accaggtcgc tttgcgcaat ggttttatcc ttgaaggttg cctgaaacag gctgagttcc
     7561 tgaatgatgc ctatgatgat gtgaacttat acgcgcgtat tatcgattca taataaccct
     7621 gccagcggcg ttcgcgtaat gcgctcctcg ccgttaatga cttttggacc acggacatga
     7681 accgtgtcgc catcaaaggc ttcaacgacg gcgtggtcgg ttaactccac atggttttca
     7741 attatcaccg cacctgaaat acggctctca ccttgtatta caacgtgctc gtcgagcaga
     7801 atcggttcac cacgtaccac ggcatttccg ccaatcaaga catgatgttt taacacacaa
     7861 ttaccttcca caatggcata ttccgccacc tgcgaactgt aatgaatcgt cggaatggca
     7921 tcttcttcta tgccagcttt cacctgcgcg tgaccgtaga ctttagcgca atcgcatagc
     7981 cagacgttgt tctcttcatt accttcaaca ctggcaaaat caaaaacttc ggcgcgatgt
     8041 tcaataaaag cataccggac gacggcatcg ccataaattt gtgcctggtg gacaatacgc
     8101 gaggcgctaa cccttgcgcg atcataaatt tggagcagga gttgatggtc gggcgttaag
     8161 ccttgtgcgg cgacgatcat agaatgttga tcaattaagg cgtgaccaaa tattctacat
     8221 tgcccgtaaa ctaatgagtc atgaatggtg acactgtcac ttatatatgc accctgactg
     8281 atttcactat tatcgatcca gacgttatcc gttgcgtaaa cctctcccca cagcacgctg
     8341 gtgccggtga tacgcgtatt accagaaatc accgttccgc cgaacgcaat agcattctgg
     8401 tcgtaaatcc agcaatttcc ttcctatgct aacaccgttt cgcgatcgat ccagccgcca
     8461 gcggttcctg ctatcacatc gttaaaatcg ctaatggcga taatctgccg taataacaca
     8521 ctttttttag tgccatcttc ctgataacta aaggctcgct gttcttcact tagacgatat
     8581 ttgcgcatag cgttttctca caggtgactt actataaccg tagcaaattc tgcggctctg
     8641 gctatgctcg agaaaattcc ataaaatgca tttcaaatat actttataaa ttaaacaaaa
     8701 tgagtaagaa gatgcagagc gataaagtgc tcaatttgcc ggcaggctac tttggtattg
     8761 tgttggggac gatagggatg ggatttgcct ggcgctatgc cagccaggtt tggcaggtca
     8821 gccactggtt aggggatggg ctggtgattc tggcgatgat catctgggga ttattaacta
     8881 gcgcatttat tacccgactc atacgctttc cgcatagcgt gctggcggaa gttcgccatc
     8941 cagtgctgag cagttttgtg agtttgtttc cggcaacgac gatgctggtg gcgattggtt
     9001 ttgttccgtg gtttcgccca ctggcggtgt gcctgttcag ttttggtgtc gtggttcagt
     9061 tggcttatgc cgcctggcaa actgcgggat tatggcgcgg atctcaccct gaagaagcta
     9121 ccatgcctgg actgtatctg ccgacagttg ccaacaactt tatcagcgca atggcctgtg
     9181 gtgcgttggg ctacaccgac gccggtctgg tgtttttagg cgcaggcgtt ttctcatggc
     9241 taagccttga accggtgatc ttgcagcgtc tgcgcagttc gggagaatta cccacggcac
     9301 tgcggacatc actcggcatt cagctcgctc ctgcgctggt ggcctgtagt gcctggttga
     9361 gcgtcaacgg cggcgagggt gatacgctgg cgaaaatgct ttttggttat ggactgctgc
     9421 aactgctgtt tatgctacgt ctgatgccat ggtatctctc ccagccattt aatgcttcat
     9481 tctggagttt ctcgttcggc gtatctgcac tggcaaccac cggtttgcat ctggggagtg
     9541 gcagcgaaaa tggatttttc catacgctgg cggtaccgct gtttatcttt accaatttta
     9601 ttattgcaat actgctcatc cgtacttttg cgcttctgat gcagggaaaa ttgttagtca
     9661 gaaccgagcg cgctgtttta atgaaagcag aggacaaaga atgatcattc gtgacgaaaa
     9721 ctattttact gataaatatg aattaacccg cacacactct gaagtactgg aagcggtgaa
     9781 agtggttaaa ccgggtaaaa cgctggatct gggctgtggc aatggtcgta acagtcttta
     9841 cctggcagcc aatggttatg atgttgacgc atgggataaa aatgccatga gtatcgccaa
     9901 tgtagagcgc attaaatcca ttgaaaatct ggataattta cacacccgag tcgttgatct
     9961 gaataacctc acatttgata gacagtacga ttttattctt tcgactgtgg tactgatgtt
    10021 ccttgaggct aaaaccatcc cagggctgat tgccaatatg caacgttgca ctaaacttgg
    10081 tggttacaac ctgattgtgg cggcgatgga taccgctgat tatccatgta ccgtcggctt
    10141 cccgtttgcc ttcaaagagg gagaattacg tcgatattac gaaggctggg agagggtgaa
    10201 atacaatgaa gacgtcggcg agctgcaccg caccgacgcc aacggtaatc gtattaaact
    10261 gcgtttcgcc acgatgctgg cacgtaaaaa atgacccgct aagaataagg cgcgtgaaaa
    10321 ttccccacgc tgagatgatt tactgttctt cttttcggta agcatatttt ttatcgaagg
    10381 gatgtgaaat taatcacagt agtcgaagtt ttttagcagc ttaacttact gaaatttaac
    10441 tactgatgat tgacttagcc cctttttcgg cattgactat gtcgtctgaa aaggggctga
    10501 aaaatttatt ttcaccaaca ctttttttgc cacaacacga agcggcgctt tttgctataa
    10561 cttaaaaagt aatataatca tctcaggaaa ctattcatgc gtaccacatc atttgcgaaa
    10621 gttgcagctt tatgcggctt gttggctctg tctggttgtg cctctaaaat cacccagcca
    10681 gataaatatt ctggtttttt
//
LOCUS       DAJTMC010000143        10661 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.143, whole genome shotgun sequence.
ACCESSION   DAJTMC010000143 DAJTMC010000000
VERSION     DAJTMC010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10661
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.143"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            311..1609
                     /gene="kgtP"
                     /locus_tag="OO130_003258"
     CDS             311..1609
                     /gene="kgtP"
                     /locus_tag="OO130_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HCR5366545.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1606..1929)
                     /locus_tag="OO130_003259"
     CDS             complement(1606..1929)
                     /locus_tag="OO130_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HCR5366546.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(1975..3330)
                     /gene="pssA"
                     /locus_tag="OO130_003260"
     CDS             complement(1975..3330)
                     /gene="pssA"
                     /locus_tag="OO130_003260"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HCR5366547.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYKAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENSGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRLFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3444..6104)
                     /gene="pat"
                     /locus_tag="OO130_003261"
     CDS             complement(3444..6104)
                     /gene="pat"
                     /locus_tag="OO130_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HCR5366548.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQRKMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLHGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AISNPLDLRDDASSEHYIKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RIHGLLVQSMANRAGAQELRVVVGHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            complement(6136..6834)
                     /gene="tapT"
                     /locus_tag="OO130_003262"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6136..6834)
                     /gene="tapT"
                     /locus_tag="OO130_003262"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HCR5366549.1"
                     /translation="MTENTVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREALAEGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6903..7322)
                     /gene="trxC"
                     /locus_tag="OO130_003263"
     CDS             complement(6903..7322)
                     /gene="trxC"
                     /locus_tag="OO130_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HCR5366550.1"
                     /translation="MNTVCTHCQSINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7529..8566
                     /locus_tag="OO130_003264"
     CDS             7529..8566
                     /locus_tag="OO130_003264"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCR5366551.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAELVQSITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8614..9303)
                     /gene="ung"
                     /locus_tag="OO130_003265"
     CDS             complement(8614..9303)
                     /gene="ung"
                     /locus_tag="OO130_003265"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HCR5366552.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
     gene            9608..9991
                     /gene="grcA"
                     /locus_tag="OO130_003266"
     CDS             9608..9991
                     /gene="grcA"
                     /locus_tag="OO130_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HCR5366553.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10047..10634)
                     /gene="eamB"
                     /locus_tag="OO130_003267"
     CDS             complement(10047..10634)
                     /gene="eamB"
                     /locus_tag="OO130_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HCR5366554.1"
                     /translation="MTPTLLSAFWTYTLITAMTPRPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
BASE COUNT         2653 a         2704 c         2739 g         2565 t
ORIGIN      
        1 aaaggccatc cgtcaggatg gcctttttgc attggcgcag aaaaaatgcc tgatgcgacg
       61 ctgcgcgtct tatactccca catatgccag attcagcaac ggatgcggtt tccccaactt
      121 gcctacttcc atacgtgtcc tccttaccag aatttatcct taagctcctc aataaccatt
      181 ttcctgctaa ctaaattcat ggttaatgtt gcataataat acgtaacaaa tgtataacat
      241 tttctttacc aaaagaaata aacaaaagtg accaacaaaa gcatcggatt acggcaggag
      301 acataatggc atggctgaaa gtactgtaac ggcagacagc aaactgacaa gtagtgatac
      361 tcgtcgccgc atttgggcga ttgtgggggc ctcttcaggt aatctggtcg agtggttcga
      421 tttctatgtc tactcgttct gttcactcta cttcgcccac attttcttcc cttccgggaa
      481 cacgacgact caactactac aaacagcagg tgtttttgct gcgggattcc tgatgcgccc
      541 aataggcggt tggctatttg gccgcatagc cgataaacat ggtcgcaaaa aatcgatgct
      601 gttatcggtg tgtatgatgt gtttcggatc gctggttatc gcctgcctcc caggttatga
      661 aactataggt acgtgggctc cggcattatt gcttctcgcg cgtttatttc agggattatc
      721 cgttggcgga gaatatggca ccagcgccac ctatatgagt gaagttgccg ttgaagggcg
      781 caaaggtttt tacgcatcat ttcagtatgt gacgttgatc ggcggacaac tgctagccct
      841 actggttgtc gtggttttac aacacaccat ggaagacgct gcactcagag agtggggatg
      901 gcgtattcct ttcgcgttag gagctgtgtt agctgttgtg gcgttgtggt tacgtcgtca
      961 attagatgaa acttcgcaac aagaaacgcg cgctttaaaa gaagctggat ctctgaaagg
     1021 attatggcgc aatcgccgtg cattcatcat ggttctcggt tttaccgctg cgggctccct
     1081 ttgtttctat accttcacca cttatatgca gaagtatctg gtaaatactg cgggaatgca
     1141 tgccaacgtg gcgagtggca ttatgactgc cgcattgttt gtattcatgc ttattcaacc
     1201 actcattggc gcgctgtcgg ataagattgg tcgccgtacc tcaatgttat gtttcggttc
     1261 gctggcagcc atttttaccg ttcctattct ctcagcattg caaaacgttt cctcgcctta
     1321 tgccgctttt ggtctggtga tgtgtgccct gctgatagtg agtttttata catcaatcag
     1381 tggaatactg aaggctgaga tgttcccggc acaggttcgc gcattaggcg ttggtctgtc
     1441 atatgcggtc gctaatgcta tatttggtgg ttcggcggag tacgtagcgt tgtcgctgaa
     1501 atcaatagga atggaaacag ccttcttctg gtatgtgacc ttgatggccg tggtggcgtt
     1561 tctggtttct ttgatgctac atcgcaaagg gaaggggatg cgtctttagt gacgggtcag
     1621 ttgccagacg gtatagccgg tgcttgcacc ggcgacatcc caggccaaat ccttccagct
     1681 ccagccgctc ccttcggggc ggctatccca aagctctttt gacgccccca aactgacaga
     1741 gaacatcaat ccaaacatgg cactgcgatc ccggctcatc ccctgatgct gtgaatattc
     1801 atttccggcg gcggaaagca tcgccgaggc gataaagtgt tgagctttat cctgcccgct
     1861 ccagctatcg ttagccatat ggctgcatcc agaaagtaac aatagcgaac agacaaaaag
     1921 aatacgcaaa acgtggcctc caaaaaacaa accccgtaca ggacggggtt gtgattacag
     1981 gatgcggcta attaatcggt cgatgcggat acggcgcaaa cggcggatga gtttacgaac
     2041 cttcaccgga taatcggcaa tactttgcag atcgcgatag tgcttaacga tggtggtatg
     2101 ctcgcggatc agctccagtt ctttctctcg ctgcggcgca agctccagtt gtggatcgtg
     2161 gatcaaaatg gcgttttcca gatccagacg ccaggcgcgc gggttcaggt tattaccggt
     2221 gatcaacatc cacttatcat caacccacat ccctttcagg tgataggtgt tgtcgtcatc
     2281 tttccataac cgaaccacta gctggtcagt attgacgtaa tactgcaaac ggctcaggaa
     2341 aagacgcaga ttgatctcat agagataagg caatgcgcca attatcttga aaggttcatc
     2401 ttccggaatg tagaagtcgt tcgcggtttt atcaccaaca ataatttcga cctttttccc
     2461 ttcgcgcagc aactggataa tattgcgcac aaggattgct ggcaggttga agtatggcgt
     2521 acagatggtt agtttctgct cggcacaagg cataagatgg aaaatggtct tgttcaacag
     2581 actcgatttc cccagcccca ctagcggcgt tacggaaagc tgatcgttgt cggcatcgcc
     2641 ctggaaatga taagcggcat cacgcagctc ctggcggaac agacgaatat cgttcttgat
     2701 ttccgggctt tttggccgat taacatcatc cagacgatta acgccgcggc cattcataat
     2761 attctgtgta acccattcaa acataatgtc tgacatctta cggttacgga taagatgata
     2821 acggtcgtag cgatatttat cgtgctgatg caggtaaaca tcgttcaggc tggcaccgct
     2881 ataaagtacg ctatcgtcga tgataaagcc tttaaagtgc agaacaccaa gggcttcacg
     2941 ggtattgatt ggaacgccat aaaccggaac atctacgccc gaattttcct gcgccatgcg
     3001 gcagtaccag tcagcgttag tgttagatgc cgcagcgcca atgcgtccac gttgtgcacg
     3061 atgccagtcg accagcaccc gcacatccag ttccggacgc tgccttttag ctttatacaa
     3121 cgcgttcaga atgcctttgc caccgtcatc ctgttcgaga tacagggcga caatgcaaat
     3181 gcgctgcttc gcgctggcta ttttttccag cagcgtctcc cggaagtcgg cgggagcgta
     3241 aaagaaatcg acatcatcaa ctgattgaga aatcttgggt agttgggcaa ggtgttgttg
     3301 atgtttatta cgcttaaatt ttgacaacat cacagtgcat ttcttctctg ttcattgaag
     3361 gatcctctgt gcaatgcaga cgacataagc gggcaataat aacaccagtc ccgattaagt
     3421 agtcaacatt tccagtacct tactcatgat tcctcgcgtt gggcaagatt tagcgtaagc
     3481 ccaacgatcc cctcttcgag ctggatatca acgttaaacc cgagcttgcg ggctagcgcc
     3541 accatgccac gattgtttgg catcgtaata ccattcagac gttgtagtcc gtgatctcgc
     3601 gtataggtaa tcaacttttc cattaagcgt cgacctaagc ctaacccttt gagatccgag
     3661 cgaaccagca cagcaaattc ggcatcgatg ttatcaggat cggaaatcgc acgcgtgacg
     3721 ccgaggatct cttccgtttg atcaatacgt cgtaccgcta caaacgccat ttcccgatcg
     3781 tagtcgatct gtgtcatgtt ggctaaatct tcatgggtaa attcgttgat ctcgctaaag
     3841 tagcggtaat aaagatcttc tttggtgact cgcgaaatga attgctgaag ttgtggctca
     3901 tcttctggca aaatcgggcg gaacaagcag cgttcaccgt ttttcaattc tacccattct
     3961 tccagctgat gcggataagg gcgcactgcc agccgactct cgttatcgcc ttcaaacggc
     4021 gagatatcca gcgtgacatc cagcgcggta aattcactgc cagaagccag caaaggatga
     4081 atatccagac gctgaatttc cgggcaatcg acaatcaagt tggaaacctg caccagaagc
     4141 tggctcaagc ctgcaacatc caatgggcgt agcgcactgc gcgcacgaat ctttttactt
     4201 ttgatcccct gaataaccag atagcgggcc aggttcatgt tcagcggcgg cagtgcgacg
     4261 acggcttgat cttcaggacg ccactccaca ccgccttcac ccagcatgat caacggcccg
     4321 aaaaccggat cgtgcccaac cacaacccgc aactcctgag cgccagcacg gttagccata
     4381 ctttgcacca acaggccgtg gatccgcgcc tgcggccagg ccatttttac gcgatcgaaa
     4441 atggcgtttg ccgcttgctg gacttcattg gctgtacgca agtacagcat gacgccctga
     4501 acttccgatt tatgtggaat atccggcgaa cgcaatttca gcgccaccgg ataaccaatc
     4561 tgttcggcaa tatgcaccgc ttcggtgcta tcgctggcaa tccaggtagg gagcgtgttc
     4621 atgccatacg cttgcaggat gggctgaact tcatgggtat cgagcgacgt agccccttcg
     4681 gcaatcgcct gttgcaacag aagatgcgct tctgcggtat tggaagtcag attgctgggc
     4741 aacgccggcg tttcgcgtag ttgcttctga ttacgccggt actccaccat atgcataaaa
     4801 gcagtgatgg ttccttccgg ggtacggtag gtcggcagcc cggcttcgct gaataaacgt
     4861 cgtgcctctt gcgaggagtg ctcgccgcac cagttcgtca gcaaagagac atatttgctg
     4921 cggggatgat gctttaccgc ttcaattaat acttgcgcgc tttctgttgc gggagcagcg
     4981 gcgctgggcg aatgaataac catcagcgcg tcaaaatcct ggctgtggag cagaatatcc
     5041 agcgttttaa tatagtgctc actgctggcg tcatcgcgta gatcgagcgg gttagatatt
     5101 gccacatgtt ctggcagtgc atcgcgcagt ttctggcagg tttcttcgct tagcgttgcc
     5161 agcttgccat tgcgtgacca taaggcatcc agcgccagcg cggcaggcgc agcaccgttg
     5221 ctgataatca tcagccggtc gccatgtagc gggcgcatat ggctaagggt ttccaccgcc
     5281 gaaaacagct cgtgggtgtc ctgtacccgc aacaaaccgg cacgctgaat agccgcatcc
     5341 catgccgggt ccattcctgc cgtggtgttg agcagtcgct gtgccgccgg gctacgtccg
     5401 cttttaatca ccagaatcgg tttattacgc gaggcactac gggccgccga aacaaagcgt
     5461 cgcgcgtcgc ttaactgttc gagatagagc aggatggcgc tggttttact gtcgcgtgcc
     5521 agatagtcaa gcaattcatc aacgtcgata tccaggctgt cgccgagcgc aataaagtag
     5581 gaaaagccca tcttacgctg ttgcgcccag tcgaggatgg tgttggagac ggcagccgat
     5641 tgcgaaataa acgccagctt gccgcgttta atcggcacag gcgaaaagct ggcattcaga
     5701 ccttgccagg gagccagtaa acccagactg tttggtccaa gcaggcgcat gttatggcgc
     5761 agggcgcagg cgcggagatc ttcgtgttgc gatgccgggg cggaaagaat aatgcaggtt
     5821 ttacagcctt tctcgccgag ctcttccaga agagcaagat tacggctggc attggtacat
     5881 aaaaccgcaa ggtcgggtgt aaagggcaag ctggcaatat ccggccaggc caacacaccc
     5941 aacaccgctt tccaggctgg cgtcaccggg agtaccggtc cgttaaagcc tcccgccagc
     6001 aggttacgca tcatcaggta acctgcgcga ttgggtttca tcgacgcgcc aattaccgct
     6061 atcgattttg gtcgcagtag tgcttccagt cctcgctgac tcataccggt ctcccgcaga
     6121 agtgaccgaa tgattttaaa cgctttctaa ctgttctgct gtgatgctac ccagatgttg
     6181 cgttttccct gccagatagc gtgttttaaa gcgggtaaaa tgctcgccta accctgctgc
     6241 cgccccggta tcgccggcca tatctaacag tgcgatggct acctcggcag tacaatattg
     6301 gccttcagcc agggcttcac gcaggcgata ggcagaaagc cgggaaagat cgacggaaat
     6361 gacgggaaga ttatccagat acggactttt acgaaacatc ttgcgagctt ccggccaggt
     6421 accatcgagc atgataaaca gcggtggctt accggcaggt ggtgtgaaga tcacttcccg
     6481 ttgctcatca gcatacgagg cgggaaagac caccattggc tgataatccg ggttttgtac
     6541 cagatccagc aaatcctgcg agggttcggt acgcgaccat tgaaacgcaa cggtatcagg
     6601 caaaatatca gcaatcagac gcccggtatt actgggcttc attggctcgg tgtcgaacat
     6661 cagcaaacag aagcgacttt ttgcttgtgc tggggtaatt gtcgaacaga gacataattt
     6721 ctctggcaaa agacagcgtt ggcagcgacg aacgcgatta ccgcgggcaa gaaaaggacg
     6781 tgttgcgcgc gcaatacgct cggcgcgtaa ctggagaaca gtgttttcgg tcataagaga
     6841 gcgtcgaaaa aacgccattg tcgcagagga gaaaacgggg cacaagatgc gccccggtaa
     6901 gattaaagag attcgttcag ccagctatcg aacggagctt tcggtactgc gccattaagc
     6961 atgtcgacaa cctgaccgtt tttgaaaatc atgatcgtcg gtatactacg aattccaaag
     7021 cgactgctca attcacgttc agcttcggta ttcactttca caaagcgcac tttaccgcta
     7081 cgctcttgcg cgacatcttc aaaaattggt gcgaaattac ggcaggggcc gcaccacggt
     7141 gcccagaagt cgatcaccac aggtagatca tccttcagca atttgtcgag tgtttcaccg
     7201 gtcgcattaa tcacctctcc gtcaaacaag tcgtgaccgc agcgtccgca ttttgccgca
     7261 tcttcgatcc gatcgtcggg aatgcgattg atggactgac aatgggtaca aacggtattc
     7321 ataactaacc tcgggatgag taaggagact tatcggcaac gacgccggta tgtttctaat
     7381 atgttacata ttatcgggga cattgttcca gacaacaatc tgttttattc aataggtatg
     7441 atagtcgcag gctttggtac gaaatcatgg agaaccgcgc ggacttcggg taatctgcgc
     7501 gcttcgcgca gcgctggtgg agaaaagcat gaacgatgaa atgaaaggta aaagcggcaa
     7561 ggtcaaagtg atgtatgtcc gcagtgatga tgattctgat aaacgtaccc acaacccgcg
     7621 taccgggaaa gggggcgggc gtccaggaaa atctcgtgct gacggtggcc gtcgccccgc
     7681 ccgcgatgac aaacagagtc agccccgtga ccgcaagtgg gaagattcgc cgtggcgcac
     7741 ggtttcccgc gcgccgggtg atgagacgcc ggaaaaggcc gatcacggtg gcatcagtgg
     7801 taaaagtttt attgatccgg aagtgttgcg tcgtcagcgt gcggaagaaa cccgcgtcta
     7861 cggcgaaaac gcctgtcagg cactgttcca gagccgtccg gaagcaattg ttcgcgcctg
     7921 gtttatccag agcgtaacgc cacgttttaa agaagccttg cgctggatgg cggcaaaccg
     7981 caaagcgtac catgtggtgg atgaagctga attgacaaaa gcgtcaggca cggaacatca
     8041 cggcggcgtt tgcttcctga tcaaaaagcg taacggtaca accgtgcagc agtgggtaag
     8101 ccaggcaggc gcgcaggatt gcgttctggc actggaaaac gaatctaacc cgcataacct
     8161 gggtggcatg atgcgcagct gcgcgcactt cggcgtgaaa ggtgtcgtgg tgcaggatgc
     8221 ggcactgctg gagtcggggg cggctatccg taccgcagaa ggcggcgcag agctcgttca
     8281 gtcgattact ggcgacaaca ttgttaacgt gctggatgat ttccgtcagg caggttacac
     8341 cgtagtgacc acttccagcg agcagggtaa accgctgttc aaaactagtc tgccagcgaa
     8401 aatggtactg gtgctgggtc aggaatatga agggttaccg gatgccgcac gcgatccgaa
     8461 cgatctgcgc gtgaagattg acggtacggg caacgttgcc gggctgaata tctctgtcgc
     8521 aaccggcgtt ttgcttggtg aatggtggcg tcagaataaa gcctgataaa tcagccgggt
     8581 ggcaactctg ccatccggca tttccccgca aatttactca ctctctgccg gtaatactgg
     8641 catccagtca atcggcgtct cgccacgttg ttccagccac tgatttgcca gcacaaaatg
     8701 gttgcagcca aagaatccac gatgcgccga aagcggcgac ggatgcggtg ctttcagtac
     8761 atgatggcgt tgcttatcta taatcgcccc tttcttttgc gcatgtgatc cccacaacaa
     8821 aaacaccacg ccttcgcggt gctggttaat caggctgatg actttatcag taaacgtctc
     8881 ccaaccgagg ctggcgtggg aatgcgcctg acctgcgcga accgtcaaca cagtattgag
     8941 cagcagaacg ccctgacgcg cccagctttc gagatagcca tgattagggc gggtgaagcc
     9001 cggaatagta ttttccagct ctttatacat attcaataat gacggaggaa tggcaatgcc
     9061 gggacgaacg gaaaatgcca gaccatgcgc ctgtcccggt ccgtgataag gatcctggcc
     9121 gagaatcacc actttaacgt cacccaactc ggtaaagcgg aacgcgttaa agacatcttt
     9181 ttgtggtgga tagatagtga cgccggactg ccgctcgctg gcgacggtct gaagggtatt
     9241 aagaaaataa ggttgttgct tctcttcagc cagcacgtca tgccaggtta attcgttagc
     9301 catctcgctc tcctgcgaat cttcaatccg cctagcttaa ctgcttcttt ctacagaaca
     9361 aaatcatgct cattttttag acttaaacga gtgcaaaaaa atgtaaattg gtaaaatttc
     9421 acacccgcat aagttgatgt aaaacaataa aatccattta cgaccacaat cgatgtgtgg
     9481 tttttattga tttaaatcaa agaatcaagg gtggttgagg agtatatata cactcaagca
     9541 acaatggttt taccaattag ccgcgacagg ctgaacaaat caaataattt tgccggggag
     9601 gcatcacatg attacaggta tccagattac taaagccgct aacgacgatc tgctgaactc
     9661 tttctggctg ctggacagcg aaaaaggcga agcacgttgc atcgttgcaa aagcaggtta
     9721 tgcagaagat gaagtggttg cagtaagcaa actgggtgac attgaatacc gtgaagttcc
     9781 agtagaagtg aaaccagaag ttcgcgttga aggtggtcaa cacctgaacg ttaacgttct
     9841 gcgtcgtgaa actctggaag atgcagttaa gcatccggaa aaatatccgc agctgaccat
     9901 ccgtgtatcc ggttatgcag ttcgctttaa ctctctgact ccggaacagc agcgcgacgt
     9961 tatcgctcgt acctttactg aaagcctgta aggcttaagt agcaagttac taagcggaag
    10021 agggcgacct cttccgcttt ttttcgttaa tagaaaatgc gtaccgcgca atagaccagc
    10081 aacagggcaa gcacgatatt taactggcga ccatactggc gaaacaatcg ctgaaacaga
    10141 tgccccgcca gcgcccagca cacattgcca aacgtcccaa tcatcgccag caaaacgctg
    10201 acgccaacta cccagcttag cgcctgtgtt tgcggcagaa caaacgtcga cagtgccgta
    10261 acaccgtaca aaatgatttt gacgttcaca aactgcaaag caaagctggc ccaaaagctg
    10321 attggttttg cctgaagtcc gtcttccttt gttgggctgg tggcgatttt ccacgccagc
    10381 cagacaatat atgccgcccc cgcccaactc aaaaggtgta ccgctgccgg gtcaatcact
    10441 gccagtgaaa atgaaatgcc cgcacacagt aacatcacaa tcaaaaatcc cagactcatc
    10501 cctgccagca cgcgggtact ttgacgaaat ccatgcgacg tagcagagct aagggcgaga
    10561 atattgttcg gtcttggcgt catagcggta atcagggtgt aagtccaaaa agcacttaaa
    10621 agggtcggtg tcacttgtta tctcctctgt tttggtagac a
//
LOCUS       DAJTMC010000144        10445 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.144, whole genome shotgun sequence.
ACCESSION   DAJTMC010000144 DAJTMC010000000
VERSION     DAJTMC010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10445
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.144"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(9..1907)
                     /gene="espL1"
                     /locus_tag="OO130_003268"
     CDS             complement(9..1907)
                     /gene="espL1"
                     /locus_tag="OO130_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
                     /protein_id="HCR5366555.1"
                     /translation="MSQNDIIIRTHYKSPHRLHIDSDIPTPSSELINQFARQLITLLD
                     TSDLSSMLSYCITQEFTANCRKISQNCYSTALFIINFATSPIHAENILITLHYKKEII
                     PLFLETTPIKANHLRSILDYIEQEQLTAEDRNHCMKLSKKIHREKTIQPTVNLNGSAC
                     FLQSPSDAIFCRHLSLQYALDSLRNGKGKVNLIKHYSSVESIQQHVPLVRDAEFRALL
                     RHPPAGSRVIASKDFGFALDIFFCRMMANNVSHMSAILYIDNHTLSVRLRIKQLVYGQ
                     LNYVVSVYDPNDTNVAVRGTHRTARGFLSLDKFISSGPDAQTWADRYVRNCAIAILPL
                     LPVGVPGAIFAGIASRMPFAPIHPSAMLLIMATGQTQQLITLFKQLPILPEKEIIEII
                     TAQNSVGTPALFLAMMNGHTDNVKIFMQEIQSLIYNHIIHEDNLVKLLQTKSANETPG
                     LYISMLYGFDETIDIFLNALTTPIAQELLNKKIVMDILAMKTRDGELGLFAAMENNHP
                     LCVTRFLSKINGIAFKYKLSKANIMDLLKGATAHGTPVLYIAMSKGNEDVVLSYISTL
                     NIFAKKYSFSQRQLFTLLAAKNHDNMSAVHIAIHHNHYKTVETYYAAINVISQSLSFS
                     ADELKTYL"
     gene            2429..4357
                     /gene="thrS"
                     /locus_tag="OO130_003269"
     CDS             2429..4357
                     /gene="thrS"
                     /locus_tag="OO130_003269"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HCR5366556.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHAARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            4361..4903
                     /gene="infC"
                     /locus_tag="OO130_003270"
     CDS             4361..4903
                     /gene="infC"
                     /locus_tag="OO130_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HCR5366557.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQRVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            5000..5197
                     /gene="rpmI"
                     /locus_tag="OO130_003271"
     CDS             5000..5197
                     /gene="rpmI"
                     /locus_tag="OO130_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HCR5366558.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            5250..5606
                     /gene="rplT"
                     /locus_tag="OO130_003272"
     CDS             5250..5606
                     /gene="rplT"
                     /locus_tag="OO130_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HCR5366559.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     misc_feature    5723..5847
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            5729..5773
                     /gene="pheM"
                     /locus_tag="OO130_003273"
     CDS             5729..5773
                     /gene="pheM"
                     /locus_tag="OO130_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HCR5366560.1"
                     /translation="MNAAIFRFFFYFST"
     gene            6056..7039
                     /gene="pheS"
                     /locus_tag="OO130_003274"
     CDS             6056..7039
                     /gene="pheS"
                     /locus_tag="OO130_003274"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HCR5366561.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DTTRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            7054..9441
                     /gene="pheT"
                     /locus_tag="OO130_003275"
     CDS             7054..9441
                     /gene="pheT"
                     /locus_tag="OO130_003275"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HCR5366562.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDCLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            9446..9745
                     /gene="ihfA"
                     /locus_tag="OO130_003276"
     CDS             9446..9745
                     /gene="ihfA"
                     /locus_tag="OO130_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HCR5366563.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            complement(10049..10189)
                     /locus_tag="OO130_003277"
     CDS             complement(10049..10189)
                     /locus_tag="OO130_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HCR5366564.1"
                     /translation="MSQKSLTAITFCVTAILIIWMLHGSLCEIRMSFWGAEFAAFLQC
                     KQ"
BASE COUNT         2744 a         2364 c         2664 g         2673 t
ORIGIN      
        1 atagttggtt ataaatacgt ctttagttca tcagcactaa aactcaggct ttggctgatt
       61 acatttatag cagcataata tgtttctaca gttttataat gattatgatg aatggctata
      121 tgaacagctg acatgttgtc atgattttta gcggccaaca gtgtgaataa ctgacgttga
      181 ctaaaagaat atttttttgc aaaaatattc agcgtcgata tataagataa cacgacgtct
      241 tcattaccct tgctcatggc gatgtataat acaggggttc catgtgctgt agcgcctttt
      301 aataaatcca tgatgttagc tttgctcaac ttgtatttaa aggcgatgcc attaatttta
      361 gagaggaacc gtgtgacaca caaagggtga ttattttcca ttgcagcaaa caaccctagt
      421 tcaccatcac gtgttttcat tgctaaaata tccatcacta tttttttgtt taaaagctct
      481 tgtgctattg gagtggttaa tgcattcaga aagatatcga ttgtttcatc gaacccatac
      541 aacatggaga tatagagccc aggtgtttcg ttagcacttt tagtttgcag taatttaacc
      601 agattatctt catgaatgat atgattgtat atcagtgact gaatttcttg cataaatatt
      661 tttacgttgt cagtatgtcc gttcatcata gccagaaata aagcaggtgt accaacgcta
      721 ttctgcgcag ttattatttc aatgatttct ttttcaggga gtatgggcaa ctgtttgaat
      781 aatgtaataa gctgttgagt ctggcctgtg gccattatta acaacattgc cgatggatgt
      841 ataggggcaa atggcattcg tgatgcaata cccgcgaaaa tagcccctgg aactcccaca
      901 ggtaataggg gcagaatagc aattgcacag ttgcgaacat acctatcagc ccaggtctga
      961 gcatcgggac ctgaactgat gaacttatca agcgaaagaa agccccgtgc tgtcctgtgg
     1021 gtgcctctga cggcaacgtt ggtatcgttc gggtcgtaga cggacactac atagtttaat
     1081 tgtccataca ctaactgctt tattcgtagc cttactgaca aagtatgatt gtctatatat
     1141 aaaatcgcgg acatatgact gacattgttt gccatcattc gacaaaagaa aatatctaaa
     1201 gcgaagccaa aatccttact cgcgataacg cgactccctg caggaggatg gcgaagtaat
     1261 gctctgaact ccgcatcccg gactaagggg acatgctgct gtatggattc aacggaggag
     1321 taatgtttaa tcaggttgac tttgcctttt ccatttctca atgaatcaag ggcgtattgc
     1381 aatgacagat ggcgacaaaa aatagcgtca gaaggagatt gcaaaaaaca tgcactacca
     1441 ttgagattta ctgttggttg tatagttttt tctctatgga tttttttaga cagtttcata
     1501 caatggttac gatcttcggc agttaactgt tcctgctcaa tataatccag tatgcttcgc
     1561 aaatggttag ccttaatagg cgtggtttcc agaaataagg gaatgatttc ttttttatag
     1621 tgtaatgtaa taagtatatt ttctgcatgg atgggtgaag tggcaaagtt aatgataaaa
     1681 agggcagtgg agtaacaatt ttgtgatatt tttcgacagt ttgcggtaaa ttcctgagta
     1741 atacagtatg acagcatcga acttaagtca gaggtatcaa gtagggtgat gagttggcgc
     1801 gcaaattgat taataagctc tgatgaaggc gttggtatgt cgctatcgat gtgcaatcta
     1861 tgaggagact tataatgagt tctgataatg atatcgtttt gactcataat ctattcacct
     1921 tatatatact ggaggttttg aggatgatga ttaattatta tcgccagaac gaaatgtgca
     1981 cgaaaaattg ctataagaat tgtgtcactt ttatcattgt gatatgacca cttcgtcatt
     2041 aatctcatga tcgtttaaat actaccgcca aatcacgcaa cgaactttgc tggattcagt
     2101 ttatgctgct gtaaatccgc tcgcagtaaa cctttcagac gcaccgtgat gttatcagtg
     2161 gttctttaag cgttttgctg gtgtactcac tacaaacgaa ttgcgaatcg aatcaatgtg
     2221 aaacggaaag gtacaatctc ccttttttta gtgttaacat cgttcaaccg ggtggaaggt
     2281 gagaggttaa tgtctttcat ttgtgtacct gattgcgaac caatttagca tttgttggct
     2341 agatggtttc gcaatgaact gttaataaac aaatttttct ttgtatgtga tctttcgtgt
     2401 gggtcaccac tgcaaataag gatataaaat gcctgttata actcttcctg atggcagcca
     2461 acgccattac gaccacgctg taagccccat ggatgttgcg ctggacattg gtccaggtct
     2521 ggcgaaagcc tgtatcgcag ggcgcgttaa tggcgaactg gttgatgctt gcgatctgat
     2581 tgaaaacgac gcacaactgt cgatcattac cgccaaagac gaagaaggtc tggagatcat
     2641 tcgtcactcc tgtgcgcacc tgttagggca tgcgattaaa caactttggc cgcataccaa
     2701 aatggcaatc ggcccggtta ttgacaacgg tttttattac gacgttgatc ttgaccgcac
     2761 gttaacccag gaagatgtcg aagcactcga gaagcggatg catgagcttg ctgagaaaaa
     2821 ctacgacgtc attaagaaga aagtcagctg gcacgcagcg cgtgaaactt tcgccaaccg
     2881 tggggagagc tacaaagtct ccattcttga cgaaaacatc gcccatgatg acaagccagg
     2941 tctgtacttc catgaagaat atgtcgatat gtgccgcggt ccgcacgtac cgaacatgcg
     3001 tttctgccat catttcaaac taatgaaaac ggcaggggct tactggcgtg gcgacagcaa
     3061 caacaaaatg ttgcaacgta tttacggtac ggcgtgggca gacaaaaaag cacttaacgc
     3121 ttacctgcag cgcctggaag aagccgcgaa acgcgaccac cgtaaaatcg gtaaacagct
     3181 cgacctgtac catatgcagg aagaagcgcc gggtatggta ttctggcaca acgacggctg
     3241 gaccatcttc cgtgaactgg aagtgtttgt tcgttctaaa ctgaaagagt accagtatca
     3301 ggaagttaaa ggtccgttca tgatggaccg tgtcctgtgg gaaaaaaccg gtcactggga
     3361 caactacaaa gatgcaatgt tcaccacatc ttctgagaac cgtgaatact gcattaagcc
     3421 gatgaactgc ccgggtcacg tacaaatttt caaccagggg ctgaagtctt atcgcgatct
     3481 gccgctgcgt atggccgagt ttggtagctg ccaccgtaac gagccgtcag gttcgctgca
     3541 tggcctgatg cgcgtgcgtg gatttaccca agatgacgcg catatcttct gtactgaaga
     3601 acaaattcgc gatgaagtta acggatgtat ccgtttagtc tatgatatgt acagcacttt
     3661 tggcttcgag aagatcgtcg tcaaactctc cactcgtcct gaaaaacgta ttggcagcga
     3721 cgaaatgtgg gatcgtgctg aggcggacct ggcggttgcg ctggaagaaa acaacatccc
     3781 gtttgaatat caactgggtg aaggcgcttt ctacggtccg aaaattgaat ttaccctgta
     3841 tgactgcctc gatcgtgcat ggcagtgcgg tacagtacag ctggacttct ccttgccgtc
     3901 tcgtctgagc gcctcctatg tgggcgaaga caacgagcgt aaggtaccgg taatgattca
     3961 ccgcgcaatt ctggggtcga tggaacgttt catcggtatc ctgaccgaag aattcgctgg
     4021 tttcttcccg acctggcttg cgccagttca ggttgttatc atgaatatta ccgattcaca
     4081 gtctgaatac gttaacgaat tgacgcaaaa actatcaaat gcgggcattc gtgttaaagc
     4141 agacttgaga aatgagaaga ttggctttaa aatccgcgag cacactttgc gtcgcgtccc
     4201 atatatgctg gtctgtggtg ataaagaggt ggaatcaggc aaagttgccg ttcgcacccg
     4261 ccgtggtaaa gacctgggaa gcatggacgt aaatgaagtg atcgagaagt tgcaacaaga
     4321 gattcgcagc cgcagtctta aacaattgga ggaataaggt attaaaggcg gaaaacgagt
     4381 tcaaacggcg cgccctaacc gtatcaatgg cgaaattcgc gcccaggaag ttcgcttaac
     4441 aggtctggaa ggcgagcagc ttggtattgt gagtctgaga gaagctctgg agaaagcaga
     4501 agaagccgga gtagacttag tcgagatcag ccctaacgcc gagccgccgg tttgtcgtat
     4561 aatggattac ggcaaattcc tctatgaaaa gagcaagtct tctaaggaac agaagaaaaa
     4621 gcaaagagtt atccaggtta aggaaattaa attccgtcct ggtacagatg aaggcgacta
     4681 tcaggtaaaa ctccgcagcc tgattcgctt tctcgaagag ggtgataaag ccaaaatcac
     4741 gctgcgtttc cgcggtcgtg agatggcgca ccagcaaatc ggtatggaag tgcttaatcg
     4801 cgtgaaagac gatttgcaag aactggcagt ggtcgaatcc ttcccaacga agatcgaagg
     4861 ccgccagatg atcatggtgc tcgctcctaa gaagaaacag taaggccttc aagtagcact
     4921 gtctgtggag ccttcgggct tcacaggctt tgttcgcctt tgtttcgttt attaacaatg
     4981 cgaagtggaa gttattaaaa tgccaaaaat taagaccgta cgcggtgctg ctaagcgctt
     5041 caaaaaaacc ggtaaaggtg gttttaagca caagcacgct aacctgcgtc acattctgac
     5101 caaaaaagcg accaaacgta aacgtcacct gcgtccgaaa gccatggttt ccaaaggcga
     5161 tctgggcctg gtaatcgcgt gcctgccgta cgcataagcc gttaacgttt ttaacttttt
     5221 aattagaata tagatacagg agagcacata tggctcgcgt aaaacgtggt gttattgcac
     5281 gcgcacgtca caagaaaatt ttgaaacaag ctaaaggcta ctacggtgcg cgttctcgcg
     5341 tataccgcgt tgccttccag gctgttatca aagctggtca gtatgcttac cgtgaccgtc
     5401 gtcaacgtaa gcgtcagttc cgtcaactgt ggattgcacg tatcaacgca gcagcacgtc
     5461 agaacggtat ttcttacagc aaattcatca atggcctgaa aaaagcctct gttgaaatcg
     5521 accgtaagat cctggctgat atcgcagtat tcgacaaagt agcgttcacc gctctggttg
     5581 aaaaagcgaa agcagctctg gcataagcca gttgaaagag ggagcttgtc tccctctttt
     5641 cgtttcaacg ccatcaaaac attgactttt atcgccgtag ccttttcaat aaaggtcttt
     5701 tgaagagtac caaaaggtaa cgcaagcaat gaatgctgct attttccgct tcttttttta
     5761 ctttagcacc tgaatccagg aggctagcgc gtgagaagag aaacggaaaa cagcgcctga
     5821 aagcctccca gtggaggctt tttttgtatg cgcgttttga aaaataaaaa gccagtctat
     5881 caggcgtaat tgtcgcagcc agtctggata cggccagcgc ggagaaaccg gagcgtattt
     5941 aagtacgtga gaatttcgag cactgcccgg gaccaaaatg gcaagtaaaa tagcctgatg
     6001 ggataggctc taagtccaac gaaccagtgt caccactgac acaatgagga aaaccatgtc
     6061 acatctcgca gaactggttg ccagtgcgaa ggcggccatt agccaggcgt cagatgttgc
     6121 cgcgttagat aatgtgcgcg tcgaatattt gggtaaaaaa gggcacttaa cccttcagat
     6181 gacgaccctg cgtgagctgc cgccagaaga gcgtccggca gctggtgcgg ttatcaacga
     6241 agcgaaagag caggttcagc aggcgctgaa tgcgcgtaaa gcggaactgg aaagcgcagc
     6301 gctgaatgcg cgtctggcgg cggaaacgat tgatgtctcc ctgccaggtc gtcgcattga
     6361 aaacggcggt ctgcatccgg ttacccgtac catcgaccgt atcgaaagtt tcttcggtga
     6421 gcttggcttt accgtggcaa ccgggccgga aatcgaagac gattatcata acttcgatgc
     6481 tctgaacatt cctggtcacc acccggcgcg cgctgaccac gacactttct ggtttgacac
     6541 tacccgcctg ctgcgtaccc agacctctgg cgtacagatc cgcaccatga aagcccagca
     6601 gccaccgatt cgtatcatcg cgcctggccg cgtttatcgt aacgactacg accagactca
     6661 cacgccgatg ttccatcaga tggaaggtct gattgttgat accaacatca gctttaccaa
     6721 cctgaaaggc actctgcacg atttcctgcg taacttcttt gaggaagatt tgcagattcg
     6781 cttccgtcct tcctacttcc cgttcaccga gccttctgca gaagttgacg tcatgggtaa
     6841 aaacggtaaa tggctggaag tgctgggttg cgggatggtg catccgaacg tgctgcgtaa
     6901 cgttggcatc gacccggaag tttactctgg tttcgccttc gggatgggga tggagcgtct
     6961 gactatgttg cgttacggcg tcaccgacct gcgttcattc ttcgaaaacg atctgcgttt
     7021 cctcaaacag tttaaataag gcaggaatag attatgaaat tcagtgaact gtggttacgc
     7081 gaatgggtga acccggcgat tgatagcgat gcgctggcga atcaaatcac tatggcgggc
     7141 ctggaagttg acggtgtaga accggttgcc ggtagcttcc acggcgtggt cgttggtgaa
     7201 gtggttgagt gtgcgcagca tccgaacgct gacaaactgc gtgtgacaaa agtgaatgtc
     7261 ggcggcgatc gcctgctgga catcgtctgt ggtgcgccaa actgccgtca gggcctgcgt
     7321 gtggcggtag ctaccattgg tgctgttctg ccgggtgatt tcaaaattaa agcggcgaaa
     7381 ctgcgtggcg aaccgtctga agggatgctg tgctccttct ctgaactggg aatttctgac
     7441 gatcacagcg gcattatcga actgcctgca gatgcgccga ttggcactga catccgcgaa
     7501 tacctgaaac tcgatgacaa caccatcgaa atcagcgtaa cgccaaaccg tgccgactgc
     7561 ttaggtatca ttggtgttgc gcgtgacgtt gccgtgctga atcagctgcc gctggttgaa
     7621 ccggaaatcg ttccggttgg tgcgaccatc gacgacacgt tgccgattac agtcgaagcg
     7681 cctgaagcct gcccgcgcta tcttggccgt gtggtaaaag gcattaacgt taaagcgcca
     7741 actccgctgt ggatgaaaga aaaactgcgt cgttgcggga tccgttctat cgatgcagtt
     7801 gttgacgtca ccaactatgt actgctcgaa ctgggccagc cgatgcacgc tttcgataaa
     7861 gatcgcattg aaggcggcat tgtggtgcgg atggcaaaag agggcgaaac gctggtgctg
     7921 cttgatggca ctgaagcgaa gctgaatgct gacactctgg tcatcgccga ccacaacaag
     7981 gcgctggcga tgggcggcat ctttggtggc gaacactctg gcgtgaatga cgaaacgcaa
     8041 aacgtcttgc tggaatgcgc tttcttcagc ccgctgtcta tcaccggtcg tgctcgtcgt
     8101 catggcctgc atactgatgc gtctcaccgt tatgagcgtg gcgttgatcc ggcactgcag
     8161 cacaaagcga tggaacgtgc gacccgtctg ctgatcgaca tctgcggtgg tgaggctggt
     8221 ccggtaattg atatcaccaa cgaagcaacg ctgccgaagc gtgcaaccat cactttacgt
     8281 cgtagcaaac tggattgcct gatcggccat catattgctg atgaacaggt aactgacatt
     8341 ctgcgtcgtc tcggctgcga agtgaccgaa ggcaaagacg agtggcaggc agttgcgccg
     8401 agctggcgtt tcgatatgga gattgaagaa gatctggtcg aagaagtcgc gcgtgtttac
     8461 ggctacaaca acatcccgga tgagccggta caggcaagcc tgattatggg tactcaccgt
     8521 gaagctgacc tgtcgctcaa gcgcgtgaaa acgctgctca acgacaaagg ctatcaggaa
     8581 gtgatcacct acagcttcgt tgatccgaaa gtgcagcaga tgatccatcc aggcgttgaa
     8641 gccttactgc tgccaagccc gatctctgtt gaaatgtcag caatgcgtct ttctctgtgg
     8701 accggcctgc tggcaaccgt ggtgtacaac cagaaccgtc agcagaaccg tgtgcgcatt
     8761 ttcgaaagcg gtctgcgttt cgtaccagat actcaggcac cgttgggcat tcgtcaggat
     8821 ctgatgttag ccggtgtgat ttgcggtaac cgttacgaag agcactggaa cctggcaaaa
     8881 gagaccgttg atttctatga tttgaaaggc gatcttgaat ccgttctcga cctgaccggt
     8941 aaactgaatg aggttgagtt ccgtgcagaa gcgaatccgg cactgcatcc ggggcaatcc
     9001 gcagcgattt atctgaaagg ggaacgtatt ggttttgttg gagttgttca tcctgaactg
     9061 gaacgtaaac tggatcttaa cggtcgcact ctggtgttcg aactggagtg gaacaagctc
     9121 gcagaccgcg tggtgcctca ggcgcgcgag atttctcgct tcccggcgaa ccgtcgtgac
     9181 atcgcggtgg tggtcgcaga aaacgttccc gcagcggata ttttatccga atgtaagaaa
     9241 gttggcgtaa atcaggtagt tggcgtaaac ttatttgacg tgtaccgcgg taagggtgtt
     9301 gcggaggggt ataagagcct cgccataagc ctgatcctgc aagataccag ccgtacactc
     9361 gaagaagagg agattgccgc taccgtcgcc aaatgtgtag aggcattaaa agagcgattc
     9421 caggcatcat tgagggattg aacctatggc gcttacaaaa gctgaaatgt cagaatatct
     9481 gtttgataag cttgggctta gcaagcggga tgccaaagaa ctggttgaac tgtttttcga
     9541 agagatccgt cgcgctctgg aaaacggcga acaggtgaaa ctctctggtt ttggtaactt
     9601 cgatctgcgt gataagaatc aacgcccggg acgtaacccg aaaacgggcg aggatattcc
     9661 cattacagca cggcgcgtgg tgaccttcag acccgggcag aagttaaaaa gccgggtcga
     9721 aaacgcttcg cccaaagacg agtaatcaga tctaactaaa aaggccgctc tgcggccttt
     9781 tttctttcaa taactaattg atttataatg ttttatatct cttcgttcca cgatgcgtcc
     9841 acatttagtg gggggagggg agtaagcccg ctacattgtg ggctttgtct tatagtctga
     9901 tcactgtttc aagtcggtga ggacaccaaa aagaaccccc gcagttttta cgctgcgggt
     9961 ttgttgttca tgtctgtgag atagggtgcc ttatcgacct taccctggca accgattgac
    10021 gggggattgc tcccccgtcg cggtttcctt actgcttaca ctgtaagaac gccgcaaact
    10081 ccgctcccca gaagctcatc cgtatttcgc acagcgaacc gtgcagcatc cagatgatga
    10141 ggattgccgt cacgcagaac gtgatggccg taagcgattt ttgcgacata gcgcttgctc
    10201 ctttttcggg gaggcgctaa cctatcactt gcttagggta gacggttagg gcctcggtta
    10261 aacaaaaatg ttttccgggg cctttccaca tctggccttc aggtattccc tccgaccatc
    10321 agccgaaagg cacccgcgcg taatctatcg cttttttgtt actccggcaa ttctgctcgt
    10381 taattctgag gtaagggcaa actcatctga ttgtttcctc tgtgtgaagt tggcagttca
    10441 tgcca
//
LOCUS       DAJTMC010000145        10370 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.145, whole genome shotgun sequence.
ACCESSION   DAJTMC010000145 DAJTMC010000000
VERSION     DAJTMC010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10370
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.145"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(37..1032)
                     /gene="gapA"
                     /locus_tag="OO130_003278"
     CDS             complement(37..1032)
                     /gene="gapA"
                     /locus_tag="OO130_003278"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCR5366565.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            1374..1787
                     /gene="msrB"
                     /locus_tag="OO130_003279"
     CDS             1374..1787
                     /gene="msrB"
                     /locus_tag="OO130_003279"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HCR5366566.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            1829..2101
                     /locus_tag="OO130_003280"
     CDS             1829..2101
                     /locus_tag="OO130_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HCR5366567.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            complement(2262..2903)
                     /gene="pncA"
                     /locus_tag="OO130_003281"
     CDS             complement(2262..2903)
                     /gene="pncA"
                     /locus_tag="OO130_003281"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HCR5366568.1"
                     /translation="MPHRALLLVDLQNDFCAGGALAVPEGDSTVEVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTSLDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(2914..3930)
                     /gene="ansA"
                     /locus_tag="OO130_003282"
     CDS             complement(2914..3930)
                     /gene="ansA"
                     /locus_tag="OO130_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HCR5366569.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPDDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(4097..5953)
                     /gene="sppA"
                     /locus_tag="OO130_003283"
     CDS             complement(4097..5953)
                     /gene="sppA"
                     /locus_tag="OO130_003283"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HCR5366570.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDHNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPSAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            6114..6665
                     /locus_tag="OO130_003284"
     CDS             6114..6665
                     /locus_tag="OO130_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HCR5366571.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAILEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            6782..7825
                     /gene="selD"
                     /locus_tag="OO130_003285"
     CDS             6782..7825
                     /gene="selD"
                     /locus_tag="OO130_003285"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HCR5366572.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQACVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKATAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            7830..9791
                     /gene="topB"
                     /locus_tag="OO130_003286"
     CDS             7830..9791
                     /gene="topB"
                     /locus_tag="OO130_003286"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCR5366573.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEVGSGAIA"
     gene            9918..>10370
                     /locus_tag="OO130_003287"
     CDS             9918..>10370
                     /locus_tag="OO130_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416276.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5366574.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWEIFNSNFERMIKENKAMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLITPYRFYHKQWSETLEFWPRKPEPGKDTFQYH"
BASE COUNT         2612 a         2533 c         2735 g         2490 t
ORIGIN      
        1 gaagtcgctc tttttagatc acagtttcat ctcaacttat ttggagatgt gagcgatcag
       61 gtccagaact ttgttggagt aaccggtttc gttgtcgtac caggatacca gtttcacgaa
      121 gttgtcgttc agagcgatac cagctttagc atcgaacacg gaagtgcaaa cttcgccgtt
      181 gaaatcggta gatactacgt catcttcggt gtagcccaga acgcctttca tttcgccttc
      241 agcagcggct ttaacggcag ctttgatctg ctcgtaagtt gcagcttttt ccagacgaac
      301 ggtcaggtca actacggata cgttcggggt cggaacgcgg aacgccatac cagtcagttt
      361 gccattcagt tctggcagta ctttacctac agctttagca gcaccggtag aggacgggat
      421 gatgttctgg gaagcgccgc ggccgccgcg ccagtctttg tgagacgggc catcaacggt
      481 tttctgagta gcggtagtag cgtgaacggt ggtcatcaga ccttcgatga tgccgaagtt
      541 atcgttgata actttagcca gcggagccag gcagttggtg gtgcaggaag cgttggaaac
      601 gatgtcctgg ccagcatatt tgtcgaagtt agcgccttta acgaacatcg gagtgttgtc
      661 tttagacgga ccagtcataa ccactttctt cgcaccagcg gtgatgtgtt tacgagcagt
      721 ttcgtcagtc aggaacagac cagttgcttc agcgacaacg tcaacaccaa cttcgtccca
      781 tttcaggtta gccggatcac gttcagcggt aacacggatt tttttaccgt taacgatcag
      841 atgaccgtct ttcacttcaa cggtaccgtc gaaacggccg tgagtggagt catatttcag
      901 catgtatgcc atgtaatcag cgtctaacag gtcgttgatt gcaacgatct cgatgtcaga
      961 acgtttctga gcagcacgga aaacaatgcg accgatacgg ccaaaaccgt tgatacctac
     1021 tttgatagtc atatattcca ccagctattt gttagtgaat aaaaggttgc ctgtaaaatt
     1081 acaaaaacct tacgcagcgt caagcggaat cgtgtcaatc attgcgacaa atcaatcctg
     1141 tgcctaagca ttacgcgact gactcgcctc actcttcctt tgggcttgaa accacatggg
     1201 gtcggcgccc cgaattttaa agggcaatta cgataaaaat gtgattttca tcacgattta
     1261 ggtgcagcca tttcgtcgag atcaagttta ggacaaaatt ttgccagttg gtgttaatgt
     1321 tttgttagaa tcagtcacgt aatgtgagca cgattaaagt gagatgtgag caaatggcta
     1381 ataaaccttc ggcagaagaa ctgaaaaaaa atttgtccga gatgcagttt tacgtgacgc
     1441 agaatcatgg gacagagccg ccatttacgg ggcgtttact gcataacaag cgtgacggcg
     1501 tatatcactg tttgatctgc gatgccccgc tgtttcattc cgaaaccaag tatgattccg
     1561 gctgtggctg gcccagtttc tacgaaccgg tgagtgaaga atccattcgt tatatcaaag
     1621 acttgtcaca tggaatgcag cgcatagaaa ttcgttgcgg taactgtgat gcccatctgg
     1681 ggcatgtctt tcccgacggg ccgcaaccaa caggcgaacg ttattgtgtt aactctgctt
     1741 ctttacgctt cactgatggc gaaaacggcg aagaaatcaa cggttgaaaa aacgattcag
     1801 caacttattc cacaggagcg gaaaaattat gaatcttgat gacattatca acagcatgac
     1861 gcctgaggta taccagcgtt tgtcgaccgc cgttgaactg gggaaatggc ctgatggcgt
     1921 tgcgttgacc gaggaacaaa aagagaattg cctgcaactg gtgatgctgt ggcaagcccg
     1981 ccataatacc gaagcacagc atatgacaat tgacacaaat ggtcagatgg tcatgaagag
     2041 caaacagcag ttaaaagaag attttggtat ctcagcaaag cctattgcta tgtttaagtg
     2101 aatgagttat ctagtattta agcctgttat taagggaggg caaatatact tgttcggacg
     2161 tcgtgtttta gctgcaagta ggttaaatgc aacactgaca agtattatct ggcctacaat
     2221 gtcattgagg aaatgtaggc cagagatgaa ttcaactaat attacccctg tgtctcttcc
     2281 cagtctgcca gcgtatatag cgttgcccca gctgctgaca tctccataaa cgcgtgtgca
     2341 ctgtcctggg gctggatatt cacgccacga caaccatcgg taatcacgtt caccttataa
     2401 cctaactgta acgcgtccag cacggtaaac ttcacgcaat agtcagtagc caggcccata
     2461 acgatcaatt catcgatttc atgatcgcgt aaccagtcat cgagagaggt tttctgccga
     2521 cggccgttat caaaaaaggc actgtaactg tcaactaaag gattttcgcc tttatggaac
     2581 accgctgcga tcgctttttg gttcagtaac ggatgtaatt gtgcgccttc actgttctgc
     2641 acacagtgat ctggccagaa ggtttgtggc aaaccgtcga gttggcctgg cgtataaggc
     2701 tctacaccgt gctgactggc aaaactgccg tgattcgccg gatgccagtc ctggctggcg
     2761 ataaccgctt ctccgcgcga ctggcaccag tcaataaggc ggttagcaac ctccaccgta
     2821 ctgtcacctt ccggtacggc gagcgcgcca ccagcacaga aatcattttg taaatcgacc
     2881 agtaacaggg cgcgatgggg cattgcagtc tccttaatca tccggcgtca gttcgccgcg
     2941 caggttttgg ctcatggcct tgcgaatggt ttcagtatcc agttcctggc tcagcaggta
     3001 atgcagtttg gttagcgtgg cttctacagt catatctgca ccgccaataa cgccggcatg
     3061 ggcgagggcg ttaccggtgg cataaccacc catattcact ttaccggaca tacattgtgt
     3121 caggttgacc accacaatac cgcgatcgct ggcttcttgt aattcctgca ggaaggcttt
     3181 gttttgtggc gcattaccca cgccatagga gcgcagaatc aatgctttca ctggttggcg
     3241 cagaaaattg cgcacgacat cagcagaaat ccccggataa atcgtcacta cgccaattgg
     3301 ttgtggcgtg attggatgaa cgattaattc cccttcaccg tgcggggcgg gtggcgtatt
     3361 caaacgacga atatggatac cagcttccag taacggagga aggtttggag aggcaaacgc
     3421 atcaaaacca tcggcatggg ctttggtagt gcggttgccg cgatacaatc ggttattgaa
     3481 aaagagcgtt acttcgttga tcggataatt cgccgcaacg tacaacgcat tcagcagatt
     3541 aatttgtccg tcagagcgta actcagccag cgggatttgt gaccctgtca caatgaccgg
     3601 tttaccgaga ttctcgagca tgaacgacag cgcagaggcg gtatacgcca tcgtgtcggt
     3661 accgtgtaga atgacaaaac catcatagtc gtcatagtgc gctttaatat cttcagcaat
     3721 atgctgccag tcatccggcg tcatatcaga agaatccatc aacggcgtat attcatgaat
     3781 ggtgaaatct ggcatctccg ggcgatggaa ttccggcatc agcgccagtt ggcgttgtag
     3841 atgacctgac accggaatat aaccctgctc ggaacgctgc atcccgatgg tcccgcccgt
     3901 gtaggcgacg taaattgatt tcttttgcat gatattgata tgaatgatat cgaaagagca
     3961 aaagtatata cgtgaggcag gaggagcgca cctgatacta tgcggataag gaaatatggg
     4021 gcgagacagc gggaagagga ttcgtcgagg taaaaaataa tgtggccgac gatctgtcgg
     4081 ccactcagta caagacttaa cgcacgttgg cgcaggtcag gcaaaacgca taacggtttt
     4141 gtgggtcgtt aaacgcggcc agcttgtcgc tttcactttt aacggtagag gctaccgagg
     4201 caagcggtgc aggtaacatg gcctggaacg catctggcaa cattgcccgg acagaaccag
     4261 acatgttgtc catcactttg tcgaagaagg tcggttcatc aacgtagtat tccagatgcc
     4321 actgtttcac ttttgcgagc tctgctgctt tggcaaccgc atcatcgaaa tccccgagac
     4381 tatcgaccag cccgttagct tttgcatcct gaccggtcca gacgtggccc tgggcgattt
     4441 tatcaatttg ctccggcgtc gaatgacgcg catcagcaac cagcgtgata aagcgtttat
     4501 agccattctc aatgcttaat tgcatcatct gctgcgcttc cggcggcagt gccctggtga
     4561 tagaaacatc cgccagcggt gaagttgaga caccatcagt atgaacacca atcgaatcca
     4621 gactattttc tacggtggtg atcacgccga agataccgat agaaccggtc agggtgctgg
     4681 ggttagccac aatgtaatta gctggcgtgg aaatccagta accaccagat gccgccatgc
     4741 cgcccatcga tacaaccaca ggcttacccg ctgcccgggc tgctgccagt tcagcgcgaa
     4801 tcacttcaga cgcggtaacg ctgccgcctg ggctattaac acgcaggaca atcgctttca
     4861 ctttcgggtc aaggcgagcg tcgcggattt gtgccgcagt ggtatcaccg ccaacattcc
     4921 cctgagtttc ctcgccatcc ataattgcgc cattagcgaa gacgacaccg atgctgtcac
     4981 cggtatctgc cggcgttttc aatgcgtaat cgtaataact gatggcgcga taatttttat
     5041 cagtcttact ccagccgaac tctttggtca gagctttttc gatttcggca ctcgatgcca
     5101 gtgcatcgac cagcttgttt tccagtgcat atttcgcggt atcgccaccg gttttggtta
     5161 aaccctcaag caacccttgc gcgctaggga atacctgctg agcagggatc tgtcggttag
     5221 cggcaacagt attcagatag ttttgccaca gctcaccaat ccagcggctg tcagcttcgc
     5281 gggctgccgg tgacatatca tcacgaataa acggttcaac ggcagattta tacgtaccca
     5341 cgcggaacac atgggtggaa actttcagct tatccagcaa cgatttgtag tacagaccgt
     5401 tggtagcaaa accgtgcaga tcaaccacgc cttgcggtga cagccaaatt ttattggcga
     5461 aactggcgag ataatattgc ccctggctgt agttctcgcc aacggcataa accggtttcc
     5521 cgctgtcacg aaactctttc agggctttgc cgatgtactg catagacggc tggtcgccgc
     5581 ctgcgaagtt tttcagatcc atcacgatac cggtgatatt gtggtcgtcc ttcgcctggc
     5641 gaatggtgtt gacgatatca aacagtgagt tttcctgcag acgatcggaa ctggcaccaa
     5701 gcagctggcg gcttaattta ctaaaccgct gagaactgtc gggtttatcg acgatcacac
     5761 cggaaatgtc cagcagcagt gcgccacgac tggccgtttc tttcgaatca ccaccactga
     5821 cctgcatcca gatccccaca ccaaccagta cgaggaaaat aaagaacagg ttaagtacca
     5881 tttcacggac gaaattcagc agacgccacg tccatttaaa aaatccggca ataaatcgcc
     5941 aaagggttcg catgtattct cccaacttaa ggtcacacct gtcacaggcg caatagatgt
     6001 ggttatcgta atgacccaac cgacacttgt cagcaggaat tgcggactgt gctgtaacaa
     6061 aatccactct cgtgttaatt ttgtgaataa atatcacgac aggagttaat caaatggatg
     6121 cactcgaact attgatcaat cgccgtagcg cctcccgcct ggctgaaccc gcgccaacgg
     6181 gtgaacaact gcaaaacatc ctgcgtgcgg gtatgcgtgc gccggaccat aagtccatgc
     6241 aaccgtggca tttttttgtg attgaagggg aagggcgcga gcgtttcagc gccatactgg
     6301 aacagggggc gattgctgcc ggtagtgatg acaaagctat cgacaaagcc cgtaatgcgc
     6361 cgttccgcgc accgctcatc atcacggtgg tggcgaaatg cgaagaaaat cataaagtcc
     6421 cgcgatggga acaggaaatg tctgccggat gcgcggtcat ggcgatgcaa atggcagccg
     6481 ttgcccaggg atttggcggc atctggcgca gtggcgcatt aactgaaagt ccggtagtgc
     6541 gtgaagcatt cggttgccgt gagcaggata aaattgtcgg ttttctctac ctcggcacgc
     6601 cgcagttgaa agcatctacg tcgattaacg tcccggaccc gacgccgttt gtaacttatt
     6661 tctgataatt gtcgcgaaac tgtctggatt gtgagcaaag gcgcaggaat tcagacactc
     6721 tcacttatca cttcacggaa tgagggttac catacccgga ttgcattgac aggagatgtc
     6781 catgagcgag aactcgattc gtttgaccca atacagccac ggagctggtt gcggctgtaa
     6841 aatttcccca aaagtgttgg aaaccatcct gcacagtgag caggcgaagt ttgttgatcc
     6901 gaatttgctt gtgggtaatg agacccgcga cgatgcggcg gtgtacgatc tgggcaatgg
     6961 caccagcgtt atcagtacca ccgacttctt tatgccgatc gttgataatc ctttcgattt
     7021 tggccgcatt gcggcgacta acgccatcag cgatatcttc gcgatggggg gcaaaccgat
     7081 tatggcgatt gcgatcctcg gctggccgat taacaaactt tccccggaaa ttgcccgcga
     7141 agtaaccgaa ggtggacgct atgcatgccg tcaggcgggt attgcgctgg ctggcggtca
     7201 ctccatcgat gcgccggagc cgatttttgg tctggcggta acggggatcg taccgaccga
     7261 gcgggtgaag aaaaacagca ccgcacaagc cggatgcaaa ctgttcctga cgaaaccgct
     7321 ggggatcggc gttcttacca cggctgagaa aaaatcactg ctgaaaccag aacatcaggg
     7381 actagcgacg gaagtgatgt gccggatgaa catcgcaggc gcgtcctttg ccaacatcga
     7441 aggcgtaaaa gcgatgaccg acgttacggg ctttggtctg ctgggccatt tgagcgaaat
     7501 gtgtcagggc gcaggtgtgc aggcgtgcgt tgactatgac gcgatcccaa aattgccggg
     7561 ggttgaagag tacattaagt tgggcgcagt gcctggcggc accgaacgta actttgccag
     7621 ctacggtcat ctgatgggtg aaatgccgcg tgaagtgcgc gatttgctgt gcgatccgca
     7681 aacttctggc ggtttgctgc tggcggtcat gccggaagca gaaaatgagg tcaaagctac
     7741 agccgccgag tttggcattg aactgacggc aattggcgaa ctggtgccag cgcgcggcgg
     7801 tcgtgccatg gttgagattc gttaattcaa tgcggttgtt tattgccgaa aaaccgagtc
     7861 tggcgcgcgc cattgctgat gtcctgccca aaccgcaccg gaaaggcgat ggctttatcg
     7921 agtgcggtaa tggtcaggtg gtgacctggt gtatcggtca cctgcttgag caggcgcagc
     7981 cagacgccta cgacagccgc tatgcgcgct ggaatcttgc ggatttgccg attgtcccgg
     8041 aaaagtggca attacagccc cgaccctccg tgaccaaaca acttaacgtc atcaaacgct
     8101 tcctgcatga agccagcgaa atcgttcacg ccggggaccc ggatcgtgaa gggcaactgc
     8161 tggtggatga agtgctggac tatctgcaac tggcaccgga aaagcgccag caggtgcagc
     8221 gttgcttgat aaacgacctg aacccgcagg cggttgagcg ggcgatcgac cgtcttcggt
     8281 ccaacagtga gtttgtgcca ttgtgcgttt ctgcgctggc gcgagcgcgt gccgactggc
     8341 tgtacggcat caatatgacc cgtgcgtata ccattctcgg tcgcaatgcc ggttatcagg
     8401 gcgtactttc cgtgggacgc gtgcagacac ccgtgctcgg gctggtggtg cgccgcgatg
     8461 aagagattga aaacttcgtg gcgaaagact tctttgaagt caaagcacat atcgtgacac
     8521 ctgccgatga gcggtttacc gctatctggc aaccgagcga agcgtgtgaa ccgtaccagg
     8581 atgaagaagg gcgcttgtta catcgtccac tggcggagca tgtggttaac cgcattagtg
     8641 gtcaaccagc aattgtcacc agctataacg ataaacggga atcagaatcc gcgccgctgc
     8701 ctttttcgct ttcggcgttg cagattgaag cggcaaaacg ctttggtctg agcgcgcaga
     8761 acgtgcttga tatctgccag aagctgtacg aaacgcacaa gctaatcact tatccgcgtt
     8821 ccgattgtcg ctatttgcca gaagaacatt ttgccggacg ccacgcggtg atgaatgcca
     8881 tcagcgttca tgcaccagat ctgttgccgc aaccagtggt agatccagat atacgcaacc
     8941 gctgttggga tgacaaaaag gtcgatgcac accacgccat catcccgacc gcacggagtt
     9001 ctgcgatcaa cctgacggag aacgaagcga aggtctataa cctgattgcc cgtcagtact
     9061 tgatgcagtt ctgcccggat gcggtgttcc gcaagtgtgt tatcgaactg gacattgcca
     9121 aaggcaaatt tgtcgctaaa gcgcgttttc ttgctgaagc aggctggcgc acgctgttag
     9181 gcagcaaaga gcgcgatgaa gaaaacgacg gtacgccatt gcctgtggtg gcgaaaggcg
     9241 atgagttgct gtgtgaaaaa ggtgaagtgg tagagcggca aacccagccg ccgcgccatt
     9301 ttaccgatgc aacactgctt tcggcgatga ccgggatcgc gcgctttgtg caggacaaag
     9361 atctgaaaaa gatccttcgt gcgaccgatg gtctggggac agaagcaacc cgtgccggga
     9421 ttattgaact gctgttcaag cgtggttttc tgaccaaaaa agggcgctat atccactcta
     9481 ccgacgccgg aaaagcgtta ttccattcgc tgccagaaat ggcgacgcga ccggacatga
     9541 ccgcgcactg ggaatcggtg ctgacgcaaa tcagcgaaaa gcagtgtcgc tatcaggact
     9601 ttatgcagcc gctggtgggg acgctatatc agcttattga tcaagccaaa cgtacgccgg
     9661 tgcggcagtt tcgcggcatt gtggctccgg gcagtggtgg cagtgctgat aagaaaaagg
     9721 ctgcaccgcg taaacgtagt gcgaaaaaaa gtccgccagc agatgaagtc ggaagcgggg
     9781 cgatagcgta agcgagtgaa tctttcgtgc tattcgagtc atattctgaa atatccagcg
     9841 aatcaagaaa attcgttgga tatttttttg catggataaa attatcgcct ctaaagtatg
     9901 taataacagg gaatgtgatg aaaaaagttt tattacaaaa ccatcctggg agcgagaagt
     9961 attcttttaa tggctgggaa atatttaata gtaattttga acggatgatt aaagaaaata
    10021 aggccatgct gctttgtaag tgggggtttt atttaacatg tgttgtcgct gtaatgtttg
    10081 tgttcgcagc gataacatcc aacggtttga atgaaagagg cctgattacc gcgggatgct
    10141 cttttcttta tctattaatt atgatggggc ttattgttcg ggccggtttt aaagcaaaaa
    10201 aagaacaact gcattattat caggctaaag gtattgagcc gctcagtatc gagaagttac
    10261 aggcgctaca attgatcaca ccttatcgat tctatcataa gcaatggtct gaaacgctgg
    10321 agttctggcc gcgaaagcct gaacctggca aagatacctt ccaatatcat
//
LOCUS       DAJTMC010000146        10334 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.146, whole genome shotgun sequence.
ACCESSION   DAJTMC010000146 DAJTMC010000000
VERSION     DAJTMC010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10334
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.146"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            121..987
                     /gene="folD"
                     /locus_tag="OO130_003288"
     CDS             121..987
                     /gene="folD"
                     /locus_tag="OO130_003288"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HCR5366575.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQACIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            989..1201
                     /gene="ybcJ"
                     /locus_tag="OO130_003289"
     CDS             989..1201
                     /gene="ybcJ"
                     /locus_tag="OO130_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HCR5366576.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            1309..1830
                     /locus_tag="OO130_003290"
     CDS             1309..1830
                     /locus_tag="OO130_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR5366577.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKVIPPRLLFAGIILAMLPDADVLS
                     FKFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTPYGHQVIISELMWVWLP
                     GMVLMEMLWWRRR"
     gene            complement(1866..3251)
                     /gene="cysS"
                     /locus_tag="OO130_003291"
     CDS             complement(1866..3251)
                     /gene="cysS"
                     /locus_tag="OO130_003291"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HCR5366578.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQTRAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            3425..3919
                     /gene="ppiB"
                     /locus_tag="OO130_003292"
     CDS             3425..3919
                     /gene="ppiB"
                     /locus_tag="OO130_003292"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HCR5366579.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            3922..4644
                     /gene="lpxH"
                     /locus_tag="OO130_003293"
     CDS             3922..4644
                     /gene="lpxH"
                     /locus_tag="OO130_003293"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HCR5366580.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            4762..5271
                     /gene="purE"
                     /locus_tag="OO130_003294"
     CDS             4762..5271
                     /gene="purE"
                     /locus_tag="OO130_003294"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HCR5366581.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGAA"
     gene            5268..6335
                     /gene="purK"
                     /locus_tag="OO130_003295"
     CDS             5268..6335
                     /gene="purK"
                     /locus_tag="OO130_003295"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HCR5366582.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETVLTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWRLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YMGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDANYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(6472..7363)
                     /locus_tag="OO130_003296"
                     /pseudo
     CDS             complement(6472..7363)
                     /locus_tag="OO130_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
     gene            complement(7360..8175)
                     /locus_tag="OO130_003297"
     CDS             complement(7360..8175)
                     /locus_tag="OO130_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HCR5366583.1"
                     /translation="MTIIHPLLASSSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     SHPQPLAAAWMQRAEETGLFGPLAMAASDPLTAELRQFRHCFQAALNGVKTDWRHWLG
                     KGPGLTPSHDDTLSGMLLAAWYYGALDARAGRPFFACSDNLQLVTTAVSVSYLRYAAQ
                     GYFASPLLHFVHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            complement(8186..9445)
                     /locus_tag="OO130_003298"
     CDS             complement(8186..9445)
                     /locus_tag="OO130_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HCR5366584.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPAFSLISELNEGKTLLH
                     AGPPMRWQEMTGPMKGACVGGCLFEGWAKDEAQALAMLEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRTLAPQIARLDHDKQHIAEV
                     IDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIYLERNMQLQIPGWDFQGACLGLDIRRVVETGITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
     gene            complement(9455..10334)
                     /gene="fdrA"
                     /locus_tag="OO130_003299"
                     /pseudo
     CDS             complement(9455..10334)
                     /gene="fdrA"
                     /locus_tag="OO130_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415051.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
BASE COUNT         2275 a         2935 c         2774 g         2350 t
ORIGIN      
        1 aaccttcgca agaggtcgct tcacgcgata aatctgaaac gaaacctgac agcgcgcccc
       61 gcttctgaca aaataggcgc atccccttcg acctacgtaa cagatggaat cctctctctg
      121 atggcagcaa agattattga cggtaaaacg attgcgcagc aggtgcgctc tgaagttgct
      181 caaaaagttc aggcgtgtat tgcagccgga ctgcgggcac caggactggc cgttgtgctg
      241 gtgggtagta accctgcatc gcaaatttat gtcgcaagca aacgcaaggc ttgtgaagaa
      301 gtcgggttcg tctcccgctc ttatgacctc ccggaaacca ccagcgaagc ggagctgttg
      361 gagcttatcg atgcgctgaa tgccgacaac accatcgatg gcattctggt tcaactgccg
      421 ttaccggcgg gtattgataa cgtcaaagtg ctggaacgta ttcatccgga caaagacgtg
      481 gacggtttcc atccttataa cgtcggtcgt ctgtgccagc gcgcgccgcg tctgcgtccc
      541 tgcaccccgc gcggtatcgt cacgctgctt gagcgttaca acattgatac cttcggcctt
      601 aacgccgtgg tgattggcgc atcgaatatc gtcggtcgtc caatgagcat ggaactgttg
      661 ctcgcaggtt gcaccactac cgtgactcac cgtttcacta aaaatctgcg tcaccacgta
      721 gaaaatgccg atctgttgat cgttgccgtc ggcaagccgg gctttattcc tggtgactgg
      781 atcaaagaag gcgcaattgt gattgatgtc ggcatcaacc gtctggaaaa tggcaaagtt
      841 gtgggcgacg tcgtgtttga agacgcggct aaacgcgcct catacattac gcctgttccg
      901 ggcggcgttg gcccgatgac ggttgccacg ctgattgaaa acacgcttca ggcgtgcgtt
      961 gaatatcatg atccacaggg tgagtaacat ggcgacattt tctttaggta aacatccgca
     1021 cgttgagctg tgcgatttgc tgaaactgga aggctggagc gaaagcggcg cgcaggcgaa
     1081 aatcgcgatt gccgaaggcc aggtgaaagt cgacggtgcg gttgaaacgc gcaaacgctg
     1141 caaaatcgtc gccggtcaga cagtgagttt tgcaggtcac agcgtacagg ttgttgcctg
     1201 atccctttcc cggcctgtat caacaggccg gttctctttt cagacttttg ccctctacgc
     1261 cccgttcatc catgctgata gcatcgacat tttgccgaag cgaccatcat gccaaccgtt
     1321 attacgcacg ccgccgttcc cctatgcatt ggtttagggt tgggttcgaa agtcatccct
     1381 ccgcgtctgt tatttgccgg aatcatcctg gcgatgctgc ccgacgccga cgtattgtcg
     1441 tttaaatttg gcgttgctta cggcaatgtt tttggtcatc gcgggtttac ccattcgctg
     1501 gtgtttgcgt ttgttgtccc gctgttatgt gtgctgattg gacgccgatg gttcagggct
     1561 gggcttattc gctgctggct gtttttaacc gtctcgttgc tatcgcacag cttgctggat
     1621 tcggtaacca ctggcggtaa gggcgttggc tggctgtggc cgtggtcaga tgaacgcttt
     1681 ttcgctccct ggcaggtgat taaagtcgcg ccgtttgcat tgtctcgtta caccacgccg
     1741 tacgggcatc aggtgataat ttctgaattg atgtgggtgt ggttgccggg gatggtgctt
     1801 atggaaatgt tgtggtggcg caggcgataa ccggatgcga aaactcgcat ccggcaacag
     1861 cgcaattact tacgacgcca ggtggtccct tgcgggccat cttccagcac gatccccatc
     1921 tcgttaagac ggtcacgcgc cgcatctgcc gccgcccagt ctttcgcttt acgggcatcc
     1981 agacgctgtt gaattaacgc ttcaatctca gccacttcgc tgtcgtctgc ctgcgcgccg
     2041 ctttgcagga acgcttccgg ttcttgctcc agcaggccca atacagcgga aagtttacgc
     2101 aggtgagatg ccattgcatt cgccgctgcc atatcttctg ctttcagacg gtttacttca
     2161 cgcgccatat caaacagtac ggaataggct tccggggtgt tgaaatcgtc gtccatcgct
     2221 tcaataaagc gcgcttcaaa cgcttcgcca ccggcaggcg caacggtttt atctgtgccg
     2281 cgcagcgcag tgtagagacg ctccagcgcc gcacgcgtct gcttcaggtt ctcttcgctg
     2341 tagttcaact ggctgcgata gtggcccgac atcaggaagt aacgcacggt ttccgcgtcg
     2401 tagtatttca gcacatcgcg cacggtaaag aagttaccca gcgatttgga catcttctcg
     2461 cggtcaacca tcaccattcc ggagtgcatc cagtagttca catactgacc atcatgggca
     2521 caggtggact gcgcgatttc gttctcgtgg tgcgggaaca tcaggtctga accgccgccg
     2581 tggatatcaa agtggttacc cagctgcttg cagttcatgg ccgaacattc aatgtgccag
     2641 ccaggacgac ccgcgcccca cggagacggc cagctcggtt cgccctcttt cgacatcttc
     2701 cacagaacga agtccatcgg gttgcgtttg tcatcaacca cgtcaacgcg cgcgccagcc
     2761 tgcagctggt cgagatcctg acgcgacagc acgccataag tcggatcggt cgggacgtcg
     2821 aacatcacgt cgccgttgtc agccacataa gcgtgacctt tggcgatcag ttgttcagtg
     2881 agttcaataa tttctgcgat atggtgcgtc gcgcgcggct ccatatccgg gcgcagaatg
     2941 ttcaaagcat caaaatcttt gtgcatttcg gcgatcatgc gatccaccag cgccacaaag
     3001 ctttcgccat tttcattggc gcgtttgatg attttgtcgt cgatatcggt aatgttgcgc
     3061 acatacttca gcttatagcc gaggaaacgc agatagcgcg caaccacgtc aaaagcaaca
     3121 aaggtacgcc cgtgaccgat atgacagaga tcgtaaacgg tgattccaca cacgtacatg
     3181 ccgacttccc cggcgtgaat aggcttaaat tcctcttttt ggcgtgtcag agtattgaag
     3241 atttttagca tcgaagattc cgtttagaca tgtgtgggta attgagttgc gtataatacc
     3301 catatttccc gcgcgaatca gcatacattg cgtgatgatc cgatcgtgcg gttatgctat
     3361 aacaccaccc tatatatgac ccgaaccggg ttgaagcacc aatcaaacgg aacaggatgc
     3421 aaaaatggtt actttccaca ccaatcacgg cgatattgtc atcaaaactt ttgatgataa
     3481 agcacctgaa acagttaaaa acttcctgga ctactgccgc gaaggttttt acaacaacac
     3541 cattttccac cgtgttatca acggctttat gattcagggc ggcggttttg aaccgggcat
     3601 gaaacaaaaa gccaccaaag aaccgatcaa aaacgaagcc aacaacggtc tgaaaaatac
     3661 ccgtggtacg ctggcaatgg cccgtactca ggctccgcac tctgcaactg cacagttctt
     3721 catcaacgtg gttgataacg acttcctgaa cttctctggc gaaagcctgc aaggttgggg
     3781 ctactgcgtg tttgctgaag tggttgaagg catggacgtg gtagacaaaa tcaaaggtgt
     3841 tgcaaccggt cgtagcggta tgcaccagga cgtgccaaaa gaagacgtta tcattgaaag
     3901 cgtgaccgtt agcgagtaat cgtggcgaca ctctttattg cagatcttca tctctgcgtg
     3961 gaagaaccgg cgatcaccgc cggttttctg cgttttttag cgggggaagc tcgcaaggcc
     4021 gacgcgctgt atattcttgg cgatctgttt gaagcgtgga ttggcgacga cgatcccaat
     4081 ccactccatc gccagatggc ggcagcgatc aaagcggtgt ccgattccgg cgttccctgt
     4141 tatttcattc atggcaaccg tgattttctg ctcggcaaac gctttgcccg tgaaagtggc
     4201 atgacgttat tgccggaaga aaaggtgctc gaactttatg gccgccgggt gttgattatg
     4261 catggcgaca cgctgtgcac cgatgacgcg ggttatcagg cttttcgcgc caaagtccac
     4321 aaaccctggc tgcaaatgct attcctcgcc ctgccgttgt ttgtgcgcaa acgcattgcc
     4381 gcgcgaatgc gcgcgaacag caaagaagcc aacagcagta aatcgctggc gatcatggac
     4441 gttaaccaaa acgcagtggt cagtgcgatg gaaaaacatc aggtgcaatg gctgatccac
     4501 gggcataccc atcgcccggc ggtgcatgaa cttatcgcca accagcaacc tgcttttcgc
     4561 gtggtactgg gtgcctggca tacggaaggt tcgatggtga aagtcacggc ggatgacgtt
     4621 gagctgattc attttccgtt ttaaaaaaac cacaactttg ctgattttac agccacgcaa
     4681 ccgttttcct tgctctcttt ccgtgctatt ctctgtgccc tctaaagccg agagttgtgc
     4741 accacaggag ttttaagacg catgtcttcc cgcaataatc cggcgcgtgt cgccatcgtg
     4801 atggggtcca aaagcgactg ggctaccatg cagttcgccg ccgaaatctt cgaaatcctg
     4861 aatgtcccgc accacgttga agtggtttct gctcaccgca cccccgataa actgttcagc
     4921 ttcgccgaaa gcgccgaaga gaacggttat caggtgatta ttgcgggcgc aggtggcgca
     4981 gcgcacctgc caggcatgat tgccgccaaa acgctggtgc cggtgctggg cgtgccagta
     5041 cagagcgccg cactgagcgg tgtcgatagc ctctactcca tcgtacaaat gccgcgcggc
     5101 attccggtgg gtacgctggc gattggtaaa gctggcgcgg caaacgcggc attactggca
     5161 gcacaaattc tcgcgactca cgataaagag ctacaccagc gtctgaatga ctggcgcaaa
     5221 gcccagaccg acgaagtgct ggaaaatccg gacccgcgag gtgcggcatg aaacaggttt
     5281 gcgtcctcgg taacgggcag ttaggccgta tgctgcgtca ggcaggcgaa ccgttaggca
     5341 ttgctgtctg gccggtcggg ctggacgctg aaccggcggc ggtgcctttt caacaaagcg
     5401 tgattaccgc tgagatcgaa cgctggccgg aaaccgtatt aacccgcgag ctggcgcgtc
     5461 atccggcctt tgtgaaccgc gatgtgttcc cgattattgc tgaccgtctg actcagaagc
     5521 agcttttcga taagctccac ctgccgaccg cgccgtggca gttacttgcc gatcgcagcg
     5581 agtggcctgc ggtgtttgat cgtttaggtg aactggcgat tgttaagcgt cgcactggtg
     5641 gctatgacgg tcgcggtcaa tggcgtttac gcgccaatga aaccgaacag ttaccggcag
     5701 agtgttacgg cgaatgtatt gtcgagcagg gcattaactt ctctggtgaa gtgtcgctgg
     5761 ttggcgcgcg cggctttgat ggcagcaccg tgttttatcc gctgacgcat aacctgcatc
     5821 aggacggtat tttgcgcacc agcgtcgctt ttccgcaggc caacgcacag cagcaggcgc
     5881 aagccgaaga gatgctgtcg gcgattatgc aggagctggg ctatatgggc gtgatggcga
     5941 tggagtgttt tgtcaccccg caaggtctgc tgatcaacga actggctccg cgtgtgcata
     6001 acagcggtca ctggacacaa aacggtgcca gcatcagcca gtttgagctg catctgcggg
     6061 cgattaccga tctgccgtta ccgcaaccag tagtgaataa tccgtcggtg atgatcaatc
     6121 tgattggtag cgatgcgaat tatgactggc tgaaattgcc gctggtgcat ctgcactggt
     6181 acgacaaaga agtccgtccg gggcgtaaag tggggcatct gaatttgacc gacagcgaca
     6241 catcgcgtct gactgcgacg ctggaagcct taatcccgct gctgccgcca gagtatgcca
     6301 gcggcgtgat ttgggcgcag agtaagttca gttaagcagg tgctcatttg attgccggat
     6361 gcggcgtaaa cgccttatcc ggcttaccgc tccggtaccc attgtaggcc tgataagacg
     6421 cgtcaagcgt cgaatcaggc atggtgcacc gattgccgga tgctctcatc actacagcga
     6481 aatacaggtc cccgcttcgc ccgccagcgt ctcttcaatt cgcgataacg ccccaatcca
     6541 cgcgggttta ctacggcttc tgacataacc actcaccgcc gttaccttcg gccccatcga
     6601 accatcggct ttggcaaatg gcgctaactc atccggtgtg gcatggcgaa tggcacgttg
     6661 ctgcggcgtt ccccagtttt catataccgc atcagcatcg gtgaggatca ccagtccatc
     6721 ggcattaatc tgctcggcga gcaacgcagt ggcgagatct ttatcaatca ccgcttcact
     6781 ccctgcccca tcttccgtca caggcacacc gccgccgcca ctgcaaatca ccacatgccc
     6841 ctctttgagc aacaactcga tggcttcgct gtcgagaatt ttacgcggtt gcggagacgc
     6901 caccacccgg cgcaaatatt taccatcacg tttcatctgc cagccgtaag ccgcttccag
     6961 tgcctcttgt tcttctggct gataaaccgg accaataaat ttctctggct gcaaaaacgc
     7021 cggatcatca ggcgaaacct caatgcgcgt cagcaccgtc gtaacaggcg gtatctgcgg
     7081 ctgtgcgctc aagctctgcg ccagcatata gcctcatccc ctggctttcc gccaccagca
     7141 catccagcgg atacggttct acctctttcc acgccagatt ctgtaacgcc agcagcccca
     7201 cctgcggccc gttgccgtga acaatcgcca gccgataaga acgggccagg cgtgccagcg
     7261 cgggtacagc actggcgata ttgcgatatt gattttccgc cgtcagcgcc tcaccgcgct
     7321 ggagtaaggc gttgccccca agagctacaa ccagtgtttt catggttttc cttgtaataa
     7381 ttgttggcca agccagaacc ccagcagcgt atctgcccct gacgtatgcc ccagcgccag
     7441 cagcgaatca atcgcaacag cggtacgttt cgggcaactc agagcatgga caaagtgcag
     7501 gagtggcgag gcgaaatatc cttgcgcggc ataacgtaaa taactgacgc tcaccgctgt
     7561 ggtaacgagt tgaagattgt cggaacaggc aaaaaacggg cgaccggcgc gcgcatctaa
     7621 agcgccataa taccaagccg ccagcagcat tccgctcaac gtgtcatcat gacttggcgt
     7681 taatccgggg cctttaccca gccagtgccg ccagtcggtc ttaacgccat tgagcgcggc
     7741 ctgaaaacag tgacgaaact ggcgtaattc agcggtcagc ggatcgcttg ccgccatcgc
     7801 cagtggtccg aaaagtccgg tttcctccgc gcgttgcatc catgcagctg caagcggttg
     7861 aggatgcgaa ggcggcgtaa tacgcagcaa acaataacgc tgtggctgtt taaccgtgaa
     7921 acgcccgagg cgaatccctt gagccacaac ctgtggtcgt tcattgccgc ataatccacc
     7981 gcataacgca tcgaattgcg cgcggcgaag cacccatcct ccggggccga aaccactacc
     8041 ctgacgatgc agcgttaaca gttcgccgct ttccgtcatc aggttaatcg cccgccgcca
     8101 cactcccgct aaccgccagg actggcgata attcggtgcg ctactgctgg caagcagagg
     8161 atggatgatc gtcattgcgc gttcctcaac caatccccat gctttcagcc agtgcttcca
     8221 gcgcctgttc aaagcacgcc agcggtgccc gcacggtgcc tgcgccaatc tgcccgatcc
     8281 ccgcctcttt atgggcgata ccggtattga tgagtggcgt aatgccggtt tctaccacgc
     8341 gacgaatatc cagccccagg cacgcgccct gaaaatccca gcctgggatc tgcaactgca
     8401 tattgcgttc aaggtaaatt tccgccatct cttcagatac cgccctggcc gcttccatgc
     8461 cacccgcacc gacaaagcgc gttacgccag gcgctgcgat cattgccgca cctccgatac
     8521 caaaggtttc ggtaatcgcg ctatcgccca tatccgggtt cgcctgctcc tgcgagaagc
     8581 cggtgaaaaa cagaccttgc ggagtgttta caggcgcagt aaaccagcgt tcacccagcc
     8641 cgcttacccg aatcccgaac atattgccgt tgcgggtcat agccgtaacg atgctgcctg
     8701 cgcggatcat cgcgccagca tccatcgccg ccttgcagta ggccatcgcg aggttgagga
     8761 agaactgatc ggtcacgctg aggaaatcga tcacttccgc aatgtgctgt ttatcatgat
     8821 cgaggcgagc aatttgtggg gccagcgtac gcattaacag tgctgaggaa gcaatattgc
     8881 gttgatggaa ctcatcgccc atcgtaatgc cctgcgccat catcgccgtg agatcgatac
     8941 cgcgctccat gcgccccagc gccgcgctta atactggcat taacacatcg cgcatccagc
     9001 gatggcgagt caggacatct tcgccgtaag cgccaaaacg catcactttg ccgatacctt
     9061 cgttgaggtt gcagtacgcc cggttgccgt cggtcacgtt ctcgaccacc agcatcggca
     9121 tactggcaga agtaatgccg cccattggcc cgacggcatt cacatggtga caaggaatga
     9181 agttcacttc cccctgctcc agcatcgcca gtgcctgcgc ttcatctttc gcccagcctt
     9241 cgaacagaca tccgcccacg cacgcccctt tcatgggtcc ggtcatctcc tgccagcgca
     9301 ttggcggccc ggcgtgaagc agtgttttgc cctcgtttag ttcgctgata agtgaaaaag
     9361 ccggttgcac atccagccag tgtggacgag cacgacgaat ttgttcgata accgcagcat
     9421 tggcttgcgc cactgatgta aacatgggaa ccccttattg caaacgttct aataaacgag
     9481 ccagtttttt attgccgccg gcgactggcg accattggta atgcacaacc ggtttgctgg
     9541 cgctttgtag ctccagcgca aagctgcgta atccgatgtt aatcacggcg acgttttcca
     9601 gtaatgacgg tgtatgttgc tgtgtggcag gcgagagcgg ataaattaac gctgccgcca
     9661 gcaaggtggc ttccggcagc gaactcacta ccgcgatccc cgcatcttcc agcgtggcga
     9721 tttgctgcga gcggcattgc gggtcacgtt cagtgcctgt caccgtggca atggcataca
     9781 gtggttgatt atctgaacgc gcggcacagg ctttttgcta ggcgcttacc agcgaggcgg
     9841 caggatcggc agtcgcaccg aagccaatca cgacatcaag tagcaatacg cgcacttgtg
     9901 gtttagcgcc gagatcggca attaactggt tgcgtaaggc tgggtcgatc atcggatggg
     9961 gacgcccgac ggtgtagaaa tcatcgccga ggtcaataat ctggtgacca tcggcgtcga
    10021 gcatcatgcc gtgatgatgg gtatcgtcgg cttccacgcc aaggtgtcca gcaagtaatc
    10081 ccgccgcttc ggcagccagc gtaccgccgg tatacaaacc gcaaataaat ccgctgctga
    10141 caggcgctat tgcgttacat cgcgccgtga cgcgtgaaag caggcaagcc aggcgtgcgg
    10201 cctcatccag cgaggaggca aaccagacat tctcgtcgcg ggccaccgcc ggggtataac
    10261 ctaaaaacag cgccaccatc ggtttgccgg ttgctttcat ggcattaaca attttcagac
    10321 gcacagcttc ggca
//
LOCUS       DAJTMC010000147        10326 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.147, whole genome shotgun sequence.
ACCESSION   DAJTMC010000147 DAJTMC010000000
VERSION     DAJTMC010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10326
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.147"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(1..869)
                     /locus_tag="OO130_003300"
                     /pseudo
     CDS             complement(1..869)
                     /locus_tag="OO130_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310631.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
     gene            complement(844..1644)
                     /gene="otsB"
                     /locus_tag="OO130_003301"
                     /pseudo
     CDS             complement(844..1644)
                     /gene="otsB"
                     /locus_tag="OO130_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416411.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
     gene            complement(1811..2797)
                     /gene="araH"
                     /locus_tag="OO130_003302"
     CDS             complement(1811..2797)
                     /gene="araH"
                     /locus_tag="OO130_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HCR5366585.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGILCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            complement(2812..4326)
                     /gene="araG"
                     /locus_tag="OO130_003303"
     CDS             complement(2812..4326)
                     /gene="araG"
                     /locus_tag="OO130_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP binding protein
                     AraG"
                     /protein_id="HCR5366586.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADQHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGAIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(4396..5384)
                     /gene="araF"
                     /locus_tag="OO130_003304"
                     /pseudo
     CDS             complement(4396..5384)
                     /gene="araF"
                     /locus_tag="OO130_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
     gene            6098..6601
                     /locus_tag="OO130_003305"
     CDS             6098..6601
                     /locus_tag="OO130_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HCR5366587.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(6680..6931)
                     /locus_tag="OO130_003306"
     CDS             complement(6680..6931)
                     /locus_tag="OO130_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HCR5366588.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFSNHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(7046..7132)
                     /gene="azuC"
                     /locus_tag="OO130_003307"
     CDS             complement(7046..7132)
                     /gene="azuC"
                     /locus_tag="OO130_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HCR5366589.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            7395..7718
                     /locus_tag="OO130_003308"
     CDS             7395..7718
                     /locus_tag="OO130_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HCR5366590.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            7889..8386
                     /gene="ftnA"
                     /locus_tag="OO130_003309"
     CDS             7889..8386
                     /gene="ftnA"
                     /locus_tag="OO130_003309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HCR5366591.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQHLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(8424..8663)
                     /locus_tag="OO130_003310"
     CDS             complement(8424..8663)
                     /locus_tag="OO130_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HCR5366592.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSKDGFSTDQSKICRH"
     gene            8854..10065
                     /gene="tyrP"
                     /locus_tag="OO130_003311"
     CDS             8854..10065
                     /gene="tyrP"
                     /locus_tag="OO130_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HCR5366593.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNVRKLRRVF
                     ITGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVR"
     gene            complement(10127..>10326)
                     /gene="yecA"
                     /locus_tag="OO130_003312"
     CDS             complement(10127..>10326)
                     /gene="yecA"
                     /locus_tag="OO130_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HCR5366594.1"
                     /translation="EKMSPEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEK
                     PGRNDPCPCGSGKKFKQCCLH"
BASE COUNT         2599 a         2586 c         2395 g         2746 t
ORIGIN      
        1 ataacgaatt ttaccatgat gctgcggata tttttccagc aacgcttcat aggcgagaaa
       61 acgctctggc aaacctttgg aataatccag ccgttcgaca gaaaagatat tttgtacgtt
      121 tttcagttcc gctttaagtt gcgccagttt tggcggcagt ggcccggcag cctgtttggc
      181 tatttctttc ggttcaatgc cgatcgggta gacttctgtt cgaaatgctt tgccccaggc
      241 tgtatggctt tttgcgctac gtgtcgtgac gcgggtcagg ttagaaagac aatccaggaa
      301 cgccagacga tcgttttctg tctggaaacc cagcaaatca taatcacaaa gctgttcaag
      361 caaggtgtca tatgtcggca gcgcgttgaa gatttccggt gtcgggaaag gaatatgcag
      421 aaagaaacca atgcgattat tcaccccacg tttgcgtaat tcatgcgcaa agggcaacag
      481 gtgataatcg tggatccaga taatgtcatc gtcttgcaac agcggcagta atttatctgc
      541 cagcaacgca tttacgcgta gatagccgtc ccaggcagga cgctgaaatt gcatcagatc
      601 gagccgataa tgaaaagcgg gccagagaac ggcattggag aattggttgt agtattcgtc
      661 aaggtcctgt tcgctgaggt taaaagaggc ccacgtaatg ttaccttttt tagcggctga
      721 tcctcattcc ctgtttcacc actccagcca aaccacagtc cgcctgtggc tttcagtgcc
      781 cccagtatgc caacggcaag gccaccggca ctggcggcgt gctcgtctgg tggtgcaatc
      841 cggttagata ctacgactaa acgactcata gtcatcactc ctgttatttt ctcttttttg
      901 ttgtaatgcg gtggttatca tttcaagcct gctccagaca tccggcacac ccgccagtcg
      961 ccatgatgcc tgagttgcac ctgtgccaat ttttactgac attccgccca gtcggttaac
     1021 gactgcgaag ccagattcat cggttaaatc atcgcccaga aatacgggcg ttcgcccgat
     1081 aaagggagct tcctgcataa aagctgcaat tgcctcacct ttactggtac ctctcggttt
     1141 gatctcgaca acacactttc cctgctgtaa cgccatttgt ggccagatct gagtaatacg
     1201 ttacgctaat gtcattaatg cgtcttcatg ctgcggagcc tgacgataat gcagcgcaaa
     1261 agccatccct ttcgcctcca gctccgcgcc gggatactga gcgataactg tatgcagttg
     1321 cacgctaata tcacgcgcaa tcgcatccgg cagatgaacg atatgcgttt taccattgat
     1381 gtcacggcgc tccgccccat gcacgcccgc taacgggaag cgataaggtt ttgccagtgc
     1441 gtcaagctcc accattgagc gccctgatat caatgccaat gcaccatcac ttgcggttgc
     1501 cagtagctgt agtccttgca gaatattgtc aggcacgacg acctgatcgg gatgcggttt
     1561 gatttccgcc agcgttccat cgagatcaaa aaaccaggca tatttcgcgg atagttcagg
     1621 ggtttcggtt aacggttctg tcacccggtt ctcctacttc tcttttctta ttgttttgcg
     1681 cagaacgcgc tttacagaca tgtaagtata gacagtgtga cgggggtcgc catttgcaac
     1741 gaagaacagc caaccgttat ggcggttggc tgtggtggga aaaaacgcta aattgttgca
     1801 gaaaaaagca tcagacagtg cgtttcgctt tttgcttgta acggtcgaag atcaccgctg
     1861 ccagcaggat taagccgcga accacgtact gcgcgaaagg agaaatatta agcaggttca
     1921 tggcgttttc cacggtgcct aaaattaaga tacccgccac cacatatgag atttttccga
     1981 tgcctccttt cagagaaacg ccacctaaaa cacaggcgga gataacaatc agctcgtaac
     2041 caatcgacgt cattggctgc ccactggtca tacgtgaagc cagaataatt ccggctatcg
     2101 cagataccag gccagagaga acaaagataa taattttggt gcgaacaacc ggtacacccg
     2161 ccagacgcgc agcctcttcg ttcccgccaa tcgccagggt gttacgacca aaggtggttt
     2221 tattcagcaa caaaccaaag ataatcagac acgcgacggt gagccagatt ggcgcaggca
     2281 gaccgaacca gttagcgtaa ccaagggcaa agaagctttc atcttcgata ccgaccgctt
     2341 taccgtctga aatgatgtac gccagacctc gaacaatctg catcgttgcc agtgttgtga
     2401 tcagagcatt tattttcagt ttggcgataa caaagccatt gaccaggcca cagagaatgc
     2461 caagcagcaa ccccgctgcc acgccaatcc acaggctttc agtcaggttg ataaccaccg
     2521 ccgtggtgac acccgcacag gcaattacgg aggcgacaga aaggtcaaag tcaccggaag
     2581 caaggcagaa caacatgcca cacgccacca tccccgacat ggaaattgcc aggcccaacc
     2641 ctttcatatt aatgaaggtg gcaaaatttg ggacaaaaat ggcacaggcg ataaagagca
     2701 ccgcaaacac caccagcatg ccgtactgat cccagatacg cccgaagctg aatgacgact
     2761 taggtgcgcc agaacccgat gtagaaacag aagacatcat actctcctta ctcaggcaac
     2821 tgcctggctg actttaggca tcgcaaggct cagtgcctga cgctcatctg cctgctcgtg
     2881 taacaattca ccggcgattg caccttcccg catcaccaca atccggtcgg caacgccgag
     2941 gacttcaggt aagtcgctgg aggcaaacag caccgccaca ccctgcgccg ccagcgcata
     3001 aattacgtta tatatttcgt gcttagcacc aacatcaatg ccgcgcgtag gttcatccag
     3061 caaaatgacc ttcatctctt ccgataacca gcggcccaga atggcttttt gctgatttcc
     3121 gcctgagaga ttcatgatca gttgctcagc gcccggcgtt ttgatgttga gcgaacgaat
     3181 gtgctgatcg gcattgtttt cttcccaacc gttgttgatt acacaaccgc cgagcacatg
     3241 tttgcgtctg gcactgatgt tgatattgtc gcgaacggag tgcacgggaa taatgccttc
     3301 cgctttgcga tcttccgggc agagcatcat gcctgcggca atggcgtggc tcggtttacg
     3361 aatatcgatc ggctgttggt cgatataaac ctgaccggcg gtgatttgcg tcccgccaaa
     3421 caagcctttc attaattcgc tacgccccgc ccctaccaga ccaaacagcc caacaatttc
     3481 accactgcga accgccagac ttattggcgt acgcacgcct ggtgctttca cagcatcaag
     3541 acgcaggcgc tcctcgccat aactacgagg ttgccagccg tagatatcgc caatgtcgcg
     3601 cccgaccatc gcctgcacca gcgcgtcgtg gtcaacctgc tgcatatcgg taaaggtttt
     3661 gacataacgt ccatctttaa agacagtgat ggcatcgctg agggcaaata tttcttccat
     3721 acggtgagaa acgtataaga tcacccgccc ctcttttcgc agttcacgaa taacgcggaa
     3781 aagattgtcg atttcgcggg cagaaaggga gctggttggc tcatcaaagg cgataatttt
     3841 agcgttacgc gccagcgcct tggcgatttc aaccatctgc cactgaccaa tggagagata
     3901 tttcagcggc gtgtccgggt caatatccat accaagatgt ttaagttgta aacccgcctc
     3961 ataattcagc aatgagcgat tcacaatgcc gcctttatgc ggcagctggc cgagatagat
     4021 gttttccgcg acggtcattt ccggcacgag atgcagttcc tggtaaataa tcgccacacc
     4081 cgcgttaagt gctgcggtcg tgtcggaaaa ggacatttcc tgcccattaa tcactacaga
     4141 acccgtggtt ggcgcatagt tgccgctgag gatttttaag agagttgatt ttcctgcgcc
     4201 attttcaccc atcaacgcat gaacctgacc ggcatagcag tcaaaactaa tatccgtcag
     4261 cgccttaacg ccgggaaacg ttttaccgat gccgcgaaat gagagatacg gggtagactg
     4321 ttgcataacg tctccgtgaa tcactggtcg ttcacacccc ctctgcatca tgcagagggg
     4381 aatttttccg gcaaattact taccgcctaa accttttttc tccagttctt ctttaaagtt
     4441 gtcacgcgtg atcagcacca cgtcggtaac ttcggtaaat tttggcggtt caacgtcttt
     4501 tgctacccag ttgtaaagca tttcgctgga tttatagcca tgtacgtccg ggcttggcag
     4561 cagggaaccg tagaagccgg ttgcctgtac tttagacagt tcgctcaccg catccacacc
     4621 gttaatgcca atgccgataa tatcggcagc tttaaagccc tgaccttccg tcgcgcgtac
     4681 gccgcccagc acggtgctgt cgttcatacc gacgatcagc caatgtttaa cttcaggatg
     4741 ttgaaccagc attgagttgg cagcgtcaaa tgcccccggg atgtcgttag atttggtagg
     4801 tacctgataa atttgttttt ccgggaatcc ggccgctttc agcgcatcca tagatcccgt
     4861 agtacggcgg cgggcagtat ccagttcgtt ggcggtaatc accatcaccg cgctttcttt
     4921 gacatcccag ccacgtttct gcatctcttt atacagttcc tgaccctgac gttcgccaat
     4981 tttagtcgcg ccatcatcac cagcggaacg gtatccattg gcttaccttt ggcgttaaca
     5041 aactgatcat ccacggcaat gactttcata tcgtagccac gcgctttcgc gacgatggca
     5101 gagccgaatt tggggtccgg agtacaaata acgaaacctt ttgcgccact ggcagccagg
     5161 ctgtcgatcg cgttcaatgt tttttcgcca tccggcacgg caatcttaat aacctcaaac
     5221 cctaaatcct tcccggcttt atcggcaaac ttccattcgg tctggaacca cggctcttcc
     5281 ggttgcttca ccagaaaacc gagcttcagg ttctccgcca tagcggattg tgacataacg
     5341 gctgccagac caatggctgc cagggcttta gtaaatttgt gcatggttct ctccagcttt
     5401 agtgtcgttt tgtgtagggc aaaaacgaat gacattcgtt aaattaatcg gaaaacaaag
     5461 cattaccttt taactaaaag ataagtgacc gtgttgacat agttttagcg agaaattaat
     5521 tctccatagg agagcaatat cacatcgcag aattacagtg agaacgtgca taaatttagc
     5581 gggaaaagac ataagggaaa gccaatttgt cagacaaatt gtcgaatgca cagcagatta
     5641 atccataaga ttagcttgga aatccttgtt gtctttggta cccatgcggg atgtcttctt
     5701 tttaaccagt caataggccg cattacctgg cgttgagttt ttgaaatggt gtaataaccg
     5761 caactcaaag atgtggaaaa tgcacgtcat tcatttcgcc attaattatc actgtgctca
     5821 ttaattaaca gaacacgtat aatgagagcc atctcgcaaa aatgaaaaaa cgttttataa
     5881 aatcatcatt tcatcaatga aatgtaaaaa tatataaact tgatgattta agcattttct
     5941 tatacccgtt cagacgttat tcttatttca gatcatcgtc agaattgact ccacgatcac
     6001 atttcggacc ggcagaaagg aattattctg caaacagtaa ttatggtgtt ttgatttatc
     6061 ttgcacctct ccacttctgg atataaggat attaggtatg gcaaccgctg gaatgcttct
     6121 caaactcaac tctcaaatga accgcgagtt ttacgcatcc aatctctacc ttcacctgag
     6181 taactggtgt tctgaacaga gtctgaacgg caccgccact ttccttcgcg cccaggcaca
     6241 gagtaatgtg acccaaatga tgcgcatgtt taactttatg aagagtgtcg gcgctacccc
     6301 catcgttaaa gccattgatg ttcccggtga aaaactgaac tctctggaag aactgttcca
     6361 aaaaacgatg gaagaatacg agcaacgttc tagtacgttg gcacagttag ccgatgaagc
     6421 gaaagaactg aatgatgatt caaccgtcaa tttcctgcgc gatctggaaa aagaacagca
     6481 gcatgatggt ctgttgctgc aaaccattct tgatgaagtg cgcagtgcga aacttgctgg
     6541 tatgtgccca gtgcagaccg accaacatgt tctgaatgtc gtgtcgcatc agctgcattg
     6601 atcatacatc ggcgctaatg cattgcgccg atgaagtttt tgagaaaccg ctgcctcatc
     6661 tttttgaagc agcggttttt taatgggatt cgccttgtgg ggtaaacttg agttcaatca
     6721 gcgcgatggc tttttggatt gcccgcatgg tgaccgggtc tgcggcggcg ggatggttac
     6781 taaagtcgat attcttcagc tgactggaca ttttttcacg aacttcaacg ggcgcgatta
     6841 catcgagaac atccagaatt tgtttgataa ccaactggca agcaaccaca tcagaaacca
     6901 gttcctgatc ggcattcagc ggctgggaca tcgtaaactc ctgatagcat tttgaaaacc
     6961 gttatagtag cggcttcaca tcttcagcga tagtcacatc caccgtcatc aggacacaaa
     7021 aaaacctgcc ggagcaggtt ttttgttatc ggaacatatt gcctggcggt acgtctttga
     7081 acgtcttgca atagttattg aacatacttt tcaggatttt gcgcagtttc atcgcggcac
     7141 tccgaccatt tgttatacat gtgttatttt ctttgtgctt ataatatgac aaccatcaca
     7201 aaaatcaatc tttatgtgat gcaaatcaca caaatacccc cttaatgtta taaaaatgat
     7261 aatcaaaaaa cagccccccc atttctgaca cctacagatg gcaagaaata gcgcctgcca
     7321 ggcgtctttt ccggtcattg tcgcagcgct gtaacgcgta aaatagtgct ttcttttact
     7381 cttctggctg gaccatgaga cttctgattc tgactctttc attaataacg ctggcggggt
     7441 gtacggttac tcgccaggcc catgtgagcg aagttgacgc ggcaacaggt attgtacggt
     7501 tggtttatga ccaggctttt ttgcagcatg cccataccga tcgttatgtc agtcgcggga
     7561 tcgccgatcg cgcatgccag caggaaggct atacccacgc aatccccttt ggtcagccag
     7621 taggcaactg tagccttttt gcgggttcct tatgtttgaa tacggaattc actttgtcat
     7681 atcagtgcca tcattccgcc ttccctgtct ttctctaaat attttaatca gcgaggggat
     7741 cttcgctgat taaaaaaata gcggtaatgc gttttattcc cattcgtatt tttaataatt
     7801 gaagtttata ttttaccttt tgcaaataat aaaataacaa attatagtga cgccacagca
     7861 acaaatataa cctttgtgga gcactatcat gctgaaacca gaaatgattg aaaaacttaa
     7921 tgagcagatg aacctggaac tgtactcttc actgctttat cagcaaatga gcgcctggtg
     7981 cagctatcat accttcgaag gtgctgccgc gttcctgcgc cgtcacgccc aagaagagat
     8041 gacgcatatg cagcatctgt ttgattacct gactgatacc ggcaatttac cgcgtattaa
     8101 taccgttgaa tctccgtttg ctgaatattc ctcacttgat gaattattcc aggaaaccta
     8161 taaacacgaa caattaatca cccagaaaat taacgaactg gctcatgctg caatgaccaa
     8221 tcaggactac ccaacattta atttcctgca atggtatgtt tctgagcagc atgaagaaga
     8281 gaaactgttc aaatcgatta ttgataaatt aagcctggca ggcaaaagcg gcgaaggtct
     8341 gtattttatc gacaaagaac tctctaccct cgacacacaa aactaatact ctccggcggc
     8401 aggtttactg ccgccgtttc aaatcagtga cggcaaatct tactctgatc ggtcgaaaaa
     8461 ccgtctttcg aaggtataaa tttgcctttt gctgccagaa acgctaccag ctctcccgct
     8521 gtcatccctt ctgccgagca ggtgtgaaaa cgtgcctgtt caccaaaacg cgctttaatt
     8581 gctgcttcca gactggcatg cgtatattgc tcgcctgatt caatcatcat atttaacact
     8641 tcatgaccgt gaatagagtc catcgttcct cctcaaaaaa agcctagcgt agcgattgcc
     8701 gcttatgaag actttgcgcc agagcagaac tgaatgcttt ttattgtaca tttatattta
     8761 caccatatgt aacgtcggtt tgacgaagca gccgttatgc cttaacctgc gccgcagata
     8821 tcactcataa agatcgtcag gacagaagaa agcgtgaaaa acagaactct gggaagtgtt
     8881 tttatcgtgg cgggaaccac aattggcgca ggcatgctgg caatgccgct ggctgcggca
     8941 ggtgttggtt ttagcgtcac gttaatcttg ttgattgggc tttgggcgtt gatgtgctac
     9001 acggcgctat tactgctgga ggtgtaccag catgttccgg cagataccgg tctgggcacg
     9061 ctggcaaaac gctatctggg acgctacggt caatggctga cgggcttcag tatgatgttc
     9121 ttaatgtatg ctctgactgc ggcatacatc agcggtgccg gtgaattgtt ggcctccagc
     9181 atcagcgact ggacaggtat ttcaatgtcg gcaaccgctg gcgtgctgtt gttcactttt
     9241 gttgccggtg gcgtggtttg tgttggaaca tcgctggtcg atttatttaa ccgttttctg
     9301 ttcagcgcca aaattatttt tctggtggtg atgctggtat tgctgctacc gcatattcac
     9361 aaagtgaatc ttttaaccct gccgttgcaa caggggctgg ctctgtcagc aatcccggtg
     9421 atttttacct catttggttt tcacggtagc gtgccgagta ttgtcagcta tatggatggc
     9481 aacgttcgta agctacgcag ggtgtttata accggtagtg cgatccccct ggtggcatat
     9541 attttctggc aggtggcaac gcttggcagc attgattcaa caacctttat gggattgctg
     9601 gctaatcatg ctggattaaa cgggctgtta caggcgttac gcgaaatggt ggcctctccg
     9661 catgttgagc tggcagtgca tttatttgct gatttagccc tcgccacgtc atttctcggc
     9721 gttgcgttag gcttatttga ttatctggct gatttatttc agcgttcaaa taccgttggt
     9781 ggacggttgc aaactggtgc aattaccttt ctgccgccgt tggcgtttgc actgttttat
     9841 ccacgaggat ttgtgatggc gctgggttac gccggtgtgg cgctggcggt actggcattg
     9901 attatccctt cgctgttgac ctggcaaagc agaaagcaca atcctcaggc gggttaccgg
     9961 gtcaaaggtg gtcgtccggc gctggtggtg gtgtttctct gtggtattgc tgtgattggc
    10021 gtgcaatttt tgattgcggc agggttgtta ccagaagtga ggtgatcaga tagcctcaaa
    10081 ttccttattg ggtgccagaa ttaacgctga cacccaattt ggcctcttaa tgcaggcagc
    10141 actgcttaaa tttcttacca ctaccgcacg ggcaaggatc gttacgcccc ggtttctctt
    10201 ctgctttgat cggttgctga acagcttttt cctgcggatg cgccatccag tacgcatgta
    10261 gatcaagcgc cgccagtcga atggcatcta cgctctcttc aaacgcttct ggcgacatct
    10321 tttcta
//
LOCUS       DAJTMC010000148        10291 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.148, whole genome shotgun sequence.
ACCESSION   DAJTMC010000148 DAJTMC010000000
VERSION     DAJTMC010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10291
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.148"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            119..748
                     /locus_tag="OO130_003313"
                     /pseudo
     CDS             119..748
                     /locus_tag="OO130_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004129194.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
     gene            745..1359
                     /locus_tag="OO130_003314"
     CDS             745..1359
                     /locus_tag="OO130_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HCR5366595.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALDRLIKEAARQDVAVTVLETET
                     GRYSDTLRSALVSLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(1416..2873)
                     /gene="pepD"
                     /locus_tag="OO130_003315"
     CDS             complement(1416..2873)
                     /gene="pepD"
                     /locus_tag="OO130_003315"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HCR5366596.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            3134..3592
                     /gene="gpt"
                     /locus_tag="OO130_003316"
     CDS             3134..3592
                     /gene="gpt"
                     /locus_tag="OO130_003316"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCR5366597.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3684..4928
                     /gene="frsA"
                     /locus_tag="OO130_003317"
     CDS             3684..4928
                     /gene="frsA"
                     /locus_tag="OO130_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HCR5366598.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIVMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            4986..5387
                     /gene="crl"
                     /locus_tag="OO130_003318"
     CDS             4986..5387
                     /gene="crl"
                     /locus_tag="OO130_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HCR5366599.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(5426..6481)
                     /gene="phoE"
                     /locus_tag="OO130_003319"
     CDS             complement(5426..6481)
                     /gene="phoE"
                     /locus_tag="OO130_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HCR5366600.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNDSKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGGEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAIGMTYQF"
     gene            6769..7872
                     /gene="proB"
                     /locus_tag="OO130_003320"
     CDS             6769..7872
                     /gene="proB"
                     /locus_tag="OO130_003320"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HCR5366601.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            7884..9137
                     /gene="proA"
                     /locus_tag="OO130_003321"
     CDS             7884..9137
                     /gene="proA"
                     /locus_tag="OO130_003321"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HCR5366602.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESVEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALAQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTCDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            9252..9327
                     /locus_tag="OO130_003322"
     tRNA            9252..9327
                     /locus_tag="OO130_003322"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9285..9287,aa:Thr,seq:cgt)
     gene            complement(9342..>10291)
                     /locus_tag="OO130_003323"
     CDS             complement(9342..>10291)
                     /locus_tag="OO130_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR5366603.1"
                     /translation="KKSLDDDKSRIGFWLQHFAGMQLRDITESKIYSAMQKMTNRRHE
                     ENWKLRAEACRKKGKPVPEYTPKPASVATKATHLSFIKALLRAAEREWKMLDKAPIIK
                     VPQPKNKRIRWLEPHEAQRLIDECPEPLKSVVEFALATGLRRSNIINLEWQQIDMQRR
                     VAWINPEESKSNRAIGVALNDTACRVLKKQIGNHHRWVFVYKESCTKPDGTKAPTVRK
                     MRYDANTAWKAALRRAGIDDFRFHDLRHTWASWLVQAGVPLSVLQEMGGWESIEMVRR
                     YAHLAPNHLTEHARQIDSILNPSVPNLSQSRNKERTNDV"
BASE COUNT         2465 a         2540 c         2683 g         2603 t
ORIGIN      
        1 ctattagcgg ttaccagcgc cccaccagac ccggtgacat caccccccgg atcatggacg
       61 actgggggca gtaatcatgc cgggtctggc tggctaacac cccattataa ggggtacgaa
      121 taaagtaacg gggcagtcta ttttacagcg gcatattgcc tcgttctcgc atcatggttt
      181 gcctcaaggc agtgacgacg cgctacggta tattgcggaa catgacgatg cgctggcgtt
      241 tgcgtgtatt aatcgccaga tgatcgcttt gcgcataatg caacaagtta aggccacggg
      301 aagtccggtt ctggatgtgg cgcataactt tgttagcgcg tgtcaaatcg gcgatcaaca
      361 gggctggttg catcgtaaag gtgccacgcc ggatgacaac ggtctggtga ttattcccgg
      421 ttcacgtggt gattactcct ggattgtaca gcctgttgcg aacgaaaaaa cgctccactc
      481 gctggcgcat ggggcagggc gtaaatgggg gcgcaccgag tgtaaatggc gtctggcagc
      541 gaaatacacg gtgacgcaac tctcgcggac tgaacttggc agtcggataa tttgtcgcga
      601 taaacaactc atctttgaag aagcgccaca agcctataaa tcggctgaaa gcgtggtgca
      661 atgtctggtg caggccggtt taattattcc tgtcgcgcgg ctgcgtccgg tgctaacgct
      721 caaaaacagt ggagggaaaa aaggatgatc ttgctacaac tctcctcagc gcaggggccg
      781 gaagaatgtt gtctcgcagt gaaaaaggcg ctggacaggc taattaaaga agctgcccga
      841 caagacgtcg cggtaacggt gttggaaaca gaaacgggcc gctactctga cacgctgcgt
      901 tcagctctgg tttctctgga tggcgataac gcatgggcat taagcgaaag ctggtgtggc
      961 actattcagt ggatttgtcc gagtccgtat cggcctcatc atgggcgcaa aaactggttt
     1021 ctgggcattg ggcgttttac cgctgatgag caggagcaat ccgatgcgat ccggtatgag
     1081 acgctgcgtt cgtcggggcc gggcggtcaa catgtcaata aaaccgactc ggcggtacgc
     1141 gccacgcatt tggcatccgg tattagcgtg aaggttcagt cagagcgtag tcagcatgct
     1201 aacaagcggc tggcacgatt gctgattgcc tggaagctgg agcagcagca acaggaaaat
     1261 agcgcggtgc tgaaatcgca gcggcgaatg ttccatcacc agattgaacg tggcaacccg
     1321 agacggacat ttacagggat ggcttttatc gaaggataat gaaggatgaa actgccggaa
     1381 ggcgattaaa cgccatccgg cagcaaatca aataattact tcgccggaat ttctttcagc
     1441 agttcagtca gcagtgtcca gtaatgacct acgctttcga tgtgaacttg ctcatccgga
     1501 gagtgtggac cggtgatagt tggcccgata gaaaccatgt ccatttccgg atacggtttt
     1561 ttgaacagac cacattccag gcccgcgtgg ataatctgga tgttcggcgt cttgttgaac
     1621 aggcgctgat aggtttcacg taccagatgc atcaccggag aattagcgtc cggctgccag
     1681 ccaggatatg cgcctttcgc ttcggttttc gcgccagcca gtttacccag cgaatccagc
     1741 atgctcacca cgtagtcttt accgctgtcg atcagtgaac ggatcaggca gtgaatttct
     1801 acgttattgt cagtcatggt caccacaccg acgttcaggg aggtttcaac cacacctttg
     1861 gctacatcgg agttgcggat cacaccgttc ggggtggcgt tcagcagacg aataaaggta
     1921 tcgcgagatt tcgcaatcag ggcagcttta tcgttcgcta cagagtccag caacaaggcc
     1981 agatttttct ctttctctgc cagctcgttt ttcaggatct cctgataggt attcaccaga
     2041 gatttcagtg cgtcgacttt atcagctgcg acagcaatgg tcgcaaaggc ttcacgcggg
     2101 atggcgttac gcagtgtgcc gccgttgaaa tcgataaggc gcaggtccag ttcttctgca
     2161 tgacccgcca ggaagcgcac cagcagtttg ttggcattac ccaggccaac gtggatttca
     2221 ccgccggagt gaccgccttt cagacctttt aaggttaact tgaaggtttc aaaaccagct
     2281 ggaaccgctt cacgatctaa atgcaggttg gaggtgaagt cgataccccc cgcacaaccc
     2341 atgtagattt caccttcttc ttcggagtcg gtgttaatca gaatatcagc ctgcaaccag
     2401 ttgctctgta agccaaaagc accgtccata ccggcttctt cggtcatggt cagcagcact
     2461 tccagcggac cgtgaaccac gttttcgtca gccagaaccg ccagtgcaga ggccatacca
     2521 atgccgttat ccgcacccag cgtggtgccg cgcgctttaa cccattcgcc atcaatataa
     2581 ggctggatag gatctttcgt gaagtcatgc acggtgtcgt tatttttctg cggcaccata
     2641 tcgaggtggg cctgtaagac gaccggttta cgattttcca tacctgcggt agcaggttta
     2701 cgaatcagga tattacctac ctgatcgcgt tcgacatgga aacctttctc ttttgcccaa
     2761 ccaacaatgt attcagcgag ttgctcttca tgataggacg ggtgcggaat agaacagatt
     2821 ttggcaaaaa tatcccacag cggctgtgga gataattgag acagttcaga cacgttaagt
     2881 ctccttgtcg atcacccgca aaacagtatt gcaggtcaca gggttagcag aaaatgttgt
     2941 caacacaaga caggcttgcg agatatgttt gagaatacca ctttatcccg cgtcagggag
     3001 aggcagtgcg taaaaagacg cggactcatg tgaaatactg gtttttagtg cgccagatct
     3061 ctataatctc gcgcaaccta ttttcccctc gaacactttt taagccgtag ataaacaggc
     3121 tgggacactt cacatgagcg aaaaatacat cgtcacctgg gacatgttgc agatccatgc
     3181 acgtaaactc gcaagccgac tgatgccttc tgaacaatgg aaaggcatta ttgccgtaag
     3241 ccgtggcggt ctggtaccgg gtgcgttact ggcgcgtgaa ctgggtattc gtcatgtcga
     3301 taccgtttgt atttccagct acgatcacga caaccagcga gagcttaaag tgctgaaacg
     3361 cgcagaaggc gatggcgaag gcttcatagt tattgatgac ctggtggata ccggtggtac
     3421 tgctgttgcg attcgtgaaa tgtatccaaa agcgcacttt gtcaccatct tcgcaaaacc
     3481 ggctggtcgt ccgctggttg atgactatgt tgttgatatt ccgcaagata cctggatcga
     3541 acagccgtgg gatatgggcg tcgtattcgt cccgccaatc tccggtcgct aatcttttca
     3601 acgcctggca ctgccgggcg ttgttctttt taacttcagg caggttacaa tagtttccag
     3661 taagtattct ggaggctgca tccatgacac aggcaaacct gagcgaaacc ctgttcaaac
     3721 cccgctttaa acatcctgaa acctcgacgc tagtccgccg ctttaatcac ggcgcacaac
     3781 cgcctgtgca gtcggctctt gatggtaaaa ccatccctca ctggtatcgc atgattaacc
     3841 gtctgatgtg gatctggcgc ggtattgacc cacgtgaaat cctcgacgtc caggcacgta
     3901 ttgtgatgag cgatgctgaa cgtaccgacg atgatttata cgatactgtg attggctacc
     3961 gtggtggcaa ctggatttat gagtgggcca cccaggcgat ggtgtggcaa caaaaagcct
     4021 gtgcggaaga agatccgcaa ctcagtggtc gacactggct gcatgcggct acgttgtaca
     4081 acattgccgc ctatcctcat ctgaaaggag atgacctggc cgagcaagcg caggctttat
     4141 caaaccgcgc ctatgaagag gccgcccagc gtctgccggg cacgatgcgg cagatggagt
     4201 ttaccgtacc cggcggtgcg cccatcaccg gctttttgca tatgccgaaa ggcgatggcc
     4261 cgttcccgac agtattaatg tgtggtggtc tggatgcgat gcagacggac tattacagcc
     4321 tgtatgaacg ttattttgcg ccgcgcggca ttgtgatgct gactattgat atgccgtcgg
     4381 tgggcttttc ttcaaaatgg aagctcaccc aggactccag cctgttgcat cagcacgtct
     4441 taaaggcgct gcctaacgta ccgtgggtgg atcacactcg cgtcgcggcc tttggtttcc
     4501 gtttcggcgc taacgttgcc gtgcgtctgg catatcttga atcgccgcgt ctgaaagcgg
     4561 ttgcctgtct tggtccggta gttcataccc tgttgagtga ttttaagtgc cagcaacagg
     4621 tgccggaaat gtatcttgac gttctggcga gtcgtttggg gatgcatgat gcttccgacg
     4681 aagcgttacg cgtggagctg aatcgctatt cattaaaagt gcaaggattg ctgggacgtc
     4741 gctgcccaac gccaatgtta tcaggctact ggaagaacga tccgttcagc ccggaagagg
     4801 actcacgctt aatcacctca tcatctgctg acggtaaatt attagagatc ccatttaacc
     4861 cggtgtatcg gaattttgac aaaggtcttc aggaaatcac cggctggatc gaaaaacgct
     4921 tgtgttaaaa atttgctaaa ttttgccaat ttggtaaaac agttgcatca caacaggaga
     4981 tagcaatgac gttaccgagt ggacacccga agagcagatt gatcaaaaaa tttaccgcac
     5041 taggcccgta tattcgtgaa ggtaagtgcg aagataatcg attctttttc gattgtctgg
     5101 ctgtatgcgt caacgtgaaa ccggcaccgg aagtgcgtga attctggggc tggtggatgg
     5161 agctggaagc gcaggaatcc cgttttacct acagctacca gtttggtctg ttcgataaag
     5221 caggcgactg gaagagtgtt ccggtaaaag atactgaagt ggttgaacga ctggagcaca
     5281 ccctgcgtga gtttcacgag aagctgcgtg aactgctgac gacgctgaat ctgaagctgg
     5341 aaccggcgga tgattttcgt gacgagccgg tgaagttaac ggcgtgagtg aaatgtgccg
     5401 gatgcatcac atccggcaat atctattaaa actgatacgt catcccaatc gcgacaatat
     5461 catcattatt aatattcaat ttgttatcgc tatccagttg gttgatttta taatcaacaa
     5521 atgctgacat atttttgttg aaataatacg tagcaccgac gtcgatataa ttaaccagat
     5581 cttcaccacc gatgccctca atatctttcc ctttcgataa gacataaccc agcgatggac
     5641 gcagaccaaa gtcaaactgg tattgagcga ccgcttcaaa gttctgtgtc ttattggcaa
     5701 agccgccagt tattggcgtc attttgcgag tttcagaata gaaagttgcc agataaatat
     5761 tattggcatc gtatttcaga cctgttgccc atgcttctgc acgcttgcct gtgccacggc
     5821 tttgcaggtt ctgctcgttg gtgcgatctg agttggtata ggccccacta atggcgaaat
     5881 cgctgccgcc aaagtcatat gtcaatgacg tgccgaagcc atcgccgttt tgctttttaa
     5941 cgtcgcggtt ttcgtttttc ccttgatatt gcagggttaa gttcaggcca tcgataacgc
     6001 cgaagaagtc ggtgttccga tacgtcgcca gaccgctggc gcgtttggtc ataaagttgt
     6061 cggtctgcgc cgaggagtcg ccaccaaatt ccggaaacat atcggtccag gcttccacgt
     6121 catacaacgc gcccaggtta cgaccataat cgaaagaacc caaatcttta tatttcaacc
     6181 cggcaaaagc gagacgcgtt ttttgctgtg cagtatcact ctctgcttta ttaccggcaa
     6241 actctgcttc ccaacgacca taaccagtca gttgatcgtt aatttgtgtt tcgcctttaa
     6301 aaccaaaacg gatataactc tggtcgccat ctttactgtc gttatcactc atataatgca
     6361 tggctttaac tttgccatag acatccagtt tattaccgtc tttgttatat atttctgcgg
     6421 cctgtacgga tgcagatgcc acaatgccca tcaccactaa tgccagagtg ctctttttca
     6481 ttttcattcc tgattttaat taacgcgcga atattcagcg ggagagcccc gttgaaaaca
     6541 ggaaagtttt taacctgaga ttgttaaaga tatattacag attaataata ttcttaaaat
     6601 gtggtaattt attaaatctg taataaaagc gtaaacaact gccgctaggc ttgctgatcc
     6661 cgcgcaacaa aacgccatgc tttgctcgca gatggttggc aaccgacgac agtcctgcta
     6721 aaacgttcgt ttgatatcat ttttcctaaa attgaatggc agagaatcat gagtgacagc
     6781 cagacgctgg tggtaaaact cggcaccagt gtgctaacag gcggatcgcg ccgtctgaac
     6841 cgtgcccata tcgttgaact tgttcgccag tgcgcgcagt tacatgccgc cgggcatcgg
     6901 attgttattg tgacgtcggg cgcgatcgcc gccggacgtg agcacttggg ttacccggaa
     6961 ctgccagcga ctatcgcctc gaaacaactg ctggcagcgg tagggcagag tcgactgatt
     7021 caactgtggg aacagctgtt ttcgatttat ggcattcacg tcgggcaaat gctgctgacc
     7081 cgtgctgata tggaagaccg tgaacgcttc ctgaacgccc gcgacaccct gcgtgcgttg
     7141 ctcgataaca atatcgttcc ggtaatcaat gagaacgatg ctgtcgctac ggcagagatt
     7201 aaagtcggcg ataacgataa cctttctgcg ctggcggcga ttctggcggg tgccgataaa
     7261 ctgttgctgc tgaccgatca aaaaggtttg tataccgctg acccgcgcag caatccgcag
     7321 gcagaactga ttaaagatgt ttacggcatt gatgacgcac tgcgcgcgat tgccggtgac
     7381 agcgtttcag gcctcggaac tggcggcatg agtaccaaat tgcaggccgc tgacgtggct
     7441 tgccgtgcgg gtatcgacac cattattgcc gcgggcagca agccgggcgt tattggtgat
     7501 gtgatggaag gcatttccgt cggtacgctg ttccatgccc aggcgactcc gcttgaaaac
     7561 cgtaaacgct ggattttcgg tgcgccgccg gcgggtgaaa tcacggtaga tgaaggggca
     7621 actgccgcca ttctggaacg cggcagctcc ctgttgccga aaggcattaa aagcgtgact
     7681 ggcaatttct cgcgtggtga agtcatccgc atttgcaacc tcgaaggtcg cgatatcgcc
     7741 cacggtgtca gtcgttacaa cagcgatgca ttacgccgta ttgccggaca ccactcgcaa
     7801 gaaattgatg caatactggg atatgaatac ggcccggttg ccgttcaccg tgatgacatg
     7861 attacccgtt aaggagctgg ccgatgctgg aacaaatggg cattgccgcg aagcaagcct
     7921 cgtataaatt agcgcaactc tccagccgcg aaaaaaatcg cgtgctggaa aaaatcgccg
     7981 atgaactgga agcacaaagc gaaatcattc tcaacgctaa cgcccaggat gttgctgacg
     8041 cgcgagccaa tggccttagc gaagcgatgc ttgaccgtct ggcactgacg cccgcacggc
     8101 tgaaaggcat tgccgacgat gtacgtcagg tgtgcaacct cgccgatccg gtggggcagg
     8161 taatcgatgg cggcgtactg gacagcggcc tgcgtcttga gcgtcgtcgc gtaccgctgg
     8221 gagttattgg cgtgatttat gaagcgcgcc cgaacgtgac ggttgatgtc gcttcgctgt
     8281 gcctgaaaac cggtaatgcg gtgatcctgc gcggtggcaa agaaacgtgt cgcactaacg
     8341 ctgcaacagt ggcggtgatt caggacgccc tgaaatcctg cggcttaccg gcgggtgccg
     8401 tgcaggcgat tgataatcct gaccgtgcgc tggtcagtga aatgctgcgt atggataaat
     8461 acatcgacat gctgatcccg cgtggtggcg ctggtttgca taaactgtgc cgtgaacagt
     8521 cgacaatccc ggtgatcaca ggtggtatag gcgtatgcca tatttacgtt gatgaaagtg
     8581 tagagatcgc tgaagcatta aaagtgatcg tcaacgcgaa aactcagcgt ccgagcacat
     8641 gtaatacggt tgaaacgttg ctggtgaata aaaacatcgc cgatagcttc ctgcccgcat
     8701 taagcaaaca aatggcggaa agcggcgtga cattacacgc agatgcagct gcactggcgc
     8761 agttgcaggc aggccctgcg aaggtggttg ctgttaaagc cgaagagtat gacgatgagt
     8821 ttctgtcatt agatttgaac gtcaaaatcg tcagcgatct tgacgatgcc atcgcccata
     8881 ttcgtgaaca cggcacacaa cactccgatg cgatcctgac ctgcgatatg cgcaacgccc
     8941 agcgttttgt taacgaagtg gattcgtccg ctgtttacgt taacgcctct acgcgtttta
     9001 ccgacggcgg ccagtttggt ctgggtgcgg aagtggcggt aagcacacaa aaactccacg
     9061 cgcgtggccc aatggggctg gaagcactga ccacttacaa gtggatcggc attggtgatt
     9121 acaccattcg tgcgtaaata aaaccgggtg atgcaaaagt agccatttga ttcacaaggc
     9181 cattgacgca tcgcccggtt agttttaacc ttgtccaccg tgattcacgt tcgtgaacat
     9241 gtcctttcag ggccgatata gctcagttgg tagagcagcg cattcgtaat gcgaaggtcg
     9301 taggttcgac tcctattatc ggcaccattt aaatcaataa gttacacatc attagtacgt
     9361 tccttatttc ttgactggga caaatttggg accgatgggt tcagaatcga gtctatttgc
     9421 cgtgcgtgtt cggtaaggtg attaggtgcg aggtgagcat atcgacgaac catttcgata
     9481 gactcccagc ctcccatttc ctgtaacact gataacggga ctccggcttg aaccagccaa
     9541 cttgcccagg tgtgtctcaa gtcgtgaaat ctgaaatcat caatacctgc tcgtctcagc
     9601 gccgctttcc aggctgtgtt tgcgtcatac cgcatcttcc tgactgttgg cgctttcgtt
     9661 ccgtctggtt tggtacagct ttccttatac acaaataccc aacggtgatg attcccgatt
     9721 tgttttttca atacgcgaca tgcagtatca ttcagcgcaa cgccaattgc gcggtttgat
     9781 ttactctctt ccgggtttat ccatgccacc cggcgctgca tgtctatttg ttgccattca
     9841 aggttgatga tgttcgagcg tcttaagcct gttgcaagtg caaattcaac aacagacttt
     9901 aatggctccg gacattcatc aatcagcctt tgtgcttcat gaggctccag ccagcggatc
     9961 cgtttattct ttggttgagg cactttaata attggtgcct tatccagcat tttccattca
    10021 cgctctgcgg ctcttagcag ggcctttata aatgaaagat gcgtagcctt cgttgcaacg
    10081 gacgctggtt ttggcgtgta ttctggaaca ggtttccctt tttttctgca tgcttctgcc
    10141 ctgagtttcc agttttcctc atgacgccgg ttcgtcattt tctgcattgc tgaataaatt
    10201 tttgattcag taatgtctct tagttgcatt cctgcgaaat gttgaagcca gaatccgatc
    10261 cggcttttgt catcgtccag tgatttttta t
//
LOCUS       DAJTMC010000149        10243 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.149, whole genome shotgun sequence.
ACCESSION   DAJTMC010000149 DAJTMC010000000
VERSION     DAJTMC010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10243
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.149"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            <1..236
                     /locus_tag="OO130_003324"
     CDS             <1..236
                     /locus_tag="OO130_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5366604.1"
                     /translation="TLVEEDKALLIGNGLKLRLLDENASPYTFNKYAEYADFTSDMLV
                     YEKTYTAELSSIAGTPIEAGPFDTVVLFKINYN"
     gene            388..1434
                     /locus_tag="OO130_003325"
     CDS             388..1434
                     /locus_tag="OO130_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCR5366605.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTCM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPDGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(1431..2222)
                     /gene="nei"
                     /locus_tag="OO130_003326"
     CDS             complement(1431..2222)
                     /gene="nei"
                     /locus_tag="OO130_003326"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HCR5366606.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVGTRGKALLTHFSNDLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(2258..2992)
                     /gene="pxpA"
                     /locus_tag="OO130_003327"
     CDS             complement(2258..2992)
                     /gene="pxpA"
                     /locus_tag="OO130_003327"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HCR5366607.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWATVTAQTVCLH
                     GDGEHALAFARRLRSTFAEKEIVVAA"
     gene            complement(2982..3914)
                     /gene="pxpC"
                     /locus_tag="OO130_003328"
     CDS             complement(2982..3914)
                     /gene="pxpC"
                     /locus_tag="OO130_003328"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HCR5366608.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLNVGIGGLEGRLLKDGDRLPIGKSKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSPQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            complement(3908..4564)
                     /gene="pxpB"
                     /locus_tag="OO130_003329"
     CDS             complement(3908..4564)
                     /gene="pxpB"
                     /locus_tag="OO130_003329"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HCR5366609.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIELLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAHCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            complement(4587..5330)
                     /gene="ybgI"
                     /locus_tag="OO130_003330"
     CDS             complement(4587..5330)
                     /gene="ybgI"
                     /locus_tag="OO130_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HCR5366610.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            5601..7082
                     /gene="dtpD"
                     /locus_tag="OO130_003331"
     CDS             5601..7082
                     /gene="dtpD"
                     /locus_tag="OO130_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HCR5366611.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(7124..8542)
                     /gene="phrB"
                     /locus_tag="OO130_003332"
     CDS             complement(7124..8542)
                     /gene="phrB"
                     /locus_tag="OO130_003332"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HCR5366612.1"
                     /translation="MTTHLVWFRQDLRLHDNLALAAACSNSSARVLALYIATPRQWAT
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVEVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIEPSPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHCPFIAWTDRVQWQSNPAHLQAWQEGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPTTQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVMLDYPQP
                     IVDHKEARLRTLAAYEEARKGA"
     gene            complement(8539..9048)
                     /locus_tag="OO130_003333"
     CDS             complement(8539..9048)
                     /locus_tag="OO130_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HCR5366613.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDYYNQYRQRVVVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            complement(9333..9707)
                     /locus_tag="OO130_003334"
     CDS             complement(9333..9707)
                     /locus_tag="OO130_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5366614.1"
                     /translation="MFTKLSIAILLAFSAYVHAETSAPIFFEDVGSIDSWFSIKYQKY
                     ENVKDEQYPEILIYYGVKGSGINRVDGWFFSCAKAGRTCSLIFMADFGESRYFKPAKI
                     AMNKNNLIIRTSDVEIAFKITK"
     gene            complement(10166..>10243)
                     /locus_tag="OO130_003335"
     CDS             complement(10166..>10243)
                     /locus_tag="OO130_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HCR5366615.1"
                     /translation="SLSFSKSVELHDKVIGHYLNIKHYQ"
BASE COUNT         2390 a         2775 c         2460 g         2618 t
ORIGIN      
        1 aaacgttggt tgaagaagat aaagcattgt tgattggtaa tggtctgaag ctacgtttgc
       61 tggatgaaaa cgcctcgcct tataccttca ataaatatgc tgaatacgcc gatttcacca
      121 gcgacatgtt ggtctacgaa aaaacctata cagctgaact ttcgtccatt gcaggtaccc
      181 ccatcgaggc tgggcccttc gatacagtgg tgctttttaa aattaactat aactgatttg
      241 ccatgatgcc ctttgcagcc ggaaagggca tctctttcag aaaattgctg attttcctct
      301 tttccttgat tactgttatt ttttcctggc ccggctttct ttatgctgta tcgatacacc
      361 aaagagaaaa taatgaggga gcgaaggatg ccagttttgc agtggggaat gttatgtgtg
      421 ctatcacttc tcctttctat tggtttcctc gcggtacatc tcccggcggc gctattactt
      481 gggccgatga tcgccgggat catcttcagt atgcgcggaa ttactctgca acttccccgc
      541 tccgcttttc tcgccgcgca ggccattctt ggctgcatga ttgcgcaaaa cctcactggt
      601 tcgattctca ccacgctggc cgccaactgg ccgatcgtgc ttgctatttt actggtgacg
      661 ctgctttcca gcgccatcgt gggttggtta ttggtgcgct atagctcact gcctggaaat
      721 accggtgcct ggggttcttc tccaggcggc gcagcggcaa tggtcgctat ggcgcaagat
      781 tacggcgcag atattcgcct ggtggcgttt atgcaatatc tgcgagtgct gttcgtcgcg
      841 ggggccgctg ttctggtcac ctgcatgatg ctgggtgata acgctgaagc ggttaaccag
      901 caaatcgtct ggttcccgcc agtgagcatt aatctcctgc ttacgatttt gctcgcagtc
      961 gttgccggta cggcgggatg catgctgcgt cttccttccg gcacgatgct catcccgatg
     1021 ctggcgggcg cagtgctcca gtctggtcag ctcatcacca tcgaactacc ggaatggctg
     1081 ctggcgatgg cgtatatggc aattggctgg cggattggtc ttggtttcga taaacaaatc
     1141 ttactgcggg cactacgccc actaccgcaa atcctgctgt cgatttttgc tctgctggct
     1201 atttgtgcag gtatggcgtg gggactgacc cggtttatgc atattgattt tatgactgcc
     1261 tacctcgcca ccagtcccga cgggcttgat accgtggcgg tcatcgccgc agggagcaat
     1321 gccgatatgg cgcttattat ggcgatgcaa accctgcgcc tgttcagtat tttgctgacg
     1381 gggccagcca ttgcgcggtt tatttcaacc tatgcgccga agcggtcggc ctagtgctgg
     1441 cagcctggac accagtaaaa cgggcgcgag gacaacgtgg ttttctcaat gatgccgcca
     1501 cagcgttcgc acagttcgcc atcacgatga aaaaccttaa agcgaaacag cgccccatga
     1561 tgcttattct catccacctg cccccgcgta gcgtaagaaa accgaggaat ctccagtaac
     1621 gcgtgtgcga gtgcatccag ttgcgccgca ttgagatctt tcgctttatg atttccagtc
     1681 aacccaacct gccagaggat ctccacccgc aaataattgc caagcccagc cagaaacgcc
     1741 tgatcgagca gtaatcctgc aaactgacgg ttacgaaagc gcggtgacaa taaccgttcc
     1801 tttaccacct ccggcgtcag attcggatcc agcacatcgg gaccaacgcg ttgtaaaaac
     1861 ggatgcgtgg tcagttgttc cggcctcaac atctcaatat ccgaggcgct ataaagcaga
     1921 atggttttgt cagccgtttg cagttttacc cgcaatactc gcgtggtctg cggctcttcg
     1981 ccggtatcaa ccacgcgcca gacgccgtaa agctgattat ggctgtagag cgttaagtcg
     2041 ttggaaaaat gagttaacaa cgccttacca cgcgttccca catgggtaac gtgttgaccg
     2101 ataagttgtg attgataagt ttttaactgc gggaaggcaa accagacatc agttagtggt
     2161 ttgcctttga tcgccgcctc caggttatcc gctgcacggc ggatctccgg gccttcaggc
     2221 atggtgatat ccttttttca ttctttatta atcggggtta tgctgcgaca acaatctcct
     2281 tttcggcaaa tgtagagcgt agtcggcggg cgaacgccag tgcgtgctcg ccgtcgccat
     2341 gcaggcagac tgtttgcgcc gtgaccgttg cccattcgcc ggtgatactt ttgactctgc
     2401 cgtgttgcac catctctagc gtttgcgcca gcgcctgttc ttcgttttca atcaacgcgc
     2461 ctggctgact tcgcggcacc agcgagccgt cagcctgata accgcgatcg gcaaacactt
     2521 cctcgcgcgt tgtcaggcca tattgcttgc ctgcacgaat cagctcgctt cctgccagcc
     2581 caacgagaac caatgctgga tcgcaagcgt ataccgctct ggcgatggcg tctgccagtt
     2641 gtgcttcttt cgccgcctgg ttgtacaaca tgccgtgcgg tttgacatga cgcattacgc
     2701 cgccttgcgc acgggcaatc gttgccagcg cgccaatttg atacagcgtc tgggcgtaaa
     2761 cggtttctgg cggcagctgc atggcgctgc gaccaaaatt ttccctgtcg ggaaaactcg
     2821 ggtgagcgcc aatcgcgaca ccatttttta ttgcttcacg cacgcaagcc tgcatggttt
     2881 gggcatcgcc tgcatgaaat ccacaagcaa tattggcaga ggaaaccagc gttaatagct
     2941 ctgcgtcgct ggcgcagcct tcgcccagat cggcgttcag gtcaattttc attgtgtagc
     3001 cgccacgcta attgttcgaa ataacgttgt tgatcttgcc gcgctttcag tgcctcttcc
     3061 agtgaacact ggacaaaatg aatcggctga ccgagcggaa tttgcgccag atggtacata
     3121 tcagcctcaa tgatacaggc aatacgcggg taaccaccgg tggtctgtgc gtcgttcatc
     3181 aacacaatcg gctgcccgtt atgcggcacc tgcaccacac ccggtaacaa accgtgagat
     3241 aacagttcgc gatcggtggt gcgttttaaa atttgcccct gtaagcgata gcccatgcgg
     3301 ttactttgcg ggctaagctg ccagggcgaa cgccagaatg catcctgcga ggcgcgatcg
     3361 aactcatgat attccggccc cggcaaggcg cgaatgcggt tgccccacag cagctgttta
     3421 acgccctgcg cttccataaa atcacgcttc gatttgccaa tcgggagtcg gtcaccatcc
     3481 ttcagtaaac ggccttccag cccgccaatc cccacgttga gatcggtgct gcatgacccc
     3541 attaccggcg gaacatcaat accacccgcg accgccagat aactgcgcat gccgtgctgc
     3601 gggcgtttaa gcgttaaacg ctggcctgct ttcatcggca agcgccagcc ggtccagacg
     3661 gcgttatcat ccagccgcgc ttcgcaaccg gcacccgtca gagcaaacca cccatcagtt
     3721 tcgaactcaa ccgttaactg accgagcgtg atctccagcg cgggggcatt ggcgtcatta
     3781 cccaccagca ggttagcaat gcgtaacgca ggcatatcca gtgcgccgca gtggctgata
     3841 cccgactggc gaaaaccgtg acgaccgcca tcctgcacag tggtatacat gcccgcacga
     3901 ataatcttca gcatactccc tccttctgcg gtacaaagcg cacgctgtct cccggacgta
     3961 ataagatggg ttcgtcacgc gccggatcaa acaggctgag tgaggtatga ccaatcaact
     4021 gccagccacc cggcgttgcc agcggataaa caccagtctg cggcccgccg atcccgacag
     4081 aacctgccgg aacgagcaag cgcggttcag cgcgccgtgg cgtgtgtagt tgttccggca
     4141 aactcccgag atacgggaag cccggttgaa aacctaaaaa ccagaccacg tattccacgg
     4201 aggagtgcaa ttcaacaacc tgtttttcgc tcaacccgca atgcgccgcg accaccgcca
     4261 aatccggtcc gcctgcacca ccgtaaacca ccggaatttc aataaagcga gactcaggtt
     4321 ccagggcctc gctctcctcc caccagcgtt gcaaaagctc tatggcatcc agcgccagcg
     4381 actcaggatt acgcaaaatc accgtgatat tgttcatgcc gggaatggct tcaaccacat
     4441 tcggcatatc caccagacgc tgcgccagtc gccagatccg tttctggcta gccagcgtca
     4501 ccggcggttc cagttccagc actaccgccg tttcacctat cagataacaa cgcgctcgtt
     4561 gcactttcga tccctctgat tattcgttat gcaggattag gaatatcaat aaaggtcaca
     4621 tcaagatcgg tattttcatt cagccactcg ctcaatgcgc gaataccacc acgttcagtg
     4681 gcatggtgac ctgcagcata aaaatgcaat ccctgctcgc gggctgaatg aatggtctgt
     4741 tcagaaactt cgccagtaat aaaagcatcc acgccaaaac gcgcggcgct atcgataaaa
     4801 ctttgcccgc cgcccgtgca ccaggcgacg cgctggacca cctcaggtcc ggtatcgcca
     4861 caccataacg gcttacgtcc cagacgcgct tcaatccagg aggctagttc cagtcccggc
     4921 acaggcatgg tcagctctcc ccacggcacc aacggctcaa tttcgcccat gaccgtgatc
     4981 cccagtaacg ccgccagctg tgcgttattg cccagctcag gatgtgcgtc aagcggcaaa
     5041 tgccagccat acaggttgat atcattcgcc agcaacgttt ttaaacggtt acgcttcatt
     5101 ccgcgaatga ccggagactc tcctttccag aagtagccat gatgcacaat gaccgcatcc
     5161 gcgcccagac gcactgcctc atcgagcaaa gcctggctgg cggtgacacc ggtaacaatt
     5221 ttttgcaccg tctctttgcc ttccacctgc aaaccattcg gcgcgtaatc actaatcgcc
     5281 gcgctgttca gtttttcgtt aatcagttgt tccagttcgg tgtttttcat cttcgcgcct
     5341 tccgtattct gatagctaca ccgttaacat agcgttacct cgcctgttcg tcgatagcca
     5401 atcttttgac atattaagaa acaaacccga cgccaaaaga cgcaaattgt ttatatcatc
     5461 acgcttcagc taatgtatat ttatgcaatc agacagcata ttgcataaat aatcagatct
     5521 gtatcaaagt tttaacttta aggaaacaca agcacgccgc gcaactatta cgtgacgttg
     5581 ccgtcaattg tggaattatt atgaataaac acgcatcaca gccgcgcgct atttactatg
     5641 tcgttgcgct gcaaatctgg gaatatttta gcttttacgg catgcgtgcc ctgctgattc
     5701 tctatctcac caatcaacta aaatacaacg atactcacgc ctacgagtta tttagcgcct
     5761 actgttcgct ggtgtatgtc acgccaatcc tcggtggctt tttggcggat aaagttctcg
     5821 gcaatcgcat ggcggtgatg ctgggggcgt tattgatggc gatcggtcat gtggtgttgg
     5881 gtgccagtga gatccatccg tcattcctct atctgtccct ggcgataatt gtttgtggct
     5941 atggtctgtt taaatccaat gtcagttgcc tgctcggcga gctgtatgag ccaaccgatc
     6001 cgcgtcgtga tggcggtttc tcgctgatgt atgcagcggg taacgtgggg tctattatcg
     6061 cacctattgc ctgtggctac gcccaggaag agtacagctg ggcgatgggc tttggcctgg
     6121 cggcggtggg tatgatcgcg ggtctggtca ttttcttatg tggcaatcgt catttcacac
     6181 atacccgcgg cgttaataaa aaagtactgc gtgcgacaaa ctttctcctg cctaactggg
     6241 gatggctgct ggttctgctg gtggcaacgc ctgcgctgat taccgtattg ttctggaaag
     6301 agtggtcggt atacgcctta attgtcgcga ctattattgg cctgggtgta ctggcaaaaa
     6361 tttatcgcaa agcagaaaac cagaaacagc gtaaggagct ggggctgatt gtgacgctca
     6421 ccttcttcag tatgttgttc tgggccttcg cacaacaggg cggcagctcg attagccttt
     6481 atatcgaccg cttcgttaac cgcgatatgt ttggttatac cgttccgact gctatgttcc
     6541 agtcgattaa tgccttcgca gttatgctgt gcggtgtgtt cctggcgtgg gtggttaaag
     6601 aaagcgtcgc aggtaatcgt accgtgcgca tctggggaaa atttgctctt ggtctcggcc
     6661 tgatgagcgc cgggttctgc attctgacct taagcgcccg ctggtccgca atgtatggtc
     6721 actcttctct gccactgatg gtattaggcc tggcggtgat gggctttgcg gaactgttta
     6781 tcgacccggt tgccatgtcg caaattacgc gcattgaaat ccccggtgtg accggcgtat
     6841 taaccggcat ttatatgctg ctttccggcg cgattgcgaa ctatctcgcg ggcgtgattg
     6901 ccgatcagac atcgcaggct tcgtttgatg cttccggggc gatcaactac tccatcaatg
     6961 catatattga agtatttgat caaattacct ggggcgcact ggcgtgtgta ggactggtac
     7021 tgatgatttg gctgtatcag gcgctgaaat tcagaaaccg cgcgctggcg ctggagtcat
     7081 aataggccag caccatacct gataaaacgc tcgctgcgtt ttatcaggct cctttcctcg
     7141 cttcttcgta cgctgccagc gttcgcagcc ttgcctcttt gtgatcaact atcggttgcg
     7201 gataatccag catcacacct gctttctgcg cccacttcca cggctcatgc accgctttcc
     7261 ctggtacatc gcgcagctcc ggtaaccatc gacgaataaa ctcgccctca cggtcaaatt
     7321 tctcgccctg ggttgtcggg ttgaaaatac gaaaatacgg cgctgcatcg gttccggttg
     7381 aagcggccca ctgccagcca ccgttattgg ctgccaaatc accatcaatc agctgcgaca
     7441 tgaaatatcg ctcgccttcg cgccagtcga tcaacaaatc tttcaccaga aaactggctg
     7501 taatcatccg tagccggtta tgcatccagc cagtgctgtt aagctgacgc atggcagcat
     7561 cgacaatcgg gtatcccgtt ttgccttcct gccaggcctg taaatgtgcg ggattgctct
     7621 gccactgcac acgatccgtc caggcaataa acggacaatg tttacacaac gaggggtaat
     7681 acgtcatcag atgacggtag aactcgcgcc agatcagctc actaagccag acactaccgg
     7741 ccccaccgtc cagcgcctgc ggctgttccg ccaacaagcg atgcaaacac tggcgaggcg
     7801 ataacccgcc cgttgccagg ctggcggaca aacgactggt gccttccact gccggaaaat
     7861 ctcgttgttg ctcatattct ccggcaccgt tctggcaaaa ctggcgtaat tgcgcaatcg
     7921 ccgctttttc ttccaccggg aaatgcgcag tatcgaaaga ctgacgagga taattcagcg
     7981 taatggatgg cgagggctct atcgatccgc tactacgaac ttttggcgca gcgacgcact
     8041 ccggcatccc ttcccgcagc cgtttcagcc aggcattctt aaaaggcgta aagactttgt
     8101 acatctcgtg attaccggtc atcaccgcgc caggcggcag gatcacgctg tcatcaaatc
     8161 cttcacacac cacgttacgc agcgctcttt caacttccac atcccgcgcc cgctcattca
     8221 cttcatactg atagttataa aacaggtggg taacgctgtt ttccgcgcac acctgtttaa
     8281 ctatttcgac actggcgaca aagtcatcca cttcacggaa caataaagga ataccttttt
     8341 ccgcaagcgc tatttgtagc ccattcagtt gagcattgat aagttcagcc tgacgcggcg
     8401 acatgttatg cgtcgcccac tggcgtggtg tagcgatata caacgccagc acgcgtgcag
     8461 acgaattgct gcaggcggca gccagtgcga gattatcgtg caaacgtaaa tcctggcgaa
     8521 accagaccag atgggtagtc ataaaactcc tgaatggcgc aagttaatca cacgcggcca
     8581 aagttcgaaa taacgctgcc ctgaaagcca ggcgtcagga taaagcgcca tatagtgttt
     8641 gatacgcatc agcggcgcaa gaagtggttg ctcgccacgg cgataactgt cgataagcgc
     8701 agccagctgc tggcgttctg tggaatcaat atgcgggcga aaataaccct gaacgtgcat
     8761 caaaacattg gtgtgatcgc gcgggttggc ggggtgagaa agcaaaacaa ctacgcgttg
     8821 gcggtactgg ttgtaatagt catccagatt ttgccactgg tgaatatcag ccacgaacgg
     8881 gccaagtttg cggtactccg gctgagaatg cgccagaaag acgagtttat agcggctgtg
     8941 ataatcgaga agcgccccgc gcgttaagcc atgttctttc agtcggtgta actcatgaag
     9001 ggcaaagata cggattaggg tgctgtcatc aaatcgttgt agattcatta ctgctcctga
     9061 acggtataaa ggagtaagtg tagacgatgg taaatgaatt ttatggggca gagtttttgc
     9121 ggggaagtat tttgttggta aaaatattat caacttcatc tgagcgagaa ttattctact
     9181 atttcccgag ctgcattcat tttttgtatt ttttattttt agaaaataac agttctcaaa
     9241 gcttaattac ctcactatta attgtatttc aaactaacaa tcgcactcca ttttctctct
     9301 ttaaaaaaca taaacactgt taaaacaaat atttatttcg ttatcttaaa tgctatttct
     9361 acatcactcg tccttataat caaattattc ttattcatag ctatttttgc aggtttaaaa
     9421 tatcggcttt caccaaagtc agccatgaaa attagactac aagttctccc agcctttgca
     9481 catgagaaaa accacccatc aactctattt attcctgaac ctttaacacc ataataaatc
     9541 agaatttctg ggtattgttc atccttcacg ttttcatatt tttggtattt aattgaaaac
     9601 caagagtcta tactgccaac atcttcaaaa aatattggag ctgatgtttc cgcatgaaca
     9661 tatgcagaaa atgctaacaa aatggcaata cttagtttgg taaaaatatc gttccacact
     9721 tttcaggata atctattggg gggaagggat cttttcctct aaatctaatt gctcccactc
     9781 cttgtttgtc agaccattcg ccatttgcat cctgacgata ccagtgataa tcccctccca
     9841 tgcctataaa attgtgagga cgaataaaca attgagcttt atggtaacca gatggacaac
     9901 tcccattatc taaatccttg gcaccatcat tttttgacgc ttcaaaaata ctaccgcaag
     9961 tgagtttaga aaactctttt ccagataatt ctccaggttg taatcctccg ccgccaaaaa
    10021 tcctactccc agggtttcca tacctattta gagcatatga ataacaatta tttacggcaa
    10081 gataatacgg atctgaattt aggtctcgat tgaatactgg agcaggttta tcaattaagc
    10141 ccagcggatc ggtaatgact ccaacttact gatagtgttt tatgttcaga taatgcccga
    10201 tgaccttgtc atgcagctcc accgattttg agaacgacag tga
//
LOCUS       DAJTMC010000150        10213 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.150, whole genome shotgun sequence.
ACCESSION   DAJTMC010000150 DAJTMC010000000
VERSION     DAJTMC010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10213
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.150"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            141..1997
                     /gene="kup"
                     /locus_tag="OO130_003336"
     CDS             141..1997
                     /gene="kup"
                     /locus_tag="OO130_003336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HCR5366616.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSLTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            2164..2583
                     /gene="rbsD"
                     /locus_tag="OO130_003337"
     CDS             2164..2583
                     /gene="rbsD"
                     /locus_tag="OO130_003337"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HCR5366617.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            2591..4060
                     /gene="rbsA"
                     /locus_tag="OO130_003338"
                     /pseudo
     CDS             2591..4060
                     /gene="rbsA"
                     /locus_tag="OO130_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
     gene            4065..5030
                     /gene="rbsC"
                     /locus_tag="OO130_003339"
     CDS             4065..5030
                     /gene="rbsC"
                     /locus_tag="OO130_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HCR5366618.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGTIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            5055..5941
                     /gene="rbsB"
                     /locus_tag="OO130_003340"
                     /pseudo
     CDS             5055..5941
                     /gene="rbsB"
                     /locus_tag="OO130_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            6067..6996
                     /gene="rbsK"
                     /locus_tag="OO130_003341"
     CDS             6067..6996
                     /gene="rbsK"
                     /locus_tag="OO130_003341"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HCR5366619.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            7000..7992
                     /gene="rbsR"
                     /locus_tag="OO130_003342"
     CDS             7000..7992
                     /gene="rbsR"
                     /locus_tag="OO130_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HCR5366620.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(7958..9385)
                     /gene="mdtD"
                     /locus_tag="OO130_003343"
     CDS             complement(7958..9385)
                     /gene="mdtD"
                     /locus_tag="OO130_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HCR5366621.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMFIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     RATWHWIFLINIPIGIAGLLYARKHMPNFTTAQRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARHTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGSVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNRVPSESE"
     gene            complement(9408..10100)
                     /locus_tag="OO130_003344"
     CDS             complement(9408..10100)
                     /locus_tag="OO130_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HCR5366622.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKKNFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KTCQALLRSPDK"
BASE COUNT         2486 a         2458 c         2702 g         2567 t
ORIGIN      
        1 aaggggggta tttttctttg agccaggtta atgtggccgc atttaggagt acgattttgc
       61 cgttaatcgt gcatactgtg cgcttttttg tgggccaagg gactaagcac acatttcata
      121 tttcaacgaa agactagtct atgagcactg ataataagca atcattgccc gcgattaccc
      181 tcgcggcgat tggagttgtc tacggcgata ttggtaccag cccgttatat acacttcgtg
      241 aatgtttgtc cggccagttt ggttttggcg ttgaacgcga tgccgtgttt ggctttttat
      301 cgctgatctt ctggctgcta atctttgtgg tttccattaa atatctcacc ttcgtgatgc
      361 gggcagataa cgccggtgag ggggggatcc tgacgttgat gtcgcttgcc gggcgtaata
      421 cgtcggcgcg aaccacatca atgctggtga ttatggggct aatcggcggc agctttttct
      481 atggtgaagt cgtcataaca cccgctattt cggtgatgtc agccattgaa ggtctggaaa
      541 tcgtcgcccc gcagctggat acctggatag ttcccctctc aattatcgtt ctcactttat
      601 tatttatgat tcaaaaacat ggcacggcta tggtcggtaa gctgtttgcg ccgatcatgc
      661 tgacctggtt tttgattctg gcaggactgg ggttacgtag cattattgct aacccggaag
      721 tgctgcatgc gctgaatcca atgtgggcgg tgcatttctt ccttgaatac aaaacggttt
      781 cttttattgc attaggggca gtggtgttgt cgattacggg ggtcgaggcg ctgtatgctg
      841 atatggggca ctttggtaag ttccctattc gcctggcgtg gttcaccgtc gtattacctt
      901 ccttgaccct taattacttc ggtcagggag cgctgttgtt aaagaacccg gaagcgatta
      961 agaacccgtt cttcctgttg gcaccggact gggcgctgat cccgctgctg atcatcgccg
     1021 cactggcgac ggtaattgcc tcgcaggcgg ttatctctgg cgtcttctca ttgacgcgtc
     1081 aggcggtacg tctgggatat ttgtcgccga tgcgcattat tcacacctcc gaaatggagt
     1141 cagggcaaat ctatattccg tttgtgaact ggatgctcta tgtcgcggtc gtgattgtga
     1201 ttgtcagctt tgagcactcc agcaacctgg cggcggcgta cgggattgcg gtgaccggaa
     1261 ccatggtgct gacgtctatt ctctcgacta ccgtggcacg tcagaactgg cactggaata
     1321 agtattttgt tgcgctgatc ctgattgctt tcctttgtgt cgatattcca ttgttcaccg
     1381 ctaacctcga taaactgctc tccggcggtt ggttgccatt gagcctcggt acggtgatgt
     1441 ttatcgtgat gaccacctgg aagagcgagc gtttccgctt gctgcggcgg atgcatgaac
     1501 atggtaactc tctggaagcg atgattgctt cgctggagaa atcaccgcct gttcgcgtgc
     1561 ccgggaccgc ggtgtatatg tcgcgtgcaa tcaacgtcat tccctttgcg ctgatgcata
     1621 accttaaaca taacaaggta ttgcatgagc gggtgattct gttaactctg cgtaccgaag
     1681 acgcgccata tgtccataat gtccgtcggg tacagattga acaactgtcg ctcactttct
     1741 ggcgcgtggt ggcaagttat ggttggcgag aaacgccaaa cgtggaagaa gttttccacc
     1801 gctgcggtct ggaaggatta agttgccgga tgatggaaac ctccttcttt atgtcgcatg
     1861 agtcgaaacg cccgtggtat ttgcgtctgc gcggcaagct gtacttgctg ctgcaacgta
     1921 atgcgctgcg tgcaccagat caatttgaaa tcccgccaaa cagggttatc gaactgggta
     1981 ctcaggtcga aatctaacgc cagacgcctc ctttctccat aagggggcgt ttttgttttc
     2041 atggttaatc accatgtaaa acgtttcgag gttgatcaca tttccgtaac gtcacgatgg
     2101 ttttcccaac tcagtcagga ttaaactgcg ggtcagcgaa acgtttcgct gatggagaaa
     2161 aaaatgaaaa aaggcaccgt tcttaattct gatatttcat cggtgatctc ccgtctggga
     2221 cataccgata cgctggtggt gtgtgatgct ggtttaccca tccccaaaag tacaacgcgt
     2281 atcgatatgg cattaaccca gggtgtacct tcttttatgc aggtgctggg cgtcgttaca
     2341 aatgaaatgc aggtcgaggc tgccattatc gcggaagaga tcaaacaaca taatccgcaa
     2401 ctccacgaaa cgttgctcac tcaccttgag cagctgcaaa aacaccaggg aaataccatt
     2461 gaaattcgtt acaccacgca tgaacaattc aaacaacaaa ccgcagaaag tcaggcggta
     2521 attcgcagcg gagaatgttc tccgtatgcg aatatcattc tctgtgctgg cgtgacgttc
     2581 tgaggccgtc atggaagcat tacttcagct taaaggcatc gataaagcct tcccgggcgt
     2641 aaaagccctc tcgggcgctg cgttaaatgt ctatccgggc cgcgtgatgg cgctggtggg
     2701 cgaaaacggc gcgggtaaat ccaccatgat gaaagtgctt actggtatct atgctcgcga
     2761 tgccggcacg cttttatggc tggggaaaga aacgacattt accgggccga aatcttccca
     2821 ggaagccggg attgggatta tccatcagga actgaacctg atcccgcagt tgaccattgc
     2881 cgaaaacatt ttcctcggtc gtgagtttgt taatcgcttt ggcaaaattg actggaaaac
     2941 catgtatgcc gaagcggata aattgctggc taaacttaac ctgcgcttta aaagcgacaa
     3001 gctggtgggc gatctttcca tcggtgacca gcaaatggtt gaaatcgcca aagtgctgag
     3061 ctttgagtcg aaagtcatca ttatggatga accgaccgat gcgctgaccg ataccgaaac
     3121 cgaatccctg ttccgcgtca tccgcgaact aaaatcgcaa ggccgcggta ttgtctatat
     3181 ctcccaccgc atgaaagaaa tcttcgagat ttgcgatgcc gttaccgttt ttcgtgacgg
     3241 gcaatttatt gctgagcgcg aagtggcatc actgaccgaa gattcgctga ttgagatgat
     3301 ggtgggtcgc aagctggaag atcaatatcc acacctggac aaagcgccgg gagatatccg
     3361 cctgaaagtc gataatctct gcggacctgg cgttaacgat gtctctttta ctttacgcaa
     3421 aggcgaaatt cttggcgtct ctggtttgat gggcgcaggt cgtaccgaac tgatgaaagt
     3481 gctctacggc gcactaccgc gcaccagcgg ttacgtcacc ctggatgggc atgaagtcgt
     3541 tacccgttca ccgcaggatg gcctggcaaa cggcattgtg tatatctccg aagaccgtaa
     3601 acgtgacggt ttagtgttgg gcatgtcagt aaaagagaac atgtcgctga cagcgctgcg
     3661 ctacttcagc cgcgctggcg gcagtttgaa gcatgccgat gaacagtagg ctgtgagtga
     3721 tttcattcgt ctgtttaatg tgaaaacacc gtcgatggaa caggcaattg gtctgctttc
     3781 cggtggcaat cagcaaaaag tgttgatcct tgatgaacct acccgtggcg tagatgtcgg
     3841 cgcgaaaaaa gagatctatc aactgattaa ccagttcaaa gccgatggct tgagcatcat
     3901 tctggtgtca tcggagatgc cagaagtatt aggcatgagc gatcgcatca tcgtcatgca
     3961 tgaagggcat ctcagcgggg aatttactcg tgagcaggcc acccaggaag tgttaatggc
     4021 tgccgctgtg ggcaagctta atcgcgtgaa tcaggagtaa aaaaatgaca acccagactg
     4081 tctctggtcg ccgttatttc acgaaagcgt ggctgatgga gcagaaatcg cttatcgctc
     4141 tgctggtgct gatcgcgatt gtctcgacgt taagcccgaa ctttttcacc atcaataact
     4201 tattcaatat tctccagcaa acctcggtga acgccattat ggcggtcggg atgacgctgg
     4261 tgatcctgac ttcgggcatc gacttgtctg taggttctct gttggcgcta accggcgcag
     4321 ttgctgcatc tatcgtcggc attgaagtca atgcgctggt ggctgtcgct gctgctctcg
     4381 cgttaggtgc cgcaattggt gcggtaaccg gggtgattgt agcgaaaggt cgcgttcagg
     4441 cgtttatcgc tacgctggtt atgatgcttt tactgcgcgg cgtgaccatg gtttatacca
     4501 acggtagccc agtgaatacc ggctttactg agaacgccga tctgtttggc tggtttggta
     4561 ttggtcgtcc gctgggcgta ccgacgccag tctggatcat ggggattgtc ttcctcgcgg
     4621 cctggtacat gctgcatcac acgcgtctgg ggcgttacat ctacgcgctg ggcggcaacg
     4681 aagcggcaac gcgtctttct ggtatcaacg tcaataaaat caaaatcatc gtctattctc
     4741 tttgtggtct gctggcatcg ctggccggga ccattgaagt ggcgcgtctc tcctccgcac
     4801 aacccacggc ggggactggc tatgagctgg atgctattgc tgcggtggtt ctgggcggta
     4861 cgagtctggc gggcggaaaa ggtcgcattg ttgggacgtt gatcggcgca ttaattcttg
     4921 gcttccttaa taatggattg aatttgttag gtgtttcctc ctattaccag atgatcgtca
     4981 aagcggtggt gattttgctg gcggtgctgg tagacaacaa aaagcagtaa taacgactac
     5041 aggacatctt gaatatgaac atgaaaaaac tggctaccct ggtttccgct gttgcgctaa
     5101 gcgccaccgt cagtgcgaat gcgatggcaa aagacaccat cgcgctggtg gtctccacgc
     5161 ttaacaaccc gttctttgta tcgctgaaag atggcgcgca gaaagaggcg gataagcttg
     5221 gctataacct ggtggtgctg gactcccaga acaacccggc gaaagagctg gcgaacgtgc
     5281 aggacttaac cgttcgcggc accaaaattc tgctgattaa cccgaccgac tccgacgcag
     5341 taggtaatgc tgtgaagatg gctaaccagg cgaacatccc ggttatcact cttgaccgcc
     5401 aggcaacgaa aggtgaagtg gtgagccaca ttgcttctga taacgtactg ggtggcaaaa
     5461 tcgccggtga ttacatcgcg aagaaagcgg gtgaaggtgc caaagttatc gagctgcaag
     5521 gcattgctgg tacatccgca gcccgtgaac gtggcgaagg cttccagcag gccgttgctg
     5581 ctcacaagtt taatgttctt gccagccagc agattttgat cgcactaaag gtttgaacgt
     5641 aatgcagaac ctgttgaccg ctcatccgga tgttcaggct gtattcgcgc agaatgatga
     5701 aatggcgctg ggcgcgctgc gcgcactgca aactgccggt aaatcggatg tgatggtcgt
     5761 cggatttgac ggtacaccgg atggcgaaaa agcggtgaat gatggcaaac tagcagcgac
     5821 tatcgctcag ctacccgatc agattggcgc gaaaggcgtc gaaaccgcag ataaagtgct
     5881 gaaaggcgag aaagttcagg ctaagtatcc ggttgatctg aaactggttg ttaagcagta
     5941 gttttaatca ggttgtatga cctgatggtg acataaatac gtcatcgaca gatgaacgtg
     6001 taatataaag aaaagcaggg cacgcgccac cctaacacgg tggcgcattt tatggacatc
     6061 ccgaatatgc aaaacgcagg cagcctcgtt gttcttggca gcattaatgc tgaccacatt
     6121 cttaatcttc aatcttttcc tactccaggc gaaaccgtaa ccggtaacca ctatcaggtt
     6181 gcatttggcg gcaaaggcgc gaatcaggct gtggctgctg ggcgtagcgg tgcgaatatc
     6241 gcgtttattg cctgtacggg tgatgacagc attggtgaga gcgttcgcca gcagctcgcc
     6301 actgataaca ttgatattac tccggtcagc gtgatcaaag gcgaatcaac aggtgtggcg
     6361 ctgatttttg ttaatggcga aggtgagaat gtcatcggta ttcatgccgg cgctaatgct
     6421 gccctttccc cggcgctggt ggaagcgcaa cgtgagcgta ttgccaacgc gtcagcatta
     6481 ttaatgcagc tggaatcacc actcgaaagt gtgatggcag cggcgaaaat cgcccatcaa
     6541 aataagacta tcgttgcgct taacccggct ccggctcgcg aacttcctga cgaactgctg
     6601 gcgctggtgg acattattac gccaaacgaa acggaagcag aaaagctcac cggtattcgt
     6661 gttgaaaatg atgaagatgc agcgaaggcg gcgcaggtac tgcatgaaaa aggtatccgt
     6721 actgtactga ttactttagg aagtcgtggt gtatgggcta gcgtgaatgg tgaaggtcag
     6781 cgcgttcctg gattccgggt gcaggctgtc gataccattg ctgccggaga tacctttaac
     6841 ggcgcgttaa tcacggcgtt gctggaagaa aaaccattgc cagaggcgat tcggtttgcc
     6901 catgctgcag ctgcgattgc cgtaacacgt aaaggcgcac aaccttccgt accgtggcgt
     6961 gaagagatcg acgcattttt agacaggcag aggtgacgct tggctacaat gaaagatgtt
     7021 gcccgcctgg cgggcgtttc tacctcaaca gtttctcacg ttatcaataa agatcgcttc
     7081 gtcagtgaag cgattaccgc caaagttgaa gcggcgatta aagaactcaa ttacgcgcca
     7141 tcagccctgg cgcgtagcct caaactcaat caaacacata ccattggcat gttgatcact
     7201 gccagtacca atcctttcta ttcagaactg gtgcgtggcg ttgaacgcag ctgcttcgaa
     7261 cgcggttata gtctcgtcct ttgcaatacc gaaggcgatg aacagcggat gaatcgcaat
     7321 ctggaaacgc tgatgcaaaa acgcgttgat ggcttgctgt tactgtgcac cgaaacgcat
     7381 caaccttcgc gtgaaatcat gcaacgttat ccgacagtgc ctactgtgat gatggactgg
     7441 gctccgttcg atggcgacag cgatcttatt caggataact cgttgctggg cggagactta
     7501 gcaacgcaat atctgatcga taaaggtcat acccgtatcg cctgtattac cggtccgctg
     7561 gataaaactc cggcgcgcct gcggttggaa ggttatcggg cggcgatgaa acgtgcgggt
     7621 ctcaacattc ctgatggcta tgaagtcact ggtgattttg aatttaacgg cgggtttgac
     7681 gccatgcgcc aactgttatc acatccgctg cgtcctcagg ccgtctttac cggaaatgac
     7741 gctatggctg ttggcgttta ccaggcgtta tatcaggcag agttacaggt tccgcaggat
     7801 atcgcggtga ttggctatga cgatatcgaa ctggcaagct ttatgacgcc accattaacc
     7861 actatccacc aaccgaaaga tgaactgggg gagctggcga ttgatgtact catccatcgg
     7921 ataacccagc cgacccttca gcaacaacga ttacaactta ctccgattct gatggaacgc
     7981 ggttcggctt agatttacgc tgtcttttga tcaaattatt accatcggtt gttttcagaa
     8041 gcatgaacat tgctgctgaa gcaacagtaa taatgcccat cgtgataaac gtatagtgga
     8101 attgttcgac agtcgttgtg ccttccatcc cttcataaac gcgaaggacg gccgcactta
     8161 cagcaacgcc taaactaatc gacagttgct gcgtgaccgc cagaacactg ttaccgctgc
     8221 tggcgttgtc atcggtcaga tcggcaagtg tgatggtatt catcgcggta aattgcgtcg
     8281 acatagccat ccctaatata aacaacggca agatcagcat ccatatcgcc attgccggtg
     8341 attgcaaaga gaactgagcg atcattagcc caataatcac cgtgatcccc actaacgtat
     8401 ggcgatagcc cagacgacgt aagacttggg taaccatcga ttttgcaata atggaaccta
     8461 acgctgtcgg tgccatcata cagccggcaa taaacgcctg ataaccaaat cctacctgta
     8521 acatcaatgg cataaggaac ggtacactac cggtccccag acgggttgca atattgccta
     8581 cgataccgat cgagaaagtg cgggttttaa ataaatctaa tgaaattaat gggtttggcg
     8641 tgtgtcgcgc atggagaata tagagaagca gtaacccgat gctggtgaca attaccgtca
     8701 aggcaatcca gctggcgaca atcttttccc cgaatagctc tattccgctt gagaagagaa
     8761 caaggctgag gccaaacagc aaaaagccag tgatatcgaa tctgcgttgt gcggtggtga
     8821 aattgggcat atgtttgcgc gcgtaaagaa ggcccgcaat acctatgggg atattgatta
     8881 aaaatatcca gtgccaggtt gccctggtga ccagcacgcc gccaagaacg gggcctaaaa
     8941 ttggccccac cagacccggc atggcgacaa aattcaatac aggaagaagt tcattacgag
     9001 gataagcacg cagtaaggcc agccgagcaa caggcatcat cattgcgccg cctatcccct
     9061 gaataacccg gaagacaacc agctgtggta gcgaattaga aagtgcgcag gccagagaac
     9121 ccaacgtgaa cagactcacg gcaagggtaa aaatgcgacg cgtaccgaag cgatcggcta
     9181 gccatccgct taccggaata aacatcgcca ccgtcagcgt ataactgatg atggctgatt
     9241 gcatcgcgag aggagaacga ttaaggctat gagcgattgc gggtaaggcg gtattcagaa
     9301 tagtggcatc aagtgcctgc atgaagaagg ccatcgccgc gatccacggc aaacccgcca
     9361 tactacgctt ctttttatcg ctcattcaat gtcctgttat cgggttatca cttatcaggt
     9421 gagcgtagca gcgcctgaca agttttaaat gccgcgtcgc catcgctttg gataatcgca
     9481 tcgacaatcg cctggtgcag atccagcttt atcactgtgt cgctggtaat tgacgtgaag
     9541 taagtgtgat aaaccgaatg gaatagcgag gcgaatgagg tcaaaaacgg attggcgctc
     9601 atttcataga tatgctcatg ccaggccata tcgacttcga tccagcgttc acggcgaaag
     9661 ttctttttta atgccgccat ttcggccatt aacgtattga gatgcgcctt ctgttccgcg
     9721 gtgccaaccg ttgctgccag taggcaggct tgcggctcca gacagatacg cataaccaga
     9781 aagtgatcga tgacctgatg aaagttctct tctgtcatcc accaggtaag caattcctga
     9841 tcaagaaaat tccagtttga ttgtggcatg acccgggtac caattcgcgg tcgcggtaaa
     9901 accatccctt ttgccgttaa cgttttgacc gcttcgcgta ccgctgtacg actcactcca
     9961 aattgctcgc ccagctcaat ctcaccaggc aaaatggtgc cgggttcata ttcacctttt
    10021 aagatgcgtt gcgccagctt ctcagccaga acatacgaaa ggtttttctg tgcagctaac
    10081 tgttgtgcgc ttaaaggcat tactaatctt cctttttctt tttattcctc cttagtatgc
    10141 caccaggaag tgtgattacg gttgcaaaaa cggcaaattg cttgttttat ggcacattaa
    10201 cggggctttt gct
//
LOCUS       DAJTMC010000151        10118 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.151, whole genome shotgun sequence.
ACCESSION   DAJTMC010000151 DAJTMC010000000
VERSION     DAJTMC010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10118
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.151"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            203..355
                     /gene="hokA"
                     /locus_tag="OO130_003345"
     CDS             203..355
                     /gene="hokA"
                     /locus_tag="OO130_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HCR5366623.1"
                     /translation="MPQKYGLLSLIVICFTLLFFTWMVRDSLCELHIKQGRYELAAFL
                     ACNLKE"
     gene            complement(543..755)
                     /gene="cspA"
                     /locus_tag="OO130_003346"
     CDS             complement(543..755)
                     /gene="cspA"
                     /locus_tag="OO130_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HCR5366624.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(1036..1326)
                     /locus_tag="OO130_003347"
     CDS             complement(1036..1326)
                     /locus_tag="OO130_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HCR5366625.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGISVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            1759..2469
                     /locus_tag="OO130_003348"
     CDS             1759..2469
                     /locus_tag="OO130_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HCR5366626.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQTDDLKPVFDQAFTKVVTTP
                     ADALQPLIPVAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2519..3493)
                     /gene="ghrB"
                     /locus_tag="OO130_003349"
     CDS             complement(2519..3493)
                     /gene="ghrB"
                     /locus_tag="OO130_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HCR5366627.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKPSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3597..4256)
                     /locus_tag="OO130_003350"
     CDS             complement(3597..4256)
                     /locus_tag="OO130_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HCR5366628.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLDSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            4409..6742
                     /gene="bisC"
                     /locus_tag="OO130_003351"
     CDS             4409..6742
                     /gene="bisC"
                     /locus_tag="OO130_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HCR5366629.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVHVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKTAEWAAGICGVHAAKIRELAALFHQNTTMLMAGWGMQRQQLGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRCSAVLSSMQGSLPGGSDAVDK
                     IPVARIVEALENPGCAYQHNGMNRLFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSELQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFCRDPQAHPLKTASGKIEIFSQRIADYAYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDALARGIQD
                     GDTVRLWNARGQILAGVVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(6711..7151)
                     /locus_tag="OO130_003352"
     CDS             complement(6711..7151)
                     /locus_tag="OO130_003352"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR5366630.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDSKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRHPHLML
                     EVYQKNQPAINFYQAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(7148..7711)
                     /gene="tag"
                     /locus_tag="OO130_003353"
     CDS             complement(7148..7711)
                     /gene="tag"
                     /locus_tag="OO130_003353"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HCR5366631.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTPASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            7869..8567
                     /locus_tag="OO130_003354"
     CDS             7869..8567
                     /locus_tag="OO130_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR5366632.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            8795..9997
                     /locus_tag="OO130_003355"
     CDS             8795..9997
                     /locus_tag="OO130_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5366633.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTMASGILLGLGFFLTAHSDNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHI"
BASE COUNT         2427 a         2435 c         2592 g         2664 t
ORIGIN      
        1 ttttaggatt atatcaagga gaagaaacaa acttattaag ctagaatagc tacgggtgct
       61 tgagactgtt tgtctcaggt attcaccgaa aggcagacag agaaaagccc cacctgacta
      121 taaatcaaag tgaggctacc ctatgcctga acaccataag gttagcctct tactcgttgg
      181 aaatcaacac agggggctgg gaatgccgca aaaatacgga ttactttcat taatagtgat
      241 ttgtttcacg cttttatttt tcacctggat ggtaagagat tcactgtgtg aattgcatat
      301 taaacagggg cgttatgagc tggcggcatt tttagcctgc aatttaaaag agtaagagtc
      361 ttcggcggga aattattccc gcctttctta cggcgttgcg cattctcatt gcacccacat
      421 ttattcttca caaaaataat aatagatttt actacgcgat cgattattta tttcctgaaa
      481 aaaaataaaa aaaatccccg ccaaatggca gggatcttag attctgtgct tttaagcaga
      541 gattacaggc tggttacgtt accagctgcc gggcctttag cgccgctttc gatggtgaag
      601 gacactttct gaccttcgtc cagagatttg taaccatcgt tctggatagc agagaagtgt
      661 acgaacacat ctttagagcc atcgtcagga gtgatgaagc cgaagccttt gtcagcgttg
      721 aaccatttta cgataccagt cattttaccg gacatagtgt attaccttta ataattaagt
      781 gtgcctttcg gcgatatggc gtgctttaca gattttgaag cgttaaagga atgtgcacta
      841 cgaggggtat caacgataac tcttgaaggg acttgcctta ctacactgct ttaatggtct
      901 gtacgtcaaa ccgttgatgt gcattaagcc acgcattggc gggtgatgca acaattattt
      961 ttcatattta tgattaatcg gttggaaaac ggtcctgtca tcaggaccgt aaacagcaat
     1021 aaagtggata aaagtctatt ccatcaactg cttacttaat gccgggttgg cttgaatcaa
     1081 acgcatcaat tttagttcgg cacttgaagg cttcacgcgt ctggattccc attcctttac
     1141 catggcgact gatatgccca aaacccgggc gaaatcatcg atttttaacc ctgtcccttt
     1201 tcgtaactgc tcaatttcgg tacaggacgt tgttctgtgc gtcagggtaa ttttctgcgt
     1261 ttcatcttta aaaacaatct gttccaggct gctcaacagc tcatgcattg gatctttata
     1321 ttccattgaa aactcctcaa atcacactgc gggatcgtga attgcatcga agctcattaa
     1381 gaatagtcgg gaaaagcaga cacagcgggg tgaagtcagg gcatgctctg gctatgattc
     1441 gattcagcca gtcatttagc gtgacttata gccacctaac gatctgataa atgccgatat
     1501 atctcttacc actcctgttt tgtaaagatt tcgaaaatct aaagttctgc gaaggcgtaa
     1561 atctcgggct acaaaaaagc aacggcaata tcatttttta acaatgaaac aacaaattaa
     1621 ccttgtttca ttttctccat gattttacca ggaagattac gtagaaaggg tatcctgcaa
     1681 gcctgtcctg gactatcctt gtcacgtcag acacgcgtgt gtcgttgtgc gctttttttg
     1741 ggtgaaagga gtaagaaaat ggcgacagga aagtcctgct ctcgctggtt tgcgcctctt
     1801 gcggcgttat taatggtagt tagcctgagt gggtgttttg ataaagaagg cgatcagcgt
     1861 aaagcgttta tcgacttcct gcagaataca gtgatgcgta gcggtgaacg tctaccaacc
     1921 ctgactgccg atcagaaaaa acagtttggt ccttttgtct ctgattacgc gattctgtat
     1981 ggttattctc agcaggtgaa tcaggcgatg gattccggtc tgcgcccggt cgtagacagc
     2041 gttaacgcaa ttcgcgtacc gcaggattac gttacgcaaa gcggtccgct gcgtgaaatg
     2101 aacggttcgc tgggtgtgct ggcgcaacaa ctgcaaaatg cgaagttgca ggccgatgct
     2161 gcgcactctg cgctaaaaca gaccgatgat ttgaaaccgg tctttgatca ggcattcacc
     2221 aaagtggtaa cgacgccagc tgatgcattg caaccgttga ttccggttgc acaaactttc
     2281 acgcaacaac tggtgatggt tggagactac attgctcaac aaggtactca ggtgagcttt
     2341 gtagcaaatg gtatccagtt cccgacttcg cagcaggcaa gtgaatataa caaactgatt
     2401 gcgccgttac cggcacaaca tcaggcgttt aatcaggcct ggactacagc agttactgca
     2461 acccaataaa gagtaaaagc ccgagcgatt atcgggcttt acttcacgca gtcgcggctt
     2521 agtccgcgac gtgcggattc acacagttct tctcaacctt tccttgtaac gcatcaatca
     2581 aattatccac ggcacaggcg gccatgccat aacgcgtctc atgggtggca gatccaatat
     2641 gcggtactgc gacgacgttg gccattgaga gcaacggcga atctacggac agtggctctt
     2701 gttcgaagac atccagcccg gcggcgtgaa tttcgccttt ctgcaatgct gcgatcagtg
     2761 cattttcgtc aaccaccggg ccacgtccgg cattaatgaa aatggcggag ggtttcattt
     2821 tggcgaattg ttctgcgcca aacagatgat gcgtctcatc agttaacggc aggatcaggc
     2881 aaacgaaatc tgactcttgt aacagagtat ccaaatcgca gtagcgggcg ttgaagcgtt
     2941 cttctgcttc tttatggtgg cggcgcgcgt tatagaggat gggcatgttg aagccaaagt
     3001 gcgcacgttg tgccagcgcc atgccgatcc gtcccatccc gacaatgccc agtgttttat
     3061 ggtgaacgtc agtgccgtac cagtccgggc ctatgctcgc ggtccattcg cctgctttta
     3121 cccgttctgc tacctccaca acccgacgag cggtagacaa caccagcgcc atcagcgtat
     3181 cggcgacggt ttctgttaat acggttggcg tgtgcatcag cagaattttt cgggcggtaa
     3241 gcgcatcgac atcaaaattg tcatagccga cggagatcgt tgatgtggca cgcagtttcg
     3301 gcattttttc cagcaatgcg gcatctacat tctcgtttga acccagtaaa ccttcagctt
     3361 cggcaaaaat tgctgcattt tgttcgacgg tttgtgggct gaggtttgcc acctggtgaa
     3421 cggtgaaatg ctcttgcagg cgttgcagta aatcatcagg taaggctttg tagaggataa
     3481 cggacggctt catgcttctc tccattcact gataaggcgc tcaccttagc ctgaaatatg
     3541 atgaaggaaa actgaagaat gccaggtgat ttctgcatca cctggcatga aagggattac
     3601 agcgggctta aggtaatttc tacacggcgg ttttgcgcct taccttctgc ggtgctgttg
     3661 ctggcgattg ggttagccgg gccaaggccc tgagtacgga tgcggctggc gtccacgccc
     3721 tgggtgatca acgcgctggc aacggaatcc gcacgctgct gggagagacg catgttcagg
     3781 tcgtgacccc ccgtgctgtc ggtataacca atcacgttaa ccgccgtttt cggatactct
     3841 ttcagcacca ttgccacgcc ggtcagggtg ttagcgcctg ccggtttcag ggtcgcgctg
     3901 ctgctgtcga aggtcacatt gttcggcata ttgaggataa tgttatcccc gctgcgggtt
     3961 acgctaacac cagtgccgcg cattttgtca cgcagcttcg cttcctgcac atccatgtaa
     4021 taacccacgc cgccgcccag agctgcgcct gctgctgcgc caatcagcgc gcctttaccg
     4081 cgatctttct tcgaagaaga gagcgcacca atacccgcgc ccacgagaga gtccagacct
     4141 gcgccgatag cagatttgcc tgcttcgcgt tcgccggtgt aagggttagt tgtgcagcca
     4201 gataccgcca gagcgccact cactacggcg gcaataagat aaacacgttt cttcattgtt
     4261 aatccttaat aaccttttta ttctttgcca cgggttccgt ggcgggagat tatgccgcgt
     4321 gaacatgaag attattcctg ggaatactcg gaaatttgta agtaatattt aactgctcaa
     4381 tacatctaac ctttcaggag tcttcggttt ggccaactca tcctcacgat attccgttct
     4441 gactgccgcc cactgggggc ccatgctggt tgaaaccgac ggcgaaaccg tgtttagctc
     4501 gcgtggcgcg ttagccacag gaatggaaaa ctccttgcag agcgcggttc gcgaccaggt
     4561 tcacagcaat acgcgggtac gatttccaat ggtgcgcaaa ggctttctcg cgtcaccgga
     4621 aaacccgcaa ggcattcgtg ggcaggatga atttgttcac gtgagttggg atgaggcgct
     4681 ggatcttatt caccaacaac ataaacgcat tcgtgaggct tatggtccgg catcgatttt
     4741 tgctggttcc tacggctggc gttcaaacgg cgtgctgcat aaggcctcga cattattaca
     4801 acgctatatg gcgctggcag gcggttatac cgggcatctg ggggattatt cgaccggtgc
     4861 ggcacaggcg atcatgccgt atgtcgtggg tggcagcgaa gtttatcaac agcaaaccag
     4921 ttggccgctg gtgctggaac atagcgatgt cgtggtgttg tggagtgcta acccactcaa
     4981 tacgctgaaa attgcgtgga atgcatctga tgagcagggg ctttcttact tttccgcact
     5041 gcgtgacagc gggaaaaagc tgatctgtat tgatccaatg cgatcggaaa ccgtcgattt
     5101 ctttggcgat aaaatggagt gggtggcacc gcacatgggc accgatgttg cgctgatgct
     5161 ggggatcgcc catacgctgg tggaaaatgg ttggcacgac gaagcgtttc tggcgcgttg
     5221 caccacaggt tatgccgtct tcgcctctta tttgctgggc gagagtgacg gaatagccaa
     5281 aaccgccgaa tgggcggcag ggatttgtgg ggttcacgca gcgaaaattc gcgagttggc
     5341 ggctcttttc caccaaaaca cgaccatgct gatggctggc tggggaatgc agcgccaaca
     5401 gcttggtgag caaaaacact ggatgatcgt cacgctggcg gcaatgttgg ggcaaatcgg
     5461 cacacccggc ggcggttttg gtctttctta tcattttgcc aatggtggta atcccacgcg
     5521 gtgttctgcg gtgctctctt ccatgcaggg tagcttgccg ggtggcagcg atgcggtaga
     5581 taaaatccct gttgcccgta ttgttgaagc actggaaaac cctggctgcg cataccaaca
     5641 caatggcatg aatcgcctct tcccggatat tcgctttatc tggtgggcgg gtggcgccaa
     5701 cttcacccat catcaggaca ctaatcgcct gatacgagct tggcaaaaac cggagctggt
     5761 agttatctcc gaatgcttct ggacggcggc ggcaaaacac gcggatatcg ttctgcctgc
     5821 gactacctct tttgagcgta atgatctcac catgaccggc gattacagta atcagcatct
     5881 ggtgccgatg aagcaagtgg tgccgccacg ctatgaagcg cgtaatgatt ttgatgtttt
     5941 tgccgagtta agtgaacgct gggagaaggg cggttatgca cgttttacgg aaggaaaaag
     6001 tgagttgcaa tggctggaaa cgttttataa cgttgcccga cagcgcgggg caagccagca
     6061 ggttgaattg ccgccatttg ctgagttctg gcaagccaat cagttaattg agatgccgga
     6121 aaacccggac agcgagcggt ttattcgctt cgctgatttt tgccgcgatc cgcaggcgca
     6181 tccattaaaa accgccagcg gtaagattga aatcttctcg cagcgtattg ccgattacgc
     6241 ttatccggat tgcccggggc atccaatgtg gctggagccg gacgaatggc agggcaatgc
     6301 cgaaccggaa cagttacagg tactttctgc tcatccggcg catcgtctgc acagccagct
     6361 gaattacagt tctctgcgcg aattgtatgc ggtggcaaat cgtgagcctg tcaccattca
     6421 tcctgacgat gccctggcgc gcggcattca agatggcgat actgttcggc tgtggaacgc
     6481 acgcgggcaa attcttgccg gagtggtcat tagcgaggga attaaacctg gcgtgatttg
     6541 tattcacgaa ggggcatggc cggatttgga tttaaccgct gacggtattt gtaaaaacgg
     6601 cgcagtcaac gtgctgacca aagatctccc cagctcgcgg ctggggaatg gctgtgcggg
     6661 taatacggcg ctggcatggc tggaaaaata caacggtccg gaactgacac ttacagcgtt
     6721 tgaaccaccg gccagctcat aatccaggtg ggtagttggg tttcatcctg ccatgcgcaa
     6781 tcgacaatgt gaaaaccctg cgcctggtaa aaatttatcg ccggttgatt tttttgataa
     6841 acctccagca tcaggtgggg atggcgctgc tgcacatatt gcatcagcgc cttaccaata
     6901 ccgcgcctga cggccttcgg tgcgacaaac atcgctgcca gaaatcggcc ttccataatg
     6961 ctgacaaaac cgagaagctt actgtcttct tcccagaccc agttttgcgc attggcaaga
     7021 tacgcatccc gcaccagcgg aatgcaatca cgccagtaat tcgcttttat aaagggatgt
     7081 ccccaggttg tactttccag ccacagttcg aggatcgcgg ggagttctga acgttgcgct
     7141 tcccgaatca tggtttattt cccggatagc aacagcagcc aaccacatga tcattcacca
     7201 gcccacatgc ctgcataaag gagtaacaga ttgtggtacc gacaaactta aaaccacgtt
     7261 ttttcagtgc tttagatagg gcgtcggagg cgggcgtaga tgtggggatt tcgctcaacg
     7321 ttgtggcttg tgtcacctgt ggctgatgat ttacaaacga ccagacaaag tcggcaaacg
     7381 gttcgccgtt ctgttccatt tgcaggtacg cccgcgcatt accaataatt gcctgaattt
     7441 tcccgcgatg gcggataatc ccggcgtcct gtaccagtct ttcgacatcc tcttcctgca
     7501 ttgctgcgac cttcaccgga tcgaactgat gaaagcaggc gcgatagttt tcgcgttttt
     7561 tgaggacggt gatccacgat aacccagcct gctgcccttc aaggcagatc atttcgaaca
     7621 gttttttact gtcagtttca ggcacgcccc actcattatc atggtaggca atataaagcg
     7681 ggtcctgact cacccagccg caacgttcca tactttccct cgctatgtac ccgatcgtaa
     7741 aaatttcagc cgcttttctt gacgcatcgg tgaaaaagtc aatatttaat acagggctac
     7801 tatgcccgat atccttcata caatgacaat aatattgccg aattcggctc ttccctggag
     7861 tcgtgttaat gatcataaaa aaaagcggtg gtcgctggca gctaagcctg ctggcgagcg
     7921 tggtaatcag cgcctttttt ctcaacacag cttacgcctg gcaacaagaa tatatcgttg
     7981 atacgcaacc cggacattcc acagagcgtt acacctggga tagtgatcat caacctgatt
     8041 acaacgatat tttgtcgcaa cgtattcaaa gtagccaaag ggcgctggga ctggaagtca
     8101 atctggcgga agaaactcct gtggatgtga ccagcagtat gagtatgggc tggaattttc
     8161 ctttatatga acaggttaca accggcccgg tcgcggcatt acattacgat ggcacaacca
     8221 cctcgatgta taacgagttt ggcgacagta ctaccacgct gaccgatccg ttatggcatg
     8281 ccagcgtgag tagcttaggc tggcgtgttg actcccggct tggcgatctc cgaccctggg
     8341 cgcaaatcag ctataaccag caatttggcg agaatatctg gaaggcgcaa tcaggcctga
     8401 gccggatgac ggcgacaaac cagaacggca actggctgga tgtcaccgta ggcgctgata
     8461 tgttgctcaa tcaaaatatt gccgcctatg ccgcgctaac tcaggcagaa aataccacta
     8521 ataatagcga ctatctgtat acgatggggg ttagcgccag attttaacgt aacagtcaca
     8581 attgaaacca ttgaataaca atagttgtgg tgatagtggg tgctaactta caaataataa
     8641 atttggtgaa taattgtcgc gtcattcatt cctgaactaa ggcatttcat tccgttctga
     8701 tggcatttca tgccgttttt ccccaggcat aaaatgcact tcgttatggt tgtcggcaga
     8761 gatttttcct ttttattact gcaggaatac tgccatgaca ccttcaaatt atcagcgtac
     8821 ccgctggctg acactcatcg gtactatcat tacccagttt gcgctggggt cggtttatac
     8881 ctggagcctg tttaatggcg cgctttctgc caagctggat gcgccggtaa gccaggtcgc
     8941 tttctctttc ggcttgttaa gtctggggct ggcaatttca tcttctgttg cggggaaatt
     9001 acaggaacgt tttggcgtta aacgcgtcac tatggcttcc ggcattttgc taggattagg
     9061 cttcttcctg acagcgcatt ctgacaacct gatgatgctg tggttaagcg ccggtgtgct
     9121 ggtcggtctg gcggatggcg cgggttacct gctgacgctc tctaactgcg tgaagtggtt
     9181 cccggagcgt aaaggtctga tctccgcgtt cgctatcggt tcttatggtc ttggtagcct
     9241 gggtttcaaa tttatcgaca cgcagctgct ggaaacggtg ggtctggaaa aaacatttgt
     9301 gatttgggga gcgattgcgc tggtgatgat tgttttcggc gcaacgttaa tgaaagacgc
     9361 accaaaacag gaagtgaaaa ccagcaatgg tgtggtggag aaagattaca cgctggcaga
     9421 gtcgatgcgt aaaccgcagt actggatgtt agcggtaatg ttcctgactg cctgcatgag
     9481 cggcctgtac gtgattgggg tagcgaaaga tatcgcccaa agtctggcac accttgatgt
     9541 ggtttccgca gccaatgcag taacggttat ttccatcgct aacctttcag gtcgtctggt
     9601 gctgggcatt ctgtctgaca aaattgcccg tatccgtgtt atcaccattg gtcaggtgat
     9661 ttcgctggtg ggtatggcgg ccctgctgtt tgcaccatta aatgcagtga cgttctttgc
     9721 agcgattgcc tgcgtggcat ttaactttgg cggcactatt accgtctttc catcactggt
     9781 cagtgagttc ttcggcctca ataacctggc gaaaaactac ggtgtgattt acctcggttt
     9841 cggtatcggt agcatttttg gttcgattat cgcctcactg tttggcggtt tctatgtgac
     9901 cttctacgtg attttcgccc tgctgattct gtcattggcg ctttctacga cgattcgtca
     9961 gccagagcag aaaatgttgc gtgaggcgca tatttagaaa tatgttgcta ctgaaataaa
    10021 tacgaggagg gagacttcaa agtctccctt ttttatggac gcatattaaa agaacaataa
    10081 tattcatacc acaaatatat tttatttata tattatgt
//
LOCUS       DAJTMC010000152        10058 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.152, whole genome shotgun sequence.
ACCESSION   DAJTMC010000152 DAJTMC010000000
VERSION     DAJTMC010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10058
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.152"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            <1..206
                     /locus_tag="OO130_003356"
     CDS             <1..206
                     /locus_tag="OO130_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5366634.1"
                     /translation="STLKSPSYTKSVSWQHYRHICCYRRKLTDAELEACYRYDKQFWE
                     HYSKKCRWPIRNVPWGASSVKYS"
     gene            complement(323..1666)
                     /gene="gdhA"
                     /locus_tag="OO130_003357"
     CDS             complement(323..1666)
                     /gene="gdhA"
                     /locus_tag="OO130_003357"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HCR5366635.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQVQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSESEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVGAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            1902..2174
                     /locus_tag="OO130_003358"
     CDS             1902..2174
                     /locus_tag="OO130_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HCR5366636.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLLWRRVKP"
     gene            complement(2140..2547)
                     /gene="nudG"
                     /locus_tag="OO130_003359"
     CDS             complement(2140..2547)
                     /gene="nudG"
                     /locus_tag="OO130_003359"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HCR5366637.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVELDES
                     QQQALVRELNEELGIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPTDIPLLEAFMALRAARAAD"
     gene            2634..3245
                     /locus_tag="OO130_003360"
     CDS             2634..3245
                     /locus_tag="OO130_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCR5366638.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRELTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFISTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWIFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            complement(3254..4561)
                     /gene="ynjE"
                     /locus_tag="OO130_003361"
     CDS             complement(3254..4561)
                     /gene="ynjE"
                     /locus_tag="OO130_003361"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HCR5366639.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKADAPVALY
                     GNDKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAGARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPEQQVSFYCGTGWRAS
                     ETFMYARAMGWKNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(4628..5281)
                     /locus_tag="OO130_003362"
     CDS             complement(4628..5281)
                     /locus_tag="OO130_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR5366640.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     STLKGTARRNAVKDALDRAGLAETYHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRELAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            complement(5281..6816)
                     /locus_tag="OO130_003363"
     CDS             complement(5281..6816)
                     /locus_tag="OO130_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5366641.1"
                     /translation="MVTPLRYALIFLLWAMVAVIYAPLIPAALTLISPALSLTHWQAL
                     FADPQLPHALLATLVSTTIAAVGALLIALLVIVALWPGPKWQRMCARLPWLLAIPHVA
                     FATSALLLFADGGLLYDYFPYFTPPMDRFGIGLGLTLAVKESAFLLWILAAVLSEKRL
                     LQQVIVLDSLGYSRWQCLNWLLLPSVAPSLAMAMLAIVAWSLSVVDVAIILGPGNPPT
                     LAVISWQWLTQGDADQQTKGALASLLLMLLLAAYVLLSYLLWRSWRRTIPRVDGIRKP
                     ATPLLPGTTLASFLPLTGVLCVVLLAILADQSTINSEALINSLTMGLVATFIALLLLL
                     LWLEWGPQRRQLWLWLPILLPALPLVAGQYTLALWLNLDGSWTAVVWGHLLWVMPWML
                     FILQPAWQRIDSRLILIAQTLGWSRAKIFFYVKCTLMLRPVLIAFAVGFSVSIAQYMP
                     TLWLGAGRFPTLTTEAVALSSGGSNGILAAQALWQLLLPLIIFALTALAAKWVGYVRQ
                     GLR"
     gene            complement(6789..7955)
                     /locus_tag="OO130_003364"
     CDS             complement(6789..7955)
                     /locus_tag="OO130_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR5366642.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTGWAETLPNWRYVDTQLPVREDFSVPTQGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPAALKEAPDDA
                     TFARVTAPLWQYLDALHPYLWREGKDFPPSPARMDALLKAGTLRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFREGMIGNVHFVTIPANANASAAAKVVANFLLSPDAQLRKADPVV
                     WDDPSVLDPQKLPDGQREILQSRMPQDLPPVLAEPHAGWVNALEQEWLRRYGTH"
     gene            complement(7965..8513)
                     /locus_tag="OO130_003365"
     CDS             complement(7965..8513)
                     /locus_tag="OO130_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HCR5366643.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            complement(8513..9220)
                     /locus_tag="OO130_003366"
     CDS             complement(8513..9220)
                     /locus_tag="OO130_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCR5366644.1"
                     /translation="MMKMQSRKIWYYCITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            complement(9235..9912)
                     /gene="ydjY"
                     /locus_tag="OO130_003367"
                     /pseudo
     CDS             complement(9235..9912)
                     /gene="ydjY"
                     /locus_tag="OO130_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjY"
     gene            complement(9917..>10058)
                     /locus_tag="OO130_003368"
     CDS             complement(9917..>10058)
                     /locus_tag="OO130_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCR5366645.1"
                     /translation="RFILQLCLAGLALFILVQLAKLYARHKHVDLSASRRSPLTHPKN
                     EG"
BASE COUNT         2404 a         2742 c         2594 g         2318 t
ORIGIN      
        1 caagcacctt aaaatcacca tcatacacta aatcagtaag ttggcagcat taccggcata
       61 tctgctgcta tcgcagaaag ttaacggatg ctgaacttga agcgtgttat cgttacgaca
      121 agcagttttg ggagcactac agtaagaaat gccgttggcc cataagaaat gttccctggg
      181 gagcatcatc cgttaaatac tcataaacgc ctgaaatttt gccgggggcg ctgcgcttgc
      241 ccggcctaca ttagagcgta actgcaatga attgtgccca tttgtaggcc tgataagcgt
      301 agcgccatca ggcatttaca acttaaatca caccctgcgc cagcatcgca tcggcaacct
      361 tcacaaaacc ggcaatgttc gcgccctgca cgtagttggt ttgctcacct tcaccaccat
      421 gctcaacaca ggcatggtgg atatccagca tgatgtgatg caaacgtgcg tcaactttct
      481 cggctttcca gcccaggcgc gcagcgtttt gcgccatttc aaggcctgac gttgctacac
      541 cgccagcatt agccgcttta cccggtgcaa acagtacgcc tgcctgctgg aacagttcag
      601 tcgcttcgat ggtggtcggc atatttgccc cttcggcgac ggctttaacg ccattggcga
      661 taagctgatg cgcggcgcca acatccagtt cgttctgggt ggcgcaaggc agggcgatat
      721 caaccggtac agaccacggc tgttggcctt cgagatagac cagaccaaat tctttggcgt
      781 aatctgccac tcgaccatcg cggctggctt tgatttcgat aagacgtgcc agtttctctt
      841 tcgtgaatcc gctttcatca actacagtgc cgctggagtc tgacgcagtg atcacacgag
      901 caccaaattc catcgctttt tcgatagcgt actgggcgac gttgccggag ccagaaacgg
      961 aaacgcgcat cccttcaaaa cccataccgt ggcgttttaa cattgcttct gtgaaataaa
     1021 ccagaccgta gccggtagct tccgggcgaa taagactacc gccaaatgaa aggcccttac
     1081 cggtgaagac gcaggcggta ttgttggaga gctttttcat catccccgcc ataaagccga
     1141 cttcacgacc accaacgccg atatcacctg ccggaacgtc agtatccgcg cccagatgac
     1201 gataaagctc ggtcatcagc gcctggcaga aacgcatcac ttcgctttcg cttttacctt
     1261 tcgggtcaaa atcgctaccg cctttaccac cgcccatcgg cagggtagtc agggcatttt
     1321 tgaaggtttg ttcgaagccg aggaatttga gaatggagag gttaactgac ggatggaagc
     1381 gcataccgcc tttgtacggg ccgatggctg agctgaactg cacacgccat gcacggttga
     1441 cctgtacctg gttgcgatca tcaacccata ccacgcgaaa ctggatcacg cgctccggtt
     1501 caaccagacg ctccagtaat gacatctggc gatattttgg attttgttca agaaaaggcc
     1561 agagtgtggt cattacttca cgaacggctt gcgcgaactc ggtttgattc gggtcgcgct
     1621 tttggacatg gttgaggaat gactccagag aatatgtctg atccatagat ataaaaccct
     1681 tatatattaa tacgattgtg cttatgttgt ttatgtcatg tgcttttgca gttttcgact
     1741 ataccatcaa gaatgtgacg gtaagcaaga aaaaaatgac ccaggaaagc aaattaataa
     1801 cgagagtaat ctcataacca aatgcgataa ctcagacgct aaagttttgc cacccggcta
     1861 ccgttatcag ttgcagagac aataacgtta aggagttccc tgtgagtcga gcattgttcg
     1921 ccgtagtatt agcatttcca ttaattgcac tggctaaccc gcattatcgt ccggacgtgg
     1981 aggtaaacgt accgccagaa gtgttcagtt caggcgggca gtcagcgcag ccctgtactc
     2041 agtgctgcgt ttatcaggat caaaattatt ctgaaggggc ggtaattaaa gccgaaggaa
     2101 tactgctgca atgccagcgg gatgacaaaa cccttagcac taatccgctg ctctggcggc
     2161 gcgtaaagcc ataaacgcct ctaatagtgg aatgtcagta ggggccagcg gatattgcag
     2221 cgcctcttca ggtgagcacc agaccagcgc ctgatgttca tgtgcctgta acgtcccgtg
     2281 gaagtcgggt acgtgccagg catgaagatg gataatccgg cccgaaactt ctcgctgatg
     2341 gctggcaaca tactcaccca cagttgcttc gatgcccaat tcttcgttta actcacgcac
     2401 cagtgcctgc tgctggcttt catccagctc gactttacca ccggcaaact cccataatcc
     2461 cgcctgatcg ctctgggcgg ggcgttgcgc gagtaaaatt ttgccatcac gttcaatgat
     2521 ggcggcaaca acttcaatca ttttcataaa ttacacacgg gcagataagc gaaaaatgcc
     2581 ataatatccg cactgttgct caatcagaaa ggctttatat ttaaggagtg cctgtgctag
     2641 accgccatct tcatccccgg attaaaccgt tgttgcatca gtgcgtgcgg gttcttgata
     2701 aaccgggcat tacgccagat ggtttaacat tagtgggatt tgccatcggc gtgctggctc
     2761 tgccgtttct ggcgctgggc tggtatctgg cggcgctagt cgtcattttg ttgaacaggc
     2821 tgcttgatgg tctggacggt gcgctggcgc ggcgcagaga gcttaccgac gcgggcggct
     2881 ttcttgatat ttctctcgat tttctctttt acgcgctggt gccgtttggc tttattcttg
     2941 ctgcaccgga gcaaaatgcg ctggcaggcg gctggttgtt gtttgcgttt atcagcacgg
     3001 gcagcagttt tctcgccttt gctgcactgg cagcaaaaca tcagattgat aaccccggtt
     3061 atgcacataa gtcgttttac tatctggggg gattaaccga aggcacagaa acgatcttac
     3121 tgtttgtgct gggatgttta ttccctgcat ggtttgcgtg gatcttcggt gcgttgtgct
     3181 ggatgacgac ctttacgcgg gtgtggagtg ggtatctgac gctgaagtcg ctccagcgtc
     3241 agtaattgca atgttatttg ctactgtccg ggccgcgttc accggttgct accggatttt
     3301 ttggatcgct gctccattcg taccagccgc cgtcatacac ggaaacattc ttccaaccca
     3361 tggcgcgtgc gtacataaag gtttcggacg cgcgccagcc ggtgccgcag tagaatgaaa
     3421 cttgctgctc tggtttgata ttccacgctt tccacatagc ggtaatatca tcggcgctgc
     3481 gcatggtgcc atccgggtta tgaaaatctt ccatatgcgt cgagtcgcta ccagcgtgtc
     3541 cccaacgtgc tccggctatt tcaccttttg gtttaatata gctgtaaccg ctggtcgtac
     3601 cgataaattc tggccacgaa cgaatgctca ccagcgatgc atcctggcga tgcagcagtc
     3661 cacgggcctg ttccatatcg agcatcaact gcggttgtgc cgggatcttc acgccgaaat
     3721 ccggttccgc tttcactttc ggtggcgttc ctcgctcaac aggcagtccc gcgtcggacc
     3781 aggtttgcca gccgccatcc agcagacgca catctttcac gcctgcgtag agcataattt
     3841 gcgccacacg cgctccagcg tatacgtcac gcccgtacag aatgaccgtg gtgtcatggc
     3901 gaatgccgtg ttttgccagc atcgctttta gttgttcatc agaaactttg ttccacagcg
     3961 gttcgctttc cacttcgttg gtatcgatgt agtcagcacc gggaatatgg ctgataaggt
     4021 aaagcttagg agcgccccag gccgcttcaa tgactttcca gtcaccggca ggtttcgccg
     4081 taacctcttt accttgttgc aggtcgtgca gccattgcgg ataaaccagc tgctcaaagt
     4141 gcggcagttt ttgtagacgg gaaggttcgc ttagcgcgtc actcagaatg gagatatgcg
     4201 taaaacctgc tttttgcagt cgcgttttga cggcgtcgac atctttgtca ttaccgtaca
     4261 gcgccaccgg ggcatcggct ttcaggttat gttgcttgat ccacgcgttg agctgttcgg
     4321 tgctcatttt gtcgagccag ctggcagaga ggtttaaggc ggcaggttca tgaccagaag
     4381 ggccatttaa ggtttgtggc cagccgttat aaaacgcgct ggggcgagta tctatcgctt
     4441 tgccattttg ttgttgaagc tggtcaagtg taagaggctt cgccagttca gcggcccacg
     4501 aagaagcgca agccagccct aaagccattg ccagcgcggt catttgagaa acacgtttca
     4561 tcgaaaatcc cgacattgta gttttgagtg cgccattctg aactgacgca ttgagaaaaa
     4621 ctttgcgtta tcgcagtttg ttgtaatttt ctgaccactg cgccatatcc agaacagaac
     4681 tatcagcagg aacatcctgg agatcgtgcg ttacctgaac gacggggatc gccagttcgc
     4741 gaacttcgct gaacacccac tggcgaaaat tatcgcgcag agccacatca agacggctga
     4801 atggctcatc caggagtaac gcttttggct gagcgagaag ggcgcgcagc agggccaccc
     4861 gcgctcgctg accgcctgat aatgtcgcag ggtcctggtg ataagtttca gccagtcccg
     4921 ctcgatcaag cgcgtctttc acggcattgc gcctggcagt ccccttgagc gtcgacggta
     4981 gcgccagcag taaattttgc ccgacgctga actggtcaaa taacagtgca tcctggaaaa
     5041 gaatgccaat ctgacgctgt gcggtgggta gcatgtcaat ccgttgctca ttgagccata
     5101 gctcacctgt acaagaaaac tgtccagcca gtgcaccaat catccatgaa aacagagtgg
     5161 attttccaca gccagacggc cccattaacg tgacgatgtc acctttatca accgtaaagt
     5221 taacgtttgt cagcaagcgg ctttctggta aacgtagcga aacatttttc acgcagagca
     5281 ttagcggagt ccttgtctga cataacctac ccattttgcg gctaacgcgg tcagggcaaa
     5341 aataataagc ggtaacaaca gttgccataa agcctgggcg gcgagaatac cgttgctgcc
     5401 gccgctgctt aatgccaccg cctcagtggt gagcgtcgga aaacgccccg cgcccagcca
     5461 cagcgtcggc atatactgcg caatactgac tgaaaatccc accgcgaagg caatcagcac
     5521 agggcgcaac atgagtgtgc atttcacgta aaagaagatt ttggcccgcg accagcccag
     5581 tgtttgcgca atcaaaatta accgtgaatc aatgcgctgc caggcgggtt gcaggataaa
     5641 cagcatccac ggcattaccc acagcagatg cccccagacc accgccgtcc agcttccatc
     5701 caggttcagc catagcgcca gcgtgtactg gcccgccacc agtggcagag cgggcaatag
     5761 aatgggcaac catagccaca actggcgacg ctgtggcccc cattccagcc acagcagtaa
     5821 caggagcaaa gcgatgaatg ttgctaccag ccccattgtc agactgttga tgagtgcttc
     5881 actattgatc gtcgactgat ccgcgaggat cgccagcaga accacacaca gcacaccggt
     5941 taagggtaaa aaactcgcca gcgtagtgcc tggtaataaa ggtgtggcag gcttgcgaat
     6001 gccatctacg cggggaatag tgcgccgcca gctgcgccat agcagatagc tcagcaaaac
     6061 gtaggcggcg agtaacagca tcagcagcag actggcgagt gcgcctttcg tttgttgatc
     6121 cgcgtcgccc tgagttaacc actgccagct aattactgcc agcgtcggtg gattacctgg
     6181 cccaagaata atcgccacat ccacgaccga aagcgaccag gcaacaatcg ccagcatcgc
     6241 cattgccagc gaaggcgcga cggagggcaa cagcagccag ttcaggcatt gccagcggct
     6301 gtagcccagc gaatccagca caatgacctg ctgcaacagc cgtttttcgc tcaatactgc
     6361 cgctaagatc cacagcagaa atgcgctttc tttcactgca agggtgaggc ccagaccgat
     6421 gccaaatcgg tccattggcg gagtgaaata cgggaaatag tcatatagca gccctccgtc
     6481 agcaaagagc agcagggcgc tggtggcaaa agccacatgg ggaatggcga gcagccacgg
     6541 cagacgggcg cacatacgct gccatttcgg cccaggccat agcgccacaa tcaccagcag
     6601 ggcaatcaac aatgccccga ccgccgcgat ggttgtcgac accagcgttg ccagtaatgc
     6661 atgcggtaac tgtggatcgg caaataacgc ctgccagtgt gtcaacgaca acgcaggcga
     6721 tatcagcgtg agagctgccg ggatcagcgg tgcataaatc accgccacca tcgcccacag
     6781 caaaaagatt aatgcgtacc gtaacggcgt aaccattctt gttccagtgc attcacccat
     6841 cctgcgtgcg gttcagccag taccggcggc agatcctgcg gcattcttga ttgcaatatt
     6901 tcgcgctgcc cgtcaggcag tttttgcgga tcgagaacag aaggatcgtc ccagacaacg
     6961 ggatctgctt tacgcagttg cgcatcgggt gagagcagaa aattggcaac taccttcgcc
     7021 gcagcactgg cattcgcgtt ggctggaatg gtgacgaaat gcacgttgcc aatcatcccc
     7081 tcgcgaaagc caaaactgta actgcttgcc ggcaaatcgc cgctggcgat tttttgctgc
     7141 gcatgcgcgg ggttaaaggt cagcgacagg cgcaatgtgc cggctttcag cagagcatcc
     7201 atccgcgcgg gcgaaggcgg gaaatccttt ccttcgcgcc acaaatacgg atgcagtgca
     7261 tcaagatatt gccacaaggg cgcagtgaca cgggcgaaag tcgcatcgtc cggcgcttct
     7321 tttaatgcgg cgggatctgg cgtcagcata atcagcaact gttcaagaaa cgccgtgccg
     7381 gtaaagtccg gtgggcgcgg ataagtaacc gtgccgggat tggctttagc aaactccagt
     7441 aaggcttgcg gcgtttgtgg tggctgtggc gtaacatcgc ggcgggcgat aaacgtcagt
     7501 tgtgcgccgc cccagggcga ttccgcacct tgtgtcggca ctgaaaaatc ttcccgcacc
     7561 ggcagctgtg tgtcgacata gcgccagttg ggcagagtct ccgcccagcc cgtttgtaat
     7621 aaattggcct cttttaaggt gcggaagttt tcgccgttca cccagagcag atccaccgag
     7681 ccgcccgttt tacgaccggc agcagcttcg gtctgaatgc gcttcaccgc gtctgcggca
     7741 tccgcgagac gaacaatctt caggtttata gcgtaatggg ttttcatctc gccgctaacc
     7801 cagtcgagat agcggttaat tgcggtatcg ccgccccagg cgttaaacca gacggtttgc
     7861 cctttggcct catttttaat ttcctgccag tcactggcat gtgttgccaa agaaaacagc
     7921 gataacaatc ccagcaacca cccacaatgg cgcatcttct ctccttaaga acgtttttcc
     7981 ggaatacggc acagcccctg agcgggaatg tccatggcag aattaaaacg agccgacagg
     8041 ttttgcaggc cgatcaatgc cgtcagttcg gtgagcgcct gagcgtcaaa atgcgcagcc
     8101 agtcgggtac gcagggcatc atcgactgtt ggcggtgtga cgcttgcggc ttcggcgtac
     8161 tccagcgcca gccgttcttc atcgctaaag agcgggcttt gccgccagtc agccactgcc
     8221 agcagtttat cggtgctgcc ggtacgctcg gcgactttca tactggtgat atccacacaa
     8281 aactcacaca ggcacatttg cgcaatgcgc gcgctgacaa gcgatcgcac taccggatcg
     8341 agcggtgagc gtttgcgctc cagccaccca acaaacattg acaccaaata aaagataaac
     8401 gggatccgcc cccaccagcg aatcgggctt agcacctcgc cgtaatggcg acgatgcagc
     8461 cacgcctgcg gacgtaaaat gaaaggaatt ttgctaagcg gcggtaaacc catcagtcat
     8521 tcctcttctg gcgctcaagc catattttct tcgccataaa aatcaccacg gtcagggcaa
     8581 acagaataaa cagtccggtg acaaaccaga aagtaccgcc tgttaacatg ctgcccgccc
     8641 aggaataaac aatagtcgcc ggtaattgac caagcccggt ggcgataaaa aacgagcgaa
     8701 aacgtattga agtcagaccg gcagcatagc tgattggatc gaaagggaca aaaggcaata
     8761 accgacagac caggatggtg tgtttgccgt agcgagtgaa aaagccgtcc atactgtcaa
     8821 gcacggtttt gccggttaat ttttccacca cttcgcggcc catcactctg gcgataaaaa
     8881 agcacagcgc cgcgccggcc atcgaactgg tccacgacag caagccgccc cagaacgcgc
     8941 caaacagcga cgcattggca aaggtgatca aaaacgcagg tagcggtgca gcaatggcct
     9001 gtaaaatcat caacaagaac gagacaactg ccgccagtgc gccgtaagac tgaataaagc
     9061 gttctatacc ctgttggtcc acggcggcaa acgccgcaac gctgcgattg ataaactcat
     9121 ggacgccggg aagcagcgcc catgcgagca gcatagcgaa caacaggatg atgagggtta
     9181 tacagtagta ccagatttta cgcgattgca tcttcatcat cctttatcct ggctttattc
     9241 ggtgatttta aaggtaaccg ttgccagcgt gttatccgcc gggagaactg aggcattgcc
     9301 tttgaatttc acttcgccgc gtttttccac cgcaccataa gtgtatgttg cattgctgac
     9361 gatgccgacc gggcagctat ccagacacac cagtcaaccc gttttcttct cttctgctgc
     9421 cgttaaatta ccgccaaagc gcatgtccag tttcttgcca ttactgtcaa caatcacttc
     9481 atcgaaggaa tacgcttttg ccgccccttg ccagttgacc gaaatatcca gtttgctgcc
     9541 tgtgacatga gtcgtttctt tattatccat cgtcatgttt tcgcccggcg taccacctgc
     9601 ctctttcagg gcttcataaa atgctttcgg ggtcgcataa gccataaaca gcgatttatg
     9661 tccgttggag ccatctttaa acacaatacc gtgacgagtg tcgtcggtga ggaaacgacc
     9721 attaatttgc accagcatgg ttaccgtatg gttattggca tcgacgcgca gcggttggct
     9781 gttcgaaagt ccgtcgtatt ccacttttgc cgccgaattc tctttttgat cgcagccgct
     9841 aagcccggca acagccagta agcagagtgc agccagtcct tttttccatg acactgaata
     9901 atgttgcgac atcgttctat ccttcatttt ttgggtgagt aagcgggctg cggcgcgaag
     9961 cagacagatc cacatgtttg tgtcgggcgt agagttttgc gagctggacg agaataaaca
    10021 gcgccaggcc cgccagacag agttgtaaaa taaagcgc
//
LOCUS       DAJTMC010000153        10005 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.153, whole genome shotgun sequence.
ACCESSION   DAJTMC010000153 DAJTMC010000000
VERSION     DAJTMC010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10005
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.153"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(272..1636)
                     /gene="tdcG"
                     /locus_tag="OO130_003369"
     CDS             complement(272..1636)
                     /gene="tdcG"
                     /locus_tag="OO130_003369"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCR5366646.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGALSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(1708..2097)
                     /locus_tag="OO130_003370"
     CDS             complement(1708..2097)
                     /locus_tag="OO130_003370"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HCR5366647.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(2111..4405)
                     /gene="tdcE"
                     /locus_tag="OO130_003371"
     CDS             complement(2111..4405)
                     /gene="tdcE"
                     /locus_tag="OO130_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HCR5366648.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFEAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNIMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(4439..5647)
                     /gene="tdcD"
                     /locus_tag="OO130_003372"
     CDS             complement(4439..5647)
                     /gene="tdcD"
                     /locus_tag="OO130_003372"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HCR5366649.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSCGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(5673..7004)
                     /gene="tdcC"
                     /locus_tag="OO130_003373"
     CDS             complement(5673..7004)
                     /gene="tdcC"
                     /locus_tag="OO130_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HCR5366650.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTISMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(7026..8015)
                     /gene="tdcB"
                     /locus_tag="OO130_003374"
     CDS             complement(7026..8015)
                     /gene="tdcB"
                     /locus_tag="OO130_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HCR5366651.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(8114..9052)
                     /gene="tdcA"
                     /locus_tag="OO130_003375"
     CDS             complement(8114..9052)
                     /gene="tdcA"
                     /locus_tag="OO130_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HCR5366652.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRLLIEVG"
     gene            9241..9585
                     /gene="tdcR"
                     /locus_tag="OO130_003376"
     CDS             9241..9585
                     /gene="tdcR"
                     /locus_tag="OO130_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HCR5366653.1"
                     /translation="MTGITIFYGDNIIRYVVNTKKGLRPYFKQLPDNYQAKFELNLMS
                     KFSNFIINKPFSAINTAARHIFSRYLLENKHLFYQYFKISNTGIDHLEQLINVNFFSS
                     DRTSFCECNRFP"
BASE COUNT         2696 a         2516 c         2274 g         2519 t
ORIGIN      
        1 tttctcgctc aatattttgt agggcttatt atttttttcc gagccgcatc aaggcgatat
       61 gcggtctacg tttttgcagg cgagttatta gaataaagaa atgatacgcg cgaggtcaga
      121 gaaaatgttc acaatcaaga ctttgaaata acggagatgg ataagaattt tctactaaat
      181 taatcgcaag agaatattca ttctctattt gcggcagatc acaaaaaaag gtgcacattt
      241 gtgcacccaa ggatgaaagc tgacagcaat gtcagccgca gaccacttta atggccagtc
      301 ctccgcgtga tgtttcgcgg tatttatcgt tcatatcttt gccggtttca tacatcgtct
      361 cgatcacttt atcgagtgaa acacgcggtg ccgaggtgcg gcgcatcgcc atccgcgcgg
      421 cgtttactgc tttcacggca ttaatggcat tacgttcaat gcacgggatt tgtacctgtc
      481 cggcaaccgg atcgcaggtc agcccaaggt tatgctccat cgcgatttcc gccgcattgc
      541 atacctgcgc cggactgccg cccagtagtt cagttaaccc tgccgccgcc attgaacagg
      601 ccacgccaat ctccccctga cagccgacct ccgcgccaga gatggaggcg ttcattttat
      661 acagcgcgcc aatagccccc gctgccagaa aatagcgggc aattgaccgc tcgtttaccg
      721 gacgacggaa cttatcgtaa taagccagca ctgccggaat aatgccgcac gcaccgttag
      781 tcggtgccgt taccacgcgc ccgccagctg cgttttcttc actaaccgcc agcgcgtaca
      841 tgttgatcca gtcgatgaca ttcatcggat cgttagagat gttatcgcta gaaaccagct
      901 gacgacgcag cgctacggca cgacgcggca cattgagcgg accaggcagc acgccttcag
      961 tgttcatccc gcgttcaata ccgtcatgca tcacttgcca gatacgggca aaccctgcgt
     1021 caatttccgc tttgctgcgc agcgccagct cgttgtgcat catcaggcca gatatagaca
     1081 ggccgttgta atcacacatt ttcagcagtt cacctgctga gtggaaatcg taaggtacgg
     1141 acgtttcgac atcgtgcgac aggccgaagt gttcttcttc gacaataaac ccgccgccga
     1201 cagagtaata ggttttactt aatagcgctt cctgtccctt ccaggcagtg atccgcattc
     1261 cgttctcatg gcgaggcaac atttcgggat ggaagataat gttctttgct acaggaaaat
     1321 caacaatatg cgcaccagat gccactggca gccgcccgct gcgcgttact aactctataa
     1381 atgcagggat ctcatcaatg acaacatcct gcggactgtt tcctgccagt cccatgataa
     1441 tggcgacatc cgtggcatgg cctttgcccg tcagtgacaa cgccccatac agatcgacca
     1501 caatatggct cgtcgcggtt aataagccgc tactttccag ccgatcaata aaactttttc
     1561 cggcattcat tggccccacg gtatgcgaac tggagggacc aatcccaatt ttgaaaatat
     1621 cgaatgcact aatcatatcc acaccctcgg attgccgttc agtgaagtgg agcggaacga
     1681 ccttgcgacc gtcccgctca cgaggcttta cgcactacgt actgcgatag cttcaatttc
     1741 cagcttcaca tctttcggca aacgcgcaac ctgcacacag ctccgggtcg ggtaggtcgc
     1801 ctgatgctca tcgaagaact gcttatagac ttcattgatg gtggcaaaat cattcagatc
     1861 ggtgatgaac acggtcatct tgatgatatc gcccacgctc agcccggcag caaccacgat
     1921 cgctttgacg ttttcgaggc ttaaacgcgc ctgatcttgc acatcagccg ggatctcacc
     1981 ggtctgcggg caaaccggaa tttgcccgga ggtgaagacc atgctgccta aatcaacgcc
     2041 ctgaacataa gggccgattg cgcctggggc acgttgcgtt tcgataatct ttttcataca
     2101 tcctccggcg tcagagcgcc tgggtaaaag tacgtgaaat aacatcctgt tgctgttcac
     2161 gggtcagtgc gttgaagcgc acggcgtagc cagagacacg gattgtcagg ttaggatatt
     2221 tttccgggtg ctcgatggca tccagcagca tttcccgatt cattatgttg acgttgaggt
     2281 gttgaccgcc ttcgacatcc gcttcgtggt ggaaataccc atccagcagg ccgacaaggt
     2341 tagttttacg tactggatct tctttgccca gcgccgcagg aacgattgag aaggtgtacg
     2401 agatcccatc tttggcgtag gtgaacggca gtttcgccac cgacgtcaat gaggccacgg
     2461 cacctttgcg gtcacgacca tgcatcgggt tagcgcccgg cgcgaacggt gttccggcgc
     2521 gacgaccgtc cggcgtatta ccggttttct ggccgtacac cacgttagaa gtgatagtca
     2581 gaatcgactg ggtagggacg gcgttgcgat aggttggcag cgctttaatt ttcttcataa
     2641 agcgttcaac caggtcgcag gcaatgctgt ctacgcgctc gtcgttgttg ccgtactgcg
     2701 gatattcacc gtcgatttca aagtccaccg ccaggccgtt ttcgtcacgg attggtttca
     2761 cgcgggcata tttgatggca gacagggagt ccgtcgccac cgacaggccc gcgatgccgc
     2821 atgccatagt gcgatagaca tcacgatcgt gcagcgccat cagcgaagct tcgtagctgt
     2881 acttgtcgtg catgtagtga atgatattca gcgcgctgat gtactgcacc gccagccagt
     2941 ccatgaagtg atcgaggctg tccatcactt tgtcgtaatc cagcacgtcg tccatcagcg
     3001 gtgctgtttt cggcccgacc tgaatcttca gcttctcgtc caccccgccg ttaattgcgt
     3061 agagcagcgt tttcgccagg ttagcgcgtg caccaaagaa ctgcatttgc ttaccaatca
     3121 ccattgggct gacgcagcag gcaatcgcgt aatcgtcgct gttgaagtca gtacgcatca
     3181 gatcgtcatt ttcatactgc aaggaagagg tgacgatcga cacctgcgcg gcatattttt
     3241 tgaaggcaat cggtaattct tccgaccaaa gaatggtcag gttaggttcc ggtgccggcc
     3301 ccatagtgtg cagggtgtgc aaatagcgga aggagttttt ggtcaccagc gtacgaccgt
     3361 ccagccccat cccgccgatc acttccgtcg cccagattgg gtcgccggag aacagcgaat
     3421 caaattccgg tgtacgcagg aagcgtacca tacggatctt catgatgaag tgatcgatca
     3481 gttcctgtgc ctgctgctca ttgagtacgc cagcttcaaa gtcgcgctca atgtagatat
     3541 cgaggaacga tgccgtgcgg cccagcgaca tcgcgccgcc attttgcgat ttcactgccg
     3601 ccagataagc gaagtagagc cactgcaccg cttcttgcgc attctgcgcc gggcgagaga
     3661 tatcaaagcc atatttcgcc gccatttcct gaatctgcaa cagcgcatga cgatgctctg
     3721 ccagctcctc acgcagacgg atggtggctt ccagatcctc gcctttttcc agacgagact
     3781 ggagatcggc aaattgcagt tcgcgttcac gtaccagata actgatgcca tacagcgcta
     3841 cgcggcgata gtcaccgata atgcgcccac ggccatagcc atctggtaaa ccggtcagca
     3901 cgccagattt acggcagcgc agcatatccg gtgagtaaac atcaaatacg ccctggttat
     3961 gggttttacg cagatcggta aacagatatt caaattcact gtccatttca cggccatagg
     4021 cgtggaatga acttttaatc atattaatgc caccgaacgg gtgtagcgca cgtttcaacg
     4081 gcgcatccgt ttgcaggcca acaatttttt ccagcggctg gttaatatat cccgcatcat
     4141 gagcggtaat tgtggtggca atattggtat cgaaatcaac cggcgcgtgg gttgcgtttt
     4201 cgatacggat accttccatt actttttccc acaattccgt ggtggcaggc gtcgcttcgg
     4261 cgaggaaaga ttcatcgcct tcatacggtg tatagttatg ttgaataaaa tcgcggacat
     4321 taatttcgtt tttccagtcc gtacctttaa agccaagcca tgcgtcggcg tacagcttat
     4381 cgctggtatc aatatctacc ttcatgaaaa ataatctctc tacaatactt caactaaatt
     4441 atgcaaattc tgcgggcgcg ttaactttgc ctaaatgaat ggcatccaaa gcaatcattt
     4501 tttcttcgtt agtcggaata acagcgcaaa tgacacgcgc attttcactg gaaacaattc
     4561 gctcaccaca ggagttagag cgattattca tttctgtatc aatctctacg cctaatacag
     4621 ccaaatgttc catgaccaga cgacgaatta agcttgaatt ctctcctatt ccgccggtga
     4681 atataattcc atccaggcga cgtaatgaag ctgcgtgtcc ggcaatatga cgggcaattc
     4741 ggtgaacaaa ggttttaatt gccagttgcg cgcgttcgtg accttcatgc caggcttttt
     4801 ccagaacacg taaatccgaa gaaagaccgg aaatacctaa taatcccgac tctttattca
     4861 ctacgcgttc caagtcaccc aagctctggt tggtttggct ggcgacccag gacatcgcac
     4921 caaaatcgac atcgccactg cgggtaccca tcatcaagcc ttccagcggc gtcattccca
     4981 ttgaggtgtc aacactctga ccgttgcgaa ctgcgcagat tgacgcgcca ttgccaagat
     5041 gcgccacaac caggccggag tcatcttccg ctagattcag caaagaatgt gcgcgctggg
     5101 aaacatagcg gtgcgacgtg ccgtggaaac cataacggcg tacacctaac tcttcataat
     5161 atttccacgg caggccgtat aaataagctt ccggagccat cgtctggtgg aaactggtat
     5221 caaataccgc cacctgagtt acgcccggaa ataattgctg cgccgattca ataccactta
     5281 aattggcgta attatgcagg ggtgccagtg gagaaacgcg acggatatta tcaatgacct
     5341 catcggtaat aatggcggac tcggtaaaaa tactgccgcc gtgagcgatg cggtggccaa
     5401 ttaaggccac actgtcattt aaattccgtt tttccagttc aaatgcaatt gccttcaatg
     5461 caccttcgta gctgtggtga gccagcggtg ctggctctcc cccatttacg gataagaatg
     5521 cattttctga gttaataccg tcggcaatac ctgacattaa tacttcacag tcgctggcat
     5581 cgagtacgga aaacttaatc gaagacgaac cacagttaat aaccaaaaca accggaaatt
     5641 cattcatctc ttttctcatc ctgagttacg gattaaaaca gtttgtatac gatgttcagg
     5701 atggtcagca gaccaatcac ggtaacaaac acgttatcca gacgaccacg gtatttcgcc
     5761 agagacggtg ctttacggat ggcatacatc ggcaacaggc acagcaggga tgcgataatc
     5821 ggtgcgccca tggcttcaat aaggtcgagg atgttcgggt tggcgtaggc aacaacccag
     5881 gtggagccca tgatgaagat catgctgata gtgttcagtt tacccagcga cactttagtt
     5941 ttgtcgcctt tataaccgaa cttcagaatc agaccattca agccttccag cgtccccaga
     6001 tagtgaccga agaaagattt gaagatagcc acgagtgcga tgatggaagc cgcatattcc
     6061 agtgtaatcg cgaacgttgt tttggtaccg gtcatggacg caaagtggtt agccagataa
     6121 gaaagcactg gaatattctg cgctttggct tccgccatgt tggccggaga cagcgtaaac
     6181 aggcagctaa aggcaaagaa catcaccact gcaaccatca gcatgctggc acgagaaatg
     6241 atttgggaac atttacgttc ggtgaagtcg cgaccgaagt ctttctcata ctcttcacgt
     6301 ttagaaacca cgaaggaaga gacgattggc gagaagttaa aggagaaaac catgatggaa
     6361 atccccagcc agacagtgat caggataccg tcatgaccgg ttaacgacag cgaaccgagg
     6421 tcaacctggt cgataactgc agagttccag taagggatca gcgacagaga aatcagcacc
     6481 aggctggcga taaacggcca taccaggtag ctcatcactt taaccatcag atccttacca
     6541 aaccagatga cgaaagccat cagcagcaac aggaacagcg ccacaaagcc gcgattcagc
     6601 ggtgcaaatc cgagctggtt ttcccagaac gtcataaagg tattggtaat agtaacgcca
     6661 taaatccaca gcagtgggca aatcgcgaag aagtacagga acgtgataac cacgccgcca
     6721 gttttaccaa aatgctcttc caccgtttcc gtaatgttgc cggaagggtt agagccagaa
     6781 agacacagac gcgccagcgc ccggtggcaa taaaatgcga tggggtatgc caataccaac
     6841 atcagaagaa tcgggatcag tccgccaaaa cctgcgcgga tagggaagaa cagcaccccg
     6901 gcgccgattg ccgtaccaaa caagcctaac gtccatgtgg tatctgattt acgccaggac
     6961 gattgttttg tctggctgga tacaatgcta tctgaagtac tcatatccta tcctcaacga
     7021 attaattaag cgtcaacgaa accggtgatt tgagagacgc gagaaagatc gatattgccg
     7081 ccggaaataa tactgacggt ttttctgttt tgaatatatt ggtctaattt accgcttaat
     7141 aatgcagcac atgccagagc acctgcgcct tcggtgacga ctttattgcg ctgaattaag
     7201 gcaatcatac tgtttctgat ttcgtcttcg ctgaccagca cgatgtcatc gactaattca
     7261 cgaacgattt cgtaagttaa attacccggg cgggagacat cacaaccatc cgccagggtg
     7321 ccggtagttc ggtgcgtggt tatttctccg gagtggaaag aagccgccat gccgtgaacg
     7381 ttttctgact gtacgccaat aacacgaatg gtcgggttaa tagatttaat tgccaccgca
     7441 ataccagcaa ttaaaccacc accaccaatt ggcacaatca cgttatcgac atcatagaga
     7501 tcttccataa tttccagacc aatcgttccc tggccagcaa tcactttcgg atcatcgtaa
     7561 ggtgggataa aaatacgacc ttccatttcg acaatttcgc tcactttagc gatagtgtcg
     7621 ttgaagttat caccatgcag aacgacttct gcggagtagt cgcacgttgc cgctactttg
     7681 gattttggcg caccttttgg catcaccact ttaccgtcga tacccagcat cgcgcaggag
     7741 agcgaaaccc cttgcgcatg gttgcccgca gaacaggcca ccacgccttt gcgtttttcc
     7801 gcatcggtca gtgaacttaa tttattaaat gcgccacgaa ttttaaatga acccgtacgc
     7861 tgcatatttt caaacttcag gaatatttca cctttgcaac gttcactaaa atagttggag
     7921 cgaggcatgc ctgttttata aattcgccca gccagtcgtt gtttcgcttc aataatatca
     7981 tcaatagcaa ccggcagatc gtatgtaata tgcattataa aacctcttcg cctgaattaa
     8041 atatgtagat aaccgtaacc gacacctgca aaacagacag gtggattatt tatgtttaaa
     8101 taaaaacaaa taactaacca acttctatta atagccttcg tctacaccca ttataagatg
     8161 aatactcttt ggctaattcc accaaaaccg atgctgcttt tttaatacga taatttttcg
     8221 accataccgc ggcatattgt gccacaggta atgtttcttc aactggaata gtaataaatt
     8281 gatttgaacc aaaaggtgac gtcatatcac aaggaattac agttaagaaa tcagcattga
     8341 gaacaagatt ataaattgtc acgactgagt cggttttaac gatgttttca atactgatgc
     8401 catttctttg taacgtagta agaagctcgc tgtagtaccc catattggtt tgtggcaaca
     8461 cccactgttc gttcttcaac gactccagcg tggtggtgcc ggtgcatgtt cgggacttac
     8521 tggctaccag cacaaactcg gactcgaaca gcggctcaac atgtaaatcc tgaagcttca
     8581 tttctgcact taacgtacca atcgcaaaat ccagtcgacc gtcgcggatt gccggtaaga
     8641 acgaagacag ttgcgcttca tacatagaaa cctgcgcttt cgggaacacc tctttgaact
     8701 tgttgatcat ccctgacata aaagtaaaac caatcaatga aggaaaacca aatgagactt
     8761 ccaccaccgc ctcagaagac ataccgctta tctcattaac catatttttc atttcacggg
     8821 taatggattc ggaacgggag agtaacaatt gaccggcagg tgttaatgtt acaccggtat
     8881 ttttccgcac cactaattcc acaccaaaat aatcttcaat atcgttaatg attttactga
     8941 cggccggttg agttaaccct aattcttttg cagccgagcc gatagagcca cttctaatga
     9001 cttcctgaaa gactaccagg tgctgcgttt tcggaagaag aatagtgctc ataacgacct
     9061 acgttaatta cctcattgac ggcatgaagt gtatcaaaat gaaatgaaca ggatatgtgc
     9121 gaccactcac aaattaactt tcattacttt ccagggtatc gttattacaa ttaaatatct
     9181 ttaaatatca acaagttaaa acataaaaat cagcataaac cctgattttt atcaaaaaaa
     9241 atgacgggga taaccatatt ttatggcgat aacatcattc gttatgtggt taatacaaaa
     9301 aaggggctga gaccatattt caagcaatta ccggataatt accaggcaaa atttgagtta
     9361 aatttaatga gcaagttctc aaattttatt ataaataaac cattttcagc gataaatact
     9421 gcggcacgtc acattttttc acgctatttg ttggagaaca aacatttatt ttatcaatat
     9481 tttaaaattt cgaatacagg tattgatcat ctcgaacaat tgattaacgt caactttttc
     9541 tcttctgaca ggacgtcatt ttgtgaatgc aatcgttttc cataaattct tctcccctca
     9601 taggcgacga atagcatttt gtgttgagga tcacaaaaca aataattgct ggtcgccgcg
     9661 ataaggtcag acgaagtcaa caaggggaat tttcactgtg ctttacatca gcgtaggaca
     9721 cagaatgttg cttcgtttac tgtaacgccg ggtaaatgag cgttttttga tagtgcgtaa
     9781 gtgtcgtgcg aggaaaataa gcatctatta ttgtttgtca tgacgacaag gcaggcatca
     9841 atgaaaggct tcccaattgc acatattttt cacccttcaa tcccgccaat gcacgcagtg
     9901 gttaacaatc acaatagaaa tattgattat tggacggtaa aaagaaagtt tgcagaaatt
     9961 gtctccacca atgacgttaa aaaaatttac agtataagta atgaa
//
LOCUS       DAJTMC010000154         9956 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.154, whole genome shotgun sequence.
ACCESSION   DAJTMC010000154 DAJTMC010000000
VERSION     DAJTMC010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9956
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.154"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            260..457
                     /locus_tag="OO130_003377"
     CDS             260..457
                     /locus_tag="OO130_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5366654.1"
                     /translation="MEITPQQLVEFTIPDGRGQQARTLPDMLSSDAGPLSYPLIYSSK
                     VMIISLFDSTVMPDKYRHNGK"
     gene            complement(407..1600)
                     /locus_tag="OO130_003378"
     CDS             complement(407..1600)
                     /locus_tag="OO130_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5366655.1"
                     /translation="MSPGIEDTPQKTLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFVTDTTAISQQVAWLLGLSAVAALIAQFGV
                     VRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            1736..3460
                     /gene="iucA"
                     /locus_tag="OO130_003379"
     CDS             1736..3460
                     /gene="iucA"
                     /locus_tag="OO130_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HCR5366656.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            3461..4408
                     /gene="iucB"
                     /locus_tag="OO130_003380"
     CDS             3461..4408
                     /gene="iucB"
                     /locus_tag="OO130_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase"
                     /protein_id="HCR5366657.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            4408..6150
                     /gene="iucC"
                     /locus_tag="OO130_003381"
     CDS             4408..6150
                     /gene="iucC"
                     /locus_tag="OO130_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucC"
                     /protein_id="HCR5366658.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFVEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDATSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            6147..7484
                     /gene="iucD"
                     /locus_tag="OO130_003382"
     CDS             6147..7484
                     /gene="iucD"
                     /locus_tag="OO130_003382"
                     /EC_number="1.14.13.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase"
                     /protein_id="HCR5366659.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFARNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVITLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSRKKPQPEAASLTHYTANIQE"
     gene            7490..9685
                     /gene="iutA"
                     /locus_tag="OO130_003383"
     CDS             7490..9685
                     /gene="iutA"
                     /locus_tag="OO130_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HCR5366660.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWIIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IAAGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLSLTKSVNVSDSKLEGVKVDSYELGWRFTGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
BASE COUNT         2396 a         2564 c         2644 g         2352 t
ORIGIN      
        1 tcataaaaaa catgtcagtg aaaaagaaac ctggcgttgg gttgaaggat ataataaagc
       61 aacagaactg gctcgccaat gccctgatac ccatgtattg tcaatttctg atcgtgaagg
      121 cgatttttat gacctgtttg aacgcgcaga gcagactccc ggaataaaag ctgactggct
      181 tgtcagaatg aaatttaata acagagcaac gctgaatatt aatggtaaac gggatcatag
      241 gttgttgcac gaaagaatca tggagattac accgcagcaa ctggttgaat tcactatacc
      301 tgatgggcgg gggcagcaag ccagaacact gcctgacatg ctttcatcag acgcaggtcc
      361 tctctcatat cctctgattt actccagtaa ggtgatgatc atatctttat ttgatagtac
      421 ggtaatgcca gataaataca gacacaatgg taaataatat agttgcaaat aatgctgcca
      481 taatcagcgc gattgcgaca cctgttttcg ataccagcgg caccagcaaa gcgcacagtc
      541 catagccaag tgtgtgactg gtagcgagcc agcccgcgcc tgcgccatca gccagcttat
      601 cattcagtag aagttgataa gcgggtgttg ccagagctgc tccaaatgac agcactgcac
      661 agcccgggta aaacaaccat agctgttcgg agagcatgat agccagacca ccactcatca
      721 acactccggc actcaggagc agggccaccg gagtcaggcg ctgcggacgg actaccccga
      781 actgcgcgat aagcgcagct actgcggaca gacccaacag ccaggccacc tgttggctaa
      841 tggcggtggt atccgtgacg aactggcgag taagggcagg cgaaagtcca agctgcatca
      901 tgctcaccgc cgcagccagt aatattgcgc aaagcagata aggcagacaa tcccgcttca
      961 gactgacgct cttgcactcc ggtgtgggct gtggtggtgt gccgggcaac cgcagcagca
     1021 tcagcagcgc cagcactggt gctgccatca gtagccccag tggcgccaga ggatgaatgg
     1081 ccagcattgc tgccgcgcac agcggcccga atagcctgcc gcaactcagg ccggagctga
     1141 tggttgccag agcggccatg cgattccctt ctccagctct ctgcaatgcc cagacctgac
     1201 aggctggcac cattgccgac acggtcagcc cgtaggcgat ccgggcgatg attaatattc
     1261 ccagtcccac cgctgttgtt atggcgctgg tagccatcag cacgcttccc agccctagca
     1321 gggtaaagct tatgccatat cctcccagag cccacaacac cacaggttta ctgccagtac
     1381 gggaaatttg ctttccccac cacggggacg atggcagaaa cagcattgag ccaagcatca
     1441 gcagggcagc ccatacagac agactcagat ttgtctgtat gaccagaaca gggagcacaa
     1501 ccagcagacc gttctgtccg ataccgagaa gcccggcact gaacgcaagt ggccagcagg
     1561 acagtgtttt ttggggcgta tcttcgatcc ctggtgacat tataatgctt caactccatg
     1621 tattaattgt gtttatttgt aaaattaatt tatctgacaa taacatttat tgttgataat
     1681 gagaatcatt atcgacataa ttgttattat tttactgtgt gggagctgtt tgactatgac
     1741 cctgccctct gaaaaaccag ccacagatgt ggctgcgcag tgcttcctga atgcactgat
     1801 tcgtgaaacc acagactgga aactgacaga atacccgcca gacgaattgc tcatcccgct
     1861 ggatgagcag aaatcgctcc atttcagagt ggcttatttc tccccaaccc agcatcaccg
     1921 ctttgcattt cctgcacgtc tggtcacggc atcaggcagt tatcctgtcg actttaccac
     1981 tctctcccgg cttattattg ataagctacg ccatcaactg tttctgcccg ttcccctctg
     2041 cgaaactttc caccagcgcg tgctggaaag ccatgtccat acgcaacagg caattgatgc
     2101 ccgtcatgac tgggccgctc tgcgtgaaaa agcgttgaat tttggcgagg ctgagcaggc
     2161 gctgctgaca ggacacgctt tccaccctgc gcctaagtct catgaaccgt ttaaccggcg
     2221 ggaggctgaa cgctacctgc ctgatatggc accccacttc ccactgcggt ggttttcggt
     2281 ggataaaacg caaatcgccg gtgaaagttt gcatctcaac cttcaacagc ggctgacgcg
     2341 atttgccgca gagaatgcgc ctcagttact caacgaatta agtgacaacc aatggctgtt
     2401 cccgttgcac ccgtggcagg gggaatatct tttgcagcag gggtggtgcc aggcacttgt
     2461 tgctaaaggg ctgattaaag acttaggtga ggccggcacg tcgtggctgc cgaccacctc
     2521 ttcccgttcc ctctactgtg ccaccagccg cgatatgatc aagttctccc tgagcgtacg
     2581 gctgaccaac tccatccgta ctctgtccgt gaaagaagtg aagcgtggga tgcgcctggc
     2641 acgcctggct caaaccgacg gctggcagat gctacaggtc cgcttcccga ctttccgggt
     2701 aatgcaggag gatggctggg ccgggctgct cgatcttaac ggcaacatca tgcaggaaag
     2761 tctgtttgcc ctgcgtgaaa atctgctggt ggatcagccg aaaagccaga ctaacgtact
     2821 ggtctccctg actcaggccg cacctgatgg cggtgattcg ctgctggttt cggcggtaaa
     2881 acgcctgagc gatcgcctcg gtatcactgt gcaacaggcc gcccatgcat gggtcgatgc
     2941 atactgtcag caagtgctaa agccgctgtt tacggctgaa gcggattacg gcctggtgct
     3001 gctggcgcat cagcaaaata ttcttgtcca gatgcttggg gatctgccgg tcggatttat
     3061 ttaccgtgac tgtcagggca gcgcttttat gcctcacgcg acagactggc tcgattctat
     3121 tggcgaggcg caggcggaaa atattttcac ccatgagcag ttgctgcgct attttcctta
     3181 ttacctgctg gttaactcca cttttgctgt gaccgctgcg ctgggggctg ccgggctgga
     3241 cagcgaatcg aatctgatgg ctcgtgtacg agcatcgctg gctgaagtgc gtgatcaggt
     3301 gactcataaa acctgtctca actacgtgct ggaaagtccg tactggaacg taaaaggtaa
     3361 ctttttctgt tatctgaacg atcataacga gaacaccatt gttgaccctt cggtgatcta
     3421 tttcgatttc gctaacccgc tgcaggctca ggaggtctga atgtctgagg caaacattat
     3481 tcacagcaga tatggactgc gctgtgaaaa actcgacaag cccctgaatc ttggctgggg
     3541 actggacaat agcgcggtgt tgcactgtcc cggggagctg ccgacagggt ggctgtgcga
     3601 tgcgctggat cagatattta tcgccgcacc acaactgtca gcagtggctc tcccctgggc
     3661 cgaatggcgt gaggagccac aggcgctgac gctttttggg caggtaaaaa gcgacattat
     3721 ccatcgtact gccttctggc agttaccgtt atggttgagt tcaccggcaa accgggcctc
     3781 cggtgaaatg gtttttgatg cagagcgtga gatttatttc ccgcagcgcc caccccgtcc
     3841 acagggtgag gtttaccgcc gttacgatcc acgtattcgc aggatgctga gtttccgcat
     3901 tgccgatccc gtttctgatg cagaacgttt cacccgctgg atgaatgatc cgcgcgttga
     3961 gtatttctgg gagcaaagtg gctcactgga ggtacagacc gcctatctgg agcgccagct
     4021 caccggtaaa catgcattcc cgctgattgg gtgcttcgac gatcggccat tcagctattt
     4081 cgaaatctac tgggcggcgg aagaccgcat tggtcgtcac tattcgtggc agccctttga
     4141 ccgtggcctg catctgctgg ttggtgaaca gcaatggcgc ggggcccact atgtgcaaag
     4201 ctggctgcgc gggctgacac attacctgct gctggatgag ccccgtacgc agcgcacagt
     4261 actggagcca cgcaccgata accagcgcct gttccgccat cttgagcctg cgggataccg
     4321 gacaattaaa gagttcgact tcccacataa gcgctcgcgc atggtgatgg cggatcgtca
     4381 tcacttcttc acggaggtcg gtctgtaatg aatcacaagg actgggattt tgttaaccgt
     4441 cggctggtgg caaaaatgtt gtctgagatg gagtatgagc aggttttcca cgcggaatct
     4501 cagggcgatg accactactg cattaacctg ccgggagcac aatggcgctt catcgctgaa
     4561 cgtggtatct ggggctggct gtggattgat gcccaaacgt tgcgctgcac ggacgaacca
     4621 gtactggcgc agacgctgct gatgcagcta aagccggtgc tgtcaatgag tgatgccact
     4681 gttgcagagc atatgcagga tttgtatgcc acgctactgg gcgacctgca actgctgaag
     4741 gcccgtcgcg gactgagtgc cagtgacctg attaatcttg atgccgatcg tctgcaatgc
     4801 ctgctgagcg gtcatcctaa attcgttttt aataaaggtc gccgtggctg gggcaaagag
     4861 gcgctggaac gatatgcgcc agagtatacc aacaccttca gactgcactg gctggcggta
     4921 aaacgtgaac atatgatctg gcgctgcgat aacgatctgg atattcagca gttgttgact
     4981 gccgcaatgg atccgcagga gtttacacgc ttcagtcagg tctggcagga aaacgggctg
     5041 gatcacaact ggctaccgct gccggtacat ccgtggcagt ggcagcaaaa aatcgccaca
     5101 gacttcatcg ctgattttgt cgaaggcagg atggtgtctc tcggcgagtt tggcgaccag
     5161 tggctcgccc agcagtcgct gcgtaccctg accaacgcca gccgccgggg agggctggat
     5221 atcaagctgc cgctgaccat ctacaacacc tcatgctacc gggggatccc tggcagatat
     5281 atcgctgccg gaccactggc ttcacgctgg ctgcaacagg tttttgcgac cgacgccacc
     5341 ctggtgcaaa gcggcgcagt gatccttggt gaaccggcgg caggctatgt gtcccatgaa
     5401 ggctatgccg cgcttgcccg ggctccctat cgctaccagg aaatgcttgg tgttatctgg
     5461 cgggagaatc cgtgccgctg gctgaaaccg gatgaaagtc ctgttctgat ggcaacactg
     5521 atggagtgcg acgaaaacaa tcagccgctg gcaggcgcat atatcgaccg ctccggactg
     5581 gacgctgaaa cctggcttac gcaattgttc cgggtggttg tggttccgct gtatcacctg
     5641 ctttgccgct acggtgtcgc gcttattgca cacggacaaa atataacgct cgccatgaaa
     5701 gagggggtac cacagcgtgt tctgctgaaa gacttccagg gcgatatgcg gctggtgaaa
     5761 gaagcgtttc ccgaaatgga ctctttgcct caggaggtgc gtgatgccac atcccgtctg
     5821 agtgcggact acttaatcca tgatttgcag acgggtcact tcgtgacagt actgcgtttt
     5881 atttcgccac tgatggttcg tcttggcgta cctgaaaggc gattttatca actgctggca
     5941 gcagtgttga gtgattacat gaacaaacat ccacaaatgg cagagcgttt tgcgcttttc
     6001 tcactcttca ggccacaaat cattcgcgtg gtgctgaacc cggtaaaact gacctggccg
     6061 gacctggatg gcggcagtcg catgctgccg aattaccttg agaatctgca aaatccgctg
     6121 tggctggtaa ctcaggaata tgaatcatga aaaaaagtgt cgattttatt ggtgtcggga
     6181 cagggccatt taatctcagc attgctgcat tgtcacatca gatcgaagaa ctgaactgtc
     6241 tcttctttga cgaacatcct catttttcct ggcatccggg tatgctggta ccggattgtc
     6301 atatgcagac cgtctttctg aaagatctgg tcagtgcagt tgcacctaca aatccctaca
     6361 gctttgttaa ctatctggtg aagcacaaaa agttctatcg cttccttaca agcaggctac
     6421 gtacagtatc ccgtgaagag ttttctgact atctccgctg ggctgctgaa gatatgaata
     6481 acctgtattt cagtcatacc gttgaaaata ttgatttcga caaaaaaagt cgattgtttc
     6541 tggttcagac cagtcgggga gaatattttg cccgcaatat ctgccttggt acaggaaaac
     6601 aaccttattt accaccctgt gtgaagcatg tgacacaatc ctgtttccat gccagtgaaa
     6661 tgaatcttcg tcggccggac ctgagtggaa aacggataac cgtggttggt ggaggacaga
     6721 gtggtgcaga cctgttcctt aatgcattac gcggggaatg gggagaagcg gcggaaataa
     6781 actgggtctc acggcgtaat aattttaacg cactggatga ggctgctttt gctgatgagt
     6841 attttacacc tgaatatatt tcaggcttct ccggactgaa ggaagatatt cgccatcagt
     6901 tactggatga gcagaaaatg acatcggatg gcatcactgc cgattcttta ctgaccattt
     6961 atcgtgagtt gtaccaccgt tttgaagttc tgagaaaacc aagaaatatc cgtctgctac
     7021 ccagtcgctc ggtaataact ctggaaagta gtggtccggg ctggaggtta ttgatggaac
     7081 atcatctgga tcagggcaga gagagcctgg aaagtgatgt ggtgattttc gccacaggtt
     7141 accgttctgc attgccacaa atacttccct cactgatgcc cttgatcacc atgcacgata
     7201 agaacacctt taaagtgcgt gatgacttca ctctggaatg gagtggcccg aaagaaaata
     7261 acatctttgc ggtcaacgcc agcatgcaaa ctcatggcat cgccgaaccc cagctcagcc
     7321 tgatggcctg gcgatctgca cgtattctta atcgcgtact ggggcgtgat ttattcgatc
     7381 tcagtatgcc gcccgcactg attcagtggc gcagcggcag ccggaaaaaa ccgcagccgg
     7441 aggctgcttc tttaactcac tatacagcaa atattcagga ataacgatga tgcgcaaaaa
     7501 gtatatgccc cgggctcttg gtccgctgct tcttgtcgtg ctgtcaccag ctgtcgccca
     7561 gcaaaacgat gataatgaga tcatagtgtc tgccagccgc agcaatcgaa ctgtagcgga
     7621 gatggcgcaa accacttgga ttatcgaaaa tgccgaactg gagcagcaga ttcagggcgg
     7681 taaagagctg aaagacgcac tggctcagtt aatccccggc cttgatgtca gcagccagag
     7741 tcgaaccaac tacggtatga acatgcgtgg ccgcccgctg gttgtcctga ttgacggtgt
     7801 gcgcctcaac tcttcacgtt ccgacagccg acaactggac tctgtcgatc cttttaatat
     7861 cgaccatatt gaagtgatct ccggcgcgac ggccctgtac ggtggcggga gtaccggagg
     7921 gttgatcaac atcgtgacca aaaaaggtca gccggaaacc atgatggagt ttgaggctgg
     7981 cacaaaaagt ggctttaaca gcagtaaaga tcacgatgag cgcattgccg gtgctgtctc
     8041 cggcggaaat gaccatatct ccggacgtct ttccgtggca tatcagaaat ttggcggctg
     8101 gtttgacggt aacggcgatg ccaccctgct tgataacacc cagaccggcc tgcagcactc
     8161 caatcggctg gacatcatgg gaaccggtac gctgaacatc gatgaatccc ggcagcttca
     8221 actgataacg cagtactata aaagtcaggg agacgacaat tacgggctta atctcgggaa
     8281 aggcttttcc gccatcagcg ggagcagcac accatacgtc agtaaggggc tgaattctga
     8341 ccgcattccc ggcactgagc ggcatttgat cagcctgcag tactctgaca gtgatttcct
     8401 gggacaggaa ctggtcggtc aggtttacta ccgcgatgag tcgttgcggt actacccgtt
     8461 cccgacggta aatgcgaata aacaggcgac ggctttctcc tcgtcacagc aggataccga
     8521 ccagtacggc atgaaactga ctctgaacag ccaactgatg gacggctggc aaatcacctg
     8581 ggggctggat gctgagcatg agcgctttac ctccaaccag atgttcttcg atctggctca
     8641 ggcaagtgct tccggagggc tgaacaacca taagatttac accaccgggc gctatccgtc
     8701 atatgacatc accaatctgg cggccttcct gcaatccagc tatgacatta atgatatttt
     8761 taccgttagc ggtggcgtac gctatcagta tactgagaac agggtagatg atttcatcga
     8821 ctacacgcag caacagaaga ttgctgccgg gaaggcgata tctgccgacg ccattcctgg
     8881 tggttcggta gattacgata actttctgtt caatgctggt ctgctgatgc acatcaccga
     8941 acgtcagcag gcatggttca atttttccca gggggtggca ttgccggatc cggggaaata
     9001 ttatggtcgc ggcatctatg gtgcagcagt gaacggccat ctttccctga caaagagcgt
     9061 gaacgtcagc gacagtaagc tggaaggcgt gaaagtcgat tcttatgaac tgggctggcg
     9121 ctttaccggt gacaacctgc ggactcaaat cgcggcatat tactcgcttt ccaataagag
     9181 cgtggaaagg aataaagatc tgaccatcag tgtgaaggac gacaggcgcc gtatttacgg
     9241 cgtggaaggt gcggtggact acctgatccc ggatactgac tggagtaccg gtgtgaactt
     9301 caatgtgctg aaaaccgagt cgaaagtgaa cggtcaatgg caaaaatatg acgtgaagga
     9361 atcaagtcca tcgaaagcga cagcttacat taactgggcg ccggaaccgt ggagtctgcg
     9421 tgtacagagc accacttctt tcgacgtaag cgatgcagag ggtaacgata ttaatggtta
     9481 cactaccgtc gattttatca gtagttggca gcttccggtg ggaacactca gcttcagcgt
     9541 tgagaacctc ttcgaccgtg actataccac tgtctgggga cagcgtgcac ctctgtacta
     9601 cagcccgggt tacggccctg cttcactgta cgactacaaa ggccggggcc gaacctttgg
     9661 tctgaactac tcagtgctgt tctgacgggg atgcttttgc aacaagggta tgctgtaaac
     9721 aacatggtcc ttggtggcca tgttgtttac agccagtata atatacagcc ttaacaagat
     9781 attccgtctc cggaatggac tgttcaccac tgaataacat tatttctgtt ttatggatct
     9841 ttgactgaaa tttatcgcat atcctcattt cattgtccgt ctgaaaacta accttacaag
     9901 taagcgtcgt ctcagcaccg tctggcagat cctgaaattc ctgagaggat gatctg
//
LOCUS       DAJTMC010000155         9894 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.155, whole genome shotgun sequence.
ACCESSION   DAJTMC010000155 DAJTMC010000000
VERSION     DAJTMC010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9894
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.155"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(55..1338)
                     /gene="aroA"
                     /locus_tag="OO130_003384"
     CDS             complement(55..1338)
                     /gene="aroA"
                     /locus_tag="OO130_003384"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HCR5366661.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            complement(1409..2497)
                     /gene="serC"
                     /locus_tag="OO130_003385"
     CDS             complement(1409..2497)
                     /gene="serC"
                     /locus_tag="OO130_003385"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HCR5366662.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSIFNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(2696..3387)
                     /locus_tag="OO130_003386"
                     /pseudo
     CDS             complement(2696..3387)
                     /locus_tag="OO130_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
     gene            3517..5277
                     /locus_tag="OO130_003387"
     CDS             3517..5277
                     /locus_tag="OO130_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HCR5366663.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            5683..6540
                     /gene="focA"
                     /locus_tag="OO130_003388"
     CDS             5683..6540
                     /gene="focA"
                     /locus_tag="OO130_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HCR5366664.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            6595..8877
                     /gene="pflB"
                     /locus_tag="OO130_003389"
     CDS             6595..8877
                     /gene="pflB"
                     /locus_tag="OO130_003389"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCR5366665.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKQLEKIVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKLAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            9069..9809
                     /gene="pflA"
                     /locus_tag="OO130_003390"
     CDS             9069..9809
                     /gene="pflA"
                     /locus_tag="OO130_003390"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HCR5366666.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
BASE COUNT         2534 a         2488 c         2355 g         2517 t
ORIGIN      
        1 atgtgccgac gttttgataa agaaagattt ggctatttat tgcccgttgt tcattcaggc
       61 tgcctggcta atccgcgcca actgctcgaa ataatccgga aatgttttgg ccgtacattt
      121 gggatcaaga atcgtcactg gtgtatctga caacgccacc agcgagaaac acatcgccat
      181 ccggtgatca ttgtatgtcg cgatctcggc aaagttcagt ttttccggtg gagtgatacg
      241 aatgtaatcg tgcccctctt ctacttccgc accgacttta cgcagttctg ttgccatcgc
      301 aaacaggcga tcggtttctt taacacgcca gttatagata ttgcgcagcg tggtggtgcc
      361 ttttgcaaat aacgccgccg tggcaatggt catcgccgca tcgggaatat ggttcatatc
      421 catatcaata gcgttcagtt caccacgcgt gcaggaaata taatcatcgc cccagcaaat
      481 ggtcgcgccc attttttcca gcacatcagc aaagcgaata tcaccctgca tactgttacg
      541 tccaataccg gtcactttta cagtgccgcc tttgattgct gctgctgcca gaaagtaaga
      601 agccgaagat gcatcgcctt cgaccaaata agtacccgga gactgataag actgtccgcc
      661 ttttacgaca aattgttgat agtgctgatt ttcaatttca acaccaaacg tcttcatcag
      721 attgagtgtg atgtcgatat aaggtttaga aaccagatcg cctttaatac gaatcaccgt
      781 atcttccggc gcaagaggcg cagtcattaa cagtgcggtg aggaattggc tggaaacgga
      841 gccatcaacg tcaacgttgc cgccagtaaa gccgccctgt aaacgcaacg gcggataatt
      901 ttcttgttcc aggtaagtga tcttcgcccc gcccaggcgc agcgcatcca ccagatgacc
      961 aatcgggcgt tctttcatac gcggctcacc ggtcagcaca atatcattgc tacccagaca
     1021 aagagctgcc gccagcggac gcattgccgt tccggcgtta ccgaggaaca actccagggc
     1081 accttctgcg tgtaatggac cgccgttacc gataatttcg caacgcgtac gatcggctga
     1141 aagcgtatag cttaccccta acgctgttaa tgcattcagc atatggcgca cgtcatcgct
     1201 atccagcaga ttggttaata ctgttttgcc gtgtgctaat gccgccagca ataaagcgcg
     1261 gttagaaacg ctcttggaac cgggcagatt aatagtgcca tcgacacgag cgatgggttg
     1321 taacgtcagg gattccatga actcaactct ctacaacaga aataaaaacc ccacaggctg
     1381 gctgtgggga ttaagcaaaa tttcggcatt aaccgtgacg gcgttcgaac tcaaccatga
     1441 agtctgtcag cgctttaacg ccttccagcg gcatggcgtt ataaatagaa gcgcgcattc
     1501 cgccgaccac acggtgacct ttcagtgcat gaaggccagc agcaaaagac tcttcaagga
     1561 acaatttgtc aagcgcactg tccgccaact ggaacggcac gttcatccgc gaacggttag
     1621 ctttcgccac gtcattgcgg tagaaatcgc tgttatcaat caccccatat agcagttctg
     1681 ctttttgctg attgatttta tccatttcag ctacaccgcc gttcgctttc agccatttaa
     1741 agaccagacc agatagatac caggcaaatg tcggcggcgt gttaaacatg gagccgttat
     1801 cgttgaagat ggaataatcc agtatcgacg gacacgcgat attcgctttg cccagcaaat
     1861 cttcacgaac gatgacgatt gtcaggccag ccgggccgat atttttctgc gcgccagcgt
     1921 aaatcacgcc ataacggctg acgtcaatcg gacgggaaag aatggttgaa gagaagtcgg
     1981 cggcgaccac cacatctttg ccgaagtctg gcgtctcgtc gatggcgata ccgtcgatgg
     2041 tttcattcgg gcagtaatgc atataagcag cattatcaga gagttgccat tcacgcattg
     2101 gcttaaccgc gcgcagacca tcaacagtca ctttggcgtc aaagacatta ggcgtgcagt
     2161 attttttcgc ttctttaatg gcacttgccg cccagtaacc ggcatcaaca taatctgcgg
     2221 tggttttatc accgagaata ttcagcggta ccgcagcaaa ctgaccgcga ccaccgccat
     2281 ggcagaataa taccttgtag ttggagggga cattaagaag atcgcgaaaa tccttctcgg
     2341 cttcctctgc aacctgaatg aactctttgc cacggtgact cacttccatc accgacgtac
     2401 caagaccgtt ccagtcgcgc agttcctgtt gagcctgttt aagcacctct gccggtagca
     2461 ttgccggacc agaactaaaa ttgaagattt gagccatttc ccctcaccac gttgcgttgc
     2521 tattaacccg cggtcaatcg attgcgacca caggtaatac attattcctg tggctatcgg
     2581 ttttatcatt cagtgacaca catcgcaatg agtaaaaccg ttgagggcaa aaatgaggct
     2641 tgcatcacac aaaagaatct ctcggattat tagcaaaaag ccgatgtttt tactgtcagg
     2701 tcacccgttt tgcccgactt gccttcgtcc attctggatt tgcacagcgc ggacataatt
     2761 gttttccccc gctttcatat gaatgatttc actacaacga atacaggcat agtccgccga
     2821 ctccggcacc actttgtaac gctgcgtaca atcactgggt aaaataagta aacccggttt
     2881 caccttgtca tcttcaaaga aatagacact gatttgcccg ttggtttcga gaatcgccag
     2941 acgtacctgc cccagctgct ccacgccacg caatcgtagc tccataaaga actcaaattc
     3001 cgtcatgttg gagttattga gtttcgacca ggccagctcg ccatcttcaa taataacaac
     3061 cggcttacct tccagtagat cttccagttt ttcactgtgc gccatcaacc acattaccag
     3121 gcggtataac aacgccagag tgataaagac gataagtacc ggcaccatcg gcacatcatc
     3181 ataaaacgcc acatctcccg ccgctgaacc cagcgtgaga atgattaaca cttcaaacag
     3241 cgacatctgt cgcacaccgc gtcttccggt cattttgagg aacaaaaaaa ccagtacaaa
     3301 ggtataaaga ctacgtagtg caacttcgcc aaggaaatca aaaggcactt tatcaaatgc
     3361 catacggtgg agatcgaacg ccttcatttt tatgctctta tcgtggttta ttcgctaaaa
     3421 gcatagtcaa cagttcatcg gcatggcggc ggtgatagcg cctttgagat aaaacccgta
     3481 ttattgcgcg ctttccgtac gactaaagtg attttcatga cgcaaacatt tatccccggc
     3541 aaagatgccg ctctggaaga ttccatcgct cgcttccagc aaaaactttc agacctcggc
     3601 tttcagattg aagaggcctc ctggctgaat cctgtgccta acgtctggtc tgtacatatt
     3661 cgcgacaaag agtgcgcact gtgttttacc aacggtaaag gcgcaaccaa aaaagcggcg
     3721 ctggcttctg cacttggtga atatttcgag cgtctctcaa ccaactactt ttttgctgat
     3781 ttctggctgg gcgaaaccat cgccaacggt ccgttcgtgc attatcccaa cgaaaaatgg
     3841 ttcccactga ccgaaaatga cgatgtgcca gaaggactac tcgatgaccg tctgcgcgcg
     3901 ttttatgatc cggagaatga actgaccggc agcatgctga ttgacctaca atccggtaac
     3961 gaagatcgtg gtatttgcgg cctgccgttt acgcgtcagt ccgacaatca gaccgtttat
     4021 attccgatga atatcattgg taacctgtac gtctccaacg gtatgtccgc aggtaatacc
     4081 cgcaacgaag cacgcgttca gggattgtct gaagttttcg aacgctacgt gaaaaaccgc
     4141 attattgctg aaagcatcag cctgccagag atcccggcag acgtgctggc gcgttaccca
     4201 gcggtggttg aagcgatcga aacactggaa gcagaaggtt tcccgatctt cgcatatgat
     4261 ggttcccttg gcggccagta tccggtgatt tgcgtggtac tgttcaatcc tgctaacggt
     4321 acctgctttg cctctttcgg tgcgcatcct gattttggcg tagcactgga acgtaccgtg
     4381 accgagctgc tgcaaggtcg tggcctgaaa gatttggatg tgtttactcc gccaaccttc
     4441 gatgatgaag aagtcgctga acataccaac ctcgaaacgc actttatcga ttccagcggt
     4501 ttaatctcct gggacctgtt caagcaggat gccgattatc cgtttgtgga ctggaatttc
     4561 tccggcacca cggaagaaga gtttgctacg ctgatggcta tcttcaacaa agaagataaa
     4621 gaagtttata ttgccgatta cgagcatctg ggcgtttatg cttgccgtat tatcgtgcct
     4681 ggcatgtccg atatttatcc ggctgaagat ctgtggctcg cgaataacag tatgggcagc
     4741 catttacgtg aaacgattct ttcgctacca ggcagcgagt gggaaaaaga agattacctg
     4801 aacctcatcg agcaactgga tgaagaaggt tttgatgact ttacccgcgt gcgtgagctg
     4861 ttgggtctgg cgaccgggtc ggataacggt tggtacaccc tgcgtatcgg tgaattaaaa
     4921 gccatgctgg cgctggctgg tggcgatctg gaacaggctc tggtctggac cgaatggacg
     4981 atggagttta actcatcagt atttagtccg gaacgcgcca actattatcg ctgcctgcaa
     5041 acgttgttat tactggcaca ggaagaagat cgccagccgc tgcaatatct gaatgcgttt
     5101 gttcgcatgt acggcgcaga tgccgtggaa gccgccagtg cggcaatgag cggcgaagcg
     5161 gcgttttacg gcctgcaacc ggtagatagc gatctgcacg cgtttgctgc acatcagtcg
     5221 ttgttgaagg cctacgaaaa gctgcagcgc gccaaagcag cattctgggc aaaataaaat
     5281 caaatagcct acgcaatgta ggcttaatga ttagtctgag ttatattacg gggcgttttt
     5341 ttaatgcccc gctttacata tatttgcatt aataaaataa ttgtaattat aaggttaaat
     5401 atcggtaatt tgtatttaat aaatacgatc gatattgtta ctttattcgc ctaatgctcc
     5461 cttttaatta actattttag cggaggatgc ggaaaaaatt caactcattt gttaattttt
     5521 aaaatttatt tttatttgga taatcaaata tttactccgt atttgcataa aaaccatgcg
     5581 agttacggac ctataagcca ggcgagatat gatctatatc aatttctcat ctataatgct
     5641 ttgttagtat ctcgtcgccg acttaataaa gagagagtta gtgtgaaagc tgacaaccct
     5701 tttgatcttt tacttcctgc tgcaatggcc aaagtggccg aagaggcggg tgtctataaa
     5761 gcaacgaaac atccgcttaa gactttctat ctggcgatta ccgccggtgt tttcatctca
     5821 atcgcattcg tcttctatat cacagcaacc actggcacag gcacaatgcc cttcggcatg
     5881 gcaaaactgg ttggcggcat ttgcttctct ctggggctga ttctttgtgt tgtctgcgga
     5941 gctgatctct ttacttccac cgtgttgatt gttgttgcta aggcgagtgg gcgcatcacc
     6001 tggggtcagt tggcgaaaaa ctggctaaat gtctattttg gcaacctggt cggcgcactg
     6061 ctgtttgtac ttttaatgtg gctttccggc gagtatatga ccgcaaatgg tcaatgggga
     6121 ctaaacgtcc tacaaaccgc cgaccacaaa gtgcaccata cttttattga ggccgtctgt
     6181 cttggtatcc tggcaaacct gatggtatgt ctggcagtat ggatgagtta ttctggccgc
     6241 agcctgatgg acaaagcgtt cattatggtg ctgccggtcg cgatgtttgt tgccagcggt
     6301 tttgagcaca gtatcgcaaa catgtttatg atcccgatgg gtattgtaat ccgcgacttc
     6361 gcatctccgg aattctggac cgctgtcggt tctgcaccgg aaaatttttc tcacctgacc
     6421 gtgatgaact tcatcactga taacctgatc ccggttacga tcggtaatat tatcggcggt
     6481 ggtttgttgg ttgggttgac atactgggtc atttacctgc gtgaaaacga tcaccattaa
     6541 tggttgtcga agtacgcagt aaataaaaaa tccacttaag aaggtaggtg ttacatgtcc
     6601 gagcttaatg aaaagttagc cacagcctgg gaaggtttta ccaaaggtga ctggcagaat
     6661 gaagtaaacg tccgtgactt cattcagaaa aactacactc cgtacgaggg tgacgagtcc
     6721 ttcctagctg gcgctactga agcgaccacc accctgtggg acaaagtaat ggaaggcgtt
     6781 aaactggaaa accgcactca cgcgccagtt gactttgaca ccgctgttgc ttccaccatc
     6841 acctctcacg acgctggcta catcaacaag cagcttgaga aaatcgttgg tctgcagact
     6901 gaagctccgc tgaaacgtgc tcttatcccg ttcggtggta tcaaaatgat cgaaggttcc
     6961 tgcaaagcgt acaaccgcga actggatccg atgatcaaaa aaatcttcac tgaataccgt
     7021 aaaactcaca accagggcgt gttcgacgtt tacactccgg acatcctgcg ttgccgtaaa
     7081 tctggtgttc tgaccggtct gccagatgca tatggccgtg gccgtatcat cggtgactac
     7141 cgtcgcgttg cgctgtacgg tatcgactac ctgatgaaag acaaactggc acagttcact
     7201 tctctgcagg ctgatctgga aaacggcgta aacctggaac agactatccg tctgcgcgaa
     7261 gaaatcgctg aacagcaccg cgctctgggt cagatgaaag aaatggctgc gaaatacggc
     7321 tacgacatct ctggtccggc taccaacgct caggaagcta tccagtggac ttacttcggc
     7381 tacctggctg ctgttaagtc tcagaacggt gctgcaatgt ccttcggtcg tacctccacc
     7441 ttcctggatg tgtacatcga acgtgacctg aaagctggca agatcaccga acaagaagcg
     7501 caggaaatgg ttgaccacct ggtcatgaaa ctgcgtatgg ttcgcttcct gcgtactccg
     7561 gaatacgatg aactgttctc tggcgacccg atctgggcaa ccgaatctat cggtggtatg
     7621 ggcctcgacg gtcgtaccct ggttaccaaa aacagcttcc gtttcctgaa caccctgtac
     7681 accatgggtc cgtctccgga accgaacatg accattctgt ggtctgaaaa actgccgctg
     7741 aacttcaaga aattcgccgc taaagtgtcc atcgacacct cttctctgca gtatgagaac
     7801 gatgacctga tgcgtccgga cttcaacaac gatgactatg ctatcgcttg ctgcgtaagc
     7861 ccgatgatcg ttggtaaaca aatgcagttc ttcggtgcgc gtgcaaacct ggcgaaaacc
     7921 atgctgtacg caatcaacgg cggcgttgac gaaaaactga aaatgcaggt tggtccgaag
     7981 tctgaaccga tcaaaggcga tctcctgaac tacgatgaag tgatggagcg catggatcac
     8041 ttcatggact ggctggctaa acagtacatc actgcactga acatcatcca ctacatgcac
     8101 gacaagtaca gctacgaagc ctctctgatg gcgctgcacg accgtgacgt tatccgcacc
     8161 atggcgtgtg gtatcgctgg tctgtccgtt gctgctgact ccctgtctgc aatcaaatat
     8221 gcgaaagtta aaccgattcg tgacgaagac ggtctggcta tcgacttcga aatcgaaggc
     8281 gaatacccgc agtttggtaa caatgatccg cgtgtagatg acctggctgt tgacctggta
     8341 gaacgtttca tgaagaaaat tcagaaactg cacacctacc gtgacgctat cccgactcag
     8401 tctgttctga ccatcacttc taacgttgtg tatggtaaga aaactggtaa caccccagac
     8461 ggtcgtcgtg ctggcgcgcc gttcggaccg ggtgctaacc cgatgcacgg tcgtgaccag
     8521 aaaggtgctg tagcgtctct gacttccgtt gctaaactgc cgtttgctta cgctaaagat
     8581 ggtatctcct acaccttctc tatcgttccg aacgcactgg gtaaagacga cgaagttcgt
     8641 aagaccaacc tggctggtct gatggatggt tacttccacc acgaagcatc catcgaaggt
     8701 ggtcagcacc tgaacgttaa cgtgatgaac cgtgaaatgc tgctcgacgc gatggaaaac
     8761 ccggaaaaat atccgcagct gaccatccgt gtatctggct acgcagtacg tttcaactcg
     8821 ctgactaaag aacagcagca ggacgttatt actcgtacct tcactcaatc tatgtaatta
     8881 gatttgactg aaatcgtaca gtaaaaagcg tacaataaag gctccacgaa agtggggcct
     8941 tttttagcac gagagccttt tttgtcagct atctatactt taaggtgact gccaaaacag
     9001 actcgacgta gccttcgagc tgcgcaccaa cacggcctca gatgggccac atctggagaa
     9061 ataccgcaat gtcagttatt ggtcgcattc actcctttga atcctgtgga accgtagacg
     9121 gcccgggtat tcgctttatc acctttttcc agggctgcct gatgcgctgc ctgtattgtc
     9181 ataaccgcga cacctgggac acgcatggcg gtaaagaagt taccgttgaa gatttgatga
     9241 aggaagtggt gacctatcgc cactttatga acgcttccgg cggcggcgtt accgcatccg
     9301 gcggtgaagc aatcctgcaa gctgagtttg ttcgtgactg gttccgcgcc tgcaaaaaag
     9361 aaggcattca tacctgtctg gacaccaacg gttttgttcg tcgttacgat ccggtgattg
     9421 atgaactgct ggaagtaacc gacctggtaa tgctcgatct caaacagatg aacgacgaga
     9481 tccaccaaaa tctggttgga gtttccaacc accgcacgct ggagttcgcc aaatatctgg
     9541 cgaacaaaaa tgtgaaggtg tggatccgct acgttgttgt cccaggctgg tctgacgatg
     9601 acgattcagc gcatcgcctc ggtgaattta cccgtgatat gggcaacgtt gagaaaatcg
     9661 aactcctccc ctaccacgag ctgggcaaac acaaatgggt ggcaatgggt gaagagtaca
     9721 aactcgacgg tgttaaacca cctaagaaag agaccatgga acgcgtgaaa ggcattcttg
     9781 agcagtacgg tcataaggta atgttctaat ttccccgtaa agcggcaact ttattgagtt
     9841 gccgcttttt tattccgcgc ttcattctgc tacagtgttt ttaacacatt ttat
//
LOCUS       DAJTMC010000156         9795 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.156, whole genome shotgun sequence.
ACCESSION   DAJTMC010000156 DAJTMC010000000
VERSION     DAJTMC010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9795
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.156"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            124..744
                     /gene="fklB"
                     /locus_tag="OO130_003391"
     CDS             124..744
                     /gene="fklB"
                     /locus_tag="OO130_003391"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR5366667.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            1053..2465
                     /gene="cycA"
                     /locus_tag="OO130_003392"
                     /gene_synonym="dagA"
     CDS             1053..2465
                     /gene="cycA"
                     /locus_tag="OO130_003392"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HCR5366668.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFLG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(2510..3172)
                     /gene="ytfE"
                     /locus_tag="OO130_003393"
     CDS             complement(2510..3172)
                     /gene="ytfE"
                     /locus_tag="OO130_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HCR5366669.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYNMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(3280..4245)
                     /locus_tag="OO130_003394"
     CDS             complement(3280..4245)
                     /locus_tag="OO130_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR5366670.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALVCIGIGLACVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNTQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLIRQQMPPLMTLSGIALLVVGVVIVVRAKPEKPLTESVSES"
     gene            complement(4353..5213)
                     /gene="qorB"
                     /locus_tag="OO130_003395"
     CDS             complement(4353..5213)
                     /gene="qorB"
                     /locus_tag="OO130_003395"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HCR5366671.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLTDSDVGASKGGLFDNSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            5302..5682
                     /locus_tag="OO130_003396"
     CDS             5302..5682
                     /locus_tag="OO130_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCR5366672.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            complement(5811..7754)
                     /gene="cpdB"
                     /locus_tag="OO130_003397"
     CDS             complement(5811..7754)
                     /gene="cpdB"
                     /locus_tag="OO130_003397"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HCR5366673.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIIGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEEADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDRHIAFASPDE
                     NRSVLTAWIADESKRAGEIHPAADNNWRLAPIAGDKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            7944..8684
                     /gene="cysQ"
                     /locus_tag="OO130_003398"
     CDS             7944..8684
                     /gene="cysQ"
                     /locus_tag="OO130_003398"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HCR5366674.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            8896..>9795
                     /locus_tag="OO130_003399"
     CDS             8896..>9795
                     /locus_tag="OO130_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5366675.1"
                     /translation="MLPRIRHNNFIGAVELFVKSSHTKTHSNNFFNNIQHAFKKKDWV
                     SNYDSLLTLWEFFRCATQIDKDSYQVLSSKNETVKAMDKFLISFSLKDNGAEYTMTLR
                     GSGFEYEEIPITINEYNSFMDFKNREFPLEQNRRLYAWDILQKKQSDIPKRIKGYIHQ
                     AIGDVSLGYALLEDIVSKLKRGKFELQIPGGGIKECDGWYIYEKIIDDNFAIVIESLG
                     FALKIYGGDERFRNGSSVVLEDEDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAVDS
                     DITEEEICEKYKSTIEAFKKEQLA"
BASE COUNT         2392 a         2360 c         2505 g         2538 t
ORIGIN      
        1 acgggatatt gctattgttt ctttttcccc gggatttgtc atcattcctg tgacaaagtt
       61 ttacccaaag aagtgtggct gatatgctgc ccgctacttt tataccctaa gaaaggaaat
      121 acgatgacca ccccaacttt tgacactatc gaagcgcaag caagctacgg cattggtttg
      181 caggtagggc aacaactgag tgaatctggc ctggaagggc tgctgccaga agcactggtt
      241 gcaggtattg ccgatgcgct ggaaggcaaa catccggctg ttccggttga tgtggtgcat
      301 cgcgcgctgc gtgaaatcca cgaacgcgcc gatgccgttc gtcgtcagcg tttccaggcg
      361 atggctgctg aaggtgtgaa atacctggaa gaaaacgcca aaaaagaagg tgtgaatagc
      421 accgaatctg gcctgcaatt ccgcgtgatc aaccagggtg aaggcgcaat tccggcacgt
      481 accgaccgcg ttcgtgttca ttacaccggt aaactgatcg acggcaccgt gtttgacagc
      541 tccgttgctc gtggtgaacc cgctgaattc ccggttaacg gcgtgatccc tggctggatt
      601 gaagcactga ctctgatgcc ggtaggttct aaatgggaac tgactatccc gcaggaactg
      661 gcatatggcg agcgcggcgc aggtgcatcc atccctccgt tcagcaccct ggtgtttgaa
      721 gtcgaactgc tggaaatcct ctaagcagcg cattctgttc ccctcgaacc agaggggagc
      781 aggcattcag caataaaccc ttcagtttgc caaacggcgc tattttgtgt tgcaaagacc
      841 ccgtaagcgt gtatttttgt gagatgtttc gcgttatcac cgtgatatga cactcacttt
      901 aaacataaaa ttaacattag atctaaatct tagtattcat cccgcgtatt gttacctaat
      961 atcgatgagt cccgatacag attcgtcgta tcatagactg actaaaggcc gtagagcctg
     1021 aacaacacag acaggtacag gaagaaaaaa acatggtaga tcaggtaaaa gtcgttgccg
     1081 atgatcaggc tccggctgaa cagtcgctac ggcgcaatct cacaaaccga catattcagc
     1141 ttattgccat tggcggtgcc attggtacgg ggttgtttct ggggtctggc aaaacgatta
     1201 gccttgccgg gccgtcgatc attttcgttt atatgatcat tggttttatg ctctttttcg
     1261 tgatgcgggc aatgggggaa ttgctgcttt cgaatctgga atacaaatct tttagtgact
     1321 tcgcttccga tttactcggg ccgtgggcag gatatttcac tggctggact tactggttct
     1381 gctgggttgt aaccggtatg gcagacgtgg tggcgatcac ggcttatgct cagttctggt
     1441 tccccgatct ctccgactgg gtcgcctcgc tggcggtgat agtgctgctg ctgacgctca
     1501 accttgccac cgtgaaaatg ttcggtgaga tggagttctg gtttgcgatg atcaaaatcg
     1561 tcgccatcgt gtcgctgatt gtcgtcggcc tggtcatggt ggcgatgcac tttcagtcac
     1621 cgactggtgt ggaagcgtca ttcgcgcatt tgtggaatga cggcggctgg ttcccgaaag
     1681 gtttaagtgg cttctttgcc ggattccaga tagcggtttt cgctttcgtg gggattgagc
     1741 tggtaggtac aacagctgcg gaaaccaaag atccggagaa atcactgcca cgcgcgatta
     1801 actccattcc gatccgtatc attatgttct acgtcttcgc gctgattgtg attatgtccg
     1861 tgacaccgtg gagttcggta gtcccggaga aaagcccgtt cgttgagctg tttgtgttgg
     1921 taggtttgcc tgcggctgcc agcgtgatca actttgtggt gctgacctct gcggcgtctt
     1981 ccgctaacag cggcgtcttc tctaccagcc gtatgctgtt tggtctggcg caggaaggtg
     2041 tggcaccgaa agcgttcgct aaactttcta agcgcgcagt accagcgaaa gggctgacct
     2101 tctcctgcat ctgcctgctc ggtggcgtgg tgatgctgta tgtgaatcct agcgtgattg
     2161 gtgcgttcac gatgattaca accgtttccg cgattctgtt tatgttcgtc tggacgatta
     2221 tcctttgctc gtacctggtg taccgcaaac agcgtcctca tctgcatgag aagtcgatct
     2281 acaagatgcc gctcggcaag ctgatgtgct gggtatgtat ggcgttcttt gtgttcgtgg
     2341 tcgtgttgct gacactggaa gatgacactc gccaggcgct gctggttacc ccgctgtggt
     2401 ttatcgcact ggggctgggc tggttgttta ttggtaagaa gcgggctgct gaactgcgga
     2461 aataaccgca ttatcatgct ggatggcgca atgccatcca gcttttagat cactcacccg
     2521 ccagcgcgcg tgggaacagt acattgtttt ccagactgat gtgatccatc aggtcatcaa
     2581 tcagttcatt aatgccgtta tacatcgctt tccaggtggt gcaggcttct ggcggcggtg
     2641 tgacgttatt ggtagtgtgt ttgatcactt ccagcagttc gcccgcttca tcgtgctcgc
     2701 tttccattac gctgattggc cccattgcct ggctgcccat gccttgtttg atcatcggga
     2761 agaggatctg ctcttctttc atcatgtggc tggaaagctc ttcatgcagc atggtcaggt
     2821 attttgtcag cccttttggc acgctcggtt tgtcggcgtg aacgcgctcg actttagtcg
     2881 cttgcaggat cagctccggc agttgctcgc ggtgacgatc gtggtagcgc acgatgatat
     2941 ggtcaatgat ttctgccagc ggggcgctac gccagtcttt ctcaatcggt tgttcagcaa
     3001 gctttgccag ttcagcttca atgacctcaa catccagttc tttacgtgcc gccgcgcgcg
     3061 ccagcgtctg cttaccgcca cagcagtaat ccatattata tttacgaaac agagctgaag
     3121 cgcgaggaat agagagcgcc agttcaccta aaggttggtc gcgataagcc atagctgata
     3181 cctcattctt aataagataa gatgtatttt aaatacatct ttaaggcaaa agctataacc
     3241 cttacgtagc agaggcttat ttaactcaca gccagtatgt caactttctg agacagattc
     3301 agttaaaggc ttttccggtt ttgctctgac tacaatgacc acgccaacca ctaacagcgc
     3361 gataccgctc agcgtcatca gcggcggcat ttgctggcgt atcaaaaagg tgtacagcaa
     3421 acctgccagt gtttcgaaaa cgatcagcgg cccgagaatc actgtcggta atcgctggct
     3481 ggcgacgttc cagcagagtg cgccaaccca tgagcaaagc acggctatcg caaccatcag
     3541 actaataaac accagcggac gggggccaaa aggtagggag aagtccggcg tttgcgtatt
     3601 cagccagtaa caggcgacga gatagccgat gagagaaacc ggcagtgtga ccagcgcctg
     3661 cgccgtcgcc cacatcatcg gatgtttgtc gggattctcc cgcagccagc gggcgttgcg
     3721 cagggcatac catgcccagc agaccacgga aactaacgct agcacgatgc ctgaggtata
     3781 acgtgcccag tcaaaatcgg ggagtccgtg gtttaactca gcaatattca cacacgccag
     3841 gccgatgcca atacaaacca gtgccggggc gagttttccc cacgcgagtt tgccgtcgcg
     3901 ctggctataa agcagattgg caaaaacggg aatcaccacc ggcagggtgc cgataatcat
     3961 cgtggaaaca ggcgcaccag tacgttgaat ggcactggca aggcagaagt aatagatgag
     4021 gttgcccatc atagtgagca tcaaggcggt aagccagtcg cgacgcgcca actgacgcag
     4081 acgcacgcgt cccagccagg caatgggcag cgcaattaac cctaacgcca gataacgccc
     4141 catcgactgc aacatcgccg ggtattccgg tacgatcaac ggcccgacaa aaataagccc
     4201 ccacatcaac cctgctaaca gggcgtacag cacgccgcta atcattactg gcatccattg
     4261 gtctgtcaga agaaagttca ggaagcagac atgattgctc ccggcatccc gtcattatta
     4321 gagaggatgc gggaggatgc cactttaatt aactagttat taacattaaa aagatggctt
     4381 acgctttcgg ctaacgttgt cgttgggcgg ccaatcaatt tgctaagcgt tttgctgtta
     4441 tcaaacagac cacctttcga tgcgccaacg tcagaatccg tcagcatatc cgccagtccg
     4501 tcgggcagtc cgacgctttt cagtgccgcg gcgaaatcgg cttcgctcag attttgatag
     4561 gtaacctgtt tgccgctctg tttggtcagc tctgccgcta actgtgtcaa cgtccaggca
     4621 ctatcgcccg ccagttcgta aaccttgcct tcgtgaccgg cttcgctaat cacgcgtgcc
     4681 gcagctgccg cataatctgc ccgcgttgct gaggcgattt tgccatcgcc cgccgcaccg
     4741 ataaatacgc cgtgttccag tgctgccggg gcgctggcga ggtagttttc gctgtaccag
     4801 ccgttgcgca gcagggtgta aacgatgcca gaatcagcca acattttctc cgtctcaatg
     4861 tgctcatcgg cgaggccgag cggggaggta tctgcatgta gcaggctggt ataagcgata
     4921 aatttcacgc cagccgcctt tgcggcatta ataacattac gatgctgcgg ggcacgttga
     4981 cccacttcgc tggaagagat cagcagtagt ttttccactc cctgaagtgc agatgtcagt
     5041 gcggcttcat cgccgtagtc agcctgacgc acggtaatgc cttgtgctgc cagagcctgg
     5101 gcttttgccg gattacgaac gatagccact atttggctgg caggaaccgt tttcatcaag
     5161 gattcaataa tatagtgacc aagttggcca gtggcaccag taatagcgat catgggaagt
     5221 ctccatcgtt ttgtcttgtg tttaaatacg ctaacaccta aacttacttt tagtaagtac
     5281 gtacaaaaag gtaagtatga aatgagtcag gttagcctgt cgcaacaact gaaagagggt
     5341 aatctctttg cggaacagtg cccgtcgcgc gaggtgttga aacacgtcac cagccgttgg
     5401 ggggtgttga ttctggtggc gctacgcgaa ggtactcatc gctttagcga cctgcggcgc
     5461 aaaatcggcg gggtgagcga aaagatgctt gcgcagtcgt tacaggcgtt agaacaggat
     5521 ggttttctta accgtatcgc gtatccggtg gtgccaccgc atgtggaata tagcctcacg
     5581 ccgttgggcg agcaggtgag cgaaaaggtt gctgcactgg cggactggat tgagttgaat
     5641 ttgcccgagg tgttggcggt gcgggatgaa cgtgcggcat aactgccgga tgcgctgagc
     5701 ttatctggtc tacggtgcat ggctggtagg gcggataaga tgcgccagca tcgcatccgc
     5761 caataaatgc cggatgcggt tgataattta tagaccgaaa aagaagtgtt ttacttactc
     5821 aaatccacct gataaatcgc aaacccgata tcatcggtcg cgactttatt catcggatat
     5881 tgccctttct ctttaataaa cgctgcggct ttatctgacg gggaagtttc gaaacggata
     5941 tccagtttct tatcgccagc tatcggtgct aaacgccagt tgttatctgc cgccgggtga
     6001 atttctcccg cacgtttcga ctcatcagca atccacgctg tcagcaccga gcggttctca
     6061 tccggtgaag caaaagcgat atgtctgtcg cccgtaccgg caaatttgcc gccgtaagcg
     6121 cgatagttat tggtggcaac gaggaacata gcgttcggat caatcggctt gccattaaag
     6181 gtcaggttct taatccgctc tgcattggca ttaatcatct ggcactcgcc gtcatagcgg
     6241 gcgggttggg taacatcaat ctgataattc acaccatcaa taacatcaaa gttataagtg
     6301 cggaaaccat cccagttgat gagtgactgt ggtttcgtgc tgttaggatc aatctggtta
     6361 aactgtccgg cggagcactc cagccactct ttcacctctt taccgctggc tttcactaca
     6421 atcagcgtat tggggtagag ataaagatcg gcggcattac ggaaggtcaa ctggcctttt
     6481 tccacctcca catagcttgc cgggtcattt ttgcgaccac caactttaaa cggtgcggca
     6541 gctgaaagca ccggcagttt tgccagatcc ggatcgccct gaatgtaatg ttcgacatac
     6601 gctttttgcg cgttgttcac gacttgcacg gtcggatcgt cctgcaccag cgccagatag
     6661 ctatacatat tgtcggcaga tttaccgatt ggcttgctga cgaactggcg tgtggcatcg
     6721 tgatcggctt tgagtgtttc taccagcttg ctgtcttccg ccgcgaggga ttttttattg
     6781 gcgatgtcgt aaatcggccg tgcttccgct ttcgcctgcg tcacctgcca tttaccgctg
     6841 tcattactga gttgtaagtc gaccacccca agatgatcgc cccacatgcc cggcattacc
     6901 gccggaacac cattcagcgt gcctttggtg atatcagcct cttcgatatc agcaaaatct
     6961 ttacccggga aaacggcgtg agcatggcca aacataatgg cgttaacgcc cggaatttca
     7021 ctgaggtaat aaactgagtt ttccgccatt actttatacg gatcggcaga cagcccggaa
     7081 tgcgccagaa cgacaacgac atcggcacct ttctcgcgca tttcaggcac gtatttgcgc
     7141 acggtttcgg taatatcatt caccgtcact tttccggata aattagcttt atcccagcct
     7201 atgatttgcg gcggcacgac gccaatatag ccaatcttca gcgtctgttt ttttccgtct
     7261 ttatcgacca cttcggtatc tttaattaaa tacggtgtaa acattggctg tttggttctg
     7321 gcgtcaatga cgttggcatt tacataaggg aatttcgctc ccgccagcgc atttttcagg
     7381 taatccagac cgtagttaaa ttcatgattg ccgagtgtac cgaccgtata atccagcgta
     7441 ttcagcgcct tataaaccgg atgaacatca cctgctttta atcctttcgc cgacatgtaa
     7501 tcggccagcg gactcccctg aatcaaatcg ccgttatcga ccagtacgct gtttttcact
     7561 tcattgcggg catcgttaat caggcttgcc gtacgtacca gtccgaattt ttccgtggcg
     7621 gtgtctttgt aataatcgaa atccatcatg ttgctatgca gatcagtggt ttccatgata
     7681 cgcagatcga ccgtcgctgc attcacactg gcggcaatca gcgtggccag gagcgttgcg
     7741 ctaaacttaa tcatcaggga catcctttta tcatcgggaa tacgaaagaa aagggagaat
     7801 aaacgtctta cttatagaac agtgaagaat gccacaattt tacgctttga aaatgatgac
     7861 actatcacag ttggcgcatt cattaacgat agggtataag taaaacaata agttaacacc
     7921 gctcacagag acgaggtgga gaaatgttag atcaagtatg ccagcttgca cggaatgcag
     7981 gcgatgccat tatgcaggtc tacgacggga cgaaaccgat ggacgtcgtc agcaaagcgg
     8041 acaattctcc ggtaacggca gcggatattg ccgcacacac cgttatcatg gacggtttac
     8101 gtacgctgac accggatatt ccggtccttt ctgaagaaga tcctcccggt tgggaagtcc
     8161 gtcagcactg gcagcgttac tggctggtag acccgctgga tggtactaaa gagtttatta
     8221 aacgcaatgg cgaattcacc gttaacattg cgctcattga ccatggcaaa ccgattttag
     8281 gtgtggtgta tgcgccggta atgaacgtaa tgtacagcgc ggcagaaggc aaggcctgga
     8341 aagaagagtg cggtgtgcgc aagcagattc aggtccgcga tgcgcgcccg ccgctggtgg
     8401 ttatcagccg ttcccatgca gatgcggagc tgaaagagta tctgcaacag cttggcgaac
     8461 atcagaccac gtccatcggc tcttcgctga aattctgcct ggtggcggaa ggacaggcgc
     8521 agctgtaccc gcgcttcgga ccaacgaata tttgggacac cgccgctgga catgctgtgg
     8581 ccgcagctgc cggagcgcac gttcacgact ggcagggtaa accgctggat tacactccgc
     8641 gcgagtcgtt cctgaatcct gggttcagag tgtctattta ctaaattcag atggcagaaa
     8701 cagtgtattt cctgattctg ccatcctgat ttctcccaac ctaaaaagtt ataaataaaa
     8761 agagattgta tttaaagtgc aaaaattcaa ttgctaataa gttacatttt aataatgagc
     8821 gttttttgat agtttacttc tataatgaga tatttaatgg cgacataaaa taaccaaata
     8881 aaataaggta gccatatgtt acccaggatc agacacaata attttattgg tgcggtggag
     8941 ttattcgtaa aatcatcgca tacaaaaaca cattcaaaca atttttttaa taatattcag
     9001 catgcattta agaaaaaaga ttgggtttcg aattatgata gcctgttaac tttgtgggag
     9061 ttttttcgtt gcgcaactca gattgataaa gatagttatc aagtgctttc atcaaaaaat
     9121 gaaactgtta aagccatgga taagtttctt atcagtttca gccttaaaga taacggtgcc
     9181 gaatatacaa tgacattgcg aggcagtgga tttgaatacg aagaaatccc cataacaata
     9241 aatgagtata actcattcat ggatttcaaa aaccgtgaat ttccgttaga acaaaacaga
     9301 agattgtatg cttgggatat tctgcagaaa aaacagtctg acataccgaa aaggattaaa
     9361 ggttatattc accaggcgat tggtgacgtg tctttaggtt atgccttatt ggaggatata
     9421 gtaagcaaat taaaacgggg taaatttgaa ctccaaatac caggaggtgg aattaaagaa
     9481 tgcgatggat ggtatattta cgaaaaaata attgatgata attttgccat agtcatagaa
     9541 tctttgggct ttgcgcttaa aatctatgga ggggatgaac gttttcgtaa tggttcctct
     9601 gttgttttgg aggatgaaga ttactctctt atttataatt ttctggttaa tgcaggttgc
     9661 caacaagtcg aattagccga acaagtggat gcgatagtaa gtgcgaatct ggccgtagat
     9721 agtgatatta ccgaagagga gatttgcgag aaatataaat caaccattga ggcgtttaaa
     9781 aaagagcaac tagcg
//
LOCUS       DAJTMC010000157         9765 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.157, whole genome shotgun sequence.
ACCESSION   DAJTMC010000157 DAJTMC010000000
VERSION     DAJTMC010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9765
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.157"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(188..529)
                     /locus_tag="OO130_003400"
     CDS             complement(188..529)
                     /locus_tag="OO130_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HCR5366676.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVEGLKL
                     IESGDWLDRDK"
     gene            complement(532..4302)
                     /gene="tamB"
                     /locus_tag="OO130_003401"
     CDS             complement(532..4302)
                     /gene="tamB"
                     /locus_tag="OO130_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HCR5366677.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAQEEVVEPKIENPQPDEKPLGETLKDLFSRPVL
                     PEMTDLHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDSNQG
                     IVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGPVDM
                     DLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMRTAV
                     KGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTLNGI
                     NTAKEFPEWPSKLNGLIKTSGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGNSYM
                     QWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKVRGT
                     VEAPQLLADITARGLRWQELSVTQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNINLV
                     TLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTLVGPWSLT
                     RDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDFAMLKPFM
                     PETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTLNLT
                     AELRNNRAELGWTIRLTNNGQFDGQVLVTDPQGRRNLGGNVNIRNFNLAMINPIFTRG
                     EKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMRSTL
                     AGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFEATP
                     NLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNLIVH
                     VGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGELLF
                     SGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSYLLR
                     GQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQVVVS
                     GYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(4299..6032)
                     /gene="tamA"
                     /locus_tag="OO130_003402"
     CDS             complement(4299..6032)
                     /gene="tamA"
                     /locus_tag="OO130_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HCR5366678.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSCGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            6238..6876
                     /gene="msrA"
                     /locus_tag="OO130_003403"
     CDS             6238..6876
                     /gene="msrA"
                     /locus_tag="OO130_003403"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCR5366679.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNEPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            7199..8542
                     /locus_tag="OO130_003404"
     CDS             7199..8542
                     /locus_tag="OO130_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCR5366680.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(8604..8810)
                     /locus_tag="OO130_003405"
     CDS             complement(8604..8810)
                     /locus_tag="OO130_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HCR5366681.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            9135..9689
                     /locus_tag="OO130_003406"
     CDS             9135..9689
                     /locus_tag="OO130_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HCR5366682.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLQKLLK"
BASE COUNT         2256 a         2673 c         2404 g         2432 t
ORIGIN      
        1 tactcaccgt tcaaagcgtg cccccgtttt acccctgcac atttgtgaac gccagcagag
       61 ttttttatgc gattgcagat atggaaagcg gctcggagag aaaagcaaaa gatggaaaag
      121 ttacaaaaaa aaatcgcgag atacatcgca taaaacaacg ccacccgaag gtggcgtgct
      181 tttattatta cttatccctg tctaaccagt cgccgctttc aattagcttt aatccctcga
      241 cgggtcgttg ataaacgtac atccatgcac tcccatacgg cgtctgaatc aactggcgcg
      301 cgtattcacc gcccctggtg cgcaaggcat caagttcggc cagcgtggcg ttgtcaatac
      361 gataaacttc accgtgtacc gttccgttcc ccggaactgc gcctggatag tggcccaggc
      421 tatacaactg gtagttatcg atactgaaat cgcccagtaa ctgggcattg gtcatccagt
      481 gactgttgcc ttgtttgtgg cgtaaactgc cgtagacaaa tattcgcatt gctaaaactc
      541 gaactgatag agcaaatcca gtgcctggtc tacaccagac acggcttcca gatatagctt
      601 aggcatcagg cgataacgta acgtgagtgt tgctatagag tcaaatatac ccacgccgta
      661 tttcacttgc aggcctggca atacatagcc gctgaccact acctgggagg agtcgcctac
      721 tccctgggtg tcgagcgcta aattgcttac gccaaacgtc tcgccgattt tacccacaat
      781 ctggccactt tgcgcaaccc ccagaccaat cagcatcgag gtcattgccg cactgtcgct
      841 ctgatcgctc tccagccctt gtccacgtag caaataagac aatgcagctt gttgcgacat
      901 cgccgggtca gagaagatct ccgctttcgg ttcgtccgcc agaccagtga cgcgaactcc
      961 ggcgattacg tcgtcttctg tagcatccgg gttacgaata gcttcaatat taagatacgg
     1021 ttgatctggc ggaccagaga acagtagctc acccttacgc acaatcagat cctgaccata
     1081 ggcatggaag cgcccttcag ggatgttgat ctgcccgttc aggcccagcc cttgtttgtc
     1141 ctgaacgacg ttaagatcgc ccgtcagccg cgctttcagg ccaaaggcgt caatgcgcac
     1201 attgttgcca acgtggacaa tcaggttact gttaatcgga atcgacgccg ttttcggctc
     1261 ttccggttgc aggttatcgt taagcatcac cacatcgctg gagacgccta ctgcgctttc
     1321 cggcagatcg tgcaccacga tgcgcgccca cgggacatcc acgcgaccat cgagggtaaa
     1381 caggtttggt gtagcctcga atacaacatc tggcgataca tccattcgta ccatcggcgg
     1441 cacggtgatc cgcactttac tgcctttcgc cgttactcgt gcccgccagt tttcaatttg
     1501 gctccagtcg gcatcaccgt tcaggtagat ttcaccctgc tgggtccgta ctgtaccggc
     1561 aagcgtcgag cgcataccgt taaagttgac cgcaagctgg ctcggctgca tatcaaacgg
     1621 cataaagttg ccgtcgatat ccacacccgt aacctgaagc tgaccaaaca actgcgggct
     1681 ttgcacatca ccacccagac gcaagttggc actcaccatc cccgctgctt tttccccacg
     1741 agtaaagatg gggtttatca tcgcaaggtt gaagttacgg atattgacgt tgccaccaag
     1801 attacggcgg ccttgcggat cggtcaccag cacctgtcca tcaaactggc cgttattggt
     1861 caggcggatg gtccagccca attcggcacg gttgttacgc aattccgccg tcagattcag
     1921 tgtctgaaac gccaccggca gcgccgcatc gttgacggtt tgcgttacct gcacgttacg
     1981 ccccgaaagg gtgatactgc cctgcggcag cccctctttc gtggtgtccc aggcaacatc
     2041 cgctttaccc gtgaagatac cgctggcctg agtggtttct ggcataaacg gtttcagcat
     2101 ggcgaagtcg aagcggttga gattcaccac cgcacgccct tcggcccccg catcgatagt
     2161 ttgcggcacg cacagttccg cattcgggtt aagccagcag tgtggcccga tgctgatttt
     2221 ttgctcctta ttgcggtaat ccagcgcaat atcgcgggtc agcgaccacg ggccgaccag
     2281 cgtctggaag cgggtattgc taagagttcc cttccagcgt tcttctttgc gatcaaaact
     2341 tcctgccaga ttaagctgcc cggagacagg ctcgccctga atccgcaact gtagctcgtg
     2401 ctgcttttcg ctgcctttgg cattcagggt gacgaggttg atatttacat ccggctgcga
     2461 aatttgctca acgcgtacgt cgagtttccc ggcgatctga tcggtggatt taatgtcgcc
     2521 ttccacgcga acctgcgtca cggaaagttc ctgccagcgc aggccgcgcg cggtgatatc
     2581 tgccagtagt tgtggcgctt ccaccgtgcc gcgtactttc accagccctt tcgctgtacc
     2641 gccaagcccc ggcagcgcgt tatccagccc cggcgcgttg atggtggcat caagattgag
     2701 atcttttacc cccagctcgc ctttcacttc ggcactgttt ggccccagtt ccagatgaag
     2761 cccagggatc atccactgca tataactgtt gcctttcagc gtgccgtcaa cgttcacttt
     2821 gttctgtttg acgttaccgg tcagcttcag ttctggcacc tccatctgcc aggtgccgcc
     2881 gtacaggcta ccgctggttt taatcaagcc attgagtttc gacggccact ccgggaactc
     2941 tttggcggtg ttaatgccat taagcgttag ctcaccgcgc caactaatgg cctgctgcca
     3001 gtcgagcaac gccttgagtt cagttttccc ttccagcgcc gcgacggtga gtttgtcgag
     3061 attgacctgc tgttcattac ctttggcatc aagggtaatg gtggctggcg ggatctcctg
     3121 tcccttcact gccgtacgca tagagagcgt gtaatcggtc attttgccgg taagtttcag
     3181 tttcagatca tccgcctgat actgcttctc accagtgaac ggccagtaaa gctgtttgct
     3241 gttcacttcc acgttgagcg gcaatccggc ttccgccagt cgcgtctggg cgcgtaaatc
     3301 catatccacc ggaccggaaa gattaacgcc aatctccagc tgttcgcgca gcgcgccgcc
     3361 cacttttagc ttcacttttt cacctttcaa cggctccacg ttcagtgtgc tgttgagggt
     3421 gatatccacc ggccagttgt ctgacagctg cgccgtgccg ctggcgttga cgatcccctg
     3481 gttggaatcg atatccaggg cgtccagttt agtattgccg tcaatgctgc tcactttcag
     3541 cagcatggtg cgcacggtga tgtccgtgtc gcccgtcacg cgtaactgct cgcctttaaa
     3601 ctcttcaatg ttcaggttaa gcggcaaatg caagtcggtc atttccggca ataccgggcg
     3661 agaaaagaga tctttcagcg tttcgccgag cggcttttca tccggctgcg gattttcaat
     3721 tttcggttcg accacttctt cctgcgccac tttcggcaga gcaatcagca ggcctttcag
     3781 cgacgtcggt ttcagggtca gggttttctc ctgccagttc aggccggagg tgaagtccat
     3841 caccgacacc gtggtgtcat caatcttgat gttgacgttg tccagcgcca cccgtgtcag
     3901 ggtgatggga tacggcgtgg agagatccag cggaccgcta tcttcctctt cttcaacctg
     3961 ttcagaagga ggcatttttt tactgtcgat gttgacctga atgtctttca gcgccaggtc
     4021 attaatacaa acgctactgt tccacaggca ctcaagaccg accgccagat gcagattacc
     4081 cgcttttacc gccacgcctg gctgctcata acgaacgtca gacaaggtga gatcgcgcca
     4141 gccgccggtg accttgccaa tatccagtcc tggcacccag cgatccgccg ctttaaatac
     4201 aagatgcagg ccgctggtgg tgcccaccag aaacgccacc gatcccagca acagtaagat
     4261 aacgataacc acgccgaggc tgattttttt ccataaactc ataattctgg ccccagaccg
     4321 atgtaaaact gtaacccgtg ttcgtcttta tccgcgaccg gtacggcaaa atcgagtttg
     4381 attggcccga ccggcgattc ccagcgcacg ccgaccccgg taccggtttt aaagtcgctg
     4441 cggcgaatat cgcttaccgc ttcgccacta tcgacaaaca ccgcgcccca ccattttccg
     4501 gtcacgttgt actggtattc cagcgatccg gttatcaact tcgaggcccc tttcaggtca
     4561 ccgttggcgt atttcggggc gatagatttg tatttgtagc cgcgaatact gcgatcgccc
     4621 ccggcgaaaa aacgcagatc cggcggtact ttgtcgaaat caccggtttc aatccagccc
     4681 agcgtgccgc gtgtaacgaa acgatggcga tcgtacagtg tgcggatcca gacgttctgc
     4741 gcctggaaaa cggagaaatc gacatctgag ccccaggccg tgttggagta gtcgatagag
     4801 tagcgttgcg agtcgcccca ggttggcatc aggccaccac aagaacgcgt gcggctaatc
     4861 atcaccccag gataaaacag catcgtggtg ttggtaattt caccctgagt aaagtggtcg
     4921 agactccagc gcaggttaat ggcacgctgc caaccgctgg agagatccca gtagcgagaa
     4981 gccaccagcg tagtggagtc agattcggta tcgttcaggt cagtgcgctt aaaaccgccc
     5041 tgcaccaaat aatattgttc cagtggattc ttcagcagcg gcattttata gctgaagtcg
     5101 agggtctgtt ccggcgcgga aatactggta ctggtggtca aactgtgacc gtatgagttc
     5161 atccacggct ttttccacgt cgctttcacg cgcggtccca cgtccgtaga gtaaccgacc
     5221 ccggtttcga tggtgttttc tgttcgcggc gaaaccacgc ccgtcaatgg taatactttc
     5281 gtttcgcgcg ctttatcaaa ttgtggagcc accaccaccg agttaaacca gccggtagca
     5341 gaaagtcggc ggttcagttc cgccagatct ttcgattcat actcatcgcc ctctttaaac
     5401 ggcaccagat tttgcaggta ttcatcgcgg atttgtgatc cttcaaaggt cacatgccca
     5461 aagcggtaac gttcgccact gttataatca atatcccaga aggctttatg caggccgagc
     5521 gcaatgccca gctgcgcttt ggtaaattcg ctatcgaaat aacctttacg caacgcaata
     5581 ctggttaagg actttttgaa attttcataa tcgccctggt tcagtaccgt accaatagcc
     5641 gggcgagtat cgagcaattt caaatagtct ttatcggtcc gcgcgccgcc gcgcaatacc
     5701 acatcggtgc cgccaattaa caccggcaca cctggcgtga ctttggcgat caatacctgc
     5761 cgccctttct ttggcggtgg acggagatca aattcaatgg tcggctggta ataacccagc
     5821 gctttcagac cttcacggat ggcatcatcg acgcgtgcgc gaaagcggcg gtctggcgtc
     5881 acttcatcac tttcaatcgt agaaagctgc gcacgaacgt tcttttccag ctgtcccgat
     5941 aacccctcga cctgtagacg gacgttcgcg gcgacggcag atccgcttaa gcagagtaag
     6001 cttacacagc ataactgtcg gatatagcgc acattttctc ctgaatatcc ttttttcctg
     6061 cccctggaac gccgttaaac ggcttaacaa aaatccagta atatggatta aaaagcagac
     6121 taaaccccaa atatttctta tgttttactt tagacctatt cacggtggtt attgtgtgca
     6181 aatacgcctc ttgttacaac cttaacccca atgaccgatt ttcgggagag cgacaccatg
     6241 agtttatttg ataaaaagca tctggtttcc cccgccgatg ccctgcctgg acgtaacacc
     6301 ccgatgcccg tagccacgct gcatgcggtc aacggtcact caatgaccaa tgaacctgac
     6361 ggaatggaga ttgccatttt tgcgatgggt tgtttctggg gtgtggagcg tctgttctgg
     6421 cagttacccg gcgtttacag caccgccgca ggctataccg gcggctatac gccaaatccg
     6481 acttatcggg aagtgtgctc cggtgatacg ggtcatgccg aagcggtacg cattgtttac
     6541 gatccttccg tcatcagcta tgagcagttg ctacaggtat tttgggagaa tcacgatccc
     6601 gcccagggaa tgcgtcaggg caatgaccac ggcacgcagt atcgttcagc gatttatccg
     6661 ctgaccccag aacaggatgc cgcagctcgc gccagtctgg aacgttttca ggcggcgatg
     6721 cttgccgccg atgacgatcg tcacatcacc acggaaatcg ctaacgccac accgttttat
     6781 tatgccgaag atgaccacca gcaatatctg cataaaaacc catatggtta ctgtggaatt
     6841 ggcggaattg gcgtctgtct gccgccggaa gcatagcgtt acgggtacaa atgtagtttg
     6901 ttgataaagt gctctttgtc tatgcctgat gcggcttaaa cgacttacca ggcctacaaa
     6961 atcgtccaaa ttcaatatat tgcaaggact gcgtaggcct gatcggcata gcgcatcagg
     7021 cagtattgca tttatcagca gtctgaattt taaccctctg gcgactttac agtaccttac
     7081 gctatactag ccactgaaaa ttccggttca ctttcttcga atcggctttc aatgtgtatt
     7141 tcacacaaat taatcaactt tcccttccga ggatctggcc tgaaaggtcg gataagatat
     7201 gttaaacagt attttagtca tactctgctt gatcgctgta agtgcgttct tctcgatgtc
     7261 cgagatctcg cttgccgcct cacgcaaaat caaacttaaa ctgctggctg atgaaggcaa
     7321 tataaatgcc caacgcgttc tgaatatgca ggaaaatccc ggcatgttct ttaccgtggt
     7381 ccaaatcggt ctgaacgcag tggcgattct cggcggtatc gtcggtgatg cggcattttc
     7441 tccagctttt cacagcctgt tctcccgcta tatgtcggca gagctctctg agcaactgag
     7501 ctttattctc tctttctcgt tagtgactgg catgtttatc ctgtttgcgg atttaacccc
     7561 gaaacgcatc ggtatgattg cgccagaagc ggtggctttg cgtatcatca acccgatgcg
     7621 cttctgcctg tacgtttgca ccccgctggt gtggttcttc aacggcctgg cgaacatgat
     7681 cttccgtatt ttcaaactgc caatggtacg taaagatgac atcacttctg atgacatcta
     7741 cgcggtagtg gaagccggtg cgctggcggg cgtgttacgt aaacaggaac acgagctgat
     7801 tgaaaacgtc tttgagctgg aatcccgtac cgttccgtct tcaatgacac cgcgtgaaaa
     7861 cgtgatttgg tttgatctcc acgaagatga gcaaagcctg aagaataagg tggcggaaca
     7921 tccgcactct aagttcctcg tctgtaatga agatattgac cacatcatcg gttatgtcga
     7981 ttctaaagac ctgctgaacc gcgtgctggc taaccaaagc ctggcactga acagcggcgt
     8041 acaaattcgc aacacgctga ttgtgccgga tacgttaacc ctttcagagg cgttggaaag
     8101 ttttaaaacc gcaggtgaag acttcgcggt gatcatgaac gagtacgcgc tggtggtggg
     8161 gatcatcacc ctcaacgacg tgatgaccac gctgatgggc gatctggtcg gtcaggggct
     8221 ggaagagcag attgtcgccc gtgatgagaa ctcatggctg attgacggcg gcaccccaat
     8281 tgacgacgtc atgcgcgtgc tggatattga cgagttcccg cagtcgggca actacgaaac
     8341 catcggcggc tttatgatgt ttatgctgcg taagatcccg aaacgcaccg attcggtgaa
     8401 attcgccggc tacaaatttg aagtggtgga tatcgataac taccgcatcg accagctgct
     8461 ggtgacccgg atcgacagca aggccaccgc cctttcgcca aaactgcctg acgctaaaga
     8521 taaagaagaa agcgtcgcgt aacccccaga aacatcaacg gctcctgaat caggagccgt
     8581 cttattactg catagcactt tggttaagcc atctctgttt gcagacgcat aacctgacgg
     8641 ttaacttcgg acatcactga caaatgcagc ttatccttca cttttgggat aagaatctta
     8701 cccttatcaa attcaaaagc gccaacgtcc ttgatgtata accgtccacg gaacaggatc
     8761 tttacgtact tcgccacctg aagtgggttg taccgttgga aaattttcat tgttatctcc
     8821 tgctgagtat tacgcctgtg cggtgccaca atcggcccaa ctattatgag acgcaaattt
     8881 taatgcttag tgactataga ctatccgggc aatgtttcca ccgcgtataa ctttttttac
     8941 ctaaaggtta caattattca gaattatctt tttaccgaag cgcggtcttc agtataagca
     9001 ttcatttttc atatgatttg tgcgcttgac cgcaaactgg catcacactt gcgggaaatt
     9061 cgataaatag cacatatgat taaaactcag atccaagtgg tcggatcacc tgcatatcat
     9121 aagaaggaaa caccatgacc ctacgcaaga ttctggcact cacctgcctg ctgttgccga
     9181 tgatggcttc cgcacatcag ttcgaaaccg gtcagcgagt gccgccgatt ggcattaccg
     9241 atcggggcga gttggtgctt gataaagatc agtttagcta caaaacctgg aacagcgcgc
     9301 agttagtggg aaaagtgcga gtactgcaac atattgctgg tcgcacctct gcaaaagaga
     9361 aaaacgcgac gctgattgaa gcgattaaat cagcaaagtt accgcacgat cgttaccaga
     9421 ccaccaccat tgttaacacc gacgacgcaa ttccgggttc cggcatgttt gtgcgcagca
     9481 gtctggagag taataaaaag ctttatccct ggtcgcagtt tattgtcgat agcaatggcg
     9541 tcgcgcgcgg tgcctggcag ctggatgaag aaagttccgc tgtagtggtg ctggataagg
     9601 atggtcgcgt gcaatgggcc aaagacgggg cgctcacaca ggaagaggtg cagcaagtga
     9661 tggacctgct gcaaaagttg ctcaaataat ggcaataaca taaattctgc aaaaataatt
     9721 ttcccctatc gttatctatg ggatagggga attaggtctc tgagc
//
LOCUS       DAJTMC010000158         9740 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.158, whole genome shotgun sequence.
ACCESSION   DAJTMC010000158 DAJTMC010000000
VERSION     DAJTMC010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9740
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.158"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            <1..386
                     /locus_tag="OO130_003407"
     CDS             <1..386
                     /locus_tag="OO130_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="immunoglobulin domain-containing protein"
                     /protein_id="HCR5366683.1"
                     /translation="LAEVRQHTWSSGTNGVVAATFSLRLKGKPVSFVVPLAFVKNLDK
                     TLTVNTGALLTMSVSANGGMPPYKYAWKKDGQPVDGQTTDTFSKPGAQSADAGKYTCV
                     VTDSAEKAQSVTSVECTVTVSAAAG"
     gene            401..775
                     /locus_tag="OO130_003408"
     CDS             401..775
                     /locus_tag="OO130_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HCR5366684.1"
                     /translation="MKKDLKTLALARLSGFRHKTVKVPEWGNVSVVLREPSAEAWYLW
                     QEVLNGDGEDDDTLSVVAKTRRNLEADVTLFCDVLCDTDLQRVFTPDDREQVLAVYGP
                     VHARLLRQALELIADAESARKK"
     gene            871..1080
                     /locus_tag="OO130_003409"
     CDS             871..1080
                     /locus_tag="OO130_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4035 domain-containing protein"
                     /protein_id="HCR5366685.1"
                     /translation="MWMEFDRISPLGDERGDIRNAQIVKAVFGAQGMNVALKDVMLCW
                     GEDEDKPEVDPFAALEDALSFAAQS"
     gene            1128..1388
                     /locus_tag="OO130_003410"
     CDS             1128..1388
                     /locus_tag="OO130_003410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5366686.1"
                     /translation="MATLRELIIKIVSFRGTHIDAFFRRASSAAHTNARGLITVDLFC
                     CTEAIARQVPEAGSEVLSSVIASGREVLTRGLIAGDDVLTRE"
     gene            complement(1523..3124)
                     /locus_tag="OO130_003411"
     CDS             complement(1523..3124)
                     /locus_tag="OO130_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISEc49 family transposase"
                     /protein_id="HCR5366687.1"
                     /translation="MNIRIWSGILPCMDISALNTTNDIEKLRAMALAMVQEVMSENAE
                     KERELLEKSRRIQLLEEMLKLVRQQRFGKKCETLAGMQRSLFEEDVDADIAALTAHLD
                     KLLPQSPEEDEKASRSRPIRKPLPVHLPRVEKMIQPDTDHCPECDEPLHYIRDAVSEK
                     LEYIPAHFVVNRYVRPQYSCPCCQKVFSGEMPAHILPKSAVEPSVIAQVIINKYGDHL
                     PLYRQQQVFARSDVGLPVSSMADMVGAAGAALSPLAALLHRELINRPVVHADETTLKI
                     LNTKKGGKSCSGYLWAYVSGERTGPSVVCFDCRTGRSHEYPENWLQGWGGTLVVDGHK
                     AYRTLANKVPEITLAGCWAHARRGFADLYKISKDPRAAIAVKKIAGLYRLEKKISSRP
                     VEKIRQWRQRYARPILEELWSWLEEQEPQCSPGKALHKAIAYALSHRVELSRFLEDGA
                     VPLDNNVCERAIKNVVLGRKSWLFAGSQMAGERAAQIMSLLETAKRNGLEPHAWLTDV
                     LMRLPEWPEERLAELLPLEGFTFSG"
     gene            complement(3131..3523)
                     /gene="tnpB"
                     /locus_tag="OO130_003412"
     CDS             complement(3131..3523)
                     /gene="tnpB"
                     /locus_tag="OO130_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR5366688.1"
                     /translation="MLSPDNVFIAIKPVDMRRGIDSLTQYIQDELRSTWHEGAAFVFV
                     NKVRSRIKVLRWDKHGVWLCTRRLHKGSFRWPRANDAAWHLTPDEFNWLIAGVDWQQV
                     KGHDLTKWVWQNEPELRPENTQNTLLTQ"
     gene            complement(3510..3839)
                     /locus_tag="OO130_003413"
     CDS             complement(3510..3839)
                     /locus_tag="OO130_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HCR5366689.1"
                     /translation="MSKPRWTIEQKRQHVAAWRASGLTRQQYCELNDIPFTSLREWPQ
                     DVAKAERRANEPTVLPVHIAPPLHADVPQPVTNEPVTLFLPGGVRMCCQPSQLTDVFR
                     ALKYAQS"
     gene            3884..7144
                     /locus_tag="OO130_003414"
     CDS             3884..7144
                     /locus_tag="OO130_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR5366690.1"
                     /translation="MTEDPCEDDFPVTTLKISANSQSFQSEIQRASRMGSEYYRTLQN
                     GGRHAAAAAREQRRALAELNSQLTEIRGSAVGMAGAFAGAFATEHLISLADEWGSVNA
                     RLKQASQSSDEFSSSQKVLMDISQRTGTAFSDNAALFARSAASMREYGYSADDVLKVT
                     EAISTGLKLSGAGVAESGSVITQFSQALAQGVLRGEEFNAVNENGDRVIRALAAGMGV
                     ARKDLKAMADDGKLTADKVVPALISQLGILRDEYAAMPETVSGSITKVENAFMAWVGE
                     VNEACGVTKTLSGVLNGVAGQIDNVATAVGALVAVGVARYFGNMASGAMSATAGLVTA
                     ARNEVALAEAQFRGTQIATARARAAVYRAQQAVAAARGTEMQIAAEARLAATQERLNR
                     NIAARSAAQNALNSTTAVGSRLMSGALGLVGGVPGLVMLGAAAWYTLYQNQEQAWESA
                     RQYALTIDEIAHKTPSMSLPEASDNEGRTRAALTEQNRLIDEQASRVKSLQEKIAGYQ
                     YVLANPGWTTGDGFMINHLTSVKTVTEGLAQATEQLAVEQSRLALMQEKAQSIQDVLA
                     GLEDRRVALIRQQAAEQNKVYQSMLVMNGQHTEFNRLLGLGNELLQQRQGLVNVPLRL
                     PQATLDDKQQSALTKTERELALSRLKGEEKERVRLGYAADDLGFVGDPYQEARQRYIS
                     NALEAWRNNEANKPKSRGGKSETEKAEDSFSRLLKQQKEQLALAGQNTELAKLKYQTA
                     QGELKTLTEMQKQELLRNAALIDQQKIREQLRSREETLKNDNVAARASNEAELLGYGQ
                     GERLRERMRELQQIRDSFRQKDADLQSQYQTGDISEDFYRQALAQNAQYLSERLKEQE
                     AFYAESDVQRADWQKGLQEGFSNWVDNASDYASQAAQLATEGISGMVNNITEMLNGNK
                     VEWRSWASSVLQEISKVLMNAAIVNGIKTAANSMSGAGGFIGSIGSWLGGAVANAKGG
                     VYTSANLSAYSNSIVDTPTYFAFAKGAGLMGEAGPEAIMPLTRAADGSLGVRAVGSMN
                     GSAGLVYSPVYHIAIQNDGTNGQIGPEAAGSLVQLIDQRVQAVMLSMRRDGGMLSG"
     gene            7137..7478
                     /locus_tag="OO130_003415"
     CDS             7137..7478
                     /locus_tag="OO130_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5366691.1"
                     /translation="MAEIKTLHLVPREGMQVSEKPSVARVRFGDGYEQRRPTGLNARL
                     KTFQAVFRVTDEATRRWLEEFLSWHGGYRAFLWRPPKHNRTVRVVCREWSVTDNARYS
                     DFSCTIEQVVN"
     gene            7478..8176
                     /locus_tag="OO130_003416"
     CDS             7478..8176
                     /locus_tag="OO130_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HCR5366692.1"
                     /translation="MQDIHEESLSESVKSEQSPRVVLWEIDLTVQGGERYFFCNELNE
                     KGEPVTWQGRKYEAYPIDGSGFEMNGRGSSARPSLTVSNLFGLVTGMAEDLQSLVGAT
                     VVRRRVYARFLDAVNFVAGNPEADPEQELSDRWVVEQMSQLTAMTASFVLATPTETDG
                     ALFPGRIMLANTCMWTYRSDECGYTGGAVADEFDKPTTDIRKDRCSKCMRGCELRRNV
                     GNFGGFLSINKLSQ"
     gene            8187..8930
                     /locus_tag="OO130_003417"
     CDS             8187..8930
                     /locus_tag="OO130_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5366693.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVISTPEGERYIPCVNISAEPE
                     AYFRIAPEDWLRAEMQGEIVALVHSHPGELPWLSEADRRLQIKSALPWWLVCRGEIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIDMPDFHREDDWWRNGQNLYLDNMAVT
                     GFYRVPLSSAPPGDILLCCFGASVPNHATIYCGNGELLHHLPEQLSKRERYSEKWQRR
                     THSVWRHRHWHASAFTGICNDLAAASACM"
     gene            8828..9508
                     /locus_tag="OO130_003418"
     CDS             8828..9508
                     /locus_tag="OO130_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000246273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HCR5366694.1"
                     /translation="MATTNAFCLASPPLARICLHGDLQRFGRRLSLYVNTAAEAIRAL
                     SMQMPGFRRQMNEGWYQIRIAGDDTAPEAVYARLHEQLGEGTVIHIVPRLAGAGKGGL
                     QIVLGAAAIVGSFFTAGASMALWGSALAAGGFSATTMLFSLGASMILGGVAQMLAPKA
                     KTPDYRATDNGRQNTYFSSLDNMIAQGNPMPVPYGEMLVGSRRISQDISTRDEGGGGK
                     VVVIGRQG"
BASE COUNT         2176 a         2389 c         3074 g         2101 t
ORIGIN      
        1 tcctggcgga agtgcgtcag cacacctggt catccggtac caacggtgtg gtggctgcaa
       61 cgttctcact gcgtctgaaa ggcaaaccgg tgtcctttgt ggtaccgctg gcgtttgtga
      121 aaaatctgga taagacactt accgtgaata ccggtgcgct gctgacaatg tcagtcagtg
      181 ccaacggggg aatgccgccg tataaatacg cctggaagaa ggatggtcag ccggttgacg
      241 ggcagacgac agacaccttc agtaagccag gtgcgcagtc cgctgatgcg gggaaatata
      301 cctgcgtggt gaccgattcg gcagagaaag cacagagtgt gacgtctgtt gaatgcaccg
      361 tgacagtgag cgcagctgcc ggataagggg atgggtcatc atgaaaaagg atctgaaaac
      421 gctggcgctg gccagactgt cagggtttcg tcataaaacg gtgaaggtgc cggaatgggg
      481 gaatgtcagc gtggtgctgc gggagccttc ggcagaggcc tggtatctgt ggcaggaagt
      541 gctcaatggt gatggagagg atgacgatac cctgtcggtg gtggcgaaaa cccgccgtaa
      601 cttggaagcg gatgtgacgc tgttctgcga tgtcctgtgt gatacggacc tgcagcgggt
      661 gttcactccg gacgaccgtg agcaggtgct ggccgtctat ggtccggtac atgcccggtt
      721 gctgcgtcag gcactggaac tgatcgctga tgcagagtcg gccagaaaaa agtagcccgc
      781 ccggaaattc gctttctgat gcgacttgcg ctccgtctgg ggcgcacctt atccgaactg
      841 cgccacagcc tgagtgcgag cgaggcgatg atgtggatgg agttcgacag gatatccccg
      901 ctgggtgatg agcgcgggga tatccgtaat gcacagatcg tgaaagcggt tttcggggca
      961 caggggatga atgttgcact gaaggacgtc atgctctgct ggggcgagga tgaggataag
     1021 ccggaggtgg atccgtttgc ggcgctggaa gacgcgctga gctttgcagc acagtcatga
     1081 atgatgagaa ccgctgaggc ggttttttta cgcccggaga aaggtgaatg gcgacgttac
     1141 gtgaactgat tatcaaaatt gtaagcttcc ggggaactca tattgacgcg tttttcaggc
     1201 gcgcgtcatc ggcggcgcat acgaacgctc gtggtttgat aacggttgat ttattttgtt
     1261 gcacagaggc gatcgccagg caggtaccgg aggcgggctc tgaagtactg agtagcgtga
     1321 tcgcgtcagg ccgcgaagta ctgactcggg gattaattgc aggtgatgat gtactgaccc
     1381 gggaatgact ccgggctgat tcaggttctt attcgctgtc agcccgcgtg gttgctggtc
     1441 tggctcataa cctgcagtca ggagttcaac tcccgactgc aggttatggc ccggtgcacg
     1501 aacacctgac ggcaggtatc actcacccgg agaaggtaaa tccctcaaga ggcagcaact
     1561 ctgccagtcg ctcctccggc cactccggca gacgcatcag gacgtctgtc aaccaggcat
     1621 gcggctccag accgttgcgt ttcgcggttt ccagcaagct cattatttgc gcggcgcgtt
     1681 ctcccgccat ctgcgaaccg gcgaacagcc acgattttct gcccagaacc acgtttttga
     1741 tggcccgttc acacacatta ttatccagcg gcaccgcacc atcttccagg aagcggctca
     1801 gttccacgcg atgagacagc gcataggcaa tagctttgtg taatgccttt cccggagaac
     1861 attgcggttc ctgctcttca agccatgacc acagttcttc cagtatcgga cgggcataac
     1921 gctgtcgcca ctggcggatt ttttccacgg ggcggctact gatcttcttc tcaagacggt
     1981 acaaccccgc gattttcttc acggctatgg cagcccgtgg atctttactg attttataca
     2041 ggtcggcgaa gcccctgcgg gcatgggccc agcatccggc cagcgtgatc tccggcactt
     2101 tgtttgccag agtccggtaa gctttatgtc cgtcgacaac cagcgtcccg ccccagccct
     2161 gaagccagtt ttcaggatac tcatggctac gtccggtccg gcagtcgaag cacacaactg
     2221 acggtcccgt cctttctcca ctgacgtatg cccacagata accggagcag gatttaccgc
     2281 ctttcttcgt gttcaggatc ttcagggtag tctcatctgc atgcaccacc ggacggttta
     2341 tcaactcgcg atggagtaac gccgccaggg gagataatgc ggcacccgcc gcgccaacca
     2401 tgtcagccat cgaactgacg ggcagcccga catctgaacg ggcaaagacc tgttgctggc
     2461 gatacagagg caggtggtca ccgtatttat tgatgatcac ctgtgcgatg actgatggct
     2521 caacggcact tttcgggagg atatgtgccg gcatttcacc gctgaacacc ttctggcaac
     2581 agggacaact gtattgcgga cggacataac ggttcaccac aaagtgagcg ggaatatact
     2641 ccagcttttc actcaccgca tcgcggatat agtgcagcgg ctcgtcacat tcagggcaat
     2701 ggtcagtgtc cggctggatc attttttcca cccgtggaag atgaaccggt aagggtttgc
     2761 gtatcgggcg tgaacgggac gctttttcat cttcttcagg ggattgcggg agcagtttat
     2821 ccagatgtgc ggtaagcgcg gcgatatcgg catcaacatc ctcttcgaat agggagcgtt
     2881 gcataccagc cagcgtttca catttttttc cgaagcgctg ttgacgaacc agtttcagca
     2941 tttcttccag aagctggatg cgccggcttt tctccagtaa ttcccgctct ttttcggcat
     3001 tctccgacat gacttcttgt accatggcaa gtgccatagc acgcagtttt tcgatgtcat
     3061 tcgtggtgtt gagagcggag atatccatac aggggagtat accactccag atacggatat
     3121 tcattatttt tcactgagtt agcagtgtat tttgcgtgtt ttcagggcgc agttcaggtt
     3181 cattctgcca gacccatttc gtcaggtcat gtcccttaac ctgctgccag tcaacaccgg
     3241 caatcagcca gttaaactcg tcgggagtga ggtgccaggc agcgtcattt gcacgtggcc
     3301 agcggaagct gcctttgtgc agacggcggg tacacaacca caccccgtgt ttatcccacc
     3361 ggagaacttt gatacgcgaa cggactttgt taacaaagac gaaggcggct ccctcatgcc
     3421 atgtcgaccg gagttcatcc tgtatatact gcgtcagtga gtcgatgccc cggcgcatgt
     3481 caacaggttt aattgcgatg aagacattat caggactgag catatttgag tgccctgaag
     3541 acatcggtaa gttgtgacgg ctgacagcac atccgtactc caccgggaag gaagagcgtg
     3601 actggctcat tcgtgacggg ctgtggtaca tcagcatgca gtggtggcgc aatatgaacg
     3661 ggaagaacgg tcggttcatt tgcccggcgt tcagcttttg cgacatcctg cggccattcg
     3721 cgaagtgatg tgaagggaat atcgttgagt tcacagtatt gctgacgtgt aagaccactg
     3781 gcgcgccagg cagcaacgtg ctgccttttc tgttcaatgg tccatcgggg ttttgacatc
     3841 gattatctcc ggtaagttgg tgaatggatg tcggtaatac taactgaccg aagatccctg
     3901 tgaagatgac ttccccgtaa cgacactcaa aatttcggca aattcacagt cattccagtc
     3961 ggagatccag cgggcgtccc gtatgggcag tgaatattac cggaccctgc agaatggcgg
     4021 acgtcatgcc gctgctgccg cacgggaaca gcgccgcgcc cttgcagaac tgaacagtca
     4081 gttgacggaa attcgcggtt ctgctgtcgg aatggctggc gcatttgccg gtgcctttgc
     4141 caccgaacac ctgatttcgc tggccgatga gtggggttcc gtgaatgccc gtctgaaaca
     4201 ggcatctcag tcatcggatg aattctcgtc atcacagaaa gtgctgatgg atatcagtca
     4261 gcgaacaggt accgcgtttt cggataatgc ggccctgttt gcccgttcgg ctgcctcgat
     4321 gcgtgaatat ggttacagtg ctgatgatgt gctgaaggtg acggaggcca tttcgacagg
     4381 actgaaactg tcaggggctg gagttgcgga atccggttcg gtgatcaccc agttcagcca
     4441 ggcactggca cagggtgtac tgcgtggcga agaatttaat gctgttaacg aaaatggcga
     4501 ccgggtgatc cgcgcgcttg ctgcggggat gggtgtggcc cgtaaagatc tgaaggcaat
     4561 ggcggatgac ggaaaactga cagcggataa agtggtccct gcgttaatca gccagctggg
     4621 gatattacgt gatgaatatg cggccatgcc ggaaacggtt tccggtagta tcacgaaggt
     4681 ggaaaacgcc tttatggcct gggtgggcga agtgaatgag gcctgcgggg tgacaaaaac
     4741 gctctccggc gtgctgaacg gtgttgccgg acagattgat aatgtggcaa cagctgtggg
     4801 ggcgctggtt gccgtcgggg ttgcccggta ctttggcaat atggcctccg gagcgatgtc
     4861 tgccacggca ggacttgtga cggctgcacg taatgaagtt gcactggcgg aagcacagtt
     4921 caggggaacg cagattgcca cggcgcgggc aagggcagcc gtgtaccgtg ctcagcaggc
     4981 cgtggcggca gcccgcggga cggagatgca gattgctgca gaggcccgtc tggcggccac
     5041 acaggaacgc ctgaacagaa atattgctgc cagaagcgcc gcccagaatg cgctgaacag
     5101 tacaacggcg gtgggctcac gtctgatgag cggtgcgctg gggctggttg gtggcgtacc
     5161 cggactggtg atgctggggg ctgcagcatg gtacacgctg taccagaatc aggagcaggc
     5221 ctgggagtct gcgcgccagt atgcactgac gatagatgaa atcgcgcata aaacgccgtc
     5281 aatgtctttg cctgaagcct cagataatga aggacgaaca cgggcggcgc tgacagagca
     5341 gaaccggctg attgatgaac aggctagtcg ggtgaaatcc ctgcaggaaa aaatcgcagg
     5401 atatcagtat gttctggcga acccgggctg gacgaccggt gacggattca tgataaacca
     5461 tctgacctcg gtgaagaccg taacggaagg gcttgctcag gcaacagagc agcttgccgt
     5521 tgagcagtcc cgtctggcac tgatgcagga aaaagcgcag tccattcagg atgtgcttgc
     5581 cgggctggaa gaccgtcgtg tggcgttaat tcgtcagcag gcggcagagc agaataaggt
     5641 gtaccagtcc atgctggtta tgaacggtca gcatacggaa ttcaaccgtc tgctggggct
     5701 gggtaatgaa ctgcttcagc agcggcaggg actggtgaat gtgccgttac ggctgccaca
     5761 ggccactctg gatgataaac agcagagtgc cctgacaaaa acagagcgtg agctggccct
     5821 gtccagactg aaaggggaag aaaaagagcg tgtccgactg gggtatgcgg cggatgacct
     5881 cggttttgtg ggtgatccgt atcaggaggc gagacaacgt tatatcagta atgccctgga
     5941 agcctggcgc aataacgagg cgaataaacc caaatcccgg ggtggaaaat cagagacgga
     6001 aaaagcggaa gacagttttt cccggctgct gaagcagcag aaagagcaac tggcactggc
     6061 ggggcagaat acagagctgg cgaagctgaa ataccagact gcgcagggcg aactgaaaac
     6121 cctgacggag atgcagaagc aggaactgct gcgcaatgcg gccctgattg accagcaaaa
     6181 aatccgggag cagttgcggt cccgggaaga gaccctgaag aatgataatg tggctgctcg
     6241 tgcatcaaat gaagcggaac tgctggggta cgggcaggga gagcggctcc gtgaacgcat
     6301 gcgggagttg cagcagatcc gcgacagttt ccgccagaag gatgcggacc ttcagtctca
     6361 gtatcagacc ggggatatca gtgaggattt ttacagacag gctctggcac agaacgcgca
     6421 gtatctgagt gaacgtctga aagagcagga agccttttat gccgaatcgg atgtgcagcg
     6481 tgcggactgg cagaaagggc tgcaggaggg attcagtaac tgggtggata atgcgtccga
     6541 ttacgcctca caggcagcac agcttgcgac ggagggtatc tcagggatgg tgaataacat
     6601 cacggagatg ctgaacggaa ataaagtgga atggcgcagc tgggcctcat cagtgctgca
     6661 ggaaatatca aaagttctta tgaatgccgc gattgtcaac gggatcaaga cggcggcaaa
     6721 cagtatgtcc ggagcgggag gatttatcgg cagtattggt agctggctgg gcggtgcggt
     6781 ggccaatgca aaaggcggtg tgtatacctc ggcaaacctg agtgcgtaca gcaacagtat
     6841 tgtggatacg cccacgtact ttgcctttgc aaaaggggcg ggactgatgg gggaggccgg
     6901 tcctgaagcc attatgcccc tgacccgggc ggcggatggc tcgctgggtg tgcgagcggt
     6961 gggcagtatg aacggcagtg caggtctggt gtattccccg gtctaccata tcgccattca
     7021 gaatgacggg actaatggcc agatagggcc ggaagctgcg ggcagccttg tgcaactgat
     7081 tgaccagcgg gtgcaggcgg tgatgctgtc catgcgacgt gacggaggaa tgctgagtgg
     7141 ctgagataaa aacgctgcat ctggtcccgc gtgaagggat gcaggtgagt gagaaaccgt
     7201 cggtggcgag ggtacggttt ggtgacggtt atgaacagcg ccgcccgacg ggacttaatg
     7261 cccgactgaa gacgtttcag gcggtgttcc gggtgacgga tgaggcgacc cggcgatggc
     7321 tggaagagtt tttatcgtgg catggtggtt accgtgcctt tttgtggcga ccgccgaaac
     7381 ataaccggac ggtgagggtg gtatgccggg agtggagcgt cacagataac gccaggtaca
     7441 gtgatttcag ttgtacgatt gagcaggtgg tgaactgatg caggatattc acgaagaaag
     7501 tctgagcgag tcggttaaat cagagcagtc accgcgggtg gtactctggg aaattgacct
     7561 gacggtgcag ggtggtgagc ggtatttttt ctgtaatgag ctgaatgaaa aaggggagcc
     7621 ggtcacctgg caggggcgta agtatgaggc atacccgatt gacggcagcg gctttgagat
     7681 gaacggccgg ggcagcagtg ccagaccgtc gctgacggtg tccaatctgt tcggtctggt
     7741 caccgggatg gcggaagacc tgcagagtct ggtgggggcc acggtggtcc gccgccgggt
     7801 gtatgcccgt tttctggatg cggtgaattt cgttgcgggc aatccggagg cggacccgga
     7861 gcaggagctg agtgaccgct gggtggtgga gcagatgtcg cagctgacag ccatgacggc
     7921 ctcgtttgtg ctggctacac cgaccgagac ggacggggcg ctgtttcccg gtcgtatcat
     7981 gctggcgaac acctgtatgt ggacctaccg ctctgatgag tgtggttaca cgggcggggc
     8041 tgtggcggat gagttcgata aacccaccac ggatatccgt aaggacagat gcagcaagtg
     8101 catgcgcggg tgtgaactgc gcaggaatgt cggcaatttt ggcggtttcc tttccattaa
     8161 taaactttcg cagtaaatcc cgatttatga cacagactga atcagcgatt ctggcgcatg
     8221 cccggcggtg tgcgcctgcg gagtcgtgcg gcttcgtgat aagcacgccg gagggggagc
     8281 ggtatatccc ttgtgtgaat atttctgcag agccggaggc gtattttcgt atcgcaccgg
     8341 aagactggct gcgggcagag atgcaggggg agattgtggc actggtccac agtcatcccg
     8401 gtgagctgcc ctggctgagc gaggctgacc ggcggctgca gataaaaagc gcactgccct
     8461 ggtggctggt ctgccggggg gaaattcata aattccgctg tgtgccacat ctgacaggac
     8521 ggcgctttga gcacggggtg acggactgtt acacgctgtt ccgggatgca taccatctgg
     8581 cgggaattga tatgccggat tttcatcgcg aggatgactg gtggcgcaac ggccagaacc
     8641 tgtacctgga caatatggcg gtcaccggct tttaccgggt gcccctgtcc tctgcaccgc
     8701 cgggcgatat tctgctgtgc tgctttggtg cttcggtacc gaaccatgcc accatttact
     8761 gcggcaacgg tgagctgctt caccatctgc ctgaacaact gagtaaacgg gagaggtatt
     8821 ccgaaaaatg gcaacgacga acgcattctg tctggcgtca ccgccactgg cacgcatctg
     8881 ccttcacggg gatttgcaac gatttggccg ccgcctcagc ctgtatgtga acacggcagc
     8941 ggaagccatt cgcgccctgt cgatgcagat gccgggcttt cgccgtcaga tgaacgaagg
     9001 ctggtaccag atacgtattg ccggtgatga cacggcaccg gaggcggtgt atgcccgtct
     9061 tcacgaacag ctgggtgagg gaacggtcat ccacattgtg ccgcgactgg ccggggccgg
     9121 aaagggtgga ctgcagattg tgttgggggc ggcagccatc gtgggctctt tcttcactgc
     9181 cggggcatca atggcgttat ggggttcagc cctggcagcc ggtggttttt ctgccaccac
     9241 gatgctgttt tcacttggag ccagcatgat tctgggcggt gtggcccaga tgctggcccc
     9301 gaaggcaaaa acaccggatt accgcgcaac ggataacggc agacagaaca cgtacttttc
     9361 ctcgctggat aacatgattg cccaggggaa cccgatgccg gtgccttacg gggaaatgct
     9421 ggttggctcc cgccgtatat cccaggacat cagcacccgt gatgaaggcg ggggcggaaa
     9481 ggtcgtggtt atcgggcgac agggataaaa cataaaaaaa tcccgcagtg atcgcggagc
     9541 tgcggggaca gacaaatgaa gatcaatgtg aaggagttgt ttttgttact cgggcaaaaa
     9601 aacactaacg cagcgaaatt ataagcgcca cagtcagtgt gtgaaaatgt gaagatattc
     9661 agaaatttta ttccgtcatg acgcaggcac ccggtgaggt gcctgttgtt tttgtgagtg
     9721 aacaattatc acggtaagag
//
LOCUS       DAJTMC010000159         9509 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.159, whole genome shotgun sequence.
ACCESSION   DAJTMC010000159 DAJTMC010000000
VERSION     DAJTMC010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9509
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.159"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            <1..191
                     /locus_tag="OO130_003419"
     CDS             <1..191
                     /locus_tag="OO130_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5366695.1"
                     /translation="AHFMMSGGNILALQKILGHHDIKMTMRYAHLAPDHLETALRFNP
                     LATLPSGDKVAAAVGITP"
     gene            complement(296..832)
                     /gene="rcdA"
                     /locus_tag="OO130_003420"
     CDS             complement(296..832)
                     /gene="rcdA"
                     /locus_tag="OO130_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HCR5366696.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            916..2124
                     /locus_tag="OO130_003421"
     CDS             916..2124
                     /locus_tag="OO130_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5366697.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            2124..2939
                     /gene="ybjI"
                     /locus_tag="OO130_003422"
     CDS             2124..2939
                     /gene="ybjI"
                     /locus_tag="OO130_003422"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HCR5366698.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFTTVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            3025..3309
                     /locus_tag="OO130_003423"
     CDS             3025..3309
                     /locus_tag="OO130_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5366699.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYARHLASVGEGMVIEHQIDDHHH"
     gene            complement(3350..4580)
                     /gene="mdfA"
                     /locus_tag="OO130_003424"
                     /pseudo
     CDS             complement(3350..4580)
                     /gene="mdfA"
                     /locus_tag="OO130_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
     gene            4865..5461
                     /gene="ybjG"
                     /locus_tag="OO130_003425"
     CDS             4865..5461
                     /gene="ybjG"
                     /locus_tag="OO130_003425"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCR5366700.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRFCFALPIRKGWVRD"
     gene            5519..6277
                     /gene="deoR"
                     /locus_tag="OO130_003426"
     CDS             5519..6277
                     /gene="deoR"
                     /locus_tag="OO130_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HCR5366701.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPHSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(6324..7526)
                     /gene="dacC"
                     /locus_tag="OO130_003427"
     CDS             complement(6324..7526)
                     /gene="dacC"
                     /locus_tag="OO130_003427"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HCR5366702.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKDVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGSVTIPRGQLKNLKASY
                     TLTEPQLTAPLKKGQVVGTIDFQLNGKSIEQRPLIVMENVEEGGFFGRMWDFVMMKFH
                     QWFGSWFS"
     gene            7773..8398
                     /gene="gstB"
                     /locus_tag="OO130_003428"
                     /pseudo
     CDS             7773..8398
                     /gene="gstB"
                     /locus_tag="OO130_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
     gene            complement(8395..>9509)
                     /gene="yliI"
                     /locus_tag="OO130_003429"
     CDS             complement(8395..>9509)
                     /gene="yliI"
                     /locus_tag="OO130_003429"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HCR5366703.1"
                     /translation="HRQSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLPD
                     NHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWLS
                     YSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYLF
                     IALGENNQRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGARAEIWSYGIRNPQGM
                     AMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAGT
                     EQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRIL
                     TDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
BASE COUNT         2302 a         2457 c         2346 g         2404 t
ORIGIN      
        1 cggcgcactt catgatgtcg ggtggaaaca ttctggcctt acaaaaaatt ctcggacacc
       61 acgatataaa aatgactatg cgttacgcac atctggcacc ggatcatctg gaaacggcgc
      121 tccgtttcaa tcctctggca acgctgccaa gtggcgacaa agtggcggca gcggttggca
      181 ttaccccgta ataaccacca ctgaccacca acctaactta ttgtttttaa tctaacttat
      241 tgttttcatt aacccgttta cataaatggg ttttttgtta tctaaaattt atctactacc
      301 ctgcaaccct ctcaaccatc ctcaaaatct cctcgcgcga tagcggctta cggtcggtga
      361 caaaatgcag cgtcatcccc tcaataaacg catcaagcgc gcgggcggtt ccgggttcaa
      421 accattgttc gagcgtttgc tgactgcgct gcatccagtt ttgcattacc gtttttaata
      481 gcggttttcg gctagccagc gcgtagagct ggtacatcag ctccatatta tccggcgttg
      541 caacctgtga gctgtagatc atatcggtga tagcctggca tgcgccttga gcatcactaa
      601 catcgctaaa aaatgcctga tattgccggg acataatctc agtaaaactg ctgaacgcct
      661 ccagcaacaa ctcatcaatt cctgaaaaat agtaggtcat cgatcccaac ggtaccccgg
      721 caagggtagc aattttgcgg tgcgtaacag catgtattcc gtaaagtttc accgcctcca
      781 gcgtggcctg gataattttt tctcgccgtt gcggatcgtt agcgcgacgc atgtcatttc
      841 ctctgtctgc aattgtgtac aaatgtacac aaccttgcta atgttgtctt ccctcttctt
      901 attttgacgt gatctatgac cgtaaattct tcacgtaatg cattgaaacg ccgaacctgg
      961 gcgctgttta tgttcttctt tttgccaggc ctgttaatgg cgtcctgggc aacccgtacg
     1021 cctgctatcc gcgatattct ctctgtctcg atcgctgaaa tgggcggtgt tctctttggt
     1081 ctgtcgatcg gttcgatgag cggtattctc tgctcggcgt ggttagtgaa acgctttggg
     1141 acacgtaatg tcatcctggt cacgatgtcc tgcgcattga tcgggatgat gatattaagt
     1201 ctggcactct ggctgacatc gcccctgctc tttgccgttg gtctcggcgt ctttggggca
     1261 agttttggtt ctgcggaagt ggcgataaac gttgaaggtg ccgccgttga gcgagaaatg
     1321 aataaaacgg ttttgccgat gatgcacggt ttttatagcc tgggcacgct ggcaggcgct
     1381 ggtgtcggga tggcactgac ggcctttggc gttccggcaa cggtgcacat tttattggcg
     1441 gcgctggtag gtatcgcgcc tatttatatc gccattcagg caatccctga cggtacgggc
     1501 aaaaatgctg ccgatggcac ccagcatggc gaaaaaggca ttccttttta tcgcgatatc
     1561 cagttgctgt tgattggtgt tgtagtgctg gcgatggcct ttgccgaagg ttctgccaac
     1621 gactggttac ccttattaat ggttgacggt cacggtttta gccctacttc cggctcgctg
     1681 atttatgccg gttttaccct ggggatgacc gttggacgct tcactggcgg ttggttcatc
     1741 gaccgttaca gtcgcgttgc cgtggttcgg gccagtgcac taatgggggc gttgggtatt
     1801 gggctgatta tttttgtcga tagcgcctgg gtcgctgggg tgtctgttgt actctgggga
     1861 ctgggtgcct cgctgggctt cccgctgacc atttctgccg ccagcgatac cggccccgat
     1921 gcaccaaccc gcgtcagtgt ggtagcaacg accggttatc tggctttcct cgtcgggccg
     1981 ccgctgctgg gctatctcgg cgaacattat ggattacgta gtgcaatgct ggttgtactg
     2041 gcgctggtta ttctcgcggc tattgtcgcg aaagccgtcg ccaaacccga taccaaaacg
     2101 cagacggcga tggagaatag ttgatgagca ttaaattaat tgcggtagac atggatggta
     2161 ctttcttaag cgatcaaaaa acctataacc gtgagcggtt tatggctcag tatcagcaaa
     2221 tgaaagcaca aggcattcgc tttgtggtcg ccagcgggaa tcaatattat cagttgatct
     2281 ctttcttccc tgaaattgct aatgaaattg cctttgtggc tgaaaacggc ggctgggtag
     2341 tgagtgaagg caaagatgtt tttaatggcg agctgtcgaa ggatgccttt actactgtcg
     2401 tggaacattt gctgacgcgc ccggaagtgg aaattattgc ctgcggaaaa aatagcgcct
     2461 atacgctcaa aaaatatgac gatgccatga aaacggtggc ggaaatgtat tatcaccgtc
     2521 tggaatacgt tgataacttt gacaacttag aagatatttt ctttaagttt ggcctgaatc
     2581 tttccgatga actgattcca caagtacaaa aagcattaca tgaggccatc ggcgatatta
     2641 tggtgccagt ccacaccggc aacggcagca tcgatctgat tatccccggc gtacataaag
     2701 ccaatggcct tcgccaactg cagaaattat ggggaataga cgacagcgaa gtggtggtct
     2761 ttggcgatgg cggtaacgat attgagatgc tgcgtcaggc aggctttagt tttgcaatgg
     2821 aaaatgccgg cagcgcggtc gtcgcagcgg caaaataccg ggcaggctcc aataaccgtg
     2881 aaggcgtact ggatgtgatc gataaagtgc ttaaacacga agcgccattt gaccaataaa
     2941 aacggcagca cgcccggcaa cacaatacct gaatgaaggc tgcataattc gcggaaatta
     3001 acccgctgca ttatttcagg cgaaatgatt atgaaaaatt gtctactgtt gggcgcgctt
     3061 ttaatgggct ttactggtgt ggcgatggcg caaagtgtca ccgtcgatgt gccgagtgga
     3121 tacaaagtgg ttgtagttcc tgattcagtc agcgttccac aagcggtcag cgtcgcgact
     3181 gtgccgcaaa ccgtctatgt ggctcctgcg ccagctccgg cttaccgccc gcatccttat
     3241 gctcgccatc ttgccagcgt aggtgaaggg atggttatcg aacaccagat tgacgaccat
     3301 caccattaat tcgagaatgc ctgatcgcac aaagcagtca ggcatttttt tacccttcgt
     3361 gagaatttcc catctgttta tcttttaaaa agataaccat cagcgacagc cacaaaattc
     3421 cgttgacaag gttgaagaga ttaaactgtc cgttgccccc gttcagccag gcatgtttgc
     3481 tgatttcaat accaacggta aagatcagca tttgcagcat tcccatcgcc gcagaaaccg
     3541 tacctttact catatcgctg gcaaacaggg ttaatcgcac cagtcccgca ttcgccagac
     3601 caataccgaa agcataaata cttaacccgg cagtcatcca taaatacgcg tgcgatgaga
     3661 taaccgttgc cgcagcagcg accaacagac caatcataat cggccagccg cccataatta
     3721 tcagcgaacg tacggtgcgg cacgaggtca gacgcgctaa cagcaagtta cccgcaatta
     3781 acgccccgaa aataggcact tgcagcaagc catattcata gctgctcaac tgctcgccgg
     3841 taatgatgat aatcggcgac tgggcgatcc acgccagcaa tggcaggcta acgaatccca
     3901 gcgccagcgc ccccgccaca aagcggccgt tcttcagcac cagcttatag tcacgaccga
     3961 gttctttcag tgacagtttc tcgcctatac gcgtggcggt ttcaggcata gctcgttgca
     4021 gaccgaaaaa ggagatcgct gccaatgcgg caaacaaaac aaacatcccc tcccagggca
     4081 gcacatggat ccacgccgcg cccaccagcg gaccaagtag cggagcaatc agcgccacgt
     4141 tcgccatcag cgcggtgatc ttgatacaaa ccgcctcttc gaaagattcc tgaattgcgg
     4201 cgtatcccac agcgccaatg aaacagaggc ttatgccctg caagaagcgc aacagagtga
     4261 attgttcaat gttttgcgcc agtaatattg ccagacaggt gatgataaac cacaccaccc
     4321 ccgccagcat caccggacgg cgaccaatac gatccgacag cggccccagg agccattgta
     4381 aaaacatccc gcccgccaga tatgcggtca ttgaagtagg aacccaatca atgcccgcct
     4441 gatattgttc caccacggcc aacataccgg gttgaatcat atctgccgat ataggttgaa
     4501 aattcgtaaa gcaccagaca gagagggaaa agtaacgcct gacgtccaag cctggcaccg
     4561 gaagctaatt tattttgcat gcaatttctt cgccaataat aatcgcgcag agtttaataa
     4621 aagcgcagct aacgagaaag cgaattttgt agctgaaacc acggttaagc acattcttac
     4681 attattgcga gtatagctac gctttcttta agttttattt aacctatgcc cgttacaatc
     4741 acccaccgta aacaggccgc ttgagggaaa taagacgatg ccgctttacc cagtttaacc
     4801 tgcactttat tctcaacgac ttgcctgtat tggctccctt ttaatcactt tgcgtcggga
     4861 agttatgctg gaaaatttga atctctctct attctctctt attaacgcga cgccagactc
     4921 ggctccgtgg atgatctcgt tggcgatttt tattgctaaa gatttgatta ccgtggtgcc
     4981 gctgctggcc gcggtgcttt ggttgtgggg gcttacagca caacggcaac tggtgataaa
     5041 aatcgctatc gcgctggcgg tcagcctgtt tgtgtcctgg acgatgggac atctttttcc
     5101 gcacgaccga ccctttgtcg aaaatatcgg ctataacttc ctgcatcatg cggcggatga
     5161 ctcattccca agcgaccacg gtacggtgat tttcaccttt gcactggcat ttttatgctg
     5221 gcatcgcctg tggtccggct cacttttaat ggtgctggcc gtcgtcattg cctggtcgcg
     5281 cgtttatctt ggcgtccact ggccgctgga tatgctcggt ggattgctgg caggtatgat
     5341 tggctgcctt agtgcccaga ttatctggca agcgatgggg cataaactct atcaacgtct
     5401 gcaatcgtgg tatcgcttct gttttgcatt accgatccgc aaaggctggg tgcgtgactg
     5461 atctctgcta aaaagtgtag tattgagcgg ctcgcttcaa taactattca gagggattat
     5521 ggaaacacgt cgcgaagagc gtatcgggca gctgctgcaa gaattaaaac gcagcgataa
     5581 gttacatctt aaagacgccg ccgccctgct tggggtttcg gagatgacga ttcgtcgcga
     5641 tctgaacaac cacagtgcgc ccgtcgtttt gctcggcggc tatattgttc tggaaccgca
     5701 tagtgccagc cattacctgt taagcgatca aaaatcccgc ctggtggaag aaaaacgccg
     5761 ggcggcaaaa ctggctgcga cactggtaga acccgatcag accctctttt ttgactgtgg
     5821 caccaccacg ccgtggatta ttgaagcgat tgataatgaa atccctttta ccgccgtttg
     5881 ttattcgcta aatacctttc tggcgctgaa agagaaaccc cattgccgcg catttctgtg
     5941 cggtggtgaa tttcacgcca gcaacgccat tttcaaaccc atcgattttc agcaaacgtt
     6001 gaataatttt tgcccggata tcgcttttta ttccgcggcg ggcgtgcatg tcagtaaagg
     6061 cgctacctgt tttaatcttg aagagttgcc ggtaaaacac tgggccatgt cgatggcgca
     6121 aaagcatgtg ctggttgtcg accacagtaa atttggcaag gtccgtccgg cgcgcatggg
     6181 tgacctgaaa cgctttgata ttgtggtgag tgattgttgc ccggaagatg agtatgtgaa
     6241 gtacgcgcag acgcaccgta ttaagttgat gtattaatga cgtataaccg gatgacgttt
     6301 cgcgccatcc ggttatcaga agattaagag aaccagctgc cgaaccactg atggaatttc
     6361 atcatcacga aatcccacat ccgaccaaag aatccgccct cttccacatt ttccatcacg
     6421 atcagcggac gctgctcaat ggatttaccg ttaagctgga aatcaatggt cccgacaacc
     6481 tgaccttttt tcagcggtgc ggtaagctgc ggttccgtta acgtgtagct cgccttcagg
     6541 tttttcagct gcccacgcgg tatggtcact gagcccgctt cgcctgcccc gagattcact
     6601 tcgctcttat caccaaacca gacgcgctga gtcacaaagg tggcatcagg tttaattggc
     6661 gtcacggttt caaagaagcg gaaaccccag gtcaataatt tctcagactc attaaaacgg
     6721 atacggtcag ttttcgcccc caacactacg gagattaaac gcatatcgcc ctgggtagcc
     6781 gaagcaacca gattatatcc cgcgcctgcc gtggttcctg tcttcatgcc atcaacattc
     6841 agattgctgc tccacagcag acggttacgg ttaggctgac gaattttgtt gaaggtgaac
     6901 tctttctctt tatgaatggc gtactcttcc ggcacatcgt ggatcaatgc tttacccagc
     6961 aatgccatat cgcgcgcggt actgaactgc cccggcgcat ccaggccgtg caccgtctgg
     7021 aaggtagtgt tggtcagacc cagttttttg gcataaccat tcatcagacc aataaatgac
     7081 tcctggctcc cggcaacgta atcggccagc gcaatacagg cgtcattacc ggactggata
     7141 atcacatctt tgttcaagtc tgccaccgaa acctgatcgc ccggtttgag gaacattacc
     7201 gatgaaccac gcagtgccgg atttcccgtc gcccaggcat ctttaccgac cgtcaccata
     7261 tcggtgagtt taatcttatc ggccttaagc gcctgcccaa ccacatagct ggtcatgatt
     7321 ttagtcaggc tcgcgggatc cagtttctca tccgcattgc cttctgccag cactttaccg
     7381 ctggcgtaat ccattaaaat ccatgcacgc gcatccacgc tcggcgcttc aacggtttgt
     7441 tcggccgcga atgccgttgg ggcaaaaagg aataaaaatg cagaacccgc tgcaagacca
     7501 cgaaggagag aggagtattg cgtcataaat gccacccgag tatccattca aaaaaaatac
     7561 gttatatcgc cgtatgataa gttcggtgat taataacgca cacacaaact taaagaaacc
     7621 gtaagttggt aaagttttta aagtttatgc aatatcctgc cgcttcgctg cttctggtgt
     7681 catttttttg ccagctgttg cttaaaagtt aactttactt caccatcgga gtcctgttca
     7741 gcctgtggct gcctgtcgga aaaggggtga gtatgattac gctgtggggt cggaataatt
     7801 caaccaacgt aaaaaagtat tgctgacgct cgaagaactg gaactacctt atgagcaaat
     7861 tctcgcgggc cgtgagtttg ggataaatca cgatgctgat tttctggcga tgaaccctaa
     7921 cgggctggtg ccgttgttgc gtgacgacga aagtgatctc attctttggg aatcaaacgc
     7981 cattgtccgc tatctggcgg cacagtacgg gcaaaaacgc ctgtggatcg actcaccggc
     8041 acgtcgtgcg gaagcggaaa aatggatgga ctgggcaaac cagacgctca gtaatgccca
     8101 tcgagggatc ctgatgggat tagtcagaac accaccggaa gagcgcgatc aggccgccat
     8161 tgatgccagt tgcaaagagt gcgacgccct gtttgccctg ctcgatgcgg aactggcaaa
     8221 agtaaaatgg ttctctggcg acgagtttgg tgtgggcgat atcgctatcg caccgttcat
     8281 ctacaatttg tttaacgttg gcctgacctg gacaccgcgt ccaaatctgc aacgctggta
     8341 tcagcaactc actgaacgcc ccgcagtgcg caaagtggtg atgattcccg ttagctaatt
     8401 gcgtgggcta actttaagta attccccact ggactcgtcg gtgagaacgt ataaataacc
     8461 gtcgggtcca gtgcgaacat cacgaattcg ctgccctctg tccgttaaaa tacggccatc
     8521 ttctgtcact ttgtcgccgt tgacgctcat cacaatgaca tctttatctt tcagcgcgcc
     8581 aataaataat ttttgctgcc actgggggaa tttatcgctg ttatagaagg ccatgccgct
     8641 cacagcgggc gaatctttcc agtaaaaaac aggttgctcg gtcccggcga cgatctcccc
     8701 tttcgcttcc ggtatcttaa agcctgaata gttgattccc caggttgcca gcggccagcc
     8761 gtagtttttg cctttttgcg ggatattaat ttcatcacca ccgcgcgggc catgttcatt
     8821 cagccacagt gcattactcc acggattcat cgccattcct tgcggattac gaatgccata
     8881 agaccagatc tcggcgcgcg caccggattc ctttataaaa ggattatcat ccgggatttc
     8941 gccctgatcg gtcagacgca ccagtttgcc ctgtaattta tccagatcct gcgccgtcgg
     9001 gcgctgattg ttttcgccca gagcaataaa aagataacct ttaccgtcga ataccagccg
     9061 cccgccaaaa tggttgccgg tagacagttt tggcatctgg cggaagacgg tgcggaagtc
     9121 ggtcactttt gagagatcat cacttaagcg gccataaccc acagcagttc cggctttgcc
     9181 atcatcgcca acttcggaat aacttaacca gatgcggcga gactgagcaa aatcaggcgc
     9241 taaaaccacg tccagcaggc cgccctgccc gtgcgcccaa acgtccggaa ctccggaaag
     9301 cggcgcagat aatccttttc ctgcttgcca gtgacgcaac tcgccgccgc gcagagtgat
     9361 taacataccg tgattatcgg gtaaaaaggc cagtgcccag ggatggtcga gtttgtcttg
     9421 cagtacttcg acatttaccg ttgcaggagc cgcccagaga gcggaagaaa gacaaataag
     9481 gggcacaagg aaaaaggatt gtcgatgca
//
LOCUS       DAJTMC010000160         9491 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.160, whole genome shotgun sequence.
ACCESSION   DAJTMC010000160 DAJTMC010000000
VERSION     DAJTMC010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9491
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.160"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            45..728
                     /locus_tag="OO130_003430"
     CDS             45..728
                     /locus_tag="OO130_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HCR5366704.1"
                     /translation="MNLKKIFFSAVTVSVLCALTGCDYIEEGKPESSLLKQQEEHNNK
                     IDLLEKQQAQLKSQLETIQKQQTGIISSTKTLTHVIKSVKDQQNTFIFTEFNPAKTKY
                     FILNNGSVALAGRVLSIDATENGSVIHISLVNLLSTPISNIGFNATWGGEKPVDAKEF
                     ARWQQLLFNTSMKSTLKLLPGQWQDINLTLKGVSPNNLGYLKLAINMENIQFDNLPSA
                     ENRQKRSKK"
     gene            772..5496
                     /gene="ehaG"
                     /locus_tag="OO130_003431"
     CDS             772..5496
                     /gene="ehaG"
                     /locus_tag="OO130_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric autotransporter adhesin EhaG"
                     /protein_id="HCR5366705.1"
                     /translation="MNKIFKVIWNPATGNYTVTSETAKSRGKKSGRSKLLISALVAGG
                     MLSSFGALANAGNDNGQGVDYGSGSAGDGWVAIGKGAKANTFMNTSGSSTAVGYDAIA
                     EGQYSSAIGSKTHAIGGASMAFGVSAISEGDRSIALGASSYSLGQYSMALGRYSKALG
                     KLSIAMGDSSKAEGANAIALGRSSVASGTDSLAFGRQSLASAANAIAIGAETEAAENA
                     TAIGNNAKAKGTNSMAMGFGSLADKVNTIALGNGSQALADNAIAIGQGNKADGVDAIA
                     LGNGSQSRGLNTIALGTASNATGDKSLALGSNSSANGINSVALGADSIADLDNTVSVG
                     NSSLKRKIVNVKNGAIKSDSYDAINGSQLYAISDSVAKRLGGGAAVDVDDGTVTAPTY
                     NLKNGSKNNVGAALAVLDENTLQWDQTKGKYSAAHGTSSPTASVITDVADGTISASSK
                     DAVNGSQLKATNDDVEANTANIATNTSNIATNTANIATNTTNITNLTDSVGDLQADAL
                     LWNETKKAFSAAHGQDTTSKITNVKDADLTADSTDAVNGSQLKTTNDAVATNTTNIAN
                     NTSNIATNTTNISNLTETVTNLGEDALKWDKDNGVFTAAHGTETTSKITNVKDGDLTT
                     GSTDAVNGSQLKTTNDAVATNTTNIATNTTNISNLTETVTNLGEDALKWDKDNGVFTA
                     AHGNNTASKITNILDGTVTATSSDAINGSQLYDLSSNIATYFGGNASVNTDGVFTGPT
                     YKIGETNYYNVGDALAAINSSFSTSLGDALLWDATAGKFSAKHGTNGDASVITDVADG
                     EISDSSSDAVNGSQLHGVSSYVVDALGGGAEVNADGTITAPTYTIANADYDNVGDALN
                     AIDTTLDDALLWDADAGENGAFSAAHGKDKTASVITNVANGAISAASSDAINGSQLYT
                     TNKYIADALDGDAEVNADGTITAPTYTIANAEYNNVGDALDALDDNALLWDETANGGA
                     GAYNASHDGKASIITNVANGSISEDSTDAVNGSQLNATNMMIEQNTQIINQLAGNTDA
                     TYIQENGAGINYVRTNDDGLAFNDASAQGVGATAIGYNSVAKGDSSVAIGQGSYSDVD
                     TGIALGSSSVSSQVIAKGSRDTSITENGVVIGYDTTDGELLGALSIGDDGKYRQIINV
                     ADGSEAHDAVTVRQLQNAIGAVATTPTKYFHANSTEEDSLAVGTDSLAMGAKTIVNGD
                     KGIGIGYGAYVDANALNGIAIGSNAQVIHVNSIAIGNGSTTTRGAQTNYTAYNMDAPQ
                     NSVGEFSVGSADGQRQITNVAAGSADTDAVNVGQLKVTDERVAQNTQSITNLNNQVTN
                     LDTRVTNIENGIGDIVTTGSTKYFKTNTDGVDANAQGKDSVAIGSGSIAAADNSVALG
                     TGSVAEEENTISVGSSTNQRRITNVAAGVNATDAVNVSQLKSSEAGGVRYDTKADGSI
                     DYSNITLGGGNGSTTRISNVSAGVNNNDAVNYAQLKQSVQETKQYTDQRMVEMDNKLS
                     KTESKLSGGIASAMAMTGLPQAYTPGASMASIGGGTYNGESAVALGVSMVSANGRWVY
                     KLQGSTNSQGEYSAALGAGIQW"
     gene            5864..7519
                     /gene="lldP"
                     /locus_tag="OO130_003432"
     CDS             5864..7519
                     /gene="lldP"
                     /locus_tag="OO130_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HCR5366706.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            7519..8295
                     /gene="lldR"
                     /locus_tag="OO130_003433"
     CDS             7519..8295
                     /gene="lldR"
                     /locus_tag="OO130_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HCR5366707.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            8292..9482
                     /gene="lldD"
                     /locus_tag="OO130_003434"
     CDS             8292..9482
                     /gene="lldD"
                     /locus_tag="OO130_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HCR5366708.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADTHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPAALAPMAK
                     GNAA"
BASE COUNT         2418 a         2379 c         2457 g         2237 t
ORIGIN      
        1 tctaccggca ttgatcgctg gtcggaggaa ataaggattt ttacatgaac ttgaagaaga
       61 tttttttcag tgctgtcact gtttcagttc tctgcgcatt aacgggatgt gactacattg
      121 aagaaggcaa gccagaaagt agtttattga aacaacaaga agaacataac aataaaattg
      181 atctacttga aaaacaacaa gcacagctaa agagtcaact ggaaactatt caaaaacaac
      241 aaactggcat aataagcagt acaaagactt taactcacgt aattaaatcc gttaaagatc
      301 aacaaaacac ttttattttt accgagttta acccagcaaa aacgaagtat ttcatcctga
      361 acaatggttc ggtggcttta gcgggtcgag tgttatccat tgacgccaca gagaatggca
      421 gtgttattca tatttcactg gtcaacttat taagtacacc tatctcaaat attggtttta
      481 atgcgacatg gggtggtgaa aaacctgtcg atgccaaaga gtttgcccgg tggcaacaat
      541 tacttttcaa cacatcaatg aaatccacat tgaaattatt accaggtcaa tggcaagaca
      601 ttaatttgac cctgaagggt gtctcgccca ataacctggg atatctgaag ttagccatca
      661 acatggaaaa tattcagttc gacaatcttc cctctgctga aaatcggcag aaaagaagca
      721 aaaaataaat gcagttagta cacttataac aaggaatagc gagcatcatt aatgaacaaa
      781 atatttaaag ttatctggaa ccctgcgaca gggaattata ctgttaccag cgaaacggca
      841 aaaagccgtg gcaagaaatc tgggcgcagt aagctgttaa tttctgcgct ggttgcgggt
      901 ggaatgttgt cgtcgtttgg ggcattggcg aatgccggga atgacaacgg tcagggtgtt
      961 gattacggta gtggatcagc tggcgacggc tgggttgcta taggcaaagg ggcgaaagca
     1021 aatactttta tgaacaccag tggttccagt actgctgtgg gttatgacgc tatagctgaa
     1081 ggccaatata gctctgccat cgggtcaaaa acccatgcga ttggtggtgc atcaatggcc
     1141 tttggggtta gtgcaatatc agaaggcgat agaagtatag cactgggtgc ctcttcgtat
     1201 tcattgggcc aatactcaat ggccctcggc cgttattcaa aagcattggg taaattgtct
     1261 attgctatgg gggactcttc caaagcggaa ggagcaaacg ccattgccct ggggcgtagc
     1321 agtgtagcta gcggtactga cagcctcgca tttggcagac aatcacttgc cagcgcagcg
     1381 aacgctattg cgataggtgc tgagaccgaa gccgctgaaa atgcaactgc tattggcaat
     1441 aatgcgaagg caaaagggac taatagcatg gcaatggggt tcggaagcct tgccgataaa
     1501 gtcaatacta tcgcattagg aaatggcagc caggctctgg cagataatgc aatcgccata
     1561 ggccagggca acaaagctga tggcgtggat gccatcgctc tgggtaatgg tagccagtcg
     1621 agaggcttaa acaccattgc cttaggcaca gccagtaatg caactggtga taagagtctt
     1681 gcgcttggta gtaatagcag tgccaacggt attaactctg tcgcgctggg cgcagattcc
     1741 attgcggatt tagacaatac cgtctctgtc ggcaatagtt cattaaaacg caagatcgtt
     1801 aatgtgaaaa atggcgcgat caagtctgac agttacgatg ccattaatgg ttcacaactt
     1861 tatgccatta gcgactcggt agcaaaaagg cttggaggag gggctgcagt agatgttgat
     1921 gacggtactg ttacagcacc aacctacaat ttaaaaaatg gtagcaaaaa taacgtaggg
     1981 gctgcgctcg ctgtacttga tgaaaacacc ctgcaatggg accaaaccaa aggcaaatac
     2041 agcgctgctc atggtactag tagcccaact gccagcgtaa tcaccgatgt tgcggatggc
     2101 acgatttcag cctccagtaa ggatgcggtt aacggttccc aactgaaagc taccaatgac
     2161 gatgtcgaag ccaacaccgc caatatcgct actaatacca gcaacattgc cacgaatacg
     2221 gcaaatattg ccaccaatac caccaatatc accaacctga cggattccgt tggtgacctt
     2281 caggctgatg ccctgctctg gaacgaaact aaaaaggcat tcagtgcagc tcacggccag
     2341 gataccacca gcaaaatcac caacgttaaa gatgccgacc tgacggctga cagcactgat
     2401 gctgttaacg gctctcagct gaaaaccacc aacgatgctg tggcgacgaa taccaccaat
     2461 atcgccaata acacttccaa tattgccact aacaccacca acatctctaa tctgactgag
     2521 acggtgacta atcttggtga ggatgcgctg aaatgggata aggacaatgg tgtattcacg
     2581 gcagctcatg gcaccgagac caccagcaaa atcaccaacg ttaaagatgg cgacctgacg
     2641 actggcagca ccgatgccgt taacggctct cagctgaaaa ccaccaacga tgccgtggcg
     2701 acgaatacca ccaatatcgc cactaacacc accaacatct ctaatctgac tgagacggtg
     2761 actaatcttg gtgaggatgc gctgaaatgg gataaggaca atggtgtctt cactgcagct
     2821 catggcaaca ataccgccag caaaatcacc aatatcctgg acggcacagt cactgcaacc
     2881 agttccgatg ccattaacgg tagccagctt tatgacttaa gcagcaatat cgccacctac
     2941 ttcggcggca atgcttctgt gaatactgac ggtgtgttta ccggtccaac ctacaaaatc
     3001 ggtgaaacaa attattataa cgtcggcgat gcactggctg cgattaactc ctcatttagc
     3061 acgtctctcg gcgatgctct gctttgggat gccaccgcag gtaaattcag tgccaaacac
     3121 ggtactaatg gtgacgcaag cgtgatcact gatgtcgcag atggtgaaat ttcagactcc
     3181 agttctgacg cagtaaacgg ctcacaactc cacggcgtga gcagttatgt tgttgatgcg
     3241 ctggggggtg gtgccgaagt caatgcagac ggcaccatca ctgcgccgac gtacaccatt
     3301 gctaatgctg attacgataa tgtcggtgat gccctgaatg ctatcgatac cactcttgac
     3361 gacgctctgc tctgggatgc ggacgccggt gaaaatggtg catttagcgc cgctcacgga
     3421 aaagataaaa ctgccagtgt aatcactaac gtcgctaacg gtgcaatctc tgctgccagc
     3481 agcgacgcga ttaacggctc acaactctat accaccaata agtacatcgc tgatgcgctg
     3541 gatggtgacg cagaagtcaa cgctgacggc accatcaccg caccgactta caccattgcg
     3601 aacgccgagt acaacaacgt cggtgacgct ctggatgcgc ttgatgataa cgccctgctg
     3661 tgggatgaga ctgccaatgg cggtgctgga gcctacaatg ccagccatga cggtaaagcc
     3721 agcatcatca ctaatgtcgc taatggcagt attagtgagg acagtaccga tgcagtgaac
     3781 ggttctcagt tgaatgcgac gaatatgatg attgagcaga acacccaaat tatcaatcag
     3841 ctcgctggta acaccgacgc aacctatatc caagaaaacg gtgcgggtat taactatgtg
     3901 cgtactaacg acgacggctt agcgttcaac gacgccagcg cacagggtgt tggcgctaca
     3961 gctataggtt ataactctgt cgccaaaggc gatagcagcg tagctattgg tcagggcagc
     4021 tacagcgacg ttgatacggg tatcgcccta ggtagcagct ctgtttccag ccaagtgatt
     4081 gccaaaggct cccgtgacac cagcataacg gaaaatggcg ttgttattgg ttacgacacc
     4141 acggatggcg aactgctcgg tgcattgtct atcggtgatg acggtaaata tcgtcaaatc
     4201 atcaacgtag ccgatggttc cgaagcccat gacgccgtta cggttcgtca attgcagaat
     4261 gcgattggtg cggtcgcaac cacgccgact aaatacttcc acgctaattc aacggaagaa
     4321 gattcactgg cagtgggaac tgactcgctg gcaatgggtg cgaaaaccat cgtgaatggc
     4381 gataaaggta ttggtatcgg ttatggtgcc tacgtggacg cgaatgcact taacggcatt
     4441 gccattggta gcaatgcgca agtcattcat gtcaacagta ttgcgatagg taatggttct
     4501 acgaccactc gtggcgctca aaccaattat accgcctaca acatggacgc accgcagaac
     4561 tctgtcggtg aattctcagt cggtagtgcg gatggtcaac gtcagatcac aaacgtcgca
     4621 gctggttcag cggataccga tgcggttaac gtgggtcagt tgaaagtcac tgatgagcgc
     4681 gtagcgcaaa atacccagag cattactaac ctgaacaatc aggtcactaa tctggatact
     4741 cgcgttacta atatcgaaaa cggtattggc gacattgtca ccaccggtag caccaagtac
     4801 ttcaagacca acaccgatgg cgtagatgcc aacgcccagg gtaaagatag cgttgctatt
     4861 ggttctggtt ccattgctgc cgctgacaac agcgtcgcac tgggtaccgg ttccgttgca
     4921 gaggaagaaa atacaatctc tgtaggttct tctactaacc aacgccgtat caccaacgta
     4981 gctgccggtg ttaatgccac cgatgcggtt aacgtgtcgc agctgaaatc ttctgaagca
     5041 ggcggcgtgc gctacgacac caaagctgat ggttctatcg actatagcaa tatcaccctc
     5101 ggtggtggca acggcagtac gactcgtatc agcaacgtct ccgctggcgt caacaacaac
     5161 gacgcggtga actacgcgca gttgaagcaa agcgtgcagg aaacgaagca atacaccgat
     5221 cagcggatgg ttgagatgga taacaaactg tctaaaactg aaagcaagtt gagcggtggt
     5281 atcgcttctg caatggcaat gaccggtctg ccgcaggctt atacaccggg tgccagcatg
     5341 gcttctattg gtggcggtac ttacaacggt gaatcggcag ttgctttagg tgtatcgatg
     5401 gtgagcgcca atggtcgttg ggtctacaaa ttacaaggta gtaccaatag ccagggtgaa
     5461 tactccgccg cactcggtgc cggtattcag tggtaatgat ccattaagtt agtgtgacta
     5521 agggcaaacg ccaaattgcc tgatgcgtta cgcgcatcag gcaagataag gcgctccggg
     5581 tcaacaatct aagggcaatt ctctgatgag gattgccctt ttctttacca gacatctccc
     5641 cccacaagaa ttggccctac caattcttcg cttatctgac ctctggttca caattcccca
     5701 attaaaactc acatcaatat tgccaataca caacatttag ttaaccattc attgtcatta
     5761 tccctacaca acacaattgg cagtgccact tttacacaac gtgtgacaag gagatgagca
     5821 acagactcat tacacgatgt gcgtggactc caggagacct gcaatgaatc tctggcaaca
     5881 aaactacgat cccgccggga atatctggct ttccagtctg atagcatcgc ttcccatcct
     5941 gtttttcttc tttgcgctga ttaagctcaa actgaaagga tacgtcgccg cctcatggac
     6001 ggtagcaatc gcccttgccg tggctttgct gttctataaa atgccggtcg ctaacgcgct
     6061 ggcctcggtg gtttatggct tcttctatgg tttgtggccc atcgcgtgga tcattattgc
     6121 agcagtgttc gtctataaga tctcggtgaa aaccgggcag tttgacatca ttcgctcgtc
     6181 tattctttcg ataacccctg accagcgtct gcaaatgctg atcgtcggtt tctgtttcgg
     6241 cgctttcctt gaaggagccg caggctttgg cgcaccggta gcaattaccg ccgcattgct
     6301 ggtcggcctg ggttttaaac cgctgtacgc cgccggactg tgcctgattg ttaacaccgc
     6361 gccagtggca tttggcgcga tgggcattcc aatcctggtt gccggacaag taacaggcat
     6421 cgacagtttt gagattggcc agatggtggg gcgtcagcta ccgtttatga ccattatcgt
     6481 gctgttctgg atcatggcga ttatggacgg ctggcgcggt atcaaagaga cgtggcctgc
     6541 ggtcgtggtt gcgggcggct cgtttgccat cgctcagtac cttagctcca acttcattgg
     6601 gccggagctg ccggacatta tctcttcgct ggtatcactg ctctgcctga cgctgttcct
     6661 caaacgctgg caaccagttc gcgtattccg cttcggtgac ttgggggcgt cacaggttga
     6721 tatgacgctg gcccacaccg gttacaccgc gggtcaggtg ttacgtgcct ggacaccgtt
     6781 cctgttcctg accgctaccg taacactgtg gagcatcccg ccgtttaaag ccctgttcgc
     6841 atcgggtggt gcgctgtatg agtgggtgat caacattccg gtgccgtacc tcgataaact
     6901 ggttgcccgt atgccaccag tggtcagcga ggctacagcc tatgccgccg tgtttaagtt
     6961 tgactggttc tctgccaccg gcaccgccat tctgtttgct gcactgctct cgattgtctg
     7021 gctgaagatg aaaccgtctg acgctatcag caccttcggc agcacgctga aagaactggc
     7081 tctacccatc tactccatcg gcatggtgct ggcgttcgcc tttatctcga actattccgg
     7141 actgtcatca acactggcac tggcactggc gcacaccggt catgcattca ccttcttctc
     7201 gccgttcctc ggctggctgg gggtattcct gaccgggtcg gatacctcat ctaacgccct
     7261 gttcgccgcg ctgcaagcca ccgcagcaca acaaattggc gtctctgatc tgttgctggt
     7321 tgccgccaat accaccggtg gcgtcaccgg taagatgatc tccccgcaat ctatcgctat
     7381 cgcctgtgcg gcggtaggcc tggtgggcaa agagtctgat ttgttccgct ttactgtcaa
     7441 acacagcctg atcttcacct gtatggtggg cgtgatcacc acgcttcagg cttatgtctt
     7501 aacgtggatg attccttaat gattgtttta cccagacgcc tgtcagacga ggttgccgat
     7561 cgtgtgcggg cgctgattga tgaaaaaaac ctggaagcgg gcatgaagtt gcccgctgag
     7621 cgccaactgg cgatgcaact cggcgtatca cgtaattcac tacgcgaggc gctggcaaaa
     7681 ctagtgagcg aaggcgtgct gctcagtcga cgcggcggcg ggacgtttat tcgctggcgt
     7741 catgacacat ggtcggagca aaacatcgtc cagccgctaa aaacgctgat ggccgatgat
     7801 ccggattaca gtttcgatat tctggaagcc cgctacgcca ttgaagccag caccgcatgg
     7861 catgcggcaa tgcgcgccac acctggcgac aaagaaaaga ttcagctttg ctttgaagca
     7921 acgctaagtg aagacccgga tatcgcctca caagcggacg ttcgttttca tctggcgatt
     7981 gccgaagcct cacataacat cgtgctgctg caaaccatgc gcggtttctt cgatgtcctg
     8041 caatcctcag tgaagcatag ccgtcagcgg atgtatctgg tgccaccggt tttttcacaa
     8101 ctgaccgaac aacatcaggc tgtcattgac gccatttttg ctggtgatgc cgacggggcg
     8161 cgtaaagcaa tgatggcgca ccttagtttt gttcacacca ccatgaaacg attcgatgaa
     8221 gatcaggctc gccacgcacg gattacccgc ctgcccggtg agcataatga gcattcgagg
     8281 gagaaaaacg catgattatt tccgcagcca gcgattatcg cgccgcagcg caacgcattc
     8341 tgccgccgtt cctgttccac tatatggatg gtggtgcata ttctgaatac acgctgcgcc
     8401 gcaacgtgga agatttgtca gaagtggcac tgcgccagcg tattctgaaa aacatgtccg
     8461 acttaagcct ggaaacgacg ctgtttaatg agaaattgtc gatgccggta gcactggcac
     8521 cggtgggttt gtgtggcatg tatgcgcgtc gtggcgaagt tcaggcagcc aaagcggcgg
     8581 acacgcatgg cattccgttt actctctcga cggtttccgt ttgcccgatt gaagaagtcg
     8641 cgccagccat caagcgccca atgtggttcc agctttatgt actgcgcgat cgcggcttta
     8701 tgcgtaacgc gctggagcga gcaaaagcag cgggttgttc gacgctggtt ttcaccgtgg
     8761 atatgccgac accgggcgca cgctaccgtg atgcgcattc aggtatgagc ggcccaaatg
     8821 cggcaatgcg ccgctacttg caggcggtga cgcatccgca atgggcgtgg gatgtgggcc
     8881 tgaacggtcg tccacatgat ttaggtaata tctcagctta tctcggcaaa ccaaccggac
     8941 tggaagatta catcggctgg ctggggaata acttcgatcc gtccatctca tggaaagacc
     9001 ttgagtggat ccgcgatttc tgggatggcc cgatggtgat caaagggatc ctcgatccgg
     9061 aagatgcgcg cgatgcagta cgttttggtg ctgatggaat tgtggtttct aaccacggtg
     9121 gccgccagct ggacggtgta ctctcttccg ctcgtgcgtt gcccgctatt gcagatgcgg
     9181 tgaaaggtga tatcgccatt ctggcggata gcggaattcg taacgggctt gatgtcgtgc
     9241 gtatgattgc gctcggtgcc gacaccgtac tgctgggtcg tgctttcctg tatgcgctgg
     9301 caacagcggg ccaggcgggt gtagctaacc tgctaaatct gatcgaaaaa gagatgaaag
     9361 tggcgatgac gctgactggc gcgaaatcga tcagtgaaat tacgcaagat tcgctggtgc
     9421 aagggctggg taaagagttg cctgcggcac tggctcccat ggcgaaaggg aatgcggcat
     9481 agtcgtttgc g
//
LOCUS       DAJTMC010000161         9393 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.161, whole genome shotgun sequence.
ACCESSION   DAJTMC010000161 DAJTMC010000000
VERSION     DAJTMC010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9393
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.161"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(312..890)
                     /gene="mocA"
                     /locus_tag="OO130_003435"
     CDS             complement(312..890)
                     /gene="mocA"
                     /locus_tag="OO130_003435"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HCR5366709.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMLPWQQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYVNQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAIKRPNITNMRQALLMG
                     AHYSVEVENAEIILDIDTPDDFITVQKRYTKI"
     gene            993..1763
                     /gene="yqeC"
                     /locus_tag="OO130_003436"
     CDS             993..1763
                     /gene="yqeC"
                     /locus_tag="OO130_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium-dependent hydroxylase
                     accessory protein YqeC"
                     /protein_id="HCR5366710.1"
                     /translation="MKSIVDPSALFIDLGAQKRPTVISVVGAGGKTSLLFWLAELLQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASLTSPISFCFHSWKANQGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRAMPLKAPDEHEPCIPKSSCCVIAVMGGHILGAKV
                     STENVHRWSQFADITGLTPDATLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHDVEAIWLGDIQEHPAIARRFVN"
     gene            1811..3436
                     /locus_tag="OO130_003437"
     CDS             1811..3436
                     /locus_tag="OO130_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF2563 family selenium-dependent molybdenum
                     hydroxylase system protein"
                     /protein_id="HCR5366711.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHINRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EALDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILQVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSSA
                     EAMKLTERGFIPVMVDPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFT
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTGVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(3477..4409)
                     /gene="arcC"
                     /locus_tag="OO130_003438"
                     /pseudo
     CDS             complement(3477..4409)
                     /gene="arcC"
                     /locus_tag="OO130_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417350.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
     gene            complement(4457..5842)
                     /gene="hyuA"
                     /locus_tag="OO130_003439"
     CDS             complement(4457..5842)
                     /gene="hyuA"
                     /locus_tag="OO130_003439"
                     /EC_number="3.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
                     /protein_id="HCR5366712.1"
                     /translation="MRVLIKNGTVVNADGQAKQDLLIESGIVRQLGNNISPQLPYEEI
                     DATGCYVFPGGVDVHTHFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCR
                     LRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKL
                     NDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAE
                     AIARMINLAQIAGNAPLYIVHLSNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYD
                     TEDGMKFILSPPLRNVREQDKLWCGISDGAIDVVATDHCTFSMAQRLQISKGDFSRCP
                     NGLPGVENRMQLLFSSGVMTGRITPERFVELTSAMPARLFGLWPQKGLLAPGSDGDVV
                     IIDPRQSQQIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRF
                     LRRKPFVPPVL"
     gene            complement(5895..7106)
                     /locus_tag="OO130_003440"
     CDS             complement(5895..7106)
                     /locus_tag="OO130_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HCR5366713.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQIYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            complement(7164..8360)
                     /gene="dpaL"
                     /locus_tag="OO130_003441"
     CDS             complement(7164..8360)
                     /gene="dpaL"
                     /locus_tag="OO130_003441"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HCR5366714.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYRP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQRQSLMEKLALNKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            complement(8418..>9393)
                     /gene="ygeW"
                     /locus_tag="OO130_003442"
     CDS             complement(8418..>9393)
                     /gene="ygeW"
                     /locus_tag="OO130_003442"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HCR5366715.1"
                     /translation="RTRFSYASALNLLGLAQQDLDEGKSQIAHGETVRETANMISFCA
                     DAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQCDIDHPTQSMADLA
                     WLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFGMDVTLAHPEGYDL
                     IPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKVMEERTELLRANDH
                     EGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADISGVSCKEGEVTEG
                     VFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQERVK"
BASE COUNT         2195 a         2291 c         2461 g         2446 t
ORIGIN      
        1 ttagcttata cagatatcat tgagtgaccg gtgttgagaa atccggtggt ttagggggat
       61 agagcaacaa tcgcaccact cttgccaggt ttggcgggaa atataaaaaa atgtgaggcg
      121 gctcgaaact taagggcttt ttgtgaatga actcacaggt gctaaatgag aattaaaatc
      181 actcctgaaa attattattg ccagcccata tatcactttt tctcttttct caaaatcagt
      241 gattccttat cattatgata aagagtgctg gagggattaa atatttgaac gcaatcgttt
      301 aactacgttt tttaaatttt agtatacctt ttttgcactg taataaagtc gtccggagta
      361 tcaatatcta aaattatttc tgcattttct acttcgacgg aataatgagc tcccataagg
      421 agtgcctgac gcatattggt gatattgggg cgcttgattg cctgcatcag gcatggtttt
      481 gacactaaaa tcggatggcc ggggatgcca ttatggagtg gcagtattgc gccatcattt
      541 cgtaacgacc agatttttct gaaaatatcg atggtgaggg ttggcatgtc accgtgggtg
      601 agaaaacaat gttctgtttg cactgcgggt actgcggcct ttactgatgt tagtaaaccc
      661 tgcgcataat ctgggttgtg gataatagtg atattgctct ggttcacata acgttcgtgc
      721 agttcattac cacgatagcc ggtgactaaa ataattcggc tacaaaactg caacgcattt
      781 ttgatacttg tatcaagaat tgtcccctgt tgccagggta acatcatttt ccattgcccc
      841 attcttgatg ataatccggc agcggtaatt atacagtcga tggctgacat agtggttcca
      901 ttatttatag gggctaataa cgcctgataa ttgcgttact tcctgatgga ttaagttggg
      961 gataatttat cccagagagg tcataaagac tcgtgaaaag tatagttgac ccatcggcat
     1021 tattcattga tttaggtgcg cagaaacgtc ccactgtgat ttctgttgtt ggtgctgggg
     1081 ggaaaaccag cttgcttttt tggctggcag aactgctcca ggctagcggc aggcgtgtgt
     1141 taatcacgac gacaacacat atgtttatgc caacgtctca ctggcccgtg gttttctgtc
     1201 gtgatcccgc catgcttcct catgcgtctc ttacatcccc catttcattt tgttttcaca
     1261 gctggaaagc gaaccaggga aaagtgcagg gatttacgcc agaagcgatt gatgcactgg
     1321 tgcaacgacc agagtgtgac gtaattctca ttgaagcaga tggctcgcgt gcaatgccgt
     1381 taaaagcgcc tgatgagcac gaaccttgca tacccaaaag cagttgctgc gtgattgctg
     1441 tgatgggagg acatattttg ggcgcgaaag tgagcacaga aaatgtccat cgctggtcgc
     1501 agtttgctga tattactggg ttaacacctg atgcaacctt gcaactgagc gatctcgttg
     1561 cgctggttcg ccaccctcag ggggcgttta aaaacgtacc gcaaggttgt cggcgagtct
     1621 ggttcattaa ccgtttttct caatgtgaga atgcgattgc acaaagcgag ctccttcaac
     1681 cgctgcaaca acacgacgta gaggcaatct ggctaggcga tatacaagaa catcctgcaa
     1741 tcgcgcgcag atttgtgaat tagcggcccg ggtattccgg gttactgaaa ggatatccat
     1801 tgaggttggt atgaatattt tcacagaggc tgcaaaactc gaagagcaaa attgtccgtt
     1861 tgcgatggcg caaattgttg atagccgagg ctcgactccc cgccattctg cacaaatgtt
     1921 agtgcgcgcc gatggttcta tcgtcggtac aattggtggc ggaatggttg agcggaaggt
     1981 gattgaagag tcacttcagg cattgcagga acgtaagccg cgattattcc atggacgtat
     2041 ggctcgtaac ggtgcggatg ctgtcgggtc agattgtgga ggtgcaatgt cagtatttat
     2101 cagcgtccat ggtatgcgtc cacgtctggt gttgattggc gcggggcata tcaaccgggc
     2161 gatagcccag agtgccgcgc tattaggatt tgatattgcc gttgccgata tttatcgcga
     2221 aagcctcaat cctgaactat tcccaccatc aaccacgctt ctccatgctg agtcgtttgg
     2281 tgcggcagtg gaagcactgg atattcgccc tgataatttt gtcctgattg ccacgaataa
     2341 tcaggatcgt gaagccctcg acaaactcat tgaacagccc attgcatggt tggggttgct
     2401 ggcaagtcgc cgcaaggttc agcttttcct gcgtcaattg cgtgagaaag gcgtggctga
     2461 agaacatatt gcccgtttac atgcgcccgt tggttacaac ataggtgcgg aaacgccgca
     2521 ggagatcgcc atcagcgtgc tggcagaaat attacaggtg aaaaataacg cgccgggtgg
     2581 gctgatgatg aaaccttctc atccttccgg acaccagctg gtggtgattc gcggtgcggg
     2641 agatatcgcc agtggtgtgg cgctacgtct gtatcatgcg ggttttaaag tgatcatgct
     2701 ggaagtggaa aaaccgacag tgattcgttg taccgtggcg tttgcccagg ccgtgttcga
     2761 tggcgaaatg acggtcgaag gcgtcactgc tcgcctggca accagctctg cggaagcgat
     2821 gaaacttacc gaacgcggat tcatccctgt gatggtagat cccgcctgtt cattgcttga
     2881 tgaactgaaa ccgctttgcg tggtggacgc tattctggcg aaacagaatt tgggaacgcg
     2941 ggcagatatg gcaccagtaa caatcgcgct tgggccgggc tttactgcag ggaaggattg
     3001 tcatgcggta attgaaacaa atcgcgggca ctggctcggt caggtgattt actctggttg
     3061 tgcgcaggag aataccggtg ttcctggcaa tattatgggg cataccaccc gacgggtgat
     3121 ccgtgctcct gctgcaggca ttatgcgatc caacgtgaaa ttaggcgatc tggtgaaaga
     3181 gggcgatgtg attgcctgga ttggtgagca tgaaattaaa gcaccgttga cggggatggt
     3241 gcgtggcttg ttgaacgacg gcctggcagt ggttggtggt tttaaaattg gtgatatcga
     3301 tcctcgtggt gaaaccgctg atttcaccag cgtttctgat aaagcccggg cgattggcgg
     3361 cggcgtactt gaggcgttaa tgatgttgat gcatcagggc gtgaaagcga caaaagaagt
     3421 gctggaagtg gcttaaatga aaatggccgg atggtattac ccacccggcc ttaaaattac
     3481 tgacaaatca ccgttccggt tttgccttca atcccttctt tcgctttgct caatacggta
     3541 atcagtgctt cacgacctgc gcgagagcga gcaaacgagg cggcagcttc cactttaggc
     3601 agcatagaac ctttcgcaaa atgaccttct tcgataaagc gttcagcatc acttaatgac
     3661 aggcgatcga gccactgttc attctcttta ccgaagttaa tggccacttt ttctaccgca
     3721 gtcagaatga tcagcatatc ggcatcaatc atttctgcta aacgggcgct ggcccagtct
     3781 ttatcgataa ccgcgctggc accacgcaga tggttacctt cacgaataac agggatacca
     3841 ccaccgccaa cggtgatcac cacctggcct gcatctacca gagctttaac agtttctttt
     3901 tcaatgatat caaccggttt tggcgaggca acaacacggc gatagccgcg accagcatct
     3961 tctttcagag tgtaaccctg ttttgtcagt tgctctgctt cttgctcggt aaagaacgaa
     4021 ccgatcggct tggtcgggtt gaggaatgcc ggatcgttag cgtcgacttc gacctgagta
     4081 accagcgttg ctacaggttt attaatgccc cgagaaagca gttcttctct taacacgttt
     4141 tgtagatcgt aaccaatata accctagctt aaggcaacac agacagacat cggcagcatt
     4201 ggcgagtgtg cttcagtttt tgcggcagct tcaaaggcct ggttaatcat gcccacctgc
     4261 ggcccgttac catgagtgac gataacttca tgtccttggg caattaaatc aacaattgcc
     4321 tgagaagtaa tttttaccgc tttcatttgt ccggccaggt catcgcccag cgcattcccg
     4381 cccagggcga gaacaatttt cttactcatt tttcctcact catcattctt gattgtgtat
     4441 agatccctga cagtggttag agcacgggag ggacaaacgg tttgcgtcgc aggaaacgac
     4501 ctcgcccggc tttgcctgta aaggtgccgt cacagaaaat cgtttcacca cgggataagg
     4561 ttctgacaat cgcgccctga caggtaaaac cctcccatgg cgagtagtcg gcgttgtcgt
     4621 ggagatggcg atgctgaatt tgttggctct gacgtgggtc gataatcacc acgtcgccat
     4681 cggaaccggg cgctaataat cctttttgcg gccacaggcc aaacagcctg gcgggcattg
     4741 cgctggttaa ttcaacaaag cgttccggtg ttatacgtcc cgtcatcacg ccactggaaa
     4801 acagtaactg catgcggttt tccacaccgg gtaagccatt tgggcagcga ctgaaatcgc
     4861 ctttagaaat ttgcaggcgt tgagccatcg agaaggtgca gtgatcggtc gccaccacgt
     4921 caatcgcacc atcgctgatg ccacaccaca gtttgtcctg ctcgcgtacg ttacgcagcg
     4981 gtgggctaag aatgaacttc atgccatctt ctgtatcgta actgcgttcg tccaacagga
     5041 gatattgtgg gcaggtttca acccagactg gctggtgatt cgcacgggca agacgcagat
     5101 aatccagacc taagccgtta gacaggtgca cgatatagag cggggcgtta ccggcaattt
     5161 gtgccaggtt aatcatgcgg gcgatggctt ccgcttcgca ttccagaggg cgactcaagg
     5221 catgatagcg cggcgcggtt aaccctgcgg cgataaattc cgcccgcttg ctggcgatag
     5281 ccgcatcatt ttccgggtgc acggtggtca gcgcgccgga ttcatgcaag cggcgtaatg
     5341 cctgcaaaac ctcgtcatcg ttgagtttgt attgataggt taaatagagt ttaaaactgc
     5401 tcagtccttc ctcgaccatc atcgggattt cgtcgaggat tgcgtgatta atgtgctgga
     5461 tcacaccgtg aaagctgtaa tcgatgaccg ctttatgggc ggcataacca cgataaacct
     5521 ccagttgatg gcgtaaccga cagccgtttg ggccaaatcc catatggtca ataatggttg
     5581 ttgtaccgcc acacgcagct gcgcgggtac cggtaaaaaa atcatcacaa ctgcgcgcga
     5641 tgccgacatc aatattgaaa tgcgtatgga catccacgcc gcctgggaaa acgtaacagc
     5701 cagtggcatc aatttcttca tacgggagct gcggcgaaat attgttgccc aactggcgaa
     5761 caatcccgct ttcaatcagc aaatcctgtt tggcttgtcc atctgcgtta acgacagtgc
     5821 cgtttttgat caatacgcgc atagcaaact ccaaaaaccc tccggcgaac cggaggggaa
     5881 taagtgaaga gtaattactc tgttgccagc cagcttaacg ggattgcagc gtacatcgcg
     5941 gcacaggtca ccaggtgaga tttccaggtt ttttcgttag gtgcatgcgc ttcaggttct
     6001 ttacccgggc caaagccgat caccggaatg ccgtgacggc ccatgataga tacgccgtta
     6061 gttgagaagg tccacttatc aacaaccggc gctttgccaa acagcccttc gtaggcattc
     6121 accagtgctt taacggtgaa gtgatcttct tccactttcc aggtcgggaa gtagcattcg
     6181 gttgggtaaa ccaggccagt ccaggacgga cggtcgtagt tgtacataga aacaaccgcg
     6241 ttagctttct gtactgcagg cagggcgcgg atttcgtcca gcgcgccttc ccaggtttcg
     6301 ccccaggtca gacggcggtc aatagagacg gcgcagctat ctgctacagc gcaacggctt
     6361 ggggatgtga agaagatttc agaaacggtg agggtgcctt tgccgaggaa ttcgtcataa
     6421 cccagacgtt gggagagttc ttgtaattcg ccaagaatcg gacccatttt gaaaatggcg
     6481 ttgtcaccgc gttctggcgc agaaccgtgg cagctaacac cctgaacatc aatacgaatt
     6541 tccatacgac cgcgctgacc acggtatatc tggcagtcgg ttggttcggt actgaccaca
     6601 aattccgggc gaatgccgga ttgttcaata atgtactgcc agcacagacc gtcgcagtct
     6661 tcttcctgca cagtaccggt aaccagcagg gtatattcat cttccagacc gaggtcttta
     6721 atgattttac cggcataaac catagatgcc atgccgcctt cctggtcgga agtaccgcga
     6781 ccaccgatta gctcatcagt ttccatgcct tcgtacggat cgaagtccca gtttttgatg
     6841 ttgccaatgc cgacggtatc gatatgagcg tccattgcca ccagacgcgg gccgtggccg
     6901 atataaccga gaacgttgcc catcgggtcg atttcaactt tatcgaagcc gactttttcc
     6961 atctcttctt taatacgatg tactacgcgt ttttcgtcgc agctttcact gggaatagca
     7021 accatgtcgc gcaggaagcg agtcatgtcc gcctggtaat cttttgcttt ttcaagaatc
     7081 agtttgaatg gaatattctt agccattatt tttctccaga ttttttactc cccgttttgc
     7141 agggagtagt tccaataggg tgattaaggt gctacagcgt gtttgccttc ccagacaact
     7201 tcgcggtagt gcttcacgtc ggtgtcgcct tcagtactga taaccagcac tacggcatct
     7261 ttgttcagcg ccagtttttc catcaggctt tgacgttgcg ggtgataatg aaccgctgcg
     7321 agaacgccca aaccgacagc gccggattca ccggagatga tgcgcgggtc gttgccgtac
     7381 ggattaccca gcacgcgcat acctaatgcg gcaacgctgt cctggcagga gatgaattgg
     7441 gtggcgcagt tacgtaggat ttcccagccc agcgggttag gttcgccaca ggccaggccc
     7501 gccatgatgg tggccatatc accgccaacg ttgacgatgt cgcctttgac gccggagcga
     7561 taaatacagt cagctttgtc aggttcaaca ataatgctgt gcagattttg cgggctgtag
     7621 acgtcgacca gataacccag cacaccaccg gccattgctc cgacaccggc ttgcagcaga
     7681 acgtgcgtcg gggttacgcc catttcacgc atttgctcga cggcttcatc tgccagggtt
     7741 gcgtagcctt gcatgatcca ggttgggatt ttggtgtaac cttcccatgc cgtgtcctgt
     7801 accacttccc agccgtgctg ctgcgcgtgt tgcatggtca ggcgaacggt atcgtcatag
     7861 ttcatatccg tgacgatgca ctcggcaccg aggttcagaa tggcgtcaac gcgttcctga
     7921 gcagaacctt tcggcatgta aatcaccgca ttctgtccga gttgctgtgc tgcccacgcc
     7981 acaccgcgcc cgtggttgcc gtcggtggtg gtcgcgaaag tcattttttc gccgatggca
     8041 tttttcaggt gctcaaatga cagcgtttcg atatcaagat gatatttttc acacaataat
     8101 tgagcgatgg cgtacgcacc gccaagcatt ttgaaggcgt tcagaccgaa tcgttttgat
     8161 tcgtctttga cgagaatttt cttcacacca aaaaggtttg cgagatcgtc cagcgcacaa
     8221 agcggtgttg ggcgataacc agctattttc tggtggaact ggcgcgccag tttggcctgg
     8281 ctttgcgaaa agagcggtga tgtttcgccg ttgaaaaatt tgttatcggc gatatcaatc
     8341 ttcaatgaga aaacggacat atcctatcct tttggcctct ggaaatagag ggccgaccag
     8401 ctgatcggcc cgaggggtta tttcacgcgt tcttgcgctt ctttcagcag ttgctcgagc
     8461 agtgcaccag gtttggcgta tttacgggac aggatcatcg cggcgatgat ataaggcttc
     8521 cagctggctt ctttgtaggt agcgatacgg tatttttcga atacgccttc agtcacttca
     8581 ccttctttgc aggatacgcc gctgatatca gctggcaggc agtgcatgta cagggcttcg
     8641 ccatcacggg tcagttccat catctcttca gtacaatgcc agtctttgtg ttgcgcgttc
     8701 tgtgccagac actgtttttc cagtgctttt aagccttcgt ggtcgttcgc acgcagcaat
     8761 tcagtacgct cttccatcac tttgtaaggt gcccatgact tcggataaac gatgtctgcg
     8821 tctttgaagg cttcttccat gctggtgacc tgacggaagc taccaccgga ggctttagca
     8881 ttgtttttcg ccacttcaac cacatccggg atcaggtcgt agccttccgg atgggccagg
     8941 gtgacatcca taccgaagcg agtcatcaga ccgatgatgc cttgtggtac agagagcggt
     9001 ttgccatagc ttggagagta ggcccaggtc atggcgattt ttttaccttt caggttttcc
     9061 agtgaaccaa agtgttcacg taaccacgcg aggtcagcca ttgactgagt cgggtggtca
     9121 atatcgcatt gcaggttcac taaagccgga cgctgtggca gtacaccctg cttgtaaccg
     9181 tcatcaagtg cagcgccaac ttcacgcata taggcgttgc ctgcgcccag atacatatcg
     9241 tcgcgaatac caatagcgtc ggcgcagaag gagatcatat tggcggtttc acgcacggtt
     9301 tcgccgtgag cgatttgtga tttgccttcg tcgagatctt gttgtgcaag gccgagcagg
     9361 ttaagcgcgg aagcataaga gaagcgggta cgg
//
LOCUS       DAJTMC010000162         9150 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.162, whole genome shotgun sequence.
ACCESSION   DAJTMC010000162 DAJTMC010000000
VERSION     DAJTMC010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9150
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.162"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            <1..869
                     /gene="maeB"
                     /locus_tag="OO130_003443"
     CDS             <1..869
                     /gene="maeB"
                     /locus_tag="OO130_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HCR5366716.1"
                     /translation="LIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFKEYWTEYF
                     QIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSVVKNVFGY
                     RDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFGIEPRVAL
                     LSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDRMPDSPLK
                     GSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASVRRIVNMV
                     ALAVVEAQTQPL"
     gene            1161..1496
                     /gene="eutS"
                     /locus_tag="OO130_003444"
     CDS             1161..1496
                     /gene="eutS"
                     /locus_tag="OO130_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HCR5366717.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            1509..1988
                     /gene="eutP"
                     /locus_tag="OO130_003445"
     CDS             1509..1988
                     /gene="eutP"
                     /locus_tag="OO130_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HCR5366718.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            1963..2664
                     /gene="eutQ"
                     /locus_tag="OO130_003446"
     CDS             1963..2664
                     /gene="eutQ"
                     /locus_tag="OO130_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HCR5366719.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGAMQPSFKSVTGKGGIKVIDGSSVKFGRFDGAEPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGVLHVRHEGQTMIAKAGDVMFIPKGSSIEFGMTSS
                     VKFLYVAWPANWQSL"
     gene            2661..3470
                     /gene="eutT"
                     /locus_tag="OO130_003447"
     CDS             2661..3470
                     /gene="eutT"
                     /locus_tag="OO130_003447"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001425897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HCR5366720.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQKPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGC
                     QAIVGLSDEDLHRLSHQPLRYLDHDHLVLEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKQPLTVKQIQQRLGERNDY"
     gene            3460..4476
                     /gene="pta"
                     /locus_tag="OO130_003448"
     CDS             3460..4476
                     /gene="pta"
                     /locus_tag="OO130_003448"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HCR5366721.1"
                     /translation="MIIERCRELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSFIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRAITGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YNIAQRLGGYRAVGPLIQGLAVPMHDLSRGCSVQEIIELALVAAVPRQTEVNRESSLQ
                     TLVE"
     gene            4515..4808
                     /gene="eutM"
                     /locus_tag="OO130_003449"
     CDS             4515..4808
                     /gene="eutM"
                     /locus_tag="OO130_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HCR5366722.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            4915..5202
                     /gene="eutN"
                     /locus_tag="OO130_003450"
     CDS             4915..5202
                     /gene="eutN"
                     /locus_tag="OO130_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HCR5366723.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            5214..6617
                     /locus_tag="OO130_003451"
     CDS             5214..6617
                     /locus_tag="OO130_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase EutE"
                     /protein_id="HCR5366724.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPPAAVHEMGVFASLDDAVAAAK
                     VAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQPVLLKNIDERGKGTVSRDWVGRDAAKIA
                     AAIGLNVPQETRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPCIAGLGLGGEGWTTMTITTPTGEGVTSAR
                     TFVRLRRCVLVDAFRIV"
     gene            6628..7464
                     /gene="eutJ"
                     /locus_tag="OO130_003452"
     CDS             6628..7464
                     /gene="eutJ"
                     /locus_tag="OO130_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HCR5366725.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQPVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVAELLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGEEIWPTVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKAEGLY
                     AK"
     gene            7454..9076
                     /gene="eutB"
                     /locus_tag="OO130_003453"
     CDS             7454..9076
                     /gene="eutB"
                     /locus_tag="OO130_003453"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HCR5366726.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGSVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQPRLPLIAYEDDCVT
                     RLIQDDVNETAYNQIKNWSISELREYVLSDETSVDDIAFTRKGLTSEVVAAVAKICSN
                     ADLIYGAKKMPVIKKANTTIGIPGTFSARLQPNDTRDDVQSIAAQIYEGLSFGVGDAV
                     IGVNPVTDDVENLSRVLDTIYGVIDKFNIPTQGCVLAHVTTQIEAIRRGAPGGLIFQS
                     ICGSEKGLKEFGVELAMLDEARAVGAEFNRIAGENCLYFETGQGSALSAGANFGADQV
                     TMEARNYGLARHYDPFIVNTVVGFIGPEYLYNDRQIIRAGLEDHFMGKLSGISMGCDC
                     CYTNHADADQNLNENLMILLATAGCNYIMGMPLGDDIMLNYQTTAFHDTATVRQLLNL
                     RPSPEFERWLESMGIMANGRLTKRAGDPSLFF"
BASE COUNT         2098 a         2384 c         2717 g         1951 t
ORIGIN      
        1 tccttatcgg tcgtccgaac gtgatcgaaa tgcgcattca gaaactgggc ttgcagatca
       61 aagcgggcgt tgattttgag atcgtcaata acgaatccga tccgcgcttt aaagagtact
      121 ggaccgaata cttccagatc atgaagcgtc gcggtgtcac tcaggaacag gcgcagcggg
      181 cgctgatcag taacccgaca gtgatcggcg cgatcatggt tcagcgtggg gaagccgatg
      241 caatgatctg cggtacggtg ggcgattatc atgaacattt tagcgtggtg aaaaatgtct
      301 ttggttatcg cgatggcgtt cacaccgcag gtgcaatgaa cgcgctgctg ctgccgagtg
      361 gtaacacctt tattgccgat acctacgtca atgacgaacc agacgcagaa gagctggcgg
      421 agatcacctt gatggcggca gaaactgtcc gtcgttttgg tattgagccg cgcgttgctc
      481 tgttgtcgca ctccaacttt ggttcttctg actgcccgtc gtcgagcaaa atgcgtcagg
      541 cgctggaact ggtcagggat cgtgcgccag atctgatgat tgatggtgaa atgcacggcg
      601 atgcagcgct ggtggaagcg attcgcaacg accgtatgcc ggacagccct ttgaaaggtt
      661 ctgccaatat tctggtgatg ccgaacatgg aagctgcccg cattagttac aacttactgc
      721 gcgtttccag ctcggaaggt gtgactgtcg gtccggtgct gatgggcgtg gcgaaaccgg
      781 ttcacgtgtt aacgccgatc gcatcggtgc gtcgtatcgt caacatggtg gcgctggccg
      841 tggttgaagc gcaaacccaa ccgctgtaat tcttttttaa ctctcacgct taccctgaat
      901 attcagggta agcggtttgg ctgcaatata ttagtaaagc ttattactga gtttgcgaat
      961 aataaaaaag cagtctatat aatatctcga tattatttta tttatattca tgcgttgcat
     1021 atgaaagttt atgcaccaca gcgaatatct ctccattctt agtgatctac ctcacctttt
     1081 aaacgcgctt gccgaatttt gttatttact ctgacgaaaa attgtcacga tacacgaaag
     1141 tttttcacag gcggcgactc atggataaag aacgcatcat tcaggaattt gtgcccggca
     1201 aacaggtcac actggcgcat ctcatcgcgc atcccggtga ggaactggcg aagaaaatcg
     1261 gcgttccgga tgcgggcgca attggcatta tgacgctaac tcccggcgaa acggcgatga
     1321 tcgccggtga tttagcgctg aaagccgctg atgtgcatat cggttttctc gaccgcttca
     1381 gcggtgcatt ggtgatttat ggctcggtgg gcgcagtaga agaagcgtta tcacaaaccg
     1441 tcagcggttt aggtcgctta ttaaattata cgctttgtga aatgaccaaa agttaatacg
     1501 aggcattcat gaaacgaatt gcgtttgtgg gtagtgtcgg ggcaggaaaa acaacgttat
     1561 ttaatgcgtt gcaggggaat tataccctcg ccagaaaaac acaggccgtg gaatttaatg
     1621 ataagggcga tattgatacg ccgggtgaat attttagcca tccccgctgg tatcacgcct
     1681 taattaccac gctacaggat gtagacatgc tgatctatgt ccatggcgca aatgacccgg
     1741 aaagtcggtt gcctgccggg ttgctggata ttggcgtcag caaacggcaa atcgccgtca
     1801 tcagtaaaac ggacatgcca gatgctgacg tcgccgcaac gcgaaagtta ttgcttgaga
     1861 cgggtttcga agagccgatt ttcgagctaa acagccacga cccgcaaagc gtgcagcaac
     1921 tggtggatta tctggcatct ctcaccaaac aggaggaagc aggtgaaaaa actcatcaca
     1981 gcgaatgata ttcgtgaagc acacgcgcgc ggcgaacagg caatgtccgt ggttctgcgc
     2041 gccagcatta ttaccccgga agcccgtgaa gtggcggatc tactgggttt taccatcacc
     2101 gaatgtgatg aatcgatccc ggtaacggcg tccgtgcccg ccagcgtgcc agccgacaaa
     2161 accgaaagcc agcgcatccg cgaaaccatc attgcccagc tgccggaagg ccagtttacc
     2221 gaaagcctgg tcgcacagct gatggaaaaa gtgatgaagg aaaaacagtc gctggagcag
     2281 ggcgcaatgc agccgagctt caaatcggtg accggcaaag gcggcatcaa agtgattgat
     2341 ggcagcagcg tcaagtttgg tcgtttcgac ggcgcagagc cgcattgcgt gggcttgact
     2401 gatcttgtga ccggagacga cggcagcagc atggccgccg gttttatgca gtgggagaac
     2461 gcattcttcc cgtggaccct gaactacgac gaaatcgaca tggtgctgga aggggtgctg
     2521 catgttcgcc acgaaggcca aaccatgatc gccaaagcag gcgacgtgat gtttatcccg
     2581 aaaggctcca gcatcgaatt tggtatgaca tccagcgtga aattcctgta tgtcgcctgg
     2641 ccggctaact ggcaatccct atgaaagatt tcatcaccga agcatggcta agagcgaacc
     2701 atacgctcag cgaaggcgca gagatccatc tccctgcgga cagtcgcctg acgccctctg
     2761 cccgggagtt gctggaaagc cgccatctgc gcatcaagtt tattgacgag cagggccgcc
     2821 tgtttgttga cgatgaacag cagaagccgc agcccgttca tgggctgacc agtagcgatg
     2881 aacatccgca ggcgtgctgc gaactgtgcc gccagccggt ggcaaaaaag ccggatacgc
     2941 tgactcacct gtcggcggaa aaaatggtcg ccaaaagcga tccgcgtctg ggttttcgcg
     3001 cggttctcga cagcaccatt gcgctggcag tgtggctgca aattgaactg gcggaaccgt
     3061 ggcagccgtg gctggcggat atccgttcgc gtcttggcaa cattatgcgc gccgatgcgc
     3121 tgggggaacc gctgggctgc caggcgattg tcgggctttc tgacgaagat ctgcatcggc
     3181 tttctcatca gccgctgcgc tatctcgacc acgatcatct ggtgctggaa gccagccacg
     3241 ggcgcgatgc tgcgttgctc aatctgctac gcaccaaagt gcgggaaacc gaaaccgtag
     3301 cggcgcaggt ctttatcacc cgcagctttg aagtattacg cccggacatt ttgcaggcgc
     3361 tgaaccgcct ttccagcacg gtctacgtga tgatgatcct gagcgtaacg aagcagccgc
     3421 taacggtgaa gcagatccaa caacgactgg gggaacgcaa tgattattga acgttgtcgt
     3481 gaactggcgt tgcgagcgcc cgccagagtg gtttttccgg atgcgttaga tcaacgtgtg
     3541 ctgaaagccg cgcaatattt acatcaacaa ggtctggcaa cgcccattct ggtcgccaat
     3601 ccgtttgaac ttcgtcagtt tgcgctcagt cacggcgtgg cgatggacgg gctacaggtg
     3661 attgatccgc atggcaacct cgcaatgcgg gaagaatttg ctcatcgctg gctggcccgc
     3721 gcgggcgaaa aaacgccgcc ggatgcgctg gaaaaactta ccgaccctct gatgttcgcc
     3781 gcagcaatgg tcagcgccgg taaagcggat gtctgtatcg caggcaacct ctcttccacg
     3841 gcaaatgtgc tgcgtgccgg attacgcatt atcggcttgc agccaggctg taaaacgctc
     3901 tctttcattt tcctgatgtt gccacagtac agcggcccgg cgttgggctt tgccgattgc
     3961 agcgtggtgc cacagccgac ggcggcgcag ctggcggata tcgcgcttgc cagtgccgaa
     4021 acctggcgcg ccatcaccgg agaagagccg cgcgtggcga tgctgtcgtt ttccagcaat
     4081 ggtagcgccc gtcacccctg tgttgctaac gtccagcagg cgacagaaat cgtccgtgag
     4141 cgcgcaccaa agctggtggt ggatggcgag ttacagtttg acgccgcctt cgtgccggaa
     4201 gtggcggcgc aaaaagcgcc tgccagcccg ctacagggca aggccaatgt gatggttttt
     4261 ccgtcgctgg aagccggaaa tattggttac aacatcgcac aacgactcgg cggatatcgt
     4321 gccgtcgggc cactgataca aggacttgcc gtgccgatgc acgatctctc tcgtggttgt
     4381 agcgtgcagg aaattatcga gctggcgctg gtggcagctg tgccgcgtca gacagaagtg
     4441 aaccgcgaaa gcagtttaca aacactggtt gaatgaacgg tcccgttctg gaccctgata
     4501 aagaggaaag cacaatggaa gcattaggaa tgatcgaaac ccggggcctg gttgcactga
     4561 ttgaggcttc tgacgcgatg gtgaaagctg cgcgcgtcaa gctggttggt gttaagcaga
     4621 ttggcggcgg cctgtgtact gccatggttc gtggtgatgt tgcagcctgt aaagccgcga
     4681 ccgatgcagg tgccgcagcg gcacagcgta tcggcgagct ggtatctgta cacgttatcc
     4741 cgcgtccgca cggcgacctg gaagaagtct tcccgatcgg cctgaaaggc gatagcagca
     4801 acctgtaatc accctgtgtg acgtcgattc gggcgttacg ctctctcctt tttcatttta
     4861 ccttctgcgg agggtagggg gagcttttac ctgaaatacg ccacggaggc gggtatgaaa
     4921 ctggcagtcg tcactggaca aattgtttgt accgtacgcc atcacggact ggcgcatgac
     4981 aaattgctga tggtggaaat gattgatcca caaggtaatc ctgacgggca atgcgccgtc
     5041 gccatcgaca atattggcgc gggaaccggg gagtgggtgt tactggtgag tggcagttcc
     5101 gcccgccagg cgcataaaag cgaaacgtca ccggtcgatc tgtgcgtgat tggcattgtc
     5161 gatgaggtgg tgtctggcgg tcaggtaatt ttccacaaat aaggcagaac atcatgaatc
     5221 aacaggatat tgaacaggtg gtgaaagcgg tactgctgaa aatgcaaagc agtgacacgc
     5281 cgcccgccgc cgttcatgag atgggcgttt tcgcgtccct ggatgacgcc gttgcggcag
     5341 ccaaagtcgc ccagcaaggg ttaaaaagcg tggcaatgcg ccagttagcc attgctgcca
     5401 ttcgtgaggc aggcgaaaaa cacgccagag atttagcgga acttgccgtc agtgaaaccg
     5461 gcatgggccg cgttgaagat aaatttgcca aaaacgtcgc tcaggcgcgc ggcacgccag
     5521 gcgttgagtg tctctctccg caggtgctga ccggcgacaa cggcctgact ttaattgaaa
     5581 acgcgccctg gggcgtggtg gcttcggtaa ctccgtccac taacccggcg gcaaccgtaa
     5641 ttaataacgc catcagcctg attgccgcgg gcaacagcgt catttttgcc ccgcatccgg
     5701 cggcgaaaaa agtctcccag cgggcgatca cgctgctcaa ccaggcgatt gttgccgcag
     5761 gcgggccgga aaacttactg gttactgtgg caaatccgga tatcgaaacc gcgcagcgtt
     5821 tgttcaagtt tccgggcatc ggccttctgg tggtgaccgg cggcgaagcg gtagtagaag
     5881 cggcgcgtaa acacaccaat aaacgtctga ttgccgcagg cgctggcaac ccgccggtag
     5941 tggtggatga aaccgccgac ctcgcccgtg ccgctcagtc catcgtcaaa ggcgcttctt
     6001 tcgataacaa catcatttgt gccgacgaaa aggtactgat tgttgttgat agcgtagccg
     6061 atgaactgat gcgtctgatg gaaggccagc acgcggtgaa actgaccgca gaacaggcgc
     6121 agcagctgca accggtgttg ctgaaaaata tcgacgagcg cggaaaaggc accgtcagcc
     6181 gtgactgggt tggtcgcgat gcggcgaaaa tcgcggcggc aatcggcctg aacgtgccgc
     6241 aagaaacgcg tttgctgttt gtggaaacca ctgcagaaca tccgtttgcc gtgaccgaac
     6301 tgatgatgcc ggtgctgccc gtcgtgcgcg tcgccaacgt ggcggatgcc attgcgctgg
     6361 cggtgaaact ggaaggcggt tgccaccaca cggcggcaat gcactcgcgc aacatcgaaa
     6421 acatgaacca gatggcgaat gctattgata ccagcatttt cgttaagaac ggaccgtgca
     6481 ttgccgggct ggggctgggc ggggaaggct ggaccaccat gaccatcacc acgccaaccg
     6541 gtgaaggggt aaccagcgcg cgtacgtttg tccgtctgcg tcgctgtgta ttagtcgatg
     6601 cgtttcgcat tgtttaagga gtaacagatg gcgcacgacg aacaatggct caccccacgt
     6661 ctgcaaacgg cggcaacgct gtgtaaccag acgcccgccg cgacggaatc tccgctgtgg
     6721 ctgggggtgg atctgggcac ctgcgatgtg gtgtcgatgg ttgtcgaccg cgacggtcag
     6781 ccggtggcgg tatgcctcga ctgggccgat gtggtccgcg acggcatcgt ctgggatttc
     6841 ttcggcgctg tcaccattgt tcgtcgccat ctcgacacgc tcgagcagca attcggtcgc
     6901 cgttttagcc atgcggcgac ttcatttcct ccaggtacgg acccgcgtat ttccatcaac
     6961 gtgctggagt ctgctggact ggaagtgagc catgtgctgg atgagccaac cgcagttgcg
     7021 gagctgctgc aactggacaa cgccggtgta gtggatatcg gcggcggcac taccggcatt
     7081 gccatcgtga aaaagggcaa ggtgacgtac tcggcggatg aagcaaccgg cggacatcac
     7141 atctctctga ctctcgccgg aaatcgccgt atttcgctgg aagaggcgga gcagtacaag
     7201 cgcggtcacg gtgaagagat ttggcctacg gtgaaaccgg tgtacgaaaa aatggcggac
     7261 attgtcgccc ggcatattga agggcagggc attactgatt tatggctggc gggcggctct
     7321 tgtatgcaac cgggcgtggc ggaactgttt cgcaaacaat tcccggcgtt acaggtgcat
     7381 ttaccgcagc acagtttgtt tatgaccccg ctggcgatcg ccagtagtgg gcgagagaaa
     7441 gcggaggggc tatatgcaaa atgaattgca gaccgcgctc tttcaggcgt tcgataccct
     7501 gaatctgcaa cgggtaaaaa catttagcgt tccaccggtg acgctttgcg gtccgggctc
     7561 ggtgagcagt tgcggacagc aagcgcaaac gcgtgggctg aaacatctgt tcgtgatggc
     7621 agacagcttt ttgcatcagg cagggatgac cgccgggctg acgcgcagcc tggctgttaa
     7681 aggcatcgcc atgacgctct ggccatgtcc ggtgggcgaa ccgtgcatca ccgacgtgtg
     7741 tgcagccgtg gcgcagttgc gtgagtcagg ctgtgatggg gtgatcgcat ttggcggcgg
     7801 ctcggtgctg gatgcggcga aagccgtggc gttgctggtg acgaacccgg atagcacgct
     7861 ggcagagatg tcagaaacca gcgttctgca accgcgcttg ccgctgattg cctatgaaga
     7921 tgactgcgtg acgcggctga ttcaggacga tgttaacgaa acggcctaca accagattaa
     7981 aaactggagc atcagcgaac tgcgtgagta tgtgctgagc gatgaaacta gcgtggacga
     8041 cattgccttt acccgcaaag ggctgacctc ggaagtggtc gcggcggtag cgaagatttg
     8101 ctccaacgcg gacctgatct acggcgcgaa gaaaatgccg gtaatcaaaa aggccaatac
     8161 caccatcggt attccgggca ccttcagcgc ccgtttgcag ccaaatgaca ctcgtgacga
     8221 cgtgcaaagt atcgccgcgc aaatctacga agggctttcc ttcggggtgg gcgatgcggt
     8281 gatcggcgtc aacccggtaa ctgacgacgt ggaaaactta agccgcgtgc tggataccat
     8341 ttatggcgtg atcgacaaat tcaacatccc aactcagggc tgcgtactgg cgcacgtcac
     8401 cacccagatc gaagcgattc gtcgcggcgc gcctggcgga ctgattttcc agagtatctg
     8461 tggcagcgaa aaagggctga aagagtttgg cgtggaactg gcgatgctcg acgaagcgcg
     8521 cgcagtgggt gcagagttca accgtatcgc cggggaaaac tgcctctact tcgaaaccgg
     8581 acaaggctct gcgctatccg ctggcgctaa cttcggcgct gaccaggtga cgatggaagc
     8641 acgtaactat gggctggcgc gtcattacga tccgtttatc gtcaacaccg tggttggctt
     8701 tattgggccg gagtatctct acaacgaccg ccagattatc cgtgctggct tagaagatca
     8761 ctttatgggc aagctgagcg gcatctctat gggctgtgac tgctgctaca ccaaccacgc
     8821 tgacgctgac cagaacctca acgaaaacct gatgatcctg ctcgccaccg caggctgcaa
     8881 ctacatcatg gggatgccgc tgggtgatga catcatgctc aactaccaga ccaccgcatt
     8941 ccacgatacc gccactgtgc gtcagttact caacctgcgc ccgtcaccag agtttgaacg
     9001 ctggctggaa agcatgggca ttatggcaaa cggtcgcctg accaaacggg cgggcgatcc
     9061 gtcactgttc ttctgatgac gcggagataa cacatcatgg atcaaaaaca gattgaagaa
     9121 attgtacgca gcgtgatggc gtcaatggga
//
LOCUS       DAJTMC010000163         9089 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.163, whole genome shotgun sequence.
ACCESSION   DAJTMC010000163 DAJTMC010000000
VERSION     DAJTMC010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9089
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.163"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            52..936
                     /locus_tag="OO130_003454"
     CDS             52..936
                     /locus_tag="OO130_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HCR5366727.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHHEDADGVALTFELTQSEETKKFCPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSISMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(987..1841)
                     /gene="yeaE"
                     /locus_tag="OO130_003455"
     CDS             complement(987..1841)
                     /gene="yeaE"
                     /locus_tag="OO130_003455"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HCR5366728.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLRDNVFLVSKVYPWNAGRQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLDYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISSAQVLLARVISHQGVMAIPKAATIAHVQQNAAVLEVELSSAELTMLDKAYPAPK
                     GKTALDMV"
     gene            complement(1931..2677)
                     /gene="mipA"
                     /locus_tag="OO130_003456"
     CDS             complement(1931..2677)
                     /gene="mipA"
                     /locus_tag="OO130_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HCR5366729.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            3113..5047
                     /gene="yeaG"
                     /locus_tag="OO130_003457"
     CDS             3113..5047
                     /gene="yeaG"
                     /locus_tag="OO130_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HCR5366730.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            5160..6443
                     /locus_tag="OO130_003458"
     CDS             5160..6443
                     /locus_tag="OO130_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HCR5366731.1"
                     /translation="MTWFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAMNKRSVT
                     DVDSGESISIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
     gene            6590..8065
                     /locus_tag="OO130_003459"
     CDS             6590..8065
                     /locus_tag="OO130_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HCR5366732.1"
                     /translation="MIQSTRISMGLFFKYFLSLTKIDPSQNYISLPSIKSSTHIALLF
                     MVSMGTQKLKAQSFFIFSLLLTLILFCITTLYNENTNVKLIPQMNYLMVVVALFFLNA
                     VIFLFMLMKYFTNKQILPTLILSLAFLSGLIYLVETIVIIHKPINGSTLIQTKSNDVS
                     IFYIFRQLSFICLTSLALFCYGKDNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYN
                     ADYSLYVVDYCPDNHTATRGINYTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLA
                     SLCCNLLLLTLDEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQELQLSSKLAVHDVLT
                     NIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQFKRINAQWGHRVSDKVLVSIVDIIQ
                     QSIRPDDILARLEGEVFGLLFTELNSAQAKIIADRIRKNVELLTGFSNRYDVPEQMTI
                     SIGTVFSTGDTRNISLVMTEADKALREAKSEGGNKVIIHHI"
     gene            8159..8245
                     /locus_tag="OO130_003460"
                     /pseudo
     CDS             8159..8245
                     /locus_tag="OO130_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459804.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            8246..>9089
                     /locus_tag="OO130_003461"
     CDS             8246..>9089
                     /locus_tag="OO130_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCR5366733.1"
                     /translation="MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQ
                     KADSSFLYDKYQNQSIAAHVMRALAAEQSEVSPEQRRAICEAFESANNTHGLNLTAHK
                     YPGLRGTLQTASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDL
                     NDRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQN
                     VMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGREVAK
                     FSVPL"
BASE COUNT         2394 a         2181 c         2106 g         2408 t
ORIGIN      
        1 cttcggtcgc tctttttttt acctgataaa atgaagttaa aggactgcgt catgattaag
       61 aaaatttttg cccttccggt catcgaacaa atctcccctg tcctctcccg tcgtaaactg
      121 gatgaactgg acctcattgt ggtcgatcat ccccaggtaa aagcctcttt tgcattacag
      181 ggcgcacacc ttctctcgtg gaaacctgcg ggtgaagaag aagttctgtg gttgagcaac
      241 aacacaccgt tcaaaaatgg cgtcgctatt cgcggtggcg taccggtttg ctggccgtgg
      301 tttggtccgg cggcacaaca aggtctgcct gcgcacggtt ttgcccgcaa cctgccgtgg
      361 acgctgaaat cacatcatga agatgctgat ggcgtagcgc tgacttttga attgacgcaa
      421 agcgaagaga cgaaaaaatt ctgtccgcac gactttacgc tgttagcgca tttccgcgtg
      481 ggtaaaacct gtgaaatcga tcttgaatca catggcgaat ttgaaaccac ctctgccctg
      541 catacctact ttaacgtggg tgatatcgct aaggtaagcg tcagtgggct gggcgatcgc
      601 ttcattgata aagtgaatga cgcgaaagaa gatgtactga ccgacggtat tcagaccttc
      661 cctgaccgta ccgatcgcgt gtatctgaat ccgcaagatt gcagcgtgat taatgatgaa
      721 gcgctgaatc gtattatcgc cgtaggccac cagcatcatc tgaacgttgt aggctggaac
      781 ccgggaccgg cgctttcaat tagcatgggc gatatgccgg atgatggcta caaaacattt
      841 gtttgtgtag aaacggctta cgcttcagaa acgcaaaaag tcaccaaaga gaaacctgca
      901 catctggcgc aatccattcg cgttgcgaaa cgttaatttg cgttaatgtt gtgtgccggg
      961 tgcgatgcat ccggcacgta tcaacatcac accatatcca gcgcagtttt tccttttggt
     1021 gccggatacg ctttatccag catggttaat tccgctgacg aaagttcgac ctcaagcaca
     1081 gccgcattct gttgaacatg ggcaatcgtg gccgcttttg gaatcgccat cacaccctga
     1141 tggctgatta ccctcgccaa caatacttgt gccgagctga tattgtgagc atgtgcaatt
     1201 tcgttgacta ccgcgttttt taacagtcca ttgcgcaacc gcccggcctg ggctaatgga
     1261 ctgtaagcca tcaccggcat ctgctgttgc tggcaccagg ggagtagatc gtactcaatc
     1321 cctcgtgaac cgagatggta aagcacctga ttagtggcac actgatttcc ccccggcagc
     1381 tgccagagtt cctgcatatc agcataatca aggttagaaa cgccccagcg gcggattttt
     1441 ccctgggcga tcaatttttc catcgctgca acagtctctt caaaagcgaa actgccagac
     1501 cagtgtaata agtagagatc gagataatca gtattgagac ggcgtaaact ggcttcgcat
     1561 gcatttatcg ctttttgcct gccagcattc cacggataga ctttagagac gagaaagacg
     1621 ttatcacgca gaccggttaa tgcttcccca accacctttt cggcaccgcc atcggcatac
     1681 atttcggcgg tatcaatgag ggttaaaccg agctcaatgc ccgcgcgtag tgcagcaact
     1741 tctgttttgc gctgactggc atcttcgccc atataccatg ttccctgccc tatggctggc
     1801 agtgagacat cgccactaaa ttgaatcatt ttttgttgca ttgtttcctc ccaggtattg
     1861 caccaccgta atgcaaaaca gggcgtgacg ccctgttttt atgcacaaaa tgccctggaa
     1921 agatgcatta tcagaatttg taggtgatcc cggtagaaat caggccagtc caggatttat
     1981 ccaccatcgg gctgtcagta acttcatcag acagacgggt atagcgcgcg gtaccgtaaa
     2041 cactccagtc gccgaggaag ttgtagctgg cgctcagctc caggtaaggg ctccagctgt
     2101 cgttcgggtt atagccacgc agaccgctgc gagcggactc tttgcgcgat acgccataat
     2161 agtattcgtt ctggttttcg ctgttccact gcacaccaat acccggagtc acggtcaggc
     2221 caccgttggt gtaacgatac aaccaggcca tatcccagac aatgccgttg ctgttatcca
     2281 gggtatcgcc agccagggtg gtacgcaggt aaccgtactg ggtaaagtga gcataagaca
     2341 gaccagccat catggtgctc ttacggtcat ccaggtgacg catttggtga tcgccactgt
     2401 ctttcgcttt gaagtaaagc ggcgaccagt aagcggtaat tgaaagttta tccgttgcgt
     2461 cattccacag gtagtaacca ccacctaagc cacggaacca gaagttatcg ccttcatagt
     2521 tgattaccgg tactgggtaa acatcggtat cgtaatcttt atatgggtgc tcaacgacac
     2581 ctacgcctgc gcccaaggaa aatttacctt cagcgtgcgc tacgcctgca gacgttgcga
     2641 taagcactcc aagtgccaga agtttgagtt tggtcacaat taatcattcc ttaaacaaat
     2701 gtttagcggg cgacaaagtt tacccgtcaa tacatcgaaa cccaaccttt ttacgttttc
     2761 attttttaaa gtaactgttt aattttcctg ccgcggatga caccgcgctt acagccaaat
     2821 gaatttagcg ttactggcga gcctggtctt tacattaatt atgcaaaatt tatggatgag
     2881 ttgttgatat gccattgaaa ttaagaaagc cgtgcaggca agttttccat ttgccatcta
     2941 cgcttaattt tgaaggtgta tcaccgggca cgttgttctc atcgtcgata aaatggcatg
     3001 agagttgctg tgttttagca agagacgtcg ttcagtttac ctcttccggg aggctctact
     3061 attcatatga acggctctta acctgtgcta aaaaacgaaa ggacggcata ccatgaatat
     3121 attcgatcac tatcgccagc gatatgaagc tgccaaggac gaagagttca cactgcagga
     3181 gtttcttacc acttgtcggc aagatcgcag tgcttatgcc aacgcggctg agcggctatt
     3241 gatggctatc ggtgagcctg tcatggtcga tacagcccag gaacccagac tttctcgact
     3301 cttttctaac cgggtcattg cacgttatcc ggcgtttgaa gagttttacg gcatggaaga
     3361 cgcgattgaa cagattgtct cttatctgaa acacgcggct caggggctgg aagagaagaa
     3421 acaaatcctg tatctgctgg ggcctgtggg tgggggtaaa tcatcgcttg ctgagcgact
     3481 gaaatcatta atgcagctcg taccgattta tgtattgagc gccaacggtg agcgtagccc
     3541 ggtcaacgat catccgttct gtcttttcaa tccgcaggaa gatgcgcaga ttctggaaaa
     3601 agagtatggc attcctcgcc gttatctcgg caccatcatg tcgccgtggg cggcaaaacg
     3661 cctgcatgaa tttggtggcg atatcactaa gttccgggta gtgaaggtct ggccgtcaat
     3721 tctgcaacaa attgctatcg ccaaaacgga acccggcgat gagaacaacc aggacatctc
     3781 cgcgctggtt gggaaagtcg atattcgtaa actcgaacac tacgcgcaga atgacccgga
     3841 cgcctacggc tattccggtg cgctgtgccg cgccaatcag gggatcatgg aattcgttga
     3901 gatgtttaaa gcaccgataa aagtgctgca tcccttgtta accgccactc aggaaggtaa
     3961 ctacaacggg acggaaggta tctccgccct gccgttcaac gggattattc tcgcccactc
     4021 gaacgagtcc gaatgggtca ctttccgtaa taacaaaaac aacgaagcct tcctcgaccg
     4081 tgtttacatc gtgaaggtgc cgtattgctt gcgcatttcc gaagagatca aaatctacga
     4141 gaaattgctt aatcacagtg aattgactca cgccccatgc gcccctggca cgctggaaac
     4201 actgtcacgt ttttccattc tttcgcgcct gaaagagcca gaaaactcca gcatttattc
     4261 aaagatgcgg gtttatgatg gcgaaagtct gaaagacacc gatcccaaag ccaagtcgta
     4321 tcaggaatat cgtgactacg ccggtgtcga tgaagggatg aacggtctgt cgacgcgttt
     4381 tgcgtttaag atcctctccc gcgtgttcaa cttcgatcat gtagaagtgg cggcaaaccc
     4441 ggtccatctg ttctacgtcc tggaacagca gatcgagcgc gagcagttcc cgcaagagca
     4501 ggcagaacgc tatctggagt tcctgaaagg ttatctgatc ccgaaatatg ccgagtttat
     4561 cggcaaagag atccagacgg cctaccttga atcctattcc gaatatgggc aaaacatttt
     4621 cgaccgttat gttacctacg cggatttctg gattcaggat caggagtatc gcgatccgga
     4681 taccgggcag ctgtttgacc gcgagtctct taacgccgag ctggagaaaa tcgagaaacc
     4741 ggcggggatc agtaatccaa aagatttccg caacgagatt gttaacttcg tactgcgcgc
     4801 cagagcgaat aacagcggac gcaatccgaa ctggaccagc tatgaaaaac tgcgcacggt
     4861 tatcgagaag aaaatgttct ccaataccga ggagctgttg ccggttatct cgtttaacgc
     4921 caaaacgtca accgacgagc agaagaaaca cgacgacttt gtcgaccgta tgatggaaaa
     4981 aggctacacc cgtaaacagg tgcgtttact gtgcgaatgg tatttgcgcg tacgtaaatc
     5041 gtcttaacaa ccctggcccg gtacacatgc taccgggcct acaacgacag cgaaccgtgg
     5101 gccagagaaa tggcaacaca ggcgtagcat acagttggca aatgtagtac ggggggcata
     5161 tgacctggtt tattgaccgg cgtctgaacg gcaaaaacaa aagcatggtg aatcgccagc
     5221 gttttttacg ccgttataaa gcgcaaatta aacagtcgat ctccgaggcc atgaataagc
     5281 gttcggtgac tgacgtcgac agcggcgaat ccatatccat tcccacggaa gatattagcg
     5341 aaccgatgtt tcatcagggg cgtggcggcc tgcgccaccg cgtacatccg ggcaatgacc
     5401 atttcgtcca gaacgaccga attgaacgtc cccagggtgg cggcggaggt tccggcagtg
     5461 gtcagggcca ggccagccag gatggtgaag gtcaggatga atttgtcttt cagatttcga
     5521 aagatgagta tcttgatctg ctctttgaag atttggcctt accgaatctg aaacaaaacc
     5581 aacaacgcca gctgaccgaa tataaaacgc atcgggcggg ttataccgct aacggcgttc
     5641 cagccaatat cagcgttgtg cgttccttgc agaactcact ggcgcgacgc acagccatga
     5701 cggcgggcaa gcgacgggaa cttcatgcgc tggaagagaa tttggccatc atcagcaaca
     5761 gtgaacctgc gcaactgctg gaagaggaac gtctgcgcaa agaaattgca gaattacgtg
     5821 ccaaaattga acgcgtccct tttattgaca ccttcgattt acgttacaag aactacgaga
     5881 agcggcccga tccctccagc caagcagtga tgttttgcct gatggacgtt tccggttcaa
     5941 tggatcaatc cactaaagat atggctaagc gtttttatat tctgctgtat ctgttcctca
     6001 gcagaacgta taagaacgtg gaagtcgtat acatccgcca tcatacccaa gcgaaagaag
     6061 tcgatgaaca tgagtttttc tactcgcagg aaacaggcgg caccattgtt tccagcgccc
     6121 tgaaactgat ggatgaggta gtgaaagagc gttataaccc ggcacagtgg aatatttacg
     6181 ctgcacaagc atcggacggc gataactggg ccgatgactc tccgctttgc catgaaatcc
     6241 tggcgaaaaa attattacct gttgttcgtt attacagcta tatcgaaatt acccgtcgtg
     6301 cacatcagac attgtggcga gaatatgagc atctgcaatc tactttcgac aactttgcga
     6361 tgcagcacat ccgcgaccag gatgatattt atccggtgtt ccgtgaactg tttcataaac
     6421 aaaatgcaac agctaaagac taaaactatc agccaggtca ttatcgcctg gctgattttt
     6481 agcttactgt aaattatctc atttattaca tacattagct tacaatcgct ttaaatatga
     6541 cagcataacc tttacataat ttagttccag aaaacaatca ttcggaaaaa tgattcagtc
     6601 aacacgtatt tccatggggt tattctttaa atatttttta tcgttaacga aaattgatcc
     6661 tagtcaaaac tatatatctc tgccatcaat aaaatccagc actcacattg ctctcctttt
     6721 tatggtttct atgggtacac aaaaattaaa agctcaaagc ttttttattt tcagtttatt
     6781 gctgacatta attttatttt gcattactac cttatataac gaaaacacaa atgtaaaact
     6841 catcccacag atgaattacc tgatggttgt tgtggctttg tttttcctta acgccgtcat
     6901 ttttcttttc atgttaatga aatatttcac taacaaacaa attttaccaa cactcatttt
     6961 aagccttgca ttcttaagtg gccttatcta tttagttgaa accattgtaa ttatccataa
     7021 accaattaac ggcagtacac tgatccagac aaagtcgaat gatgtttcta ttttctatat
     7081 tttccgccaa ctcagtttta tttgtttaac ctcgctggca ctcttttgtt atggaaaaga
     7141 caacatcctt gacaacaata agaaaaaaac gggaatcctg ttgctggcgt tgattccttt
     7201 tttagttttt ccccttctgg cacacaatct gagcagttat aacgctgact attctttgta
     7261 tgtcgtcgac tactgtccgg acaaccatac tgcgaccagg ggaatcaact atacaaaaat
     7321 attggtttgt ctgtgggcat ttttactgtt ctttattatc atgcgtacac gattagccag
     7381 cgaactatgg ccgttaatag cattattatg tctggcatcg ctatgctgca acttacttct
     7441 actgactctg gatgagtata attacaccat ctggtatatc agtcgcggga ttgaagtttc
     7501 cagtaaactg tttgttgtgt cttttctgat ttataacatt tttcaggagc tgcaactctc
     7561 cagcaaactg gcagttcatg atgtgctgac caatatttat aatcggcgct actttttcaa
     7621 cagcgtagag tcattattgt cgcgacctgt tgttaaggac ttctgtgtca tgctggttga
     7681 tattaatcag ttcaaacgca tcaatgccca atggggacat cgtgtgagtg ataaagtgct
     7741 ggtttcaatt gtcgatatta tccagcaaag catccgccct gatgatattt tagcgcgact
     7801 ggagggtgag gtgtttggct tgctatttac cgaactcaat agtgcccagg caaaaatcat
     7861 tgcggatcgt atacgtaaaa atgtcgaact cctgaccggc tttagtaaca gatatgatgt
     7921 tcctgaacaa atgaccatca gtattggcac ggttttttca acgggtgaca cgcgtaatat
     7981 ctcgcttgtc atgacggaag cagataaagc cttacgcgaa gcgaaaagcg aggggggcaa
     8041 caaagtgatt attcatcata tttaagtgca aaaatattca gagccatgct ttttgcgtgg
     8101 cttttgcata caatttatta ctattacccc taaatttccc ctccccagcg gcgcagagat
     8161 gagtataatt agcgcccctg tgccaggccg caatcgaact ttatctggtt ttctcgtttc
     8221 actaactgaa ggagtgccat ttatcatgaa attgcaccat agaatgctcc ggcattttat
     8281 cgccgcaagt gtcattgtgc tgacatcttc cttccttatt tttgaacttg tcgccagcga
     8341 cagagcaatg agtgcctatc tgcgctatat cgtgcagaaa gcagactcct cctttcttta
     8401 tgataagtat cagaatcaga gtattgccgc gcatgtgatg cgcgctctcg ctgctgagca
     8461 gtcggaagtg tcgccagaac agcggcgcgc catctgcgag gcttttgagt ctgccaataa
     8521 tacccatggc ttaaacctga ctgctcataa atacccgggc ttacgcggca cactacaaac
     8581 cgcatccact gactgcgaca caattgtgga agctgcagca ctattacccg cttttgatca
     8641 ggcagtggaa ggcaaccgcc accaggatga ttacggttca ggtcttggga tggccgaaga
     8701 gaaatttcac tattatctcg atctcaatga ccgctatgtc tatttttatg agccggttaa
     8761 tgttgaatac tttgcgatga ataactggtc cttcctgcag tcaggaagta ttggtatcga
     8821 tcgcaaagat attgaaaagg tatttaccgg gcgtaccgta ttgtcgagca tttaccagga
     8881 tcagcgtact aaacagaacg tgatgagttt gctgacgccg gtgtatgttg cagggcagct
     8941 aaaagggatt gtgctgctgg atattaacaa aaacaatttg cggaatatct tttacactca
     9001 tgaccgccct ctcctctggc gttttctcaa tgtcacgcta accgataccg attcggggcg
     9061 tgaagtggcc aaattcagtg tgccacttc
//
LOCUS       DAJTMC010000164         9046 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.164, whole genome shotgun sequence.
ACCESSION   DAJTMC010000164 DAJTMC010000000
VERSION     DAJTMC010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9046
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.164"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            195..1028
                     /gene="ppsR"
                     /locus_tag="OO130_003462"
     CDS             195..1028
                     /gene="ppsR"
                     /locus_tag="OO130_003462"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HCR5366734.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRTANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            1184..2230
                     /gene="aroH"
                     /locus_tag="OO130_003463"
     CDS             1184..2230
                     /gene="aroH"
                     /locus_tag="OO130_003463"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HCR5366735.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARTSHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            2362..2553
                     /gene="hemP"
                     /locus_tag="OO130_003464"
     CDS             2362..2553
                     /gene="hemP"
                     /locus_tag="OO130_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HCR5366736.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(2557..3993)
                     /locus_tag="OO130_003465"
     CDS             complement(2557..3993)
                     /locus_tag="OO130_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HCR5366737.1"
                     /translation="MTLSFVTRWRDELPETYTALSPTPLNNARLIWHNIELANTLSIP
                     SSLFKNGAGVWGGEALLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TAEPGAMLMRVAPSHLRFGHFEHFYYRRESEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FSDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLIQSVNPALVLRNWLAQRAIEAAEKGDMMELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(4056..4769)
                     /gene="ydiV"
                     /locus_tag="OO130_003466"
                     /pseudo
     CDS             complement(4056..4769)
                     /gene="ydiV"
                     /locus_tag="OO130_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416222.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
     gene            complement(5015..5479)
                     /locus_tag="OO130_003467"
     CDS             complement(5015..5479)
                     /locus_tag="OO130_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HCR5366738.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKQQASIGTQIDKDKLLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            complement(5557..6306)
                     /gene="btuD"
                     /locus_tag="OO130_003468"
     CDS             complement(5557..6306)
                     /gene="btuD"
                     /locus_tag="OO130_003468"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HCR5366739.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLILTI"
     gene            complement(6306..6857)
                     /gene="btuE"
                     /locus_tag="OO130_003469"
     CDS             complement(6306..6857)
                     /gene="btuE"
                     /locus_tag="OO130_003469"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HCR5366740.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            complement(6920..7900)
                     /gene="btuC"
                     /locus_tag="OO130_003470"
     CDS             complement(6920..7900)
                     /gene="btuC"
                     /locus_tag="OO130_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HCR5366741.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISLGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELLIGVVTATLGAPVFIWLLLKARR"
     gene            complement(8109..8438)
                     /locus_tag="OO130_003471"
     CDS             complement(8109..8438)
                     /locus_tag="OO130_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5366742.1"
                     /translation="MNDDLERRVSCLESDVTEIKNNLITLTTRSESFATKSDVLEIRE
                     GLRLEMAESRQSLKSEMADLRQSLKVEMAEHRTELQKSFANQTWLLTGIVLSAMAVLV
                     AVVTVIK"
     gene            complement(8546..8908)
                     /locus_tag="OO130_003472"
     CDS             complement(8546..8908)
                     /locus_tag="OO130_003472"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5366743.1"
                     /translation="MKFHLDELKRMATLDNFARFLNESSTNEKCLSCGDTDMYMYLTN
                     IVEVGPEPKTAEECDLGTFVMLDYIGPFTGYPGYEGHDRENIHNYEFRLTCNRCGFVH
                     RYSARAFMNWVGKQGDEK"
     gene            complement(8911..>9046)
                     /locus_tag="OO130_003473"
     CDS             complement(8911..>9046)
                     /locus_tag="OO130_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR5366744.1"
                     /translation="MNGGNILTLQKILGHAKIQTTMIYAHLAPDYLQDAVRFNPLGGA
                     "
BASE COUNT         2392 a         2396 c         2050 g         2208 t
ORIGIN      
        1 gttgcgcaaa cctgctctat tcgacaattt ccacataaac catcggtata aacgaaacca
       61 taaaaatgaa atgctgtttt cataaaaaat aaaattgaag gttcatttta taaaccagac
      121 attacctcct acgcttctgt gcgtttttaa tttatgcttt catagaatta tgtctgcatc
      181 acgggaagaa caaaatggat aatgctgttg atcgccacgt tttttatatt tctgatggta
      241 cggcaataac tgcggaggta ttaggacacg cagtaatgtc acaatttccc gtcactatca
      301 gcagcattac gctgccgttt gtcgaaaatg agagccgtgc acgggcagtg aaggatcaga
      361 ttgacgcaat ttatcaccag acaggtgtgc gcccgctggt cttctactcc atcgtgttgc
      421 cggagattcg cgccatcatc ttgcaaagtg aaggcttttg ccaggatatc gttcaggcgc
      481 tggttgcccc gctacaacaa gagatgaaac tggatccaac gccgattgct catcgtaccc
      541 atggccttaa ccctaataat ctcaataaat atgatgcgcg cattgcggcg attgattaca
      601 cccttgccca cgatgacggc atttcgttgc gcaatctgga tcaggctcag gtgatcctgc
      661 tcggtgtttc tcgctgtggt aaaaccccca ccagtctgta tctggcaatg caatttggta
      721 tccgcacggc aaactacccc tttattgccg acgatatgga taatctggtg ctacccgcgt
      781 cgctcaaacc gcttcagcat aaattgttcg gcctgactat cgacccggaa cgtctggcgg
      841 cgattcgcga ggaacgtcgg gagaacagtc gctatgcctc gcttcgtcag tgcagaatgg
      901 aagtcgcgga agtggaagcc ttgtaccgta aaaatcagat cccgtggatt aacagtacca
      961 attattcggt agaagagatt gccaccaaga tcctcgatat catgggcctt agtcgccgaa
     1021 tgtactagag aactagtgcg ctggtttgtt ttttgttatc atgctaacca cccggcgagg
     1081 tgtgacacac ctcgcacttg aaatcagctg cgattggttt atcgtgatgc gcatcacttc
     1141 ccggtagtcc tgccgtagaa gcaacaaatt tctgagactt gtaatgaaca gaactgacga
     1201 actccgtact gcgcgtattg agagcctggt aacgcccgcc gaactcgcgc tacggtatcc
     1261 cgtaacgcct ggcgtcgcca cccatgtcac cgactcccgc cgcagaattg aaaaaatact
     1321 gaatggtgaa gataagcgac tgttggtcat tattggcccc tgctcgatcc acgatctcac
     1381 cgctgcaatg gagtacgcca cccgtctgca gtcgctgcgc aaccagtacc agtcacggct
     1441 ggaaatcgta atgcgcacct attttgaaaa accacgaact gttgtcggct ggaaagggct
     1501 aatctccgat ccggatttaa acggcagcta tcgggtaaat cacggtctgg agctggcgcg
     1561 caaattactt ttacaggtaa atgagctggg tgtccctacc gcgactgagt ttctcgatat
     1621 ggtgaccggt cagtttattg ctgatttaat cagttggggc gcgattggcg ctcgtactac
     1681 cgaaagtcag atccaccgcg aaatggcttc agcactctcc tgtccggtag gttttaaaaa
     1741 tggtaccgat ggcaacacgc ggattgccgt ggatgctatc cgcgcagccc gcaccagcca
     1801 tatgttcctc tcgccagaca aaaatggtca gatgaccatc tatcagacca gcggcaaccc
     1861 gtatggccac attattatgc gtggcggcaa aaaaccgaat tatcatgccg atgatatcgc
     1921 cgcagcctgc gatacgctgc acgagtttga tttacctgaa catctggtgg tggatttcag
     1981 ccacggtaac tgccagaagc agcaccgtcg ccagttagaa gtttgtgagg atatttgtca
     2041 gcaaatccgc aatggctcta cggcgattgc tggaattatg gcggaaagtt tcctgcgcga
     2101 aggaacgcaa aaaatcgtcg gcggtcagcc gctcacttac ggtcaatcca ttaccgaccc
     2161 gtgtctgggc tgggaagata ccgaacgcct ggtcgaaaaa ctcgcctctg cggtagatac
     2221 ccgcttctga atgcgtgccc atgcctgatg ggatgggcat ttctgcgcaa cttgttgtct
     2281 tctcaacaca ttactgcttg ctctggcctg ccataatatt gataataaga atcattgtta
     2341 tatcaattat tattaatttt tatgcgttat acggatagca gaaaactcac gcctgaaacg
     2401 gatgccaatc acaagaccgc ttccccgcag cccatccggc gaattaccag ccagacactg
     2461 ttaggtccgg atggcaaact gattatcgat catgacgggc aagaatatct gctccgtaaa
     2521 actcaggctg gcaagctgct gttaactaag tagcctttaa ctcgagcagc tgacttccag
     2581 ccgtttaccc cagtcaggtg gacgactgac atagtcatca tctctgtcgc tgaaaggatt
     2641 tcgcaacgcc tcatgcaggc ggtgcaattc catcatatca cccttttctg ccgcttcaat
     2701 cgcccgttgc gccaaccagt tgcgcaatac cagagctggg ttaacgcttt gtatcagttg
     2761 ctgacgctca ctatcgctaa cctcgtcttg ttgcaaacgc ccccgataac gggcaaacca
     2821 gtcatcaaat gccgcacgat caataaactc atcacgtagc ggtgacgccg cgctgtgctg
     2881 ctcggtcaga ctcagcatgc ggaatgtgcg ggtataatcg ctgcgctctc gcgccatcag
     2941 actgaataat tcattcagta gcgcgttatc ctctttttgc tccgtcatga agcccagttt
     3001 ctgccgcatc cgttgtccat aatgcgtcaa caaaacctgc tgatagctgt ccagggcctc
     3061 attcagggca tctacggcaa caaatggtga caatgtctgc gccagacgct gtaaattcca
     3121 caacgcgaca gcaggttgat tatcaaagct ataacgccct tgatgatccg agtgattaca
     3181 aataaaaccg ggttcgtaat catcaagaaa accaaacggc ccgtaatcaa gcgtcagtcc
     3241 cagcagcgac atgttgtcgg tattcatcac cccatgtgca aagccgaccg tctgccattg
     3301 ggcaattaac gacgcggtac gtgcgacaac atcgctaaac cagagacggt atttgtcctc
     3361 atcatcttca agatgtgacc agtaatgacg aatagcaaag tcagctaact gacgaacctt
     3421 ttccgactcg cggcggtagt aaaaatgttc gaaatgacca aagcgcagat gacttggtgc
     3481 cacacgcatc agcatcgcgc ctggctccgc cgtttcccga tacactggcg aatcgctggt
     3541 gacgatactt aacgcgcggg tcgtcggaat gcccagataa tgcatcgcct cactggcgag
     3601 actttctcgt atcgtcgaac gtaaaaccgc ccgtccatca cccattcgcg aataaggcgt
     3661 caggccagca cctttcagat gccagtccat tgtagtgcca tcagcaagca gttgttcgcc
     3721 gagtaaaatg ccgcgcccat cacccagttg gcccgcccag acgccgaact gatgaccact
     3781 gtaaacctgc gccagtggtg acataccagg cagtagggct tcgccgcccc agacacctgc
     3841 gccattttta aacagcgacg atggaatact cagcgtgtta gccagttcga tattatgcca
     3901 aatcagccgg gcattattta aaggcgtagg ggaaagtgct gtataggttt ctggcaattc
     3961 atcgcgccag cgggtaacaa aagacagggt catagatcct cctgtttgat agtgtagacg
     4021 gttactctcg tcttaaacac cagcaaacag aagggttatc gctgaaccaa cgtcgttatc
     4081 tgatttatgg gaacggcagg ccacaggcat ccctggagag catggaaatc gaagggggta
     4141 atctgtgcca atatttctgc cgtatcaatg cctcccgcaa tgatgcaatt gcaacaaggg
     4201 gaaatttgcg cctagatcgc acgaataaac ggttcgaagg agcggtgcgt gatttgttgc
     4261 tgaataaaac ttttatccag catgacgcgg gtgaaaagac catcaaaaac cgctttcatt
     4321 gtgctattgc ctgctcccaa attacctaac acaaggggat acacttgcga taaggataaa
     4381 agaccgcgat tatcttttcc ttcattgaga tgcgggtaat tctcattaat caacagttca
     4441 ataaacggat attttaatag ttcgcctgcg taattatccc tctctaataa taatgtagcc
     4501 acttgtggcg ttaaattaag ccaggcaaag agtttatgtt gaataaaaaa atgttggcat
     4561 gattttagta attccaactg ctctgaaaat aattgccaat gttgctcctc ggtaagttgc
     4621 gctataaccc gactcgtcgg gatacgcacc gtgccatctt cactggagaa atgggtaatt
     4681 aactcaacgc cgacaaggac ttgctggtta tctcttatcg gcaggaaata gcaatcagaa
     4741 tgataaagat tctccaaaaa aatcttcatg gtaaccgtcc ctctcgaagg atgtttttca
     4801 gtatccggct aaaagggata aggccataag acataacaat tacagaagga gtaactttca
     4861 tttgttccat gttaaccact ttttcagggg tcttttttta gattatcctg attataaacg
     4921 aataatccat ttacggaatt tttgtctgca aaatactact gtattcagga gtaaatggac
     4981 ggcgatgtat catcgccgtc catttttcga catactagat tcgtctcgcc tgccagaaat
     5041 ttttctgcca atagacatta tcaagtgagg aacgcatcac tcccttgctg gtagaggcgt
     5101 ggataaattg gttgttggta tcgtaaatac ctacatgcaa accattttgt ccggaccccg
     5161 ttttgaaaaa gaccaggtca cctggcagca acttgtcttt atcaatttgc gtgccaatag
     5221 aggcttgttg tttggtttca cggggcagct gcaaatcgaa acgatcgcgc atcgtcacaa
     5281 ccacaaatcc cgaacagtcc acaccgcgcc gcgtcatgcc accataacga tacggcgtgc
     5341 catgccagct ttgtagctgg tcgttcaaac cggcaataac ggtaatcgaa tcagaaagtc
     5401 tggcatttgg cggcggtgct ttatggtggc tacacccggc cagaaacagt gctgtgatca
     5461 aaataaggca gaaacgcatt ccgtacggtt cctctgtttt ttattcttgc attaatttag
     5521 cgtcgtaatt acccgatttt caagatacta atgaaatcag atggtcaaaa tcagcattct
     5581 gtgaccttcg atatccagac ggcgaaaatt catcccatag gcctgcgcca gatttggcgg
     5641 cgtgagcacc tcttctctgc gtccactggc cagcattttt ccacctttta gcaaccacgc
     5701 ccgatgcgca tgacgcaacg tgtggttgag atcgtgactg ctcatcacaa tcgccagtcc
     5761 ttgctgacac agcgcgctca gaattttgtc taacgcactt tgttgcgcaa catcaagact
     5821 gttcatcggc tcatcaagaa gcagcaattg gcctgcggga ttggcttgtg gtgtgatttg
     5881 caacaccacc gcagcaagac gtacgcgttg ccattcaccg ccggaaagtt gattggtgct
     5941 acgaccgagt ttgtcatcaa gagccagcgc ccctgcgaca tcattcagta gttcggtacg
     6001 cgttttatcg tgctgatgca gtgtcaggta gtgccagacc ggcattgcaa acggcggcgt
     6061 ctgctgttgt gaaagatagg cgcgatgcag cgcgagtttt gttgcggacc atgcttccag
     6121 gggttgcccc gcgaactgaa tgcttccctt accgctggtc attccggcca ttcgtgcaag
     6181 caaggtactc ttacccgcgc cattcggccc caccaggtgc aggatctccc cagcccgaac
     6241 ctcgccagaa agtggcccca ggcgggtaga ttccgcaaca tcctgtaact gcatcacaat
     6301 agacattatt ttgccaacgc cagtttaatg ctttccatca caatggggtc ttccggcgtc
     6361 atatccgggg aaaaacgctg gatgaccaat ccgtccctgc caactaaaaa tttctcaaaa
     6421 ttccataaaa tatcatcagg gtacagcggt gcacgacctt tgctgaccat acgggcatag
     6481 aatccgctct cttccggcgc gactgcggtc ggcgctgcgg caatcaattt ttgatacagc
     6541 ggatggcgtc cttcgccgtt aacttcaatc ttactgaaca tcgggaacgt caccccccat
     6601 gtggtggtac agtaagtttt aatctcttca tcgctgcccg gttcttgttc cagaaactgg
     6661 ttgcacggga atcccagcac cacaaaacct cgatcggccc aggctttctg aatattctcc
     6721 aactgctcat attgcggcgt taagccacac tttgaggcga cattgacaat caacagcaca
     6781 ttaccggcgt acttctccag cgtggtcact tcaccgtcga tatctttcac tacggtcgtc
     6841 agaatggaat cttgcatcgt ttctcctggg tgtggtcagt aaaaatctta gcttttaatc
     6901 atagaccgtc tttttgcggc taacgtcttg cttttaacaa taaccagata aacaccggcg
     6961 cacccaacgt tgcggtgacc acgccaataa gcagctctgc ggcagctaat gccaggcgcg
     7021 ctacaatatc ggccagcagc aatgcgctcg cccctgccag cgcgcagccg ggaagtaata
     7081 cgcgatgatc ggttaaacca cacaaccgaa gaatatgtgg gatcaccaga ccaataaagc
     7141 cgatagcacc cgccagcgcc acactgacgc caaccatcca gccggtcgcg gccaccagca
     7201 cattgcgcca gaaccacagg ggtaaaccca gttgccgcgc cgagatctcg ccaagtgcta
     7261 acatattcat cggcctggac tgacaacaga tccacaacaa cacggggatc aatgccagca
     7321 tcagccagct ttgccgccag tctacgccgc caaaaccgcc catcatccag tacatcagct
     7381 gacgtaaatc aacagaggtg gaaaagtaga tagcccacgt cattagtgcg ctacagataa
     7441 tccctaatgc aacgccagcc agcaataacc gactggtcga aagatgacga cgggcgaaac
     7501 gtaagagtat taaagtaatg ataagcgcgc cagcaatcgc acacagccct agcgcccagt
     7561 tggggagttg cccttgccca agcaataccg cggcgataag ccccacgcct gcgccgttag
     7621 agacgccaag tagtccaggt tctgccagag gattttcaaa caacgcctgc attacagcgc
     7681 cggatatagc cagcgccgca ccaaccaaca atacagccag cgtacgtggc aggcgaattt
     7741 gccagacgaa cagttcgcca cgaggagtaa accagtcacc tagcgagatc cattgttcac
     7801 cggcgcaaag gcttaaaaga agcgccagca gcatcaaaac tgacaggcat aataaccagc
     7861 gaatattttg tcgctgttgt tggcgggcaa gtgtcagcat ggtatccgtt ctgctgaagt
     7921 gtcatggcgt tgattttacg gtgactcttc gacagtgaaa agaaaaaagg ccgcagagcg
     7981 gccataaaca caagtaaaaa tcaacaagtt agataattat caaagactta cagacacaca
     8041 aaaacacagc caaccacaac aaataacagg gatgtggtca ctttgtggat catatcgccg
     8101 ccataaattt acttaataac agtaaccaca gccacaagca cggccatcgc agacaggaca
     8161 atacctgtga gtaaccaagt ctgatttgca aatgatttct gaagttcagt acgatgctca
     8221 gccatctcaa ctttcagaga ctggcgtaaa tcagccatct cagacttcag ggattggcgc
     8281 gattctgcca tctcaagcct caacccttcg cgaatctcca gtacatcaga tttggtagca
     8341 aaggattcgc ttcttgtggt gagagtaatc aggttgtttt tgatttcagt cacatcactt
     8401 tccaggcaac tgacccgccg ttcaaggtcg tcattcatgc catcacctcc atcattacca
     8461 cttcctgtta cttggttgtt ggatacatta taggcattct ctttcttttt tctgaaccgc
     8521 gaaatatgtg caacattctc tgccatcact tctcatcacc ctgcttgcct acccagttca
     8581 taaaggcccg ggccgagtaa cgatgaacaa acccacatct gttacaggta agtcggaatt
     8641 catagttatg gatattttcc cgatcatgtc cctcatatcc cggataccca gtaaatggac
     8701 cgatgtaatc aagcataaca aaagtgccta aatcacactc ctcagcagtt ttcggctcag
     8761 gccccacctc aacaatattc gtcaaataca tgtacatatc cgtatcgcca cacgacaaac
     8821 atttttcatt cgtagacgac tcattgagaa aacgcgcaaa attatcgagc gttgccattc
     8881 ttttaagttc atctaaatga aacttcactc ttaagcacct cctagtggat taaacctcac
     8941 cgcatcctgc aggtaatccg gcgcaagatg ggcataaatc atcgttgtct gaatctttgc
     9001 gtgccccaga attttctgga gcgtcagaat attgccgccg ttcatc
//
LOCUS       DAJTMC010000165         8734 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.165, whole genome shotgun sequence.
ACCESSION   DAJTMC010000165 DAJTMC010000000
VERSION     DAJTMC010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8734
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.165"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            244..1155
                     /gene="ttdA"
                     /locus_tag="OO130_003474"
     CDS             244..1155
                     /gene="ttdA"
                     /locus_tag="OO130_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HCR5366745.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIIPDNDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLTFENLSHTRSAL"
     gene            1152..1757
                     /gene="ttdB"
                     /locus_tag="OO130_003475"
     CDS             1152..1757
                     /gene="ttdB"
                     /locus_tag="OO130_003475"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HCR5366746.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRGKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            1806..3269
                     /gene="ttdT"
                     /locus_tag="OO130_003476"
     CDS             1806..3269
                     /gene="ttdT"
                     /locus_tag="OO130_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HCR5366747.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALIPVPAGLESHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVFGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(3312..4325)
                     /gene="tsaD"
                     /locus_tag="OO130_003477"
     CDS             complement(3312..4325)
                     /gene="tsaD"
                     /locus_tag="OO130_003477"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HCR5366748.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            4562..4777
                     /gene="rpsU"
                     /locus_tag="OO130_003478"
     CDS             4562..4777
                     /gene="rpsU"
                     /locus_tag="OO130_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HCR5366749.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            4888..6633
                     /gene="dnaG"
                     /locus_tag="OO130_003479"
     CDS             4888..6633
                     /gene="dnaG"
                     /locus_tag="OO130_003479"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCR5366750.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            6828..8669
                     /gene="rpoD"
                     /locus_tag="OO130_003480"
     CDS             6828..8669
                     /gene="rpoD"
                     /locus_tag="OO130_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HCR5366751.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
BASE COUNT         2109 a         2330 c         2334 g         1961 t
ORIGIN      
        1 cctgcaagtc tttggctaaa ggccagctat tcagcatcgt taattatccg cagttgtgat
       61 aagcgcagtg tatttcgcaa aacattgcca ttccctgctc tggcgcaata ttcattctta
      121 ttttcgcgtt gattcacgaa ctgttaatta catattcacg gtggcaaaaa atataaaacc
      181 acatttttag tggtagttta tggcggtgaa tttttccaga caaatacaaa aactggagtt
      241 gccatgatga gcgaaagtaa taagcaacag gcagtgaata agttgacaga gattgtcgct
      301 aactttaccg ccatgatttc tacccgaatg cctgatgacg tggtggataa actaaaacag
      361 ctaaaggatg ccgaaacgtc gtcgatgggg aaaattatct accatacgat gttcgacaac
      421 atgcaaaaag cgattgacct gaatcgtcct gcctgtcagg acaccgggga gattatgttc
      481 ttcgttaaag tcggttcccg cttcccactg cttggcgagc tgcaaagcat actcaaacaa
      541 gccgtggaag aggcaaccgt caaagcgcca ctacgtcaca atgcggtaga aatttttgac
      601 gaagtaaaca ccggcaaaaa taccggtagc ggcgtaccgt gggtcacctg ggacatcatc
      661 cccgacaatg acgatgcgga aatcgaagtt tacatggcag gtggcggctg cacgctacct
      721 ggccgctcga aagtgttaat gccgtcagaa ggctacgaag gcgtggtgaa attcgtcttc
      781 gaaaatatct ccaccctcgc cgtaaacgcc tgtccaccgg tactggtggg cgtgggcatc
      841 gccacctcgg tggaaaccgc cgccgtactc tcgcgtaaag ccattttgcg cccgattggc
      901 agccgccatc ccaatccaaa agcggcagaa ctggagctac gcctggaaga aggactcaac
      961 cgtctgggga ttggtccaca agggctgact ggcaacagtt cagtgatggg cgtacatatc
     1021 gaatctgccg cccgccatcc gtcaaccatc ggcgttgctg tctctaccgg ctgctgggcg
     1081 catcgtcgcg gcacactgct ggttcatgcc gatctcacct ttgaaaatct gtctcacacc
     1141 cggagcgcgt tatgaaaaag atcctgacaa ccccgatcaa agctgaagat ctgcaagata
     1201 ttcgcgtcgg cgatgtgatc tacctgaccg gtacgctggt gacctgccgt gacgtttgtc
     1261 accgccgctt gatcgaactg aaacgtccga tcccttacga tctcaacggc aaagcgattt
     1321 tccacgctgg ccccatcgtg cgcaaaaacg gcgataaatg ggagatggtt tccgttggcc
     1381 cgacaaccag tatgcgtatg gaaagttttg aacgtgaatt tatcgagcag accggcgtga
     1441 aactggtggt tggcaaaggc ggaatggggc cgctgaccga agaaggctgc cagaaattca
     1501 aggcgctaca tgtgattttc ccggcaggct gcgcggtgct ggcggcaacc caggtggaag
     1561 agattgaaga agtgcactgg acagagctcg gaatgccgga gtcgctgtgg gtctgccggg
     1621 gcaaagagtt cggcccgctg attgtctcta ttgataccca cggcaacaat ctgatagccg
     1681 aaaacaaaaa gctgttcgcc gaacgccgcg atcccatcgt ggaagagatc tgcgagcacg
     1741 tccattacat caaataaccc tcccggagag gctcgccccc tctccttttt cgcaggcata
     1801 acacgatgaa accttccact gaatggtggc gataccttgc gccgctggcg gtcatcgcca
     1861 ttattgctct aattccggtt cccgcagggc tggagagtca tacctggctc tactttgccg
     1921 tttttactgg cgtgatcgtt ggactgatcc tcgaacccgt gccgggtgcc gtggtggcga
     1981 tggtgggtat ctccatcatc gccatactct ctccctggct gctgttcagc cccgaacagc
     2041 tcgcccagcc aggctttaag ttcaccgcca aatccctctc ctgggcagtt tttggttttt
     2101 ctaattcggt tatctggctc attttcgccg cctttatgtt tggcacaggc tatgaaaaaa
     2161 ccgggcttgg acgccgcatt gcgctgattc tggtgaaaaa gatgggacat cgcacgctgt
     2221 ttctcggcta tgcggtgatg ttctccgagc tgatcctcgc acctgtaaca ccgtccaact
     2281 cggcgcgtgg tgcggggatt atctatccca tcatccgtaa cctgccaccg ctctatcaat
     2341 cacaaccaaa cgacagcagt tcgcgcagca ttggctcgta catcatgtgg atggggattg
     2401 ttgccgactg cgtgaccagc gccattttct tgacggcgat ggcaccaaac ttgctgttaa
     2461 ttggactgat gaaaagcgca tctcacgcca cgctgagttg gggcgactgg ttcctcggga
     2521 tgttgccgct cagtattttg ctggtgctgc tggttccctg gctggcttat gtgctgtacc
     2581 cgccggtact gaagtctggc gatcaggtgc cgcgctgggc agagacggaa ctgcaggcaa
     2641 tgggcccgct ctgttcgcgt gaaaaacgga tgctggggct gatggtaggc gcgctggtgc
     2701 tgtggatttt cggcggtgat tatatcgatg ccgcgatggt cggttacagc gtggtggcac
     2761 tgatgctgct tctgcgcatt atcagttggg acgacattgt cagtaataaa gcggcgtgga
     2821 acgttttctt ctggctggcc tcgcttatca ccctcgctac cggactcaac aacactggtt
     2881 ttattagctg gtttggcaaa ctgttagcag gcagcttaag cggttattcg ccaacgatgg
     2941 tgatggtggc gttgattgtg gtgttttatc tgctgcgcta ctttttcgcc agcgccacgg
     3001 cgtatacctc cgccctcgca ccgatgatga ttgccgctgc gctggcgatg ccggaaatcc
     3061 cgctgccggt gttctgccta atggttggcg cggcaattgg tctggggagc attcttacac
     3121 catacgccac cggacccagt ccgatttact acggtagtgg ttatctgcca acggcggatt
     3181 actggcgact gggggcgatt tttgggctga tattcctcgt gttgctggtg attaccggct
     3241 tactgtggat gcccgtggtg ttgctttaag tgaggacggt gccggaaatt gctctccggc
     3301 acccaatagt tttacgcagc cggtaactcc gccagcggcc agcgcggacg cacgctaacg
     3361 ccgagatccg ccgtcgcgcc tgctttaaac cgcaccattc cggcataggc gatcatcgcg
     3421 ccgttatcag tacaaaactc cggacgcgcg tagaacactt cgccgcggcg ttttttcatc
     3481 atttccgcca gcttcgcccg taacgtgcgg ttagcactca cgccgcccgc catgaccagt
     3541 cgcttaaagc ccgtctgatc caacgctcgc ttacacttaa tcatcaacgt atcgaccacc
     3601 gcatcttcaa aggcgcgggc gatatcagca cgcgtctggt cgtcggtgcc gttgtcacga
     3661 atcgtgttcg ccgcaaaggt tttcagacca gaaaagctga aatccagccc cggacggtcg
     3721 gtcatcggac gcgggaaaac aaagcgcccg gcagtaccct gagccgccat tttcgacagt
     3781 aacggtccgc caggataatc cagccccagc agcttcgcgg ttttatcaaa cgcttcaccg
     3841 gcggcatcat cgatagactc gccgagcagc tcgtactgac caatgccagt cacgctgatt
     3901 aactgcgtat gaccgccgga caccagcagc gcgacaaacg gaaattccgg cgggttatct
     3961 tccagcatcg gcgctaacag atgcccttcc atatggtgta ccgggattgc cggaacgttc
     4021 caggcaaacg ccagagaacg ccccacggtt gcgccaacca gcagtgcgcc gactaatcca
     4081 gggcctgcgg tataggccac agcatcaatg tcttttgccg ttaagccaga ctcctttagc
     4141 gccgcctgga tcaacggtac ggttttgcgc acgtgatcgc gggaggccag ttcaggcacg
     4201 acgccgccgt agtcagcgtg caatttcacc tgactataca attggttggc taacaaacct
     4261 ttttcatcgt cgtaaatggc gatgccggtt tcatcgcagg aagtttcaat acccagtaca
     4321 cgcatgactt gttttacctc gctttattac cgcgcagtgt aggaccaatg cgggttgatg
     4381 taaaactttg ttcgcccctg gagaaagcct cgtgtatact cctcaccctt ataaaagtcc
     4441 ctttcaaaaa ggccgcggtg ctttacaaag cagcagcaat tgcagtaaaa ttccgcacca
     4501 ttttgaaata agctggcgtt gatgccagcg gcaaaccgaa ttaatcaaag gtgagaggca
     4561 catgccggta attaaagtac gtgaaaacga gccgttcgac gtagctctgc gtcgcttcaa
     4621 gcgttcctgc gaaaaagcag gtgttctggc ggaagttcgt cgtcgtgagt tctatgaaaa
     4681 accgactacc gaacgtaagc gcgctaaagc ttctgcagtg aaacgtcacg cgaagaaact
     4741 ggctcgcgaa aacgcacgcc gcactcgtct gtactaattc cccgagagcg ttgctctccg
     4801 atcagaccga gttgtagttg taaggccgtg cttccgaaag gaatgcgcgg cttattttcg
     4861 tttatgaatt gctaaaaatc ggggcctatg gctggacgaa tcccacgcgt attcattaat
     4921 gatctactgg cacgcactga catcgtcgat ctgatcgatg cccgtgtgaa gctgaaaaag
     4981 cagggcaaga atttccacgc gtgttgtcca ttccacaacg agaaaacccc gtcattcacc
     5041 gttaacggtg agaaacagtt ttaccactgc tttggatgtg gcgcgcacgg caacgcgatc
     5101 gacttcctga tgaactacga caagcttgag ttcgtcgaaa cggtcgaaga gctggcagca
     5161 atgcacaatc ttgaagtgcc atttgaagca ggcagcggcc ccagccagat cgagcgccat
     5221 cagaggcaaa cgctttatca gttgatggac ggtctgaata cgttttacca acaatcttta
     5281 caacaacctg ttgccacgtc tgcgcgccag tatctggaaa aacgcggatt aagccacgag
     5341 gttatcgctc gctttgcgat tggttttgcg ccccccggct gggacaacgt cctgaagcgg
     5401 tttggcggca atccagaaaa tcgccagtca ttgattgatg cggggatgtt ggtcactaac
     5461 gatcagggac gcagttacga tcgtttccgc gagcgggtga tgttccccat tcgcgataaa
     5521 cgcggtcggg tgattggttt tggcgggcgc gtgctgggca acgatacccc caaatacctg
     5581 aactcgccgg aaacagacat tttccataaa ggccgccagc tttacggtct ttatgaagcg
     5641 cagcaggata acgctgaacc caatcgtctg cttgtggtcg aaggctatat ggacgtggtg
     5701 gcgctggcgc aatacggcat taattacgcc gttgcgtcgt taggtacgtc aaccaccgcc
     5761 gatcacatac aactgttgtt ccgcgcgacc aacaatgtca tttgctgtta tgacggcgac
     5821 cgtgcaggcc gtgatgccgc atggcgagcg ctggaaacgg cgctgcctta catgacagac
     5881 ggccgtcagc tacgctttat gtttttgccg gatggcgaag accctgacac gctggtacga
     5941 aaagaaggta aagaagcgtt tgaagcgcgg atggagcagg cgatgccact ctccgcattt
     6001 ctgtttaaca gcctgatgcc gcaagttgat ctgagtaccc ctgacgggcg cgcacgtttg
     6061 agtacgctgg cactgccatt gatatcgcaa gtgccgggcg aaacgctgcg aatatatctt
     6121 cgtcaggaat taggcaacaa attaggcata cttgatgaca gccagcttga acgattaatg
     6181 ccaaaagcgg cagagagcgg cgtttctcgc cctgttccgc agctaaaacg cacgaccatg
     6241 cgtatactta tagggttgct ggtgcaaaat ccagaattag cgacgttggt cccgccgctt
     6301 gagaatctgg atgaaaataa gctccctgga cttggcttat tcagagaact ggtcaacact
     6361 tgtctctccc agccaggtct gaccaccggg caacttttag agcactatcg tggtacaaat
     6421 aatgctgcca cccttgaaaa actgtcgatg tgggacgata tagcagataa gaatattgct
     6481 gagcaaacct tcaccgactc actcaaccat atgtttgatt cgctgcttga actgcgccag
     6541 gaagagttaa tcgctcgtga gcgcacgcat ggtttaagca acgaagaacg cctggagctc
     6601 tggacattaa accaggagct ggcgaaaaag tgatttaacg gcttaagtgc cgaagagcga
     6661 tcgggaagcc cccgacagcc gcactgagag gcagcggcaa atatataagt acgccctcgt
     6721 aattatcgtt ggcggtaaac aaccgttgga tttcagcgtt aacggctgaa ggacatcggg
     6781 tcaatcgccc aacaccaacc tcatgaaata agtgtggata ccgtcttatg gagcaaaacc
     6841 cgcagtcaca gctgaaactt cttgtcaccc gtggtaagga gcaaggctat ctgacctatg
     6901 ccgaggtcaa tgaccatctg ccggaagata tcgtcgattc agatcagatc gaagacatca
     6961 tccaaatgat caacgacatg ggcattcagg tgatggaaga agcaccggat gccgatgatc
     7021 tgatgctggc tgaaaacacc gcggacgaag atgctgccga agccgccgcg caggtgcttt
     7081 ccagcgtgga atctgaaatc gggcgcacga ctgacccggt acgcatgtac atgcgtgaaa
     7141 tgggcaccgt tgaactgttg acccgcgaag gcgaaattga catcgctaag cgtattgaag
     7201 acgggatcaa ccaggttcaa tgctccgttg ctgaatatcc ggaagcgatc acctatctgc
     7261 tggaacagta cgatcgtgtt gaagcagaag aagcgcgtct gtccgatctg atcaccggct
     7321 ttgttgaccc gaacgcagaa gaagatctgg cacctaccgc cactcacgtc ggttctgagc
     7381 tttcccagga agatctggac gatgacgaag atgaagacga agaagatggc gatgacgaca
     7441 gcgccgatga tgacaacagc atcgacccgg aactggctcg cgaaaaattt gcggaactac
     7501 gcgctcagta cgttgtaacg cgtgacacca tcaaagcgaa aggtcgcagt cacgctgccg
     7561 ctcaggaaga gatcctgaaa ctgtctgaag tattcaaaca gttccgcctg gtgccgaagc
     7621 agtttgacta cctggtcaac agcatgcgcg tcatgatgga ccgcgttcgt acgcaagaac
     7681 gtctgatcat gaagctctgc gttgagcagt gcaaaatgcc gaagaaaaac ttcattaccc
     7741 tgtttaccgg caacgaaacc agcgatacct ggttcaacgc ggcaattgcg atgaacaagc
     7801 cgtggtcgga aaaactgcac gatgtctctg aagaagtgca tcgcgccctg cagaaactgc
     7861 agcagattga agaagaaacc ggcctgacca tcgagcaggt taaagatatc aaccgtcgta
     7921 tgtccatcgg tgaagcgaaa gcccgccgtg cgaagaaaga gatggttgaa gcgaacttac
     7981 gtctggttat ttctatcgcc aagaaataca ccaaccgtgg cttgcagttc cttgacctga
     8041 ttcaggaagg caacatcggt ctgatgaaag cggttgataa attcgaatac cgccgtggtt
     8101 acaagttctc cacctacgca acctggtgga tccgtcaggc gatcacccgt tctatcgcgg
     8161 atcaggcgcg caccatccgt attccggtgc atatgattga gactatcaac aaactcaacc
     8221 gtatttctcg ccagatgctg caagagatgg gccgtgaacc gacgccggaa gaactggctg
     8281 aacgtatgct gatgccggaa gacaagatcc gcaaagtgct gaagatcgcc aaagagccaa
     8341 tctccatgga aacgccgatc ggtgatgatg aagattcgca tctgggggat ttcatcgagg
     8401 ataccaccct cgagctgccg ctggattctg cgaccaccga aagcctgcgt gcggcaacgc
     8461 acgacgtgct ggctggcctg accgcgcgtg aagcgaaagt tctgcgtatg cgtttcggta
     8521 tcgatatgaa caccgaccac acgctggaag aagtgggtaa acagttcgac gttacccgcg
     8581 aacgtatccg tcagatcgaa gcgaaggcgc tgcgcaaact gcgtcacccg agccgttctg
     8641 aagtgttgcg tagcttcctg gacgattaat cggtaggccg gatcaggcgt tacgccgcac
     8701 ccggcactag tccctctgca caaacgccac cttt
//
LOCUS       DAJTMC010000166         8723 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.166, whole genome shotgun sequence.
ACCESSION   DAJTMC010000166 DAJTMC010000000
VERSION     DAJTMC010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8723
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.166"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            134..199
                     /gene="yobI"
                     /locus_tag="OO130_003481"
     CDS             134..199
                     /gene="yobI"
                     /locus_tag="OO130_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HCR5366752.1"
                     /translation="MYIFITHFFTEYVIFKYLLPI"
     gene            338..949
                     /gene="ruvA"
                     /locus_tag="OO130_003482"
     CDS             338..949
                     /gene="ruvA"
                     /locus_tag="OO130_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HCR5366753.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
     gene            958..1968
                     /gene="ruvB"
                     /locus_tag="OO130_003483"
     CDS             958..1968
                     /gene="ruvB"
                     /locus_tag="OO130_003483"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HCR5366754.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(2018..2803)
                     /gene="znuB"
                     /locus_tag="OO130_003484"
     CDS             complement(2018..2803)
                     /gene="znuB"
                     /locus_tag="OO130_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HCR5366755.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLALDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLIAIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(2800..3555)
                     /gene="znuC"
                     /locus_tag="OO130_003485"
     CDS             complement(2800..3555)
                     /gene="znuC"
                     /locus_tag="OO130_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HCR5366756.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            3634..4566
                     /gene="znuA"
                     /locus_tag="OO130_003486"
     CDS             3634..4566
                     /gene="znuA"
                     /locus_tag="OO130_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HCR5366757.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLLGAKQV
                     TIAQLEDVKLLLMKSIHGDDDDHDHAEKSEEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            4582..5904
                     /gene="mepM"
                     /locus_tag="OO130_003487"
     CDS             4582..5904
                     /gene="mepM"
                     /locus_tag="OO130_003487"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HCR5366758.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            6024..6995
                     /gene="lpxM"
                     /locus_tag="OO130_003488"
                     /gene_synonym="msbB"
     CDS             6024..6995
                     /gene="lpxM"
                     /locus_tag="OO130_003488"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCR5366759.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMVGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLQIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRRPGEIQPYKRKDLYPIK"
     gene            complement(7084..>8723)
                     /locus_tag="OO130_003489"
     CDS             complement(7084..>8723)
                     /locus_tag="OO130_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HCR5366760.1"
                     /translation="DGLPHVDFSRVDKFFTYADAFRESLPIISGELYFEAHQGCFTSE
                     SATKAHNRNMENKLHDAEFFTTITNNMTSILRSEFDEIWKEMLTLQFHDILPGSCISR
                     VYLETEKEYIKLEAKTEKIISDTQSTLLSKIDTSSYKDPHILFNTTCFARNEWININN
                     NWLKARVNSYGYAVIDPKNKIVNGLKAESRSIENNYIKLLFSENGDLISLYDKRYGKE
                     YITENMHSEIRAYHEDAGFFAAWDFASNYRDGESYVLLAEKMTTVISGPKTTMTLIYH
                     YNSSYLRFAFTLTQDSPLVDVQTFIDWHEPNVSLKVKFPVSVQTSLAQCQIQFGVIDR
                     PTHSDDSFAFAKDEIPAHHWVDLSDRETGIALIAKNKYGYRVKDQTLKLTLLRSQHKP
                     GEVVKTDNYDNFLINNFADIGKQKFIFSLFPHHGDYRVGGVINQAYIMNHPLQVVPVK
                     PHPGELPPSLSFFAIDTNSVIIETIKLAEDGDGIIVRLYESHGLEISAMLSWVCNYHY
                     VQYVDLQENKLDDSFYCNQYCNLEFKPFEIITLRLTQ"
BASE COUNT         2378 a         2011 c         2127 g         2207 t
ORIGIN      
        1 agcacctata catgtaaaga cgatatctcc aatttcatat tcagcagggt aattaatatt
       61 cacgttgtca ttccattgaa atagatacga atatacgtaa tagcataaaa aaattatttt
      121 ttcaggaggt gcaatgtata tttttattac gcatttcttc actgaatacg taatatttaa
      181 atatttgctt ccaatataac ctgtagaata aatcatactg cgccattttt cagttcatcg
      241 agacacctcg caagttttct tcatccttcg ctggatatct atccagcatt tttttatcat
      301 acagcattat ctttgattca ttacgcagga gcgtcatgtg ataggcagac tcagaggcat
      361 catcattgaa aaacaacccc cgctggtgtt aattgaagtg ggcggcgtag gctatgaagt
      421 gcatatgccg atgacctgtt tttatgaact ccctgaagcg ggtcaggaag cgatcgtttt
      481 cacccacttt gtggtgcgtg aagacgcgca actgctgtac ggttttaaca ataaacaaga
      541 gcgcacattg ttcaaagagt tgatcaaaac caacggcgtc ggcccgaagt tggcgctggc
      601 gatcctctcc ggaatgtcag cgcagcagtt cgttaatgcc gttgaacgtg aagaagtggg
      661 ggcactggtg aaactgccgg gtattggcaa aaaaaccgcc gaacgcttga ttgttgaaat
      721 gaaagaccga tttaaaggtt tgcatggcga tctgtttacc cctgccgccg acctggtact
      781 cacatcacct gccagcccgg cgaccgacga tgctgaacag gaagcggttg ccgcgctggt
      841 ggcgctgggc tataaaccac aagaagcaag ccgcatggtg agcaaaatcg ctcgccctga
      901 cgccagcagt gaaacattaa ttcgcgaagc cctacgcgcc gcgttatgag gtaaaggatg
      961 attgaagcag accgtctgat ttctgccggt accactttgc cggaagatgt agcagatcgc
     1021 gccattcgcc ctaaattact ggaagagtat gttggtcagc cgcaggttcg ttcacagatg
     1081 gagattttca tcaaagcagc gaaactgcgc ggcgatgccc tcgatcattt gttgattttt
     1141 ggtcctccgg ggttgggtaa aactacgctt gccaacattg tcgccaatga aatgggcgtt
     1201 aatttacgca cgacttctgg tccggtgctg gaaaaggcgg gcgatctggc agcgatgctc
     1261 actaaccttg aaccgcatga cgtgctgttt attgatgaga tccaccgttt atcgccagtt
     1321 gttgaagaag tgctgtaccc ggcaatggaa gactaccaac tggatatcat gattggtgaa
     1381 ggtccggcgg cacgctccat taaaattgat ttgccgccgt ttaccctgat tggcgcaacc
     1441 acgcgcgcag gttcgctgac atcaccgttg cgcgaccgtt ttggtattgt gcaacgtctg
     1501 gagttttatc aggtgccgga tctgcaatat atcgttagtc gcagtgcacg ctttatgggg
     1561 cttgagatga gtgatgacgg cgcgctggaa gttgctcgtc gcgctcgcgg tacgccacgt
     1621 attgccaacc gtctgctgcg tcgagtgcgt gatttcgccg aagtgaagca cgatggcacc
     1681 atctcggcag atatcgctgc tcaggcgctg gacatgttga atgtcgatgc tgaaggtttc
     1741 gattatatgg accgcaaatt gttgctggcg gtaatcgata agttctttgg tgggccggta
     1801 ggtctggata acctggcggc ggctattggc gaagaacgtg aaaccattga ggatgtgctg
     1861 gaaccttatt tgattcagca aggctttttg cagcgtacac cgcgtgggcg tatggcgacg
     1921 acgcgggcgt ggaatcactt tggcataacg ccgccagaaa tgccgtaagt cggattggtc
     1981 gctcgcgccg catccgacaa atgtgttcag cgatagatta gctggcctgc tttttcatca
     2041 tactgataat aaataacagt gctgcacata gcaccaccga cggacccgcc ggcgtatcgt
     2101 aaaatgcgga aaaggttaaa ccgcccgtta cggcaaccat ccccaccaac acagcgacac
     2161 ctgccatctg ttccggcgtg cgggcaaagc gacgtgcagt agcagcagga ataatcagca
     2221 gtgaagtaat aatcaacgcg ccaacaaatt tcatcgctac accaatcgtc aatgccgtca
     2281 ccagcatcaa caacaatttc acgcgctgta atttcacacc atcaacaaac gccagatccg
     2341 ggctaatcgt catcgacagc aaattgcgcc attgccagaa caaaatagcc accacgatga
     2401 ccacgccaat cgcaatagcg atgagatctt ctggcgtcac tgccagcaaa tcaccgaaca
     2461 ggtaagccat caaatcaaca cgaatgttag acatcagact aacgaccacc aggcccagcg
     2521 acagggcact gtgcgccata atccctaata acgtgtcaag cgccagctgt ggacgtttct
     2581 ccagccatac cagaccgccc gccaggagca gcgtaacggc aattaccgca tagaatggat
     2641 tcacgtccag caacaaacca aacgcgacgc caagtaatga ggcatgagcc agcgtatcac
     2701 cgaaataaga catacgacgc cagactacaa acgaacccag cggacccgcg gcacaggcga
     2761 gcatgatccc ggctaaccaa ccgggaaata ataattcaat catgagcgat catttccccg
     2821 acgcaaaaca attcgtccct gtaaatcgtg acgatgatta tgatgatggc gatagatacc
     2881 cagttgttca gcaccacgag gaccaaacat agaaataaac tccggatgta gggaaacaac
     2941 ttccggtgtg ccggaacaac aaatgtggtg attcagacac agcacttcat cggtttttgc
     3001 cattaccaga tgcagatcgt gagagaccat taaaacgcca caatccagtt cgcgacgcaa
     3061 ctggtcaata aggtcatata acgccacctg gccatttaca tccacgcctt gagtgggttc
     3121 atccagcact aataattgcg gtcgatttaa caatgctcgc gctaacagta cacgctgtgt
     3181 ttcgccacca gagagctttt gcatcggtgc gttaatcaga tgcccggcct ggacacgttt
     3241 cagtgcaggc aaaatatctt ctttatgtgt gccagggcgt aagcgtaaaa aacggtttac
     3301 ggtcagtggc aacgtggtgt cgagatacag cttctgcggt acatagccga tgcgtagttt
     3361 tccgttgcgc ttgataaccc cttcatcggg tgttaccagc ccgagcacta cccgtaccag
     3421 tgtcgactta cctgcgccat ttggcccaag taaagtcaaa atttttccag gtttaagttc
     3481 cagcgacaca tcagagagga cgcggcgttg gccaaaagaa accgagacat tttccaggga
     3541 aaccagactt gtcatgttaa ttttagtctt gcagtagtca tgagatgtta taatatcaca
     3601 cttctcatat tcattacgat gattggtcgc attatgttac ataaaaaaac gcttcttttc
     3661 gcagcattat ccgccgctct ctggggaggt gcaacacagg ccgcagatgc cgccgttgtc
     3721 gcttcgctta aacccgttgg gttcatcgct tctgccattg ctgatggtgt aacagaaacg
     3781 gaggttttac tgcctgacgg cgcttcagaa catgattatt cactgcgtcc atcggatgta
     3841 aaacgcttac agaacgcgga cttagtcgtt tgggttggcc cggagatgga agcgtttatg
     3901 caaaaaccgg taagcaaatt actaggagcg aagcaggtaa cgattgcgca gcttgaagat
     3961 gtgaaactgc tactgatgaa aagtattcac ggcgatgatg atgatcacga ccacgcggaa
     4021 aaaagtgaag aagatcacca tcacggcgat ttcaacatgc atctttggct ttccccagag
     4081 atagcgcggg ctacagcggt tgcaatccat ggaaaattag tggaacttat gccgcaaagt
     4141 cgagccaaac ttgacgccaa cctgaaggat tttgaggcac aattagcctc aaccgaaacg
     4201 caggttggta acgagctcgc gccgctcaag gggaaaggtt atttcgtttt tcacgatgct
     4261 tacggctatt tcgaaaaaca gttcggactg acaccgcttg gtcattttac cgttaaccct
     4321 gagattcaac ctggcgcgca gcgtttacat gaaataagaa cacagttggt tgagcaaaaa
     4381 gcaacctgcg tttttgctga gccacagttc aggccagcgg tcgttgagag cgtcgcacga
     4441 gggacatccg ttcgtatggg aacgttggat cccttgggga cgaatatcaa actgggtaaa
     4501 acaagctatt cagaattcct gagtcaatta gccaaccagt atgcgagctg cctgaaagga
     4561 gattaatgag gaagtgatta cgtgcaacag atagcccgct ctgtcgccct ggcgtttaat
     4621 aatttaccgc gaccacaccg cgttatgttg gggtcgctca ccgttcttac tctggccgtc
     4681 gctgtctggc ggccctatgt ttatcaccgc gatgccacgc caattgtcaa aaccattgag
     4741 ctggaacaga acgaaattcg ttcgctctta cctgaagcca gtgagccgat tgatcaagct
     4801 gcacaagaag atgaagccat tccccaggac gaactggatg acaaaatcgc cggtgaagcg
     4861 ggcgtgcatg aatatgttgt ttccactggc gatacgctaa gcagcattct caatcagtat
     4921 ggtattgata tgggtgatat cacccaactg gctgcggccg acaaagaatt gcgtaacctg
     4981 aaaatcggtc aacaactctc ctggacatta accgcggacg gcgaactgca gcgcctcacc
     5041 tgggaagtgt ctcgtcgtga aacccgaacc tatgaccgta ctgccgctaa cggttttaaa
     5101 atgaccagcg aaatgcagca aggagagtgg gttaacaatc tgctgaaagg taccgtcgga
     5161 gggagctttg ttgccagcgc cagaaacgcc ggtttaacca gcgccgaagt gagcgcagtg
     5221 attaaagcca tgcagtggca aatggatttc cgcaaactga aaaaaggcga tgaatttgcg
     5281 gtgttaatgt ctcgagagat gcttgatggt aaacgtgagc aaagccagct gctgggcgta
     5341 cgtttgcgtt cagaaggtaa agattattac gcaatccgcg ctgaggatgg caaattctac
     5401 gaccgtaacg gtactggtct ggcgaaagga ttcttgcgat tcccaacggc gaaacagttc
     5461 cgtatctcgt ctaactttaa cccgcgtcgt actaatccgg tgaccggtcg cgttgcacca
     5521 cacagaggtg ttgatttcgc catgccgcag ggtacgccag tgctttcagt gggtgacggt
     5581 gaagtggtgg ttgccaaacg cagtggcgca gcaggttatt atgtggctat tcgtcatggt
     5641 cgcagctaca ccacgcgtta tatgcacttg cgcaagattc tggtgaaacc gggacagaag
     5701 gtgaaacgtg gcgaccgtat cgcgctttcc ggtaataccg gacgttcaac cgggccgcat
     5761 ctgcactatg aagtatggat aaaccagcag gccgtaaacc cgctgacggc aaaactgccg
     5821 cgtaccgaag ggctgaccgg ctccgatcgt cgcgaattcc tggcgcaggc caaagagatt
     5881 gtgccgcagc tacggtttga ttaattaaca tccattcgca gccggtacgc agtcagtacc
     5941 ggcttttttt atttggtgcg gggcaagttg cgccgctaca ctatcaccag attgattttt
     6001 gccttatccg aaactggaaa agcatggaaa cgaaaaaaaa taatagcgaa tacattcctg
     6061 agtttgataa atcctttcgc cacccgcgct actggggagc atggctgggc gtagcagcga
     6121 tggtgggtat cgctttaacg ccgccaaagt tccgtgatcc cattctggca cggctgggac
     6181 gttttgccgg acgactggga aaaagctcac gccgtcgtgc gttaatcaat ctgtcgctct
     6241 gctttccaga acgtagtgaa gctgaacgcg aagcgattgt tgatgagatg tttgccaccg
     6301 cgccgcaagc gatggcaatg atggctgagt tggcaatacg cggaccggag aaaattcagc
     6361 cgcgcgttga ctggcaaggg ctgcagatca tcgaagagat gaggcgtaat aacgagaaag
     6421 ttatctttct ggtgccgcac ggttgggccg tcgatattcc tgccatgctg atggcctcgc
     6481 aagggcagaa aatggcagcg atgttccata atcagggcaa cccggttttt gattatgtct
     6541 ggaacacggt gcgtcgtcgc tttggcggtc gtctgcatgc gagaaatgac ggtattaaac
     6601 cattcatcca gtcggtacgt caggggtact ggggatatta tttacctgat caggatcatg
     6661 gcccagagca cagcgaattt gtggatttct ttgccaccta taaagcgacg ttgcccgcga
     6721 ttggtcgttt gatgaaagtg tgccgtgcgc gcgttgtacc gctgtttccg atttatgatg
     6781 gcaagacgca tcgtctgacg attcaggtgc gcccaccgat ggatgatctg ttagaggcgg
     6841 atgatcatac gattgcgcgg cggatgaatg aagaagtcga gatttttgtt ggtccgcgac
     6901 cagaacaata cacctggata ctaaaattgc tgaaaactcg cagaccgggc gaaatccaac
     6961 cgtataagcg taaagatctt tatcctatca aataaaaaaa agcctctcaa attattgaga
     7021 ggctgtactc gataaaaaat ctcaatgagt gttcccacta ttttgagttt ttaggttagt
     7081 acgctattga gttaatcgta gcgtgataat ttcaaaaggt ttgaactcaa gattgcaata
     7141 ttgattgcaa taaaaactgt catctaactt attttcttga agatcgacat attgaacgta
     7201 gtgataatta catacccacg aaagcatggc tgaaatttct aatccatgac tctcatataa
     7261 tcttacgatt ataccgtcgc cgtcttcagc taattttatt gtttcgatga taacgctgtt
     7321 agtatcgata gcaaaaaatg atagtgaagg gggtaattca cccgggtgcg gtttgacagg
     7381 gacgacctgt aagggatgat tcattatata ggcttgatta ataacaccac ccacacggta
     7441 atctccatga tgaggaaata aggagaatat gaatttttgt ttaccaatat ctgcaaaatt
     7501 attaatcagg aaattatcat aattgtctgt cttaacaact tccccaggtt tatgctgcga
     7561 acgcagcaaa gttaatttaa gcgtctggtc tttgacacga tagccatatt tgttttttgc
     7621 tattaaagct atgccagttt ctcgatcaga aaggtcgacc cagtgatgtg ctggtatttc
     7681 atctttggca aaagcgaacg aatcatcgct atgcgttggt ctgtcaatta caccgaactg
     7741 aatttgacat tgtgcaagag atgtttgtac tgacaccggg aatttgactt tgagagaaac
     7801 attaggttcg tgccaatcaa taaaagtttg gacatcaacg agtggactgt cctgtgtcag
     7861 ggtaaatgcg aagcgaagat aagaggaatt gtagtgataa atcagcgtca tcgtagtttt
     7921 aggcccgctg attacagttg tcattttttc agcgagtaaa acatatgatt ccccatcacg
     7981 ataatttgaa gcaaaatccc aggccgcgaa aaaacctgca tcttcgtggt atgcacggat
     8041 ctcactgtgc atattttcag taatgtattc tttaccatat cttttatcat atagcgaaat
     8101 taaatcaccg ttttctgaaa aaagaagttt aatgtaatta ttttcaatac ttcgagattc
     8161 tgccttcaga ccatttacga tcttattctt tggatcaatc acagcataac cataagagtt
     8221 tactcgtgct ttgagccagt tgttgtttat attgatccat tcgttacgag caaaacaggt
     8281 ggtattaaat aaaatgtgcg gatctttata agaagaagta tcaattttac ttaataaagt
     8341 agactgcgta tcagaaataa ttttctcggt ttttgcttct agtttaatat attccttttc
     8401 agtttcaaga taaactcttg aaatacatga tccgggaagg atgtcatgga attgcaaggt
     8461 cagcatctct ttccatattt catcaaactc actgcgaaga attgatgtca tattattagt
     8521 aatagttgta aaaaattcag catcatgtaa tttattttcc atatttcgat tatgagcttt
     8581 agttgctgac tctgatgtaa agcacccttg atgagcctcg aaataaagtt cacctgaaat
     8641 aatgggcaag ctttcgcgaa aagcgtcggc gtaagtgaag aacttatcaa cacggctgaa
     8701 atcaacatga ggtaagccat caa
//
LOCUS       DAJTMC010000167         8722 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.167, whole genome shotgun sequence.
ACCESSION   DAJTMC010000167 DAJTMC010000000
VERSION     DAJTMC010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8722
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.167"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            <1..189
                     /locus_tag="OO130_003490"
     CDS             <1..189
                     /locus_tag="OO130_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5366761.1"
                     /translation="ISSLVNSLKGVSGRLLRRDRPDIAVRYYYKGVLWSPGYFANSCG
                     GAPISVIRQYIEQQQTPG"
     gene            complement(320..1643)
                     /locus_tag="OO130_003491"
                     /pseudo
     CDS             complement(320..1643)
                     /locus_tag="OO130_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
     gene            complement(1643..3259)
                     /locus_tag="OO130_003492"
     CDS             complement(1643..3259)
                     /locus_tag="OO130_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR5366762.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMIWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGGWV
                     YTFLERILIPTGLHHFIYGQFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            3555..4271
                     /locus_tag="OO130_003493"
     CDS             3555..4271
                     /locus_tag="OO130_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR5366763.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLTPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEYQVEYHLRRLHPEKS"
     gene            complement(4268..5929)
                     /locus_tag="OO130_003494"
     CDS             complement(4268..5929)
                     /locus_tag="OO130_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HCR5366764.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAPALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(6125..6553)
                     /gene="ibpB"
                     /locus_tag="OO130_003495"
     CDS             complement(6125..6553)
                     /gene="ibpB"
                     /locus_tag="OO130_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HCR5366765.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(6665..7078)
                     /gene="ibpA"
                     /locus_tag="OO130_003496"
     CDS             complement(6665..7078)
                     /gene="ibpA"
                     /locus_tag="OO130_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HCR5366766.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(7072..7144)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            7384..7716
                     /locus_tag="OO130_003497"
     CDS             7384..7716
                     /locus_tag="OO130_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HCR5366767.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSHVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(7718..>8722)
                     /locus_tag="OO130_003498"
     CDS             complement(7718..>8722)
                     /locus_tag="OO130_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HCR5366768.1"
                     /translation="PKSEKYVFIHGPENPDETWYYDFHHRRGVIAEGGKVSNLDAMDI
                     TAPYTPGALRGGSHVHVFSPNGERVSFTYNDHVMHELDPALDLRNVGVAAPFGPVNVQ
                     KQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHALAFIGDTLSPKGEKV
                     PELFIVELPQDEAGWKAAGDAPLSGTETTLPAPPRGVVQRRLTFTHHRAYPGLVNVPR
                     HWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQSAFNWHPSGEWL
                     GFVLDNRIACAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLAWMEGGQLWITETDR
                     "
BASE COUNT         2217 a         2308 c         2191 g         2006 t
ORIGIN      
        1 atatccagtc tggtaaacag cctcaaaggc gtatcgggta ggttactgcg acgagatcga
       61 ccagatattg cagtcaggta ttactacaaa ggcgttttgt ggagtcctgg ctattttgcc
      121 aatagctgcg gaggtgcgcc aatatccgtc atccgccaat acattgaaca acagcaaaca
      181 cctggttagg tggaaaaccg cgccttatat ccccgccctg aaggacgggg ttttacggcg
      241 caccggataa atttggttat gcgccgtcag ccgtgctaaa gaatacggac cagcacccaa
      301 cggatgccag tccacacaat taacgtaact ctggccagta cgctttgttg gcctccagca
      361 actcatccag aatagctttg gcgaccgagg cgctcggtac cgttttcgat aacgtaatcg
      421 cctgccacag gtgctgatat gaacgctgtt cccaggcgtc cacgaccagt ttttccaccg
      481 ccacttgctg actcattaac cctttctgaa actgcgggat atcgccgacc accagtggtt
      541 ctggtccgtt gtgcccgacc agacacggga tctcgaccat cgcttcatca tcaaagttat
      601 ggattgcccc gttgttaggc acaatcagca acatccgctc ctgagtgttg aaggcaattg
      661 ccgccgccag atcgacgatg tatgacgcat gttcgtcaat ctccagcttg gccgccgctg
      721 aatttcctgc cgctgtaatg gcgcggcaag catcgaaaac ctgtttctcg cgatgttcca
      781 ttcacctcat tcgcgcgggt atgttcaggg ttggaatgct gaacaacata atccgggaag
      841 agataatatt tcagataggt gttcggcaat gtatccgggt ccagtgcctg gacatcccgc
      901 gcttttgcat aggtgtcgtt ccagctcgct tcagtatgtg tatcttgctg cggtggaaca
      961 taaccgtgct tagagacata ttgccgcagc tggggcatca ggtcgttgcc ctgcaaatct
     1021 tcaattgatg tccaccagcc aaagtggttc aggccgtagt agcgcacgcg catctgtttg
     1081 cgatcttgca gcccaacaat ttgcgccatc cggctttcaa taccgattgg catgtcacag
     1141 atgttgagga ttttcgcatt cgggcgcaga cgacgcgtgg cttctgcgac aattgctgcc
     1201 gggttggagt agttgagcat ccaggcattt ggtgaatatt tttccatata atccaccagt
     1261 tccaggacgc cgccaatgga acgcatgccg tacgctattc cgccgggtcc gcaagtttcc
     1321 tgaccaacaa cgccgtggcg cagcgggatt ttttcatcca gttcgcgcat cgggtatttg
     1381 ccgacgcgga tgtgcgccat gacaaaatca acgtcgctga atgccacttc aggatcggtg
     1441 gtgtaactaa acgcaatgtc cggcgctttc tctttaagga tgactttaca ggcttcggca
     1501 atcacttcct gccgcgcacc atcgttatca taaaatttca gtgcacgaag cgggaaacgg
     1561 tcctgattcg ccaggagcat caacacgatg cctggcgtaa aggtgcttcc accgcctgcg
     1621 acaaccactg agaatttggt cattatactg cctccgtaat ggcaacattt tctgctgatt
     1681 gatgagaatt aattaagcta tcgagctgtt cacgcagctg ggatacatgc agaccaatga
     1741 tcacctgaat ggcatcgcca ctacggaaga cgccgtgcgc tcccagcttt ttaaagactt
     1801 cgtcatccag cgtttgtgac atgtcatgca gtgcaatacg taaacgcgtc gcgcaattgt
     1861 taatgctgga gatattgccg accccgccca gggcttgcag gataccggca gcctgatcca
     1921 gctctttttt tggctctgca gcggtggttt ggcctcgcga ggctttgtat tcggcttttg
     1981 agtagagttt cacttccgca tcttcacgtc ccggcgtgca catgttgaat tgcagaatca
     2041 gtgtgcggaa aaccacgaag tacagcaggg taaagcacaa cccaatggcg atttgggtca
     2101 gcatcatatc cgcgtggttg ctgaacatcg ggatccagtt ttgcggtaaa acctggtcaa
     2161 tcagacctcc gcccatgttg cccaccacac caaagagata catcacggtc gacattgagg
     2221 ccgccagcac ggcgtgtacc gcaaacagca acggtgaaat gaacaggaag gtaaattcca
     2281 gcggttcggt aattcccacc agcatggcgg ttaaggttgc gggaatcagc aagcccgcca
     2341 cttttacccg attttccggt gcggcagtga agtacatcgc taaagaaatg cccacggcac
     2401 caaagatttt tgagttaccg tgcagggcaa aaccgccttc cgggaacaac gatttcagcg
     2461 gctcggcgct cagactgaac tcttgcagat gctgcgccca gtacatctga atgccgcctt
     2521 caacagctgc cggaccaaag ataaactgtc cgtagatgaa gtggtgtaaa ccggttggga
     2581 tcagaatacg ttcgaggaag gtgtaaaccc accccccaag cgcacccgcc gaacgcagga
     2641 acgcttgcag agattcaatc cccatttgta cttttggcca accgagcaac gtcagccagg
     2701 cacaggggat catcaccagg aaggcgataa tcaccacata agacgtcccc tggaaaatgc
     2761 cgagaaaaac cggcagtttt ttatcgaaca gacggttatg cagcgccgtc acaatgccgg
     2821 aaatgataat tgcgccgata atgctggtat cgagggtttt aatcccggcc atcattgtca
     2881 gaccgctacc tgccaccgcg tcctgagtga aatcgacgcc gaagtagctt ccccagatca
     2941 ttcccatcgc gttgatgaaa tagttccagg tcaggaaact caccatcacc gccagacaag
     3001 cacgcccctg cgcttgctta gcaaggccaa tgggtaaacc gacagcaaaa atcagcggca
     3061 tattacggaa taccgtccaa ccgccctctt caataatgtg tacgatttgc gcgaataaac
     3121 tgttcggatc ggtcagtgat tccccgacaa acatcgggtt ttgcagcaag atggcaagac
     3181 ccaccacaat cccggcgaag ggaaacagca gcactggcgt gaacatcgcg ccgccaaagc
     3241 gttgaatttg actgagcatt tccacattct cttttctcaa ttctgtaagt aggaagcctg
     3301 attggttatg cggcaggcct ggagcgagga taggaagatg cgatcggcag aacatgacga
     3361 cccaatggga ttcgtgatcg ctttcatgct tttcgttggg cggtagagat ctacgttttc
     3421 gcgaaaaagt agacatctgt gagcactggt acacagcatc gaacacatct ttaaaaaaaa
     3481 gatgtttttt caatcgatta agcagaactt gtgggcgcat tacccgggct tgcaggcaaa
     3541 aaagagatct agagatgatc tacaaaagca ttgcggagcg gttaagaatt cgacttaatt
     3601 ccgcggattt cacgctaaac agccttcttc ccggtgaaaa aaagttggcg gaagaatttg
     3661 cagtatcccg gatgaccatc cgtaaagcga ttgacctgct ggtagcgtgg gggctggtgg
     3721 tccgccgcca cggcagcggc acttacctgg tgcgcaaaga tgtgctgcat caaaccgcca
     3781 gcctgaccgg actggtggag gtgttaaaac ggcagggaaa aacggtcacc agccaggtgc
     3841 tgatttttga aatcatgcct gcgcctccgg ccattgccag ccagttacgg attcaaatca
     3901 acgagcagat ctacttctcc cgtcgcgttc gttttgtgga agggaaaccg ctgatgctgg
     3961 aagacagcta tatgccggtt aaactgttcc gtaatctttc gctgcaacat ctggaagggt
     4021 cgaagtttga atatattgaa caagagtgtg ggattttgat tggcggtaat tatgaaagcc
     4081 tgacgccggt gctcgccgat agactgctgg cgcggcaaat gaaggtagcg gaacacacgc
     4141 cactgctgcg gatcacctcg ttgtcatata gcgagagcgg ggagtttttg aattattcag
     4201 tgatgttcag aaatgccagc gaataccagg tggagtacca tttacggcga ctccacccgg
     4261 aaaagagtta accgatactc cagaagagca ccgccagtaa ttggggggtg ataattcgca
     4321 gaaacatcac taacggatag acagtggcgt aagagagcgc cggcgcacca ctggttggat
     4381 gaagattatt agcaaacgcc agcgccggag gatcggtcat ggaacctgcc agcatcccgc
     4441 acatggtcag gtaattcatt ttggctaaca tccgcgccag aatgccaaca gtaatcagcg
     4501 gaacggcggt gatcagggca ccataaccaa tccagcttag cccttcgcca ttgaccaggg
     4561 tattcacaaa atccccacca gatttcagac caacgaccga gaggaacagc acgatcccca
     4621 gctcccgcag cgcgaggttg gcacttggcg gcataaacca gtacagcttg ccgatactgc
     4681 cgatacgccc gaggatcaac gccataatca gcggaccgcc cgccagcccc agtttcaacg
     4741 cggccgggaa cccaggcaca aagacgggaa tagaacctaa caatacgcca agcccgatgc
     4801 caataaacac tggcagcatc tgaacctgtt gcagtttttg ctgcgcattc cccagcacat
     4861 tggcaacggc atcaattgcg gacggacgcc ccaccagatt gaggatatcg ccgaactgca
     4921 ggctgatatc gccactggcg accagttcga ccccggcacg gttcaggcgc gagataacaa
     4981 cgtcatagcg ttctttaaag tgcaggtcgc gaatacgttt tccgagcacg ttttcattgg
     5041 tgaccaccac acgctcgacg cgcaaatcag tgcctttcgt ggacagcgaa gtatcgacct
     5101 cctgaccaat caccagttgc gcattatgta aatccgctgg ctgccccacc agatgcagca
     5161 aatcgcccag ttggataatg gtatctggcg aaggaacttt tagggtttct tcgcgtttca
     5221 gacgcgagca gataattttg tcgccgttga gaatcggtac atctttaata gctaaatcat
     5281 gcaggttagg gttctcaacg cgaatattga tagtcttgat cagcgcgccg ccattggtgc
     5341 gtgaagactc gtgctgctga gcttctgtct cgacattgac gcggaaaata acccgcaaca
     5401 tccacatggt gaacaaaatc ccgcaaatgc cgaatggata cgccatcgcg taactcatcc
     5461 ccatctgatc gaccatttcc attggtgtac ccaggtcgcg caagatctgc tgccctgccc
     5521 ccagcgctgg cgtattggta accgcaccgg agaaaatccc cagcactacc ggcagtggaa
     5581 tatcaaacag tttatgcagg atggcggtaa ccagaccacc gatgatgacg atcagaacag
     5641 caaacaggtt gaggcgtaat ccggagacgc gcaatgaggc aaagaagccc ggccctactt
     5701 gaatcccgat ggtataaacg aacaggatca ggccaaattc ctgaataaca tgcagcatat
     5761 cgctacttaa tgtcatcccc gcctgagaaa caaaatggcc gacgatgatc ccaccaaaca
     5821 gcacgccgcc aatacctaat cctatgccgc gaaatttgac gttgccgata aacaaaccga
     5881 cgactgccac caaagccaga atactgaccg ttaatgctat atcactcatc gtccatttct
     5941 cttgagataa cattttaatt gctacggatt atggctgaat cggtgaagca gatttgcctt
     6001 atagcgcaca ttatcacggg aaagaacgtg ccgaaatatc ttaaacagtc gaaatttaac
     6061 gcattgttga agaagaaaga caaaaaaagc cccaccagaa tggcggggca aagagaatag
     6121 ctagttagct atttaacgcg ggacgttcgc tgatagcgat acgctgcgct gcgatgggtt
     6181 caggctcatt acgaattaaa tcaatatgca gtaaaccgtt tacgaaggtt gcgccagaga
     6241 cttccatatt ttcagccagc gtaaagctca ggctaaatgg ctgattcata agcccttgat
     6301 gcagccattt tttctcttct tttggctgct ccggcgtgcc ttttacgctc aggcgcgtac
     6361 cttccagttg aatctctaaa tcttcctgac ggaaacctgc cagcgcaagg gtaatgcggt
     6421 agtggttatc gtcgcttttc tcaatgttgt acggcgggaa gctctggctt tcaccggcgt
     6481 tttgcagtgc gttggccagt ttgtcaaaac cgatccattg acgcatcagt ggggataaat
     6541 cgaagttacg catagtcatt tctccttcta agaagcgagt aagtacctgc aaatccgaag
     6601 attcgcatat gctccctgac ggcgagcatg gagatgtcag gccgcgtcag gcggccttag
     6661 ggaattagtt gatttcgata cggcgcggtt ttttcgcttc cggaatcacg cgttcgagat
     6721 cgatatacag caaaccattt accaggttag ccccacgaac atgaatgttc tcagctaact
     6781 ggaatttgcg ttcaaagttg cgttcagcga tgccctggta cagataggtg cgctcttttt
     6841 gttcgtcggc gtgagcacct ttcaccacca gcagattatc ctgggcggta atttccagtt
     6901 cgctctcagc aaaaccagcc acagcgatag caatgcggta atggttttcg tctaccagtt
     6961 caacgttata cggagggtag ccgccattac tctggctctg gttgttttct aagtggttaa
     7021 acaaacggtc aaatccaata gcagaacggt aaagcgggga taaatcaaag ttacgcataa
     7081 tcaatagctc ctgaaatcag cgagaatgta ataccttcca caatggacag gtcaggtagc
     7141 cagaacaccc atcaggcgcg tcctcatcgg ctacgatgta aaaatgggtc tggaaatgaa
     7201 cttttcaagc cttatcagcg gacttttttt tgcagtttat ggtctattgc atagactgag
     7261 ggggcagcac gaaacgttaa agtgcgatag ccgccacagt gcgacgtaat gatgatgtta
     7321 ttttcagcaa gggatcgcta taactcatca ggccaacacc cacgatcaac agatgaattt
     7381 attatgataa gaaatgtgtt gttagcgttc atgatatgca gcggaatgac attactcgga
     7441 ggatgctcca gcgtgatgtc ccataccgga gggaaagaag gaacgtatcc cggcacccgc
     7501 gccagcgcaa ccatgattgg cgacgatgaa accaactggg gcactaagtc gctggcgatt
     7561 ctcgatatgc cgtttaccgc tgtgatggac acgcttttac tgccgtggga tgtgttccgc
     7621 aaagacagct cggtgcgctc acacgttgaa aaaagcgaag ccaacgcgca agccaccaat
     7681 gcggttattc caccggccag aatgcctgat aactgattta gcgatcagtt tcggtgatcc
     7741 acagctggcc gccttccatc cacgccagcc attgaccatc cggcgagaag accacggcgt
     7801 ccgcagaagg tggattggcg tggttttcgg ttaaatactc aacctcgcca ctttgcgcat
     7861 gggcacaagc aattcgatta tccagcacaa agcccaacca ttctcctgac ggatgccagt
     7921 taaatgcaga ctgaatatcc gttttgttat gggttaactg gcgcggctcg ccgccctgtg
     7981 gcgagataag ccacagttgc acaatgccgt tatcatcacg cattaaaaac gcgatttgcg
     8041 taccctgcgg attacagcgc acccagtggc gcgggacgtt gactaacccc ggataagccc
     8101 gatggtgggt aaaggttaaa cgtcgctgca cgacgccgcg cggtggcgcg ggcagggtgg
     8161 tttccgttcc acttaacggc gcatcacctg ccgctttcca gccagcttca tcttgcggta
     8221 actcaacgat aaacagctcc ggcactttct cgccctttgg cgaaagtgtg tcgccaataa
     8281 acgccagcgc gtgatttcct acccatcctt cttcataagc acgattgatt tcatcgctgc
     8341 caggctgcgg cgtgggcgtg gttttactca ccagcacgca ccagtggcta ccgctgtatt
     8401 cacgcggatg ctgcttttgt acgttgaccg ggccaaacgg cgcagcaaca ccgacgtttc
     8461 gcaaatccag cgccggatcg agttcatgca ttacatggtc gttataggta aagctcaccc
     8521 tttcaccgtt cgggctaaag acatgcacat ggctgccgcc gcgcagcgct cctggcgtat
     8581 acggcgcggt aatatccatt gcatcgagat tgctcacctt gccgccttca gcaatgactc
     8641 cgcgtcgatg atggaaatcg taataccatg tttcatcagg attttccggg ccatggatga
     8701 aaacatattt ctctgacttc gg
//
LOCUS       DAJTMC010000168         8698 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.168, whole genome shotgun sequence.
ACCESSION   DAJTMC010000168 DAJTMC010000000
VERSION     DAJTMC010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8698
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.168"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            107..283
                     /locus_tag="OO130_003499"
     CDS             107..283
                     /locus_tag="OO130_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HCR5366769.1"
                     /translation="MKRLNKLRGWRKVSRSAILREAVEQYLERQQFPVRKAKGGRQKG
                     EVVGVDDQCKEHKE"
     gene            502..744
                     /locus_tag="OO130_003500"
     CDS             502..744
                     /locus_tag="OO130_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5366770.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(1074..2003)
                     /gene="fdhE"
                     /locus_tag="OO130_003501"
     CDS             complement(1074..2003)
                     /gene="fdhE"
                     /locus_tag="OO130_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HCR5366771.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLDDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(2000..2635)
                     /gene="fdoI"
                     /locus_tag="OO130_003502"
     CDS             complement(2000..2635)
                     /gene="fdoI"
                     /locus_tag="OO130_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCR5366772.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(2632..3534)
                     /gene="fdoH"
                     /locus_tag="OO130_003503"
     CDS             complement(2632..3534)
                     /gene="fdoH"
                     /locus_tag="OO130_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HCR5366773.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(3547..6597)
                     /gene="fdnG"
                     /locus_tag="OO130_003504"
     CDS             complement(3547..6597)
                     /gene="fdnG"
                     /locus_tag="OO130_003504"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(6010..6012),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCR5366774.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVANYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            6791..7624
                     /gene="fdhD"
                     /locus_tag="OO130_003505"
     CDS             6791..7624
                     /gene="fdhD"
                     /locus_tag="OO130_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HCR5366775.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
     gene            7778..>8698
                     /locus_tag="OO130_003506"
     CDS             7778..>8698
                     /locus_tag="OO130_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiG family protein"
                     /protein_id="HCR5366776.1"
                     /translation="MKRNLLSSAIIVAIMALSLTGCDDKKAETETPPPANSQPAAPAP
                     EAPVAKAEAKPETPAQPVVDEQAVFDEKMDVYIKCYNKLQIPVQRSLARYVDWLKDFK
                     QGPTGEERTVYGIYGISESNLAECEKGVKSAVALTPALQPIDGVAVSYIDAAVALGNT
                     INEMDKYYTQENYKDDAFAKGKTLHQTFLKNLEAFEPVAESYHAAIQEINDKRQLAEL
                     KNIEEREGKIFHYYSLAVMISAKQINNLISQEKFDVDAAMKKVSELETLVAQAKEADK
                     GGMNFSFINSAGQYQLEAKKYVRRIRDKVPY"
BASE COUNT         2072 a         2197 c         2291 g         2138 t
ORIGIN      
        1 ctctttagag taacgttggt tttttctgct cattattagc tccttctgat gccattctat
       61 ttcaggaagg agtgtccgtt aaactcaggc tacctcagaa gaggcaatta aacggctgaa
      121 caagcttcgg ggctggcgta aggtttcacg atctgcaatt ttacgcgaag cggttgagca
      181 atatctggag cggcagcaat ttccggtgcg taaagcaaag ggaggcaggc aaaaggggga
      241 ggtagttggt gttgatgatc agtgtaagga gcataaggaa tgaaaaaaat ttttcattct
      301 tgaatataaa aaaacagatg cccttattct ggtattaata caaggctgtt ttacttgaac
      361 ttataataac tgcaactgtt acatcgtatc tggaaaacgc ctcgcaaaaa taaaaaatga
      421 tgcgtaaatg agctttcttt aaagattcgg caagggtact tttctctcca tagattcaaa
      481 aatgatattg gcgagatatt tatgaactct ctggcaggta tagatatggg cagaatttta
      541 ctcgatttat cgaatgaggt gattaagcaa cttgatgatc tcgaggtgca gcgtaatctt
      601 cctcgtgcgg acctattaag ggaagcggta gatcaatacc tgataaatca atcgcaaaca
      661 gcaagaacca gtgttcctgg catctggcaa gggtgtgagg aagatggtgt cgaatatcag
      721 cgtaagctgc gcgaggaatg gtaatgggaa aaaatgatgt caatcagatt gctgacaacg
      781 tgcgcgttgt tcatgccgga tgcggcgtga acgccttatc cggcctacaa agtcatgcaa
      841 attcaatata ttgaagttta ttggtaggcc tgataagcgc agcgcatcag gcaatcatac
      901 gcttgtcatc agtgacatac ccataacctt atcgatgtgt tggagggaag aacgaggcaa
      961 acagacaatt gcggcatgat cttgaatggg taacgcggaa tacgagagat ggtgtcgtca
     1021 caccctatga gctgtaatcc gggcgaccag cgccacccgg aaaacgtcag agattactcc
     1081 ccttcacccg gaaacaggaa cgggttgatg gaactgcggg catagccttc ttgctccatt
     1141 cgcgcgtcca gtaccagaga agcgaggtca tctgccacgg cttcaacttt cggttctttt
     1201 tcctgataaa gaatcttcag gtaagtgccg cagtcatcgc agctttcggc tttaatcgcg
     1261 gcctgttcgt catccagcga ccagtaatgc agtttgccgc tctgttcaca gttgctgcat
     1321 tttacgcgca ctacgtgcca ttcggtttca cacaggttgc agtgcaggta acgcagaccc
     1381 tgagtggtgc caatttgcac catgctggac accggcatag aaccacaaac cgggcaatat
     1441 tgacgttgct cgccgtattc agcgcgggct ttgccgggga tcagattggc catctgcgcc
     1501 cagtagagcg acagtgcagc ccagataaac ggcgctttat cgctgctgac ggatgagaaa
     1561 tcagaggcaa acagtgcgct ggccatatct tccagctcct gagtcgatgc cttctccaga
     1621 ttctcaatca ctgccagcgc cgggccgctc atttcaggtt tcagctcagc aatcagcgcc
     1681 atcagcagct tttgccagtg cttatcacgc ggcagaacgt gaatatccag cgggggcttg
     1741 ccctgtgcgc tggcttcttt aatgcgcgcg gtcagatcca tctccagcgg atggtcgtac
     1801 agcaccactt cctgggcgtg ggcgataagc gcagcaaagc gcaggtaatc atccagcgga
     1861 ttattttctg ccagctcgcg cagacgctcg gcgcggcggt tgtataaatt cttgagccga
     1921 gggaacaata acggcggaat catatccgcc gtacgtttct cgctcgaacc cagctcatct
     1981 tgcgggatta tgcgaatact cattcagctt tcttttccgt tgtcttgcgg acctcacggt
     2041 accagcgcgg gtgatgtttc ttcgcccagg cgctggtaac ccatccttcc accatggcgg
     2101 taatcgtgcc tttcacccaa agggcggcgt agatatgcac catgataacc acaattaacg
     2161 ccactgcggc aaatgaatgc agcattaacg cgaatcggat caccgggatt gagaaagcag
     2221 gcgcaaaata aggacgccag ataatcacgc cgctcaccag cagcagaacc aggaaaataa
     2281 tcgccgccca gaaaacgcat ttctgaccga agttataacg cccggtgtca cctacttcct
     2341 cgttgacgac gatcttacga atattcttcg cccaaaagat atcatcccga ttgattaggt
     2401 tgtgatgcca gtaacggaaa aacatgatga tgaacgaggc aaacataacc acgccgacaa
     2461 acgggtgcag aattcgcgcc agctgcggtg tgcccatgat ttgcatcaac cagttgaagg
     2521 acgggaacaa aaagcccagc ccgctcaccg ccgctaggat gaagcagaag gcggtgatcc
     2581 agtggttgat acgttccggc gcggtgtagc gcacgatggt gtcacgtcgt ttcatttgcg
     2641 ctcctcgtct ttctcttcgt gcagattatt ctcttcctca tccgcacggt tcggaccgac
     2701 acccacgtag tggaagatac tggctgcgaa ggtagccgca aagccaacag ctgcgagcgg
     2761 tttccagatg cctttccaga atttcacggt ttcgctgatt tccgggttct ccggcaagcc
     2821 atgatacaga tttggcttgt cagcatggtg cagcacgtac ataacgtgtg taccaccgac
     2881 gcctgccgga tcgtacaggc ccgcattgtc gtaaccgcgg gttttcagct ccgccacgcg
     2941 ctcgctcgcc agcgttttca tcgactcttt cgtaccgaag tgaatcgcgc ccgttgggca
     3001 ggtcttcacg caggctggtt cttgcccaac caccacgcgg tcaacgcaca gcgtacattt
     3061 gtagacgcgg ttgtcttccg ggttgaggcg cggaatgtcg aacggacagc ccgcaatgca
     3121 ataaccgcag ccaatgcact gctcggactg gaagtcgacg ataccgttgg catactgaat
     3181 aattgcacct tccgccgggc acgctttcag acagcctgga tcggaacagt gcatacagcc
     3241 gtctttgcgg atcagccatt ccagtttgtc gttctgctcc acttccgaga agcgcatcac
     3301 cgtccacgat ttggcactta aatcattggg gttgtcgtac accccaatgt tattgccgac
     3361 ggtatcgcga atgtcgttcc actctgaaca cgccacctga caggctttac agccgataca
     3421 ggtggtgacg tcgatgagtt tcgccacttc ttcctggaag tcccgcgcct gaggcgcggg
     3481 ggtcagaccg ttagtcgcgg aacgacgaat gatatcttga gattgataag ccatatgtcg
     3541 tctccgttac accttttcca cattcacaag gaaggactta aactccggcg tctgcgtgtt
     3601 cgcatcacca acgaatggcg ttaaagtatt ggcaataaag ccttttttag ctacgccttc
     3661 atagccccag tgaatcggaa taccgatggt atcgatatct ttgccgttcg ctttcagcgt
     3721 gcgaatacgt ttggtcacca ccgctttggc tttgatatag ccacggttgg aggagacttt
     3781 cacggtatcg ccctgggcaa tgccaagttt attcgccagc gactccccga tttccacaaa
     3841 ctgctctggc tgcaaaattg cgttcaacag cgcgtgtttg gtccagtagt ggaagtgctc
     3901 ggtcagacga taggtggttc cgacatacgg gaacttatcg gctttaccca atgcttcggc
     3961 gtcgtcttta aagatacgtg cagccgggtt cgagataacg tttggatgca gcgggttagt
     4021 tcccagcggc gtttcaaacg gctcgtagtg ttccgggaac ggaccttccg ccatcttatc
     4081 gagggcaaac agacgcccca tgccttcctg ctgcatgata aacggcccga cgccgctacc
     4141 cggaggcgct gcgctgtagt ccggaatatc ccagccggtc cacttagtgc cgtcccattt
     4201 cagcaactga cgcttcggat cccacgggtt accctgcgga tctgcggagg cgcggttata
     4261 cagaatgcgg cggttaagcg gccatgccca tgcccagccc agcgtgttac cgaggccaga
     4321 cggatcggcg ttatcacgac gcgccatctg gttgccttcc ggcgtccagc taccggcgaa
     4381 aatccagcag ccacaggagg ttgtaccgtc atcgcgcaat tgggcgaacg agctaagttg
     4441 ttggcctttc ttgacgataa ccgcaccggt tgccggatcg gtaatatcgg ccagcgcctt
     4501 accgttgctc tccatcgcta cttcttccga cttcggctca tgcggaatgg cgtagttcca
     4561 ggtcatgttc agcacctgat ccgggttcgc gccaccctgt tcggcataca tcttgcgcaa
     4621 gcgcaggaag ataccggaga ggatctcacc gtcagtcagc gcaatccccg gggcgtccgc
     4681 acctttccag tgccactgca accagcgacc tgagttaacg attgaaccgt tctcttctgc
     4741 gaagcaggtc gatggcagac ggaacacttc ggtctggatc ttcgacgagt caacttcgtt
     4801 cagctcaccg tggttctgcc agaagttaga ggtttcagtg ttcagcgggt cgatggtgac
     4861 gaggaacttc agtttcgaca gacaaccgat cactttgttt ttgttcggga atgaggcaac
     4921 agggttaaaa ccctggcaga tatagccatt gaccttgccc tctttcatca tctcgaagta
     4981 ttgcaggacg tcgtagcctt tatcccactt cggcaaccag tcaaagcccc agctattttc
     5041 cgccgtcgct ttatcaccaa agaaggcctt catcatagag acgaagaatt tcgggtagtt
     5101 gccccagtag tttacctggc cttccagcag cggttttggc gtgttggcgg taaggtaggt
     5161 ttgcagatcg gtctgcttct cgcttggcag cgtcatgtaa cctggcaggc tctgcgacag
     5221 cagccccagg tccgtcagcc cctgaatatt ggagtgaccg cgcagggcgt taacgccgcc
     5281 gcctgccatc cccatattgc cgagcagcag ctggatcatc gccatcgtac gaatgttttg
     5341 cgcaccaacg gagtgttgcg tccagccgag ggcatacagg aacgaggcag ttttatcgtg
     5401 agcactggtt tctgcgatgt attcgcagac tttcaggaac gcgtcttttg gcgtaccaca
     5461 gatgttttca accacatctg gcgtgtagcg ggaaacgtgc tgtttcagca agttccacac
     5521 gcagcgcgga tgttgcagcg tggtatcgcg tttggcgaag ccgttttcgt ccagttcgta
     5581 agtccaggag gatttatcgt acttgcgttt ttccgcgtca tagccggtga aaaggccatc
     5641 ttcaaagccg taatcctcac gcacgatcag gctggcgttg gtataggctt cggtgtattc
     5701 gcggttgaat ttttcattgt tcagcaggta cagcaatacg cctgacagga aagcaatgtc
     5761 agtaccggaa cgaatagggg catagtagtt agctaccgcc gccgtacgcg taaagcgagg
     5821 atcgatcaca atcagcttcg cgccgttgtg aattttggct tccatcgccc agcggaaccc
     5881 gaccgggtga gcttcagcgg cgttaccgcc catcaccacg acgaggttgg cgttcttgat
     5941 gtcgacccag tggttggtca tcgcaccgcg accaaatgtt ggagcaagac ttgctaccgt
     6001 tggtccgtgt cagacacgcg cctggttgtc gaccgcgagc atacccagcg cgcgggagaa
     6061 tttttgcgtt aaatagccgg tttcgttact cgacgcggaa gcacacagca tcccggtgga
     6121 gagccagcgg ttaacagtca cgccttcggc gttttgcgca atgtagttag catcgcggtc
     6181 ttctttcatc agtttggcga tgcgatcaaa cgcctcttcc caactgattt gctgccattt
     6241 atcagaacct ggcgcacggt attccggaaa cttcagacgg ctttcggagt ggatgaaatc
     6301 caccaggcca gcgcctttcg gacaaagtgc accgcggttg accgggtgat ccggatcgcc
     6361 ttcgatatgg aagatagatg ctttggcgtt ttttgctccg tcaccgaggc tgtacatcaa
     6421 cagcccacag cctacggaac aataggtgca ggtattacgg gtttcgcggg tgcgcagcag
     6481 tttatactgc cgggtttccg cgagtgctac gctgggtgca aaacctagtg ccgctgccgt
     6541 ggtgcctgcc ataccgccag cgcagatctt aaagaactgc cttctgctga cctgcatgga
     6601 ttgctccttg tttcgacatt gtcacgtccc atttacattc gcttgctgcg tgtgcaggga
     6661 gtgggagtta tttttctttg cggaaggggc cgcaaaggtc cagaattggc tcgatttccc
     6721 tccatccagg aagggtttgt aacagaatac cataatgttg gtgtgtgtgt tcttatctgg
     6781 ttaagagaaa gtgaaaaaaa cacagcgaaa agaaatcgaa aatgtgacaa atatcacagg
     6841 tgttcgtcaa attgagttat ggcggcgtga tgatttacaa cacccacggt tagatgaggt
     6901 cgcggaagaa gttcccgttg cgctggtcta caacggcatt tcgcatgtgg tgatgatggc
     6961 gtcgcccaaa gaccttgagt actttgcgct cggtttttcg ctttccgagg ggattatcga
     7021 aagtccgcgc gatatcttcg gcatggatgt cgttccttcc tgtaatggtc ttgaagtaca
     7081 aattgagctt tccagccgcc gctttatggg gttgaaggag cgccgccggg cgctggcggg
     7141 acgtacgggc tgtggcgtat gcggcgtgga gcaacttaat gacatcggaa aaccggtgca
     7201 gccgctaccg ttcacccaga cgtttgatct caacaaactg gatgatgcat tacgtcatct
     7261 caacgatttt cagccagtgg ggcaactgac tggttgtact cacgccgctg cctggatgtt
     7321 gccatctggc gaactggtcg gcgggcatga agacgtgggt cgccatgtgg cgctggacaa
     7381 actgttaggc cgccggtcac aagaagggga aagctggcag caaggtgcgg tactggtttc
     7441 cagtcgtgcc agttatgaaa tggtgcaaaa gtcggcgatg tgcggcgtag agattttgtt
     7501 tgcggtgtct gccgcgacca cgcttgctgt agaagtggct gagcgctgta atctgacact
     7561 ggtaggtttt tgtaaaccgg gtcgggcaac ggtttatacc catccgcagc gtttgagcaa
     7621 ttaattaaaa taagtcctgc gatatatatt aaattgcagg acttattcat ttcgtgaatt
     7681 ttattatttt tatttataaa taaaacgcgt gtagtggcgt tttctttttt catttattat
     7741 gttcactctc ttatctatat atttataagg caattaaatg aaaaggaatt tattatcttc
     7801 cgctattata gtcgccatca tggccctcag tctgacgggt tgtgatgata aaaaagccga
     7861 aacagaaacg cccccgcctg ccaatagtca acctgccgca ccagctcctg aagctcccgt
     7921 tgcaaaagca gaagctaaac ctgaaacacc tgcgcaaccg gtggtcgatg aacaagcggt
     7981 tttcgacgaa aaaatggatg tctatatcaa gtgctataac aagttacaga tccctgtaca
     8041 gcgcagtctg gcgcgttatg ttgactggct gaaagatttt aaacaggggc ctaccggtga
     8101 agagcgtact gtttatggca tctacggcat tagtgaatcc aacctcgctg agtgtgaaaa
     8161 aggcgtaaaa agtgctgtgg cgttaacgcc tgcgctgcaa ccaattgatg gcgtggcggt
     8221 gagttatatc gatgctgccg tggcgttggg taataccatt aacgaaatgg ataaatatta
     8281 cacccaagaa aattataaag acgatgcgtt tgcgaaaggg aaaacgctgc accagacatt
     8341 cttaaaaaat ctggaagcct ttgaacctgt agcagaatct tatcatgcgg cgattcagga
     8401 aattaacgat aagcgccagc ttgcggaact gaaaaatatt gaagaaagag aagggaaaat
     8461 attccactac tattctctgg cagtcatgat ttcagcgaaa caaattaata acctgatatc
     8521 gcaagagaag tttgatgttg atgcggcaat gaagaaagtg tctgaactgg aaacgctggt
     8581 ggcgcaggcc aaagaagcgg ataaaggcgg catgaatttc tcgtttatta attcggcagg
     8641 ccagtatcaa ctcgaggcta aaaaatacgt tcgccgcatc agagataaag tcccgtac
//
LOCUS       DAJTMC010000169         8652 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.169, whole genome shotgun sequence.
ACCESSION   DAJTMC010000169 DAJTMC010000000
VERSION     DAJTMC010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8652
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.169"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(<1..271)
                     /locus_tag="OO130_003507"
     CDS             complement(<1..271)
                     /locus_tag="OO130_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HCR5366777.1"
                     /translation="MYRAAATHPKIKLLQFKLIPCPGMNKAIPSLIADFTGELHRLIS
                     EHLQCKRRKDKSVEPTPLYYLWSREAVANDATPYLMVLLLNNDVWC"
     gene            complement(365..927)
                     /locus_tag="OO130_003508"
                     /pseudo
     CDS             complement(365..927)
                     /locus_tag="OO130_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004714555.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
     gene            complement(1131..1337)
                     /locus_tag="OO130_003509"
     CDS             complement(1131..1337)
                     /locus_tag="OO130_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HCR5366778.1"
                     /translation="MVYKSDSDELAVLRAENTRLVSLLEAHGIEWRRKPQSPVQCVFV
                     LSTDEKVALFRRLFRGRDDVWALR"
     gene            complement(1444..2007)
                     /locus_tag="OO130_003510"
     CDS             complement(1444..2007)
                     /locus_tag="OO130_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HCR5366779.1"
                     /translation="MYNANPNYKMDFMILKDVNEHMDGMFQRFSKLLPFRIDFAYRKD
                     TPSFGHSCKHSMCMEIYRLLSETQTMLAGYYWVMEYTPDKGLHIHFIGYLDGQRHKNS
                     YQISRQLGDIWRRITEGEGYFHLCRAKDKYPVRIDHVIHYSDKSAVDDLRYALSYLAK
                     QDQKEHGIILGRSRLPEKSNRGRPRHN"
     gene            complement(3382..5217)
                     /locus_tag="OO130_003511"
     CDS             complement(3382..5217)
                     /locus_tag="OO130_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5366780.1"
                     /translation="MRTFTNMLYDICTVLGLFKEGENPAHKRKSTNFEMHQKFWDQRY
                     NEISRIIDAEGVFSQEQRRIIYARYEHFYYMMNSYPVHSTLKPEFLRSYCLRTFGVIF
                     LVVDMYNTYRPENDSAFYYHIYNFLQKSYCPCLDHADTESDEAAVKRYLREYLAELGF
                     NKEDFHENGKLYALGKYTGTIRKDNGKSKSLMQQYIMAIKNEYKKDYREKKLDKDELE
                     KVLRNIDKFYNAFYSLSVLLDIQRKTKILQSLAYYLRVLVREGLWIHGLYGYAAQYLY
                     DFTSFDTTPYAKKLLEMFYKFQNSAEGTLSRYSVSLDDKSQEYISRLKDLVFNINDKN
                     GCDDAYLKKIISYFGQLQNEAVHVTSCYETLAVYICLIRKNKINDVLQHYDDMERKGL
                     FGELPSGYVRGALSLLRTALEVKVNRKNIKYGSLFYWLDHVKAYQDAFIEKIPLIDPV
                     YKEGEIQYDANNFTLMRVIKMYNCMLEKISTKPYIAPPYITGLLDDVEKVLDKINILI
                     DKEYVYDGKTLAEVIMENKVLSSRERKETMIGLFTGSKKYTLLQCVEKLGVLVHYVKS
                     PVDEIKNVMMLYGDKAENRNRRRMIYDALTIICEDDIRNNPPELS"
     gene            5318..5605
                     /locus_tag="OO130_003512"
     CDS             5318..5605
                     /locus_tag="OO130_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000070930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCR5366781.1"
                     /translation="MSNQTGQLYTPSRILRMAELTRLLGISRSTIYVKINPASKYHDP
                     TFPKPIRLGSASVGWRASAIDEWLMLHTAPAWSEPENKLKELNDDDKTITP"
     gene            5577..7106
                     /locus_tag="OO130_003513"
     CDS             5577..7106
                     /locus_tag="OO130_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HCR5366782.1"
                     /translation="MMTTKLSLPELDTTKLPDRCQALLDEMHEETGIRREILLSVMLT
                     VMAASVQDTHEVELSGGQRTSLQIFMCLSSASGSGKTSACAKLIAPVHETEEELHQAY
                     IDDKKNYDRMMEMWTTDKKILERIYKKEIERSPENAAAARAALEKCIANKPEPPVQQV
                     LIVNDATPEGIALKLSQSPSLLLLSDEGGTILDKRFERKSALYNTLWSGQPVNVERAS
                     RPGFRLNDVRLTILILTQPVIFNKFFTLTGDQIRGNGFLARLLFCEPGAIKIMTTEPH
                     TATPVVTQQCASCFRKKSFGSQIRDSLRASRERRAKSKERICMTLSNAASRALERFHK
                     ENMDTVRQNPHMGTFEDIIVRKREQVVRIAALLELEKDPDSTVITLESTNSAIYLIEF
                     YFKHLIYKLESLREISPAEKLDKWLQKRIITTAGYIFQKSYILQYAPYALRKKCVLDE
                     ALDILAEQRKIRIDDNLVVYIGNTITPSELAKKLNIPAFDAGVFICDHQNILKYHRNR
                     L"
     gene            complement(7276..8493)
                     /locus_tag="OO130_003514"
     CDS             complement(7276..8493)
                     /locus_tag="OO130_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000279856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5366783.1"
                     /translation="MAVLTDTKARHIKPDDKPLPHGGITGLTLHPSSVKGRGKWVFRY
                     VSPVTQKRRNAGLGTYPEVSIAEAARTARIMREQLAAGDDPLEIKKAESEKVVIPTFA
                     DAARRVHAELSPGWENPKHVRQWLSTLENYAFPQLGAKTLDSITAADVAETLRPVWLT
                     LSETASRVKQRIHVVMQWGWAHGFCVANPVDVVDHLLPQQTRGRDEHQPAMPWRQLPL
                     FVATSVYSDEPYNVTRALLLMVILTATRSGEARGMRWAEIDFHKRVWTIPAERMKARL
                     QHRVPLSRQAIYILENIRGLHDELVFPSPRKQQILSDMVLTSFLRKKKAVSDIPGRVA
                     TAHGFRSTFRDWCSEQGYSRDLAERALAHTLKNKVEAAYHRTDLLEQRVPMMQAWADY
                     VMSQLAKNTKEFA"
BASE COUNT         2682 a         1955 c         1616 g         2399 t
ORIGIN      
        1 tacaccagac atcgttatta agtagcagca ccataagata aggagtggca tcgttagcca
       61 cagcttccct actccagagg taatataaag gggtgggctc gactgattta tctttacgtc
      121 gcttacactg caaatgttca gaaattagtc tatgcagctc accagtaaaa tcagcaatca
      181 gagaaggaat tgccttattc ataccagggc aaggtattaa tttaaactgt aataatttaa
      241 ttttaggatg tgtagcagca gcccgataca gagttgcaag gacacacttt tgccagaggg
      301 cgttacagga aagcttaatg tttgattctg tatacataat aaatcacctt atagttacaa
      361 taggtcaaaa accgctgtag ccaaagttac gctggcctga tgctttagtg tcgagcttcg
      421 tcaaataatc cagacgtacc aataagcgct ggtactgctc tgggaaatta ggatcgttaa
      481 tatccaggat gtaacgtcca ttagcaggga aatgaactaa tccctggtga tcttccagtt
      541 ccaaccctaa ggcactgtac caggctccgg taattaaccc tctcagggtg tcagagcgct
      601 catagtttcc cagatggtaa taggcgtctt tgttaaaaaa aggcagacgt gataatgaca
      661 tttaaaggac tgcgtatatt ctttcgccca gataatacaa accgacgttg cgtaggtgcg
      721 tttgccttgt tgttttttac ggtgttgatc tgcattaatt ttcgcattca gcgagttacg
      781 gaaacgagat atctctccgg ggatcaggtt agggaaacag gtaatgatat ccccgttgtc
      841 aatcacagga gggtaatgga gatccccccg tattgctgtt attctggaag tgtttttaat
      901 attcgtattg atagttctca gagatatatc acactctaga tattttaatt aagtatcagt
      961 tcgatatcgg catattgtac actacctcag gtagatgatg agatcagata cagggatcaa
     1021 ctgacggtga gcgcagtccc cacattttat ccagggctta ctgcatattc ccaacagcca
     1081 ttcgttagcc aggtagacga ataccctgat ttgccgctgg ttttgctttc ctatcgaagg
     1141 gcccatacat catcacgccc acgaaacaac cgacgaaata acgcaacttt ctcatccgtg
     1201 gataatacga aaacgcactg cacagggctc tgcggtttac gtcgccattc aatcccatga
     1261 gcttcaagta atgagaccag ccgggtattt tctgcccgca gtactgctag ttcatcgctg
     1321 tcagatttat aaaccatatg cgttatcact tgtacctgtc tcccggcggc accacgactg
     1381 aaattcgtgt gaaggcaaat ccacgcgata acttacgata gttcggacat aagcccccat
     1441 aattcagtta tgtcgtggtc gaccccggtt acttttttcg ggaagcctgc tacggcccag
     1501 aataataccg tgttctttct gatcctgttt agccagatag ctaagggcgt aacgtaagtc
     1561 gtctaccgca gatttatcac tatagtgaat cacgtgatcg atacgcaccg ggtatttgtc
     1621 tttagcccgg cacagatgaa aataaccctc cccttccgtg atccgcctcc agatatctcc
     1681 cagttgcctg gatatctgat aagagttctt gtgacgttgt ccatccaggt agccgataaa
     1741 atgaatatga agccctttgt ctggtgtata ttccattacc cagtaatatc ccgccaacat
     1801 cgtctgggtt tcgctaagta aacggtatat ttccatacac atactgtgct tacaggaatg
     1861 cccgaaactg ggcgtgtctt tcctgtaagc aaaatcaatt ctgaaaggta acagtttaga
     1921 aaaacgttga aacataccat ccatgtgttc attcacgtct ttcagaatca tgaaatccat
     1981 tttgtaattg ggattggcat tatacatttt atagtcctta ctttataaaa ctcacaggaa
     2041 gagagaatta cgtaaatact gagagtagag aaacgccgta ccacggcatt aataaacgaa
     2101 gtaaaccagc agcacagaca acctgataaa actgttttgt attaccgcat tactgtgatg
     2161 aggtaatact tcagaataca gtgagactct cttaaactga gaaccagtac gaattacaat
     2221 ggaaaaacac acctcataga attgagtttc aggaagcacc actaaattct ttacaggaag
     2281 taatctgaca ttcacaaaac tcagtatcgg ctagctacag gcaacatcgc tattaatatt
     2341 gtcgaaccat taagtaaagc cgagagagac cacgggtatt taatcattag cataaaagaa
     2401 taatggtata cagaacatac ccacacacca taagaagatt gttccataca gaaaagatca
     2461 tatttaccta tacctataaa ctgtaacgga gtcataacac gctctaatag tgttccgata
     2521 cgtaaccagt gttaagtacg tacaggacaa cattacacac ataattcacc atacagcttc
     2581 ccccatatgc ttcactgcgt agtgtatgga gatatatgca ttacacacat acagaatact
     2641 tagcataaag aggagtgaca ttaccacgta aagagataag ttatacaaca aacatacaaa
     2701 caatacggca cagacacaca ataccataac tccaactaat aatactaatt aaccttaatt
     2761 atttatggta tatgaaataa atgtgattct cccggaacta actaacctaa ccagacagat
     2821 gatcactgta taaaaacttc agttaatact catttgtttt gtcgactcag tatataatga
     2881 gtgaccagta atcaggataa tcacacttac ataacctgta aaaaagaaca agaagaataa
     2941 gccactaaca taatgcaaaa tggcatctga atcgatcgca gtttggcatt cgaatcgatc
     3001 atgatttggc ccaatccgtg atcgaataga gcggaatacg caacataatt caccagacaa
     3061 gagcttacac cgaattacag attatgtatt aatgtcaaaa ctggcatatt ttacagatac
     3121 aatgaacatg caacccatca atctaatagg ggcggtatgt acatctgccc atgaatggga
     3181 tggcgctaag aatacaaaac aattagacat agatcacaaa aacacaccat tctaattgaa
     3241 aaacaaaaca aacaaataaa tcatactatt atagaatttt ctcttgccag acatagataa
     3301 tacccctgct aattttgcta atggttagca aaattagcag ggtatataaa gattattgac
     3361 ccaaaacaca ctcagatgaa ttcacgacag ctcaggaggg ttattcctga tgtcatcttc
     3421 acaaataatg gttaacgcat catatatcat tctgcgtctg ttcctgtttt cggccttatc
     3481 gccatacaac atcatcacat ttttaatttc atcgaccggt gatttcacat agtgaactaa
     3541 tacaccaagc ttctcaacac attgcagtaa agtatatttc ttactgcctg tgaaaagccc
     3601 tatcatagtt tctttacgtt cgcgactgga taatactttg ttttccataa tgacttctgc
     3661 cagcgtctta ccatcataaa catactcttt atctatcaga atatttatct tatccagcac
     3721 cttctctacg tcatcaagca aacctgtaat atatgggggc gcaatatatg gtttcgtact
     3781 gatcttttca agcatacaat tatacatctt aataacacgc atcagggtaa aattgttcgc
     3841 atcatactgt atttcaccct ccttatatac aggatcaata agtggtattt tttcaataaa
     3901 tgcatcctga taggctttta catggtcaag ccaataaaac aacgagccat actttatatt
     3961 ttttctattt actttaacct ccagtgctgt ccgaagcaac gataatgccc ccctgacata
     4021 tcctgaaggt aattcaccaa acaatccttt tctttccatg tcatcataat gctgcagcac
     4081 atcattaatt ttatttttcc ttatcagaca aatgtacacc gcaagtgttt cgtagcagct
     4141 ggtcacatga acagcctcat tctgcaactg cccaaaatag cttataatct ttttcagata
     4201 tgcgtcatca caaccattct tatcattaat attaaataca agatctttca gacgggaaat
     4261 atattcctga gatttatcat caagtgaaac actataacga gacaatgttc cttctgcact
     4321 gttttgaaac ttataaaaca tttccagtaa tttttttgca taaggcgttg tatcaaagct
     4381 ggtaaagtca taaagatatt gcgcagcgta accatacaaa ccatgaatcc agagtccttc
     4441 tctcaccaac actctgagat aatatgcaag gctttgaagt atcttcgtct ttcgttgtat
     4501 atcaagtaag acacttaatg aataaaatgc attatagaat ttatcaatat tcctgagcac
     4561 tttctccagt tcatccttat ccagcttctt ctcccggtaa tcctttttat attcattttt
     4621 gatagccatg atatattgtt gcatcaggct cttgcttttc ccgttatcct tccgtatagt
     4681 tcctgtatat ttccccagag catacagctt tccattttca tgaaaatctt ccttgttgaa
     4741 tcccagttca gccagatatt cccgaagata acgtttaaca gcagcttcat cgctttctgt
     4801 atcagcgtga tcaagacaag gacaatacga tttctgtaaa aaattataaa tatgataata
     4861 aaaagcagag tcattctctg gtctgtatgt attatacata tctacaacaa gaaaaataac
     4921 cccaaaagtg cgcagacaat agctgcgaag gaattcaggt tttagtgtgg aatgcactgg
     4981 ataagaattc atcatgtaat aaaaatgttc atatcgggca tatatgatcc gccgttgctc
     5041 ctgactgaac accccttcag catcaataat acgactgatc tcattataac gttgatccca
     5101 gaatttttga tgcatttcaa aattggtact cttccgcttg tgtgccggat tctcgccttc
     5161 cttaaacaac cccaaaacag tacaaatatc atacaacata tttgtaaatg tgcgcatatg
     5221 ctgtgctctc cggttggttt gcattttatc gcagaggaca ttctgagctt aacgtatcaa
     5281 actgacacaa cgacatgcaa tgcatatgga gaacaatatg tcaaatcaaa ccggacagct
     5341 ttacacgcct tcacgcatcc tgcgtatggc tgaacttacc cgactgctgg gtatatcaag
     5401 atctactatt tacgtgaaaa taaatccggc ctcgaaatac cacgacccta cctttcccaa
     5461 accaataagg cttggtagtg catccgtagg ctggcgggca tctgccatcg atgagtggct
     5521 gatgttacat acggcaccag cctggtctga acctgaaaac aaactaaagg aacttaatga
     5581 tgacgacaaa actatcactc cctgaacttg acacaactaa actgcctgac aggtgccagg
     5641 cattacttga tgaaatgcat gaagaaacag gaatacgccg tgaaatactt ctgtctgtta
     5701 tgctgacggt catggctgcc tcggtccagg atacgcatga agttgaactt tccggagggc
     5761 aacgtaccag ccttcagata tttatgtgtc tttcatcagc atcaggcagt ggaaaaacct
     5821 ctgcctgcgc aaaattaatc gctcctgttc atgaaacaga agaagaacta catcaggcat
     5881 atatcgacga taaaaaaaac tatgaccgaa tgatggaaat gtggacaaca gataaaaaaa
     5941 tcctggagcg gatatataaa aaggaaattg aaagatcccc ggaaaatgct gctgctgcac
     6001 gagcagcact tgaaaagtgt atagcaaata aacccgaacc tcccgtacaa caggttctca
     6061 tcgtgaatga cgcgacacca gaagggatag ctttgaaact cagccagtca ccctctcttc
     6121 tgttgctgtc tgatgaagga ggtactattc tggataagcg tttcgaacgc aaatcggcac
     6181 tgtacaacac attatggagt gggcaaccag tcaacgttga acgggcatcc agacccgggt
     6241 tccggctgaa tgatgtccgt ctcactatac ttatactgac tcaaccggta atatttaata
     6301 agttttttac tctcactggc gatcagatcc ggggcaatgg ctttctggcc cggttgctat
     6361 tttgcgaacc cggagcaatt aaaataatga cgacagaacc gcatactgcc actcctgttg
     6421 tgacgcaaca atgtgcaagc tgttttcgta agaaaagttt tggcagtcag atcagagact
     6481 ctctgagagc atccagagaa agacgtgcaa aaagtaaaga gcgcatctgc atgacattat
     6541 caaatgcagc atcccgcgca ctggagcgct ttcacaaaga aaatatggat actgtcagac
     6601 agaatcctca tatgggtaca tttgaagaca ttattgtcag aaaaagagag caggttgtca
     6661 ggatagcagc acttctggaa ctggagaaag atccagactc gacagtaata acgctggaaa
     6721 gtactaatag cgccatttat ctcattgaat tttatttcaa gcatcttata tataaacttg
     6781 aatcccttcg ggaaatatcc ccggctgaga aacttgataa atggcttcag aaaagaatta
     6841 tcacaacagc aggttacata ttccagaaaa gttatatttt acaatatgcc ccatatgctc
     6901 tcaggaaaaa atgtgttctg gatgaagcac ttgacatact ggcggaacaa aggaaaataa
     6961 ggattgatga taacctggtt gtttatatag gaaacacgat tacaccatcc gaactggcaa
     7021 aaaaactaaa tattcctgca ttcgatgccg gagttttcat ttgtgatcat caaaacatac
     7081 tgaagtatca ccgaaacaga ctatagatac tatcggacaa aaaataaatt atcacaagtg
     7141 tattattcgt tataataatg aataatcgtt atctgagtgc aatgccaggt tttcattacg
     7201 aactggcatt gcatttattt ttcaaaaaag aaagcagtaa ccatttggct actctgagta
     7261 tgccagtcca tgaaactaag cgaattcctt agtgttttta gcaagttgag acatcacata
     7321 atccgcccat gcctgcatca tcggtacacg ctgctccagt agatcagtac gatgatatgc
     7381 cgcctcaacc ttatttttca gcgtatgagc gagcgccctt tccgccagat cccgcgaata
     7441 cccctgttca ctacaccagt ccctgaatgt tgagcgaaaa ccatgtgccg tggcaactcg
     7501 ccccggaatg tcactgacgg ctttcttttt acgcagaaaa cttgtcaaca ccatatcgga
     7561 aaggatctgc tgctttctgg gtgaagggaa caccagttca tcatgcaggc cacgtatatt
     7621 ttccagaatg taaatagcct gccgggataa aggaacacga tgctgtagcc tagctttcat
     7681 tctttctgca ggtatagtcc atacccgctt atgaaaatca atttcagccc agcgcattcc
     7741 cctggcttcg cccgagcgag ttgctgtaag tatcaccatt aataacagtg cgcgggtaac
     7801 attataaggt tcatcactat acacactggt cgccacaaaa agcggtaact gcctccaggg
     7861 cattgcgggt tggtgttcat cacgtcctct tgtctgctga ggaagcaaat ggtcaaccac
     7921 atcaacagga tttgccacac aaaaaccgtg cgcccatccc cactgcataa caacatgaat
     7981 gcgctgttta acccggcttg ccgtttctga caaggttaac cagactggac gcagtgtttc
     8041 tgccacgtcc gcagccgtaa tcgaatccag cgtttttgct cccagttgag gaaacgcgta
     8101 attctcaagc gtcgataacc actgccttac atgctttgga ttctcccatc caggagacag
     8161 ttctgcatgt acacgcctgg ctgcatcggc aaatgttggg ataacgactt tctcagattc
     8221 agccttttta atctccagag gatcatcacc tgcagcaagt tgctctcgca ttatccgggc
     8281 agtacgtgca gcttcagcaa tactgacctc tgggtaagtt cccaatccag cattacgtct
     8341 tttttgtgtc accggactta cataacgaaa aacccatttc ccccgcccct ttactgaaga
     8401 aggatgaagg gtcagtccgg taattccccc atggggcaat ggtttgtcat caggtttgat
     8461 atgtcttgct ttcgtatccg tcaatactgc catacgctaa ttcccgtcac tctggtatgc
     8521 catccagtat gccattagtc cctcgcttca ctaagaatac attagataac atcggacaac
     8581 aaaaagtgta atacattgtt tataaagaaa ttatataaca cccctggatt aagtcagatt
     8641 tatttcaggc gg
//
LOCUS       DAJTMC010000170         8635 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.170, whole genome shotgun sequence.
ACCESSION   DAJTMC010000170 DAJTMC010000000
VERSION     DAJTMC010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8635
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.170"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            <1..66
                     /locus_tag="OO130_003515"
     CDS             <1..66
                     /locus_tag="OO130_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HCR5366784.1"
                     /translation="RYQAKYVSAIWQKLIPSQADF"
     gene            125..328
                     /locus_tag="OO130_003516"
     CDS             125..328
                     /locus_tag="OO130_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HCR5366785.1"
                     /translation="MSSLFLTEDELLILTGCKYASHQRKWLMENELPFYTNRSGKPIV
                     SRDLFTCNKTLPPREVEPNFGAI"
     gene            328..1353
                     /locus_tag="OO130_003517"
                     /pseudo
     CDS             328..1353
                     /locus_tag="OO130_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105555.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(1381..1470)
                     /locus_tag="OO130_003518"
     tRNA            complement(1381..1470)
                     /locus_tag="OO130_003518"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1434..1436),aa:Ser,seq:cga)
     gene            1564..2361
                     /gene="mtfA"
                     /locus_tag="OO130_003519"
     CDS             1564..2361
                     /gene="mtfA"
                     /locus_tag="OO130_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HCR5366786.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFIPLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            2462..2537
                     /locus_tag="OO130_003520"
     tRNA            2462..2537
                     /locus_tag="OO130_003520"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2495..2497,aa:Asn,seq:gtt)
     gene            2851..>8635
                     /locus_tag="OO130_003521"
     CDS             2851..>8635
                     /locus_tag="OO130_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HCR5366787.1"
                     /translation="MATKKRSGEEINDRQILCGMGIKLRRLTAGICLITQLAFPMAAA
                     AQGVVNAATQQPVPAQIAIANANTVPYILGALESAQSVAERFGISVAELRKLNQFRTF
                     ARGFNNVRQGDELDVPAQVSEKKLTPPPGNSSDNLEQQIASTSQQIGSLLAEDMNSEQ
                     AANMARGWASSQASGAMTDWLSRFGTARITLGVDEDFSLKNSQFDFLHPWYETPDNLF
                     FSQHTLHRTDERTQINNGLGWRHFTPTWMSGINFFFDHDLSRYHSRAGIGAEYWRDYL
                     KLSSNGYLRLTNWRSAPELDNDYEARPANGWDVRAESWLPSWPHLGGKLVYEQYYGDE
                     VALFDKDDRQSNPHAITAGLNYTPFPLMTFSAEQRQGKQGENDTRFAVDFTWQPGSAM
                     QKQLDPNEVAARRSLAGSRYDLVDRNNNIVLEYRKKELVRLTLTDPVTGKSGEVKSLV
                     SSLQTKYALKGYNVEATALEAAGGKVVTTGKDILVTLPAYRFTSTPETDNTWPIEVTA
                     EDVKGNLSNREQSMVVVQAPTLSQKDSSVSLSTQTLNADSHSTATLTFIAHDAAGNPV
                     VGLVLSTRHEGVQDITLSDWKDNGDGSYTQILTTGAMSGTLTLMPQLNGVDAAKAPAV
                     VNIISVSSSRTHSSIKIDKDRYLSGNPIEVTVELRDENDKPVKEQKQQLNNAVSIDNV
                     KPGVTTDWKETADGVYKATYTAYTKGSGLTAKLLMQNWNEDLHTAGFIIDANPQSAKI
                     ATLSASNNGVLANENAANTVSVNVADEGSNPINDHTVTFAVLSGSATSFNNQNTAKTD
                     VNGLATFDLKSSKQEDNTVEVTLENGVKQTLIVSFVGDSSTAQVDLQKSKNEVVADGN
                     DSATMTATVRDAKGNLLNDVMVTFNVNSAEAKLSQTEVNSHDGIATATLTSLKNGDYR
                     VTASVSSGSQANQQVNFIGDQSTAALTLSVPSGDITVTNTAPQHMTATLQDKNGNPLK
                     DKEITFSVPNDVASRFSISNSGKGMTDSNGVAIASLTGTLAGTHMITARLANSNVSDT
                     QPMTFVADKDRAVVVLQTSKAEIIGNGVDETTLTATVKDPFDNVVKNLSVVFRTSPAD
                     TQLSLKALNTNENGIAEVTLKGTVLGVYTAEAILLNGKSDTKIVNIVPDTSNAQVTLN
                     IPAQQVVTNNSDSVQLTATVKDPSNHPVAGITVNFTMPQDVAANFTLENNGIAITQAN
                     GEAHVTLKGKKAGMHTVTATLGNNNASDAQPVTFVADKDSAVVVLQTSKAEIIGNGVD
                     ETTLTATVKDPFDNAVKDLQVTFSTKPADTQLSQSTSNTNDSGVAEVTLKGTVLGVHT
                     VEATLPNGNGYTTTVNIAPDTSNAQVTLNIPAQQVVTNNSDSVQLTAMVKDPSNHPVA
                     GITVNFTMPQDVAANFTLENNGIAITQANGEAHVTLKGKKAGTHTVTATLGNNNTSDS
                     QPVTFVADKTSAQVVLQMSKDEITGNGVDNATLTATVKDQFDNGVNNLPVTFSSASSG
                     LTLTPGVSNTNESGIAQATLAGVAFGEKTVTASLANNGASDNKTVHFIGDTAAAKIIE
                     LTPVPDSIIAGTPQNSSGSVITATVVDNNGFPVKGVTVNFTSNAATAEMTNGGQAVTN
                     EQGKATVTYTNTRSSIESGARPDTVEASLENGSSTLSTSINVNADASTAHLTLLQALF
                     DTVSAGETTSLYIEVKDNYGNGVPQQEVTLSVSPSEGVTPSNNAIYTTNHDGNFYASF
                     TATKAGVYQLTATLENGDSMQQTVTYVPNVANAEITLAASKDPVIADNNDLTTLTATV
                     ADTEGNAIANTEVTFTLPEDVKANFTLSDGGKAITDAEGKAKVTLKGTKAGAHTVTAS
                     MTGGKSEQLVVNFIADTLTAQVNLNVTEDNFIANNVGMTRLQATVTDGNGNPLANEAV
                     TFTLPADVSASFTLGQGGSAITDINGKA"
BASE COUNT         2470 a         2070 c         2107 g         1988 t
ORIGIN      
        1 cgttaccagg caaaatacgt ttcagctatc tggcaaaaac tgatcccgag tcaggctgat
       61 ttttaatgtt caaccctaat tgccgctaac cgtatatagt tagcggcggt tatgagatat
      121 agctatgagc agcttatttt taaccgaaga tgaattgcta atattaacgg gctgcaaata
      181 tgcaagccac cagcgaaaat ggttaatgga aaacgagctt ccgttctata ccaatcgtag
      241 tggcaaaccg attgtcagcc gggatctatt tacctgcaat aaaactttac caccacgcga
      301 ggtagagccg aattttggtg cgatctgatg ggaagacgaa ggaaaaatcc tgagcacgaa
      361 aaattacctc caaatgtata cccaaataaa tatagttatg tatggaaacc aacatccagc
      421 gaatctgtaa cactaaccgc catcaaggat ggtttagctg ctttatggaa aaagtatgag
      481 gaaactgtaa ataatcgcga tcgtgcaatg acattcggtc gcttgtggga aaaattcctc
      541 gccagcgcct attacagtga cctcagtcca agaacacaaa aagattatct gcaacatcaa
      601 aaaagttgct tgccgtattc ggtaaggtgc cggcagattc cataaaacca gaacacatcc
      661 gtcgatacat ggacaagaga ggggaacaga gtaaaacgca agccaaccat gaagtcccgc
      721 gtttacagtt gggggtatga gcgagggtac gtgaaggcta acccatgtgc aggtgtaagt
      781 aaattcaagg ccaaaaaccg cgaacgatat gtaaccgaca aagaatacca ggcagtatta
      841 agcgttgcac ctcttcctgt ttttatcgca atggaaattg cctatctgtg tgcagcgagg
      901 gtttccgatg tgttatcgct gaaatgggag cagattggaa acgacgggat ctttatccag
      961 caagggaaaa caggaaaaaa acagataaaa gcatggagtc cacgattaca ggcggcgatc
     1021 gaaaaagcaa aacagttacc aacatccgcc tatgtaatca gcaatcaata cggcaaccga
     1081 tatatgtaca aaggctttaa cgaaatgtgg gtagaagcaa gaaatcgcgc aggcaaaatt
     1141 tcaggtattt taaccgactt cacctttcat gatctgaagg cgaaaggaat ttcagactat
     1201 gaaggaagca gtcgggataa gcaacttttc tctggtcaca aaaccgaggg gcaagtgcta
     1261 atctatgaca ggaaggttaa agtttcaccg acacttgatg tcccgttacc tgaaaatatt
     1321 ccaagtaatt ccacgtgtga tttttgtcac tgacttaatg atgtataagt gattgaattt
     1381 tggcggagag agggggattt gaacccccgg tggagttgcc cccactccgg ttttcgagac
     1441 cggtccgttc agccgctccg gcatctctcc gttcagatgg ttgccatgat gccaggaaat
     1501 ttggcatttt aacagtccct gtccgtgcaa ttttgttcaa gtgacgagtt tgcgagcaaa
     1561 acgatgatta agtggccctg gaaagtacaa gaatcagcac atcaaactgc ccttccctgg
     1621 caggaagcac tatcgatccc ccttttaacg tgtctgacgg aacaggaaca aagcaaatta
     1681 gtcactcttg ccgaacgttt tttacagcaa aagcggcttg ttcctttaca gggctttgaa
     1741 ctggattcat taagaagctg ccggatagca cttctatttt gcctacccgt tctggagtta
     1801 ggactggaat ggctggatgg ttttcatgaa gtcttaattt atcctgcgcc atttgtggtc
     1861 gatgatgaat gggaagacga tatcggtctg gtgcataacc aacgtattgt tcagtcaggt
     1921 cagagctggc agcaagggcc tatcgttttg aactggttgg atatacaaga ttcttttgat
     1981 gcttctggtt ttaacctgat tattcatgaa gttgctcata agctggacac ccgtaacggc
     2041 gatcgcgcca gcggagttcc ctttattccg ttgcgtgagg ttgctggctg ggaacacgat
     2101 cttcatgctg caatgaacaa cattcaggaa gaaatcgaat tggttggcga gaatgcggcg
     2161 agcattgatg cttatgcagc cagcgatcct gctgaatgtt ttgccgtact ttctgaatat
     2221 ttctttagcg ccccagaact ttttgctcct cgtttccctt cattgtggca acgtttctgc
     2281 caattttatc aacaagatcc tttgcagaga ctgcatcacg ctaatgatac agactcgttt
     2341 tcggcgacga atgttcatta attaacaact ttgcatatta attaatcaat tgaaatgact
     2401 tatgaaattt agtgttgaca gacaaggtac cgctaagtaa tatgcgcccc gttcacacga
     2461 ttcctctgta gttcagtcgg tagaacggcg gactgttaat ccgtatgtca ctggttcgag
     2521 tccagtcaga ggagccaaat tctaaaaatt cgctttttta gcgcaatgtc actgacctta
     2581 gttgaacatt gttttttaac ggatagcggg tttttaacat cttaagcgcc ctcgaccttt
     2641 atggttgagg gcgttttgct atgaacgcca tcaccatttt cccctcgatt ataaaacttg
     2701 agttattcag tagtctcccc tcttgcaact cacacccaaa actgcctaac gaaaagttat
     2761 taattttcaa tcatattgct atcagtattt acattttttc gctgtgctag aaagggcgca
     2821 tttatgttag ctcgttcagg gaaggtaagc atggctacga agaagagaag tggagaagaa
     2881 ataaatgacc gacaaatatt atgcgggatg ggaattaaac tacgccgctt aactgcgggt
     2941 atctgtctga taactcaact tgcgttccct atggctgcgg cagcacaagg tgtggtaaac
     3001 gccgcaaccc aacaaccagt tcctgcacaa attgccattg caaatgccaa tacggtgccc
     3061 tacatccttg gagcgctgga atcggcccaa agcgttgccg aacgtttcgg tatttcggtg
     3121 gctgagttac gcaaactcaa ccagtttcgt acgtttgctc gaggttttaa taatgtccgc
     3181 cagggtgatg aactggatgt cccggcacaa gttagtgaaa aaaaattaac cccgccgccg
     3241 ggtaatagca gtgacaacct cgagcaacag atagccagta cttcacagca aatcgggtct
     3301 ctgctcgccg aagatatgaa cagcgagcaa gcggcaaata tggcgcgtgg atgggcctct
     3361 tctcaggctt caggcgcaat gacagactgg ttaagccgct tcggtaccgc aagaatcacg
     3421 ctgggcgtgg atgaagattt tagcctgaag aactcccagt tcgattttct ccatccgtgg
     3481 tatgaaacgc ctgataatct ctttttcagt cagcatactc tccatcgtac tgacgagcgt
     3541 acgcagatta acaacggctt aggttggcgt catttcactc ccacatggat gtcaggcatc
     3601 aacttctttt tcgaccacga tcttagccgt taccactccc gcgccggcat tggcgcggag
     3661 tactggcgcg actatctaaa attaagcagt aacggctatt tgcgactgac caactggcgc
     3721 agcgcacctg aactggacaa cgattatgaa gcacgcccgg ccaatggctg ggatgtacgc
     3781 gcagaaagct ggctaccctc ctggccgcac cttggcggta aactggtcta tgaacagtat
     3841 tatggcgatg aagtggccct gttcgataaa gacgatcggc aaagtaatcc tcatgccata
     3901 accgctggac ttaactatac ccccttcccg ctgatgacct tcagcgcgga gcaacgccag
     3961 ggtaaacagg gcgaaaatga cacccgtttt gccgtcgatt ttacctggca acctggcagc
     4021 gcaatgcaga aacagcttga cccgaatgaa gtcgctgcac ggcgtagcct tgcaggcagc
     4081 cgttatgatc tggtggatcg caacaacaat atcgttctgg aatatcgcaa aaaagaactg
     4141 gttcgcctga ccctgacaga ccccgtgaca gggaagtcag gagaagtgaa atcactggtt
     4201 tcgtcgctac aaaccaaata tgccctgaaa ggctataacg tcgaagccac cgcactggaa
     4261 gctgccggtg gcaaagtggt cacaacgggt aaagatattc tggttaccct gccggcttac
     4321 cggttcacca gtacgccaga aaccgataac acctggccga ttgaagtcac cgccgaagat
     4381 gtcaaaggca atttgtcgaa tcgtgaacag agcatggtgg tcgttcaggc acctacgcta
     4441 agccagaaag attcctcggt atcgttaagt acccaaacat tgaacgcgga ttcccattca
     4501 accgccacac tgacttttat tgcgcatgat gcagcaggta atcctgttgt cgggctggtg
     4561 ctctcgacgc gtcacgaagg tgttcaggac atcacccttt ctgactggaa agataatggt
     4621 gacggaagct atacccagat cctgaccaca ggagcgatgt ctggcacgct gacgctgatg
     4681 ccacagctga atggtgtgga tgcggctaaa gcccccgccg tggtgaatat catttctgtt
     4741 tcgtcatccc gaactcactc gtcaattaag attgataagg accgttatct ctccggcaat
     4801 cctatcgagg tgacggtaga actgagagat gaaaatgaca aacctgttaa ggaacaaaaa
     4861 cagcaactga ataacgcagt cagcatcgac aacgtgaaac caggagtcac tacagactgg
     4921 aaagaaaccg cagatggcgt ctataaggcg acctataccg cctataccaa aggcagtgga
     4981 cttactgcga agctattaat gcaaaactgg aatgaagatt tgcataccgc tggatttatc
     5041 atcgacgcca acccgcagtc agcgaaaatt gcgacattat ctgccagcaa taatggtgtg
     5101 ctcgccaatg agaatgcagc aaacaccgtc tcggtcaatg tcgctgatga aggaagcaac
     5161 ccaatcaatg atcataccgt cacgtttgcg gtattaagcg gatcggcaac ttccttcaac
     5221 aatcaaaaca ccgcaaaaac ggatgttaat ggtctggcga cttttgatct gaaaagtagt
     5281 aagcaggaag acaacacggt tgaagtcacc cttgaaaatg gcgtgaaaca aacgttaatc
     5341 gtcagttttg tcggcgactc gagtaccgcg caggttgatc tgcagaagtc gaaaaatgaa
     5401 gtggttgctg acggcaatga cagcgccaca atgaccgcga ccgtccggga tgcaaaaggc
     5461 aacctgctca atgacgtcat ggtcactttc aatgttaatt cagcagaggc gaaactgagc
     5521 caaaccgaag tgaatagcca cgacgggatc gccacagcta cgctgaccag tttgaaaaat
     5581 ggtgattata gggttacggc ctctgtgagc tctggttccc aggctaatca acaggtgaat
     5641 tttatcggtg atcaaagtac tgctgccctg accctcagtg tgccttcagg tgatatcacc
     5701 gtcaccaaca cagctccgca acatatgact gcaaccttgc aggataaaaa tggcaaccca
     5761 ctaaaagata aagaaatcac cttctctgta ccaaacgacg tcgcaagtcg gttctcgatt
     5821 agcaacagcg gaaaaggcat gacggatagc aacggggttg caatcgcctc cctgaccggc
     5881 acgttagcgg gcacgcatat gatcacggct cgtctggcta acagcaatgt cagcgataca
     5941 cagccaatga cgtttgtggc ggataaagac agagcggttg tcgttctgca aacatcgaaa
     6001 gcggaaatca ttgggaatgg cgtggatgag acaactctga cagcaacagt gaaagatcct
     6061 tttgataacg tggttaaaaa tctgtcagta gtcttccgca cctcccccgc agacacgcaa
     6121 ctgagtctga aggcgcttaa tactaatgag aacggtattg ctgaagttac ccttaagggc
     6181 acggttttgg gtgtttatac agccgaagcc atactgctta acggtaaaag cgatacgaaa
     6241 atcgtcaata ttgtgcccga taccagcaac gcgcaggtca ccctgaacat cccagctcaa
     6301 caggtggtga cgaataacag cgacagcgtg cagctgacgg cgacggtgaa agatccgtcg
     6361 aatcatccgg tggcgggaat aacggtgaac ttcaccatgc ctcaggacgt ggcggcaaac
     6421 tttacccttg aaaataacgg tattgccatc actcaggcca atggcgaagc gcatgtcacc
     6481 ctcaaaggta aaaaagcggg catgcatacg gttaccgcaa cgttgggtaa caataatgcc
     6541 agcgatgcgc aacctgtcac attcgtggcg gataaggaca gcgcagttgt cgttctgcaa
     6601 acatcgaaag cggaaatcat tgggaatggc gtggatgaga cgactctaac agcaacagtg
     6661 aaagatcctt ttgataacgc agtaaaagat ctacaggtta ccttcagtac caagccggca
     6721 gatactcaac ttagtcagag cacaagcaat actaacgaca gtggtgttgc tgaagttacc
     6781 cttaagggca cggttttggg cgttcataca gtcgaagcca cacttcctaa cggcaacggc
     6841 tatacaacga cagtcaatat tgcgcccgat accagcaacg cgcaggttac gctgaacatc
     6901 cctgctcaac aggtggtgac gaataacagt gacagcgtgc agcttacggc gatggtgaaa
     6961 gatccgtcga atcatccggt ggcggggata acggtcaact tcaccatgcc acaggacgtt
     7021 gcggcaaact ttaccctcga aaataacggc attgccatca cccaagccaa tggggaagcg
     7081 catgtcacgc tgaaaggtaa aaaagcgggt acgcatacgg ttaccgcaac gctgggtaat
     7141 aacaatacca gtgattcgca gccggtaacg tttgtggcgg acaaaacctc ggctcaggtt
     7201 gtcctgcaga tgtcaaaaga tgagatcaca ggtaatggcg tcgataacgc aacgctaact
     7261 gcaacggtta aagatcagtt cgacaatggg gtgaataatc ttccggtaac attcagctca
     7321 gcctcttcag gactcaccct gaccccggga gtaagtaata ccaatgagtc tggcatcgcg
     7381 caggccactc tcgcaggcgt tgcctttggt gagaagacgg ttactgcatc actggctaat
     7441 aatggtgcca gcgacaacaa aactgtgcat tttattggcg acacagcggc ggcaaaaatt
     7501 atcgagttga cgcctgtccc agacagcata atcgccggta ccccgcagaa cagctccggc
     7561 agcgtcatca ccgccacagt cgttgataat aatggctttc cggtgaaagg tgtgactgtg
     7621 aacttcacca gcaacgcagc gacagccgaa atgacgaacg gcggtcaagc cgtgacgaac
     7681 gaacagggta aggctaccgt cacttatacc aatacccgct cctcgataga atcaggagcg
     7741 agaccggata ccgttgaggc cagtctggaa aatggtagct ccacgcttag cacatcaatt
     7801 aatgtcaacg ctgatgcgtc tacggcacat ctcaccttgc tacaggcact ttttgataca
     7861 gtctccgcag gcgagacaac cagtctgtat attgaggtga aggataatta cggcaacggt
     7921 gtcccccagc aggaggtaac cctcagcgtt tcaccaagtg aaggcgtgac ccccagtaat
     7981 aacgctatat atactaccaa ccacgacggc aatttttacg caagctttac cgctacaaaa
     8041 gccggggttt atcaattgac ggcaaccctc gaaaatggcg attcgatgca acaaacagtg
     8101 acctatgtgc cgaacgtcgc gaatgctgaa atcacgctgg cagcctcgaa ggatccggtg
     8161 attgccgaca ataacgatct cacgacacta acagcaacag tcgctgatac agagggcaat
     8221 gcgatagcca acactgaggt aacatttact ctgccggaag atgtgaaggc gaacttcacg
     8281 ctgagcgatg gcggtaaagc gattactgat gctgaaggca aagcgaaagt cacgctgaaa
     8341 ggtacaaaag caggcgctca tactgttaca gcatcgatga ctggcggtaa gagtgagcag
     8401 ttggtggtga actttattgc ggatacgctc actgcgcagg ttaatcttaa cgttaccgag
     8461 gacaatttta tcgctaataa cgtcgggatg accaggctgc aggcaacagt gactgatgga
     8521 aacggcaacc cgttagccaa tgaggcggtg acattcacgc taccggcaga tgtgagcgca
     8581 agctttactc tcggacaagg cggttccgcc attactgata tcaacggcaa ggctg
//
LOCUS       DAJTMC010000171         8621 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.171, whole genome shotgun sequence.
ACCESSION   DAJTMC010000171 DAJTMC010000000
VERSION     DAJTMC010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8621
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.171"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            <1..190
                     /locus_tag="OO130_003522"
     CDS             <1..190
                     /locus_tag="OO130_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5366788.1"
                     /translation="DMLNPTKDTNWNSTCIYKSRHKMLPVNLTQETLFNSKSQDKHAL
                     FPIFTASWRAYRIMNKGA"
     gene            413..910
                     /gene="ubiC"
                     /locus_tag="OO130_003523"
     CDS             413..910
                     /gene="ubiC"
                     /locus_tag="OO130_003523"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HCR5366789.1"
                     /translation="MSHPALTQLRALRYFKEIPALDPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            923..1795
                     /gene="ubiA"
                     /locus_tag="OO130_003524"
     CDS             923..1795
                     /gene="ubiA"
                     /locus_tag="OO130_003524"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HCR5366790.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSILVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(1950..4373)
                     /gene="plsB"
                     /locus_tag="OO130_003525"
     CDS             complement(1950..4373)
                     /gene="plsB"
                     /locus_tag="OO130_003525"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HCR5366791.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPSHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            4544..4912
                     /gene="dgkA"
                     /locus_tag="OO130_003526"
     CDS             4544..4912
                     /gene="dgkA"
                     /locus_tag="OO130_003526"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCR5366792.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            5022..5630
                     /gene="lexA"
                     /locus_tag="OO130_003527"
     CDS             5022..5630
                     /gene="lexA"
                     /locus_tag="OO130_003527"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HCR5366793.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            5703..7028
                     /gene="dinF"
                     /locus_tag="OO130_003528"
     CDS             5703..7028
                     /gene="dinF"
                     /locus_tag="OO130_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HCR5366794.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHALWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            7144..7353
                     /locus_tag="OO130_003529"
     CDS             7144..7353
                     /locus_tag="OO130_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCR5366795.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(7395..7910)
                     /gene="zur"
                     /locus_tag="OO130_003530"
     CDS             complement(7395..7910)
                     /gene="zur"
                     /locus_tag="OO130_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HCR5366796.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQSTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            8228..8621
                     /locus_tag="OO130_003531"
                     /pseudo
     CDS             8228..8621
                     /locus_tag="OO130_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313057.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 family protein"
BASE COUNT         2033 a         2046 c         2310 g         2232 t
ORIGIN      
        1 tgatatgttg aatcctacaa aagataccaa ttggaattct acgtgtatct ataaatccag
       61 acataagatg ttgcctgtta atcttactca ggaaacactt ttcaactcca aatctcagga
      121 taaacatgcg cttttcccca tttttactgc gagttggcga gcttatcgta taatgaataa
      181 gggtgcttaa gtaaaggaaa acatcaccgt tcctggcatc ctgggcggtg atgcccctat
      241 ggttgccctc gccagcgcgg gcatcggcaa agcgtaaggt tcaaaagcga tttaccactt
      301 catgcgattg ttgcgttttt gttgcgcatt agatcactta atttgcttta catctcccgt
      361 aaacactttt ctgcgataca atgcctttac gttatgtaac ggagagttcg gcatgtcaca
      421 ccccgcgtta acgcaactgc gtgcgctgcg ctattttaaa gagatccctg ccctggatcc
      481 gcaactgctc gactggctgt tgctggagga ttccatgaca aaacgttttg aacagcaggg
      541 aaaaacggta agcgtgacga tgatccgcga agggtttgtc gagcagaatg aaatccccga
      601 agaactgccg ctgctgccga aagagtctcg ttactggtta cgtgaaattt tgttatgtgc
      661 cgatggtgaa ccgtggcttg ccggtcgtac cgtcgttcct gtgtcaacgt taagcgggcc
      721 ggagctggcg ttacaaaaat tgggtaaaac gccgttagga cgctatctgt tcacatcatc
      781 gacattaacc cgggacttta ttgagatagg ccgtgatgcc gggctgtggg ggcgacgttc
      841 ccgcctgcga ttaagcggta aaccgctgtt gttaacagaa ctgtttttac cggcgtcacc
      901 gttgtactaa gaggaaaaaa atatggagtg gagtctgacg cagaataagc tgctggcgtt
      961 tcatcgctta atgcgtacgg ataagccaat tggcgcgtta ctgctgctct ggccaacatt
     1021 atgggcgttg tgggtggcga caccgggcgt tcctcagctc tggatcctgg cggtatttgt
     1081 cgcgggtgtc tggctgatgc gcgctgccgg atgtgtggtg aatgattatg ctgaccgaaa
     1141 gtttgatggt catgttaagc gcacggcgaa ccgaccactt cccagcggcg cggtaacaga
     1201 gaaagaggcg cgcgcgctgt ttgtagtgct ggtactgatt tcgtttttac tggtactgac
     1261 gctgaatacg atgaccattc tgttgtcgat tgccgctctg gcgttggcgt gggtgtaccc
     1321 gtttatgaag cggtataccc atctcccgca agtggtgctg ggcgcggcgt ttggctggtc
     1381 gattccaatg gcttttgccg ctgtgagtga gtcggtgcca ttgagttgct ggttaatgtt
     1441 cctcgccaat attctctggg cggtggctta cgacacgcag tatgcgatgg ttgaccgcga
     1501 tgatgatgtg aagattggca ttaaatccac ggcaatcctg ttcggccaat acgataaatt
     1561 gattattggt attttgcaga ttggcgtact ggcactgatg gcgatcatcg gtgagttaaa
     1621 tggcttaggc tggggatatt actggtcaat tctggtggct ggcgcgctgt ttgtttatca
     1681 acaaaaactg attgccaacc gcgagcgtga agcctgcttt aaagcattta tgaataataa
     1741 ctatgttggt ctggtactat ttttagggct ggcaatgagt tactggcatt tctgatgatg
     1801 taaaaaagcc ggatgatcat ccggctttct tctgggttgc ctgatgcgcg gcgcttctca
     1861 ggcctacaca acacatcgca atttattgaa tttgcagatt atggaatgcc ggataaggcg
     1921 ttttcgccgc atccggcaat tctctctggt tacccttcgc cctgcgtcgc actctcaatc
     1981 gtcaaacgca cgtctgatgt aatcaactcc gccagcaact gataaacctt catcgtttct
     2041 gccggttcgg catcgccgct atcgctgata tacccttcat cacgcagcgt cagcaccaga
     2101 gaactgaaca ccgccttgtc gaagaactcc ggcgcgttga taccgtgcag cacggagaga
     2161 cgttgcgcga cggtgcggct ctctttctcc agcgtaccgc ggttgatcga cgggttagcg
     2221 ctcaacagcc agaaggtgat ggcataacgt tgcagcgttt cgcgcgcgcc tgcggccagc
     2281 agctgtagcg tgcgagaatg cgacgggttg atatgcaact catcatcttg cagggtaatc
     2341 agcccctgac gttgcatctc atttgccaac gcatcaataa cgtccggcaa ctcgtcgcga
     2401 tcccagcgca ggaacagctc cgctttcagc attgggtaaa gcacattgac gtgctccatc
     2461 aatacgtcgc gggagatgtg gcgatgctgg gtgacaattg ccgccatcag cgaaggcagc
     2521 accaacatat gcgcaatgtt gttgcgatag taggtcatca gcaccgcttg ctcgcgcggc
     2581 agaatgatga tgtcgccgat agtgtctttc tcaacttcaa acttgttcat ttgcagcgcg
     2641 tgatcgataa gctcgctggc gctggctgaa ggaacggtag agtccgtgga gtaaggtacg
     2701 ttgcgcatca gatccaggta gcagttgagt tgctcggtta actgctcgcg ggtaagtgag
     2761 cgctgacgtg atgccagtag cgcggtacag cagaggttca tggcgtttgc cgcgcctgcg
     2821 ttgttaatgc gtaccatcag atcggcagca atattattga ccgtcggcgt taaccatgcc
     2881 ggacgcaccg cttcgatggg atcgatagat tcacgccagt caggtacatg ctggttaagg
     2941 taggtcatca acggcattgg ttcaccgaag ttgacgtaac cctgaccgag attacgcagc
     3001 ttgcttaaac cgcgcagcat ctgcggcagg ctctctttct ctttcgtcgc accgcgcagt
     3061 tctttggcgt aagtacccac ttccatgacg tgctcataac cgatatagat cggaatcagc
     3121 gtaatcggac gcgtgccgcc acgcagcatc gcctgaatgg tcatcgacag cgtaccggtt
     3181 ttcggatcca gcaaacgccc cgtacgggaa cgaccgcctt ccacgaaata ctcgacggaa
     3241 taaccacggc tgaacagttc gccgagatac tcacggaaaa cggtggaata aagtttattg
     3301 cctttaaacg tacggcgaat aaagaacgcc cccagacggc ggaaaatcgg cccggccggc
     3361 cagaaattca ggttgatccc ggcggcgata tgcggcggca ccagcccctg gtggtacagc
     3421 acgtaagaaa gtagcaggta gtccatgtga ctgcggtggc aaggcacata caccagctca
     3481 tgaccgtcgt gggccagctg gcgaacgcgc tcggcgttat ggacgttgat gccctgataa
     3541 agtcggttcc aggtgaagcc cagaatgcgg tcagtcaggc ggatcatctc gtaagagaaa
     3601 ttcgccgcaa tctcttccat cagcgcaatc gcgttctgct gcgctttttc atgggagatt
     3661 tttttgctgc gcgcttcatc ttctaccgct ttggcaatgg cgcgggaggc gagcagctta
     3721 ttaaacagat cctgacgagc aggcagacgt gggcccacag cagccagacg ttgacgggca
     3781 aagtgcatac gcgccacgcg cgccagtttc tgtgcgatag ttttatccgt gccgtgttca
     3841 tccgccatac ggcgcagcga aactgacggc gagaaacgca caaaactgtc gcgaccgagc
     3901 cacagtacgg cgaaaaattt ctgtacgccg ttaagcatac gcagcggcgg gtttacttcg
     3961 cctttttcac gccccggcgc gcgaccaaac atcaccgata ctggcaccat ctgcacatcc
     4021 agatttgggt tgctacggtg caaatcgaga tagtcgtgga acagcttaat agactcttct
     4081 ttcggcgtgt aataggtgaa tacacgcggc ccgccgtgaa tgaacacata gcgcggcagt
     4141 agcgtgccgt cgatttccag cggctctaac gggtcaggca agtcatgagc cagacactgg
     4201 gcgcgtaacg tcagcaaatc tgctttcgag ttgtacggta aaacgtacat aattggacga
     4261 gaggtatcca gccccagttc cggggcagga tctgccggaa tagacttgct ttttaccagg
     4321 atgcttaatg gtaaattcag taatttgtag taaattcgtg gccagccgga cataaacgat
     4381 gtaaagcctc tggttaataa tgcaaatgcg cggcaaggat agcagaaagt catgggaaat
     4441 tctgtggtat ccgctcatgt ttcgcgcggc gttacgcaaa cccgaatcat cggatttaac
     4501 ggtacactga tattgacgct cataatgtaa aaaggttctt tcaatggcca ataataccac
     4561 tggattcacc cgaattatca aagctgctgg ctattcctgg aaaggtttac gcgctgcatg
     4621 gatcaacgaa gcggcattcc gtcaggaagg cgtagcggta ttgttggcgg tggtcatcgc
     4681 ctgctggctg gatgtggacg caattacccg cgtgctgctt atcagctccg tgatgctggt
     4741 gatgattgtg gaaatcctca atagcgccat cgaagcagtg gttgaccgaa ttggctctga
     4801 ataccatgag ctttccggac gcgcaaaaga tatgggatcc gccgcggtgc tgattgcgat
     4861 tatcgtcgcc gtgataacct ggtgcatcct gttatggtcg cattttggat aacccttcca
     4921 gaattcgata aatctctggt ttattgtgca gtttatggtt ccaaaatcgc cttttgctgt
     4981 atatactcac agtataactg tatatacacc cagggggcgg aatgaaagcg ttaacggcca
     5041 ggcaacaaga ggtgtttgat ctcatccgtg atcacatcag ccagacaggt atgccgccga
     5101 cgcgtgcgga aatcgcgcag cgtttggggt tccgttcccc aaacgcggct gaagaacatc
     5161 tgaaggcgct ggcacgcaaa ggagttattg aaattgtttc cggcgcatca cgcgggattc
     5221 gtctgttgca ggaagaggaa gaagggttgc cgctggtagg tcgtgtggct gccggtgaac
     5281 cgcttctggc gcagcagcat attgaaggtc attatcaggt cgatccttcc ttattcaagc
     5341 cgaatgctga tttcctgctg cgcgtcagcg ggatgtcgat gaaagatatt ggcattatgg
     5401 atggcgactt gctggcagtg cataaaactc aggatgtacg taacggtcag gtcgttgtcg
     5461 cacgtattga tgacgaagtt accgttaagc gcctgaaaaa acagggcaat aaagtcgaac
     5521 tgttgccaga aaatagcgag tttaaaccaa ttgtcgttga ccttcgtcag cagagcttca
     5581 ccattgaagg gctggcggtt ggagttattc gcaacggcga ctggctgtaa catatctctg
     5641 agaccgcgat gccgccaggc gtcgcggttt gtttttcatc tctcttcatc aggcttgtct
     5701 gcatggcatt cctcacttca tctgataaag cactctggca tctcgcctta cccatgattt
     5761 tctccaatat caccgttccg ttgctgggac tggtcgatac ggcggtaatt ggtcatcttg
     5821 atagtccagt ttatttgggc ggcgtggcgg ttggtgcaac ggcgaccagc tttctcttta
     5881 tgctgttgct gtttttacgc atgagcacca ccgggctgac tgcgcaggct tatggtgcca
     5941 aaaatcctca ggcattagcc cgtgcgctgg tgcaaccgtt gctgttggcg ttgggggcag
     6001 gggcgttaat tgcgctgctg cgtacaccga ttatcgatct ggcactgcat attgttggcg
     6061 gtagtgaagc agtcctggaa caggcgcggc gctttcttga aatccgctgg ttaagcgcac
     6121 cggcgtcact ggcgaatctg gtattacttg gttggttact cggcgtgcaa tatgcccgtg
     6181 cgccagtaat tttgttagtg gtcggcaata tcctcaacat tgtgctggat gtctggctgg
     6241 tgatggggct gcatatgaac gtgcagggcg cggcgctggc gacggttatt gcggaatatg
     6301 caacattgct gattggtctg ctaatggtgc gtaaaatcct caaactacgc ggaatttccg
     6361 gcgaaatgct gaaaactgcc tggcgaggaa acttccgtcg cttgctggcg cttaaccgcg
     6421 atatcatgct gcgttcgctg ttgttgcaac tctgtttcgg cgcgatcacc gtacttggtg
     6481 cgcgactggg gagtgacatt atcgctgtta acgcggttct gatgacgcta ctcaccttta
     6541 ccgcctatgc gctggatggt tttgcctacg cggttgaagc gcattccggt caggcgtacg
     6601 gtgcgcgcga cggtagccag ttgctggatg tctggcgggc agcgtgccgc cagtcgggga
     6661 tcgtagcgtt actgttttcg gtagtttatt tgctggcagg ggaacacatc attgcgttgc
     6721 tgacgtcgtt aacccagatt cagcagctgg ccgaccgcta tcttatctgg caggtgattt
     6781 tgccgttggt tggcgtctgg tgttatctgc tggacggcat gtttataggc gcaacgcgcg
     6841 cagccgaaat gcgtaacagt atggcggtgg ccgccgcagg ttttgcgctg acgctcctta
     6901 cgctgccgtg gctgggtaat catgctttgt ggctggcgtt aaccgtcttt ctggcgttgc
     6961 gcgggctttc tctggcggct atctggcggc gtcactggcg caacggtacc tggtttgccg
     7021 caacgtgacg gttaaaaatt ctgaataaat aatcctaagc caaattgctg actacactta
     7081 atctcacgtt cagaagaaaa gtgaacgtac tctcattcac aacctaacga tgaggtcttg
     7141 attatgaata aagatgaagc cggcggtaac tggaaacagt ttaaaggtaa agtgaaagag
     7201 caatggggca aactgaccga tgatgatatg acgatcattg aaggtaaacg tgatcaactg
     7261 gtcggtaaaa tccaggaacg ttatggttat cagaaagatc aggcagaaaa agaggtcgtg
     7321 gattgggaaa cccgcaatga atatcgctgg taattaatcc ctcctgcccg acgtgtacaa
     7381 ggatgtacgc cctcttaacg cggtttcttt ttcacctgca cagagtgatc atgctggcac
     7441 tgttcaggat gacgacacgc ttccacttcc acgcatgccg cacagagccc atgtgcttca
     7501 atcacattat gccgcagggc aaaccccatt tttgccgcca gcgtatgcat gatgtcttcc
     7561 acgccttctg cacactcttc tttcactgcg ccgcagcgat cgcaaataaa catggctgaa
     7621 gtatgggtgg actggtcgaa cagatgacag agcacataac tgttggtgga ttccacctta
     7681 tgcacaaaac cttgctcaag cagaaaatcc agcgcgcgat aaaccgttgg cggcttggct
     7741 tgcggttcag cttcgcgcag taaatcaagc agatcataag cgctgatagc gccatcctgc
     7801 agactcatca ggcgcaacac ttccaggcgc tgtggggtca ggcgcacatt acgctgcgcg
     7861 cagatttttt cagcctgcgc taataactcc tgcgttgtgg tcttttccat cgggcacctc
     7921 aaagtggggg ttaagaatgt ccttacttta ccatgttcca ggaaaaacac cgagaaccct
     7981 tattgttgcc gtaatgttga ttttctgttt tgtaggtaag gtgttatgtt gccttgtcgt
     8041 accattatca acacgataat aattaataat tcatttttaa ataaattgct tcaagttaac
     8101 tcgctattta tttaactggg tttaaacgca tacctgtagt tcagaaatta tgagcgattt
     8161 ttgatagtga ccagatggca aaagaattat aaggaggaca cggtacgaca aaaaaggtag
     8221 atgaatcatg ttgaagataa tacccggtgc aactggatac tttaataaaa cactcaattc
     8281 aaatcaattc gataatgaag atgcaatcaa agataaatta gataatagag gttcaatcaa
     8341 aggtaaatta aataatatat atggtaaatc tattgattat gcagcgctgc gtcatcggga
     8401 tataattatt gcaaaaatag atttgtttat tcaacgaatc acgcataatt tatggcatgc
     8461 acgtaaaaaa atgtgttttt aattgagcaa ataaatgatc tcaaaatgtg ggtcaataag
     8521 tatattgacg attgcactga tgaggattta aacgatcgtg actttattgc atcagttgtt
     8581 gaccgggcta tttttcattt cgcgattaat agtatatgta a
//
LOCUS       DAJTMC010000172         8551 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.172, whole genome shotgun sequence.
ACCESSION   DAJTMC010000172 DAJTMC010000000
VERSION     DAJTMC010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8551
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.172"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            97..972
                     /locus_tag="OO130_003532"
     CDS             97..972
                     /locus_tag="OO130_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HCR5366797.1"
                     /translation="MFNRGRNKADKVDIDLSHSNLAPGRLCADGQYRQIVTVEDAVRG
                     ACNLFDLDQLRMEYSPDEYQNLLMCEFVDDLASVFPLSELQACMVDSWEVWTDFHALA
                     LRPFGWREVWIGYDPAKGTQNGDSAGCVVVAPPAVPGGKFRILERHQWRGMDFRAQAD
                     AIKKLTEQYNVTYIGIDSTGVGHGVYENVKAFFPAVREFVYNPNVKNALVLKAYDIIS
                     HRRLEFDAGHTDIAQSFMAIRRATTASGNRPTYEASRSEEASHADLAWATMHALFNEP
                     LQGESANTSNIVEIF"
     gene            972..1568
                     /locus_tag="OO130_003533"
     CDS             972..1568
                     /locus_tag="OO130_003533"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5366798.1"
                     /translation="MGKSKKNRAAATKQTQHKNQTSAEAFSFGDPVPVLDRRELLDYV
                     ECVQTDRWYEPPVSFDGLARTFRAAVHHSSPIAVKCNILISTYIPHPLLSQQAFSRFV
                     QDYLVFGNAYLEKRTNRFGEVIALEPALAKYTRRGLDLDTYWFVQYGMTTQPYQFTKG
                     SIFHLMEPDINQEIYGLPGYLSAIPSALLNESATLFRR"
     gene            1602..2003
                     /locus_tag="OO130_003534"
     CDS             1602..2003
                     /locus_tag="OO130_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCR5366799.1"
                     /translation="MYMTDAAQNQEDVNNLRNAMKSAKGPGNFRNLFMYSPNGKKDGL
                     QIIPLSEVAAKDEFLNIKNVSRDDMMAAHRVPPQMMGIMPNNVGGFGDVEKASLEFVR
                     NELIPLQKRMMEINKLVSENIVKFDSYKLAT"
     gene            complement(2110..2991)
                     /locus_tag="OO130_003535"
     CDS             complement(2110..2991)
                     /locus_tag="OO130_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004982020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5366800.1"
                     /translation="MKWAIEMNKLKRPVFKDYSAFKKLSANKRLKHHSVLNGQDTFIL
                     NAYRSYIKNKGYLNVNSCGVLSNNIAEALRYYYNNPAKCLSIIDDIRAANSNSLCPMC
                     GSMHSGTLDHVLPKENYPEFSLFTKNLVPACKCNTFKSTTIANSAGHRMLHPYFDNIL
                     KQRLICANFSKLGRTPTIDVKIIIDPMSPEYNNVKYHLDNVVIKNNIKGYLSEQWESF
                     YLYPELVIRGLNKSLSTYSDVYELLTRELLLLDEKHKGKNNWNSVFVAGLIRPDVIRW
                     ILCELHLGNRSPDGRLI"
     gene            complement(2975..4624)
                     /locus_tag="OO130_003536"
     CDS             complement(2975..4624)
                     /locus_tag="OO130_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000960487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR5366801.1"
                     /translation="MLVKKNKNYSLYFLITNNQQISDSGNYIKIVPSRSDWNDFGYLS
                     RVEISIFNKKECKDIISLNGYIGFLNSEEERNGKAELIRRVKKKEESNLKDEGFNDYF
                     VMLQDMNNYRRLVRFFGVNESKYILNFINDVVSKKSDASAENLRKKAISSDIFNKSFI
                     RESESYFTFKNAGTVLSGIQYEELGNLSQHIKISYSNKVNEEDVSYVFNFDHEHDLPK
                     RISIIIGENGVGKSQTLREIALAAIKGKDNLVAVVDSNGVSIEERIQISRLIAFSPTN
                     ESKCSFPTDKRIKSLIWYKRLTLNRESKSKLNLNDLIVQLARSNDAIGYNQRWNIFVD
                     ALSVLNSNNDLVIKCGGENSPFVKLNELNSRGEESRLSLYSEIILSGEPLRLIENKCY
                     PLSSGEISFIKFCVQVCLYIENGTLLLIDEPETHLHPSFINKFMALLDNLLEKTGSSA
                     IIATHSVYLVREVFKEQVTILRRRDDGVIVSEKPRLSTFGANIGNISYFVFGESEPTQ
                     ILNKVKNNIIAKNMSWDDVESKYSEDLSLNVLTALRNEMGN"
     gene            complement(4917..5150)
                     /locus_tag="OO130_003537"
     CDS             complement(4917..5150)
                     /locus_tag="OO130_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI family protein"
                     /protein_id="HCR5366802.1"
                     /translation="MRIEIMIDKEQKISQSTLDALESELYRNLRPLYPKTVIRIRKGS
                     SNGVELTGLQLDEERKQVMKIMQKVWEDDSWLH"
     gene            complement(5161..5349)
                     /locus_tag="OO130_003538"
     CDS             complement(5161..5349)
                     /locus_tag="OO130_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5366803.1"
                     /translation="MQDYFLESLKLQRIDFFLKLVAASECSDEEKGLALQWVSELTDE
                     LMAKIRTHEYNRSMDVIS"
     gene            complement(5503..7917)
                     /locus_tag="OO130_003539"
     CDS             complement(5503..7917)
                     /locus_tag="OO130_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HCR5366804.1"
                     /translation="MSHADMNNCCGFNEAAAAFSWNSPKKAINPYLDPAEVAPVSALS
                     NLITLYAADNEQEQLRREALSDQVWERYFFYESRDPVQHEMEQDKLISRAKLAHEQQR
                     FNPDMVILADVNAQPSHISKPLMQRIEYFSSLGRPKAYSRYLRETIKPCLERLEHVRD
                     SQLSASFRFMASHVGLDGLLILPEMSQDQVKRLSTLVAAHMSMCLDAACGDLYATDDV
                     KPEEIRKTWEKVAAETLRLDVIPPAFEQLRRKRNRRKPVPYELIPGSLARMLCADWWY
                     RKLWKMRCEWREEQLRAVCLVSKKASPYVSYEAVMHKREQRRKSLEFFRSHELVNEEG
                     DTLDMEDVVNASSSNPAHRRNEMMACVKGLELIAEMRGDCAVFYTITCPSRFHSTLNN
                     GRPNPTWTNATVRQSSDYLVGMFAAFRKAMHKAGLRWYGVRVAEPHHDGTVHWHLLCF
                     MRKKDRRAITALLRKFAIREDREELGNNTGPRFKSELINPRKGTPTSYIAKYISKNID
                     GRGLAGEISKETGKSLRDNAEYVNPWASLHRVQQFRFFGIPGRQAYRELRLLAGQAAR
                     QQGDKKAGAPVLDNPRLDAILAAADAGCFATYIMRQGGVLVPRKYHLIRTAYEINEEP
                     TAYGDHGIRIYGIWSPIAEGKICTHAVKWKMVRKAVDVQEAAADQGACAPWTRGNNCL
                     LAENLNQQEKDKSADGNPRTDITSMDDKELHDYLHSMSKKERRELAARLRLVKPKRRK
                     DYKQRITDHQRLQLVYELKSRGFDGSEKEVDLLLRGGSIPSGAGLRIFYRNQRLQEDD
                     QWRNLY"
     gene            complement(7914..>8551)
                     /locus_tag="OO130_003540"
     CDS             complement(7914..>8551)
                     /locus_tag="OO130_003540"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Dam family site-specific
                     DNA-(adenine-N6)-methyltransferase"
                     /protein_id="HCR5366805.1"
                     /translation="RVLFEIANREVAYYNIRQEFNYSTEITDFMKAVYFLYLNRHGYR
                     GLCRYNKSGHFNIPYGNYKNPYFPEKEIRAFAEKAQRATFICASFDETLAMLKAGDVV
                     YCDPPYDGTFSGYHTDGFTEDDQYHLASVLEHRSSEGHPVIVSNSDTSLIRSLYRNFT
                     HHYIKVKRSIGVAAGEGKSATEIIAVSGPRCWVGFDYSRGVDSSAVYGVRA"
BASE COUNT         2310 a         2120 c         1766 g         2355 t
ORIGIN      
        1 accttaaaat caccatcata cactaaatca gtaagttggc agcattaccc accatccagt
       61 ctgacccaca gtgcttatcc gttctggtcc ggtgcgctgt tcaaccgtgg gcgcaacaaa
      121 gctgacaagg tggacatcga cctgtcccac agcaatctgg cccccggccg gctgtgcgca
      181 gacgggcaat accgccagat agtcaccgtg gaagatgcgg tgcgcggcgc ctgtaacctg
      241 ttcgaccttg accagttgcg catggagtac agccctgacg aataccagaa cctgctgatg
      301 tgcgagttcg tggacgatct cgcgtccgtg ttcccgctca gcgaattgca ggcgtgcatg
      361 gtggacagct gggaagtctg gaccgacttt catgcactgg ccctgcgccc gtttggctgg
      421 cgcgaagtgt ggatcggtta tgacccggca aaaggtacgc agaacggcga cagcgccgga
      481 tgcgtggtgg tggcaccgcc agccgtgcca ggcggtaagt tccgcattct tgagcgtcac
      541 cagtggcgcg gaatggactt tcgcgcccag gctgacgcca tcaaaaaact gactgaacag
      601 tacaacgtga cctatatcgg catcgactca accggcgttg gtcacggggt ttacgagaac
      661 gtgaaagcgt tttttcctgc cgtccgggag tttgtctaca accccaacgt taaaaacgcc
      721 ctggtactca aggcctacga cattatcagc caccgccgtc ttgagtttga cgccgggcac
      781 accgacattg cgcagtcatt catggcaatc cgtcgcgcca ccaccgccag cggcaaccgc
      841 ccgacctatg aagccagccg cagcgaagaa gccagccatg ccgatctggc ctgggcaaca
      901 atgcacgcac tgtttaacga accgctgcag ggcgagtccg ccaataccag caatattgtg
      961 gagatttttt gatgggaaag agtaagaaga accgcgctgc ggcgacgaaa cagacccagc
     1021 ataaaaacca gacttcagcc gaagcattca gcttcggcga tcccgttccg gttctggacc
     1081 gccgtgaact actggactat gtggaatgcg tacagacaga tcgctggtat gagccgccag
     1141 tgagttttga cggactggcg cgcaccttcc gcgccgccgt gcatcacagt tcaccaattg
     1201 cggtgaaatg caacattctg atcagtacct acatccctca cccgctgctc agccagcagg
     1261 ctttttcacg ttttgtgcag gattatctgg tatttggtaa cgcttacctg gagaaacgca
     1321 caaaccggtt cggagaggtc atcgctcttg agcctgctct ggcaaaatac acccgacgcg
     1381 gattagacct agatacctac tggtttgtgc aatacggcat gaccacgcag ccgtatcagt
     1441 tcacgaaagg aagcatcttt catctgatgg aaccggacat caaccaagag atctacggcc
     1501 tgccaggtta cctttctgcc attccgtcag ccctgctcaa cgagtccgcc acgctgttcc
     1561 gccgatagta ttacattaac ggcagtcatg caggcttcat catgtacatg accgatgccg
     1621 cgcaaaacca ggaggatgtg aacaacctcc gcaatgcgat gaaaagcgcc aaaggaccag
     1681 gtaacttccg caatctgttt atgtactcgc ctaacggcaa aaaggacggg cttcagatca
     1741 tcccgttgtc agaagtcgcg gcgaaggatg agtttctgaa catcaagaac gtgagccggg
     1801 atgacatgat ggcggcgcat cgtgtgccac cgcaaatgat ggggattatg cctaataatg
     1861 tcggggggtt tggggatgtg gagaaggcga gcctggagtt tgtacgtaac gagttaatac
     1921 cgttacaaaa aagaatgatg gaaataaata aattggtaag tgagaacatt gttaaatttg
     1981 atagttataa actagctact taacgtaatc actaaatgag gttctttaac acagctaaaa
     2041 aatttattaa tagccagcag tttttatgaa tttcttcagc gtgcagatac acgctgaaga
     2101 tgcaaaaaat catatgagac gaccgtctgg acttctgtta cctaaatgta attcacagag
     2161 tatccaccta atcacatcag gtcgaattaa cccagctaca aaaaccgaat tccaattatt
     2221 tttaccttta tgtttttcat ccaacagcaa caactcgcga gtcaacaact catatacatc
     2281 gctataagta gaaagactct tattcagtcc tctaataaca agctcaggat ataagtaaaa
     2341 cgactcccat tgttcagaca aatacccctt aatattattc ttgataacaa cattatcaag
     2401 atgatactta acattgttat attctggtga cattgggtca attatgattt ttacatcaat
     2461 tgtaggtgtt cgccctaatt tagaaaaatt agcacatatt aatctttgtt ttaatatatt
     2521 atcaaaatat ggatgcagca tccgatggcc tgcagaatta gctatagtag tacttttgaa
     2581 agtattacat ttgcaagcag gaaccaaatt cttagtgaat aaagaaaatt ctggatagtt
     2641 ttccttgggt aaaacatgat ccaaagtccc gctatgcatt gaaccacaca ttggacatag
     2701 agagttagaa ttagctgctc ttatatcatc aattatagat aaacactttg cgggattgtt
     2761 ataataatat ctcaaagctt cagcaatatt gttactcaat acgccacaag aattgacatt
     2821 aagataccct ttattcttta tgtacgatct atatgcatta agtataaatg tatcctgtcc
     2881 attcagaaca gaatgatgtt ttaatctctt atttgcagat agttttttaa atgcagaata
     2941 atctttaaaa acaggtcttt ttaatttatt catttcaatt gcccatttca tttctcaacg
     3001 cagttaatac attaagtgat aagtcctcgc tatatttaga ttcaacatca tcccaagaca
     3061 tattctttgc aattatattg ttttttactt tatttaaaat ttgagttggt tcactctcac
     3121 cgaaaacaaa atatgaaata tttcctatat tcgccccaaa agtactcaat ctcggttttt
     3181 cgctaacaat taccccatca tctcgtcttc tgagtattgt cacttgctcc ttaaaaacct
     3241 cccgaacaag ataaaccgaa tgcgttgcta tgatagctga agagcctgtc ttttctaata
     3301 aattatcaag caatgccata aatttattaa taaatgaagg atggagatgt gtttctggct
     3361 catctattaa tagaagagtc ccattctcaa tatacaaaca gacttgtaca caaaacttaa
     3421 taaacgatat ttccccacta ctcaaagggt aacatttatt ttcaataagc ctcaaaggct
     3481 cgccagagag aataatttca gaatataatg atagtctaga ttcttcgcct cgagaattaa
     3541 gctcatttaa tttaacaaat ggtgaatttt caccgccaca tttgattact aaatcgttgt
     3601 tactatttag aacactcagt gcatcaacaa atatattcca cctttgatta tagccgatag
     3661 cgtcgttact cctcgctagt tgcactatta aatcattcaa gtttaatttg gattttgatt
     3721 cacgatttaa agtcaatctt ttgtaccata tcaatgattt tattctttta tcggttggaa
     3781 atgaacactt actttcattt gttggagaaa aagcaatgag cctactaatt tgaattcgtt
     3841 cttcaatgct cacaccgtta gaatcaacta cagccaccaa attatccttt cctttaatcg
     3901 cagccaatgc gatttctcta agcgtttggc tttttcctac accattctcg cctataatta
     3961 tggaaattct ttttggtaaa tcatgctcgt gatcaaaatt aaaaacataa gatacatctt
     4021 cttcattaac tttattcgaa tagctaatct tgatgtgctg tgataaatta cccaactcct
     4081 catattgaat accggaaaga actgttcctg catttttgaa agtaaaatac gactcagact
     4141 ctctaatgaa agatttatta aagatatcag aagagattgc ttttttcctc aaattctctg
     4201 cactagcgtc acttttctta gaaacaacat cattaatgaa atttaatata tatttggatt
     4261 cattcacccc aaaaaatctt actagtcttc gataattatt catatcctga agcatgacaa
     4321 aataatcatt aaacccctca tcctttaaat tagattcttc ctttttcttc actcgcctta
     4381 tcagttctgc ttttccattt cgttcctcct ctgaattcaa aaaaccaata taaccattca
     4441 gtgaaattat atccttacac tcttttttat taaaaataga tatttcgact ctactcaaat
     4501 atccaaaatc attccaatcg cttcttgatg ggactatttt tatataatta ccagaatcag
     4561 atatctgttg attatttgta attaaaaaat ataaactata atttttattt ttcttgacta
     4621 acattattac cctctcactt cattagtgat gatttatttt ggaattattt tacacactta
     4681 tacctagcgc gcaatcgtat ccccgccacg cctgcccgct ttatgtagag gttttcatgc
     4741 acctgcatga tctacgcaaa agcccgccag ttctggcggg ccttagcaaa aacgatcctc
     4801 aaacgatcat gcgatctcat gcggcataga catgcactac agagctaacg cctcgcaagg
     4861 gctcgttgtt caaccttgct gacgccagaa gctagttcag acgccagcaa cgtttcttaa
     4921 tgcagccagc tgtcgtcttc ccacaccttc tgcataattt tcatcacttg ttttctttct
     4981 tcgtccagtt gcaatccagt aagttccaca ccgttagagc tacctttgcg gatacgaatt
     5041 accgttttgg gatacagagg gcgcagattg cggtaaagct cggattcaag agcgtccagg
     5101 gtagactggc taatcttctg ctctttatcg atcattattt caatgcgcat aaaagtcacc
     5161 tcagctgatg acatccattg agcggttgta ttcgtgggtt ctgatttttg ccatgagttc
     5221 atcagtcaat tcagaaaccc actgcagagc cagccccttc tcttcatcac tacactcact
     5281 agccgctaca agcttaagaa aaaaatcaat gcgctggagc ttcaaagact ccaaaaaata
     5341 gtcctgcatc tttcctccta tgacaccaca cgcaatactg tatgcataac cactgtttat
     5401 atttacagca tataataatc ttactgatgt aaaacgtttt tttacgttca tcagcctgat
     5461 atgcctggta ttattaagag cacgaattgt taacccgcgt aattaataca ggttccgcca
     5521 ctgatcatct tcctgcaaac gctggttccg atagaagata cgcaggcctg ctcctgacgg
     5581 aatactgcct ccgcgaagga gtaaatcgac ctctttctcg ctgccatcaa atcctctgga
     5641 cttcagctca tacacgagct gcagtcgctg atggtctgta attcgctgtt tgtagtcttt
     5701 acgccgtttc ggtttcacca ggcgtaacct tgctgccagt tcccggcgct cttttttgct
     5761 catactgtgc aggtaatcgt gcaactcctt gtcatccatg ctggtaatgt ccgttctggg
     5821 gttcccatca gctgatttat ctttctcctg ttggttcaaa ttttcagcaa ggagacagtt
     5881 attgccacga gtccaagggg cgcaagcgcc ctggtcggct gccgcctcct gaacgtcaac
     5941 ggctttacga accattttcc acttcactgc atgagtgcag atcttgcctt ctgcaatggg
     6001 tgaccagatg ccataaatac gaataccgtg atcgccatag gcggtcggct cttcattgat
     6061 ttcataagcg gttctgatca agtgatattt gcgaggaacc agcacgccgc cctgcctcat
     6121 aatgtaggtg gcaaaacagc cagcatcagc agcagccagg atggcatcaa ggcgcgggtt
     6181 atccagtacc ggcgcacctg cttttttgtc accctgctgc cttgccgcct gaccagccaa
     6241 caaccgcagt tcacggtaag cctgacgccc tggaatacca aagaagcgga attgctgaac
     6301 acgatgcaga gacgcccagg gattaacgta ttcagcgtta tcacgcaggg atttacccgt
     6361 ttccttgctg atctcgccag ccagaccacg cccgtcaatg ttcttactga tgtatttcgc
     6421 gatgtagctt gtcggcgttc ctttgcgcgg gtttatcagc tcagatttaa agcgtggacc
     6481 agtgttatta cccagttcct cgcggtcttc acggatggca aacttacgca acaatgcagt
     6541 aatggcgcgg cggtcttttt tgcgcataaa acacaacagg tgccagtgaa ctgtaccgtc
     6601 atgatgcggc tcagccaccc gcacgccata ccagcgcaac ccggctttgt gcatcgcctt
     6661 acgaaatgca gcaaacatgc cgaccagata atcgctgctt tgtcttaccg tcgcatttgt
     6721 ccaggttggg tttggtctgc cgttattgag cgtggaatgg aaacgtgacg gacaggtaat
     6781 ggtgtagaaa acggcgcagt caccgcgcat ttccgcgata agctccagac ctttaacaca
     6841 ggccatcatc tcattgcggc ggtgcgccgg gttgctgctg ctggcgttta ccacgtcttc
     6901 catatccagc gtgtcgccct cttcgttcac cagttcatga gaacggaaaa actccaatga
     6961 cttgcggcgc tgctcacgtt tatgcatcac ggcttcatag ctgacatagg gagatgcttt
     7021 tttgctgacc aggcagacag cacgcaactg ctcttcccgc cattcgcaac gcattttcca
     7081 taatttccgg taccaccagt cggcgcacaa catacgcgcc agcgaacccg gaatgagttc
     7141 atagggcacg ggtttacggc ggtttctttt ccgacggagt tgctcaaacg caggcgggat
     7201 gacatccaga cgcagggttt ccgctgccac cttttcccat gtcttgcgga tttcttctgg
     7261 cttaacgtca tcggtggcat acaaatcacc acaagctgca tcaaggcaca tgctcatatg
     7321 cgcagcgaca agggtagaca ggcgtttcac ctgatcctga ctcatttcag gcagaatcag
     7381 caggccgtcc agccctacat ggcttgccat aaaacgaaaa gatgcagata gctggctgtc
     7441 gcgtacatgc tccagtcgtt ccagacatgg cttaatcgtc tcacgtaaat agcgggaata
     7501 agcctttggc ctgcccaggc tgctgaagta ttcaatacgt tgcatcagcg gcttgctgat
     7561 atgggaaggc tgggcgttga cgtccgccag aatgaccata tccggattaa aacgctgctg
     7621 ctcatgcgcc agctttgccc ggctaatgag cttatcctgc tccatttcgt gctggacagg
     7681 atcacgtgat tcataaaaaa aataacgctc ccagacctga tcactcagtg cctcgcggcg
     7741 caactgttcc tgctcgttat cggcagcgta cagagtgatc aggtttgaaa gcgcagaaac
     7801 cggcgcaact tccgccgggt ccagataagg gttaatggcc tttttcgggc tgttccatga
     7861 gaacgctgcg gcagcctcgt taaagccgca gcagttgttc atatcggcat ggctcatgca
     7921 cgtactccgt acacggcaga actatccacg ccacgcgaat aatcaaatcc cacccagcag
     7981 cgcggcccgg aaacagcaat gatttctgtt gctgatttac cctcgccagc tgccacaccg
     8041 atgctgcgtt ttaccttgat atagtggtga gtaaaattgc gatacagcga acggatcagg
     8101 gatgtgtcac tgttagaaac aatgaccgga tgtccttctg atgaccgatg ttcaagaacg
     8161 gatgctaggt gatactggtc atcttcagtg aaaccatcag tgtgatagcc ggaaaacgta
     8221 ccgtcatacg gtggatcgca atacaccaca tcccccgcct tcaacatcgc cagcgtttca
     8281 tcaaagctgg cgcagataaa cgttgctcgc tgggcttttt ctgcaaatgc gcgaatttct
     8341 ttttcaggga aatacggatt tttataattg ccgtagggaa tgttgaaatg cccgctcttg
     8401 ttatagcgac ataaaccacg gtaaccgtga cgattgagat acaggaaata taccgctttc
     8461 atgaaatcag taatttcagt tgagtaatta aactcctgcc ttatgttgta ataagccacc
     8521 tccctgtttg cgatctcaaa taaaactctg g
//
LOCUS       DAJTMC010000173         8452 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.173, whole genome shotgun sequence.
ACCESSION   DAJTMC010000173 DAJTMC010000000
VERSION     DAJTMC010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8452
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.173"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(141..2684)
                     /gene="mdoH"
                     /locus_tag="OO130_003541"
     CDS             complement(141..2684)
                     /gene="mdoH"
                     /locus_tag="OO130_003541"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HCR5366806.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKHWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(2677..4212)
                     /gene="mdoG"
                     /locus_tag="OO130_003542"
     CDS             complement(2677..4212)
                     /gene="mdoG"
                     /locus_tag="OO130_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HCR5366807.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGGAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            4606..5763
                     /gene="mdoC"
                     /locus_tag="OO130_003543"
     CDS             4606..5763
                     /gene="mdoC"
                     /locus_tag="OO130_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HCR5366808.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMLVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(5771..7252)
                     /gene="clsC"
                     /locus_tag="OO130_003544"
     CDS             complement(5771..7252)
                     /gene="clsC"
                     /locus_tag="OO130_003544"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HCR5366809.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLVAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRQMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(7194..7727)
                     /gene="ymdB"
                     /locus_tag="OO130_003545"
     CDS             complement(7194..7727)
                     /gene="ymdB"
                     /locus_tag="OO130_003545"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HCR5366810.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(7823..8137)
                     /locus_tag="OO130_003546"
     CDS             complement(7823..8137)
                     /locus_tag="OO130_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000901553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5366811.1"
                     /translation="MNVQTFFKLSYARQFFFPFIAIAGSTAQGGVIHFYGQIVEPACD
                     VSTQSSPVEMNCPQNGSVPGKTYSSKALMSGNVKNAQVASVKVQYIDKQKKLAVMNIE
                     YN"
     gene            complement(8254..>8452)
                     /gene="csgC"
                     /locus_tag="OO130_003547"
     CDS             complement(8254..>8452)
                     /gene="csgC"
                     /locus_tag="OO130_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706954.2"
                     /note="involved in autoagglutination of curliated cells;
                     not involved in production of curli fibers; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HCR5366812.1"
                     /translation="LREGGSGQSQTKQEKTLSLPANQPIALTKLSLNISPEDRVKIVV
                     TVSDGQALHLSQQWPPSSEKS"
BASE COUNT         2105 a         2210 c         2003 g         2134 t
ORIGIN      
        1 aacgccggta tttttattgg ctcgttgttt ttgccggatg cggcgtgaac gccttaccca
       61 acctacaaaa tcttgcaaaa tcaataaatt gcaggaacga tgtaggcctg ataagcgtag
      121 cgcatcaggc aactacgttt ttattgcgaa gccgcatccg gtttacgcaa tgccagtgga
      181 ttgagcttta tcccttcgta ataactcacc catgaagaat atctctccgg ggaattccag
      241 acacggaaat gcagacgggc catcgtcacc ggatcgctta gcagcaccag gcgacgatcg
      301 cgattcagct tctctggcgt ctcgttaagc gcctgttcaa cgtggcggtc acgggcgatt
      361 tccagcacct tactggcgcg gtgacgcgcg gtcgccattg cggttgccag agcgttaaat
      421 gacgggttaa acaccgcgtg cataaaacca tcatcaaggg agcgttgacg attcatctca
      481 aggaagcgat cggtatcaac cagcacctgc ggcggtgaat actcttccgg gatcaggaac
      541 agtttccagt gtttggttcg cagaccaacg gtggcacggc tggaaatcac cgaaacaaac
      601 ggtgacagga tcaacgagaa gacaatcggt gccagccaga acaggaaacg cagatccagc
      661 cacgccatcc caacagccca cactaaccct aacagcagct gtgagccgtg gcgtttgaac
      721 gcttcacccc aggaagtgga gtcatcatca cgctgcggtg aattccacac cacttcccag
      781 ccaaggaacg cgctgacaac gaagaccgta tggaacagca tgcgtaccgg agccagcagc
      841 acggaaaaaa gcacttccag tagcaacgat aatgtaacgc gccagaagcc gccgtattct
      901 ttcgttcctt tgcaccagat aagcaaaatg ctcaataact tcggcaggaa caacagcacc
      961 atagtcgaag caaaaagtgc aatcgccagc tcaggacgcc actgcggcca caccgggaac
     1021 aactgccgtg gttgcaggaa gtattgcggt tcggtcaacg catgtactac ctgcaatgca
     1081 gtagagagcg cgaggaacat aaaccacagc ggagcggaga gataagacat cacgcccgtc
     1141 aggaacaccg cacggtgaac cgggtgcata cccttcacca ggaacagacg gaagttcatc
     1201 aggttaccgt ggcaccagcg gcggtcacgt tttagctcat caagcaagtt aggcggtaat
     1261 tcttcataag aacccgggag atcgtaagca atccagaccc cccaacctgc acggcgcatc
     1321 aacgccgctt ctacaaagtc atgtgacagg attgaaccgg caaaggaacc ttcgcccggc
     1381 agcggagcca gtgcgcagtg ctcgataaac ggtttcacgc ggataatcgc gttatgtccc
     1441 cagtagtgcg actcgccaag ttgccagaag tgcaaaccgg ctgtaaacag tggcccatac
     1501 acgcgggtcg cgaactgctg acagcgcgca tacagcgtat ccataccgga cgctttcggc
     1561 gacgactgaa tgatcccggc gttcgggttg gcttccatca ggcgcaccag cccgcacaaa
     1621 caatcaccgg tcattaccga gtcagcatcc agcaccacca tgtagctgta ctggctgccc
     1681 cagcgacggc agaagtcatc gatattaccg cttttacgct tcacgcggcg acggcggcgg
     1741 cgatagaaaa tctgaccttc gccaccgact tcagcgataa gctccatcca ggctttttgc
     1801 tctgcgacgc agatatccgg gttataactg tcactaagaa tgtagacatc aaagtgtttg
     1861 gcattcccgg tggcttttac tgattcccac gttgcacgca ggccagcaaa aacacggttc
     1921 acgtcttcgt tacagatagg catgatcaac gccgtgcgat gctccgggtt taatggttca
     1981 tcgccaactg ttgacgcaga tatactgtat ttatcgcgac caataagcag ttgcaggaag
     2041 cccattaacg ccgtccagaa tccggcggac acccaacaga acagtaccgc aaagaggatc
     2101 aggataccgg tttgcagcat ataaggcaga agctgcataa aggaaaccca caaatcctga
     2161 ccaaccatat ccataggatt aatcagcgcc caaccctgat aaggaagaat ggtcttcata
     2221 taccaggtcg cgacgacagt ttgcgcgagc gtcaggatca acagaatgta acggcggatg
     2281 gtaccgacgg tacgccactt ttgctcgctc tcctgctctt ctttggtcaa acgagccaga
     2341 tagcgcggcg tgacatcgcg tccacgcagg cgatcccaga aacggcctac cgggttggta
     2401 cgccacgggt cgggaaacat cgaggagcgt tttgcttctg gcatcgcctt cagctgatcg
     2461 cgcccttcgt cgtctttaat taactgtcca tcagcaagtg aatctggcca ggcttgttcc
     2521 agacgcgcct ttaccgagcc ttgcggggaa tcatcctccc gcgcccaggt gcggtgttcg
     2581 gcatccagcg cctgatgtac ggcgcggata tcagtcttcg gcaatgccgc tttctcgctt
     2641 gcggcgatgg gcattgcgtc aatgtactca gttgtcttat tcattggcag gtaactggta
     2701 gctccaggtt tcactcaacg tctgatcggc attcaccagc gcagcacgca tttcagtggt
     2761 tttcttggca tctttcactt tcacacgcat caccagacgc cagcctttgg taaccgggtt
     2821 atagcgcacc gtgctttcaa ctatctcacc attatcacca atgctggttt gcgctgtgac
     2881 cggggtatcc tctggcagtt ttttcatctc tgcgccggta aaatcgacca caaaggcgat
     2941 agtaccgtca ggctggcgaa tcaggttcga ctgcttcaca tcccccgttg aacgacgcgt
     3001 ttgttgcacc catgcgttat ctggcgcatg cagtttgtct tcatcacggc tgaaggtgat
     3061 ggtgtattta aagttcatct ctttacccgg ctccggcagc tgatccggcg tccagtaagc
     3121 gacgatgtta tcgttggttt catcgttggt tggaatttcc accagctcaa cgctgccttt
     3181 accccactcc cctttcggag tcacccatgc gcttggacga agatcgtaac gatcatcgag
     3241 atcttcaaag cgggagaaat cacgaccgcg ctgcaacaga ccgaagcctt gcgggttctc
     3301 catcgagaag ctgctaaccg ctaaatgttt cgggttattc aacggacgcc agatccattc
     3361 gccattaccg gcatggatag agagaccgtt agagtcgtgc aactccggac gatagttatt
     3421 tgcaggcgac ggttggttcg gcccaaacag gaacatactg gttaacggtg caccccccag
     3481 tttgccgact ttatcgcgca gatagatttt cgactgcaca tccacaaccg tgtcacgccc
     3541 tggcataacc acgaatttgt aagcacctgt cgcgcgcggc gagtcaagca atgcataaat
     3601 ggttaaacgt ttatcagtcg gttttggacg ctcgatccag aactctttga agcgtggaaa
     3661 ttcttcaccc gatggcaagg cggtatcaat tgccaggccg cgggcagaaa ggccataaac
     3721 ctgacctgca ccaatcacgc ggaaatagct ggccccgagc atgctgacga tttcatcgtt
     3781 tttatctttg ctgttgatcg ggtaaagcac tttaaaaccg gcaaaaccaa ggtcttttac
     3841 cgtgtctttg tcatgctgaa catcgccgaa agtgaaataa tccgggctgt atttgattcg
     3901 tttgactgcg gtggcagtca cttcatttat tttgaccggg gtatcgaagt acataccctg
     3961 atggtagaac tcgagtttga atggggtctt cagattgttc cagtacgctt tgtcatgatt
     4021 aaactggatc tgctgatagt ccgcgtattt catatcgcgg aaaacggagg gcaagttgct
     4081 tttgggcgcc tcatagcctt tcccggctaa ggattgagct tgctttgcga catcatcaat
     4141 actgaaagcc cagcttgaag atgtatacag ggttaacatt actgcagcac tcaaccaacg
     4201 cattttcatc atttgtagtt tatgtttcat aattagtaag cacttccccc tttgtgtgct
     4261 tatatcgatc cgatctattt taatggataa ccagccattc cgacaacaga atcccggtat
     4321 cgttcagcac gctggctcat ggattaagca cctgattgca accgttttca ctttccgtgc
     4381 ttctcctaaa gacagaagca tcgacttcgt gtagggtttg ccagcaattt tatttatcaa
     4441 ccgtcttaca gacgcttcca ttctggttag tggtagcgat tatgctatgc ataaaaacct
     4501 tgaagcctgc gcctggcgcg gtcaggtcgg tgtcgctatg gcaacaagcc taaaggcata
     4561 acccgacagg gataagacga aaaatccgag attagttaac catatatgaa tccagtaccc
     4621 gcgcaacgtg aatatttcct cgactccatc cgcgcctggc tgatgttgtt agggatccct
     4681 tttcatattt ctttaatcta ttcgagccat acatggcatg tgaatagcgc cgaaccgtca
     4741 ttgtggctga ccctttttaa tgacttcatc cactcgttcc gcatgctggt atttttcgtt
     4801 atatccggct acttttccta catgcttttt ttacgctatc ccttgaaaaa atggtggaaa
     4861 gtacgtgtcg aacgtgtagg tatcccgatg ttaacagcca tccccttact gacattgccg
     4921 caatttatta tgctgcaata cgtcaaaggg aaagcggaaa gttggcctgg gctgtcattg
     4981 tatgacaaat ataatacgtt ggcctgggaa ttaatatcac acctgtggtt tttactggtg
     5041 ttagtagtca tgacgacgct gtgcgtatgg atatttaaac gcatcagaaa taatttagaa
     5101 aattctgata aaacgaataa aaaattctcg atggtaaaac tatcggtgat ttttttatgc
     5161 ctcggcatcg gctatgcggt aataagaaga acgattttta ttgtgtatcc gcccattctg
     5221 agtaatggca tgttcaattt tattgtcatg caaacgctat tttatttgcc gttctttatc
     5281 ctcggtgcac tggctttcat tttccctcat cttaaagcct tgtttaccac gccgtctcgt
     5341 ggctgtaccc ttgcagcagc attggcattt gtcgcttatt tactcaacca acgctatggc
     5401 agtggcgatg cctggatgta cgaaaccgag tcggtgatca ccatggtcct cggtctgtgg
     5461 atggtgaatg tggtcttctc ctttggccac cgtttgctta acttccagtc agcgcgggtg
     5521 acttactttg ttaacgcatc gctatttatc tatctggttc accacccgtt aacgctgttt
     5581 ttcggcgcat acattacacc gcacatcacc tccaactggc ttggttttct ctgtggcctg
     5641 atattcgtag tagggattgc gataattctg tatgaaattc atttacgcat cccgttactg
     5701 aagtttttgt tttctggtaa accggttgtt aagcgtgaga acgataaagc accagcccgt
     5761 taagccacat ttacaataac cattccacgg gcaatatcga cgccagtctg accataaccc
     5821 gcttccagaa actggtggcg ggttcttttt tgagaataat ctctttcttt gcatgacgat
     5881 caacccagtt gatccgtccc cacctgtcca gacggagctg ccaggccgca tcatactggc
     5941 tctgtataaa gcgtttatca attaactgtg ccagcgtttc gctctctatc acgaagccca
     6001 tttcagtatt gagcaatgtc gaacgtggat cgaaattgaa agagccgata aacaccgttt
     6061 taccatcgat gctaaaggtt ttagcatgca ggctggcacc ggaattaccg gttatgccgc
     6121 gatcgtgtaa cgtactactt tgttcacgcg tcggcttgag ttcatataat tccacgccat
     6181 agcggagcaa ttttttgcgc cagcgcgcgt atccggcatg gacgacagca acatcgttag
     6241 cggcaagaga attggttagg atcgcaatct ttaccccttt tctcaccatc cgtaagagtt
     6301 gcgccacacc tgcgcgtgtc ggtacaaaat aggaagagat aatatcgatg cgttcactgg
     6361 gtgagcccat gatatcgaac aggcgctgcg gtaacagtga atgccgtttt gccttgccct
     6421 cccctttcgc cggatcatca cttaataaac gtgttttcgc ccagataagc ggcaacgttc
     6481 catcaaccag atgatttata aacggactgg attccatttg gcgtaagtaa cgatgcgtca
     6541 tggcatcgtt atgccaggag gcgggtaact cgatgcgatc cgccatttca ccttccggga
     6601 catccagcac ctgctgtaag ggtgaaaccg atttgcaata ccagtagcgg gcgaaatcat
     6661 cggcaacgtc ctctaccacg ggtcctattg ccataacatc taaatccgaa aaaagtggct
     6721 cttcccctgc cccaaaatag gcatcaccaa tatttcgtcc tcccaccaag gtaaccacgc
     6781 catcgacagt gaaactttta ttgtgcatac ggcgattgag acgggaaaag tcggtgatat
     6841 aaccaagcgg acgcagcaag cgaaacgaga aaggattaaa aagccggact tcaatgcggg
     6901 gatggctgtc aagcaagcgt aaaatatcgt caagtccggg cgtattgttg tcgtccagca
     6961 acaaacggac gcgaacgcca cgctttgctg cgactaacag ggcggaaaac agtaaccgac
     7021 ccgacatatc gtcctgccag atgtaatact gaacatccag cgtatgctct gccatttcgg
     7081 ccagacgata ccgggcggca aacgcatcca gacttttctc cagagggaaa agaccacact
     7141 gaccgggatg ttgcgaacac agtggcagca ccgcgctcgc cagccggggc aaatcattca
     7201 tctccttgtt gggtaaggag tctttcgtag aggtgggcgt tttcttcatc ataacagaca
     7261 aagtatacct gttcgggtaa agcgtgacgg gtaataaatt ctgaaacggt ttttactgcg
     7321 atttcagccg ctgccgcacg agggtaacca taaaccccag tactgattgc aggaaatgcc
     7381 actgacgtat agctgtttgc cgccaccagt cgcagactat tgagataggc atcctgcaaa
     7441 agctggtctt cgttttgttc accaccacgc cagactggcc cgacggtgtg tactacggct
     7501 ttagcgggaa gatcgcctgc aagcgtaata acggcatgcc ccgtagggca atcgccctgc
     7561 tgttgcctga cttttaaaca ggcatccagc agggccggac ccgctgcgcg atgaatggcc
     7621 ccatcgacgc cgccgcctcc cattaatgac ggattagccg cattcacaat cacatcaacg
     7681 gccagtttgg taatatcacc ctgcacaaca tgaatacgcg ttttcataac gcctccttaa
     7741 caccttcttg tttcttaagt gtacagcagg ctttttggtt aaatcgcatt tttctactca
     7801 gcaacgtgcg ttgttcagaa acttagttat attcgatgtt cattacagcc agctttttct
     7861 gtttatcaat atactgcact tttactgagg ctacttgcgc gtttttgaca ttgccgctca
     7921 ttaacgcttt gctggaatag gtttttcccg gaacagaacc attctgtggg cagttcattt
     7981 ctacgggtga tgactgggtg ctgacgtcac atgccggttc cacaatttgg ccataaaaat
     8041 ggatcacgcc cccttgcgcg gtgcttcctg cgatggcaat aaaaggaaaa aaaaactgcc
     8101 gagcataaga gagtttaaaa aaggtctgaa catttattct atttcctcaa tgattagtca
     8161 tccttgaggg ttgtgttatc catactttca ggttggctga ttcgcagcag accattctct
     8221 ccagattcat cttatgctcg acatttcaac aaattaagac ttttctgaag agggcggcca
     8281 ttgttgtgat aaatgaagtg cctgtccatc agaaacagta acaactattt tcacccgatc
     8341 ttccggggaa atatttaaac tcaacttcgt caaagcaata ggttgattag caggcaatga
     8401 gagggtcttt tcttgcttcg tctgactttg ccctgaaccg ccttcgcgca gg
//
LOCUS       DAJTMC010000174         8312 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.174, whole genome shotgun sequence.
ACCESSION   DAJTMC010000174 DAJTMC010000000
VERSION     DAJTMC010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8312
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.174"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(95..1765)
                     /gene="hscC"
                     /locus_tag="OO130_003548"
     CDS             complement(95..1765)
                     /gene="hscC"
                     /locus_tag="OO130_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HCR5366813.1"
                     /translation="MDNAELTIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDEQRKHTRLAAELAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEIHASAGDSFLGGEDFTHMLVDEVLKRA
                     EVARTTLNESELAALYACVEAAKCSNQSPLHIRWQYQEETRECEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKKTGAYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKQEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRRASDYLAIEIP"
     gene            complement(1849..2784)
                     /gene="rihA"
                     /locus_tag="OO130_003549"
     CDS             complement(1849..2784)
                     /gene="rihA"
                     /locus_tag="OO130_003549"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HCR5366814.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHIEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTDNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            complement(2902..3627)
                     /gene="gltL"
                     /locus_tag="OO130_003550"
     CDS             complement(2902..3627)
                     /gene="gltL"
                     /locus_tag="OO130_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
                     /protein_id="HCR5366815.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            complement(3627..4301)
                     /gene="gltK"
                     /locus_tag="OO130_003551"
     CDS             complement(3627..4301)
                     /gene="gltK"
                     /locus_tag="OO130_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HCR5366816.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNIFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            complement(4301..5041)
                     /gene="gltJ"
                     /locus_tag="OO130_003552"
     CDS             complement(4301..5041)
                     /gene="gltJ"
                     /locus_tag="OO130_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HCR5366817.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAIIMLVMTLVERKVRLPGNMGGK"
     gene            complement(5211..6119)
                     /gene="gltI"
                     /locus_tag="OO130_003553"
     CDS             complement(5211..6119)
                     /gene="gltI"
                     /locus_tag="OO130_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HCR5366818.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKDKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            6574..>8312
                     /locus_tag="OO130_003554"
     CDS             6574..>8312
                     /locus_tag="OO130_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HCR5366819.1"
                     /translation="MSASSVKPLNVQLPAITLILFALCIGIFCYLAQWMSYEEVDQSA
                     LIHLGANVAPLTLSGEPWRLLSSIFLHSSVSHLLMNMFAFLVVGGVAEQILGKWRLLI
                     TWLFSGVFGGLISACYALRDSDQIVISVGASGAIMGIAGAAIATQLASGAGTYHKNQR
                     RVFSLLGMVALTLLYGARQTGIDNACHIGGLIAGGALGWLSARLSGQNRLVTEGGIIV
                     AGSLLLTGAIWLAQQQMDESVLQVRQSLREAFYPQEIEQERRQKKQQLAEERNALRET
                     LSAPVSREQASGDLLAEIADIHDMAISRDGNMLYAAIENTNSIVVFDLGQKKILHTFT
                     APIAKEKSVKHCGGCKDQGVRSLALSPDEKLIYATSFEANALSVINVATGEIIQSITT
                     GAHPDSLILSRDGTKAWVMNRTSNSVSAIDLVTYQHVADIPLEKYDGAGTSGKPGAWV
                     MALSPDERTLLVPGAGRGNIVRINTITHQKEDFPAGDARGTISAMRFRPENGEVIFAD
                     SQGISRISVGDQQASIMTQWCSRSVYSVEGISPDGQYLALVSYGLQGYVILLNINAGQ
                     IIGVYPASYVNHLR"
BASE COUNT         2073 a         2054 c         2077 g         2108 t
ORIGIN      
        1 atgctctcgg caaaatcgcg atgttgttta gttgacgatg tgttttttgc gccgatgccg
       61 gatgcagcgt ttacgccgca tccggcagcc gtgtttatgg gatctcaatg gctaaataat
      121 cgctcgcccg ccgccgaact tcatccacgc gggccatgtc gtttgactgt agcgccgcat
      181 caaaatcggt gatcacccgg ccaatctctt ctcgctcgtc acccagcgct cgcgcccaca
      241 actcttcctg tttggcttta aaggtgcgat tgatgagcat atcgcgcgga taaattttca
      301 atgcggataa ccgcgtccga ctctcttcaa tctgctgcgc gctcaatgtt accgggctgt
      361 ggttaatcac tctggactta acgctgccgt cttccagaag cacgtcaact tcaagcaacc
      421 cgttgatatc ataactaaag cgaatatcaa tcgactgata agccccggtt ttcttcaacg
      481 gcacatcgaa ggattcaacc agaatgttat ttttaacttt gtggttttct ccctgataga
      541 cgttaaccgt aattgaatcc tgctccgggt gcatggtcga gtaagtttct acacgcgaca
      601 ccggtactgt ggtgtttcgt tcaataatcg gcgagaaaat gccggaaacg ccctggcggt
      661 taacttcaac gcccaacgag taagggcaaa tatcagtgag gattacctct tcaatatctt
      721 cactgcgtaa gcggcaggcg gcctggattg ctgcgcccag cgcgacaatg gtgctcggat
      781 cgtaactttg atacggtaat ttgccaaaca gacgcacggc gattcgctgc accagcggca
      841 tttgtgacgc gccgccaacc agcaccagac tgtcgatttg actcggcttc agacgcgcat
      901 cgcgcaacgc ctgttcaatc ggcactcgca agcgattgag cagcggcaac cacaaatctt
      961 ccagttcgtt ctcgtaaaat tcgcattccc gcgtttcttc ctgatactgc cagcgaatgt
     1021 gcaatggcga ttgattgcta cattttgccg cttccacaca ggcgtacaag gctgccagtt
     1081 cactctcgtt aagcgtggtc ctggcaactt ccgcgcgttt taaaacctca tcgaccagca
     1141 tatgggtaaa atcttcgcca ccgaggaagc tatcgccagc ggaggcgtga atttcaatca
     1201 ccggcgtggc gtactcaagc accgtaacgt caaacgtgcc gccaccgaga tcaaaaacca
     1261 gcgagcgggt attttgttgc gtatgcaggc catatgccat cgcagctgct gtgggttcat
     1321 taattaagcg taccgcattt aacccggcta actccgcagc taaacgggta tgcttgcgtt
     1381 gttcatcgct gaaataagcc ggaacggaga tcaccacatc tttaatcgga cgttgcagaa
     1441 attcttcggc atcttctttt aatgagcgta ataccaaaga ggacagttct ggcgcgttaa
     1501 atgtgtcgct gcctaaccgc cagttggtat tactgcccat tgcgcgttta aataacgctg
     1561 ccgttttatc cggatgcgaa gtacgccgtg atacagccgg ttttccgact aaaatatgat
     1621 tattttcatc catgctaatt atggatggtg ttaaatattc accgaactta tttggaatta
     1681 attgcgcggc accgtctttc cagacggcaa ttaaactatt ggtagtgccg agatcgatac
     1741 caatggtgag ttctgcatta tccattgcat ttatatcttg aaagaaaaag agataaacag
     1801 attaagacca cccaggtaac gcgtggtgat cttaatcaat gacgtgtgtt aagcgtaaaa
     1861 tttcagacga tcagccagta aatcaacaaa gccctgacga tcaacatcga ccattacagt
     1921 ggcattcggt ttattgtcag tcagataata ataatcaaca accgtcatac cctgggtata
     1981 tttcccctgt gtttccacgc caacccagcg ctcaacagtg gtaaacaact cgggtttcaa
     2041 cagccaggcg atggtgcatg ggtcatgcag cggtgcaccg acaaagcccc atttttcgtc
     2101 tttatgatat tcgaggaaga aatccagcag ttcggcaaca atggttgaaa cagggttgcc
     2161 aatcgcgcgg aaacgctcgg tgtcttcaat gtggatttgc gctttatgag taacatccag
     2221 accggccatc accactggga tccctgactg gaagacaatt tctgccgctt ccgggtcaac
     2281 gtaaatatta aattcagccg caggcgtcca gttaccaagc cccattgcgc cgcccataat
     2341 cacgatacgg gcaattttgc tatgcagttc cggatggcta ttgagcagca aggcaacgtt
     2401 agtttgcggt ccggtagaca caatcgtgac aggttccgca ctttcgcgca gcgttttcgc
     2461 catcagctct accgccgtac agttttgcgg tgcgaacgtc ggttccggta atgccgggcc
     2521 atccagaccg ctttcgccgt gcacattgtc cgcgataatc aactcacgca ttaacggttt
     2581 taccgcgccg cctgctaccg gaatatcggt gcgattaagc aaggtcagca tacgcagaac
     2641 attgcgtaag gttttttctg gtgtctggtt tccggcggaa gacgtaattg ctttgacatc
     2701 aagctctggt gaggcgaggg cgagaactat tgcgatagcg tcgtcatgac ctgggtcgca
     2761 atctaacaga attggcagtg ccattgttgc tccttgttgt gtgcttcttt gcgacaaggg
     2821 taacgccagg atgtaacaga tacgaggggc gaaacgataa agcgtgagat ggcgcgcact
     2881 tgggtatgcg cgccagagtg attaatgcag gattttcgcg aggaagtctt tcgcgcggtc
     2941 cgatttcgga tcatcgaaga aagcgtcttt cggcgagtct tcgacaattt taccctcgtc
     3001 cataaagatc acccgattcg ccactttgcg ggcaaagccc atttcgtggg tcaccaccat
     3061 catggtcatt ccttcgttcg ccagttccac catcacgtcc agcacttcgt tgatcatctc
     3121 cggatccagc gccgatgtcg gttcgtcgaa cagcatcgca ataggatcca tacacaacgc
     3181 gcgagcgatt gccacacgct gctgctgacc gccggaaagc tgcgccggaa atttattggc
     3241 atgtgcggaa agtcccacgc gctccagcaa tttcaatgct ttttcacgag cgggcgcttt
     3301 atcgcgttta agcactttca cctgcgccag agtcaggttt tcaataatcg acaaatgggg
     3361 gaacaactca aaatgctgga acaccatccc gacgcgggaa cgcagctttg ccagatcggt
     3421 tttcttgtca ttaaccacga taccatcgac ggtgatttca ccttgctgca ctggttcgag
     3481 gccgttgacg gttttaatca gcgttgattt gccggaacca gacgggccgc aaaccaccac
     3541 cacttcgcct tttttcactt cggttgagca gtcggtcagc acctgaaagt gaccatacca
     3601 ttttgaaaca tttttcaggg taatcattat gctgtccttc ttttcaagta gctgaccaac
     3661 aacgacgcgc taagactaat aacgaaataa acaaatccgg caaacaggat catctcaacc
     3721 tgcgtaccat cacgctcacc aatggttgag gcggtacgga agaaatcggc cagagataac
     3781 acatacacca gtgaggtatc ctggaacagt acgatgccct gagtgagcag aagcggcacc
     3841 atcgcgcgga acgcctgcgg cagaataatc agtttcatcg actgccagtg agtcattccc
     3901 aacgccagcg cggcgctcga ctgaccacga gaaatacttt gaataccagc acggataatc
     3961 tctgaatagt aggccgcttc aaacatcgaa aacgccacca tcgccgaaat taaacggata
     4021 tcattttttg gtgataatcc cagcacgttt tgcagaaaac ctggcacgat caggtaaaac
     4081 cacagcaaaa ccataactaa aggaatcgag cggaatatgt taacgtaggc tttggcaaac
     4141 cacgctacgg gcgcaaagct ggacaagcgc atcaccgcca gcatggtgcc ccacaaaata
     4201 ccaatcacga ctgccgtgac ggtgattttc agcgtgatca ccagcccgtc gagcagatat
     4261 ggcagggaag ggacaatgga actccagtca aactcgtaca ttatttgccc cccatgttgc
     4321 caggcaggcg aactttacgt tcaaccagcg tcattaccag catgataatc gcgttaatca
     4381 acacatacgc cagcgtaata gcggtaaacg actcccaggc gtgggctgag taatcaagca
     4441 atttacccgc ctgcgccgcc atatccacca gaccaatagt cgaggcgatg gcggagtttt
     4501 tcaccaggtt catcatctcg gaagtcatcg gcgggacgat aacgcgataa gcattaggca
     4561 gcagtacgta tcgataagcc tgcggtagcg tcaggcccat cgccagcgcg gcattttttt
     4621 gcccacgcgg caacgactga atggcggcgc gtacctgttc gcaaacacgg gcggcggtaa
     4681 acagccccag gcagagcatg gatgaaagga aaaactgaat attgggatcc agctcggcct
     4741 taaaccacat gccgattttc tccggcaaca gctccgggat caccagatac caggtaaaga
     4801 attgcacaat caacggcacg ttgcggaaca gttcgacata caacgtaccc agaccggaga
     4861 gaaaacggtt tggaacggta cgtaaaatgc cgaaaaatga accgacgagg aaagcgataa
     4921 tccaggcgca gatcgacaaa gcgatcgtca cctgaaaacc actccagatc caaccaagat
     4981 aggtggtgtt gccgaacggg gcttgttgta aaaaaatacc ccagttccag tctatagaca
     5041 taaatctact ccagaaaaaa gagggtagca gcgttaactg ctaccctcga agattgttac
     5101 ccagcgtatt gcggttttca ggcccgatgg ggaacgacca tcaggcgtat agtctgtccg
     5161 tgctacgtaa caatcgagag ggctggaatt tccgcccctg gttcttgtaa ttagttcagt
     5221 gctttgtcat tcggttcttt gaacagtgct ttcatttcgt ctgacagttc gaaattcatg
     5281 ttcaggtttt tcggcggaat tggatttttg aaccatttat caaaccattt ttccgcttca
     5341 ccggaagtct gcacctgagc gatagtgtca tccatcagct ttttgaactg cggatcatct
     5401 ttacgcaaca tgcaaccgta ggcttcctga gactgcggct tgccgacgat atcccagttg
     5461 tctggtttct tcgctttcgc acgttcaccg gccagcaggg catcatccat cataaaggca
     5521 acggcacgac cgctttccag ggtgcggaaa gagtcaccgt gatctttggc gctgatgatg
     5581 cgcatattca ttttttgctc ttcattcagt ttgttgagca aaacttcaga ggtagtgccg
     5641 gaagtgacga ctacggcttt gtctttcagg tcggcaaaat ctttgatatc gccgcccttt
     5701 ttggtcagca ggcgcgtacc gaccacgaaa atagtatcag agaaagccgc ctgtttttgg
     5761 cgttcgacgt tgttggtggt agaaccacat tcaaaatcga aagtgccgtt ttgcagcagt
     5821 ggaatacggt tttgtgaggt aatcggaatc agttttacct gcaagtccgg tttgttgagt
     5881 ttctttttca ctgcttcaac aatggcgttg gagtaatcct gcgagtaacc caccactttt
     5941 tgctgattgt cgtaataaga gaaaggtact gaagattcac ggtgaccgac gacaatcaca
     6001 ccgtttttgg cgattttgtc cagcgtactg cctgctgccg gggcggcgtc atctgcctgt
     6061 gccagtcctg cggaaagcgc cagggcgagg attgctgtgg caggtttacg taattgcata
     6121 tccaactcct ttatcttctg cgttaagaac gcatggatac ccgttgtgag tgtttgtgtt
     6181 gttatcgtct gcaactttat tgtgcagtgt tgtgcctgtt aggtaacatt tagtttggct
     6241 aaatgtaaag atattgctgt tttattgttt gtttttgcga gatgcgccgc accattccga
     6301 agcaaaattc ttaaaccgca cctttttggt gctaccgctg atttgtggtg gtgcaacctg
     6361 gttgcgtcgc ggctgatttc atcgcttaca cagtaaataa agcaacttac atgccaaaaa
     6421 atacttaggg gcaaattaat tacgattgta attttaggct gaaaaagtca tatttcggac
     6481 agaattatct tttcggcatg ggacgtttgc cgggatgata actgcacacg ggaaaaatgc
     6541 tttttacctg tcttttcaag gaagaaacac cttatgtctg catcttcggt taagccgtta
     6601 aatgttcaat tacccgcaat aacccttatc ctttttgcac tctgtattgg gatattttgt
     6661 tatctcgcac aatggatgag ttatgaagag gtcgatcaat ccgcacttat ccatctcggc
     6721 gcaaacgttg cgcctcttac tttgtcgggt gaaccctggc gcttattgag cagtatcttt
     6781 ctgcacagta gtgtttctca tctgctgatg aatatgtttg cattcctggt cgtgggaggc
     6841 gtggcggaac agattctggg gaaatggcga ctcctgatta cctggttatt ctccggcgtc
     6901 tttggtgggc tcatcagcgc ctgttatgcg ttacgcgata gtgatcagat agtcatcagc
     6961 gttggggcat ccggggcaat tatgggaata gctggcgctg cgatagcaac acagcttgct
     7021 tcaggtgcgg gcacatacca taaaaaccag cggcgagtat tttctctgtt gggtatggtg
     7081 gcgctgacac tgttgtatgg tgcccggcaa acaggaatag ataatgcttg tcacattggc
     7141 ggcctgattg cgggtggcgc gttgggttgg ctgagcgcgc gtttatctgg gcaaaaccga
     7201 ctcgttacgg aaggcgggat tattgttgcg ggcagtcttc ttctgaccgg ggctatctgg
     7261 cttgcgcagc agcagatgga tgagtcagtt ttacaagtca ggcaaagcct gcgtgaagcg
     7321 ttttatccac aggagattga acaagagcga cggcaaaaaa agcagcagtt agcggaggaa
     7381 cgcaacgccc tcagggaaac attatccgct ccggtaagtc gtgaacaggc cagtggtgat
     7441 ttgctcgctg agattgccga tatccatgat atggcgatca gtcgggatgg taatatgttg
     7501 tatgccgcaa ttgaaaacac gaacagcatt gttgttttcg acctcggaca aaagaaaatc
     7561 ctgcatacct ttacagcgcc catagcgaaa gaaaagtcag tcaaacattg tggtggctgt
     7621 aaagatcagg gcgtcagatc gctggcatta agcccggatg aaaagttgat ttatgcgact
     7681 tcatttgaag cgaatgcgtt atcggtcatt aacgtggcga caggggagat tattcagtcg
     7741 attaccaccg gtgcacatcc tgacagcctt atcctctcgc gtgatggcac aaaagcctgg
     7801 gtgatgaatc gcaccagtaa tagtgtgtca gcgattgatc tggtgactta tcagcatgtg
     7861 gcggatatcc cgctggagaa atacgacggg gcggggacga gcggtaaacc aggcgcctgg
     7921 gttatggcac tttccccgga tgaaagaaca ttactggttc cgggagcagg cagaggtaac
     7981 atcgtgcgga tcaataccat cacgcatcag aaagaagact ttcccgcagg tgatgcgcgt
     8041 ggaacgatat cggcgatgcg ttttcgacct gaaaacggcg aggttatttt tgcagatagt
     8101 caggggattt cacgtataag cgtaggggat caacaagcca gcattatgac gcaatggtgt
     8161 agcaggagcg tttattccgt tgagggtatt agcccggacg gtcaatattt agcgttggtg
     8221 tcatatggcc tgcaaggtta tgtcatcctg ctcaatatta atgccgggca gattattggc
     8281 gtttatcctg ccagctacgt taatcacctt cg
//
LOCUS       DAJTMC010000175         8193 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.175, whole genome shotgun sequence.
ACCESSION   DAJTMC010000175 DAJTMC010000000
VERSION     DAJTMC010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8193
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.175"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(<1..815)
                     /locus_tag="OO130_003555"
     CDS             complement(<1..815)
                     /locus_tag="OO130_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR5366820.1"
                     /translation="MKPAHNPSFFRSFCGLGCISRLSVEEQNITDYHRIWDNWAKEGA
                     ATEDRTQAVRLLKICLAFQEPALNLSLLRLRSLPYLPPHIQELNISSNELRSLPELPP
                     SLTVLKASDNRLSRLPALPPHLVALDVSLNRVLTCLPSLPSSLQSLSALLNSLETLPD
                     LPPALQKLSVGNNQLTALPELPCELQELSAFDNRLQELPPLPQNLRLLNVGENQLHRL
                     PELPQRLQSLYIPNNQLNTLPDSIMNLHIYADVNIYNNPLSTRTLQALQRLTSS"
     gene            907..993
                     /locus_tag="OO130_003556"
                     /pseudo
     CDS             907..993
                     /locus_tag="OO130_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1294..1383
                     /locus_tag="OO130_003557"
                     /pseudo
     CDS             1294..1383
                     /locus_tag="OO130_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311139.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 family protein"
     gene            1407..5921
                     /locus_tag="OO130_003558"
                     /pseudo
     CDS             1407..5921
                     /locus_tag="OO130_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
     gene            5922..7571
                     /locus_tag="OO130_003559"
     CDS             5922..7571
                     /locus_tag="OO130_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HCR5366821.1"
                     /translation="MNWRRIVWLLALVTQPTLAEETPLQLALRGAQHDQLYQLSSSGV
                     TTVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPGVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIHTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QDLIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            7576..>8193
                     /locus_tag="OO130_003560"
     CDS             7576..>8193
                     /locus_tag="OO130_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5366822.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKIQQGRLVVNGASMPQRVESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQV"
BASE COUNT         2001 a         1968 c         2301 g         1923 t
ORIGIN      
        1 gaagaggtta atctttgcag ggcttgcaga gtgcgagtcg acaatggatt gttataaata
       61 ttaacatctg cataaatgtg cagattcatg atactgtctg gcaatgtgtt cagctgattg
      121 ttagggatat atagtgattg cagacgttgt ggaagttcgg gcagtctgtg tagttggttt
      181 tccccaacgt ttaaaagcct cagattttga ggaaggggcg gtagctcttg taatctgtta
      241 tcaaaagcac ttagttcctg tagttcacat ggtaattctg gtaaggcagt aagctggttg
      301 ttgccaacag aaagtttttg tagagccggg ggaagatcag gtagcgtctc caggctattg
      361 agaagggctg agagtgactg caaggaagat ggaagagaag gtaaacatgt taaaactctg
      421 ttaagtgaaa catcaagagc gaccaggtga ggcggaagag ccgggagcct gctcagtctg
      481 ttatcgctgg ctttaagtac agttaaggac ggagggagtt ctggcagaga gcgtagctca
      541 ttgctagaga tgttaagttc ttgtatgtgc gggggcaggt atgggagaga gcgtaatctg
      601 agtaaactta aattgagggc tggctcttga aaagccagac atattttcag taatcgaact
      661 gcctgtgttc ggtcttctgt tgcagcacct tccttggccc agttatccca gatgcggtga
      721 taatccgtga tattttgctc ttctacggat aaacgggata tacatcctaa accacaaaag
      781 gagcggaaaa aagaaggatt gtgggcaggt ttcatttatt attgatcctc attgtttata
      841 tattaatatg ttcgtgcaaa tggagtgagg attctttaaa aaaacgctcc tgtaaatagt
      901 taagaacagg aagaactgtt ggggatgtgg ctggccgaaa atgaaggggt gaagttctgg
      961 ctcaatgtgc tgactgaact gaaaaaacgc ggttggtcgc cctacctttt catcaagcca
     1021 gtagtctgcc caccactgca tcatttctct gcgcttatcg agatactgag catggttgta
     1081 aattcaacta acccagtgcg ttgacaaaaa attagcgcaa gaagacaaaa aatcaccttg
     1141 cgctaatgct ctgtatcagg tcactaatac cagctaagta gttgattcat agtgactgca
     1201 tatgttgtgc tttgtcgcat tttgtagtct attatttaat ctgtaacaac gataatacaa
     1261 tgaattttaa ttggtttttg tgattttgtc cttcttctga catggaagga cacccgatgg
     1321 atacccaacg attccaatcc caatttcatt ggcatttatc gtttaaattt tctggcgcga
     1381 tagcagcgtg cttgtctctg tctcttgtgg gaacagggct tgctaatgct gatgattcgc
     1441 ttccttccag taactatgcg ccgcccgccg ggggaacatt ctttttgctt gctgacagca
     1501 gttttggcag cagtgaagag gcgaaagtgc gactggaagc gccggggcgt gattatcggc
     1561 gctatcagat ggaagagtac ggcggcgtgg acgttcgcct gtatcgtatt cctgacccga
     1621 tggcattttt gcgccagcag aaaaacctgc atcgcattgt ggtgcaaccg caatatctgg
     1681 gcgacgggct gaacaatacg ctgacctggc tgtgggataa ctggtacggc aaatctcgcc
     1741 gcgtaatgca gcgtactttc tcttctcagt cacggcagaa tgtgactcag gcattacccg
     1801 aattacatct cggcaatgcc attattaaac cttcccgtta tgtacagaac aaccagtttt
     1861 ccccgctgaa aaaatatccc ttggtggaac agttccgtta tccactatgg caggctaaac
     1921 cggtcgagcc gcagcaaggg gtaaaactgg aaggcgcatc cagcaatttc atctcgccgc
     1981 agccgggtaa catttatatt cctctcggcc aacaagagcc gggactgtac ctcgtcgagg
     2041 cgatggttgg tgggtatcgg gcgacgacgg tggtgtttgt ttccgatacc gtggctctta
     2101 gcaaagtgtc aggcaacgag cttctggtgt ggaccgcggg taaaaaacag ggtgaagcga
     2161 agcccggctc agagatcttg tggactgacg gtcttggcgt gatgacccgc ggtgtgaccg
     2221 atgacagcgg taccttgcag ttacaacata tatcgccaga acgttcatac attctgggta
     2281 aggatgctga aggcggcgtt tttgtctccg agaacttctt ctacgaaagc gaaatctaca
     2341 acacccgctt gtatattttt accgatcgcc cgctatatcg cgcaggcgat cgtgtcgatg
     2401 ttaaagtgat gggccgcgag ttccacgatc cgttgcattc atcccccatc gtcagcgccc
     2461 cggcgaagct ttcggtgctg gacgctaacg gcagtctgtt gcaaaccgtc gatgtcacgc
     2521 tggatgcgcg caatggcggg cagggaagtt tccgcctgcc agaaaatgcc gttgccggag
     2581 gttatgagtt acgtcttgct taccgcaatc aggtctatag cagcagtttt cgcgtggcaa
     2641 actacatcaa gccacatttc gagattggtt tagctctcga caaaaaagag ttcaaaactg
     2701 gcgaagcggt cagcggcaaa ctgcaactgc tctacccgga tggcgagccg gtaaaaaatg
     2761 cccgcgtgca gttaagtttg cgcgctcagc aattatcaat ggtcggtaac gatttgcgtt
     2821 atgccggacg tttccccgtg tcgctggaag gcagcgaaac ggtgtccgac gccagcggtc
     2881 atgtggcgtt aaatctcccc gccgccgata aaccgagccg ttatttgtta accgtctccg
     2941 ccagtgacgg cgcggcgtat cgtgtcacca ccaccaaaga gatcctcatt gaacgcggtc
     3001 tggcgcatta ctcattaagt actgccgcac aatacagtaa tagcggcgag tcggttgtgt
     3061 tccgttatgc cgcgctggaa tcttcaaaac aggttcctgt tacgtatgaa tggttgcgtc
     3121 tcgaagaccg cacgagccat agcggagagc taccgtcagg cggcaaatcc tttaccgtca
     3181 atttcgctaa acctggcaac tacaatctga cattacgcga taaagacggc ttaattctcg
     3241 ctgggttaag ttatgccgtc agcggtaagg gcagcacggc gcatacgggt acggtagata
     3301 tcgtggcgga taaaacgctg taccagccag gcgaaaccgc gaagatgctg attacctttc
     3361 cggagccaat tgatgaagca ttattgacgc tggaacgcga tcgcgtggaa cagcagtcgc
     3421 tgctttcgca tccggcaaac tggctaacgc tacaacgttt aaacgatacc cagtatgaag
     3481 cccgggttcc agtgagcaat tcctttgcgc ctaacatcac tttttcggtg ctgtataccc
     3541 gtaatggtca gtacagtttt cagaacgccg ggatcaaagt tgccgttcct cagcttgata
     3601 tccgggtgaa aacggacaaa acccattacc agccaggtga actggtcaat gtcgaattaa
     3661 cctcgtcgct gaaaggtaaa cctgtttctg cgcagctaac ggtaggcgtg gtcgatgaaa
     3721 tgatctacgc gctgcaacca gaaatcgcgc cgaatatcgg caaatttttc tatccgctgg
     3781 ggcgtaacaa tgtgcgtacc agctccagtc tgtcgtttat cagctacgac caggcgctct
     3841 ccagcgagcc ggttgcgcct ggcgcaacta accgcagcga gcggcgagta aaaatgcttg
     3901 aacgtccacg gcgtgaagag gtggataccg cggcatggat gccgtcactc acaaccgata
     3961 aacaaggcaa agcgtatttc acgttcctga tgcctgattc gttaacccgc tggcgtatca
     4021 ccgcgcgtgg gatgaacggc gacgggctgg tcgggcaggg gcgtgcttat ctgcgttcgg
     4081 aaaaaaatct ctacatgaag tggagtatgc caacggtgta tcgcgtgggc gacaaaccgg
     4141 cggcaggact gtttatcttc agtcagcagg ataacgaacc ggtggcgctg gtgactaaat
     4201 ttgcaggcgc tgagatgcgc cagacgctga cgctgcacaa aggggcgaat tatatttcgc
     4261 tggcgcagaa tattcagcaa tctggcttgt taagtgcaga actgcaacaa aatgggcaag
     4321 tgcaggacag cattagcaca aaactgtctt ttgtggacaa cagcaggccc gttgaacagc
     4381 agaaaaatgt catgctcggc ggtggcgaga acgcgctgat gttgcccgag caggcgagta
     4441 atatccggct acaaagtagt gaaacgccgc aggagatttt ccgcaacaat cttgatgcgt
     4501 tagtcgatga accgtggggt ggggtgatca acaccggtag ccgtctgatc ccgctcagtc
     4561 tcgcctggcg ttcgcttgcc gatcatcaaa gtgccgccgc taacgacatt cgtcagatga
     4621 ttcaggataa ccgtctgcgg ctgatgcaac tggcggggcc cggagcgcgc tttacctggt
     4681 ggggtgaaga tggcaatggt gacgccttcc ttacggcatg ggcatggtac gccgactggc
     4741 aggccagcta ggcgctcggc gtaacgcaac aaccggaata ctggcagcat atgctcgaca
     4801 gttatgccga gcaggcggat aacatgccgt tattgcatcg ggcgctggtg ctggcgtggg
     4861 cacaggagat gaatctgccg tgcaaaacgt tgttgaaagg gttggatgaa gctatcgccc
     4921 ggcgcggaac taaagatgaa gatttctctg aggaagacat ccgcgatatc aatgacagcc
     4981 tgatcctcga tacaccggaa tctccactgg cagatgcggt ggcaaacgtc ttaaccatga
     5041 cgttgctgaa aaaagcgcag ttgaagtcca cggtgatgcc acaggttcag caatatgcgt
     5101 gggataaagc ggcaaacagc aatcagccgc tggcgcacac ggttgtgctg ctcaatagcg
     5161 gtggtgacgc tacccagacg gccgctattt taagtggttt gaccgctgag caatccacta
     5221 ttgagcgcgc gctggccatg aactggctgg cgaaatatat ggcgacaatg cctccagttg
     5281 ttttgcctgc gcctgcgggc gcatgggcta aacataagtt aactggaggg ggcgaatact
     5341 ggcgttgggt tggtcagggc gtgccggaca ttctctcttt tggtgacgaa ttatcgccgc
     5401 aaaatgtgca ggtccgctgg cgtgagccgg caaaaatggc tcaacaaagt aacattccgg
     5461 tgaccgttga acgccagttg tatcggctta tccctggtga agaagagatg agctttattc
     5521 tgcaaccggt gaccagcaat gagattgaca gcgatgcgct gtatctcgat gacatcacgc
     5581 ttaccagcga gcaggatgca gttctgcgct acggtcaggt ggaagtaccg ctgccaccgg
     5641 gagccgacgt tgagcgcaca acatggggca tttcggtcaa taaacccaac gtcgcgaaac
     5701 agcaggggca attgctggaa aaagcgcgaa atgaaatggg cgaactggcc tatatggtgc
     5761 cggtgaaaga actgacggga acggtcactt tccgccattt gctgcgcttc tcgcaaaaag
     5821 ggcaattcgt tctgcctcct gctcgttatg tgcgttccta tgcacctgca cagcaaagtg
     5881 ttgcggcagg gagtgaatgg accgggatgc aggtgaaata agtgaactgg cgcagaatcg
     5941 tctggctatt ggcgttggtt actcagccaa cgcttgcgga ggagacgccg ttacaactgg
     6001 cactgcgtgg tgcgcagcac gatcaactgt atcagctgtc gtcatcaggc gtgaccacag
     6061 tctcagcatt acctgactcg ctcaccacgc cgttagggag tttgtggaaa ctgtatgttt
     6121 acgcctggct ggaggatacc catcaaccgg agcagcccta ccagtgtcgc ggaaactccc
     6181 cggaagaggt ttattgttgt caggctgggg agagcataac gcgcgatacc gcgctggtac
     6241 gttcctgtgg gctctatttt gcgccgcagc ggttgcatat tggcgcagat gtgtgggggc
     6301 aatactggca acagcgtcag gcgcctgcgt ggttagcgtc tcttactacg ctgaagccag
     6361 aaacctcggt aacggttaaa tcgctgcttg attcattagc gacgttacct gcgcaaaaca
     6421 aagctcagga agtcttactg gatgtggtgc tggacgaggc gaaaatcggt gtcgcatcca
     6481 tgctcggtag tcgggtacgg gtgaaaacct ggagctggtt tgccgatgat aaacaggaaa
     6541 tccgtcaggg tggttttgcc ggctggttaa ccgatggtac accgctgtgg gttactggca
     6601 gtggcaccag caaaacggta ctaacccgtt atgcgacagt gcttaatcgg gttttgccag
     6661 tgccgacgca ggttgccagt gggcaatgtg tagaggtgga gctgtttgcc cgctatccgc
     6721 tgaagaaaat cacagcggaa aagagtacga cggcagtaaa acccggcgta cttaatgggc
     6781 gctatcgtgt gacgtttact aatggtaacc acattacgtt tgtcagccac ggtgagacga
     6841 ccttactgag cgaaaagggc aaactgaaat tgcagtcgca tctcgatcgt gaagagtacg
     6901 ttgccagagt tctggatcgc gaggcgaaaa gtacgccccc tgaggcagcg aaagccatga
     6961 ccgtggcgat tcacactttt ttacagcaaa acgccaatcg ggaaggcgat tgcctgacca
     7021 tccccgacag cagcgccacg cagcgtgttt ctgcttcgcc agcgaccacg ggggcgcgaa
     7081 cgatgaccgc ctggacgcag gatctcatct atgcaggcga tccggttcat tatcatggca
     7141 gtcgagctac cgaagggaca ctttcctggc ggcaagccac ggcccaggcg gggcagggag
     7201 agcgttacga ccagatactc gcgtttgctt atcccgacaa cagccttagc cgctggggag
     7261 cgccacgctc aacctgccag ttattaccca aagcgaaagc ctggctggcg aaaaaaatgc
     7321 cgcagtggcg gcgtatatta caaggcgaga cggggtacaa cgaaccagac gtgtttgcgg
     7381 tctgtcgttt agtctcaggt ttcccctata ccgatcggca gcagaaacgg ctgtttattc
     7441 gtaacttctt tacgcttcag gatcggctgg atttaaccca tgagtatctg cacctggcct
     7501 ttgatggtta tcccaccggg cttgatgaga actatatcga aacgctgacc cgacaattat
     7561 taatggacta atgctatgcg aaagattttt cttccgttgt tactggtggc actttcgcct
     7621 gtcgctcaca gtgaaggtgt gcaggaagtc gagattgacg caccgctctc tggctggcat
     7681 ccggcagagg gcgaagatgc cagtttttca caatcgatca attacccggc gtcgtccgtc
     7741 aacatggcag atgatcaaaa tatctcagcg cagatccgcg gcaaaataaa gaattatgct
     7801 gcggcgggta aaattcagca gggccggctg gtggtcaacg gtgccagtat gccgcagaga
     7861 gttgaatccg atggttcatt tgcacgccct tatattttca ctgaaggcag caacagcgtg
     7921 caggtcatca gcccggatgg gcaaagccga caaaaaatgc agttttactc aacgccgggt
     7981 acaggaacga ttcgtgcacg tttacggctg gttctctcgt gggatacgga caataccgat
     8041 ctcgatctcc atgttgtcac gcccgatggc gaacacgcct ggtacggtaa caccgtgctg
     8101 aaaaacagtg gtgcactgga tatggatgtc acgacggggt acggacccga gattttcgcc
     8161 atgccagcgc cagttcacgg acgctatcag gtg
//
LOCUS       DAJTMC010000176         8146 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.176, whole genome shotgun sequence.
ACCESSION   DAJTMC010000176 DAJTMC010000000
VERSION     DAJTMC010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8146
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.176"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            <1..260
                     /locus_tag="OO130_003561"
     CDS             <1..260
                     /locus_tag="OO130_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HCR5366823.1"
                     /translation="VVLGMIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRF
                     YRLNGDTLRRIQIHLLDKYRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(399..1028)
                     /locus_tag="OO130_003562"
     CDS             complement(399..1028)
                     /locus_tag="OO130_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HCR5366824.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATARRYPLTPLLYTLIFLHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRRFGLITG"
     gene            complement(1151..2797)
                     /gene="fumB"
                     /locus_tag="OO130_003563"
     CDS             complement(1151..2797)
                     /gene="fumB"
                     /locus_tag="OO130_003563"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCR5366825.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGIAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRI
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDTCHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            complement(2875..4215)
                     /gene="dcuB"
                     /locus_tag="OO130_003564"
     CDS             complement(2875..4215)
                     /gene="dcuB"
                     /locus_tag="OO130_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HCR5366826.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            complement(4786..5505)
                     /gene="dcuR"
                     /locus_tag="OO130_003565"
     CDS             complement(4786..5505)
                     /gene="dcuR"
                     /locus_tag="OO130_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HCR5366827.1"
                     /translation="MSNVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(5502..7133)
                     /gene="dcuS"
                     /locus_tag="OO130_003566"
     CDS             complement(5502..7133)
                     /gene="dcuS"
                     /locus_tag="OO130_003566"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HCR5366828.1"
                     /translation="MRHSLPYRMLLKRPMKLSTTVILMVSAVQFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGKENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVVSGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYSLKTANNYKEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESLLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            7314..7544
                     /locus_tag="OO130_003567"
     CDS             7314..7544
                     /locus_tag="OO130_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HCR5366829.1"
                     /translation="MDQALLDEGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVVTVVKVIDTCPSGALKYRHK"
     gene            7556..7828
                     /locus_tag="OO130_003568"
     CDS             7556..7828
                     /locus_tag="OO130_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR5366830.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            8055..>8146
                     /gene="ghoS"
                     /locus_tag="OO130_003569"
     CDS             8055..>8146
                     /gene="ghoS"
                     /locus_tag="OO130_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HCR5366831.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRLRSL"
BASE COUNT         2025 a         1968 c         2082 g         2071 t
ORIGIN      
        1 cggttgtgtt agggatgatt ggctatgtac cgaatgttga acagtctacg caagccctat
       61 taggtatgca gtttattatg attgctctac caactctgtt tttcatggta acgctgattc
      121 tctacttccg tttctatcgc ctcaatggtg acacgctgcg caggatccag atccatctgc
      181 tggataaata tcgcaaagta ccgcccgagc ctgttcatgc tgatattccg gtcggtgcag
      241 tgagtgatgt gaaagcctga cgtggacaat agctaacgaa aatggcctga tgcgatatgt
      301 ttatcaggcc acgtcttaat gtaatgcttt ggatttgcaa attttgtaga ccggataagg
      361 aattcacgct gcatccggca tcaacaaagc gcacgttgtt atccggttat caagccaaag
      421 cgccgtagct ggcggcaatg aaagcgagcg aggaatatca ccgtcgttaa tgcgccaagc
      481 agcgcgcaga acatatcgga ttgcgtgtcc cactggtcgc cctgggtgcc gagaaaatca
      541 tccgctccct gacccattgc cagcgccgcc caccactcga ttaattcata cattgcgctt
      601 atcgccagcg ccacgcagca gactaggaac gccaccattt tacgtccgcg cacgtacatc
      661 ccgcgaacga gaatttctcg tgccaccagt gcaggcacca gcccctggaa aaagtgcccc
      721 agcttgtcat acggattacg gctcaacccc agccattcct gcacctcaaa accaacgggg
      781 actttcgcgt aggtgtattg tccgccgacc atcaggatga tggcgtgaag gaaaatgagc
      841 gtatagagca gcggcgttaa cggataacgt ctggcggtgg caagcagtag cggcacgaca
      901 ataatcaccg gtgtcacttc catcagccag gtgagtttgt catgggccga aatgccggta
      961 taaatcagga ttaatgttag cgccagtgcg ccggtgttaa gaattaacgg cttgagtgtg
     1021 cgggtcatgg tgagttcgca aatcagggaa aatactgact attcaccagc aagcgtgaaa
     1081 ttacaatcgc aaaagaggtg gcccaggggg atcacctggc agcatgctgc caggcgctgg
     1141 gccgaagagg ttacttagtg cagttcgcgc actgtttgtt gacgatttgc tggaagaagt
     1201 cgttaccttt gtcatcgacc aagataaacg ccgggaaatc ttctacttcg attttccaga
     1261 tagcttccat acccagctcc ggataagcga cgcattccag atgcttgatg ctctgctgcg
     1321 ccagtaccgc cgccggaccg ccgatgctac cgaggtagaa gccgccgtgt ttatgacacg
     1381 tgtcggtaac ctgctgactg cggttacctt tcgccagcat gatcatgctg ccgccgtggg
     1441 attgcagcag atccacgtag gagtccatac ggcctgcggt ggttgggcca agtgaacctg
     1501 atggataacc ggcaggggtt ttcgccggac ccgcgtagta gatcgggtga tctttgatgt
     1561 actgcggaag ttctttaccg gcgtcaatca gctctttcag cttggcgtgt gcaatatctc
     1621 ggcccacgat aatggtgccg gtgagcgaca aacgagtgga taccgggtat tgcgaaagct
     1681 gggcgaggat ctctttcatc gggcggttaa ggtcaacttt caccgcttcg ccttcaccgg
     1741 cctggcgcag ttcttgtgga atgtactggc ctgggttgtg ttccagtttt tcgatccaga
     1801 taccttcgcg gttgattttc gctttaatgt tacggtcagc ggagcaggag acgcccatgc
     1861 cgaccgggca ggatgcgccg tgacgtggca gacggataac gcgaatgtcg tgcgcgaagt
     1921 atttaccgcc aaactgcgcg ccaagaccga gtttctgggc ctcttccagc agttcctgtt
     1981 ccagctggac atcgcggaac gcctgaccat gttcgttccc ttccgtcggc agttcatcgt
     2041 aatagtgagc gcttgctaac ttgacggttt tcaggttggt ttccgcagac gtaccgccaa
     2101 tcacaaacgc gatatggtac ggcgggcagg ctgcagtacc gaggatacgc attttctcga
     2161 cgaggaagtt tttcagtttg ccgggagtca gcagggcttt ggtttcctgg tagagatacg
     2221 ttttgttggc agagccgccg cctttcgcaa cgcaaaggaa tttgtactca tcgccatcta
     2281 ccgcgtacag gtcgatttgc gcaggcaggt tagtgccggt gttgacctct ttgtacatgt
     2341 ccagcgccgc attctgtgaa tagcgcaggt tatcttcgat ataggtgtta tagacgcctt
     2401 tcgacagcgc ttcttcatca ccgccgccgg tccacacgcg ctggcctttt ttaccgacga
     2461 tgatcgcgat gccggtatcc tggcaggtcg gcagcacgcc tttggcggcg atttcggaat
     2521 ttcttaagaa ttgcagcgcc acgtacttgt cgttttcgct ggcttctgga tcgtgaagaa
     2581 tagccgcaac ctgtttctgg tgtgccgggc ggagcataaa agaagcgtcg tgaaaggctt
     2641 gctgcgccag cagggtcagg gcttctggtt ccactttcag gatggtttcg ccgtcgaagt
     2701 cggcaacgct aacgtaatcg gaagtgagta gatagtattc ggtattgtct ttccccatcg
     2761 ggaaaggtgc ctggtagata aagggtttgt ttgacatagc ttccagcctg taactctgta
     2821 tttgttattc gaaaatggcg tgccgcgtag tcacgggcgg cacgcaaagt gcatttataa
     2881 gaacccgtac atcgcggcga agatccagcc gaagacgcac gatacgctca caccaatcaa
     2941 ccccggcaga ataaagctgt ggttgatgac gaagcgaccg atgtgggtgg tgccggaacg
     3001 gtcaaactga atcgctgcca gatcgctcgg ataagtcggc aggatgtaat aaccgtagca
     3061 agccggtgct gaagccacga tgtatgccgg atcaacgccg atcgccaacg cgaccggaac
     3121 aatcgccgcc agcgccgcag cctgagagtt tacaaacttg gaaaccagca gcagaacaat
     3181 ggcataggcc cacggatact ctttcaccat ttcacccagt acgccctgaa tttcagacat
     3241 atgcgcaccg aacatggttt ctgccatcca tgcgataccg tacaccgcca cgatggcgat
     3301 cataccggaa cggaagactt cgttttttga gatagacgcg ggattggttt tggtcaggat
     3361 aataatcagc gccccggtca gcagcataaa catctgaata accagtacca tcgacagcgg
     3421 tttgccgccg aaggatggac gcaggtccga atcagcacca agaagggcga ctacagcgat
     3481 tgccccgagg aaaatccaca ttgccagcca gttgcttttc ggcagttttt tatccagcag
     3541 cgtcgcggta tcaccgtaaa catactcacg gttttccggt acggagatga atttctggaa
     3601 ctcttcgtct ttatccagat ctttaccacg gaaccagctg aagataccga tcgccaggat
     3661 accgattaac gtcgatggaa tggtgattgc cagcaggtcg aggaactcaa gatggcgacc
     3721 atcaaaggtg acattaccca gcatcgcaac cagagacacg accgcaaccg acaccggact
     3781 ggcgataatc cccatctgtg caccgataga acttgccgcc atcggacgtt ccggacggat
     3841 gttgttctta atggcgacgt cgtagatgat cggcagaatg gtgtaaacca catgacccgt
     3901 accgcaaaga atggtcagcg tacaggtcac aaacggcgcg acaattgaga catatttcgg
     3961 gttgcggcgc agcaacttct cggcaatttg cagcatgaca tcaagaccgc ccgaagcttg
     4021 caaggtcgcc gatgccgcca ccaccgcaat gataaccagc atgacatcaa ccggtggttt
     4081 acctggctga aggtggaaga caaagaccag aatgaccaga ccgataccgc ctaataaacc
     4141 cagcgcgata ccaccctttc tggcaccata aaacagacat atcagtatta tgataagttg
     4201 gatagtaaat aacatgtgtg aaccctcgcg ataatcctat ttaaattttt gctgaataga
     4261 tcacagtcac gttctgtttt gtatgaactg ttttcagagc ctgactaaat atccgtttgg
     4321 tcgttaccgg ctttagcaaa tacctcacag taaatattgg ctgattaaag cagcggcaaa
     4381 tttatgcact gtgataagcg gctttttcag agagaaaaac tctctggcga tgggtttata
     4441 gtttgtactg cgcagatgtt aatgttttgt ttgctgttat cttattgata tttatcggtc
     4501 tattttttgt tctcagggca gatatcatta ggtttaaaga ttaatcttat ctacccattt
     4561 gtgggtgaaa atacacataa tgcgggtgac ataatagtta attaactttt gttagcgttt
     4621 tgaaattaaa aacaccgttc acctgaagag atattaattt ttagcgatga tggagggata
     4681 attatatttg atctggcaca agttttactg atgaaggatg taacttgtgc caggggtatt
     4741 ttgcattacg gtaataatta ttacttacag ataaccagcg atccgttatt ggcaatattg
     4801 tttcagtagt gagtagtgtt ctgcctgaat acggtaacgg taaaccggac gccccgtgac
     4861 gccataatgg atactggtga acaagatgtg gcagttgacc agccagatga ggtatttacg
     4921 gcaggaaaca cgcgagatgt taacctcgtt agccagctcg tcggttgaaa actcatagtc
     4981 ctgatgcgcg tcaatccact ggcacagcgt gcgtaacgtc tgcggcgtta agccttttgg
     5041 caagcgacga ggatcctgtt cgttggagct gctgccgtgg attagctgat caagctcggc
     5101 ctggtcataa tactgatgtt tttccagcgc cattttcttt tgccgccagc cggtgagcgc
     5161 ctcttcaaag cgggaagcct ggaagggttt gatcaggtaa tccacgacac cgtaatgcag
     5221 cgaatcttta atggttgccg catcggctgc ggaggagatg acaatcacat cacttttgca
     5281 acgcgcgtta tgcaggacag gcagtaaatc gagcccgttc tctttttgca tatagatatc
     5341 gagcaatatc aggtcgatag gcgtatcgct attgaagata atctctttgg ctttctccag
     5401 cgtcgaggct gttccacagc attgaaagcc tgggatttgt gctacgtatc ggcgattcag
     5461 ctccgcgacc attgcgtcgt catcgataat taatacattg ctcatctgtt tgacctctcc
     5521 ccgtcccagg gtatctggac aaaaaattgt gtgaaaatcc cgggttccga ttccacggcg
     5581 atgctgccgc cgagattttc tacctgttgt ttgacaagtg ctaaaccgac gcctcgctcg
     5641 cttccttttg tcgagacacc tttgtcaaaa atgtgatcga ttttgtcggg tgcgatcccc
     5701 ggtccatcat cgttaacttc gcagtgcagc cagccgtgac ggtagtgcaa tgttacgcta
     5761 atttcgccac cgggttccgg ccctaatgct tccagcgcgt tttctatcag atttcccaat
     5821 gtggtaatca gcgtcgcgac ctggtcctca ctgccgctgt ctggcagcag gctttcactg
     5881 tttaaaatca gcgtatggcc taaatcggtc gcgcggttaa tcttgctgat taaaaaacca
     5941 gcgataaccg gagatttgat cttacccagc agagagccaa tctcttcctt atagttattg
     6001 gctgttttga gactgtaatc ttccaactgc ttataactct tcagatgcaa taatccgaga
     6061 atcacatgca atttattcat aaattcgtgg gatcgttcac gaagtgcatc agcatagttg
     6121 accagaccgt cgagtcgctg catcagttta cgaacttcag ttttgtccct gaaggttgaa
     6181 atggcaccga tgataacgcc attactgcgc accggaacgg tgttgatcag taatagccgg
     6241 tctttaatcg taatctcttc gtcgcggcgc ggcgtaccgt cgcgtaacac ttccgagaca
     6301 tctaccacct gtgaccatga gtggcttagc gtcgacagtt tctcatcgtc ctgcgactta
     6361 cggtaattca gcaattcttg tgcggcatcg ttgatcagcg tgacctcgcc gcgatcgtcc
     6421 acggcaacga cgccttcttt gatagactgc aacatggcct ggcgctgctc aaacagcgtg
     6481 gagatttcgt agggttccag gccgaaaagg atttttttca gtaccttaac cagaatgcag
     6541 gtgccaatca gtccgaccag catgccaaat aataccgacc agataatgct ccagcgactg
     6601 tcattgattt gttgggtcac gcggcttaac tcaaggccgc tcaccaccac gccaatttgt
     6661 ttatgatttt catcgtagat gggggtaaat acgcgtaaag cctgcgccag aaaaccgcga
     6721 ttgatagcga cattttcttt gccattcagc gctttaagga tgtcatcacc tttaaatggc
     6781 tgaccaatac gctgggcttc aggatgcgag tagcgaagac tttgcatatc cgtaacgaca
     6841 ataaacagca gatcgttgcg tttgcgcacg gcttccgcga tggcctggat tccgctctcc
     6901 tgcggttttt tctgcaagcc ctgacggatt tccggcgagt cggcgagggt acgcgccact
     6961 gccagtgcct tgttggccag cccgtctcgt gtcatatcac tgatttgcga gaagtaaatc
     7021 agatgcacca ccaatagcac cgagaactgt accgcgctga ccattaagat cactgtggta
     7081 ctcaatttca tcggacgttt gagtaacatg cggtagggca atgaatgtct catcagcttc
     7141 cttgtgtgac aaatttctta agcattatct ctgatgaggc gggtaattca aagggagtaa
     7201 gaatgattgg ctatataggg gaagagactc tggcaacgga aactgccagt gctgtatgaa
     7261 gattccaggg ctatgcttat agcgataatc atactgatga gagagggaaa gtcatggatc
     7321 aggcgctact ggacgagggt tatcgctgtt ataccggcga aaagatcgat gtctatttca
     7381 acactgcgat atgtcagcat tctggcaatt gcgtacgtgg caacggcaag ttatttaatc
     7441 tcaaacgaaa gccgtggatc atgccggatg aagtcgacgt cgtcactgtg gttaaagtga
     7501 ttgatacgtg cccgagcggc gcgctgaaat accgtcataa ataagcgagg gtaaaatgga
     7561 aatacgcgaa ggccacaata aattttacat taatgacgaa cagggcaagc aaatcgctga
     7621 aattgtcttt gtgccgaccg gagagaattt agcgattatc gaacataccg atgtcgatga
     7681 aagcctgaaa gggcagggga ttggtaaaca gctggttgcg aaagtcgtgg aaaaaatgcg
     7741 tcgggaaaaa cgaaaaatta tcccactatg cccatttgcg aaacacgaat ttgataaaac
     7801 gcgggagtat gatgatattc gcagttgatg ggagagtaca gagtcacgat atttttcatg
     7861 ctctccgcgg agtgatgcag gagagcatct gaagggtagg gggatgcaca aagaatgggc
     7921 agagagcgcg tttttttgtc ccaagtcatc ccctttactg agcaaaaaaa agaatatctc
     7981 ctatatgaga atcatcaatc ggggttaata agttttgcgt ccccagagca ttcaatattg
     8041 ataggagtca tattatggaa ggtaaaaaca agttcaatac ttatgttgtt tcttttgatt
     8101 atccatcatc ttattcctca gtgttcttaa ggttaagatc attgat
//
LOCUS       DAJTMC010000177         8125 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.177, whole genome shotgun sequence.
ACCESSION   DAJTMC010000177 DAJTMC010000000
VERSION     DAJTMC010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8125
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.177"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(135..211)
                     /locus_tag="OO130_003570"
     tRNA            complement(135..211)
                     /locus_tag="OO130_003570"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(175..177),aa:Asp,seq:gtc)
     gene            complement(344..1075)
                     /gene="dnaQ"
                     /locus_tag="OO130_003571"
     CDS             complement(344..1075)
                     /gene="dnaQ"
                     /locus_tag="OO130_003571"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HCR5366832.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVEKKGGSCLWRA"
     gene            1140..1607
                     /gene="rnhA"
                     /locus_tag="OO130_003572"
     CDS             1140..1607
                     /gene="rnhA"
                     /locus_tag="OO130_003572"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HCR5366833.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(1604..2326)
                     /locus_tag="OO130_003573"
     CDS             complement(1604..2326)
                     /locus_tag="OO130_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR5366834.1"
                     /translation="MKPARVPQTVVAPDRWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLRETDRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            2360..3115
                     /gene="gloB"
                     /locus_tag="OO130_003574"
     CDS             2360..3115
                     /gene="gloB"
                     /locus_tag="OO130_003574"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HCR5366835.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAIAANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTASQMYQSLNKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            3187..4545
                     /gene="mltD"
                     /locus_tag="OO130_003575"
     CDS             3187..4545
                     /gene="mltD"
                     /locus_tag="OO130_003575"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HCR5366836.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRDSLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(4594..5190)
                     /locus_tag="OO130_003576"
                     /pseudo
     CDS             complement(4594..5190)
                     /locus_tag="OO130_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
     gene            complement(5221..6021)
                     /locus_tag="OO130_003577"
     CDS             complement(5221..6021)
                     /locus_tag="OO130_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCR5366837.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            6262..7176
                     /locus_tag="OO130_003578"
     CDS             6262..7176
                     /locus_tag="OO130_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5366838.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDREIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(7173..7966)
                     /gene="dkgB"
                     /locus_tag="OO130_003579"
                     /pseudo
     CDS             complement(7173..7966)
                     /gene="dkgB"
                     /locus_tag="OO130_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414743.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
BASE COUNT         2176 a         1951 c         1966 g         2032 t
ORIGIN      
        1 tggtctacca tatgtgatta acacatttac agcataacaa aaccatagac cgaagaaata
       61 gcgggattta aatgcaaaaa aaccgcaaaa ttaaaaatct tgcggttctc tgaactcatt
      121 ttcatgagtg aatttggcgg aacggacggg actcgaaccc gcgaccccct gcgtgacagg
      181 caggtattct aaccgactga actaccgctc cgcgttgtgt tccgttggga acgaggcgaa
      241 tattacggat tgcctcaccc ttcgtcaacg gtttttctca tcttttgaat cgtttgctgc
      301 aaaaatcgcc caagccgcta tttttagcgc cttttacagg tatttatgcc cgccagaggc
      361 agcttccgcc cttcttctcc accagatcaa gacgggcttc atgagctgca agctcttcat
      421 ctgtcgcaaa aacaacgcgt aacttacttg cctgacgtac aatgcgctga attgttgctt
      481 caccttgttg ctgctgtgtc tctccttcca tcgcaaaagc catcgacgtt tgaccaccgg
      541 tcatcgccag ataaacttcc gcaaggatct gtgcatcgag taatgccccg tgtagcgttc
      601 gtttactgtt atctatttcg tagcgggcac ataacgcatc gaggctgttg cgcttaccag
      661 gaaacatttt cctcgccacc gcaaggctat cggtgacctt acagaaggta ttggtcttcg
      721 gaatatcgcg cttaagcaac gaaaactcgt agtccataaa gccgatatcg aacgctgcgt
      781 tatggatcac caactccgcg ccgcggatat agtccatgaa ctcatcggct acttcggcaa
      841 acgtgggctt atcgagcaaa aattcatcgg caataccatg tacgccaaag gcttccggat
      901 ccaccagccg atcgggtttg agataaacat ggaagttatt gcccgtcagg cgacggttca
      961 ccacttcaac ggcaccaatc tcaatgatct tgtggccttc atagtgcgca ccaatctggt
     1021 tcataccggt ggtttcggta tcgagaacga tctggcgtgt aattgcagtg ctcatagcgg
     1081 tcatttatgt cagacttatc gttttacagt tcgattcaat tacaggaagt ctaccagaga
     1141 tgcttaaaca ggtagaaatt ttcaccgatg gttcgtgtct gggcaatcca ggacctgggg
     1201 gttacggcgc tattttacgc tatcgcggac gcgagaaaac ctttagcgca ggctacaccc
     1261 gcaccaccaa caaccgtatg gagttgatgg ccgctattgt cgcgctggag gcgttaaaag
     1321 aacattgcga agtcattttg agtaccgaca gccagtatgt ccgccagggt atcacccagt
     1381 ggatccataa ctggaaaaaa cgtggctgga aaaccgcaga caaaaaacca gtaaaaaatg
     1441 tcgatctctg gcaacgtctt gatgctgccc tagggcaaca tcaaatcaaa tgggaatggg
     1501 ttaaaggcca tgccggacac ccggaaaacg aacgctgtga tgaactggct cgtgctgcgg
     1561 caatgaatcc cacactggaa gatacgggct accaagtcga agtttaagct tgtggtttac
     1621 gacattgccg ggtggctcca accgcttggc gaattcgtgg cttgttttta ctctgtttca
     1681 tcgggtttag cgttaaagga atagtccgtt tccgggcaac aataagttgt aagcaaccaa
     1741 gcgcaggaat atgcgcattc aatagttttc ctccgtgttt gttccacggg agaacgtgga
     1801 aacggctggc gtgcagcact tcaaaattca acaaagagag ccagtccagt tgccgcatca
     1861 gagtaaacat ccggctgtta tagggcgagg tttttcgcaa taccggcaca agtttgcgta
     1921 atcccattaa actaatggga ttgaagccac taatcaccag ccagccatca tcaatcaata
     1981 cccgatcggt ttcacgcaat aaacgatgcg gatcggtgca ccacggcaat gtatgtgcca
     2041 gtagacaaac atcaacggat ttatcggcaa aaggaagatg aagtggatcc gcctgcacct
     2101 ggacgggcat tccttgcgca gaaacattca cttgatgaga aaccgcgcac gcttcgcaat
     2161 tgatttctgc gcttaaattg ccaatcttaa gcagatgaaa accatacatt ttagtgaacc
     2221 atgggttgag ctggcgctcc agcgccttgc gataaagctc tccccagggc aaatcgcccc
     2281 agcgatcagg agccacgaca gtttgaggga ctcttgccgg tttcatcaca accttccgtt
     2341 tcacactaag aggtaatcta tgaatcttaa cagtattccc gcctttgatg acaattacat
     2401 ctgggttttg aatgatgaag caggtcgctg cctgattgtc gatcccggag acgcagagcc
     2461 agtattaaac gccattgccg ccaataactg gcaaccggag gccatatttc tcacccacca
     2521 tcatcacgat cacgttggcg gcgtaaaaga actggtggaa aagtttccgc aaattgtggt
     2581 gtatggtcca caagagacac aagataaggg aacaacacag gtagtcaaag atggcgaaac
     2641 tgccttcgtt ttggggcatg aatttagtgt aattgccaca ccgggtcaca ctttaggaca
     2701 tatctgttac ttcagtaaac cttatctatt ttgcggcgac acgctgtttt ctggtgggtg
     2761 tggtaggttg tttgaaggga cagcatcaca aatgtatcaa tcacttaata agttaagtgc
     2821 gttacctgac gatacattgg tatgttgtgc tcatgaatat acgttatcaa atatgaagtt
     2881 tgctttgagt attcttccgc acgatttgtc cataaatgat tattatcgta aagttaagga
     2941 gttacgggca aaaaatcaaa taacactacc cgtaattctg aaaaatgagc ggcaaattaa
     3001 tgttttttta agaacggaag atattgattt aattaatgta attaatgaag aaacattatt
     3061 gcaacaacct gaagagcgtt ttgcatggtt aaggtcaaag aaagataggt tctgataaaa
     3121 ctttcttgtc atcggctccg ttcgccgtta tgatcggtcg tcttttaagc aactattgac
     3181 acacacatga aggcaaaagc gatattactc gcctctgtcc tgctcgtggg ttgccagagt
     3241 accggcaacg ttcaacagca cgcacagagc ctttctgcag ctggtcaagg ggaagcagca
     3301 aagtttacaa gtcaggcacg atggatggac gatgggacgt ctatcgcgcc agatggtgac
     3361 ttgtgggctt tcattggcga cgagctaaag atgggaattc cggaaaatga ccggattcgc
     3421 gaacagaaac agaaatattt acgcaataag agctatctcc acgatgtaac tttacgggca
     3481 gagccgtata tgtactggat agcagggcaa gttaaaaaac gtaacatgcc tatggaactg
     3541 gtactactac ccatagtgga gagcgctttt gatcctcacg caacgtctgg cgccaatgcc
     3601 gcaggcatct ggcagatcat tccgagcacg gggcgcaatt atggtttgaa acagacccgc
     3661 aattatgacg cgcgtcgcga tgttgttgct tcaacaactg ccgcgctgaa catgatgcag
     3721 cgtctgaaca agatgtttga cggcgactgg cttctgaccg tagcggctta taacagcggc
     3781 gaaggtcggg tcatgaaggc aattaaaacg aacaaagcgc gtgggaaatc cacggacttc
     3841 tggtcgttac cgttgccgca ggaaacgaag cagtacgtgc ctaaaatgct ggcattgagt
     3901 gatattctca aaaacagcaa gcgttatggc gtacgtctgc caacgaccga tgaaagccgt
     3961 gctctggcgc gtgtgcacct gagcagcccg gttgaaatgg cgaaggttgc agatatggcg
     4021 gggatttccg tcagcaagct gaagacattc aacgctggcg taaaaggctc cacgctgggc
     4081 gcaagtggcc cgcagtacgt gatggtgcca aagaagcatg cagatcaact gcgtgattct
     4141 ctcgcttcag gcgaaattgc tgctgtacag tcgacgctgg ttgccgacaa tacgccgctt
     4201 aacagccgtg tttacaccgt acgctctggc gacacgcttt caagcatcgc ttcacgtctc
     4261 ggcgtaagca ccaaagattt gcagcagtgg aacaaactgc gcggatctaa gctgaagcca
     4321 ggccaaagtt tgacgattgg tgcaggcagt agcgcacagc ggttggcaaa caacagcgat
     4381 agcattacgt atcgtgtgcg caaaggcgat tcgctttcaa gcattgctaa acgccacggc
     4441 gtgaacatca aagatgtgat gcgctggaac agtgacactg cgaatctgca accaggcgat
     4501 aagctgacgt tgtttgtgaa aaacaacaac atgccagatt cctgacaaac cagataataa
     4561 aaaaggcacc gattcccccg gtgccttttt tatttatgcc gctttatgcg cttctaccat
     4621 gatgatatca ctggtgaaag agccatcagt ctgcaaggaa aaatacgttc tcacctctgt
     4681 cgatgcgctc tgttggtaaa tgcgaatagc gtctactaac gcttctggcg tacgcattct
     4741 cacgacccat gaagaaaatt ccagcggtaa cttatctgta attaaattat caactatcag
     4801 attggcttca ttgattaacg tcaaccacgc accgctggcg tagtttcgta cgtgagaggt
     4861 atcgcgtaat gcttctaccg cctgtaacca gatgtcgcgc actgggtgac ccggagacat
     4921 tacatccatc acaatcagcc taccgccagg tttcaatatc ctattcactt ctcgcagtgc
     4981 tgcaccaaca tcatgccagt gatgggcaga ataacggctg ataacaatat caaatgcgtt
     5041 atcggcaaat ggcagacttg cggcatatcc ctggcgggtg gtgatatttt tcagttgccg
     5101 ggcttcggca gcttgtgcca caacatccag catgtgggcc gataagtcat acgccaccac
     5161 cgcgctcacg ttttgcgcag cgccaaacgt tggtctgccc tgtggcagac ctgacatacc
     5221 ttatttatca ggcttgccgg gactgaattc aacgagtagc ggattgtgat cggaagcgcg
     5281 cgtaaccagt acagaagctt cactgacgtt cagaccacgg tagaaaacaa aatcgagcgg
     5341 gcgaccaaac gcccgacggc gctgatcatc ggtaaaacgc acctggcgca gcgacatttc
     5401 ccgcgcaaag cgatataacg cgttcatcct tctacggctc caggcattga aatctcccgc
     5461 cataatgacc gggccgctgt ggtgagctat ctgatcacca ataggaagta actgcttact
     5521 atagacatcc acgcccagac tgaagttgac ggcgtgtata ttaaccacca tcaacaggcg
     5581 ggtgtcaggt aatggataga ccgtcaccag tgccgacttc gccagacgca aaatgggttc
     5641 tcgttcacgt aacgggcagc aatacactgg atgtgccgcc gaaagggtca ttacgccaga
     5701 aggatgttgt ggcagcacga aagcgggtac ctgatcggcg gcaagatagt tagcggtcgc
     5761 aaactgtact aactctggcg ttgtctgcgc ttcctgcaat aacaccaggt gtgcatcttt
     5821 gccgtagttc tttaataccg acaaccattc agcgcgttgc tgtttgtata tgttccacac
     5881 caggatccga atacgctctt cggtacttaa cggttcccca ggtggtaatg cctggccgat
     5941 gctcgcaaaa gaccccggcg gtaagatcct ttccgcaggt tgtccggcaa catagcgcat
     6001 ggcataggtg ttttttcgca cttttgactt tgaaacctct attacgtcaa cccttccgca
     6061 ctcgggaggg ttctactatt atagggattt tagctcactc tttcaacgag caattgtata
     6121 ttgttatgta agcaagtgct tacaccggtc agaattttgc tcattatgga gaacattaaa
     6181 tcgcatgaca tatcacgcat cctgatggtt taatgacaaa taaattcaaa atatctcttc
     6241 tattttgcaa caggagcaaa aatgaaagcc acgtcggaag aactcgccat ttttgtttcg
     6301 gtcgtcgaaa gcggcagctt tagccgggca gcggaacaat tagggcaagc aaactcagcg
     6361 gtaagccggg cggtgaaaaa gctggagatg aaacttggcg ttagcctgct taatcggacc
     6421 acgcgacaac ttagcctgac ggaagaaggc gagcgttatt ttcgtcgcgt acagtcaatt
     6481 ttgcaggaga tggcagcggc agaatcagaa attatggaga cgcgtaatac accgcgtgga
     6541 ctgttacgga tcgatgccgc aactccagtg gtgctgcact ttctgatgcc gttaattaag
     6601 cctttccgtg aacgctatcc ggaagtcact ttgtcactag tctcctccga aacgattatt
     6661 aatttgatcg aaagaaaagt ggatgtcgcg atacgcgctg gtacgttaac ggattccagc
     6721 ttacgtgcca ggccgttatt taacagttat cgaaaaatta tcgcctcccc cgattatatt
     6781 tcccgctacg ggaagccaga aacgatcgac gatttaaagc aacatgtttg cctgggattc
     6841 actgaacccg cttccctcaa tacctggccg atagcctgta gtgatggaca attacatgag
     6901 gtgaaatacg gtttgtcatc caatagtggg gaaacactga aacagctttg cctgagtggc
     6961 aacgggattg cgtgtttgtc cgactacatg atcgacagag aaatcgctcg cggagaattg
     7021 gtggagttaa tggcagataa agtgttgcca gtggaaatgc cattcagtgc cgtctattac
     7081 agcgaccgtg cggtaagtac gcgcatccgg gcttttatcg atttccttag cgagcatgta
     7141 aaaacagctc ccggaggagc tgtcagagag gcttaatccc attcaggagc cagaccttcc
     7201 gggctaacca ggcggtcgtt gcaatccagt gcggcgatcg cttttttatc ttcggcatca
     7261 agctgtaaat tttgtgcctt aagattactt tccaggtttt tacgtttagt agaagaagga
     7321 attactgagt aaccttcccc catagcccac gccagaatca cttgtgccgg agtcgcattg
     7381 tgtttagctg cgatacgagc aataacctca tctttcaggg ccttaccata cgccagcgtc
     7441 atataggaag taatatgtat gccgtgctgt ttagcccagg caaccacttt acggttttgc
     7501 agataaggag agagttcaat ctggttagta gcgatgtttt cagcgccaac agcagcaatc
     7561 gccttttcca tcaatgggat cgtgaagttg gaaataccga tctcacgcgt cagcccttgt
     7621 tttttggctt ccagcagcgc ctgcataacc ccttcaacag agacttcatc gtttggtgac
     7681 ggccagtgga ttagcgtcag atcaacataa tcggtacgca atttttgcag gctctctttc
     7741 agacttggga tcaatttgtc tttgctgaga ttttcaatcc agattttagt ggtgatgtag
     7801 agttcatgac gtggcacgcc actttctgca atcgcctgac ctactgcggc ttcgttatca
     7861 tagatttgtg cggtatcaat tgcgcgataa ccaagttcaa gcgccgtttt cacagatgaa
     7921 ataacaacgt agtctttcaa cctaaaccaa atgcagggat agccataata tgcctctttt
     7981 aatgctattt tcgttaactg aggtgattat gggcttcaga ctgttgagga aaaaagcgta
     8041 aaatgcagag gatttttgcg attctggcaa taatagatat acagacgaaa aaaaacctcg
     8101 agcgttaact cgaggcttct taata
//
LOCUS       DAJTMC010000178         7864 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.178, whole genome shotgun sequence.
ACCESSION   DAJTMC010000178 DAJTMC010000000
VERSION     DAJTMC010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7864
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.178"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(496..858)
                     /locus_tag="OO130_003580"
     CDS             complement(496..858)
                     /locus_tag="OO130_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HCR5366839.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
     gene            1143..1352
                     /locus_tag="OO130_003581"
     CDS             1143..1352
                     /locus_tag="OO130_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5366840.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            complement(1364..1951)
                     /gene="mtlR"
                     /locus_tag="OO130_003582"
     CDS             complement(1364..1951)
                     /gene="mtlR"
                     /locus_tag="OO130_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HCR5366841.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(1951..3099)
                     /gene="mtlD"
                     /locus_tag="OO130_003583"
     CDS             complement(1951..3099)
                     /gene="mtlD"
                     /locus_tag="OO130_003583"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCR5366842.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPSMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            complement(3329..5242)
                     /gene="mtlA"
                     /locus_tag="OO130_003584"
     CDS             complement(3329..5242)
                     /gene="mtlA"
                     /locus_tag="OO130_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HCR5366843.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLT
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            5779..6141
                     /locus_tag="OO130_003585"
     CDS             5779..6141
                     /locus_tag="OO130_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HCR5366844.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIASLSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            6144..7280
                     /locus_tag="OO130_003586"
     CDS             6144..7280
                     /locus_tag="OO130_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR5366845.1"
                     /translation="MDLLIVLTYVALAWAVFKIFRIPVNQWTLATAALGGVFLVSGLI
                     LLMNYNHPYTFTAQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQAR
                     VDRLQADLMTATHNIKTLRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFS
                     ERDIDDARQNFLAQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLE
                     QTVIRAPSNGYVTQVLIRPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGD
                     DAEVVFNALPGQVFHGKLTSILPVVPGGSYQAQGGLQSLTVVPGTDGVQGTIELDPND
                     DIDALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWMHYLYLDH"
BASE COUNT         1964 a         1909 c         1903 g         2088 t
ORIGIN      
        1 gtccattgta ttattggtga tttatgttgc tttacattaa tgacagtgat gtgtgtcgat
       61 taaatattgt tttaagcatt catcataaaa atgtatcatt cgtttcaaaa gatgtctgtg
      121 tttgtatgaa tatattattt tcgtggattc taatgatgat tattaataaa tgtcaaatat
      181 gaaagaaacc atgtctggga aaaaatttac agataattta ttgtatgaat atgatgatta
      241 aaatttcaca attaaatccg aaaaaaagag acgtaactgc ttgttaatta ataaatgata
      301 tcttctatat atttttattg tttataggtg gctatttcgg taaaaatgca ttttcagaat
      361 atcaaccagc ggtaaatcga cgtaaaggga gtgaattgtt tggtaattag gcgagtagac
      421 aggagagtcc ccgcattgac cagctaaggc aagccagatg cgggttgata tgatcgaacc
      481 agtcgtctga ggcgcttaaa acgcagtctt agagaatcca gtcatctcct gcaggcccat
      541 ttcacggccc agcgcggtca ttgggtgcac aaccaccagt ccgcgaacac ttttcttcag
      601 cttgcccata tcagcctgct cttttttggt aattgcgcgc tgaaacggca tcttcatcag
      661 cttctgtgct tctttactca gtttttggct atgaacctca cgcaggcgag caatttctgc
      721 ttccagcgtg gctttctctt tttccagctc ggcatatttg tctgcggctt caaccagcga
      781 cagatcggcc tgctggtggc ggatggcgtc caggcgatcg ctgagacgtt tgatttcgtt
      841 tttttcgact tctttcatgt gatggcttct gataaagata aatttatagc ccctagtatg
      901 cgcgaccagg tgctttgttc acaactgatg acattgtaat agttctgcaa cgtaatcatt
      961 aacatgctca aaaactacgc aattcgttga ttaattaaga aatctataga aaaaagataa
     1021 taaacagcag cggatgatta ttgaggattc agcacgtttt ccttaatgtc atctttaccg
     1081 ggaacctaaa ctaaaattgg tggccgcgca ttcccggcgc ggggtaatac ggagatatca
     1141 tcatgggcaa attaggtgaa aacgttccgc ttcttatcga taaagccgta gatttcatgg
     1201 catcaagcca ggcgttccgg gagtatctga aaaaacttcc tccccgtaac gcgattccgt
     1261 ccggaatacc cgatgaaagc gtgccgttat atctacaacg tctggagtat tatcgtcggc
     1321 tttatcggcc gaagcaggta gaggggcagt aagcagacgt tgcttacttt tgaaaggctt
     1381 tttttaagct gattttggaa atcagctcag tcagtgaaag gaccattgtt gatcgcacag
     1441 cctgttgata gcgctgaatt tgcattgcat acaaactgga gtctgctggt tcaaactgtg
     1501 gcggcggtgg taacgccgcc acgcagtgca gttcaccaaa gggtccaagg atttcgtcgt
     1561 cggtaaaggc gtactcgttg ccgtcgtgat ttagctcttc acgcaatgcc atcagcagtt
     1621 ccgcatcttc gtattcctgg cggttaatga cgcccaaccc gtaaatgagt tttaaacgca
     1681 cagaaagatc gcccagcgga ccgtcgccgt cgagtaacgg ttctacagca tacttcaccg
     1741 cgtaatcgtc tttgcggaat acctgaagca ccagaagatt taccgcctcg gtcaaaagct
     1801 cgacggcggt gatcagaaag cttcgcacgg ttttgccagc attcagacgc tcaagcacac
     1861 ggttttcaaa ggcctgggtt tgttccattg ttgcctgcat atctgacaat ctgttattcg
     1921 ggcgcagggt gtcctgcgcc tggtccacca ttattgcatt gctttataag cggttaccgc
     1981 ctcggataca acctcactgt tggcatcaag accggaaatc tgtgccagcg ccgcctgcgg
     2041 acctttgtca gcgatcagtg ctgccagttc ctgagcctgc ggatcatctt cactgcggaa
     2101 gtgcattgca ccagcaatcc cctgaatcag attcttgtgc ggaaggctgt attccagcgt
     2161 accgagcagt ggcttgatca gacggtcgcc agcactcagt ttacgcagcg gctgacggcc
     2221 tacgcgctct acatcatctt tcagatacgg gttctcaaaa cgaccgagga ttttctggat
     2281 gtacgccgca tgtttgtctg cgtcaaagcc gtagcgcttg atcagtaccg caccactttc
     2341 ttccatcgca ccttttacca ccgcgcggat tttctcgtcg agaatagcgt cacgaatggt
     2401 ctgatgaccg gccagttttc cgaggtacgc ggttatagca tgacccgtgt tcagggtgaa
     2461 gagtttacgt tcgacaaatg ccatcaggtt gtcggttaac tccatgcttg ggatgttcgg
     2521 cagtgcgcct ttgaactgcg ttttatcgac aatccattcg ctgaaagttt ctaccgtcac
     2581 ttccagcgga tcgttagttg ccgaagccga aggcggtacg atgcggtcaa cggcggaatc
     2641 gacaaagcca acgtgttctt ctacccacgc tttggcgtct tccggcaggg cgttcatcac
     2701 atggcctttc agctgcgtgg taccgcgtac catgttttca caggcgatga tgttcagcgg
     2761 ggattcatta ccttgttctt tgcgtttcac cagccctttg gcgatagccg gagcaatacg
     2821 ttccagcaca accgggccaa cggcggtagt gactaaatcg acctgagcaa tcagatcaac
     2881 gacatcatca ccaatgctgc tgacagcatt gacgccggaa acggtatcta cctgctcggt
     2941 ttcaccaacc acatgaacct gatagctatg acgggcattc agggcatcaa gtaccacctg
     3001 attgacatcg gcaaacgtca gttggatacc cgcgtctgcc agcagtttac cgataaagcc
     3061 acgaccgata ttacctgcgc caaaatgtaa tgctttcata gtattaacct tcatcaatgt
     3121 ttttacccga gagggctggg gtgaggcttt tccctcaccc aaaccctctc cgtgaggaga
     3181 ggggcagcgc cggatgcggc gtaagcgcct tatccggcct acaagaacgt gcaaattcaa
     3241 caaatcgtga tgcatcctgt aggcctgata agcgtagcgc atcaggcaat taaccccacc
     3301 ttctccatgt ggagagggtg ggattggatt acttacgacc tgccaacagt tccagcactt
     3361 catccacgct ggtggtgtgt gccagacgct cgatgacgga ctcatcatcc agtgcattgg
     3421 tcaggctggt gataacctga atgtgctcgt tgttacgggc tgcaatacca atcaccagac
     3481 gggcaatgtc atcttcctct tcaccgaagc gcacgccttc cgggtactgg cagaacacca
     3541 cgcccgtttt cagtacgcga tctttcgctt caaccgtacc gtgtggcacc gcgatagact
     3601 cacccagata agtcggggtc agtttttcac gatccagcat cgcctgaacg tattccggct
     3661 caacgtaacc gcctttcacc agctgctcgc cagcaaaacg aatcgcttct tctttggttg
     3721 ctgctttgcg accgaggaag atgttctccg cgcccagttt gaacaggtta gcactggaat
     3781 cgtcaaagct gtctttcaga ctgtctttca ctttcacttc gttttcagta tggcgctggg
     3841 cagcaaccag acgttcggtt aggctggtgt acaggccgct gtcgaggaag ttggtcagcg
     3901 aaatatgctg tgcctgcgga acctggcgca tagcgcgttc ggtcaggtca cggtgagtga
     3961 tgacgaggtc cacatctggc ggcaggttgt tgatcgcgct gttagtgacc gaaatctgcg
     4021 acagacctgc atcctgaatt ttcttacgca gcacgcctgc gcccatcgca cttgaaccca
     4081 taccggcatc acaggcaacg atgattttac gtacgtggct caggtcgtta gtcacatcgc
     4141 ccgcagacag cggagatgtg cctttagact cagctttcat gtcctgcata cgacgagttg
     4201 ctgcttcaat atcatcttct tctttcactt tgctggtttt cagcaaaata gcagagacaa
     4261 cgaaggagac agccatcgcc gcacacacac ccgcgatgtt agcgaaataa gccccttttg
     4321 gtgtcatcgc cagtacagca aggatagaac ccggagatgc cggagaaacc agaccaccgc
     4381 ccaggatagt cagcgtgaac acgccagtca taccgccgag gatgactgcg aggatcagac
     4441 gcggattcat cagcacatac gggaagtaga tttcgtggat accacccagg aagtggatga
     4501 ttgccgcacc gcccgcagac tgtttagcgc taccacgacc aaagaacatg tacgccagca
     4561 gcacgcccat acctggacct gggttagctt caatcaggaa gaagattgat ttacccagtt
     4621 catgggactg ctgaataccc agcggcgaga agataccgtg gttaatggcg ttgttgagga
     4681 acaggatttt cgccggctca acaaagatag acgccagcgg cagcatgtca tggacaacca
     4741 tgaagttaac gcccgcagcc agcattttgg acagggcttc aacaatcggg ccaatgccga
     4801 ggaatgccag aatagcgagg atcatcccga tgatgcctgc ggagaagtta ttcaccagca
     4861 tctcaaaacc ggatttgatc ttaccgtcta cccagcggtc gaagtgctta atgcaccagc
     4921 cgcccagcgg acctgcaatc atagaaccga ggaacatcgg catgtctgcg ccgacgataa
     4981 cgcccatggt ggtgatggca ccgactacgc cgccacgttc gccgcctacc agcttaccac
     5041 cggtataacc gatcagcagc ggcaggagat aagtgatcat cggcccgacc agcttcgcca
     5101 gcgtctcgtt cgttaaccac cctgttggaa taaataacgc ggtgatgata ccccacgcga
     5161 taaacgcgcc gatatttggc atcaccatgt tgctgaggaa acgaccaaag ctttgcactt
     5221 tgatcttaat atcggatgac ataaaaacac cccttcttat gtttgctgtc gcgcaggctg
     5281 gaagcccgag gttttttgtt aatgtggcgg cagaggtagc cggaccttgt agatactgcg
     5341 aaatctggca ctgaatcggt taactgtcca gtcgacggcc ttttgtgtga tattaatcac
     5401 ataaatatag gaggtagaga ggtaattgac gtgacgttca tcacaaaacg cttgtgtaaa
     5461 aaagcaacaa ggcggcgatt ttcgctaaaa atggcattta aatgtgagta gtgtcacatt
     5521 tttgttttga tggtttgttg aatctttgtg atctgaatca caagatattt tcttacggcg
     5581 ttcacctgat gtgatctaca gcatgttatg ccttcgtcat acaatcgtta tgtaactaag
     5641 caactcatca cctctaaaaa ttattctttg gtgttatggc gagaatcgcg aacccggatt
     5701 attgtcaaaa tatcagcctg actcccttct ggctgccagc ccaaaaacaa cactgataaa
     5761 cgtgaatccg aggcagatat gtttctaaac tatttcgcgt tgggagtgct gatcttcgtc
     5821 tttctggtga ttttttatgg aatcatcgcg atacatgaca tcccttatct gattgccaaa
     5881 aagcgcaatc acccccatgc cgacgctatt catacggcgg gctgggtgag cctgtttact
     5941 ctgcatgtta tctggccgtt tctgtggatc tgggcgacgc tctatcaacc ggagcgtggc
     6001 tggggtatgc agtcacatgt tgcgtcgcag gagaaagcga ctgacccgga aatcgcctca
     6061 ctttctgacc gaatttcccg gctggagcat caactcgccg ccgagaaaaa gactgactat
     6121 tccacgttcc cggagatcta attatggatc tattgattgt tttaacttac gtggcgctgg
     6181 cgtgggcggt ctttaaaatc ttccgcattc cggtaaatca gtggacgctg gcgacggcgg
     6241 cgctgggagg cgtgtttctg gtgagtggtt tgattttgtt gatgaactac aaccaccctt
     6301 acacttttac cgcgcaaaag gcagtgatag cgatccctat cacgccacag gtgacgggaa
     6361 ttgttactga agtcactgac aagaataatc agcttattca aaagggcgag gtgcttttta
     6421 agctcgaccc ggttcgttac caggcgcgag ttgacagact tcaggctgac ctgatgacgg
     6481 cgacgcataa tataaagacg ctgcgtgcgc agctcactga agcgcaggcc aacaccaccc
     6541 aggtttcagc ggagcgcgac cgtctgttta aaaattatca acgttacttg aaaggcagcc
     6601 aggcggcggt gaatccgttc tcggaacgtg acatcgacga tgcgcggcaa aatttcctcg
     6661 cgcaggatgc gctggtgaaa ggctcggtgg cggagcaggc gcagatccag agccagctcg
     6721 acagtatggt taacggcgag caatcgcaga ttgtgagctt aagagcgcaa cttactgaag
     6781 caaaatataa ccttgagcag actgtcattc gcgcgccgag caatggctac gttactcagg
     6841 tactgatccg cccaggtaca tacgcagctg ccttgccgct gcgtccggtg atggtcttca
     6901 tccccgagca aaaacggcaa attgtcgccc aatttcggca aaactcgctg ttacgtctga
     6961 aacccggcga tgatgcggaa gtggtgttta acgcgctacc tgggcaggtg tttcacggca
     7021 aactgactag tattttacct gtcgtgccag gcggttctta tcaggcgcag gggggattgc
     7081 aatcattaac ggtcgtgccc ggcacggacg gtgtgcaggg aaccattgaa ctggacccta
     7141 acgatgatat cgatgcctta cccgacggca tctacgccca ggtggcggtt tactccgacc
     7201 atttcagcca tgtttcggtg atgcggaaag tgctgctaag aatgaccagc tggatgcatt
     7261 atctttattt ggatcattga gggggagggg ggagattatg gaactttgct ccttgtaagg
     7321 actaattatt tttgcatatt acttcattag ctgcgatgat ttttctttaa tattcattat
     7381 gttaataagt tctggatgag tgtgctctca ttggattttt tatccgtctt gttctgccgc
     7441 aggatgagac tatacatcgt cgagatcgca atgatgatat gagttgttcc tcctgcgacg
     7501 gctctaatca aaaaagagca ggagtgcttt tccatctatt gcgatgatgg ctttattatc
     7561 tgaaggatga cagtcacatt taaaaaatat ttaatctata gatcacaatg tagggtcatg
     7621 gaatatatgt atacaactat tttcttcttc acttttaatt atcgtattca ttataaaact
     7681 aagttaaaaa aactcctgga gagtgagtta ttaatatatt tttaaataaa attaagttat
     7741 cattcatcga tgcattttat agaatcatca atggccataa tagtactatt aactctcaat
     7801 tttatatttt tatcatcact acaaatatta actgaattta tatcaatgat ttttttattc
     7861 ttat
//
LOCUS       DAJTMC010000179         7818 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.179, whole genome shotgun sequence.
ACCESSION   DAJTMC010000179 DAJTMC010000000
VERSION     DAJTMC010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7818
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.179"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            268..411
                     /locus_tag="OO130_003587"
     CDS             268..411
                     /locus_tag="OO130_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HCR5366846.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            424..633
                     /gene="cspE"
                     /locus_tag="OO130_003588"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             424..633
                     /gene="cspE"
                     /locus_tag="OO130_003588"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCR5366847.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            799..1608
                     /gene="rlmA"
                     /locus_tag="OO130_003589"
     CDS             799..1608
                     /gene="rlmA"
                     /locus_tag="OO130_003589"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HCR5366848.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDDKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTFCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(1605..2171)
                     /gene="mntP"
                     /locus_tag="OO130_003590"
     CDS             complement(1605..2171)
                     /gene="mntP"
                     /locus_tag="OO130_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HCR5366849.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
     gene            complement(2599..3057)
                     /locus_tag="OO130_003591"
     CDS             complement(2599..3057)
                     /locus_tag="OO130_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 domain-containing protein"
                     /protein_id="HCR5366850.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDNLERIYKLIVLTQ"
     gene            complement(3112..3963)
                     /gene="manZ"
                     /locus_tag="OO130_003592"
     CDS             complement(3112..3963)
                     /gene="manZ"
                     /locus_tag="OO130_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HCR5366851.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            complement(3976..4776)
                     /gene="manY"
                     /locus_tag="OO130_003593"
     CDS             complement(3976..4776)
                     /gene="manY"
                     /locus_tag="OO130_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HCR5366852.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            complement(4839..5810)
                     /gene="manX"
                     /locus_tag="OO130_003594"
     CDS             complement(4839..5810)
                     /gene="manX"
                     /locus_tag="OO130_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HCR5366853.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            6273..>7818
                     /gene="yoaE"
                     /locus_tag="OO130_003595"
     CDS             6273..>7818
                     /gene="yoaE"
                     /locus_tag="OO130_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HCR5366854.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDVDNPMPIPE
                     GAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLFPV
                     CRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRARGS
                     FVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQQA
                     LDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIVKE
                     QPAHDED"
BASE COUNT         1987 a         1913 c         1940 g         1978 t
ORIGIN      
        1 gcactatgta ataacttctc ccactggcct ggaacaactg aacttattga actatgttag
       61 aaaatacgcc agtttaagta tctgcctgaa ctggcaaggt taagcacaat gatatatcgg
      121 cgcgtattcc gttgcataag tgtgcaaaaa aagtggaaga cgtatcgaga tttgtgcgtc
      181 tgatcgagac atgtttaaaa atggcttgcc ataattaacg ttgtatgtga taacagattt
      241 cgggttaaac gaggtacagt tctgtttatg tgtggcattt tcagtaaaga agtcctgagt
      301 aaacacgttg atgttgaata ccgcttctct gccgagcctt atattggtgc ctcatgcagt
      361 aatgtgtcag ttttatctat gttatgcctg cgggcgaaga aaacaatcta aggaattttt
      421 caaatggcaa agattaaagg tcaggttaag tggttcaacg agtctaaagg ttttggcttc
      481 attactccgg ctgatggcag caaagacgtg ttcgtacact tctccgctat ccagggtaat
      541 ggcttcaaaa ctctggctga aggtcagaac gttgagttcg aaattcagga cggccagaaa
      601 ggtccggcag ctgttaacgt aacagctatc tgatcgaatc cactgatctg aagtgtgaat
      661 acgcttcaat ctcgctataa agcctcgtcg aatgcgaggc tttttactat gctttatctt
      721 cgctcctggc gttcggatat ttgcccgccg cgtgattcgc gttacacttg cggcctttag
      781 tatcctgccg gagttgtcat gtctttttcc tgtccacttt gccatcagcc tctttcgcgt
      841 gaaaaaaaca gctatatctg tccccagcga catcagtttg atatggcgaa agaagggtat
      901 gtcaatctgc tgcccgttca gcataaacgg tctcgtgatc cgggcgacag cgcggaaatg
      961 atgcaagcac gccgcgcatt cttagatgcc ggacattatc agccgctgcg tgatgcaatt
     1021 gtcgcccaac tgagggaacg gcttgatgat aaggccacgg cggtgctgga tattggctgt
     1081 ggtgaagggt attacacaca cgcatttgcc gatgcgttgc ccgaaatcac cacgtttggt
     1141 ctggatgttt cgaaggtagc gataaaagcg gcggcgaaac gctatccgca ggtcactttt
     1201 tgtgtcgctt ccagccaccg tttgccgttt tccgatacca gtatggacgc cataatacgt
     1261 atttacgcgc cgtgtaaagc agaagaatta gcacgagtag tgaagcccgg cggctgggtc
     1321 attactgcca cgccgggacc gcgacatttg atggagctga aggggctgat ttacaatgaa
     1381 gtacatcttc atgcacctca tgcagaacaa ctggaaggtt ttacattaca gcagagtgcg
     1441 gagttgtgtt atccgatgcg tcttcgcggt gatgaagccg tcgcattatt gcagatgacg
     1501 ccgtttgcct ggcgtgcgaa gccagaagtc tggcaaacac tggcagcaaa agaagtgttc
     1561 gactgccaga cggactttaa tattcacctc tggcagcgtt cttattaacc gtggaagtgc
     1621 gtccagagga tctggacgcc gatgccgatc agtaccagcc cgccgagaat ttccgctttt
     1681 ttcccaataa ttgagccgat aaagcgacca accatcatcc ctaatgttga cataatcaag
     1741 gttgcacaac caatggccaa tgcggtcgcg ataatgttga cctgcaggaa agcaagacca
     1801 acacccacag ccatggcatc caggctggtg gcaatcgcgg tggttaccag tagccagaaa
     1861 ccgtgtcgac ggcgcggctc ttcatcttca tcatctgcgc cacgaaaacc ctcaataatc
     1921 attcgcccgc cgaggaatat cagcagcaca aacgcaatcc agtggttcca ttcaaggaca
     1981 aaccggctgg ctaacatgcc cattccccag ccgatcagcg gcgtcagggt ttcgacggca
     2041 ccaaaaataa ggccggttcg caatgcttca gaaaatttcg gtttatggag ggtggcacct
     2101 ttaccgattg atgcagcaaa tgcatccatc gacataccaa acgcaagaag aacagtagca
     2161 gtgatattca tgacaatgtc ctgaccgggg tatccataaa acacatcatc gcccccagca
     2221 aacatgcata catgaacaac aagccgtgat ggttttcgcg ttcatgcagg catagcgatt
     2281 gatgtgcata tggtctcgcc tgactgaaag tattcagtcc gtacgtgcca cgttttgcaa
     2341 cgagtatgtt gacacgtaca tttccggaat ctggaaatcg gctactcccc aaatgagggc
     2401 gcaaccttaa catattttga gaatataaaa caactgttgg aatatattat ttcattgctt
     2461 tattgataac gaattcattt agatttatat atcaatgaaa cgttaaaata aataacaaat
     2521 aagggaagag gtaatatagc ctaagctatg tctgattatt aaaaatacac taaacatagc
     2581 tttggctaaa ttcataactt attgagttaa aacgataagc ttataaattc tttccagatt
     2641 gtcgatattt cgaacgcgga ttaacagccg acgctgttct aattgcatca ccaacacgcc
     2701 atcttccgac aagttcatcg ctttgattcg gctatattca atccagacat tggcgaagaa
     2761 aaaacctttt tgtttaaaga tgatcttcgg aacgcggatc cagaatatat aaaaacccat
     2821 cagagccagt gcgcttaata accatgtcgt tattaatgca ccatgattcg tgacgttgtt
     2881 atagataaga atgacaatca atccgacgaa gataacgcta tcgatgcgac cacgccggag
     2941 caaaggaatt gccagcaggg tggggccgtt acggcggggc atgatgaact gatcgtagat
     3001 cgcgaaggcc aggagtgcgg cgatgaaaag aatcagtacc aggtccgtga ttgtcattaa
     3061 tcctccagat aaaaaaacgg ggccaaaagg ccccggtagt gtacaacagt cttacagtcc
     3121 cagcaggccg caagcgtaac cagcgatacc gatgacgaag aagccaacga tgatccacag
     3181 cgggttaact tttttgcgca gtagccacat acaagcaaag gtcagcagca gtggtaccag
     3241 gcctggcatt aactggtcca gaatagtctg gacagtagta acgtgttctt tgcccgtctg
     3301 gtcagtaatg cgagagacaa ccagcgggat gttgacatgt gtccacttgt taaccaatgc
     3361 ccccatgaca aacaggccga ggatagacgc cccttccgtc agtttttgca ggaagccacc
     3421 acccatatct ttaacgatat cgataccttt ggagtaacca tacgctacgc cgtagtaacg
     3481 ggttgccaga cgcaccaggt taaacaggat gaagaacagc agcggaccta acaggctgcc
     3541 gctcatcgcg ataccggcac ccagtgctgc aaataccgga cgtacggttc cccagaagat
     3601 cgggtcgcct acaccagcca gcggccccat caaaccgact ttgataccgt tgatagcacc
     3661 gtcgtcgatc tctgcgccat tagcacgctg ttcttccagc gccagggtta cgccgagaat
     3721 cggcgcagcc acgaacggct gggtgttaaa gaactccagg tgacggcgaa tagcttgttt
     3781 acgagcttcg ttgttctcag ggtagaggcg acgaattgcc ggtaccatag agaagcagaa
     3841 acccagtgcc tgcatacgtt cgaagttcca tgaaccctgg aagaggttag aacgcaggaa
     3901 gacgccacga atatcacttt gagtgagttt tttctcggtg gtagtttgag ttgtatcaac
     3961 catttcgctc acctgttagt ccagttcgtt atcgagatcg ttgttaccag cagcctgagc
     4021 aggcgcaccg gctacgcggt tgtatttcgg gctaagttgg atgtagagca ctgccataac
     4081 agtaccgatc acacccagag caaccaggtt aaagttggtg aatgctgcgg ttacgaagcc
     4141 gaggtagaag aacggcatca ggtagccagc acgcatcatg ttgataacca tcgcataacc
     4201 aaccaccacg atcatgccac cggcgatatt cagaccattg gtcaccactt ccggaatcgc
     4261 attcagcatg ttctgtactt cgctggtacc aacagacagc gcaacgatga cggccggaat
     4321 agccacacgc attgcttgca ggaacagaga agaaacgtgg atccaggaaa tcgctgtcag
     4381 gttgccgtta tcagcagcct tatccgcagc gtgctggaaa gcaacggtaa tagtacgaac
     4441 gatgatggtc agtacctggc ccgcagcggc cagagggatt gccagtgcga tacctgcacc
     4501 aatgctctga tgacctgcga taaccagaat ggtagaaatg atagaagcca gagcggcgtc
     4561 aggcgcaact gcagcaccga tgttcatcca gcccagcgcg atcatttcca gcgtaccacc
     4621 gataataata ccggttttca tatccccaag aacgataccc accagggtac acgcgattag
     4681 cggacggtga aactgaaatt catcgaggat tgatcccata cctgcgatac aggctacgat
     4741 aaataccagc acaatttgaa gagtggtaat ctccattgta cttctcctgt ttacgtaaga
     4801 cttaagtgtg aaaactgagt gataatcaac acaatacgtt acttatcgat tttgctgatc
     4861 agatccatca ttttcagttt cggatcggtg gaaaccttac ggacttccag ctcaataccg
     4921 cgcgcattca gtttcttgaa cgcctcgata tctttttcat caaccgaaac cgcgttattc
     4981 acctgggttt taccctgacg gaatgccata ccaccgacgt taacagaggt gattttcacg
     5041 ccgccttcaa cgagacgctc tacatctgtt gggttggtaa ataacagcat tacgcgttcg
     5101 ccagcatatt tcgggttgtt gtagacgcga atcattttgg caacatcaac tacgtgtgct
     5161 gttacgcccg gaggtgcaac ctgggtgagc agtgtcttac gaacggtatc cgcagccact
     5221 tcatcactaa caacaataat acgggagaca ttggtttctt tggtccagcg ggtggcgacc
     5281 tgaccgtgaa tcagacggtc gtcgatacgc gcaaggccaa taaccatgta gtcgtttggc
     5341 cccattggtt ttgccggagt tggagccgct tttggtgctg cggcagcggg tgctggcgcg
     5401 gctttttcaa ccggtttggc tttcagtgct ttcacacctt cacggcctgt ttctactgcc
     5461 agcgcaacca gttcatcaaa gcttgggtca tcatcacgag ccattaacgt ttccacgagc
     5521 attggaatgt taacgcctgc aatgacttca taatgctctt tgtcgacgac aatgcggctg
     5581 gcagcattga acgggctgcc tccccatgta tcaacgagaa acagcacgcc tttagtggtg
     5641 tcgagttttg ccaactgagc gttgtacttt tcaatcagcg tttcggcatt ttcacctgga
     5701 acgaaatcga tccagccgac gttttcctgc tcgcctaaca gcatttctgc cgttttaagc
     5761 aactgctctg cagcccaacc atgtgtgcct ataacaatag caatggtcac ttgctacctc
     5821 ctttattatc gttaacacct caacgtgcca gatgtatttt tgaatcgtcg tccacaatcg
     5881 aatcgattca gataagggct acaaccaaaa acccctgcgt ttcgcgattt attttagata
     5941 tcgaaaaaat tattttatgt gatgaagatc cgtaatttaa ctttcgatta gcagaaattt
     6001 cgaaaggtaa aatatccttg tcacattcgt ttgcaaagga aggtaaatct ttgccaaatc
     6061 agaggcgtct ctgatatgtt taactcccgt ttaacaacca tggagtatag ggcagtagcc
     6121 cgcagtatgg atcgtcaccg acgtcatttc agcatcaggc cttttaacgc ctgcctttct
     6181 ggcactctat gccgcacctt tcgtttgcat tttgtcgtta cgcctgcatt atttctggcg
     6241 tcgaatagct attccttaag caggagcttg tcatggaatt cttaatggac ccctcaattt
     6301 gggcggggct actcacgctt gttgttctcg aaattgtgct gggtatcgat aacctggtct
     6361 tcatcgccat tcttgctgac aaactgccgc caaaacaacg cgataaagcg cgtttgctgg
     6421 ggttatcact ggcgctgatt atgcgtctgg ggctgctgtc gctgatttca tggatggtca
     6481 cgctgaccaa accgctattt accgtcatgg atttctcctt ctccggacgc gacctgatta
     6541 tgttgttcgg ggggatattc ttgctgttca aagcaacaac cgaactgcat gaacggctgg
     6601 aaaaccgcga tcatgattcc ggccacggta aaggctacgc cagtttctgg gtggtcgtca
     6661 cacagattgt catccttgac gccgtcttct cgttggatgc ggtaattact gcagtaggga
     6721 tggttaacca tctgccggtg atgatggcgg cggtagtgat tgcgatggcg gttatgttgc
     6781 tggcatccaa accgctgacg cgattcgtta accagcaccc cacggtggtg gtgctctgtc
     6841 tgagcttcct gttaatgatt ggtcttagtc tggtggcaga aggtttcggt ttccacattc
     6901 cgaaaggtta cctgtatgcc gcgattggct tctcgatcat catcgaagtg tttaaccaga
     6961 ttgcgcgtcg caactttatt cgccaccagt cgactttgcc gctgcgagcg cgtactgccg
     7021 atgccatcct gcgtttgatg ggcgggaaac gtcaggccaa tgttcagcac gatgtcgata
     7081 acccgatgcc gatcccggaa ggtgcatttg ccgaagaaga acgttacatg attaacggcg
     7141 tactgacgct ggcgtcgcgt tctctgcgcg ggatcatgac gccgcgcggt gaaataagct
     7201 gggttgacgc taatctcggg gtcgatgaaa tccgcgagca actgctctct tcaccgcaca
     7261 gtctgttccc ggtatgtcgc ggtgaactgg atgaaatcat cggtattgta cgtgctaaag
     7321 aactgctggt ggcgctggaa gagggcgttg atgtggcggc gattgcttcg gcgtctccgg
     7381 cgattatcgt cccggaaacc ctcgatccga tcaacctgtt gggcgtgctg cgtcgtgctc
     7441 gcgggagctt tgttatcgtg accaacgagt ttggtgtggt acaaggtctg gtcacgccgc
     7501 tggatgtgct ggaagccatt gcgggtgaat tcccggacgc tgacgaaacg ccggaaatca
     7561 ttactgatgg tgacggctgg ctggtaaaag gcggtacaga tttgcatgcc ttgcagcagg
     7621 cgcttgatgt tgagcacctt gccgatgacg atgatatcgc gacggtcgcg ggcctcgtga
     7681 tctcggcaaa tggtcacatt ccccgtgtgg gcgatgtgat tgatgtaggg ccactgcata
     7741 tcaccatcat tgaagccaat gattatcgtg ttgatctggt tcgcattgtt aaagagcaac
     7801 cggcgcacga tgaagatg
//
LOCUS       DAJTMC010000180         7756 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.180, whole genome shotgun sequence.
ACCESSION   DAJTMC010000180 DAJTMC010000000
VERSION     DAJTMC010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7756
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.180"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            <1..588
                     /locus_tag="OO130_003596"
     CDS             <1..588
                     /locus_tag="OO130_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 family protein"
                     /protein_id="HCR5366855.1"
                     /translation="GDNKDATPIEQCTFDVETKNGLPSTVQLFYEESKDNEPLANIHF
                     QAIGSGFLTFVNACQEHDDNSLKLFASLLISLSYSSAYADLSETVYINENNESYLKAQ
                     FEKLSQRDMKKYLGEMKRLADGGEMNFDGYLDKMSHLVNEGTLDPDILSKMRDAAPQL
                     ISFAKSFDPTSKEEIKILTDTSKLIYDLFGVKSER"
     gene            951..1988
                     /gene="dusA"
                     /locus_tag="OO130_003597"
     CDS             951..1988
                     /gene="dusA"
                     /locus_tag="OO130_003597"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HCR5366856.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECGMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDAYPV
                     AVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            2122..2364
                     /gene="pspG"
                     /locus_tag="OO130_003598"
     CDS             2122..2364
                     /gene="pspG"
                     /locus_tag="OO130_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HCR5366857.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKVPKYQRYDRWRY"
     gene            complement(2529..3512)
                     /locus_tag="OO130_003599"
     CDS             complement(2529..3512)
                     /locus_tag="OO130_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HCR5366858.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGSGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            3595..5010
                     /gene="dnaB"
                     /locus_tag="OO130_003600"
     CDS             3595..5010
                     /gene="dnaB"
                     /locus_tag="OO130_003600"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCR5366859.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            5063..6142
                     /gene="alr"
                     /locus_tag="OO130_003601"
     CDS             5063..6142
                     /gene="alr"
                     /locus_tag="OO130_003601"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HCR5366860.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAGAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            6395..7588
                     /gene="tyrB"
                     /locus_tag="OO130_003602"
     CDS             6395..7588
                     /gene="tyrB"
                     /locus_tag="OO130_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HCR5366861.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNLGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
BASE COUNT         1880 a         1854 c         2028 g         1994 t
ORIGIN      
        1 ggggataata aagatgcgac tcccattgaa caatgtactt ttgatgtaga aactaagaat
       61 ggccttccct ccacggttca gctattttat gaggaatcta aggataatga acctttagcg
      121 aatatacatt ttcaagcaat aggttctggt tttttaacgt ttgttaatgc ctgccaggaa
      181 catgatgaca acagcttaaa attatttgct tcgctgttaa tttcactttc atattctagt
      241 gcctacgcag atttatcaga aacagtgtat attaatgaaa ataatgagag ctacctgaaa
      301 gctcagtttg aaaaattatc tcaacgtgat atgaagaagt acctgggaga gatgaagcgt
      361 ctggctgatg ggggagaaat gaattttgat ggctatctgg ataagatgtc acatctggtg
      421 aatgaaggga cgctcgatcc tgatatttta agcaaaatgc gagatgctgc accacaatta
      481 attagcttcg cgaagtcgtt tgacccaacc tcaaaggaag agattaaaat acttacagac
      541 acttctaaat taatttatga tttgttcggg gttaaatcgg agagataata tgtgaagttc
      601 ttcgatagta tggaaggcat tatataaaag gacccaatat ttattgggtc cttttttctc
      661 tatcaatgct attagcaggg agatatatca ccaaagttta atgtgtgatt ttttatttat
      721 cgtcgaacct ggattgttta tcattggcct taacaaagtt aacggctaat aagattattt
      781 ccatcacttc gtgagagctt catgccttga gaggatctca attttctttt gcaatgagac
      841 aggcgcttcc tgttgttatg gtatagtacc ccgctattga gcctcctgaa tagtgatgct
      901 gaataacata accccatgat atatcgataa aataatctct acatttgaaa atgcacggta
      961 attctgaaat gcaaaaaatc aaccaaacca gcgcaatgcc tgaaaaaact gacgttcact
     1021 ggagtggtcg gtttagcgtt gcaccaatgc tcgactggac ggacagacat tgccgctatt
     1081 tcttgcgtct gctttcccgc aatacgttgc tgtataccga aatggtgacc acaggggcga
     1141 ttattcacgg taaaggtgat tacctggcgt acagtgaaga agaacatccg gtagcgttgc
     1201 aactcggcgg tagcgatccg gcggcgctgg cacagtgtgc gaagctggca gaagcgcgtg
     1261 gatatgatga gatcaacctg aatgtcggct gcccgtctga ccgggtgcag aacggcatgt
     1321 ttggtgcgtg tctgatgggt aatgcgcagc tggttgccga ttgcgtgaaa gcgatgcgcg
     1381 atgtggtgtc gattccggtg acggtgaaaa cgcgtattgg catcgatgac caggacagct
     1441 atgaatttct ctgcgatttc atcaacaccg tttccggcaa aggcgagtgt gggatgttca
     1501 tcatccatgc acgtaaagcc tggctttcgg ggttaagccc gaaagaaaac cgtgaaatcc
     1561 cgccgctcga ttatccgcgt gtgtatcaac tgaagcgtga ctttccgcat ctgacaatgt
     1621 cgattaacgg tggtatcaag tcgctggaag aggccaaagc acacctgcaa catatggatg
     1681 gcgtgatggt cgggcgcgag gcgtatcaga atccgggtat tctggcggcg gtagaccggg
     1741 agatctttgg ttcctcggat accgatgcct atccggtggc ggtagtgcgc gccatgtatc
     1801 cgtacattga gcgtgaactc agccagggga cgtatctcgg ccatattacc cggcatatgt
     1861 tgggcttgtt ccagggtatt cctggcgcgc ggcagtggcg gcgttattta agtgaaaatg
     1921 cccataaagc gggtgcagac attaatgtgc tggaacacgc gctcaaactg gtggcggata
     1981 agcgttaact tttcaccaaa aagtagtcaa attcaccacg ccctgcgcac cgtcgcgggg
     2041 cgttttgctg ttaaatcaat agattatttt tggcatgatt cttgtaatgc cagcaagaga
     2101 tttcatattt gggagagcat catgctggaa ctactttttg tgattggctt ttttgtcatg
     2161 ctgatggtca ccggcgtttc gttgctgggc attatcgccg cgctggttgt ggcgacggcc
     2221 attatgttcc tcggcggtat gctggcattg atgattaagt tgctgccgtg gttactactg
     2281 gcgattgcgg tggtgtgggt tattaaggcg attaaagcac caaaagtgcc taaatatcag
     2341 cgttatgacc gctggcgtta ctaagggatt gtgcggatga tcacaaccta aggttttatc
     2401 cttagaacaa aataggaatt gataatcaaa tctgtcacta ttgcgcctct aacagattca
     2461 tcgtgctgta ccctacatac agccgactat aaaaagaaag ggcttcccag gtggaagccc
     2521 tatttctttt atggaatcag caggctggaa ccttgcgtcg cccggctttc cagaatctca
     2581 tgcgcacgct gcgcatcctt cagcggatat ttctgctgct cggcgacatc gaccttaatc
     2641 acaccgctgg caatcaaaga gaacagttca ttactggcct cggttaattc ctcccgtgtg
     2701 gtgatatagc cttgcaggga agggcgtgtc acatacaacg agcctttttg attgagaatg
     2761 cctaagttca caccggtaac cgcacctgat gagttgccaa aactgaccat taagccgcgg
     2821 cgttgcaggc aatccagcga ccgttcccag gtgtctctgc ccacggaatc gtacactacg
     2881 cgcactttct taccgccggt gatctctttc aatcgctcga ccagattctc ttcacgatag
     2941 ttaataacct gccacgcgcc ggcttttagc gcgctctgcg ctttttgcgc ggttcctacg
     3001 gtgccgataa gtttcgcgcc cagagctttt gcccactggc aggcaatcaa gccaacgccg
     3061 ccagccgctg cgtggaacag gaactgttca tcgggtttaa tttcataggt tttgcgcagc
     3121 agataataaa ccgttaagcc tttcaggaaa gatgctgcag cttgctcaaa agaaatagcc
     3181 gcaggcagaa tcgccgcttt atccgcatta atgttatgca cagagctgta agcgcctaac
     3241 gccgactgcg catagactac acgatcgcct gccttaatat gctttacacc actgccgact
     3301 ttactcacga tgcctgccgc ttcggtacct gatccgctgg gtagtgatgg cggcgggtaa
     3361 aggccgctgc ggatatacgt gtcgataaaa ttgatgccga tggctttatt ttcgacctgg
     3421 atttcattct ctgccggatc ggcaggagtg aactctacgg cttgaagtac ttccgggcca
     3481 ccgtgcttgt gaaattcaat tcgtgttgcc atgtgtcctc cagaacgtat cgtcagggtc
     3541 tgcttcatat ggtaaagttt cgacccattc tttatctcgg taactccatt cactatggca
     3601 ggaaataaac ccttcaacaa acagcaggct gaaccccgcg aacgcgatcc acaagttgcc
     3661 gggctgaaag tgcctccgca ctcgatcgaa gcggagcagt cggtgttggg cggtttaatg
     3721 ctggataacg aacgctggga cgatgtagcc gagcgtgtgg tggcagacga tttttacacc
     3781 cgcccacacc gccatatctt tactgaaatg gcgcgtttgc aggaaagcgg tagtcctatc
     3841 gatctgatta cccttgcgga atcgctggaa cgccaggggc aactcgatag cgtcggtggt
     3901 tttgcttatc tggcagagct gtcaaaaaat acgccaagtg cggcgaacat cagtgcttat
     3961 gctgacatcg tgcgtgaacg tgccgttgtc cgtgagatga tctcggttgc gaatgagatt
     4021 gctgaagccg gttttgatcc gcaggggcgt accagcgaag atctgctgga ccttgctgaa
     4081 tcccgcgtct ttaaaattgc cgaaagtcgt gcaaacaaag acgaagggcc gaagaacatc
     4141 gccgatgtgc tcgacgcaac cgtggcgcgt attgagcagt tgtttcagca gccacacgat
     4201 ggcgttaccg gagtaaacac cggttatgac gatctcaaca aaaaaaccgc tggcttgcag
     4261 ccgtcggatt tgatcatcgt cgccgcgcgt ccgtcgatgg gtaaaacaac atttgcgatg
     4321 aacctcgtcg aaaacgcggc gatgttgcag gataaaccag tacttatctt ctcgctggag
     4381 atgccttcag aacagattat gatgcgttct ctggcgtcgc tgtcgcgcgt tgaccagact
     4441 aaaatccgta ccgggcagct cgatgatgaa gactgggcgc gcatttccgg caccatgggt
     4501 attttgctcg aaaaacgcaa tatctatatc gatgactcct ccggcctgac gccaacggaa
     4561 gtgcgttccc gcgcacgacg tattgcccgt gaacacggcg gcatcgggct tatcatgatc
     4621 gactacctgc aactgatgcg cgtaccggcg ctttccgata accgtacgct ggaaattgca
     4681 gaaatctctc gctcgctgaa agcactggcg aaagaactga acgtgccggt ggtggcgctg
     4741 tcccagttga accgttctct ggaacaacgt gccgacaaac gcccggtcaa ctccgacctg
     4801 cgtgaatctg gctctatcga gcaggatgcg gacttgatca tgtttatcta tcgtgatgag
     4861 gtgtatcacg aaaacagtga tttaaaaggc atcgcggaaa ttattatcgg taaacaacgt
     4921 aacggcccaa tcgggacggt acgcctgacc tttaacggtc aatggtcgcg cttcgacaac
     4981 tatgcggggc cgcagtacga cgacgaataa taattatttt atgaattagg caattaaagc
     5041 aaacacttat caaggaacac aaatgcaagc ggcaactgtt gtgattaacc gccgcgctct
     5101 gcgacacaac ctgcaacgtc ttcgtgaact ggcccctgcc agtaaaatgg ttgcggtggt
     5161 gaaagcgaac gcttatggtc acggtcttct tgagaccgcg cgaacgctcc ccgatgctga
     5221 cgcctttggc gtagcccgtc tcgaagaagc tctgcgactg cgtgcggggg gaatcaccaa
     5281 acctgtactg ttactcgaag gcttttttga tgccagagat ctgccgacga tttctgcgca
     5341 acattttcat accgccgtgc ataacgaaga acagctggct gcgctggaag aggctagcct
     5401 ggacgagccg gttaccgtct ggatgaaact cgataccggt atgcaccgtc tgggcgtaag
     5461 gccggaacag gctggggcgt tttatcatcg cctgacacag tgcaaaaacg ttcgtcagcc
     5521 ggtgaatatc gtcagccatt ttgcgcgcgc ggatgaacca aaatgtggcg caaccgagaa
     5581 acaactcgct atctttaata ccttttgcga aggcaaacct ggtcaacgtt ccattgccgc
     5641 gtcgggtggc attctgctgt ggccacaatc gcattttgac tgggtgcgcc cgggcatcat
     5701 tctttatggc gtctcgccgc tggaagatcg ctccaccggt gccgattttg gctgtcagcc
     5761 agtgatgtca ctaacctcca gcctgattgc cgtgcgtgag cataaagccg gagagcctgt
     5821 tggttatggt ggaacctggg taagcgaacg tgatacccgt cttggcgtag tcgcgatggg
     5881 ctatggcgat ggttatccgc gcgccgctcc atccggtacg ccagtgctgg tgaacggtcg
     5941 cgaagtaccg attgtcgggc gcgtggcgat ggatatgatc tgcgtagact taggtccaca
     6001 ggcgcaggac aaagccgggg atccggtcat tttatggggc gaaggtttgc ccgtagaacg
     6061 tatcgctgaa atgacaaaag tgagcgctta cgaacttatt acgcgcctga cttcaagggt
     6121 cgcgatgaaa tacgtggatt aatcgttctg taatatttga ttgtctgtgc cggatgcggc
     6181 gtgaatgcct tatccggcca ataaaatcct aaaaattcaa taagttgatg ttctttcatg
     6241 ctcttataaa ggtcgtgcct ctggcggatg ttcgtttgtc atgagtctca ctctgttgct
     6301 aattgccgtt cgctcctgaa catccactcg atcttcgcct tcttccggtt tattgtgttt
     6361 taaccacctg cccgtaaacc tggagaacca tcgcgtgttt caaaaagttg acgcctacgc
     6421 tggcgacccg attcttacgc ttatggagcg ttttaaagaa gaccctcgca gcgacaaagt
     6481 gaatttaagt atcggtctgt actacaacga agacggaatt attccacaac tgaaagccgt
     6541 ggcggaggcg gaagcgcgcc tgaatgcgca gccccatggc gcttcgcttt atttaccgat
     6601 ggaagggctt aacagctatc gccatgccat tgcgccgctg ctgtttggtg cggaccatcc
     6661 ggtactgcaa caacagcgcg tagcaaccat tcaaaccctt ggcggctcag gggcattgaa
     6721 agtgggcgcg gatttcctga aacgctactt cccggaatca ggcgtctggg tcagcgatcc
     6781 tacctgggaa aaccacgtag caatattcgc cggggctgga ttcgaagtga gtacttaccc
     6841 ctggtatgac gaagcgacta acagcgtgcg ctttaatgac ctgttggcga cgctgaaaac
     6901 attacctgcc cgcagtattg tgttgctgca tccatgttgc cacaatccaa caggtgccga
     6961 tctcactaat gatcagtggg atgcggtgat tgaaattctc aaagcccgcg agcttatccc
     7021 attcctcgat attgcctatc aaggatttag tgccggtatg gaagaggatg cctacgctat
     7081 tcgcgccatt gccagcgctg gattacccgc tctggtgagc aattcgttct cgaaaatttt
     7141 ctccctttac ggcgagcgcg tcggcggact ttctgttctg tgtgaagatg ccgaagccgc
     7201 tggccgcgta ctggggcaat tgaaagcaac agttcgccgc aactactcca gcccgccgaa
     7261 tttgggtgcg caggtggtgg ctgcagtgct gaatgacgag gcattgaaag ccagctggct
     7321 ggcggaagta gaagagatgc gtactcgcat tctggcaatg cgtcaggaat tggtgaaggt
     7381 attaagcaca gagatgccag aacgcaattt cgattatctg cttaatcagc gcggcatgtt
     7441 cagttatacc ggtttaagtg ccgctcaggt tgaccgacta cgtgaagaat ttggtgtcta
     7501 tctcatcgcc agcggtcgca tgtgtgtcgc cgggttaaat acggcaaatg tacaacgtgt
     7561 ggcaaaggcg tttgctgcgg tgatgtaatg caggaaagca ggctggagtt acccagcctg
     7621 cagtgaaatt aaactgtcgt cgctttcact atttctttgt agatgatttt tttgatgccg
     7681 ttgttctacg tgagagataa taaaacgttg tcagttcttt tattgttaag tttatcccaa
     7741 ttaactggaa ttcctt
//
LOCUS       DAJTMC010000181         7640 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.181, whole genome shotgun sequence.
ACCESSION   DAJTMC010000181 DAJTMC010000000
VERSION     DAJTMC010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7640
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.181"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            565..3978
                     /locus_tag="OO130_003603"
     CDS             565..3978
                     /locus_tag="OO130_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HCR5366862.1"
                     /translation="MEQSLNFEMLRSQWPELAELACMAERYVHSDPESCLVKLRNYTE
                     LMVRWLYRQERLPEGIKANLYDLMNADVFTSMMPEAIIMKMDALRIHGNRAAHGGRIK
                     AKDTYWLLKEAYLLGIWLYVRYAHGNVDDCPKFTLPPLTQSSGRADEKRLEDAIKAQD
                     ESRERELALQRALQQEQEKAEHLTQRLNEARARNQHVADILSIDEAETRRRLIDSRLL
                     AADWNVGEELKNTDQVTQEHPVKEQPTTSGDGYADYVLWDEAHKPLAVVEAKKTSVNA
                     EQGRIQARLYADWLEKEYGQRPVIFYTNGYDIWLWDDHKTHGYPPRRVFGFYSKESLQ
                     YLIQQRETRLPLNRVPHVKDNEGKAVAGRLYQLETIARVSERFTNKYRQSLIVQATGT
                     GKTRVAIALSKLMIDARWVKRVLFLCDRKELRKQAANAFNQFTNEPLYVVGKSKKADR
                     QNARIYIATYPGMMKIMDHFDVGYFDLIIADESHRSIYNVYGDLFKYFDALQIGLTAT
                     PIDMVSKTTFGLFGCEGRIPTANYSLEDAIADNNLVPYEVVTHTTEFLREGIKREKLS
                     DAQIRELEEQGIDPNTLEFDGKALDEAIYNKDTNRYILRNLMENGLKDRDGQLPGKTI
                     IFARNHKHALLLNELFDDMYPQFAGRFCQVIDNYDPRAEQLIDDFKGLDESTNKELTI
                     AISVDMLDTGIDVPEIVNLVFAKPVKSKVKFWQMIGRGTRLCPGLYGYDDNGKPLDKQ
                     KFRIFDHWGNFEYHELHTEEAEVTATKSLAQKRFEAWVMLGAAAQRKFDKQAVDLVAH
                     QLREQINALDEKSIAVQEKWQQKAQYSDEKVLRQLSPKTQQDLLSVLAPLMQWLDVRG
                     QSDAIRFDMDILAAQTARYTNPEELDVLWPIIVEKVERLPPHLAQVQQQGQRINQLRN
                     LSWWKQASLEELEDIRIHLRGIMHLMEKDATPKFGSIQVDITEDANLIQTETRKTNIR
                     SIDFKLYRQQVQGALEPLFQQNPVLKKIRNGEPVTQNELDELAKLVLIQNPNVDIRAL
                     KEFYPQATASLDKLLRTIIGMDSDAVEVRFAQFAADNSLTSQQLRFLSLLKNHIRDYG
                     TIEMRQLFEQPFTHIHNEGVTGVFPDIAQIARLQKIVEELGVVTDAATV"
     gene            4042..5676
                     /locus_tag="OO130_003604"
     CDS             4042..5676
                     /locus_tag="OO130_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HCR5366863.1"
                     /translation="MITGDLKSKIDGLWEDFWVGGITNPLTVIEQITYLMYSRMLDTQ
                     EQRDEKRKQIAGIDFKPRFAPEQQEFRFSHYSNLGSDEMMEVVRDGVFQHFRQLGQAD
                     ASKVTLLGNFMKDARLEIVKPSLLTKAVEVIKNLPLDRGDTKGDLYEYLLSKLTTAGI
                     NGQFRTPRHIIRTMVEMMEPNPARGETICDPACGTGGFLATSYEYLLEKYSSLESIHT
                     EIGTNERGELEEQKIFTGDLLTPWRNHVDNNMFHGYDFDTTMLRIAAMNLIMHGVDAP
                     DIHYQDTMSQSFSTNFPQASKNAFNLILANPPFTGSLDEEDIDSTLSAMVKTKKTELL
                     FLARILQMLKVGGRSATIVPQGVLFGSSKAHQSLRKTLVEDNQLETVINLPSGVFKPY
                     AGVATAILIFTKGGQTDEVWFYDLQNDGYSLDDKRNPIKDNDLPHLLASWKHYRTLRG
                     LPVDNFMGKKLASLLKQQYPEGINAGVDFKDRTQAAFVVPKADIAAQKYDLSINRYKE
                     VVYQAEEYEDPKVILKRLKDLEKEILADLDELEGML"
     gene            5685..6755
                     /locus_tag="OO130_003605"
     CDS             5685..6755
                     /locus_tag="OO130_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001438891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HCR5366864.1"
                     /translation="MVKLGDVINVHYGKALKADQRVSNGSVHVFGSSGIVGNHDKTLC
                     SYPTIIIGRKGSVGAITWAPSGGWIIDTAYYVEIKDNNKLDLRYLFYILSGIDLTKKT
                     ITTSIPGLNRDDLYDTFIKLPPFEEQKRIVDLLDKAEGIRQKREQSIKLADDFLRATF
                     ATMYGNPITNPKKWPVHLMGEIIEFKGGNQPPKSDFIFEPKQGYIRLVQIRDFKSDKY
                     ATYIPQEKAKRIFEVDDVMIARYGPPVFQILRGLSGSYNVALMKASPKENIRKGFIFY
                     LLQLPEYHDVVVKNSERTAGQTGVNLELLNKFNVPLPPIYYQDEILDRLARIEKFKEK
                     IEISLNHLEIQFLSLQKRLIGF"
     gene            complement(6880..7596)
                     /locus_tag="OO130_003606"
     CDS             complement(6880..7596)
                     /locus_tag="OO130_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5366865.1"
                     /translation="MLSETKKRIPAHLFRMIKDELYELVLTDDPKGKLYVRDSEKIEE
                     GVSTTEFVVGYGAISMVKKSESMATKGLVGLERVDLIREVVFENGYYDWGCVVNDVLP
                     NICKGNARIPIFHFLNHAKLINPDGSIINETELSGGVLSRLNITPISFQSQGWDKRRS
                     ETVPEVKVGVNELYLAYDFSFFLRMMPYMEPGLIKRDMGELLKILKKHIDEAMNTQVL
                     SSNFCKLVCIYDYIKNSHRY"
BASE COUNT         2221 a         1739 c         1792 g         1888 t
ORIGIN      
        1 gtcctgttct cccaaatatc atggtattga tacgggagat tatgccgtac aggtacgaca
       61 cgttatgtcg tgccaaaggg gagtggatag aaaaaatggc gaagatgcga aaagttgatt
      121 aatgcattga aaaaaatgaa ttaataatga acttgggtaa aagcgccatc aaaatacgac
      181 acgacctgtc gtattttgtg ttagccggga aaagatatct gctacttact aaaatgattt
      241 agtgctgaaa atatttataa gccaataaat agacaggtat tacggagtaa cgcccaggct
      301 attttaacga gcctgctgtt ttattggctc aaaaaacata tcccatagat gatcagccat
      361 acccgcgtta gtgagcaagg tatcccttga gtagccaggg ctgaagacgc ccattgatgg
      421 gcgaaaacca aggttctggc cgttaaagcg gctatgtcgt taaacacatc accaccatat
      481 gccatccggc tcacttcgtc ggccaaatta gtgtgatatt ctttccagaa tttctgttgt
      541 atccacaaca taaggcgatg attgatggag cagtctctca attttgaaat gttgcgcagc
      601 cagtggccgg aactggcaga actcgcatgt atggcagagc gctatgttca ctccgatccg
      661 gaaagctgcc tggttaagct gcgcaactac accgaattga tggtgcgctg gttgtatcgt
      721 caggagcggt tgccggaagg tattaaggct aatctttacg atttaatgaa cgctgatgtc
      781 tttaccagca tgatgccgga agccatcatc atgaaaatgg atgcgctgcg tatccatggc
      841 aaccgtgccg cgcacggcgg acgtatcaaa gctaaagata cttactggct gctcaaagaa
      901 gcgtatttgt tgggaatttg gctgtatgtt cgctacgccc acggtaatgt tgatgactgc
      961 cccaaattta cactccctcc attaacacaa tcttcaggtc gtgcagatga aaaacgtctg
     1021 gaagatgcaa tcaaggctca ggatgaaagc cgtgagcgcg aactggcgct acaacgcgca
     1081 ctacagcaag aacaggaaaa ggccgaacac ctcactcaac gcctgaatga agccagagcg
     1141 cgtaaccagc atgttgccga tattctctct attgatgaag cggaaacccg ccgtcgcctg
     1201 attgactctc gcttacttgc agccgactgg aatgtagggg aagaacttaa aaacacggat
     1261 caagtcacac aagaacatcc agtcaaagag caacccacca cctctggtga cggttatgca
     1321 gactatgtct tgtgggatga ggcacacaaa ccgttggcgg tggtggaagc aaaaaaaacc
     1381 agcgtcaatg ccgagcaagg acgaattcag gcccggctgt atgcagactg gctggaaaaa
     1441 gaatacggtc agcgtccggt catcttctac accaacggct atgatatctg gctgtgggat
     1501 gaccataaaa ctcatggtta tcccccgcgt cgggtgtttg gcttctacag caaggaaagc
     1561 ctgcaatatc ttattcagca gcgtgaaacc cgtctgccgc tgaacagagt gccacacgta
     1621 aaagataacg aaggtaaggc cgttgccgga cgtttgtacc agctcgaaac cattgcccgc
     1681 gtcagtgaac ggtttaccaa caagtaccga caatcgttaa ttgttcaggc tacgggtaca
     1741 ggcaaaaccc gcgtggcaat tgcactaagc aaactgatga ttgatgcccg ctgggtaaaa
     1801 cgcgtcctgt ttctttgcga ccgtaaagaa ctgcgtaaac aggcggcaaa tgcctttaat
     1861 caattcacca atgagccgct gtatgtggtc gggaaatcga aaaaagcaga caggcagaat
     1921 gccagaatct acatcgctac ctatcccggt atgatgaaaa tcatggacca tttcgatgtc
     1981 ggttatttcg atttgatcat cgccgatgaa tcccaccgct ctatctacaa cgtctacggc
     2041 gatctgttta agtattttga tgccctccag attggcctga cggccacgcc aattgacatg
     2101 gtgagtaaaa ccactttcgg cctgtttggt tgtgaaggac gtattcctac cgccaactac
     2161 agcctggaag atgccatcgc cgataacaat ctggttccct atgaggttgt cacacacaca
     2221 acagaattcc tgcgcgaagg tatcaaacgg gagaaattaa gcgacgcaca aatccgcgag
     2281 ctggaagaac aaggcataga tcccaatacg ctggagtttg atggcaaggc gctggatgag
     2341 gcgatctaca acaaagacac caaccgctat atcctgcgta acctgatgga aaatggcctg
     2401 aaagatcggg acggccagtt gcctggtaaa accatcattt tcgcccgtaa ccacaaacac
     2461 gcgctgctgt tgaatgaatt gttcgacgat atgtacccac agtttgccgg gcgcttctgc
     2521 caggtcatcg acaactacga tccccgcgcc gagcaactga ttgatgattt taaagggctg
     2581 gatgaaagca ccaacaaaga gctgaccatc gctatctccg ttgacatgct cgataccggt
     2641 attgatgtcc cggaaattgt gaatctggtc tttgccaaac cggtcaaatc aaaagtgaag
     2701 ttctggcaga tgattggccg tggtacgcgc ctctgtccgg ggttgtacgg ttacgacgat
     2761 aacggcaaac cgctggataa acagaaattc cgcattttcg atcactgggg caattttgag
     2821 tatcacgagc tgcataccga agaggcggaa gtcacagcga caaaatcgct tgcgcaaaaa
     2881 cgcttcgaag cgtgggttat gctgggggcc gctgcgcagc gtaaattcga caaacaggcg
     2941 gtggatttag ttgctcacca gcttcgcgag caaatcaacg cactggatga aaagtcgatt
     3001 gccgtacagg aaaaatggca gcagaaagcg cagtacagtg atgaaaaggt gctgcgccag
     3061 ctttctccga aaacacagca ggatctgcta tctgttctgg ccccgttgat gcagtggctg
     3121 gacgtgcgcg ggcaaagcga tgccatacgc tttgatatgg atattctggc ggcgcaaact
     3181 gcccgttata ccaacccgga agagctggat gttctctggc cgatcatcgt cgaaaaagtg
     3241 gagcgcctgc cgccgcattt ggcgcaggtg cagcaacagg gacaacgaat caatcagctt
     3301 cgtaatttaa gctggtggaa gcaggccagc ctggaagagc tggaggatat tcgcattcat
     3361 ttgcgcggta tcatgcacct gatggaaaaa gacgcgacgc ctaaatttgg ttcgatacag
     3421 gtggatatta ccgaagatgc gaacctgatc cagacggaga cccgcaaaac taatatccgc
     3481 tcgattgatt tcaaactcta tcgccagcag gttcaggggg cgctggagcc gctgttccag
     3541 caaaacccgg ttctgaagaa aatccgcaac ggcgagccag tcacgcaaaa cgagctggat
     3601 gagctggcga agctggtgct gatccagaac cccaacgttg atattcgtgc gttgaaagag
     3661 ttttatccgc aggcaaccgc cagcctggat aaacttttgc gtaccatcat cgggatggac
     3721 agcgacgcgg tggaagtgcg ttttgcccag ttcgccgctg ataacagcct gaccagccaa
     3781 caactgcgct tcctgtcatt gctgaaaaac cacattcgcg attacggcac cattgaaatg
     3841 cggcagctct ttgaacagcc ttttacgcat atccacaacg aaggcgttac cggtgtgttc
     3901 cctgatatag cgcaaatcgc ccgcctgcaa aagatagtcg aagagctggg tgttgtgacc
     3961 gacgcagcga cggtataata cacgcatgac aagccccggc agcggggctt ttgtatttaa
     4021 aacaggatat aaacgctaac aatgattact ggcgacttaa aaagtaaaat cgacggacta
     4081 tgggaagatt tctgggtggg tggtatcacc aacccgctga ccgtaatcga acagatcacc
     4141 tatctgatgt attcccggat gctggatacc caggaacaac gcgacgaaaa gcgtaaacaa
     4201 atcgcgggta ttgattttaa accgcgtttt gcgccagaac agcaggagtt ccgtttcagt
     4261 cactatagca accttggctc ggatgagatg atggaagtgg tgcgcgatgg cgtattccag
     4321 catttccgtc agctcggcca ggccgatgct tcgaaggtga cgctgctggg caactttatg
     4381 aaagatgctc gtctggagat tgttaagccg tcattgctga ccaaagcggt tgaggtaatc
     4441 aaaaacctgc cattggatcg cggcgacacc aaaggcgacc tttacgaata cctgttaagc
     4501 aagctgacaa ctgccggaat caacggacag ttccgcacac cgcgccacat tatccgcacg
     4561 atggttgaaa tgatggagcc gaacccggcc cgcggcgaga cgatttgcga tcccgcctgt
     4621 ggcaccggtg gttttctggc aaccagctat gaatatctgc tggagaagta cagctcgctg
     4681 gaatccattc atactgagat tggcaccaac gaacgtggcg agctggaaga gcaaaaaatc
     4741 tttaccggcg atctgctgac accgtggcgt aaccatgtgg ataacaacat gttccacggt
     4801 tacgactttg acaccacaat gctacgtatc gccgccatga acctgattat gcacggcgtg
     4861 gatgcgcctg atatccacta tcaggacaca atgagccaga gtttcagcac aaacttcccg
     4921 caggccagta aaaacgcctt caacctgatt ctggcgaacc cgccgtttac cggttcactg
     4981 gacgaggaag atatcgactc cacgctgtcg gcaatggtga aaaccaaaaa aaccgaacta
     5041 ctgttcctgg cgcgtattct gcaaatgctg aaagtgggcg ggcgcagtgc cactatcgtg
     5101 ccgcagggcg tgctgtttgg ctctagcaag gcgcaccagt cacttcgcaa aacgctggtg
     5161 gaagataacc aactggaaac ggtgatcaat ctgccttctg gtgtatttaa accttacgct
     5221 ggcgtggcga cggcgatcct gatctttacc aaaggcggtc aaacggatga ggtctggttc
     5281 tacgatctac aaaatgacgg ctacagcctg gatgataagc gcaacccgat aaaagacaac
     5341 gatctgccgc atctgctggc aagctggaag cattaccgta ctttacgcgg gctaccggtt
     5401 gataacttta tgggtaagaa gttagcctcg ttgcttaaac agcagtaccc ggaagggatt
     5461 aatgctggcg ttgattttaa agatcgcacg caggcggcgt ttgttgtacc gaaagcggat
     5521 attgctgcgc agaaatacga tctatccatc aaccgttata aagaagtcgt gtatcaggcg
     5581 gaggaatatg aagatccgaa ggtgatattg aagcggttaa aggatctgga aaaagagatt
     5641 ctggcggatt tggatgagct ggaggggatg ctgtgagttc ggcgatggtg aagttagggg
     5701 atgttattaa tgttcactac ggaaaagcat taaaagctga tcagcgtgta tcgaatggaa
     5761 gtgtccatgt gtttggtagt tcaggaattg tcggcaacca cgataaaacg ctttgcagct
     5821 atcccacgat aattattggc cgcaaaggca gcgttggtgc tattacctgg gctccaagcg
     5881 gaggctggat cattgatact gcttattacg ttgagatcaa agataataat aagttagatc
     5941 tccgatactt gttttatatt ctgagcggaa ttgatctcac taagaaaacg attaccacaa
     6001 gtattcctgg attaaataga gacgatctct atgatacatt tattaaatta ccaccttttg
     6061 aagagcagaa acgaattgtg gatcttctcg ataaagctga gggtatacgc caaaaacgtg
     6121 agcaatccat caaactggct gatgattttt tgcgtgctac gtttgctaca atgtatggga
     6181 atcctattac aaaccccaaa aaatggcctg tccatcttat gggagaaatt attgaattta
     6241 aaggtggaaa tcaaccacct aaaagtgatt ttatttttga acctaaacag ggttatatta
     6301 gattagtaca aattagagat tttaaatccg ataaatatgc gacgtacatt ccgcaagaaa
     6361 aagcaaaacg tattttcgaa gttgatgatg ttatgattgc tcgttatggg ccacctgtgt
     6421 ttcaaatact tagaggttta tccggcagct acaatgtagc tttaatgaaa gcttcaccca
     6481 aagaaaatat taggaagggg tttatattct atcttctaca acttccggaa tatcatgatg
     6541 ttgttgtaaa aaactctgaa agaacagcag gacaaaccgg tgtaaatctt gaactattaa
     6601 acaaattcaa tgttcccctc ccacctatat actatcaaga tgagatatta gatagattag
     6661 ctagaattga aaaatttaaa gaaaaaatag aaatatcatt aaatcattta gaaatacaat
     6721 ttttatcact acagaagaga ttaataggtt tttaaatcct tagtatcgaa ataaacatta
     6781 atttattata ttaaattaag atattttagg aaattaaggc gtcactctta ggtaacgcct
     6841 ttttttacta tctcgccccc taagaaggtt atatcttact taatacctgt gactattttt
     6901 gatataatca taaatacaca ctaatttgca aaaattactt gacaatacct gagtattcat
     6961 agcttcatct atatgttttt tcaatatttt gagaagctct cccatatcgc gcttgattaa
     7021 tccaggctcc atataaggca tcattcttaa aaagaaactg aaatcataag caagatataa
     7081 ctcattgacc cccaccttga cctcaggaac ggtttcagag cgtcgtttat cccatccttg
     7141 agactgaaaa ctaataggag tgatatttaa tcgtgataaa acgccacctg agagttctgt
     7201 ttcattaatt atactcccat caggattaat taatttagcg tgatttaaga agtgaaaaat
     7261 aggaatgcgc gcattgcctt tgcatatatt tggcaaaaca tcattaacaa cacatcccca
     7321 atcataatat ccattttcaa atacaacctc tcggatgaga tctacacgct ccagtccaac
     7381 caatccttta gttgccatcg attccgattt ttttaccatc gaaatagcac catatcccac
     7441 aacaaactca gttgtactga ccccttcttc aattttctct gaatccctca catataattt
     7501 tcctttcgga tcatcagtta agaccaactc ataaagttca tcttttatca ttcggaataa
     7561 atgtgctgga atcctttttt tagtttcact cagcacctca aatatttctt tataggatgg
     7621 tgcctgaaca tattttaccg
//
LOCUS       DAJTMC010000182         7627 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.182, whole genome shotgun sequence.
ACCESSION   DAJTMC010000182 DAJTMC010000000
VERSION     DAJTMC010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7627
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.182"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(<1..314)
                     /locus_tag="OO130_003607"
     CDS             complement(<1..314)
                     /locus_tag="OO130_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HCR5366866.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRRLKEDYPDK
                     VL"
     gene            complement(308..1546)
                     /gene="preT"
                     /locus_tag="OO130_003608"
     CDS             complement(308..1546)
                     /gene="preT"
                     /locus_tag="OO130_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HCR5366867.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAQPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEVGNTLTLEQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLQRARQAQGDISIPQSALIIGGGDVTMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFTSARELGVSIIDGFTPVAVEGNKVTFKHVRLSGELT
                     MAADKIILAVGQHARLDAFAELEPQRNTIKTQNYQTRDPQVFAAGDIVEGDKTMVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            complement(1943..2182)
                     /locus_tag="OO130_003609"
     CDS             complement(1943..2182)
                     /locus_tag="OO130_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HCR5366868.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(2185..2904)
                     /gene="sanA"
                     /locus_tag="OO130_003610"
     CDS             complement(2185..2904)
                     /gene="sanA"
                     /locus_tag="OO130_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HCR5366869.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(3054..3938)
                     /gene="cdd"
                     /locus_tag="OO130_003611"
     CDS             complement(3054..3938)
                     /gene="cdd"
                     /locus_tag="OO130_003611"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HCR5366870.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPYAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(4068..4763)
                     /locus_tag="OO130_003612"
     CDS             complement(4068..4763)
                     /locus_tag="OO130_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HCR5366871.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYGSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(4760..5158)
                     /locus_tag="OO130_003613"
     CDS             complement(4760..5158)
                     /locus_tag="OO130_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HCR5366872.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            5397..6347
                     /gene="dusC"
                     /locus_tag="OO130_003614"
     CDS             5397..6347
                     /gene="dusC"
                     /locus_tag="OO130_003614"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HCR5366873.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIDKLR"
     gene            complement(6734..6817)
                     /gene="yohP"
                     /locus_tag="OO130_003615"
     CDS             complement(6734..6817)
                     /gene="yohP"
                     /locus_tag="OO130_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohP"
                     /protein_id="HCR5366874.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            7041..>7627
                     /locus_tag="OO130_003616"
     CDS             7041..>7627
                     /locus_tag="OO130_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HCR5366875.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAVATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEW"
BASE COUNT         1968 a         1960 c         1909 g         1790 t
ORIGIN      
        1 atcaatactt tgtccgggta atcttctttc agccgacgca gggcggccag attctcctcc
       61 agcggatgct cggcaatctg ctccatattt ttgaagccga taaaaccggt atcttctttc
      121 accagatgat caaaacgcgg cgagacttcg ttggcgataa aaaagccgat cgttttaaac
      181 accacaccgc cccaacctgt gtcgtaggct ttggcacaca tctcatagca gttgcctacc
      241 ggcgaagaag agaggcagaa cgggttggga aacttcacgc cgcaaaaagt aatcgaaaga
      301 tctttcgtta acatgagcaa gctccctcta aatagtgatg aatcgcctcg gcggcttctt
      361 tcccggtttt cacggcatag accatggttt tgtcaccctc aacaatatcg ccagcagcaa
      421 agacttgcgg gtcgcgggtc tggtaatttt gtgttttgat ggtgttacgc tgcggctcta
      481 actccgcaaa ggcatctagt ctggcatgct gaccgacggc gagaataatt ttatctgccg
      541 ccatcgtcag ttcgcccgat aaccgtacat gcttaaacgt gactttattg ccttccacgg
      601 ctactggcgt gaatccatcg atgatcgaaa cgcccagctc ccgggcgctg gtaaactctt
      661 tttcgcttgc cggaaactca tctaactctt cacgcgctac gcaagttacc gcctgacagc
      721 cgagaacttt cagcgtgctg gctacgtcca tcgtgacatc accgccgccg ataattaatg
      781 cgctttgtgg aatgctgata tcgccttgcg cctgtcgtgc acgttgcaag aagtcgacgg
      841 caatctcaac gtcactatgc tcaaacagcg gtagcccgga accgcttgat aaccccacgg
      901 tgaccagtac cgcgcggttt tctgctttca gctgctcaag ggtgagtgtg ttaccgactt
      961 cgttgttgca cttaatggtc acgcccattt tttcaatacg ggcgatctct gcatccagta
     1021 ctgattgcgg taaccggaat tgcggaatgc cgttacgcaa ccagccaccg ggttgtgctt
     1081 ctttctcata gatcgtgacg tcataaccct ggtttgtcag tgtcacactg gcctgcaatc
     1141 cggcaggacc tgcgccaata atcgcgactt tgccgagcgt tttagtaccg ggctgataaa
     1201 tttccatccc ggtttgttgt tcaaaatcag taacaaaacg ctgtaagcgg ccgatatcaa
     1261 tgggagcatc aacaccggca cgggtacaac cgctttggca taatttctcc gtcgggcaca
     1321 ctctggcaca aacggcaccg agggcattat tttcgcgaat tgtctcggca gcgcctttaa
     1381 aattacgaaa gtagattgag cgaataaatt tccccggatc ggtctgtgcc gggcaagcct
     1441 gactacaggg agcgtcgtga cataataaac agcgagaggc ttctttaatc gccagtaacg
     1501 gcgtaaaagc cggagtgagt tcatccagat aattttgttg cggcatgtga tcttccttaa
     1561 tgctatgagt aattaaggcg aacgctaaat atactcgtca tacttcaagt tgcatgtgct
     1621 gcgtctgcgg gttattcggc ccatccatgg gcctcacccc ttatggggcc gctgcaggca
     1681 gcgttcaaat ccgctccatg cggatttgtc gctcacccca gtcacttact tatgtaagct
     1741 cctggggact cactcacttg tcgccttcct gcaactcgaa ttatttagag tataaatatt
     1801 gcgtgctgat attaaggcta tgcgcgtggg gaaatttata ctgtgaaagg attcacaaat
     1861 agctgcggtt cagaatgtta tatccgcagc ttattgagtg aagtaataat tattctcaat
     1921 aattaacaga aaagagaaaa tattaacgat agaaaataag ataaacaatc atgccgatag
     1981 ataaaattcc gaagccgata tatgccacca tataagcgaa aaagagtccg ggattttttg
     2041 ctcgccagac atacaccggt tccggtgcat ttttaacccg tttatcaatc gcgcctgcgc
     2101 gccagccttt ccatgcttcg cggaaacaaa atatcggaat aaggaaaaaa acggcaacga
     2161 caaaaaactg ctgtacatcc ataactactt tccttgtttc ttttgtaatt caagtaactg
     2221 ttcgggtgtg acggcgggat acccctgcgc atcttccggc acctggtgca tagccggaat
     2281 agggaccagc ggccctaaaa aacgcggttc acgtttaaaa atataaaggt cagccagcgc
     2341 accgaaacgg gcggcaaatt cgcgaatacg tactgacagc atatctttcg gtgacggtac
     2401 ggcataacac tgagcctgaa tccccatatg cagcgcaata aataatgccc gctcacagtg
     2461 gaaacgttgg gtgataataa tgaaatcatt agtatcgaaa actttgcgtg tacgcacgat
     2521 ggaatccagc gtacgaaagc ctgcgtaatc gagaacaata tctgatgggt cgacaccagc
     2581 agcgattaaa tctttgcgca tggtcatcgg ctcattataa ctttgcaatg cgttatcgcc
     2641 gctcagtaat agataattta ccttaccgct gttataggca ttaatcgctc cttgaatgcg
     2701 gtagcgataa tactgattaa ttacgccagt acgataatat tttgctgttc cgagcaccac
     2761 accgacctgg cggtagggga gatcctgcaa ttcgtcgtag atataaggcg cggttttcca
     2821 gctcatccag cgatcgaggc cgagcacagt caacaacagc aagccgatca ggactaacag
     2881 gctgaggaac acgcgcttta acatgcagga gactcgatct aaggaaaatt tccatcaggc
     2941 tactttaccc gctggttttg cgcaagaaac ggcaaactgt ttgccgggaa tttcgacacc
     3001 aggagagggc tggcaagccg gacaaggcgt tcacgccgca tccggcacca ggcttaagcg
     3061 agaagcactc ggtcgatact gtgacagccg agagctttca gcgttgcgga ggtggcatcc
     3121 cactgaatca acggcgcatc ggctttttct gccagaaccg cgcgctggat atccgggtaa
     3181 tcataaccct tgagattcaa cagaattaac gctccctgca acggtggcag agtcgggttg
     3241 aatgcggcgt tttcagcgta gctgccactg aaaatacgac cgtctttaca ttccagcgcg
     3301 acaccgcttg gcgacttact gtaaggcatg tgcgaacggt ttgccgctgc aatcgccgcc
     3361 tgagaaagcg catcaccagt cagcgcatag ccgtgatcct gctcgtccat cagcagcgtt
     3421 ttaatctcca gatctttcgg cccaaaggca tacggcagat agtcacgcag cgcgtgtgcc
     3481 tcgcggcccg gcagatgaat acgcagatcc agaccgctgt tcagttcatt cataaactga
     3541 cggcagtgac cacaaggcgt gtagttaacg gtgatggctg caagcgcttt ttcaccactc
     3601 aaccaggcgt ggctgatcgc gctttgttcg gcatgaacgg tttgctgcat tgtcgcacca
     3661 ataaattcca tattggcacc gaaataccag gttccgctca caccgcgcgc aattgcgcca
     3721 acattaaaat tcgacaatgg cgtacgcgca caggccgctg ccagcggaag tagtgcgaat
     3781 gccagcgcgt cttcgtccag ccccgttgcg ctcttcagcg atgagacttg ctccccggtc
     3841 aacaaagcgg ggaagtactt gtctgccaga ataggttcca gtgcagattg caagttatcc
     3901 gcaagttggg caaaagcggt ttgaaaacgt ggatgcatgg cgttgcctca taatcatgta
     3961 atgggataac agtttacgaa cccgttatgg ctttatatgt gatctgaatc tcattaatta
     4021 tgcaacgcgt gaaacttaca tcaaaaatgc gcgacgcatc gcaaatttta gcccattact
     4081 gccagaataa tcgggaaaag gaacggtgcg atcagcgaag taattatccc gcataacacc
     4141 agcgctagcg aactaaatgc accttcctga taatccagct cggcgcagcg cgccgtaccg
     4201 agggcgtgcg aggcagtccc cattgccaga ccgcgcgcag ctttggtacg aatacgcatc
     4261 gcattaagca atgtatggcc aaataccgcg ccgaggatgc cgacgaaaat cacgcaaact
     4321 gcgctgattg ccggaatacc gccaatactg ccgccaaccg ccattgcaat aggcgtggtg
     4381 actgatttcg gcaggattga cgcggcgatt tccggtgaag cgcccatcaa caatgccacg
     4441 gaagtccccg tcaccatcgc aaccacgctg ccgataaaac agatggtgat gatcgatttc
     4501 cagcgcgcgc ggatctggtg tagctgctca tataaaggat aggccagcgc gacgaccgcc
     4561 ggttgcagca gatcgtttag cacttcgcta cctttaaagt agctgccgta agagatgccg
     4621 gtcagcatta aaaaagggat gatcaccacc attgctacca gcaacgggtt aagcaaggga
     4681 aacttatacc gtgccgccag tttgcgggcg gcaaaaaaga cgatcaatgt cagtggtaat
     4741 gaccaccaga tattagccat cattcttctg atcctttctg acctacgact ttacgttcac
     4801 cgtgcaccag ttgcgaactc cagctcacca ccagaaaaac caccagtgta ctgacagcac
     4861 aggaaaccac taccgggcca aactgtgcgc ggagcaaatc aaaatattgc atgacgccta
     4921 cgccaatcgg cacaaacaat agcgccatat agcgaatcag tacgtagcac cccggattga
     4981 cccattttgc cggaagaatt tgcaaggcca gcaggacaaa caggatcagc atcccgatga
     5041 tgctgcccgg aatggttacc ggtagcaggg aagcaatgaa aatgcctgca tacaggcagg
     5101 cataaatcag gacgaaagcg cgtaaatatt gccagataat gttcagtgtc ttactcataa
     5161 tcaacacccc agatgagatg aattcatcat acaattaatt cgtaaaacgt gctaccgatc
     5221 acattaagaa atcagcgctt attttttcgc tccccggcat tacgcaatgc gcaacgaact
     5281 caggcataca tcaagcatag agagggatta cagtttgcag caatccctct ttgcagacct
     5341 ttaatccgtt gaccataggc gctaacatag cgcctcattt ttttgcgggt gaagatatgc
     5401 gtgtgttact ggctccgatg gagggtgtgc ttgactctct ggtgcgtgaa ttgctgaccg
     5461 aagttaacga ctacgatctg tgcatcaccg agtttgtccg cgtggtggat caactgctgc
     5521 cggtaaaagt ttttcatcgc atttgccctg agctacaaaa cgccagccgg acaccatccg
     5581 gtacgctggt gcgcgtgcag ttgttgggtc agttcccaca atggctggca gagaatgccg
     5641 cccgtgcggt ggaattaggt tcctggggcg tggatctcaa ttgcggctgc ccgtcgaaaa
     5701 cggtcaacgg tagcggtggt ggggcgacgt tactcaaaga tcctgaactc atctaccagg
     5761 gtgcaaaagc gatgcgtgaa gctgtaccgg cgcatttgcc cgtcagcgtg aaagtgcgtc
     5821 tgggctggga cagcggtgag aagaaatttg aaatcgccga tgcggttcaa caggctggtg
     5881 ctacggagct ggtggtgcat ggacggacaa aagagcaggg ttaccgcgcg gagcatattg
     5941 actggcaggc gattggcgag attcgccagc ggctgaatat tccggtgatt gccaacggtg
     6001 aaatctggga ctggcagagt gcgcaacaat gtatggcgat cagcggctgc gacgcagtga
     6061 tgattggtcg cggggcgctc aatattccca acctgagccg ggtggtaaaa tataacgaac
     6121 cgcgaatgcc gtggccggag gtggttgctt tgctgcaaaa atatacccgt ctggaaaagc
     6181 agggcgatac cgggttatat cacgttgcgc ggattaaaca gtggttgagt tatttgcgta
     6241 aagaatacga tgaagcaacg gaattatttc agcatgttcg ggtgttgaat aattcccctg
     6301 atattgcaag ggctattcag gcaattgata tcgataagct gcgataatag tggaatcgca
     6361 gtttatcaga gataatgaat cgcttattca gcttcatcat cttgttgcgc tcctttcatg
     6421 agctaagcca catattgcca ctggcgcaag gagcgcgcag ggggaggaca atcgcctcct
     6481 ccccctgccc cctgggctct ggcgaacaaa atcgccgctg cgcggtacct tcagcttatc
     6541 cccttcggct accgggtcgg gcgcgaggta acgtccttgt aagacgcgcc ctcagcccac
     6601 atccatgtgg gctgccccgg ccttcgggga acgcttcagc gattttgacg ccaccaacac
     6661 cggagctgtt tgcaaatcaa gtacacaaaa tatctagttc ctgacctgac aacttatatg
     6721 ccatatctga tttttaaaat atcatcttaa atacgccagt caccaccagt agcccaatca
     6781 ggaaaataat caatacagcc cagagtataa ttttcattgg cgttccttta tttgtgagtg
     6841 ttgaataagt ataggaaaaa acgaagccct ttgccgttat ccataaacga ttaagataaa
     6901 tcggaagata tcattcactt agtctatatt tttctgggta tgtcgtttta ttgctactga
     6961 tgtattgcca cgcgaaacta aaaaatatat cgcaacacac ggtaatctcc gcgcaccgtt
     7021 tcttacctga ttattccccc atgaatcgtg attcctttta tccagccatc gcctgttttc
     7081 cgctgttatt gatgctggcc gggtgtgcgc ctatgcatga aacccgccag gcgttaagcc
     7141 agcaaacgcc cgctgcacaa gttgacaccg cattacccac ggcgctgaaa aatggttggc
     7201 cagacagcca atggtggctg gagtatcacg ataatcagct cacttcatta attaacaatg
     7261 cactacagaa cgcaccggat atgcaggtgg ctgaacagcg cattcagcta gctgaagccc
     7321 aggcgaaggc agttgccact caggatggtc cgcagatcga cttttcggcg gatatggagc
     7381 ggcaaaaaat gtcggcagaa ggcttaatgg ggccgtttgc tctgaacgat ccggccgcag
     7441 gtacgaccgg cccgtggtac accaacggta cttttggctt aacggcgggc tggcatctcg
     7501 atatctgggg aaagaatcgg gcggaggtta ctgcccgcct gggtacggtt aaagcacggg
     7561 cggcggaacg cgagcaaacc cgccaattgc tggctggcag cgtagcccgc ctgtactggg
     7621 agtggca
//
LOCUS       DAJTMC010000183         7472 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.183, whole genome shotgun sequence.
ACCESSION   DAJTMC010000183 DAJTMC010000000
VERSION     DAJTMC010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7472
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.183"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            <1..339
                     /locus_tag="OO130_003617"
     CDS             <1..339
                     /locus_tag="OO130_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCR5366876.1"
                     /translation="QTEAFLNAYGLSRFAPLGYDPRDLPIRDLAGYRKKGNHDGDPII
                     FYTFPAAFEQEIAKGFNTKQFAEVLKNAGMLTPPTSGRGYQGRVREDGRQIRVYVLNF
                     MAEESSQPEE"
     gene            714..1148
                     /locus_tag="OO130_003618"
     CDS             714..1148
                     /locus_tag="OO130_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCR5366877.1"
                     /translation="MTAQISAYGRLVADPQTRTTTNGNNMAMARLAVSLPCNAAEAGE
                     STFWLGVIAFGKQADALAKHQKGDLVSVAGNMQLNQWTGQDGGTQQGYQVVADSVISA
                     RTVRPGGKAGQRGQATDALRRAQQPSRDEYDQRPPFDDETPF"
     gene            1159..1800
                     /locus_tag="OO130_003619"
     CDS             1159..1800
                     /locus_tag="OO130_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002436679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5366878.1"
                     /translation="MSTQPIIIEFGSYRWPRHLAIHEADHAIAAWFFGIQEVEIALSG
                     NNRVAKTYLSGDVDECGAVAVWHANYPRNVAEVKSYTLSNDDTRLRLIDQVCRAMVIS
                     LAGIVAQSRYTGDDINALMETSGKADMKNVRDLSEVYRVAGGEDADIQRISLSRAEAL
                     IALKWWDVIALAKILENRNHMPASNFQRIMGDIDKPVPKPMTLAELDELVGEE"
     gene            1797..2147
                     /locus_tag="OO130_003620"
     CDS             1797..2147
                     /locus_tag="OO130_003620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5366879.1"
                     /translation="MKPYILAAGLISSAPALSAIHCGGFTIQPDRYRTTINGDVVHIV
                     DAQFYGAPRDFSQAAIKLLPDAITTTDRQYRIETQGGNALLELMTRENPPRVLNRERC
                     DAGLTSFGWYTSAP"
     gene            2841..3452
                     /locus_tag="OO130_003621"
     CDS             2841..3452
                     /locus_tag="OO130_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5366880.1"
                     /translation="MRDRTRSYTTDLPRIGLPFLANMRSRLADAEPGTQLYTQTESGT
                     LYAFRQADSYAMTINGVTRAIRTTTTKAGYGVREWYACPHCMKRAAKLYIGKKDIGCR
                     ACWKLHYKSQSADILDRMRMKIRQQRCAIWGSNDLANNLFNDIRMFPKPKGMRWATFD
                     RKRAELSVMEMAYWQAFSPVVDRITGVIERKAGKAARGIGLTL"
     gene            3990..4532
                     /locus_tag="OO130_003622"
     CDS             3990..4532
                     /locus_tag="OO130_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid pRiA4b ORF-3 family protein"
                     /protein_id="HCR5366881.1"
                     /translation="MYQLKITIRDSKPPIWRRVLVPEQIPFSELHAVIQLAFGWNDEH
                     LYMFEKGRKGDPGSEYRVWGEDESMGNAAITPLWAALQNDGDKLVYTYDFGDWWDCVI
                     VLEKQTHDTSNQPISCLRGKGTTPVENSGGLHGYNELLLQARESDNPEQAEIHNFLML
                     DIERRVYDLSSINDRLQTLN"
     gene            complement(4525..4884)
                     /locus_tag="OO130_003623"
     CDS             complement(4525..4884)
                     /locus_tag="OO130_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5366882.1"
                     /translation="MRMTSRKKEILSYFEPDNLEWVTGEIGAPPFDVSGVAYLLHGMV
                     SFDKRHQLESTRRTLESMVAGGLLERVTVYESRQIRRGGETNATVVRYGLPGQCAVMR
                     DTGGADNAISGEYMRVS"
     gene            5318..6142
                     /gene="dinD"
                     /locus_tag="OO130_003624"
     CDS             5318..6142
                     /gene="dinD"
                     /locus_tag="OO130_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HCR5366883.1"
                     /translation="MNEHHQPFEEIRHYGTEGQEFWSARELAPLLDYRDWRNFQKVLA
                     RATQACEASNQAASDHFVETTKMVVLGSGAQRELEDVHLSRYACYLVVQNGDPAKPVI
                     AAGQTYFAIQTRRQELADDEAFRQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAI
                     DFAIFQNHGYRGLYGGLDQKAIHQRKGLKKNQNILDHMGSTELAANLFRATQTEEKLK
                     RDGVNSKQQANTTHFDVGRKVRQTIQELGGTMPEELPTPQVSIKQLENSVKITEKK"
     gene            6434..7051
                     /locus_tag="OO130_003625"
     CDS             6434..7051
                     /locus_tag="OO130_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HCR5366884.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(7048..7472)
                     /gene="ligB"
                     /locus_tag="OO130_003626"
                     /gene_synonym="yicF"
                     /pseudo
     CDS             complement(7048..7472)
                     /gene="ligB"
                     /locus_tag="OO130_003626"
                     /gene_synonym="yicF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312549.2"
                     /note="this ligase is similar to LigA but it lacks the
                     C-terminal BRCT domain; catalyzes strand joining of nicked
                     DNA in the presence of a divalent cation and NAD+;
                     internal stop; incomplete; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
BASE COUNT         1999 a         1835 c         1907 g         1731 t
ORIGIN      
        1 cagacggagg cgtttttgaa tgcctatggg ctgagtcgat tcgcgccgct gggttatgac
       61 ccgcgcgatt tacccatccg tgatttagcc gggtacagga agaaggggaa ccacgacggc
      121 gacccgataa ttttctacac cttcccggca gcgtttgagc aggagattgc aaagggcttc
      181 aataccaaac agtttgccga agttctaaag aatgcaggaa tgcttacacc accaacgagc
      241 gggcgaggct atcaggggcg agtaagggag gatgggcgac agattcgcgt ctatgtactc
      301 aatttcatgg cagaggaaag cagccagcca gaagaataat tatttcttca catgcgtagt
      361 tttagtgttg tctcggttgt ctttgttgtc tcaatttgta attaagattg atttataagg
      421 attttatttt aatttctgag acaacaccga gacaacattt agccattttg agacaacagg
      481 gcgaaagtgt gtttttagtc tggcaggcag tgagacaaca tttatgccgg gtgagacaac
      541 acgaaattgc aaatgttgtc tcagcaaaac ccagacgtgg cgcggcctgt agagccgtga
      601 gacaacaaag acaacagaga caacactatt tttgcttact atgaaaaaaa atcggcaggt
      661 gtcccacatt aagaaatcgt cattagcaaa actaataatc cagtgagggg aaaatgacag
      721 cacaaatttc agcatacggg cggctggtgg cagatcccca gacccgcacc acaacgaacg
      781 gtaacaacat ggctatggcg cggctggcgg tgtctctccc ctgtaatgcg gcagaggcgg
      841 gagagtcaac cttctggctg ggcgttattg cttttgggaa gcaggccgat gcgctggcaa
      901 agcaccagaa aggcgacctt gtgagcgtgg caggcaatat gcaactcaac cagtggacag
      961 ggcaggacgg cggtacacag caggggtatc aggtagtagc cgacagtgtg atcagcgcca
     1021 gaacggtacg ccccggcggg aaggcaggac agcggggaca ggcaacggac gcgctgagac
     1081 gtgcgcagca gccatcccgt gatgaatacg accaacgccc accattcgac gatgaaacgc
     1141 cattctgaga gggaagatat gagtacgcaa cccatcatta ttgagttcgg cagctatcgc
     1201 tggcccagac atcttgcaat acatgaagca gatcatgcga tagccgcatg gtttttcggc
     1261 atacaggagg tagaaattgc gttatcgggt aataaccgag tggcaaaaac ctaccttagc
     1321 ggcgatgttg atgaatgtgg agctgtcgcg gtatggcacg ccaactatcc acggaatgtg
     1381 gctgaggtta agtcttacac cctctcgaat gatgacacca ggttacgtct gatagaccag
     1441 gtatgccggg ctatggttat ctctctggct ggtatcgtcg cgcaatcacg ctatacgggg
     1501 gatgatatta acgcactgat ggaaacgtcc gggaaagcag acatgaaaaa tgttcgtgat
     1561 ttatcagagg tataccgggt ggctggtggg gaggatgctg atatacagag aatatccctt
     1621 tcccgtgctg aggcgctgat tgccctgaaa tggtgggatg ttatcgcgct ggcgaaaata
     1681 ctggagaacc gtaaccatat gcctgccagc aacttccagc gcattatggg tgatattgac
     1741 aagcctgtac caaagcctat gacgctggct gaactggatg aattggtggg tgaagaatga
     1801 agccatacat tctggcagcc ggcctgatct cctcggcacc ggcactatct gctatccatt
     1861 gtgggggctt taccatacag ccagaccgtt atcggacaac gattaacggc gatgtggttc
     1921 atattgttga tgcgcaattt tacggcgcac cgcgagactt tagccaggcc gccataaagt
     1981 tattgccgga tgccattacc actaccgata ggcaataccg catagagacg cagggaggca
     2041 atgccttgct tgagttgatg accagagaaa acccgccacg tgtgcttaat cgtgagcgct
     2101 gcgatgccgg gttaacaagt ttcggttggt acacttctgc gccgtgatgt tcgttccgag
     2161 cgcagcctaa aatattggac tgttcttgac ggatttaaaa ctctaatgga gccaagtcct
     2221 tgccagtagc gggctaggag cctaattgtg attcaggtcg tcaaaaatat tcaggtttgc
     2281 ggcattggtt cgcagtacgc agtccggtac gcggcaaaac tgcgcattta acgtgcgtac
     2341 cacctgacga attctaacgc cggcattgca aacagatgcg tgcgcaaaat ggctaagacg
     2401 tctgatattg ctggctttgg ctcaatcagt ttggaggtgg cggtatcagt acgatgctaa
     2461 tttttgctaa ggttttagta cgcaactcag gtacgcactc tcaccagata cgaaaccgat
     2521 tcgcagttgc gtaccagcct gtaaggcgca ttatcgctgg gtttctacag tcgaatgcag
     2581 tacgcaaagt ggaaaatcct ccgaatacga aacccgagca ttgcgcagta aattgcgaat
     2641 tgcgcagtta acaaaatatc tccgcaaaac gaaacggata tcaatgcgca aaccatcctc
     2701 aacccccgcg cggttactgg ctttattgat gtgaaattgc aaaattggtg cgcaatttac
     2761 agtaaaatac gcatattatt tcgtattcaa tttgcgcacc gttcgcagtt aatgcgcagt
     2821 aattcgcagg aggccagcca gtgagagaca gaacgcgcag ctataccacc gacctgcccc
     2881 gtatagggtt gccattcctt gcgaacatgc gtagcaggct ggcggatgct gaaccgggta
     2941 cgcagcttta tacccagacc gaaagcggaa cgttgtatgc cttccggcaa gccgacagct
     3001 acgccatgac aatcaacggc gtgacgcggg ctattcgcac cacaaccacg aaggccgggt
     3061 atggcgtccg ggaatggtac gcttgccccc actgtatgaa gcgggctgca aagctgtata
     3121 tcggcaagaa agacattggc tgccgggcgt gctggaaact tcactataaa agccagagtg
     3181 ccgacatact ggacaggatg cgcatgaaga tacgccagca gcgatgcgcg atatggggca
     3241 gcaatgacct tgccaataat ctgtttaatg acattcgcat gttcccaaaa ccaaagggga
     3301 tgcgctgggc tacgtttgac aggaagcggg cagagttatc agttatggag atggcttact
     3361 ggcaggcatt cagcccggta gtggacagaa tcacgggcgt tattgagcgt aaagcaggaa
     3421 aagcagccag aggcattggc ctgactctat aaaagcaaaa cgcccgcacg gggcgtcaga
     3481 gaacgggata gacttctgcg acaatttgca ttcgccgcca atatagcaag ccgtccacat
     3541 caagcaataa actatcaccg ggagggggag gccaaatctc tgcaatcctg agccttaagg
     3601 actgcccgcc ccatcgtttt ttcatacccg cgaaaaatga aatttaatcc gggcgtgttt
     3661 catccttcca gagggtatga cgtatgacac cacgcgaaat agccttattg accatcgcca
     3721 aacttgagca tggaggccac cagcttacac aggcagatca acgggagata gaacgctcag
     3781 ttaatgccga tatagcccgg cgcgacaggt tccgcgaaat gatgcgagca cctgcctacc
     3841 agtggaagaa gccagcgccg cgcaggtaga tacgggatta ttccgcatgt aacttatgcg
     3901 tattaagcaa atttccttta ttcaccttta cagaattgtg agtacgtcag tatcgttatc
     3961 cttccgattc gcatttattc aggatttgca tgtaccagct aaagataacc atccgagaca
     4021 gcaaaccacc tatatggcgg cgtgtgctgg ttccagagca aatccccttt agtgaacttc
     4081 acgcggtgat ccagttggct tttggctgga atgacgaaca cctgtatatg ttcgagaaag
     4141 ggcgtaaagg tgatcccggt agcgagtatc gcgtatgggg tgaggatgaa agcatgggta
     4201 atgcggcaat cacaccacta tgggcagcgc tccagaatga tggtgacaag ctggtttaca
     4261 cgtatgactt tggcgactgg tgggattgcg tcattgtgct ggagaagcaa acacacgata
     4321 cgagcaatca gcccattagc tgcctgcgtg ggaaaggcac caccccggtg gaaaactcag
     4381 gcggcttgca tggttacaat gaattgctat tacaggccag agagtctgat aatcctgagc
     4441 aggccgagat ccacaatttc ctgatgctgg atattgagcg ccgggtttac gacctgagca
     4501 gcatcaacga cagattgcag actcttaact gacccgcata tattcgcctg atattgcgtt
     4561 atcagcgccg ccagtatcgc gcataacagc gcactgaccg ggcaaaccgt agcgcacgac
     4621 cgttgcgtta gtctcaccgc cgcgccgtat ctggcgcgat tcatacactg tcacccgctc
     4681 aagcagtcca ccagcaacca tgctttccag tgtgcgccgg gttgattcga gctggtgacg
     4741 cttatcgaac gacaccatgc catgaagcag gtaggccaca cccgacacat cgaacggcgg
     4801 cgcaccaatc tcaccagtca cccattcgag gttatccggt tcaaagtagc taagtatctc
     4861 ttttttgcgg ctggtcattc tcatagctgg caggttcctt attgtgggat agcactatca
     4921 tacaataagg cgcaaaagat aagaggccag aacagcaagc atgagagcat aaaacgagca
     4981 gccaaagaca gcgcctcaaa ataccgtgta cacaagggtg tacctactga gaaaacgcat
     5041 tacaagaaac acagcaacca cgggcgttta aagccactac tcaataaaca gattcaactc
     5101 gtaacgccaa ttcttacttt tcagccttca caaatgccgc ccactcaaac agagcggcat
     5161 ttttcttccc cgcaacttca attctgtttt gcgtgcctgc tccagatttt gcgatgtttt
     5221 tttgcccagc acactgagaa cgtgagatac tcacaactgt atataaatac agttacagat
     5281 ttactttctt tgcaatcgat atcacatgga gtgggcaatg aacgaacatc atcaaccttt
     5341 tgaagaaata cggcattatg gcactgaagg gcaagaattt tggtcagcta gagaattggc
     5401 accgttactg gattatcggg actggcgcaa ttttcaaaag gtactggccc gcgcgactca
     5461 agcgtgcgaa gccagcaatc aagctgcatc tgaccatttc gttgaaacca ccaaaatggt
     5521 tgtattaggt tcaggggctc aacgagaact tgaagatgtt catctctccc gctatgcctg
     5581 ttacctggta gtacaaaacg gcgaccctgc gaaaccggtt attgcggcag ggcaaactta
     5641 ttttgctatc cagacccgac ggcaggagct ggctgacgat gaagctttca ggcaacttcg
     5701 tgaagacgaa aagcgtcttt ttctgagaaa tgaattgaaa gaacataaca aacaattggt
     5761 tgaggcagcg cagcaagctg gggtagcgac agctatcgat ttcgccatat ttcagaatca
     5821 tggttaccgg gggctgtatg gtggattaga tcagaaagct attcatcagc ggaaggggct
     5881 gaaaaagaat cagaatatcc tggatcatat gggttcaaca gaactggcgg ctaatctctt
     5941 tcgagctacc caaacagaag aaaaactcaa gcgggatggc gtgaattcaa aacagcaagc
     6001 aaacaccaca cactttgacg tgggtcgcaa ggtgaggcaa accattcagg aacttggcgg
     6061 aaccatgcct gaggagttgc cgaccccgca ggtcagcatc aagcagttgg aaaacagtgt
     6121 aaaaattaca gagaagaaat agcagaaact aaaaactctt tttcttaatt gagatacccg
     6181 atgcgtaagc caaagtccag gtgcagtgaa cacaatggct aaacattgca cctttctttt
     6241 ccacccctca attttaacct attttttctt atgcattttc tcagacaaga actcagaata
     6301 atgcatctac gctacagaaa atagcgattt cacataacta caagttatca atttcctctt
     6361 accttaaaaa atctcaatcg tgacaatgcg cacaaatcgc taccctgcca gacagatttt
     6421 tagggagaga accatgctgt tacacatttt gtatttggtt ggcatcactg ccgaagccat
     6481 gacgggggcg ctggcggccg gacgacggcg catggataca tttggcgtaa ttattattgc
     6541 gaccgccacc gcaattggcg gagggtcagt gcgcgatatt ctgctgggcc actatccgct
     6601 cggctgggtc aaacacccgg aatatgtgat tatcgtcgct accgccgcag tactcaccac
     6661 tatcgttgct cctgtaatgc cttacctgcg caaagtgttt ctggtgctcg atgcgctcgg
     6721 actggtggtc ttttctatca tcggcgcaca ggttgcactg gatatggggc acggcccaat
     6781 tattgccgtt gtcgcggcgg taaccaccgg cgtgtttggc ggcgttttgc gcgatatgtt
     6841 ctgtaaacgc atcccactgg tattccagaa agagttgtat gctggtgtct catttgcctc
     6901 cgccgtgctg tacatcgcgc tgcaacacta tgtttctaac catgatgtgg tgattatctc
     6961 caccctggta ttcggctttt ttgcccgctt actggcgcta cgtcttaagc tgggattacc
     7021 ggtcttttac tacagccacg aaggtcacta aggttcaaaa cctgtgatct gctgggcagc
     7081 cagccaactg cccagcttct tgatttgcgc attttccttc cattcaataa cctgtctggc
     7141 gcgcccagat ccagtccccg gcagctgctg tcagaactgc tccgtgctaa ataaaagttg
     7201 cgactaggac cgttcatcac tggcattaag cgccgcccgg gttagcggta ttcccattgc
     7261 catcacccag cgagtaaaag gctgcttacg agccagatta aactgatgcc ataactgcgc
     7321 acttttactt ttcgcgatcc ccggcgtgtt ctgtaattgc tctggcgtta ataaaagcca
     7381 agaaaagata tgctcaaagc gatgagtctg atgcagcgcg cgccaaccgg cctcaccaat
     7441 gccatccagc ccaagaacct gttttgaccc ca
//
LOCUS       DAJTMC010000184         7448 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.184, whole genome shotgun sequence.
ACCESSION   DAJTMC010000184 DAJTMC010000000
VERSION     DAJTMC010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7448
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.184"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            1..1459
                     /locus_tag="OO130_003627"
                     /pseudo
     CDS             1..1459
                     /locus_tag="OO130_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
     gene            1470..2543
                     /locus_tag="OO130_003628"
     CDS             1470..2543
                     /locus_tag="OO130_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5366885.1"
                     /translation="MNKYIKQWCFAVFMLSLSSVALAAPKGICTPDNGVFHSTLDFSG
                     YLITANENKVGTTFNTTVTNGSSYPGRCHCDTGNVGEFPYIYYTSKINQALTYAGVHS
                     NINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKYNCNRIEPLSISSGAKA
                     IVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMADVYIRGDITAPQSCEIN
                     NLQPVYFDFKEIPAADFSSVVGSAVITHKITKTVTIECENLGILNTDDISTSFYATEP
                     NTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMGKSTVYGEKSGSVTFSAAPAS
                     LTGARPAPGQFTATATITVEIVR"
     gene            2791..3831
                     /gene="pstS"
                     /locus_tag="OO130_003629"
     CDS             2791..3831
                     /gene="pstS"
                     /locus_tag="OO130_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HCR5366886.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            3918..4877
                     /gene="pstC"
                     /locus_tag="OO130_003630"
     CDS             3918..4877
                     /gene="pstC"
                     /locus_tag="OO130_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HCR5366887.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            4877..5767
                     /gene="pstA"
                     /locus_tag="OO130_003631"
     CDS             4877..5767
                     /gene="pstA"
                     /locus_tag="OO130_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HCR5366888.1"
                     /translation="MAMVEIQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            5858..6631
                     /gene="pstB"
                     /locus_tag="OO130_003632"
     CDS             5858..6631
                     /gene="pstB"
                     /locus_tag="OO130_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HCR5366889.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            6646..7371
                     /gene="phoU"
                     /locus_tag="OO130_003633"
     CDS             6646..7371
                     /gene="phoU"
                     /locus_tag="OO130_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HCR5366890.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
BASE COUNT         1871 a         1724 c         1944 g         1909 t
ORIGIN      
        1 cagatgttga tgtcagcgtg ggtgaattgc gcgatgaaga cggatttaca ccggacgtcc
       61 ttcaggcgca aatacttcat ggtttttccc acgggatcac tttatatgga ggtatgcagg
      121 ctgctgaaaa ttatggttct gcagctctgg gtgtcggtaa agatcttggc gctttgggcg
      181 caatttcttt cgatgtgaca catgctcgtg cgaattttag ccatgatgat acagaaacgg
      241 gtcagtcata tcgctttctc tattcaaaac gatttgacga cacagacact agcttgcgcc
      301 tggttggcta tcgttactcc accgagggct actataccct caatgagtgg gcatcgcggc
      361 gcaacagccc tgaagacttt tgggaaacag gtaaccgacg tagtcgcgtg gagggaacgc
      421 taacgcagtc gttggggaga gattatggca atttatacct gacattaagc cggcaacaat
      481 actggcatac cgatgatgtc gaacgattaa tgcaatttgg ctacagcagt agctggaagc
      541 gtctctcgtg gaacgtctcc tggagttatt ccaatactgc cagacagggg acggggaaca
      601 accatgccag tgataacacc agtgagcaga tctacatgct ctctttatct gttcctttat
      661 cgggctggtg gggtaatagt tacgccacct attctgtttc gcaaaacgat aattccggta
      721 gctcacatca agcggtacgg cgctggaaag aaataacctt tcatggaatt taatgcagtc
      781 ctataacagt catgatgatg aggttggcgg taatatgtcc ctgacctatg atggctctta
      841 tggcacggtg aacggcagct ataactacag ccaaaattcc cagaggctga attatggtat
      901 cagaggggga attctggcac acagcgaagg ggtaacgtta agtcaggagt taggtgaaac
      961 tattgctctt gttaaagcac ctggggccgc cgggttagaa atagataata tgcgcggtgc
     1021 tgcgacggac tggcgcggct atacggtcaa gacacagcta aacccttatg atgaaaatcg
     1081 ggtagcaatc agcgataact atttctcgaa gtcgaatata gaacttgata ataccgtcgt
     1141 tacgatggtt cccacgcgtg gtgcagtggt taaagcggag tttgtgactc atgtgggtta
     1201 tcgcgttctc ttcagagtgt taaatgcaaa tggtaaaccg gtaccttttg gagccattgc
     1261 tgcgatacaa gatgcaagtt tggcagattc aggaattgtc ggtgaccgtg gcgaacttta
     1321 tctttctggt ctaccagaaa aaggacaggt tacgttatcc tggggagaaa acgcctcaac
     1381 aaaatgcatc ttcaattatt cactttcgac accagaaagt gagagcggat taattgaaca
     1441 gggtgtgaca tgtcattaaa aggattttta tgaacaagta tatcaaacag tggtgctttg
     1501 ctgtgtttat gctctcgtta agtagtgtag cacttgcagc tcctaaaggt atctgtaccc
     1561 ctgataatgg cgtatttcac agtacgcttg atttctcggg atatcttatt accgcgaacg
     1621 aaaataaggt tggaacgaca tttaacacca ctgtaactaa tggatcctca tatcctggac
     1681 gctgtcattg tgatactggt aacgtaggag aattccccta tatctattat acgtcaaaaa
     1741 taaaccaggc attaacttat gctggggttc actctaatat taattattat gatcttaatc
     1801 cgaaccttga tgttgggatt gctatagata ttcttggggt tgggtatgtg aatgcacctt
     1861 ttgaatatca tgctaacaac ccgtctggta atacaaagta caattgcaac cgcattgaac
     1921 ctttaagtat atctagtggg gctaaagcga tagtatattt ttatatcaag aagacatttg
     1981 caggaaaatt gattattcct gaaacaaaga tagtgacatt gtatggaaca attagccgtg
     2041 acaccccggt ggattactca cagccgatgg ctgatgttta tattcggggc gatattacag
     2101 ctccgcaaag ttgcgaaata aataatttac agccagttta ttttgatttt aaagaaatac
     2161 cagctgcaga tttttcatct gttgttggaa gtgcggtaat aacacataaa attactaaaa
     2221 cggtcactat tgagtgtgaa aacctgggga tactgaatac cgatgatatc agtacgtctt
     2281 tttatgctac cgaacccaat acggacaatt caatggtcgt aacttcaaac tctaacgtgg
     2341 ggataaaaat ttacgacaaa aataataagg aaatcaaagt gaacggtggt gagttgccaa
     2401 cagacatggg taaatcaaca gtgtatggtg aaaaatcagg tagtgtaact ttttcagctg
     2461 ctcctgcgag ccttactgga gctcgcccag ccccggggca atttactgca acagcgacga
     2521 taactgttga gattgtgcgc taataggtct gacaacgatt gttcatcaca tcgttgtcat
     2581 ctcacccatt tctttatcac ttttaaatgc atcatgacaa aaagatgaca tgtgggattt
     2641 tcatcttcac atttaaaatc agtaagttat ttggtaattc ctttctgtca taaaaatgtc
     2701 atattcctta catataactg tcacctgttt gtcctatttt gcttctcgta gccaacaaac
     2761 aatgctttat gaatcctccc aggagacatt atgaaagtta tgcgtaccac cgtcgcaact
     2821 gttgtcgccg cgaccttatc gatgagcgct ttctctgtgt ttgcagaagc aagcctgaca
     2881 ggtgcaggtg caaccttccc tgcgccggtg tatgccaaat gggctgacac ttaccagaaa
     2941 gaaaccggta ataaagttaa ctaccagggt atcggttctt ccggtggcgt aaaacagatt
     3001 atcgctaata ccgttgattt tggtgcctct gacgcgccgc tgtctgacga aaaactggct
     3061 caggaaggtc tgttccagtt cccgaccgtg attggcggcg tggtgctggc ggttaacatt
     3121 ccagggctga agtctggcga actggtgctg gatggtaaaa ccctcggcga catctacctg
     3181 ggcaaaatca agaagtggga tgatgaagcc atcgccaaac tgaatccggg tctgaaactg
     3241 ccttcacaaa acattgctgt agtacgccgc gcagatggct ccgggacttc cttcgtcttc
     3301 accagctacc tggcgaaagt gaacgaagag tggaaaaaca acgttggtac tggctctacc
     3361 gtaaaatggc cgatcggtct gggcggtaaa ggtaacgacg gtatcgccgc gttcgttcag
     3421 cgtctgccgg gtgcaattgg ttatgttgaa tatgcttacg cgaagcagaa caacctggcg
     3481 tacaccaaac tgatctccgc tgatggtaaa ccggttagtc cgaccgaaga aaacttcgct
     3541 aatgcagcaa aaggtgcaga ctggagcaaa accttcgctc aggatctgac caaccagaaa
     3601 ggcgaagatg catggcctat tacctctacc acgttcattc tgatccacaa agatcagaag
     3661 aaaccagaac aaggcacaga agtgctaaaa ttcttcgact gggcgtacaa aaccggggct
     3721 aaacaggcga acgacctgga ttacgccagc ctgccggata gtgtagttga acaggttcgc
     3781 gctgcgtgga agaccaatat taaagacagt agcggtaagc cgctgtacta ataaaactcc
     3841 aggccgggta cggtgtttta cgccgcatcc ggcattacaa aatgactttg taaacgcgtt
     3901 taactgaaga gtaacttatg gctgcaacca agcctgcttt taacccaccg ggtaaaaagg
     3961 gcgacataat tttcagcgtg ctggtaaaac tggcggcgct gattgtgcta ttgatgttgg
     4021 gtggcattat tgtctctctg atcatctcct cctggccgag cattcagaaa tttggtctgg
     4081 ctttcctatg gaccaaagag tgggatgcac cgaacgatat ctacggggcg ctggtgccga
     4141 tctacggtac gttggtgact tcgtttatcg cgctgctgat cgccgtcccg gtgagtttcg
     4201 gtatcgccct gttcctgact gagcttgcgc ctggctggct gaaacgcccg ctgggtatcg
     4261 ccattgagct gctggcagcc attccaagta tcgtttacgg catgtggggc ctgtttatct
     4321 ttgcgccgct gttcgccgtt tactttcagg agccggtcgg caatatcatg tcgaatatcc
     4381 cgattgttgg cgcgctgttc tctggccccg catttggtat cggtatcctc gcggcaggcg
     4441 tgatcctcgc catcatgatt attccgtaca ttgcggcggt aatgcgtgat gtgttcgaac
     4501 aaaccccggt gatgatgaaa gagtcggcct acggtattgg ctgcaccacc tgggaagtta
     4561 tctggcgtat cgttcttccg ttcaccaaaa atggtgttat cggcggcatc atgctggggc
     4621 tgggccgcgc gctcggtgaa accatggcgg tgacctttat catcggtaac acctaccagc
     4681 tcgacagcgc ctcgctgtac atgccgggca atagtatcac ctctgcgttg gcgaacgaat
     4741 ttgcggaagc ggaatccggt ctgcacgttg ccgcactgat ggaactgggc ctgatcctgt
     4801 ttgtgattac cttcatcgtc ctcgccgcat cgaagtttat gattatgcgc ctggctaaga
     4861 atgagggggc acgctaatgg ctatggttga aatccaaacc actgcggcgc tggctgaatc
     4921 tcgccgcaaa atgcaggcgc gtcgccgcct caaaaaccgt attgcgctga cgctctcgat
     4981 ggcgacgatg gccttcggcc tgttctggct gatctggatt ttaatgtcca ccatcactcg
     5041 cggtatcgac ggtatgtcgc tggcgctgtt cactgaaatg acgccgccgc ccaatacgga
     5101 aggtggtggt ctggcgaacg ccctggcggg tagcgggctg ttaattttgt gggccacggt
     5161 attcggtacg ccgctgggca ttatggcggg gatttatctg gcggaatatg gtcgtaaatc
     5221 ctggctggca gaagtgattc gcttcattaa cgacattctg ctctccgcgc cgtcgattgt
     5281 ggttggtctg tttgtttaca ccatcgtggt ggcgcagatg gagcacttct ctggctgggc
     5341 gggcgtgatt gccctggcgc tgttgcaggt gccgattgtt atccgcacca ccgagaacat
     5401 gctgaaactg gtgccggaca gcctgcgtga agcggcttat gcgctgggta caccgaagtg
     5461 gaagatgatc tctgcgatta cgctgaaagc gtcggtgtcc gggatcatga ccggtatcct
     5521 gctggcgatt gcccgtattg ctggtgaaac cgcgccgctg ctgtttaccg cgctctccaa
     5581 ccagttctgg agcacggaca tgatgcagcc gatcgccaac ctgccggtga cgatctttaa
     5641 gtttgcgatg agcccgtttg cggaatggca acaactggcc tgggccgggg tattgattat
     5701 taccctgtgc gtactgctgc tgaacattct agcgcgcgtt gtttttgcga agaataaaca
     5761 cggttaacac tttgccggat gcggcgtgaa cgccttatcc ggcctacggt aagcctgatt
     5821 agcgaagtgc atcaggcacg atgaggaaaa gattgcaatg agtatggttg aaactgcccc
     5881 gagtaaaatt caggttcgta atttgaactt ctactacggc aaattccatg ccctgaaaaa
     5941 catcaacctg gatatcgcta aaaaccaggt tacggcgttt atcgggccgt ccggctgcgg
     6001 taaatcgacg ctgctgcgta ccttcaacaa aatgtttgaa ctgtacccgg agcagcgtgc
     6061 ggaaggtgaa attctgcttg atggcgacaa catcctgacc aactctcagg atatcgcact
     6121 gctgcgtgcg aaagtgggca tggtgttcca gaaaccgacg ccgtttccga tgtccatcta
     6181 cgacaacatc gcttttggcg ttcgtctgtt tgagaagctc tcccgtgccg acatggacga
     6241 gcgcgtgcag tgggcattga ccaaagccgc attgtggaac gaaaccaaag ataaattgca
     6301 ccagagcggt tactctctct ctggtggtca gcaacagcgt ctgtgtattg cgcgtggtat
     6361 cgccattcgc ccggaagtgc tgctgctcga cgaaccgtgt tcggcgctcg accctatctc
     6421 taccgggcgt attgaagagc tgatcaccga actgaagcag gattacaccg tggtgatcgt
     6481 cacccacaac atgcagcagg ctgcgcgttg ttccgaccac acggcgttta tgtacctggg
     6541 cgaattgatt gagttcagca acacggacga tctgttcacc aagccagcga agaaacaaac
     6601 agaagactac atcaccggtc gttacggttg attcaggagt gcgttatgga cagtctcaat
     6661 cttaataaac atatttccgg ccagttcaac gccgaactgg aaagtatccg cacgcaggtg
     6721 atgaccatgg gcggcatggt ggagcagcag ctttctgatg caatcaccgc gatgcataac
     6781 caggacagcg atctggcgaa gcgcgtgatc gaaggcgaca agaacgtcaa catgatggaa
     6841 gtggcgatcg atgaagcctg cgtgcgcatt atcgccaaac gtcagccgac ggcgagcgac
     6901 ctgcgactgg ttatggtgat cagtaaaacc attgccgagc tggagcgtat tggcgacgtg
     6961 gcggacaaaa tctgccgtac tgcgctggag aaattctccc agcagcatca gccgttgctg
     7021 gtaagtctgg agtcgctggg ccgtcatacc atccagatgc tgcacgacgt gctggacgcg
     7081 ttcgcgcgga tggacattga cgaagcggta cgtatttatc gtgaagataa aaaagtcgat
     7141 caggaatacg aaggtattgt tcgtcaactg atgacctaca tgatggaaga ttcgcgtacc
     7201 attccgagcg tacttactgc gctgttctgc gcgcgttcta tcgaacgtat tggcgaccgc
     7261 tgccagaata tttgtgagtt tatcttctac tacgtgaagg ggcaggattt ccgtcatgta
     7321 ggcggtgatg agctggataa actgctggcg ggaaaagata gcgacaaata attcaccaga
     7381 caaatcccaa taacttaatt attgggattt gtggtaatgc tgccaactta ctgatttagt
     7441 gtatgatg
//
LOCUS       DAJTMC010000185         7435 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.185, whole genome shotgun sequence.
ACCESSION   DAJTMC010000185 DAJTMC010000000
VERSION     DAJTMC010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7435
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.185"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            167..274
                     /locus_tag="OO130_003634"
     CDS             167..274
                     /locus_tag="OO130_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5366891.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(327..1085)
                     /locus_tag="OO130_003635"
     CDS             complement(327..1085)
                     /locus_tag="OO130_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HCR5366892.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVTYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            1372..2301
                     /gene="pfkB"
                     /locus_tag="OO130_003636"
     CDS             1372..2301
                     /gene="pfkB"
                     /locus_tag="OO130_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416237.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HCR5366893.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISASQKQG
                     IRCIVDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            2402..2692
                     /gene="ghoS"
                     /locus_tag="OO130_003637"
     CDS             2402..2692
                     /gene="ghoS"
                     /locus_tag="OO130_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HCR5366894.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            2798..3658
                     /locus_tag="OO130_003638"
     CDS             2798..3658
                     /locus_tag="OO130_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HCR5366895.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(3699..4235)
                     /locus_tag="OO130_003639"
     CDS             complement(3699..4235)
                     /locus_tag="OO130_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HCR5366896.1"
                     /translation="MTYQQAGHIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            4382..5050
                     /gene="hxpB"
                     /locus_tag="OO130_003640"
     CDS             4382..5050
                     /gene="hxpB"
                     /locus_tag="OO130_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HCR5366897.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            5213..5791
                     /locus_tag="OO130_003641"
     CDS             5213..5791
                     /locus_tag="OO130_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR5366898.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQIINTLQIQFHRLIKH"
     gene            5936..7327
                     /gene="tcyP"
                     /locus_tag="OO130_003642"
     CDS             5936..7327
                     /gene="tcyP"
                     /locus_tag="OO130_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HCR5366899.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLAA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGILLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHR"
BASE COUNT         1868 a         1809 c         1836 g         1922 t
ORIGIN      
        1 attttcgata tatatgttga tgcataatat tattttttca taaaacgcag aaacagtgac
       61 caggtgtaca acgccaccca acggctgtaa taacaaaaaa tcccttgatg cccgtccctt
      121 ttgttacact ccgttatcac gcacaagaga tatgcaggac actggaatgc cgactaaacg
      181 ctttgataaa aaacactgga agatggtggt agtgctactg gcaatctgtg gcgctatgtt
      241 gttgctacgt tgggcagcaa tgatttgggg ctgagcagtg acaaacgaag acagccagac
      301 cgcataacac ggtccggcag ataattttac atgctgtagc ccagcgataa ggttgtccga
      361 cggtcggtat gttccggtgc agactctgga ggttcagagt tccatgtaac gttgtaagtc
      421 accttcagtc caaaatgttc attaatagca acatttaatg cgctttcgga gttcagcgtt
      481 gtgtcttccg cgccaaagac cgaaacaccc tgcgtaaatt tagcgttgtc agtcaactgc
      541 catgcataag ccccggaggc atagcccagc ggctgagttt cactggcatt gtcggtatat
      601 ttgtcgtaac gcacacctgg accgaattca aagcggaaac tgtgtaccgg gccattcaaa
      661 aactgacgac cataacccgc ggtcaacaca tcgcgctctc gatagccgtt ataacggtct
      721 gtcagccagc ttgcctgacc aaataaatag tcataatcag ttaaattaaa gcggctacgc
      781 ccgcccgccg catatttttc tgaagaacgc tcatcattag aagaagtatt actggcgttc
      841 ccccacagcg accaggcagt ggtttgtcca taccaggtca tggtggtgtc agccgtaagt
      901 gaggagcttt tcgtattgcc tgattgtgca agatatcctg cgttcagatt accttcgaaa
      961 ggttttttag cgctggcagg atcgtccatg acagtaaaaa cggaatcatc ggcagctgca
     1021 ttcagtgacg caaacatgcc ccccgccaac ataacgatgg caggaactgt cttcaaaagc
     1081 ttcatttatc aagagtccgt acaacaaaaa aagagaccat cgcggtcccg gaaactttct
     1141 taaggatcaa agattagcgt ccctggaaag gtaacgaatt ataaaaaggc gcgaataact
     1201 tagcaatgta ttcttatttc attttttgaa taagcatgtg gcgaaaacag atttttattt
     1261 atatatattt atctgcaaaa ttttaaataa agctccaata aatcatattg ttaatttctt
     1321 cactttccgc tgattcggtg ccagactgaa atcagcctat aggaggaaat gatggtacgt
     1381 atctatacgt tgacacttgc gccctctctc gatagcgcaa caattacccc gcaaatttat
     1441 cccgaaggaa aactgcgctg taccgcaccg gtgttcgaac ccgggggcgg cggcatcaac
     1501 gtcgcccgcg ccattgccca tcttggaggc agtgccacag cgatcttccc ggcgggtggc
     1561 gcgaccggcg aacacctggt ttcactgttg gcggatgaaa atgtccccgt cgctactgta
     1621 gaagccaaag actggacccg gcagaattta catgtacatg tggaagcaag cggtgagcag
     1681 tatcgttttg ttatgccagg cgcggcatta aatgaagatg agtttcgcca gcttgaagag
     1741 caagttctgg aaattgaatc cggggccatc ctagtcataa gcggaagcct gccgccaggt
     1801 gtgaagctgg aaaaattaac ccaactgatt tccgcttcgc aaaaacaagg gatccgctgc
     1861 atcgtcgaca gttctggcga agcgttaagt gcagcactgg caattggtaa catcgagttg
     1921 gttaagccta accaaaaaga actcagtgcg ctggtgaatc gcgaactcac ccagccggac
     1981 gatgtccgca aagccgcgca ggaaatcgtt aatagcggca aggccaaacg ggttgtcgtt
     2041 tcccttggtc cacaaggagc gctgggtgtt gatagtgaaa actgcattca ggtggtgcca
     2101 ccaccggtga aaagccagag taccgttggc gctggtgaca gcatggtcgg cgcgatgaca
     2161 ctgaaactgg cagaaaatgc ctctcttgaa gagatggtcc gttttggcgt agctgcgggg
     2221 agtgcagcca cactcaatca gggaacacgt ctgtgctccc atgacgatac gcaaaaaatt
     2281 tacgcttacc tttcccgcta acaaaaacat tcccccagca ttgggggaat caccaccaac
     2341 ctgtcggcaa cgcgtttctc cgactatgct caaaagtcat gtgataacaa aggggtgaac
     2401 tatggccagt ggcgatcttg tccgttatgt cataaccgtc atgttgcatg aggatacatt
     2461 gactgaaatt aacgagttga ataattacct gactcgcgac ggttttttgc tcaccatgac
     2521 ggatgatgag ggaaatatcc atgagctggg gactaacact tttggactta tcagtaccca
     2581 aagtgaagaa gaaattagag aactggtttc ggggcttacc caaagtgcaa ccggcaaaga
     2641 tcctgaaatc accatcacga cctgggagga atggaatagc aacagaaaat aaatggtttt
     2701 tgggcaataa tcagtctgtg gtgtgcgtta gctcgtgttt ttacaccgca ttcttgcgct
     2761 aaccttatga tctggcagac aacatgggag agacatcatg tggcaggcaa tcagtcgtct
     2821 tttgagcgag cagttaggtg aaggcgaaat cgaactgcgt aatgaactgc ctggcggaga
     2881 agtccatgcc gcatggcatt tgcgctatgc aggacatgac tttttcgtca aatgtgatga
     2941 aagggaactg cttcccggtt ttaccgccga agccgaccaa ctggagttac tgtcgcgtag
     3001 taaaaccgtc accgtgccta aggtttgggc agtaggcgct gaccgtgact acagttttct
     3061 ggtgatggat tatctcccac ctcgtccgct ggatgcgcat agcgcattta ttcttggtca
     3121 gcaaattgcg cgtttacatc aatggagtga ccaaccacaa tttggcctcg attttgataa
     3181 ctcgctctcc acaactccac agcccaacac ctggcaacgt cgctggtcaa cgttttttgc
     3241 tgaacaacgg attggctggc agttggaact ggcagcagag aaagggatcg ctttcggcaa
     3301 tatcgacgcc atcgtcgagc atattcagca gcgtctggcc tcgcatcaac cgcagccttc
     3361 tctgttgcac ggcgatttat ggtccggcaa ctgtgcgctg ggtccggatg gcccgtacat
     3421 tttcgaccct gcctgctact ggggtgaccg agagtgcgac ctggcgatgt taccgctgca
     3481 tactgaacag ccgccacaaa tctatgacgg ctatcagtca gtatccccgc tacctgccga
     3541 tttccttgaa cgtcaaccgg tttaccaact ctacacgctg ttaaatcgtg caaggttatt
     3601 tggcggtcag catttggtta ttgctcagca gtcactggat agattattag cagcatgata
     3661 tgggttgaag ataatggccg ctctgtgcgg ccttttgatt aaataaaccc aagcagagaa
     3721 aagaacacat aacctgcagc aatacaaata accggcagga tatacagtga gaaaaactgc
     3781 aggaaaatag tatggtgcgg aacaacaata cgggactcaa tttgttcacg cgttagcccc
     3841 tcttcccctt tggctttttc cagaatgagt tgatcttcaa caccttcccg caagaagcgc
     3901 gcctggcgac tcattcgggc accggaatcc tgcaacgcca gtccgacaaa aatcaggatg
     3961 aaaatcaccc agaacataac gttcacgcca ccattaaaat tgggcgtcgg ggagttatac
     4021 cagaacagat tcaaaaaagg cgtattcgcc tgcatcatat cgatcatgac atgagtaaag
     4081 tcgagcatga ccgcattaat gccttcctgg ttttcctgcc gggtattcat aaatttcagc
     4141 aaagaaatca atgtagagat caatgcaggt ataaaaatca cccaacccaa aatccttttc
     4201 aaaacagcaa tgtgtccagc ttgttgatac gtcatgagtt ctccttgatt aagacgcgtc
     4261 gtttcactta gtttacctgt agatatctgt tttcgcccat tctttaaagg cgatatgata
     4321 ggcgcttaat cataagcacg gcttaatacc ttacacataa tgctctaaag gagaggttgt
     4381 aatgtcaacc ccgcgtcaga ttcttgctgc aatttttgat atggatggat tacttatcga
     4441 ctcagaacct ttatgggatc gagccgaact ggatgtgatg gcaagcctgg gggtggatat
     4501 ctcccgtcgt aacgagctgc cggacacctt aggtttacgc atcgatatgg tggtcgatct
     4561 ttggtacgcc cggcaaccgt ggaatgggcc aagccgtcag gaagtagtag aacgggttat
     4621 tgcccgtgcc atttcactgg ttgaagagac acgtccatta ttaccaggcg tgcgcgaagc
     4681 tgttgcgtta tgcaaagaac aaggtttatt ggtgggactg gcctccgcgt caccactaca
     4741 tatgctggaa aaagtgttga ccatgtttga cttacgcgac agtttcgatg ccctcgcctc
     4801 ggctgaaaaa ctgccttaca gcaagccgca tccgcaagta tatctcgact gcgcagcaaa
     4861 actgggcgtt gacccactga cctgcgtggc gctggaagac tcggtaaatg gcatgatcgc
     4921 ttctaaagca gcccgcatgc gttccatcgt cgttcctgcg ccagaagcgc aaaatgatcc
     4981 acgttttgta ttagcaaacg tcaaactctc atcgctgaca gaactcaccg caaaagacct
     5041 tctcggttaa tgaccagggg cagtgatcac gctgcccctg gttctttatc tgaatttgca
     5101 ttcaactgac ggattaatcg tcaatttaag agaaagagtt acaccgtcac cacttccgtg
     5161 cactgtataa aaatcctata ctgtacgtat cgacagttta gtgagtttta tcatgacggc
     5221 ggaaggtcac cttctctttt ctattgcttg tgcggtattt gccaaaaatg ccgagctgac
     5281 gcccgtgctg gcacagggtg actggtggca tattgtccct tccgcaatcc tgacgtgttt
     5341 gttaccggac atcgatcacc caaagtcgtt tcttgggcag cgattaaaat ggatatcaaa
     5401 accgatcgcc cgcgcttttg ggcatcgtgg ttttacccac agtctgctgg cggtatttgc
     5461 gctgctggca accttttacc ttaaggttcc ggaaggctgg ttcattccgg ctgatgcgct
     5521 acaaggaatg gtgctgggtt atttgagcca catacttgcc gatatgctga cacccgccgg
     5581 tgttcccctg ctctggccat gccgctggcg tttccgcttg cctatcctag ttccccaaaa
     5641 gggcaaccaa ctggaacgtt ttatctgtat ggcattattt gtctggtcgg tatggatgcc
     5701 ccattcatta cccgagaaca gcgctgttcg ttggtcatcg caaataatca ataccttgca
     5761 gatccagttt catcggctta ttaagcatta ggttgaatac taaaaagaca aaaatcacct
     5821 ttctggaata agcaattcca tttgaatata agagccagct cacagttctg ttaatcttgc
     5881 gccaacacta tgactgctac gcagtgatag aaataataag atcaggagaa cggggatgaa
     5941 ctttccatta attgcgaaca tcgtggtgtt cgttgtactg ctgtttgcgc tggctcagac
     6001 ccgccataaa cagtggagtc tggcgaaaaa agtgctggtg ggtctggtga tgggtgtggt
     6061 ttttggcctt gccctgcata ccatttatgg ttctgacagc caggtactta aagattctgt
     6121 acagtggttt aacatcgttg gtaacggcta tgttcaactg ctgcaaatga tcgttatgcc
     6181 gttagtcttc gcctctattc tgagcgcggt tgcccgtctg cataacgcat ctcagttagg
     6241 caaaatcagt tttctgacca tcggtacgct tttgtttacc acgctgattg cggcgctggt
     6301 cggtgtgctg gtcaccaacc tgtttggttt gacggctgaa ggtctggttc agggtggtgc
     6361 agaaactgca cgtctgaacg ccattgaaag taactatgtt ggtaaagtct ctgatctgag
     6421 cgttccgcag ctggtcttgt cctttatccc gaaaaacccg tttgccgatc ttaccggagc
     6481 caatccgacg tcaattatca gcgtggtaat ttttgccgca ttcctcggcg tagctgcatt
     6541 gaaactgctg aaggatgatg cgccgaaagg tgaacgcgtc ttagccgcta tcgataccct
     6601 acaaagctgg gtgatgaaac tggttcgcct tgtcatgcag ttgacccctt acggcgttct
     6661 ggctctaatg accaaagtgg ttgcaggttc taacttgcaa gacatcatca aactgggaag
     6721 tttcgttgtc gcgtcctacc tcggtctgct gattatgttt gcagtgcatg gcattctgct
     6781 gggcattaat ggcgtgagtc cgctgaagta cttccgtaag gtatggcctg tgctgacgtt
     6841 tgcctttacc agccgttcca gtgctgcgtc tatcccactg aatgtggaag cacaaacgcg
     6901 tcgtctgggc gttcctgaat ccatcgccag tttcgccgcc tctttcggtg caaccattgg
     6961 tcagaacggc tgcgccggtt tgtatccggc aatgctggcg gtgatggttg cgcctacggt
     7021 tggcattaac ccgctggacc cgatgtggat tgcgacgctg gtcggtattg ttaccgttag
     7081 ttccgcaggc gttgccggtg tcggtggtgg tgcaactttc gccgcactga ttgtgctgcc
     7141 tgcgatgggc ctgccagtaa ccctggtggc gctgttaatc tccgttgaac cgcttatcga
     7201 catgggccgt acggcgctaa acgttagtgg ctcgatgaca gctggcacgc tgaccagcca
     7261 gtggctgaag caaaccgata aagccattct ggatagcgaa gacgacgccg aactggcaca
     7321 ccgttaattc ttatgctggc aatggtccgt tttgtatagg ggccgttgct ttacttttta
     7381 aatgttccat cgcggcgatg catactgact catatttaaa cagatcgtca gcgaa
//
LOCUS       DAJTMC010000186         7225 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.186, whole genome shotgun sequence.
ACCESSION   DAJTMC010000186 DAJTMC010000000
VERSION     DAJTMC010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7225
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.186"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            600..884
                     /locus_tag="OO130_003643"
     CDS             600..884
                     /locus_tag="OO130_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCR5366900.1"
                     /translation="MIYGGFMKSGVQLNLRARESQRILIDAAAEILHKSRTDFILEMA
                     CKAAEDVILDRRVFNFNDRQYEEFIEMLDAPVADDPAIEKLLARKPQWDV"
     gene            872..1357
                     /locus_tag="OO130_003644"
     CDS             872..1357
                     /locus_tag="OO130_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5366901.1"
                     /translation="MGCVTAPEPLSSFHQVAEFVSGEAVLDDWLKQKGLKNQAIGATR
                     TFVVCRKGTQQIVGFYSLATGSVNHTEATGNLRRNMPDPIPVIILARLAVDVSFRGKG
                     LGADLLHDAVRRCYRVAENIGVRAIMVHALTENAKQFYIHHGFKPSKTQVQTLFLKLP
                     Q"
     gene            1391..2569
                     /gene="ltrA"
                     /locus_tag="OO130_003645"
     CDS             1391..2569
                     /gene="ltrA"
                     /locus_tag="OO130_003645"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCR5366902.1"
                     /translation="MCVATREPPGCDGQTLKMFDQQRDGNLYKIWNRLCSGTWFPPPV
                     LEKRIPKSNGKERILGIPTVSDRIAQGAIKLFMEEKLDPIFHADSYGYRPGKSAHDAL
                     KQCAIRCWRYSWILEVDISAFFDHVKHDLVLKALEHHGMPKWVILYCRRWMEAPMQSC
                     ENGELITRTRGTPQGGVISPLLANLFLHYAFDLWMEREYRGVPFERYADDIVVHCSRM
                     SDATRLKNRLSERFSEVGLVLNAGKTNIAYIDTFKRRNVATSFTFLGYDFKVRTLKNF
                     KGERYRKCMPGASNAAMRKITETIKKWRIHRSTAESLLDFARRYNTIVRGWIEYYGKF
                     WSRNFNYRLWSAMQSRLLKWMQSKYRLSNRKAQRKLTLVRKEYPKLFVHWYLLRASNE
                     "
     gene            2652..2806
                     /locus_tag="OO130_003646"
                     /pseudo
     CDS             2652..2806
                     /locus_tag="OO130_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2860..3342)
                     /locus_tag="OO130_003647"
                     /pseudo
     CDS             complement(2860..3342)
                     /locus_tag="OO130_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016154415.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(3598..4578)
                     /locus_tag="OO130_003648"
     CDS             complement(3598..4578)
                     /locus_tag="OO130_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HCR5366903.1"
                     /translation="MENRKHRPTIGRTLNTNILNNTEEISAPVHVFTLNTGRKAKFTE
                     IKVDHDKVDTQTFVVEEVNGREQTALTPDSLKDITRTIRLQQFYPCIGIRTGDLIEIL
                     DGSRRRAAALLCKVGLRVLVTDDELTVSEAQHLAKDLQTSLEHNIREIGLRLVRLKEA
                     GMNQKQIAEREGLSAAKVTRALQAASVPKDFVSLFPVQSELTYADYRQLAELSERLRL
                     GDISIDEVVKNISPSIELITADDNLSEDEVKNSIMRLITKEMSSLLDSGVKDKAVVTL
                     LWKFDSKDKFARKRVKGRTFSYEFGRLPLEVQDKLDRMIALVLKDNLNSL"
     gene            complement(4578..5777)
                     /locus_tag="OO130_003649"
     CDS             complement(4578..5777)
                     /locus_tag="OO130_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR5366904.1"
                     /translation="MTSFEQLSKVAQRADKMLLALTKQIQEQKQEFQADVFYQVYSKS
                     AVAKLPKLTRASVDGAVGEMEAQGYQFEKRPAGTATKYALTIQNIIDIYAHRGIPKYR
                     DRYSEAYSIFIGSLKGGVSKTVSSVSVAHALRAHPHLLSEDLRILLLDLDPQSSATMF
                     LNYLHAVGLVDTTAPQAMLQNVSREELLEDFIVPSVIPGVYVMPASIDDAFIASNWDT
                     LCEEHLLGQNKHAILRENIIDKLKHDFDFILIDTGPHLDAFLKNAIAAADIMFTPVPP
                     AQVDFHSTLKYLARLPELVQIIEQDGCSCRLQANIGFMSKLANKSDHKYCHSLTKEIF
                     GGDMLDVSMPRLDGFERSGESFDTVISANPVTYVGSGEALKNARMAAEDFAKAVFDRI
                     EFIRANY"
     gene            complement(6099..6437)
                     /locus_tag="OO130_003650"
                     /pseudo
     CDS             complement(6099..6437)
                     /locus_tag="OO130_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(6504..>7225)
                     /gene="istB"
                     /locus_tag="OO130_003651"
     CDS             complement(6504..>7225)
                     /gene="istB"
                     /locus_tag="OO130_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS21-like element IS100kyp family helper ATPase
                     IstB"
                     /protein_id="HCR5366905.1"
                     /translation="LISAAPALSQQAVDQEWSYMDFLEHLLHEEKLARHQRKQAMYTR
                     MAAFPAVKTFEEYDFTFATGAPQKQLQSLRSLSFIERNENIVLLGPSGVGKTHLAIAM
                     GYEAVRAGIQVRFTTAADLLLQLSTAQRQGRYKTTLQRGVMAPRLLIIDEIGYLPFSQ
                     KEAKLFFQVIAKRYEKSAMILTSNLPFGQWDQTFAGDAALTSAMLGRILHHSHVVQIK
                     GESYRLRQKRKAGVIAEANPE"
BASE COUNT         1921 a         1646 c         1731 g         1927 t
ORIGIN      
        1 ggcggcatcc ggcatatagg cagcatgaac gcaacacagg ctctcgtacg gaacgtggga
       61 acctgtcgtc tcgatgctaa gggagcggcg cggagcagag gcactgcaag cgcctgagta
      121 ccgatgcgag acacaggggc agatggcttc gtagtagtga ggaagtgcct gtaatgggca
      181 tggagcgaag ggggtcagtc gaggcgtttg aggttaccaa tcgaagctat acccggtaat
      241 ggcgcaggtt gacctgcact tggccaagtg gattgcccgc aaacacaagc gagcgcgagg
      301 tagtctggtg cgtgcattcg agtggttact gcgaattcgt cacgactgcc cgacgctgtt
      361 tgcacactgg tgcttagctt acgatacttg aatggttcaa cgacaagagc cgtatgacgc
      421 gagagtgtca cgtacggttc cgtgggagcg taggggggcg gtgcccctgc gccacccgat
      481 atcccctttg agtgcggtta cgtccacaga ggtaaatgcc gctgacgcgg catctgtaat
      541 ctgttacttt gtgtacatca tttgctatat attgtcaata tgctattgtt gtaaagcaaa
      601 tgatatacgg aggctttatg aaatcaggtg ttcagctcaa tctcagagca agagaatcac
      661 agcggatact cattgatgct gctgcagaaa ttcttcataa gtcgcgtaca gatttcattc
      721 tggaaatggc ctgtaaagct gctgaagatg ttattctcga ccggcgggtg tttaatttta
      781 atgacaggca atatgaagag ttcattgaaa tgcttgatgc tccggttgca gatgatcctg
      841 ccatcgaaaa attgctggca aggaaacctc aatgggatgt gtaactgcac cagaaccttt
      901 atcctcattt catcaggtcg ctgaatttgt cagcggtgaa gctgtacttg atgactggtt
      961 aaagcagaag gggctaaaaa atcaggcaat cggagccacc cggacttttg ttgtatgcag
     1021 aaaaggtacg caacaaatag tcggttttta ctcactggcc actggtagtg tcaatcatac
     1081 agaagctaca ggtaaccttc ggcgcaatat gccagacccc attcctgtaa ttatacttgc
     1141 acgcctggct gtagatgtgt catttcgagg aaaagggctt ggcgcggatt tacttcatga
     1201 tgcagttcgt cgttgttaca gggttgccga aaatatcggt gtgcgtgcaa ttatggttca
     1261 tgcgcttacg gaaaacgcaa aacagtttta tattcatcat ggttttaagc catcaaaaac
     1321 tcaggtgcaa acattgtttc ttaaactccc tcaataggga gtaatgcact ggtctgggca
     1381 tcatatctgg atgtgcgtcg caacaaggga gccccccgga tgcgacgggc agactttgaa
     1441 aatgttcgac cagcaacgtg atggcaatct atacaagatc tggaaccggc tatgttcggg
     1501 aacatggttt ccgccaccag tgctggaaaa acggatcccg aagtcgaatg gcaaggaacg
     1561 cattctgggg atcccaacag tatccgatcg aattgctcag ggagcgataa aacttttcat
     1621 ggaagaaaag cttgacccga ttttccatgc tgattcatat ggttaccgac caggcaaatc
     1681 agctcatgac gctctgaaac aatgtgccat ccggtgctgg cgttatagct ggatactgga
     1741 agtggacatc agtgcgtttt tcgaccatgt gaaacatgat ctggtgctca aagctctgga
     1801 acaccacggg atgcctaaat gggtcatcct gtactgtcga cgctggatgg aagcgccaat
     1861 gcagagttgt gaaaatggag aattaataac ccgaacacga ggcactccgc agggcggggt
     1921 cattagtccg ctacttgcta acttgttcct tcactatgca tttgatttgt ggatggaaag
     1981 agaatatcgg ggggtaccgt ttgagaggta cgctgacgat attgtagtac attgttcacg
     2041 aatgagtgat gcaacaaggc taaagaacag gttatcggag cgcttctcgg aagtcgggct
     2101 ggtcctgaat gcagggaaaa cgaatatcgc ctacattgac acgtttaaaa ggagaaacgt
     2161 cgcaacgagt ttcacctttc tcggatatga cttcaaagtg cgtacgctga agaatttcaa
     2221 aggcgaacgg taccgaaaat gcatgccggg tgcgtcaaat gcagcaatgc gcaaaataac
     2281 agaaacaatc aagaagtggc gtatacatcg ctcaacagct gagagtttgc tggattttgc
     2341 gaggcgctac aatacgatag tgagaggctg gatcgagtac tacggaaagt tctggtccag
     2401 aaatttcaac tatcgactgt ggagtgcaat gcagtcacgt ctgctcaagt ggatgcagtc
     2461 taaatacaga ctttcgaacc ggaaggctca gcgaaagctg acgctggtaa ggaaggagta
     2521 tccgaagcta tttgttcact ggtatttact acgtgcatca aatgagtgat caagagccgt
     2581 atgacgcgag agtgtcacgt acggttccgt gagcagccgg ggagtgaaat tctctccggc
     2641 tgactcgact cgcgggttcc gacagcgatg acgaacatgc ggcggtgttg tactcgctga
     2701 tcggcacatg ccgtctgaac aatgtggaac cagaaaatgg ctgcgttacg tcattgagca
     2761 tatccaggac tggccggcaa accgggtacg tgatctgttg ccctagaaag ttgatctgag
     2821 ctctcagtaa atatcaatac ggttctgacg agccgcttac ttttggcgta attctgctgc
     2881 gcctttactg aacacaatgc cgtattcagc aagaagtcca cgaatgtgat ttatcagttg
     2941 tgtcctttcc attgaaacca accgacgcat attcaaaaaa gtttggacgt cctgttgctc
     3001 cagagttttt attggtacgg gtcgtatagt tccatgttgg caggcctcag ctattgcctg
     3061 tgcgtcgtta taatcatttt tctggcaccg ctgataagct ttaacatgtt gtgccggaag
     3121 caatattaca tcatggtcga gtttcctgat ttcccgggcc cagtaatgag atgccccgcc
     3181 agcctccatc gcaacagtca ttttaggctg ttgcaccagc cagttaagca gttgattgcg
     3241 agaaagtttg gtctttcctg cgggtttgcc atgagcattg atactgaaaa gataaaagtt
     3301 cgttttagcc agatcaatgc cggtgatttt ctgttcagac atggtatccc gcttactttt
     3361 gtggttaaag tttatccatc atggcgcaat tgaaggctgc cgtgaaagag ggggaagact
     3421 atctcatcgg ttactgttaa acggctaaga gaaactatct ttttcaagca aagaagatac
     3481 ggtgaaacag gcgatttcac cgtgaaatac tgttcaaaaa aaacgctaat aaaagcaaaa
     3541 tttcttttaa aacagccaat taaaaactgg tgagttgctg gcgaaaaact tgaattttca
     3601 cagcgaattc agattatctt ttagtacaag agcaatcata cgatctaact tatcctgaac
     3661 ttccaacggt aatctgccga attcatatga gaacgtacgt ccctttactc gttttcgagc
     3721 aaatttatct ttactatcaa atttccataa caaagttaca accgctttat cctttactcc
     3781 tgaatcaagc aatgaagaca tttccttggt tataagccgc ataatactgt ttttaacctc
     3841 gtcttcagaa agattgtcat cagcagtaat aagctcaata ctcggcgaga tattcttcac
     3901 aacttcatca atagatatat cacccagcct cagtcgttca cttaattcag ccagttgcct
     3961 gtaatcggcg tatgtcagct cagactgaac aggaaaaaga gacacaaaat ctttaggtac
     4021 ggacgctgct tggagggcac gagtcacctt agctgccgac aatccctctc tttctgcaat
     4081 ttgcttctgg ttcataccgg cctctttgag acgtactaaa cgcaacccaa tttcacgaat
     4141 attatgttca agtgacgtct gcaaatcttt cgccagatgc tgagcttcag aaacagtaag
     4201 ctcatcatcc gttacaagta cacgtaatcc aaccttgcat agcaaagctg ctgcccgacg
     4261 acgggaacca tccaaaatct caataagatc gccagtccga atcccaatac atggatagaa
     4321 ttgttgtaaa cgtatagttc tggtgatgtc ttttagtgag tccggcgtca gggctgtctg
     4381 ttcgcggcca ttcacctctt caaccacaaa agtttgagtg tccactttgt catgatcaac
     4441 cttgatctca gtaaattttg cctttcggcc agtattgagg gtaaatacat gaactggagc
     4501 cgatatttct tcagtattat tcaggatatt tgtattcagc gtacggccaa tagtcggtcg
     4561 gtgctttctg ttttccatca gtagttggcc cttataaatt caatacgatc aaaaactgct
     4621 ttagcgaaat cttctgctgc catacgtgca tttttcaaag cttcaccact gccaacataa
     4681 gtaacaggat tcgcagatat cacggtatca aacgactcgc cgcttctctc aaaaccatca
     4741 agacgtggca ttgaaacatc aagcatgtca ccaccgaaaa tctcttttgt caggctatgg
     4801 cagtatttat gatctgactt attcgccagt ttcgacatga agccaatatt tgcctgcaaa
     4861 cggcatgaac atccatcctg ttcaataatc tgtacaagtt caggtaaacg agccaaatat
     4921 ttaagcgtgg aatggaaatc aacttgtgct ggcggcaccg gtgtaaacat gatatctgcg
     4981 gcagcaatgg catttttcaa aaatgcgtcc agatgcgggc ctgtatcaat aaggatgaag
     5041 tcgaaatcat gcttcagctt atcaataata ttctcacgca atatcgcgtg tttgttttgt
     5101 cctaatagat gctcctcgca cagagtatcc cagtttgatg caataaaggc gtcgtcaata
     5161 gaagccggca taacataaac gccagggata actgacggaa cgataaaatc ttccagtaat
     5221 tcctcacgac tcacgttctg caacatagcc tgcggtgcgg ttgtatcaac cagccccacc
     5281 gcatgcaagt agttcagaaa catagtggct gatgactggg gatctaaatc aaggagcaga
     5341 atacgtaaat cttctgacag caggtgagga tgagcacgca gggcgtgagc tacggaaaca
     5401 ctactcacgg ttttagacac accgcctttt agactaccga tgaatatgga atatgcctca
     5461 ctataacgat cgcgatattt aggaatgcca cgatgtgcat agatatcgat aatgttctgg
     5521 atggttaatg catattttgt cgcggttcct gctgggcgtt tttcaaactg atagccctga
     5581 gcctccatct caccgactgc accatccacg cttgcgcgcg ttaattttgg aagcttagct
     5641 acagcagact ttgaatagac ttgataaaaa acatcagcct gaaactcctg cttttgctcc
     5701 tgaatctgtt ttgttagggc gagcaacatt ttatctgcac gctgcgcaac cttacttaac
     5761 tgctcaaaac tagtcattcc ccctccgtgc tatgtaggtt tttaacttat tacaaaaaaa
     5821 actgcattag cagggtaatg catgcaagtg catattgtac atgcttatat taaaaatgcg
     5881 cgcattgctg atttattaat catcggaatg aatattactg taagatagta tgcctttccg
     5941 ggagaaagac gctgatttcc cccgaataat ccagttcacc accacactcc ggacaacagc
     6001 tttcttcagg ctccagatgg tgtatttcgc gggaaacatg ttccggtaac ggacggcgat
     6061 gacgtgactg tcgcaacagg cgggggtagt gcttccaatc agtagaacat gtgcttttca
     6121 atgaaggagc cgatgacttt ttcgtggatt tccacggaac gcgagaagca gattgtttta
     6181 cgtgccagcc gcttgatgcg ggtgcggagg gtccggttgt tacgttcaat acgttgcgtg
     6241 aagattttgc cggtcaggtg tttctccttc ggcacttctc tggcgtagct gccccagtca
     6301 tcgctggtca ccatgccgat acagaacgga gtcaacaggg ctaacagttc ccgacaggtc
     6361 tcatcattcc gtggcccgaa agtgtaagcc aacacaccgc cagttttagt gttgtacgca
     6421 taccagagcc agtgttgtgt caacgacgga tgaaaagtga tccacttata tctccaccaa
     6481 cggcccaata ttgatccacc gttttactca ggattagctt ctgctataac cccggccttt
     6541 cgtttctgtc tgagtcgata gctttctcct ttgatttgaa cgacatgtga gtggtgtaag
     6601 atacggccca gcatcgctga ggtcagtgct gcatcaccgg cgaacgtttg atcccactgc
     6661 ccgaacggta gattggatgt caggatcatt gcgctctttt cataacgttt agcgatgacc
     6721 tggaagaaca gttttgcttc tttctgactg aacggcagat agcctatttc atcaatgatg
     6781 agcaggcggg gggccattac tccacgctga agcgtcgttt tataacggcc ctgacgttgt
     6841 gccgtagata actgaagtaa cagatctgct gctgttgtga agcgaacttg gatacctgca
     6901 cggactgctt catagcccat cgcgattgcc agatgggttt tccccacacc tgatggcccc
     6961 agtaatacga tattttcatt acgttctatg aagctgagtg agcgtaacga ctggagttgc
     7021 ttctgcggtg ctccggtggc gaatgtgaag tcatactctt cgaacgtttt caccgccggg
     7081 aaggctgcca ttcgggtata catcgcctgt ttacgttgat gacgtgccag tttttcttca
     7141 tgaagcagat gctccaggaa gtccatataa ctccattcct ggtctactgc ctgttgtgac
     7201 agcgcaggcg ctgcgcttat aaggc
//
LOCUS       DAJTMC010000187         7214 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.187, whole genome shotgun sequence.
ACCESSION   DAJTMC010000187 DAJTMC010000000
VERSION     DAJTMC010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7214
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.187"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(826..1356)
                     /locus_tag="OO130_003652"
                     /pseudo
     CDS             complement(826..1356)
                     /locus_tag="OO130_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416483.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
     gene            complement(1699..2349)
                     /gene="zinT"
                     /locus_tag="OO130_003653"
     CDS             complement(1699..2349)
                     /gene="zinT"
                     /locus_tag="OO130_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HCR5366906.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDANVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYHKGYA
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            complement(2606..3241)
                     /gene="msrQ"
                     /locus_tag="OO130_003654"
     CDS             complement(2606..3241)
                     /gene="msrQ"
                     /locus_tag="OO130_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HCR5366907.1"
                     /translation="MRLTAKQVTWLKVSLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLATLLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLRSLFNRLRKQVHNKLSV"
     gene            complement(3242..4246)
                     /gene="msrP"
                     /locus_tag="OO130_003655"
     CDS             complement(3242..4246)
                     /gene="msrP"
                     /locus_tag="OO130_003655"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HCR5366908.1"
                     /translation="MKKNQFLKESDVTAESVFFMKRRQVLKALGISAAALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKPLEFSKPAAWQNNLPLTPVDKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYADQVASLYRGLDLRENF
                     "
     gene            complement(4355..4768)
                     /gene="hiuH"
                     /locus_tag="OO130_003656"
     CDS             complement(4355..4768)
                     /gene="hiuH"
                     /locus_tag="OO130_003656"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HCR5366909.1"
                     /translation="MLKRYLVLSVATAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            4901..5572
                     /gene="hprR"
                     /locus_tag="OO130_003657"
     CDS             4901..5572
                     /gene="hprR"
                     /locus_tag="OO130_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HCR5366910.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLRMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            5572..6930
                     /gene="hprS"
                     /locus_tag="OO130_003658"
     CDS             5572..6930
                     /gene="hprS"
                     /locus_tag="OO130_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HCR5366911.1"
                     /translation="MKRLSITVRLTLLFILLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIVLCSVLSPLLIRTGLREIKKLSGVTEALNYNDSREPVEVSALPRELKPL
                     GQALNKMHQALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDTNGYLNIDIASLGTK
                     IHEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSASYHYLNKHNVFRITLPQ
                     RN"
     gene            complement(7038..>7214)
                     /locus_tag="OO130_003659"
     CDS             complement(7038..>7214)
                     /locus_tag="OO130_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310732.1"
                     /note="Hsp31 stabilizes early unfolding protein
                     intermediates under severe heat stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HCR5366912.1"
                     /translation="HLTWYFGEELKKMGMNIINDDITGRVHKDRKLLTGDSPFAANAL
                     GKLAAQEMLAAYAG"
BASE COUNT         2025 a         1490 c         1632 g         2067 t
ORIGIN      
        1 gctgagaaaa aagcgtgctt tcccaatctg gggcaaatca cgtctgaatg acacctgaaa
       61 acaggtggtg gactcaagaa ggcttggaat attcttcttt aatgttatgt aattcattga
      121 tttttcgtgt acgattttaa gacatttttt ccaagaaaaa tttttaactc tttgattttt
      181 cgactctgta tcatcggtct cgaaaaccgg agtaggggca actctaccgg gggggcaaat
      241 ccccctctct ccgccacttt atcaatgact tatctcccga cttcccgcct tacttttcct
      301 aaacagaaca atcgtagaat attcttgaag ggttagatcg tcactgtttt ctgttcgata
      361 ctgtgacatt cagcacttga ttcgctatgg atctgacagg aagctttcga gcgaaaatgt
      421 gcagttattc agtcgttttc ttatcgctca ccattattct tttagacatt catcctatga
      481 aactgccgca aagttggtgg tgggaactga agttgcgtag agaaggggtc aataattgag
      541 tcaaaaaagg aacgcggccg tcagattaaa gccgcgggac aaagtccata atttccaaga
      601 gttgttgaat ttcccagctt ttttgaaagc ctttgtatca tactaacaag taaaaaaggt
      661 ttaaatacca aaattaacct gtatttttga taagtcttac cattgtacga tttacacagt
      721 ttctgctcct ccttgttgat agtggaaagg gtgcattgtg cacttgcgta aacatttatg
      781 acgcgttgat gtccatggtt cacatttcct tgtcagggga tcgacttatt tgcggcgagg
      841 catcattcgt aacagagtat tatctttctg aatgtagtga tgaaagagtg ctgcgccagc
      901 gtgagctgcg attaaaaagt aacctatatt tgcccaggtt tcgtgaatat tttatatcag
      961 tgctttaatt tcgctgtttg gggtaacaaa gggagacaca ttgaaaccaa ggaaactcca
     1021 cgattttcca ctgtaagcca tcaaagcaat ccccagcaga ggaagtgcaa ggaaggttat
     1081 ataaagcatg atgtgcatta gcgaagccgc tttcatttgc caggcaggtg gcggtggcac
     1141 aatagaagga tcactataac ggtgttttat aatcaggcgt gaaaacatta acacccaaac
     1201 gaatataccc gcattgtaat gtgtttctcg catcagcaga taagtactac tacctttagg
     1261 aaaccagcca cggagttcca tcgcggcata ggttattgca acgaaaagca gcgttatcca
     1321 atgtaaataa atttgagttt ttgagaatcg gttcattttt gtagctcctg taatttttac
     1381 agtcaagcta tcccacgaac cttaaaaatt ccttatacag atgtggtttt aagaatttgt
     1441 tatttaaaaa attgatagtg cacattgtaa ccagccaata taagcaatgg cgtgagcaca
     1501 ggtatctttc tctgttggcc gtattgtgta tggaatccgt tattggttaa ctcaatgcgg
     1561 gttagcttat taatatacag acacgtcata gtgatgagcg tgtctgtagc tagcttgcgt
     1621 tcagtggcgt ttgtggatgg accagtggca gttatgatga cttactaaag cggcatcgag
     1681 gcgttatcat gagaatactc aatgagacat catttcctcg accacttctt cgctactcaa
     1741 ctgatatgga taatacgttg gccagttttc catttcattt aataaagatt gctgtgaatc
     1801 attacccata aaaatgtgaa aatgagatga ttttcgtggt gcaattatat gatcgctaaa
     1861 ttgtatatat ttaggggctt tgctttcagg atctttacat tcgaataagt aacgaacgcc
     1921 tttcttgcct gatttatagg tgagtatttt gtatccatcg taatcatatt tacaggatgt
     1981 tgtttcatta tttctatgga attcaacaat gccgtcctca atgccaatca tctcgatatc
     2041 tgttgcataa cctttgtgat aataatcttt aatttcagca aatgttttag ttttatctgc
     2101 atccgctttc ttctgaaaga cggggtcaag tttgccactt tgcagtaaag gataaacgga
     2161 ttgccagacc ccatcccagt cactgagcgt tcggttttgt acattggcat catcaaaaac
     2221 accattagca gctttttgtt cgacctctgt taagggtttg ccgtgtgagt gatgaccatg
     2281 cgaaaaggca ggagcgctaa caataaagac acctaacgca acagccagtt tgtgaagacg
     2341 aatcgccaaa acagtttcct ccagagtcat tgtatatgat gtgtaatgtt atattgtaac
     2401 atatggcaaa atgcaacaac tcttaggaga tgagagcata gttttctcag gtgtacttat
     2461 ctatcttatt gaaataaaat gaattatatt gataatttga ggttgatgac aaacgaaaca
     2521 taatctgatg tgatctgcgt aggccggata gggcgctcgc gccgcatccg gcagggataa
     2581 cgcgtacttt gtcggcaata tgaagttata cagataattt gttgtgaacc tgcttgcgta
     2641 accggttaaa aagggaacgc aacttcttat accgtaaggc taaaagcagt acagccagcc
     2701 cagcgtagat gagcggctgc ggtgaaataa tcttcactga ccacagataa tgtattgggg
     2761 ccaggatcgc gacaagatag acgaaattat gcaactgttg ccagtgcttg ccgagttttc
     2821 gctgcatcgc ctgggttgaa gtaaacgcta aagcaagcaa aataacccag ctgataattc
     2881 ctaacgttaa ataaggccgg gtaattaact ccttgcctaa taacgccaga ttattcacgc
     2941 ccagctccag caatgcgtaa ctggttaaat gcagtgtcgc ccaggcaaag caccataagc
     3001 ctaacaggcg gcgagtgcgt atcaataacg gctgttttgc gtagcgtgcc agaggtgtga
     3061 ttaacaaggt cgccagcaaa aatttcagtg cagtgcgacc ggtaaaatgc tgaatatctt
     3121 tcaccggatc ggcacccagt ccaccgtgat tgatcgccca gaccagccag agaaacggca
     3181 acaatccggc aagatgcagg ctaactttca gccatgtcac ctgttttgct gtcagacgca
     3241 ttcagaaatt ctcccgcaaa tccaggccac gatacagcga tgccacctgg tcggcgtaac
     3301 cattaaacag tagcgttggc tggcgctgaa catcgaggat gccgcctgaa ccaataaatc
     3361 gttcggtagc ctgtgaccag cgcgggtgat caacatgcgg attaacgttg gcgtaaaaac
     3421 cgtattcgtc aggcgctgcc agattccagg tggttggcgg acgctcgcgg gtcagcttaa
     3481 tactgactat cgatttaatc cctttaaagc catatttcca cggcacaatc agtcgtaccg
     3541 gcgcgccatt ttgtggcggt aacgccttgc cataaacacc cacggtcatc agtgtgagcg
     3601 gatgcattgc ttcgtcgaga cgcaatcctt cgacataagg atatttcagc ccgccgccga
     3661 taaagcggtc ctgctggcct ggcatttgtt ccggtgcata aattgtttcg aaagcgacat
     3721 acttcgcatt gctggtgggt tcggcaagcg ccagcaattt gtgcagcgga aaaccaatcc
     3781 acggcaccac catcgaccat gcctccacgc agcgcatacg ataaatacgc tcttccagcg
     3841 ggaagcgacg ggttaaatca tcgtgatcga gggtcaatgg ttttgccact tcgccgctga
     3901 ttttcagtgt ccatggatcg gttttcaggc taccagcatt agcggcggga tcggctttat
     3961 ccagcccgaa ttcatagaag ttgttataac cggagacttt atctactggc gtcagtggca
     4021 ggttattttg ccaggcggca ggcttgctga actccagcgg ttttccggcg ggggcgggcg
     4081 ggcgatcgtt ccctttaaac cagctaagca gatcggcatg cgcagcgtga ggcaaagaaa
     4141 gtgcagctgc actgatgccc agtgctttta acacctgccg acgcttcata aagaataccg
     4201 actcggccgt aacatctgat tcttttaaaa attgattctt tttcataagc catcacactt
     4261 ttttgtcgtt agccagcaag agccgtttac tgttaatctg tcagggagtg tcctccctaa
     4321 cagtagcaga gatctgcttt tacatattta tgcattaact gccacgatag gttgaatacc
     4381 catattggct aagtaataaa ggcacatgat aatgctcgtt cactttatta atatgaaatt
     4441 caacggggat ctcagggaag aaactttcaa gattttgttt cttgaaatag tccccggttt
     4501 taaatacgac acggtaatcg cccgtagttg cagtttgctc gggccacagt gctttaattc
     4561 gtccatcctt atctgttttg gcggtattaa gttgtaacca gccgttatcc gccttctttt
     4621 caagagtgac tgtcacgtca gcagcgggct ttccggtttg ctggttcaaa atgtgcacgc
     4681 taagaatgtt ttgttgtgcg gcataaacca aagaaggtaa tgaaaatgct gccgttgcta
     4741 ccgagagtac taaataacgc tttaacatgt ttatatcctt gtcatgtgaa tgagttttga
     4801 ggatagcgaa aaagcggcca gggtaacctt acagcagcat tacaattttg taatgaacca
     4861 ggctgtttct ataacatatg atttatggca tattattttc atgaagattt tacttattga
     4921 agataatcaa aggacccagg agtgggtaac gcaggggctt tccgaagcgg gttatgtcat
     4981 tgatgccgtt tctgatggca gagatgggct ttatcttgcg ctgaaggatg attatgcatt
     5041 gataattctg gatattatgc ttccgggtat ggatggctgg cagatcttac aaacgttaag
     5101 aacagcaaag caaacccctg ttatttgcct tactgcaagg gattctgtcg atgacagagt
     5161 cagagggctg gacagtggag caaatgatta tctggtaaaa cctttttcat tttctgagtt
     5221 gctggcaagg gttcgggcac aattaaggca acatcacgct ttgaattcaa cattagaaat
     5281 cagtggctta agaatggact ctgtaagtca aagtgtgagc agggacaata tcagtattac
     5341 actgacgcgc aaggagtttc agttactttg gctactggcc tccagagctg gcgaaattat
     5401 acccagaacg gttattgcga gtgaaatttg gggaatcaac tttgatagtg ataccaatac
     5461 ggtggatgtc gccattcgca ggctccgcgc aaaagttgat gatccttttc ctgaaaagct
     5521 aatcgccaca atccggggga tgggctattc attcgtagcg gtaaaaaaat aatgaaaaga
     5581 ttgtctataa ccgtccgttt aaccttgctt tttatattgc tgctgtctgt tgctggcgct
     5641 ggaattgtct ggactctcta taatggcctg gcaagtgagt tgaaatggcg cgatgataca
     5701 acactcatta accggacagc acagatcaag cagttgttaa ttgatggggt aaatccagat
     5761 acgttacctg tatactttaa ccggatgatg gatgttagtc aggatatctt gatcattcat
     5821 ggtgatggca tcaataaaat tgttaaccgg acaaatgtca gtgatgacat gttaaataac
     5881 atacctgcta gtgagacaat cagcgcagct ggcatttaca gaagcattat taatgatact
     5941 gagattgatg ctttacgaat taatattgat gaagtttcgc catcattaac ggttactgtg
     6001 gctaaattgg cttcagctag acataacatg cttgaacagt ataaaattaa tagcattata
     6061 atttgcattg tcgccattgt actttgctca gtattaagtc cgctgttaat cagaacggga
     6121 ttacgagaga tcaaaaagtt gagtggtgta acggaagcgc tgaattataa cgatagccga
     6181 gagcctgttg aggttagcgc attaccgaga gaactaaaac ctcttgggca ggcgttgaat
     6241 aaaatgcatc aagccttagt caaagatttt gaacgcctaa gtcaatttgc tgacgatctc
     6301 gctcatgaac ttagaacgcc cattaatgca ttactgggtc agaatcaggt tacgctcagt
     6361 caaaccagaa gtatcgctga atatcaaaaa acaattgccg gtaacattga agagttggaa
     6421 aatatttcgc ggttaacaga gaacatactg tttcttgccc gggcagataa aaacaatgtt
     6481 ttggtgaaac tggactcgct ttctctcaat aaggaagtcg aaaatttgtt ggattatctt
     6541 gaatatcttt cagacgagaa agagatttgc tttaaggtcg agtgcaatca gcaaatcttt
     6601 gcggataaaa ttttactgca acgaatgtta tcgaatctta ttgttaatgc cattagatat
     6661 tcaccagaaa aatcgcgtat tcatataacc agttttcttg ataccaacgg ctatcttaat
     6721 attgatatcg ccagtcttgg aacgaaaata catgagcctg aaaaactctt ccgtagattt
     6781 tggcggggag ataattcgcg tcattccgta ggtcagggac ttggtctttc tttagtcaaa
     6841 gcgattgccg aattgcatgg gggaagtgct tcatatcact atctcaataa gcataatgtg
     6901 ttccgaatta cgttaccgca aagaaattaa catagggctt cagtacgcca taaattaacc
     6961 gtgaaaaaac gcagccacgt cgtgagtact aacgcggctg cgattaatta tgcgcttaca
     7021 ttcaaacgta acagggatta acccgcgtaa gctgccagca tttcctgcgc cgccagttta
     7081 cccaacgcat tcgctgcgaa aggactgtcg ccggtgagaa gtttacggtc cttatgtact
     7141 cgcccggtga tgtcgtcatt aatgatgttc atgcccattt tcttcagctc ttcgccgaag
     7201 taccaggtga gatg
//
LOCUS       DAJTMC010000188         7199 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.188, whole genome shotgun sequence.
ACCESSION   DAJTMC010000188 DAJTMC010000000
VERSION     DAJTMC010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7199
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.188"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(235..789)
                     /locus_tag="OO130_003660"
                     /pseudo
     CDS             complement(235..789)
                     /locus_tag="OO130_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3H family transposase"
     gene            932..1054
                     /locus_tag="OO130_003661"
                     /pseudo
     CDS             932..1054
                     /locus_tag="OO130_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1316..1882)
                     /gene="ipgB2"
                     /locus_tag="OO130_003662"
     CDS             complement(1316..1882)
                     /gene="ipgB2"
                     /locus_tag="OO130_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB2"
                     /protein_id="HCR5366913.1"
                     /translation="MLGTSFNNFGISLSHKRYFSGKVDEIIRCTMGKRIVKISSTKIN
                     TSILSSVSEQIGENITDWKNDEKKVYVSRVVNQCIDKFCAEHSRKIGDNLRKQIFKQV
                     EKDYHISLDINAAQSSINHLVSGSSYFKKKMDELCEGMNRSVKNDTTSNVANLISDQF
                     FEKNVQYIDLKKLRGNMSDYITNLESPF"
     gene            complement(2164..2478)
                     /locus_tag="OO130_003663"
     CDS             complement(2164..2478)
                     /locus_tag="OO130_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5366914.1"
                     /translation="MLQRQRGKVGFAQLPVDFVAIEPDSAQGVGKRANLTNRCFIIRI
                     NDSFKKRQGFIEFISNSGSGHTVTVYTKRRFQRGVFMNSLNTNVVKPVMYRLRSSADA
                     RP"
     gene            complement(2556..2666)
                     /locus_tag="OO130_003664"
                     /pseudo
     CDS             complement(2556..2666)
                     /locus_tag="OO130_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(2990..3667)
                     /gene="ospD1"
                     /locus_tag="OO130_003665"
     CDS             complement(2990..3667)
                     /gene="ospD1"
                     /locus_tag="OO130_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector/anti-activator
                     OspD1"
                     /protein_id="HCR5366915.1"
                     /translation="MSINNYGLHPANNKNMHLIIGSNTANENKGMKRNIINVTNTAIS
                     HAINEEKSGGGYSGVSFRKLAKIQSISIPTKNNKEYNRHNLFSLIWHGNADAARKYGE
                     SLLAAEIPKEEKLEVLAARNNAGESALFIALQEGHSAAIQAYGDFIKTFDLSPKETIK
                     LLDVRDNEGVPGLFLAAGKGNIEAMMAYINICHHSGIKLTEIADRLNNNEQDMFNIIS
                     DKIQELF"
     gene            complement(4038..4175)
                     /locus_tag="OO130_003666"
                     /pseudo
     CDS             complement(4038..4175)
                     /locus_tag="OO130_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(4257..4555)
                     /locus_tag="OO130_003667"
                     /pseudo
     CDS             complement(4257..4555)
                     /locus_tag="OO130_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343747.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(5144..5303)
                     /locus_tag="OO130_003668"
                     /pseudo
     CDS             complement(5144..5303)
                     /locus_tag="OO130_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(5307..6461)
                     /locus_tag="OO130_003669"
                     /pseudo
     CDS             complement(5307..6461)
                     /locus_tag="OO130_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(6481..6828)
                     /gene="tnpB"
                     /locus_tag="OO130_003670"
     CDS             complement(6481..6828)
                     /gene="tnpB"
                     /locus_tag="OO130_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR5366916.1"
                     /translation="MISFPAGSRIWLVAGITDMRNGFNGLASKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTRRLERGRFVWPVTRDGKVHLTPAQLSMLLEGIDW
                     KHPKRTERAGIRI"
     gene            complement(6828..>7199)
                     /locus_tag="OO130_003671"
     CDS             complement(6828..>7199)
                     /locus_tag="OO130_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5366917.1"
                     /translation="RPNFPYEFKIALVEQSLQPGACVAQIARENGINDNLLFNWRHQY
                     RKGGLLPSGKNMPALLPVTLTPEPDNKIPAPAQEPEQINTPSDSLCCELVLPAGTLRL
                     KGKLTPALLQILIREIKGSSH"
BASE COUNT         1856 a         1676 c         1557 g         2110 t
ORIGIN      
        1 aaaccttctc atgtttagag ttttttatta tgtttatcat aatgatatga cgatctgagt
       61 ttttaaaatc aagaagaaaa gataagtttt ttaaatgcac agtgcctgtg gataactttg
      121 cgtaaatccg gaggacagat cactctgaag caagacaaca gcatctgaaa acaatatgct
      181 cacgtttcaa ccacagggcg taagtgatcc tacccacgta atatggacac aggcctaagc
      241 gaggttctgg ttttcaaatt gttccggact gaggccgcca caccaactgt gccgtcgcca
      301 gcgattgtaa tcacattcga tataattaaa cactgttgcc cgcattattt cccggctgat
      361 aaagtgttct ccgtggagac attccacttt cagcgaatga aagaagcttt ccacgcaggc
      421 attatcgtag cagcagccct ttgcgctcat actgccacgc agattatgcc acttcagcag
      481 cgcctgataa tctgctgaac agtactgact tcctcggtcc gagtgaacga taacgttccg
      541 gggtctctta cgccgccaca gcgccatctg cagggcatcg caggccagtt gcgccgtcag
      601 gcgtggtgac atggaccagc caataacggc acgtgaccac aggtcaatga ccacagccag
      661 atacagccag ccttcatctg tacgtaagta cgtgatgtct cccgcccact tctggttcgg
      721 accactggcg taaaaatcct gctccagcag attttctgac acaggcaggc catgtgcgcg
      781 gtaactgacc gtattgctgg gtaagatcaa tttgctcact ggtatttact acgtgcatcg
      841 aatgagtgat caagagccgt atgacgcgag agtgtcacgt acggttccgt gagcagccgg
      901 agagtgaaac tctctccggc tgactcgact cgcaggttcc gacagcggtg acgaacatgc
      961 agcggtgttg ttctcgctga tcggcacatg ccgtctgaac aatgtggagc cagaaaaatg
     1021 gctgcgttac gttattgaac atatccagga ctgaccggca aaccgggtac gcgatctgtt
     1081 gccttggaaa gttgatctga cttctcagta aatatcaata tgattctgac gagccgctta
     1141 cctttgagca gagaaagggg agagggatcc cacatccatg agctaggaaa atacaaaaac
     1201 tggctataca acaattcaat atattaggac ttacttgcga ggagaaatat agcaccgctc
     1261 aggcaattcc tgggtattta cttttcttag tttctttact cgggatgttc tgtcatcaga
     1321 aaggcgattc taaatttgta atataatcac tcatattccc acgtaatttc tttagatcaa
     1381 tatattgcac atttttctca aaaaactgat cggaaataag atttgcaaca ttgcttgttg
     1441 tatcattttt aacgcttcta ttcatccctt cacaaagttc gtccattttc tttttgaaat
     1501 aactgcttcc tgatacaaga tgatttatgg aactttgtgc agcattgatg tctagagaaa
     1561 tgtgataatc tttctcaacc tgtttaaata tctgtttacg gagattatcg ccgatttttc
     1621 ggctgtgttc tgcacaaaac ttatcaatac attggtttac cacccgggat acgtagactt
     1681 ttttttcatc atttttccaa tctgtaatat tttcaccgat ttgttctgaa acagagctca
     1741 aaatactagt atttattttg gttgatgata tttttactat ccttttcccc attgtacagc
     1801 ggataatctc atcaaccttt cctgaaaaat atcttttgtg agataaactg attccaaaat
     1861 tattaaaaga tgttccaagc attataatca ctttgtcatg taaaatagta acattgtcat
     1921 tattttagct gattcaaaca attctgtaaa tgctttgagt tctttactcc cttggctcaa
     1981 tgatggaccg tctggaatcc cagagactaa actctctgag aaagagagta tgaaaaaact
     2041 tggggcagtc cctaaatgaa gggaccaaca gcggtaaaat aaccggcatc attcataatg
     2101 atgccctgtt agttgtagtt atgaagattt acgtcattaa aattgctgtt cgtagtgtca
     2161 gtcctatggt ctggcatcag ctgaggatcg tagccgatac atcactggct tcacgacatt
     2221 cgtgttgagg ctattcatga aaactccacg ctgaaaacgc ctttttgtat aaacggtcac
     2281 ggtatgcccg gagccactgt tgctgatgaa ttcgataaaa ccttggcgtt tcttgaagct
     2341 atcgttaatg cggataatga aacaacgatt ggtgagattc gctcttttgc cgacaccctg
     2401 ggcgctgtcc ggttcaatcg caacaaaatc aaccggcagc tgagcaaacc caaccttgcc
     2461 tctctggcgc tggagcatga agtgatttag ctgggacgta gccactgaca gcttctataa
     2521 aagtatgatc ctacccacgt aatatgaaca caggcttaag tgaggttctg gttttcaaat
     2581 tgttcaggac tgagacctcc acaccaactg tgccgtcgtc agcgattgta atcgcacttg
     2641 atataattaa acactgtata tttcatttgt ggagtctcca gtctgagggg gctttatgca
     2701 ttccgtattg ggcactatga tgccagaaat ctgcaaggct ccacaccttc ctgtatttcc
     2761 cttcctctat cacagtgcac tttacttaat agggtgggat gtgttcatta catttattgt
     2821 gttttttatg attgtttttg cgtccagtgt cgttcgtcat gggaaactgg ttctatgggt
     2881 agtatcgctc agtctagtta actgtttgtt tctttttaga aattaagcag atcacacaat
     2941 tcggggagag ctccttctaa gtgcaattct ttgcagctat ttagcacact taaaacaact
     3001 cttgtatttt gtcagaaata atattaaaca tgtcttgttc attattgtta agtctgtctg
     3061 ctatttctgt aagttttatc ccactatgat ggcatatatt tatatatgcc atcatagcct
     3121 cgatattccc tttccctgcg gccagaaata atcctggtac cccctcatta tctcttacat
     3181 ccaatagttt aatcgtttct tttggtgata aatcaaaagt tttaataaaa tctccataag
     3241 cttgaatcgc agcggaatga ccttcttgaa gagctatgaa caaagcagat tccccagcat
     3301 tatttcgtgc tgcaagaact tctagttttt cctctttggg tatttcggct gccaacagcg
     3361 attcaccgta tttacgcgct gcatcagcat ttccatgcca aatcaatgaa aacaaattat
     3421 ggcggttata ctccttatta ttctttgtcg gaatggatat gctctgtatt ttggccaatt
     3481 ttctgaaaga aacaccacta tatccccccc ctgatttttc ttcattgatg gcgtgggata
     3541 tagcggtatt tgtcacgtta atgatattac gtttcattcc tttattttca ttagcagtat
     3601 tgctgcctat tattaggtgc atatttttgt tgtttgctgg atgtaatcca tagttattta
     3661 ttgacatttt tgttcatcct ttttgctaaa aaaaacaata acctattttt gtaatgtaat
     3721 agatattagt ctattgctaa tctaaaaaaa ccattgccac attgaatggc tatattcacc
     3781 aataaattct ttcgatttcg tatattgaca tatcgtagat atatcttatc cctaagattc
     3841 aataatcgag tccgtttacc attctgtgca agcgccttca ctcagaagtg accgtcaagg
     3901 cgatcaccaa acttgataat aaagcagctc attgccatac gccagtcctt aaggggcgtt
     3961 gtccatttct gtgatgctgc ctagatttaa ctgcctgctc cggcttatct acatctaaat
     4021 atgtgagcct aataccgcta cactttggcc agactatgtt tttctccggg aagtgggcta
     4081 accgtctcca taaagtgacg aacttttttc aacagtggcc atattgaacg gcactgatga
     4141 ttacgggtta ttgtctcatg aagagccggc cacatcactg acagcagtga cttcaggcct
     4201 tcagcaccag acagcctgaa ccagttaatc catcgcccga cagatttagt aatctagggc
     4261 tggtttttct tcagctgttg cggttcgaaa gtgccattac gatcacgcag agtacggatt
     4321 tccagaggtt catctccagt agtgataatc ttcgttaagc aaccattgcg ggaattgctg
     4381 ccagatctaa gctgattttt ctcgcaccca agatgatgag tcatattagc attgagtacg
     4441 gtttcatttt cagcatccaa tcaaactgac tgagatcttc aggatttttg agacttttgg
     4501 ctagttcatt agctaaagcc cgcaacttat tagatgtcaa ctgtttttca ttcataaatt
     4561 caccttcatt tgatatcaaa ttaaatagtt caacatcaga gaattataca aacttatgta
     4621 caggctccta tgatcttcga atcctgaaga ttatagtgaa aataagtttt cacgttgaaa
     4681 tcccacatag ttagtatatg ataaataatc atggatgacc tgattaacct cttggggtag
     4741 tgcttttata gaagaataaa tattctacta atttaataca tcacctaaaa tagtattgtt
     4801 ttttatggtt ggttgaaggt cgtggtgatg atgttagtcc ttttgagaat attccagtgc
     4861 cagatcttac ttataataaa ggggcttggc agtttggata aactattgtt cttattccac
     4921 taatacaaac tttttgtaaa gtatattaaa tatacttcat ttcatactga aatccccgga
     4981 agtattcaat caaacaatta caccataaaa ggaagagggg gccctaatta agttgtcaag
     5041 catgtttatg atacctgcag ggtataaatt ttctggctgc aagcagacac atataaaacg
     5101 tctttattga tcctacccac ataatatgga cacagtcgta agtgaggctc tggttttcaa
     5161 attgttccgg actgagacca ccacaccaac ggtgccgtcg ccagcgattg taatcgcact
     5221 cgatataact aaacactgtt gcccgcacta tttcccgatg gataatagtg ttctccgtgg
     5281 agacattcca ttttcagcga gtgaattttc atctgttgct gtatccagtc atacagtgac
     5341 tgcatcagtg gcgcggctct ggcttttctt gccgccagac gctgttctgc tgaacatccc
     5401 cggacttctg cctcgatggc atacagttca ccgatacgca gcagggcttc cgttattctg
     5461 ccggattcgt acaacgcccg gtaaccaccg taagcatcgg cctgaagcac tccgctgtaa
     5521 ccggccagat gattctgtgg atggatacct ttccggtccg gtgagtacgc gaaccagacc
     5581 gccggaggca tttccgaacc ggcgttacgg tcatcacgga catagaccca cagccgggca
     5641 gttcttgttt taccactgcc cggctcctgg accggaacgg ggatatcatc cgcatgaact
     5701 ttaccgggca tcagcacata ctggcgcagg acgtcataca gcggctccag cagttcagca
     5761 acggcaccgg tccagcgacc cagtgtggca cggctcagct ccacgccctg acgacggtat
     5821 atttctgact ggcggtataa cggcagatag cctgcatatt ttccagcgac aacatgggcc
     5881 agaagccccg ctccggcata actgcgtgca atgggttttg aaggcaccgg tgcctgcacg
     5941 atatggtcac accggcaaca ggccagtttc ggacgttgtg tttcgataac cttaaaggcg
     6001 ctgctgataa gctccagctg ctctgacaca tcacatccca gagaactgag ttcgccacca
     6061 caggccgggc agcattcctc ttccggacgg atgacccggg tttcacgggg aagtgaggca
     6121 ggtaacggtt tacgggctga agactggcgc aggggagatg gcagtaccgg gtcatattgc
     6181 tcacccagcg tttccgccat ttcttcctga agtgcgctga ttcgctcctg tgcttccagt
     6241 atctgccgtt cggtttttgc gcgaagtttt tctgagcttt aaccgaactg catgcgctgc
     6301 agtttcgcaa ccagcgcctt cagccggttg atttcggaag cataagccgc cacccgctgt
     6361 gagagcaggc ggttgtattt cgccatctgg cggatggtgt cctgttgcgt ctgcaacagt
     6421 gcccgcaggc gggcattctc atgagcaagt gaggtgtcca tatcctcact ttacaacggg
     6481 ttatatgcgg atgccagcgc gttccgttcg tttcgggtgt ttccagtcga tgccctcaag
     6541 aagcatggat aactgggccg gagtaaggtg caccttgccg tcacgggtga ccggccagac
     6601 gaagcggccc cgctccaggc gtctggtaaa gaggcacagt ccgtcactgt cagcccacaa
     6661 cacttttatc tggtcacccc ggcgtccgcg gaagatgaac agatgcccgg agaacgggtc
     6721 atccttcagg acgttctgaa cttttgaggc caggccgtta aagccatttc gcatatcggt
     6781 gatacctgca accagccaga tacgcgaacc tgcagggaaa gatatcatca gtggctgctc
     6841 ccttttattt cgcggataag tatctgtaat aacgccggcg tcagtttacc tttaagcctg
     6901 agagttccgg ccggcagaac cagctcacag cacagactgt cggacggtgt atttatctgc
     6961 tctggttcct gtgccggagc cgggatttta ttatccggct ccggcgttaa cgtcacggga
     7021 agcagtgcag gcatattttt tccggaaggc agcaggccac ctttccggta ttgatggcgc
     7081 cagttgaaga gcaggttatc gttgattccg ttttcacggg cgatctgcgc cacacaggct
     7141 ccgggctgca gtgactgctc cactaaggcg attttaaatt cataaggaaa attaggccg
//
LOCUS       DAJTMC010000189         7120 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.189, whole genome shotgun sequence.
ACCESSION   DAJTMC010000189 DAJTMC010000000
VERSION     DAJTMC010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7120
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.189"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            <1..648
                     /locus_tag="OO130_003672"
     CDS             <1..648
                     /locus_tag="OO130_003672"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HCR5366918.1"
                     /translation="TYKYADMLAMSAKKDAMVPMGGLLCMKDDSFFDVYTECRTLCVV
                     QEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYRIAQVQYLVDGLEEIGVVCQQAGGH
                     AAFVDAGKLLPNIPADQFPAQALACELYKVAGIRAVEIGSFLLGRDPKTGKQLPCPAE
                     LLRLTIPRATYTQTHMDFIIEAFKHVKENAANIKGLTFTYEPKVLRHFTAKLKEV"
     gene            739..1986
                     /gene="tnaB"
                     /locus_tag="OO130_003673"
     CDS             739..1986
                     /gene="tnaB"
                     /locus_tag="OO130_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HCR5366919.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            2118..3293
                     /gene="mdtL"
                     /locus_tag="OO130_003674"
     CDS             2118..3293
                     /gene="mdtL"
                     /locus_tag="OO130_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HCR5366920.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            3268..4227
                     /gene="yidZ"
                     /locus_tag="OO130_003675"
     CDS             3268..4227
                     /gene="yidZ"
                     /locus_tag="OO130_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HCR5366921.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA"
     gene            4384..5133
                     /locus_tag="OO130_003676"
     CDS             4384..5133
                     /locus_tag="OO130_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HCR5366922.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            5155..5721
                     /gene="yieF"
                     /locus_tag="OO130_003677"
                     /gene_synonym="chrR"
     CDS             5155..5721
                     /gene="yieF"
                     /locus_tag="OO130_003677"
                     /gene_synonym="chrR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HCR5366923.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDRQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(5775..7112)
                     /gene="adeP"
                     /locus_tag="OO130_003678"
     CDS             complement(5775..7112)
                     /gene="adeP"
                     /locus_tag="OO130_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HCR5366924.1"
                     /translation="MSQQHTTQASGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQIGMGAIFWGAVGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANSETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
BASE COUNT         1691 a         1823 c         1803 g         1803 t
ORIGIN      
        1 acctacaaat atgccgatat gctggcgatg tccgccaaga aagatgcgat ggtgccgatg
       61 ggcggcttgc tgtgcatgaa agacgacagc ttctttgatg tgtacaccga gtgcagaacc
      121 ctttgcgtgg tgcaggaagg cttcccgaca tatggcggcc tggaaggcgg cgcgatggag
      181 cgtctggcgg taggtctgta tgacggcatg aatctcgact ggctggctta tcgtatcgcg
      241 caggtacagt atctggtcga tggtctggaa gagattggcg ttgtctgcca gcaggcgggc
      301 ggtcacgcgg cattcgttga tgccggtaaa ctgctgccga atatcccggc agaccagttc
      361 ccggcacagg cgctggcgtg cgagctgtat aaagtcgccg gtatccgtgc ggtagaaatt
      421 ggctctttcc tgttaggccg cgatccgaaa accggtaaac aactgccatg cccggctgaa
      481 ctgctgcgtt taaccattcc gcgcgcaaca tatactcaaa cacatatgga cttcattatt
      541 gaagccttta aacatgtgaa agagaacgcg gcgaatatta aaggattaac ctttacctac
      601 gaaccaaaag tattgcgtca cttcaccgca aaactgaaag aagtttaatt aatactacag
      661 agtggctata aggatgttag ccactctctt accctacatc ctcaataaca aaaatagcct
      721 tcctctaaag gtggcatcat gactgatcaa gctgaaaaaa agcactctgc attttggggt
      781 gttatggtta tagcaggtac agtaattggt ggaggtatgt ttgctttacc tgttgatctt
      841 gccggtgcct ggtttttctg gggtgccttt atccttatca ttgcctggtt ttcaatgctt
      901 cattccgggt tattgttatt agaagcaaat ttaaattatc ctgtcggctc cagttttaac
      961 accatcacca aagatttaat cggtaacacc tggaacatta tcagcggtat taccgttgcc
     1021 ttcgttctct atatcctcac ttatgcctat atctctgcta atggtgcgat cattagtgaa
     1081 acgatatcaa tgaatttggg ctatcacgct aatccacgta ttgtcgggat ctgcacagcc
     1141 attttcgttg ccagcgtatt gtggataagt tcgttagccg ccagtcgcat tacctcattg
     1201 ttcctcgggc tgaagattat ctcctttgtg atcgtgtttg gttctttctt cttccaggtc
     1261 gattactcca tcctgcgcga tgccaccagc accactgcgg gaacgtctta cttcccgtat
     1321 atctttatgg ctttgccggt gtgtctggcg tcatttggtt tccacggcaa tattcccagc
     1381 ctgattattt gctatggaaa acgcaaagat aagttaatca aaagcgtggt atttggttcg
     1441 ctgctggcgc tggtgattta tctcttctgg ctctattgca cgatggggaa tattccgcgc
     1501 gaaagcttta aggcgataat ctcctcaggc ggcaacgttg attcgctggt gaaatcgttc
     1561 ctcggcacca aacagcacgg cattatcgag ttttgcctgc tggtgttctc taacttagct
     1621 gttgccagtt cgttctttgg tgtcacgctg gggttgttcg attatctggc ggacctgttt
     1681 aagattgata actcccacgg cgggcgtttc aaaaccgtgc tgttaacctt cctgccacct
     1741 gcgttgttgt atctgatctt cccgaacggc tttatttacg ggatcggcgg tgccgggctg
     1801 tgcgccacca tctgggcggt cattattccc gcagtgcttg caatcaaagc tcgcaagaag
     1861 tttcccaatc agatgttcac ggtctggggc ggcaatctta ttccggcgat tgtcattctc
     1921 tttggtataa ccgtgatttt gtgctggttc ggcaacgtct ttaacgtgtt acctaaattt
     1981 ggctaaatcc ttcaagaagc cagccattcg ctggcttctt gcctctcagg aaatcactta
     2041 tgtccaaatg gcaactcgcc tgatcctcct tcaccacgta tgctttgcgt caccttacta
     2101 tcaggacgct ttagcccatg tcccgctttt tgatttgtag ttttgccctg gttttacttt
     2161 atcccgccgg gattgatatg tacctcgttg gtttaccgcg catcgccgcc gatctcaatg
     2221 ccagcgaagc gcagttgcat attgcgttct ccgtatatct ggcggggatg gcagctgcga
     2281 tgttatttgc cggtaaagtg gccgatcgtt cagggagaaa gccggtcgcc atacccggcg
     2341 cggcgctatt tattattgcc tcggtgttct gttcactggc tgaaaccagc acgttatttc
     2401 ttgcaggccg atttctacag gggttgggcg caggctgttg ttacgtagtg gcgttcgcca
     2461 ttttgcgcga cacgctggat gatcgacgtc gggctaaagt gctgtcatta ctcaacggta
     2521 ttacctgcat cattccggtg ttagcgccag tgctcggaca tctgattatg cttaaattcc
     2581 cgtggcagag tctgttctgg gcgatggcaa tgatgggcat cgcggtactg atgttgtctt
     2641 tgtttatttt aaaagaaacg cgcccagcgt cccccgctgc ttcggacaaa ccacgagaaa
     2701 atagcgagtc gctgcttaat cggtttttcc tcagccgtgt tgttatcacc accctcagcg
     2761 tttcggtgat cctcactttc gtcaatacat cgccggtatt gctgatggaa atcatgggtt
     2821 ttgagcgcgg agaatacgcc accattatgg cgttgaccgc tggcgtcagc atgaccgttt
     2881 cattctccac gccatttgcg ctgggaattt ttaagccacg tacgttgatg atcacctcgc
     2941 aggtgttatt ccttgcagcg gggatcaccc ttgccgtttc accttcccat gcggtttctc
     3001 tgtttggtat cacactgatt tgcgccggtt tctcggtagg ttttggcgta gcgatgagtc
     3061 aggcgttagg accattttca ttacgcgcgg gcgtagccag ctcgacctta ggtattgcgc
     3121 aggtttgcgg ttcgtcactg tggatttggc tggcagcggt ggttggtatc ggcgcatgga
     3181 atatgctgat cgggattctg attgcctgta gcatagtgag cctgttgctg attatgttcg
     3241 tcgcgcctgg acgccccgtt gccgctcatg aagaaatcca tcaccacgct tgatctcaat
     3301 ctgctgctct gtctgcaact gctgatgcag gagcgcagcg tgaccaaagc ggcgaagcgg
     3361 atgaacgtga ccccttcagc ggtgagtaag tcgctggcaa agttaagagc gtggtttgac
     3421 gacccgctct ttgtgaactc accgctgggg ctgtcgccca caccgctaat ggtcagcatg
     3481 gagcaaaatc tggcggaatg gatgcaaatg agcaatctgc tgctggataa accgcaccac
     3541 cagacaccgc gcggcctgaa gtttgagttg gcggcggaat cgccgctgat gatgatcatg
     3601 cttaatgcgc tgtcgaaacg gatctaccaa cgttacccgc aggcgaccat caaactacgt
     3661 aactgggatt acgattcctt agatgccatt actcgtggtg aagtggatat tggtttttcc
     3721 ggtcgcgaaa gccatcctcg ctcgcgggag ctgttaagct cgctaccgtt agccattgat
     3781 tatgaagtgc tgtttagtga tgtaccctgc gtctggttac gcaaagatca tccggcactg
     3841 catgaaacgt ggaatctgga caccttttta cgttatccgc atatcagcat ttgctgggaa
     3901 cagagcgaca cctgggcgct ggacaatgtg ttacaggagc tggggcgcga acgcacgatt
     3961 gctatgagcc tgccggaatt tgagcagtca ctgtttatgg ctgcgcaacc cgacaatctg
     4021 ctactggcga ccgcgccgcg ctactgtcag tactacaatc aactccatca actgccgttg
     4081 gttgctcttc ctctcccgtt tgacgaaagc cagcaaaaaa agctggaagt tccttttacc
     4141 ctgctgtggc ataaacggaa cagtcataat ccgaagatcg tctggttacg ggaaaccatt
     4201 aaaaaccttt acgcgtcgat ggcataaccg aatcgtatga aacgcgaccc aatttcacaa
     4261 taaaatgtaa aaaagttgta ataagcttgt ctgaatcgaa cttttagccg ctttagtctg
     4321 tccatcattc cagtaaatga ttactcttat attcataatg gaccattaag catggagcga
     4381 aaaatggcga ctcactttgc ccgagggatt ttaacggaag gacatctgat ttctgttcgt
     4441 ctcccctccc agtgtcatca agaagcccga aacattcccc ctcatcgtca aagccgtttt
     4501 ctggcgtcca gaggtttact cgcagaactg atgttcatgc tgtatggcat tggcgaattg
     4561 ccggaaatcg tcaccctgcc gaaaggtaaa ccggttttca gtgataaaaa tttgccttcg
     4621 ttttctattt cctatgccgg gaatatggtt ggcgtggcgt taacaaccga aggtgaatgt
     4681 ggcctcgata tggaactaca gcgtgcgacg cgcgggtttc atagcccaca cgcgcccgat
     4741 aaccacacct tttccagcaa tgaatcgcta tggatcagta aacaaaacga tcctaacgaa
     4801 gcgcgggcgc agctcatcac gctgcgccga agcgtgctaa aactaaccgg tgatgttttg
     4861 aatgacgatc cgcgcgatct gcagctgctg cccattgccg gacgcctgaa atgtgctcat
     4921 gtaaatcatg tagaagcgtt atgcgacgcg gaggacgtgc tggtgtggtc cgtggcggtc
     4981 acgcccgcga ttgaaaagct cagtgtctgg gagttagatg gcaaacacag ctggaaaagc
     5041 ctgccggata ttcacagccg cgccaacaat cctaccagcc ggatgatgcg ttttgcccag
     5101 ctctctaccg tgaagtcttt ttcgccaaat tgatagacaa ccacaggagt cattatgtct
     5161 gaaaaattgc aggtggttac gttactgggg agcctgcgca aaggctcatt taatggcatg
     5221 gttgcacgta ccctgccgaa aattgctccg gcgagcatgg aagtcaatgc gttaccttcc
     5281 attgccgaca ttcccctgta tgacgctgac gtacagcagg aagaaggttt tccagcaacg
     5341 gttgaagctc tggcggaaca gatccgtcag gctgacggtg tggtgatcgt cacgccggaa
     5401 tataactact cggtaccggg tgggctgaaa aatgccatcg actggctttc ccgcctgccg
     5461 gatcaaccgc tggccggtaa accggtgttg attcagacca gctcaatggg cgtgattggc
     5521 ggcgcgcgct gtcagtatca cctgcgccag attctggttt tcctcgatgc aatggtgatg
     5581 aacaagccgg aatttatggg cggcgtgatt cagaacaaag ttgatcggca aaccggagaa
     5641 gtgattgatc agggtacgct ggaccacctg accgggcaat tgaccgcatt tggtgagttt
     5701 attcagcgag ttaagatcta aataaaaaac ccgccagtaa tcatgcatgg cgggttttta
     5761 atgcgctatc gattttaatg agcgtcgata aatacaatct tcaggataaa cagcagcgca
     5821 acgatgatta cgcacgggct aaggtcacgc agacgcccgg taccaatctt catcacgcag
     5881 taggagataa agcccagcgc aataccttcg gtaatcgaaa agctgaacgg catcatcacg
     5941 gcggtaataa acgccggaac agattcagta agatcctgcc agttcacgcg agcaagactt
     6001 gaggtcatca acacgccaac gtagatcaac gcgccagctg cagcgtagcc tggcaccatt
     6061 cccgccagcg gcgacagaaa gataaccagc aggaacagca gaccaacaac cactgccgtc
     6121 agaccggtac gaccgccaac cgatacgccg gaagaggact caatataagc cgtaacggaa
     6181 gaagtaccga taaacgaacc ggtcacggaa gagatactgt cgacatacag cgcctgcttc
     6241 atgcgcggga atttcccctt ctcatccgcc agacctgctt tatcggtcac gccaatcagc
     6301 gtaccggagg agtcaaacaa gttgaccaac atgaaagaga aaatcacccc tgccagcccg
     6361 aggttaaacg acccggctaa atctacatga cccacaactg tcattacgct cggcggcgca
     6421 gaaacgatgc cattgtagtg cacatcaccc agcatccagc ccagcagcgt cgtcaccacg
     6481 atagaaacca gcaccgctgc gtgaatgttg cgcgaggcca gaatagcaat gatgaagaag
     6541 ccgaggatac ccagaagtac gctgtgagaa gtcagattac cgatgctcac cagcgtttcc
     6601 gagttagcga caatcacacc tgcgtttttc agccccatca tgccgataaa caggccgata
     6661 ccgctggtaa tacccacacg cagacttacc ggaatgttag caatcatcca gtaacgaacg
     6721 cggaaaatgg tcaaaagaag cagtccgaca gcgccccaga agattgcgcc cataccgatc
     6781 tgccacggca gccccattgc ctggacaacc acaaaagcga aaaaggcgtt aagccccatc
     6841 gcaggagcca gcgccaccgg aaggtttgca aacaatccca tcataatgct accgaaagca
     6901 gcaatcagac aggttgttac gaagacggca ctggtgtcca tgccagcaac gcccagaatt
     6961 tgtgggttaa caaaaacgat gtaaaccatc gtcaggaagg tggtaaaacc ggcgatcact
     7021 tcggtccgtg ccgtcgtgcc atgttcgcgc agtttaaaca cgcgttccag catcccctgg
     7081 ccagaagcct gggttgtgtg ttgttgactc attatctatt
//
LOCUS       DAJTMC010000190         7077 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.190, whole genome shotgun sequence.
ACCESSION   DAJTMC010000190 DAJTMC010000000
VERSION     DAJTMC010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7077
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.190"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            <1..332
                     /locus_tag="OO130_003679"
     CDS             <1..332
                     /locus_tag="OO130_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCR5366925.1"
                     /translation="ELLMKVQLGGGTNIASAVEYGRQLIEQPAKSVIILVSDFYEGGS
                     SSLLTHQVKKCVQSGIKVLGLAALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASW
                     LAENLQS"
     gene            329..2329
                     /locus_tag="OO130_003680"
     CDS             329..2329
                     /locus_tag="OO130_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HCR5366926.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     GALIATFSDGVRTQLANGQALKEAQCSCGASGMCRHRVMLVLSYQRLCATAQPTGKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKGITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVQAKAQQAELTHLIWQMRSEHVTSSDDPFASEEG
                     KTCRQYVQQLSQALWLSGISQPLIHYEAAFSRAQQAAERCSWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMARRDSVGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQENSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSGRQTLDAQVISGEGEDNLLTLSL
                     PASACSPFAVERMAALLQQTDDPVSLVSGFVSFVEGQLTLEPRVMMTKTRAWALDAET
                     APVAPLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAISQAELLANDLTAV
                     GFYRLAHVLVQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            2454..2915
                     /locus_tag="OO130_003681"
     CDS             2454..2915
                     /locus_tag="OO130_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HCR5366927.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASISATTKEDAAKTLEPLSAKYKNIAGVEEKLTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(2956..3426)
                     /locus_tag="OO130_003682"
     CDS             complement(2956..3426)
                     /locus_tag="OO130_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HCR5366928.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(3473..4192)
                     /gene="yehT"
                     /locus_tag="OO130_003683"
     CDS             complement(3473..4192)
                     /gene="yehT"
                     /locus_tag="OO130_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HCR5366929.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(4189..5874)
                     /gene="btsS"
                     /locus_tag="OO130_003684"
     CDS             complement(4189..5874)
                     /gene="btsS"
                     /locus_tag="OO130_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HCR5366930.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLQLPWRDEA"
     gene            6096..6161
                     /locus_tag="OO130_003685"
                     /pseudo
     CDS             6096..6161
                     /locus_tag="OO130_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
     gene            6389..6637
                     /locus_tag="OO130_003686"
     CDS             6389..6637
                     /locus_tag="OO130_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HCR5366931.1"
                     /translation="MRVEICIAKEKITKMPNGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            complement(6753..7022)
                     /locus_tag="OO130_003687"
     CDS             complement(6753..7022)
                     /locus_tag="OO130_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR5366932.1"
                     /translation="MLLQHSDIEIAWYASIQQEPNGWKTVTTQFYIQEFSEHIAPLQD
                     AVDLEIATEEENSLLEAWKKYRVLLNCVDTSTAPDIEWPEEPDTM"
BASE COUNT         1809 a         1806 c         1724 g         1738 t
ORIGIN      
        1 tagagttatt aatgaaagta cagttgggcg gcgggaccaa tatcgccagt gccgtggagt
       61 atggtcggca acttattgaa caaccagcga aaagcgtcat tatcctcgtg agcgattttt
      121 acgaaggggg ttcatcatca ttactgacgc atcaggtgaa aaagtgtgtc cagagcggca
      181 tcaaagtgct gggactggca gcgctcgata gcacagcaac accttgctat gaccgcgata
      241 cggcccaggc gctggttaat gtcggcgcac aaatagccgc catgacaccg ggcgagctgg
      301 catcatggct tgcggagaat cttcagtcat gaattcacta cgtccggaat tattagaact
      361 gacaccgcag gccctgacgg cgttaagcaa tgccggtttt gttaagcgca gtcttaagga
      421 actggaaaat ggcaacgtcc cggagatcag ccatgagaac ggcgctttaa tcgccacctt
      481 cagtgacggt gtccgtaccc agctggcgaa cggccaggca ctgaaagagg ctcagtgcag
      541 ctgcggggcc agcggtatgt gccgtcatcg cgtgatgctg gtgttaagtt atcaacgact
      601 ttgtgctacc gcccagccta cgggaaaaga agaggaatgg gatccggcaa tctggctgga
      661 agaactggct acccttcccg atgccacccg caaacgcgca caggcgctgg tcgctaaagg
      721 catcaccatt gagttgttct gtgcgccggg tgaaattccc tctgcccgct taccgatgag
      781 cgatgtgcgt ttttattccc gcagcagtat tcgtttcgcc cgttgtgatt gtattgaagg
      841 cacactttgc gaacatgtcg tactggcagt acaggccttt gtccaggcaa aagcgcagca
      901 agcggaattg actcatttaa tctggcagat gcgcagcgaa cacgtcacat catctgacga
      961 tccgtttgcc agcgaagaag gcaaaacgtg tcgtcaatat gttcaacaac taagccaggc
     1021 attatggctg agcggcatca gccagccgct tattcactac gaggccgctt tcagccgcgc
     1081 gcagcaagca gcggaacgct gcagctggcg atgggtgagt gaatcgctac ggcaactgcg
     1141 cgccagcgtt gatgccttcc acgcccgcgc cagccactat catgccggag aatgcttacg
     1201 tcagcttgcg gcattaaaca gtcgattaaa ctgcgcacaa gagatggccc ggcgcgacag
     1261 tgttggtgaa gttcctcctg tgccgtggcg cacggtcgtt ggctctggca ttgccggaga
     1321 agcaaagctt gatcatctgc ggctggtgtc tttaggtatg cgttgctggc aggatattga
     1381 gcattatggt ttacgcatct ggtttaccga tcccgacacc ggcagtattt tgcatctttc
     1441 gcgtagttgg ccgcgaagtg aacaggaaaa ctcaccggca gctacgcgtc ggctgtttag
     1501 ttttcaggct ggcgcactgg cgggtgggca aattgtttca caagcagcaa aacgcagtgc
     1561 cgatggcgag ctgctgttag ctacccgcaa ccgcttaagc agcgttgtgc cgctgtcgcc
     1621 agacgcctgg caaatgttga gcgcgccgtt acgccagccg ggcattgtgg ctttgcggga
     1681 atatttacgc cagcgtcccc ccgcctgcat acggcctctt aatcaggtgg ataacttatt
     1741 tattctgccg gtcgctgagt gtatttcact cggttgggac agcggccgcc agacgctgga
     1801 tgcgcaggta atcagcggcg aaggtgaaga taatctgctg acgttatccc ttccggcatc
     1861 tgcctgttct ccttttgccg ttgaacgcat ggcggcgctt ttgcaacaaa cagacgaccc
     1921 cgtgagtctg gtttctggct ttgtcagctt tgtagagggg caattgacac tggaaccacg
     1981 ggtgatgatg acaaaaaccc gtgcctgggc gctggacgcg gaaactgcgc ctgtggcacc
     2041 gctaccttct gccagcgttt tgcctgtgcc gtctaccgct catcagttgc tgatgcgctg
     2101 ccaggcgtta cttattcaac tgctccataa cggctggcgc tatcaggaac agagcgctat
     2161 tagtcaggca gagttgctgg cgaatgacct caccgcggtg ggtttttatc ggctggcaca
     2221 tgtgttggta caatttcgta atacagaaag cgaggcacgg gtagaagcaa tgaataacgg
     2281 tgttttgctt tgcgaacaat tattccccat gcttcagcaa caaggatgaa atagtgcttt
     2341 ttactaagag ttctactcca gttccggact gctcacgcca cggtattagg catatcctat
     2401 atagcccctg gtgagagtca ccagtttctt gattaaataa aatggagttt tacatgaagg
     2461 ctttcaataa gctgttttcc ctcgttgttg catctgttct ggttttctct cttgctggct
     2521 gcggtgacaa agaagaatcg aagaaattca gcgccaatct gaacggcact gaaattgcca
     2581 ttacctatgt ctacaaaggt gacaaggtgc ttaagcaatc ttctgaaacc aaaattcaat
     2641 ttgcctccat tagtgcaacc accaaagaag atgctgccaa gacacttgag ccgttaagcg
     2701 ccaaatacaa aaacatcgcg ggtgttgaag aaaaattaac ctataccgat acctacgcgc
     2761 aggaaaacgt gactatcgat atggaaaaag tggattttaa agccctgcag ggtatttcag
     2821 gaatcaacgt ttctgctgaa gatgccaaaa aaggtatcac tatggcgcaa atggaactgg
     2881 tgatgaaagc cgctggtttt aaagaagtga aataatctgt cggcggccat gtttcgtatg
     2941 gccgccatac ccgtcttagc ttttcttcac atgctggcgc gctgccagtc cacgcagaaa
     3001 ataacgtaaa aattgatcgc cgcattcgcg gaagttttta tgatccggtg cgcgcatcat
     3061 cgctgtaatt tccggcatcg aaacgcggaa ctgctgttcg gtgaggatag ccagaatgtc
     3121 atcggttttc agcgaaaacg cgatgcgtaa ttttttcagc acgatgttgt tattaatgcg
     3181 acgttccggc tccagtgccg gagcagactc atccttgccg cgtttttcat aaatcaggcc
     3241 attgaggaat gacgacaaaa caatgtccgg acaacgctga aaaccctctt cgtcttcttt
     3301 acgtagccag acggcgatct gttccgcggt ggcttcgaca ttacccagcg ccagaatacg
     3361 caccaggtca ttattattgg ctttcaaaat gtagcgcacg ctgcgcagaa tatcgttact
     3421 tagcatgagg ccttcaggtg ttgatgaggc aaaaagccat tttagcagtc ttttacaggc
     3481 caatcgcctc ttttaagctt ttcagataac ggcggctgac cggcacggtt aagccattac
     3541 gcaaaatcaa ctcggcctgg ccgttatctt ccagacgaat ctcctgtaaa tgcgcgaggt
     3601 taaccagata ctgacgatgg cagcgcagta gtggtgtacg actttccagg gtacgtaatg
     3661 tcaattcggt aaagccctct ttcccttcgt ggctggtaac gtagacaccg ctcatccgac
     3721 tgctgacaaa tgccacatct ttcatttgca gcaaataaat ccgactatgc cccgtacaag
     3781 ggataaattt cagcgcctgt tgattttccg gtaacagcga aacatcctgc ttgctgcgct
     3841 cctgacgcaa tcgcgccagc gttttctcca gtcgcgcttc atcaattggc ttcagcagat
     3901 aatcaaaggc atgttcttca aaggctttaa tagcgtattc gtcaaacgca gtgagaaaaa
     3961 caatatacgg gcgatgttcc gggtcaagca tccccaccat ttccagacca ctgatgcgcg
     4021 gcatctggat atcgagaaac agcacatccg ggcgcagttt atgcaccgcg ccgatccctt
     4081 ccacggcgtt tgaacactct ccaacgattt caatatcgct ctgctcctgc aaaaatacac
     4141 gcaggttctc ccgtgctaac ggttcatcat cgacaattaa gactttaatc atgcctcgtc
     4201 cctccatggt agttgtaacg ttattcgggt gtaactatca ggctcacagg cgacgcttat
     4261 tccatagtca tcgccaaacc gttcacgtaa acgcttatcc accagattca tccccagccc
     4321 actggcattg gttaccggtt gatacaaacc ggcattgtct tcgatctcca gcatcaaatg
     4381 ttgcccctca cgtcgggcgc tgattgccac tcgccctgta tccagcagtt gtgatgtccc
     4441 atgtttaatg gcgttttcca ctatcggttg cagggtaaac gcgggcaatt gctgctggga
     4501 taattcttgc ggaatagcaa tgttgacctg caaccgcgac tggaagcgcg ccttttcaat
     4561 ttgcagataa gcgttcacat gttcaatttc gtcggcgaga gtaacaaact ccgaaggccg
     4621 ctttaagttt ttgcggaaaa aagtggaaag atactgcacc agctggctgg cctgttcgct
     4681 gtcgcggcgg atcaccgctt taatggtgtt aagcgcatta aacaaaaaat gaggattcac
     4741 ctgggcgtga agcagtttga tttctgactg ggtgagcatc gctttttgcc gctcatattg
     4801 cccggcaaga atctgcgccg aaagcagttg cgcaatcccc tcgcccagcg tgcggtttat
     4861 tgaactgaat aaacggtttt tggcttcata caatttgatg gtgcccatca cccgctgatt
     4921 ttcaccacgc aacggaatta ccagcgtcga ccccagtttg cattgcggat gcaaagagca
     4981 acgataaggt acttcgttgc catcagcgta gaccacttca ccggtttcaa tcgcttttaa
     5041 ggtgtaagtc gaagaaatcg gtttgccggg taaatggtgg tcgtcaccaa ttccagtaaa
     5101 ggccagcaat ttctctcgat cggtaatcgc gactgcacca atatccagct cctgatacag
     5161 cacctgtgcc actttcatgc tgttcacttc gttaaacccc tggcgcaaaa tgccttccgt
     5221 cgaggcagcc actttcagcg cagtggcaga aaaagccgaa gtgtattttt caaacatcgc
     5281 gcgtttatcg agcaatatac gcataaacag cgccgcgccg acggtattgg tgaccatcat
     5341 tggtgcagca atattactca ccagacgcac cgcatcttca taaggtcggg cgatcgccag
     5401 gatgatcaac atttgcacca tttcagcgac gaacgtgacg gcaccggcgg taatggggtt
     5461 aaagacttta tcagtacgcc cgcgacgaat caggatgctg tgtaccaggc cgccgagcaa
     5521 tccttcaacg atggtcgaga tcatgcaact tagcgcggtc atgcccccca tcgaatatcg
     5581 atgtaacccg ccggtcaggc caaccagccc accgacgacc ggaccgccga gtaagccgcc
     5641 cattaccgcg cctatcgcac gggtattggc aatagaatcg tcaatgtgca acccaaacca
     5701 ggtgcccatg atgcagaaga tggaaaagac gatgtagcag agaaatttat gcggcagacg
     5761 aaccgtgacc tgcattaacg gtatgaataa tggcgtttta ctcattaacc acgcaatgac
     5821 taaaaaaacg cacatctgct gaagcagcag caacaccaga ttaaaatcgt acatacccgc
     5881 aaaccacact tccctttaaa acgcgtaaca tacattgcct gcgtttaact ttctttgaac
     5941 tcttgcagaa aaatgagaat tcgtgagtac gatcactcaa aatcgcctgg caaaaataaa
     6001 atcaccctat agatgcacaa aaaacgggca aaactacctg gttcgcaaaa ctgcgtctaa
     6061 agttaaaccg ggacctcgcg agcaagggtg agacgatggc gctttacaca attggtgaag
     6121 tggcgttgct ttgtgatatt aaccctgtca cgttacgcgc gtgacgcaac attttaaaaa
     6181 atcgacgaag atcgtccagg agcgccgttt ttcaagggtt ggatagatga ttcgtttacc
     6241 atgtcgacga acttcgacga atttcgccga taaaaagtgt cccatacatg ccccattgat
     6301 gcaccataaa caatatcaga aattgatact ggttatccat acagataaaa ataacacctg
     6361 tacataacta cagttacagg agggtgttat gcgtgttgag atctgtatcg ctaaagaaaa
     6421 aatcactaaa atgccaaacg gtgctgtgga tgcgttaaag gaagaattaa cccgacgcat
     6481 cagtaaacgt tatgacgatg tagaggtgat cgtaaaagcc accagcaacg atggcctttc
     6541 tgttacacgc accgcagata aggattctgc aaaaacattt gttcaggaga ctctgaaaga
     6601 tacctgggag tctgctgacg agtggtttgt tcactaataa acacgtaaaa tcggtaacgg
     6661 ctggaaatca ttcaatactc gcactatcga aagtttacca gccaaccgca gcacgtcctg
     6721 catacgtcgt gtctacggtt tttctttttc gcttacattg tgtctggttc ttccggccac
     6781 tcaatatcag gtgcagttga tgtatcaaca cagttcagca acacccgata cttcttccag
     6841 gcttccagca acgagttttc ttcctccgtt gcgatctcca gatctacagc atcctgaagt
     6901 ggcgcaatat gctcactgaa ttcctggatg tagaactgtg tggtgacggt cttccagcca
     6961 ttcggctcct gctgtatcga agcataccag gctatttcaa tatcgctatg ctgcagcagc
     7021 atttaacccc ttgtaattca tcgccataat tgatttaatt cacaaataaa actataa
//
LOCUS       DAJTMC010000191         7056 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.191, whole genome shotgun sequence.
ACCESSION   DAJTMC010000191 DAJTMC010000000
VERSION     DAJTMC010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7056
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.191"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            40..246
                     /locus_tag="OO130_003688"
                     /pseudo
     CDS             40..246
                     /locus_tag="OO130_003688"
                     /inference="COORDINATES: protein motif:HMM:TIGR02601.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            991..2904
                     /locus_tag="OO130_003689"
                     /pseudo
     CDS             991..2904
                     /locus_tag="OO130_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001306477.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(3032..3754)
                     /locus_tag="OO130_003690"
     CDS             complement(3032..3754)
                     /locus_tag="OO130_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-demethylubiquinone
                     3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol
                     methylase"
                     /protein_id="HCR5366933.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     LTNTFKLGPGVDVNYMLHTQNK"
     gene            3901..6528
                     /gene="gyrA"
                     /locus_tag="OO130_003691"
     CDS             3901..6528
                     /gene="gyrA"
                     /locus_tag="OO130_003691"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HCR5366934.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDAAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            6677..>7056
                     /locus_tag="OO130_003692"
     CDS             6677..>7056
                     /locus_tag="OO130_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 family protein"
                     /protein_id="HCR5366935.1"
                     /translation="MSGEKKAKGWRFYGLVGFGAIVLLSAGVWALQYAGSGPEKTLSP
                     LVMHNNLQIDLNEPDLFLDSDSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIE
                     GSIRRIVYEHDLTLKDKLFSSLLD"
BASE COUNT         1693 a         1743 c         2014 g         1606 t
ORIGIN      
        1 ggctatggag atggtccttc cactgcggcg ggtggcttta tgtatctcgg cttaagtgaa
       61 gttacctttg atattgccga cggaaaaacg ctggttatcg gcaatacaga gaatgacgga
      121 gctgttgact ctattgctgg taccgggttg atcaccaaaa caggttccgg cgatctggta
      181 cttaatgcag ataacaatga ctttactggc gagatgcaga ttgaaaacgg tgaagttacc
      241 ctgggccgca gcaactccct gatgaatgtc ggcgatacgc attgccagga cgatccgcaa
      301 gactgctacg gtctgacgat agggagtatt gataagtacc agaatcaggc agagctaaac
      361 gttggctcca cccaacaaac ctttgtgcac tcattgacgg gctttcagaa tggcacttta
      421 aatatcgatg ctggtggcaa tgttactgtt aatcaaggta gttttgctgg caccatcgaa
      481 ggtgctggtc agctcaccat tgcgcaaaac ggcagctacg tgctgtcggg ggcgcagtcg
      541 atggcgctaa ccggcgatat agtcgttgat gatggtgcgg tgctttcgct ggaaggcgac
      601 gcggcagatc tcgccgctct ccaggacgat ccgcagtcga tcgtgttaaa cggcggtgtg
      661 ctcgatctct ctgatttctc cacctggcag agcggcacat catacaatga tggccttgaa
      721 gtcagtggca gcagcggaac ggttatcggc agtcaggatg tggtagatct tgcaggtggc
      781 gacaatttgc atatccgcgg cgacgggaaa gatggcgtct acgtggtggt cgatgcgagc
      841 gacgggcagg taagtctggc aaacaataat agttatttgg gcacaacaca aatcgcctcc
      901 ggtacgctga tggtgagcga caactcgcag cttggagata cccactataa ccgccaggtt
      961 atctttaccg ataagcaaca agaaagcgtg atggagatta ctgccaatgt cgatactcgc
     1021 tctacgacga ctgagcatgg gcgtgatatt gaaatgcgcg ccgacggtga agtggcagtt
     1081 gatgcggggg tagacacgca gtggggcgca ctgatggctg acagcagcgg gcagcatcag
     1141 gatgagggta gcacatttac taaaacgggg gcgggtacgc tggagctgac cgccagcggt
     1201 acaacgcagt cggcagtacg tgtcgaagaa ggcaccctga aaggcgatgt tgcggatatc
     1261 tttccttatg cttcgtcact gtgggtcggt gatggggcga cgttcgttac tggcgcggat
     1321 caggatattc agtctatcga tgctacttcc agcggcacta tcgatatcag cgatggtacg
     1381 gttttgcgtc tgaccgggca ggatacttcc gtcgccctta atgcctcatt gtttaacggc
     1441 gatgggacgc tggtgaatgc taccgatggc gtgacgttga caggtgagct taataccaac
     1501 cttgaaactg acagcctgac ctatcttgcc gacgtgacgg ttaatggtga tctgaccaat
     1561 acgtccggtg cggttagcct gcaaaatggc gtcgctggcg atacgctaac ggtaaacggt
     1621 gattatactg gcggcggtac gctactgttc gacagcgaat taaacggcga tgactcggta
     1681 agcgatcaat tggtgatgaa cggtaatact gctggcaaca caactgtggt ggttaactcc
     1741 attacaggga ttggtgagcc gacatcgaca ggcattaaag tggttgattt cgcagctgat
     1801 cccacgcagt ttcaaaacaa tgcacagttc agtctggctg gcagtggcta cgtcaacatg
     1861 ggagcgtatg actacacgct agtggaagat aacaacgact ggtatctgcg atcgcaagaa
     1921 gtaacgccgc catcgccacc tgatccagac ccggacccaa cacccgatcc tgacccaaca
     1981 cccgatccaa tccccgctta ccagccggtg ctgaatgcca aagttggcgg ttatctcaat
     2041 aacctgcggg cggcaaatca ggcgtttatg atggagcgac gcgatcacgc tggtggcgat
     2101 ggtcagacgc tgaatttacg tgttatcggc ggacgttatc attacacggc ggcagggcaa
     2161 ctggctcagc aggaagacac ttctacggtg cagcttagcg gcggcttgtt tagcgggcgc
     2221 tggggcacgg atggcgagtg gatgcttggg gcggttggtg gctacagtga taatcagggc
     2281 gacagccgct cgaatatgac cggaactcgc gccgataacc agaaccacgg ttatgccgtt
     2341 gggctgacct caagctggta tcagcacggt aattagaagc agggggcctg gctggatagc
     2401 tggctgcaat acgcgtggtt taacaatgat gtttccgaac atgaagatgg cgtggatcgt
     2461 taccattcgt cggggattat cgcctcgctg gaagccgggt atcagtggtt accggggcgt
     2521 ggtgtggtga ttgaaccgca ggcgcaggtg atttatcagg acgtgcagca ggatgatttt
     2581 accgccgcta accatgcgcg cgtgtcacaa tcgcaggggg atgatattca gacgcggctg
     2641 ggtttacaca gcgaatggcg taccgcggtt ggtgtcacac caacattaga tctgaattat
     2701 tatcacgatc cccatgcgac ggaaattgaa gaagatggca gcactatcag tgacgatgcg
     2761 gtgaagcgac ggggtgaaat aaaagtggga gtcacgggca atatcagtca gcgagtgtcc
     2821 ctgcgcggca gcgtggcgtg gcagaaaggg agtgatgatt ttgcccagac ggcagggttt
     2881 ttgtcgatga cggtgaaatg gtaatcactg tctagtcggc acggttgccg gatgcggcgt
     2941 aaacgcctta tccggcctac ggggagcatt tgtaggcctg ataagacgcg gcaagcgtcg
     3001 catcaggcat tggctcggcc aaacaacaac ctcacttatt ctgcgtgtgc agcatatagt
     3061 tcacatccac gccggggccg agtttaaaag tattagtgag cgggttgtaa tgtagcccag
     3121 ttatatgtcg ctctttaagg cttgtctgat ctacccagcc aagcagttct gccggtttaa
     3181 taaacttctt cacatcatgc gtgcctttgg gcaccatgcg caaaatatat tccgcgccaa
     3241 ccaccgccat cagccatgac ttgccgttgc ggttaagcgt cgagaaaaag acatcgccgc
     3301 ctggtttcac cagttgcgca caggctctga ccactgactg cggatcgggg acgtgctcca
     3361 gcatctccat gcaggtcacc acatcatact gcccggcatg ttttgccgcg tgctcttcca
     3421 cggtttcctg cacataatcc acctgaatgc cgctttccag cgcgtgcagt tttgccacct
     3481 gcaatggctc aaagcccata tccagaccgg tcaccgtcgc gccttcgcgc gccatactct
     3541 cggccagaat gccgccgcca caaccgacat cgagcacctt tttgccaaat aaaccgccag
     3601 cacgctcggc aatatagccc agacgcagcg ggttaatgcg gtgcaacggt ttgaactcac
     3661 cttcaagatc ccaccagcgg gaggcgacgg cttcaaattt agcgatctct tcgtggtcta
     3721 cgttatggtt ttccggcgat ttttcggcat tcattggcac ttctactccg taattggcaa
     3781 gacaaacgag tatatcaggc attggatgtg aataaagcgt ataggtttac ctcaaactgc
     3841 gcggctgtgt tataatttgc gacctttgaa tccgggatac agtagaggga tagcggttag
     3901 atgagcgacc ttgcgagaga aattacaccg gtcaacattg aggaagagct gaagagctcc
     3961 tatctggatt atgcgatgtc ggtcattgtt ggccgtgcgc tgccagatgt ccgagatggc
     4021 ctgaagccgg tacaccgtcg cgtactttac gccatgaacg tactaggcaa tgactggaac
     4081 aaagcctata aaaaatctgc ccgtgtcgtt ggtgacgtaa tcggtaaata ccatccccat
     4141 ggtgacgcgg cggtttatga cacgatcgtc cgtatggcgc agccattctc gctgcgttac
     4201 atgctggtag acggtcaggg taacttcggt tccatcgacg gcgactctgc ggcggcaatg
     4261 cgttatacgg aaatccgtct ggcgaaaatt gcccatgaac tgatggccga tctcgaaaaa
     4321 gagacggtcg atttcgttga taactatgac ggcacggaaa aaattcccga cgtcatgcca
     4381 accaaaattc ctaacctgct ggtgaacggt tcttccggta tcgccgtagg tatggcaacc
     4441 aacatcccgc cgcacaacct gacggaagtc atcaacggtt gtctggcgta tatcgatgat
     4501 gaagacatca gcattgaagg gctgatggaa cacatcccgg ggccggactt cccgacggcg
     4561 gcaatcatta acggtcgtcg cggtattgaa gaagcttacc gtaccggtcg cggcaaggtg
     4621 tatatccgcg ctcgcgcaga agtggaagtt gacgccaaaa ccggtcgtga aaccattatc
     4681 gtccacgaaa ttccgtatca ggtaaacaaa gcgcgcctga tcgagaagat tgcggaactg
     4741 gtaaaagaaa aacgcgtgga aggcatcagc gcgctgcgtg acgagtctga caaagacggt
     4801 atgcgcatcg tgattgaagt gaaacgcgat gcggtcggtg aagttgtgct caacaacctc
     4861 tactcccaga cccagttgca ggtttctttc ggtatcaaca tggtggcatt gcaccatggt
     4921 cagccgaaga tcatgaacct gaaagacatc atcgcggcgt ttgttcgtca ccgccgtgaa
     4981 gtggtgaccc gtcgtactat tttcgaactg cgtaaagctc gcgatcgtgc tcatatcctt
     5041 gaagcattag ccgtggcgct ggcgaacatc gacccgatca tcgaactgat ccgtcatgcg
     5101 ccgacgcctg cagaagcgaa aactgcgctg gttgctaatc cgtggcagct gggcaacgtt
     5161 gccgcgatgc tcgaacgtgc tggcgacgat gctgcgcgtc cggaatggct ggagccagag
     5221 ttcggcgtgc gtgatggtct gtactacctg accgaacagc aagctcaggc gattctggat
     5281 ctgcgtttgc agaaactgac cggccttgag cacgaaaaac tgctcgacga atacaaagag
     5341 ctgctggatc agatcgcgga actgttgcgt attcttggta gcgccgatcg tttgatggaa
     5401 gtgatccgtg aagagctgga gctggttcgt gaacagttcg gtgacaaacg tcgtactgaa
     5461 atcaccgcca acagcgcaga catcaacctg gaagatctga tcactcagga agatgtggta
     5521 gtgacgctct ctcaccaggg ctacgttaag tatcagccgc tttctgaata cgaagcgcag
     5581 cgtcgtggcg ggaaaggtaa atctgccgca cgtattaaag aagaagactt tatcgaccga
     5641 ctgctggtgg cgaacactca cgaccatatt ctgtgcttct ccagccgtgg tcgcgtctat
     5701 tcgatgaaag tttatcagtt gccggaagcc actcgtggcg cgcgcggtcg tccgatcgtc
     5761 aacctgctgc cgctggagca ggacgaacgt atcactgcga tcctgccagt gaccgagttt
     5821 gaagaaggcg tgaaagtctt catggcgacc gctaacggta ccgtgaagaa aactgtcctc
     5881 accgagttca accgtctgcg taccgccggt aaagtggcga tcaaactggt tgacggcgat
     5941 gagctgatcg gcgttgacct gaccagcggc gaagacgaag taatgctgtt ctccgctgaa
     6001 ggtaaagtgg tgcgctttaa agagtcttct gtccgtgcga tgggctgcaa caccaccggt
     6061 gttcgcggta ttcgcttagg tgaaggcgat aaagtcgtct ctctgatcgt gcctcgtggc
     6121 gatggcgcaa tcctcaccgc aacacaaaac ggttacggta aacgtaccgc agtggcggaa
     6181 tacccaacca agtcgcgtgc gacgaaaggg gttatctcca tcaaggttac cgaacgtaac
     6241 ggtttagttg ttggcgcggt acaggtagat gactgcgacc agatcatgat gatcaccgat
     6301 gccggtacgc tggtacgtac tcgcgtttca gaaatcagca tcgtgggccg taacacccag
     6361 ggcgtgatcc tcatccgtac tgcggaagat gaaaacgtag tgggtctgca acgtgttgct
     6421 gaaccggttg acgaggaaga tctcgatacc atcgacggca gtgccgcgga aggggacgat
     6481 gaaatcgctc cggaagtgga cgttgacgac gagccagaag aagaataatt ttacttcttc
     6541 atgccaaaag ggagctatct cccttgtttg aattgaaaag tccaggctgc aaagtctggg
     6601 cttttgtcgt attagggcac ggtaaagttt ggctgtgccc gtaaaaaatg gctggctata
     6661 cacaaggaat gtggcaatga gtggtgaaaa aaaggcgaaa ggctggcggt tctatggtct
     6721 tgtaggtttt ggcgcaatag tactgctttc cgctggcgtc tgggcgttgc aatatgctgg
     6781 cagtgggcca gaaaaaacgt tgtcgccgct ggtgatgcac aacaatctgc aaatcgatct
     6841 caatgagccg gacctctttc tcgacagcga ctctctgagc cagcttccca aagatctcct
     6901 caccattccg tttctccacg atgttctgag tgaagatttc gttttctatt atcagaatca
     6961 tgccgatcgt ctgggcattg aaggcagcat tcgtcgcatt gtctatgaac acgatctcac
     7021 gctgaaagat aaactctttt cgtcactctt agatca
//
LOCUS       DAJTMC010000192         7026 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.192, whole genome shotgun sequence.
ACCESSION   DAJTMC010000192 DAJTMC010000000
VERSION     DAJTMC010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7026
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.192"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            <1..247
                     /locus_tag="OO130_003693"
     CDS             <1..247
                     /locus_tag="OO130_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5366936.1"
                     /translation="HARLFGFTAEDIMDFWQHKAPQKYSAFELAFEFGHRVIAELILN
                     TLNKMAESFGFTDNPRYIAEKNYMEALLKKASPHTVR"
     gene            complement(216..1952)
                     /gene="aceK"
                     /locus_tag="OO130_003694"
     CDS             complement(216..1952)
                     /gene="aceK"
                     /locus_tag="OO130_003694"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HCR5366937.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     LAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAAFLLRVKEHYTRLLPDYPRFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
                     PLRLRWQNKSRDIHYIIRHLTETLGTDNLAESHLQVANELFYRNKAAWLVGKLITPSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDKFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPALMEL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASELWYSV
                     SPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVYAYRRR
                     QRFSVRYGEMLF"
     gene            complement(2233..3530)
                     /gene="aceA"
                     /locus_tag="OO130_003695"
                     /pseudo
     CDS             complement(2233..3530)
                     /gene="aceA"
                     /locus_tag="OO130_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
     gene            complement(3560..5161)
                     /gene="aceB"
                     /locus_tag="OO130_003696"
     CDS             complement(3560..5161)
                     /gene="aceB"
                     /locus_tag="OO130_003696"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HCR5366938.1"
                     /translation="MTEQATTTDELAFTRPYGEQEKQILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISETASICDADWKIRGIPADLEDRRVEITGPVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEEHNNQVLNKVKADKSLEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     MREQDAPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKMLSNGKPVTKALFRQMLGEEMKVIASELGEERFSQGRFGDAA
                     RLMEQITTSDELIDFLTLPGYCLLA"
     gene            complement(5430..6359)
                     /gene="metA"
                     /locus_tag="OO130_003697"
     CDS             complement(5430..6359)
                     /gene="metA"
                     /locus_tag="OO130_003697"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HCR5366939.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLDPEVPYNYFPHNDPQNTPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            6516..6963
                     /locus_tag="OO130_003698"
                     /pseudo
     CDS             6516..6963
                     /locus_tag="OO130_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
BASE COUNT         1684 a         1807 c         1826 g         1709 t
ORIGIN      
        1 ccatgcacgc ctgtttggat ttaccgctga agatattatg gatttttggc aacacaaagc
       61 gccacaaaaa tactctgcct ttgagttggc ttttgaattt ggtcaccggg ttattgctga
      121 attaatcctt aatacattaa ataagatggc tgaaagcttt ggctttacgg ataaccctcg
      181 atacattgcg gagaaaaatt atatggaagc tttactcaaa aaagcatctc cccataccgt
      241 acgctaaatc tttgcctgcg ccgatacgca taaacatctt ccacatgccc ttcacgtatg
      301 cggttttgta gtgcgcgcca gtaatcagcg cggaacaggt cggcgtgcat ctcttcaaac
      361 agcggaccaa tacgcgggtc ggcgcatagc cagtggcgaa actcttccgg gaaaacatcg
      421 cccggcgaga cgctgtacca cagttcgctg gcaagttcgt cttccggata gcgcggcggc
      481 gggatgtcgc ggaaattcac ttccgtcatg tagcaaattt catcgtaatc ataaaaaacc
      541 acacgcccgt gacgggtgac accgaagttt ttaaacagca tgtcgccagg aaaaatgtta
      601 gcagcggcaa gctggcgaat agcgttaccg tattcttcaa tggcgtcgcg caactgctga
      661 ccttccactt gttccagcca gatattgagc ggcaccatcc gccgctcgat atagagatgg
      721 cgaattacaa tctgctcacc aagatcggtg attttttccg ctgcttcctg gagcagtaat
      781 tccattaatg ccggggaaat atgccgcttc tccagcacaa agttttcaaa ctcctgggtg
      841 tccgccattc ggcccacacg gtcgtgctct ttcaccagtt gatagcaggc acgaacgtga
      901 gcggcagaca tctctttctg cggcgcgaac ttgtctttga tgactttgaa tacccgatca
      961 aagcccggca gcgtaaacac caacatcacc attccacgaa tacccggcgc ttcaatgaac
     1021 tgctcattac agccctgtag ataaacgaga tattcgcggt agctttcggt tttggcgtgc
     1081 ttctggcagc cgatagccat atacaattca gcagtggttt tacctggcag aatttcccgt
     1141 agccactcga ccagcgctgc gggcagcggt gcataaacca taaaataaga acgcgcaaag
     1201 ccaaaaacaa tgctcgcttc ggcggtcgtc gtcaggcagg tatcaataaa taactcgccg
     1261 tcgtccgtct ggtggatcgg cagtaagaag ggcaacgtgc cggaaggcgt gatcagtttg
     1321 cccaccagcc aggcggcttt attgcggtaa aacagttcgt tcgccacctg taaatgactt
     1381 tccgcgaggt tgtctgtccc cagcgtttcc gtcagatggc gaatgatgta atgaatgtca
     1441 cggcttttat tctgccagcg caggcgcagc ggcaggtcgc tgataacgcg catcagtaga
     1501 gattcccagc cgtgatcggg gtgaaagtct ttcgccagcg ggcggggaat ggtacgaaag
     1561 cggcgctctg gctgagagct aaagataaaa agccgctcgg gagtaagcga gcggtggtca
     1621 aataaccgac agtacacgga gttaaaaaag ctctccgcaa tctcgaagcg cgggtaatcc
     1681 ggcaacagcc gcgtgtaatg ctctttaacg cgcagtaaaa atgccgcgtc cgtactttgg
     1741 ccgttagtaa tgcagcgcag ttgctccacg accagaccaa cgtgatgatc gtaaagatgg
     1801 atacggtttt tcatcgccag ctggacagca tgccagtcgg cctgttcgaa acgctgctgc
     1861 gccccggaag tcacttcgag gaatcgacca tactgagcgt cgaagccttg caaaatggtt
     1921 tgagcaatca gtaattccag gccacgcggc atttagcgcc ctcatcagga gcagagaatt
     1981 gccggatgcg gcgtaaacgc cttatccagc ctacgttcgg caacggttgt aggcatgata
     2041 agacgcgcaa gcgtcgcatc aggcatcgtg caccgattgc cggatgcggc gtaaatgcct
     2101 tatccggcct acgttcggca acggctgtag gcatgataag acgcgcaagc gtcgcatcag
     2161 gcatcgtgca ccgattgccg gatgcggcgt gaacgcctta tccggcctac agtcagcaac
     2221 ggttgttgtt gcttagaact gcgattcttc agtggagccg gtcagcgcgg tgactgaaga
     2281 cgtgccgccc tgaataatag tcgtcacttt atcgaagtaa cctgtaccca cttcctgctg
     2341 gtgagatacg aaggtatagc catctttcgc ggcggcaaat tccggctgct gcactttctc
     2401 aacgtagtgc ttcataccct cgccctggtc ataggcgttt gccaggtcaa acatgttgaa
     2461 ccacatgctg tggatacctg ccagggtgat gaactggaac ttgtagccca tatccgacag
     2521 ctgctgctgg aagctggcaa tagttttgtc gtcgaggttt ttctgccagt tgaacgacgg
     2581 cgagcagtta taagccagca gtttgcccgg atatttcgcg tggatagctt gtgcaaagcg
     2641 acgcgccagt tccagatccg gcgtggaggt ttcacaccag accaggtcag catatggcgc
     2701 atacgccagg ccacggctga tcgcttgctc aatgcccgca tgagtacgga agaagccttc
     2761 actggtacgc tcgccggtaa taaattcgct gtcatacggg tcgcaatcgg aggtgatcag
     2821 atccgcggca tcggtacgag caaccagtag ggttggaacg cccgtcacgt cagctgccag
     2881 acgcgccgcg accagtttct gaatagcttc ttgagttggc actaaaactt tgccgcccat
     2941 gtgaccgcat ttcttcactg acgccagctg atcttcgaag tgaactgccg ctgcaccggc
     3001 ttcaatcatc gctttcatca gttcaaaggc attcaggaca ccgccaaaac cggcttccgc
     3061 atcggcaacg atcggcagga agtaatcgac atagcgcgga tcgcccggct caatgcccgc
     3121 ggaccattgg atctgatccg cacggcggaa agtattgttg atccgctcca ccacagctgg
     3181 caccgagttt gccggataga gcgactgatc cggatacatg ctggccgcca ggttagcgtc
     3241 cgccgctacc tgccatcccg acagatagac tgcttcaata cccgctttcg cctgttgcag
     3301 cgcctgaccg ccagtcagtg caccgaggct gttgatgtag cttttttcga ctcgccatgc
     3361 agcagacgcc acattctcgc cgcgcccagt tgcgccaggg tgcattcagg attgactgaa
     3421 tcgcgtaatt tcaccacatc ttccgcgctg tatgggcgag taatgccttc ccaacgcggt
     3481 tgagtccact ctttctgtaa ttcttcaatt tgttgtgtac gggttttcat gtgcagatgc
     3541 tccatagtta tgtggtggtt tacgctaaca ggcagtagcc tggcagggtc aggaaatcaa
     3601 ttaactcatc ggaagtggtg atctgttcca tcaagcgtgc ggcatcgcca aaacgcccct
     3661 gggagaaacg ttcttcgccc agttcgctgg caatgacttt catctcttcg cccagcatct
     3721 ggcggaacaa ggctttagtt accggtttgc cattgctcaa cattttttga tgatggatcc
     3781 actgccagat tgaggtacgg gaaatttcag ccgtcgccgc atcttccatc aggccataaa
     3841 tcggcacaca gccgttgcca gagatccacg cttcgatgta ctgcacggct acgcgaatat
     3901 ttgcgcgcat accttcttcg gtgcgttcac cgtcacaagg tgccagtagc tgatcggcag
     3961 taatcggcgc gtcttgttcg cgcatcactt caagctgatt tttacgggag ccgagaatgt
     4021 cgttgaatac cgccattgcc gtatccgcaa ggcctgggtg agcgatccat gtgccatcgt
     4081 gaccgttatt ggcttccagc gatttatccg cttttacttt gttgagcacc tggttattgt
     4141 gctcttcatc tttgctcgga ataaacgccg ccatgccgcc catcgcaaaa gcaccgcgtt
     4201 tatggcaggt tttaatcaac aggcgtgagt aagcattcag gaatggttta tccatcgtca
     4261 ctgcctgtct gtctggcagg acgcgatcgg gatagttttt caacgttttg atatagctga
     4321 agatgtaatc ccaacgaccg cagttcagac caacaatatg gtcacgcagc gcgtgaagga
     4381 tttcatccat ctggaacacg gcgggcagcg tttcaatcag caacgtcgcc ttgatggtgc
     4441 cgcgcggcag attaaagcga tcttctgcat agctgaagac ttcgctccac caggccgctt
     4501 cctgccagga ctgggttttc ggcagataga aatagggacc actgcccttt gccaacagag
     4561 cctgatagtt gtggaagaaa tagagcgcaa aatcaaacag gctgccgggg attgcctcac
     4621 cacgccaggt gacatgtttt tccggcaagt gcagaccgcg tacccgacaa atcaaaaccg
     4681 ctggattggg cttgagctgg taaattttgc ctgcttcatt ggtatagctg atggtaccgt
     4741 taactgcatc acgcaggtta atttgcccgt cgatcacttt gttccagtcc ggggccagtg
     4801 aatcttcgaa atcggccata aagactttca cattggcgtt gagcgcgttg atcaccatct
     4861 tgcgctctac cgggccagtt atctctacgc ggcggtcttc taagtccgca ggaatcccgc
     4921 gaattttcca atcagcatcg caaatggaag ctgtttccga aataaaatca ggcaacgttc
     4981 cgttatcaat atcttgctgc tgctgaatgc gcgctgccag aagtttattt cgttgtggcg
     5041 taaaatgcgt caccagctca gtcagaaatt ctaccgcttc ggcagtaaga atttgcttct
     5101 cctgctcgcc atacggcctt gtgaaagcca gttcatcggt tgttgttgcc tgttcagtca
     5161 tcgtgcagct cctcgtcatg gatccgaaaa cttccccact gtgaacgaag gatcgttgtg
     5221 cgcttttcgt tcagcacaac taagactact catttaaaat gcaaaatcaa aaacaatttc
     5281 cattttaatt gaaaatgctt gataacttat tgataacaat gtgaataaaa tttattcacc
     5341 tcctgaggtg cgtttcggaa ataaaaaagg cacccgaagg tgcctgaggt aaggtgctga
     5401 atcgcttaac gatcgactat cacagaagat taatccagcg ttggattcat gtgccgtaga
     5461 tcgtatggcg tgatctgata gacgtaatag ttgagccagt tggtaaacag caaattaccg
     5521 tgactacgcc agctcgctcg cggtgtattt tgcggatcat tgtgcgggaa atagttatac
     5581 ggtacttccg ggtctagtcc ggcttccaca tcgcggaaat attcctgcgc cagcgtttgc
     5641 gcatcatatt cgggatggcc cgtcacaaag gcaatgcgct tatctttgct ggcaaacaga
     5701 tatgcatccc cttcttccgt ctctgccaga atttccagat cggtgtaatc acgaatcaac
     5761 gctgccggaa agtcagcata gcgcgaatgt ggtgccagga atgaatcatc aaagccacgc
     5821 gtcagaagcg catgaggatg gagaatatga tgctcgtaaa cgccagagag tttgtcggtg
     5881 cgagtttgct taggaatgcc gtagaggata ttgagcgcgg cctgtaccgc ccagcagaca
     5941 aacagcgtcg aggtgacgtg atctttcgac cactccagca cctgtttgat ctgcggccag
     6001 taagcgacat cattaaactc caccaggccc agcggcgcac cggttacaat cagaccgtca
     6061 aagttctgtt cctgaatatc ttcaaagtta cagtagaagt tgttcagatg ctctgcgggc
     6121 gtgttgcgcg attcacggga atcgatgcgc aacagctgaa tatcgacctg caaaggtgag
     6181 tttgaaagca ggcgcagaaa ctgattttca gtttcaatct tcttcggcat caggttaagg
     6241 atcagaacct taagtggacg aatttcctga ccagacgcac gagaagttgt catcacaaag
     6301 acgttttctt cacgcaagaa attgacggcg ggtagctcgt ccggcacacg aatcggcata
     6361 acctgattac ctcactacat acacttatac gtttagacat ccagatagct gaagataacc
     6421 agaaaatttg ccaatgtcga gcctgcatgt tgaaggtgag aaagtttcaa ggaaaagact
     6481 acgttagaat ataagaatga ctaaaaggag agaaaatggt tattagtatt cgccgctcac
     6541 ggcatgagga aggggaggaa ctcgttgcga tttggtgtcg atctgtcgat gccacacacg
     6601 attttttatc agcagagtat cgggccgagc tggaggtgct agctagttcg ttccttcctg
     6661 ccggaagcgc cgttgtggat cgcggttaat gaacgggatc agccggttgg gtttatgttg
     6721 ttaagtgggc agcatatgga tgcgctgttt atcgatcctg atgtgtgcgg ctgcggcgtg
     6781 ggtcggatgc tggtggagca tgcgctctcg atggcaccgg aactgacaac caacgttaat
     6841 gagcaaaatg agcaggcggt tgggttctat aagaaggtgg gttttaaggt tacgggacgc
     6901 tctgaggtgg acgatttggg gaaaccgtat ccgttgctga atctggcgta cgtgggggaa
     6961 taagccgccc ctgccgtgcc tgcccaggct ggagaagacg cgccagcgtc gcacccggca
     7021 tttttt
//
LOCUS       DAJTMC010000193         6801 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.193, whole genome shotgun sequence.
ACCESSION   DAJTMC010000193 DAJTMC010000000
VERSION     DAJTMC010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6801
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.193"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            187..2103
                     /gene="dnaK"
                     /locus_tag="OO130_003699"
     CDS             187..2103
                     /gene="dnaK"
                     /locus_tag="OO130_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HCR5366940.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            2192..3322
                     /gene="dnaJ"
                     /locus_tag="OO130_003700"
     CDS             2192..3322
                     /gene="dnaJ"
                     /locus_tag="OO130_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HCR5366941.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGQHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(3426..3578)
                     /gene="mokC"
                     /locus_tag="OO130_003701"
     CDS             complement(3426..3578)
                     /gene="mokC"
                     /locus_tag="OO130_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HCR5366942.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            4164..5330
                     /gene="nhaA"
                     /locus_tag="OO130_003702"
     CDS             4164..5330
                     /gene="nhaA"
                     /locus_tag="OO130_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HCR5366943.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMMMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLCPSV"
     gene            5396..6295
                     /gene="nhaR"
                     /locus_tag="OO130_003703"
     CDS             5396..6295
                     /gene="nhaR"
                     /locus_tag="OO130_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HCR5366944.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAAR"
     gene            complement(6333..>6801)
                     /locus_tag="OO130_003704"
     CDS             complement(6333..>6801)
                     /locus_tag="OO130_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HCR5366945.1"
                     /translation="RFNYNLNTILLVNLMTCGFEDQSIDTGRFVLSRINNENYQFHHI
                     RFDCIQGEQGELVFEPSDVEYYFEPVTIQESGTSILKNELENSEDGAGQVGFQLSNDG
                     TNEIQYGKSNYYSFQHPHEGSNQIPLFIRPRTYGNNVSSGQIMSRVKIVVMYN"
BASE COUNT         1738 a         1600 c         1757 g         1706 t
ORIGIN      
        1 tctgcgcaaa agcacaaaaa atttttgcat ctcccccttg atgacgtggg ttacgacccc
       61 atttagtagt caaccgcagt gagtgagtct gcaaaaaaat gaaattgggc agttgaaacc
      121 agacatttcg cccctattac agactcacaa ccacatgatg accgaatata tagtggagac
      181 gtttagatgg gtaaaataat tggtatcgac ctgggtacta ccaactcttg tgtagcgatt
      241 atggatggca ccactcctcg cgtgctggag aacgccgaag gcgatcgcac cacgccttct
      301 attattgcct atacccagga tggtgaaact ctggttggtc agccggctaa acgtcaggca
      361 gtgacgaacc cgcaaaacac cctgtttgcg attaaacgcc tgattggccg ccgcttccag
      421 gacgaagaag tacagcgtga tgtttccatc atgccgttca aaattattgc tgctgataac
      481 ggcgacgcat gggtcgaagt taaaggccag aaaatggcac cgccgcagat ttctgctgaa
      541 gtgctgaaaa aaatgaagaa aaccgctgaa gattacttgg gtgaaccggt aactgaagct
      601 gttatcaccg taccggcata ctttaacgat gctcagcgtc aggcaaccaa agacgcaggc
      661 cgtatcgctg gtctggaagt aaaacgtatc atcaacgaac cgaccgcagc tgcgctggct
      721 tacggtctgg acaaaggtac tggcaaccgt actatcgcgg tttatgacct ggggggtggt
      781 actttcgata tttctattat cgaaatcgac gaagttgatg gcgaaaaaac cttcgaagtt
      841 ctggctacca acggtgatac ccacctgggt ggtgaagact tcgacagccg tctgatcaac
      901 tacctggttg aagaattcaa gaaagatcag ggcattgacc tgcgcaacga tccgctggca
      961 atgcagcgcc tgaaagaagc ggcagaaaaa gcgaaaatcg aactgtcttc cgctcagcag
     1021 accgacgtta acctgccgta catcactgca gatgcgaccg gtccgaaaca catgaacatc
     1081 aaagtgactc gtgcgaaact ggaaagcttg gttgaagatc tggtaaaccg ttccattgag
     1141 ccgctgaaag ttgcactgca ggacgctggc ctgtccgtat ctgatatcga cgacgttatc
     1201 ctcgttggtg gtcagactcg tatgccaatg gttcagaaga aagttgctga gttctttggt
     1261 aaagagccgc gtaaagacgt taacccggac gaagctgtag caatcggtgc tgctgttcag
     1321 ggtggtgttc tgactggtga cgtgaaagac gtactgctgc tggacgttac cccgctgtct
     1381 ctgggtatcg aaaccatggg cggtgtgatg acgacgctga tcgcgaaaaa caccactatc
     1441 ccgaccaagc acagccaggt gttctctacc gctgaagaca accagtctgc ggtaaccatc
     1501 catgtgctgc agggtgaacg taaacgtgcg gctgataaca aatctctggg tcagttcaac
     1561 ctggatggta tcaacccggc accgcgcggc atgccgcaga tcgaagttac cttcgatatc
     1621 gatgctgacg gtatcctgca cgtttccgcg aaagataaaa acagcggtaa agagcagaag
     1681 atcaccatca aggcttcttc tggtctgaac gaagatgaaa tccagaaaat ggtacgcgac
     1741 gcagaagcta acgccgaagc tgaccgtaag tttgaagagc tggtacagac tcgcaaccag
     1801 ggcgaccatc tgctgcacag cacccgtaag caggttgaag aagcaggcga caaactgccg
     1861 gctgacgaca aaactgctat cgagtctgca ctgactgcac tggaaactgc tctgaaaggt
     1921 gaagacaaag ccgctatcga agcgaaaatg caggagctgg cacaggtttc ccagaaactg
     1981 atggaaatcg cccagcagca acatgcccag cagcagactg ccggtgctga tgcttctgca
     2041 aacaacgcga aagatgacga tgttgtcgac gctgaatttg aagaagtcaa agacaaaaaa
     2101 taatcgccct ataaacgggt aattatactg acacgggcga aggggaattt cctctccgcc
     2161 cgtgcattca tctaggggca atttaaaaaa gatggctaag caagattatt acgagatttt
     2221 aggcgtttcc aaaacagcgg aagagcgtga aatcaaaaag gcctacaaac gcctggccat
     2281 gaaataccac ccggaccgta accagggtga caaagaggcc gaggcgaaat ttaaagagat
     2341 caaggaagct tatgaagttc tgaccgactc gcaaaaacgt gcggcatacg atcagtatgg
     2401 tcatgctgcg tttgagcaag gcggcatggg cggcggcggt tttggcggcg gcgcagactt
     2461 cagcgatatt tttggtgacg ttttcggcga tatttttggc ggcggacgtg gtcgtcaacg
     2521 tgcggcgcgc ggtgctgatt tacgctataa catggagctc accctcgaag aagctgtacg
     2581 tggcgtgacc aaagagatcc gcattccgac tctggaagag tgtgacgttt gccacggtag
     2641 cggtgcaaaa ccaggtacac agccgcagac ctgtccgacc tgtcatggtt ctggtcaggt
     2701 gcagatgcgt cagggtttct ttgccgtgca gcagacctgt ccacactgtc agggccgcgg
     2761 tacgctgatc aaagatccgt gcaacaaatg tcatggtcat ggtcgtgttg agcgcagcaa
     2821 aacgctgtcc gttaaaatcc cggcaggggt ggacactgga gaccgcatcc gtcttgcggg
     2881 cgaaggtgaa gcgggccagc atggcgcacc ggcaggcgat ctgtacgttc aggttcaggt
     2941 taaacagcac ccgattttcg agcgtgaagg caacaacctg tattgcgaag tcccgatcaa
     3001 cttcgctatg gcggcgctgg gtggcgaaat cgaagtaccg acccttgatg gtcgcgtcaa
     3061 actgaaagtg cctggcgaaa cccagaccgg taagcttttc cgtatgcgcg gtaaaggcgt
     3121 caagtctgtc cgcggtggcg cacagggtga tttgctgtgc cgcgttgtcg tcgaaacacc
     3181 ggtaggcctg aacgagaagc agaaacagct gctgcaagag ctgcaagaaa gcttcggtgg
     3241 cccaaccggc gagcacaaca gcccgcgctc aaagagcttc tttgatggtg tgaagaagtt
     3301 ttttgacgac ctgacccgct aacctcccca aaagcctgcc cgtgggcagg cctgggtaaa
     3361 aatagggtgc gttgaagata tgcgagcacc tgtaaagtgg cggggatcac tccccgccgt
     3421 tgctcttact cggattcgta agccgtgaaa acagcaacct ccgtctggcc agttcggatg
     3481 tgaacctcac agaggtcttt tctcgttacc agcgccgcca ctacggcggt gatacagatg
     3541 acgatcaggg cgacaatcat cgccttatgc tgcttcattg ctctcttctc cttgacctta
     3601 cggtcagtaa gaggcactct acatgtgttc agcatatagg gggcctcggg ttgatggtaa
     3661 aatatcactc ggggcttttc tctatctgcc gttcagctaa tgcctgagac agacagcctc
     3721 aagcacccgc cgctattata tcgctctctt taacccattt tgttttatcg attctaatcc
     3781 tgaagacgcc tcgcattttt gtggcgtaat tttttaatga tttaattatt taactttaat
     3841 ttatctcttt atcgcaatta ttgacgacaa gctggattat ttttgaaata ttgtcctaac
     3901 aaataacgcc gactgacaag aaattaatta ttacttttcc taattaatcc ctcagaaatc
     3961 ctcaccttaa gctatgatta tctgagctta gggtcactcg tcagcgctta cagccgtcaa
     4021 aaactcatct caccgctgat ggcgaaaatt ctacaaaggc tcgtaaaaaa cgaattattc
     4081 ctacactata atctgatttt aacgatgatt cgtgcggggt aaaatagtaa aaacgattta
     4141 ttcacctgaa agagaaataa aaagtgaaac atctgcatcg attctttagc agtgatgcct
     4201 cgggaggcat tattcttatc attgccgcta tcctggcgat gatgatggcc aacagcggcg
     4261 caaccagtgg atggtatcac gactttctgg agacgccggt tcagctccgg gttggttcac
     4321 tcgaaatcaa caaaaacatg ctgttatgga taaatgacgc gctgatggcg gtatttttcc
     4381 tgttagtcgg tctggaagtt aaacgtgaac tgatgcaagg atcgctagcc agcttacgcc
     4441 aggccgcatt tccagttatc gccgctattg gtgggatgat tgtgccggca ttactctatc
     4501 tggcctttaa ctatgccgat ccgattaccc gcgaagggtg ggcgatcccg gcggctactg
     4561 acattgcctt tgcacttgga gtgctggcgc tgttgggaag tcgtgttccg ttagcactga
     4621 agatcttttt gatggctctg gctattatcg acgatcttgg ggccatcatt atcatcgcat
     4681 tgttctacac taatgactta tcgatggcct ctcttggcgt cgcggctgta gcaattgcgg
     4741 tactcgcggt attgaatctg tgtggtgtac gccgcacggg cgtctatatt ctggttggcg
     4801 tagtgttgtg gactgcggtg ttgaaatcgg gggttcacgc aaccttggcg ggggtaattg
     4861 tcggcttctt tattcctttg aaagagaagc atgggcgttc tccagcgaag cgactggagc
     4921 atgtgttaca cccgtgggtg gcgtatctga ttttgccgct gtttgcattt gctaatgctg
     4981 gcgtttcact gcaaggcgtc acgctggatg gcttgacctc cattctgcca ttggggatca
     5041 tcgctggctt gctgattggc aaaccgctgg ggattagtct gttctgctgg ttggcgctgc
     5101 gtttgaaact ggcgcatctg cctgagggaa cgacttatca gcaaattatg gcggtgggga
     5161 tcctgtgcgg tatcggtttt actatgtcta tctttattgc cagcctggcc tttggtagcg
     5221 tagatccaga actgattaac tgggcgaaac tcggtatcct ggtcggttct atctcttcgg
     5281 cggtaattgg atacagctgg ttacgcgttc gtttgtgtcc atcagtgtga caggacggtt
     5341 taccggggag ccataaacgg ctcccttttc attgttatca gggagagaaa tgagcatgtc
     5401 tcatatcaat tacaaccact tgtattactt ctggcatgtc tataaagaag gttccgtggt
     5461 tggcgcagcg gaggcgcttt atttaactcc acaaaccatt accggacaga tccgggctct
     5521 ggaagagcgc ctgcaaggca aattatttaa gcgcaaggga cgtggtctcg aacccagcga
     5581 gctgggagaa ctggtctatc gctatgccga taaaatgttc accttaagcc aggaaatgct
     5641 ggatattgtg aactatcgca aagaatccaa tttattgttt gacgttggcg tggctgatgc
     5701 actttccaaa cgcctggtca gtagcgtgct gaatgctgca gtggtagaag gcgagccgat
     5761 tcatcttcgc tgcttcgaat ccacccacga aatgctactg gagcaattaa gccagcataa
     5821 actggatatg atcatttccg actgtccgat agactctacg cagcaggaag gcctgttctc
     5881 cgtgagaatt ggcgaatgtg gcgtgagttt ctggtgtaca aatccaccac cagaaaaacc
     5941 gttcccggct tgtctggaag aacggcgact tttgattcct gggcgacgtt caatgttagg
     6001 gcgcaaattg cttaactggt ttaactccca gggattaaac gtagaaatcc tcggcgagtt
     6061 tgatgatgcc gcgttgatga aagcttttgg tgcaatgcac aatgcaatct tcgttgcccc
     6121 aacgctttat gcatatgact tttatgccga taaaactgtc gtagaaattg gtcgcgtcga
     6181 gaatgtgatg gaagagtacc atgctatttt tgctgagcgg atgattcagc acccggcggt
     6241 acagcgaatc tgcaatacgg attattctgc gctttttagt ccagcggcgc gttaatcggc
     6301 agctccccca aagttaaagt ggaggagata gattagttgt acataaccac gattttgact
     6361 cggctcatta tttgcccgct tgagacatta tttccatatg tacgcgggcg aataaataga
     6421 ggaatctgat tacttccttc atggggatgc tgaaaagagt agtaattgct tttaccatac
     6481 tggatttcat tcgtaccatc attggagagt tgaaagccta cttgacccgc accgtcctca
     6541 ctgttttcta actcattttt taatatgctg gttcctgact cctgtatcgt cactggttca
     6601 aagtaatatt caacatcact gggttcaaaa actaattcac cttgctcgcc ctggatgcag
     6661 tcaaatctta tatgatgaaa ctgataattt tcattattga tacgagacag tacaaatcgt
     6721 ccagtgtcga tggattgatc ttcaaaacca caggtcatta aatttaccag taaaatagta
     6781 tttaaattat aattaaagcg g
//
LOCUS       DAJTMC010000194         6798 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.194, whole genome shotgun sequence.
ACCESSION   DAJTMC010000194 DAJTMC010000000
VERSION     DAJTMC010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6798
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.194"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            174..902
                     /gene="mpaA"
                     /locus_tag="OO130_003705"
     CDS             174..902
                     /gene="mpaA"
                     /locus_tag="OO130_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HCR5366946.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPSSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(877..1842)
                     /gene="ycjG"
                     /locus_tag="OO130_003706"
                     /pseudo
     CDS             complement(877..1842)
                     /gene="ycjG"
                     /locus_tag="OO130_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
     gene            1961..2467
                     /gene="tpx"
                     /locus_tag="OO130_003707"
     CDS             1961..2467
                     /gene="tpx"
                     /locus_tag="OO130_003707"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HCR5366947.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAFGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(2511..4052)
                     /gene="tyrR"
                     /locus_tag="OO130_003708"
     CDS             complement(2511..4052)
                     /gene="tyrR"
                     /locus_tag="OO130_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HCR5366948.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGMFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(4200..5261)
                     /locus_tag="OO130_003709"
     CDS             complement(4200..5261)
                     /locus_tag="OO130_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HCR5366949.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDENQAQNFAPATLDE
                     APEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREICRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(5258..6655)
                     /locus_tag="OO130_003710"
     CDS             complement(5258..6655)
                     /locus_tag="OO130_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HCR5366950.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSVKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDTWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTIDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
BASE COUNT         1624 a         1936 c         1661 g         1577 t
ORIGIN      
        1 cgacttacat ctaagtcagc ataattctaa tatctgcaca tcaataaaaa tgcaaacaac
       61 ttccggcaaa aaaagtgata caagatcgat gacatagcgt tgaagaaaag gatttgataa
      121 tccgcaaaat ttgcacaaca ctgcctgtaa tacttcctga accagagacg atcatgaccg
      181 taacccgccc acgcgccgaa cgcggcgcat ttccgcccgg aacagaacat tacggacgtt
      241 cattattggg cgcgccgtta atctggtttc ctgcccctgc agccagccgc gaaagtggtt
      301 tgattctggc tggcactcac ggtgatgaaa actcttcggt cgtcacgctc tcctgtgctc
      361 tgcggacgtt gacaccttct ttgcgccgcc atcatgtggt gctgtgtgtg aatcctgacg
      421 gctgccagct ggggttacgg gccaatgcta atggtgtgga tttaaacaga aactttccgg
      481 cggcgaactg gaaggaaggt gaaacggttt atcgctggaa cagcgccgct gaagaacgtg
      541 atgtcgtatt actgacaggt gacaaacctg gctcagaacc tgaaacccag gcgctgtgcc
      601 agcttataca ccgcattcaa cctgcctggg tggtctcatt ccacgatcca ctggcctgta
      661 ttgaagatcc cagacacagc gaattaggcg aatggctggc ccaggcgttt gaattgcctc
      721 tggtaaccag cgtcggttat gaaacgccta gctctttcgg cagttggtgt gcagatctca
      781 acttgcattg catcaccgca gagtttccgc ccatctcctc cgacgaagcc agcgaaaaat
      841 atctctttgc gatggcaaac ttgctgcgct ggcatcctaa agatgcaatt cgcccgtcgt
      901 gaactgaagc gccggttcca catctaccgc cagccaggtc ggtccgtcaa gatcggcgaa
      961 actgacctgc ggcaccagcg gtaaagcggc gctaatggca cgagaggtac acaacatgca
     1021 gcccagcatc agactgaaac cttgtgcacg cgcttcagtc gccagcgcca gcgcttccgt
     1081 cagaccaccg gttttatcga gcttaatgtt aaccatctca tagcgccctt tcagcgcctt
     1141 cagattgcta cgtgtatgac aactctcatc agcacaaatc ggcaacggat gaataaaatt
     1201 ctccagcgcc gcatcgtcct gcgcaggaag gggttgttca agcatcgcaa cgcctaaatc
     1261 cgccaatagc tggcaacgcg ccgccaaccc ttatgcacgc caggattcat ttgcatcaac
     1321 gatcagcgtc gcatcgggca cagctgtgcg aattgccacc atccgctcac taataagatg
     1381 gttatccagc ttcactttca gtaatttcgc gcctgcctgc cagagtgttg atgcactatt
     1441 ggccatctga tcaggcgtac cgatgactac cgtctgtgca gtgataactg tctcgggaag
     1501 cgttatgccg atcaaatcag ccagcgattg ctgctgtttt cgcgcggcca gatcccacaa
     1561 tgcacaatcc agcgcattac gtgctgcgcc ggcagggaga attttttgca actcctcccg
     1621 tgtcagccct ttctctaatt gcgacacgac gctcataatt tgcgccatta ccgaggcatc
     1681 actttctcca taacgcggat acggcgtgca ttcgccggtg cctttaatac cctcttcttc
     1741 cagttcaacc accaccacgc gcgcttcact gcgacttccc cgggcaatca caaacggggt
     1801 atgtaagggc taggcttcct caaatacctt aacggttctc atctttactc cttgttgtcg
     1861 cggtggtctg cggattaagc gcaaatagcg tttgctgtgt tattgacagt tagcataaac
     1921 taggtgtgac gttaactata tgtaaacagg aaagataatc atgtcacaaa ccgttcattt
     1981 ccagggcaac ccggttacag tcgccaattc catcccgcag gcgggtagca aagcgcagac
     2041 ttttactctc gtggcaaaag atctgtctga cgtcaccctc ggtcagtttg cgggtaaacg
     2101 caaagtgctg aacattttcc cgagtattga taccggtgtt tgcgccgcat cagtacgtaa
     2161 gtttaaccaa ctggcaaccg agatcgacaa caccgttgtg ctgtgtattt ctgccgatct
     2221 gccgttcgcc cagtctcgtt tctgcggcgc agaaggtctg aacaacgtta tcaccctctc
     2281 cactttccgt aacgctgaat ttctgcaagc cttcggtgtg gcaattgctg atggcccact
     2341 gaaaggtctg gcagcgcgtg ccgttgtggt tattgacgaa aatgacaatg tgattttcag
     2401 ccagctggtg gatgaaatca ccaccgagcc ggattacgaa gcagctctgg ctgtactgaa
     2461 agcataagtt aatatgcctg atggtgttgc accatcaggc atattcgcgc ttactcttcg
     2521 ttcttcttct ggcttaagcc atattcccgc aacttattgg caatcgcggt atgtgaaacg
     2581 ccgagacgtt ttgccagttt gcgcgtgctg ggataattgc gataaagctg ggttaatacc
     2641 gagcgttcaa aacggctggt gatttcgtcc agcgaacctt ccatcgcatc ttcgcccacg
     2701 gctaccgttg cggcgtcata atccggcaac aaaatatcct gtggacgcag ctcataaccg
     2761 tccagttgtg tcagtgcgcg atatatagcg ttctttaact gccgcacatt tcccggccac
     2821 gcataacgcg taagtacagt attcaggtca gcggccagtt tcggacgcgg cacgccctgc
     2881 tcgtcggcaa agcgggcgac gaacagctca gttaacggca tgatgtcctg cggacagtca
     2941 cgtagcggcg gcagattgag cgtcaacacg ttcagacgat aatagagatc ttcacggaac
     3001 atgccttttt gcaccagttc gaccagattc ttctgcgtag cgcaaatcac ccgcacatcg
     3061 acatgcacct catggtcttc gccaacccga cggaaagtgc catcattaag aaaacgcagt
     3121 aatttcgcct gcatccgtgg tgacatttcc cctatttcat ccaacagcac cgaaccaccg
     3181 ttcgcctgct caaagaatcc tttcttccct tccggagcat gaccaaacag ttcactctcg
     3241 accgcatctt ccggtataga cgcacagttc agcgccaggt aaggtttgcc cgctctgggg
     3301 cttgcctgat ggcaggcgta ggcaaagaga tctttacctg tacctgtgtc acccgtaatc
     3361 agcagcggcg cgcttagcat cgccagtttc tgcgcctgtt cgacaacatg cttcattttc
     3421 gggctgacgg cgacaatttg actgaaggcg ctgacgtcct gggcggcgac attttgcaac
     3481 tggcggccca tacgaatcgt tgatcgcaac atcaccaccg caccggtcag gacgtgttga
     3541 tcattttcat cctgaagata aacaggcgta atctccatca ggaaattctg cccattaata
     3601 acgacatgct cgttatgcga atcttgcggt tcgctttcca gccaacgtaa aaaattaaag
     3661 ccgttaatca attgtgcggc ggtatggttg cgcaggcgat ccaatttttg cccaaaaagc
     3721 tgacagctcg ccgggttcgc catatccact ttgcttttca tatcgacaga gagcacaggt
     3781 tcgggcaacg cctccagtaa cgcgctcaac gccagatgct cacgttcgga aggcatccac
     3841 gggacagtac gcacatcggt aacacctgca atacggcgta tttcggccat cagactgctg
     3901 aaactctcaa actccagttc agcaaaattg aggtagattc gcccaatggg atcaatctca
     3961 ataccgcgta aatcaatgcc tcttagcacg agtagatcga gtaattcgcg ggtcagaccg
     4021 agtcggtctt cacaaaagac ttccagacgc atgggaacct tcacctgaaa aaagaacaat
     4081 taatatgatg atatgacact atttggatag caggaaggtt tctgtcaaca atcattgaca
     4141 caaagacgga aaaacgggca cttcggcgta aagatatccg gtcagcaccc gatattgcat
     4201 tatttttcgc tgggcgtttt gcctttttgc agcgtttctt tcacctgacc gataagctga
     4261 cgacggaaat ccccgaggcg aggtttgtca tcgtcaatcc acggcagcgg gcggcaaagc
     4321 tccatagctt taatcccgag tcgtgccgtc agaagtccag caccaattcc ctgagctgcg
     4381 cgggtagaca aacgagcagc gagatcttgc gacatccagt ccatccccac ttcgcgcacc
     4441 agttcgctgg ctccggcaaa agcgatattc agcaatacca gcttaaacag acgcaaacgg
     4501 ctgtaatacc ccagttcaat gccatacagc gtggcgatgc gattaattaa acgcagattg
     4561 cgccaggcga taaacgccat atcgaccaac gccagcgggc tgaccgcaat catcaacgtt
     4621 gattccgccg ccgaacggca gatttcgcgc cgcgcctggg catctaaaac tggctggacc
     4681 aaatgcgcat acaaactgac cacttcacgg tcgttttgcg tttcatggat tgaggcatac
     4741 cagcgttgca gcgctggatg agactgatca atacccgcct gctgcgccag tttttcgcaa
     4801 aatgcgcggc ctttgcccgt gccgtggctg tgcaacaaat cgcgcgcttc gtcgcgttca
     4861 tgggcgcgct gtcgcaagcg ccataagcgc cgccactctg ttaccacaga acctacgcca
     4921 gcgccgataa tcaatgcccc agcggcacat ccacccagcg ccacccagtc ctgagtttgc
     4981 caggcattca ttgtccactg tacaccctgc ccgacaacgc ttgcgccaaa cagagccagc
     5041 ccgcccatca ccattttgcg ccacaggctg cgtttcggac gtaacgctgc atccattacc
     5101 gcttcaactt gcccctcttc ttcaggcgct tcatcgagcg tggccggggc aaaattttgc
     5161 gcctgatttt cgtcaaaggt ctgctgcgcc ctgaattttg gattctgatc gacctccagc
     5221 ggaccgtcga aatcaatacg tggttttaac ggttcggtca tcgcaattta tctcctatta
     5281 aaaattccag cgcggcatca agacgaatat gcggcagcgg tttgtcgaca tccatcacct
     5341 gcgggcgaaa cgcttcaaac tggaaccctt gtttgtccca gaacgcctga ccgggcaaac
     5401 gcgcaggaac ttcaccggga taaacggtga gtggcgcacc atcgctgagc cggttaccgc
     5461 gcaatgccgg aattttctcg ccgttgacgt caataatgcc gctggtggtc gcctgcaccg
     5521 aggccagccc caggcaatcc atactaatcc cttcaaacgc cgcattttgc caggcatcct
     5581 gaatcagttg ttgcagtaat gaaaccatat tggcgtgctg atcgatggtc acatggtccg
     5641 ctttagtggc agcaaacaat agcttatcga taaccggcga aaacaaacgc cggaacaagg
     5701 tacgctgccc gtagtggaaa ctttgcatca gctgcgtcag tgccagacgc atatcattaa
     5761 atgcctgtgg cccactgttg agaggttgca ggcaatcaac cagcacaatc tggcggtcaa
     5821 agcgcagaaa atgattctta tagaacccct tcaccacctt ctcgcagtaa taattaaacc
     5881 gctcgcgcag cattccggca ttggtgtgct tatcggcctg cgccagtttg gactcgcccc
     5941 aggtatcgac atccggccac gggaagaatt gcagcgcggg cgcacctgcc atatctcctg
     6001 gcaagacaaa gcgccctggc tgaataaagt gcagcccctg ctgtttacag tggtggagat
     6061 aatcggtcca cgcggcggca atgtctgcca gccggttttc gtcggcaggt gctagcgggt
     6121 ccagcccttc gcacatcatt cgccatttga ccgaccattc gccgcgctga ccattgagta
     6181 agcccgtcat ctggcgtgac cagcttaaat agtcctgcgc cagcatcggc aggtcgagca
     6241 accattcgcc gggataatcc acaatctcca gatacagcgt ggaggtgtcc ttgaagtggc
     6301 gtagcagcga atcgttcgat ttaaaacgta gcgccaggcg gatttcactg acgccgcgcg
     6361 ttggcgtcgg ccaggcggga ggatcgccat ataactgcgc cagtccttca tcatatgtga
     6421 agcgcggaat gccaaagtca cgctgaggaa tgcgttttac gcccagcagg cgctcttcac
     6481 gcaccgcgct taacagcggc aagcgtgctc cggcgtgaat attgagcaac tgattgacca
     6541 tagcagtgat aaacgctgtt ttgccgctgc ggcttagtcc ggttacggcg aggcgcagat
     6601 gtctgtcgac accccgattc accagcgcat taagttcatt tttaagtcgc ttcattgctg
     6661 tcctgtgctg ctctgaagac cagaaaaaaa tcattcgtaa ggatatgaat gtgggggcca
     6721 ggccattgat tatcaactgc ccgtgttgtc atcgacgggc gaactccata agatgtgcag
     6781 cattcacgtt cggacgga
//
LOCUS       DAJTMC010000195         6790 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.195, whole genome shotgun sequence.
ACCESSION   DAJTMC010000195 DAJTMC010000000
VERSION     DAJTMC010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6790
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.195"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(85..900)
                     /gene="sul2"
                     /locus_tag="OO130_003711"
     CDS             complement(85..900)
                     /gene="sul2"
                     /locus_tag="OO130_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HCR5366951.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            2089..2238
                     /locus_tag="OO130_003712"
     CDS             2089..2238
                     /locus_tag="OO130_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5366952.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPV
                     GREAL"
     gene            complement(3005..3445)
                     /locus_tag="OO130_003713"
     CDS             complement(3005..3445)
                     /locus_tag="OO130_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HCR5366953.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
     gene            3663..4052
                     /locus_tag="OO130_003714"
     CDS             3663..4052
                     /locus_tag="OO130_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5366954.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            4096..4500
                     /locus_tag="OO130_003715"
                     /pseudo
     CDS             4096..4500
                     /locus_tag="OO130_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(4607..5443)
                     /locus_tag="OO130_003716"
     CDS             complement(4607..5443)
                     /locus_tag="OO130_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HCR5366955.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(5443..5718)
                     /locus_tag="OO130_003717"
                     /pseudo
     CDS             complement(5443..5718)
                     /locus_tag="OO130_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase APH(3')"
     gene            complement(5793..6266)
                     /locus_tag="OO130_003718"
     CDS             complement(5793..6266)
                     /locus_tag="OO130_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA14"
                     /protein_id="HCR5366956.1"
                     /translation="MKVSLIAAKAKNGVIGCGPDIPWSAKGEQLLFKALTYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSGWTSNDDNVVVFQSIEEAMDRLAEFTGHVIVSGGGEI
                     YRETLPMASTLHLSTIDIEPEGDVFFPSIPNTFEVVFEQHFTSNINYCYQIWKKG"
     gene            complement(6287..6790)
                     /locus_tag="OO130_003719"
                     /pseudo
     CDS             complement(6287..6790)
                     /locus_tag="OO130_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
BASE COUNT         1586 a         1758 c         1771 g         1675 t
ORIGIN      
        1 aaaaaaaata ttagttcgat tcaatggagg ttccttcagt tttctgatga agcgcgaata
       61 tagagaaata tcccgaatgt gcagttaacg aattcttgcg gtttctttca gcgccgccaa
      121 taccgccagc ccgtcgcgca aggggcgcgg ctcgtgtgtg cggatgaagt cagctccacc
      181 tgcggcggcg gcaagctctg cagcgagtgt cgcggccccg acatcccccg gaccacggcc
      241 tgtgagcgcg cgcagaaagg atttgcgcga aacagacaga agcaccggca aatcgaagcg
      301 cagccgcaat tcatcgaacc gcgccagcac cgagagcgag gtttcgggag cagcccccag
      361 aaaaaacccc atgccgggat caaggacaag gcggttgcgt ttgataccgg cacccgtcag
      421 cgccgcgatg cgcgcgtcaa agaacgccgc aatgtgatcc atgatgtcgc cagcgggtgc
      481 ctcgcgccga tctgcctgcc cgtcttgcac cgaatgcata acgacgagtt tggcagatga
      541 tttcgccaat tgcggataga acgcagcgtc tggaaaaccg cgaatatcat tgagataggc
      601 cacaccacgc gacaaggcat aggcttgcgt cgcgggttga taactgtcga gcgagacggg
      661 aatgccatct gccttgagcg cgtccagcac cggcgcgata cgcgcgattt ctgtgtcgga
      721 cgaaacaggc gcggcgtcgg gattgctgga tgccggaccg aggtcgatca catctgcccc
      781 ctcggccatc agcttacgcg cctgcgcaat ggctgcgtct ggcgccagat accggcctcc
      841 atcggagaaa ctgtccgagg ttatgttgac gatgccgaaa atgatgagcg atttattcat
      901 gggggcttct ataataataa taatcgagca tgagtctcat acggatgctc gggtcgaaag
      961 ggaatcccca ggcgagtaac ctgtttgcgg tgatccgttt catgatgatg tatctgtagc
     1021 attttggaaa taaatatatc atctctcaat aagtagatat atttatcttt tttgcttctt
     1081 tggtgctgca agtcaccaat acgagggaaa cctgttacgg agcggtttaa ccgtaatcaa
     1141 cagtgactgg tgtcatgttc ggaagattaa ttgatagtaa tctacaaagg atcttcttga
     1201 gatccttttc aatgaatcgt aatctcttgc tctgtaaacg aaaaaaccgc ctggggaggc
     1261 ggtttgttcg aaggttaagt cagttgggga actgcttaac ccggtaacag ggttcacgag
     1321 accgccatta ccaaaactgt tctttcagtg tagccgcagt caggcctcta cttcaagacc
     1381 tcttaatcta tcttctcgtg cactcctgtt actaatggct gctgccagtg gcgttttgtc
     1441 gtgtctttcc gggttggact caagttgata gttaccggac aaggcgcagc ggtcggactg
     1501 aacggggggt tcgtgcatac agtccagctt ggagcgaact gccttcacgg aactgagtgt
     1561 caggcgtgga atgagaaacc gcggccataa cagcggaatg acaccggtaa accgaaaggc
     1621 aggaacagga gagcgcacga gggagctacc agggggaaac gcctgggatc tttatagtcc
     1681 tgtcgggttt cgccaccact gatttgagcg tcagattttg cgatgcttgt caggggggcg
     1741 gagcctatgg aaaaacggct tcgccgtggc cttatctctt ctttgctaag tgtcttcctg
     1801 gtatcttcca ggaaatttcc gtccccgtcc gtaagccggt accgctcgcc gcagccgaac
     1861 gaccgagcgt agcgagtcag tgagcgagga agcggaatat ctcctgtatc gcatattctg
     1921 ctgacgcgcc ggtgctgcct tttttctcct gccacatgaa gcacttcact gattttcgca
     1981 atcgtggcaa catagtaagc cagtatacac tccgctagcg ctacatgact ggttcagggc
     2041 tgcgccccga aacccgctaa caacctctca gctactggat atcaccccat gccatctgaa
     2101 tatgatgaac aactggattc gactgatacg cagcttcccg gcgaatatga cctttttgat
     2161 ttaatggcgc aggttactga agagaacctg catgcccctg tggattttgg gtcgcctgtc
     2221 ggccgggaag cgttatagct ttttcgcttg cccgcgtacg tggtaatact ccggtattac
     2281 cttttatttc tttattttca tgtaattaca gtggattttt aatacccggg tattactcgt
     2341 gaaaacgaaa tattcctttt ttttctttta attcatgcag ttgcgatgaa aatcgcggct
     2401 gcgttaggtg tatgaccatt ttgaggttga aaaataatca accatggcgg gtgatttacg
     2461 caggttaagg ggagatcgtg acaaaaaaag cgttcctcac tgtatcgttc atgcctgcat
     2521 gaacgggtta ttcttcctga ctacagaatt acatcgctcc ttcagttcat acatcacttt
     2581 tacatcactt atacatcact tttacatcac ttatacatca cttttacatc attccttcag
     2641 ctcatacgtc actttcatcc tctgtgccat ttgtacatca cccccttcct gaacgtgctg
     2701 acagcgggtt ttccggctgc gccggcaacg cttcacggcc acgcaataaa ttgcgtcccc
     2761 gttcaccgtt ttcggatggg aagaacgccg gaaacaggac ttaccccggc tgaatggtgt
     2821 gaatttccgc gctatgcact tgtgcgcata ctcatgcatg ccgtaaaaac agagcctgcg
     2881 cgtttctggc gggttttcgg gtggtttgtt gcctgtttta ccggtttccc gtcagaaacg
     2941 ccctgagggc ctctcaggcg gtgctgataa cagatgttat ggtaaatcag agtcacctgc
     3001 actgttactc tgaaccggag cgaaacagcc gcgaccagaa tccccgcttt ttttcctgtg
     3061 ccagcgcgtc agccagacgg ctaatttcgg cctgtagttg ttccctctcc tcatttcgca
     3121 ggcggttttc tgcttcccgc gccatcttgt cttcaagcat tagggtaaca gtctgctgca
     3181 gagcgtccag tttctgctga atgacgtcta gctgagcatt ttccgcctgg ctaacgacag
     3241 atttggagtg tccctccttg tgcctttttg gtgtctcaga agtgcgcagc tcgccatagg
     3301 cccgaatcag ttcactggtc tcgaactccc ggcgatcatt ttttcctgtg cgatagctga
     3361 caaggccttt agccatgtca cgatataatt gcgagcgcga ctttcctgtt aatttcagtg
     3421 tctcttgaac tgtgtgccat gccataaaag tgacagtgtc tcctgtgtgt accgtattgt
     3481 aacagcagaa catattactg ttttaacaca aaaggtctga ggctgcccgt gaggcgtgaa
     3541 aaatacacaa aaaacctgtt gacggtgtgg ttttgacggg cgtagtattt cgaatactgt
     3601 atataaacac attgttataa atacagtttt tgctgttttt aaaagtcaaa gaggataatt
     3661 ttatgagtac agagttaaat agcggtactg tcggtggttt tggtcacatg ggaccaaatg
     3721 acccctacaa tgatggtcat agcggctctg gaagttcggg aggtagtttt aacggtggtg
     3781 gtaatggtgg ccatgataat agtagtggtt catcttatac tgcatccttt ggtctcatca
     3841 ctggccgaca cccgataact gtcccaggta cgaagaatat taattctgtc gatctttctg
     3901 ctgtcattaa taacattata catcatgaag gtgcacgcaa taacatgtac ctagatactg
     3961 cgaacaatgt aagagtggga gtgacgggca ctggctggca atgtctagca acggcaggca
     4021 tttcggctga gggtaaaaga actttccgct aagcgataga ctgtatgtaa acacagtatt
     4081 gcaaggacgc ggaacatgcc tcatgtggcg gccaggacgg ccagccggga tcgggatact
     4141 ggtcgttacc agagccaccg acccgagcaa acccttctct atcagatcgt tgacgagtat
     4201 tacccggcat tcgctgcgct tatggcagag cagggaaagg aattgccggg ctatgtgcaa
     4261 cgggaatttg aagaatttct ccaatgcggg cggctggagc atggctttct acgggttcgc
     4321 tgcgagtctt gccacgccga gcacctggtc gctttcagct gtaagcgtcg cggtttctgc
     4381 ccgagctgtg gggcgcggcg gatggccgaa agtgccgcct tgctggttga tgaagtactg
     4441 cctgaacaac ccatgcgtca gtgggtgttg agcttcccgt ttcagctgcg tttcctgttt
     4501 ggggtcgttt gcgggaaggg gcggaatcct acgctaaggc tttggccagc gatattctcc
     4561 ggtgagattg atgtgttccc aggggatagg agaagtcgct tgatatctag tatgacgtct
     4621 gtcgcacctg cttgatcgcg gccgcgatag ctagatcgcg ttgctcctct tctccatccg
     4681 cgttccaagc tgcggaaagg cacccataag cgtacgcctg gtcgagcagg cgacgcggat
     4741 cgacgtccag cgcacgagag aatgcgtccg ccatctgtgc aatgcgtcta ggatcgagac
     4801 aaaggtcgtc tctgtcagcc ggatcgtaga acatattggc ggcgccaaag cccacttcac
     4861 cgaccagacc gacgggatct atcaccagcc agccgcgact ggagaacatg atgttttcat
     4921 gatgcagatc gccatgtagc ccacgcagtt ccgaggcatt gctcatcatt tgatcggcta
     4981 taatcgccgc gtggacgtag tcagtttgac aacctgcgtt ttgatcatcg cgcgcccgct
     5041 gaaacaaagc tgcaaagcga tcccggatcg ggagaagggc agaaggcagg ggttcctcag
     5101 atgcggcata cagcttcgcc attagttccg ctgcaatttc ggtcgcctgg tagtcgccgt
     5161 gctcggcaac gatgtgagag agcattcgct ccccggcata ttcgagcaac atcagattgt
     5221 tctcacgacc gagcaaccgg actgctcccc tcccattgcg ccataccaga tagtcggccc
     5281 cgcgcagttc atcagcaatg tcttctatag gtttcaatcc cttgacgatt gcaggagtcc
     5341 cgtctggcaa tgaaactttc caaacgaggc tggaaaaggt gtccgcaatg agaacaggtt
     5401 gcgaaacgtg ccaatgagca ggaaaaacag gcggcatgaa catcaacccc aagtcagagg
     5461 gtccaatcgc agatagaagg caaggcgttc gcggtcgggg gcttcgatcc ccaatacatt
     5521 gaataggaca gcgaaggcgc gctctgcttc atctggcgct gcccagttct cttcggcgtt
     5581 agcaatcatg agtgccaaat cggcatagcg atctgctgtt ccgagccgcc caaggtcgat
     5641 cagacccgtg cattgaagag ttttagggtc caccatgaag ttcggcatgc agggatcacc
     5701 atggcaaaca accatatcgt cgctatcaat cgcggaaaaa ggcgtagtgc gcgaagcgaa
     5761 cgaagctttt tgccgtcgat tgcatagctt tgttaaccct ttttccaaat ttgatagcaa
     5821 tagttaatgt ttgaagtaaa gtgttgctca aaaacaactt cgaaggtatt tggaatactc
     5881 gggaagaaaa catccccctc tggctcgatg tcgatcgtcg ataagtggag cgtagaggcc
     5941 atgggtaatg tttctcggta aatttctccg ccaccagaca ctataacgtg accggtgaat
     6001 tcagctagcc tgtccatggc ctcttcgatt gactgaaata caactacatt gtcatcattt
     6061 gatgtccaac ctgagcgggt aacgaccgcg tatttcctat tggggagtgc gcccatagat
     6121 tcaaacgtct tgcgacccac cagaagccac tgattgtagg tcaatgcttt aaaaagtagc
     6181 tgctcccctt tcgcggacca gggtatgtct ggaccgcaac caatcacgcc gtttttcgct
     6241 ttcgcagcta tcaatgatac tttcaaggtt ctcatcctgg gttaacggtg cgctcttggt
     6301 cgagccgcac cggtagctct agttcgacac gagccaaaag atcgagctgc ggcgtactct
     6361 tgtcctcgtc cggtaagaag tcgggattga cggcattgcg ggacaccaca tcaacggcgc
     6421 gtccgaacat tcgcgacagc ctgcgctcaa acggacattg atcaaccgat aggctgtgaa
     6481 cagcgccaag ttgctgcccc attgacggcc acgctttgag caaatccgct ccagacagat
     6541 cagccgccgg tactcccgga attgccgtta tcaccaagca tgcaccctcc tgttcctcct
     6601 gccagttgat gacctcgggg caagccacac ctcgaccttt gagccaaatg aggcggtcac
     6661 gctctccagc gagctcaccg cggcgggaag caggtgcgat tttcgcgaag gcatgcccgt
     6721 caccacgtcg aaaaacaaaa tcaccagatt ctccgcctct gacaggcaac cagtcagaat
     6781 gcgattcacc
//
LOCUS       DAJTMC010000196         6692 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.196, whole genome shotgun sequence.
ACCESSION   DAJTMC010000196 DAJTMC010000000
VERSION     DAJTMC010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6692
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.196"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            154..462
                     /locus_tag="OO130_003720"
     CDS             154..462
                     /locus_tag="OO130_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HCR5366957.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(521..1615)
                     /gene="yaiW"
                     /locus_tag="OO130_003721"
     CDS             complement(521..1615)
                     /gene="yaiW"
                     /locus_tag="OO130_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HCR5366958.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(1628..2848)
                     /gene="sbmA"
                     /locus_tag="OO130_003722"
     CDS             complement(1628..2848)
                     /gene="sbmA"
                     /locus_tag="OO130_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HCR5366959.1"
                     /translation="MFKSFFPKPGAFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLVA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            3200..4357
                     /gene="ampH"
                     /locus_tag="OO130_003723"
     CDS             3200..4357
                     /gene="ampH"
                     /locus_tag="OO130_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HCR5366960.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(4358..4981)
                     /gene="iprA"
                     /locus_tag="OO130_003724"
     CDS             complement(4358..4981)
                     /gene="iprA"
                     /locus_tag="OO130_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HCR5366961.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNSEDT
                     FFILEGVISLRREENVLIGITQASYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
     gene            complement(5069..>6692)
                     /locus_tag="OO130_003725"
     CDS             complement(5069..>6692)
                     /locus_tag="OO130_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR5366962.1"
                     /translation="NNGSQVNVTDSSLVSDTIGLTNGSSLNIGANGVVATDHLTVDSY
                     STVNLTESTGWNNYSNLYTNTITVTNGGVLDVNVDQFDTEAFRTDKLELTSGNIADHN
                     GNVISGVFDINSSDYVLNADLVNDRTWDTSKSNYGYGIVAMNSDGHLTINGNGDVDNG
                     TELDNSSVDNVVAATGNYKVRIDNATGAGTIADYKDKELIRVNDANTNATFSAANKAD
                     LGAYTYQAEQRGNTVVLQQMELTDYANMALSIPSANTNIWNLEQDTVGTRLTNSRHGL
                     ADNGGAWVSYFGGNFNGDNGTINYDQDVNGIMVGVDTKIDGNNAKWIVGAAAGFAKGD
                     MNDRSGQVDQDSQTAYIYSSAHFANNVFVDGSLSYSHFNNDLSATMSNGTYVDGSTNS
                     DAWGFGLKAGYDFKLGDAGYETPYGSVSGLFQSGDDYQLSNDMKVDGQSYDSMRYELG
                     VDAGYTFTYSEDQALTPYFKLAYVYDDSNNDNDVNGDSIDNGTEGSAVRVGLGTQFSF
                     TKNFSAYTDANYLGGGDVDQDWSANVGVKYTW"
BASE COUNT         1689 a         1716 c         1602 g         1685 t
ORIGIN      
        1 ttttgtgact cacaacgaag attcacgctt cgggaatatt cccaacatca atttaccatg
       61 attctgatat tgacaatcta aatgacctgt cagacttgcc ataagccgct ggtatcacac
      121 cagaagagaa gcagatgact tccggaggtt tttatggctg atttcaccct gtcaaaatcg
      181 ctgtttagcg gaaaatatcg caatgcctct tcaacgcctg gcaacattgc ctatgcgttg
      241 tttgtgctgt tttgcttttg ggctggggcg caattgctga acctgttagt gcatgcgccc
      301 ggcgtctatg agcgtttaat gcaggtccag gaaacaggtc gcccacgggt ggaaattggt
      361 ttaggtgtcg gcaccatttt cgggctgatc ccgtttttag taggctgcct catttttgca
      421 gtggtggcgc tatggctgca ctggcgacat cgccgccagt aagatttacg ttgccagata
      481 cggcctaaaa cgccttatct ggcatgacat tcctgtttga tcactgtttc atacaacgcg
      541 cccgccgttc gtctacgcgc ttcgcaaacc aggccgtagt caggttgcgg gtgattttcg
      601 ggctttccag ttgaatgcca ggcaacattt cgcggggtag tgatttaccc gttttcgctt
      661 cggcaagttg ataaactttc ttgtacagcg ccgtctcctc aaaagagaag ctgtcacctt
      721 tctctaactg acggcgaatt tcgctgtcgc tcatccccag ttttcccgcc agtttgcgcg
      781 ttgccagttc ggttttcccg ggttctttac tgtcgtaacg aattaaatca ccgtccagtg
      841 ccagcttcac gccgctggcc ttactgaccg cgttctgaaa cgcggcatta cggctggcgt
      901 accagccagc gttaaaatca gcaaagcggt atatcggtgc gctatagctg gcgggataat
      961 tcagtaaatg gtaagtacca aaccacaacc cgccgcggcg actgaagact tcctgacgaa
     1021 ctgtaccgtc cattttccac ggataccctt tggtatgctg ttcggcaaaa gcaatgctga
     1081 cctgcatcgg cccaccggtg cgcaccggat taagcgaacc aaacagcgtc tgccccattg
     1141 gtaccatgct gatcagatcg tcaaaaatcg cgctcaattg cttttctgta cgcaccgaat
     1201 ctaaccgttc gctataactt ttgccgtttg gcgatttgat ttttagcgcg gtatgaacca
     1261 gaaaagcagg aatgtgcatc cgttcagcac gacggtcaat ctcttgccag gcgatcttgc
     1321 ttaagcccgg aacggccgga tctgcctgat aatttgactc ctgctgcgcc accgccagca
     1381 ccgagcagac gttttccagc gtcggtgcca gcccctgact ttcaaaggtg gtggcgagat
     1441 ctttcgccca ggcatcgcgg tctttcacgc tcgcaggcat cttctgccgc acgacgctcg
     1501 ctacatctat cgctttttcg ccctttttca acggttgcgg agcctgacta ctacaccccg
     1561 ctaaaaccag aaccgccaac aatgacagcg aagatagatg attgacgcgc gacattactt
     1621 ctccttttta gctcaaggta tgggttactt cctgaatttt gtcaccatcc agctcatgtt
     1681 caaagctgcg cagacgtttg tagatagaca tcaactcaac cagtgtggtc catgagttaa
     1741 tcaggtactg gaaagcaccg cgaacctgac caaaaacgtt ggtaatctgc gtcatcaggc
     1801 cgagcgtaat cgtaccggca acaattgacg gaaacagcaa gaacaaaccg aaaacgttat
     1861 cgacctgcaa atagagaatg cgggcgatgt tgaaatacat atagtgaaaa tagaggcgaa
     1921 aatagttttt ccgtacggcg ctaaacagct cgcgtaccgt aggcggcgtc gcgcgcgtgg
     1981 catcgtcttc accataaacc agctctttac ggtaggcggc ctctactcgc tggtttttaa
     2041 actccagccc cggcagtttg atccctacca ctgccagcaa tccggtcccc atcagcgacc
     2101 agacgatagc ggcaatcacc agaccatacg gaatgtgccc gataataggc agctccggca
     2161 catgcgcgga gagcgttacc agcaccggca ggaaggcgac caacgtcatg atggcgttga
     2221 taaaactgac gcccatattc tccagcgttg aagcaaaacg catggtgtct tcctgcacac
     2281 gctgtgcggc accttcgata tgacgcagtt gttgccagtt cgccatgtaa tattcgttca
     2341 tcgccgtacg ccagcggaac acatagtgac tgacaaagaa gttgttcagt acactgatca
     2401 ccaccgcgat cagcgcaatc cccagaaaga cgcccacttc gcggtaaaat tgttcgatag
     2461 tgactttatg cggcgaactt agcgcggttt gaatcagatc atagaacggt gcataccagg
     2521 cgttgacggc gactccgact tccaccaaaa accaggtgac gaagatgatc agtgcagtac
     2581 cgagtattga ccagtattgc caacggtgcg ggctgtagat aaaccagaaa aatgcaaaaa
     2641 gtcctacgca aacaatgtag taagcgtaaa aaatcaggaa atccaacgac cagaaacgcg
     2701 cggcgctaat cgggatctgc ccggaagcgc cggtgatacg cgccacccag tctcccccac
     2761 cggcttgcca gaagataacg gcaatcaatg cccaaacaaa ggccgagaga aaaaacgctc
     2821 ccggctttgg gaaaaaagac ttaaacatat gcactcctgc taacttctta tcgttttaac
     2881 aactgctgtg tattaaccgc atgaccacta caaagcgtca gccaaatgaa accgcccatt
     2941 gtgacccata gcaaggcgct tagttcgcca gcctgaccat aagaattgtt tcgacggatt
     3001 tcacaaacca aagattgttt acataagcgg attaacacgc gcaggatcac aaatgcaggc
     3061 attggtgagc aggaaaaaga tcgtgtttac gcgccgttac gtttttaaca ctattattgc
     3121 actaattcgc cctttgcaat ccatcaatga gtagtataaa tacgctcagt taccttcatt
     3181 caatctatgg acaccaccgt tgaaacgtag tctgcttttt tctgccgtgc tgtgtgcggc
     3241 gtcattgacc tccgtccatg cggcgcagcc gatcactgaa ccggagtttg cctctgatat
     3301 tgtcgatcgt tatgccgatc atatttttta cggcagcggt gccacgggga tggcgctggt
     3361 tgttatcgac ggtaaccagc gcgtctttcg cagttatggc gaaacgcgac ctggtaataa
     3421 cgttcgcccg cagctggatt cggtcgtgcg tattgcttcc ctcaccaagc taatgaccag
     3481 tgaaatgctg gtgaaattgc tcgaccaggg gaccgtgaag ttaaacgatc cgctaagtaa
     3541 atatgctccg ccaggcgcac gcgtgccaac ctacaatggg acgcccatta ccctagtgaa
     3601 tctggcaacc cataccagcg ccctgccccg tgaacagccc ggtggcgcgg cacatcgtcc
     3661 ggtgtttgtc tggccaacgc gcgagcaacg ctggaaatac ctttctacgg cgaagctgaa
     3721 agccgcgcca ggcagtcagg cggcgtactc taaccttgcg ttcgacctgc tggcagatgc
     3781 actggcgaat gcgtccggca agccttatac ccagttgttt gaagagcaga ttacccgtcc
     3841 attggggatg aaagacacca cctacacccc ctcaccggat cagtgccgcc gtttaatggt
     3901 tgctgagcgc ggtgccagtc cgtgcaataa cacgctggcg gcaattggca gcggcggcgt
     3961 ctattccacg cctggcgata tgatgcgctg gatgcagcag tatttatcat ctgattttta
     4021 tcagcgcagt aaccaggcag atcgcatgca gacgttgatt tatcagcgcg cgcagtttac
     4081 caaagtgatt ggcatggatg tccccggcaa agcggatgcg cttggtttag gctgggtata
     4141 tatggccccg aaagagggtc gtccggggat tattcagaag acaggcggtg gtggcggttt
     4201 cattacctat atggcgatga tcccgcagaa gaatattggc gcgtttgtgg tggtcactcg
     4261 ttcgccgttg acgcgcttta aaaatatgag cgatggcatc aatgatctgg tgaccgagct
     4321 aagcgggaat aaaccgttgg ttatccctgc atcctgatta atactctgaa ggcaaacggt
     4381 tgatagcgac cagtttgcct ttattcattt cgatatagcc gcctttacgc aaagcagcga
     4441 gaacttctgc gacgacagaa cgcgatatgc gtgtacgttg atggatataa ttcatcacac
     4501 caatacgtga tcgcaattct tcattccagt caatcattga taataatgtt gcgcggattt
     4561 gttcgtagga attatgccca atgagctgca cgtcgcgtaa ttccagaatt ctattttgcc
     4621 aggctaacca gtaaaaagcg tctcgccaga gttgattttg ttcaataagc gtaatggttt
     4681 gtttggctgg tagatgatat cccgtacaat ttccttctga tattaatttg tatggtatat
     4741 cgtttttcat taaaccatca gccagcccca taatataaga agcctgggta ataccgataa
     4801 gtacgttttc ttctctacgc agagagataa cgccctctaa aataaagaaa gtatcttcgc
     4861 tattgacatc actggaaaat ataacttgct tttcattatt aaactcgaag cgcgtaccgt
     4921 atctggacaa acatttatcg agcttaccaa attcctgaag aggtttaact acagataaca
     4981 tttgcgcgtc ctttgcagta atgcccgtca aatccttgac gggcattatt tagattaaat
     5041 taccagaatt tcttcggagt gaagaatatt accaagtata tttaacaccc acgttcgcgg
     5101 accagtcttg atctacgtca ccaccaccga ggtagttagc atcggtatag gcgctgaagt
     5161 tcttggtgaa gctgaactga gtacccagac caacacgtac cgcagaccct tcagtaccgt
     5221 tatcgatgga atcaccgttc acatcgttat cgttgtttga gtcgtcgtag acgtaagcca
     5281 gtttgaagta cggagtcaga gcctgatctt cgctgtaggt gaaggtataa cctgcatcta
     5341 cacccagttc ataacgcatg ctgtcgtaag actgaccgtc aactttcatg tcgttgctca
     5401 gctggtagtc atcaccagac tggaacagac cagaaacgct gccgtaagga gtctcgtaac
     5461 cagcatcacc cagtttgaag tcgtaaccgg ctttcaaacc gaagccccac gcgtcagagt
     5521 tggtgctacc gtcaacgtaa gtaccgttgc tcatggttgc agacaggtcg ttgttgaagt
     5581 gagagtagct caagctacca tcaacaaaga cgttgttcgc gaagtgagca gaagagtaga
     5641 tgtaggcagt ctggctgtct tgatccacct gaccagaacg gtcattcatg tcacctttag
     5701 cgaagcctgc agccgcaccg acgatccact tagcgttgtt accgtcaatt ttggtatcaa
     5761 caccgaccat gatgccgtta acatcctgat catagttgat ggtgccgttg tcgccgttga
     5821 agttaccacc gaagtagctt acccatgcgc cgccgttatc agccaggcca tgacgagagt
     5881 tggtcagacg agtaccaacg gtgtcttgtt ccaggttcca gatattggtg ttcgcagacg
     5941 ggatgctcag cgccatgtta gcgtagtcgg tcagctccat ctgttgcaga acaacggtgt
     6001 taccgcgctg ttcagcctga taggtgtatg cacccaggtc agctttgtta gcagcagaga
     6061 aggtcgcgtt ggtgttagcg tcgtttaccc gaataagttc tttatctttg taatcagcga
     6121 tagtgccagc gccagttgcg ttgtcgatac gaactttgta gttaccggtt gcagcaacaa
     6181 cgttatcaac agagctgtta tccagttcag taccgttgtc tacgtcgccg ttaccattga
     6241 tagtcaggtg accatcagag ttcatagcaa caataccgta accgtagtta gacttggaag
     6301 tatcccacgt acggtcgttc accagatcag cgttcagaac gtaatcgctg ctgttgatgt
     6361 cgaacacacc agaaattacg ttgccattat ggtcagcaat gttgccgctg gtcagttcca
     6421 gtttatcagt acggaaagct tcagtatcga actgatcaac attcacatcc agcacaccac
     6481 cgttagtcac agtgatagta ttggtataca gattagaata attattccaa ccggtagatt
     6541 cggttaaatt aacggtactg tagctatcaa cagtcaggtg gtctgtagca accacgccat
     6601 ttgcaccaat attcagtgaa gaaccgttgg ttaaaccaat ggtatctgat accagagagg
     6661 aatcagtaac atttacctga gagccattat tt
//
LOCUS       DAJTMC010000197         6564 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.197, whole genome shotgun sequence.
ACCESSION   DAJTMC010000197 DAJTMC010000000
VERSION     DAJTMC010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6564
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.197"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            <1..357
                     /locus_tag="OO130_003726"
     CDS             <1..357
                     /locus_tag="OO130_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5366963.1"
                     /translation="AITALNFYQGDVPSVMIRIQPENMSPLIIDISTGEHDNYIIQTL
                     DVGTFAPFGEQCTCSAVNKKELECIKETISKYCAKFTQKEAILTAPTHFNKTCITSDC
                     WQILFFSPDHFNNDFY"
     gene            379..1557
                     /locus_tag="OO130_003727"
                     /pseudo
     CDS             379..1557
                     /locus_tag="OO130_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417591.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhaC family protein"
     gene            complement(1945..2321)
                     /gene="rnpB"
                     /locus_tag="OO130_003728"
     ncRNA           complement(1945..2321)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="OO130_003728"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(2354..3499)
                     /gene="garK"
                     /locus_tag="OO130_003729"
     CDS             complement(2354..3499)
                     /gene="garK"
                     /locus_tag="OO130_003729"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HCR5366964.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSVPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYAEVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTVL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            complement(3596..4486)
                     /gene="garR"
                     /locus_tag="OO130_003730"
     CDS             complement(3596..4486)
                     /gene="garR"
                     /locus_tag="OO130_003730"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HCR5366965.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(4516..5286)
                     /gene="garL"
                     /locus_tag="OO130_003731"
     CDS             complement(4516..5286)
                     /gene="garL"
                     /locus_tag="OO130_003731"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HCR5366966.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            complement(5302..6538)
                     /gene="garP"
                     /locus_tag="OO130_003732"
                     /pseudo
     CDS             complement(5302..6538)
                     /gene="garP"
                     /locus_tag="OO130_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005155249.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
BASE COUNT         1794 a         1705 c         1432 g         1633 t
ORIGIN      
        1 gcaattactg cattgaattt ctatcaaggc gatgttcctt ctgtcatgat ccgaatccaa
       61 ccggaaaata tgagtccatt aatcatagat atttctacag gagaacatga taattatatc
      121 atacaaacat tagatgtagg cacttttgca ccttttggtg aacaatgtac ttgctcagcc
      181 gtcaataaaa aagagctgga atgtattaag gagacgattt ctaagtattg cgcaaagttc
      241 acccaaaaag aagccatctt aacagcccct acgcacttta ataaaacctg cattacttca
      301 gattgttggc aaattctctt tttttcaccc gatcatttta ataatgattt ttattgatta
      361 tttataaagg ataaaataat gttccctgtt tcatcaattg gtaatgatat tagtagtgat
      421 ttagtccgtc gaaaaatgaa tgaccttcct gaaatcccca tagaaaataa tctcgaagca
      481 ctagcaccag gtatagaaaa actaaaacag acctctattc aaatggtcac tttacttaac
      541 gcgttacaac caggtggaaa atgtattatc actggtgatt ttcaaaaaga attagcgtac
      601 ttacaaaatg taattcttta taatgactcg tttcttcgtc tggatttttt aggttataac
      661 gcccaaatta ttcaacgatc ggacaatacc tgcgaactta ccattaatga accgttaaaa
      721 aaccagaaaa tatctacagg caatatcaat gttaattgtc cattaaaaga tatttacaat
      781 gaaatcagga ggttgaacgc aacctttagt tgtgggactg gagatatagt tgatctatcc
      841 tctctggact tacgtaatgt cgatttagat tattatgatt tcacagataa acatatggct
      901 aatactattc taaatccttt taaattggat tcaacaaatt ttactaatgc caacatgttt
      961 caggttaatt ttgttagttc aacacaaaac gccacaatct cctgggatta tttactaaaa
     1021 ataacgcctg ttttaataag tattagcgat atgtattctg aagaaaaaat caagtttgtc
     1081 gaaagttgtt taaatgagct tggagacatt accgaagaac aattaaaaat tatgagattt
     1141 gcaattataa aatctatacc aagggcaact cttacagata aattagaaaa tgaattaaca
     1201 aaagaaatat ataaaagctc atcgaaaatc aacaattgct tgaacagaat taaattacca
     1261 gagatgaaag aattctcatc aaaaaaaaac atatgattac atcgatataa tcattgaaga
     1321 ttatgaaaat atcaaagaaa atgcttatct ggtcgtcccc caaattaatt atactatgga
     1381 tttaaacata gaagactcca gctcagaaga gttaataccc tcgagaaaga cgaaaattct
     1441 tcggacaatg gctttgaagt cgaagaatat aacgcatatg aagcatataa ctcagagaat
     1501 ctatatttta ccagagagga atatacgtat gattacgacc tttcaaatgc aatatagact
     1561 taataacttc ttacaaaatc tataattaat accgtagata ttcattcatc ttaccctgtg
     1621 caccaaaagc aaaaactcac tgacgcgggt ttgaaactga tggcaaccgc aaaaatgcct
     1681 gatgcgctac gcttatcagg cctacgccat ctctgcaata tattgaattt gcgtgctttt
     1741 gtaggcagga taaggcgttc acgccgcatc cggcatgaat aaagcgcact caacaatctg
     1801 aaacccgccg gaacggttta ttacgtacat caggtgaaac tgaccgataa gccgctttct
     1861 tttgggtata gtgtcgtgga cagtcattca tctttctgcc cctccaaaag caaaaacccg
     1921 ccgaagcggg tttttacgta aatcaggtga aactgaccga taagccgggt tctgtcgtgg
     1981 acagtcattc atctaggcca gcaatcgctc actggctcaa gcagcctacc cgggttcagt
     2041 acgggccgta ccttatgaac ccctatttgg ccttgctccg ggtggagttt accgtgccac
     2101 ggactgttac cagccgcgcg gtgcgctctt accgcaccct ttcaccctta cctgatcccg
     2161 cttgcgcggg ccatcggcgg tttgctctct gttgcactgg tcgtgggttt cccccccagg
     2221 cgttacctgg caccctgccc tatggagccc ggactttcct cccctccgcc cgtctccccc
     2281 gaagaggacg acgacgaagc ggcgactgtc tggtcagctt cggcgcgcag tatagagggt
     2341 ttgcgcgccc ttgtcacccc gcgttgcgca ttccaatcgc cagtgtcgcg gcgatattac
     2401 gtgaagcacg gcagatattg tcataagccc cgcggaatgc ttcgtccaac gtacctatgc
     2461 tggtcaatac gctgaagacc gcatcaatgc catgctgatg tacaacgcca acatcatcgg
     2521 tcaggctacc cgcaatgcca atcaccggtt tatggtactt cttcgccacg tttgcgacac
     2581 caatcggtac cttgccgtga atactctggc tgtcaatacg cccttcaccg gtgatcacca
     2641 gcgtacaatc gtgaatatgt tcttccagat tcagcaccgt agtgacgatt tcaataccac
     2701 ttttcagttc cgcaccaaga aacgccatca gcgccgcgcc cataccgccc gcagctcctg
     2761 cgccggggac atctttcaca tcaacatgca gcgctttttt aatgacctcg gcatagtgag
     2821 agaggttatt gtccagctca acaatcatcg cttcactggc tcccttttgt gggccaaaga
     2881 tgcgcgatgc gccgttatcg cccaccagcg gattggtgac atcacaagcg acgcgaatga
     2941 cgcaatcttt taagcgcgga tcgaggccgg aaatatcaat atcattcaga gtattaagac
     3001 taccgccgcc aaaaccaatt tcattgccgt tggcgtcgca taatttcgcc cccagcgcct
     3061 gtaccatgcc tgcgccgcca tcatttgtag cgctgccgcc aatgccgata ataatgtttg
     3121 tcgcaccgct ctccagcgcc tgcaggatta actcgcctgt gccgcgtgaa gtggtcacga
     3181 gtggatcgcg tttttccgca ggtaccagct ccagcccact ggccgccgcc atttcaataa
     3241 acgcggtttt gccatcgccg gagatccccc aactggcatt cactttctcg cccaacggcc
     3301 ctgtaaccca ggcgtgacgt tcggaaccct gggtggctgc aatcatcgct tccaccgttc
     3361 cttcgccacc gtcggcaacc ggaacagaaa cgtactgtgc atcaggaaaa atttcccgaa
     3421 atcctttttc tatcgcctgc gcaacctcgc tggcagataa actttcttta taagagtctg
     3481 gggcgattac gattttcata cctatgcctg ttaccacatg acgccggagg gcgtttctct
     3541 tattcggcgt ggattccagg cccggattgc aatacgccat ccgggcacga cgtcattaac
     3601 gagtaacttc gactttcgcc agtttttcgt agtagcacgc cagagcgcta tgatccgccg
     3661 ttcctaaacc atctgcacgc agtgcctgca tcatctccat aaccgcagct gtgagcggca
     3721 gttgcgcgcc gacgccgtga gaagtatcca gcgcattcgc cagatcctta atatgcagat
     3781 caatgcggaa gcccggcttg aagttgcggt ccatcaccat cggcgctttg gcatccagca
     3841 cggtactgcc cgccagccca ccgcgaattg cctgataaac caggtccggg ttaacgcccg
     3901 ctttagttgc cagcgttaac gcttctgaca tcgcagcaat attcagcgcc acaatgacct
     3961 gatttgccag tttggtgacg ttacctgcac cgatttcccc ggtatgcacc acggaacccg
     4021 ccatcgcttt catcaaatca tagtatttgt cgaaaatagc cttgtcgccg cccaccatca
     4081 ctgacagcgt accgtcgatg gctttcggtt caccgccgct caccggagca tccagcatat
     4141 caatgccttt cgctttcagc gcttcgctga tttcacggct tgccagcggt gcgatagaac
     4201 tcatatcgat caataccgta cctggcttcg cgccttcaat aatgccattc tcacccagcg
     4261 ccacctcttt cacatgaggg gagtttggca gcatggttat gatgacgtcg cactgttcag
     4321 cgatcgcttt agccgtagac gctgtttctg cacctgcagc aatcacgtca gcaatagctt
     4381 ctgggttacg gtcagcaacc accagcgagt aacctgcttt cagaaggttt ttactcattg
     4441 gtttacccat aatccccagg ccaataaaac caactttcat agtcatatca atcatctctc
     4501 ttgttgcggt ggtggttatt ttttaaaggt atcagccagt ttctgagtgg cagagcggaa
     4561 gacgccaaga tcgctgccga cagccacaaa cgtcgcgccc cattccagat aacgacgcgc
     4621 atcggcttcg accggcgcga gaataccgct gggtttgccg tgcgcactgg cacggttaaa
     4681 aatatgctga attgcttttt gtacatccgg atgcgaggca ttgccgagat ggcccaatgc
     4741 cgcagccaga tcgctggggc cgacgaagat accgtctaca ccttcggtag cggcaatagc
     4801 atcgacgtta tctacgccct gctgactttc tatctggacc agaatagtga tgttcttgtt
     4861 cgactgagcg aaataatccg ccacggtgcc aaacatattg gcgcggtgag aaacggagac
     4921 gccgcgaatg ccttccggcg ggtaacgagt tgatgccacc gcctgctctg cttcctcttt
     4981 tgtttctaca aaaggaatca ggaagttata gaaaccgata tccagaagac gcttaataat
     5041 taccggctcg ttggtcggca ctcgcactac tggcgcgctg gcgctgcctt tcagggccat
     5101 taactgcgga ataaacgtgg aaatatcgtt tggcgcatgt tcgccatcca gcaccagcca
     5161 gtcaaaccca gccaaaccaa gaacttcagt gctaatcggg ttagagagtg ctgaccagca
     5221 accaatttgt acctgtttcg cagccagtgc ggctttgaat ttattcggga aaacatcgtt
     5281 attcatcgct tatacctttg cttatttctg caattccata cgtttaatgt cgccaactac
     5341 gaagaggtag cagaccatcg ccatcagcgc tgaacatccc acgaaaacca gtgctgcatt
     5401 gaaggagtgc agttcactta ccaggtagcc aatcaccagt ggagtgacaa tggaggcaac
     5461 attgccaaag acgttaaaga cgccgccaca gaggccaaca atctctttcg gcgcggtgtc
     5521 agaaatcacc ggccagccca gagcaccaaa tcctttgcca aaaaaagcca gcgccatcag
     5581 catgaccacc agcgtggtgt tgttggtgta gttacataag atgatggtgg aagccagcaa
     5641 cattcccagc acaatcggta gcttacgtgc cagggtcagg gataaaccgc gtttgatcag
     5701 ataatccgag aagacacctc ccagcacgcc gcccgcaaaa ccacacagtg ccggaatcga
     5761 ggcgaccaga cccactttca gaatcgacat gcctttttcc tgcaccagat aaatcgggaa
     5821 ccaggtgagg aagaaccagg tgatggtgtt gataaaatat tgtccgaaaa atacgcccaa
     5881 catcatgcgg ttagagagca attgcttgat gtaatgcagt ttgggaccgc ttgctgccgc
     5941 gctgcccggc tttttgtggt ccatatcgac caccgcgcca ttttcagaga taaacttcag
     6001 ctcttccgca gacatacgtg ggtgatctgt cgggttataa atcaacttga tccacagcgc
     6061 cgtcagcaca aaaccaatca cccccataac ggtgaagacg tgctcccagc cccaggcgaa
     6121 agtcagccag ccaagcagag gcgaaaagag cgccagcgag aaatattgcg ccgagttaaa
     6181 gatggcggag gcagtaccac gttctttcgt cgggaaccag gcggcgacaa tacgggcgtt
     6241 cgccgggaat gatggcgctt ccgagaagcc gagcataaag cgcataaaga acatagagat
     6301 ccctgcccag gccagcggga acatatcaac aaagccttgc aggaaggtga atagcgacca
     6361 gaaaaagagg ctgtaggtgt aaactttttt cgagccaaac ttatcaagca gccagccgcc
     6421 gggaatttgc atcagcaggt aggacttcgg taccagcaat agacagcgtt gcacaatcgg
     6481 cgtagttaac ggcggtaaca ataaaaataa tcagtaatat taaatagcgg gtatgcacgc
     6541 ctttcttttt tacgtcaacg gtgt
//
LOCUS       DAJTMC010000198         6393 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.198, whole genome shotgun sequence.
ACCESSION   DAJTMC010000198 DAJTMC010000000
VERSION     DAJTMC010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6393
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.198"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(<1..208)
                     /locus_tag="OO130_003733"
     CDS             complement(<1..208)
                     /locus_tag="OO130_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HCR5366967.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDN"
     gene            687..2465
                     /locus_tag="OO130_003734"
     CDS             687..2465
                     /locus_tag="OO130_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HCR5366968.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQLEVEIVDENLCRVAG
                     TGAYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDDESRVQFVNQTALKTLGVVQNNIIGKPIRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEVSEAHLSLDDAGGTALEEMEKQMIREALSRHNSK
                     KQVADELGIGIATLYRKIKKYELLNT"
     gene            complement(2505..2984)
                     /gene="xdhC"
                     /locus_tag="OO130_003735"
     CDS             complement(2505..2984)
                     /gene="xdhC"
                     /locus_tag="OO130_003735"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HCR5366969.1"
                     /translation="MNHSETITIECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(2981..3859)
                     /gene="xdhB"
                     /locus_tag="OO130_003736"
     CDS             complement(2981..3859)
                     /gene="xdhB"
                     /locus_tag="OO130_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HCR5366970.1"
                     /translation="MFDFASYHRAATLADAINLLANNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPITQRHLPALCAAAMSIAG
                     LQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(3870..6167)
                     /gene="xdhA"
                     /locus_tag="OO130_003737"
     CDS             complement(3870..6167)
                     /gene="xdhA"
                     /locus_tag="OO130_003737"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HCR5366971.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAIAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGTFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
BASE COUNT         1595 a         1511 c         1616 g         1671 t
ORIGIN      
        1 agttgtcgcg gaatacggaa atacctaatc cactattaaa gactttggtt gagatgtttt
       61 cagcacgcag tgcttttaat gctgcggcaa cgtccagtac ttgtttcagt tcatctggcg
      121 tctgttccca cgttaacaaa aaatctttct cgtgaaggtg agaggtcagc gaattgatat
      181 ccttaatcag ctcattaaca gttttcatca taaagtcccg gtagtggaat gacaggcaaa
      241 gtggtttatt tcactgtgac ttgccaaagt gattggtgtt ttcacagcta tacctgggac
      301 taacaataag tgtgccagaa ttatccgcgc ggctgcaaaa gactaatttg acggataatg
      361 tgtggggttg atcacaaatt gatgccagct tctggttgtt tgtaaaatac gaaaacgttt
      421 ggcttttttt atcgcgttat ttatttgctg ctaagtatca gaaataagat aaaggggcag
      481 gctatctctc ttcagtttga taaactcacc gccgtaattc tcatattgat atagaaatgt
      541 gatatctctt ccataatttc attagaaaat gataaaaatc ttatgttttt atcaaagcca
      601 atttcgcctg agttaatatc atcatgatct gtgaaccatc aacgtcttca atgatcgact
      661 tttaagtaag tctggaaggt aaagctatgg agcttgctac tacgcagtca gtattgatgc
      721 aaattcaacc gacaattcag cgttttgcca gaatgcttgc cagcgttttg cagcttgagg
      781 ttgagatcgt tgatgaaaac ttgtgtcgcg tcgccggaac gggcgcgtat gggaagtttc
      841 ttggtcgcca gttgagcggc aactcacgcc tgctccgcca cgtcctggaa acgaaaactg
      901 aaaaagttgt gacacagtct cgcttcgatc ccctttgcga aggttgcgat agtaaagaaa
      961 attgccgtga aaaagcattt ctgggtacgc ctgtcatttt acaggatcgt tgtgttgggg
     1021 tgataagttt gattgccgtt acccacgagc aacaagagca tatcagtgat aatttacgcg
     1081 aattttctga ttatgttcgc catatatcca ccatttttgt ttcgaaactt ctggaggatc
     1141 aggggccagg agataacatc agtaaaatat tcgcgaccat gatcgataat atggatcagg
     1201 gcgtattagt tgttgatgat gaaagtcggg ttcagtttgt taatcagact gccttaaaaa
     1261 cacttggtgt tgtacaaaat aatattattg ggaaacctat ccgtttcaga ccattaacat
     1321 ttgagagtaa ttttactcat ggacatatgc agcatattgt ttcgtgggac gataaaagtg
     1381 aattaatcat tggtcaattg cataacattc agggccgaca attattttta atggcatttc
     1441 accaatcgca taccagtttt tctgtagcaa atgcacctga tgaaccacat attgaacaat
     1501 tggttggcga gtgccgtgtt atgcggcaat taaaacgact cattagccgt attgcaccca
     1561 gcccatccag cgttatggtg gttggtgaaa gcggcacggg taaagaagtc gtcgcccgag
     1621 caatccataa gttgagcgga agacggaata aaccctttat tgctatcaac tgtgccgcga
     1681 ttccggagca gcttctggaa agcgaactgt tcggttatgt taaaggcgca tttactggcg
     1741 cttctgccaa cggtaaaaca gggttgattc aggcggcgaa tacgggcacg ctgtttctcg
     1801 atgaaatagg tgatatgcca ttaatgttgc aggctaaatt actgcgcgct attgaggcgc
     1861 gtgaaattct gccgattggt gccagtagcc caatacaagt cgacattcgc atcatttctg
     1921 caactaatca gaatttggcc cagttcattg ccgaaggtaa attccgcgaa gatctcttct
     1981 accgacttaa tgttatcccg ataactctgc caccgctgcg tgaacgtcag gaagatattg
     2041 aactattggt gcattacttt ttacatctgc atacccgtcg tctgggatcg gtttatcctg
     2101 gcattgctcc cgatgtcgtc gaaatattgc gtaagcatcg ttggcccgga aacctgcgcg
     2161 agttaagcaa tttgatggaa tatctggtta acgtagttcc ttcaggtgaa gttatcgaca
     2221 gcacgctatt gccgccaaat ctgctgaata atggcacaac ggagcaaagt gatgtaacag
     2281 aggtcagtga ggcgcacctg tcactcgatg atgcgggcgg cacggcgctg gaggagatgg
     2341 aaaagcaaat gatccgcgag gcgctttcac gtcataacag caagaagcaa gttgctgatg
     2401 aactgggcat cggcattgct acgctctatc gcaagattaa gaaatatgag ttgttaaaca
     2461 cataacctca gtgaatccgg cctgaattca ggccggatat ccttttactt cgttttctcg
     2521 caatccagaa ctgtatttac aatcatctga taccccgtgc agcgacaaag atttcccgcc
     2581 agtccgcgac gaatttccgt aatggttaat ggcttctcgc gtggtttcgc cagcattgcc
     2641 gtggtagcca taatcaggcc aggcgtacaa aacccgcact gcactgcgcc ggatttcgca
     2701 taagcctgct gaacatgaga aagttttccg cctttcgctt caccttccag cgtgcggatc
     2761 tcttttcctt cagcccaggc ggcaaggtat aagcaactgt ctattgctgt gccgtcgacc
     2821 aacaccgtac aggcaccaca ttcacccacg cagcaccctt gtttgacact tagcagtcct
     2881 tgttcgcgga gtaattccga gagcggcgtg cctggtgcgg cgtgaagctg aaaaggcatc
     2941 ccgttaatgg tgcattcgat ggtaattgtt tcgctgtgat tcattgcaat tttccccccg
     3001 ccgcggcgac ggcttcgcta atcacttttt tggtcatcgt ctggatgaga tgcagacgaa
     3061 actctttact ggcccgccat gaagaacgcg gggcgacatc ttgcaggaca gattcgctga
     3121 tagcttccag cgtttgcagg tttaatggcg cattttgtgc agtctgttcg gcatgttggc
     3181 agcgaatcgg cgttggcgcg gcaacaccaa atgccaggcg taattcgctg aaattgccgt
     3241 tatccagtcg gcaatgtgcg gcgcagccaa tcgttgaaat atccattgcg tcgcgcatgg
     3301 catatttaaa atgcgcgctg cccgcgtgtt ctttcggctg tggcggaaaa tgaaaggcga
     3361 cgaggatttc gtcatgctca agagacactt tgcccggccc ggtgtgaaag ccattaatcg
     3421 ggacgaaacg aacaccgcgc ggggagtgga tctccagttt cgcgtcataa attagcgttg
     3481 gcgtggcaga atctgcgctg gtggcaccgt tgcaaatatt tccaccgtag gtagcgacgt
     3541 tacggatctg cagtccagca atggacatgg ccgcagcaca taacgccggg agatgacgtt
     3601 gagttatagg atcttctatt agctgggtaa atgtcgttgc agagccgata cgtagcgagc
     3661 catcttccgc cagcgtaatt ccccgcagct ccgccagatt atgaatatca acaatatggc
     3721 gataacggtc attgtgatgg tggagctgaa tcagtacgtc agtgccaccg gcgagcagtt
     3781 tggcctgcgg gttgttagcc agcaggttga tggcatcggc aagggttgct gcgcgatggt
     3841 aagaagcaaa atcaaacatg atgttatcct caaatcaatc ctgccagatg gaactcttca
     3901 tataaccgtt ttggcgtcag cggcagtgta ttgattgcaa caccggtagc catcttcacc
     3961 gcgttacgaa tagcagcggc aacaggaatt attggtggct cacccagtga cttatgtccg
     4021 tatgcggatt gcggctcatt gatttcgacg aacgcgcttt ccagttgtgg cagatccggc
     4081 atggtcggca ttttgtaatc cagcagattg gggttacgga ccacgccgct tttagcatcg
     4141 atgatcatct cttcaaatag cgcccagcca atgcccattc ccattccgcc gtgtacctga
     4201 ccttctgcca gcagtggatt aagaatatgc cctgaatcat gaacgttgag gatgcggttg
     4261 atggtgactt tgcacagcgc aatatcgacc gtcagatcaa caaaggtaca gccaaacgcc
     4321 ggtgggttag tggtggtttt gatggagctt tcagcagaga gctgcccgcc gcgttcaggg
     4381 tggtagaaag cgtccatcgc caaatctttt aacgacatta acggctcttc cggtcgttca
     4441 accagcacga tatggccttt tatcagggtc agattcatcg ctgactgatg tagcatgact
     4501 gcggcgtgag cgatgatttt ctcttttaat aatagtgccg cactgcgcag cgcaggcgcg
     4561 gcaacatagc tctggcgtga ggcaaatgtg ccgggatcga acggcgtaac gtcggtatct
     4621 tgagttgaaa taacgcgaac gtcgctgacc ggaaccccca cggtttctgc caccatttgc
     4681 gagaagacgg tgtcggcacc ctgaccgatt tccgtcgcgc cgctttgcac gttgatggtt
     4741 ccatcctgat tcatcagaag gcgcgcgcct gctatttcta cgccgacagg ccaggtgtta
     4801 gaggtgtagc taaaacaggc gacgccaacg ccgcggcgca aattgccttg ctggttctgg
     4861 cattctgcac ggcgtttttc ccattcaaag attttccggc ctttttcaag acactccggc
     4921 aaccctgcgc tgtaaatacg tttgcccgtg agcggattag catctccttc gcgggcggcg
     4981 ttgcgtaaac gaatttcaac aggatcaata cctaacgctg tcgcggcgtc atcaagcata
     5041 gactcaacgg caaatacgac ttgtggcgcg ccataaccac gcatcgcacc agccgagggg
     5101 aggttggtat agcaggtctt tgaactgtaa gcgtaggcac aacgaggata aaggtaagcg
     5161 actttattcc cccccgcaga agcgatggag tgcccgtgag atgcataagc gccggtgtta
     5221 gacagaacat ccagactata acctttcaat gttccgtcgc ggttcacgcc catttgcccg
     5281 tcaatggtaa aagcgtggcg ggtacgggtt gcgaggaaac actcttcacg gctaagggaa
     5341 actttcaccg gaatgccgcc aagcttgctg gtcaggaatg ccgccattgg ctcttccagt
     5401 acatcctgtt tattaccaaa accgccaccg acaaatggtt tgatgactcg tacgcatgac
     5461 cagggaatat ccagcgcctg accaaccacg cggcgaacaa tgtgcgggat ctgggtgctg
     5521 gaaacgatgg taattcgcga gtcatcctcc atccacgcca gcgatgttac gctttccatg
     5581 tgacaatgtt gaataacggg ggtctgatag tgcccctgta cctggtagtc ggcggcatcg
     5641 attgtttgtt ggacattacc cgtcgacatc gtgctttgtt tcagtaaatt gccaccgtta
     5701 tggattggtg cagcgtcttc tgccagcgcc gcttctggcg tggtgataac gggtaattct
     5761 tgccactcaa tgctgaccaa ttgcgccgct ttttctgccg tgagttcatc gcgggccacg
     5821 acgatggcaa tggcgtcacc atgatgacga acatggcgag ttagcagtgc gcgatcggcg
     5881 gtatcgcgct tgttttcgtc aagtgtccag gcatgcccag ctgtagcgaa tggaatatca
     5941 ggcacatctt cccaggtaaa aatcgccagt acgcccggca aacttctggc ttgttcatca
     6001 ttaatactta cggcataacc atgtgcgata gggctacgta catatttcgc ataacacatg
     6061 cccgccataa cataatcgtc agtatatcgt gcccgcccgg tgaccttagc aatggcatcg
     6121 acgcgcatgc atgattcacc cgtagcggtt gcttcccgcg cttccataga aatcccctcg
     6181 atttgaacat ttggttctta tggcaaagat ttagtctaag caggcaagat ttacgccaga
     6241 aacgacgata agcgttaggg gaggtaggaa gtgtgatgac gttaaaaaaa cacagatatg
     6301 atacgaaaca ggagtctgaa taggggcagt gatacgtatc aatatgagtg gcgataaaat
     6361 caatgtgata aattgggatt attacgcatc agg
//
LOCUS       DAJTMC010000199         6337 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.199, whole genome shotgun sequence.
ACCESSION   DAJTMC010000199 DAJTMC010000000
VERSION     DAJTMC010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6337
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.199"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            30..152
                     /locus_tag="OO130_003738"
     CDS             30..152
                     /locus_tag="OO130_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001124879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HCR5366972.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDT"
     gene            168..1013
                     /locus_tag="OO130_003739"
     CDS             168..1013
                     /locus_tag="OO130_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCR5366973.1"
                     /translation="MRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDA
                     IILSAVSENGSSRTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLSN
                     AAADYFIANKIDQPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLDK
                     AISVGEKLIISTPDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELE
                     NNQVLKAVVDISGKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATH
                     VDGLP"
     gene            1036..2547
                     /locus_tag="OO130_003740"
     CDS             1036..2547
                     /locus_tag="OO130_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCR5366974.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGGRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKITRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            2572..3570
                     /locus_tag="OO130_003741"
     CDS             2572..3570
                     /locus_tag="OO130_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5366975.1"
                     /translation="MSASSLSLPLGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            3603..4697
                     /locus_tag="OO130_003742"
     CDS             3603..4697
                     /locus_tag="OO130_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCR5366976.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALRSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYALMASGKCGKVVINFPD"
     gene            4709..5581
                     /locus_tag="OO130_003743"
     CDS             4709..5581
                     /locus_tag="OO130_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HCR5366977.1"
                     /translation="MTIYTLSHGPLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(5629..6051)
                     /locus_tag="OO130_003744"
     CDS             complement(5629..6051)
                     /locus_tag="OO130_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCR5366978.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIILGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(6148..>6337)
                     /locus_tag="OO130_003745"
     CDS             complement(6148..>6337)
                     /locus_tag="OO130_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phenoxybenzoate dioxygenase"
                     /protein_id="HCR5366979.1"
                     /translation="WLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSG
                     KTFDAKLLIDENIALKSL"
BASE COUNT         1467 a         1515 c         1836 g         1519 t
ORIGIN      
        1 ttgttaataa aaaccctaca ggaggcacta tgcctaaaaa aatgagaaca acccgtaatc
       61 tattattaat ggctacgcta ttaggtagcg cactatttgc cagggctgcg gataaagaaa
      121 tgaccattgg tgcaatatac cttgatacct agggatatta cgctggagtg cgtcagggcg
      181 ttcaggatgc ggcaaaagat tcttcagtac aggtacagtt aattgaaact aacgcccagg
      241 gtgatatttc gaaagaaagt acctttgttg ataccctcgt ggcgcgtaat gtcgatgcca
      301 ttattttatc ggcagtgtct gaaaatggca gtagccgtac cgttcgtcgc gccagtgaag
      361 cgggtattcc ggtgatttgc tacaacacct gtattaatca aaagggtgtc gataaatatg
      421 tctcggcgta tctggtcggc gatccactgg aatttggtaa aaaactgagt aacgctgccg
      481 ccgattattt tattgccaat aaaattgacc agccgaaaat tgccgtcatc aattgcgaag
      541 cctttgaagt ttgtgtgcag cgacgtaaag gatttgaaga agtattaaaa tcccgcgttc
      601 ccggcgcgca aattgtcgct aatcaggaag ggactgtttt agataaagcg atttccgttg
      661 gtgaaaaact gattatctcc acgccggatc tcaacgccat tatgggggag tcgggcggtg
      721 cgacactcgg cgcggtaaaa gcggtacgta atcaaaatca ggccggaaaa attgctgttt
      781 tcggttcgga tatgacaacc gaaattgctc aggagctgga aaacaatcag gtgctgaaag
      841 cggtagtgga tatttccggt aagaaaatgg gcaatgctgt tttcgcgcaa acattgaagg
      901 ttatcaataa acaagccgac ggtgaaaaag tgattcaggt gccaatcgat ctctatacca
      961 aaacggaaga tggtaaacag tggctggcaa cgcacgttga tggtctgccc taatcgctga
     1021 ttcgcagagg tccctatgtt cacggcaaca gaggcagtcc cggtagcaaa agtggtggca
     1081 ggaaataagc gttaccccgg cgtcgttgcg ctggataacg ttaacttcac gctcaataaa
     1141 ggcgaagttc gtgcgctgtt aggtaaaaac ggcgcgggca aatcgactct cattcgaatg
     1201 cttaccggca gcgaacgccc ggatagcggt gatatctgga ttggcgagac gcgactggaa
     1261 ggtgacgaag ctacgttgac tcgccgtgcc gctgaactgg ggggtcgtgc ggtttatcag
     1321 gaattaagtc tggtggaagg gctgacggtg gcagaaaacc tctgccttgg tcaatggcct
     1381 cgccgcaacg gcatgattga ttacctgcaa atggcgcagg atgcccaacg ttgcttacag
     1441 gcgctgggcg ttgacgttag tcctgaacaa cttgtttcaa cgctaagccc ggcgcaaaag
     1501 caactggtgg aaattgcacg ggtgatgaag ggcgagccgc gcgtggtcat tcttgatgaa
     1561 cccaccagct cgcttgccag tgcagaagtt gaactggtga tcagtgcggt gaaaaagatg
     1621 tcggcactgg gcgtggcggt gatttatgtc agccaccgga tggaagaaat ccgccgcatt
     1681 gcctcctgtg ccaccgttat gcgcgatggt caggtggcgg gcgatgtgat gctcgaaaac
     1741 acctccacgc atcatattgt gtcgctaatg ctagggcgtg atcacgttga tattgcgccg
     1801 gttgcccctc aggaaattgt ggatcaggcc gtactggaag tccgtgcgtt acgccataag
     1861 cccaagctgg aggatatcag ctttacgtta cgtcgcggcg aagtgcttgg cattgctggc
     1921 ctgctggggg cagggcgcag tgaattgctg aaggcgattg ttgggctgga gacgtatgaa
     1981 cagggcgaaa ttgttatcaa cggcgagaaa atcacgcgcc ccgattacgg cgacatgctg
     2041 aaacgcggca ttggctatac gccagaaaac cgcaaagaag cggggatcat tccctggttg
     2101 ggcgttgacg aaaatacagt gctgaccaat cggcaaaaaa tcagcgccaa cggagtgctg
     2161 caatggtcca ccatccgccg cctgaccgaa gaggtgatgc agcggatgac ggtcaaggcc
     2221 gccagtagcg aaacgcccat cggcacgctt tctggtggca atcagcaaaa agtggtgatc
     2281 ggtcgttggg tctatgccgc cagccagatt ttgttgctcg acgagccaac gcgcggcgtc
     2341 gatatcgaag ccaaacagca gatttaccgt attgtccgtg agctggctgc cgaaggaaaa
     2401 agcgtggtgt ttatctccag tgaagtggag gagttgccgc tggtgtgcga ccgcatcctg
     2461 ttgttacagc acggtacgtt ctcgcaggag tttcactcgc cggtcaatgt ggatgagctg
     2521 atgtccgcca ttctgtctgt gcactgatga attttaccga ggaacctgaa gatgtctgct
     2581 tcgtcattat cattgccgct gggcaagagc gtctcgctca aacaatttgt cagtcgccat
     2641 attaatgaga tcggtttgct ggtggtaatc gccattcttt atctggtctt ctccctgaat
     2701 gcgcctggct ttatctcatt gaataaccag atgaacgtgc ttcgcgatgc tgccaccatc
     2761 gggatcgccg cctgggcaat gacgctgatt attatctccg gtgaaattga tgtcagcgtt
     2821 ggaccgatgg tggcttttgt ctcggtgtgc ctggcatttt tgctgcaatt tgaagttccg
     2881 ttggcgattg cttgtctgct ggtgttgctg ttaggcgcgc tgatgggaac gctcgccggg
     2941 gtgctgcgcg gcgtgtttaa cgtgcccagt ttcgttgcca cactggggct gtggagcgcc
     3001 ctgcgcggaa tggggctgtt tatgaccaac gcactgccag tgccgattga cgaaaacgag
     3061 gtgctggact ggctgggcgg acaatttctc ggtgtgccag tatccgcgct gatcatgatg
     3121 gtgttgtttg cgctgtttgt gttcattagc cgaaaaaccg ccttcgggcg ctcggttttt
     3181 gccgttggcg gtaatgccac ggcggcgcag ttgtgcggta ttaacgttcg tcgagtgcgc
     3241 attcttatct ttaccctttc gggattatta gcggcggtga ccggcatttt gctggcggca
     3301 cgcctcggtt ccggtaacgc aggtgccgca aacggtctgg agtttgacgt catcgccgcg
     3361 gtcgtggtcg gcggtacggc actttccggc ggtcgcggtt ccctgttcgg tacattgctt
     3421 ggtgtgctgg tgattacgct aatcggtaac ggtctggtgc tgctcggtat taactccttt
     3481 ttccagcagg tggtacgcgg cgtcatcatc gtggtggcgg tgctggcgaa tatcttgctg
     3541 acccagcgaa gcagtaaagc gaaacgctaa accttaacgc ccctggcgcg ggtgcggcag
     3601 ggatgaaaat taatcgtatt gttaatgagg gatttatgaa aacgatgctg gcagcttatt
     3661 taccaggaaa ttcaaccgtc gatctgcggg aagttgcggt gccgacgccg gggattaacc
     3721 aggtactgat caaaatgaaa tcctccggga tttgcggaag cgatgtccac tatatctatc
     3781 atcaacaccg tgccacggcg gcggcacccg ataaaccgtt ataccaggga tttatcaacg
     3841 gtcatgaacc atgcgggcag attgtggcga tggggcaagg ctgtcgccat tttaaagagg
     3901 gtgatcgcgt gctggtgtat cacatttctg gctgtggttt ttgcccgaac tgccgccgcg
     3961 gctttcctat ctcttgtact ggcaaaggaa aagcggctta cggctggcag cgtgacggcg
     4021 gtcatgccga atacttgctg gcggaagaaa aagatctgat cctcctgccg gatgcgctta
     4081 gctacgaaga tggtgcgttt atcagttgcg gcgttggtac agcgtatgaa ggaattttgc
     4141 gcggcgaagt ttccggcagt gataacgtgc tggtggtcgg tctggggcca gtcggcatga
     4201 tggcgatgat gctggcgaaa ggtcgcggtg caaaacggat catcggcgtt gatatgctgc
     4261 cggaacgtct ggcgatggca aaacagttag gggtgatgga tcacggctat ttagcaacca
     4321 ccgaaggtct gccgcagatt atcgccgaac tcacccacgg tggcgcggat gttgcgctcg
     4381 attgttctgg taatgccgca ggtcgcttgc tggcactgcg atccaccgct gattggggac
     4441 gggtggttta catcggagaa accggaaaag tggagttcga ggtcagcgcc gatctgatgc
     4501 accatcaacg gcggattatc ggctcctggg tgaccagtct gttccatatg gaaaaatgcg
     4561 cccatgatct gacggactgg aaactgtggc cgcgtaacgc cattacccat cgcttctcgc
     4621 tggaacaggc tggagatgcc tatgcgctga tggcgagcgg caaatgcggg aaagttgtga
     4681 ttaacttccc ggattaagga gttagcggat gaccatctat accttatctc acggaccgct
     4741 aaagctggat gtctctgacc agggcggcgt aatcgaaggc ttctggcgtg acacgacgcc
     4801 gcttttacgt cctggtaaaa aaagcggtgt ggcaaccgat gcttcctgtt ttccgctggt
     4861 gccgtttgca aaccgggtga gcggcaatcg ttttgtctgg caggggcgtg agtatcaact
     4921 gcaaccgaat gttgagtggg atgcgcacta tctacacggc gatggctggc tgggcgagtg
     4981 gcaatgtgtc tcgcacagcg atgatagctt gtgtctggtg tatgaacatc gcagcggtgt
     5041 ctatcactat cgggtaagtc aggcgtttca tttaacggcg gatacgctga cggtgacgct
     5101 ctctgtcacc aatcaagggg cagagacgct gccatttggt accggctggc atccttattt
     5161 tccgttgtca ccacaaacgc ggattcaggc gcaggcgagc ggttactggc tggagcggga
     5221 gcagtggctg gcgggtgagt tttgcgagca gctaccgcag gaactggatt ttaaccagct
     5281 cgcgccgttg ccgcgccagt gggtgaacaa tggttttgcc ggatggaatg gtcaggcccg
     5341 catcgagcag ccgcaggaag gttatgccat catcatggaa acgacgccac ctgcaccgtg
     5401 ttatttcatc tttgtttccg accctgcgtt tgataaagga tatgcgtttg atttcttctg
     5461 tctggaaccg atgagccatg cgccggatga tcatcatcgc ccggaagggg gtgatctcat
     5521 tgcgctagca ccaggggaat caacaacttc agagatgtcg ttgcgggtag agtggctgta
     5581 atttattaaa tgccggataa ggcgttcacg ccgcatccgg catctttcct aacgccgatc
     5641 gagagaaatt gcccccggcc cggtaattgc caatagcaag aacgcgccag cgatactgac
     5701 attcttccag aaattaatca tatttggccc aaccgcatcg ccggtcatat cccagtaatg
     5761 gtgaccaatc accgccgtac ccagcgtgta gaaaataaac agcaccgcca gcggacgggt
     5821 gaaaaagcca agcacgatta atatcgcggc gggcacttcc ataactaccg caataatcgc
     5881 tgccagcatc ggcattggcg cgcccaacga ggccatatat tggaccgtac cgtcaaagcc
     5941 catcattttg ggaaaaccca aaataatgaa aattaagacc acggcgatac gggcaattaa
     6001 taacaaaacg gggcgggcag ctccaaaatc aaaataacgt agtgtgttca taaccaatcc
     6061 ctttgcaaga taatgtgatt taaacgtaat acacactctt gcacctcgcc acgccccagg
     6121 cgttgagata ttcgctaatt atcctggtta cagtgattta agcgcgatgt tctcatctat
     6181 cagcagtttc gcatcaaacg ttttgccgct ctggatccat ttgcgaattg ggcgaatctc
     6241 acgcccctga ttcagcgtga ccgcaccgat aagcacgccg ttttgcagat taaaccaaat
     6301 cgccttctga gtttccgggt tgccacgaca aagccag
//
LOCUS       DAJTMC010000200         6317 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.200, whole genome shotgun sequence.
ACCESSION   DAJTMC010000200 DAJTMC010000000
VERSION     DAJTMC010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6317
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.200"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            285..626
                     /gene="raiA"
                     /locus_tag="OO130_003746"
     CDS             285..626
                     /gene="raiA"
                     /locus_tag="OO130_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HCR5366980.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    729..854
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            730..777
                     /gene="pheL"
                     /locus_tag="OO130_003747"
     CDS             730..777
                     /gene="pheL"
                     /locus_tag="OO130_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HCR5366981.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            877..2037
                     /gene="pheA"
                     /locus_tag="OO130_003748"
     CDS             877..2037
                     /gene="pheA"
                     /locus_tag="OO130_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HCR5366982.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGLADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQQQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(2080..3201)
                     /gene="tyrA"
                     /locus_tag="OO130_003749"
     CDS             complement(2080..3201)
                     /gene="tyrA"
                     /locus_tag="OO130_003749"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HCR5366983.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLKQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(3212..4282)
                     /gene="aroF"
                     /locus_tag="OO130_003750"
     CDS             complement(3212..4282)
                     /gene="aroF"
                     /locus_tag="OO130_003750"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HCR5366984.1"
                     /translation="MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDLETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4490..4855
                     /locus_tag="OO130_003751"
     CDS             4490..4855
                     /locus_tag="OO130_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HCR5366985.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFGDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVESASQ
                     LHEVWQKGADENASTIRLN"
     gene            5005..5523
                     /locus_tag="OO130_003752"
     CDS             5005..5523
                     /locus_tag="OO130_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HCR5366986.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAKQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            5513..>6317
                     /gene="dgcN"
                     /locus_tag="OO130_003753"
     CDS             5513..>6317
                     /gene="dgcN"
                     /locus_tag="OO130_003753"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HCR5366987.1"
                     /translation="MMDNDNSLNKRPTFKRALRNISMTSIFITMMLIWLLLSVTSVLT
                     LKQYAQKNLALTAATMTYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNIL
                     ASWHYTRKDPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSL
                     AVLTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFH
                     RFALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGIN"
BASE COUNT         1717 a         1612 c         1419 g         1569 t
ORIGIN      
        1 aaaaagagcc agtcagttgc ttaactgact ggcattacag cccttgagct gccgtttttt
       61 tattctgtta gttgtgaaac tgaagcgatt tagtcactat cgatctcatc aaatatggct
      121 cgctttgaga tattcctcaa gtaaaaaaac acctcttcct gcgatttctc acaaaaaaga
      181 ttcgttgaca aaaagtgaca aaattatgag attcccatca cacattttga catcaggaac
      241 ggtatgctga attcaccaag acgggaagac aagaggtaaa atttatgaca atgaacatta
      301 ccagcaaaca aatggaaatt actccggcca tccgccaaca tgtcgcagac cgtctcgcca
      361 aactggaaaa atggcaaaca catctgatta atccacatat cattctgtcc aaagagccac
      421 aagggtttgt tgctgacgcc acaatcaata cacctaacgg cgttctggtt gccagtggta
      481 aacatgaaga tatgtacact gcaattaacg aattgatcaa caagctggaa cggcagctca
      541 ataaactgca gcacaaaggc gaagcacgtc gtgccgcaac atcggtgaaa gacgccaact
      601 tcgtcgaaga agttgaagaa gagtagtcct ttatattgag tgtatcgcca acgcgccttc
      661 gggcgcgttt tttgttgaca gcgtgaaaac agtacgggta ctgtactaaa gtcacttaag
      721 gaaacaaaca tgaaacacac accgtttttc ttcgcattct tttttacctt cccctgaatg
      781 ggaggcgttt cgtcgtgtga aacagaatgc gaagacgaac aataaggcct cccaaaccgg
      841 ggggcctttt ttattgataa caaaaaaggc aacactatga catcggaaaa tccattactg
      901 gcgctgcgag agaaaatcag cgcgctggat gaaaaattat tagcattact ggcagagcgg
      961 cgcgaactgg ccgtcgaggt gggaaaagcc aaactgctct cgcatcgccc ggtacgagat
     1021 attgatcgtg aacgcgattt actggaaaga ttaattacgc tcggtaaagc gcaccatctg
     1081 gacgcccatt acattactcg cctgttccag ctcatcattg aagattccgt attaactcag
     1141 caggctttgc tccagcaaca tctcaataaa attaatccgc actcagcacg catcgctttt
     1201 ctcggcccca aaggttctta ttcccatctt gcggcgcgcc agtatgctgc ccgtcacttt
     1261 gagcaattca ttgaaagtgg ctgcgccaaa tttgccgata tttttaatca ggtggaaacc
     1321 ggcctggccg actatgccgt cgtaccgatt gaaaatacca gctccggtgc cataaacgac
     1381 gtttacgatc tgctgcaaca taccagcttg tcgattgttg gcgagatgac gttaactatc
     1441 gaccattgtt tgttggtctc cggcacgact gatttatcca ccattaatac ggtctacagc
     1501 catccgcagc cattccagca atgcagcaaa ttccttaatc gttatcctca ctggaagatt
     1561 gaatataccg aaagtacgtc tgcggcaatg gaaaaggttg cacaggcaaa atcaccgcat
     1621 gttgctgcgt tgggaagcga agctggcggc actttgtacg gtttgcaggt actggagcgt
     1681 attgaagcga atcagcaaca aaacttcacc cgatttgtgg tgttggcacg taaagccatt
     1741 aacgtgtctg accaggttcc ggcgaaaacg acgttgttaa tggcgaccgg gcaacaagcc
     1801 ggtgcgctgg ttgaagcgtt gctggtgctg cgcaaccaca atctgattat gacccgtctg
     1861 gaatcacgcc cgattcacgg taatccatgg gaagagatgt tttatctgga tattcaggcc
     1921 aatcttgaat cagcggaaat gcaaaaagca ttgaaagagt taggtgaaat tacccgttca
     1981 atgaaggtat tgggctgtta cccaagtgag aacgtagtgc ctgttgatcc aacctgatga
     2041 aaaggtgccg gatgatgtga atcatccggc actggattat tactggcggt tgtcattcgc
     2101 ctgacgcaat aacacgcggc tttcactctg aaaacgctgt gcgtaatcgc cgaaccagtg
     2161 ctccaccttg cggaaactgt caataaacgc ctgcttatcg ccctgcttca gcaactcaat
     2221 cgcctcgccg aaacgcttat agtaacgttt gattaacgcc agattacgct ctgacgacat
     2281 aataatgtcg gcataaagct gcggatcctg agcaaacagt cgcccgacca tcgccagctc
     2341 aaggcggtaa atcggcgaag agagcgccag aagttgctca agctgaacat tttcttctgc
     2401 cagatgcagc ccataagcaa aagtagcaaa gtggcgcaga gcctgaataa acgccatatt
     2461 ctgatcgtgc tcgacagcgc taatacgatg cagccgagcg ccccagacct gaatttgctc
     2521 cagaaaccat tggtatgctt ccggcttacg tccatcacac cagaccacaa cttgctttgc
     2581 caggctaccg ctgtccgggc cgaacatcgg gtgtaacccc agtaccgggc catcgtgcgc
     2641 cgccagcatg gcctgtaatg gtccattttt cactgatgcc agatcaacca gaatacaatc
     2701 tttcggtaaa ggcggtaatt tgccgataac ttgctcagta acgtggattg gcacactaac
     2761 aatcaccatt ccggcatcgg caacaatatc agccgctcga tcccagtcat gttgctccag
     2821 aatccgcacc tgataacccg agagtgtcag catcttctcg aacaggcgtc ccatctgacc
     2881 gccaccgccg acgataacca ccggacgcag tgacggacaa agtgttttaa atcctttgtc
     2941 gttttcactg gagtaagatt cacgcatcac ccgacgcaaa acatcctcaa tcagatctgg
     3001 cggtaccccc agagcttccg cctctgcacg acgcgaggcc aacatagatg cctcgcgctc
     3061 cggaacataa ataggcagtc caaagcggct tttcacctcg cccacttcag caaccagttc
     3121 cagacgcttc gctaataaat tcagcagcgc tttatcgact tcatcaattt gatcgcgtaa
     3181 tgcggtcaat tcagcaacca taataaacct cttaagccac gcgagccgtc agctgcccgt
     3241 tcagatcctg atgaatttca cgcagcaagg catcggtcat ttcccagcta atgcaggcat
     3301 cggttacgga tacaccgtat ttcatttcac tgcgcggttg ctcagaagac tgattgccct
     3361 cgtggatatt actttcgatc atcagaccaa taattgagcg attgccatct ttgatttgag
     3421 caaccacgga ttctgccacc gcaggctgac ggcgataatc tttattggaa ttaccgtggc
     3481 tgcaatctac catcagagac gggcgcagtc ccgcctgttc catctctttt tcacattgcg
     3541 caacatccgc agggctatag ttcggcgctt taccaccgcg caggatgaca tgcccgtccg
     3601 gattcccctg agtttgtagc aacgcaacct gccctgcctg gttaatgcca acaaaacggt
     3661 gcggctgggc ggcggcgcgc atagcgttaa ttgctgttgc cagactgccg tcggtgccgt
     3721 ttttaaaacc aaccggcatg gaaagcccgg aggccatttc acggtgagtt tgcgattccg
     3781 ttgtacgagc accaattgct gaccagctaa acagatcgcc caggtattgc gggctattcg
     3841 gatctaacgc ttccgtcgcc agtggcagtc ccatattcac cagctcaagc agcaatttac
     3901 gcgcgatctg cagcccggct tctacatcaa aagagccatc catatgggga tcgttaatta
     3961 accctttcca gccgacagtg gtacggggtt tttcaaaata gacgcgcatt accagataga
     4021 ggctatcgct gacctctgcg gcaagggctt taaatcgacg agcatattcc agagcagttt
     4081 ccagatcatg aatggaacaa ggaccacaaa ctaccagcag acgaggatcg cgcccggcga
     4141 taatatctga aatggtttta cgcgagtcag caatctgggc ttcttgttgc aggctcaatg
     4201 gaaaagcggc cttcagttgt tccggagtca ttaaaacctg ttcgtcggta atatgtacgt
     4261 tattcagcgc gtctttttgc atgatggcga tcctgtttat gctcgtttgc gatagttgat
     4321 cctcagcgag gatgacgtaa cgataacaca taaagtaaag ttttcaatcc atatttcgta
     4381 cgcttttctt tacacgaaca atttactcgc gctttgatgc ttatttcgta caaataaatt
     4441 tacaccccct ttaaaaacaa tccgctatgc tttgaaaaag gagaaagaaa tgatgaaaaa
     4501 gtttatcgcc cccttattgg ctttactggt tagcggatgt cagattgatc cttatactca
     4561 cgcgccaacc ttgaccagca ccgactggta tgatgtcggt atggaagatg cgatatcggg
     4621 cagcgccata aaagatgacg atgcatttgg cgattcacag gcggatcgcg gtctatacct
     4681 taaaggatat gccgaaggac aaaagaaaac ttgccagacc gattttactt atgcccgggg
     4741 actttccggt aaaagctttc ctgccagctg taacaatgtt gaaagtgcca gccaactgca
     4801 tgaagtatgg caaaaagggg ctgatgaaaa tgccagcaca atacgattaa attaattaaa
     4861 accccacaaa taaattaaac ataagatttt actcattgtg ttttatttct cacattgatg
     4921 acgatcgcat gaaaaaatga tattttaatg agcattctga aaaaatatcg atgcatttcg
     4981 agcgaagaag gtggggatcc ctgaatgcgt ttttctcacc gactgttctt acttctgatt
     5041 ctcctgctga cgggagcacc aatactcgct caggagcctt ctgacgttgc gaaaaacgtg
     5101 cgtatgatgg tttccggtat tgtcagttac acccgctggc ctgcgctatc agggccacca
     5161 aagttatgta tcttttcatc ttcacgtttt agtaccgccc tacaagagaa tgccgcgaca
     5221 tctttgcctt atctgcctgt catcattcac actcaacaag aggcgatgat ttcaggctgc
     5281 aatggttttt attttggcaa cgagtcaccg acattccaga tggaattaac cgaacaatat
     5341 ccgtcaaagg cgttattatt aattgccaaa caaaataccg agtgcattat tggtagcgcc
     5401 ttttgtctga tcatccacaa taatgacgtc agatttgccg taaacctgga tgccttatcg
     5461 cgtagcggtg taaaggtcaa cccggatgtc ttaatgctcg cacggaagaa aaatgatgga
     5521 taacgataat tctcttaata agcgccccac gtttaaaaga gcattacgca acatcagtat
     5581 gaccagcata tttatcacta tgatgctgat ctggttgctg ctttccgtga cctcggtgct
     5641 gaccctgaaa cagtacgcgc aaaaaaacct ggcactgaca gcagcaacaa tgacttacag
     5701 tctggaagca gctgtcgttt ttgccgatgg ccctgcagca actgaaacac tggcagcgct
     5761 gggccagcaa gggcaatttt caactgcaga agtacgtgat aagcagcaaa atattctggc
     5821 gtcctggcat tacacccgta aggatccagg cgatactttc agcaatttca taagccactg
     5881 gctcttcccc gcccccatca ttcagccgat tcgtcacaat ggtgaaacca ttggcgaagt
     5941 acgcttaacc gctcgcgaca gttcaatcag ccattttatc tggttttcgc tcgccgtact
     6001 gaccggttgt attctgctgg catcaggcat cgcaattacc ctcacccgcc atttgcacaa
     6061 tggcctagtg gaagcgctga aaaatatcac cgatgtcgta catgatgtgc gttccaaccg
     6121 caatttttcc cgacgagttt ctgaagaacg tatcgctgag tttcaccgct tcgctctcga
     6181 cttcaacagt ctgctggatg aaatggaaga gtggcagctt cgtttacagg ccaaaaatgc
     6241 gcagcttctg cgtaccgcgc tacatgaccc attaaccggg ctggctaacc gcgcagcgtt
     6301 tcgtagcggc atcaata
//
LOCUS       DAJTMC010000201         6291 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.201, whole genome shotgun sequence.
ACCESSION   DAJTMC010000201 DAJTMC010000000
VERSION     DAJTMC010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6291
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.201"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(83..619)
                     /gene="ssb1"
                     /locus_tag="OO130_003754"
     CDS             complement(83..619)
                     /gene="ssb1"
                     /locus_tag="OO130_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HCR5366988.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            873..3695
                     /gene="uvrA"
                     /locus_tag="OO130_003755"
     CDS             873..3695
                     /gene="uvrA"
                     /locus_tag="OO130_003755"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HCR5366989.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(3730..4086)
                     /locus_tag="OO130_003756"
     CDS             complement(3730..4086)
                     /locus_tag="OO130_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCR5366990.1"
                     /translation="MTISELLQYCMAKLGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLRPEEKRKLLVQL"
     gene            complement(4090..4506)
                     /locus_tag="OO130_003757"
     CDS             complement(4090..4506)
                     /locus_tag="OO130_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HCR5366991.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLANMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHDYEGADDMPSHIKSSMQGTSLVL
                     PLHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(4617..5330)
                     /gene="aphA"
                     /locus_tag="OO130_003758"
     CDS             complement(4617..5330)
                     /gene="aphA"
                     /locus_tag="OO130_003758"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HCR5366992.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            5732..5954
                     /locus_tag="OO130_003759"
                     /pseudo
     CDS             5732..5954
                     /locus_tag="OO130_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165333.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1591 a         1554 c         1568 g         1578 t
ORIGIN      
        1 taacggaggc ttctttttta tggctggcag atgctttaat catccacctt aaaacaatat
       61 aacctattgt tttaatgaca aatcagaacg gaatgtcatc atcaaagtcc atcggcggct
      121 cgttagacgg cgctgccgga gcggactgct gcgggcgaga ctgcgcgccg ccgctgaact
      181 gattgccacc ctgcggctgc tgaggctgac cccaaccgcc ctgcggctga ccaccaccga
      241 tattaccacc tgccggagcg ccaccaccct gacgaccacc cagcatctgc atggtgccgc
      301 caacgttcac cacgacttct gtggtgtagc gatcctgacc ggattgatcg gtccatttac
      361 gggtacgcag ctgaccttcg atataaacct gagaaccttt acgcagatat tcgctggcca
      421 cttctgccag tttgccgaac agcacaacgc ggtgccattc agtctgttct ttcatctcgc
      481 cggtcgcttt atcacgccag gattcggaag tagccagcgt aatgttggca actgcgccac
      541 catttggcat gtagcgtact tccgggtcct gacccagatt accaacgaga ataaccttgt
      601 ttacgcctct gctggccatg ttcgtgtctc ctgaaaaaaa tcgttctgaa taagtgtaaa
      661 cgcgcgattg taccattacc aatagcgctt ttactatgtt gtgacctcgg ttccgggaaa
      721 caaacctggc cagacattgt tacacaacac tccggataat gcattccaat actgtatatt
      781 cattcaggtc aatttgtgtc ataattaacc gtctgtgatc gccggtagca ccatgccacc
      841 gggcaaaaaa gcgtttaatc cgggaaaggt gaatggataa gatcgaagtt cggggcgccc
      901 gcacccataa tctcaaaaac atcaacctcg ttatcccccg cgacaagctc attgtcgtga
      961 ccgggctttc gggttctggc aaatcctcgc tcgctttcga caccttatat gccgaagggc
     1021 agcgccgtta cgttgaatcc ctttccgcct acgcgcggca gtttctgtca ctgatggaaa
     1081 agccggacgt cgatcatatt gaggggcttt ctcctgccat ctcaattgag cagaaatcga
     1141 cgtctcataa cccgcgttct acggtgggga caatcaccga aatccacgac tatttgcgtt
     1201 tgttgttcgc ccgcgtcggc gaaccgcgct gtccggacca cgacgtcccg ctggcggcgc
     1261 aaaccgtcag ccagatggtg gataacgtgc tgtcgcagcc ggaaggcaag cgtctgatgc
     1321 tactcgcgcc aatcattaaa gaacgcaaag gcgaacacac caaaacgctg gagaacctgg
     1381 caagccaggg ttacatccgt gctcgtattg atggcgaagt ctgcgatctt tccgatccgc
     1441 cgaaactgga actgcaaaag aaacacacta ttgaagtggt ggttgatcgc ttcaaggtgc
     1501 gtgacgatct tacccaacgt cttgcggagt cgtttgaaac cgcgctggag ctttccggtg
     1561 gtacagcggt agtggcggat atggacgacc cgaaagcgga agagctgctg ttctccgcca
     1621 acttcgcctg cccaatttgc ggctacagta tgcgtgaact ggagccacga ctgttttcgt
     1681 ttaacaaccc ggcaggtgcc tgcccaacct gtgacggcct tggcgtacag caatatttcg
     1741 atcctgaccg cgtgatccaa aaccccgagc tgtcactggc tggcggtgcg atccgtggct
     1801 gggatcgccg caacttctat tacttccaga tgctgaaatc gctggcagat cactataagt
     1861 tcgacgtcga agcgccgtgg ggcagcctga gcgcgaacgt gcataaagtg gtgttgtacg
     1921 gttctggcaa agaaaacatt gaattcaaat acatgaacga tcgtggcgat acctccatcc
     1981 gtcgtcatcc gttcgaaggc gtgctgcaca atatggagcg ccgttataaa gagacagaat
     2041 ccagtgcggt acgtgaagaa ttagccaagt ttatcagcaa tcgcccatgc gccagctgcg
     2101 aagggacgcg tctgcgtcgg gaagcgcgcc acgtttatgt cgagaatacg ccgctgcctg
     2161 ctatctccga catgagcatc ggtcatgcga tggaattctt caacaatctc aaactagctg
     2221 gtcagcgggc gaagattgca gaaaaaatcc ttaaagagat cggcgatcgt ctgaaattcc
     2281 tcgttaacgt cggcctgaat tacctgacac tttcccgctc ggcagaaaca ctttccggcg
     2341 gtgaagccca gcgtatccgt ctggcgagcc agattggtgc gggcctggtt ggcgttatgt
     2401 atgtactgga cgagccgtct atcggcctgc accagcgcga taacgagcgc ctgttgggta
     2461 cgcttatcca tctgcgcgat ctcggtaata ccgtgattgt ggtggagcat gacgaagacg
     2521 caattcgcgc cgctgaccat gtgatcgata tcggtcctgg tgcgggtgtt cacggcggtg
     2581 aagtggtcgc agaaggtccg ctggaagcga tcatggcggt gcctgaatcg ttgaccgggc
     2641 agtacatgag cggtaaacgc aagattgaag tgccgaagaa acgcgttccg gcgaatccag
     2701 aaaaagtgct gaagctgaca ggtgcacgcg gcaacaacct gaaggacgtg acgctcacgc
     2761 tgccagtcgg tctgtttacc tgcatcacag gggtttcagg ttccggtaaa tcgacgctga
     2821 ttaacgacac actgttcccg attgcccaac gccagttgaa tggtgcgacc atcgccgaac
     2881 cggcaccgta tcgcgatatt caggggctgg agcatttcga taaagtgatc gatatcgacc
     2941 aaagcccaat tggtcgtact ccacgttcta acccggcgac ctataccggc gtgtttacac
     3001 ctgtgcgcga actgtttgcg ggcgtaccgg aatcccgtgc gcgtggttat acgccaggac
     3061 gtttcagctt taacgtccgt ggcgggcgct gcgaagcctg tcagggcgac ggtgtgatca
     3121 aagtggagat gcacttcctg ccggatatct acgtgccgtg cgatcagtgc aaaggtaaac
     3181 gctataaccg tgaaacgctg gaaattaagt acaaaggcaa aaccatccac gaagtgctgg
     3241 atatgaccat cgaagaggcg cgtgagttct ttgatgcggt gccagctctg gcgcgtaagc
     3301 tgcaaacgtt gatggacgtt ggtctgacgt acattcgcct ggggcagtcc gcaaccacac
     3361 tttctggtgg tgaagcccag cgcgtgaagc tggcgcgtga gctgtcaaaa cgcggcaccg
     3421 ggcagacgct gtatattctc gacgagccga ccaccggtct gcacttcgcc gatattcagc
     3481 aactcctcga cgtgctgcat aaactgcgcg atcagggcaa taccattgtg gtgattgagc
     3541 acaatctcga cgtgatcaaa accgctgact ggattgtcga cctgggacca gaaggcggca
     3601 gtggcggcgg cgaaatcctc gtctccggta cgccagaaac cgtcgcggag tgcgaagctt
     3661 cgcatacggc acgcttcctc aagccgatgc tgtaatcgtt aaggccgctt tctgagcggc
     3721 cttttccttt cagagttgta ccagcaattt acgcttttct tccggtcgta aattcactgc
     3781 ctgctgataa gacgcatcca ccagataata gatttgcgaa tccggcagcg aaccgtcgag
     3841 atacacggtg ctccagtgcg ctttattcag atggcggcta ggacgcacat cgctgtgctg
     3901 ctgacgtagc agctccgcca gctccgggct ggttttcagc gaaacagctg ggcgattttc
     3961 tacttctttc accatcgcaa acagtacatc ttccactttg atctgcgtcg ctttccagtc
     4021 gttatgcacg ctctgttctg cgcctagttt tgccatgcaa tattgtagca actccgaaat
     4081 ggtcattttt tactcccctt gtagtgtcgc gatgatgcga cgcgatccgc cgtggatgcg
     4141 atgttccccc agccagatgc cttgccaggt gccggtctga atacgccctt tatgtaacgg
     4201 caatacaagc gatgttccct gcattgagga tttgatatga gaaggcatat cgtctgctcc
     4261 ctcatagtca tgctcataat ttccgttgtc gggaacggtg cggaggaaaa aacgctccat
     4321 gtcgtggcgt acggtgggat cgcagttctc attaagtgtc agagaggcgg aggtatgttg
     4381 cagcaacaga tgcagtaagc cgatgttaac gcgcggcata ttagccagct gattcagaat
     4441 ttcatccgtt accagatgaa acccacgaga tttggcgcta agcgtgagcg tcttttgata
     4501 ccacatgtgc tgctccttga taaaactctc ttaatcagtt tgcagcaaga cagcgaaagg
     4561 ataaaggtgt gattaaaaaa acagcattcg ggagagcatc acgctctccc gctctgtcag
     4621 tattctgaat tgacgatcac ctcttcacca aacgcacccg cttgtggcaa gggtttgtag
     4681 gtagagttgg aggcgcgcag aatgcggata ccacgagcgc cgacatcgcg tgcggcggta
     4741 atatcattat cagaatcgcc ataaaaaatt cggatatttt tatcctgcag ccattgcgat
     4801 tttgtatttt gccctggttt atcgcccgca aagatcaccg gattcatgtt ggtggcagga
     4861 atatgaaaat tatccgccag cgtttttgaa accgtttctg ttttcgtcgg actacgacca
     4921 gtcacaaaga agatcgcgtc accgcggcgt acatgcatat caatcagctg gcgagcgacc
     4981 tcttttggaa tgctgaattc atcccagcca ttgttcattt tttcccagaa cacaggattt
     5041 ttcagataat cttcgctttc tggcgagaag gtttttttgc cgcgccagaa gcccgggctg
     5101 gaaaaaagta ccgtgtcatc gatatcaaac cccaccgcca ttggcggacg ccctgcgagg
     5161 ctattttcaa tttgtgcgac cgaaacccaa tgaattggtg cctgttcagc aagcctggca
     5221 acgttagtac cagggttaag cggtgaagga gatgaggcca gggcaacagc ggaactgttt
     5281 agagcgaaca ataagcaaac ggcactgatt gcctgtgtga tcttgcgcat atttttccct
     5341 aaatagtcag tatgttgaaa ctttttattg tgagatttgt tgcaaaaacc atctgaccat
     5401 aacgccagct gcaggggaaa ggaaggatta tctgcggttt ttgtgatgaa aagcaggatg
     5461 attatattta acccagtaaa tacgttcctg tctttttttg tataaaaaat gtttattact
     5521 cgatgtaagg atacattaat tttacgttta ttatgatgaa tcctctgcaa atgagcgaaa
     5581 tttgatagaa catttatctg ctgtacgatt taatgaactt aatgatacag cattacaaaa
     5641 acaatcgaag tttataaaga tgattcctga ttgaccagaa ccctatccta caatggtgat
     5701 taattttatt actcgcgatg gagatgatga tatgaatata tcatatatga atagcaacaa
     5761 aacgacatct ttacctgttg agctagatgc actaaataat aaagatatct cttatgccaa
     5821 agatttttct tatgtcaaag atttcttttt atacatagaa acactgttaa aaattgcaaa
     5881 agatttttgt agacttggag aagaagtatc aagttctatt gcaagtaaag tttttcacgc
     5941 atttattgat ttagttaaca aaatcagggg taagaaagac tttatgtata tttgcacgct
     6001 ttgctgtttt gccgaggagg ttaaaggtga ttattctcat tacaggacct ttttatttga
     6061 tattggtaat caatataagg ttaaacttac acaaagcgga aaaaaagagt tctctttaac
     6121 attagaattt aacgatacta taattgaatc tcagaaagtc acaggcaata aggcaaagca
     6181 tattcttgag gatatagaaa tattctatcg taacaaaccc gatacttatt attaaattta
     6241 ataatggtta gatgtgcaga aattattact ccacacgaaa aaatagggaa a
//
LOCUS       DAJTMC010000202         6146 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.202, whole genome shotgun sequence.
ACCESSION   DAJTMC010000202 DAJTMC010000000
VERSION     DAJTMC010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6146
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.202"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(55..180)
                     /locus_tag="OO130_003760"
                     /pseudo
     CDS             complement(55..180)
                     /locus_tag="OO130_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="receptor for colicin S4; incomplete; partial in the
                     middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
     gene            366..641
                     /locus_tag="OO130_003761"
     CDS             366..641
                     /locus_tag="OO130_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HCR5366993.1"
                     /translation="MTVFVLRGYIPHTTAKSVVGIGVSDNITTHSRVCGFFCVKHCHI
                     RIMVGRAGAEKSAPGFVMTGYANPVRLTTSLIGVRGGDHPINWSHHI"
     gene            638..1195
                     /locus_tag="OO130_003762"
     CDS             638..1195
                     /locus_tag="OO130_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCR5366994.1"
                     /translation="MNTAITFSNESPKVFFKDGRLVTTSQAVADYFDKQHKHVLAKID
                     TLDCSPEFTSANFSADVQTVEIGNGAERESRCYLITKDGFMFLVMGFTGKKAARLKEA
                     YIEKFNAMEEELHKRPPAAQNSPAPNNGCALLIHFDKHGQVEFTEKVPADAMVCTLER
                     FKFYLEQHGWIVARKEQLVERLMRF"
     gene            complement(1322..1492)
                     /locus_tag="OO130_003763"
     CDS             complement(1322..1492)
                     /locus_tag="OO130_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001710241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5366995.1"
                     /translation="MVRLPPVVMSAKSATARTTIAREDAEDHQVKLSAQKLEELLASM
                     VKDEVDRNDGIY"
     gene            complement(1607..1876)
                     /locus_tag="OO130_003764"
     CDS             complement(1607..1876)
                     /locus_tag="OO130_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR5366996.1"
                     /translation="MLPQHSDIEIAWYASIQQEPNGWKTVTTQFYIQEFSEYIAPLQD
                     AVDLEIATEEERSLLEAWNKYRVLLNRVDTSTAPDIEWPANPVRE"
     gene            1945..2601
                     /locus_tag="OO130_003765"
     CDS             1945..2601
                     /locus_tag="OO130_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000742376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR5366997.1"
                     /translation="MKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEHLLPH
                     FLEHVGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHKISHD
                     VFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATILGDG
                     VVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSASGKK
                     "
     gene            complement(2656..3078)
                     /locus_tag="OO130_003766"
     CDS             complement(2656..3078)
                     /locus_tag="OO130_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR5366998.1"
                     /translation="MAVFNSDEASWHLVEDHRGKTVYDVASGDALFISELGPLPENVT
                     WLSPEGEFQKWNGTAWVKDAEAEKLFRIREAEETKNSLMQVASEHIAPLQDAVDLEIA
                     TEEETSLLEAWKKYRVLLNRVDTSTAPDIEWPTNPVRE"
     gene            complement(3251..3923)
                     /locus_tag="OO130_003767"
                     /pseudo
     CDS             complement(3251..3923)
                     /locus_tag="OO130_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003910815.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
     gene            complement(4602..4781)
                     /locus_tag="OO130_003768"
                     /pseudo
     CDS             complement(4602..4781)
                     /locus_tag="OO130_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003910815.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
     gene            complement(4758..4910)
                     /locus_tag="OO130_003769"
                     /pseudo
     CDS             complement(4758..4910)
                     /locus_tag="OO130_003769"
                     /inference="COORDINATES: protein motif:HMM:NF015371.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
     gene            complement(4859..5071)
                     /locus_tag="OO130_003770"
     CDS             complement(4859..5071)
                     /locus_tag="OO130_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5366999.1"
                     /translation="MRRPDRRQQRRVAPRRHPRRRQRQLAVRRRQLRAKVRRNLQQRA
                     PRQRQNGQRILHPPWRLRMRARRKRG"
     gene            5029..5766
                     /locus_tag="OO130_003771"
     CDS             5029..5766
                     /locus_tag="OO130_003771"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5367000.1"
                     /translation="MALPFAADACLVASSFEADADEADDDAELADDAAAVFEDSALVS
                     DAFAFVSDVFAAEADLAAALACSVASPAFVVAVEADDAALSADFPAAVALAEACPALV
                     DAALADDAAAVFEPAAAEALCPAAVSEDLAFVSDVFAAFAEFPAAVAEEAALLALEDA
                     FVSDDFAASFEAAASRAEVAASDAFVVAVDAEVAADCCDAAAFVSDVFAALALVAAAL
                     FEDSAAAALFDASVAFVDAVPALEVAD"
     gene            complement(5862..6146)
                     /locus_tag="OO130_003772"
                     /pseudo
     CDS             complement(5862..6146)
                     /locus_tag="OO130_003772"
                     /inference="COORDINATES: protein motif:HMM:NF019996.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
BASE COUNT         1404 a         1591 c         1470 g         1681 t
ORIGIN      
        1 tttatgccat gtccttattg accccgtata ttacggggtc gtttttgtgc ggaattaaaa
       61 acgatatcct gctgagaaca taaacaccca cggatcgagg cgtacgctat catgttgctg
      121 tgcaccgccc agcttataat tggcggtggt atcgatatcc atgtaccaga ctgacatgtt
      181 ttaaaaaatg caataagaca ttgtatctga aagaaaaaac aggattttga ggcgcttttt
      241 tataccgttt ttccgtgttt ttaactgcct gattttattg aaatcttttt tagttttgca
      301 gaaaggattt tttgctcgat cgctttaatt ttatacattc ctgataacgg aaattttttt
      361 gcccgttgac agtttttgtt ctccggggct atattcctca cacaaccgca aaatcggttg
      421 tcgggattgg cgtctcggat aacatcacaa cgcatagccg cgtttgcggt tttttttgcg
      481 ttaagcattg ccacattcgc attatggtgg ggcgtgcagg ggcggagaaa tccgcgccgg
      541 gttttgtgat gaccggttac gccaaccctg tacgtctcac cacctccctg attggcgtca
      601 gaggtggtga tcatccaatt aattggagtc atcacatatg aatacagcta taactttttc
      661 taacgagtca ccaaaagtct ttttcaaaga tggtcgcctg gttaccacct ctcaagctgt
      721 tgccgactac ttcgataaac aacataaaca cgttttagct aagattgaca cactcgactg
      781 ctctcccgaa tttacatcag ccaacttttc ggccgatgtt cagactgtcg aaattggcaa
      841 cggcgcagaa cgtgaatcac gatgctacct gatcacaaaa gacggcttca tgttcctcgt
      901 aatgggattt actggcaaaa aagcagccag actaaaagaa gcttacattg aaaaatttaa
      961 cgcaatggaa gaagaactcc acaaacgtcc tcctgcagca caaaactccc ccgccccaaa
     1021 taatggatgc gcattactga tccacttcga taaacacggt caggtcgagt tcacagaaaa
     1081 agtccccgcc gatgcgatgg tatgcacgct ggaacggttt aaattttatc tggagcaaca
     1141 tgggtggatc gttgcccgta aagaacaatt ggtggaaagg ttgatgcggt tttaatgaat
     1201 gcaaaacccc ggatgatcac cggggttaat tttatcgagc gtgattgccg ctaatcgcca
     1261 tcgctctgat tgagcgtaaa tcattcaggt tattcagttc ttccttctgc tctcgctgac
     1321 gtcaataaat cccatcattg cgatcaacct catccttaac cattgatgcg agcagttctt
     1381 ccagtttctg cgctgacagc tttacctggt gatcttcagc atcttcccgg gctattgtgg
     1441 ttcgcgcagt ggctgatttt gctgacatca ccacaggggg cagacggacc attgaaccat
     1501 ggcctccgga acaaataagg gcaaaaataa caaccactat cgaaagctca caaactaacc
     1561 gcagcacgtt cctgcatacg acgtgtctgc ggcataatcc caatgattac tccctgacag
     1621 gatttgcagg ccactcaata tcaggtgcag ttgatgtatc aacacgattc aacaataccc
     1681 gatatttatt ccatgcctcc agcaacgatc tttcttcctc cgttgcgatt tccagatcta
     1741 cagcatcctg cagtggcgca atatactcac tgaattcctg gatgtagaac tgtgtggtga
     1801 cggtcttcca gccattcggc tcctgctgta tcgaagcata ccaggctatt tcaatatcgc
     1861 tatgctgcgg cagcatttaa ccccttgtaa ttcatcgcca taattgattt aattcacaaa
     1921 taaaactata acacggtgaa atcaatgaaa aaaaacacag atgatggggc taaaatttac
     1981 acaccactta ccctaaagct ttatgactgg tgggttttgg gagtatcaaa tcggcttgca
     2041 tggggatgtc ctacaaagga acaccttctt ccacactttc tggaacatgt aggtaacaac
     2101 catctggata ttggtgttgg aactgggttt taccttactc acgtacctga gagtagtctg
     2161 atatctttaa tggatttgaa cgaagctagc ctgaacgcgg catctacaag ggctggggaa
     2221 tcaaaaatta aacataaaat tagccatgat gtttttgaac cttatcccgc ggcgttacat
     2281 ggtcaatttg attccatttc catgttttac cttcttcact gcctgcctgg aaatatatct
     2341 acaaaaagct gtgtaatacg caatgcggcg caggccttaa ctgacgatgg aactctatac
     2401 ggagccacaa ttcttggcga tggagttgtg cacaatagct tcggtcaaaa actgatgcgc
     2461 atttacaatc agaaaggcat cttttcaaac acaaaagatt ccgaagaagg cttaacacat
     2521 atactctcag agcatttcga gaatgttaaa accaaggttc aaggtactgt agtaatgttt
     2581 tccgcttcag ggaaaaaata gcatccaacc gcagcacgtt cttgcttaag acgtgctgcg
     2641 gcataatccc aatgattact ccctgacagg gttcgtaggc cactcaatat caggtgcagt
     2701 tgatgtatca acacggttca gcaacacccg atattttttc caggcttcca gcaatgaggt
     2761 ttcttcctcc gttgcgattt ccagatctac agcatcctga agtggcgcaa tatgctcact
     2821 ggctacctgc atcaggctgt tttttgtttc ttccgcctcc cggatccgga acagtttttc
     2881 tgcttctgca tctttcaccc aggctgtgcc gttccacttc tgaaactccc cttccggcga
     2941 taaccaggta acattttccg gtaacggacc gagttcagaa ataaataacg cgtcccctga
     3001 cgctacgtca taaaccgttt taccccgatg gtcttcaacg agatgccacg atgcctcatc
     3061 actgttgaaa acagccacga agcctgccgg aatatctggc ggtgcaatat cagtactgtt
     3121 tgctggcaga cctgtatgag gcggaatata tgcatcacct tcaccaataa attcattagt
     3181 tccggccagc agattataaa tttttatggt ccgtggttgt tcactcattc tgaatgccat
     3241 tatgcaagcc tcacaatata gttaaatgcg atgtttttga cggtgttttc cgcgttacca
     3301 gcagcgttaa cggtgatggt atgtccatgt gaaccaatcg caacggagtg cgtatgagca
     3361 ccaataccga cagtatgtgc gtgtgcacct gcgcttgcag cagtacccga tacagagtgc
     3421 gtatgagcgc ctgcagaaga agtgttaaca ctgtatttag aataatcagg tgatccactt
     3481 cccggagcac cactggaacc acctgaaaca aatgtcatcg aatgggtatg tgcaccggct
     3541 gaagcagccg taccgctaac actatgggta tgcgcaccag tgttattcgt ggatttagtg
     3601 ccgtaatcaa acgacgatgt ggttttcgtc cccaaatccg tactggatac gctggcgctg
     3661 tgggtgtgtg atttaatgcc gtcctgttcc tgagacaata cggctcgatc actggcgggc
     3721 ttgcccttaa tcgtccagcc acgcatatca gggatcacgc ctgacggata agcggctgca
     3781 agtttcgggt aagcagattt gtcaaaagtc tgcccctgca tcagggcata accagacgga
     3841 acggtatctg atggccacgg gattggtgca ccgactggat aaaactctgc aggaggatga
     3901 gccgaggtgt aaagctgcgc ccacggcgac cagtttgcgt cggtcgtatc ccgtcgtgaa
     3961 cgaataaatg ccggagcatg agcaccgctt gtaccactcc agccgatgag taactcacct
     4021 tcgccaacgg ctgtcatccc tttcaggtga atgatatttc catacgctgt tggatatccg
     4081 ttgttataca cctcgtataa ctcaagacct gttgccccct gcgtattgtc tgtcagcgcg
     4141 gttacccgac cttttgaaga cagattaact gatgatactg ctgttccacc tgacggtaac
     4201 gccccgatct ctgatgccgt tggcttattt ctggagttat agtcccttcg ccagccaggt
     4261 gaataatctg ttccgtgatt aatataggta aactgggcgt tagttgttcc accaccagtg
     4321 gaggtggtcg gtgtggtaat gtggatcgtc atcgctgact ttatccccat tacttcaatg
     4381 acagctccgg cgagatgaat attaccgcag ccagtatcag taatgatttt attattgcca
     4441 taagaccagg aacccttgca catccagtat ggatggttaa atgctccctg agaatccagc
     4501 cactcgataa actgtgcagt cgtccagttt cctgttgttg tgcttactga cccaccgaag
     4561 gcacggcagg caccaatatt tttcgtaaag gtgtctttgc cagggatatc cgcaccgttc
     4621 tgatctttct gcagacgttt ctcagcattg tcattggctg cttttactgc ctttggcgtt
     4681 gccgccagcg tttcagacgt gctgttggtc gcgctgctta gctggattat ccctttctgt
     4741 gctgtcgttg catcctgtgc ggtgtatttc ccgttagcca ggtcatacgc ggccttaacg
     4801 gcttttggcg ttgccgccag tgactcggaa gtgctgttgg tcgcactgct gagctgtact
     4861 atcccctttt tcgtcgtgct cgcatcctca agcgccacgg cggatgcaat atcctctgcc
     4921 cgttttgccg ctgtctcggc gcgcgttgct gcagattccg ccgtactttt gctctgagct
     4981 gccgccgtcg cactgccagc tgcctctgtc gccttcgtgg atgccgtcgt ggcgctaccc
     5041 ttcgctgctg acgcctgtct ggtcgcctca tcttttgaag cagacgccga tgaggccgat
     5101 gacgacgccg aactggctga cgatgcggca gccgtttttg aggattctgc gctggtttcc
     5161 gacgctttcg cgttcgtttc ggatgtcttc gctgcggaag cagacctcgc tgctgcgctg
     5221 gcctgttcag tggcttcgcc agccttcgtt gtggctgttg aagcagacga tgcggcgctt
     5281 tctgccgatt ttccggcggc ggtggcactg gctgaggcct gcccggcact tgttgacgct
     5341 gcactggcag acgacgcagc cgctgttttt gagcctgccg cagccgaggc gctctgtccc
     5401 gctgccgttt cagaagacct ggcgttcgtc tctgacgttt ttgccgcctt cgcggaattt
     5461 cctgccgctg ttgccgagga agctgcgcta ctggcgctcg aggatgcgtt cgtttctgat
     5521 gattttgccg cctcttttga agccgccgca tcccgggctg aggtggcagc ttctgacgct
     5581 ttcgtagtcg cggtggatgc agaagtggct gctgattgtt gtgacgctgc agcattcgtt
     5641 tctgacgttt tcgcggcact ggcactggta gctgccgcgc tttttgagga ctctgcagcg
     5701 gcagcacttt ttgatgcttc agtggccttt gttgatgccg ttcctgcgct ggaagtcgct
     5761 gactgagccg acgacgcggc ctgtccggct gacgtgctgg ctgcgcgtgc tgagcctgca
     5821 gcatcagtcg catgggttgc cgcctcacgg gctgatgtgc tggcatcact ggctgacttc
     5881 ttcgcggctg ccgtgttctg tgccaccacg gacgcgttac gcgccacctc ttccaccatc
     5941 agctcaaaac ggcgcagtgc ctccggacgg gcatcatcct ccgtcatggc accgagaaaa
     6001 tcattcagcg taccgggtcg ggaatcttca tacacggtga tggtcccggc atgtgacggc
     6061 gggaatcctt ccaccaacag aataacgctg tactgaccgt actcaacgtc catgctgtaa
     6121 cgcccggctt catccggatt ttcaga
//
LOCUS       DAJTMC010000203         6106 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.203, whole genome shotgun sequence.
ACCESSION   DAJTMC010000203 DAJTMC010000000
VERSION     DAJTMC010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6106
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.203"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(<1..236)
                     /gene="yneK"
                     /locus_tag="OO130_003773"
     CDS             complement(<1..236)
                     /gene="yneK"
                     /locus_tag="OO130_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HCR5367001.1"
                     /translation="MVTPISISNYIFLPDDFPVRNIAPQVKEVLKNFIDALSTIICDE
                     EWRTSLNTNSATKKIFNNLDNLSYIQRTSFRGNDT"
     gene            complement(314..1195)
                     /locus_tag="OO130_003774"
     CDS             complement(314..1195)
                     /locus_tag="OO130_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5367002.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAIVLRVPSNLTIRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNHRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVIRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLDVPDSAKQGYQ"
     gene            1296..2684
                     /gene="sad"
                     /locus_tag="OO130_003775"
     CDS             1296..2684
                     /gene="sad"
                     /locus_tag="OO130_003775"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HCR5367003.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGANDIENALQLAAAGFRD
                     WRETNIDYRAEKLRGIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNDGVSQMIKDSRIAAVTVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMGDPRDEENALGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITVAKDAEHALELANDSEFG
                     LSATIFTTDETQARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRI"
     gene            2748..3674
                     /gene="glsB"
                     /locus_tag="OO130_003776"
     CDS             2748..3674
                     /gene="glsB"
                     /locus_tag="OO130_003776"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HCR5367004.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            3674..4033
                     /locus_tag="OO130_003777"
     CDS             3674..4033
                     /locus_tag="OO130_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HCR5367005.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVVQMNQP"
     gene            4172..5590
                     /locus_tag="OO130_003778"
     CDS             4172..5590
                     /locus_tag="OO130_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCR5367006.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIVAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINSLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            5817..6056
                     /locus_tag="OO130_003779"
                     /pseudo
     CDS             5817..6056
                     /locus_tag="OO130_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="catalyzes the formation of D-tagaturonate from
                     D-altronate; incomplete; partial in the middle of a
                     contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
BASE COUNT         1455 a         1405 c         1635 g         1611 t
ORIGIN      
        1 gtgtcgttac cccgaaaaga agtcctctga atatatgata agttatcaag gttgttaaat
       61 atcttttttg tggctgagtt ggtgtttaaa gacgtgcgcc actcttcatc acatattatg
      121 gtactgagtg catcaataaa attctttaaa acttctttta cctgaggtgc aatattacgt
      181 acgggaaaat catcaggaag aaatatgtaa ttactgatgc ttattggcgt aaccatattt
      241 gccccatata gtcataagtc atcggaacat cactatatag ggcatttgag ctgcaagaac
      301 tatcaaaaat agctcattgg taaccctgtt ttgccgagtc aggcacatcc agtagttgaa
      361 taaatgcctc aagcggacgc gttttcgcac cacgacgcca gaccagccag gttgttaacc
      421 aacgccattg ctcagctaac ggccacgctt caacctggtg atgccccggc atactttcca
      481 gcatagagcg tggaataagc gcaatgcctg ctcctgcgac cacacaggcc aacatcccgt
      541 gatacgactc catctcgtga atagttcccg gagcggcacc gtcagcatga aaccagctct
      601 cgaagtggcg acgatacgaa caattggcgc ggaaggcata aatgttactg ccgttaacct
      661 gactggcgcg gattactggc gcatgacctt gtggcgtgac gatcatcagc tcctcgcggt
      721 ataccggtat cccgtcaatg gcagtatggt taatgggccc atcaataaat gccgcattca
      781 gttttccctc cagcactcct tccagcatag tgccggaagg gccggtggaa agtgaaaact
      841 gaattttggg ataacgatgg ttgaattccg ccagcgtggc cggaatgcgc actgctgcgg
      901 tgctttccag cgaaccaaga gaaaataaac cttgcggctc atcgccagcg acaacgctcc
      961 gcgcttcatc cactaacgcg agaatttgtt ggctatagcg taaaaagtta tgcccggcag
     1021 gagagagacg taaacgctga ttctcgcgaa taaacagatc tacccccagt tctgtttcca
     1081 gctggcgtag acggatagtg agattcgacg gcacgcgaag cactattgct gctgcctggg
     1141 ttatgctgcc agcttcggca accgcgttga acatctccag ttgggtcaga tccataatat
     1201 tctcgattcg tgaataagtg gcttaatatt attcatttta aagcaagagt aaatctgcgt
     1261 atcttcatac catgactcat aaaggagata ccccgatgac cattactccg gcaactcatg
     1321 caatttcgat aaatcctgcc acgggtgaac aactttctgt gctgccgtgg gctggcgcta
     1381 acgatatcga aaacgcactt cagctggcgg cagcaggctt tcgcgactgg cgcgagacaa
     1441 atatagatta tcgtgctgaa aaactgcgtg gtatcggtaa ggctctgcgc gcccgtagcg
     1501 aagaaatggc gcaaatgatc acccgtgaaa tgggcaaacc aatcaatcag gcgcgcgctg
     1561 aagtggcgaa atcggcgaat ttgtgtgact ggtatgcaga acatggtccg gcaatgctga
     1621 aggcggaacc tacgctggtg gaaaatcagc aggcagttat tgagtatcga ccgttgggga
     1681 cgattctggc gattatgccg tggaactttc cgttatggca ggtgatgcgt ggcgcggttc
     1741 ccatcattct tgcaggtaac ggctacttac ttaaacatgc gccgaatgtg atgggctgtg
     1801 ctcagctcat tgcccaggtg tttaaagatg cgggaatccc gcaaggcgta tatggctggc
     1861 tgaatgccga caacgacggt gtcagtcaaa tgattaaaga ttcgcgcatt gctgctgtca
     1921 cggtgaccgg aagtgttcgt gcgggagcgg ctattggcgc acaggctgga gcggcactga
     1981 aaaaatgcgt actggaactg ggcggttcgg atccatttat tgtgcttaac gatgccgatc
     2041 tggaactggc ggttaaagcg gcggtagccg gacgttatca gaataccgga caggtttgtg
     2101 cagcggcaaa acgctttatt atcgaagagg gaattgcttc tgcatttacc gaacgttttg
     2161 tggcagctgc ggcagccttg aaaatgggcg atccccgtga tgaagagaac gctctcggac
     2221 caatggctcg ttttgattta cgtgatgagc tgcatcatca ggtggagaaa accctggcgc
     2281 agggtgcgcg tttgttactg ggcggggaaa agatggctgg ggcaggtaac tactatccgc
     2341 caacggttct ggcgaatgtt accccagaaa tgaccgcgtt tcgggaagaa atgtttggcc
     2401 ctgttgcggc aatcaccgtt gcgaaagatg cagaacatgc gctggaactg gctaatgata
     2461 gtgagttcgg cctttcagcg accattttta ccaccgacga aacacaggcc agacagatgg
     2521 cggcacgtct ggaatgcggt ggggtgttta tcaatggtta ttgtgccagc gacgcgcgag
     2581 tggcctttgg tggcgtgaaa aagagtggct ttggtcgtga gctttcccat ttcggcttac
     2641 acgaattctg taatatccag acggtgtgga aagaccggat ctgacgcgac atattcagct
     2701 ctgatatact cgcaggtctt ttcagacctg cggtccagga gtagaaagtg gcagtcgcca
     2761 tggataatgc aattttagaa aacatcttgc ggcaagtgcg gccgctcatt ggtcagggta
     2821 aagtcgcgga ttatattccg gcgctggcta cagtagacgg ttcccgattg ggcattgcta
     2881 tctgtaccgt tgacggacag ctttttcagg ccggagacgc gcaagaacgt ttttccattc
     2941 agtctatttc caaagtgctg agtctcgttg tcgccatgcg tcattactcc gaagaggaaa
     3001 tctggcaacg cgtcggcaaa gatccgtctg gatcaccgtt caattcttta gtgcaactgg
     3061 aaatggagca gggtattccg cgtaatccgt ttattaatgc tggtgcgctg gtggtctgcg
     3121 atatgttgca agggcgatta agcgcaccac ggcaacgtat gctggaagtc gtgcgcggct
     3181 taagcggtgt gtctgatatt tcatacgata cggtggtagc gcgttccgaa tttgaacatt
     3241 ccgcgcgaaa tgcggctatc gcctggctga tgaagtcgtt tggcaatttc catcatgacg
     3301 tgacaaccgt tctgcaaaac tactttcatt actgcgctct gaaaatgagc tgtgtagagc
     3361 tggcccggac gtttgtcttt ctggctaatc aggggaaagc tattcatatt gatgaacctg
     3421 tggtgacgcc aatgcaggcg cggcaaatta acgcgctgat ggcgaccagt ggtatgtacc
     3481 agaacgcggg ggagtttgcc tggcgggtgg ggctaccggc gaaatctggc gttggtggcg
     3541 gtattgtggc gattgttccg catgaaatgg ccatcgctgt ctggagtccg gaactggatg
     3601 atgcaggtaa ctcgcttgcg ggtatcgccg tccttgaaca attgacgaaa cagttagggc
     3661 gttcggttta ttaatgcagt ctctcgatcc actcttcgcg cgtttatccc gttcaaaatt
     3721 tcgctctcgc tttcgtctgg gcatgaaaga gcgtcagtat tgcctggaga aaggcgcgcc
     3781 agtcatcgaa caacatgcgg cggattttgt cgctaaacgt cttgctccgg cattaccggc
     3841 taatgacggt aagcaaacac ccatgcgcgg gcatccggtg tttatcgctc agcacgccac
     3901 cgcgacctgc tgtcgcggtt gtctggctaa gtggcataac attccgcagg gtgtttcgtt
     3961 aagcgaagaa caacaacgtt atatcgtggc ggtaatctat cactggctgg ttgtacagat
     4021 gaatcagcca tgagaattgc ggaaaacgat aaaatagcat tcccagggta cctttatttg
     4081 ccgccgctgt tagcgaacat gctatccttt atgagaattt acttattacg ttgagataat
     4141 tgccacccgc aattgtatgg acagataatt aatgcatgta caccccattt caactttccg
     4201 tttgttccag gaaggtcatc tgctacgtaa tagcatcgct atttttgcgc taaccacgct
     4261 gttctatttt attgttgcag agttacggct ggttcacgaa ctttctcttt tctggccgct
     4321 gaatggcgta atggcggggg tgtttgcccg ctatgtctgg cttaatcgac tgcattacta
     4381 tgcgatcagt tatgtggcga tgctggttta tgatgccata accaccgaat ggggactggt
     4441 ttcactggtt atcaatttct ccaatatgat gtttattgtt accgtcgcct tactggtcgt
     4501 gcgggataag cgtctgggga aaaataagta tgagccagta agtgccttac ggctatttaa
     4561 ttactgtctg attgccgcat tattatgcgc tattgtcggg gcgattggtt cggtcagtat
     4621 tgatagtctg gatttctggc ctttgcttgc ggactggttc agtgagcaat tctcaacggg
     4681 cgtgttgatc gtgccttgta tgctgacgtt ggctattcct ggagtgctgc cgcgctttaa
     4741 agcagaacag atgatgcccg ccatcgcgct tattgtgtcg gttattgcct cggtagtcat
     4801 tggcggagcg gggagtctgg cgttcccgct gcctgcatta atctggtgtg cagtgcgcta
     4861 tacgccgcag gtaacatgtc tgttgacctt tgtcaccggt gcggtggaaa tcgtactggt
     4921 ggcaaattcg gtgattgata tctcggtcgg ttcgccgttc tccattccgg aaatgttctc
     4981 cgcacgtctc ggtattgcca cgatggcgat atgcccaatt atggtttctt ttagcgtggc
     5041 agcgatcaat tcgctaatga agcaagttgc gctgcgagcc gactttgatt ttctgactca
     5101 ggtttactca cggtccggtc tttatgaggc gctgaaaagt ccatcgctga aacagacaca
     5161 acatctgacg gtcatgctgc ttgatatcga ctatttcaaa agcattaacg ataactatgg
     5221 acatgaatgt ggcgataaag tgttaagcgt gtttgcccgg catattcaga agattgtcgg
     5281 tgataagggg ctggtggcgc gcatgggcgg cgaagaattt gctgttgcag tgccgtcggt
     5341 gaatcctgtc gatggtctgc taatggcgga aaaaatccgt aaaggcgttg aactgcaacc
     5401 attcacctgg caacaaaaaa cgctttatct cacggtaagt attggcgtcg gtagtggtcg
     5461 cgcatcgtac cgaacgctga ccgatgattt taataaattg atggtcgaag ccgatacgtg
     5521 tctgtatcgc tcgaagaaag atgggcgcaa ccgtaccagc actatgcgtt acggtgagga
     5581 agttgtctga catctttatt cattccgcgc gatttgaatg caatgtgttc taccacttta
     5641 tgatccagga aaaggcgtat ttcctcgatt aaaccatgat ccgcgccaca cttttctcat
     5701 aaaactgctc attaatcatc aggagttgat caaaacaggg gttcggcgta ttaaattttg
     5761 ctaacctgtc gtaccaattc agacgggttg tatgactaat cagaagggaa cccattgtga
     5821 aaacactaaa tcgtcgcgat attcccggtg cacagtatcc agaacgtatc attcagtttg
     5881 gtgaaggtaa cttcctgcgc gcctttgttg actggcaaat cgatctcctg aatgagcaca
     5941 ccgatctgaa ttctggcgtg gtcgttgttc gtccgattga aacttcattc ccgccgtcac
     6001 tgagcacgca ggatggtctg tacaccacca ttatccgtgg cctgaatgag aaggggtgaa
     6061 gtggtcaaca aaaactggcc accgagttag agtttttcca gtatcg
//
LOCUS       DAJTMC010000204         6100 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.204, whole genome shotgun sequence.
ACCESSION   DAJTMC010000204 DAJTMC010000000
VERSION     DAJTMC010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6100
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.204"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(23..604)
                     /locus_tag="OO130_003780"
     CDS             complement(23..604)
                     /locus_tag="OO130_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5367007.1"
                     /translation="MYYPVTDYIALALIISFLFLTLFICLLCLKHERIKKETIRQKNA
                     HILEHGWNATEFSWFRYGQYNETGIYISIEKTIIITITVSGGCFKKEYSIVSHMLVTD
                     TITEATLYENGLYTRHIRLSRPVSDSKYPLPPGSQLIKNMTLRLRLQDQQEETSVTLF
                     QGKMSTDGNNYYIIKGKVSSVLLLLKMLQINHA"
     gene            complement(651..4508)
                     /gene="sigA"
                     /locus_tag="OO130_003781"
     CDS             complement(651..4508)
                     /gene="sigA"
                     /locus_tag="OO130_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SigA"
                     /protein_id="HCR5367008.1"
                     /translation="MNKIYSLKYSHITGGLVAVSELTRKVSVGTSRKKVILGIILSSI
                     YGSYGETAFAAMLDINNIWTRDYLDLAQNRGEFRPGATNVQLMMKDGKIFHFPELPVP
                     DFSAVSNKGATTSIGGAYSVTATHNGTQHHAITTQSWDQTAYKASNRVSSGDFSVHRL
                     NKFVVETTGVTESADFSLSPEDAMKRYGVNYNGKEQIIGFRAGAGTTSTILNGKQYLF
                     GQNYNPDLLSASLFNLDWKNKSYIYTNRTPFKNSPIFGDSGSGSYLYDKEQQKWVFHG
                     VTSTVGFISSTNIAWTNYSLFNNILVNNLKKNFTNTMQLDGKKQELSSIIKDKDLSVS
                     GGGELTLKQDTDLGIGGLIFDKNQTYKVYGKDKSYKGAGIDIDNNTTVEWNVKGVAGD
                     NLHKIGSGTLDVKIAQGNNLKIGNGTVILSAEKAFNKIYMAGGKGTVKINAKDALSES
                     GNGEIYFTRNGGTLDLNGYDQSFQKIAATDAGTTVTNSNVKQSTLSLTNTDAYMYHGN
                     VSGNISINHIINTTQQHNNNANLIFDGSVDIKNDISVRNAQLTLQGHATEHAIFKEGN
                     NNCPIPFLCQKDYSAAIKDQESTVNKRYNTEYKSNNQIASFSQPDWESRKFNFRKLNL
                     ENATLSIGRDANVKGHIEAKNSQIVLGNKTAYIDMFSGRNITGEGFGFRQQLRSGDSA
                     GESSFNGSLSAQNSKITVGDKSTVTMTGALSLINTDLIINKGATVTAQGKMYVDKAIE
                     LAGTLTLTGTPTENNKYSPAIYMSDGYNMTEDGATLKAQNYAWVNGNIKSDKKASILF
                     GVDQYKEDNLDKTTHTPLATGLLGGFDTSYTGGIDAPAASASMYNTLWRVNGQSALQS
                     LKTRDSLLLFSNIENSGFHTVTVNTLDATNTAVIMRADLSQSVNQSDKLIVKNQLTGS
                     NNSLSVDIQKVGNNNSGLNVDLITAPKGSNKEIFKASTQAIGFSNISPVISTKEDQEH
                     TTWTLTGYKVAENTASSSAAKSYMSGNYKAFLTEVNNLNKRMGDLRDTNGEVGAWARI
                     MSGAGSASGGYSDNYTHVQIGVDKKHELDGLDLFTGLTMTYTDSHASSNAFSGKTKSV
                     GAGLYASAIFDSGAYIDLISKYVHHDNEYSATFAGLGTKDYSSHSLYVGAEAGYRYHV
                     TEDSWIEPQAELVYGAVSGKRFDWQDRGMSVTMKDKDFNPLIGRTGVDVGKSFSGKDW
                     KVTARAGLGYQFDLFANGETVLRDASGEKRIKGEKDGRILMNVGLNAEIRDNLRFGLE
                     FEKSAFGKYNVDNAINANFRYSF"
     gene            complement(4869..5873)
                     /locus_tag="OO130_003782"
                     /pseudo
     CDS             complement(4869..5873)
                     /locus_tag="OO130_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003557916.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
     gene            5870..6100
                     /locus_tag="OO130_003783"
                     /pseudo
     CDS             5870..6100
                     /locus_tag="OO130_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1520 a         1359 c         1367 g         1854 t
ORIGIN      
        1 ggctaaaata ccgggacaga gatcaggcgt gattaatctg cagcattttt aataacagaa
       61 gtactgagct tactttacct ttgatgatgt aatagttatt tccgtcggta ctcatttttc
      121 cctgaaataa tgtcacggat gtttcttcct gctgatcctg caggcgcagt cgtaaggtca
      181 tatttttaat gagctgagat ccgggtggca aggggtattt gctgtcagat actgggcgac
      241 tgagtctgat gtggcgcgta tacagcccgt tttcgtacag ggtggcctct gttatggtgt
      301 cagtgaccag catgtgggag acaatgctgt attctttttt aaagcaccca ccagaaacgg
      361 tgattgtaat gataatggtc ttctcgatcg agatgtaaat acccgtttcg ttatactgtc
      421 cgtatcggaa ccatgagaac tcagttgcat tccagccatg ctccagaata tgtgcatttt
      481 tttgcctgat agtttctttt tttattcgct catgtttaag gcataacagg cagataaata
      541 atgtcagaaa aagaaagcta ataatgagag caagagcgat atagtctgtc acagggtaat
      601 acatattctc tccggtagaa gaagggccgc aaacgcggcc cgggctgtta tcagaaagag
      661 taacggaagt tggcgttgat cgcgttatcc acgttgtatt taccaaatgc cgatttctca
      721 aactcaagac caaagcgaag attatcgcga atttcggcgt tgagaccaac attcatgaga
      781 atacgaccgt ctttttcacc tttgatacgt ttctcaccgg acgcatcacg cagtacggtt
      841 tcaccgttgg caaacaggtc aaactggtag ccaaggccgg cgcgggctgt gactttccag
      901 tccttaccgg agaaggattt acccacatca acaccggtac gcccaatcag cggattaaag
      961 tccttatcct tcatggtcac gctcattccg cgatcctgcc agtcgaaccg tttacctgat
     1021 acggccccat aaaccagttc tgcctgcggc tcaatccagg agtcttctgt tacatgatag
     1081 cggtagcctg cttcagcacc cacatacaag gaatgagaac tgtagtcttt tgttccgagc
     1141 ccagcaaagg tcgccgagta ctcattatca tggtgaacat acttactaat caggtcgata
     1201 taggcaccag agtcaaatat agcggaagca tacagacctg ccccgacgga cttcgtcttg
     1261 ccactgaatg cattactgct ggcatgactg tcggtatacg tcatagtcag accagtgaaa
     1321 aggtcaagtc catccagctc atgtttttta tccacaccaa tctgcacatg ggtgtagttg
     1381 tcactgtatc caccagaagc tgaacctgct ccgctcatga tgcgggccca tgcaccgacc
     1441 tcgccattgg tgtcacgcag atcccccatt cgtttattca ggttgttgac ttctgtcagg
     1501 aaggctttgt aattaccgga catatacgat tttgctgcac tggaagatgc tgtattttca
     1561 gccaccttat atccggtcag ggtccacgtg gtatgttcct gatcctcttt cgtgctgatc
     1621 acaggagata tgttgctgaa acctatggcc tgagtactgg ctttaaatat ctctttattg
     1681 cttccttttg gggctgttat caggtcaacg tttaatcctg agttattatt tcccactttc
     1741 tgtatatcga ccgacagact gttattgctt ccggttaact gatttttaac aatgagttta
     1801 tccgattgat ttacagactg gctcagatca gcccgcataa tcacagcagt attagtggca
     1861 tccagtgtgt ttactgtcac agtatggaaa cccgaattct ctatgttact aaacaacaaa
     1921 agactgtcgc gggtttttaa tgattgcagg gctgactgtc cgtttactct ccataaggtg
     1981 ttatacatgc tggctgaggc agcaggagca tcaatacctc cggtataaga agtatcaaag
     2041 ccacccagca aacctgtagc cagcggtgtg tgtgtggttt tgtccaggtt atcttcttta
     2101 tactggtcaa caccaaacag aatagatgct tttttgtctg attttatatt accattgacc
     2161 caggcataat tttgagcctt taacgtggca ccatcttctg tcatattata tccatctgac
     2221 atatagattg ccgggctgta tttattattt tctgtagggg tgcctgttaa tgtcagggtt
     2281 ccggccagtt caatagcttt atctacatac atttttccct gggcggtaac agtagctcct
     2341 ttgttgataa tcaggtctgt attaattaag gataatgcac cagtcatagt aacagttgat
     2401 ttatcaccaa cagttatttt gctgttttga gcactcagac tgccgttgaa actactttcg
     2461 cctgctgaat ccccggagcg aagctgttgt ctgaatccaa aaccttcgcc agtaatgttt
     2521 cttcctgaga acatgtcaat gtatgcagtt ttatttccca gaacaatttg agagttttta
     2581 gcctctatgt gtccttttac attagcatcc cggcctatac tcagggttgc gttttctaaa
     2641 tttaatttcc ggaaattaaa tttacgactt tcccagtcgg gctgggaaaa agaggctatc
     2701 tgattgttgg acttatattc cgtattgtaa cgtttattta cagtgctttc ctggtccttt
     2761 atggcagcag aatagtcttt ttgacataaa aaaggaattg gacagttgtt attgccttct
     2821 ttaaatatgg catgttctgt cgcatgtcct tgtaatgtta actgtgcatt ccggacagag
     2881 atatcgtttt tgatatcgac tgagccatca aagatcagat tggcattatt gttatgttgc
     2941 tgggtagtat tgataatatg atttatgctt atattacctg atacattccc atggtacata
     3001 tatgcatcag tattagtaag tgataatgtt gattgcttca cgtttgagtt cgttacggtt
     3061 gttcccgcat ctgttgctgc gattttctga aatgactggt catagccgtt tagatccagt
     3121 gttccgccat ttctggtaaa atagatttcg ccattaccgc tttcgcttaa agcgtctttg
     3181 gcatttattt ttaccgtacc tttacctccg gccatgtaaa ttttattgaa ggctttttca
     3241 gcactaagga tgacagtccc attacctatt ttaaggttat ttccctgtgc tatttttaca
     3301 tccagagtac cactacctat tttatgcaga ttatctccgg caacgccctt aacattccat
     3361 tcaacggtgg tattattatc aatatctatc ccggcacctt tataagactt atcttttccg
     3421 tacactttat atgtctggtt cttatcgaat ataagcccgc caatgccaag atcggtatcc
     3481 tgcttgagcg ttaattcccc tcctcctgag acagacaggt ccttatcttt tataatcgat
     3541 gataactctt gttttttacc atccagctgc atagtgtttg tgaaattctt ttttaaattg
     3601 tttaccagaa tattattaaa taacgagtag tttgtccagg ctatattggt actactgata
     3661 aaaccaactg tactggtaac accatggaaa acccattttt gttgttcttt atcatataga
     3721 taagaaccag aaccactatc gccaaaaatt ggtgagtttt taaaaggggt tctgttggta
     3781 taaatgtaac tcttgttttt ccagtccaga ttaaaaagac ttgcgcttaa caagtcggga
     3841 ttatagtttt gtccaaacag atattgtttg ccgtttaata tcgttgaggt tgttccggca
     3901 cctgctctga agccaattat ttgttcctta ccgttgtagt ttacgccata tcttttcatc
     3961 gcatcttcgg gagaaagtga gaagtcggca ctctccgtaa cccctgttgt ttccacgacg
     4021 aatttattca gacgatgaac cgaaaagtcg ccagatgata ctctgttact tgctttatat
     4081 gctgtctgat cccatgactg tgttgttatt gcatgatgct gtgtaccgtt atgagtcgca
     4141 gtaacactgt acgcacctcc aattgatgtt gttgcacctt tgttggaaac agcagaaaaa
     4201 tcaggtacag gtagttctgg aaaatgaaat atctttccat ctttcatcat taattgaaca
     4261 tttgttgcac ccggtctgaa ctctcctctg ttttgagcaa ggtcaagata atcgcgggtc
     4321 catatattat ttatatccag cattgctgca aatgctgttt caccataact tccatatatt
     4381 gaggataaaa taataccgag gataactttc tttcttgatg taccgacact aactttccgg
     4441 gtcagttcag aaacagcaac taatccacct gtaatatgac tatatttcag tgaataaatt
     4501 ttattcatac gtgaactccg atatttctga gttgtggtta tgggatagca tttaattaca
     4561 acggggaact atcagtcatg gtacgtaact gataagggta taatgataat cactgtcagt
     4621 tatatttgta tgacagatgt aacaaaatga aaaaaaagat aagcacaagt gattgattgg
     4681 tcagatattg atcacctggt gtttgtattt tgacctttgc gatcaaaatt tgatctttgt
     4741 tgtttccggt caatccttta caggttttct gtattatccg ggggagttaa gtctgtcgat
     4801 gtccgacaga cttttgatga tggaaacaac atgcagcgat aatgtgcatg ctgttttgca
     4861 ggtggaactc agaaggtgaa ctggaactgt gcgaccgcac cgtaagtgtt gtccgtgccg
     4921 atcaggcctg caacatcaag gtgaaccaca tcaaacggtg agatccccac cccggccgtg
     4981 aatgcactgc cgtcgttacc ggccagattc tgacggtaac cggcgcgcag ctgtgcccat
     5041 ttccaggcat taaattctgc gccaatggcg gcaaactgac ggttgctgtc ggaggtgaaa
     5101 ccgctggccg gggtcagatc cacatcaaat gcggtggtga acatcgcgtt gtgccaggag
     5161 acaccggccg tcacctgcgg gcggatcctg aaggtttccg tgataccgtt cacttctttt
     5221 gtctcaatac tacgggggat caggttctgt gcgaccagtc ccagcgtcca gttgtcatcc
     5281 aggttcgtac tggcaccgat gtcggcgttg atgccatttt tcgtgttgtg gtagcggtcg
     5341 cctttgaagg cgctgctgtc ataatttctg accagcacgt tgtagttgaa caggtcgacg
     5401 cgctggaatt tgggggggaa gcccagagac catttctgcc cggccgtttc cagttccgta
     5461 gccagagcaa tacccacatc agtgatcagt gccgcgcggg caaaggcctg tgatgtcagg
     5521 cggcttttgt ccacctcctg cccggtgctg tttgccacct cttccaggta agtgaggtca
     5581 gcatcagaga ctttaccgtc gacactgacg gtgccgtgtg atttaaccat cagtgcggca
     5641 gagaggcgat caccgggaag ggccgccaca gcagaaacgc ccagctgtgc ggcggcatgt
     5701 gtgttacgga agtcccggag gtgttctttc aggcgtgcgg cagcctcagg aacaccgtgg
     5761 ttactttcga tggcgcggtc gaaggccttc cactcatcac tgatccggtc ggcgttgtcg
     5821 gtgatattat ccggattgga gagctgcgcg ccgacggacg gaagcaggat gctgggctca
     5881 tgcgcgacgc aaaatccacg atgtatatat cagtactaaa agcgcgacgg cggaagaagc
     5941 tctgaaacta atcggtgaac tgtacgccat agagcacgat atacgcgggt taccggtgtc
     6001 tgaacgcctg gcggtcaggc aaatgcagag tagaccgctc ctgacttccc tgtataagct
     6061 gatgcaggag aaagaacaca cgttatcgaa gaaatgtcgt
//
LOCUS       DAJTMC010000205         6053 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.205, whole genome shotgun sequence.
ACCESSION   DAJTMC010000205 DAJTMC010000000
VERSION     DAJTMC010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6053
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.205"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(97..2199)
                     /locus_tag="OO130_003784"
     CDS             complement(97..2199)
                     /locus_tag="OO130_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HCR5367009.1"
                     /translation="MKIFSVRQTVLPALLVLSPVVFAADEQTMIVSAAPQVVSELDTP
                     AAVSVVDGEEMRLATPRINLSESLTGVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVTTQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPSDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDEASKLSLIFNSVDIKADDPGGLTKAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSAQDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNSGMPEYGQKGELRRDERN
                     LMWNIDPYLQTQWQLSEKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNIYLAVGRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVNA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PSPGRNYGVGMNIAWRFE"
     gene            2441..3502
                     /locus_tag="OO130_003785"
     CDS             2441..3502
                     /locus_tag="OO130_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HCR5367010.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGATINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKESVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            complement(3615..5114)
                     /gene="ansP"
                     /locus_tag="OO130_003786"
     CDS             complement(3615..5114)
                     /gene="ansP"
                     /locus_tag="OO130_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HCR5367011.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKEGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETAS"
     gene            5381..5998
                     /locus_tag="OO130_003787"
     CDS             5381..5998
                     /locus_tag="OO130_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HCR5367012.1"
                     /translation="MIKVYGVPGWGSTISELMLTLADIPYQFVDVSGFDHEGTSRELL
                     KTLNPLCQIPTLTLENDEIMTETAAIALMVLDRRPDLAPPVGRAERQQFQRLLVWLVA
                     NVYPTFTFADYPERWAPDAPEQLKKNVIEYRKSLYIWLNSQLTAEPYAFGEQLTLVDC
                     YLCTMRTWGPGHEWFQDNATNISAIADAVCQLPKLQEVLKRNEII"
BASE COUNT         1612 a         1535 c         1442 g         1464 t
ORIGIN      
        1 ttgtcttgct gaaattatgc aacaaaatat tgctatttta taccagcaat acaatcgtta
       61 aagtaaatgc ccggcaaata ccgggcatca aggtaattac tcaaatctcc acgcaatatt
      121 catgccgaca ccataatttc gtccgggcga aggttcgtaa tatcgcccgt ttgactcatt
      181 gacaatgaca gaaccaacgt attctttatc gaataaatta tcgacacgac caaataaatc
      241 cacagttaaa ttgtggtaat tgtatttata cccggtgaat aagccgacga gagtataaga
      301 cggcgctttt gccgtatttt catcatctgc cataatatcg cccatataac gcgcttctgt
      361 gcctgcatac caaccatctt ccggtacata acctatcgac gcaaagccca tattacgggc
      421 gatccctggc atccgattac cgttacaatc ctgttcattg caaacattgc tgcgataggt
      481 cgcatcaagc caggtccagg acgcgtttac gcgaaaatct cctgcgaaac gttgatccca
      541 tgccagttca gcgccttgac gacgggtctt tccggcattt ttgtaagtcg tacgcccacc
      601 gctactgcta tcgacaacaa tttcatcatc agtgtcggtc tgaaacaatg cgagactaag
      661 cagcccatca ccaatacgcg ttttactgcc gatctcaatt gtatcgttgg tggatggttt
      721 taaaccaaag ttcataccgc tttgcccatc agcacgataa gacagctcat taatcgtcgg
      781 cgtttcaaaa cctcgcccga ctgccagata gatattccag gcatcggtca ttgcatattt
      841 taacgaaccg gcaggtagcc atttatgata actggcatca ccgctgtcat cgccgttacc
      901 cggagtaacg taatggtcgt tggaatcaaa ccacacggag ctgtagcgca cgccagcatc
      961 cagcgacagt ttttcgctca gctgccactg cgtctgtaaa tagggatcga tgttccacat
     1021 cagattgcgt tcgtcgcgac gcaactcacc tttttgcccg tactccggca tgccgctatt
     1081 caggcggaag ttattgtagc ccttgcggtt ttcactcatg ttttcgtagt tcaggccggt
     1141 agtgaacgtg accggaacgc ccagttcacc acggtgtgtc cagcggctgt ctatcccctg
     1201 gtaatggcgt tgcagggtaa tcacgccgcc cgcatgtgac gggttaagtt gtggtgccat
     1261 gggtattgac tggtactggg tcgtttctcg ctctccggca tacatcatca cactcatatc
     1321 atcttgcgcg ctcaggctac gctcatagcg caaccctgcc tgagtttgct tgatggtttt
     1381 tcgcgtgtcg tactgttctg cacgaggcgc ttgttgtgga ttagccttcc attctgcttt
     1441 ggttagccca cctgggtcat ctgctttgat atccacacta ttgaaaatca gacttaattt
     1501 gctggcttca tcaatgcgta cgcccagttt ggcattggct aaatttttct gtgcgccact
     1561 atggtcacga tagccgtggg tcgtaaaacg cgtggttgag acggtgtaat cgacatcgct
     1621 aggctgtgtg ccgtctcccg ttgcgcccgt tgctttcagc ccatagcgcc agctgccaaa
     1681 actgccgtag taactactgg cttcaatggt tggtggctgt tgtccggtct gggtggtgac
     1741 attcattacc ccaccagacg cgttgccata cagggcagag aaggggccac gcagcacttc
     1801 cacattttgc acactgctta aatcgatgtt ggatgtttgc ccttgcccgt cgggcatggt
     1861 ggcgggaata ccgtccacat acaggcgaat accgcgaata ccgtaagtgg agcgggagcc
     1921 aaatccgcga atcgacagct gtaaatcttg cgcatagttc tgccggtttt gtacctgcaa
     1981 accaggcacg ccggtcagtg attcggacaa gttaatgcgc ggtgttgcca ggcgcatctc
     2041 ctcgccatcc accacgctta ctgctgctgg ggtatccagt tctgaaacca cctgcggtgc
     2101 ggcactgaca atcatagtct gttcatcagc ggcaaaaaca acgggggaaa ggacaagcag
     2161 tgcgggcaaa acggtctgtc ggacggaaaa aatcttcatg aaaaaagcca ggttaagaat
     2221 gggaaaacgc tgtcatggta ataaaattgt aaacttatgc aaaacgaaac ggcacgatac
     2281 gttaattaat tgagaaaatt gtagtcgtaa cggcaagaaa tgctccacat ttgagaaaat
     2341 aatgattacc attcccattt ataacaagag cgtgacgatg attacgctta gcgaagcatt
     2401 gtgaagcagc aaaaatattg gttcatcaaa gggagtcgtc atgcatttac gtcatctgtt
     2461 ttcatcgcgc ctgcgtggtt cattactgtt aggttcattg cttgttgctt catcattcag
     2521 tacgcaggcc gcagaagaaa tgctgcgtaa agcggtaggt aaaggtgcct acgaaatggc
     2581 ttatagccag caagaaaacg cgctgtggct cgccacttcg caaagccgca aactggataa
     2641 aggtggcgtg gtttatcgtc ttgatccggt cactctggaa gtgacgcagg cgatccataa
     2701 cgatctcaag ccgtttggtg ccaccatcaa taacacgact cagacgttgt ggtttggtaa
     2761 caccgtaaac agcgcggtca cggcgataga tgccaaaacg ggcgaagtga aaggccgtct
     2821 ggtgctggat gatcgtaagc gcacggaaga ggtgcgcccg ctgcaaccgc gtgagctggt
     2881 agctgatgat gccacgaaca ccgtttacat cagtggtatt ggtaaagaga gcgtgatttg
     2941 ggtcgttgat ggcgagaata tcaaactgaa aaccgccatc cagaacaccg gtaaaatgag
     3001 taccggtctg gcgcttgata gcaaaggcaa acgtctttac accactaacg ctgacggcga
     3061 attgattacc atcgacaccg ccgacaataa aatcctcagc cgtaaaaagc tgctggatga
     3121 cggcaaagag cacttcttta tcaacatcag ccttgatacc gccaggcagc gtgcatttat
     3181 caccgattct aaagcggcag aagtgttagt ggtcgatacc cgtaatggca atattctggc
     3241 gaaggttgcg gcaccggaat cactggctgt gctgtttaac cccgcgcgta atgaagccta
     3301 cgtaacgcat cgtcaggcag gtaaagtcag tgtgattgac gcgaaaagct ataaagtggt
     3361 gaaaacgttc gatacgccga ctcatccaaa cagcctggcg ctgtctgccg atggcaaaac
     3421 gctgtatgtc agtgtgaaac aaaaatccac taaacagcag gaagctaccc agccagacga
     3481 tgtgattcgt attgcgctgt aatagatatg gcgagggtga aaacggtgag acttgcctgc
     3541 cttttatcac ggcgtatccg gcactttcgt cagtaatctg aatttgcccg gcacgtagcc
     3601 gggcaatttt gcaattaact cgccgtttca ggcttaaaca caatttcctg tttttcttca
     3661 tcttcctcga cgactggcgc agtgctgtga atttcagcaa cgcgtttgcg cacaccaaac
     3721 cagcctataa ccagcagaat accgataatc ggcagcgccg cgatagtata agtcccgttc
     3781 gggtaatcga acgccatcag cacaaggaca ctcagtaaaa acagtaatgt cagccaggaa
     3841 gtgaagggcg cgccaggcag tttaaaactg acatccgctg ctttgccttc tttaatcgct
     3901 ttacgcaggc gcatctggca cacgatgata aacgcccatg aagcgatgat tcccagcgac
     3961 gcgaagttca acacaatctc aaatacgcgc gacggcacca gatagttgag gaatacgccg
     4021 acgacataca caaccagtgt cgctagaatc ccggcatacg gcacatgctg acgactcatt
     4081 ttcgccataa aactcggtgc ggaaccgccc atcgccattg agcgcagaat acgtccggtg
     4141 cagtacagac ctgagttcag gctggagagg gcagcggtca gcaccacaat gttcataatg
     4201 ctgccgatat atggcacacc cagtttagag aaaaacgtca cgaacggact ttgccccgcc
     4261 tgatacgcgc tccacggcaa taacataacc agcaacacca cggagccgac gtaaaacagg
     4321 ccaatacgcc aaatcacact gttaatggct ttaggcacca tggtctgcgg atctttacat
     4381 tctccggcag ctgtacccac catttcaatg gaggcaaaag caaacactac gccctgaatc
     4441 aacaccagcg caggcagcaa accgtggggg aagaagccgc cattatcggt gattaaatga
     4501 aagccagtgg tgttgccatc cagcggctga ccactaccga ggaacactgt acccacgacc
     4561 aaaaaggtca caatggcgag cactttaata agcgcaaacc agaactccat ctccgcaaac
     4621 catttcacac cgatcatatt catggtgcca acgatggtaa gtgcagcgag cgcaaagacc
     4681 cactgcggca cgccgccaaa cgcaccccag taatgcatat acagagcgac ggcggtaata
     4741 tcaacaatcc ccgtcatcgc ccagttgatg aagtacatcc agccagcaac ataagcggct
     4801 ttctcaccca aaaactcacg ggcataagag acaaaactgc cactggaagg gcggtgtagc
     4861 accagctcac ccaatgcacg cagaataaaa aacgaaaaca agccacaaat taaataaacc
     4921 agtgccagtg ctggacccgc catttgcagt cgggctcctg cacctaaaaa caagccggtg
     4981 ccaatcgcgc cgccaatggc gatcatctgt acctggcgat tgcccatcgc tttgtgatac
     5041 ccctcttcgt gggcattaag ccagcggcgt ttcgcggcgt gttgatctga agtgtcggtg
     5101 tcgtgtttac tcattgctct ccctgattgc tttaatgaaa aagtcatata agttgccatg
     5161 aacaatgttt attcatcctg gcgatgatag ttatcaccat tattctttat gggtataaaa
     5221 ctatcggcgc agcatcctac ccgttatcga tatacgatgc aaaacatccc cttacaatcc
     5281 tgaaggggat tcatacaact gacgaaaaaa tgacaaatac ttttgctggt taacctgtgt
     5341 actgtcctac acttaatctt taaaagattg tgaggggcat atgattaagg tgtatggcgt
     5401 acccggatgg ggctctacaa tcagtgagct gatgctaaca ctggctgata ttccttatca
     5461 gttcgttgat gtcagtggtt ttgaccacga gggaacttca cgcgagttat tgaaaaccct
     5521 gaacccgttg tgtcagattc cgacactgac gctggaaaat gacgagatta tgacggagac
     5581 agcggcgatt gcattgatgg ttctcgatcg tcgcccggac cttgcgccac cggttgggcg
     5641 cgccgaacgt cagcagtttc aacggctatt ggtctggctg gttgctaatg tctatccaac
     5701 attcactttc gccgattacc ctgagcgttg ggctcctgac gcacctgaac agttaaagaa
     5761 gaatgttatt gaatatcgta aatcgcttta tatctggctg aattcacaat tgaccgctga
     5821 accttatgcg tttggtgagc aactaacgct ggtggactgt tatctttgca ctatgcgcac
     5881 atgggggcct gggcatgaat ggtttcagga taatgccacg aatatcagtg cgattgctga
     5941 tgctgtatgt cagttaccga aattacaaga agtattaaaa aggaatgaaa ttatttgagt
     6001 gggataccag aggattatgt tatttattat tcagtaactt tatccacaat gat
//
LOCUS       DAJTMC010000206         6049 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.206, whole genome shotgun sequence.
ACCESSION   DAJTMC010000206 DAJTMC010000000
VERSION     DAJTMC010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6049
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.206"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(24..392)
                     /locus_tag="OO130_003788"
     CDS             complement(24..392)
                     /locus_tag="OO130_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5367013.1"
                     /translation="MNMMRIFYIGLSGVGMMFSSMASGHDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSLQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQITTKMLFMCRE"
     gene            918..1613
                     /gene="minC"
                     /locus_tag="OO130_003789"
     CDS             918..1613
                     /gene="minC"
                     /locus_tag="OO130_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HCR5367014.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            1637..2449
                     /gene="minD"
                     /locus_tag="OO130_003790"
     CDS             1637..2449
                     /gene="minD"
                     /locus_tag="OO130_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HCR5367015.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            2453..2719
                     /gene="minE"
                     /locus_tag="OO130_003791"
     CDS             2453..2719
                     /gene="minE"
                     /locus_tag="OO130_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HCR5367016.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(3198..3317)
                     /locus_tag="OO130_003792"
     CDS             complement(3198..3317)
                     /locus_tag="OO130_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HCR5367017.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(3278..3463)
                     /locus_tag="OO130_003793"
     CDS             complement(3278..3463)
                     /locus_tag="OO130_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5367018.1"
                     /translation="MSRRYSSATITLVILFCRRPHCRKISVRQYYLKRVIFYPTTLQR
                     TAFKKCLFLMRDIIISS"
     gene            3564..3737
                     /locus_tag="OO130_003794"
     CDS             3564..3737
                     /locus_tag="OO130_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5367019.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVRATYLIPDI"
     gene            3739..4083
                     /locus_tag="OO130_003795"
     CDS             3739..4083
                     /locus_tag="OO130_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HCR5367020.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            4087..4422
                     /locus_tag="OO130_003796"
     CDS             4087..4422
                     /locus_tag="OO130_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HCR5367021.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(4479..>6049)
                     /locus_tag="OO130_003797"
     CDS             complement(4479..>6049)
                     /locus_tag="OO130_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR5367022.1"
                     /translation="LVNNTGGDLNIISSDSILNGATKLVSGTTTLKLSENTIWNMKDD
                     SVVTHLTNSDSIINLSYDDGQTFTQGKTLTVKGNYVGNNGQLNIRTVLGDDKSATDRL
                     IVEGNTSGSTTVYVKNAGGSGAATLNGIELITVNGDESPAEAFRQGDARIAAGAFEYQ
                     LKQQGKNWYLTSYQSVNEEDNSSEGNSEPTETPTPVLRPEAGSYVANLAAANTLFVMR
                     LNDRAGEMRYIDPVTEQERSSRLWLRQIGGHNAWRDSNGQLRTTSHRYVSQLGADLLT
                     GGFTDSDSWRLGVMAGYARDYNSTHSSVSDYRSKGSVRGYCAGLYATWFADDISKKGA
                     YIDAWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYGFALNKSFGLEAAKYTWIFQ
                     PQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRTFIRTQEKNSGPHGDDFEPF
                     VEMNWIHNSKDFAVSMNGVKVEQDGASNLGEIKLGVNGNLNPAASVWGNVGVQLGDNG
                     YNDTAVMVGLKYKF"
BASE COUNT         1662 a         1396 c         1384 g         1607 t
ORIGIN      
        1 tagaacataa tgtaaacaag gcatcactca cgacacataa ataacatctt tgtggtgatt
       61 tgattgattt ttccactccg cttaccatcc acaccaaaat aacgatcatc acgacataat
      121 ttttctttaa catcgcaaaa tacaccgtta gcgaatgtaa attcgctggg atcgtagcct
      181 tgtaatgatt gtaagttttc agcggctttt tcgccgagat atttccttgt taactctggg
      241 gaaataccat ctgagttcac acaaatatac gggtcgcaaa caacgccgca cgccggagat
      301 tgaagtccgc cagcgtcatg tccagacgcc attgatgaga acatcatccc cacacctgac
      361 aatccgatat aaaaaatacg catcatattc atagcacatc ctgataatcc aaccgtacaa
      421 cacttatgct catttcatca tcaatccgta atcaaaaccg ctgacctgga cgagttacga
      481 tgacctgaca gaagtatcag acgtcttctg cggtttctat gattatgata gagcaattct
      541 atggcggata ttaagataac gggtatctgt tctttaatga tgctgcaaaa aatggaaagt
      601 taagaaagcc tggcgtggtg aaaaaataca cttgcctcaa tactggatat ttaaacaggt
      661 ctggagatgc tgtcacgatt agggtgcgga aaaaggaagc gtaaataaga acaatcaaaa
      721 gtacgacggc aatgggttga ttgacaaggg tattttttaa gctatgaatc agcgccattt
      781 atcacagaat agacttttac tctgaataaa tgggagggtg acttgcctca atataatcca
      841 gactataaca tgccttatag tcttcggaac atcatcgcgc gctggcgatg attaatagct
      901 aattgagtaa ggccaggatg tcaaacacgc caatcgagct taaaggcagt agcttcactt
      961 tatctgtggt tcatctgcat gaggcagaac ctaaggttat ccatcaggcg ctggaagaca
     1021 aaatcgctca ggcccccgca tttttaaaac atgcccccgt tgtactcaac gtcagtgcac
     1081 tggaagaccc ggtaaactgg tcagcgatgc ataaggcggt gtcggcaacc ggtttgcggg
     1141 ttattggcgt aagtggctgc aaagatgcgc aacttaaagc cgaaattgaa aagatggggc
     1201 tgcctatcct gacggaagga aaggaaaaag cgccgcgtcc agctcccgca ccacaggctc
     1261 cagcgcaaaa tacaacgccg gtcacaaaaa cgcgtttaat agataccccg gtgcgttccg
     1321 gtcagcgtat ttatgctcca caatgtgatc tgattgttac aagccacgtt agcgctgggg
     1381 ccgaattgat tgccgatggg aacattcatg tctatggcat gatgcgcggt cgtgcgcttg
     1441 caggggcaag tggtgaccgg gaaacgcaaa tattttgtac gaacctgatg gcggaactgg
     1501 tgtccatcgc aggtgaatac tggctgagtg atcaaatccc agcagaattt tatggcaaag
     1561 cggcgcgact gcagttagtc gaaaacgctt tgaccgttca accgttaaat tgatcccttt
     1621 ttaacaagga atttctatgg cacgcattat tgttgttact tcgggcaaag ggggtgttgg
     1681 taagacaacc tccagcgcgg ccatcgccac tggtttggcc cagaagggaa agaaaactgt
     1741 cgtgatagat tttgatatcg gcctgcgtaa tctcgacctg attatgggtt gtgaacgccg
     1801 ggtcgtttac gatttcgtca acgtcattca gggcgatgca acgctaaatc aggcgttaat
     1861 taaagataag cgtactgaaa atctctatat tcttccggca tcgcaaacac gcgataaaga
     1921 tgccctcacc cgtgaagggg tcgccaaagt tcttgatgat ctgaaagcga tggattttga
     1981 atttatcgtt tgtgactccc cggcagggat tgaaaccggt gcgttaatgg cactctattt
     2041 tgcagacgaa gccattatta ccaccaaccc ggaagtctcc tcagtacgcg attctgaccg
     2101 tattttaggc attctggcgt cgaaatcacg ccgcgcagaa aatggcgaag agcctattaa
     2161 agagcacctg ctgttaacgc gctataaccc aggccgcgta agcagaggtg acatgctgag
     2221 catggaagat gtgctggaga tcctgcgcat caaactcgtc ggcgtgatcc cagaggatca
     2281 atcagtgttg cgcgcctcta accagggtga accggtcatt ctcgacatta acgccgatgc
     2341 gggtaaagcc tacgcagata ccgtagaacg tctgttggga gaagaacgtc ctttccgctt
     2401 cattgaagaa gagaagaaag gcttcctcaa acgcttgttc ggaggataag ttatggcatt
     2461 actcgatttc tttctctcgc ggaagaaaaa cacagccaac attgcaaaag aacggctgca
     2521 gattattgtt gctgaacgcc gtcgcagcga tgcagaaccg cattatctgc cgcagttgcg
     2581 taaagatatt cttgaggtca tttgtaaata cgtacaaatt gatcctgaga tggtaaccgt
     2641 acagcttgag caaaaagatg gcgatatttc tattcttgag ctgaacgtga ccttaccgga
     2701 agcagaagag ctgaaataag cccgctgtaa aaacgcattt atcttcaagg cagagtcatt
     2761 tctgccttga gtttttcatc cctctcatcc acgttgtggt aaagcggcga gtattcttgc
     2821 tgatactcct cattgctatt tccacctccc cctttacacc ttaaggctgt tcccacgcac
     2881 agaacaaaat gtttatgcct ggttgagtaa ataacctcat tgttagtgtg gatacactgg
     2941 gggatgataa ccccgcaatt tcattattca ttgttctccc ataggatgag gtttcccgcc
     3001 gtaactggcg ggcttttttt gccccagaat tttccctttt agccccctgt aaacgaaaac
     3061 tgcgccgaag cgcagtttaa tcgttgatgg tcagaacgac cagttcacga tatatactgc
     3121 gggtatcgac tgatttcgat tccatgccat tggataaatg atagtagcgt ctaagtgacc
     3181 taagctctgc tgcaacatca gcccgcaggc accagaccag gggattcatc ctgaagagac
     3241 agcgcaggtg tattgtgttc accactcacc aaagacatca tgatgaaatg atgatatccc
     3301 gcataagaaa gaggcatttt ttaaacgcag tgcgctgaag tgtagttgga taaaaaatca
     3361 ctcttttcag ataatactgt cgtacgctta tctttcgaca atgaggccgt cggcaaaata
     3421 aaatgactaa tgtaatcgtt gctgatgaat atcttctgct cacataaaaa tcacacaata
     3481 atattgataa atcagattat cttaccttta caacattgcg ttaacagtca atgacacttc
     3541 ttgaataaaa atattgccaa tcaatgagtt attcctcatt taagattatt ctcattcacg
     3601 taaaaaacat aattcctatc atcacagcca cgctcatttt gaattatctg aataatagcg
     3661 agcgttcttt agttaagcag atcttaattg aagacgaaat tattgtgcgt gcaacttact
     3721 taataccgga catctaagtt gaagaaaaaa atacttgcct tcgggcttat ctcagcattg
     3781 ttctgctcca cgccagcgat ggcggatatg aatcgcacca cgaaaggtgc attgctaggc
     3841 gcaggcgttg gtttacttac tggcaatggt gttaatggcg tactgaaagg tgcagctgtt
     3901 ggcgctggtg ttggtgcagt aacagaaaaa ggccgcgacg gtaaaaatgc gcgtaaaggt
     3961 gcaaaagtag gtgccgccgt tggtgctgtc actggcgttc tgaccggcaa tggcctcgaa
     4021 ggggcgatta aaggtgccgt gattggcggt acgggtggtg caattctggg taaaatgaaa
     4081 taaggaatga tgatgaaaaa atttgcttta ctggcgggcc tgtttgtttt cgcaccaatg
     4141 acctgggcgc aggactacaa tattaaaaac ggtttaccgt cagaaaccta tattacttgc
     4201 gcagaagcca acgaaatggc aaaaactgac agcgcacagg ttgcagaaat tgttgcggta
     4261 atgggtaatg ccagcgttgc cagccgtgat ttaaaaattg agcaatctcc ggaactgagc
     4321 gccaaagtgg tagaaaaact gaatcaggtt tgcgctaaag acccgcaaat gctgttgatc
     4381 acggcaattg atgacaccat gcgtgctatc ggcaaaaaat aatatttcat atgtaccgcg
     4441 tcgatgaata cgacgcggtt ttttagctaa cggcgggatc agaacttata tttcaggccc
     4501 accatcactg cggtgtcatt gtagccatta tcacccagct gcacgcccac attcccccag
     4561 acgctggccg ctggattcag gttgccattt acgccaagtt taatttcccc caaattactc
     4621 gccccatctt gttcgacttt cacaccgttc attgagacag caaaatcttt actgttatgg
     4681 atccagttca tttcaacaaa aggttcaaag tcgtcaccgt gcggaccgct gtttttctcc
     4741 tgagtacgaa taaaggtgcg gaaaccaagt cgggtctgga tgttgttatt tgcgtcattc
     4801 tcaatacgtg agccattggc ttcggtgtgc gcattatgat cgacgcccat ccagatagcc
     4861 tgtgcctgtg gctggaatat ccacgtgtat ttcgccgctt ccagaccaaa ggatttattc
     4921 agggcaaagc cgtaacccgc ttccagcgag acggttgcgc ctttcgcact ataggattca
     4981 taggctaatt catccccttt caccgagttt ttaaaccagc tatattgcgc ccaggcgtca
     5041 atgtatgcgc cttttttact gatgtcatcg gcaaaccaag tggcatacag ccctgcgcaa
     5101 tagcctctga cactcccttt cgaacgataa tccgacacgc tggaatgagt tgagttgtag
     5161 tcgcgggcat aaccagccat cactcccaaa cgccaactgt cactgtcggt aaaaccaccg
     5221 gttaacagat cggcccctaa ctgcgagacg tagcgatgcg aggtcgttct caactgtccg
     5281 ttgctgtcac gccaggcatt atgcccgcca atttgacgta gccaaagtcg gcttgaacgc
     5341 tcctgttcag ttacaggatc gatgtagcgc atttcacccg cacggtcgtt cagacgcata
     5401 acaaaaagag tgttagcggc tgccaggttg gcgacgtaac ttccagcttc ggggcgcagg
     5461 acgggggtag gcgtttctgt tggttctgaa tttccctctg agctgttatc ctcctcattc
     5521 actgactgat aacttgtcaa ataccagttt ttgccttgtt gttttagttg atattcgaaa
     5581 gctccagctg caatccttgc atcaccttgt ctgaaggctt ctgctggaga ttcatcgcca
     5641 ttcacagtta tgagttcgat cccgtttagc gtggccgcgc cgcttcctcc agcatttttc
     5701 acatagacgg tagttgaacc cgaagtatta ccctcaacaa taagtctgtc cgtagccgat
     5761 ttatcatcac ctaatacggt gcggatatta agctgaccat tattaccgac ataattacct
     5821 tttacggtta atgtttttcc ttgggtaaat gtttgaccat catcatacga aagattgata
     5881 atactgtctg aattagtcag atgggtaaca acggagtcat ctttcatatt ccagattgta
     5941 ttttctgaaa gcttcagcgt ggttgtgccg ctaacgagtt tagtcgctcc atttagaata
     6001 gagtcggacg aaattatgtt taagtcacct ccagtattat taaccagaa
//
LOCUS       DAJTMC010000207         5911 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.207, whole genome shotgun sequence.
ACCESSION   DAJTMC010000207 DAJTMC010000000
VERSION     DAJTMC010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5911
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.207"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(<1..406)
                     /gene="dsbG"
                     /locus_tag="OO130_003798"
     CDS             complement(<1..406)
                     /gene="dsbG"
                     /locus_tag="OO130_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HCR5367023.1"
                     /translation="MLKKILLLALLPAIAFAEELPAPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQ"
     gene            778..1341
                     /gene="ahpC"
                     /locus_tag="OO130_003799"
     CDS             778..1341
                     /gene="ahpC"
                     /locus_tag="OO130_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HCR5367024.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            1512..3077
                     /gene="ahpF"
                     /locus_tag="OO130_003800"
     CDS             1512..3077
                     /gene="ahpF"
                     /locus_tag="OO130_003800"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HCR5367025.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(3198..3626)
                     /gene="uspG"
                     /locus_tag="OO130_003801"
     CDS             complement(3198..3626)
                     /gene="uspG"
                     /locus_tag="OO130_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HCR5367026.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLTQDDGVIHLLHVLPGSA
                     SLSLDRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            3847..5085
                     /locus_tag="OO130_003802"
     CDS             3847..5085
                     /locus_tag="OO130_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent formaldehyde
                     dehydrogenase"
                     /protein_id="HCR5367027.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITATAICGSDLHL
                     YRGKIPQVKHGDIFGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRMQQYA
                     ACENTNAGKGAALNKKQIPAPAALFGYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNAQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLSFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGLTFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQS
                     AEEAQKAVSGLVNAMPGGTI"
     gene            complement(5316..5726)
                     /gene="rnk"
                     /locus_tag="OO130_003803"
     CDS             complement(5316..5726)
                     /gene="rnk"
                     /locus_tag="OO130_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HCR5367028.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
BASE COUNT         1524 a         1408 c         1533 g         1446 t
ORIGIN      
        1 cctgctgcca gaactgttta caatatgggc agaacggatc ggcgaagacg tagacaatga
       61 ccggcgcatc ttttttaccg tcgaggagcc agtgggattg ttccatccgt tgccacattt
      121 cgcgtccggc tggtgcgtaa atttcttttt cgataagtgt gttactcagg ttttcacctt
      181 tctcgttgta catgtaacca gagatagcgt gcttaccatc tggagtcagg tagatggtga
      241 cgcccatatc ctgatacttt ccgagataac ctttcattcc tccgggggca tcgaacgttt
      301 tgatgattgt aatgccctgt ttttcaatcg cttttactgg agcaggaagt tcctctgcga
      361 aggcgattgc aggaagcaga gccagtaaaa gtatcttttt taacattaat ttgtcctttt
      421 cagtcagtgc aaaagtcgag taaaaggcat aacctatcac tgtcataggt aagagcttag
      481 atcaggtgat tgccctttgt ttatgagggt gttgtaatcc atgtcgttgt tgcatttgta
      541 agggcaacgc ctcagcctgc aggcaggcgc tgaagatacc aaagggtagt tcagattaca
      601 cggtcacctg gaaagggggc cattttactt tttatcgccg ctggcggtgc aaagttcaca
      661 aagttgtctt acgaaggttg taaggtaaaa cttatcgatt tgataatgga aacgcattag
      721 ccgaatcggc aaaaattggt taccttacat ctcatcgaaa acacggagga agtatagatg
      781 tccttgatta acaccaaaat taaacctttt aaaaaccagg cattcaaaaa cggcgaattc
      841 atcgaaatca ccgaaaaaga taccgaaggc cgctggagcg tcttcttctt ctacccggct
      901 gactttactt tcgtatgccc gaccgaactg ggtgacgttg ctgaccacta cgaagaactg
      961 cagaaactgg gcgtagacgt atacgcagta tctaccgata ctcacttcac ccacaaagca
     1021 tggcacagca gctctgaaac catcgctaaa atcaaatatg cgatgatcgg cgacccgact
     1081 ggcgccctga cccgtaactt cgacaacatg cgtgaagatg aaggtctggc tgaccgtgcg
     1141 accttcgttg ttgacccgca gggtatcatc caggcaatcg aagttaccgc tgaaggcatt
     1201 ggccgtgacg cgtctgacct gctgcgtaaa atcaaagcag cacagtacgt agcttctcac
     1261 ccaggtgaag tttgcccggc taaatggaaa gaaggtgaag caactctggc tccgtctctg
     1321 gacctggttg gtaaaatcta aatttccttc gtctttcgcg ctaccaactc cggcgcgaaa
     1381 tacttcggaa attcacctaa ttcttcgggt gctgcggcac ccgattttct tccccgcact
     1441 atgatgcaag ctgcatccag gtagccgtag aggccgcttg catgatgatg tttaaagccc
     1501 aggagataaa catgctcgac acaaatatga aaactcaact caaggcttac cttgagaaat
     1561 tgaccaagcc tgttgagtta attgccacgc tggatgacag cgctaaatcg gcagaaatca
     1621 aggaactgtt ggctgaaatc gcagaactgt cagacaaagt cacctttaaa gaagataaca
     1681 gcttgccggt gcgtaagccg tctttcctga tcaccaaccc aggttccaac caggggccac
     1741 gttttgcagg ctccccgctg ggccacgagt tcacctcgct ggtactggcg ttgctgtgga
     1801 ccggtggtca tccgtcgaaa gaagcgcagt ctctgctgga gcagattcgc catattgacg
     1861 gtgattttga attcgaaacc tattactcgc tctcttgcca taactgcccg gacgtggtgc
     1921 aggcgctgaa cctgatgagc gtactgaacc cgcgcatcaa gcacactgca attgacggcg
     1981 gcaccttcca gaacgaaatc accgatcgca acgtgatggg cgttccggca gtgttcgtaa
     2041 acgggaaaga gtttggtcag ggccgcatga cgttgactga aatcgttgcc aaaattgata
     2101 cgggcgcgga aaaacgtgcg gcagaagagc tgaacaagcg tgatgcttat gacgtattaa
     2161 tcgtcggttc cggcccggcg ggtgcagcgg cagcaattta ctccgcacgt aaaggcatcc
     2221 gtaccggtct gatgggcgaa cgttttggtg gtcagatcct cgataccgtt gatatcgaaa
     2281 actacatttc tgtaccgaag actgaagggc agaagctggc aggcgcactg aaagttcacg
     2341 ttgatgaata cgacgttgat gtgatcgaca gccagagcgc cagcaaactg atcccagcag
     2401 cagttgaagg tggtctgcat cagattgaaa cagcttctgg cgcggtactg aaagcacgca
     2461 gcattatcgt ggcgaccggt gcaaaatggc gcaacatgaa cgttccgggc gaagatcagt
     2521 atcgcaccaa aggcgtgacc tactgcccgc actgcgacgg cccgctgttt aaaggtaaac
     2581 gcgtagcggt tatcggcggc ggtaactccg gcgtggaagc ggcaattgac ctggcgggta
     2641 tcgttgagca cgtaacgctg ctggaatttg cgccagaaat gaaagccgac caggttctgc
     2701 aggacaaact gcgcagcctg aaaaacgtcg acattattct gaatgcgcaa accacggaag
     2761 tgaaaggcga cggcagcaaa gtcgttggtc tggaatatcg agatcgtgtc agcggcgata
     2821 ttcacaacat cgaactggcc ggtattttcg tgcagattgg tctgctgccg aacaccaact
     2881 ggctcgaagg cgcagtcgaa cgtaaccgca tgggtgagat tatcattgat gcgaaatgcg
     2941 aaaccaacgt caaaggcgta ttcgcagcgg gtgactgtac gacggttccg tacaagcaga
     3001 tcatcatcgc cactggcgaa ggtgccaaag cctctctgag tgcttttgac tacctgattc
     3061 gcaccaaaac tgcataagaa gaagtaagat tcacctgcaa ttgcttagcc gccggggtca
     3121 aacctagcgg cttttttatg gcattaaaaa gccctgccgg gatgttccag gcagggcggc
     3181 ataaatactt ttatctgtta gcgaacgacc agtacaggca gattggcgtg gcggatcacg
     3241 ctcgaggcgt tagaacctaa cagatgggtc gaaatcgatg ggttgcgaga accgataact
     3301 acaacatcag cccccagttc ttctgccaac tcattgactt catcccgcac gctaccaaaa
     3361 cggacatgtt gtttaatgcg ggaaggatcg atggtgaagt ggctgaccat cgtttgcaga
     3421 cgttcttctg cttcatgttg cagatgctct tcaaaacgac gcacatcagc ggcaaaacgg
     3481 tccaggctca ggctggctga cccgggtagt acgtgaagta gatgaataac tccgtcatcc
     3541 tgggtgagga attcagcgtg gcgaacagct ttgtcgctca attccatttc aaatacatca
     3601 actggcataa tgattgtctt atacataacc ctttctccct gttaatcatg aacaaatcat
     3661 tcgccatgat tataatattt atccctgata tttgtctggt tctttttcct tacgaacggt
     3721 ttctgtgatt aatatattct cactgaacac caggaattct cccaaaacct gcggtaccgc
     3781 ccgttttccc gctgtgatag ctacccttaa agactgactc ttttttgaac tgtctctgga
     3841 ggttgcatga aagcattgac ttatcacggc ccacatcacg ttcaggtaga aaatgttccc
     3901 gatccgggca ttgaacaggc agatgatatt attctgcgta ttacggcaac ggcgatctgt
     3961 ggctctgatc tccatcttta tcgaggcaaa ataccccagg ttaaacatgg cgatattttt
     4021 ggtcatgaat ttatggggga agtcgttgaa accggaaagg acgtaaaaaa tttgcaaaaa
     4081 ggcgaccggg tggtaattcc gtttgtcatt gcttgtggcg actgtttttt ctgtcgaatg
     4141 cagcaatatg ccgcctgcga aaataccaat gcgggtaaag gcgctgcgct caataaaaaa
     4201 cagataccag ctcccgcggc attgtttggt tatagtcacc tgtatggtgg cgttcctggt
     4261 ggacaggcgg aatatgtccg cgtccctaaa gggaatgtgg ggccgtttaa agtaccgcct
     4321 ttgctttcag atgataaagc ccttttcctt tctgatattc tgccaacggc atggcaggca
     4381 gcaaaaaatg cgcagatcca acaaggttca agcgttgcag tctatggtgc cggtccagtg
     4441 ggattgttga caatcgcctg tgcacggttg ctcggtgcgg aacagatttt tgttgttgat
     4501 catcatccct accgcttgag tttcgccgct gaccgctacg gcgcgatccc gattaatttc
     4561 gatgaagata gcgatccggc acagtcaatt attgaacaaa cggcaggtca ccggggcgtg
     4621 gatgcagtaa tagacgccgt cggttttgaa gcgaaaggca gcaccacgga aacggtgctg
     4681 actaacctga aactggaggg cagcagcggt aaagcgttgc gccagtgtat tgcggcggtc
     4741 aggcgtggcg gcattgttag cgtaccgggc gtctacgctg gatttattca cggtttcctg
     4801 tttggtgacg cctttgataa agggttgacg tttaaaatgg gacagaccca cgttcacgca
     4861 tggctgggag aattattacc gttaattgag aaaggattac tgaaaccaga agaaattgtt
     4921 acccactata tgccgtttga agaggccgcc cggggatatg agattttcga aaaacgtgaa
     4981 gaggagtgcc gtaaggtgat tctggtaccc ggtgcacaaa gcgcagagga ggcgcagaag
     5041 gcagtttcag gtctggtgaa tgcgatgccg gggggaacaa tatgatcgtc aggagtggtt
     5101 ttcgaggtaa aggacagcca tgacgataat cgccgccata atcagaaatc ctatcaggat
     5161 gtaaaatgct tctgccatgg ttattcccac aaacgaaacg gaataatttt gcagcaaaag
     5221 tgaacagtga gaaccaggaa aaattgctga ttttgcgtaa agaggatgcg agtgcatcct
     5281 ctgggcaaag cgagttatcg cttgtgctga tgggattaaa gcaggtagtc gccagcagct
     5341 tctggctggt attcgagttc cagcacttca aggtgggttg caacgccgcc cggaagttcc
     5401 cagtgaatgg aatcgccaac gcgcagcccc agcagtgcgg cacctaccgg agccataacg
     5461 gaaagctgag tattgctatc ggtcattttt gccggataca ccagcgtgcg cacacgcact
     5521 tcgccatcgc taagattgcg gaatttaacc cggctgttca ttgtcaccac gtcgtgtggc
     5581 atctcttctg gcgaacacat ttgggcgcga tccaactctg cgtttaacgc gtcggcgatt
     5641 ggcaaaccag cataggcggg ttgctccagc agaatatcga tgcgttcggc atccaggtcg
     5701 ttaatgatga tagttggtct ggacattttt actccatgtc gtcggtactg cgagtgtcgc
     5761 agataaacat acccaaaaga aaaccctcac cgtcaggcgg cgagggttta actcacatga
     5821 tgatactgac tgttgctcac tctttgaagt gatttgcgtc acattcaggg aattcctcaa
     5881 tgcagcgcat tatgtataat tcttaatcgt c
//
LOCUS       DAJTMC010000208         5893 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.208, whole genome shotgun sequence.
ACCESSION   DAJTMC010000208 DAJTMC010000000
VERSION     DAJTMC010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5893
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.208"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            <1..1264
                     /locus_tag="OO130_003804"
     CDS             <1..1264
                     /locus_tag="OO130_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCR5367029.1"
                     /translation="ADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQMDGLETVLA
                     AMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVAADVQNPHQPKTATGVIVEALAR
                     RKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAKAVDVKLAQWIEDNVTFPSTMVDRIV
                     PAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAELVSDVLPY
                     EEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRYAAYGLMLQEQAPTLKVQGVD
                     LQDYANRLIERYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKFDLLALGVA
                     GWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIFGDDLPDNS
                     LFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(1300..1503)
                     /gene="ydfZ"
                     /locus_tag="OO130_003805"
     CDS             complement(1300..1503)
                     /gene="ydfZ"
                     /locus_tag="OO130_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HCR5367030.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(1681..2365)
                     /gene="ydfH"
                     /locus_tag="OO130_003806"
                     /pseudo
     CDS             complement(1681..2365)
                     /gene="ydfH"
                     /locus_tag="OO130_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YdfH"
     gene            complement(2454..3200)
                     /gene="ydfG"
                     /locus_tag="OO130_003807"
     CDS             complement(2454..3200)
                     /gene="ydfG"
                     /locus_tag="OO130_003807"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HCR5367031.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWCNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            3337..5382
                     /gene="dcp"
                     /locus_tag="OO130_003808"
     CDS             3337..5382
                     /gene="dcp"
                     /locus_tag="OO130_003808"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HCR5367032.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDANDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLYRQWRGKAPQIMPMLQHRGLNI"
     gene            complement(5427..>5893)
                     /locus_tag="OO130_003809"
     CDS             complement(5427..>5893)
                     /locus_tag="OO130_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HCR5367033.1"
                     /translation="NLTSALSQRLIADQLRLTTAESCTGGKLASALCAAEDTPKFYGA
                     GFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAIERADADVSIAITGYGGLE
                     GGEDGTPAGTVWFAWHIKGQTYTAVMHFAGDCETVLALAVRFAHVQLLQLLL"
BASE COUNT         1458 a         1425 c         1529 g         1481 t
ORIGIN      
        1 tgccgattta caacagcaag ataatcttta taccgttgcg gaaatgtcgg ccgatgcgtg
       61 gacggctcgc gtcgttggcg tcgttaaaaa agccttgcac gtacagatgg atggcttaga
      121 aaccgtgttg gctgcgatgt gtgaaccgca aatcgcgatt gtctctctga caatcaccga
      181 aaaagggtat ttccactctc cggcgaccgg acagttaatg ctcgatcacc cgatggtcgc
      241 tgccgacgtg caaaatcccc accagccgaa aacagcaaca ggggtgattg ttgaggcgct
      301 ggcccgccgt aaagcggcag gacttcccgc atttaccgtc atgtcatgtg acaacatgcc
      361 agaaaacggt catgttatgc gtgacgttgt cacttcatac gcgaaagctg ttgatgtaaa
      421 actggcacaa tggatcgaag ataacgtgac tttcccatca acaatggtgg accgtattgt
      481 gcccgcagtg acagaggata cgctggcgaa aatcgaacaa cttaccggtg tgcgcgatcc
      541 tgcgggcgtt gcctgtgaac ctttccgcca gtgggtaata gaagataact ttgttgccgg
      601 acgtccagaa tgggaaaaag cgggagccga actggttagt gatgtgctgc cttatgaaga
      661 gatgaagttg cgcatgctca acggcagtca ttcattcctg gcgtatttgg gttatcttgc
      721 cggatatcag cacattaatg actgcatgga agatgaacat tatcgttatg cggcgtatgg
      781 cttgatgttg caggaacaag cgccgacgct gaaagtgcag ggcgttgatt tgcaagatta
      841 cgctaaccga ttaattgaac gctatagcaa tccggcgtta cgtcatcgaa cctggcagat
      901 tgcgatggat ggtagccaga aattgccaca gcggatgttg gattctgttc gctggcatct
      961 ggcgcatgac agcaagttcg atctgctggc gctgggcgtc gcgggttgga tgcgttatgt
     1021 cggtggtgtt gatgaacagg gaaatccgat agaaatcagt gacccactgt tacctgttat
     1081 tcagaaggct gtacaaagta gtgccgaagg gaaagcgcgc gtccagtcat tgctggcgat
     1141 taaggcaatc tttggtgatg atttgccaga caatagcttg tttactgcaa aagtgacgga
     1201 agcgtacttg tctttattag cgcatggcgc gaaagcgacc gtggcgaaat attccgtgaa
     1261 gtaaaaaaag caatgccgcc atcggcggca ttcacacact tagttcatgc cgagacgaat
     1321 caggtccaga ggtgccagtt tctcttcaca accttcaaca actacggttt ttccgcggcg
     1381 gatttgctca gcggtcagac cttcagtatc aatcgacagg atcttgccag tgtgaccggt
     1441 gccgctaacc ataacacggc tgccagtggt gattgcgtta cggttacgat cgtaggtcgt
     1501 cataggtgtt ttctcctttc tgatttacag tcgaagccca cgtaacgggc gcaatataaa
     1561 tactcctgtc gagtgaaata ttttttgttt ttgatcaagc tcacatcttt tttgatgcat
     1621 gcatgagccg taaggcgggg ggaaaagcct ctcatcccgg atgggatgag agtaaatgaa
     1681 ttactcttcg ctaaaccagt cgctgttttc ctggcggatc tggccacgga ttcgctgatt
     1741 tcctgcaaat gttgcgtcat tgcacgttct accgcatcgc catcacgttt ttgcagggca
     1801 gagaaaatat caagatgctg gcgtaacagc atttctggtg gagaaacatg gtcgaaactc
     1861 atatagcgca cgcgatcaac ggtcgctttc aggttctcaa tggtatccca cgccagttga
     1921 cagtcggcaa tctgcgtcag gagttgatgg aagttgtcat caagttcaaa aaatcatcca
     1981 gttgcttgtg ctcaatggca atgcgttgct ggtgaagatt ttgttccagt tggtagcact
     2041 ggctttcggt aatcatgctc gccgcccgac gcgccaccgc gcactcaatg gcctgacgga
     2101 taaaactgcc gttgcgcacc tgggccatgg aaattttgtt gacgtagctg ccacgctgtg
     2161 gacgaatttg aatcaggccg ttttccgcca gtttaataaa ggcttcacga accggctggc
     2221 gtgacacatt gaaacgaaca gaaacttctt tttccgacaa cggtgtgcct ggagcaatca
     2281 ggcaatggac aatgtcgcga cgaaggatac gataaatttg ctgattgaca ggctgtgtgg
     2341 gattaagttg cgtttcgacg gtcattcgtt cttgcttgtg agtgagttaa ctgcatgagt
     2401 ctaccacttt tttgtgacaa gcaataaccc ggcagttacg ccgggtataa aaattactga
     2461 cggtggacat tcagtccggc atagctttgg gtaaccggca tcatttccag ggtattgata
     2521 ttgacgtgag caggcagcgt tgacacccac caaacggctt cgctgacatc ttctggcgtc
     2581 aatgcaacgg tattttgata ggttttttcc gctttaccgt catcgccttt aaagcggaca
     2641 ttggaaaact cggtgccacc caccagaccc ggttcgatgt cggtgacgcg caccgccgta
     2701 ccatgcaggt ccgtacgcag attcaggcta aactgacgaa caaacgcttt cgtcgcaccg
     2761 taaacgttac caccggcata aggccagcta cctgccgttg agccaatgtt aataatatga
     2821 ccatgattac gttcaaccat acccggtaag acggcgcgcg tcatatatac caggcctttg
     2881 ttgttggtat caatcatcgt ttcccagtct tcaacgctgg ctttatgcgc aggctccatg
     2941 cccaacgcca ggccggcatt atttaccagg atatcaatat tgcaccactc ggcaggaagc
     3001 gatgccagca tctcttcaat agcggcgcgg ttgcgaacgt ccagttgggc gatgtacaga
     3061 ttatctccca gttcgtcttt taactcctgc aaccgttcct ggcgacggcc agtggcgata
     3121 actttatgcc cttgttgaat aaaacgacga gtaatgcatt caccaaaacc tgccgttgct
     3181 ccagttacta aaacgatcat ctcactgttc ctcaacgctt ttgtgtgtca ataacatagc
     3241 acgtgatgct taagagcggg taatactggt ttgtaagatt gcactccgtt atcagtaagc
     3301 ctacgctatg gaacacctcg cgttcaggag aagaaaatga caacaatgaa tcctttcctt
     3361 gtgcaaagca cactaccgta tctggctccc cattttgatc aaattgccaa tcatcactat
     3421 cgcccggcat tcgatgaggg aatgcagcaa aagcgggcag aaattgctgc catcgcgctt
     3481 aacccgcaaa cgcctgattt caacaatact attctggcac tggaacaaag cggagaatta
     3541 cttacccgcg ttaccagcgt cttttttgcg atgactgcgg cgcataccaa tgatgaatta
     3601 cagcgtcttg atgaacagtt ttccgctgaa ctggcggaac tggctaatga tatctatctg
     3661 aacggtgaat tattcgcgcg ggtagatgct gtctggcagc gccgtgaatc cctggggctt
     3721 gatagtgaat ccatccgcct ggtggaggtg attcatcaac gttttgtcct tgccggagcc
     3781 aaacttgcgc aagctgataa agcaaaatta aaagtactga atacagaagc tgcgaccctg
     3841 accagccagt ttaaccagcg attactggca gcaaataaat ccggcggtct ggttgtgaac
     3901 gatatcgcgc agctggcagg aatgagtgag caagagattg cgctggcggc agaggcggct
     3961 cgcgagaaag gtctggataa caaatggctg attccgctgc tgaataccac ccaacaaccg
     4021 gcgcttgccg agatgcgcga tcgtgcgacg cgtgaaaaac tgtttattgc gggctggacg
     4081 cgagcggaaa aaaatgatgc caatgatacc cgcgctatca ttcaacgtct ggtagagatt
     4141 cgcgcacagc aggcgaaact gcttggtttt cctcattatg ccgcatggaa aatcgccgat
     4201 cagatggcaa aaacgccaga agcagcactc aactttatgc gggaaattgt tccagcggcg
     4261 cgtcaacgtg ctagcgatga attagcctcc atacaggcgg ttatcgataa gcagcaaggc
     4321 gggtttagcg cgcagccgtg ggactgggca ttttatgccg aacaggtacg gcgggagaaa
     4381 tttgatcttg atgaggcgca gctcaagcca tattttgaat taaacacggt gttgaatgaa
     4441 ggtgtattct ggaccgcgaa tcagctcttc ggtattaagt ttgtcgaacg ttttgatatt
     4501 cctgtctacc atcctgacgt tcgtgtgtgg gaaatttttg atcataatgg cgtgggactg
     4561 gcgttatttt acggtgattt cttcgcccgt gattcaaaaa gcggcggtgc atggatgggc
     4621 aattttgttg agcaatcaac gcttaatgaa acgcatccgg taatttataa cgtttgcaat
     4681 tatcagaaac ccgctgccgg tgagcctgcg ttgttactct gggatgatgt cataacctta
     4741 ttccatgaat ttggtcatac gctgcacggc ctttttgccc gccagcgtta tgccacgctt
     4801 tccggcacca acacgccgcg tgattttgtc gaatttccgt cgcaaatcaa cgaacactgg
     4861 gcaacgcatc cgcaggtatt cgctcgctac gcccggcatt atcagagcgg ggcagcaatg
     4921 cctgacgaac tgcaacagaa aatgcgtaat gccagcctgt tcaacaaagg gtatgagatg
     4981 agcgaactgc ttagcgccgc acttctcgat atgcgctggc attgcctgga agaaaacgaa
     5041 gcaatgcagg atgtcgatga ttttgaattg cgggcgctgg tggcggaaaa tatggatctt
     5101 cctgctatac cgccacgcta tcgcagcagt tatttcgccc atatttttgg tggcggatat
     5161 gctgcaggtt attacgctta tctgtggacg caaatgttgg ccgatgatgg ttaccagtgg
     5221 tttgttgagc agggcggatt aacgcgtgaa aatgggcagc gttttcgcga ggcgatcctt
     5281 tccagaggta acagcgaaga tctggaacgc ctgtatcgac aatggcgcgg taaggctcct
     5341 cagattatgc cgatgctgca acatcgtggc ttgaatatat aaacaccacc aatggctggc
     5401 ctggagatat cgccaggcca gcctggttat agcagtaatt gcagcagctg gacgtgggca
     5461 aacctcaccg ctaaagctaa taccgtttcg cagtcgccag caaaatgcat aaccgcagtg
     5521 taggtctggc ctttaatatg ccacgcgaac cagacggtac ccgctggcgt accatcttca
     5581 ccgccctcca gtccgccgta gccggtaatg gcaatactga catcagcatc cgcacgctct
     5641 atggcacctg ttgccatttc tgctgccact ttctcactca ccgcagaata tcgttcaaga
     5701 gattgctggc ttacgctgag gattttcatc tttgcctgat cggtgaaagt aacaaagcct
     5761 gcaccgtaaa atttgggtgt atcttcagct gcacacaggg cgctagccaa cttaccgccg
     5821 gtgcatgatt cggcggtagt taagcgtaat tgatccgcga taagtctttg actcaacgcg
     5881 gatgtcaggt ttt
//
LOCUS       DAJTMC010000209         5812 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.209, whole genome shotgun sequence.
ACCESSION   DAJTMC010000209 DAJTMC010000000
VERSION     DAJTMC010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5812
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.209"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            <1..853
                     /gene="pdeC"
                     /locus_tag="OO130_003810"
     CDS             <1..853
                     /gene="pdeC"
                     /locus_tag="OO130_003810"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HCR5367034.1"
                     /translation="ALPAGILGSLVLLLLWLRIRQNYLSPKRKLQRALEKHQLCLYYQ
                     PIIDIKTEKCIGAEALLRWPSEQEQVMNPAEFIPLAEKEGMIEQVTNYVIDNVFRDLG
                     DYLATHADRYVSINLSASDFHTSRLIARINQKTEQYAVRPQQIKFEVTEHAFLDVEKM
                     TPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVETLTTHKTSHLIAE
                     HIIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMPPQVFMQWMEQLPA
                     RELTRGQ"
     gene            complement(856..1179)
                     /gene="soxS"
                     /locus_tag="OO130_003811"
     CDS             complement(856..1179)
                     /gene="soxS"
                     /locus_tag="OO130_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HCR5367035.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            1265..1729
                     /gene="soxR"
                     /locus_tag="OO130_003812"
     CDS             1265..1729
                     /gene="soxR"
                     /locus_tag="OO130_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HCR5367036.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYRRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            2275..3624
                     /gene="ghxP"
                     /locus_tag="OO130_003813"
                     /gene_synonym="yjcD"
     CDS             2275..3624
                     /gene="ghxP"
                     /locus_tag="OO130_003813"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HCR5367037.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFGMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            3776..5425
                     /locus_tag="OO130_003814"
     CDS             3776..5425
                     /locus_tag="OO130_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HCR5367038.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(5579..>5812)
                     /locus_tag="OO130_003815"
     CDS             complement(5579..>5812)
                     /locus_tag="OO130_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCR5367039.1"
                     /translation="MFNTRLYRVNFDEASVQGMGISILRGEENIPIDSDTLVTLQKFF
                     EEDCTSHTGMSQTEDNINAVAMKITADIMQHAD"
BASE COUNT         1258 a         1376 c         1602 g         1576 t
ORIGIN      
        1 tgcgttgccc gccggtattt tggggagtct tgttctgcta ttactctggc tacgtattcg
       61 acaaaactat ttgtctccca aacgcaaatt gcaacgcgct ctagaaaaac atcaactttg
      121 tctttattac cagccaataa tcgatatcaa aacagaaaaa tgtatcggcg ctgaagcgtt
      181 gttacgttgg cctagtgagc aggagcaagt aatgaatccg gcagagttta ttccgctggc
      241 agaaaaggag gggatgatag aacaggtaac taattatgtt attgataatg tcttccgcga
      301 tctgggcgat tacctggcaa cacatgcaga tcgctatgtt tctattaacc tgtcggcctc
      361 cgattttcat acgtcacggt tgatagcgcg aatcaatcag aaaacagagc aatacgcggt
      421 gcgtccgcag caaattaaat ttgaagtgac tgagcatgca tttcttgatg tcgaaaaaat
      481 gacgccgatt attctggctt tccgccaggc tggttacgaa gtggcaattg atgattttgg
      541 tattggctac tctaacttgc ataaccttaa atcattgaat gtcgatattt tgaaaatcga
      601 taaatcgttt gttgaaacgc tgaccaccca caaaaccagt catttgattg cggaacacat
      661 catcgagctg gcgcacagcc tggggttaaa aacgatcgct gaaggcgtcg aaactgagga
      721 gcaggttaac tggctgcgca aacgcggcgt gcgctattgc cagggatggt tttttgcgaa
      781 ggcgatgccg ccgcaggtgt ttatgcaatg gatggagcaa ttacccgcgc gggagttaac
      841 gcgcgggcaa taaaactaca ggcggtggcg ataatcgctg ggagtgcgat caaactgccg
      901 acggaaaacg cgggagaagg tctgctgcga gacataaccc aggtccattg cgatatcaaa
      961 aatcggacgc tcggtggtgc gcaactcaac ggcggccagt aacaggcgac gttggcgaat
     1021 gtaatcgcca agcgtctgat gcgtcaccgt gcggaacatt cgttgcaagt accactttga
     1081 atagcctgat ttttttgcga ctacatcaat gttaagcggc tggtcaatat gctcgtcaat
     1141 ccatgcgata agatcctgaa taattttctg atgggacata aatctgcctc ttttcagtgt
     1201 tcagttcgtt aattcatctg ttggggagta taattcctca agttaacttg aggtaaagcg
     1261 atttatggaa aagaaattac cccgcattaa agcgctgcta acccccggcg aagtggcgaa
     1321 acgcagcggt gtggcggtat cggcgctgca tttctatgaa agtaaagggt tgattaccag
     1381 tatccgtaac agcggcaatc agcggcgata tagacgtgat gtgttgcgat atgttgcaat
     1441 tatcaaaatt gctcagcgta ttggcattcc gctggcgacc attggtgaag cgtttggcgt
     1501 gttgccagaa gggcatacgt taagtgcgaa agagtggaaa cagctttcgt cccaatggcg
     1561 agaagagttg gatcggcgca ttcatacctt agtggcgctg cgtgacgaac tggacggatg
     1621 tattggttgt ggctgccttt cgcgcagtga ttgcccgttg cgtaacccgg gcgaccgctt
     1681 aggagaagaa ggtaccggcg cacgcttgct ggaagatgaa caaaactaaa gcgccacaag
     1741 ggcgctttag tttgttttcc ggtctttgtc tttctctcta tcccgctggt acacaggagg
     1801 gtttcccccg acgtcaacac acctcattcg agcacgtggt ggaggttccg gttggtgttg
     1861 atgctttaat tgtatgtcac cgacgtttct tcgccagtgt aaaagtatac tttttaaccg
     1921 caatattttt gtcatctcag acgatttttt atcgcaatcc tgaacggtat acggctcgat
     1981 aacgctgcaa tcttgcgcac cgacgataac gtttgcgcat caattgcctg ttttttcatc
     2041 gtcaagacaa taaaagagaa aaaagcagca aacttcggtt gaaaaagccg ctatgatcgc
     2101 cggataatcg tttgcttttt ttaccacccg ttttgtatgc gcggagctaa acgtttgctt
     2161 ttttgcgacg cagtaaattg tcgcaaacct ggagcaggaa gataacgttt cgctggcagg
     2221 ggattgtccg ccacgcatct tgacgaaaat taaactctca ggggatgttt tcttatgtct
     2281 acgccatcag cgcgtaccgg cggttcactc gacgcctggt ttaaaatttc acaacgtgga
     2341 agcactgtcc gtcaggaagt ggttgccggg ttaacaacgt ttctggcgat ggtctactcg
     2401 gtcatcgtcg ttccaggtat gttgggtaaa gcaggcttcc cgcctgcggc agttttcgtt
     2461 gcaacctgtc tggttgccgg actcggttct atcgtgatgg gtctgtgggc taatctgccg
     2521 ttggcgattg gttgcgccat ctccctgacg gcgtttaccg cattcagcct ggtgctgggg
     2581 caacatatta gcgtacctgt cgcgctgggt gccgtgttcc tgatgggtgt gctgtttacg
     2641 gtaatttctg ccacgggtat ccgtagctgg attttgcgca acttgccgca cggtgtggcg
     2701 cacggcacgg ggatcggtat cggtctgttc ctgctgctca ttgccgctaa tggtgttggt
     2761 ctggtgatta aaaacccgct tgatggtctg cccgttgcgc tgggtgattt cgcgaccttc
     2821 ccggtgatta tgtcactggt aggtctggcg gtgatcatcg gcctggaaaa actgaaagtc
     2881 cctggtggca ttctgctgac cattatcggt atctcaattg tcggtttgat cttcgatcct
     2941 aacgtccatt tctccggcgt tttcggcatg ccttcattga gcgatgaaaa cggcaattca
     3001 ctgattggca gcctggacat tatgggcgcg ctgaatcctg tagtcctgcc aagcgttctg
     3061 gcgctggtga tgacggcagt atttgatgcc accggaacta tccgtgccgt cgccggccag
     3121 gcgaacctgc tggataaaga tgggcagatc atcgacggtg ggaaagcact gaccactgac
     3181 tccatgagca gcgttttctc tggcctggtg ggtgctgctc cggcagcggt atacatcgag
     3241 tctgcggcgg gtacggcggc gggcggtaaa accggtttga cggctatcac cgttggcgtg
     3301 ctgttcctgc tgattctgtt cctctctccg ctctcttacc tcgttccggg gtatgcaacg
     3361 gctccggcgc tgatgtacgt tggcctgctg atgctgagca acgtggcgaa aatcgacttt
     3421 gctgattttg ttgatgcgat ggcgggtctg gttacggcgg tattcatcgt gctgacctgt
     3481 aacatcgtaa caggcatcat gatcggcttc gcgactctgg tgattggtcg tctggtttcc
     3541 ggcgaatggc gcaagttgaa catcggtacg gtcgttatcg ccgtggcgct ggtgaccttc
     3601 tatgcgggtg gctgggctat ctaatctttc atcggattct gaaaacgggt ggcaatggct
     3661 gcccgttttt attttctccg cacattgtgt gagcttttgc gatattctga aaaaatgaga
     3721 attcaggcat aacgcctttc tcagatcata aaaaaacatc gcaaacaggg aacgcatgga
     3781 aatcttcttc accatactga taatgaccct cgtggtctcg ctgtccgggg tggtcactcg
     3841 tgtcatgccc tttcagatcc cgcttccgct tatgcaaatc gccatcggtg cgctactggc
     3901 gtggccgacg tttggtttgc atgtggagtt tgatcctgaa ctctttttag tcttgtttat
     3961 cccgccgttg ctgttcgctg atggctggaa aacgccgacc cgtgaatttc tcgaacatgg
     4021 tcgagagatt ttcggcctcg cgctggcgct ggtggtggtc accgtggtcg gcattggctt
     4081 ccttatttac tgggtggtgc cgggcattcc gctgatcccc gcctttgcgc tggcggcggt
     4141 actttctccg accgatgctg tggcgctctc cggaattgtt ggcgaagggc gcatcccgaa
     4201 aaaaatcatg ggcattttgc agggcgaagc gttgatgaat gacgcctccg gcctggtgtc
     4261 gttgaagttt gccgtggcag tggcgatggg gacgatgatc ttcaccgtcg gcggtgcgac
     4321 ggtcgaattt atgaaagtag ccattggtgg tattcttgcc ggttttgtgg tgagctggct
     4381 gtatggtcgc tcgctgcgat tcctcagccg ctggggcggt gatgaacccg cgacgcagat
     4441 cgttctgctg ttcttgctgc cattcgcttc ttatctgatt gccgaacata ttggcgtttc
     4501 cggcatcctc gctgcggttg ccgccgggat gaccatcacc cgctccggtg tgatgcgccg
     4561 tgcgccgctg gcaatgcgcc tgcgcgcaaa cagcacctgg gcgatgctgg aatttgtctt
     4621 taacggcatg gtgttcctgc tgttaggtct gcagctgccg ggtattctgg agacgtcgct
     4681 gatggcggca gaaatcgacc ctaacgtcga aatctggatg ctgtttaccg atattattct
     4741 gatatatgcg gcgctgatgc tggtccgttt cggctggctg tggacgatga aaaagttcag
     4801 caaccgcttc ctgaagaaga agccgatgga gtttggttcg tggaccacac gagaaatcct
     4861 gatcgcgtct ttcgccgggg tgcgtggggc gatcactctg gccggtgtgc tctctatccc
     4921 gctgctcttg ccggatggta acgtcttccc ggcgcgctat gagctggtgt tcctggcggc
     4981 tggcgtcatt ctcttctcgc tgtttgtcgg tgtggtgatg ttgcctattc tgctacaaca
     5041 cattgaagtc gcggatcatt cgcaacaatt gaaagaggaa cgtattgcgc gagcggcaac
     5101 ggcagaagtg gcgattgtgg cgatccagaa aatggaggag cgtctggcgg cggataccga
     5161 agagaatatc gataaccagc tgcttacgga ggtcagttct cgcgtcattg gtaacctgcg
     5221 tcgtcgcgcc gatggacgta acgacgttga aagttccgtg caggaagaga accttgagcg
     5281 tcgcttccgt ctggcggcat tgcgttctga acgtgctgaa ctgtaccacc tgcgcgccac
     5341 gcgggagatc agcaacgaaa cgctgcaaaa attactgcac gatctcgatt tgcttgaagc
     5401 gttgctaatt gaggaaaatc agtaagccga aagcgacgta accaggcccg aggtaatctg
     5461 aaaaaggtgc aatcttttgc acctttaaca ggttagctcg ggctaacatt catgattcta
     5521 aaacaaataa gttatctctc ttgtaaagtc aattcattgg ctgttcttcg acttaacctc
     5581 aatctgcgtg ttgcataata tctgcagtaa tcttcatagc gactgcatta atattatcct
     5641 cagtttgtga catgccagta tgagaggtac aatcttcttc aaagaatttc tgtagtgtta
     5701 ccagggtatc actatcaatg gggatatttt cctctccacg gagtatggaa atgcccattc
     5761 cctggacgct agcctcatca aaattaaccc gatagagacg ggtattaaac at
//
LOCUS       DAJTMC010000210         5745 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.210, whole genome shotgun sequence.
ACCESSION   DAJTMC010000210 DAJTMC010000000
VERSION     DAJTMC010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5745
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.210"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(1..383)
                     /locus_tag="OO130_003816"
                     /pseudo
     CDS             complement(1..383)
                     /locus_tag="OO130_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151035092.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            complement(569..3877)
                     /gene="icsA"
                     /locus_tag="OO130_003817"
                     /gene_synonym="virG"
     CDS             complement(569..3877)
                     /gene="icsA"
                     /locus_tag="OO130_003817"
                     /gene_synonym="virG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane autotransporter IcsA"
                     /protein_id="HCR5367040.1"
                     /translation="MNQIHKFFCNMTQCSQGGAGELPTVKEKTCKLSFSPFVVGASLL
                     LGGPIAFAIPLSGTQELHFSEDNYEKLLTPVDGLSPLGAGEDGMDAWYITSSNPSHAS
                     RTKLRINSDIMISAGHGGAGDNNDGNSCGGNGGDSITGSDLSIINQGMILGGSGGSGA
                     DHNGDGGEAVTGDNLFIINGEIISGGHGGDSYSDSDGGNGGDAVTGVNLPIINKGTIS
                     GGNGGNNYGEGDGGNGGDAITGSSLSVINKGTFAGGNGGAAYGYGYDGYGGNAITGDN
                     LSIINNGAILGGNGGHWGDAINGSNMTIANSGYIISGKEDDGTQNVAGNAIHITGGNN
                     SLILHEGSVITGDVQVNNSSILKIINNDYTGTTPTIEGDLCAGDCTTVSLSGNKFTVS
                     GDVSFGENSSLNLAGISSLEASGNMSFGNNVKVEAIINNWAQKDYKLLSADKGITGFS
                     VSNISIINPLLTTGAIDYTKSYISDQNKLIYGLSWNDTDGDSHGEFNLKENAELTVST
                     ILADNLSHHNINSWDGKSLTKSGEGTLILAEKNTYSGFTNINAGILKMGTVEAMTRTA
                     GVIVNKGATLNFSGMNQTVNTLLNSGTVLINNINAPFLPDPVIVTGNMTLEKNGHVIL
                     NNSSSNVGQTYVQKGNWHGKGGILSLGAVLGNDNSKTDRLEITGHASGITYVAVTNEG
                     GSGDKTLEGVQIISTDSSDKNAFIQKGRIVAGSYDYRLKQGTVSGLNTNKWYLTSQMD
                     NQESKQMSNQESTQMSSRRASSQLVSSLNLGEGSIHTWRPEAGSYIANLIAMNTMFSP
                     SLYDRHGSTIVDPTTGQLSETTMWIRTVGGHNEHNLADRQLKTTANRMVYQIGGDILK
                     TNFTDHDGLHVGIMGAYGYQDSKTHNKYTSYSSRGTVSGYTAGLYSSWFQDEKERTGL
                     YMDAWLQYGWFNNTVKGDGLTGEKYSSKGITGALEAGYIYPTIRWTTHNNIDNALYLN
                     PQVQITRHGVKANDYIEHNGTMVTSSGVNNIQAKLGLRTSLISHSCIDKETLRKFEPF
                     LEVNWKWSSKQYGVIMNGMSNHQIGNRNVIELKTGVGGRLADNLSIWGNVSQQLGNNS
                     YRDTQGILGVKYTF"
     gene            4406..5608
                     /gene="virA"
                     /locus_tag="OO130_003818"
     CDS             4406..5608
                     /gene="virA"
                     /locus_tag="OO130_003818"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-tubulin-specific protease"
                     /protein_id="HCR5367041.1"
                     /translation="MQTSNITNHERNDSSWMSTVKSTTEVSWNKLSFCDVLLKIITFG
                     IYSPHETLAEKYSEKKLMDSFSPSLSQDKMDGEFAHANIDGISIRLCLNKGICSVFYL
                     DGDKIQSTQLSSKEYNNLLSSLPPKQFNLGKVHTITAPVSGNFKTHKPAPEVIETAIN
                     CCTSIIPNDDYFPVKDTDFNSVWHDIYRDIRASDSNSTKIYFNNIEIPLKLIADLINE
                     LGINEFIDSKKELQMLSYNQLNKIINSNFPQQDLCFQTEKLLFTSLFQDPAFISALTS
                     AFWQSLHITSSSVEHIYAQIMSENIENRLNFMPEQRVINNCGHIIKINAVVPKNDTAI
                     SASGGRAYEVSSSILPSHITCNGVGINKIETSYLVHAGTLPSSEGLRNAIPPESRQVS
                     FAIISPDV"
BASE COUNT         1777 a         1299 c          949 g         1720 t
ORIGIN      
        1 cgcttcccca tgtgatttgc ctcgccactt caccgggctc aggaccccgt aggatcttca
       61 ccgaaaaaaa tgcatcacca gttgctttat tatttgtttt aggtagacct gttactaatt
      121 tatacctact ctgaccaagt ctggataaga aaggcacacc tgcacatttc tacttggaaa
      181 tgttttcttt taccattttg ctcaaaaatt tcaacatcca atattcctcg tacagaacta
      241 ctcaagtcct gaatagaaaa agttccagct ggtacagtag agtcatatat tgctcgacct
      301 tgttgagata caacaacacg tgcattagtt tctgcaatac cagtaatctg aggcgcataa
      361 ccacgaatat ttggcggtag cattcgttca tcgctttcta atgcaattcc tgtgtaacgc
      421 caagcaacag cgcattctgg cgcatgatct ggtaaacacg tttggcattg atcgcaggca
      481 taccatcatg tgcacaaaaa cccaccgagc ttttacacgg tgggtcccag agaaatgcag
      541 gacatcaaca cgccctgcat ttttattatc agaaggtata tttcacaccc aaaatacctt
      601 gggtgtctct gtaactgtta ttacccaatt gctgagatac gtttccccag atgcttaggt
      661 tatctgcaag acgccccccc acaccagttt tgagttcaat cacattacgg ttgcctatct
      721 ggtgatttga catgccattc ataattacac catattgctt tgagctccat ttccaattca
      781 cttccaaaaa tggttcgaac ttacgaagag tctccttatc gatacaacta tgactaatta
      841 aggatgtacg caatcccaat tttgcttgaa tattattgac cccagaggat gtgaccatag
      901 tgccattgtg ttcaatatag tcgtttgctt ttaccccatg cctagttatc tggacttgtg
      961 gattgagata caatgcgttg tcaatattat tatgagtagt ccagcgtatg gttgggtaga
     1021 tatagccagc ttccaaagct cctgttattc ctttgctgga atatttctca ccagttaacc
     1081 catctccttt gactgtatta ttaaaccaac cgtactgcaa ccaagcatcc atatatagac
     1141 ctgttcgttc tttttcatcc tgaaaccaag aactgtacaa accggcagta taaccgctca
     1201 cagttcctcg tgaactataa ctagtatact tattatgagt tttgctatcc tgatatccat
     1261 aagctcccat aatacccaca tgcaagccat catgatcagt gaagtttgtc ttcaaaatat
     1321 ctccaccaat ctgataaacc atcctgttag ctgtggtttt taattgtcta tcagctaaat
     1381 tatgctcatt atgtccacca acagtacgaa tccacatggt ggtttcgctg agctgacctg
     1441 tagtaggatc aacaatagtg ctaccgtgtc ggtcatagag agaaggacta aacatcgtgt
     1501 tcattgctat caggttagca atataactgc cagcttcagg gcgccatgtg tgaatactac
     1561 cttcacccaa attaagtgaa gatacaagct gtgaactagc tcggcgacta ctcatttgag
     1621 tagactcttg attgctcatc tgttttgatt cttgattatc catctgacta gttagatacc
     1681 acttattggt attcagtcca gatacagtgc cctgtttcag gcgatagtca taactaccag
     1741 caacaatacg gcctttctga ataaaagcat tcttatcaga agaatctgtc gaaataattt
     1801 gaacaccttc aagagtttta tctccagagc ctccctcatt tgtcactgca acataggtaa
     1861 taccagacgc atggcctgta atttccagcc ggtcagtttt actgttgtca ttgccgagaa
     1921 ccgcgcccaa agataatatt ccgccctttc catgccaatt acctttctga acataggtct
     1981 gaccgacatt tgacgaacta ttattgagaa taacatgacc gtttttctcc agagtcatgt
     2041 tacctgtgac aatgacgggg tcaggcaaaa aaggggcatt aatattattg attagcacag
     2101 tcccactatt taataaagtg ttaacagttt ggttcatgcc tgaaaaattc aaggtagcac
     2161 ctttattaac aataacacca gcggtacgtg tcatagcttc aactgtcccc atttttagaa
     2221 tgcctgcatt gatgttggtg aaaccagagt aggtattttt ttccgccaaa atgagagttc
     2281 cctcccctga ttttgttagg gattttccgt cccagctatt tatattatga tggctgagat
     2341 tatctgccag aatagtacta acagtaagtt cagcgttttc tttcagattg aactctccat
     2401 gactgtcgcc atctgtatca ttccagctca aaccgtagat caatttattc tggtcactga
     2461 tatagctttt tgtatagtca atagcaccag tagtgagtaa cggattgatg atagatatat
     2521 tagaaacact gaaacctgtt atccctttat ctgcacttag cagtttatag tccttctgcg
     2581 cccagttatt tataatagct tccactttta cattgttgcc aaatgacata tttccagaag
     2641 cttccagact actgattcca gctaaattta aagaactgtt ctcaccaaaa gaaacgtcac
     2701 ctgaaacagt gaatttgtta cctgatagtg aaacagttgt acaatcacca gcacataaat
     2761 caccttcaat agttggtgtg gtcccagtgt aatcattgtt gataattttc agaatggatg
     2821 aattgttaac ctgtacatca ccagtaatga cagaaccttc atggagtatt aatgaattgt
     2881 ttccaccagt gatgtggata gcattacctg ctacattttg tgttccatca tcttctttac
     2941 ctgaaattat atatccgcta ttagcaatgg tcatattgct accatttata gcatcccccc
     3001 aatgtccacc attaccgcct aaaatagctc cattgttgat tatagacagg ttatctcctg
     3061 tgatagcatt accaccgtag ccatcataac cataaccgta agcagcacct ccgttgcctc
     3121 cagcgaacgt gcccttattg attacagaga ggctgcttcc tgtgatggca tcacctccat
     3181 taccgccatc accctcacca taattgttac ctccattacc accggaaata gtccctttgt
     3241 tgattatggg tagattgact cctgtgacgg catcacctcc attcccccca tcactatcac
     3301 tataactatc gccaccatgt ccacctgaaa taatttctcc atttattata aacagattgt
     3361 ctcctgtaac agcctcacca ccatcaccgt tatggtcagc accgctaccg ccgctaccac
     3421 caagaatcat gccttgattg attatagaca agtcagatcc ggtaatagag tcaccaccat
     3481 taccgccaca actattacca tcattattat caccagcacc accatgacct gcgctaatca
     3541 taatatcaga gttaatccgt agcttagttc tagatgcatg agaggggttg gaagaagtta
     3601 tataccacgc atccattcca tcttcaccag ctcccaaggg agaaagtcca tcaacaggtg
     3661 ttaataattt ttcataattg tcctctgaaa aatgaagttc ttgagtaccc gaaagaggaa
     3721 tagcaaaagc tattggcccc ccgagcaaca gggatgcacc aacaacaaaa ggagaaaaag
     3781 acaatttgca tgttttttcc tttaccgtag gtaattctcc ggcccccccc tgtgaacatt
     3841 gggtcatatt acaaaaaaat ttgtgaattt gattcatgca ctatattatc agtaagtggt
     3901 tgataaaccc ctaaaaagat tccgttttat ccggaataaa gggacgatat atgcaaaaac
     3961 atattaaaca aagcctcaac caaaacatga gagctgtgtt atatatcaat gcaacgggag
     4021 aaatttacct gtgtcttgct ttgaaaaaaa gagggggaaa gctatatgtt cgataataaa
     4081 ttaataattt tactatgata gtctgcttca gaccgcaact tttcagttct ttctgtacgc
     4141 ttgcccaaag tttaaccact ttgtttttct atacacccaa tggttctatc tgccccttga
     4201 tgttaatccc caaagccaat aacatgaatg tattcatatc acaactttcc taccagactc
     4261 tccaatgcaa taactgcata attgttttta gtggaaatgt atcgttttct taaagagaag
     4321 aataacattc cattttatta tgttcccaat ttaattatat taatatatat tggcatcaat
     4381 aggaaaatac atcaggagaa atcaaatgca gacatcaaac ataactaacc atgaaagaaa
     4441 tgactcatca tggatgtcaa ctgtcaaatc aactacagaa gtaagttgga ataaattgag
     4501 tttttgtgac gttttactga aaataataac tttcggaata tattctccac acgaaactct
     4561 agccgaaaaa tatagcgaaa aaaaactaat ggatagtttc tcaccatcac tatcacaaga
     4621 taaaatggac ggagagtttg cccacgcaaa catagatggc atttcaatac ggctatgcct
     4681 taacaaaggc atatgttcgg ttttttatct tgatggagat aaaatacaat ccactcaact
     4741 ttcatcaaaa gaatataata acttgttaag ttcactcccc cccaaacaat tcaacttagg
     4801 aaaagtacat actattacag ctccggtatc agggaatttt aaaactcaca agcctgcacc
     4861 agaagttatt gaaacagcca ttaattgctg tacatcgata ataccaaacg atgattactt
     4921 tcccgttaaa gacactgatt ttaatagtgt ttggcatgat atttacagag atattcgagc
     4981 atctgacagc aatagtacaa aaatatattt caacaatatt gaaataccac taaaattaat
     5041 tgcagatcta attaatgagt taggaattaa tgaattcatt gatagcaaaa aagagcttca
     5101 aatgctaagt tacaaccaat taaataaaat aatcaattca aacttccccc aacaagacct
     5161 atgttttcaa acagaaaaac ttttgtttac ttcactcttt caagaccctg catttatatc
     5221 cgcactaaca tctgcattct ggcaatctct tcacatcacg tcttcctctg tggaacacat
     5281 atatgcccaa attatgtccg aaaacataga aaacaggctt aatttcatgc ctgaacaacg
     5341 agttattaac aattgtggac atattataaa aatcaacgca gttgtcccta aaaacgacac
     5401 tgcaatctct gcctctgggg ggagggctta cgaagttagc tcatcaatat taccatcgca
     5461 cataacatgt aatggtgttg gtattaataa aatagaaaca agctatctcg ttcatgctgg
     5521 aacacttcca agcagtgaag ggcttcgcaa tgctatccct ccagaaagtc gtcaagtatc
     5581 atttgccatc atatctcctg atgtttaagc caatattata ttggctgcca gactgtaaaa
     5641 aaacgttctt cagaacagct ctataaacta ttaaaccaaa gctaaaagga tggggaccaa
     5701 tagaatctca gcccatcctt tttatatcat tatgaaccgc cccgg
//
LOCUS       DAJTMC010000211         5681 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.211, whole genome shotgun sequence.
ACCESSION   DAJTMC010000211 DAJTMC010000000
VERSION     DAJTMC010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5681
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.211"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(25..1944)
                     /gene="dhaR"
                     /locus_tag="OO130_003819"
     CDS             complement(25..1944)
                     /gene="dhaR"
                     /locus_tag="OO130_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase operon
                     transcriptional regulator DhaR"
                     /protein_id="HCR5367042.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWSVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGF
                     HDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            2172..3281
                     /gene="dhaK"
                     /locus_tag="OO130_003820"
     CDS             2172..3281
                     /gene="dhaK"
                     /locus_tag="OO130_003820"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HCR5367043.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECAMQVDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRSFSSLDQTVDEMFDTLLGNGSYHRTLRFWDYQQGSWQEEQQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWDKLGESNVTEQNSNC"
     gene            3253..3885
                     /gene="dhaL"
                     /locus_tag="OO130_003821"
     CDS             3253..3885
                     /gene="dhaL"
                     /locus_tag="OO130_003821"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase ADP-binding subunit
                     DhaL"
                     /protein_id="HCR5367044.1"
                     /translation="MSLSRTQIVNWLTRCGDIFSTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            3896..5314
                     /gene="dhaM"
                     /locus_tag="OO130_003822"
     CDS             3896..5314
                     /gene="dhaM"
                     /locus_tag="OO130_003822"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaM"
                     /protein_id="HCR5367045.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPKIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPAYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVAPPTLRPVPPVSGKAFYYQPVLYTVQAKSTL
                     TVEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDYIAAIFSGHHTLLDDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNRQG"
BASE COUNT         1423 a         1455 c         1400 g         1403 t
ORIGIN      
        1 tgtattagaa atcgtttctg tctttcatac ccggcgctta aactgccctg catcaatgcc
       61 atgttgcttc attttccgcc acagcgtagt gcggccgatt ccaagtaaag ccgacatttc
      121 ctgaatgcga ccgcctgtga cctgggctgc gttaataatt gcctcttttt caacttccgc
      181 aaatgacaga ctggtggaaa ggcgggtggc gctgacatca tctgttgcct gctcggtaaa
      241 cagatgttcc ggcaaatcac tgacgcgaat gcgcccatta tcactactca gagcaagatt
      301 ctcgatgacg ctgtaaagtt caaaatcgtt gcctggccat gcacaagaaa ccaggcgagc
      361 gagggcatcg tcatcaattt tcagccgcgt agagaagcgt ttttcaagac tgcgtaattt
      421 gttattcacc agcgccggaa tgctgccacg ccgcatacgc agaggcggga tggtaatttc
      481 aaatgcatgc agcgcgtaat acagctggcg actaaaacga ttttgttcca ccagcattgc
      541 gaggtccgcg gtcgttgttg caatcacttt gacatcaatt ggtattaaac gccgcgcatc
      601 cagtcgcgtg ataacaccct gcttgataac ctgaagcaaa gcagactgta actccaccgc
      661 cagatattca atcttttcaa gaaacagcgt gccgccgtgc gccagttcca gccgactcag
      721 acggccattt tcattgtccg tacgatcgcc accaataaat tcttccgcca gtgccgcatc
      781 accatataac tcacaattga cggcgatata aggacctgca gcacgctcgc tttcattatg
      841 aattgcctga cttagcagtg ccttgcccac gccctcttct ccacaaagca ggacaggaaa
      901 gctactgcgc gccgcctggc gaccaaaatg aatcaagcgg cgggtttgcg gatcgtcctg
      961 tggcatatga gcaaaggtat ggctgacttt tcctaattga ctggtcatca actgccgcat
     1021 ctgttccaca ggatggagca acaaaataaa gctggttccc tgcgtttcta ttatcggttt
     1081 aagggttatc accgcatcaa taaactgatg ctgactttca aaggttgctt ctacgtgttt
     1141 gagcggatgt gcctgtttta ttgcttgttg caatacggcg ggtaacgtta agagttcagt
     1201 gattgcccgt ccctgacttg ccgtcgcgtc aaggcgcaag acccgcgccg cctgggcatt
     1261 gataaattgc aaattaccct gctcgtccca gctaatcacg ccatcatcca tactttctaa
     1321 cagggcatta agttgattta aatgacggtt agtttcagcg agcaaactgt ccgtcagcag
     1381 taaatttccg acctcgcggg cgattgccaa cgtcaacggc aaatcagctg cggtagtttg
     1441 ctcaaccgga cacgccagcg ctattgttcc cgtcaatcgg cccttgctgt caaacaacgg
     1501 cgttgcacaa aaggcccagt tccagagtgc ctgtttgaaa tgttgatcgg ccatcgtttt
     1561 cacggcctga ccagagatag ccgctaacga tagcgcacaa gtaccaataa ttccctcggc
     1621 gcaatacgtg ccgtcatgga atccaagtgc acttagctgc tgcaaggttt gcggatcgcc
     1681 attacggctg agaatgcagg cggtttcatc gaggataaac agcgcacact ctcgcggtgc
     1741 catatattcc caggcatctt ccagcgcagc ctgcccgagc gtcagcatcg ctttcttacg
     1801 ccgataaata gaagcaaatg tcacgccctg ggcctgatgt ggtacgctcc atgtctcccg
     1861 tttcatcagc ttattgcatc gctcccagga ggttgcaatt aagggagata tgccccgacc
     1921 atcgttgtta aaagcgccac tcatatcccg catcccttat gacaaaaggt gttccggctc
     1981 gcaattttaa gtattaatac acgtaaaagt ttcaatatgg aacattcatt aaagcatatg
     2041 tttcactatg aaacataatt tgggctaatt ttcttttccc ttgcctgatg cacaatggat
     2101 tcatcggcag tcagtggtcg ccgtgtcgtt gaacatcatc catgccctac cgtaattgct
     2161 ggagcaaaat aatgaaaaaa ttgatcaatg atgtgcaaga cgtactggac gaacaactgg
     2221 caggactggc gaaagcgcat ccatcgctga cactgcatca ggatccggtg tatgtcaccc
     2281 gagctgatgc ccctgttgca ggaaaagtcg ccctgctgtc gggtggcggc agcggacacg
     2341 agccgatgca ctgtgggtat atcggtcagg ggatgctttc gggggcctgt ccgggcgaaa
     2401 ttttcacctc accgacgccc gataaaatct ttgaatgcgc catgcaagtt gatggcggcg
     2461 aaggtgtact gttgattatc aaaaattaca ccggcgatat tcttaacttt gaaacagcga
     2521 ccgagttact gcacgatagc ggcgtaaaag tgaccactgt ggtcattgat gacgacgttg
     2581 cggtaaaaga cagtctttat actgccgggc gacgcggcgt tgccaacacc gtattaattg
     2641 aaaaactcgt aggcgcagcg gcggagcgtg gcgactcact ggacgcctgt gcggaactgg
     2701 ggcgtaagtt gaataatcaa ggccactcaa taggtatcgc tctcggtgcc tgtaccgttc
     2761 ctgccgcggg caaaccttct tttaccctgg cggataatga gatggagttt ggcgtcggca
     2821 ttcatggtga gccgggtatt gaccgccgct ccttctcttc ccttgatcaa accgtcgatg
     2881 aaatgttcga caccctgctg ggaaatggct cataccatcg cactttacgt ttctgggatt
     2941 atcaacaagg cagttggcag gaagaacaac aaaccaaaca accgctccag tctggcgatc
     3001 gggtgattgc gctggttaac aatcttggcg caactccgct ttctgagctg tacggcgtct
     3061 ataaccgcct gaccacacgt tgccagcaag cgggattgac tatcgaacgt aatttaattg
     3121 gcgcgtactg tacctcactg gatatgaccg gtttctcaat caccttactg aaagttgatg
     3181 acgaaacgct ggcactctgg gacgccccgg tccacacccc ggcccttaac tgggataaat
     3241 taggagaaag caatgtcact gagcagaact caaattgtta actggctcac tcgttgtggc
     3301 gatattttca gcaccgagag cgagtatctt accggactgg atcgcgaaat tggcgacgct
     3361 gaccacgggc taaatatgaa ccgaggcttt agcaaagtgg tggaaaaact ccctgctatc
     3421 gcagataaag atatcggttt cattctcaag aataccggta tgacgctgct ttccagcgtc
     3481 ggtggtgcca gtggtccgct gttcggtacc ttctttatcc gcgccgcaca ggcgacccag
     3541 gcacggcaaa gcctgacact ggaagagctt tatcagatgt tccgcgatgg cgcggacggc
     3601 gtaatcagtc gcgggaaagc cgaacctggc gataaaacca tgtgtgatgt gtgggtgccg
     3661 gtggtggaat cgttacgtca gtccagcgag caaaatctct ctgttccggt ggcgctcgaa
     3721 gctgccagta gcatcgccga atccgctgca caaagtacga ttacgatgca agcccgcaaa
     3781 ggccgcgcca gttatctcgg tgaacgcagt attggtcacc aggatcccgg cgcgacctcg
     3841 gtgatgttta tgatgcaaat gttggcgtta gccgcaaaag agtaaggaat tggtgatggt
     3901 aaacctggtc atagtttcac atagcagccg actgggagaa ggtgtcggtg aattagcccg
     3961 tcagatgtta atgagtgata gttgtaaaat cgccattgcc gcgggaattg acgatccaca
     4021 aaatcctatt ggtaccgatg ccgtcaaagt gatggaggcc atcgaatctg ttgctgatgc
     4081 cgaccatgtg ctggtcatga tggatatggg tagcgcatta ttgagtgctg aaactgcgct
     4141 ggaattgctg gctcccaaga tcgccgcaaa agtacgtttg tgtgctgcgc cgttggtcga
     4201 aggtacactg gcagcaacgg tcagcgcggc ctcgggagcg gatatcgaca aagttatctt
     4261 tgacgctatg catgcgctgg aagccaaacg tgaacaactg ggtttaccgt cctccgacac
     4321 tgaaatctct gacacatgtc ctgcgtacga tgaagaagcc cgttctctgg cggtggtcat
     4381 aaaaaaccgt aacggcctgc atgtacgtcc ggcctcccgg ctggtttata ccttatcgac
     4441 atttaatgcc gatatgttgc tggaaaaaaa cggcaaatgc gtcacaccag agagtattaa
     4501 ccagattgcg ttactacaag ttcgctataa cgatacgctg cgcctgattg cgaaagggcc
     4561 agaagctgaa gaggcactga tcgctttccg tcagctggct gaagataact ttggtgaaac
     4621 ggaggaagtc gctccaccta ctctgcgtcc cgttccgcct gtttcgggta aagcctttta
     4681 ttatcaacca gttttatata cggtacaggc aaaatcaacc ctgaccgtgg aagaagaaca
     4741 agatcgatta cgccaggcta ttgacttcac gttattagat ctgatgacgt taacagcgaa
     4801 agcagaagcc agcgggcttg actatattgc cgcaatcttt tctggtcacc atacactgtt
     4861 agatgatccg gaactgctgg cggcggcaag cgaactcctt cagcatgaac attgcacggc
     4921 agaatatgcc tggcagcaag ttcttaaaga acttagccag caataccagc aactggatga
     4981 tgaatatcta caagctcgct atattgatgt ggacgatctt ctgcatcgca ccctggtcca
     5041 cctgacccaa acgaaagaag aactcccgca gtttaactcg ccaactattc tactggcgga
     5101 gaacatttat ccttccacag tactgcaact ggatccggcg gttgtaaaag gtatctgcct
     5161 tagcgccgga agtccggtat cccacagcgc cctaatcgcc cgtgaactgg ggattggctg
     5221 gatttgccag cagggtgaga aactgtatgc gatacaacca gaagaaacgc taacgctgga
     5281 cgttaaaacg caacgtttca accgtcaggg ttaagccact gttcggatgg catcgttctg
     5341 atgtcatccg gcaatttact cagcactaat ggtttttaac tacctgttat atttttcacc
     5401 aatttatgat gatttttctt cgctaatcac gaacccaacg ccgctggctg gctaataaac
     5461 agacgttatt tgatcactct atttcgccgt tatttatccc tttaattccc ttttctaaaa
     5521 tgcctgacag ttcgcagaat gagatttcga tcatgcagct agtgcgatcc tgaactaagg
     5581 ttttctgata cttgaatacc gttttattcg gtttcgccaa aggagaatga ttgatgaaat
     5641 cccccgcacc ttctcgcccg caaaaaatgg cgttaattcc a
//
LOCUS       DAJTMC010000212         5680 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.212, whole genome shotgun sequence.
ACCESSION   DAJTMC010000212 DAJTMC010000000
VERSION     DAJTMC010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5680
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.212"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            29..1414
                     /locus_tag="OO130_003823"
     CDS             29..1414
                     /locus_tag="OO130_003823"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCR5367046.1"
                     /translation="MEGGWNEGGKGISIADVMTAGAHGVPREVTEGVIDGLNYPNHEA
                     IDFYHRYKTDIQLFAEMGFKCFRTSIAWTRIFPQGDEQEPNEEGLQFYDDLFDECLKQ
                     GMEPVVTLSHFEMPYHLVTKYGGWRNRKLIDFFIRFASTVFTCYKEKVKYWMTFNEIN
                     NQVNFSESLCPFTNSGILYSPEEDINEREQIMYQAVHYELVASALAVQTGKSINPEFN
                     IGCMIAMYPIYPLTCVPNDMMMATKAMHRRYWFTDVHARGYYPQHMLNYFARKGFNLD
                     ITPEDNAILASGCVDFIGFSYYMSFTTQFSPDNPQLDYVEPRDLVSNPYIDTSEWGWQ
                     IDPAGLRYSLNWFWDHFQLPLFIVENGFGAVDQRQADGTVNDHYRIDYFASHIREMKK
                     AVVEDGVDLIGYTPWGCIDLVSAGTGEMKKRYGMIYVDKDNEGKGTLERIRKASFYWY
                     RDLIANNGENI"
     gene            complement(1471..1689)
                     /gene="marB"
                     /locus_tag="OO130_003824"
     CDS             complement(1471..1689)
                     /gene="marB"
                     /locus_tag="OO130_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HCR5367047.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(1721..2104)
                     /gene="marA"
                     /locus_tag="OO130_003825"
     CDS             complement(1721..2104)
                     /gene="marA"
                     /locus_tag="OO130_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HCR5367048.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            complement(2124..2558)
                     /gene="marR"
                     /locus_tag="OO130_003826"
     CDS             complement(2124..2558)
                     /gene="marR"
                     /locus_tag="OO130_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HCR5367049.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            2770..3435
                     /gene="marC"
                     /locus_tag="OO130_003827"
     CDS             2770..3435
                     /gene="marC"
                     /locus_tag="OO130_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HCR5367050.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            complement(3460..4650)
                     /gene="ydeA"
                     /locus_tag="OO130_003828"
     CDS             complement(3460..4650)
                     /gene="ydeA"
                     /locus_tag="OO130_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HCR5367051.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(4800..5680)
                     /gene="yneK"
                     /locus_tag="OO130_003829"
                     /pseudo
     CDS             complement(4800..5680)
                     /gene="yneK"
                     /locus_tag="OO130_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707449.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
BASE COUNT         1533 a         1255 c         1396 g         1496 t
ORIGIN      
        1 atggggcggc gcggtagccg cgcatcagtt ggaaggtggc tggaatgaag gaggaaaagg
       61 catcagtatc gctgatgtga tgactgctgg cgctcacggg gtgccgcgtg aagtgacaga
      121 aggcgttatc gacgggctta attatcccaa tcatgaagca attgattttt atcatcgcta
      181 taaaacagat attcagttat ttgccgagat gggattcaaa tgctttcgaa cttccattgc
      241 ctggacacga atctttccgc aaggtgacga acaggagccg aatgaagagg gtttacaatt
      301 ttatgatgat ctgttcgatg aatgccttaa gcagggaatg gaacctgtgg tgacgctttc
      361 acattttgag atgccttatc atctggtgac aaaatatggt ggctggcgaa accgtaaact
      421 gatcgacttt tttatccgct tcgcatcaac ggtcttcacg tgctataaag aaaaagtaaa
      481 gtactggatg acgtttaacg aaatcaataa tcaggtgaat ttcagcgaaa gcctgtgtcc
      541 atttactaat tccggtatct tgtattcgcc agaggaagat atcaatgagc gcgaacaaat
      601 aatgtaccag gcggtacatt acgagttagt tgccagtgcc ctggcggtac agactggaaa
      661 atcgatcaat cctgaattta acattggctg tatgatcgcc atgtatccca tctatcctct
      721 gacgtgtgta cccaacgata tgatgatggc cacgaaagca atgcatcgtc gttactggtt
      781 tactgatgtt catgctcgtg gatattatcc gcaacatatg ctgaattact ttgccaggaa
      841 aggattcaac ctcgatatca caccagaaga taacgcgatt cttgccagtg gttgtgtcga
      901 ctttatcggc tttagctact acatgtcttt tacgacacaa ttttcgcccg ataacccgca
      961 actggattat gttgaaccac gagatttggt cagcaaccct tatatcgata catccgaatg
     1021 gggatggcaa attgatccgg cagggctacg ttattcactc aactggttct gggatcattt
     1081 ccagttgccg ctgtttattg tcgaaaatgg atttggtgcg gttgaccaga gacaagctga
     1141 cggcacggtg aacgatcact atcgcattga ttactttgct tcccatattc gggaaatgaa
     1201 aaaagccgta gttgaagatg gtgttgactt aattggctac accccgtggg gctgcattga
     1261 cctggtttct gccggaacag gggaaatgaa aaagcgctac ggaatgattt atgtcgataa
     1321 agacaacgaa gggaagggaa cgctggaacg gatacgtaaa gcgtcgtttt actggtatcg
     1381 ggatctcatc gccaacaatg gcgaaaatat ttgagacttg aggaagatat tacggagatg
     1441 tcaggctctg tgatcagagc ctgacaataa ctacatagcg tgttgattat aatagggcac
     1501 gccgagcgca tccgacttat cactgccagt acccatgtga tttaaatcaa acggtggttg
     1561 ttcctgcgat gggggaacaa ccacgacatt ggcacaagaa gtaacaactg gctgcgtggt
     1621 ttgttccgca acgccctgcg cggaaaagag aataagcgca gctgctattg cggatgaaag
     1681 tggtttcatg attgcctcag tgacgttgtc acgttatcaa ctagctattg taatgattta
     1741 atggatgtaa aaagcgcgat tcgccctgca tattggtcat ccggtattta tgtggcggaa
     1801 catcaaagta atttttgaag gttcgggtca gagtttgttg cgactcgaag ccatatcgtt
     1861 ctgccagata gagtatcggc tcgttacttt ccttcagctt ttgcgcgatt tccgtcatct
     1921 tacggctgcg gatgtattgg cctaatgaat gaccggtttc ttttttaaac atccgttgca
     1981 ggtgccattt agagtaacct gaacgctctg acactttctc cagtgacagt ggcgattcca
     2041 ggttgtcctc gatccagtcc aaaatgctat gaatggtaat agcgtcagta ttgcgtctgg
     2101 acatcgtcat acctcttttt tgtttacggc aggactttct taagcaaatg ctcaagtgtt
     2161 gccacttcgt ccgccgtcag gttttttgtt aattcttgat gcaggtcctg gccaactaat
     2221 tgatggcatt gttcgcatat tgccgcgccg ctggtggtaa gttttaccag tacgccgcgc
     2281 ttatcattcg ggttcggcaa cctttcaacc cagcctttac agaccaggcg atccagcata
     2341 cgggtcagtg ctccaaggtc gacagacaac acttttttca gttcaaccgg agtaatacac
     2401 gccgcgcagc ggatagagca gagcacctta aactgtgccg cggtaatatc cagcggagac
     2461 agatactcgt taagcagacg atctttcttc tgattaacca tatggattaa gcgacccaat
     2521 ggaataattt cattgaacag atcgctggta cttttcacat tagttgccct ggcaagtaat
     2581 tagttgcagg gggtaatatt gcccaggcaa gtataagtca aataaatgaa ttgaccgatg
     2641 ccacgttttg ctaaatcggt tcaagttttg gtcaatgcga tgcaaggtta gctaaagagt
     2701 gacactttat ccttgtttac cccttatact tttcgctgat aatccagata cactggataa
     2761 caaccaccaa tgttagattt gtttaaagca attggcctgg ggctggtggt gttactgccg
     2821 ttagctaacc cattaacaac tgtcgcgtta tttcttggcc tggcaggcaa catgaacagt
     2881 gccgaacgta atcgtcagtc gttgatggcc tcggtgtacg tatttgccat catgatggtg
     2941 gcgtattacg ccgggcaact ggtgatggat acatttggca tttcgattcc cggtctgcga
     3001 attgcaggcg gcttaatcgt cgcgtttatc ggttttcgga tgctctttcc gcaacagaaa
     3061 gcgattgatt caccggaggc gaaaagcaag tcggaagagc tggaagatga acccagtgcc
     3121 aatattgcct ttgtgccgct agcaatgcca agtaccgctg gtccgggaac catagcgatg
     3181 attattagct cggcgtccac agtgcgtcag agttcaacct tcgctgattg ggtactgatg
     3241 gttgcgccgc cgctgatctt tttcctggtg gcggttattt tgtggggaag cctacgcagc
     3301 tccggcgcaa ttatgcggct ggtgggcaag ggtggcattg aagcgatctc ccgtctgatg
     3361 ggattcctgc tggtatgtat gggtgtgcag tttattatta acggcatcct ggaaatcatt
     3421 aaaacgtacc attaaaaaag gcccgaatgg gcctttcaac tattgcgtct gttcttcgag
     3481 tgtcactggc cagcggcgaa atataatgat tgaccatatt aacgcggcaa aagcaggcac
     3541 cgcgcccaca taaccaatca tcgacattga ccagtgcaaa ctcacctgat tacctaccaa
     3601 cgcacccgcc ccgattccaa tattaaatat gccggagaat agcgccatcg cgacgtcggt
     3661 agcatctggt gccagcgcca gcactttaac ctgcatacca agcccgatga tcatcatcgc
     3721 gatcccccag aaaatactca gcaccccgag gtgtatttca ctgttcgccg caggtagcag
     3781 cagtgccaga cacaccagca acagcgcaat cgccgtactc accaacgcag acgcatactg
     3841 attacccagt ttaccgaaaa tcacgctgcc aataatgccc gcaccaccga gtaataacag
     3901 taatgccgtg gcaaagttgg cgctgaatcc cgcaatgttt tgcacaaaag gctcgatata
     3961 gctgtatgcc gtgtaatggg cggtgacaac caccacagtt aacaaataaa tgctcatcaa
     4021 tgccgggcgg cggaacaata gcgggaggct tttcaatgaa ccggaatgct cactgggcag
     4081 taagggaagt aacttaatca ggcacaaaag ggtgataagc gccccaatac caatcgcgaa
     4141 gaaggtcatt cgccaaccga aatactgccc cacaatgcgc ccgagaggta aacctaagac
     4201 catcgccagt gctgtaccgg tggcaattaa actcaaggcc tgtgcccgct tcccggccgg
     4261 agccatacgg atcgccagag acgccgtaat cgaccagaaa atcgcatgtg caaaagccac
     4321 accaatgcga ctgatcacca gaacggtaaa gctccacgat aaaaacgaca gtacgtggct
     4381 ggcaataaac accacaaaca ggcagatcag taatttgcgc cgttcaacct gactggtcat
     4441 taacataaaa ggcaatgaca ttagcgctac tacccatgcg taaatggtca acatgatgcc
     4501 gacctgagcg gtttgcatgt gaaaactttg cgcaatgtca gagagcaggc caacagggac
     4561 aaattcggtg gtgttgaaga tgaaggcggc gactgccagc gtaacgaccc gtagccacgc
     4621 cactttgcgg gaaacagtgt ttgttgtcat aacagattat cgggtgagtt gcgaaaaagt
     4681 gatgaaatga tcttaaaacg gttaatgatg tgaatacaat gcatttgtga tctggatcgc
     4741 gttttccgtt tctctgacgc tgattctgtt ccgcgtcaga gagtcggtaa catcacggtt
     4801 taatgacgtt cagggaggtc tggcgttttg gcggttgcgg gtataggtgg tctgacaggg
     4861 gagcgaggcc tgtttttcac caggtcttcg tgagtaactt ttggcggcgt ttcatttttt
     4921 acaaacagtt tgagggtcct gcgcgccata tgcttgataa ttttatcatc acttaatgat
     4981 gctggcatca acacgtcatt cagatcactt tcattgggat tcataaattg gtgatagtgc
     5041 aataacatat tggcgaaaaa taaaaaaaag tcctgaaaat gaggagattc ttgttctgct
     5101 aaaagggata aaaagcagat gcagacttcg ggtagtacat gtttgttaga acctaatgaa
     5161 tccatcaggc tttgcaataa atcctctttt aattcgccgt ctgttatgtc gagaatggcg
     5221 gtgatcaagt taggatcttt attgacgaac catctgtgaa cctcagagtt actccataat
     5281 cgggggagta tgacaaaatt accgcgagag ataaaattca ggacaagcaa atcaaactgg
     5341 cgtaagtaat ctgcagcggt atggtgatcc ggaggcaggg gttcaatatg tggtgtatgc
     5401 gcttttttga tggggacgcc aaggtggatc attggaaatt gttcaataaa atcattcaga
     5461 aacagaaaga gcacacgctc gacgccggtg tgaaatacgc gccccatagt gacaggcttt
     5521 gggttgggag cgcaaaaata ttaatttctc catcatggcg aaaattatct aaataaatag
     5581 ggtttaaatt tattactacc tgaaattcaa tattttcttt gtgtcttcca ttcttgacgg
     5641 gataagtgag tttaaactga accttttcat tgtatagtgt
//
LOCUS       DAJTMC010000213         5591 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.213, whole genome shotgun sequence.
ACCESSION   DAJTMC010000213 DAJTMC010000000
VERSION     DAJTMC010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5591
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.213"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(35..772)
                     /gene="bamD"
                     /locus_tag="OO130_003830"
     CDS             complement(35..772)
                     /gene="bamD"
                     /locus_tag="OO130_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HCR5367052.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            907..1887
                     /gene="rluD"
                     /locus_tag="OO130_003831"
     CDS             907..1887
                     /gene="rluD"
                     /locus_tag="OO130_003831"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HCR5367053.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNLDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPEDMVELIEVMRADFEEHKDEVDWL"
     gene            1884..2615
                     /gene="pgeF"
                     /locus_tag="OO130_003832"
     CDS             1884..2615
                     /gene="pgeF"
                     /locus_tag="OO130_003832"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HCR5367054.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     EHVEENRKQLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2745..5318
                     /gene="clpB"
                     /locus_tag="OO130_003833"
     CDS             2745..5318
                     /gene="clpB"
                     /locus_tag="OO130_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HCR5367055.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
BASE COUNT         1391 a         1337 c         1515 g         1348 t
ORIGIN      
        1 ctcaagggct gccgttttgt gtttcaggtt tctgttatgt attgctgctg tttgcggcga
       61 tgattttcgc tactttttca gcttgcgcat tcatctgcat ctgacggtat gcattttcca
      121 tcagtggcag cgcatcacgc gtagcctggg tatccgggta gtcgcgcaac atgccttcta
      181 cgcggttaac gacggcaacc catgcaccac gttctgtata gtactcggca acggagtatt
      241 catatttcgc cagacgatct ttcaggaata ccagacgttt ggttgcatcg gtagtgtact
      301 gactgttcgg atagccgcgc accagtttgg aaaagtcact aaacgcagct cgtgcatgtt
      361 gaggatcgcg atcgctacgg tcaacgccaa agaacccttg cagcgcactg tcatccagcg
      421 ccatattggt caggccacgc atgtacatga cataatcgat attcggatgg gtcgggttaa
      481 ggcgaataaa acgatcgatg gcagcctgtg ctaacggcaa atcggcgttt ttatagtagg
      541 cgtagatgag atccagctgc acctgctgcg aatacggacc aaacggatag cgattatcta
      601 atgcttccag ttgcgttatt gcctgtctcc agttaccgtc ctgcagcttt tgttgtgcag
      661 tcgcgtaaat ttcatttggt ggattatcag gtacttcttc ctttgacccc gagcaacccg
      721 ccaaaaacag gcttagtgtg gcggctgcca ccagatattt catgcgcgtc atgacgtttt
      781 gactttcctc aaaatgtaat acgggagatt ctctgttcct gctcccggtt aagaccagct
      841 acaatagcac actatattaa acggcaaagc cgtaaaaccc caacgataaa cgaagaagca
      901 gtatatatgg cacaacgagt acagctcact gcaacggtgt ccgaaaacca actcggtcaa
      961 cgcttagatc aggctttggc cgaaatgttc ccggattatt cacgttcgcg aataaaagaa
     1021 tggatcctcg accagcgcgt gctggtaaac ggcaaagttt gtgataagcc gaaagaaaaa
     1081 gtattgggtg gcgagcaggt tgccatcaac gctgagattg aagaagaagc gcgttttgaa
     1141 ccgcaggata tcccgctgga tatcgtctat gaagatgaag atatcatcgt cattaataaa
     1201 ccgcgcgacc tggtggtaca tcctggcgcg ggtaacctgg atggcacggt actgaatgcg
     1261 ttgcttcatt attatccgcc cattgccgat gtaccgcgtg cgggcatcgt ccatcgtctg
     1321 gataaagaca ccactggcct gatggttgtg gcaaaaaccg ttccggctca gacgcgttta
     1381 gtcgaatctt tgcaaaggcg tgaaattact cgtgagtatg aagcggtggc aattggtcat
     1441 atgaccgctg gcggtacagt agatgaacca atcagccgcc acccgaccaa acgtactcac
     1501 atggcggtgc atcccatggg caaaccggcg gtcactcact atcgcatcat ggaacacttc
     1561 cgtgtgcaca cgcgtctgcg gttgcgtctg gaaaccggac gtacgcacca gatccgcgtg
     1621 catatggcgc atattaccca tccgctggtg ggcgatccgg tttatggtgg ccgtccgcgt
     1681 ccgccaaaag gtgcttcgga agcgtttatc tccacgctgc gtaagtttga ccgccaggcg
     1741 cttcatgcaa ctatgctgcg tctttatcac ccgatctccg gcatcgaaat ggaatggcat
     1801 gcgcctattc cagaagatat ggtggagttg attgaggtta tgcgcgccga tttcgaagag
     1861 cataaggatg aagtggactg gttatgagta agctgattgt cccgcagtgg ccgctgccaa
     1921 aaggtgttgc ggcctgtagc tctacccgta tcggcggcgt gagcttgcca ccttacgact
     1981 ctctcaacct tggcgctcat tgtggtgata acccggagca cgtagaggag aatcgcaagc
     2041 aactttttgc tgcgggaaat ttgccttcaa aaccggtctg gcttgagcag gtacacggca
     2101 aagatgtgct taagctcact ggcgaacctt atgcctcaaa acgggcggat gcctcttata
     2161 gcaatacgcc cggcacggtt tgcgcagtga tgactgccga ctgcctccct gtgctgtttt
     2221 gcaatcgagc gggaacggaa gtcgccgccg ttcatgctgg ctggcgtgga ctgtgcgcag
     2281 gcgtgctgga agagacggtt tcctgttttg ctgataaacc tgaaaacatt ctcgcctggt
     2341 tagggccggc aattggtcca cgcgcgttcg aagtgggggc ggaggttcgc gaggcgttta
     2401 tggcagtaga cgctaaagca agtgcagctt tcattcagca tggtgataag tatctggcgg
     2461 atatttatca gcttgcccgg cagcgtctgg cgaacgtggg tgttgagcaa attttcggcg
     2521 gcgaccgttg tacatatacg gaaaatgaga ctttcttctc ttatcgtcgc gacaagacca
     2581 ccggtcgtat ggcaagtttc atttggctga tataacctaa agaatcaaga cgattcggta
     2641 cgcgtgattt tcttttcaca ttaatctggt caataacctt gaataattga gggatgacct
     2701 catttaatct ccagtagcaa ctttgatccg ttatgggagg agttatgcgt ctggatcgtc
     2761 ttactaataa attccagctt gctcttgccg atgcccaatc acttgcactc gggcacgaca
     2821 accaattcat cgaaccactt catttaatga gcgccctgct gaatcaggaa gggggttcgg
     2881 ttagtccttt attaacatcc gctggcataa atgctggcca gttgcgcaca gatatcaatc
     2941 aggcattaaa tcgtttaccg caggttgaag gtactggtgg tgatgtccag ccatcacagg
     3001 atctggtgcg cgttcttaat ctttgcgaca agctggcgca aaaacgaggt gataacttta
     3061 tctcgtcaga actgttcgtt ctggcggcac ttgagtctcg cggcacgctg gccgacatcc
     3121 tgaaagcagc aggggcgacc accgccaaca ttactcaagc gattgaacaa atgcgtggag
     3181 gtgaaagcgt gaacgatcaa ggtgctgaag accaacgtca ggctttgaaa aaatatacca
     3241 tcgaccttac cgaacgagcc gaacagggca aactcgatcc ggtgattggt cgtgatgaag
     3301 aaattcgccg taccattcag gtgctgcaac gtcgtactaa aaataacccg gtactgattg
     3361 gtgaacccgg cgtcggtaaa actgccatcg ttgaaggtct ggcgcagcgt attatcaacg
     3421 gcgaagtgcc ggaaggattg aaaggccgcc gcgtactggc gctggatatg ggcgcgctgg
     3481 tggctggggc gaaatatcgc ggtgagtttg aagaacgttt aaaaggcgtg cttaacgatc
     3541 ttgccaaaca ggaaggcaac gtcatcctgt ttatcgacga attacatacc atggtcggcg
     3601 cgggtaaagc cgatggcgca atggacgccg gaaacatgct gaaaccggcg ctggcgcgtg
     3661 gtgaattgca ctgcgtaggt gccacgacgc ttgacgaata tcgccagtac atcgaaaaag
     3721 atgctgcgct ggaacgtcgt ttccagaaag tgtttgttgc cgagccttct gttgaagata
     3781 ccattgcgat tctgcgtggc ctgaaagaac gttacgaatt gcaccaccat gtgcaaatta
     3841 ctgacccggc aattgttgca gcagcgacgt tgtctcatcg ctacattgct gaccgtcagc
     3901 tgccggataa agccatcgac ctgatcgatg aagcagcatc cagcattcgt atgcagattg
     3961 actcaaaacc agaagaactc gaccgactcg atcgtcgtat catccagctc aaactggaac
     4021 aacaggcgtt aatgaaagag tctgatgaag ccagtaaaaa acgcctggat atgctcaacg
     4081 aagaactgag cgacaaagaa cgtcagtact ccgagttaga agaagagtgg aaagcagaaa
     4141 aggcatcgct ttctggtacg cagaccatta aagcggaact ggaacaggcg aaaatcgcta
     4201 ttgaacaggc tcgccgtgtg ggggacctgg cgcggatgtc tgaactgcaa tacggcaaaa
     4261 tcccggaact ggaaaagcaa ctggaagccg caacgcagct cgaaggcaaa actatgcgtc
     4321 tgttgcgtaa taaagtgacc gacgccgaaa ttgctgaagt gctggcgcgt tggacgggga
     4381 ttccggtttc tcgcatgatg gaaagcgagc gcgaaaaact gctgcgtatg gagcaagaac
     4441 tgcaccatcg cgtcattggt cagaacgaag cggttgatgc ggtatctaac gctattcgcc
     4501 gtagccgtgc ggggttggcg gatccaaatc gcccgattgg ttcattcctg ttcctcggcc
     4561 caacgggtgt ggggaaaact gagctttgta aggcgctggc gaactttatg tttgatagcg
     4621 acgaggcgat ggtccgtatc gatatgtccg agtttatgga gaaacactcg gtgtctcgtc
     4681 tggttggtgc gcctccggga tatgtcggtt atgaagaagg tggctacctg accgaagctg
     4741 tgcgtcgtcg tccgtattcc gtcatcctgc tggatgaagt ggaaaaagcg catccggatg
     4801 tcttcaacat tctgttgcag gtactggatg atggtcgtct gactgacggg caggggagaa
     4861 cggtcgactt ccgtaatacg gttgtcatta tgacctctaa cctcggttcc gatttgattc
     4921 aggaacgctt cggtgaactg gattatgcgc acatgaaaga gctggtgctc ggtgtggtaa
     4981 gccataactt ccgtccggaa ttcattaacc gtattgatga agtggtggtc ttccatccgc
     5041 tgggtgaaca gcacattgcc tcgattgcgc agattcagtt gaaacgtctg tacaaacgtc
     5101 tggaagaacg tggttatgaa atccacattt ctgacgaggc gctgaaactg ctgagcgaga
     5161 acggttacga tccagtctat ggtgcacgtc ctttgaaacg tgctatccag cagcagatcg
     5221 aaaacccgct ggcacagcaa atactgtctg gtgaattggt tccgggtaaa gtgattcgcc
     5281 tggaagttaa tgaagaccgg attgtcgctg tccagtaatg ataaaacgag cccttcgggg
     5341 ctcgtttttg tctataagtt agacggaaaa gactatattt aagatgtttt gcctgaaaaa
     5401 tgagcgaacg ataaagtttt tatatttttc gcttgtcagg ccggaataac tccctataat
     5461 gcgccaccac tgacacggaa caacggcaaa cacgccgccg ggtcagcggg gttctcctga
     5521 gaatctcaac agagaaaagc aaaaaaatgc ttgactctgt agcgggaaag cgcattatgt
     5581 acaccccgcg c
//
LOCUS       DAJTMC010000214         5529 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.214, whole genome shotgun sequence.
ACCESSION   DAJTMC010000214 DAJTMC010000000
VERSION     DAJTMC010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5529
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.214"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            1..1134
                     /locus_tag="OO130_003834"
                     /pseudo
     CDS             1..1134
                     /locus_tag="OO130_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
     gene            complement(1174..2388)
                     /gene="ydcO"
                     /locus_tag="OO130_003835"
     CDS             complement(1174..2388)
                     /gene="ydcO"
                     /locus_tag="OO130_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HCR5367056.1"
                     /translation="MLRCLYAITYEVTMRLFSIPPPTLLAGFLAVLIGYASSAAIIWQ
                     AAIVARATTAQISGWMTALGLAMGVSTLTLTLWYRVPVLTAWSTPGAALLVTGLQGLT
                     LNEAIGVFIVTNALIVLCGITGLFARLMRIIPHSLAAAMLAEILLRFGLQAFASLDGQ
                     FTLCGSMLLVWLATKAVAPRYAVIAAMIIGIVIVIAQGDVVTTDVVFKPVLPTYITPD
                     FSFAHSLSVALPLFLVTVASQNAPGIAAMKAAGYSAPVSPLIVFTGLLALVFSPFGVY
                     SVGIAAITAAICQSPEAHPDKDQRWLAAAVAGIFYLLAGLFGSAITEMMAALPVSWIQ
                     MLAGLALLSTIGGSLYQALHNERERDAAVVAFLVTASGLTLVGIGSAFWGLIAGGVCY
                     VVLNLIADRNQY"
     gene            2441..2977
                     /gene="sutR"
                     /locus_tag="OO130_003836"
     CDS             2441..2977
                     /gene="sutR"
                     /locus_tag="OO130_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HCR5367057.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSATPSVYDPQQQAMVITSLFPYDPQLCFEHF
                     SIQMASGAISESTPHEKGVIEHVVVIDGQLDLCVDGEWQTLNCGEGVRFAADVTHIYR
                     NGGEQTVHFHSLIHYPRS"
     gene            3050..5011
                     /gene="rlhA"
                     /locus_tag="OO130_003837"
     CDS             3050..5011
                     /gene="rlhA"
                     /locus_tag="OO130_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HCR5367058.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLDQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMNYVKNITAHYRQMLDAIIEERGDLARAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVLKVAKDHLDV
                     AVTEPLANGDGLNVMIKREVIGFRANTVEKTGENQYRVWPNEMPADLHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKLGQTLYYARDVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVAEPAPVYPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDELLKTLPKRKG"
     gene            complement(5103..5333)
                     /locus_tag="OO130_003838"
     CDS             complement(5103..5333)
                     /locus_tag="OO130_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncJ family protein"
                     /protein_id="HCR5367059.1"
                     /translation="MFTKALSVVLLTCALFSGQLMAGHKGHEFVWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGQR"
BASE COUNT         1450 a         1413 c         1399 g         1267 t
ORIGIN      
        1 cgcaggcgca tgtcgtttcg ttttcaatcg tgctctggca cgtcagaatg agaatcatga
       61 ggccggtaat aaatacatcc cttacgggaa aatggcttcc tggctggttg agtggaaaaa
      121 tgccactgaa acgcaatggc ttaaagattc tccctcacag ccattgcaac agtcactgaa
      181 agaccttgag cgggcttaca aaaacttctt ccggaagcgg gctgcttttc cccgattcaa
      241 aaagcgggga cagaatgatg cattccgcta cccgcaggtt aagctcgatc aggaaaacag
      301 ccgtattttt ctgccgaaac tgggctggat gcgctaccgg aatagccgtc aggtcacggg
      361 tgttgtgaaa aatgtcactg tcagccagtc ctgcggtaag tggtacatca gtattcagac
      421 agaaagtgaa gtatccactc cggctcaccc ttcagcatca atggtcgggc tggatgctgg
      481 cgtggctaaa ctcgccacgc tgtcagatgg cacagtcttt gagcctgtaa acagttttca
      541 gaaaaaccag aagacgctgg cgagacttca gcgacagtta agccgcaagg tcaaattcag
      601 caacaactgg cagaagcaga aacgcaaaat acagcgactg cattcctgta tcgcaaatat
      661 ccgcagggac taccttcaca aagtcacaac ggccgtcagc aaaaaccacg caatgatagt
      721 cattgaggat ttgaaggtca gcaacatgtc aaagtcagca ggggtacggt cagccagccg
      781 gggcgcaatg tccgggcaaa atcaggttta aaccgttcga tactggatca gggctggtat
      841 gaaatgcacc gccagcttga gtacaagcag ctctggcgtg gcggtcaggt gcttgctgtt
      901 ccgccagcgt atacaagcca gcgttgcgcg tactgtggtc atacagcgaa agagaaccgc
      961 ctgtcacaaa gtaaattcag atgccaggta tgtggatata cagcgaacgc cgatgtaaat
     1021 ggcgctcgta acattttagc ggcggggcac gccgttcttg cctgtggaga gatggtgcag
     1081 tcaggccgcc cgttgaagca ggaacccacc gaaatgattc aggcgacagc ctgaacgtag
     1141 cagggatcca cgtctttcag ggcgtgtagg atgtcaatat tggtttctgt cagcgattaa
     1201 attcaacacc acgtaacaaa cgcctccggc aattaatccc cagaacgcag aaccaatccc
     1261 gaccagcgtc aatccacttg ccgttaccag aaatgccacc accgccgcgt cgcgctcacg
     1321 ctcattatgc agcgcctgat acaaactgcc gccaatggta ctcaacagcg ccagacctgc
     1381 cagcatctgg atccaactta cgggcagggc agccatcatc tcggtaatgg cactaccaaa
     1441 cagacctgcg agcaaataga aaatgcctgc aacggcagct gccagccaac gttgatcttt
     1501 atccggatgc gcttccgggc tttggcaaat agccgcggtg attgccgcaa taccgacgga
     1561 ataaacgccg aaaggggaaa aaaccagtgc cagcaatcca gtaaatacaa ttaatggcga
     1621 aacaggagcc gaatatccag ctgctttcat tgctgcgata cccggtgcgt tttgcgatgc
     1681 caccgtcacc agaaaaaggg ggagtgcaac gctcaggctg tgagcaaacg aaaaatcagg
     1741 ggtaatataa gtggggagaa cgggtttaaa gacaacatca gttgtgacaa cgtcaccttg
     1801 cgcgatgacg atcacgatcc caataatcat cgcggcaatt accgcatagc gcggcgcaac
     1861 ggccttggtt gccagccata ccagcaacat acttccacac aacgtaaatt gaccgtccag
     1921 actggcaaac gcctgtaaac caaagcgtaa taaaatctcg gcaagcattg ccgccgcaag
     1981 cgagtgcgga ataatgcgca tcagacgagc aaagagtccc gttatgccgc agaggactat
     2041 taacgcgttg gtgacaataa aaacgccgat ggcttcgtta agtgttagtc cctgcaatcc
     2101 ggtgaccaac aaagccgcgc caggcgttga ccatgcggtg agaacaggta cgcgatacca
     2161 taatgtcaga gtcagcgtac tgacgcccat tgccagcccc agcgccgtca tccagccaga
     2221 gatttgtgca gtggtggctc tggcgacaat cgctgcttgc cagattattg ccgctgaact
     2281 ggcgtagcca attaataccg ccagaaaccc cgccagtagc gtgggtggag gaatagaaaa
     2341 cagacgcata gtcacctcgt acgttatagc gtacaggcac cgtagcattt gtccgttata
     2401 acgcacaagt gataaacttc cgttttgccg gaggagtcgc atggaaaatc tcgctcgctt
     2461 tttatccacc acacttaaac aactacgcca gcagcgcggc tggagtcttt cgcgactggc
     2521 agaagcgacg ggcgtttcta aagcaatgtt agggcagatt gagcgtaatg agtccagtcc
     2581 gacggtcgcg acattatgga aaattgccac cgggttgaac gtaccatttt caacatttat
     2641 ttctccgccg cagtccgcta cgccttcagt ttatgatcca caacagcagg cgatggtgat
     2701 cacttcgctg tttccttatg atccacaact ctgctttgaa cacttctcga ttcagatggc
     2761 atctggtgca ataagcgaat cgacgcccca tgagaagggg gtaatcgaac atgtcgtcgt
     2821 cattgatgga caacttgatc tgtgcgttga tggggaatgg cagaccctta attgtgggga
     2881 aggcgttcga tttgctgcag acgtcacgca tatctatcgt aatggtgggg agcaaaccgt
     2941 acattttcat tccctcatcc attacccgcg cagttaagcg ggaaaactat ttcgcaacgt
     3001 cgcgcttgtg gctaaaatag ccgccatttt tcagctactg gataagaatg tgaccgtatc
     3061 ttctcatcga cttgaactgt taagcccggc acgcgatgcc gccattgccc gcgaagctat
     3121 tttgcacggt gccgatgctg tttatatcgg cggccctggt tttggtgccc gtcataatgc
     3181 cagtaatagc ctgaaagata ttgccgagct ggtgccgttt gcccatcgtt atggtgcaaa
     3241 aattttcgtc acgcttaaca ccattttgca tgatgatgag ctggaacccg cgcaacggct
     3301 gattactgac ctctaccaga ccggtgtcga tgcgctgatt gttcaggata tggggattct
     3361 ggaacttgat attccgccga ttgaactgca cgccagtacg cagtgcgaca ttcgtacagt
     3421 tgaaaaagcg aagttcctct ctgatgttgg cttcacgcag attgtgctgg cgcgagagct
     3481 gaatcttgat cagatccgcg cgattcacca ggctacggac gcgaccattg aattctttat
     3541 tcatggggca ctgtgcgtgg cctattcggg tcagtgctac atttctcatg cgcaaacagg
     3601 gcgtagcgcc aaccgtggcg attgctcgca ggcgtgccgt ttgccataca cattgaaaga
     3661 cgatcagggg cgggtggttt cctatgaaaa acatctgctg tcgatgaaag ataacgatca
     3721 gactgccaac ctcggcgcgc tgattgatgc tggtgtacgc tccttcaaga ttgaagggcg
     3781 ttacaaagat atgaactacg tgaagaatat caccgcccat tatcgccaga tgcttgatgc
     3841 cattattgaa gaacgtggcg atctggcgcg cgcttcatca ggtcgtactg aacatttctt
     3901 tgttccatcg acggaaaaga ctttccaccg tggtagcaca gattattttg tgaatgcccg
     3961 taaaggcgat attggcgcgt tcgattcgcc gaaatttatc ggcctgccgg tgggtgaagt
     4021 attgaaagtg gcgaaagatc atctcgatgt tgccgtcacc gagccactgg caaatggtga
     4081 tggcctgaac gtgatgatta aacgtgaagt catcggtttt cgtgccaata cggtcgagaa
     4141 aaccggagaa aatcagtacc gcgtctggcc caatgaaatg ccagcagatt tgcacaaaat
     4201 tcgtccacat cacccactaa accgtaatct tgatcataac tggcagcagg cactgacaaa
     4261 aacctccagc gaacgtcggg tggcggtaga cattgaactg ggcggctggc aggaacaact
     4321 gattctgacc ctcaccagtg aagagggtgt cagcatcacg catacgctgg acgggcagtt
     4381 cgacgaagcc aataacgccg aaaaagcaat gaacaatctg aaggatggtc tggcaaaact
     4441 ggggcaaacc ctctattacg cccgcgatgt gcaaattaat ttgccggggg cgctgtttgt
     4501 accaaacagt ctgttaaacc agttccgccg tgaagctgct gacatgctgg atgctgcgcg
     4561 tcttgccagt taccagcgcg gcagccgtaa accggttgct gagcctgcgc cggtttatcc
     4621 gcaaacgcat ctgagtttcc tcgcgaacgt atacaaccag aaagcgcgtg aattttatca
     4681 tcgctatggt gtgcagctga ttgacgcggc gtatgaggca catgaagaga agggcgaagt
     4741 cccggtgatg atcaccaagc attgtctgcg ctttgccttt aatctgtgcc cgaaacaggc
     4801 gaaaggcaat atcaaaagct ggaaggcgac gccaatgcaa ctggttaacg gcgatgaagt
     4861 attaacgcta aagtttgatt gccgcccatg cgagatgcac gtcattggca aaatcaaaaa
     4921 tcacatactg aaaatgccgt taccgggaag cgtagtggca tccgtaagtc cggatgagct
     4981 gctgaaaaca ttgcctaagc gaaaagggta aaacgccagt tttctggtta ctcacaactc
     5041 attgaatctg catgatattg cctgccgggt aaggcgtcac gccgcatccg gcatcaaacg
     5101 actcaacgtt gtcccgcttc tggtttgcgc gatttttgcc agtaaaaatg ctcgcgcaaa
     5161 ccttccgccg actcttccgc cacagcacgc aattcatcgc tgtccgcttc atgacgcagc
     5221 tgatgatcca cattctttac ccacacaaat tcatgtcctt tgtgccctgc catgagttgt
     5281 cctgagaaca gcgcacacgt taataagaca accgataacg ccttcgtaaa catcctgcca
     5341 cctttttctt accttttgcc gctatgatgc cgatcgtttc ttgaggttat tattcagttt
     5401 tgcaaattag cgcaaagaaa ttctggaatc ttccttcctg attttgcatt gcattctgtc
     5461 gttgcggcga tttagtgcta ttttcgagca aattacacac agaggtaaac actaatgttt
     5521 actttgttg
//
LOCUS       DAJTMC010000215         5505 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.215, whole genome shotgun sequence.
ACCESSION   DAJTMC010000215 DAJTMC010000000
VERSION     DAJTMC010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5505)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5505)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5505
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.215"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            <1..1075
                     /locus_tag="OO130_003839"
     CDS             <1..1075
                     /locus_tag="OO130_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HCR5367060.1"
                     /translation="VEERQRGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETA
                     RLRYGYSSMTTPDTLFELDMDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPV
                     SLVYHRKHFRKGHNPLLVYGYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELG
                     QQWYEDGKFLKKKNTFNDYLDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPE
                     LFHGVIAQVPFVDVVTTMLDESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQA
                     YPHLLVTTGLHDSQVQYWEPAKWVAKLRELKTDNHLLLLCTDMDSGHGGKSGRFKSYE
                     GVAMEYAFLVALAQGTLPATPAD"
     gene            complement(1072..1734)
                     /gene="exoX"
                     /locus_tag="OO130_003840"
     CDS             complement(1072..1734)
                     /gene="exoX"
                     /locus_tag="OO130_003840"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HCR5367061.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWSFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(1758..2414)
                     /locus_tag="OO130_003841"
     CDS             complement(1758..2414)
                     /locus_tag="OO130_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HCR5367062.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLACDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPQGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEYGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            complement(2516..2746)
                     /gene="holE"
                     /locus_tag="OO130_003842"
     CDS             complement(2516..2746)
                     /gene="holE"
                     /locus_tag="OO130_003842"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HCR5367063.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVKLSRLPYEPKLK"
     gene            2885..3259
                     /gene="yobA"
                     /locus_tag="OO130_003843"
     CDS             2885..3259
                     /gene="yobA"
                     /locus_tag="OO130_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HCR5367064.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            3263..4135
                     /gene="copD"
                     /locus_tag="OO130_003844"
                     /pseudo
     CDS             3263..4135
                     /gene="copD"
                     /locus_tag="OO130_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416354.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
     gene            4148..4489
                     /locus_tag="OO130_003845"
     CDS             4148..4489
                     /locus_tag="OO130_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HCR5367065.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(4871..5398)
                     /locus_tag="OO130_003846"
                     /pseudo
     CDS             complement(4871..5398)
                     /locus_tag="OO130_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT         1377 a         1304 c         1371 g         1453 t
ORIGIN      
        1 ggttgaagag cgtcagcgcg ggttaaccag tttgcgccaa attaaccgca agacccggga
       61 agtcattggt attgcctttg atgatccagc ctatgtgacc tggattgcct acaatccaga
      121 acctgaaacc gcgcgattgc gttatggtta ttcttccatg accacaccag acactttgtt
      181 tgaactggat atggataccg gtgagcgtcg tgtattaaaa caaacggaag ttcctggttt
      241 tgatgcggcg aattaccgca gtgaacacct gtggatagtc gcccgtgatg gcgtcgaagt
      301 tccggtttcg ttggtctacc atcgcaaaca ttttcgcaaa ggacacaacc cgttgctggt
      361 gtatggctat ggttcttacg gcgcaagtat tgatgccgat ttcagtttta gccgcttgag
      421 tttgttagat cgtggctttg tctacgccat tgtccatgtt cgcggcggtg gtgagctggg
      481 gcaacaatgg tacgaagacg gaaaatttct gaagaagaaa aatacgttta atgattatct
      541 tgatgcctgc gatgcattgt taaaactggg ctatggctct ccttcgcttt gttatgcgat
      601 gggcgggagt gcggggggca tgttgatggg cgttgcaatt aatcaacgcc cggaattatt
      661 ccacggcgtt atcgcccagg taccgtttgt tgatgttgta acaacgatgc ttgatgaatc
      721 aattcctctt accactggtg agtttgaaga gtggggtaac ccgcaggatc cgcaatatta
      781 cgagtacatg aaaagctaca gcccgtatga caacgtcacc gcacaggctt atccgcattt
      841 actggtaacg accggtttac acgattctca ggtgcaatat tgggaaccgg caaaatgggt
      901 cgctaaattg cgcgagctga aaaccgataa ccatctttta ttgctctgta ccgacatgga
      961 ctcaggccat ggcggtaaat ctggccgctt taaatcgtac gaaggcgtag cgatggaata
     1021 tgcttttctg gtcgcgctgg cgcagggaac attacccgct acgcctgcgg attaagtatt
     1081 ttccagataa tgtttcagtg ttaaacgcag ctccgggctc atgctgtcca ggttattaaa
     1141 tgaccagcgc agatagcccg gatcgcgttc ggcaacgtcg gaaaccgctt tgccacggta
     1201 tttgccaaag gtgaaggtcg tcatcaacga cggacgtccg gtgatatcgg ccatctgttc
     1261 tgccgtccag ccggaggtgt tcataatatc gataagcaac gcggcggtga tataacagtc
     1321 atacaacgcg cggtgatgat gcaggcccgg cggcgtctgt acattgagct tccgtgtttt
     1381 atataacgcc atattgctgt acttgatccc tggccacaaa cgacgggcca gtttcatagt
     1441 gcaaatccac tcaccgggca tctcaggcag tacgcggcgg tcaaagctgg cgttgtgcgc
     1501 gacataccat tcactaccgt aatagtgtgg gatcacatct tcaatccacg gtttatcggc
     1561 gaccatggct tcggtgatgc gatgaatcgc catcgcttgt ggactaatag gacgatcggg
     1621 gcgcaccagg tggctcatgg ggttgacgat ttttccgtca atgacatcaa cagaggcaat
     1681 ctcaacgatc cctccctgca aaccgcaggt ttctgtatcg ataatgcgca acatggcatg
     1741 ctccaggccg aaaaagccta gcgtaatgga atgatatcgc cttgccaacc ctgtgatgaa
     1801 cgctgaccga ctaacaataa tgaaccacga tcggcgcgaa cgatgagacg accatattca
     1861 tcccatagtg cactgttacc acgggcattt gccatcagca cggcaatcga gtactgatgt
     1921 gaaaaaaatt gtaagcgacg ggccgacgcc agcaggtcgg gttctcccag acattgccct
     1981 gtagtaaaca gtgaacaggt cgggtccata tccatacctt gcggttgttc atcgaccacc
     2041 gtgatggttc tggaacgacg gcccagacat gcaccatgac tctgatggta gatcccaggc
     2101 gtttttcgcc acggtgcgaa caccgcaatt ccacgaataa agcgatcgtt atattcaacg
     2161 ggaaggccgg caatgatggt cattcgccag gtcgttgcgg cataacaaag cgggtctaac
     2221 agtgagagat cgggtggggc aggaagggca cgtcgcgaat aatcacacgc cagtaaagaa
     2281 agcgatggaa aaactaacag ctgacattgt tgcctggcgg cggccctcac aaactccaga
     2341 tgatgagcta cctgttcggt gagtgaggtt ttacgaggct catattgcgc tgctgcaact
     2401 ttccagaacg acataatgac ttccttttca aaatgactgc aaattatggg tgcggtttag
     2461 aaaatactca taatttagca ggcgttatgt aagaaagtgt aactctgtat aagttttatt
     2521 taagtttggg ctcgtaaggt aaacgtgaca gtttgaccga agccaaacgg tgggcaataa
     2581 gcctctcgcg aaaccagctg cgcaaatgtt caggctgttc acgttcaacc gcttcagcga
     2641 tcaccggcat attgtagcgt tctttaaatg ccaccccggc cgccgccaaa tcgacattca
     2701 ctttatccat ttctgtttga tccagtttag ccagattctt cagcattctt aatctcctca
     2761 tcattcgcgg gcaaaagtta ctaagagtga gccaggatgg caagccggaa ttgatgatcc
     2821 tcgactcaga cattagacaa ggttatgctc tgtaaaaaag ttattacaaa aagtaataga
     2881 taatatggct tcaactgcac gctcccttcg ctacgcgctg gcaatcctga ccacttcgtt
     2941 ggtgacacct tccgtctggg cacatgcgca tttaacgcat cagtatcctg cggcaaacgc
     3001 gcaagtgaca gctgcaccgc aggcaatcac tttaaacttc tcggaaggtg ttgaaacagg
     3061 attcagtggt gcaaaaatca cggggccaaa aaacgaaaat attaaaacat taccggcgaa
     3121 gcgaaatgag caggaccaaa agcaactgat tgttccactg gccgattcgc tgaaacccgg
     3181 aacctacacc gttgactggc atgttgtttc ggtggacggg cataaaacga aaggacacta
     3241 cacctttagc gtgaaataaa tcatgctggc gtttacctag atcgcgctgc gatttatcca
     3301 tttcacctcg ctgatgctgg tttttggctt cgcaatgtac ggtgcctggc tggccccctt
     3361 gacgattcgt cgcttgctgg cgaagcgttt tctgcgttta caacagcacg ctgccgtctg
     3421 gagtttgatc agtgccactg caatgctcgc cgttcagggc gggttgatgg gaacgggatg
     3481 gacagatgta ttttcgccaa acatttggca ggctgttttg caaacgcagt ttggtggcgt
     3541 ctggttatgg caaattgttc tcgccctcgt aacgttgatc gtcgccctta tgcaaccgcg
     3601 gaatatgcca cgtttgctgt ttatgctcac taccgcgcaa tttattctgc tggcgggagt
     3661 ggggcatgcg acgctgaatg aaggggtaac ggcgaaaatc catcagacta atcacacgat
     3721 tcacctgatt tgtgcggcgg cgtggtttgg gggattactg ccggtgctct ggtgtatgca
     3781 actcatcaaa ggccgttggc gacaccaggc tattcaggcg ctgatgcgtt tttcatggtg
     3841 cgggcatttt gcggtgatcg gcgtactggc aagcggcgtg cttagtgcat tgttgattac
     3901 cggatttccc cccacgctta cgacttactg gggccaactc ttattgctca aagccatcct
     3961 ggtgatgatt atggtggtca ttgcgctggc taatcgttat gttcttgtac cgcgcatgag
     4021 gcaggatgaa gatcgtgcgg caccctggtt tgtgtggatg actaaactcg aatgggcgat
     4081 aggtgcggta gtgctggtga tcatcagcct gcttgcgact ctcgaacctt tttgatggat
     4141 tacgaaaatg atgaaaaaaa gtattctggc gtttctgtta ctcaccagtt ctgcagcggc
     4201 gctggctgca ccgcaggtga ttaccgtcag tcgttttgaa gtgggtaaag acaaatgggc
     4261 gtttaatcgc gaagaggtga tgctgacttg ccgaccgggt aatgctttgt atgttattaa
     4321 tccgagtacc ctcgtgcagt atcctttaaa cgatatcgca caaaaggaag ttgccagtgg
     4381 gaagaccaat gcccaaccca tttcggtgat tcagattgat gatcctaaca atcccggcga
     4441 aaaaatgagt ctggcaccgt ttatagaacg agctgaaaaa ctctgttaat tacctaaaat
     4501 agccttttga tttccaataa aaaaaccgcc tcagttcttt caccagaacg ggcggtttta
     4561 acatttcagc tgatgaccac cacgcttttt attgaccatt ttgcacgcaa actggaaaac
     4621 ctggcgtcgt catctattct taaagggcaa ggcaactaag cctgcattaa tgccaacttt
     4681 tagcgcacgg ctctctccca agagccattt ccctggaccg aatacaggaa tcgtattcgg
     4741 tcttttttta attgcatttg aaatcaatta gttgcaaatg tctccccgaa atttactcga
     4801 atttctgtat tccggtcttt tttggttata tcacaaccaa aatacattta acaatccatt
     4861 tacgttaaaa tcagagcagt aagtacgttt tttctctctc atcaagatac atttttgttg
     4921 tcttctccga tgtgtggcca agtagacgct gagcaaattc ttctccacat gtttctttgt
     4981 acaatcgtcc agccaggctt ctgacttcgt gaaaagttgg tgggttttca ctgaactgga
     5041 tacctgttaa ttttctggct gcgacaaatt tctttgtcag gccgtccggg tgaatgctgc
     5101 cgtcagggct gttttttcta atcccggcac tgattagata atccccccta cttaccatgc
     5161 ggcactgttc aactactgca ccaagtcgta aaccagcgac aggaaggctg agtgacaggg
     5221 ggatagcaat catcattcct gtcttaattt gcctgatgtg gagacgatca tcataaatat
     5281 cactgaaccg catattcgtt atgtcttcgc gacgttgtcc tgttacaagg gctaaatcca
     5341 tagctaatgg gaaccatgcc ggaagttgat ctgctgcctc cctgatgcag ttgtatgtct
     5401 gaggtagcct gagtttaacg gacactcctt cctgaaatag aatggcatca gaaggagcta
     5461 ataatgagca gaaaaaacca acgttactct aaagagttca aagcc
//
LOCUS       DAJTMC010000216         5441 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.216, whole genome shotgun sequence.
ACCESSION   DAJTMC010000216 DAJTMC010000000
VERSION     DAJTMC010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5441
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.216"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(994..1935)
                     /gene="fabY"
                     /locus_tag="OO130_003847"
                     /gene_synonym="yiiD"
     CDS             complement(994..1935)
                     /gene="fabY"
                     /locus_tag="OO130_003847"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HCR5367066.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(1980..2417)
                     /gene="dtd"
                     /locus_tag="OO130_003848"
     CDS             complement(1980..2417)
                     /gene="dtd"
                     /locus_tag="OO130_003848"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HCR5367067.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(2414..3287)
                     /locus_tag="OO130_003849"
                     /pseudo
     CDS             complement(2414..3287)
                     /locus_tag="OO130_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
     gene            complement(3281..3880)
                     /gene="yihX"
                     /locus_tag="OO130_003850"
     CDS             complement(3281..3880)
                     /gene="yihX"
                     /locus_tag="OO130_003850"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HCR5367068.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRVPLATLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(4021..4908)
                     /locus_tag="OO130_003851"
     CDS             complement(4021..4908)
                     /locus_tag="OO130_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HCR5367069.1"
                     /translation="MKTERKNEHYLALQQAFDAPWPGPVGELVTLEKGNIHLQIYPHD
                     GARITSLKAFGSEVLRQWQPQRRAFQYGCFPMVPWAGRLGNATLNAGGQCYSLPANKP
                     PHALHGMACYSTWEIIDKTADSLTLRMPLASPWPWQGEVIQTFLLENDALVLQLEVHS
                     YADTFPASAGWHPWFAKKLTPQNTESLQVLFDADWQEEAGSDELPTGNRISPQAGPWD
                     DCFGFYNGVKVKLLWPGKLAMTMISSANSLVVFDKQPDATCINPLTQAPNAINLTPEL
                     VTPDRPLVIETRWQFTPES"
     gene            complement(4898..>5441)
                     /locus_tag="OO130_003852"
     CDS             complement(4898..>5441)
                     /locus_tag="OO130_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5367070.1"
                     /translation="GIVYISSQLVLSRDIPLTGMLIASAIGIFSCPLVGWLSDRIGQK
                     SLYLSGAGFCVLFAFPFFLLLDSKSTLIIWCSMILGYNLGPTMMFAVQPTLFTRMFGT
                     KVRYTGLSFAYQFSAILGGLSPLIASSLLALGGGKPWYVALFLFAVSVLSFVCVWLIE
                     PTDEQETASYRYIREQSHEN"
BASE COUNT         1473 a         1370 c         1275 g         1323 t
ORIGIN      
        1 tctggggaat atctccccgg cagccttcag ggaaaaatat catcagatgg ctgcttaaaa
       61 aaagaacaaa tggtagtgtc cgctattgcc agtacacctc attctgataa atgaagaaaa
      121 accgtattca tcgccattac caccacctta cctatattac tcattaatgt atgtgcgaaa
      181 attacgccgg aaaatcatta aaaaaaagcg gatttttgca tgttgttttg tttttgcgag
      241 gcgttttcca gaaataataa gagtgttgca gttgtttcat ttattactca aaaatggttt
      301 agttatcgcc caggatgaat tatgggtgct atgaatatat tatattcaga gatatttatt
      361 tgcataatct gaatggtgta gccgaaaaac gtcagcatcc taacagcacc aggatttaag
      421 gtgaaattaa tctttcatca cagaccggtc agtactgcca ccaaccggtc tttacttgct
      481 catcgttata attttgttgg taacagttcg ggaaagttgt ttttctaaat taacaacaga
      541 ataactttct tgtttaatga gattaatatt ggcaaccatt atagcctcct tcccacggtt
      601 taagagcaaa catacaacat aatgattcat aacatacaga gcagtaaatg aatgatactg
      661 acaatgatgc cagcccatta tctttgcata atgtttgtga gctatagatt ttaaaaatat
      721 ttatgaggaa agtatggcac cagaggaggt tttgactatc aaaaatcgct caaacctgaa
      781 ctaagatgat gagttaagca ttatctgcga taattgccac tcagctctac aaataatatt
      841 taaatgctcc tgaacagtga tgtagcaaga cacgtatggt gccaacgaag aataaaaatt
      901 tatagagttc ttattcaata ttaccggcat agacagggaa tgatgtaaat gaccggaatg
      961 aatgagagag gcagcaccgc ccctctccat aagctactct tcttcgttcc cgccctcttc
     1021 atacgggcca aatggcttcg cgggcagaac gatatacgtg ccttcaaaca ctgcacccgg
     1081 cgtctcgtcg ccaaagattt caacttgcat ctgcacccgt gcttttcgtc cgcgcgccag
     1141 acggtcgaga tcgccgctta aggcaccaag gtcggctacc gcatgaggtt taccgctaat
     1201 cggcttgctg tagcggatat gcgcatcggc cagaataatc gttccgccga ggtggcgttc
     1261 gcgcagcatc agccagataa gcccccaacc ggtgagcgtt gccagcgaga ataaactccc
     1321 ggcaaacagc gtatggtgcg gattctgatt accggtttcc ggcatggtgg tgataaattt
     1381 ttgcccggta tattgctgaa tgcgcacgcc cattttttca ctaagcggga tgtgttcgta
     1441 ccacgcctgt tgcagctgcg cgcaccagtc gccgcgatgc aaaatatcat ccagagtggc
     1501 aacaggttta atcatcaaaa aatggcgaat cggcgtggtg gttggtgtgg tgatttctcc
     1561 ctgattaaca aaccccagct tggcgaaaaa ctccaccgcg tcttcacggg cgctacaggt
     1621 cacgcgctta acgccttcct gacgcgccac cgactccagg gtcatcgcca tcaacgtccc
     1681 cagccctttg tcctgcacgt cgggatgaac ggccataaag cgaatggacg cttcattgtc
     1741 ggcattaata tacagtcggc ctaccgccac cagattcccc tgctcgtcga cgaccatctg
     1801 atgatgcgcc atcgcatccc acgcgtcgcg ttccgaacct tttggttgat gcaggggctt
     1861 acgcaacatt tcccagcgaa actgatagta acgctctaac tcttcttctg tttgtggaac
     1921 ccgaaggtga tacatagcga tactctctct tgtttcccgt gacaaccctg gaagctggct
     1981 catacctgca accagaatgt cacggggcca tcattgacca gcgatacctg catatcggca
     2041 gcgaagcgtc ctgtttgcgt gttcatctct tgctggcggc agcgttcgac gaaatagtca
     2101 tataacgcct ctgcgcgatc cggtgatgcg cctttggaga aacttgggcg catcccccgt
     2161 tcggtatctg cggcgagggt aaactgggaa accaccagta cactgccgcc cgcctgttgc
     2221 acgttgagat tcatcttgcc ttcggcatcg ctaaagatgc ggtagccgag cacacgctcg
     2281 cacagacggt ttgctttctg ttcgtcgtca tccttttcga cacccaataa caccaaaagt
     2341 cccgcgccaa tttcgcccgt cacttctccc tccacggtga cgctggcacg ggttacgcgt
     2401 tgaattaatg caatcatggt tcgtcgtctt cttcttgttc agctgcttgt tttagtttgc
     2461 ggtattcccc gagagtgaca gtaatttccg cgccaagcaa gacgatacac catgtccagt
     2521 agacccaaac aaagagaatg gggatcaccg ccagcacacc gtaaatgagc tgatatgacg
     2581 ggaacatggt gatataaagc gcgaaacctt tctttcctgc ttcgaacagg agtgcggcga
     2641 caaacgcgcc gacaatcgcg tcgcggttag gtacgcggat ggtaggaaca atgctgtaca
     2701 gcaaccagaa ggagatccac gacaacagca acggaaaaat acgcagcacg ttgtcgatga
     2761 cagtattgag atcgctcgcc cagcgcaggg agagcaaata ggaactgatc gccagactgg
     2821 cccctgccag cagcggcccc agcgttaaaa tcatccagta cacggcgaac gagtaaattt
     2881 ttggtcgcgc tcgtttactg cgccagatgg tattcaacgc gctatcgatg gagtacatca
     2941 acaataacgc ggtgacgatc agcccgcacg ccccaacggc ggtcatcttg ttggaattgg
     3001 caacaaattg ttcgatatac cgctgaataa catcgccagt agcaggcaga aagttggcaa
     3061 aaataaagtg acgtaactga atgctgacgt cggaaaacat gggaaaagcg gcgaaaagcg
     3121 caaaaacaac ggcaaccagc ggcactaatg agagcaacga ccacataggc aaggttacct
     3181 gccagggttg tcatgttgtc ctcatcaata cgttgccaga gtagttttag ccaggcccat
     3241 agtggacggg tacggtgcct ggctttgtcc tgaatggttt ttagcataac accttcgcga
     3301 aatagtccgg gatggtggtt ttatctttca ccagaatact ggtaatgccc agctgattgg
     3361 ctccttctat attatcggcg ttatcgtcga aaaagaccgt atcgctgggt gaaaaacctt
     3421 ccgcctgcaa aacatgctgg taaattcgtg cttcaggttt gcgcatcccc agatcttgcg
     3481 acagatagat atggtcagca gcatcacgaa tttccgggta ttcttccggc cagaaggtgg
     3541 tatgcaggcg gttggtattg gaaagcacca ccacgcgatg cccctgctca cgcagtttat
     3601 gcatgatggc gatcacttcc gggcgcagcg caacaaacac cgcctgccag ccgtgagaga
     3661 actgctcgta gcttagcggt agagccatct catgacacag cgcctctgcg aacgcttcgt
     3721 cgctaatttc cccacgctca tgctgatgaa acgcctcccc catatgaaaa ctcttcttaa
     3781 gcgtagccag tggaacacgc gttaaatcgc tccaggctcc cagcacacgg ttaaagtcga
     3841 tatcgacaat cacattacct aaatcaaaga tatagagcat ttttgcctcc tttcgcgcca
     3901 caagaaatta actgtagcgg gaaaggatgg gtttggctat gtggcaataa gggtaatctt
     3961 gtgatgctga ttgcgaaaaa gcagagtaat ttcactggct ttttcgcaat agatgaatgg
     4021 ttaagattca ggtgtaaatt gccaacgagt ttcgatgacc agcggcctat ctggtgtcac
     4081 caattccggc gttagattta ttgcgttagg tgcctgagtc agcgggttaa tacaggtggc
     4141 atcgggctgt ttatcaaata ccaccagact gtttgcagaa gagatcatgg tcatcgcgag
     4201 tttaccaggc cacagtaact taaccttcac cccgttataa aaaccaaaac agtcatccca
     4261 gggaccggct tgaggagaga tacgatttcc agttggcagt tcatcactac ccgcttcttc
     4321 ttgccagtcc gcatcaaaaa gcacctgcaa cgattctgta ttttgcggag tcagcttttt
     4381 cgcaaaccac ggatgccagc cagcggaggc cgggaatgta tcggcatatg aatggacttc
     4441 cagttgcaaa accagcgcat cgttctccag caaaaaagtc tgaatcactt ccccttgcca
     4501 gggccacggt gaagccaggg gcatacgtaa agtcagggaa tctgctgttt tgtcgatgat
     4561 ttcccacgtt gagtaacaag ccataccatg caacgcatgt ggtggtttat tggcgggtaa
     4621 agaataacat tgcccaccag cattcagcgt cgcattcccc agtcgccctg cccacggcac
     4681 cattgggaaa cagccatact gaaaagctct gcgctgcggc tgccattggc gtaatacttc
     4741 agaaccaaat gcctttaacg aggttatcct ggcaccatca tgcggataaa tctgcaggtg
     4801 aatattgcct ttttccagcg tcactaactc tcctacaggc ccaggccatg gcgcgtcaaa
     4861 cgcctgttgt agcgccaggt aatgctcgtt ttttcgttca gttttcatga gattgttccc
     4921 tgatgtagcg gtaagaagcg gtttcttgtt catcggtcgg ctcaatcagc cagacgcaaa
     4981 caaaggaaag aactgagaca gcaaataaaa acagagcgac ataccacggt tttcctcccc
     5041 ctagcgccag caaacttgag gcgataaggg ggcttagtcc gccaagaatc gcggaaaatt
     5101 gataagcaaa agaaaggccg gtatatcgta ctttggtacc gaacatgcgg gtgaaaagtg
     5161 ttggctgcac ggcaaacatc atcgtcggac ctaagttgta gccgaggatc atactgcacc
     5221 agatgatgag cgtacttttg ctatccagta ataagaagaa aggaaacgcg aacaggacac
     5281 aaaatcctgc tcccgacaaa tacagacttt tttgacctat acgatctgat aaccatccaa
     5341 ccaatggaca actgaaaata ccgatcgccg aggcaatcaa catacccgtc aggggaatat
     5401 ctcgcgacaa tactaattgg ctggaaatat agacaatacc a
//
LOCUS       DAJTMC010000217         5439 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.217, whole genome shotgun sequence.
ACCESSION   DAJTMC010000217 DAJTMC010000000
VERSION     DAJTMC010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5439
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.217"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            <1..914
                     /gene="adeQ"
                     /locus_tag="OO130_003853"
                     /gene_synonym="yicO"
     CDS             <1..914
                     /gene="adeQ"
                     /locus_tag="OO130_003853"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HCR5367071.1"
                     /translation="PLSLRIGITSGIGLFIALMGLKNTGVIVANKDTLVMIGDLSSHG
                     VLLGILGFFIITVLSSRHFHAAVLVSIVVTSCCGLFFGDVHFSGVYSIPPDISGVIGE
                     VDLSGALTLELAGIIFSFMLINLFDSSGTLIGVTDKAGLIDGNGKFPNMNKALYVDSV
                     SSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTAVVVGVMFLLVMFFSPLVAMVPPYAT
                     AGALIFVGVLMTSSLARVNWDDFTESVPAFITTVMMPFTFSITEGIALGFMSYCIMKV
                     CTGRWRDLNLCVVVVAALFALKIILVD"
     gene            967..1419
                     /locus_tag="OO130_003854"
     CDS             967..1419
                     /locus_tag="OO130_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HCR5367072.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LAAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            1630..2820
                     /gene="nepI"
                     /locus_tag="OO130_003855"
     CDS             1630..2820
                     /gene="nepI"
                     /locus_tag="OO130_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HCR5367073.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRCYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(2861..3154)
                     /locus_tag="OO130_003856"
     CDS             complement(2861..3154)
                     /locus_tag="OO130_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5367074.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            3376..4194
                     /gene="nlpA"
                     /locus_tag="OO130_003857"
     CDS             3376..4194
                     /gene="nlpA"
                     /locus_tag="OO130_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HCR5367075.1"
                     /translation="MKLTTHHLRTGAALLLAGILLAGCDQSSSDAKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVAVGNTFVFPMAGYSKKITTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLHTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVHDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQSPEVAKAAETIFNGGAVPGW"
     gene            complement(4221..5120)
                     /locus_tag="OO130_003858"
     CDS             complement(4221..5120)
                     /locus_tag="OO130_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005030206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR5367076.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLVGTFLLVTHGNPTSLSLSPAALF
                     WGIASAFAAAFYTTYPSTLIARYGTLPVVGWSMLIGGLILLPFYARQGTNFVVNGSLI
                     LAFFYLVVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRPEK"
     gene            complement(5231..>5439)
                     /locus_tag="OO130_003859"
     CDS             complement(5231..>5439)
                     /locus_tag="OO130_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5367077.1"
                     /translation="GMLYFQDLMPEKIGSATTLYANTSRVGWIIAGSVDGIMVEIWSY
                     HALFWLAIGMLGIAMICLLFIKDI"
BASE COUNT         1299 a         1238 c         1393 g         1509 t
ORIGIN      
        1 ttcccttaag tttacgtatt ggtatcacca gcggaattgg attatttatc gccttaatgg
       61 gattaaaaaa tactggcgtt attgtcgcca ataaagacac gctggtgatg attggcgatt
      121 taagttctca cggcgtgttg ttaggtattt tagggttttt tattataacc gtgttgtcat
      181 cacgtcattt tcatgccgcg gtgctggttt ctatagtggt gacgtcttgc tgtggattat
      241 ttttcggtga tgttcatttt agcggcgtct attccattcc gcctgatatt agcggcgtca
      301 ttggtgaagt agatttgagc ggcgcgttaa cacttgaact cgccggtatc attttctcct
      361 ttatgctgat caacctattt gattcatcag gaacattaat tggtgtaact gataaagcgg
      421 gcttaataga tggtaacggt aaattcccca atatgaataa ggcgctgtat gttgatagcg
      481 tcagttcggt ggcgggtgcg tttatcggca cctcgtctgt tactgcctat attgaaagta
      541 cttctggtgt ggcagtcggt ggccgcacgg ggctgactgc ggttgtggtt ggcgttatgt
      601 tcctgttggt tatgttcttc tcaccgctgg tggcgatggt tcctccttac gcaaccgccg
      661 gagcgttaat ctttgttggc gtgctgatga cttcgagcct ggcgcgcgtt aactgggatg
      721 attttaccga atcggtgcct gcgtttatta ccacggtgat gatgcccttt actttctcga
      781 tcaccgaagg gattgcactc ggctttatgt cgtactgcat catgaaagtg tgcaccgggc
      841 gctggcgcga tctgaacctg tgtgtggtgg tggtcgcagc tctgtttgca ctgaagatta
      901 ttctggtgga ttagtaactg gcgagggttt ccagatatca tgagttctga ttacgcagga
      961 gaactcatga tctggataat gctcgccacg ctggcggtag tgtttgtggt tggttttcgg
     1021 gtgctgacat ccggggccag aaaagcgatt cgccgtctca gtgatcggct gaatatcgat
     1081 gtcgtacccg tggagtcgat ggtcgatcaa atgggaaagt cagccggtga cgaattttta
     1141 cgttatttgc atcgcccgga tgagtcgcac ctgcaaaacg ccgcgcaggt gttgctcatc
     1201 tggcaaattg tcattgtcga tggtagcgaa cagaacctgc tgcaatggca tcggatttta
     1261 caaaaagccc gtcttgccgc gccgattacc gatgctcaag tcaggctggc gctgggtttt
     1321 ctgcgcgaaa ccgaacctga aatgcaggat attaatgctt ttcagatgcg ctataacgcg
     1381 ttctttcagc ctgccgaggg cgttcactgg ctgcattaaa accgcttcgt cttatcagcc
     1441 tacagaccgc gcctgacctg tcgggtggat aagacgttca cgccgcatcc ggtaatcttt
     1501 gccctgttcc gtttcgatct taactccagc tcaagcaaca ccgtaaaagt tgcaaaaacg
     1561 ttaaatgcat cacacatttc aatgttaaag tggtcggctt ttcccctgaa acatgccacg
     1621 ggtaacacca tgagtgaatt tattgccgaa aaccgcggcg cggatgccat cacccgaccg
     1681 aactggtcag ccgttttctc ggtggcgttt tgtgtcgcct gtctgattat cgttgagttt
     1741 ttgcccgtca gtttgttgac gccaatggcc caggatttag gcatttcgga aggggttgcc
     1801 gggcaatcgg tgaccgtgac cgcctttgtg gcaatgtttg ccagtttgtt tattacccag
     1861 acaattcagg ctactgaccg ctgctacgtt gttattttgt ttgccgtttt gctgacgctc
     1921 tcctgcttgc tggtttcctt tgctaactca ttcagtttgc ttttaatcgg tcgtgcctgt
     1981 ctggggctgg cgctgggcgg gttctgggcg atgtcggcgt cgctgaccat gcgtctggtg
     2041 ccgccgcgta cggtgccgaa ggcgctgtcg gtgatcttcg gcgcggtttc tattgcgctg
     2101 gtgattgccg cgccgttggg cagtttttta ggcgagctta tcggttggcg caatgtcttt
     2161 aatgcggcgg cggtgatggg cgtgctgtgt attttctgga ttatcaaatc attgccttca
     2221 ctgccaggcg aaccctcgca tcagaaacaa aatactttcc gcttattaca acgtccgggt
     2281 gtgatggcag ggatgatcgc catcttcatg tctttcgccg ggcagtttgc tttcttcacg
     2341 tatattcgcc cggtgtatat gaacctggcg ggattcggcg tggatggctt aacgctggtg
     2401 ctgttgagtt ttggtatcgc cagctttatt ggtacgtcgc tttcgtcgtt cattcttaaa
     2461 cgttcggtaa aactggcctt agcaggcgcg ccgttaatac tggctgtgag tgcgttggta
     2521 ctgacgttgt ggggaagcga taaaatcgtt gctaccggcg tggcgattat ctgggggcta
     2581 acttttgcat tggttcccgt cggctggtca acgtggatca cccgctcgct ggccgatcag
     2641 gcagaaaaag ccgggtctat tcaggtggcg gttattcagc ttgctaatac ctgtggcgcg
     2701 gcaatcggcg gttatgcgct ggataatatt ggtctgactt cgccgttgat gttgtccggc
     2761 acattgatgt tgctgactgc attgttggtt actgcaaagg tgaaaatgaa gaaatcctga
     2821 tatcaggtaa agaagcgttg gtcagtagtt gttgctgttc ttacatatcc gggcattcta
     2881 ccttacccaa cgcttcccaa taattgctct gattattacg ttccatcgcc acgatggcct
     2941 cgcgtatatg ttgtttattg ttttcatctg acaacattaa cttttcccac gcttcatctg
     3001 ataatgacgc ctgtggcgtg caaattttac gtaaatcttg taataccgtg gttttctctt
     3061 ttgcggactg cagtgagcta aaaggcgact cggcgtatac gatgcctggc atagcgaaaa
     3121 aagtgaaaat aagcagtagt gtcgttggtt tcatggattc aaccttttac aaatgtgtat
     3181 cgaatgaagc ataagtgtag tttgctttca tcgctctcat cattctgtac gaaacgcgct
     3241 tgcgtggtca gtaagaagtg ccagacttta tattccactt ttattccttt ttattcttat
     3301 cgatagcgtt tcgtttttta aaccgcagcg accttaccgc tatagtcagg taatcattaa
     3361 taaaaggata aaaaaatgaa actgacaaca catcatctac ggacaggggc cgcattattg
     3421 ctggccggaa ttctgctggc aggttgcgac cagagtagca gcgatgcaaa acacattaaa
     3481 gttggcgtta taaatggcgc agaacaagat gtcgcggaag tcgctaaaaa ggtggcaaaa
     3541 gagaaatatg gcctcgatgt tgaactggta ggatttagcg gttcgttgct accgaacgat
     3601 gcgacaaatc atggcgaact tgatgcgaat gtcttccagc atcgaccctt tcttgaacaa
     3661 gataatcagg cgcatggtta taaactggta gcggtgggaa atacgtttgt cttccctatg
     3721 gcgggttatt ccaaaaaaat cacaaccgtg gcgcaaataa aagaaggcgc gacagtggcg
     3781 atccccaacg acccgaccaa ccttggtcgc gcacttttac tgttgcaaaa agagaagcta
     3841 attactctga aagagggaaa agggttatta cataccgcgc tggatataac cgataatcct
     3901 cgtcatttgc agattatgga actcgagggg gcgcagcttc cgcgcgtact ggatgatccc
     3961 aaagttgatg tagcgattat cagcaccact tacattcagc agacagggct ttctccggtg
     4021 cacgacagcg tatttattga agataagaat tcgccgtatg tgaatatttt ggtggcacgg
     4081 gaagataata agaatgcaga aaacgtgaag gaatttctgc aatcttatca atcacccgaa
     4141 gtcgctaaag cggcagaaac catttttaac ggtggcgcgg tgcctggctg gtaattctca
     4201 ctcatgccgc gccggacgat ttatttttct ggccggcgac gggaatccat tgaaatcaaa
     4261 atcaccgatg acagaatcag cagcgttccc agccagtccg gtaatgtgaa cgtgatcccc
     4321 agcaacagca aagagagtag cgcgctactt aatggttctg cacagctcaa aatgctggct
     4381 tttggaccgc caattaattg tgctcctttc aggtacagac taaatgtcag ggacgtacca
     4441 atgaccacca aataaaaaaa cgccagaatc aaactgccgt taacgacaaa gtttgttcct
     4501 tgtctggcat aaaaaggcaa cagaatcaga ccgccaatca gcatactcca gccgacgact
     4561 ggtaacgtgc catagcgggc aattagcgtt gagggatagg tggtatagaa tgcagcagca
     4621 aatgccgagg caatgcccca gaacaacgcg gcaggagaga gcgataatga cgtcggatta
     4681 ccgtgtgtca ccaataaaaa agtcccgacc agcgatgtca aaatagcgca gaaaaccaga
     4741 atgcccgggc gcgatttacg caccagtgag aaccaggcga cgataatcgt cggtgagagg
     4801 aattgcagca ccgttgccgt ggctgcgttc gatttttcga tggttagcaa aaaagtgagc
     4861 tgtacagtta gcgcgccaac cacggaaaaa atcagcaggc taatggcatc tttatgattg
     4921 ttaataatag aaaagatttt atcgccatga acaaatgaca gcgtcagtag aatcaaaccg
     4981 gcgaatatca aacgcgtcat agtcaaaaac tgcgacgaca tctggctttg ctccatgatg
     5041 tattgcgcgc agacccctga acttccccac aacacggcgg caatcagaac gttcagcatc
     5101 ccctttctgg tggaacccat ttttccctct gcatactgtt ttgtcgttta tgtatgcagg
     5161 catgatagca aaaatggcga ggatggctct gtttgcgcca cgccgatttt tcttagccca
     5221 gactggtgag ctaaatatct ttaataaaca gcaggcaaat catcgcaata cccaacatcc
     5281 ctatcgccag ccagaacaac gcatggtagc tccagatttc aaccataatt ccgtcaacag
     5341 agccggcgat aatccagccg acgcgtgaag tatttgcata taacgttgtc gcagagccta
     5401 ttttttcagg cattaagtcc tgaaaataaa gcatgccga
//
LOCUS       DAJTMC010000218         5318 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.218, whole genome shotgun sequence.
ACCESSION   DAJTMC010000218 DAJTMC010000000
VERSION     DAJTMC010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5318
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.218"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            <1..1279
                     /locus_tag="OO130_003860"
     CDS             <1..1279
                     /locus_tag="OO130_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HCR5367078.1"
                     /translation="EFVWDLFTAWLTAGAPSKESWAFTALGVLGNDDTARKLTPLIRA
                     WPGESQHKRATVGLDILAAIGSDIALMQLNGIAQKLKFKALQERAKEKIADIAESREL
                     TVAELEDRLAPDLGLDDNGSLLLDFGPRQFTVSFDETLKPFVRDASGSRLKDLPKPNK
                     SDDESRSNDAVNRYKLLKKDARIVAAQQVARQESAMCLRRRWSPENFKLFLVEHPLVR
                     HLTRRLIWGVYSAENQLQACFRVAEDNSYSTADDDLFTLPEGGISIGIPHVLEISPTD
                     AAAFGQLFADYELLPPFRQLDRNSYALTGAERNASELTRWAGRKCPSGRVMGLANKGW
                     IKGTPQDGGWIGWMIKPLGRWSLVMEIDEGFAVGMSPAELSAEQLLSKLWLWEGKAES
                     YGWGSNSTQEAQFSVIDAITASELINDIEALFE"
     gene            1289..1531
                     /locus_tag="OO130_003861"
     CDS             1289..1531
                     /locus_tag="OO130_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005069643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HCR5367079.1"
                     /translation="MDKELPWLADNAQLELKYKKSKTPLSHRNWPGEPVSVITESIIQ
                     TLGDELLQKAEKKKRLSGVMKIFHWSGSPPSRRPST"
     gene            3055..4920
                     /locus_tag="OO130_003862"
                     /pseudo
     CDS             3055..4920
                     /locus_tag="OO130_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001543415.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            4982..>5318
                     /locus_tag="OO130_003863"
     CDS             4982..>5318
                     /locus_tag="OO130_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5367080.1"
                     /translation="MIVQKELVAIYDYEVPVPENPFSFRLEIHKCSELFTGSVYRLER
                     FRLRPTFHQRDREDVDPLINDALIYIRDEFIDERKLRGESPEPVPVIFNCELQNIFNQ
                     LIEYYFIKVG"
BASE COUNT         1370 a         1341 c         1365 g         1242 t
ORIGIN      
        1 tgaatttgtc tgggatctgt ttaccgcctg gctgactgcg ggcgcgccgt cgaaagagag
       61 ttgggcgttt actgcgttag gcgttctcgg taacgatgat accgctcgca aactgacgcc
      121 attaatccgc gcctggcctg gtgaatccca gcataaacgc gccaccgttg ggttggatat
      181 tctcgccgct atcggtagtg atattgccct gatgcagctt aacggcatcg cccagaaact
      241 taaattcaaa gcattacagg agcgggcaaa agaaaaaatt gccgacattg ccgagagccg
      301 cgaactcacg gtggcggagc ttgaagatcg gttagcaccg gatctcggtc tggatgataa
      361 cggttcgttg ctgctggatt tcggcccacg ccagttcacc gtcagctttg atgaaacctt
      421 aaaaccgttt gtgcgtgacg cttccggcag ccgcctgaaa gacctgccca aaccaaacaa
      481 aagcgatgat gaatcgcggt cgaacgatgc ggttaaccgc tacaaattgc tgaaaaaaga
      541 tgcgcgtatc gtcgccgccc agcaggtagc aaggcaggaa tccgccatgt gcctgcgtcg
      601 ccgctggtcg ccagaaaact ttaagctctt cctggttgag catccgttgg ttcgccactt
      661 aacccgccgt ctgatttggg gcgtttatag cgccgaaaac cagctacagg cgtgctttag
      721 ggtggcggaa gataacagct acagcaccgc tgacgatgat cttttcaccc tgccggaagg
      781 cggtatctct atcggcattc ctcacgttct ggaaatatca ccgacggatg ctgccgcctt
      841 tggtcagctt tttgccgact acgaactgct accaccattc cgtcagcttg accgtaacag
      901 ttacgccctg acaggagccg agcgcaacgc cagcgaactg acccgctggg caggcagaaa
      961 atgcccgagc ggtcgggtca tggggctggc gaataaaggc tggataaagg gcaccccgca
     1021 ggatggaggc tggatcggct ggatgatcaa acccctgggc cgctggtcgt tagtcatgga
     1081 aatcgatgaa ggttttgcgg taggcatgtc gccagccgaa ctcagcgctg agcagctctt
     1141 aagcaagctg tggctatggg aaggcaaagc agaaagctat ggctggggga gtaattcaac
     1201 acaggaagcg cagttctccg taatcgatgc catcaccgcc agcgagctaa ttaacgatat
     1261 tgaagccctg tttgaataag gaaagagcat ggacaaggaa ttaccgtggc tggcggataa
     1321 cgcccaactg gaactgaaat ataaaaaaag caaaacgccg ctcagtcatc gcaattggcc
     1381 gggcgaaccg gtgtctgtta tcactgaaag catcattcag acactgggtg atgaattgct
     1441 acaaaaagct gagaagaaaa aaagattgtc tggcgttatg aaaatttttc actggagtgg
     1501 cagtccgcca tcacgcaggc catcaacttg atcggcgaac acaaaccctc agtcccggcc
     1561 cggacaatgg cggcgctagc ctgtatcgcg caaaatgaca gccaacagtt gctcgacgaa
     1621 atcgtccaac aagaggggct ggaatatgcg actgaggtgg taattgcacg cctgtttatt
     1681 gcgcggtgtt atgagagtga tcctctggta gtgacattgc agtatcagga cgaggattat
     1741 ggctatggtt atcgctcaga aacctataac gaattcgatc tccgactgcg taagcatctc
     1801 tctctggcag aggaaagctg ctggcagcgt tgcgccgaca aactcattgc cgcactacca
     1861 ggaataacca aagttcgccg cccttttatt gcgctgatcc tcccggaaaa accagaaata
     1921 gccaatgagt tggtaggcct tgaatgcccg cgaactcatt ttcattctaa gaagtggtta
     1981 aaagttgttg ctaatgaccc cacagcggtg agaaaactcg aacactactg gagccaggat
     2041 atatttagcg atcgagaagc cagctacatg tcgcatgaaa accacttcgg ctacgcggcc
     2101 tgcgccgccc ttttgcgcga acaaggactg gcagccattc cgcgcctcgc gatgtatgcc
     2161 cataaagaag attgcggcag tctgctggta caaattaacc atccgcaagt catccgcacc
     2221 ttgctactgg tggctgataa aaacaaaccc agcctgcaac gtgtagctaa ataccataaa
     2281 aacttccccc atgcgacgct cgccgcactg gcagaactgc tggcgttaac agaaccacca
     2341 gcccgccctg gttatccaat catcgaagac aaaaagctgc ctgcacagca aaaagcacgc
     2401 gatgaatact ggcgtacgct gttacaaacg ctgatggcat cgcagccaca actggcagaa
     2461 gaggtgatgc agtggttaag tactcaagcc agggcagtgc tgaatagtta tttatcggca
     2521 ccgcccaaac cggttattga tagtaccgat aacagcaatc tgcctgaaat cctcgtttcg
     2581 ctaccgtggc gtagtaagaa aaaaatgaca gctccacgtc ttgatttggc accgctcgaa
     2641 ttaactccgc aagtttactg gcaaccaggc gaacaagaga ggcttgccgc cactgagtct
     2701 gcccgttatt tcagcacgga atctcttgcg caacgcatgg aacaaaaaag tggacgagtt
     2761 gtattacagg aactgggttt tggggatgat gtatggctgt ttctgaatta tatactcccc
     2821 ggaaaactgg atgctgcacg caattcactc attgttcagt ggcattacta ccaggggcgg
     2881 gttgaagaga tcctgaatgg ctggaactcc ccggaagcac aattagcaga acaggcgctc
     2941 cgcagcggtc acatagaagc gttaattaac atatgggaaa atgacaacta ctcacgttat
     3001 cgtccggaaa agagtgtctg gaacctgtat ttattggcac agttgccgcg tgagatggct
     3061 ttgaccttct ggctgcgtat caatgagaaa aagcatctgt tcgcgggtga ggactatttt
     3121 ctcagtatcc tcggattgga tgcgctacca ggtctgctgt tggctttttc acatcgtcca
     3181 aaagaaacat ttccgttaat tttaaatttt ggcgcaacag aactggcgct gcctgttgcc
     3241 cgcgtctggc gacgtttcgc ggcgcagcgt gatctggctc gccagtggat tttacaatgg
     3301 ccggaacata cggctagtgc acttatccct cttgtcttta ccaaacccag cgataatagc
     3361 gaagccgcat tacttgccct gcgtttactg tacgaacagg gacatggcga attgctacaa
     3421 accgtggcaa accgctggca gcgtacagat gtatggtctg ccctggagca gttgcttaaa
     3481 cagggtccaa tggacattta cccggcacgc attccaaaag cccctgattt ctggcatccg
     3541 gcaatgtggt ccaggcctcg acttatcact aataatcagc ctgttaccgg tgacgctctg
     3601 gaaattatcg gcgaaatgct gcgctttacc taggggggac gtttttatag cgggctggaa
     3661 caactgaaaa cgttctgcca gccacaaacg ctggccgctt ttgcctggga tctcttcact
     3721 gcctggcaac aagcgggggc ccccgcaaaa gacaactggg catttctggc attacgtctc
     3781 tttggtgacg aaagcacggc acgggatctg acgacacaga tcctcgcctg gccacaagaa
     3841 ggcaaatctg cccgtgctgt cagtggtctg aatatactta ccctgatgaa taatgatatg
     3901 gcgctgatgc agctgcatca tgtatcacaa cgggcgaaat cacgcccctt acgtgaaaac
     3961 gcagcggaat ttcttcaggt ggtcgcagaa aatcgcgggc taagccagga agagttagcg
     4021 gacagattag tcccaaccct gggccttgat gatccgcagg cgttgagttt tgattttggt
     4081 ccccggcagt ttaccgttcg cttcgatgaa aacctcaacc cggttatctt tgatcagcaa
     4141 aacgttcgcc agaaaagcgt tccccggttg cgcgccgatg acgatcaact gaaagcgccc
     4201 gaggcactgg cccgactaaa agggctaaaa aaagatgcta ctcaggtgag caaaaacctg
     4261 ctcccgcgtc ttgaagctgc cctacgtacc acccgacgct ggtcgctggc agattttcat
     4321 tctctgtttg ttaatcatcc ctttacccgt ctggttaccc agcgattaat atggggggtt
     4381 tatccggcaa atgaaccgcg ttgtttactc aaagcctttc gtgtggccgc agagggggag
     4441 ttctgcaatg cgcaagatga gccaattgac ctgcctgcgg acgctctgat tggcattgcc
     4501 cacccgttag aaatgacagt agaaatgcgc agtgaatttg cacagctttt tgccgattac
     4561 gaaattatgc cgccttttcg ccaattgtcg cgccgcacgg tgctgctcac acctgacgag
     4621 tcaaccagta acagcctgac tcgctgggaa ggtaaatccg ctaccgttgg gcagcttatg
     4681 ggaatgcgat acaaaggctg ggagtcaggt tatgaggacg catttgtcta tgacctgggt
     4741 gagtaccggc tggtccttaa gttttcaccc ggttttaacc actacaatgt tgatagcaaa
     4801 gcgctaatga gcttccgttc tcttcgagtg taccgtgaca ataaatccgt cacttttgcc
     4861 gaacttgatg tgtttgattt gagtgaggcg ttaagcgcac ctgacgtcat tttccattaa
     4921 aaacacagcg ggcagtgatg caactgcccg ttatcaacac gacttaccag cggggaaagc
     4981 gatgatcgtg caaaaagagc tggttgctat ttacgattat gaggtccctg tacctgaaaa
     5041 tccgttttcc ttcagacttg agatccataa atgctctgaa ttatttacag gttccgtcta
     5101 tcgactggaa cggttccggc tacgtccaac atttcatcaa cgtgatcgag aggatgttga
     5161 cccgctaata aatgatgcgt tgatttatat aagagatgag tttattgatg agcggaaatt
     5221 acgaggtgaa tcacctgaac ctgtaccagt aatttttaat tgtgaactac agaatatatt
     5281 caaccaacta atagaatatt actttattaa agtgggaa
//
LOCUS       DAJTMC010000219         5207 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.219, whole genome shotgun sequence.
ACCESSION   DAJTMC010000219 DAJTMC010000000
VERSION     DAJTMC010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5207
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.219"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            <1..117
                     /locus_tag="OO130_003864"
     CDS             <1..117
                     /locus_tag="OO130_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3829 domain-containing protein"
                     /protein_id="HCR5367081.1"
                     /translation="DWDKEQLQDANSSWMVEDSFPRALREYNEMVDDYNSLR"
     gene            complement(167..1915)
                     /locus_tag="OO130_003865"
     CDS             complement(167..1915)
                     /locus_tag="OO130_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative frv operon regulatory protein"
                     /protein_id="HCR5367082.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARISASGSAGYQLEIFERRSFFQLLQKHDNDYRLLVLLLLNTFTPRAQLASALN
                     LPETWVAERLPRLKQRYERACCLASRPGLGHFIDETEEKRVILLANLLRKDPFLIPLA
                     GVTRDNLQHLSTACDNQHRWPLMQGDYLSSLILAIYALRNQLTDEWPQYPGDEIKQIV
                     EQSGMFLGDNAVRTLTGLIEKQHQQAQVISADHVLGLLQRVPDIASLNIIDTQLVENI
                     TGHLLRCLAAPVWIAEHRQSSMNNLKAAWPAAFDMSLHFITLLREQLDIPLFDSDLIG
                     LYFACALERHQNERQPIILLSDQNAIATINQLAIERDVLNCRVIIARSLSELVAIREE
                     IEPLLIINNSHYLLEDAVNNYITVKNIITAAGIEQIKHFLATAFIRQQPERFFSAPGS
                     FHYSNVRGESWQHITRQICAQLVAQHHITADEAQRIIAREGEGENLIVNRLAIPHCWS
                     EQERRFRGFLITLAQPVEVNNEVINHVLIACAAADARHELKIFSYLASILCQHPAEVI
                     AGLTGYEAFMELLHKG"
     gene            complement(1915..2985)
                     /locus_tag="OO130_003866"
     CDS             complement(1915..2985)
                     /locus_tag="OO130_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCR5367083.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GVKIPGVIGSVAPHALTEKQRQRPLSFDEMFIDIGANSRDEVEKRGVAMGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAALKSCAAH
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RDFLTTLTAEKVNAFSQFRQMD"
     gene            complement(2975..4426)
                     /locus_tag="OO130_003867"
     CDS             complement(2975..4426)
                     /locus_tag="OO130_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HCR5367084.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFTGKKVYEIAISQALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEMQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            complement(4437..4883)
                     /locus_tag="OO130_003868"
     CDS             complement(4437..4883)
                     /locus_tag="OO130_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HCR5367085.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATIALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHKEFIHQLQQGDTDQVLALLNQTLSS"
BASE COUNT         1318 a         1342 c         1316 g         1231 t
ORIGIN      
        1 gactgggata aagagcaact tcaggacgca aactcaagct ggatggtcga agactctttc
       61 ccgagagcat tacgcgagta caacgaaatg gttgatgact ataatagcct gcgttaaacg
      121 tttttgatga taaatgcagt gtcggatgcg gcgcatctga cacagttcac cccttgtgaa
      181 gtaactccat aaatgcctca tatcctgtta acccggcaat aacctctgcc ggatgctgac
      241 acaatatgct tgccagatag ctaaatattt ttagctcatg acgcgcatcg gcggcggcgc
      301 aggcgatcaa gacatggtta atgacttcgt tattcacctc aactggttgg gcgagggtaa
      361 taagaaatcc acgaaaacgt cgctcctgtt cgctccagca atgtgggatg gcgaggcgat
      421 taacaatcag gttttcacct tcgccttcgc gggcgatgat gcgttgtgct tcatcggcgg
      481 taatatggtg ttgtgcgact aattgcgcac aaatttgccg ggtaatatgt tgccagcttt
      541 caccgcgtac attcgaataa tgaaaacttc cgggggcaga gaaaaaacgc tccggttgct
      601 ggcgaataaa tgccgtcgcc agaaaatgtt ttatttgttc gataccggca gccgtaatga
      661 tattttttac ggtgatgtaa ttatttaccg cgtcttccag taaataatgg ctattgttaa
      721 tgatcaataa cggctcaatc tcttcgcgaa tggcaacaag ttcgcttaag ctacgggcaa
      781 taattacccg acaatttaaa acatcgcgct caatggcgag ctgattaata gtggcaatcg
      841 cgttctggtc cgagagcaaa atgatcggct ggcgttcgtt ttgatgccgc tccagcgcac
      901 aggcaaaata caaaccgatc agatcgctgt cgaaaagggg aatatcgagc tgttcacgca
      961 gtagcgtaat aaagtgcaga ctcatatcaa aggccgcagg ccaggctgct ttcaggttat
     1021 tcatgctact ttggcggtgc tcagcaatcc acaccggtgc ggcaaggcaa cgtaataaat
     1081 gcccggtaat attctcaacc aactgcgtat caataatatt caatgacgcg atgtctggca
     1141 ccctttgcag caaccccagc acatgatcgg ctgaaattac ctgcgcttgc tgatgctgtt
     1201 tctctatcaa acctgtcagg gttcttacag cgttatcacc aagaaacatg ccgctttgtt
     1261 caacgatttg ttttatctcg tcaccgggat attgcggcca ctcatcggtc agttgattac
     1321 gcagggcgta aatcgccaga atcaggctgg agagataatc accctgcatg agcggccagc
     1381 ggtgttggtt gtcgcaggcc gtggataaat gctgaaggtt gtctcgtgtt acgcccgcca
     1441 gcggaattaa aaacggatct ttgcgcagca agttcgccag caagataacg cgcttctctt
     1501 ctgtctcatc aatgaaatgg cccaaaccgg ggcggctggc caggcaacaa gcgcgttcat
     1561 aacgctgttt taaccggggc agacgctctg cgacccacgt ttctggcaaa ttaagcgccg
     1621 aggcgagttg cgcacgggga gtaaaggtat tcagtaataa cagtaccagc agccgatagt
     1681 cgttatcgtg cttttgcagc aactgaaaaa agctgcggcg ctcgaagatt tccagctgat
     1741 agcccgcact accactggcg gaaatgcggg ctttgccgtt aagggtaaag ttgagatagt
     1801 caatatcacg caggatggtc ctgcctgaaa cgccagtctg ttgcgccagc tcaccaggcg
     1861 tgcgcggctg ttgctccagc agatcgacaa tctttaactg gcgttcgttt agcattaatc
     1921 catctgacgg aactggctaa acgcgttgac tttctccgcg gttaaggtgg tcagaaaatc
     1981 ccgtatcaac gtgagcagag catcgtaatc ggcttttgaa atcataccgc tgttagcgtg
     2041 cagatagcgg gttggcaggc acagcgcaac aaccggacgc ccgccgccca tcacgttgta
     2101 gcgcccgcca tccgtcgcac cggttttcat ggtggaaaat tgcagcggta aatcgttatg
     2161 tgcggcacag ctttttaacg ctgctaccag tttctggttg gggaagtagc gcttgtcaaa
     2221 cagcatcagc cccggcccct ggcccagttt cagcgggtat ttaatgttat caatgcccgg
     2281 aacatcgcct gcgacagcgg tatccagcac gatcactaca tccggtttaa tgtgttccgc
     2341 cgaggtttgt gccccgcgta gcccaacttc ttcttccacg ctgccgacgc catacagcgt
     2401 aatttcggga ttattcaccg tctgcaatag ttcagccatc attgcgcagc cgatgcggtt
     2461 atccagcgcc ttgccgacca ctttatcttc gccccagcag gcaaaattgg cttccgggct
     2521 aataaaatcg cccatcgcca caccgcgttt ttccacctca tcgcgactgt ttgcgccaat
     2581 atcaatgaac atctcatcaa atgacagcgg ccgttgcctt tgcttttccg ttaacgcatg
     2641 aggcgcgacg gaaccaatca caccagggat tttcactccc ttgtgtgtgc gtatggttac
     2701 ccggtggttg agcatcgact gattccacca gccaccaatg gtggtgaaac gcagaaaacc
     2761 gctctcgtcg atgtgggtga ccataaagcc gacttcatcc atatgcccga caacggcaac
     2821 ttttggacct ttattcccct tacgggcaac aaagctgccc agaccatcaa aggtgatctc
     2881 attaacgcaa ggttccagcg tgtttatcag aatgtcgcga acttcctgtt catcgccgct
     2941 gacggcgctg gcttcgcaca actgttgcag taactcaatg ttcatggaca agctcctgtt
     3001 gcgcggcttt cgctttgcgg cgtaaccaca atcctttcag gacgataatg aatgaaatgt
     3061 tcagcgcgag gccaatcgcc agcaccagat aaaacgaacc caccggcgac atcaggccga
     3121 tcagcgggtc aaagataccc aaaccaggtg ccagacgttt gattccgaaa gcgatcacca
     3181 gcacgccggt aatgccaccc gccagcgtat tggcggtaat catcggcaac ggagctgcca
     3241 gagcgtaagg tatagccggt tcggttgcga ctgtggcacc gaccacaatg gcactactgg
     3301 cagcctcttt ttcctgtcgg gtaaacagtt tgggcgcaat gaacgtcgca agacccgccg
     3361 caacgggcgg catcaacgcg acaacgccga cgatggcgta ccagtcataa atgtgttttt
     3421 ccagcagtga gaagcagaag aaccaggcgg ttttattgat ggggccgccc atatcgaacg
     3481 ccagcatcgc accgaccaga aacgctgctg caaacttcat tgatggagga atggtattca
     3541 ggaagtggag cagcccgccc atgaggtcgg acatcaccgg cccgataacg tagtaagtga
     3601 gcacgccgaa aaccagcaga gtaacgaacg ggatcagcat tgaaccgagc agcggttgta
     3661 atgctttgcc aaggcgcact ttgcggaacc agaagacgaa atagccaatc gccagcccca
     3721 gcaccaccgc gccgaggaat cccgcacccg actgggtgcc gagcagcgct ttgtcgttcg
     3781 ccagatagca aaccagaaac gccggagcga aagccggttt atcggcaatc gacgaggcaa
     3841 tataagcgcc catgatgggg atcataaagg tgaagccaag gtaaccgatg gattcaacta
     3901 cccaggtaaa cgacggcgcg cctttcgaca tatcggtata cggcaaaccg aactgcacca
     3961 gcatgttggc aagcgccacc aggataccac cgccgatgac aaacggcagc gccgcagaga
     4021 cgcccgccat caggtggctc atcacgctgc cgctttgcat ttcctgtttg ccaagcttca
     4081 cgccactatc tgcggcaaaa agctgcgagt ttgtcggtaa ttcgctgaaa atctggtcga
     4141 tatttttcaa cgcctgggag atggcaatct cataaacttt cttcccggta aaccgcgcgc
     4201 gatcatcacc gctcaggcca cgcccggtag cgagaatgac gtaatcggca gcggcaatct
     4261 cttcgctgga taagcggttt tcaacgccac tggacccttg agtttccact tttatggtat
     4321 ggccgagaga acgggctttc tgttccaggg cttccgccac catgtaggtg tgagcgatcc
     4381 cggcggggca gttggtgatc gcgacaatac gtaaggatga ctccatcgcc acttccttat
     4441 gagctgaggg tttgatttaa caaggcaagc acttggtcgg tatcgccctg ttgcagttga
     4501 tgaataaatt ccttgtgaat aattttgcga cacagtgtgc cgatgatttt gacctgatcc
     4561 tcttcgccgc tttgcggcac gccgaggcag atccagcaat tgacgtcttc gccatcgctg
     4621 gctttccagt caatagcctg cgctttgcgg gcgaataata cgaacggttg tttaacgcag
     4681 gcgcttttac cgtgcggcac ggcgacaccg gaaccaaatc cagtagagtg cattttttcg
     4741 cgcagcagca gggtttgcag gaactggtgt gagtcggtga taaaaccgtt ttgtaacgct
     4801 attgtcgcca actgcttcag aacggaataa gcgccattgc cctgaatatt caggtcaata
     4861 cagcttgcag taagagctgc cattaatcgg actccataaa atatatttta atcaattgta
     4921 atggtggagc gattaagtga tgagaggcga tatgtcggta aacaatgaca atttgagaca
     4981 gagtgaaagg tcagatttgc ggagtaatgc acataatggt tatttaaata aaccacatga
     5041 atcattaatg ggttattcat tatttttgtg atttactcac aatgtatcag ggaaatataa
     5101 cttaccggga gatgtaatat gtttaatttt tcaaatcgaa atttaaaata ttgtgccgga
     5161 ggcatctccg gcacattggg caattacggc aggtaaaaca cttcttg
//
LOCUS       DAJTMC010000220         5115 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.220, whole genome shotgun sequence.
ACCESSION   DAJTMC010000220 DAJTMC010000000
VERSION     DAJTMC010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5115
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.220"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(<1..492)
                     /gene="hyfE"
                     /locus_tag="OO130_003869"
     CDS             complement(<1..492)
                     /gene="hyfE"
                     /locus_tag="OO130_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HCR5367086.1"
                     /translation="MIGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHL"
     gene            complement(504..1611)
                     /locus_tag="OO130_003870"
                     /pseudo
     CDS             complement(504..1611)
                     /locus_tag="OO130_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001442177.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit D"
     gene            complement(1623..1733)
                     /locus_tag="OO130_003871"
     CDS             complement(1623..1733)
                     /locus_tag="OO130_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoin racemase"
                     /protein_id="HCR5367087.1"
                     /translation="MENLALTTLMLPFIGALVVSFSPQRRAAEWGFCSPH"
     gene            complement(1750..2697)
                     /gene="hyfC"
                     /locus_tag="OO130_003872"
     CDS             complement(1750..2697)
                     /gene="hyfC"
                     /locus_tag="OO130_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HCR5367088.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLLLTPLFTGISRQIRARMHSRRG
                     PGIWQDYREIHKLFKRQEVAPISSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLATGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            complement(2708..4725)
                     /gene="hyfB"
                     /locus_tag="OO130_003873"
                     /pseudo
     CDS             complement(2708..4725)
                     /gene="hyfB"
                     /locus_tag="OO130_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
     gene            complement(4725..>5115)
                     /gene="hyfA"
                     /locus_tag="OO130_003874"
     CDS             complement(4725..>5115)
                     /gene="hyfA"
                     /locus_tag="OO130_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HCR5367089.1"
                     /translation="LNESLCIGCKLCAVVCPFGAISASGSRPVNAPAQYVFQADGSLK
                     DGEENAPTQHALLRWEPGVQTVAVKCDLCNFLPEGPACVRACPNQALRLITDDSLQRQ
                     MKEKQRLAASWFANGGEDPLSLTQEQH"
BASE COUNT         1393 a         1442 c         1358 g          922 t
ORIGIN      
        1 cagatgggag ccgttttcca tcaggcagta accaaaaatt tgccgcagga tattgcgctg
       61 gctgacaatg cacagcaggc caagcagaaa atgacctaac gctaccgcca gcgccggttt
      121 cagcccggta gccatcggta gcttcacggg ctgaacgaca aatgcgcaaa gcaggacaat
      181 taacgccgcg agcagtgcca tcattgccgg accgaataac gctttttccg ggatgttctg
      241 gggaatattt cgtgcagcgt aagtcatgat taacggtacc agcagcactt tggtgataaa
      301 ggcgctggcg gaccagatca gcagttgctc tgcggcgaac aggcacgaga gagtggcgaa
      361 aatagacacc agcaccagcg actggcaggc gtaaaatccg catgacaggc gatagctttt
      421 gacgctaatc acaaacagcg atgtcagcat catcagtccc gccagattat ttacgatcat
      481 agaaccgatc attcatcatc tccttactgc aaccaggtcg cggcgattac gctggaaatc
      541 agcgacatca caatcagtac aatcaacacc aggcgcattg accctggcag cggtgcggca
      601 tcggcgacgg cctcgctcgg tttgccaggc acaacgcgac caaaccagcg aataaaccag
      661 gcgaaactgg cgaccgattc aatcatcagc agaatcatgg cgggcagcag gatccagtac
      721 tccactgaca acgcaaaacc ggcggcaaac agcgggaatt tactgaagaa gccgttgaac
      781 ggcggcacac cggtaatcgc cagcgctgcc acgcagaaac ccacgcctgg caacggcagg
      841 gagtgcaata cgccacgcag acgcggcaac aagcgcgtgc cgcagctgta actcagcgca
      901 cctgctacaa ggaaaaacag gcttttagcg aacgcgtggt tgacgatgta ggcgatgctg
      961 ccctccagtg ccagccgccc actttcacca tcgatgcggc gtggagatag gctctgatcg
     1021 gtgttggcgc ttccattgcg tccggtagcc acgcttgcat cggtagctgg gccgatttcc
     1081 cccacgcggc aaacaggatg ccgccataaa ccagataacg tgcgtcgcca tgtaactcgc
     1141 tcatcgcgct aagcacaaac gttccggtct gcaaaaacag cgtggcggcg gcaagataca
     1201 accccaacga accgatatgg gtgataagca acgcttttag tgttgaacgc tgcgctttat
     1261 cgctctggta ataactgatc aacgcccagg agcagccgcc ctaatttcaa aaaacaacaa
     1321 ctgaccgagc agcgtcgagg agagtaccag tcccgccatc gcgccgataa acaccagcaa
     1381 aaatgcgtaa taacgattcg tgccgttatg cgggtgttcg cgatttttat ccgtcagata
     1441 acccgtggag tagatcgtga ccagcaaacc gagaaacacc accacaaaca gaatcagcgt
     1501 actcacgcga tcaatgacca ggccaaacaa tgccacatcc cccacgttga ccaacgtcaa
     1561 cgtgacggca actttatcgg cctgataaaa cgcggagatc agcgacaaca tgcacagcgt
     1621 ggtcagtgcg gcgaacaaaa cccccattcg gcggcccgac gttgtggcga aaacgaaacg
     1681 accagtgcgc cgataaaagg cagcattaac gtcgtcagag caagattttc catattccgt
     1741 cagtgctcct tacagaccgg ttaaccagaa gacccatgca agcgcagcaa ggctgaagcc
     1801 aagccaggtc acatggtgaa tgagtaaaaa acgcccgcgt gccagcgtgt tttctgcgat
     1861 tgaggccagt acaaaaatca gcaaaacctt gagcagcgta acgacaagtg aagtcagcag
     1921 gcaggcgaga gaaagttctt gcgcgcgccc aaagggcaga aacagggcca caaacagtgc
     1981 tgccatcacg acctgtttca gccccagccc ccatttcgct agcgccagcc cggcaccgga
     2041 atattcggtc agcgggcctt cctgtaattc ctgttctgct tcagcaacat caaagggaat
     2101 ttttcccatc tcaatgaagc aggcaaaacc acaggccagt aacgccagta cggtggttag
     2161 tggcgagttc cagcccgttg ccagcgtatt gctgatcatc tcgatatgcg tggaacctgc
     2221 tatcagcgcc aataccagca gtgagagaat gagcatgggt tcgaccagaa tgccgagcgt
     2281 caactcgcga ctggcaccga ctcccgcaaa cgggcttccg gtatccagtc cggaaagagc
     2341 aaagaaaaaa cgaaacaggg caagaagata gataagggtg atcagatcgc cgccgcccgc
     2401 aaaaggggaa acggtaataa aaagtggtag ggccatcgcc agcaccagca tgctgctgat
     2461 taatacccac ggcatcaggc ggaacatcag acctgaagat ataggcgcaa cttcctggcg
     2521 tttaaacagt ttgtggatct cgcgataatc ctgccagatc cccggcccgc ggcgggagtg
     2581 catacgcgcg cgtatctgcc gggaaatacc cgtaaaaagc ggggttagca gcagcagaat
     2641 cacggcctgt gctaacgcaa aaatgaccag atatccgtcg caaagagttt gtctcatggt
     2701 gatttcctta gacggcaata gcgattagca gcacaaccag tgcggcaact agatacaggc
     2761 aatagagacg aaagtcgccg ctttgcagat gctgtatttc tttggccagc cgttgggtgg
     2821 cgctcacgat ggggcggatc acccgctcat cccagaaggg ttctgtgctc tgagccctgg
     2881 cggtgacgtg cgcaagatct ttatttagcc tcagcgtagg gtcgagctgt tgtcgtagac
     2941 gaaataacgc agagaagacc acacgcagcg gctgcatcac gccattgcct gacggagcca
     3001 tcgcgttttc ccagccgtag ccgcatgccc aggcatctcc tgtgcgacga aacgctgcgc
     3061 ggcgcgaacg acaccacagc cagtaaaggc caggcagcaa aggcatcgcc agtagtaaca
     3121 gcaacagtaa tgaaggagtg acccgtgtat gaaacgtgcc gggtacaagt gctattccgc
     3181 tggcgacagt gacgggaggg gtattggtaa acgcatgggc aatatgcatt atcttcggtg
     3241 ccagccaact ggcactaagc gcaattaata cgcagagtgc cgcgagcagt agcatggcga
     3301 cgatcatcgt atttggcact tcctgtgcct cttcagcgtg tgtactgcgc ggcgcaccac
     3361 agaaagtaat accgtacatt ttgacgaagc acattactgc cagcccacca gtgactgcca
     3421 gcattaccat agcaatagga cccgcaagtt gtagcgctac ggcttccaca cgacttagtg
     3481 agaacagcga ctgccaggtg taccattcgc tgataaaacc attcagcgga ggaatggctg
     3541 atatcgcgag gcaaccaatc aggcatgctg cggctgtcca cggcatccgt ttcgccagtg
     3601 cccccatttt ttccatgtcg tgggtatgca aacgcgaaat aatcgcgccc gcgccgagaa
     3661 atagcagccc tttgaacagc gcatggttga gcagatgaaa cagtgcgccg agcagtccaa
     3721 ccacggtgag cagcgggtcg tgcagtgaca gaccgtccat cgccacaccg actgccagca
     3781 aaataatgcc gacgttttcg acggtactcc aggccagcag ccgtttgata tcctgttccg
     3841 ccagcgcata tagcacgccc aggagcgcgg agattgcgcc gatcgccatc accagaatgc
     3901 cccaccacag aggcaaaccc gtttgcgcca gcagatccat cgctactttc aggatgccga
     3961 aaataccgat tttgaccatt acgccagaca tcaacgccga agcgtgcgat ggtgcggcag
     4021 ggtgagcgcg cggcaaccag ctgtgcaacg gcatcatccc ggctttcgcg ccaaaaccga
     4081 aaaaggccag cagaaacatc gccgacgcca gccccggaga aagtgaaagc gtgcgaaact
     4141 ggcaaaatcg aggctgccgc tttcgcgccc catcagcaag aaggcgatca taatcagcac
     4201 cgatccggcg tgggcgataa aaaagtagag catgccagcg ttgatcgacg ttttatcctg
     4261 cctggcaatg accagaaacc aggaagacag cgacatcatt tcaaacagca cgatgaacca
     4321 aaaagcgttg tccatcacca gcagggcaac catcgatgcg atgaaaagat tcataaagaa
     4381 gcccatcgcc gccgcgcctt tgccctcgta ttcgcgcata taagtcaatg aatagagcga
     4441 acaaaccacc accagcaagg agataaccag caccataaat accgagaggc tatccattcg
     4501 cagggttaaa tcggcaaagt caaaaggggt ggcaaaacgg gcgactaaag ttacaccagc
     4561 atgtaattgc gtaattccgc tgatgatgcc aatcacgccg cccaccagcg atgtgatgcc
     4621 ggaaagctta atagccagtc tgtccagacc gagtaaaaac agcgaagcca gactggcaaa
     4681 cagaaagagg atcagcgacc aggttaataa ttgcagggca tccattagtg ttgctcctga
     4741 gtgagggaaa ggggatcctc cccgccattg gcaaaccagc ttgcggcaag gcgctgtttt
     4801 tctttcatct gacgttgcag gctatcgtcg gtaatcagcc gtaacgcctg attcgggcaa
     4861 gcgcgaacgc aggccggacc ttctggcaag aaattacaca ggtcgcattt caccgcgacg
     4921 gtctggacac ccggttccca gcgcagcaaa gcatgttggg tcggcgcgtt ttcttcgcca
     4981 tctttgagtg agccgtcagc ctgaaaaaca tattgcgcag gggcattcac cggacggctt
     5041 cctgaagcgc tgattgcgcc aaatgggcag accacggcgc aaagcttgca gccaatacag
     5101 aggctttcgt tgagt
//
LOCUS       DAJTMC010000221         4960 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.221, whole genome shotgun sequence.
ACCESSION   DAJTMC010000221 DAJTMC010000000
VERSION     DAJTMC010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4960
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.221"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(<1..247)
                     /locus_tag="OO130_003875"
     CDS             complement(<1..247)
                     /locus_tag="OO130_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3029 family protein"
                     /protein_id="HCR5367090.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLAN"
     gene            505..1284
                     /gene="deoC"
                     /locus_tag="OO130_003876"
     CDS             505..1284
                     /gene="deoC"
                     /locus_tag="OO130_003876"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HCR5367091.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQTKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            1411..2733
                     /gene="deoA"
                     /locus_tag="OO130_003877"
     CDS             1411..2733
                     /gene="deoA"
                     /locus_tag="OO130_003877"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HCR5367092.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDARALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            2785..4008
                     /gene="deoB"
                     /locus_tag="OO130_003878"
     CDS             2785..4008
                     /gene="deoB"
                     /locus_tag="OO130_003878"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCR5367093.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            4088..4807
                     /gene="deoD"
                     /locus_tag="OO130_003879"
     CDS             4088..4807
                     /gene="deoD"
                     /locus_tag="OO130_003879"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCR5367094.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGIDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
BASE COUNT         1193 a         1265 c         1363 g         1139 t
ORIGIN      
        1 cgttcgccag aaaacgggcg taatcgggta agacatagcg cggtttgtac ggcgcatgac
       61 cttcaaacat atcgcagatt acaccctcat ccagcgcgcg gcgggcttcg gcaggaagct
      121 gcggataagg cagattgttt tctgcttcca gagccagaaa atggcgcttc tgctccgggc
      181 taagcactgg gctggtgaca atttgctggc aacgttgttg cagtgcattt tcatgagaag
      241 tgggcatctt cttttccttt tatgccgaag gtgatgcgcc attgtaagaa gtttcgtgat
      301 gttcactttg atcctgatgc gtttgccacc actgacgcat tcatttgaaa gtgaattatt
      361 tgaaccagat cgcattacag tgatgcaaac ttgtaagtag atttccttaa ttgtgatgta
      421 tatcgaagtg tgttgcggag tagatgttag aatactaaca aactcgcaag gtgaatttta
      481 ttggcgacaa gccaggagaa tgacatgact gatctgaaag caagcagcct gcgtgcactg
      541 aaattgatgg acctgaccac cctgaatgac gacgacaccg acgagaaagt aattgctctg
      601 tgtcatcaga ccaaaactcc ggtcggcaat accgccgcta tctgtatcta tcctcgcttt
      661 atcccgattg ctcgcaaaac gctgaaagag cagggcaccc cggaaatccg aatcgctacg
      721 gtaaccaact tcccacacgg taacgatgac atcgaaatcg cgctggcaga aacccgtgcg
      781 gcaatcgcct acggtgctga tgaagttgac gttgtgttcc cgtaccgcgc gctgatggcg
      841 ggtaacgagc aggttggttt tgacctggtg aaagcctgta aagaggcttg cgcggcagcg
      901 aatgtactgc tgaaagtgat catcgaaacc ggcgaactga aagacgaagc gctgatccgt
      961 aaagcgtctg aaatctccat caaagcgggt gctgacttca tcaaaacctc taccggtaaa
     1021 gtggcagtga acgcgacgcc ggaaagcgcg cgcatcatga tggaagtgat ccgtgatatg
     1081 ggcgtagaaa aaaccgttgg tttcaaaccg gcgggcggcg tgcgtactgc ggaagatgcg
     1141 cagaaatatc tcgccatcgc agatgaactg ttcggtgctg actgggcaga tgcgcgtcac
     1201 taccgctttg gtgcttccag cctgctggca agcctgttga aagcgctggg ccacggtgac
     1261 ggtaagagcg ccagcagcta ctaagcaaga tgctttacgc ctgatgcgct gcgcttatca
     1321 ggcctacgag acgtatctac ccgtaggccg gataaggcgt agacgcatcc ggcaaaagcc
     1381 gcctcatact cttttcctcg ggaggttacc ttgtttctcg cacaagaaat tattcgtaaa
     1441 aaacgtgatg gtcatgcgct gagcgatgaa gaaattcgtt tctttatcaa cggcattcgc
     1501 gacaacacta tctccgaagg gcagattgcc gccctcgcga tgaccatttt cttccacgat
     1561 atgacaatgc ctgagcgtgt ctcgctgacc atggcgatgc gagattcagg aaccgttctc
     1621 gactggaaaa gcctgcatct gaatggcccg attgttgata aacattccac cggcggcgtc
     1681 ggcgatgtca cttcgctgat gttggggccg atggtcgcag cctgcggcgg ctatattccg
     1741 atgatctccg gtcgcggcct cggtcatact ggcggtacgc tcgacaaact ggaatccatc
     1801 cctggcttcg acattttccc ggatgacaac cgtttccgcg aaattattaa agacgtcggc
     1861 gtggcgatta tcggtcagac cagctcactg gctccggcgg ataaacgttt ctacgcgacc
     1921 cgtgatatta ccgcaaccgt agactccatc ccgctgatca ctgcctcgat cctggcgaag
     1981 aaactggcgg aaggtctgga tgcgctggtg atggacgtga aagtgggtag cggcgcgttt
     2041 atgccgacct acgaactctc tgaagccctt gccgaagcga ttgttggcgt ggctaacggc
     2101 gctggcgtgc gcaccaccgc gctgctcacc gacatgaatc aggtactggc ctccagtgca
     2161 ggtaacgcgg ttgaagttcg tgaagcggtg cagttcctga cgggtgaata tcgtaacccg
     2221 cgtctgtttg atgtcacgat ggcgctgtgc gtggagatgc tgatctccgg caaactggcg
     2281 aaagatgacg ccgaagcgcg cgcgaaattg caggcggtgc tggacaacgg taaagcggca
     2341 gaagtctttg gtcgtatggt agcggcacaa aaaggcccga ccgacttcgt tgagaactac
     2401 gcgaagtatc tgccgacagc gatgctgacg aaagcagtct atgctgatac cgaaggtttt
     2461 gtcagtgaaa tggatgcccg cgcgctgggg atggcagtgg ttgcaatggg cggcggtcgt
     2521 cgtcaggcat ctgacaccat cgattacagc gtcggcttta ctgatatggc gcgtctgggc
     2581 gaccaagtag acggtcagcg tccgctggcg gttatccacg cgaaagacga aaacagctgg
     2641 caggaagcgg cgaaagcggt gaaagcggca attaaacttg ccgataaagc accggaaagc
     2701 acaccaactg tctatcgccg tatcagcgaa taacggtata ctgatctgat catttaaatt
     2761 tgaagcactg agtacggaga acatatgaaa cgtgcattta ttatggtgct ggactcattc
     2821 ggcatcggcg ctacagaaga tgcagaacgc tttggtgacg tcggggctga caccctgggt
     2881 catatcgcag aagcttgtgc caaaggcgaa gctgataacg gtcgtaaagg cccgctcaat
     2941 ctgccaaatc tgacccgtct ggggctggcg aaagcacacg aaggttctac cggtttcatt
     3001 ccggcgggaa tggacggcaa cgctgaagtt atcggcgcgt acgcatgggc gcacgaaatg
     3061 tcatccggta aagatacccc gtctggtcac tgggaaatcg ccggtgtccc ggttctgttt
     3121 gagtggggat acttctccga tcacgaaaac agcttcccgc aagagctgct ggataaactg
     3181 gtcgaacgcg ctaatctgcc gggttacctc ggtaactgcc actcttccgg tacggtcatt
     3241 ctggatcagc tgggcgaaga gcacatgaaa accggcaagc cgattttcta tacctccgct
     3301 gactccgtgt tccagattgc ctgccacgaa gaaacctttg gcctggataa actctacgaa
     3361 ctgtgcgaaa tcgcccgtga agagctgacc aacggcggct acaacatcgg tcgtgttatc
     3421 gctcgtccgt ttatcggtga caaagccggt aacttccagc gtaccggtaa ccgtcacgac
     3481 ctggctgttg agccgccagc accgaccgtg ctgcagaaac tggttgatga aaaacacggt
     3541 caggtggttt ctgtcggtaa aattgcagac atctacgcca actgcggtat caccaagaaa
     3601 gtgaaagcaa ctggcctgga cgcgctgttt gacgccacca tcaaagagat gaaagaagcg
     3661 ggtgataaca ccatcgtctt caccaacttc gttgacttcg actcttcctg gggccaccgt
     3721 cgcgacgtcg ccggttatgc tgcgggtctg gagctgttcg accgccgtct gccggagctg
     3781 atgtctttgc tgcgcgatga cgacatcctg atcctcactg ctgaccacgg ttgtgatccg
     3841 acctggaccg gtactgacca cacgcgtgaa cacattccgg tactggttta cggcccgaaa
     3901 gtaaaaccgg gttcactggg tcaccgtgaa accttcgcgg atatcggcca gactctggca
     3961 aaatattttg gtacttctga tatggaatat ggcaaagcca tgttctgata atgggtgcgg
     4021 cttgatgccc tcaccccggc cctctcccac agggagaggg tgaaaacatg ttgataagga
     4081 taaaacaatg gctaccccac acattaatgc agaaatgggc gatttcgctg acgtagtttt
     4141 gatgccaggc gacccgctgc gtgcgaagta tattgctgaa actttccttg aagatgcccg
     4201 tgaagtgaac aacgttcgcg gtatgctggg cttcaccggt acttacaaag gccgcaaaat
     4261 ttccgtaatg ggtcacggta tgggtatccc gtcctgctcc atctacacca aagaactgat
     4321 caccgatttc ggcgtgaaga aaattatccg cgtgggttcc tgtggcgcag ttctgccgca
     4381 cgtaaaactg cgcgacgtcg ttatcggtat gggtgcctgc accgattcca aagttaaccg
     4441 catccgtttt aaagatcatg actttgccgc tatcgctgac tttgacatgg tgcgtaacgc
     4501 ggtagacgcg gctaaagcac tgggcattga tgctcgcgtt ggtaacctgt tctccgctga
     4561 cctgttctac tctccggacg gcgaaatgtt cgacgtgatg gaaaaatacg gcatcctcgg
     4621 cgtggaaatg gaagcggctg gtatctacgg cgtcgctgca gaatttggcg cgaaagccct
     4681 gaccatctgt accgtgtctg accacatccg cactcacgag cagaccactg ccgctgagcg
     4741 ccagaccacc ttcaacgaca tgatcaaaat cgcactggaa tccgttctgc tgggcgataa
     4801 agagtaattg tgtttcgctg aaaggcgatt gtcgtgtgaa gccggagtgg gagactgctc
     4861 cggcttttta gtatctattc atcaatagca actgatttat agatttacta ttatttttca
     4921 tatggataga aaataaaatc tattcaagta tctattcacg
//
LOCUS       DAJTMC010000222         4873 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.222, whole genome shotgun sequence.
ACCESSION   DAJTMC010000222 DAJTMC010000000
VERSION     DAJTMC010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4873
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.222"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            300..1274
                     /gene="hemB"
                     /locus_tag="OO130_003880"
     CDS             300..1274
                     /gene="hemB"
                     /locus_tag="OO130_003880"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HCR5367095.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(1380..2231)
                     /gene="tauD"
                     /locus_tag="OO130_003881"
     CDS             complement(1380..2231)
                     /gene="tauD"
                     /locus_tag="OO130_003881"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HCR5367096.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGITAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRVTILGD
                     KPFYRAG"
     gene            complement(2246..3055)
                     /gene="tauC"
                     /locus_tag="OO130_003882"
     CDS             complement(2246..3055)
                     /gene="tauC"
                     /locus_tag="OO130_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HCR5367097.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVSPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTP"
     gene            complement(3052..3819)
                     /gene="tauB"
                     /locus_tag="OO130_003883"
     CDS             complement(3052..3819)
                     /gene="tauB"
                     /locus_tag="OO130_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HCR5367098.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQVLKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            complement(3832..4794)
                     /gene="tauA"
                     /locus_tag="OO130_003884"
     CDS             complement(3832..4794)
                     /gene="tauA"
                     /locus_tag="OO130_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5367099.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQAGNT
                     FAKESGASVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKLGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTAELTGPVNKVIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
BASE COUNT         1146 a         1407 c         1231 g         1089 t
ORIGIN      
        1 acgcgttata aatatcaaaa acacaaacca gataaatatc ttatcaatat acaacaaagt
       61 ttgaattatt caatttatag cagcacaacc aaacgcaaaa aaaaaaataa taaccaagag
      121 ctataaataa agattaaaaa tattttcagt gaatatgaac ttattgctat ttctattaca
      181 aagatgtcct ggtctctctt tcattctctt cttgtcaaca tcacgacaac tttcgtaaaa
      241 cactcctgcc ccgcttcagg tatactgtgc accttaattc cacaaacatc aggcagacca
      301 tgacagactt aatccaacgc cctcgtcgcc tgcgcaaatc tcctgcgctg cgcgctatgt
      361 ttgaagagac aacacttagc cttaacgacc tggtgttgcc gatctttgtt gaagaagaaa
      421 ttgacgacta caaagccgtt gaagccatgc caggcgtgat gcgcattcca gagaaacatc
      481 tggcacgcga aattgaacgc atcgccaacg ccggtattcg ttccgtgatg actttcggca
      541 tctctcacca taccgatgaa accggcagcg acgcctggcg ggaagatgga ctggtggcgc
      601 gtatgtcgcg catctgcaag cagactgtgc cagaaatgat cgtcatgtca gacacttgtt
      661 tctgtgaata cacttctcac ggtcactgcg gtgtgctgtg cgagcatggc gtcgacaacg
      721 acgcgactct ggaaaattta ggcaagcaag ccgtggttgc agctgctgca ggcgcagact
      781 tcatcgcccc ttccgccgcg atggacggcc aggtacaggc gattcgtcag gcgctggacg
      841 ctgcaggctt taaagatacg gcgattatgt cgtattcgac caagttcgcc tcttcctttt
      901 atggcccgtt ccgtgaagct gccggaagcg cattaaaagg cgaccgcaaa agctatcaga
      961 tgaaccctat gaaccgtcgc gaggcgattc gtgagtcact gctggatgaa gcccagggcg
     1021 cagactgcct gatggttaaa cctgctggag cgtacctcga catcgtgcgt gagctgcgtg
     1081 aacgtactga attgccgatt ggcgcgtatc aggtgagcgg tgagtacgcg atgattaagt
     1141 tcgccgcgct ggcgggtgct atagatgaag agaaagtcgt gctcgaaagc ttaggttcaa
     1201 ttaagcgtgc gggtgcggat ctgattttca gctactttgc gctggatttg gctgagaaga
     1261 agattctgcg ttaatttttt tcctctcaga aagggggagt taacctgttg aaagtacggt
     1321 gaacgccccc tcaccctaac cctctcccca agggggcgag ggaaccgtcc gcactcgtat
     1381 caccccgccc gataaaacgg tttatcccca agaatcgtca cccgatgcat aatccgccgc
     1441 tgtggcaggt aatcggcatt ggcatagtgc tgagtcacgc ggttatccca aatcgcaata
     1501 tcattcggtt gccagcgcca acgcacctga aactccggtt tagtgatatg ggcgaataaa
     1561 aagcctaaca aggcttcgct ctctttctcg ctgacatcga caattcgcgt agtaaagcct
     1621 tcattcacaa acagcgcctg tttaccgctc accggatgcg ttcgcaccac cggatgtagc
     1681 aacggaggat ttttcgcgac cgcctcgcgc cagcgttgat gttcctcctc ggttttgcgg
     1741 tatttgtatt ccgggaacga tttacggaaa tcatgctccg cacgcagccc gctcagcagc
     1801 tggcggaagg gaacagagag cgcctcatag gccgtaatac cgctggtcca gagcgtatca
     1861 ccgccggtcg aaggtaattc tttagctgcc agaatcgccc ctgcgggtgg cgtttcaata
     1921 aatgtcacat cggtatgcca gttgtcgtta tctggcggat tatcgttatg ggtatccagc
     1981 acgatgatct cgtcaacccc ttcggcatga gggtaaacag ggtgaatatg cagttcgcca
     2041 aaacgctggg ccagcgcgcg ttgctgctgc ggcgtaatgg cttgatcgcg cagaaacacc
     2101 acctgatggc gcagcaccgc atggtaaagc tgttcaaact gattatcgct taacgggcgc
     2161 gtcaggtcgg cacccgaaat ttgtgcgccg atatacggcc ccagcggggt aatgctcaga
     2221 cgttcactca ttgtacttct ccatgctagg gcgtcagacg gcgctgtaac gcgcgcaaac
     2281 ccagttctaa aagaaaggcg ataatcgcaa tcaccgcgat tcccgccagc accacgtcag
     2341 tcgcgagaaa ttcacccgct gactgaacca taaatcctaa accgcgcgtc gcggcaatca
     2401 gctccgccgc taccagcgta gaccagccca cacccagacc aatgcgtaat ccggtgagga
     2461 tttccggcag cgcgccgggc aaaatgacaa accacagcac ctgcgcacgg ctggcaccca
     2521 gcgactgggc ggcacgaatg cgaacctgct gcgcgctttt cacccccgcc agcgccgaca
     2581 tcgccaccgg tgcaaaaatc gctaaataga tcagtaagat cttcgaggtt tcaccaatac
     2641 caaaccagat caccatcagc ggcaaataag ccagcggcga caccggacga taaagttcga
     2701 ttaccggatc cagaatgccg cgcaccgtcg ggcttagccc catggcgatc ccgaccggaa
     2761 taccgatcac caccgccgcg agtagcgcca gcacaatgcg cgtcaggctg gctgccagat
     2821 gctgccacag cgtggcgtcc ataaatccct gcggtccggc aatggtgagc agttttgcca
     2881 gtacttgttg cggcggcggc aaaaacagcg ggctaatcag ttgcagcgcc gccaccgtcc
     2941 accatacagt gagtaaaacc gctaacgtgc caatgcttaa ggtcacctga cgcgagagcg
     3001 gccagcgcca tttcagccgc cgcgaatgca gtttttcatt aatgagcaca ctcatgagaa
     3061 cgcctcccgt tgctcaaata cgcggcttaa aacatattcg cgcatggcga tgaattgtgg
     3121 atcggacttg atgctgcggc tcgactctcc cgcaacaaag cggcgagcaa agttgagcgg
     3181 cagccgctcc agcacacggc cagggccgga tgaaagcaga accagttcag tcgccataaa
     3241 caccgcttct tctatatcgt gggtaatcaa cagcacctgc ttgcccgtct cctgccagag
     3301 tttcagcagc agagtttgca tctggtcgcg ggtgaaggcg tccagcgcac caaacggttc
     3361 gtcgagtaat aacagctggg gattcgccgc cagcgcgcgg gcaatcccca cccgctgacg
     3421 ttgaccaccg gaaagctgcc agatatagcg tttttctgcg ccttccagcc ccactttttt
     3481 cagcacctgg tgcgcgattt ccagtcgctg cattttctct atacctgcca attgcaggcc
     3541 gaacgccacg ttgtcctgta cattgcgcca cggtagcagc ccttcattct gaaaaaccac
     3601 cccgcgctct gctcccggtc cctcaatacg cttacccgcc agttgaatgc tgccatgctg
     3661 ataaggcaca aaaccggcaa tcagattcag caacgtggtt ttaccgcagc cggacggccc
     3721 cagcaccacc agtagctcgc cgctttccag cgtcaggttg atatcttcca gtgccggttt
     3781 gccgccataa tcggcataga gatgagagat ttgcagcatc tgcgcctcct tttattgcac
     3841 gaagcgcgag gtaacgtact ggctgtaatc attcgctaca gccgggacct tgccctgctc
     3901 tttcaaaaac tgcgcggtgt cgatgattac tttgttcacc ggtccggtca gttctgccgt
     3961 ttgttgctgc ggcgtcagat aggtattccc cttaaccagc cccggaacgt caccttccgg
     4021 cacgccgctt aaacgcgcca gtttgctgat gttttccggc tgtttcagcc acgcgtctgg
     4081 gttagcaatg tacggttgct gagcatcgat ggcgctttta gcgaacgctt tcacgacctc
     4141 aggatgtttc tcggcaaaat ctttgcgcac cacccagacg tccagcgttg gcgcacccca
     4201 ctgcccgacc tgttcagaat cggtcaacac cttgccgtct ttttccaggg cgttaaccgc
     4261 cggtgcccag acataagcac catcaatatc accccgctgc catgcggcga taatcgcggg
     4321 cggctgcagg ttcacaatct ccacttgccc tagtttaatc ccccagtgtt tcagcgccgc
     4381 cagcaggctg tagtgggtgg tggagataaa cggtacggca atgcgtttgc caatcaaatc
     4441 ttcgggtttg ctgatagttt tctttaccac cagcgcttcg gagttaccca gttttgacgc
     4501 cagcaagaag acttcaatcg gcacctgttg gctggctgca accgctaatg ggctggaacc
     4561 gaggttgccg atttgcacgt cgccagaagc cagcgcccgc acgatgctgg ctccgctgtc
     4621 aaacttacgc cagtccacgc tcgccccgct ctctttagca aaggtgttgc cggcctgagc
     4681 cactttcgcc ggttcggctg aggtttgata cgccacggtg acgttcaccg cctgtgcctg
     4741 aaaagcgatg aatgccagtg cggcaagaag tgtgttacgc gatgaaattg ccatgattgt
     4801 ctgctcccca gtcttgttat gggagcagta ttcaggaata aaaacattca ttaaaagaat
     4861 tagtcgttat cgc
//
LOCUS       DAJTMC010000223         4862 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.223, whole genome shotgun sequence.
ACCESSION   DAJTMC010000223 DAJTMC010000000
VERSION     DAJTMC010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4862
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.223"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            148..264
                     /locus_tag="OO130_003885"
     CDS             148..264
                     /locus_tag="OO130_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004996477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR5367100.1"
                     /translation="MPGIPPEIRWNNQKNALQLWNKEGGVVRREGDPLEMAQ"
     gene            628..1470
                     /locus_tag="OO130_003886"
     CDS             628..1470
                     /locus_tag="OO130_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCR5367101.1"
                     /translation="MLNEGGILFKANHVPVLMYHHVSHCPGLVTLSPVTFRKQMKWLA
                     ENNWKTLSSDELEFFYRGGKLPRKSVMLTFDDGYLDNWFQVYPLLKEFNLKAHIFLIT
                     GFIGNGPVRHSPGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTH
                     TRWDKKFSSREEQCKHLRQDLLSGREYLKEMTGKCSKHLCWPEGYYNKDYIQVAEELG
                     FYYLYTTERRMNAPAKGTTRIGRISTKERESCAWLKRRLFYYTTPFFSSLLAFHKGPR
                     LPDD"
     gene            1473..2561
                     /gene="capU"
                     /locus_tag="OO130_003887"
     CDS             1473..2561
                     /gene="capU"
                     /locus_tag="OO130_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HCR5367102.1"
                     /translation="MNILFTESSPNIGGQELQAVAQMKALKKMGHSVLLVCRENSKIA
                     FEASKLGIDITFALFRNSLHIPTAWRLLGIVHGFQPNAIVCHSGHDSNIVGLVRLFTW
                     KHPFRIIRQKTYLTQKTKVFSINHFCDEVIVPGTSMKTHLEQEGCRTRVTVVPPGFDF
                     QKLYVDSRNSLPPNVLSWLASRRGCPVIAQVGMLRPEKGHEFMLNLLFHLKMNGRQFC
                     WLIVGSGSPELREHLQYQIDSMGMHDDVFIADNVFPAAPVYRVASLVVLPSENESFGM
                     VLAEASAFSVPVLASQIGGIPDVIQNNQTGTLLPAGNKHAWMCALNDFFNDPGRFYQM
                     ARQAKQDIEERFDINKTALKILTLAKHK"
     gene            2566..3516
                     /gene="virK"
                     /locus_tag="OO130_003888"
     CDS             2566..3516
                     /gene="virK"
                     /locus_tag="OO130_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HCR5367103.1"
                     /translation="MFSVSNLSFIGFLKRIVFSSDSLPGKWEHRKFRFMYILRCAINP
                     VASIRYYYELRSLPCIEDILAIHPTLPARIHRPYLHKGGRAWTRGQYILEHYRFVQNL
                     PEKYSKFLFPQKSVSLVQFIGKDGENFDIQCSSSGFDREGELMLSLFYNKTVITRLTF
                     SVILTQNGHIAFIGGLQGAPKNTGPDVIRCATRACYGIFPKRIIFEAFCALMKACNIS
                     ECLAVSEHSHVFRQLRYWYQKRKTFVAVYSDFWESVAGKTCGDWYRLPTQVIRKPLSD
                     IASKKRSEYRKRYALLDYIHETTIRSLDAYPVHSEHQDLN"
     gene            3581..4525
                     /gene="lpxM"
                     /locus_tag="OO130_003889"
                     /gene_synonym="msbB"
     CDS             3581..4525
                     /gene="lpxM"
                     /locus_tag="OO130_003889"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCR5367104.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWSTWFLLGMIAGISMFPPSFRDP
                     VLAKIGRWAGRLSKKARRRATINLSLCFPEKSDTEREIIVDKMFATALQSIVMMAELA
                     IRGPEKFQKRVFWKGLEILEEIRYNNRNVIFLVPHGWSVDIPAMLLAAQGEKMAAMFH
                     QQRNPVIDYVWNSVRRKFGGRLHSREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEFA
                     DFFATYKATLPIIGRLMNISQAMIIPLFPVYDEKKHFLTIEVRPPMDACIASADNKMI
                     ARQMNKTVEILVGSHPEQYIWVLKLLKTRKSNEADPYP"
     gene            complement(4679..>4862)
                     /locus_tag="OO130_003890"
     CDS             complement(4679..>4862)
                     /locus_tag="OO130_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HCR5367105.1"
                     /translation="IYQPVYSPWVNHAERLWQALHDTITRNHQCRSMWQLLKKVRHFM
                     ETVSPFPGGKHGLAKV"
BASE COUNT         1386 a          923 c         1116 g         1437 t
ORIGIN      
        1 tatcggtggc aatagcctgt gcaaattttt tataacccgg gatcgtgtca ggattcagag
       61 tatgataaag acagattttc atggctgccg gtttccgtcg tgcaaaaaac atgctgattt
      121 tacgaaggaa tgccttactg aacaaccttg cccggaatac cccccgaaat cagatggaac
      181 aatcagaaaa atgctctgca gttatggaat aaagaaggtg gggtggtacg ccgggagggc
      241 gatccgctgg aaatggcgca gtagctgcag gcctgtcatg atgccggaat agaagttcgt
      301 gtacaggtta atgaaagcgt caccccgtaa ataaactctg cgctgaaatt cctttttttc
      361 attggagcac ctgtgttgtt ctgaggtgaa catatcacct ctgttcaggt agccaaattc
      421 agtgtgccac ctcaaggttc ttagtgagtc gtttttaaaa gatatgataa tatatcattg
      481 tttatgaatt gttgatgttt aatttagtaa aatatatcaa gtatgccatg ggtttgctat
      541 gttattcatc cagtatctgg aaataaaatg caccagcttt attgggtttt ccttttgtct
      601 ctgcttacta ggtgccagaa actttaaatg ttaaacgaag ggggcatttt gttcaaagcc
      661 aatcatgttc cggtattaat gtatcatcat gtcagtcatt gccccggact ggttacactt
      721 tctcccgtga cattccggaa gcaaatgaaa tggttggctg aaaataactg gaaaacactg
      781 tcatcagatg aactggagtt tttttatcgg ggcggaaaac tcccccggaa aagcgttatg
      841 ttgacatttg atgatggata ccttgacaac tggtttcagg tatatccttt attaaaagag
      901 tttaatctca aagcacatat ttttttaatt accggtttta ttgggaatgg tccggttcgt
      961 cactctcctg gaaaggaata ttctcatcgc gattgcgaac atcaaatagc gacagggaat
     1021 gctgataatg ttatgcttcg ctggtctgaa gtaaacgaaa tgttgcaatc tggtctggtc
     1081 gaatttcatg ttcatacgca tactcataca cgctgggata aaaagttcag ttcacgagaa
     1141 gaacagtgta aacatcttcg gcaggacttg ttgagtggga gagaatattt gaaagaaatg
     1201 acaggaaaat gcagtaaaca tttgtgttgg ccggaaggtt attataacaa ggattatatt
     1261 caggtcgctg aagaacttgg attctattat ttatatacaa cagaaagaag aatgaatgct
     1321 cctgctaaag gaactacacg gataggaaga ataagcacca aagaacggga aagttgcgca
     1381 tggctgaaaa gacgactttt ttattataca acaccgtttt tttcatcgtt gctggccttt
     1441 cataaggggc caagattacc ggatgattaa taatgaatat actatttacg gaatcatcac
     1501 cgaatatagg tggtcaggaa ttacaggctg ttgctcaaat gaaggccctg aagaaaatgg
     1561 ggcattcagt tctgcttgtc tgcagggaaa acagcaaaat tgcttttgaa gccagtaaat
     1621 tgggaattga tatcacattc gcgttatttc gaaacagtct tcacatccct actgcatgga
     1681 gattactcgg aatagttcat ggttttcagc ccaatgcaat cgtttgtcac agtgggcatg
     1741 atagcaatat tgttggttta gtacggttat tcacttggaa acatccattc agaattatca
     1801 ggcaaaagac atatttgaca caaaaaacaa aagttttttc aataaatcat ttttgcgatg
     1861 aggtgattgt tcccggaaca agtatgaaga cacatctgga gcaggaagga tgtcgaaccc
     1921 gggttactgt tgtgcctcca ggctttgatt tccagaaatt atacgttgat tcccgaaaca
     1981 gtttgcctcc aaatgttctt tcttggctgg cgtcccgaag gggatgcccc gttattgctc
     2041 aggtaggaat gttgcgcccg gaaaaagggc acgaatttat gttgaattta ctgttccatt
     2101 taaaaatgaa tggacgacag ttctgttggt tgattgtggg gtctggttcg cctgaactgc
     2161 gggagcattt acagtatcag attgacagta tgggcatgca tgatgatgtt tttattgctg
     2221 acaatgtttt tcctgccgcc cccgtatatc gggttgccag tctggtggtt ctgccttcag
     2281 aaaacgaatc gtttggtatg gtactggcag aagcatcggc attttctgtg cctgtactgg
     2341 ccagtcagat tggtggaatc cctgatgtta ttcagaacaa ccagaccggg acattgttac
     2401 cagcaggtaa taagcacgca tggatgtgcg ccctgaatga tttttttaat gaccctgggc
     2461 gtttttatca gatggctcgc caggcaaaac aggatataga agagcggttt gatattaata
     2521 aaactgcgtt aaaaatactc acattagcga agcacaagta acaatatgtt ttctgtaagt
     2581 aacttatcat ttatcggttt ccttaaaagg attgtttttt cctcagattc actcccgggg
     2641 aagtgggaac acagaaaatt ccggttcatg tacattttgc gatgtgctat aaatccggtt
     2701 gccagtattc gatattatta cgaactgcgt tccttgccgt gcattgagga tattctggca
     2761 atacacccca cgttgccagc aagaattcat cgcccttatt tgcataaagg ggggcgcgcc
     2821 tggacccgtg gacaatatat tcttgagcat tatcgctttg tgcaaaattt gccagaaaaa
     2881 tattctaaat ttttgtttcc acaaaaatcg gtatcattag tccaattcat cgggaaagat
     2941 ggagagaatt ttgatatcca gtgttcttcc agtggttttg accgggaagg agagttgatg
     3001 ttgtcattgt tttacaataa aacagttatt acccggttaa cattttcagt cattctgact
     3061 caaaatggac acattgcttt tattggcgga ttacagggag cccctaaaaa taccggacct
     3121 gatgttattc ggtgcgcaac ccgggcctgt tatgggattt tcccaaaacg gattattttt
     3181 gaagcatttt gtgcgttgat gaaagcttgc aatatctctg agtgtctggc agtcagtgag
     3241 cacagtcatg ttttccggca attgagatac tggtatcaga aacgcaaaac ctttgttgcg
     3301 gtctatagtg atttctggga gtctgtagca ggaaaaacct gtggtgactg gtacagatta
     3361 ccaacccagg tgattcgaaa gccgctaagc gatatagcca gtaaaaaacg ctctgagtac
     3421 cgaaaacgat acgctttgct ggattatatc catgaaacta ctatccgctc tttggatgca
     3481 tatcctgtac actcagaaca tcaggattta aattaaggtt tgatgtattc tctgaataaa
     3541 acagtaatga taattttggt agaggcattc gcactaaata atgaaaaaat ataaatctga
     3601 gtttattcct gaatttaaga aaaattatct ttcccctgtt tactggtcta catggttcct
     3661 tttgggaatg attgcaggta tttcaatgtt tcccccttca ttcagagatc ctgtcttggc
     3721 aaaaataggg cgttgggcgg gcagattgag caaaaaagct cgtcgcaggg cgacgattaa
     3781 tttatcgctt tgtttcccag aaaagagtga tacagaacgg gaaataattg tcgacaaaat
     3841 gtttgccaca gcattgcaat ccatagtgat gatggcagaa ctggcaattc gtggtccgga
     3901 aaagttccag aaacgagtgt tctggaaagg cctcgaaatt cttgaggaaa ttcgatacaa
     3961 taacagaaat gtgatttttc tggttcccca tggctggagc gtggatattc ctgcaatgtt
     4021 gctggcagct cagggggaaa aaatggccgc catgtttcat cagcaacgaa atccagtgat
     4081 tgattatgtc tggaattctg tacggcgtaa attcgggggg cgcttacatt cccgggagga
     4141 tgggataaaa ccatttattc agtcagtacg ccagggatac tgggggtatt accttccaga
     4201 tcaggatcat ggtcctgaat acagtgaatt tgctgatttt tttgcgacct ataaagcgac
     4261 attaccaatt attggacgtc tgatgaacat cagtcaggct atgattatac cgcttttccc
     4321 ggtttatgat gaaaaaaaac atttcctgac tattgaagtt cggccaccaa tggatgcatg
     4381 cattgccagc gcggacaata aaatgattgc ccgacaaatg aacaaaacag tggaaatttt
     4441 ggtggggtca catccggaac agtatatctg ggttttaaaa ttgttaaaaa cgcgcaaatc
     4501 aaacgaagcg gacccgtacc cttgaaatta taaagtacag gtatttccac tagttgtttc
     4561 ttacaggtta ccaatcgaaa cacatccccc ttccgtgtgt tgcccgtttg gcacggagga
     4621 ctgtgtagat gcagttcgtt aaatttaatt tatgcctaaa tagctgcgct taataccgct
     4681 acacttttgc cagtccatgt ttgcctccgg ggaatgggct gacggtttcc ataaaatggc
     4741 gaactttttt caacagttgc cacattgagc ggcactgatg attacgcgtt attgtgtcgt
     4801 gaagtgcctg ccatagccgt tcagcatgat tcacccatgg cgagtaaacc ggctgataaa
     4861 tg
//
LOCUS       DAJTMC010000224         4852 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.224, whole genome shotgun sequence.
ACCESSION   DAJTMC010000224 DAJTMC010000000
VERSION     DAJTMC010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4852
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.224"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(<1..1250)
                     /locus_tag="OO130_003891"
     CDS             complement(<1..1250)
                     /locus_tag="OO130_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AGCS family amino acid carrier protein"
                     /protein_id="HCR5367106.1"
                     /translation="MPDFFSFINSVLWGSVMIYLLFGAGCWFTFRTGFVQFRYIRQFG
                     KSLKNSIHPQPGGLTSFQSLCTSLAARVGSGNLAGVALAITTGGPRAVFWMWVAAFIG
                     MATSFAECSLAQLYKERDVNGQFRGGPAWYMARGLGMRWMGVLFAVFLLIAYGIIFSG
                     VQANAVARALSFSFDFPPLVTGIILAVFALLAITRGLHGVARLMQGFVPLMAIIWVLT
                     SMVICVINIGQLPHVIWSIFESAFGWQEAAGGAAGYTLSQAITNGFQRSMFSNEAGMG
                     STPNAAAAAASWPPHPAAQGIVQMIGIFIDTLVICTASAMLILLAGNDTTYMPLEGIQ
                     LIQKAMRVLMGSWGAEFVTLVVILFAFSSIVANYIYAENNLFFLRLNTPKAIWCLRIC
                     TFATVIGGTLLSLPLMWQLADIIMA"
     gene            1529..2482
                     /gene="tal"
                     /locus_tag="OO130_003892"
     CDS             1529..2482
                     /gene="tal"
                     /locus_tag="OO130_003892"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCR5367107.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPTLLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            2597..3184
                     /gene="mog"
                     /locus_tag="OO130_003893"
     CDS             2597..3184
                     /gene="mog"
                     /locus_tag="OO130_003893"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HCR5367108.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSVLTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAAGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(3219..3785)
                     /gene="satP"
                     /locus_tag="OO130_003894"
     CDS             complement(3219..3785)
                     /gene="satP"
                     /locus_tag="OO130_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HCR5367109.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YSGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(3934..4647)
                     /gene="msyB"
                     /locus_tag="OO130_003895"
     CDS             complement(3934..4647)
                     /gene="msyB"
                     /locus_tag="OO130_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HCR5367110.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAFDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            complement(4673..>4852)
                     /locus_tag="OO130_003896"
     CDS             complement(4673..>4852)
                     /locus_tag="OO130_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HCR5367111.1"
                     /translation="ARSASQSLNIPSEIKEGQTTDWININSDNDNKRCVSKITFSGHT
                     VNSSDMATLKIIGDD"
BASE COUNT         1275 a         1249 c         1229 g         1099 t
ORIGIN      
        1 gccattatga tatctgccag ttgccacatc agcggaaggc ttagcaaggt gccgccgatg
       61 accgttgcga aggtgcagat ccgcaaacac cagatcgctt taggggtgtt caggcgtaaa
      121 aagaagagat tgttttcggc ataaatgtag ttggcaacga tggagctgaa ggcaaacaga
      181 ataaccacga gggtaacaaa ctcagcaccc caggaaccca ttagcacccg catcgccttc
      241 tggataagct gaataccttc cagcggcatg taggttgtgt cgttacccgc cagtaatatc
      301 agcatggcgc ttgccgtaca gatgaccagg gtgtcgataa aaatgccaat catctggaca
      361 atcccttgcg ctgccggatg cggaggccag gacgccgctg ccgctgccgc gtttggcgtc
      421 gaacccattc ccgcctcatt ggaaaacata ctgcgctgaa aaccgttagt aatcgcctgg
      481 cttaaggtat atcccgccgc gccgcctgcc gcttcctgcc agccaaaagc actctcaaaa
      541 atagaccaaa tgacgtgggg aagttgcccg atatttatta cgcaaatcac catgctggtc
      601 agcacccaga ttatcgccat caacgggaca aacccctgca tgagccgggc gacgccatga
      661 agaccgcgag tgattgccag cagagcaaag acagcgagaa taatgcctgt caccagcggg
      721 ggaaaatcaa aagaaaaact cagggctcgg gcaacggcgt tcgcctgaac tccgctgaaa
      781 attatgccgt aggcgatgag caaaaagacg gcgaacagaa cgcccatcca acgcatcccc
      841 agcccgcgcg ccatatacca tgccggtccg ccacgaaact gcccattgac gtcacgttct
      901 ttataaagtt gtgcaaggga acattcggca aacgaggtcg ccatgccgat aaacgcggca
      961 acccacatcc aaaagacggc tctaggtcca ccggtggtaa tagccagcgc aacgccggcc
     1021 aggttgccgc tacccacgcg cgccgcaaga ctggtacaca atgactgaaa tgaggttaaa
     1081 ccgcctggct gtggatgaat gctattttta agacttttgc caaactggcg gatgtagcga
     1141 aactgcacaa atccggtgcg aaaagtgaac caacaacctg cgccgaagag caggtaaatc
     1201 attaccgatc cccaaaggac gctgttaata aaggagaaaa aatctggcat gcatatccct
     1261 cttattgccg gtcgcgatga ctttcctgtg taaacgttac caattgttta agaagtatat
     1321 acgctacgag gtacttgata acttctgcgt agcatacatg aggttttgta taaaaatggc
     1381 gggcgatatc aacgcagtgt cagaaatccg aaacagtctc gcctggcgat aaccgtcttg
     1441 tcggcggttg cgctgacgtt gcgtcgtgat atcatcaggg cagaccggtt acatccccct
     1501 aacaagctgt ttaaagagaa atactatcat gacggacaaa ttgacctccc ttcgtcagta
     1561 caccaccgta gtggccgaca ctggggacat cgcggcaatg aagctgtatc aaccgcagga
     1621 tgccacaacc aacccttctc tcattcttaa cgcagcgcag attccggaat accgtaagtt
     1681 gattgatgat gctgtcgcct gggcgaaaca gcagagcaac gatcgcgcgc agcagatcgt
     1741 ggacgcgacc gacaaactgg cagtaaatat tggtctggaa atcctgaaac tggttccggg
     1801 tcgtatctca actgaagttg atgcgcgtct ttcctatgac accgaagcgt caattgcgaa
     1861 agcaaaacgc ctgatcaaac tctacaacga tgctggtatt agcaacgatc gtattctgat
     1921 caaactggct tctacctggc agggtatccg tgctgcagaa cagctggaaa aagaaggcat
     1981 caactgtaac ctgaccctgc tgttctcctt cgctcaggct cgtgcttgtg cggaagcagg
     2041 cgtgttcctg atctcgccat ttgttggccg tattcttgac tggtacaaag cgaatactga
     2101 taagaaagag tacgctccgg cagaagatcc gggcgtggtt tctgtatctg aaatctacca
     2161 gtactacaaa gagcacggtt atgaaaccgt ggttatgggc gcaagcttcc gtaacatcgg
     2221 cgaaattctg gaactggcag gctgcgaccg tcttaccatc gcacctacac tgctgaaaga
     2281 gctggctgag agcgaagggg ctatcgaacg taaactgtct tacaccggcg aagtgaaagc
     2341 gcgtccggcg cgtatcactg agtccgagtt cctgtggcag cacaaccagg atccaatggc
     2401 agtagataaa ctggcggaag gtatccgtaa gtttgctatt gaccaggaaa aactggaaaa
     2461 aatgatcggc gatctgctgt aatcattctt agcgtgaccg ggaagtcggt cacgctacct
     2521 cttctgaagc ctgtctgtca ctcccttcgc agtgtatcat tctgtttaac gagactgttt
     2581 aaacggaaaa atcttgatga atactttacg tattggctta gtttccatct ctgatcgcgc
     2641 atccagcggc gtttatcagg ataaaggcat ccctgcgctg gaagaatggc tgacatcggt
     2701 gctaaccacg ccgtttgaac tggaaacccg cttaatcccc gatgagcagg cgatcatcga
     2761 gcaaacgttg tgtgagctag tggatgaaat gagttgccat ctggtgctca ccacgggcgg
     2821 aactggcccg gcgcgtcgtg acgtaacgcc cgatgcgacg ctggcagtag cggaccgcga
     2881 gatgcctggc tttggtgaac agatgcgcca gataagcctg cattttgtac caactgcgat
     2941 cctttcgcgt caggttggtg tgattcgcaa acaggcgctg atccttaact tacccggtca
     3001 gccgaagtct attaaagaga cgctggaagg tgtgaaggac gctgcgggta acgttgtggt
     3061 acacggtatt tttgccagcg taccgtactg cattcagttg ctggaagggc catacgttga
     3121 aacggcacca gaagtggttg ctgcattcag accgaagagt gcaagacgcg acgttagcga
     3181 ataaaaaaat ccccccgagc ggggggatct caaaacaatt agtgggattc accaatcggc
     3241 agaacggtgc gaccaaactg ctcgttcagt acttcaccca tcgccagata gattgcactg
     3301 gcaccacaga tcagaccaat ccagccggca aagtggatga ttgcggcgtt accggcaatg
     3361 ttaccgatcg ccagcagggc aaacagcaca gtcaggctaa agaaaacgaa ttgcagaacg
     3421 cgtgcgcctt tcagcgtgcc gaagaacata aacagcgtaa atacgcccca cagacccagg
     3481 tagacaccaa ggaactgtgc atttggcgca tcggtcagac ccagtttcgg catcagcaga
     3541 atcgcaacca gcgtcagcca gaaagaaccg taagaggtga atgcggttaa accgaaagtg
     3601 ttgccttttt tgtactccag cagaccagca aaaatttgcg cgatgccgct gtagaaaatg
     3661 cccatggcaa gaataatacc gtccagagcg aaataaccca cgttgtgcag gttaagcaga
     3721 atggtggtca tgccgaagcc catcaggccc agcggtgccg gattagccaa cttagtgttg
     3781 cccataattc ctcaaaaatc atcatcgaat gaatggtgaa ataatttccc tgaataactg
     3841 tagtgttttc agggcgcggc ataataatca gccagtgggg cagtgtctac gatcttttga
     3901 ggggaaaatg aaaattttcc ccggtttccg gtatcagacc tgagtggcgc taaccatccg
     3961 gcgcaggcag gcgatttgca gtacggctgg aatcgtcacg cgataggcgc tgccgctgac
     4021 cgctttaacc ccatttagtg ccgcacctac agggcctccc agccccgcgc cgcgcagcaa
     4081 accatgccca agtacgctca ttgctgcgtg ggtgcgtaaa atgcgggtca gttggctgga
     4141 aagcaaatgc gacacgcctt ttgccaataa tttgtctttc atcagcagcg gcagcagctc
     4201 ttccagctca ttcaccctcg catcgaacgc gtgcagaaac tcctgcttat gttcctcgtc
     4261 cattttcttc caggtattac gcagaaattg ttccagtaac tgttgctcaa tttcaaacgt
     4321 agacatctct ttgtcggctt tcagcttcaa tcgttttgaa acatcgagca aaatggcccg
     4381 atacaattta ccgtgtccgc gcagtttgtt ggcgatacta tcgccaccaa aatgctgtaa
     4441 ttctccagca atcagctgcc agttgcggcg atgttgctcg ggatgccctt ccatcgattt
     4501 aaacagttcg ttgcgcatca gtacgctgga gaggcgagtt ttgccttttt cattatgggt
     4561 gagcaatcgg gcgaaatttg ccaactgttc ctcactacaa tgctgaagaa aatccagatc
     4621 tgaatcattc aggtaattaa cattcatttt ttgtggcttc tatattctgg cgttagtcgt
     4681 cgccgataat tttcagcgtg gccatatccg atgagttcac cgtatgaccg gaaaaggtga
     4741 tttttgagac gcagcgttta ttgtcgttat cactgttaat gttgatccag tcagtggttt
     4801 gcccttcttt tatttctgaa ggaatattca gactctgact ggcgctacgg gc
//
LOCUS       DAJTMC010000225         4732 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.225, whole genome shotgun sequence.
ACCESSION   DAJTMC010000225 DAJTMC010000000
VERSION     DAJTMC010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4732
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.225"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(<1..804)
                     /locus_tag="OO130_003897"
     CDS             complement(<1..804)
                     /locus_tag="OO130_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCR5367112.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSGGLGGSNLTTPEWFNSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIPSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADP"
     gene            complement(797..3076)
                     /locus_tag="OO130_003898"
     CDS             complement(797..3076)
                     /locus_tag="OO130_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5367113.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIEGPADFNDRV
                     DELFLAHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQAARRIQAQTYFIDL
                     PCWAQSEEVDDSPDTQEESQALLLRATRMDNSDTLWDHLFEDESQQIALPSALAHYFA
                     QLRGDFPGDALNRQREAFMARWIAWAVQRNNGDVLVVCGGWHAPALAKMWRECPQEIN
                     KPELPSLANAVTGCYLTPYSEKHLDVLAGYLSGMPAPVWQNWCWQWGLQQAGEQLLKT
                     VLTRLRQHNLPASIADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTAISLPAE
                     LTLNRFTPDGLAQSQVLHRLAILEIPGIVRQQGSTLTLAGNGEEHWKLTRPLSQHAAL
                     IEAACFGATLQEAARHKLEADMLDAGGIGSITTCLSQAALAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVNLSGVSLSAAVALLERRSQAIHAPALDRGAALGAL
                     MRLEHPNASAEAALTMLAQLSPAQSGEALHGLLALARHQLACQPAFIAGFSSHLNQLS
                     DANFINALPDLRAVMAWLPPRERGTLAHQVLEHYQLAQLPVSALQMPLHCPPQAIAHH
                     QQLEQQALASLQHWGVFHV"
     gene            complement(3087..4175)
                     /locus_tag="OO130_003899"
     CDS             complement(3087..4175)
                     /locus_tag="OO130_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HCR5367114.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATAISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
BASE COUNT         1103 a         1254 c         1295 g         1080 t
ORIGIN      
        1 cggatcggca acgtctgccg tgagatcaac gacgcttgtg tcaaacgcca ccagatgggt
       61 acgaatgccg ggtaactgcc acaaacaggc cgccatcacc gcagagggga tcaccgaatc
      121 gaccatcgag ccgctttgat caaccagtaa gaccagttgc cattgttcgc tttggcgttt
      181 aatgcggctg ttaaagcggg gggattcgat atacaacttg ccgtgttgcg ggtgccagtg
      241 ttgcaggttg gcgcgcagag tacttttgaa atcaaagttt cgcgccagtg gaataaatga
      301 gcggcgacgg cgatcgcgga caccagaaaa agcctgacga acttcctttg ccagtcgcgc
      361 cataatttct tcaacaacct ggcgcacaat cctgcgggcg gcagccagta cttcggggtt
      421 catcagatgt ttggtgtgca aaacggcgcg tagcaggctt tcggaaggct gcatacgttc
      481 cagcacgtcg agatttgtca ccacatcttc aatgccgtag cgcagtacgg catcgctttc
      541 cagccgctca atcacctgtt gcggaaacag cgtgtggata ctgttgaacc actcaggagt
      601 ggtgagattt gagccaccta atccaccaga gcgttcacca cgctggagcc gttcaggatc
      661 gcgcccatac agccactcca gcgcgtgatc tatctgccgg gcgttgtcat ccagcccaca
      721 aagcgtcgtt tctgccgctt cgccaagaat taatcgccag cgttgtagct cacgggtggt
      781 cagaagatcg ttcagttcag acatggaaaa ctccccagtg ttgcagcgat gccagtgcct
      841 gctgttcgag ttgttgatga tgtgcaatgg cttgcggtgg acaatgcaac ggcatttgca
      901 gtgccgaaac gggaagttgc gccagttgat aatgctcaag cacctgatgc gccagcgtcc
      961 cgcgttctcg tggcggtagc caggccatca ccgcgcgtaa atcgggcagg gcattgataa
     1021 aattggcatc actcagttga ttcaaatggc tgctgaaacc ggcgataaat gcaggttgac
     1081 aggccagttg atgacgggct aatgccagca aaccgtgcag cgcctcgccg gattgtgccg
     1141 gggataactg cgccagcatc gtcagcgccg cttcggcact ggcgttggga tgttccagac
     1201 gcattagtgc gccaagagcc gcgccgcgat ccagcgccgg ggcatgaatc gcctgactgc
     1261 gtcgctccag taaggctacc gccgcagaaa gcgaaacgcc ggataaatta acgccgctat
     1321 gtagatcgcg cagaatatgg caaagcgctt gccagctatg caggtgagcg tgaaactcct
     1381 tttcatccgg tctgccgtta gattcacaca gccacagcgt gcgatcgata gccgcgcata
     1441 acgtcgtttg taatatctgc gcgccttgca taccgctaat ttcatccagc cgccataagg
     1501 catatagcac ttccagcgcc tggcccattt cggcaaattg attttcctgg gcgattaata
     1561 atgtgagttg ctccagtaat tgttgactga aggacgccag acccgctaac gccgcctggc
     1621 taagacatgt ggtgatactg ccgatgccac ccgcgtccag catatcggct tctaatttat
     1681 ggcgtgcggc ttcctgaagt gttgcaccaa agcatgcggc ctcaatcaat gccgcatgct
     1741 ggctaagcgg gcgggttaat ttccagtgtt cttcaccgtt gcctgcaagt gtcagtgtac
     1801 ttccctgctg gcgtacaatc ccagggatct ccagtattgc cagccgatgt aacacctgac
     1861 tttgcgccag cccatcgggg gtaaagcgat ttaaggtaag ctccgccgga agagagattg
     1921 cggtacgttc cagttcgcag gtgacatctt ttggcagagg cggttgtggc gtggaagggg
     1981 caagtttacc gaatccgtca cccgctaatg tgtctatcaa cgttagcaga atcggatcgg
     2041 tatcgggatg aataacgccg cgatagctcc acggcaacgg tgcattcagg gcttctttaa
     2101 tcagcgagcc tgctatggca tccagccagt cagtgcgtaa cggtagtgta tgaccgcgca
     2161 actgtgccag tgccattgca tgcagatgtg cggcagccat atccgcgatc gaagcaggca
     2221 agttgtgctg gcgcaaacgg gtgagaaccg tttttagcag ttgttcaccg gcctgctgta
     2281 agccccactg ccagcaccag ttttgccaga ccggggcagg cataccggaa aggtatcctg
     2341 ccagcacatc aaggtgcttt tcactgtagg gggtgagata acaacctgta acggcatttg
     2401 ccagcgaggg caattctggc ttgttaattt cctgcggaca ttcgcgccac atttttgcca
     2461 gtgcaggagc gtgccagcca ccgcagacga ctaacacgtc gccattattt cgctgcaccg
     2521 cccatgcaat ccagcgagcc ataaaggctt cgcgctgacg attgagtgca tcgccgggga
     2581 aatcgccccg cagttgggca aaatagtgcg ccagcgcaga gggtaatgca atttgctggc
     2641 tttcatcttc gaacaagtga tcccacaggg tgtcgctgtt atccatgcgg gtggcacgca
     2701 gcagcaaggc ctggctttcc tcttgcgtat caggcgaatc gtccacttct tcactctgcg
     2761 cccagcaagg caaatcgatg aagtaagttt gtgcctgaat acgacgtgcg gcttgtagcg
     2821 cctgccactc cggcgaaaat tcagcaaatg gcgtccaggc accacgcccg ggggctgcac
     2881 cgtcctgata ctggcaataa ctgtaaatag ctaccggaag ctggtgggct aaaaacagtt
     2941 cgtctacccg gtcattaaaa tcagccgggc cttcaatcaa tacgtatcgt ggccgctggc
     3001 tttcgattaa cgatttcacc agccgggcgc aggccggact atgatgccgg atgccgacaa
     3061 ttaacggctc gctcatgcat ctttcctcac ggcaggcggt ggcgggcttg ataataagcc
     3121 tgccagtgag cttctttatg tgtggcgact cgttgttcga agtaacggcg cagacgagcg
     3181 cgatcttctt cgttatcttt gacgatggtt ccggcaatgc agtccaccag gtcggctggc
     3241 tcacctgcgc gattcgctaa aaaccaggcg cggacgccta cggcgtgggc cacgttcacg
     3301 gcttcagcgg tggacataat cgctgttagt gtatccattg acgttttctt ttcgccgttg
     3361 gcacgcagat cgcggaaggt gcggaccagt aactccagta ccgcgtcagg cactttatgc
     3421 ggaataccgc tatgagccag caaacgcgcc gaagcactgg cgaccagttc cagctcctgg
     3481 gcgaaatcca taatcggaaa gacagtttca aaatcgaagc gacgctttag cgcagcactc
     3541 atttcattga cgccgcgatc gcgggtattg gcagtggcaa taatattaaa gccttctcgc
     3601 gcatagagct gactggcttc accagtgagt tccggcaccg tcatcaccct gtcagaaagc
     3661 atacctaaca gacagtcttg cacttccagc ggtgtacgcg ttatctcttc aaaacggacg
     3721 attttgccgt cgcgcattcc ctggtaaagc ggggcaggga ccagcgcttc cgttgatgga
     3781 ccatgattga taagcagggc gtagttccag ccgtacttga tttgatcttc ggtagtagat
     3841 gcgccgccct ggatggttaa cccggcatcg ccgctaatag cggttgccag taattcagac
     3901 aggagagatt ttgcggtgcc aggttccccc accaacatca aaccgcgtcc ggtggcaaga
     3961 gtgactaaca tacgttcgac agcggaggga ctaatcacca ctttacggct aatattttgc
     4021 gcgctatcgc caaggataaa agcacgggcc gcaggcaaac ttaactgcca accgggcggg
     4081 caaggtgcgt tatcattttg ttttaatttt gccagttcat cggcgtataa cacagcagca
     4141 ggcggacgct gcagatgatt attctgcggt gacatatatt catccctgaa atggattaat
     4201 gaaataaacg atattttacg cgcaataaat gtttggcgca ttagttgttt gagcgaaaaa
     4261 tatctggaaa gcagatcaca gtaaataaaa aaacctgctg gtgaggcagg ttcttttatt
     4321 tactactttc aggcctaaca ccacagcctg tgcaacatcg tgttccaacg aacgaattat
     4381 aaccacggct ggaccccgtt gcaattaaga taatgagatt tataatccag accgttggga
     4441 cgttgcgccg gatcgaatct atttcaagtc ctggctaccg gacgggtggt gctgcaggtg
     4501 gtgccctgat ccggagtgca acatcgtggc ccaacatacg taaaccagta tcgacaggca
     4561 gtgtaagggt aaggtgctgg tgttgcgctc cttaataagg agagcggatg gacacgctga
     4621 cacaaaagtt aaccgtgctc attgctgtac tggagctatt agtggctctg ttacggttga
     4681 ttgatttgtt gaagtaacgg gcaacttgaa acgcatctaa gggggaatac ag
//
LOCUS       DAJTMC010000226         4673 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.226, whole genome shotgun sequence.
ACCESSION   DAJTMC010000226 DAJTMC010000000
VERSION     DAJTMC010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4673
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.226"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            60..1859
                     /gene="cysJ"
                     /locus_tag="OO130_003900"
     CDS             60..1859
                     /gene="cysJ"
                     /locus_tag="OO130_003900"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HCR5367115.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     PETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKD
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDIEAADEFLSELRVERRYQRDVY"
     gene            1859..3571
                     /gene="cysI"
                     /locus_tag="OO130_003901"
     CDS             1859..3571
                     /gene="cysI"
                     /locus_tag="OO130_003901"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HCR5367116.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPHHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            3646..4380
                     /gene="cysH"
                     /locus_tag="OO130_003902"
     CDS             3646..4380
                     /gene="cysH"
                     /locus_tag="OO130_003902"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HCR5367117.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIRPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMSEEETRFFGLKRECGLHEG"
BASE COUNT         1153 a         1218 c         1334 g          968 t
ORIGIN      
        1 tgttttatgg gcgctgacag ggcgcagaaa cagctttgct tactggaaca taacgacgca
       61 tgacgacaca ggtcccacct tccgcgttgc ttccgttgaa cccggagcaa ctggcacgcc
      121 ttcaggcggc cacgaccgat ttaacgccta cccagcttgc ctgggtttct ggctatttct
      181 ggggcgtact caatcagcag cctgctgcgc ttgcagcgac gccagcgcca gccgcagaaa
      241 tgccgggtat aactattatc tccgcctcgc aaaccggcaa tgcgcgccgg gttgctgaag
      301 cattacgcga tgatttatta gcagcaaaac tgaacgttaa gctggtgaac gcgggcgact
      361 ataaattcaa acaaatcgcc agcgaaaaac tgctcatcgt agtgacgtca acgcaagggg
      421 aaggggaacc gccggaagaa gccgtcgcgc tgcataagtt cctgttctcc aaaaaagcgc
      481 caaagctgga aaacaccgcg tttgccgtgt ttagcctcgg cgatagctct tatgaatttt
      541 tctgccagtc cgggaaagat ttcgacagca agctggcgga actgggtggt gaacgcctgc
      601 tcgaccgtgt cgatgccgat gttgaatacc aggctgctgc cagcgagtgg cgcgcccgcg
      661 tggttgatgc gcttaaatcg cgtgcgcctg tcgcggcacc ttcgcaatcc gtcgctactg
      721 gcgcggtaaa tgaaatccac accagcccgt acagcaaaga cgcgccgctg gtggctagcc
      781 tctctgttaa ccagaaaatt accgggcgta actctgaaaa agacgttcgc catatcgaaa
      841 ttgacttagg tgactcgggc ctgcgttacc agccgggtga cgcgctgggc gtctggtatc
      901 agaacgatcc ggcactggtg aaagaacttg tcgaactgct gtggctgaaa ggcgatgaac
      961 ctgtcaccgt cgagggcaaa acgttgcctc tgaacgaagc gctacagtgg cacttcgaac
     1021 tgaccgtcaa caccgccaac attgttgaga attacgccac gcttacccgc agcgaaacac
     1081 tgctgccgct ggtgggcgat aaagcgaagt tacagcatta cgccgcgacg acgccgattg
     1141 tcgacatggt gcgtttctct ccggcgcaac tggatgccga agcgctgatt aatctgctgc
     1201 gcccgctgac cccgcgcctg tattccatcg cctcctcgca ggcggaagtc gagaacgaag
     1261 tacacgtcac cgttggtgtg gtgcgttacg acgtggaagg ccgagcccgt gccggtggtg
     1321 cctccagctt cctcgcggat cgcgtggaag aagagggcga agtccgcgta tttatcgaac
     1381 ataacgataa ctttcgcctg cccgctaacc cggaaacccc ggtgattatg attggcccag
     1441 gcaccggtat tgcgccgttc cgcgccttta tgcagcaacg cgccgccgac gaagcgccag
     1501 gtaaaaactg gctgttcttt ggtaatccgc actttacgga agacttcctg taccaggtgg
     1561 agtggcagcg ctacgtcaaa gatggcgtgc tgacacgtat cgatcttgcc tggtcgcgcg
     1621 accaaaaaga aaaagtttac gtacaagaca aactgcgcga acagggcgcg gagctgtggc
     1681 gctggatcaa tgatggtgcc cacatttatg tctgcggcga cgctaatcgc atggcgaaag
     1741 acgttgagca ggcacttctg gaagtgattg ccgaatttgg tggcatggac atcgaagcgg
     1801 cggatgaatt tttaagtgag ctgcgcgtag agcgccgtta tcagcgagat gtctactaat
     1861 gagcgaaaaa catccagggc ctttagtggt cgaaggaaaa ctgacagacg ccgagcgcat
     1921 gaagcttgaa agcaactacc tgcgcggcac cattgcggaa gatttaaacg acggtctgac
     1981 cggcggcttt aagggcgaca acttcctgct gatccgcttc cacggcatgt atcagcagga
     2041 tgaccgcgac atccgcgccg aacgtgctga acagaagctg gagccgcacc acgcgatgct
     2101 gcttcgctgt cgtctgccgg gtggggtgat taccactaaa cagtggcagg cgatcgacaa
     2161 atttgccggt gaaaacacca tctatggcag cattcgcctg accaaccgcc agacattcca
     2221 gttccacggc attctgaaaa agaacgtcaa accggtgcac cagatgctgc actcggtcgg
     2281 tcttgatgcg ctggcgacag ctaacgacat gaaccgtaac gtactctgca cctcgaaccc
     2341 ttacgagtcg cagctgcacg cggaagcgta cgagtgggcg aagaagattt ctgagcatct
     2401 gttgcctcgt acccgcgcgt atgcggagat ctggctcgac caggaaaaag tcgccactac
     2461 tgatgaagaa ccgatccttg gtcagaccta cctgccgcgt aagttcaaaa ccacggtagt
     2521 gatcccgccg cagaacgata tcgatctgca tgccaacgac atgaacttcg tggcaatcgc
     2581 cgaaaacggc aagctggtag gcttcaacct gctggtgggc ggtgggcttt ctatcgaaca
     2641 cggcaacaag aaaacctacg cccgcacggc gagcgagttt ggctatctgc cgctggagca
     2701 tacgctggcg gtggccgaag ctgtcgtgac aactcagcgt gactggggta accgaaccga
     2761 tcgtaaaaat gccaaaacca aatacacgct ggagcgcgtg ggggttgaga cgtttaaagc
     2821 ggaagtggag cgtcgcgcgg ggatcaaatt tgaaccgatc cgtccatatg agttcaccgg
     2881 acgaggcgat cgtattggct gggttaaggg cattgatgat aactggcacc tgacgctgtt
     2941 tatcgaaaat ggtcgcatcc ttgattatcc ggggcgtccg ctgaaaaccg gcctgctgga
     3001 gatcgcgaag atccacaaag gcgatttccg cattacggcg aaccagaatc tgatcatcgc
     3061 cggtgtaccg gaaagcgaga aagcgaagat cgagaagatc gccaaagaga gcgggttaat
     3121 gaatgccgtc acgccgcagc gtgaaaactc gatggcctgc gtgtcattcc cgacttgccc
     3181 gctggcgatg gcggaagcag agcgtttcct gccgtctttt atcgacaaca tcgataattt
     3241 aatggcgaaa catggtgtca gcgatgagca tatcgtgatg cgtgtaacag gctgcccgaa
     3301 cggttgtggt cgcgcgatgc tggcggaagt gggcctggtg ggtaaagcgc cgggtcgcta
     3361 caacctgcat cttggcggca accgcattgg gacacgtatc ccacggatgt ataaagaaaa
     3421 catcaccgag ccggaaatcc tggcgtcgct tgatgaactg atagggcgct gggcgaaaga
     3481 gcgcgaagcg ggtgaaggct tcggcgactt tacggtgcgt gcgggcatca ttcgcccggt
     3541 gctcgatccg gcgcgcgatt tgtgggatta accatcagcc cggtcttgta ggcctgataa
     3601 gaacgcgtga gcgtcgcatc aggcaaggca aacagtgagg aatctatgtc caaactcgat
     3661 ctaaacgccc tgaacgaact gccgaaggta gatcgcattc tggcgctggc ggaaactaac
     3721 gccgaactgg aaaaactgga cgctgaaggc cgcgtagcct gggcgctgga taatctgccc
     3781 ggtgaatatg tactttcttc cagctttggc attcaggcgg cggtgagcct gcatctggtg
     3841 aatcaaattc gcccggatat tccggtgatc ctcaccgata cgggttactt gttcccggaa
     3901 acctaccgct ttattgacga gttaacggac aaactcaagc tcaacctgaa agtgtaccgt
     3961 gctaccgaaa gcgcagcctg gcaggaagca cgctacggaa aactgtggga gcagggcgtt
     4021 gaaggcattg aaaagtacaa tgacatcaac aaagtcgaac cgatgaaccg ggctctgaaa
     4081 gaacttaacg cgcaaacctg gtttgctggc ctgcgccgtg aacaatccgg cagtcgcgcc
     4141 aatttaccgg tgctggcaat tcagcgtggc gtatttaaag tgctgccgat tatcgactgg
     4201 gataaccgaa ctatttatca gtacctgcaa aaacatggcc tgaaatatca cccattatgg
     4261 gatgaaggat atttatcggt cggtgatacc catacaaccc gtaaatggga acctggcatg
     4321 tcggaagaag aaacacgttt ctttggctta aaaagggaat gtggattgca cgaagggtaa
     4381 atattgccgg ataaattaaa cgcctctgtc agaaatgatg ggggcgtttt tattttgcga
     4441 gcagtattcc tgattttaaa cgtgaacgcc gctggaagaa ttatcaggta tagaatggcg
     4501 tcgggtgctt gaggctgtct ggcgttaagc attcgcgagg ttccagatgg acaaaagccc
     4561 caggcgatat ttctatcaac ctgaggccag cgttcgaacc caaacaattc gaatgttagt
     4621 ctcttctttg cagggaggca agacatgctg acaaaatatg cccttgtggc aat
//
LOCUS       DAJTMC010000227         4662 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.227, whole genome shotgun sequence.
ACCESSION   DAJTMC010000227 DAJTMC010000000
VERSION     DAJTMC010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4662
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.227"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(<1..755)
                     /locus_tag="OO130_003903"
     CDS             complement(<1..755)
                     /locus_tag="OO130_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CSS-motif domain-containing protein"
                     /protein_id="HCR5367118.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVMEMINIDLLTAMLLEPQLQQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQRFPFTINVNGPGATALAWHYLPTQLPLAVLL"
     gene            complement(886..2250)
                     /gene="sdaA"
                     /locus_tag="OO130_003904"
     CDS             complement(886..2250)
                     /gene="sdaA"
                     /locus_tag="OO130_003904"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCR5367119.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            complement(2434..3012)
                     /locus_tag="OO130_003905"
     CDS             complement(2434..3012)
                     /locus_tag="OO130_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HCR5367120.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVIGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            complement(3016..4377)
                     /gene="pabB"
                     /locus_tag="OO130_003906"
     CDS             complement(3016..4377)
                     /gene="pabB"
                     /locus_tag="OO130_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HCR5367121.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVAEPICTLTTFGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVTKILRQ
                     LEK"
     gene            4451..4630
                     /locus_tag="OO130_003907"
     CDS             4451..4630
                     /locus_tag="OO130_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HCR5367122.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
BASE COUNT         1171 a         1199 c         1211 g         1081 t
ORIGIN      
        1 cttagcagca ccgccagcgg taattgtgtt ggaagatagt gccatgccag cgccgtagcc
       61 cccggaccat taacgttaat ggtaaaagga aagcgttgcg aagaaacctg atagttttca
      121 ttgtcttcag gttgcggaag ggaatctacc agcccattac catagagcaa atgccgtttg
      181 tccaccgtca ggctggcgct actgatttgc tgcagttgtg gctcaaggag cattgccgtc
      241 agtaagtcga tgttaatcat ctccatgacc ccagcattgc tactgcccgc tgcaggcgtc
      301 cattgaatca aaaccggact gcctttaatc agggcacggt cgatcgtcag gcgtaaaagt
      361 ggttgcggtg caggaagttc agccagaatg tccacgacgg gcacattgcg ataaccaaaa
      421 atgctggagc aatagagtgt gccgtcttgc accaggccaa tggatcgcac agtttggagt
      481 tttgccgcct gtttacgtaa tggcaaatgg gcgacagagc agggcagacc aatcagcgga
      541 agcaagactt cgctacctgc ctgtagggga agcagtactt tatctaattc ctctacagcg
      601 tgattggcga attgtactac ccgttgttga tttaagttac gctgtgaaat aaatcgcacg
      661 ctcagggtcg aagcgagcgt aacgagtgcg caaatcgtac aaacaatcat tcgattacgt
      721 cgataggttt taatgatccg ttgtgctttt tgcatgcggg ccaccctgta agcgtatggc
      781 gacaaacgcc tgacgcaaca gtggatgtgt agtggggaat attacagcag acgagaaagc
      841 gggtataaat tcgcccatcc gttgcagatg ggcgagtaag aagtattagt cacactggac
      901 tttgattgcc agaccaccgc gtgaggtttc gcggtatttg gcgttcatgt ccttaccggt
      961 ttcgtacatc gtttcgatga ccttatccag cgagacgcgc ggtgcactgg tgcggcgcag
     1021 agccatccgt gcggcgttaa tcgccttcac agaggcaatg gcattacgct caatgcacgg
     1081 cacctgaacc tgccctgcaa ccgggtcgca ggttaaacca aggttgtgtt ccatgccaat
     1141 ttccgccgcc acgcaaacct gttccgggct accgcccagc agttctgcaa gacccgcagc
     1201 agccattgaa caggcaacac ccacttcgcc ctggcaacca acttccgcac cggaaataga
     1261 ggcgttcatt ttatacaatg caccaatcgc gcccgctgcc ataaagtaac gggtatagat
     1321 gtccgggctg accgattcaa taaagtggtc atagtaagcc agcactgccg gaacgatacc
     1381 gcaggcaccg ttggttggcg cagttaccac acgaccaccg gcggcgtttt cttcgttaac
     1441 tgccagcgca aacatgttta cccagtcaat gacattcatc ggatcgttag acagtttatc
     1501 gctggaaacc agcatccggc gcagggcaga cgcacgacgt ggcacgcgca gcgggcctgg
     1561 cagtacacct tcggtgttca tcccgcgatc gatacatgcc tgcatggttt gccagacatg
     1621 cgcgaaatac tcgtcgatct ctttcttgct gtgcagcgcc agttcgttct gcatagcgag
     1681 accagacagc gaatagccgg tttcattaca gtacgcgagc agttcggtgg cagatttgaa
     1741 cggatacggc acgctcactt cgttggcagc atcctgacca aagtgttctt catcgacgat
     1801 aaaaccgccg ccgatggaat aataagtttt gctgtagacg acttcatcgc cgttataggc
     1861 gtggatttgc ataccgtttt catgcagcgg caggttgccg ttatgaaaac gcatcccgtt
     1921 gtcgcgcggg aaatccactt catgccgtcc ctgtgccagc agcagacgtt cgcgctcttc
     1981 tacgtcgcga ataaaaccgg gaatactgtc gatatccacg gtggcaggtt cgttacctgc
     2041 aagacccata ataatggcaa tatcggtgtg gtggccttta cccgtcagcg acagtgaacc
     2101 ataaacgtcc acggcaacgc gagtaacgct atccagtaag cctttttcga ccagatcatc
     2161 gacgaactgt ttacccgcct tcataggccc tacggtatgg gaagatgagg gaccaatccc
     2221 caccttaaac atgtcgaata gactaatcac gataatactc ctgacaaggt gactggactt
     2281 ccagtaacga actaataaca gcgcatagtg taagagggaa cgcggcgact ggcttaacta
     2341 ttcacatgaa ttaaactaat caaaaaccgc gatttaacta atattgcgat gattgtctca
     2401 agtagtgttt tgtagatgta aagataagta tagtcagggt ttcacaccaa tttgcagcgc
     2461 cagctcacga attatgcctg cggtcattcc ccatacaaaa tactgttcgt accaggacag
     2521 ccatacccga tgtgaatcgc cacggcggta gatatctaaa gggtgataac gacccagatg
     2581 taatgcctgg gcgagcggca tttcaaacac cgccgagact tcatcttcac tggcgcgata
     2641 cggcagatcg ggcgggataa tgccgatcac tggggttacc tgatagccag ttacgctatc
     2701 gacgggcggc agcacgccga taacttcaac ggcggaaggc ggtatagcga cctcttcttc
     2761 agcttcgcgc agcgcggcgg cgataactga tgcgtccgtg tcatcgactg cacctccagg
     2821 gaatgccact tgtccagcgt gtttacgcag atgaattgaa cgctgagtca gcaacaaccc
     2881 cggttgcggt cgacggacga tggggattaa cacggcagcc tgacgatgat ttagggtttc
     2941 ccggttaatt tgcgggcgca aaagttgaaa gcgagataaa aaatcatcaa gcgtcaggct
     3001 acggtattcc acgtcttact tctccagttg gcgtaatatc ttagtaactt tatcaaaagt
     3061 ttcctgatat tccgcttctt cctggctatc ggcgacaatt ccaccgcccg cagagcagta
     3121 tatttgtccg ttaatggcag tcagcgtgcg gatagtaatg ctggtatcca tgttgccgca
     3181 aaagctcaaa tagccaatgc tgccgcacca ggcattacgt cgctgcggtt ccagttcgtc
     3241 gataatttcc atagcccgta ctttcggagc cccggttatt gagccaccag gaaaagctgc
     3301 gcgcagcaga tcgctggcgt gtaactgttc tggtagtcgc gccgttatag tgctgaccag
     3361 atgatgcacg gcagggaagg gttccaccac gaagagctct ggtactttta ccgaaccggc
     3421 tacggcaaca cgaccgatat cattacgcat taaatcgaca atcatcagat tttcggcacg
     3481 atctttcgct gagttcgcca gtttttctgc ttgtttgcta tcttcctgag gatcgggcag
     3541 gcgtggtagc gtgcctttaa tcgggcgggt ctggatttca ctattatcac aaagaataaa
     3601 ccgctctggc gaaaggctta aaattgcacc ctgttcaaga cgtaaaaaag cgctaaatgg
     3661 cgcgcggttg gcctgattaa gctgaaggaa tgcctgccat tcatcgccag aataggtcgc
     3721 atgaaaacgc tgggcgagat tcacctgata gcaatcaccg ctgtgcagat attcctgtac
     3781 ctggcgaaat ttttcgccgt actgctcgcg ggtcatattg gattgccagt cggaagtgag
     3841 cgtgaaatct tcctgcggcg agaattgctg gctttccagc caggcccgac gggcattgac
     3901 atcattatga ctcagcaaag aaactgtatg acgctggtgg tcgacaatga gcgcccaatc
     3961 gtagataccc actgccatat ccggcagaac gatatcttgt tccgcaattt ctggcagtga
     4021 ctcaaaacgg cggcccagat cgtagccaaa caaccccagt gcgccgccct gaaatggcaa
     4081 atcttcgtta tgcgttgggc gaatgtctgc gcgatccagc acctgctgga gcacctgtag
     4141 cgggtcatca gtggtcgttg tgcgtttttc gctttcacta acaacggttt ctttaccgaa
     4201 agtggttaaa gtgcaaatcg gctcggcgac cacaatatca aagcggctat acggatgatc
     4261 ggcatagccg gagtgtaaaa gcatcgccca cggcaggtgg cttaagcggg agaaataaaa
     4321 ttcagcggcg tcctgacgcc agggtaaagt aatcacagcg ggagataacg tcttcatcag
     4381 tcctgactct actggctatg tgccgggtag catgaaataa ttagcgccaa caatttagca
     4441 ggagttaaca atgtttgcag gtttaccttc actcacccat gaacagcagc aaaaagctgt
     4501 cgagcggatc caggaactga tggcacaagg gatgagcagc ggccaggcaa tcgcgctggt
     4561 ggcagaagag ctgcgtgcca accactccgg cgagcgaatc gtggcgcgtt ttgaggatga
     4621 agacgagtaa cacactaatc cgtatcgatt tcgttataga ag
//
LOCUS       DAJTMC010000228         4585 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.228, whole genome shotgun sequence.
ACCESSION   DAJTMC010000228 DAJTMC010000000
VERSION     DAJTMC010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4585
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.228"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            253..615
                     /gene="queD"
                     /locus_tag="OO130_003908"
     CDS             253..615
                     /gene="queD"
                     /locus_tag="OO130_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HCR5367123.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            693..1961
                     /locus_tag="OO130_003909"
     CDS             693..1961
                     /locus_tag="OO130_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR5367124.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGVTTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRPLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWQLVGDALRSCVNTGISVRGMGMALTGAQAAQTLISACQHREP
                     QNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGWYRAWPALMQDISRDLWDQGDKPV
                     PPLRQLFWRHLRRHGLWHLAGDVIRSLRCL"
     gene            1952..2212
                     /locus_tag="OO130_003910"
     CDS             1952..2212
                     /locus_tag="OO130_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HCR5367125.1"
                     /translation="MSVAHNLWRVADAPHIVPADSVERQMVQWLINACPAGLFSLTPE
                     GDLRVDYHGCLECGTCRLLCDESTLQQWRYPPSGFSITYRFG"
     gene            2229..2804
                     /locus_tag="OO130_003911"
     CDS             2229..2804
                     /locus_tag="OO130_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HCR5367126.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDACNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(2952..3812)
                     /locus_tag="OO130_003912"
     CDS             complement(2952..3812)
                     /locus_tag="OO130_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HCR5367127.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDALVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSVRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGIQQLNIVPGALPDWLVSTEDLKNV
                     TRDPLAEARRVLVVGQGGEADNQEIAMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQADVGVVDDWKVVLEA
                     LVTNIHADCQ"
     gene            complement(3809..>4585)
                     /locus_tag="OO130_003913"
     CDS             complement(3809..>4585)
                     /locus_tag="OO130_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HCR5367128.1"
                     /translation="NILLAFKAEPDAGMLAEKEWQAAAQGKSGPDISLLRSLLGADEQ
                     AAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPEF
                     VARHIAEWQHQNPLDLIITGSQSSEGQNGQTPFLLAEMLGWTCFTQVERFTLDALFIT
                     LEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPAM
                     QCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
BASE COUNT         1054 a         1234 c         1165 g         1132 t
ORIGIN      
        1 agataaggtt aacggggtga acggtgtgga ttaaagacgg gatagcgata actaataacc
       61 aaatcgacta acctgtttta gcaatagtct ttaacaacaa aatagattaa ccaacccaat
      121 gaaaaacaaa tgaatttagc caatcattaa gataaatcag cgattttgcg caacaaagtc
      181 gttttagata atgcgaaaaa acagcctttc cggtactcta cggcggtttt atcgtcctgt
      241 agagaaatta tgatgtccac cacgttattt aaagatttca ccttcgaagc cgctcaccgc
      301 ttaccacacg tcccggaagg gcataaatgt ggtcgcctgc acgggcattc ctttatggtg
      361 cgactggaaa ttaccgggga agtcgatccg catacgggct ggattatcga tttcgctgaa
      421 ctaaaagcgg cgtttaagcc aacttatgag cgcctcgatc accattatct caatgatatt
      481 ccaggtctgg aaaacccaac tagcgaagtt ttagcaaaat ggatttggga tcaggttaaa
      541 cccgttgtgc cgctgttaag tgcggtgatg gtaaaagaaa cctgcaccgc tggttgtatc
      601 tatcgcggcg aatgataaga gtgtgtcggc ggtcaatttc ccttaagtaa cgctatgtta
      661 gggtattgtg ttcaggatat ctggggcatg acatggaaga cgactgcgac attattatta
      721 ttggtgccgg tattgcaggc accgcttgcg cgttacgctg cgcgcgagcg ggtttatccg
      781 ttttgttact ggaacgcgct gaaatccccg gcagcaaaaa tctttccggc ggacggttat
      841 atacccatgc actcgcggaa ctcctccctc agtttcatct gaccgcgcct cttgaacgac
      901 gcatcactca cgaaagcctt tccctgttaa cgcccgatgg cgtaacgacg ttttccagct
      961 tacagcccgg cggtgaatcc tggagtgtat tacgtgcacg gttcgatccg tggctggttg
     1021 ccgaagccga aaaagaaggt gtcgaatgta tccccggagc gacggtggat gcactgtatg
     1081 aagaaaacgg cagagtctgt ggcgttattt gtggtgacga tattctccgc gcccgttatg
     1141 tggtgctggc agaaggtgcc aacagcgtcc tggctgaacg tcacgggtta gtgactcgtc
     1201 ctgctggcga agcgatggcg ttggggatca aagaagtgct gtcgctggaa acatccgcta
     1261 ttgaagaacg ttttcatctg gagaataacg aaggcgcagc gttgctgttc agcggcggaa
     1321 tctgtgatga cttacccggc ggcgcatttc tttatactaa tcaacaaacg ctctcgttag
     1381 ggattgtttg cccgctctct tcccttacgc aaagtcgtgt tccggcaagc gagctgctga
     1441 ctcgctttaa agcgcatccg gcagtgcgcc cgcttatcaa aaacacggaa tcactggagt
     1501 atggtgcgca tctggtgcca gaaggtggct tgcacagtat gccggtgcaa tacgccggta
     1561 acggctggca gctggtgggc gatgcgttgc gcagttgcgt caataccgga atttccgtgc
     1621 gcggcatggg tatggcgctg actggcgcgc aggcggcaca aacgctgata agcgcctgcc
     1681 agcaccgcga gccgcaaaat ctgtttgcgc tttatcatca caacgtagag cgcagcctgc
     1741 tatgggatgt tctacaacgt tatcagcatg ttccggcgct tttgcaacgc cctggctggt
     1801 atcgggcgtg gcctgcgtta atgcaggata tttcccgcga tttatgggat cagggtgata
     1861 aacctgttcc accgctgcgc cagttattct ggcgtcattt acgtcgtcat ggcctgtggc
     1921 atctggcggg cgatgttatc aggagtctgc gatgtctgta gcccataatc tctggcgcgt
     1981 tgctgatgcg ccgcacattg ttccggctga ctccgttgag cgtcagatgg tacaatggtt
     2041 gattaacgcc tgtccggcag gtcttttttc gctcacaccg gaaggtgact tacgcgttga
     2101 ttatcacggc tgcctggagt gtggcacctg ccgtttgctg tgcgacgaat ccacactaca
     2161 acagtggcgc tatccgcctt ccggattcag catcacctac cgctttggat aaataataag
     2221 gataatttat gcccctctta cacctgctcc gccagaatcc ggtgattgct gccgttaaag
     2281 ataatgccag cctgcaactg gcaatcgact ctgaatgcca gtttatttcc gtgttgtacg
     2341 gcaatatctg caccatcagt aatatcgtta aaaagattaa gaacgccggg aaatatgctt
     2401 ttattcatgt tgatttgctg gaaggcgcgt caaataaaga agtcgtgatt cagtttttga
     2461 aactggtgac cgaggcggac ggcattatca gtaccaaagc ctcaatgttg aaagcggcaa
     2521 gagcggaagg ttttttctgt attcatcgcc tgtttattgt tgattcaatt tcgtttcaca
     2581 acattgataa gcaagttgcg caatcgaatc cggattgtat tgagatcctg ccaggctgta
     2641 tgcccaaagt gctgggctgg gtgacagaga aaatccgcca accgctgatt gccggtgggc
     2701 tggtgtgcga tgaagaagat gcgtgtaatg cgattaacgc gggtgtcgtg gcgctttcca
     2761 ccacgaatac cggggtctgg acgttagcga aaaaattact ttgacgggat aatcgtaacc
     2821 aattgaattt ggtttgattt gtaggccgca cgccacatcc gacattcagc gcctgatgcg
     2881 acgcttgacg cgtcttatca ggcctacaag tccccaggcc agaaccgtag gcaggatcat
     2941 ccggcaacac attattggca atctgcgtga atgtttgtca ccagtgcctc cagcaccact
     3001 ttccagtcat caaccacgcc aacatctgcc tgtgaaaata ccgcagcgct ggcgtcatga
     3061 ttaatcgcca ccacaaactt gctattgcgt acgcccgcca ttaacgctgc tgcgccggaa
     3121 gcgccaacaa caatacacac ctcaggtgcc agcaaatgtc cggaaatacc aatcactttt
     3181 tcagcatcaa cgccaccgtt catcactcgc gcccgactgt agcccacttc ggctcccagc
     3241 ttttctgcca gcatagcgat ttcctggtta tctgcctctc cgccctgccc caccaccagc
     3301 acccgtctgg cctcagctaa tgggtcgcga gtaacatttt tcagatcctc tgtgctaacc
     3361 agccagtcgg gaagcgcacc cgggacaatg ttcagttgtt gtattccaga gggcaacgtg
     3421 gcgtttttcg ctgctcccgc ctgacgcgcc agagaaaggc atagcgggcg tttttctgtt
     3481 tgcagcgtcg ccgttagcgc atttccccag tgggatttgc gcacgcttac ggttggaata
     3541 tctaacgaag tcacctggca gatgcttgca ccatgtaaac gccaggccag ccgggtcgac
     3601 agttcatcgc caaacgttcc cggcggaaag agaacaacgt ccgcaggcgt tcgctgccac
     3661 tgttccacca acgcatccag cacctgttcc gccactacag gctgaggttc aatctgccag
     3721 tgcgccaggg tacaaccact gaaatcctgc gccgccagcc agcttgctat tgcggcattc
     3781 tcctgattta ttgtcacaat tgcgatattc atggctgcat cctttggcgt aaatagtcct
     3841 gccatagctt ttgagctttt tctgctaccg tttgcccatc aatcagcgtc gctccgcgtc
     3901 gttgctctgc tcttgccagt tgcagacact gcatcgctgg catttcggca gcaacagttt
     3961 tacggataat ttctgctttc cccgcagcca ttcgctggcg cattcccggt actggcaagg
     4021 ccacttcacc gcactgacgc acggcaatca ccgcaggcaa gcgaacccgg caacaacgca
     4081 acccatgttc agtacgctgt tcgagggtga taaacagcgc gtcgagagtg aaacgctcca
     4141 cctgggtgaa gcaggtccag cccagcattt ccgccagcaa aaatggcgtt tgcccattct
     4201 gcccttcgct gctttggctg ccggtaatta tcagatccag cggattctga tgctgccatt
     4261 cggcaatatg gcgagcgaca aattccggcg caaagcgcag atctgccgcc gtctccagca
     4321 atacagcttc ctcaaaccca agagccatga gatagcgcag ccagtgcaat gcccgttcat
     4381 cccccatgct caacgcggtt aaagacatcg gcgtaccgtt ttttctctgc gccaacagca
     4441 gcgcggcagc ggcctgttca tcagcaccga gtaaacttcg cagtagcgaa atatccggtc
     4501 cgcttttacc ctgagccgcc gcctgccact ctttttccgc cagcattccg gcatccggtt
     4561 cggctttaaa cgctaacaga atgtt
//
LOCUS       DAJTMC010000229         4560 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.229, whole genome shotgun sequence.
ACCESSION   DAJTMC010000229 DAJTMC010000000
VERSION     DAJTMC010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4560
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.229"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            101..850
                     /locus_tag="OO130_003914"
     CDS             101..850
                     /locus_tag="OO130_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000093946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HCR5367129.1"
                     /translation="MSSFLLGRFLHPGVFSLCVLLPLLASATTSHISFSYAARQRMQN
                     RARLLKQYQTHLKKQASYIVEGNAESRRALRQHNREQIKQHPEWFPAPLKASDRRWQA
                     LVENNHFLSSNHLHNITEVAIHRLEQQLGKPYVWGGTRPDQGFDCSGLVFYAYNKILE
                     AKLPRTANEMYHYHRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDGQFIESPRT
                     GETIRVSRLAEPFWQDHFLGARWILTEETIL"
     gene            1026..1523
                     /gene="rayT"
                     /locus_tag="OO130_003915"
     CDS             1026..1523
                     /gene="rayT"
                     /locus_tag="OO130_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HCR5367130.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSQLLTTQYQMLRNAIIKVKR
                     DRPFEINAWVVLPEHMHCIWTLPEGDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPVKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDVTDINAG
                     ERIIL"
     gene            complement(1747..3459)
                     /locus_tag="OO130_003916"
                     /pseudo
     CDS             complement(1747..3459)
                     /locus_tag="OO130_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            3431..4216
                     /gene="lafU"
                     /locus_tag="OO130_003917"
     CDS             3431..4216
                     /gene="lafU"
                     /locus_tag="OO130_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HCR5367131.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSITTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLDNKIIITGHIDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDSKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRTR"
BASE COUNT         1085 a         1201 c         1195 g         1079 t
ORIGIN      
        1 tgaaacctca atagtaatat gcaaataagc aattaataat cccgtttact cctccggtct
       61 tttaaaattc tcaccttcat tcagccggtt tatgtccttc atgtcttcgt tcttgctcgg
      121 cagatttctg catccaggcg tgttttcgtt atgcgttttg ctccctttac tcgccagcgc
      181 caccacatca catatctctt tcagctacgc cgcccgccag cggatgcaaa accgtgcgcg
      241 tttattaaaa cagtaccaaa cccatctgaa aaaacaggcc agctacattg tggaaggcaa
      301 tgccgaaagc agaagggcgc tacgccagca caaccgggag cagataaaac agcatccaga
      361 atggtttcct gcgccgctca aggcaagtga cagacgctgg caggcgttgg tagaaaacaa
      421 tcatttcctc agcagcaacc acctgcataa tatcaccgaa gtggcgattc accgtctgga
      481 gcaacagctt ggcaagcctt acgtctgggg cggcacgcgg cccgatcaag gcttcgactg
      541 tagcgggttg gttttttatg cctacaacaa gatccttgag gctaagctcc cgcgcacggc
      601 taatgagatg tatcactacc atcgggcaac cattgtggca aataacgacc tgcgccgggg
      661 agatttgctg tttttccata tccacagccg tgaaattgcc gaccatatgg gcgtgtatct
      721 gggagacggt cagtttattg agtcgccgcg cacgggggaa accattcggg taagtcggtt
      781 agctgaacct ttctggcagg atcatttttt gggcgcgcgg tggatattga cggaagagac
      841 gattttgtag gacggataag gcgtttacgc cgcatccggc ggtggtgcgc agatgcctga
      901 tgcgacgctg acgcgtctta tcatgcctac aaatctgtgc gtttttgcga gccgctttcc
      961 tgttattcca tcacctcatt gccgaccttt ccttttcttc ttaccgttga gaaaaaggag
     1021 tcgccatgtc tgaatatcgt cgttattaca tcaagggggg aacctggttt ttcacggtga
     1081 atttacgcaa tcgtcgaagc caactcttga ccacccagta ccagatgctc cgtaacgcca
     1141 ttattaaagt taagcgagac aggccttttg aaatcaacgc ctgggtcgtt ttgccagagc
     1201 atatgcattg catatggaca ttacctgaag gcgatgatga tttttcctct cgctggcggg
     1261 aaattaaaaa gcaatttacc catgcttgtg gattgaaaaa tatctggcaa ccacgttttt
     1321 gggagcacgc catccgcaat accaaagatt ataggcatca tgttgattat atttatataa
     1381 atccagtaaa gcatggttgg gtaaagcaag tgagtgattg gccattctca acgttccatc
     1441 gcgatgtcgc gcgagggtta tatcccatcg attgggcggg ggacgtaacg gatataaatg
     1501 ctggcgagcg tatcatttta taattatgcg cagatgcctg atgcgacgct ggcgcgtctt
     1561 atcatgccta caaacctgtg ccggatcggt aggccggata aggcgtttat gccgcatccg
     1621 gcggttatgc gcagatgcct gatgcgacgc tggcgcgtct tatcatgcct acaaacctgt
     1681 gctggatcgg taggccggat aaggcgttca cgccgcatcc ggcagttgtg caccaatgct
     1741 ggatacttac atcaacgccc ccataatctt caactccagc tcgtccggca cttcgttata
     1801 cgacagcaca tgcagccccg gcgcaaacaa ccttgcataa cgcgccagca aagggcgcag
     1861 ctgcggtggc accagcagca ccgggtcttt ccccgccgct ttcatctgct ctttcacctg
     1921 tggcatcgta ctctggaact ggttgagcat attcgggtcc actggcacgc tgtcgagcat
     1981 cactttaccg ccctgttgcg cctgattcac taggttagtc agcagatttt ccagctcatt
     2041 attcagggta tacaccgtca gctcctgctt gcgaacgaac ggatgggtaa tgctgcgccg
     2101 cagcgccagg cgcacatcgg ctgccagcag aatatgatct ttggtcaccg cgctactggc
     2161 gaccagcacg gtggcgatgg tgacgatatc gcgcagggaa acgccttcgg tcagcagcgc
     2221 ccggtacact ttcagcaact ggctgtaatt gagcaccgcg cttaaatcct ccgccaggcg
     2281 cggtgccatc gacgacaaac ggttatgcaa ctgcgtaata tcgtcatagt taaataaatc
     2341 aggaatatag ctgcgcacaa tcttatttac atgggtagca atcacgctgg cgctgtcgat
     2401 cacctgatac cccatattca gcaccttcgc tttctgcgca ggctggatcc aggtcaccgg
     2461 catcccgtac gccgggtcgt tccccagcac gccgtctatc tcgccgtagg tttcgctgga
     2521 gggctgggcc atcagtttat ccgccggaat atccgcttca tcagccttaa tgccgttgat
     2581 gaaaatagcg tactggctgg gcttaaggcg gaagttttcc cgaatgcgga tctccggcag
     2641 cagcacgccg ttgccgtcgg atatcacctg tcgcacgccg cgaatacgct gggtgagcgg
     2701 gttgccctgg gctttatcga ccagcgccac cagcttgtag ccgaggctta aactgatagg
     2761 ctcgataagc ggaatggttt cccaactgac ctgttgctcg ctggtttcgg tgatggtgcg
     2821 ggtcagcgtt tcgagacttt tctcttccgc ttccgccgcc tgcgggcgtt tgctcatccg
     2881 ccagccggta aagccaagca gggcgctgaa cagcaaaaac ggcagatgtg gcattcccgg
     2941 cactaccgcc agtacgaaca taattccggt agcggtgtag agcaccgatg ggcttgccag
     3001 caactggtta cgcacgtcat gggcgatatc gccgttgtcg ctgacgcggg tgaggataat
     3061 cgccgccgcg gtggagagta gcagggaagg gatttgcgcc accagcccgt cgccgatggt
     3121 catcagcaca tactgctgga aggcggcgtc ggcgctcagg ttgtatttga agatcccgat
     3181 acagacgccg ccgatcaggt tgatcgccag aatcatcatc ccggcgatag catccccgcg
     3241 cacaaatttc gatgccccgt ccatcgcgcc gtagaaatcg gcctcgctgg caacatcttt
     3301 acgtcgggtt tgtgcctgcg cctgattgat caacccggca ttaagatcgg cgtcaatcgc
     3361 catctgctta ccgggcatcg catccagggt aaagcgggct gaaacctcgg aaatacgctc
     3421 ggcccctttg gtgacgacga taaagttgat tgtcaacagc gtcagtaaat ccgagcgtga
     3481 aagcattatc gccgcgctgc acgggcagtc gatttttaac ggtggtgggc tgtcgccgtt
     3541 aaataaaatc agcccgtctc atccgccaaa acctgccacc gtggcggtgc cggaagaaac
     3601 ggagaaaaaa gcgcgcgacg tcaatgagaa aacggcgctg ctgaagaaga aatccgccac
     3661 cgagcttggc gagctggcaa ccagtatcac caccattgcc cgtgatgcgc atatggaagc
     3721 gaatctggag atggagattg ttccccaggg attacgcgtg ctgattaaag acgaccagaa
     3781 tcgcaatatg ttcgaacgcg gcagcgcgca gattatgccg ttctttaaaa cgctgctggt
     3841 ggagctcgcg ccagttttcg actcgctcga taacaaaatt attattaccg ggcatatcga
     3901 tgcgatggct tacaaaaaca atatctacaa caactggaac ctttcgggtg accgcgcgct
     3961 ttcggctcgt cgggtgctgg aagaggccgg aatgccggaa gataaagtga tgcaggtaag
     4021 cgcaatggcg gaccagatgc tgctggattc caaaaatccg caaagcgcgg gcaaccggcg
     4081 cattgagatt atggtgctga ccaaaagtgc gtccgatacg ctgtatcaat actttggtca
     4141 gcatggggat aaagtggtgc agccgctggt gcaaaagctg gataagcagc aggtgctttc
     4201 gcagcgaacg cgttaaatgc tgaatcttta cgcatttctc aaactctgaa atcactgtat
     4261 actttaccag tgttgagagg tgagcaatgc gtaaaatcat gattttcact tcctaaaaca
     4321 ccccttataa tctggtcata gagtcattat agcactggat gaatatacag gtgaggaagg
     4381 tttacagtta cattcgcttt tcgtcaacga aacaagcttt tggtgatagt catcgaagac
     4441 agagtaaggc catacaggat tggttagcca gccatccaga ccacatccta gacgaatctc
     4501 ttagttttga agatttagga cgttcagcct ttcacggtga ccatctaaaa gaaggtggag
//
LOCUS       DAJTMC010000230         4439 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.230, whole genome shotgun sequence.
ACCESSION   DAJTMC010000230 DAJTMC010000000
VERSION     DAJTMC010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4439
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.230"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            <1..1088
                     /gene="nadB"
                     /locus_tag="OO130_003918"
     CDS             <1..1088
                     /gene="nadB"
                     /locus_tag="OO130_003918"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HCR5367132.1"
                     /translation="VGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPDISSGD
                     GIAMAWRAGCRVANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTRFMPDF
                     DERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLGLGIDL
                     TQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSLLECLV
                     YGWSAAEDITRRMPDAHGVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDYVGIVR
                     TTKRLERALRRINMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRKESRGL
                     HFTLDYPELLTHSGPSILSPGNHYINR"
     gene            complement(1073..1810)
                     /gene="trmN"
                     /locus_tag="OO130_003919"
     CDS             complement(1073..1810)
                     /gene="trmN"
                     /locus_tag="OO130_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HCR5367133.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWLTQQTVRFDLIISNPPYYQQGVECAIPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            1942..3276
                     /gene="srmB"
                     /locus_tag="OO130_003920"
     CDS             1942..3276
                     /gene="srmB"
                     /locus_tag="OO130_003920"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HCR5367134.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIEELRPKTRTPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            complement(3485..4366)
                     /locus_tag="OO130_003921"
     CDS             complement(3485..4366)
                     /locus_tag="OO130_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5367135.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKGSDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     LSIESIKRCVAANIGVSYLPRFAVEKELESGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHTFIQCIEECFLPG"
BASE COUNT         1126 a         1209 c         1126 g          978 t
ORIGIN      
        1 ttgtaggcgc gtgggtatgg aaccgaaata aagaaacggt ggaaacctgc cacgcaaaag
       61 cagtggtact ggcaaccggc ggtgcgtcga aggtgtatca gtacaccacc aatccggata
      121 tttcttctgg cgatggcatt gctatggcgt ggcgcgcggg ctgtcgggtt gccaatctcg
      181 aatttaatca gttccaccct accgcactgt atcacccaca ggcacgcaat ttcctgttaa
      241 cggaagcact gcgcggcgaa ggcgcttatc tcaagcgccc ggatggcacg cgttttatgc
      301 ccgattttga tgagcgcggc gaactggccc cgcgcgatat tgtcgcccgc gccattgacc
      361 atgaaatgaa acgcctcggc gcagattgta tgttcctcga catcagccat aagcccgccg
      421 attttattcg ccagcatttc ccgatgattt atgaaaaatt gctcgggctg gggattgatc
      481 tcacacaaga accggtaccg attgtgcctg ctgcacacta tacctgcggt ggtgtaatgg
      541 ttgatgatca tgggcgtacg gacgtcgagg gcttgtatgc cattggcgag gtgagttata
      601 ccggcttaca cggcgctaac cgcatggcct cgaattcatt gctggagtgt ctggtctatg
      661 gctggtcggc ggcagaagat atcaccagac gtatgcctga tgcccacggc gtcagtacgt
      721 taccaccgtg ggatgaaagc cgcgttgaga accctgacga acgggtagta attcagcata
      781 actggcacga gctacggctg tttatgtggg attacgtcgg cattgtgcgc acaacaaaac
      841 gtcttgagcg cgccctgcgg cgtatcaata tgttgcaaca ggaaatagac gaatattacg
      901 cccacttccg cgtgtcgaat aatttgctgg agctgcgtaa tctggtgcag gttgcggaat
      961 tgattgttcg ctgtgccatg atgcgcaagg agagtcgtgg cctgcatttc acactggatt
     1021 atccggaact gctcacccat tccggtccgt cgatcctttc ccccggcaat cattacataa
     1081 acagataaaa agcctgggtc agcgccgtat acgcttcgga atagttctgg tctggcccac
     1141 gaatgactaa gcgatcgcta aagcattctc ccgcctgcgg ggagaatgcc agcagcaccc
     1201 gatgcggcag tcgcgcttcg ttttccgcca catccgtccg caaacgtaaa tgccagccca
     1261 tgcttaatgc cagctccgta aaaccattac caatctgctc tggcagcact acgcagaaaa
     1321 atccctcttc ggtaatgcac tccgccgcac aggtcagcag cgatgggtga tcaagcgtag
     1381 tggtatagcg agcctgttcc cgttgaggga tcgcgcactc tactccctgc tgatagtaag
     1441 gtgggttact gatgattaaa tcgaagcgta ctgtctgctg tgtgagccac tgctgaatat
     1501 ccgccgtatg gacgttaatc cgctctgccc acggggactg gttgatattt tcctgcgcct
     1561 gcgcggcagc ttcactttcc agctcaacag catcaattat cacgctgtca ctggttcgct
     1621 gcgccagcat cagtgccagc aacccgctac ccgcgccgat atcaaggcaa cgtttcaccc
     1681 cagccaccgg tgcccatgcg cccagcaaaa tgccatccgt tcccactttc atcgcacagc
     1741 gatcgtgagc aacaaaaaac tgtttaaaag taaatccatt acgacgaagc acggatgtag
     1801 actgtgacat gaaaataaaa ccttgcagga aaaacggcga tagcaccggg tgagaacaat
     1861 acccgagaag cgatatccat acaaacagat gaagattgca gccgtaacgt ctataatcag
     1921 cgccccacac agaggtagaa catgactgta acgacttttt ccgaacttga actcgacgaa
     1981 agcctgctgg aagccctcca ggataaaggt ttcactcgcc cgaccgccat tcaggctgcc
     2041 gccattccgc ctgcgctcga tggccgtgat gtactcggtt ctgcgccgac aggcaccggt
     2101 aaaacggcgg cgtatctgct gccagcgttg cagcacctgc tcgatttccc gcgtaagaaa
     2161 tccggtccgc cgcgtatttt gatcctcacc ccaactcgcg agctggcgat gcaggtggcc
     2221 gatcatgccc gcgaactggc gaaacatacg catctggata tcgccaccat taccggcggc
     2281 gtagcctata tgaaccacgc ggaagtgttc agcgaaaatc aggacatcgt ggtcgccacg
     2341 accggacgtc tgctgcaata cataaaagaa gagaacttcg attgccgcgc ggttgaaacg
     2401 ctgatcctcg acgaagcaga ccgtatgctg gatatgggct tcgctcagga tatcgaacat
     2461 attgctggcg aaacgcgctg gcgtaaacag accctgctct tttcggcaac gctggaaggc
     2521 gatgcgattc aggactttgc cgagcgtctg ctggaagatc cggtggaggt ttctgccaat
     2581 ccgtccaccc gtgagcgcaa aaaaattcat cagtggtatt accgcgccga tgacctggaa
     2641 cataaaaccg cattgctggt gcatctgtta aaacagccgg aagcgacccg ctcaattgtg
     2701 tttgtgcgta agcgtgaacg tgtgcatgag ctggcaaact ggctgcgcga agcgggcatc
     2761 aacaactgct atctcgaagg tgagatggta cagggcaagc gtaatgaagc gattaaacgc
     2821 ctgaccgaag gccgcgtaaa cgtactggta gcaaccgatg ttgccgcgcg cggtatcgac
     2881 attccagacg tcagccacgt ctttaacttc gatatgccgc gcagtggtga tacttatttg
     2941 caccgtatcg gacgtaccgc gcgcgccggt cgtaaaggca ccgcgatttc gctggtggaa
     3001 gcccatgacc atctgctgct tggcaaagtt ggccgttata ttgaagagcc gataaaagct
     3061 cgcgtcattg aggaactacg cccgaaaacg cgtacgccaa gcgagaagca gaccggcaag
     3121 ccatcgaaga aagtactggc aaaacgcgcc gagaagaaaa aagctaaaga gaaagagaag
     3181 ccgcgggtga aaaaacgcca tcgcgacacc aaaaatattg gtaagcgccg taaaccgagc
     3241 ggaacgggcg tgccaccgca aacgacagaa gagtaatctt aattccgggt ttaagcccgg
     3301 aattaaaagt gctggcaaac gcaaaactgc ctgatgcgct acgcttatca ggcctacatg
     3361 gctcatgcaa tatattgaat ttgcacgatc ttgtaggccg gataaggcgt tcacgccgca
     3421 tccggcatga acaaaacgca cttcgtcgac aagttgggtg ccgggtttaa gcccggcatt
     3481 atttttatcc cggcaaaaaa cactcttcaa tacactgaat aaaagtgtgc atcgccgggc
     3541 taaccgcttt tccagcatgg tgtgcgcaca tagccgtaat ggtctgcgac tgttcgccaa
     3601 aaggcaattc aattaattct ccgctctcaa gctctttttc caccgcaaag cggggcaagt
     3661 aactgacacc gatattcgcc gcgacacaac gtttgatact ttcaatgctt aacagctcaa
     3721 tggtgttttc caccgtgatc cgccgctgac gcagcgtgct ctcaaatatc tgccggaaga
     3781 cacattgcgg ttcgttgata ataaagctac aggcgttatg tcttcccggc tcagtaaaat
     3841 cgacatctgc aatttgcggt gaagccacca gcaccagcga ttgttcaccc aactctcgtc
     3901 ggttcagggc atcatcattc cctacgcgat aaaagacgcc gacatcggcc tcatcattca
     3961 gtagcgcatc acggatcacg taacagttca gcgactgcaa cgataaacgc acttttggtg
     4021 cgcgttgccg aaaacgctgc aacacctgcg gcattcggta agagagcaac gtttcgcccg
     4081 aaacaacgcg tagttccccg tccggatctg accccttttt ggcagcctca cgaagcgtat
     4141 ccatcactcg ggtaagttca taaatgtgcg gcagcaactt ttttccttcg cgggtaaggc
     4201 acatccgtcg gccaattttc tcaaataact ggactgaaaa ctcctgctca agctgctgaa
     4261 tatgaaaagt caccgtcgat tgtgtacagc acaatttttg cgaagctcgc aaaaaggaac
     4321 cctcttccac cacggtttta agcgtaataa aacggcgcag atccataacc ccgaacctat
     4381 cgaaaatatc gaatctagaa tataaaaaca ttcatttttt taaatgttct gtgtcgggt
//
LOCUS       DAJTMC010000231         4429 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.231, whole genome shotgun sequence.
ACCESSION   DAJTMC010000231 DAJTMC010000000
VERSION     DAJTMC010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4429
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.231"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            127..456
                     /locus_tag="OO130_003922"
     CDS             127..456
                     /locus_tag="OO130_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HCR5367136.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            704..949
                     /gene="nrdH"
                     /locus_tag="OO130_003923"
     CDS             704..949
                     /gene="nrdH"
                     /locus_tag="OO130_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HCR5367137.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            946..1356
                     /gene="nrdI"
                     /locus_tag="OO130_003924"
     CDS             946..1356
                     /gene="nrdI"
                     /locus_tag="OO130_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HCR5367138.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            1329..3473
                     /gene="nrdE"
                     /locus_tag="OO130_003925"
     CDS             1329..3473
                     /gene="nrdE"
                     /locus_tag="OO130_003925"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HCR5367139.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     MATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAYAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDATLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENVQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPVAGRINMSNLCSEIL
                     QVNCASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYAITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            3483..4397
                     /gene="nrdF"
                     /locus_tag="OO130_003926"
     CDS             3483..4397
                     /gene="nrdF"
                     /locus_tag="OO130_003926"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HCR5367140.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPYALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYSDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSRIGN"
BASE COUNT         1093 a         1131 c         1131 g         1074 t
ORIGIN      
        1 cgggcctacc ttacctgatt gcgcattcac aacgctttca gaaaagtcca taaagccgcg
       61 atgaacctca gttaagaaaa ttatggtcta cactgaaaat tacatcgaat tctaatgggg
      121 gatcatatgt ttaaccgacc gaaccgcaac gacgttgatg atggcgtgca ggatatccag
      181 aatgatgtca atcaattagc tgacagcctc gaatctgtat tgaagtcctg gggcagcgac
      241 gccaaagggg aagctgaagc cgcacgcagt aaagctcagg cgttgctgaa agaaaccagg
      301 gcgcgaatgc atggtcgtac tcgcgtccag caagccgcgc gcgatgccgt tggctgcgcg
      361 gattcttttg ttcgtgaaag accctggtgt agcgtgggta cagcagctgc ggtaggtatt
      421 tttatcggcg cactgttaag catgcgcaaa tcgtagtgca aaaatgataa taaatacgcg
      481 tctttgaccc cgaagcctgt cttcggggtt tctttttgcc tggtgaatca caaaaatccc
      541 cctcccccgt cacgctcata tccagggtaa tttcgatcac tatttgctat atattgtgta
      601 gttgaatctt ttttcaacta catctagtat ctctgtatca acagagagac aacccgacgc
      661 gtatcatcgc gccgtatctt cattttaaac ggaaatacga atcatgcgca taactattta
      721 cactcgtaac gattgcgttc agtgccacgc caccaaacgg gcgatggaaa accgggggtt
      781 tgattttgaa atgattaatg tcgatcgcgt tcctgaagca gcggaagcgt tgcgtgctca
      841 gggctttcgt cagttgccgg tagtgattgc tggcgatctt agctggtctg gtttccgtcc
      901 ggacatgatt aaccgtctgc atccagcgcc acacgcggcc agtgcatgag ccagctcgtc
      961 tacttctcca gcagctccga aaacacgcag cgttttatcg aacgtttagg tctgcccgcg
     1021 gtgcgcatcc cgctcaatga gcgggaacgg attcaggtag acgagcctta catcctgatc
     1081 gtgccctctt acggcggcgg cggtacggct ggcgcggtgc cacgacaggt aattcgcttt
     1141 ttaaacgacg agcacaaccg ggcgttgctt cgcggcgtta ttgcttctgg taatcgcaac
     1201 tttggtgagg cgtatggccg cgccggagat gtgattgccc ggaaatgcgg cgtgccgtgg
     1261 ctgtaccgtt ttgaactcat gggtacgcaa agcgatatcg aaaacgttcg taaaggagta
     1321 accgaatttt ggcaacgaca accgcagaat gcctgacgca ggaaacgatg gattaccacg
     1381 cgctgaatgc gatgcttaac ctctacgata gcgcaggtcg cattcagttc gataaagacc
     1441 gccaggccgt tgacgccttt atggcgacgc atgtgcgtcc gaacagtgtg accttcagta
     1501 gccagcagca gcgcctgaac tggctggtca acgaaggtta ctatgatgaa agcgttctta
     1561 atcgctactc tcgcgacttt gtcattacgc tgtttgccta cgcacacacc agcggttttc
     1621 gtttccagac attcctcggg gcatggaagt tttacaccag ctatacgttg aagacattcg
     1681 acggtaaacg ttatctggaa gattttgccg atcgagtcac gatggtggcg ctgacgctgg
     1741 cacaaggcga tgcgacgctg gcgttgcaac tgaccgatga aatgctgtca ggacgctttc
     1801 agccagccac gccgacattc ctcaactgcg gtaagcagca gcgcggcgaa ctggtttcct
     1861 gttttctgct gcgtattgaa gacaatatgg agtcgattgg ccgggcggta aattccgcac
     1921 tgcaactgtc gaaacgcggc ggcggcgtgg catttttgct gtcgaatctg cgagaagcgg
     1981 gcgcgccaat taaacgtatt gaaaatcaat cttctggcgt aattccggtg atgaaaatgc
     2041 tggaagacgc attttcctat gccaaccaac tcggcgcgcg tcagggggca ggtgcagtct
     2101 atttacatgc tcatcatccc gatattctgc gttttctcga tacaaaacgg gaaaatgccg
     2161 acgaaaaaat ccgcattaaa acactgtcgc tgggcgtggt gatcccggat atcactttcc
     2221 atctggcaaa agagaatgtg cagatggcgc tgttttcgcc ctatgacgtt gagcgagttt
     2281 atggcaagcc gtttgccgat atcgccatca gcgaacacta tgacgaactg gttgccgatg
     2341 aacgcatccg caaaaaatac ctcaacgccc gtgatttctt ccagcggctg gcagaaatcc
     2401 agtttgagtc cggctatccc tacatcatgt atgaagacac ggtaaaccgt gctaaccctg
     2461 tcgccgggcg cataaatatg agtaatctct gctcagagat tttgcaggtc aactgtgcct
     2521 cagagtatga cgagaatctc gactatgccc gcacaggcca tgatatctcc tgcaatttag
     2581 gttcgttgaa tattgcgcac accatggatt cccccgattt tgcccgcacg gtagagactg
     2641 ccgtgcgcgg tttaacggcg gtgtcagata tgagtcatat ccgcagcgtg ccgtccatcg
     2701 aagccggaaa tgccgcctca cacgccatcg gactggggca gatgaattta cacggctatc
     2761 tggcgcgaga aggcatcgct tatggttcgc cggaagcact ggatttcacc aatctctatt
     2821 tctatgccat cacctggcac gcactgcgta cctcgatgtt gctggcgcgc gaacgcggtg
     2881 aaaccttcgc cgggttcaaa cagtcacgtt atgccagtgg tgaatatttt agccaatatc
     2941 tgcaaggtaa ctggcagccg aaaacggcga aagttggcga actgtttgcc cgcagcggta
     3001 ttacgttacc tacccgtgag atgtgggcgc aactgcgcga cgacgtgatg cgctacggca
     3061 tttataacca gaatcttcag gcggtgccgc caaccggttc tatctcttat atcaaccatg
     3121 ctacgtcgag tattcatccg attgtggcga aagtagagat acgcaaagag ggcaaaacag
     3181 gacgcgttta ctaccctgcc ccgtttatga ctaacgagaa tctggcgctg tatcaggatg
     3241 cttacgaaat cggcgcagaa aagatcatcg acacctacgc cgaagcgact cgccatgtcg
     3301 atcaggggct gtcgctgacg ctgtttttcc ccgataccgc caccactcgc gatatcaaca
     3361 aagcgcagat ttacgcctgg cgcaagggta tcaaaacgct ctattacatt cgcctgcgtc
     3421 agatggcgct ggaaggcact gaaattgaag gctgcgtctc ctgtgcactt taaggaatat
     3481 ctatgaaact ctcacgtatc agcgccatca actggaacaa gatatctgac gataaagatc
     3541 tggaggtgtg gaatcgcctg accagcaatt tctggctacc agaaaaggtg ccgctgtcga
     3601 acgatattcc tgcctggcag acattaactg tcgtagaaca acaactgacg atgcgcgttt
     3661 ttactggcct gacgctgctc gacacgctgc aaaatgttat cggcgcgcct tcgctgatgc
     3721 cctatgcact cacgcctcat gaagaagcgg tattatcgaa tatcagcttt atggaagcgg
     3781 ttcatgcccg ctcttacagt tcgattttct cgacgctgtg tcagaccaaa gatgtcgatg
     3841 ccgcctacgc ctggagtgaa gaaaacgcac cgttgcagcg aaaagctcag attattcagc
     3901 aacattatcg cggtgatgat ccgctgaaaa agaaaatcgc cagtgtgttt cttgaatctt
     3961 ttttgttcta ttccggtttc tggctgccga tgtatttttc cagccgcgga aagctgacca
     4021 ataccgcaga tctgatccgt ctgattatcc gcgatgaagc agtccacggt tactacatag
     4081 gctataaata tcagaaaaac atggaaaaga tatctctggg acaacgtgaa gagttgaaga
     4141 gtttcgcctt cgatttgttg ctggaactct acgacaacga gttgcaatac agcgatgagc
     4201 tgtacgccga aaccccgtgg gctgacgatg tgaaagcgtt tctctgttac aacgccaata
     4261 aggctttgat gaatctgggc tacgaaccgt tatttcccgc agaaatggcg gaagtgaatc
     4321 cggcaatcct cgccgcgctt tcgccgaatg ccgatgaaaa tcacgatttc ttttccggtt
     4381 cacgtatagg aaattgaaaa actaagcgat acccctgtcc gcgccttat
//
LOCUS       DAJTMC010000232         4422 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.232, whole genome shotgun sequence.
ACCESSION   DAJTMC010000232 DAJTMC010000000
VERSION     DAJTMC010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4422
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.232"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            157..765
                     /locus_tag="OO130_003927"
     CDS             157..765
                     /locus_tag="OO130_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HCR5367141.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            863..2254
                     /gene="selA"
                     /locus_tag="OO130_003928"
     CDS             863..2254
                     /gene="selA"
                     /locus_tag="OO130_003928"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HCR5367142.1"
                     /translation="MTTETRSLYSQLPAIDRLLHDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRDSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            2251..4095
                     /gene="selB"
                     /locus_tag="OO130_003929"
     CDS             2251..4095
                     /gene="selB"
                     /locus_tag="OO130_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HCR5367143.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPSFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPGREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETAHAGQRIALNIAGDAEKEQINRGDWLLADAPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQNDSEALAVHLQRGAVNLPDFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFSDEPWW
                     VRDLAKETGTDEQAMRLTLSQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
     gene            4285..>4422
                     /locus_tag="OO130_003930"
     CDS             4285..>4422
                     /locus_tag="OO130_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HCR5367144.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDSMLTK
                     LG"
BASE COUNT         1038 a         1088 c         1245 g         1051 t
ORIGIN      
        1 aagtgagata tccatcagag tgaaataatt aggaaaatat ttatatcacc atgataaata
       61 tgtgatttat ttggaagtaa caaaccatca acattttctg ccctttttcc catcaccagg
      121 ccatactgat atttttcctc gcacaaagga ctcggcatga aactcgtcgg tagctacacc
      181 agcccgtttg tacgcaaact ttctattctg ttgttagaaa agggcataac tttcgaattt
      241 attaatgaac tgccctataa cgcggacaac ggcgtggcgc aatttaaccc gttaggaaaa
      301 gtgccggtgc tggtgaccga agagggcgaa tgctggtttg attcgccgat catcgctgaa
      361 tatattgagt taatgaatgt cgctccggcg atgttgccgc gcgatccgct ggagtcgttg
      421 cgggtgcgca aaattgaggc gctggcggat ggcattatgg atgccgggct ggtatcggtg
      481 cgtgaacagg cgcgtccggc ggcgcagcag tctgaagatg aattgttacg ccagcgggag
      541 aaaatcaacc gcagtctgga tgtgctggaa ggatatctgg tcgatggcac actcaaaacc
      601 gatacggtca atctggcgac tatcgccatt gcctgtgctg tcggatatct caatttccgc
      661 cgcgttgcgc caggttggtg tgtcgaccgc ccacacttag tcaaactggt cgaaaacctg
      721 tttagccgcg aaagttttgc tcgcaccgaa ccgccaaagg cttgatgcgc gatatgtcct
      781 cctgacccat ctcacgttac aatccgtggt tatgttaaac gcccttctcc atgtgagagg
      841 gccttgatta gccaggtttc ctatgacaac cgaaacgcgt tccctctata gtcaacttcc
      901 ggctattgat cgcttattgc acgatagctc cttcctttct ttgcgtgata cttatggtca
      961 cacccgcgtg gtggaattgt tgcgtcagat gctcgacgaa gcgcgagaag tgattcgtga
     1021 cagccagacg cttcctgcgt ggtgtgaaaa ctgggcgcaa gaagtcgatg cccggttgac
     1081 gaaagaggcg cagagcgcgc tgcgtccggt gatcaacctg acgggaaccg tgctgcatac
     1141 caaccttgga cgagctttac aggcggaagc cgcggtggaa gccgttgcgc aggctatgcg
     1201 ttcgccagtg accctcgagt acgatctgga cgacgctgga cgcggacatc gcgatcgggc
     1261 gctggcgcaa ctgctgtgcc gtattacggg ggcggaggat gcctgtatcg tcaataacaa
     1321 tgcggcggcg gtgttattga tgttggcggc cactgccagc gggaaagagg tggtagtatc
     1381 tcgcggcgaa ttggtggaga ttggcggcgc gtttcgtatt cccgatgtta tgcgtcaggc
     1441 aggctgcacc ctacacgaag tagggaccac caaccgcacg cacgcgaatg attatcgtca
     1501 ggcggtgaat gaaaataccg cactgttgat gaaagtacat accagtaact acagcattca
     1561 ggggttcacc aaagcgatag atgaagcgga actggtggcg ctcggcaaag agctggatgt
     1621 tcccgtagtg actgatttag gcagtggctc gctggtcgat cttagccagt acggtttgcc
     1681 gaaagagcca atgccgcagg agttgattgc ggcgggcgtc agtctggtga gcttctccgg
     1741 cgacaagttg ttaggcgggc cgcaggcagg gattatcgtt ggtaaaaaag agatgatcgc
     1801 ccgcctgcaa agccacccgc tgaagcgtgc attacgcgcg gataaaatga ccctcgcggc
     1861 gctggaagcc acgttgcgtc tttatttaca ccctgaagct ctgagtgaaa aattaccgac
     1921 cctgcgcctg cttacccgca gcgcagaggt cattcaaatc caggcacaac gtttacaggc
     1981 tccccttgcc gcacattacg gcgcggagtt tgcggtacag gttatgccat gtctttcgca
     2041 gattggcagt ggttcgctgc cggttgatcg cctgccgagc gcggcattaa cgtttacacc
     2101 ccatgatgga cgcggtagcc accttgagtc attagccgcc cgctggcgtg aattgccagt
     2161 gccggtgatt ggtcgtattt atgacggacg attgtggctg gatttacgct gccttgaaga
     2221 tgagcaacgg tttttggaga tgttgttgaa atgattattg cgactgccgg acacgttgac
     2281 cacggcaaaa caaccttatt gcaggcgatt actggcgtaa atgctgaccg tctgccggaa
     2341 gaaaaaaagc gcggcatgac catcgatctc ggctatgcct actggccgca gccggatggt
     2401 cgcgtgccta gttttatcga cgtacccggt catgaaaagt ttctttccaa catgctggcg
     2461 ggcgttggtg gtatcgatca cgcgctgttg gtggtggcgt gcgatgacgg cgtgatggcg
     2521 cagacccgtg agcatctggc gattttgcag ctgaccggta acccgatgct gacagtggcg
     2581 ctgaccaagg ccgatcgcgt ggacgaagcg cgtgttgatg aggttgaacg ccaggtaaag
     2641 gaggtgctgc gggaatacgg ttttgctgag gcaaaactgt ttatcaccgc agcaaccgaa
     2701 ggtcggggaa ttgatgccct gcgcgagcat ctgcttcagt taccaggacg tgaacacgcc
     2761 agccaacata gtttccgcct cgcgatcgac cgcgcattta ccgtaaaagg tgccgggctg
     2821 gtcgtcaccg gtacggcgtt aagcggggaa gtgaaggtag gcgattcact ctggctgact
     2881 ggtgtaaata aaccgatgcg tgtacgtgcg ctgcatgcac aaaaccagcc aacagaaacc
     2941 gcccatgccg ggcagcgtat cgcgcttaac atcgcgggtg atgcggaaaa agagcagatt
     3001 aaccgtggcg actggctgct tgccgatgcg ccgccagagc cgttcacacg ggtgattgtc
     3061 gagcttcaaa cccatacacc gctgacccag tggcagccgc tgcatattca ccacgccgcc
     3121 agccacgtca cgggacgcgt ttcactgctg gaagataacc ttgctgaact ggtcttcgac
     3181 accccgttat ggctggcaga taacgaccgc ctggtattgc gcgatatctc tgcccgcaac
     3241 acgctggccg gagcgcgcgt cgtgatgctt aacccgccgc gtcgcggtaa acgtaaacca
     3301 gagtatctgc aatggctggc ttccctcgcc agggcacaga atgatagcga agcgttagcc
     3361 gttcacctgc aacgcggcgc ggttaatctt cctgatttcg cctgggcgcg ccagctcaac
     3421 ggcgaaggga tgcgcgaact gctgcaacag cctggctata ttcaggctgg ttatagcttg
     3481 ttgaatgcgc cggttgccgc tcgctggcaa cggaaaattc tcgacacatt agcgacttat
     3541 catgagcaac atcgcgatga acctgggcct gggcgcgaac gtctgcgacg tatggcgttg
     3601 ccaatggaag atgaagcgct agtactgttg ctgattgaaa agatgcgcga aagcggcgac
     3661 atcctcagtc atcacggttg gctgcatctg ccggatcaca aagcgggctt cagcgaagag
     3721 cagcaggcca tctggcaaaa agcagagccg ctgtttagtg acgagccgtg gtgggtgcgt
     3781 gatctggcaa aagagacggg taccgacgag caggcaatgc gcttgacgct aagccaggcg
     3841 gcgcagcaag gaataattac cgcgatcgtc aaagatcgtt attaccgtaa cgatcggatt
     3901 gtcgaatttg ccaatatgat ccgcgatctc gatcaggagt gtggttcaac ctgcgcggcg
     3961 gatttccgcg atcgcttagg cgtaggccga aagctggcaa ttcagattct ggaatatttt
     4021 gaccgcattg gctttacgcg tcgtcgtgga aatgatcatt tattgcgcga cgcattatta
     4081 tttccggaaa aataaggaaa tgattaatta atttttaaaa taaattaata caaatttctt
     4141 atgattttaa aaaaagcaca ttgtttaatg aatacaatgt gctttttatt agattaattt
     4201 tgataattaa taataatgat catgttttcg aatgaaaatc ctcagtaagc ttcccaccct
     4261 tttttacact ttcaggagtg tgttatggca tcttcaactt tctttattcc ttctgtgaat
     4321 gtcatcggcg ctgattcatt gactgatgca atgaatatga tggcagatta tggatttacc
     4381 cgtaccttaa ttgtcactga cagtatgtta acgaaattag gt
//
LOCUS       DAJTMC010000233         4402 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.233, whole genome shotgun sequence.
ACCESSION   DAJTMC010000233 DAJTMC010000000
VERSION     DAJTMC010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4402
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.233"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            228..600
                     /locus_tag="OO130_003931"
                     /pseudo
     CDS             228..600
                     /locus_tag="OO130_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1162..2109)
                     /gene="icsP"
                     /locus_tag="OO130_003932"
     CDS             complement(1162..2109)
                     /gene="icsP"
                     /locus_tag="OO130_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease IcsP"
                     /protein_id="HCR5367145.1"
                     /translation="MKLKFLVLALCVPAIFTTHATTNYPLFIPDNISTDISLGSLSGK
                     TKERVYHPKEGGRKISQLDWKYSNATIVRGGIDWKLIPKVSFGVSGWTTLGNQKASMV
                     DKDWNNSNTPQVWTDQSWHPNTHLRDANEFELNLKGWLLNNLDYRLGLIAGYQESRYS
                     FNAMGGSYIYSENGGNRNKKGTHPSGERTIGYKQLFKIPYIGLTANYRHENFEFGAEL
                     KYSGWVRSSDTDKHYQTETIFKDEIKNQNYCSVAANIGYYVTPSAKFYIEGSRNYISN
                     KKGDTSLYEQSTNISGTIKNSASIEYIGFLTSAGIKYIF"
     gene            complement(3490..3642)
                     /locus_tag="OO130_003933"
                     /pseudo
     CDS             complement(3490..3642)
                     /locus_tag="OO130_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            3698..4390
                     /gene="ospZ"
                     /locus_tag="OO130_003934"
     CDS             3698..4390
                     /gene="ospZ"
                     /locus_tag="OO130_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     methyltransferase OspZ"
                     /protein_id="HCR5367146.1"
                     /translation="MISPIKNIKNVFPINTANTEYIVRNIYPRVEHGYFNESPNIYDK
                     KYISGITRSMAQLKIEEFINEKSRRLNYMKTMYSPCPEDFQPISRDEASIPEGSWLTV
                     ISGKRPMGQFSVDSLYHPDLHALCELPEISCKIFPKENSDFLYIIVVFRNDSPQGELR
                     ANRFIELYDIKREIMQVLRDESPELKSIKSEIIIAREMGELFSYASEEIDSYIKQMND
                     RFSQIKARMSVT"
BASE COUNT         1353 a          861 c          849 g         1339 t
ORIGIN      
        1 tgcgatctgg ttcaacaata tgcagatggt acacaacctc tttaaccagc ccatccggat
       61 tattcagagc ccttccgcaa tccggaataa aagtgctgtt ttaccgtgaa ccctctgaaa
      121 atcagcgagc agtgttttaa ccagccgacg ttcagagcgt actgacagac ggtggacaat
      181 ttgaataaac agttcaatca gaccgtagat gctctcccgt tggcgacatg ctgggagcgg
      241 tggaacaccg cttcggcaac gagcttccgg cgtctccagt gaagtgactg acggataatg
      301 gttcatgcta ccgggctaat gaaacacgcc agttcacccg gatgttggga ctttgttgaa
      361 ccgaagaaca cggcggtgcg gagtccggag agtaacagaa tagcaaagag cttcgtgaaa
      421 acaataaagt gtgactacat cagtatcatg cccaaacctg acgggttaac ggcagcaaag
      481 aaccttgcag aggcattcga gcattataac gaatggcatc cgcatagtgc gctggattat
      541 cgctcgccac gggaatatct gcggcagcgg gccaatgata acaggtgtct ggaaatatag
      601 gggcaaagcc acccacgaac gatccagcat ataatcaaga aattagattt catttaacat
      661 aagatagatt atgtgcacca agaggcattt ccttaatttc tgaatttaca ggctcttttg
      721 ctctctttgc tatgctgtgg atttggaaag atagagtgct ggctgattat ttaggtattt
      781 tcatcagcat gtccctgata gcactgttcc atcagcgctt tcaggcgata atagtagacc
      841 ttgggtagcc tctgctcatg tgtaacttga taccccgaga acagccacga tgatctaagc
      901 ggttcttatt tctccggtct cagggtcggt aatgccaaca gtaagtccac agtaatcaac
      961 gaagagtttt ttgaaccgcc ccggaaatcc tggagactaa acgccctgag aaagaggtaa
     1021 acagaatgac taaaaataca cgtttttccc ccgaagtccg tcaacgggca gttcgtatgg
     1081 ttctggaaag tcagggcgaa tatgactcac aatgggcggc aatttttagc catgaaaacg
     1141 taatcaactc ggtgaaccaa ttcaaaaaat atactttata cctgcggaag tgagaaaacc
     1201 aatatattca atacttgcac tatttttaat ggtgccagat atattggtac tttgctcata
     1261 aagagatgta tcaccttttt tattagaaat gtaatttctg gagccttcta tataaaattt
     1321 tgcactgggg gtgacatagt atccaatatt cgcagcaaca gagcagtaat tttggttttt
     1381 tatttcatct ttaaaaattg tctcagtctg atagtgttta tctgtatcag atgaacgaac
     1441 ccaaccacta tatttcagtt ctgctccaaa ctcaaaattc tcatggcggt aattagcagt
     1501 taatccaata taaggtattt tgaagagctg tttgtaacct attgttcttt caccactagg
     1561 atgtgtccct tttttattcc tgttcccccc gttttcacta taaatataac tccctcccat
     1621 tgcattaaaa ctgtaacgac tctcttggta acctgctatt agtccaagtc gataatccaa
     1681 attatttaat aaccaacctt taagattcag ctcgaattcg ttagcatcac ggagatgcgt
     1741 attgggatgc cagctctggt cggtccatac ctgaggggta ttggaattgt tccagtcttt
     1801 atcaaccatg cttgctttct ggttacctaa agtagtccaa ccggaaactc cgaaagacac
     1861 ttttggaatt agcttccaat cgatgccacc tctaacaata gtcgcattac tgtatttcca
     1921 gtccagttga ctaatttttc tccccccttc cttgggatga taaacgcgtt cttttgtttt
     1981 gccactcaga gatcctagac taatatccgt actgatgtta tccggtatga aaagtggata
     2041 gttagtggta gcatgtgtag taaagatcgc aggtacacaa agtgcaagta caaggaattt
     2101 taatttcatc gagaactcta cttttttggt tgaaatgtcc atgatcttct tactttataa
     2161 gataaacgtc aatgccaagc attattttaa ttaaatattt gtttatgtta ccaagaacat
     2221 atcttttatt ataatattta ttgaactttt gtaaaaataa aaaaatacct cattgtgcga
     2281 ataaagtaac gggggccata gatttctcta cctacctttt ggtgttcata cctgaggctg
     2341 atagtttcag gattccgcga caattctact caccttcttg ttctctcgcc aatatgaaat
     2401 caatcagaaa agcgttccat aagaacaacg ataagacaat aaattgtcat cacggctgca
     2461 atagccaacg tgcctttcag taccaacatg acaacctcct gtatttgacg taacgtgtgc
     2521 tgataaatat gtatttgtat ctcaactgac tcaatacaac acttaagtat agtttttcag
     2581 attttgtttt tcccgtgaaa tattatggaa atggcagaag caatacattg aattccgatt
     2641 cgagtgaata cggctagcgg gcacctcact ttagcactga agccatcaac ttttatttga
     2701 cttatttcaa tggaaagctt tctcactatc agtacaggta attcccatcc ttttaaagtc
     2761 gagagtcaga ctgcgactaa tacagttaaa gtgaccaaac tcagttatcc tctacacatt
     2821 ttacctcatg tttacttgga caattcattg gtgcttttat aaaagaataa atattctact
     2881 aatttaatgc atcacctaaa atagtatttt ttataataga actgtatcta tagtatatta
     2941 ttaaagctta attatttatt atgctttaat gtggttgtcc gattaaggac taatccttga
     3001 aaacgccgta tagggcggga gttcactcta ttgcgataac gtaaccgtac aatattgtgc
     3061 gtttcccaaa ttgaccacta ctgaactatt ctgggattta aatgcaataa aatggttggt
     3121 tgaaggtcgt ggtgatgatg ttagtccttt tgagaatatt ccagtgccag atcttactta
     3181 taataaagag gcttggcagt ttggataagc tattgtcctt attccactaa tacaaacttt
     3241 ttgtaaagta tattaaatat acttcatttc atactgaaat ccccggaagt attcaatcaa
     3301 acaattacac cataaaagga agagggagcc ccaattaagt tatcaagcat gtttatgata
     3361 cctgcagggt ataaattttc tggctgcaag cagacacata taaaacgtct ttattgatcc
     3421 tacccacata atatggacgc agtcgtaagt gaggctctgt gagcctaccc acgtaatatg
     3481 gacacaggcc taagcgaggt tctggttttc aaattgttcc ggactgaggc caccacacca
     3541 acggtgccgt cgccagcgat tgtaatcgca ctcgatataa ttaaacactg ttgtccgcac
     3601 tatttcccga tagataaagt gttctccgtg gagacattcc acttaggaac aattatattg
     3661 ttcatggtaa tgtttatatt tgagtataga gattaaaatg attagtccca tcaagaatat
     3721 taagaacgta tttccaataa atactgcaaa tactgaatat atagtgagaa atatttatcc
     3781 tagagttgaa catggttact ttaatgaatc accaaacata tatgacaaga agtatatctc
     3841 tggtataacc agaagtatgg cacaattaaa aatagaggag tttattaatg agaaatccag
     3901 acgacttaat tatatgaaga caatgtattc tccatgtccg gaagattttc agcctatttc
     3961 cagagacgaa gccagtatac cggaaggaag ctggttgaca gttatatctg gaaaacgtcc
     4021 tatggggcaa ttttctgtgg atagtttgta ccatcctgac ttacatgcat tatgtgagct
     4081 cccagagatt agttgtaaga tcttccctaa agaaaatagt gattttttgt atataattgt
     4141 tgtattcaga aatgattctc cccaaggaga actacgggca aatcggttta tagaactata
     4201 tgatataaaa agagagatta tgcaagtgtt aagagatgag tcgccagagt taaagtctat
     4261 taaatctgag attattattg ctcgtgaaat gggagaactt tttagttatg catcagagga
     4321 aatagatagc tacataaaac aaatgaatga caggttttct caaatcaagg ctcgaatgtc
     4381 tgttacttaa tgtatattaa tt
//
LOCUS       DAJTMC010000234         4380 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.234, whole genome shotgun sequence.
ACCESSION   DAJTMC010000234 DAJTMC010000000
VERSION     DAJTMC010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4380
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.234"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            <1..349
                     /locus_tag="OO130_003935"
     CDS             <1..349
                     /locus_tag="OO130_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5367147.1"
                     /translation="EGGEGHLSSFFVQTDKASINVAYHHTVNQQKELGTFGAFDHDWH
                     TLAFKFKGSNSINVTPVLDGVDGQAFDLVKWANTANGLNRFVITDITGSAETYPVLID
                     TVEVKANKAGAAA"
     gene            489..671
                     /locus_tag="OO130_003936"
     CDS             489..671
                     /locus_tag="OO130_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1378 family protein"
                     /protein_id="HCR5367148.1"
                     /translation="MTFIHQVMLYFCTAVCVLYLLSGGYRAVRDFWCRQIDKRAAEKI
                     SASQSAGSKPEEPVTP"
     gene            595..942
                     /locus_tag="OO130_003937"
     CDS             595..942
                     /locus_tag="OO130_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HCR5367149.1"
                     /translation="MIKGPLRKSAPVSQPEANPKNPLLLNNPFQRENPMSEITSLVTA
                     EAVKEVLRSEEVRSALKQKLRQNLESRLDAEVDAILDELLGVAAVPEPEGIAGDGSAS
                     DGGEPTPDSDMMM"
     gene            1019..1234
                     /locus_tag="OO130_003938"
     CDS             1019..1234
                     /locus_tag="OO130_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HCR5367150.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRFSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
     gene            1239..1583
                     /locus_tag="OO130_003939"
     CDS             1239..1583
                     /locus_tag="OO130_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HCR5367151.1"
                     /translation="MKKKYELVVKGINNYPNKITVTVALEIGGYPSLLLPDVAISLDR
                     TEDATLEFYEAEAKKQAKQFFMDVAAGLCEGDGPLPEKRPVILEAQDVLITYRGKLPG
                     IITGSLKTPPLA"
     gene            1634..2167
                     /locus_tag="OO130_003940"
     CDS             1634..2167
                     /locus_tag="OO130_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HCR5367152.1"
                     /translation="MNTKIKYGLSAAVLALIAAGAPAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGAILVDSKPVVPGMKLSKEKCDQVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYRRINAGDRRGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWDIDR"
     gene            2323..2505
                     /locus_tag="OO130_003941"
     CDS             2323..2505
                     /locus_tag="OO130_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310767.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5367153.1"
                     /translation="MAKYKRILLRLTMKNGLELKAPVTDDISRALAFAIKWVAVGVAV
                     SPMLYGLAKLVIALKS"
     gene            2832..2984
                     /locus_tag="OO130_003942"
     CDS             2832..2984
                     /locus_tag="OO130_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001228697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HCR5367154.1"
                     /translation="MLPLVVSACGSTPPAPVPCVKPPAPPEWIMQPAPDWQTPLNGII
                     SPSESG"
     gene            3070..3294
                     /locus_tag="OO130_003943"
     CDS             3070..3294
                     /locus_tag="OO130_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5367155.1"
                     /translation="MKKLLVTVKPFQGTIPFRILQRGRVLVEGSFSGKCTQLHSRTFQ
                     VNATNEELTVECTMNAAKCRMVSAALQPVC"
     gene            3639..3965
                     /locus_tag="OO130_003944"
     CDS             3639..3965
                     /locus_tag="OO130_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HCR5367156.1"
                     /translation="MKRNLPLIILLSSLVMGCTQHKTDMPRQLVKALPQYPAYAAANY
                     IKGRVDVKFDIGADGTVTRIEFIRSEPHHLFDEQVVKAMAKWRFEKDRPCKGVKKTFI
                     FSPSAP"
     gene            complement(4097..4297)
                     /locus_tag="OO130_003945"
     CDS             complement(4097..4297)
                     /locus_tag="OO130_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HCR5367157.1"
                     /translation="MSSKNRTRRTTTRNIRFPNQMIEQINIALDLKGSGNFSAWVIEA
                     CRRRLATDATHLRPASMKNNEK"
BASE COUNT         1120 a          935 c         1203 g         1122 t
ORIGIN      
        1 tgagggtggt gaaggccatc tgagttcttt ctttgtccag acagataaag ccagtattaa
       61 tgttgcatac catcatacag ttaatcaaca aaaagaactt ggcacgtttg gcgcattcga
      121 ccatgactgg catacgcttg catttaaatt taagggcagt aacagcatta atgttactcc
      181 ggtgcttgat ggtgtggatg gacaggcgtt tgacctggtg aaatgggcca atactgctaa
      241 tggactcaac aggtttgtca ttacggatat tacaggtagt gcagaaacct accctgtact
      301 tattgatacg gtggaagtta aagcaaacaa agctggagca gccgcataat tgctaaaaaa
      361 agccgccagc ggcaggaatg gaagctggcg gaggtaatcc caatggagaa tgtaaagaaa
      421 agatgctttc gtacattggt tttttaaatg aaaacaattc tcattgtcaa ccataacggt
      481 aagaaattat gacatttatt catcaggtga tgctgtactt ctgtacggcg gtctgtgtgc
      541 tgtatcttct ttcgggtggg tacagggctg tgcgcgattt ctggtgcagg cagattgata
      601 aaagggccgc tgagaaaatc agcgccagtc agtcagccgg aagcaaaccc gaagaacccg
      661 ttactcctta acaacccctt tcagcgagaa aatcccatgt cagaaatcac atccctggtc
      721 actgcagagg cagtgaagga agtcctgcgc tctgaagaag tccggagcgc actgaaacag
      781 aaacttcgcc agaatcttga gtcgcgtctt gatgcagaag tggatgccat tctggatgag
      841 ctgctgggcg tagcagcggt tcctgagccg gaaggtatcg cgggtgacgg gagtgcttca
      901 gatggcggtg aacctacacc tgacagcgac atgatgatgt aagcatgcgc aagggaccat
      961 cggtgtgtgc cggtggtctt tatattgttg tgagcttccg gattgcggga gacggggtat
     1021 gtaccagatg gaaaaaatca caacaggtgt gtcatacacc acgtcagcgg tgggaacggg
     1081 ctactggttc ctgcagttgc tggacaggtt ttccccgtct cagtgggcgg caataggcgt
     1141 gctggggagt ctgttgtttg ggctgctgac atatctgact aacctgtatt tcaaaatcag
     1201 agaggaccgt cgtaaggcgg cgcggggaga gtaaagcgat gaagaaaaaa tacgaactgg
     1261 ttgttaaagg gataaataat tacccgaata agattactgt tactgtggca ctggaaattg
     1321 gtgggtatcc gtcactgttg ttgccagatg tggcgattag tcttgaccgt actgaagatg
     1381 ccacgctgga gttttacgaa gctgaggcga aaaagcaggc gaagcagttt ttcatggatg
     1441 ttgctgccgg gttatgtgaa ggggatggtc cgttgccgga aaagcgcccc gtaattttag
     1501 aggcgcagga tgtgttgata acctacagag gaaaactacc gggaataatt actggttctc
     1561 tgaagactcc accgctggcc tgaagactta acatatccag ggatttgaaa tcgataaacc
     1621 ctgataaata tccatgaaca ccaaaatcaa atacggcctg tcggctgccg ttctggcgct
     1681 gattgccgca ggtgcgcctg cgcctgaaat cctcgaccag tttctggatg aaaaggaagg
     1741 taaccacacc acggcatacc gtgatggcgc gggtatctgg accatctgcc gcggtgccat
     1801 cctggtggat agtaaacctg tcgtcccggg catgaagttg tcgaaggaaa aatgcgacca
     1861 ggtcaacgcc attgagcgtg ataaggcgct ggcatgggtg gagcgcaata ttaaagtgcc
     1921 gctgaccgaa ccccagaaag cgggtatcgc gtcattttgt ccctataaca ttggccccgg
     1981 taagtgtttc ccgtcgacgt tttacagacg gattaatgct ggtgaccgca ggggagcatg
     2041 cgaagcgatt cgctggtgga ttaaggacgg tggcagagac tgccgtatcc gctcaaataa
     2101 ttgctacggt caggtatccc ggcgagacca ggaaagtgcg ctggcgtgct gggacatcga
     2161 cagataagca gaatattttg ctgaaaataa ggcatgagca cgcggacgga taacacgaaa
     2221 tcctgcgaac tggcgaaacg taagtgaata aaagtaaaaa ccccgtttgt tggcaccagg
     2281 cggggttttg tgtttctgac cttgagtaag gcaagggaga acatggcgaa gtataaacga
     2341 attctgttga ggttgaccat gaaaaacggc cttgaactga aagcgcctgt aactgatgac
     2401 atcagcagag cactggcttt tgccattaag tgggtggcgg tcggtgttgc tgtgtccccg
     2461 atgctgtatg ggctggcaaa actggtcatt gcgttgaaat cgtgaaggga ggattaagca
     2521 tgtcagacaa actcataacg ctggcgaaga tcctctgtgt aattgtcggc atttcatttt
     2581 cactaatgct ggttgctctt tttctttcca tggcctggat gatgttgtct tcgtcggggc
     2641 tgctggggtg aacataaacc gaatgctttc cgcgtttacc gttattctgc tggtggtctg
     2701 tggtgcgctg tggctggcaa cagaccatta ccgtgataac gccatcacct acaaagcgca
     2761 gcgcgataaa aaagccagag agctggagct ggcaaacgca accattactg acatgcagca
     2821 gcgccagcgt gatgttgccg ctggtcgtaa gcgcctgcgg atcaacgcca cctgctccgg
     2881 taccgtgcgt gaagccaccg gcacctccgg aatggataat gcaaccggcc cccgactggc
     2941 agacaccgct gaacgggatt atttcaccct cagagagcgg ttgatgacaa tgcagaagca
     3001 actggaaggg gcgcaggaat atatccgcac tcagtgtatt aactagtatt tttgttatcc
     3061 ggagaatgca tgaagaaatt actggtaacc gtaaagcctt ttcagggaac aattccgttc
     3121 cgtattttgc agcgtggtcg tgttcttgtt gaaggttcgt tcagtggtaa atgtacgcaa
     3181 ttacactccc ggacctttca ggtgaatgcc acgaatgaag agctaaccgt tgagtgtacg
     3241 atgaatgccg ctaaatgccg catggtatcc gctgcattac agccagtgtg ttgagcgacc
     3301 ttattaacca tgcgcggtat tgtcgccgta tccccgcatt aacagagacc gcagcccgac
     3361 cgggagactc ctctgcgcga gtgtgcgggg ataatcaaaa acgatacaca ccggggttta
     3421 ccgcgttaac ggagcgcggc gttgtcccct catggtcgct ggtccggtgc gatggtggaa
     3481 gaaaccggac gatgtgttac ctcgcaagct ctgttatgtc atgtgtctga tttgtgattt
     3541 aagtcggata attgtcgttg ccattaagca gaggattgat gaccgacagg gtggcattgt
     3601 tagaataaga cttattctta tctgtgccgg gaatgaaaat gaaaagaaac cttccgttaa
     3661 ttattttgtt gtcttctctg gttatgggct gtacgcaaca taaaacagat atgccccgac
     3721 agttggttaa ggcattacca caatatccgg cctatgcagc ggcaaattat ataaagggac
     3781 gggttgatgt gaagtttgat attggtgctg atggtactgt cacccgaatt gagtttatcc
     3841 gttcagagcc gcaccatttg tttgatgagc aggttgtaaa ggcgatggca aaatggcgat
     3901 ttgagaagga caggccgtgt aaaggcgtga agaaaacgtt tatctttagt ccttctgcac
     3961 cctgattatt tcatcagaaa ttaattatca ctctgttgtt attctgtaca tcccggcagg
     4021 gtaagtcttg ttccgtcgga tatgaagatg aaatattgtt ggaggacagt gggtacctgc
     4081 tcctgtaacc gaacgttcat ttctcgttat ttttcatgct ggccgggcgc agatgcgttg
     4141 catctgttgc cagccttctc ctgcaggctt caataaccca cgctgaaaag tttcctgaac
     4201 ctttcagatc aagagcgatg ttaatttgtt caatcatctg gtttggaaat cggatgttgc
     4261 gggttgttgt tctgcgggtt ctgttctttg atgacataat gtttccccat attcagtgtt
     4321 gctgatttgt attatctgaa gttgctttta cgttaatttg atgcagatca atcaatacga
//
LOCUS       DAJTMC010000235         4319 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.235, whole genome shotgun sequence.
ACCESSION   DAJTMC010000235 DAJTMC010000000
VERSION     DAJTMC010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4319
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.235"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(<1..975)
                     /locus_tag="OO130_003946"
     CDS             complement(<1..975)
                     /locus_tag="OO130_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 family protein"
                     /protein_id="HCR5367158.1"
                     /translation="MIRKSATGVIVALAVIWGGGIWYTGTQIQPGVEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSHFQMQISFDNGAPDLNIKPGQKVAFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLTRLE
                     EKKFPVGESESKFNQVNIINQGEDVAQIDAFVAKTRLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEV"
     gene            complement(1130..1756)
                     /gene="dsbA"
                     /locus_tag="OO130_003947"
     CDS             complement(1130..1756)
                     /gene="dsbA"
                     /locus_tag="OO130_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HCR5367159.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
     gene            complement(1773..2759)
                     /gene="srkA"
                     /locus_tag="OO130_003948"
     CDS             complement(1773..2759)
                     /gene="srkA"
                     /locus_tag="OO130_003948"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HCR5367160.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEDRRRIVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDSTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(2836..3105)
                     /locus_tag="OO130_003949"
     CDS             complement(2836..3105)
                     /locus_tag="OO130_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HCR5367161.1"
                     /translation="MKCKRLNEVIEFLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            3175..3759
                     /gene="mobA"
                     /locus_tag="OO130_003950"
     CDS             3175..3759
                     /gene="mobA"
                     /locus_tag="OO130_003950"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HCR5367162.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQASGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPPDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            3741..4268
                     /gene="mobB"
                     /locus_tag="OO130_003951"
     CDS             3741..4268
                     /gene="mobB"
                     /locus_tag="OO130_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HCR5367163.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLQFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGARHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
BASE COUNT         1169 a         1020 c          966 g         1164 t
ORIGIN      
        1 aacttcatcg tttgctaact cagggtgtgc agcaagctgc ttctgcatcg caatgttata
       61 ctggatgata aattggcgca ctgctgaggg atcaatagat tcagcaatca atgaccactc
      121 accactaccg agttgctgat tcccttttgt taacttatca agttcgtagg tcagattgac
      181 attgatataa tctttgtctt taacgcgatc cagcctggtt tttgcaacga aagcatcgat
      241 ttgggcaacg tcttccccct gattgataat attaacctga ttaaatttcg actcgctttc
      301 accaaccgga aatttctttt cttctaatcg tgtcagagaa tcaatggtaa aactttttgc
      361 cgttactttg ttcattggtg ataattgcag aacaatatct tcgactttgc cttcaatatc
      421 caggttagac aatgaactac catcaccatt aaaagtaaac tggccctcac caaaagaaaa
      481 ctttccatac tctgcagggg caacatctaa tgtcgtagag aatgagccac caaacgcgaa
      541 tcgcaatgtc gcttccacgg gcgatttatt tttcgcggcg ataaatagcg gttgtgtcag
      601 ttcattattc actaagttca cttttgccgc tgccagtgct gggatgacat taccatgcat
      661 taacattgtg atgggcaacg gaccgtgctc aacatccaca tcaaatgcaa ctttctggcc
      721 tggcttgata ttgagatcgg gtgcaccgtt atcaaatgaa atttgcattt gaaaatggga
      781 attaaaaaag cctttgtcaa agtttttata acttaacgtc atatcgtagg catgttcacc
      841 tttcttttta gcatcgttaa aatctttaat aaatttttcg acaccaggct gaatttgcgt
      901 acctgtgtac catatgccac caccccagat tacggctaac gcaacaataa cacctgtagc
      961 tgacttacgt atcatataaa tattccctga taaaacagca aaactttgaa tatccactta
     1021 tgctgaaata atgtccagcg gcaggatgta ttatcagaat gtatttatat ttattaaata
     1081 ataaaaaaag cccgtgaata ttcacgggct ttatgtaatt tacattgaat tattttttct
     1141 cggacagata tttcactgta tcagcatact gctgaacaaa aacatccata ttgctggtat
     1201 ccataccctg cggattcagc tgatatttac cgttaacaaa catcgccgga acaccgcgca
     1261 attgcacgtc agctgcagct ttttcctgct gagctaccag agatttcacc acgaagctgt
     1321 tccacgccgc gtcgtactct tcacctttaa tacctgcgtt gataaataca tcgcggatat
     1381 cagaagcaga acgaatggtc tgggttttct gtacgccttc aaacagcgga acagtcactt
     1441 tgtcttccac gcccagcgcc atcgccacag cccatgcctg agtcagatct ttgcccaggt
     1501 caccacccat gaagttgacg tggtatttag tcatcttcac gccttccggc agttttttct
     1561 tcacattatc agaaatatgc agaacttctt caaactgata gcagtgcgga cagaagaaag
     1621 agaaaaactc cagcacttgc ggcgcgccag ctaccggttt ttccagggta gtgtactgtt
     1681 taccatcttc atactgcgcc gccgatgcgc taaacgctaa aactaaacca gccagcgcca
     1741 gccaaatctt tttcatgatc tactctctcc gattaataca taggtgttaa ttgtaaaggg
     1801 ggttcttgta gaacttttgc ctgttctata aaagtcgccg tttgtcgcag ccagtaatct
     1861 tccccggtta accacgggaa atttttcggg aacgcgggat cagcccaacg ccgcattagc
     1921 caggcaagat aataaaccaa acgcatggcg cgtaaaggtt caatcagtcc gatttcagcg
     1981 gtgtcgaact cgctaaattc ttcataagct tcaataatag tttccagttg catccgctgc
     2041 tcggctttat cgccattgag caacatccac aaatcctgaa tagctggacc attacgtgca
     2101 tcatccagat caacaaacat tggaccatcg cgccagagaa tattcccggc gtggcagtct
     2161 ccatgtaagc gcagaacggt ggaatcttcc cgccagtgcg ctgtaacggc ggcgatcagc
     2221 tcatctgtcg ctttcaggaa tgccgctttc aacccggaag gtatcagtgt agcgtcctca
     2281 aacagcttgc gtggctcaat gagatattca ttcagaccga tggtcgggcg atggataaaa
     2341 agctgtttgc gccccgtctg gtgcatacgc cctaaataac gcccaaccgc ttccatctga
     2401 tcgatattat cagcttcgaa ctggcgacca ccgacgcttg gaaaaacagc gaaataaaat
     2461 ccctgatgat tcaataaagt ctgaccgtta aaggccacag gtgctgcgac cggaacttca
     2521 tcatttacca actgcaacgc aaattgatgt tcttcgagga tttgatcggc tgtccaacgt
     2581 tcagggcgat aaaatttgac gacaatacgt cgacgatctt cgtcctgaaa ttgatagaca
     2641 cggttttcat agctgttaag cggggtaaga ccggaatcca cccggatccc ttgctcaaat
     2701 agagcatcca tgatggtatc cgggtgtagt gtctggaaag taaaagcgct gttgttcatc
     2761 ccatcatccg gaaaacatta cgaatgatga aggatatcat ttcacgatga ttttggtggc
     2821 gccgcttaca agcttttact ctttaattac gccgcgagcg cgtaacagcg ccgttttgaa
     2881 gtcttcttca taatctttct gcaaacccgg gatcaccgca tctttcgcgg aatcacgcat
     2941 tttcaggtga tagatcagaa tgtcatccgt caaatccgcc agttcgccgt caaaacctga
     3001 ctctttcgcc agtttctgca aaaattgcaa caggttaagg tctggctctt tttgccaggc
     3061 tggctggagg aattcaataa cttcattcag acgtttacat ttcatagtga tgctccttac
     3121 tcttgagaca gacacgttag cagggtcaat cccacaataa aagaggcgat atcggtgaat
     3181 ctgatgacga cgataacagg cgttgtgctg gcaggcggta aagccagacg aatgggtggc
     3241 gtagataaag gattgcttga attaaacggc aaaccattat ggcaacatgt cgctgacgcg
     3301 cttatgacgc agctctctca cgtcgtggtt aatgccaatc gtcatcagga aatctatcag
     3361 gcaagcggtc tgaaagtgat tgaagattca ctggcggatt acccaggccc tctggcagga
     3421 atgctttcag taatgcagca ggaagctggt gagtggtttt tgttttgccc gtgcgatacg
     3481 ccttacattc cccctgattt agcagcccgg cttaatcatc agcgcaaaga tgcgcctgtc
     3541 gtgtgggtcc atgacggtga acgcgatcac ccgactattg ctctggtaaa ccgcgctatt
     3601 gagcctttat tactggaata tctgcaagca ggagaacgcc gggtaatggc atttatgcgt
     3661 ctggctggcg gtcatgcggt tgatttcagc gatcataaag atgcatttgt taacgtgaat
     3721 acgccagagg agcttgcccg atggcaggaa aaacgatgat accgttactc gcctttgccg
     3781 cgtggagtgg taccggaaaa actacgcttt tgaaaaaact gatcccggca ttatgcgcca
     3841 gagggatccg tccagggctg attaagcata cgcaccatga tatggatgtt gataagccag
     3901 gcaaagatag ctatgagctg cgcaaggctg gcgcggcaca aaccatcgtt gccagccagc
     3961 aacgatgggc cttgatgacg gaaacaccag acgaagaaga gctggatcta cagtttctcg
     4021 caagtcgaat ggatacctca aagctggatt tgattctggt cgaagggttt aagcatgaag
     4081 agatcgcaaa gattgtgctg tttcgcgatg gagccagaca tcgacctgaa gaattagtga
     4141 tagacaggca tgttattgct gtagccagtg atgtgccgct taatcttgat gtcgcattac
     4201 tggatattaa tgatgttgag gggctggctg attttgtggt ggagtggatg caaaagcaga
     4261 acggataagc aagagtaggc tggatcagac gcagccagtg tcgcatccga cctttttct
//
LOCUS       DAJTMC010000236         4296 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.236, whole genome shotgun sequence.
ACCESSION   DAJTMC010000236 DAJTMC010000000
VERSION     DAJTMC010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4296
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.236"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            49..2211
                     /gene="ybiO"
                     /locus_tag="OO130_003952"
     CDS             49..2211
                     /gene="ybiO"
                     /locus_tag="OO130_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HCR5367164.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALTHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGLWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIHDETARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLIIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLVGVRAPVQTY"
     gene            complement(2271..3197)
                     /gene="rlmF"
                     /locus_tag="OO130_003953"
     CDS             complement(2271..3197)
                     /gene="rlmF"
                     /locus_tag="OO130_003953"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HCR5367165.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVAFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            3473..3732
                     /locus_tag="OO130_003954"
                     /pseudo
     CDS             3473..3732
                     /locus_tag="OO130_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
     gene            3997..>4296
                     /locus_tag="OO130_003955"
     CDS             3997..>4296
                     /locus_tag="OO130_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HCR5367166.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQTNADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPG"
BASE COUNT          998 a         1133 c         1076 g         1089 t
ORIGIN      
        1 ccagcatcta tacttatctt tttgctctgt tttctcactg gaggagtcat gcggtggatc
       61 ctgttcatcc tcttctgcct gctgggcgca cctgcccacg cggtatccat acccggcgtt
      121 acaaccacaa cgacaacgga ctcaacgact gaaccggccc cggaaccgga tatcgaacaa
      181 aaaaaagcgg cctatggcgc actggcggat gtgctggata acgacacctc gcgtaaagaa
      241 ttgatcgacc agttgcgtac tgtcgccgcg acgccccctg cggaaccggt cccgaagatc
      301 gtgccgccga cgctggttga agagcaaacc gtgctgcaaa aggtcaccga agtcagccgc
      361 cattatggtg aagccctttc cgcccgcttc gggcaacttt atcgcaatat taccggctcc
      421 ccgcataagc cgtttaatcc acaaacattc agcaatgcgc tgacccattt ttcaatgtta
      481 gcggtattag tgtttggttt ttactggctg attcgcctgt gcgcactgcc gctgtatcgc
      541 aaaatgggcc tgtgggcgcg gcaaaaaaat cgtgagcgca gtaactggtt gcagcttccg
      601 gcgatgatta tcggggcgtt tattatcgac ctactgttac tggcactgac attatttgtc
      661 ggccaggtat taagcgacaa cctgaatgca ggcagtcgca ccatcgcttt ccaacaaagt
      721 ttgtttctca acgcctttgc cctgattgaa tttttcaaag ctgtactacg cctgattttt
      781 tgcccaaacg tggctgaact gcgcccgttc acgattcatg acgagaccgc ccgttactgg
      841 agtcgtcgcc tgagctggtt aagcagtttg ataggttatg gcctgattgt ggccgtgccg
      901 attatctcta atcaggtgaa tgtacagata ggtgcgctgg cgaacgtcat cattatgctg
      961 tgcatgaccg tctgggcgtt gtacctgatc tttcgcaata aaaaagagat tacccaacat
     1021 ttgctcaact ttgcggagca ttcgctggcc tttttcagcc tgtttatccg cgcctttgcg
     1081 ctggtgtggc actggctggc aagcgcctat tttatcgtgc tgtttttctt ttcgttgttc
     1141 gatccgggca acagcctgaa atttatgatg ggtgcaacgg tgcgcagcct ggcgattatt
     1201 ggtatcgcag cgtttgtttc cggtatgttt tcccgctggc tggcgaaaac catcaccctc
     1261 tcgccacata ctcagcgtaa ctatccggag ttgcaaaaac ggctgaatgg ctggctttcg
     1321 gcggcgctga aaacggcgcg tattctgacc gtctgcgtgg cggtaatgtt gctgttgagc
     1381 gcatggggat tgttcgattt ctggaactgg ctgcaaaacg gcgcggggca gaaaaccgta
     1441 gatatcctga tccgtatcgc acttattctt tttttctcgg cggttggctg gacggtgctc
     1501 gccagtttga tcgaaaaccg gctggcttcg gatattcatg gccgcccgct acccagcgcc
     1561 cgtacgcgta ccctgctgac gctgtttcgt aacgcgctgg cggtgattat cagtaccatc
     1621 accatcatga ttgtgttgtc ggaaatcggc gtcaatatcg cgccattgct ggcaggtgcc
     1681 ggggcattag gtctggctat ctcgtttggt tcgcaaacgc tggtgaaaga tatcattacc
     1741 ggggtgttta ttcagtttga aaacggcatg aacactggag atttggtgac tatcgggccg
     1801 ttgaccggca cagtggaacg gatgtcgatt cgctccgtgg gcgtgcgcca ggataccggg
     1861 gcgtatcaca tcattccgtg gtcttcgata accacctttg ctaacttcgt ccgcggtatt
     1921 ggttcggtag tggcaaacta cgatgttgat cgccatgaag atgctgataa agccaatcag
     1981 gcactgaaag atgcggtagc ggaattgatg gaaaacgaag aaattcgcgg gctgattatt
     2041 ggtgaaccga attttgccgg gattgtcggc ttaagcaata ccgcgtttac actgcgtgtt
     2101 tcgttcacca cgctgccact caaacagtgg acggtacgct ttgccctcga cagccaggtg
     2161 aaaaaacatt tcgacctggt gggcgttcgc gcgccagtgc agacttatta ggtgctgcct
     2221 actccgggcg cgaccccggc tgaaccgtta ccgccggggg agccaacgct ttaacgctgg
     2281 cgattgacaa aacggcggcg ctgctcgtcg ttcataaagg tccaggcaat aaagcgactc
     2341 tgcttttgcc cctgggccat ctcttttttt accaccttca ccgcgcccac gtcggtcaga
     2401 gcacgataca acggcggtaa gttttcacca cgagatacca gcgaggtaaa ccacatcacc
     2461 tgcttcgcga agcctttgct ctcttcaatc atctttttga taaaggcgac ttcaccacct
     2521 tcacaccaca actcctgttg ctgaccacca aagttcagtg catcgtcttt gttcagcccc
     2581 aggttacggc gtttacgctc actacctgcc cgcgctgcag cggcggaatc gtggaatggc
     2641 gggttacaca aggtcgcgtc gtattgctcg tttttatgga tgatgccgtt aaaaattgcc
     2701 ccactctctt tttgccgacg cagacgaatg gcgcggttaa gccccggatt agcactgata
     2761 atcgcctgcg cactgcttaa cgcctggctg ctggtttcac taccggtaaa acgccagcca
     2821 tattcatgta cgccaatcag cggataaata cagttcgcac caacgccgat atccagaatg
     2881 ctggcattcg ctggaattgt tccgcttgct tcagccagta aatcggcaag gtgatgaata
     2941 taatccgccc ggccgggtac tggtgggcag agaaaaccgt cggggatatc ccagttcgct
     3001 acggcgtaaa aatgggccag caacgcctta ttgagcgcct tcaccgccag cggattggca
     3061 aagtctacgc tttgctcccc ggcgggtgta agcgtgagga attgcctgag ttcaggattg
     3121 acctgacaaa gcgtggcgag atcgtagcgg ctgtgatgac ggttgcgcgg atgcaacccc
     3181 ggtttctggg cggacatagc attctccttt aagcagcggc gtaagatacc cgcataacgc
     3241 gacagggtaa atatctggct gcgtttatca ttccccaaaa ccttaacatg gctatggttt
     3301 atggcaattc atcataaaaa tcagcattat gcatgtcatt gatttgtctt attttgtaaa
     3361 aatatatcgt tcaaaaaata agcacatctc tgtcaatttt ttgcgtcatt tgcatcgcgc
     3421 aggcgaccta cacttatcag gcactacccg ttgtatttat gaggatgtca ttatgaaaaa
     3481 gtgcctcaca ctactgattg ccaccgtcct gagcagaatc tctctcacgg cttatgccgc
     3541 gcaaccgatg agtaacctgg acagcggtca gctacggccc gcggtaccgt ttcggcaact
     3601 ggcgcatcaa acctaagcga tctggaggat aaactggcag aaaaagcgcg cgaacaaggc
     3661 gcgaaaggtt atgtcattaa ttccgcaggc ggaaatgacc agatgttcgg tactgcaacc
     3721 atctacaaat aaccccacca acgtaactgc attcgcctct ggatgcagtt ttcgcctgtt
     3781 ttgtaatcaa aaaacatcaa atatgaactc aatgtaaata aatgtatttc tttttcgcgc
     3841 aatgggtgat agaaaatcgc gccaagtgat aatgcttatc aaaattatta tcacttgcac
     3901 gaacactatc acgggattaa cagtggcatc gcatccgcag agatgctttc tcgtgacagt
     3961 gaaaatttca acatataaga aaaagtcacc tgcaaaatgg aaaacaatcg caatttccct
     4021 gccagacaat ttcattcgct cacgttcttt gccggtcttt gtattggcat cacgcctgtg
     4081 gctcaggcac tcgccgccga agggcaaact aacgcggatg acacgctggt tgtcgaagca
     4141 tcaacgcctt cgctttatgc gccacaacaa tctgccgatc cgaaattctc gcgtccggta
     4201 gcggatacta cccgcacgat gacggtgatt tctgaacaag tgattaaaga tcagggcgca
     4261 accaacctta ccgacgcgct caaaaacgtc cccggc
//
LOCUS       DAJTMC010000237         4268 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.237, whole genome shotgun sequence.
ACCESSION   DAJTMC010000237 DAJTMC010000000
VERSION     DAJTMC010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4268
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.237"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(43..213)
                     /locus_tag="OO130_003956"
                     /pseudo
     CDS             complement(43..213)
                     /locus_tag="OO130_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
     gene            523..2295
                     /gene="aspS"
                     /locus_tag="OO130_003957"
     CDS             523..2295
                     /gene="aspS"
                     /locus_tag="OO130_003957"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HCR5367167.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            2413..2865
                     /gene="nudB"
                     /locus_tag="OO130_003958"
     CDS             2413..2865
                     /gene="nudB"
                     /locus_tag="OO130_003958"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HCR5367168.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDSQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            2894..3634
                     /locus_tag="OO130_003959"
     CDS             2894..3634
                     /locus_tag="OO130_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR5367169.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEIAATL"
     gene            3669..4190
                     /gene="ruvC"
                     /locus_tag="OO130_003960"
     CDS             3669..4190
                     /gene="ruvC"
                     /locus_tag="OO130_003960"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HCR5367170.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
BASE COUNT         1053 a         1059 c         1199 g          957 t
ORIGIN      
        1 atcaaccggc tgttttaatg cttcggcgta atcggcagat cagccaactc gcaccagaaa
       61 cacgggctga ccgctggcgc gaaatttcgc cgccagcttc ccggcgcgat taactacctc
      121 atcggcagta tgtggaccac cggcaaaagg taagatgcct tcttgtaaat cgatcaccac
      181 caacgcagtg gttttagcat taagttcaag catgctgact cctgaaaaaa caaaatcgtg
      241 cctcacacct taatgcgatt agccgatggc ggaacgacaa attttgttaa tttttatgag
      301 ttcgggcaac acctttatag caaactggcg tagagtctga tttcaggccc gatataaagt
      361 ggtaacgagg cgttccagcg cgggctaggc tcttacggtg cggcagaatt tccagtataa
      421 tagccgcctt ttttcattca gttgtgacat acagctaacg ctgcgacttg gtcacctgcg
      481 cagcaggggg catccgccgg cagctaagtt aagggatatc tcatgcgtac agaatattgt
      541 ggacagctcc gtttgtccca cgtggggcag caggtgactc tgtgtggttg ggtcaaccgt
      601 cgtcgtgatc ttggtagcct gatcttcatc gatatgcgcg accgcgaagg tatcgtgcag
      661 gtatttttcg atccggatcg tgcggacgcg ttaaagctgg cctctgaact gcgtaatgag
      721 ttctgcattc aggtcacggg caccgtacgt gcgcgtgacg aaaaaaatat taaccgcgat
      781 atggcgaccg gcgaaatcga agtgctggcg tcctcgctga ctatcatcaa ccgcgcagat
      841 gttctgccgc ttgactctaa ccacgtcaac accgaagaag cgcgtctgaa ataccgctac
      901 ctcgacctgc gtcgtccgga aatggctcag cgcctgaaaa cccgcgctaa aatcaccagc
      961 ctggtgcgcc gttttatgga tgaccacggc ttcctcgaca tcgaaactcc gatgctgacc
     1021 aaagccacgc cggaaggcgc gcgtgactac ctggtgcctt ctcgtgtgca caaaggtaaa
     1081 ttctacgcac tgccgcaatc cccacagttg ttcaaacagc tgctgatgat gtccggtttt
     1141 gaccgttact atcagatcgt taaatgcttc cgtgacgaag acctgcgcgc tgatcgtcag
     1201 cctgagttta ctcagatcga tgtggaaact tctttcatga ccgcgccgca agtgcgtgaa
     1261 gtgatggaag cgctggtgcg tcatttgtgg ctggaagtga agggcgtgga tctgggcgat
     1321 ttcccggtaa tgacctttgc ggaagcagaa cgccgttatg gttctgataa accggatctg
     1381 cgtaacccga tggaactgac cgacgttgcc gatctgctga aatctgttga gtttgcagta
     1441 tttgcggggc cggcgaacga tccgaaaggc cgtgttgccg ctctacgtgt tccgggtggc
     1501 gcatcgctga cccgtaagca gatcgacgaa tacggtaact tcgttaaaat ctacggcgcg
     1561 aaaggtctgg cttacatcaa agttaacgaa cgcgcgaaag gtctggaagg tatcaacagc
     1621 ccggtagcga agttccttaa tgcagaaatc atcgaagcca tcctggatcg tactgccgcg
     1681 caagatggcg atatgatttt cttcggtgcc gacaacaaga aaattgttgc cgacgcgatg
     1741 ggcgcactgc gcctgaaagt gggtaaagac cttggtctga ccgacgaaag caaatgggca
     1801 ccgctgtggg ttattgactt cccgatgttt gaagacgacg gtgaaggcgg cctgacggca
     1861 atgcaccatc cgttcacctc accgaaagac atgactgctg cagaactgaa agctgcaccg
     1921 gaaaatgcgg tggcgaacgc ttacgatatg gtcatcaatg gttacgaagt gggcggtggt
     1981 tcagtacgta tccataatgg tgatatgcag cagacggtgt ttggtattct gggtatcaac
     2041 gaagaggaac agcgcgagaa attcggcttc ctgctcgacg ccctgaaata cggtactccg
     2101 ccgcacgcag gtctggcatt cggtcttgac cgtctgacca tgctgctgac tggcaccgac
     2161 aatatccgtg acgttatcgc cttcccgaaa accacggcgg cagcgtgtct gatgactgaa
     2221 gcaccgagct ttgctaaccc gactgcactg gctgagctga gcattcaggt tgtgaagaag
     2281 gcagagaata actgatatga ctcaaataca cgaaatcatt cgcgttgcat caaggcggca
     2341 attgagtgaa ctcccatgag catagataac tatgtgaatg ggatgagcga aggcagtcaa
     2401 cgaagaggca gcgtgaagga taaagtgtat aagcgtcccg tttcgatctt agtggtcatc
     2461 tatgcacaag atacgaaacg ggtgctgatg ttgcagcggc gtgacgatcc cgatttctgg
     2521 cagtcggtaa ccggcagcgt ggaagagggt gaaaccgcgc cgcaagctgc catgcgcgaa
     2581 gtaaaggaag aggtcaccat tgatgttgtc gctgaacaac tgaccttaat tgacagtcag
     2641 cgcacggtag agtttgaaat tttttcacat ttacgtcatc gctatgcgcc gggcgtgacg
     2701 cgtaatacgg aatcatggtt ctgtcttgcg cttccgcacg agcggcagat cgttttcact
     2761 gaacatctgg cttacaagtg gcttgatgcg cctgctgcgg cggcgctcac taagtcctgg
     2821 agcaaccggc aggcgattga acagtttgta attaacgctg cctgaacagg cagggctatc
     2881 tggagatatt tttatggcag gtcatagtaa atgggccaac accagacatc gtaaagctgc
     2941 gcaggatgct aagcgcggta aaatcttcac taaaatcatt cgtgagctgg taaccgcggc
     3001 taagctgggc ggtggcgatc cggacgctaa cccgcgtctg cgtgcggcgg tggataaagc
     3061 actgtctaac aacatgaccc gtgacacact gaaccgcgca attgcacgtg gtgtgggcgg
     3121 tgatgatgat gcaaacatgg aaaccatcat ctacgaaggt tacggtcctg gcggcacggc
     3181 aatcatgatt gaatgtctgt ctgacaaccg caaccgtacc gttgctgaag tgcgtcatgc
     3241 attcagcaaa tgtggcggta acctcggtac agatggttcc gtagcctatc tgttcagcaa
     3301 aaaaggcgtg atctccttcg agaaaggcga tgaagacacc atcatggaag cagctctgga
     3361 agcaggtgct gaagacgttg tgacctatga tgacggcgcg attgatgtct acactgcatg
     3421 ggaagaaatg ggtaaagtgc gcgacgctct ggaagcggca ggtctgaaag cagacagcgc
     3481 ggaagtttcc atgatcccgt ccaccaaagc tgatatggat gcagaaaccg caccgaaact
     3541 gatgcgtctg atcgatatgc tggaagattg cgacgacgtg caggaagttt accataacgg
     3601 tgaaatctct gatgagattg cagcgactct ctgatgaggc ctgctaaaca gcaaaacgga
     3661 gacgcgtgat ggctattatt ctcggcattg acccgggttc gcgcgtgacc ggctatggcg
     3721 tcatccgcca ggtaggcagg caactgtcct acctgggtag cggatgcata cgcaccaagg
     3781 tggatgattt accgtctcgt ctgaagctca tctatgcggg cgtgacggaa atcatcaccc
     3841 agttccagcc agattatttc gccattgaac aagtctttat ggccaaaaat gccgactcgg
     3901 ctctgaaact ggggcaggcg cgcggcgtgg cgattgtggc ggcggtgaat caggaattgc
     3961 cggtatttga atatgcggca cgtcaggtaa agcaaacggt ggtaggtatt ggtagtgccg
     4021 aaaaaagtca ggtgcagcat atggtccgca ccttgctgaa actgcccgct aatccacagg
     4081 cggatgccgc cgatgcgtta gctatcgcga ttacccattg tcacgtcagc cagaatgcaa
     4141 tgcagatgag cgaatcgcgg ctgaacctgg cgagagggcg actgcgttaa gttataccgc
     4201 ctcggtcagt tcaggctgag gcgtttcaac tccctccgcg tgaatcaaca ccaaacccgg
     4261 atgatgcc
//
LOCUS       DAJTMC010000238         4183 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.238, whole genome shotgun sequence.
ACCESSION   DAJTMC010000238 DAJTMC010000000
VERSION     DAJTMC010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4183
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.238"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OO130_003961"
     CDS             complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OO130_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR5367171.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(324..399)
                     /locus_tag="OO130_003962"
     tRNA            complement(324..399)
                     /locus_tag="OO130_003962"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(364..366),aa:Thr,seq:ggt)
     gene            complement(406..480)
                     /locus_tag="OO130_003963"
     tRNA            complement(406..480)
                     /locus_tag="OO130_003963"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(445..447),aa:Gly,seq:tcc)
     gene            complement(597..681)
                     /locus_tag="OO130_003964"
     tRNA            complement(597..681)
                     /locus_tag="OO130_003964"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(645..647),aa:Tyr,seq:gta)
     gene            complement(690..765)
                     /locus_tag="OO130_003965"
     tRNA            complement(690..765)
                     /locus_tag="OO130_003965"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(730..732),aa:Thr,seq:tgt)
     gene            1127..2077
                     /gene="coaA"
                     /locus_tag="OO130_003966"
                     /gene_synonym="panK"
     CDS             1127..2077
                     /gene="coaA"
                     /locus_tag="OO130_003966"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HCR5367172.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAAQRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2106..3071)
                     /gene="birA"
                     /locus_tag="OO130_003967"
     CDS             complement(2106..3071)
                     /gene="birA"
                     /locus_tag="OO130_003967"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HCR5367173.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3068..4096)
                     /gene="murB"
                     /locus_tag="OO130_003968"
     CDS             complement(3068..4096)
                     /gene="murB"
                     /locus_tag="OO130_003968"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HCR5367174.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQYATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLLKEWQPVLTYGDLTRLDPTTVTPQQVFNVVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1079 a         1041 c          932 g         1131 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcgcgagc agcaccgccg taggttttag ccagtacggt agtgattgca
      121 gcggtcagcg ttgttttacc atggtcaacg tggccgatag taccgacgtt aacgtgcggt
      181 tttgtacgtt caaacttttc tttagacatc gattgtccct ctaagacacg gataaatcgg
      241 tgatatcacc acatcaacca ggcaacatgc ccgacttgtt gaatgcaata aacagaagga
      301 aaaaacaggg aggagaaaag aagtggtgct gataggcaga ttcgaactgc cgacctcacc
      361 cttaccaagg gtgcgctcta ccaactgagc tatatcagca catcttggag cgggcagcgg
      421 gaatcgaacc cgcatcatca gcttggaagg ctgaggtaat agccattata cgatgcccgc
      481 atcctggaac tcggctacct gattttcatt ctgcactgaa tatcgagaga agctctcttt
      541 attcgagccg gtaagcgaac ttatcgtctc gggctacgcc atcgcgtggc cgaaattggt
      601 ggtgggggaa ggattcgaac cttcgaagtc tgtgacggca gatttacagt ctgctccctt
      661 tggccgctcg ggaaccccac cggacttgat ggtgccgact accggaatcg aactggtgac
      721 ctactgatta caagtcagtt gctctaccta ctgagctaag tcggcatcaa gtagcgcgca
      781 ttctatggag acatgcgagt tcatgcaact aaaaaattgc ataatttgtt ttattggtca
      841 cattttatgc gacacgatga agaaacagcc gaatggaggg cagattagca cactttttca
      901 acatcattgt gctcaacaat gcgctcctgc taaaccataa ttcttcttat cagatggaat
      961 atctgtcaca ttgcttttca acgatagctt cctaggagag attttttctt attattcctc
     1021 cccatctggt gttaccctcc tgcccattaa cccattcaac agaactgtga cgcgccatgg
     1081 caaatatcgc tttgccgata gagctatgac cgccagaaac atgcttatga gtataaaaga
     1141 gcaaacgtta atgacgcctt acctacagtt tgaccgcaac cagtgggcag ctcagcgtga
     1201 ttccgtacct atgacgttat cggaagatga gatcgcccgt ctcaaaggta ttaatgaaga
     1261 tctctcgtta gaagaagttg ccgagatcta tttacctctg tcacgtttgc tgaacttcta
     1321 tataagctcg aatctgcgcc gtcaggcagt tctggaacag tttcttggta ccaacgggca
     1381 acgcattcct tacattatca gtattgctgg tagtgtcgcg gtggggaaaa gtacaaccgc
     1441 ccgtgtattg caggcgctat taagccgttg gccggaacat cgtcgtgttg aactgatcac
     1501 tacagatggc ttccttcacc ctaatcaggt tctgaaagaa cgtggtctga tgaagaagaa
     1561 aggcttcccg gaatcgtatg atatgcatcg cctggtgaag tttgtttccg atctcaagtc
     1621 cggcgtgcca aacgttacag cccctgttta ctcgcatctt atttatgatg tgatcccgga
     1681 tggagataaa actgttgttc agcctgatat tttaattctt gaagggttaa atgtcttaca
     1741 gagcggaatg gattatccac acgatccaca tcatgtattt gtttctgatt ttgtcgattt
     1801 ttcgatatat gttgatgcac cggaagactt acttcagacg tggtatatca accgtttcct
     1861 gaaattccgc gaaggggctt ttaccgaccc ggattcctat tttcataact acgcgaaatt
     1921 aactaaagaa gaagcgatta agactgccat gacattgtgg aaagagatca actggctgaa
     1981 cttaaagcaa aatattctac ctactcgtga gcgcgccagt ttaatcctga cgaaaagtgc
     2041 taatcatgcg gtagaagagg tcagactacg caaataattt gcaggggagc gaatactccc
     2101 ctttcttatt tttctgcact acgcagggat atttcaccgc ccatccaggg ttttattatt
     2161 ccatcctgct caagtaataa agccccctgt ttgtctattc cgcgtgaaat gccaaatatt
     2221 tctttatcac caatgataag tttcactggg cgattaataa aattatccag cttttcccag
     2281 cgcgacagat aaggtgccaa tccttcttgt tcgaagagtt ccaacgcagc acgtaattca
     2341 cgtattagca tggccgccaa cgtattacga tcgagattga tccccgcttc ctgcagcgtg
     2401 atccacccct gattaacgac actctcttca acacggcgca ttgccatgtt gatcccggct
     2461 ccaatgacta tttgcgccgc atcgccagtt ttgccagtca gctccacaag aatgcctgcc
     2521 agcttgcgat cctgcagata gaggtcatta ggccatttga cacgaacttt atctgctccc
     2581 agcttgcgta atacttccgc catcacgata ccgataacca gacttaaacc aatcgccgcc
     2641 gccgggcctt gttccagacg ccagaacatc gacaaatata agtttgcgcc aaaaggcgaa
     2701 aaccatttcc gcccccggcg accacggcca gcctgctggt attctgcaac acaggcatcg
     2761 cccgatttaa gctctccgat acgatcaaga aggtactgat tcgtggagtc aataactggc
     2821 agcacggcta cactaccgcc atccagctga cccaatatct gttcagcatt aagtaactgg
     2881 atgggctcag gcaggctgta tcctttaccc ggaacggtaa agacatcaac gccccagtca
     2941 cgcagtgtct gaatgtgttt attaatagcc gcccggctca ttcccagcgt ttcacccaac
     3001 tgctcgccag agtgaaattc accgttcgct aacagggcaa tcaatttcag tggcacggtg
     3061 ttatccttca tgaaattgtc tccactgcgc tcacttcacc tgatgcacca ataaagcgga
     3121 cttcaggctc aagccagaca ttaaattttt cacccacttt ttgtcttaca tggtgtgcca
     3181 gttgcaccac atcttcgctt tttgcattgt cttcattaat gagaactaac gcctgttgac
     3241 ggtgcaccgc agccccaccc atttgcatcc cttttagctg gcactgatca ataagccaac
     3301 ctgctgccag ttttactgaa ccacccgcct gggggtaatt tggtgctgtt ggaaattgcg
     3361 ccagtaatgc tttagccgtt tcggcagata caacagggtt tttgaagaaa ctaccggcat
     3421 tgccattcac ttttggatca gggagtttgg tggtgcgcat atgacacacc acattaaata
     3481 cttgctgtgg cgttactgtt gtaggatcca gacgagttaa gtcaccatac gttagtacag
     3541 gttgccactc ttttagcaga cgcagaccta cggctacaat ggcgaagcga tcctggtatt
     3601 catgtttaaa aatactgtcg cgatagccaa aacggcactc tttggcagtt aagcgcactt
     3661 gcttgcctgt cgccagttca acacaatcaa cataagcgca aactcgctgt aattctacgc
     3721 cataagcacc aatattctgg ataggtgatg agccgacaca accaggaatt aatgccagat
     3781 tttccagacc aggcatacct tcctgcaaag tgtattttac caggcgatgc cagttttctc
     3841 cggctcctac atgtaaatac cacgcatcag gttcatcatg aatttcgata cctttgatcc
     3901 ggttgatgat caccgtgccg cgatagtctt ccagaaaaag tacattactt ccttcaccca
     3961 gaataagaac gggttgtcct tctgcggttg catactgcca ggcattgagt aattgttgtt
     4021 cgtcttcggc acatacaatg tgctgagcat tatgatcaat gccaaatgtg ttccagggtt
     4081 ttaaggagtg gttcatagct gctttcctga tgcaaaaacg aggctagttt accgtatctg
     4141 tggggggatg gcttgtagtt atgacgacag ggagagtttg tag
//
LOCUS       DAJTMC010000239         4080 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.239, whole genome shotgun sequence.
ACCESSION   DAJTMC010000239 DAJTMC010000000
VERSION     DAJTMC010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4080
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.239"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            32..931
                     /gene="pgrR"
                     /locus_tag="OO130_003969"
     CDS             32..931
                     /gene="pgrR"
                     /locus_tag="OO130_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HCR5367175.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSALASLSELQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSVPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
     gene            1268..2881
                     /gene="mppA"
                     /locus_tag="OO130_003970"
     CDS             1268..2881
                     /gene="mppA"
                     /locus_tag="OO130_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HCR5367176.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMKQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRIMYIVKH"
     gene            complement(2932..3963)
                     /gene="ynaI"
                     /locus_tag="OO130_003971"
     CDS             complement(2932..3963)
                     /gene="ynaI"
                     /locus_tag="OO130_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HCR5367177.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSIINYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPPEQGR"
BASE COUNT         1146 a          895 c          960 g         1079 t
ORIGIN      
        1 aaatgattaa tagattgcat ggagatactg aatgaagcgt gaagaaatcg ctgatctgat
       61 ggcgtttgtc gtcgttgcag aggagcgtag cttcactcgt gcagcagccc gcctgagcat
      121 ggcgcagtca gctttaagcc agatagtgcg tcgtatagaa gaacgattgg gattgcggct
      181 tctgacgcga accacgcgca gcgttgttcc aactgaagcg ggcgagcatc ttttgtctgt
      241 tcttggccct atgttgcatg acatagattc agccctggca tccctgagcg agctgcagaa
      301 ccgcccatcc gggacaatac gtattactac tgtagaacat gcagcaaaaa cgatattgtt
      361 accagcaatg cgcacattcc tgaaatcgca tcctgaaatt gatattcagc tcaccattga
      421 ttatggtttg accgatgtcg tttctgaacg ttttgatgca ggcgtccgtc tgggtgggga
      481 gatggataaa gatatgatcg ccattcgaat cgggccagat ataccaatgg ctattgttgg
      541 ctcaccggat tatttttctc gccgaagtgt tccaacgtca gtgtcacaat taatagatca
      601 tcaggcaatt aatttgtatc ttcccacatc gggtacagca aatcgctgga gattaatacg
      661 cggtggacgt gaagttcgtg ttcgcatgga aggtcagctt ttactgaata cgatagacct
      721 gatcattgat gctgcaattg atgggcatgg attggcgtat ctaccttatg atcaggttga
      781 gcgggctatt aaagaaaaaa aactgatacg tgttttggat aaattcacac cagatttacc
      841 cggttatcac ctgtactatc cacaccgtcg acatgctggc tcggcattct cattatttat
      901 agataggctg aagtataaag gtgctgttta gcactacttg ctgatacatt aatttaattt
      961 ctctcttaac gtattctcag ttcctttcaa cgttttggtt attttttatt cttcgtacaa
     1021 tggcgacaga tgctgatttt gataccgaaa actggtttga acgtgcgaag cctgaacgta
     1081 gtattcggtg ttctatgtgc tttgccgcat tttggcgtcg attatttaca ctaaatctga
     1141 tttaatatat tgatacataa aacattttat gcttcctgtg tcactttttt gatggaagtt
     1201 gtttgcattt ctttaaggcg aaacaaataa ttacgcatca attttaatgt cggttagagg
     1261 gaaacttatg aagcactctg tttcagtcac gtgttgtgcg ctgttggtca gcagcatttc
     1321 tctttcgtat gctgcagaag ttccgagcgg cacagtactg gcagagaagc aggagctggt
     1381 gcgccacatt aaagatgagc ctgcgtcgct ggatcccgct aaagccgtgg gcctgccaga
     1441 gattcaggtc attcgcgatc tgtttgaagg actggtgaat cagaacgaga aaggggagat
     1501 tgtccccggc gttgcgactc agtggaaaag taatgacaac cgtatctgga cttttaccct
     1561 gcgcgataac gcaaaatggg cggatggcac accggtaacg gcgcaagatt ttgtctacag
     1621 ctggcagcgt ctggtggacc cgaaaacgct atcgccattt gcatggtttg ccgcgctggc
     1681 gggaatcaac aacgcacagg cgattattga tggtaaagct acgcctgacc agcttggcgt
     1741 caccgcagtt gatgcccata ccttgaaaat tcagcttgat aaaccgttgc cgtggtttgt
     1801 gaatttaacc gctaactttg ccttcttccc ggtgcaaaaa gccaacgtag aaagcggtaa
     1861 agagtggacg aaacccggaa atctgatcgg caatggcgct tatgttctta aagaacgcgt
     1921 agtcaatgaa aaactggtcg tggtaccgaa tacccattat tgggataacg ccaaaacggt
     1981 actgcaaaaa gtgaccttcc tgccaattaa tcaggaatcc gcagccacta agcgttacct
     2041 cgcgggggat attgatatca ccgaatcctt cccgaaaaat atgtatcaga agctgttgaa
     2101 ggatattccg gggcaggttt atacgccgcc gcagctcggg acctattatt atgcgtttaa
     2161 cacgcaaaaa gggccgacgg cagatcagcg cgttcgcctg gcattaagta tgacgataga
     2221 tcgccgcctg atgaccgaaa aagtgttggg aacgggcgaa aagccagcgt ggcattttac
     2281 accagatgtt accgccggat ttacgccgga accttcgccg tttgaacaaa tgagtcagga
     2341 agaactgaat gcgcaggcaa aaactttgtt gagcgcagct ggttatggtc cgcaaaaacc
     2401 gctgaagctg acgcttttgt ataacacttc agaaaaccat caaaaaattg cgattgctgt
     2461 agcatcgatg tggaaaaaga accttggcgt agatgttaaa ttgcaaaatc aggaatggaa
     2521 aacctatatc gatagccgta acaccggcaa ttttgatgtt atccgcgcct catgggtggg
     2581 ggattataat gaaccctcca ctttcctgac attattaacg tcaacgcatt caggaaatat
     2641 ttcacgcttt aacaatccgg catacgacaa agttctggcc caggcatcga cggaaaatac
     2701 cgttaaagcg cgtaatgccg attacaacgc ggcagaaaaa atcctcatga agcaagcacc
     2761 gattgcacca atttatcaat ataccaatgg acgattaatc aagccgtggc tgaaaggtta
     2821 tcctattaat aacccggaag atgtggcgta cagccggatt atgtatattg tgaagcattg
     2881 atgtgatggg aactggcgtt acccttgtgg ataacgccag tgatgtctga tttagcgccc
     2941 ctgttccggc ggtgtaatgt tatccatata cagcgtctgg ctggggaagg caaagtccgc
     3001 gccgtgtgac tgcacaatat cgataatctt caaataaacg tcttgttgtg cagcaagcca
     3061 ttcagcccat accgtggttt tggtaaagca ataaaccata atattcaatg acgagtcggc
     3121 aaactggttg aaataaacca gtaaggtttg tcgctggtcg atggccgggt gatttttcag
     3181 catctcacgt acggcttcga caataacgcc cacttttgcc gcatcctcat aacgtaaacc
     3241 aatggtcgtg gtaatgcggc ggttggtcat tcgtcctggg ttttctacgc tgatcgacga
     3301 aaacagcgag ttcggtacgt acaatggacg attatcaaag gtcgtaattt tggtaattcg
     3361 ccagccaatt tccgctactg taccttcgat atttctgtcc ggtgaacgga tccagtcgcc
     3421 aatactgaaa ggacggtcga aatagagcat gatcccggaa aagaaattac tcagaatatc
     3481 tttaccggcc ataccgacag ccagaccacc aataccacca aaggtcagca agccagaaag
     3541 gctcatgccg aaatgttcgc cataaagcag aacaagcacc acaataatgg tgattttgat
     3601 aatgcgcgac ataatccgcg cactggtgat atcgcgacct tttttaatct gctgtttttc
     3661 aaactgatta atcagcaaaa atagcttaat cgtcagaata accgcaatca gggacgtaca
     3721 gataaaatcg ataacgcctg gggtgataaa tttgagttta tagttttcta taacataatt
     3781 aataatgcta ccaacagcac tgataattat ggtgtagatt aaaaattgca ccgcatggaa
     3841 taaaaaacct tttcttttac gatttccgcg gcgaaaccaa aagctcatca gaatcaatgc
     3901 tgcgcagcta ccgaaaataa tgaccagatt aagcgcatta tttgtaaaca gttcagcgat
     3961 cattgtttta tcaggctcct ccagataatt gtcgtcatgc cggaaccccc tggcggggct
     4021 attttaccgc gacaattcat tcagatcatc aatagtcagg gaaggaagta gcaacattag
//
LOCUS       DAJTMC010000240         4078 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.240, whole genome shotgun sequence.
ACCESSION   DAJTMC010000240 DAJTMC010000000
VERSION     DAJTMC010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4078
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.240"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(<1..1046)
                     /locus_tag="OO130_003972"
     CDS             complement(<1..1046)
                     /locus_tag="OO130_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HCR5367178.1"
                     /translation="MREINMLRNISSCLFPHISTITSPNHYLSEWDDWEKQGLPEEQR
                     TEAVRRLRACLTSKGHKLDLRALALSSLPVLPACIKKLDVSCNKLTILTDLPENIKEL
                     IARDNFLTHISALPHYLITLDVSENQLENLPLLPDTIKSLSAEYNRLSTLPSLPLNLK
                     KLEVRNNELQTLPSLPSNLKILKVAHNHLTELPPLPRRLQLLFAYSNRLSNLPNIQEN
                     IIMRRFFYFENNQITTIPTNLFRLDPHITIEIANNPLSDQTLLFLIQQTSVPNFNGPQ
                     FRISLSDQNRLFLRQMLPQNLHSRHIRVITEGGQNFQIPPLPETVAAWFPEADRREVS
                     TQWTSFSTEENSRA"
     gene            1537..1647
                     /locus_tag="OO130_003973"
     CDS             1537..1647
                     /locus_tag="OO130_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001344347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA invertase"
                     /protein_id="HCR5367179.1"
                     /translation="MLIGYVRVSTNDQNTDLRRNALNCAGYELIVERMGA"
     gene            1927..2922
                     /gene="pgl"
                     /locus_tag="OO130_003974"
     CDS             1927..2922
                     /gene="pgl"
                     /locus_tag="OO130_003974"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HCR5367180.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTIEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIAAGQKSHLISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(2963..3915)
                     /locus_tag="OO130_003975"
                     /pseudo
     CDS             complement(2963..3915)
                     /locus_tag="OO130_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
BASE COUNT         1093 a          829 c          957 g         1199 t
ORIGIN      
        1 aatgcccggg aattctcctc ggtggaaaaa gaagtccatt gtgtagaaac ctcccgacga
       61 tctgcttcag gaaaccaggc tgccacagtt tcgggaagag gggggatctg aaagttctgc
      121 cccccttcag tgatgactct gatatggcgc gaatgtaaat tttgcggcaa catctggcgt
      181 aaaaacagtc tgttttggtc tgacagggaa atacgaaact gaggcccgtt aaaatttgga
      241 accgaagttt gctgtattaa gaatagcaga gtttgatctg ataaggggtt atttgcaatc
      301 tcaatagtta tatgaggatc taaacgaaaa agatttgtcg ggattgtagt tatttggttg
      361 ttttcaaaat aaaaaaatct tctcatgata atattttctt ggatgtttgg taagttgctt
      421 aatctattgc tatatgcaaa aagaagttgc agtctcctag gtaaaggggg cagttcagta
      481 agatggttgt gcgcaacctt aagtatctta agattagaag gcagagatgg aagagtttgc
      541 agttcgttgt tcctaacctc aagttttttt aaattcaagg gtaatgaagg cagtgtggat
      601 aacctattat actctgcgct tagtgatttg atggtgtctg gtaataacgg cagattctct
      661 aattgatttt cggacacatc caaagttatt agataatgtg gtaatgcaga tatatgtgtt
      721 aagaaattat ctcttgcaat aagttcttta atattttcag gtaaatcagt aaggatggtt
      781 aatttattac agctcacatc aagcttttta atgcaagcag ggagtacagg taacgaggaa
      841 agcgccaagg ctcgcaggtc cagtttatgc cccttagagg taagacatgc acgaagtctt
      901 cttaccgcct cagtacgctg ttcttccggt aacccctgtt tctcccaatc atcccattcg
      961 gacaaataat ggttggggga tgtaattgtg ctgatatgtg gaaataaaca ggatgaaata
     1021 tttctgagca tattaatttc tcgcatatgg atatggaatt agattgtgaa taaattgtat
     1081 ttaatgtatc tctcctaatg aggagggtgt ttcagaatat tcgccataag ggaatgagtg
     1141 ttatatcatg ctttctatcc tggtaagtat atttgctttt ttcgtatatt tggttcatct
     1201 ttttttgatg ttttacatat tcacaatgca gaggatttat accagaagac attagtcttg
     1261 agtaagatac tacaacatct ccaactattt tagccagcct gactacttga cttggtaata
     1321 tttgttgcta ataatcatgt ataaaataag cttatataca tagtaacttc agcaaaggat
     1381 ttagtaatga tccaaatcat atatcaatgt actgtttttt tctttaaaaa aacagtacat
     1441 tctattttat aatgactgtg tattcccatg gaaggagatc acccaattct cccccccgac
     1501 ttttgacgca gtgtcttgtt aacaggggga tttaccatgc ttattggcta tgtacgcgta
     1561 tcaacaaatg accagaacac cgatctacga cgtaatgcgt tgaactgtgc aggatatgag
     1621 ctgattgttg agcgaatggg agcttgattg gaagttgtta gggtatggtg tttacggtcg
     1681 ttgatatggc gaacaaacgc ggggagcgtc acgctcccta aatggtcgcg aaaatatctg
     1741 tggcattgtc aaatttgtga cggtgactaa tattttaatg atatgccatt cgaaatggtg
     1801 tgctaacgta ttgatttaat tgtgacgcga tgttgtattt tatcgcgttt tgttgtctgt
     1861 ttttcattcg cttgatgttg ctatttacat gaaaaataaa gaatttattg tttttgaggt
     1921 gtgtttatga agcaaacagt ttatatcgcc agccctgaga gccagcaaat tcacgtctgg
     1981 aacctgaatc atgaaggcgc actgacgctg acacaggttg tcgatgtgcc ggggcaggtg
     2041 cagccgatgg tggtcagccc ggacaaacgt tatctctatg ttggtgttcg ccctgagttt
     2101 cgcgtcctgg cgtatcgtat tgccccggac gatggcgcac tgacctttgc cgcagagtct
     2161 gcgctgccgg gtagtccgac gcatatttcc accgatcacc aggggcagtt tgtctttgta
     2221 ggttcttaca atgcgggtaa cgtgagcgta acgcgtctgg aagatggcct gccagtgggc
     2281 gtcgtcgatg tggtcgaggg gctggacggt tgccattccg ccaatatctc accggacaac
     2341 cgtacgctgt gggttccggc attaaagcag gatcgcattt gcctgtttac ggtcagcgat
     2401 gatggtcatc tggtggcgca ggaccctgcg gaagtgacta ccattgaagg ggccggcccg
     2461 cgtcatatgg tattccatcc aaacgaacaa tatgcgtatt gcgtcaatga gttaaacagc
     2521 tcagtggatg tctgggaact gaaagatccg cacggtaata tcgaatgtgt ccagacgctg
     2581 gatatgatgc cggaaaactt ctccgacacc cgttgggcgg ctgatattca tatcaccccg
     2641 gatggtcgcc atttatacgc ctgcgaccgt accgccagcc tgattaccgt tttcagcgtt
     2701 tcggaagatg gcagtgtgtt gagtaaagaa ggcttccagc caacggaaac ccagccgcgc
     2761 ggcttcaatg ttgatcacag cggcaagtat ctgattgccg ccgggcaaaa atctcatctc
     2821 atctcggtat acgaaattgt tggcgagcag ggactactgc atgaaaaagg ccgttatgcg
     2881 gtcgggcagg gaccaatgtg ggtggtggtt aacgcacact aaccgctgat ttaaccggcg
     2941 cagtctctcc tgcgccggtg tattaaccta tctcctgtaa cgcatgtctc tggcgttcga
     3001 cgatattggt ccacaaattg tctttatcgt cagtccacaa attaatcagc aaggcaaaaa
     3061 agcgttcagc tgccggagaa agcacggcat ctttgcggcg aataatcccc aatgtccgac
     3121 gtataacggg ttccaccagt gggataccaa taatcgaaga atagggcgca tggggcattg
     3181 ccagaccagg aagcgccgaa atccccagtc ctgcctccac tagtcctaat gacgtcgaaa
     3241 gatgacgcac ttcgtaaaac cagtccagct tccagggctt gtcggccagc tgttgttcta
     3301 tcaacagtcg gttgccgctg gaggagcgta cgccaatcat tttgtaaccc actaactcct
     3361 gccattctac aagctgcttt ttggccaatg gatggtcacg ccgacaggca gcacgaacgg
     3421 ctcgttgacc agtggggtaa aatcaatgga tgaatttgtg acgttgttca tgtttatgcc
     3481 aaagtctgat tcgttacaca gcaccgattc catgcaatta ttcgtgcctt gttccagaat
     3541 ccgcacttta atattgggat acagctcatt aaatttaccg attgccagcg gtaaaaaata
     3601 aaatactgcg gtcggaatac acgctaacgt caccatacca cgatgataag cattcatatc
     3661 acgaatatta aaaagcgttt catcgaattt ctttattaat tcccgcgcct ccgggagcaa
     3721 cctttttcct gcttttgtta acgtaacctt gcgagttgta cgctcaaata gttgaatatg
     3781 taaatcttcc tccatctttt ttattcttcg cgttaatgca ggttgcgtaa tattgagtaa
     3841 tttagcggca ttgttaaacg aactggactc tgccagtatg acaaatgcct tcatacttga
     3901 taattcatgc ttcattatta ctccggaaaa tggaagcgac gattttgggt ggctggccgt
     3961 taaaaatttt aactgcattt agccaactta aattaatgaa aaaatgttat taatcgttga
     4021 gctaaagtca ttagagatgc tttgccctta atgtaaccat atcgcaataa gggtaatg
//
LOCUS       DAJTMC010000241         4071 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.241, whole genome shotgun sequence.
ACCESSION   DAJTMC010000241 DAJTMC010000000
VERSION     DAJTMC010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4071
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.241"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            112..615
                     /gene="yeaK"
                     /locus_tag="OO130_003976"
     CDS             112..615
                     /gene="yeaK"
                     /locus_tag="OO130_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HCR5367181.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTTDYLRIAQPEL
                     VNFRRTA"
     gene            complement(616..720)
                     /locus_tag="OO130_003977"
     CDS             complement(616..720)
                     /locus_tag="OO130_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5367182.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            889..1335
                     /locus_tag="OO130_003978"
     CDS             889..1335
                     /locus_tag="OO130_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HCR5367183.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(1292..2113)
                     /gene="nimR"
                     /locus_tag="OO130_003979"
     CDS             complement(1292..2113)
                     /gene="nimR"
                     /locus_tag="OO130_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HCR5367184.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVEHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RTMVEMMAKGPVEWGSLGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            2210..3406
                     /gene="nimT"
                     /locus_tag="OO130_003980"
     CDS             2210..3406
                     /gene="nimT"
                     /locus_tag="OO130_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005117270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HCR5367185.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFTGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLAAPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMVVPLA
                     LNGFGWQGALLMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGSNSLVYYVIIGWLPAILISHGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGNWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     RSYPVL"
     gene            3446..3793
                     /locus_tag="OO130_003981"
     CDS             3446..3793
                     /locus_tag="OO130_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HCR5367186.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFTTFREQYLAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(3815..4069)
                     /gene="yoaF"
                     /locus_tag="OO130_003982"
     CDS             complement(3815..4069)
                     /gene="yoaF"
                     /locus_tag="OO130_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HCR5367187.1"
                     /translation="MKIISFVLPCLLVLAGCSTPSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVSTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
BASE COUNT          936 a         1041 c         1024 g         1070 t
ORIGIN      
        1 catgatgcgt ataaagcttc cgatgagcgt ttatatgtca ataagcagaa caaaaacagc
       61 cgttcatgat aaccttctgt ggttgtttgc ttgtaatctc aggagcgtga aatgactgaa
      121 atggctaaag gaagcgtgac gcatcagcgg ttaatcgctt tattatcaca agaaggtgct
      181 aacttccgcg tcgttaccca tgaagcggta ggtaaatgtg aagcggtgtc agaaattcgt
      241 ggcaccgctt taggccaggg agctaaggca ctggtatgta aagtcaaagg caatggcgta
      301 aatcaacatg ttctggcgat cctcgctgcc gatcaacagg ccgatctgag ccaacttgcc
      361 agccatatag gcggattacg cgcttcgtta gccagccctg ctgaagtcga tgaactcaca
      421 ggctgtgtct tcggcgcgat cccccctttc agcttccatc caaaactcaa actggttgcc
      481 gaccctctac tcttcgaacg atttgatgaa atcgccttca atgcagggat gctggataaa
      541 tccgttattt tgaaaaccac ggattatttg cgcattgcgc aaccagaact ggtcaatttc
      601 cgccgcactg cgtaactagc ctgtccgttc aataagcaga acggacaaaa ccagcacaac
      661 agcaatggca aaaaacgatg acgtgataat cagtgtctcg acgaacattt gatcgttcat
      721 agcatgcccc cgggagatag ctgttttcac gttattgtta gcgtgcacaa atggcagttt
      781 gatgacagtt cgcctgattt ttatgcaaaa aacgtaaaga ttttctactt ccttcctgca
      841 gcaagcgtaa agtaagcagg cttattattt tttggcaagg aaaccacgat gtttgatgtc
      901 actttgctga tcctgctcgg gttagctgcg ctgggcttta tcagtcataa caccactgtc
      961 gccgtttcaa ttctggtgtt gatcattgtc cgcgtcacac cgttaagcac ctttttcccg
     1021 tggattgaaa aacaggggct aagtatcggg ataatcattc tgacgattgg cgttatggcc
     1081 cccatcgcca gcgggacgct accgccctca accttgattc actcgtttct gaactggaaa
     1141 tcgctggtgg caattgcagt aggcgtgatt gtctcctggc tgggtgggcg cggcgtgacc
     1201 ttaatgggca gccagccgca actggtcgcc gggctgctgg taggcactgt tttaggcgtg
     1261 gcgctgtttc gcggcgtacc ggtcggcccg cttattgccg cgggtctggt ttcgctgatt
     1321 gtggggaaac agtagttaat cccgcaatat atcggcctgg cgtttgcccc aggcctttct
     1381 taaacatagt aataaaggcc gttgttgaat cataccccag cgtatgagca actttctgta
     1441 cagtatcgcc tttcactaat ccctgaagtg ccataatcag ctgtaactgc tgacgccact
     1501 ggcgaaaact caaccccgtc tcttttacga tcagtcgcgc aagattgcgt tcactcatcg
     1561 caaaaaagcc agcccattgc cctaatgacc cccactcgac gggccctttc gccatcatct
     1621 ccaccatcgt gcggatttta ggatgagaag aaacgggcag gtgcagctgt tgttgtggct
     1681 gctgagggag ttcatcaaac aggacctgta ttaatctgcg ggtcatcggc tcagcacgct
     1741 gagtcgtcgt cctgtttgcc agggttaaaa tcaattcccg gcataagggc gatattttca
     1801 atgtgcaaca tgttgtcggc attgtcactg cgctgggttc gataaacaga aaacagagtt
     1861 cagcgttagc tgtcacttga ttgctatgtt ccaccccgcc cggtatccaa accgcatatt
     1921 gcggcggcac catccacaaa gcattttcca ccgtacaggt aattgcacca tgtaatgcta
     1981 gtatcagctg tcctttgcgg tgctgatgta caggactggt taactcatcc gttccggcgt
     2041 gaatacaaaa tgcgaccgcg gcttcatggt gtcggtcggg ttcatagcca ttgagattca
     2101 acctgtgcat cattttgtcc gaatttagcg ataatttgtc attttagctt gattcaacat
     2161 aacaataaaa acggtaaggt acagcctcgt ttgtaacaat gagaagcata tgacctgttc
     2221 aacttcatta agcggcaaaa acaggattgt ccttatcgct ggcattctga tgattgccac
     2281 aacattacgc gtcaccttta ccggcgcagc accgttactg gatacgattc gttccgctta
     2341 ctcgctgacg acagcgcaaa ccggcttatt gaccaccctg ccattattgg cctttgcgct
     2401 aatctcacct ttggccgccc cggtagcgcg acgttttggt atggaacgta gcctgtttgc
     2461 cgcgttactt ttgatctgtg ctggtatcgc aattcgctct ctcccttcgc cttacttatt
     2521 atttggcggt acagcggtca ttggcggtgg gattgcatta ggcaatgtct tactgccagg
     2581 attaattaaa cgcgatttcc ctcattccgt cgccagactt accggcgcat attccctgac
     2641 aatgggagct gcggcggcac ttggatcggc tatggtagtg ccgctggctt tgaacggttt
     2701 tggctggcaa ggcgcgttgc tcatgctgat gtgttttcct ctgctggctc tttttttatg
     2761 gctgccacag tggcgaagcc aacaacatgc aaatttgagt acctcgcgcg ccttacatac
     2821 tcggggtatc tggcgttcgc cgcttgcctg gcaggtcaca ttgtttcttg ggagcaactc
     2881 actggtctat tacgtgatta ttggctggct tccggcgatc ctcatcagtc acggctatag
     2941 cgaagcacag gcgggttcac tgcatggttt gctgcaacta gccacagcag ctcccggttt
     3001 gctgatccct cttttcttac atcatgtgaa agaccagcgt ggtattgcag cgttcgttgc
     3061 cttgatgtgc gcagtgggcg ctgttgggct ctgctttatg ccagcgcacg cgatcacctg
     3121 gactctgctt ttcggttttg gttccggcgc aacaatgata ctggggttga cgttcattgg
     3181 tcttcgggca agttctgcgc atcaggcggc ggcactctcg gggatggcac aatccgtcgg
     3241 gtatttgctg gcagcctgtg ggccgccgct aatgggtaaa atacacgatg ctaacggtaa
     3301 ctggtccgta ccactgatgg gtgttgcaat actttcacta ctgatggcga ttttcggact
     3361 ttgcgccggg agagacaaag aaattcgctc atatccggtg ctatagtgac gtgacaaatc
     3421 atgcgtgaaa gggagaacaa acacgatgaa tattcagtgc aaacgcgttt atgatccggc
     3481 tgaacagagc gatggttatc gcgtactggt cgaccgcctc tggccgcgcg gtatcaaaaa
     3541 aaccgattta gcccttgatg agtgggataa agaaatcacg ccgtcaacgg aactgcgcaa
     3601 agcctttcac ggcgaagtcg tcgattttac aacctttcgc gagcaatatc ttgcagaact
     3661 ggcgcaacac gagcaagaag gaaagcggct ggcggacatc gccaaaaaac agccgctgac
     3721 cctgctctac gcagcaaaaa acaccacgca gaaccatgcg ctggtgctgg ccgactggct
     3781 acgtagcttg tgattttagt acagcatccg gcggttattt ttcaccagcc ggatggtccc
     3841 gtcgccacaa tgcccattca tcaatcgttt caccgcccgg taatttgcaa ttgttgctga
     3901 ccccttgcgc tgtcgacact ggaatgagcg tcccgccctt ctgctggcaa tagaccgacg
     3961 ccggatttgc cataccaatc tgcggcggtt taggtgcttc tggctgagaa ggggttgaac
     4021 aaccagccag gaccagcaag caaggcagaa caaaactgat aattttcatt t
//
LOCUS       DAJTMC010000242         4066 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.242, whole genome shotgun sequence.
ACCESSION   DAJTMC010000242 DAJTMC010000000
VERSION     DAJTMC010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4066
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.242"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(189..656)
                     /locus_tag="OO130_003983"
     CDS             complement(189..656)
                     /locus_tag="OO130_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014526575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5367188.1"
                     /translation="MRIDISHQTRHTPPNMLPREQNCVAMALSACFRQQLNPVVNSLL
                     KERIIHSPKELEHDNAVIRALQKLQIQEVCNSTLWETAKQQLLQKSDGRYFAINSKHL
                     AFPGPGESHAFCCIKYKNAIGINGNNAETQSTHYQPYPYDKVSIWGPFPHNLT"
     gene            complement(1724..1915)
                     /locus_tag="OO130_003984"
     CDS             complement(1724..1915)
                     /locus_tag="OO130_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HCR5367189.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADDQADICEALHDH
                     ADELYRSCLARFGDDGENL"
     gene            1983..2306
                     /locus_tag="OO130_003985"
     CDS             1983..2306
                     /locus_tag="OO130_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HCR5367190.1"
                     /translation="MLTIRVTDEEHARLLARCEGKQLASWMRKVCLGAPPSKTSGLPT
                     LAPPLLRQFASVGNNLNQIARKINSGQWSGHDRVHVVAALMAIGRELSELRDEVRKQG
                     ERDDS"
     gene            2312..3013
                     /locus_tag="OO130_003986"
                     /pseudo
     CDS             2312..3013
                     /locus_tag="OO130_003986"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2985..3545
                     /locus_tag="OO130_003987"
     CDS             2985..3545
                     /locus_tag="OO130_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HCR5367191.1"
                     /translation="MSNLLQMGTDFEKKLKERAVSTENILNSEFRKLEASVDRELNLN
                     GQKIRDAISAHTAAVKEQLETLNSAVNAQFSATENELARRQEELLWKLVKGRILYPSL
                     TALCVTGGIFLASWGLIQWQESRIAANILAIRDQEETLAKLREKTWGVTYHEGRNGKF
                     LVLPSGVKGENNWTVEKKNAVRLVRE"
     gene            3552..3785
                     /locus_tag="OO130_003988"
     CDS             3552..3785
                     /locus_tag="OO130_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HCR5367192.1"
                     /translation="MTELEMHLLNALEQLQQDYTQRLKDWESAFTDLQHMFELTRQEN
                     GQLREQCDDLSQQVQRLVEQVDHLSRLFITNSR"
BASE COUNT          971 a          934 c         1228 g          933 t
ORIGIN      
        1 aaaaaaaaca gtgtcatgag gtttacgcag gcaaaaccag cgttattcac atggctgaat
       61 agctatgcat acggttaaaa tttatcaggt gcgatcgcgg cagcttttcg ggtggtttgt
      121 tgctattttt acctgtcagc agccgtgatc gcgctgaacg catttcagcg gtgcgtacaa
      181 ttaagatatt acgttaaatt gtgagggaat ggtccccata tactgacttt atcataggga
      241 tagggttggt agtgggtcga ttgggtttcg gcattgtttc cgttaatgcc aattgcattt
      301 ttgtatttga tgcaacagaa ggcgtgagat tctcctgggc ctggaaaggc taagtgttta
      361 gaattgatgg caaaatatct tccgtccgat ttttgaagta gttgttgttt ggctgtctcc
      421 caaagggtgc tgttacaaac ttcttgaatt tgtagttttt gcagggctct tattactgcg
      481 ttgtcatgtt ccaattcttt aggggagtgt attattctct cttttaataa gctgtttacc
      541 acagggttta gttgttgtct aaagcacgca gataatgcca ttgctacaca attttgttcc
      601 cgaggaagca tgtttggcgg ggtatgccgt gtttgatgtg atatgtctat tctcatagaa
      661 ggtctctggc ttatagcatc aacaaatacg gctataaaac aaaattttca tagagtgatt
      721 caagatcttc ttgagatcct tttttgcggc gcgtaatctg ctgcctgcaa acaaaaaaac
      781 caccgctacc aacggtggtt tgtttgccgg aatctagagc taccaactct ttcttccgaa
      841 ggtaactggc ttggtggagc acagatacca aatactgtcc ttctagtgta gccgcagtta
      901 ggccaccact tcaagaactc ttaatatctc aatccacctt gtctagttac caatggctgc
      961 tgccagtggc gttaagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     1021 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     1081 ctacaccgaa ctgagatacc aacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     1141 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     1201 gccaccaggg ggaaacgcct ggtatcttta agtcctgtcg ggtttcgcca cctctgactt
     1261 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccaccggc
     1321 gcggcctttt tcgggctctt gttttttgct cacatgtttc ttttggcttt atcccctgat
     1381 tctgtggata actgtgttac cgtctttgag tgcgctgata ccgctcgccg cagccgaacg
     1441 accgagcgta gcgagtcagt gagcgaggaa gcggaagagc gccggaagct acattttctc
     1501 cttacgcatc tgtgcggcat ttcacacccg gcatggtgca cttttcatac aatccgcact
     1561 gatgccgcat aagtaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg
     1621 cgccccgaca cccgctaaaa cctgctgacg cgccctgacg ggcttgtctg ctcccggcat
     1681 ccgctcacag gccagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccat
     1741 catccccgaa gcgtgcgagg cagctgcggt aaagctcgtc agcgtggtcg tgaagcgctt
     1801 cgcagatgtc agcctggtcg tcggcgtcca gttcattcag tttttccagc agggtcagcg
     1861 tctggcttct gatgaagcgg gccatgttaa gggcggtttt ttcctgttta gtcacagttc
     1921 tgtttctcca tgtgtgggga gtctgtcgga ggtttttaat gataccggtg aaacgcgaaa
     1981 ggatgctcac gatacgggtc acggatgagg aacatgcgcg gctgctggca cgttgtgagg
     2041 gaaaacaact tgcgtcatgg atgcgtaagg tctgcctggg ggcaccgcca tcgaaaacgt
     2101 caggtcttcc gacactggca ccgccgctgt tgcgccagtt cgcttcggtc ggcaataacc
     2161 tgaatcagat agcccggaag ataaacagcg gacagtggtc ggggcatgac cgggttcatg
     2221 tggtggctgc actgatggcc atcggacgtg aactgagtga gctgagggat gaggtccgta
     2281 aacagggaga gcgggatgat agttaagttt catgccaggg ggaaaaggcg gtggcagtgg
     2341 tccggttgat tacctgctgg gccgggagcg taaccgggaa ggggcgagag ttctgcgggg
     2401 tgctcccgaa gaggtgcggg aactcatcga tgccacgccg tttgcgaaaa aatacacctc
     2461 gggggttctg tcttttgcag agcagaccct gccaccggga gagcgtgaaa gggtgatgga
     2521 gagctttgag cgggtactga tgcccggtct tgaaaaaaat cagtacagca tcctgtgggt
     2581 ggagcaccag gacaaggggc gtcttgagct gaattttgtc atcccgaata tggagctggc
     2641 gagcggaaaa cggctccagc cctattacga ccgagcggac cgtccgcgaa tcaatgcctg
     2701 gcagacactg gtgaatcacc attacgggct gcacgacccg aacgcaccgg agaacaggcg
     2761 gacacttgtc accccgaata atctgccgaa agcgaaacag gaggccgcag aagcgattac
     2821 gaggggactg gaggctcttt accatgccgg agcgctaaaa acgcgtcagg atgtcactga
     2881 ggcgcttaca gcggcaggtt ttgaggttgt gaggacaacc aggagcagta tcagtatcgc
     2941 cgacccggag ggcgggcgta atctcaggct gaaaggggcg ctgtatgagc aatcttttac
     3001 aaatggggac agacttcgag aagaaactga aagagcgtgc cgtctctaca gagaacatac
     3061 tgaacagcga gttcaggaag ctcgaagcgt ctgtagacag ggaactgaac ttaaacgggc
     3121 agaaaatcag agacgctatc agcgcacaca cagcagcggt gaaagaacaa ctggagacgc
     3181 tgaacagtgc cgtgaacgcg cagttcagcg ccacagagaa cgaactggcc cggcggcagg
     3241 aagagctgct ctggaagctg gtcaaagggc ggatactgta cccgtcgctg acggcgcttt
     3301 gcgtcacggg cgggattttt ctggcgtcat gggggctgat tcagtggcag gaaagccgga
     3361 ttgcggcgaa catcctggct atcagggacc aggaagagac gctggcgaaa ctccgggaaa
     3421 aaacatgggg tgtgacgtat cacgagggca ggaacgggaa atttctggta ttgccgtccg
     3481 gggtgaaggg ggaaaacaac tggacggtgg agaagaagaa cgcagtaaga ctggtcaggg
     3541 agtaagtgag tatgacagag ctggaaatgc acttactgaa cgccttagag caattgcagc
     3601 aggactatac gcagcggctg aaagactggg agagcgcctt cacggatttg cagcatatgt
     3661 tcgagctgac acgacaggag aacgggcaac tgagggaaca gtgcgacgac ttgagtcagc
     3721 aggtgcagag attagtcgag caggtggacc acttgagtcg gttatttatc acgaacagca
     3781 gataagggat gaacaggaaa ggcaggcacg cctggaacgg gagcgtgcgc ggtcctggca
     3841 tgaagagcag gaacgggaag cacgggtatg gcgtggcccg tcaataggga tatgacggtg
     3901 agttgttccg ggaggttgcc agatggtacc ggaacgcgtt aaaaacgcat ccgggcgcga
     3961 ttctggcgtg tcgtttttgt tgctgtgttt tgcctctttg ccagatcgcg ctcattcctc
     4021 agcctcatcc cgcgatgtct gcgtatgtca tcaactgaaa agatgg
//
LOCUS       DAJTMC010000243         3975 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.244, whole genome shotgun sequence.
ACCESSION   DAJTMC010000243 DAJTMC010000000
VERSION     DAJTMC010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3975
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.244"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            7..3480
                     /locus_tag="OO130_003989"
     CDS             7..3480
                     /locus_tag="OO130_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HCR5367193.1"
                     /translation="MGKGGGRAHTPREAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPESFESSGSETVLGVEVTRAKPVTR
                     TITSANIDRLRVTFGVQSLVETTSKGDRNPSSVRLLIQLQRNGNWVTEKDVTINGKTT
                     SQFLASVILDNLPPRPFNIRMVRETADSTTDQLQNRTLWSSYTEIIDVKQCYPNTAIV
                     GMQVDAEQFGGQQMTVNYHIRGRIIQVPSNYDPEKRTYSGIWDGSLKPAYSNNPAWCL
                     WDMLTHPRYGMGKCLGAADVDKWALYAIGQYCDQTVPDGFGGTEPRMTFNAYLAQQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDVVWPYTNSDVVVDDNGVGFRYSFSAL
                     KDRHTAVEVNYTDPQNGWQTSTELVEDPEAILRYGRNLLKMDAFGCTCRGQAHRAGLW
                     VIKTGLLETQTVDFTLGSQGLRHTPGDIIEICDNDYAGTMTGGRVLSIDAATRTLTLD
                     REVTLPETGTSAVNLINGSGKPVSVDITAHPAPDRIQVSTLPDGVETYGVWGLSLPSL
                     RRRLFRCVSVRENTDGTFAITAVQHVPEKEAIVDNGARFEPQSGSLNSVIPPAVQHLT
                     VEVSAADGQYLAQAKWDTPRVVKGVRFSLRLTSGSGEDSRLVTTAITADTEHRFSGLP
                     LGEYTLTVRAINSYGQQGEPATTTFRINAPAKPATIELTLGYFQITAVPRLAVYDPTV
                     QFEFWFSEAKIADTSQVETSARYLGTGSQWSVSGPHIKPGKDFWFYVRSVNLVGKSAF
                     VEASGRASNDAEGYLGLFREKIGKLHLAQGLWELIDNSQLADEMAEMKTTITETRNEI
                     TQTVSKTLEDQSATIQQIQRVQKDTNDDLAALYMLKVQKTKNGIPYVAGIGAGIEDTD
                     GQPLSNILLLADRIAMINPEDGNTTPLFVAQGNQLFMNDVFLKRLFAVSITSSGNPPT
                     FSLTPEGRLTARNADISGNVNANSGTLNNVTINENCRVLGKLSANQIEGDLVKTVGKA
                     FPRDSRAPERWPSGTVTVRIYDDQPFDRQIVIPAVAFSGAKHEREHTDIYSSCRLIVR
                     KNGAEIYNRTALDNTLIYSCVIDMPAGHGHMTLEFSVSAWLVNNWYPTASISDLLVVV
                     MKKATAGISIS"
     gene            3548..>3975
                     /locus_tag="OO130_003990"
     CDS             3548..>3975
                     /locus_tag="OO130_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HCR5367194.1"
                     /translation="MRKVCAAILSAAICLSVSGAPAWASEHQSTLSAGYLHARTNAPG
                     SDNLNGINVKYRYEFTDALGLITSFSYANAEDEKKTHYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTH"
BASE COUNT          932 a         1025 c         1217 g          801 t
ORIGIN      
        1 gatgtaatgg gcaaaggtgg cggcagggcg cacacaccgc gtgaggcgaa agacaatctc
       61 aaatccacgc agatgatgag cgtgattgat gcgattggtg agggaccgat agaaggcccg
      121 gtgaaaggcc tgcagagtat tctggtgaac aaaaccccgc tgacggacac ggacggtaat
      181 cccgtgatac acggtgtgac cgcggtctgg cgtgccgggg agcaggagca gacaccaccg
      241 gaaagctttg agtcctccgg ctctgaaact gtactgggtg tcgaagtgac cagggcaaaa
      301 ccggtaacac gcaccattac gtcagcgaac attgaccgcc tgcgggtgac cttcggggtg
      361 cagtcactgg tggagaccac gtcaaagggt gaccgtaatc cgtcctctgt ccgtctgctg
      421 attcagttac agcgtaacgg taactgggtg acagaaaagg atgtcaccat taacggcaag
      481 accacctcac agttccttgc gtcggtgatt ctggataatc tgcctccccg cccctttaac
      541 atccggatgg tcagggagac ggcggacagc accacggacc agctgcagaa cagaacgctg
      601 tggtcgtcat acaccgaaat catcgatgtg aaacagtgct acccgaacac ggccattgtg
      661 gggatgcagg tggatgcgga gcagtttggt ggtcagcaga tgacggtgaa ctaccatatc
      721 cgcggtcgca tcatccaggt gccgtcaaac tatgacccgg aaaaacgcac gtacagtggt
      781 atctgggacg gcagtctgaa accggcatac agcaacaacc cggcctggtg cctgtgggac
      841 atgctgactc acccgcgcta cggcatggga aaatgtctgg gggcggcgga tgtggacaag
      901 tgggcgctgt atgccatcgg gcagtactgc gaccagacgg tcccggatgg tttcgggggg
      961 accgagccgc ggatgacctt taatgcgtac ctggcacaac agcgtaaggc gtgggacgtt
     1021 ctcagtgatt tctgctctgc gatgcgctgt atgccggtat ggaacggtca gacgctgacg
     1081 ttcgttcagg accgcccgtc ggatgtggtg tggccgtaca ccaacagcga tgtggtggtg
     1141 gatgataacg gcgtgggatt ccgctacagc ttcagtgccc tgaaggaccg gcacacggcg
     1201 gtggaggtga attacaccga cccgcagaac ggctggcaga cctccacgga actggtggaa
     1261 gacccggaag ccatactgcg ctacggacgc aacctgctga agatggatgc gtttggctgt
     1321 acctgccgcg gtcaggctca ccgtgcaggt ctgtgggtga taaagaccgg actgctggaa
     1381 acgcagacgg tggacttcac gctcgggtca caggggctgc gtcacacacc gggtgacatt
     1441 attgaaatct gtgataacga ctatgctggg actatgaccg gcggacgtgt cctgtccatt
     1501 gatgctgcca cccgcaccct gacgctggac cgtgaggtta ccctgccgga gacaggtaca
     1561 tcggcggtga acctgattaa cggcagtggt aagccggtga gtgtggacat caccgcacac
     1621 cccgcgccgg accggataca ggtcagtacc ctgcctgatg gtgtggagac atacggggtg
     1681 tggggactct ccctgccgtc actgcgccgt cgcctgttcc gctgtgtctc cgtccgggaa
     1741 aacacggatg gcacctttgc catcacggcg gtgcagcacg taccggaaaa agaagccatc
     1801 gtggataacg gtgcccgctt tgagccgcag tcaggctccc tgaacagcgt catcccaccg
     1861 gcagtgcagc acctgacggt ggaggtgagc gcagctgacg gccagtatct ggcacaggcg
     1921 aaatgggaca cgccgcgggt ggtgaagggg gtgcgcttca gtctgcgact gaccagcgga
     1981 agcggagaag acagccgtct ggtgaccacc gctatcactg cggatacaga gcatcgtttc
     2041 agtggtctgc cgctcgggga atacaccctg acagtcaggg caattaacag ttatggccag
     2101 cagggcgaac cggccaccac caccttccgg attaacgcgc cagcaaaacc cgccaccatt
     2161 gaactgacgc tggggtattt tcagataaca gcggtaccgc gtcttgcggt gtatgacccg
     2221 acggtacagt ttgagttctg gttttcggag gcaaaaatcg cagacacatc tcaggtggaa
     2281 acctctgccc gttatctggg gaccggcagt cagtggagtg tatccggccc gcacattaag
     2341 cccgggaagg atttctggtt ttacgtgcgc agcgtcaacc tggtggggaa atctgctttt
     2401 gtggaggcca gtggacgggc cagcaatgat gcagaagggt atctggggct gtttcgggaa
     2461 aaaataggaa aactgcatct ggctcagggg ctgtgggagc tgatagacaa cagccagctt
     2521 gcggatgaga tggcggagat gaagaccacc atcaccgaaa cccgcaatga aatcacacag
     2581 acggtcagta aaacgctgga ggaccagagc gccaccatac agcagataca gcgcgtgcag
     2641 aaggacacaa atgatgacct tgctgcactt tacatgctga aggtacagaa aacaaaaaat
     2701 ggcataccct atgttgccgg tattggagcg gggattgagg atactgatgg ccagcccctg
     2761 agcaacatac tgctgctggc tgaccgtatt gcgatgatta acccggagga cggcaacacc
     2821 acgccgttat ttgtggcgca ggggaatcag ttgttcatga acgatgtgtt cctgaagcgg
     2881 ctgtttgcgg tgagtatcac ctcgtccggc aatcccccga cgttttccct gacgccggag
     2941 ggcaggctga ccgcaagaaa tgctgatatc agcggtaacg tgaatgcgaa ctccgggacg
     3001 ctcaacaacg tcacgattaa cgagaactgc cgggttctgg gaaaactgtc cgcgaaccag
     3061 attgaaggcg atctcgttaa aacagtgggt aaggcttttc cgcgggactc ccgggcaccg
     3121 gagcggtggc catcagggac cgttaccgtc aggatttatg acgatcagcc ttttgaccgg
     3181 caaattgtta ttccggcggt ggcattcagc ggcgctaagc atgagagaga gcatactgat
     3241 atttactcct catgccgtct gatagtgcgg aaaaacggtg ctgaaattta taaccgtacc
     3301 gcgctggata atacgctgat ttacagttgc gttattgata tgcctgccgg tcacggtcac
     3361 atgacgctgg agttttcggt atcagcatgg ctggtgaata actggtatcc cacagcaagt
     3421 atcagcgatt tgctggttgt ggtgatgaag aaagccaccg caggcatcag tatcagctga
     3481 attttataac ccatatacgg gcgccagaaa tggcgccttt tttattgcag aaaagcgaga
     3541 ggtaattatg cgtaaagttt gtgcagccat tttgtccgca gccatctgcc tgtccgtatc
     3601 cggtgcgcct gcatgggcgt ctgaacatca gtccacactg agcgcggggt atcttcatgc
     3661 ccgtacgaac gctcccggca gcgataatct gaacgggatt aacgtgaaat accgttatga
     3721 gtttacggac gcgctggggc tgattacgtc cttcagttat gccaatgctg aagatgagaa
     3781 aaaaacgcac tacagcgata cccgctggca tgaggattcc gttcgtaacc gctggttcag
     3841 cgtaatggcg gggccgtctg tgcgcgtgaa tgaatggttc agcgcgtatg cgatggcggg
     3901 tgtggcttac agccgtgtgt cgactttctc cggggattat ctccgcgtaa ctgacaacaa
     3961 ggggaaaacg cacga
//
LOCUS       DAJTMC010000244         3929 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.245, whole genome shotgun sequence.
ACCESSION   DAJTMC010000244 DAJTMC010000000
VERSION     DAJTMC010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3929
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.245"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            <1..93
                     /locus_tag="OO130_003991"
     CDS             <1..93
                     /locus_tag="OO130_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HCR5367195.1"
                     /translation="RLGRKSLSFSKSVELHDKVIGHYLNIKHYQ"
     gene            108..323
                     /locus_tag="OO130_003992"
                     /pseudo
     CDS             108..323
                     /locus_tag="OO130_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310610.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C"
     gene            305..1687
                     /gene="torZ"
                     /locus_tag="OO130_003993"
                     /pseudo
     CDS             305..1687
                     /gene="torZ"
                     /locus_tag="OO130_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707760.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
     gene            complement(1852..2823)
                     /gene="cmoB"
                     /locus_tag="OO130_003994"
     CDS             complement(1852..2823)
                     /gene="cmoB"
                     /locus_tag="OO130_003994"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HCR5367196.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRVVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(2820..3563)
                     /gene="cmoA"
                     /locus_tag="OO130_003995"
     CDS             complement(2820..3563)
                     /gene="cmoA"
                     /locus_tag="OO130_003995"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HCR5367197.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MISMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            complement(3604..>3929)
                     /locus_tag="OO130_003996"
     CDS             complement(3604..>3929)
                     /locus_tag="OO130_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HCR5367198.1"
                     /translation="RLQYRVAYGDGGFSELQSAIRIHGNAVEYIPIAIVLMLFMEMNG
                     AETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRSGMSATWCALLLMVLANLWYMPWEL
                     VFSLR"
BASE COUNT         1042 a          975 c         1038 g          874 t
ORIGIN      
        1 aggctgggac ggaagtcact gtcgttctca aaatcggtgg agctgcatga caaggtcatc
       61 gggcattatc tgaacataaa acactatcag taagttggag tcattaccgc ataggcgagc
      121 tggctaccgt gaatcctggt accgatctca ccgtcgttga tgccagtggc aaacagccga
      181 tcgttctgtt gcagggttat caaatgcagg gcagtgaaaa cacgctctac ctggcggcag
      241 gtcaacggct ggcgctagcc acattaagtg aagaaggtat caaggcgctc acgctaaacg
      301 gggaatggca ggctggggaa ttcagcgcca gcaatatggt gaacagaaac actggatgct
      361 ggtgacgctg gcggcggtgt tagggcagat tggtacaccg ggcggcggtt ttggtttctc
      421 ttatcattac tccaatggtg gcaacccgac acgtgttggc ggcgtgctgc cggaaatgtc
      481 tgcggcgatt gccggacagg ccagcgaggc cgctgatgat ggcggaatga gggcgattcc
      541 tgttgcccgt atcgtcgatg cgctggaaaa tcctggcggc aaatatcagc ataacggtaa
      601 agagcaaacg tatccgaata tcaaaatgat ctggtgggcg ggtggcggta actttaccca
      661 tcaccaggat actaatcgtc tgattaaagc atggcagaaa ccggagatga tcgtcgtttc
      721 tgaatgctac tggaccgcag ctgctaaaca tgcagatatc gtattaccga tcaccacatc
      781 gtttgagcgc aatgacttga cgatgaccgg tgattacagc aaccagcata ttgtgccgat
      841 gaagcaggct gtcgctccgc aatttgaagc gcgaaacgat tttgatgtgt ttgccgatct
      901 tgcggaatta ctgaaacctg gcggaaaaga gatctatacc gaaggtaaag atgaaatggc
      961 gtggctgaaa tttttctatg atgccgctca gaaaggtgcc cgtgcgcaac gcgtgactat
     1021 gccgatgttt aatgccttct ggcagcaaaa taaactgatc gaaatgcgcc gcagcgagaa
     1081 gaacgaacag tacgttcgtt atgctgattt ccgcgccgat ccggtgaaaa atgcgctggg
     1141 tacgccaagc ggcaaaattg agatttactc caaaacgctg gaaaaatttg gctataagga
     1201 ttgcccggca catccaacct ggcttgcgcc tgatgagtgg aagggtaccg ccgacgagaa
     1261 gcagttgcag cttctgaccg cacatccggc acaccgttta catagtcagc ttaactatgc
     1321 ggaactgcgt aaaaaatatg cggttgcaga tcgtgaacca atcactattc ataccgaaga
     1381 tgctgctcgc tttggtattg cgaatggtga tctggtgcgt gtgtggaaca aacgtggtca
     1441 gattctgaca ggcgcggtgg tgactgacgg gatcaaaaaa ggcgtggtat gcgtgcatga
     1501 aggtgcatgg ccagatctgg aaaatggctt gtgtaaaaac ggcagtgcga acgtgttaac
     1561 ggcggatatc cccagctcgc agctggcaaa tgcctgtgcc ggtaactctg cgctggtgta
     1621 tatcgaaaaa tatacgggta acgcaccgaa gttaacggca tttgataagc ctgctgttca
     1681 ggcgtaagaa gtgaatagcg cccggaaacg ggcgctgagg ttatgcaaac gtaaaactgc
     1741 ctgatgcgct atgcttatca ggcctacata gtccctgcaa tagattgaat tagcaagggt
     1801 ttgtaggccg gataaggcat tcacgctgca tccggcagta ataccagacc tttacggctt
     1861 gcgcgcaatc agcaccgcgc gtttaggcgc aggataacct tcaactgtct tactcggatc
     1921 atgtgggtcg aggaaatcgg acagagattc ggtaaccatc cattcagtgc gtcgctgctc
     1981 ttcagtagtg gtaacgcaca catccacaac gcggatatct acaaaaccac acttcttcag
     2041 ccagtttttc agcgccagcg cggaagggat gaaatagaca ttacgcattt gcgcgtaacg
     2101 atcgcctggt accagcaccg tgttctcatc gccatcaata accagcgttt ccagcaccag
     2161 ttcaccctca ttcaccagtt gatctttcaa ctgccagaga tgctccagcg gcgaacggcg
     2221 atggtaaagc acgcccatcg aaaagacggt atcaaaggct ttcagtgccg gaagctgttc
     2281 aatacctaac ggtaacaaat gtgcgcgctg atcgttaccc agtagtttac gcaccgcttc
     2341 aaactggcac agaaacagtt gcgtagggtc gatccccacc gcgaggtgcg cgcctgcgcc
     2401 aatcatgcgc cacatgtgat aaccgctgcc gcagccgaca tccagaatgg tgcgtccggt
     2461 taaatcagaa agatggggca gaacacgatc ccatttccag tcggaacgcc attcggtatc
     2521 gatgttgacg ccatacagtg agaacggccc tttgcgccat ggcatcaggt tccgcataag
     2581 cgtttcaatg cgtttaattt gcccggtgct cagtggctct tcgctttcgg cggttacgct
     2641 atgcaataaa tccagacgat acggtttaat ttcaggcaga aattccaccg cgttggacca
     2701 ctgcttaaac agcccgtgct gctgctcgcg ctgccagtta gcaatctgcg cgggcagcgt
     2761 ttcgagccag tgtgaaagat gatttttggc aatcagagaa taaaagttac caaagtcgat
     2821 catgcagcgt cctctgcttt taatgccacc agtgaaccaa agttaaagca ctggaaccac
     2881 agctcgctat gctcaaaacc ggctttatgc aggcgtgctt tatgggtttc cacggaatcg
     2941 gtcagcatca cgttttccag catgctgcgc ttctggctga tctccagttc gctgtaaccg
     3001 ttggcacgtt taaagtcgtg gtgcatgttg aacaacagtt caccaacttt ggcatcttcg
     3061 aaactgaatt tttccgaaag caccagcgcg ccgcccgggt tcagtccttg ataaatttta
     3121 tccagtaatg cctggcgctc ggaaggttcc aggaattgca gggtaaaatt cagcaccacc
     3181 atcgatgcgt tttcaatggc gatatcgcga atatcacctt caatgacgtc tactggcgta
     3241 ggggctttat aggcgtcaat atgacgacgg cagcgttcaa tcatcgccgg ggagttgtcg
     3301 atggcaataa ttttgcaatt atcatgatga atgttgcgac gcaccgagag cgtcgccgcg
     3361 cccagagaac aacccagatc gtaaacctgc gtaccaggtt gaacgaagcg ctcggctaac
     3421 atactaatca tggaaataat attggaatag ccgggaacgg aacgctggat catatccggg
     3481 aagacttcag ctacccgttc atcaaaggtc cagtcgccca gtctggcgat aggggcagaa
     3541 aatagcgtgt cgcggtgaga cataacgtaa aaatccggga aaaagaaagt ggcgtattgt
     3601 gcgctaacgc agggagaaaa ccaactccca gggcatatac caaagattcg ccagcaccat
     3661 cagcaacagc gcacaccagg tggcgctcat gccggaacgt cgccagcgaa acagacggtg
     3721 atgaaaaccg taataatgca tcagacgacc agcaagcaaa acgatgccgc aaatatgcac
     3781 catccaggtt tctgcgccat tcatttccat aaacagcatc agcacaatcg cgatgggaat
     3841 atattccacc gcgttaccat gaatgcgaat agcgctttgc agttcgctaa aaccaccgtc
     3901 accataggca acgcggtact gcaaacgca
//
LOCUS       DAJTMC010000245         3853 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.246, whole genome shotgun sequence.
ACCESSION   DAJTMC010000245 DAJTMC010000000
VERSION     DAJTMC010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3853
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.246"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            <1..439
                     /locus_tag="OO130_003997"
     CDS             <1..439
                     /locus_tag="OO130_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCR5367199.1"
                     /translation="FTTIMKENGLKANSLCVEITETVIERINEHFYLNIEQLRKQGVR
                     ISIDDFGTGLSNLKRFYEINPDSIKVDSQFTGDIFGTAGKIARIIFDLARYNRIPVIA
                     EGVESEDVARELIKLGCVQAQGYLYQKPMPFSAWDKSGKLVKE"
     gene            447..1775
                     /locus_tag="OO130_003998"
     CDS             447..1775
                     /locus_tag="OO130_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCR5367200.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLAMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVISGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGSYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKIYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(1822..3147)
                     /gene="rimO"
                     /locus_tag="OO130_003999"
     CDS             complement(1822..3147)
                     /gene="rimO"
                     /locus_tag="OO130_003999"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HCR5367201.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            3360..3743
                     /gene="bssR"
                     /locus_tag="OO130_004000"
     CDS             3360..3743
                     /gene="bssR"
                     /locus_tag="OO130_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HCR5367202.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
BASE COUNT         1006 a          858 c          951 g         1038 t
ORIGIN      
        1 ttttaccacc atcatgaaag aaaatggcct gaaggcgaac agcctctgtg ttgagatcac
       61 tgaaaccgtg atcgagcgaa ttaatgaaca tttttatctc aatattgaac aactgcgtaa
      121 acaaggggta cggatatcga ttgatgactt tggcaccggt ttgtcaaacc tgaaacgttt
      181 ttatgaaatt aatccagaca gcataaaggt ggactcgcaa tttaccggcg atattttcgg
      241 tactgcggga aaaattgcgc gcattatttt cgatctggca cgctataacc ggatcccggt
      301 gattgcggaa ggcgtagaga gcgaagacgt tgcacgcgaa ttaattaaat taggatgtgt
      361 tcaggctcag gggtatctgt accagaaacc tatgccattc tccgcctggg ataaaagtgg
      421 aaaattagta aaagagtagt ttacgtatgt ccagaatcaa taagttcgta cttacagtca
      481 gtctgctgat ttttatcatg atttcagcag ttgcctgcgg gatctacact caaatggtaa
      541 aggaacgggt gtatagcctg aaacagtccg ttattgatac tgcttttgcg gtggcaaata
      601 ttgctgaata tcggcgtagc gtggcaattg atcttatcaa cacgctaaat cccacggagg
      661 aacagctgtt ggttggtttg cgcacagctt acgccgactc ggtttccccc tcttatttgt
      721 acgatgtcgg tccttatctg atttccagtg acgaatgtat tcaggtaaag gagttcgaga
      781 aaaattattg tgcagatatt atgcaggttg tgaagtatcg acatgtcaaa aatacagggt
      841 ttatctcttt tgacggtaaa accttcgtct attacctcta tccggtaact cacaatcgta
      901 gtctgatatt tttgcttggt ctggagcgtt tttctttact gtcaaaatcg ctggcgatgg
      961 acagcgagaa cctgatgttc tctctattta agaacggtaa accggtgacc ggtgatgaat
     1021 ataatgctaa aaacgccatc ttcaccgttt cggaagcgat ggagcacttc gcctatttgc
     1081 cgaccggatt gtacgtattt gcgtataaaa aagatgttta tttgcgggtc tgtacattga
     1141 ttattttctt tgccgcattg gtggctgtga tatcgggtgc cagttgcctc tatctggtac
     1201 gcagagtgat taatcgtggt attgtggaga aagaagccat cattaataac cattttgaac
     1261 gcgtactgga tggcgggctt ttcttttcgg ctgccgatgt caaaaaactc tacagtatgt
     1321 ataactcggc gttcctggac gacctgacca aagcaatggg cagaaaatcc tttgacgaag
     1381 atttaaaagc gctgccggaa aaaggcagtt atttgtgcct gtttgacgtc gataaattca
     1441 aaaacattaa cgacaccttc ggtcatttgc tgggcgatga agtgttgatg aaagtggtga
     1501 aaatccttaa atcacagatc ccggtagata aaggtaaaat ctaccgcttc ggcggtgacg
     1561 aatttgcggt gatttatacg ggtggaacgc tggaagagtt gctatcgatt ctaaaagaaa
     1621 tcgttcattt ccaggtggga agcattaatt taagtaccag tatcggtgta gcgcattcaa
     1681 atgaatgtac caccgtcgaa cgcttgaaaa tgctggcgga tgagcggctg tataagagta
     1741 agaaaaacgg cagggctcag attagctggc agtaatcatt attcgcaggc cggacaaatg
     1801 attttgcccg gcctgaatta attaaacccg gctaccccac aaatcgtact catcggcgtg
     1861 ctcgactttc acacgcagga tatcacccgg cttaacgttg gtttcaccat tgagataaac
     1921 cgcgccgtcg atttccggtg catctgccat gctgcgacca atcgcgcctt cttcgtccac
     1981 ttcgtcgata atcaccagaa tttcacggcc cactttctct tgcaggcgct cggcggaaat
     2041 ctgctgctgc agctgcatga aacggttcca gcgttcttct ttaacttctt ccggaacctg
     2101 gtcaggcagg gcattggcgt ctgcaccttc aaccgggctg tatttaaagc agccaacgcg
     2161 atccagacgc gcttctttca ggaagtcgag tagcatctgg aaatcttctt ctgtctcgcc
     2221 agggaagccg acaataaagg ttgagcgtag ggtcagttcc gggcagattt cgcgccactg
     2281 tttgatgcgc gccagttggc gatctacaga acccggacgc ttcatcagtt tgagaatgcg
     2341 cgggctggcg tgctgcaacg gaatgtccag atacggcagg attttgcctt ctgccatcag
     2401 tgggatgacg tcgtccacat gcggataagg gtaaacgtag tgcagacgtg tccagatccc
     2461 cagtttcgat aactgttcgc acaggctgac catgctggtt tttaccggct cgccgttgtg
     2521 gaagccagta cgatgtttaa catcaacgcc ataggcggaa gtatcctgcg agatcaccag
     2581 aatctcttta acgcccgcat ctaccagacg tttcgcttca cttaacactt cgccaatcgg
     2641 acggctcacc aggtcgccgc gcatagacgg aataatgcag aaggtgcagc ggtgattaca
     2701 gccttccgaa attttcagat aggcataatg acgcggcgtc agtttcacac cttgttctgg
     2761 caccaggctc aggaatgggt tgtgtttcgg ttttggcacg tagtgatgaa cgtgctccag
     2821 aacctgctca tagctatgag gcccggtgat ttccagcact ttcgggtgga cttcgcggat
     2881 ctgatcttct tttgccccca gacaaccggt cacaattacc ttgccgtttt cattcaacgc
     2941 ttcaccaatg gcttccagtg attcttgtac cgcgctgtca ataaagccgc aggtgttgac
     3001 gatcaccatg tccgcatcgt catagctcgg taccacgtca taaccttcag tgcggagttc
     3061 ggtgagaata cgctctgaat cgacaaggtt tttcggacag ccaagggaaa caaagccgat
     3121 tttcggctgg ggagttactt tgctcatagc ttaaaaaata ttcagttaca ggaaaggtca
     3181 gggcagggat tctacagagt tctggataaa atttgtatcg caatctcatt cgctggcgga
     3241 ggcgaaggaa atgtaaattt tgttaattcg gcgtgaagaa ttgatcctgg acagcatttt
     3301 gctcaaaaaa tagccatact atttaattgc aacaaggctg gaaagaggag gatcgaagta
     3361 tgttcgttga cagacagcga atcgatctgc tgaaccggtt gatcgacgca cgcgttgacc
     3421 tcgctgcata cgtgcaactg aggaaggcaa aaggatacat gtccgtcagc gaaagcaatc
     3481 atctacgaga taactttttt aaactgaatc gcgaactgca cgataaatcg ctgcggttga
     3541 atcttcatct ggatcaggaa gagtggagtg ctcttcatca tgctgaagaa gcattagcga
     3601 cagccgcagt atgtttgatg agtgggcacc atgattgccc gactgttatt accgtcaacg
     3661 ccgataagct tgaaaattgt ctgatgagct taacgctgag tatccagagc ctgcagaagc
     3721 acgccatgct tgagaaggcc tgaaaactaa gggggagaaa gcgtctcccc cttcatgttt
     3781 aagtttttgt aaaaatgaat ttgttatctc ctccactgac tacgctttaa gccagagtca
     3841 atccggaggc gtt
//
LOCUS       DAJTMC010000246         3782 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.247, whole genome shotgun sequence.
ACCESSION   DAJTMC010000246 DAJTMC010000000
VERSION     DAJTMC010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3782
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.247"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(<1..209)
                     /locus_tag="OO130_004001"
     CDS             complement(<1..209)
                     /locus_tag="OO130_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HCR5367203.1"
                     /translation="MRHFIYQDEKSHKFWAVEQQGNELHISWGKVGTKGQSQIKSFSD
                     AAAAAKAELKLIAEKVKKGYVEQAK"
     gene            complement(350..2383)
                     /gene="metG"
                     /locus_tag="OO130_004002"
     CDS             complement(350..2383)
                     /gene="metG"
                     /locus_tag="OO130_004002"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HCR5367204.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQESRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMRQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            2515..3624
                     /gene="apbC"
                     /locus_tag="OO130_004003"
     CDS             2515..3624
                     /gene="apbC"
                     /locus_tag="OO130_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HCR5367205.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHCLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
BASE COUNT          906 a          970 c          970 g          936 t
ORIGIN      
        1 tccttcgctt gctccacata ccccttcttc accttctccg caatcagctt aagctccgct
       61 tttgccgctg ccgcagcatc tgaaaaactt tttatctggc tttgcccttt ggtgccaact
      121 tttccccaac tgatatgcaa ctcgtttccc tgttgctcaa ctgcccagaa tttatgtgat
      181 ttttcgtcct gatagataaa gtgtctcatt ctgcatccct gtaattttat ccagttgaat
      241 gcagatgcta ccagtattta tgcgggttag agagagacaa aatcgcaaga aaacaacttt
      301 aggaatttat aaagaaaagg cgctgtcgat gcagcgcctt gaagggggat tatttcacct
      361 gatgacccgg tttagcaccg gcatccgggc ttagcaggaa aatatctttc ccgccaggac
      421 cggcagccat caccatgcct tcagagatac cgaagcgcat tttacgtggt gctaggttag
      481 ccaccataat ggtgtgacga ccaatcagtg cctgcggatc cgggtaagca gaacgaatac
      541 cggagaagac attgcgtttt tcaccgccga gatccagcgt caggcgcagc agtttgtcag
      601 aaccttcaac aaactctgcg ttttcaatca gcgccacgcg caggtcaact ttagcgaagt
      661 cgtcaaaggt gatggtttcc tgaatcggat catctgccag cgggccagtt accggcgcgg
      721 cagcggcttt tacttcttct ttagaggctt ccaccagtgc ttcaacctgc ctcatatcga
      781 tgcggttata cagcgccttg aacggattca ctttgtggcc cagcagcggt tgctggatac
      841 catcccaggt cagttccgta ttgaggaatg cttctgcacg ctcggtcagt ttcggcagta
      901 ccggcttcag gtaagtcatc agcacgcgga acaggttgat gcccattgag caaattgcct
      961 gcaggtcggc atcgcggctt tcctgtttcg ccaccaccca cggagcctgt tcatcgacat
     1021 agcggttagc cagatcagcc agcgccatga tttcgcgcac ggctttacca aattcacggc
     1081 tttcccacgc ttcaccaatc acttcagcgg catcagtgaa ggttttgtac aactgcgggt
     1141 cagccagttc gcttgccagc acgccgtcaa aacgcttgtt gataaagccc gcattacggg
     1201 aggccaggtt aaccactttg ttaacgatat cggcattcac acgctgaacg aaatcttcca
     1261 ggttgagatc gatatcatcg atgcgcgaag agagtttcgc agtgtagtag taacgcaggc
     1321 tgtctgcgtc aaaatgattc agccaggtgc tggctttaat aaaggtgccg cgagacttgg
     1381 acatcttcgc gccgttcacc gtcacatagc catgaacaaa caggttggtc ggcttgcgga
     1441 agttgctgcc ttccagcatg gcaggccaga acaggctgtg gaagtaaacg atatctttac
     1501 cgatgaagtg gtacagctcg gcggtggagt ctttcttcca gtattcatcg aagcttacgc
     1561 tgtcgccgcg cttgtcgcac agattcttga aagaacccat gtagccaatc ggtgcgtcca
     1621 gccagacgta gaaatatttg cccggcgcgt tcggaatttc aaaaccgaag taaggtgcgt
     1681 cgcgggagat atcccactgt tgcaggccag attcaaacca ctcctgcatt ttgtttgcca
     1741 cctgctcctg caacgcaccg ctgcgggtcc atgcctgcaa catttcgctg aaagagggca
     1801 gatcaaagaa gaagtgttca gaatcacgca ttaccggggt agcgccagaa accaccgatt
     1861 tcggctcgat cagttcagtc gggctgtagg tcgcgccgca gacttcgcag ttatcgccgt
     1921 attgatccgg ggatttacat ttcgggcagg tgcctttcac aaaacggtcc ggcaggaaca
     1981 tgcctttttc cggatcgtac agctgagaga tggtgcggtt tttaataaaa ccgttttctt
     2041 tcaggcgaga gtagataagt tctgacaact ggcggttctc ttcgctgtgt gtcgagtgat
     2101 agttgtcata gctgatgtta aagcctgcga aatcagtctg atgctcctga ctcatttcgc
     2161 caatcatctg ctccggggtg ataccaagct gctgagcttt cagcatgatc ggtgtaccgt
     2221 gggcatcgtc ggcgcagatg aagttgacct cgtggccgcg cattcgctgg taacggaccc
     2281 agacatcagc ctggatgtgc tccagcatat ggccgaggtg gattgagccg ttagcgtacg
     2341 gcagtgcgca cgtcaccaga attttcttcg cgacttgagt catagtaggc attacttctt
     2401 aatagtgaaa aggggttaga tagtaccaaa tgggaaaatg ttaagtaagt acttctctat
     2461 attcttcagg taaactatca gctgatggtt tatctataca aaaaaggagt cgggatgaac
     2521 gaacaatccc aggccaaatc accagaagcc ttgcgcgcaa tggtcgccgg gacactggcc
     2581 aattttcagc acccaaccct gaagcataac ctcaccacgc tgaaagcgtt gcaccatgtc
     2641 gcctggatgg acgacacgtt gcatgtggaa ctggttatgc cattcgtctg gcatagcgcg
     2701 tttgaagagt taaaagagca atgtagcgcc gaactgctgc gtatcaccgg ggcgaaagct
     2761 atcgactgga aactgtcgca caatatcgcc accttaaaac gcgtgaagaa tcagccgggt
     2821 attaatggtg tgaagaacat cattgcggtc agttccggta aaggcggcgt gggtaaatcc
     2881 tccacggcgg taaacctggc gctggcactg gcggcggaag gggcgaaagt cggcattctg
     2941 gacgccgata tctacggccc atcaattccc accatgctgg gcgcagaaaa tcagcgtcca
     3001 acttcgccgg acggtaccca tatggcaccg attatgtctc attgcctggc aaccaattca
     3061 attggttatc tggtcactga cgacaatgcg atggtgtggc gtggaccgat ggcgagcaag
     3121 gcgttaatgc agatgttgca ggaaaccttg tggccggatc tcgactatct ggtgcttgat
     3181 atgccgccag gcaccggtga cattcaattg acgctggcgc aaaacattcc agtaactggt
     3241 gcagtcgtgg tcaccacccc gcaggacatc gcgttgatcg atgcgaaaaa aggcattgtg
     3301 atgttcgaga aagtcgaggt tccggtgctt ggtattgtcg agaacatgag tgtgcatatt
     3361 tgcagtaact gtggtcacca tgaaccgatt ttcggtaccg gtggtgcaga gaaactggct
     3421 gaaaaatacc acacacagct gctggggcag atgccgttac acatctcctt acgtgaggat
     3481 ctcgataaag gcacgccaac ggttatcagc cgtccggaga gcgaatttac cgctatctac
     3541 cgccagctgg ctgaccgcgt tgcagctcag ctctactggc agggtgaagt cattccaggc
     3601 gagatttcct tccgcgcggt ctaacgccta ttaaccattc tggttattct tcatacaccg
     3661 cagagatgtt acattgatgc ggtgtattag tttttgccgc attaatgaac acttatttaa
     3721 tactgcgaat attctgcgga ccttcgtccg aaactctaca aagtcgattg atcatacagg
     3781 ca
//
LOCUS       DAJTMC010000247         3759 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.250, whole genome shotgun sequence.
ACCESSION   DAJTMC010000247 DAJTMC010000000
VERSION     DAJTMC010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3759
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.250"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(<1..1412)
                     /gene="maeB"
                     /locus_tag="OO130_004004"
     CDS             complement(<1..1412)
                     /gene="maeB"
                     /locus_tag="OO130_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HCR5367206.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKP"
     gene            1701..2651
                     /gene="tal"
                     /locus_tag="OO130_004005"
     CDS             1701..2651
                     /gene="tal"
                     /locus_tag="OO130_004005"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCR5367207.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGISAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            2671..>3759
                     /locus_tag="OO130_004006"
     CDS             2671..>3759
                     /locus_tag="OO130_004006"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416960.1"
                     /note="catalyzes the formation of ribose 5-phosphate and
                     xylulose 5-phosphate from sedoheptulose 7-phosphate and
                     glyceraldehyde 3-phosphate; can transfer ketol groups
                     between several groups; in Escherichia coli there are two
                     tkt genes, tktA expressed during exponential growth and
                     the tktB during stationary phase; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCR5367208.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTGYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNT"
BASE COUNT          927 a          980 c          971 g          881 t
ORIGIN      
        1 atcggtttcg ccagtcccag cgttaccagt tcctgagtgg catgcagaac gcgcgcctct
       61 tccccttctg gcagaacaac gcgcttcggc gctttgcgag cctgggagaa aatcggcttc
      121 ataaacaggt tggttttgta aacgaactca gtcagcttgt cgatgtagac gtcgaaatca
      181 gcaatcggac gcgtcgccac gcccgactcc atcgcggctt tagcgaccgc aggagcgatc
      241 ttaacgatca agcgcggatc aaacggtttt ggaatgatgt attccggacc aaagctcaga
      301 tcctgatcgc catacgctga agccaccact tcgctctgtt ccgcatgggc gagttctgca
      361 atcgcacgta ccgccgccag tttcatctct tcgttgatgg cggttgcgcc aacgtccagc
      421 gcgccacgga agatgaacgg gaagcacagg acgttgttca cctggttcgg atagtcagaa
      481 cgaccggtgc aaatgatggc atccggacgc acttctttcg ccagcggcgg cagaatttcc
      541 ggttccgggt tcgccagcgc caggatcatt ggcgcacgag ccattttctt caccatttcc
      601 tgggtcagca ctttcgggcc ggaacatccg aggaaaatat ccgcgccttc aatcacatca
      661 tcgagggtac gtttgccgtc gtccaccacc gcatatgcgg ctttggtttc cgccatgttt
      721 ggctcacggc cctgatagat aacgcctttt gaatcgcaaa ccacgatgtt atgtttttgc
      781 agacccagcg ctaccagcag gttcatacag gcgattgctg cggcacccgc gccggaaacc
      841 accatccgca cgtcggagat gtttttctcc accacgcgca agccgttgag gatggcggca
      901 gtgctgatga ttgccgtgcc gtgctgatcg tcgtggaata ccggaatatt catccgctcg
      961 cgcagtttct gttcaatata gaaacattct ggcgctttaa tgtcttcgag gttgattccg
     1021 ccgaaggtcg gttcgagcgc agcgacaact tcaataaatt tgtccgggtc gagttcatca
     1081 acttcaatgt caaatacatc aatcccggcg aatttcttaa acagaacgcc cttgccttcc
     1141 atcaccggtt tgccagccag cgcgccaatg ttgcctaacc ccagcaccgc cgtaccgtta
     1201 gagatcaccg ccaccaggtt accgcgcgcg gtatatttgt aggcttttaa cgggtctttt
     1261 tcgatttcaa gacaaggtgc ggcaacgcct ggtgagtagg ccagcgccag atcgcgctgc
     1321 gttgccagag gcttggttgg agaaacctgg attttccctg gaactggaaa ttcatggaaa
     1381 tcaagtgcac tttgttttaa ctggtcatcc atttggttgt tcctttcacg taacgttcac
     1441 aaataaagtg tgtgggcaac agcccctgcc cacaacgtgg cgcacattat taccccgccg
     1501 gagtctacag actttgagca agtccaaact ctcaccatta atataatgtt ttggtaataa
     1561 tcctataaca ctgatgttac ctgcttaatc cagcaatacc atgcctgtct gctatgcttt
     1621 tttgatgcgt ttagcgaaat ttctcagaag tgtgaattaa cgcactcatc taacacttta
     1681 cttttcaagg agtatttcct atgaacgagt tagacggcat caaacagttc accactgtcg
     1741 tggcagacag cggcgatatt gagtccattc gccattatca tccccaggat gccaccacca
     1801 atccttcgct gttgctcaag gctgccggat tatcacaata tgagcattta atagacgatg
     1861 ctatcgcctg gggtaaaaaa aatggcaaga cccaggaaca acaggtggtc gcagcgtgtg
     1921 acaaactggc ggtcaatttc ggtgctgaaa ttctcaaaat cgtacccggt cgcgtgtcaa
     1981 cagaagttga tgcacgcctc tcttttgata aagaaaagag tattgagaag gcgcgccatc
     2041 tggtggactt gtatcagcaa caaggcgttg agaaatcacg cattctgatc aaactggctt
     2101 cgacctggga aggaattagc gcggcagaag agctggaaaa agaaggtatt aactgcaacc
     2161 tgacgctgct gttttctttt gcccaggcgc gggcctgtgc ggaagcaggc gtttttctga
     2221 tttcgccgtt tgtcgggcgt atttatgact ggtatcaggc acgcaagccg atggacccgt
     2281 atgtggtgga agaagatccg ggcgttaaat cggtgcgcaa tatctacgac tactataagc
     2341 aacaccacta tgaaaccatt gtgatgggcg cgagcttccg tcgcaccgaa caaatcctcg
     2401 ccttaaccgg ctgcgatcga ctgactatcg caccgaattt gctgaaggag ctgcaggaaa
     2461 aagtttcgcc agtggtacgt aaattaatcc caccttctca gacgttccca cgcccagctc
     2521 ccatgagcga agcggagttc cgttgggagc acaatcagga tgcgatggcg gtagaaaaac
     2581 tgtctgaagg cattcgtctg ttcgccgttg atcaacgcaa actggaagat cttcttgccg
     2641 ccaaactata aaccagccac ggagtgttat atgtcccgaa aagaccttgc caatgcgatt
     2701 cgcgcactca gtatggatgc ggtacaaaaa gccaactctg gtcatcccgg cgcgccgatg
     2761 ggcatggctg atattgccga agtgctgtgg aacgattttc ttaaacataa ccctaccgac
     2821 ccaaccgggt atgatcgcga ccgctttatt ctttccaacg gtcacgcgtc gatgctgctc
     2881 tacagtttgc tgcatctgac cggttacgac ctgccgctgg aagagctgaa gaacttccgt
     2941 cagttgcatt cgaaaacccc aggccacccg gagattggtt atacgcccgg tgttgaaacc
     3001 accaccggtc cacttggaca aggtttggcg aacgccgtcg gactggcgat agcagagcgt
     3061 acactggcgg cgcagtttaa tcagccggat catgaaattg tcgatcactt cacctatgtg
     3121 tttatgggcg acggctgcct gatggaaggt atttcccacg aagtctgttc gctggcgggc
     3181 acgctaggac tgggcaagct gattggtttt tacgatcaca acggtatttc gattgatgga
     3241 gaaaccgaag gctggtttac cgacgatacg gcaaaacgtt ttgaagccta tcactggcat
     3301 gtgatccatg aaatcgacgg tcacgatccg caggcggtga aggaagcgat ccttgaagcg
     3361 caaagcgtga aagataagcc gtcgctgatt atctgccgta cggtgattgg ctttggttcg
     3421 ccgaataaag caggtaagga agaggcgcac ggcgcaccac tgggggaaga agaagtggcg
     3481 ctggcacggc aaaaactggg ctggcaccat ccgccatttg agatccctaa agagatttat
     3541 cacgcctggg atgcccgtga aaaaggcgaa aaagcgcagc agagctggaa tgagaagttt
     3601 gccgcctata aaaaggctca tccgcaactg gcagaagagt ttacccgacg gatgagcggt
     3661 ggtttaccga aggactggga gaaaacgact cagaaatata tcaatgagtt acaggcaaat
     3721 ccggcgaaaa tcgctacccg taaggcttcg caaaatacg
//
LOCUS       DAJTMC010000248         3736 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.251, whole genome shotgun sequence.
ACCESSION   DAJTMC010000248 DAJTMC010000000
VERSION     DAJTMC010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3736
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.251"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(<1..859)
                     /locus_tag="OO130_004007"
     CDS             complement(<1..859)
                     /locus_tag="OO130_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HCR5367209.1"
                     /translation="MSSHFTPFTLKDISRPGGGFAMLAVDQREAMRLMFAAAGQPKPI
                     ADSVLTDFKVAATRILSPYASAVLADKQFCLEQIVEQGAVANSCGLIVAADLFIPGNG
                     IPVDSVEIDMSVDPHKAREMGAKAMKLLVLWREDEPAEERLAMVDKFVRRCRSAGLVS
                     IIEPVVRPPRRGWDFDRESAIVAAAAELGGTEADLYKAEMPLGGKGDEKTLLAACQQL
                     NDQMKMPWVILSSGVDADIFGRAVSIAMKGGASGFLAGRAVWASVVGAQDPQTMLRDV
                     SVPRLQRLAE"
     gene            complement(884..1762)
                     /gene="yihU"
                     /locus_tag="OO130_004008"
     CDS             complement(884..1762)
                     /gene="yihU"
                     /locus_tag="OO130_004008"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HCR5367210.1"
                     /translation="MATIAFLGLGQMGSPMANNLLQKGHSLQVFDVNAQAVDALVTQG
                     ATAAQTQAEAAAGAEFIITMLPNGDIVRQVLLGEKGVCEAVSSDALLIDMSTIHPLQT
                     DALIRELQEKGISMMDAPVGRTSVNAIDGTLLILAGGTDEQIARARPILMCMGNELVE
                     AGGPGMGIRVKLINNYMSIALNALSSEAAVLCESLGLNLDVAIKVMSGTAAGKGHFTT
                     TWPGKVLKGDLSPAFMVDLALKDLRIAVDVARETGTPLNMGIAAESYYAAASQDGKGR
                     QDWSALLNQVRQQAGL"
     gene            1935..2831
                     /locus_tag="OO130_004009"
     CDS             1935..2831
                     /locus_tag="OO130_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCR5367211.1"
                     /translation="MTRIACVGITVQDRIYSLPTLPEGGGKYQANHYLEIGGGPAATA
                     AVAIAKLGVEVDFIGRVGDDSCGNTLLAELEGWGVNTAFCRRYPNARSSQSAILVDQH
                     GERIIVNYPSPDLGTDAEWLEAIDFSRYNLILADVRWHSGTEKAFSLARLAGVTTLLD
                     ADMTPQDISPLVALADHAVFSTPGLKRMTGLQSPEEGLFQATTQTAGKVYVTLGSEGS
                     LWIEDGHLCQQEAFSVNVVDTTGAGDVFHGALAVALAEKMPTKEAIRFASAVAAMKCT
                     QPGGRAGIPNREQTESFLSLYA"
     gene            2871..3659
                     /locus_tag="OO130_004010"
     CDS             2871..3659
                     /locus_tag="OO130_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR5367212.1"
                     /translation="MSIIEVTGNPRHDQLVHLIAERGYMNIEELAQLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRVSSVMNTAFEQRELSLTAEKRAIAEAVADYLPERCTVFI
                     TIGTTVEAVARALLNRRDLRIITNSLRVAQILYKNQDIEVMVPGGTLRAHNGGIIGPG
                     AVDFIEGFRADYLITSIGAIEHDGTLLEFDLNEALVARTMIKHARNTLLVADHTKFAA
                     SAAVSIGNARNVRAFFTDAPPPNSFCQLLSEENVELVVAEQEVS"
BASE COUNT          961 a          955 c          902 g          918 t
ORIGIN      
        1 tttctgccaa ccgttgtaaa cgaggaacag agacatcgcg cagcatagtt tgtggatctt
       61 gtgcaccaac aaccgacgcc caaaccgctc gtccagccag gaagcccgac gcaccacctt
      121 tcatggcaat acttactgca cggccaaaaa tatcggcatc aacgccagaa gaaagaatta
      181 cccacggcat cttcatctga tcgttaagtt gctgacaggc ggcaagtaag gttttttcat
      241 cgcctttgcc ccctaatggc atctcggctt tgtaaagatc agcctctgtt ccaccaagct
      301 ctgcggcggc ggcaacaatg gcgctttcac gatcaaagtc ccaaccgcga cgaggtggac
      361 gcaccacagg ttcaataatg ctcaccagcc cggcactacg gcagcgacga acaaatttat
      421 caaccattgc cagacgttcc tctgcgggtt catcttcacg ccagagtaca agtaatttca
      481 ttgccttagc ccccatttca cgggctttat gcgggtcgac ggacatatct atttcaacgc
      541 tatcaaccgg gatgccatta cctgggataa aaagatcggc agcgacaatc aggccgcaag
      601 aattagcaac agcgccctgc tcgactattt gctcaagaca aaattgttta tccgccagaa
      661 cggcagaagc ataaggagaa aggattcggg tcgcggcaac tttaaaatcg gtgagtacac
      721 tgtcggcgat gggttttggc tgccctgctg ccgcaaacat caagcgcatg gcctcacgct
      781 gatcgacagc taacatggca aatccaccac ctgggcggga gatatctttc agcgtaaatg
      841 gggtgaagtg ggatgacata gaaactcctg ttaatagata aaattaaaga ccagcctgct
      901 ggcgaacctg atttagcagg gcactccagt cctgccgccc tttgccgtct tgacttgctg
      961 cagcgtaata actttctgct gcaatcccca tattgagcgg tgtccccgtc tcccgagcga
     1021 cgtcaactgc aatacgcaga tctttcaacg ccagatccac catgaaggcg ggtgaaagat
     1081 cgcctttcag cactttgcca ggccaggtgg tcgtaaaatg tcctttccct gctgccgtac
     1141 cactcatcac cttaattgcg acatcaagat ttaatcccag tgactcacat aacacagcgg
     1201 cctcggagga cagcgcatta agcgcaatgc tcatatagtt gttaattagc ttcacgcgga
     1261 tccccattcc tgggccgcca gcctcgacca gctcattacc catgcacata agaatcgggc
     1321 gtgcacgtgc aatctgctca tcagtgccac ccgctaaaat cagcaatgtg ccatcaatcg
     1381 cattcacgga cgttctgccc acgggagcgt ccatcatact gatgcctttt tcttgtaact
     1441 cccgtattaa tgcatcggtc tgtaagggat gaatagtcga catatcaatc aataacgcgt
     1501 cagatgaaac agcctcacag acgccttttt cccccaacag aacctgtcga acaatgtcac
     1561 cattgggaag catagtgatg ataaattcag cgcctgctgc tgcttccgct tgggtttgtg
     1621 ccgcagtggc cccctgcgta actagcgcat cgacggcctg tgcattgaca tcaaatacct
     1681 gcaatgaatg ccctttttgc aatagattgt tagccattgg gctacccatc tgccccagcc
     1741 cgagaaatgc aatggttgcc atgaatactc tcctgtctga cattgacaaa ataatgacaa
     1801 ttgaaaattc gtttttgtct gtttttgagc atatttgtga tttatgttca aaaacacgac
     1861 acgcatcact aatttgatca tttgcgtcat ttaaattgac caaagacgac aaaattaaac
     1921 aggacaagac aaatatgacc agaattgcat gcgtgggtat cacagttcag gacagaattt
     1981 attctttgcc cactctgccg gagggcggcg ggaaatatca ggcaaatcat tatcttgaga
     2041 ttggtggcgg cccggcagca acagccgctg ttgcaatcgc aaagcttggc gtggaggttg
     2101 atttcatcgg tcgcgtcggc gatgacagtt gtggaaatac cttacttgca gaacttgagg
     2161 gttggggcgt caataccgca ttttgccgcc gatatcctaa cgcccgttcg tcgcaatcag
     2221 cgatcctcgt tgatcagcat ggcgaacgga ttatcgttaa ttatccaagt cctgatctcg
     2281 gtactgatgc cgaatggtta gaagcgatcg atttctctcg ttataatctg attctggccg
     2341 atgttcgctg gcatagcggt actgaaaaag cattctcgct agcgcgcctt gcgggtgtga
     2401 caacgctttt agacgccgat atgacaccgc aggatatttc tccacttgtc gcgcttgccg
     2461 atcacgccgt gttctcaaca ccagggctga aacgcatgac tggcctgcag tctcctgaag
     2521 aaggtttgtt ccaggcaacg acacaaacag caggtaaagt gtatgttacg ctgggttctg
     2581 aaggatcgtt gtggattgaa gatggtcatt tgtgtcaaca ggaggcattt tctgtcaatg
     2641 tggttgatac cactggagca ggcgatgttt ttcacggcgc actcgcggta gcgttggcag
     2701 aaaaaatgcc aacaaaagag gctattcgtt ttgccagtgc ggttgccgcg atgaaatgta
     2761 cccagcctgg cggtcgggcc ggcattccta atcgtgagca aaccgaatca tttttgtcac
     2821 tttatgcgta aaatggtaaa catctaccga ggaggggcaa gggagtattc atgagtatca
     2881 tcgaagtgac aggtaatcca cgccatgacc aactcgttca tctaatcgca gaacgcggtt
     2941 atatgaatat tgaagaactg gcacaactgc tggatgtttc aacgcaaacc gtgcgccggg
     3001 atatccgaaa actcagtgag cagggcttga tcacccgtca tcatggaggg gccgggcgcg
     3061 tctccagcgt catgaatacc gcttttgagc aacgggaact ttcgctcacc gccgagaaac
     3121 gggcgatcgc tgaggcagtc gccgattacc ttcccgaacg ctgtaccgtc tttatcacca
     3181 tcggtacaac cgtagaagcc gttgccaggg cattactcaa ccggcgtgat ttacgcatta
     3241 tcactaacag cctacgtgtg gcacagattc tttataagaa tcaggatatt gaagtgatgg
     3301 tgccgggagg cactttacgc gctcataacg gcgggattat cggcccagga gccgtggatt
     3361 ttattgaagg cttccgcgca gattatttaa tcaccagtat aggtgccatt gaacatgacg
     3421 gcaccctact ggaatttgat ctaaatgaag cgttagtcgc gagaacgatg attaaacatg
     3481 cgcggaatac attgttagtg gccgatcata cgaagtttgc cgcgtctgct gccgtttcaa
     3541 ttggcaatgc acggaatgtc agggcttttt ttactgatgc cccgcctccc aattctttct
     3601 gccagttgtt aagtgaagag aatgttgaac tggtggttgc cgagcaagaa gtatcctgaa
     3661 attgaaattt cggaaaatcc gctgggaatt tcagcggatt ttgttttcca taagcaaaaa
     3721 aaacctgcca gcgatg
//
LOCUS       DAJTMC010000249         3729 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.252, whole genome shotgun sequence.
ACCESSION   DAJTMC010000249 DAJTMC010000000
VERSION     DAJTMC010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3729
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.252"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            1..329
                     /locus_tag="OO130_004011"
                     /pseudo
     CDS             1..329
                     /locus_tag="OO130_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001369610.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
     gene            446..595
                     /locus_tag="OO130_004012"
     CDS             446..595
                     /locus_tag="OO130_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001500869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5367213.1"
                     /translation="MSHYKVQQMLSCQKRRQKVQPVMQSGMQRPQQKKPGSQQKAHSQ
                     RNKAG"
     gene            628..1291
                     /locus_tag="OO130_004013"
                     /pseudo
     CDS             628..1291
                     /locus_tag="OO130_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000117976.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
     gene            1377..1889
                     /locus_tag="OO130_004014"
     CDS             1377..1889
                     /locus_tag="OO130_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5367214.1"
                     /translation="MGVTSGWVGSSAKSETGEQWMGAAGTKLGLSKPFMMSQMVGRTM
                     GCKIATEYYKWKSSDKVDNWGAVGADWPLEEKSKGTITNAASCGSGRLVGAVVTLSHF
                     LTNSTPTAAVYLAGGKAGNITVNVGGATQTMIYQGVVSGFQYYWSGSVSSAFVEAMKK
                     TGVPQDLKIS"
     gene            1965..2177
                     /locus_tag="OO130_004015"
     CDS             1965..2177
                     /locus_tag="OO130_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HCR5367215.1"
                     /translation="MPEKFFRTDADNNDVPMTAASWMALSEATEQAMFAKGVEINTRQ
                     LQMKAEVEALTDLKAIRSYVVGWPAG"
     gene            complement(2319..2644)
                     /locus_tag="OO130_004016"
                     /pseudo
     CDS             complement(2319..2644)
                     /locus_tag="OO130_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            complement(2780..3028)
                     /locus_tag="OO130_004017"
     CDS             complement(2780..3028)
                     /locus_tag="OO130_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HCR5367216.1"
                     /translation="MRIEICIAKEKMTKMPTGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
     gene            complement(3383..>3729)
                     /locus_tag="OO130_004018"
     CDS             complement(3383..>3729)
                     /locus_tag="OO130_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR5367217.1"
                     /translation="PAKVLPENWWQHPAALGATDSDIEIIKRQWGAFYGTDLELQLRR
                     RGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAASAEQHNNSINHIYPRIA
                     RVRSVEEILHAL"
BASE COUNT         1006 a          796 c         1037 g          890 t
ORIGIN      
        1 tcagaaaatc cggatgaagc cgggcgttac agtatggacg ttgagtatgg tcagtacagc
       61 gttattctgt tggtggaggg attcccgccg tcacatgccg ggactatcac cgtgtatgaa
      121 gattctcaac ccggtacgct gaatgatttt ctcggtgcca tgactgagga tgatgcccgt
      181 ccggaggcac tgcgtcgctt tgaactgatg gtggaagagg tggtgcgtaa cgcagaggag
      241 gcgaagaaga atgccggaga ggcggagacg tcagcgagga atgccggcat atcagccagt
      301 caggcagaag agagcgctgc aaatgctgac acttcagcag gggatgcatc ggagtcagcc
      361 cggcaggcgg cagaaagtgc agcctcagca aagcagtcag aggaggcgtc ctcgtcctcg
      421 gcctctgcgg ccgctcaaaa agccagtgag tcattacaaa gtgcaacaga tgctgagttg
      481 tcaaaaaaga cggcagaaag tgcagccggt aatgcagtca gggatgcaac gaccgcaaca
      541 gaaaaagccc gggagtcagc agaaagcgca cagtcagcgg aacaaagcag gatagcggcg
      601 gaagaggccg taaaccgaat ccccaccgtg gtgggacctc ccgggccaaa gggggaaccg
      661 gggcccgcgg gtcctcaggg gccgaaggga gataaaggag agcgtggcga caccggcccg
      721 gcaggggcca aaaggtgaca aaggcgatcc gggagtggct ggaccagcag gtccggcagg
      781 tgcgccgggg ccgaaaggcg ataaaggtga tccgggagta gcaggtccag caggtccggc
      841 ggggccacag ggaccgaagg gagacacagg agcccccggg caaggaacag aactgcttac
      901 tactgccaat acatggactc aggcacaaac ttttaatggt ggtattaatg gcaatttgac
      961 ggtgaccgga aacggctcat ttaacgatat tcagatccgt tcggataaac gcaacaagcg
     1021 aaatctggta aaactggata atgcgttaga tcgtctggag gcacttactg gttatcttta
     1081 cgagatacag tactctgccg acggttggca aacgtcggtt ggtttaattg ctcaggatgc
     1141 acaaaaagca ttgcctgaac tggtaactga agacgcagac gttatatctg gtgaaaaacg
     1201 tctgcgtctt aactacaacg gcataattgc attgttagtc gagggcttta aaacacttcg
     1261 tcatgagatt aaagaactcc gggagaagta aacgacagct gttgtagttt ctggtttcta
     1321 ctgaatttaa attgtggggt gacactcacc ccacgcattc agaaggggga gatgagatgg
     1381 gggtaacatc gggatgggta ggctcttcgg ctaagagcga aacaggtgag cagtggatgg
     1441 gagccgccgg aaccaaactg ggactgagta aaccttttat gatgagccaa atggttgggc
     1501 gaactatggg ctgtaaaatt gcaactgagt actataaatg gaaatcctct gacaaggttg
     1561 ataactgggg cgcagttggc gctgactggc ctttagaaga aaaaagtaaa ggtacaatta
     1621 caaacgccgc aagctgcgga tcagggaggc tggtaggggc tgtcgttaca ctttctcact
     1681 ttttgacgaa ctctacaccg acagctgctg tttatttagc cggtggtaaa gcaggtaaca
     1741 tcaccgtaaa cgtaggtggt gctacacaaa ccatgattta tcagggcgtt gttagtgggt
     1801 tccagtatta ctggtcaggt tctgttagtt ccgctttcgt ggaggcaatg aaaaagacgg
     1861 gagtacccca ggatctaaaa attagttaaa tggtaaataa ataattttaa aaacttcacg
     1921 ttctacatac cggagacgcc ggatatatag gatattgttt taagttgcca gagaaatttt
     1981 tccggacgga tgctgataat aatgatgtgc caatgacggc agcatcatgg atggcactga
     2041 gtgaagcgac agagcaggcg atgtttgcga agggagtgga gattaataca cggcagttgc
     2101 agatgaaagc cgaggttgag gcgctgacag acctgaaggc gatccgcagt tatgttgtcg
     2161 gatggcctgc tggctgaata aaaaaacggg accacgacca gtcccggaac catgagtttt
     2221 agggtattag tttgttatca tagttagcgt gctaagtatg ccatgtcagg ttgattagtg
     2281 aagtgatgtt gttcgcattt ttgcacggcg gagaatattc agatttttgg aaatccatat
     2341 ttttcccgtg cgcgattaca tgcttcattt acgatgctat tttcgccgga cattgcgaat
     2401 tccctgcatg tggacggttt ttgtaaacag agcaatatgc gttttctccg gggggggccg
     2461 gcaagagcgc cacatcgggg atttttctga ttggtgccgc gcatacagcg atgaaaagga
     2521 gatatttgct cagtgagact gaccgaaacc tttccgccag catcgtcagc ttctgcccag
     2581 taaaaagaga cgcggaaaat gcacaacagg caccacacgt catgcatgga ttcagattgc
     2641 tcataattca ccagtacagc tataaatcgt aaagagaaca gcggtacatc gtatgtaaga
     2701 acgtaccgcg gctggctggt gaacttccga tagtgcgagt attgaatgat ttccagccgt
     2761 taccgatttt acgtgctaat tagtgaacaa accactcgtc agcagactcc caggtatctt
     2821 tcagagtttc ctgaacaaaa gttttagctg aatctttatc ggcggtgcgc gtaacagaaa
     2881 ggccatcgtt gctggtagct tttacgatca cctctacatc gtcataacgt ttactgatgc
     2941 gtcgggttaa ttcttccttt aacgcatcca cagcaccggt tggcatttta gtcatttttt
     3001 ctttggctat gcagatttca atacgcataa aagtccctct acactgtgtt tgtatacagt
     3061 atcattttta actgtatgga taaacagtat taagggtgaa ggaaagtgat tttttggggc
     3121 atgcatgggg caaaaatatt tgttgtgggg cattttgggg catgattggg acactttgtt
     3181 acatatgaac tttgccgatt tttatatgac atggaaatac aacgctttga ttcttctaaa
     3241 taaaaaacat gctcttgggc gttctttagt gatttttaaa aattccgtat cacgcagtta
     3301 aagtggcggg catactcttc aaggctggtg atccccaacc gcacccattt aggatgcgac
     3361 cattgcggta gaccgatgta aatcataacg cgtgaaggat ctcttccacg ctacgcacac
     3421 gggcgatgcg cgggtagata tgattaatgc tgttattgtg ttgctcggcg ctggcggcac
     3481 tacaggcgtc ttcggcaatc accagattaa agccaagttc ccaggcattg cgggcggtgg
     3541 attcaacacc gatattggtc gagatcccac ataacactat tgtatcgata cctcggcggc
     3601 gcagttgcaa ctccagatcc gtaccgtaaa acgcacccca ctgacgtttg atgatttcga
     3661 tatcactgtc agttgcacct aatgcagctg gatgttgcca ccagttttcg ggcaacactt
     3721 tagcggggg
//
LOCUS       DAJTMC010000250         3723 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.253, whole genome shotgun sequence.
ACCESSION   DAJTMC010000250 DAJTMC010000000
VERSION     DAJTMC010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3723
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.253"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            37..264
                     /locus_tag="OO130_004019"
     CDS             37..264
                     /locus_tag="OO130_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HCR5367218.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDIHHGPYE"
     gene            complement(265..639)
                     /locus_tag="OO130_004020"
     CDS             complement(265..639)
                     /locus_tag="OO130_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HCR5367219.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGRAAADEWDER"
     gene            complement(722..1946)
                     /gene="mdtG"
                     /locus_tag="OO130_004021"
                     /pseudo
     CDS             complement(722..1946)
                     /gene="mdtG"
                     /locus_tag="OO130_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
     gene            complement(2118..3038)
                     /gene="lpxL"
                     /locus_tag="OO130_004022"
     CDS             complement(2118..3038)
                     /gene="lpxL"
                     /locus_tag="OO130_004022"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HCR5367220.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            3263..3723
                     /locus_tag="OO130_004023"
                     /pseudo
     CDS             3263..3723
                     /locus_tag="OO130_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415573.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
BASE COUNT          915 a          952 c          945 g          911 t
ORIGIN      
        1 cgttatgccg gtattttttt acggaagagg tatcaaatgc gtttaattgt ggtgagcatc
       61 atggtgacct tgctgagtgg ctgtggcagc attattagtc gcactatacc ggggcagggg
      121 catggcaacc aatattatcc cggtgtgcaa tgggatgtgc gtgactccgc ctggcgttat
      181 gtcacaatcc ttgatctgcc attctccctg gtttttgata ctttactgct gccgatcgac
      241 attcatcatg gcccgtatga gtgattaacg ttcatcccac tcatcagctg ctgcgcgtcc
      301 ttcctcggta tccagcggtg gctcaagctg aaattccccc tcgtcccatt catgtaatgt
      361 attctcttcc tgccactcct ggcgtatctc tatctcatca tagtcgccat caaaaacact
      421 ttgcgcggct tcaccgctaa gcataggtaa acattcacct tcttcccctt cgtcggcaaa
      481 aaactcaact tgccacatga tgtcgccgtc ctgcaaaacg tatttttggg cattgaactg
      541 ttgcacattc gcatcttcgg cgtcgatgcc ggggttgtct gcaagaaatt cttcgcgtgc
      601 agcgtcaatg gcttcttcaa gcgttgcgta catggtcatc acgattctcc ctttgagttg
      661 atgaggtttc agggaaaagg atagctgatt ctccgctttt gcaagtatga aaggcgaaaa
      721 atcagttcga tacctggggt attcgacgac gacgtagact gttccatgaa tagactgcgt
      781 tgaataacac tacgccagcg gtgacgagaa ataccgctct gaaaccgtag ttcgctgaaa
      841 tcgctgctcc catcaatggt ccggtaacgt tgccaatatc acgaaacgat tggttatagc
      901 tgaagatacg cccggcgatc tggttgctcg agttgtaaac caacaatgtc tgtacggcgg
      961 ggagtagtgc accatcggcg gcaccgagca aaaaacgtaa aatcccaagt tgcaatggcg
     1021 tctgaacgta agacattggg atcaacagca gtacagaaaa gatcagcgct gtaatcagga
     1081 tcttttcggg tccgattcga tcgccaagtt tgccgagtcg tggtgcactt agcagagccg
     1141 ccacgcctgg caccgaggcg atcatgccac tgataaaggc gacgttactg acgttacccg
     1201 ccagttcgcg gacatacagc gtcagaatgg gggcaattga gcccgtcgcc acctggatga
     1261 ttaacgtagt gacaaacagg ctgagtacca gtttcgggtt tttaagtgat gtcaccactt
     1321 cccgcatgtg cagcatctct tttttgctga ccggctggaa tttttctctg atgcaaaaca
     1381 gggtgacgaa aaagcagagt atgagcacac tggtggtaat aaagaatacc ggacgtaagc
     1441 cgtagctatc ggcgagcagg ccgccagcca ttgggccgag caacgcacca ccaacgccgc
     1501 ctgtggagag cgtacccagc gcccagccgc ttttattacg cggtacttgt gtggcgataa
     1561 gagcattagc gttggggaca aatccgccaa gtaacccaag aagcgcccgc aggatcaaaa
     1621 actgccagat attttgtgcc agccccatca acaccatcac gatgcccatg ccgagggcag
     1681 agcgtaatag catgagtttt cggcctttac ggtcggcgag tccaccccaa aacggtgagg
     1741 cgatggccga aaataaaaat gtaatgctga agacaatacc ggaccatatt cagggcggag
     1801 tgaccggtaa cgccaagctg ctcaacgtag aggggtaaga agggcattac cagactgaag
     1861 gcggcaccgg taagaaaaca gcctagccag gcgacgatca ggtttcgttt ccagtttata
     1921 ggggtgtcat tttcacaggg tgacatagca atccgctgtt ggtgcgccag gcgcggtgaa
     1981 cataagaaga aaagataagc acactaatta tgcgcccgac ttccaggggg cgcaatccag
     2041 agagctttta tcgctaaatc agggggattt gctgtggtaa tgccggatgc cattctgaag
     2101 catccggcag gggagattta atagcgtgaa ggaacgcctt ccgggcgtgt tttaaagcga
     2161 cggtgtaacc acatatactg ctctggtgcc atcatgatgc atttttcgac cactttgttc
     2221 atccacgcgg cggtagtttc ggcatcatcc agtggcggag aacactctgg cggcagcata
     2281 atcaattgat accctttgcc atctggctta cggcgtggaa cgaagggcac cagacatgcg
     2341 ccggacatcc gtgccagcat ccaggttccg gtcgtggtcg cagcctgctc aacggcaaat
     2401 aacgggacga aaacgcttga gcgcgggccg taatcatgat ccggtgcgta ccagaccact
     2461 tcgccttttt tcagggcttt aatcatgcct tttaaatctt tgcggtcgag catcgattta
     2521 tttgagcgca aacggcccca ggtttgtagc cagtcaatca gtggattatc gttcgggcgg
     2581 taaacgccaa tccccggttc ctgcatacca aactgccgcg cacccagctc cagtgtcaga
     2641 aaatggatgc caactaacag gatgccgcgt ttttgcgcct gcacgtcacg aatgtgttcc
     2701 atgccgatca cttccgtcca gcgcgcgatt cggcggtccg gccagaacca cgccatgccg
     2761 gtttccatca ggcccatgcc aacggattcg aaattcttca ccaccatttt acggcgttct
     2821 tgttcgctca tttccgggaa gcacagttcc aggttgcgat gcacaatttt tgcgcgtcgt
     2881 ttcataaaac gtaacgccag ttttcctaat ccacaaccga ggcggtagat aaccgggtag
     2941 ggcaattgca cgactaacca aagtacgcca atacccaacc aggttaacca ataacgcgga
     3001 tgaagcagtg cggtggagaa cttgggtaga ttcgtcatat caatcctgtt tttcaaccta
     3061 ttcgggcaat tgtatgtatt gtcgcatttt ttcgcccgca accaaaattt gtggctgaag
     3121 actgggcgaa attgccgcgc ttgtaaataa caaataattt ttaatgcgca aatgtagcgt
     3181 aaaatgtgtg gatgttaatt atcgataatt gctatatcat gccgcggatt tttactttcc
     3241 catctcgcag gaaccgtaca ccatgccagt gttacacaac cgcatttcca acgacgcgct
     3301 aaaagccaaa atgttggctg agagcgaacc gcgaaccacc atttcgtttt acaagtattt
     3361 ccacatcgcc gatcctaagg cgacccgtga cgctttatat cagctgttta ccgcgctgaa
     3421 tgtttttggg cgagtgtatc tggcgcatga gggcattaac gcgcaaatca gcgtacctgc
     3481 gagcaatgtt gaaacatttc gcgcgcagct ctgccttcga cccggcttta gagggtttac
     3541 gcctgaatat cgcgttggat gatgacggga aatccttctg ggtactgcgc atgaaggtac
     3601 gtgatcgcat tgttgccgac ggtattgacg atcctcactt tgatgccagc aatgttggtg
     3661 agtatctgca agcggcggaa gtgaacgcca tgcttgacga tcccgatgca ctatttatcg
     3721 aca
//
LOCUS       DAJTMC010000251         3676 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.254, whole genome shotgun sequence.
ACCESSION   DAJTMC010000251 DAJTMC010000000
VERSION     DAJTMC010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3676
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.254"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(118..2187)
                     /gene="glyS"
                     /locus_tag="OO130_004024"
     CDS             complement(118..2187)
                     /gene="glyS"
                     /locus_tag="OO130_004024"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HCR5367221.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLVESFAANFTVELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLDAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2197..3108)
                     /gene="glyQ"
                     /locus_tag="OO130_004025"
     CDS             complement(2197..3108)
                     /gene="glyQ"
                     /locus_tag="OO130_004025"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HCR5367222.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3203..3502)
                     /locus_tag="OO130_004026"
     CDS             complement(3203..3502)
                     /locus_tag="OO130_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HCR5367223.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
BASE COUNT          849 a         1000 c          947 g          880 t
ORIGIN      
        1 cataaaatta atatatgaga taatctttat tcagcagaag attattaaag gttgctgtat
       61 tatttagcga taataaaaaa agcctgccag atggcaggct ttttaataac ggcgttatta
      121 ctgcaacagc gaaatatccg caacgcgcag gaacagttcg cgcagtttct ccagcatggt
      181 cagacggttg agacgcaatt ctttgtcatc aaccatgacc atcactttat cgaagaaagc
      241 atcaaccggc tcacgcagct ctgccagttc gaccagcgca tcctggtaac gaccttccgc
      301 aaagtacggc tccagcttgt cacgtagcac cacaacctgc atcgccagtt taatttcttc
      361 cggctctttc agggtagagg cattcacgcg gtcgctcagc acttcgtcag atttcgccag
      421 aatgttagat acacgtttgt tcgccgccgc cagtgcagca gctgcatcca gggtacggaa
      481 atgcgatacc gctttcatac gggcatcgaa atcagccgga cgagtcggac gacgcgccag
      541 taccgcctgg atggtgtcaa cggtgtaacc ttcgtcctga taccaggcgc ggaagcgacc
      601 gagcataaag tcgataacat catcaaccac gttggcgtta gtcagcttat cgccatacag
      661 acgcactgct tcttcggtca gcgtttgcag atcaaggttg aggttcttct caacgataat
      721 tcgcagcacg ccaagtgcgg cacgacgcag cgcaaacggg tctttgtcgc ctttcggatg
      781 ctgaccgata ccgaagatac ccgccagagt atccatctta tcagcaatcg ccagcgcaca
      841 agctaccggg ttggacggca ggtcatcacc ggcaaagcgc ggctgatact gctcattcag
      901 cgcgacggct acgtcttccg cttcgccatc gtgacgcgcg tagtgcatcc ccataacgcc
      961 ttgggtgtcg gtgaactcga agaccatgtt ggtcatcagg tcacacttag acagcaggcc
     1021 cgcacgggtt gcgtggttaa cgtcagcgcc aatctgttca gcaatccagc cagccagcgc
     1081 ctggatgcgg tcagttttgt cacgcagcgt acccagctgt tgctggaaca gcacggtttg
     1141 caggcgcggc aggttatctt ccagacgttt tttacggtcg gtattgaaga agaactcggc
     1201 atccgccaga cgtggacgaa cgactttctc gttaccagag ataatttgct gcggatcttt
     1261 cgattcgatg ttggcaacga agatgaagtt cggcagcagt ttgccatcgt tcgcatacac
     1321 cgggaagtat ttctggtcac ctttcatggt gtaaaccagg gcttcagccg gcaccgcgag
     1381 gaatttctct tcgaattttg cggtcagaac gaccggccac tccaccagcg aagccacttc
     1441 ttccagcagg ctttcgctta agtcagcgtt accgccaatc ttacgcgctg cttcttcggc
     1501 atcggcttta atcttcgctt tacgttcttc gtaatcggcg atgactttcc cgcgctcacg
     1561 cagaatttcc ggatactgat cggcgttgtc gatggtgaat tccggttcgc ccataaagcg
     1621 gtggccgcga atcacgcgat cggactgaat gcccagaata gttgccggaa tgactttgtc
     1681 gcccagcagc agggtcacgg tgtgcaccgg acgcacgaag tgcacgtcgc ttgcgcccca
     1741 acgcatcagt ttcgggatcg gcagtttcgc cagagaagtc gcaaccatat tcggcagcag
     1801 tgcttcggtg ctttcgccct tcacatgggc gcgatacagc agccattcgc ctttatcggt
     1861 agtcagacgc tcagcctggt caacggtaat accgcaacca cgcgcccaac cttctgccgc
     1921 tttgctcggt ttgccttcag cgtcgaacgc ctgggcaatc gccgggccgc gtttttcgat
     1981 ttcacgatcc ggttgcgctt ccgccaggtt agctactttc agcgccagac gacgcggagc
     2041 agcaaaccat tgaacggtgc cgtgtgcgag gccagcgtta tccagctcca cagtaaaatt
     2101 cgcagcaaag gactcaacca ggctgcgcag tgcttttggt ggcagctctt cagtgccgat
     2161 ttccaccaga aaagttttct cagacatagc cgcctcttac ttatctttgt tgcacatcgg
     2221 gaagccgagg gcttcacggg aagcgtagta tgcttctgcc actgctttgg tcagggtgcg
     2281 aatgcgcaga atgtagcgct gacgctcggt gacggagatg gctttacgcg catccagcag
     2341 gttgaagctg tgggcggctt tcagaatacg ctcgtaggct ggcagcggca gcggattttc
     2401 cagcgccagc agctgctgcg cttctttctc gtactgctca aagcaggtga acaggaagtc
     2461 cacatccgcg tattcgaagt tgtaagtgga ctgctccact tcgttctgat ggaacacgtc
     2521 gccgtaggtg gttttaccca gcgggccgtc gctccagacc aggtcgtaaa cgctgtctac
     2581 gccctgaatg tacatggcca gacgttccag accgtaggtg atctcgccgg taaccggttt
     2641 acactccaga ccaccaacct gctggaagta agtgaactgc gtcacttcca tgccgtttag
     2701 ccacacttcc cagcccagtc cccaggcacc cagagtcggg ttttcccagt tatcttccac
     2761 gaaacggatg tcgtgaatag tcgggtccat gcccagctct ttcagagaac cgaggtacag
     2821 ctcctgaata ttgtccggcg atggcttaat gaccacctgg aactgatagt agtgctgtaa
     2881 acggttgggg ttttcgccgt agcgaccatc ggtcgggcga cgagaaggct gaacataagc
     2941 agccgccatc ggttctggcc ccagcgcgcg cagacaggtc attgggtgag aggttcccgc
     3001 gccgacttcc atgtccaatg gttgaacaat ggtgcagccc tggcgagccc agtaatcctg
     3061 taaggtcagg atcaagccct ggaaggtcct ggtatcaaac ttttgcatat tatttcgtgc
     3121 tggatacgtg tggatttaaa ggaagggatc agtatacccg ctggatggaa gatatacagt
     3181 acgaaacggg aaaaagcagg gcttaacgca tggaaagatg caaaaaatgg ccatctgcgt
     3241 caaaagaaca gacaaactgc tctttacgga aggtataacc gcgcatttca tagcttccct
     3301 ggaactgttc gaaggcggtg acgtcgattt tttgctcgcc ggtgttataa cgttttgccg
     3361 cctgatcctt gcataacgct tccatattca acgaacgcag aggatcaacc tttacccgtt
     3421 gtgctttctg cacgggtgac ggtgtagaac aacccactag caccataagc gccattgccg
     3481 gaaagaaagc gttcatcatc attaaattac cgtctggttt gcaggtgagt cttattattt
     3541 atatcggtat agtggctgat atcttacgaa tttccttaag cctctataag ctgtagtgat
     3601 aaaactcaga ttattccagt gcgccaacca aatgatttat cggtcagact ttttttaacc
     3661 agatacagag acacag
//
LOCUS       DAJTMC010000252         3676 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.255, whole genome shotgun sequence.
ACCESSION   DAJTMC010000252 DAJTMC010000000
VERSION     DAJTMC010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3676
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.255"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(167..481)
                     /gene="fliE"
                     /locus_tag="OO130_004027"
     CDS             complement(167..481)
                     /gene="fliE"
                     /locus_tag="OO130_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HCR5367224.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQDSLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            696..2354
                     /gene="fliF"
                     /locus_tag="OO130_004028"
     CDS             696..2354
                     /gene="fliF"
                     /locus_tag="OO130_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
                     /protein_id="HCR5367225.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYRFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     LSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESHAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEDLTREAMGFSEKRGDSLNVVNSPFNSSDESGGELPFWQQQAFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTSRAEAMKAVQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            2347..3342
                     /gene="fliG"
                     /locus_tag="OO130_004029"
     CDS             2347..3342
                     /gene="fliG"
                     /locus_tag="OO130_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HCR5367226.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     VATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            3335..>3676
                     /gene="fliH"
                     /locus_tag="OO130_004030"
     CDS             3335..>3676
                     /gene="fliH"
                     /locus_tag="OO130_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416450.2"
                     /note="binds to and inhibits the function of flagella
                     specific ATPase FliI; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HCR5367227.1"
                     /translation="MSDNLPWKTWTPDDLAPPQAEFVPMVEPEETIIEEADPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAKAKSQQAPIHARMQQLV
                     SEFQTTLDALDS"
BASE COUNT          925 a          966 c         1028 g          757 t
ORIGIN      
        1 ttcctatgag caaagcgaag acaaaacatg ttatcgcgtt tacggatcgt ggatggcaga
       61 aaactttcag gatcaggtat ccattttaaa ccagaaatag ggtgagtttg tcccatccat
      121 gccccacgcg ggcagatggg atgtaattac caacaggtta caaaacctac acctgcatgg
      181 acatcacttc ctgatacgcc gccaccagct tattacgcac ctgaatcccc atttgcatag
      241 aaactgaggc tttttgcata tcggtcatca catcgtttaa cgccacgccg ggttcaccga
      301 gggtgaattt ttctgcctgc gtgcgggcgg ctgtttgtgt atcgcttatg cgatcgagcg
      361 cggcgtgcag ctgcccggca aaactaatgg tcggttgcgg cagtgaatcc tgcgcacgcg
      421 cactcatcgc cgtagcctgt aactggctga taaccccttc aatcccctgt atcgctgaca
      481 ttttcgtctc ctggataatt tctggtagca aagcctacca gtaagtcaat aagacaaagg
      541 cgctaaatag caacaaaaaa acgggtttat tggcggatag aaaaaaacga aagcacaaat
      601 aatggcagcg tcaatttttc gagtttgctg acccgggagt gagtcttgtt ccactttgcc
      661 aataacgccg tccataatca gccacgaggt gcgcgatgaa tgcgactgca gcccagacaa
      721 aatctcttga gtggcttaat cgcctgcgtg cgaatccgaa aattccattg attgttgccg
      781 gttccgcggc agtggcggtc atggtcgcac tgatcctgtg ggcgaaagcc cccgactacc
      841 gcacattatt cagcaatctt tccgatcagg atggtggcgc aattgtcagc caactgacgc
      901 aaatgaatat tccttaccgc ttcagcgaag ccagcggcgc tattgaagtt ccggcagata
      961 aagttcacga gctgcgtctg cgcctggcgc aacaaggttt gcccaaaggc ggcgcggtcg
     1021 gtttcgaact actagatcag gaaaagtttg gtatcagcca gttcagcgaa caggtgaatt
     1081 atcagcgggc gctggaaggc gagctttctc gtaccatcga aactatcggc ccggtaaaag
     1141 gggcgcgcgt acatctggca atgccgaaac cgtctttatt cgtccgtgaa caaaaatccc
     1201 cttctgcatc ggtgacggta aatctattac ccggccgcgc actcgatgaa gggcaaatta
     1261 gcgccattgt acatctggtt tccagcgcgg ttgctggtct gccgccgggg aacgtcacgc
     1321 tggtggatca gggtggacat ctgttaaccc tgtccaatac cagcgggcgc gatcttaatg
     1381 acgctcagtt gaaatatgcc agcgatgtcg aaggccggat tcagcggcgt attgaagcga
     1441 tcctgtcgcc aattgttggt aacggtaata ttcacgccca ggtcacggcg caactggact
     1501 tcgccagtaa agaacaaacg gaagaacagt atcgccctaa cggtgatgaa tctcatgcgg
     1561 cgcttcgttc acgccagctt aatgagagcg agcaaagcgg ttccggttat ccgggcggcg
     1621 taccgggggc gttgtcgaat caaccggcac ctgcgaataa cgcgccaatc agcacgcctc
     1681 cggcaaatca aaataaccgc cagcagcagg cgagcaccac cagcaatagc gggccgcgta
     1741 gcacacagcg gaatgaaacc agtaactacg aagtcgatcg caccattcgt cataccaaaa
     1801 tgaacgtggg cgatgtgcaa cgtctgtcag tcgcggtagt ggtaaattac aaaaccttgc
     1861 cagacggtaa accattgcct ctcagcaacg aacagatgaa gcaaattgaa gatctgaccc
     1921 gcgaggcgat gggcttttct gaaaaacgcg gtgactcgct caatgtcgtt aactcgccgt
     1981 tcaatagcag tgacgaaagc ggcggagaac tgccgttctg gcaacagcaa gcgtttatcg
     2041 atcagttgct ggctgccggt cgctggttgc tggtactgct ggtggcgtgg ctgctgtggc
     2101 ggaaagcggt acgcccgcag ttaacaagtc gcgccgaggc gatgaaagct gtacagcaac
     2161 aggcgcaggc ccgtgaggaa gtggaagatg cggtggaagt ccgcctgagc aaagacgaac
     2221 aactccaaca acgacgcgct aaccaacgtc tgggggcaga agtcatgagc cagcgtatcc
     2281 gtgaaatgtc tgataacgat ccgcgcgtgg tggcgctggt cattcgccag tggataaata
     2341 acgatcatga gtaacctgac aggcaccgat aaaagcgtca tcctgctgat gaccattggc
     2401 gaagaccggg cggcagaggt gttcaagcac ctctcccagc gcgaagtgca aaccctgagc
     2461 gctgcaatgg cgaacgtcac gcagatctcc aacaagcagc taaccgatgt actggcggag
     2521 tttgagcaag aagctgaaca gtttgccgca ctgaatatca acgccaacga ttatctgcgt
     2581 tcggtattgg tcaaagctct gggtgaggaa cgtgccgcca gcctgctgga agatattctc
     2641 gaaactcgcg ataccgccag cggtattgaa acgctcaact ttatggagcc gcagagtgcc
     2701 gccgatctga ttcgcgatga gcatccgcaa attatcgcca ccattctggt gcatctgaag
     2761 cgcgcccagg ccgccgatat tctggcgttg ttcgatgaac gtctgcgcca cgacgtgatg
     2821 ttgcgtgtcg ccacctttgg cggcgtgcag ccagccgcgc tggcggaact gaccgaagta
     2881 ctgaatggct tgctcgacgg tcagaatctc aagcgcagca aaatgggcgg cgtgagaacg
     2941 gcagccgaaa ttatcaacct gatgaaaact cagcaggaag aagccgttat taccgccgtg
     3001 cgcgaattcg acggcgagct ggcgcaaaaa attatcgacg agatgttcct gttcgaaaat
     3061 ctggtggatg tcgacgatcg cagcattcag cgtctgttgc aggaagtgga ttccgaatcg
     3121 ctgttgatcg cgctgaaagg ggccgagcag ccactgcgcg agaaattcct gcgcaatatg
     3181 tcgcagcgtg ccgccgatat tctgcgcgac gatctcgcca accgtggtcc ggtgcgtctg
     3241 tcgcaggtgg aaaacgaaca gaaagcgatt ctgctgattg tgcgccgcct tgccgaaact
     3301 ggcgagatgg taattggcag cggcgaggat acctatgtct gataatctgc cgtggaaaac
     3361 ctggacgccg gacgatctcg cgccaccaca ggcagagttt gtgcccatgg tcgagccgga
     3421 agaaaccatc attgaagagg ccgaccccag ccttgagcag caactggcgc aactgcaaat
     3481 gcaggcccat gagcaaggtt atcaggcggg gattgccgaa ggtcgccagc aaggtcatga
     3541 gcagggctat caggaaggac tggcccaggg gctggagcaa ggtctggcaa aggcgaagtc
     3601 tcaacaagcg ccaattcatg cccggatgca gcaactggtc agcgaatttc aaactaccct
     3661 tgatgcgctt gatagt
//
LOCUS       DAJTMC010000253         3587 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.256, whole genome shotgun sequence.
ACCESSION   DAJTMC010000253 DAJTMC010000000
VERSION     DAJTMC010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3587
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.256"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(623..2332)
                     /locus_tag="OO130_004031"
     CDS             complement(623..2332)
                     /locus_tag="OO130_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HCR5367228.1"
                     /translation="MPLNKTFSSSIFSTKNSLSTDMSVNRDNRTITSSIMRVSNSSEL
                     IQFKNKTAPYFSEKRNVKVNINGVAKDIYGRQIVCRHLASYWEMNFMETNGKVNYQLL
                     STPDAIAKNVCLEKTEDFSKSPAYIYFVENKKWGTVITNFFYNMKKNGDFVRTLSACT
                     LNHQMALGLKIKRVQESEKWVVQFFDPNRTVTHKRTVFTCDSHFELSQLSAKDFFDDF
                     YWKIYGLEQPGQVIFEDRHNSPLTNTVKLLPDELINSRVIYHAITKNLTEVLFILMEK
                     YKNGEISQSKLVNLLATRSSDGTPAFYIALQNGCSDIIQVYGKILNMCNLSQETILTL
                     LAAVGANNVPGLCMSFMNGHVDTIKAYGEIVFKTPLTSDKRLYLLAAKDSHDLPGLFF
                     ALQNGHADSIRMFGSLLNKKMLSSEQIKELLKVKHGLFMALQNGHTKAIMAYGDILKI
                     LPPHQEYIDELLWIKNPNGTSGLFMAFYNGHTETIRAFCNILKNYSFTTRRLVEMLSA
                     TNKDGIPGVFVSVVNRDKETILEYCRIIKENNLEPDTIAEQFSKKMKKTFIEIINRFN
                     HFL"
     gene            complement(2764..3483)
                     /locus_tag="OO130_004032"
     CDS             complement(2764..3483)
                     /locus_tag="OO130_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector
                     phosphothreonine lyase"
                     /protein_id="HCR5367229.1"
                     /translation="MPIKKPCLKLNLDSLNVVRSEIPQMLSANERLKNNFNILYNQIR
                     QYPAYYFKVASNVPTYSDICQFFSVMYQGFQIVNHSGDVFIHACRENPQSKGDFVGDK
                     FHISIAREQVPLAFQILSGLLFSEDSPIDKWKITDMNRVSQQSRVGIGAQFTLYVKSD
                     QECSQYSALLLHKIRQFIMCLESNLLRSKIAPGEYPASDVRPEDWKYVSYRNELRSDR
                     DGSERQEQMLREEPFYRLMIE"
BASE COUNT         1066 a          720 c          639 g         1162 t
ORIGIN      
        1 aagcttgata caactggtct ggtatataca gtttctttga gtgcggttac gtccacagag
       61 gtaacggtaa aagtaacccc ttgtcgggac cactccgacc gcgcacagag gcattttcat
      121 cgctgacagg ccgcattgcc tcgcagggct ggaggaggat cggtgatatc cggacgaccg
      181 taccggctta cgccgggctt cggcggtttt actcctctgc catgtgcaac aacggagtgc
      241 cgccttacat gccgctggcg cggcatatcc tggtaacatt tttgttgata ccaccttccg
      301 gccttatcct gaccagtggg ctttcctggc atcgatgata ccgatgaacg cgcatgatat
      361 tgagccgacg atattacggg caactggaaa tactcaccct ctggatgtga cctttatcca
      421 tgaagaagat ttggccatac catggaaacc tgaacaatca tcagtttacg cccatgtaaa
      481 cccctacggg atatttgaac tcgatatgga aacaaggttg ccaatcgagg tggttgcctg
      541 aaagttggca gttacccggt cactacacct tgttggagtg aacatatcaa aaacaggtag
      601 ttacacaatt taaattacag tctcataaaa aatgattaaa tctatttatt atttcaataa
      661 acgttttttt catcttcttt gaaaattgtt ctgctatagt atccggttcc agattgttct
      721 cttttattat tctacaatac tctaatattg tttctttgtc tctatttact acagatacaa
      781 atacaccagg aataccatct ttattagttg cggacagcat ttcgaccaat cgtcttgtgg
      841 taaaactata gttttttaga atattacaaa aggcacgtat tgtttcggta tggccattat
      901 aaaatgccat aaaaagtcct gatgtaccgt tgggattttt tatccataat aattcatcta
      961 tatattcctg gtgagggggg agtattttta gtatatctcc atacgccata atggcctttg
     1021 tgtgcccatt ttgaagtgcc ataaacaacc catgctttac tttcaataat tctttaattt
     1081 gttcactact aagcattttc ttattaagca gacttccgaa catacgaata ctatctgcat
     1141 gtccattctg caatgcaaaa aataacccag gtaaatcatg gctgtctttc gcagctaata
     1201 gatataatct cttatcggaa gttaacgggg ttttaaagac tatttcccca taggctttta
     1261 ttgtgtctac atgtccgttc ataaaagaca tgcataaacc cggcacattg tttgccccta
     1321 ccgcagcaag caatgtaagt attgtttctt gactaagatt gcacatattt aatatcttac
     1381 catatacttg aataatgtct gaacatccgt tttgaagtgc tatataaaat gcaggagtac
     1441 catcagagct tctcgtggcc agaaggttca ccagtttaga ttgagaaatt tcaccatttt
     1501 tgtatttttc catcagtata aataatactt ctgtcagatt ctttgttatc gcatgatata
     1561 taactctgga attgatcaat tcatctggta aaagtttcac tgtgtttgtt agaggggaat
     1621 tatgcctatc ttcaaatatt acctgcccgg gttgctccag accataaatt ttccaataaa
     1681 aatcatcaaa aaaatcctta gcggataatt gactcagttc gaaatggcta tcacaagtaa
     1741 atacagtacg tttatgagta acagttcgat taggatcgaa aaactgaaca acccattttt
     1801 cactttcttg tacgcgcttt attttcaacc ctaacgccat ttggtggttt aatgtgcatg
     1861 ctgataatgt tctgacaaag tctccatttt ttttcatgtt ataaaaaaaa ttagtaataa
     1921 ctgttcccca ctttttattt tccacaaaat aaatataagc tggtgatttt gaaaaatctt
     1981 ccgttttttc caaacataca ttttttgcaa tagcatcagg ggtgctaaga agctgatagt
     2041 ttaccttccc atttgtttcc ataaaattca tttcccaata ggaagctagg tgacggcaaa
     2101 ctatttgtct tccatatata tcttttgcca ctccatttat atttaccttc acatttctct
     2161 tttctgaaaa ataaggtgct gttttattct taaactgaat tagttcactg gagtttgaga
     2221 ccctcataat agaacttgtt attgttctat tatctctgtt aacagacata tctgttgata
     2281 acgaattttt tgttgaaaat atactagatg aaaatgtttt atttaacggc atagaagaca
     2341 accatagaga tgttataata ctattagatt gcagttacta atagtaaaag cctccttaca
     2401 aggaggtagc ttattcagaa tgctcctata agatagcgag ctcgtcaacg atacagatga
     2461 tctgattata ttgtgcaaaa ggagaacagg aaaataggca ctggaaaaga tgaaggcctg
     2521 atgggagcat taactaatgc aaaatccgac aggttaatat cccgaaaagc aatcaaacga
     2581 ctcaaacagg agatgcacgc tgaagtggtg gacaactgtt cggatttcag atgagtagct
     2641 cagcagttta tgagccgaat gctctgtcac ggtcaggaat caggctgcat acgctgtgcc
     2701 aaatctaatc gtagttattc taaccagttt tccagctaca aggtggtgta gctggcatct
     2761 tctctactct atcatcaaac gataaaatgg ttcctctcgt aacatttgct cttgcctttc
     2821 acttccatct cgatcgcttc gtagttcatt acgatagctg acatatttcc agtcttcagg
     2881 tctaacatct gacgccggat actccccggg agcgattttg cttcttaata gattagactc
     2941 gagacacatt ataaattgtc gtattttatg aagtaataaa gcactatatt gcgagcattc
     3001 ctgatctgat tttacatata gcgtaaactg agctcctatc cccacacgag attgctgaga
     3061 aactcgattc atatcagtta tcttccattt atctatagga ctgtcctctg aaaataataa
     3121 accagacaaa atttggaatg ccagaggaac ttgctcccta gctatactaa tatgaaactt
     3181 gtccccaaca aaatccccct tactctgagg attttcacga catgcatgaa taaacacatc
     3241 tccactatgg ttcacaatct ggaatccttg gtacatgaca gaaaaaaatt gacatatatc
     3301 agagtaagtt ggcacgttcg atgctacttt gaaatagtac gctgggtatt gccgtatttg
     3361 gttgtacaat atattgaagt tatttttcaa tctttcattc gcagaaagca tctgcggtat
     3421 ttcagacctc acaacattca aagaatctaa atttagtttt agacagggct tttttatggg
     3481 catagaaaac gtcctctata aaatagatat attatcttat attgattgtt tatcatcttt
     3541 tataaaacga tacattatat gtaatgtatg taagcgtaag ggtcttc
//
LOCUS       DAJTMC010000254         3537 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.257, whole genome shotgun sequence.
ACCESSION   DAJTMC010000254 DAJTMC010000000
VERSION     DAJTMC010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3537
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.257"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            278..871
                     /gene="tetC"
                     /locus_tag="OO130_004033"
     CDS             278..871
                     /gene="tetC"
                     /locus_tag="OO130_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HCR5367230.1"
                     /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL
                     VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIID
                     NNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLN
                     GWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
     gene            complement(984..2189)
                     /gene="tet(B)"
                     /locus_tag="OO130_004034"
     CDS             complement(984..2189)
                     /gene="tet(B)"
                     /locus_tag="OO130_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HCR5367231.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            2271..2894
                     /gene="tetR(B)"
                     /locus_tag="OO130_004035"
     CDS             2271..2894
                     /gene="tetR(B)"
                     /locus_tag="OO130_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HCR5367232.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            complement(2872..3276)
                     /locus_tag="OO130_004036"
     CDS             complement(2872..3276)
                     /locus_tag="OO130_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000512977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HCR5367233.1"
                     /translation="MGISLNHGVHAKVSTPVHLRKARTCYDHLAGEVAVKIYDSLCQQ
                     QWITENGSMITLSGIQYFHEMGIDVPSKHSRKICCACLDWSERRFHLGGYVGAALFSL
                     YESKGWLTRHLGYREVTITEKGYAAFKTHFHI"
     gene            complement(3435..>3537)
                     /locus_tag="OO130_004037"
     CDS             complement(3435..>3537)
                     /locus_tag="OO130_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HCR5367234.1"
                     /translation="HLARLGRKSLSFSKSVELHDKVIGHYLNIKHYQ"
BASE COUNT         1187 a          734 c          657 g          959 t
ORIGIN      
        1 gccgctgaaa ataccacttg gtataacccg ctttattcgc cacatcatca agcagtaaag
       61 actgatctaa attatgttca atccataata gaacatcttt gataaccgtt gtatgaaact
      121 gcttatcatc atatcttaat tggatgttat tagctttatt ttgatagcga gaatgctgtt
      181 caatatacat aaaataacct aaatgttctt aagattgtca cgaccacatc atcatgatac
      241 cataaacata ctgacggtat gttattttaa atctatcatg gaaaataaaa atcatcaaca
      301 agaaaatttt aagagtacct atcaatcact ggttaactca gcacgaatat tgtttgttga
      361 aaaaggctat caagctgttt caatagatga gatctcggga aaagcgttgg tgaccaaagg
      421 tgccttttat catcacttta aaaataaaaa acaattactc agtgcctgtt ataagcagca
      481 attaattatg attgatgcct acatcacaac aaaaactgat ttaacaaatg gttggtctgc
      541 cttagaaagt atatttgaac attatcttga ttatattatt gataataata aaaaccttat
      601 ccctatccaa gaagtgatgc ctatcattgg ttggaatgaa cttgaaaaaa ttagccttga
      661 atacattact ggtaaggtaa acgccattgt cagcaaattg atccaagaga accaacttaa
      721 agcttatgat gatgatgtgc ttaaaaactt actcaatggc tggtttatgc atatcgcaat
      781 acatgcgaaa aacctaaaag agcttgccga taaaaaaggc caatttattg ctatttaccg
      841 cggcttttta ttgagcttga aagataaata aaatagatag gttttatttg aagctaaatc
      901 ttctttatcg taaaaaatgc cctcttgggt tatcaagagg gtcattatat ttcgcggaat
      961 aacatcattt ggtgacgaaa taactaagca cttgtctcct gtttactccc ctgagcttga
     1021 ggggttaaca tgaaggtcat cgatagcagg ataataatac agtaaaacgc taaaccaata
     1081 atccaaatcc agccatccca aattggtagt gaatgattat aaataacagc aaacagtaat
     1141 gggccaataa caccggttgc attggtaagg ctcaccaata atccctgtaa agcaccttgc
     1201 tgatgactct ttgtttggat agacatcact ccctgtaatg caggtaaagc gatcccacca
     1261 ccagccaata aaattaaaac agggaaaact aaccaacctt cagatataaa cgctaaaaag
     1321 gcaaatgcac tactatctgc aataaatccg agcagtactg ccgttttttc gccccattta
     1381 gtggctattc ttcctgccac aaaggcttgg aatactgagt gtaaaagacc aagacccgct
     1441 aatgaaaagc caaccatcat gctattccat ccaaaacgat tttcggtaaa tagcacccac
     1501 accgttgcgg gaatttggcc tatcaattgc gctgaaaaat aaataatcaa caaaatgggc
     1561 atcgttttaa ataaagtgat gtataccgaa ttcgattgcg tctcaacccc tacttcggta
     1621 tctgtattat cacgtgtatt tttggtttca cggaaccaaa acataaccac aaggaaagcg
     1681 acaatattta gcaacgcagc gataaaaaag ggactatgcg gtgaaatctc tcctgcaaaa
     1741 ccaccaataa taggccccgc tattaaacca agcccaaaac ttgcccctaa ccaaccgaac
     1801 cacttcacgc gttgagaagc tgaggtggta tcggcaatga ccgatgccgc gacagcccca
     1861 gtagctcctg tgatccctga aagcaaacgg cctaaataca gcatccaaag cgcacttgaa
     1921 aaagccagca ataagtaatc cagcgatgcg cctattaatg acaacaacag cactgggcgc
     1981 cgaccaaatc ggtcagacat ttttccaagc caaggagcaa agataacctg cattaacgca
     2041 taaagtgcaa gcaatacgcc aaagtggtta gcgatatctt ccgaagcaat aaattcacgt
     2101 aataacgttg gcaagactgg catgataagg ccaatcccca tggcatcgag taacgtaatt
     2161 accaatgcga tctttgtcga actattcatt tcacttttct ctatcactga tagggagtgg
     2221 taaaataact ctatcaatga tagagtgtca acaaaaatta ggaattaatg atgtctagat
     2281 tagataaaag taaagtgatt aacagcgcat tagagctgct taatgaggtc ggaatcgaag
     2341 gtttaacaac ccgtaaactc gcccagaagc taggtgtaga gcagcctaca ttgtattggc
     2401 atgtaaaaaa taagcgggct ttgctcgacg ccttagccat tgagatgtta gataggcacc
     2461 atactcactt ttgcccttta gaaggggaaa gctggcaaga ttttttacgt aataacgcta
     2521 aaagttttag atgtgcttta ctaagtcatc gcgatggagc aaaagtacat ttaggtacac
     2581 ggcctacaga aaaacagtat gaaactctcg aaaatcaatt agccttttta tgccaacaag
     2641 gtttttcact agagaatgca ttatatgcac tcagcgctgt ggggcatttt actttaggtt
     2701 gcgtattgga agatcaagag catcaagtcg ctaaagaaga aagggaaaca cctactactg
     2761 atagtatgcc gccattatta cgacaagcta tcgaattatt tgatcaccaa ggtgcagagc
     2821 cagccttctt attcggcctt gaattgatca tatgcggatt agaaaaacaa cttaaatgtg
     2881 aaagtgggtc ttaaaagcag cataaccttt ttccgtgatg gtaacttcac ggtaaccaag
     2941 atgtcgagtt aaccaccctt tagattcata aagcgaaaat aatgcggctc caacgtaccc
     3001 acctaaatgg aaacggcgtt cactccaatc taaacacgca caacagattt tacgtgaatg
     3061 tttggaagga acgtcaattc ccatttcatg aaaatattga ataccactta atgtgatcat
     3121 tgaaccattt tcagtgatcc attgctgttg acaaagggaa tcatagatct taacggcaac
     3181 ttcgccagct aaatgatcat agcaagtacg tgcttttcgt aaatgcactg gcgtggaaac
     3241 tttggcatgt acgccatggt ttaaggagat ccccatcata ctttccatca attcagcaat
     3301 atcttttcct gctagccgaa aataacgatg cttgccttga gctactactg tgattagctg
     3361 gcaatctaat aatttagata aatgactgct cgccgttgaa gctgatatat tcgccacagg
     3421 gtaatgactc caacttattg atagtgtttt atgttcagat aatgcccgat gactttgtca
     3481 tgcagctcca ccgattttga gaacgacagc gacttccgtc ccagccgtgc caggtgc
//
LOCUS       DAJTMC010000255         3529 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.258, whole genome shotgun sequence.
ACCESSION   DAJTMC010000255 DAJTMC010000000
VERSION     DAJTMC010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3529
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.258"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(293..988)
                     /locus_tag="OO130_004038"
     CDS             complement(293..988)
                     /locus_tag="OO130_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HCR5367235.1"
                     /translation="MDNRGEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(981..2408)
                     /locus_tag="OO130_004039"
     CDS             complement(981..2408)
                     /locus_tag="OO130_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HCR5367236.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVWFAKNKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(2419..3138)
                     /locus_tag="OO130_004040"
     CDS             complement(2419..3138)
                     /locus_tag="OO130_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HCR5367237.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVGHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
BASE COUNT          896 a          968 c          740 g          925 t
ORIGIN      
        1 gccttgtcaa aaaaatggta gatgaatacc aacaaaactc tgggcagtaa taaacaacat
       61 tctgatagag agtgcctgta taaagctgaa gtgacaatga ccgggaaggg aacccgtttt
      121 caggaatgga tgcctgtctt tttcactgta aatatcctgg cattactaat gccaggatat
      181 ttacagaaga gcagcataac catgacggaa cggggttaag tcaggttgtc cttaacgcta
      241 ttttcaacct cgtatgcctt cttcaggttt atgttcagat ctcatatctc tctcagcaat
      301 cctcaataat cagatacacc gctttcaccg ggccatgaac cccgacgact ttgataagct
      361 caatatccgc cgttgaacta gggccgctaa tgatgttaat gcaggacggc attcgttcac
      421 cggcctgcgc tttctgatgc aatttttctg cgagttgcgc tacacgcggc aggattgtgc
      481 ttttacgcag gataaaaaga gaagattccg ggagcaggct caatgaacgc ccgcgctcgg
      541 cggcggaaaa aagaaccacg cctcccgatt cggttaaacc atattcagca tacacaacac
      601 ccactttagc ctgctctgcc tgcgagatat tctcggcacc tttcgccgga tcccaaacaa
      661 cggcattgca ttcctgctgc aaacgttcac taatccccaa ttcctccagc ctcgtgtcac
      721 cgctaatcac gaccgactga tctcccagct ctttacacag acgtattgca gcttctgccg
      781 ccttcgcctc gctggtcagc tcacagcgcg tcaacataac atcgctggca aactgaataa
      841 acgcgtcaca gcgctgctgt tggttaagtt gggtaagccg ctcgttagca tagttgttaa
      901 gcggcgcatc ttctgcttgc ggttcaagtc gcagcgggcg acccagtgcc tgagcaacgt
      961 tattcaaaaa ttcgcctcga ttatccattc tttttctcct gcgcctgatg tttcttaaac
     1021 caactacgga aactctctcc gtcagcttca ggaagatcgc gtgcttccat ccagtcgcta
     1081 atcgcgccaa atttgagtgg tgttttgccg ccattgataa accagcttgc cgcatgagcg
     1141 ccggccatca tcccgacttt ccacaatcct ggatgactat tggcataagc gaacattttt
     1201 atcgcccgtt gctctgcttt tgcggtgatc cctttttcag ccatcacccg acgatgacgc
     1261 aaaatcagtt ttgacagcgg aatacgcacc ggacacacgc tgtcacaagc agtgcataaa
     1321 gagcaggcgt agggtaaatc tttaaaatct ttatagccgc caagtagcgg agaaatcacc
     1381 gcaccaattg gccctggata aatagagcca tatccatgac cgccaatatg gcgatatgcc
     1441 ggacaagtat tcatacaagc cccgcagcga atacagcgca gcacatcccg aaattcagag
     1501 gccagcacct cagaacgccc gttatcgaca ataaccagat gaaactcttc aggaccatca
     1561 acgtgccccg cttcgcgcgg tcctgtcagc caggtgttgt atcccgtcaa acgtgcacca
     1621 acggcactgc gcgccagcat ggtgatcaat acatctacct cggcaaacgt gggggcaata
     1681 cgctccattc ccatcactgc aatatgcgtt ttaggcagcg tggtacacat tcgcgcatta
     1741 ccttcattgg tcaccaggca taccgaacct gtctctgcca ccgcgaaatt acagccggta
     1801 atacctattt cagcactgag gaaatcttcg cggatttttt gccggatgaa taaggtcatc
     1861 gcttcaggcg tttccggccc ctcatagccc agacgttcgt gtagcactcg acggatctga
     1921 tggcgatctt tatgaattgc cgggaccaca acatgagatg gcggatcttg atccagctgg
     1981 agaatatatt cacccagatc ggtttcaatc acctgaatgc cagcatcctg caacacatga
     2041 ttgacaccaa tctcttcggt caccatcgat ttagatttca ccaccttccg ggcatttttg
     2101 cgttgggcaa cctgtaaaat gtagcgggta gcgtcttctt tgttttttgc aaaccagacg
     2161 tgaccgccgt tttgcgtcac tttttctgag agctggtaca gataagcgtc gagattactc
     2221 agaacatgat cacgtatctg ggcggcccga tcgcgccact cctcccagtg ccccaattca
     2281 tcgaccattt tttgccgatt tgccccaata cgctgctgcg cgtttgccac cgctttgcgc
     2341 atgatcggat cttcaatttg ctgacggatg cgagtcttaa aatctgtatt actggttttg
     2401 atcgacatct ttatatcctc agcggctcat caacacttca gcaatatgca tcactttgac
     2461 tttctgccct tcccgttgta atcgcccact gatgttcagc aggcaactca cgtcagcacc
     2521 aattaaatac tcagggcgga cttccatcag gtgcccaacc ttttctttca ccatctcgcc
     2581 ggatatttcg gccattttga ccgagaacgt gccgccaaat ccgcagcagg tatcctgttc
     2641 agcaaaggtc aacagctcca gtccacgcac atttttcagc agcgtaagtg gctcgtcctt
     2701 cactcccagc ttacgggcca ggctacaaga tgggtgatac acagctctcc cttgcaaact
     2761 ggcacctaca tcgactaccc ctaatttatt aacaataaaa gaggtgagat cctgcatacg
     2821 cgcggcaacc ttttcggcac gtgatgccca ttcaggttca tccgccagat acgtcgggta
     2881 actttttacg gcataggtgc aagagccagc cggtgaaata atgggatcgt cgttatcctc
     2941 cagtgcggcg atcagatttt tcatccctgg aatcgcttct ttgatataac cgctattgat
     3001 cgcaggctga ccgcagcatc cctgtttctc cgggaaattt acgcgacagc cgagtttttc
     3061 cagtagcagc acggagtctc gtgccattct tgatttcagg gcgtcaccaa tacaggtgac
     3121 aaagaaattg acattcacaa ctaatactcc attacttcat gcccatttat gcccgatgtt
     3181 tcccccccac cgggggatca atcatcaata aatatggcgt tcttttttaa ttggctagca
     3241 acatactacc cctcatttgt atgacaaatg ataatctttc acccaaacgt caaatgcaat
     3301 aactaatttt atttctttcg gcatcgctca cattttatgg agaaatcgac gatcattttt
     3361 ggcataagaa atccatttaa aaatggccac atcccttata cctattcaga gctacttatt
     3421 caggggttat tgagatatac actgatggtg tacaaacaaa atcaccgaca ataatcacgt
     3481 atcaagtaat agtcgggatt ttatttaatt gaagcaattt agtttgtat
//
LOCUS       DAJTMC010000256         3485 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.259, whole genome shotgun sequence.
ACCESSION   DAJTMC010000256 DAJTMC010000000
VERSION     DAJTMC010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3485
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.259"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            1..1946
                     /locus_tag="OO130_004041"
                     /pseudo
     CDS             1..1946
                     /locus_tag="OO130_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
     gene            complement(1989..2435)
                     /gene="ivy"
                     /locus_tag="OO130_004042"
     CDS             complement(1989..2435)
                     /gene="ivy"
                     /locus_tag="OO130_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HCR5367238.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            2616..3386
                     /gene="yafV"
                     /locus_tag="OO130_004043"
     CDS             2616..3386
                     /gene="yafV"
                     /locus_tag="OO130_004043"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HCR5367239.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAI
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMMALREYREKFPAWQDADEFRLR"
BASE COUNT          777 a          892 c         1000 g          816 t
ORIGIN      
        1 tgattatcaa aaaagagtac ggctgactgg agttctcagc ttatgcccag tctcgcgtgc
       61 tgcaaaaact ctccggcgtg agcgggatcc tggcgattac cgtcggcgtg ccgaactcat
      121 taggcccggg cgagctgctg caacattacg gcactgacga gcagaaagat cactatctgc
      181 cgcgtctggc gcgtggtcag gagatccctt gctttgcact gacaagcccg gaagcgggtt
      241 ccgatgcggg cgcgattccg gacaccggga ttgtctgcat gggcgaatgg cagggccagc
      301 aggtgctggg gatgcgtctg acttggaaca aacgctatat tacgctggca ccgattgcga
      361 ctgtgcttgg gctggcgttt aaactctccg acccggaaaa gctgctgggt ggcgcagaag
      421 atttaggtat tacctgtgcg ctgatcccga ccaccacgcc gggcgtggaa attggtcgtc
      481 gccacttccc gctgaacgta ccgttccaga acggaccgac gcgcggtaaa gatgtcttcg
      541 taccgatcga ttacatcatc ggtgggccga aaatggccgg gcaaggctgg cggatgctgg
      601 tggagtgcct ctcggtaggc cgcggcatca ccctgccttc caactcaacc ggcggcgtga
      661 aatcggtagc gctggcaacg ggcgcgtatg ctcacattcg ccgtcagttc aaaatctcta
      721 tcggaaagat ggaagggatt gaagagccgc tggcgcgtat tgccggtaat gcctacgtga
      781 tggatgctgc ggcatcgctg attacctacg gcattatgct cggcgaaaaa cctgccgtgc
      841 tgttggctat cgttaagtat cactgtaccc accgcgggca gcagtcgatt attgatgcga
      901 tggatatcac tggtggcaaa ggcattatgc tcgggcaaag caacttcctg gcgcgtgctt
      961 accagggcgc accgattgcc atcaccgttg aaggggctaa cattctgacc cgcagcatga
     1021 tgatcttcgg acaaggagcg attcgttgcc atccgtacgt gctggaagag atggaagcgg
     1081 cgaagaacaa tgacgtcaac gcgttcgata aactgttgtt caaacatatc ggtcacgtcg
     1141 gtagcaacaa agttcgcagc ttctggctgg gcctgacgcg cggtttaacc agcagcacgc
     1201 caaccggcga tgccactaaa cgctactatc agcacctgaa ccgcctgagc gccaacctcg
     1261 ccctgctttc tgatgtctcg atggcagtgc tgggcggcag cctgaaacgt cgcgagcgca
     1321 tctcggcccg tctgggggat attttaagcc agctctacct cgcctctgcc gtgctgaagc
     1381 gttatgacga cgaaggccgt aatgaagccg acctgccgct ggtgcactgg ggcgtacaag
     1441 atgcgctgta tcaggctgaa caggcgatgg atgatttact gcaaaacttc ccgaaccgcg
     1501 tggttgccgg gctgctgaat gtggtgatct tcccgaccgg acgtcattat ctggcacctt
     1561 ctgacaagct ggatcataaa gtggcgaaga ttttacaagt gccgaacgcc acccgttccc
     1621 gcattggtcg cggtcagtac ctgacgccga gcgagcataa tccggttggc ttgctggaag
     1681 aggcgctggt ggatgtgatt gccgccgacc caattcatca gcggatctgt aaagagctgg
     1741 gtaaaaacct gccgtttacc cgtctggatg aactggcgca caacgcgctg gcgaagggcc
     1801 tgattgataa agatgaagcc gctattctgg tgaaagctga agaaagccgt ctgcgcagta
     1861 ttaacgttga tgactttgat ccggaagagc tggcgacgaa gccggtaaag ttgccggaga
     1921 aagtgcggaa agttgaagcc gcgtaattga ataacggagc cgaaaggctc cgtttcttta
     1981 tccgctaatt atttaaaatt aaagccatcc ggatggtttt ccaggctgcc ggtcaacgcc
     2041 gcgaacagca ccgttttacc atcaatcgaa agcgcatcgt tcacattcag ccaggtgagt
     2101 ttctcttgcg acgttttctc atcaatagtc gagaacagcc ccgtcatctg attagatttc
     2161 tcggaccaca tcacagcgat acgttgcgag ccacagtcat gcggtttgca cgcgctcatc
     2221 acctgatacg tctcatctcc caacgttacg gtttgtgcgg gagtataagt accgcctttc
     2281 atcacccagg caggcagctt atgcccttgt accatctgat taaatgcagc tttggtggtt
     2341 tcgccctttg caaggctgct aatggttaaa tcatcctgcg ccattgcact ggtggcgatg
     2401 accagagcgg cgactgtcgt tattgcctta aacatcattc ctcccgagct tatcctgccc
     2461 atgttattaa ccttctaaag cctggcgata acacgataat ttgcaaatca gatatttcca
     2521 atatcagcat ttgttatcaa tttattctgc gctaaggcaa ctgcctgata aggtgttaaa
     2581 gttataaaac ccttcgcata aaggagttgt acaccgtgcc tggtttgaag attacgcttt
     2641 tgcagcaacc actggtgtgg atggatggcc ctgccaacct gcgtcatttt gatcgtcaac
     2701 tggaaggtat taccgggcgc gatgtgatcg ttctaccgga gatgtttacc agcggctttg
     2761 ccatggaagc ggcagcttcg tcgctagcac aagatgacgt agtgaactgg atgacagcta
     2821 aggcgcagca gtgcaatgcg ctgatagcag gcagtgttgc attacaaacg gagtctggtt
     2881 cggttaaccg ctttttgctg gttgagccgg gcggcacggt acatttttat gataagcgtc
     2941 atctgttccg catggcagat gagcatctac attataaagc gggcaatgcg cgagtgattg
     3001 tggaatggcg cggctggcgt attttgccgt tggtgtgcta cgacttacgt tttccggtgt
     3061 ggtcgcgcaa tctcaacgat tatgacctcg ccatctacgt tgccaactgg cctgctccgc
     3121 gctctctgca ctggcaggca ttgctgacgg cccgcgcgat tgagaatcag gcgtatgtgg
     3181 cgggatgcaa tcgcgtcggc agcgatggca acggctgcca ttatcgcggt gacagccggg
     3241 tgattaatcc acaaggcgag attatcgcta ctgccgacgc gcatcaggca acgcgcattg
     3301 atgcggagct gtcgatgatg gcattgcggg aatatagaga aaagtttccg gcatggcagg
     3361 atgcggatga gtttaggttg cggtgagtgg gggttgtgtt taaggctggg ggagagtcgg
     3421 ggtaagacta cgaaagcccg ctctccgcaa ggactgacgc cagatagttt ctgtccatag
     3481 ctgct
//
LOCUS       DAJTMC010000257         3409 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.261, whole genome shotgun sequence.
ACCESSION   DAJTMC010000257 DAJTMC010000000
VERSION     DAJTMC010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3409
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.261"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(19..321)
                     /locus_tag="OO130_004044"
     CDS             complement(19..321)
                     /locus_tag="OO130_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5367240.1"
                     /translation="MLEIVLSPVKAQQFTVTLDAQVCTIRLNQRTTGMYIDIIVNGEP
                     CLYGVLCLNNNRIVRYGYLPFQGDLFFTDTEGNSDPDWRGLGSRYRLYWLLPEELI"
     gene            complement(321..1031)
                     /locus_tag="OO130_004045"
     CDS             complement(321..1031)
                     /locus_tag="OO130_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000010340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5367241.1"
                     /translation="MSFSFDNISLNNFSLSESNVLSAVRGGGVLGLINSVLAPSFGIY
                     YARNDPAGAHQKGGKPFSPDSFVVVEVGAEASVSTVPVEQGAYTTFNKIQRSPELHVT
                     FIVEGWTAFSGSVPNLTNFSTTSRTNVLETLERMRTTAGLYDIETPDKTWTSCDLVKY
                     DYRMRSNNGPTLLTVTAVFQTVMNTGEVSVGSTDKSSPTDNDKAKGAASVKTQPVTAS
                     VTQPSDADRRSATNRGIT"
     gene            complement(1028..3049)
                     /locus_tag="OO130_004046"
     CDS             complement(1028..3049)
                     /locus_tag="OO130_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HCR5367242.1"
                     /translation="MAQVLDELVLSLGIDDRDFTAGEQAVHAALNRLTTVMEGVADTF
                     SQGQKKTSESLEKTGKDADKTARRMEDAGKRASRFFSGIRSEILALAGVSLTLGGLKN
                     LVTGFARDLNRLSVESDAFGMKARNLDGWLRAARANNVDEGEMSGAFSRLANAKAAFR
                     AGRSFDPVLQDLFQVAARAGISVDLNTDSTEAIMRKLAVIFPRLTKSEQTAYGNALGF
                     SYAGQQFLGSGHALKDVDEFTSRSQVTPGRTALARKLRKELVELDQTWSGIGMTISTA
                     LMPYALKLSHWLETLGDWMQQHPEEVNRFITTFLNDVESVASLANKAAGELGGWQNVI
                     ITLIGLKVASWVRGLTKALSGPGGLLFAITALYPVVDGLLTSIVGRENKDWLDSHGFF
                     WASDGTFFFNKKEMETYQTKLDAGEKPGNITRAQSPTVWQQGMLDTQASQATGREAAF
                     GESWLQGMRATQEKLGNAMQNRQRPTKTGEALLGWLQPKLSQLEEKYNLPPGLLRSVA
                     ITESRGNQFAVSPAGAMGLFQFMPKTASEFGLRGNDAFDPEKAADAAARKLGGLIRFF
                     HGDLAKALAAYNWGEGNVQRKGLAAAPEETRNYIPRVLANLPYPGAAMAIRSLHPAPV
                     SQSTVTETTHIGTLNVTTTSDNVKGITDDARRRITNSALVSVYSSGVTG"
     gene            complement(3033..3215)
                     /locus_tag="OO130_004047"
     CDS             complement(3033..3215)
                     /locus_tag="OO130_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HCR5367243.1"
                     /translation="MVDYVNVPRTIATVISSGKASKAELDSVLGVQDLWDLLEIIHVD
                     AHNEQVIQENRNGAGT"
BASE COUNT          796 a          992 c          816 g          805 t
ORIGIN      
        1 ttttacgttg tctgtagctc atatcagttc ctcaggtaac agccagtaga gccggtaccg
       61 ggatccgagc ccccgccagt cagggtcact attcccctcc gtgtcggtaa aaaacaggtc
      121 cccctgaaac ggcaggtatc cgtaccggac aatccggtta ttattcagac acagcacgcc
      181 atacaggcac ggttcaccat taacaatgat atcgatatac atccccgtag tacgctgatt
      241 caggcgaatg gtgcagacct gagcgtccag tgtcaccgta aactgctggg ctttaacggg
      301 agataaaaca atttccagca tcaggtgatc cccctgttcg tggcacttcg tctgtcagcg
      361 tctgacggtt gtgtcaccga cgccgtaact ggctgcgttt taaccgatgc ggcccctttt
      421 gctttatcgt tgtccgtggg agacgactta tccgtacttc ccactgacac ctctcctgta
      481 ttcattacgg tctgaaatac cgcagtaacc gtcagtaagg tcggaccatt attacttcgc
      541 attcggtagt catatttcac aaggtcgcag gatgtccatg tcttgtccgg cgtctcaata
      601 tcgtagagtc ctgccgtggt acgcatccgt tcaagcgttt ccagcacatt cgtccgcgag
      661 gtggtggaaa aatttgtcag gttcgggacg gatccggaaa aggccgtcca cccctctaca
      721 atgaaagtca catgcagttc cggcgatctc tggattttat taaaggtggt ataagccccc
      781 tgctccacgg ggacggtgga aacagaagcc tccgcgccca cctcaacgac aacaaaagaa
      841 tccggggaga aaggtttgcc acccttctga tgggcacctg ccggatcatt ccgtgcgtaa
      901 taaataccga atgacggtgc cagtacacta ttaatgagtc ccaggacacc gccgccacga
      961 actgcactca gtacgttact ttcactgagc gaaaagttat tcagggaaat attatcgaaa
     1021 gaaaaactca tcctgttacc ccgctggaat aaaccgacac aagcgccgaa ttcgtgatac
     1081 gccgacgtgc gtcatcggta atacccttca cattgtccga cgttgtggtg acattcagtg
     1141 tcccgatatg cgtggtttcc gttacggtgg actgcgatac aggcgcagga tggagcgacc
     1201 gtatggccat tgccgcaccc ggataaggca ggttcgccag cacccgcgga atatagttgc
     1261 gggtctcctc cggagcagca gccagtccct tacgctgaac atttccctct ccccagttat
     1321 atgccgccag agccttagcc agatcgccat gaaaaaaacg tatcaggcca ccaagttttc
     1381 tcgcggcggc atcagcggct ttttcgggat caaaggcatc gttccccctc agaccaaatt
     1441 cactggccgt cttcggcatg aactgaaaca gtcccatcgc accagcaggt gagacggcaa
     1501 actgattacc acgggattcg gtgatcgcaa cgctgcgcag cagtccgggc ggcaggttat
     1561 atttttcctc cagttgagac agtttcggtt gcagccagcc taacagggct tccccggtct
     1621 tcgtcggtcg ctggcggttt tgcatggcat taccgagttt ttcctgcgtc gcacgcatac
     1681 cctgtagcca ggattcaccg aaggctgctt ccctcccggt tgcctgagaa gcctgagtat
     1741 ccagcatgcc ctgctgccat actgtgggtg attgtgcccg ggtgatgttg ccaggctttt
     1801 ctccggcatc cagttttgtc tgatacgtct ccatctcttt cttattgaaa aagaaagtcc
     1861 cgtctgaagc ccagaaaaaa ccatgtgaat ccagccagtc cttattttcc ctgccaacaa
     1921 tggatgtcag taatccgtca acaaccgggt aaagcgccgt tatcgcaaaa agaaggcctc
     1981 cggggccact gagggctttt gtcagccccc gcacccatga cgccactttc agcccgatca
     2041 gcgtaataat gacattctgc cagcccccca gttctccggc agccttattc gccagggagg
     2101 ccactgactc aacgtcattc agaaatgtgg tgataaacct gttcacttcc tccggatgtt
     2161 gctgcatcca gtcaccgagt gtctccagcc agtggctgag cttcagcgcg tacggcatca
     2221 acgcggtgct aatcgtcatc ccgatgccgg accaggtctg atcgagctca acaagctctt
     2281 ttcgtagctt acgggccagc gctgtacgcc ccggcgtaac ctgcgaacgg gaagtaaatt
     2341 cgtccacatc tttcagcgca tgaccggagc caagaaactg ctgtccggca taactgaagc
     2401 ccagcgcgtt gccgtaggca gtttgttcgg actttgtcag tcgcggaaaa ataaccgcca
     2461 gcttgcgcat gatggcttca gtgctgtcag tatttaaatc aacactaatg cccgccctgg
     2521 ctgcaacctg aaacaaatcc tgtaacacag gatcaaagga ccttccggct ctgaaggccg
     2581 cttttgcatt tgcaagccgg gaaaatgccc cagacatttc cccttcatca acattatttg
     2641 cccgcgcagc ccgtaaccat ccgtccagat ttctggcctt catgccaaaa gcatcggatt
     2701 ccacggacag acggttcaga tcacgggcaa aacctgttac caggttcttc agccccccca
     2761 gcgtcagact gacacccgca agagccagaa tctcgctacg gatccctgaa aaaaaacggg
     2821 acgcccgttt cccggcatct tccattcgac gggccgtttt atcagcatct tttccggttt
     2881 tttccagtga ttcactggtt ttcttctgtc cctgactgaa tgtgtcagcg acaccttcca
     2941 tcaccgtcgt gagccggtta agggcagcat gtactgcctg ttccccggca gtaaagtccc
     3001 tgtcatcaat cccgagggac agaacaagct catcaagtac ctgcgccatt tctgttctcc
     3061 tgtatcacct gttcgttatg ggcatccaca tgaattattt ccagcagatc ccataagtcc
     3121 tgcacaccaa ggacagaatc cagttcagct tttgaagcct taccggagga gataacggtc
     3181 gcaatggtac ggggaacatt aacgtaatcc accaccccga acggtctgtc ggggtcgaga
     3241 taacgcgggg ggatatctag ctgacgacgg tagcgaaaaa atccacatgc agcttaaaga
     3301 cctccgcacg cagattaaga cgggtaatga tttcttcaat atcctcttca acaagcggtc
     3361 tccggatgct gcgttttttc ggatcgggaa caaactgcac acagtccat
//
LOCUS       DAJTMC010000258         3364 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.262, whole genome shotgun sequence.
ACCESSION   DAJTMC010000258 DAJTMC010000000
VERSION     DAJTMC010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3364
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.262"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(117..1571)
                     /gene="ospC2"
                     /locus_tag="OO130_004048"
     CDS             complement(117..1571)
                     /gene="ospC2"
                     /locus_tag="OO130_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC2"
                     /protein_id="HCR5367244.1"
                     /translation="MKIPEAVNHINVQNNIDLVDGKINPNKDTKALQKNISCVTNSSS
                     SGISEKHLDHCADTVKSFLRKSIAAQSYSKMFSQGTSFKSLNLSIEAPSGARSSFRSL
                     EHLDKVSRHYLSEIIQKTHPLSSDERHLLSIIINSDFNFRHQSNANLSNNTLNIKSFD
                     KIKSENIQTYKNTFSEDIEEIANHDFVFFGVEISNHQETLPLNKTHHTVDFGANAYII
                     DHDSPYGYMTLTDHFDNAIPPVFYHEHQSFFLDNFKEVVDEVSRYVHGNQGKTDVPIF
                     NTKDMRLGIGLHLIDFIRKSKDQRFREFCYNKNIDPVSLDRIINFVFQLEYHIPRMLS
                     TDNFKKIRLRDISLEDAIKASNYEEINNKVTDKKMAHQALAYSLGNAKSDMALYLLSK
                     FNFTKQDIAEMEKMNNNMYCELYDVEYLLSEDSANYKVLEYFINNGLVDVNKRFQKAN
                     SGDTMLDNAMKSKDSKTIDFLLRNGAVSGKRFGR"
     gene            1996..2840
                     /locus_tag="OO130_004049"
     CDS             join(1996..2338,2338..2840)
                     /locus_tag="OO130_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HCR5367245.1"
                     /translation="MARYDLPDEAWTIIKPLLPPEPATPRAGRPWAEHRKIINGMFWV
                     LCSGAPWRDLPERYGAWKTVYNRFNRWSKSGVINIIFNRLLSLLDANGFIDWSATALD
                     GSNIRALKCAAGGSKKHPDIDGDNGPGRSRGGFGTKIHLATDGSGLPLNIVLSPGQAH
                     ESQFAQRLLDGIGVQRQNGSMKRRGHAVLADKAYSGRALRNELKNNGIKAVIPRKSNE
                     KMASDGRAQLDRDAYRNRNVVERCFGRLKEYRRIATRYDKTARNYLAMVKLGCIRLFY
                     QRLRN"
     gene            complement(3085..>3364)
                     /locus_tag="OO130_004050"
     CDS             complement(3085..>3364)
                     /locus_tag="OO130_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HCR5367246.1"
                     /translation="SLNVYCRNGQVEIDNNIGENALRSVAVGRKNYLFFGSDNGGESA
                     AIIYSLLVTCKLNGVEPEDWLREVIVKLNDWPSNRVHELLPWNFSAVK"
BASE COUNT          991 a          697 c          659 g         1017 t
ORIGIN      
        1 cgttcaccac atgcaatgtg ttgttgcagg ggaacggtct gcaccatccc ctatatcgat
       61 caatataaca aaaatgacaa cgtcagattc agggcacaac ctaaatatat tttaggtcac
      121 ctcccaaatc gtttgcctga taccgctcca tttcttaata aaaaatcaat tgtttttgaa
      181 tctttgcttt tcattgcatt gtcaagcata gtgtccccac tatttgcttt ttgaaatctt
      241 ttgtttacat caaccaatcc attattaata aaatattcta gcactttata gttggcacta
      301 tcttcactaa gtaaatactc aacatcatac agctcacaat acatattgtt gttcattttc
      361 tccatctctg cgatatcttg ttttgtaaaa ttaaatttag atagtaaata aagtgccata
      421 tcagattttg cattgcctag agaataagca agagcttgat gagccatttt tttatcagtc
      481 accttattgt taatttcttc ataattggat gctttaatgg catcctccaa agatatatct
      541 ctgagtctta tctttttgaa gttatctgtg cttagcattc ttggtatatg atactcaagc
      601 tgaaacacaa agtttataat tctatccaga ctaacaggat caatgttttt attataacaa
      661 aactctcgga atctttgatc tttactcttt cttataaaat caataagatg caacccaatt
      721 ccaagcctca tatctttagt attaaatatt ggcacatcag tttttccctg attaccatgc
      781 acatacctgc taacttcatc cacaacctct ttaaagttat ctaagaaaaa tgattggtgc
      841 tcatggtaaa aaacaggtgg aatagcatta tcaaagtggt cggttaatgt catatatcca
      901 tatggagagt catgatcaat gatatacgca ttagcaccaa aatccacagt atgatgtgtt
      961 ttatttagtg ggagtgtttc ttgatgattg gaaatttcaa ccccaaaaaa aacaaaatcg
     1021 tggttagcta tttcttcaat atcctcagag aatgtgtttt tatatgtttg tatattctca
     1081 gacttaattt tatcaaagga cttaatatta agagtattat ttgacaggtt agcgttactt
     1141 tgatgtctga agttaaaatc agaattaata ataatagaga gcaagtgacg ttcatccgaa
     1201 gaaagaggat gagttttttg tattatttca gaaagatagt gtctggacac cttatcgagg
     1261 tgctcaagtg accgaaatga tgaccgtgct ccagatggcg cttcaataga aagatttaat
     1321 gatttgaaac tggtcccttg agagaacatt ttactataac tttgcgctgc tatagacttc
     1381 cttaaaaagc ttttcacagt atcagcacag tgatctaaat gtttttcact tatgccagaa
     1441 gaggatgagt ttgttacgca tgatatgttt ttttgtaatg cttttgtatc tttgttcgga
     1501 tttatttttc catcaacaag atcaatattg ttctgaacat taatatgatt tactgcttca
     1561 ggtattttca taaaacatcc cccattatgt gcgtttttat ctgcagttat ttagaataat
     1621 attatctaac ttaatatttt cttgtatgta ttataacgaa catattttaa tatgttgtac
     1681 gattttcact ctgaaatccc ctgagtttaa tcacacttcc tttactgaaa ggaattattg
     1741 caacagtatt atttgttttt ggatatatat cttgcgcctt gttatgtagt taatgctcag
     1801 aatatgatag ataaaataga gacgcagaac agggcatgct cataacatga tgataacata
     1861 aaaaattagt aacaataaat gaaagaatca tgggcgctca acaaaaagag agctaaatag
     1921 ctgcgcggaa tagtaggctg tgtcccttaa tcatctgagt tatagtaact gtctgtttac
     1981 acaacatatt gaactatggc tcgctacgac cttcccgatg aggcatggac catcatcaag
     2041 cccctgttac ctcctgaacc cgccacacca cgggccggac gcccatgggc tgagcaccgt
     2101 aaaatcatca atggcatgtt ctgggtgtta tgttccggtg caccatggcg cgatttaccc
     2161 gaacgatatg gggcatggaa aactgtttat aaccgcttta accgatggtc aaagtcaggc
     2221 gtgattaata ttattttcaa caggttgctt tcgctacttg atgcaaacgg ctttattgac
     2281 tggtctgcca ccgcgctgga tggcagtaat atccgggcgc tgaaatgtgc cgccggtgct
     2341 caaaaaaaca tcccgatatc gacggagata atgggccggg tcgctctcgc ggcggttttg
     2401 gcaccaaaat ccatctggca acagacggaa gtggcctccc gttaaatatc gtgctaagcc
     2461 ccggacaagc tcatgaaagc cagttcgcgc aacgacttct ggacgggatt ggcgttcagc
     2521 gtcagaacgg cagcatgaaa cgccgtggac atgcggtact ggctgataaa gcgtattcag
     2581 ggcgcgcatt gcgcaatgag ctgaaaaata acggtataaa ggcagtaatc ccccgaaagt
     2641 caaatgagaa aatggcatcg gatggacgtg cacagcttga tcgtgatgcg taccgcaatc
     2701 gtaatgtcgt tgaacggtgc tttgggcggc tgaaagaata tcgccgcatc gccacgcgtt
     2761 acgacaaaac ggcgagaaat tacctggcga tggtgaaact gggctgcatc cgactctttt
     2821 atcaacgctt acgtaattaa gggacacaac ctagatcact tagagggaac tcagcccgga
     2881 ttgtgcgatc tgatcaatcg ccaaatcaaa acaaatcacc aaccggactg atcaatgccg
     2941 atcatagcac caatttcccg tgacgaacga tgcctgatgg agaaagccat ccataaaaca
     3001 catgataaat ctatgttctg cgctttgata attttgacga tgccgttgag tggtaagcgc
     3061 ccagagaagt acgtagcgga aggattattt tacagctgag aagttccagg gcagcagttc
     3121 atgcactcgg ttcgacggcc agtcattgag cttcacgatc acttcgcgca accagtcctc
     3181 cggctccact ccgttcagtt tgcaggtgac cagcagactg tagataatcg ccgcgctttc
     3241 tccaccgttg tcagaaccga agaagagata attttttctt ccaaccgcca ccgatcgcag
     3301 cgcgttttca ccgatgttgt tgtcgatttc cacctggcca ttacgacagt acacgttcag
     3361 gcta
//
LOCUS       DAJTMC010000259         3227 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.263, whole genome shotgun sequence.
ACCESSION   DAJTMC010000259 DAJTMC010000000
VERSION     DAJTMC010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3227
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.263"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(<1..1010)
                     /locus_tag="OO130_004051"
     CDS             complement(<1..1010)
                     /locus_tag="OO130_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HCR5367247.1"
                     /translation="MKIGTVAGTNDSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTDHSEKYSMLSGMRAHILRR
                     DNNYQFIVTINSKNYGCSLDNTDVNWCSVVYLLNNMTVNDNANDVAVTESYKPIWNWK
                     ISQYNVFDIKFETIINPQFADRIYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFAT
                     GYLGIMTEGENFLCRLLTIEDEVLNVLLRENFNEQLDTNINTIISNLNRRDNVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGDQFIIDLLKRIMSINENVHVLAGNHETNC
                     NG"
     gene            complement(1272..2862)
                     /locus_tag="OO130_004052"
                     /pseudo
     CDS             complement(1272..2862)
                     /locus_tag="OO130_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312389.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
BASE COUNT         1034 a          592 c          507 g         1094 t
ORIGIN      
        1 ttaccattgc aattcgtttc atgatttcca gctaatacat gaacattttc attgatactc
       61 attatgcgtt ttaacaaatc gatgataaat tgatcgccac agatattact aaatcgatcg
      121 ccggtttggt cgcccagaaa cgtacatggc gtgactgcgt ctttattaat aactaaataa
      181 ggctgaagac tttctaaaac attatctctt ctgttcaggt tactgattat tgtattaata
      241 ttagtgtcaa gttgctcatt aaagttttcc cttagcaata cgttgagaac ttcatcttct
      301 atagtaagca accggcaaag gaaattttct ccttcagtca tgattcccaa atatcccgta
      361 gcaaaaagcg cgtacaaaac tgcaccaact gaaccatcgg tatccccaaa ataggtcggc
      421 ctggtacttg tgggatcaac aggtgaacaa ttggagaaat agattctgtc agcgaactgg
      481 gggtttataa ttgtttcgaa tttaatatca aatacattgt attgcgatat tttccagttc
      541 cagatgggtt tgtacgactc agtaactgca acatcattag cattatcatt tactgtcata
      601 ttattaagta aataaacaac actgcaccaa ttaacatcag tattatcaag agagcaaccg
      661 taatttttac tattaattgt cacaataaac tgataattat tatccctacg tagaatatga
      721 gcacgcatgc ccgatagcat actatatttt tctgaatgat ccgtttgtat ggtaaatgaa
      781 attttgtcat cttgaataga ttgttggaac cgttctattt gccaatcatc tattttttcc
      841 acaacctcac cgagcattgt cctggcaata cttgatatat catcacctat accatttgca
      901 atatacccaa acagttcttt agtgaagttg gtaacatgct cttgcaccat atcatttgtt
      961 gcgattgtcg ttgtactgtc gttagtgcct gccacagttc caattttcat cttttacctc
     1021 atattattca ccgcgaggaa tacatagtta acattttatc ttatgacata cacctcatgt
     1081 tcgatggaaa aataattata ttttgcacaa atcattcagc cttaaagcat aatatgaaat
     1141 ttcttatttc atagcagaga aagtccctaa agattgtaga ggcgtcatca gaacatgtct
     1201 ctacaatcga ttaagaaaac ctatgaaata ctggcaggat taaaaagata taccacagca
     1261 ctcatggagt tttaatgtgg ttgaaaacaa ctaagccctg ggacaccttt tttatcacca
     1321 cgttcgttag gagctatctt ccaggaattt agccccatgg tataattatc aattttttga
     1381 tgctgatcta cccctaatcc ataatggcca tatttctgtt tagttttttt gagcgtctgt
     1441 ccacatttga agatatcttc aggttttggt ctgaaagcag tagtgttatt aaaataattg
     1501 tcatatacag gtgtggatgc tatagcattg cttctaatta gatggataaa acgatcatcc
     1561 atgtgttgtt tatgtttttt attaaatata tttgctaatt cctcaggatc aattgggtct
     1621 ttcatctgat taaatggaac ctgcaaagca cctagacaat aacattcatt ttcttcattt
     1681 cttattattc cgtgatgact gcttaaaact ttcgtttctg agtcataatt acaaacatcg
     1741 agcgttttaa ttaagttata cgtatcaccg gcagagagtt tatgatttgc tagtcgtgca
     1801 gtatagttac cattgaaatt gatttcataa tttccagcca gaacgacgtc atttttattt
     1861 atacgactat ctttattact ctccatattc cgcatagaat ttagcaaagt aaggatatat
     1921 ttatcaccaa aaatagtact gaatctatcg cctgtatgat ccccaagaaa gatacaaggg
     1981 gtaatcgcat cgccttttaa aaaaacatgt tgatgcaact tattcaagat catgtctctc
     2041 gtatctaata aagtaaagag tttttccaat tttttatttt catatgcgaa tgaagccatg
     2101 tcttcgtgtt ttaataattc acataataaa ctccacccct tttccctgat acctatatgt
     2161 cctgaaacga gcaatgcata aagtacagcg ccaactcttc catctgtatc gccaaaataa
     2221 gccgtgttgg gattttgtgg gttaatcaca ccacactgtt tataaaaatc tatttttgaa
     2281 aacacagaat taacatagct agcaaactta tccttaggaa acgtctcttt agagacatcc
     2341 caacgctagt cagctttaaa attaccgcta agaagagtaa agaaattttc aggtatgata
     2401 gaaaggccat taactttata aattatgtct gatttatctc cggagaaact aataaaataa
     2461 taaccatcct tacactcaat cgtaacggta atgggagacg acaattttat tacatggtta
     2521 ttgtctggta taattgtaaa gataaacgtg tcagtagtat tatcggcgaa actattatat
     2581 tcttgaatat ctatagatgg ctcattattg taaaaccata tttttttcta tctgagaact
     2641 atattcattt accttccagc gaccatgttg caggaaatag atgatatgca ggcctaaatc
     2701 gttatctatt tgcgaatgtt tatccagctt atcaatttca actatctttg aacgatgttt
     2761 tttatcaacc ttgccatggt gttgatgatt gttaacttga tctgaatgct tatcttgatg
     2821 gcttgaagag atgtgcttaa atatattgtg aaatactttc ataaataacc cgcaatattt
     2881 ataacttgtt agtaacaaaa taaacgatac atgtctgaaa gacatattta cgacgataca
     2941 aagtaataat acttatatat ccgatgattt taagcaccga gactatccgg tcttttacta
     3001 aataatggaa aacaatccat ccctggaaga taattgaaaa aaacaacaga ataagaatta
     3061 atcttaggat aaacatttat ttatcatggc cttttgaacg ctgaaaagta gcgctctgta
     3121 aagtaaaccc cgctatagtt atgctatgca tttgtcttta atgatatctc gtcattgtat
     3181 taacaggctg aaattatcat taatgcacat ttcaatatta gcaggga
//
LOCUS       DAJTMC010000260         3224 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.264, whole genome shotgun sequence.
ACCESSION   DAJTMC010000260 DAJTMC010000000
VERSION     DAJTMC010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3224
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.264"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            <1..907
                     /locus_tag="OO130_004053"
     CDS             <1..907
                     /locus_tag="OO130_004053"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HCR5367248.1"
                     /translation="ANIDVAGYSHESLNYADSNPGKIKFTPGGVGRNIAQNLALLGNK
                     AWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLDNTGEMLVAINDM
                     NISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVPVFVDPVSAWKCV
                     KVRDRLNQIHTLKPNRLEAETLSGIALSGREDVAKVAAWFHQHGLNRLVLSMGGDGVY
                     YSDISGENSWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESVRFAQGCSSMALS
                     CEYTNNPDLSIANVISLVENAERLN"
     gene            874..1833
                     /gene="psuG"
                     /locus_tag="OO130_004054"
     CDS             874..1833
                     /gene="psuG"
                     /locus_tag="OO130_004054"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HCR5367249.1"
                     /translation="MVSGERRTSELKISPELLQISPEVQDALKNKKPVVALESTIISH
                     GMPFPQNAQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVS
                     RRDLPFVVAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQEL
                     ANTNVTVVCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSSFDVSIRLDS
                     ASEIARAMAVKWQSGLNGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKES
                     TLFLLARVAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            1927..3177
                     /locus_tag="OO130_004055"
     CDS             1927..3177
                     /locus_tag="OO130_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCR5367250.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
BASE COUNT          771 a          725 c          872 g          856 t
ORIGIN      
        1 ggcgaatatt gatgtcgccg gatattcaca tgaatcatta aattatgcgg attcaaatcc
       61 aggtaaaata aaatttacgc ctggtggagt cgggcgcaat attgcacaaa acctggcgtt
      121 gctggggaac aaagcctggc tactgagcgc cgtaggcagt gacttttatg gtcaatcgct
      181 gctaacgcaa accaatcaat ctggcgttta tgtcgataaa tgcctgattg tgccgggaga
      241 aaatacgtct agttatttat cattactcga taataccggt gaaatgctgg ttgctataaa
      301 tgacatgaat attagcaacg ctattacagc tgaatatctc gcacagcacc gtgaatttat
      361 tcagagggca aaggtcattg tcgcggactg taatatcagt gaagaggcac tggcatggat
      421 tctggataat gccgccaacg tacccgtatt tgtcgatccg gtttccgcat ggaaatgtgt
      481 caaagtgcgc gaccgtctaa atcagatcca cactctgaag ccaaaccgcc ttgaagcgga
      541 aaccctgagt gggattgcgc tgtcagggcg tgaagatgtg gcaaaagttg ctgcctggtt
      601 ccatcaacat ggcctgaacc gactggtatt gagcatgggc ggcgacggcg tttattacag
      661 cgatatcagc ggtgaaaata gctggtctgc gccgatcaaa accaatgtta ttaatgttac
      721 cggagcgggc gatgccatga tggcgggact tgcttcgtgt tgggtagacg gaatgccgtt
      781 tgccgaatct gttcgtttcg cacagggatg ttcgtcaatg gcgctctcct gtgaatacac
      841 caataacccc gatttatcga ttgccaacgt tatatcgtta gtggagaacg cagaacgtct
      901 gaattaaaaa tttcccctga attattacaa atttccccgg aagtgcagga cgctttaaaa
      961 aacaaaaaac cggttgtggc gctggaatcg accattattt ctcacgggat gccgttccca
     1021 caaaatgccc agaccgcaat tgaagtagaa gaaactattc gtaaacaggg cgctgtacct
     1081 gccactatcg ccattattgg cggcgtgatg aaagtgggtt taagcaaaga agaaattgaa
     1141 ttactgggtc gtgaagggca taacgtgacc aaagttagtc gtcgcgattt accttttgtt
     1201 gttgccgccg gaaaaaatgg cgcaaccact gtggcttcaa cgatgattat tgcggcgctt
     1261 gccggaatta aagtatttgc caccggggga attggtggtg tgcatcgcgg ggcggaacat
     1321 accttcgata tttctgccga tttgcaagaa ctggcaaata ctaatgtcac cgttgtttgt
     1381 gccggggcga aatctattct cgatttagga ttaaccactg agtatttaga aaccttcggt
     1441 gtgccgttaa ttggctatca gactaaagcg ctgcctgcgt ttttctgccg taccagctcg
     1501 tttgacgtca gtattcgtct cgacagcgcc agtgaaattg cccgtgcaat ggcggtgaaa
     1561 tggcaaagcg ggctgaacgg tggcctcgtg gtagcgaacc cgatcccgga acagtttgcg
     1621 atgccggagg aatctatcaa tgcagccata gatcaagccg tcgccgaagc cgaagagcag
     1681 ggcgttattg gtaaagaaag tacactgttc ctgctggcgc gcgttgctga gctgaccggc
     1741 ggtgacagcc tgaaatccaa tatccagctg gtattcaaca acgccattct ggcgagtgaa
     1801 attgccaaag aataccagcg tctcgcgggt taatttccga cgggcagaaa gccgtcgccc
     1861 gtactcatca agcataccaa aacctggcgc tgacgccggg tttactggca tgaagggaaa
     1921 atcattatgg atataatgag aagtgttgtg gggatggtgg tgttactggc aatagcattt
     1981 ctgttgtcag tgaataaaaa gagcatcagt ttgcgcacgg ttggagccgc actgctgctg
     2041 caaatcgcta ttggtggcat catgctctac ttcccaccgg gaaagtgggc agtagaacag
     2101 gcggcattag gcgttcataa agtgatgtct tacagtgatg ccggtagcgc cttcattttt
     2161 ggttcgctgg ttgggccgaa aatggatgtc ctgtttgacg gtgcgggttt tatcttcgcc
     2221 tttcgcgtac ttccggcgat tattttcgtt actgcgctca tcagtctgct gtactacatt
     2281 ggcgtgatgg ggctgctgat tcgcatcctt ggcagcattt tccagaaagc tcttaacatc
     2341 agcaaaatcg aatcttttgt cgcagtcaca actattttcc tcgggcaaaa tgagatcccg
     2401 gcgatcgtta aaccgtttat cgatcgcatg aatcgcaacg agttgtttac cgcaatttgt
     2461 agcgggatgg cgtccattgc tggttcgatg atgattggtt atgccggaat gggcgtacca
     2521 attgactacc tgttagcggc atcgctgatg gcgatcccag gtggtatttt gtttgcacgt
     2581 attcttagcc cggccaccga gccttcgcag gtcacatttg aaaatctgtc gttcagcgaa
     2641 acgccgccaa aaagctttat cgaagcggcg gcgagcggtg cgatgaccgg gctgaaaatc
     2701 gccgctggtg tagcgacggt ggtaatggca tttgtcgcaa ttattgcgct gatcaacggc
     2761 attatcggcg gaattggtgg ctggtttggt ttcgccaatg cctctctgga aagtattttt
     2821 ggctatgtgc tggcaccgct ggcgtggatc atgggtgtgg actggagtga tgccaatctt
     2881 gcgggtagcc tgattgggca gaaactggcg attaacgaat tcgtcgctta cctgagtttc
     2941 tccccatacc tgcaaacggg cggcacgctg gaagtgaaaa ccattgcgat tatctccttt
     3001 gcgctttgtg gttttgctaa ctttggttct atcggtgttg tcgttggcgc attttcggct
     3061 atttcgccaa aacgcgcgcc ggaaatcgcc cagcttggtt tacgggcgct ggcagcagca
     3121 acgctttcca acctgatgag tgcgactatt gccgggttct ttattggttt agcttgagtt
     3181 tcattgccgg atgtacgcgc ttattaagcg ttgcatccgg caat
//
LOCUS       DAJTMC010000261         3207 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.265, whole genome shotgun sequence.
ACCESSION   DAJTMC010000261 DAJTMC010000000
VERSION     DAJTMC010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3207
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.265"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(<1..923)
                     /gene="curA"
                     /locus_tag="OO130_004056"
     CDS             complement(<1..923)
                     /gene="curA"
                     /locus_tag="OO130_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HCR5367251.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSHVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVVGGAGGAEKCRHAIEVLGFDVCLDHHAGDFAEQLVKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDK"
     gene            1104..1622
                     /gene="mddA"
                     /locus_tag="OO130_004057"
     CDS             1104..1622
                     /gene="mddA"
                     /locus_tag="OO130_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HCR5367252.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDSGKHVMVAGIESQNQASLHLHHSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            1619..2068
                     /locus_tag="OO130_004058"
     CDS             1619..2068
                     /locus_tag="OO130_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR5367253.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLMLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(2069..2302)
                     /locus_tag="OO130_004059"
     CDS             complement(2069..2302)
                     /locus_tag="OO130_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcY family protein"
                     /protein_id="HCR5367254.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(2388..2561)
                     /gene="ortT"
                     /locus_tag="OO130_004060"
     CDS             complement(2388..2561)
                     /gene="ortT"
                     /locus_tag="OO130_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HCR5367255.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            2756..2851
                     /gene="yncL"
                     /locus_tag="OO130_004061"
     CDS             2756..2851
                     /gene="yncL"
                     /locus_tag="OO130_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HCR5367256.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
BASE COUNT          794 a          812 c          747 g          854 t
ORIGIN      
        1 attttatcct ctttcaccca ttgccccatc tccttctgaa actcatggat gcggtgacca
       61 taatcctgag cgataataaa cccttgcaag cgaatacgtt ttttcagcac cgttgccatc
      121 aacaggggta aacgatccgg accgggtggt agctctgtag cgttatagct gctcactaag
      181 ccacaaacgg gaatgcgcgc agatgtatta agtaatggta gcactgcatc aaatacctta
      241 ccgcccacgt tttcataata gatatcaata ccttttgggc acgctttcac cagttgttcg
      301 gcaaaatcac ccgcgtggtg atcaagacaa acatcgaagc ccagcacctc gatagcatgg
      361 cggcattttt ccgcgccacc ggcccccccc accaccctgc aacctttaag tttgccgatt
      421 tgccccaccg ttgctcccac aggacctgtc gccgcagcta ccaccaacgt ttcgccctct
      481 ttaggctgac cgatatccag cagccccata taagcggtaa agcctggcat ccccagcaca
      541 cccagcgacc acgatggatt ttgcggatga tcgccaagtt tcaccagatc atcaccactg
      601 gatatgtcat agtcctgcca tccactgtag cccagcaccc agtcgccagg ctgataatca
      661 ggatgattcg actccacaac atggctcacc gtgccgccga ccatcacgcc gccaatatca
      721 acaggcggtg aataagatgg ctcatcgctc atacgtccac gcatatacgg gtctagggac
      781 aaataaactg tgcgcagtaa cacctgtcct tcacccggtg tggcgacatc atcttcttca
      841 agacggaaat tctccggaac cggtgcgcca tgtggacgcg aggccagaac ccaacgtcga
      901 ttacgctgct tttgttgccc cataatgctc tcctgtgctt ttggcatgaa tccatcaata
      961 gtagcaggct cacgggattg gttttgtttg cagccgtcac atcccctctg caacacgcaa
     1021 taatttcatc gaggaatccg ttgcttcggg ctgttttcga tagtattgat aacatcatca
     1081 tcattatttt atcgaggcca gacatgtcca tccgttttgc ccgcaaagcc gattgtgctg
     1141 ccattgcgga aatttataac cacgccgtgt tgtatacggc ggctatctgg aatgaccaaa
     1201 cggttgatgc agataaccgc attgcctggt ttgaagcgcg gaccatagca ggttatccgg
     1261 tgctggtaag tgaagaagat ggcgtagtga cgggatatgc ctcgtttggc gactggcgta
     1321 gtttcgatgg ttttcgccat accgtggaac attcggttta tgtccatccc gatcatcagg
     1381 gcaaaggtct ggggcgtaaa ttgttaagcc gattgattga tgaagcgcgg gatagcggga
     1441 agcatgtcat ggtcgccggg atcgaatcgc aaaatcaggc ctcgctgcat ctgcaccatt
     1501 cgctgggatt tgtcgtcacc gcacaaatgc cgcaggtggg cactaaattt gggcgctggc
     1561 tggatctgac atttatgcag ttgcaactcg acgaacgcac tgaaccggac gcgattggat
     1621 gaatcagtcg ctcacccttg cttttttgat tgccgccgga attggtctgg tagtccagaa
     1681 cacgctgatg gtgcgcatca cccagacctc ctctaccatt ctcatcgcca tgttgctgaa
     1741 ctcactggtg gggattgtgc tctttgtttc tattttatgg tttaaacaag gcatggcggg
     1801 gtttggcgaa ctggtgtcca gcgtgcgctg gtggacactt atacccggct tgctgggatc
     1861 gttttttgtc ttcgccagta tcagcggata tcagaatgtg ggagcggcga caactattgc
     1921 agtactggtc gccagccagc taattggcgg tctgatgctg gatatcttcc gcagtcatgg
     1981 cgtaccacta cgggcgttgt tcggcccaat ttgtggcgca attttacttg tggttggcgc
     2041 ctggttggta gccagacgct cattttaatc agaggatggt gccgcctttg gttaactgtt
     2101 cgtggcgcgc ttccatatct tctttatgct tgcgaccgtg atgagcaata gcggtgcgca
     2161 aacgctgctg ctgggtgtag cgatcttccc gacttaactc cgcgtcatcg ctcagggcga
     2221 ttaataattc gttcatatgg gcaatgacgc tctcttcaat cgcggcatcc acgcgcgcga
     2281 tgacttcatc cagatgcgac atactttctc cttaaccact tgtgaaattt atcgattcat
     2341 ccaaatgata taagtctgcg tggtgccgtc aagccagtga gtaagattta aaacagtgaa
     2401 aacaacagcg ccaccgggaa actcatcggc catgttgccc ccaccagaaa ggcgcttaag
     2461 aaacggatgc gtttcttatc gtgagaaaga aaccaggtga taagaaatgc gattgctgcc
     2521 ataaccgcat aaaaaacaag catatgttga tagagagaca tgtacgggcg tccagccaga
     2581 tgaataaaac gcgcgcaaat atgcgtcatt ttttgacgtg catcaagttt tttcaccagt
     2641 ttgagaaaaa atcatcaaac agcaacttgc ccttatccat cttcagaatg agtattattg
     2701 cggattttct taacccgtac tatacacata ggggcaatga taaaaggtgg caaaaatgaa
     2761 tgtttccagt agaactgtag tactgataaa tgtctttgct gctgttggtt tgtttactct
     2821 tatctctatg agatttggct ggtttatttg atgtagtgat tttgcacgca gcacgtactg
     2881 tcagagaaat caactctctg acagtacgca agatcttctc atactctcca ggtttacaac
     2941 attatcctta atatttactt ctttttgctg gcatctacat tgaaatgagt agaaaacatc
     3001 cccctactgt cgtctataca aacagcttac tgattcaaac ggaatcttgt tttcacattt
     3061 ttctatcatg aatactttat acgcgcggga gtctataaat gctgaggtag cctgagttta
     3121 acggacactc cttcctgaaa tagaatggca tcagaaggag ctaataatga gcagaaaaaa
     3181 ccaacgttac tctaaagagt tcaaagc
//
LOCUS       DAJTMC010000262         3195 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.266, whole genome shotgun sequence.
ACCESSION   DAJTMC010000262 DAJTMC010000000
VERSION     DAJTMC010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3195
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.266"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            199..939
                     /locus_tag="OO130_004062"
     CDS             199..939
                     /locus_tag="OO130_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HCR5367257.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(910..1677)
                     /locus_tag="OO130_004063"
     CDS             complement(910..1677)
                     /locus_tag="OO130_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HCR5367258.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(1883..2461)
                     /gene="lpcA"
                     /locus_tag="OO130_004064"
     CDS             complement(1883..2461)
                     /gene="lpcA"
                     /locus_tag="OO130_004064"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HCR5367259.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            2701..>3195
                     /gene="fadE"
                     /locus_tag="OO130_004065"
     CDS             2701..>3195
                     /gene="fadE"
                     /locus_tag="OO130_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="functions in fatty acid oxidation; converts
                     acyl-CoA and FAD to FADH2 and delta2-enoyl-CoA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HCR5367260.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFF"
BASE COUNT          765 a          810 c          819 g          801 t
ORIGIN      
        1 atcttaggaa ataaatgata catgcgcttt tcggactatt gaaaactgcc cctgtgctaa
       61 atttgtaaac cctgcgccga caggcttgcg taaaactctt ttggtgataa tgtgagcctt
      121 ccaaatccag actattccga tttgggatcc attttgccgt cctggcgtgt gtaaccgttt
      181 tatcaaggaa taagcagtat gcgtaaaatc gcattaattc ttgcgatgtt gttgatcccg
      241 tgcgtttcgt ttgccggttt gctgggcagc agtagctcga ccacgccagt gagcaaagag
      301 tataagcagc agttgatggg atcccctgtc tacatccaga tcttcaagga agaacgtacg
      361 ctcgatctct acgtcaaaat gggcgagcaa tatcagctgc tcgacagtta taaaatctgt
      421 aaatattccg gcggcttagg gccaaaacag cgtcagggcg atttcaaaag cccggaaggg
      481 ttttatagcg tccagcgtaa tcagttaaaa ccagacagcc gttactacaa agcgattaat
      541 attggtttcc ccaatgccta tgaccgtgcg catggttatg aagggaaata cctgatgatc
      601 cacggcgatt gtgtttccat cggctgctac gcaatgacca atcagggtat tgatgagatc
      661 ttccagttcg ttactggtgc gctggtgttt ggtcagccga gcgtgcaagt gagtatttac
      721 ccgttccgca tgaccgacgc caatatgaag cgccataaat attccaactt taaggacttc
      781 tgggagcaac tgaagccggg ttacgactac tttgagcaaa cccgtaagcc accgaccgtt
      841 tctgtggtca atggtcgtta cgtggtcagc aagccgttaa gccacgaagt tgtgcagcca
      901 caactggcat caaactacac gctccccgag gcaaaataag cgccattcgc ctggcataat
      961 cttttgccag gtttcattgc ccgtcagcgg ctgtgtcgca atcaccgtga ccacatcatt
     1021 cggtgtggtc tgcgagctga agtcgatttc tacatcctga tccagcagcg ttgccacgcc
     1081 aaacggtgcg cggcgggtga tccagtgcag attcgtcgag caatacgcca ttacatagcg
     1141 cccatccgaa agcagcatgt tgaaaacgcc cttctgccgc agttcgtccg ccagtgaagc
     1201 gatatattta aacactgctg ccatgttgcc cggcgtgcgc gggtaacgct gcgttaattt
     1261 atgcagcagc cagcagaagg ctttttcgct gtcggtttcg cctaccgggc ggaagttgcc
     1321 ggtttccagt gatttgtagc ccgtcagttg tccgttatgg gcataagtcc agttacgccc
     1381 ccataactcg cgggtaaatg ggtgagtatt ttccaacgcc acctcgcccc gattagcctg
     1441 gcgaatatga gccaccaccg aacaggattt tatcgggtag tcctggacaa gtttggcgat
     1501 gggggaatta aagctgggtt gtggatcttt aaatgtgcga cagcctttac cttcgtaaaa
     1561 ggtaatgccc cagccatctt tatgtggccc ggttccacca ccacgctgta caagtccggt
     1621 gaaactaaag cagatatcgg tagggacgtt ggcgctcatc ccgagcaatt cgcacatggg
     1681 taacctccac aacagcggga gcgcatggcc cccggcgaag attgatttaa gaattttcag
     1741 gtcggatgcg gcgtaaacga attattcggc ctacggttcg cgtacagatt tgtaggcatg
     1801 ataagacgcg tcagcatcgc atcaggcatc agcgcacaag tgccggatgc ggcgtaaacg
     1861 ccttatccgg cctacgccag acttacttaa ccatctcttt ttcaattaac tggatcagga
     1921 tatggatcac tttaatgtga atctcctgaa tgcggtcggc ataaccaaag tgcggtacgc
     1981 gaatttcgat atccgccgtg ccagccattt tgccgccgtc tttaccggtc agggtgatca
     2041 ctttcatccc cttctcacgc gccgctgcga tcgctttgat cacgtttgca gagttaccgg
     2101 aggtggagat ccccagcagt acatcgcctt cgcgacctac cgcttcaacg tagcgggaga
     2161 aaatatcatt gaaaccgaaa tcattaccga cgcaggaaat atgactaacg tcagaaatag
     2221 caatcgccgg gtagcccgga cggttttcac ggtagcgacc ggtcaactct tcggcaaagt
     2281 gcatagcgtc gcaatgggaa ccgccgttgc cgcaggaaag cactttgcca ccggctttaa
     2341 agctgtctgc taacaggacc gccgcgcgct gaatggcgtg aatattggcg tcatctttta
     2401 aaaagttagc cagcgtttcc gccgcttcgt tcagttcgtt acgaataaga tcttggtaca
     2461 tgaggatatc cttcagcata aatgtaatag acaaaatgca gtgtaccgga taccgccaaa
     2521 agcgagaagt acgggcaggt gctatgacca ggactttttg acctgaagtg cggataaaaa
     2581 cagcaacaat gtgagctttg ttgtaattat attgtaaata cattgctaaa tgtatttaca
     2641 tccactacaa ccatatcatc acaagtggtc agacctccta caagtaaggg gcttttcgtt
     2701 atgatgattt tgagtattct cgctacggtt gtcctgctcg gcgcgttgtt ctatcaccgc
     2761 gtgagcttat ttatcagcag tctgattttg ctcgcctgga cagccgccct cggcgttgct
     2821 ggtctgtggt cggcgtgggt actggtgcct ctggccatta tcctcgtgcc atttaacttt
     2881 gcgcctatgc gtaagtcgat gatttccgcg ccggtatttc gaggtttccg taaggtgatg
     2941 ccgccgatgt cgcgcactga gaaagaagcg attgatgcgg gcaccacctg gtgggagggc
     3001 gacttgttcc agggcaagcc ggactggaaa aagctgcata actatccgca gccgcgcctg
     3061 accgccgaag agcaagcgtt tctcgacggc ccggtagaag aagcctgccg gatggcgaat
     3121 gatttccaga ttactcatga gctggcggat ctgccgccgg agttgtgggc gtaccttaaa
     3181 gagcatcgct tcttc
//
LOCUS       DAJTMC010000263         3175 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.267, whole genome shotgun sequence.
ACCESSION   DAJTMC010000263 DAJTMC010000000
VERSION     DAJTMC010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3175
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.267"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            347..506
                     /locus_tag="OO130_004066"
                     /pseudo
     CDS             347..506
                     /locus_tag="OO130_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066419.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(724..2814)
                     /gene="iha"
                     /locus_tag="OO130_004067"
     CDS             complement(724..2814)
                     /gene="iha"
                     /locus_tag="OO130_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional siderophore receptor/adhesin Iha"
                     /protein_id="HCR5367261.1"
                     /translation="MRITTLASVVIPCLGFSASSIAAAEDVMIVSASGYEKKLTNAAA
                     SVSVISQEELQSSQYHDLAEALRSVEGVDVESGTGKTGGLEISIRGMPASYTLILIDG
                     VRQGGSSDVTPNGFSAMNTGFMPPLAAIERIEVIRGPMSTLYGSDAMGGVVNIITRKN
                     ADKWLSSVNAGLNLQESNKWGNSSQFNFWSSGPLVDDSVSLQVRGSTQQRQGSSVTSL
                     SDTAATRIPYPTESQNYNLGARLDWKASEQDVLWFDMDTTRQRYDNRDGQLGSLTGGY
                     DRTLRYERNKISAGYDHTFTFGTWKSYLNWNETENKGRELVRSVLKRDKWGLAGQPRE
                     LKESNLILNSLLLTPLGESHLVTVGGEFQSSSMKDGVVLASTGETFRQKSWSVFAEDE
                     WHLTDALALTAGSRYEHHEQFGGHFSPRAYLVWDVADAWTLKGGVTTGYKAPRMGQLH
                     KGISGVSGQGKTNLLGNPDLKPEESVSYEAGVYYDNPAGLNANVTGFMTDFSNKIVSY
                     SINDNTNSYVNSGKARLHGVEFAGTLPLWSEDVTLSLNYTWTRSEQRDGDNKGAPLSY
                     TPEHMVNAKLNWQITEEVASWLGARYRGKTPRFTQNYSSLSAVQKKVYDEKGEYLKAW
                     TVVDAGLSWKMTDALTLNAAVNNLLNKDYSDVSLYSAGKSTLYAGDYFQTGSSTTGYV
                     IPERNYWMSLNYQF"
BASE COUNT          795 a          838 c          732 g          810 t
ORIGIN      
        1 ctgacctctc agtaaatatc aatacggttc tggcgagccg cttacgtgca gcagtcttat
       61 ccggcagttt aaaggggagg acggagacca atgaggcact ggagcagatt cagtgcagag
      121 gtgacctgct ttatcaaact taattgtgac aaaatggcga ttgagaaggt gcaattgact
      181 tatccggatt cggtcgtggc gaggtggtgc ccctgcattc tgctaaccgg gttatgagcc
      241 aggagtcagc gatagacaaa gggacttatc tgaccgggcc cccggaaaaa tggcgggaga
      301 gaatgatacc ttcctgctga cgagaccgga ctttgagatg acgagcgata gtgcggcagt
      361 gatgtacagc ctgatcggta gctgcaaaac ttaacggaat cgagccggaa acgtggctac
      421 gccacgtgat cagtgtaatc aacacctggc ctgccaaccg catgaaagag ttgttgccct
      481 ggaacgtcac tctctctgta aactaatttt taccccacgt ccttcacggt gcgcttacac
      541 tctggacaag tatgtaaatg ataagttaaa aataatccgt gcatttcctg tgacgatatt
      601 agttgttggt aacgaaatct cattagcgga tcggaaatgc acttaaccta atacgttgcc
      661 actgttccgc caggagttgc agtggcaacg tattctaccg tcagtgatag cgttttgtta
      721 ttatcagaac tgatagttca gcgacatcca gtaatttcgc tcaggtatca catatcctgt
      781 tgttgatgat cccgtctgga agtaatcacc ggcatacagc gtactcttac cggcactgta
      841 caggctcacg tcactgtaat ccttgttgag caggttattc accgcagcat tcagcgtcag
      901 ggcatccgtc atcttccagg acagacctgc atccaccacc gtccaggctt tcaggtattc
      961 tcctttctca tcatacactt tcttctgtac agcgctcagt gacgaataat tctgggtgaa
     1021 acgtggtgtt ttcccgcgat aacgggcacc cagccatgat gccacctctt cggtgatctg
     1081 ccagttcagt ttcgcattca ccatgtgttc aggggtataa ctcagcggcg cacctttgtt
     1141 atcaccatca cgttgttcac ttcgggtcca ggtgtaattc agtgacagcg tgacatcctc
     1201 tgaccacagc ggcaatgtgc cggcaaattc cacaccgtgc aaccgggcct ttccgctgtt
     1261 tacatagcta ttggtgttat catttatgga ataagagaca atcttgttgg agaagtcagt
     1321 cataaaacct gtgacattgg cattcagacc ggcggggtta tcgtaataca ccccagcctc
     1381 ataactgacg ctctcttccg gcttcaggtc ggggttacca agtagatttg tttttccctg
     1441 cccggacaca ccactaatcc ctttatgtag ctgccccatt ctgggtgcct tatatcccgt
     1501 ggtcacaccg cctttcagcg tccaggcatc tgccacatcc cagaccagat atgcacgcgg
     1561 actgaagtgt cccccgaatt gctcatgatg ttcatagcgg ctgcccgcag tcagcgcaag
     1621 tgcatccgtg agatgccact catcctcagc aaataccgac cagcttttct gccggaaagt
     1681 ttcacctgtg ctggcaagga caactccgtc tttcatggac gagctctgaa actcgccccc
     1741 caccgtaacc agatgagatt ctcccagagg ggtaagcagt aatgaattca ggataaggtt
     1801 cgattcctta agctcccgcg gctgaccggc aagcccccat ttgtcgcgct tcagtacact
     1861 gcgtacgagc tcacgacctt tattttctgt ctcgttccag ttcagatacg atttccatgt
     1921 tccgaaggtg aaagtatgat catagccagc tgaaattttg ttccgctcat agcgcagggt
     1981 ccggtcatat ccccccgtca gactccccag ttgcccatcc cggttatcat aacgctgccg
     2041 ggtggtatcc atatcaaacc agagcacatc ctgctccgac gccttccagt caagacgtgc
     2101 accaagatta taattctgtg actccgtggg ataaggaata cgcgtggctg ctgtatcgct
     2161 cagtgatgtg accgatgagc cctgacgctg ttgtgtgcta ccgcgtacct gcaggctgac
     2221 agaatcatcc acaagggggc cactgctcca gaaattaaac tggctgctgt taccccattt
     2281 gttgctttcc tgcagattca gccctgcatt gacggaagag agccatttgt ctgcattctt
     2341 tctggtaatg atattcacca caccgcccat cgcatcagag ccatacagtg tggacatcgg
     2401 ccccctgata acctcaatac gctcaatggc ggccagaggg ggcatgaacc cggtattcat
     2461 ggcagaaaaa ccgttgggag tcacgtcact gcttccgccc tgacgaacac catcaatcag
     2521 tatcagcgtg taactggctg gcattcctcg gatgctgatt tccagcccac cggttttacc
     2581 cgtaccactt tcaacatcca caccctctac tgatctcaga gcctccgcca gatcgtggta
     2641 ctggctggac tgcaattcct cctggctaat cacagaaaca ctggcggctg cgttagtcag
     2701 ctttttctca tagccggatg ccgagacaat catcacatcc tctgcagcag ctatgctgct
     2761 ggctgaaaat ccgagacagg gaatgactac ggaagccaga gtggttattc gcatattact
     2821 aatctccgat catgttaacc ggcaagacaa tacacaaata caaacaataa tgagaataat
     2881 ttatatttgc cttttacaaa aaataaagat tattaacaaa actaaatata aataacaact
     2941 gcctcggcat gccagttaac agctatccgt atggcgtgtt cggttttgcg aataaaaaat
     3001 ggttactgta gaagcggatt tccaccacat gtcaaccatg aatggtcagt ggtgcgagac
     3061 gactgggatg atttggtcga tgacttcttc cggtgaatac tgatagaaac attgaaatat
     3121 tgtaagagaa agcgttcaaa attctgtggc acgtgaaaaa ttctacagcg tcatc
//
LOCUS       DAJTMC010000264         3144 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.268, whole genome shotgun sequence.
ACCESSION   DAJTMC010000264 DAJTMC010000000
VERSION     DAJTMC010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3144
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.268"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            <1..141
                     /locus_tag="OO130_004068"
     CDS             <1..141
                     /locus_tag="OO130_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="activator of the allDC-ylbA operon involved in
                     allantoin utilization; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HCR5367262.1"
                     /translation="MRPPSPLSLAWRKFGSGKAVEDIVTLFTQRRPEISGFLEIFGNP
                     RS"
     gene            210..1304
                     /gene="mnmH"
                     /locus_tag="OO130_004069"
     CDS             210..1304
                     /gene="mnmH"
                     /locus_tag="OO130_004069"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HCR5367263.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRIDAWRAACLQNPQGILCCARGGQ
                     RSHIVQSWLHAAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKAR"
     gene            1420..1785
                     /locus_tag="OO130_004070"
                     /pseudo
     CDS             1420..1785
                     /locus_tag="OO130_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014543172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(2347..2796)
                     /locus_tag="OO130_004071"
     CDS             complement(2347..2796)
                     /locus_tag="OO130_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001348602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5367264.1"
                     /translation="MLAISSNLSKMIIFIFAIIIIVVLCVITYLYLYKDESLVSKHYI
                     NYMAIPENDGVFTWLPDFFSHVAVDISIYTNVEDDYFFSYFSLTNDDGGRFKKTLTVR
                     AREQVAKIVSKNDPDTKKVWCKYGKIPGQGDGVNLFLLVKLMLRIIL"
     gene            complement(2777..3144)
                     /locus_tag="OO130_004072"
                     /pseudo
     CDS             complement(2777..3144)
                     /locus_tag="OO130_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308587.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
BASE COUNT          841 a          727 c          712 g          864 t
ORIGIN      
        1 atgcgccctc cttcgccatt gagtctggca tggcgcaaat ttggcagcgg caaagcggta
       61 gaagatattg tgaccttgtt tacccagcgc aggccggaaa tcagcggatt tttagaaatt
      121 ttcggcaacc cacgcagtta aaaatcaagt ttccttgcgc gttggcgcgt aaaatagcgc
      181 gcaagctgat ttgaggttca cttttaacta tgcaagagag acacacggaa caggactatc
      241 gtgccctgct gattgctgat acgcccatta ttgatgttcg cgcccctatc gagtttgagc
      301 aaggcgcaat gcccgccgct atcaatctgc cgttaatgaa taacgatgaa cgcgccgccg
      361 ttggcacctg ctataaacag caagggtcag acgcggcgct ggcgctgggg cataaactgg
      421 tggcgggtga aattcgtcag cagcgcatag acgcctggcg ggcagcgtgc ctgcaaaatc
      481 cgcaaggtat tctctgctgt gcccgtggcg gtcaacgctc acatattgtg caaagctggt
      541 tgcatgcagc ggggattgat tatccgctgg tggaaggcgg ttataaggca ctgcgccaga
      601 ccgcgattca ggcgactatt gaactggcac aaaaaccgat agtgctgatt ggcggttgta
      661 ccggcagcgg taaaacgctg ttagtgcagc aacagccgaa cggtgtcgat ctggaagggc
      721 tggcgcgtca tcgcggttcg gcgtttggtc gcacgttaca accacaactt agccaggcga
      781 gttttgaaaa tctgctggct gccgaaatgc taaaaaccga cgcccgtcag gatttgcgcc
      841 tgtgggtgct ggaagatgaa agccggatga tcggttcgaa tcacctgccg gaatgtctgc
      901 gcgagcgaat gactcaggcg gcgattgcgg tggtagaaga tccgtttgag atccgtcttg
      961 aacgcctgaa cgaagagtat ttcttgcgta tgcatcatga ttttacccac gcgtacggcg
     1021 acgaacaggg ctggcaggaa tattgcgaat acctgcatca cggactttcg gctattaagc
     1081 gtcggctggg gttacagcgc tataacgaac tcgctgccca gctggatacg gcgctgacaa
     1141 cgcaactcac caccggcagc accgacggtc atctggcctg gctggtgccg ttacttaaag
     1201 aatattacga cccaatgtat cgctatcagc ttgagaaaaa agcggaaaaa gttgtctttc
     1261 gcggtgagtg ggcggaagtg gcggaatggg ttaaggcgcg gtaatgaggg acgtatttgt
     1321 attagtcgcg acttgatctg acacatggcc ttgaaaggtt gagagttacc ggttttgata
     1381 tgagtgtcga atccttatcc aaaacaagag gtaactctca tgcttcatac tgccaatccc
     1441 gtcatcaaac acaaagccgg tttgctcaat ctggctgaag aactcagcaa cgtatcaaaa
     1501 gcctgtaaaa tcatgggcgt ttcgcgtgat acgttttatc gttaccgcga actggtcgct
     1561 gaaggcggtg tggatgcgca gattaatcgt agtcgccgcg ctcctaacct taagaaccgt
     1621 accgatgagg caactgaaca ggctgttgtt gattacgccg tcgctttccc gacacatggt
     1681 cagcaccggg ccagcaacga gctgcgtaaa caggggggtt ttatctccga cagtggtgtc
     1741 cgttctgtct ggttactcca taaccttgag aatctcaaaa gacgatactg aacaaatttt
     1801 atcaggtgac gttccgtaaa aagttgtatg gagttctcga tgcagtcggc gcgactatca
     1861 gtacccccgc tgaaccctgg actatcgggc cgccgtactg gggcatgtca ccctggcgtg
     1921 ccgctggttt tccgctaatg gtttatttcc tctttctttc ccggcaagaa tttattttaa
     1981 tccggaagga ttatttattt tcagataggg actcacatta aacacgtaac attttaatta
     2041 atgcatggct gaatatgtat tacacaatgt cagatatcgt aaaagaccag tgcaattcta
     2101 tgtaaactct ctgactgttc attttatttg ttgtttcagg gtcggaactc accctcaata
     2161 aacaactgaa tacgagtaga tattcatcgt ctgagctata tggctttaca caatagccga
     2221 catgtctttt ctcagtcagg aattttactt ttcctcacta cgaagttgac gacttcgata
     2281 tgggatagac tcttaattca agcaattact ctgcacaagc atcaggcaat ccagctccaa
     2341 tatttgttat aaaataatgc gtaacattaa tttcaccaac aaaaaaaggt ttacaccatc
     2401 cccttgccct ggtatcttac catatttaca ccacactttt tttgtatctg gatcattctt
     2461 tgatacgatt ttcgccactt gttccctggc cctcactgtc aatgttttct taaacctacc
     2521 cccatcatca tttgttaggg aaaaataaga aaaaaaataa tcatcttcta catttgtgta
     2581 tattgatata tccaccgcta cgtgcgaaaa aaaatccggg agccatgtaa aaactccatc
     2641 attttctggt attgccatat agtttatgta atgtttactt acaagagatt catctttgta
     2701 taaataaaga taagtaatta cgcataaaac aacaatgatt ataatagcaa aaataaatat
     2761 tatcatcttt gatagattac ttgagatagc cagcatcttg taaagccttt atcgtttttt
     2821 tatgctctgg attaatataa tcactacatc tatctgagca atctgttgtt gatggacatg
     2881 tcaacccatg gtcatttaca gccaagtctt ttttattatc ttcaatcatt tcagaatggg
     2941 atagctttac ttttctgccg tacataccga acagatttaa tccgtaatct ctaatctctt
     3001 tttcataacc cagccctttc gccgatcggc taacaccagc gtcatttaat ttagacactc
     3061 gacaaaatgc catacaatgg aaaaattgat cagtcccacc taaatttaat ctcttcataa
     3121 gtatcagata atatatccca tgct
//
LOCUS       DAJTMC010000265         3119 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.269, whole genome shotgun sequence.
ACCESSION   DAJTMC010000265 DAJTMC010000000
VERSION     DAJTMC010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3119
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.269"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(13..780)
                     /locus_tag="OO130_004073"
     CDS             complement(13..780)
                     /locus_tag="OO130_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5367265.1"
                     /translation="MQPCPAMQALIEQIYHTFRRYRVPQKFVVCCEYCLSQQEQKALR
                     STSLRAIPYSLINAWNSSPGPDPQNSDEVRYFLPRLLEFVAQGHFDNIHVVFSLRRIN
                     LANKENWRADERAILQRFACQYMTDWVSGDEAVELQYMLEMFFRADIALAPLLDAINS
                     VPGFWSTVSLACLLNRYCEDYIRDNQDDIDNVITTQINAWARNNHPLLKERARQAIEN
                     PLKQPEPMTEYQVWEDDWIIDECLCAMYDASSESLGK"
     gene            complement(783..1493)
                     /locus_tag="OO130_004074"
     CDS             complement(783..1493)
                     /locus_tag="OO130_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5367266.1"
                     /translation="MELVNTLFASLAGTDPFTGVDITIANCKSAYWDEGIVQQLINQA
                     LDEGEKFVGADGLEGLLRYDVTLNIGLTSSKVWPGFSLDTATISRLCACGADFGFDPY
                     ISDVPDVQCDLNTTNDVTVQFTAMLNPDERVIIAKRPLKKCDSWIEDVYIFQVFKDAW
                     KFHNNNSLRGFRDKQAELKLYTRHYSVENCAEESCRDCNSCIRPSFSLSRSALIRLNA
                     ANARFVYQPFTRDQRARG"
     gene            complement(1509..3014)
                     /locus_tag="OO130_004075"
                     /pseudo
     CDS             complement(1509..3014)
                     /locus_tag="OO130_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001472437.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
BASE COUNT          850 a          761 c          688 g          820 t
ORIGIN      
        1 atctaatcat ttttattttc ctagtgattc ggatgatgcg tcatacatcg cgcataaaca
       61 ctcatcaatt atccagtcat cttcccagac ctgatattct gtcattggtt cgggctgttt
      121 tagcgggttc tcaatcgctt gccgcgctct ttctttcagt aaaggatgat tatttctagc
      181 ccaggcattg atttgggtgg taatgacgtt atcgatatcg tcctggttat ctctgatata
      241 atcctcacaa tatctgttca ataaacaggc aagagaaacg gtcgaccaaa agccgggaac
      301 tgaattaatg gcatccagca atggcgcaag agcgatatcg gcacggaaaa acatttccag
      361 catatactgt aattcgacag cttcatcgcc ggatacccaa tcagtcatat actggcaggc
      421 aaatcgctgt aaaattgccc gttcatccgc acgccagttt tctttgttgg caaggttgat
      481 acggcgcaga gaaaaaacta cgtgaatatt gtcaaaatgc ccctgtgcaa caaattccag
      541 taaacgcggc agaaaatagc ggacctcatc gctattttgc ggatcaggcc ctgggctact
      601 attccaggca ttgattaagg aatacgggat cgctcgtaac gatgtactcc gtaatgcctt
      661 ttgttcctgc tgactcaggc aatattcaca acaaaccaca aatttctgcg gcacccgata
      721 acgacgaaag gtgtgataaa tctgctcaat taatgcctgc atggcggggc agggttgcat
      781 agttaccccc tcgcccgctg atcgcgtgta aacggttgat agacaaaacg agcattcgcc
      841 gcgttcaggc gaataagggc gctacgcgaa agggaaaagc tggggcgaat gcaactattg
      901 cagtctcgac agctttcctc tgcgcaattt tccactgagt aatgacgcgt gtagagtttc
      961 agttcggcct gcttatcgcg aaatccgcgt aaactgttat tgttatgaaa tttccaggca
     1021 tctttaaaga cctgaaagat atagacatcc tcaatccagg agtcacattt tttaagcggg
     1081 cgctttgcta ttatcacccg ctcatccgga tttagcatcg ccgtaaattg tacggtgacg
     1141 tcatttgtcg tgttcaggtc acattgaaca tctggtacat cactgatata ggggtcaaat
     1201 ccaaaatcag caccacaggc acaaagacgg ctaatggtgg cggtatcgag ggaaaatccc
     1261 ggccatactt tgctggaggt taagccaata tttaatgtga catcatatcg aagcaatccc
     1321 tcaagaccgt cagcaccgac gaacttttcc ccttcatcca gggcctggtt aataagttgc
     1381 tgaacaatgc cttcatccca gtatgccgat ttgcagttgg caatcgtaat atccacgcct
     1441 gtgaagggat cagtaccggc aaggcttgca aataatgtat taactaattc catttttatt
     1501 atccagcttt aaggtagcgt cttatacatc gggacataac ctacatcttc caccaaactc
     1561 tgtccctgcg gcgttaaaaa ccactccaac aatttttgtg tctctgacgt cgtattttcc
     1621 cgcgttacca taaaggcatc gatgatatag ggatatttgc cgttgcgaat gttttcggca
     1681 gtcggtgcaa taccgttaat cgctagcagt tttatatttt tatcggcgtt catttgtgtt
     1741 gcgtaataac ggaaggtgta acctattgcg ttgtttgtat tacggtattc ggcaacgact
     1801 ttaatcattc cctccatcat acttgccact tctgtttcct gcggcgagat catgcggaca
     1861 tttttcatga cctgggattg catcaccgtc tggctaccag agtcttccgg gcgttgctag
     1921 gtctggatct cctgatcgtt accgccaaca gtacgccagt tggtaattgc accactgaag
     1981 atgtcacgca cctgttgttc agtcagggaa ttaaccggat tatccgcatt gacgatgaaa
     2041 acaaacgctt cacgggcaaa aggcgtgtac agcaaagtaa tgcccgattc ctcagcgcgt
     2101 tttttctgcc caccggaagg ttgcgccaca aaaataatat cagcatcacc tttaacaatc
     2161 ctgttatacg catcgggggt acgagagctc tccagatatt ccctggtgtg aaaatcttcc
     2221 ggtataacgc tcaaagcata aaatgcagag gcataaattg ggtacgccgc cgtagcacca
     2281 tcgagtcgcg gccagttttg cgtaaactga atttgcggtt taccacgcag tggtgtcagt
     2341 tgattattga gtttatcggg tcgccaggcc caggtatcaa tattttcgcg cacaccgacg
     2401 ccgggattga gctgtttata taaataaccc tgataaccag cgacaaaacc aagcatgaga
     2461 aggataaaca ggagggaatt acggcaacgt agcccggatg taccggttaa aggatgtctg
     2521 gttggccggc cgtaccccag cgaaaaactg atatgcgcgc caacgggtat aatgactatc
     2581 caccagtact cacctccgac tactgagact aacaaaacgc ccagccaggg gagcataaga
     2641 tcagccaacc aacccgatcc catctggtga aattgataat ctgccagatg cagcgcgatt
     2701 atccagccaa ccaggattaa taaaccgggt actaaaaaaa gacattgtcg ccagacggct
     2761 atttctcttt tttctggtgc actatcgcct gcccatactc gccccgagaa aaatagcatt
     2821 aatgcgccca gcgccagcat aaaaatgttg agcagaaggt aacttgttgg cgtgataagg
     2881 atattacaaa cttcaataat accggcaagt ccgacaccca gcatagcaac aaccgccgcc
     2941 aaaaattgcg tcagtatggg gatgccgaaa aaagtcatta ccagcgagga caaaatccat
     3001 tttctgtttt gcattattct ttccattctt tttgaatggt gaaattatac tccccgagtc
     3061 cccttgcccc ttctggacac ttttccgaaa tgatggcgga aaaaaacggg accctttgg
//
LOCUS       DAJTMC010000266         3103 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.270, whole genome shotgun sequence.
ACCESSION   DAJTMC010000266 DAJTMC010000000
VERSION     DAJTMC010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3103
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.270"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(<1..329)
                     /locus_tag="OO130_004076"
     CDS             complement(<1..329)
                     /locus_tag="OO130_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5367267.1"
                     /translation="MSALYERSQLTQVMISSAPATAETMDKAEYLRLDCTIKEVQFTA
                     GQKQDIDVTTLCSTEQENINGLGASSEISMSGNFYLNQAQNALRDAYDNDALYAFKVL
                     FPSGKGF"
     gene            complement(395..739)
                     /locus_tag="OO130_004077"
     CDS             complement(395..739)
                     /locus_tag="OO130_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HCR5367268.1"
                     /translation="MTEADLYPHLAHLAGGQVSPYVAPLLDGRPSVALPWVVFSLISS
                     VSADVMGGQAESSVSVQIDVYAGTVTQARQIRQDAREAIMLLAPGSVSEMQDYIPENR
                     CYRATLEFQVTV"
     gene            complement(736..1182)
                     /locus_tag="OO130_004078"
     CDS             complement(736..1182)
                     /locus_tag="OO130_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HCR5367269.1"
                     /translation="MIEILLDFSGLEDISRDLQLLSGAENNRVLREATRAGANVLKEE
                     VVSRTPVRRGKLRRNVVILSRRSRDGGMESGVHIRGVNPDTGNSDNTMKADNPRNAFY
                     WRFVEMGTVNMPPHPFVRPAFDVRSEQAAQVAIARMNRAIDEVLRR"
     gene            complement(1179..1499)
                     /locus_tag="OO130_004079"
     CDS             complement(1179..1499)
                     /locus_tag="OO130_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000626493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HCR5367270.1"
                     /translation="MISVLNPVLTRNAAGEMTEEWVSCGKIHADIRGRSSRERMQSGA
                     EMAQAEIRIWVRGQSGREITAASRLHVLSGPWRDRILNVVGLPVPDATGGRLEILCRL
                     GGEK"
     gene            complement(1539..1865)
                     /locus_tag="OO130_004080"
     CDS             complement(1539..1865)
                     /locus_tag="OO130_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HCR5367271.1"
                     /translation="MTALLTLEEIKAHLRVDHDADDDMLMDKVRQATAVLLAYIQGSR
                     DKVLREDGELIPGEALTRMKGAAMRLTGMLYRNPDLAEREELLQGELPFSVSVLIYDL
                     RCPTVL"
     gene            complement(1862..2461)
                     /locus_tag="OO130_004081"
     CDS             complement(1862..2461)
                     /locus_tag="OO130_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001674594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HCR5367272.1"
                     /translation="MPAGKSLTPDDVRPMLEQMVTEAVSHIPVPRDGRDYDPDVLQKA
                     VLDAVSALPAPQDGRDATALEILPAIDVQKSFPRGTYATHQGGLWRAYEKTHGMRGWE
                     CLVDGVADIDVSMTGERLFSVVVRQSSGQRTEKTFSLPVMLYRGVFRAGETYHPGDTV
                     TWGGSLWHCNSMTEDKPREAHSSAWTLAAKRGRDAGGGK"
     gene            complement(2452..3103)
                     /locus_tag="OO130_004082"
                     /pseudo
     CDS             complement(2452..3103)
                     /locus_tag="OO130_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
BASE COUNT          671 a          971 c          770 g          691 t
ORIGIN      
        1 ttaaagccct taccggacgg aaacagcacc ttaaacgcat acaacgcgtc attgtcatag
       61 gcatcacgca gggcgttctg ggcctgattc agataaaaat tacccgacat ggaaatctca
      121 gacgacgccc ccagaccgtt gatgttctcc tgctctgtgg agcagagcgt ggtcacatca
      181 atatcctgtt tctgaccggc ggtgaactgg acttccttga tggtgcagtc caggcgcaga
      241 tattccgcct tatccatagt ttcagcagtc gccggggcag atgaaatcat cacctgcgtc
      301 agctgtgagc gttcatacaa agcagacatt ctgcctcctg ataataaaaa acccgcacgc
      361 ggcggggtat ggttttgtag aaaaaaagaa aaagtcacac cgtgacctga aactccaggg
      421 ttgcacggta acagcggttt tccggaatat agtcctgcat ttcactgacg gatcccgggg
      481 ccagcagcat tatggcttca cgggcgtcct gacgtatctg acgcgcctgc gtcacagtcc
      541 cggcataaac gtctatctgc accgacactg aggactccgc ctgcccgccc atcacgtccg
      601 ctgacaccga tgaaatcagg ctgaaaacca cccacggaag cgccaccgac ggcctgccat
      661 ccagcagggg ggccacatac ggggacacct gcccgccggc aagatgcgcc agatgaggat
      721 acaaatccgc ctccgtcatc gtctcagtac ctcatcaatg gcccggttca tccgcgcaat
      781 cgccacctga gctgcctgtt cactgcgcac atcaaacgcc gggcgcacaa acgggtgcgg
      841 tggcatattc acggtcccca tttccacaaa ccgccagtag aaagcattgc gcgggttatc
      901 cgccttcatg gtgttatcgc tgttaccggt gtccggatta acaccacgga tatggacacc
      961 ggattccatc ccgccatcgc gggagcgccg ggaaaggatc accacattgc ggcgcagttt
     1021 tcccctgcgt accggtgtcc gtgacaccac ttcttctttc agcacattcg cacccgcacg
     1081 ggttgcctca cgcagcaccc ggttattttc cgcaccactc agaagctgca aatcgcggct
     1141 gatgtcctcc agccccgaaa aatccagcag gatttcgatc atttttcccc tcccagccga
     1201 cagagaattt ccagacgccc gccggtcgca tccggcacgg gcagcccgac aacgttcagg
     1261 atccggtcac gccatggacc actcagcaca tgaagtcgtg acgctgccgt gatttcccga
     1321 ccggactgac cgcgcaccca gatgcggatt tccgcctgcg ccatttccgc accggactgc
     1381 atccgctccc ggctgctcct gcctcggata tccgcatgaa ttttcccgca tgacacccat
     1441 tcttccgtca tttctccggc agcgttacgg gttaacaccg ggttcagaac acttatcatc
     1501 tatgtcagac gacctgcaga tattgccatt ccccctcctc ataacaccgt cggacaacgc
     1561 aaatcgtaaa tcagcacgga aacagaaaac ggcagctccc cctgaagcag ttcttcccgc
     1621 tccgcaagat ccggattccg gtacagcatc ccggtcagtc gcatggcagc ccccttcatc
     1681 cgggttaatg cctcgcccgg gatcagttca ccgtcctcac ggagcacttt atcccggctg
     1741 ccctgaatgt aggccagcag cacggcggta gcctgacgaa ccttgtccat cagcatgtca
     1801 tcatccgcgt catggtcgac acgcagatgt gccttgatct cttccagtgt cagtaatgcc
     1861 gtcattttcc gcctcctgca tcccgcccac gttttgcagc cagggtccag gctgatgaat
     1921 gagcttctcg gggtttatct tcggtcatac tgttgcagtg ccacagcgag cccccccacg
     1981 tcaccgtatc gccggggtgg taggtttcac cggctctgaa cacaccgcgg tagagcatca
     2041 ccggcaggga aaatgttttt tccgtacgct ggccactgct ctgccggacc accacagaga
     2101 acaaccgctc acccgtcatg ctgacgtcaa tatccgccac cccgtcaacc aggcattccc
     2161 atccccgcat cccgtgcgtt ttttcatacg cccgccagag tccgccctgg tgtgtggcat
     2221 acgtgccccg gggaaaggat ttttgaacgt caatggcggg gagtatttcc agagccgtgg
     2281 catcacgccc gtcctgcgga gccggcaggg cactcaccgc atccagaacc gccttctgca
     2341 gaacatccgg atcgtagtca cgaccatcac gcggaacagg gatatggctt acagcctccg
     2401 tcaccatctg ttcaagcatc ggacgcacat catccggggt gagactttta ccggcaggga
     2461 tgctttcacc atatacgaac cgtttctttc gggcgtcatg gtgacctcgg ctacaccctg
     2521 cgtattagta gagaccgtat tctggctgat gatcatttgc gaggatggct ctgcactgaa
     2581 tgtcacgggt tgatgcgcaa ccgggttgcc aaactggtcg ttaacgtgag ctttaactgc
     2641 tacgtcttgt gcgaccatta cctgaccttc cggtatctcc agactggtaa tcgtcgcaga
     2701 attaacatct gcactggcat tcagtaatcg cgagatgact tcagtaggtt tatctgccag
     2761 agaggctgaa actgttttca gtccgacctc ggtgcttgtc acaagaattt cagcgaaacc
     2821 ccgatcatcc gtttcaacgc tggtgctgct tagcgtgacg gaggttccgc ggaaattaac
     2881 ttttatgcct tctaccgggt tgccgttagc gtcagtgacg cttgccgtca tggttgcgcc
     2941 ctcggtcgtg ctgacgacga atgagtatac agaggttaag gaggctagtt ttgcggtacc
     3001 agcatcggca atcaaagtca cctgtttcgt atcactgaca ccataattgt tcacgctaac
     3061 tgtcacgggg taggtgccgg attttgtacc gctcagtgta act
//
LOCUS       DAJTMC010000267         3067 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.271, whole genome shotgun sequence.
ACCESSION   DAJTMC010000267 DAJTMC010000000
VERSION     DAJTMC010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3067
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.271"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(51..235)
                     /locus_tag="OO130_004083"
                     /pseudo
     CDS             complement(51..235)
                     /locus_tag="OO130_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311258.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4102 domain-containing protein"
     gene            complement(397..471)
                     /locus_tag="OO130_004084"
     tRNA            complement(397..471)
                     /locus_tag="OO130_004084"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(436..438),aa:Arg,seq:cct)
     gene            complement(547..1479)
                     /locus_tag="OO130_004085"
     CDS             complement(547..1479)
                     /locus_tag="OO130_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HCR5367273.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETHDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            1773..2528
                     /gene="mlaA"
                     /locus_tag="OO130_004086"
     CDS             1773..2528
                     /gene="mlaA"
                     /locus_tag="OO130_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HCR5367274.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            complement(2710..3045)
                     /locus_tag="OO130_004087"
     CDS             complement(2710..3045)
                     /locus_tag="OO130_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001315756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5367275.1"
                     /translation="MAESFATGSDHQVVNELNGERLREPNEVFKRLGRAIRYNFQVDD
                     AKFRRDNVKEIISTLFANKVDVDHPENKYKDFKDLEDKVEKRLQNRQTEYQNEINQLS
                     APGVNFDDI"
BASE COUNT          767 a          709 c          711 g          880 t
ORIGIN      
        1 cccatctttt acggggtgaa ggtagatttc gtatgaaggg tatctgttgt ctcctgcaaa
       61 gtcataagcg gatattttcc tacggtcagt attttttcct taccgtcaat cttgtagcga
      121 agttgccata ccttttcccg gatacaggaa cataaaggta caggccatta ccatcgagaa
      181 ggcggtatgg tttttctttc ggctttgctg cttcaatctg cttaacggtg agcatgggta
      241 aaaatccggt gggtaaaatt attttatcca ctttttaccc gtcatggagt gcggctgtca
      301 acgatctgac gcgaaccatg acgaaccgtg aatatacgga aggcttgata ttcaggggat
      361 tttgcggact ggtacggatg ggagcgaact gataaatggt gtcccctgca ggaatcgaac
      421 ctgcaattag cccttaggag gggctcgtta tatccattta actaagagga caatgcggca
      481 tgagtatacc cgctaatgga ctgcggagta agtacactgc cgctcgattg cttaaaccct
      541 cgccatttat gctgggtttt tatcattttt cttaatgttt tccgcacgtt ctgctttttg
      601 gcgtgcttct gctttgcgct tgttgctcat gtcgttacgg atctgtgcat gactcattaa
      661 cgcgaagata aaggtgccgc cgcagatatt ccccgctaaa gtaggtagtg cgaagggcca
      721 aatgaaatcg ctccagtgca gcgtgccgtt aaacaccaga tagaggattt ctacagaacc
      781 gacaacgatg tgggtggtgt cacccagtgc aataagccag gtcatcaata taatcaccac
      841 aatctttgcc gcacccgctg caggaaacat ccaaaccata gtggcgatca gccagccgga
      901 aatgatcgcg ttggcaaaca tctcgctggg ggtgttcttc atcacatcca tgccgatttt
      961 gacaaatgca tcgtgagttt cttcattgaa gataggcata tattcaaatg cccatgcagc
     1021 aatacctgtc ccgagaatat tacccagcag cacgacgccc cataatcgca taagtaagcc
     1081 gacgttgctc attgtcggtt tttgcatgac gggtagtacc gcagtcacgg tgttttcggt
     1141 aaataattgc tggcgggcca taatgacgat aataaaacca aaggtataac cgagattctc
     1201 caacaagaag ctgcccggca cgccttccag ttcgacatga aatatccctt ttgccagtaa
     1261 cgaagcgccc atcgacagac ccgccgcaat ggctgaccac agtagtgcca ttgcgtcgcg
     1321 ttccagctct ttttcaccat cctggcggat atgttcatga attgccatcg cccgggaggg
     1381 gagtcgatct tcatctattt ctattttttt gccgcgctct ttttcttcgc tctcaacttc
     1441 aatttcgtcg ctgtgttgat caattttgtc gttgtccatg gtctcttctc ttgaattagc
     1501 acgtatagct aaagcgtagc ggcttttttg ctcgcaactg gcgggagtta ctctgaaaat
     1561 gtagaaaagg ccgcgtttgc ctttttctgt ttctatagaa tcaagtagcc tacagggcgg
     1621 cgattaccag gctatgatca aatcagcaaa tcagggcgtc tggacatcag ttgacgtgct
     1681 gttacaatcg cccacaccta aacaggcgga tacggtatcg ttccgtcatg gatggcaaac
     1741 tgcataagcc ataaaaaaac agggagacat ttatgaagct tcgcctgtcg gcgcttgctc
     1801 tgggaactac gcttctggtg gggtgtgcga gttccggtac agatcagcaa gggcgttctg
     1861 acccgttaga agggttcaac cgcaccatgt acaacttcaa cttcaatgta ttagacccgt
     1921 atattgttcg accggtcgct gtcgcctggc gtgattatgt tccgcaaccg gcgcgtaacg
     1981 gtttgagcaa ctttaccggc aaccttgaag aacctgcggt gatggttaac tacttcttgc
     2041 agggcgaccc ttatcagggg atggtccact ttacccgctt tttcctgaac accattttgg
     2101 ggatgggcgg ctttattgat gttgcaggga tggcgaaccc gaaactgcaa cggactgaac
     2161 ctcaccgctt cggtagtacg cttggtcatt atggcgtggg ttatgggcct tacgttcagt
     2221 taccgttcta cggtagcttc acgctgcgtg atgacggtgg tgatatggca gatggtcttt
     2281 acccggttct ttcctggctg acctggccga tgtctgtggg taaatggacg cttgaaggga
     2341 tcgaaacccg cgctcagctg ctggattccg atggtctgct gcgtcagtcg tccgatcctt
     2401 atattatggt gcgcgaagcg tacttccagc gtcatgattt catcgctaat ggcggcgaac
     2461 tcaaaccgca ggaaaatccg aacgcacaag cgattcagga tgatttaaaa gatattgatt
     2521 ctgaataaga aacaaataaa aaaggtgagt cgcaaaactc accttttttg ttgttatccc
     2581 tcaattacca caagtaaaag gagataaacc gacaatgacc cggacgtaat gatgtaaaaa
     2641 tactgatgtt cgtcactgac tatttccgat attcaggcgt tgaaaaatca aaaaactgga
     2701 aaaataattt taaatatcat caaaattaac acctggcgca gatagttgat taatttcatt
     2761 ttgatattcc gtctggcgat tttgaagcct tttttcaact ttatcttcga gatccttaaa
     2821 gtctttgtat ttattttcag gatggtcaac gtcaactttg ttagcgaata aagtagaaat
     2881 tatttctttt acattatcgc gtctaaattt tgcatcgtcc acttgaaaat tatatcttat
     2941 tgctcgacct aaacgtttaa aaacttcgtt aggttctctc agtctttccc cgttgagctc
     3001 gtttacaacc tgatgatcgg aacctgttgc aaatgattct gccatgatag ctttactgct
     3061 ttcaaac
//
LOCUS       DAJTMC010000268         2961 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.272, whole genome shotgun sequence.
ACCESSION   DAJTMC010000268 DAJTMC010000000
VERSION     DAJTMC010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2961
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.272"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            <1..96
                     /locus_tag="OO130_004088"
     CDS             <1..96
                     /locus_tag="OO130_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR5367276.1"
                     /translation="RLNAVLDYIDAVAATDTSTAPDVIWPELPEA"
     gene            complement(68..505)
                     /locus_tag="OO130_004089"
     CDS             complement(68..505)
                     /locus_tag="OO130_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR5367277.1"
                     /translation="MFYSPSLNIFVNPALKDDYINANSWPDDALAVSDDVYNEFAINT
                     PPDDKIRVAGKNGLPTWALIPPPSYEELIQQAESERQLLLNQANEYMNSKQWPGKAAI
                     GRLKGEELAQYNLWLDYLDALELIDTSSAPDIEWPTPPAVQAR"
     gene            complement(507..893)
                     /locus_tag="OO130_004090"
     CDS             complement(507..893)
                     /locus_tag="OO130_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001537786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5367278.1"
                     /translation="MPVSHYLCIFLNVGLGELSLAGTASGVIGLNGYVTIPLIISGSR
                     RTLIIQWGQARFGGSGGEDAGYLNDFPFAFPSACYGMIVSHVGHTPSGAGILSASAIT
                     SNQFRGFSSIATAANAVLGRYIAIGV"
     gene            924..1490
                     /locus_tag="OO130_004091"
     CDS             924..1490
                     /locus_tag="OO130_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016529756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR5367279.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNALNCAGCERIFEDKISGTKPDRPG
                     LKKLLRTLSAGDTLVVWKLDRLGRSMRHLVTLIEELRQRGVNFRSLTDSIDTSTPMGR
                     FFFHVMGALAEMERELIVERTRAGLAAARAKGRVGGRRPKLTTEQWAQIGRLLEAGES
                     RQRIALIFDVGVSTIYRKFPANESNESP"
     gene            1633..2805
                     /locus_tag="OO130_004092"
     CDS             1633..2805
                     /locus_tag="OO130_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath protein"
                     /protein_id="HCR5367280.1"
                     /translation="MAQDYHHGVRVVEVNEGTRSITTVSTAIVGMVCTGDDADAKMFP
                     LNKPVLITDVLTASGKAGESGTLARSLDAIADQAKPVTVVVRVPQGETEEETTTNIIG
                     AVTAEGKKTGMKALLSAQSQLGVKPRILGVPGHDTKAVATELLSVAQSLRGFAYLSAY
                     GCKTVQEAITYRENFSQREGMLIWPDFTGWDTVLNAEATAYATARALGLRAKIDEQTG
                     WHKSLSNVGVNGVTGISADVFWDLQDPATDAGLLNQNDITTLIRKDGFRFWGSRCLSD
                     DPLFAFENYTRTAQVLMDTMAEAHMWAVDKPLNPSLARDIIEGIRAKMRSLVSQGYLI
                     GGDCWLDESVNDKDTLKAGKLTIDYDYTPVPPLENLMLRQRITDQYLVNFSSQVSA"
     gene            2815..>2961
                     /locus_tag="OO130_004093"
     CDS             2815..>2961
                     /locus_tag="OO130_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HCR5367281.1"
                     /translation="MALPRKLKHLNLFNDGNNWQGIVESLTLPKFTRKYEKYRGGGMP
                     GAVDV"
BASE COUNT          769 a          735 c          772 g          685 t
ORIGIN      
        1 cgacttaacg ccgtgttgga ttacattgac gcggtggcgg caacagatac cagcaccgcg
       61 ccggatgtca tctggcctga actgccggag gcgtaggcca ttcaatatct ggagcactgg
      121 aggtatcaat cagttccagt gcgtccagat aatccagcca caaattatat tgcgccagtt
      181 cctcaccttt cagacgacca atagcggctt taccgggcca ttgtttactg ttcatgtatt
      241 cgttggcctg attaagcaat aattgccttt ctgattctgc ctgttgaata agttcttcat
      301 atgatggtgg aggtattagt gcccatgtgg gtaatccatt ttttcctgca acacgaattt
      361 tgtcatctgg gggcgtattg attgcaaatt cattataaac atcatcactg acagccagag
      421 catcatctgg ccatgaattt gcattaatgt aatcatcctt aagcgcagga ttcacaaaaa
      481 tgtttaaaga tggactataa aacatattaa acccctatag cgatataacg acctaataca
      541 gcatttgcag cagtcgctat gcttgaaaaa ccgcggaact gatttgatgt aattgcagaa
      601 gccgacagga ttcctgcgcc tgaaggtgta tgccccacat gactaactat catcccataa
      661 catgctgacg gaaaggcaaa aggaaaatca ttaagatatc ctgcatcttc accaccagac
      721 ccaccaaatc tcgcctgccc ccactgaata atcagtgttc tccgggaacc tgaaataatt
      781 aacggaatcg ttacataccc attcaggcca atgacacccg atgcagtgcc agccagagat
      841 aattctccca aaccaacgtt taagaaaatg cagagataat ggctaactgg catcatcccc
      901 ggtttttatt caggggattg atcatgctta ttggctatgt acgcgtgtca acaaatgacc
      961 agaacaccga tttgcaacgt aatgcactga actgcgcggg atgtgagcgg atttttgagg
     1021 ataaaatcag tggcactaag cccgacagac cggggcttaa aaaactgctc aggacactat
     1081 cggcaggaga cacgctggtt gtctggaagc tggacagact gggacgcagt atgcggcatc
     1141 ttgttacgct gatagaagag ttgcgccagc gtggtgtgaa tttccgaagc ctgactgaca
     1201 gtattgatac cagtacccca atgggccgtt tcttttttca cgtcatgggg gctctggctg
     1261 aaatggaacg tgaactgata gttgaacgta ccagagcagg gctggctgca gctcgtgcca
     1321 aaggcagagt aggtggacgc cgtcctaagt tgaccaccga acagtgggca cagattggac
     1381 gtttactcga ggccggagaa tcaagacagc gtattgcact gatttttgat gtgggcgttt
     1441 ctaccattta tagaaaattt ccggcgaatg agagcaatga atcgccctga gtcagtatta
     1501 ttttgatttt tcctgtaagc ggacaaatac cgtcattttg tgtgaataac gacacaactg
     1561 cgcttagctg tttgtcaggc acaatcactt caacataggg cgaagcctaa tccaatcagg
     1621 aggttcgcca ctatggctca ggattaccac cacggggtgc gcgttgttga agtcaacgaa
     1681 ggcacccgat ccattaccac ggtgagcacc gccatcgtgg gtatggtctg cacgggcgat
     1741 gatgccgatg caaaaatgtt tcctcttaat aaacctgtgc tgatcactga tgtgctgacc
     1801 gccagcggta aagcgggtga gtctggcacg ctggcccgtt cgctggatgc catcgctgac
     1861 caggcaaaac ccgtgaccgt tgttgtgcgt gtgccgcagg gtgaaacgga agaagaaacc
     1921 acaaccaata tcattggcgc agtgaccgcc gagggtaaaa aaacaggtat gaaagccctg
     1981 ttatctgccc agtcacagct cggcgttaaa ccgcgcattc tcggcgtgcc aggtcacgac
     2041 acgaaggcgg tagctactga gttgctgagc gtggcgcaaa gcctgcgtgg atttgcttac
     2101 ctgtcagcgt atggttgcaa gacggtgcag gaagcaatca cttaccgtga aaacttcagt
     2161 cagcgtgaag gaatgctgat ctggcccgac tttactggct gggacacggt gctgaatgcc
     2221 gaagcaacgg cttatgccac cgcccgtgcg ctcggtctgc gcgctaaaat tgatgagcag
     2281 accggatggc acaaaagcct gtccaacgtg ggcgtgaacg gtgtcaccgg aatttctgct
     2341 gatgtgttct gggatctgca ggacccggca accgatgcag gtctgcttaa ccagaacgat
     2401 atcaccacgc ttatccgcaa agacggtttc cgcttctggg gttcccgctg tctgagcgat
     2461 gatccgctct ttgccttcga aaactacacc cgcacggcgc aggtgctgat ggacacgatg
     2521 gcagaagcgc acatgtgggc ggtggacaaa ccgctgaacc cgtcgctggc ccgcgacatt
     2581 atcgaaggta tccgcgccaa aatgcgtagc ctggtcagtc agggctatct cattggtggt
     2641 gattgctggc tggacgagtc ggtgaacgat aaagacacgc tgaaagccgg gaaactcacc
     2701 atcgattacg actacacgcc agtgccgcca cttgaaaacc tgatgctgcg tcagcgcatc
     2761 accgatcagt acctggtgaa tttctccagc caggtcagcg cgtaagggga caacatggct
     2821 ttaccacgca aattaaaaca cctgaacctg tttaacgacg ggaacaactg gcaggggatc
     2881 gttgagtcgc tgacgctgcc gaaattcacc cgcaaatatg agaagtatcg cggcggcggt
     2941 atgcccggag cggtggatgt g
//
LOCUS       DAJTMC010000269         2941 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.273, whole genome shotgun sequence.
ACCESSION   DAJTMC010000269 DAJTMC010000000
VERSION     DAJTMC010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2941
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.273"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            576..1292
                     /locus_tag="OO130_004094"
     CDS             576..1292
                     /locus_tag="OO130_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5367282.1"
                     /translation="MNDNSLLRNSSLFIAYMGCVGWVSAYSYGWGTSFYYGFPWWVVG
                     AGLDDVARSLLYAIIVMGILFTGWGIGILFFLLIKKRSKIQDLSFFRLFFAITLLFFP
                     VIFELLILKQYFILPLSLSCIISSLVISIIIRIYGRIFSVSCFSDIPFVREHRIKLIM
                     AGFLVYFWLFSFLVGWYKPQLKKEYQMLCYNNSWYYVLARYDSRLVLSSSFKDDSNRF
                     LIFNTEQSGFYEINDVYVRK"
     gene            1844..2248
                     /locus_tag="OO130_004095"
     CDS             1844..2248
                     /locus_tag="OO130_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5367283.1"
                     /translation="MNTALIVALMCMWYAVPAAAKETLLAMPRNSTEHCYAEINVHGP
                     YGVYFRVVPHPPGGKSWVECNSDYYYSDKPPGVQILGTRAGCRVYGICGTTSTLHVAG
                     RGVVCIKNICSPRGMIIHRIRKRPVVAVSDEM"
     gene            2311..2941
                     /locus_tag="OO130_004096"
                     /pseudo
     CDS             2311..2941
                     /locus_tag="OO130_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          824 a          605 c          667 g          845 t
ORIGIN      
        1 tgtgggccat aaatattcca aaaaaatagc ccgctccgtt tattaccgga acgggcgaga
       61 gcatgacatt gtctgtaatt ataatgagac tgctgattct acttattttt aatttcaaga
      121 aaagcgtttt ctacaaagaa ttatgagatt taatgttttg tttagacaat attgtcattc
      181 tgttcaataa tgaaagtctt catcaacaac aagtaaaaac aacgtccctc tcatcgaacc
      241 ggacttgcag atttcccgca tccggctctc ttgcaacaca tcagaccttc acgttcgggc
      301 ggttatgggg aaggtccagc aagctgtatg agccctaact gtttgaatac cagtagatta
      361 ccagaaactt ctatcgattg aagacattac caactagcgt tactaaatgt tgaccaagtt
      421 ttgttcactt gtctggtttt gatagtagca ttctgcagag tattttgata tgataaatat
      481 gcattaacaa tgatattatt gttaatttcc tagcatgaaa attaaaagat caacttttta
      541 tctcgaatat tttttacatt atcagttggt ttcttatgaa tgacaattca ttgcttagaa
      601 attcttcatt attcatcgcc tatatgggat gtgtagggtg ggtcagtgct tattcttatg
      661 gctggggaac ctctttttac tatggatttc catggtgggt tgttggtgca ggtttggatg
      721 atgttgccag aagtttactc tatgctatca tagtaatggg tatacttttt accggttggg
      781 ggattggtat tttatttttt cttcttataa agaaaagaag taaaatacaa gacttgagtt
      841 tttttagact attttttgct attaccctac tgtttttccc tgtaatattc gaacttttaa
      901 ttctcaaaca atatttcata cttccattat cactttcatg cattattagt tcattggtta
      961 tttcaataat aattcgcata tatggacgga tcttttctgt atcatgtttt tcagatatcc
     1021 cttttgttag agaacaccgc attaagttaa ttatggctgg ttttttggtt tatttttggc
     1081 ttttttcctt tcttgttggc tggtataaac ctcaattaaa aaaagaatat cagatgcttt
     1141 gttataataa tagttggtat tatgttcttg ctcgttatga ttcacggctc gttttgtcta
     1201 gttcatttaa agatgacagt aacagattct taatatttaa tacagaacag tcgggatttt
     1261 atgaaatcaa tgatgtttat gtcagaaagt aataatcctc tcccacaaag tgaattaaac
     1321 gaatatataa tgcgttcgaa gtatatgtat tgagctgtca tactgagaaa caaaccttag
     1381 ggcagcacaa ttaaagccag aaacaactat gcaagaaagt tactaaataa gtgctcggaa
     1441 cagtaaaaca ctcagggggg actcagtcca gattatgtaa tcagataaat tgccaaacat
     1501 cacaaatcac cagcaggact gagtgatgcc gagcattgca ccaatacccc gtgatgaacg
     1561 atgcctgatg cagaaagctc cctataaaac aaagaacaaa aactacgacc ggcgattcac
     1621 cgtcatactg atgctgcatc gtggtgacac tgtctgtcac tttcatgttg actgaacaac
     1681 tctgctgcga agaaggtgta ccgccggcgg gatttgattg tccggcgtct agcttccact
     1741 tccctgatgg aactgaaagt tgttatggcc ggtcagcttg ccctgtccgc aggacatgag
     1801 gtgctgtgac ggacagcacc agtaacggaa agtgacaata aaaatgaata ctgcgctgat
     1861 agttgcacta atgtgtatgt ggtatgcggt acccgctgct gcaaaagaga ctttactggc
     1921 tatgccacgt aattcaacag aacattgcta tgcggagatt aatgtgcacg gaccatatgg
     1981 ggtgtatttc cgagtggtcc cgcatccccc cgggggaaag agctgggtcg aatgtaacag
     2041 tgactactat tactctgata aaccaccggg agtacagata ctcggaaccc gtgcagggtg
     2101 tcgggtatac ggaatctgcg ggaccacctc gacactgcat gtggctggaa gaggggttgt
     2161 gtgtattaaa aatatatgca gtccacgtgg gatgataatt cacaggatac ggaaacgtcc
     2221 tgtagtggct gtaagcgatg agatgtgatc ttatccagca atagtggaca cgcggctaag
     2281 tgagtaaact ctcagtcaga ggtgactcac atgacaaaaa cagtatcaac cagcaaaaaa
     2341 acacgtaaac agcattcgcc tgaattttgc agtgaagccc tgaagcttgc tgaacgcatc
     2401 ggtgttgctg ccgcagcccg tgaactcagc ctgtatgaat ctcagctcta tgcgtggcgc
     2461 agcaaactgc agcaacagat gacctcgtct gagcgtgaaa gtgaactggc cgctgaaaat
     2521 gcccgcctga agcggcaact ggcggtgcag gcagaggaac tggctatcct ccaaaaggcc
     2581 gcgacatact ttgcgaagcg cctgaaatga agtatgtctt tattgaaaaa catcaggctg
     2641 agttcagcat caaggcaatg tgccgcgcgc tccgggtggc ccgcagcggc tggtatacgt
     2701 ggtgtcagcg gcggacaggg ataagccctc gtcagcagtt ccgtcagcac tgcgacagcg
     2761 ttgtcctcgc ggcggccttt acccggtcaa aacagcgtta cggtgcacca cgcctgacgg
     2821 atgaactgcg tgctcagggt taccacttta acgtaaaaac cgtggcggca agcctgcgcc
     2881 gtcaggggct gagggcaaag gcctcccgga agttcagccc ggtcagttac cgcgcacatg
     2941 g
//
LOCUS       DAJTMC010000270         2920 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.274, whole genome shotgun sequence.
ACCESSION   DAJTMC010000270 DAJTMC010000000
VERSION     DAJTMC010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2920
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.274"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            <1..1324
                     /locus_tag="OO130_004097"
     CDS             <1..1324
                     /locus_tag="OO130_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5367284.1"
                     /translation="GLVVVFSWVFIFALYFTPYALIVGQLGSTFKDGKGGVSTWIKHT
                     MGPGLAYLAAWTYWVVHIPYLAQKPQAILIALGWAMKSDGSLIKEYSVVALQGLTLVL
                     FIFFMWVASRGMKSLKIVGSVAGIAMFVMSLLYVAMAVTAPAITEVHIATTNITWETF
                     IPHIDFTYITTISMLVFAVGGAEKISPYVNQTRNPGKEFPKGMLCLAVMVAVCAILGS
                     LAMGMMFDSRNIPDDLMTNGQYYAFQKLGEYYNMGNTLMVIYAIANTLGQVAALVFSI
                     DAPLKVLLGDADSKYIPASLCRTNASGTPVNGYFLTLVQVAILIMLPTLGIGDMNNLY
                     KWLLNLNSVVMPLRYLWVFVAFIAVVRLAQKYKPEYVFIRNKPLAMTVGIWCFAFTAF
                     ACLTGIFPKMEAFTAEWTFQLALNVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(1325..2233)
                     /locus_tag="OO130_004098"
     CDS             complement(1325..2233)
                     /locus_tag="OO130_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5367285.1"
                     /translation="MRMNMSDFATFFAVARNQSFRAAGDELGLSSSAISHSIKTLEQR
                     LKIRLFNRTTRSVSLTEAGSNLYERLRPAFDEIQIMLDEMNDFRLTPTGTLKINDARV
                     AARIFLMPLLVGFTREYPDIKVELTTDDSLVDIVQQGFDAGVRLSGIVEKDMISVAIG
                     PPVKLCVAATPEYFARYGKPRHPHDLLNHQCVVFRYPSGKPFHWQFAKELEIAVAGNI
                     ILDDVDAELEAVLMGAGIGYLLYEQIKEYLDTGRLECVLGDWSTERPGFQIYYPNRQY
                     MSCGLRAFLDYVKTGQISQSQRHRPQ"
     gene            2333..>2920
                     /locus_tag="OO130_004099"
     CDS             2333..>2920
                     /locus_tag="OO130_004099"
                     /EC_number="1.6.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H oxidoreductase"
                     /protein_id="HCR5367286.1"
                     /translation="MQSERIYLVWAHPRHDSLTAHIADAIHQRAMERKIQVTELDLYR
                     RNFNPVMTPEDEPDWKNMDKRYSPEVHQLYSELLEHDTLVVVFPLWWYSFPAMLKGYI
                     DRVWNNGLAYGDGHKLPFNKVRWVALVGGDKESFVQMGWEKNISDYLKNMCSYLGIED
                     ADVTFLCNTVVFDGEELHASYYQSLLSQVRDMVDAL"
BASE COUNT          739 a          621 c          729 g          831 t
ORIGIN      
        1 ggggctggtg gttgtttttt catgggtgtt tatctttgcg ctctatttca caccttatgc
       61 gctaattgtt ggtcagttag gctcgacctt caaagatggg aagggcgggg tcagtacctg
      121 gattaaacac acgatgggac ccggactggc ttatctcgcc gcgtggacct actgggtggt
      181 gcatattccc tatctggcac aaaaacccca ggcaattctg attgcgctcg gttgggcgat
      241 gaaaagcgac ggttcgctaa tcaaagaata ttcagtcgta gcgttacagg ggttaacgct
      301 ggtgctgttt atcttcttta tgtgggttgc ttcacgcggt atgaaatcgc tgaaaatcgt
      361 cggttctgtg gcagggattg cgatgtttgt tatgtcgctc ctgtatgtgg cgatggcggt
      421 aaccgcgcct gcgattactg aagtgcatat tgcgaccaca aacattacct gggaaacgtt
      481 cattcctcat atcgacttta cctacattac cactatttca atgctggttt tcgcggttgg
      541 cggagcagag aagatttctc cttacgttaa tcaaacgcgc aacccaggaa aagaatttcc
      601 aaaagggatg ttatgcctgg cggtgatggt tgcggtttgt gccattctgg gctcactggc
      661 gatggggatg atgtttgact cgcgtaatat cccggatgac ttaatgacta acggtcaata
      721 ttacgccttt cagaagctgg gcgagtatta caacatgggt aatactttaa tggtgattta
      781 cgccattgcg aataccctgg gacaagtagc tgcgttggta ttctcgattg atgccccgct
      841 taaagtgcta ttaggtgatg ctgacagcaa atatattcca gccagtttat gtcgtaccaa
      901 cgcttctggt acgcccgtta atggctattt tctgaccctg gtgcaggtgg caattctgat
      961 tatgctgccg actctcggca tcggtgatat gaacaatctc tataaatggc tgttgaatct
     1021 taactcggta gtgatgccgc tgcgttattt atgggtattt gttgcattta ttgcagtcgt
     1081 tcgcctggcg cagaaatata aaccagaata tgtctttatt cgtaataagc ctctggcaat
     1141 gaccgtcggg atttggtgtt ttgcctttac cgcctttgcc tgtttgacgg ggatcttccc
     1201 gaaaatggaa gccttcactg cagagtggac cttccagttg gcgctgaatg ttgcaacgcc
     1261 gtttgtgctg gtaggattag gactgatttt cccgctgctg gcgcgtaaag cgaatagtaa
     1321 ataattattg tggtcggtga cgctgactct ggctgatctg acccgttttt acataatcga
     1381 gaaatgcgcg caagccgcag gacatatact ggcggtttgg ataatagatc tggaagcctg
     1441 gacgctctgt gctccagtct cccagtacac attctaaacg accagtatca aggtactcct
     1501 tgatctgttc atagagcaga tacccgatac cagcacccat caaaacggcc tccagttcag
     1561 catcaacatc atccagaatg atatttcctg cgacagcaat ttccagctcc tttgcaaatt
     1621 gccagtggaa aggtttgccg ctgggataac gaaaaacaac acattgatga ttaagcagat
     1681 catgaggatg gcggggtttg ccatatcttg caaagtactc tggcgttgct gctacacata
     1741 atttcactgg cgggccgatc gccacgctga tcatatcttt ttcgacgatg ccactaagtc
     1801 gaacgccagc gtcaaagccc tgttggacga tatcgacaag agagtcgtca gttgtgagct
     1861 cgactttaat atcaggatat tcacgtgtga aaccaaccag taaaggcatt aaaaatatac
     1921 gtgcagctac ccgggcgtca ttgattttga gcgttcctgt cggtgtcaga cgaaaatcat
     1981 tcatctcatc taacatgatc tgaatttcgt cgaaagctgg gcgcaatcgt tcatagagat
     2041 tagaacccgc ttccgttaac gatacgcttc gggttgtccg attgaataag cgaattttaa
     2101 gacgttgttc cagtgtttta atactatggc taatggcgga cgaggataag cctaactcat
     2161 cgcccgctgc acgaaagctt tgattacggg ccacggcaaa gaaagtggca aagtcagaca
     2221 tattcatccg cattagtgaa aatccttcat taactcatcc tgaatcatca cgtttacagg
     2281 attatgccgc ctgcgtaaag tgcgctccag aacttaacgt ggaggtaaaa ttatgcagtc
     2341 tgaacgtatt tatttggtat gggcccatcc tcgtcatgat tcattgaccg cacatattgc
     2401 tgatgcgatc catcagcggg caatggagcg gaaaatacag gtgacggaac tcgatttata
     2461 tcggcgtaat ttcaacccag tgatgacgcc ggaagatgaa ccagactgga agaatatgga
     2521 taaacgttat tctccagagg ttcatcagct ttattcagag ctgcttgaac atgacacgtt
     2581 agtggtggtt tttcctctct ggtggtacag cttcccggca atgctaaaag gatatattga
     2641 cagagtatgg aataatgggc tggcttatgg agatgggcac aaattaccat tcaataaagt
     2701 tcgttgggtg gcgctggttg gaggagacaa agaatcattt gtccagatgg gctgggaaaa
     2761 aaatataagc gattatttga aaaatatgtg cagttatctt ggtattgaag atgccgatgt
     2821 cactttcttg tgtaatacag tggtattcga tggggaagaa cttcatgcga gctattatca
     2881 gtcgttatta tctcaggtac gggatatggt agatgcacta
//
LOCUS       DAJTMC010000271         2913 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.275, whole genome shotgun sequence.
ACCESSION   DAJTMC010000271 DAJTMC010000000
VERSION     DAJTMC010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2913
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.275"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            88..2604
                     /locus_tag="OO130_004100"
     CDS             88..2604
                     /locus_tag="OO130_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR5367287.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQASSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYTFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPRHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWDYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNEKNRNISVGVSGLQWGIGYSLNYQYSRYTDENNDRALSL
                     NLSIPLERWLPRSRVSYQMTSQKDRPTQHEMRLDGSLLDDGRLSYSLEQSLDDDNNHN
                     SSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAFALIDAN
                     GASGVRIQNYPGIATDPFGYAVIPYLTTYQENRLSVDTTQLPDNVDLEQTTQFVVPNR
                     GAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDDTGQQSIVDEGGILYLSGISS
                     KSQSWTVRWGNQADQQCQFAFNTPDSEPTTSVLQGTAQCH"
     gene            2615..>2913
                     /locus_tag="OO130_004101"
     CDS             2615..>2913
                     /locus_tag="OO130_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5367288.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQTGYYKL"
BASE COUNT          827 a          708 c          673 g          705 t
ORIGIN      
        1 ggctgcaatc aatgattatg gcggcagttc cgggacagaa actcgtccac tgcaataaca
       61 aatataaaaa acacaggtca tcagggaatg ccacaacgac accaccaggg acataaacgc
      121 acaccgaaac agttggcgct catcatcaaa cgctgtttgc cgatggtgct cactggcagc
      181 ggcatgcttt gcactaccgc taacgccgaa gagtattatt tcgaccccat tatgctggaa
      241 actacaaaaa gtggtatgca aacaactgat ctgtcacgtt tttcaaaaaa atacgcacaa
      301 ctaccaggaa cttatcaggt tgatatctgg ctgaataaaa agaaggtttc acagaaaaaa
      361 attacattta ccgccaatgc agagcaactt ctgcagccac agtttacggt agaacaacta
      421 cgtgagctgg gtattaaggt ggatgaaatc ccggcgctgg ctgaaaaaga tgacgatagc
      481 gtgatcaact cgcttgaaca aatcattccc ggtacagctg ctgaatttga tttcaatcat
      541 cagcgactta atttgagcat tccccaaatt gcactgtacc gtgatgcaag aggttacgtc
      601 tccccttctc gttgggacga tggtatacca acgctgttta ccaactactc gtttacaggt
      661 tctgataacc gttaccgcca gggcaatcgt agccaacgac agtacctgaa tatgcaaaat
      721 ggtgctaatt ttggcccctg gcgattacgt aactattcta cgtggacacg caacgatcag
      781 gcgtcaagct ggaacactat cagtagttat ttacaacgtg atatcaaggc gttgaagtct
      841 cagttgcttc tgggagaaag cgccaccagc ggcagtattt tttccagcta cacctttact
      901 ggcgtgcaac tcgcttccga cgataatatg ttgccaaaca gccagcgcgg atttgcccca
      961 acggtacgcg gtatcgcaaa cagtagtgca atcgtgacta tcaggcaaaa tggttatgtg
     1021 atctatcaaa gcaacgtgcc agcgggtgcc tttgaaatta acgatctcta cccctcttcc
     1081 aacagcggcg atttagaagt cacgattgaa gaaagtgacg gtacacaacg tcgctttatc
     1141 cagccttatt cttcattacc catgatgcag cgacctaggc atctaaaata tagcgcgacc
     1201 gctggacgct atcgcgctga tgcaaacagt gatagcaagg aacccgaatt tgctgaagcc
     1261 acggcaatat atggtttgaa taatactttt acgctgtatg gcggcctgct cggttctgaa
     1321 gattattatg cgctggggat cggtatcggc ggcacacttg gcgcactggg cgcgttgtcg
     1381 atggatatca acagagctga cacccaattc gataaccagc actcttttca tggctatcaa
     1441 tggcgtacgc agtacatcaa agatatcccg gaaaccaaca ccaatatcgc tgttagctac
     1501 tatcgctata ccaacgatgg ctattttagt tttgatgaag ccaatacccg caattgggac
     1561 tataacagtc gccaaaaaag tgaaattcaa ttcaacatca gccagacaat atttgatggg
     1621 gtaagtctgt atgcctccgg ttcgcagcaa gactattggg gcaataacga gaaaaacagg
     1681 aatatctctg ttggggtttc cggcctgcaa tggggaattg gttacagcct gaattatcaa
     1741 tacagccgct acactgatga aaataatgac cgtgcactct ctttgaatct cagtattccg
     1801 ttagaacgct ggttaccgcg tagccgggtt tcctatcaga tgaccagcca gaaagatcgc
     1861 ccaacccaac atgaaatgcg tcttgatggc tcactgctgg atgatggtcg cctgagctat
     1921 agcctggaac aaagtctgga tgacgataac aaccataaca gtagcctgaa cgccagttac
     1981 cgttcacctt atggaacctt cagtgccgga tacagttacg gtaatgacag cagccaatac
     2041 aattacggcg ttaccggcgg cgtggttatc catcctcatg gcgtgacgct ctcgcaatat
     2101 ctgggcaacg cttttgcgct tatcgatgct aacggggctt ctggcgtgag gatacaaaac
     2161 tatccgggga ttgctactga tccctttggc tatgcagtga ttccttatct cacgacttat
     2221 caggaaaacc gtctctcggt agatactacg cagctgcccg ataacgtcga tcttgaacaa
     2281 acaacacagt ttgtggtgcc caacagaggt gcaatggtag cggcgcgttt caacgccaat
     2341 atcggttatc gcgtacttgt tacagtcagc gatcgcaacg gtaaaccgtt gccctttggc
     2401 gctcttgcca gcaacgatga tacggggcaa caaagtatcg tcgatgaggg cggcatacta
     2461 tatctctctg ggatatcgag taaatcacaa agctggactg tacgctgggg aaatcaggca
     2521 gatcaacaat gtcagtttgc ttttaataca ccggattcag aaccaacaac ctctgtatta
     2581 caaggcacag cgcagtgcca ttaaggataa aaaaatgaaa agagcgcctc ttataacagg
     2641 acttttgttg atatccacat cctgcgctta tgcctcctca ggagggtgtg gagccgacag
     2701 cactagcggt gcgacaaatt acagcagtgt ggttgatgat gttacggtga accagacaga
     2761 taacgtgaca ggacgggagt ttacctctgc aacgctaagt agcactaact ggcaatacgc
     2821 ctgttcctgc tctgcgggta aggcagttaa acttgtctat atggtcagcc ccgtacttac
     2881 caccactgga catcagacag gatattacaa act
//
LOCUS       DAJTMC010000272         2890 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.276, whole genome shotgun sequence.
ACCESSION   DAJTMC010000272 DAJTMC010000000
VERSION     DAJTMC010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2890
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.276"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            <1..2750
                     /locus_tag="OO130_004102"
     rRNA            <1..2750
                     /locus_tag="OO130_004102"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          763 a          638 c          904 g          585 t
ORIGIN      
        1 aactgaatcc ataggttaat gaggcgaacc gggggaactg aaacatctaa gtaccccgag
       61 gaaaagaaat caaccgagat tcccccagta gcggcgagcg aacggggagc agcccagagc
      121 ctgaatcagt gtgtgtgtta gtggaagcgt ctggaaaggc gtgcgataca gggtgacagc
      181 cccgtacaca aaaatgcaca tattgtgagc tcgatgagta gggcgggaca cgtggtatcc
      241 tgtctgaata tggggggacc atcctccaag gctaaatact cctgactgac cgatagtgaa
      301 ccagtaccgt gagggaaagg cgaaaagaac cccggcgagg ggagtgaaaa agaacctgaa
      361 accgtgtacg tacaagcagt gggagcacgc ttaggcgtgt gactgcgtac cttttgtata
      421 atgggtcagc gacttatatt ctgtagcaag gttaaccgaa taggggagcc gaagggaaac
      481 cgagtcttaa ctgggcgtta agttgcaggg tatagacccg aaacccggtg atctagccat
      541 gggcaggttg aaggttgggt aacactaact ggaggaccga accgactaat gttgaaaaat
      601 tagcggatga cttgtggctg ggggtgaaag gccaatcaaa ccgggagata gctggttctc
      661 cccgaaagct atttaggtag cgcctcgtga attcatctcc gggggtagag cactgtttcg
      721 gcaagggggt catcccgact taccaacccg atgcaaactg cgaataccgg agaatgttat
      781 cacgggagac acacggcggg tgctaacgtc cgtcgtgaag agggaaacaa cccagaccgc
      841 cagctaaggt cccaaagtca tggttaagtg ggaaacgatg tgggaaggcc cagacagcca
      901 ggatgttggc ttagaagcag ccatcattta aagaaagcgt aatagctcac tggtcgagtc
      961 ggcctgcgcg gaagatgtaa cggggctaaa ccatgcaccg aagctgcggc agcgacgctg
     1021 atgcgttgtt gggtagggga gcgttctgta agcctgtgaa ggtgtgctgt gaggtatgct
     1081 ggaggtatca gaagtgcgaa tgctgacata agtaacgata aagcgggtga aaagcccgct
     1141 cgccggaaga ccaagggttc ctgtccaacg ttaatcgggg cagggtgagt cgacccctaa
     1201 ggcgaggccg aaaggcgtag tcgatgggaa acaggttaat attcctgtac ttggtgttac
     1261 tgcgaagggg ggacggagaa ggctatgttg gccgggcgac ggttgtcccg gtttaagcgt
     1321 gtaggctggt tttccaggca aatccggaaa actaaggctg aggcgtgatg acgaggcact
     1381 acggtgctga agcaacaaat gccctgcttc caggaaaagc ctctaagcat caggtaacat
     1441 caaatcgtac cccaaaccga cacaggtggt caggtagaga ataccaaggc gcttgagaga
     1501 actcgggtga aggaactagg caaaatggtg ccgtaacttc gggagaaggc acgctgatat
     1561 gtaggtgaag tccctcgcgg atggagctga aatcagtcga agataccagc tggctgcaac
     1621 tgtttattaa aaacacagca ctgtgcaaac acgaaagtgg acgtatacgg tgtgacgcct
     1681 gcccggtgcc ggaaggttaa ttgatggggt cagcgcaagc gaagctcttg atcgaagccc
     1741 cggtaaacgg cggccgtaac tataacggtc ctaaggtagc gaaattcctt gtcgggtaag
     1801 ttccgacctg cacgaatggc gtaatgatgg ccaggctgtc tccacccgag actcagtgaa
     1861 attgaactcg ctgtgaagat gcagtgtacc cgcggcaaga cggaaagacc ccgtgaacct
     1921 ttactatagc ttgacactga acattgagcc ttgatgtgta ggataggtgg gaggctttga
     1981 agtgtggacg ccagtctgca tggagccgac cttgaaatac caccctttaa tgtttgatgt
     2041 tctaacgttg acccgtaatc cgggttgcgg acagtgtctg gtgggtagtt tgactggggc
     2101 ggtctcctcc taaagagtaa cggaggagca cgaaggttgg ctaatcctgg tcggacatca
     2161 ggaggttagt gcaatggcat aagccagctt gactgcgagc gtgacggcgc gagcaggtgc
     2221 gaaagcaggt catagtgatc cggtggttct gaatggaagg gccatcgctc aacggataaa
     2281 aggtactccg gggataacag gctgataccg cccaagagtt catatcgacg gcggtgtttg
     2341 gcacctcgat gtcggctcat cacatcctgg ggctgaagta ggtcccaagg gtatggctgt
     2401 tcgccattta aagtggtacg cgagctgggt ttagaacgtc gtgagacagt tcggtcccta
     2461 tctgccgtgg gcgctggaga actgaggggg gctgctccta gtacgagagg accggagtgg
     2521 acgcatcact ggtgttcggg ttgtcatgcc aatggcactg cccggtagct aaatgcggaa
     2581 gagataagtg ctgaaagcat ctaagcacga aacttgcccc gagatgagtt ctccctgact
     2641 ccttgagagt cctgaaggaa cgttgaagac gacgacgttg ataggccggg tgtgtaagcg
     2701 cagcgatgcg ttgagctaac cggtactaat gaaccgtgag gcttaacctt acaacgccga
     2761 aggtgttttg gcggatgaga gaaagatttt cagcctgata cagattaaat cagaacgcag
     2821 aagcggtctg ataaaacaga atttgcctgg cggccgtagc gcggtggtcc cacctgaccc
     2881 catgccgaac
//
LOCUS       DAJTMC010000273         2868 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.277, whole genome shotgun sequence.
ACCESSION   DAJTMC010000273 DAJTMC010000000
VERSION     DAJTMC010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2868
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.277"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(<1..1075)
                     /locus_tag="OO130_004103"
     CDS             complement(<1..1075)
                     /locus_tag="OO130_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000142995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HCR5367289.1"
                     /translation="MAFKHYDVVRAASPSDLAEKLTHKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGAVISATSDPEYYFVVVLAGQSNGMSYGEGLPLPGTYDRPDPRIKQLARRST
                     VTPGGAACKYNDIILADHCLHDVQDMSRLNHPKADLSKGQYGTVGQGLHIAKKLLPFI
                     PANAGILLVPCCRGGSAFTTGADGTYSDASGASENSTRWGVDKPLYKDLIGRTKAALE
                     KNPKNVLFAVVWMQGEFDFGGTPANHAAQFGALVDKFRADLADMAGQCVGGSAGGVPW
                     ICGDTTYFWKQKNESTYQTVYGSYKNKTEKNIHFVPFMTDENGVNVPTNKPEEDPDIR
                     GIGYYGSKWRDSSATWTSQDRASH"
     gene            complement(1565..1641)
                     /locus_tag="OO130_004104"
     tRNA            complement(1565..1641)
                     /locus_tag="OO130_004104"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1605..1607),aa:Arg,seq:tct)
     gene            complement(1655..1731)
                     /locus_tag="OO130_004105"
     tRNA            complement(1655..1731)
                     /locus_tag="OO130_004105"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1695..1697),aa:Arg,seq:tcg)
     gene            complement(1739..1814)
                     /locus_tag="OO130_004106"
     tRNA            complement(1739..1814)
                     /locus_tag="OO130_004106"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1779..1781),aa:Ile,seq:cat)
     gene            complement(1855..2820)
                     /locus_tag="OO130_004107"
                     /pseudo
     CDS             complement(1855..2820)
                     /locus_tag="OO130_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001403604.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
BASE COUNT          648 a          851 c          691 g          678 t
ORIGIN      
        1 aatggctcgc cctgtcctgt gacgtccagg tggctgagct gtcacgccat ttcgaaccgt
       61 aataaccgat accccgaatg tccgggtctt cttccggttt gttcgtcggc acattcaccc
      121 cgttctcatc ggtcatgaac ggtacgaaat ggatattctt ttccgtttta tttttatagc
      181 tgccgtacac cgtctggtac gtggattcgt tcttctgctt ccagaaatac gtcgtgtccc
      241 cgcatatcca gggaacaccg ccagcagagc caccgacgca ctggcctgcc atatccgcca
      301 ggtctgcacg gaatttatca accagcgcac caaactgtgc ggcatgattt gccggcgtac
      361 cgccaaaatc aaattccccc tgcatccaca ccacggcaaa cagcacattt ttcgggttct
      421 tctccagtgc cgcttttgtt cgaccgataa ggtccttata cagcggcttg tccacacccc
      481 agcgggttga attctccgag gcaccactcg cgtcactgta tgtgccatca gctccggtgg
      541 tgaacgctga accaccacga cagcacggaa ccagcagaat gcccgcattc gccggtataa
      601 acggcagcag ttttttggcg atatgcagcc cctgccccac ggttccgtac tgcccctttg
      661 acaggtccgc tttcggatgg ttaagacggc tcatgtcctg cacatcatgc agacaatggt
      721 ccgccagaat gatgtcgtta tatttgcatg ctgcaccgcc cggtgtcacc gtactgcgac
      781 gcgccagttg cttaatacgc gggtccggac ggtcatatgt ccccggcagc ggaaggcctt
      841 caccatacga catgccgttt gactgccctg ccagaaccac aacaaagtaa tactccgggt
      901 cgctggtggc gctgattact gcaccttctg ctgcaatcgc ctgcatcaga gtataagggg
      961 ttatggccac cggactacca aacggctgcc agccctcttt cagtttatgt gtcagctttt
     1021 ccgcaagatc tgacggcgac gccgccctga caacatcata gtgtttaaaa gccatgaatc
     1081 ctcccggccg ggataatatt gtgagtaaaa tgaggagcgg gctgaagtcc ggaagttaca
     1141 ggacaatggc agaagagaga cgacagcccg caattcgaaa aagaccgcgc agttgcgcag
     1201 agtgactact atggggtatt attcgccagc tgaaatatta cttcacgttt tattgtttat
     1261 tccttgccgc ccgcgtctcc cagcgcgggc tttttttgcc cacaagaaag cccctccgga
     1321 gaggggctgg agagtggcgc tatgtgccat tgcatggtgc cgggtgcctc ccggtgaatt
     1381 cagtaccagc acctgaatcc gcgattatcc catataccta ctcgctgatt gcccctccgc
     1441 acagggggat tcaccatgcc agtttctttt aacaaactcc ccgcaaacca gacaacagtc
     1501 aaccgtctga attgtgagac atttaaaaaa aaagcccgca aaagcgagcc agggaaaata
     1561 agtgtggcgc gttgtactgg attcgaacca gtgaccgatt gcttagaagg caattgctct
     1621 gtccggctga gctaacaacg catgatgcag ataatggacc gccatcgggg acttgaaccc
     1681 cgcgcagcca gcttcgaagg ctggcgctct ttcctgatga gctaatggcg gtatgtgatg
     1741 gtggcccttg ctggatttga accagcgacc tggcgattat gagtcgctcg ctctcaccac
     1801 tgagctaaag ggccgggagc cgcataataa cgacgcgtaa ttaattcttc aatatcatcc
     1861 gttctggctg actaaatcct gtacttcccg aaccgtctgc tcaaaacgtt cagtctccag
     1921 ctcaacgcca attgcacaac gcccgagcgc cagtgccgct ttcactgtcg aacccgaccc
     1981 catgaaaaaa tctgcaatca ggtcacccgg acgactgcta gcgctgatta tctgctgcag
     2041 catttctgcc ggtttttcgc acggatgttt cccgggatag aactgcaccg gtttatgcgt
     2101 ccacacatcg gtatacggca cctgcaccgt cacgccaaaa taccgccgca gatgtttata
     2161 ttcactcagc agctccgcat actgccggtt cagtgaggta tacgtatcca ccagctggtg
     2221 gtggagcttt tccagttcac cacgccgatg cttctcttct gccacccggg caaacagcgc
     2281 ctgtaatttc agataatcgc tttcattcgg caactgccac tgactggcac tgaaccagtg
     2341 cgacaccatg tttttctttc ctgtggcatc tgcaatctgt tttgccgtta tccccagggc
     2401 agctcgcgca tcacgaaagt aagaaatcag cggggccatc acatgctgtt tcagtgcact
     2461 gcccttcgcc tcatagccat catctttcgg gcggtacggc ccctgataat gttccgcgaa
     2521 cagaatgcgc tctgtggcgg ggaaatacgc ccgcaggctt tccttgttgc atccgttcca
     2581 gcgtccggac ggcttcgctc agataatatg gttcagcaca ctgaagcgtt cacgcatcat
     2641 gatttcgata tcagatgcca ggcgatgacc acagaacagg taaagacttc cggcaggttt
     2701 cagcacccgc cagaactgcg ccagacactg ctccagccac ttcaggtaat catcgtcgcc
     2761 cttccactgg ttatcccagc cctcaggctt cactttaaag tacggcgggt ccgtgactat
     2821 caggtcaaca gaattttcgg gtaacgacca gataaattcc aggcagtc
//
LOCUS       DAJTMC010000274         2862 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.278, whole genome shotgun sequence.
ACCESSION   DAJTMC010000274 DAJTMC010000000
VERSION     DAJTMC010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2862
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.278"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            <1..278
                     /locus_tag="OO130_004108"
     CDS             <1..278
                     /locus_tag="OO130_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR5367290.1"
                     /translation="KYEGEQLYLNELGYVYGNEEKTNEIVNNNPGYVVEIFEEKMGNE
                     LKIRTRMMEILRDGKINICEYINKEQLEKLNPPEDLRIAIKKLGWNN"
     gene            complement(350..1354)
                     /gene="argF"
                     /locus_tag="OO130_004109"
     CDS             complement(350..1354)
                     /gene="argF"
                     /locus_tag="OO130_004109"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HCR5367291.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIAETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     "
     gene            1516..1932
                     /gene="rraB"
                     /locus_tag="OO130_004110"
     CDS             1516..1932
                     /gene="rraB"
                     /locus_tag="OO130_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HCR5367292.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            complement(1978..2480)
                     /locus_tag="OO130_004111"
                     /pseudo
     CDS             complement(1978..2480)
                     /locus_tag="OO130_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026287.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            2673..>2862
                     /locus_tag="OO130_004112"
     CDS             2673..>2862
                     /locus_tag="OO130_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 family protein"
                     /protein_id="HCR5367293.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQ"
BASE COUNT          792 a          633 c          658 g          779 t
ORIGIN      
        1 agaagtatga gggcgaacag ctgtatctta atgagttagg atatgtttat ggtaatgaag
       61 aaaaaacaaa tgaaattgta aataacaatc cgggttatgt cgttgagatt tttgaagaaa
      121 agatgggtaa tgaattaaaa attagaacaa gaatgatgga aatcctccgg gatgggaaaa
      181 taaatatatg tgaatatatt aataaagaac agttagaaaa attaaatcct ccggaagatt
      241 tacgtatagc aataaaaaaa ttggggtgga ataattgaac tattactgca taaaataatg
      301 tgatgccggg atgtttttgt atttcccggc atctttattg cgatatcaat tatttactga
      361 gcgtcgcgac catcaccgct ttgatggtat gcatgcggtt ttccgcctga tcaaaaacaa
      421 tgctggcggc agattcgaag acctcatcag tcacttccat tccgccatgt aggccaaact
      481 cttccgccat tttcttacca agcgtcgtct ggtcgtcatg aaacgcgggc aggcagtgga
      541 ggaatttgac ctccgggtta ccggtcaact gcatcatctt gctgttcacc tgataatcac
      601 gcagtaatgc aatccgttct gcccatttct cttttgcttc ccccatcgac acccatacat
      661 cggtatagat aaagtcagca ccttcaactc ctttggctac atcttccgtc agcgtaatat
      721 tcccgccatt ttgctgtgcc aaggcgcggc attccgtaac cagcgcagct tccggccagc
      781 atgcctgtgg cgcgaccaga cgcaaatcca gaccggtaag cgccgcagct tcgagcatcg
      841 aattgcccat gttgttacgt gcgtcacctg catagaccag cgtcatttcg ttgaatgctt
      901 taccgggtaa atgctcctgc atggtgagga gatccgccag caactgcgtg gggtgaaact
      961 cattggtcag gccgttccat accggcacgc cagcgtattc cgccagtgtt tcggcaatct
     1021 cctgaccata gccgcgatac tgaataccgt catacatgcg acccagcacg cgggcagtgt
     1081 ctttaatcga ctctttatga ccaatctgac tgccgcttgg gccgagataa gtaacgcgag
     1141 caccctggtc atatgcggca acttcgaaag agcatcgggt acgggtcgag tctttttcga
     1201 agatgagcgc gatgttttta ccagtgagtt tggcttcttc tttgccgctt ttcttatcgg
     1261 ctttcagctt cgcggctaac tgcagcaggc tgttgagttc agctggcgtg aaatcgagta
     1321 atttcaggaa atgcttatga taaaacccgg acatagatcc ctcctgtggc taacgcctca
     1381 atgaattaaa attcaattta tatggatgat tattcatttg caagtctaaa gcataaatct
     1441 ttgtcacaaa ggtggaggca atgtcagtgg tatgtgacaa taagagtatc ggcaggacat
     1501 taagaggaat gagccatggc aaacccggaa caactggaag aacagcgtga agaaactcgt
     1561 ttgattattg aagaattact ggaagatggc agcgatccgg acgcgctgta caccatcgaa
     1621 caccatcttt ccgcagacga tctggaaacc ctggaaaaag cagcagttga agcgtttaaa
     1681 ctcggttacg aagtgaccga tccagaagag ctggaagttg aagatggtga tatcgtgatt
     1741 tgctgcgaca tcctcagcga gtgcgcgttg aatgccgatc tgatcgatgc ccaggttgaa
     1801 caactgatga cattggcgga gaaatttgac gttgagtacg acggttgggg cacttatttt
     1861 gaagatccta atggcgaaga cggcgacgat gaagattttg tcgatgaaga cgatgacggg
     1921 gttcgccact aattaattga ttaaaatgcc cgatagcaca cactagcggg cattttttta
     1981 gagttcgcgc aacatccgca cttcacaatc aacatggccc gtgcagccaa gtgcatagtc
     2041 gatatgctca aagcccaaat gctcataaag cgcaatggct tcctttaaaa agcggtcgtt
     2101 tccagatagc agcgtttgaa gcccatctct cgtgcctgct ccatcgccat taaggccagt
     2161 tttttcgcca gccctttacc gcgaatagcc ggaagaaaat acatcttttg cagttcgcaa
     2221 atgtccgatt cactacctgc taatggcgca atcccaccgc cgccgaccac ttcaccctcg
     2281 tactcaacca cccaatatgc atgacccggc tgactataca cttgatacag ttcgtcaaga
     2341 ttcggatcgg cgacggtgta gcctttatca gcggtaagac cgtattcggc ggatacctga
     2401 cgaatgacgc gggcaatagc aggattatcc tgtagcgtca ggcgacgcat tacaggtgat
     2461 tgcggcgcaa tgttattcat agtttaaccc aataaaaata aagtaattat acatttgttt
     2521 aataccactc tcttcttacg tgcgcaagca tcttataaag caataaagtt tttgccagcc
     2581 tattactgat aagagtattg taacgtggca gaacatttta ctgtctgtat atataaatta
     2641 tatcaatgga ttttttaatt cagggaattt ttatggctca agttattaat gaaatggatg
     2701 ttccgtccca ttcgtttgtt tttcatggta caggtgagag atattttctt atttgtgtgg
     2761 tgaatgtgtt gttaacgatt ataacgctag gtatctattt accatgggca ttaatgaaat
     2821 gtaagcgtta tctctatgct aatatggaag ttaacggaca ac
//
LOCUS       DAJTMC010000275         2855 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.279, whole genome shotgun sequence.
ACCESSION   DAJTMC010000275 DAJTMC010000000
VERSION     DAJTMC010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2855
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.279"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            232..543
                     /locus_tag="OO130_004113"
     CDS             232..543
                     /locus_tag="OO130_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCR5367294.1"
                     /translation="MFNVITHPAALDELLELPDNLRGRMTRLIERLEQEGNKMKMPHS
                     RVIGGGLFELRVGDKNIARTLYAYATGNEIYLLHAFVKKTQKTPTKAIEIARMRLKEM
                     N"
     gene            543..830
                     /locus_tag="OO130_004114"
     CDS             543..830
                     /locus_tag="OO130_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5367295.1"
                     /translation="MKVKGVPFSQVKQKLLSTPEAVKGYEDADKELEMVEMLYEMREK
                     AGLTKSALAERMGLRPSAISRLESNPLGASMKTLTKYAHACGATIDIHAVY"
     gene            complement(1367..2554)
                     /locus_tag="OO130_004115"
     CDS             complement(1367..2554)
                     /locus_tag="OO130_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HCR5367296.1"
                     /translation="MLPRFADIFQQGNRWLNWLEKQPEGAVRPVVIESVTKIMACGTT
                     LMGYTQWCCSSPDCCHTKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLVPDCPWQHIVF
                     TLPCQYWSLVFHNRWLLAEMSRIAADVILEICHQADVEPRIFTVIHTWGRDQQWHPHI
                     HLSTTAGGVTSGHTWKNLHFYARKVMSMWRYRITRLLSRKYPDLVMPDALAAEGSSKR
                     EWNRFLDTHYRRGWNVNVSRVMDNATHVAVYFGSYLKKPPVPVSRLEHYAGQDEIGLR
                     YNSHRTKREEYLLMSGDEFMERFSWHVADKGFRMVRYYGFLSPAKRRLLEEVVYIITE
                     TVRKTAMQITWRGMYQRLLKVDPLKCVLCGSQMRFTGLKRGYRLAEQVLMHEPLARMR
                     WCG"
     gene            complement(2554..>2855)
                     /locus_tag="OO130_004116"
     CDS             complement(2554..>2855)
                     /locus_tag="OO130_004116"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5367297.1"
                     /translation="VVKLHEDDFFDEEDAEVLRFDSFDDAVECCADLNIPFFVDAGNK
                     KLVFWFVRVDDEGYPEIARCTEREFATILSGISAGGMYCPECGTVHWPDGVAPPF"
BASE COUNT          739 a          770 c          665 g          681 t
ORIGIN      
        1 aaggtgaaat ctgtcaatca gtaccggctt acgccgggct tcggcggttt tactcctgtg
       61 ccatatgcaa caacggagtg ccgccttcca tgccgctaac gcggcatcaa aaaatccctc
      121 ttctccagtt gcccccctcc gaactcagag tgtattttca tttattgatg aaatttactg
      181 tttgcgcaat tttcctgttt tagtgtattt tgtcattctg gaggctataa tatgtttaat
      241 gtgattactc acccggccgc actggatgaa cttctagaat taccagacaa tctgcgtggt
      301 cgtatgacca gactgattga acggctggaa caggaaggta acaaaatgaa aatgcctcac
      361 agccgtgtaa ttggcggagg gctttttgaa ctgagagtcg gggataaaaa tatcgcaagg
      421 acactgtacg cttatgcaac gggtaacgaa atctatctgc tgcatgcatt tgtgaaaaaa
      481 acgcaaaaga caccaacgaa agctattgaa attgccagga tgcgtctgaa ggagatgaac
      541 tgatgaaagt aaaaggtgtt ccattcagcc aggtaaagca aaaactactc agtactccag
      601 aagctgtcaa agggtatgag gatgctgaca aagaactgga gatggtggaa atgttatacg
      661 aaatgagaga aaaagcggga ttaacaaagt cagcactggc cgaacgaatg gggttacgcc
      721 cgtcagccat cagtcgcctg gaaagtaacc ctttaggggc cagcatgaaa actttgacaa
      781 aatatgctca tgcctgtggc gcaaccatcg atattcacgc tgtctattga ggggtccgct
      841 tggaattcgg aaaataaacc acgttaagac tattttttag ataaatgact ttcaggaatc
      901 gccaagatgg cgggataaca gcggttttgg tgtcctaaat cgtacaattc tgtacatcca
      961 cgcaagggaa tgtacggaag taagccagac ggttatcgga cggtcagaac atttcgtaca
     1021 gcctttacgt tcagtgagtt agctggcctg gacgtaacca ctcgttagct caattctctt
     1081 agcgtgggaa aatttccgaa ttccaagcgg acccctttat ccttgccccg ctgaatgagg
     1141 aaacgctgtt ccgggggatt atgctgaacg tcttccgttc atcgatagga attaaaaccc
     1201 caaaaagatt aaaaaacacc acaaaacgga tgtttcttca acaccacttt tgctcatatg
     1261 aacggaaccg acgattaaac tggatggctc tgattgattc agggtatgaa tggcggtttt
     1321 ttgctccgtt tccctcaaaa tggacgctac ttaccctctg cggctctcag ccgcaccacc
     1381 gcatccgggc cagcggctca tgcatcagga cctgctctgc cagacggtag ccccgcttca
     1441 gccccgtaaa acgcatctga ctcccgcaca gcacgcactt cagcgggtca accttcagta
     1501 acctctgata catccctctc caggtgattt gcatcgccgt ttttctcact gtctccgtta
     1561 tgatgtacac cacttcttcc agtaaccgcc gtttcgccgg actcagaaaa ccgtagtacc
     1621 tcaccatacg gaacccctta tccgccacat gccaggagaa cctttccatg aactcatctc
     1681 cactcatcaa caggtattct tcccgttttg ttcggtgact gttgtaacgc agtccgattt
     1741 catcctgacc ggcataatgc tccagacgac tcaccggcac cggtggcttt ttcaggtaag
     1801 agccaaagta caccgccaca tgggtggcat tatccatcac ccgggatacg ttgacattcc
     1861 agccacggcg gtaatgcgtg tccaggaagc gattccattc ccgtttactg cttccttctg
     1921 ctgccagcgc atccggcatc accaggtcag ggtatttccg tgacagtaac cgtgttatcc
     1981 ggtagcgcca catgctcatc accttacggg cgtaaaaatg aagatttttc caggtgtggc
     2041 ccgacgtcac accaccggca gttgtcgata aatggatatg cggatgccac tgctggtcac
     2101 gcccccatgt gtggatcacc gtgaatatcc tcggctccac atctgcctga tggcagattt
     2161 ccagtatcac atccgctgca atgcggctca tctctgccag caaccaccgg ttgtggaaca
     2221 ccagggacca gtactggcag ggtaacgtga acacaatatg ctgccatggg cagtcgggga
     2281 ccaggctcag cagatactgt atccactgtg cgccagcctt caccccgcag tgcgggcagg
     2341 agcggctttt acaccggaag cagacctttt ttgtatggca acagtccggt gatgaacagc
     2401 accactgtgt ataccccatc agtgtggtcc cgcacgccat gattttggtc accgactcaa
     2461 tcaccaccgg acgtactgcc ccttccggct gcttctccag ccagttaagc cagcggtttc
     2521 cctgctgaaa gatatcggca aaacggggaa gcatcagaag ggcggggcga ctccgtccgg
     2581 ccagtgaacc gtgccacact ccgggcagta cataccgccg gcgctgatac cggaaagaat
     2641 ggtcgcaaat tcccgctccg tgcagcgggc aatttccgga tacccttcgt catcaacacg
     2701 tacaaaccag aagaccagtt ttttgtttcc ggcatccaca aagaacggaa tattcaggtc
     2761 tgcgcaacac tcaacggcat catcaaaact atcaaagcgc agaacttctg cgtcttcttc
     2821 gtcaaaaaaa tcatcttcgt gaagcttcac gacat
//
LOCUS       DAJTMC010000276         2840 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.280, whole genome shotgun sequence.
ACCESSION   DAJTMC010000276 DAJTMC010000000
VERSION     DAJTMC010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2840
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.280"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            1..134
                     /locus_tag="OO130_004117"
                     /pseudo
     CDS             1..134
                     /locus_tag="OO130_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
     gene            384..1250
                     /gene="ospB"
                     /locus_tag="OO130_004118"
     CDS             384..1250
                     /gene="ospB"
                     /locus_tag="OO130_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspB"
                     /protein_id="HCR5367298.1"
                     /translation="MNLDGVRPYCRTVNKKNESISDIAFAHIIKRVKNSSCTHPKAAL
                     VFLGEKGFCDSNDVLSIMGQQIPRVFKNKMLYDYVFKNEKSKNDFLKMAESWLPQSEP
                     IVINNDDDALNAAAYFSVKKAKIKTVNDTDFKEYNKVYILGHGSPGSHQLGLGSELID
                     VQTIISRMKDCGILNVKDIRFTSCGSADKVAPKNFNNAPAESLSCILNSLPFFKEKES
                     LLEQIKKHLENDESLSDGLKISGYHGYGVHYGQELFPYSHYRSTSIPADPEHTVKRSS
                     QKKTFIINKELD"
     gene            1580..2320
                     /gene="phoN2"
                     /locus_tag="OO130_004119"
     CDS             1580..2320
                     /gene="phoN2"
                     /locus_tag="OO130_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAP2 family phosphatase PhoN2"
                     /protein_id="HCR5367299.1"
                     /translation="MKTKNFLLFCIATNMIFIPSANALKAEGFLTQQTSPDSLSILPP
                     PPAEDSVVFQADKAHYEFGRSLRDANRVRLASEDAYYENFGLAFSDAYGMDISRENTP
                     ILYQLLTQVLQDSHDYAVRNAKEYYKRVRPFVIYKDATCTPDKDENMAITGSYPSGHA
                     SFGWAVALILAEINPQRKAEILRRGYEFGESRVICGAHWQSDVEAGRLMGASVVAVLH
                     NTPEFTKSLSEAKKEFEELNTPTNELTP"
     gene            complement(2673..2840)
                     /locus_tag="OO130_004120"
                     /pseudo
     CDS             complement(2673..2840)
                     /locus_tag="OO130_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="T3SS effector OspC family protein"
BASE COUNT          930 a          517 c          526 g          867 t
ORIGIN      
        1 cggtaagaga agttcatcat acttggcgca gttacgcgat gtttctgcta gaggtgatgc
       61 cgtaaagaac ggcagaacat taccggaaca agatagcggt ctacctgcac tggtatcaga
      121 cccggggctt ccccggatga tctccacggt actttaggta ctgtgtctat attatgtagg
      181 taggatcaat taactttgta gttgaaattc tttcattgaa ataatgaata aaaaagacaa
      241 accgcattaa acaatgccag aatataattg catttttgtt ccgttttttt aaatcatagt
      301 atacaaagaa gcttgcatac tatatgaaaa tataacaccc tttactatga tgtttataaa
      361 acaatatatg gagtcatgta ggtatgaatt tagatggtgt tagaccatac tgtagaacag
      421 tcaataaaaa gaatgaaagc atatcagata ttgcatttgc acatataata aaaagggtaa
      481 aaaattcatc atgtactcac ccaaaagcag cattggtttt tttaggagag aaaggttttt
      541 gtgatagcaa tgatgttcta tctattatgg gacaacaaat accaagagta tttaagaaca
      601 agatgttata tgattatgtt tttaaaaatg aaaaaagtaa aaatgatttt ctaaaaatgg
      661 ctgaatcatg gctaccacag agtgaaccaa tagtaataaa taatgatgat gacgcattga
      721 atgctgctgc ttatttttct gtaaaaaaag cgaaaataaa aacagtaaac gatactgatt
      781 ttaaagagta taataaggtt tatattcttg ggcacggtag tcctggttct catcaattag
      841 gccttggttc ggaacttatt gatgtacaaa caatcatttc aagaatgaaa gactgtggta
      901 ttctaaatgt gaaagatatc cgttttactt catgcggctc cgctgataaa gtggctccta
      961 aaaattttaa caatgcccct gctgaaagtc tttcttgtat ccttaactct ctgccttttt
     1021 ttaaggaaaa agaatctttg ctagagcaga taaaaaaaca ccttgaaaac gatgagtcat
     1081 tgagtgatgg tctaaaaata tccggctatc atggatatgg agttcactat ggtcaagagc
     1141 tttttcccta ctcacattat cgttcaactt caattcctgc tgatccggag catacagtaa
     1201 aaagaagctc tcagaaaaag acttttatta ttaataaaga actggattag tataaaattt
     1261 ttaacctata gttaattgtt tgcatttaag atgaagttat ttttttcttt agtagaaaag
     1321 tgcttcccta tgagggaaaa tcacaaatca tcataataca gagacaaaac gatacgaaca
     1381 aatagataaa aaacatcatt cttttactac attatttttc gtgaatatga aaaataatgt
     1441 tattacttta atataagact attttttgtt tttccatcac tctgttcaaa tttttccgca
     1501 tgacttgtgt tttttgtaac acagctcgtt ttttacagct gaccaaaatc atcaattaat
     1561 tatgctaagg aaataaatta tgaaaaccaa aaactttctt cttttttgta ttgctacaaa
     1621 tatgattttt atcccctcag caaatgctct gaaggcagaa ggttttctca ctcaacaaac
     1681 ttcaccagac agtttgtcaa tacttccgcc gcctccggca gaggattcag tagtatttca
     1741 ggctgacaaa gctcattatg aattcggccg ctcgctccgg gatgctaatc gtgtacgtct
     1801 cgctagcgaa gatgcatact acgagaattt tggtcttgca ttttcagatg cttatggcat
     1861 ggatatttca agggaaaata ccccaatctt atatcagttg ttaacacaag tactacagga
     1921 tagccatgat tacgccgtgc gtaacgccaa agaatattat aaaagagttc gtccattcgt
     1981 tatttataaa gacgcaacct gtacacctga taaagatgag aacatggcta tcactggctc
     2041 ttatccctct ggtcatgcat cctttggttg ggcagtagca ctgatacttg cggagattaa
     2101 tcctcaacgt aaagcggaaa tacttcgacg tggatatgag tttggagaaa gtcgggtcat
     2161 ctgcggtgcg cattggcaaa gcgatgtaga ggctgggcgt ttaatgggag catcggttgt
     2221 tgcagtactt cataatacac ctgaatttac caaaagcctt agcgaagcca aaaaagagtt
     2281 tgaagaatta aatactccta ccaatgaact gaccccataa agctggacag cctgtatcag
     2341 gctatggagg gcccatagac aaatctaccc tatatgagca taggaggagt ctatgggcac
     2401 accacgtttt acccctgaat ttaagggatt actggaaagg ctgggacata tccctccggc
     2461 agaagcagaa aaagcttatt atgctgccat cggaaacgat gatctggcaa cctgagttca
     2521 cagataaaac attctctagg aaactcgggg cggttccgtt caccacatgc aatgtggtgt
     2581 tgcaggggaa cggtctgcac catcccctat gtcgatcaat ataacagaaa tgacaacttc
     2641 agattcaggg cacaacctaa atatatttta ggtcacctct caaatcgttt gcctgatacc
     2701 gctccatttt ttaataaaaa aatcaatcat ttttgaatct ttgcttttca ttgcattgtc
     2761 aagcatagtg tccccactat ttgctttttg aaattttttg tttacatcaa ccaatccatt
     2821 attgataaaa tattctagca
//
LOCUS       DAJTMC010000277         2792 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.281, whole genome shotgun sequence.
ACCESSION   DAJTMC010000277 DAJTMC010000000
VERSION     DAJTMC010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2792
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.281"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(55..2707)
                     /locus_tag="OO130_004121"
                     /pseudo
     CDS             complement(55..2707)
                     /locus_tag="OO130_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415720.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT          653 a          699 c          675 g          765 t
ORIGIN      
        1 gctgcttaaa aaaagaacaa atggtagtgt ccgctattgc cagtacacct cattcacata
       61 gacattcagc tggctgttgc ctgcggtgat gtcatacccc agtatcatgc tggcacgccc
      121 cagcagcgat tcgtagtgat tcagatgtat attgaggcca ttactcgcct tcatagccat
      181 ctcattctgg tggctgtatg taagctgggt ttgcggctct atatagaacc cataaccagt
      241 aggggacagg ttgaacctct gcccggcttc tagggagatg ctcattccat tcgcagtgcc
      301 gttggcgtta acgccgttgt tatgactgtc cagtacgtgg aaactatttt tctggcgcga
      361 tgcttttata acgagatcgc tgtaaaaacc gttttgtgcc atgtaactgg cgtacattcc
      421 catgtagtct gaacgtgcgg taccgtcgcc tccgctatag tccggcgatg catgtgttga
      481 gccaatatac agaccgacat acaacggcat ttcatcagag agacgtttat ccccaccaaa
      541 ctggataccg ctgtaaccca tgtcaaagcc gctcagtttg ccactggcaa aggagtccag
      601 gcttccccca taactgcgca accagatatt accgtcttta ctctgatttc gcaggtcacc
      661 catccgttgc atcagcgtac ggttttcaac atagttcaat aagtaaccga cattgagata
      721 attgccgcca gcatctgcgg tcgtggtggg tttaggcgtg ggagcgggtt caggcgtagg
      781 atcggggttg actatgggag gctgagcggg agctggtgtg ggttcaggat taggagtcgg
      841 ttccggaact gtcccggaag cgtaaagctc ccagttagtg ccatttttac gcacatcgta
      901 cagatatccc ccccaactca acctgagaag acgcgctgaa cgaggccgcg ccgtcagtgg
      961 tttttaccac tgtcagaact tcatttcccg ttgtggcctc gctgccctgg ttgcggatag
     1021 ccaatacgtg attaccagca ctactcccgc tgatattcaa taaatcccct ttattattaa
     1081 cgccattacc ctcgccaaca acatcagcac gcataataaa ggtactgtta ccgctcaggt
     1141 tctctacgtt taatgtggca aatgtgccgg cagttgaccc gtggctggca aaatcgacag
     1201 ttgaatggct cagcgccagc gtgtcgagat tagaattact tgttacgttc cagacgctgt
     1261 tattcattgc aacgtccagt tttccgccat tgacattatc gctgagggag gaacctgtcc
     1321 aaaccgaccc agggtgcata tccagattga ttagcccacc tttggaaaaa atgctaccgt
     1381 tgataagcat acgacccgag gcgttgatgc ggctggcggc ataaccatcg tcatgttgcg
     1441 ttgcaatggc catcttgtcg ggtgtactca tatcaatgac cagatcgctc gtgaggtcga
     1501 tctggctgtt ggtcatggca taaattcccc tggctcctgc cgcgccggtg atagccaaac
     1561 tgtctccggt tattctaccg ccgctgagcg cccacaaacc cagcgccaga ctgccattac
     1621 gatcaactgt aatatcggta ttttgcatgt tgacaacagc cgttgccgtc tgggccgagg
     1681 caccataaga accgccggaa aagatgctgt ttcgttgcgc tgccgtgcca gaaaaattga
     1741 ttgtcgcgtc tgaaccactg gtgacgaggc caccgccctg cgcggaagaa atatggctat
     1801 ctgcaccgat agtggttgta ccaccacgaa cttcgacgcc attggccgcg gctccagtta
     1861 catcaacagt gagtgcattc gcgctaacct ggccaaagct ccagaggccg tgtgcattat
     1921 cgccattggt tttaatggaa ctgtttgttc cgagatcgac aacagagttt ttctgtacgt
     1981 ttatccccat ggcgctgtag ccctgaacat cgattgtcag gtctgtagcc gtaaaacgcg
     2041 cagcaccatt ggcgttattg ccgttgagac caccgatata aacacctgta cttccatcgg
     2101 tcgtgatttt acttccgctt ccaagatcga cactggtgcc atagtcattg atggttaggc
     2161 ctataccgtt cgagttttca atggtgaatt gagtggctgt tagtgttgag ctatgcccaa
     2221 taatgatgcc gtcatcgtta ctcttaatgg tgctgcctgt gcctaagtca gcatgggtat
     2281 agtcaccaat taagttaatg ccgatggcag aggtttgacc aacaacatct actgtgagtc
     2341 ggttagctgt cagggagctg tttttgccct gaataatgat tccttttgca tatccagagg
     2401 cgtcgttgac attgacggtg acatcattac ccaggttgat attgccaggc gtgtttcctg
     2461 ctggggtcat tacaccatag aggtttcctg atggatcggc ggtatctcct gtaattctgt
     2521 caccatcatt aatagtaata ttggtgccat attgacttcc tgtatcagca gctatcagca
     2581 ggggggaaaa gataattgca ttcaaaccaa tagcgccaaa ttttatcagt tgaattgagg
     2641 gcgaacggat ttttaattca gcgagacgat ctgctttggt attcccattg tgttgtttga
     2701 tgcccatacc actcctatat agtacccaga ctatgggtct atggattttg tcattttgtt
     2761 taaatcaatg aataatatcc tcttatcatc at
//
LOCUS       DAJTMC010000278         2740 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.282, whole genome shotgun sequence.
ACCESSION   DAJTMC010000278 DAJTMC010000000
VERSION     DAJTMC010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2740
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.282"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            <1..172
                     /locus_tag="OO130_004122"
     CDS             <1..172
                     /locus_tag="OO130_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="acts in anaerobic beta-oxidation of fatty acids;
                     possible ligase/synthase involved in anaerobic growth on
                     fatty acids; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cyclohexanecarboxylate-CoA ligase"
                     /protein_id="HCR5367300.1"
                     /translation="EVVAFFSRKRVAKYKYPEHIVVIEKLPRTASGKIQKFLLRKDIM
                     RRLTQDVCEEIE"
     gene            complement(229..2607)
                     /gene="ppsA"
                     /locus_tag="OO130_004123"
     CDS             complement(229..2607)
                     /gene="ppsA"
                     /locus_tag="OO130_004123"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HCR5367301.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNGRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
BASE COUNT          653 a          761 c          683 g          643 t
ORIGIN      
        1 agaggtggtg gcttttttta gccgtaaacg ggtcgcaaaa tataaatatc ctgaacatat
       61 cgtggtaatc gaaaaactac cgcgcactgc ctcaggtaaa atacaaaagt ttttgttaag
      121 aaaagatatt atgcggcgat taacgcagga tgtctgtgaa gagattgaat aagtttcatc
      181 ttcggggatc acataaaccc ggcgacaaaa cgccgccggg tatttatttt atttcttcag
      241 ttcagccagg cttaaccagg tttgcaccac ggtgtccgga ttcagagaca ggctatcgat
      301 cccctcttcc atcaaccatg cggcaaaatc ttcgtggtcg gacggaccct gaccgcaaat
      361 cccgacatat ttgccctgtt tcttcgcggc acggatagcc atcgacagca atgctttcac
      421 cgcatcgttg cgctcatcga acagttcaga caccacgccg gagtcacgat ccagaccgag
      481 cgccaactgc gtcatgtcgt ttgagccaat tgagaagccg tcgaaatatt cgaggaactg
      541 ctcggccagc aaggcgttgg acgggatttc acacatcatg atgattttca gcccgttctc
      601 accacgcttc agcccctgac gcgccagttc ttcaaccacc gctttcgcct ggtctacggt
      661 acgcacgaac gggatcatga tctcaacgtt ggtcagcccc atgtcgttgc gcacacgttt
      721 cactgcttca cactccagcg cgaagcagtc gcggaagctg tcggaaacat agcggcccgc
      781 gccacggaag ccgagcatcg ggttctcttc atctggctcg taacgctcac caccgaccag
      841 gttggcatat tcgttcgatt taaaatcaga gagacggaca atgacgcgct tcggataaaa
      901 cgcggcaccc agcgtcgcga tcccttcagt cagacgacca acgtaaaatt cacgcggaga
      961 atcaaaacct ttcatcatct cgcggatttc gttttgcaac tgcggttcct gatcgtcaaa
     1021 ctcaagcagt gcgcgtgggt ggacgccaat catacggttg atgataaatt ccagacgcgc
     1081 aagtcccacg ccttcgttcg gcagacaggc gaagtcgaaa gctcgatccg ggttaccgac
     1141 gttcatcatc actttcaacg gcagatccgg catggtttct acgctggagc ttttcacgct
     1201 aaattccagc aactccgcat agacgtaacc ggtatcacct tcggcacaag aaacagtgac
     1261 gttctcaccg tctttcatcc gttctgttgc atcaccacag cccacgaccg ccggaatacc
     1321 cagttcacga gcgatgatcg ccgcgtgaca ggtacgaccg ccacggttgg tgactatggc
     1381 agatgctttc ttcatgatcg gttcccagtc cgggtcggtc atgtcagtga ccagcacgtc
     1441 accaggttcg atgcggttca tttcgctgat gtcatggatg actttcaccg gacccgcacc
     1501 gatgcgatga ccgatagcac ggccttcggc gataatctta ccctgtgaat gcagcgtata
     1561 acgctccatg acctgaccgc gtgagcgcac ggtttccgga cgcgcctgca caatgaacag
     1621 tttgccggtg tggccatctt tcgcccactc aatatccatc gggcgaccgt agtgtttctc
     1681 aatttgtacg gcctgttttg ccagttcctg cacttcttcg ttggtcagcg agaagatgtc
     1741 acgctgttcc tgcggtacgt cttcgatttt aacctgcttg ccgtgctcct gggtcggcgc
     1801 gtaaaccatg cggatttttt tcgaccccat ggtgcggcgc acgatagccg ggcgattcgc
     1861 cgccagtgtc ggtttatgca cgtaaaactc atccgggtta accgccccct gcacgaccat
     1921 ctcaccaagg ccccatgcgg aagtgataaa caccacctgg tcaaagccgg attcggtatc
     1981 aatggagaac atcacgccag aagatgcgag gtcggagcgc accatccgct gaacaccggc
     2041 ggagagcgcc acgccacggt gatcgtaacc ctggtgcaca cgataagaga tggcgcgacc
     2101 gttaaacaga gaagcaaata catgtttcac tgccacgaga acggcgtcaa acccctgaac
     2161 gttgaggaag gtttcctgct gaccggcaaa ggaagcgtcc ggcatatctt ctgcggtggc
     2221 ggaggagcgc accgcaaaag aggcgttttc gtcatcggcg gaaagctgag cataggcttc
     2281 gcggatggcg ttttccagct caggctggaa gggagtgtcg ataatccact ggcggatttg
     2341 cgcgcccgct ttcgcaagct gagtaacatc gtcaatatcc gttttatcca gcagttcata
     2401 aatgcgctgg tttacgccgc tttggtccag aaactggtta aacgcgtcgg cggttgtggc
     2461 gaaaccattc ggaacggaaa cgcccattcc ggaaaggtta gtaatcattt cacccaggga
     2521 ggcatttttg cccccaaccc tgtctacatc attcatgccg agttggttgt accaaagcac
     2581 cagcggtgac gagccattgt tggacatcga acaatccttt tgtgataaat gaacggtttg
     2641 agaaacacat ttctgcgcat ttatctttgc atatttaacc ggatgaaaaa aacggtgaat
     2701 cgttcaagca aatatatttt tttacttttt aagactgatc
//
LOCUS       DAJTMC010000279         2716 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.283, whole genome shotgun sequence.
ACCESSION   DAJTMC010000279 DAJTMC010000000
VERSION     DAJTMC010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2716
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.283"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(<1..197)
                     /locus_tag="OO130_004124"
     CDS             complement(<1..197)
                     /locus_tag="OO130_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="mutations in this gene result in a compromised
                     ability for drug-inducible mexXY expression; expression is
                     inducible by the same ribosome-targeting agents that
                     induce mexXY; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HCR5367302.1"
                     /translation="MGNYIRPLSDAVFTIASDDQWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSVGR"
     gene            complement(443..895)
                     /locus_tag="OO130_004125"
     CDS             complement(443..895)
                     /locus_tag="OO130_004125"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5367303.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPLIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGEWFINFYMRYKQQH"
     gene            complement(892..1944)
                     /gene="dinB"
                     /locus_tag="OO130_004126"
     CDS             complement(892..1944)
                     /gene="dinB"
                     /locus_tag="OO130_004126"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HCR5367304.1"
                     /translation="MKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTAN
                     YPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSLD
                     EAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNGQ
                     FVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVMLLKRFGKFG
                     RILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAKV
                     KPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGLH
                     VTLLDPQMERQLVLGL"
     gene            complement(2003..>2716)
                     /locus_tag="OO130_004127"
     CDS             complement(2003..>2716)
                     /locus_tag="OO130_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000640586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5367305.1"
                     /translation="RDNVDAFLTLSATENMEFDSPMQVTTQNMQSGQTVTDNVQRAPR
                     TITISGVVVVGESGSILISRDTKLVENFIDTLENWRDQKQIISVICKDGIKIDDSIIT
                     SFKASKDVGISNGLRIQLTFQEINFKAIVGQTDISAATGKTATTNDGGATSKKNTGNT
                     TTSLGSPMLTCKELFSYSASELSDEALKARVTCSKSVSVKNGESTFTASANEQASKVL
                     KSGNALRKYSVNPNKKGTY"
BASE COUNT          707 a          660 c          617 g          732 t
ORIGIN      
        1 aaacgaccaa cagagaagaa cgctgcgcca atcggatagc cgcgtccggg atgtaaatct
       61 ggcatcccca ctacgcgctg catgttgggt aaatttgccg tggtgtgtaa ttgttggatc
      121 gctaaactct cgatccactg gtcatcagat gcgatggtaa ataccgcatc ggataaggga
      181 cgaatataat tgcccatata ccattccgta aatagactcg tcatacttca agttgcatgt
      241 gttgcgactt gaattattga gagtaacaat taaaaaatca ggaaatggca gacgaatcca
      301 gggcaagaaa aaaataatta atcctggaaa ggtctgcaaa gagtcattga taaaggattt
      361 gtcatgattt acctccttgc agtttgtgga ataagaaaac gcgcacatac taggcttgcg
      421 ttattttcat tgcaagctgg atttaatgtt gctgtttata acgcatataa aaattaataa
      481 accactctcc ccgacattca acgcgttgct gcttaactgt ctgaaaacca taacgttcaa
      541 aaaagggttt tgcggttatg cttgcgtcca ccgtaagttc ggattcagac ttaatcaaag
      601 gttttaacaa agcgctggca accccacggc gggtgtattc agggtcaaca aataacatat
      661 cgatatagtg ttcaacgcag gtaataaagc cgaccagttt tgcattaatg actgcaaccc
      721 gcacttgtga tttcgcgagt ttctccttcc agcgagattc gtcaatctgc gcccaggcgg
      781 caatttgttg tggtgaataa tgctgactgg cagtcatcgt aaccgctcta atgaaaatag
      841 cgcatagttg ctgaaaatcg ccaggctgat agtctcttat ttgtatgtta ttcataatcc
      901 cagcacgagt tgtctttcca tttgcgggtc aagcaacgtc acatgcagcc ccaccagtcg
      961 cacaccgcgc ccgccgcggc gttcatccca ggttttacgc gcggtggcga ttaagtcagc
     1021 tttattcagc cgcggccaga cgtgctcctg agtggtttgc tgaaaatcat caaactttaa
     1081 tttcaccccc tggcgagcaa tcagtaaatc aggtttcacc tttgccagac ggcgttcaag
     1141 ttccggatac agccgctcga taatcgcttc acattcagac cagtggtgga tatcttccgc
     1201 catcgtgcgt tccacgccga cggattttcg caaccgttcg ctgttaacgt cgcgctcgtc
     1261 aatcccctga ctacgctccc acaaaatgcg gccaaatttg ccaaagcgtt taagcagcat
     1321 caccagatca cacttttgta catcaccgca ggttcgtagc cccatcgctt ccagttttgc
     1381 cgccgagact ttgccgacgc cggggatttt tgccagtggt aaggtttgta aaaatgccgg
     1441 aacttctgcc ggcgtaatca caaactggcc gttgggttta ttcatgtcgg aggcgatttt
     1501 ggcgagaaac tttacgggtg ccacgcccgc agacgccgtc agttgcagct cgttgaaaat
     1561 cgtctggcgg atttcctggg cgatgagggt cgcagaaccg tggcaatgga cgctatcggt
     1621 gacgtcgaga taagcctcat ccagtgacaa cggttcaata cgcgaggtgt agcgcgagaa
     1681 gatttcgcgg atatgatttg aggcttcttt gtaggcgtca aagcgccccg gaagcaaggt
     1741 gagatgcggg cataatttga gcgccatccc tgtcggcata gcgctacgta cgccaaattt
     1801 acgcgcggga taattggcgg tactgatcac cccccgacgt tcgcggctgc cgccaatagc
     1861 aatagggata tcgcgcaggg cgggattgtc gcgcatctcc accgccgcga aaaagcagtc
     1921 catatccaca tgaataatct tcactttctt atacaccctg tatatttata catgcaaaga
     1981 gattatacac acaaaagcaa aatcaataag tgcccttctt attcggattc acagagtact
     2041 tccgtaaagc attacccgac ttaagcacct ttgaagcttg ctcattagct gaggctgtga
     2101 aggtgctctc accattttta acgctaacgc tcttagagca cgttacacgg gcttttagcg
     2161 cttcatctga taactcactt gccgaatagc taaaaagctc tttacaggtc agcatagggc
     2221 ttccaaggct cgttgtagtg ttgccagtat tctttttact ggtagcacca ccatcattcg
     2281 tggtagcagt cttgccagtt gcagccgaaa tatcagtctg acccacaata gctttgaagt
     2341 taatttcctg aaaagttagc tggattctta gaccatttga aataccaaca tctttagagg
     2401 ctttaaagct cgtgataatg gaatcatcaa ttttaattcc gtctttacag atgaccgaaa
     2461 taatctgctt ctggtcacgc cagttttcca gagtgtcgat gaagttctcc accagcttag
     2521 tatctcgtga gataaggata ctaccggatt caccaactac aacgacacca ctaatagtga
     2581 ttgttctggg tgctctttgc acattatctg tgacggtttg ccctgattgc atgttctgtg
     2641 tagtcacctg catagggctg tcaaattcca tgttttcagt tgctgataag gttaagaagg
     2701 catctacatt atctct
//
LOCUS       DAJTMC010000280         2679 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.284, whole genome shotgun sequence.
ACCESSION   DAJTMC010000280 DAJTMC010000000
VERSION     DAJTMC010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2679
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.284"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            231..770
                     /locus_tag="OO130_004128"
     CDS             231..770
                     /locus_tag="OO130_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015870862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HCR5367306.1"
                     /translation="MSHGGWKEISHPQTLFTRQKGDGWTVVPKNLRIDFYTKDNDFTK
                     GLSVLSEVNKRHTEAQKGLTPSLNISKDDLKVLANARNIPQSKLEEEMMSQAIYRKEY
                     ATSNEKIKNYALYYHEQAQNIIQRHQDGLGNKNIDIAFITDKNHKKHLSDIFKVINES
                     GVKYDVIHFGACRVNREEK"
     gene            complement(996..1319)
                     /locus_tag="OO130_004129"
     CDS             complement(996..1319)
                     /locus_tag="OO130_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nikA protein"
                     /protein_id="HCR5367307.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLR
                     AAALGKNVNSLTDDRVLKEVMRLGALQKKLFIDGKRIGDREYAEVLIAITEYHRALLS
                     RLMAD"
     gene            1603..1797
                     /locus_tag="OO130_004130"
     CDS             1603..1797
                     /locus_tag="OO130_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HCR5367308.1"
                     /translation="MNKQQQAVLNMAGFIKSQSLTLLEKLDALDADEQATMCEKLHEL
                     AEELQNSIQTRFEVENSTER"
BASE COUNT          867 a          657 c          568 g          587 t
ORIGIN      
        1 aaaaaaaaac aataaaaaac accgcaaaaa tggattgtta acaaatgaaa atactcaatt
       61 cgaatctcat aacaaacata tcatcaacaa taagaaatca ccttctaaca tcatccaaaa
      121 atataaaaac acacccatca ccaaacatgg acgtaacaag tcatcgtcgt ttattccaga
      181 aagacggaat aggattgagc caaacaggaa aagcaacaca acttgtcatt atgtcgcatg
      241 gtggatggaa agagatctcc catccacaaa cactattcac gcgtcaaaaa ggcgatggat
      301 ggactgtagt ccccaaaaac ctaagaattg atttttatac aaaagacaac gacttcacaa
      361 agggactaag cgttctttca gaggtaaaca aacgccatac tgaagctcaa aaagggttaa
      421 caccatcact aaacataagc aaagatgacc taaaagttct agcgaacgcg cgcaatatac
      481 ctcaatcaaa attagaagaa gaaatgatgt cacaagccat atatagaaaa gaatacgcaa
      541 caagtaatga gaagataaaa aactatgctc tatattacca tgaacaagcc caaaatatta
      601 tacaaagaca tcaagatggc ttaggaaaca aaaatattga tatagcattt ataactgata
      661 aaaatcataa gaagcacctt tctgacatat ttaaagtaat aaatgaatca ggagtaaaat
      721 atgatgtaat tcactttggc gcttgccgag taaatagaga ggagaaataa tcatcattta
      781 ccataatccc ttaattgtac gcaccactga aacgcgctga acgcgattct gacgcagaca
      841 ccacaaaaat agatgtttta ccccggaaaa acaggaagga tgcgtcagga tcgttttcag
      901 ctcgctgcat agctatgcat gaaagcgaat ggagatcact ttgggagctt accgtgttca
      961 taccgtctgt tttcaacagt tttctccccg ggaagctaat ctgccataag cctggataac
     1021 agggcacggt gatactccgt aatagcgatc agcacctccg catactccct gtccccgata
     1081 cgcttgccgt cgataaagag ttttttctgt aacgccccca gtctcataac ttctttcagt
     1141 acccgatcat cagtcagtga gttaacgttc ttaccgagag ctgccgcccg taaaaaaccg
     1201 gaaacggtct tgccgcattc agcagccctt tttctgattt cctggtcttc ttctgccgtc
     1261 agacgaacag ggcgactgat agcccgcctg cgcgtattac ttccacttcg ttttgtcata
     1321 aaacatggct ccgtatctga catgggtgtc ggggcaaagc cctgaccagg gcaattgtaa
     1381 tagcgtgcat gtatgcgcgg tataacaatt gcacatcctg tcctgtcagc aagctcgaat
     1441 taccgacaaa ggcacggcag ttttttggca gttacagtgt tgtctgacag aaatgcccgt
     1501 caaaaagcca ggcggcagaa tagcggcagc aaaacagtcg aaaagtggta cgcgatataa
     1561 tcacataacg cagatgacag acagaacgga gaaaacgaaa agatgaacaa gcagcagcaa
     1621 gccgtactca acatggcggg attcataaaa agccagagcc tgacgctgct cgaaaaactg
     1681 gacgcactcg atgctgacga gcaggccacc atgtgtgaga aactacacga actcgcagaa
     1741 gaactccaga acagcataca gacacgcttt gaagtagaaa atagcacaga gagataacgc
     1801 tcaggacagg atgtcgaacg gcacaacaga gcgtaaggag aaaatgtcac ataaggcgct
     1861 cttccgcttc ctcgctcact gactcgctac gctcggtcgt tcgactgcgg cgagcggtat
     1921 cagctcactc aaaggcggta atgcggttat ccacagaatc aggggataac gccggaaaga
     1981 acatgtgagc aaaaagccga aggcagggac aaaggccgcg caggtggcgt ttttccatag
     2041 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     2101 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     2161 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     2221 ttctcatagc tcacgctgtt ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     2281 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     2341 tgagtccaac ccggtaagac acgaattatc gccactggca gcagccactg ataaccgaat
     2401 tagaggagta agacttgaag tcatgcgcct gttaaggcta aactgaaaga acaagttttg
     2461 gtgactgcgc tcctccaagc cagttacctc ggctcaaaga gttggtatct ctgagaacct
     2521 tcgaaaaacc gccttgcagg gcggtttttt cgtttacagg cagtagatta cgcgcagaaa
     2581 aaaaggatct caagaagaac ctttaatttt ttttcacact tggtatccta ctttaaccca
     2641 taaaataaat acttacaccg acacgccctt aaacaaaag
//
LOCUS       DAJTMC010000281         2645 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.285, whole genome shotgun sequence.
ACCESSION   DAJTMC010000281 DAJTMC010000000
VERSION     DAJTMC010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2645
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.285"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            21..857
                     /locus_tag="OO130_004131"
     CDS             21..857
                     /locus_tag="OO130_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5367309.1"
                     /translation="MQKNGDTLSGGLTFENDSILAWIRNTDWAKIGFKNDADGDTDSY
                     MWFETGDNGNEYFKWRSRQSTTTKDLMTLKWDALNILVNAVINGSLGVGSTNALGGSS
                     IVLGDNDTGFKQNGDGILDVYANSQRVFRFQNGVAIAFKNIQAGDGKKFTLSSSNNST
                     KNATFNLWGASTRPVVAELGNEAGWHFYSQRNTDNSVIFSVNGQIQPSNWGNFDSRYV
                     KDVRLGTRVVQLMARGGRYEKAGHAITGLRIIGEVDGDDEAIFRPIQKYINGTWYNVA
                     QV"
     gene            826..1389
                     /locus_tag="OO130_004132"
     CDS             826..1389
                     /locus_tag="OO130_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000930007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR5367310.1"
                     /translation="MAHGITSHRCKLCSILKNIKSGNPKTKEQYQLTKNFDVIWLWSE
                     DGKNWYEEVNNFQDDTIKIVYDENNIIVAITKDAPTLNPEGFSVVEIPDITANRRADD
                     SGKWMFKDGTVVKRIYTADEQQQQAESQKVALLSEAESVIQPLERAVRLNMAMDEERT
                     RLESWERYSVLVSRVDTANPEWPQKPE"
     gene            complement(1418..1951)
                     /locus_tag="OO130_004133"
     CDS             complement(1418..1951)
                     /locus_tag="OO130_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000972171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR5367311.1"
                     /translation="MMHLKNITAGNPKTKEQYQLTKQFNIKWLYSDDGKNWYEEQKNF
                     QPDTLKMVYYHNGVIICIEKDVSAINPEGASVVELPDITANRRADISGKWLFKDGVVI
                     KRTYTEEEQRQQAENEKQSLLQLVRDKTQLWDSQLRLGIISDENKQKLTEWMLYAQKV
                     ESTDTSSLPVTFPEQPE"
     gene            complement(1954..2511)
                     /locus_tag="OO130_004134"
     CDS             complement(1954..2511)
                     /locus_tag="OO130_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5367312.1"
                     /translation="MAGVGFDGSSDISISAKNVNAFALRQTGNTVNGDTSVGWNWDSG
                     AYNALIGGASALILHFNINAGSCPAVQFRVNYKNGGISYRSARDGYGVELGWSDFYTT
                     TRKPSAGDVGAYTRAECNSRFITGIRLGGLSSVQTWNGPGWSDRSGYVVTGSVNGNRD
                     ELIDTTQARPIQYCINGTWYNAGSI"
BASE COUNT          755 a          567 c          586 g          737 t
ORIGIN      
        1 taaacagggc tggtaacgcc gtgcaaaaaa atggcgatac cttgtccggt gggcttactt
       61 ttgaaaatga ctcaatcctt gcctggattc gaaatactga ctgggcgaag attggattta
      121 aaaatgatgc cgatggtgac actgattcat acatgtggtt tgaaacaggc gacaacggca
      181 atgaatattt caaatggaga agccgccaga gcaccacaac aaaagacctg atgacgctga
      241 aatgggatgc actaaatatt cttgttaatg ctgtcattaa tggcagtctt ggagttggtt
      301 cgacgaatgc gttaggtggt agctctattg ttcttggtga taatgacacc ggatttaaac
      361 agaatggaga cggtattctg gatgtttatg ctaatagtca gcgtgtattc cgctttcaga
      421 atggagtggc tattgctttt aaaaatattc aggccgggga tggtaaaaaa ttcacgctat
      481 ccagctccaa caactccaca aagaatgcaa cgtttaattt atggggtgct tcaacccgtc
      541 cagtagttgc agagttaggc aatgaggcag gatggcattt ctatagccag cgaaatacag
      601 ataactcggt aatattttct gttaacggtc agatacagcc cagcaactgg gggaattttg
      661 attcacgcta tgtaaaagat gttcgcctgg gtacgcgtgt tgttcaattg atggcgcgtg
      721 gtggtcgtta tgaaaaagcc ggacacgcaa ttaccggatt aagaatcatt ggtgaagtag
      781 atggcgatga tgaagccatc ttcagaccaa tacaaaaata catcaatggc acatggtata
      841 acgtcgcaca ggtgtaaatt atgcagcata ttaaaaaata ttaagtccgg aaatccaaaa
      901 acaaaagaac aatatcagct aacaaagaat tttgatgtta tctggttatg gtccgaagac
      961 ggtaaaaact ggtatgagga agtaaataac tttcaggacg acaccataaa gattgtatac
     1021 gacgaaaata atattattgt tgccataacc aaagatgccc caacgcttaa tcccgaaggc
     1081 tttagtgtcg ttgagattcc agatataaca gccaatcgtc gtgccgatga ttcagggaag
     1141 tggatgttta aggacggaac tgtggttaag cggatttata cagcagacga acagcaacaa
     1201 caggccgaat cacaaaaggt cgcgttgctt tctgaagctg aaagcgttat tcagccactg
     1261 gaacgcgctg tcaggctgaa tatggcgatg gatgaggaac gcacacgact ggagtcatgg
     1321 gaacgctaca gcgttctggt cagccgtgtg gatacagcaa atcccgaatg gccacaaaag
     1381 cctgagtaaa aattaaggcc caatatcggg ccttctctca ttctggttgt tcgggaaacg
     1441 ttactggcag gctggaggtg tctgtagatt cgactttctg cgcatagagc atccactcgg
     1501 ttaatttttg tttattctcg tcggaaatga tgcccagccg tagctgtgag tcccatagct
     1561 gggttttatc cctgacaagt tgcaacaggc tttgcttttc attttccgct tgttgcctct
     1621 gctcttcctc ggtataagtt cgctttatca ctacgccatc tttgaacaac catttccccg
     1681 aaatatcagc ccggcgattt gctgtaatat caggtaattc aacgacgctt gcgccttccg
     1741 gattaattgc tgaaacatcc ttttcaatac aaataataac gccgttatgg taatagacca
     1801 ttttcaaagt gtctggctgg aaattctttt gttcctcata ccagtttttc ccatcatctg
     1861 aataaagcca tttgatgtta aattgctttg ttagctggta ttgctctttt gttttagggt
     1921 tgccagcagt aatgtttttt aagtgcatca tcgttaaata ctccccgcgt tataccacgt
     1981 cccattaatg caatactgaa ttggccttgc ctgtgttgta tcaattaatt catcacggtt
     2041 tccgttaact gaacccgtaa cgacataacc tgacctgtca gaccagccgg gaccattcca
     2101 tgtctgaaca gatgacagac cgccaaggcg aatacctgta ataaaccttg agttacattc
     2161 tgcccgcgta tatgcaccaa catctcccgc tgaaggtttt cgtgtcgtgg tatagaaatc
     2221 tgaccaacct aattcaaccc cataaccatc acgagccgac ctgtaggaaa tgccaccatt
     2281 tttataattc acacggaatt gtacggcagg acagctacca gcatttatat taaagtgaag
     2341 aattaatgca gatgcaccac caatcagggc gttatatgca ccactatccc aattccatcc
     2401 aacggatgta tcaccattaa cagtattacc tgtttgtcgg agtgcaaatg cattgacatt
     2461 tttggcacta attgaaatat cgctagaacc atcaaaacca actccagcaa tttttcgtgc
     2521 tgttttcagc tttgttgcag tagcggcatt gccggacagt tccccagaaa ggccaccgct
     2581 gaatgtttgt cgattggtcc aggtattcgc tgtactgagt aacggtattt tctccccgct
     2641 tgtgc
//
LOCUS       DAJTMC010000282         2564 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.286, whole genome shotgun sequence.
ACCESSION   DAJTMC010000282 DAJTMC010000000
VERSION     DAJTMC010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2564
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.286"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(<1..213)
                     /locus_tag="OO130_004135"
     CDS             complement(<1..213)
                     /locus_tag="OO130_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5367313.1"
                     /translation="MSRKTQRYSTEFKAEAVKTVPENQLSISEGASRLSVPEGTLGQW
                     VTAARKGLNTPGSRTVAELESEVMQLR"
     gene            762..905
                     /locus_tag="OO130_004136"
                     /pseudo
     CDS             762..905
                     /locus_tag="OO130_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558263.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            1052..1320
                     /locus_tag="OO130_004137"
                     /pseudo
     CDS             1052..1320
                     /locus_tag="OO130_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001449236.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1716..>2564
                     /locus_tag="OO130_004138"
     CDS             1716..>2564
                     /locus_tag="OO130_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR5367314.1"
                     /translation="MIKSTNIQAIGSGIMHQINNIYSLTPFPLPMELTPSCNEFYLKA
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQEAELNLSELDLKTLPDLPPQITTLEIRKNL
                     LTHLPDLPPMLKVIHAQFNQLESLPALPETLEELNAGDNKIKELPFLPENLTHLRVHN
                     NRLHILPLLPPELKLLVVSGNRLDSIPPFPDKLEGLAMANNFIEQLPELPFSMNRAVL
                     MNNNLTTLPESVLRLAQNAFVNVAGNPLSGHTMRTLQQITTGPDYSGPRIFFSMGNSA
                     TISAPEH"
BASE COUNT          824 a          558 c          454 g          728 t
ORIGIN      
        1 acgcagttgc ataacttcag attccagctc agccactgtg cgggagcctg gtgtattgag
       61 cccttttctt gcagcggtaa cccattgtcc taaagtgcct tcaggaacag ataatcggga
      121 agcgccttca ctgatcgaaa gctgattttc aggaaccgtt ttgacggctt cggctttaaa
      181 ctccgtagag taacgttggg tttttctgct cattattggc tccttctgat gccattctat
      241 tccaggaagg agtgtccgtt aaactcaggc tacctcaact ctcgtctggg gaatatctcc
      301 ccggcagcct tcaggataaa atattatcag atgactgctt agaaaaagaa caaatggtgg
      361 tgtccgctat tgccagtaca cctcaagctt cttaccatat ttgaaataaa gctcaatagc
      421 gcaaattttt tgttcttcag taaacatgtt tcatcttctg agagtccagg aaatcgtccg
      481 catccccatt accgtcttat atcactggcg ctgacatgtg ctcattgatg aagtgaatct
      541 gaatgacatg ttttgccata tcaacgccaa ccggtgtata tttcatttgt ggagtctcca
      601 gtctgaggac gctttatgca tcccgtattg ggcagtatga tgccggaaag ctgcgaggct
      661 ccacacctgc cagtttcacc cttttaacca tcatactact ccgtgtttaa ttgtgtggga
      721 tgcgttcatt acattttaac agtgtcaccc cgtcggccac gaaagataaa caggtgacca
      781 gaaaagggat cttcatccag cacatgctat atcttgaacg taacaacctg accctccgca
      841 cccgcatcaa gcggctggca cgtaaaacaa tctgcttctc gcgttccgtg gaaatccacg
      901 aaaagtcatc ggctccttca ttgaaaaaca catattctac tgattggaag cgccacccga
      961 aaaagtatcg tttttttaca gtaaacttca tttagccgac tataatgtaa aaaaataaat
     1021 tttttaccga aatataacat aaggaagtaa tatgcttaca caaactatat tcccttgtct
     1081 tcccaaaaaa caagaaaata tcatccctga agtatcaaat actatattag tatcttctac
     1141 tgttactaca gatggatgta ctgtttttaa taaacaaaaa cagccatata cgaattgcaa
     1201 atacctgcta ccaaccgaac gaaaaagttg aaattcacag ctacagaaat acaatggttg
     1261 acaaagataa atgaagccgg aattgatgaa aaacaaagcc aacggtattc tgatttctga
     1321 agttaatttc agactcgttt aagctccatt ttcaaaaaaa aaaatatatc agggtttgtt
     1381 actaataact tctgaaaaca gaaaaaaaaa tagagcagta attctatttc agagaggagt
     1441 aactccatct atcctcgata cgtactgaac gttatcgtga tatattcatc atgctttctt
     1501 cagcattatt cgagtacagg gagagacgca atgaccttaa ataccttttt atggctgaat
     1561 taccattctc aggaataaca gcagactcct tcccatagac ggcttagggg gaaagcaata
     1621 cccggagaaa gggtacaaag caaactgttt taaaattaat aagctatatt ctatttcact
     1681 agaattctta tcgctgataa gaaattttag ttataatgat taaatcaacc aatatacagg
     1741 caatcggttc tggtattatg catcaaataa acaatatata ctcgttaact ccatttcctt
     1801 tacctatgga actgactcca tcttgtaatg aattttattt aaaagcctgg agtgaatggg
     1861 aaaagaacgg taccccaggc gagcaacgca atatcgcctt caataggctg aaaatatgtt
     1921 tacaaaatca agaggcagaa ttaaatttat ctgagttaga tttaaaaaca ttaccagatt
     1981 taccgcctca gataacaaca ctggaaataa gaaaaaacct attaacacat ctccctgatt
     2041 taccaccaat gcttaaggta atacatgctc aatttaatca actggaaagc ttacctgcct
     2101 tacccgagac gttagaagag cttaatgcgg gtgataacaa gataaaagaa ttaccatttc
     2161 ttcctgaaaa tctaactcat ttacgggttc ataataaccg attgcatatt ctgccactat
     2221 tgccaccgga actaaaatta ctggtagttt ctggaaacag attagacagc attcccccct
     2281 ttccagataa gcttgaaggg ctggctatgg ctaataattt tatagaacaa ctaccggaat
     2341 taccttttag tatgaacagg gctgtgctaa tgaataataa tctgacaaca cttccggaaa
     2401 gtgtcctgag attagctcag aatgccttcg taaatgttgc aggtaatcca ctgtctggcc
     2461 ataccatgcg tacactacaa caaataacca ccggaccaga ttattctggt cctcgaatat
     2521 ttttctctat gggaaattct gccacaattt ccgctccaga acac
//
LOCUS       DAJTMC010000283         2549 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.287, whole genome shotgun sequence.
ACCESSION   DAJTMC010000283 DAJTMC010000000
VERSION     DAJTMC010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2549
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.287"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(1..191)
                     /locus_tag="OO130_004139"
                     /pseudo
     CDS             complement(1..191)
                     /locus_tag="OO130_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706790.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 family protein"
     gene            complement(191..790)
                     /locus_tag="OO130_004140"
     CDS             complement(191..790)
                     /locus_tag="OO130_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HCR5367315.1"
                     /translation="MAHIQLIKQTSSGLLLPATPESCDFLHQIKTGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFDYWTPIGGAITPRERELVSGFVEYLCESVGREHTPALSEAAEQ
                     YLNTVATRRTRDTALLKSFEAFREWVTIQAGFYTEHIYPDGSRGRRAKSISFANMDET
                     EFQQVYKSVLNVLWNWILFRKFSSPEQVENVAAQLLEFA"
     gene            complement(829..1116)
                     /locus_tag="OO130_004141"
     CDS             complement(829..1116)
                     /locus_tag="OO130_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5367316.1"
                     /translation="MKIEELREIFSENGLYAVRVENGAIVSHCRIRCLQSQQRKSGAV
                     LFYFCNGLLTDGFILREDEFVTSLRVLKEIGFKAGFLLLLKNKLIYNLEQD"
     gene            complement(1366..1521)
                     /gene="hokD"
                     /locus_tag="OO130_004142"
     CDS             complement(1366..1521)
                     /gene="hokD"
                     /locus_tag="OO130_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HCR5367317.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     VDYESRK"
     gene            complement(1791..2456)
                     /locus_tag="OO130_004143"
     CDS             complement(1791..2456)
                     /locus_tag="OO130_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HCR5367318.1"
                     /translation="MTVADFKRPKLELPNGANKLLLHSCCAPCSGEVMEALQASGIDY
                     TIFFYNPNIHPQKEYLIRKDENIRFAEQHGVPFIDADYDTDNWFERAKGMEWEPERGI
                     RCTMCFDMRFERTALYAAENGFSVISSSLGISRWKNMQQVNDCGRRAVAHYPGMVYWD
                     YNWRKQGGSSRMIEISKREKFYQQEYCGCVYSLRDTNLHRKSQGRPLIKIGQLHYGKE
                     EKE"
BASE COUNT          641 a          666 c          578 g          664 t
ORIGIN      
        1 ttctccagat ttcttgcgtg cgaaaaacac cttctgcatg catcaggcgt aattcttctt
       61 tggtgtaatc gcttgttttt acccgccagt cggtaatgcg ccagcacaga cgtttccgga
      121 tcgtgattgc agtagccagg aattctgacg gtgcacatct gcccccgcgc cgctttacgt
      181 aaatccacca ttacgcaaac tccagtagct gcgcggccac attttcgact tgttccggag
      241 aggaaaattt acggaacagg atccagttcc acagtacatt cagcacagat ttataaacct
      301 gctgaaactc ggtttcgtcc atattcgcaa acgagatgga tttcgccctg cgcccacggc
      361 taccgtccgg ataaatatgc tcggtgtaaa atccggcctg aatggttacc cactcgcgga
      421 aagcctcaaa cgactttagc aatgctgtat ctcgggttct gcgtgtcgca actgtattca
      481 gatattgctc tgcggcttcg ctcagagctg gcgtatgttc ccggcctact gattcgcaca
      541 ggtactcaac gaagcctgat accagttctc gttcgcgagg cgtaatcgcc ccaccgatcg
      601 gagtccagta atcgaatccc agttgcagga gtttgaaaaa acgcttgtgg aatgcgtagt
      661 tacgcacacg cttaaagtct gcgtgtatcc actcacctgt tttgatttga tgcagaaaat
      721 cgcaactctc cggcgtcgcc gggagaagta atccggaaga ggtttgtttg atcagttgta
      781 tatgcgccat cggctttctc cggtggcacg gtgttacaca gcaggagttc aatcctgctc
      841 aagattgtag atgagtttat tcttcagcaa aagcagaaaa ccagccttaa aaccaatctc
      901 tttcaaaacc cgtaatgatg tgacaaattc gtcctcacgc aaaataaaac cgtccgtcag
      961 aagtccatta caaaaataaa ataacacagc accgctcttc ctttgttgag attgcaaaca
     1021 tctaatgcgg caatggctga caatcgctcc attctcaacg cgcacagcat agaggccatt
     1081 ttcactaaaa atttcacgca attcttcgat tttcatcttc agaatccttc cagataaata
     1141 gctctcccct gttcggggtc catccctctt ctccctgcgc gctacttaag tgagtcgatt
     1201 ctatctgcga aggtgcgcga atcaaattca ccggaaataa acaacaaaaa acccgccgaa
     1261 acgctgagag atcacagcga tgggcgggtt aagtgcgggt gcgttgagga tgcctgacac
     1321 atcagaggtg ggcggggatg ggatcagctc cccgcccggt cactcttact tcctggattc
     1381 gtagtctacg aagacagcga cctccgtctg gccggttcgg attcgtacct cacagaggtc
     1441 tttcctcgtt accagtgccg tcactatgac ggttaaacag atgacgatca gggcgattaa
     1501 catcgccttt tgctgcttca tagcctgctt ctccttgcct ttcggcatgt aagaggctaa
     1561 cctacatgtg caaagcatga aattggcctc agattaatgt taagcgtctt gccggacgcg
     1621 taatgttaac tggggctttt ctctatctgc cttttggtgt tcatgcctga agcagatagc
     1681 ctgaagcaga tagcctgaag cagatagcct gaagcagata gcctcaagca cccgcagtca
     1741 ttctacttac ctcgccaata tgaaatcaat cagaaaggtg ccccataaaa tcactccttc
     1801 tcttctttac cgtagtggag ttgaccaatt ttgataagag ggcgtccctg agatttgcgg
     1861 tgtagattgg tatcgcgcag agaatacaca cagccacaat attcctgctg atagaatttt
     1921 tcgcgcttgc tgatttcaat catacgggac gagcctccct gcttgcgcca gttataatcc
     1981 cagtacacca tgcccggata atgcgcgacg gctcgccgcc cacagtcgtt aacctgctgc
     2041 atatttttcc agcgtgaaat gcccagtgaa ctgctgatca cactgaaacc attttcagcg
     2101 gcgtacagcg ctgtccgctc aaaacgcatg tcaaaacaca tggtgcaacg gatccctcgt
     2161 tcgggctccc attccattcc tttggctcgt tcaaaccagt tgtctgtgtc gtaatcagca
     2221 tcaataaacg gcacgccgtg ttgttcagca aagcgaatat tctcatcctt acgaattaaa
     2281 tactctttct gaggatgaat gttcgggttg tagaaaaaga tggtgtagtc gattcccgag
     2341 gcctgaagcg cctccatcac ctcaccggaa catggagcac agcaagagtg cagtagtagt
     2401 ttgtttgccc cgtttgggag ctccaatttg ggccgtttga aatcagcaac tgtcataaat
     2461 atgtttattg gggtcataaa aatatcaaag agtgtagcat tagaacgggg ctatcggaaa
     2521 cagatgtgtg actgctcccc gccctttcg
//
LOCUS       DAJTMC010000284         2511 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.288, whole genome shotgun sequence.
ACCESSION   DAJTMC010000284 DAJTMC010000000
VERSION     DAJTMC010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.288"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            93..545
                     /gene="yeeE"
                     /locus_tag="OO130_004144"
                     /pseudo
     CDS             93..545
                     /gene="yeeE"
                     /locus_tag="OO130_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416517.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transport protein YeeE"
     gene            559..786
                     /locus_tag="OO130_004145"
     CDS             559..786
                     /locus_tag="OO130_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HCR5367319.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(829..2253)
                     /gene="sbcB"
                     /locus_tag="OO130_004146"
     CDS             complement(829..2253)
                     /gene="sbcB"
                     /locus_tag="OO130_004146"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HCR5367320.1"
                     /translation="MTDTDKQPTFLFHDYETFGTHPALDRPAQFAAIRTDSEFNVIGE
                     PEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGYN
                     NVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDGL
                     PSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMA
                     LIDVPQMPPLVHVSGMFGAWSGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDSD
                     TLRERLYTAKTDLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNLK
                     ILRENPQVREKVVAIFAEAEPFTPTDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPA
                     LDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQMLV
                     QQYADDKEKVALLKALWQYAEEIV"
BASE COUNT          606 a          594 c          652 g          659 t
ORIGIN      
        1 gctgggttat cgctcgccac gggaatatct gcggcagcgg gcttgtaatg ggttaagtga
       61 taacagatgt ctggaaatat aggggcaaat ccactgctgt actcatcggt ttgattgcgc
      121 ttttagcctg gcccctgagt gaagcaaccg gacgcatgtt tgggttagga atcacttccc
      181 caacggccaa tattctgcaa tttctggtcg cgggtgacgt gaaatacatt aactggggcg
      241 ttttcctggt gttagggatc ttcgtggggt catttattgc agccaaagcg agccgtgagt
      301 tccgcgttcg cgcagctgat gcacaaacaa cattacgtag cgggttaggt ggtgtactga
      361 tgggcttcgg tgccagtatt gcaggtggtt gctctatcgg taatggactg gttatgactg
      421 caatgatgac ctggcagggc tggattggcc ttgtatttat gattctcgga gtctggactg
      481 catcctggct tgtgtatgtt cgaccgcagc gtaaggcgcg actggcgact gctgcggcaa
      541 attaagtaag gtgtaaagat ggcgattaaa aagctggatg tggtgacgca ggtctgtccg
      601 tttccactca tagaagcaaa agcagcactg gcagaaatgg ccagcggcga tgagttggta
      661 attgagtttg attgcaccca ggcaactgaa gcaatcccac agtgggcggc agaagaagga
      721 catgccatca ccgattatca gcagattggc gatgcagcgt ggagcatcac cgttcaaaaa
      781 gcctgactca acattgtcct ccgccgtacc agcggcggag gcttcaaatt agacgatctc
      841 ttccgcgtac tgccaaagtg cttttaacag cgccactttt tctttgtcat cggcatattg
      901 ttgtaccagc atctgcaatt catcagcata accctgcaaa aactctggcg tgaatacctg
      961 gcggcggtgc tccagccagc gttgctgctc ggcataatcc agcgtccccg ggaagttgcg
     1021 tgcccgataa ttgaacaaca gcttttcaat ccgtttatca acaaaagtga tatccagtgc
     1081 cggtaaatta cgtggctcgg tttccagcac aattttcatt gctgcacgat ctgcgtcact
     1141 gaaaaagccg ttataaagct gtgcatccac gttatctgta ggcgtaaacg gttcggcttc
     1201 cgcgaatatc gccaccactt tttcacgcac ttgcggattt tcacgcagaa ttttcaggtt
     1261 atcgaggcaa tgctgacgat taattcccag tcggtcggca tcttccgggc gtagcgtatt
     1321 cgcctgggcc agcaccggac atttattgat gtgcaccagc ttaaccggaa cggcggcgtt
     1381 atcgccaaga tcggtttttg cggtatataa acgctcgcgc aatgtgtcgc tatccagttc
     1441 cagtaatggc gaaatgtctc ctgccaaatc caccataatt acagcattgc gattttcagg
     1501 atgccacgcc agcggtgcca cccagctggt attgccgctc catgccccaa acattccgga
     1561 aacgtgaacc agggggggca tctgcggaac atcaatcaac gccatcagtt tgtgtttatt
     1621 acgatgggta aagagataat caaacaggcg tggctgacgc gtttttacca gtttcgccat
     1681 cgcaatagtg gcgtacacat cagccatcgc atcgtgggcg ttgctatgtt caataccatt
     1741 cgctttggtt aaatgctcaa ggcgaaagct cggtagaccg tcatcatttt caggccagtt
     1801 tattccttcc gggcgcaggg cataacaggc acgcataaca tccaacaaat cccagcgcga
     1861 gttatcatgc tgccagctcc aggcgtaagg atcgtagaaa ttacgataaa aaacgttgcg
     1921 tgtgacttcg tcgtcgaaac gcacattgtt gtagcccaga atacaggtct tcggtacggt
     1981 aaaaagcgag tgaatacggg cggcaaacgc ggcttcgttt tctcctttcg cccgtgcttc
     2041 ctgcggggta ataccggtaa ttaatactgc tccaggctgg ggtaaatagt catccgcggg
     2101 cttgcagtaa aagacttcgg gttcgccgat gacattgaat tcgctatcgg tgcgaatggc
     2161 tgcgaactgt gcagggcgat ctaacgcggg gtgcgtgcca aaggtttcgt aatcgtgaaa
     2221 gaggaaggta ggttgtttat ctgtgtctgt cattgtctag cattgtccac tgtagttaat
     2281 taactgtctg ttataactcg aaaaacaacc attttttcac ctgagtatgt ctacgaacgt
     2341 caaatatagt tgtttactgt gcgctgttgt ccatgtgata ttgtgtacca gatgtgtacc
     2401 aaaccattat ttctgagcgt accaaatatt atttatggct ataagcgaca caaaactgcg
     2461 tagtatttat ggtaaaccat attcgggccc acaagaagtg gctgatgccg a
//
LOCUS       DAJTMC010000285         2509 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.289, whole genome shotgun sequence.
ACCESSION   DAJTMC010000285 DAJTMC010000000
VERSION     DAJTMC010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2509
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.289"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(162..581)
                     /locus_tag="OO130_004147"
     CDS             complement(162..581)
                     /locus_tag="OO130_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5367321.1"
                     /translation="MVHEDKARKEFASRLALACENAGYEQHGRQAEIARRMKLTPKAV
                     SKWFNGETIPRREKLRELATLIGTTPTYLLGEDTEESGQVRFYQELNPRQKIIIDLLD
                     ELPDSETDELLKTLEEKKQKYNAIYEELARKKKQKAS"
     gene            661..915
                     /locus_tag="OO130_004148"
     CDS             661..915
                     /locus_tag="OO130_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR5367322.1"
                     /translation="MNQKTLEDVIKTVRVAVVADVCGVSQRAIYKWMDNGKLPRTEYT
                     GETNYAEKIALASNGLFSADAILTIGRNKTTTKKLMGVDS"
     gene            912..1337
                     /locus_tag="OO130_004149"
     CDS             912..1337
                     /locus_tag="OO130_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCR5367323.1"
                     /translation="MKIKHEHIRMAMNAWAHPDGEKVPAAKITKAYFELGMTFPELYD
                     DSHPEALARNTQKIFRWVEKDTPDALKKIQALLPAIEKAMPPLLVARMRSHSSAYFRE
                     LVETRERLVRDADDFVAVAIAGFNQMNRGGPAGNIVAVH"
     gene            1409..2479
                     /locus_tag="OO130_004150"
     CDS             1409..2479
                     /locus_tag="OO130_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer"
                     /protein_id="HCR5367324.1"
                     /translation="MANAWLRLWHDMPNDPKWRTIARVSGQPIATVMAVYIHLLVSAS
                     RNVTRGHIDVTTEDLASALDVTEEVIDSILQTMQGRVLDGDLITGWEKRQVLKEDNGN
                     ISQTAKSPAERKRAQRERERKREQNGDCHGASRYVTHMSRRVTTDKDTDKDTDQEDQN
                     TMVHGVKNATNQAGDVQTVNPGQPAGTTPEADSAYALKADSGAVQQAMTASPEQSHQL
                     QQPEADSAIQREADRVVPENTGQSVGRVDYPDVFEQVWREYPLRAGANPKKSAFSAWK
                     ARLREGVPPEAMLDGVRRYARYLAATGKTGTEFVQRATTFFGPDRNFENPWLLPVSGT
                     NNQRCVNHISEPDNEIPPGFRG"
BASE COUNT          674 a          548 c          668 g          619 t
ORIGIN      
        1 gtattgccct gcattctgtg gggcggggtg ggtggcaggt atataatgta ctttgcgttc
       61 attgttgtaa agtgcttatg gtacattttg tgtgtaaaaa aatgagatgg gataaagtga
      121 agcacaaacc cggaggaagg cgctaccgga tttatgctgg tttaagaggc tttttgtttt
      181 ttctttcgtg ctaactcttc gtaaattgca ttgtacttct gttttttctc ttcaagagtt
      241 tttaaaagtt catctgtctc actgtcaggg agctcgtcca gaaggtcaat gatgattttt
      301 tgtcttggat ttaactcctg atagaaacgt acctgtccac tttcttctgt atcctctccc
      361 aaaagatagg ttggtgttgt tcctattagt gttgctaatt cccttaattt ctcccggcga
      421 ggaattgttt cgccattaaa ccatttgcta accgcttttg gtgttaattt cattcgacgg
      481 gcaatttctg cctgccttcc atgttgttca taaccagcgt tttcacaggc tagcgcaagc
      541 ctactggcga actctttacg cgctttatct tcatgaacca taagttcaat gatattcgct
      601 cttgaatgta ctgtcagttc tgttatagca tgtactcaaa gttcacattg tgagggtgat
      661 atgaaccaga aaacacttga agatgtaatc aaaactgttc gcgttgctgt tgtggccgac
      721 gtttgtggtg tcagccaaag agcaatctat aaatggatgg ataacggaaa attgcctcgc
      781 acagaatata ccggcgaaac aaattacgct gaaaaaatcg ctcttgcatc aaacggatta
      841 ttttctgccg atgcaatttt aactattggc aggaataaaa ctactacgaa aaagctgatg
      901 ggagttgatt catgaaaatc aagcatgaac acatccgcat ggcgatgaat gcctgggcgc
      961 atccggacgg cgaaaaagta ccggctgcga aaattaccaa agcgtatttc gagctgggaa
     1021 tgacgttccc ggaactgtat gacgacagcc atccggaagc cctggctcgc aatactcaga
     1081 aaattttccg ctgggtggag aaagacaccc ctgatgcgct taaaaaaatt caggcgttgt
     1141 taccagctat cgaaaaagca atgccacctc tgctggtggc ccgaatgcgc agccatagct
     1201 cagcctattt tcgggaacta gtggagacgc gggaacgact ggtgagagac gctgatgatt
     1261 ttgtcgcagt ggcgatcgct ggtttcaacc agatgaatcg tggtggccct gcaggaaata
     1321 ttgtggctgt gcattgactc gcaatattca taccggatca cttccggcaa tttgtgagta
     1381 aaaagattcg gtatcaaaag aggtgagtat ggctaacgcc tggctcagat tatggcatga
     1441 catgccaaat gaccctaagt ggcgaacaat tgccagggtg tcagggcagc caattgcaac
     1501 agtgatggca gtgtatatcc acctcctggt gagcgcgtca cgaaatgtca cgcgaggtca
     1561 cattgatgtc acgacagaag atttggcaag tgcgctcgac gtgacagaag aggtaattga
     1621 ttcaattttg cagacgatgc aggggcgggt acttgatggt gatttaatca ctggatggga
     1681 aaaacgccag gtgctgaaag aggacaacgg caatatttcg caaaccgcaa aatctcctgc
     1741 agagcgcaag agggcgcagc gagagaggga aagaaagcgg gaacaaaatg gcgattgtca
     1801 cggcgcgtca cgatatgtca cgcacatgtc acgacgagtc acgacagata aagatacaga
     1861 taaagataca gatcaagaag atcaaaacac tatggtccat ggcgtaaaaa acgccacgaa
     1921 ccaggcaggg gatgttcaga ccgtcaatcc tggtcagcca gcaggcacga caccggaagc
     1981 cgattcagcg tatgcgctga aagccgattc gggcgctgtg cagcaggcga tgaccgcaag
     2041 tccggagcaa tcacaccaac tgcagcagcc tgaagccgat tccgccattc agcgggaagc
     2101 cgatcgggta gtcccggaaa acaccgggca gtctgtggga cgagtggatt atccggatgt
     2161 gttcgaacag gtctggcggg agtacccgtt gcgtgccggg gcaaacccga agaaatccgc
     2221 tttcagtgcc tggaaggcca gattacgcga gggggtgcca ccagaggcca tgctggatgg
     2281 cgtgaggcgt tacgcaagat acctggcggc taccgggaaa acgggaacgg aatttgttca
     2341 gcgagcgacg acgttttttg gaccggaccg gaattttgag aacccctggt tgctcccggt
     2401 aagcggcacg aacaaccagc gttgtgtgaa tcatatttct gaaccggata acgaaattcc
     2461 gccgggcttc agggggtaag tgttaatttc tggtcatgag gtaattttc
//
LOCUS       DAJTMC010000286         2494 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.290, whole genome shotgun sequence.
ACCESSION   DAJTMC010000286 DAJTMC010000000
VERSION     DAJTMC010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2494
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.290"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            <1..493
                     /locus_tag="OO130_004151"
     CDS             <1..493
                     /locus_tag="OO130_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HCR5367325.1"
                     /translation="TMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHA
                     MAYQLGGFYNLPHGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEA
                     CINAIRELAKKVDIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVAIYR
                     AAM"
     gene            658..2196
                     /gene="aldB"
                     /locus_tag="OO130_004152"
     CDS             658..2196
                     /gene="aldB"
                     /locus_tag="OO130_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HCR5367326.1"
                     /translation="MTNNPPSAQIKPGEYGFPLKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
BASE COUNT          598 a          622 c          707 g          567 t
ORIGIN      
        1 gaccatgatt gccgaaaacc tgccgttagc cgttgaagat ggcagtaatg cgaaagcgcg
       61 tgaagcaatg gcttatgccc agttccttgc cggtatggcg ttcaataatg cttctctggg
      121 ttatgttcat gcgatggcgt accagctggg cggtttctac aacctgccac acggtgtatg
      181 taacgccgtt ctgctgcccc atgttcaggt attcaacagc aaagtcgccg ctgcacgtct
      241 gcgtgactgt gccgctgcaa tgggcgtgaa cgtgacaggt aaaaacgacg cggaaggtgc
      301 tgaagcctgc attaacgcca tccgtgaact ggcgaagaaa gtggatatcc cggcaggcct
      361 acgcgacctg aacgtgaaag aagaagattt cgcggttctg gcgactaatg ccctgaaaga
      421 tgcctgtggt tttactaacc cgatccaggc aactcacgaa gaaattgtgg cgatttatcg
      481 cgcagcgatg taatcatcat ttccacaacg gctggcaaat tgtcagccgc tttttcaact
      541 atctctgtaa cccttgcccg taaattcgtg atagccgtcg taaagctgtt accgactggc
      601 gaagatttcg ccagtcacgt ctacccttgt tatacctcac accgcaagga gacgatcatg
      661 accaataatc ccccttcagc acagattaag cccggcgagt atggtttccc cctcaagtta
      721 aaagcccgct atgacaactt tattggcggc gaatgggtag cccctgccga tggtgagtat
      781 taccagaacc tgacgccggt gactgggcag ctgctgtgcg aagtggcgtc ttcgggcaaa
      841 cgagacatcg atctggcgct agatgctgcg cacaaagtga aagataaatg ggcgcacacc
      901 tcggtgcagg atcgcgcggc gattctgttt aagattgccg atcgaatgga acaaaacctc
      961 gagctgttag cgacagctga aacctgggat aacggcaaac ccattcgcga aaccagtgct
     1021 gccgacgtgc cgctggcgat tgatcatttt cgctatttcg cctcgtgtat tcgggcgcag
     1081 gaaggtggga tcagtgaagt tgatagcgaa accgtggcct atcatttcca tgaaccgtta
     1141 ggcgtggtgg ggcagattat cccgtggaac ttcccgctgc tgatggcgag ctggaaaatg
     1201 gctcccgcgc tggcggcggg caactgtgtg gtgctgaaac ccgcacgtct taccccgctt
     1261 tctgtactgc tgctaatgga aattgtcggt gatttactgc cgccgggcgt ggtgaacgtg
     1321 gtcaatggcg caggtgggga aattggcgaa tatctggcga cctcgaaacg catcgccaaa
     1381 gtagcgttta ccggctcaac ggaagtgggc caacaaatta tgcaatacgc aacgcaaaac
     1441 attattccgg tgacgctgga gttgggcggt aagtcgccaa atatcttctt tgctgatgtg
     1501 atggatgaag aagatgcctt tttcgataaa gcgctggaag gctttgcact gtttgccttt
     1561 aaccagggcg aagtttgcac ctgtccgagt cgtgctttag tgcaggaatc tatctacgaa
     1621 cgctttatgg aacgcgccat ccgccgtgtc gaaagcattc gtagcggtaa cccgctcgac
     1681 agcgtgacgc aaatgggcgc gcaggtttct cacgggcaac tggaaaccat cctcaactac
     1741 attgatatcg gtaaaaaaga gggcgctgac gtgctcacag gcgggcggcg caagctgctg
     1801 gaaggtgaac tgaaagacgg ctactacctc gaaccgacga ttctgtttgg tcagaacaat
     1861 atgcgggtgt tccaggagga gatttttggc ccggtgctgg cggtgaccac cttcaaaacg
     1921 atggaagaag cgctggagct ggcgaacgat acgcaatatg gcctgggcgc gggcgtctgg
     1981 agccgcaacg gtaatctggc ctataagatg gggcgcggca tacaggctgg gcgcgtgtgg
     2041 accaactgtt atcacgctta cccggcacat gcggcgtttg gtggctacaa acaatcaggt
     2101 atcggtcgcg aaacccacaa gatgatgctg gagcattacc agcaaaccaa gtgcctgctg
     2161 gtgagctact cggataaacc gttggggctg ttctgatata agaagctggt cgcattgggt
     2221 attcattgcc tgatgtgacg cttacgcgtc ttatcatgcc tacgggaacc tgaccgtagg
     2281 ccggataagg cgtacacgcc gcatccggca taaacaaagc gcacgttgtt acaatttacg
     2341 ttttaaacct acgtgcgcaa taatccagac tacgccaaca gaagtgcgtt tctggggcgt
     2401 gttttgtcag gagtttaatc tcgcgtttcg atggtgagcg ggcgaactag gccgtaaatc
     2461 accattggtg atccgaggcc aatcagcaat aaac
//
LOCUS       DAJTMC010000287         2470 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.291, whole genome shotgun sequence.
ACCESSION   DAJTMC010000287 DAJTMC010000000
VERSION     DAJTMC010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2470
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.291"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            128..1264
                     /gene="potA"
                     /locus_tag="OO130_004153"
     CDS             128..1264
                     /gene="potA"
                     /locus_tag="OO130_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HCR5367327.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMREGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            1248..2111
                     /gene="potB"
                     /locus_tag="OO130_004154"
     CDS             1248..2111
                     /gene="potB"
                     /locus_tag="OO130_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HCR5367328.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVSELDD"
     gene            complement(2343..2414)
                     /locus_tag="OO130_004155"
                     /pseudo
     CDS             complement(2343..2414)
                     /locus_tag="OO130_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387760.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
BASE COUNT          594 a          606 c          622 g          648 t
ORIGIN      
        1 ctaagccagt aattcacaac aaagattggc gattcaagcg gtttgccgta gaattaccgc
       61 cctcattaag agtcaccaag gtggttaacc acaaaccccg catcggtaag ccatccgttg
      121 cgtttacatg ggacagagta aaaaattgaa taaacaaccg agttcgcttt caccgctggt
      181 gcaattggcg ggaattcgca aatgctttga tggtaaagag gtcattcccc agctggatct
      241 gactatcaac aatggcgagt tcctcacgct gcttggccct tctggctgcg gtaaaacaac
      301 cgttcttcgc ctgattgcag gtctggaaac tgttgattcc ggacgcatca tgctggataa
      361 cgaggacatc acccacgttc cggcggaaaa ccgctatgtg aacactgttt tccaaagcta
      421 cgcacttttc ccccacatga ccgtgttcga aaatgtggcc tttgggttgc gcatgcaaaa
      481 aacccccgct gctgaaatta cgccccgcgt gatggaagcc ctgcggatgg tgcaactgga
      541 aaccttcgct caacgtaaac cgcatcagct ctctggtggt caacagcaac gcgtcgccat
      601 tgctcgcgcg gtggttaaca agcctcgtct gttgttgctg gatgagtcgc tctcagcgct
      661 ggattacaaa ctgcgtaagc aaatgcagaa cgagctgaaa gcgttacagc gtaagcttgg
      721 cattacattc gtcttcgtga ctcacgacca ggaagaagca ctcaccatgt cagacaggat
      781 tgtggtgatg cgcgagggtc gcattgagca agacggcacg ccgcgtgaaa tctacgaaga
      841 gccgaaaaac ctgtttgttg ccggcttcat tggcgaaatt aatatgttta acgccacggt
      901 gatcgagcga ctcgacgagc agcgcgtacg cgccaacgtt gaaggccgcg aatgtaatat
      961 ctacgttaac ttcgccgttg aaccggggca aaaactgcat gttctgctgc gcccggaaga
     1021 cttacgtgtt gaagagatta acgacgacaa ccacgctgaa gggctgattg gttacgttcg
     1081 tgagcgtaac tacaaaggca tgacgctgga gtcggttgtt gaactggaaa atggcaagat
     1141 ggtgatggtc agcgaattct tcaatgaaga cgatcctgac tttgaccact ctctcgacca
     1201 aaaaatggcc attaattggg tagaaagctg ggaggtcgta ctggctgatg aagaacacaa
     1261 gtaagttcca gaatgtagtg attgtcacta ttgtcggttg gcttgtgttg tttgtctttc
     1321 tgcccaacct gatgatcatt ggcaccagct ttttgacccg cgacgacgcc agtttcgtca
     1381 aaatggtctt tacgctggat aactacacgc gtctgctcga tccgctctat tttgaagtgc
     1441 tattgcactc gctgaatatg gcgctgatcg ccaccctcgc ctgcctggtg ctgggctatc
     1501 catttgcctg gtttctggcg aagctgccac acaaggtgcg tccgctgctg ctgtttctgc
     1561 tgattgttcc gttctggacc aactcattga ttcgtatcta cgggctgaaa attttcctca
     1621 gcaccaaagg ctatctcaac gagtttttgc tctggctggg cgttatcgac acaccaatcc
     1681 gtatcatgtt cacgcccagt gcggtgatta tcggtctggt ttacattctg ctgccgttta
     1741 tggtgatgcc gctgtactcc agtatcgaaa aactggataa gccgttgctt gaggcggcgc
     1801 gcgatctcgg tgccagcaag ttacagactt ttatccgcat cattattcca ctgacgatgc
     1861 cggggattat tgccggatgt ctgctggtga tgctgccagc gatgggcctg ttctatgtgt
     1921 ccgacctgat gggcggtgcg aaaaacctgc tgatcggtaa cgtcatcaag gtacagttcc
     1981 tcaatattcg tgactggccg tttggtgcag ccaccagcat tacgctgact atcgtaatgg
     2041 gcctgatgtt gctggtttac tggcgcgctt ctcgtttgct gaataagaag gtgagcgaat
     2101 tagatgatta atcatttata ttcaatgcat taagtttgtt tttgagtggt tttgccgttt
     2161 aaggtaacgg cgttatagat cctatcaaaa tcaatcaatt ccattctggt tttggagaaa
     2221 gatttttcga gagcttcggt ctttaactaa aactgattgc gtataacccc aatacccctc
     2281 cccgactatc tgaatggtcg gggaggcact tcacgcacaa aatatcatcc ctgcgccgtt
     2341 gattactctg caggtgaagt cggccactca atatcaggtg cagttgatgt atcaacacgg
     2401 ttcagcaaca cctgaagtgg cacactgaat ttggccacct gaacagaggt gatatgctca
     2461 cctcagaaca
//
LOCUS       DAJTMC010000288         2448 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.292, whole genome shotgun sequence.
ACCESSION   DAJTMC010000288 DAJTMC010000000
VERSION     DAJTMC010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2448
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.292"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(<1..753)
                     /locus_tag="OO130_004156"
     CDS             complement(<1..753)
                     /locus_tag="OO130_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCR5367329.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESMPVFHNTRVLVEPEPKSMRN
                     LPSGVVPAVRQPLVEDKTLLPFFSNARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIGHAISGTQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPGEQTATSILKQR
                     TKNLALLPHAHQQQNPPQEETVVSIAVDPESPA"
     gene            complement(755..1033)
                     /locus_tag="OO130_004157"
     CDS             complement(755..1033)
                     /locus_tag="OO130_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5367330.1"
                     /translation="MAHDTKFYNSDNSAAPASRHGRRSHAFKSDWYQHDPCTEEQAEW
                     LIQRYRRHGYEIKKALSLDYRHWIIFVRLPYSERPPRPSCTFQQRIWR"
     gene            974..1159
                     /locus_tag="OO130_004158"
     CDS             974..1159
                     /locus_tag="OO130_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5367331.1"
                     /translation="MPAGRRGRVIRIVKFGIVRHLFSLLAQQVPVVQADGWIVNQNDQ
                     KKQNPPKRVKCGCVEDA"
     gene            complement(1201..1356)
                     /gene="hokD"
                     /locus_tag="OO130_004159"
     CDS             complement(1201..1356)
                     /gene="hokD"
                     /locus_tag="OO130_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HCR5367332.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            complement(2008..2424)
                     /locus_tag="OO130_004160"
     CDS             complement(2008..2424)
                     /locus_tag="OO130_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5367333.1"
                     /translation="MTELTKEQLIEEAKLKIAITKCHPNSGMARVEGELFKIARASLE
                     AEPIAWRYRYVKKGVMDSQGELWVGDWKYVPKKEDCNDRPNYEIQALFTAPPVPVTSE
                     ELVKAVHFYEQLKRENPPASGNQINGLTMSVKRPAN"
BASE COUNT          579 a          640 c          575 g          654 t
ORIGIN      
        1 agccggagat tccggatcaa cggcaatgct gaccaccgtc tcttcctgtg gtggattctg
       61 ttgctggtgg gcgtgaggca gcagcgcaag attttttgtg cgctgcttca gtatgctggt
      121 ggcggtctgc tctcccggca cgatgtcgct ttcacggtac attgagcgga ttttttccgc
      181 acgcaacccc agcgaacgac gtaatactgc ttccggtagc gcgtccgcca cctgattgcg
      241 gaccgcccac caggataatt cagccagaga taattcacgc tcctgcgtac cgcttattgc
      301 gtgaccgatg acgtcaatca tccatgctga caggttttga tgagcaagtt gctcgagtga
      361 ttcggatgtc tggtcacgca actggttgtc gcagtgccag cacaacacca ttgcgccggt
      421 accataacgg tgaatgacgg tttcgctgtg atgataatcg ccgtgtggcc actggcagga
      481 tttaatatgg cgcaacagcc agtcagacaa tgcaccagca ccaccagcag cacgaatcac
      541 ccgtgcgtta ctgaaaaacg gcagcaatgt tttgtcttcc actagcggct ggcgaacggc
      601 aggaacgacc ccggacggca gattacgcat gcttttcggt tccggctcca ccagtacccg
      661 ggtattgtgg aataccggca tggattcacg gcctggctta aggaccacca gcccgagttc
      721 cggaaccaga acaggtcgaa gtaatacccg cacattacct ccagatccgt tgctggaatg
      781 tgcaggacgg acgcggtggc cgttcggaat aagggagcct gacgaagatt atccagtgac
      841 gataatcgag actgagggct ttcttaatct cgtatccgtg tctgcggtag cgctgaatca
      901 gccattcagc ctgttcttcg gtgcaggggt cgtgctgata ccagtcagat ttgaatgcat
      961 gagaacgccg cccgtgcctg ctggcaggcg cggcagagtt atccgaattg taaaatttgg
     1021 tatcgtgcgc catctgtttt ctctgctggc gcagcaggtg ccagttgttc aggctgacgg
     1081 atggattgta aaccagaacg accagaaaaa acaaaacccg ccgaagcggg ttaagtgcgg
     1141 gtgcgttgag gatgcctgac acatcagcgg tggcgaggga tttctccccc gccgggtctc
     1201 ttactcctca ggttcgtaag ctgtgaagac agcgacctcc gtctggccgg ttcggattcg
     1261 tacctcgcag aggtctttcc tcgttaccag tgccgtcact atgacggtta aacagatgac
     1321 gataagggcg attaacatcg ccttttgctg cttcatagcc tgcttctcct tgcctttcgg
     1381 cacgtaagag gctaacctac atgtgttcag catggattga gcctcagatt aatgttaagc
     1441 gtcttgcagg acgcgtagtg ttaactgggg cttttctcta tctgcctttt ggtgttcatg
     1501 cctgagacag atagcctcaa gcacccgcag tcattctact taactaagat ttccctgcaa
     1561 accgtttttg tccggcacag taaatatcca actaaaccaa tagcgttcgc tgtatttacc
     1621 gccagtattc aatgcacatg accgccatga acacccctaa aaaaagggca tttatatgtc
     1681 caaacattaa tatcaaaaca tcaatttttt ccatatacct tgctgtgaag atgatgggca
     1741 tacatgatgc gaacaaccag aacgcaacaa acaaaaactg caatgcgttt ttcattattc
     1801 cccctacaat caatgtgcaa taacatttaa acacacctca atttggccgg acatataaat
     1861 atctaaacca gaaaaaatca cttacatagc gttacaaact ctttagtcta aatactcatc
     1921 gtaaaacatt ccccatactt atcagtccgt tccgcgccag gtagcttatt gccttatctg
     1981 gcaacctata atcaggtttc cgctttttca gttggctggt cgtttaaccg acatagttaa
     2041 cccattaatc tggttgccgg atgctggtgg attttcgcgt tttagttgtt cataaaagtg
     2101 cacagcttta accagttctt ctgatgtaac cgggactggt ggggcagtga ataaggcctg
     2161 aatttcatag ttcggcctgt cgttacaatc ctcttttttc ggtacatatt tccagtcacc
     2221 aacccacaac tccccctgag agtccataac accttttttc acgtagcgat atcgccacgc
     2281 tatcggctct gcttccagcg atgcccgtgc aattttgaat aactcgccct ctactcgcgc
     2341 catccctgaa ttggggtggc atttcgtaat cgctattttt aatttggctt cttcgattaa
     2401 ttgttctttt gttaattcag tcattttcat taccgccctt tcaggcgg
//
LOCUS       DAJTMC010000289         2440 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.293, whole genome shotgun sequence.
ACCESSION   DAJTMC010000289 DAJTMC010000000
VERSION     DAJTMC010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2440
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.293"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(80..871)
                     /gene="kdgR"
                     /locus_tag="OO130_004161"
     CDS             complement(80..871)
                     /gene="kdgR"
                     /locus_tag="OO130_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HCR5367334.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            1048..2421
                     /locus_tag="OO130_004162"
     CDS             1048..2421
                     /locus_tag="OO130_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5367335.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRSRA"
BASE COUNT          516 a          645 c          572 g          707 t
ORIGIN      
        1 taacgcacct tcaactttat ttttctcatg ctaaataaag aacctgtctg tttgatattc
       61 cgacaggttc ttactctcat cagaacggat agtcgtgata acccatttgg gcagaaattt
      121 tgcgcgctgc ggtatgcaac attgcgacat attcctgtaa acgctcttca gagaaacgca
      181 acgtcgggaa ggagatgctc aaaccggcaa tgaccacgcc aaagcgatca aataccggta
      241 ccgcaatgca tcgcagccct tcttcctgct cttcgttatc ttcgccatac ccctgctcgc
      301 gcacctggtc cagaacgggt aataacgctt ctgtactggt gatggtccgc tcggtactgc
      361 gtttatactc cacgccctca agaatttgct tcacttcatc gcgatcgcgc catgccagca
      421 gtaccttacc aatcgcggtg ctgtacagcg gattacgacg cccaatccgt gaatacatgc
      481 gcaaattgta catagagtca attttgtgaa tgtaaacaat actgtcttcg tccagtgcgc
      541 cgaggtggat agtttctttg gtcaggcggg agatctcacg catctggata tctgcgctac
      601 gaattaaatc gacgttttgt aacgcgcgag cgcccagttc aaacaatttc agggtcaggg
      661 aatatttctc cgactcccct tcctgcgcca cataacctaa ggttttcatg gtctgtaaaa
      721 agcgataaac ggtgcttttt gacatcatga cgcgctgcga cagctcggtt atccctattt
      781 cgcgctcttc acccagcgcc tgcaaaatgc caaaaacttt tagcacggaa gatacagaat
      841 caggctgttt atccagatct gcgttagcca tttatcacct cattgcgagt gttttataaa
      901 aatcagaact gttttttatt ataatttcgc accagggggg tcgcaatcca tcttttgccg
      961 gttagttaca attctgcgac atccaccgtg aatatcagtg ctagaatcat acccctgttg
     1021 attattcacc aaagatataa aattcctatg ccaaaagttc aggccgacgg cctgccattg
     1081 ccccagcgat acggtgcgat attaaccatt gtgattggta tttcgatggc tgtccttgac
     1141 ggcgcaatcg ccaacgtcgc cctgccaaca atcgccacgg accttcatgc cacgccagcc
     1201 agttccatct gggtagtgaa cgcctatcaa atcgccattg tcatctccct gctctcgttt
     1261 tcgtttctgg gcgatatgtt tggctatcga cgtatttata aatgcggtct ggtcgttttt
     1321 ctgttgtctt cactgttctg cgccctttct gattcgctgc aaatgctcac ccttgcgcgt
     1381 gtcatacaag gtttcggcgg tgcagcgttg atgagcgtta ataccgcact tatccgcctg
     1441 atctatccac aacgttttct ggggagaggg atgggcataa actcgtttat tgttgccgtc
     1501 tcttctgctg ccgggccgac aattgctgca gcaatcctct ccatcgcatc ctggaaatgg
     1561 ttatttttaa tcaacgtacc gttaggtatt atcgccctgc ttctggcgat gcgttttctg
     1621 ccacccaatg gttctcgcgc cagtaaaccc cgtttcgacc tgcccagcgc cgtgatgaac
     1681 gcgttaacct tcggcctgct tatcactgcg ttgagtggtt tcgctcaggg gcaatcgctg
     1741 acgttaattg ctgcggaact ggtggtaatg gttgttgttg gtattttctt tattcgccgc
     1801 cagctttctc ttcccgtacc gctgctaccg gtggatttac tgcgtatccc gctgttttca
     1861 ctttctattt gcacatctgt ttgctctttc tgcgcacaaa tgctggcaat ggtttccctg
     1921 cccttttacc tgcaaaccgt gctcgggcgt agtgaagtcg aaacaggttt acttctgaca
     1981 ccgtggccgt tagcaacgat ggtgatggct ccgttggcag gctatttgat tgaacgcgta
     2041 catgcaggat tgctgggtgc tctagggttg ttcatcatgg ctgcggggct tttttccctg
     2101 gttctgctgc ccgcgtcacc tgcggatatc aatattatct ggccgatgat cttatgtggc
     2161 gctggatttg gcttgttcca gtcacccaat aaccacacca ttattacctc cgctcctcgc
     2221 gaacgtagcg gtggagccag tggcatgtta ggaacggctc gtctactggg tcagagtagc
     2281 ggcgcggcac tggtggcgct gatgctaaat cagttcggtg ataatggtac gcacgtttcg
     2341 ctgatggctg cggctattct ggcagtgatt gctgcctgtg tcagtggttt acgtatcact
     2401 cagccacgat ccagggcata aaaaaagcgc gtcgatcagg
//
LOCUS       DAJTMC010000290         2380 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.295, whole genome shotgun sequence.
ACCESSION   DAJTMC010000290 DAJTMC010000000
VERSION     DAJTMC010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.295"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(<1..302)
                     /locus_tag="OO130_004163"
     CDS             complement(<1..302)
                     /locus_tag="OO130_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="catalyzes the transfer of alpha-xylosyl residue;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HCR5367336.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYA"
     gene            complement(312..1694)
                     /locus_tag="OO130_004164"
     CDS             complement(312..1694)
                     /locus_tag="OO130_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HCR5367337.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTVVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVLGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSATISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQGKRRCQ
                     QQFTSQEVQN"
     gene            1987..2081
                     /locus_tag="OO130_004165"
     tRNA            1987..2081
                     /locus_tag="OO130_004165"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2021..2023,aa:Sec,seq:tca)
     gene            2160..2324
                     /locus_tag="OO130_004166"
                     /pseudo
     CDS             2160..2324
                     /locus_tag="OO130_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280578.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="virulence RhuM family protein"
BASE COUNT          640 a          596 c          558 g          586 t
ORIGIN      
        1 gcataacgtt ctgtgttttc gattgtgacc ttcacgtcct gcaaaatatt gagcggataa
       61 tgaggaccgt tattcagcgc tccctgaaaa tgctcaatcc gcacaccgac aataccttcc
      121 tgtggggaga aaaagcgcag cgtaaataaa ggcgtatcaa gctgccaggt acgttcacgc
      181 acatcacggg gggcagcata gaccaccatt tcattatcct gctgttcaac ctcgaacacc
      241 tgaagcgggt gaatcaaatt gaggccaggt tgaatcaacc agtttccatc gctaattttc
      301 attgccgttc cttagttctg cacttcttga gaggtgaatt gttgctggca acggcgttta
      361 ccctgagcca gctgttccat aacggttttc agattgtgcg tcgtgagtga gtagtagcgt
      421 ttagcgataa tcgcgctcag caaataacag atcgccggaa caatcgtgaa tagcgcaata
      481 atgatgctaa tcgtggcgct gttctgcgct ttttctgccg catcatatcc gccataagcc
      541 agcatccagc cgataagagc gccgccgaag gccaacccca gtttgagcac aaacagcgtg
      601 ccagcaaaac tgatcccggt caggcgttta ccattgcacc actcgccgta gtcgacggta
      661 tcggacatca ttacccactg gataggtgtc accagttgat gcaacacacc aatcacgaag
      721 atgaagacaa acatagtgat gctggcctgc atgggaacaa agaacatcgc gaggctaatc
      781 actgccagca gggcgttcgt ccaccagaag atagtgactt tacatttcca gtcggtcaga
      841 ggttttgcca gtgcggaacc aatcaggtta cccacgcaat aagtggtgag aaaagcgaca
      901 aacacttccg gcgtgcccaa aatccatgtg acgtaataca tcatcgcccc accgagtacg
      961 cacaccgcca ggatattgaa aatggttagt aaaccgacaa tccgccactg gtcgttttgc
     1021 cagatatcac gtaaatcttc ccgcatagac gttgttgtag gtggtgcttc aacgcgttct
     1081 ttagtggtga agaaacaaaa tgccagcatc atgaatgcca ccacggaaag gaccgcgata
     1141 ccgccctgga aaccgagtgg tttattatca ccgccaatta aattaaccag tggcatcatc
     1201 agaacagtag aaagcatgcc tcccgcggtc gccagcacaa aacgccagga ttgcagcgag
     1261 atacgctgag tcgggtcatt ggtgattaca ccacccaatg cgcagtaagg gatattgacg
     1321 acggtataaa gtaaggtaag tagggtgtaa gtaattgctg catagatcat tttgccgttc
     1381 atactgagat ctggcgtgct ataggccagt acacagacga tcccgaatgg cagtgcgcca
     1441 aacagtaccc acggacgaaa tttaccccag cgagagcgcg ttcgatcggc caacaacccc
     1501 atgcaaggat cggaaatcgc atccagtgca cgagcgacca aaaacatggt tccgacaaaa
     1561 ccggcaggaa tgccaaaaat atcggtataa aagaacatca tatataacat tacgttatcg
     1621 aaaataatgt ggctggcggc gtctcccatg ccataaccaa ttttctcttt aacggacaac
     1681 acttcactct tcatctgctt ttccttgatg ctataggggt gctgagaccg ataacgcatt
     1741 ttgtaaacca tccctgtgag agcgaatatt ccgatatctg gttattaatt tatgattctt
     1801 gttttatgtg atcgtggtag cgttaattcc gctcatatgt cattgtaaaa tatgggtttt
     1861 atatgaacta taatgctttc gtgataatac gctgcgtgta ttaggcggaa aaaactgatc
     1921 tgggggatgt agaaactcaa ggaagtagct ataatgcgcc ccgcctccat gtagcaatcg
     1981 aggcgcggaa gatcgtcgtc tccggtgagg cggctggact tcaaatccag ttggggccgc
     2041 cagcggtccc ggacaggttc gactcctgtg atcttccgcc aaaatgcctc ttctgacgtt
     2101 ctccaacccc ttgtaatact gtcaatccag atattttcta ctgctcaata ttcaatcggc
     2161 ttcaggaata cctggtctaa ggttttgtga tggacgatga gcggctgaag aatccgcctg
     2221 tcggttcttc tgtcctcgaa cgcatccgcg atatgcgcgc cagcatgtgc cgtgtttatt
     2281 tgcaggtgat ttgccccctg ctggctctca gccaactgta tcgccattaa ggtgaagatg
     2341 ttgttttaca cgctccataa cacccttttt tatacgacta
//
LOCUS       DAJTMC010000291         2314 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.296, whole genome shotgun sequence.
ACCESSION   DAJTMC010000291 DAJTMC010000000
VERSION     DAJTMC010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2314
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.296"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(45..381)
                     /locus_tag="OO130_004167"
                     /pseudo
     CDS             complement(45..381)
                     /locus_tag="OO130_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(914..1240)
                     /locus_tag="OO130_004168"
     CDS             complement(914..1240)
                     /locus_tag="OO130_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5367338.1"
                     /translation="MFKYAEMLMAEEDNKVCELIFKQQDNLILKINDRFPRIIIKKYR
                     TLQGKVVWLWILPFVIEWLILCALGVNSWRGKSKYLDYYLLRDEMPVRLVSIIFPTLL
                     ALFLLA"
BASE COUNT          760 a          447 c          402 g          705 t
ORIGIN      
        1 aatatgaaaa gatggtttaa ataccccgtt acttttgact tacactatac ggtatcgcat
       61 cgtttaatat tcgcaccggc cagattttta tttctattag ttgtcacaat actgaatgcg
      121 tacgaccaca gtattctggc tcctgtgtgg tcatgcttta attctgcgtt ccggacagat
      181 aagcagttgc ttgcaggaat ccttcttgtg ttaatgtcag ttcccctttt taccagtgct
      241 gatttccaca ttccgtccaa cagagcttat agcctttccc tggattatag cattgtccgg
      301 ctgaagttct ttttgaataa taatagaagc actgctggca gatccagtcc gtttttcata
      361 acccactgta ctgataacca taatctaatc agtagaaatt gagtcgaaaa taagcactac
      421 tccatacagg ataattagag gtcagtttga ttattcacaa ttcattatca gcattttctg
      481 tttctgacga aatcaacatg aaaagaacca tatatgataa ttattataat aacggcttta
      541 attggaatac atatattaca acgtattata tataattggt attctgggaa ctatattctc
      601 aaaatacagt agaaacgagg tatgtttctg gtggaaagga cagtgggatt aaaaagtaag
      661 aattgataaa aaaacgccag caacacatag tgccggcgga gggaatcccc catggagaaa
      721 atgtgatgcc tagagcacca tgaatatata attaaaaata gttagagttg tcaatacttt
      781 cacaaaaata attttcgtag tttcgtagta tagaaagata tttttatgca tgacctacct
      841 gaatttgctc cgggtagagg ttataaataa aaattgaatc acgacaaaca caatattcat
      901 agtatggcga tgcctacgcc agcaagaata gcgccaataa tgttgggaat ataatagaca
      961 ccagacgcac aggcatctca tctcttaaca aataataatc aaggtattta cttttgcctc
     1021 gccagctgtt tacgcctaaa gcacacaaga ttaaccattc aataacaaat ggcaatatcc
     1081 atagccatac gactttacct tgtaatgttc ggtatttctt tataattatt ctgggaaatc
     1141 tatcatttat ttttaaaatc aaattatctt gttgtttaaa aataagttca catactttat
     1201 tatcttcttc cgccatcaac atctctgcat acttaaacat ttcagaacgt tcctttagca
     1261 cagaagagta attatatgtc cagttcccag gcaataatgc ttatggatat ttaactaata
     1321 atttagagaa tattttgcaa taatatttgg ctgtattcag aaatacctga aaaatcatac
     1381 tatacagccc cagggaatgg ataaagattc taacaaagca tttcaacaat atatgcttgt
     1441 taaaaatcca tatcgaaatg ccgttgcagc attaatatat gcctcattca taaaatgtaa
     1501 aagagcaagc tcgtaccagt aggggggaaa ttcattgaca tgtcctgtca atacgtacag
     1561 agccctgtca tgcttgccag cacgtaacaa ttccgtcccc agtaaccagc gtgtcccctg
     1621 attattctca gggttatatg ttagtatttt atcaatgagt ataacggcat cctgatgacg
     1681 ctgtaaatga acattcgcca gaattgcagc ataaagagcg caaatgcata tgccagatga
     1741 gcgtgaatat caatgatgtc aggagcatgt caggcaagac gcctgagttt gttgacgtaa
     1801 tttgtttcgt ttatttcatc actatcgtat gcgtcaagag ttttttcaaa ctcatcccga
     1861 aatgggccaa gcctggtttc aggaaataag aaatacccct agttattgtt cacttaaaag
     1921 tataatttgt aattcaaatc ctgagcctgt aaaacgggga gtaacatatt ctattctgaa
     1981 gagaataaaa gtcgtgatgc gatgtatcaa gcccggattg taatcccaga ttaacataga
     2041 tcacaaaaca acttattctt cactaacgtg aagataaata tcgttgtatg ccttatcacg
     2101 actacgaata ccagcaagat acaactgaca tacggaaaga taccatgttt tttactcccg
     2161 aaaataacgc taaaaagcta cttccccatc gtttgtcctt agtattgtca gcgccaacaa
     2221 tgtgggctga catgataaag ctgtctagga aaacgttcgc ctcctcagcg gacaatccaa
     2281 atggtgattg tccctattaa acgtttattt tgaa
//
LOCUS       DAJTMC010000292         2282 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.297, whole genome shotgun sequence.
ACCESSION   DAJTMC010000292 DAJTMC010000000
VERSION     DAJTMC010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.297"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            <1..184
                     /locus_tag="OO130_004169"
     CDS             <1..184
                     /locus_tag="OO130_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HCR5367339.1"
                     /translation="IYQPVYSPWVNHVERLWQALHDTITRNHQCRSMWQLLKKVRHFM
                     ETVSPFPGGKHGLAKV"
     gene            complement(204..455)
                     /locus_tag="OO130_004170"
     CDS             complement(204..455)
                     /locus_tag="OO130_004170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5367340.1"
                     /translation="MPGTTTAMSINFIGMTARTMNSNGSHGKPQIPVDYQKLLSIEDI
                     TFCRNRWGNIGENALRRVAVGKKLSFFGSDRGGENAAII"
     gene            378..1068
                     /locus_tag="OO130_004171"
                     /pseudo
     CDS             378..1068
                     /locus_tag="OO130_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(1085..1264)
                     /locus_tag="OO130_004172"
                     /pseudo
     CDS             complement(1085..1264)
                     /locus_tag="OO130_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1594..>2282
                     /gene="ipaH7.8"
                     /locus_tag="OO130_004173"
     CDS             1594..>2282
                     /gene="ipaH7.8"
                     /locus_tag="OO130_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH7.8"
                     /protein_id="HCR5367341.1"
                     /translation="MFSVNNTHSSVSCSPSINSNSTSNEHYLRILTEWEKNSSPGEER
                     GIAFNRLSQCFQNQEAVLNLSDLNLTSLPELPKHISALIVENNKLTSLPKLPAFLKEL
                     NADNNRLSVIPELPESLTTLSVRSNQLENLPVLPNHLTSLFVENNRLYNLPALPEKLK
                     FLHVYYNRLTTLPDLPDKLEILCAQRNNLVTFPQFSDRNNIRQKEYYFHFNQITTLPE
                     SFSQLDSSYRI"
BASE COUNT          675 a          515 c          429 g          663 t
ORIGIN      
        1 catttatcag ccggtttact cgccatgggt gaatcatgtt gaacggctat ggcaggcact
       61 tcacgacaca ataacgcgta atcatcagtg ccgctcaatg tggcaactgt tgaaaaaagt
      121 tcgccatttt atggaaaccg tcagcccatt ccccggaggc aaacatgggc tggcaaaagt
      181 gtagcggtat taagcgcagc tatttagatt attgccgcat tttctccacc acgatcagag
      241 ccgaaaaaag ataatttttt tccaactgcc acccggcgta atgcgttttc accaatgtta
      301 ccccacctat tgcggcaaaa ggtaatgtct tcaatcgata gaagtttctg gtaatctact
      361 ggtatttgag gttttccatg actaccgtta ctgttcattg tccgcgctgt cattccgatg
      421 aagtttatcg acatggccgt agttgttccc ggcatgagcg tttccgttgt cgctcatgta
      481 aacgtgtttt ccagctcact tactcttatg aagccagaaa actcggcgtt aaggagcaga
      541 tcgttgaaat ggcacataac ggcgcagggg gctgttatac cgccagaaca ctgaaaatcg
      601 gcatcaatac cgtcatacgg acattaaaaa gctctcgccc cggcggataa catctgcgcc
      661 agtcgctcac gctgatgttg cccttatctg tgaacttgat gagcagtggc gcttcgttgg
      721 cagcaaggcg caacaacact ggccctggta tgcgtacaac actaaaactg gcggtgtgtt
      781 ggcttacact tgcgggccac ggaatgatga gacctgtcgg gaactgttag ccctgttgac
      841 tccgttctgt atcggcatgg tgaccagcga tgactggggc agctacgcca gagaagtgcc
      901 ggaggagaaa cacctgaccg gcaaaatctt cacgcaacgt atgaacgtaa caacctgacc
      961 ctccgccccc gcatcaagcg gctggcacgt aaaacaatct gcttctcgcg ttccgtggaa
     1021 atccacgaaa aagtcatcgg ttccttcatt gaaaagcaca tattctaatg attggaagta
     1081 ctacccggca aaactcattc agtgcattcc agtgattcag gatatagttg aacgccttta
     1141 ccatttcagc atggtttgac aaagttttcc tctggagctt tatccagtca tacaaggact
     1201 tcattcgcgg cacacatttc tcctgcctta ccgcaagccg tttttctgcc ggcctccctc
     1261 atattaaatg tgtatcgttt ttttacagta attttaattt atcattctat aataggaata
     1321 aatgagcttg gctaaaatat gatgtaaaac aaatatactt actcggatta catttccttg
     1381 ctcactggaa tgttgaattc agtgttatta ttgactgcag tcacaataaa aaaaagaatt
     1441 ccatagaaaa cctcaacaaa taaattacaa ctaacttctg ttatgtgtaa aatggaaact
     1501 attaaaactt aatatcgaaa atggtaagtg aaatttgtat aaatatacaa ttttaaatat
     1561 ttattctcac aaatataagg ttgccctagc attatgttct ctgtaaataa tacacactca
     1621 tcagtttctt gctccccctc tattaactca aactcaacca gtaatgaaca ttatctgaga
     1681 atcctgactg aatgggaaaa gaactcttct cccggggaag agcgaggcat tgcttttaac
     1741 agactctccc agtgctttca gaatcaagaa gcagtattaa atttatcaga cctaaatttg
     1801 acgtctcttc ccgaattacc aaagcatatt tctgctttga ttgtagaaaa taataaatta
     1861 acatcattgc caaagctgcc tgcatttctc aaagaactta atgctgataa taacaggctt
     1921 tctgtgatac cagaacttcc tgagtcatta acaactttaa gtgttcgttc taatcaactg
     1981 gaaaaccttc ctgttttgcc aaaccattta acatcattat ttgttgaaaa taacaggcta
     2041 tataatttac cggctcttcc cgaaaaattg aaatttttac atgtttatta taacaggctg
     2101 acaacattac ccgacttacc ggataaactg gaaattctct gtgctcagcg caataatctg
     2161 gttacttttc ctcaattttc tgatagaaac aatatcagac aaaaggaata ttattttcat
     2221 tttaatcaga taaccactct tccggagagt ttttcacaat tagattcaag ttacaggatt
     2281 aa
//
LOCUS       DAJTMC010000293         2276 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.298, whole genome shotgun sequence.
ACCESSION   DAJTMC010000293 DAJTMC010000000
VERSION     DAJTMC010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2276
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.298"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(<1..350)
                     /locus_tag="OO130_004174"
     CDS             complement(<1..350)
                     /locus_tag="OO130_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HCR5367342.1"
                     /translation="MKIKSISKAVLLFALLTSTSFAAGKNVNVEFRKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRN"
     gene            592..885
                     /locus_tag="OO130_004175"
     CDS             592..885
                     /locus_tag="OO130_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HCR5367343.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            957..1616
                     /locus_tag="OO130_004176"
     CDS             957..1616
                     /locus_tag="OO130_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HCR5367344.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            1708..2154
                     /locus_tag="OO130_004177"
     CDS             1708..2154
                     /locus_tag="OO130_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HCR5367345.1"
                     /translation="MNDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PVWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
BASE COUNT          594 a          490 c          585 g          607 t
ORIGIN      
        1 tcgtttcggg tttgcagcac tcgcagctca tatttgccag aagcagggag tgagtattgt
       61 ccgtgactat cgagttctgg ggaatatctg gataaatcaa cagaatcatc gattcctggc
      121 ccgaagaggt aagtatcagc gccttcatta gagatactca catgtacttt ctgacctttt
      181 ttggcataga aggtatatgt atcgtaatcg tatcccttta tttcgccgga gtactgagcg
      241 ctgctatggc cttttctgaa ctcaacattg acattcttac ccgcagcaaa gctcgtggag
      301 gttagcagtg caaacagcaa tacagcctta ctgatgctct tgattttcat tggtagtctc
      361 ctgttcagaa tgagtacgat gaactgttat aatataacaa tccctaacgg gagaattcca
      421 gtggcttttc tgcgttcggt aaaatcccgg cgcggcattt tttgccggtg ggattactta
      481 taaaggggca tggactcaac aaaccgcaga aatctacagc tggctggctt gtggcgcggg
      541 tttcattgat gttagcttat gccaaaacct ggcatcttaa aaagtaaaag tatgttttgt
      601 gtgatttatc gaagcagcaa gcgtgaccag acctatttat atgtcgaaaa aaaagacgat
      661 ttttcgcgtg ttcctgagga actgatgaaa ggttttggtc agcctcagtt agcgatgatt
      721 ctgccgttgg atgggcggaa gaaactggtc aatgctgata ttgaaaaggt taaactggca
      781 ttaaccgagc aaggttacta tttgcagtta ccgccaccac ccgaagattt gctgaagcaa
      841 catctttccg tcatggggca gaaaacagac gacacgaaca aataaccgat atccggcggt
      901 ggcattatct ttgtcggcgc gggttttcat atccacgata aggtgagggg aacgttatgt
      961 atcaacatca caactggcaa ggtgcgctgc tggattatcc ggtgagtaaa gtagtctgtg
     1021 ttggcagtaa ctatgccaaa catattaaag agatgggcag cgcagtgccc gaagagccag
     1081 tgctgtttat taaaccagaa acggcactgt gcgatctgag gcagccattg gcgatcccat
     1141 ccgatttcgg ttcagttcat catgaagtcg aactggcggt gttgattggt gcgacgctgc
     1201 gtcaggcaac ggaagagcat gtccgcaaag ccattgccgg ttacggcgtg gcgcttgatc
     1261 tgacattgcg tgatgttcag ggaaaaatga agaaagccgg gcagccgtgg gaaaaggcta
     1321 aagcgtttga taactcttgt ccgctttccg ggtttatccc cgcggcggaa tttaccggcg
     1381 atccacaaaa tacaacgctg ggcctgagcg taaacggcga acaacgccag caaggtacga
     1441 ctgcggacat gatccataaa atcgttccgt tgatcgctta tatgagcaag ttttttaccc
     1501 tcaaggccgg tgacgttgtg ttgacaggca cgcctgatgg cgtcggcccg ttgcaaagcg
     1561 gtgatgagct gacagtcact ttcgatgggc attctttgac aactcgcgtt ttgtaatact
     1621 ttttgccgcc tgaaagcggc ggcaaaactt gcatcgctgt gccagactgg ttataaggtg
     1681 cgttttaacg taatggcgga acacctgatg aacgatgtac ctttctggca aagtaaaacc
     1741 ctggacgaaa tgagcgatgc ggaatgggag tcgttgtgtg atggttgcgg tcagtgttgc
     1801 ctgcataaac tgatggatga agacaccgac gaaatctact tcactaacgt cgcctgtcgc
     1861 cagctcaata ttaaaacctg tcaatgtcgg aactacgaac gtcgtttcga gtttgaaccc
     1921 gactgcatta aattaacccg tgaaaatctg ccaacattcg aatggctgcc aatgacctgc
     1981 gcttatcgtt tgctggcgga aggtaaagat ttacctgtgt ggcatccgct acttactggt
     2041 tcgaaagcgg caatgcatgg tgaacgtatc tctgtgcgtc atatcgcagt gaaagaatca
     2101 gaagtgattg actggcagga tcatatctta aataaacctg actgggcgca gtgataaatt
     2161 taatagatgt tgatgggggc tttcttaata tacgtctgga tgctcgccat ttcgtagccc
     2221 tgtttttgaa agcgctctgt cataatgaga gttacccgat accttattat caacag
//
LOCUS       DAJTMC010000294         2263 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.299, whole genome shotgun sequence.
ACCESSION   DAJTMC010000294 DAJTMC010000000
VERSION     DAJTMC010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2263
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.299"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(26..2254)
                     /gene="pdeF"
                     /locus_tag="OO130_004178"
     CDS             complement(26..2254)
                     /gene="pdeF"
                     /locus_tag="OO130_004178"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HCR5367346.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPFLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGIALGIFVRKYAELGFYETLSLTANFIIIIIIIIII
                     IIIIIIILCWGGYRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGL
                     LASRENLVGVMPFNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQ
                     VDAKVTKKEFALWLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYK
                     LISLLWAVVLMISIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAV
                     VRRIQRLAYVDPVVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYK
                     QKLSHWLSPLLEPGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQ
                     PQIGVSYCYVRSPVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAM
                     MNRLQQALEHNHFFLMAQPITGMRGDVYHEILLRMKGENDELISPDSFLPVAHEFGLS
                     SSIDMWVIEHTLQFMAENRAKMPAHRFAINLSPTSVCQARFPFEVSQLLAKYQIEAWQ
                     LIFEVTESNALTNVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDG
                     SFIRNIVSNSLDYQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIG
                     KP"
BASE COUNT          685 a          532 c          504 g          542 t
ORIGIN      
        1 tttcattcaa cgtatcaatt aacggctacg gcttaccaat aagataaccc tgcatataat
       61 cgatccccaa agagagcacc gcctcgcgga tctcttcgtt ttcaacgtac tctgccacta
      121 cccgcatttt cttcattcgc gccaggtggc aaatcgatgc cactatctga taatccagac
      181 tatttgacac aatattgcgg ataaaactgc cgtcaatttt aagcagatcg gcattcacat
      241 ttttaagccg tgcatagctg gcgtagccgg tgccgaaatc atcaatcgca atctggcagc
      301 ctaattcctg aagatgctgc aaggtgattt gcgcctgctt aacattggtc agagcattac
      361 tttcggtgac ttcaaaaata agttgccacg cttcaatctg atatttagcc agcaactgac
      421 tgacttcaaa aggaaaacga gcctgacata ccgaggttgg agacagatta atagcaaaac
      481 ggtgagcggg catcttcgct ctgttttcag ccataaattg cagcgtatgc tcaatgaccc
      541 acatgtcgat actcgacgat aaaccaaatt cgtgcgcgac cggcaagaag ctatcggggc
      601 tgatcagttc atcattctca cctttcatgc gcagaagaat ttcatggtaa acatcaccac
      661 gcataccggt aatcggctgg gccatcagga aaaaatggtt gtgttccagc gcctgctgta
      721 gtcgattcat catcgcgact ttatctttca attcgcgttg caaatacatt gccccgcgac
      781 gctgcatatt ttccggggcg ttggtcacga tggaaagttc ggcgaccgta tttagctctc
      841 ccagcagcag gtagatatga ttcactggcg agcgcacata gcagtaactg acgccaatct
      901 gcggttgcat cggcatgcca tcccaaaaga aacgaaattg cttgagatgg ctatccagtg
      961 cggtaatgcg ctcctggtgc gattctgtat tcagtcgcag cgcgagatcg ttacccgaaa
     1021 gctgataaac atcttcaccc ggttccagca agggtgacag ccagtgagaa agtttttgct
     1081 tgtattgaat ccgcagcatg atgccatagt tcttaaccag catttccatg ccagggatgc
     1141 gtaaataaca aagcgcagac cagggggcat cacgcaacgc gcgattcagg gcgcgaacat
     1201 ttggcagatg aaccaccggg tccacatacg caagccgctg aatgcgtctg actaccgctc
     1261 tctgacgggt tgccagtaca gccatataat tgacaataaa agagaatacc agataactgg
     1321 aggaggttat ggtcagctgc gtggtatagc caggataaat gggaatgtaa ttttgatagc
     1381 tgtggatgct gatcatcaac acgaccgccc agagcagaga aatcagctta taaccatagc
     1441 gcatcgctcc ccacatcatc aggggcagca ataatgacaa ggtataattg gtgctaaaaa
     1501 ttgtgctttt ttcatttaac ggcatgcata acagcaacaa taaagcacct aatgccagta
     1561 gccagagcgc gaactctttt ttggtgactt tggcatcaac ctgctgtttt aattgcgaat
     1621 aatagctacg taaataaaat ggatttcgca ctacccgaat gatgaagtag cacagcggga
     1681 caccgatcag attacccacc agtaaggcct gataattgat taaagtcccg aggttaaaag
     1741 gcatgacacc gaccagattt tctctgctcg ccaataatcc tacaaatgca gcaaactgga
     1801 aaagtatcag aaacagcgtt gcaggaaaca caatctgcca gaatatacgc tgggaaatta
     1861 aacgggtatc accatgtgaa acgttgttac gccggggagt aaagaccctg taaccgcccc
     1921 aacagagaat gataatgata atgataatga taatgataat gataatgata atgataataa
     1981 aattagccgt taatgatagc gtttcgtaaa aacccagctc tgcatatttg cggacaaaaa
     2041 tccctaaggc gatccctggc aatgccgccc agctgaaaaa catcatcatg ctgatcatca
     2101 gtgccagagg cagataaaag agaaaaacct caccggaaga aatatgcgcg aaagtattaa
     2161 tgtgggcgaa aatgggcaag attaaaaaag gcaggaacag cggaagcccc caccatttat
     2221 cacgtatttt tatataagtt gcattcagtt tcatagatgc tca
//
LOCUS       DAJTMC010000295         2260 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.300, whole genome shotgun sequence.
ACCESSION   DAJTMC010000295 DAJTMC010000000
VERSION     DAJTMC010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2260
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.300"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            111..432
                     /locus_tag="OO130_004179"
                     /pseudo
     CDS             111..432
                     /locus_tag="OO130_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232281.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(466..672)
                     /locus_tag="OO130_004180"
     CDS             complement(466..672)
                     /locus_tag="OO130_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5367347.1"
                     /translation="MTVSYKITYEHRDGDQIRMEEVLFHSEHEPTKDEIHDAVRKDTV
                     RFNSPSNGVTAVSGFKVISVVPVP"
     gene            complement(1170..1243)
                     /locus_tag="OO130_004181"
     tRNA            complement(1170..1243)
                     /locus_tag="OO130_004181"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1209..1211),aa:Gly,seq:ccc)
     gene            complement(1322..2077)
                     /locus_tag="OO130_004182"
     CDS             complement(1322..2077)
                     /locus_tag="OO130_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HCR5367348.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKTGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
BASE COUNT          603 a          530 c          491 g          636 t
ORIGIN      
        1 tcatacacta aatcagtaag ttggcagcat taccaatttt gctaagttaa tgcattaatc
       61 taaaaacgct ttaaatcaat acataaccat tatctcttgg gattatttac atgcgcaaaa
      121 gtttaatgct gttacttgct tcaacattga ttatttctgg atgtgcaaca aaaaaacaac
      181 tcgttccaac tggtggaagc aaatcagacg gcacagttag gatgagttat tcttacggta
      241 tgtttgaaag tcctgttgta gatccacaac aaggcatgaa tgcagccaaa gcgcgttgtg
      301 ccgcttaggg gatattcagg agccgagcct tttggtggat tcacttctca atgttcacaa
      361 ccttcttcat ctggttgtat gcaaacactt gtgactatcg aatatcaatg cactggcgat
      421 attaagaagt aataattagg ggcttccagc cccttctata accattcatg gaacagggac
      481 aaccgaaatc actttaaacc ctgaaacagc cgttactcca ttagatggag aattaaatct
      541 cacagtatct tttcttactg catcatgaat ttcatctttt gttggttcat gctctgaatg
      601 gaataaaacc tcttccattc taatttgatc accgtctcta tgctcataag taatcttgta
      661 gctaacagtc atatcaacct catgcagggg aattgtgaga ttattataga caatagaaac
      721 cataaagccc cgttggtgat acagcaacaa gcgggagcgt agcgacttca tcagattgtc
      781 agaaagatca caccttatca ggataaccgt gtacccgttg ggagcgtggc tgaaacgctg
      841 atagagcttg catatggttt gcaaggacca atatcaccat aaacctgttt acgcagccac
      901 aaggccgcac atcgggctta aatgcgtgtg ttcagtctgt cgatgacatt acaatgccag
      961 gatgggcttt atcttcacgt tgtgctatcg cggtgtgaga ggtgcaactg ccgccaacgt
     1021 taggttatac tgtcatctca ccgtgtaact taacgtgaca gttactaagg gcggatcaca
     1081 atggtagaac tcctaagcaa attaaatagg taactgttta agttgctgac agagaagact
     1141 tttttagtct tctccacctg gggagattct ggagcgggcg aagggaatcg aaccctcgta
     1201 tagagcttgg gaagctctcg ttctaccatt gaactacgcc cgcttcgaga tgcgtaaggc
     1261 attataaacc ttacgctctc cttagcaagt gccgcgctgc tgactgctga ttaattcgcc
     1321 atcagcattt tggcttgctg ccctgcggcg gcaagtaacg tagcggatca attgccgttg
     1381 cacggtaacg aatctggaaa tgcaggcgaa cagatgccgc atccgtgctc cccatagtgg
     1441 cgattttttg cccagtcttc acgctttgcc cattatttac cagcatcgtg tcattatggg
     1501 cgtaagccgt aatgtaatct tcactgtgtt taatcatgat gagattaccg tagccacgca
     1561 gctggttgcc cacatacacc acctttcctg cacccgcggc gtaaataggt gtaccccgtg
     1621 gagctgagat atcaatccct ttattgccgc catctgctgt cgaatacggc atgataactt
     1681 tccctgtcgt tggccataac caacaacgtt gccctactgg cggccaggaa gatttcggta
     1741 ccgctgatga cggtgtaacc gatgcggttt tggtcgttga tttggcggtt gatttacgtg
     1801 tactactgct acttttcgcc ccacccagtt ttagtttctg accaacttca atggtgtaag
     1861 ggggggaaat gccgttcagt cgcgccagtt cttttacgct ggttcccgtg gtgcgcgaaa
     1921 tacgatatag cgtatccccc cgtttcacgg tgtaaacgga gccggaatat gttcctgtat
     1981 cggatgattt gctacccgaa cagcccgcca aaagcagtcc aaccgataac aacatcacga
     2041 tacccagaga ttttttattc aggcgtcccg cactcaaaat ctttcctcgc ttaaaaaaca
     2101 agacgcctta tcatagcagc cagcctcttg atacccaatc tcttgcttct ggaaaaacgt
     2161 ggtaatagag aaagaggtta atttcatttg gcaaagggaa gtatcatcag caaaatattc
     2221 gctgtagagg tatcgaactt cctgactttt taccctattt
//
LOCUS       DAJTMC010000296         2215 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.301, whole genome shotgun sequence.
ACCESSION   DAJTMC010000296 DAJTMC010000000
VERSION     DAJTMC010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2215
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.301"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(<1..709)
                     /gene="ipaH9.8"
                     /locus_tag="OO130_004183"
     CDS             complement(<1..709)
                     /gene="ipaH9.8"
                     /locus_tag="OO130_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH9.8"
                     /protein_id="HCR5367349.1"
                     /translation="MLPINNNFSLPQNSFYNTISGTYADYFSAWDKWEKQALPGEERD
                     EAVSRLKECLINNSDELRLDRLNLSSLPDNLPAQITLLNVSYNQLTNLPELPVTLKKL
                     YSASNKLSELPVLPPALESLQVQHNELENLPALPDSLLTMNISYNEIVSLPSLPQALK
                     NLRATRNFLTELPAFSEGNNPVVREYFFDRNQISHIPESILNLRNECSIHISDNPLSS
                     HALQALQRLTSSPDYHGP"
     gene            complement(1102..1407)
                     /gene="ccdB"
                     /locus_tag="OO130_004184"
     CDS             complement(1102..1407)
                     /gene="ccdB"
                     /locus_tag="OO130_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858358.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HCR5367350.1"
                     /translation="MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHVGDESWRMMTTDMASVPIFVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            complement(1409..1627)
                     /gene="ccdA"
                     /locus_tag="OO130_004185"
     CDS             complement(1409..1627)
                     /gene="ccdA"
                     /locus_tag="OO130_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HCR5367351.1"
                     /translation="MKQRITVTIDSDSYQLLKSANVNISGLVNTAMQKEARRLRAERW
                     QAENQQGMAEIARFIEMNGSFADENRDW"
BASE COUNT          588 a          465 c          522 g          640 t
ORIGIN      
        1 gtgggccgtg gtagtccggc gaagaggtta atctttgcag ggcttgcaga gcatgggatg
       61 ataatgggtt atcactaata tgtattgaac attcattcct cagattaaga atgctttccg
      121 ggatatgact tatctgattt ctatcaaaaa aatactctct gacaacggga ttatttccct
      181 cagaaaatgc tggtagctca gtgaggaaat tacgggtcgc tctgagattt ttaagagcct
      241 gtgggagcga tggtaaggag actatttcgt tatagctgat attcatagtc aataacgaat
      301 cgggtaaagc tggcaggttt tccagctcat tgtgttgtac ctgaagtgac tccagcgcag
      361 gaggtagcac gggcaattct gataatttat tgctggcgga atataatttt tttagcgtaa
      421 caggcagttc aggtaggtta gttaattgat tatatgatac attgagcagc gttatctgag
      481 ctggtaagtt gtcaggtagc gaggacagat ttaaacggtc cagtcgaagt tcatcggaat
      541 tattgataag acattcttta agtcgggaga cagcctcatc acgctcttca ccggggagcg
      601 cttgtttttc ccatttatcc catgctgaaa agtaatcagc atatgtaccg gaaatagtgt
      661 tataaaaaga attttggggc aatgaaaagt tattatttat cggtaacatg atgggcatcc
      721 agttaaagcc ggtggacaaa tggctttgtt aaaagaaata agcaggagtt tttatgaagt
      781 tcattccatg aaatgaactt cagcactttt ttattataat aaggataaac aaaattggct
      841 gtaaaaaaac gatactgttt cagttaaccg tgaatggcct gtttgcaccg tattgttctt
      901 gtttttcggt tagttaaaca tcgtacttca tatttgaacg gtaagcggct cgtcagaacc
      961 gtattgatat ttactgagag gtcagatcaa ctttccaggg caacagatcg cgtacccagt
     1021 ttgccggcca gtcttggata tgctcaatga cgtaacgcag ccattttgtc aacgccacga
     1081 tttttgaccc tagcctgaca tttatattcc ccagaacatc aggttaatgg cgtttttgat
     1141 gtcattttcg cggtggctga gatcagccac ttcttccccg ataacgaaga tcggcacact
     1201 ggccatatca gtggtcatca tgcgccagct ttcatccccg acatgcacca ccgggtaaag
     1261 ctcacgggag actttatctg acagcaggcg ggcactggcc agggggatca ccatccgtcg
     1321 tccgggcgtg tcaataatgt cactctgtac atccacaaac agacgataac gactctctct
     1381 tttataggcg taaaccttaa actgcatttc accagtccct gttctcatcg gcaaaagagc
     1441 cgttcatttc aataaaacgg gcgatctcag ccatcccctg ctgattttca gcctgccagc
     1501 gttccgcacg cagacggcgg gcctcttttt gcatggccgt atttaccagg cctgagatat
     1561 tcacgtttgc ggatttaagt aactgatagc tgtcactgtc tatagtgaca gtaatacgct
     1621 gcttcatgat aagccctcaa aaacatactt atgatatgta tatgagtata tattaatggt
     1681 acaaaccatc aatatccata aaaagtacac cattaattca ctataagttt ttaatacaat
     1741 tttttctgct gcgatttttt tatacgataa ttagcatact tataacaccg taagtaaatt
     1801 acatgaaagc aggcacagcg tgatccgatt tttctgcgta taacgatttt ccggaagtgt
     1861 aatacggttg cgccccgggc cgggcaccct tttctgttgt tgtgtacggg cccacagaga
     1921 atggtaccgg aggcgggctc agaagtactg agtcgggtga tcgcgtcagg ccgcgaagta
     1981 ctgacacggg tattggtgac tggtgatgaa gtactgcccc gggtatgatt tcaggctgat
     2041 tcaggttctg attctctgtc agtccgcgtg gttgctggcc tggctgattt cctgttgtca
     2101 ggcgttcaac aaccgactgc aggttatagc ccggttcacg aaccacggca gcctggcggc
     2161 tgcaaatcgg ggtgtggcag aatatgagct ttcagagtaa ccatccagaa tgagt
//
LOCUS       DAJTMC010000297         2145 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.302, whole genome shotgun sequence.
ACCESSION   DAJTMC010000297 DAJTMC010000000
VERSION     DAJTMC010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2145
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.302"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            1..234
                     /locus_tag="OO130_004186"
                     /pseudo
     CDS             1..234
                     /locus_tag="OO130_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            655..1233
                     /locus_tag="OO130_004187"
     CDS             655..1233
                     /locus_tag="OO130_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5367352.1"
                     /translation="MKVSFKSLGYIFHDIYNKKHTIDEFNDVVRKAVLSGKINELNAC
                     HKVAIFLAEKDNEITKKDKAKIIDTLTENYSIEFQQLMNISERTLNSSLYITPGESGF
                     VSFVNREGKICHTAYVKSSDNSMAYYHANYSSIDKYITDMCGLICMRHIESTCIIFYM
                     LDEKVLSAIAEFMNEKGWRAAFCSAKDLYKCV"
     gene            complement(1342..1600)
                     /locus_tag="OO130_004188"
                     /pseudo
     CDS             complement(1342..1600)
                     /locus_tag="OO130_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            1584..2145
                     /locus_tag="OO130_004189"
                     /pseudo
     CDS             1584..2145
                     /locus_tag="OO130_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343760.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          687 a          393 c          529 g          536 t
ORIGIN      
        1 actgtcgata tgaaaaagcg agcatcatac tgacgtcaaa caaaggggtc gcagactggg
       61 gagaaatgtt cggagatcac gtgctggcaa cagcgatcct agatcggctg ctacacgctg
      121 aatatcaaag gagagagtta ccggttaaaa gagaaacgta aagctggagt gctgaccaaa
      181 aacacaacgc caatcagtga tgatgaaatg gtggaaaacg gacagcatca gtaacgaaag
      241 tatcttagca ggcatgaaaa tcgtccgcat cccctaaacg gtcaaaatgc ttctgccgtg
      301 aaggagggag aatgccatct catcacttat atctggcgaa tgaaacacag atggtcggcc
      361 ttgcccccaa acacacggcg gtgcaaaatg gaatggcaga aaacttcgtc aaaacgatga
      421 aatatgatta aatcagtgtt atgttcgaac ctgacgtgtt aacagcagta aagtagtctc
      481 gcgaaggtgt tcaaacatta taatgaatgg catcctcata gtgcgcaggg ttatcgcttg
      541 ctgcggaaat atctgaagcg acgtaccagt aatggggaaa atgatagctg gtttctgaaa
      601 atatagagga aaatgtgata tatatatcct gttaatattt aaggagaata taaaatgaaa
      661 gtctcgttta agtctttagg ctatattttt catgatatat ataataaaaa acacactatc
      721 gatgagttca acgatgtggt aagaaaagca gttttatcag gtaaaattaa cgagctgaat
      781 gcatgtcata aagtggcaat ttttcttgcc gaaaaagaca atgagataac aaagaaagac
      841 aaagccaaga taatagatac attaactgaa aactattcca ttgaatttca acaattaatg
      901 aatattagtg agcgcacgtt aaattcatct ttatatatta ctcctggtga aagtggtttt
      961 gtcagttttg ttaatcgtga aggaaaaata tgtcatactg catatgtaaa atcatcagat
     1021 aatagcatgg catattacca tgcaaattat agcagcattg acaaatatat tacggatatg
     1081 tgcggcctta tttgtatgag gcatattgag tctacttgca ttatttttta tatgcttgat
     1141 gaaaaagttt tatctgccat tgctgaattt atgaatgaaa aaggatggcg ggctgcgttt
     1201 tgttcagcaa aggacttata taaatgtgtt taattttaga aaaactattt ggtaaaatcc
     1261 aacaagagtc gagaggttcg cgttgcttca gcagggcctc agcctgttca accagactca
     1321 gttgcaggtc gtgacgcaga tttagtggtg gtggttgatt gtcagatacc agccgatgat
     1381 cttatagtgc atatcatcag actttgagaa gcatatggtt ttgcgggtaa gccgcttaag
     1441 atgtgtccgc aggttcagat tatgtcgctc tatccgctga gtgcatttct tgcttataac
     1501 atgactggtt gaggacaata aagttcggta taccggctca ggcatcggtc atgtagaagg
     1561 caatgctaaa ctggctcagc aaactaagag cagcgcgcat ccacttaccc aatgcggttg
     1621 agaaaatagc cttcgatcgc tttcatgtgg cgaaacaacc gggtgaggtg gtggataaaa
     1681 cccgccagaa tgaacatccg catcttcctg ttgaaagtcg tcgtcaggca aagggaactc
     1741 gcttcctgtg gcagcacagc gataaatgga tgaccgaatc caggcaggaa aagctgatat
     1801 ggctgcgtgc gcagatgaag ctgacgagcc tgtgctgggc gctgaaagaa ctggcaaagg
     1861 atatctggag cagaccatgg agcgaggaaa gacgcaacga ctggcagaga tggctgtcgc
     1921 tggcggccaa cagtgacgtc cccatgatga agaatgtggc taaaacgata gggaaaaggt
     1981 tgtacggtat cctgaacgcc atgcgccatg gcgtatcaaa cggaaatgcg gaagcactga
     2041 acagcaagat caggctgctg agaataaaag ccaagggcta ccgcaaccgg gagcgcttta
     2101 agctgggagt gatgttccac tatgggaaac tgaatatggc gttct
//
LOCUS       DAJTMC010000298         2111 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.303, whole genome shotgun sequence.
ACCESSION   DAJTMC010000298 DAJTMC010000000
VERSION     DAJTMC010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.303"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            239..586
                     /gene="tnpB"
                     /locus_tag="OO130_004190"
     CDS             239..586
                     /gene="tnpB"
                     /locus_tag="OO130_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004966400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR5367353.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNGFNGLAAKVQTTLKDAPMSGHVF
                     IFRGRNGSQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGKVFLTPAQLAMLLEGIDW
                     RQPKRLLTSLTML"
     gene            635..>2111
                     /locus_tag="OO130_004191"
     CDS             635..>2111
                     /locus_tag="OO130_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISEc22 family transposase"
                     /protein_id="HCR5367354.1"
                     /translation="MSSSLPDDINALKRLLAEQEALNRALLEKLNEREREIDHLQAQL
                     DKLRRMNFGSRSEKVSRRIAQMEADLKALQKESDTLTGRVDDPAVQRPLRQTRTRKPF
                     PESLPRDEKRLLPAASCCPECGGSLSYPGEDAAEQLELMRSVFRVIRTVREKHACTQC
                     DAIVQAPAPSRPIERGIAGPGLLARVLISKYAEHTPLYRQSEMYGRQGVELSRSLLSG
                     WVDACCRLLSPLEEALQDYVLTDGKLHADDTPVPVLLPGNKKTKTGRLWTYVRDDRNA
                     GSTLAPAVWFAYSPDRKGIHPQTHLAGFSGVLQADAYAGFNELYRDGRITEAACWAHA
                     RRKIHDVHVRTPSALTEEALKRIGELYAIEAEIRGMTAEQRLAERQLKTKPLLKSLES
                     WLREKMKTLSRHSELAKAFAYALNQWPALTYYADDGWAEADNNIAENALRMVSLGRKN
                     YLFFGSDHGGERGALLYSLIGTCKLNGVEPESYLRYVLDVIA"
BASE COUNT          475 a          597 c          633 g          406 t
ORIGIN      
        1 atctgttgtt caaatggcga caacaatggc gcgagggaaa gctgctatta ccttcttcag
       61 agagccccca gctacttcct gtgactctcg atgcagctgc cgaacagcca gaatcgctcg
      121 cagaggatcc ggaaaccctc agtatcagct gtgaggtaac gttccggcac gggacgctcc
      181 gcttcaatgg caatgtcagc gaaaagctcc tgactctgct gatacaggaa ctgaagcgat
      241 gatcccgtta ccttccggga ccaaaatttg gctggttgcc ggtatcaccg atatgagaaa
      301 tggcttcaac ggcctggcgg caaaggtgca gacgacgctg aaagacgctc cgatgtcagg
      361 tcacgttttt atcttccgtg ggcgtaatgg cagtcaggta aagctcctct ggtctaccgg
      421 cgatggactg tgtctgctga ccaaacggct ggagcgcggc cgcttcgcct ggccgtcagc
      481 ccgggatggc aaagtgttcc tcacaccggc acagctggcg atgctccttg aaggtatcga
      541 ctggcggcag cctaaaagac tgcttacgtc cctgactatg ttgtaagcct ctttatcctg
      601 gtcgacgctg aatgagcctg gtaatatacc cggtatgagc agctcacttc ctgacgatat
      661 caatgcactg aaacgtctcc ttgccgaaca ggaggcgctg aaccgtgccc tgctggaaaa
      721 gctgaacgag cgtgaacgcg aaatagacca tctgcaggca cagctggata agctgcgccg
      781 gatgaacttc ggcagccgct ccgaaaaagt ctcccgtcgt atcgcacaga tggaagctga
      841 cctgaaggca cttcagaaag aaagtgatac ccttaccggt cgggttgacg acccggccgt
      901 gcagcgcccg ctgcgtcaaa cccgcacccg caaaccgttc cccgaatcac tcccccgcga
      961 tgaaaaacgg ctgctgccgg cagcgtcatg ctgcccggaa tgtggaggct cactgagcta
     1021 tccgggtgag gatgccgccg aacagctgga gttgatgcgc agcgtcttcc gggttatccg
     1081 gactgtacgt gaaaagcatg cctgtactca gtgcgatgcc atcgtgcagg cccccgcgcc
     1141 ttcacggccc atcgagcggg gtatcgcagg accggggctg ctggcccgcg tgctgatctc
     1201 aaagtatgca gagcacaccc cgctgtaccg ccagtctgaa atgtacggcc gccagggcgt
     1261 ggagctgagt cgttcactgc tgtcgggctg ggtggatgca tgctgccggc tactgtcacc
     1321 gctggaagaa gcgcttcagg actatgtgct gactgacggt aagctccatg ctgatgacac
     1381 gcctgtcccg gtgctgttgc caggcaataa gaaaacgaag accgggcggt tatggaccta
     1441 cgttcgtgac gaccgtaacg ccgggtcaac gctggcgccg gcggtgtggt tcgcttacag
     1501 cccggacaga aaaggcatcc atccgcagac ccatcttgcg gggttcagtg gtgtactgca
     1561 ggcggatgca tacgccgggt tcaacgagct gtaccgggat ggccggataa cggaagccgc
     1621 ctgttgggct cacgcccgcc gtaaaatcca cgatgtgcac gttcgcaccc cgtcagccct
     1681 gacggaggaa gcgctgaaac ggatcggcga actgtacgcc atcgaggcag agataagggg
     1741 aatgacggcg gagcagcgcc ttgccgaacg tcagttgaaa acgaaaccgc tgctgaaatc
     1801 cctggaaagc tggctgcgtg aaaagatgaa aaccctgtcg cgacactcag aactggcgaa
     1861 agcgttcgca tacgccctga accagtggcc ggcgctgacg tactatgcag atgatggctg
     1921 ggctgaggcg gacaataaca tcgctgaaaa tgcgttgcgg atggtcagtc tgggccgcaa
     1981 aaactacctg ttcttcggtt cggatcatgg aggagagcgg ggagcgctgc tgtacagcct
     2041 gatcgggacg tgcaaactga acggagtgga gccagaaagc tacctccgct atgtacttga
     2101 cgtcatagcc g
//
LOCUS       DAJTMC010000299         2100 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.304, whole genome shotgun sequence.
ACCESSION   DAJTMC010000299 DAJTMC010000000
VERSION     DAJTMC010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2100
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.304"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(32..553)
                     /locus_tag="OO130_004192"
     CDS             complement(32..553)
                     /locus_tag="OO130_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5367355.1"
                     /translation="MDVYANGQHVFRFQNGVAIALKNIQAGNAKKFTLSSANNSTKNA
                     AFYLWGNPSRPVVAELGDDSGWHFYSQRNTDGSITFAVNGQVIPLNYGNFDARYKHRT
                     EGVQDVRYGYEMYYNPGSNTVSWTFRSPSGHGLSGISISDTGRNSADNVNGVYYRPLQ
                     KLINGIWYNVASI"
     gene            complement(574..1239)
                     /locus_tag="OO130_004193"
     CDS             complement(574..1239)
                     /locus_tag="OO130_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004981765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5367356.1"
                     /translation="MHRIDTLTAVKDKFGPGKNGFTDGNLRTGRFATWLNSAMWNAIQ
                     EEICGVIEKAGIELNKEEHDQLYKAILLLVGGAINEEALLIKNNLSDVEDRDEAVENL
                     GLKPTVDKAKNAVQRDGDTMTGELKIRGVNALRIFNEAFGLIFRRSEECLHLIPTSEG
                     QGENGDIGPLRPFTINLRTGEISMSHKVSVGGGSQVNGALGIGVQNALGGIQLLSGIT
                     IQV"
     gene            complement(1226..1816)
                     /locus_tag="OO130_004194"
     CDS             complement(1226..1816)
                     /locus_tag="OO130_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2313 domain-containing protein"
                     /protein_id="HCR5367357.1"
                     /translation="MDSLQDDYTKLLYGLMQPGPAWSDTDGVLDGLAPSLVRVHQRAD
                     ELVIEIDPGQSTELIERYEELYGLPDSCSPVGTQTLRQRQQRLEAKANVAGGINEQFF
                     LDQLEALGYTGVTIEQFQHLDASPDPEWGDRWRYFWRVTLPVDAGAQWQTCTDACNTP
                     IRTWGDTVAECVINKLCPSHTVVLFSYPDEDEDAQD"
     gene            complement(1816..1974)
                     /locus_tag="OO130_004195"
     CDS             complement(1816..1974)
                     /locus_tag="OO130_004195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5367358.1"
                     /translation="MYLSRISEAISLSAGEVAHRLIVPSSDIDLGETELPVLGEITWQ
                     AYDPARSK"
BASE COUNT          532 a          585 c          447 g          536 t
ORIGIN      
        1 cctgccgtaa tattttttaa atgcaacatt gttaaatact cgctacgtta taccagatgc
       61 cattaatcag tttttgcagc ggtcggtaat acacaccgtt gacgttatcc gctgagttac
      121 ggccggtatc cgatatcgat atccctgaca acccgtgtcc cgaaggtgag cgaaacgtcc
      181 atgaaacggt gttgctgcca gggttgtaat acatttcata accataccgc acatcctgta
      241 ccccctcagt tcgatgttta tagcgggcat cgaagtttcc gtaatttaac ggaattacct
      301 gtccgtttac agcgaatgtg atactaccat cggtatttct ctggctgtag aaatgccagc
      361 cggaatcatc accaagctct gcaacaacag gcctggatgg attaccccac aaataaaacg
      421 ctgcattttt cgtggagttg ttggcgctgg ataacgtgaa ttttttagca tttccggcct
      481 gaatattttt taacgctatc gccacaccat tctggaaacg aaatacatgc tgtccattcg
      541 cataaacatc cagaatgccg tcgccgtttt gttttatacc tgtatcgtta tccccgaaag
      601 caattgaatt ccccccaggg cgttctgaac gccgataccc agcgcaccat tgacctgcga
      661 accaccgcca acagacactt tatgcgacat ggatatttca cccgtccgca gattaatggt
      721 gaacgggcgc aggggaccaa tatcgccatt ttccccctga ccttcactgg tgggaataag
      781 gtgcaggcac tcttccgaac gacgaaaaat caggccaaaa gcttcgttga aaatcctcag
      841 cgcattaaca ccacggattt tcagctctcc ggtcatggtg tcgccatcac gctgaacggc
      901 attttttgcc ttatccaccg tgggttttaa tccgaggttt tcaacagcct catccctgtc
      961 ttccacatcc gaaaggttat tttttatcag caatgcctct tcgttaattg caccgcccac
     1021 cagcaataat atagccttat atagctggtc gtgttcttct ttattcagtt ctatcccggc
     1081 cttctcaatg acaccacaga tttcctcctg aatggcattc cacatggcac tgttcagcca
     1141 ggtagcaaaa cgtcctgtgc gaagatttcc gtcagtaaat ccgttcttac ccggaccaaa
     1201 cttatctttt accgcagtca gcgtatcaat cctgtgcatc ttcatcctca tctggatagg
     1261 aaaataaaac gacggtgtgt gacggacata atttattaat cacgcactcc gcaaccgtat
     1321 ccccccacgt ccggatcggt gtgttgcagg cgtccgtgca tgtctgccac tgagcaccgg
     1381 catccaccgg caacgtcaca cgccagaaat aacgccagcg atctccccat tccggatcgg
     1441 ggcttgcatc caggtgctgg aactgttcga tcgttacgcc ggtatatccc agcgcctcaa
     1501 gctgatccag gaagaactgc tcatttatgc caccagccac attggctttt gcttcaagac
     1561 gttgctgacg ctggcgtaat gtctgggttc cgacagggga acaggaatca ggtaaaccat
     1621 acaattcttc ataacgttca atcagctctg tggactgacc gggatcgatt tcaatcacca
     1681 gttcatcagc ccgctggtga acacgcacaa gcgatggtgc cagaccgtca agtacaccgt
     1741 cggtatctga ccatgcaggc cccggctgca tcagtccgta cagcagcttt gtataatcat
     1801 cctgtaacga atccattatt tactccttgc cgggtcataa gcctgccagg tgatctcgcc
     1861 gagcaccgga agctcggtct cccccaggtc aatatccgat gaagggacga ttaaccggtg
     1921 ggccacttca ccagcagaca aactgatggc ctcactgatt ctggacagat acatacgccc
     1981 ctctggcaca ctgaggtagc ctgagtttaa cggacactcc ttcctgaaat agaatggcat
     2041 cagaaggagc taataatgag cagaaaaaac caacgttact ctaaagagtt caaagccgaa
//
LOCUS       DAJTMC010000300         2095 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.305, whole genome shotgun sequence.
ACCESSION   DAJTMC010000300 DAJTMC010000000
VERSION     DAJTMC010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.305"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            <1..116
                     /locus_tag="OO130_004196"
     CDS             <1..116
                     /locus_tag="OO130_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001362728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR5367359.1"
                     /translation="VSASSCKVEFRHGNMTLENPSPELLTVLIRELTGRGR"
     gene            113..463
                     /gene="tnpB"
                     /locus_tag="OO130_004197"
     CDS             113..463
                     /gene="tnpB"
                     /locus_tag="OO130_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017901265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR5367360.1"
                     /translation="MISLPSGTRIWLVAGITDMRKSFNGLGEQVQHVLNDNPFSGHLF
                     IFRGRRGDMIKILWADADGLCLFTRRLEEGQFIWPAVRDGKVSITRSQLAMLLDKLDW
                     RQPKTSRLNALTML"
     gene            494..2086
                     /locus_tag="OO130_004198"
     CDS             494..2086
                     /locus_tag="OO130_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HCR5367361.1"
                     /translation="MNQKYLIRIAELECQLRQKDQQLSLVEETEAFLRSALARAEEKI
                     EEDEREIEHLRAQIEKLRRMLFGTRSEKLRREVEQAEALLKQREQDSDRYSGREDDPQ
                     VPRQLRQSRHRRPLPEHLPREINRLEPEESCCPECGGELDYLGEVSAEQLELVSSALK
                     VIRTERVKKACTKCDCIVEAPAPSRPIERGIAGPGLLARVLTGKYCEHLPLYRQSEIF
                     ARQGVELSRALLSNWVDACCQLMTPLNDALYRYVMNSRKVHTDDTPVKVLAPGRKKAK
                     TGYIWTYVRDDRNAGSPEPPAVWFAYSPDHQGKHPEQHLSPFRGILQADAFNGYDRLF
                     SAEREGGALTEAGCWAHARRKVHDVYISTKSATAEEALKLIGELYAIEHEIRGLPVSE
                     RLAVRQMQSKPLLTSLYKLMQEKEHTLSKKCRLRDAFRYIRKHWVALCNFSDDGLAEA
                     DNNAAERALRAVCLGKKNFMFFGSDHGGERGALLYGLIGTCRLNGIDPEAYLRYILSV
                     LPEWPSNRVDELLPWNVALTNK"
BASE COUNT          527 a          529 c          602 g          437 t
ORIGIN      
        1 cagttagtgc cagctcctgc aaggtggagt tccgtcacgg taacatgacg ctggaaaatc
       61 cttcaccaga gctgctcaca gtgttgatcc gtgaactgac cgggagggga cgatgatctc
      121 actcccgtca ggcacccgca tctggctcgt tgctgggata accgatatgc gtaagtcttt
      181 caacgggctg ggtgaacagg tacagcatgt gctgaatgat aatcccttct ccggtcacct
      241 gttcatcttc cgtggccgac ggggtgacat gattaaaatc ctgtgggctg atgctgatgg
      301 tctgtgcctg ttcaccagac gcctggagga aggccagttt atctggcctg ctgtgcgtga
      361 cggcaaggta tccattaccc gctcgcaact ggcaatgctc ctcgataagc tggactggcg
      421 tcagccaaaa acatcccgcc ttaatgcact gacaatgttg taaaaatgtc atggccggat
      481 tataaaaacg gccatgaatc aaaaatacct cattcgcatt gcagaactgg aatgccagct
      541 ccgtcagaaa gaccagcaac tgagtctggt tgaagagacg gaggccttcc tgcgctctgc
      601 actggcccgc gccgaagaaa agatcgaaga agatgaacgg gaaatagaac atctgcgggc
      661 tcagatagaa aaactgcgcc ggatgctgtt cggtacccgt tctgaaaaac tgcgtcgtga
      721 agttgaacag gctgaggccc tgctgaaaca acgcgaacag gacagtgatc gttacagtgg
      781 gcgggaagac gatccgcagg ttccccgcca gttgcgacag tctcgtcatc gtcgcccgtt
      841 accggagcat ctgccccgcg aaataaatcg cctggagcca gaagaaagct gttgcccgga
      901 gtgtggcggt gagctggatt atctggggga agtcagcgca gaacaactgg aactggtgag
      961 cagcgctctg aaagtgatcc gcacagaacg ggtaaaaaaa gcctgtacaa aatgtgactg
     1021 catcgttgaa gcaccggcac catcccgtcc gatagagcgt ggtatcgcgg gcccggggtt
     1081 acttgcccgc gtgttaacgg gaaaatactg cgaacacctg ccactgtatc gtcagagtga
     1141 aatttttgcc cgtcagggtg tcgaactgag ccgtgcatta ctctccaact gggttgacgc
     1201 gtgctgccag ttaatgacgc cgctgaatga tgctctgtac cgttatgtaa tgaacagccg
     1261 caaagttcac actgatgaca caccagtaaa agtgctggca ccgggcagga agaaggcgaa
     1321 aacaggatat atctggacgt atgtccggga tgacaggaat gccggttcgc cagagcctcc
     1381 ggcggtctgg ttcgcctact caccggacca tcagggtaaa catccggagc agcaccttag
     1441 tcccttccgg ggtatcctgc aggcagatgc gtttaatggt tacgatcggc tgttcagtgc
     1501 cgaacgagaa ggcggcgcgt tgacggaagc aggatgctgg gctcatgcgc ggcgcaaagt
     1561 ccacgatgta tatatcagta ccaaaagcgc gacagcggaa gaagccctga aactaatcgg
     1621 tgagctgtac gccatcgagc acgaaatacg cgggttgccg gtgtctgaac gcctggcggt
     1681 caggcaaatg cagagtaaac cgctactgac ttccctgtat aagctgatgc aggagaaaga
     1741 acacacgtta tcgaaaaaat gccgtctgag agatgcgttc cggtatatca ggaagcactg
     1801 ggttgcgttg tgcaacttca gtgatgatgg tctggctgag gcggataata atgccgcgga
     1861 aagagcgctt cgtgcagtct gtctcggaaa gaaaaacttt atgttcttcg gcagcgatca
     1921 cggtggagag cgtggtgcgc tactgtacgg gctgatcggc acctgccgac tgaacggtat
     1981 cgatccggaa gcgtatctgc gctatatcct gagcgtactg ccggaatggc cttccaaccg
     2041 tgttgacgaa ctcctgccat ggaacgtagc actcaccaat aaataagcgt caata
//
LOCUS       DAJTMC010000301         2006 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.306, whole genome shotgun sequence.
ACCESSION   DAJTMC010000301 DAJTMC010000000
VERSION     DAJTMC010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.306"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            <1..1983
                     /gene="yicI"
                     /locus_tag="OO130_004199"
     CDS             <1..1983
                     /gene="yicI"
                     /locus_tag="OO130_004199"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HCR5367362.1"
                     /translation="KSGNLSARVSKGEFWSLDFLRNGERITGQDTNNQRNYMFERLDL
                     GVGETVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQ
                     SVSFEVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLW
                     LTTSFTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEG
                     MIRRLKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYD
                     FTNPDACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQWFDGSDPQKMHNHYAHIYNE
                     LVWNVLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSG
                     FGFWSHYIGSFENTAPAHVYKCWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFF
                     TQLKCRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVF
                     TEAGDVQFYLPEGRWTHLWHNDELDGRCWHKQQHSFLSLPIYVRDNTLLALGNNDQRP
                     DYAWHEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAARTGNTITVTGAGEAKNWTL
                     CLRNVVKVNGLQDGSQAESEQGLVVKPQGNALTITL"
BASE COUNT          461 a          483 c          572 g          490 t
ORIGIN      
        1 aaaagtggca acttaagcgc gcgtgtcagc aaaggtgagt tctggtcact cgattttctg
       61 cgcaacggtg aacgtattac cggtcaggac acgaataatc aacgaaatta tatgtttgag
      121 cggcttgatc ttggcgttgg cgaaacagtt tatggtctgg gagagcgttt tactgccctg
      181 gtgcgcaatg gtcagacggt agagacctgg aaccgggacg gcggcacaag tactgaacag
      241 gcgtataaaa atatcccgtt ctatatgact aaccgtggct atggggtgct ggtcaatcac
      301 ccccagagtg tctcttttga agtgggatcg gagaaagtct ccaaagtgca gttcagcgtt
      361 gagagcgaat atctcgaata ctttgttatc gacggtccga cgccgaaagc ggtacttgat
      421 cgttataccc gttttactgg tcgtccggcg ctgccgcccg catggtcctt cggcctgtgg
      481 ctaaccactt catttaccac caactacgac gaagcgacgg taaacagctt tatcgatggt
      541 atggcggaac gcaatctgcc gctgcatgtt ttccactttg actgtttctg gatgaaagcc
      601 ttccagtggt gcgattttga gtgggacccg ctgactttcc cggacccgga agggatgatc
      661 cgccgcctga aagcgaaagg gctgaaaatc tgcgtctgga ttaaccccta tatcggtcaa
      721 aaatcccccg tctttaaaga gttacaagag aaaggctatt tactcaaacg cccggacggt
      781 tcgctatggc agtgggataa atggcagcca ggtctggcga tttatgactt taccaatccg
      841 gatgcctgca aatggtacgc cgacaaactg aaaggtctgg tcgcgatggg cgttgattgc
      901 tttaagaccg actttggcga acgtatccca actgatgttc agtggtttga cggttccgat
      961 ccgcagaaaa tgcataacca ttatgcgcac atctacaacg aactggtgtg gaacgtgctc
     1021 aaggacaccg ttggtgagga agaagctgtc ttgtttgccc gctcggcctc cgtcggtgcg
     1081 cagaaattcc cggtacactg gggtggcgat tgttacgcta actacgaatc aatggcggaa
     1141 agcctgcgcg gtggtttgtc tattggcctt tcaggttttg gcttctggag ccactatatc
     1201 ggcagctttg aaaataccgc tccggcgcac gtttacaaat gctggtgcgc gtttggtttg
     1261 ctctccagcc atagccgttt acacggtagc aaatcttatc gtgtgccgtg ggcctacgat
     1321 gatgagtcct gtgatgtggt gcgcttcttc acgcaactga aatgccgcat gatgccgtat
     1381 ctgtatcgtg aagctgcgcg tgcaaacgcg cggggtacgc cgatgatgcg ggccatgatg
     1441 atggagttcc cggacgatcc ggcttgtgat taccttgacc gtcaatacat gttaggcgac
     1501 aacgtgatgg ttgcgccggt gttcactgaa gcgggcgatg tgcagttcta cctgccggaa
     1561 ggtcgctgga cacacctgtg gcacaacgat gagctcgacg gtcgttgctg gcataaacag
     1621 cagcacagct tcctgagtct gcccatttat gtgcgtgata acaccctact ggcgctgggc
     1681 aacaacgatc aacgtcccga ttacgcgtgg cacgaaggca cggcattcca cctctttaat
     1741 ctgcaagacg ggcatgaagc cgtctgtgaa gtgcccgctg ctgacggatc ggtgatcttt
     1801 actttaaaag cagcacgtac tggcaacacg attactgtga ctggtgcggg cgaggcgaag
     1861 aactggacac tgtgcctgcg caatgttgtg aaagtaaatg gtctgcaaga cggttcgcag
     1921 gctgaaagtg agcaggggct ggtggtgaag cctcaaggga atgcgctgac aattacgttg
     1981 taatctattg atggaatgcc gggtac
//
LOCUS       DAJTMC010000302         1976 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.307, whole genome shotgun sequence.
ACCESSION   DAJTMC010000302 DAJTMC010000000
VERSION     DAJTMC010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1976
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.307"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            547..1536
                     /locus_tag="OO130_004200"
     CDS             547..1536
                     /locus_tag="OO130_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001362017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HCR5367363.1"
                     /translation="MKNPKLQYLTDYSPSDAPWDAHRSVSDDVGGIYLLAAEYERYGA
                     RMASCGGLLRFGWSTLKETGETRLRLREAHFCRVRHCPVCQWRRSLMWQARFYQSLPR
                     IVADYPDARWMFLTLTVRNCAIGELGEMLNRMNAAFQRMKVRKEFLPVQGWIRTTEVT
                     RGSDGSAHPHFHTLMMAPPGMLNGKSYVRHERWVELWRECLRVDYDPNVDVRAVKPRK
                     PKDGESLACATAELVRGAVAETLKYSTKPADMVADPEWFLELTRQTHKRRFVATGGAL
                     KDVLKLDQETDADMVIGDDISEGDDDGSRIAFEWKTEVRKYRRSPTKDKTESG"
BASE COUNT          439 a          527 c          585 g          425 t
ORIGIN      
        1 aaaaaaaatt cggcgtcccg ctcactgttc ccgaactcgt caaacagaaa atcgcggggg
       61 cccctccggg gttccgattt tgtgattgac gacggcacgg aaaaaaacac tggggatggt
      121 ggttggtgtc acgcagtgag gccaaccacc tgcaagacgg tttttgtttt tcacaaaatc
      181 gctggaaaaa cctgtgagtg ttttgcgggg aaaacgcttt tgtggttgtt gtttttgctg
      241 tccgaaaatc agctcagatt ttgagcaaat ctcgcgcgcg ttccttgtat ttatacttaa
      301 gggataactc taggattcaa attggcctcc agcccttgtc ccgcaaggcc tgagggggga
      361 tttgtcggaa aatggttttg gcgttattct gtcgggagtg gttgcgttaa aagtgacccc
      421 cgcaacgacg ggcatcgttc gggggtgtga ccacctgtag aaattcgaat tagtgacacg
      481 acaacacagg tgatatggca atgataacac gtcagatgag ccatcgccca caatcgggtg
      541 gtgcgcatga aaaaccctaa actccagtac ctcactgatt attccccctc tgatgcgccc
      601 tgggacgcac acagaagcgt ttctgacgac gtgggaggga tttacctgct tgcggccgaa
      661 tatgagcgct acggggctcg tatggcctcc tgtggcggtt tactgcgttt cgggtggtct
      721 accctcaagg aaaccggaga aacgcgtctg aggctgcgtg aggcacattt ctgccgtgtc
      781 cggcactgtc ctgtctgcca gtggaggcgc tccctgatgt ggcaagcccg tttctaccag
      841 tccctgccgc gtatcgtggc ggattatccc gatgcccgct ggatgttcct gaccctgacc
      901 gtgcgcaact gtgcgattgg ggaactgggc gagatgctga accggatgaa tgcagctttt
      961 cagcgtatga aggttcgcaa ggagttcctg cctgttcagg gctggatccg cacaacggaa
     1021 gttacccgcg gcagcgacgg cagcgcacac ccgcacttcc ataccttgat gatggctcct
     1081 cctggcatgc taaatgggaa aagttacgtc aggcatgaac gctgggttga gctatggcgg
     1141 gagtgcctgc gggttgatta tgacccgaat gttgatgtcc gggcagtgaa accccgcaag
     1201 ccgaaggatg gcgagtcctt agcctgcgcg accgctgaac tggtgcgcgg cgcagtggcc
     1261 gaaaccctga aatattccac gaagccggct gacatggtgg ccgatcctga atggttcctc
     1321 gaactgaccc gccagacgca caagcgccgc tttgtggcga ccggcggcgc actcaaagac
     1381 gtgttgaaac tcgatcagga aaccgacgcc gacatggtga tcggggatga tatttccgag
     1441 ggcgacgatg acggttcacg aatcgcgttc gagtggaaaa ccgaggtcag aaagtaccgt
     1501 cgctcaccga cgaaagataa aactgaatca ggctagcggg gtgtcggggc gaagccctga
     1561 ccaggtgggg aatgtctgag cgagcgtgcg cggtccgaca ttcccacatc ctgtcccgaa
     1621 aattcccgcc gtttctcggt tttttgagcg tcgcagaccg cacgacaaac gaaacgaagt
     1681 gtagtttgta tgagtgcgca cttcgtgaga ttccccgact gccagtttgt cgatcgcgcg
     1741 gaacagctgc ggcacgaaaa cggcagaatg atttgctgtc cggttgccag aactgtgcgc
     1801 ttacgtggag ggcggcgcgg cgcaaaaact gccgacattg cgccagtgtt ccggtgacac
     1861 aaacccgcag aaatcgggac aggatgtgta atcgtataac cgcgcacgca cagccatccg
     1921 attaccacct ggtcagggct tcgccccgac accccgccaa cgcttcacgg cacgcg
//
LOCUS       DAJTMC010000303         1936 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.308, whole genome shotgun sequence.
ACCESSION   DAJTMC010000303 DAJTMC010000000
VERSION     DAJTMC010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1936
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.308"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            368..1156
                     /gene="virF"
                     /locus_tag="OO130_004201"
     CDS             368..1156
                     /gene="virF"
                     /locus_tag="OO130_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family invasion system transcriptional
                     regulator VirF"
                     /protein_id="HCR5367364.1"
                     /translation="MMDMGHKNKIDIKVRLHNYIILYAKRCSMTVSSGNETLTIDEGQ
                     IAFIERNIQINVSIKKSDSINPFEIISLDRNLLLSIIRIMEPIYSFQHSYSEEKRGLN
                     KKIFLLSEEEVSIDLFKSIKEMPFGKRKIYSLACLLSAVSDEEALYTSISIASSLSFS
                     DQIRKIVEKNIEKRWRLSDISNNLNLSEIAVRKRLESEKLTFQQILLDIRMHHAAKLL
                     LNSQSYINDVSRLIGISSPSYFIRKFNEYYGITPKKFYLYHKKF"
     gene            complement(1149..1520)
                     /locus_tag="OO130_004202"
                     /pseudo
     CDS             complement(1149..1520)
                     /locus_tag="OO130_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            1519..1936
                     /locus_tag="OO130_004203"
                     /pseudo
     CDS             1519..1936
                     /locus_tag="OO130_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          610 a          347 c          386 g          593 t
ORIGIN      
        1 aatatcaaca ccaaccggat ttgtggagcc tccagtctga aggggcttta tgcgttccgt
       61 ataggatatt atgatgctgg agttttgcga agctccactc ctttccgaac atttacacag
      121 atattgccta agaaaagtga aaccataata tggttatagt ccctttcagt gcaaatactt
      181 agcttgttgc acagagaaat agaagctgca taagctcttt cttcaaaaaa tgtaaataaa
      241 gttaaatata ggaaaaatta cttaatctat cttaataacg gaaagatttt gtatacaatc
      301 actgttacag aaatttctta gttactctgt aaacactaaa tatagtttgg tatattctgt
      361 tgaatttatg atggatatgg gacataaaaa caaaatagat ataaaggttc gcttgcataa
      421 ctatattatt ttatatgcaa aaaggtgttc aatgacggtt agctcaggca atgaaacttt
      481 gactatcgat gaagggcaaa ttgcttttat agagcgaaat atacaaataa acgtctccat
      541 aaaaaaatct gatagcatta atccatttga gattataagc cttgacagaa atttattatt
      601 aagcattatt agaataatgg aaccaattta ttcatttcaa cactcctatt ctgaggagaa
      661 aagggggtta aacaaaaaaa tattcctcct ctctgaggag gaggtttcta tcgatttgtt
      721 caaatctata aaagagatgc cttttggcaa aagaaagatc tatagtttag cttgcctttt
      781 atcagctgtt tctgatgagg aagctttata tacttcgata tcgatagctt cttctcttag
      841 tttttctgat cagataagga agattgttga aaaaaacatc gagaagagat ggcgtctttc
      901 tgatatttca aataacttga atttatcaga aatagctgtt agaaaacgat tggagagtga
      961 aaaattaaca tttcaacaaa tccttcttga tattcgcatg catcatgcag caaagctttt
     1021 attgaatagt caaagctata ttaatgatgt atcaagactt atcggaatat caagcccatc
     1081 ttattttata aggaaattta atgaatatta tggtataact ccaaagaaat tttacttata
     1141 tcataaaaaa ttttaaatgc ttcatagccc atcgctattg ccagatgggt tttccccaca
     1201 cctgatggcc ctagtaatac gatattttca ttacgttcta tgaagctgag tgagcgtaac
     1261 gactggagtt gcttctgcgg tgctctggtg gcgaatgtga agtcatactc ttcgaacgtt
     1321 ttcaccgccg ggaaggctgc cattcgggta tacatcgcct gtttacgttg atgacgtgcc
     1381 agtttttctt catgaagcag atgctccagg aagtccatat aactccattc ctggtctact
     1441 gcctgttgtg acagcgcagg cgctgcgctt atgtactgca cccattttgt tggacgatga
     1501 aatggaatag cccctaatat gtcaaagcca aaataccctt ttgaaaagcg ccttgaagtc
     1561 gtgaatcact acttcactac tgatgatggt tacaggatca tctcggcacg ttttggtgtc
     1621 cccgaaccca ggtcaggaca tgggttgccc tctatgaaaa acatggagaa aaaggcttaa
     1681 ttcccaaacc taaaggtgtt agtgctgatc cagagttgcg tattaaggtc gtgaaagctg
     1741 tgattgagca gcacatgtcc cttaatcagg ctgctgctca ctttatgctt gctggtagtg
     1801 gttctgtagc caggtggctg aaggtttatg aagagcgcgg agaagctggt ttacgcgcgc
     1861 tcaagattgg caccaaaaga aacattgcaa tatcagttga tccagaaaaa gcggcatcag
     1921 cattggagct gtcaaa
//
LOCUS       DAJTMC010000304         1880 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.309, whole genome shotgun sequence.
ACCESSION   DAJTMC010000304 DAJTMC010000000
VERSION     DAJTMC010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1880
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.309"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(28..900)
                     /locus_tag="OO130_004204"
                     /pseudo
     CDS             complement(28..900)
                     /locus_tag="OO130_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
     gene            complement(914..1054)
                     /locus_tag="OO130_004205"
     CDS             complement(914..1054)
                     /locus_tag="OO130_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5367365.1"
                     /translation="MSEENKENGFNHVKTFTKIIFIFSVSVFNDNESKITDAAVNLFI
                     QI"
     gene            1193..1864
                     /gene="queE"
                     /locus_tag="OO130_004206"
     CDS             1193..1864
                     /gene="queE"
                     /locus_tag="OO130_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HCR5367366.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYDVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
BASE COUNT          557 a          383 c          418 g          522 t
ORIGIN      
        1 tccgggatgg cattttttat ggttgagtta ccagcggccg gatgcgccgc cacctccaga
       61 agaaccgcca ccgccgctaa atccgccgcc acttgagcct ccaccggaac cgctgttgtc
      121 tgaatgataa cttttacctg atgctctttt ctgaagtaca cataaacagg taaagaccat
      181 aaatatggtg aaagtaaaaa agaaggaaaa tacagcaatg gaaaaaattt tatcggagaa
      241 caggaataaa tctaaaagat agattgctga aatacagact ccacttttac atgcgcgaca
      301 aaaattactg ccttgatgaa aaaaccagaa agggaagaac atcactgcaa atacccaaaa
      361 aatagcaaag taataatgat cactggaaga cgctgattca ctttctgaag gattagtcgg
      421 atattggtgt tgtgaagtga gttggttatt caaagcgttt atagctagct ctaatccctg
      481 agcaaatttt tgttgtttaa atgcgggtat catgttgcta cggatgatat ctccagccag
      541 agcatcggtt accttttctt ccagcccata acctacctgg atgcggacag tacgatctga
      601 ccaggcaaca atgatcagta tcccatcatt acgtttggca tctcctaaac gccaattgtc
      661 aaaaactctt gtagcatatt gttcaattgt ctcgtctttg gttgtgggaa caattaatac
      721 agcaatttgc acttgggtat tattgaaaat agattctaac ttgtgcgtca gttctttctt
      781 ttcttgtgag tttaagctac tcgtaagatc cgtaacttgt tactgtaatt gtgggacaat
      841 agttggttgg gctgcaacaa aggaacatac gaaagtgaac attagtatga aatagcgcat
      901 tataaatttc tcattaaatc tggataaata aattgacggc ggcatcggta attttagact
      961 cgttatcatt aaaaactgat acagaaaaaa taaatataat tttggtgaat gttttgacat
     1021 gattaaatcc attttcttta ttttcttctg acataatacg ttactccctt taattattaa
     1081 cgatatgttt cgcatttttc tttaaatctg catgtaacag aatgttaata aggcatgatt
     1141 gttgcggcag gatctgcaat aattaacaat tatccacttc acagagaatg ctatgcagta
     1201 cccgattaac gagatgttcc agaccctgca aggtgagggt tactttaccg gcgtccccgc
     1261 catttttatt cgtttacagg gatgcccggt tggctgtgcc tggtgcgaca ccaaacacac
     1321 ctgggaaaag cttgaggatc gggaagtctc ccttttcagc attctggcga agaccaaaga
     1381 gagtgataag tggggggcgg cgagcagtga agatttgctg gctgtcattg gtcggcaggg
     1441 atataccgcg cggcatgtgg tgattacggg tggtgagcct tgcattcatg atttgctgcc
     1501 actgactgac ctgctcgaaa agaacggttt tagctgccag atcgaaacca gtggaactca
     1561 tgaagtacgc tgcacaccga atacctgggt taccgtatcg ccaaagctga acatgcgcgg
     1621 cggctatgac gtgttgtcac aggcactgga gcgagccaac gaaatcaagc atccagtggg
     1681 gcgcgtacgc gatattgaag cactcgatga actattggca acgctgaccg atgataaacc
     1741 gcgagtcatt gcactgcagc cgattagcca aaaggatgat gccacacgtt tgtgcattga
     1801 aacctgcatt gcgcgtaatt ggcgtttgtc gatgcaaaca cataaatatc taaatattgc
     1861 ctgattaaac atttataagc
//
LOCUS       DAJTMC010000305         1837 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.310, whole genome shotgun sequence.
ACCESSION   DAJTMC010000305 DAJTMC010000000
VERSION     DAJTMC010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1837
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.310"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            <1..>1837
                     /locus_tag="OO130_004207"
     CDS             <1..>1837
                     /locus_tag="OO130_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HCR5367367.1"
                     /translation="RYEKGGKDGITMTFIGPSPSVQDKSASTDNTNINGEDMTEIEES
                     MLLPVSGQELPIRWLAQHGSEKPVTHVSRDELQALHIARAEELPAVTALAISHKTSLL
                     DSLEIRDLHKLVRDTDKVFPNPGNSDLGLITAFFEAYLDADYTDRGLLTKEWMKGNRV
                     SRITRTASGANAGGGNKTDRNPNLVHTLDTLDVEIAAATLPMDFNIYEIPGSVYRRAK
                     EVVLKKESPFKEWSAALRAPPGILDYSRAAIFALIRSAHPEFYHYPGRLQGYINAYLT
                     ETDHENPSKETLTATRHTPEKDILEEINREVVTERETEEEKPQPSDAMAGEQATTETM
                     EPDTTEHCQNAQSLDAQSQVSSANQVKVTADEVNKIMQAANISQPDADKLLAVSRGEF
                     VEGISDPNDPKWVKGIQTRDSVNQNQHESERNDQKAEQNSPNALQNEPETKQSEPVAQ
                     QEPEKVCTACGQSGGGNYPDCGAVMDDATYQKTFDEENQVEVQENDPEEMEGAEHPHK
                     ENAGSAQDHASDSKTGETADPLITVNGHHEITSTSRTCDHLMIDLETMGKNPDAPIIS
                     IGAIFFDPQTGDMGPEFSKTIDLETAGGVIDRDTIKWWLKQSREA"
BASE COUNT          561 a          465 c          448 g          363 t
ORIGIN      
        1 accgctatga aaaaggcggg aaagatggca tcacaatgac atttatcggc ccttccccct
       61 ctgttcagga caaatcagcc agcactgata ataccaacat caacggcgaa gacatgactg
      121 agattgagga gagcatgctt ctacctgtct ccggtcagga actgcccatt cgttggcttg
      181 ctcaacacgg cagcgaaaaa ccagtaacgc acgtttcacg cgacgaactc caggcattac
      241 acattgcacg ggctgaagaa ctaccggctg ttactgccct ggctatttcg cataaaacca
      301 gtctgctcga ctcgctggag attcgcgacc tccacaaact ggttcgtgac actgacaaag
      361 ttttccctaa tcctggtaat tcagacctgg gactaataac tgcttttttc gaagcatact
      421 tggacgctga ctacactgat cggggcctgc tgacaaaaga gtggatgaaa ggaaatcgtg
      481 tttcacgcat cacccgcacg gcttccggtg ctaatgctgg tggtgggaac aaaaccgatc
      541 gcaatccgaa tttagtacac accctcgaca cactggatgt ggagattgca gcagccacac
      601 ttccgatgga ttttaatatt tatgaaattc cgggcagcgt ttatcgtcgc gcaaaagaag
      661 tagtcctgaa aaaagaaagt ccgttcaaag aatggtccgc agcacttcgt gcacccccgg
      721 gtattctgga ctattcccgc gccgctattt ttgcacttat ccgaagcgca caccctgaat
      781 tttatcacta cccgggacgc cttcaggggt atatcaacgc ctatttgacg gaaactgatc
      841 acgagaaccc cagcaaggaa actctcacag ctacccggca tacgccggaa aaagatatcc
      901 tggaagaaat taaccgcgag gtggttactg agcgtgaaac agaagaagaa aaaccacaac
      961 catctgacgc aatggcaggt gaacaggcaa caactgaaac aatggaaccg gatacaactg
     1021 aacattgcca gaacgcgcag tcgctggatg ctcagtcgca ggtgagttcc gctaaccaag
     1081 taaaagtcac cgctgacgaa gtaaacaaaa ttatgcaggc agccaatatc agccagcctg
     1141 acgccgataa gttacttgct gtatcgcgtg gtgaatttgt tgaggggatt agcgacccta
     1201 atgatccgaa atgggtcaag gggatccaga ctcgcgattc tgtgaaccag aaccagcatg
     1261 aatcggaacg gaacgaccaa aaagcggaac aaaacagccc aaatgcgtta caaaacgagc
     1321 cagaaacgaa acaatccgaa ccagtagcgc aacaggaacc ggaaaaagtc tgcaccgcct
     1381 gcggtcagag cggtggcggc aactaccctg attgtggcgc ggtgatggac gacgcaacat
     1441 accagaaaac attcgatgaa gagaatcagg ttgaagttca ggaaaatgat ccggaggaaa
     1501 tggaaggcgc tgaacatcca cacaaggaga atgctggcag cgctcaggac cacgccagcg
     1561 atagtaaaac tggcgagacg gcagatccct taattacggt gaatggtcat cacgaaatca
     1621 catccaccag caggacgtgt gaccatctaa tgatcgacct tgaaaccatg ggaaaaaatc
     1681 ctgatgcccc gatcatctca ataggtgcaa tatttttcga tccgcaaacc ggagatatgg
     1741 gacccgaatt tagtaagact atcgatctgg aaactgctgg cggagtcatt gatcgggaca
     1801 ccattaaatg gtggcttaag caatcacgcg aagcgca
//
LOCUS       DAJTMC010000306         1818 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.313, whole genome shotgun sequence.
ACCESSION   DAJTMC010000306 DAJTMC010000000
VERSION     DAJTMC010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.313"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(317..1465)
                     /locus_tag="OO130_004208"
     CDS             complement(317..1465)
                     /locus_tag="OO130_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5367368.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAALNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITSMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWSIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
BASE COUNT          573 a          465 c          396 g          384 t
ORIGIN      
        1 tggttggcgt agttattaac aacgttgcca aaaccccaga cagtgataaa agacatcagc
       61 gcaatgttgt accatcgcag ctgtttttcc tgaacattcc cagccataga caaatccttc
      121 gccaaaatta taggggcatc tgtaaaacga gggattatgc gcggcaaata agaagtgcgg
      181 aaatagcgaa atgcaaaatt ataacctgct gcacatttta tttttaaacg aataaaattt
      241 ggaataaagc aagtcagata gaagaaacaa aaaagcagag tgtgaaacag taagttcaca
      301 ctctgcttga attcatttac acgtgagcaa cgggtttcgg cgtatgaccg gcgttgcgca
      361 gcagcatcag caaatagata aacgatacgc tggcgatcat gataaacaat aaattatcgg
      421 agaaattctg cattagcata gcggtaaatg acgggccaag cagacttccc acagtatagc
      481 tcaacagtaa ggcctggttc atcgccacca gttgatgatg ttcaactttc tcgcaagccc
      541 atgccatcgc caccggatat agcgtaaagc cagcggcacc gaggatgaat aacgctgggg
      601 ccatcgccgc ctggctaagc atcgcgatac tgccgagaat gacgacaaag acctgaacac
      661 gcaacaccag cagtcgacca aacttatccg ccagacgtcc aatcgaccat tgtccaagga
      721 tacccgcact gaccagtacc gccatccaga aaccaatgct ggcattgctc acccctttgt
      781 gattgaggta cagcggcatc aggccatata gagatcccag aacgattcct gagataatgc
      841 agccattcac gccaagccgc gcctgacgga gttttagcat tgacgtaatt gacgtcgaat
      901 catggttttc agcctgctga ttcagcacac gcgtaaacaa cagcggtaag atccctgcca
      961 acgtcaaacc tgtaacccac ggcaatacgg acatcagctc ggttgaaact ttgctgacca
     1021 gtaactggcc taaaaacgtt cccacgtaat aaaccatcat atacgcagca agcaaacgcc
     1081 cacggttacg tgacgtccca ctgcacatca gcgcgctctc aacaaccacc caaatcatgg
     1141 cacagccgac gcccgcgaca aaacgccaag ccaaccagct ccagaatcca atcatcaggc
     1201 caaggccggc acagccagcg gcaaaaatga aggaggccag ataatagctg cggttaaagc
     1261 caatgcgctt aatgacatac cctgtcagca atgtaccgac aaggttgccg gtaaaatagg
     1321 atgagctgac aacgcctacc tgccatgtgg acatgtgttc ctgggcgagc caaagcggca
     1381 cgagtgtatt taacgccgca atcgccagag tcaacaaaag caggccagac agcaaaagca
     1441 tgacaggccg ggtatacgtg gacatgaatt aaaaccgtga ggaagttcaa atttcatgcg
     1501 catcatgcca ccgccaaaac attgtcaatg agtgaaaata cgcgatatcc cagcggcggt
     1561 attatcgatt tatattactc attcataacg ctaatgctga agagatgatt agtctcatct
     1621 gattgttttt attgaactaa cacaaatttc atacgcttcg ttcagtcgaa aaatttcatg
     1681 gtcacagcaa actcatattt cccgtctatg ctttcagagc aatgcatcgc gtctgatcga
     1741 tttcagacgt accactgact ctcgaaagga gaagaggatg accaaaccgt atgttcgtct
     1801 tgataaaaat gatactgc
//
LOCUS       DAJTMC010000307         1764 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.314, whole genome shotgun sequence.
ACCESSION   DAJTMC010000307 DAJTMC010000000
VERSION     DAJTMC010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1764
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.314"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            <1..278
                     /locus_tag="OO130_004209"
     CDS             <1..278
                     /locus_tag="OO130_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="oxidoreductase, Fe-S subunit; terminal electron
                     transfer protein for the reduction of DMSO; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase subunit B"
                     /protein_id="HCR5367369.1"
                     /translation="QYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDEL
                     RKKHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            280..1143
                     /gene="dmsC"
                     /locus_tag="OO130_004210"
     CDS             280..1143
                     /gene="dmsC"
                     /locus_tag="OO130_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HCR5367370.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLIVTMVLGVIFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVVSVMQGAELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(1178..>1764)
                     /locus_tag="OO130_004211"
     CDS             complement(1178..>1764)
                     /locus_tag="OO130_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR5367371.1"
                     /translation="VLLVDHQAGLLSLVRDIEPDKFKNNVLALGDQAKYFNLPTILTT
                     SFETGPNGPLVPELKAQFPDAPYIAHPGNINAWDNEDFVKAVKATGKKQLIIAGVVTE
                     VCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGAQLMTWFGVACELHR
                     DWHNDIEGLATLFSNHIPDYRNLMTCYDTLTKQK"
BASE COUNT          379 a          424 c          491 g          470 t
ORIGIN      
        1 cgcaatataa cgaaacgaaa ggccatatga ccaaatgcga tggttgttat gaccgtgttg
       61 cggagggtaa aaagccgatc tgtgttgaat cttgtccgct gcgggcgctg gatttcggcc
      121 ctatcgacga gctgcgtaaa aaacatggcg atctggcggc cgttgcgccg ttgccgcgag
      181 ctcactttac caaaccgaat attgtgatca aacccaatgc caatagccgc ccgaccgggg
      241 ataccactgg ctatctggca aacccgaagg aggtgtgaga tgggaagtgg atggcatgaa
      301 tggccgctga tgatcttcac ggtcttcggg caatgtgtag caggtggttt tatcgttctg
      361 gctttggcgc tgctcaaagg cgacctgcga gcagaagccc agcagcgtgt tatcgcctgc
      421 atgtttggtt tatgggtgct gatgggcatt ggctttatcg cctctatgct ccatcttggt
      481 tcaccaatgc gcgcttttaa ctcgctcaac cgggtagggg cttcagcact cagtaacgaa
      541 atcgccagcg gttcgatctt ttttgccgta ggcggcatcg gctggttgct ggcaatgctg
      601 aaaaagctgt caccggcatt gcgtacgctg tggctgatag tgacgatggt tcttggcgtc
      661 atctttgtct ggatgatggt gcgtgtgtat aacagcattg ataccgttcc gacctggtac
      721 agcatctgga cgccgatggg cttcttcctg acgatgttta tgggcggccc gttactgggt
      781 tacctgttgt tgagtctggc tggcgtcgat ggctgggcga tgcgtctgct gccagcaatt
      841 tctgtactgg cactggtagt gagtggcgtg gtgtcagtga tgcagggcgc agagctggcg
      901 actattcata gttctgtgca gcaggccgca gcgctggtgc cggactatgg tgcgctgatg
      961 tcctggcgga tcgtgctttt ggccgttgcc ctgtgcttgt ggattgcacc acagctaaaa
     1021 ggttatcagc ctgcggttcc gctactttct gtatcattca ttctgcttct ggcaggggaa
     1081 ttaatcggtc gcggcgtatt ctatggtttg catatgaccg tggggatggc cgtcgcaagc
     1141 taatcataac aaccggggtt tcggccccgg ttctctttta tttctgcttc gttaacgtgt
     1201 cataacaggt catcaaatta cgataatcag gaatgtggtt agagaacaac gtcgccagtc
     1261 cttcaatatc attatgccag tcgcgatgca gctcgcacgc cacgccgaac catgtcatca
     1321 attgcgcacc agcttgcgac atgcgatccc atgccgaatg ccgggtaatt tcattaaagg
     1381 tccctgacgc gtcagtcacg acaaagacgt caaacccttc ttcaatggct gaaagcgccg
     1441 ggaatgccac gcaaacttcg gttaccacac cggcaataat taactgtttt ttacctgtcg
     1501 ctttgacagc ttttacaaaa tcttcgttat cccaggcgtt aatatttccc gggtgagcaa
     1561 tgtaaggtgc atcggggaat tgtgctttca gttctggaac taatgggccg ttaggaccgg
     1621 tttcaaaact ggtggtaaga atagttggta agttgaaata cttagcctgg tcacccagcg
     1681 ccagcacgtt atttttaaac ttatcgggtt cgatatcccg tacaagggaa agtaaaccag
     1741 cctggtgatc aacaagcaaa acgg
//
LOCUS       DAJTMC010000308         1727 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.315, whole genome shotgun sequence.
ACCESSION   DAJTMC010000308 DAJTMC010000000
VERSION     DAJTMC010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1727
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.315"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            45..323
                     /locus_tag="OO130_004212"
     CDS             45..323
                     /locus_tag="OO130_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005028610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
                     /protein_id="HCR5367372.1"
                     /translation="MSDAVYRRLILMKAMSNITDCSVPDINRMLRFMFGKKRRAYVLN
                     NGGLRMSYIFESALSLAELAIIQSSGALPSPPGVYVSVVLKESRNEGQ"
     gene            310..840
                     /locus_tag="OO130_004213"
                     /pseudo
     CDS             310..840
                     /locus_tag="OO130_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772810.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            1174..1695
                     /locus_tag="OO130_004214"
     CDS             1174..1695
                     /locus_tag="OO130_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5367373.1"
                     /translation="MDVYANSQRVFRFQNGVAIAFKNIQAGTARKFTLSSANNSTKNA
                     AFYLWGNPSRSVVAELGDDSGWHFFSQRNTDNSITFAVNGQVIPLNYGNFDARYKYRT
                     EGVQDVRYGHEMYYSPGSNTVSWRFRAPSGHGLSGIAISDTGRDSADNVNGVYYRPLQ
                     KLINGTWYNVASV"
BASE COUNT          441 a          380 c          478 g          428 t
ORIGIN      
        1 aggcaccgtt ttacaacgga aaatcggata cccggactgt tgacctgtct gatgctgtat
       61 accggcggct gatactgatg aaagccatgt cgaacattac tgactgttcc gttccggata
      121 ttaaccggat gctgagattt atgttcggaa aaaaacggcg agcttatgtt ctgaataatg
      181 gtgggctgag gatgagttac atctttgagt ccgcgctctc gttggcagaa ctggcgatta
      241 tccagtcgtc gggtgcactg ccgtccccgc cgggtgttta tgtttcagta gttttaaagg
      301 agtcccgtaa tgaaggccag tgataaaccc cgccagctgg cggtcccctt tgcgagtacc
      361 ggagataaaa accgtatccc ggacaaggcg acacagcaga ccagagagag cggtaatgct
      421 gcgtatgatt caggttttcc tccggtgacc atgacagcgg tctcagcggg aggtataccg
      481 ccacacggca aggattttaa cggtctgatg tacgatatta ccgcagcaat acggttcgcc
      541 caggctggcg gtttgtacac gtataatgcc ggttttgcgg gggccattgg tggatatgca
      601 aaaggagcca ttctcgccgg agtcgcaaca acagcggtct ggctgaatac cacagacgat
      661 aacctgaccg atcctgaagg ctccgacagt gcgggctggg taaatcttct tgaggatccg
      721 aaaaggatat tcctgcggca gaagaacaat ctgtcagacc ttcagaataa agggacggta
      781 cgggataatc ttcaggttta cagtaaagag cagtcagatc aacgctatgt tcatcgggaa
      841 ggcgataaaa taaccggaga gctgaaaatc cgtggtgtta atgcgctgag gattttcaac
      901 gaagcttttg gcctgatttt tcgtcgttcg gaagagtgcc tgcaccttat tcccaccagt
      961 gaaggtcagg gggaaaatgg cgatattggt cccctgcgcc cgttcaccat taatttgcgg
     1021 acgggtgaaa tatccatgtc gcataaagtg tctgttggcg gtggttcgca ggtcaatggt
     1081 gcgctgggta tcggcgttca gaacgccctg ggggggaatt caattgttct tggtgataat
     1141 gacaccggat ttaaacagaa tggagacggt attctggatg tttatgctaa tagtcagcgt
     1201 gtattccgct ttcagaatgg agtggctatt gcttttaaga atattcaggc cggaactgcc
     1261 agaaaattca cgttatccag cgccaacaac tccacgaaaa atgcagcgtt ttatttgtgg
     1321 ggtaatccat ccaggtctgt tgttgcagag cttggcgatg attcaggctg gcattttttc
     1381 agccagagga atacagataa cagcatcacg ttcgccgtaa acggacaggt aattccgtta
     1441 aattacggaa acttcgatgc ccgctataaa tatcgaacag agggggtaca ggatgtacgg
     1501 tatggccatg aaatgtatta cagccccggc agtaacaccg tttcgtggag atttcgcgca
     1561 ccttcgggac acgggctgtc agggatagcg atatcggata ccggccgtga ctcagcggat
     1621 aacgtcaacg gcgtgtatta ccgcccgctt cagaaactga ttaatggcac ctggtataac
     1681 gtggcgagtg tttaacaatg ttgcatatta aaaaatatta ctgcggg
//
LOCUS       DAJTMC010000309         1704 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.316, whole genome shotgun sequence.
ACCESSION   DAJTMC010000309 DAJTMC010000000
VERSION     DAJTMC010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1704
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.316"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(855..1328)
                     /locus_tag="OO130_004215"
                     /pseudo
     CDS             complement(855..1328)
                     /locus_tag="OO130_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708890.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase
                     domain-containing protein"
     gene            complement(1256..1622)
                     /locus_tag="OO130_004216"
                     /pseudo
     CDS             complement(1256..1622)
                     /locus_tag="OO130_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          379 a          475 c          413 g          437 t
ORIGIN      
        1 tcgtcaaaaa aaatcatctt cgtgaagttt tacgacatag tggaggagtt tgcttctttg
       61 aggtgcaggt tttcgtttgc gtggtttagc tgaacgggcc atataactac ctgaaagaca
      121 atgatattgt ctgtttttat aacggtaatc acaggccatg acaagccgca gccgtcaggc
      181 cgcctactcg gggcggttta gaacgcttat ttagtaccag atcttactta taataaaggg
      241 gcttggcagt ttggataaac tattgtcctt attccactaa tacaaacttt ttgtaaagta
      301 tattaaatat acttcatttc atactgaaat ccccggaagt attcaatcaa acaattacac
      361 cataaaagga agagggggcc ccaattaagt tgtcaagcat gtttactgcg caatcgcttg
      421 gtgctccgtc cctgtacccc gcattggtac tctggcgctc atggggcttc cacttgcgct
      481 tctcccttca catcggggtg acaggtccac gttccccaca agagcctgaa tcgacttcac
      541 gccgctttta tgccggatgc cgcgcaggca gtaaacatat cccacaggaa cgactccacc
      601 attgttttga catcatctat atgctttcga cacatcatca gcggttcact ttcgttcgtc
      661 tcttcgaacc atacctgatc ggctcacgtc cgaccttttc ccatctcgct caccaccaca
      721 tctcttaaat gcagcagcag tggatggttt ggtgcctgcg cctgcacgcc gacaccgagg
      781 ggccttccct catctcttgt gccgcatggc tgcacaggga ctcacgtccc caggacgcgc
      841 cttcgtggca cacagtcatc ggcatagcaa cagtaagcaa cggcaggttt ccattgccag
      901 ttttctttga cagcagtact tcggcctcgc tggatactgt gattccagta ccagcgatcc
      961 tttctggctt ttccactcag gtagcgttta ttcagatact ggtcgaactc attcagtatg
     1021 atgttcgaca gcaacggcga gatcacaccg ccctgtggca tactttcact gaccgcccgg
     1081 aagagaccaa catcaacacg gcgttttcca cagcagtctc ataaaacgtg catcactgat
     1141 cctgcggcag acggctttca tcagcagccg gtgatgtact gtgtcgaagt agctggacag
     1201 gtcaccttca ataacccggc gccccgtgtt tccccgcagt cggtgagctg taatagtacg
     1261 tgatgtctcc cgcccacttc tggttcgggc cactggcgta aaaatcctgc gtcagcagat
     1321 tttctgatac aggcaggccg tgtgcacggt aactgatcgg gctgaacttc tgggaggctt
     1381 ttgcgctcag ttcctgacgg cgcagactgg ctgccacggt ttttacgtta aagtggtaac
     1441 cagagcacac agttcatccg tcaggcgtgg ggcaccgtaa cgctggactg ggtaaaagcc
     1501 ttgcggacaa cgctgtcgca gtgctgacgg aactgctgac gtgtgtttat ccttgtccgc
     1561 cgctgacacc acgtatacca gccgctgcgg gccacccgga gtacgcggca cattgccttg
     1621 atgacggttt acgcggtcgc ctgcggcaac ggctttccgg ctgacggtag tgtggacctt
     1681 tttaccccgg agaacaccgg caag
//
LOCUS       DAJTMC010000310         1667 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.318, whole genome shotgun sequence.
ACCESSION   DAJTMC010000310 DAJTMC010000000
VERSION     DAJTMC010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1667
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.318"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            13..1268
                     /locus_tag="OO130_004217"
                     /pseudo
     CDS             13..1268
                     /locus_tag="OO130_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415521.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YccE family protein"
BASE COUNT          547 a          262 c          342 g          516 t
ORIGIN      
        1 tagacgctat atatgggtag taatatacat ggaattagtt gcactgcaaa taattatttg
       61 aaacaggcct ggaacgatat aaaaaatgag tacgaaaaaa atcaaacata ttcaatcacg
      121 ctttttgaaa acacactggt gtgttttatg cggttataca atgaactcag acgtaaagta
      181 aatgaagagg atactccatg tctggaatgt gaatcactag aaaaagaatt tgaggaaatg
      241 cagaatgata atgatctatc attatttatg agaacattgc gtactaatga tacacaaatt
      301 tattcagggt ttcaggaggt attacatata ctatacaata tgttcaaaat gttgatattg
      361 ttagagtgtc cttgccgggc agaggttcag agtctatcac agattttaaa ggttattatt
      421 ggtatggctt tatggaatat attgaaaaca ttactgcgtg tgatgatgtt ttttctgagt
      481 attgtttaga tgatgaaaat atgagtatcc agccagaaca gataaatacg ccgggaatat
      541 ctgatttaga tactggtatt gatttgtctg gcatatcttt tattcagagt gaaattaata
      601 aaacatatgg attaaaatac gctccagtag atggcgatgg atattgtctg ttaagagcca
      661 tactggtttt aaaagaacat gagtattcat gggcgctggg cggccataag acgcaaaagc
      721 aagtatacga agagtttatt aaaattgttg ataaacaaac gatcgaggct cttgttgata
      781 cggcattcta taatctcagg gaagatgtaa agacgttatt tggcgttaat ctacaatctg
      841 acaacaaaat tcaggggcag ggtggttttt tatcatggag ttttttgtct tttaaaaaag
      901 aatttattga tagttgcttg aatgacaaaa aatgcatact gcatttaccc gaatttatat
      961 ttaatgataa caaggtacgc cttgttttag acaccgaccc agagcagagg gttaatgagg
     1021 tgaaaaattt cctcactgct ctttcagata gcatttgctc attatttatt gttaatagta
     1081 atatggcatc aatctccttg gggaatgaat ccttttcaac agatgacgac cttgagtatg
     1141 gttatttaat aaacactggc aatcattatg atgtttacct ccctcctgaa ctttttgctc
     1201 aggcttataa gttaaaaaat aaggaaatga atgcgcaact cgactattta aatcgttatg
     1261 caatttaatg gcaaaggcat atgctaaaaa ccattgttat tagtctcaca cttttttatt
     1321 ggtaaatatt gtctctgtat tggtaacgcc gaagatattc tgtttagcca caggtgcaat
     1381 tatcagcggc gtacgcgagg caggggctaa tcaggcatag tttgcgtcaa accttgcctg
     1441 tttttgaaga tgtatataga aaaacaggcg ttcaacaagc cattttgcga acctgttccc
     1501 ggaaaaaagt catatttctg tcacactctt tagtgattga taacaaaaga ggtgccagga
     1561 atgaacaaaa cgctaatcgc cgcagctgtg gcagggatag ttttactcgc ttcaaacgct
     1621 caggcacaaa ccgtaccgga aggctatcag ctacagcaag tgctcat
//
LOCUS       DAJTMC010000311         1648 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.319, whole genome shotgun sequence.
ACCESSION   DAJTMC010000311 DAJTMC010000000
VERSION     DAJTMC010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1648
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.319"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(<1..950)
                     /gene="corA"
                     /locus_tag="OO130_004218"
     CDS             complement(<1..950)
                     /gene="corA"
                     /locus_tag="OO130_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HCR5367374.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            1320..>1648
                     /locus_tag="OO130_004219"
     CDS             1320..>1648
                     /locus_tag="OO130_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5367375.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLTLTLLPLFSVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADINSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQ"
BASE COUNT          427 a          427 c          411 g          383 t
ORIGIN      
        1 tacaaccagt tcttccgctt aaagtacaga tacggtgcca ggcccgcgag gatcataaag
       61 ataatcgcgc cagggtagcc gaagctccac ttcagttctg gcataaactc aaagttcatg
      121 ccatagctgg aagcaacgag cgtcggcggc aggaatacca cggataccac cgagaagatt
      181 ttgatgatgc ggttctgctc gatgttgata aaacccatcg ccgcctgcat caggaagttc
      241 accttctgga acagagattc gttatgcggc agcagggatt cgatatctcg caggatttca
      301 cgcgcctgct ccagttgtcc acccggtaag cgtgctttac gtaccaggaa gttgagcgcg
      361 cgctgggtat ccatcagaca caggcgtact ttccagccga tatcttccag ttccgccagc
      421 gtggagagcg cctcatcgta ctcatcgccc tgatgccctt ccataatcac ccggctcaac
      481 tgctccaggt cgctatagat attttcaatt tcatctgcca actgttcgat tttggtttcg
      541 aacagatcca gcagcagctc gtaggcgtta ccgtctacca tcgactggct acgggcacgc
      601 atacgataca gacgaaaagc gggcagttca cgctcacgca gagtaaacag acgaccatca
      661 cggatggtaa atgccacagt ggagttaccg gcgtgatctt ccgcatcttc aaagaagaag
      721 aaggagtgaa tatgcaggcc gtcgtcgtct tcaaagaaac gtgccgatgc ttcgatgtct
      781 tccagttccg ggcgggttgc caggctctgg ccaagttcag attgtacacg cagtcgctcg
      841 tcgtcgtccg gttcgacaag atcaatccat actgcattta caaggggttg tgactcttcg
      901 acttccagcc gggtcagtcg gttattttcc agttgaaatg cgctcagcat gaccgggact
      961 cccaatgctt aaaatatcgg acagttcggt gggcacacag aaacaaattg ggtttcagac
     1021 cattaaacag cctgactcag cgcgacggga aaaattgagg tcgctgacaa ccgctaaggc
     1081 tatcagcaaa aagggatagc cttaggagtt gatcctggat gacaggatag tgagccagta
     1141 tctactgggt gtgtccaagg cgaatgtcct cttagaatga tcgtgcgcgc atgttacgcc
     1201 agcaaaattt tgccgtcaac acgcaacaga acaccacaga acaatagttg aacttcacgc
     1261 tttttggtac tcctcaaggt cgctaacagg ataaaaaaat cccacatcca ggacctataa
     1321 tggcgcatcg gctactcatt ggtaaaggaa tgatcacctt gaatctcaaa cggatttttc
     1381 tcaccctcac cttacttccc ttgttttctg ttgccgctga tgattgcgca ctctcagatc
     1441 cgacgctgac cgtacaggcg tataccgtta atcctcaaac agagcgggtg aaaatgtact
     1501 ggcaaaaagc caatggcgaa gcgtggggaa cgttacatgc tctgctggcg gatattaata
     1561 gtcagggtca ggtgcagatg gcgatgaacg gcggcatcta tgatgaaagc tatgcgccgc
     1621 tcggtttgta catcgaaaac ggtcagca
//
LOCUS       DAJTMC010000312         1621 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.320, whole genome shotgun sequence.
ACCESSION   DAJTMC010000312 DAJTMC010000000
VERSION     DAJTMC010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1621
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.320"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(1..1607)
                     /locus_tag="OO130_004220"
                     /pseudo
     CDS             complement(1..1607)
                     /locus_tag="OO130_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001441428.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
BASE COUNT          420 a          411 c          349 g          441 t
ORIGIN      
        1 caaaaaggca aacagatccg aagcgggata atctttttgg ggagaattgt tccagttagc
       61 aatatatccc gacagggggt tatacacctt agggttcatt tcaaaaggca atagcccttt
      121 ccagtcccat tttcccgtac caggaacggg taatcgcgga tcatggcctg attgacgatc
      181 tggataagca ccagtatgaa cataaccaat attgccgttt acatcagcat agtaccagtt
      241 gatggtcagt gcttgtttcg ctactcctgc caatttttgg ccttcatctg atgagtccag
      301 gccagcaaag acgccacctc tttaccatcc catgcgcggg atttagcgta agccgtttgt
      361 gtcgtctggt cagtttggag aatgttgcca tgcaccgtac gccagacagt aaaggtctct
      421 gcctgaccat ttttcaccgt aatggtttcc tcacggctta acattttcac ccacttacaa
      481 ttatgtaagt agtagcctgg tttctctgcc gacagccgtt cagcaaaaat atcgacatca
      541 tcgccgaaac ctgccgttga tccccaggaa atcacaccat tatgaccaaa aaccagccca
      601 ggataggcaa atggtgtatt gccagtgaca tcataaccag caccgtgcag accaatacca
      661 taagtatacg caggcgcata ccagccaaac tgcggaccat ttaccatgat tgctttcgca
      721 tcctgggctt tgcttttgcc gatcacccac atattgctgg tcgttggata ccccgccaga
      781 ccattggcac caccctgtgc aaattgtgca gcaatagttt cccggttctt ccctgctgtt
      841 aacgccagca gtgcgccatc cgcccctttt gctggtcggt caagcattgg tgcaggtaaa
      901 tcgtagcgtg gcaacagagc tgctgtttgc gagttttgct gattaaattt aagtgggtag
      961 ctactctctt gtacggcaat agtggttggc gctgatgggt ttaccagcca tttcaactga
     1021 ttaaataccg ccatgccttg tgatacacca tatttatctt ttaaagccgt tagcagtgcc
     1081 agattatcaa tttcgctagt gctatcagag aagcggtttg ccatggtgcc cacaaatatc
     1141 atcgcgacat caaacggttc ccagcgctta ggagtaaagc caaatgtatt aaactgtttt
     1201 ggtaagagcg tctctggatt ggtatttacc ttatcaatcc aggcattcat tccatcagcg
     1261 tagccttgca gaatggacat atcctctggg gaaagggcat caatttgcgc ccggatagca
     1321 tccggccagt agttacgacg gatatcttta tcaaatttca caaaatcttt gccaagcact
     1381 tccgcgacag tcccttgagt actgcgacgt gccatttcca tctgaaaaag gcgatcttgt
     1441 gctactacat agccatagcc ataaaatagg tgccatgtat cattggcata aatatgcggc
     1501 atgccgtatt catcgcgaac aatctttatc tcacttgacg actgctcagc cagtgcaggt
     1561 aagctccaat aatacatcag ggaagcagta acacagttca cgatcatacg atttctattt
     1621 t
//
LOCUS       DAJTMC010000313         1612 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.321, whole genome shotgun sequence.
ACCESSION   DAJTMC010000313 DAJTMC010000000
VERSION     DAJTMC010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1612
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.321"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            <1..1566
                     /locus_tag="OO130_004221"
     CDS             <1..1566
                     /locus_tag="OO130_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HCR5367376.1"
                     /translation="IRENMSEFARCHYGYIQIPPVTTFRADGPETPEEEKGYWFHAYQ
                     PEDLCTIHNPMGDLQDFIALVKDAKKFGIDIIPDYTFNFMGIGGSGKNDLDYPSADIR
                     AKISKDIESGIPGYWQGQVLIPFIKDPVTKERKQIHPEDIHLTAKDFEASKDNISKDE
                     WENLHALKEKRLNGMPKTTPKSGQVIMLQNQYVREMRKYGVRGLRYDAAKHSKHEQIE
                     RSIIPPLKNYNERLHNTNLFNPKYQKKAVMNYMEYLVTCQLDEQQMSSLLYERDDLSA
                     IDFSLLMKTIKAFSFGGDLQTLASKPGSTISSIPSERRILININHDFPNNGNLFNDFL
                     FNHQQDEQLAMAYMAALPFSRPLVYWDGQVLKSTTEIKNYDGSTRVGGEAWLNKGCST
                     YQQLYNEFHALYIDKAGIWSAFEGVFATKNVLAFSRGDSVNINHSPHDGLVIINKGNE
                     EVEGTWPNKLQPGIYKNMGSNSVNIIINNTRKIIPPGKVFTLRGGTLNINIPGRSALL
                     LGKTGEPPNYLYL"
BASE COUNT          555 a          304 c          312 g          441 t
ORIGIN      
        1 atcagggaaa atatgtctga gtttgcacgt tgccattatg ggtatataca aatccctcca
       61 gtgacaactt tccgcgccga cggaccagaa actcccgaag aagaaaaagg ttactggttt
      121 catgcttatc aacccgaaga tctttgtacc atccataacc caatgggaga tttgcaggat
      181 tttatcgcat tggttaaaga tgctaaaaaa tttggtatcg atatcattcc tgattataca
      241 tttaacttta tgggaatcgg gggtagtggt aaaaatgacc tggattatcc ctctgctgat
      301 atacgagcga agatcagtaa agatatagaa agcggtatcc ctggctattg gcaaggtcag
      361 gttttgattc cattcattaa agatccagta acaaaagaac gtaaacaaat ccatccagaa
      421 gatatacatc tcactgcaaa agacttcgaa gcaagtaaag ataacatctc taaggatgaa
      481 tgggaaaacc tccatgcatt aaaagaaaag cgtttaaatg gaatgcctaa aacaacaccc
      541 aaaagtggcc aggttattat gttgcaaaat caatacgttc gagaaatgcg aaaatatggc
      601 gtacgaggtt tacgttatga tgcggcaaaa cactcaaaac atgaacaaat agagaggtcg
      661 ataatcccac cgcttaaaaa ttataatgag cggttacaca atactaattt atttaaccca
      721 aaatatcaaa aaaaagccgt tatgaattac atggaatacc tggtaacttg tcagttggat
      781 gaacaacaaa tgtcatcact gctttatgag agagatgatt taagtgccat tgatttttct
      841 ttactcatga aaacgataaa agccttttca tttggtggag atctccaaac ccttgcatca
      901 aaaccgggtt ccacaatctc aagtatccca tcagaaagac ggatattgat taacattaac
      961 cacgattttc ctaacaatgg taatcttttc aatgactttc tatttaacca tcaacaagat
     1021 gaacaattag caatggcata tatggccgct ctcccgttca gcagaccttt agtttactgg
     1081 gatggccaag tattaaaatc aacgactgaa attaaaaatt atgatgggtc cacgcgtgtc
     1141 ggcggtgagg cgtggcttaa taaaggttgc tctacctatc agcagctcta caatgaattc
     1201 cacgcattat atatagataa agcaggaata tggagcgcat ttgagggtgt atttgcaact
     1261 aaaaacgttc tggcctttag tcgtggggat tctgtgaaca ttaatcactc tcctcatgat
     1321 gggctagtta taataaataa aggaaacgaa gaagttgaag gtacctggcc taacaaattg
     1381 caacctggaa tatacaaaaa catggggagt aatagcgtta acattattat taataatacc
     1441 cgaaaaatta tcccccctgg taaagtattt acgcttagag gcggaactct aaatatcaat
     1501 attcctgggc gtagcgctct tcttttaggg aaaacaggag aaccgccgaa ctatctctat
     1561 ttataattta atgttatatc tgccccgata aaacggggca gataatatgt tt
//
LOCUS       DAJTMC010000314         1589 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.322, whole genome shotgun sequence.
ACCESSION   DAJTMC010000314 DAJTMC010000000
VERSION     DAJTMC010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1589
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.322"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(149..430)
                     /locus_tag="OO130_004222"
     CDS             complement(149..430)
                     /locus_tag="OO130_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HCR5367377.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
     gene            860..>1589
                     /gene="pdeC"
                     /locus_tag="OO130_004223"
     CDS             860..>1589
                     /gene="pdeC"
                     /locus_tag="OO130_004223"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HCR5367378.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIAPVNGYTIAPADYKREPNVSIYYYRDTPFSSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSKEASAATFSPLIHLKDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLITHFYNHL"
BASE COUNT          431 a          359 c          349 g          450 t
ORIGIN      
        1 cgtaacggga taccagatca agagcctgtc caatgtgcat aaaaaaatcc ggaaacaagt
       61 gagcgtttcc ggattcttac acagccactg gatcggtcaa ctgctcctta actgatcggc
      121 attactccgt tatggaggct tctacgtatc aggtcaaaat cattggccat tgtggcggtg
      181 tctgattcat ctcttctatc atcaccgact cgatgttgtg ccagatagcg gtgatgtccg
      241 ccattgtgat gccaagcaac cccattgcga accaacaggc ggaaacaacg cccagaccgc
      301 tgctgatcac cgcaagcccg acataatcag accgacgaaa acggatgttg agtgtgctgg
      361 ccagaaacat cattacggca ctaagaagtt gccagcgaag aagaaccacg ccagtggtga
      421 gggtagccat caaacaattc ctctgaaaag agccgatgcc ctggacagcg cggcgttgtt
      481 cacgggaggc gtggtacact ctggctatcg cggggcttgc agaacacaaa aatgaaacac
      541 tctgtttgtt tcattaattt tgtgaaatat atcacaattg attgtttgtt agccatatta
      601 ggccgtgact tttattgctg tacagattat gtggtttttc agtggcatta agggcatatc
      661 ttcccgccgc ctctgcattc ctgtaggaaa ttaattttga atatcaatga attattttca
      721 tccaggtgac gattagaaag gtatcaattt caaatcaggc aaaagtgcta tttataccgt
      781 aagatttatc taaagacgtc ggtacccagg gttttcacct tacaatggcc gggtataaac
      841 aggcaggaaa ttgatagcaa tgagtcatcg tgcacgacac caattactgg cgttgccggg
      901 cattatcttt ttagttctct ttcccatcat tctttcgcta tggattgcct tcctttgggc
      961 aaaatcagaa gtgaataatc agctccgaac ctttgctcaa ctggcactgg ataaatccga
     1021 gctggtcatt cgccaggcag atttagtgag cgatgcagct gaacgctatc aggggcaagt
     1081 ttgcactcca gcccatcaaa agcgaatgtt gaatattatt cgtggctatc tttatattaa
     1141 tgaattgatc tatgcccgtg ataaccattt tttatgctca tcgctgatag cgcctgtaaa
     1201 cggctatacg attgcaccgg ccgattataa gcgtgaacct aacgtttcta tctattatta
     1261 ccgcgatacg cctttttcct ctggctataa aatgacctat atgcagcggg gaaattatgt
     1321 ggcggttatc aaccctctct tctggagtga agtgatgtct gatgatccga cattgcaatg
     1381 gggtgtgtat gatacggtga cgaaaacctt tttctcgtta agcaaagagg cctcggcagc
     1441 aacgttttct ccgctgattc atttgaagga tttaaccgta caaagaaatg gctatttata
     1501 tgcgacagtt tattcgacaa aacgcccaat tgcagccatt gttgcgactt catatcaacg
     1561 tcttataacc catttttata atcatctta
//
LOCUS       DAJTMC010000315         1589 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.323, whole genome shotgun sequence.
ACCESSION   DAJTMC010000315 DAJTMC010000000
VERSION     DAJTMC010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1589
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.323"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            <1..597
                     /gene="argO"
                     /locus_tag="OO130_004224"
     CDS             <1..597
                     /gene="argO"
                     /locus_tag="OO130_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HCR5367379.1"
                     /translation="AMILPLGPQNAFVMNQGIRRQYHIMIALLCAISDLVLICAGIFG
                     GSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAEVLKQGRWKIIATM
                     LAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWFFGLAILAAWLAPR
                     LRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            690..1430
                     /locus_tag="OO130_004225"
     CDS             690..1430
                     /locus_tag="OO130_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HCR5367380.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
BASE COUNT          375 a          373 c          437 g          404 t
ORIGIN      
        1 gctatgattc tgccgctcgg cccacaaaat gcttttgtga tgaatcaggg cattcgtcgt
       61 cagtaccaca ttatgattgc cttactttgc gctatcagcg atttggtcct gatttgcgcg
      121 gggatttttg gtggtagcgc gttattgatg cagtcgccgt ggttgctggc gctggtcacc
      181 tggggcggcg tagtcttctt gctctggtat ggttttggcg cttttaaaac agcgatgagt
      241 agtaatattg agttagctag tgccgaagtc ctgaagcaag gcagatggaa aattatcgcc
      301 accatgttgg cagtgacctg gctgaatccg catgtttacc tggatacttt tgttgtactg
      361 ggcagccttg gcgggcagct tgacgtcgaa ccaaaacgtt ggtttgcgct cgggacaatt
      421 agcgcctctt tcctgtggtt ctttggtctg gctattctcg cagcctggct ggcaccgcgc
      481 ctgcgcacgg caaaatcaca gcgcattatc aatctggtag tgggatgtgt catgtggttt
      541 atagccttgc agctggcgag agacggtatt gctcatgcac aagccttgtt cagttaggcg
      601 ctatctgatg gaaaaataaa acagaggcgc taagcttgcc tccagaggtc ctgaattttg
      661 ggcaagtcaa ttaacacgga gagactaacg tgaagttcaa agttatcgcc ctagcggcat
      721 taatgggtat tagcgggatg gcagcgcagg ctaacgaatt gccggatgga ccgcatattg
      781 tcacctccgg tacggcaagt gtggatgcgg tgccagacat tgccactctt gcgattgaag
      841 ttaacgtggc cgcgaaggat gccgctacag ccaagaaaca ggcagatgag cgcgtcgcac
      901 aatacatttc cttccttgaa ctcaatcaga tcgcgaaaaa agatatcagc tcagcgaatt
      961 tacgcaccca gccagattat gattatcagg atggtaaaag tatcctgaaa ggctaccgcg
     1021 ctgtgagaac ggtggaagtc acgctccgtc agttagacaa actgaattcc ttgctggatg
     1081 gcgcgctgaa ggcgggtctt aacgaaattc gttcagtgtc gctgggcgtg gcgcagccgg
     1141 atgcctataa agacaaagcg cgtaaggcag cgattgataa cgcgattcat caggcgcagg
     1201 aactggcgaa cggctttcat cgtaaactgg ggccggtata tagcgtgcgc taccatgttt
     1261 ccaactatca gcccagccca atggtgcgga tgatgaaagc cgatgccgcg ccggtgtctg
     1321 cccaggaaac ttacgagcag gccgctattc agtttgatga tcaggtcgat gtggtcttcc
     1381 agttagaacc tgtggatcaa caacccgcta aaacacctgc agcacaataa tacttgtagc
     1441 ccgacgtatt cgtcgggcta atgcctttac ccgatattgc ctcctttttc tgtaattttc
     1501 tgtaaaacca tgacgtaatc acctatcttt gctcgcggtt tcatcaagga taggctgact
     1561 gaaacggtta agcggacaag gatgcgggg
//
LOCUS       DAJTMC010000316         1584 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.324, whole genome shotgun sequence.
ACCESSION   DAJTMC010000316 DAJTMC010000000
VERSION     DAJTMC010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1584
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.324"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(70..492)
                     /locus_tag="OO130_004226"
     CDS             complement(70..492)
                     /locus_tag="OO130_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5367381.1"
                     /translation="MKIRHEHIESVLLALAAEKGQAWVANAITEEYLRQGGGELPLVP
                     GKDWNNQQNIYHRWLKGETKAQREKIQKLIPAVLAILPRELRHRLCIFDTLERRALLA
                     AQEALSTAIDAHDDAVQAVYRKAHFSGGGSSDDSVVVH"
     gene            complement(476..703)
                     /locus_tag="OO130_004227"
     CDS             complement(476..703)
                     /locus_tag="OO130_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR5367382.1"
                     /translation="MLKIDAIAFFGSKTKLANAAGVRLASIAAWGELVPEGRAMRLQE
                     ASGGELQYDPKVYDEYRKAKRPGKVIHENQA"
     gene            780..1187
                     /locus_tag="OO130_004228"
     CDS             780..1187
                     /locus_tag="OO130_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5367383.1"
                     /translation="MKNETFGARLLHRRKKLKLSQAALGKLVKVAHVTISQWERDETQ
                     PAGKRLFALSQALQCSPTWLLFGDEDKQPGEPIPDNQPAILTEDQKELLQLFDALPES
                     EQKALLSEMRARVENFNKLFEELLKARKRSANK"
     gene            1391..1543
                     /locus_tag="OO130_004229"
     CDS             1391..1543
                     /locus_tag="OO130_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HCR5367384.1"
                     /translation="MDTIDLGNNESLVCGVFPNQDGTFTAMTYTKSKTFKTEAGARRW
                     LAKNTS"
BASE COUNT          446 a          414 c          326 g          398 t
ORIGIN      
        1 ccgaaccttc ctccggatat aatctgtgat tccccaatca acagaaccag aggaggttcg
       61 gcataaacat caatgcacaa cgacagaatc gtcggacgac ccaccaccgc tgaaatgtgc
      121 tttccggtaa acggcctgaa ctgcgtcatc atgcgcatca attgccgtac tcaacgcttc
      181 ctgcgccgcc agtaatgcac ggcgttccag ggtatcgaag atacagagtc ggtgacgcag
      241 ctcacgcgga agaattgcca gaaccgcagg gatcagtttc tgaattttct ccctttgcgc
      301 tttcgtttca cctttcaacc aacggtgata gatattctgc tgattgttcc agtccttgcc
      361 tggtaccaga ggcaattcgc cgcccccctg ccgcagatat tcttcagtaa ttgcgttagc
      421 gacccacgcc tgcccttttt cggctgccag ggctaacagc actgattcga tgtgctcatg
      481 cctgattttc atgaatcacc ttcccaggtc gttttgcctt acgatattcg tcataaactt
      541 tggggtcgta ctgaagttcc ccgccagatg cctcttgcag gcgcatcgcg cgaccttcag
      601 gaaccagttc cccccatgca gcaatgcttg ccagcctaac tcctgcggca ttggcaagct
      661 ttgttttgct gccaaaaaac gctatagcat caattttcaa catatcgagc tccttagatt
      721 ttcctaagga aactagatcg tagagaaacc taagtcaaga aagattagaa ttccctaata
      781 tgaaaaacga aaccttcggt gctcgcctct tacataggcg taaaaaatta aaactgtctc
      841 aggccgcatt aggtaagctg gtcaaagtgg ctcacgtaac aatttctcaa tgggaaagag
      901 atgaaacaca gccggcgggg aaaagattat tcgcactgag ccaagcgctt cagtgctcac
      961 cgacttggct tctttttggg gatgaagata aacaaccagg cgaaccgatc ccggataatc
     1021 agccagccat tctgacagaa gatcaaaaag agttacttca actgttcgac gcactgcctg
     1081 agtcagagca aaaggccctg ttgtcagaga tgcgtgctcg agttgaaaat ttcaacaaac
     1141 tttttgaaga actactcaaa gctcgcaaaa gaagcgcaaa caaataatcc ccttttttct
     1201 ccacaccctg taataaaaag cacaaacttt caaacacttg tgttttttac atcaaaaaaa
     1261 cttaggtttt ttatacataa aatcttgact ccacgtctta ggttattcta aatttcattc
     1321 atcaagacac cgcacggtgt tctcagcaaa cagttccact accccggcgt taaggggaaa
     1381 tgaggtcagc atggatacta tcgatcttgg caacaacgaa tccctagtgt gtggcgtgtt
     1441 tcccaatcag gacggcacat tcaccgccat gacgtatacc aaaagcaaaa cgtttaaaac
     1501 cgaagctggc gcacgtcgct ggcttgccaa aaataccagc taatccattt attggattaa
     1561 ttcaatattc tcgctgtaga ggta
//
LOCUS       DAJTMC010000317         1574 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.326, whole genome shotgun sequence.
ACCESSION   DAJTMC010000317 DAJTMC010000000
VERSION     DAJTMC010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.326"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            <1..91
                     /locus_tag="OO130_004230"
     CDS             <1..91
                     /locus_tag="OO130_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HCR5367385.1"
                     /translation="GIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            94..168
                     /gene="yoaJ"
                     /locus_tag="OO130_004231"
     CDS             94..168
                     /gene="yoaJ"
                     /locus_tag="OO130_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HCR5367386.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(227..475)
                     /locus_tag="OO130_004232"
     CDS             complement(227..475)
                     /locus_tag="OO130_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCR5367387.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(623..805)
                     /locus_tag="OO130_004233"
     CDS             complement(623..805)
                     /locus_tag="OO130_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HCR5367388.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYDTENEHDVCGW"
     gene            complement(809..1169)
                     /gene="yeaR"
                     /locus_tag="OO130_004234"
                     /pseudo
     CDS             complement(809..1169)
                     /gene="yeaR"
                     /locus_tag="OO130_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein"
     gene            complement(1342..>1574)
                     /locus_tag="OO130_004235"
     CDS             complement(1342..>1574)
                     /locus_tag="OO130_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HCR5367389.1"
                     /translation="FFVQFIDVNAPHTGISFFILATTLELVSFCYLSFLIISGAFVTQ
                     YIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
BASE COUNT          432 a          375 c          350 g          417 t
ORIGIN      
        1 cggtattatt tttccggttg taatctttat tacagcggtc gtatttttag catggttttt
       61 tattggcggc tatgctgccc cgggggcata aagatgaaaa aaacaacgat tattatgatg
      121 ggtgtggcga ttattgtcgt actcggcact gagctgggat ggtggtaacg tcacctctaa
      181 aaaatagcaa aggctgcctg tgtgcagcct ttgtgcaatt taagcgttaa cttttaatct
      241 tcctgtagat aaatagcacg acaatcgcac caataacggc aaccacgaag ctgccaaaat
      301 tgaagccatc gactttacca aagccaaaca gcgtgctgat ccatccgccg actacggcac
      361 cgactatccc cagcaggata gtcataaaga atccaccacc atctttacct ggcatgatcc
      421 acttcgccag aataccggca ataagcccaa aaataatcca tgacagaatg cccattgttt
      481 cctcacttat ctgttttgca ttagcgggtt agtcgctgat aaaaagcata gcacaacatc
      541 gggagggcaa gatttgtgac gagcatcacg gaggattttt ttgcgatggc gcagaaattg
      601 cgccatcaac gatcagtgat aattaccaac cacaaacatc atgttcgttt tccgtgtcat
      661 aagaacgcac ggtattcacc agatctttta tcacttcagc cgccacttct ggcaccagca
      721 aagtcatcgg cgtctctgtt tcataatcga cagaaacgcc attactgtta ttggtgacgg
      781 tcacggtata cgttgctttg cccatgattc atttcccgtt atgaatgact ttccgttgtt
      841 gcgcaccttc catcaggact tcaggagcca cgaagaagtc aatgttgaca taagtatcgt
      901 cagtcatggc ttcaatgttg tgccactttt ctggagggaa caccgcaaac tgccccgctt
      961 cgataaggat cacctgatca ggctctgcac tgtgttcatc agcgtagccg agatatttga
     1021 ccgccccatg cataacggaa aggcgtgggt aacccccctg gcgcgttcct ttatcaagat
     1081 gacgttcgaa ttattccggc aggcgcagtt tgtttattcc agaaaggcgt tgagcgcgta
     1141 tgaatataat tctgtgggat ttgcagcatc cttttccctc cttcggtgaa tgcgctgaaa
     1201 acggtttatt ccagccgttt cagggtacgc ctgataattt gcattttaaa taccatttat
     1261 tggttacttt ttagcaccat atcagagaaa aatcagggag gattacaagt gggaagcccg
     1321 cgacaacgcg ggctgaatgt atcaggattg cagcgtcgcc agtcgggcgg cgaaacccac
     1381 gaacatcaaa ccaatcagtg agttgccaac tttagccagt ttctttttgg tacgtatgta
     1441 ctgcgtgaca aaagcaccag aaataatcag gaagctcaaa tagcagaaac tcaccagttc
     1501 cagcgtcgtc gccagaataa agaatgaaat tcccgtatgt ggggcattaa catcgataaa
     1561 ctgtacgaaa aacg
//
LOCUS       DAJTMC010000318         1556 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.328, whole genome shotgun sequence.
ACCESSION   DAJTMC010000318 DAJTMC010000000
VERSION     DAJTMC010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.328"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            <1..627
                     /locus_tag="OO130_004236"
     CDS             <1..627
                     /locus_tag="OO130_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HCR5367390.1"
                     /translation="DQKSVNVRLENKGNNPLLVQSWLDTGDDNAEPGSITVPFTATPP
                     VSRIDAKRGQTIKLMYTASTSLPKDRESVFWFNVLEVPPKPDAEKVANQSLLQLAFRT
                     RIKLFYRPDGLKGNPSEAPLALKWFWSGSEGKASLRVTNPTPYYVSFSSGDLEASGKR
                     YPIDVKMIAPFSDEVMKVTGLNGKASSAKVHFYAINDFGGTIEGNASL"
     gene            662..1556
                     /locus_tag="OO130_004237"
                     /pseudo
     CDS             662..1556
                     /locus_tag="OO130_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
BASE COUNT          455 a          338 c          340 g          423 t
ORIGIN      
        1 gatcaaaaaa gtgtcaacgt acgtctggaa aataaaggga ataacccgtt gcttgtccag
       61 agttggttag atactggcga tgacaacgct gagcctggca gtattacagt cccttttact
      121 gccacgccgc cagtatcgcg tattgatgcc aaacgtgggc aaacaatcaa attaatgtac
      181 acagccagca cctcactgcc taaagacaga gagagcgtgt tctggtttaa cgtactggaa
      241 gttccaccaa aaccagatgc agaaaaggtc gcgaatcaaa gtctgctgca actggcattt
      301 cgcacacgta taaaactttt ctatcgcccg gatggattga agggaaatcc ctctgaagcc
      361 ccgttagccc tgaagtggtt ctggtcaggt tcagaaggta aggcgtcatt gcgagtgacc
      421 aatccaaccc cttactacgt ctcttttagc agtggtgatt tagaagctag cggtaaacgc
      481 tatccgattg atgtgaaaat gattgcacca tttagtgatg aggtcatgaa agtcactggc
      541 cttaatggca aagcaagttc tgcaaaagta catttttacg ccattaatga ttttggtggc
      601 accattgaag gtaatgccag tctgtaatca ggcaaggata taattccgca ggaagcataa
      661 cgtgactata aaaccaacta aaaattatca taatcatctg acccgtatcg ctacgttttg
      721 cgcgctgctg tattgcaata gtgctttctg tgctgaactt gttgaatatg accatacctt
      781 cctgatgggg cagaatgcct caaatattga tctcagccgg tatagtgaag gtaaccctgc
      841 tataccgggt atgtacgacg tcagtgttta tgtcaacgat caacctatta ttaaccaaag
      901 cattacattt attgaaattg aaggaaaaaa aacgcacaag cctgtatcac acttaagaat
      961 ttattgcagt ttcatataaa ttctcccgat ataaataacg aaaaagccgt tctgcttgcc
     1021 agggatgaaa cactcggcaa ttgccttaat ttgacggaaa ttatccctca ggcttctgtt
     1081 cgttatgacg ttaacgagca acgtctggat atagacgttc ctcaggcatg ggtaatgaaa
     1141 aattaccaaa actatgttga tccatcgtta tgggaaaatg gcattaatgc ggccatgttg
     1201 tcatacaacc tcaacggata tcatagtgaa actcctggtc gaagaaatga cagcatttat
     1261 gctgctttta acggtgggat gaatttaggt gcatggcgac tgcgtgcctc aggcaactac
     1321 aactggatga ccgattctgg cagtaattat gattttaaga atcggtatat acagcgtgat
     1381 atcgcctcgc tgcgttctca actcattctt ggtgagtctt atacaacagg cgaaaccttt
     1441 gattctgtca gtatccgcgg cattcgttta tacagcgaca gccgcatgtt gcctccgaca
     1501 ttagcgagtt ttgcgcctat cattcatggc gttgctaata ccaacgccaa agtcac
//
LOCUS       DAJTMC010000319         1546 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.329, whole genome shotgun sequence.
ACCESSION   DAJTMC010000319 DAJTMC010000000
VERSION     DAJTMC010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1546
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.329"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(918..1394)
                     /locus_tag="OO130_004238"
     CDS             complement(918..1394)
                     /locus_tag="OO130_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000469298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5367391.1"
                     /translation="MFIDSEKRLKQLSDEAKKNTEDLEEAKKNSRFTQVSPKGWERVR
                     ELLKDSQGISALKLYSFLAEHIDPTCGAVVADQQFLAEKLGVSRSTIIRWLNYLESKN
                     ALVRIPVAGKVCAYALDPHEVWKGYNTTKNHAAFVTKTLVNKDGDIQRRIMAMFSN"
BASE COUNT          353 a          412 c          383 g          398 t
ORIGIN      
        1 aaaaaaacaa caacttacaa atcggcgtaa aagatctatt ctaggaaagg gtgttgcgcg
       61 caacgcaacg cccttttggg gtgtcggggc gaagccctga cccagtcacg tagcgatagc
      121 ggagtgtata ctggcttact cctctgcaag cctcgtcagc catgattttg cccgatttcg
      181 cgctctccac gttcaacagc tcgatcatgt ttttgccgtc cgttcaccgt taaaacgcct
      241 cagaaatcgg gacaggatgt gggaatgtcg gaccgcgcac gcgcactcag acattcccca
      301 cctggtcagg gcttcacccc gacaccctgc atccgtgcgt aagtgctcaa tgtaacttcc
      361 ggttctcttc cgcttcctcg tcgattcgct cgctacgctc ggtcatcgac ttgcggcgag
      421 cgggatcacc tctcgcatgt tgtcggtgat cccgttgttg ttctttcggg gatgagtcgt
      481 cagatagccc ttcaaacacg ctcagaagct ctcagacgca cgaaacgggt ttaaatgccc
      541 gccagggcat ggaaggcgcg aaaaacgctt acagaggctc tcaggaggtc aggcgcgccg
      601 atacccctaa ccaggggctt tcaggtcgct aggaaccgcg tcagctccct gcaaagccta
      661 tttgcacggt acaggcagct attcggctgg ttggtggtcg tagaccgtcg gtgttacagc
      721 gatgggggtc tggggttctc cccagacggt ttccggttgc gctcaaggcg ctgccggaac
      781 cgtggcgacg cggtcgttct tgggatcggg gagcaaaaac acgcaaggga ttttgaggcg
      841 gcaaaagagc aatgcaggat agacaatcat ggtcaaaacg ttaacacgta gcccctgatt
      901 gtccgcctgc cgctggctca atttgaaaac atggccatga ttcggcgctg aatatcaccg
      961 tctttgttga ccagtgtttt agtgacaaac gctgcatggt ttttcgtagt gttgtatccc
     1021 ttccagactt catgtggatc gagggcatac gcacaaacct taccagcaac ggggattcta
     1081 actaatgcat tttttgattc taagtaattg agccaccgaa taattgtgct tctgctaact
     1141 ccaagttttt cagctaggaa ttgctgatcc gcaacgacag cgccacacgt aggatcgata
     1201 tgctccgcta aaaatgagta cagcttcagt gctgatatgc cttggctatc cttcagcagc
     1261 tctcgaacgc gttcccaacc ttttggggat acctgtgtaa accttgaatt cttctttgct
     1321 tcttcgagat cctcggtgtt tttctttgcc tcatctgaaa gttgtttcag tcgtttttct
     1381 gaatcaataa acatgatttt ttccctctga aaatcccatg cactaacgtt ggttcaaatc
     1441 taagccatca tgacacatct gtgcaacacc ttgttgcgtg ttgtcgcatc caggatgcga
     1501 caacgttgtg tcaccctgga tgcgacaggt aaacaaaaaa cacact
//
LOCUS       DAJTMC010000320         1542 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.330, whole genome shotgun sequence.
ACCESSION   DAJTMC010000320 DAJTMC010000000
VERSION     DAJTMC010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1542
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.330"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(<1..186)
                     /locus_tag="OO130_004239"
     CDS             complement(<1..186)
                     /locus_tag="OO130_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HCR5367392.1"
                     /translation="MKSMDKISTGIAYGTSAGSAGYWFLQWLDQVSPSQWAAIGVLGS
                     LLLGLLTYLTNLYFKIRE"
     gene            complement(479..553)
                     /locus_tag="OO130_004240"
     tRNA            complement(479..553)
                     /locus_tag="OO130_004240"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(518..520),aa:Gly,seq:tcc)
     gene            complement(557..632)
                     /locus_tag="OO130_004241"
     tRNA            complement(557..632)
                     /locus_tag="OO130_004241"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(597..599),aa:Thr,seq:tgt)
     gene            complement(635..711)
                     /locus_tag="OO130_004242"
     tRNA            complement(635..711)
                     /locus_tag="OO130_004242"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(675..677),aa:Arg,seq:tct)
     gene            complement(713..789)
                     /locus_tag="OO130_004243"
     tRNA            complement(713..789)
                     /locus_tag="OO130_004243"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(753..755),aa:Ile,seq:cat)
     gene            complement(979..1533)
                     /locus_tag="OO130_004244"
     CDS             complement(979..1533)
                     /locus_tag="OO130_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HCR5367393.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYIGGGKTGN
                     MFNQLLASKKLTKTAINEALRRMKKAGLNKSELEAFLRDMINGKQKSWLAHCTDAEAL
                     CIDRVISEVLAEHPGLISVLRQRYEGRGMTKRKMAELLNDAHPKWSLRTCERRIEHWL
                     KVAEFILYKPMVMAFGIEKKVIAF"
BASE COUNT          418 a          413 c          325 g          386 t
ORIGIN      
        1 ttctcttatt ttgaaataca gattcgtcag ataagtcaga agccccagaa gcagacttcc
       61 cagcacacca atcgcagccc actgtgatgg actgacctga tccaaccact gcaaaaacca
      121 gtagccggca ctgccggcgg aggtgccgta ggcaatgccc gttgaaattt tgtccatgga
      181 tttcatagcc tcacctccgc aaataacgga tggcgtagtt ttacactgag aaatgaaagg
      241 gatttgaaaa gaaaaaaacg caaaagcggg cgaaacgata tatacagtaa ggaaagcact
      301 ctatccaaca aaccacccac agttaatcgg aataaaagca gagtgcttat gaatgatcgc
      361 acgcccgaag gttagtattt ctgcacagca attttgcaaa aaaaggcgat cattcataac
      421 ttaaacgtct ttcagtcact ccgggatttc ccatcatcgc agactgaaag actctgactg
      481 gagcgggcag cgggaatcga acccgcatca tcagcttgga aggctgaggt aatagccatt
      541 atacgatgcc cgcatatggt gccgactacc ggaatcgaac tggtgaccta ctgattacaa
      601 gtcagttgct ctgcctgctg agctaagtcg gcgctggccc accaccgagg actcgaacct
      661 cgcaccgtca acttagaagg ttgatgctct atccggatga gctagtggtg gttggtggcc
      721 cttgctggac ttgaaccagc gacctggcga ttatgagtcg ctcgctctga ccaactgagc
      781 taaagggccg gaggcagaat aataaccata tgtcatcaca tctgcaaact catctgacca
      841 ccagcacgtt taacgtcctg tgccgttttt caggtataaa aaaacccgca taaagcgggc
      901 tctttcaaat gtccatgtct gctattcgcc tcgcggtaca gctttgcgaa gcgtacaaga
      961 attgaagcag tttttacgtc aaaaagcaat aacttttttc tctataccaa aagccataac
     1021 cattggtttg tacaaaataa attctgccac ctttagccaa tgctcaatgc gtctttcaca
     1081 ggttcttaaa ctccatttcg ggtgtgcatc attcagcagt tcagccattt tgcgtttggt
     1141 catcccccgc ccctcatacc gttgccgaag gacgctaatc aatcctggat gctctgccag
     1201 cacctcactt atgacccgat caatacataa cgcctctgca tcagtacaat gcgccagcca
     1261 gctcttttgc ttaccgttaa tcatatcccg caaaaaagcc tcaagttcag acttgttcag
     1321 acctgctttt ttcatcctcc ggagcgcctc gttaattgcc gtttttgtca gctttttaga
     1381 ggccaacaac tggttgaaca tattccccgt cttaccgccg ccaatatacg accagcgccc
     1441 ccacatgcgc agttttccct gaatccagac actttccagc gtggtgagac gaaggtgttc
     1501 cccgcttttg cctgtatttg ttgggtaaat cataaataac ct
//
LOCUS       DAJTMC010000321         1522 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.331, whole genome shotgun sequence.
ACCESSION   DAJTMC010000321 DAJTMC010000000
VERSION     DAJTMC010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1522
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.331"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(1..1386)
                     /locus_tag="OO130_004245"
                     /pseudo
     CDS             complement(1..1386)
                     /locus_tag="OO130_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
BASE COUNT          521 a          264 c          224 g          513 t
ORIGIN      
        1 agcgctggga gcttaccacc ataacaggcc catcatcgac agaggagaac aggataaata
       61 tattatcgtt tcgtgttaat ttattagtgt taaaggttct taacttttct tcagtttcta
      121 taaatatagg tgaaacatca tcactacgaa tccactcttt ataaagatac caggcatttg
      181 ctttgatcat atcatccgtt gatcgtaagg cctgatcgat aagttggata aaaaaattgt
      241 tatttttatc aatcagccct ttattcatat taaacctgtt aataaaatgg atcatctgtt
      301 gggtatcgaa atccaccttg ctgaatcgat gttgacgcat aaaatcatcg aacttgttat
      361 cttcaccaga atggatataa ctgcgtataa ttggatcttt tatataagga caatggctaa
      421 atgttttctt catattctca ataaagtttt tattaacaat attaagaatt tttaattgca
      481 taaccagttt tactgccaga acggatttca atatcattgc attatctggc aatgtatcaa
      541 tcatttttat aatgttattg atataaccat cgagatcttc attcccaaat atatctgaat
      601 aatcaatagt taattccaat tgttctgtaa tgcaatcttt aaaattccct gtcgacattc
      661 gcacatgatc aaacataaca gctgtcaaat tcgatttaat aaatgatatt tctttggcag
      721 acagaaactc tgctctggtg aagtgcgaag cgtaaagata acaattatcg aagtttgaat
      781 ttctaatctg acattcgcga aaattactat gaccaagttg actctgcgag aagtcaactg
      841 tatcaagaat atcttggtta aaatcaacac attcgccact cgttttgaga aaaatcacat
      901 tgtgtaaacg tcgacattta aatatgacat tatcaactgt tgccttagta aaattgcaat
      961 tctcaatttc tttacattcg aaagttacat gttgtagatt tgatgataaa aaagacacct
     1021 tagaaataac gtcaccttta aaatgacaat ttgttacatc aaaatcagac aggtcgatat
     1081 acccctgcga atctttatcc atcagaaaat tacaagcgca ttctattttt tcatttctgt
     1141 agaattttac acttatagta tcattactaa ttgagacttc acgtaacata tttattgcat
     1201 gattttttcg ttccgttgtt ttatatttaa aaatatatta ctgatataaa taaagttatc
     1261 tctgtttaaa cctgtcaccc cattgaaatt acgtagtaaa tcaagatcta ctatattatt
     1321 tccttgaacc acatcatgtt tcatcaaaga gttattgcca ccagaatttg tgatatgacc
     1381 tatcataaaa cctctgtaat acagaaataa aataacctgg aaaagtagcc tgagcgtcca
     1441 gacactaccg gcgaaaggaa aagaatctct gataaggcat tgagataatg gacattctta
     1501 gtgaagaaga agtgaagtac ta
//
LOCUS       DAJTMC010000322         1512 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.332, whole genome shotgun sequence.
ACCESSION   DAJTMC010000322 DAJTMC010000000
VERSION     DAJTMC010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1512
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.332"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            <1..1301
                     /locus_tag="OO130_004246"
     CDS             <1..1301
                     /locus_tag="OO130_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001466140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HCR5367394.1"
                     /translation="GLDIAELQSQFAGFGASAKESMGIQGSEELFRNMIGYSRLMGRS
                     EEEIKRALTALSQMAGKGQIMAEELKGQLAEAVPGMVQVFAKATGKSEQELFDAMKKG
                     ALKSADTLQKVTQELNKQITAKGGWKAISESTQAQLGNLKNSWNTTLDSIFRGSENGL
                     QDFTRSLTTLLNSLGGSGKSLGESLGSLMTSMSSGVDSLTDISYRVRAFFDEVTLAYR
                     GLTDEQKKVVDALGSGLINSLKVLAGVIAVNKVVGVTRSIYGLMTAITRLGQVADTTA
                     DNVNRKNGKPSRLNPMKMLLAGSAIYAGYERLASTPERMEAQAKLPELAKESGLKPAF
                     TMDDVKGWFNSAAMNLANFQHPELNNIKQDSALTSADIQSLRDEISALSKRIQEPVKV
                     SLGGEVAIKPDETSFMTFSSNIYDQYAEATLLSSSFPEDD"
BASE COUNT          487 a          256 c          345 g          424 t
ORIGIN      
        1 cgggtttaga tatcgctgaa ctgcaatcac agtttgctgg ttttggtgct tcagctaaag
       61 agtctatggg tattcaaggt tctgaagagc tttttagaaa tatgattggc tattctcgtt
      121 taatgggtcg aagtgaggaa gagattaagc gagcactgac agcattatca cagatggctg
      181 gtaagggtca gattatggct gaagagctta aggggcaact agctgaagct gttcctggta
      241 tggttcaggt attcgctaaa gcaaccggaa agagtgaaca ggaattattt gatgccatga
      301 agaagggggc tttaaaatct gccgatactc ttcagaaggt cactcaggaa ctgaataaac
      361 aaatcacagc taaaggtggc tggaaagcta tttcagaaag cacacaagca caactgggga
      421 acctgaaaaa ctcttggaat accacgcttg atagtatctt cagaggttct gaaaatggtc
      481 tgcaagattt tacacgaagc ctaacaacgc ttttaaacag tttaggtgga agtggtaaga
      541 gtcttggtga atctcttggt agcttgatga cttctatgtc aagtggtgtg gatagcctga
      601 ccgatatcag ttacagagtg agagcattct ttgatgaggt gacattagct tatcgtggtt
      661 taaccgatga acaaaagaaa gttgttgatg ctttaggttc tggcttgatt aatagtttaa
      721 aagtcttggc tggtgttatt gctgtgaata aagttgttgg tgtaaccaga agtatttacg
      781 ggcttatgac tgctatcaca agattaggtc aggtagctga tactacagcg gataacgtga
      841 atagaaagaa tgggaaacca tcaagactaa accctatgaa gatgctatta gctggtagtg
      901 ctatctatgc aggttatgaa agattagctt ctacacctga acgaatggaa gcacaagcta
      961 aattaccaga gctggctaaa gagtctggac ttaaacccgc tttcactatg gatgatgtta
     1021 aagggtggtt taatagtgct gctatgaatc ttgctaactt ccagcatcca gaattaaaca
     1081 acattaagca agattcagca ttaacgagtg cagatatcca aagcctgaga gatgaaatct
     1141 ccgctttgag caaacgtatt caggagcctg tgaaagtttc acttggtggt gaagttgcca
     1201 ttaaaccaga tgaaaccagc tttatgacct ttagcagtaa tatctacgac caatatgcag
     1261 aagctacgct attaagttca tcattcccag aagatgatta ataatagtcg aatgctacgt
     1321 gaataaagca aagttaatac ccacaccaaa aattattttt cacgtagctc acgcttaaca
     1381 gagaaaatac ttatatatca aaggtttggt taaaaatgat taaaatacac taagcctttg
     1441 aaatcaaagg agaatttaat gatttactct aatcaacttc gttacactgg caaagatggc
     1501 gtttacttcc ac
//
LOCUS       DAJTMC010000323         1496 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.333, whole genome shotgun sequence.
ACCESSION   DAJTMC010000323 DAJTMC010000000
VERSION     DAJTMC010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1496
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.333"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            <1..1494
                     /gene="pdeB"
                     /locus_tag="OO130_004247"
     CDS             <1..1494
                     /gene="pdeB"
                     /locus_tag="OO130_004247"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HCR5367395.1"
                     /translation="LPVGLSIWLAHQQVETSFIEELDTYSSRVAIRANKVATQGKDAL
                     QELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNVPQCSSLEHESPPDTFPEPG
                     KISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPASFIDVIPYSSWQIDAAIIGN
                     AHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILPFPEMNISIITWASTKMLQK
                     GWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQDAIENRDICVHYQPIVSLA
                     NGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPLTLLIIRSVFEDMGDWLRQH
                     PQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIALELTEREFADPKTSAPIISR
                     YREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVDALEYKNVTPHIIEMAKTLK
                     LKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDFLRWAEQHL"
BASE COUNT          397 a          351 c          366 g          382 t
ORIGIN      
        1 ctgcctgtcg gcttaagcat ctggctggcc catcagcagg tagaaacatc gtttattgaa
       61 gagctggata cctattcctc ccgcgtcgct attcgggcca ataaggtggc gacacaaggg
      121 aaagatgcgc tgcaagagct ggaaagatgg caaggcgctg cctgtagcga agcccatctc
      181 atggaaatgc gtcgggtatc ttacagttat cgctatattc aggaagtggt ttatatcgat
      241 aacaacgttc cccagtgttc gtctctggag catgaaagtc cgcccgatac cttccccgag
      301 ccaggtaaaa tttcgaaaga tggttatcgt gtctggttaa catcgcataa cgatttaggc
      361 attatccgtt acatggtcgc catgggaacg gcacattatg tcgtcatgat cgaccccgct
      421 tcctttattg atgtcattcc ctatagctca tggcaaattg atgccgccat tattggcaat
      481 gcccataacg ttgtcataac cagcagcgat gaaattgctc agggaattat taccaggcta
      541 caaaaaacac ccggtgagca tatcgaaaat aatggaatca tttacgatat cctgcccttc
      601 ccggagatga atatttcgat catcacctgg gcttcaacga aaatgttgca gaaaggctgg
      661 catcggcaag tctttatttg gttaccgctc gggttggtga ttggcctgct ggcagcgatg
      721 tttgtgctgc gtattttgcg ccgtatccag tcaccgcatc atcggctgca ggatgctatc
      781 gaaaatcgtg atatttgcgt gcactatcag ccgattgtct ccttagccaa tggcaaaatt
      841 gtcggtgctg aggcactggc gcgctggccg caaacagacg gtagttggtt gtcaccagat
      901 agttttattc cgctggcaca gcaaacaggc ctttctgagc cattgacgct actgattata
      961 agaagcgtct ttgaagatat gggcgactgg ctgcgtcagc atccacagca gcatatttcg
     1021 atcaatcttg aatccaccgt gctcacctcg gaaaaaatcc cgcaattgct gcgtgaaatg
     1081 atcaatcact atcaggttaa tcccagacag atcgcgcttg aactcactga acgcgagttt
     1141 gccgatccga aaaccagcgc cccgataatt tctcgctacc gggaggcggg ccatgaaatt
     1201 tatcttgatg attttggtac gggatattca agtttaagtt atttacagga tctggatgtc
     1261 gacattctga agatcgataa atctttcgtt gatgcgctgg aatataaaaa tgtcacgccg
     1321 catatcatcg aaatggcaaa aacactgaaa ctgaaaatgg tagcggaggg aatcgaaacc
     1381 agtaaacaag aagagtggct acgccagcat ggcgtgcact acggtcaggg ctggctctac
     1441 agcaaggcat taccgaaaga agatttctta cgctgggccg agcaacattt gtgaat
//
LOCUS       DAJTMC010000324         1485 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.334, whole genome shotgun sequence.
ACCESSION   DAJTMC010000324 DAJTMC010000000
VERSION     DAJTMC010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1485
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.334"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            1..845
                     /locus_tag="OO130_004248"
                     /pseudo
     CDS             1..845
                     /locus_tag="OO130_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            960..>1485
                     /locus_tag="OO130_004249"
     CDS             960..>1485
                     /locus_tag="OO130_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630-like element IS630 family transposase"
                     /protein_id="HCR5367396.1"
                     /translation="MPIIAPISRDERRLMQKAIHKTHDKNYARRLTAMLMLHRGDRVS
                     DVARTLCCARSSVGRWINWFTQSGVEGLKSLPAGRARRWPFEHICTLLRELVKHSPGD
                     FGYQRSRWSTELLAIKINEITGCQLNAGTVRRWLPSAGIVWRRAAPTLRIRDPHKDEK
                     MAAIHKALDECSAEH"
BASE COUNT          383 a          386 c          396 g          320 t
ORIGIN      
        1 atggctcgct acgaccttcc cgatgaggca tggaccatca tcaagcctct gttacctcct
       61 gaacccgcca caccacgggc cggacgccca tgggctgagc accgtaaaat catcaatggc
      121 atgttctggg tgttatgttc cggtgcacca tggcgcgatt tacccgaacg atatggggca
      181 tggaaaactg tttataaccg ctttaaccga tgatcaaagt caggcgtgat taatattatt
      241 ttcaacaggt tgctttcgct acttgatgca aacggcttta ttgactggtc tgccaccgcg
      301 ctggatggca gtaatatccg ggcgctgaaa tgtgccgccg gtgctcaaaa aaacatcccg
      361 atatcgacgg agataatggc ccgggtcgct ctcgcggcgg ttttggcacc aaaatccatc
      421 tggcaacaga cggaagtggc ctcccgttaa atatcgtgct aagccccgga caagctcatg
      481 aaagccagtt cgcgcaacga cttctggacg ggattggcgt tcagcgtcag aacggcagca
      541 tgaaacgccg tggacatgcg gtactggctg ataaagcgta ttcagggcgc gcattgcgca
      601 acgagctgaa aaataacggt ataaaggcag taatcccccg aaagtcaaat gagaaaatgg
      661 catcggatgg acgtgcacag cttgatcgtg atgcgtactg caatcgtaat gtcgttgaac
      721 ggtgctttgg gcggctgaaa gaatatcgcc gcatcgccac gcgttacgac aaaacggcga
      781 gaaattacct ggcgatggtg aaactgggct gcatccgact cttttatcaa cgcttacgta
      841 attaagggac acagcctaaa tagctgcgcg gaatagtaga tccctttgag ggaacttagc
      901 ccggattgtg cgatctgatc aatcgccaaa tcaaaacaaa tcaccaaccg gactgagcaa
      961 tgccgatcat agcaccaatt tcccgtgacg aacgacgcct gatgcagaaa gccatccata
     1021 aaacacacga taaaaattat gcccgcagac tgactgccat gctgatgctg caccggggcg
     1081 accgtgtcag cgacgttgcc agaacgctct gctgcgcccg ttcctctgtt ggacgctgga
     1141 ttaactggtt cacgcagtcg ggtgttgagg gactgaaatc attacctgcc gggcgtgccc
     1201 gtcgctggcc gtttgagcat atctgcacac tgttacgtga gctggtaaaa cattctcccg
     1261 gcgactttgg ctaccagcgt tcacgctgga gtacagaact gctggcaata aaaatcaatg
     1321 agataaccgg ttgccagtta aatgccggaa ccgttcgccg ctggttgccg tctgcgggga
     1381 ttgtgtggcg aagggctgcg ccaactctgc gtatccgtga cccgcataaa gatgaaaaga
     1441 tggcagcaat ccataaagca ctggacgaat gcagcgcaga gcatc
//
LOCUS       DAJTMC010000325         1409 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.336, whole genome shotgun sequence.
ACCESSION   DAJTMC010000325 DAJTMC010000000
VERSION     DAJTMC010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1409
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.336"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(<1..125)
                     /gene="ariR"
                     /locus_tag="OO130_004250"
     CDS             complement(<1..125)
                     /gene="ariR"
                     /locus_tag="OO130_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm/acid-resistance regulator AriR"
                     /protein_id="HCR5367397.1"
                     /translation="MLEDTTIHNAITDKALASYFRSSGNLLEEESAVLGQAVTNL"
     gene            complement(154..426)
                     /locus_tag="OO130_004251"
     CDS             complement(154..426)
                     /locus_tag="OO130_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YmgA"
                     /protein_id="HCR5367398.1"
                     /translation="MKTSDNERIKYEITGQVVLQILRMKINFSLQTLIKQLLVMKSAE
                     EDAFRRDLIDSIIRDFSNSDSGGPNRRTATADNKSMFNGKKINRIH"
     gene            complement(469..705)
                     /locus_tag="OO130_004252"
     CDS             complement(469..705)
                     /locus_tag="OO130_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YcgZ"
                     /protein_id="HCR5367399.1"
                     /translation="MHQNSVTLDSAGAITRYFAKANWPTQQETLGEIVTEILKDGRNL
                     SRKSLCAKLLCRLEHATGEEEQKHYNALIGLLFE"
     gene            1019..>1409
                     /gene="bluF"
                     /locus_tag="OO130_004253"
     CDS             1019..>1409
                     /gene="bluF"
                     /locus_tag="OO130_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
                     /protein_id="HCR5367400.1"
                     /translation="MLTTLIYRSHIRDDEPVKKIEEMVSIANRRNMQSDVTGILLFNG
                     SHFFQLLEGPEEQVKMIYRAICQDPRHYNIVELLCDYAPARRFGKAGMELFDLRLHER
                     DDVLQAVFDKGTSKFQLTYDDRALQFFR"
BASE COUNT          413 a          289 c          252 g          455 t
ORIGIN      
        1 attaaattgg tgacagcctg ccctaacact gctgactctt cttctaacaa attacccgaa
       61 ctgcgaaagt aacttgctaa cgctttatca gttattgcat tatgaattgt agtatcttca
      121 agcatggtaa cctctcatct tacttatgaa attttaatgt attctgttta ttttcttacc
      181 attgaacata cttttattgt cagctgtcgc tgttcttcgg tttggccctc ctgaatcact
      241 attgctaaaa tcacgaatta tgctgtcaat taaatcgcgt cggaaagcat cttcctcagc
      301 cgatttcatt acgagtaatt gcttaataag agtctgcaat gaaaaattta tcttcatgcg
      361 cagtatctgg agcaccacct ggccagtaat ttcatatttt atacgttcat tatcagatgt
      421 cttcataacg acttcatagc taacctgtta aataatcagg ctgttgcatt attcaaaaag
      481 caacccaatt agtgcattat aatgtttctg ttcctcttcc ccagtcgcgt gttccagtcg
      541 gcaaagaagt ttggcacaaa gcgactttcg actcagatta cgtccatctt tcaaaatctc
      601 agtcacaatt tccccgagag tttcctgctg agtaggccag ttagcttttg caaagtaacg
      661 agtgattgct cccgcagaat ctaaagtcac tgaattttga tgcatgctac gcctctgtta
      721 aaaatgttaa tcaatgttaa gtgaggtaac aatagcaaac ttgtacgaaa tatgtgtaca
      781 gaatgataaa ttaatttttt gcaattagtg ccaatacata taaaacaatg tgttatgata
      841 aaacatattg catcatttaa ctgacaatct atacgagatt tttcgccaac tagtgttcaa
      901 ctttttccat tttttatcaa tgtaaagaaa cggttatgtt ttggtattaa aagaaagaca
      961 caagaagata tgtctgttac cgtcttactc tcgcctcacc cattaccctg gattgttaat
     1021 gcttaccacc cttatttatc gtagccatat acgtgacgac gaacctgtca aaaaaatcga
     1081 agaaatggtt tcgatagcaa atcgcaggaa catgcagtct gacgtaacag ggatcttact
     1141 gtttaatggt tctcattttt tccagcttct ggaaggtccg gaagaacagg ttaaaatgat
     1201 atatcgggct atatgccagg atccacggca ctataatatt gttgagctgc tgtgcgatta
     1261 cgcgcctgct cgccgttttg gcaaagcggg aatggaatta tttgatttgc gcctgcacga
     1321 gcgagatgac gttttacagg ccgtattcga caaaggcaca tcaaaatttc agctaactta
     1381 tgatgacaga gcgctacaat tttttcgta
//
LOCUS       DAJTMC010000326         1396 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.337, whole genome shotgun sequence.
ACCESSION   DAJTMC010000326 DAJTMC010000000
VERSION     DAJTMC010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1396
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.337"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            6..689
                     /locus_tag="OO130_004254"
     CDS             6..689
                     /locus_tag="OO130_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HCR5367401.1"
                     /translation="MKTQHDHYDRLSVRLSVIISRLLAGESLELKALADEFGVSERTL
                     QRDFQQRLIHLDLEHSSGSYRLTCRTGREHSPNTFAFIRNSGISGIIPAQNRRLIRLI
                     TSNSGSSPCLIGHGGLPSPVTQTACFLELAEAIRDYRAVTLLVNGKSYEAVAPRRLIF
                     QSKNWFLVATRTRNLQVFRMEDVNAVTVLDTSFRRKPELDALTASEEFISALPHFRFI
                     SDVIQTFRE"
     gene            728..>1396
                     /locus_tag="OO130_004255"
     CDS             728..>1396
                     /locus_tag="OO130_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HCR5367402.1"
                     /translation="MNDPVKGEGRMITDASGVTRREGGILGMWSNIFPERNGSNEQKG
                     DSKPHIPDIRDILDIFPQPLREKLLNKISEAIDYEPVIGVMGKTGAGKSSVCNALFKG
                     EVCAVSDVEACTREVQELRIRFGKHSLKIIDIPSVGENARRDKEYEYLYRNLLPSLDL
                     ILWVIKGDDRAFSADEHFYNNVLLPAGGGERVLFVLNQVDKIEPFREWDTHLHQPSPA
                     QRINI"
BASE COUNT          364 a          331 c          346 g          355 t
ORIGIN      
        1 ccactatgaa aacacagcat gatcattatg accgcctgtc cgtcaggcta tcggtcatta
       61 tcagccgctt actggctggc gaatctctgg agcttaaagc gttagctgat gaatttggtg
      121 tctcagaacg caccctacag agggattttc agcaacgcct gatccatctg gatctcgagc
      181 attccagcgg aagctaccga cttacctgcc gaacgggccg ggagcactca cccaacacat
      241 ttgcgttcat ccgtaattcc gggatttcag ggattattcc ggctcagaat cgcaggttaa
      301 ttcgactgat taccagtaac tccggaagct caccgtgcct tatagggcat ggagggctgc
      361 catctccagt tacacaaacc gcctgttttc tggaactggc ggaagctatc cgtgactacc
      421 gcgccgttac attattagtt aacggtaaaa gctacgaagc agtagctccc cgccgtctga
      481 tatttcagtc caaaaactgg tttcttgttg ctacccgcac cagaaattta caggtgtttc
      541 ggatggaaga tgttaacgcc gtaaccgttc tggatacctc ttttcgcaga aagcctgaac
      601 ttgatgctct gactgccagt gaagagttta tttcagccct ccctcacttt cgtttcataa
      661 gtgacgtaat tcagaccttc agagagtaac agacaacgtt catttcaaat taaaaaggag
      721 acctcgtatg aatgacccgg taaagggcga aggccggatg attactgatg ccagtggcgt
      781 caccagaagg gaaggcggga ttttgggaat gtggagcaat atatttccag aacgtaatgg
      841 tagcaacgag caaaaaggtg atagcaagcc gcacataccc gacatcaggg atattcttga
      901 tattttccct cagccactgc gggaaaagct gttgaacaaa atcagtgaag ccatcgacta
      961 tgaacccgtg attggtgtca tgggaaaaac tggcgcgggg aaaagcagcg tatgtaatgc
     1021 gctgtttaaa ggggaggttt gtgcagttag cgacgttgag gcatgtactc gcgaagttca
     1081 ggaattgcgt atacgcttcg gcaaacattc gctaaagatc attgatatac ctagtgtcgg
     1141 tgagaatgcc cggcgggaca aggagtatga atatttatac cgtaatttgc ttccctccct
     1201 ggatcttatc ctgtgggtaa taaaaggaga cgatcgggcc ttttctgcgg atgagcattt
     1261 ctacaataat gttctgctcc ctgctggtgg gggtgagcgc gttttattcg tcctcaacca
     1321 ggttgataaa attgagccat tccgcgagtg ggatactcat ctgcatcagc catctcctgc
     1381 acagcgcatc aacatc
//
LOCUS       DAJTMC010000327         1333 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.339, whole genome shotgun sequence.
ACCESSION   DAJTMC010000327 DAJTMC010000000
VERSION     DAJTMC010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1333
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.339"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(<1..173)
                     /locus_tag="OO130_004256"
     CDS             complement(<1..173)
                     /locus_tag="OO130_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory CII family protein"
                     /protein_id="HCR5367403.1"
                     /translation="MFDYQVSKHPHFDEACRAFALRHNLVQLAERAGMNVQILRNKLN
                     PAQPHLLTAPEIWL"
     gene            complement(206..427)
                     /locus_tag="OO130_004257"
     CDS             complement(206..427)
                     /locus_tag="OO130_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HCR5367404.1"
                     /translation="MRPNISITLTTPHVTIERYSELTGLSIDTINDMLADGRLIRHRL
                     RKDKKREKVMINIAAMTVDALSECNLNLN"
     gene            553..1122
                     /locus_tag="OO130_004258"
     CDS             553..1122
                     /locus_tag="OO130_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001047328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein CI"
                     /protein_id="HCR5367405.1"
                     /translation="MNLEKGGRGAIERMVEAYGFKTRQALCDHLGISKSTLATRYMRD
                     SFPAEWVIQCALETGTSLNWLTTGHGSKQTSGNTNTMEVAKYVLSDGALREDGFYIFD
                     KGFLPSTFKKPFVITDNNSEFICDKEFDDIRDGKWVISIDGEVTIRDITRLPGGRIFV
                     EGGNRAFECKIEDIEIIGKIISLTVKYVR"
     gene            1138..1329
                     /locus_tag="OO130_004259"
     CDS             1138..1329
                     /locus_tag="OO130_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5367406.1"
                     /translation="MLGKVFFVVLSCSLLLSPLTTYAKNYPCSGKKGGVSHCTSDGKF
                     VCNDGTISKSKKICTKNSR"
BASE COUNT          397 a          241 c          299 g          396 t
ORIGIN      
        1 agccagattt ctggtgcggt taataaatga ggctgagctg ggttcagctt gttccgcaga
       61 atctgcacat tcatgcctgc acgttctgcc agttgcacca gattgtggcg cagtgcaaat
      121 gcacgacagg cttcatcaaa atgtggatgt ttggaaactt ggtaatcaaa catggtcaat
      181 gcctctgatg tatttcagaa tcgaactaat taaggtttag attgcattct gaaagcgcat
      241 caacggtcat tgctgctatg ttgatcatca ctttttcgcg ttttttatct ttgcgcagac
      301 ggtgacggat aaggcgtcca tcagccaaca tgtcattgat ggtatcgatg gatagccctg
      361 tcagctcgct atagcgttca atagtcacat gaggcgtggt aagagtgatt gaaatgttag
      421 gtctcatgat gcaacattcc tcgtttaatg atgattaatc aggacgaata cggatcgttt
      481 gtattttgtg aacaccataa acatacgatt gcacagtgaa atcgtcaaga taaaagttca
      541 cttggagtga ccatgaattt ggagaaaggc ggacgaggcg ccatagagcg catggtagaa
      601 gcttatggat tcaagactcg acaggcgttg tgcgatcatt taggaatctc taaaagtaca
      661 ctcgccacac gctacatgcg tgactcattc ccagcagaat gggtaatcca gtgcgccctt
      721 gaaacgggca cctcgcttaa ttggctcaca accggacatg gttcaaagca aacttcaggt
      781 aatacaaata ctatggaagt tgctaaatat gtattatctg atggtgcctt gcgtgaagac
      841 ggtttttata tttttgataa gggatttcta ccctctacgt ttaaaaaacc ttttgtcatc
      901 acagataaca attctgaatt tatttgtgat aaagaatttg atgacatacg tgatggtaaa
      961 tgggtaataa gtattgatgg cgaagtaacg atccgtgaca ttactcgttt acccggtgga
     1021 agaatcttcg tcgagggtgg aaacagagcc ttcgaatgta agatagaaga cattgaaata
     1081 attggtaaaa ttataagttt aacagttaaa tatgttagat aataccggga ggaaactatg
     1141 cttggtaaag tattttttgt ggttttatca tgctctttgt tattaagccc actaactacc
     1201 tatgctaaaa attatccttg ttctgggaaa aagggaggtg tctctcactg tacctccgat
     1261 ggaaaatttg tttgcaatga tggaactatt agtaaatcca aaaaaatctg tactaaaaac
     1321 tcacgataac ttt
//
LOCUS       DAJTMC010000328         1330 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.340, whole genome shotgun sequence.
ACCESSION   DAJTMC010000328 DAJTMC010000000
VERSION     DAJTMC010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1330
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.340"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(<1..>1330)
                     /locus_tag="OO130_004260"
     CDS             complement(<1..>1330)
                     /locus_tag="OO130_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HCR5367407.1"
                     /translation="GSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQMKFAGYDV
                     IIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTSATTEEICRLTSPETCVAAIGQAGE
                     NLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKRLNDYMMTE
                     LIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPPGNQNTVGF
                     RTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGNTCVANFVH
                     TTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYSKGVFKCVL
                     PAEEYAEIHWDQLEAGDVNFIKDFYYRLAHRVGELSHLADGSYAIAERWNLGEEYWGY
                     AKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGLPLK"
BASE COUNT          342 a          364 c          283 g          341 t
ORIGIN      
        1 tttcaatggc aagccggaac caataaagtt gatatgggta tgtgtcatgc agtcacggtt
       61 gaacatacag ttaacaatgg aaccgacctg cgctgacgct tcattggcat ggtgaaccgg
      121 atagccaaac ggcgaccaga gtttattttt cgcgtagccc cagtactctt cacccaaatt
      181 ccagcgttct gcgatggcat atgaaccatc agccaggtga ctcagctcac ccacacgatg
      241 cgccagacgg tagtaaaaat ctttaatgaa attaacgtca cccgcttcca gttgatccca
      301 gtgaatttcc gcatactctt cagcaggcag aacacacttg aacacccctt tgctgtaaca
      361 ataagtaaag tcgcgatgca actgcccgta gttacaccat aggccgtagt catcgaacaa
      421 attcagacca accaagttac caatcacacg gccatcgtct ttatcttcaa aatctttcgg
      481 accgttcggg aagatggtgg tatggacaaa gttggctaca caagtgttac cacctgtgct
      541 gggcacgcca aactctttca cccgaggaat attcatttgg gtcatacaac ggatcgggca
      601 agagtggcag ccactcattt ttactgtgta tttctctgcc gccggaccta agtcaaaaac
      661 ggatttatag gtacgaaagc cgaccgtgtt ctgattgcct ggcggaattt cccccgtttc
      721 aatcggacca ccttcagccg cgccccaaaa cagcccttta cgtgctgtcc aacgtgactt
      781 gggatctgaa tactctgccc acgattgcgg cgtacttggc acgacatggt tgttattcgc
      841 accaataagt tcagtcatca tgtaatcgtt gagacgcttc atctcctgac gatcggcaat
      901 gttgacccct ttcgtccctt caaccgcaat cgctttcagg tttttcgaac ccataattgc
      961 gccagttccc gcaccgccgc tgtggttacg gctattcaac atgccagaga gaggaacaag
     1021 gttttcccca gcctggccaa tagccgccac acaggtttcc ggactggtca atcgacaaat
     1081 ttcttccgtc gttgcgctcg tcccttttcc ccataagaaa tcggcttttt ccaggctaac
     1141 tttgtcatct ttaatcttca gccataccgg tgatttcgcc ttcccttcga taataatgac
     1201 gtcgtatcca gcgaatttca tttgcgctgc aaaaaagcca cccatatggg catcgacaac
     1261 taaatttcct ttggtaaaag tagaaagtga ggtgatattt acgcgagaac tacagggggc
     1321 accagatcca
//
LOCUS       DAJTMC010000329         1327 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.341, whole genome shotgun sequence.
ACCESSION   DAJTMC010000329 DAJTMC010000000
VERSION     DAJTMC010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1327
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.341"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(170..499)
                     /locus_tag="OO130_004261"
     CDS             complement(170..499)
                     /locus_tag="OO130_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HCR5367408.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(671..>1327)
                     /locus_tag="OO130_004262"
     CDS             complement(671..>1327)
                     /locus_tag="OO130_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCR5367409.1"
                     /translation="TGEIDTALWRSGDVLLGSLLAMLFTGIWPQRAFIHWRIQLAKSL
                     TEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIAPASKETRIPKSIYEGIQT
                     INRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQQILLSLVHALYEGNPQPV
                     FANTEKLNDAVEELRQLLNNHHDLKVVETPIYGYVWLNMETAHQLELLSNLICRALRK
                     "
BASE COUNT          326 a          310 c          311 g          380 t
ORIGIN      
        1 agtgcggtta ataacaatgg agttacagaa ctttttgcct tgctgcatac agaagatgtc
       61 tgtcgctaat ttcggtaagt tatcagatgg gaatgatgac ggtgatacac agaaaagaat
      121 caatgagcaa tgagggttgc caggcaaccc tcattgaata aaacaggaat tacttcgctt
      181 cgccgttttt catttcgccc atagctttca gctttttgga gatatcgcgg cgttctttgg
      241 agagctcggc atttttgatg atgtaatcgt caacgcgatc ttcatagtca cctttcatgc
      301 tggcgatgat gccctggatt gcttcaacgc tcatccccgg cttgatgtaa tcgctcaggt
      361 tgtccagcag caggacgcgc ttctggttgt cacggatctt tttctcaacg tcctgaattt
      421 cacgttgcag tttgttctta cgacggaaca gacgaacaaa ttccagtacg tcctggaatg
      481 aaggcttggt agtttccatt tttatacccc tgataatgtg agagtcggat tcgtttaagc
      541 aaccgctatc cgttagagcc aacattactc atagctgccg cagatgacaa tgcttttatc
      601 ccttttcact atcataccct ttatccttgc tgaatcgaag cagcagcaag atgattctgt
      661 agttcaggaa ttatttgcgc aaggcccggc aaatcagatt cgatagcaac tcaagctgat
      721 gtgccgtttc catgttcagc cacacataac catagattgg tgtttccaca accttcaggt
      781 catggtggtt attgagcaac tgacgcagct cttccactgc atcgttcaat ttttccgtat
      841 tggcaaaaac cggctgcgga ttaccttcgt acaacgcatg aacaaggctc agcagtattt
      901 gctgcatcat gtgctgggta tcacgaagtt tttgcgcgtt caataacacg aaatggctgg
      961 ggcgcgtggc ccagtacgca ttgatttgca actccagcat acaaaccaga ttgcggttaa
     1021 tggtctggat accttcatat atcgattttg gaatacgggt ttctttgctg gcgggcgcaa
     1081 tcagcccacg cattttcacg gcatcggtca ggagtttttg tagatggctt tccagacgtg
     1141 ggcgttcgag taagttcggt gagaatgcag attgatagac ccgattatac tcggtcagac
     1201 ttttcgccag ttgaatgcgc cagtggatga acgcccgttg tggccagata ccggtaaaca
     1261 acattgccag taaagagccg aggagcacat cgccgcttcg ccataacgcc gtatcaattt
     1321 cacctgt
//
LOCUS       DAJTMC010000330         1315 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.342, whole genome shotgun sequence.
ACCESSION   DAJTMC010000330 DAJTMC010000000
VERSION     DAJTMC010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1315
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.342"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            <1..744
                     /locus_tag="OO130_004263"
     CDS             <1..744
                     /locus_tag="OO130_004263"
                     /inference="COORDINATES: protein motif:HMM:NF026861.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS domain-containing protein"
                     /protein_id="HCR5367410.1"
                     /translation="YTPARKIHLYNCDHRGLPLALISTEGATEWCAEYDEWGNLLNEE
                     NPHQLQQLIRLPGQQYDEESGLYYNRHRYYDPLQGRYITQDPIGLKGGWNFYQYPLNP
                     ISEIDPQGLNPLILGVVALMSCGLFSSGGVIQSGQQRREQYGRKVNPEADKLTDEHKS
                     GVNPGGNCSPQNLSDLQNEKNRLCNQSRACAPQMLRKEILRRYEINLACASVRKQINN
                     QCFGGGDKAHMEEENKAYKTAADCSGLIK"
     gene            741..1244
                     /locus_tag="OO130_004264"
     CDS             741..1244
                     /locus_tag="OO130_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5367411.1"
                     /translation="MKKIIFSNESAVYDELMIHFPCQPLPHISNDIIGLDELDIVYNF
                     FQKKQWNEIANNLKIKDDSYALELGITFLPEKVFCYYIPLYIYVSLFNKNDFWVFESD
                     FIQQYLCPEYRDYDDFLNFVFNFSDIQLSIIAQFMSYESDAGFFYASKACMDFWEDYS
                     PLLHKKI"
BASE COUNT          416 a          228 c          292 g          379 t
ORIGIN      
        1 tacacgccgg cgcgaaaaat ccacctgtac aactgcgacc atcgcggcct gccgctggcg
       61 cttatcagca cggaaggggc aacagaatgg tgcgcagaat acgatgagtg gggcaacctg
      121 ctgaatgaag agaacccgca ccagctgcag cagcttatcc gtttgccggg gcagcagtat
      181 gatgaggagt ccggcctgta ttacaaccgc caccgctatt atgacccgct gcaggggcga
      241 tatatcactc aggacccaat tgggctgaag gggggatgga atttttatca gtatccgttg
      301 aatccgatct cagagataga cccacaaggg ttaaatccgt taattcttgg tgttgtagca
      361 ctgatgagtt gcggtttgtt ttcatcaggt ggtgttatcc aaagtgggca acaacgcaga
      421 gagcaatatg gacgtaaagt taatccagaa gccgacaaat taacagatga acataagtca
      481 ggtgtaaatc ctggaggtaa ctgtagtccc cagaacctga gtgacttaca aaatgaaaaa
      541 aacagattat gtaaccagtc tagagcttgc gctcctcaga tgctgagaaa agaaatattg
      601 agacggtacg agataaattt ggcgtgcgct tcggtcagaa agcagattaa taatcagtgc
      661 tttggtggtg gagataaggc ccatatggaa gaagagaata aagcatataa aacagcagct
      721 gattgcagtg gcttaataaa atgaaaaaaa taattttctc taacgagagt gcagtctatg
      781 atgaacttat gattcatttc ccatgccagc cattacctca tatttcaaat gatattatag
      841 ggctggatga actggatatt gtatataatt ttttccaaaa gaaacaatgg aatgaaatag
      901 ccaataattt aaaaataaag gatgactctt atgctttaga gttaggaatt acattcctcc
      961 ctgagaaagt attctgctat tacatccctc tgtatattta tgtctcgttg tttaataaga
     1021 atgatttttg ggtttttgaa tccgatttta ttcaacaata tttatgtcct gagtatcggg
     1081 attacgatga ttttttgaat tttgttttta atttttcaga tatacaacta tctattattg
     1141 cacagtttat gtcttatgaa tcagacgctg gattctttta tgcaagtaaa gcttgtatgg
     1201 atttttggga agattattca cctctattac ataagaaaat ttaatcgtaa aaggtataaa
     1261 tggtttaata aaaaacttgt ctactcgttg gagtagatga aaaataagat gacgt
//
LOCUS       DAJTMC010000331         1292 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.343, whole genome shotgun sequence.
ACCESSION   DAJTMC010000331 DAJTMC010000000
VERSION     DAJTMC010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1292
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.343"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            <1..909
                     /gene="pocR"
                     /locus_tag="OO130_004265"
     CDS             <1..909
                     /gene="pocR"
                     /locus_tag="OO130_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein PocR"
                     /protein_id="HCR5367412.1"
                     /translation="SAATLNSELINKIAQDFAQATSLAVVVVNIHGDEISELFNFTPF
                     CQMMRQHPQLSTRCRMSDRCGGLEASKSDQPCIYRCHAGLTDFSIPLVIAGHLVGFVL
                     CGQVRLRNDYVDLVDILNVDDCWQADPELLNEFRKVPEMDYSRVMASADLLKLIVENC
                     LKKQLNFVVIKENKSQSDSVRQTRAPSPHDNKMKKALRYIDAHLSDDLRLEDVASHVY
                     LSPYYFSKLFKKYHGIGFNAWVNQQRMASARELLCHSDWSIASIARNLGFSQTSYFCK
                     VFRQTYQVTPQVYRQQVSENAAMETV"
     regulatory      986..1161
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
BASE COUNT          348 a          278 c          321 g          345 t
ORIGIN      
        1 tctgcggcga cacttaattc agaactcatt aataaaatcg cgcaggattt tgcgcaggcg
       61 accagccttg cggttgtcgt ggtcaatatt cacggcgatg aaatttccga gctgtttaac
      121 ttcactccat tttgtcagat gatgcggcaa caccctcagc taagcactcg atgtcgcatg
      181 agcgatcgtt gtgggggcct ggaagcctct aaatccgatc agccttgtat ttatcgctgt
      241 catgccggat taaccgattt ctctattcca ctggtaatcg ccgggcattt agtcggtttt
      301 gtattgtgcg ggcaagttcg tctgcgtaat gattatgtcg atttggtcga tattctgaat
      361 gtcgatgatt gctggcaggc agatcccgaa ttacttaatg aatttcgcaa agttcctgag
      421 atggattact caagagttat ggcctctgct gatttgttga agttgattgt agaaaattgc
      481 ctcaaaaaac aactcaattt tgtggttatt aaagagaaca aatctcagtc agattcggtg
      541 cgccagactc gtgcgcctag tccccacgac aataaaatga aaaaagcttt acgctatatt
      601 gatgcgcatt tatcagacga tttgcgtctg gaagatgtcg cctcgcacgt ttatctaagc
      661 ccttattatt tcagtaagtt attcaagaaa taccatggaa tcgggtttaa tgcgtgggta
      721 aaccaacaac ggatggccag cgcgcgtgag ttactgtgcc atagtgactg gagcatcgcc
      781 agcattgcgc gtaatttagg tttttcgcaa accagttatt tttgcaaagt tttccgtcag
      841 acttatcagg ttacgccgca ggtgtatcga cagcaagtga gtgaaaatgc ggcgatggaa
      901 acggtataaa aaaagggaag gtttaaataa acgctggaat gaataattaa cgttatttac
      961 gtgttatggg aaaagtaaaa tcagctcata acatacgctg acaggtgtgg cacatttgtg
     1021 cctgggaaga gagtgaaaat ccctcgcagc ccccgctgct gtaatgctga caaacccgaa
     1081 aatgccactg atcgcgagat tgggaaggtc gggagaggat gacgctaagc cagaagacca
     1141 gccagtcagt caataccaac aacttcggtg ggaagcgggt tgcccggaag cgtccggtag
     1201 agtacaggcg cgtctttata cccctaccac cctgaaagga tcagggtatg gcggcatcat
     1261 ttagttcttt catcgcagtg ccgacaggca cc
//
LOCUS       DAJTMC010000332         1290 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.344, whole genome shotgun sequence.
ACCESSION   DAJTMC010000332 DAJTMC010000000
VERSION     DAJTMC010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1290
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.344"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(<1..152)
                     /locus_tag="OO130_004266"
     CDS             complement(<1..152)
                     /locus_tag="OO130_004266"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5367413.1"
                     /translation="MILRHRTGPAFQAIKDSLAVGLNALTLTDITKNATYGVEIESLV
                     LEINAPA"
     gene            complement(149..337)
                     /locus_tag="OO130_004267"
     CDS             complement(149..337)
                     /locus_tag="OO130_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HCR5367414.1"
                     /translation="MAFKHYDVVRAAPPSDLAEKLTHKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVVVSGATEPE"
     gene            598..933
                     /gene="iraM"
                     /locus_tag="OO130_004268"
     CDS             598..933
                     /gene="iraM"
                     /locus_tag="OO130_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HCR5367415.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKTFWNLIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQSP"
     gene            complement(1214..>1290)
                     /locus_tag="OO130_004269"
     CDS             complement(1214..>1290)
                     /locus_tag="OO130_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HCR5367416.1"
                     /translation="ADGVLVAGVVVVAFPLLKKKTPDC"
BASE COUNT          408 a          328 c          223 g          331 t
ORIGIN      
        1 gccggtgcat ttatctccag caccagactt tctatctcaa cgccatacgt tgcatttttg
       61 gtaatatccg tcagcgtcag tgcatttagt cccactgcca gactgtcttt tatggcctgg
      121 aatgccgggc cagtacgatg acgtagtatc actccggctc agttgcacca ctgaccacca
      181 catcaccttc tgctgcaatc gcctgcatca gggtataagg ggttatggcc accggactac
      241 caaacggctg ccagccctct ttcagtttat gtgtcagctt ttccgcaaga tctgacggcg
      301 gcgccgccct gacaacatca tagtgtttaa atgccatggt tctttccacc atctgaaaaa
      361 taattcttta aaatacctga catgtaatac agaaaaaaca caaaaccata ccttaaataa
      421 aaacctgatt atcaagcaga tatgcatgga taaactacaa gacgagatat aaaccaccct
      481 gcatttaaat aaacaataaa caacatcaga aaaataattc tgctctatgg tttacattca
      541 aaaatatcat ttatactttt cagaacatca ccagcaaggc ataaacaagg aaactaaatg
      601 aagtggattg tgattgatac agttatccag ccatcatgcg gaatatcttt ttcagtcata
      661 tggagtaaaa taaaattaat aatctggtat caatcggatg ctttcttacc tcctgaaagt
      721 atatttacac tgactcacac aggcatcatg ctcaataaca aagtgctacc tgtaaccatt
      781 tacaacgtag taccattcaa taaaacattc tggaatttaa tcaaaaacag ccaggaatgc
      841 cctacaaata cagataacgt attgaatgaa tgctttaata accgttgcac tctgcaaata
      901 tgtccttatg gactaaaaca acaaagtcca taaggagttt actcacatct gacaaaatca
      961 atataaacag cccctccgga gaggggctgg agagtggcgc tatgtgccat tgcatggtgc
     1021 cgggtgcctc ccggtgaatt cagtaccagc acctgaatcc gcgattatcc catataccta
     1081 ctcgctgatt gcccctccgc acagggggat tcaccatgcc agtttctttt aacaaactcc
     1141 ccgcaaacca gacaacagtc aaccgcctga attgtgaagt atttaaaaat ttctcccgct
     1201 aactgatacc cggctaacag tctggcgttt tctttttcag caacgggaaa gcaacaacta
     1261 ccacacccgc caccagcaca ccgtcagcca
//
LOCUS       DAJTMC010000333         1274 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.345, whole genome shotgun sequence.
ACCESSION   DAJTMC010000333 DAJTMC010000000
VERSION     DAJTMC010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1274
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.345"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(57..896)
                     /locus_tag="OO130_004270"
     CDS             complement(57..896)
                     /locus_tag="OO130_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCR5367417.1"
                     /translation="MLNVAIENQNGWNYSAPAPHKTGAGISTPKVTRAHNRAEAVFLC
                     VMHSHIQIMVRRAGQPQGWPGSRVTGSANPVRLTTHEISTSAGELINLSLEDVIMATT
                     LSHPDVTIENGRAVTTSISIAEFFGKQHHHVVQKIESFECSELFLTSNFSRVKFEHRG
                     NSYNAYQITKNGFVFLVMGFTGKKAATFKEAYIAEFDRMEAELRQNNTPPADKMIPGD
                     GRTLVVHFDKFGNVEFTETVPDGALVCTLETFRFYLEKQGWTLVNRGAIKNMTVEQLL
                     SLK"
BASE COUNT          330 a          313 c          296 g          335 t
ORIGIN      
        1 tgagggcagg tgtaaaacaa taatcaagca tggggttcat atccatagct ccatttttat
       61 tttaaggaaa gcaactgctc tacggtcatg tttttaattg cgccccggtt tacaagagtc
      121 cagccctgtt tttccagata aaaccggaaa gtctccaggg tacagaccag tgcgccatca
      181 ggaacggttt cggtgaattc gacattgccg aatttgtcga agtgaacaac cagagtgcga
      241 ccatcacccg gaatcatctt gtcagcaggt ggggtgttat tctggcgcag ttcggcctcc
      301 atgcggtcga actcagcaat gtaggcttcc ttgaatgtgg cggctttttt gcccgtgaag
      361 cccatcacca ggaaaacgaa gccgtttttg gtgatttggt aggcgttata agaattgccg
      421 cggtgttcga atttaacccg cgaaaagttg ctggttaaaa atagttcaga acattcgaag
      481 gattctattt tttgaacaac atggtgatgc tgcttgccaa aaaactcagc aatcgaaata
      541 gaagtagtga cggcgcgacc attttcgatg gttacgtcag ggtgagaaag ggtagtagcc
      601 ataatgacat cctcaagtga taagttaatt aactcaccag cagaggtgct aatctcatgg
      661 gtggtgagac gcacagggtt agcactaccg gtcactcgag aacccggcca gccttgcggc
      721 tgccccgcac gcctcaccat aatttgaatg tggctgtgca ttacgcataa aaaaaccgct
      781 tcagcgcggt tatgcgctcg agtaactttc ggggtgctaa tcccggcacc cgttttatga
      841 ggtgcaggtg cactataatt ccacccgttc tggttttcaa tagctacatt caacattttc
      901 tctttccttt catcaccgaa gtgaattttg tgatgcggtg cctagtgcct ccaggtgacg
      961 ttaaccagtt aacaattaca gtcggctttc ccacccaaac caataaggac taacatgact
     1021 tttaactgtg ccgcgtgcgc ttagccgcat tcaccgcatc acaaaattca ctttaaaaag
     1081 ggcggacatc agccagcaat taaaccgatg ccgccaactg gtacttcaca cagcaatgtc
     1141 gttatttaca accggaagcg cactcccacc atttaaattt cacagacaag accgactctt
     1201 tatgaatacc ggaaatgcgc cttcgtgttg tgcccggttt tatttcacca cctccgggct
     1261 ttggtggcct cggc
//
LOCUS       DAJTMC010000334         1253 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.346, whole genome shotgun sequence.
ACCESSION   DAJTMC010000334 DAJTMC010000000
VERSION     DAJTMC010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1253
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.346"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            13..435
                     /locus_tag="OO130_004271"
     CDS             13..435
                     /locus_tag="OO130_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HCR5367418.1"
                     /translation="MAKVFTQEEREKIKGQVLELVRQSGRETLRQLEAKTGATRYLMS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKLKKPSAVDPDLIWSLPDG
                     EIRRYDSRLNIICRECRKSEVMQRILSFYQGNFQEVMA"
     gene            432..737
                     /locus_tag="OO130_004272"
     CDS             432..737
                     /locus_tag="OO130_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4406 domain-containing protein"
                     /protein_id="HCR5367419.1"
                     /translation="MRVRVYIAGPMTGYKNFNREAFHNAEEELKREGHTVLNPAVLPD
                     GLTQPQYMDICMAMIRCVDAIYMLKGWQRSAGAKAELALAEKLGHAVIFQETASVQD"
     gene            724..1179
                     /locus_tag="OO130_004273"
     CDS             724..1179
                     /locus_tag="OO130_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HCR5367420.1"
                     /translation="MCKTNYQALRERYSPIQVPECPVCGDEMSIQRIFSRTHIVYACT
                     GEGDDGYFKTGRTFADEHYLKSRVTVVDVSAPDVLALLKELEVKDKRIAELTDALTQM
                     INAHKTTIRFGHERITECGGDCDSPEKMISENPDIRMAEAVLRAGIKTE"
BASE COUNT          361 a          250 c          350 g          292 t
ORIGIN      
        1 tcaggagggc ttgtggcaaa agtttttaca caagaagagc gggaaaaaat taaagggcag
       61 gttcttgaac tcgtacgcca gagtgggcgc gagacgttac gacaactgga agctaaaact
      121 ggggcaacaa gatatctgat gagcgttctg gccagagagc tggttgccag tggcgatgta
      181 tacaactctg gttacgggtt attcccgtct gaacaggcgc gtaaggactg gcaaaatgcc
      241 cgtaaaaagc tctcaagggc aaagctgaag aaaccatctg cggttgatcc ggaccttatc
      301 tggtcattac ctgacggaga aatacgtcgc tacgacagtc gcctaaacat aatctgtcgc
      361 gagtgccgga agagcgaagt tatgcagcgc atattgtcgt tttatcaggg taattttcag
      421 gaggtgatgg cgtgagggtg agagtctata tcgccggtcc aatgaccggg tataaaaatt
      481 tcaaccgtga ggcgtttcac aatgcggaag aggaactgaa acgggaaggg cataccgtct
      541 taaacccggc agtacttccg gacgggctga cacagccgca gtacatggat atctgcatgg
      601 cgatgattcg ttgtgtggat gcgatttaca tgctgaaagg ctggcagcgg tcagcaggcg
      661 ctaaggcaga actggcgctg gcggagaaac tggggcatgc ggtgattttt caggagacag
      721 ccagtgtgca agactaatta ccaggcatta cgtgaacgtt attcaccaat tcaggtgccg
      781 gaatgtcctg tttgtggcga tgaaatgtcg atacagcgca tattttccag aacgcatatt
      841 gtgtatgcct gcacaggtga gggggatgat ggatatttta aaactggtcg aacttttgcg
      901 gatgagcatt acctgaaatc gcgcgtaacc gtcgttgatg ttagcgctcc tgacgtactg
      961 gctttactga aagagctgga agttaaagac aaacgcattg cagaattgac agatgcgctt
     1021 acacaaatga ttaatgcgca caaaaccaca attcgttttg gtcatgaacg cataactgaa
     1081 tgtggtggtg attgcgactc gccggaaaag atgatttcag aaaatccgga tatcagaatg
     1141 gcagaggctg ttttgagagc aggaataaaa actgaataat taaatttagc acagcaaata
     1201 aaatttaatc cttaaccgga gggatttctg caccctcaga acatcaggag gcc
//
LOCUS       DAJTMC010000335         1234 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.347, whole genome shotgun sequence.
ACCESSION   DAJTMC010000335 DAJTMC010000000
VERSION     DAJTMC010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1234
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.347"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(340..606)
                     /locus_tag="OO130_004274"
     CDS             complement(340..606)
                     /locus_tag="OO130_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5367421.1"
                     /translation="MLTQTIFPCLPQKQENIILEVSNPVLLSSTVTTDGYTVFNKKAA
                     IYELQIPATNRTKTLKFTATEMQWLTKINEVGIVEKQSQRHSNI"
     gene            complement(788..1186)
                     /locus_tag="OO130_004275"
                     /pseudo
     CDS             complement(788..1186)
                     /locus_tag="OO130_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612066.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
BASE COUNT          322 a          248 c          254 g          410 t
ORIGIN      
        1 aaagccgtct atgggaagga gtctgctgtt attccagaga atggtaattc agtcatatat
       61 ttctccatct atgacttgtc aataccatcc tgatatttgt gggtcagcaa tatattctac
      121 taaaaggtat ttaaggtcat tgcgtccctg cactcgaata atggtgaaga aagcatgatg
      181 aatatatcac gataacgttc agtatgtatc gaggatagat ggagttactc ctctctgaaa
      241 tagaattact gctctacttt ttctgtttcc agaaattatt agtaacaaac cctgatatat
      301 tcttttgaaa atggagctta aacgagtctg aaattaactt cagatattag aatgccgttg
      361 gctttgtttt tcaacaattc ctacttcatt tatctttgtc aaccattgca tttctgtagc
      421 agtgaatttc aacgttttcg tccggttggt agcaggtatt tgtaattcgt atatagctgc
      481 ttttttattg aaaacagtat atccatctgt agtaacagta gaagataata atacaggatt
      541 tgacacttca aggatgatat tttcttgttt ttgtggaagg caagggaata tagtttgtgt
      601 aagcatattg cttccttatg ttatatttta gtaaagaatt tattttttac attatagtcg
      661 gctaaatgaa gtttactgta aaaaaacgat acttttttcg taagcgatga gatgtggagc
      721 cagaaaaatg gctgcgttac gtcattgaac tgtccttgac ccacgatatt cagacagtcg
      781 ttgtatctta agttgacatt acccgctgac gtcgatattc ccttggtgag agataaccca
      841 acgcactatg cggatggttt tcattgtaat gctcgatcgc cactgcaagg ttatgcaatg
      901 ccgttcttgg tttcggcatg aacgcgatgc agtcttcctt catcgttttc acgaaccgtt
      961 ctgctattcc attactctga gggctactga ctgccgtcgt gcagggctcc agattcaact
     1021 ctctggcgaa ctgccgtgtt tcatgagccg tataagccga accgttatcg gccactggat
     1081 tggccgttcc ggtcctttat cgccatatcg ctgacatgac atcctgcacc gttgctttgt
     1141 cataatctcc cgtgcctgct gcccagtcta tggcttcacg atcgcaatat ggaacgtaac
     1201 gctgtcatcg ggagaatcgt caatgcggca ttcg
//
LOCUS       DAJTMC010000336         1233 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.348, whole genome shotgun sequence.
ACCESSION   DAJTMC010000336 DAJTMC010000000
VERSION     DAJTMC010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1233
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.348"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(279..566)
                     /locus_tag="OO130_004276"
     CDS             complement(279..566)
                     /locus_tag="OO130_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HCR5367422.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVDDEDIIRLVAER"
     gene            complement(641..784)
                     /gene="mgrB"
                     /locus_tag="OO130_004277"
     CDS             complement(641..784)
                     /gene="mgrB"
                     /locus_tag="OO130_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HCR5367423.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            943..1182
                     /locus_tag="OO130_004278"
     CDS             943..1182
                     /locus_tag="OO130_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5367424.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
BASE COUNT          404 a          258 c          240 g          331 t
ORIGIN      
        1 taaaataaga aaaattgatg aatgagcaaa aaaatcaaga gagaaacgtt tcgctaataa
       61 ataataccac gcttaatcac aaatccgatg taaacatcct aataaattaa tgaggaaaat
      121 atctttttct gatatcccgt tacgcaaata aataatatga taattatttg aagtcagacc
      181 agagttttaa tttgaagcag ttgcaccatg acagtgcgta taatcaggat gaattgagta
      241 acccttcaac gacggggtta ctcaagattg aggaaggatt atcgttcggc gaccaggcga
      301 ataatatctt cgtcatcaac cgatttttga ctctcaatgg tctttttatc tgctttctct
      361 ggttcgtttg cttcgcacaa acgacgtaac agtgcatttt gacgtttttg ctgatccagc
      421 aacgcctcaa gcagttcaat ctgttcgtta gtccgtgaac tggcacgatt gataaaaaac
      481 cacaagatga gcccgataag aagaactacc accgatacaa ccaaagacgc aatgttcatc
      541 acgcctgaat ttacaacttc gttcatttca ccacctctga gtagaggcgc tattctacca
      601 ctgctggaga ggaagaaaat ctagtgctga aaaaatgata tcaccacggg ataaactggt
      661 taatggcaca aattccgctg aaaaattgta catcctgatc gcacatcatg ttgaatacct
      721 gcgcccaaag cagcaagcaa gccaacacca cgacaaccag aacgacccat cgaaactttt
      781 tcactccaat ctccgcttca cctaccttat gtcattaaac tagcatgcgt tagttaaaca
      841 gcgcctaact atgtcgatat tcgtgctttt tcggaatgag tagcttgctt cagcgcatat
      901 tgccgctacg attaagcgaa cataaaaaga gaagaggttg taatgcgatt catcattcgc
      961 acagttatgc tgatagcact ggtatggata ggattattac tcagcggata tggcgttttg
     1021 attggaagca aagagaatgc cgcaggatta ggtttgcaat gtacctatct gactgccaga
     1081 ggcaccagca cggtacaata cttgcacact aaaagtggtt tcttggggat aacagattgc
     1141 ccactgttac gtaaaagcaa tatcgtggtc gataatggtt aagggtaata aataaccaga
     1201 ttctcactca tgagtaaaat attcttatag caa
//
LOCUS       DAJTMC010000337         1225 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.349, whole genome shotgun sequence.
ACCESSION   DAJTMC010000337 DAJTMC010000000
VERSION     DAJTMC010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1225
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.349"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(1..483)
                     /locus_tag="OO130_004279"
     CDS             complement(1..483)
                     /locus_tag="OO130_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5367425.1"
                     /translation="MEINVTAPALLTDEHILQPFDCGNEVLSNWLRGRAMKNQMLNAS
                     RTFVICLEDTLRVVGYYSLATGSVTHAELGRSLRHNMPNPVPVVLLGRLAVDVCIQGH
                     GFGKWLLSDAIHRVVNLADQVGIKAVMVHAIDDDARAFYERFGFVQSVVAPNTLFYKV
                     "
     gene            complement(474..776)
                     /locus_tag="OO130_004280"
     CDS             complement(474..776)
                     /locus_tag="OO130_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCR5367426.1"
                     /translation="MEVFMSTAASVRKTPREHQINIRATDEERAVIDYAASLVNKNRT
                     DFIMELAYQEAKNIILDQRLFVLDNERYDSFITQLEAPVQNAEGRERLMAVKPEWK"
     gene            1048..>1225
                     /locus_tag="OO130_004281"
     CDS             1048..>1225
                     /locus_tag="OO130_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_145811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5367427.1"
                     /translation="MSHNLEHQKVHTRMVKEVLKAVARANNHPYKSVFADFITGHPSC
                     TVCFWETFHKMYPDS"
BASE COUNT          366 a          310 c          253 g          296 t
ORIGIN      
        1 tcagacctta taaaacaagg tattaggtgc cacaactgac tgaacaaaac caaaacgctc
       61 ataaaatgct ctggcgtcat catcaattgc atgtaccata acagccttaa tgccaacctg
      121 atcagccaga ttgacaaccc ggtgaatggc atcacttagt agccatttac cgaaaccatg
      181 cccctggata caaacatcaa cagccagacg ccctaacaga acaacaggga caggattggg
      241 catgttatgc cgcaaactgc gaccaagctc ggcatgagtg acggaaccag tagctaatga
      301 gtagtatccc acaacacgta aagtatcctc caagcaaata acgaacgtgc gagaagcatt
      361 gagcatctgg tttttcatag cccgaccacg taaccagtta cttagcacct catttccaca
      421 gtcaaatggc tgaagtatat gctcatccgt caacaatgct ggcgcggtga catttatttc
      481 cattcaggct ttaccgccat cagacgttca cgcccttctg cattctgcac aggagcttcc
      541 agttgcgtaa taaacgaatc ataacgttcg ttatccagca caaataaacg ttgatccagg
      601 ataatgtttt tagcttcctg gtacgccagt tccatgatga aatcggtacg gttcttattg
      661 accaagctcg cagcataatc aataacggct cgttcctcat cagttgcccg gatgttaatc
      721 tgatgttctc gcggtgtttt acggacgctt gcagctgtag acataaaaac ctccattggt
      781 aacaatttgc tatgcaatta atgttatcgc aaatggtaac agaacgctat acagaatcac
      841 ttacgggaaa agcgatcccc tgataaaaac aatggcagag acctgaacat tgtgacgatg
      901 atctgtgtcg gcgcacaaat catgtctgat tacggccgtt taatgtgatt ttgataatga
      961 aatgcctgaa ccacggcagc ctggcggctg caaatcgggg tgtggcagaa tatgagcttt
     1021 cagagtaacc atccagaatg agttcccatg tcacacaacc tggagcacca gaaagtccac
     1081 accagaatgg tgaaagaggt tctgaaagcc gtcgccagag ccaataatca cccttataag
     1141 tccgtatttg cagactttat caccggtcat ccctcctgca cggtctgttt ctgggaaacc
     1201 ttccacaaaa tgtacccgga ttcac
//
LOCUS       DAJTMC010000338         1199 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.350, whole genome shotgun sequence.
ACCESSION   DAJTMC010000338 DAJTMC010000000
VERSION     DAJTMC010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1199
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.350"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(<1..755)
                     /gene="ipaH4.5"
                     /locus_tag="OO130_004282"
     CDS             complement(<1..755)
                     /gene="ipaH4.5"
                     /locus_tag="OO130_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH4.5"
                     /protein_id="HCR5367428.1"
                     /translation="MKPINNHSFFRSLCGLSCISRLSVEEQCTRDYHRIWDDWAREGT
                     TTENRIQAVRLLKICLDTREPVLNLSLLKLRSLPPLPLHIRELNISNNELISLPENSP
                     LLTELHVNGNNLNILPTLPSQLIKLNISFNRNLSCLPSLPPYLQSLSARFNSLETLPE
                     LPSTLTILRIEGNRLTVLPELPHRLQELFVSGNRLQELPEFPQRLKYLKVGENQLRRL
                     SRLPQELLTLDVSNNLLTSLPENIITLPICTNV"
BASE COUNT          341 a          215 c          259 g          384 t
ORIGIN      
        1 ttaacattcg tacaaatggg caatgtgatt atattttcgg gtaatgaagt tagtaggtta
       61 ttggaaacat ccagtgtcaa tagttcttgc ggtaatctgg ataatctgcg tagttgattt
      121 tcacctacct tcaaatattt taagcgctga ggaaattctg gtagttcctg tagcctgttg
      181 ccggaaacaa agagttcttg tagtctatga ggcaattcag gcaagacagt aaggcgatta
      241 ccttcaatac gtaatattgt tagcgttgat ggaagctctg gtaacgtctc cagactatta
      301 aaacgtgccg agagtgattg taaatatggt ggtaatgatg gcagacatga caaatttcga
      361 ttgaatgaaa tattaagctt aatcagttga gatggaagtg tcgggagtat attcaagttg
      421 ttaccattta catgaagttc tgtcaaaagc ggagaatttt caggtaggga gattaactca
      481 ttgttggaaa tattaagttc acgtatatgc aaagggagtg gtggtaaaga acgtagtttc
      541 agtaagctta aattgagaac aggctcccgg gtatccagac atattttcaa taatcgaacc
      601 gcctggatgc gattttctgt tgttgttcct tccctagccc agtcatccca gatgcggtgg
      661 taatctctgg tacactgttc ttctaccgat aaacgagata tacatgataa gccacaaagg
      721 gaacgaaaaa aagaatgatt gttgatcggt ttcatttatt aacccctcac tattcatatc
      781 ttggtctatt aatgcaaata aaacgagaaa gtctttaaaa aaacaatcct agagctttat
      841 cggcgttttc attccagctg gaggaatatg ttcctgtctt tccagtaacc gtcgattgtt
      901 atatcagtct acccatgtta atgtgaccag ttctactttc atccagtttg gattgatctt
      961 tatcccctat attttcaaac aatttttatc atttaactca ttactgtctc gatattccca
     1021 tagcaaacga tctggttggt tagcccctca aaaaagtcag ttgcacgccg taaatccctg
     1081 ccggtattga gcgaggattt acgggttgtt gttaaggtcc gtaatcgggt cacagttttt
     1141 ccggagtcaa tccggtctgc ggtttatgct tatgcgacgt gattatgaat ggtgcagtc
//
LOCUS       DAJTMC010000339         1193 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.351, whole genome shotgun sequence.
ACCESSION   DAJTMC010000339 DAJTMC010000000
VERSION     DAJTMC010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.351"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(<1..77)
                     /locus_tag="OO130_004283"
     CDS             complement(<1..77)
                     /locus_tag="OO130_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HCR5367429.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKS"
     gene            266..796
                     /gene="cybB"
                     /locus_tag="OO130_004284"
     CDS             266..796
                     /gene="cybB"
                     /locus_tag="OO130_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HCR5367430.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            1041..>1193
                     /locus_tag="OO130_004285"
     CDS             1041..>1193
                     /locus_tag="OO130_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5367431.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISAS"
BASE COUNT          319 a          258 c          270 g          346 t
ORIGIN      
        1 ttgcttttga cttcaagtaa tcgacgcaac accagtcgac cgatacgacc aaaaccgtta
       61 ataccaacct tactcatggt tttctcctgt caggaacgtt cggatgaaaa ttgatccttt
      121 ccaggcttag cccaggattg tgggatgggc aatctccatt cttacagtga aacgtaacgt
      181 actgaaaacg ggtgaacaat atttaatgaa attttgagaa aagcccgtta tgttaacgga
      241 aaattatgtt aaagcaggaa atgttatgga aaataaatac tcaaggttac aaatcagcat
      301 tcactggctg gtctttttgc tggttatcgc agcgtattgc gcaatggagt ttcggggttt
      361 cttcccacgt agcgatcggc cactcatcaa catgattcat gtttcctgcg gcatctcaat
      421 tctcgtgctg atggtcgttc gtcttttgtt aaggctgaaa tacccaaccc cgccgattat
      481 acctaagcca aaaccgatga tgacgggact ggcgcatttg ggacatttgg tgatttatct
      541 cctgtttatt gcgctgccag tgattggttt ggtgatgatg tataaccggg gcaacccgtg
      601 gtttgcgttt ggtttgacga tgccttacgc ttcagaggcc aatttcgaac gggttgatag
      661 cttaaagtcg tggcatgaaa cgctggcgaa tctgggatat tttgtcatcg ggttgcacgc
      721 tgcggctgca ctggcacacc actatttctg gaaggacaac acacttctac gcatgatgcc
      781 gcgtaaacgt tcctgaagga tatttaaaga aaacgcctgt actaaaaccg acccgtggta
      841 caggcgaaga atacgggtct acatcggaag cgcctattat atttatttgt atgacaaata
      901 aaaccataat ccttgcccat acgtccatct ggcttatttt taatcaattc acccgatctt
      961 tgatctcatc aacggtatca aaataaacca gcgtataacg ttcaaatcga acataagatt
     1021 cgataaacca tggaggttat atgaaaaaac tggcacttat tttgtttatg ggaacgcttg
     1081 tttcctttta tgccgatgcc gggcgcaaac cctgttctgg ttcgaaaggg gggatctcac
     1141 actgtacggc aggcggcaaa tttgtctgta atgatggttc tattagtgcc tcg
//
LOCUS       DAJTMC010000340         1166 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.353, whole genome shotgun sequence.
ACCESSION   DAJTMC010000340 DAJTMC010000000
VERSION     DAJTMC010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1166
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.353"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(<1..313)
                     /locus_tag="OO130_004286"
     CDS             complement(<1..313)
                     /locus_tag="OO130_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5367432.1"
                     /translation="MADKAILWALISASTKEERKACSLSYFACKAAEAELGLAYMAAN
                     DNKEFLTSLSNIMRYKIDAGLSESYTCYLLSKGKIIRPYLKNLNPLQLAADCIETVNK
                     IK"
     gene            complement(1021..>1166)
                     /locus_tag="OO130_004287"
     CDS             complement(1021..>1166)
                     /locus_tag="OO130_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCR5367433.1"
                     /translation="LSEYYNLNRAIYWMEFAVNNGNIDAKSKLQELKKLKRMDRRKNK
                     ENP"
BASE COUNT          338 a          240 c          197 g          391 t
ORIGIN      
        1 cttttatttt atttactgtt tcaatgcaat cagcggcaag ttggagtgga tttagatttt
       61 tcagatatgg tcttatgatc tttcccttac ttaataaata gcatgtatag gattcggaaa
      121 gtccagcatc tattttgtac ctcattatat tagataagga tgtgagaaat tccttgttat
      181 cattcgcagc catatatgcc agtccgagtt cagcctctgc ggccttacag gcaaaatagc
      241 ttagtgaaca cgcttttcgt tcctctttag tactggctga tattagtgcc cataatattg
      301 ctttgtctgc caaatctaac tctctctgaa attgtcttgc agtaactgca ctcgcagaaa
      361 tagagaaaaa aatacaattg ttaataacct tgccatattt ttttagtccg taactgttaa
      421 tgtgctatct ggctctgaaa atattaactc atttaatttt tgcatgtcat tagataaacc
      481 agttatacaa ccctcggaac agaatcgagg tcctaaagac ggaccaaaag cgtttaaaca
      541 actatgtatt gaaaatccat tgcgattata agttcctgta ttaactgacg gtactaatcg
      601 tatgccgtca ggttttctat tgcttgctcc cgggtcatta acattccaac tccataggcc
      661 ttgaggtatt ggccatctat ttataaatgt gtacaacggg gattattttt acattgcata
      721 ttattaccag aagcgactgg aatactaagc acatcataaa aattatcatc acatggtata
      781 tctgaaacac acgaaagcat acctaaagtt atgctgtata aacaggtggc taatcccaag
      841 gggatcgatt ccagaaatcg ggttcaacgg atactgataa aaattccacc taccttccag
      901 cccaatcgga gcctgagtgt tataccgccc ctgctgcggt taatgttacc gataatagtt
      961 atagtacagc cctgattcat catcatacta ctgctgcggc agtctgatga actattgctt
     1021 ttacggattc tccttattct tccttctatc cattcgcttt agctttttga gctcttgaag
     1081 tttgctcttt gcatcaatat taccgttatt tacagcgaat tccatccaat aaatagctct
     1141 attcaaatta taatattctg ataatg
//
LOCUS       DAJTMC010000341         1160 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.354, whole genome shotgun sequence.
ACCESSION   DAJTMC010000341 DAJTMC010000000
VERSION     DAJTMC010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1160
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.354"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            1..567
                     /locus_tag="OO130_004288"
                     /pseudo
     CDS             1..567
                     /locus_tag="OO130_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
     gene            complement(585..1086)
                     /gene="tnpA"
                     /locus_tag="OO130_004289"
                     /pseudo
     CDS             complement(585..1086)
                     /gene="tnpA"
                     /locus_tag="OO130_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            1094..>1160
                     /locus_tag="OO130_004290"
     CDS             1094..>1160
                     /locus_tag="OO130_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence protein"
                     /protein_id="HCR5367434.1"
                     /translation="MKRLQAFKFQLRPGGQQECEMR"
BASE COUNT          335 a          258 c          270 g          297 t
ORIGIN      
        1 ttaaacaatt actctgattt aaaagaaaca acctcagcaa caggtaaacc tgttttacgt
       61 tgggtagacc cgagttttga tcaaagcaaa tatgacagca tcgtctggaa cccaatcact
      121 tattatccgg tgccaaaacc ttctacccag gtagggcaga aagttctgga taaaattttg
      181 aactatacca acaccgaaat gaaagaagcg atagcgcagc gtaaaccact ggttaccacc
      241 gctgggccgc gtagtctgat tttccgtggg gccattaccg gtgtagatac cagcaaagaa
      301 gggctgcaat tctatgaagt ggttcctgtt gcattagtgg ttgcggggac gcaaatggct
      361 acaggtcacc gtaccatgga tactcgcctc tattttgaag gtgagctgat tgatgcagcg
      421 actaataaac cggttatcaa agtcgttcgt cagggctaag gtaaagacct gaataacgaa
      481 agtacgccaa tggctttcga aaatattaaa caagttattg atgacatggc gaccgatgcc
      541 accatgtttg acgttaacaa aaagtagttc cagacgcgcc atcgttagat ggcgcttttt
      601 atccggtgcg ccgtaaaacc ccatccttca gggcggggat ataaggcgcg gttttccacc
      661 tgaccaggtg tttgctgttg ttcaatgtat tggcggatga cggatattgg cgcacctccg
      721 cagctactgg caaaatagcc aggactccac aaaacgcctt tgtagtaata cctgactgca
      781 atatctggtc gatctcgtcg cagtaaccta cccgatacgc ctttgaggct gtttaccaga
      841 ctggatatcg ccagtttggg aggttagtta attaacaaat ggacgtgatc tggttcgcca
      901 tccatttcaa ccagttcagc ttcaaaataa gcacatacat ttgaaaagta agtgcgtagt
      961 ttttctgtcg cgtcaaaaaa tctggcgtcg gtatctggtg acaaagacca ggtgaacatg
     1021 catcaggaaa acacaatgcc ttccacgccg gatatcggtt tcttttttca caaaccaaag
     1081 tatcatttga cctgtgaaac gattacaggc atttaaattc cagttaagac caggtggtca
     1141 acaggagtgt gaaatgaggc
//
LOCUS       DAJTMC010000342         1154 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.355, whole genome shotgun sequence.
ACCESSION   DAJTMC010000342 DAJTMC010000000
VERSION     DAJTMC010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1154
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.355"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(1..263)
                     /locus_tag="OO130_004291"
                     /pseudo
     CDS             complement(1..263)
                     /locus_tag="OO130_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(378..1016)
                     /gene="ospI"
                     /locus_tag="OO130_004292"
     CDS             complement(378..1016)
                     /gene="ospI"
                     /locus_tag="OO130_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspI"
                     /protein_id="HCR5367435.1"
                     /translation="MINGVSLQGTAGYEAHTEEGNVNVKKLLESLNSKSLGDMDKDSE
                     LAATLQKMINPSGGDGNCSGCALHACMAMLGYGVREAPVPNEISEYMTGFFHRHLEQI
                     DSEGIVSHPNETYSKFRERIAENILQNTSKGSVVMISIEQATHWIAGFNDGEKIMFLD
                     VQTGKGFNLYDPVEKSPDAFVDENSSVQVIHVSDQEFDHYANSSSWKSKRLC"
BASE COUNT          338 a          283 c          176 g          357 t
ORIGIN      
        1 agcaagcgct gtaactcttt tatttgtttt atagcggcag ccagttctga ggcaggaacg
       61 acatcctctc cggcagcaac agcagtgaga cttccttcca gatattattt tttccactta
      121 agcaacaggc tgggctgaat accatgcaaa cgcgccacat gggacaccgt catgccaggc
      181 tccattgtct gctgaataat ggcaattttt tcctgaggag ttttacgttt acgaagctcc
      241 agccccagca aagttccagt catctcaaaa ttagaactag tgttagacat atatccaagc
      301 ctatctctta ttttaagata catccactgt ctggcgtttc aaggggccgg ctcaaatcta
      361 tgtacaggct ctcagcatca gcaaagcctc ttactcttcc aactactact gttagcatag
      421 tgatcaaatt cctgatcaga tacatgaatt acctgaaccg aactattttc atctacaaat
      481 gcatctggcg atttttctac aggatcataa agattaaatc cctttcctgt ttgaacatcc
      541 aaaaacataa ttttttcacc atcgttaaat ccagctatcc aatgagtggc ctgttcaata
      601 gaaatcatta ctacacttcc ttttgaagtg ttttgtaaaa tattttctgc aatacgctca
      661 cgaaatttac tataagtttc attaggatgt gacactatgc cttcactatc aatttgctct
      721 aaatgcctat gaaaaaatcc ggtcatatac tctgatattt cattaggtac gggagcctct
      781 cttactccat accctaacat agccatacaa gcatgaagcg cacaccctga acagttacca
      841 tccccaccag acggatttat cattttctgt aaagtcgctg ccaattcaga atctttatcc
      901 atatcaccaa gacttttaga gtttaaagat tccagcaatt ttttaacatt tacatttccc
      961 tcctctgtat gtgcctcata tccagcagtt ccctgtaacg acaccccatt aatcatattg
     1021 tatcctgaca gtatatatac tcactatatt ttcttcataa caaatatccc tctgatatga
     1081 caacaaaaaa cggagccgga ttcctgtttt tgtgaactgc agctattcga gtctaaaaag
     1141 tgatccactt acca
//
LOCUS       DAJTMC010000343         1151 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.356, whole genome shotgun sequence.
ACCESSION   DAJTMC010000343 DAJTMC010000000
VERSION     DAJTMC010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1151
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.356"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(<1..318)
                     /locus_tag="OO130_004293"
     CDS             complement(<1..318)
                     /locus_tag="OO130_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCR5367436.1"
                     /translation="MKACLLLFFYFSLIDQLHGADVKIKENESVMGSTAMTYDLSEEK
                     LMKLKYKSQHGDSEASFRLYQYYCFTKNNIDKQLRFLERSASQGNVTAQFNYGVFLSD
                     TNPT"
     gene            complement(330..>1151)
                     /locus_tag="OO130_004294"
     CDS             complement(330..>1151)
                     /locus_tag="OO130_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCR5367437.1"
                     /translation="YTPARKIHLYHCDHRGLPLALISTEGATAWCAEYDEWSNLLSDE
                     NPHHLQQLIRLPGQQYDEESGLYYNRHRYYDPLQGRYITQDPIGLKGGWNFYQYPLNP
                     VINVDPQGLVDINLYPESDLIHSVADEINIPGVFTIGGHGTPTSIESATRSIMTAKDL
                     AYLIKFDGNYKDGMTVWLFSCNTSKGQNSFASQLAKELHTNVIGPDTLWTWWGRGTNG
                     KLKMDTVLTAPTNLNSNKDLMAITTKDLGNWITYGPSGHPISNMQGTPEKPSDIR"
BASE COUNT          305 a          262 c          220 g          364 t
ORIGIN      
        1 tgttggattt gtatctgata aaaagacccc ataattaaac tgcgctgtaa cattcccctg
       61 agatgctgat ctttccaaga atcgcaattg tttatcaata ttatttttag taaagcagta
      121 atattgatat agtcgaaatg atgcctctga atcaccatgt tgtgatttat acttcagttt
      181 catcagcttt tcttcactta aatcataggt cattgctgta gatcccatca cactttcgtt
      241 ttcttttatt ttcacatcag caccatgcaa ttgatcaata agagaaaaat aaaaaaatag
      301 taacaagcat gctttcatac atctacaacc tatcttatat cactgggttt ttctggcgta
      361 ccttgcatat tagaaatggg gtgcccagat ggcccatatg ttatccaatt accaaggtct
      421 tttgttgtta tagccattag atccttattt gaattaaggt tcgttggtgc tgttagcact
      481 gtatccattt ttaacttacc attagttcct cgcccccacc acgtccatag cgtgtcaggt
      541 cctattacat ttgtatgtaa ctctttagct aattggctag caaatgaatt ttgtccttta
      601 cttgtattac aagaaaataa ccaaactgtc atcccatctt tataattccc atcaaattta
      661 attagatatg ctagatcttt agctgtcatg atactgcgcg ttgcggattc aatagatgtg
      721 ggggtaccat gccccccgat tgtgaaaacg cctgggatat taatctcatc agctacagaa
      781 tggataagat cactttcggg gtataaattt atatcaacca aaccttgcgg atctacattt
      841 atgaccggat tcaacggata ctgataaaaa ttccatcccc ccttcagccc aatcggatcc
      901 tgagtgatat accgcccctg cagcgggtca taatagcggt ggcggttgta atacaggccg
      961 gactcctcat catactgctg acccggcagg cgaatgagct gctgcagatg atgcgggttc
     1021 tcatcactca gcaggttgct ccattcatca tattctgcgc accacgctgt tgccccttcc
     1081 gtgctgatga gcgccagcgg caggccgcga tggtcgcagt ggtacaggtg gatttttcgc
     1141 gccggcgtgt a
//
LOCUS       DAJTMC010000344         1147 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.357, whole genome shotgun sequence.
ACCESSION   DAJTMC010000344 DAJTMC010000000
VERSION     DAJTMC010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1147
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.357"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            <1..238
                     /locus_tag="OO130_004295"
     CDS             <1..238
                     /locus_tag="OO130_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HCR5367438.1"
                     /translation="PEKFFKMVRYFGFLANRVCGEKLPQVYRALGMDKPEPVAKVCYA
                     QMVKQFLSRDPFECVLCGGRMVYLRAIAGLNVEG"
     gene            complement(458..553)
                     /locus_tag="OO130_004296"
                     /pseudo
     CDS             complement(458..553)
                     /locus_tag="OO130_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858351.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(553..636)
                     /locus_tag="OO130_004297"
                     /pseudo
     CDS             complement(553..636)
                     /locus_tag="OO130_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
     gene            complement(666..995)
                     /locus_tag="OO130_004298"
                     /pseudo
     CDS             complement(666..995)
                     /locus_tag="OO130_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146522.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
BASE COUNT          296 a          263 c          301 g          287 t
ORIGIN      
        1 cccggagaag ttttttaaga tggtgaggta cttcgggttc cttgccaacc gggtgtgtgg
       61 agagaagctg ccgcaggtgt accgtgctct ggggatggat aaaccggaac cagtggcgaa
      121 agtgtgctat gcacaaatgg tgaaacagtt cctgagtcgt gacccgttcg aatgcgtgct
      181 gtgtggcggc cggatggtat acctccgggc catcgcggga ctgaatgtgg agggctgaag
      241 aaaaacgcgc gggatatcag tctgctgagg tatatgccgg cctgacacag gctaagtgtg
      301 cccgggaatc ggtgcaagta taaaaaacag cctgttgcgg tataagaatc gaccatgcag
      361 agagagaaaa caccggagtg gagggaaaag cagaaaagct cacgtggcat aaggcgtgga
      421 cagcggtatc gtctggtttt tcaatttcct atacgtgtac cattctggcg gcagcaggtt
      481 tactggccag caccagaaaa tcatataacc gcgccaaatc agaaagcgcc ttactggtcc
      541 acttctgttc catcagcgtg gcaccggtaa cggattgtca gtactgaggc tgtccgccca
      601 ggcctgtaca gcctgatggt cgataatctg tccggatgga ccagcgggag catttgaaca
      661 gaaacttacg gttttgctct ttcggtaggc tcagtaccgt aaggttctcc atcaacaaaa
      721 taagtaccat taggtaatac gtgtttatag ggcagtaatg atatatgggc taatacgtca
      781 tcatcaataa cctcaccatt ttcacgtaac ccttcaacaa tattgcttat tcttgatgta
      841 ttccagtaca ggattgcatt cgataccaga ctcagggcgc tggccttatt catgatttct
      901 tcgtaatcac cggtcgtgaa ttcaccctga tttgcaaaaa agatccatcg gggtaattta
      961 tgtcgatatt cgcctttatt gagctgaagc tcgagtaggc agcctgacgg ctgcggcttg
     1021 tcatggtctg caattaccgt tataaaaaca ggcaatgtca ttgtctttca ggtggttata
     1081 tggcccgttc agctaaaccc cgtaaacgta aacccgcctc tcaaagaagc aaacttcccc
     1141 gctatgt
//
LOCUS       DAJTMC010000345         1136 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.358, whole genome shotgun sequence.
ACCESSION   DAJTMC010000345 DAJTMC010000000
VERSION     DAJTMC010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1136
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.358"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(<1..823)
                     /locus_tag="OO130_004299"
     CDS             complement(<1..823)
                     /locus_tag="OO130_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR5367439.1"
                     /translation="MLPTNNNHRLISNSFSTYSIDTSRAYENYLTHWTEWKNNRIQEE
                     QRDIAFQRLVSCLQNQETNLDLSELGLTTLPEIPPGIKSINISKNNLSLISPLPASLT
                     QLNVSYNRLIELPALPQGLKLLNASHNQLITLPTLPISLKELHVSNNQLCSLPVLPEL
                     LETLDVSCNGLAVLPPLPFSLQEISAIGNLLSELPPLPHNIHSIWAIDNMLTDIPYLP
                     ENLRNGYFDINQISHIPESILNLRNECSIDISDNPLSSHALQSLQRLTSSPDYHGP"
BASE COUNT          323 a          163 c          274 g          376 t
ORIGIN      
        1 gcgggccgtg gtagtccggc gaagaggtta atctttgcag ggattgcaga gcatgggatg
       61 acaatgggtt atcactaata tctattgaac attcattcct cagattaaga atgctttccg
      121 ggatatgact tatctgattt atgtcaaaat aaccgttcct taaattttcc ggcaggtatg
      181 gaatatcggt taacatattg tcgattgccc atatggagtg aatgttgtga ggtagagggg
      241 ggagttcact aagaagattc cctattgcgc taatctcttg taaagaaaat ggtaaaggtg
      301 gtaaaactgc cagcccatta catgatacat ctaatgtttc cagtagttct ggtaaaacag
      361 gaagagaaca taattgatta tttgagacat gaagctcctt caaagatatg gggagtgtgg
      421 gtagtgtgat tagttgattg tgggacgcat tcaataattt aagtccttga ggcaaagcag
      481 gcagttcaat aagtctgtta tagctgacat taagctgtgt aagggacgca ggcaatgggg
      541 agattaagct taaattattt ttacttatat taattgattt aattcccggg gggatttcag
      601 gtaatgttgt caggcctaat tcagacaagt ctaggttcgt ctcttggttt tgtagacatg
      661 atactagtcg ctgaaaagcg atgtctcgtt gttcttcttg tatgcggtta tttttccatt
      721 cagtccaatg ggttagataa ttttcatatg cgcggctagt gtcgattgaa taagtggaga
      781 acgaatttga aattaatctg tgattgttat ttgtcgggag cataaatatc agggcgtatt
      841 ctttatgttg agaggtgttg gattcttttt tatctgcttt gcaatgttat tggttccctt
      901 cagcagtaga caggaacttc tggaggaggc ggatatatag tttgtgtaag catattaact
      961 ccatatgtta tatattgaag aacttctgct ttacactatc ctaaactgga tcggtttaat
     1021 gtaaaaaaac gatacttttt gggggagggg gcaatttttc cttaaggtat gaaaaaagga
     1081 gctgaagcta tataatagct attaatgcca ctggtatcag tgaatcatgt atataa
//
LOCUS       DAJTMC010000346         1115 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.359, whole genome shotgun sequence.
ACCESSION   DAJTMC010000346 DAJTMC010000000
VERSION     DAJTMC010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1115
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.359"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            1..161
                     /locus_tag="OO130_004300"
                     /pseudo
     CDS             1..161
                     /locus_tag="OO130_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            178..696
                     /locus_tag="OO130_004301"
                     /pseudo
     CDS             178..696
                     /locus_tag="OO130_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          325 a          260 c          244 g          286 t
ORIGIN      
        1 gtgacagact ggcacgtctt cgtaaggagc tgaggctgca ctgtaagcag aaacgcaagt
       61 tcagggcgac cacaaactct gaccacaatc tgccggttac gccgaatctg ctgaatcaga
      121 actttacgcc cacagcacct aatcaggtct gggtcgcaga ttcagttgtc caggccttcc
      181 gaaatcaacc gactgaaggc gctggtcgcg aaactgcagc gtatgcagtt cggtaaaagc
      241 tcagaaaaac ttcgcgcaaa aaccgaacgg cggatacagg aagcacagga gcgaatcagc
      301 gcacttcagg aggaaatggc ggaaacactg ggtgagcaat atgacccggt actgccatct
      361 tccctgcgcc agtcttcagc ccgtaaaccg ttaccagcct cacttccccg tgccccccgg
      421 gtcatccggc cggaagagga atgctgtccg gcctgtggtg gtgaactcag tccactggga
      481 tgtgatgtgt cagagcagct ggagcttatc agcagcgcct ttaaggttat cgaaaaacaa
      541 cgccctaaac tggcctgtcg ccggtgcgac catatcgtgc aggcaccggt gccttcaaaa
      601 cccattgcac gcagttatgc cggagcgggg cttctggccc atgttgttac cggaaagtat
      661 gcagaccatc tgccattata ccgccaaagt gatctactat tccacacagc tatttagctc
      721 tctttttgtt gagctcccat gattctttca tttattgtta ctaattattt tatgttatca
      781 tgatgttaga acacgctctg ctctgtgtct ctattttatc tatcatattc tgaggattaa
      841 ctacataaca aggcgcaaga tatatatcca aaaacaaata ataccgttgc aataattcct
      901 ttcagtaaag gaggtgtgat taatttcagg ggatttcaga gtgaaaattg tacaacatat
      961 tgaaatatgt tcatttcgtt ataatacaca caagaaaata ttaagttaga taatatattc
     1021 taaataacca tagataaaaa tgcacataat ggggaatatt ttatgaaaat acctgaagca
     1081 gtaaatcata ttaatgttca gaataatatt gatct
//
LOCUS       DAJTMC010000347         1091 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.360, whole genome shotgun sequence.
ACCESSION   DAJTMC010000347 DAJTMC010000000
VERSION     DAJTMC010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.360"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            <1..>1091
                     /locus_tag="OO130_004302"
     CDS             <1..>1091
                     /locus_tag="OO130_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCR5367440.1"
                     /translation="YATDPAGNRLPDPELHPDSTLSMWPDNRIARDAHYLYRYDRHGR
                     LTEKTDLIPEGGIRTDDERTHWYHYDSQHRLVHYTRTQYAEPLVESRYLYDPLGRRVA
                     KRVWRRERDLTGWMSLSRKPEVTWYGWDGDRLTTIQNDRTRIQTVYQPGSFTPLIRVE
                     TATGELAKTQRRSLADALQQSGGEDGGSVVFPPVLVQMLDRLESEILADRVSEESRRW
                     LASCGLTVAQMKNQMDPVYTPARKIHLYHCDHRGLPLVLISTEGATEWCAEYDEWGNL
                     LNEENPHHLQQLIRLPGQQYDEESGLYYNRHRYYDPLQGRYITQDPIGLKGGWNFYQY
                     PLSPVNSMDPLGLYEFKSKNIDDIGIFAL"
BASE COUNT          261 a          302 c          336 g          192 t
ORIGIN      
        1 gtatgccaca gacccggcag gtaaccgcct gcccgacccg gagctgcacc cggacagcac
       61 cctcagcatg tggccggata accgtatcgc ccgtgacgcg cactatcttt accggtatga
      121 ccgtcacggc aggctgacag agaaaaccga cctcatcccg gaagggggta tccgcacgga
      181 tgatgagcgc acccactggt accattacga cagtcagcac cggctggtgc actacacgcg
      241 gacacaatat gcagagccgc tggtcgaaag tcgctatctt tacgacccgc tgggccgcag
      301 ggtggcaaaa cgggtatggc ggcgtgaacg ggacctgacg ggctggatgt cgctgtcacg
      361 gaaaccggaa gtgacctggt acggctggga cggcgaccgg ctgaccacaa tacagaacga
      421 cagaacccgc atccagacgg tttatcagcc gggaagcttc acgccactca tcagagttga
      481 aaccgccacc ggtgagctgg cgaaaacgca gcgccgcagc ctggcggatg cccttcagca
      541 gtccggcggc gaagacggtg gcagtgtggt gttcccgccg gtgctggtgc agatgctcga
      601 ccggctggaa agtgaaatcc tggctgaccg ggtgagtgag gaaagccgcc gctggctggc
      661 atcgtgcggc ctgaccgtgg cgcagatgaa aaaccagatg gacccggtgt acacgccggc
      721 gcgaaaaatt cacctgtacc actgcgacca tcgcggcctg ccgctggtgc ttatcagcac
      781 ggaaggggca acagaatggt gcgcagaata cgatgagtgg ggcaacctgc tgaatgaaga
      841 gaacccgcat catctgcagc agctcattcg cctgccgggg cagcagtatg atgaggagtc
      901 cggcctgtat tacaaccgcc accgctatta tgacccgctg caggggcgat atatcactca
      961 ggatccgatt gggctgaagg ggggatggaa tttttatcag tatccgctga gcccggtgaa
     1021 tagcatggat ccattaggat tatatgaatt taaatcaaaa aatatagatg atattggaat
     1081 atttgcattg g
//
LOCUS       DAJTMC010000348         1089 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.361, whole genome shotgun sequence.
ACCESSION   DAJTMC010000348 DAJTMC010000000
VERSION     DAJTMC010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1089
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.361"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(11..322)
                     /locus_tag="OO130_004303"
     CDS             complement(11..322)
                     /locus_tag="OO130_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HCR5367441.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            485..581
                     /gene="ffs"
                     /locus_tag="OO130_004304"
     ncRNA           485..581
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="OO130_004304"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            701..1054
                     /locus_tag="OO130_004305"
     CDS             701..1054
                     /locus_tag="OO130_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HCR5367442.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
BASE COUNT          245 a          274 c          315 g          255 t
ORIGIN      
        1 ccggagaggg ttagtagttc cagcgataac gctgcaagtc gatttgcccg cttcccgata
       61 ccatcacacc ttctgccagt aatgcctgtc gctgacgctg taaatccggt ccggttagcg
      121 aaattgtgcc gtggcgatta accacccggt gccagggtaa ggtgctgcct tcagggagac
      181 gctttaacac accgcccacc tggcgcgcgg cgcggggcga tcccgccagt ttcgccacat
      241 caccgtaagt ggtgacatag ccttcgggaa tagcggcgac gatttgccag acgcgttggg
      301 gaaatgaatc ttctttttcc atcttttctt cctgaggtaa tttttcagca taatctggaa
      361 aaacgcccga gtgaagtcgc attgcgcaag aaaccagcat ctggcacgcg atgggttgca
      421 attagccggg gcagcagtga taatgtgcct gcgcgttggt tctcaacgct ctcaatgggg
      481 gctctgttgg ttctcccgca acgctactct gtttaccagg tcaggtccgg aaggaagcag
      541 ccaaggcaga tgacgcgtgt gccgggatgt agctggcagg gcccccaccc atttctgcct
      601 cccaccgttt cgtcaaaaaa acccaacatg gctaaacttt aaccacaact gacgtcgcaa
      661 gaattgtctg gctgcgcagt acgcttcgga ggtatgtctg atgaagtatg ttgatggttt
      721 tgtggttgcc gttcctgccg ataaaaagga tgcctatcgg gaaatggccg ctaaggctgc
      781 gccattgttt aaagagtttg gcgcgcttcg tattgtcgaa tgctgggcca gcgatgtacc
      841 ggatggcaaa gtgaccgatt ttcgtatggc ggtgaaagcg gaagagaatg aagaggttgt
      901 ctttagctgg attgaatacc cttcaaaaga ggtccgcgac gctgctaatc aaaagatgat
      961 gtcggaccca cggatgaaag agttcggcga gtccatgccg tttgacggca agcgaatgat
     1021 ctatggcgga ttcgagtcaa tcatcgacga ataggtagcg tgacgggctg tgcggcgtgc
     1081 cgtcagccc
//
LOCUS       DAJTMC010000349         1089 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.362, whole genome shotgun sequence.
ACCESSION   DAJTMC010000349 DAJTMC010000000
VERSION     DAJTMC010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1089
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.362"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            <1..447
                     /locus_tag="OO130_004306"
     CDS             <1..447
                     /locus_tag="OO130_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005166249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5367443.1"
                     /translation="LPISAADKRRFYYYFQEKNTPNIERFFVFDSSEYRYALNMREVV
                     FHQFLSDGLRPIVDEDDYFNVHITLVNGGPVIPLSVEPDAPQNEETDDIGQLNAFFDA
                     LDCEPETTDRFMITDEDGEDAFIRIGSIAMVRVALDVLEPVEDDDE"
BASE COUNT          280 a          212 c          263 g          334 t
ORIGIN      
        1 ttgcccattt cagcagcgga taagcggagg ttttattatt attttcagga aaaaaacacc
       61 ccaaacatag aacgcttctt cgtttttgat tcttccgagt atcgttatgc gttaaatatg
      121 cgtgaagtgg tatttcatca gttcctttct gatggtctgc ggccaatcgt tgacgaagac
      181 gattatttta atgtccatat cacgctggta aacggcggac cagtgattcc actgagtgtt
      241 gagccagatg cgccgcaaaa cgaagagacg gatgatatcg gccagttgaa tgcatttttt
      301 gacgcgctgg attgcgagcc tgaaaccacg gatcgtttta tgataaccga cgaagatgga
      361 gaagatgctt tcattcgtat tggttctatc gctatggtgc gagttgcgct tgatgtgctg
      421 gaaccagtgg aagacgatga tgagtagtat tgaatggatt gatttcttaa gaaatggtac
      481 tgagtataca tgtgagtata caaagtaact tcgtattttt aaaaatccat tgatttcata
      541 ttgttgagtc tggaatcgcg gtgcggcctt cgcaccacac cgtatgcaaa tcgagtcgct
      601 caagagcggc tttttttgtg tctgaaatca cccatatccg tgcagtatat aggcctgcca
      661 gaaaagcagt tcagtattct tcattttgta gtcgatggtc ccaagtttgc tctggctgga
      721 agctgcatcg tccacattac gtgggcaggg tggtttcgct atgctacttg aacgttttga
      781 ccatgtggct caggtagcta ataccaaaag acgtcatttt gtctccacgc ccaccttggt
      841 gaacattcaa taaatagttt cggtcgagag cagttatgta gggctagcag acgagatgcg
      901 cagacatctt gttgaagaca attttactga ccacctgaga acgctattct accgaataat
      961 atttattatc ttggcgaata atgatgatta cctgtgtaac tatggtttta tgctgatggg
     1021 accggcagta caatttctgt agtgtactcg caatttcagg tctctggcgt ttattctggt
     1081 gcaaaggag
//
LOCUS       DAJTMC010000350         1057 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.363, whole genome shotgun sequence.
ACCESSION   DAJTMC010000350 DAJTMC010000000
VERSION     DAJTMC010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1057
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.363"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            1..1057
                     /locus_tag="OO130_004307"
                     /pseudo
     CDS             1..1057
                     /locus_tag="OO130_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558238.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    243..359
                     /locus_tag="OO130_004307"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
BASE COUNT          249 a          254 c          325 g          229 t
ORIGIN      
        1 agggcgattc gtatggttct ggaaagtcag ggcgaatatg actcacagtg ggcggcaatt
       61 tgttccattg ccccaaagat tggctgtaca ccggagactc tgcgtgtctg ggtacgccag
      121 catgagcggg ataccggagg cggtgatggc gggctcacca ccgctgaacg tcagcgtctg
      181 aaagagctgg aacgtgaaaa tcgtgaactg cgccgcagta acgatatcct tcgccaggct
      241 tccgcttatt ttgcgaaggc ggagttcgac cgcctctgga aaaagtgatg ccactgctgg
      301 ataagctgcg taagctgtac ggggtcggac cggtatgcag tgaactacat attgccccgt
      361 caacgtatta ccactgtcag caacagcgac atcatcctga taaacgcagt gcccgtgcgc
      421 agcgcgatga ctggctgaag aaagagatac tgcgcgtata cgatgggaat catcaggtat
      481 acggtgtgcg taaagtctgg cgtcagttgt tacgggaagg tatcagagtg gccagatgca
      541 ctgtggcacg tctcatggcg gttatgggac ttgccggtgt tctccggggt aaaaaggtcc
      601 gtacgaccat cagccggaaa gccgttgccg caggcgaccg cgtaaaccgt cagttcgtgg
      661 cagaacgacc tgaccagctg tgggtggctg attttactta cgtcagcaca tggcagggct
      721 tcgtctatgt ggcgttcatt attgatgtgt ttgccggata catcgtgggg tggcgggtct
      781 catcgtccat ggaaacgaca ttcgtgctgg atgctctgga gcaggcgtta tgggcccgtc
      841 gaccgtccgg cacggtccat cacagtgata aaggttctca gtatgtatcg ctggcctaca
      901 cacagcggct taaggaagcc ggattactgg catcaacagg aagtacaggc gactcgtatg
      961 acaacgcgat ggcggagagc atcaatggtc tttacaaagc ggaggtaata caccgtaaga
     1021 gctggaaaaa ccgtgcagaa gtggaactgg ccacact
//
LOCUS       DAJTMC010000351         1051 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.364, whole genome shotgun sequence.
ACCESSION   DAJTMC010000351 DAJTMC010000000
VERSION     DAJTMC010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.364"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            265..1051
                     /locus_tag="OO130_004308"
                     /pseudo
     CDS             265..1051
                     /locus_tag="OO130_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226013.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT          309 a          240 c          246 g          256 t
ORIGIN      
        1 tacccttttg cttttatatc tgcgtctaaa ataaaaatga gccgcaggtt aaccgcaaaa
       61 gttacatgct cacatagcaa aaagaatagc caacttcatt atggcttcag tgagatgtat
      121 ggtcgtagga tttcatacat tgacactggt tatacataca gtaaaaatac tctctactgg
      181 agggcatttt ttatggcagt acgaaaactc accactggga aatggctttg cgaatgttac
      241 cccgccggac gaagtgggcg tcgtgtgcgt aaacaattcg ccaccaaagg cgaagcactg
      301 gcttttgagc gccacacgat ggaagaaacc gaagcaaagc cctggctggg tgaatcagtg
      361 gatcgtcgaa cactgaaaga cgtggttgag ctatggttca aactacatgg taaatctctg
      421 acagctgggc agcatgtcta tgacaaattg ctgttgatgg ttgacgctct gggcaatcct
      481 cttgcaaccg atctcacctc taaaatgttt gcccactatc gagataaacg cctgacaggc
      541 gggatctact tcagcgagaa atggaagaaa ggagcaagcc cggtcaccat taacctggag
      601 caaagctatc taagtagtgt ttttagcgaa ctatcccgtc tgggcgaatg gtcgtatccg
      661 aacccactgg agaacatgcg aaaattcacc atcgcagaaa aagagatggc atggcttacc
      721 catgagcaga ttgttgaatt gctggctgat tgcaaacgtc aggacccaat tctggcactg
      781 gtagttaaga tatgcttaag cacaggcgca cgctagcgtg aagccgtaaa tcttacccgc
      841 tcacaggtga ccaaataccg aattaccttt gtcagaacga aggggaagaa aaacagaagc
      901 atccctatca gtaaagagct ttacgaagag atcatggcgc ttgatgggtt caatttcttc
      961 acagactgct attttcaatt tttatccgtg atggaaaaaa cgtctatcgt gctccctcgc
     1021 ggtcaactca cacacgttct gcgccatacg t
//
LOCUS       DAJTMC010000352         1028 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.365, whole genome shotgun sequence.
ACCESSION   DAJTMC010000352 DAJTMC010000000
VERSION     DAJTMC010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1028
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.365"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(<1..695)
                     /locus_tag="OO130_004309"
     CDS             complement(<1..695)
                     /locus_tag="OO130_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR5367444.1"
                     /translation="MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGE
                     NRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALK
                     ELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMS
                     LELLDAARNALEVIPDFPERDDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQL
                     SPRIMQTLLQQTA"
BASE COUNT          302 a          171 c          220 g          335 t
ORIGIN      
        1 tgggcggttt gttgtaacaa agtttgcatt attctgggag atagttgatt ttctctaaga
       61 ttgacaacgc tatcagaact gaggccaagt atgctttccg gaattgccgt gatccggttc
      121 tgattaagcc agaatattct tataatatga tcatctcttt ctggaaaatc tggtattact
      181 tccaaagcat ttctggctgc atcaagtaat tccaatgaca ttggtaacct cggaagtaca
      241 gacaggtgat tgtcacttac gttaatatat tctagtgacg caggtaattc aggaagtgcg
      301 aacaaatggt tatcactcac atttatatat tccagcgaca ctggtaattc aggaagtgca
      361 gataattgat tactgcttgc attcagttct ttcaatgccc ttggtagctc ggggagcatt
      421 gatagttggt tattgcttac attaatttca tcaagattgt caggcaatcg tggtagagat
      481 ctgagaccta aacaagataa gtccaacgat gtttcactgt tctccagaca taattggagc
      541 cgggtaaaag cagtttccct gttttctccg gaaatgctgt ttttagtcca ttcaacccat
      601 tcggagagat aattattgtg aacattgtcg attgatgtag ttctgtaaaa agagacgttt
      661 ccagtggata gggggggatt atttacagga agcataataa cctcgcagaa ggatatcctg
      721 ataaaatgtg atactgggaa ataataagct aaaataaaat gtttcaggat tgaaatttat
      781 tgttttcatt ttaaggaagt gatgttgatt taactgtaaa aaaacgatac tattttctta
      841 gatggtaaat gtctcgcctg tgctatattt tgtttttcgg ttagttaaat atcgtacgtt
      901 catatttgaa tgttctgccg gaatgcatta tcaatagagg taaagtcgca accccaaatc
      961 gtaaaggaaa ccgtagcacg tcttaagcaa gaacgtgctg cggttggatg ctattttttc
     1021 cctgaagc
//
LOCUS       DAJTMC010000353         1027 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.366, whole genome shotgun sequence.
ACCESSION   DAJTMC010000353 DAJTMC010000000
VERSION     DAJTMC010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1027
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.366"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            <1..442
                     /locus_tag="OO130_004310"
     CDS             <1..442
                     /locus_tag="OO130_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HCR5367445.1"
                     /translation="GVYTEMKEDDVGLYVRGRLLIDDDPLAKRAHAHMKAGSLTGLSI
                     GYVLKDWEYDRTKEAFLLKEIDLWEVSLVTFPSNDEARISDVKNALARGEIPEQKKIE
                     RVLRDVGLSRTQAKAFMAGGYGALSLRDAEDVSSALNALKNLNF"
     gene            457..>1027
                     /locus_tag="OO130_004311"
     CDS             457..>1027
                     /locus_tag="OO130_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HCR5367446.1"
                     /translation="MAVDIKDVEQVAQELQQKFDDFKAKNDKRVDAIEQEKGKLAGQV
                     ETLNGKLSELENLKSDLEKELLELKRPAGGAQNKLATEHKEAFVGFLRKGRGDGLRDL
                     ERKALQVGTDEDGGYAVPEALDRNILTLLKDEVVMRQEASVITVGGSDYKKLVNLGGT
                     ASGWVGETDARSQTATSKLGLIEPFMGEIY"
BASE COUNT          275 a          248 c          312 g          192 t
ORIGIN      
        1 cggtgtttac accgaaatga aggaagacga tgtcgggctt tacgtcaggg gacggttgct
       61 tattgatgat gatcccctcg caaaacgcgc acatgcacac atgaaggccg gttcgttaac
      121 cggcctttct attgggtacg tcctgaaaga ctgggaatac gaccggacga aagaagcctt
      181 tctgctgaaa gaaatcgacc tctgggaagt cagtctggtg acgttcccgt ctaacgacga
      241 ggcgcggatc agcgacgtca agaacgcgct ggcccgcggg gaaatccccg aacagaaaaa
      301 aatcgaaaga gtcctgcgtg atgtcggact ctcccgtacc caggccaaag cattcatggc
      361 cgggggctat ggcgcactgt ccctgcgcga cgctgaggat gtgagctctg cactgaatgc
      421 actgaaaaat ctgaacttct aatcaggaga aatacgatgg cggtagatat taaagatgtc
      481 gaacaggtcg cgcaggagct gcagcagaag tttgacgact tcaaagcaaa gaacgacaag
      541 cgcgtggatg cgattgagca ggaaaaaggc aagcttgccg ggcaggtgga aaccctgaac
      601 gggaaactca gcgagctgga aaacctcaaa agcgatcttg aaaaagagct gcttgagctg
      661 aaacgtccgg caggtggtgc gcaaaataaa ctggccaccg agcataaaga agcgtttgtg
      721 ggcttcctgc gtaaaggccg tggagatggt ctgcgcgatc tggagcgcaa ggcattacag
      781 gtgggcaccg atgaagacgg cggctatgcc gtgccggaag cactggatcg caacattctc
      841 accctgctga aagatgaagt ggtgatgcgc caggaagcct cggtgatcac cgttggtggt
      901 tccgactaca aaaaactggt gaatctgggc ggcacggctt ccggatgggt tggcgagact
      961 gacgcgcgct cccagactgc cacctcaaaa ctgggcctga ttgaaccttt catgggggaa
     1021 atctacg
//
LOCUS       DAJTMC010000354         1018 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.367, whole genome shotgun sequence.
ACCESSION   DAJTMC010000354 DAJTMC010000000
VERSION     DAJTMC010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1018
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.367"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(<1..767)
                     /gene="ipaH3"
                     /locus_tag="OO130_004312"
     CDS             complement(<1..767)
                     /gene="ipaH3"
                     /locus_tag="OO130_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase IpaH3"
                     /protein_id="HCR5367447.1"
                     /translation="MSIMLPINNNFSLSQNSFYNTISGTYADYFSAWDKWEKQALPGE
                     NRNEAVSLLKECLINQFSELQLNRLNLSSLPDNLPPQITVLEITQNALISLPELPASL
                     EYLDACDNHLSTLPELPASLKHLDVDNNQLTMLPELPALLEYINADNNQLTMLPELPT
                     SLEVLSVRNNQLTFLPELPESLEALDVSTNLLESLPAVPVRNHHSEETEIFFRCRENR
                     ITHIPENILSLDPTCTIILEDNPLSSRIRESLSQQTA"
BASE COUNT          289 a          163 c          249 g          317 t
ORIGIN      
        1 tgggcggttt gttgcgacag agactccctg atccgtgagg acagaggatt gtcttcgagg
       61 atgatagtgc aggtcggatc aaggctaagt atattttccg gaatgtgtgt gatgcgattc
      121 tcgcggcacc ggaaaaatat ctcggtttcc tctgagtgat gatttcttac aggtacggct
      181 ggtaggcttt ccagaagatt agtacttaca tcgagcgctt ccagtgattc aggtaactca
      241 gggagaaatg tcagctggtt atttcttact gagagcactt ccagcgatgt aggtaattca
      301 ggaagcatgg ttagctgatt gttatctgca ttaatatatt ccagcaatgc aggcaattca
      361 ggaagcatgg ttagttggtt gttatctaca tcaagatgtt tcagagatgc gggtaattca
      421 ggaagtgttg acaggtgatt gtcacaggcg tcaaggtatt ccagcgatgc tggcaattct
      481 ggtaatgata ttagggcatt ctgagtaatt tccagaacag tgatttgagg tggtaagttg
      541 tcaggtagcg aggacagatt taaacgattc agttgaagct cactgaactg attgatgaga
      601 cattctttaa gtagggagac cgcttcattc cgattttcac cggggagcgc ttgtttttcc
      661 catttatccc atgctgaaaa gtaatcagca tatgtaccgg aaatagtgtt ataaaaagaa
      721 ttttgggaca atgaaaagtt attatttatc ggtaacatga tggacattca gttaaagccg
      781 atggacaaat ggctttgtta aaagaaataa gtaggagttt ttatgaagtt cattccatga
      841 aatgaacttc agcacttttt tattataaaa ggacaaacaa aattggctgt aaaaaaacga
      901 tactgtttca gttaactgtg aatagcttgc ttgcaccgta ttgttcttgt ttttcggtta
      961 gttaaacatc gtacttcata tttgaacgtt ctgccggaat gcattatcaa tagaggta
//
LOCUS       DAJTMC010000355         1009 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.368, whole genome shotgun sequence.
ACCESSION   DAJTMC010000355 DAJTMC010000000
VERSION     DAJTMC010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1009
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.368"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            201..395
                     /gene="bfd"
                     /locus_tag="OO130_004313"
     CDS             201..395
                     /gene="bfd"
                     /locus_tag="OO130_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HCR5367448.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            468..944
                     /gene="bfr"
                     /locus_tag="OO130_004314"
     CDS             468..944
                     /gene="bfr"
                     /locus_tag="OO130_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HCR5367449.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGTPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(941..>1009)
                     /locus_tag="OO130_004315"
     CDS             complement(941..>1009)
                     /locus_tag="OO130_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HCR5367450.1"
                     /translation="PFGPFLAAAGFVIGWDSLLIGR"
BASE COUNT          301 a          205 c          231 g          272 t
ORIGIN      
        1 gctaaagttc tgggttaatt acacgttaat tagttttgaa ttgaaaaggg cgcttcggcg
       61 ccctttttgc tgcccgtaat ttgagctcga aatatttagt cgtaattttg ctcgctgaac
      121 tatggcgcaa ataaaactgt gctattgtaa tcataatcat tctcatttac actttgcgca
      181 gaatttgaac gggagcaatc atgtacgttt gtttgtgtaa tggtgtaagt gacaaaaaaa
      241 ttcgccaggc agtccgtcag tttcatccgc aatctttcca acaattgcgt aagtttattc
      301 ctgtgggaaa tcaatgcggt aagtgcattc gcgccgcgcg cgaagtgatg caggacgagt
      361 tgatgcagat gccagaattt aaagagattg cctgaagacc acgcttattt tttgacatcc
      421 cgctagcccc atctacgctc taatagtgga agcggaggga ctataaaatg aaaggtgatg
      481 ttaaaatcat aaattatctc aataaactat tgggaaatga gcttgtcgca atcaatcagt
      541 attttctcca tgcccggatg tttaaaaact ggggcctgat gcgtctcaat gacgtcgaat
      601 accatgaatc catcgatgag atgaaacacg ctgataaata tatcgagcgt attttgtttt
      661 tagaaggaac ccccaactta caggatttag gcaaactggg gattggtgaa gatgtcgagg
      721 agatgctgca atccgacctg cgattagaac tggaaggcgc gaaggatctg cgtgaggcta
      781 tcgcatatgc cgacagcgtt catgactatg ttagccgcga tatgatgatc gagatccttg
      841 ctgatgaaga aggtcatatc gactggctcg aaactgaact ggatctcatc ggtaaaattg
      901 gcctacaaaa ttacctgcaa tcacaaatta aagttaaaga ttaacggcct atcagcaaac
      961 tatcccagcc tataacgaaa cccgcagccg ccagaaatgg cccaaaagg
//
LOCUS       DAJTMC010000356          980 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.369, whole genome shotgun sequence.
ACCESSION   DAJTMC010000356 DAJTMC010000000
VERSION     DAJTMC010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..980
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.369"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            <1..>980
                     /locus_tag="OO130_004316"
     CDS             <1..>980
                     /locus_tag="OO130_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCR5367451.1"
                     /translation="ELWDHGIAGAFLDYNLYASHYAPHQGDNSQNISSYGQAGVNLGA
                     WRLRTDYQYDQSFNNGKSQANNLDFPRIYLFRPIPAINAKLTIGQYDTESSIFDSFHF
                     SGVSLKSDENMLPPDLRGYAPQITGVAQTNAKVTVSQNNRIIYQENVPPGPFAITNLF
                     NTLQGQLDVKVEEEDGQVTQWQVASNSIPYLTRKGQIRYTTAMGKPTSVGGDSLQQPF
                     FWTGEFSWGWLNNVSLHGGSVLTNRDYQSLAAGVGFNLNSLGSLSFDVTRSDTQLHNQ
                     DKETGYSYRANYSKRFESTGSQLTFAGYRFSDKNFVTMNEYINDTNHYTN"
BASE COUNT          269 a          218 c          203 g          290 t
ORIGIN      
        1 ctgaactttg ggatcatggt attgctgggg catttcttga ctacaattta tatgcctctc
       61 attatgcacc acatcagggc gataattcac aaaatataag ttcctatggg caggctgggg
      121 ttaatcttgg ggcctggcgc ctgcgtactg attaccagta cgatcagtca tttaacaatg
      181 gcaaaagcca ggcgaacaac ctggattttc cgcgtattta tttgtttcgc ccaatcccag
      241 caattaatgc aaaactaact ataggtcaat acgatactga atcctctatt ttcgactctt
      301 tccatttttc tggcgtttcg ttgaaaagcg atgagaatat gttgccgcct gatctacgtg
      361 gttacgcacc gcaaatcacg ggtgttgcac aaacgaatgc aaaggtcact gtctcacaga
      421 acaaccgtat tatttatcaa gaaaatgttc ctcctggccc atttgctatt actaatttat
      481 tcaatacatt acaggggcaa ctggacgtca aggttgaaga agaggacggg caagtcacgc
      541 aatggcaagt tgcatctaat agtattcctt atctgacgcg taaagggcag attcgctaca
      601 ccactgctat gggtaaaccg accagcgttg gtggtgattc cttacaacaa cccttcttct
      661 ggactggtga attctcatgg ggttggctga acaatgtatc cctgcatggt ggttcagttt
      721 taacaaaccg tgattatcaa tctctggctg ccggcgttgg ctttaacctt aactcattgg
      781 gttcattatc ttttgatgtc acacgatctg atactcagtt gcataatcag gataaagaaa
      841 cgggttatag ctaccgcgct aactattcaa aacgttttga atctaccggt agccagctca
      901 ctttcgctgg ttaccgtttc tctgataaaa actttgtgac aatgaatgaa tatatcaatg
      961 acactaacca ttacacgaat
//
LOCUS       DAJTMC010000357          974 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.370, whole genome shotgun sequence.
ACCESSION   DAJTMC010000357 DAJTMC010000000
VERSION     DAJTMC010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..974
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.370"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(<1..804)
                     /locus_tag="OO130_004317"
     CDS             complement(<1..804)
                     /locus_tag="OO130_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5367452.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALARQNENHE
                     VGNKYIPYGKMASWLVEWKNATETQWLKDAPSQPLQQSLKDLERAYKNFFQNRAAFPR
                     FKKRGQNDVFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWY
                     ISIQTESEVSTPVHPSASMVGLDAGVAKLATLSDGTAFEPVNSFQKNQKKLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCIANIRRDYLHKVTTTVSKNHAMIVIED"
     gene            812..>974
                     /locus_tag="OO130_004318"
     CDS             812..>974
                     /locus_tag="OO130_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5367453.1"
                     /translation="MILWSVKKETDIRRGRHCVFLMHVHLVFVTKYRRQIFDHDATEK
                     LRTYFSNVCA"
BASE COUNT          220 a          237 c          232 g          285 t
ORIGIN      
        1 atcctcaatg actatcattg cgtggttttt gctgacggtc gttgtgactt tgtgaaggta
       61 gtccctgcgg atatttgcga tacaggaatg cagtcgctgt attttgcgtt tctgcttctg
      121 ccagttgttg ctgaatttga ccttgcggct taactgtcgc tgaagtctcg ccagcttctt
      181 ctggtttttc tgaaaactgt ttacaggctc aaaggctgtg ccatctgaca gcgtggcgag
      241 tttagccacg ccagcatcca gcccgaccat tgatgctgaa gggtgaaccg gagttgatac
      301 ttcactttct gtctgaatac tgatgtacca cttaccgcag gactggctga cagtgacatt
      361 tttcacaaca cccgtgacct gacggctgtt ccggtagcgc atccagccca gtttcggcag
      421 aaaaatacgg ctgttttcct gatcgagctt aacgccctgc gggtagcgga atacatcatt
      481 ctgtccccgc tttttgaatc ggggaaaagc agcccgattc tggaagaagt ttttgtaagc
      541 ccgctcaagg tctttcagtg actgttgcaa tggctgtgac ggggcatctt taagccattg
      601 cgtttcagtg gcatttttcc actcaaccag ccaggaagcc attttcccgt aagggatgta
      661 tttattaccg acctcatgat tctcattctg acgtgccaga gcacgattga aaacgaaacg
      721 acatgcgcct gcgaagcgcc tcatttcacg ctcctgttga ccaccgggtc ttaactggaa
      781 tttaaatgct tgtagtcgtt tcacaggcca aatgatactt tggtctgtga aaaaagaaac
      841 cgatatccgg cgtggaaggc attgtgtttt cctgatgcat gttcacctgg tctttgtcac
      901 caaataccga cgtcagattt ttgaccatga cgcgacagaa aaactacgca cttacttttc
      961 aaatgtatgt gctg
//
LOCUS       DAJTMC010000358          947 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.371, whole genome shotgun sequence.
ACCESSION   DAJTMC010000358 DAJTMC010000000
VERSION     DAJTMC010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..947
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.371"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            <1..181
                     /gene="tnpB"
                     /locus_tag="OO130_004319"
     CDS             <1..181
                     /gene="tnpB"
                     /locus_tag="OO130_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR5367454.1"
                     /translation="LWADRDGLCLFAKRLERGRFVWPVTREGKVHLTPAQLSMLLEGI
                     AWQHPKRTERPGIRI"
     gene            201..>947
                     /locus_tag="OO130_004320"
     CDS             201..>947
                     /locus_tag="OO130_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISSfl3 family transposase"
                     /protein_id="HCR5367455.1"
                     /translation="MNNELPDDIELLKAMLRKQQSRLRQYACQVAGYEQEIERLKAQL
                     DRLRRMLFGQSSEKKRHKLENQIRQAEKRLSELENRLNTARNLLEDASSVTDSPDTSP
                     PSENPIASKPESPGRKSSRKPLPAELPRETHRLLPAETSCPACGGVLKEMGETISEQL
                     DIINTAFKVIETIRPKLACSRCDVIVQAPLPPKPIERGYASAGLLARILVSKYMEHIP
                     LYRQSEIYARQGVELSRNTMVRWVSEMADKL"
BASE COUNT          262 a          249 c          251 g          185 t
ORIGIN      
        1 actgtgggcc gatcgtgacg ggttatgcct gttcgccaaa cgcctggaac ggggccgctt
       61 cgtctggccg gtgacccggg aagggaaagt gcacctgacg ccagctcagt tatccatgct
      121 actggagggg atcgcgtggc aacatcccaa acggacagaa cggcctggca tccggatata
      181 acccgtgata aaacaaggga atgaacaatg aactccccga tgatattgag ctgcttaaag
      241 ccatgttgcg taagcaacag agtcggcttc gacagtatgc ctgtcaggtc gcgggctatg
      301 agcaggaaat tgaacggctg aaagcgcaac tcgacaggtt gcgtcgtatg ctgttcggcc
      361 agagttcaga gaaaaagcgt cataagcttg aaaatcagat ccgacaggca gaaaaacgac
      421 tgtcggaact ggaaaaccgg ctgaacacag ccagaaatct tctggaagat gcatcgtcag
      481 tcacagattc acctgacacc agtcccccgt cagaaaaccc gatcgccagt aagcctgaat
      541 ccccgggacg aaaatcttca cgaaaaccgc tgccggcaga acttccccgg gagacacatc
      601 gccttctgcc tgctgaaacc agttgcccgg cctgtggagg tgttctgaaa gaaatggggg
      661 aaacaatctc agagcaactg gatatcatta ataccgcctt taaagttatc gaaaccatac
      721 gtcccaaact ggcctgtagc cggtgtgatg tcatcgttca ggcaccactt ccccctaaac
      781 cgatcgaacg cggttatgcc agtgcagggt tacttgcacg gatcctggtc agcaaatata
      841 tggaacatat ccctttatat cgccagtcag aaatatacgc gcgacagggc gtggagctga
      901 gccgtaatac catggtgcgc tgggtatcag aaatggcaga caaactc
//
LOCUS       DAJTMC010000359          944 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.372, whole genome shotgun sequence.
ACCESSION   DAJTMC010000359 DAJTMC010000000
VERSION     DAJTMC010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..944
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.372"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            98..736
                     /gene="ytfB"
                     /locus_tag="OO130_004321"
     CDS             98..736
                     /gene="ytfB"
                     /locus_tag="OO130_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HCR5367456.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNDPDQVAPVAPEPIQ
                     EGQPEEQPQTTQTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GAGKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            complement(720..>944)
                     /locus_tag="OO130_004322"
     CDS             complement(720..>944)
                     /locus_tag="OO130_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR5367457.1"
                     /translation="EGKKHNIFKPDIDPLQVNINIAALGGYYLINQHTLGLVYHISMV
                     SPQALEARRKVIKETILSWLLVDPSSTAHE"
BASE COUNT          252 a          240 c          250 g          202 t
ORIGIN      
        1 gtatgaatat aataaaggcg ggtttaccac ggacatcata gcgttagctt attcataggc
       61 tatgattgag gaacaagacg cggagcagga ggaaaccatg cccgggcgct ttgaactaaa
      121 accaaccctg gagaaagtct ggcacgcgcc ggacaatttt cgcttcatgg acccgctgcc
      181 gccgatgcat cgtcggggga tcattattgc cgccattgta ttggtggtcg gatttctgct
      241 tccatctgat gatacgccca acgcgccggt ggtgacgcgt gaagcgcagt tagatattca
      301 gtcgcaatca caaccgccga cagaagaaca gctgcgggcg caattagtca caccacaaaa
      361 tgatcctgat caggtggctc cggtcgctcc agaacctata caggaaggcc agccagaaga
      421 gcagccgcaa actacccaaa cgcaaccatt ccagccagat agcggaattg ataatcaatg
      481 gcgctcttac cgtgtcgaac ccggtaaaac gatggcgcag ctgttccgcg accacggact
      541 gcccgctacc gatgtctatg caatggcgca ggtagaaggc gcgggtaagc cgctgagtaa
      601 tttgcaaaac gggcagatgg tgaaaatccg ccagaacgcc agcggcgtgg tgacgggctt
      661 aaccattgat acggggaata atcaacaggt cctgttcacc cgccagccgg atggcagttt
      721 tattcgtgcg cggtagatga agggtcaacc aaaagccagc taaggattgt ctctttgatg
      781 accttacgtc tggcttccag tgcctggggg gaaaccatac tgatatgata aaccaggcca
      841 agcgtatgct ggttgatcaa ataatatccg ccaagcgcag caatattaat attcacctgc
      901 aatgggtcga tatctggctt aaaaatattg tgtttcttcc cttc
//
LOCUS       DAJTMC010000360          942 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.373, whole genome shotgun sequence.
ACCESSION   DAJTMC010000360 DAJTMC010000000
VERSION     DAJTMC010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..942
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.373"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            1..566
                     /gene="envY"
                     /locus_tag="OO130_004323"
                     /pseudo
     CDS             1..566
                     /gene="envY"
                     /locus_tag="OO130_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
     gene            complement(612..688)
                     /locus_tag="OO130_004324"
     tRNA            complement(612..688)
                     /locus_tag="OO130_004324"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(652..654),aa:Arg,seq:tct)
BASE COUNT          261 a          227 c          216 g          238 t
ORIGIN      
        1 atctgttgtt tttaaataag aacttaacct gtgtaaagcc ctagtcgcgg ctggcaaccc
       61 cggttatcgc ttgtcatagc cgtacaccgg aagtgttccg gctagccgcc aaccacagca
      121 agcagcaacc cagcaaaccc tgcgaagcgg agttgacgcg cgcattactt tttaccgtat
      181 tgtctaactt tcttgagcaa tcgcggttta ttgccctact gatgtatatc ttacgcagca
      241 gcgtccgcga cagcgtctgc cgcattattc aaagcgatat tcagcattac tggaatctgc
      301 gaattgtcgc cagttcgcta tgtttaagcc ccagcctgct caaaaagaaa ttaaaaaacg
      361 aaaataccag ctatagccag attgtcacag agtgtcgtat gcgttacgcc gtacagatgt
      421 tattgatgga taacaaaaat atcactcagg tggcgcaatt atgtggctat agcagcacgt
      481 cgtactttat ctctgttttt aaggcgtttt acggcctgac accgttgaat tatctcgcca
      541 aacagcgaca aaaagtgatg tggtgaaggg caaagcggaa acggataaga cgggcataaa
      601 tgaagaagaa atggcgcgcc ctgcaggatt cgaacctgcg gcccacgact tagaaggtcg
      661 ttgctctatc caactgagct aagggcgcgt tgataccgca atgcggtgta atcgcgtgaa
      721 ttatacggtc aacccttgct gagtcaatgg cttttgatct ggttgctgaa caagtgaacg
      781 accgcgtctg attttctgat ttatttcgct atagcggcaa acaaacgcac accgctgcgc
      841 gtctgaatca agaaaacccg tattttcatg tatcaaagta caatttaccg ccctaacgga
      901 aaattgtccg ctcctatgag actggtaact atgaaaccaa cg
//
LOCUS       DAJTMC010000361          942 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.374, whole genome shotgun sequence.
ACCESSION   DAJTMC010000361 DAJTMC010000000
VERSION     DAJTMC010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..942
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.374"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            <1..604
                     /locus_tag="OO130_004325"
     CDS             <1..604
                     /locus_tag="OO130_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309936.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR5367458.1"
                     /translation="DNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRN
                     GWENNVKSIDALPYVEAGSNARTSDISSGEYAVMPLAPMKESDAPNEELRQAWEYYHT
                     PRAQYPTAPGYATLRSLNQIITFDAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRA
                     SSQDKRYHIVEGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            604..942
                     /locus_tag="OO130_004326"
                     /pseudo
     CDS             604..942
                     /locus_tag="OO130_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
BASE COUNT          252 a          212 c          245 g          233 t
ORIGIN      
        1 cgacaatacc cgaattggtg cgatgggaat ttgtgccggt gcaggatata cagccaatgc
       61 tgcaattcag gatcgccgca tcaaggccat cggtaccgtt agtgcggtca atattggttc
      121 gatgttccgt aatggttggg aaaacaatgt gaaatctatt gatgccctgc catacgttga
      181 agcgggttca aacgcccgaa ccagcgatat tagcagcgga gaatatgccg ttatgccact
      241 ggcaccaatg aaggagtccg atgcgccgaa cgaagaactg cgtcaggcct gggagtatta
      301 ccacacccct cgcgcgcagt atccaacagc accgggttac gctactttgc gcagccttaa
      361 ccagattatt acctttgatg cttaccatat ggcggaagtg tacctgactc agccgatgca
      421 gattgtggcg ggcagccagg cagggagcaa atggatgagt gacgatctgt acgatcgagc
      481 atcaagccaa gataaacgct atcacattgt tgaaggcgca aaccatatgg atctttatga
      541 cggcaaagcc tatgtggctg aagcaatttc agtattagct ccgttctttg aggaaactct
      601 gtaatgaaac atattcaaat ccgcaatagt gatatggcct ggcatattgc tgccaatatt
      661 cagtttccac caaattttga tgagtcaaaa cagtatccgg cgattatcag cgtccatcct
      721 tttggcagtt gtaaggagca gacgtctggc aatatctatg gcaaagcatt ggctgaaaaa
      781 ggctatctcg ttctcgctta tgacgccagt ttctagggag aatctggtgg ggagcctcgc
      841 tggatagaag atccgacaca gcgcgtagag gatattagcc gtgttatcga ttatgcagta
      901 actctgccat atgttaatgc tgaacgtatt ggcgtgctgg ga
//
LOCUS       DAJTMC010000362          940 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.375, whole genome shotgun sequence.
ACCESSION   DAJTMC010000362 DAJTMC010000000
VERSION     DAJTMC010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..940
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.375"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(416..718)
                     /gene="hicB"
                     /locus_tag="OO130_004327"
                     /pseudo
     CDS             complement(416..718)
                     /gene="hicB"
                     /locus_tag="OO130_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
     gene            complement(764..918)
                     /locus_tag="OO130_004328"
                     /pseudo
     CDS             complement(764..918)
                     /locus_tag="OO130_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415954.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="mRNA interferase"
BASE COUNT          279 a          204 c          191 g          266 t
ORIGIN      
        1 ggaaatggaa gccacgcaag caccttaaaa tcaccatcat acactaaatc agtaagttgg
       61 cagcattacc acatgcgtct tgaagttatt gaaccaaaat atgattctga atataaacac
      121 actcaatttg catgtgacat ggtagacgca gagtaagagg tgaaatgatg caattcaata
      181 gcatgaagat tactcaaaaa tggtgggatg cgatgaaagc taaccaccaa aacgatatta
      241 attaagtaac attttagata tacagcaccc agctaacagc tcacctttta tggatttcat
      301 tataacccct ttgatctgga aactatgtta attagttgat ttctaagcct tgtccttgtg
      361 atggggcttt ttattgcaaa atttttagtt attttgggtt gactacattc gggttgccaa
      421 ttcgcctggc taactcttgc tgagtgattt ctgactgtaa aaaagcattt aacagcaata
      481 ctttagaggc gacgctcaaa ggtacttcaa taaagtgatc atgactattt aatggcgaag
      541 gtaacgggat aagctcgtta tcttcaaaat aaaaatcaaa tgcggtcagt aaagcatctt
      601 ttgccgcttc catcgcttca gcgacagttt cgccctgcgt caacgcttca gggatatcca
      661 caaaagaaac catataaccg ccttccggcg cgggtgtaag agtgacggga taacgcataa
      721 tttcgactgt ctctcgcatg tcattaacct ttttaattgg cgattaactc aaaccgagtt
      781 gtttcaggat tgctttacgc aggagtgacg cggcatgaca ctgcgcctcc catgaaacct
      841 gagtttcaaa tggttgcggc cattcgctac atcgacgccc tgagattcga gccaacgtct
      901 gaactcgctt tgtttcacat cgcctccctg ccgctgagta
//
LOCUS       DAJTMC010000363          932 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.376, whole genome shotgun sequence.
ACCESSION   DAJTMC010000363 DAJTMC010000000
VERSION     DAJTMC010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..932
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.376"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            1..558
                     /locus_tag="OO130_004329"
     CDS             1..558
                     /locus_tag="OO130_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HCR5367459.1"
                     /translation="MAVSGAPAWASEHQSTLSAGYLQPHTDMPGSDDLKGINVKYRYE
                     FTDTLGLVTSFSYANAEDEQKTHYSDTRWHEDSVRNRWFSVMAGPSVRVNEWFSAYAM
                     AGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDNRHSNTSLAWGAGVQFNPTESVAI
                     DLAYEGSGSGDWRTDGFIVGVGYKF"
     gene            622..>932
                     /locus_tag="OO130_004330"
     CDS             622..>932
                     /locus_tag="OO130_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004981847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCR5367460.1"
                     /translation="MAVKISGVLKDGAGKPVVNCAIELRARRTSPTVVAHVVATCVTD
                     NNGAYVIEAEPGYYEVALHCNGWQPTRVGDIDVAPTDAPGTLNAFLNAPKDGDLRPEV
                     M"
BASE COUNT          202 a          217 c          299 g          214 t
ORIGIN      
        1 ctggctgtat ccggtgcgcc tgcatgggcg tctgaacatc agtccacgct gagcgccggg
       61 tatcttcagc cccatactga tatgccaggc agcgatgacc tgaagggcat taacgtgaaa
      121 taccgttatg agtttacgga cacgttgggg ctggtgacgt cattcagcta tgccaacgct
      181 gaagatgagc aaaaaacgca ttacagcgat acccgctggc atgaggattc cgtgcgtaac
      241 cgctggttca gcgtgatggc ggggccgtct gtgcgcgtga atgaatggtt cagcgcgtat
      301 gcgatggcgg gcgtggctta cagccgtgtg tcgaccttct ccggggatta tctccgcgta
      361 actgacaaca aggggaaaac gcacgatgtg ctgaccggga gtgatgacaa ccgccacagc
      421 aacacgtctc tggcgtgggg ggctggcgtg cagtttaacc cgaccgaatc cgtggccatt
      481 gaccttgctt atgaaggttc cggcagtggc gactggcgca ctgacggttt catcgtgggt
      541 gtcggttata agttctgatt agccaggtaa cacagtgtta tgacagcccg ctggttcagg
      601 cgggcttttt gtggggtgaa tatggcagta aagatttcag gtgtactgaa agatggggcg
      661 ggtaaacctg tcgtaaattg tgcgattgaa ctgcgggcca gaagaaccag tccgaccgtt
      721 gtggcacacg ttgttgccac ttgcgtgacg gacaataacg gtgcttatgt gattgaggct
      781 gagccggggt attacgaggt tgcgcttcac tgtaacggct ggcagccaac ccgtgtcggg
      841 gatattgatg tggcaccgac tgatgcaccg gggacactga acgcgtttct gaatgcacca
      901 aaggatggtg atttacgtcc ggaggtgatg aa
//
LOCUS       DAJTMC010000364          926 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.377, whole genome shotgun sequence.
ACCESSION   DAJTMC010000364 DAJTMC010000000
VERSION     DAJTMC010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..926
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.377"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(<1..602)
                     /gene="ospC4"
                     /locus_tag="OO130_004331"
     CDS             complement(<1..602)
                     /gene="ospC4"
                     /locus_tag="OO130_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC4"
                     /protein_id="HCR5367461.1"
                     /translation="MKIPEAVNHINVQNNIDLVDGKTNPNKATKALQKNILRVTNSSS
                     SGISEKHLDHCANTVKNFLRKSIAAQSYSKMFSQGTSFKSLNLSIEAPSGARSSFRSL
                     EHLDKVSRHYISEIIQKVHPLSSDERHLLSIIINSNFNFRHQSNSNLSNNILNIKSFD
                     KIQSENIQTHKNTYSEDIKEISNHDFVFFGVEISNHQEKLP"
BASE COUNT          275 a          147 c          164 g          340 t
ORIGIN      
        1 gggagttttt cttgatgatt ggaaatttca accccaaaaa aaacaaaatc gtggttagag
       61 atttctttaa tatcctcaga gtatgtattc ttatgtgttt gtatattttc agactgaatt
      121 ttatcaaagg atttaatatt aagaatatta tttgacagat tagagttact ttgatgtctg
      181 aagttaaaat tagaattaat aataatagaa agtaagtgac gttcgtctga agaaagagga
      241 tgaacttttt gtattatctc agagatatag tgcctggaca ccttatcgag atgctcaagt
      301 gaccgaaatg atgaccgtgc tccagatggc gcttcaatag aaagatttag tgatttgaaa
      361 ctggtccctt gagaaaacat tttgctataa ctttgcgctg ctatagactt ccttaaaaag
      421 tttttcacag tattagcaca gtgatctaaa tgtttttcac ttatgccaga agaagatgag
      481 tttgttacgc gcaatatatt tttttgtaat gcttttgttg ctttattcgg attcgttttt
      541 ccatcaacaa gatcaatatt attctgaaca ttaatatgat ttactgcttc aggtattttc
      601 atcccccccc caattatgtg cgtttttatc tgtggttatt tagataacat tatctaactg
      661 gacactttct tgtatgtatt atagctaaat gaacagattt taatatgttg tatggttttc
      721 actctgaaat cccctgaatt taatcacgtt tcctttactg aaggcaattt taaccgtagt
      781 atcaattatt aacccttggg cttgatatcc ctctcctctt ctgggcacag gggatgtcag
      841 attacttatt acagaaacca aaagaattgt aagcgtcatc tcagtgtcgt ctatttcaat
      901 gtctgatgat tggcgatgat agagtc
//
LOCUS       DAJTMC010000365          921 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.378, whole genome shotgun sequence.
ACCESSION   DAJTMC010000365 DAJTMC010000000
VERSION     DAJTMC010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..921
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.378"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(<1..>921)
                     /locus_tag="OO130_004332"
     CDS             complement(<1..>921)
                     /locus_tag="OO130_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 family protein"
                     /protein_id="HCR5367462.1"
                     /translation="RFSYGITGGNVFFSCLVFVFFYFAILMTVSADMPLVGCVLTLSL
                     LVLLIFMAAKGLRYQALMTSLNGVRFSFNCSMKGFWWVTFFLPILMAIGMGTVFFIST
                     KMLHANSSSSVIISVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFKVKLDT
                     AYCIKYAILAFLALLPFLAVAGYIIFEQILNAYDSSVYANDDIENLQQFMEMQRKMII
                     AQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCALVVISG
                     ITGGLAYPLLKIWMIDWQAKNTYLLGDLDDL"
BASE COUNT          354 a          207 c          141 g          219 t
ORIGIN      
        1 aaggtcatcc aaatcgccca gcaaatacgt attttttgcc tgccagtcaa tcatccatat
       61 tttcagcagt ggataagcca gaccgcccgt aatcccggat atcactacca acgcacacat
      121 gcgataaagc ataccgtggt acgttaaagt tgagcgaaaa cgaatacgcc catcattcag
      181 tgacaggtta ctcataaaat gatttcgcaa cgacaccgtt aaataactgg tgctaacagc
      241 aatcccaaaa taatagatta actgcgcgat tatcatttta cgttgcattt ccataaattg
      301 ctgtaaattc tcaatatcat catttgcata cacagaacta tcatacgcat ttaatatttg
      361 ttcgaagata atataaccag caacagcgag aaaaggtaat aaagctaaaa atgcgaggat
      421 ggcatatttt atacaatacg cagtatctaa tttcaccttg aaacgatgta taccgaaact
      481 ggtattgctc cagagaaaac tcattaccag gctatataaa gtaccattaa aaataccaat
      541 ggaaacaata ccaactattg ccatcagaac cacagatata ataacactac ttgaactatt
      601 ggcatgtagc atctttgtcg agataaagaa aacagtcccc atcccaatgg ccattaaaat
      661 cggcaagaaa aaggttaccc accagaaccc tttcatagag caattaaaac taaatcttac
      721 gccgttgaga ctagtcatca aggcctgata acgcagtcct tttgctgcca taaatataag
      781 caaaaccaac agtgacaaag tcaaaacaca gccaacaagc ggcatatctg ctgacactgt
      841 cattaagatt gcgaaataga aaaaaacaaa aacaagacaa ctaaaaaaaa cattcccacc
      901 agtaattcca taagaaaatc g
//
LOCUS       DAJTMC010000366          914 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.379, whole genome shotgun sequence.
ACCESSION   DAJTMC010000366 DAJTMC010000000
VERSION     DAJTMC010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..914
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.379"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            <1..>914
                     /gene="tuf"
                     /locus_tag="OO130_004333"
     CDS             <1..>914
                     /gene="tuf"
                     /locus_tag="OO130_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR5367463.1"
                     /translation="AHVDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHIL
                     LGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPIVRGSALKAL
                     EGDAEWEAKILELAGFLDSYIPEPERAIDKPFLLPIEDVFSISGRGTVVTGRVERGII
                     KVGEEVEIVGIKETQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAK
                     PGTIKPHTKFESEVYILSKDEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMP
                     GDNIKMVVTLIHPIAMDDGLRFAIREGGR"
BASE COUNT          212 a          230 c          264 g          208 t
ORIGIN      
        1 gcacacgtag actgcccggg gcacgccgac tatgttaaaa acatgatcac cggtgctgct
       61 cagatggacg gcgcgatcct ggtagttgct gcgactgacg gcccgatgcc gcagactcgt
      121 gagcacatcc tgctgggtcg tcaggtaggc gttccgtaca tcatcgtgtt cctgaacaaa
      181 tgcgacatgg ttgatgacga agagctgctg gaactggttg aaatggaagt tcgtgaactt
      241 ctgtctcagt acgacttccc gggcgacgac actccgatcg ttcgtggttc tgctctgaaa
      301 gcgctggaag gcgacgcaga gtgggaagcg aaaatcctgg aactggctgg cttcctggat
      361 tcttacattc cggaaccaga gcgtgcgatt gacaagccgt tcctgctgcc gatcgaagac
      421 gtattctcca tctccggtcg tggtaccgtt gttaccggtc gtgtagaacg cggtatcatc
      481 aaagttggtg aagaagttga aatcgttggt atcaaagaga ctcagaagtc tacctgtact
      541 ggcgttgaaa tgttccgcaa actgctggac gaaggccgtg ctggtgagaa cgtaggtgtt
      601 ctgctgcgtg gtatcaaacg tgaagaaatc gaacgtggtc aggtactggc taagccgggc
      661 accatcaagc cgcacaccaa gttcgaatct gaagtgtaca ttctgtccaa agatgaaggc
      721 ggccgtcata ctccgttctt caaaggctac cgtccgcagt tctacttccg tactactgac
      781 gtgactggta ccatcgaact gccggaaggc gtagagatgg taatgccggg cgacaacatc
      841 aaaatggttg ttaccctgat ccacccgatc gcgatggacg acggtctgcg tttcgcaatc
      901 cgtgaaggcg gccg
//
LOCUS       DAJTMC010000367          908 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.380, whole genome shotgun sequence.
ACCESSION   DAJTMC010000367 DAJTMC010000000
VERSION     DAJTMC010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..908
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.380"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(<1..>908)
                     /locus_tag="OO130_004334"
     CDS             complement(<1..>908)
                     /locus_tag="OO130_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5367464.1"
                     /translation="AFLTGIAGALQTPTLSIFLADELKARPIMVGFFFTGSAIMGILV
                     SQFLARHSDKQGDRKLLILLCCLFGVLACTLFAWNRNYFILLSTGVLLSSFASTANPQ
                     MFALAREHADRTGRETVMFSTFLRAQISLAWVIGPPLAYELAMGFSFKVMYLTAAIAF
                     VVCGLIVWLFLPSIQRNIPVVTQPVEILPSTHRKRDTRLLFVVCSMMWAANNLYMINM
                     PLFIIDELHLTDKLAGEMIGIAAGLEIPMMLIAGYYMKRIGKRLLMLIAIVSGMCFYA
                     SVLMATTPAVELELQILNAIFLGILC"
BASE COUNT          280 a          213 c          213 g          202 t
ORIGIN      
        1 cacagagaat accaaggaag atggcattta gaatttgcaa ttccagctca accgccggag
       61 tcgtcgccat gagtacgctg gcgtaaaaac acataccact cacgatagca atgagcatta
      121 atagtcgctt gccaatacgt ttcatgtaat agcctgcgat taacatcatc ggaatttcca
      181 gaccggcagc gataccaatc atttctccag ccagtttatc ggttagatgc agttcatcaa
      241 taataaatag cggcatattt atcatgtaga gattattcgc cgcccacatc attgaacaga
      301 ccacaaaaag tagccgcgta tcccgcttcc tatgggtgga gggtaaaatt tctacgggtt
      361 gggtaacgac aggaatattt ctttgtattg atggcaaaaa caaccagact atcagcccgc
      421 aaacaacaaa tgcgatggca gcggtgagat acatcacttt aaaactaaac cccattgcca
      481 gttcataagc gagcggtggc ccgataaccc aggcaagcga gatctgagca cgtaaaaatg
      541 tactgaacat gaccgtctca cggcctgttc tgtcggcgtg ttcacgggcg agggcgaaca
      601 tttgcgggtt tgcggtagaa gcaaaactac tcagaagtac gcccgtagag aggagaatga
      661 agtagttgcg attccacgca aaaagcgtgc aggccagcac tccaaataag caacatagca
      721 gaatcagtaa tttacggtcg ccttgtttat cggagtgcct tgccagaaat tgactgacca
      781 gaattcccat aatagcgcta ccggtgaaga aaaaacctac cattatagga cgggctttca
      841 gttcatctgc gaggaatata cttagggtag gagtctgaag agcgcccgca atacccgtca
      901 gaaaggcg
//
LOCUS       DAJTMC010000368          880 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.381, whole genome shotgun sequence.
ACCESSION   DAJTMC010000368 DAJTMC010000000
VERSION     DAJTMC010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..880
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.381"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            <1..324
                     /locus_tag="OO130_004335"
     CDS             <1..324
                     /locus_tag="OO130_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000789105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5367465.1"
                     /translation="VNRHLAEVGGITGRTIFISSMSELLKNMALNGYGIAWLPIWSIV
                     DELQTKRLICLDAAKLTVPIQAYIYRMNTRLNRTAENLWRILQEHMPDDLIQQISMEE
                     PARRN"
     gene            complement(442..880)
                     /gene="tnpA"
                     /locus_tag="OO130_004336"
                     /pseudo
     CDS             complement(442..880)
                     /gene="tnpA"
                     /locus_tag="OO130_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459923.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT          220 a          238 c          180 g          242 t
ORIGIN      
        1 gtcaatcgtc atctggcaga ggttggaggt attaccgggc gcacgatatt tatctcttcg
       61 atgagcgagt tattaaagaa tatggcgctc aacggatacg gcattgcctg gttaccgatc
      121 tggtcgatcg tcgatgaact acagaccaaa cgtctgatat gccttgatgc tgctaaattg
      181 acagtcccca ttcaggctta tatttaccga atgaacaccc gactgaatag aaccgccgag
      241 aacctctggc gtattttaca ggaacatatg ccagacgatt taatccagca gatatcaatg
      301 gaagagccag cacgccgcaa ttgacgacgt gcaataaata tcctccggca tagccggagg
      361 tttttcaaat gcgcctataa ggctctctta ccagccgcgc cctaacaggc gcacacgatc
      421 tgacatttgc atccaacttc gttacttacg gtccgtaaac gggctgcccg gacagggaat
      481 cgataactgc tctcccattt tatcctcttc aagctggtgc tttatgtaat cctgtatctt
      541 cgccgtgttc ttacccactg tatcgacgta gtcccccctg caccagaact ccctgttcct
      601 gtatttgaat ttcaaatcac caaactgctc gtaaagcatc agactgcttt tccctttcag
      661 atatcccata aagccggata cgctcatttt gggcgggatc tccacaagca tatggatatg
      721 atctgcacag cattcagctt ccagaatccg tacgcttttc cactcacaca gctttctcaa
      781 aatactgcct gttgctctac gcttctctct gtagaacacc tgtcttcggt attttggcgc
      841 aaaaaactat gtgatattta cagttccatc gggtgtgcgc
//
LOCUS       DAJTMC010000369          865 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.382, whole genome shotgun sequence.
ACCESSION   DAJTMC010000369 DAJTMC010000000
VERSION     DAJTMC010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..865
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.382"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            <1..841
                     /locus_tag="OO130_004337"
     CDS             <1..841
                     /locus_tag="OO130_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCR5367466.1"
                     /translation="LVGYIAWLATAYRMSFSREINLGLAQHEFELFCQPLLNARSQQC
                     IGVEILLRWNNPRQGRISPDVFIPIAEEHHLIVPLTRYVMAETIRQRHVFPMSSQFHV
                     GINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITERDALLDVDYRIARELHRKNVK
                     LAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSDAVNSTVTDIIIALGQRLNIEL
                     VAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFPKWLAGSQPPPARHNGHITPIM
                     PLR"
BASE COUNT          200 a          220 c          222 g          223 t
ORIGIN      
        1 actggtgggc tacatcgcct ggctggcgac cgcttaccgg atgagctttt cccgcgaaat
       61 caatctgggc ctggcgcaac atgagttcga attgttctgt cagcctttgc ttaatgcgcg
      121 cagccagcaa tgtattggtg tagagatttt gctgcgctgg aacaatccgc gtcagggccg
      181 gatttcaccg gatgtgttta ttcctatcgc ggaagaacat catttaattg tgccactgac
      241 ccgctatgtg atggcagaaa ccattcgtca gcgccatgtt ttcccgatga gtagtcagtt
      301 tcatgttggc attaacgtcg cacccagcca ttttcgccgt ggtgtgctga taaaagatct
      361 caatcagtac tggtttagcg ctcacccgat tcagcaactg atcctcgaaa tcaccgaacg
      421 cgatgcctta ctggatgttg attatcggat tgcccgcgag ctacatcgta aaaacgtcaa
      481 actggcgatt gatgacttcg gcaccggcaa cagttcattt tcctggcttg aaacattacg
      541 tcctgacgtg ctgaaaattg ataagtcatt taccgcagct ataggttctg acgcggttaa
      601 ctcgacggtg accgatatca tcatcgcgct ggggcaaaga ctgaatattg aactggtggc
      661 ggagggcgtg gaaacgcaag aacaggcgaa gtatttgcgc cgtcatggcg tgcatatttt
      721 gcaagggtat ttgtacgcac agccgatgcc gctacgcgat tttcccaaat ggctggcggg
      781 cagccaaccg ccgcccgccc ggcataatgg acatatcacg cccattatgc cgttacgtta
      841 acgctgatat gactttccgt gtcaa
//
LOCUS       DAJTMC010000370          860 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.383, whole genome shotgun sequence.
ACCESSION   DAJTMC010000370 DAJTMC010000000
VERSION     DAJTMC010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..860
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.383"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(49..>860)
                     /locus_tag="OO130_004338"
     CDS             complement(49..>860)
                     /locus_tag="OO130_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HCR5367467.1"
                     /translation="MSLSRDDVDVYSRDFSRTSQMNTNSVDVRYRNIPLWDDATLSLM
                     AKYSAPNKTDQQQDNENDNSYFEMKDSWMLTSVLRQNLQRDTFNEFTLQVANNSYASS
                     FASFSDASNTMAHGRYYYGDHTNGIAWRLISQGEMYLTDNIIMANALVYSHGEDVYSY
                     ESGAHSDFDSIRTVIRPAWIWNTWNQTGLELGWFKQQNKTQQGVTLNESAYKTTLWHA
                     LKVGESILGSRPEIRFYGTYINILDNELSNFKFNENSKDEFMAGIQAEVWW"
BASE COUNT          257 a          194 c          152 g          257 t
ORIGIN      
        1 aaaacctttt ttaaatcctg acatacacaa ctccttaact aaaatagatt accaccagac
       61 ttccgcctgg atgccggcca taaactcgtc tttgctgttc tcattaaact taaaattcga
      121 taattcgtta tccagaatat tgatatacgt tccatagaag cgaatttctg gtcgtgaacc
      181 taaaatgctt tcacccactt tcaatgcatg ccagagtgtc gttttataag ccgattcatt
      241 tagtgttaca ccctgctgag ttttgttctg ttgcttaaac cagcctaatt caagccccgt
      301 ctgattccat gtattccaga tccaggccgg tcttattaca gtgcgaatac tgtcaaaatc
      361 actatgagca ccactttcat aactataaac atcttcgccg tgagaataga caagcgcgtt
      421 agccataata atattgtcag taagatacat ctcgccctga gagattaaac gccaggcgat
      481 cccattggta tgatcaccat agtaatagcg accatgcgcc atcgtattac tggcatctga
      541 gaaactggca aaactgctgg cataggaatt attggcaacc tgtaacgtaa attcattaaa
      601 cgtatcgcgt tgcaggtttt gccgtaaaac agaagtcagc atccagctat ctttcatttc
      661 aaaataactg ttgtcatttt cgttatcttg ttgttgatcc gttttattag gtgcggaata
      721 tttagccatt aatgataatg tcgcatcatc ccataacggg atattgcgat aacgaacatc
      781 cacagaattg gtattcatct ggctggtacg cgaaaaatca cgagagtaaa catcgacatc
      841 atcacggctt aaggacatat
//
LOCUS       DAJTMC010000371          850 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.384, whole genome shotgun sequence.
ACCESSION   DAJTMC010000371 DAJTMC010000000
VERSION     DAJTMC010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..850
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.384"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            <1..>850
                     /locus_tag="OO130_004339"
     CDS             <1..>850
                     /locus_tag="OO130_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCR5367468.1"
                     /translation="EHADGSVTQSQFDAVGRLRTQTDAAGRTTEYSPDVVTGLITRIT
                     TPDGRASAFYYNHHSQLTSATGPDGLEIRREYDEWGRLIQETAPDGDIIRYRYDNPHS
                     DLPCATEDATGSRKTMTWSRYGQLLSFTDCSGYVTRYDHDRFGQMTAVHREEGLSQYR
                     AYDSRGQLIAVKDTQGHETRYEYNAAGDLTAVIAPDGSRNGTQYDAWGKAIRTTQGEL
                     TRSMEYDAAGRVIRLTSENGSHTTFRYDVLDRLIQETGFDGRTQRYHHDLTGKLIRSE
                     DEGLVTH"
BASE COUNT          209 a          251 c          254 g          136 t
ORIGIN      
        1 gaacacgcgg acggcagcgt cacgcagagt cagtttgacg ccgtgggcag gctcaggaca
       61 cagacggatg ccgcaggccg gacaacagaa tacagcccgg atgtggtgac gggcctcatc
      121 acgcgcatca ccacgccgga tggcagggca tcggcgtttt actataacca ccacagccag
      181 ttaacgtcag ccaccgggcc tgacgggctg gaaatacgcc gggaatatga tgaatggggc
      241 cgtctgattc aggaaactgc ccctgacggc gatatcatcc gctaccgtta tgataatcca
      301 cacagtgact taccctgcgc aacggaagat gccaccggta gccggaaaac catgacgtgg
      361 agccgttacg gtcagttgct gagcttcacc gactgttccg gttatgtaac ccgttatgac
      421 catgaccgct tcgggcagat gacggcggtg caccgcgagg aagggctgag tcagtaccgc
      481 gcatacgaca gccgtggaca gttaattgcc gtgaaagaca cgcagggcca tgaaacgcgg
      541 tatgaataca acgccgccgg tgacctgacc gccgtcattg ccccggacgg cagcagaaac
      601 gggacacagt acgatgcgtg gggaaaggcc atccgtacca cgcagggcga gctgacgcgc
      661 agtatggaat acgatgctgc aggacgggtc atccgcctga ccagtgaaaa cggcagccac
      721 accaccttcc gttacgatgt actcgaccgg ctgatacagg aaaccggctt tgacggccgc
      781 acacagcgtt atcaccacga cctgaccggc aaacttatcc gcagcgagga tgaggggctg
      841 gtcacccact
//
LOCUS       DAJTMC010000372          833 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.385, whole genome shotgun sequence.
ACCESSION   DAJTMC010000372 DAJTMC010000000
VERSION     DAJTMC010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..833
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.385"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            <1..561
                     /gene="ospC4"
                     /locus_tag="OO130_004340"
     CDS             <1..561
                     /gene="ospC4"
                     /locus_tag="OO130_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC4"
                     /protein_id="HCR5367469.1"
                     /translation="QGFREFCYNKNIDPVSLDRIINFVFQLEYHIPRMLSTDNFKKIK
                     LRDISLEDAIKASNYEEINNKVTDKKMAHQALAYSLGNKKADIALYLLSKFNFTKQDV
                     AEMEKMNNNRYCNLYDVEYLLSKDGANYKVLEYFINNGLVDVNKKFQKANSGDTMLDN
                     AMKSKNPKMIDFLLKNGAILGKRFEI"
BASE COUNT          293 a          134 c          163 g          243 t
ORIGIN      
        1 caaggattca gagagttttg ttataacaaa aatattgatc ctgttagtct ggatagaatt
       61 ataaactttg tgtttcagct tgagtatcat ataccaagaa tgctaagtac agataacttc
      121 aaaaaaataa aactcagaga catatctttg gaggatgcga ttaaagcatc taattatgaa
      181 gagattaaca ataaagtgac tgataaaaaa atggctcatc aggcccttgc ttattcttta
      241 ggcaataaaa aagctgatat agcactttat ttactatcta aatttaattt tacaaaacaa
      301 gatgtcgcag agatggagaa aatgaacaac aatagatatt gtaatctgta tgatgttgaa
      361 tacttactta gtaaagatgg tgccaactat aaagtgctag aatattttat caataatgga
      421 ttggttgatg taaacaaaaa atttcaaaaa gcaaatagtg gggacactat gcttgacaat
      481 gcaatgaaaa gcaaaaatcc aaaaatgatt gattttttat taaaaaatgg agcaatatta
      541 ggcaaacgat ttgagatatg acctaaaagc tcggccaaat aataatgtcg tcgattatcg
      601 aagtcatcat gagtgattct gtccccacag tgcagcatca gcatgacagt gaattgtctg
      661 gcgtggtcca tttaatgaga tggagatggt ccctctttcc taaatagctg tgccgaatac
      721 cgctacactt ttgccagccc atgtttgcct ccggggaatg ggctgacggt tccttcaaaa
      781 tccattgcac gcagttatgt cggagcggga acttgactcc tgtctttacg gga
//
LOCUS       DAJTMC010000373          826 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.386, whole genome shotgun sequence.
ACCESSION   DAJTMC010000373 DAJTMC010000000
VERSION     DAJTMC010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..826
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.386"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(1..761)
                     /gene="istA"
                     /locus_tag="OO130_004341"
                     /pseudo
     CDS             complement(1..761)
                     /gene="istA"
                     /locus_tag="OO130_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
BASE COUNT          198 a          217 c          217 g          194 t
ORIGIN      
        1 cagtgctccc catcgggcgc aggcgagtca ttagtgggat gtaaaaactg ttccatccgt
       61 tccaccttac ctttagtctg tgccctgaag gggcgacaca gtcggggaga gaagcccatc
      121 tccttgccga actgccacag cgaaggatgg aaccggtgct gaccggtctg atatgcgtca
      181 cgttgcagaa ccacagtttt catattgtca tacaacactt cgcgcggcac accaccaaag
      241 aagcggaacg cattacgatg gcaggtctcc agcgtgtcat aacgcatatt gtcagtgaat
      301 tcgatgtaca acattcggct gtatccggga acagcaacga acacgtgaag cggtgagcga
      361 ccattacgca tagtgcccca gtcaacctgc atctgtcgtc cgggttcagt ttcgaaccga
      421 acggcaggct cctgctcctg aggaaccgag agagaacgaa tgaatgccct gagaatggtc
      481 attccgccac gatatccctg gtctctgatc tcgcgagcga ttaccgttgc cgggattttg
      541 taaggatgag catcggcgat gcgttgacga atataatccc ggtattcatc caggagtgaa
      601 gcaacagcag gtcgcggcgt atattttggc ggctcagatt ttgcctgcaa ataacgttta
      661 acggtattgc gggagagccc cagttctctg gcaatcgccc ggctactcat tccctgcttg
      721 tgcaggattt taatttccat aactgtctca aaagtgacca taagctctcc tgaatcagga
      781 gagcagatta ccccctggat ctgatttcag gcgttgggtg tggatc
//
LOCUS       DAJTMC010000374          825 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.387, whole genome shotgun sequence.
ACCESSION   DAJTMC010000374 DAJTMC010000000
VERSION     DAJTMC010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..825
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.387"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            9..800
                     /gene="fdrA"
                     /locus_tag="OO130_004342"
                     /pseudo
     CDS             9..800
                     /gene="fdrA"
                     /locus_tag="OO130_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308607.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
BASE COUNT          198 a          192 c          253 g          182 t
ORIGIN      
        1 gagttgttat gatccacgcc tttattaaaa aagggtgttt tcaggattcg gtcagtttaa
       61 tgattatttc acgaaaactc agcgaatcag aaaatgttga tgatgtttcc gtaatgatgg
      121 gtacgcccgc caataaagcg ttattagata ccacaggttt ctggcatgac gattttaata
      181 acgccacgcc gaacgatatt tgcgtggcaa ttcgtagcga agcggcggat gcggggatcg
      241 cgcaggcgat tatgcagcag cttgaagagg cgctaaaaca actggcgcag ggggcaggca
      301 gcagccagtc cttgacgcag gtgcgtcgct gggacagtgc ctgtcagaaa ttatccgatg
      361 ccaatctggc gctgatttca gtggctggcg agtatgcggc ggagctggcg aatcaggcgc
      421 tggagcgcaa cctcaacgtg atgatgttct ccgataacgt cacgctggaa gatgaaatcc
      481 aacttaaaac ccgcgcgcga gaaaaaggct tgctggtgat ggggccggac tgcggtacgt
      541 cgatgattgc cggcacgccg ctggcttttg ctaacgtgat gccggaaggc aacattggcg
      601 tcattggcgc ttccggtacc gggattcagg aactgtgttc gcagattgcg caggcagggg
      661 agggaattac tcacgcgatt ggtcttggcg agcgcgacct cagccgtgaa gtaggcggca
      721 tcagtgcgct gacggcgctg gaaatgctca gtgcagacga gaaaagcgaa gtgctggcat
      781 ttgtttcaaa gccacctgcc tagactggcc ccctgaatct ccaga
//
LOCUS       DAJTMC010000375          824 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.388, whole genome shotgun sequence.
ACCESSION   DAJTMC010000375 DAJTMC010000000
VERSION     DAJTMC010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..824
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.388"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            <1..>824
                     /locus_tag="OO130_004343"
     CDS             <1..>824
                     /locus_tag="OO130_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66-like element ISSfl3 family transposase"
                     /protein_id="HCR5367470.1"
                     /translation="DYVLEAGKVHADDTPVKVLAPGNGKTKTGRLWVYVRDDRNAGSS
                     LPAAVWFAYSADRKGEHPQLHLAKYQGVLQADAYAGYNVLYETGRVKEAGCLAHARRK
                     IHDEDVRRPTEMTQEALRRIAELYDIEAEIRGSPAEERLAVRKARSVQLMQSLYDWIQ
                     LQRKTLSKHAEMAKAFDYILNHWNALNEFCRDGWVEIDNNIGENALRSVAVGRKNYLF
                     FGSDKGGESAAIIYSLLVTCKQNEVEPEDWLREVIEKLNDWPSNQVHELLPWNFSS"
BASE COUNT          222 a          188 c          259 g          155 t
ORIGIN      
        1 atgactatgt tctggaggca ggaaaggtgc acgcagatga cactccggtg aaagtactgg
       61 ccccggggaa cggaaagacg aaaacgggtc gtctgtgggt atacgtcagg gatgatcgta
      121 atgcgggttc atccctgccg gcagccgtct ggttcgcgta ttcggcagat cgcaaaggag
      181 aacatccgca gctccacctg gcaaagtatc agggcgtact gcaggctgat gcctatgcag
      241 gttataacgt actgtacgaa acgggccggg tgaaggaagc cgggtgcctg gcccacgccc
      301 gccgaaaaat ccatgacgag gatgtgcgcc gtccgacaga aatgactcag gaagcgctca
      361 gacggatagc agagttatac gacatagaag cggagatacg tggcagtccg gcagaggaac
      421 ggcttgcagt cagaaaagcc agaagcgtcc agttgatgca gtcattgtac gactggatac
      481 agttgcagag gaaaacgctg tcgaaacatg cggagatggc gaaggcgttc gactatatcc
      541 tgaatcactg gaatgcgctg aacgagttct gtcgtgacgg ctgggtggaa atagacaaca
      601 acatcggtga aaacgcgtta cgatcggtgg cggttggaag aaaaaattat ctctttttcg
      661 gctcagacaa gggaggagaa agtgcggcga tcatctacag tctgctggtc acctgcaaac
      721 agaacgaagt ggagccggag gactggttgc gcgaagtgat cgagaagctc aatgactggc
      781 cgtcgaacca agtgcatgaa ctgctgccct ggaacttctc gtct
//
LOCUS       DAJTMC010000376          816 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.389, whole genome shotgun sequence.
ACCESSION   DAJTMC010000376 DAJTMC010000000
VERSION     DAJTMC010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..816
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.389"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            122..816
                     /locus_tag="OO130_004344"
                     /pseudo
     CDS             122..816
                     /locus_tag="OO130_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          311 a          146 c          134 g          225 t
ORIGIN      
        1 ggctattgtg cgctcataca ctcaaattaa agataggttc taaataaatg agcgtttttt
       61 gatagtctat ttcattaggt aatatatatt tgtaacaaat caatcaaaat ggaataaaat
      121 catgctacca tctatttcaa tcaacaatac cagcgcaact tacccagaat ccattaatga
      181 aaataacaat gatgaagtta atggattagt acaagagctc aaaaaccttt ttaatggtaa
      241 ggaataagca cctgtattaa acatctactt gagcttataa aaaacgccat acgagtaaac
      301 gacgatcctt atagatttaa tattaataat tcctcagtta cttatattga tattggctcc
      361 aatgatacag accatattac tattggtatc gacaaccaag aaccaataga attacctgcg
      421 aactataaag acaaagaact cgtccgtact atcattaatg acaacatagt tgagaagact
      481 catgatatca ataacaagga aatgatcttc agcgcattaa aagaaatata tgatggagct
      541 cctagtttta tcttcgataa gatatcacac aaactcagac atacggtaac ggaatttgat
      601 gagagcggga aaagcgaacc aacggactta tttacctggt acggtaaaga taaaaaaggc
      661 gactctctcg ctattgtaat taaaaataaa aacggaaatg attacttatc cctcggttac
      721 tacgatcagg acgactacca cattcaaaga ggaattcgta ttaatggtga tagtctcacc
      781 caatattgta gtgaaaacgc caggagtgct tcagcg
//
LOCUS       DAJTMC010000377          811 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.390, whole genome shotgun sequence.
ACCESSION   DAJTMC010000377 DAJTMC010000000
VERSION     DAJTMC010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..811
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.390"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            <1..346
                     /locus_tag="OO130_004345"
     CDS             <1..346
                     /locus_tag="OO130_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5367471.1"
                     /translation="VMCLTKPAFSYYIAAYLKLFIENYYEADALVDTILNMLTPPVRN
                     GIPSTTWIAKNLDCFSRNKRVAISFTFQYLCNEYNDPNTEKALNIYWGQYLNESLLIK
                     NVQNSKNKCKSQ"
     gene            complement(407..586)
                     /locus_tag="OO130_004346"
     CDS             complement(407..586)
                     /locus_tag="OO130_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005006008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5367472.1"
                     /translation="MVAVAAAVTDALLVFFRQLAPFVVLPALGIFIIHGTVRQAVGFI
                     VMPERDQALMSLTVI"
     gene            591..794
                     /locus_tag="OO130_004347"
     CDS             591..794
                     /locus_tag="OO130_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS domain-containing protein"
                     /protein_id="HCR5367473.1"
                     /translation="MMKNQMEPEYTPLRKIHLYHCDHRGLPLALIRSDGRTGWRVEYD
                     EWGNLLSEDNPHRERSSEVHFLY"
BASE COUNT          251 a          169 c          188 g          203 t
ORIGIN      
        1 tgtcatgtgt ctcactaaac cagcattctc atattatatt gcagcctatt taaaactttt
       61 tattgaaaat tattacgaag ctgatgcttt agtggatact attttaaata tgctcactcc
      121 tcctgttagg aatggtatcc ccagcactac atggatagca aaaaatcttg attgctttag
      181 tcgtaataaa agggtggcta tttcttttac attccaatat ttatgtaatg aatataatga
      241 tccaaatact gaaaaggcgt taaatatata ttggggacag tatctgaatg aatcattgct
      301 tattaaaaat gtacagaact ctaaaaataa atgtaaatca cagtaagaca gaaccaagca
      361 gaaatcgtgg gcgtggttcg caccctgacg gagggtgtag cgtgtactag atcacagtga
      421 gcgacatcaa agcttggtca cgttctggca ttacgatgaa gccgaccgcc tgacgcaccg
      481 taccgtgaat gatgaagatg ccgagtgctg gcagtacaac gaacggggct agctgacgga
      541 aaaagacgag cagcgcatcc gtgacggcgg cagccacagc caccattatc atgatgaaaa
      601 accagatgga gccggaatac acgccgctgc gtaaaattca cctgtaccac tgcgaccacc
      661 ggggactgcc gctggcgctg atacgcagtg acggccgaac cggatggcgt gtagagtatg
      721 acgaatgggg caacctgctg agtgaggata acccgcacag ggaaagatca tcagaagttc
      781 actttttgta ctaaataatt cgcattttat g
//
LOCUS       DAJTMC010000378          774 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.391, whole genome shotgun sequence.
ACCESSION   DAJTMC010000378 DAJTMC010000000
VERSION     DAJTMC010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..774
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.391"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            52..774
                     /locus_tag="OO130_004348"
                     /pseudo
     CDS             52..774
                     /locus_tag="OO130_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          227 a          189 c          206 g          152 t
ORIGIN      
        1 gccacctgaa cagaggtgat atgctcacct cagaacaaca caggtgctcc aatgaaaaaa
       61 agaaatttta gcgcagagtt taaacgcgaa tccgctcaac tggttgttga ccagaaatac
      121 acggtggcag atgccgccaa agctatggat gttggccttt ccacaatgac aagatgggtc
      181 aaacaactgc gtgatgagcg tcagggcaaa acaccaaaag cctctccgat aacaccagaa
      241 caaatcgaaa tacgtaagct gaggaaaaag ctacaacgca ttgaaatgga gaatgaaata
      301 ttaaaaaagg ctaccgcgct cttgatgtca gactccctga acagttctcg ataatcggga
      361 aactcagagc gcattatcct gtggtcacac tctgccaagt gttcggggtt catcgcagca
      421 gctacagata ctggaaaaac cgtcctgaaa aaccagacgg cagacgggct gtattacgca
      481 gtcaggtact tgagctacat ggcatcagcc atggttcggc cggagcaaga agcatcgcca
      541 caatggcaac ccggagaggc taccagatgg gacgctggct tgctggcagg ctcatgaaag
      601 agctggggct ggtcagctgt cagcagccga ctcaccggta taaacgtggt ggtcatgaac
      661 atgttgctat ccctaactac cttgaaaggc agttcgccgt gaccgagcca aatcaggtgt
      721 ggtgcggtga tgtgacctgt atctggacgg gtaagcgctg ggcgtacctc gccg
//
LOCUS       DAJTMC010000379          762 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.392, whole genome shotgun sequence.
ACCESSION   DAJTMC010000379 DAJTMC010000000
VERSION     DAJTMC010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..762
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.392"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            165..594
                     /locus_tag="OO130_004349"
                     /pseudo
     CDS             165..594
                     /locus_tag="OO130_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          188 a          154 c          244 g          176 t
ORIGIN      
        1 gcccgctgca cggagcggga atttgcgaca attcctgccg gtatcaacgc cgacggcatg
       61 tattgccctg aatgcggtac ggttcactgg ccggatggcg ttattccgcc tttctgatgc
      121 ttccccgttt tgccgatatt tttcagcagg gaaaccgatg gcttaactgg ctggagaagc
      181 agccggtgca gatgagccgc ctggaacatt atgccggtca ggatgaaatt ggcctgcgtt
      241 acaacagcca ccggacaaaa cgagaagaaa accttgtgat gagtggcgac gagtttatgg
      301 aaaggttctc ctggcatgtg gcggataagg ggttccggat ggtgatacgg ggtcctgagt
      361 ccggtgaagc ggcgattact ggaagatgtg gtgtacgcca taacggagac agtgagaaaa
      421 acggcgaggc aaatcacaag gagagggatg tatcagcagt gactgaaggt tgacccgctg
      481 aagtgcgagc tgtgcggagg tcagatgcgg tttacggggc tgaagagggg ttaccgtctg
      541 gcagagcagg ttatgatgca tgagccgctg gctcgaatac ggtggtgcgg ctgagagtcg
      601 cagaggggaa gttgcgtcca tgttgagggg aacggagcaa aaaaccatca gagatacatt
      661 gaatcaatca atgtaagcaa tattaagtgt ctataccgat tacttgatga tcaggttgtg
      721 ttgaataaac gcgcattctt cggtgctttt ttaatctttt gg
//
LOCUS       DAJTMC010000380          733 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.393, whole genome shotgun sequence.
ACCESSION   DAJTMC010000380 DAJTMC010000000
VERSION     DAJTMC010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..733
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.393"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            <1..>733
                     /locus_tag="OO130_004350"
     CDS             <1..>733
                     /locus_tag="OO130_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000860751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MolR family transcriptional regulator"
                     /protein_id="HCR5367474.1"
                     /translation="RGDYSALISEFKTFWHSYQREWQLYMLAALPIEKTAQAWNVLSK
                     EPHVGVEFVMTHLQLAGLQGFIHSFSRYPQEALPVAQYFAAIELAPLIARAFNKLKTL
                     RENARTWLLKYPEHAITGLLASALDKAGEAQDNARAALRMLTENGHQPLLQEIARRYN
                     QPEVTDAVNALLALDPLDNHPTKIPTLPAFYQPSLWTRPVLKANAQSLPDSALLHLGE
                     MLRFPQEEALYPGLLQVKDACTADSL"
BASE COUNT          182 a          222 c          166 g          163 t
ORIGIN      
        1 agaggggaga ttattctgcg ctgatcagcg aatttaaaac cttctggcac tcctatcaac
       61 gggaatggca gctttacatg ttagccgccc tgcctatcga aaaaacagcg caggcgtgga
      121 atgtcctcag taaagagcca cacgtcggtg tcgagtttgt catgacgcac ctgcaactcg
      181 ctgggttaca gggttttatt cactcctttt cacgctatcc acaagaagct ctacctgtag
      241 cacaatattt tgcggctatc gaattagcgc cactgattgc ccgtgccttc aacaaactca
      301 aaacgctacg cgaaaacgcc cgcacctggc tgttgaaata cccggaacat gccattaccg
      361 gcctgctggc ttcggcgctc gacaaagccg gtgaagcaca ggataacgcc cgcgctgcct
      421 tgcgtatgct taccgaaaat ggtcatcagc cattactgca agaaatcgcc cggcgttata
      481 accagccgga agtaaccgac gcggtgaacg ctctgcttgc gctcgatccc ttagataatc
      541 acccaacaaa aatccccact cttccggcct tttatcagcc atcgctctgg acgcgcccgg
      601 tattaaaagc aaacgcccaa tcactgccag atagtgccct cctccacctc ggcgaaatgc
      661 tccgcttccc tcaggaagag gctctgtatc cgggattatt gcaggtgaaa gatgcctgta
      721 ccgccgactc act
//
LOCUS       DAJTMC010000381          720 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.394, whole genome shotgun sequence.
ACCESSION   DAJTMC010000381 DAJTMC010000000
VERSION     DAJTMC010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..720
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.394"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            <1..>720
                     /locus_tag="OO130_004351"
     rRNA            <1..>720
                     /locus_tag="OO130_004351"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          176 a          164 c          231 g          149 t
ORIGIN      
        1 aggaagggag taaagttaat acctttgctc attgacgtta cccgcagaag aagcaccggc
       61 taactccgtg ccagcagccg cggtaatacg gagggtgcaa gcgttaatcg gaattactgg
      121 gcgtaaagcg cacgcaggcg gtttgttaag tcagatgtga aatccccggg ctcaacctgg
      181 gaactgcatc tgatactggc aagcttgagt ctcgtagagg ggggtagaat tccaggtgta
      241 gcggtgaaat gcgtagagat ctggaggaat accggtggcg aaggcggccc cctggacgaa
      301 gactgacgct caggtgcgaa agcgtgggga gcaaacagga ttagataccc tggtagtcca
      361 cgccgtaaac gatgtcgact tggaggttgt gcccttgagg cgtggcttcc ggagctaacg
      421 cgttaagtcg accgcctggg gagtacggcc gcaaggttaa aactcaaatg aattgacggg
      481 ggcccgcaca agcggtggag catgtggttt aattcgatgc aacgcgaaga accttacctg
      541 gtcttgacat ccacggaagt tttcagagat gagaatgtgc cttcgggaac cgtgagacag
      601 gtgctgcatg gctgtcgtca gctcgtgttg tgaaatgttg ggttaagtcc cgcaacgagc
      661 gcaaccctta tcctttgttg ccagcggtcc ggccgggaac tcaaaggaga ctgccagtga
//
LOCUS       DAJTMC010000382          672 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.395, whole genome shotgun sequence.
ACCESSION   DAJTMC010000382 DAJTMC010000000
VERSION     DAJTMC010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 672)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 672)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..672
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.395"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            193..462
                     /locus_tag="OO130_004352"
     CDS             193..462
                     /locus_tag="OO130_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5367475.1"
                     /translation="MAQVNMSVRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQ
                     HNSWFRDQVAAGRQQLECGDVLPHDMVESSAAAWRDEMSRKIADK"
     gene            459..672
                     /locus_tag="OO130_004353"
                     /pseudo
     CDS             459..672
                     /locus_tag="OO130_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585073.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
BASE COUNT          167 a          154 c          197 g          154 t
ORIGIN      
        1 ctgtcagcgc cagtgatagg acaggtgaaa cctgtcaata cgtaccggct tacgccgggc
       61 ttcggcggtt ttactcctct gccatatgca acaacggagt gccgccttac atgccgctgg
      121 cgcggcatat cctggtgacg atatctgaac agttatatac tgtgtatata cgtggtaatg
      181 acgaggtggc aaatggcaca ggttaatatg agtgtaagaa tcgacgcaga actgaaggat
      241 gcttttatgg ccgctgcaaa aagcatggac cgtaatggct ctcagttaat ccgggatttt
      301 atgcgccaga ccgttgaacg gcagcacaat tcttggttcc gtgaccaggt tgcggcaggc
      361 cgtcagcaac tcgaatgcgg tgatgtgctt ccccatgaca tggtcgaatc ctctgctgct
      421 gcatggcggg atgaaatgag caggaagatt gctgataaat gatggagatg ttctggacca
      481 tgctggccat ccaggacagg aagcgcattc gtagtacatt gcggagcaga acctgatggc
      541 cgccattgaa ccggacgaac ggattggtta ttcagccagc agcctggcag ggcagccgta
      601 taaaggccgc aacggtcgtg tggaaggaac atctggacca cacaaggtag cgtgtaacgt
      661 tattttgtgt ga
//
LOCUS       DAJTMC010000383          656 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.396, whole genome shotgun sequence.
ACCESSION   DAJTMC010000383 DAJTMC010000000
VERSION     DAJTMC010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..656
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.396"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            215..574
                     /locus_tag="OO130_004354"
     CDS             215..574
                     /locus_tag="OO130_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HCR5367476.1"
                     /translation="MQIKVIYSLIDNMVNFKDKNMPAVIDKALDFIGAMDVSAPTPSS
                     MNESTAKGIFKYLKELGVPASAADITARADQEGWNPGFTEKMVGWAKKMEFGERTVIK
                     NPEYFSTYMQEELKALV"
BASE COUNT          211 a          127 c          131 g          187 t
ORIGIN      
        1 aactatataa ataaacataa ccatgccgtt gtgttcgccc ttcgattagc acctctggtt
       61 ttctttctta gctgaacaat ctggttatag cgtgcggact gaccgttaat tttctccatc
      121 ttactggata acacaccatg agctgctacc tttaaaacac ctgaatgtca acaggttaac
      181 tcgtgccata tcgttataat ccctttgata ttagatgcaa attaaggtca tatatagcct
      241 tatcgataac atggttaatt ttaaggataa gaatatgcct gctgtaatag ataaagccct
      301 ggatttcatt ggtgccatgg atgtatcagc gccaacacca agttcgatga atgaaagcac
      361 agcgaaggga atctttaaat atttaaaaga actgggagta cccgcgagtg ccgctgatat
      421 tacggcgcga gctgaccagg aaggctggaa ccctgggttc acggaaaaaa tggttggatg
      481 ggcaaaaaaa atggagtttg gtgaacgcac tgtaattaaa aatcctgaat acttttcaac
      541 atatatgcag gaagaactaa aagcactggt ctgagtttaa tttatatcag cataaatggg
      601 tcaggacgct tttaatcaca tataaaaagc gtcctttttc tcacaatcaa ataatc
//
LOCUS       DAJTMC010000384          655 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.397, whole genome shotgun sequence.
ACCESSION   DAJTMC010000384 DAJTMC010000000
VERSION     DAJTMC010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..655
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.397"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            17..>655
                     /locus_tag="OO130_004355"
     CDS             17..>655
                     /locus_tag="OO130_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 family protein"
                     /protein_id="HCR5367477.1"
                     /translation="MNIYIGWLFKLIPLIMGLICIALGGFVLESSGQSEYFVAGHVLI
                     SLAAICLALFTTAFIIISQLTRGVNTFYNILFPIIGYAGSIITMIWGWALLAGNDVMA
                     DEFVAGYVIFGIGMIAACVSTVAASSGHFLLIPKNAAGSKSDGTPVQAYSSLIGNCLI
                     AVPVLLTLLGFIWSITLLRSADITPHYVAGHVLLGLTAICACLIGLVATIVHQ"
BASE COUNT          137 a          146 c          161 g          211 t
ORIGIN      
        1 acctgaggag tttaaagtga acatatatat cgggtggctt ttcaaattaa tccctttgat
       61 tatgggctta atttgtatcg cgttaggtgg ctttgtgctg gaaagttcag ggcaaagcga
      121 gtatttcgtc gcgggtcatg tgctgatttc tctggcagcc atatgcctgg cattattcac
      181 taccgcattt attatcattt cgcagctcac gcgcggcgtt aatacgtttt acaatatatt
      241 gttccccatt attggctatg cagggtcaat tatcaccatg atatggggtt gggcactgtt
      301 agcaggcaat gatgtgatgg cagacgagtt tgtcgccggc tatgttattt tcggcattgg
      361 tatgattgcc gcatgtgtat cgacggtggc agcgtcgtcc ggtcactttc tgctcattcc
      421 caaaaatgca gcggggagca agagcgacgg aacaccggta caggcttatt cttcattaat
      481 cggtaactgc ctcattgccg ttcccgtttt actcaccctg ctcggtttca tttggtctat
      541 tacgctgtta cgtagtgctg acataactcc gcattatgtc gcgggtcacg tattgcttgg
      601 gttaaccgca atctgtgcct gtctaattgg ccttgttgcc acaattgtcc atcaa
//
LOCUS       DAJTMC010000385          623 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.398, whole genome shotgun sequence.
ACCESSION   DAJTMC010000385 DAJTMC010000000
VERSION     DAJTMC010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..623
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.398"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            <1..238
                     /gene="rclC"
                     /locus_tag="OO130_004356"
     CDS             <1..238
                     /gene="rclC"
                     /locus_tag="OO130_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HCR5367478.1"
                     /translation="MAFTTPLVTLSFLITTPEAWVSALGDAHYGFPYLSGAGRLVLKD
                     TLMLAGAVMIMADSAREILKQRSNESSSTLKTEY"
     gene            complement(398..>623)
                     /locus_tag="OO130_004357"
     CDS             complement(398..>623)
                     /locus_tag="OO130_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCR5367479.1"
                     /translation="SQVILRWNIQRGVVVIPKSTHQQRIEENFAIWDFSLTEKEMAQI
                     SSLDLGYVGESVKHFNPEFVRGCLAVKIHD"
BASE COUNT          180 a          139 c          122 g          182 t
ORIGIN      
        1 gatggcattt accacgccgc tggtaacact ctcattttta atcaccaccc cggaggcatg
       61 ggtatccgca ttgggtgacg ctcattatgg tttcccttat ttatccggtg ctggtcgctt
      121 ggtattgaaa gatactctga tgctggcagg tgctgtcatg ataatggcag attcggcgcg
      181 ggaaattctt aaacaacgca gtaatgaatc cagttcaacg ttaaaaactg aatattgata
      241 atccgaacac tgtctgttgt tcactttttc aggcgtcagg ccccctttct gatgatgaag
      301 actgacgcca tattaccgga gatatgacag agtgaagatc aatatcactc ccctgttaaa
      361 agattcttgt cgtctcaggt aaaatatatg attaatatca atcatgtatt tttacagcaa
      421 gacaaccacg aacaaattca ggattaaaat gttttaccga ctccccaaca taacccaaat
      481 caagcgaact aatttgtgcc atttctttct ctgtcagtga gaaatcccag atagcaaaat
      541 tttcttcgat acgctgttga tgtgtcgatt tcggaataac gaccactccc cgttgaatat
      601 tccaacgcag aatgacttga gaa
//
LOCUS       DAJTMC010000386          606 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.399, whole genome shotgun sequence.
ACCESSION   DAJTMC010000386 DAJTMC010000000
VERSION     DAJTMC010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..606
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.399"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            <1..>606
                     /locus_tag="OO130_004358"
     CDS             <1..>606
                     /locus_tag="OO130_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS91-like element ISSbo1 family transposase"
                     /protein_id="HCR5367480.1"
                     /translation="LACGTRILGVKEYICDKPECPHVRYVTNSCGSRACPSCGKKATD
                     LWIATQLNRLPDCDWVHLVFTLPDTLWPVFESNRWLLNDVCRLAVENLLYAARKRGQE
                     PGIFCAIHTYGRRLNWHPHVHVSVTCGGLNKHGQWKKLSFLKDAMRSRWMWNMRQLLL
                     KAWSEGLAMPESLSHITTESQWRSLVLKAGGKYWHVYMSKK"
BASE COUNT          140 a          154 c          188 g          124 t
ORIGIN      
        1 tgctggcctg cggcacgcgg atactgggag tgaaggaata catctgcgat aaacctgagt
       61 gcccccacgt cagatacgtc actaactcat gcggcagccg tgcctgcccg tcctgcggaa
      121 aaaaggccac agacctgtgg atagcgacac agctgaatcg tcttcctgac tgcgactggg
      181 tacacctggt cttcaccctg ccggacacgc tgtggccggt gttcgaaagc aaccggtggc
      241 tgctgaatga cgtgtgccgt ctggcggtgg agaatctgct gtatgccgcc cggaaacggg
      301 ggcaggaacc cggtatcttc tgcgccatcc acacgtatgg ccgtcgtctc aactggcatc
      361 cgcatgtaca tgtgtctgta acctgtggag gtctgaataa gcatggtcag tggaaaaagc
      421 tgagcttcct gaaagacgcg atgcgttcac ggtggatgtg gaatatgcgg cagctgcttc
      481 tgaaagcgtg gtcagagggg ctggcgatgc cggagtcgtt gtcacatatc acgacggaat
      541 cacagtggag aagcctggtg ctgaaagccg gaggaaaata ctggcatgtg tacatgtcga
      601 aaaaaa
//
LOCUS       DAJTMC010000387          597 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.400, whole genome shotgun sequence.
ACCESSION   DAJTMC010000387 DAJTMC010000000
VERSION     DAJTMC010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..597
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.400"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
BASE COUNT          222 a           87 c          101 g          187 t
ORIGIN      
        1 gtgtagtaat aaatcagccc taaatgggta aaatataaaa ctaatggatt acatctgatt
       61 tcaatctagc cattacaaaa cttaaatcaa gtgttaaata tgtaactaaa tgtaacttgt
      121 tatattaaaa tattaacctt aaggttttat taagtttaga aatgatagaa aagttgtaca
      181 tttggttttt attgcacaat tttaaaaaat catacaaatg gtgataactt actaataata
      241 catataaaaa atatttcggt gtagtccttt cgtcatgtaa aacgttcttg ttttttctcc
      301 gcacctccgt ggacaaattt ttactccaaa aagacgaggt ttgtcacggc ttgtgcgcaa
      361 gacatatcgc agcaatcagc gacgggcaag aaaaatgact gtctggtgat ttttgatagc
      421 ggaaaacgga gatttaaaag aaaacaaaat attttttgcg tagataacag cgtatttacg
      481 tgggttttaa tactttggta tgaacaaaaa aagaaaaata caacgcgcgg gtgagttatt
      541 aaaaatattt ccgcagacat actttccatc ataacgcagc gttaacaaaa tacaggt
//
LOCUS       DAJTMC010000388          592 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.401, whole genome shotgun sequence.
ACCESSION   DAJTMC010000388 DAJTMC010000000
VERSION     DAJTMC010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..592
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.401"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            312..592
                     /locus_tag="OO130_004359"
                     /pseudo
     CDS             312..592
                     /locus_tag="OO130_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          156 a          148 c          150 g          138 t
ORIGIN      
        1 acgggaatat ctgcggcagc gggcttgtaa tgggttaagt gataacagat gtctggaaat
       61 ataggggcaa atccacatcc ccgccagccc ggctaagatg gaggcacggc acagcccgca
      121 cacgacgctg aggctacaga gctgaaaacc cacaaggcca tgccccgcat ggccgggtaa
      181 agcggagccg gcggcgactt cctttgtgct ctgtccggga ccggcaggaa aaaggcgggg
      241 ataatgagat ggtcaccccc accctaacag cagtacgctg ttaaggtgaa tcccttaacg
      301 gagagaccat catacagaac atcacactta ttggtatcga tctcggaaag cattccttcc
      361 acattcattg tcaagacaag tctggtaagg ctcttctgcg taaaaaattt acccagtact
      421 aaactgatgc agtttctggc aacatgtccg ccctcagttg ttgtaatgga agcctgtgcg
      481 ggtgcacact ttttggctcg ccgtatctct gaattttgga catgaaacta agctgatttc
      541 tccacagttc gttcgcccgt ttgtcaaaag taataagaat gattttgttg at
//
LOCUS       DAJTMC010000389          592 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.402, whole genome shotgun sequence.
ACCESSION   DAJTMC010000389 DAJTMC010000000
VERSION     DAJTMC010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..592
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.402"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
BASE COUNT          190 a           93 c          106 g          203 t
ORIGIN      
        1 tgatatttgt atggtgcaat catgatgagc ttctcagaga ataattctct attaccgggc
       61 aaaatcagga acacaggaat taatatatat ttaacatccc ccttacaaag gtgagtttat
      121 atcgagttat tccctaagtt gtcgttactt attccaattc gttactataa atatggcaaa
      181 aatattacaa caaacagcgg ttaaaaaatt tggtgaagat tttttgtaat aatcctaatt
      241 agttatggga atttcgcttg atgtatctgc ataacaaaaa caatatgcgc catttttgca
      301 ttactaataa gaaaaagcaa aaaacatcga tattcatttt tggtcaatgg gttttctggc
      361 ctatcgttgt tattgtgcta ctggttatca ttttcatctt gccagcatat tggagcgtga
      421 tcaattttga tcagctgtga aggtggctga tgtgtagtga tacaaaaact tacgttatat
      481 cactgctaat gagatatccg tcatctgaac acttatgtct ttaattattc tcgtggttca
      541 ctataggcaa taagcacaaa agtgtaggat gttacaagaa tgattaggac tc
//
LOCUS       DAJTMC010000390          587 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.403, whole genome shotgun sequence.
ACCESSION   DAJTMC010000390 DAJTMC010000000
VERSION     DAJTMC010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..587
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.403"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(1..362)
                     /locus_tag="OO130_004360"
                     /pseudo
     CDS             complement(1..362)
                     /locus_tag="OO130_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233185.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
     gene            411..>587
                     /locus_tag="OO130_004361"
     CDS             411..>587
                     /locus_tag="OO130_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5367481.1"
                     /translation="MTKNTRFSPEVHQRAVRMVLESQGEYDSQWATICSIAPKIGCTP
                     ETLRVWVRQHERDTG"
BASE COUNT          154 a          158 c          132 g          143 t
ORIGIN      
        1 atatcaaaca gccctacacc aagtgcccag ttttcaatcc ccacacccgc ggcgacatcc
       61 gtggttaagg ttttccagtc cagcatctgg atctcatatt gcgggagttt atgtttgccg
      121 acccagaagt ctgcctctgg tgcgaagggt aaaaaacctc gcgttgtcag gtaaatatcg
      181 ctgaactgca tgatattttc attggcactg tcaccaaacc aggcatcgtt atactgctca
      241 cccacgttgc catcataggt aacgaccgca tttgccgttt taccgtcctg attataaaca
      301 cgctgattta aggtcaggtc aaaccaaccg ctcatctcat taccaaaacg tcccagtgaa
      361 ccgccccggg tttcctggag actaaacttc ctgagaaaga ggtaaacagg atgactaaaa
      421 atactcgttt ttcccccgaa gtccatcaac gggcagtccg tatggttctg gaaagtcagg
      481 gcgaatatga ctcacaatgg gcgacaattt gttccattgc accaaagatt ggctgtacac
      541 cggagactct gcgtgtctgg gtacgccagc atgagcggga taccgga
//
LOCUS       DAJTMC010000391          581 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.406, whole genome shotgun sequence.
ACCESSION   DAJTMC010000391 DAJTMC010000000
VERSION     DAJTMC010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..581
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.406"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            <1..289
                     /locus_tag="OO130_004362"
     CDS             <1..289
                     /locus_tag="OO130_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416251.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates concomitant with their translocation across the
                     cell membrane; involved in N,N'-diacetylchitobiose
                     transport; protein IIA transfers a phosphoryl group to IIB
                     which then transfers the phosphoryl group to the sugar;
                     IIC forms the translocation channel for the sugar uptake;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HCR5367482.1"
                     /translation="FIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTMPTGLGAFFN
                     TNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEESEEDIANALKF"
     gene            340..>581
                     /gene="chbA"
                     /locus_tag="OO130_004363"
     CDS             340..>581
                     /gene="chbA"
                     /locus_tag="OO130_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HCR5367483.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGK"
BASE COUNT          159 a          133 c          160 g          129 t
ORIGIN      
        1 gtttatcccg tttgtactgg tacaaccgat tctggcggca atcaccctcg cagcgtacta
       61 catgggcatt attcctccgg tgaccaatat tgcaccgtgg accatgccaa ccggtctggg
      121 agccttcttt aacaccaacg gtagcgtcgc tgcattgctg gtcgcactct tcaaccttgg
      181 tatcgcaacg ttaatttatc tgccctttgt tgtggtggct aacaaagcgc aaaacgcgat
      241 tgataaagaa gagagcgaag aagatatcgc taacgccctg aaattttaat cgatgccggt
      301 acggttaatc gtgccggtaa gaagagagag gaacgatgta tgatggatct cgataacatt
      361 cccgatacgc aaacagaagc tgaagagctg gaagaagtgg tgatggggct tatcatcaac
      421 tccggacaag cgcgcagcct ggcgtatgcg gcactgaaac aggcgaagca gggcgatttt
      481 gccgcagcaa aagccatgat ggatcagtca cggatggcat tgaatgaagc gcatctggta
      541 cagacgaaac tgattgaagg cgatgcgggc gaaggtaaga t
//
LOCUS       DAJTMC010000392          578 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.407, whole genome shotgun sequence.
ACCESSION   DAJTMC010000392 DAJTMC010000000
VERSION     DAJTMC010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..578
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.407"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            <1..>578
                     /locus_tag="OO130_004364"
     CDS             <1..>578
                     /locus_tag="OO130_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MolR family transcriptional regulator"
                     /protein_id="HCR5367484.1"
                     /translation="LRHINHPFALTLLIRVAGQTKRCHDRMTKAIAAFPHAAMAALTE
                     LLGQKEENSWRIMLMTMLISQPALAEQVLPWLSTPAVAVLKSYQQQLTQPSNHACADL
                     LPAVLVSPPWLSKKKKSVMPVLDLTPLPLESCCTLTETAEKEIHTRHRWHAHQIDIGQ
                     KEDIQNYLARLGFNRWNNGQYMKASDAVVELW"
BASE COUNT          165 a          149 c          132 g          132 t
ORIGIN      
        1 tgttgcgtca tatcaatcat ccgttcgcat tgacactgct tatacgtgta gccgggcaaa
       61 ctaaacgctg tcacgatcgg atgacgaaag ccattgctgc gttcccacat gcagcaatgg
      121 cggcactgac ggaacttctt gggcaaaaag aagagaacag ttggcgcatt atgctaatga
      181 caatgcttat ctcacaacca gcactggcag aacaggtcct tccctggctc tcgacacccg
      241 cagttgccgt actgaaatca taccagcaac aactgacaca gccctcaaac catgcctgcg
      301 ccgatctact gcctgccgta ttagtctcac cgccctggct ttcgaaaaag aaaaaatctg
      361 taatgccggt attggattta acgccattac cgttggagtc atgctgtaca ttgactgaga
      421 cggcagaaaa agagattcat acaaggcatc gctggcacgc acatcaaata gacatcggcc
      481 aaaaagagga tattcagaac tacctggcac gtctgggttt caatcgctgg aataatggcc
      541 agtatatgaa agcgtctgat gctgttgtag aactctgg
//
LOCUS       DAJTMC010000393          567 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.408, whole genome shotgun sequence.
ACCESSION   DAJTMC010000393 DAJTMC010000000
VERSION     DAJTMC010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..567
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.408"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(151..>567)
                     /locus_tag="OO130_004365"
     CDS             complement(151..>567)
                     /locus_tag="OO130_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR5367485.1"
                     /translation="FASDTIKIAYTGDGRVVWVGKDVTGIEPRNASVIEVPDITANRR
                     ITAPGYWFYRNDEFVFDYRLKAEDERDALLAQVSARTGEWEEDLLLGLISDEDKEKLK
                     ACRIYAKSLQAMDFSTITDKATYNAINWPERPDAAA"
BASE COUNT          118 a          152 c          144 g          153 t
ORIGIN      
        1 acacccggca gaacggggaa gtcatattcc ccgttctgtg tggtgacatg gctgaaaaca
       61 cttttcagaa tccctgcacg gttgtgtatg gaggttagtg taatggttgc gccgtccagc
      121 ggttctcccg tgggcgtttt aatcacacct ttaagctgcg gcatcgggac gctccggcca
      181 gttgatggcg ttatatgtgg ctttatctgt gatggtgctg aaatccatcg cctgcagcga
      241 tttcgcgtaa atacgacagg ctttcagctt ttctttatct tcgtcgctga ttaaccccag
      301 cagcaggtct tcttcccatt cccctgtccg ggcactgacc tgagccagaa gggcatcacg
      361 ctcatcttcc gctttgagtc tgtagtcaaa gacaaattca tcattgcggt aaaaccagta
      421 acccggcgcg gtaatgcggc ggttggcggt aatatcagga acttcaataa cactggcgtt
      481 acggggttca atgcctgtca catccttacc gacccacacc acgcgcccgt ctccggtgta
      541 agctatttta attgtgtcgc tggcgaa
//
LOCUS       DAJTMC010000394          558 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.409, whole genome shotgun sequence.
ACCESSION   DAJTMC010000394 DAJTMC010000000
VERSION     DAJTMC010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..558
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.409"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            <1..>558
                     /gene="fimZ"
                     /locus_tag="OO130_004366"
     CDS             <1..>558
                     /gene="fimZ"
                     /locus_tag="OO130_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbria biosynthesis transcriptional regulator
                     FimZ"
                     /protein_id="HCR5367486.1"
                     /translation="VIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDYLRTRP
                     VDLIIMDIDLPGTDGFTFLKRIKQIQSTVKVLFLSSKSECFYAGRAIQAGANGFVSKC
                     NDQNDIFHAVQMILSGYTFFPSETLNYIKSNKCSTNSSTVTVLSNREVTILRYLVSGL
                     SNKEIADKLLLSNKTVSAHKSNIYG"
BASE COUNT          180 a          102 c          106 g          170 t
ORIGIN      
        1 ggtgatcatt atggatactc atcctatcat cagaatgtct attgaagttc tgttgcaaaa
       61 aaacagtgaa ttgcagattg tcctgaaaac ggatgattac cgcataacca tcgattatct
      121 ccgaacccgt cctgttgatt taatcattat ggatatagac ttgcccggaa cagacggttt
      181 taccttcctg aaaaggatca aacaaatcca gagcacagtg aaagtgttat ttttatcatc
      241 gaaatcagaa tgcttttatg ctggcagagc gatacaagct ggtgctaacg gttttgtcag
      301 taaatgcaat gatcagaatg atatttttca tgccgttcag atgatcctct ccggatacac
      361 gttttttccc agcgaaacgc ttaactatat aaaaagcaat aaatgtagta cgaatagttc
      421 aacggtcact gtgctatcta atcgtgaagt gaccatatta cgttatctgg ttagcggatt
      481 atctaataaa gaaattgccg ataagttatt gcttagcaat aaaacagtta gtgcgcataa
      541 atctaatatt tatggcaa
//
LOCUS       DAJTMC010000395          545 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.410, whole genome shotgun sequence.
ACCESSION   DAJTMC010000395 DAJTMC010000000
VERSION     DAJTMC010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..545
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.410"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(50..>545)
                     /locus_tag="OO130_004367"
     CDS             complement(50..>545)
                     /locus_tag="OO130_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="porin"
                     /protein_id="HCR5367487.1"
                     /translation="SVDYNIEGFGFVGAYSKSDRTNEQAGDGYGDNAEVWSLAAKYDA
                     NNIYAAMMYGETRNMTVLANDHFANKTQNFEAVVQYQFDFGLRPSLGYVYSKGKDLYA
                     RDGHKGVDADRVNYIEVGTWYYFNKNMNVYTAYKFNLLDKDDAAITDAATDDQFAVGI
                     IYQF"
BASE COUNT          150 a          120 c          111 g          164 t
ORIGIN      
        1 tcaggctagt gttatgtttt acagtaacac gccgcggcgc gttggctgat tagaactggt
       61 agatgatacc aactgcaaac tggtcatcag ttgcggcatc ggtaatcgct gcatcgtctt
      121 tatccagcag gttaaatttg tatgctgtgt agacgttcat gttcttattg aagtagtacc
      181 aggtaccaac ttcgatataa tttacgcggt cagcatcaac acctttatgt ccatcacgag
      241 cataaagatc tttgcctttg gaatatacgt agcctaaaga cggacgtaaa ccgaagtcga
      301 actgatactg tacaacagct tcaaagtttt gggttttatt tgcaaaatga tcattagcca
      361 aaacggtcat gttgcgggtt tcaccgtaca tcattgctgc atagatatta tttgcatcat
      421 acttggctgc taatgaccac acttcagcgt tatcaccgta gccgtcacca gcttgttcat
      481 tagtacgatc agatttgcta tatgcaccta caaaaccaaa accttcgatg ttgtagtcta
      541 cagac
//
LOCUS       DAJTMC010000396          539 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.411, whole genome shotgun sequence.
ACCESSION   DAJTMC010000396 DAJTMC010000000
VERSION     DAJTMC010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..539
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.411"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            <1..>539
                     /locus_tag="OO130_004368"
     CDS             <1..>539
                     /locus_tag="OO130_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000878026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS110-like element ISEc11 family transposase"
                     /protein_id="HCR5367488.1"
                     /translation="RRQDFLTFFGNREPCLIGMEACGGSQHWARELTKLGHKVRLLQA
                     RFVKAFVMGNKNDVMDARAIWMAVQQPGKEIAVKTEEQQSVLVLHRTRMQLVKFRTAQ
                     INALHGTLLEFGETIHKGRAAMEREFPEALERMKERLPPYLIMVLENQYNRLNELDSL
                     IEDIEKQLTSVARQNETCK"
BASE COUNT          146 a          123 c          159 g          111 t
ORIGIN      
        1 gcgtagacag gattttctga cgttcttcgg caaccgtgag ccatgcctga ttggtatgga
       61 ggcctgtgga ggttctcagc actgggcacg ggaactgaca aaacttggtc ataaagtccg
      121 gttgttgcag gcccgcttcg ttaaggcatt cgtcatgggc aataagaatg atgtgatgga
      181 tgcccgggct atctggatgg cggttcagca gccgggtaaa gaaatcgccg taaaaacaga
      241 agaacagcag tcggtactgg ttctgcaccg tacccgcatg caactggtga agttccggac
      301 cgcacaaatt aatgccctgc acgggacgtt actggagttt ggtgaaacca tccacaaagg
      361 ccgggcagcg atggagcggg agttccccga agcactggaa cggatgaaag agagactgcc
      421 accgtatctc attatggttc tggaaaacca gtacaaccga ctgaatgagc tggactcact
      481 gatagaggat attgaaaaac agcttaccag cgtggcgagg cagaatgaaa cctgtaagc
//
LOCUS       DAJTMC010000397          532 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.412, whole genome shotgun sequence.
ACCESSION   DAJTMC010000397 DAJTMC010000000
VERSION     DAJTMC010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..532
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.412"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            1..532
                     /locus_tag="OO130_004369"
                     /pseudo
     CDS             1..532
                     /locus_tag="OO130_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417134.2"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
BASE COUNT          170 a          108 c          143 g          111 t
ORIGIN      
        1 catatggttc ggcaaataag acgattatta aagataaagg aacaatgtca gttttaacga
       61 atgctaaagc tgatgcgacc cgaatagata acggcggggt aatggatgtt accggaaacg
      121 cgaccaatac cataatcaat ggtggtacac agaatattaa taatcatggt attgccacgg
      181 gtaccaatat caacagcgga acacaaaata tcaagagcgg cgggaaagct gacacgacaa
      241 atatttccac cgggagccgg caggttgttg agaaagatgg tacggcaact ggcagcaata
      301 ttagcgccgg aggctcgctg attgtctata ccggcggtat agcacatggg gttaaccagg
      361 agacgggcag tgctttagtt gccaacacgg gcgcagggac tgatatcgaa ggatacaaca
      421 agctctctca cttcactatt acccggaggg gaggctaatt atgttgtgct ggaaaatacc
      481 ggcgaactga cggtagtggc taaaacctcg gcgaaaaata ctaccgttga tg
//
LOCUS       DAJTMC010000398          527 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.413, whole genome shotgun sequence.
ACCESSION   DAJTMC010000398 DAJTMC010000000
VERSION     DAJTMC010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..527
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.413"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            <1..325
                     /gene="dsbG"
                     /locus_tag="OO130_004370"
     CDS             <1..325
                     /gene="dsbG"
                     /locus_tag="OO130_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HCR5367489.1"
                     /translation="SGKVQLRTLLVGVIKPESPATAAAILASKDPAKTWQQYKASGGK
                     LKLNVPANVSTEQMKVLSDNEKLMDDLGANVTPAIYYMSKENTLQQAVGLPDQKTLNI
                     IMGNK"
BASE COUNT          191 a           98 c          114 g          124 t
ORIGIN      
        1 ttctggcaaa gtgcaattaa gaacattgtt ggttggggtt atcaagccag aaagcccggc
       61 gacagcagcg gcaattcttg cctccaaaga tcccgcaaaa acctggcaac aatataaagc
      121 ctctggtggc aagcttaagc taaacgtgcc tgcaaacgta agtacagagc aaatgaaagt
      181 gttaagtgac aatgagaaac tgatggacga tctgggggca aatgtcacgc cggctatcta
      241 ttacatgagt aaggaaaata cgctacaaca ggccgtgggg ttgcccgatc agaaaacgct
      301 taatatcatt atggggaata aataaaaggt aattcatgga tggttgaact atatctctgc
      361 ttttcaacca tcctaaaaag catgaattgc tcgctctgcc ctaagtggct gtgctatttt
      421 agaaaaactt aatcctgagt taagcaaaaa aacataatca ataaaaatat atacgcagaa
      481 aaacattgat taagtgaata tatcatggaa gaaaaatata accggag
//
LOCUS       DAJTMC010000399          518 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.414, whole genome shotgun sequence.
ACCESSION   DAJTMC010000399 DAJTMC010000000
VERSION     DAJTMC010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..518
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.414"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(<1..350)
                     /locus_tag="OO130_004371"
     CDS             complement(<1..350)
                     /locus_tag="OO130_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5367490.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYG"
     gene            complement(355..>518)
                     /gene="hyfE"
                     /locus_tag="OO130_004372"
     CDS             complement(355..>518)
                     /gene="hyfE"
                     /locus_tag="OO130_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="interacts with formate dehydrogenase to produce an
                     active formate hydrogenlyase complex which cleaves formate
                     to dihydrogen and carbon dioxide; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HCR5367491.1"
                     /translation="LVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLD
                     VNNLTALKG"
BASE COUNT          150 a          147 c          127 g           94 t
ORIGIN      
        1 ccgtagtaat cgcacagtgt aacgggtgaa agctcgccgt gtgccacttc atgacgcatg
       61 tagccaatcg agtaaacacc ggtgagaaag ccaatcacac caagaatggc gaggaacaaa
      121 ccgcccagac catcaatatg aagccacagt cccgcagcga atatttctcc tgcatcagcg
      181 gcagtttgga ccacccagag tgccagaatc agcagcagtg tgatccctaa gctatgtaat
      241 acagtcaccg tacaagtcgc agagagcccc cgtttccggc aggcaaaaca gagcagcgaa
      301 aaaagcagcg gcgtgagcag gagtaaagcg aacatcacag aataactcat ctcattatcc
      361 cttcagcgcg gtcaagttgt tcacgtccag cgtgccgtgg gtacgccata tttttcttgc
      421 cagtaacacc atcacaatga cggcgaagat ggcgtcggta gcgataccta tttccaccag
      481 ttccggtgct cgccaggcaa gaagcgccag caccagat
//
LOCUS       DAJTMC010000400          501 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.415, whole genome shotgun sequence.
ACCESSION   DAJTMC010000400 DAJTMC010000000
VERSION     DAJTMC010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..501
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.415"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(<1..>501)
                     /locus_tag="OO130_004373"
     CDS             complement(<1..>501)
                     /locus_tag="OO130_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HCR5367492.1"
                     /translation="GAGRHFQLVLTTQAQRAEEARQQAISGGTEPSAFPDTLPGYTEY
                     GRDNGIRLSAVWLTHDPEYPENLPAAPLVRYGWTPRGELAAVYDRSNTQVRSFTYDDK
                     YRGRMVAHRHTGRPEIRYRYDSDGRVTEQLNPAGLSYTYQYEKDRITITDSLNRREVL
                     HTQGEAG"
BASE COUNT           81 a          159 c          153 g          108 t
ORIGIN      
        1 ccggcttcac cctgcgtgtg cagcacttca cggcggttca ggctgtcggt gatggtgatg
       61 cggtccttct catactgata cgtgtagctt aagcctgccg ggttaagctg ttctgtcacc
      121 cgcccgtcgc tgtcgtaacg gtaacggatt tccggccggc ccgtgtgacg gtgcgccacc
      181 atccggcccc ggtatttatc atcgtaagta aagctacgca cctgtgtatt gctgcggtca
      241 tacactgccg ccagttcgcc gcgcggcgtc cagccatagc gcaccagcgg cgcggcaggt
      301 aaattctccg ggtattccgg gtcgtgcgtc agccacacgg cagacagacg gatgccgttg
      361 tcccggccat attcggtgta acccggcagg gtatcaggaa aagcggacgg ttccgtcccg
      421 ccggaaatgg cctgctgccg ggcttcttct gcccgctgcg cctgcgtggt cagtaccagt
      481 tggaagtgac gcccggcacc a
//
LOCUS       DAJTMC010000401          486 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.416, whole genome shotgun sequence.
ACCESSION   DAJTMC010000401 DAJTMC010000000
VERSION     DAJTMC010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..486
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.416"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            1..464
                     /locus_tag="OO130_004374"
                     /pseudo
     CDS             1..464
                     /locus_tag="OO130_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          109 a          126 c          126 g          125 t
ORIGIN      
        1 ccgttactgt tcattgtccg cgctgtcatt ccgatgaagt ttatcgacat ggccgtagtt
       61 gttcccggca tgagcgtttc cgttgtcgct catgtaaacg tgttttccag ctcacttact
      121 cttatgaagc cagaaaaccc ggcgttaagg agcagatcgt tgaaatggca cataacggcg
      181 cagggggccg tgataccgcc aggacactga aaatcggcat caataccgtc atacggacat
      241 taaaaagctc ccgccccggc ggataacatc tgcgccagtc gctcacgctg atgttgccct
      301 tatctgtgaa cttgatgagc agtggcgctt cgtcggcagc aaggctgcaa caacactggc
      361 tctggtatgc gtacaacact aaaactggcg gtgtgttggc ttacactttc gggccacgga
      421 atgatgagac ctatcgggaa ctgttagccc tgtttactcc gttctgtata tattaggccg
      481 ttggtg
//
LOCUS       DAJTMC010000402          484 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.417, whole genome shotgun sequence.
ACCESSION   DAJTMC010000402 DAJTMC010000000
VERSION     DAJTMC010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..484
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.417"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(<1..312)
                     /locus_tag="OO130_004375"
     CDS             complement(<1..312)
                     /locus_tag="OO130_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5367493.1"
                     /translation="MNSQTTKDIPCFRSYLPDALRLRFEDKLTIRAIAQRLGLSHSTI
                     HTLFQRFLASGIAWPLPDSVSFAQLDAILYANRKKELTEPQIREGSWRKERRTSYSRE
                     FK"
BASE COUNT          129 a           98 c          132 g          125 t
ORIGIN      
        1 cttaaattca cggctatagc tggttcgccg ttcttttcgc catgagcctt ctctgatttg
       61 aggctctgtt aattccttct ttctgttggc ataaaggatg gcgtcaagtt gagcgaatga
      121 aactgaatcg ggcaatggcc atgcgatacc ggatgcaaga aatcgctgaa aaagcgtatg
      181 tattgtggaa tgactgagac ccagacgctg agcgatggcc cggatggtca gtttatcttc
      241 aaatcttaaa cgcagggcat caggcaaata agaacggaag cagggaatat cttttgttgt
      301 ctgggaattc atcgttcgtg tccatcaata tagatgggcg cgattgttgc cagacaggac
      361 aattttcaca agacgtcgct gatggggcgc ttaccttacc gcaggacagg tggtaactga
      421 aaaatagttt gctgaggcga ctggaaacag aagggcaaga tcgattccga cagcagtgat
      481 ggtc
//
LOCUS       DAJTMC010000403          482 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.418, whole genome shotgun sequence.
ACCESSION   DAJTMC010000403 DAJTMC010000000
VERSION     DAJTMC010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..482
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.418"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(236..>482)
                     /gene="dgcZ"
                     /locus_tag="OO130_004376"
     CDS             complement(236..>482)
                     /gene="dgcZ"
                     /locus_tag="OO130_004376"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HCR5367494.1"
                     /translation="AANDEEACRAGVRICQLVDNHAITHSEGHINITVTAGVSRAFPE
                     EPLDVVIGRADRAMYEGKQTGRNRCMFIDEQNVINRV"
BASE COUNT          127 a          116 c          100 g          139 t
ORIGIN      
        1 acaaatggga tgactaatga acggagataa tccctcacct aaccggcccc ttgttacagt
       61 tgtgtacaag gggcctgatt tttatgacgg cgaaaaaaaa ccgccagtaa accggcggtg
      121 aatgcttgca tggatagatt tgtgttttgc ttttacgcta acaggcattt tcctgcactg
      181 ataacgaatc gttgacacag tagcatcagt tttctcaatg aatgttaaac ggagcttaaa
      241 ctcggttaat cacattttgt tcgtcaataa acatacagcg atttcttccc gtttgcttac
      301 cctcatacat tgcccggtct gctcttccaa tgaccacatc cagaggctct tcaggaaatg
      361 cgcgactgac acctgctgtc acggtaatgt tgatatgccc ttcagaatgt gtgatggcat
      421 ggttatcgac taactggcaa attctgacac ctgcacgaca tgcttcttca tcattagccg
      481 ct
//
LOCUS       DAJTMC010000404          482 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.419, whole genome shotgun sequence.
ACCESSION   DAJTMC010000404 DAJTMC010000000
VERSION     DAJTMC010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..482
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.419"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(<1..332)
                     /locus_tag="OO130_004377"
     CDS             complement(<1..332)
                     /locus_tag="OO130_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCR5367495.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLTALALSLFTGL
                     LLVLVAQPILFLLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPE"
BASE COUNT          148 a          104 c          114 g          116 t
ORIGIN      
        1 ctttccggta aaaataaaaa cggtaaatag agcgcaatat cggaaataac atcgccagtt
       61 tcattcaaaa tcgcccccag acgtgtttgc tgattgcact cacgcgccaa catgccatcc
      121 agcgcattga gcgccatacg gataaaaagc acgatgggca atagcaaaaa gaggatgggt
      181 tgtgccacca acaccaacag caatccggta aaaagagaaa gcgccagtgc cgtaagagtg
      241 atgtgattcg ctgtaacgtg gtgcttataa agccaaaaca tcgtcggcct taacagcgac
      301 tgaaagagcg gttttatctg gtagagcgtc atttatcatt ccgtgatatt cagtttgctc
      361 tatggtgcat caattgtgat ggcggagata tcacaacgcg gagcatcaac gtaaattcgg
      421 aatatatcaa gatgtggtgg atggttttcc tacacatctg tttgattacc gactggatgt
      481 ca
//
LOCUS       DAJTMC010000405          477 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.420, whole genome shotgun sequence.
ACCESSION   DAJTMC010000405 DAJTMC010000000
VERSION     DAJTMC010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..477
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.420"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(<1..>477)
                     /locus_tag="OO130_004378"
     CDS             complement(<1..>477)
                     /locus_tag="OO130_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HCR5367496.1"
                     /translation="LEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGG
                     SPHDCAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEA
                     RHIKMAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPIT"
BASE COUNT          115 a          113 c          129 g          120 t
ORIGIN      
        1 cagtgatcgg cgtggcggca atagaaacat atgcttcgat agcgtgcgtt aaggcatcca
       61 tacccgttgc ggcggtcagt gacttcggca taccaatcat cagagaggag tcattgacag
      121 aaagcagcgg agtgacatgt ttatcaacaa tcgccatttt gatatgacgc gcttcgtcag
      181 tgatgatgca gaaacgggtc atttcagagg ccgtacccgc agtggtgttg atggcgatca
      241 tcggcagctg cggttttgca gagcggtcaa cgccttcgta atcgcgaata tcgccgccat
      301 tggctgccac cagcgcaata ccttttgcgc agtcgtgtgg agaaccaccg cctaaggaga
      361 tcacgctatc gcaattattc tctttaagta atttcaaacc tgcggcgacg ttttccgtgg
      421 tggggttagg ttgggtgcca tcataaataa cgctaaaaat attgcgttct tccagtg
//
LOCUS       DAJTMC010000406          477 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.421, whole genome shotgun sequence.
ACCESSION   DAJTMC010000406 DAJTMC010000000
VERSION     DAJTMC010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..477
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.421"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            46..>477
                     /locus_tag="OO130_004379"
     CDS             46..>477
                     /locus_tag="OO130_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCR5367497.1"
                     /translation="MSVVISGALTDGAGIPMSGCQIILKSRVNTSEVVMRTRADVVTG
                     NNGEYSFEAQVGKYCVYLKRDWRDEYCVGDIAVYDDSKPGTLNDFLTAPDEGDLKPDV
                     VKRFEEMVAQAQQSAGAAAGNAQQTAQDVAAAAGYARAAEQA"
BASE COUNT          116 a          117 c          147 g           97 t
ORIGIN      
        1 ctctccggaa ggttcttcct gatttaattt atcgcgagaa aaactatgtc tgtagtgata
       61 tcaggtgcgc tgactgatgg cgcaggcatc cccatgtccg gatgccagat tattctgaaa
      121 tcccgtgtaa acacctcaga agtggtgatg cgtaccaggg ctgatgtagt gaccggaaat
      181 aacggtgaat attcgtttga ggcacaggtc ggaaaatatt gcgtgtatct gaaacgggac
      241 tggcgcgacg agtactgtgt tggcgacatt gctgtatacg acgactcaaa gcccggcacg
      301 ctgaatgact ttctgactgc ccccgatgaa ggcgacctga agccggatgt cgtcaaacgc
      361 tttgaggaaa tggtggcgca ggcgcagcag agcgccgggg ccgcagccgg aaacgcacag
      421 cagacggcgc aggatgtggc ggcagccgca ggttatgccc gcgcagcaga acaggcc
//
LOCUS       DAJTMC010000407          469 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.422, whole genome shotgun sequence.
ACCESSION   DAJTMC010000407 DAJTMC010000000
VERSION     DAJTMC010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..469
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.422"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            <1..>469
                     /locus_tag="OO130_004380"
     CDS             <1..>469
                     /locus_tag="OO130_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HCR5367498.1"
                     /translation="DIALPGPLPFILSRTYSSYRTKTPAPVGSLGPGWKMPADIRLQL
                     RDNTLILSDNGGRSLYFEHLFPGEDGYSRSESLWLVRGGVAKLDEGHRLAALWQALPE
                     ELRLSPHRYLATNSPQGPWWLLGWCERVPEADEVLPAPLPPYRVLTGLVDRFGR"
BASE COUNT           79 a          148 c          159 g           83 t
ORIGIN      
        1 ccgacatcgc cctgcccggc ccgctgccgt tcatcctctc ccgcacctac agcagttacc
       61 ggacaaaaac gcccgcgccg gtggggagcc tcggccccgg ctggaaaatg cctgcggata
      121 tccgcttaca gctgcgcgat aacacactga tactcagtga taacggcggc agaagcctgt
      181 attttgagca cctgtttccc ggtgaggacg gttacagccg cagcgagtca ctgtggctgg
      241 tgcgcggcgg cgtggcgaaa ctggatgaag gtcaccggct ggccgcactc tggcaggcgc
      301 tgccggaaga actccgctta agtccgcatc gttatctggc gacaaacagt ccgcaggggc
      361 cgtggtggct gctcggctgg tgtgagcggg tgccggaagc ggatgaggtg ctgcctgcgc
      421 cgctgccgcc gtaccgggta ctgaccgggc tggtggaccg cttcgggcg
//
LOCUS       DAJTMC010000408          466 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.423, whole genome shotgun sequence.
ACCESSION   DAJTMC010000408 DAJTMC010000000
VERSION     DAJTMC010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..466
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.423"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            107..466
                     /locus_tag="OO130_004381"
                     /pseudo
     CDS             107..466
                     /locus_tag="OO130_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          121 a          115 c          110 g          120 t
ORIGIN      
        1 tttaacgtaa ggtgagagga tttttaagtg tatccataga ttaaaaatga caatgcctcc
       61 aattagttga attaggggtt ataatacccg cttttaaggt tctttcatgg tcacagttaa
      121 ctttcattgc ccccgttgcc agtccgttca ggtttaccgt cacgggcaga acccaaaagg
      181 acatgatcga ttccgttgcc gtgactgtca ccgggtgttt cagctcactt actgttacga
      241 ggcccgtaaa ccgggagtta aaaaccaaat cactgaaatg gcttcaacgg tgccggagtt
      301 cgcgatactg cccgtacgct gaaaatcggc atcaataccg tcatacggac attaaaaagc
      361 tcccgccccg gcggataaca tctgcgccag tcgctcacgc tgatgttgcc cttatctgtg
      421 aacttgatgg gcagtggagc ttcgttggca gcaaggcgca acaaca
//
LOCUS       DAJTMC010000409          457 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.424, whole genome shotgun sequence.
ACCESSION   DAJTMC010000409 DAJTMC010000000
VERSION     DAJTMC010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..457
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.424"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            41..310
                     /locus_tag="OO130_004382"
                     /pseudo
     CDS             41..310
                     /locus_tag="OO130_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
BASE COUNT          142 a           90 c          119 g          106 t
ORIGIN      
        1 tgaattaaat caattatggc gatgaattac aaggggttaa atgctgccgc agcatagcga
       61 tattgaaata gcctggtatg cttcgataca gcaggagctg aatgtctgga agaccgtcac
      121 cacacagttc tacatccagg aattcagtga gcatattgcg ccacttcagg atgctgtaga
      181 tctggagatc gcaacggagg aagaaaactc gttgctggaa gcctagaaga agtatcgggt
      241 gttgctgaac cgtgttgata catcaactgc acctgatatt gagtggccgg aagaaccaga
      301 cacaatgtaa gcgaaaaaga aaaaccgcag acacgacgta tgcaggacgt gctgcggttg
      361 gctggtgaac tttcgatagt gcgagtattg aatgatttcc agccgttacc gattttatat
      421 aactgcagtg gaattattca acacacaggc aaaacgg
//
LOCUS       DAJTMC010000410          447 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.425, whole genome shotgun sequence.
ACCESSION   DAJTMC010000410 DAJTMC010000000
VERSION     DAJTMC010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..447
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.425"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            35..447
                     /locus_tag="OO130_004383"
                     /pseudo
     CDS             35..447
                     /locus_tag="OO130_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          101 a          115 c          121 g          110 t
ORIGIN      
        1 atttagtgta tgatggtgat tttaaggtgc ttgcgtggct tccatttcca tcagatgtcc
       61 ttcctgctcc gctactgaag gcgtggtgcg taacggcaaa agcactgccg gacatcagcg
      121 ctatctctgc tctcattgcc gtaaaacatg gcaactacag ttcacttaca ccgcctctca
      181 gcccggtacg caccagaaaa tcattgatat ggccatgaat ggcgtcggat gtcgcgccag
      241 tgcacgcatt atgggcgttg gcctcaacac ggttttacgt cacttaaaaa actcaggccg
      301 cagtcggtaa cctcgcgcat acaaccgggc agtgatgtga ttgtctgcgc tgaaatggac
      361 gaacagtggg gctacgtcgg tgctaaatca cgtcagcgct ggctgtttta cgcgtatgac
      421 aggatacgga ggacggttgt ggcgcac
//
LOCUS       DAJTMC010000411          443 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.426, whole genome shotgun sequence.
ACCESSION   DAJTMC010000411 DAJTMC010000000
VERSION     DAJTMC010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.426"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(357..>443)
                     /locus_tag="OO130_004384"
     CDS             complement(357..>443)
                     /locus_tag="OO130_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008786105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HCR5367499.1"
                     /translation="RQMQARRVLQQMSDERLRDIGLRREDVE"
BASE COUNT          119 a          102 c           83 g          139 t
ORIGIN      
        1 ttcattgtat cctctggcag gtgtataatt agcgaattga tactagcaac gaagcttcgg
       61 ttatctattt gcagagaatt gtttcattat gaactgtgag cgcgataact aatttttgat
      121 acaaaaattt cattaatgtg atctaaaacg aatcgcttta ttcaccgctg atgattttgt
      181 tctattcctt tttgtgacag atcccccgga cgcatagatg atgaccccca gagagacagc
      241 tcaggtcatt atctgtatgt aaatcagaaa aatatgttgt aaacgacttt tatggttgga
      301 ttttgtagca attttgaatg cagacaggca gcatttgcca acacgccgcc catagttcac
      361 tccacatcct ccctgcgtaa cccgatatcc ctcaaccgtt catcactcat ctgctgtaac
      421 acccgtcggg cctgcatttg ccg
//
LOCUS       DAJTMC010000412          438 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.427, whole genome shotgun sequence.
ACCESSION   DAJTMC010000412 DAJTMC010000000
VERSION     DAJTMC010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..438
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.427"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(<1..200)
                     /locus_tag="OO130_004385"
     CDS             complement(<1..200)
                     /locus_tag="OO130_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-mannonate oxidoreductase"
                     /protein_id="HCR5367500.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYKVNLIGGEQQIA"
     gene            complement(289..414)
                     /locus_tag="OO130_004386"
     CDS             complement(289..414)
                     /locus_tag="OO130_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001397278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCR5367501.1"
                     /translation="MIGLISALKMPEVKDRDLSAQEDAAEDQPRVVRAAQPSRSL"
BASE COUNT          126 a           99 c           88 g          125 t
ORIGIN      
        1 gcaatttgct gttcgccgcc gattaagttg accttataat atccccagtc actgaaatgt
       61 tctgtagcaa gaatatcggc atacacgccc tgatgcgcac ggtgaaatgc accaaagcct
      121 aaatgaacaa ttcttggggc caggttatta cgatcataaa cagggagtgt cgcttttgct
      181 gataacaaat tatttcccat aacaattcct taaatttaaa tatggcaagc tatatgtttt
      241 gttatatgaa taaaaatccc ctctccggta agagaaggga ttgagggttt acagacttct
      301 ggaaggttgc gcagctctta caacacgcgg ttgatcttcc gcagcgtctt cctgcgcact
      361 taaatcacgg tctttcacct ctggcatttt cagcgcagag attaaaccaa tcactgaata
      421 tgccatgatc ataatggc
//
LOCUS       DAJTMC010000413          422 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.428, whole genome shotgun sequence.
ACCESSION   DAJTMC010000413 DAJTMC010000000
VERSION     DAJTMC010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..422
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.428"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            <1..>422
                     /locus_tag="OO130_004387"
     CDS             <1..>422
                     /locus_tag="OO130_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HCR5367502.1"
                     /translation="GRVAVKESNQRWCSDGFEFCCDNGERLRVTFALDCCDREALHWA
                     VTTGGFNSETVQDVMLGAVERRFGNDLPSSPVEWLTDNGSCYRANETRQFARMLGLEP
                     KNTAVRSPESNGIAESFVKTIKRDYISIMPKPDGLTAA"
BASE COUNT          106 a           98 c          138 g           80 t
ORIGIN      
        1 ggcagagtgg ccgtgaaaga aagcaatcag cgatggtgct ctgacgggtt cgagttctgc
       61 tgtgataacg gagagagact gcgtgtcacg ttcgcgctgg actgctgtga tcgtgaggca
      121 ctgcactggg cggtgactac cggcggcttc aacagtgaaa cagtacagga cgtcatgctg
      181 ggagcggtgg aacgccgctt cggcaacgat cttccgtcgt ctccagtgga gtggctgacg
      241 gataatggtt catgctaccg ggctaatgaa acacgccagt tcgcccggat gttgggactt
      301 gaaccgaaga acacggcggt gcggagtccg gagagtaacg gaatagcaga gagcttcgtg
      361 aaaacgataa agcgtgacta catcagtatc atgcccaaac cagacgggtt aacggcagca
      421 aa
//
LOCUS       DAJTMC010000414          417 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.429, whole genome shotgun sequence.
ACCESSION   DAJTMC010000414 DAJTMC010000000
VERSION     DAJTMC010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..417
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.429"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            <1..>417
                     /locus_tag="OO130_004388"
     CDS             <1..>417
                     /locus_tag="OO130_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HCR5367503.1"
                     /translation="TDCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDSDDKFETHRQE
                     MAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGETCIDCHKGIAH
                     FPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSR"
BASE COUNT          140 a           85 c           88 g          104 t
ORIGIN      
        1 tcacagattg ccatattcca aagtcaggga tggattattt atttgctaaa ttaaaagcat
       61 ctaaagatat ttatcatgaa tttgttagcg gcaaaataga cagtgacgat aagttcgaaa
      121 ctcatcgcca ggaaatggcc gaaacagtat ggaaagaatt aaaagcaact gactctgcaa
      181 cgtgccgtag ttgccattct tttgatgcca tggatattgc ctcgcaaagt gaatctgcgc
      241 agaaaatgca taacaaagca caaaagggcg gcgaaacctg tatcgattgt cataaaggca
      301 ttgcccattt tccgccagaa ataaaaatgg atgacaacgc ggcgcatgag ctggaaagtc
      361 aggccgctac ttcagtgact aatggcgcac atatttatcc tttcaaaact tctcgca
//
LOCUS       DAJTMC010000415          407 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.430, whole genome shotgun sequence.
ACCESSION   DAJTMC010000415 DAJTMC010000000
VERSION     DAJTMC010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..407
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.430"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            <1..>407
                     /locus_tag="OO130_004389"
     CDS             <1..>407
                     /locus_tag="OO130_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR5367504.1"
                     /translation="EPSDRKQSDERLKLEIKVAHIRTRETYGTRRLQTELAENGIIVG
                     RDRLARLRKELRLRCKQKRKFRATTNPNHNLPVAPNLLNQTFAPTAPNQVWVADLTYV
                     ATQEGWLYLAGIKDVYTCEIVGYAMGERMTKELT"
BASE COUNT          116 a          103 c          114 g           74 t
ORIGIN      
        1 gaaccctcag acagaaaaca aagtgatgag cggctaaaac tggagattaa ggtggcacat
       61 atccgcactc gcgaaacata tggaacccgg cggctccaga cggagctggc agagaatggc
      121 atcatcgttg gtcgtgaccg actggcacgt cttcgtaagg agctaaggct acgctgtaag
      181 cagaaacgca agttcagagc gactacgaac ccgaaccaca atctgccagt tgcgccaaat
      241 ctgctgaacc agacgttcgc tcctacagca ccaaatcagg tctgggtggc ggacctgacg
      301 tatgttgcca cacaggaggg atggttgtac ctcgctggca tcaaagatgt ttatacgtgc
      361 gaaattgtcg gctacgccat gggagagcgc atgacaaaag agctgac
//
LOCUS       DAJTMC010000416          402 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.431, whole genome shotgun sequence.
ACCESSION   DAJTMC010000416 DAJTMC010000000
VERSION     DAJTMC010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..402
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.431"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            1..370
                     /gene="leuE"
                     /locus_tag="OO130_004390"
                     /pseudo
     CDS             1..370
                     /gene="leuE"
                     /locus_tag="OO130_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310534.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
BASE COUNT           95 a           77 c          100 g          130 t
ORIGIN      
        1 ggttggggcc atttttattg tgttggtgcc tgggccaaat accctgtttg tactcaaaaa
       61 tagcgtcagt agcggtatga aaggcggtta tcttgcggcc tgcggtgtat ttattggcga
      121 tgcagtattg atgtttctgg catgggctgg agtggcgaca ttaattaaga ccaccccgat
      181 attattcaac attgtacgtt atcttggtgc gttttatttg ctctatctgg agagtaaaat
      241 tctctacgcg accctgaagg gtaaaaataa cgaggccaaa tccgatgagc cccaatacgg
      301 ggccattttt aaacgcgcgt taattttgag cctgactaat ccgaaagcca ttttgttcta
      361 tgtgtcgttt ggtaatgctg ccaacttact gatttagtgt at
//
LOCUS       DAJTMC010000417          401 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.432, whole genome shotgun sequence.
ACCESSION   DAJTMC010000417 DAJTMC010000000
VERSION     DAJTMC010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..401
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.432"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            87..401
                     /locus_tag="OO130_004391"
                     /pseudo
     CDS             87..401
                     /locus_tag="OO130_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415843.2"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
BASE COUNT          129 a           88 c           81 g          103 t
ORIGIN      
        1 atttagggtc taatcaactt aatgatcaat atctatagtg atattaccaa ccgttaaaac
       61 gaaccgacaa ctccatgagg aacggaatga tgaataataa agtcagcttc actaacagca
      121 ataatccaac catctctttg tctgcagtga tctatttccc tccaaaattt gatgaaaccc
      181 gtcagtatca ggcaattgtg gtatctcacc caggtggggc gttaaagaac aaacggccgg
      241 aacctatgcc aaaaaactgg cggaaaaggg atttgtcact attgcttatg acgcatctta
      301 tcaaggtgaa agtggcggcg agccgcgtca gttagaaaac ccatacattc gtactgaaga
      361 catcagtgcc gtgattgatt acctgacaac gctttcttat g
//
LOCUS       DAJTMC010000418          384 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.433, whole genome shotgun sequence.
ACCESSION   DAJTMC010000418 DAJTMC010000000
VERSION     DAJTMC010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..384
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.433"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            186..341
                     /locus_tag="OO130_004392"
                     /pseudo
     CDS             186..341
                     /locus_tag="OO130_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343613.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
BASE COUNT          121 a           84 c           97 g           82 t
ORIGIN      
        1 cagagagcag aacgggatct atacttttgc gggtcaacat aagaggatga gtccttaata
       61 aaacaggtcc tcagagaata catattctgc ggagcgtgcg gcaacaacag gacgaacagg
      121 ggcggttttc aatttccagc cgtcaggctg ccgtggttca ctccaacaac ggagacaggt
      181 aaattatgga cgaaaagaag cttaaagccc ttgcggctga actggctaaa ggcctcaaaa
      241 ccgaggccga cctcaatcag ttttcccgta tgcagacgaa attaactgtc gaaacggtgc
      301 ttaatgagga actaactgac catctcgggc atgaaaaaag ctacatccgg tagtaaacat
      361 tatcagtggg ttatgcgata tgat
//
LOCUS       DAJTMC010000419          372 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.434, whole genome shotgun sequence.
ACCESSION   DAJTMC010000419 DAJTMC010000000
VERSION     DAJTMC010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..372
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.434"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            <1..>372
                     /locus_tag="OO130_004393"
     CDS             <1..>372
                     /locus_tag="OO130_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCR5367505.1"
                     /translation="ETRHAYNAQGLANRCIPDSLPAVEWLTYGSGWLAGMKLGDTPLV
                     DFTRDRLHRETLRRFGRYELSTAYTPAGQLQSQHLNSLQYDRDYTWNDNGELIRISSP
                     RQTRSYSYSTTGRLTGVHTTA"
BASE COUNT           83 a          132 c          105 g           52 t
ORIGIN      
        1 atgagaccag acatgcgtac aacgcgcagg gactggcgaa ccgctgtata ccggacagcc
       61 tgcccgccgt ggaatggctg acctacggca gcggctggct ggcaggtatg aagctcggcg
      121 acacaccgct ggtggatttc acccgcgacc gcctgcaccg ggaaacgctg cgccgcttcg
      181 gccgttatga actctccacc gcttacaccc ctgccgggca gttacagagc cagcacctga
      241 acagcctgca gtatgaccgc gattacacct ggaacgacaa cggcgaactc atccgcatca
      301 gcagcccgcg ccagacccgg agttacagct acagcaccac cggcaggctg accggcgttc
      361 acaccaccgc ag
//
LOCUS       DAJTMC010000420          367 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.435, whole genome shotgun sequence.
ACCESSION   DAJTMC010000420 DAJTMC010000000
VERSION     DAJTMC010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..367
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.435"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            42..239
                     /locus_tag="OO130_004394"
     CDS             42..239
                     /locus_tag="OO130_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005096564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5367506.1"
                     /translation="MRKILQQKGDPDLIQCDYHLCNKQFLPEKAFTGELIYMHGNMIA
                     RKKRKYCGAACAEKDKMAHEL"
BASE COUNT          120 a           67 c           76 g          104 t
ORIGIN      
        1 agaccatcag tcataccggg attaaaaaat ggctgtggag aatcagaaaa attctccagc
       61 aaaaaggtga cccggattta atacaatgtg attaccatct ctgcaataaa cagtttttac
      121 ctgaaaaagc ttttactggc gagcttattt atatgcatgg aaacatgatt gccagaaaga
      181 aacgaaaata ttgcggtgca gcttgtgccg aaaaagacaa gatggcacac gaactttaat
      241 taactgaata ttcgaaactg aatttatgcc agcaatgggg taatgctgcc aacttactaa
      301 tttagtgtat gatggtgatt ttaaggtgct tgcgtggctt ccatttccat cagatgtcct
      361 tcctgct
//
LOCUS       DAJTMC010000421          365 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.436, whole genome shotgun sequence.
ACCESSION   DAJTMC010000421 DAJTMC010000000
VERSION     DAJTMC010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..365
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.436"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(<1..355)
                     /locus_tag="OO130_004395"
     CDS             complement(<1..355)
                     /locus_tag="OO130_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR5367507.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKQARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIK"
BASE COUNT           89 a           82 c           90 g          104 t
ORIGIN      
        1 gcttaatttt gctttcacat ttttgtaagt cttctgccgg attgtgcgac gtctgcaata
       61 tcacgtcgaa accttcctct tcagctttgg cggtgatggc atgcaaaact tcagagaaaa
      121 acggattacc cgccgtagtt ttggtcgaac gggtagaaat caccataatg gcatcaaaac
      181 ccgaagaggt cagcgcgcgg gcctgcttgt ttggctgata ctgtaattct tcaatggccc
      241 gtaacacttt ttcgcgcgct tccggggaga tattagtttg cttattcagc acgcgtgata
      301 cggtggattt tgatacgcct gcaaccctgg caatatcata aatagtaggg gacataggtc
      361 aggaa
//
LOCUS       DAJTMC010000422          361 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.437, whole genome shotgun sequence.
ACCESSION   DAJTMC010000422 DAJTMC010000000
VERSION     DAJTMC010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..361
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.437"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            <1..>361
                     /locus_tag="OO130_004396"
     CDS             <1..>361
                     /locus_tag="OO130_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HCR5367508.1"
                     /translation="KQTMQLSRLTLRSKKPELVEQELWGVLLAYNLVRYQMIKMAEHL
                     KGYWPNQLSFSESCGMVMRMLMTLQGASPGRIPELMRDLASMGQLVKLPTRRGRAFPR
                     VVKERPWKYPTAPKKSQS"
BASE COUNT          108 a           71 c          111 g           71 t
ORIGIN      
        1 aaacagacga tgcaactgag caggctgacg ctgagaagta aaaagccgga gcttgtggag
       61 caagagctgt ggggtgtctt actggcttat aatctggtga gatatcagat gattaaaatg
      121 gcggaacatc tgaaaggtta ctggccgaat caactgagtt tctcagaatc atgcggaatg
      181 gtgatgagaa tgctgatgac attgcagggc gcttcaccgg gacgtatacc ggagctgatg
      241 cgcgatcttg caagtatggg acaacttgtg aaattaccga cgagaagggg aagggccttc
      301 ccgagagtgg taaaggagag gccctggaaa taccccacag ccccgaaaaa gagccagtca
      361 g
//
LOCUS       DAJTMC010000423          349 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.438, whole genome shotgun sequence.
ACCESSION   DAJTMC010000423 DAJTMC010000000
VERSION     DAJTMC010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..349
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.438"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            <1..>349
                     /locus_tag="OO130_004397"
     CDS             <1..>349
                     /locus_tag="OO130_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000878026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCR5367509.1"
                     /translation="GPLIATAAVATMGEASAFKSGREFAAYVGLVPKQTGSGGKVRLL
                     GISKRGDTYLRTLFIHGARAVALVAKEPGPWITELKKRRPASVAIVAMANKLARTVWA
                     ITAHDRKYDRNHVS"
BASE COUNT           95 a           83 c          103 g           68 t
ORIGIN      
        1 tggaccactt attgcgacgg cagcggtggc caccatgggg gaagcatcag cgtttaaatc
       61 ggggcgagag ttcgccgcat atgttggtct ggttccaaaa caaactggct ccggagggaa
      121 agtacgtctg ctggggataa gcaaacgtgg tgacacttat ctcaggacat tatttatcca
      181 cggtgcaaga gcggtggcat tagtagctaa agagcctggc ccgtggataa ccgaactgaa
      241 aaaacgtcgt ccagccagtg tggcaatcgt cgccatggca aacaagctgg cacgaacagt
      301 atgggcgata accgcccatg accgtaagta tgacaggaac cacgtcagt
//
LOCUS       DAJTMC010000424          347 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.439, whole genome shotgun sequence.
ACCESSION   DAJTMC010000424 DAJTMC010000000
VERSION     DAJTMC010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 347)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 347)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..347
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.439"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            <1..164
                     /locus_tag="OO130_004398"
     CDS             <1..164
                     /locus_tag="OO130_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HCR5367510.1"
                     /translation="AGFIKGQSLTLLEKLDALDADEQATMCEKLHELAEELQNSIQTR
                     FEVENSTER"
BASE COUNT          104 a           90 c           97 g           56 t
ORIGIN      
        1 tggcgggatt cataaaaggc cagagcctga cgctgctcga aaaactggac gcactcgatg
       61 ctgacgagca ggccaccatg tgtgagaagc tgcacgaact cgcagaagaa ctccagaaca
      121 gcatacagac acgctttgaa gtagaaaata gcacagagag ataacgctca ggacaggatg
      181 ccgaacggca caacagagcg taaggagaaa atgtcacata aggcgctctt ccgcttcctc
      241 gctcactgac tcgctacgct cggtcgttcg actgcggcga gcggtgtcag ctcactcaaa
      301 ggcggtaatg cggttatcca cagaatcagg ggataacgcc ggaaaga
//
LOCUS       DAJTMC010000425          346 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.440, whole genome shotgun sequence.
ACCESSION   DAJTMC010000425 DAJTMC010000000
VERSION     DAJTMC010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..346
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.440"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            <1..126
                     /locus_tag="OO130_004399"
     CDS             <1..126
                     /locus_tag="OO130_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5367511.1"
                     /translation="ISSLVNSLKGVLWSPGYFASSCGGAPISVIRQYIEQQQTPG"
BASE COUNT          100 a           92 c           69 g           85 t
ORIGIN      
        1 atatccagtc tggtaaacag cctcaaaggc gttttgtgga gtcctggcta ttttgccagt
       61 agctgcggag gtgcgccaat atccgtcatc cgccaataca ttgaacaaca gcaaacacct
      121 ggttaggtgg aaaaccacgc cttatatccc tgccctgaag gacggggttt tacggcgcac
      181 cggataagcc tggagattat ctacaccagc atggacatca ttgtactatg ccccgtacat
      241 gccccatcac atcctcaaca tcaaagtatt agagccttgc aaaccattat gcgtctcagt
      301 ttgtctcatc tatcttacgc aaaaaaaaat agaactaata tcaagc
//
LOCUS       DAJTMC010000426          339 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.441, whole genome shotgun sequence.
ACCESSION   DAJTMC010000426 DAJTMC010000000
VERSION     DAJTMC010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..339
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.441"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            <1..>339
                     /locus_tag="OO130_004400"
     CDS             <1..>339
                     /locus_tag="OO130_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCR5367512.1"
                     /translation="FGIHGALALTALDNGIMTLWALENIATYQQYGSVEAALAAGKTF
                     HIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLALPSGIFQINEPILFG
                     LPIIMNPVMF"
BASE COUNT           63 a           91 c           93 g           92 t
ORIGIN      
        1 tcttcggtat tcatggcgcg ctggcgctga ccgcactgga caacggcatt atgacgctgt
       61 gggcgctgga aaatatcgcg acctatcagc aatatggttc cgtcgaagcg gcgctggcag
      121 ccggtaagac cttccatatc tgggccaagc cgatgctgga ctcctttatt ttccttgggg
      181 gcagtggtgc gactttaggc ctgatcctgg ctatctttat cgcctctcgc cgtgctgatt
      241 atcgtcaggt ggcaaaactg gcgctgccgt ccggcatctt ccagattaac gaaccgattc
      301 tgtttggtct gccaattatc atgaacccgg tgatgttta
//
LOCUS       DAJTMC010000427          334 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.442, whole genome shotgun sequence.
ACCESSION   DAJTMC010000427 DAJTMC010000000
VERSION     DAJTMC010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..334
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.442"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(<1..289)
                     /locus_tag="OO130_004401"
     CDS             complement(<1..289)
                     /locus_tag="OO130_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HCR5367513.1"
                     /translation="MFPDFFMHIGQALDLVSRYDSLRNPLTSLGDYLDPELISRCLAE
                     SGTVTLRKRRLPLEMMVWCIVGMALERKEPLHQIVNRLDIMLPGNRPFVAPS"
BASE COUNT           94 a           80 c           96 g           64 t
ORIGIN      
        1 cactgggggc aacgaagggg cgattgcccg gcagcatgat gtccaggcga ttcacaatct
       61 ggtgaagagg ttctttacgc tcaagcgcca tgccaacaat acaccagacc atcatttcga
      121 ggggaagacg gcgcttgcgt agcgttacag tacctgattc ggcaaggcaa cgagagatga
      181 gttcggggtc gaggtaatcc cccagagaag tcagtgggtt acgcagagaa tcgtaacggg
      241 ataccagatc aagagcctgt ccaatgtgca taaaaaaatc cggaaacaag tgagcgtttc
      301 cggattctta cacagccact ggatcggtca actg
//
LOCUS       DAJTMC010000428          332 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.443, whole genome shotgun sequence.
ACCESSION   DAJTMC010000428 DAJTMC010000000
VERSION     DAJTMC010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.443"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            178..>332
                     /locus_tag="OO130_004402"
     CDS             178..>332
                     /locus_tag="OO130_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR5367514.1"
                     /translation="MKRHNQRLKIISEYFGGVPVKNIGIREVYSFLEIRAAGSKFAIA
                     NQYRALLS"
BASE COUNT          124 a           49 c           74 g           85 t
ORIGIN      
        1 atcggaagag acaaaagaat tgcagttaat gaggctattg cggcaaatat ggaattatta
       61 tccacgcaac atataagctt actggataga ataaaaggta atagcgtaac attattctgc
      121 gagctgatat taaatttcag aaatgaaata gagcgtagac aattaagcac aaacaccatg
      181 aaaagacaca accagaggtt gaaaatcata agtgaatact ttggtggtgt tccggttaaa
      241 aatataggca taagggaggt gtacagcttt cttgaaatca gagcagctgg gagtaaattt
      301 gctattgcta accagtacag ggcgctttta tc
//
LOCUS       DAJTMC010000429          330 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.444, whole genome shotgun sequence.
ACCESSION   DAJTMC010000429 DAJTMC010000000
VERSION     DAJTMC010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..330
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.444"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(<1..>330)
                     /locus_tag="OO130_004403"
     CDS             complement(<1..>330)
                     /locus_tag="OO130_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5367515.1"
                     /translation="LLFLYINKDNIKVIYSLKINQTTPGILVSCDSNNNFACQTTMTE
                     DVIQRITTFFHTSPDVKNREIKLEWSGDKRDLPTAEAEISRVQASIIKWYASEYHNGR
                     QVLDEIQ"
BASE COUNT           85 a           67 c           59 g          119 t
ORIGIN      
        1 atttgtatct catcgagaac ttgcctgcca ttatggtatt ctgacgcata ccatttgata
       61 atagacgcct gcacgcgaga tatttccgct tcagcagttg gtaaatctct cttatctcct
      121 gaccattcca gctttatttc tctgttcttg acatctgggc tggtgtgaaa aaatgtagta
      181 attcgctgta taacatcttc agtcattgtg gtctgacagg caaaattatt attgctatca
      241 cagctaacaa gtatacctgg cgttgtctgg tttattttta ggctgtaaat cacttttata
      301 ttgtctttat tgatatacag aaacaacagc
//
LOCUS       DAJTMC010000430          326 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.445, whole genome shotgun sequence.
ACCESSION   DAJTMC010000430 DAJTMC010000000
VERSION     DAJTMC010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..326
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.445"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            <1..>326
                     /locus_tag="OO130_004404"
     CDS             <1..>326
                     /locus_tag="OO130_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR5367516.1"
                     /translation="EYPQSAAIDLRCVNMVADLWHAPAPKNGQAVGTNTIGSSEACML
                     GGMAMKWRWRKRMEAAGKPTDKPNLVCGPVQICWHKFARYWDVELREIPMRPGQLFMD
                     PKRMIEA"
BASE COUNT           74 a           85 c           97 g           70 t
ORIGIN      
        1 gaatatccgc aatccgcagc catcgacctg cgttgcgtaa atatggttgc cgatctgtgg
       61 catgcgcctg cgccgaaaaa tggtcaggcc gttggcacca acaccattgg ttcttccgag
      121 gcctgtatgc tcggcgggat ggcgatgaaa tggcgttggc gcaagcgtat ggaagctgca
      181 ggcaaaccaa cggataaacc aaacctggtg tgcggtccgg tacaaatctg ctggcataaa
      241 ttcgcccgct actgggatgt ggagctgcgt gagatcccta tgcgccccgg tcagttgttt
      301 atggacccga aacgcatgat tgaagc
//
LOCUS       DAJTMC010000431          318 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.446, whole genome shotgun sequence.
ACCESSION   DAJTMC010000431 DAJTMC010000000
VERSION     DAJTMC010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..318
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.446"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            <1..>318
                     /locus_tag="OO130_004405"
     CDS             <1..>318
                     /locus_tag="OO130_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HCR5367517.1"
                     /translation="SFSPDSRLTMKALEMAWETRGKPGGVMFHSDQGSHYTSRQFRQL
                     LWRYQIRQSMSRRGNCWDNSPMERFFRSLKNEWMPVVGYVSFSEAAHAITDYIVGYYS
                     ALRP"
BASE COUNT           82 a           76 c           99 g           61 t
ORIGIN      
        1 gtcgttctcg ccggacagca ggctcaccat gaaagcgctg gaaatggcat gggaaacccg
       61 tggtaagccc ggcggggtga tgttccacag cgatcagggc agtcattata cgagcaggca
      121 gttccggcag ttattgtggc gataccagat cagacagagt atgagccggc gcggaaactg
      181 ctgggataac agcccaatgg aacgcttctt caggagtctg aagaacgaat ggatgccggt
      241 ggtgggttac gtaagcttca gcgaggcagc tcacgccata acggactata tcgttggata
      301 ttacagcgca ctaagacc
//
LOCUS       DAJTMC010000432          318 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.447, whole genome shotgun sequence.
ACCESSION   DAJTMC010000432 DAJTMC010000000
VERSION     DAJTMC010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..318
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.447"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            <1..>318
                     /locus_tag="OO130_004406"
     CDS             <1..>318
                     /locus_tag="OO130_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HCR5367518.1"
                     /translation="YPALVGNDIGCGMALWQTDILARKYNADKFEKRLSDLDDVAEES
                     WLEENLPSAFAQHPWCSSLGSIGGGNHFAELQQVDQIINAELFALAGLDAQHLQLLVH
                     SGS"
BASE COUNT           80 a           76 c           83 g           79 t
ORIGIN      
        1 tttatccggc actggtcggc aatgatatcg gctgcggtat ggcgctatgg caaacagata
       61 ttctcgctcg caaatacaac gccgataagt ttgaaaagcg attatcagat ctggatgacg
      121 ttgctgaaga aagctggctg gaggaaaacc tgccgtcagc atttgcacag catccgtggt
      181 gcagctcgct tggctctatc ggtggcggca atcatttcgc tgaactgcaa caagttgatc
      241 aaatcatcaa cgctgaactg tttgcactag caggtctgga tgcacaacat ctgcaactgt
      301 tggttcatag cggctcgc
//
LOCUS       DAJTMC010000433          296 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.448, whole genome shotgun sequence.
ACCESSION   DAJTMC010000433 DAJTMC010000000
VERSION     DAJTMC010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..296
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.448"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(<1..>296)
                     /locus_tag="OO130_004407"
     CDS             complement(<1..>296)
                     /locus_tag="OO130_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCR5367519.1"
                     /translation="SVRLTQSTRRLSEIGVIRVYEDSTDGSLNDFLGATNIDLRPESL
                     KKFEELAQQAQQSAGSAAGNARQTAQDMTAAATARDDAQRFAEKARQDATVTAE"
BASE COUNT           49 a           89 c           80 g           78 t
ORIGIN      
        1 ctcagctgtg acggttgcat cctgtcgggc tttctccgca aaacgttgtg catcatcacg
       61 ggccgttgcg gctgccgtca tatcctgcgc cgtctgccgt gcgtttccgg ctgcgctccc
      121 ggcactctgc tgcgcctgtt gcgccagctc ttcgaatttc ttcagggatt ccggacgcag
      181 gtcaatatta gttgcgccca gaaaatcatt cagcgaaccg tccgttgaat cttcataaac
      241 gcggatgaca ccgatttctg aaaggcgccg tgtgctttgt gtcaggcgaa cgctgt
//
LOCUS       DAJTMC010000434          285 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.449, whole genome shotgun sequence.
ACCESSION   DAJTMC010000434 DAJTMC010000000
VERSION     DAJTMC010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..285
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.449"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            46..>285
                     /locus_tag="OO130_004408"
     CDS             46..>285
                     /locus_tag="OO130_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCR5367520.1"
                     /translation="MSVVISGALTDGAGIPMSGYHIILKSRVNTPEVVMHTVADVMTG
                     NDGEYCFHARTGKYGVYLKPGWHNEYNVGDIAVYED"
BASE COUNT           75 a           59 c           75 g           76 t
ORIGIN      
        1 ctctccggaa atttcttcct gatttaattt atcgcgagaa aaactatgtc tgtagtgata
       61 tcaggtgcgc tgactgatgg cgcaggtatc cccatgtccg gataccatat tattctgaaa
      121 tcccgggtaa acaccccgga agtggtgatg cacactgttg ctgatgtgat gacaggaaac
      181 gatggtgaat actgtttcca tgcgcggact ggaaaatatg gtgtgtatct gaaaccgggc
      241 tggcacaacg agtacaacgt tggcgacatt gctgtatatg aggac
//
LOCUS       DAJTMC010000435          283 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.450, whole genome shotgun sequence.
ACCESSION   DAJTMC010000435 DAJTMC010000000
VERSION     DAJTMC010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..283
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.450"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            1..283
                     /locus_tag="OO130_004409"
                     /pseudo
     CDS             1..283
                     /locus_tag="OO130_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT           61 a           71 c           87 g           64 t
ORIGIN      
        1 ctcctcaaag aagccgttga atatggacgg gcaaaaaagt ggatagcgca cgcgccctta
       61 ttgcccgggg atggggagta agcttagtca gccgttgtct ccgggtgtcg cgtgcgcagt
      121 tgcacgtcat tctcagacga accgatgact ggatggatgg ccgccgcagt cgtcacactg
      181 atgatacgga tgtgcttctc cgtatacacc atgttatcgg agagctgccc acgtatggtt
      241 atcgtcgggt atgggcgctg cttcgcagac aggcagaact tga
//
LOCUS       DAJTMC010000436          278 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.451, whole genome shotgun sequence.
ACCESSION   DAJTMC010000436 DAJTMC010000000
VERSION     DAJTMC010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..278
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.451"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            24..100
                     /locus_tag="OO130_004410"
     tRNA            24..100
                     /locus_tag="OO130_004410"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:58..60,aa:Ile,seq:gat)
     gene            143..218
                     /locus_tag="OO130_004411"
     tRNA            143..218
                     /locus_tag="OO130_004411"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:176..178,aa:Ala,seq:tgc)
BASE COUNT           76 a           61 c           73 g           68 t
ORIGIN      
        1 aaaatgagca gtaaaacctc tacaggcttg tagctcaggt ggttagagcg cacccctgat
       61 aagggtgagg tcggtggttc aagtccactc aggcctacca aatttgcacg gcaaatttga
      121 agaggtttta actacatgtt atggggctat agctcagctg ggagagcgcc tgctttgcac
      181 gcaggaggtc tgcggttcga tcccgcatag ctccaccatc tctgtagtga ttaaataaaa
      241 aatacttcag agtgtacctg aaaaggttca ctgcgaag
//
LOCUS       DAJTMC010000437          278 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.452, whole genome shotgun sequence.
ACCESSION   DAJTMC010000437 DAJTMC010000000
VERSION     DAJTMC010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..278
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.452"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            <1..>278
                     /locus_tag="OO130_004412"
     CDS             <1..>278
                     /locus_tag="OO130_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5367521.1"
                     /translation="DIHLHPKIGADWQLRGQQKRVVTPGQNEKYYLAGALHSGTGKVS
                     YVGGNSKSSALFISLLKRLKATYRRAKTITLIVDNYIIHKSRETQSWL"
BASE COUNT           82 a           71 c           79 g           46 t
ORIGIN      
        1 ggatatccat cttcatccca aaatcggtgc ggactggcaa ctgcgcggac agcaaaaacg
       61 ggtggtcacg ccgggacaga atgaaaaata ttatctggcc ggagcgctgc acagcgggac
      121 aggtaaagtc agctatgtgg gcggcaacag caaaagttcg gcgctgttca tcagcctgct
      181 gaagcggctt aaagcgacat accgtcgggc gaaaaccatc acgctgatcg tggacaacta
      241 cattatccac aaaagccggg aaacacagag ctggctga
//
LOCUS       DAJTMC010000438          277 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.453, whole genome shotgun sequence.
ACCESSION   DAJTMC010000438 DAJTMC010000000
VERSION     DAJTMC010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..277
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.453"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            109..>277
                     /locus_tag="OO130_004413"
     CDS             109..>277
                     /locus_tag="OO130_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCR5367522.1"
                     /translation="MNNNNTLYVGLDVHKESITVAYAINSEPVELMGKIGTSPTDIQN
                     LCKRLRSKSSQV"
BASE COUNT           79 a           62 c           65 g           71 t
ORIGIN      
        1 gagacagatg ctggccaacg tgggatattt aacgcgggtt aacatcgttg tcagagccgc
       61 atgggtgagc tgttgctaca ccccggtatt tccaccacgg aggccagcat gaacaacaat
      121 aacacattgt acgtcgggtt agatgttcac aaagaatcga ttactgtcgc ttatgctatc
      181 aattcagaac ctgttgaact gatgggtaaa attggcacat cacctactga tattcagaat
      241 ctttgtaaac gtctcaggtc aaagtcatcg caggtca
//
LOCUS       DAJTMC010000439          272 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.454, whole genome shotgun sequence.
ACCESSION   DAJTMC010000439 DAJTMC010000000
VERSION     DAJTMC010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..272
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.454"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(<1..>272)
                     /locus_tag="OO130_004414"
     CDS             complement(<1..>272)
                     /locus_tag="OO130_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCR5367523.1"
                     /translation="DLARAWASARDDLRHARQRLKSFLLVHGVHYVGRADWGPAHRRW
                     LSKYSFESPWRQLAFDEHRRTIEDRQAQCERLESALKEAVTEWRLYP"
BASE COUNT           59 a           79 c           71 g           63 t
ORIGIN      
        1 ggatacagcc tccattcggt aacggcctcc ttcagggcgg attccagccg ttcacattgt
       61 gcctgccggt cttctattgt acgacggtgt tcgtcaaaag ctaattgtcg ccagggactt
      121 tcgaatgagt atttgctgag ccagcgtcgg tgtgcagggc cccaatctgc tcgcccgaca
      181 taatggaccc catgaaccag aagaaacgat ttcagacgtt gccttgcatg ccgtaaatca
      241 tcgcgggcag atgcccatgc ccgggccaaa tc
//
LOCUS       DAJTMC010000440          257 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.455, whole genome shotgun sequence.
ACCESSION   DAJTMC010000440 DAJTMC010000000
VERSION     DAJTMC010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..257
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.455"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
BASE COUNT           56 a           90 c           56 g           55 t
ORIGIN      
        1 cacagaccgg actgactccg gaaaaacaga ggcccgcccc cgggcctccc cggattcatc
       61 cgtttccctg ttcagcctga cagcacgccc ccgcggccgg atgacagact ccgcttcggt
      121 aagcaaagcg gtcttctgtg attccgccag ttgcggctta ttcattactc aacgtcaaac
      181 gcccgaattg aagccaaatc atccagaccg ctcagctcct ctttcatttc acgctgacgg
      241 cgataaatct catcgtt
//
LOCUS       DAJTMC010000441          256 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.456, whole genome shotgun sequence.
ACCESSION   DAJTMC010000441 DAJTMC010000000
VERSION     DAJTMC010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 256)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 256)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..256
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.456"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
BASE COUNT           73 a           65 c           69 g           49 t
ORIGIN      
        1 tcatttggca tcagtgagga ttgtatgaaa agtgcaccat gccgggtgtg aaatgccgca
       61 cagatgcgta aggagaaaat gctcgtccag gcgcttttcc gcttcctcgc tcactgactc
      121 gctccgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaaga cggtaatgcg
      181 gttatccaca gaatcagggg ataacaccaa aagaaacatg tgagcaaaaa acaagaaccc
      241 ggaaaaggcc acgcag
//
LOCUS       DAJTMC010000442          252 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.457, whole genome shotgun sequence.
ACCESSION   DAJTMC010000442 DAJTMC010000000
VERSION     DAJTMC010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.457"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            <1..252
                     /locus_tag="OO130_004415"
     rRNA            <1..252
                     /locus_tag="OO130_004415"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           58 a           60 c           75 g           59 t
ORIGIN      
        1 gtgcgtcgta gtccggattg gagtctgcaa ctcgactcca tgaagtcgga atcgctagta
       61 atcgtggatc agaatgccac ggtgaatacg ttcccgggcc ttgtacacac cgcccgtcac
      121 accatgggag tgggttgcaa aagaagtagg tagcttaacc ttcgggaggg cgcttaccac
      181 tttgtgattc atgactgggg tgaagtcgta acaaggtaac cgtaggggaa cctgcggttg
      241 gatcacctcc tt
//
LOCUS       DAJTMC010000443          251 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.458, whole genome shotgun sequence.
ACCESSION   DAJTMC010000443 DAJTMC010000000
VERSION     DAJTMC010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..251
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.458"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(<1..95)
                     /locus_tag="OO130_004416"
     CDS             complement(<1..95)
                     /locus_tag="OO130_004416"
                     /inference="COORDINATES: protein motif:HMM:NF019996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCR5367524.1"
                     /translation="MTVKISGVLKDGTGKPVQNCTIQLKAKRNSTT"
     gene            complement(160..>251)
                     /locus_tag="OO130_004417"
     CDS             complement(160..>251)
                     /locus_tag="OO130_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HCR5367525.1"
                     /translation="SVAIDIAYEGSGSGDWRTDGFIVGVGYKF"
BASE COUNT           59 a           70 c           54 g           68 t
ORIGIN      
        1 gtggtactgt tacgttttgc tttcagctgg attgtgcagt tctgtaccgg ttttcctgtg
       61 ccgtctttca gtacaccaga aatctttact gtcatattca ccccacaaaa aagcccgcct
      121 gaaccggcgg gctgtcatga cactgtgtta cctggctaat cagaatttat agccgacacc
      181 cacgatgaaa ccgtcagtgc gccagtcgcc actgccggaa ccttcataag caatatcaat
      241 ggccacggat t
//
LOCUS       DAJTMC010000444          247 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.459, whole genome shotgun sequence.
ACCESSION   DAJTMC010000444 DAJTMC010000000
VERSION     DAJTMC010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..247
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.459"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
BASE COUNT           80 a           51 c           64 g           52 t
ORIGIN      
        1 taccattaaa caatgaactc ttaacaaaag ggtgaatgct gaaaggttgc tatggcggcc
       61 agagtgatga caaagacagg taagaccgtg actcactaaa cctgaacagt attttgggct
      121 tgaagtccgc cgtgaaaata aggggtgagt cggcgaatta cataggggct cgcagcgtta
      181 cggctgcaat aaagccggat ataaagctgc aacctacccg tcatgtcaaa acaatggatg
      241 ccttgca
//
LOCUS       DAJTMC010000445          245 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.460, whole genome shotgun sequence.
ACCESSION   DAJTMC010000445 DAJTMC010000000
VERSION     DAJTMC010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..245
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.460"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(<1..>245)
                     /locus_tag="OO130_004418"
     CDS             complement(<1..>245)
                     /locus_tag="OO130_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HCR5367526.1"
                     /translation="IPLDDALLQLREFIDENSGEFFVQVWGNGANFDNTILRRSYERQ
                     GIPCPWRYYNDRDVRTIVELGKAIDFDARTAIPFEGER"
BASE COUNT           59 a           66 c           58 g           62 t
ORIGIN      
        1 cgctcacctt cgaatggaat agccgttctg gcatcgaagt ctatggcttt ccccagctca
       61 acgattgtgc gtacatcgcg atcgttgtag taacgccacg ggcaggggat cccctgccgt
      121 tcgtatgaac ggcgcaaaat cgtgttgtcg aagttggctc catttcccca gacctgaaca
      181 aaaaattcac cggagttttc gtcgataaat tcccgcaatt gtaacagtgc atcatctaac
      241 gggat
//
LOCUS       DAJTMC010000446          240 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.461, whole genome shotgun sequence.
ACCESSION   DAJTMC010000446 DAJTMC010000000
VERSION     DAJTMC010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..240
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.461"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            3..>240
                     /locus_tag="OO130_004419"
     CDS             3..>240
                     /locus_tag="OO130_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004136079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR5367527.1"
                     /translation="MLRKVEKIYDNGFKPVHGIDLDINDGEFMVFVGPSGCAKSTTLR
                     MIAGLEDVSGGEIYISDRLVNTLPPKDRSIAMVFQ"
BASE COUNT           70 a           47 c           51 g           72 t
ORIGIN      
        1 taattctacg caaagttgaa aaaatttatg acaatggatt caaacctgtt cacggcattg
       61 atctcgatat aaacgatggt gaattcatgg tttttgttgg accatctggg tgtgccaaat
      121 caactacatt acgcatgatt gcaggtcttg aagacgtatc aggtggtgaa atttacatta
      181 gcgaccgcct tgttaatacc cttccgccta aagatcgcag cattgcaatg gtgttccaga
//
LOCUS       DAJTMC010000447          237 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.462, whole genome shotgun sequence.
ACCESSION   DAJTMC010000447 DAJTMC010000000
VERSION     DAJTMC010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..237
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.462"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            5..>237
                     /locus_tag="OO130_004420"
     CDS             5..>237
                     /locus_tag="OO130_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5367528.1"
                     /translation="MSRKNQRYSKEFKAEAVRTVLENQLSISEGASRLSLPEGTLGQW
                     VTAARKGLGTPGSRTVAELESEILQLRKALNEAR"
BASE COUNT           70 a           56 c           57 g           54 t
ORIGIN      
        1 aataatgagc agaaaaaacc aacgttactc taaagagttc aaagccgaag ctgtcagaac
       61 ggttcttgaa aatcaacttt cgatcagtga aggcgcttcc cgattatccc ttcctgaagg
      121 cactttagga caatgggtta ccgccgccag aaaagggctc ggtactcctg gttcccgcac
      181 ggtggctgaa ctggaatctg aaattctgca actgcgtaag gcgttaaatg aagctcg
//
LOCUS       DAJTMC010000448          236 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.463, whole genome shotgun sequence.
ACCESSION   DAJTMC010000448 DAJTMC010000000
VERSION     DAJTMC010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..236
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.463"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            <1..>236
                     /locus_tag="OO130_004421"
     CDS             <1..>236
                     /locus_tag="OO130_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HCR5367529.1"
                     /translation="LLSAFEVVVWMTDGWPLYESRLKGKLHVISKRYTQRIERHNLNL
                     RQHLARLGRKSLSFSKSVELHDKVIGHYLNIKHY"
BASE COUNT           62 a           53 c           67 g           54 t
ORIGIN      
        1 gcctgctgtc ggcctttgag gtcgtggtat ggatgacgga tggctggccg ctgtatgaat
       61 cacgcctgaa gggaaagctg cacgttatca gcaagcgtta cactcagcgc attgagcgac
      121 ataatctgaa tctgagacaa catctggcaa ggctgggacg gaagtcactg tcgttctcaa
      181 aatcggtgga gctgcatgac aaggtcatcg ggcattatct gaacataaaa cactat
//
LOCUS       DAJTMC010000449          235 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.464, whole genome shotgun sequence.
ACCESSION   DAJTMC010000449 DAJTMC010000000
VERSION     DAJTMC010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..235
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.464"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            <1..>235
                     /locus_tag="OO130_004422"
     CDS             <1..>235
                     /locus_tag="OO130_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HCR5367530.1"
                     /translation="HWMIPLRKGAQYEELRKLGKGDHLVKLKTSPQARKKWPGLGNEV
                     TARLLTVTRKGKVCHLLTSMTDAMRFPGGEMADL"
BASE COUNT           69 a           58 c           73 g           35 t
ORIGIN      
        1 gccactggat gatacctctc agaaagggag cgcaatatga agagctcaga aaactgggta
       61 aaggcgatca tctggtgaag ctgaaaacca gcccgcaggc acgaaaaaag tggccgggac
      121 tgggaaatga agtgacagcc cgcctgctga ccgtgacgcg caaaggaaaa gtctgccatc
      181 tgctgacgtc gatgacggac gccatgcgct tccccggagg agaaatggcg gatct
//
LOCUS       DAJTMC010000450          232 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.465, whole genome shotgun sequence.
ACCESSION   DAJTMC010000450 DAJTMC010000000
VERSION     DAJTMC010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..232
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.465"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(<1..73)
                     /gene="csgC"
                     /locus_tag="OO130_004423"
     CDS             complement(<1..73)
                     /gene="csgC"
                     /locus_tag="OO130_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="involved in autoagglutination of curliated cells;
                     not involved in production of curli fibers; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HCR5367531.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDV"
     gene            complement(132..>232)
                     /locus_tag="OO130_004424"
     CDS             complement(132..>232)
                     /locus_tag="OO130_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="curlin"
                     /protein_id="HCR5367532.1"
                     /translation="GGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
BASE COUNT           63 a           55 c           55 g           59 t
ORIGIN      
        1 acacatcccc ttgctgggtc gtattaaagg ttatctgact ggaaagtgcc gcaaggagta
       61 ataacgcatt catattcttc tcccgaaaaa aaacagggct tgcgccctgt ttctgtaata
      121 caaatgatgt attagtactg atgagcggtc gcgttgttac caaagccaac ctgagtcacg
      181 ttgacggagg agttagatgc agtctggtca actgcagcac cgttgccacc ac
//
LOCUS       DAJTMC010000451          232 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.denovo.466, whole genome shotgun sequence.
ACCESSION   DAJTMC010000451 DAJTMC010000000
VERSION     DAJTMC010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..232
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.denovo.466"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            complement(<1..207)
                     /locus_tag="OO130_004425"
     CDS             complement(<1..207)
                     /locus_tag="OO130_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5367533.1"
                     /translation="MIVLILVFRLVIGVQMIDVLGPEKRRRRTTQEKIAIVQQSFEPG
                     MTVSLVARQHGVAASQLFLWRKQYQ"
BASE COUNT           56 a           58 c           51 g           67 t
ORIGIN      
        1 ctggtattgc ttacgccaga gaaataactg gctggctgct acaccatgtt gccgggcaac
       61 gagggagacc gtcatccccg gttcaaagct ctgctgaaca attgcgatct tttcctgtgt
      121 ggtacgccgt ctgcgtttct ccggccctaa gacatcaatc atctgtactc caatgactag
      181 tctaaaaact agtattaaga ctatcactta tttaagtgat actggttgtc tg
//
LOCUS       DAJTMC010000452         3976 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.guided.243, whole genome shotgun sequence.
ACCESSION   DAJTMC010000452 DAJTMC010000000
VERSION     DAJTMC010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3976
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.guided.243"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            980..1795
                     /gene="sul2"
                     /locus_tag="OO130_004426"
     CDS             980..1795
                     /gene="sul2"
                     /locus_tag="OO130_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HCR5367534.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            1856..2383
                     /locus_tag="OO130_004427"
                     /pseudo
     CDS             1856..2383
                     /locus_tag="OO130_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
     gene            2404..2877
                     /locus_tag="OO130_004428"
     CDS             2404..2877
                     /locus_tag="OO130_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA14"
                     /protein_id="HCR5367535.1"
                     /translation="MKVSLIAAKAKNGVIGCGPDIPWSAKGEQLLFKALTYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSGWTSNDDNVVVFQSIEEAMDRLAEFTGHVIVSGGGEI
                     YRETLPMASTLHLSTIDIEPEGDVFFPSIPNTFEVVFEQHFTSNINYCYQIWKKG"
     gene            2952..3227
                     /locus_tag="OO130_004429"
                     /pseudo
     CDS             2952..3227
                     /locus_tag="OO130_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase APH(3')"
     gene            3227..>3976
                     /locus_tag="OO130_004430"
     CDS             3227..>3976
                     /locus_tag="OO130_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APH(6)-I family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HCR5367536.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLS"
BASE COUNT          880 a         1069 c         1071 g          956 t
ORIGIN      
        1 tgactcgcta cgctcggtcg ttcggctgcg gcgagcggta ccggcttacg gacggggacg
       61 gaaatttcct ggaagatacc aggaagacac ttagcaaaga agagataagg ccacggcgaa
      121 gccgtttttc cataggctcc gcccccctga caagcatcgc aaaatctgac gctcaaatca
      181 gtggtggcga aacccgacag gactataaag atcccaggcg tttccccctg gtagctccct
      241 cgtgcgctct cctgttcctg cctttcggtt taccggtgtc attccgctgt tatggccgcg
      301 gtttctcatt ccacgcctga cactcagttc cgtgaaggca gttcgctcca agctggactg
      361 tatgcacgaa ccccccgttc agtccgaccg ctgcgccttg tccggtaact atcaacttga
      421 gtccaacccg gaaagacacg acaaaacgcc actggcagca gccattagta acaggagtgc
      481 acgagaagat agattaagag gtcttgaagt agaggcctga ctgcggctac actgaaagaa
      541 cagttttggt aatggcggtc tcgtgaaccc tgttaccggg ttaagcagtt ccccaactga
      601 cttaaccttc gaacaaaccg cctccccagg cggttttttc gtttacagag caagagatta
      661 cgattcattg aaaaggatct caagaagatc ctttgtagat tactatcaat taatcttccg
      721 aacatgacac cagtcactgt tgattacggt taaaccgctc cgtaacaggt ttccctcgta
      781 ttggtgactt gcagcaccaa agaagcaaaa aagataaata tatctactta ttgagagatg
      841 atatatttat ttccaaaatg ctacagatac atcatcatga aacggatcac cgcaaacagg
      901 ttactcgcct ggggattccc tttcgacccg agcatccgta tgagactcat gctcgattat
      961 tattattata gaagccccca tgaataaatc gctcatcatt ttcggcatcg tcaacataac
     1021 ctcggacagt ttctccgatg gaggccggta tctggcgcca gacgcagcca ttgcgcaggc
     1081 gcgtaagctg atggccgagg gggcagatgt gatcgacctc ggtccggcat ccagcaatcc
     1141 cgacgccgcg cctgtttcgt ccgacacaga aatcgcgcgt atcgcgccgg tgctggacgc
     1201 gctcaaggca gatggcattc ccgtctcgct cgacagttat caacccgcga cgcaagccta
     1261 tgccttgtcg cgtggtgtgg cctatctcaa tgatattcgc ggttttccag acgctgcgtt
     1321 ctatccgcaa ttggcgaaat catctgccaa actcgtcgtt atgcattcgg tgcaagacgg
     1381 gcaggcagat cggcgcgagg cacccgctgg cgacatcatg gatcacattg cggcgttctt
     1441 tgacgcgcgc atcgcggcgc tgacgggtgc cggtatcaaa cgcaaccgcc ttgtccttga
     1501 tcccggcatg gggttttttc tgggggctgc tcccgaaacc tcgctctcgg tgctggcgcg
     1561 gttcgatgaa ttgcggctgc gcttcgattt gccggtgctt ctgtctgttt cgcgcaaatc
     1621 ctttctgcgc gcgctcacag gccgtggtcc gggggatgtc ggggccgcga cactcgctgc
     1681 agagcttgcc gccgccgcag gtggagctga cttcatccgc acacacgagc cgcgcccctt
     1741 gcgcgacggg ctggcggtat tggcggcgct gaaagaaacc gcaagaattc gttaactgca
     1801 cattcgggat atttctctat attcgcgctt catcagaaaa ctgaaggaac ctccattgaa
     1861 tcgaactaat attttttttg gtgaatcgca ttctgactgg ttgcctgtca gaggcggaga
     1921 atctggtgat tttgtttttc gacgtggtga cgggcatgcc ttcgcgaaaa tcgcacctgc
     1981 ttcccgccgc ggtgagctcg ctggagagcg tgaccgcctc atttggctca aaggtcgagg
     2041 tgtggcttgc cccgaggtca tcaactggca ggaggaacag gagggtgcat gcttggtgat
     2101 aacggcaatt ccgggagtac cggcggctga tctgtctgga gcggatttgc tcaaagcgtg
     2161 gccgtcaatg gggcagcaac ttggcgctgt tcacagccta tcggttgatc aatgtccgtt
     2221 tgagcgcagg ctgtcgcgaa tgttcggacg cgccgttgat gtggtgtccc gcaatgccgt
     2281 caatcccgac ttcttaccgg acgaggacaa gagtacgccg cagctcgatc ttttggctcg
     2341 tgtcgaacta gagctaccgg tgcggctcga ccaagagcgc accgttaacc caggatgaga
     2401 accttgaaag tatcattgat agctgcgaaa gcgaaaaacg gcgtgattgg ttgcggtcca
     2461 gacataccct ggtccgcgaa aggggagcag ctacttttta aagcattgac ctacaatcag
     2521 tggcttctgg tgggtcgcaa gacgtttgaa tctatgggcg cactccccaa taggaaatac
     2581 gcggtcgtta cccgctcagg ttggacatca aatgatgaca atgtagttgt atttcagtca
     2641 atcgaagagg ccatggacag gctagctgaa ttcaccggtc acgttatagt gtctggtggc
     2701 ggagaaattt accgagaaac attacccatg gcctctacgc tccacttatc gacgatcgac
     2761 atcgagccag agggggatgt tttcttcccg agtattccaa ataccttcga agttgttttt
     2821 gagcaacact ttacttcaaa cattaactat tgctatcaaa tttggaaaaa gggttaacaa
     2881 agctatgcaa tcgacggcaa aaagcttcgt tcgcttcgcg cactacgcct ttttccgcga
     2941 ttgatagcga cgatatggtt gtttgccatg gtgatccctg catgccgaac ttcatggtgg
     3001 accctaaaac tcttcaatgc acgggtctga tcgaccttgg gcggctcgga acagcagatc
     3061 gctatgccga tttggcactc atgattgcta acgccgaaga gaactgggca gcgccagatg
     3121 aagcagagcg cgccttcgct gtcctattca atgtattggg gatcgaagcc cccgaccgcg
     3181 aacgccttgc cttctatctg cgattggacc ctctgacttg gggttgatgt tcatgccgcc
     3241 tgtttttcct gctcattggc acgtttcgca acctgttctc attgcggaca ccttttccag
     3301 cctcgtttgg aaagtttcat tgccagacgg gactcctgca atcgtcaagg gattgaaacc
     3361 tatagaagac attgctgatg aactgcgcgg ggccgactat ctggtatggc gcaatgggag
     3421 gggagcagtc cggttgctcg gtcgtgagaa caatctgatg ttgctcgaat atgccgggga
     3481 gcgaatgctc tctcacatcg ttgccgagca cggcgactac caggcgaccg aaattgcagc
     3541 ggaactaatg gcgaagctgt atgccgcatc tgaggaaccc ctgccttctg cccttctccc
     3601 gatccgggat cgctttgcag ctttgtttca gcgggcgcgc gatgatcaaa acgcaggttg
     3661 tcaaactgac tacgtccacg cggcgattat agccgatcaa atgatgagca atgcctcgga
     3721 actgcgtggg ctacatggcg atctgcatca tgaaaacatc atgttctcca gtcgcggctg
     3781 gctggtgata gatcccgtcg gtctggtcgg tgaagtgggc tttggcgccg ccaatatgtt
     3841 ctacgatccg gctgacagag acgacctttg tctcgatcct agacgcattg cacagatggc
     3901 ggacgcattc tctcgtgcgc tggacgtcga tccgcgtcgc ctgctcgacc aggcgtacgc
     3961 ttatgggtgc ctttcc
//
LOCUS       DAJTMC010000453         3770 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.guided.248, whole genome shotgun sequence.
ACCESSION   DAJTMC010000453 DAJTMC010000000
VERSION     DAJTMC010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3770
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.guided.248"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            96..569
                     /locus_tag="OO130_004431"
     CDS             96..569
                     /locus_tag="OO130_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA14"
                     /protein_id="HCR5367537.1"
                     /translation="MKVSLIAAKAKNGVIGCGPDIPWSAKGEQLLFKALTYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSGWTSNDDNVVVFQSIEEAMDRLAEFTGHVIVSGGGEI
                     YRETLPMASTLHLSTIDIEPEGDVFFPSIPNTFEVVFEQHFTSNINYCYQIWKKG"
     gene            644..919
                     /locus_tag="OO130_004432"
                     /pseudo
     CDS             644..919
                     /locus_tag="OO130_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase APH(3')"
     gene            919..1755
                     /locus_tag="OO130_004433"
     CDS             919..1755
                     /locus_tag="OO130_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HCR5367538.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(1862..2266)
                     /locus_tag="OO130_004434"
                     /pseudo
     CDS             complement(1862..2266)
                     /locus_tag="OO130_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(2310..2699)
                     /locus_tag="OO130_004435"
     CDS             complement(2310..2699)
                     /locus_tag="OO130_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5367539.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            2917..3357
                     /locus_tag="OO130_004436"
     CDS             2917..3357
                     /locus_tag="OO130_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HCR5367540.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
BASE COUNT          955 a          980 c          965 g          870 t
ORIGIN      
        1 aagagtacgc cgcagctcga tcttttggct cgtgtcgaac tagagctacc ggtgcggctc
       61 gaccaagagc gcaccgttaa cccaggatga gaaccttgaa agtatcattg atagctgcga
      121 aagcgaaaaa cggcgtgatt ggttgcggtc cagacatacc ctggtccgcg aaaggggagc
      181 agctactttt taaagcattg acctacaatc agtggcttct ggtgggtcgc aagacgtttg
      241 aatctatggg cgcactcccc aataggaaat acgcggtcgt tacccgctca ggttggacat
      301 caaatgatga caatgtagtt gtatttcagt caatcgaaga ggccatggac aggctagctg
      361 aattcaccgg tcacgttata gtgtctggtg gcggagaaat ttaccgagaa acattaccca
      421 tggcctctac gctccactta tcgacgatcg acatcgagcc agagggggat gttttcttcc
      481 cgagtattcc aaataccttc gaagttgttt ttgagcaaca ctttacttca aacattaact
      541 attgctatca aatttggaaa aagggttaac aaagctatgc aatcgacggc aaaaagcttc
      601 gttcgcttcg cgcactacgc ctttttccgc gattgatagc gacgatatgg ttgtttgcca
      661 tggtgatccc tgcatgccga acttcatggt ggaccctaaa actcttcaat gcacgggtct
      721 gatcgacctt gggcggctcg gaacagcaga tcgctatgcc gatttggcac tcatgattgc
      781 taacgccgaa gagaactggg cagcgccaga tgaagcagag cgcgccttcg ctgtcctatt
      841 caatgtattg gggatcgaag cccccgaccg cgaacgcctt gccttctatc tgcgattgga
      901 ccctctgact tggggttgat gttcatgccg cctgtttttc ctgctcattg gcacgtttcg
      961 caacctgttc tcattgcgga caccttttcc agcctcgttt ggaaagtttc attgccagac
     1021 gggactcctg caatcgtcaa gggattgaaa cctatagaag acattgctga tgaactgcgc
     1081 ggggccgact atctggtatg gcgcaatggg aggggagcag tccggttgct cggtcgtgag
     1141 aacaatctga tgttgctcga atatgccggg gagcgaatgc tctctcacat cgttgccgag
     1201 cacggcgact accaggcgac cgaaattgca gcggaactaa tggcgaagct gtatgccgca
     1261 tctgaggaac ccctgccttc tgcccttctc ccgatccggg atcgctttgc agctttgttt
     1321 cagcgggcgc gcgatgatca aaacgcaggt tgtcaaactg actacgtcca cgcggcgatt
     1381 atagccgatc aaatgatgag caatgcctcg gaactgcgtg ggctacatgg cgatctgcat
     1441 catgaaaaca tcatgttctc cagtcgcggc tggctggtga tagatcccgt cggtctggtc
     1501 ggtgaagtgg gctttggcgc cgccaatatg ttctacgatc cggctgacag agacgacctt
     1561 tgtctcgatc ctagacgcat tgcacagatg gcggacgcat tctctcgtgc gctggacgtc
     1621 gatccgcgtc gcctgctcga ccaggcgtac gcttatgggt gcctttccgc agcttggaac
     1681 gcggatggag aagaggagca acgcgatcta gctatcgcgg ccgcgatcaa gcaggtgcga
     1741 cagacgtcat actagatatc aagcgacttc tcctatcccc tgggaacaca tcaatctcac
     1801 cggagaatat cgctggccaa agccttagcg taggattccg ccccttcccg caaacgaccc
     1861 caaacaggaa acgcagctga aacgggaagc tcaacaccca ctgacgcatg ggttgttcag
     1921 gcagtacttc atcaaccagc aaggcggcac tttcggccat ccgccgcgcc ccacagctcg
     1981 ggcagaaacc gcgacgctta cagctgaaag cgaccaggtg ctcggcgtgg caagactcgc
     2041 agcgaacccg tagaaagcca tgctccagcc gcccgcattg gagaaattct tcaaattccc
     2101 gttgcacata gcccggcaat tcctttccct gctctgccat aagcgcagcg aatgccgggt
     2161 aatactcgtc aacgatctga tagagaaggg tttgctcggg tcggtggctc tggtaacgac
     2221 cagtatcccg atcccggctg gccgtcctgg ccgccacatg aggcatgttc cgcgtccttg
     2281 caatactgtg tttacataca gtctatcgct tagcggaaag ttcttttacc ctcagccgaa
     2341 atgcctgccg ttgctagaca ttgccagcca gtgcccgtca ctcccactct tacattgttc
     2401 gcagtatcta ggtacatgtt attgcgtgca ccttcatgat gtataatgtt attaatgaca
     2461 gcagaaagat cgacagaatt aatattcttc gtacctggga cagttatcgg gtgtcggcca
     2521 gtgatgagac caaaggatgc agtataagat gaaccactac tattatcatg gccaccatta
     2581 ccaccaccgt taaaactacc tcccgaactt ccagagccgc tatgaccatc attgtagggg
     2641 tcatttggtc ccatgtgacc aaaaccaccg acagtaccgc tatttaactc tgtactcata
     2701 aaattatcct ctttgacttt taaaaacagc aaaaactgta tttataacaa tgtgtttata
     2761 tacagtattc gaaatactac gcccgtcaaa accacaccgt caacaggttt tttgtgtatt
     2821 tttcacgcct cacgggcagc ctcagacctt ttgtgttaaa acagtaatat gttctgctgt
     2881 tacaatacgg tacacacagg agacactgtc acttttatgg catggcacac agttcaagag
     2941 acactgaaat taacaggaaa gtcgcgctcg caattatatc gtgacatggc taaaggcctt
     3001 gtcagctatc gcacaggaaa aaatgatcgc cgggagttcg agaccagtga actgattcgg
     3061 gcctatggcg agctgcgcac ttctgagaca ccaaaaaggc acaaggaggg acactccaaa
     3121 tctgtcgtta gccaggcgga aaatgctcag ctagacgtca ttcagcagaa actggacgct
     3181 ctgcagcaga ctgttaccct aatgcttgaa gacaagatgg cgcgggaagc agaaaaccgc
     3241 ctgcgaaatg aggagaggga acaactacag gccgaaatta gccgtctggc tgacgcgctg
     3301 gcacaggaaa aaaagcgggg attctggtcg cggctgtttc gctccggttc agagtaacag
     3361 tgcaggtgac tctgatttac cataacatct gttatcagca ccgcctgaga ggccctcagg
     3421 gcgtttctga cgggaaaccg gtaaaacagg caacaaacca cccgaaaacc cgccagaaac
     3481 gcgcaggctc tgtttttacg gcatgcatga gtatgcgcac aagtgcatag cgcggaaatt
     3541 cacaccattc agccggggta agtcctgttt ccggcgttct tcccatccga aaacggtgaa
     3601 cggggacgca atttattgcg tggccgtgaa gcgttgccgg cgcagccgga aaacccgctg
     3661 tcagcacgtt caggaagggg gtgatgtaca aatggcacag aggatgaaag tgacgtatga
     3721 gctgaaggaa tgatgtaaaa gtgatgtata agtgatgtaa aagtgatgta
//
LOCUS       DAJTMC010000454         3770 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.guided.249, whole genome shotgun sequence.
ACCESSION   DAJTMC010000454 DAJTMC010000000
VERSION     DAJTMC010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3770
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.guided.249"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            96..569
                     /locus_tag="OO130_004437"
     CDS             96..569
                     /locus_tag="OO130_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA14"
                     /protein_id="HCR5367541.1"
                     /translation="MKVSLIAAKAKNGVIGCGPDIPWSAKGEQLLFKALTYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSGWTSNDDNVVVFQSIEEAMDRLAEFTGHVIVSGGGEI
                     YRETLPMASTLHLSTIDIEPEGDVFFPSIPNTFEVVFEQHFTSNINYCYQIWKKG"
     gene            644..919
                     /locus_tag="OO130_004438"
                     /pseudo
     CDS             644..919
                     /locus_tag="OO130_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase APH(3')"
     gene            919..1755
                     /locus_tag="OO130_004439"
     CDS             919..1755
                     /locus_tag="OO130_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APH(6)-I family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HCR5367542.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSSAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(1862..2266)
                     /locus_tag="OO130_004440"
                     /pseudo
     CDS             complement(1862..2266)
                     /locus_tag="OO130_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(2310..2699)
                     /locus_tag="OO130_004441"
     CDS             complement(2310..2699)
                     /locus_tag="OO130_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5367543.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            2917..3357
                     /locus_tag="OO130_004442"
     CDS             2917..3357
                     /locus_tag="OO130_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HCR5367544.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
BASE COUNT          955 a          980 c          964 g          871 t
ORIGIN      
        1 aagagtacgc cgcagctcga tcttttggct cgtgtcgaac tagagctacc ggtgcggctc
       61 gaccaagagc gcaccgttaa cccaggatga gaaccttgaa agtatcattg atagctgcga
      121 aagcgaaaaa cggcgtgatt ggttgcggtc cagacatacc ctggtccgcg aaaggggagc
      181 agctactttt taaagcattg acctacaatc agtggcttct ggtgggtcgc aagacgtttg
      241 aatctatggg cgcactcccc aataggaaat acgcggtcgt tacccgctca ggttggacat
      301 caaatgatga caatgtagtt gtatttcagt caatcgaaga ggccatggac aggctagctg
      361 aattcaccgg tcacgttata gtgtctggtg gcggagaaat ttaccgagaa acattaccca
      421 tggcctctac gctccactta tcgacgatcg acatcgagcc agagggggat gttttcttcc
      481 cgagtattcc aaataccttc gaagttgttt ttgagcaaca ctttacttca aacattaact
      541 attgctatca aatttggaaa aagggttaac aaagctatgc aatcgacggc aaaaagcttc
      601 gttcgcttcg cgcactacgc ctttttccgc gattgatagc gacgatatgg ttgtttgcca
      661 tggtgatccc tgcatgccga acttcatggt ggaccctaaa actcttcaat gcacgggtct
      721 gatcgacctt gggcggctcg gaacagcaga tcgctatgcc gatttggcac tcatgattgc
      781 taacgccgaa gagaactggg cagcgccaga tgaagcagag cgcgccttcg ctgtcctatt
      841 caatgtattg gggatcgaag cccccgaccg cgaacgcctt gccttctatc tgcgattgga
      901 ccctctgact tggggttgat gttcatgccg cctgtttttc ctgctcattg gcacgtttcg
      961 caacctgttc tcattgcgga caccttttcc agcctcgttt ggaaagtttc attgccagac
     1021 gggactcctg caatcgtcaa gggattgaaa cctatagaag acattgctga tgaactgcgc
     1081 ggggccgact atctggtatg gcgcaatggg aggggagcag tccggttgct cggtcgtgag
     1141 aacaatctga tgttgctcga atatgccggg gagcgaatgc tctctcacat cgttgccgag
     1201 cacggcgact accaggcgac cgaaattgca gcggaactaa tggcgaagct gtatgccgca
     1261 tctgaggaac ccctgccttc tgcccttctc ccgatccggg atcgctttgc agctttgttt
     1321 cagcgggcgc gcgatgatca aaacgcaggt tgtcaaactg actacgtcca cgcggcgatt
     1381 atagccgatc aaatgatgag caatgcctcg gaactgcgtg ggctacatgg cgatctgcat
     1441 catgaaaaca tcatgttctc cagtcgcggc tggctggtga tagatcccgt cggtctggtc
     1501 ggtgaagtgg gctttggcgc cgccaatatg ttctacgatc cggctgacag agacgacctt
     1561 tgtctcgatc ctagacgcat tgcacagatg gcggacgcat tctctcgtgc gctggacgtc
     1621 gatccgcgtc gcctgctcga ccaggcgtac gcttatgggt gcctttcctc agcttggaac
     1681 gcggatggag aagaggagca acgcgatcta gctatcgcgg ccgcgatcaa gcaggtgcga
     1741 cagacgtcat actagatatc aagcgacttc tcctatcccc tgggaacaca tcaatctcac
     1801 cggagaatat cgctggccaa agccttagcg taggattccg ccccttcccg caaacgaccc
     1861 caaacaggaa acgcagctga aacgggaagc tcaacaccca ctgacgcatg ggttgttcag
     1921 gcagtacttc atcaaccagc aaggcggcac tttcggccat ccgccgcgcc ccacagctcg
     1981 ggcagaaacc gcgacgctta cagctgaaag cgaccaggtg ctcggcgtgg caagactcgc
     2041 agcgaacccg tagaaagcca tgctccagcc gcccgcattg gagaaattct tcaaattccc
     2101 gttgcacata gcccggcaat tcctttccct gctctgccat aagcgcagcg aatgccgggt
     2161 aatactcgtc aacgatctga tagagaaggg tttgctcggg tcggtggctc tggtaacgac
     2221 cagtatcccg atcccggctg gccgtcctgg ccgccacatg aggcatgttc cgcgtccttg
     2281 caatactgtg tttacataca gtctatcgct tagcggaaag ttcttttacc ctcagccgaa
     2341 atgcctgccg ttgctagaca ttgccagcca gtgcccgtca ctcccactct tacattgttc
     2401 gcagtatcta ggtacatgtt attgcgtgca ccttcatgat gtataatgtt attaatgaca
     2461 gcagaaagat cgacagaatt aatattcttc gtacctggga cagttatcgg gtgtcggcca
     2521 gtgatgagac caaaggatgc agtataagat gaaccactac tattatcatg gccaccatta
     2581 ccaccaccgt taaaactacc tcccgaactt ccagagccgc tatgaccatc attgtagggg
     2641 tcatttggtc ccatgtgacc aaaaccaccg acagtaccgc tatttaactc tgtactcata
     2701 aaattatcct ctttgacttt taaaaacagc aaaaactgta tttataacaa tgtgtttata
     2761 tacagtattc gaaatactac gcccgtcaaa accacaccgt caacaggttt tttgtgtatt
     2821 tttcacgcct cacgggcagc ctcagacctt ttgtgttaaa acagtaatat gttctgctgt
     2881 tacaatacgg tacacacagg agacactgtc acttttatgg catggcacac agttcaagag
     2941 acactgaaat taacaggaaa gtcgcgctcg caattatatc gtgacatggc taaaggcctt
     3001 gtcagctatc gcacaggaaa aaatgatcgc cgggagttcg agaccagtga actgattcgg
     3061 gcctatggcg agctgcgcac ttctgagaca ccaaaaaggc acaaggaggg acactccaaa
     3121 tctgtcgtta gccaggcgga aaatgctcag ctagacgtca ttcagcagaa actggacgct
     3181 ctgcagcaga ctgttaccct aatgcttgaa gacaagatgg cgcgggaagc agaaaaccgc
     3241 ctgcgaaatg aggagaggga acaactacag gccgaaatta gccgtctggc tgacgcgctg
     3301 gcacaggaaa aaaagcgggg attctggtcg cggctgtttc gctccggttc agagtaacag
     3361 tgcaggtgac tctgatttac cataacatct gttatcagca ccgcctgaga ggccctcagg
     3421 gcgtttctga cgggaaaccg gtaaaacagg caacaaacca cccgaaaacc cgccagaaac
     3481 gcgcaggctc tgtttttacg gcatgcatga gtatgcgcac aagtgcatag cgcggaaatt
     3541 cacaccattc agccggggta agtcctgttt ccggcgttct tcccatccga aaacggtgaa
     3601 cggggacgca atttattgcg tggccgtgaa gcgttgccgg cgcagccgga aaacccgctg
     3661 tcagcacgtt caggaagggg gtgatgtaca aatggcacag aggatgaaag tgacgtatga
     3721 gctgaaggaa tgatgtaaaa gtgatgtata agtgatgtaa aagtgatgta
//
LOCUS       DAJTMC010000455         3423 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.guided.260, whole genome shotgun sequence.
ACCESSION   DAJTMC010000455 DAJTMC010000000
VERSION     DAJTMC010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3423
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.guided.260"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            144..548
                     /locus_tag="OO130_004443"
     CDS             144..548
                     /locus_tag="OO130_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000512977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HCR5367545.1"
                     /translation="MGISLNHGVHAKVSTPVHLRKARTCYDHLAGEVAVKIYDSLCQQ
                     QWITENGSMITLSGIQYFHEMGIDVPSKHSRKICCACLDWSERRFHLGGYVGAALFSL
                     YESKGWLTRHLGYREVTITEKGYAAFKTHFHI"
     gene            complement(526..1149)
                     /gene="tetR(B)"
                     /locus_tag="OO130_004444"
     CDS             complement(526..1149)
                     /gene="tetR(B)"
                     /locus_tag="OO130_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HCR5367546.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            1231..2436
                     /gene="tet(B)"
                     /locus_tag="OO130_004445"
     CDS             1231..2436
                     /gene="tet(B)"
                     /locus_tag="OO130_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HCR5367547.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(2549..3142)
                     /gene="tetC"
                     /locus_tag="OO130_004446"
     CDS             complement(2549..3142)
                     /gene="tetC"
                     /locus_tag="OO130_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HCR5367548.1"
                     /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL
                     VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIID
                     NNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLN
                     GWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
BASE COUNT          925 a          630 c          705 g         1163 t
ORIGIN      
        1 ctgtggcgaa tatatcagct tcaacggcga gcagtcattt atctaaatta ttagattgcc
       61 agctaatcac agtagtagct caaggcaagc atcgttattt tcggctagca ggaaaagata
      121 ttgctgaatt gatggaaagt atgatgggga tctccttaaa ccatggcgta catgccaaag
      181 tttccacgcc agtgcattta cgaaaagcac gtacttgcta tgatcattta gctggcgaag
      241 ttgccgttaa gatctatgat tccctttgtc aacagcaatg gatcactgaa aatggttcaa
      301 tgatcacatt aagtggtatt caatattttc atgaaatggg aattgacgtt ccttccaaac
      361 attcacgtaa aatctgttgt gcgtgtttag attggagtga acgccgtttc catttaggtg
      421 ggtacgttgg agccgcatta ttttcgcttt atgaatctaa agggtggtta actcgacatc
      481 ttggttaccg tgaagttacc atcacggaaa aaggttatgc tgcttttaag acccactttc
      541 acatttaagt tgtttttcta atccgcatat gatcaattca aggccgaata agaaggctgg
      601 ctctgcacct tggtgatcaa ataattcgat agcttgtcgt aataatggcg gcatactatc
      661 agtagtaggt gtttcccttt cttctttagc gacttgatgc tcttgatctt ccaatacgca
      721 acctaaagta aaatgcccca cagcgctgag tgcatataat gcattctcta gtgaaaaacc
      781 ttgttggcat aaaaaggcta attgattttc gagagtttca tactgttttt ctgtaggccg
      841 tgtacctaaa tgtacttttg ctccatcgcg atgacttagt aaagcacatc taaaactttt
      901 agcgttatta cgtaaaaaat cttgccagct ttccccttct aaagggcaaa agtgagtatg
      961 gtgcctatct aacatctcaa tggctaaggc gtcgagcaaa gcccgcttat tttttacatg
     1021 ccaatacaat gtaggctgct ctacacctag cttctgggcg agtttacggg ttgttaaacc
     1081 ttcgattccg acctcattaa gcagctctaa tgcgctgtta atcactttac ttttatctaa
     1141 tctagacatc attaattcct aatttttgtt gacactctat cattgataga gttattttac
     1201 cactccctat cagtgataga gaaaagtgaa atgaatagtt cgacaaagat cgcattggta
     1261 attacgttac tcgatgccat ggggattggc cttatcatgc cagtcttgcc aacgttatta
     1321 cgtgaattta ttgcttcgga agatatcgct aaccactttg gcgtattgct tgcactttat
     1381 gcgttaatgc aggttatctt tgctccttgg cttggaaaaa tgtctgaccg atttggtcgg
     1441 cgcccagtgc tgttgttgtc attaataggc gcatcgctgg attacttatt gctggctttt
     1501 tcaagtgcgc tttggatgct gtatttaggc cgtttgcttt cagggatcac aggagctact
     1561 ggggctgtcg cggcatcggt cattgccgat accacctcag cttctcaacg cgtgaagtgg
     1621 ttcggttggt taggggcaag ttttgggctt ggtttaatag cggggcctat tattggtggt
     1681 tttgcaggag agatttcacc gcatagtccc ttttttatcg ctgcgttgct aaatattgtc
     1741 gctttccttg tggttatgtt ttggttccgt gaaaccaaaa atacacgtga taatacagat
     1801 accgaagtag gggttgagac gcaatcgaat tcggtataca tcactttatt taaaacgatg
     1861 cccattttgt tgattattta tttttcagcg caattgatag gccaaattcc cgcaacggtg
     1921 tgggtgctat ttaccgaaaa tcgttttgga tggaatagca tgatggttgg cttttcatta
     1981 gcgggtcttg gtcttttaca ctcagtattc caagcctttg tggcaggaag aatagccact
     2041 aaatggggcg aaaaaacggc agtactgctc ggatttattg cagatagtag tgcatttgcc
     2101 tttttagcgt ttatatctga aggttggtta gttttccctg ttttaatttt attggctggt
     2161 ggtgggatcg ctttacctgc attacaggga gtgatgtcta tccaaacaaa gagtcatcag
     2221 caaggtgctt tacagggatt attggtgagc cttaccaatg caaccggtgt tattggccca
     2281 ttactgtttg ctgttattta taatcattca ctaccaattt gggatggctg gatttggatt
     2341 attggtttag cgttttactg tattattatc ctgctatcga tgaccttcat gttaacccct
     2401 caagctcagg ggagtaaaca ggagacaagt gcttagttat ttcgtcacca aatgatgtta
     2461 ttccgcgaaa tataatgacc ctcttgataa cccaagaggg cattttttac gataaagaag
     2521 atttagcttc aaataaaacc tatctatttt atttatcttt caagctcaat aaaaagccgc
     2581 ggtaaatagc aataaattgg ccttttttat cggcaagctc ttttaggttt ttcgcatgta
     2641 ttgcgatatg cataaaccag ccattgagta agtttttaag cacatcatca tcataagctt
     2701 taagttggtt ctcttggatc aatttgctga caatggcgtt taccttacca gtaatgtatt
     2761 caaggctaat tttttcaagt tcattccaac caatgatagg catcacttct tggataggga
     2821 taaggttttt attattatca ataatataat caagataatg ttcaaatata ctttctaagg
     2881 cagaccaacc atttgttaaa tcagtttttg ttgtgatgta ggcatcaatc ataattaatt
     2941 gctgcttata acaggcactg agtaattgtt ttttattttt aaagtgatga taaaaggcac
     3001 ctttggtcac caacgctttt cccgagatct catctattga aacagcttga tagccttttt
     3061 caacaaacaa tattcgtgct gagttaacca gtgattgata ggtactctta aaattttctt
     3121 gttgatgatt tttattttcc atgatagatt taaaataaca taccgtcagt atgtttatgg
     3181 tatcatgatg atgtggtcgt gacaatctta agaacattta ggttatttta tgtatattga
     3241 acagcattct cgctatcaaa ataaagctaa taacatccaa ttaagatatg atgataagca
     3301 gtttcataca acggttatca aagatgttct attatggatt gaacataatt tagatcagtc
     3361 tttactgctt gatgatgtgg cgaataaagc gggttatacc aagtggtatt ttcagcggct
     3421 gtt
//
LOCUS       DAJTMC010000456         2422 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.guided.294, whole genome shotgun sequence.
ACCESSION   DAJTMC010000456 DAJTMC010000000
VERSION     DAJTMC010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2422
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.guided.294"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            980..1795
                     /gene="sul2"
                     /locus_tag="OO130_004447"
     CDS             980..1795
                     /gene="sul2"
                     /locus_tag="OO130_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HCR5367549.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            1856..>2422
                     /locus_tag="OO130_004448"
     CDS             1856..>2422
                     /locus_tag="OO130_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APH(3'') family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HCR5367550.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPN"
BASE COUNT          525 a          669 c          658 g          570 t
ORIGIN      
        1 tgactcgcta cgctcggtcg ttcggctgcg gcgagcggta ccggcttacg gacggggacg
       61 gaaatttcct ggaagatacc aggaagacac ttagcaaaga agagataagg ccacggcgaa
      121 gccgtttttc cataggctcc gcccccctga caagcatcgc aaaatctgac gctcaaatca
      181 gtggtggcga aacccgacag gactataaag atcccaggcg tttccccctg gtagctccct
      241 cgtgcgctct cctgttcctg cctttcggtt taccggtgtc attccgctgt tatggccgcg
      301 gtttctcatt ccacgcctga cactcagttc cgtgaaggca gttcgctcca agctggactg
      361 tatgcacgaa ccccccgttc agtccgaccg ctgcgccttg tccggtaact atcaacttga
      421 gtccaacccg gaaagacacg acaaaacgcc actggcagca gccattagta acaggagtgc
      481 acgagaagat agattaagag gtcttgaagt agaggcctga ctgcggctac actgaaagaa
      541 cagttttggt aatggcggtc tcgtgaaccc tgttaccggg ttaagcagtt ccccaactga
      601 cttaaccttc gaacaaaccg cctccccagg cggttttttc gtttacagag caagagatta
      661 cgattcattg aaaaggatct caagaagatc ctttgtagat tactatcaat taatcttccg
      721 aacatgacac cagtcactgt tgattacggt taaaccgctc cgtaacaggt ttccctcgta
      781 ttggtgactt gcagcaccaa agaagcaaaa aagataaata tatctactta ttgagagatg
      841 atatatttat ttccaaaatg ctacagatac atcatcatga aacggatcac cgcaaacagg
      901 ttactcgcct ggggattccc tttcgacccg agcatccgta tgagactcat gctcgattat
      961 tattattata gaagccccca tgaataaatc gctcatcatt ttcggcatcg tcaacataac
     1021 ctcggacagt ttctccgatg gaggccggta tctggcgcca gacgcagcca ttgcgcaggc
     1081 gcgtaagctg atggccgagg gggcagatgt gatcgacctc ggtccggcat ccagcaatcc
     1141 cgacgccgcg cctgtttcgt ccgacacaga aatcgcgcgt atcgcgccgg tgctggacgc
     1201 gctcaaggca gatggcattc ccgtctcgct cgacagttat caacccgcga cgcaagccta
     1261 tgccttgtcg cgtggtgtgg cctatctcaa tgatattcgc ggttttccag acgctgcgtt
     1321 ctatccgcaa ttggcgaaat catctgccaa actcgtcgtt atgcattcgg tgcaagacgg
     1381 gcaggcagat cggcgcgagg cacccgctgg cgacatcatg gatcacattg cggcgttctt
     1441 tgacgcgcgc atcgcggcgc tgacgggtgc cggtatcaaa cgcaaccgcc ttgtccttga
     1501 tcccggcatg gggttttttc tgggggctgc tcccgaaacc tcgctctcgg tgctggcgcg
     1561 gttcgatgaa ttgcggctgc gcttcgattt gccggtgctt ctgtctgttt cgcgcaaatc
     1621 ctttctgcgc gcgctcacag gccgtggtcc gggggatgtc ggggccgcga cactcgctgc
     1681 agagcttgcc gccgccgcag gtggagctga cttcatccgc acacacgagc cgcgcccctt
     1741 gcgcgacggg ctggcggtat tggcggcgct gaaagaaacc gcaagaattc gttaactgca
     1801 cattcgggat atttctctat attcgcgctt catcagaaaa ctgaaggaac ctccattgaa
     1861 tcgaactaat attttttttg gtgaatcgca ttctgactgg ttgcctgtca gaggcggaga
     1921 atctggtgat tttgtttttc gacgtggtga cgggcatgcc ttcgcgaaaa tcgcacctgc
     1981 ttcccgccgc ggtgagctcg ctggagagcg tgaccgcctc atttggctca aaggtcgagg
     2041 tgtggcttgc cccgaggtca tcaactggca ggaggaacag gagggtgcat gcttggtgat
     2101 aacggcaatt ccgggagtac cggcggctga tctgtctgga gcggatttgc tcaaagcgtg
     2161 gccgtcaatg gggcagcaac ttggcgctgt tcacagccta tcggttgatc aatgtccgtt
     2221 tgagcgcagg ctgtcgcgaa tgttcggacg cgccgttgat gtggtgtccc gcaatgccgt
     2281 caatcccgac ttcttaccgg acgaggacaa gagtacgccg cagctcgatc ttttggctcg
     2341 tgtcgaacga gagctaccgg tgcggctcga ccaagagcgc accgatatgg ttgtttgcca
     2401 tggtgatccc tgcatgccga ac
//
LOCUS       DAJTMC010000457         1681 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.guided.317, whole genome shotgun sequence.
ACCESSION   DAJTMC010000457 DAJTMC010000000
VERSION     DAJTMC010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1681
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.guided.317"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            329..787
                     /gene="gpt"
                     /locus_tag="OO130_004449"
     CDS             329..787
                     /gene="gpt"
                     /locus_tag="OO130_004449"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCR5367551.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            879..>1681
                     /gene="frsA"
                     /locus_tag="OO130_004450"
     CDS             879..>1681
                     /gene="frsA"
                     /locus_tag="OO130_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HCR5367552.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIVMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVA"
BASE COUNT          406 a          423 c          443 g          409 t
ORIGIN      
        1 ggaatagaac agattttggc aaaaatatcc cacagcggct gtggagataa ttgagacagt
       61 tcagacacgt taagtctcct tgtcgatcac ccgcaaaaca gtattgcagg tcacagggtt
      121 agcagaaaat gttgtcaaca caagacaggc ttgcgagata tgtttgagaa taccacttta
      181 tcccgcgtca gggagaggca gtgcgtaaaa agacgcggac tcatgtgaaa tactggtttt
      241 tagtgcgcca gatctctata atctcgcgca acctattttc ccctcgaaca ctttttaagc
      301 cgtagataaa caggctggga cacttcacat gagcgaaaaa tacatcgtca cctgggacat
      361 gttgcagatc catgcacgta aactcgcaag ccgactgatg ccttctgaac aatggaaagg
      421 cattattgcc gtaagccgtg gcggtctggt accgggtgcg ttactggcgc gtgaactggg
      481 tattcgtcat gtcgataccg tttgtatttc cagctacgat cacgacaacc agcgagagct
      541 taaagtgctg aaacgcgcag aaggcgatgg cgaaggcttc atagttattg atgacctggt
      601 ggataccggt ggtactgctg ttgcgattcg tgaaatgtat ccaaaagcgc actttgtcac
      661 catcttcgca aaaccggctg gtcgtccgct ggttgatgac tatgttgttg atattccgca
      721 agatacctgg atcgaacagc cgtgggatat gggcgtcgta ttcgtcccgc caatctccgg
      781 tcgctaatct tttcaacgcc tggcactgcc gggcgttgtt ctttttaact tcaggcaggt
      841 tacaatagtt tccagtaagt attctggagg ctgcatccat gacacaggca aacctgagcg
      901 aaaccctgtt caaaccccgc tttaaacatc ctgaaacctc gacgctagtc cgccgcttta
      961 atcacggcgc acaaccgcct gtgcagtcgg ctcttgatgg taaaaccatc cctcactggt
     1021 atcgcatgat taaccgtctg atgtggatct ggcgcggtat tgacccacgt gaaatcctcg
     1081 acgtccaggc acgtattgtg atgagcgatg ctgaacgtac cgacgatgat ttatacgata
     1141 ctgtgattgg ctaccgtggt ggcaactgga tttatgagtg ggccacccag gcgatggtgt
     1201 ggcaacaaaa agcctgtgcg gaagaagatc cgcaactcag tggtcgacac tggctgcatg
     1261 cggctacgtt gtacaacatt gccgcctatc ctcatctgaa aggagatgac ctggccgagc
     1321 aagcgcaggc tttatcaaac cgcgcctatg aagaggccgc ccagcgtctg ccgggcacga
     1381 tgcggcagat ggagtttacc gtacccggcg gtgcgcccat caccggcttt ttgcatatgc
     1441 cgaaaggcga tggcccgttc ccgacagtat taatgtgtgg tggtctggat gcgatgcaga
     1501 cggactatta cagcctgtat gaacgttatt ttgcgccgcg cggcattgtg atgctgacta
     1561 ttgatatgcc gtcggtgggc ttttcttcaa aatggaagct cacccaggac tccagcctgt
     1621 tgcatcagca cgtcttaaag gcgctgccta acgtaccgtg ggtggatcac actcgcgtcg
     1681 c
//
LOCUS       DAJTMC010000458         1574 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.guided.325, whole genome shotgun sequence.
ACCESSION   DAJTMC010000458 DAJTMC010000000
VERSION     DAJTMC010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.guided.325"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            13..372
                     /gene="frdD"
                     /locus_tag="OO130_004451"
     CDS             13..372
                     /gene="frdD"
                     /locus_tag="OO130_004451"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HCR5367553.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            435..1568
                     /locus_tag="OO130_004452"
     CDS             435..1568
                     /locus_tag="OO130_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HCR5367554.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQMPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLTPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
BASE COUNT          384 a          399 c          410 g          381 t
ORIGIN      
        1 aaggagcctg agatgattaa tccaaatcca aagcgttctg acgaaccggt attctggggc
       61 ctcttcgggg ccggtggtat gtggagcgca attattgcgc cggtgatgat cctgctggtg
      121 ggtattctgc tgccactggg gctgtttccg ggtgatgcgc tgagctacga gcgcgttctg
      181 gcgttcgcgc agagcttcat tggtcgcgta ttcctgttcc tgatgatcgt tctgccgctg
      241 tggtgtggtt tacaccgtat gcaccacgcg atgcacgatc tgaaaattca cgtgcctgcg
      301 ggcaaatggg ttttctacgg tctggctgct atcctgacag ttgtcacgct gattggtatc
      361 gttacaatct aacgtatcgc caatgtaaat ccggcccgcc tatggcgggc cgttttgtat
      421 ggaaaccaga ccttatgttc aaaacgacgc tctgcgcctt attaattacc gcctcttgct
      481 ccacatttgc tgcccctcaa caaatcaacg atattgtgca tcgcacaatt accccgctta
      541 tagagcaaca aaagatcccc ggtatggcgg tggcggtaat ttatcagggt aaaccttatt
      601 actttacctg gggctatgcg gacatcgcca aaaagcagcc cgtcacacag caaacgttgt
      661 ttgagttagg ttcggtcagc aaaacattta cgggcgtgct tggtggcgat gctattgctc
      721 gaggggaaat caagttaagc gatcccgcaa caaaatactg gcctgaactt accgctaaac
      781 agtggaatgg gatcacacta ttacatcttg cgacctacac cgctggcggc ctgccattgc
      841 agatgccgga tgaagtgaaa tcctcaagcg acttgctgcg cttctatcaa aactggcagc
      901 ctgcatgggc tccaggaaca caacgtctgt atgccaactc cagtatcggt ttgttcggcg
      961 cactggctgt gaagccgtct ggtttgagtt ttgagcaggc gatgcaaact cgtgtcttcc
     1021 agccactcaa actcaaccat acgtggatta atgtaccgcc cgcagaagaa aagaattacg
     1081 cctggggata tcgcgaaggc aaggcagttc atgtttcgcc aggggcgtta gatgctgaag
     1141 cttatggtgt gaagtcgacc attgaagata tggcccgctg ggtgcaaagc aatttaaccc
     1201 cccttgatat caatgagaaa acgcttcaac aagggataca actggcacaa tctcgctact
     1261 ggcaaaccgg cgatatgtat cagggcctgg gctgggaaat gctggactgg ccggtcaatc
     1321 ctgacagcat cattaacggc agtgacaata aaattgcact ggcagcacgc ccggtaaaag
     1381 cgattaagcc cccaactcct gcagtacgcg catcatgggt acataaaacg ggggcgaccg
     1441 gcggatttgg tagctatgtc gcgtttattc cagaaaaaga gctgggtatc gtgatgctgg
     1501 ctaacaaaaa ctatcccaat ccagcgagag tcgccgccgc ctgtcagatt ctcaacgctc
     1561 tacagtaaaa tttc
//
LOCUS       DAJTMC010000459         1571 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 600832
            SAMEA8903754-rid15821373.guided.327, whole genome shotgun sequence.
ACCESSION   DAJTMC010000459 DAJTMC010000000
VERSION     DAJTMC010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8903754
            Sequence Read Archive: ERR6006283
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001478913.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001478913.1
            Genome Coverage       :: 373x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:56:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,454
            CDSs (total)                      :: 4,361
            Genes (coding)                    :: 3,994
            CDSs (with protein)               :: 3,994
            Genes (RNA)                       :: 93
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 2, 1 (16S, 23S)
            tRNAs                             :: 83
            ncRNAs                            :: 6
            Pseudo Genes (total)              :: 367
            CDSs (without protein)            :: 367
            Pseudo Genes (ambiguous residues) :: 0 of 367
            Pseudo Genes (frameshifted)       :: 195 of 367
            Pseudo Genes (incomplete)         :: 105 of 367
            Pseudo Genes (internal stop)      :: 106 of 367
            Pseudo Genes (multiple problems)  :: 37 of 367
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1571
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8903754-rid15821373.guided.327"
                     /serovar="S. flexneri 6"
                     /isolate="600832"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="Bangladesh"
                     /collection_date="2008-08-02"
     gene            13..372
                     /gene="frdD"
                     /locus_tag="OO130_004453"
     CDS             13..372
                     /gene="frdD"
                     /locus_tag="OO130_004453"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HCR5367555.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            435..1565
                     /locus_tag="OO130_004454"
     CDS             435..1565
                     /locus_tag="OO130_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HCR5367556.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQMPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLTPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAACQILNALQ"
BASE COUNT          384 a          397 c          409 g          381 t
ORIGIN      
        1 aaggagcctg agatgattaa tccaaatcca aagcgttctg acgaaccggt attctggggc
       61 ctcttcgggg ccggtggtat gtggagcgca attattgcgc cggtgatgat cctgctggtg
      121 ggtattctgc tgccactggg gctgtttccg ggtgatgcgc tgagctacga gcgcgttctg
      181 gcgttcgcgc agagcttcat tggtcgcgta ttcctgttcc tgatgatcgt tctgccgctg
      241 tggtgtggtt tacaccgtat gcaccacgcg atgcacgatc tgaaaattca cgtgcctgcg
      301 ggcaaatggg ttttctacgg tctggctgct atcctgacag ttgtcacgct gattggtatc
      361 gttacaatct aacgtatcgc caatgtaaat ccggcccgcc tatggcgggc cgttttgtat
      421 ggaaaccaga ccttatgttc aaaacgacgc tctgcgcctt attaattacc gcctcttgct
      481 ccacatttgc tgcccctcaa caaatcaacg atattgtgca tcgcacaatt accccgctta
      541 tagagcaaca aaagatcccc ggtatggcgg tggcggtaat ttatcagggt aaaccttatt
      601 actttacctg gggctatgcg gacatcgcca aaaagcagcc cgtcacacag caaacgttgt
      661 ttgagttagg ttcggtcagc aaaacattta cgggcgtgct tggtggcgat gctattgctc
      721 gaggggaaat caagttaagc gatcccgcaa caaaatactg gcctgaactt accgctaaac
      781 agtggaatgg gatcacacta ttacatcttg cgacctacac cgctggcggc ctgccattgc
      841 agatgccgga tgaagtgaaa tcctcaagcg acttgctgcg cttctatcaa aactggcagc
      901 ctgcatgggc tccaggaaca caacgtctgt atgccaactc cagtatcggt ttgttcggcg
      961 cactggctgt gaagccgtct ggtttgagtt ttgagcaggc gatgcaaact cgtgtcttcc
     1021 agccactcaa actcaaccat acgtggatta atgtaccgcc cgcagaagaa aagaattacg
     1081 cctggggata tcgcgaaggc aaggcagttc atgtttcgcc aggggcgtta gatgctgaag
     1141 cttatggtgt gaagtcgacc attgaagata tggcccgctg ggtgcaaagc aatttaaccc
     1201 cccttgatat caatgagaaa acgcttcaac aagggataca actggcacaa tctcgctact
     1261 ggcaaaccgg cgatatgtat cagggcctgg gctgggaaat gctggactgg ccggtcaatc
     1321 ctgacagcat cattaacggc agtgacaata aaattgcact ggcagcacgc ccggtaaaag
     1381 cgattaagcc cccaactcct gcagtacgcg catcatgggt acataaaacg ggggcgaccg
     1441 gcggatttgg tagctatgtc gcgtttattc cagaaaaaga gctgggtatc gtgatgctgg
     1501 ctaacaaaaa ctatcccaat ccagcgagag tcgccgcctg tcagattctc aacgctctac
     1561 agtaaaattt c
//
